LOCUS       DAMJVM010000001       125351 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.001, whole genome shotgun sequence.
ACCESSION   DAMJVM010000001 DAMJVM010000000
VERSION     DAMJVM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 125351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 125351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..125351
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.001"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            118..309
                     /locus_tag="PC523_000001"
     CDS             118..309
                     /locus_tag="PC523_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDF2473577.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMISRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            464..1873
                     /locus_tag="PC523_000002"
     CDS             464..1873
                     /locus_tag="PC523_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2473578.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKRGEVYKKLGYKDILENLYKM
                     DTTEILGTDNLHFPEGIIKNSQEKASKVFKSDYTYYLVNGSSCGIQSAIMSVCSPKSK
                     IIANRDCHQSVINGCILGDIDIEYVPCEISEDTNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKNMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPSFTQSSMIHIQGNRVNIDKILNMLRMTESSSPSYLLLSSLELAVDIYENKGK
                     GLMEELLNNIRTFKNNIDSNIKMYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTTKDFSSLETALNNVVKEEFKFTKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKICGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVYLNFIQVIDLEYI"
     gene            1994..2683
                     /locus_tag="PC523_000003"
     CDS             1994..2683
                     /locus_tag="PC523_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDF2473579.1"
                     /translation="MKGKLIIIESGSDASGKATQTKILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIVIADRY
                     TTSNMVHQASKMDVCDREKYLNWLADYEFNLFKIPQPDCVVFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLQKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKSMER"
     gene            2686..3621
                     /locus_tag="PC523_000004"
     CDS             2686..3621
                     /locus_tag="PC523_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDF2473580.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHRDYKIYIINNAEK
                     MTLEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKSYLI
                     DTGIEEERAQLLATFSRGSIENALNLSQSSEFSVMREDVQQYIQIMLDKNIIEILNIP
                     NNMEKYRGKIITLLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDVKNKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3618..4511
                     /locus_tag="PC523_000005"
     CDS             3618..4511
                     /locus_tag="PC523_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDF2473581.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVFLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4589..5335
                     /locus_tag="PC523_000006"
     CDS             4589..5335
                     /locus_tag="PC523_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDF2473582.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSVKLNGLEERVEIINED
                     IKYIDKVLDVNGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     SRGKFLMIHRPTRLVDIITLGRKYRLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNEDIGEL"
     gene            5336..6169
                     /gene="rsmI"
                     /locus_tag="PC523_000007"
     CDS             5336..6169
                     /gene="rsmI"
                     /locus_tag="PC523_000007"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDF2473583.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRILKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGEYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHKFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMFKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDELTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6192..6896
                     /locus_tag="PC523_000008"
     CDS             6192..6896
                     /locus_tag="PC523_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDF2473584.1"
                     /translation="MEIGKIIIESLKREDTHKTFIELDDKHELEKIIPKVSCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNNKDFFPIHLRKHVSNYLNTYIEEGINNLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGINLKLSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSEEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7084..7512)
                     /locus_tag="PC523_000009"
     CDS             complement(7084..7512)
                     /locus_tag="PC523_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDF2473585.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7549..8979)
                     /locus_tag="PC523_000010"
     CDS             complement(7549..8979)
                     /locus_tag="PC523_000010"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDF2473586.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLKYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGICKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9111..9695
                     /locus_tag="PC523_000011"
     CDS             9111..9695
                     /locus_tag="PC523_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HDF2473587.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTVIAIITCKFFQRRYR"
     gene            9711..10232
                     /locus_tag="PC523_000012"
     CDS             9711..10232
                     /locus_tag="PC523_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HDF2473588.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10298..10545
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10629..12566
                     /gene="metG"
                     /locus_tag="PC523_000013"
     CDS             10629..12566
                     /gene="metG"
                     /locus_tag="PC523_000013"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDF2473589.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDEAKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKILSCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12596..13366
                     /locus_tag="PC523_000014"
     CDS             12596..13366
                     /locus_tag="PC523_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDF2473590.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIDTSI
                     TAIELAKKYDFIYSAVGVHPHEVSKLDDTAIEILRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKSPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13704..13975
                     /locus_tag="PC523_000015"
                     /pseudo
     CDS             13704..13975
                     /locus_tag="PC523_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
     gene            14137..14973
                     /locus_tag="PC523_000016"
     CDS             14137..14973
                     /locus_tag="PC523_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF2473591.1"
                     /translation="MLTELHCHTNISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEAIVLGEHYGITIIPGIEISAYDYINRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLRYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            14974..15969
                     /locus_tag="PC523_000017"
     CDS             14974..15969
                     /locus_tag="PC523_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2473592.1"
                     /translation="MSFLQVKNVCKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDRDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKKVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDNGINKIAIRPEDIKVEFNRDSKDKDIKEGEILDIE
                     FRGFNYRITVEYCSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            15987..17345
                     /locus_tag="PC523_000018"
     CDS             15987..17345
                     /locus_tag="PC523_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2473593.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLASLLKNAFM
                     DTSGNFIGILNFSKYIENPSLIASFKNSIFVSIVSSLISLCLAFIYSYAITRTNIKFK
                     NFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKSRDRAFLIVCSLITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAVFGTIFVFFSAYMIEKVDKFPKLRQIAYFLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNIVHFYSVCFITSNSSLKMLDI
                     EV"
     gene            17336..17680
                     /locus_tag="PC523_000019"
                     /pseudo
     CDS             17336..17680
                     /locus_tag="PC523_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
     gene            17872..18402
                     /gene="rnmV"
                     /locus_tag="PC523_000020"
     CDS             17872..18402
                     /gene="rnmV"
                     /locus_tag="PC523_000020"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDF2473594.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQIDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREAFIDALKQI"
     gene            18573..19442
                     /gene="rsmA"
                     /locus_tag="PC523_000021"
     CDS             18573..19442
                     /gene="rsmA"
                     /locus_tag="PC523_000021"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDF2473595.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITGGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLENTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EEIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREILKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            19508..20770
                     /locus_tag="PC523_000022"
     CDS             19508..20770
                     /locus_tag="PC523_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473596.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEELEEEDEEVEDNEESNRDEDIEEEEY
                     IIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSSNEEIEEFNNYKEIENYNRVKLQE
                     EYIDIECEEDEEEDEEEVFEVNREDDRFVSSKPTKSKKVQKEQNNIEKIKRVNGVSQG
                     ESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYLG
                     YLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGFL
                     RYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            20921..22036
                     /locus_tag="PC523_000023"
     CDS             20921..22036
                     /locus_tag="PC523_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2473597.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVV
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEVNGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            22222..23118
                     /locus_tag="PC523_000024"
     CDS             22222..23118
                     /locus_tag="PC523_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2473598.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDEDLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            23188..23985
                     /locus_tag="PC523_000025"
     CDS             23188..23985
                     /locus_tag="PC523_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473599.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITYGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(24040..25392)
                     /locus_tag="PC523_000026"
     CDS             complement(24040..25392)
                     /locus_tag="PC523_000026"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDF2473600.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYQGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            25611..26489
                     /gene="purR"
                     /locus_tag="PC523_000027"
     CDS             25611..26489
                     /gene="purR"
                     /locus_tag="PC523_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDF2473601.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDIIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            26645..26923
                     /gene="spoVG"
                     /locus_tag="PC523_000028"
     CDS             26645..26923
                     /gene="spoVG"
                     /locus_tag="PC523_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HDF2473602.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            27136..28515
                     /gene="glmU"
                     /locus_tag="PC523_000029"
     CDS             27136..28515
                     /gene="glmU"
                     /locus_tag="PC523_000029"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDF2473603.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVIIGNDTIIYPG
                     VMLQGKTKIGSDCIIGMNTSITNSEIGDGTEVKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHNVPDGALAIARERQVIKEGWVEK
                     KNQKDNQSK"
     gene            28609..29559
                     /locus_tag="PC523_000030"
     CDS             28609..29559
                     /locus_tag="PC523_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HDF2473604.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      30398..30483
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            30644..31165
                     /locus_tag="PC523_000031"
     CDS             30644..31165
                     /locus_tag="PC523_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2473605.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSIKSAALSYYSDTNKIPVTPDGQTGLNVLETYMESLPDKADIGGEYKLIKVG
                     NKLVLQIGKDGEGVTLTEAQSAKLLSDIGKDKIYTGVTGDNFGEQLKDTTKIDNKALY
                     IVLIDNTVMDSTK"
     gene            31316..32992
                     /locus_tag="PC523_000032"
     CDS             31316..32992
                     /locus_tag="PC523_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDF2473606.1"
                     /translation="MAKKVRIGDKLVEKGYITEDQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            33004..34212
                     /locus_tag="PC523_000033"
     CDS             33004..34212
                     /locus_tag="PC523_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDF2473607.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTAFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTPIGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILVSAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISKQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            34227..35939
                     /locus_tag="PC523_000034"
     CDS             34227..35939
                     /locus_tag="PC523_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HDF2473608.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHNKDKFYIKNLNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIIHEFDLILDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQLKAKQMKNILK
                     IAPVVILILAIPYLCLIFSNNLANKELENVNAEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGANNKADNSSSNKTTTDK
                     SSNNETKSNTKSDSNGDKTNNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSTYTFSMTLYLKEGV
                     LTNE"
     gene            35932..36840
                     /gene="pilO"
                     /locus_tag="PC523_000035"
     CDS             35932..36840
                     /gene="pilO"
                     /locus_tag="PC523_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HDF2473609.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGVLIKVIILLSILSYGYFGLYPQ
                     YQKYIVTKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVREYEHFYGVSTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTSYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            36867..37433
                     /locus_tag="PC523_000036"
     CDS             36867..37433
                     /locus_tag="PC523_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2473610.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGSNYEYNIETPDNIVKYEVSIETKKN
                     TEVYSVTRIEKNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTDKSIYTVSIF
                     YNESVQEGHKNSNLNNKTYTFDVMSRIG"
     gene            37469..37996
                     /locus_tag="PC523_000037"
     CDS             37469..37996
                     /locus_tag="PC523_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDF2473611.1"
                     /translation="MSKKNSKRGFSLIEVLVAMAIMGIILFAFFNIINTNNKANIKND
                     TDINSLNYVQSEIENLREKIKSGEFDFDSLDKMEDGTVVYEKLIDKSKKIVYDKVLSE
                     GNVSLYDTPYEKITTIKDEDGNLIDKENITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            38017..39555
                     /locus_tag="PC523_000038"
     CDS             38017..39555
                     /locus_tag="PC523_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473612.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLMFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQQKSISRSKLRNISDE
                     DFNNQNEENLEVERLGYMNNINFYLNKASSDLEKASIELKKLYDLDMLDYRDIEYVDE
                     NIISHRNSILDICKNYTSGDISKINEYILDEDINSTTLVEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSYTYKALDKISLAMQNMIEYRHTFHIDEQKVEVSNGIPNSQQYYELIQ
                     NPIINSKEYSWNSKWDTLENLLETLPNQTQGFNSLRVHLRNNVRKFEELSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEVDKTEDITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDSKKNIIGLGVEENSNSKYKVEAIINFNLNIDTNIVGNYDIKDKILINHDISSY
                     KKVN"
     gene            39655..40737
                     /locus_tag="PC523_000039"
     CDS             39655..40737
                     /locus_tag="PC523_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDF2473613.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTP
                     EVTMQMAKDIAGESMFKVIKEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPENIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKSGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            40718..41500
                     /locus_tag="PC523_000040"
     CDS             40718..41500
                     /locus_tag="PC523_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDF2473614.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLISGRKCRYCYEKISSRYAIVELITGILFLIVFMLYGISTSTV
                     YYLVLVSLLIIITFIDIDHFIIPDEILIFGAIFAIIFNLLFKEIPVKDSIIGFFICGG
                     SVWLIVLLIEFVIKKECMGGGDIKLFAMLGLYMGVKNSLLTALLSVYVGAVYGICVII
                     ISKTKGKEYNSIIPYGPFISVGALITILCGKQLLEFYFSMIT"
     gene            41602..42252
                     /locus_tag="PC523_000041"
     CDS             41602..42252
                     /locus_tag="PC523_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDF2473615.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLVFCNI
                     VQFDYINHIFGLVIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDIDIKKQIPFTPFISLATILIMFTEYNIFNFYYSLLYKFI"
     gene            42266..42826
                     /locus_tag="PC523_000042"
     CDS             42266..42826
                     /locus_tag="PC523_000042"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDF2473616.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEVGQDLADFVLSRFRKEESDNINE
                     ALEKAAYAIDSIIRENIDMSMNKYNG"
     gene            42843..46229
                     /gene="mfd"
                     /locus_tag="PC523_000043"
     CDS             42843..46229
                     /gene="mfd"
                     /locus_tag="PC523_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDF2473617.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYIEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDNLDLERLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTTNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            46260..47255
                     /locus_tag="PC523_000044"
     CDS             46260..47255
                     /locus_tag="PC523_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2473618.1"
                     /translation="MKKVITLVIAMILVVSVTACNSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFTKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSAKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDASASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            47378..47917
                     /gene="spoVT"
                     /locus_tag="PC523_000045"
     CDS             47378..47917
                     /gene="spoVT"
                     /locus_tag="PC523_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HDF2473619.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            48037..49650
                     /locus_tag="PC523_000046"
     CDS             48037..49650
                     /locus_tag="PC523_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2473620.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNIMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLMFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICIGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            49757..51205
                     /gene="mazG"
                     /locus_tag="PC523_000047"
     CDS             49757..51205
                     /gene="mazG"
                     /locus_tag="PC523_000047"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDF2473621.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLKNSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEIVPSMSFVDAMFNYLAIDPSEGFKLVDAFEIDNSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEYYDYNQEVCIVNGAGVKDLENKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     EFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            51294..51572
                     /locus_tag="PC523_000048"
     CDS             51294..51572
                     /locus_tag="PC523_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDF2473622.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            51644..51886
                     /locus_tag="PC523_000049"
     CDS             51644..51886
                     /locus_tag="PC523_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDF2473623.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            51948..52202
                     /locus_tag="PC523_000050"
     CDS             51948..52202
                     /locus_tag="PC523_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HDF2473624.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            52203..52736
                     /locus_tag="PC523_000051"
     CDS             52203..52736
                     /locus_tag="PC523_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473625.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKSKN
                     KKKSKPKKKKNKTKKRV"
     gene            52742..53026
                     /locus_tag="PC523_000052"
     CDS             52742..53026
                     /locus_tag="PC523_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDF2473626.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINALKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            53139..54059
                     /locus_tag="PC523_000053"
     CDS             53139..54059
                     /locus_tag="PC523_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HDF2473627.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSNKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQDDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDIIEDEELEAMSSFIYDEIDKTLNHIKYRGLKTLIGIGGTITSLSAMNQ
                     ELEVYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            54149..56521
                     /gene="spoIIE"
                     /locus_tag="PC523_000054"
     CDS             54149..56521
                     /gene="spoIIE"
                     /locus_tag="PC523_000054"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HDF2473628.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISIGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAIYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            56872..58764
                     /gene="pepF"
                     /locus_tag="PC523_000055"
     CDS             56872..58764
                     /gene="pepF"
                     /locus_tag="PC523_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDF2473629.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNGDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDEQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMSDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNGNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            58878..59843
                     /gene="galU"
                     /locus_tag="PC523_000056"
     CDS             58878..59843
                     /gene="galU"
                     /locus_tag="PC523_000056"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDF2473630.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEIVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEIRELEIQK"
     gene            60343..61947
                     /gene="rho"
                     /locus_tag="PC523_000057"
     CDS             60343..61947
                     /gene="rho"
                     /locus_tag="PC523_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDF2473631.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKINEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQENRNQRNDYNKTTSVNDCNKPSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            62100..62300
                     /gene="rpmE"
                     /locus_tag="PC523_000058"
     CDS             62100..62300
                     /gene="rpmE"
                     /locus_tag="PC523_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDF2473632.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            62445..63338
                     /locus_tag="PC523_000059"
     CDS             62445..63338
                     /locus_tag="PC523_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HDF2473633.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            63356..64204
                     /gene="prmC"
                     /locus_tag="PC523_000060"
     CDS             63356..64204
                     /gene="prmC"
                     /locus_tag="PC523_000060"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDF2473634.1"
                     /translation="MTIKDIIIKYSDILKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTEFIGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISEIALEIAKKNAITNEV
                     DEKIKYINSDLFTAISNSNIKFDIIVSNPPYIKKQDIETLHTQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKFGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            64248..65312
                     /gene="prfA"
                     /locus_tag="PC523_000061"
     CDS             64248..65312
                     /gene="prfA"
                     /locus_tag="PC523_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDF2473635.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            65441..66121
                     /locus_tag="PC523_000062"
     CDS             65441..66121
                     /locus_tag="PC523_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDF2473636.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGISGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDMSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            66150..67190
                     /locus_tag="PC523_000063"
     CDS             66150..67190
                     /locus_tag="PC523_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDF2473637.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNIIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVAIKINDMIKSNREKGLKTGVMC
                     TSKNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKSVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            67207..67656
                     /locus_tag="PC523_000064"
     CDS             67207..67656
                     /locus_tag="PC523_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HDF2473638.1"
                     /translation="MNILIVCTGNTCRSPMAEAILKKAIKESGRSVEEYSISSAGIST
                     ANGMSASENSIKALKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILLQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDSYRSTMREIVYSVNEIVKKI"
     gene            67673..68125
                     /gene="rpiB"
                     /locus_tag="PC523_000065"
     CDS             67673..68125
                     /gene="rpiB"
                     /locus_tag="PC523_000065"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDF2473639.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLEGKGIECVDYGTNNATDSVDYP
                     VYGEVVANSVINKDVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNNA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            68161..68790
                     /gene="upp"
                     /locus_tag="PC523_000066"
     CDS             68161..68790
                     /gene="upp"
                     /locus_tag="PC523_000066"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDF2473640.1"
                     /translation="MSKVIETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            69090..69905
                     /locus_tag="PC523_000067"
     CDS             69090..69905
                     /locus_tag="PC523_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDF2473641.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEDVECIFCNTEKEIEEALSQRKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRTITNGRAEKNTGVPG
                     AIKGFSKERVMHSQHDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGARVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            70131..70568
                     /locus_tag="PC523_000068"
     CDS             70131..70568
                     /locus_tag="PC523_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HDF2473642.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            70753..70980
                     /locus_tag="PC523_000069"
     CDS             70753..70980
                     /locus_tag="PC523_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HDF2473643.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            70985..71371
                     /locus_tag="PC523_000070"
     CDS             70985..71371
                     /locus_tag="PC523_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDF2473644.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAIRLFIMAVVLFASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            71374..72078
                     /gene="atpB"
                     /locus_tag="PC523_000071"
     CDS             71374..72078
                     /gene="atpB"
                     /locus_tag="PC523_000071"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDF2473645.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGQILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            72147..72407
                     /gene="atpE"
                     /locus_tag="PC523_000072"
     CDS             72147..72407
                     /gene="atpE"
                     /locus_tag="PC523_000072"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDF2473646.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            72530..73027
                     /gene="atpF"
                     /locus_tag="PC523_000073"
     CDS             72530..73027
                     /gene="atpF"
                     /locus_tag="PC523_000073"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDF2473647.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKNDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQEKQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            73024..73569
                     /locus_tag="PC523_000074"
     CDS             73024..73569
                     /locus_tag="PC523_000074"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDF2473648.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIAGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            73586..75088
                     /locus_tag="PC523_000075"
     CDS             73586..75088
                     /locus_tag="PC523_000075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDF2473649.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            75116..75994
                     /gene="atpG"
                     /locus_tag="PC523_000076"
     CDS             75116..75994
                     /gene="atpG"
                     /locus_tag="PC523_000076"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HDF2473650.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIVKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMEVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            76007..77401
                     /gene="atpD"
                     /locus_tag="PC523_000077"
     CDS             76007..77401
                     /gene="atpD"
                     /locus_tag="PC523_000077"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDF2473651.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVKKAKGSM"
     gene            77404..77664
                     /gene="atpC"
                     /locus_tag="PC523_000078"
     CDS             77404..77664
                     /gene="atpC"
                     /locus_tag="PC523_000078"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HDF2473652.1"
                     /translation="MASEFSLKVVTPNNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFVNVTQDKTVILTESAEWI"
     gene            78117..78497
                     /locus_tag="PC523_000079"
     CDS             78117..78497
                     /locus_tag="PC523_000079"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDF2473653.1"
                     /translation="MNIFDIGVDIIEIDRIRKAIEKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRVFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            78512..78973
                     /locus_tag="PC523_000080"
     CDS             78512..78973
                     /locus_tag="PC523_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDF2473654.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            78994..79584
                     /locus_tag="PC523_000081"
     CDS             78994..79584
                     /locus_tag="PC523_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473655.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDIVELPNTGKNNQYLFIGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            79599..80756
                     /gene="alr"
                     /locus_tag="PC523_000082"
     CDS             79599..80756
                     /gene="alr"
                     /locus_tag="PC523_000082"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDF2473656.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKVCGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIVTIPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            80789..81076
                     /locus_tag="PC523_000083"
     CDS             80789..81076
                     /locus_tag="PC523_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HDF2473657.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            81045..81428
                     /locus_tag="PC523_000084"
     CDS             81045..81428
                     /locus_tag="PC523_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDF2473658.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            81599..82426
                     /locus_tag="PC523_000085"
     CDS             81599..82426
                     /locus_tag="PC523_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDF2473659.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            82419..83360
                     /locus_tag="PC523_000086"
     CDS             82419..83360
                     /locus_tag="PC523_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDF2473660.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKAF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNVIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIVKTVKEAIIAKRM"
     gene            83782..84876
                     /locus_tag="PC523_000087"
     CDS             83782..84876
                     /locus_tag="PC523_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473661.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDRLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPIIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDNHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            85102..85518
                     /locus_tag="PC523_000088"
     CDS             85102..85518
                     /locus_tag="PC523_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473662.1"
                     /translation="MDVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(85700..86269)
                     /locus_tag="PC523_000089"
     CDS             complement(85700..86269)
                     /locus_tag="PC523_000089"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDF2473663.1"
                     /translation="MKKEFRKKVIESRKNKNANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLDFNNEVQTDNLIKKLLSLKKIVSSPITIKENRTLIPTQITDLKDGLRIGAYGIR
                     EPNEKSPKIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFNSIPKETHDAQLDYIVTETRIITPNN"
     gene            86423..87580
                     /locus_tag="PC523_000090"
     CDS             86423..87580
                     /locus_tag="PC523_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HDF2473664.1"
                     /translation="MYLVISKKKAIFLGVILIIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGASKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIVEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTGAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            complement(87762..88916)
                     /gene="nagA"
                     /locus_tag="PC523_000091"
     CDS             complement(87762..88916)
                     /gene="nagA"
                     /locus_tag="PC523_000091"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDF2473665.1"
                     /translation="MKSFIYADKFFMKHGTKEKGYLSIINGKFGEFQQERPSTTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNKVEGAKIQGIYLEGPFFTEKYKGAQNEKYFQDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTKYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAIKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLSSGSLAGSVLTMDRAVRNMVDWNISTLEDAIKM
                     ATYVPALSCNIDNVCGSIYQGMDADFIVTDKGFNIHETYINGICKYKVNK"
     gene            complement(88999..89724)
                     /locus_tag="PC523_000092"
     CDS             complement(88999..89724)
                     /locus_tag="PC523_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2473666.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPSTK
                     VLSFEVVESTKYFSEKLGITPGEKIIKIMRLRLANDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEVIKVADEEFSAGIISDKEATLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            89994..90923
                     /gene="pfkB"
                     /locus_tag="PC523_000093"
     CDS             89994..90923
                     /gene="pfkB"
                     /locus_tag="PC523_000093"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDF2473667.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAIGFLGGFNGEFIKNELKKLEIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGINIESRDEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNKDSVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLREIDKYQDLVEVKKLS"
     gene            complement(91052..91969)
                     /locus_tag="PC523_000094"
     CDS             complement(91052..91969)
                     /locus_tag="PC523_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2473668.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     ICSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVNQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKHFSINTSMHRDSG
                     TKLEQVEILSKGRIQRIKNLNVLEIEEGGNLILKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAISGEECVKAQRLLEKIINSVK"
     gene            92441..93610
                     /locus_tag="PC523_000095"
     CDS             92441..93610
                     /locus_tag="PC523_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2473669.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEEL
                     RRFIDKISNNHGQFRVIFTGAGTSAYVGNSILPYLKNNNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDVPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPQGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKIATVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            93659..94510
                     /gene="gatY"
                     /locus_tag="PC523_000096"
     CDS             93659..94510
                     /gene="gatY"
                     /locus_tag="PC523_000096"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDF2473670.1"
                     /translation="MALVTTTSMLKKAQDGGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVKIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLNEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNKV"
     gene            94839..96740
                     /locus_tag="PC523_000097"
     CDS             94839..96740
                     /locus_tag="PC523_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2473671.1"
                     /translation="MRERIILEALKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFKGNRNITKYRNKFIDNIKLDNIRSTIKNVLNEHYLEANLTELEEILAQIIILYIRV
                     STRVVRSPSDLELYTEDLIIDELLDKIFGDKKYSLTYDEKYYLKQLIKLRTKDKKATI
                     KNIDKNKLQDIMCEFFRDIDKKYNSNFLEDKEFLNLFYLHIACLIERIKKNHKITNPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIKNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESFRFVDHIGNVSSPKQYILGLFDKNHFRCVKEKTKYK
                     DILDSMSQCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDINYENRVPKIIFMISLIKGNLELHKQISKYLSKIMTDKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            96743..97786
                     /gene="ypdE"
                     /locus_tag="PC523_000098"
     CDS             96743..97786
                     /gene="ypdE"
                     /locus_tag="PC523_000098"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2473672.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELRAYSDKVICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLMTIGGVKPLAQFVQKVRITT
                     KEGKKIHAIINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTNFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELKKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMKIASENNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEKYRHIRNFI"
     gene            97801..98106
                     /locus_tag="PC523_000099"
     CDS             97801..98106
                     /locus_tag="PC523_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2473673.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            98203..98538
                     /locus_tag="PC523_000100"
     CDS             98203..98538
                     /locus_tag="PC523_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2473674.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            98570..99892
                     /locus_tag="PC523_000101"
     CDS             98570..99892
                     /locus_tag="PC523_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2473675.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGEDNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKIFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPIFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLTVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            99902..100972
                     /locus_tag="PC523_000102"
     CDS             99902..100972
                     /locus_tag="PC523_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2473676.1"
                     /translation="MNNTIERLVSLIDKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQVLDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAISI
                     QEYLSLKNAGFKINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVAREIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            100977..102164
                     /locus_tag="PC523_000103"
     CDS             100977..102164
                     /locus_tag="PC523_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2473677.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKEAILKRASIGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVLNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDLKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETHIPKANVMKSDSSFLAWIDVR
                     GVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(102361..103233)
                     /locus_tag="PC523_000104"
     CDS             complement(102361..103233)
                     /locus_tag="PC523_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDF2473678.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWNGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVSYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPSCEQNDFSKHYL
                     EFKSRNDVEKINLSGSFTDDIISIGKLKNLQLNPELFKNDALIYTNIDEVSICNNNGS
                     KYVTISMKDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADNINSNKIYKDKKFINKLS
                     KGKVFTASYSININ"
     gene            complement(103627..103818)
                     /locus_tag="PC523_000105"
     CDS             complement(103627..103818)
                     /locus_tag="PC523_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473679.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            104038..105084
                     /gene="cobT"
                     /locus_tag="PC523_000106"
     CDS             104038..105084
                     /gene="cobT"
                     /locus_tag="PC523_000106"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDF2473680.1"
                     /translation="MSLLESISKNIYSLDSSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFNIIEASNYT
                     YKNMATFDEIDMGR"
     gene            105116..105676
                     /gene="cobU"
                     /locus_tag="PC523_000107"
     CDS             105116..105676
                     /gene="cobU"
                     /locus_tag="PC523_000107"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDF2473681.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCLDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFKHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIAG
                     RINQYIASKSNEVYFVVSGIPMKIKE"
     gene            105686..106459
                     /gene="cobS"
                     /locus_tag="PC523_000108"
     CDS             105686..106459
                     /gene="cobS"
                     /locus_tag="PC523_000108"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDF2473682.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLVGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRNKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKIGFIYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSTIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSEVVYLIYIYLLNFMFF"
     gene            106479..107093
                     /locus_tag="PC523_000109"
     CDS             106479..107093
                     /locus_tag="PC523_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDF2473683.1"
                     /translation="MIRLILIRHALTNDNKKGKLSGHINSCISEEGKLQINKITRYLS
                     NENIDKIYTTPSTRTKDTIEKISKLKLLEIEEKETLREISFGDFEGITFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLGSKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLDVDSGFTVIDKMNFTDFI"
     regulatory      107160..107339
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            107469..108968
                     /locus_tag="PC523_000110"
     CDS             107469..108968
                     /locus_tag="PC523_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HDF2473684.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKIKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            109007..110383
                     /locus_tag="PC523_000111"
     CDS             109007..110383
                     /locus_tag="PC523_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HDF2473685.1"
                     /translation="MKKILIAGTSSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYRELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDESVPNADYIYIGGGFPEIFGKELEANESMRVSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLNKGNE"
     gene            110384..111355
                     /locus_tag="PC523_000112"
     CDS             110384..111355
                     /locus_tag="PC523_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDF2473686.1"
                     /translation="MNILSIYIGYVTDLIIGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKLGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLSYDHRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            111387..112451
                     /locus_tag="PC523_000113"
     CDS             111387..112451
                     /locus_tag="PC523_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2473687.1"
                     /translation="MKDLGHGANVDEMARLYGKDPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRKNISKYIDINPDFIIPGNGATEVIYLLMKSIKKRLAIINP
                     TFSEYRRSAKLNNLDIIDLELESNFKLNIDIIKENIERFDSLFICNPNNPSGNVQDLK
                     ELVNLLGKHNKVLIIDETFMEFVEDESKHSLVKYIESNKNIFIIKAVTKFFGMPGLRL
                     GYGLTSNAEIMNKIYEYKEPWTINSFADILSNFIFEDREYIKDSKEYYIKERSYMLQE
                     LRNIRNIKVYDTDANFILIRTYKKTAKELKKDLFKQGNILVRDASNFIGLDDSFIRVA
                     IKSHKDNKILIENMKNLLGD"
     gene            112453..113340
                     /locus_tag="PC523_000114"
     CDS             112453..113340
                     /locus_tag="PC523_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDF2473688.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKDTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVIILEPN
                     KVLDTMKIRLRQDYSRLKTENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     DKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKFMNDKNMIDALCESNINSVYSK
                     IYTLNIINGGIKGEIEWNT"
     gene            113328..113960
                     /locus_tag="PC523_000115"
     CDS             113328..113960
                     /locus_tag="PC523_000115"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HDF2473689.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVASIIDALRNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKERGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAEESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            113969..115195
                     /locus_tag="PC523_000116"
     CDS             113969..115195
                     /locus_tag="PC523_000116"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HDF2473690.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLSLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVSKNDKNKEY
                     LTLKDIDGLSNNSESQSELYKFIRVYGGIGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRNLIGDNFESILGNNKVLKITIFAPQGEAVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRTSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKISAGIFNTHSKVADARSEILVANLALMGATYEFLN
                     KINQCLTTEEAVELINNSEYRQVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            115192..115800
                     /locus_tag="PC523_000117"
     CDS             115192..115800
                     /locus_tag="PC523_000117"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HDF2473691.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYISKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREVIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            115790..116365
                     /locus_tag="PC523_000118"
     CDS             115790..116365
                     /locus_tag="PC523_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HDF2473692.1"
                     /translation="MRNSEFVTGKVPITKEEVRAISISKLNLVNAKNFIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNIRKFNLKNIEVIKGYAPINLGLNTKVESI
                     FLGGTGNNLEEIIEWSKKNLVVGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            116358..117110
                     /locus_tag="PC523_000119"
     CDS             116358..117110
                     /locus_tag="PC523_000119"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HDF2473693.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSDADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSRLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            117094..118215
                     /gene="cbiG"
                     /locus_tag="PC523_000120"
     CDS             117094..118215
                     /gene="cbiG"
                     /locus_tag="PC523_000120"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HDF2473694.1"
                     /translation="MNIEGNIAIICITENGKKLAVKINSLVNNCVIYQIKNKKSNLNV
                     EETCVHTVQKKLSDFVGEIFNKFDYIVFIMATGIVVRSIAPYIISKFSDPAVLVMDEK
                     GENVISLLSGHIGGANSLTQYISSLIDANPVITTATDVNQKASLDMIAKKLNAYIDTF
                     RESVKEVNSMLVNNKCVGLYIDEGYQVDTRGFKLLESKDELRDSFSNIYIEKLVVVSN
                     KINCIEDIENLNLDNKFELYYSTEKIIKVIPKDIVIGIGCRRNTDSELMYNSLKEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKTLKVPFKTFSIDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            118212..118937
                     /gene="cobJ"
                     /locus_tag="PC523_000121"
     CDS             118212..118937
                     /gene="cobJ"
                     /locus_tag="PC523_000121"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HDF2473695.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     DKEIIQNGMRQEVDRCKQAVAEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKDGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYIDGELMITPRGYTV"
     gene            118934..119686
                     /gene="cobK"
                     /locus_tag="PC523_000122"
     CDS             118934..119686
                     /gene="cobK"
                     /locus_tag="PC523_000122"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HDF2473696.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAN
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKNAITCAKLSNIDYIRFERKSL
                     IDEIDYDNKFIVNTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIIAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            119705..121183
                     /locus_tag="PC523_000123"
     CDS             119705..121183
                     /locus_tag="PC523_000123"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HDF2473697.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSYDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESDEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGVNAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEEDGFNVRVHLKGLGENSYIQDKFVRHALKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            121213..122118
                     /gene="hemC"
                     /locus_tag="PC523_000124"
     CDS             121213..122118
                     /gene="hemC"
                     /locus_tag="PC523_000124"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDF2473698.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLYGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPRGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSKIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            122108..123607
                     /gene="cobA"
                     /locus_tag="PC523_000125"
     CDS             122108..123607
                     /gene="cobA"
                     /locus_tag="PC523_000125"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDF2473699.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSNYLR
                     EAKPDCEFIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLNKISENLMKEGKDKNTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVELREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNIKLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELMPILNNSDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEDKKDEILDILNSNNIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTDTINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            123624..124595
                     /gene="hemB"
                     /locus_tag="PC523_000126"
     CDS             123624..124595
                     /gene="hemB"
                     /locus_tag="PC523_000126"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDF2473700.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEEEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINADMNVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGANMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKNKF"
     gene            124738..125316
                     /locus_tag="PC523_000127"
     CDS             124738..125316
                     /locus_tag="PC523_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HDF2473701.1"
                     /translation="MLYPINLKLDDLDVVIIGGGEVAYRKCKNFLNFNKRVTIVSKQL
                     LNKFYDLKEYINIIEDDYKEVYIEKASIVIAATNNKVLNMEIGLYCRAEHKLVNVVDN
                     VEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEEIYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLITLDLEELKKFDI"
BASE COUNT        50157 a        13398 c        22799 g        38997 t
ORIGIN      
        1 atttttttat tttttgtcaa aaaaaacaaa aaatacgtat ttcttttttc atttcgggaa
       61 aactaaacac aggaattatg gactttataa aaatttaatt ctcgaggagt gatataagtg
      121 aaaaaagttg ctttaatttt agctataata ggtgcgctta actggggtgc aattggtatt
      181 ctaggcacag acttaatagg tagtatcttt ggtggaacat atgaaatgat aagtagaata
      241 atatacttca ttgtaggttt agctggatta tacttaatac caagtttaat gtctgatgat
      301 agagaataat ttatgctttt atgttaaata cctcattcta aatatttaga atgaggtatt
      361 taattttata aaaatattaa tttgtttctt ttttaatggt aaaatagata agaattgaag
      421 ttttaaaaag aagtattatc taaaaaagga gtactattaa tatatgttta ttattaatga
      481 attaaataag attgtaaatg aagaagtaat atcttttcat atgcctggtc ataaaagagg
      541 agaggtttat aaaaagttgg gatataaaga tatactagag aatttgtata aaatggatac
      601 aacagagata ttaggaacag ataatttaca ttttccagaa gggattataa aaaattcaca
      661 agaaaaagca tcaaaggtat ttaaaagtga ttatacttac tatctagtaa atggtagtag
      721 ctgtggaata caatctgcaa taatgtcagt atgcagtcca aaaagtaaaa taatagcaaa
      781 tagagattgt caccagtctg ttataaatgg atgtattttg ggagatatag atatagagta
      841 tgtaccttgt gagataagtg aagatacaaa tgttttaaaa ggtatatcta taagtaatgt
      901 gattgatata atagataaaa acttggatgc aaaagcggta ctgcttactt atcctacata
      961 ttatgggatg acatatgatt tagaatacat atgtaattat gcacatagca aaaatatggt
     1021 agtgatagta gatgaagcac atggtgcaca tttagggctt agtgaaagac ttcctaaaac
     1081 ggctcttgag caaggtgcag atatagttgt acagagtaca cataaaactc ttccatcatt
     1141 tacacaatcg tcaatgatac atatacaggg aaaccgagtt aatatagata aaatattaaa
     1201 catgttaaga atgacagaat cttcaagccc ttcatactta ttattatctt ctctggaatt
     1261 agcagtagat atatatgaaa ataaaggaaa aggattaatg gaagaacttt taaataatat
     1321 tagaactttc aaaaataata ttgattcaaa tattaagatg tatgacacaa atgataaaac
     1381 aaaaatattt atatcatcta aaaatatggg tttaacaggt tatgaattag aaaatatttt
     1441 aagaaacaaa tataacatac aagtggaatt atctaattat tatggagttc ttttaatatg
     1501 cacaatagga aatactacta aagatttctc tagtttagaa acagcattaa acaatgttgt
     1561 aaaggaagaa tttaaattta caaagctaga aaataaaaaa taccctgttg ttattccgaa
     1621 gaaaatatta acacctagag aagcatttta taaaactaaa aaaagtgtta aaatatatga
     1681 cagcataggt aaaatttgtg gtgaatgtat agtgccatac ccacctggaa taagtgtaat
     1741 atctcctggt gaagtaataa gtaaagaaat aatagactac ctaatgtttt gccattctaa
     1801 aggtatgatt gtgagtggtt tgaaagatgt atatttaaat tttatacaag taatagattt
     1861 agaatacatt taaaacttat gatataaaag tagatagttt aaaatttcga agagaaataa
     1921 atagactgtt tatatagagc tatataataa gagtattaaa aaagtaaaaa gtatttgtat
     1981 ggaggatatg aaaatgaaag gtaagctaat aattatagag agtggttcag atgcaagtgg
     2041 aaaggctact cagacaaaaa tattatatga aagattaaaa aaagaaggct atatgattaa
     2101 aaaggtagaa tatccaaatt atgaatctcc atcatctgca ttggtgaaaa tgtatttaaa
     2161 aggagacttt ggtaaaaatg catcagatgt agacccatat atagcatcaa ctttttttac
     2221 agctgatagg tatgcatcat ttaaaaccga ttgggaggag ttttacaatg aaggtggaat
     2281 agttatcgca gatagatata caacctctaa tatggtacat caggcctcta aaatggatgt
     2341 ttgtgataga gaaaagtatt taaattggtt ggctgattat gaatttaatt tatttaagat
     2401 accacaacct gattgtgttg tatttttaga cgtgcctatt gaatttagta aaaagcttat
     2461 ggagaataga aaaaataaaa ttacagggga agaaaaaaag gatattcatg aaagtgacat
     2521 agagtattta cagaaatctt acaacaatgc tttatatata gcagataaat atgattggaa
     2581 aaaaataaat tgtgttttag ataacaaatt aagaagtatt gaaagtatac ataatgaaat
     2641 ttataaaata gttcttgatt caataaaatc tatggagaga taaatatgta ttttgaaaat
     2701 attatagggc aagattttgc aaagaaatat ttaacaaatt ctataaagaa aaataaactt
     2761 aataatgcgt atatgtttga gggaatggat ggaataggaa aaaagaaatt tgctgatgag
     2821 ttatcaaaac tattacttga ttatgaaaat ctcgaaaata gtccagacta tgtactcata
     2881 aagccagatg gaaatagcat aaaaatagct caaatcagaa atttacaatc agatatagta
     2941 ataagacctc atagagacta taaaatatat ataattaata atgctgaaaa aatgactctg
     3001 gaagctcaaa atgcactttt aaagacgctg gaggaacctc caaactatgc tataataatc
     3061 ttagtcacaa acaacaagga atctttatta gaaactataa aatctagatg tgatataata
     3121 aagttctcac ctataccaat tgaggactta aaaagttatt tgatagatac aggtatagaa
     3181 gaagaaagag ctcaattatt ggctactttt tcgagaggaa gtattgagaa tgctctaaat
     3241 ctatctcagt cttctgagtt ttcagttatg agagaagatg tacaacagta tattcagata
     3301 atgttagata aaaacataat agagattcta aatataccaa ataatatgga aaaatataga
     3361 ggtaagatta tcactttatt agatatgatg ataaactatt ttcgagatat tattctatta
     3421 aaagaaaatg taaacaagaa catgttaata aatgtagata agctggtctt cattcaaaat
     3481 atgagtggaa aaattagtta ttctcaattg tctaagatta ttgatataat agaagatgta
     3541 aagaataaaa taaaaagcaa ttgcaatttc aatataagca tacaagttat gtctttaaat
     3601 atatacgagg tgattaaatg ataaagatag taggtataag gtttaaaagt gctgggaaaa
     3661 tatattattt tgaccctgtt gattttaata tagagcaaga tatggatgta gttgtagaaa
     3721 ctgcaagagg cttggagtat gggaaagtag tcgttggtcc aaaagatatg gatgaaagcg
     3781 aattagtttc tcctttgaag ccaattataa gaatagctac agaagaagat aaaaaagtat
     3841 ttctagaaaa taaggaaaag gcaaaagaaa cttttgagtt atgtcaacaa aaaataaaag
     3901 aacatgagtt aacaatgttt ttaatagatt gtgagtatac atttgataga aataagttga
     3961 ttttttactt tacagcagaa ggaagaatag attttagaga attggtaaaa gatttagctg
     4021 ctatatttaa aactagaata gaacttagac aaataggtgt cagagatgaa gctaaatcta
     4081 taggaggtct aggtccttgt ggtagaaaac tttgttgttc atcttggtta ggtgattttc
     4141 aaccagtttc aataaaaatg gcaaaagacc aaagtttatc tctaaatcca acaaaaatat
     4201 ctggaatatg tgggagatta ttctgttgct taaagtatga gcatgatgta tatagtgagg
     4261 caatagaaaa aatgcctgtt gtaggtgcat tggtgcaatc agatgatggg aaaggaaaag
     4321 ttgtagaagt aaatccttta ttagaacaaa ttaaagtaga atttcaagat aagacagtga
     4381 agatatatac aagagaagat ataaaaatac ttagagagcc taaaaaatgt gatggctgtg
     4441 gtggtaaatg taaggataat gatggattag atgaggccac actaagagag ctaaagaaat
     4501 tagaagatta aattcaaggt agggcttttc ctaccttttt taaagcaatt aatgcttaaa
     4561 gttattaata tttgaggaga atttatatat gcaattaaaa gattcagaaa gaatagatga
     4621 tttacaatta aaaggcctta aaattataca agatactaat ggtttttgtt ttggtataga
     4681 tgcagtttta ttagccaatt ttactaaaat aaaaaaagac gctaaagtag tagatttggg
     4741 tactggaact ggcataatac ctattttgat agctggaaaa agtgaagcta aaaaaattat
     4801 tggtgtggaa atacaagaag atgtatatga aatggcaact cgttctgtaa aattaaatgg
     4861 attagaagaa agagttgaga ttataaatga agatataaaa tacatagaca aagtcttaga
     4921 cgtaaatggt taccatgtag tcacatcaaa tcctccatat atgcatattg atggaataaa
     4981 aaatccaaat gataaaaaag caatatcaag acatgaagta aggtgtaatt tggaagatgt
     5041 aattagagct gcatctagat tggttatgtc tagaggaaaa tttctcatga tacatagacc
     5101 aacaagattg gttgatataa taactttagg gagaaaatat agattagagc caaaggttat
     5161 tcaatttgtg catccaagac ctcaaaaagc acctaattta gtcttggttc aatttgtaaa
     5221 agatggaaga ccagagctta aaatattgga cccattgtat gtttatggag aagatgggaa
     5281 ttatacaaaa gaattaaaag caatatataa taatgaagat ataggagagt tgtagatgag
     5341 tggaaaattg tacatatgtc caacaccaat aggaaactta gaagatataa catatagaac
     5401 tttaagaata ttaaaagagg tagacttgat tgcggctgaa gatacaagac atagtataaa
     5461 acttttaaat cattttgaaa tctcaaagcc attaactagt tatcatgaac ataataagga
     5521 ttcaaaggga gaatatttga taaataaact aattgatggt gaaaatatag ctctgataag
     5581 tgatgcaggt atgccaggta tatctgaccc aggagaagag ataataaaac aagctataca
     5641 aaataacata gagatagaag tattaccagg tgcaacagca tttgtaacag ctttagttgg
     5701 ttctggtatg gacacacata aatttgtttt tgagggcttt ttagatagag ataaaaaagt
     5761 taggaaagca caattggaag aattaaagga agaaagtaga actataattt tttatgaatc
     5821 accgcataga ttgaaggata cactaaaaga tatgtttaaa attcttggta ataggaatat
     5881 ttctataaat agagagctta caaaaaaata tcaagagata ataagagaaa atatagaaac
     5941 tacaataaag atatttgaag aaaaagaagt aagaggagag tttgtattaa tagtagaggg
     6001 ttttcatggt gaaaagtcaa agaaagaaaa ttatgatgag cttactgata gggaatatgt
     6061 tttaaaacta attgaaagtg gtatgaacaa aaaagatgca ataaaaatag tttgtaaaga
     6121 tagaaagtta aagaaagatg tggtttataa acaagttcta gatttataaa acctgaatct
     6181 gaggtgattt tatggaaata ggaaaaatta taatagagag tttaaagaga gaagatacac
     6241 ataaaacatt tatagaatta gatgataagc atgaattaga aaaaataatt cctaaagtaa
     6301 gctgtatgaa atctgtaggt gaatgtaaat atcatgtagt aaattgtttt gaacactcta
     6361 taaatgcatt aaaagaatta gaaatagtat taaataataa ggattttttt ccaatacatc
     6421 taagaaaaca tgtttcaaac tatttaaaca catatattga ggaaggaata aataatttac
     6481 atgtattaaa gttaggaaca tttttacatg atataggtaa acctgatagc atgactttag
     6541 atgaaacagg aagagttcat ttcacaaatc atgaaaaaat aggtgctcaa ataattgaca
     6601 atatggggat aaatctgaag ttatcaacag aaacatatag acttataagt aaatatgtaa
     6661 ggtatcatat gatacttttg tctttgtata agaaaaatga tttaagtagg aaagaattaa
     6721 ttaatgtatt taacttagta gatgaggaca ctataggagt aataatgctt ggatatgcag
     6781 atatagtatc tacagtaaaa ttattaggta agagtgaaga agtcagtgta ttaaagacat
     6841 atatggaata tattttaaca aattacttgt ataaaagtaa ttatacacat gtataaattt
     6901 agtcaatata attgagtttt aaatggttaa ttgaagtttg aaatatatag atttcaaaaa
     6961 cataaaagaa gttgtctttg ttgtaaaatt atgaaagaca acttttttta tatcaaaaat
     7021 tgaaatttta ttaaggatac ttaatggtct attaattaat aaaccattag tatttaattt
     7081 atattacaat attgattttg cttgtattgg cgttgatagt ataacagagg tcttagtaga
     7141 accaattttc tttatagtat caatcacatt ttctagctcg tacatatctt ttacaattac
     7201 ttttaaaagt aagcaatcat cacctgttat atggtgacat tctacaattc tagggtcctt
     7261 tgcagctgac tcaataaatt ctgtatatcc attacttgga agagatatat gaataaatgc
     7321 ctttataact ctacctaatg aatctgggtt gacaatagct ttatatcctt ctataacacc
     7381 agactcttct aatcttttga ctctttctga aactgcagga gaagttaaac caactatttt
     7441 tcctaagtcc ttcatagaaa ttctcccatc atcctgtaaa atttctatga ttctgtaatc
     7501 tgtaacatcc ataaataaac agtccccctt aaataaaaat aattaatgct ataaaacttt
     7561 tttagaatct atcatttgtg tttttaaata ccaaagattt ctagataaat caaccttata
     7621 aatgtgtggt tttgaaagtc ttttgtattg ttcccagata gtacttaatt catattgtac
     7681 atgatttttt atttcaagaa cagctttttt attatatttg catatgcctt ttatatacaa
     7741 tggtttaaga agctctttaa ctttgtaatt tgtaaatacc tttgttttcc atgtataagt
     7801 aggatggaag atttctaaag gtttagactc atctataact tcatcatgta acataatcaa
     7861 atctgcctct gccttattgt cttcgttata tattcttata actttcttgt acccaggatt
     7921 atttattttt tctggattct cagaaatctt tattttaggt tcaagaactc ctttttcaca
     7981 agatgcagct aatttataaa ctcctccaag agaaggtgaa tcgaaagatg taatgagctt
     8041 tgtacctact ccccatgagt ttatagtagc tccctctgct ttaagtgatg ttatggtata
     8101 ttcgtctagg tcattagatg cagtgatgct aatatctgaa aagccagcat tatctaactg
     8161 ttttttagct tctttagata aataagctaa atctccagag tcaagtctta tacccatagg
     8221 tttgtatcct ttttcagaaa tatttttgaa tacttttatt gcattaggaa ctccactatt
     8281 taacacatca taggtatcta caagtagtaa gcatttgtct ggataaacat cagcatatgc
     8341 ttggaatgct tcaagttcat tatcaaattt ttgtacccaa ctatgagctt gtgtaccaat
     8401 tataggtata tcaaacattt tccctgctag tacatttgca gttccagcac aaccaccaac
     8461 tacagcagcc ctagctccat atatacctgc atctggacct tgtgcacggc gtaatccaaa
     8521 ttcaaataca gggtctcctt gagctgcaaa gcaaactcta gatgctttag tggcaataag
     8581 ggactgaaaa ttaactattg tcaacaatgc tgtttcaatg agttgagctt gatacaaagg
     8641 tgccttaaca gtaattaatg gctcattagg gaacatgata gttccttctt caacagcata
     8701 tatatctcct gtgaatttaa attcttttaa aaattttaag aacttgtcag aaaataaatt
     8761 taagtttttt aagtacttta aatcttcatc tgagaaatga agattatcta tgtattcaac
     8821 aacctgatca ataccacaaa ctatagtata ccctccatca caagcgtttt ttctaaaaaa
     8881 catatcaaat acaacaatgt cttcatgaat attcttttca aaatagccat ttaacatagt
     8941 aagctgatat aaatcagtaa gaagtgttag atttctcatt atttctctcc ttaattatta
     9001 ttaaatatta aaaattgtaa aaactcccac acttatacta aaacataata tagtacaact
     9061 ttcaagtata aagtattacc taactatcat aaaattattt aaagaggagg atgattataa
     9121 taggaaaaat atggttttat atggtatcga tagggataat aggaagttta atttctaaaa
     9181 atatgggtga attaaataaa gttatattaa gtgaaacatc aaaaggtata gagtttgcaa
     9241 taagcctagc tggtatcatg gcactctgga tggggataat gaatatagca aaagagtcag
     9301 gcttaattga aaagataggt caaaaattaa atcctataat gagaagatta tttccttcta
     9361 tcccaaaagg tcataaggca atgtcttata tagttatgaa tattgcctta aatatgttgg
     9421 gtgctggaaa tggtgcaaca gcatttggtt taaaagcaat gagtgagctt caaactttga
     9481 ataacaagaa agatacagct actaatgata tgattatgtt catggtaatc aatatatcct
     9541 ctatacaaat aattccattt actataatta aattaaggat ggatatggga gctcaaaacc
     9601 caagtgaaat aatttttacg actttatttg caaccataat atctacagta atagcgataa
     9661 taacatgtaa attttttcaa agaagatata gataaagggg atgtagatac atggagatat
     9721 tttcaaactt acttatacca ataataattt tgtacatagt agtttatggt aaatataaaa
     9781 aaatagatgt gtatgatagt tttgtaaaag gagcgattga tggattaaaa gcagcatggg
     9841 atatcctacc atatataata ggtatatttc tagcaatagg aatattcaaa actggtaaag
     9901 gtcttgatat gttagaatgg ctatttactc caattgcaaa tatgatgagt ataccaaagg
     9961 aattaatagg tctaataagt gtaaaacctt tatctggaag tggtgcttta gggatgtata
    10021 gtgaacttgc aaatagagtt ggaataggaa gtttggtaga aaaaatgggt gccacgattg
    10081 taggttcttc tgagactatt ttttacacaa tggcaatata ctatggaagt ctgaagataa
    10141 aaaatactag acatacatta tcatgtgcta tgattagcca tgttgcaggt gttatagcag
    10201 ctgtttttat ttgctatgta atatttgtat aatttgttga caatatggga aaaattttat
    10261 aatataatga ttatatagaa acaaatagta atataaatta caagctatga aaagaagagt
    10321 aaataaaggt tttttctatc agagagctag attaggtgaa agctagtgtc aaaagcttta
    10381 ttgaagatgg tcttggagct ttttatctga gcaagtgtgc tagggtaaaa cggttggaca
    10441 cgttatagtc tactaaggta tctataaaat gatttctaca tttcatgtga aacataaaca
    10501 ttatagatga attagggtgg taccgtgaat aatctcgccc ctatgttaac tcataggagg
    10561 cgatttttta ttatttaaat gaaagaatca tatgattacg aacgataaaa aattaggagg
    10621 taaataatat gagtaaaccg agtttttatg taacaacacc aatatactat ccaagtggag
    10681 gattacacat aggacatact tattcaacag tagctgcaga tacaatagct agattcaagc
    10741 gtttctgtgg atatgatgta aaattcttaa cgggaacaga tgagcatggt gaaaaaattc
    10801 aaaagaaagc catagagcaa ggaatgtcag agatagagta tcttgatgga atgataaaag
    10861 atataaaggc tttatggaat actatggata tatcttatga tgattttata agaactacag
    10921 aaaaaagaca tacagatata atacaaaaga tatttactaa actatatgag caaggtgata
    10981 tatataaagg tgaatatgaa ggtagatact gtactccttg tgagagtttc tggacagaat
    11041 ctcaattact agaaggaaat aaatgccctg actgtggaag agaaacatat ttagtaaaag
    11101 aagaatctta tttctttaga ttatctaaat atgaagatag attaaaagag ttatttaaag
    11161 atgatagctt ctgttttcct gctgctagaa aaaatgaaat ggtagcaaac tttttagaca
    11221 aaggattaga agatttaagc gtaactagaa cgacttttga ctggggtata aaagttcctt
    11281 ttgatgaaaa acatgtaatt tatgtgtggg tagatgcctt atgtaactat ataacagcat
    11341 taggttatat gactgataat gatgaagaat ttaaaaaata ttggcctgcc aatgttcaga
    11401 tagtaggtaa agaaatagtg agattccata caataatatg gccagcactt ttaatggctt
    11461 taggtctaga agttccaaaa caagtatttg ggcatggatg gatactgttt gctgatgata
    11521 aaatgagtaa atcaaaaggt aatgtggttt atccagaacc tataatagaa agatatggaa
    11581 tagacacgct taagtacttt ttattaagag agtttgcatt tgggcaagat ggaagttata
    11641 ctcatagaaa ttttgtaaca agaataaatt atgatttagc aaatgattta ggaaacttaa
    11701 taagtagaac tgtagctatg gttgaaaagt acaataatgg tataatacca acagcaaaag
    11761 tttcaactga ttttgatgct gatttaaaag agcaagctgt atcaactaga gaaaactttg
    11821 aagctgaaat ggataagatg caattccatg aagcattaga gagtgtatgg aaactagtta
    11881 gaagaactaa caaatatata gatgaaacta tgccttgggc actagctaaa gatgaagcta
    11941 agaaagatga gttagacaca gttttatata atttatgtga atctataaga ataatcgcaa
    12001 cactaataaa tcctataatg aatgagactg ctaataaaat atatgaacat ataggaataa
    12061 agggtcaaga tgatataact acatgggaaa gtacaaaaac atttggtctt attggagaaa
    12121 atgtaaaagt atttaaaggt gaacctttat tcccaagatt agatgtagaa aaagaaatag
    12181 aagagttaac taaaatgttc tctggaaaac ctccagtaga agaaaaacct ttagagcata
    12241 aagaagaaat tactattgat gatttagaca aaatagagct tagagtagga aaaatattaa
    12301 gctgtgaaaa acatccaaaa gcaaataaat tattagtatc gcaagttaag attggaccag
    12361 aaacaagaca aatagtatca ggaatagctg aatactataa gccagaagat ttagtaggaa
    12421 aagaggttac agtagtatgt aacttaaaac cagttaaatt aagaggtgta gaatctcaag
    12481 gtatgatatt agcagcaggt gatgatggag acccatatgt tttaccattt acacaaggtg
    12541 ctaaagatgg atgcgaggtt cgttagtagg caattaaaga gtaaggagat taaagatgtt
    12601 atttgactca catgcacatt taaatgatga aagttttgat gaagatagag atgaacttat
    12661 tggttcatta aaagataaag gtgtagattt ggttgtaaat ccaggagcag atatagatac
    12721 gtcgataaca gcaattgaac ttgcgaaaaa atacgacttt atatattcag cagttggagt
    12781 acatccacat gaggtatcaa aactagatga tacagcaata gaaattctaa gaaaacttgc
    12841 aactgagaat gaaaaagttg tagctattgg agagattgga ttagattatt attatgatta
    12901 ttctccaaga gaagaacaaa aagagtggtt taagaagcaa attgaattag ctaatgagct
    12961 aaagcttcca ataataatac atgatagaga tgcacatgga gatacttttg agataataaa
    13021 gaatactaag agtcctgaaa taggatgtgt acttcattgt tatagtggaa atgtagagtt
    13081 agctagagaa tatgttaaga tgggttgtta tatatcaatt ccaggcacgg ttacatttaa
    13141 gaataataaa aaaactagag aagtagtaag agaaatacct ctagaaagat tattcataga
    13201 gactgattca ccatatatgt caccggagcc acatagagga aagagaaata atccttctca
    13261 agtatctttt gttgctgata agatagctca ggaaaaaggt atatcttatg aagaagtatg
    13321 tagagttaca aaagaaaatg caaagaaatt ttttaatata aaatagacaa attaagtcct
    13381 ataatggtat ataaaataag tcattataag tatagaatga gggtgcctca aaatgaattt
    13441 tttagttcat gaggtactct tttttatatg aaatcaaata tattttcgtc atttcaacaa
    13501 tgaaaaataa gtttatccta ttttgataca gtctatttct atttatttaa ttcatatttt
    13561 aaaatatatt tttttaacat tgttaaaaaa gaatttacaa gatattcact agaattaact
    13621 caactataat attcatttaa cttaccttgt ttattatatt attaaagaga agtaaacgaa
    13681 ggacagatta aggaggctaa attttgtata cagttgatag tactaagata ttgataaata
    13741 tgaacttaga gaaactagaa aagatatact caatgttaaa gtatacatca aatgtttcta
    13801 taattaaaga acctaagttg gctacagtaa tggaagagtt aatgaaaatg taaaaaatac
    13861 aacatttaat ttaggggata tattagttac tgaatgtagt gtagaggtag atgaaagttt
    13921 gggttacgga atagttgcag aaaaaactaa taatttaaat aaagcagtaa gtttatttac
    13981 ctcaagcact attaaagttg gtatatatga tgaaagaagt agtgtaaaag aaaataaaaa
    14041 gcagatttag tttcagtaga tattaacagt gaatatccaa agcttgtaag tattattgta
    14101 aatggaaaag cagtttataa aagggaggta taaagattgt taacagagtt acactgtcat
    14161 acaaatatat cagataataa ttttactact gaagaaataa taaaaaaagc agcagaaaag
    14221 aatataaata tcttagcaat aacaaatcat gacacattat ctggattgga tgaagccatt
    14281 gtgcttggag aacattatgg aataacaata ataccaggta tagaaatttc agcttatgat
    14341 tatataaata gaaaaagagt tcatattctt ggatataaca tagatttaaa cagtcaagaa
    14401 ataaaaaatt tgtgcaatcc aatggtatgt gacagacaca aagcatctgt taagatggta
    14461 aataaaatta tagatttagg gtacaaaata tctctggagg atgtaaagaa atattcatca
    14521 aagactggaa tatttaaaca acatataatg aaggctctta tagataaagg atatacagat
    14581 aatatatatt caagtctata taaagaactg tttcatagag gaaacggtaa ggtatatgta
    14641 tctttaagat atgtagatta tagagatgca ataaaagcta taaaaaattc tggtggaatt
    14701 tgtgtgttag ctcatccggg tcaaatggat aattttagcg ctattgaaga gatggtaaaa
    14761 gtaggattag atggaataga agtatatcat ccatctcata acaaagaact agaaaaagaa
    14821 tcgctttctt atgctaagaa gtacaatcta gtggtcacag gaggctcaga ttaccacgga
    14881 ttttattctc aacataatta tgaacttgga agtaagagct taaataaaga agatttattt
    14941 aagtttaggt tagctttagg aggggtatta taaatgtctt ttttacaagt aaaaaatgta
    15001 tgtaaaagtt atggacaagt taaggtgtta aaagatatat ctattgatat tgaaaaagga
    15061 gagtttattt gtttactagg accaagtggt tgtggcaaaa gcacactgct tagaataatt
    15121 gcggggcttg aggataaaca tggtgggaaa ataataataa atgatagaga tatgactaat
    15181 tcaccacctg aaagtagaaa ttttggtata gtttttcaat catatgcatt atttccaaat
    15241 atgaatgttt acaaaaatat agcttttgga ctagaaaata aaaacataag taaatctaac
    15301 attgataaaa aagttaaaaa agttctagaa gttgtagaat taagtggata tgagaaaaaa
    15361 tatccatcac aattatcagg tggacaacaa caaagggttg cattagctcg tgcgattgct
    15421 ttagagccag actttttact tcttgatgaa cctttatctg cattagatgc aaaagtaaga
    15481 cttaaactta gggagcagat tagaagtttg catagaaaac taggcattac aactataatg
    15541 gtaacgcatg accaagaaga agctctttgt ttagctgata agatggttgt tatgaataga
    15601 ggagaaataa tacaagttgg aactccaaag gaagtatata aaaatccaga aacacctttt
    15661 gtagctgatt ttataggaac aataaatttt atagataatg gtattaataa gatagctatt
    15721 agaccagaag acataaaggt agagttcaat agagattcta aagataaaga tataaaagag
    15781 ggagagattt tggatataga atttagagga tttaactata gaattacagt tgagtattgc
    15841 tctaagcaaa tgaagttaga tgtagtttca aaagttgcag aacaaatgaa actgtgtata
    15901 ggttctaaaa taaactttaa gatacctaaa gaaggaattg tacaatataa atcagaggga
    15961 tgtgcttaat agctagggat gaataaatga taacatcaaa aaataaaaga agaataagtg
    16021 atagcacaat acaaaaatat ttcttgattt tttcagtaat tgtactaata gtatttatat
    16081 tatttccgtt agcatcatta ttaaaaaatg catttatgga tacaagtgga aattttatag
    16141 gaattttaaa ctttagtaaa tatattgaga atccaagtct tatcgcatca tttaaaaatt
    16201 caatttttgt atctatagtc tcatctttaa tatcattatg tttagcattt atatattcat
    16261 acgcaataac aagaacaaat ataaagttta aaaacttttt taaaactatg ggaatgtttc
    16321 cactttttgc accaactatg ttatatggta tttcacttat ataccttttt ggaaataagg
    16381 gaatctttac aaccatgggt ctggaagtac cactatatgg tccattagga ataattattt
    16441 ctcagattct atttacattt ccacaagctt ttttaatatt atctgtagca ctttcgatgg
    16501 ctgattacag actttatgaa gcagcagata gcttgggtgc tagtgaattt aagaaatttt
    16561 gcaaaataac aattcctggt ataaagtact cattacttag ttcattcttt gtatccttta
    16621 tacttgcatt tacagatttt ggagcagcta aggtagttgg aggaaactac aatgtactgg
    16681 ctactgacat atataaacaa gtagttggac aatttaacat accaatggga gctactgttt
    16741 ctatggtaat gttaattcct gtactaatag catttacatt agataagatg gcaagtaaga
    16801 aacaaggtat gaccataact gcgaagtcta tgccatataa gattaaagaa aacaagtcta
    16861 gagatagagc gtttttaata gtatgtagtc ttataacgtt gctaattttt attttgatat
    16921 ttgtttcagt tataggttca gttataaaat tatggccata taatttaagc tttacaatgg
    16981 aacactatac atttaaaggt attttgggaa gtggaataaa aacatattta agcagtttga
    17041 aaatatcaac tttaacagca gtatttggaa ctatatttgt attttttagt gcatatatga
    17101 tagaaaaggt ggataagttt cctaagttaa gacaaatagc ttacttttta tcaatgatac
    17161 caatggcctt gcctggctta gttttaggta tatcatatat aacttttttt aactcagcaa
    17221 gtaatccact aaatttttta tatggaagta cagcaatact agttatagta aatatagttc
    17281 atttttattc agtttgtttt ataacttcaa actcatcttt aaaaatgttg gatatagagg
    17341 tataaagtta aaagaggaag gctatccagt agaagtagta tttccaaaag aaggttctgg
    17401 atgagatata gaagcaaatg ctcttgtgaa aaaagataat ataaaagaag cctcaaaagt
    17461 atttttagat tgggcaataa gtaaagatgc aatgaatgaa tattctaaga actatgcagt
    17521 tactactatt tctacgggaa atccaatacc agaaggattt cctaaaaagc cattagagca
    17581 aatgatagat aatgatttaa aatcagcagc gaaaaataga gaagatatac taaataaatg
    17641 gatatcaaaa tatgatggta agacagaaaa agagagttaa gcctgacaat aaaatttaat
    17701 acaaaaagcg cctagaatat aaattttata tctaggctct tttgatgtgt ttgtttaaaa
    17761 tgcatatttc tatataaaaa taaatagttt agtaccttga ttaaattaaa taaaaacctt
    17821 aaaataaaga gatgtaaaga aaaattaaaa acgaataaaa ggtgaattta tatgataaaa
    17881 gaaataatag tagtagaggg tagagatgac gttacagcag ttaagagagc aatagatgca
    17941 gaacttataa ctacaggtgg atttggattt ccaaaaggtg taatggagcg tataaaagct
    18001 gctaatgaaa gacgtggtgt tataatattt acagacccag attttgcagg tgaaaaaata
    18061 agaaaaaaaa tagcatcaga agttccaggt tgtaaacatg catttctgcc aagagaggaa
    18121 gctaaaaagg atggagatat aggtattgaa aatgctactc ctcaaagtat aatagcagct
    18181 ttgaataagg ttagaactga aaatacagaa aaaagagatg aatttaagca aattgactta
    18241 attagaaatg gtcttatagg taatgaagaa gcatcactta gaagagatgc acttggaaaa
    18301 atacttggaa tcggatatgg aaatgcaaaa caatttttaa atagattgaa taattatgga
    18361 gttgaaagag aagcatttat agatgcatta aaacaaatat aaatgtaatg tttaatatat
    18421 gaaaaatatg tatataaaat aaattgtgga attacggtta atttaagata aattatttat
    18481 aatatatacc ttattttaaa tcaatgtaaa tacatataaa taagtataat taataaaatt
    18541 aaatatcaaa ataagttaaa aaggagtaaa taatggatag actatcttca cataatgcaa
    18601 caaaggaagt agtacaaaaa tataatttta agttttcaaa gtctttaggt caaaatttct
    18661 tgatagatag taatataatt gataaaatct tatctggagc tagaatcaca ggaggagata
    18721 atatcataga agtaggacct ggtataggaa ctcttactcg tgagatgggt aaaatagcag
    18781 aaaaagtagt ggctatagaa atcgatagaa atctaatccc tatattgaaa gataccctgt
    18841 cagatttaga gaatacagag gttgtaaatc aagatatact aaaagtagac atacaggaat
    18901 tagtaaaaga taaattaaat ggtggaccag ttaaacttgt agcaaatctt ccatattata
    18961 taactactcc aatagttatg aagtttttag aggaagatat acctgtaaca gacatagtag
    19021 tcatggttca aaaagaagtt gcagatagaa tgaatgctat tccaggtact aaagattatg
    19081 gtgcattatc tatagctgtt caatactatt gtgatacaga gattgtggca aaagccccaa
    19141 gacatatgtt tataccacaa ccaaatgtag attccacggt tataggctta catgtaagag
    19201 ataaaagaaa atatgatgtt cataatgaag aaatattttt taaaacagta aaagcatctt
    19261 ttggacaaag aagaaaaact cttcttaatt cgttgggagg tcttggcttt cttaataaag
    19321 atgaaataag agaaatatta aaagaagcta atatagacga aaaaagaagg ggtgaaacgc
    19381 ttagtataga agagttttca gttctttcaa atataataaa tacaaaagta tcttctaaat
    19441 agaaggtact tttttttatt ttgttaacat atattgttta gagaaactgt gttaaggggg
    19501 gattgcagtg ggttctaaaa gaaagtttaa aagagactat ataatactag aagctaaaga
    19561 cataaacttt agatacaaag aacgagtact gccaaaagct tttgcaaaag ttgaagtaaa
    19621 cgatgaaaaa gcaattattg cattatatgt agaaaatcta aaatatgtta aagatgggta
    19681 taaagcagta gcaataaaag atgaccatgg attaatagac ttaggaaata taattttaaa
    19741 tgaacaagga agaggtgaat ttgttttaga tttagaagat tatgatgatg agataaaagg
    19801 tattgcatta ttgtatgaaa gaaatatacc tttaattgga tttaagggaa ataagcttga
    19861 aaattatgag gatatattat tccaagaggt agaggaagaa gaatatgaag aattggaaga
    19921 agaagacgaa gaggtagagg ataatgagga aagtaataga gacgaagata ttgaagaaga
    19981 agaatatatt atagaagaat atgatgaaga tgagtatgaa gactattggg aagatgatga
    20041 agatattgaa gaaggagaat taaaagcaag aacggaagta gaagattcta gtaatgaaga
    20101 aatagaagag ttcaataatt acaaggaaat agaaaattat aatagggtta aattacagga
    20161 agaatatata gacatagaat gtgaagagga tgaagaagag gatgaagaag aagtttttga
    20221 agtaaataga gaagatgaca ggtttgtttc ttcaaaacct actaaatcaa agaaagttca
    20281 aaaagagcag aataatattg aaaaaataaa aagagtcaat ggagtaagtc aaggagagag
    20341 tggaaataaa caaaccagta cattacttat gccaagacaa ataaaaaaag gtttgaaatt
    20401 atttaaagaa gtaaaacctt ttgtaacaga ttatatagat aagacaagat ggtggaaaat
    20461 agagataaat cctacaacat tatgtggata tacaatgcct tatttaggat atttaaacgt
    20521 gttgaattat actatgtata gtgatgcagt tttacattca tataaatata gacactattt
    20581 atttggagtt cagtatgatg aatataataa gagaaaacat tatatttatg ctatcccagg
    20641 aaatagaaat gaacagccag acaatggaag cacaggattt ttaagatatc aggcttgtga
    20701 taatagaaca aatagtttag ggtattggct atgttttata gattctagac aaagaaatat
    20761 agttaggtaa aaataataaa aataaaaaaa tttagactca atttttattt tactgaaaaa
    20821 attaatgaaa tgcagtacaa aactttaaaa actattgata atatttaaat attacactac
    20881 aatatatctt gaaataccta ttatattatg gaggaaaatt atgataaacg agcaaagaat
    20941 tttaaatgag tttcttgaat tagtacagat agacagttta tctttaaaag agggaaatgt
    21001 tgcgaaagta ttagttaaaa aattagaaga aataggttgt tcagtagtag tagacaatgc
    21061 tggtgaaaaa gcaaatggag aaactggtaa tataatagct acactaaagg gaaataaaga
    21121 aggaaaaaaa atattattta gttcacatat ggatacagta actccaggta taggggtaaa
    21181 acccatagtg gatgaagtaa acggaataat aaaaagtgat ggtactacaa tattaggttc
    21241 tgatgataaa gcaggtatag cagctatatt agaaggatta agatatataa atgaaaataa
    21301 catagaacat actgatatac aagttgtatt ctctatatgt gaggaatgtg gattagttgg
    21361 agctaaaaat ttagactatg gaaaaataga ctctgaatat gcttttatat tagatagtgg
    21421 tggttctcca ggggaaataa tagtaaaagc tcctgcacaa gatgtaataa atgttaagat
    21481 attaggaaag actgctcatg caggtcttga acctgaagct ggtataagtg ctataatggt
    21541 agctgccaga gcaatagaaa atatgaattt acttagaata gatgaagaaa caactgctaa
    21601 cataggaatt ataaacggtg gaacagctac aaatatagta acaggagaag ttaatatagt
    21661 tgcagaagct agaagtttaa aagaaaataa attagatgtt caaactaaac acatggtaga
    21721 gacttttgaa aaagctgcaa aagattttgg agctcaaatt gagatagatg tcaatagagc
    21781 atatacacca atagatgtta gtgaagattc tgaaataata aaacttgcaa agaaggcttt
    21841 ttcaaactta ggtatagaag gtcacactga atctacagga ggaggtagtg atacaaatat
    21901 attaagtaag aatggtatcg aagctataac tttaggaatc ggtatgaaaa atgctcatac
    21961 actaagtgaa catatagcta taaaagattt atatgattct gctaaaatgg tagtggagat
    22021 aataaaggaa gcataaattt aaataatagt gtataatata tatatcgcct agcttaagct
    22081 aggtgatttt tcattttata gaagattgat aatctctatt gaaagcaaag agtatacatt
    22141 tgcatataag agagtgttta atttatattc agatgtaggt tcttaataat atttagattg
    22201 tttcataagg gaggaataaa tatggaaaca aggtatgaag aagcaaataa aataacaata
    22261 caatcaatac tttggaatgt agtattaaca ataattaagg ttatagctgg tgttattggt
    22321 aattctagtg ctatgattgc agatggatta cattcagcat cagatattat aagttctata
    22381 ggagtattaa taggcaacta tgtatcttct agacctggag atagagagca taattatggt
    22441 catgagaaag cagagacatt agtgtcattt gtgttatcaa tactgcttat tttcgtttct
    22501 ataacaatag gtatagaggc aattaaatct ttatttaact tagatgcatt gagtgttcca
    22561 tcaatattgc cattagtagt atcagttatt tcaatcctga taaaagagta tcaatataga
    22621 ataacaataa aagttgctaa aaagataaat tctccagcct taaaagctga tgcttggcat
    22681 cataggtcag atgctttatc ttcagtagct gcttttatag gtataggtgg ttctatttta
    22741 gggtttaagc cattagaccc aattgcatca gttgtagtgg ctatatttgt ggctaaagta
    22801 ggaattagta tacttataag ttctgttaat gaattgatgg atgtttcagt tgatgaagag
    22861 gagataaaag aattaaaatt tattgtagca gatacagaag gtgttaaaaa tcttggagat
    22921 ataaagacta gaaaacatgg agctatggca tatgtagatt taactatatg tgtggatgaa
    22981 gatttgactg ttaagcaagg tcatgatatt gctactaagc ttgaaaaaca tataataaaa
    23041 catatggaat ttgtaaaagg cataacagtt cacgttgaac catgtactaa ttgccaagga
    23101 aataagtgta ataattaatt aaaacttaaa aaaaagtgta aaaagttata taattgtttt
    23161 atgattataa actctagggg tggttaaatg agcaaaaaga gaaaactaaa gaaaaaagct
    23221 gcaatattga taggagtggt tgttttcttt ataatatact ttatatcctt tagaatagga
    23281 ataaatatgg aaaaggataa aagtgaagtg ccaaagaagg tagaaaaaga agaagaagat
    23341 aataggtctt taaggactca aataaaagaa aagaagaagg tttatatatc agataaaaat
    23401 atagaaaaca taaaaattga ggaagatatg tgggatgaaa taaagttatt tttctcagat
    23461 tttaagaaag taagaaatac tacttcttct tatgaagcag tctatgaagg ctattctgat
    23521 gatggagtta aattttctac tgaccttaac ttttttagag tatatactgt aaacaaagaa
    23581 gaatattata aagtgccagt tagttctaag gatgctttta agaaactttt agatgagagt
    23641 atatatactt cgtttgacct tctaagtcat tataaagatt ggaaagaggt tactataact
    23701 tatggtaaag aaattaaaaa agttcataga tggaagtttg atgatttagc acataagatg
    23761 atttcaaaaa gggttgtagg aaaagttcaa cctgaaaaaa gtaaggaaag aagtgactat
    23821 aattttacta taaatataaa aggtgataat tttgatgtaa aagttgatac aatgggaaaa
    23881 gattatgtaa aagttacttc taagaaaaat gtatcttact atgaggttca tacaagttta
    23941 tttgattacc ttaaaaatga aatatttgaa attggaaaga aataatactt taagaaggct
    24001 atcaaattag atatttgata gcctaaattg ttttatttat tatttttcaa ccaacaagtc
    24061 tgcaacctta taaattggac cagcaccagc agttattact aaatcattgt ccttaacatt
    24121 ttcacgtaag tattttacta tatcttcaaa ttcttttata tatatggcat ctacattgtt
    24181 ttgatataat ttctcaacca agtccttaga atgtatatct ccggggtctt tttctctagc
    24241 agcatatata tctgttatga taactttatc agcagcatag aatgcttctg aaaattcacc
    24301 taaaagagat tttgttctag tataagtatg tggctggaag atacaccata aagtagattt
    24361 ttttaatttt ttggcagcag ctaaagtagc ttttaattca gttggatgat gtgcataatc
    24421 atctactact aaagcattct tataataccc tttagtttca aatcttcttc caacgccttt
    24481 ataaattttt atattttttc ttatagtctc taaatcaata ccagaaacaa gagctgccat
    24541 tatagcagaa gaagcattgt atatgttatg aagaccatat acagaaagtt caaattctcc
    24601 taaatctctt ccttgatact ctattctaaa tattccatga ccactattgt caaagtgaat
    24661 atcttttatt acagcatcat tgtcagaatc tctaccgtat tttataatag tagctttaac
    24721 atcatacaat atgtcatcaa tattttcatc atcaccattt attatgaaat agccttctgg
    24781 aggtaataat tttccaaatt tgttaaaaga agctttgatt tcatctatcc cagagaaata
    24841 atcaagatgg tcctcttcaa catttaaaac tatagatatt ttggggttaa aattaaggaa
    24901 actatctaca tattcgcatg cttcagttat aaagtggttg gaattaccta tcttaacatt
    24961 accacctatc atacttaagt tgccaccaac tagtatagtt gggtctaagt ctgcatattc
    25021 aaagattgta gataacatag atgttgtaga agtttttcca tgtgttccag aaactgctat
    25081 agaattttta tattccctca tgatttggcc taaaaaagtc gctctgttca ttactaattt
    25141 gtttttttct ttagcagcca caagttcttc attgtctggg tggactgctg ctgtataaac
    25201 aaccatattt acatcatctg atatattatc ttttttctga ccaatataaa tagttgcccc
    25261 ttggtctcta agtttgtcta gtaggtatga ttcgtttgaa tccgaacctg aaacttgata
    25321 acctttattg atacatattt cagctaatgc actcatgctg ataccgccaa ttcctataaa
    25381 gtgtatattc ataataaaac cacctttctt tttaattaag tcctacaata tgaactattt
    25441 ctgtggtata ataatataat gttcaatatt ttataattaa tatgcatata atattatatg
    25501 tattttattt ttgaatataa taaaacataa gtaatattat accacaataa gaattgttat
    25561 gcattagtta gtttaacgtt atataaaggt acttgtagga ggaaataata atgaaattta
    25621 aaagaacaga gagaattgga gcaatagtta aaatactgtc tgataatcca aataaaatat
    25681 atacattaag ttattttaca aatcaattta atgcagcaaa atcaacaata agtgaagatt
    25741 tattagttgt gaaaaatgta tttgagaagt tacacttagg taaggtcata actatctcag
    25801 gagcagcagg aggagtaaaa tatataccta aaacttctat agcagaaaat gcagagtttt
    25861 taatggagtt atgtgagaga atatgtgaca agtctagaat attatctggt ggatttttat
    25921 acttgattga cataatttat gaccctacaa tagctgctaa gataggaaag atatttgcat
    25981 caaatattga gtatgtagat gcagattatg ttgtgacaat ggaaacaaaa ggtataccta
    26041 tggcacttat gactgcaaaa gctatgaatt tacctctagt tataataaga aaagatataa
    26101 aggtatcaga gggtcctaca cttagcatga catatgtgag tggaaacagc tctaaagttg
    26161 aaagtatgag tttgccaaga aaagctctta aaccagatag taaagttata ataatagatg
    26221 atttcatgag aggtggaggt actataaaag gaatggtaga ccttatgaat gaatttggtg
    26281 cagaagttat aggtactgga gtattcatat ctacaacgaa tccttcagaa aaaatggtaa
    26341 aagattatat ttcattaatt caattggatg ttaatggaga taaaatagta gtagaaccaa
    26401 acttaaagac ttttaaagat gaatatagaa atgaagattt agatgaagaa gaagattcag
    26461 aatttgaatt tgagatagat gaagattaat aaaaaaaata tttagaaaaa tatgtaaaaa
    26521 taaaaagagg atatccctag ttgttcatag aattatttaa aaaacagttt atattaattt
    26581 attccagcga taataatttt aatataaatt aatttctgtc aaaatcaaag ggggatattt
    26641 tggtatgaag ataactgacg taagagtaag aaaattaaca gaagaaggta agatgaaatg
    26701 tatagtttca ataactttcg ataatttgtt tgtagtgcat gatataaaag ttatagaagg
    26761 ccataatggg ctatttatag caatgcctag cagaaaagta ggcgaaggaa atttcagaga
    26821 tatagctcat ccaataaatg cagaaatgag acaagtttta gaagatgcag tattacaagc
    26881 atatcatgaa gcattagttc aatgggaagt agctgctgaa taataataaa tatataaaat
    26941 ggagagagcc cgaacattta ggctcttttt aaatgtagaa tttctattaa aatatgtact
    27001 ttttagtaaa aaagtgctat attattagtg ataatgtata tgttgtatac aaattgttag
    27061 ataaatgtat tataatgtat attgattaag agaataatac gaataaaatt aaattagatt
    27121 gaatggagtg tatcaatgaa ctttaaagct ataatccttg ctgctggcaa aggaacaaga
    27181 atgaagtcta aatacccaaa agttatacat aaagtatgtg gaaaagaaat ggttaaccat
    27241 attatagatg tttcaaaaaa atcaggggta aaagatactg tcgtgatttt agggcatgaa
    27301 gctgatgtag ttaaagaaaa gcttgcagaa gaaattataa tagccatgca aacagaacag
    27361 cttggaacag gacatgctgt gaagatggct aaagaatata ttaatgatga agatacaata
    27421 gttgtacttt gtggagatac tccacttata aaagaagaga ctctgaagag actatttgaa
    27481 tatcatatag aaaataaata tcatgctaca gttcttacaa caagagttgg aaatccaaca
    27541 ggttatggta ggataataag agataagaag ggtgatcttc ttaaaatagt ggaacaaaaa
    27601 gatgcaaatt cagaagaaaa aatgataagt gagataaact caggaatata ttgttttaat
    27661 ggaaagagtc ttagagaagc actagatttg ctaaataata acaattcaca aggtgaatat
    27721 tatttaactg atactgctaa aataatgaga gataaaggat taaaagttgg tgcatttgca
    27781 ggttcaacta ttgaagaact tatgggcgtt aattcaagag tagaattatc aaaggcagaa
    27841 gaaattatga gacgaagaat aaatgaatct catatggtaa atggagtgac tataatagac
    27901 acgaactcta cctatataga atctgatgtt ataataggta atgataccat aatatatcca
    27961 ggtgttatgt tacaaggaaa aacaaagata ggttcagatt gtataattgg aatgaataca
    28021 tctataacaa attcagaaat aggtgatggt acagaagtaa aaaactcaac tataattgat
    28081 agtaaagttg gagaaaattc tactgttgga ccatatgcat atctaagacc aaagagtgac
    28141 ttaggaaata atgtgaaaat aggcgatttt gtagaagtaa aaaatgctat tatagaagat
    28201 ggctcaaaag catctcatct ttcttatata ggagatgcac atgtcggcaa aaatgtaaat
    28261 attggatgtg gagtagtatt tgtaaactat gatggtaaaa ataaatttaa atcaatagta
    28321 aaagacaatg cgtttatagg ttcaaattct aatttagttg caccagtagt tgtagaagaa
    28381 aaaggatata tagcaacagg ttcaacaata acacataatg ttccagatgg tgctttggca
    28441 atagcaagag aaagacaagt tattaaagaa ggttgggtag aaaaaaagaa tcaaaaagac
    28501 aatcaaagta agtagttatg tagtttatta ttttggctaa aaatttagct cgaaattatt
    28561 ataaattata ttcattagta ttttaaaatt ttcaggaggg ttacataaat gaatactagc
    28621 ggaagcgaga ttaaaattat tgcaggtaat tcatcgaaag agttagcaca aaaaatagct
    28681 gactatatag gtgtatcagt actagattgt gaagtaggta catttagtga tggtgaaata
    28741 tgtgtaaaca tgaatgagac agtaagaggt tgtgatgtgt ttgtagtgca atctactaac
    28801 agcccagtaa atgataactt aatggagtta ttaatcttaa ttgatgcatt aaagagagca
    28861 tcagcaggta gaataacagc agttattcct tactatggat atgcaagaca agatagaaag
    28921 gctaaggcaa gagatccaat cactgctaaa ttggttgcaa acttaataac tgctgctgga
    28981 gcagatagag tattaacaat ggatttacat gcagctcaaa tacaaggata ctttgatata
    29041 ccattagacc acttactagg tggaacaata ttagcaaatt attttaatga aaagaaaata
    29101 gaagatttag tagtagtatc tcctgactta ggaagtgtta ctagatcaag aaaatttgct
    29161 aatactttaa atggagaagt tccaatagct ataatagata aaagaagacc aaaagctaat
    29221 gtatgtgaag ttatgaactt aataggtgat gttaaaggta aaaatgttat actattagac
    29281 gatatgatag atacagcagg aacaatagtt aatgctgcaa atgcacttaa agagtttgga
    29341 gcaaaagatg tttatgcttg ttgtactcat ggtgtgttat caggcccagc tattgaaaga
    29401 attgctaatt cagagataag tgagttaata gttcttgata ctatacaact tcctgaagag
    29461 aaaagaatag ataagataaa gataaaaaca gtagctcctt tatttggaga tgctataaga
    29521 atgatattct ctaatgaatc agtaagtaaa ttattctaat ttattgtatt tatgagagtt
    29581 gtttaaaagg atataagcct tttgagcaac tctttttgat ttacttatgg aatgttattt
    29641 ttctcttgtt agaaaggtat ctattattgt agaaagagat tatagtttaa aatttatgta
    29701 tatttaatca aaataaattt aataaaagat atattattca aaatattatg aatataaaaa
    29761 atattacaaa aaagtattga aaaaattcag attatgtagt attatgtaaa tataaataaa
    29821 gaaagaggtg tttccaatgg gaaggttaga agatgaaccc ctaataatta aatgattcag
    29881 tcacaatcta aagtgaatat aaaaatctat tgaaagttta tttataagaa ttgaaaatag
    29941 tatctattct atctttagtg tttgtgagag ggttctggat tatataaaat tactgtaaag
    30001 aaagtaattt tgcacaataa gcatatctgt aaatgaagag tttcgtttat ataatgattg
    30061 tggctgaatc ataactataa tagatagaat gtttataata gatgatttta taaaatacta
    30121 tattgtaata gcttttagct ttcaaataaa tattagcaaa ggatggaaat gacaatgata
    30181 aaactaaatg ataatttata aatatctcct tacgatatat aataattgta gggagatgtt
    30241 tctttttttg tatagtataa ttttttctag aaattctgtt tcaatatgta aaaagttggt
    30301 atatataaat agtataaact gtgttgctaa aatatggtag atattatata atataagtgg
    30361 atatgtatat acattatgag ttatataatt ttgaatttaa taaaatagaa acgttgattt
    30421 atgttctgta atgtgggcac cttggagcat attgagttag tggtgcaacc ggctatgaaa
    30481 ttgtatttat ttatagatac tattattttc atagcttttt ttatttaaaa aatggttata
    30541 tattagaaat aaatttggtt agtacgttta aataatttta cttggatttt taaccagata
    30601 tattataagc aagtattaat taaaaagttg agggggaata aaaatgaagt taaaaaagaa
    30661 taaaaaaggt ttcactttag tggaattatt ggtagtaatt gcaattatag gtatattagc
    30721 agtagtggca gttccagctt tatttagtaa tataaacaag gctaaggtag caagtgttga
    30781 gtctgattat agttcaatta agagtgcagc attatcttat tattcagata ctaataaaat
    30841 accagttaca ccagatggtc aaactggttt aaatgtttta gagacttata tggaatctct
    30901 tcctgataaa gctgatatag gtggagaata taaattgatt aaagttggta ataaattagt
    30961 attacagata ggtaaagatg gtgaaggagt taccttaaca gaagcgcaat cagcaaaatt
    31021 attgagtgat ataggtaaag ataaaatata tacaggtgtt acaggagata attttggaga
    31081 gcaattaaaa gatactacaa aaatagataa taaagctcta tatatagtac ttatagataa
    31141 tactgtgatg gattcaacaa aatagttaaa acaatatttt tataaaaata ttgtaaattt
    31201 aatgaaaaat ataatataaa agacaggaag aaaatcttcc tgttttttta tactattttc
    31261 tttaatgttc aattatgaat gatgatataa ttgagatagt aaaggagtga aacctgtggc
    31321 taaaaaagtt agaataggag acaaactggt cgaaaaagga tatataacag aagaccagct
    31381 taaatgggca ttatcagaac aaaaaaacag tggaaaaaga ctaggggaat ttctagtaca
    31441 agaagggctt atagatagta acttattaat aagtgtatta aaagaactct tagatataga
    31501 aagcatcttt ttagaaggaa cagaaataga tacattagct acaaagatgg ttccagaaaa
    31561 tatatgcaaa agatacactg tatttccatt taaaattgat ggaaataaaa tatgtctagc
    31621 catgtcagac ccacaagaca gagaagcagt tcaagatgta agacgtatgt ctggtaaaga
    31681 cgtagagata tttatatcaa gcacagaaga tataaacaaa gcaataggtc atgcttatgc
    31741 acattcagaa ataaataagg caatgactga gtacaacaaa aatagaactg gtggagtaag
    31801 agaaacagta atacttgaag aagatgtaaa tgctgctcca atagtacgat tagtaaataa
    31861 catactagaa aatgctgtta gaatggaagc aagtgatata catatagaac aaagtgaaaa
    31921 ttacatgcgt gttaggttta ggatagatgg aatgttaaga gaatatatga ggatgaactc
    31981 agcaccatat aaggcagtaa tcagccgtat caagataatg tcagatataa atatatctga
    32041 aaagagaata ccacaggatg gtagaatcta tttaaaagta gataataaac ccatagactt
    32101 tagggtttct acaatgccaa ctaacagaga tgaaaaaata gccatgaggg ttcttgataa
    32161 gagtaatttt atggttagta aagaagtact aggaatagat gagcatggct ctaagatata
    32221 tgatgaatta ataaacactc cttatggtct tatattggtt gttggcccta ctggaagtgg
    32281 aaaaactact acattgtact caatgctaaa tcaactaaat acagaaaata gaaacctatt
    32341 aacaatagaa gaccctatag agtatgaatt acctggtgta aatcaatctc aaataaatga
    32401 aaaggcaggg cttaccttcg ctagtggtct tagagcattc atgcgtcaag acccagatat
    32461 aataatggtt ggagagataa gagatactga aacagcagaa atagcaatta gagcttctct
    32521 tacagggcat ttagtcttaa gtacattaca tgcaaataca gcagttggag caatttcacg
    32581 tcttttagat atggatgtgg aaagttttct gataacttca tctgttttag gtgtaatatc
    32641 tcaaagactt acacgaaaaa tatgtgagca ttgtaaggtt tcatatgaag cagatattgg
    32701 agaaaaaaag gctcttggta ttgatgtaaa tgaatcagta actatttata gaggaaaagg
    32761 ttgtgaaagg tgtaataata ctggatataa aggacgtcta ggtatatttg aaatgcttga
    32821 aattactcca gaaataaaag agttaataga ttcatctgca aatcaaagag aaattttaaa
    32881 gatggctaga aaacaaggta tggttagctt aaaagaggat atagttaaaa aggttttaaa
    32941 tggaaagaca acagtagaag aaatgattag aataattttg atgactgatt aaaggatgat
    33001 attatgaaaa gttttaaata cacagtaata aaagaggacg gaaaaaagca aactgatgtg
    33061 attgaagcca acgattttaa tgaagctaga aatctcctta gaaagagaaa tcttagagtt
    33121 attgaaatca aagaaagtaa aaataaaaat ataagattat ctagtagaaa gagaaaaaaa
    33181 gacttaggag ctgaccagat aagccacttt tgtagacagt ttgctattat agtatcttct
    33241 ggtataaata gtatttctgg attagaaaca ttagctagaa ggtcaactaa cattacatta
    33301 agagaagaaa ttaatcgtat agtggcagaa ataaaagtag gttctactat tgcagattca
    33361 atgctttcat caaaatcaaa gtttccaaag ttacttggtg ctatggtagc tacaggtgaa
    33421 gctacaggta aattagatga agtattaaaa agtatggctt cattttatgc aagtgaacac
    33481 agggttaaac aaaagcttag aaatgctgca acatatccat taatagtttt gatttcttca
    33541 tttataatga tttttatatt tacagcattt atggtgccta agatgattaa ttcaattaca
    33601 acagtaggag caactcttcc acttataaca agaattgtta tggggtttgg tatgtttatg
    33661 aagaagtttt ggataatagt acttttagtt attatattat ttatctatca gtttaaaaag
    33721 tatctaaaaa cgccaattgg aagagctcat aaagatagag ttataaacaa gatacctgtt
    33781 ttaggaaaag gaataaactg tatggtagct acacggtttt caagagcatt gtatctattt
    33841 gtatcaacag gatatccatt ggttcaaggg cttgattaca ttatagatag tgtaaataac
    33901 accatggcag aaaagatttt agtatcagct aaagacggaa taacaagagg agaaggttta
    33961 gcagaaaatt tagagagata tacatatttt gactctgtat tggtacagat gatagctata
    34021 ggggagcaga caggggaatt agagaatata agtaaacaga tggccgaatt ctatgagtat
    34081 gaatccgaga tttatttaaa taggatggca tcaatgatag agcctgtttt aataattgca
    34141 gttggtatta tagttgcgat actggttgta tcaatattta tgccaatgct tagtatatat
    34201 gatgctatgt aggagggaaa tgacaaatga acaaggttta tgtatcatat tatggcgatt
    34261 ctatttccat agttgagggc agatataaac acaataagga taagttttat ataaaaaatc
    34321 ttaatgtgtt ttcaatagag gatattgcac aagatttttc taaggaaaag tatgctctat
    34381 tgagatacgc attagaaaat gttaagttaa aatcaagaca tgtagttttt tgtttaaata
    34441 caagagatgt aattttaaaa ccccaaaaac ttcctaatat ggataaaaag gatttagacg
    34501 gatttatgaa gctagagatg gacgaaatga tggctttgga gtcagatgaa tatgtatttt
    34561 cgtatgaagt atctagtgaa acacctgata attcagatgg cgatgaatca agcttaaatc
    34621 ttatcattgg agctatacaa aaggaagagg taaatgcaat tgtagagata atacatgaat
    34681 ttgatttaat attagataga atagatacat tatcaacagc ttacttaaga atgcttaaaa
    34741 atttagatta caatgatata atggtaacta atataagtga caatagcaca attataaata
    34801 tatacaagaa agatacttta tttattagtg ataatatacc tataaagata actgctaaag
    34861 atatggacat gcaattatta aatgtagtag aagaatcaaa aggtcttatg aattactatt
    34921 cgtcaagaaa ctttggtaag gttacagata ctatattaat attaggaaaa aggtattcca
    34981 ataaattaat ttatgaaact tttagctctg cattttcaag ctatgtttct caaggtctta
    35041 ctgagataat ggatatatct gatatgataa ctggagatat agatgaagat gatataaatt
    35101 caattgtaga gcttttagga agtatgatag aacctaaagg gaaaagagat tttgaaaata
    35161 taaacttttt accaatggat atacttagaa aacagctaaa agctaaacag atgaaaaaca
    35221 tactgaaaat tgctccagta gttattttga ttcttgctat accttattta tgtttaattt
    35281 tttcaaataa tttagcaaac aaggaacttg aaaatgttaa tgctgaaata agaaatattg
    35341 aatctgcata ttatcagatt ggaaatatag aaaaaatgat acagagtaaa agcgaagaaa
    35401 taaaggtata tgatatgtta attggaaaaa aggtaaagtg ggaaaatttg ttggatgata
    35461 tagataaaaa tactccaagt aaagttgagc taacaagttt atcaacgact tatcaaaatc
    35521 ttggagctaa taataaagct gataactcaa gcagtaataa gactacaaca gataaatcaa
    35581 gtaacaatga aacaaaatca aatacgaaaa gtgatagtaa tggagataaa acaaataatg
    35641 atttaaatga agataagact acaaatgtgt cagaagcatt aactggaaat agtgagacta
    35701 atagtaataa cacagataaa gatgataaaa ctccattgta tgataagata ccaaatgtta
    35761 taaacataga aggaaatgct aaagattcct cttatgtagg tcaattttta tataaattaa
    35821 agggattgta ttatttcaag gatgttaaat tacatagcat aaaaagtaat gcagaagatt
    35881 ctacctatac atttagtatg acattatacc tgaaagaagg tgtattaact aatgaataaa
    35941 gatatattta aaaaagatat aaaagatata tttaaaatgg aagttagtct aaaaggggtt
    36001 cttataaagg ttataatttt attatctatt ttatcatatg gttactttgg actttaccct
    36061 caatatcaga aatatattgt tacaaaaaat tctgtaaaac aagctactga aaaattaaat
    36121 atatataaag agaaaactgc acttatgcca aaatatgaaa aacaacttga agaagttgaa
    36181 agtgagttaa aagaaaaaag caaaaatctt ttatatgata tgcaagatgg tcttttttta
    36241 ataggtttag ataaaaaaat gaaggcttat aatattgact taactagtta tgacgttgga
    36301 aatgttagag aatacgaaca tttttatggt gtatcaacaa caataagtgt tagaggtgat
    36361 tatagaaatg tcaaaaaatt aatggcttat ttagaagaac aaaaaaatgt tactcaaata
    36421 ctagattata caatgacttc atatatagaa ccagaaaaag aagaggaaga aacaacaaac
    36481 aataaggatt taattaaaaa tgtgaacata aataaagata caaaaatata ctgggtggaa
    36541 ggcgaaaata agtggcattt atataaggat tgtccattga taaaagaggg agagattcta
    36601 aaaaatgaca aacttttaga taggtctact agttatctat gtaatacttg tgtacttaga
    36661 gctgcaacag aaaaagaagc aaaaaaagaa gtcgtagctc caaaggcaga aggtgtagta
    36721 gatgctacat ttaaatttat aatgtacact aaagagaata ctacaatgat acttgataca
    36781 gataatccat cttcatggag accgggaaaa tataatccat ttaagtctag tgcgaaataa
    36841 agctaagtaa gctttaaaga taggtgatga gtttgtacaa aaataatgaa aaaggtctta
    36901 ccttattaga agtaataata gctgttttta tactgacaat agttttaagc atttcttata
    36961 aggtgtttaa tggtataaca tcagcagtaa aaaagcaaca gattattaca gatgctcaag
    37021 taaatattaa cctaattaat aagtatctaa atagagattt ggaaaactgt aaagagctaa
    37081 ctaaaactgg ttcaggtagt aattatgaat ataatataga gacgccagat aatattgtaa
    37141 aatatgaagt ttccatagaa actaaaaaaa atacagaggt atattctgta acaagaatcg
    37201 aaaaaaatac aattgataca gaaaatgaag ttagagaaga gataatctat aatcaaccac
    37261 tagttcaaaa caataaagaa atgaaagaaa caccatttaa aatagaaaag caaactgata
    37321 aatctattta tactgtaagt atattctata atgaatcagt tcaagaaggt cataaaaata
    37381 gcaatttaaa taacaagact tatacatttg atgttatgtc gagaatagga taataagaat
    37441 ttattaaaaa taagtaggtt gataaaatat gagtaaaaag aatagtaaaa gaggattttc
    37501 cttaattgaa gtgttagtag ctatggctat tatgggaatt atactatttg cgttttttaa
    37561 tattatcaat actaataata aagcaaatat taaaaatgac acagatataa attccttaaa
    37621 ctatgtccaa agtgaaatag aaaatctaag agagaagata aaaagtggag aatttgattt
    37681 tgatagttta gataaaatgg aagatgggac tgtcgtatat gaaaaattaa tagataagtc
    37741 aaaaaaaatt gtgtatgata aagttcttag tgagggtaac gtgagcttat atgatactcc
    37801 atatgaaaag attacaacaa taaaagatga agatggtaat ttaatcgata aagaaaatat
    37861 tacaaataag ataaagacta ttgtagaaga taaaagtggt cagatatata aaatagctgt
    37921 aactgggaaa agcatgaatg actattcaag taaaaaagaa gttaagattg taactgaaat
    37981 atttaaagat aaataatttt acttgggggt gagattttga ggaagtggaa taaatttaaa
    38041 agtgaaagag gggcagcttt agttttagtt ctggttgtag tagcattact ttctattgtt
    38101 ggattgatgt tttcaaatca gatagccaat agaataaaat ctacaaagac tactaatgag
    38161 ggtatacaag caaagtattt agcagagaca tgtgtggaaa atagtattga taaagcatat
    38221 gaaaaattgt atgatgagct tgagaaaatg gataatgagt ttaaaagtga aaatcaacaa
    38281 aaatccatat ctaggtcaaa attaagaaat atatcagatg aagattttaa taatcaaaat
    38341 gaagaaaatc ttgaagtaga aagactagga tatatgaata atataaattt ctatttaaac
    38401 aaagcttcct cagatttgga aaaagctagt atagaattga aaaaattata tgatttagat
    38461 atgttggatt acagagacat tgaatatgta gatgaaaata ttatttcaca tagaaattct
    38521 attttagata tttgtaagaa ttatacttct ggagatatta gtaaaataaa tgaatatatt
    38581 ctggatgaag atataaattc aactacgtta gttgaagcaa agttagtaaa taatgatatt
    38641 cttttaaaaa tgtttttaga agaaaataaa attgaaaatg aacatttaaa ttcagcgttt
    38701 tcatacactt ataaggcatt agataaaatt tctttggcga tgcaaaatat gattgagtat
    38761 aggcatacat ttcatataga tgagcaaaaa gtagaagtat ctaatgggat tccaaattct
    38821 caacaatatt atgaattaat acaaaatcct ataataaata gtaaagagta tagctggaat
    38881 agtaaatggg atacattaga aaatctattg gaaactttac caaatcaaac tcaaggtttt
    38941 aattctttaa gagttcattt gagaaataat gtaagaaaat ttgaggagtt aagcgataat
    39001 atatctagtg gtaaaaaaaa tacagcaaaa aatttcttaa agtataaaga gttattgtat
    39061 gaaatatcag accaatgtaa tcaactaaaa agtatgtctt atgagaaaat acctgttaaa
    39121 tatgataaca tggccttgat aacaactttt gactatatac aaaatgagct tttagcagag
    39181 ataaaatgta gattaaaaga attaaaacct caagaagttg ataaaacaga agatattact
    39241 ataaaaatac ctttttataa ggctgactac gacatgacaa aagaaggatg gcctaaatta
    39301 aaagaaaatg gttcaggggc ggagttaagc ttaatggtaa ctggtgataa agatggaata
    39361 aaagaagtgg aagttacaga tagtaaaaaa aatattattg gtttaggtgt tgaagaaaat
    39421 tcaaattcaa agtataaagt tgaagctatt ataaatttta atctaaatat agatacaaat
    39481 atagtcggaa attatgatat aaaagataaa attttgatta atcatgacat aagttcatac
    39541 aaaaaagtaa actagatata ataagtaact tagtgtgaat aaataataaa ataattttat
    39601 tatttactac taaaaaaata agtaaactaa aaaaagatat ggagattaaa tattatgtat
    39661 atttatgatt tattagagca aggtataaga ctaaacgcat cagatataca cataacagtt
    39721 ggaacgaatc ctgtagctag agtaaaaggt ggatttgtaa aactaagtga acagatattg
    39781 acaccagaag taacaatgca aatggcaaaa gacatagcag gagaatctat gtttaaagta
    39841 ataaaagaac atggagaagc tgatttttca gcttctctaa aaacagggga aagatttagg
    39901 gtaaatgcat ataggcaaaa aggaaattac gcaatagcaa taaggactat aacagcagaa
    39961 attccaacat ttgaaaaatt aggtcttcca gaaaatataa aatcttttac tgaaaagcat
    40021 aaaggattag ttttagttac aggtccaaca ggaagtggta aaagtacaac acttgcaagt
    40081 atgataaaca taataaatga aaaacaacaa aaacatataa ttacacttga agacccgata
    40141 gaatatgtac atcatcataa acaaagttta gtaaatcaaa gagaagtagg aactgataca
    40201 gaaagttttc attcagccct tagagctatt ttgcgtcagg acccagatgt aatactaata
    40261 ggagagatga gagaccctga aactgtttca atagcattaa cagcagcaga aacaggtcac
    40321 ttagtatttt caacactaca tacagtaggg gcagctaaga caattgatag aatagtagac
    40381 atgtttcaac catcccaaca gcaacaaata aaaacacagt tatctacagt ctgtgaaggt
    40441 gtggtatcac agcagttact tcctacagca gatggaaaag gcagaatagc agcaattgaa
    40501 ttgatgtttg ctactccagc aataaaaaat ctgataaggg aaggcaaaac atatcaaatt
    40561 ccaaacatga tacaaactgg agttaaatca ggtatgaaaa ctatggacca agatttaatg
    40621 gaactttata aaaatggaaa gattacaaaa gatatggcac ttagtaggtg tactgaccaa
    40681 gagtttatga caagaatgat aggtggagta aactataatg gatattataa tcgttaatct
    40741 atatgtattt atagtgggta tagtgtttgg aagcttcttt aacgtctgca tttttaggat
    40801 tccagaaggt atatctattt ctagacctcc ctcccattgt atgagttgta atactagact
    40861 aaagccaata gacttaatac cagtgctaag ctatttaata agtggcagaa agtgtagata
    40921 ttgttatgag aaaatttcta gtagatatgc tatagtagaa ttaattacag gaattttatt
    40981 tttaatagta tttatgttat atggaatatc tacatcaact gtttactatt tggtacttgt
    41041 atctttactg attataatta catttataga tattgaccac tttataatac cagatgaaat
    41101 actgattttt ggagctattt ttgctataat ttttaacctt ttatttaaag aaattccagt
    41161 taaggattca attataggtt tttttatttg tggaggaagt gtctggctta tagttctttt
    41221 aattgaattt gtaataaaaa aagagtgtat gggtggagga gatattaaac tatttgcaat
    41281 gctaggtctt tacatgggtg ttaaaaatag tcttttgaca gctttactaa gcgtctatgt
    41341 gggagcagtc tatggaatct gtgtaataat tataagtaag acaaaaggaa aggaatataa
    41401 ttctattata ccatatggtc cttttatatc tgttggagca ttaataacaa ttttatgtgg
    41461 aaaacaatta ctcgaatttt atttttctat gattacatag ataatagtca agctaaatca
    41521 agttttaatt taaaaaaatg aattaaagta ttcaataagg acaatattat tattaaccct
    41581 attactaaag tgctacaaac tatggagtat ataataataa tttccttatt ttttgcacat
    41641 ttgataaata aatctctatg tgttttttgt gatagagaaa agaaaaactt atataatacg
    41701 ttttttattt acataataac tgtagtttta tctgtattaa tatatctaaa atttggaatt
    41761 agtgtagaat gtgtacaata tatggtttta attccatttc taataattat atccattgta
    41821 gattactaca caatatatgt atatgatata acaatagtta gtggtataat agttcaaggt
    41881 ataatttttt tggtcttttg caatatagtt caatttgatt atataaatca tatatttgga
    41941 ctagtgatag gctttattat accttatatt attgctaaat taacaaaagg attaggctat
    42001 ggagatgtag gtctctatag cttatgttgc tttgcattgg gaaataatta tgggttttat
    42061 gtgatagttt tatcatttat tttagcgttt ttgtttttta ttattttata cataactaaa
    42121 atatttaaag atatagatat aaaaaagcaa attcctttta caccttttat ttccttagca
    42181 acaattttaa taatgtttac agagtataat atatttaatt tttattatag tttgttatac
    42241 aaatttatat aacaggaggt aagaaatgta cgtagtagta gggttaggta atccaggaaa
    42301 gaagtatgag aagactagac ataacgttgg atttgatgtg atagatattc ttgctaaaga
    42361 atacaatatt agtgttacta aaataaaaca taaagcttta attggtgaag ggagagtagg
    42421 tactgaaaaa gtattattag ttaaaccaca aacttatatg aatctaagtg gagaaacatt
    42481 aatagatata tataaatatt acaaggttga tttaagtaat atagtagtag tatatgatga
    42541 tatagattta gaagttggaa agatacgtat aagaaaaaaa ggaagtgggg gaactcataa
    42601 tggcatgaaa tctataacta agtgcctagg ttcaaatgac tttccaagag taagagtagg
    42661 tgtatctaag ccagaagttg gtcaagactt agcagatttt gtactttcta gatttagaaa
    42721 agaagaatct gataacataa atgaagcatt agaaaaagca gcatatgcta ttgactcaat
    42781 aataagagaa aatatagata tgtctatgaa caaatacaat ggatagtgag agggagattt
    42841 aaatgaatga tgttttttta tatcctttgc aaaattcaaa agagtataaa gatataataa
    42901 attgtataaa aaatacaaaa ggttcattat tggttaatgg acttttacca gtacaaaaac
    42961 ctcatatatc ttattctata tttaatgatt tatcacgaca aatgattttt ataactagta
    43021 gtgatttaga agcaaagaaa gtatatgaag atttaagttt ttatattgaa gataaggttg
    43081 aatatttagg ttttcaagat atttattttt atcacttgga tgcaaaggac agaaatgaag
    43141 aagcaaagaa attaaaggta ttgttaaaac tagcaaataa agaaaaaata attttagtaa
    43201 cttctattga ggcagtttta agaaaatata taccaaaaca agttttgctc gatagtgtat
    43261 ctcattataa ggtgggagat aacttagact tagaaaggtt aactgaaaaa ttagtttcat
    43321 tgggatatga aagagtttca aaaatagagg ggtttggtca atttagtata agaggtggaa
    43381 ttattgatgt attttcactt gaatatacaa atccaattcg tatggaatta tttgatgatg
    43441 agatagattc catacgaact tttgacgtat attcacaaaa atcaattgat aaattgcaac
    43501 aattctctat aactccatct agggaattta tttatccaga aaaaactaca gatgcattag
    43561 ttaaactaaa aaaagaaaca actaaaaata ctgatgaagg tacatttcaa aatatagact
    43621 atatatcgag taagacttat tttgaaggtg tagaaaacta tatagattat atttatccag
    43681 aagaaaacaa aagtatattt acctatctag cagatgatgc aatagtattt attaacgata
    43741 taacaagact taaagaaaga tgcgaaaact atataaatga gtttagagaa aattataaac
    43801 taaatcttga aagaggtttg gctattaaaa accaaggtaa attgttatat cactatacag
    43861 atttagagta cttagtaaaa gacaagtctg tggtattaaa ctctctattg ccaaagtcta
    43921 taaataattt ttctataaaa tctataataa attttgaaag tagagaagtt cctaccttta
    43981 atggaaaggt agatttgctt gtagaagaat taaatcggct gaaatataat ggatacaaaa
    44041 taattttagc aacaaatacc ttagaaagag caaataaact aggaaaagat ttattggaca
    44101 aaggcttaga aacaaccatt tctaaagata gagatattga gattaaatct tcacaagtta
    44161 taattgtacc tgcacacata aacagtggtt ttcagtataa atctataaag tttgttgtta
    44221 taacagataa tgaaatgata ggtgtttata aaagagcatc taaaactacc aataaaaaag
    44281 ttaaaaaagg aaaaaaaata gaatcatttt tagatttaag tgttggagac tatgtagtgc
    44341 atgaaaatag tggtgtaggt agatacacag gaattgaaca aattacagtc aatgctatta
    44401 aaaaagatta tatgaaaata gtatatcaag gtggagataa tctatatgta cctatagacc
    44461 agatggacaa agtacaaaaa tatattggtg cagaagtaga aaaagttaag ttaaataagc
    44521 ttggaacaaa tgagtggaca aaagcaaaag caaaagttaa aaaagaaatt gaagatatga
    44581 caaaagattt gattgagctt tatgcgaaac gtgaaaaaat acaaggttat aagttttcaa
    44641 aagacacacc ttggcaggca gaatttgaat ctttgtttcc atatcaagaa acagaggacc
    44701 aattaaaggc gatagaagaa actaaaaaag atatggaatc aagtaaggta atggatagac
    44761 tagtatgtgg agacgttggt tatggaaaaa cagaagtagc tattagaagt atttttaaag
    44821 cttgtatgga ccaaaaacaa gttgctgtat tggttccaac aacaatattg gctcaacaac
    44881 attacaatac attcaaagag aggtttgaaa attatccttt gagagtagaa gttttaagta
    44941 gatttaaaac accaaaacaa caaaaacaga ttattgaaga tgctaaaaaa ggattagtag
    45001 atattttgat tggaacacat agaataatat ccaaagatat aaatttacca aacttaggtc
    45061 ttgtagtcat tgatgaagag caaagatttg gagtaaaaca taaagaagct ttaaagaaaa
    45121 ttaaatctac agtagatgta cttacacttt cagcaactcc tataccaaga acattacata
    45181 tgtctttaag tggaataaga gacatgagtg ttatagaaga acctccacaa gaaagacatc
    45241 ctgtaataac atatgttaca gagagtaagg aaagcgtaat tcaagatgag atagagagag
    45301 agttaagtag aggtggacag gtattttttg tatacaatag agttgaacat attgaagaga
    45361 tggcaagtat gattcaaaaa ctcgtaccag atgctagagt ggctgttgca catggaagaa
    45421 tgacttctaa atcattagaa aacataatcc taggattttt aaataaagat tatgatgtac
    45481 tagtctgtac tacaataatt gaaactggta tggatatatc caatgcaaat acaatgatta
    45541 tatatgatgc agataaaatg ggcttggcgc aactttacca attgagaggt agggttggaa
    45601 gaagttcaag acaaggatat gcatatcttt tatatgaaaa agataaaact ctaagtgaga
    45661 tagcagagaa gagattaaaa gctatcagag aatttacaga gtttggttct ggttttaaaa
    45721 tagcaatgag agaccttgaa ataagaggcg ctggaaatat tttaggttct caacaacatg
    45781 gtcatatggc agttataggt tatgatttgt atgttaaaat gcttaatgat gcaatcaaaa
    45841 aagttaaagg agaacctatt gtagaggaaa ttgatgttga aatagattta agtgtaaatg
    45901 catacattcc agataattat atcaaagatg agttaattaa aattgaaatg tataagaaaa
    45961 tagcttctat tgaaaataaa gaagacatgt tggatataca agaagaactt gaggatagat
    46021 tctctgatat acctaaacca gtccagacac ttcttacaat agcttatata aaatcgttat
    46081 gtaagatatt gaaaatagaa aagataagac aattaaaaga tgaaatatta ttagttccaa
    46141 taacaaagta tagaactaaa cagaaaatag gttataatat agttacagaa ttagaagaat
    46201 tattagaaaa aatgtgcaag gtaaaataac ttgtaaattt aagaagggat gttttaaatt
    46261 tgaaaaaggt aataacattg gtaatagcta tgattttggt agtatctgtg actgcttgca
    46321 attctagtaa aggagaaact gtggcaacag ttgaaggtac aaagatatca tcagatgaat
    46381 ttaaaaaaac tatagctctt tataaggatt ctatggagca aacttatgga aaagatatat
    46441 gggataagga agttgaaaag ggtgtaaaat acaaggacaa gtttaaagat ttaattcttg
    46501 accaacttat aactacagaa gtaatatatt ctcaagctaa aaaagataac ttacttccta
    46561 aaaaggagga tgttgaaaaa agctttaaag aattaaaaga tgctatgggg aaagatgaaa
    46621 aatataaaga acagcttaag aaattaggga tagatgatga gtttttaaga gaccaacaag
    46681 agaaagattt agcaatgcaa aactatcaaa gtaactttac taaaaagact aaaatatcag
    46741 atgaagaaat gaaaaaatac tatgatacac ataaagatga atttaaaaaa gatgaggtag
    46801 aagcttctca tattttattg aaaacagtag atgacaataa caagccactt tcagctaagg
    46861 aaaaagctga agctaagaaa aaagctgagg aggctcttaa agaagttaaa tcaggtgagg
    46921 actttgcaaa agtagcaaaa aaatattcac aagatgcttc agcaagtgat ggtggtaaat
    46981 taggattctt ttcaaggggg caaatggttg ctgaatttga agacgctgca ttttctatga
    47041 aaaagggtga agtatctgat ttagttgaaa ctcaatatgg ataccacata attaaagtaa
    47101 cagacagaat aaatgagcaa acatcatttg aagatgctaa agaaactata aaagaccaac
    47161 tattaaaaaa taaatatcaa gagcaaatag aaaaattaac taaagaagct aaagtagaaa
    47221 aagatgaaaa agtaataaat aaaatcacaa tctaagacct aaaaaagagt aatatgctta
    47281 aaaaagctat atctggataa gatatagctt tttttgtata tttttcttaa caatggtaaa
    47341 aataaactta aagtaattta tggagggatt aaaaaatatg aaagcaacag gtatagttag
    47401 aagaatagat gatcttggaa gggtagttat cccaaaagag ataagaaaga cattaagaat
    47461 tagagaagga gaccctttag agatattcac agcaaaagat ggagaagtca tacttaaaaa
    47521 atactctcca ataggtgaat taaatgaatt ttcgcaagaa tatgctgaaa cattagctga
    47581 agtactagga tatggagtgt tgataacaga cttagattct ataatagctg tgtctaagct
    47641 tcctaaaaaa gattataaag aaaaaagtat aagtgatgag ttagaagcaa taataagtga
    47701 aagaacagta aagtattcaa aagataaaaa cattttacca ttattcaaag aagaccaaaa
    47761 agaatatact agtcaagtta tcatgcctat aattagtgca tctggtgact gtataggttc
    47821 aattgtagtt gtttcaaagg acaaagatac aattgcagaa agtgaagaaa aatctttaaa
    47881 aattgctgct aactttttag gaaaacaagt tcaataattt tatcataaag accttttaca
    47941 tgaaggtctt ttgaagtttt tgtgtaaaat attgaacata gcattaaaaa tatgttatat
    48001 taaagtttgt tatataaaca gtgtggaggt tatgttatga acaataacaa aaataaagac
    48061 tcctttctaa aaggagccct tatacttggg ctagcaggtg taatcgtaaa aataatgggt
    48121 ggatttttta ggattcccct tggaaacatg attggttcag aagggatggg atactatcag
    48181 gctgcttatc ctgtgtatac tctattttta acattagcta ctgctggatt tccaacagct
    48241 gtggctaagt tggtttcgga aaaagttgcc attggaaatt ttaaaggtgc aaataagata
    48301 tttaaggtat cacatacagt tttatttatt actggtataa tatcattttg tatattattt
    48361 tttggagccg actacatagt aacaaacata atgaaaaatc caggtgcgtt atactctatg
    48421 aaagctattg ctccagcatt actatttgtt ccagcaatgt ctgcatatag aggttatttt
    48481 caagggagac aagatatgac taaaattgct gtgtctcaag ttgcagagca attttttaga
    48541 gttgtattag gtcttacttt ggcttacttg cttatgaaga gtctaggtca gacttttggt
    48601 gcagcgggag ctatatcagg agctacaata ggttcaatag catctatgct ttacttaatg
    48661 tttgcttata tgctagggag aaaagagcgt agagcagaga tagatgcaag ccaaagattt
    48721 aaagatgaga gagtgtctta tatttttaaa aagcttttaa ctgttgccat tcctataaca
    48781 ataggagcat cagtaatgcc acttgtaaat atgatagata atgttattgt aataagaaga
    48841 cttatggaag caggctttac atacaaagtg gcaaattcaa tgtttggtca attaacaggt
    48901 atggcaatgg ccacaataaa tttaccagca gtcataacaa ctgcaatgag tatgagctta
    48961 gttccagcta tttctaaagc atatgcatta ggaaataagt caaaagctag aaaagatact
    49021 aagagtgcag ttaaggtaac tttacttata gtattgcctt gtgcgtttgg tatggcttct
    49081 ttagcaatac caataatggg gcttttattt ccacatgaac catcttctgt aggaactatt
    49141 ttgtttactc ttactccatg tgtattattt ttaggattaa tacagacatt gactggtata
    49201 attcaaggaa tgggaaaacc aatagtacct gtaattgctt tatgtgttgg tatgctatgt
    49261 aaaattgtaa taagttatac tttaacaggt atcccagata ttaatgtact gggttcagcc
    49321 tttggtacag ttacagcgta ctttgtggca gccatgatta acttactata tgttaaaaaa
    49381 cacatgaatg taaacttttc taaaaaagaa tttataataa agccatttat aacagttatg
    49441 acaatgttta taatggttaa gttgagttat ggtgctttag ttggattctt aggaaacagc
    49501 atatccacaa taattgcaat atgtatcggt ggaattgtat atgtagtagt aatacttgga
    49561 ataggtggaa ttaaaaaaga agagattcta actatgccaa aaggtgataa attatataaa
    49621 ttgttaaaaa aagttaaact tataagataa tataggaata tatatgcaaa taagggaaat
    49681 actatacaaa tagtaaatct ccttatttgt atagtaatat aaaaataatg tgaaatttgc
    49741 taataggagt gatgttatgg gtaagataag tattgtaggt cttggaccag gagactattc
    49801 tcttataagc caaggtgtgt tagaatcttt aaaaaatagc cctaaagttt tttttagaac
    49861 agagaaacat ccaataattg ataaacttaa agaaactata caatacactt ctttagacta
    49921 cttttatgaa aaagaggaaa actttgacaa tgtttatttg aaaatatctg aatttatcat
    49981 agaagaatct gcaaagggtg atttagtata tgctgttcct ggtcatccaa gagtcgcaga
    50041 aaagactgta agtataatag agagattggc caatgaaaag cacatagcag tagaaatagt
    50101 gccttctatg agctttgtag acgctatgtt taactatttg gctatagacc catctgaggg
    50161 atttaaactg gtagatgctt ttgaaataga taattcatat atagacttgg atacaagtat
    50221 gataataact caggtatatg ataaatttat agcatctaat gtaaaattaa aacttatgga
    50281 atattatgac tataatcaag aagtatgtat agtaaatgga gcaggtgtca aagatttaga
    50341 aaataaaaag tttataaagc tttgtgaatt agacagaaat gaaaactttt ttgactattt
    50401 aactagctta tatataccca aaagttctaa aaagatgtat aatacagtac atgacttaga
    50461 aataatagtt aatacactta gaagcccatc tggatgtgaa tgggataaaa aacaaacaca
    50521 tcaatcatta aaaaattctg ttatagagga agcatatgaa ctttgtaatg caattgataa
    50581 taatgatata gatgaaatgg tagaagagtt aggagatgtt cttttacagg ttatattcca
    50641 ttgtcagata ggaaatgagg agggctactt tgatttaaaa gaagtagtaa atggaatatg
    50701 taaaaaatta attcatagac atcctcatat atttagtggt gcaaaactag atatgtctaa
    50761 gtttgaaaaa acttgggaag aattaaagaa agaagagaaa ggcgaaagta ctataacaga
    50821 tggccttaga aggataccaa aacatttacc agctcttatg aaggctagta aagttcaaca
    50881 taaagcagca cttgtcggct ttgattggga caatgtagaa gatgtattta aaaaaattga
    50941 agaagaatat caggaattac ttgacgaaca cagggtcggg aatataaaat acataaagga
    51001 agagctaggc gatttattat tttcgatagt aaatctagct agatttttag acatagactc
    51061 aggagaagcc ttgaactgta ctacagaaaa attcataaat agatttgagt ttatagaaaa
    51121 aagtgcaatg aacttgaata aaaaattaga agacatgaca cttgaagaga tggatgagtt
    51181 ttggaatcag gctaaaaaac aataaaattg tggaaggaaa tttgtcaaaa atctagaact
    51241 tttatcacaa agatagaagt ctatattata aacattttta ggaggtaaat atcgtgaata
    51301 aagctgaatt agtatcaaag atggcagaaa aaagtggatt aacaaagaag gaagcagaag
    51361 ctgcgttaaa cgcatttatg agttctgttc aagatgcact agtaaataat gaaaaagttc
    51421 aattagttgg atttggaaca tttgagacaa gagaaagagc tgctagacaa ggaagaaatc
    51481 caagagatcc agagcaagtt atagatatac cagcttctaa agcaccagtt ttcaaagctg
    51541 gaaaaggatt aaaggatata ataaatggat aattacaaat cttgtgaaaa agtgtggata
    51601 aatttgccac actttttcta tagagccaag gaaggtgata attatgagat tagataaatt
    51661 tttaaaagta tctaggatta taaaaagaag aactgttgct aaagatgcat gtgataaagg
    51721 gattgtcact ataaatggaa aagttgctaa atcttcttca gaagtgaata ttggagacac
    51781 attagaaata caatttggag agaagaagat gaagtttaag ataaatgaag taaaagagca
    51841 tgtattaaaa aatgatgcaa aagaaatgta cgaaatttta gaataaaatt aatagtcctc
    51901 tagtatatta atagtagtat tattgaaata tttactgggg ggatattatg gaacagaaca
    51961 taactttgaa agatagatca aagctggtta tttcgggagt tgagcacata tactctttta
    52021 atgacaaaag agtagagctc aaaacttctg taggcgagat ggtaattgaa ggcgaaaacc
    52081 tagatatgag caagttaagt atagacgaaa atataattag catagatgga actataaatt
    52141 ctatggtata tgcaaagcct aaaaagccac aagagagttt cttgaaaaag gtatttagat
    52201 agatgatacc ttttactcaa gatgtaagca ttttttatgc tactatttat ggtggaatac
    52261 ttataggggt gttgtttgat ttttatagag ggctaagagg aaattttaaa ttcatcaatt
    52321 attttgcaat aatatttgat gtattgtttt ggtttctagc tacagttata atattcgtaa
    52381 caataaacct tacagaattt tttgatttaa gatattatca ttttgtggct ttgtttattg
    52441 gttttatttt atactataac accataagta aaattgtttt atcaattatc aataaaataa
    52501 ttcgttttgt gagaaattct tttaaaaaag ttacacatta catagtcagt tttttaaaca
    52561 atttgtatta cgttataatc tattcactac acctactatt tgatattata ttttatatac
    52621 caaacatatt tatagcaaca agaaaaagta taaaaagaaa atcaaataaa aaatcaaaga
    52681 acaagaaaaa atcaaaacct aagaagaaaa aaaataaaac taaaaaaagg gtgtaggaaa
    52741 aatgaactta agaaaaaaat tttcaggaca gattattgtt atcagtttat ttttaggaat
    52801 atcaattttt agtatgatga caggatttgt atttgagtat acaaaagcta aggaatataa
    52861 aaaagaaata gcaagtctaa ataaacaatt aaaaaagact gaaatacaaa taaacgcctt
    52921 aaagaaagat gaaaaatcat atgaaggaga cttagaggat atagcaagaa aaagattaaa
    52981 tatggtaaaa ccaaatgaaa cagtttatgt agatataaat cgttgattaa aacttgctaa
    53041 taagtttaca gatgtattga ttttaaaatt taagatttaa taaaaaagtc attcagttaa
    53101 ggatggcttt ttgtttttat tgaggaggag attttataat gaaagtagga gctatagata
    53161 ttgggacaaa ctcaatgaga ttattgatag cagattatag agatggaaat ttcataaaca
    53221 gaaaaaaatt tgttaataca actagaattg gttatggtgt ggatagtaaa ggatatataa
    53281 aggaagatgc tattaaaaga aatataaaag cactaaaaga attttctaat aagtgtaatg
    53341 atgaaaagtg tgaaaaaatt tattgtatgg ggacatctgc tcttagagac agtaaaaatg
    53401 gagattattt tgttaaacta gctaaagaag aaactggaat agatgttgat atagttacag
    53461 gaatagaaga atcaaatttg ggatttaagg gcgttttaga aggaatacaa gatgatgata
    53521 ctattattgt tatagatatt ggaggaggtt caacagaatt tattgtaggt gactcagaag
    53581 gaattaaatt taataaaagt gaaaatgtag gtgctcttag gatgacagag agatttttga
    53641 agcatgatat tattgaagat gaagagcttg aagctatgag ttcatttata tatgatgaga
    53701 ttgacaaaac gttaaatcat ataaaataca gaggattaaa aactcttata ggtataggtg
    53761 gtacgattac atcattatca gcaatgaatc aagaattaga ggtttactct atggaaaaaa
    53821 tacataatag taaaatacac aaaaaagaaa tagaagaaat tctacaaaaa ttaataaaga
    53881 tgacattaag tgataaaaag aacttaaaag ggctacaaga aaaacgtgcg gatattataa
    53941 ctgctggtgt aaaaatattg aatataataa tggaaaaatt agaaatagaa gaaataaccg
    54001 taagtgaata cgataatttg gaaggcttaa tatgtcaaaa gtcaaaaaaa atgtattaat
    54061 attttttgat ttttaaaacc gattatgaca aaaaaaatta ctcctctttg ttacaattct
    54121 taatataaac aaaagaggag gtttttttat gcaaagaagt gtagcagttg tgaagaataa
    54181 gactgtaaaa ccaaatgtca taaaattaat aagcggatat attgatttta ctacggcggc
    54241 attcgctgtg atgggatttt tacttagtag gtctattata gtagactctg tagctccctt
    54301 aggaattgcc ttttttatat gtgcatcaaa gattgataag tacaaaatac ctgtattcat
    54361 atcaacatta ttaggaatat tgttatcatc taatgatttt ggaaatgtag caaaatatgc
    54421 tgtgtgcctt acaatattta tggctatgaa taaaaaatta aaattatcag attcaattgc
    54481 taaggttgca tttataggtg caataatact acttccaatc tccataggac aagctttctt
    54541 ttcaaatagg tatgtgtatg gtcttcttat tgcaggagta gaagcaataa taatgtttat
    54601 atctatatat gtgttttctt atggaatagg tttaatggcc aatgtcaaca ataggatgtc
    54661 tgtacggaca gaagaagcta tatcaataag tttactgcta acctttagca taatgggaat
    54721 aggagatata tccctgtttg gaatatcaat aaggaccatt ttatcgactg tattgatatt
    54781 actgacctcg atagtaggtg gagctaccat gggagcatct agtggtgtaa ttattggtgt
    54841 agcatccatt ttaaataata tgacatcagc tatttatatg ggaatatatt ctttttcagg
    54901 gttaataagt ggagcattta ataagataaa taaatatttc tgtatactag gatatattct
    54961 aagttggaca ataatatacc tatatacttc tggtattact tctaatatga tgcaattaag
    55021 agatatacta ttaggttgtt tgatagtatt ggtattgcca gagagattgt ttaataagat
    55081 tgaaaaatta ataaaaagta atgtagcctc aaatgaaatc gtatacgatt atattatgag
    55141 aagcaagaat ttgactaata gtcgattgaa cagtatttat aaaacttatg acgaccttgc
    55201 agatacgttt gataagataa gagaaaaaga caaagtatta gaccagagag atatagctaa
    55261 cgtaatagat atgattcata atgatgaatg taaaagttgt agtatgagaa gaatgtgctg
    55321 ggaatcaaga tttaatcata catacactat gatatataac attcttgaaa aaattgagga
    55381 gaaaggggag ttaagtctaa acgatatacc taaaaatttt agaaaggaat gtatgaaacc
    55441 agagtcaata gtgaaaatat caaatcacta ttacaaaatg tttgtattag attatgattg
    55501 gagtgtgaag ttctcagaaa gtagaaagtt aatagcaaat cagataagaa gtatttcaaa
    55561 atcaattaaa tcgttatctc aagatttaga aggtgacata atgctagata tagaaaagga
    55621 aaaaaatata tatgaacagc tagaaagata tgatataaca gttgataaag taagttactt
    55681 aacaaaaagt aacagtgagt ttgaaatatc aatagaaaag aaaacttgcc atgatggatg
    55741 tatgtgtgaa gataaaatcg taaatataat ttcagactta gtaggagaaa atatgtctgt
    55801 tagaaaaata gggtgtcact gtttaggagg taaatgtaag gcaacattcg ttaaatctca
    55861 aaaatataag gctgttacag aagtttcagc aatgtcaagg gatggacata ttctttgtgg
    55921 agacaattac acatacatgg agataaatga tggtaaatat atgatggcta taagtgatgg
    55981 tatgggaaaa ggtaaaaaag catatgaaga atcttctgcc actattgata tactggaaaa
    56041 aatgatagat gcaaaaatta aagacgaaat agttattgat actataaata atatgttgct
    56101 attaaagtct tcagaagaga tgttttcaac tttggatttg ggaattttag atttaaaacg
    56161 aggttgttta gaaactataa agatgggagc gtgttctact tatataaaaa gagaggatgg
    56221 agaagttgac ttaatatcat catcatcatt acctgtaggt atattatcag atgtaaaaat
    56281 tgatagaaaa aatgtaaaag ttaaggaagg agactatgta ataatggttt ctgatggaat
    56341 tgtagatgca ggaagaaata acaatttagg agataattgg ttaatatact tcttaaagaa
    56401 tatagagact acaaatccaa aagaaatttc aaatttaata ttggatagag cattagagtt
    56461 acaagcgtta caaatcgaag atgacatgac tgttttagtt acgaaaatat gtacaaaata
    56521 aggtttgcaa aattatgtgt ataagatgtg gataacttgt gtataatatg ttaatagact
    56581 tttgaaaaaa gtatagttat taacaaaaat aacatataaa tcaacaaaat ttgttgataa
    56641 gtatggcaga aatgttaata aaatgtaaaa aaagaaaaaa caaaagctat tatcaaaaga
    56701 gattcatgtt gaaataacta aatttctatg taaagaaaac ctgtgaatat gttgattatt
    56761 atgtggataa ctattaattc gatatttatt tatatagtat gaataattga ataatatatc
    56821 taaaaaaagc catcctatta ttaagtaaac ttattgaaag gatggctttt tatgaataga
    56881 aaattgtgca aagcaataat acctttattt atttctattt tttgcatgtt aataataagt
    56941 ataaaagctg accaaggata tcaaaagaat ggtgacagag caattactgt catgaataac
    57001 aatgatactg aagatgatgc tgaaaaaaaa caagaaatag actatgaaaa aacatgttgg
    57061 aacttgcaat ctttgtttaa aagtgatgaa cagtggaaaa aagaacttaa gagttttgat
    57121 aaagatacta aagagttgaa aaattacata ggcaaagtaa caaaatcgcc aacacatttg
    57181 tcttttgcct taaacattaa ggaaaaactt gatataagac taaataaact ttctgcctat
    57241 gtaaaattaa agcaagatac taataaaaat tcatataagt acttagatat gagtgatagc
    57301 atgagtaaat cctatgggaa ttatatgggg atttgctcag atttagaatt agaaatacta
    57361 aaattatcaa acaaagatta caagaaaatt atttcaaata aaaatataaa tagaaaatat
    57421 ggtgcatatt taagagatat aagaagaaat aaagtacatt atttggatga taaatcagaa
    57481 gatattctaa gcaaagtatc ttcaataagt agtcttccaa gccaagtata tgaattattt
    57541 agaaatatgg atagaaaaac taatttgaca ccagctcaat atgcaagtga attagagtca
    57601 tcagatagag aaaatagaaa aaaagcttat caagatgaac ttataactta caatgataat
    57661 ataaatacaa ttgcaggttt aattactggt caagttaaaa agaatatttt ttattctcag
    57721 gaaagaggtt atgagtcttc tttagaaatg tacttagaat ctgataatgt agatgaaaaa
    57781 gtatacaata atttgataag gactgtaaat ggtaatatgg acagtctaca caaatatata
    57841 gatttaagaa aaaaagtatt aaaattagat aaggttcatt catatgatat gtttgtgcct
    57901 atggttaagg ctgttgataa taatattaat tatgaaaagg cacaaagtat agtatattca
    57961 gcgttgtcac ctttaggaaa agaatacaat gatgtagtat ataaagcttt caatgaaaaa
    58021 tggatagatg tttattcaca tgaaaataaa gtaagtggtg gatactgctt atctgtttat
    58081 gaaaatcatc catatatact tttaaattat aataattcat taggctcagt ttctacttta
    58141 tcacatgagc ttggtcatgc tgtatacgaa tacttaagct ctaagaatca gaattatttt
    58201 aattcaaatc cttctatatt tactcacgaa gttgcatcta caacgaatga agcattactt
    58261 tacgagatgc ttataaaaga ggctaaaggc aatgatgaaa aagcttatta tataacacag
    58321 tacttagact tgataaaaga tactctttat acacaaacca tgtatgctga atttgaaaaa
    58381 actatacatg agttagtaga aaaaggaaaa agtgtaaacg cattagtatt aaatgatgtt
    58441 tggggacaac ttcttaaaaa atattatgga caaagctatg aattagacca attatctaag
    58501 gtcggatggg ctagaatccc acatttttat aatagtttct atgtctataa atatgctaca
    58561 ggttgtagtg caggagtaag ttttgcccaa gacatactaa aaaatggttc cgataactat
    58621 atgagctttc tcaaaaaggg aagttcagat tatccaataa acttgttaaa atcaagtggg
    58681 ataaatttaa ctaatacaaa gccaatagaa aatacaatca aaaaatttga cagtttagta
    58741 gcagaacttg aaaagctatt ataaacatta ttataatcca catggtaaat atatcgaatt
    58801 aatgataaaa tatactaaaa gtttaaagtc ctatcaagat aggacttttt atttattaat
    58861 tagtgggaga tgattctatg caagttaaag ttaaaaaggc tgtaatacca gcagcaggtt
    58921 taggaactag atttttgcca gctacaaagg cacaaccaaa agagatgttg ccaatagttg
    58981 ataagccaac tctacaatac ataatagaag aagctgtggc ctcagggata gaggaaatac
    59041 taataataac aggtagaaat aaaaagtcta ttgaagacca ctttgataaa tctgtagaat
    59101 tggaacttga tttagagaaa aaaggtaaaa aagaattgtt agagatagta caaaacatat
    59161 ctaacatgat taacatacat tatataagac aaaaagagcc aaaaggatta ggagatgcaa
    59221 tttattgtgc gagacatttt ataggtgatg agccatttgc agtaatgtta ggtgatgata
    59281 ttgtagataa tgatgtacct tgcctaaaac aactaacaga tgcatatgag gagtatagaa
    59341 ctacgatatt gggtgttcaa aaggtaaatc aagaagatac taataaatat ggcataatag
    59401 aagcaaaaaa tatagaaggt agagtttata aggtaaaaga catggtggaa aagccagagt
    59461 caggaaaagc tccttcaaat attgctattt taggtagata tataataact cctgaaatct
    59521 ttgatattct taaggattta ccaccaggta aaggtggaga ggtacaactt acagatgctt
    59581 taaaaatatt atctaagaaa gaagctatgt atgcatacaa ttttgaaggc aagagatatg
    59641 atgttggtga caagctagga tttttagaag ccactgttga ctttgctcta aagaaagaag
    59701 accttaagga agattttata aaatatttga aacatgtatg tagtgaattt gataaaaata
    59761 acaatgtgtt atacaataat acagatgaaa aaatagattt aattgaagtc aatgaaatca
    59821 gagaacttga aattcaaaaa taattaatga aaggtgatgt atggtggcaa aatcaagtca
    59881 gaaaagaatt aatagaataa tagctgttgg gattgtggga atccttgtat taactagtat
    59941 aacagctgtt ttgagtatga ttgcaactat gtaataaggt aaataacaga agttgatata
    60001 cataaatata aaaaccatat ataacttgat gtagttaaaa aattacaata ttttagttat
    60061 atatggtttt tataatttat aaatgtaaaa aaaatttttt ataatagttg catattttaa
    60121 aagaaaatat tattataaga tataaatata gtgataaatt tataaaaacg tgctaagatt
    60181 ggacttgaaa tacagtaata tagtaggtaa taatgtttta aattttattt attgaccata
    60241 atatataaat aatgatatta ttagacttaa ctgtttaaat aaaacttttc tttttcatgc
    60301 cttcaatttt caaaataagt atgtatataa atgaatatat agatgaggag gatttttttg
    60361 attttagatg ataaagtatt agaaggatta ggtagtaaga ccttagttga gctacgagag
    60421 atagctaaag aattaaaaat aaaatcaatt actacatata agaaaaatga gttaattgag
    60481 attataaatt ccaaaagtaa aaatgaggaa aaaataaacg aagagactaa agaaaaaaac
    60541 gaagatatta aagaagacat aaaaaataga aaagaaaata tagagcgtga aagtatagaa
    60601 aaagaagttg aatataagag ccctaccaaa tcatacaaca agaaagaaat agatacccaa
    60661 aatatttccc aaaaccaaga aaatagaaat caaagaaatg attataataa gacaacaagt
    60721 gtaaatgatt gtaataagcc atcaaatata tctaatagaa tggtcagaaa taataataaa
    60781 aactattata tgcctaaaca agttgatgaa tctaagatag tagatgaatt taacacatca
    60841 aaagaggatg aagtagtagg tgtacttgaa attctgccag atggatttgg ttttttaaga
    60901 ggttcaaatt atttgtctac tgaaggagat gtttatgttt caccatctca gataagaaga
    60961 tttaatatga aaacaggaga taagattaag ggaataacta gacatccaaa aagtggagag
    61021 aaatttagag cgcttttata tgttcaaaaa ataaatgatg aaaaccctga cacagctata
    61081 caaagaaatg cgtttgaaac attaacacca atatttccag aagaacgact tacactggaa
    61141 actaatagaa atgaaatagc tacaagaatt atagatttaa tttcacctat aggaaaagga
    61201 caaagagggt tgatagtagc acctccaaaa gccgggaaaa cagttctttt aaaaagcgtt
    61261 gcaaatagta tagctaaaaa tcatccaaat gttgaactta tagtactcct tatagatgaa
    61321 agaccagagg aagttactga catgaaagag tctattgaag gagatgttat atattctact
    61381 tttgaccaag tatcaagtca tcatgttaag gttgctgaga tggtattaaa tagagctcaa
    61441 agattagttg aacatggcaa agatgtagta attttactag atagtataac tagattggca
    61501 agagcatata accttacaat atctcctaca ggtagaactt tatctggagg tattgaccct
    61561 ggtgcactac atggacctaa aaagtttttt ggtgcagcaa ggaatattag acagggtggt
    61621 tctttgacaa tattaggaac agcgttagtt gaaactggtt ctaggatgga tgatgtaata
    61681 tttgaggaat ttaaaggtac aggaaatatg gaattacatt tagatagaaa attagcagaa
    61741 aaaagaatat tccctgcaat agatatttac aagtctggta caagaagaga tgacttattg
    61801 cttgatgatg aagaaaagac tgctctttgg agattaagaa gagaaatgag taataattca
    61861 gtaatggaaa ttacagataa agtcattgaa cttataaaga gaactaaaga caataaagag
    61921 tttgtaaaat caataaaaaa tttgtaaaat caataataca aatgtaaatg tagattatat
    61981 aaagtaatag aaaaatcact tgaatggcat aataagttgt gctataataa tgtagttgat
    62041 aatgtaaaaa gaaagttaat ataattaatt tttttaaata aagagaagag gtgactataa
    62101 tgcaaaagga aatacaacca aaatacaatc cagttgaagt gcgttgtgct tgtgggaaca
    62161 catttgttgc tggttcaact aaggacgaaa taaaagttga aatatgttca gagtgccatc
    62221 cattctatac aggaaaacaa aagaacatag aaaaaggtgg aagaatcgac aaattcaaga
    62281 aaagattcaa aatggactaa tttttataaa agtggagttg gaggaagcca actcctattt
    62341 tattacaaaa aaacattcat attctacttt aaattgtctt tttgtattat aataataaat
    62401 atgtaaaatt gagtattcta aatacaataa aatgaggtga gtggatggca aaacaagctg
    62461 taggtgggca agctgttatt gaaggtgtaa tgatgcagtc aaaagacaaa agagcggtag
    62521 ctgttagaaa aagtgacgga gaaatagcac taaaagaaga tagaataaaa agttgggtaa
    62581 gagataagaa tatagataag ataccttttg ttagaggttc ttttgttatg atagatacaa
    62641 tgattcaagg cataaaaagc ttaaactttt cttcagagtt ttttatggaa gaagctgaag
    62701 aggataaatt tgatttattt ataaagaaaa tttttaaaga taaggcaaat gatataatca
    62761 taatattttc attggttata gcaatgttac tttctgctgg attattcatt tttataccta
    62821 cattggtagg aggcgctttt tctaaagtaa tgcctaatga ttttatgctt aatttaatag
    62881 aaggaatcat aaggatagct atattatttg catatatagt attgatttca cgaagtaagg
    62941 atatagaaag agtctttcaa tatcatggag cagaacataa atctatatat tgctatgaaa
    63001 atgatttaga acttacagta gagaatgcta gaaaatttaa aagattacat cctagatgtg
    63061 gtacaaattt cttatttata gttatggctg tatcaataat attgtttgca ttttttggtt
    63121 ggccaaatcc aatccttaga atatttatga gaattatatg tgtgcctata gtagcaggtt
    63181 tatcttatga agtaattaga gttcttggga aatatgataa tggatttact aaaattattg
    63241 catatccagg aatgatgttg caatatttta caacaaaaga gcctgatgat gagcaattag
    63301 aggttgcttt ggaagcatta aaggcagtag tagattaaag aaaaggatat ttgttatgac
    63361 aataaaagat ataattatta aatattcaga tatattaaaa gatataagtg atactccaag
    63421 attggataca gagttattgt tacaaaaaac attgggtgtt gataggttat atattcactt
    63481 aaatctaaat aaagagctaa ctgaagagca aaaaacagaa tttataggtt ttgcagaaga
    63541 aagattaaat ggaagaccaa tagcatatat agttgaaaat agagagttta tgggattaga
    63601 tttttttgtt aaggaaggcg tattgattcc aagaccagat acagaaactt tagtagaaga
    63661 gataattgaa atatgtagag aaaaaaaaga tgtaagtata ctagatatag gcactggttc
    63721 tggagctatt acaataagtt tagcgaagta cattgaaaat tctaaaatca tgtcttttga
    63781 tatatcagag atagccttag aaatagctaa aaaaaatgct attacaaatg aagttgatga
    63841 aaaaataaaa tatattaatt ctgatttatt tacagcaata agtaatagta acataaaatt
    63901 cgatatcata gtttcaaatc caccatatat taaaaaacaa gatatagaaa ctctgcacac
    63961 acaggttaaa gattatgagc catataatgc tcttgaaggt ggagaagatg gattagactt
    64021 ttatagaaga ataacagaac aaggtaagaa atatttaaat aagtttggaa tattagcata
    64081 tgaagttggt cataatcaag ctgaagatgt aataaatatt atgaaaagta atggatataa
    64141 gaaaatatat acaaagaagg atattcaagg cattgataga gttgttatag gttacaatat
    64201 atgatataat caacaattga tatgttacat ttaatcgagg tgaaagtatg ttaaaaaaat
    64261 tagaggtatt ggaagataca tataaagact taagtgaaaa aataggtgat ccggatgtaa
    64321 taaatgatca aaaagtttgg caaaaatata taaaagaaca tgctgactta gaacctattg
    64381 ttatgaaata cagagagtat aaaagtgttt tagatagtat aaaagaatca aaagaaattt
    64441 tacaagaaga gtcagatgag gaattaagag aattagctaa aatggagtta gctgagatgg
    64501 aagaaaaagt agcacctctg gaggaagaaa taaaaatact attattgcct aaagacccta
    64561 atgatgataa gaacgttata gttgaaatta gaggtggagc tggaggagat gaagcagctc
    64621 tatttgcggg tgatttattt agaatgtatt caagatatgc tgaaagaaga agatggaaaa
    64681 tagaattatt gagtgcaagt gatactggag ttggtggata taaagaagta tcttttatga
    64741 taaaaggtaa aggtgcatat tcaagattaa agtatgaatc tggagttcat agagttcaaa
    64801 gaataccatc aactgaatct ggtggaagaa tacacacatc aacttctact gttgcagtat
    64861 taccagaagt tgaagatgta gaggttgaaa taaatccaaa tgatttaaga atagacgttt
    64921 tccgttcttc aggtaatgga gggcaaagtg tcaatactac tgactctgct gttagggtaa
    64981 cacatatacc tacaggtgaa gtagtatctt gtcaggatgg aaaatcacaa ttaaaaaaca
    65041 aagagcaagc tttaaaaata ttaaaagcta gactttatga taaagcattg gcagaacaac
    65101 acaaagatat agcagcagaa agaaaaagcc aagtcggaac aggagacagg tcagaaagaa
    65161 taagaacata taacttccct caaggtagaa taagtgacca tagaataaat ttaactttat
    65221 ataagctaga tgcattctta gatggagata tagatgaaat gatagatgct ttaattactg
    65281 ttgaccaaac agagaaaatg actgcaatat aagtaatgtt ttaaagaagc tgtttctttc
    65341 tagaaattat tttctatttt gaaacagcta ttttgcattt taggtcatgt tatcgtattc
    65401 tactgtatat aattgttata tacaagtata ggagggttat atgatactaa aagtaacaat
    65461 aattggactt ttggcaggtg ttatagggac aggattagga ggagtaatat cagctatttt
    65521 taaaagagag gttgataaat atctaagttt ttttatggga atatctggtg gaataatgtt
    65581 ggctgtagtg gtgtttgatt tgatgaaaga atctatggac aaaatgggga taatcaatac
    65641 agttatattt acgtttgttg gagctctcat aactatgtac ataaaaacaa aattagatat
    65701 gtctggaaat atggcatccg gatatcttat attcataagt atattactac ataatttacc
    65761 agagggacta gctattggtt cttcttttat gtcaactgaa agtttaggta ttacacttgc
    65821 tatagttata ggtttacata atataccaga agggttagct atggcattag gtttagtctg
    65881 taataaaatg aaactaagta aagttatttt atttactgta atagctggat tacctatggg
    65941 tctaggaagt tttttaggtg tttattttgg aggagtattt acttctctta taggagtatt
    66001 tcttgctaca gcaggaggta ctatgatgta tgttgtattg gaagaaattt ttccgcattc
    66061 aaaaagtgtg tattgtataa ttggtttctt gttaggtaca atgattgtaa actatatata
    66121 aaattaaaat gaaagcaggc atacaaaaga tgaaaactat aataagtaat attgatataa
    66181 ataatattga ttatgaggaa ataaaaatac aagcaaaact tcttagagaa ggtaaaacag
    66241 ttatttttcc aactgagaca gtttatgggc taggtgcaaa tgcattagat gaaaatgcag
    66301 taaagaagat ttatgaagcg aaaggaagac ctagtgacaa tccattaata gtccatatat
    66361 atgaaaaaga agaagtatat gatttagcca aagatataag tgataaagct aaacttgtta
    66421 tagaaaagct ttggccagga cctataacaa ttatattaaa taaaaaagat ataattccat
    66481 acaaaactag tggagggctt gatacagttg caataaggat gccttctaat attattgcca
    66541 gggcaataat aaaagaagct ggtataccta ttgcagctcc ttctgcaaat atctcaggtc
    66601 gaccatctcc aacaaaagca aaacatgtat atgaagagat gaatggaaga gtagatggaa
    66661 tagttttagg aggagacagt aattttggat tggagtcaac tgtattagat ttaacagaag
    66721 aaactcctat gatactgaga ccaggaagta taactaaaga agttttagag gaacttcttg
    66781 gagaagtaaa gcttgaccca tcattaagta aaaaagaaga taatcaaaaa gctaaagctc
    66841 ctggaatgaa atataaacat tattctccaa atgcagatgt atatatcata tctggaaaaa
    66901 gagaaaatgt agcaataaag attaatgata tgataaaatc taatagagaa aaaggtctaa
    66961 agacaggtgt aatgtgtact agtaaaaacc gagagtttta tgatggagaa gtaatcgatt
    67021 taggaaactc tcttgaagaa gttgcaagta atctgtttga tgctcttata gaaatggaca
    67081 aaaagagtgt tgatgtcata tattcagaag agttccctaa gactggagtt ggacaagcaa
    67141 ttatgaatag actacttaag tcagcaggat acaaagtgat aaaagcttaa aagggagttg
    67201 taaaatatga atatacttat tgtgtgtaca ggtaatactt gtagaagccc tatggctgag
    67261 gccatattaa agaaagcaat taaagaatcg ggaagaagtg ttgaagagta ttctatatca
    67321 tcagcaggta tatctacagc aaatggaatg agtgcttctg aaaattctat aaaagcacta
    67381 aaggaaatcg gtatagactt atcaaatcat agaagtaaag taattaccaa aaaattaatt
    67441 gatgaatcag atataatact aacaatgact aaatctcata aagaaatttt attacaagca
    67501 gttccaaaat gcaaagaaaa agtgtatact tttaaagggt ttgcaaataa aaatgaggaa
    67561 gatatatctg acccatttgg tggaaatttg gatagttata gaagcactat gagagaaata
    67621 gtgtattctg tcaacgaaat agtaaaaaaa atatgaaacg gaggataaat aaatgaagat
    67681 aggattaggc tgtgaccatg gaggatataa tttaaaaaaa gaaataataa gctatttaga
    67741 aggtaaaggt atagaatgtg tagattatgg aactaataat gcgacagatt ctgttgatta
    67801 tcctgtttat ggagaagtag ttgcaaattc ggttataaat aaagacgtag attatggaat
    67861 attatgttgt ggtacaggaa taggaatatc tttagctgct aataaggttc ctgggataag
    67921 atgtgcagtt gtatcagatg tattttctgc aaaaatgtca aaagcacata ataatgcaaa
    67981 tatgttatca ttaggagaaa gagttttagg aaaaggtcta gcacttgaga tagttgaagc
    68041 atggattaat acagattttg aaggtgatag acatgctaga agagtaaata tgattaaatc
    68101 aatagaagaa aaacataaca aataataaaa attacagttt tatcaggagg aattatcaaa
    68161 atgagcaaag tgatagagac aaaccatcca ttaatacaac ataaattaac tttaatgaga
    68221 gataaaaata ctggttctaa ggattttaga gagcttttaa ctgaaattgc aatgttaatg
    68281 gggtatgaaa taacaaaaga tataccatta aaagatgtag aaatagaaac tcctattcaa
    68341 aaaacatcat ctaaagtagt tgcaggtaaa aaattagcaa taattccaat tttaagagca
    68401 ggtcttggta tggtagatgg gttagtaagt ttaatgccag cagcaaaagt tggacatgta
    68461 ggactatata gagacccaga aacattaaaa ccagttgaat attactgtaa gcttcctcaa
    68521 gacataggag aaagagatat aatagtagtt gaccctatgc ttgcaacagg aggttctgct
    68581 gtagcagcta tagatttact taagtctaaa ggagctaaaa gtataaaatt agctaactta
    68641 gtagctgcac cagaaggtat agcagaagta caaaaatatc atgatgatgt tgatatatat
    68701 gttgcatcag ttgacgaaag attaaatgaa catggatata taattcctgg tcttggtgat
    68761 gctggagata gattgtttgg aactaaataa aaataaataa ctataaatat ttattaggga
    68821 ctttcttata tagtgtatat aattttatgt aagaaggttc ctttttatat attttctcaa
    68881 aataggaatt attgaattga taaatttctc gaattgagaa taaaaagttt ttaccaatta
    68941 aaaaagattg cttattttac agggttttat agaatagtag tctattttaa tgtggcatgt
    69001 tagttgctta cgattaagtg tatagatatt aagcgtatta gttaaattaa gtgttgaata
    69061 ttaataaata ttattgggag tggtaataca tgaagaattg tatagtagta agaggagctg
    69121 gagatttagc aacagcagtt attcataaat tacacaacag tggttttaaa gtagttgcgt
    69181 tagaaataga agaaccatta gctataagaa ggcaaatttc tttttgcgag gctgtttatg
    69241 aaaaaaaagt aactgttgaa gatgtggaat gcatattttg caatactgaa aaagaaatag
    69301 aagaagcttt atctcaacgt aaagtagcct tgattataga cccaaaagga gaaaaattat
    69361 ctaagttaaa tccaatcgtt ttggttgatg gaatacttgc taagaaaaat ttaggtacga
    69421 ctaaagatat ggcaccactt acaatagctt taggtcctgg gttttgtgca ggcgaagatg
    69481 tagatgttgt tatagagaca atgagaggtc ataatctagg aagaacaata actaatggta
    69541 gagcagaaaa aaatacagga gtaccaggag caataaaagg tttttctaaa gagagagtaa
    69601 tgcattcaca gcatgatggt ataatggaaa atgtatgtaa gataggtgat atagttgaaa
    69661 agggagacct actttgctat attaataatt atgaaaaaaa acatgaagtt agagcaacaa
    69721 tatctggagt tcttagaggt ttattaaaag atggagcaag ggtcaacaaa aatttaaaaa
    69781 tattagacat agaccctaga aaagaagaag ttaagaatgc ttttacaata tcagataaag
    69841 ctagatgcat agctggaggg gttttagaag ccatactaaa caatgggatt tatccccaaa
    69901 attaatttat actagataaa gctattaata ttttattaag atgatataat aaatttaata
    69961 gattaaagag agcatatgac gggtatttaa aatttaaatg gtaaaaatgc tcttttttta
    70021 ttggacattc aattaacatg tgtatataat ataaaaataa gtatttattt aggttgaaat
    70081 ctaataaata cataatacca gtacataagt aaagaggaga aaagtaaatt atgagaccat
    70141 catgggacga atacttcatg gaaattgcag aagtagtgaa aaagcgttca acttgtatta
    70201 gaagacaagt aggagctgtt attgtaagag ataaacaaat attaacaact ggatataatg
    70261 gttctccaag aaatcttgaa cactgtgaaa atattggatg taagaggcaa gagttaaata
    70321 ttccctcagg agaaagacat gaattatgca gagcattaca tgctgaacaa aatgcgataa
    70381 tacaagctgc tcataatggc attagtgtag atggggcaac tttgtatgtt actacaagac
    70441 cttgtgtttt gtgtgcaaaa atgtgcataa atgcaggtat attaaaaatt gtctatgaag
    70501 gtgattatcc agatgatatg tctacagaat tattaaaaga agcaggaata gaactcataa
    70561 aattttagta gacaaaaatt atatgacaat aagtgccaaa ttgtgacact ggaatacaat
    70621 ggtattaaat gattaaaaga aagtatttga aaaatcatca gattagtagt aagatatatt
    70681 ttataattaa ataaatttat gagatagatt aattacatgt tatttatttt gtaataatga
    70741 gggatactat ttatgagtaa aagaaataca catacagaaa ttgcaaagtt gtttagttta
    70801 ataagtcaaa taggattgat gattgtaata tcaatattag gatgtacttt cttaggaaaa
    70861 tttttggata gtaaattgaa tactactcca gttctgacaa taatattttt attgttgggt
    70921 gttggaggtg cttttagtgg tgtttataaa actctaatag tatatacacg aaggaagtga
    70981 tatattggat ataaaattac ttaaccaggt aaggtcagta acaaaaggta tagtaatatt
    71041 tgatgtagtt ttaattgtgt taatgttgat aacatcaaca ttaagtaaac cagtattatt
    71101 aggacttata tttggctcta taatagctat gttaaatttt agattattag cgatatcact
    71161 ggaaaagtca gtgacatttc ctgctgggaa ggcacaagct tatgcatcag cacaatatgc
    71221 tattagatta tttataatgg cagttgttct atttgcatca gctacggtac cacatttaaa
    71281 tataatcgga acagcattgg gattattgag cacaaagttt gtcatattat caaaaaactt
    71341 tataaataaa ttaaagagga aggaggcata agtatgaatc aagcgcatta ttatctcata
    71401 ggaaacttta agattagtga aacaatacta gtaagttggg gaataatggc ctttttagcg
    71461 atagcatctt attttttgac tagaaactta aaaaaagttc caactagtaa agttcaaata
    71521 ttcttagaat ttgcagtagg tggtcttgct aaaatggtta cagatgttat ggggaaaaat
    71581 acagtaaaaa gaatgccata tatgatacca tacataggaa gcttgttttt atttttcgca
    71641 tgttcaaatt taagcccatt acttggatta aggtcaccaa caaccgactt agacacaact
    71701 gctgcatggg caatgattac attttttatg atttattttg caggagttaa atttaagggg
    71761 ttatcatact ttaaagaatt ggctcaacca cttgccataa tgttaccaat caatattata
    71821 ggtgagttag ctagaccaat atcattaagt ttccgtccat ttgggaatat tttaggtgga
    71881 acaataataa tgggtctgtt ttatcaactt ttaggtttta tatctagttt gataccagga
    71941 ttatcaatac caattggaca gatattaata ccagtcccat tacacttgta ctttgactta
    72001 tttgcaggat tgttacaagc atatatattc ataatgttaa caatggtatt tatatcaagt
    72061 gcaggagagt tagattaaga aatcaactta tattaagata aaaataaaat aatttaaaaa
    72121 attaaagttt taaggaggaa ataattatgg aaacagcaat agtagcggca gcatcagcaa
    72181 taggagcagg gatagcagtt gcaacaggta taggtgcagg tataggtcaa ggtatagcag
    72241 cagctaaagc tgctgaagca gtaggaaacc aaccagaagc taaaggtgat ataacatcaa
    72301 ctttattact aggagttgct atagcagagt cttcagcaat atatggtttg gttatatcaa
    72361 taatacttct attcgtaaat ccattcttta aatacttagg aatgtagatt tagtccttaa
    72421 atattttgag ataggtttat aggtaaatca tctcaaactt ataatcatga gatgaacttt
    72481 atcatctcgt gattataagt agttatgatt tatggaaagg aggataatta tggagaaagc
    72541 actagtaggt ataacgtggg aatttgtatt ccaaattgtt aatactttta taatattttt
    72601 acttctgaga aagcttttat tcaagccagt attaaatata attgaatcaa gagaaaatga
    72661 tattaaaaat gatttggcag aaggcgaaaa agcaaaaaat gaaggtttag ctctgaaaaa
    72721 agaatatgag tcaaaaataa attttgccaa agatgaaggt caagaaatca taaaacaagc
    72781 aactataaga gcagagcaga aatcagatga tatagtaaat actgcaaaga aagatgctct
    72841 tgatataaaa gaaaaagcaa acaaagatat agaacaagaa aaacaaaagg ttataaatga
    72901 aatcaaaaat gatatatcta acatagcact acttgcagca tctaaagtta ttgaaaaaga
    72961 tttagataaa tctaaacatg aagaattgat agaaaacttt ataaaagagg taggggaagc
    73021 taaatgataa atgtaatagc aaatagatat gccgaagccc tatttcaatt aggagaagaa
    73081 gaaaattcaa ctgatgtttt attcaaagaa ttagaaaaag tagtagatat gatgactaaa
    73141 gttagtaaag atttctacaa agttttaaaa tcaccattag tttcaaaatc agaaaagaaa
    73201 aacttggtag aaattatttt tagtaaagaa gtaagttcta acataaaaaa ctttttaaaa
    73261 gttttagtag ataaagacag aatatcatat ttagaagata tcgaattagc ttataaagaa
    73321 ttattaaata agaaaaataa tgtaattgct ggtgtagcaa taagtgctat tccaatgagt
    73381 gaaacagata ttaaagaact tgaagtcaaa ctttcaaata aatacaacaa aaatgttact
    73441 atagaaaatg ttgttgacaa gactatacta gggggagttc tagttagaat tggaaatgaa
    73501 caaatagatg gcactgtgaa aactcgtctt gataagatga aagaaaagct atctgaagta
    73561 atatcttaag aatggcggtg aacccatgaa cttaaaacct gaagaaataa gttctataat
    73621 taaacagcaa ataaaaaatt atgagaataa agttgagttg acagatacag gtagtgtttt
    73681 aacagttggg gatggtatag cgagtgtata tggattagaa aaagctatgt ctggtgagtt
    73741 gctagagttc ccaggtgaaa tatatggtat ggcacttaac ttggaagaag aagtagttgg
    73801 agctgttatc cttggagatg acagtgaaat aaaagaaggc gatatagtta agagaactgg
    73861 aagaatagtt gaagttccag ttggagaagc tttaataggt agagttgtaa actctttagg
    73921 tcagccaata gatggaaaag gacctatagc gtacacaaaa actagaccag ttgaatctga
    73981 ggcacctggt ataatagata gaaggtctgt ttatgaacca ttacaaacag gaatcaaatc
    74041 aatagattca atgataccaa ttggtagagg tcaaagagaa ttaataatag gtgacagaca
    74101 aactggtaaa acatctatag ttattgacac tatactaaat caaaaaggaa aagatgttat
    74161 ctgtatatat gtagcaattg ggcaaaaacg ttctactata gctcaattag ttagttcttt
    74221 agaaaaaggt ggggctctag attatacaat agtagtttca gcaacagcat ctgaatcagc
    74281 accacttcaa tatatagcac catatgctgg agcagctatg ggtgaagaat ttatgtacaa
    74341 tggaaaacat gtacttatag tatatgatga tttaagtaaa caagccgttg catatagaga
    74401 aatgtcattg ttacttagaa gaccaccagg acgtgaggca tatcctggag atgtattcta
    74461 tttacattca agattacttg aaagagcagc taagttatct gatgaattgg gtggaggttc
    74521 aatgactgcc ttgcctataa tagagactca agctggtgac gtttctgcat atataccaac
    74581 aaatgttata tctataacag atggtcaaat atacttacaa ccagagttat tctattcagg
    74641 tgtaagacca gcagttgacc ctggtatatc agtatcaagg gttggtggtt ctgcgcaaat
    74701 taaagcaatg aaaaaagttg caggaacatt aaaacttgca tattcacaat atagagaact
    74761 tgcagcattc tcacaatttg gttctgactt agatgaagat actaaaaaga gacttgctca
    74821 aggtgaaaga atcgttgaaa tattaaaaca aggtgagcat caaccaataa aagttgaaaa
    74881 tcaagttatg ataatatatg ctgttataaa taatcatcta gaggatattc cgatagataa
    74941 tatagctaga tttgaatcag aattgtatgc atttgtagat aataattatc cagaaatatc
    75001 aagaaaaata ttaggtggag aagacttcac tcatgattta acagatgcaa taaatgaatt
    75061 taaagaaaag tttgttgttg aagtataatc ctttttaaaa aaggaggtaa attaattggc
    75121 aggagctgga atcaaagcaa tcaaaacacg tatgggaact gttgaaagta caaagcaaat
    75181 aactaaagcg atggaactag ttgcatcttc taagcttaga aaagcaaaag aaaaagctga
    75241 agattcaaga gaatatttta atactttata caatacagta aaagatattg cccaaacaac
    75301 acgtggaatt agaaatgaat ttttaaaaga aagagaagta aaaaataagt gctatgtagt
    75361 agtagctgga gacagaggac ttgcaggtgg gtacaattcc aatgtattaa aggagtctgt
    75421 ctctcatatg aaaggcaaaa aggaaacagt tattactgtt ggaaagaaag cgcatgaatt
    75481 tttttctaaa agaaactaca atatagtgaa agatataaat agtgttgaag cttgtggata
    75541 tgaagatgcg acagaaattt cacaaactat tattgacctg tataaaaaag gtgaagtaga
    75601 tgaagtttat attgcttata caaaatttaa atctgcttta tcacaagaag ttgaaatttt
    75661 aaaattgctt ccattagtct ttgaaaaaga tacagacgaa caaggaatgg aagtaaaaga
    75721 agagaaacaa aatagatcaa aagtacaata tataccatca gcagaagcag ttttaagtta
    75781 tattgtacct cagtttatat caggtacagt atatggtgga attttggaat cttttgcatc
    75841 agagcaaggt gctagaagaa ctgcgatgga atcagccaca gacaatgcga atgaaatgct
    75901 atctaattta gagttaagtt ataacagagc aagacagtca gctgtaactc aagaaataac
    75961 agagatagtt ggtggagttg aagctctaaa atagggaggt taggaaatgg caaatgtagg
    76021 taaagtagta cagattgtag gagctgtact agatgttaag tttgatagtg aacaaagcct
    76081 acctaactta ttaaatgcgt tagtaataaa gttaggagat aaagaaatag ttgcagaagt
    76141 tgctcaacat ataggtgatg atactgtaag atgtatagct atgagtgcaa cagatggact
    76201 tgtaagaggt atggaagttg tagatacagg aggaccaata agtgttcctg ttggagatga
    76261 gactttaggt agaatattca atgttcttgg aaaaccagtt gatgggaaac cagctccaaa
    76321 atctgcacct aaattaccta tacatagacc agcaccggcg tatgatgaac tagaaactac
    76381 agcagagata ctagaaactg gtattaaggt agttgactta ctagctccat atctaaaagg
    76441 tggaaaaata ggtctatttg gaggagcagg agtaggaaag acagttttaa tacaagagct
    76501 tataaacaat attgctaagc aacatggtgg tatttctgta ttttcaggag taggagaaag
    76561 aacaagagaa ggtaacgacc tttatggcga aatgagtgag tctggagtta taaataaaac
    76621 agctctagta tttggccaaa tgaatgaacc acctggagcg agaatgagag ttgctttaac
    76681 tggacttaca atggcagaac attttagaga tgagcaagga caagacgttt tacttttcgt
    76741 tgataatata ttccgtttca cacaagctgg ttcagaagtt tcagcacttc taggacgtat
    76801 gccatcagct gttggttatc aaccaacatt agctactgaa atgggtgcgc ttcaagagag
    76861 aataacatca actaagaaag gttcaataac atctgttcaa gcagtatatg taccagcaga
    76921 tgacttaact gacccagcac cagctacaac attctctcac ttagatgcaa aaacagtttt
    76981 atcaagacaa atatcatcac tgggtatata tccagcagtt gaccctcttg aatctacatc
    77041 aaggattctt gaccctagta tagtaggaaa agaacattat gaagtagcta gaggtgtaca
    77101 atcaatactt caaagatata aagaacttca agatataatt gctatacttg gtatggatga
    77161 actatctgat gaagataagt taatagttgc acgtgctaga aagatacaaa gattcttatc
    77221 gcaatcattt acagttgctg agcaatttac aggaaatcca ggtcaatatg ttcctgttaa
    77281 agagactgta agaggattta aagaaattct tgaagggaaa catgatgact taccagagtc
    77341 agcattctta tttgtaggga caatagaaga cgctgttaaa aaagcgaagg gaagtatgta
    77401 gtcatggcca gtgaattttc attgaaagtt gtaactccaa ataatacttt ttatgatgga
    77461 gacgttgaaa tgatagtagt tagaactaca aaaggagaca aaggggtatt gaaaggtcat
    77521 ataccatttg tttcaggtat acctgtagga actttaaaga taaaaaaaga gggaagtttt
    77581 aaagaggcga aaatagcaga aggatttgtt aatgtaactc aagataaaac tgtcatattg
    77641 acagaatctg ctgagtggat ttagtaattt aatttaaaat ataaaaagtc attttgacca
    77701 aaaaactttt gggtaaaatg gcttttttat attttactgt taatagaata acgataaaat
    77761 tgtaatcttt tagtgataat aaattcacaa aatgtttaaa tttactgcaa aattatgttg
    77821 taattaatat aataaagaag atttagatag cttttatgta agtgaaaaag tcttcatata
    77881 agaaatacat ataaaatttt tataaaattt aaaaaatttg cttagttgtg tagtataata
    77941 aataatataa tagatttgta tgtaatattc attacaaata attttaaaag ttacagtttt
    78001 attataatga ttattaaatt aagataagta acataaacta aataataata tgtattatcg
    78061 atagtaaatt cattattaat tattgaaaga agtattgtaa attttctgga ggaacaatga
    78121 atatatttga tattggcgta gacataattg aaattgacag aataagaaaa gctatagaaa
    78181 agaataatag gtttttggag aagatattta cagatagaga aatagagtat tttaattcta
    78241 agaattttaa agcagaaagc atagcaggta atttcgcagc taaggaagct ataagtaaat
    78301 ctataggtac aggaattaga gtgtttaact ttaaagatat agaagttctc agaaatgaaa
    78361 tgggaaaacc aatagtaaaa acttataata atctagcaaa aatgtgcata gattataatg
    78421 ttttagaaat aaaagtttca atatcacatt caaaagatta tgctatagca aatgcgataa
    78481 ccataattaa ggactaagga gtgattacat tatgatggat aaaacagcaa aagaaataat
    78541 gacaacagat gtaatagtag caaaacaaga tgatagtata gcagatgtag ctaatatgct
    78601 aattgcagaa aaaataggag gtctgccagt tgtagattca gagaatagag ttgttggaat
    78661 aatttctgaa acagatattc ttaaaaaaga aaaatatata gaagcacctc tctatataaa
    78721 tctattacaa ggattgattt tcttagatga cttaaaaaaa gtagaaaaag atataaaaca
    78781 agtagctgct tataaggttg gagaacttat gtctaaagat ataataaagg ttcatgaaga
    78841 tgataagttt gatgatgtag ctaatgtaat gattaaaaaa tcaataaaca gagttcctgt
    78901 agtagatgat gacaataaac ttaaaggtat aatttgtaga tatgatataa taaaagcttt
    78961 atacaatgaa taaactgaag taggaggtgt gatgtgagaa aaaagtggac catagtatgt
    79021 attatgttct tggcactggt tataattgta ataggatgcc aaaaacgaca gtccacaaaa
    79081 gaagaagtat acaaagattt tcaaaagcag atttctgata tgaattatta cagttgtaaa
    79141 gctgaagtag aagtagttgg aaataaaagc ccacataatt atgttttaat tcatacttat
    79201 aaaaaaactg ataactataa attagaggta atttccccaa agcatttaaa agggaaaagt
    79261 atagaatatc aaggtgataa aatattagtt aaaaatccaa agattagtga tatagtcgag
    79321 ttgcctaata caggaaaaaa taatcaatat ctttttatag gagactttat aaaaaactat
    79381 cttcaaaacg aggagatgaa agtaaagtta tccaaaggac atttggtttt agagactttt
    79441 attcctgggg ataataaata ttttaacaag caagtattat atgtaaatgc tgacacaaaa
    79501 aatcctgaaa aaatggaagt gttagataaa gagggagtgc caagatttac agtaaaatac
    79561 aaagattttg aatacagaaa ctaaggggga ctaaagacat gcaaaaaata acagtgccta
    79621 catgggcaga gataaatcta gataacttaa gatttaactt aaataatatt aaaaatttat
    79681 tagaagaaga tattaaggtt tgtggagtaa taaaagctga tgcatatgga catggtgcag
    79741 tagaagttgc aaaattgcta gaaaaagaaa aagtagatta cctagcagta gcaagaactg
    79801 ctgaaggaat tgaacttaga caaaatggca taacacttcc tattttgaac ttgggatata
    79861 ctccagacga agctttcgaa gattctataa aaaataaaat aactatgaca gtttattctt
    79921 tagaaacagc acaaaagata aatgaaattg caaaatcttt aggagaaaaa gcctgtgttc
    79981 atgttaagat agactcaggg atgactagaa taggtttcca acctaatgag gagtcagtac
    80041 aggaaataat agaattaaat aaattagaat atatagattt agaaggtatg tttactcatt
    80101 ttgctacagc tgatgaagta agtaaagagt acacttataa acaagctaat aattataaat
    80161 ttatgtctga taaattagat gaggctggtg taaaaatagc tataaaacat gtatcaaaca
    80221 gtgcagctat tatggattgc cctgatttaa gattaaatat ggtaagagca ggaataatat
    80281 tgtatggtca ttatccatct gatgatgtat ttaaagatag attagaatta agaccagcta
    80341 tgaaattaaa atcaaaaatc ggacatataa aacaagttga accaggtgta ggaataagtt
    80401 atggactaaa atacacaact actggtaaag aaacaatagt tacaattcca ataggatacg
    80461 cagatggatt tactagaatc caaaaaaatc caaaggttct tattaaggga gaagtgtttg
    80521 atgtagttgg tagaatatgt atggatcaaa taatggttag aattgacaaa gatatagaca
    80581 taaaagttgg agatgaggtt atactatttg gagaaggcga agttacagct gagcgtatag
    80641 ctaaagactt aggaactata aactatgaag tgttatgtat gatatcaaga agagttgacc
    80701 gtgtttatat ggaaaataat gagcttgtac aaataaacag ttatttgcta aaataaaacc
    80761 atgatattat aatctgggag gcgattttgt gtacgataag agtaaagaaa aaatagaagt
    80821 tactttacca gactctctga tttctgaagt tgatagtata gttcagatgg agaacagtaa
    80881 cagagaagat tttgcaaaag ctgcttttca gttctatata actcagaaaa agaaaattca
    80941 tattaaagaa tctatgaaaa cgggttatag agaaatgggt caaattaatc tgtcactagc
    81001 tgaattaggt atgacagttg aagaagtatc ttctgatgat aaaaatgaag ggaagattgc
    81061 ccaaggtgag tactaattgt gaaataaagc gtggtgattt atactacgct gatttaagtc
    81121 cagttgttgg ttcagagcag ggaggcgtta gacctgttct gataatacaa aatgatgtag
    81181 gtaataaata tagtcctaca gttatagtat cagctataac atctcagatt aataaagcca
    81241 aattacctac tcatgtagag ataagttcca atgaatatgg gcttaataaa gactcagtaa
    81301 ttcttttaga gcaaattaga actattgata aaaagagact gagggaaaaa attggttgtc
    81361 tagatgaaaa tatgatgata aaagtagatg atggacttca aataagtttg ggattgttta
    81421 ctttttaaag agggatttgt cttttattag acaatccctt ttttctttta aatatctctt
    81481 agtgtttaat tttaatgtac tatgttaata aaattaaaaa taacgcaaaa aaatagcata
    81541 aaagtgatat aataatatgg aattgatata aaattacgaa ctcaagggag gtaattgcat
    81601 gagagaccat aaaggattaa atgagattgc gcgtatcata agaagagata tagtttcaat
    81661 gatacataga gccaaatctg gacatccagg agggtcatta tcagtagttg aaatacttac
    81721 tgcgttatac tttgatgaaa tgaatgtgga ttcttcaaat cctaaaatgg aggatagaga
    81781 tagatttgta ttatcaaaag gacatgctgc accagcatta tatgctacat tggctgaaaa
    81841 aggctatttt gataaggaag aactgaatgg attaaggaag ataggaagga tgttacaagg
    81901 tcatccagac atgaaaggca cgccaggtgt ggaaatatct actggttctt taggtcaagg
    81961 cttttcagtt gcatgtggta tggctatggc atcaaagttg gataatgctc catggaatgt
    82021 gtacactttg cttggtgatg gtgaggttca agaaggaatt gtttgggaag ctgctatgag
    82081 tgctgcacat tataaacttg ataatttaat tgctttttta gataacaatg gacttcaaat
    82141 agatggagat atagaaagtg taatgagttt aggttcaata gttgataaat ttaaagcttt
    82201 tggttggaat gtaattgaga tagatggaca tgattttgac caaatatttg ctgctcttga
    82261 catagctaaa tcaacagtta aaaaacctac aatgatagtt gcaaaaacta taaaaggcaa
    82321 aggaatatct tttatggaaa atcaagcagg atggcatggg acagctccaa gtgatgagga
    82381 gctagaaaaa gccttgcttg aattaggagg tgctgacaat gagtaaaatg gcgacgagag
    82441 aagcatatgg taaagcatta gtaaaattag gtcaaataaa tgacaatgtt gtagtgttag
    82501 atgcagattt atcaaaatct acaaaaacac atgattttta caaggcattt ccagacagat
    82561 tttttaatat gggtatagct gaacaaaatt taataggtgc tgcttgtgga ttatcaactg
    82621 ctggtaaaat accttttgca agtacttttg ctatgtttgc tacaggaaga gcctttgaga
    82681 ttataagaaa ttcagtttgt tatccaaagc taaatgtaaa aatatgtgct actcatgcag
    82741 gtcttactgt tggagaggat ggagcatctc atgaaagtgt agaagatatt gctataatga
    82801 gagctatacc aaatatgaca gttcttgttc cagcggatgg agttgaaaca gaaaaaatta
    82861 tttttgaaat agctaagtat aatggtcctg tttatgttag gttaggaaga agttcagtac
    82921 cagttttatt tgatgaagat tataaatttg aaataggaaa aggtacagtc ttaagagaag
    82981 gaaaggatgt atcaattata gcttgtggaa ttatggtaaa tgaagctcta ttagcacaag
    83041 aaaagttgca agaagaaggt ataagtgcta gagttataaa catgtcctcg ataaaaccta
    83101 tagacaaaga tttaatatta gaatcagcaa aagaaacgaa tgttattgta acagtagaag
    83161 aacatagcat aattggagga ttaggttctg ctgtaagtga ggtagtggga gagtcttgtc
    83221 caaccattgt aaagaaagtt ggaattaaag atacatttgg agaatctggt actccaaatg
    83281 aattgttaaa aaaatatgaa ttaacttgtg atgacatagt aaagactgtt aaagaagcta
    83341 ttattgcaaa aagaatgtaa gagatataaa ttattcatgt ttgttaataa tataatatat
    83401 ttaaaagaga gttataaagt gctatattaa tttatatctc tctttttttt atatgatatc
    83461 aatgtatcaa aaatgtaata aaatattcaa atttttacaa aaatagagaa aatattcaaa
    83521 tatatattga ctattcagaa aaatatgact aatatataat ttatagagaa attaatttta
    83581 tatataatga ctacttagat aaaagaatct aaaaaattat gaaaggagga ctagggtata
    83641 aaattagatt gtaagtttta agttattata ctaaaaagct ataaaacagt tatcatgtat
    83701 tttatgttaa aggaaattgt aggtagttta tcgaattgtt taagcagaag aaaaaatttc
    83761 acatggggag gtaactgaga tatgtcagaa aaagtaaatg ttaaaacagt catcagtttc
    83821 gcaggtgcgt atgtagcaac agtaatcggt tctggatttg caacaggaca ggaaataatg
    83881 caattcttct ctttttatgg ttttgcaggt attattggtg ggataatttc aatggtatta
    83941 ttctcatgga tgggtgcttc agttatgtca aaaggtaaag agcttcaact taaagaacct
    84001 atcaaaattt atcgtgttta ttgtggtaag tatttaggaa tattctttga atggtttgga
    84061 cctttattct tatttggtgt tttcgtagta atgatttcag gagcaggagc aactttaact
    84121 gaatactatg gattaaatcc atttgtaggt agagtaggta tggcagtagt agcattatta
    84181 actgtttcat taggtcttga tagattatca aagatactag gtggaatagg gcctataata
    84241 ataatcttta cacttttagt tggtggtata agtttagcta aaaacatagg taatatagga
    84301 gaagctgcac aagttcttag cactgtaaag atagctaaac cagttccaaa tgcttatcta
    84361 tctggtgtaa tatatactac ttataacaca attgttgtta tggcattctt aactggatta
    84421 ggagcaagtg ctgctaacaa aaaagaagct atttgtggtg gtatattagg tggagtagca
    84481 cttatggctg cagctataat gatgcactta gctattcttt ctgatatagg aaacttatat
    84541 tcaaaagcaa taccatcatt attcttagca gataagattt caccaataat tggagtttta
    84601 ttctcaataa ttcttatatt aggtatatat acaacagctg tacctttatt atggtcagta
    84661 actaatagat ttgtagatga taatcatcct aagtttaaat taataacttt aatagcagct
    84721 atattaggtc ttataggtgg attcctacca tttgataagc tggttggtat tttgtatcca
    84781 tatactggat atatgggcgt cataatactg gtttgtgttc tttatagaca gattacaaaa
    84841 acatctggat atcaagaagg aatcagtgac aaataaatat tttttaatat tgtaaaccca
    84901 agagctatat gaaaacaata aaaatgtttt catatagctc tttttatttt ttagctcgtt
    84961 tattatgaaa taggaaaaac atgtttttta gttacattac atatatttat aattatggat
    85021 aaatatatac taatcaataa tttagagtct aagataagtt tataaatcat atatttagaa
    85081 tatatgggag gagtgataag attggatgta aagtttaata ttaaaggtat aatttatgga
    85141 gttatagctc ttgtattcgt tataggggga tttatattca taccaagtat gtttgtagag
    85201 aaatctaaac ccatagatta tacagtatta caaagaaatt caataccaga aaagatacta
    85261 gatgtgatgg ataagtatac aaatgaagaa agagcattag cggctaaaat cgataataaa
    85321 atatacatta tagtgactag aggaaataat aaatatggta tagaaatgga caaaatagaa
    85381 agcgtggttg aggaaggaaa ggatgttttg agagtcaaga taaagtataa agacaaggaa
    85441 gattcttttc catacatagt tgttgaaaca aatatgagtg aacttccaga tagaatagaa
    85501 ttaaatgcga cgaaataaga aggtatttta taattaaata actgcatatt gtacaacaaa
    85561 gtcatggttt ttattaatgt aagtaaattg tgtatggttc ttattgttgt aaaagccatg
    85621 acttaaaatg cttataaagt aagatattaa cagtagaatt tattactata ttaaataagg
    85681 tctggaagta cttaatgttc taattatttg gagtgataat tctagtttca gtaactatgt
    85741 agtctaattg tgcatcatga gtttcttttg gaatagaatt gaatatctgg aagtcaaatg
    85801 ctatgcctat agttatagca tcttttctta agttttctaa aaatctatca taaaatcctc
    85861 caccatatcc taatctatag caatgtatat catatgctac agctggtaca attacaacat
    85921 ccaaggcttt aatatcaatt ttaggtgatt tttcgttagg ttctcttatt ccatatgctc
    85981 cgattctcaa tccatctttt aaatctgtta tttgagtagg gattaatgta cgattttctt
    86041 ttattgtgat aggacttgat actattttct ttagagacaa aagttttttg attaaattat
    86101 ctgtttgaac ctcattgtta aaatctagat aaagcattat attagttgca tttcttatgt
    86161 cttccatttg aagtaatttt tcagttatta aatttgaatt actaagtacg aaatttgcat
    86221 ttttattttt tcttgattca ataacttttt ttctaaattc ttttttcata aaataattcc
    86281 tcccttaatg tgttataatg tgtacagcaa ttgtatttaa tataaatttt agtataattt
    86341 attgtaataa agcaatatat ttgggtaaat gtaataaaaa taacagctag tcatataata
    86401 ataatagttt atgtaaaagg agatgtatct tgtgatttca aagaagaaag ctattttttt
    86461 aggggtgatt ttaataatta taacagctat ggttacatca gcatttcagt tgacattggg
    86521 gaataaagtt gtaatatcga aagaactcta tgaagactat aaaaaatatg ataagttgtt
    86581 aggactagaa tctattataa aacaagattt ttataaaaaa gtatcagata ctgatttggt
    86641 aaatggagca tcaaaaggtc tatttctagg aacaaatgat aagtattctg gttattatac
    86701 taaagatgaa atggaaaatc taattaatga tagtgaaggt tcttatgttg gtgtaggtat
    86761 gtatataggg gcatcaaaag atggtggctt agtagtagtt cctatgaaag attctccagc
    86821 agaaaaagca ggtgtaaaat caggagataa attagtaaaa gtcaatggaa aatctgtctc
    86881 atataaaaat tcagatgaag ctgttcgtat gatgaagggt aaaaagggga aaacagttga
    86941 gctgactata ttaagagaag ataaacaatt gaactttaaa gtaaaaacag accaaatagt
    87001 tgagaagagt atagagagta aagtaattga taatgattta ggatatatag aaattaccca
    87061 atttatatca agtacatata ctgactttga taaagcttta aaagaattaa aagcaaaaaa
    87121 tatcaaggga ttagtaatag accttagaaa caatcctggt ggaatgctag atatatgtaa
    87181 agaagttgct gatgagttaa ttggtgaagg aactatagta tatacaaaag ataataaggg
    87241 aaatactgag tatttgaaat ctgataaaga aaaattggga cttccaatag tagtattgac
    87301 taatggagaa agtgcctctg ctgctgaaat attaacaggt gcaatcgttg acaataaaga
    87361 aggtatttct gtaggtacta caacttttgg taaagggttg gtacaatcag ttgtaagact
    87421 gaaggatgga acaggttaca aactgactac tgcccaatat tttacaccta atggagatta
    87481 tatcaatgaa aaaggtataa agcctacaat tgaagaaaaa gatgaaaata agcaattaga
    87541 tgtagcaact aagtggctta gagaacagat tgataaatag gatttaattc ttataaactt
    87601 atattataaa ataaagagtc ttataaaagg agtgtctcaa aatgaaattt ttagttcatg
    87661 aggcactttt ttgtaagatg tcgaatgtat tttcatcact ttaacaatga aaaagaggct
    87721 tatctctatt tttggacagc ctccataact ttatatttaa cctatttatt aactttgtac
    87781 ttacatatac catttatata agtttcgtgt atattaaagc ccttatctgt tactataaag
    87841 tctgcatcca taccttgata tatggagcca cagacattat caatattaca acttaaagca
    87901 ggtacatatg tagccatttt tattgcatct tctaaagtcg atatattcca atcaaccata
    87961 tttctcacag ccctgtccat agttaataca ctaccagcta aactcccact acttaatctt
    88021 gcaacactat tttttacatt tacattaaac ttaccaagag tgtattttcc atcctccata
    88081 gcaccagccc tcatacaatc tgtaattaaa gctatgttat tactagactt agcatccatc
    88141 attattttaa tagctatggg gcttacatga tgaccatcac aaattaattc agcataagta
    88201 tcttttagta ccatagcagc tccaaccatt ccaggttctc tatgatttaa agggctcatc
    88261 ccattatatg tatgtacaaa cactttagca cctgaattaa caacttgttt aacttgttca
    88321 aatgtagctg aactatgacc aagagaaact gagatattat ttttagttgc atattttgtt
    88381 aattctattg aacctttacg ttctggagca attgctattt ttcttattaa atttttcgat
    88441 tttttttgcc ataattttaa tatattgatg tcagggtctt gaaaatattt ttcattttga
    88501 gcacccttat atttttctgt aaaaaatgga ccttctaaat aaatcccttg tatttttgcc
    88561 ccctcaactt tattgaaact attgcttata ttttctacag aatcacttag tgtatctacg
    88621 gaatcagtta aagtcgtagg taggaaagat gttactccat attttaataa gccttttgaa
    88681 atcgtgttta ttgcttcaaa tgtattgtcc atgacatctg cacctaaaag accgtgaatg
    88741 tgagtatcaa ctaagccagg agcaatatat ttacctgaaa aatctataac attatcagta
    88801 gtactaggtc tttcttgctg aaattcaccg aatttgccat taattatact aaggtaacct
    88861 ttttctttgg ttccatgttt cataaaaaat ttatctgcat aaataaaact tttcaaaact
    88921 gcctccttat aattatgtta ttattgtgtt attgacgttt ataatatcat taagttttat
    88981 tgaaatttat ttaatttact atcttacatg ccttactcta tatacaaatt tatcgcttct
    89041 agcaacactc aaagtaaatt caatgactat attatcatca ttgtatgttg tacgaagtaa
    89101 ttttagacaa ggtgaatctc ctggtacttc taatagcgtt gcttccttgt cagatattat
    89161 tccagctgaa aattcttcat cagcaacttt aataacctca ccataatctt ctctaaatat
    89221 ttcatataaa ggtttttgtt cgattttttc ttttgtcaat cctataaatt ctttcatagg
    89281 tagatatgtt ctttcaagca tcataggaag gtcattagca agtcttaaac gcattatttt
    89341 tattattttt tcaccaggag ttatacctaa tttttcagaa aaatactttg tactttctac
    89401 tacctcaaat gataatactt ttgtacttgg cttttttcct tgtgttctca tataatctgt
    89461 aaagctataa ctttccatta agttttgaga attttgactg agtgcagcaa caaaagttcc
    89521 ttttccatgt cttttatata tatatcccat atgttctaac tcagccatag ctagtctaac
    89581 tgtagttcta cttactccat attcatcaca aatttttctt tccgaaagca ttctatcgtt
    89641 tggttccatc tcattttcaa tcttagttct taatatttca acaagctggt catatattgg
    89701 tcttttatca tttcttttta tcataaaaat atagtccttt ctaagtggtt attaccacaa
    89761 tttgattata gaatatagaa ttatttatgt caatgaataa agtttaatca aaactcaata
    89821 agtaagtcta ttaatttatt ataaaattat ggtacattga caaaacaagt aaaataattt
    89881 atattcaaat tggtaataac cactttgggt atattgtaga cgaggtattt tttattttac
    89941 atattaattt attgtttaga aaagtaaaaa attaataatg aggagaatct agtatgatta
    90001 cagtaataac atttaaccca tctatagata gaatgtatag ggtaaataat ataaacatag
    90061 gtgaggtgca aagggtagta agtaagaatg caactgctgg aggtaaagga ttaaacgtag
    90121 ctaaggtatg taaaatttta aaagaaaatc ctttagcgat aggatttcta ggtggattta
    90181 atggagaatt tataaaaaat gaacttaaaa aattagagat acaaaataag tttacaaaaa
    90241 tagagcagga aactagaaat tgcttaaata taattgagga caataaagtt agtacagaat
    90301 ttttagaaaa aggaccgata gtagaaaaaa gcgatttaga gagatttgaa aatgatatag
    90361 aggaagtatt aaaaaatact aaaatactag tggcatctgg tagctattgt gagaatatgc
    90421 cgatagacta ttatgaaaag ataggcaata tatgtagagc aaataatata aaatttatat
    90481 tagatacatc aggagaagca ttaaaaatag ctttaaaatc aaagccgtat ttaataaagc
    90541 caaatataga tgaaataaga catttattag gaataaatat agaatcaaga gatgaaatta
    90601 tcttatcagg aaaaaaactt attgaaatgg gtgcagagaa tgtatgtata tccttaggaa
    90661 aagatggtat ggtgtattta aataaagata gtgtatatga tgtaaaagta ccagttgtaa
    90721 agtgtataaa tactgtaggt agtggagata gcacagtggc tggatttagt gtaggtattc
    90781 taaggggtta taaaatagaa aatttactta aatttgcaaa tgcttgtggt ataagtaatg
    90841 ctttaaatat ggaaactgga tttgtagatt tgagagaaat tgataaatat caagatttag
    90901 ttgaagtaaa aaagttaagt taagtatatt taatgtgaat atactaagtt taatgttgtt
    90961 gtaatagatt taaaatttaa tataaaagat atagtcttat ataaaaatag gttagagaaa
    91021 ttcatcttta acctattttt ataataaata tctattttac tgaatttata attttttcta
    91081 gtaatctttg agccttgaca cactcttctc cactaatagc tggttttata ttattttcta
    91141 tacaatcaat aaaatgattt gatatatctt caaaaccctt ttgttttaat atatttaccc
    91201 atgcaccact ttgttttaaa attaggtttc caccttcttc tatttctagt acattaagat
    91261 tcttaattct ttgaatcctg ccttttgaaa gaatttcaac ttgttctaac tttgttccag
    91321 aatctctatg catagaggtg tttatagaga aatgtttacc tttaagttta tgacttacaa
    91381 atataagatt cttattgtct gttaaaaata aatcttctcc actaatttca acatcttcat
    91441 ttgctaacca taaagcagtg tcaataacat gtatataatc atctataagg gtactgtcga
    91501 atctaacatt tcttaaggaa tttaatccat gtttacatat attgatagat acgatttctg
    91561 tagcattatt tttaatttct ttgtacatag gacagaatct acggttgaat ccaaccatta
    91621 aatttaagtt ttttttcgta cttaattcga taagttcttc tccttgattt actgttgaag
    91681 caagaggttt gtctacatat acatgaacac ctaaatttaa taatattttt ataatttcgt
    91741 agtgtgtttc tgtagagcta tgtagaaaaa tgcaatcaca ttttttagct aaagactcaa
    91801 tagaatcgaa tggcatgatt ctataatcac tacagatttt ttctctttta actttatttg
    91861 gagtaaaagc tcctacaaac tcaaatctct cagatttagt taatataggt aaatatgcct
    91921 tttgagctat ggagccaaga cctatcattc ccatttttat atttttcata atactttctc
    91981 ctaaggtttt tatatttaca gaattgatta taattcagac acgtaaattt gtaaactttg
    92041 tatcaaaaaa taaacttaag gcttatataa gtaaattagc aaaataagaa atattaataa
    92101 aataattttg taggatatct ataaaattga tttatataaa tattttatct attgacaaaa
    92161 aattcaaatt aatttatatt tggattagtg gtaattacca ctttggtctg aacaagtttt
    92221 tatcgtaaaa taaaaatact aaagtatcta gagtatgatt ttattaaatt tttaaagaca
    92281 aactataaaa ataatcaaaa tatatagagt atattttata gtgagtttaa gtagtaataa
    92341 aaaatctagg caagaaatta aaagaagggt atattttttt ataccttaaa gtggttataa
    92401 cctcattacc aaaaataaat gttgtaagta tgaaaggaga atgaaaatgt ttaagttaga
    92461 agaacaaaaa ctaaaagatt taggagcaat aataacaact aatgaaataa aacaacaacc
    92521 tgaattatgg ttagaaactt atgaaatcta taaatctaac aaagaagaac tacgtagatt
    92581 tatagataaa ataagtaata atcatggaca gtttagagtt atatttacag gtgctggaac
    92641 ttcagcttat gtaggaaact caatactacc ttatcttaag aataacaatg acataagaaa
    92701 atacatattt gaagcaatac ctactacaga tatagtttca aacccatatg attacttaaa
    92761 aaaagatgta ccaacgttat taatttcatt tgcgagaagt ggaaatagtc ctgaaagttt
    92821 ggctgcttta aatttaggaa ataagattgt agataatttc taccatttag ctataacttg
    92881 taatccacaa ggagaactgg ctaagatgac taaaaacgac gaaaataatt acttgttatt
    92941 aatgccatca aaatcaaatg atgaaggctt tgctatgaca ggaagttttt cgtgtatgat
    93001 gttatctgct atgttaatat tcgatagctt agaagatgat gtagaaaagt catatataaa
    93061 tgcaataatt gaaatgggaa gaaatgttat agatagaaaa gatgaaatac atgagttaat
    93121 aaataaagat tttgatagag ttgtgtattt aggttcagga ggattaggtg gtttgacaca
    93181 agaagcacaa ctaaaacttt tagaacttac agcagggaaa atagctacgg tgtatgattc
    93241 tcctatgggg tttagacatg gaccaaaatc atttattgat gaaaatacac ttgtatttga
    93301 atttgtatct aactgtttat atacaagaaa atatgattta gatgttttag aggagataaa
    93361 aagggataaa attgctaaat tcacttgtgc agtgtcagta gaaaatgaaa ataacttctc
    93421 aggaacaaag tttgagttta aagaaaagta taataaatta ccagatgtgt atcttgcaat
    93481 gccatacata ttgtttgcac aaactatagc attgtttgta tcggtaaagg ttggaaataa
    93541 gccagatacg ccatcagcta caggaacggt aaatcgagta gtaaaaggtg taacaatata
    93601 tgaatattag aagagaagat taaaatatta taactaaata atttgggagg gtattaatgt
    93661 ggcattagta acaacaacat caatgcttaa aaaggcacaa gatgggggat atgcagtagg
    93721 agcatttaat gtagaaaata tggaaatggt aattgctgtt atagaagctg ctgaggagtt
    93781 aaattctcca gtaatattac aaactactcc atctactgta aaatatgcag gtctcgatta
    93841 ttatctagca aatgcaaagg ttgctgcgga aagagtaaag atacctgtag caatgcatct
    93901 ggaccatggg tcaagttttg accttgcaat gaaagcttta agaagtggat atacatcaat
    93961 aatgatagat ggttctcatg aaagctttga aaataatata tctatatcta aatcagtagt
    94021 aaaagcatgt agcccttctc ttataccagt ggaagcagag cttggaaaag ttggaggaaa
    94081 agaagatgat ttagatggag ggaatagtaa tgattataca aatccaaaag aggcaaaaga
    94141 gtttgtcgat agaacaggaa tatcttcatt ggctattgct ataggtactg cacatggatt
    94201 gtataaggga gaacctaaaa tagatttaga taggttatca gaaattaaaa atgtagtttc
    94261 aataccttta gtattgcatg gaggttcagg aatacatgat gatattataa aagaagctat
    94321 aaaaagaggt attgcaaaag ttaactatgc aactgaatta agaattgcat atagtaaagg
    94381 ggttaaaaaa gttttaaatg aagattctga ggttatagac cctaaaaagt atggactagc
    94441 agggcttcaa tcagtaaagg attttgtaaa agaaaagatt aaagtatgtg ggtctgtaaa
    94501 taaagtataa aaaataaaat atcctaaatt tattttagat aagtttagga tatttgttat
    94561 atgaaaattt tatattaatt atgggtaaat ggtgtatttt tttcatttaa ttgatgaaaa
    94621 atatcagctt ttattggtaa agtattacat atataattaa aatataataa aaattgaact
    94681 atatatttta ttattatgaa atatggatat taaattttag tgctaaaatg tacgaggata
    94741 taatattcta taaatgaaaa caaaaggttg actttataag aaaaaatttt ttatgctact
    94801 ttgaaaaata caataataac actgagagag gtggggatat gagagaacgc attattcttg
    94861 aagcattaaa aaatagtagt ggttatctaa acatagatta ttttgcagat aagttggggg
    94921 tatctactcg aactatcaga aatgaaataa aaaagataga aactataaaa gaatgtaatg
    94981 gtttcaaatt agaatataag tctaaactag gatatattct aaatattaaa gaccaagata
    95041 aattcgagca ttacctaaga aacttaccct cagatttagt tgaaaatcca gaacaacggt
    95101 tagaatctat aatagtagaa cttttagtaa atgaaggtta taaaactata gaacagttaa
    95161 gtaaaaaatt tttagtaagt tcatctcaaa taaaaaatga tttaaaaaag atagatgaaa
    95221 aaataaagga tacagaatta aaattggagc gaaaggctca ttatggaata aaaatagaag
    95281 ggtctataaa agaaattcaa agtatactgg tggatagtta ctttaaaggg aatagaaata
    95341 taacaaaata cagaaataaa tttatagata atattaaact agataatatc agaagtacaa
    95401 taaaaaatgt acttaatgaa cattatttag aagcaaattt aaccgaactt gaggaaatat
    95461 tagcacaaat tattatattg tatataagag ttagcactag agtagtaaga agtcctagtg
    95521 atttagaatt atatacagaa gatttaatta tagacgaatt attagataaa atttttggag
    95581 ataagaagta tagccttact tatgatgaaa agtattattt aaagcaactt ataaaattaa
    95641 ggactaagga taagaaagcc accataaaga acattgataa gaataaactt caagatatta
    95701 tgtgcgaatt ttttagagat atcgataaaa agtataactc taatttttta gaagataaag
    95761 agttccttaa cttattttat ttacatattg catgtttaat agaacgtata aagaagaatc
    95821 acaagataac aaatcctttt tcagttaaaa taagtcaaca gtatccaact gtttttaatt
    95881 tatctattca attttcaaaa atcatagaaa atgagtatca cataaaaata agtcaagatg
    95941 aaataggttt tatagctact cacattgctg ttccttttga aaagagagaa gaggctaatt
    96001 ttaataaaaa atataaaata gcaataatat gctcttctgg tggaggcagt gcatttttga
    96061 taaatttgag acttaaagaa atctttccaa atgctgaaat aaaaaacttt tcattactag
    96121 aagaaaaggc tgtaatagaa tttgcgccag atttgatttt ttcaataaca aatcttttat
    96181 ttgaaataaa tgctccagtt atacttataa atgaaatttt agacgaactg gattatttgg
    96241 atattaaaga aagttttaga tttgtagacc atataggaaa tgtatcaagc ccaaaacaat
    96301 atatattagg tctgtttgat aagaatcact ttaggtgtgt aaaagagaaa accaaatata
    96361 aagatatcct tgatagtatg tcgcaatgta tagttgatga gggagcttgt tctccgactt
    96421 atcccaaaga tgtgtgggaa agagaaagct atttgagcac tatttacaca aatggtgtag
    96481 cgatacctca tccaattgaa atgacaggta ataaaaatat tatatcagta gccttgatac
    96541 aaactgatat taattatgaa aatagggtac caaagattat tttcatgatt tcattaataa
    96601 agggaaatct agagcttcat aagcaaatat ctaaatattt aagtaagatt atgacagata
    96661 aggatatggt agatatgcta aataagtcac aatcttatga ggaatttatg tataagctaa
    96721 aaatctatct gggaggttag acatgaatat agagtttttt aagaggttat cagaagcaga
    96781 tggtatagca tctaatgaag aagaggttag acatgtttta ttggaagaac taagagctta
    96841 tagtgataaa gttatatgtg atggtctagg gagtataatt ttttctaaga taaaagatga
    96901 aagtgcacca aacgtaatga tttgtgctca tatggatgaa gttggattta tggtgagaag
    96961 tatagacaaa cttggtatga ttcacctaat gacaatagga ggagtaaaac ctcttgcaca
    97021 gtttgtacaa aaagttagaa ttacaacaaa agaaggaaag aaaatccacg ctatcataaa
    97081 tgctacatat aataatggta aggctgaaaa tatatatgca gatattggag catatacaga
    97141 agaagatgta tacaacctag ggattaatgt tggggatatg gtaacatata caacaaactt
    97201 tgaggaattt actttgccag accgattagt gggaaaagct tttgatgata ggataggatg
    97261 ttttgtgatg ggagaagttt taaaggaact taagaaagaa aatctaaatt gtaatattca
    97321 ttttgcagct acaagcagtg aagaagttgg aataagaggg gcaaagacgt caacgcagtt
    97381 aataaatcca gatatagtat ttgtaataga tgtagcatgt gctaagaatg agtttgtgag
    97441 ggactataca aatcagaaac aaattgataa aggtgtaatg ctaatgcata gagataggac
    97501 tttagtccct agtagaaaaa tgatagatta tgtaatgaaa attgctagtg aaaataatat
    97561 accacttcaa catgatatgt ttgaatcagg tgggactgat ggaggagaag cacatctcgt
    97621 aaatgaaggg aaaccttgtg tagtaacttg tgtaccagtt agatatggtc attgtgcatt
    97681 ttcaatagta agtaataaag atttggaaaa cattataaga ttgtatacac agttaatttt
    97741 aaattttgat gaagaaaaat atagacatat aagaaatttt atctaaagga gaataagaaa
    97801 atgtcaacag aagtacaaat aatggaactt atatcaactg caggagagag taaatcgaaa
    97861 gcttttgagg ctttaaaaaa agttaagtca aaagatttta aaggagcaaa attgttaata
    97921 gaggaggctc gtaaaataga tattgaagct cacaggatac aaacagaatt aataactcaa
    97981 gatttaaatg aagacgttga aagcccagca atgagtcttt taatggtaca tgctcaagac
    98041 cactatatga cttctcagct agcaaaagac ttgatagagg ctttgatata ttgtcttgat
    98101 aagtaattgt aatgtagaaa tagattattt atgtaaaaat tcttttaaaa gtttagatgc
    98161 ttttaagaga taataaaaat acaaacttta ggaggaatta ttatgaaaat attattatgt
    98221 tgtgcaggtg gattttctac taatatgtta atgcaaaata tgaaaaaggt ggtaaaagag
    98281 agtgctaaac tcaatgaaaa agattttgag tttacagcta taccagcaga ttcaatagaa
    98341 agtgaagttg atgactggga tatagtactt atagggcctc aaatagctca caaaatagat
    98401 ttcttaaaag gaatattaga gccaaaaaat aaaccttttg cagtaataga taaagatgta
    98461 tatggaaaaa tggatggagc tacagttatg aagttggcat taataactta taagaaacat
    98521 caattagctc aaggataata aaaataatga gggaaaaggg agattagata tgttttcaaa
    98581 atttgaagca tttatgaata gagtatttat gcctttagct cataaggttg acaatcaacg
    98641 tcaccttggt tctataaagg ctggtatggt tgctatgaca ccattcacaa tattagggag
    98701 tatatttgca atacttccag cacttccaaa tatgcttgga gaagataatc caataagcaa
    98761 attcatactg aacaattcag caatattgga cttaccagta agtctttcca taggcttaat
    98821 aggattatat gcttgtatgt gtattgcttt taatctgggg aactactata aattatatat
    98881 accaggttgt gttgcactat caactttttc ttttttattc ctagtagcat catttacacc
    98941 agatggagct ttagaaatag gaaatttagg agctaaagga ctatttacag gtatgatagc
    99001 agggcttggt tctgtggagt tatataattt ttgtaataag aagaatatca caataaaaat
    99061 gccagaaaat gtacctgatt ttgtatctag gtcttttgaa ttaataccta ctactataat
    99121 agtttgtgga acttttatag ttgctcgttt tatttcatta aaaatatttg gagaattacc
    99181 tccacagata cttactcaat ttttatctcc attagtagga agtatggata acccatggtc
    99241 agttcttgca cttaactttg ctatatgtac tatattcttt tttggaatac attcatcagt
    99301 atttagccct ataactagac ctataatgat tacatttata gctgaaaata tagcagctat
    99361 gcaagctgga gaaacaatac ctcatttctt tacagcagga gcttcaagtg cattctttgg
    99421 atttacagga tgtggaatta gtataggttg tgtaattgca tgtatgttat caaaaaatgc
    99481 ttcctataag aagattggac gtgtatcatt attccctgca ttatttggga taaatgaacc
    99541 tatattattt ggagcaccaa taatattaaa tccaataatg tttataccac atgtatttgg
    99601 tggagctata ataggtacac taccaatgtt ttttatgcac tggggatggt tagcaaagcc
    99661 aatatttgac cctccatatg taggagtatt cttagaagga tttttaacta atggagattg
    99721 gcattctata ttagcaaatg cattgcaatt agtattaaca gttttattat attggccatt
    99781 ctttaaaata atggaacgtc aacaaaataa taaaaaacaa aaagaagaaa ctaagaatat
    99841 attctctaaa gaagaagaag acttattgga tgatttagga ttagatttct aatattttgt
    99901 tatgaataat acaatagaga gactagtatc cttaatcgat aaaagaggtg ttgacgctct
    99961 tttgattaaa gcaaaaaata ctaaaagata tataggggca ttatcaggta gtggtgtata
   100021 tgtgcttgtt actaaagata aaaattatca agttctagat ggcagatata ttgatgaagc
   100081 agataaaaaa acgactggtt ttataaaaag gattgtatct caaggttctt atataccaac
   100141 tataattgag ttattaaaag aattaaatat aaaaaagtta gcgatagaaa accaagctat
   100201 aagtatacaa gagtatcttt cactaaaaaa tgcaggtttt aaaatcaatt taataacaaa
   100261 tgaattatgg aagctaaggg ctataaaatc taaagaagaa attgaactta ttaaaaaggc
   100321 ttgtgagatt acagatgaag tatttgatga ggttatttct gaaattaaaa aagatatgac
   100381 tgagctagaa gtatcagctc taattcagta tcatgcattg aaaaaaggtg caagtggaat
   100441 gtcttttgag acaatagtgg tatcaggaga aagaggtgca atgccacatg gaagacctac
   100501 aaacaaaaag ttgaaagtga atgaagctat aacaatagat tttggtgttg tttatcaagg
   100561 gtatcaatca gatatgactc gtacaatatc tataggtaaa cctcctaaaa ttattaaaga
   100621 gatatatgat gtagtattgg aagcacaatt atctgcaata gagtcaataa aggaaggaac
   100681 tagggcaagt gatgttgaca aagtggctag agaaattata gataaacatg gatttggcga
   100741 atattttaat catggactgg gccatggtat aggtcttgga gatggtgaag ttcccactct
   100801 aaatccaaat agtgaagata tattagtgga gggaatggta atgtcttgtg agccaggtat
   100861 ttatattcca aatgtagggg gagttagaat agaagatgac attgttataa tagatggaaa
   100921 aggtgttcca ctcaataaga catccaaaga gtttataata ttaggagaat aaaattatga
   100981 aatataattt taataaagta ttaaatcgta aagaaggaaa ttgtaggaaa tggagcaacc
   101041 acgtaattaa agaaaaattc ggcttaaatg aagatgcaat tcctatggat ttagctgata
   101101 ttgattttga gtgtgctcct gctataaaag aagctatctt aaaaagagca tcaataggag
   101161 attatagtta tacttttata ggagatgact tttatgatgc tattataaat tggaataaaa
   101221 gaagatttga tgtatatata gaaaaggatt ggataaaatt aacttttggt actgtttcaa
   101281 cccttcatta tatagtccaa gcatatacta aagaaggaga aggtgtattg ataaatactc
   101341 ctgcttatga cccttttgca gaggctgtat taaataataa tagaaagttg tgttgtagct
   101401 cattaaaact agataacaat agatattatt tagatttaga ggatatggaa aatcaaatga
   101461 aatacgagaa tataagattg cttatattct gtagtccaca aaatccatca ggtcgtatat
   101521 ggacaaaaga agaactatat caagtgtcag agttatgttt aaagtataat gtaattttgg
   101581 taagtgatga aattcataga gatataatat ttaaagatta tagatttgta tctttgtgga
   101641 atgctcatcc agaaatttat aaaaatagta taatctgtgt atctcctaat aaagggttca
   101701 acttaggtgg attaaaaaca tcatatgtat taataagaga tttaaaaatt agggagactt
   101761 tattagaaag gctaaaaagt aattcaataa cttctccaaa tgtatttgca ataccagcaa
   101821 tcgttgcagc atacaatgaa agcgaagaat ggctagatgc tatgactagt tatgtagagg
   101881 agaattttga aatagtttat gatttctttg aaactcatat accaaaagcc aatgtgatga
   101941 agtcagactc ttctttttta gcctggatag atgtaagagg agtgtttaga aatgaagaag
   102001 aatctaaaga attttttaga catgcaaact taactatggt agttggaagt tattttgtga
   102061 aagatggaga tggatttatt cgtattaatg tgggatgtcc tagagaaaca ttaaatgagg
   102121 ctctaaatag aatcaaaaaa acgtatataa gtatgtattg ttaaattata aaaaaggtat
   102181 atatggttta tataaaaaca cattatttag ttatcaaata ttactggaat tttgcattcc
   102241 agtaatattt tttgattaag gaatattatt gaagaatatt aaataatatt atttcatatc
   102301 cttatattaa cttatataaa aatattttat tattttggga acgggttagc taacctttat
   102361 ctagtttata tttatactgt aagaagcagt aaaaactttt cctttactca gtttattaat
   102421 aaacttctta tctttataaa ttttattaga atttatatta tcagcaagac cataccaagg
   102481 ttctatgcat aaaaaaggag cagtagaatt ggtttcacta taataaggag tccaaatacc
   102541 aactagaggg aaatctttca tagatatagt tacatattta ctaccattat tattacatat
   102601 agatacttca tctatgttgg tgtatatcaa agcatcattc ttgaataatt ctggatttaa
   102661 ctgtaagttt tttaattttc caatagatat tatatcatcc gtaaaagaac cacttaaatt
   102721 gattttttct acatcatttc tacttttaaa ttcaaggtag tgtttagaaa aatcattttg
   102781 ttcgcaagaa ggaaggttga atgcaggatg tccacctata gaaaagaata tatctttaga
   102841 gtcagtattt ttcacaatcc attctacctc aatgctgtta tcatttaatg tatagtttat
   102901 gattagctca aaagaatatg gatatttctt taaagtagat tcattatctg ttaatttata
   102961 agatacgaat gtatctcctt tatctacaat ctcaaagtcc atatctctag caaatccatg
   103021 ctgagtcata ctatataaat tttcttctat tattgtttca ttatcaaaaa gtttacctac
   103081 tataggaaac agtataggtg attgtctacc ccaatatttt ttgttaccat tccaaagaat
   103141 atttttgtcg tattttttag aaaatatttt agttagttca gcaccaaaac tgcttgattc
   103201 gataattaaa ttttcatttt gtaatatatt catttaaaat ctcctttatt atattttatg
   103261 agtatattat actataaagt ataaagatta ttaaatttta tttgatatta tatttgaaag
   103321 gtagatttta gtatattttg aaaattttgt tttgaaaatt tgggattatt taagaaattt
   103381 tgtattataa atagaaggaa atagataaat tgtatagaat aaaatatatt aagataagtt
   103441 aataatgtta aatgggaagt gagtttatgg aaaaaccaaa tacaaaagtt gttaactttc
   103501 aagagtataa agacagaaaa aacaaaagag aagtaaatag agacgaactt ttaaagctta
   103561 taaaaaaaat tgtaacttca aaatagggag ttgttaaact ccctatttta atgggtttca
   103621 taataattaa gacattctaa gtccatctat ataatctttg aattgtgaga aatctgattc
   103681 actacttaat tctatagttt ctgaaccttc cgtataaaaa ttcttaagat agatagtata
   103741 attattatca acacaatcat gataaataca tctatatcca tcttcataca agctttttaa
   103801 cttagaaaaa tcatccacaa tatcatctcc ttaaagttct tttaaatata tttttacaaa
   103861 aagcatctaa ttgtatgcaa attgaaaatg taaaataaag gaaatgaata taaaatgtag
   103921 aataattttt atatgtatgt aaaaataaaa agaaaaagtt aaaattttta gaaaataaga
   103981 gtgtttttta tctaaaaaga gagtttaaaa aataaaaaaa tgttgggagg ataaaaaatg
   104041 agtttattag aaagtatatc aaaaaacata tattccctag atagttcttc tattgaaaaa
   104101 acaaagcaga gattggatag gcttatacat ccaactggaa gcttaggaaa aatagaagat
   104161 atttgcatgc aattagcagg aatatttggt aatgaaaatt ttgatacgag caaaaaggtt
   104221 ataattgctt ttgctggaga ccatggtgta tatgaagaag gagtagcacc tgaccctcaa
   104281 aatataacaa aactacaatt tccaaatttc tctaaaggcc tatgtggagt tggagtaata
   104341 agtaaatttg taggggcaga tgttgtagct gtagatgtag gaataaactg tgatgaaaaa
   104401 ctggatggag tattagatta caagataaga aaagggacat caaatatggc aaaaggtcca
   104461 gctatgagta aacaagaagc tattagatgt ttagagatag gtattgaaat agcagaacag
   104521 tgtatagaaa aagattataa ggttattggt attggggaga tgggtatagc aaatactact
   104581 ccgagcacag caataatatc tgtaatttca ggttgtgacc cgttagaggt tactggtata
   104641 ggtgcaggtc ttaagaaaga aagactaaag cataaggctg aagtaattag aaaggctatt
   104701 gaaattaaca atccaaatcc aactgatgga gtagatattt tatctaaagt aggaggattt
   104761 gaaataggct caatggcagg tgttatacta ggttgtagtg ctaataggat tccagtagta
   104821 attgatgggt ttatttctta tgcagcagca ttaattgcat ataagataaa tccaaaaact
   104881 agagaatata tgatagcatc tcatttatca gcagaatcag gaactaaaag agcattagac
   104941 atattaaaat tagaccctct attaaatatg gatatgagat taggtgaggg aagtggagct
   105001 gctttagcat ttaatataat agaagcttca aattatactt acaaaaatat ggcaactttt
   105061 gatgaaattg acatgggaag ataaatataa aaatttaata tgtaggagtg ttttgatgag
   105121 taaaattatt ctagtaacag gaggcgctag gtcagggaag agtaactttg cagaaaaact
   105181 atgtttggac agaaataata atacagctta tatagcaacc tctattccat ttgatgatga
   105241 aatgaaagat agagtaaaaa aacatcaaga gaatagacct agaaactgga atacctatga
   105301 aatatatgaa gatatatatt ccataattaa agagatatat aaagaacatg aaactgtcat
   105361 actggattgt gtaacattat tagtaaataa tctaatgttt aaacacaatt taaatataga
   105421 agagtctagt caagaagaaa taaacaaatt agaacaatac ataaaagaac aagttgtcaa
   105481 acttattgaa gaaatagaaa aaacaaattt atactttgta tttgtaacga atgaattagg
   105541 tatgggaatt gttcctggga ataaacttag tagaatttat gctgatattg caggtcgcat
   105601 aaatcaatat atagcgtcaa aaagtaatga ggtttatttt gtagttagtg gcatacctat
   105661 gaagataaag gagtagtatg tgattatgaa aagatttatt ttaatactac agtttttgac
   105721 aaggatacct ataaaattaa atgtaggatt tgatgatgaa ttttataagt caatagtata
   105781 ttttccactt gtaggttttg tcataggtat attatcctat ttagtaggat ggatttctat
   105841 gcttttattt gaaccattta tagcttctat aataataact ctagctggcg tcttaattac
   105901 tggaggactt catatagatg gattaggaga tacatttgat gcaatatata gttataggaa
   105961 taaagaaaaa atgcttgaaa taatgaaaga ttcaagatta ggtacaaatt cacttctagc
   106021 aattatgttt gtacttttat taaagattgg attcatctac gatataataa gtaataattc
   106081 attgtgggtt ataattttta tgccaatgat tgcaagatta ggagtaatgt tattgacata
   106141 taaaactgtg acaccaaggg aaaaaggaat gggaaattta tttataggaa aattaactac
   106201 aagtatgtta ataactgcta taatatacac attactgata gttgctctta ttactaaatt
   106261 tatattttta ttacctaata tagtgttgat taaagtatta ggttcaacta tagtagtttt
   106321 tgtatttata atattattca aaaaacatat ttataaaaaa attgacggag taacaggaga
   106381 tatattaggt tgtggaatag aactttcaga ggttgtatat ttaatttaca tatatttgtt
   106441 aaattttatg tttttttaaa gtaaaaagtg gtgataatat gataagatta atattaataa
   106501 gacatgcact aacaaatgat aataaaaaag gaaaattatc aggtcatata aatagttgta
   106561 ttagtgaaga aggtaaacta caaataaata aaattactag atatctgagt aacgaaaaca
   106621 tagataagat ttatacaaca ccatctacac gtactaaaga tacaatagaa aaaatatcaa
   106681 aattgaaatt attagaaata gaagaaaaag aaactttaag agaaattagt tttggagatt
   106741 ttgaaggaat aacttttgaa gaaattaaaa tcaaatatcc caatgagttt gaaaaaatga
   106801 taaaagaagg taacaactat aggtatccaa atggagaaag tttgatagac tcttataaaa
   106861 gagtggcaaa agaaattgat aatataattt tagaaaatga tagtaattta ggttcaaaga
   106921 caatacttat atgttctcat gcaggaacaa taagaaatat aataacacat ttgatttctg
   106981 gtagctataa gtatcattgg aactttaaaa ttgataatgc gtctattaca gttttagatg
   107041 ttgatagtgg atttactgta atagataaaa tgaattttac agacttcata taatttaatc
   107101 aaaatcaaat attacaatct gtgtttgtaa tatatctgta aaattagcaa tgaaaataga
   107161 atattagatt aaaattaggt tcttaattag atgttaaaag ggaaaaaggt tagatgcctt
   107221 tgcagccccc gctactgtga aaccaacgaa actataaata ccactgtcag tttgatggga
   107281 aggttataga agtaggatga agttaagcca ggatacctgc ctaatttaat ttaaaacaag
   107341 aggtcttcgg ggtgaaagat ttttaattaa tatatataaa agtcctatct ttagaagcat
   107401 taggactttt tttaatttgg tttttagtaa attttaacaa ggagggatgg aaagtgggga
   107461 aaaaaataat gctacaagga acagcttcaa atgtgggtaa aagtgtctta gtagcaggtt
   107521 tgtgtagaat atttaaacaa gatggttata ctgttgcgcc ttttaagtca caaaatatgg
   107581 ctttaaattc ttttataact aaagaaggat tagaaatggg aagagcgcaa gtatttcaag
   107641 cagaggctgc gaaaatagag ccaatagctg atatgaatcc agttttatta aaaccttctg
   107701 gaaataataa aagtcaagtt attgtaagag gtaaagttgt aggagaaatg ccttcttcta
   107761 agtatcatga ttataaactg gagttagtag atgtattgaa ggaaaccttt aatagcttaa
   107821 attcaaggta tgatattgtg gttatggaag gtgcaggaag taatgcagag ataaacctaa
   107881 aagacagaga tatctctaat atgggtatgg cagaaatagc tgatgcacct gtaataatag
   107941 tcggagatat tgatagagga ggtgtgtttg cctcattagc aggaacaatg cttttattaa
   108001 atgaagatga gaaaagaaga gtaaaaggtg taataatcaa taaattcaga ggtaaaaaag
   108061 agcttttaca agatggtgta aagatgctag aagacatcat aaaagtacct gtcataggag
   108121 tagtacctta tacagatata aaaatagaag atgaagatag tgtaactact agatttaaga
   108181 aaaaaatgaa taaaagcgat atacacatag aaataattag aactccacat atgtcaaatt
   108241 ttacagattt taatatattt gagacacaag aagatgtaag tattagatat gtagactatg
   108301 gagaatcttt aggaaatcca gatatattaa taatacctgg aactaaaagt actatagacg
   108361 atttaacata tataagaaaa agtggtcttg aatcgcaaat caaaaattta catagtcaag
   108421 gtaaattaat atttgggata tgtggtggat accaaatgtt aggtaaaaaa ttaaaagacc
   108481 cttatcatgt tgaaagtgaa attgaagaag ttgatggaat aggtctttta gatacagaaa
   108541 caacgtttga gaaagaaaag actacaacac aagtagaagc cactattgtt gaatgtatca
   108601 gtgaatatat gaataacctt aaaggaaaga aaataaaagg ttatgaaatt catatgggca
   108661 ttacaaagcg aggtaatgat gtaaggagtt tagatttaat aactaaaaaa cttgataaag
   108721 aagtgaatta tactgaagga agtgtaaata aagaggggaa tgtaattggg acttatcttc
   108781 atggaatatt tgatgaaata agttttacaa gagcaatttt aaacaatata agaaagaaaa
   108841 aaggtttagg agaattagaa agcaaagtaa actcttttaa tgaatttaaa gataaagagt
   108901 atgataagtt ggctgatttt ttaagagaac atttagatat agagaaaata tatgaaatta
   108961 tgaaataatg ataaccttta tttataattt aggtaggagg tgtattatga aaaaaatttt
   109021 aattgcgggt actagtagtg gagtaggtaa aacaacaata tcattaggaa taatgcaagc
   109081 tttggttaag agaaatatga aagtacagcc atataaagta gggccagact atatagaccc
   109141 ttcatatcac acttttataa ctggaagaca ttccagaaat ttagattctt atatgttaga
   109201 tgatgaaaaa ataaagtata tagtaaataa gtcctcaaaa gatgcagata tttcagtaat
   109261 agaaggtgtt atgggcttat atgatggttt tggaatagat ttagataatt gtacaagttc
   109321 acatacttca aaggtattaa agtcacctgt tatattagtt ataaatggta aatctatggc
   109381 agcatctagt gctgctatgg tacttgggta tagggaacta gataaagatg taaatataaa
   109441 aggtgttata gtaaataatg taaagactaa tagccattat caaataatta agagttctat
   109501 agaaaaatat tgtaatgttg aagtattggg gtattttcct ccaaataata aattctcttt
   109561 agaatctcga catctaggat tagttcctag tgtagaaatg aaatcactaa gggaaaaatt
   109621 ttatacttta gcagatgaaa ttgagcacta tatagatatt gatagaataa ttgaaatatc
   109681 agaaagtgat gaatttgaga gtgattttga ttttcaaact ttgatagaaa ataataaaat
   109741 aaaagaacat gttaataata aatcaatagc tatagcatat gataaagcat tcaactttta
   109801 ttatagagaa aatatagagc ttttagaaga acttggttta gaaataaact attttagccc
   109861 tcttgaagat gaatcagtgc caaatgcaga ttatatttat attggaggag gttttccaga
   109921 aatatttgga aaagaattag aagcaaacga gtcaatgaga gtttctatat tagaagcaca
   109981 taacaataat atacctattt atgcagaatg tggaggactg atgtacctag gtgagaaact
   110041 attgaatcaa gaagaacaag aatttaatat ggttggtata ttcagtggaa ttagcaagat
   110101 gacatcttct ttaaagagat ttgggtattg cattggtgta gccaaggaaa atactctttt
   110161 atctaaaaaa ggggaatcta ttaaaggtca tgagtttcat cattcaatat ttgaaagtga
   110221 tgaaaaatgt gtgtattcaa tgagaaaaga acgagatgga aatatagtag aagagtggga
   110281 tggtggttat agtaaaggaa acactttggc tacatatctt catactcatt tttataataa
   110341 cttaaattgt atagaaaatt ttttgaacaa aggaaatgag taaatgaata ttttatctat
   110401 ttatataggg tatgtgacag acttaattat aggagaccca tattcttttc ctcatccagt
   110461 gagatttatt ggtaaactta taaattttac tcaaggtata ataagaaagg tctctaaaaa
   110521 tgataggcag ttaaaattgg gtggatttat attatggttt ataactgttg gaataacata
   110581 tttaacaact tatgctatag tgaagctatt tagttttaat atattattga gtattgtagt
   110641 gaatagtttt ataatttata caacactagc cacaaaatgt ttaaaagatg aagcattaaa
   110701 aatctataat gtattaaaaa ctgaagatat agagaaatct aggattcagc tatcttatat
   110761 agtaggtagg gataccacta atttaagtga acctgagata ataagagcaa ctgtagaaac
   110821 tgttgcggaa aatactgtag atggtataat tgcacctatg ttttatgcgt ttattggagg
   110881 agcaccactt gctatggcct ataaagctat aaatacattg gattcaacag ttggatataa
   110941 aaataaaaag tataaagata ttggttttgc atctgctaag attgatgata tagccaatta
   111001 tataccagct agaatatctg ttattcttat gacaattgga agtttctttt taagttatga
   111061 tcatagaaac tgttttaaaa tttctattag agatagaaag aatcataaaa gtccaaattg
   111121 tgctttttca gagggtgcag tatctggtgc tctagggata caactgggag gtaccaacgt
   111181 ttattttggg gaaaaggtat ataaaccaac aataggggat aagcttaggg agatagataa
   111241 agaagatata attaaaacta ataaaattat gtatgcatct tcatttgtat ctatgttggt
   111301 atttacacta atatatgtgt tatgtaggat gattttaatc aacttaatta tataaattat
   111361 tgtttgttaa cttaaggggg acatagatga aggatttagg gcatggagcc aatgtagatg
   111421 aaatggcaag attatatggg aaagacccaa aagaaataat tgattttagc tcaaatataa
   111481 atccaaatgt attaccaaat ttagaaagat acattttaaa aggattagaa gagtgtagga
   111541 attatccgga tataaattat acaaacttga gaaaaaatat atcaaaatat atagatataa
   111601 atccagactt tataatacct ggaaatggtg caacagaagt gatttatttg cttatgaaga
   111661 gtattaaaaa aagattggct ataataaatc cgactttttc agagtataga agaagtgcta
   111721 agttaaataa tttagatatt atagatttag agttagaaag taactttaaa cttaatatag
   111781 atataataaa agaaaatata gaaagatttg atagtttatt tatatgtaat cctaataatc
   111841 ctagtggtaa tgtccaagat ttaaaggaac ttgttaactt gctaggtaaa cataataagg
   111901 tactaataat tgatgaaact ttcatggaat ttgttgaaga tgaaagtaaa catagtttgg
   111961 ttaaatatat agaatcaaat aaaaatatat tcataattaa agctgtgact aaattttttg
   112021 gtatgcctgg tttaagatta ggatatggtc ttacaagtaa tgctgaaatt atgaacaaaa
   112081 tttatgaata caaagagcca tggactatta attcttttgc agatatacta tccaatttta
   112141 tttttgaaga tagagagtat attaaagata gtaaagaata ttacataaaa gaaagaagct
   112201 atatgttaca agagttgaga aacataagaa atataaaggt ttatgataca gatgccaatt
   112261 ttattttgat aagaacatat aaaaaaacag ctaaagaatt gaaaaaagat ttgttcaagc
   112321 aaggaaacat attagttaga gatgcgtcta attttatagg attagatgat agttttataa
   112381 gagtggctat aaaatctcat aaagataaca aaattcttat agaaaatatg aaaaatttat
   112441 tgggtgatta aaatgaaatc ttatggaata tgcccagcat cttgtggaga atttgtacaa
   112501 ggtataattg atgatgaaga atatttatgt tcatatgcaa tagatatgta ttctaaagtg
   112561 tatattgaag aaaaattagt agatataaat ttagggaggt ataagtcaag gttagcaatt
   112621 gaaaaggtat ttgaaaaatt taatttacct aaaaaagata ctaaaaatat ttctttaaat
   112681 ataaatagta aaatacctgt tggaaaagga atggcaagtt caactgctga tataggagct
   112741 accataaagg caacattatc attgatagat aaagatttaa gtagtgaaga gatttctaaa
   112801 ttggccgcag aaatagaacc aacagattct atatttatag ataaaaacag tatatttaat
   112861 cctctaaatg ggactgttat aaaataccta ggaaatctga ccaatgctaa agttataata
   112921 cttgaaccaa acaaagtact agatactatg aaaattaggt tgaggcaaga ttatagtaga
   112981 ttaaaaacag aaaataaaga agtcataaaa aaatcatttg ctcttttaga agaaggattg
   113041 aaaaaaaata atttatctct agttggagag gcttgtactt taagtagctt agcaaatgag
   113101 aatatagaca aaaaagagta tttaaatgaa ataataaaaa tatcaaaaaa atatggagca
   113161 tatggagtaa atattgctca tagtggaact gtagtaggta ttttaattga taagttcatg
   113221 aatgataaaa atatgataga tgccttgtgt gaatctaaca taaattctgt ctatagtaaa
   113281 atttatacac taaacataat taatggagga attaaggggg aaatagaatg gaatacataa
   113341 agaatcctat gaaaatagaa gaaaaaagtt ttgaattgat acaagaaata atagacgaaa
   113401 ttagaccaga ttataaattt aagaataata tcgaagaaaa aataataaag agagctatac
   113461 atactactgc agattttgat tacttagata tattaaaaat atcagaagaa gcagttgcga
   113521 gtataataga tgcattaaga aataatgcaa gtatttatac tgatactaat atggctctta
   113581 gtggtataaa taaaagaaag ttagaatcat taggatgtaa gtataaatgt ttagtggcag
   113641 atgatgaaac tgtaaaatta gctaaagaaa gaggaattac tcgttctatg gcagcggtag
   113701 aaattgcagc taaagaagaa ggaagaaaaa tattcgtttt aggcaatgca ccaacagctt
   113761 tatataaagt tatggagatg aaatcagaag gtagattaga tttagatgca gttataggag
   113821 ttcctgtagg ttttgtagga gcagaagaat caaaagatga agttgaaaaa acagatatac
   113881 catatataat ttctaaaggt agaaaaggtg gcagtaattt agcagcagca atagtaaatg
   113941 ctatattata tactatgtag gtacatatat ggaagaatat gtatatatag atggtaaaaa
   114001 atatagaaga ggatatacta caggttcatg tgcaacaggt gcatctaagg ctgctgtata
   114061 tatgttgatt acaaaaaata gaatcaatac tataaatata gacactccta aaggaatacc
   114121 attatcacta aaagttgata atataaatat ttctgataca tttgtggaat gttcaataaa
   114181 aaaagatggt ggagatgata tagatgcaac tcataccatg gatatttatg caagagctga
   114241 aattgtatct aaaaatgata agaacaaaga gtatcttact ctaaaggata tagatggttt
   114301 aagtaacaat agtgaatccc aaagtgaact ctataagttt attcgggttt atggtggaat
   114361 tggtattggt gtggttacga aaaaagggct tagtgtagat gttggtaagc ctgctataaa
   114421 tccgactcct cttaaaatga taaatcatga aataagaaat cttataggag ataattttga
   114481 atctatactt ggaaataata aagttttaaa aataacaatc tttgctccac aaggagaagc
   114541 tgtagcaaaa aaaacattta atccaagatt aggtatagtt ggagggatat ctataatagg
   114601 tacaactggg attgttgaac ccatgagtga tgagggctgg aagaagtctc tatctataga
   114661 acttcaaatg aaaaaagaac aaggtttaga taaaataata ttggttccag gaaatcatgg
   114721 agaacagttt ataagagaaa aattaaattt agatattaag tatgtagtta ggacaagtaa
   114781 tttcataggt tatatgatta aagaagctca aagaatagga tataaaaaaa tcttaatggc
   114841 aggtcatata ggtaaattta taaaaatatc tgctggtatt tttaatactc atagtaaggt
   114901 ggcagatgct agaagtgaga tattagtggc aaacttagct ttaatgggag ctacatacga
   114961 atttttaaat aaaataaatc aatgcctaac tactgaagaa gctgtagaat taataaataa
   115021 tagtgagtat agacaagtgt ataatatatt gagtaataaa tgcagggaga gagtaaagca
   115081 atacttgaat gaagattcag atgatattga tgtagaagta atcatatttt caatggataa
   115141 gtctttatta ggtaaatcgg ataatacaga tgatttagtg gaggtcttta tatgataaac
   115201 ataataggtc taggacctgg taacttagac tatataacaa aaaaaggtga aaatttaatt
   115261 tccacttctg atgtattaat tggtggtaag agaaatttag agtctataaa aaactttgaa
   115321 ggagaaaaaa tagtcttaga ttctaattta agagaaatta tagagtacat aaataacaat
   115381 aaagaaaagc aaatatcaat aatagcatct ggtgacccat taatatatgg gataggtaga
   115441 tatatatcta aaaatattga taataagatg ctaaatatgg tttctgggat tagttcatta
   115501 cagtacatat tttcaaaaat atatgttgat atgaatgatg tatatattac aagtagccat
   115561 ggtaaagtac cagattttga ttacatatta tcacataaaa aagtatgtat ggttacagac
   115621 tctaagatag gaccaaaaca aataagtaga gaagtaatag atagaaatct aaataaaatt
   115681 attgttgtag gagaaaattt gtcatatgac aatgaaaaaa taacaatagc aaaaccagaa
   115741 gagataataa gaatggacaa ttttgatatg aatgttgtgg tgattttaga tgaggaatag
   115801 tgaatttgta acaggtaaag ttccaattac taaggaagaa gttagagcaa tatcaataag
   115861 caagttaaac ttagtaaatg ctaaaaactt catagacata ggagctggaa caggaagtgt
   115921 aagtgttgaa gctgcatata attatcctaa tttaaaggtt atatctatag agaaaaatga
   115981 tgatgctata gagcttatag agaaaaatat aaggaagttt aatttaaaaa atatagaagt
   116041 gataaaagga tatgctccaa ttaacctagg attaaatact aaagttgagt ctatttttct
   116101 tgggggaaca ggaaataatc tggaagaaat aatagaatgg tctaaaaaaa atttggttgt
   116161 aggaggaaga cttgtagcta attttataat aatagataca tttaatcaaa ctttaaaact
   116221 gcttaaaaag catggattta aagaaataga tgtatgtgtt ttgaatgttt ctaaactaga
   116281 gaaactagga aaaggtgaat actttaaacc actaaatcct atatatataa tatcttgtga
   116341 aaaaggggaa aatgatgatg aataaagtac attttgtagg agcaggacct ggagataaag
   116401 agttgataac attaaaagga tataaattat taagtgatgc agatgtagtg atatatgcag
   116461 gttcacttgt taatcctgaa cttttggaat actgtaaaga agattgccaa atacataata
   116521 gtgcacatat ggatttacaa gagataatag atgttatgag agaaggtata gaaaataata
   116581 agtctgttgt aagactgcaa acaggtgatt tttctatata tggttcaatt agagagcaag
   116641 tggaagattt gaataaattg aatatagatt atgactgtac tccaggagta agttcttttt
   116701 taggtgcagc atcatcttta ggagttgaat atacagttcc agaaatttct caaagtgtaa
   116761 ttataacaag aatggaaggt agaactccag ttccagaaaa agaatcaata caatcatatg
   116821 ctaagcatca aacatctatg gtaatatttt tatctgttca agaaattgag aaagttgtgt
   116881 ctagattgct ggaaggtgga tatccaaaag atactcctat agcagttata tacaaagcaa
   116941 cttgggcaga tgaaaaaata gttaaaggaa ctttaagtga tatagctgta aaagttaaag
   117001 aaaacaacat aaataagacc gctttaataa tggttggaag atttttagga gaagagtata
   117061 ataattcaaa gttatatgat aaggatttca aacatgaata tagagggtaa tattgctata
   117121 atctgtatca cagaaaatgg gaaaaaatta gctgtaaaga taaattctct agtaaataac
   117181 tgtgttatat atcaaataaa aaataaaaaa agcaatttaa atgttgaaga aacgtgtgta
   117241 catacagttc aaaagaaatt aagtgatttt gtaggagaaa tatttaataa atttgattac
   117301 atagtattta taatggctac aggtatagtt gtacgaagta tagcaccata cataataagt
   117361 aagtttagcg accctgcagt tttggtaatg gatgaaaaag gcgaaaacgt aataagttta
   117421 ttgagtgggc atattggagg ggcaaacagt ttgacacagt acataagtag tcttattgat
   117481 gctaatccag taataacaac agctactgat gtaaatcaaa aagcttcttt agatatgata
   117541 gcaaaaaaac taaatgcata tattgatact tttagagaaa gtgtaaaaga agtaaattct
   117601 atgctagtga acaataaatg tgttggatta tatattgatg aaggatatca agttgatact
   117661 agaggattta aacttctaga aagtaaggat gagttaagag atagtttttc aaatatatat
   117721 atagaaaaat tagttgtagt aagcaataaa ataaattgta ttgaagatat tgagaattta
   117781 aacttagata ataaatttga actttactat agtactgaaa aaataataaa agtaattcca
   117841 aaagatatag taataggaat tggatgtaga agaaacacag atagtgagtt gatgtataat
   117901 tcacttaaag agtttcttaa taaacaaaat atagatatta gagcaataaa tacagttgga
   117961 agtatagaac tgaaaaaaga cgaaaaagca ataattgaat tatcaaaaac actaaaagtg
   118021 ccatttaaga cattttcaat agatgaaata tctaaggttg ataatctttt tgataaatct
   118081 gattttgtta aaaaaaatgt gggcgtgtat tcagtagcag agcctgtagc atatcttttg
   118141 agcaaaggta atttgttagt tgaaaaacat aaatataaag gaataacttt ttcagtgggg
   118201 agaataaaaa tatgatatac gttgtaggaa ttggaccagg aagtaaagat acaatgactt
   118261 tagaagcaat aaaagcaata gaagattctg aggctattgt tggatataag acttatataa
   118321 aattaataga agaatttata caagataaag aaataatcca aaatggtatg aggcaagaag
   118381 ttgatagatg taaacaagca gtagcggaag caaaaaaagg aaaaaaagta gcagttgtaa
   118441 gcagtggtga tgctggaata tatgggatgg caggtcttat tttagaatta atatctaaag
   118501 aagatgaaga tataaaagta aaggtaattc caggtgttac tgcaagtatt ggtgcagcag
   118561 ctatactcgg agctcctatt atgcatgatt tttgtcacat aagcttgagt gatttaatga
   118621 ctccgtggga agtaattgaa aaacgattga gattagctgc tgaagctgat tttgtaattt
   118681 gtttgtataa tccaagaagt aagggaagaa gtgaacatct aagcaaagca tttaaaataa
   118741 tgggtgagtt taaagatgga gatacacctg ttggagtggt aaaagatgta ggtagagaaa
   118801 aagaagaaaa gtttgtatgt acttttgaaa atatggactt tgaaaaagtc gatatgacta
   118861 caatggttat aataggaaat aaatctactt atattgatgg tgaattgatg ataactccaa
   118921 gaggttatac agtatgattt taattttagg aggaacttca gatagtttat ctatatgtga
   118981 taagataaat gaactgaaaa accaaccata tattctttca gtgactacag attatggaag
   119041 ggaacttgca caacaacatg caaataatgt aattttaggt aagcttggaa aagaagatat
   119101 gttaaaattt ataaaggata ataatatagt aaaaataata gatgccactc atccttatgc
   119161 agttgaagta tccaaaaatg ctattacttg tgcaaaatta tcgaatatag attatataag
   119221 atttgagaga aaatctttaa ttgatgaaat agattatgat aataaattta tagtgaatac
   119281 aatcgaagaa gcctgtgaaa tagcaaataa aatagggaca aatatattca taggaacagg
   119341 aagtaaaaat ttgaacaggt ttatagagga aataagtgat aaaaatttag ttgtgagagt
   119401 tcttccaact agtgaagtca tattaagttg tgaaaaatta ggtttaagtg cagacaatat
   119461 aatagctatg aaaggtccgt ttagccagta tataaatgaa gaaacatata aacattacaa
   119521 tatagattta gttataacaa aagaaagtgg aatagctggt ggattcttag agaaggtaaa
   119581 tgcatgtaaa actttcaata taccagtaat aattataaaa agagaatcaa ttaactacga
   119641 aaatgtattt aatgacataa ataaaattaa agacatactt gtgtaatttt taggaggaga
   119701 atatatgaag aaagccttat tagtagtgag ttttggtaca agctatcatg aaacgagaga
   119761 aaagacaata gatatatgtg aagataaaat aaaaaattca ttaaaaagtt atgatttttt
   119821 tagagcatat acatcaaata tgataataaa taaaataaaa aaaagagatg gaatagaaat
   119881 agataatcca atacaagctt tagataaaat atatgagcaa ggatatgatg aagtaataat
   119941 ccaaacactt catattatat gtggcgaaga atttaacaaa ttaaaagagc aagtagatgg
   120001 ctattcttcc aaattcaaaa agattgtatt aggaagacct ttacttacac atattgaaga
   120061 ttatcaggaa gctgtagagg caattaagca tcaaattcct catatggaaa gtgatgaagc
   120121 agtagtattt atgggacacg gaacacttca tgaatcacat tcagcgtatc ctgcattaga
   120181 atacatgctt agagacaatg gagtaaatgc ttatgttggt acagttgaag ggtatccaga
   120241 aatagaacat gtaataagaa gactgaaaga aggaaatata aaaactgtaa atttgatgcc
   120301 atttatgtta gttgcaggag accatgctat aaatgatatg gcaggagatg aagaagattc
   120361 ttggaaaaca atattagaag aagatggatt taatgtaaga gttcatttaa aagggctagg
   120421 agaaaattcc tatatacaag ataaatttgt tcgtcatgct ttaaaatgtg cagagaatgc
   120481 taagttttat ggtataggag cgggtccggg agatggttct ttacttacaa taaaagcagt
   120541 taatacttta aagaaattgg atattctata cactccagaa tcaaaaaagg gtggagatag
   120601 tctagcttta tcaatagtta gtgaatactt acctgaatca gtggaaataa aatctaggca
   120661 ttttccaatg agttttgatg ggaatgaaaa aagtttagct tgggataatg tggcacaaga
   120721 aattatatca gatgttaatg atggtaaaaa tgtaggattt gttacattag gagaccctat
   120781 gatttatagc acgtatgtat atataatgaa aagaatatta ggagaggtag aagtcgaaac
   120841 tataccagga atttcatcat tttcgaatat agcatctaat caaaattttc cattagttat
   120901 ggacaaagaa gcattagtgg taattccttg tactatggaa gatgataaaa tagagtatgc
   120961 tttgcaaaat tataactcaa tagtattgat gaaggtatat aaaaatttca aagaaataat
   121021 aaaaaaatta gaaaagtatg atttaataga acatgctatt ttagtaagta attcttccca
   121081 agaaagcgaa aatgtattta cagatttaaa agaagcgcac cttgaagata agatatctta
   121141 tttttctact atactagtaa ataaagataa taaaataaaa taggtgaaag caatatttaa
   121201 aggagattta atatgaatat agtggttgga actaggggta gtaatctagc acttatacaa
   121261 acagaatggg taataaatga attgaaaaag aagtatccag aaattagttt tgagattaaa
   121321 atcataaaga caaaaggaga tttaattcaa aatgtatcac ttgataaaat aggtgataaa
   121381 ggattgtttg ttaaagaaat agagcaacaa ttattgtatg gaaaaataga catagcagta
   121441 catagtatga aagatatgcc ttcttattta gcagatggat taaaatttgc tcatacgcct
   121501 aagagagaag acccaagaga tgttctaata cttagggaag gatataaaaa tttagatgat
   121561 ttgcctcgtg gagcagtaat aggaacagga agcaaaagaa gaaagtttca attattgaaa
   121621 caaagacctg atttaaatat agtacaagtt agaggtaatg tggaaactag gataagaaaa
   121681 ataaaagatg aaaatatgca tggtattgtg ttggcagcat caggaataat tagagctaat
   121741 ttacaagata aaataagttc ttatttacct gttgatgttg ttattccagc acctgcccaa
   121801 ggagccttag ctatagaaat tagaagtaac gatagtaaga tagagggaat agtaaattca
   121861 ctgaaagatg agaatacaga aattcaaata ttggctgaaa gaggtttttt agatggtgta
   121921 aatggaagtt gtcatattcc aatggcagct tattgtgaaa taatccaaga taaaatacat
   121981 cttactggat tatatggaga ttcagaagga aaaaaagtag taatcaagtc tatagatgga
   122041 gatatttctt ctcctagaga gttaggttta aagttagcta aacttgtgtt gaaggagtat
   122101 gagaattatg aagggtaagg tttacttagt aggggcagga ccaggtgatt ataaactcat
   122161 gactttaaag ggtttagagt gtataagaaa atcggatgta atagtatatg atagattagc
   122221 aaatagtaat tatttaagag aagctaagcc tgattgtgaa tttatatatg taggaaaggc
   122281 atcgagtaat catattttgc cacaagaaga tataaataga attatagcag aaaaagctaa
   122341 agaaggaaaa attgtaactc gtcttaaagg gggagaccct tatgtatttg gtagaggggg
   122401 agaagaagca gagactttag tagatgaagg tatagaattt gaagtagttc ctggtataac
   122461 ttctgcaata ggaggtttat gttatgcggg tattcctata acacatagag accatgcttc
   122521 gtcatttcat gtaataacag gtcatctgaa gggtgatgat agtggagaat taaactggaa
   122581 tgctcttgca aataataaag gtactcttgt gtttttgatg ggaatctcaa acctaaataa
   122641 aataagtgaa aacttaatga aagaaggtaa agataagaat acacctgttg ctcttataag
   122701 ttgggcaact agatataatc aaagagttgt tacatctaca cttgaaaatg tttatgaaac
   122761 tgctattaaa gaagaggtaa aaccgccaac tcttatagta gttggaagtg ttgtagaact
   122821 tagagaaaag ttgaatttct ttgaaagtaa acctttattt ggaaaaagta ttgctgtaac
   122881 taggtctaga aatcagaata gtgtattagt agaaaaaata atggatttag gtggaaatcc
   122941 aatagaaata cctactatta aggtagaaaa aatacaaaac aatattaaat tagaaaatga
   123001 aataaaaaat ataaataagt ataattatct aatactaact agtaaaaatg ccgtagaaat
   123061 tttctttgaa aagatatatg aaatgaattt tgatttaaga atactatcta atttaaaggt
   123121 atgtgctata ggttctgcta catcaaacga gttgaaaaag agaggaatta tagcagatat
   123181 agtacctaaa aaatttgtgg cagaatcact gtatgaagag ttaatgccta ttttaaataa
   123241 tagtgataat atacttatac ctagagcaga aaatgctaga gattatctgg ttgacaaact
   123301 taaggccatt tgtgaagtaa cagaagtaca tatatataga acagtaatag atgaggataa
   123361 aaaagatgag atattagata ttttaaattc taataatata gattatataa catttactag
   123421 ttcctcaaca gtaaataatc tcgttgaaat tataggaaaa gaaaatatta aaatattaga
   123481 aaaaataaaa acattatcta tagggcctat aactacagat acaataaata gtttgggaat
   123541 aaaattgtac aaagaatctg ctacatctac tatagattcg ttagtagatt taattataaa
   123601 aaattaattt tggaggcttg aatatgttaa gaagaccaag aagactaaga gcaagtaaag
   123661 caatcagaaa tttggtaaga gaaactaaag tgaatgtaga agatttaata tatccactgt
   123721 ttgtagtaga gggggaaaat ataaaagaag aaattaaatc tttaccagat gtctatcatt
   123781 tatcaataga catgttagaa gaagaaataa aagaaataag tgagttggga attgaacata
   123841 taatgttatt tggtgttcca gatgaaaaag atgagtgtgc ttcacaagct tttaatgata
   123901 atggaatagt tcaaaaggca attagaaagg taaaagaaat aaatgcagat atgaatgtaa
   123961 taacagatgt ctgtatgtgc caatatacaa gtcatggaca ttgtgggatt ttaactgata
   124021 ctggttatgt tgataatgat aaatcattag agtatctagc taaaatatct gtaagccatg
   124081 ctaaagcagg agccaatatg gttgctccat ctgatatgat ggatggaaga atacaagcaa
   124141 taagagaagc tttagatgaa aatggattag aaaatgttgg aattatggct tatagtgtta
   124201 agtatgcttc aaattattat ggacctttta gagaagcagc tcactcagca ccatcttttg
   124261 gagatagaaa aacatatcaa atggatccag caaatagtaa tgaagctata atagagactg
   124321 aacttgatat attagaaggt gcagatatac ttatggttaa acctgcatta tcatatttag
   124381 atattataag aagggttaaa gataattttg atttacctct agcagcttat aatgtaagtg
   124441 gagaatactc tatgcttaag tttgctgtga aggaaggttt attaaatgaa gatgtaattc
   124501 ttgaatcaat tacatctata aaaagagcag gagcagatat aataataact tattttgcta
   124561 aggatattgc caagttatta aaaaataaat tttagctaga aaaagaattt taaaaagtat
   124621 aggaattgta attagatatt atataatatt tttatttaat ttgttgaata agaatagata
   124681 ctaatttata atttctatgc tttatttgta taatcaaata aagtttggag gtataaaatg
   124741 ctatatccta ttaatttaaa attagatgac ttagatgtag ttataattgg tggaggagaa
   124801 gttgcatata gaaaatgtaa aaacttttta aatttcaata aaagagttac tatagtttct
   124861 aaacaactgt taaataagtt ttatgatttg aaagaataca taaatataat agaagatgat
   124921 tataaagaag tgtatataga aaaagcctct atagtaattg ctgcaacaaa caataaagta
   124981 ttgaatatgg aaataggatt atattgtaga gcggaacata aattagtaaa tgttgttgat
   125041 aatgtagaaa tatcaaattt tactgttcca tcatatataa aaagaggcga tttacttata
   125101 agtgtatcta ctggaggcaa gagtccttca ctgtcgtcaa agataaaaaa agagattgaa
   125161 gagatatata ctgaagatta tgaggagtat ttaaatgtat taggagatat aagaaaaaag
   125221 gttataaaaa aatacgaaga taaaagtaaa agaaaagatg tgcttaacat gttgataaca
   125281 cttgacttag aagaacttaa gaagtttgat atataaaaaa taaaataaaa aatgttaaaa
   125341 aagtgttgac g
//
LOCUS       DAMJVM010000002       108149 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.002, whole genome shotgun sequence.
ACCESSION   DAMJVM010000002 DAMJVM010000000
VERSION     DAMJVM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 108149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 108149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..108149
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.002"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(208..621)
                     /locus_tag="PC523_000128"
     CDS             complement(208..621)
                     /locus_tag="PC523_000128"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDF2473702.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRV"
     gene            complement(662..1792)
                     /gene="rodA"
                     /locus_tag="PC523_000129"
     CDS             complement(662..1792)
                     /gene="rodA"
                     /locus_tag="PC523_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDF2473703.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNFLGRYYKALYIISLVLLAIVLLPGIGS
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVIVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQENFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            complement(1876..2160)
                     /gene="minE"
                     /locus_tag="PC523_000130"
     CDS             complement(1876..2160)
                     /gene="minE"
                     /locus_tag="PC523_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDF2473704.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            complement(2178..2975)
                     /gene="minD"
                     /locus_tag="PC523_000131"
     CDS             complement(2178..2975)
                     /gene="minD"
                     /locus_tag="PC523_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDF2473705.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            complement(2999..3682)
                     /locus_tag="PC523_000132"
     CDS             complement(2999..3682)
                     /locus_tag="PC523_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDF2473706.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKVEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            complement(3780..6758)
                     /locus_tag="PC523_000133"
     CDS             complement(3780..6758)
                     /locus_tag="PC523_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDF2473707.1"
                     /translation="MEENKQVDRLKIIKIVMSVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKNLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLN
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKMNVKEDRIEDV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            complement(6770..7258)
                     /gene="mreD"
                     /locus_tag="PC523_000134"
     CDS             complement(6770..7258)
                     /gene="mreD"
                     /locus_tag="PC523_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDF2473708.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGANALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFIKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            complement(7273..8166)
                     /gene="mreC"
                     /locus_tag="PC523_000135"
     CDS             complement(7273..8166)
                     /gene="mreC"
                     /locus_tag="PC523_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDF2473709.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDIANFKDNSKKVKKLEEENEKLKKDMIS
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISTSVVSKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            complement(8176..9186)
                     /locus_tag="PC523_000136"
     CDS             complement(8176..9186)
                     /locus_tag="PC523_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDF2473710.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     VNMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            complement(9307..9966)
                     /gene="radC"
                     /locus_tag="PC523_000137"
     CDS             complement(9307..9966)
                     /gene="radC"
                     /locus_tag="PC523_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDF2473711.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGDKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKATQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSVGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            complement(10011..10595)
                     /gene="maf"
                     /locus_tag="PC523_000138"
     CDS             complement(10011..10595)
                     /gene="maf"
                     /locus_tag="PC523_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDF2473712.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLSDLLKKYFSINLFYGV"
     gene            complement(10815..11786)
                     /locus_tag="PC523_000139"
     CDS             complement(10815..11786)
                     /locus_tag="PC523_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HDF2473713.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     VAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            complement(11802..12377)
                     /gene="rsxA"
                     /locus_tag="PC523_000140"
     CDS             complement(11802..12377)
                     /gene="rsxA"
                     /locus_tag="PC523_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDF2473714.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            complement(12392..12985)
                     /locus_tag="PC523_000141"
     CDS             complement(12392..12985)
                     /locus_tag="PC523_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HDF2473715.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            complement(12998..13567)
                     /locus_tag="PC523_000142"
     CDS             complement(12998..13567)
                     /locus_tag="PC523_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HDF2473716.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMDKSTYKDLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            complement(13567..14544)
                     /locus_tag="PC523_000143"
     CDS             complement(13567..14544)
                     /locus_tag="PC523_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HDF2473717.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFAKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            complement(14567..15904)
                     /gene="rsxC"
                     /locus_tag="PC523_000144"
     CDS             complement(14567..15904)
                     /gene="rsxC"
                     /locus_tag="PC523_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDF2473718.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKTIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTVTGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDVQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            complement(16120..17064)
                     /locus_tag="PC523_000145"
     CDS             complement(16120..17064)
                     /locus_tag="PC523_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473719.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKNGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIDSISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            complement(17175..18449)
                     /locus_tag="PC523_000146"
     CDS             complement(17175..18449)
                     /locus_tag="PC523_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2473720.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSNPNNSTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGEVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGANNSSNSNSTNNSDKKPSSEESIEGKNGKVTSTVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKNNGWYKVKLSNGTTGWVSGSYISETNEGTKENSNSSSN
                     QNSQSNNNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            complement(18581..18952)
                     /locus_tag="PC523_000147"
     CDS             complement(18581..18952)
                     /locus_tag="PC523_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2473721.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            complement(19179..19778)
                     /locus_tag="PC523_000148"
     CDS             complement(19179..19778)
                     /locus_tag="PC523_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473722.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            complement(19979..20191)
                     /locus_tag="PC523_000149"
     CDS             complement(19979..20191)
                     /locus_tag="PC523_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDF2473723.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDSVSIEKLKEVIAELGFELKGIE"
     gene            complement(20264..21979)
                     /locus_tag="PC523_000150"
     CDS             complement(20264..21979)
                     /locus_tag="PC523_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2473724.1"
                     /translation="MKRIKVLTVVLAITLMVVGCSNSKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKDVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQSHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKALECLAKVEYANVNPVNQNDNKIKNQKKPAKKNLKKLTPEEEAKAKKAEE
                     DSIKKEAEEKQNREKEEVKKKLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKDYDLALVGWKLSSIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSSSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(21979..22533)
                     /locus_tag="PC523_000151"
     CDS             complement(21979..22533)
                     /locus_tag="PC523_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDF2473725.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEQYNKIYGKKGKN"
     gene            complement(22526..23128)
                     /locus_tag="PC523_000152"
     CDS             complement(22526..23128)
                     /locus_tag="PC523_000152"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDF2473726.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNISIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKKLNNLTHPRIREKIISEIEELR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALNRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(23137..25785)
                     /gene="polA"
                     /locus_tag="PC523_000153"
     CDS             complement(23137..25785)
                     /gene="polA"
                     /locus_tag="PC523_000153"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDF2473727.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFKINR
                     LEIDGYEADDIIGTVSKKAEDDGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLLENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLESMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSNDTEQVEEKIEILKLKDIEKFIDEVNEKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITISEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNIDVHLDVDLNVGSSWYETK"
     gene            26104..26186
                     /locus_tag="PC523_000154"
     tRNA            26104..26186
                     /locus_tag="PC523_000154"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26138..26140,aa:Leu,seq:caa)
     gene            26877..28286
                     /locus_tag="PC523_000155"
     CDS             26877..28286
                     /locus_tag="PC523_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HDF2473728.1"
                     /translation="MIFSSLIFLFLFFPVTLICYYLANPKYKNVMLLLASIAFYTWGE
                     PKYIFLMILSIIVNYIFGIKVSGSKNKKMWVVLSVIYNISILAVFKYSNFFIDNINFL
                     FNTDITIPEVKLPLGISFFTFQIMSYVIDVYRNDAKVQRKLANLALYISLFPQLVAGP
                     IVRYQTVADSIEYREHNWDKFTQGVNRFVVGLGKKVILANQLGVIADGVFNKSAGDLS
                     RFEVWLGIVCYTLQIFYDFSGYSDMAIGLGKMFGFEFLENFNYPYISQTVSEFWRRWH
                     ISLGTWFKDYVYFPLGGNRVSKAKLYRNLFVVWFLTGMWHGTSWTFIILGLYYGILIS
                     VEKAFLEKFLYKMPRIVRHIYLLLIVMIGWVFFRVDDLPAAIKFIGIMFGAGNYPVIS
                     NAFNVFIIEYWYVIVGAILLSTPIVKVLKSLLIKKNKNTLNSTLYQVTNMVFIVSCMF
                     IVIIMLSSSTYNPFLYFRF"
     gene            28302..29495
                     /locus_tag="PC523_000156"
     CDS             28302..29495
                     /locus_tag="PC523_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473729.1"
                     /translation="MSKKKPRIVAISFLIIVFGFFILNFTLPKKKLSITENRNLAKFP
                     TVENLKKGDFIAKFEEYFNDHFAFRDRMISINTKAQASLNKTKVGNYYLGKDNWILGM
                     FPEILDQKQLSRYEKSINYLSNLSKDMGKNVYFTMMPHKTNMLKHLYPKYVDNMDNID
                     INLKNFKNRLNPDLIKYIDMDEYFLSNYSDKERENLYFKTDHHWTGYGAFEGFKMMAS
                     NMDLGLSKQDLDKHFSSYKKSIITDKKFIGSYNQNIDMLIKENEYVVYMTKDNQDYSF
                     DINGEEKEEKEVYATERDNKEWDYGGAYIRGAKTNILTIKNPKALVNKKILIFRDSYQ
                     APTTLLFADMFKEVEIADPRNINSIYKTYKEIIEESNADIVMFMYNSSGFDSMIDSMI
                     DKGIK"
     gene            29612..29992
                     /locus_tag="PC523_000157"
     CDS             29612..29992
                     /locus_tag="PC523_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473730.1"
                     /translation="MSKLNRKKDFYFVSLPHKTNMLKNLYPSGIKGKNNIDINKENLE
                     KQLKDINFLDIDDYFYKSFTEKERENFFLKTDHHGNSLGAFEEYKFILDKLDLSLSKS
                     EIDSHLKRYSTRYVNDCECSFIYV"
     gene            complement(30512..30955)
                     /locus_tag="PC523_000158"
     CDS             complement(30512..30955)
                     /locus_tag="PC523_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473731.1"
                     /translation="MKKNMLYLGIVYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERALDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
     gene            complement(30958..31155)
                     /locus_tag="PC523_000159"
     CDS             complement(30958..31155)
                     /locus_tag="PC523_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2473732.1"
                     /translation="MKNRLEEIRKERGIKQEELASVLQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDRSIEEIFIYEEE"
     gene            complement(31347..32213)
                     /locus_tag="PC523_000160"
     CDS             complement(31347..32213)
                     /locus_tag="PC523_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2473733.1"
                     /translation="MEWINKLNQAITYIEENLESGIDYAEAAKIACCSTFHFQRMFSY
                     IAEVPVSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNVEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPILEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYSNLPDIELYPNGDINSPDY
                     TTEVWIPIQKPS"
     gene            complement(32438..33307)
                     /locus_tag="PC523_000161"
     CDS             complement(32438..33307)
                     /locus_tag="PC523_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2473734.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDIRIMNRSEVFNNLEKVAGYKGNMIDSPSYIIILSDVKDNYIENSGYIGENIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGIDSDKEVTGIIAIGYGENVNNAKVLNATKTGQNYSK
                     SDMQVVTDKGSSRLGVEDVVYMNEWGNVASIEVLEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVILAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFELKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(33813..34034)
                     /locus_tag="PC523_000162"
     CDS             complement(33813..34034)
                     /locus_tag="PC523_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473735.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEEHEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(34535..35515)
                     /locus_tag="PC523_000163"
     CDS             complement(34535..35515)
                     /locus_tag="PC523_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473736.1"
                     /translation="MGDTSRFYESVRNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDILSWKQIVVLAECDGKNTTDDIINILSNRYRNIPNMREKVMEFFMFYENV
                     YLTFEDNIQTVSSVFEITGNKSYITPLYFTLEIDADNNTNNYFSEITSLLKCMYEKGC
                     RFIEVIGEDILKNKGMRDVFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSRNDDTRIKLKEDIKITSLIKRGHTVVRRDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSNGNVKSYSFQKDKRVYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(35679..36551)
                     /locus_tag="PC523_000164"
     CDS             complement(35679..36551)
                     /locus_tag="PC523_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2473737.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPVEFDN
                     FKIVQVSDLHNKVFDKNNKVLMDKIKSLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINLENESVRIYKGNSHLNLYG
                     LIVPLECYPYFFANNKKLELSDNFVESNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRMPFVGGVLSPNREFFPKYDWGRYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKSKK"
     gene            complement(36715..37266)
                     /locus_tag="PC523_000165"
     CDS             complement(36715..37266)
                     /locus_tag="PC523_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2473738.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQQGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(37286..38188)
                     /locus_tag="PC523_000166"
     CDS             complement(37286..38188)
                     /locus_tag="PC523_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDF2473739.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCISCNRCVDVCPQNISSLFDRTQINNKCDLCGECIKACLKDAICIMT
                     EEMSVEEIVKEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYNKGINIAIET
                     CGYFDWNKVNDVFDKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPEGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(38203..40575)
                     /locus_tag="PC523_000167"
     CDS             complement(38203..40575)
                     /locus_tag="PC523_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HDF2473740.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDERTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKVLVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISISENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSEQETDKKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDNAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLNQLAYLVKNSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMVKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(40796..41500)
                     /locus_tag="PC523_000168"
     CDS             complement(40796..41500)
                     /locus_tag="PC523_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473741.1"
                     /translation="MGGLCMKVKRNINKVAIVVALNLSLALMVGCKSPVSDKNLSSSK
                     QISKDSLKESVEDKKDESIELFNNYSQKMNDVKGREEVKKDLSQRFSKAIIDSGYSIT
                     NSKSDGSFVVSINSVEKPPEENIRSLFYSVSEDDKEGKVTIDILCAKEYPEDEKLSES
                     DKYIKFIYNLFKSLTNTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKIKQST
                     KVLKLSLLYKFDISNR"
     gene            complement(41726..43105)
                     /locus_tag="PC523_000169"
     CDS             complement(41726..43105)
                     /locus_tag="PC523_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2473742.1"
                     /translation="MLNFMTELLVVLSIFIQFSIMNGILIYKKDSKKNEKIVFYIVLF
                     LIYIIVQISRLFNGGNYFSNINYLFYILAFIAWGGYYNYFYKIGYIKYSVFFYLFSVY
                     YECLDAVIYRILFFAITGNVISSKESLLIPHLESIKIQIYSNLIIILIYLIFSFFRKS
                     IKLISQDKRNYVYLLFALLANTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGA
                     SSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIY
                     CIDKLRNNSKEIDEYVNSLKDEIKAFKYIYNTGNMILDIIINEKSEICLKKGIKFICS
                     INFSKVNFVKPIDVSSIFSNVLDNAIEACDKILDENINKYIRIKGTITKSFFVLKCEN
                     SKLNKITFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELSFENSMDKFILNIYIPL
                     DQDTDSWSD"
     gene            complement(43099..43812)
                     /gene="rgbR"
                     /locus_tag="PC523_000170"
     CDS             complement(43099..43812)
                     /gene="rgbR"
                     /locus_tag="PC523_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HDF2473743.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILEDINIEYEIQKYLSGEDLL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFITSLIDYVQEGYEVRAYRY
                     LLKPIELEDLKKHVLTCIKDIEINKKSHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLSKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTVILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
     gene            complement(44059..44619)
                     /locus_tag="PC523_000171"
     CDS             complement(44059..44619)
                     /locus_tag="PC523_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HDF2473744.1"
                     /translation="MSVMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYRINKFGGI
                     FYFHKESPRKIKYTVDYYYDAENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKKNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGCGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            complement(44715..45518)
                     /gene="zupT"
                     /locus_tag="PC523_000172"
     CDS             complement(44715..45518)
                     /gene="zupT"
                     /locus_tag="PC523_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDF2473745.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGGWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGVFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            complement(45669..46832)
                     /locus_tag="PC523_000173"
     CDS             complement(45669..46832)
                     /locus_tag="PC523_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2473746.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     MGIPYVSAGGTGVIATIKGANSGKTVALRGDMDALQVVECTEVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAVLEGTIRLFNPELRKKIPGILERIAKS
                     TAGAYRAEAELEYGYLTPAVINDKECSKIATEAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            complement(46892..48079)
                     /locus_tag="PC523_000174"
     CDS             complement(46892..48079)
                     /locus_tag="PC523_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473747.1"
                     /translation="MEAITAFCLVLISLTIGDVVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTAEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVMCLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIAGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            complement(48442..50142)
                     /locus_tag="PC523_000175"
     CDS             complement(48442..50142)
                     /locus_tag="PC523_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2473748.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIRDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPEGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            complement(50382..50657)
                     /locus_tag="PC523_000176"
     CDS             complement(50382..50657)
                     /locus_tag="PC523_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2473749.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEIEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            complement(50776..51327)
                     /gene="mobB"
                     /locus_tag="PC523_000177"
     CDS             complement(50776..51327)
                     /gene="mobB"
                     /locus_tag="PC523_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDF2473750.1"
                     /translation="MKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGYRVGVIKH
                     TCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKDKSPLYKMIQLYEDMDLI
                     IIEGYKNYRFKRLEVTRKDKYEDIFSNKSDLVGIVSDIKYNLNIKQFDLNDYKNISKY
                     IESCIKNDILSISNVEIKNILKE"
     gene            complement(51332..52513)
                     /locus_tag="PC523_000178"
     CDS             complement(51332..52513)
                     /locus_tag="PC523_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDF2473751.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKRVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGEVFNRVLNKGEAVKIMTGGKIPRGANC
                     VIKKEDVTVIGDMITLNVKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVRDMLQNADFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVATLGEKLLFGLSGNPHAAIIVFDNVVVPVIEYLTYKKGIHYIKAIFKGEFNKLSN
                     KDRFVNGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDIVI
                     R"
     gene            complement(52725..53459)
                     /locus_tag="PC523_000179"
     CDS             complement(52725..53459)
                     /locus_tag="PC523_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473752.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINGNKVVNVETNSFSSQGIAVVDAESSIVIEKSDHEKSAVSLENFR
                     RIDLGEYVGVEESRITEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            53822..54727
                     /locus_tag="PC523_000180"
     CDS             53822..54727
                     /locus_tag="PC523_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2473753.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVNK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            complement(54887..55729)
                     /locus_tag="PC523_000181"
     CDS             complement(54887..55729)
                     /locus_tag="PC523_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2473754.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(55713..56471)
                     /locus_tag="PC523_000182"
     CDS             complement(55713..56471)
                     /locus_tag="PC523_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2473755.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHLADRYAEEGNIEGVERIQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            complement(56488..56958)
                     /locus_tag="PC523_000183"
     CDS             complement(56488..56958)
                     /locus_tag="PC523_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2473756.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDELLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDINMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            complement(56958..57701)
                     /locus_tag="PC523_000184"
     CDS             complement(56958..57701)
                     /locus_tag="PC523_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HDF2473757.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEIAIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENNIEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLELGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            complement(57705..58118)
                     /locus_tag="PC523_000185"
     CDS             complement(57705..58118)
                     /locus_tag="PC523_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2473758.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVKLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            complement(58143..58895)
                     /locus_tag="PC523_000186"
     CDS             complement(58143..58895)
                     /locus_tag="PC523_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2473759.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            complement(59135..60529)
                     /locus_tag="PC523_000187"
     CDS             complement(59135..60529)
                     /locus_tag="PC523_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2473760.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSRNYMEFLSKSKTERLSV
                     KEIIKLAKENGYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAVPLALHGIVILNDGTKVEIS
                     IGEEDNDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNIPLDDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            complement(60549..60758)
                     /locus_tag="PC523_000188"
     CDS             complement(60549..60758)
                     /locus_tag="PC523_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDF2473761.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            complement(60786..62231)
                     /locus_tag="PC523_000189"
     CDS             complement(60786..62231)
                     /locus_tag="PC523_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2473762.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESMDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINEKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            62341..63135
                     /locus_tag="PC523_000190"
     CDS             62341..63135
                     /locus_tag="PC523_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDF2473763.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQNKYKDKISIKKGIEFGVQTQLIDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLRQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            63185..64507
                     /locus_tag="PC523_000191"
     CDS             63185..64507
                     /locus_tag="PC523_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2473764.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALTISIV
                     ASFNSSFLSIKFLKDAKLNLFILAVCPAIVIITISNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGVISG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKAIH"
     gene            complement(64607..65839)
                     /gene="cdeC"
                     /locus_tag="PC523_000192"
     CDS             complement(64607..65839)
                     /gene="cdeC"
                     /locus_tag="PC523_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HDF2473765.1"
                     /translation="MQDYKKNKRRMTNQPMPTMNEEEVYTEEINSEDMRGFKKSHHHN
                     ECNTDNKCDCHDDCKPCNPCKPNPCNPCKPNPCNPCKPNPCDDNCGCHDHCKCDCEPC
                     EMDSDECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGE
                     PLTFETEVVEVFGSVPSAGKASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGR
                     NCETTFEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALA
                     FPAVRAGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILK
                     LECNECCEPFYELIIPNDIDLVLCLQNTVSTLISEQIVVLASPNPIQPRLVDTFSKVC
                     DFSQCGPNHESGKPSCHR"
     gene            complement(66024..66204)
                     /gene="ssrS"
                     /locus_tag="PC523_000193"
     ncRNA           complement(66024..66204)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PC523_000193"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(66319..67230)
                     /locus_tag="PC523_000194"
     CDS             complement(66319..67230)
                     /locus_tag="PC523_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473766.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHSNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSKDERKILIKIESVL
                     DLVRGIKKLNNIDMVDIEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNEDEDNN
                     EGLNNEDEELDYEDKELDYDDEELTDEDEMEDNEDELVEDNKTDYENIENDEDIVYDE
                     ELYNINEQESEYNYNAKNTKTRTKKKR"
     gene            67442..67840
                     /locus_tag="PC523_000195"
     CDS             67442..67840
                     /locus_tag="PC523_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473767.1"
                     /translation="MKKLDAESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDTIMEKISDAETQRDTKPAKTKKTKPTKGKS
                     NSPAPKNTPTGQQKKSKGLFKKTKKSNPHE"
     gene            complement(67962..68978)
                     /gene="ccpA"
                     /locus_tag="PC523_000196"
     CDS             complement(67962..68978)
                     /gene="ccpA"
                     /locus_tag="PC523_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HDF2473768.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVSGF
                     NDVKIAKMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVI
                     ERKK"
     gene            complement(69231..69431)
                     /locus_tag="PC523_000197"
     CDS             complement(69231..69431)
                     /locus_tag="PC523_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473769.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSSGGFFDGEVGCGC"
     gene            complement(69465..69644)
                     /locus_tag="PC523_000198"
     CDS             complement(69465..69644)
                     /locus_tag="PC523_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473770.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            complement(69826..70032)
                     /locus_tag="PC523_000199"
     CDS             complement(69826..70032)
                     /locus_tag="PC523_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473771.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            complement(70080..70505)
                     /locus_tag="PC523_000200"
     CDS             complement(70080..70505)
                     /locus_tag="PC523_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473772.1"
                     /translation="MKYVGLLLTSIGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            70677..70904
                     /gene="acpP"
                     /locus_tag="PC523_000201"
     CDS             70677..70904
                     /gene="acpP"
                     /locus_tag="PC523_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDF2473773.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            complement(71045..72049)
                     /locus_tag="PC523_000202"
     CDS             complement(71045..72049)
                     /locus_tag="PC523_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDF2473774.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVPNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(72063..72965)
                     /locus_tag="PC523_000203"
     CDS             complement(72063..72965)
                     /locus_tag="PC523_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2473775.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKINEEKANFEPQDLNLQIIYDDFDIIMVNKLPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGCVDEELINRQALHAYRLEFEQP
                     RTKEKLKFKADIPEDIKELISKLR"
     gene            complement(73162..74337)
                     /locus_tag="PC523_000204"
     CDS             complement(73162..74337)
                     /locus_tag="PC523_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDF2473776.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTSLIVKR"
     gene            complement(74425..75270)
                     /locus_tag="PC523_000205"
     CDS             complement(74425..75270)
                     /locus_tag="PC523_000205"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HDF2473777.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            complement(75368..76165)
                     /locus_tag="PC523_000206"
     CDS             complement(75368..76165)
                     /locus_tag="PC523_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDF2473778.1"
                     /translation="MSTSDVKVYENVAVEVNGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPEILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            complement(76184..77194)
                     /locus_tag="PC523_000207"
     CDS             complement(76184..77194)
                     /locus_tag="PC523_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2473779.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVEVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            complement(77213..77995)
                     /locus_tag="PC523_000208"
     CDS             complement(77213..77995)
                     /locus_tag="PC523_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2473780.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            complement(78054..79190)
                     /locus_tag="PC523_000209"
     CDS             complement(78054..79190)
                     /locus_tag="PC523_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDF2473781.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            complement(79898..80365)
                     /locus_tag="PC523_000210"
     CDS             complement(79898..80365)
                     /locus_tag="PC523_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HDF2473782.1"
                     /translation="MSISNVREYFKQFGKDDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLIPEEVLEFTGHAIGGVCPFG
                     LKNSLKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            complement(80444..80986)
                     /locus_tag="PC523_000211"
     CDS             complement(80444..80986)
                     /locus_tag="PC523_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473783.1"
                     /translation="MKKFIKVSFFIFGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDIGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGINKCSSYVNIDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYVVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            complement(81038..81724)
                     /locus_tag="PC523_000212"
     CDS             complement(81038..81724)
                     /locus_tag="PC523_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2473784.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLMSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGLIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDMANAKYINLYSMTFLTIIIFIISMYFSI
                     RISKKNKFNY"
     gene            complement(81724..82584)
                     /locus_tag="PC523_000213"
     CDS             complement(81724..82584)
                     /locus_tag="PC523_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2473785.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     EFDRERFDEYLNKFDIEENMHIKNLSKGQSEKLMLSSALSHNAKLLILDEPTTGIDPI
                     VRSEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            82718..83518
                     /locus_tag="PC523_000214"
     CDS             82718..83518
                     /locus_tag="PC523_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HDF2473786.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            83543..84112
                     /gene="yfcE"
                     /locus_tag="PC523_000215"
     CDS             83543..84112
                     /gene="yfcE"
                     /locus_tag="PC523_000215"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDF2473787.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YTESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(84370..85422)
                     /locus_tag="PC523_000216"
     CDS             complement(84370..85422)
                     /locus_tag="PC523_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HDF2473788.1"
                     /translation="MKIKSRKIIAFVLSLGIMMPSCVYADTISKNQNTEVDMNNYLQG
                     RKSPKEETLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKEFLEDMGVLVSRIGGADREE
                     TSLLLTKQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTETYIVGGEYSISKKLEGQLVNAERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
     gene            complement(85629..86855)
                     /gene="pepT"
                     /locus_tag="PC523_000217"
     CDS             complement(85629..86855)
                     /gene="pepT"
                     /locus_tag="PC523_000217"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDF2473789.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEELNVITYVKDSPELVEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFNVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIATEISEMFPADERPETTEGYEGFWHLNSIDGNVENVS
                     MSYIIRDHCREKFEYRKSIMVENIEQINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELNIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            complement(86948..88000)
                     /gene="ytvI"
                     /locus_tag="PC523_000218"
     CDS             complement(86948..88000)
                     /gene="ytvI"
                     /locus_tag="PC523_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HDF2473790.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGGI
                     IALMINPISQKLENKFHINKGISTLVLSFLAVAIVSTVTTIIVINSVKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDIEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            complement(88148..89401)
                     /locus_tag="PC523_000219"
     CDS             complement(88148..89401)
                     /locus_tag="PC523_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDF2473791.1"
                     /translation="MLESPDNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            complement(89532..93059)
                     /locus_tag="PC523_000220"
     CDS             complement(89532..93059)
                     /locus_tag="PC523_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2473792.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNLQLQSLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQNSYEESKTVYEEQLKLEKNELRKNELDLKNGEIKEKI
                     ALVENDSNAKKVDPYIGSVQNLEKKIDEDSFTVSNLEKKLVILNQELEVIKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDKELKELKESGINLNKTKVELEKVKQMS
                     ESRKDAVTKSIKEVEGKINKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKINKLEEI
                     LKETEDINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KADNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEQLK
                     YLNLASELRRELKENMPCPVCGSKHHENHNITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKLSGYLSIEKMKTKELEDVKGKLGDIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYIGLKTITKVSNLLSKVKEIRENEKELELLSIGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYLEKKLSRDNKYKEVINITKGDLAKNLLHSVEEDICKILEQEESSKK
                     KLEQQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     ADEVKKLIEEILEHEEEQKLLAFKIKSSKEKLNGRSVKKESFEQLKDEIYNLKVEIGN
                     ISKNIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            complement(93046..94257)
                     /gene="sbcD"
                     /locus_tag="PC523_000221"
     CDS             complement(93046..94257)
                     /gene="sbcD"
                     /locus_tag="PC523_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HDF2473793.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITCATQGCTKLNINGENVVVATLPYPSEKRLNEVFSSE
                     DDFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDKEIWSYFEINTEEVISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            complement(94301..98128)
                     /gene="addA"
                     /locus_tag="PC523_000222"
     CDS             complement(94301..98128)
                     /gene="addA"
                     /locus_tag="PC523_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDF2473794.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRISLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSAEYKKIVDISQACNKSWDETYKK
                     MSSMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISNIVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGYRNEFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFEMDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRTNNKESEIEEEEK
                     LDNIQLEARMVGKIIKDLMKVNEEGKLQKVYDKGIGGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYNEFKDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGVENSDTISQYEILKGKNFLDWIMPCVLKHRDLSN
                     LLDEVGLDAVFSVEHNSKWYGKLWDKNDILVEKKNNEEKESIEKILEKIDVDSPDSDY
                     YNEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEERDKISGAERGTIVHLVMEVLDLKNVSNVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFESNIGKRMLNAELINREKSIYAQVNMKDIYIYEKLINNDDKKLYDEESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            complement(98128..101595)
                     /gene="addB"
                     /locus_tag="PC523_000223"
     CDS             complement(98128..101595)
                     /gene="addB"
                     /locus_tag="PC523_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HDF2473795.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRMLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQAGFVSSITEMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDVNYTGYSKADMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSKQTENIKIKEFNNLYSEVEEIAREIVHLVR
                     EKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINVLENYVLANGIKGKKWFDEKWDYRITQSLSGE
                     ESEFELELKDKINEIKNRVLEPIVTLQEKLKGKNRVKEICRYIYEFLLDINMPETIES
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSQTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDEDILKKITVKSPTFNELINVIKNYDSDGYNKEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHRIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKARKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLSWRGIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGDNG
                     KYPPIKIVLENGQEVSLIGQIDRVDEFEEGENKYIRIVDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDKDININPAAILYCRINNPIAKFNEDKDDVEIQEAILKELRMKG
                     LVVKDSHIVKKMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVKH
                     AIKDLCEDMLSGEICIAPYKHKDGTSCDFCDYSAICQFDSTMKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            complement(101585..102415)
                     /locus_tag="PC523_000224"
     CDS             complement(101585..102415)
                     /locus_tag="PC523_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDF2473796.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDNKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMSLNGDTIQAKYDLVVIGNNNIDIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYNEEKHETNRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            complement(102524..104743)
                     /locus_tag="PC523_000225"
     CDS             complement(102524..104743)
                     /locus_tag="PC523_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF2473797.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILVLTYMNSAVNNFKGRIRKILEEHKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKNIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGQKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENRNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVEYVTKEFRQ
                     EECRHALEDMQIKTVPNGMGYKENPNPEHYNINIKWYDSWKFEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLVFEELGPNSLSKRRVINNIASIIDFILHCDD
                     IEKLIVVLDKVFIDTDNEEELKYFLEKLKNYSTEEIIYDFEFSKLDNDLIEFNEESDI
                     YLNFKRGINIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLASENI
                     NMDLEDVYNLLFDVKNKVFNHIIDVVYEINGYEPEPGSITICNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLGRLNRHIIKKRSN"
     gene            complement(104736..106649)
                     /locus_tag="PC523_000226"
     CDS             complement(104736..106649)
                     /locus_tag="PC523_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473798.1"
                     /translation="MEYLIDKSSLKECGNEPLDKYREILDKTKNNSRSILLLVPNNNT
                     KIQYERSLRLNYSEELKITTYISFVKKELLKYWPLIIENCDEINKKAVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIAKSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNSLSVYLYNQYLLNDDFYVKSLFKEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDMDYINEKIISKIK
                     SKEENIKRKEIGIEDLYLLPATIKLNQSSQLYNEMLDLISEKIISLIEEGVSLRDIAI
                     ISPINNSILEYQIRDSLIEKNIDVFNIKKDKKAVDYPYGNALVVATCMFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNMDEHSQYRDILQYIEEKKKSDLKIHE
                     FLIQFYIDKMLSLKDGKKNVGLCKKIIAESESFSESLSLLGMKKEEIFIEALKTAIND
                     YYSVVDIEELKSKDKIVITTPYSYIASNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAYKSEYAINGYIQESILYSI
                     LLKISHKGDENYD"
     gene            complement(106881..>108149)
                     /locus_tag="PC523_000227"
     CDS             complement(106881..>108149)
                     /locus_tag="PC523_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2473799.1"
                     /translation="SISKTTANQIKSTINATQTRLNGSNRYETSLLIAKEIDKNHDVE
                     KVYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTSKWLENEDLQNAYFIGGP
                     QMISTNVINKVNGITKDNVTNNRVYGADRHETNANVIKKFYTDDELEAILVAKSDVLV
                     DALAAGPLAASLKSPILITPKTYVSAYHKTNLETKSANKVYKIGGGLTSKVMSSIASS
                     LSKHNTTPADPGSNNGAKTVMIDPGHGGSAPGNSFGGMIEKDYNLNTSLATTEYLRSK
                     DINVIMTRDTDKTLSLGSRTALSNSLKPDLFTSIHYNGDNNRKGHGVEVFYKFKDKNG
                     GTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVLRTNNIPAVLVECAFLD
                     NEKDVSLINSPAKVKEMGIQIGKGIEDSLK"
BASE COUNT        34237 a        19396 c        11334 g        43182 t
ORIGIN      
        1 tatatttcat attattaaaa tagagtaata gtttaaattc tactactcta tctataatct
       61 atttcttaat tattcaatct caaatttagc tcctcatcat ttaaatcttt cataagattt
      121 ctaaaggtac taggtttgtt gcaaaagaaa gactatggca ttaaacactt atatatctgt
      181 ttctttaaac tgtaatatta atatttttta aacacgctga cttttgcttt ttctaacttc
      241 tctccatgat agctctcccc tatcaagagc ttttataaat atttctgctg aagctaaatt
      301 tgtagctatt ggaacatgat aaacatcaca aagtcttatt aaagcttgta tatctgtttc
      361 atgagcctga gatgtcaatg ggtctcttaa aaaaataact aaatctattt cttgtgttac
      421 tattaaagaa cctatttgtt ggtctccacc taaaggacca gatgctaatc tgtttatatt
      481 taattcagtt tcatccatga ttctttttcc tgtagttcca gttgcatata gtccatactt
      541 ttttaaaatt gattcataac ctatacaaaa tccgaccata gtatttttca tttggtcatg
      601 tgctaccaat gctatattca tatatctaat ctcctcaatt tatgtttaga tgtatttaaa
      661 tttagaaatt tattttcttt cttctcatac acacatttag tactaaccct aagttcgcta
      721 aagatgtcaa taacgaacta cctccataac ttacaaaagg taatgttact ccagttactg
      781 gtattaacgc aaccgtcata cctatatttt ggattatctg gtaaccaaac attcccataa
      841 caccaaccac gatgagggtt ccataaaagt cttttgcatc tcttgcaatt gctaaaagcc
      901 ttaataggaa tatcataaat aagattatca acactgccat tcctattaca ccaagttctt
      961 cacctacaac agcaaatata aagtcactat cttgtacagg taaaaagttt tcttgatttt
     1021 gacttccatt gtaaagtcct ttaccagtta caccaccaga accaattgca atcagagatt
     1081 gcataacttg atagtttcct tttagtgtta catcttctgg gtttagaaaa gcttctattc
     1141 ttaccttttg atggtctgcc atcattaaat acatcagtgg cattgacacc aatagtatga
     1201 ttattcctct ttttatcaac tttgaactaa gacctgctgt aaacagcatt gcaaatatca
     1261 tacaacaaaa tacaattcct gttcccaagt ctggctgtgc aatcaataat ccaataaatg
     1321 gaagagaata aactataaca ggcataactt cctttaatgt atttaattta tcctttttgg
     1381 attcaagtat ttttgcataa cttagtacaa atgttaattt gactatttca gatgtctgca
     1441 agtcaagtgg tcctaaatta atccacgacc tagctccacc ttttactgac ccaattccag
     1501 gtaataaaac gatggctaac aaaacaagac ttattatata aagagctttg taatacctgc
     1561 ctagaaaatt ataatcaaaa aataatatga ctataatcat tcctatccca agtacaaagg
     1621 ctaacccttg tttaatcaat tgattatatg aacctgttga attagcatgt gttgcactac
     1681 taagtatgac aagtccaaaa ataaagatag ctaatactgt aactataagt ttccagtcta
     1741 actgttttat caacttaatt gtcgacttat aattaatatt gatttttttc aaagaatcac
     1801 cgtctttcta tctttaaaca tataattaat aaaaggggca tataccaata aggatatccc
     1861 ccttttaagc actaattaca tacatctatc ttttatattc tttagtggta tattagcaac
     1921 taatgcagat acaggtttgt catcatgctc acctctgcat ttagacattt ttatttcaag
     1981 gccatcatct tctatttctg cataattagc tattactttt aaaatgtctg tttttatcat
     2041 ctctaaaagt tgtggtgaac aatctactct atcatgaact aaaactagct ttaatctctc
     2101 tttagcaaca gacttactag ttttagcttc gttagaaaaa actctaaata aatctaacac
     2161 agatgtatcc tcctttgcta cttagccatg ctaaacattt ttttaagtct accaaagaat
     2221 ccatcttcaa cttcaagatc aagtagagga acttcttcat ttagtatcct ttttgcgata
     2281 tttttgtatg cttgaccagc aagtgactta gaatcaagta tagctggctc tcctttattt
     2341 gttgatataa ttatactttc atcatcagga acaagaccta acaaatctat tgctaatatt
     2401 tctattatat cttgtttatc catcatgtct ccacgcttaa ccatatcatt tctaattcta
     2461 tttataacca atcttatctc ttttatttca tttgcttcta atagacctat tattctatca
     2521 gcatctctta ctgctgatat ttctggatta gtaactacaa tagctctatc tgctccagct
     2581 actgcatttt taaaaccttg ctcaatacct gctggacagt ctattattat atattcgaaa
     2641 gattctttta gtttctcaca caggtcaatc atctgttcaa ctgagacagc atttttatct
     2701 ctagtttgtg ctgctggtaa taaatataga ttatcaaatc ttttgtcttt tattagagct
     2761 tgcttaagcc tacaagttcc ttcaactaca tctactatgt cataaactat tcgattttca
     2821 agacccatta ctacatctaa gtttctaagt ccgatatctg catccactac tactgttttt
     2881 ttattttcta ggcttagcgc agttcctaag tttgctgcgg tagtagtttt tccaacccca
     2941 cctttaccag atgttataac tataacttcg ctcataatac taccccctat ttttctttct
     3001 atttgtctaa ctttgacaaa tagctttcga ttacaattct tccattactt acaaaggcta
     3061 tttctgggct aatttctgat tcactttcat aattttcttc atcatctgga gcttctgcaa
     3121 tattatctgc tatttgtaat attttaggat taagatttcc agcaacaaca tatgcaaatt
     3181 cattaccttc tgcacctgca tgtacaaaac cttctatttt gcccattaca aaaacattac
     3241 ctcctgctac aaccttagca ccagcattta tatctcccat aacaactgta tttgattttg
     3301 aaaggacctt tgaaccaggt ttcatatcat tcaatataac tacatcacct tcaaattcaa
     3361 tattttcacc agatcttagg gtattgacat attttgttgg aaaattacta ttttcttcaa
     3421 ctttttgatc ttcaacaaat tccacatcaa atctagaagt aattccttct ttcagttcta
     3481 gaatttccat gtcagttaaa caatcactgt taattttact aattttagcc ccattaaaaa
     3541 agccaacata tgcttccaat ttgtttatta ttttttcctg tatttcctca aatggagctt
     3601 ctctttttat atttactatt atccctcttt tattaccctt gaactctact agttcttgac
     3661 tacaaatttc tcttaaagac ataacaacct ccagagtgat atatcattta tattcttcaa
     3721 atacgcttta aaccctttta ttttttctat ataaagctaa agtttataca tggattgagc
     3781 tatctattaa tttgtgactc gaaatttata ttttcgtcag aagaattgtt aacatcattc
     3841 gtcttgttgt catttttact attagttggc tctaatccca tataagttcc aataacttct
     3901 ctagttggta aaagtgagaa tacacttgaa tcaccttgag gaattacaca tacaacagct
     3961 atttctgggt catctgctgg agcaaaagca acagtccatg taaaagaacc ataatcttgc
     4021 ttaaatctat ctatttggtc atctgttatt ttgggattta attcttttat agcttttctt
     4081 agatatgaag aatttacctt atcggtgtcc tctaatctaa cttttactcc atcttctaat
     4141 tcttcctcta atttttttct ttcttcgtct ttcaaatctt tattttctaa tttctttttt
     4201 atttcctttt ccttagctaa agaaagctct ttttcttttt ctgctttcat cttatcagct
     4261 agtttaattg catcatttag gttaacatta taagatgaca tatgactttt taaatattca
     4321 tactcattat ctgtaggtat tttcccactc ttctcagctg taccagtttt tgctgctaca
     4381 tctattggga aatcagcaaa ggcactctta gctgtacctt gtcttgcaac caatttcata
     4441 ccttttgtta attcttttaa gttatctgga tttttaaaag gtattttttc tactttcttc
     4501 tggtcattaa ttttaactga tgaatagtca cttgaaacag ctctatcaac tacagataat
     4561 tctactagat tacctccatt tgctatggca gctacatatc ttgatatttg agctggtgta
     4621 taagcatttt caccttgacc tatagctaag ttgaatgtat ctgcagtact ccattttgca
     4681 aagttaaaat aactgaatac tgctaaatct gctacatctt ctattctatc ttctttaaca
     4741 ttcattttct ttagcctatt cattgtttca actctaccag gagttttctt ttctgctgcc
     4801 caacttacaa tctcattaat tcttttctca tattctttag gatttttttc tctagttata
     4861 tcaacaaaat catttgccat ttctctttct aaattcgact ttagaagagc ttgtgttgat
     4921 ttcaatttat cttctgtgct aggaacttta cctgctcttt cttctacttc atcttgaaga
     4981 cctgtatttt gatcaagtcc aaataatttt gcatactcaa gtattttatt tgcattcata
     5041 tctataccaa tgctttttcc atctggccat gttttacctg taccaattgt tgccatatat
     5101 atattacaag actcttgtat tgctttatat aagtttgtca taccatggtt tcctctacca
     5161 tggttccata catagtcacc aaaagatttt ttacctaaca ttataacccc agggtcattt
     5221 atagtaaagt ttggattcaa accattctct aacgctgcca ttgaagttac catcttaaat
     5281 gaagaacccg gctgaaattc cccttgtgta accaagttta aaagtggact tcctgctagt
     5341 aagtcatttg gattttttgg ttgaagtgct ttataatctt ctgtagaaat tccttttgca
     5401 aatttatttg ggtcataatt aggataactt gatgaagcta atacttctcc atttttaaca
     5461 tcaatagcta taagagctgc tgattgtgct tttcctgcat atgcactaat tggtttgtcc
     5521 ccaaatttac ttttaaaggt accaccctta gaagcaactt cgattatctg ttttaatgca
     5581 ttatctgaaa cttcttgtaa atctttatct agtgtaagat atacagtatc tcctgattta
     5641 ggttttttag actcaatttc tttactgatt ctacccagtg catcaacttt aaccatttta
     5701 taaccatcag ttcctcttaa tctactttca ttactcttct ctactcctgc aagacctacc
     5761 atatctccag tttcataccc cttttgaagg tatgattcta tttgagtgga tggcattttt
     5821 cctacatatc ctaacatatg agaagctaat gacccattag gataatatct tactggttct
     5881 actgctattg aaacaccaac aaggtccata gcactctctt caatttgagc aattgtagtt
     5941 tctcctacat ccttcgcaat agttactgga ttatattgag agtatccttt tgattttatc
     6001 aaatctctta caaccattat ttttcttgca tcttcatcac ttaagttttt atcaatttct
     6061 aatgcatcac tatttcttac tttttcaaac gcttctttgg cacttagttt agtttctttt
     6121 atcttataac tagccaacca atctctctta tctctttctg ctgtgaacat ccatttacta
     6181 actaaaatag gtggataata tccattttca tttagttttg cctgtaatct tgtcgcatcc
     6241 aatcttttat cttcttgaga taatattcca gctgatataa gtttatctac caagtaataa
     6301 aaactttcct tagcattata atcatttgga ataccatttt ctgacttata ttccctaata
     6361 tctctgtcat atgtataata gtactttcca ttttctacat atataggaaa ctcatctaca
     6421 tattcttcac catttctctc cagcaaattt ataagtttta atgatatttc atttgctctt
     6481 gaatgcttat ttgcatcttt tttattaata tcgttacctg aaacttgaac agtaaataag
     6541 tttttatttc ctgccaacag tcttccatat ctatctttta tttctcctct tggagcttct
     6601 attgccaact ttttatatgt tttattctca gctaactcat tgtaatattc atatttaaat
     6661 gtagtcatat agattatttt tactaaaata gctaaaaata tcacgctcat gactatctta
     6721 attattttta gtctatcaac ttgtttattt tcttccatga aatcaccaac taatcttctt
     6781 taagttttaa aactgctctt ttagataagc gatatattac taaaccaatt aaactattca
     6841 ctattggtgc tactagtaaa ccttttataa aaagtgttga tatattgtag ctttgataaa
     6901 ttatactaga tgtacctata ttgacaattg aatcaattaa actgcctata aaaattaaaa
     6961 cgaaagtaat aatataacta cctttatata gctgatctct catgtaccca attgcatatg
     7021 caataataaa caaaattaat gcatttgcac caaaaattcc accaacggtt atgtctttaa
     7081 taagccctat tattgctgct ataatcccac cttctaattc atccaaataa agagagatta
     7141 tggttacata tataagaact aaattaaagc ttatgccaaa aatatctata taatttgtta
     7201 tactattttc tatagtcata agcacaatcc ccaacagaca aagtaaaact tttttcattg
     7261 tttttatccc ctttatccta tatttcttgg ttctatgact acaacgtcat ttatattctt
     7321 aaaatcaaca taaggtttta caactacata ttttaatgac ttatctttat catcaaccac
     7381 tttttcaact tcgccaattg gtataccatc tgggaagaat ccaagacctg atgttgttat
     7441 tatatctcct tgaattacat tatatgatga gtcaaacata tatccttgca aatatccttt
     7501 actattataa ctatctttat tatctaaagt agtattttga gtaattgtac ctttagcatt
     7561 ggcatccttt gctagtttaa agcttacaga tgccttagaa tccaatagtg aaattgcctt
     7621 actataattg ttggaaactt cataaactat gccaactaac ccactaccat tcataactat
     7681 actttctttt tttacacctg aattttttcc tgcacctatg acaaaactct cataccagtt
     7741 tccatcattt ttactaacaa cacttgttga aatacttgtc gccttatact cttcttgtac
     7801 aaagttaagt gtctttttta actcttctag cgattctgtt ttatctagtt tggcatttaa
     7861 agatatcata tcttttttaa gtttttcatt ttcttcttct aattttttta cctttttaga
     7921 attatcttta aaatttgcta tgtctttaaa accatttttg ataaaagaaa atccatcatt
     7981 tattccttta cctacacttg taatagcatc tgaagctact cctaaattta ctgggctacc
     8041 actagaaaat ttaccaattg atatacccac aattccaatt aaagtgatag caactacgcc
     8101 cgttgctatc actttaacat taatcctttt tttctcattt ttgttcttgt caaatctcaa
     8161 ggccatcacc aacttctaat tcttagtatt catcattaat actttttcaa agatttcttg
     8221 gtcttcaaca gattttcctg ttccaagtgc aacacaatca agaggtgttt ctgctatttg
     8281 tacagacata ccagtttctt gtgtaataag cttatcaaga cctctaagca aagctcctcc
     8341 tcctgttagc attattccgt tttccattat atctgatgct aattctggtg gagttctttc
     8401 tagtgtagat tttattgcat ctactattga acttacaggt tcttttaatg cttctctaac
     8461 ttctgtagag catatttcta ttgttttagg taatcctgag attaaatctc tacctcttat
     8521 ttgcatattt acttcttcat catctttaaa tgtagagcct atgttaattt ttacattttc
     8581 agcagttctt tcacctatca taaggttata ttcttttttt acataagcca ctatagactc
     8641 atcaaattcg tctccaccta ttcttataga tttagcagta acgattcctc ctaaagatat
     8701 tacagctatt tctgaagtac ctccaccaat atcaactacc atattacctt ctggttcttc
     8761 aacttttaat ccagccccta tagccgctgc cattggctct tctataagga atgcatcttt
     8821 agctcctgct tgtcttgcag cctcttctat agctctcttt tctacttctg taactccaaa
     8881 aggaacacat atagctattc ttggacttac aacacctttt ttatcagccg ctttttgtat
     8941 aaaataacta atcattgatt gagttatgtc aaaatcagct attaccccat ctttcatagg
     9001 tcttatagct actatatttc ctggtgttct acctatcatt tttttggctt cttcacctac
     9061 tgctaatacc tctttactgt catctcttat tgcgacaact gatggttctc taacaacaat
     9121 accttgtcct tttatgtaaa ctaatgtatt tgcagtccct aaatctattc ccatatcctt
     9181 tgtcatctta ttaaaactaa aaaaaccttt tttttctttc tttttttcct tagccatgat
     9241 tttactcctt cctactatta agaatcatgt ttaaaacatt ctcttcttat tttatcacat
     9301 ataaagttaa attatcatat tttctttaaa gctaaagtat aatccatctc caataattat
     9361 atggtctatt atttctattc caattatttc tccacattta ataagtcttg atgtgatatt
     9421 tttatcttct cttgatggtt ccacacttcc tgaagggtga ttatgcatta ctattatctt
     9481 acttgcactt cttcttatag cttctttaaa aacctctcta ggatggacta atgacgagct
     9541 taaagtccct acagatacat ctacatcaga tattatctca ttttttgtgt ttaaaagaac
     9601 cgctttaaaa acctcttttt ttaaatacct taggctatcc atataatatc tctgtatatc
     9661 ccaagggttc cttattttat actttatagg tttaaaacta gctactctag agcctagttc
     9721 taaagctgct ttaatttggg ttgcttttga taacccaatt ccatctatat tacacagctc
     9781 ctctattgtc atatcttcta aatgtctaat accctgaata tctctattta ttatatagtt
     9841 ggctaattca atagcatttt tattcttgtc gccagttctt aaaagtatcg ccaacaactc
     9901 agcatttgat aagcttttta cccctttggc aagcatcttt tctctgggtc tttcctctgg
     9961 tgccatttct tttaccttaa tggttgaatt aaatgacttt ttcagacaaa tcacactcca
    10021 taaaaaagat ttatactaaa atattttttt aataggtcgc ttaaccttga aattggaaga
    10081 ccaactatat taaaataatc tcctctaatt tcttcaacta acaaagctcc atatccttgt
    10141 ataccataag ctcctgcttt gtcaaatgac tcattagttt tcagataatc ctcaatatct
    10201 tgctctgata acgttttaaa tttcacattg ctgattacat agtcaattac ctttttatct
    10261 tcgcacaagt ttattaagct aatacctgtt atgacttgat gctctcttcc agatagtctt
    10321 tttaacatat ctcttgcaca agactcatct tttggttttc ctaaaatagc attgtctaaa
    10381 acaacaactg tatcagctcc aataacaaca tcttcattat gttcagatgc tacagacata
    10441 cttttttcaa aagctaacct cataactaaa tgttttggtg tttcaccttc caatataatc
    10501 tcatcaattt catttttaat tatatcaaat cttacatttg tattctctag aatttctttc
    10561 cttcttggag aggctgatgc caagattata ttcatttatt cacctcattt gtacgtaatt
    10621 tgtacctatt tagtctatca ctatattgta aaaaattcaa cagaaatgtc gaaccttttt
    10681 tgttacacta ttgttatatt tattaccatt agaataaata tacataaaat agaggtatgg
    10741 aaattattcc atacctctgt tttatgtact cattagagat atggaaatta ttccatatct
    10801 ttattttgtc catattacaa aattttaata gctttctttg gacatttttc catacataaa
    10861 tggcatccta cacatttatc tgcatctacc ttatgttttt cttttaattc tccctctatt
    10921 gcatcaaact tacattgttt tttgcacact gtacatccaa cacaaagttc ttggtcaata
    10981 gtaactttct ttttcttagt tatgtcacct gatattactt ttgtagggca ttttgctaca
    11041 cattgcatac aacctacaca tttattaggg tctatcttag ctattttgtc ttcaaatatt
    11101 attgcatcaa atttacaagc ttttacacac attccacagc caatacatcc agcagtacat
    11161 ttttccttaa cagctttacc aaactctttg ctattacact ctacaactac ttcttgactc
    11221 tctggttttg tgattattat acccttaggg caaacttctt tacattttcc acaatttaca
    11281 cacttttctt catctattac tgctactcca tcaactatag aaatagcatc aaatttacaa
    11341 gcatctttac atgttccaag acctaagcat ccaaatttac acatcttggc acctgagttt
    11401 aaaacatttg ctgctctaca atctgatatt ccttcatact catatttatc ttttgcactt
    11461 gaacaagttc ctttacaaat aacttttgct acttgtttct cacctgcttc agcgcttatt
    11521 cccattattt ctccaacttt tgcaccaaca tctgctccac ctacagggca accattaact
    11581 ggtgcattgc cttcaactat cgcatttgca agacctccac aacctggaaa tccacaacct
    11641 ccacagttgg ctcctggaag tactcctagt atagcttcca ctctctcgtc tacttctaca
    11701 gcaaactttt tagatgcaaa atctagtact atcccgaaga ttaagcccat aattcctaaa
    11761 actaatacag ctgtaagtat caccattttc acccctcttt tttatagctt tataagtcct
    11821 gtaaatccta agaaagctat tgacattaaa cctgctgata taagcgttat agggaaacct
    11881 ttaaatgctt ctggtatatc agctaattct agtctttctc ttatacctgc gaatattact
    11941 attgctaaag taaatccagc tccggctcca aatccattta ttattgtttc tactaaatta
    12001 taactttttt gaacattaac tagagctata ccaagaactg cacagtttgt agttataaga
    12061 ggtaaataaa ctcctaatgc ttgatataaa gatggactca tcttttgaat taccatttca
    12121 acgaactgaa ctatagaagc tataaccaat ataaaagcta tcgtttgtaa gaatccagtt
    12181 ccagttttta ctaataaaat ctgtataaaa taagttataa ttgaagctag agttaaaacg
    12241 aatgtaactg caactcccat ccctactgct gtatcaacct tcttagaaac acctaagaaa
    12301 ggacatatac ctaagaattg agatgttata acgttattaa caagaacgat acttaaaaat
    12361 aaaagtatta aattcattta ttacacctcc actatgcttt tttacttctt aatttattaa
    12421 atcctgcaaa taagaatcct aatgttaaga atgcacctgg tggtaatata aatattaata
    12481 ctggttggaa agatgctcca aataatgtca ttccaaataa actaccattt ccaagtaatt
    12541 ctcttactgc acctattatt gtaagtgcaa cagtaaatcc aagcccttga cctacaccat
    12601 caactattga aggcatagcc cctgttttga aagcaaaact ttctgcacga gctaatatta
    12661 aacagttaac aactattaat ggtatatata taccaagagc cttatcaaga gcaggaacat
    12721 atgcttttaa aagcatacct actattgtaa caaatgtagc tataactact ataaatatag
    12781 gtattcttat cttatctggt gtaatttttc ttattaaaga tataactaaa tttgcacata
    12841 ttaaaactgc tgctgttgaa agacccatac caattccatt tattgctgaa gttgtaacgg
    12901 ctagtgttgg acacatccct ataacttgaa caaatgttgg gttctcatct attagcccat
    12961 ttttaaatac tttagctaaa ttcataatta accctcctta tttattaaga gcactattga
    13021 aaacatctat agcagcattt actcctgtag taactgctgt agatgtaata gttgcacctg
    13081 atattgctaa tatttgatta tctgctgttg tacttccttt tgctacttca agaggttcag
    13141 cagtttttcc tttgtattga cctttaaatg catcatcctt agctttcgct ccaagacctg
    13201 ctgtttctga catgttacca atgtcaacac ctgtaacttg accttcagat gagataccta
    13261 ccataacttc aatttctcca ccatatccac ttggtttagc ttttaaagtg tacccaacca
    13321 cttcagaacc atctaaacct tcatacactt cagctataag accatctcca aggtctttat
    13381 atgtactttt atccatttct ttaaattctt ttgcttctgg aagtactgct tttctaagtt
    13441 cattatttgc ttgtatattt ctttgttcta ttactggagc tgtaacctga tttgtagctc
    13501 caagagctaa tgcagctatt gcacttatag caagtaaagt accacctaat ctcaccatac
    13561 tgttcattat ttagccacct ccccaaatac tctatttcta acaaacttat ctataagtgg
    13621 agcaagacag ttcactaata gtattgagta agatacacct tctggatatc caccatacat
    13681 tcttataaca gttgttataa gaccagctaa tacagcatat attatttgac cttttttagt
    13741 cataggcgaa gttgtataat ccgtaagcat gaagaatcca cctaacataa gacctccagc
    13801 aaacagttga tatattgcga aagttatatt aaatccacct aaaaggaatg ttaatataaa
    13861 tacagttgct atgtaaaata ctggtataac ataatttatt atgcctttat acattaggta
    13921 tactccacct aataataata atattgctga tgtctcacct atacatccac caatattccc
    13981 tatcatcata tcagcaagac taattccatt gctagaaagg tccgctagtc ctgctgctcc
    14041 atttttcaaa aagcttaatg gagttgctgt agtaactgca tctaaatttt tacctatcca
    14101 gttgacacct ggttgagtcc aagcagtcat tgctactggg aatgatgcta ataaaaatgc
    14161 tcttgcagca agtgctggat tgacaaagtt acatccaatt ccaccaaata ccattttaac
    14221 tactaatatt gcaaatattc ctcctactag acacatccac caaggaagtg atgctggtac
    14281 gttaaatgct aataataatc ctgttacaac agctgaaaaa tctcctatag tacttttttg
    14341 cttcgcaaat ttttgatata aaaattcagt acctactgta ccaagtatac aaaaaaacat
    14401 agcactcaaa gcattaagtc taaagaagta tactcctgct actgctgctg gaaggagtgc
    14461 tataataact tgtttcatta tatatgaagt atcttcatta cttctcacat gaggagaaga
    14521 tgatactatc aacttatttt ccatcgtttt tcccccttaa ctttctttat ttctgagcag
    14581 cttgctttct tctctttgct cctatttctc tctttgctga tcttatagat tgaagtaatg
    14641 gtcttcttgc tggacaaata aatgaacaag acccacattc tatacaatcc attgctctat
    14701 gatattcagc tgtgtcatag tcatctttta aagaatatgc acttatgaaa agtggttgta
    14761 agaatgatgg acatacatct gtacatcttc cacatcttaa acagttttga acatctggag
    14821 tacgtgattc ttcttcatcc aaacataaaa ttcctgaaga acctttgtta gtcgctattt
    14881 cagtagtata ttgagctatt cccatcatag gtccacccat tataacctta ccaatcttct
    14941 tatcttcttt aaatccacca cattgttcta ttatttctga aactaatgtt ccaacttttg
    15001 ttattaggtt ctttggctct ttgatgcaac tacctgttac tgtagtgatt ctttctacca
    15061 aaggcatacc tgttttaata gacttagcta tttgtgctgc tgtggcaacg ttgtcaacaa
    15121 ctgcacctgc tgctattggt aatccaccag atggaacttc tttgcctgta catgcgtaaa
    15181 taagttgttt ctctgctcct tgtggatatt tcacttgaag accaacaact tcgatttcac
    15241 tataatcttt agctacattt tgaattgctt ctatagcatc tggtttattt gtttcaattc
    15301 ctataaatcc ttttttaaca tctaatacct tcattaatgc tcttaaacca aatacaacat
    15361 cttctggatt ttcaaccatt aatctgtggt ctgcagttaa atatggttca cactctgcac
    15421 catttaatat aactacttct gccttgctat ctggtggagg agatactttt acatggttag
    15481 gaaatgtagc tccacccata cctactattc ctgcctcttt tatgatacct actatgtctt
    15541 ctttgctcaa atcctctaac tgaccttttg gttgaacact ctcatgtaat tcttccttaa
    15601 agtcattttc gataactaca cattgagctg aaccacctgg tacttcatgt tgttcaatag
    15661 caataacttt acctgatact gaagaatgaa cgttacatga aacaaaacct tgttgttcac
    15721 ctattttttg cccaaacttt acttcatcac caacttcaac aatagtttta gctggtgccc
    15781 ctatatgttg ctgaagagga atgtaaacta tctttggagc ttgagctttt tcaagtgcct
    15841 ttgtgttaga gtactctttt ctatatggag gatgtatccc tcccttaaaa gttaagagtt
    15901 tcattgtttt acccccttat ctataaaaaa acattttttc tgccttctat aagtatacta
    15961 tatatattaa aaaatcttaa caattgttaa aaaaatatcc gtatttttat aatttatttt
    16021 ataaatcaat gtctctatat taagtcaaat cgaggaaaag gattgcttaa ttaaacaatc
    16081 cttttcattc agctttctaa agtccaaatt ttctgtattt tattttatag aatttataaa
    16141 catagagtac tcctgcatag atgataaaag tagactttta cttacactat atttttcttc
    16201 atttgctgct tttgaagctg tatcaatttt ttcattcaca ttctttacat actctattaa
    16261 attatcttta aataaacgca tttcagaata atcaattttt tcagcttctt ttgatatatt
    16321 ttgtgatatt gcctttctat ttgtcaaaat agtattatat ttttccatat ctacattatt
    16381 ttcagcattc gaccaaaaac ttgattcttc tttaaagttt gttattaatt tatttaattc
    16441 tttagatata ctatctatat aagcatagtt acttgtatac tctaaagata acataggtgc
    16501 atctaagtgt gataaaaatg catctggaaa cacctttctt acactgctta tctgctttct
    16561 aacatcattt tcatcaaaag aggaatatgt ctgtattttt tttactccat ccttctccat
    16621 aactgaaaaa ggtatatcat tgttaactaa tgatgtctgt atttttttca aatcatcatt
    16681 attatctatt gcagcaactt gaatagtgta catatcccag ccttctatag tagctaattt
    16741 aacttcttct ggctctttag tgttatttac cgctttttct ggattagttt cttgtttatc
    16801 atctgaattg gtttgtgatt tagatttttt tccgtttttt attgaattta attcatcaga
    16861 tatatcttta taagttatat ctgatgaatt atttagaaat ggtattttag ccgatacata
    16921 ctccaatatc ttgctatctt ttatttttgt ataagaataa tagccaatta gacacaagca
    16981 agcaatcgtt ataacatatt tacgtaaatc aatcttatgt ctattattat taaatcctct
    17041 gtatatcgtt tttcttctat tcattagttt gtccctccca aaattatatt attaataaag
    17101 tatattttta agagtcacaa tttatgaata taaataatta tttattctaa ttttttaata
    17161 agaaattgta cttactaaac aaatcttctt gcaccaagta atactttaga ccaatatgaa
    17221 ctagttaaat tatccacttt aaccttatta ggattagttt gagtaccact acaatgaata
    17281 aatttactat tgcctacata tatacctaca tgattagttg ttccatcacc atcagtatca
    17341 aaataaacta aatctcctgg tgcataattc cccatagaaa ttgcacttcc aaaattagcc
    17401 tgttctctag aaactctagg aatagaaacg cctacagaat gtttaaaaac atactgtgta
    17461 aatccagaac agtcaaattt atcaggtcct gaagctcccc attgatatgg tattccaata
    17521 agagtatatg caaaatctac gattgttgaa ccttttgctg tagatttatc tgaatttcca
    17581 gtaaatgatg gctttgagtt attattagat tgagaatttt gattagatga tgaatttgaa
    17641 ttttcttttg taccttcatt agtctcagaa atataactgc cactcaccca tccagttgtt
    17701 ccattcgata gttttacctt ataccaacca ttatttttag cttttaattc aacaacatct
    17761 ccaccattta attttcctat tatagaatag cttgtcccag gaccacttct tacatttaaa
    17821 ctcacagtag atgttacctt accattttta ccttcaattg attcttcgct agatggtttt
    17881 ttatcagagt tattagtact atttgaatta ctggaattat tcgctccaac atcttctgac
    17941 gttttagaga tatattggct actagcccat ccagtaaccc catttgatag ttttatttta
    18001 taccatccat tgctttgttc tagtaacttt actacttctc cattatttgc tttaccaact
    18061 aatgaatagt tagtacctgc accacttctt acattcagtc tagagctcac atttacttta
    18121 ccatttccac tgatagttgt tccacttgaa gttgaattat ttggatttga tgtaccttca
    18181 gatgaaccac ttgttgatat gtattttgca cttccccatc caacaacaga gcttgatacc
    18241 cttactttat accatccatt agatttttct aaaatctcta cagtttttcc cttgtacaat
    18301 tttgctacct tatcgcaatc agagcttggt ccacttctta tgttcaaggc acttgctgtt
    18361 actgttccct tctcaagagc acttgcattt atggagctaa cagatactgc tactgcccca
    18421 attcctaaag ccgctatagc tttcttcact actatcactc cccccaacaa atttttatag
    18481 tcataaatcc ataacattat tatccatttt tagtacctat aatttaatga taacttattc
    18541 tcttattaat tgtaaagaaa ttataaacat atccttaaaa ttaatgattt ttaggaagta
    18601 tttcaatcca ataattatct gggtccgcta taaaataaat tcccatactt tcattttcaa
    18661 aacatatgca atctaaatct ttatgaagtt tttttgcctc atcaaactcc tcaacttcta
    18721 aagccagatg aaattcatta tctcctaaat tatatggtct gtcccaatct ctaagccaag
    18781 ttaattctaa tgtatgacta gtaatgccat ctcctaaata aactaatata aaacttccat
    18841 cttctgcttc cttacgcttt acctctttaa gccctaaagc ttttttataa aaatctaaac
    18901 ttttttctaa gttagtaaca ttgaaattgt tatggcaaaa attaaacttc attttgaaat
    18961 cactctccaa tttttatttt tttatattat atcattatta taaatttatt taaatcatat
    19021 taagctaaaa atttgaacga tgataaacaa ttaccagata ctggtaaatt atttaaagga
    19081 atatgtcaaa aaaagaataa gctctttcaa atgcataaaa gatttatacc taagttatac
    19141 ttaatcatgc aaattgacag aacttattct ttaaaacttt aacttttaag tttttctttg
    19201 atgatataat ttattacttt ttttctaata tcttcagcat tataatcatc ttctacttct
    19261 atatccaaaa gttttgctat tgacctcata tcttctctag tctttgacct atagcttgat
    19321 attaattcat cttttatttt ctcaggctct aaaagatatt cttcatctcc tcttttgaaa
    19381 acaacgtatt tttgagcata tgaacttcta ttgtagtata tatatcttga tatttttctt
    19441 aaaatttgat tatatgaaag tcgcttgtta atatcaagtt ttaagtactc tgaaaaagca
    19501 aatagctctt tttttgattt aaataatgct tctttcttta tagtccttga tattgaagtt
    19561 acactttctt gatatgcaca cttttttaac atttttaaat atactctatt aagaccgata
    19621 ctttgattga atttttttat attattatta ataatcttat ccaagttacc ttcaaagtat
    19681 ttttttgctc ctttttgtga tacataactc tcaagttctt tgtgggccat atcaagtatc
    19741 atttttttta aatcctcttt atatcctcta gattccatga tactccctcc cttagctatt
    19801 tatagtatat tcaaaaagct caagaaatgt gagtcattac aaattttttt tactttttat
    19861 gtatatatta atttaaaagg ggagataata gtaatgtaaa atcaaacata ctcaatctcc
    19921 cccattttta gacctctatt actcccccaa tagaggtctt tttttagttt attaacttct
    19981 attcaattcc ctttagttca aatcctaatt ctgctattac ttcttttaat ttttcaatag
    20041 atacactatc tttcatatct acagatgcac acttgttttc taaatctaca tctaaaactt
    20101 caataccatc tatatcttct gttaatgcag ttttaagatg atttacacaa tgaccacaac
    20161 tcataccttc tactaatagt ttcttcttca tttaattacc tccttatacc ctataagggt
    20221 attttatcat atagtttata cttttaaaat agaaattaat catttaattt gattcattcg
    20281 attttatatc tagattagat attccctcat atacatcaaa atcatttgga gtaattttac
    20341 cttttaatct actactactt acaatatagt tgtttctaac agctaaactt ataaatgttg
    20401 cattatcttt tatatatgtc tgtacatctt tgtatgcttt ttttgtttca atttcagatg
    20461 tagaacttgt aagtgcagac atatatccat ttagcttatc atcagtatat ccactactag
    20521 ctataatact acttgcatct ggtatacttg ataatttcca tccaactaat gctaaatcat
    20581 aatctttaga attaagagca ttttccatgt ccttatctga cagctgattt actgtactat
    20641 ttattcctat agcctttaag ttttcattta ttatgtttgc agtttttatt ctctcactat
    20701 tatctctgtt tactattatt tttaagttca tctcagaaag ttttttcttt acttcctctt
    20761 tttctctatt ttgtttctct tcagcttctt ttttgatgct atcttcttcc gcttttttag
    20821 cttttgcttc ttcttctgga gtaagcttct ttaaattctt tttagctggt tttttctgat
    20881 tctttatttt attgtcattt tgatttactg gatttacatt tgcatattct accttagcaa
    20941 ggcactctag tgctttatct ttattaaact ctaaatcctt catttcacta ttatagtatt
    21001 ttgattttga attaagtgga aaattaactg gagtagcatc atccatataa ccttcactaa
    21061 taattctatc tttatctata gagtgcgcaa ttgcttttct aaaattgaca tctttaaaaa
    21121 aaggattatt ataattaaat aatacacact cataatctct accttgatat tttgttatat
    21181 taaactcttt ttcttgaaac ttacttaaat catttaatgt aacattggtt atatcactat
    21241 cgagagccat aaccatagat gtccttgcat cttcattagg caccatacct acttttatgt
    21301 tcttcatggt ctttggaact tcttcatagt agtctttgtt tacagataaa accattcctt
    21361 ctctttctgt atatgattct attttatatt tcccatttcc tatcatatta ttattcttgt
    21421 catttacatc ttttgaatct aaatgacttt gagatactat tggaaaaatt aaactatcaa
    21481 ttgagaaagc atatctagct ttaaatttta ttgtaaaatc tttatctgat ttaatattca
    21541 cagaagctat atttttggta aattcattat atggactatc tgcacttttt tgaattaaac
    21601 caatagtata gtttacatca tttgaagtaa catcttttcc atcatgccac tttgcatctt
    21661 tcagctttat atctatactc attccatcct gagctatatt atactcctta actaattgag
    21721 gaattacatt gtagttctca tctatagtaa ataaactatc ataaactaaa ttcattatat
    21781 aaccaacaga cttatcagta ttagttatag gatttatagt ttttggagtt accattgtta
    21841 agtttatata cttagcatct atagaacttt gactttgtaa acttgaatca ctactacttt
    21901 gttttgtttt actattacta caaccaacca ccataagagt tatagctaaa acaactgtta
    21961 agacttttat tctcttcatt agttttttcc tttcttgcca tatatcttat tatattgttc
    22021 ataattattt tttacttttt ctacatattt tgaagtttgc ttaaaaggta tatcatgtag
    22081 attttcgcca tctttactat actcattatt ttctaaccat tttttcacat tacctgaacc
    22141 accattatat gcagctatca ctaaatctaa tttaccaaat tctttgtata acttacttaa
    22201 ataccaacaa ccaagtctta tattagtctc tgggtcgaaa atatccacat tttttagatt
    22261 taattcctct gctccccaat ctctagttat gtctaatatt tgcattaatc cttttgcttc
    22321 tttttttgaa acagcagaac tattaaactt actttcggct ttaataacac tgtaaactat
    22381 attttcatct aaattaaatt ctttagaata cttttctaca tattctgaat attttttagg
    22441 atataaaaat ttatgtatta ctttgctttc cattaatagt gctccaaata agattataaa
    22501 aatagataga atcaatactt tcttactatt cacgccaatt ctccttcatg tactcaataa
    22561 atttatgtgc tttactttct aattcagtta ttgttcccga attatctatt atataatcac
    22621 catatttgga tttttcttcc tgactcatct gtgaatttat cctattaagt gcttcctgct
    22681 cactgcaatt atctctcttt tgtattctac taatttgtac ttcttgttta caagtaacta
    22741 ctagtagctt gtcaaccata tctaaaaaac cactttctac caatattgcc gcatctaata
    22801 ctactatatt ttcgcctttt tttctcaatt cttctatttc acttattatt ttttctctta
    22861 ttcgtggatg tgtcaaatta tttaacttct taagtttttc ttcatcacta aaaactattt
    22921 tcccaagagc ttttctattt agagttccat catcattttt tatactttgc ccaaaatgga
    22981 caaatacttt ttctaataac aacttatcct caaatatctt cctggatata atatcagcat
    23041 ccactataga aatgttaaga tttctaaata tatttgatag gctgcttttt ccacagccta
    23101 ttcctcctgt aagtcctaat atcaacataa aatcacctac tttgtctcat accaagaact
    23161 gcctacattt aaatctacat ctaagtgaac atctatattt acagcatttt ccatttcttc
    23221 ttttactatt ttcttaacta tatctatttc atcatcaatt gcttcaacta tcaattcgtc
    23281 gtgaacttgt agtataagtt ttgatttcaa attgttctct tccaattttt tatgaacatt
    23341 tatcatagca atttttatta tatctgctgc acttccttgt attggtgcat tcattgcaaa
    23401 tctctttcct ctatttcttt ccataaaatt gcttgacttt atttctggta tatatcttct
    23461 tctgtttaaa attgttacag catatccatc ttcactagcc ttttctactg ttgtgtccat
    23521 aaactctttt attttatggt atttagcaaa ataactttcg atatattctt tcgcctttgg
    23581 aacaggtata tttaaatcat cagctaagcc aaagtcactt ttaccatata taataccaaa
    23641 gtttactgct ttagctgcac ctctcaattc actagtgaca tcttccatag gaacattaaa
    23701 aacttgtgaa gctgttttag tatgaatatc ttcatgatgt ttaaatgctt ctatcatatt
    23761 ttcatcctga ctcatatgtg caagaactct tagctctact tgtgagtaat cggcatcaac
    23821 caacttacag ttatcatctg caataaacac ttttcttatt tttctaccca tttccatttt
    23881 tataggtata ttttgaaggt ttggttctgt agatgaaatt cttcctgtag tcgttatagt
    23941 ttgattaaat gatgaatgta ttctattact ttctggattt attatattta aaagtccttc
    24001 aacgtaagta gaatttaatt ttactatttg tctatactca gttatcttat ctattattgg
    24061 atgcttatct cttaattttt ctagaacatc tgcattagtt gaatatccag ttttagtttt
    24121 tttaattatt ggaagctcta atttttcaaa taatatgaca cccaattgtt ttggtgaatt
    24181 tatattgaat ggctctcctg ataattcata aatttccttt tctaatgtag ctattatttc
    24241 tttaaactct acactcaatt catccaatat acttttgtct acttttatac cttcatactc
    24301 catatgtcct aatacttcta ctaaaggcat ctccacatga taaaaaagac catccatact
    24361 catatccatt agacttcttt ccatcatagg aactgtttct ttaactgtgc atattatttg
    24421 acccatatac tcagctaaat cctcaaattc taaatcttca tagtttttag atttaacacc
    24481 ttttcctaat aattcttcct ttgatttaac cttttttgat aaatatttca tagctattgc
    24541 actacaatca tatgaagtag atgaagttga atctattaag tactcagata ttgttatatc
    24601 aaaatacata ttttctaagt ttatattata tggttttaaa gatatataat catcttttaa
    24661 attatatcca tactttttga tttcattatt tgaaaatata ctatctaact ttgacacttc
    24721 atttgaatct acataatata ttttttctcc atctacactt ataaaaatat atattatatt
    24781 tttttcaagt atattacctt gtcctctaac agtttttaaa ataacttgtc ctttttcatt
    24841 tacttcatct ataaattttt ctatatcttt taattttaat atttctattt tttcttctac
    24901 ttgttctgta tcatttgaat cgaccaaatc aactagcctt gacaaaaggc ttgtaaatcc
    24961 aaaatatcta aatttatcta aaagttcatc tttatcataa tcgccaaaaa tcatactttc
    25021 taaatctact tctattggca catctcttat aattgttgca agtcttttac tttgaatagc
    25081 tatttcttta ttttcctcta ttttcttttt tacactgcct ttaagtttat ctgtattctc
    25141 taataagttc tctatactag aaaactcttt tataagcttt atacctgttt tctcaccgat
    25201 tccaggtact ccaggtatat tatccgactt atctcccata agacctttta aatcaataaa
    25261 ttgtgttgga gtcatctcgt atttttcaat tacagaatta aagttgtatt cttcaacttc
    25321 tccaactcct ttttttgtta taagagtagt tgtattatct gaagcaagtt gaatagcatc
    25381 tttatctcca gttactatgt ataccttata gccatcatct tctgcttttt tagaaacagt
    25441 tcctataata tcatctgcct catagccatc tatttctaat ctatttattt taaatttatc
    25501 taaaaggtct tttagtggct gtaattgttc tgccaattca tctggcattc cttttcttcc
    25561 agccttatat tctttatact ctaaatgtct aaatgtaggt gcttttctgt caaaagctac
    25621 acttatatgt gttggtttat atatatctat cattttaaat agcattgttg taaacccata
    25681 tatcgcatta gtctttaacc cttctttatt gttcatttct ggcagagcat aaaaagctct
    25741 gtttattatt gaatttccat ctattataat taattttttt tccaaattat atcactccta
    25801 aaaattatct atttttgtaa catatatatt atataccaat atagtgttat ttaatattcc
    25861 cataataata atagtatatt aaaattaaat atgttgcaaa caatatattt ttttgctata
    25921 ataatataca tgacatgccc gagtggtgga attggtaaat acaatggatt caaaatcaac
    25981 tgtgttgatt cataagtttt tatgatgttt tacagtttga tgagtatttt gtattcaata
    26041 ttcctcttgc atattcttgg aaaatctggt aggatataca agtagttcgg atttaaaata
    26101 taagccgctg tggcggaact ggcatacgca tacgactcaa aatcgtacgg gaaaccatat
    26161 gggttcgagt cccatcagcg gcaccaataa aaaaaactct ttagtatata ctaaagagtt
    26221 tttttattgt aatattcttt catctttatt tctagtcttg tataagtata aataccatag
    26281 tcataacttc ttgaactcct aaaatatatc taaacaaagt aggatttttt atgtattcac
    26341 ttaataaatc catacttctt atcatattga ttttttccca ctctctaaaa cttttccttc
    26401 tctaaaaata tttataacca atcatcatta ttcagtataa tgattctata ttattttaaa
    26461 gtatttgtca tttaaaacaa acttgtgata aaagcatatc tgaaagtatt ctgattctaa
    26521 tacttaataa agagttcttt ttacagttat agatataaac aacttctatc ttaatagact
    26581 gtatagaagc acaatcaaat gcaaacacat atagttaagt aatatgtcct tgatttttct
    26641 ttattatttt attgtaatag ctttattaat tcattataat attacatcat ttaaaaaaaa
    26701 ttatatgaaa agtcaaaatt tacatataga ttaattaaac ttgtaaaaac acatataaat
    26761 tcgtacaatt aagtacttaa aacttgaaaa tagttacttt gctatatatt ttagataaag
    26821 atataattta tttggttaag aaaagaagtg gtaaatataa aaataaggag tctaatatga
    26881 tattcagtag tttaatattt ttatttttat tctttccagt tacactcatt tgctattact
    26941 tggcaaatcc taagtataaa aatgttatgt tacttctagc aagtattgcc ttctatacat
    27001 ggggagaacc aaaatatatt tttttgatga tactatcaat aatagtaaac tatatatttg
    27061 gtattaaagt ttctggcagt aaaaataaaa aaatgtgggt tgtgctttct gttatttaca
    27121 atatatctat tcttgctgta tttaagtata gcaatttttt tatagataat attaattttt
    27181 tattcaatac agatataact atacctgagg ttaaattacc tttaggtatc tcatttttca
    27241 cattccaaat aatgagctat gtaatagatg tatatagaaa tgatgctaaa gttcaaagaa
    27301 aattagctaa tttagcattg tacataagtt tattccctca acttgtagca ggtcctattg
    27361 ttagatatca gactgttgca gatagcattg agtatagaga acacaattgg gacaaattta
    27421 ctcaaggtgt aaacagattt gttgttggtc ttggaaaaaa agtaattcta gcaaatcaat
    27481 taggtgtaat agcagatggt gtatttaata agtcagctgg agatttatca agatttgagg
    27541 tatggctagg tatagtttgc tatacacttc aaatattcta tgactttagt ggctatagtg
    27601 atatggccat tggtttaggt aaaatgtttg gatttgagtt tttagaaaat ttcaactatc
    27661 catatatctc tcaaacagta tctgagtttt ggagaagatg gcatatatct ttaggtactt
    27721 ggtttaaaga ctatgtatac ttcccattag gtggaaatag agtctctaag gcaaaactat
    27781 atagaaatct atttgtagta tggtttttaa ccggcatgtg gcatggaact agttggactt
    27841 ttattatatt gggtctatat tatggaatat taatatcagt agaaaaagca ttcttagaaa
    27901 agtttctata taagatgcca agaattgtaa gacacatata tctacttctt atagtcatga
    27961 ttggatgggt attttttaga gttgatgatt taccagcagc tattaaattt ataggtatta
    28021 tgtttggtgc tggaaattat ccggttatca gtaatgcatt caatgtgttt ataattgaat
    28081 attggtatgt aattgttggg gcaatccttc tatcaactcc tattgtaaaa gttttaaaat
    28141 cattattaat taagaaaaat aaaaatacat taaatagcac attatatcaa gttactaata
    28201 tggttttcat tgtttcttgt atgtttattg taataataat gttaagtagc tcaacttata
    28261 atccattctt atattttaga ttctaattgg gaggtataaa catgagtaaa aaaaaaccaa
    28321 ggattgttgc catttcattt ttaattatag tatttggatt tttcatacta aacttcacac
    28381 ttccaaagaa aaaattaagt ataactgaaa atagaaatct tgcaaaattt ccgactgtag
    28441 aaaatttaaa aaaaggtgac tttatagcca agtttgaaga atattttaat gaccattttg
    28501 cattcagaga tagaatgata tcaataaata caaaggctca agctagttta aataaaacta
    28561 aggtaggtaa ctattatcta ggaaaagata actggatatt aggtatgttt ccagaaattt
    28621 tagaccaaaa acaattaagt cgatacgaaa agtccattaa ttatttatct aatctaagta
    28681 aagatatggg aaaaaatgtg tattttacaa tgatgccaca taaaactaat atgttaaaac
    28741 atttgtatcc taaatatgtt gataatatgg ataatataga cataaattta aagaatttta
    28801 aaaatagatt aaatccagat ttaattaaat atatagatat ggatgagtat tttttaagta
    28861 actattcaga taaagaaaga gaaaatttat attttaaaac tgaccatcat tggactggat
    28921 atggagcatt tgaaggcttt aaaatgatgg catcaaatat ggacttaggt ctatctaagc
    28981 aagatttaga caagcatttt agtagctata aaaagtctat aattactgat aagaaattta
    29041 taggaagtta taatcaaaat atagatatgc taattaaaga aaatgaatat gtagtttata
    29101 tgacaaaaga taaccaagat tatagctttg atataaatgg agaagaaaaa gaagaaaaag
    29161 aggtttatgc aactgaaaga gataataaag agtgggatta tggtggtgca tacattagag
    29221 gagctaaaac taatatatta actataaaaa accctaaagc attagtgaat aaaaaaatac
    29281 taatatttag agattcatat caggctccta caacactttt atttgctgat atgtttaaag
    29341 aagtagagat agcagaccct agaaatataa actcaatata taaaacatac aaagagataa
    29401 tcgaggaaag taatgcagat attgtaatgt ttatgtataa tagttctggg tttgattcaa
    29461 tgatagattc aatgatagat aaaggaataa agtagtaaat aaaaagatta aacaaaagta
    29521 ttatctaact aaagatggat ggctattagg caactttgcc aaattattat ctgattatca
    29581 tataaaatct tactccaaag ccattaataa cttgagtaag ttaaatagaa aaaaagattt
    29641 ttactttgtt tcacttcctc ataagacaaa tatgcttaag aacttgtatc caagtggtat
    29701 aaaaggtaaa aataatatag atattaacaa agaaaactta gaaaagcagt taaaagacat
    29761 aaatttctta gatatagatg attactttta taaaagtttt accgaaaaag aaagagaaaa
    29821 cttcttttta aaaacagacc atcacggaaa ttcattagga gcatttgaag aatataagtt
    29881 tattcttgat aaattagacc ttagcttatc taaatcagaa atagatagtc atttaaaaag
    29941 atacagtact cgatatgtaa atgactgcga atgtagtttt atttatgtat aatagctttg
    30001 gatttgctgg aatgatacaa gaaatgattg acaagggaat agcataacaa tttgaatcaa
    30061 ctccttttta tacaaaaaat agttctcaat aattcctaat ctatatgata atttagtaca
    30121 tctctttaac aataaaaata aaatcacata aattcttata ccatcactta tctttatgat
    30181 aaattggttg agaaaaaatt ttataattta tatgatatgt aatcttaatt aattctttac
    30241 tttttaattc tttatctact gcaacctttg ataaaagaaa ccatccaaga ctattaatat
    30301 gaatttatat aaaaattact ttcaaataaa gtactgcaat atttttattt aaagtatcta
    30361 taatctttaa ccacaaaaag ccacaaaata caatgtatac ctatggcttt ttataaattt
    30421 acataattgt atataaagat atactctaaa actgtcttaa gaatctaaag tttaaaaata
    30481 caatattttt attgtgaatt taaacttttt attacaactt tttatctaat ttattgtata
    30541 caacaacact acaaataaat tgaaataaaa taagcaatcc aagaacaact aaaacccact
    30601 tgcttattac cattacagaa aatacactca atatagaaac aataaatata agaattacaa
    30661 gaacatagga catgatttga ttagtgatac gtccagcttt atcacgtaac ataattcttc
    30721 tttcatcatt catctctatc ctttggtttt ttatcttttc ttcataatct aatgctcttt
    30781 caggcttact ccaatacata tattgaagaa tcatacatac acctggacct aaagcagctc
    30841 cagataatcc ccaaataaaa gcctcaaatg aaaactcagt aaacgtagcc aataaaacaa
    30901 gtaaaattcc acaaataaaa tatactattc caagatataa catattcttt ttcattttta
    30961 ctcctcctca taaataaata tttcttcaat actcctatca aagtatctag ctatcttaaa
    31021 ggctaagatg atagaaggat tatacctacc attttctaat gaacctatag tttgtcttga
    31081 tacttgcaaa acagaagcaa gttcttcttg ttttatgcct ctttcttttc ttatttcttc
    31141 taatctattt ttcacatcta tcccccttta atatctcaac tacggaaagt ctactttcca
    31201 taattataca actatattcc atttatgtaa agcttacttt ccatatttac aaaaaaataa
    31261 cagactattt tcagactgta attttagaat ttcatgttgt ttattaattt taatgttatt
    31321 tattaattcc atattattta ttaactttaa cttggtttct gaattggaat ccaaacttct
    31381 gtagtataat caggagaatt gatatcgcca ttgggataaa gttcaatatc tggtaaatta
    31441 gaatacacat acccagaact tggtaaccac tcagtaataa ttcttttttg caactcctgt
    31501 attgccagag catttggcat tggagaaaca cattcaaata ctgcccatgt acctgatggt
    31561 attaagtatt catgagtatc ttctaatata ggcttatctg ttggtgcagc aatataataa
    31621 tctaagccct tactatttgt acacacacta actccaagca atccacaagg ttcgttattt
    31681 attaactctg aaagtttatc aattgttcca ttttttatgc atttagccca taacttagga
    31741 actttctcaa aacactcttc tacaatcatg ttcatacttt cttttatacc aacaatacga
    31801 aatcctttct tttctacaat acgatattgc atttctacat ttccttttac ataaatgctt
    31861 aatgtcatct taggatatgc ttttaaaaca acgccttttg ctctagctgc agatggagaa
    31921 atgttatgta tattttgaaa agctctatta aaagatgttg gtgaatcgta accatacttc
    31981 tcagatagtt ctaaaatttt gatgttactg ttctgcagtt caaatgcagc cttagtcata
    32041 cgtcttcgtc taatatattc agatactggt acttccgcaa tatatgaaaa cattctttga
    32101 aaatgaaaag ttgagcaaca tgcaattttt gctgcttctg cataatctat tccactttca
    32161 agattttctt caatataagt aatagcttga tttaatttat ttatccattc catttaatat
    32221 tacctccaaa caaattatac agtttaataa aataatatac ctctctattc atgcacaaaa
    32281 ttgtaagcat ttaaattcat aagcattttc taatccatca atacaataaa tgtaaaaatt
    32341 atattataca ataaaaaagt tcaataacat tatataatat tattgaactt cttataaaaa
    32401 ttaatttaag tgtagttaaa ttaagtatta cactatctta tatattacaa tatccaacta
    32461 tcttgtagtc ttctggcact ttataatctt tatctggagt acctaaattc cattttaact
    32521 caaacatagt tgcacttatt attgttgcaa aataaagcat aactatacca atatctattt
    32581 tttcttcata taaattagta tggccatctt ttctaacagc taaaattaca gtgcctccat
    32641 caactataaa tctccatggt tgtctattta atgtagatgg agctaatctt gcataatgaa
    32701 atgcatcaag aagtgctctt tcttctaaaa cttctatgct tgcaacattt ccccattcgt
    32761 tcatataaac aacatcttct acacctagtc tagaagaacc cttatctgtt acaacttgca
    32821 tatcagattt tgaataattt tgcccagttt ttgtagcatt taacacttta gcattattta
    32881 cattttctcc atatccaata gctataattc ctgttacctc tttatctgaa tcaattccaa
    32941 gtttttcttt tacttttttt ccatcttcaa aagtaaccca acaagaacca attcctagtt
    33001 ctgttacttt aaagattata ttttctccta tgtaccccga attttcaata taattatctt
    33061 ttacatcaga taatattata atataacttg gagaatctat catatttcct ttgtatcctg
    33121 ctactttttc taaattatta aagacttcac ttctattcat aattcttata tcaacatcta
    33181 tttctggaac tagtttctta caagattctg agtatttctt aatttcacta agatattcct
    33241 ctttaatatc tgtgttctta tattctctaa cagactttct atttaatata attgttttat
    33301 aatccataat tttatcctcc ccaatctagt cttgttttaa ttatataccc attaatatat
    33361 tattttactc atttatttaa taaataatga ttacaatttt gtaattatta tttatcaata
    33421 acattccact tttacattaa taatgaccag ttgtgtaaaa tccattgcca accaccaaat
    33481 aaaaacatat tataaaggta atataaaata acaaacaaat cttaacaaaa aattaaactt
    33541 tttttacacg aaagaaggct tcccgttgcc ttctttattt ttataaaaaa ataagctgtt
    33601 tctaaaatga tttcacattt ttgaaacagc caagttattc tataatgaca actataattt
    33661 gaggatgtga gggagagttg tcattataaa tttagggtaa ttatattatg ttttaagcta
    33721 gttctttcta tatttatata atacatttta tatatgaatt ttatatgaga ttggtataaa
    33781 tgatatatga aattacatat gattacatta tattaaattt tattattaca ttttccacaa
    33841 caatactcat atgtctccac attattcgga atatttacaa acacaaattc tttacagttc
    33901 tcacaatact ttaatataga tatagttcca caagtgtaac attcaaatat tacacaatcc
    33961 tcatgttcct ctactatttc aaacttttta ctatgttcac cacactctgg gcacttgtga
    34021 ttatttacat tcataaatac tcctccttat tggtatagtt acatgtcaaa aacttaatta
    34081 atgtattcta tattacttat tatatattct ataataaatc atattctaat ataataaaac
    34141 aaattaaata aaaaaaaacc ataaatttta aatttatgga ttaattcttg taatgataac
    34201 tctatttgag gatgtgaggg agggttatca ttacaatagg gtattatatt atggttttta
    34261 agctagtctt tctacacttc atataataca tatgctatat gaattttata tgatttttac
    34321 ataaatattt tatgaaattt aaatttttta gtcattttga aatttgtaaa aagttctcta
    34381 aaaatccaca gcataataaa tagccaaaat ttaagtaaaa catcataaaa aaatttgtcc
    34441 aataatttta acaagtcacc tcacctcatt ataattattg acacaaactt aaataaacaa
    34501 tctataaacc ttctacaaaa tttaaatttt cctcttattg atacacaatc tctagtttgt
    34561 caattttatt ttgcatctct aagaaaattt cttcaacaga attgttaaat atattgccta
    34621 aatagactct tttatctttt tgaaatgaat aagattttac attaccatta gaagaaatat
    34681 ataaatgact ccactctgct ccatattttg aaaatctctc ctttgtatct ttaaacctca
    34741 tatcttctat ttttattgct ttttctctgt ctcttcttac tacagtatga ccccttttta
    34801 ttaaactagt tatttttata tcttctttta attttatacg tgtatcatca tttcttgaat
    34861 atactttcca tatcacttta tgacgataat tatctaattc ctttattaaa cttctatcaa
    34921 tagtaaagct atctttagtt accacaatta aatcaaaatg gtctaacata tattgaaata
    34981 catctctcat acctttattt tttaaaatat cctcaccaat aacctctata aatctacagc
    35041 ctttctcata catacacttt aataaacttg tgatttcaga aaaataatta tttgtattat
    35101 tatctgcatc tatttctaga gtaaagtaca aaggagtaat gtatgatttg tttcctgtta
    35161 tctcaaatac acttgatact gtttgtatat tatcttcaaa ggttaagtat acattctcat
    35221 aaaacataaa aaattccata actttttctc tcatattagg gatattccta tatctattgg
    35281 ataatatatt tattatgtcg tcagtagtat ttttcccatc acattcagcc agtacaacaa
    35341 tttgcttcca acttaagata tccacatttc cgatactttt tttatatcca tcttcttcat
    35401 cataaatagg accaattata atgttttctt ttggccacag atgaacccta gttccatctt
    35461 ttaatttcgg atatttattt cttacagact cataaaatct acttgtatca cccattattc
    35521 tttactaccc cccatatttt aaatattcta tgctaaaaat agcactttat ttttattaat
    35581 tcaacaatta ttcaaaattg gttattaaat atgtttaaat ggatttaaca tcaaccttat
    35641 ttatatgtta ttgatgttca tatatgttat ataaaagctt atttcttaga cttcaatgta
    35701 attttaacta tttctggctt acaattaagc ctaattaaaa cttttgagcc ccctaagcct
    35761 ttatttaaaa tcatagtgct attatctttt tcatatctgc cccaatcata cttaggaaaa
    35821 aactctctat ttggagataa cacacctcca acaaaaggca ttcttattat acctccatga
    35881 acatgtcctg ctaaaaccaa atctgctccc cattttgcat atttttcaaa gaaaaatggc
    35941 gtatgtgcta gaagaatatt atactcattt tcatttactt tacctagatt actttctaca
    36001 aaattgtcac ttagttctaa ctttttatta ttagcaaaaa aatatggata acattccaat
    36061 gggacaatta aaccatataa attaagatga ctattccctt tatatattct tacagattca
    36121 ttttccagat ttattatttt ctttgaatat agttccttaa aataatcttt atatagatgt
    36181 ttatattttt ttattaaaga tttttgttca tgatttccaa ttatatggta tacttcatat
    36241 ttttcaacca aattatctat taaatctaat gcaacttgaa aatttttatt ttctccatct
    36301 actaaatctc ctgttataaa tataacatct ggatttaatg atttaatttt atccatcaac
    36361 accttattgt ttttatcaaa taccttatta tgtaaatcgc tcacttgaac tattttaaag
    36421 ttatcaaatt ccactggaat ttttttgttg aaaatagtat attctgtcgt ttctatatgc
    36481 tgagtttcgt acttatacag aatatacaat aatattaata ctagtactgt cattgaaacc
    36541 tttataatca tattctcttc ctctttattc tattttatta atattattat aatgctttta
    36601 tgtttttttt aaaacaataa catcatcaca tacatataaa tcttttcgac attataaaaa
    36661 taagttatcc ttttctaaaa ttttcattta ttttagaaaa gaataactat aaaattatct
    36721 ataaacctct atattgtgaa atatagtttt tcctttattt aaatttttat atatatgctc
    36781 taaagttcca tcaaacctaa tagaatgccc agactctaaa atataagttt tcccatccac
    36841 attaaggtca agtttacctt ctattactag cacatactct tccaaaacat gactatgtgt
    36901 agatgaaaca tgagtacagt tttcttcaag ctcaattata aaaagttcaa accttctttg
    36961 agggctaaaa gggtatattg gatatagttt catattccct tgttgctcaa caataggctc
    37021 tacttcttct ttttttataa cctcataagt atttgtattt tcatttaata gtgtagtaaa
    37081 atttatttta aatccagatg aaattttcca taaggtggat actgttggac ttgattcacc
    37141 tctctcaatc tgtccaagca ttgctttact gacacctgtc atctttgaag ctttatctaa
    37201 acttatgccc atttcttgtc tcaataatct taaattttct cccacattag gaaatttatt
    37261 atccatgtta aaatcctcct gatagctatt tgtattctat gatttttacc cctgtaagtt
    37321 ctactatctc ttttaatttt aacatatgat tgttacaaat ttcatcaaat tcatatatat
    37381 aatcttcaag tcctaatgcc ttatatttat cgattccaaa tttatgatat ggaagtaatt
    37441 ccatctttcc ttctggaaca ttctgcttca caaataatgc agtatttctt atgttttctt
    37501 cactatcatt tactccataa ataattggca ctcttataac catagattta ttcaattttg
    37561 aaagtctaca tatattatct aatataattt tattacttac tcctgtatat tctttatgaa
    37621 tattatcatc catggatttt atatctacaa atatgtggtc tattttatca aatacatcat
    37681 ttaccttatt ccaatcaaaa tatccacaag tttcaattgc aatatttatg cctttattat
    37741 aaaaaatatc aactaattct cttaaaaaat caatctgaag tgtaggctca cctccagaaa
    37801 aagttatccc tccattagat tcaaagaaaa atataaaatc tttttcaact tcctttacta
    37861 tttcttcaac actcatttct tcggtcatta tacaaatagc atctttaaga catgctttga
    37921 tacactctcc acataaatca catttattat ttatttgtgt tctatcaaac agtgaactta
    37981 tattttgagg acaaacatct acacatctat tacaagatat acatttatct ttcataacac
    38041 ctaatttcac tatattactc catgaatctg gattggaaca ccacttacat tttaatttac
    38101 atccttctaa aaatatagta gtccttattc catctccatc atttactgaa aaatgttgca
    38161 cttttacaac tctacctttt ttactcataa tctacctcat aattaaaaat tactatgtga
    38221 atttctagct attatagcat cttgcatttc tcttgataaa ttaacaaatt gagtactata
    38281 tccagcaact ctcactaata aatctttata ttcttctgga ttttcttgag cttttaatag
    38341 agtatcattt gatatcgtat taaattgtac atgaaatcct cctaaggcaa aatatgattt
    38401 aaccatagat gttagatttc taagtcctct ttctgtttcc accaagtctt gattcagtct
    38461 taaatttaga agtgttccac cagtatgaac atcatgattt atttttgatg cagatttcat
    38521 agctgctagt ggacttgttg tatcagtacc aacaaatgga gaaactccct ctgtcaaagg
    38581 ttttgttgct tttcttccgc aaggagttgc acctacgatt tttccagttg gtacatagtt
    38641 tgatataccc ataaatgcac tattaaattt agaaccaaaa atatccttgt attttctagt
    38701 ttcagtataa taaaaatcag aaacttcaat tgctaatgaa tctacataat cattatcatt
    38761 tccatactta ggaacatcta acgcaagttt tcttaactct tcataacctt cccaatcatt
    38821 atttagtgct ttggttaatt cttccaatgt agtaacctta tcttcaaata ctaatttttt
    38881 tatagctgcc aatgaatttg aaactacacc taatcctata ccagttaata caggtcctat
    38941 attatatttt gctcctcctt tgcttaaatc gaccccctta tctaaacatc catctacaca
    39001 agtagataaa aatggtcttg gtaccatttc tttatgaatt tgttgagcaa cagtagttcc
    39061 tataacagaa ttttttacaa gatatgctaa ttgatttaaa aactcatcct taagttcttc
    39121 aaaagaagta aattctgtga tattttttgt atctagaccc attttttctc cagttaacct
    39181 actcttacct tcattcatag catattctaa agctgctgca aaattgatat ttacggctga
    39241 agtccactgt cctgtttttc taaaatgagg aactacacat ccacagttac tccaatctct
    39301 agcgtcttct gcatcatatc catcttgtag taacatttgt gcccctgcat tatcgctgtg
    39361 tatagcaggg aagccagttc cttgttttac taatttagca actgccatga caaaattatc
    39421 tggtgctcct tgactcaccc taacacttaa tcctggctgg tgagttttta cactttctgt
    39481 agcctttaaa cacatatatg agatatcatt cgtcccatca gtaccatttc tctttttacc
    39541 accaacagta agattttgaa attgattata tcctgcaaaa taatttgccg tatttgctga
    39601 tatagtccat acccattctg ataacttaat ccaaagtgct tctatcaatt cttgagcttc
    39661 tgattcagtt attttgcctt ctctagcgtc gttttcataa tacggataca tatattgatc
    39721 aaatctacct aaatttaatg ccaatggatt ttctgataga ataccaccta gttgaacaaa
    39781 ccatacaaat tgtattgctt cataaaaatt agttggaggg tttgctggta cattcataca
    39841 tatctctgaa atttttataa gttctaactt tctctttttg tctgtttctt gttcactcat
    39901 ctcttttgca agatttgaat atctttgtgc caatgtcata ataccctgtg caactataga
    39961 aacagactta taaaagtcta ttttttcaat attttctgaa atcgatatat ctaattcttt
    40021 aattttttta tctgcatctt ccttaatccc tttatatcct tttacaaaca gtatatcttg
    40081 ataatctggt gttacctcac caactgcctg tctccactta gaatcattat ctattattcc
    40141 tgtatctact aataccttag ctgtatcttc tgggattctt gctaaaaaaa cttcttccaa
    40201 agattttccc ttccaatatg gaaatatttc ttttcttata aattctattt gttcttttga
    40261 tattacatat gggtcttgag ttctttcatc aaaagtatcc atttctttgt ctacccattg
    40321 ccaagaaaac tctggtgtta atatccctgt tcttctaaat tttcctgctg tacctactat
    40381 taattcattt tcaaagattt ttataggaag ctctttacat gtctccaaaa aagctttagc
    40441 tcttcttata cagatagctt ctccctcagt ttttttatgt gattcagtaa aaatcctagc
    40501 tctttcacaa catatcatag gtttagaatt aatatattct tgtctaaatg cttctattct
    40561 attagtttga ctcatttaaa atcccccttt ggataaatat atacttatat ctttgtatgt
    40621 tataacatac tttaattata ttatactgtc ctttagcata aaatggaagc aaaataattt
    40681 attttttcat ctaccttgaa cacctatata ttaatacttt ttttgctaaa tttgtaaaat
    40741 ctaaaaaatt tgtctatgat atatttttta tattgttata gttatttcta gtatactaac
    40801 gatttgaaat atcaaattta tataataggc ttagttttaa aactttagtt gattgtttaa
    40861 ttttgttaac ttcaacatgt atatctttca tataaggaag ctctacaaca cctactccct
    40921 tattaaagtc ttcttcaacc atagaaaaaa tttctttctc agttaagttc gtattagtca
    40981 atgacttaaa taaattatat ataaatttaa tatatttatc actctcagat aacttctcat
    41041 cttctggata ctctttagca cagagaatat ctattgttac ttttccttct ttatcatctt
    41101 cagatacaga atagaataaa cttctaatat tttcttcagg tggtttttca acactattta
    41161 tagacactac aaagctacca tctgattttg aattagtaat actataacca ctgtctatta
    41221 tggctttcga aaatctttga gataaatctt tttttacttc ttctctacct ttcacatcat
    41281 tcatcttttg cgagtaatta ttaaataact caatagattc atctttttta tcttcaactg
    41341 attcttttaa tgaatcttta ctaatttgtt ttgaactact taagttctta tcagatacag
    41401 gagacttaca tcctaccatc aatgctaaac taagatttaa agccacaact attgccactt
    41461 tatttatatt tctctttact ttcatacaca atcctcccat cagactaacc tctctaaaac
    41521 attattcatt gtattagaat atgtttatat tctctttata ttaaaaattt attactcaac
    41581 acatatcacc ttcctatcta attcttatag attactatta gtaaaattat aaattcctta
    41641 atacaaaaat aaggatgtta tcttttgtta ttaaaagata acatccttgt ttttttgtat
    41701 caatacatat gtcccaacta gtaacttaat cgctccaact gtcagtatct tggtccaatg
    41761 gtatgtatat atttagtata aatttatcca tactattttc aaatgataat tctccattat
    41821 atttttgtaa agaagattta atactttgaa tacctattcc gtgcacaaac ttatccattt
    41881 tgtttgttaa caatatattt ttattgaaag taatcttatt tagtttacta ttttcacatt
    41941 taagtacaaa aaatgacttt gttatagttc ctttaattct aatatattta ttaatatttt
    42001 catccaatat tttatcacat gcttctattg cattatccaa aacattagag aatatacttg
    42061 aaacatctat aggttttaca aaatttactt ttgaaaaatt tatagagcaa ataaatttaa
    42121 ttcccttttt taaacatatt tcactctttt cattgattat aatgtctaat atcatatttc
    42181 ctgtattata aatatattta aatgctttta tttcatcttt taaactgtta acatattcgt
    42241 caatttcttt gctattattt ctaagcttgt ctatacaata aatgtgatta tttatatcat
    42301 gatatagttt cttaactcgc atatgagatt cttgaatact taaataatgt gcatattgca
    42361 tatctaactt attttttatt aattcagctt gagattttaa tctattttct tttattattt
    42421 ctttaaacaa tagtataaga aatatactac tagctcctac caatttggga ttcacaaaat
    42481 tatcaaaata gtatccttct gaatgtaatg acccaaaccg ttctaatttt tttaatacat
    42541 aatttacaag catattcatt gtattagcta ataaggcaaa taataaatat acataatttc
    42601 tcttatcttg actaattaat tttatagact tcctaaaaaa gctaaatata agatatataa
    42661 gaataattat taaattacta tatatctgaa tttttataga ttctaagtga ggtattaata
    42721 aggattcttt agatgaaata acattacctg taattgcaaa aaatagtatt ctatatataa
    42781 cagcatccaa acattcataa tatacactaa atagataaaa gaaaacagaa tacttaatat
    42841 aacctatctt ataaaaataa ttatagtatc ctccccaagc aataaatgct aatatataaa
    42901 ataaatagtt tatattacta aagtaatttc ctccattaaa taatctactt atctgaacta
    42961 taatgtaaat aagaaacaaa acaatataaa atactatttt ttcatttttt ttactatcct
    43021 ttttataaat taaaattccg ttcattatag aaaactgtat aaatatagat aaaactacta
    43081 atagctcagt cataaaattt agcatattgt atcaccaaga aattttaaaa atttctcctt
    43141 aacttcttta tatctatatc tactaatagg tacctcttct ccactttcca atatcacagt
    43201 attaggcttt atattttcaa catatcttaa atttactata aaacttttat ggcatcttat
    43261 aaatttatat ggatttaatt ctttttctat tttacttaaa ctatatttta tatcaaaatt
    43321 cttatttatt gtgtgtatta gcatatcttt tttttgaact tctatatatt ttatttcatt
    43381 taaataaatc ttatatgtat tagacttatt ttttattgta atatgacttt tcttatttat
    43441 ttcaatatct ttgatacaag ttaacacatg tttttttaga tcttctagct ctattggctt
    43501 tagtaaatac ctataagctc ttacctcata tccttcttgc acataatcta ttaatgaagt
    43561 tataaatatt atttccattt cattatctac ttctctaatc tttcttgcag tatccattcc
    43621 atttagtttt tccattttta tatcaagaag taatatatct atatctttta aattactttc
    43681 caatagatct tctccagata gatacttttg tatctcatat tctatattta tatcttctaa
    43741 tatattctca aggtaatctt ctattaaaag ttgtgtttct ttttcatctt cacaaactgc
    43801 tatcttaatc ataaaatgcc tccttacata atttctagtt aatacaatta aaatttttat
    43861 aatatattta aagataattt tgtatatctg atactttagg ataacatttt caaattattt
    43921 ttatcaatac tcaatcctat acaactgtta ttttgatatc tcaatttaaa gacagaaatt
    43981 actataatta aaaacagtac ttactgataa aagaagatgt cttaaaatat caaaaaaata
    44041 attttaggac accttctttt atttaaactt tttaagcctt aaataataca ttaatctcct
    44101 atataattgt actgcatata aaatcaacaa tataatctgt agtacaatag cagttacaga
    44161 tgctccacaa ccactatata aatttcttat ttcaatattc aataaatata taaatattac
    44221 tactaacata aagaatataa acatattcat aaataaaatt gactttactt tatttttttt
    44281 atcagaatca ctatataatt taggaggtat gtctttgtat tctttcgacc atatatacca
    44341 tgcagatgta cccataaaac tgccaatact cctaaacaca agattccagc catttgattt
    44401 atgttcatca aaataattat tctctgcatc atagtagtaa tcaacagtgt attttatttt
    44461 tcttggactt tccttatgaa aataaaatat tcctccaaat ttatttatcc tatataaatt
    44521 atatccttta gcttcaatat cttctaacca tttctctatc ttatcaggtg aactatacca
    44581 gtcaaatttg aattttattt tcttttccat aacactcatt ctccaaaatg atatttttaa
    44641 atagcttaaa aatctattta aataattata tcattttgat actaatttta ggttacaatc
    44701 agatatttaa aaagctattt aaatagtaat aaactaatgg ccataacgcc cattcctgca
    44761 atcaatccat aaatggataa atgatgttcc ccgtactctc tagctgatgg taataattca
    44821 tctagtgata tgaataccat aattcctcct acaattgcaa atacaattcc cagagttata
    44881 tcattcaaaa aatttctaag aagtacatat ccaatgatcg ctcctaaagg ctcagacata
    44941 ccagataaaa aggaataata aaaagctttc tttttatctc ctgtggcata ataaactggt
    45001 acagatacag atataccttc tggtatattg tgtattgcaa ttgcaattgc tatcggtata
    45061 gctattgtaa catcatcaag agctgatata aatgtagcta atccttcagg aaaattgtga
    45121 attgctatag ccattgctgt aaatacacct gttctcaaaa gagacttatt ttttttgata
    45181 ttcttttctt ctatattctc cattttcttt atttcatgtg gattttcttc ttgtggaatt
    45241 agcttatcaa taacagcaat gatagccata cctataaaaa aagctattac agttgaccaa
    45301 cctcccaatt tttctcccat agattttgtc aaagcatctt gtgctttagg aaaaatttct
    45361 atcatagaaa cataaatcat aacaccagca gaaaatccta agcttataga aagaaacttc
    45421 gtatttgttt ttttagcaaa aaatgctata caacttccta taccagtaga taaaccagcc
    45481 aataatgtta tcaaaaatgc aaatattaca ttattcaaat aaatcacctt ctttttaatt
    45541 gatattgatt atcatttcat ttatgctatc atatatattt tatatagtca atatttatca
    45601 aaaatataca caataaaaaa gcagagatat aaccttagtc tctgcttttt cttcatagta
    45661 tattaaaatt atttattcaa gaaatccact gcatattgca catataaagc agttccaatt
    45721 tctagggcat cttcatctat attaaaacat ccatgatgat gaggataaca agcacctttt
    45781 gattcatttc tagctcctac aaatgctagt gcaccaggag ctatattcat aaactcagct
    45841 aaatcttctg ctcctgttac tttttcaaat agtgttatac agtcttctcc aaatagctta
    45901 attgctgctt ctgtagctat ctttgaacat tctttgtcat ttataactgc tggtgttaaa
    45961 taaccatatt ctaattcagc ttctgctcta tatgctcctg ctgtagattt tgctatacgt
    46021 tctaaaattc caggaatttt ctttctaagc tctggattaa atagtcttat tgtaccttct
    46081 aagacagctt cactcgcaat tacgttaaat ctagttccag aatttaaaac tccaacactc
    46141 acaaccaatg gttccagagg acttacttct ctactaacca tagattgcaa attcataact
    46201 attgctgaac ttgcaagcac tgcatcaact ccttgatgag gaagtgaacc atgaccacct
    46261 ctacctttta cagtaatttt aaagaaatca gcagaagcca ttcttggtcc ttcttctact
    46321 gatatagttc cagattctac atcagtccaa agatgtattc caaacacact atctacacct
    46381 tccatagcac catcttgaat catagctctt gcacctttgc caacttcttc tcctggctga
    46441 aaaaataatt ttacagttcc attgatacta tctttaatat catttaacac ttttgctgcc
    46501 cctagaagca ttgatgtgtg accatcatga ccacaagcat gcataagtcc ttcattttta
    46561 gatttgtatt ctacttcagt acattctaca acttgtaagg catccatatc acctctaagt
    46621 gctacagtct ttcctgaatt agcccctttt atcgttgcaa ttacacctgt acctccagct
    46681 gatacatatg gtattcccat tttatcaagt tcttctttaa ccctctttga tgttctaact
    46741 tcttccatac ttttctcagg attttcatga aattcccttc taagcttaat tacatagtct
    46801 ttataactct ttgttatttc tttaatatcc attacagacc tccaagtagt acacattctt
    46861 cttatttaaa atatttttta ttagttaaat actatattag atttacaaag atacccgcta
    46921 ttactacaga agctattgta actgtagtaa atccaccaac aagcatcttt ggaagcattt
    46981 tatccattaa aaattctttt tcttctggtg tttcagccag agcctttact gcttcttcag
    47041 ttaatatata gtttggcgga aaaccgtaaa gtgatgtaag tgaaactgaa aaagccattt
    47101 ctttgctttc acctaaaagc tttcctgcta taatagaaaa aattcccata cctattacac
    47161 caaatattat gattcctaca agaggaactg ccatttcaac aagcattgct ggtgtagctt
    47221 ttgctaaccc tgcaaatata aaagccatca atgatgtcat taaccatcca aatgaaccag
    47281 atttattaag tggttttcta tcaactattc caagttctga tgctattaca ccaaatatta
    47341 aacacataac atatcttgat acaacttcat ttgtaagatt tgtaaattgt actgctaacc
    47401 atgctacaaa ccctaatcta agtattatca tatatgtcgt atcatatttt tctggtaagt
    47461 ctggaattat cttaaatctg cttttttgtt caggtaaatc cttaactttt tcttcagctg
    47521 tatttacagt tacttttcca cttctaaaat ccgataaaag tctttttcct tcttttttaa
    47581 gcattaatgc tgtaatagga taacctgcaa atccttgtgc aacatacatt aaaattgcaa
    47641 gtactgacaa acttgcaaga ccttttgcag tagcagcatc tgccatcatt atagatgcaa
    47701 ctagccctcc agtaagagga ggtgtagcta ttactgctgt atcccatcca aatactactg
    47761 tccccaatgc caaagcacct acacatattc ctactattcc tgccaaggct attacgattg
    47821 ttttccattg acctgctaat tccttaatac tcatcatagt tcccatatga gttattaaca
    47881 aaagcatacc taattgagct agtggtgttc ccaaagcggc taagtccaca atattttctg
    47941 ggaaaaaagt ccaaaatcct actataaata taattgctga tacaaaaacc gacggaacaa
    48001 aagctttagt ttttgctgaa actacatctc ctatcgtcag agatattaaa actaaacaaa
    48061 aagctgttat tgcctccata caaaatcccc cctcataata aatgtatttt tataaaatgt
    48121 atgtatagtt atccattatt ttactacttt tgaaaaacat ttcaagtcaa atttcgtttt
    48181 ttttcataaa attctataaa ttctacaaat taaagttagt tttctaaatt attcttttta
    48241 taaaaacgtt atccctcgga ttggctattt tactctattt ttacaaggag aattaagtgt
    48301 aattttcaaa aaataaaaca tagaacatat tttattcata aaaccttcca ttaatataaa
    48361 atgaattgtt tttatgaatc ttaatatatc ctatgtttgt aagtttaatg cgtatgttag
    48421 ctatagtgta aaacttatat actagttatt gtgtaaccta ctatatactc catttttgtt
    48481 tactaactct tcatgagtac ctctttcttc aattccttta tctgtcaata ctatgatttc
    48541 gtcagcattt tttatagtag tcaatctatg tgcaacaact aaatttgttc tatctttact
    48601 taattcttct aaagactctt gaatttctct ttctgtgaca ttatctaaag cagatgtagc
    48661 ctcatccaat attattattg gaggattctt taaaaagatt cttgaaattg aaattctttg
    48721 tttttgtcca cccgataatt ttactcctct ttctcctata taagtatcat atccttctgg
    48781 taaagtctct acaaagtcat gtatcctagc ttttttagca gcagctatca tttcttcatc
    48841 agttgcatta gggttcccac aaagaatatt atctcttata gttcctgtaa ataaaaatac
    48901 atcctgttga actataccta tattttttct caatgatttt aaactgacat ctcttatact
    48961 ctttccatct atttttatat cacccttatc aaattcataa aatcttggca acaaattaca
    49021 caatgtagtc tttcctcctc cagaaggtcc aactaatgct atagtcttac cagcttttat
    49081 agataagctc aaattatcta aaacttgagt cttatcttca tatgtgaaag atatattttc
    49141 tatctcaata tcacctttta cattttcaag ttcaataggg tttttagctt ctttttgatg
    49201 gtcttgttcc attatctcta taaatctttt aaaacctgtc ataccatttt gatattgctc
    49261 tgtaaagttt attaattttt tcataggttg tataaacatt tttacatata gtatataagc
    49321 agcaaaatca cccaaagtaa tttttcctat ataagtaaag taaccacctg ctattacaac
    49381 tacccactct aagatatcca tgtacaaatt cataccagag tagtattctg ccattacttt
    49441 ataagaatct tgtcttgctc ttctaaataa tttattgctt cttacaaact tatttaactc
    49501 ttctttctca gtacaaaaag acttagttac tttcatacct gctatactat tttctaaagt
    49561 agcatttaca tcacctatag tgactctact cttcataaaa gcaatattca tcttatcttt
    49621 tttagctatt gcaaaccaaa ttataaatgg taatatagca aatattataa gcgtaagagg
    49681 aatatttata tctataagta taaagaatga accaaccaac attactatag agatgaataa
    49741 atcttcaggt ccatgatgtg caagttctgt tatatccatt aagtcattta ctattctaga
    49801 catagttact ccagatttgt tattatcaaa atacgtattt ggaagtcttt gtaagtgtgt
    49861 aaatacttca tttctcatat cagcttgcat atctacacca ataacatgtc cccaatactg
    49921 cataaaatag ttaagccctg cttttgctat aaatataagt ataagtgcta ctgcaaaaac
    49981 cactaacatt cttaagtttt tatttggaac aacgtcatcc attatatttc tagttatcat
    50041 aggataaact aaatcacata aggaaaataa aaaagctgct ataaggtcta gtgtaaaaat
    50101 tttcttatat ggcttataat atttgacaaa ttgttttatc ataagtatta aatccctcct
    50161 ttattagtac ttctaaaaaa tacccataaa ttagtataac ataacaataa ctttaaatct
    50221 tttataaact tataaatgaa aacaatattt ttcataaaat tgaatattta tcatatagtt
    50281 gtatgcttga aataacataa aaagagctat atctctataa ataaaaattt ataaagatac
    50341 aactctatat ttaatactga acttttttta taaatacaaa actacttcaa tgcattcaat
    50401 atttttttat ccaatgccgt tgtatttata cctgttatat atcctgttgc ctttattgta
    50461 ggtatatcat aagttgttgg tagtatagta gtagatacga ttaaatcagc accttcttgt
    50521 tttgttgaac attcagcaat tttacattgc tcaatttctg cttgtatatt attttcttta
    50581 accaaccttt ctactctgtc acaaactatt gttgatgttg cgattcctgc cccacatgct
    50641 actaatattt ttttcatata ttgtcctcct acactttctt caatatttta taatttaaaa
    50701 tacaactgat aaatttccta tatctttatc tatataaata attatatcat tttatatcaa
    50761 aagaacatct taaaattatt cttttagtat gttttttatt tcaacattgc ttatacttaa
    50821 aatatcattt tttatacaag actctatgta ctttgatata tttttatagt catttaaatc
    50881 aaactgtttt atattcaagt tatattttat atcactaaca atacctacca aatcagactt
    50941 attagaaaaa atatcctcat atttatcttt tctagttact tccaatcttt taaatctata
    51001 gtttttatat ccttctatga taattaagtc catatcttcg tataattgaa tcattttata
    51061 taatggactc ttatctttta gttcttcaat atatgctact cgtttatctg atattataca
    51121 gacttttctc gccccagatt ttctatgttt gtaagtatct tttccttcct catccatatc
    51181 aaaatcgtgg caagtatgtt taataactcc tacacgatac ccttttttat ttaattcttt
    51241 tattatacct tcaatcaatg tagttttacc tactcctgaa taggagacaa ctgacaatat
    51301 attatacata aaattgtgtc ctttcataaa actatctaat aacaatatcg actaattgat
    51361 tttcttttat tgtttcattc tttttaatct taatcataca attacttctt agagttgcag
    51421 ataatctagc ttttgcatct ttcttatcat ttaactctac atacaatttc ccttcacttg
    51481 tatatagttt tccatttaca aatctatctt tatttgaaag tttattaaat tctcctttaa
    51541 atatagcttt tatataatgt attcccttct tatatgttaa atactcaata acaggaacta
    51601 ccacattatc aaatacaatg attgctgcat gtggattacc agaaagacca aacaatagct
    51661 tttctcccaa tgtagctaca taacaagctc caccaggctt aatgtttatc ttccaaaata
    51721 attctgttgc ccctattttt ttgaatacat ctggaattaa atctttatct ccaacagaga
    51781 caccacctgt tgtaattaaa aaatctgcat tctgtaacat atcccttacc tttttttcta
    51841 actccaatat atcatcagaa caattgcata ttgtatctgc ttgtattcca agttttaata
    51901 atcttgaata taaggtatat ctattactat cgtatatctt accattctct aaagtctcat
    51961 ttatatcttg tagttctgtg cctgtattca aaattccaac ttttaaatca tcataaacag
    52021 ttactttgga tattccaaga cttgctaata ctccaatacc atcatatcct atattctggt
    52081 ttttattaat aatcaattgc ccctttttta tatcctcacc cttaaatata taattatcgt
    52141 attcctttaa ttttacattt aaagttatca tatcacctat aaccgttaca tcttcttttt
    52201 ttattacaca gtttgcaccc cttggtattt ttccaccagt cattatcttc actgcttcac
    52261 ctttatttaa taccctgtta aatacttctc ctgcatacaa agtccctata acctttaaat
    52321 ttctattttc agaatttgca ttatatgcat aaccatcata tggtgaccta tcaaatggag
    52381 gactatcaat tgttgaatat atatcttctg caaccacttt atttactgca tctatgagat
    52441 ttactctctt gaccctgttt attggtaaaa catttttttc aataattttt atggcatctt
    52501 ctaattctat cattttaaat actccccaat atatttttta tttaagatat ttctatgtta
    52561 atatattata tattacaaaa tttggaaata cttttatctt atctttaatt ataactaata
    52621 gttatctata agtgcagttc aaattttata aatctaataa aaaaactgtt cccagctaga
    52681 aatttccttc tatcttgaaa cagaatcgta tgctttagaa tattttattc taataaagtt
    52741 ccttttatta ttttcacaat tttatcactt tcaatagttt ttatagattt tattttgtta
    52801 tccacaatac ttattgtaag tacctctttt tttaatattt tgttatcttc tttcaatcta
    52861 tagctcttaa ctactttact accttcatga ttatatgctg ttaaatcata attcgcatct
    52921 cctacaattt cagttattct agactcttca acaccaacat actctcccag gtcaatacgt
    52981 ctaaaatttt ctagtgatac agccgatttt tcatggtcac ttttttcaat cacaatacta
    53041 gattcagcat caacaactgc aataccttgt gatgagaaag aattagtttc aacattgaca
    53101 actttatttc cgttaatatc aacatataaa gcacttccat ctagttcgtc atttccagta
    53161 tattttggat aaactaatgt catataactt gatgtattta tatcttgcat tgtatttgat
    53221 tcttttaaaa ctttaggttt tatataataa aatgtattat aaggtatacc catattcttt
    53281 ataatatact tataatcttt attcataatt tcattaacat cattttgaac ttttgtcata
    53341 gttttttgct gtgttgcttt acttgctact tgatttttct ctttagtatt ttctttatga
    53401 catccaacaa gtataatacc taaaatagtt acacaaacta atgacctttt taacttcata
    53461 aatcaatcct cctttcttaa attttatagt atttatctat atatcaattt acagttatct
    53521 cttttttcaa taattatatt tattatatta taaaataatt atataatatt tatttgaatt
    53581 taataagatt aagaagctaa ttgtaatttt tctgtaataa aacatacttt aaagttgaat
    53641 tttgaaaatt caatttacga tatttttatt attaagtacc ttacaacatt ataccaaatt
    53701 attcctaaac aatcaaaaac tccatgaata tttaaaggtt ttctatttta aaaatcacct
    53761 ttgactatta atttatcaaa tatgttataa tagttttaga taatttaagg aggattaaat
    53821 aatggatttt aaacaacttg aagtatttgt tgctgttgca aaacatcaga gtttttcaaa
    53881 ggctgcaaga gaactttttt taactcaacc tactgtaagt gcacacatac agaatttaga
    53941 aagagagttg gaaactgttc ttataaatag aagcaacaaa gttattacac tcacaaaatc
    54001 tggtgaaatt ctttatgaac atgctatata tatattaaat aattgtaaac gtgcaattta
    54061 tgatataaaa gaatattcag gaaaaataga aggtattata gatatagcat gtagttccat
    54121 ccctgaaact tatattttac ctgattttat gaagagtttt tctatgagtt atccagatgt
    54181 aaaattttcc ataagccatt atgattctca atatgctata tcagaaattt taaatgaacg
    54241 aataagcttt ggattagttg gttctaaaat caataatcct caaatagagt acctagactt
    54301 attagatgat gaacttgttc ttatcactcc ttcagatttt aaaattgata ataaaaataa
    54361 ttgtatcgat ataggagaat tggcttatct gaattttatt atgagaaaag aagggtctgg
    54421 tacaagaaac ttaatactaa acacacttag taaaaataac tttccagtaa ataaattaaa
    54481 tgtaatagct catgtagaat caaatgaggc tattaaggaa atggttaggt taggtcttgg
    54541 agtatccttt atatcgtaca tttcagctat tgattatctc aatgcaggca aaataaaatg
    54601 ctataaaata aaggatgttg attttactag aaaatttttc tttatttact ctaaaaagaa
    54661 aactttttcc ccattagaag ataaatttct aaacagatta tgtgagtatt tcgaaatcat
    54721 aatataataa ctttaataaa acattgctta attttagatt tacaaccaat taataatcta
    54781 attataacta tcacataaat acttataaat tattataaat catctttctt ttttatttaa
    54841 tttagtcaaa aaatccctca ttcaaatgaa cttgggattt ttttgtttat actactccaa
    54901 tagcagctaa tactatacaa ataatcataa ctgaaattag tatcgtagtt gtctttacct
    54961 tttttcctaa taaccaatac ataataccga atatcatagc tggaagcata catggcatta
    55021 tttggtctaa gtaagtttga ataggctctg cccattctcc actacctaca ttaacactaa
    55081 gttttaacat tacattagat gcagtcatac ctccaattac cataagtcct aacattgaag
    55141 ctgcttccat aacttttgaa actatacttg aaccactcac atctttaaag aattgagttc
    55201 catacttaaa tcctttatct agacatatat aacgtataat aaaatgagga acatttatta
    55261 ttaaaaggaa caaaatcggt cccattatgt tgccctgaga agctaaagct ataccaactc
    55321 cagctgctat aagctttaat gttccccaga aaaatgaatc tcctattcca gaaactggac
    55381 ccattaaact tgatttaaca gcatttatac tattagcatc aaattctttg ttctttgcat
    55441 tttcttcttc catagctcca gatataccta ccaaaagagt acttatatga ggagtaactg
    55501 acataaattc taaatgtctt tttaaagcct tagatttctt ttctttatca tctttataca
    55561 atctacgtat tattggtgcc atcatgtatg catatgctat attttgcata cgttcgtaat
    55621 tccaagatga atctaatgtc aatgaccttc taaaacattt ccaaaagtct ttcctcgtta
    55681 ttttactgtc tggtgaaatt gtctctaact tcttagaact cgttgtcatc tactattacc
    55741 tccttctctc ctacaaagtt aacatatatt aaggctattg ctaatccaaa caatgcaact
    55801 cctgttactg ttatgtttaa gtaagcaacc aatataaatc ctaataataa aaatgctact
    55861 gttttcttat taagcatcat tctagctaac atggcaaaac ctattgctgg tatcacacct
    55921 gctgcaacat ccattccatg agttacatat gatggtatga atgaaagtat tttctctatt
    55981 actggaacac ctaatgaaaa tcctattcct actattattg ctaatatcaa tttatttcct
    56041 ataccaaata ataattgtat acgctctaca ccttcaatat tcccctcttc tgcataacgg
    56101 tcagcaaggt gagctcccca agtaagtact actgtcatca agaagttatc tataagcaat
    56161 actagagttg ctataggaac tgcaagtaca actgctgctg atacatcttg accagttaat
    56221 attacaaatg ctgtggcaat aatagaccca gatacaaaat caggaggatt acttgctcca
    56281 acaggaaatg cacccataaa tactaattct aatgttgaac ctattactat acctgtgtgt
    56341 aaattaccta gtgctaaacc aacaagtggt gcaacaacta taggtctatt atgcattgta
    56401 gaacctacaa aactatgcat atatgcaaag aatgcaatta gagttatcaa aataacttgt
    56461 gtaaattcca ttttcatccc cctaaaatta tattaatttc attacatttg tagcgtcatc
    56521 atcaggaact aactgtacaa atacatttac gccttctgaa tctaactctt taatcatatt
    56581 tatatcatct tttgatacat gtactgcttt agatatattt tcacgattat catctgattt
    56641 tgttccacca aggtttatag atttaatagc ttttacctct ttagcaagtc tataaacatc
    56701 ttctacagat tcacaaagta tcaataagtt atatttatca gtaacacctg agttaagtgc
    56761 ttttatagaa tctgctatgc ttttcattac tagcttaaca ttagatggtt tagccatttt
    56821 aagtcctgcc attcgtaact catcttttaa taattcatca cttgctatta atatacagtc
    56881 tgtgcttaaa aactttgtcc acgtaaatgc tacttgtcca tgtataagtc tgtggtctac
    56941 tcttactaat ttaatcatta taattcctcc ctaatttttt tgatttcatc catgaatgtt
    57001 tttacgtgtt ctacactaac atccccaaaa tctttgtaat tatctttaaa gtaacttcct
    57061 attattgcca tatcacctaa ttcaagctgt ttttttgcat tttctaatgt aactccagca
    57121 gctataacca atgggaaatc tcctaaattt ttacgaaaca gttctatttt ttccattgat
    57181 gtttcttgac ctgtagcatt ttctgtaact gctatagcat cacaccttgt cattcctatt
    57241 tttaaatctt cttcaatatt attttctgat aatactggtt gatatttaaa acgtacgcct
    57301 ccgattaaat atgctggaca tttcgaacgt tgtaatttaa aaaattcttc taaagtagcc
    57361 tcatctctag gtttaacatg acctactaca gaatcaacct gtatataact ggcattatat
    57421 tttgttgcca gttcgaatcc catagtatca acatttaaac agtttactcc atatggtatt
    57481 gataaattag cttttgatac atactcaagt attctttcta aatcataata attaccatag
    57541 taattttcta acattattcc atctacacca ttttcttcaa aaattgcaat ctctttttta
    57601 gctctttcaa agatatcttc tggagtgtct ccttttaagt gtatcattgc tataattggc
    57661 tttttacttt taaatactga taatattccc ttttttttca taaactaaaa tacctcttct
    57721 tcttctttaa tttgtaatgt cacgctttgt attcctcttc tagattcttc tactaattgg
    57781 tctatatctt caactgttaa ttcatcagaa aatcttgtaa gaagttctag taccatattc
    57841 aaattcattc cagaaaataa cttaacatta tgtagattag ttaatcttaa cattgctgta
    57901 catacacttg ctccatataa gtcagataaa attatatatt cacattcttt atcttctgat
    57961 attgtttttt ctaattctaa agcaaaatct gaagcacttt gtccagggta taaaccatac
    58021 gaactaacac aatctgctaa atctcctact atcattttta ctgcatttaa aagccctttt
    58081 gaaaagtctc catgggatgc taatattatc tttttcatac ttatcctcct actaaatata
    58141 aattatattt ctgctgatac tgcatatttg tacttatcac ttcttataaa ttgctcattg
    58201 taatataata catcattatt ttctgtatac accgattgtc ttactttcaa tagtggcata
    58261 ttacttgcac atttaagatg gtcagaatat tctttgtttg aatgttttac ctcaaatata
    58321 ttttctactc gcttaggtaa tgtgcttata ttattagata taacctccat taaagattta
    58381 tcttctagtt cttcttttaa taaaaacata taatctattc taaaataatc aacttcaaaa
    58441 ataacaggta cttcatctat taatctaagt cttttgatac atattacctt ttcttctagt
    58501 tcaatactta aagcttcagc tacttgctta tcagcttttt ttattaaaac agagattatt
    58561 ttagtgcttg gttttgcatt aatacgatga caagaattag taaaactatt tcctgcacat
    58621 gttgcttcta ttctctctgg tattgttaca taagttcctt taccacgttt tttacataat
    58681 atatcttctt ttacaagttc attgagtgca tttctaactg ttatacgact tatattaaat
    58741 tctttacata agtctccttc agatggaagt ttatctcctg gtttatatat gccagatgct
    58801 atttgttttt ttatgttgtc atatagttgt tgatataaag gaacaaagga atttacttct
    58861 aaatccattt cttttttttg tctcttttca tccattttgc tcacctccct atgtaacatt
    58921 tttataacat tatataacaa gttctttatt acatattata acaagatgaa atcgttgtca
    58981 attttttttt aaataatttt taatttgaca gtattttatt ttattttctc cagtgtgaca
    59041 tattgctatt tttacaaaaa aataagactg ttgtacaaca ggttaaattg tttatacaac
    59101 agtctttatt aacttattat gcttatattt ttacttataa atttatatta aaaaatgcct
    59161 tatatccctt atacatttca tatatgtcta ctttagagac aatctcatgt ggtgcatgca
    59221 tatttaaaac tccaacacca caatctataa cctctgcacc ttggtttgcc aatatatggg
    59281 ctatagttcc gcctcctcct tggtctactt ttccaagttc agcagtttgc catactacat
    59341 ttcctttatt aaaaattctc tttacttctg acataaattc agcattagca tcattacatc
    59401 cactctttcc tcttgaacct gtatacttag ttaaaactac tccatttccc atatatgcag
    59461 aatttctctt ttcatacgct tctccaaaat ttgggtcata tccagcagaa acatcagctg
    59521 ataatacctt actatttgct aaagcccttc ttactttaat atcacaataa tctccttcta
    59581 aagcaatcag ttcagccaca gtattttcaa aaaattttga gtgcatacct gtatttcctt
    59641 tagaaccaat ttcttcttta tctacacata atgcaactga agtaaactct ggttgttcag
    59701 tttctaatat agcttttaca gcagcatatg aacacaccct atcatcatgc ccatatccca
    59761 acaccattga cctatcaagc cctaaatctc tagccttacc agctggaact acttcaattt
    59821 cagcacttaa taaatcctct tcaactatat tatatttttc atttaaaatc tttaatatat
    59881 ttgctgttat aggttctttt tcttcatcat ctaaaggaat attcccaatt aatatattta
    59941 aagcttctcc actaattcct tctgataatt tcttttgcat ttggtctcca gccaaatgaa
    60001 tcaataggtc agttacacaa aatactgggt cattatcttc ttccccaata gatatctcca
    60061 cttttgttcc atcatttaat ataacaattc catgtaaagc aagtggaaca gccgtccact
    60121 gatatttttt tattcctcca taatagtggg tcttaaaaaa acctagatta ctctcttgat
    60181 acaatggatt tggttttaaa tctaatcttg gtgaatctat atgacttcct ataatcttca
    60241 tacctttttc tatataattt ttacctatta caaaaagtgc tactgccttc tctttattta
    60301 taacataaat cttatcacca caacttatgc ttcctttttc catagcttca tctattgaaa
    60361 tatagccatt ttctttagcc aattttataa tttcttttac tgaaagtctt tcagtctttg
    60421 acttacttaa aaattccata taattgcgag aatattgcat aatttgttca atagtatttt
    60481 cttttctttc aaaatcccaa gcattcttaa attcgtactt taagttcata ctttcctcct
    60541 actaaattct aatttttatt gtttttcatg tattgctcat attcttctgg tgaaactact
    60601 ttgacactat ctagtcttga cctaaagtgg gctctgaaat tttcaagata ttctttcctt
    60661 aaaatttctc tttctttgat ttcatcagca gaaaggattc cctctttatt tttctttgct
    60721 aattcattaa tcctatctaa tttctttttg tcaaacattt tttcctccta aaaacttcta
    60781 tttatttaat aaaataattt attgcttttt atatctaact tgtttttata ttcacaaaaa
    60841 aactcatcta tattatcact tattatagat attccctttt ctagctcttc catgcttgga
    60901 cgagctatat ttattctaaa aaatctatca tctactagat tgtcaaaaaa ataagtccct
    60961 ggaagcattg acacaccttt gtttaacata aaatttgtaa atgcttgaga tgtatatcct
    61021 cttggtaatt ctaaaaaaaa gtttattcct ccacaagatt ttctaacttt taatttacca
    61081 ctcaatttct cattaatcaa gttttttgcc aatttatact tatcagtata tatcttctct
    61141 atatgactca aataattttt ccaatcaaaa ttattcatat agtaatacat tgatttttga
    61201 attaaacctg gtgttgatat atctgatgaa tatttagccc ataaaacttt ttttaacaac
    61261 tcatttggca tttctacaat tcctatccta agacctggca taagtatttt tgaaaagctt
    61321 tttatatata ctactctatt tttatcatca taacttctaa ttgttctatt gtccattgat
    61381 tcaaaaacaa aatcacttat aaaatcatct tctattatat aaaaatcatg ttcttctgcc
    61441 aattctatta atttcttttt tttatatgtt gagtaagata ttccggttgg attttgaaaa
    61501 ttaggcataa tgtatataag tcttggtttt attttttcca actttaattt taaaatacct
    61561 atatcaattc catcatctag cataggtata gatattattt ttgcacctct gcttttaaac
    61621 acttcaattg ctccattata agaaggttct tccataaaaa caacatctga ataacttata
    61681 agacctttac aaactatgtc tataccttgt tgagcacctg atattatttg aatattttcc
    61741 ttagtagttt ttatatttcc atcacttaga tactccacca tcttttctct taattcttca
    61801 gaaccaagac cttcatcata ttcaaatatt gagctaccgt ctttagataa tgccatattt
    61861 acagcatttt taaatgaatc aataggaaac atatcattag atggattccc attgtcaaaa
    61921 tgaattaaat cttctctttg cacaataatc ttattttttc tgttatctat accgctttta
    61981 taagaattta aactagatac aaaagttcca ctccctataa ttttatatac atatccttcg
    62041 ttttctaaaa gttcgtatac cttaactata gtggtgttgt ttacttgtaa aaaatttgat
    62101 agttttctta ttggaggcaa tttttcgtgt tcaactaact ctttatttat tatcaattct
    62161 tttatatgat taaatatttg tatatattta gttgattttt tctcatttaa atctattctg
    62221 tatttttcca aatttatcac cgctcattgt atttatattt tattgatgct tttacgtata
    62281 tttgatatta tttagtatgt atatataata ccaaaatttt tataaaaagg ggaaatattt
    62341 atgttttacg actatcacat gcactcaagt ttctctacag acggaaaatc tacaatggag
    62401 gaaatggtaa aaaaatctat tgaacttggt ttggaggaaa tttgtttcac agaccatgtt
    62461 gactatgatg tatatgctga tgattccttt tcaattgtct acgaagatta ttttaagagt
    62521 ttagagactc ttcaaaacaa atataaagat aaaatatcaa taaaaaaagg aatcgaattt
    62581 ggagttcaaa ctcaattaat agatacatac aaaaaggaag cacatcaata tccattggac
    62641 tttataatat gttctatcca tgctatagat acaatggact tataccttgg taattatttt
    62701 aaagataaga ctcaacacga agtttacgaa aattactacc tttatttata taatattgtt
    62761 aagaattata aagattactc tgtcttaggt catttagatt tgattaaaag gtatgcacct
    62821 tatgacacta ttttagatga tagattattt tctgatataa ttgaagaaac ccttagacaa
    62881 gctatttatg atggcaaagg tatcgagata aatacctctt gttatagata taatcttcca
    62941 gacctaaccc catctaagta tatccttcaa atgtacaaag acttgggagg ggaaatcata
    63001 actacaggtt cagattcaca tcatataagt caagttgcat gtgagtttga ctacatatat
    63061 tcattactaa aaaatatggg atttaaatat gtatctaaat ttaataaatt gaaaccagaa
    63121 tttataaaat tataaatttt tacacctcct tgtgaatata tttaaaagat aaggaggtga
    63181 ttttttgaaa gtaccttatt tagtactatc aacagttatt ttatttatat ccaattttat
    63241 tgtaagaata tttggttttt tatataaaat attcttatca agagcactag gtgaaacagg
    63301 tctaggtatc tatcatatga tttttaactt tttgatgatt tgcttagctg ttacaacaac
    63361 aggtatacct actgcactta gttgtctagt tgcaaaaaga aaagctttaa atgatagaca
    63421 caatactaat gctttattta tatcaacttt atatatatca tttttcgtag cactaactat
    63481 atctatagtt gcatctttca acagttcttt tttatcaatt aagtttttaa aagatgctaa
    63541 attaaaccta tttatactag ctgtttgtcc tgctatagtt ataataacta tttcaaatgt
    63601 actaagagga tattactatg gcataaagaa tgtaaaaatc cctgctattg gacaaatcat
    63661 tgaacaaatt ggtaaaatat tatttgtttt tttacttgtt atgtacataa ataataaatc
    63721 tatgaattgt tatatagccc tattaggtat atcaattggt gagttgagca acattatatt
    63781 tatgcttatt tgcttatggc gagattcatc atttgacaat aggtatatta taagcataaa
    63841 agatttttat aattcttcta tggaaacatt aaaaatgtcc attcccataa cttgtaatag
    63901 aatgtcaaac atacttttgc aatctataag ttctatgatg ataccatcaa gattagcatt
    63961 atcaggaatg acttatcaac aatctttaag tatgtatggc gtcatcagtg gtatggtaat
    64021 gccttttata tttttacctt ttactgttgg ttcagcgctt attgtaaatt taataccaac
    64081 tatttcacag gaaatggctc taagaaagcg taagagtgta attaaaaaaa tcaaatactc
    64141 aatacttctt actttatttg taggcatact atcatcaata tttttttatt tctttggaaa
    64201 agatttatgt atattagtat ttaaaaatac acttgcagga gaatatctaa aagctatgtt
    64261 tttagttcca ctatttatgt cactaaatca aactctatct ggcattttac attcaataag
    64321 aaaagaatta gcttcaagta taaatactat cactggtatg ttaattcaac tgatagcttt
    64381 atatgtattt cttccaattc caggacttaa tatttatgca tatatataca caatgacgat
    64441 agtatcaatc tttacttgtc tattacatac tatagtgctt tttaaatcac taaaagctat
    64501 acactagtta taaaaacgtg aaggctacca atgtaaatta tacattttgg tagccttcct
    64561 tagatgattt ttatttataa tttaaactgt ctatatctct acctatctat ctgtggcaac
    64621 ttggctttcc actttcatga ttaggtccac attgcgaaaa atcacatact ttagagaaag
    64681 tatcaacaag tcttggttgg attggatttg gtgatgctaa aactactatt tgttcactta
    64741 ttaatgtgct aactgtattt tgtaagcaaa gtactaaatc tatatcattt ggtattatta
    64801 attcatagaa aggttcacaa cattcgttac attctaattt taatatagat tttcctatac
    64861 aatctacagt taagttagtt tgatattctt gtcttaaagt aacagctctt ccgtcagctg
    64921 gcaagcatat tggtgctgaa agtgtgttaa aagtaaattc tacataatca actcttctct
    64981 tacatccacc tcctgctcta actgctggga aagctaatgc aacgaactct gtacatccac
    65041 atctacctct tagctctagt actaaattac gaactgctac agttaatcct ctttgtttat
    65101 aagctactga ttttcctttt ccttgtctac agcactcagc atttctttct ccacaaactg
    65161 caaattcaaa agttgtttca cagtttcttc ctactatatc tcctaatggg tctaaatcga
    65221 aatcttctaa agttgtcatt cctgtatcta taactattcc atcattactt aaacatattt
    65281 tttctatagt tacacttgct ttacctgcac ttggaactga accaaatact tctactactt
    65341 ctgtttcaaa agttaatggc tctccatttg gtcctgttgc atctgtaaat gtttggaatt
    65401 gcatagtatc aaatattcta ttagcctcta ttgcaaatgg cactgcattt gatacagaga
    65461 agtttcttgg agatatagga ttacagcatt ctggtccaca tttgttttca aaacattcat
    65521 ctgaatccat ttcacatggt tcacaatcac atttacaatg gtcatggcat ccacaattgt
    65581 catcacatgg attaggtttg catgggttgc atggattagg tttacatggg ttacatggat
    65641 taggtttaca tggattacat ggtttgcaat catcatgaca atcacattta ttatcagtat
    65701 tacattcatt atgatgatgt gattttttaa accctctcat atcttctgaa tttatttcct
    65761 ctgtatacac ttcttcttca ttcattgtag gcattggctg atttgtcatt cttcttttat
    65821 tttttttata atcttgcatt ttttattgcc ctccctgtaa gcgattctta ttaagtcttt
    65881 ggctttttaa agcctaatta atactatgct cttttgagct taattgttac ttcgcttttt
    65941 aaatctatca ataattagtc agatttagta aaaaaatgca tttttaataa aaaaagtact
    66001 actatttgaa atagtagcac tttttttgtc ccgaaacacc ggtggctaag taaattcaca
    66061 ccctaggcac tcccaggtgg gtgacttgcc agtaaccttt aacgtccaaa ctaactaggc
    66121 atgttataat ttaccagaca cggtctcccg tatttcattt gtaggttgaa tttaaaaaag
    66181 ccttatcgta tgtttcagga tttcatatta ttattatata ataaattttg aattttttaa
    66241 agtggtttta tatagtaatt gagataatct tattctatca ttactttttc ttcctttttt
    66301 ctttatgtat ttagttaatt atcgtttttt cttagttctg gttttagtat tttttgcatt
    66361 ataattgtat tcggattctt gctcattaat gttgtatagc tcctcatcat atactatatc
    66421 ttcatcattt tctatatttt catagtctgt tttattatcc tcgactaatt catcttcatt
    66481 atcctccatt tcatcttcgt ctgttaactc ttcatcatca taatccaatt ctttgtcttc
    66541 atagtctaat tcctcatctt cattattcaa cccttcatta ttgtcttcat cttcattatt
    66601 actatcatca cctcttaaaa gacttcctaa caatttgaga ttattcatac tgccaagatt
    66661 gtttatattt gccaaagagc ttaaactaaa tccgccttca ccaaagtcat ctggtaaaaa
    66721 taattctgca aataaatcta tcttcttttt cattcctata gctttttcaa ctgaattttt
    66781 tcttttttct gggaatacat ctataatttc ctttatcatc atattctttt tatcatcact
    66841 atccattttt ctgaaaaaat ccgtttcatc ttctatatct accatatcta tattatttaa
    66901 tttttttatt cctctaacca aatctaaaac actttctatt tttattagta tttttctttc
    66961 atcttttgac atgtactttt tagttcttgt tattatttcc attcccattt ctaaatcttc
    67021 ttcacttaaa tccatgagcc ttaagcctct ttttatgtca tttgcatcaa accttaaatt
    67081 gctatgtgta gactgtgaca gtttatcatt tgcatttttt ttgttttttc taactctatt
    67141 atttcctttt gatttatttt tatcatcaaa tatagagata ttaccattac tcaatgcaat
    67201 cactccaatc aaaaatagta agttattcaa gacaattccc ccttatatta tataattgcc
    67261 ctatattaaa gtatgttttt catattttaa tgttccattt ttaaagctca gtgcatatat
    67321 attatctata agaattttta tttatattat atatcactca caattcacca cttcaatatt
    67381 atttctatat ataaatagta tgaatataat tcaatatata tttatagaaa ggggtttttt
    67441 tatgaaaaaa ttagatgcag agtcattaga aaaaatggct aaacaaaaaa atattgacaa
    67501 ggataaaata gaaaagatgg ctgatagtta caaaggaaaa tcagaaaatg aacttatgga
    67561 ggaacttata aaaataggta aaaacttaga tggaagagat gaagtagtat caaaattcaa
    67621 agcgttttta gatgaaaatc aacgcaaaaa attggatact ataatggaaa aaatctcaga
    67681 tgcagaaact cagagagata ctaaaccagc taaaactaag aaaactaaac ctactaaagg
    67741 taagtcaaat tcacctgcac caaaaaatac accaactggt caacagaaaa aatctaaagg
    67801 tttatttaag aaaactaaaa agtctaaccc tcatgaatag ataaaataaa ctatttaaat
    67861 aattagacaa ttatttttag ttaatctagt attaaatttg atattattaa caagaaaaat
    67921 caggtagtgc tctaagcact acctgattga ttttaatagt tttatttttt tctttctatg
    67981 acactttctc tatcaacaat tctataaggt aattctattt tcttattttc aattaattcc
    68041 ttatttatta actttatgac cattcttatt gctactgctc ccatatcgta taaaggttga
    68101 tatactgtag taagtttagg tctatacatt ttagctatct taacatcatt aaatcctgaa
    68161 actgatatat cttctggaac tttatatcca gcatcacata tagcatttat agcacctata
    68221 gcagcctcat cacctgttac aaaagccgca tgaggtatta ttccatcatc taatagttct
    68281 ttcatactat tgtatccact ctcataatca gttccaccat acttaatcaa actcttgtct
    68341 aattctatat tgttgtttga aagtgctttc tcataaccag caagtctttc catttctaaa
    68401 acagtatcat ctttactagt cattataaaa gctatctttt catgaccttt ctttataaga
    68461 tgctctgtca tatcaaaagt agcttcttcg ttactagtac ttactgtata tatatcataa
    68521 tttctagcag ttttacttat atatgtcgct ggtattccac aattttgtat atagttgata
    68581 tgctcttgtt caactatcca cgaaatcata actatacctt ccacttgttt tgctctcaat
    68641 agatttatac tcttaagttc ttgctcttta tcagagtatg tattcgctaa aagaatatca
    68701 tagtcataca ttttagcaac ctcttctatt ccatttaaca cctcattaac aaaagaatct
    68761 gaaacttctg gaactattac ccctattaat tgacttttct ttgttactaa acttctagca
    68821 agcggatttg gtatatatcc agtctctttt ataacctcta aaactttttg tttgacttca
    68881 tcagttacag gttttgaatc atttataact ctagatacag tagatattga cactcctgct
    68941 tgtttagcaa catcttttat cgttatattg cctttcatct tcatcctcct tattttacta
    69001 aatttatcta aaactgaacc aaatttttga aggttcctat tttaactaca tttatataag
    69061 tttttatttt accataaaag caagtacatt taaatacatt atattttata ttatacccta
    69121 tattcaacaa atttaataat attcattctt aatttaaatc atattaaaaa ataaaataaa
    69181 aaaaatactg gtataaaata ccagcatttt ttattataat tatttcagta ttagcagccg
    69241 cagcccactt caccatcaaa gaatccacca cttgaacaag ttattagtaa taatattaag
    69301 aaaggtatcc atgacattat gtcaatgtct ctccagcaat cttcaaaagc taagaatagg
    69361 aagaatagta ctattatcca ccatcctcct ccaccaaaac atccttcttc tccaaaaaat
    69421 ctatctaaca taacaaagcc ctcctttttt taataactgt actcttattc taagcatcca
    69481 ccacagcagc aaagtattaa taaaactatt aggaatggta tccatgccat tatgtcaaat
    69541 tctgcccagc tatctccaaa agctaagaat aggaagaata gtactattat ccaccatcct
    69601 cctccaccaa aacatccttc ttctccaaag aatctatcta acattatacg ttacctcctg
    69661 ttgatatttt gttgagcttt tcgctcctaa catatactat gcattgggtt tccattttgc
    69721 taatgtattt ttattttttt ttaaaatttt ttaatctgaa ttaaaaaagg caaaaccata
    69781 acttgtataa gttactggtt ttacctattt aataatcaat attttttagc aacaacaacc
    69841 atcatcgaag ttaaataata acaacactat taatataacc catatccata tattgtcatc
    69901 acataataaa tcttctaaaa tgtctgtgca taataataag aatactataa ctattattat
    69961 caacatttta ttaccaaaaa atccttcgca tcctccaaag aaacgttcca tattgtcttc
    70021 taattttgtc ataactaatc ctccttgaat cattaagaga ctaagttttg tctctttaat
    70081 taaatatatg tagtaataga cagataagtt acttctaaaa aatttttatt gattagttta
    70141 tttatttctt tttcaccaag tttattgtat ctatcaactt catcaagata atcttttttc
    70201 tcctctgata tagtatagat taaactttct attgatttta ttttgtactc cttatatctt
    70261 tctatcaaga aggatgtttt tgaattttct attcctttct ttacatgcat taaccttgat
    70321 atgtagtctt ctttttcatt agatacataa ctctcctttt cagctacatc ttcaaggatt
    70381 aaattggtct ccattacata atcctctata cgttgcatat ctaaagttat tgaattataa
    70441 taaaaattta ctgctataag taaaaacatt cctattgaag ttagtaatag tcctacatat
    70501 ttcatagcat ttcctcctaa gttatatttg ttatcaataa agtttattca tcaaataata
    70561 ctttattact acatttataa aattttatta ttttttatag attccatatt gaaaaattct
    70621 tgatattata ttaaactaac attaaaatta attaaaactg ctttaggagg cttaaaatgt
    70681 ttgaaaaaat aaaagaaata atagcagaac aattaggggt agacaattta gatgagataa
    70741 ctatggaagc atctctaatg gatgacttag aagccgattc attagatgct gttgaaatta
    70801 ttatggcact tgaagatgaa tttggaatag agataccaga cgaggaagct gagaatttta
    70861 aatgtattgg tgatatatgt aaatatatcg aagaaaacaa ataatatcgt tttgtctaca
    70921 aatataaata gtctgtgtaa acttctacat aataaattat attagagttc acacagacta
    70981 gattagattg attcacaata tacaagagat aatctcttat atttaattta tacaaatata
    71041 taaattactt ttcttcataa aacgtcacat tctttattat tatatctata gtcccatctt
    71101 gattttgttt tacagtatac ttcattgggt cttcaaaatc agttagattc ccttttatat
    71161 caaaaccatt atccgttttt atacttcttt ttttaagctt tttctcaacc cattttttat
    71221 ctatactaaa actttcatca agaccttttt cttccatatg ctctttaaag ctatctttta
    71281 actcatcatc tttaattgaa ttatcaacaa agtcatttat atcaacttca tgtttttcct
    71341 ttagtgtata atttaatata cttcttacat cttcagcttg ctttatatca ttactaagag
    71401 cattggttat ccaattttca gctgtatttt taaacacctt ggtcttgtac ttatcatcat
    71461 ctattttttt agcatttaaa aattctgtca caaacttaga ttctgaccct tctttttcag
    71521 catccttatc taaaagcctt agatgaaact catcatttat gccacttaaa ctaatcaaag
    71581 ctccttgctt ttgtcttaaa gtctctggta taccaatttc atttggaacc atttgaatat
    71641 taaatttatc atctacaaat tcaattgaat gagtgtacaa ctttttataa tctaatttaa
    71701 gtatagctac actcttttca tctttaatag tgtataaaca aattgccaaa tcacaagatt
    71761 ctagtgcagc attaattttc ataacatcaa acaaataaga tgcaatttct ttagaatttt
    71821 tcaaaaaagt actttcatca tatattattt gttcacaaca gtttttaatt aaattatcat
    71881 tataatcttt aaaaacacct tttcttaaat cctcatcttt tgcaattctt tttatggctt
    71941 tctggaaaaa accatctacc tcttgattaa ctttgccttc aaagtcattt aatattggaa
    72001 catcactatt tttgtccaaa acatgtatta taaatttatg tattatcata tatatcaccc
    72061 cattatctta atttcgatat taactctttt atatcctctg gtatatctgc tttaaatttt
    72121 aatttttctt ttgttcttgg ttgttcaaac tctagcctgt atgcatgaag tgcctgccta
    72181 tttataagtt cttcatctac acaaccatac aactcatcac ctattattcc atgacctata
    72241 tgagccatat gtacccttat ttgatgtgtt ctaccagttt ctaagcttac ttctaatacc
    72301 gttgcatttt ctagtctttc tattacttta tagtgagtaa cagatgattg tcctctttca
    72361 tctattatcc tctttattga atcttctgta ggtctataaa ttggctcatc tatagttccc
    72421 tcattttctt ttataattcc atcaactact gttatatact ttttttcaac tttattttca
    72481 ctcattgctg tggataaagt atgatgtgca taagcatttt ttgctacaat aactaaccct
    72541 gaagtattca tatctaacct atttacaaat cttattttta cattttcttt ttgattatct
    72601 atatagtaac taataccatt tgctattgtt ttatcatagt ggctttttgt tggatgaact
    72661 accataaatg gaagtttatt caccataatt atatcaaaat catcataaat tatctgtaaa
    72721 tttaaatctt gaggttcaaa atttgctttt tcctcattta tttttacttc tattaaatca
    72781 ccactataca cttttaaact cggcttttta tatactccat ttactaaaac actcttttcc
    72841 ctcttcattt ttgataatga cctaactgag aaatttagct tgtccaataa aacttctttt
    72901 aaagtcattt cttcttcatt tgtatacgat ataagattat atctttgatt ctctttttta
    72961 aacaaacttt tacctacttt cttttatgtc ttcctacttt ttttatatct ttaaaatcat
    73021 gatattatat attttacacc atttttgttt aatagataat aattattttt aactataaaa
    73081 acctgtaaga ataacaaata gttattctta caggttaaat tcatagttaa atatttaatt
    73141 tgtaaatatt ataatctaaa actatctctt aactattaaa ctagttccca ttccaccacc
    73201 tatacaaagt gtagcaagac cagttttagc atctcttctc ttcatttcat ataaaagtgt
    73261 agtaagtatt cttgctcctg agcatcctat tggatgtcct atagctattg ctccaccatt
    73321 aacattaact ttattcatat ctatatttaa gtctcttatt acagctacag attgagcagc
    73381 aaatgcctca ttagcttcaa ctaaatctat atcttcaata gtcatattag cagcttctaa
    73441 agctttttta gttgctggaa ctggtccata tcccattatt ttagggtcaa cgccagctgt
    73501 tccataagaa actatagttg caagaggctc tattcctagt tcttcagctt tttctttagc
    73561 cattactact agcatagcag caccatcatt tattcctgat gcattaccag cagtaactgt
    73621 tccatctttt ttaaatgcag gtcttaattt agcaagtttc tccattgtag taccaggctt
    73681 aatatattca tctttatcta ctacagtgtc accttttctt ccttttataa caacaggaac
    73741 tatttcttca tcaaattttc cttcagcttg agctttttca gctttatttt gacttgcaag
    73801 agctaattca tcttgttctt ctctagttat attccattgc tctgctatat tttcagcagt
    73861 aatacccatg tgatagttat taaatatgtc tgataatcca tcttttatca ttgaatcaac
    73921 aaaagcagca tcacccattc ttgcaccata tctcgcactt ggtactaaat aaggagacat
    73981 actcatattt tcagctccac caactaacat tatatcagca tcacccaatg ctataagctg
    74041 agatgccatt gaaacagatc ttaatccaga accacaaact atatttatag tcatagctgg
    74101 tttttctact ggtattcctg ctcctaatgc tatttgtctt gctatatttt gtccaagacc
    74161 tgctgtaagt actcccccta aaagagattc atctatcata tctggagtta tattagctct
    74221 ttttatagct tctttagctg ctgttacccc taactctacc gctgaaactg atttaaatgc
    74281 tcctccaaaa cttcctactg ctgttctagc tgcactggca attactactt ctctcatatt
    74341 cattaccccc attaaattag atttttcata tatccgaaat tgttttttta aacaatttta
    74401 tttaaatttt taattcccaa actcttattt attataatca tagaatccta tcttagtttt
    74461 tcttcctaat tgattagctc taaccatttt agctaaaagt ggatgaggtc tatatttagt
    74521 atctccaaac tcagtatata aaacattcat tatagctaaa acaacatcta atccgattaa
    74581 atcacctaat gctagtggtc ccattggatg gtttgctcct aatttcatag cttcatctat
    74641 ttcttcttta cttgcaacac catccgcata tataccaaca gcttcattta tcataggtat
    74701 aagtattcta tttactacaa atccaggaga ttcagataca tctactggta ctttattgat
    74761 actcttagat aattcaaata ctgtatcaaa agtaactttt gatgttaact gaccacttat
    74821 aacttcaact aatttcatca taggaactgg attgaagaaa tgcattccta taactttatc
    74881 tggtctctta gtagaagaag ctatttctgt tatagataat gatgaagtat ttgttgccaa
    74941 gatagtatct tctttacata attcatctag taacttaaaa acatctttct ttatattcat
    75001 atcttctaca gatgcttcta ttattaaatc catatctttt aaatcttcat agttagtagt
    75061 tgaactaaca tgacttaata tttctgcttt tgtagcctca tccatttttc ccttagtaac
    75121 taacttagtt aaatttttat ctaataaagc taaacattta tctatagcac cttgagttct
    75181 actctttaaa catacatcat gtccacaact tgcaaaagtt tgtacaatac cacttcccat
    75241 agttccacta cctattacag ctaatttcat agttataacc tcctgttatt ttagtattta
    75301 tattctgtta actagattaa aatactattc aatctagtta acagcaaata tatttttaat
    75361 tagattttta cccttttata aagttggctt ctctcttttc aacgaaagct gacattcctt
    75421 ctttttggtc tttagttgaa aaacaaagtc caaataaatt agattctata tctattccag
    75481 tatttatatc agtttgagca ccaagttgta ttgcttcttt agagtatcta actgcaagtt
    75541 gagcattttt agctattatc ttagctaatt tctcaacctc ttctattaaa atttctggct
    75601 caacgactct atttactagc cctatttttt cagcttcatc agcttttata acttgacctg
    75661 taaagattaa ttcttttgct tttgccattc caaccaatct tgtaagtctt tgagtccctc
    75721 catatcctgg agttattcca agagttactt ctggttgacc aaatttagct ttagcagatg
    75781 caattcttat atcacatgcc attgcaagtt cgcatcctcc acctaaagca aatccattta
    75841 cagcagctat cactactttt ttactatttt ctatctctcc aaaagctttt gctcctaata
    75901 tactaaaatc tttagcagct acagcatcta aatctttcat gtatgcaata tctgctccag
    75961 ctacaaatgc ctttccttcc cctgtcaata ttacaacatc aatagtttca tcattattaa
    76021 tatctacaaa tacttcataa agttcttcta aagtctttga atttattgca ttaagggctt
    76081 taggtctatt cattttaact gtacatatat ttccatttac ttcaacagct acattctcat
    76141 aaactttaac atcacttgta ctcataatac cctccccaag aaattagtta gctaaaactt
    76201 cacctttttc ttttgcaaca cttaactgac tgataagttc tggaagtact ttatgaacat
    76261 ctccaactat accaacatca gcatatttaa atattggagc ttctggattt ttatttatag
    76321 caactataaa ctcagcatct tccataccag ctatatgttg tattgctcca gatataccac
    76381 atgctatata aaggtctggt cttacagttt taccagtttg accaacttgt cttgctttat
    76441 ctaaccaacc tgcatctata gtggcacgag aaccagaaac ttctccacct ataatttcag
    76501 ctaattcata aagtatgtct aagttttctt ttccacccat tccacgtcca gcagaaacta
    76561 atatcttagc atcttctatt ttaacttgtt tcttagcttc ttttattact tctacaactt
    76621 gaactaattt atcagcatca ttaaattcta ccttgaaacg attaattaca gcttctttag
    76681 tttcgtcagg ttcatttttc ttcataactc ctggtctaac tgtagacatt tgaggtctga
    76741 aatctttaca aactattgtt gccattatat ttccaccaaa agcaggtctt gtcattaata
    76801 ataattttgt atcttcagct actgcaagac ctgtacagtc agcagtaaga cctgtatgta
    76861 ttctagcaga aactctaggt gctaaatctc tacctattga agttgcacca aataatacaa
    76921 ctatagggtc agctgctttt attgcttcat aagctgcttt tgtatatggt tcagttgtat
    76981 acactgctaa agcttcatca tctactacta ttacctcatc tgcaccataa tgtgctaatg
    77041 tatctattaa accttctacc ttactaccta aaagtaatgc agaaactttt gtatcatagt
    77101 cttttgctat ttctgtagcc tttcctagta attctaaaga aacagtttga attacatttt
    77161 ctctttgttc tattactact aaaacgttac ccattcttta tcccccctta aattatatga
    77221 tatacttctc ttttaattta tctatgataa ttccagctga tgttttcgca tcttcattgt
    77281 atattgtacc agctggttta actgattttg taaatgattt aaatacacta gttggagaac
    77341 ctttaagacc taaattagaa gggtcaacct ctatatcttt tacagtccat gtttctacta
    77401 catcattttc gaaagcatca tatattcttc caactttcat gtatcttggt gtgttcatat
    77461 ctttaagagt tgttataagg catggcattt taacttttaa gtcatggcaa caatcttcaa
    77521 attgtctttt tactaataca tattcacctt cagtttttat ttcttcagca tatgttattg
    77581 atggaagatt taaatgttca gctatttgag gtccaacttg tgcagtatct ccatctatcg
    77641 cctgtcttcc agctattata atatcaaaat ctatattttt taatgctcct gctaatgctg
    77701 atgaagttgc ccaagtatca gcacctgcaa atgctctatc tgttaataat attcctctat
    77761 ctgcacccat tgctaaagct tcttttaaag ccatatctgc ttgaggaggt cccattgtta
    77821 taacagttac atgagcaccc atttcttctt ttaattttat agcttcttct aaacctgctt
    77881 tatcatcagg gtttattata cttggtactc catctctaat taaagtacct gtattaggat
    77941 ctagtttaac ttctgttgta tctggaactt gttttataca aacgactata ttcataaatc
    78001 cttcctccat atctatttta atatcgacct attgtgtata ttttaatatt tatctatttt
    78061 aataattttc ctgaaataac cattctttga acttcactag ttccttcata tatttcagtt
    78121 atcttagcat ctctcatcat tctttctact ggatagtcac gagtgtatcc atatcctcca
    78181 tgaagttgta cagcttttgt agtaacttcc atagctgttt cagcggcaaa taattttgcc
    78241 attgctgctt ctactccata aggttttcct aagtctttat ttatagctgc ttgatataca
    78301 aggtgtctag ccgcctgtac cttaacttcc atgtcagcta attggaattg tgtattttgg
    78361 aattttgata atggtctacc aaattgtact ctttctttta catatttaac agtttcatca
    78421 agagcacctt gtgctaaacc taaagcttgt gcagctatac caattctacc accatcaaga
    78481 gtagacattg ctatcttaaa tccttgacct tcttttccaa gtaaattttc tttaggtatt
    78541 ctgcaatcct caaatattaa ctcactagta gctgaacctc ttatacccat tttcttttct
    78601 ttaactccaa agctaaatcc aggagttcct ttttcaacta taaatgctga tatgcctttg
    78661 ttccccttag atttatcagt cattgccatt actacatata tgtcaccagc tattgcattt
    78721 gttataaata tttttgagcc attaagtatg tattcatccc catctaaaac agcagttgtt
    78781 tgttggccag acgcatctgt accagcatta ggctcagtaa gaccaaatgc tcctaatttt
    78841 tctccacttg ctagtggtct taagaatttt tgtttttgtt cttcattacc atattgatat
    78901 ataggccatg aaccaagaga tgtatgagct gataatataa ctcctgtagt accacaaact
    78961 ctagacaatt cttcaactgc cattatatat cctacagtgt ctccaccttc tccaccatat
    79021 tcttttggat acggtatacc catcattcct gcttttgcca tcttttccac tgtttcataa
    79081 ggaaatcttt cttcttcatc aagttctgtt gctaaaggtt taacttcatt ttcagcgaag
    79141 cttacatata actctttaag catctgatat tttttagaat ttaaatccat aatagcctcc
    79201 tcagtatttt ataaatattt tattttcatt tattaattaa aatctcggca acatctataa
    79261 taaagtattt gaatatacgt cgagaaattt ataaccttaa tcaaactacg ctctatattt
    79321 atatgtgatt atactaacca attatgacat taatttaatc acaaattgtt aatttaaaat
    79381 caactacacc taaaaaaaat ctcgcttttt ggaattttgt gaaatttcaa gttaaaataa
    79441 gatttctttc acattgatta caaataaaat aatttgttta caatatttat atcagggtat
    79501 actaaatagt aaatattgtg tgtatagctg ttttttagcc atgtacagaa tatttttatc
    79561 gcataaaaat tattttaata aagctaaata atcatatttt tatctgacta ttttttattt
    79621 ttaaatatct tacatttata atagtatcac tcaaaggata atttttcaat aatttctaat
    79681 tattttttta acaatattta ttacattttt tccacttttt tgtcattttt ttattgtttc
    79741 gaaaaaaaat cagaattttt taactttatt ttggattatt agtttaaatt agattttcct
    79801 cttagattta taaatattta cacaaaaaac tacctctgca tagattgtat acagagatag
    79861 ttttttacta atttctacta tagtttacta tattttttta ccaattttta caaacatcta
    79921 cccatttttc acacttagaa aacttctcca tttcttcaca tgtaagttct attgcagagt
    79981 tactactacc acatgcagga aatactgtat caaatctctt cattgaatca tctaagtaca
    80041 cttttagaga attttttaaa ccaaatggac aaacaccacc aatagcatgt ccagtaaact
    80101 ccaagacttc ttctggtatc aacattttag ccttacaatt aaattcagcc ttatacttct
    80161 tattgtcaat cttagcatca ccagctgtca caattaaaat tgcatcatct cctattttga
    80221 atgagagtgt cttagctatt cttgctggaa taaccccagc tgcttcagct gcaagttcta
    80281 cagtagcact agattgttca aattctaata tactgtcgtc cttgccaaat tgtttaaaat
    80341 actctcttac attacttatt gacattaaat ctccctccta gtctgaactt ttataaattt
    80401 catattattc ttaattatat ctataattga tttatttttc aaattattcc ataatattag
    80461 cattccatgt atcattattt tttccaacta ctagatacag tccttttgcc attggaatat
    80521 taacaacata ttctccctta tattctacac tcataccaga ttcgttaaag tacctataat
    80581 caaaatttag tccttttgga attacaaaat caacataacc ttgattgttt ggttttattc
    80641 catacttcag agttctttca gttatagaat ttttttcatt aaataaatct atattcacat
    80701 atgaagaaca cttatttatt ccctttttat tttccacaca attttcaaac ttcattttag
    80761 cactgtttaa tttataacta tatttactat tattacttat tttaaatctt acaatagtac
    80821 caatatctct gcttctcctg ctatcaaaat caaaaacaat ttcctctaaa tgatttattt
    80881 cataatcttt gacacttacc atatctctat atactaattt atttatagct atgaatagtg
    80941 taaaaatccc aaaaataaaa aatgaaactt ttataaactt tttcatttct agtctcctta
    81001 tattttgtgc tattgtgcta taatattaat ttaaatttta ataattaaac ttattctttt
    81061 ttgaaatcct aatagaaaaa tacattgata ttataaaaat tattattgtt aaaaatgtca
    81121 tactatataa atttatatac ttagcattag ccatatcagt tacataattt gaaagatata
    81181 ctcctccaac tttactataa ttaaaatgct ttacttgata tacaatcata ctaatcatct
    81241 tcaaaagact tccatataca aaataaatta aaatacttac tataagacca caaactttca
    81301 tttttccata tccaaactta aaaattaaag gaagtaaaat cgtcaaaatt gctaaaaata
    81361 tatttacaga aaaaaataca tcatttaaaa ccatatttct aaagaatact aatgatgtca
    81421 atttactcat taaggacaca attattgtta cagaaattat tagtcctaca cctaataaat
    81481 attttgagta aactatatct tctatgctaa ctggcatact ccttataaaa atccttgaat
    81541 cattttcata atcacactca aacgtattta taaatatcag ataactaacg acaatactta
    81601 ctgtataata tgaaattgaa ttaaggaaaa aataaaacac aaaaaatagc aatatgtact
    81661 ttaaattacg gataccatct gaactaatcc ctattattaa atcttttctt attaaattga
    81721 acatcatctc acctcattta cataataata cattagttct tctatagtag gatttttgac
    81781 cttatgctta tcaatttcta tcaataggct ttttactttc aagctatctc caactttaac
    81841 aagtgcctca ttataatatt tatactcttt aacaccaact atactttctt taattgcttc
    81901 tagctctgct ttatttgccc taattatttt atattcttgt tctattaatt ctttcttaac
    81961 agtaaaaatc tgatttcctc tatttataaa aacaagataa tcagcaattt taattagatt
    82021 atcagtatta tgagtagaaa ttattacact actacttcca tttttaacaa aatcttgtaa
    82081 atactgcatt atttcactcc ttacaattgg gtctatccca gtagttggtt catccaaaat
    82141 caataactta gcattatgac taagtgcact tgataacata agcttctcag attgaccttt
    82201 agataaattt ttaatatgca tattttcctc tatatcaaac ttattaaggt attcatcaaa
    82261 tctttctcta tcaaactcct tatagaataa agaaactatt tttctaaatt ccttaacctt
    82321 taaatgtgga taaaattgta aagaatcata tacaaaacca atattgtttt taaaacttat
    82381 tggagaattt tcaatagttt cattacaaag ttcaatttct ccttcatctg catcaataat
    82441 ccccattata atttttatta aagtcgtttt tccagaacca tttggtccta taattccaac
    82501 aatatcccct tcctttaact ctaagtttat attatttaac ttgaattttc ctaatttttt
    82561 tgtgatattc cttatgctaa acacatcttc acctcttacg cattttttat acccacctat
    82621 acatatttta acacattcac tacaaaggtc atttataaat ttataaatta agaattataa
    82681 aactatgtta taatacaaag tatgaagagg tgaatatatg aaaagaatta taactataaa
    82741 tacaaaccaa ttaaataaat cccttgagac taaagaactc ttaactagaa aacttatcaa
    82801 tgctggcttt gaagtgtatt cagatattta tccagataca gagcttatta tatcaattgg
    82861 tggtgatggc tcttttttaa gaacagttag agatttcgat tttcctgaga ttcccataat
    82921 gggaataaat acagggcatc tgggtttttt tcctgatatt ttaccagata agattgatag
    82981 ttttattgaa gcttatacaa aaaaagatta tattattcaa gaaatgtcac ttcttaatgc
    83041 agaagtatat acaactacta gtggttctaa tatgcttgct gtaaatgagg ttgttataag
    83101 aggagacaag tctaggacta tacacctaaa tttaagcctt gataataaac atatacaaaa
    83161 ttttagtgga gatggtatga ttatttctac ttccacaggt tcaacagcct acaattattc
    83221 tgctggaggt agtattgttg atataaatct tgaacttatg cagattacac ctttacatcc
    83281 gataaacacc aatgcatata ggtgctttac atcaagcata atatgttcaa atgaatctgt
    83341 tataaaaatt gcaccagaat atagatttga agattctgta ttgattgtcg ttgatggagt
    83401 tgagcataga tttagacaaa tcgaaaacat aaaagttagt atttctgatg caaaaataaa
    83461 attacttaga atgtcaaact atgagttttg gcatagagta tctgaaaaat ttttatagac
    83521 tgtacttagg aggattttta atatgaaaat aggaataatg agtgatactc atggtagctt
    83581 actttacttt gaaaaagcgt taaatgtatt atctgattgt gatgttttat tacatggtgg
    83641 agatgtctta tatcatggac ctagaaatga tattccagaa ggatataatc ccaaaaaatt
    83701 tattgagaca ttaaacaagt tagaaaacat tgtgattgta aaaggaaact gtgatgcaga
    83761 tgttgaccag atggttatag aacaccctat tcagagtcct tatgtcatga gccaatttgg
    83821 tgaaattaga attatattga atcatggtta tacagaatca gaagaagaaa ttattgacaa
    83881 agcaaagaaa atgggtggtg acatattagt tttaggacat actcatgtaa aaaaattata
    83941 catggatgat aatttaatag taataaatcc aggaagtact tctatcccaa aagatggaag
    84001 ccattctgtc gcaattatag atatcataaa aactgatgat gaggatgaac ttgaacttga
    84061 tataaactta attgacatca atactgggaa tataataaat attaatgatt agtaaatttt
    84121 aaataaaaac aaagtaaact ttaatttatt aaattcataa taaaaaacaa gtaaaccgtt
    84181 ttatttctat ctcaattaga agtttaaggt attgtactta aatttctaat tgagatagaa
    84241 tctggtttac ttgttttatt gtatactata ttgaaatata acttagttta cttgttttta
    84301 ctatatatta tgctaaagta taacttagtt tacttatttt attatatatt cattttgatt
    84361 tacacaatat tactgtaatg ctaatgcctc tgcatatggt ttactattct tacctccacc
    84421 aacttgtaca aatttatcag cctttacttt ttttataaaa cttttctgtg cactacttaa
    84481 acttccactt gcaagtacta caggtgactt tgtttttgat gctaatactc cagcagcaag
    84541 accatcaact aaatcagctt ctctaccact tccatctttt gcaaaataca tattattaac
    84601 cttacttgat ttcccataaa acttttctaa aacttttgca ttagtatctt ttctatctgt
    84661 tcctgctagt ctttctgcat ttactaactg tccttcaagt tttttactta tactgtactc
    84721 accacctact atgtatgtct cagttgagtt ttgtgtaata aaattctttg cctcacttct
    84781 tatgctatct ttacctgtat atattatagc tgtattatca cttgccgctg gacttgatat
    84841 tgaagttgcg tctgccagtc cattataacc attaaccact gccacttttg agacacctaa
    84901 atcaccagta tcatctagct gtttagtaag aagtaaagat gtttcttctc tatctgctcc
    84961 accaattcta ctaactaata ctcccatatc ttcaagaaat tcttttacac ttgatgaaat
    85021 agctccttca cttccaatta aaataacttc tgctggatta agccttttta tttcctctgt
    85081 tacattatat ggtatactat ttttattggt aagtagtatt ggtgcatcca atttacttgc
    85141 caaaggagtt gaagccaatg catctggcaa tgaatttgag ttaactaaaa acaccctttc
    85201 tgaaccatta ttccatccac tttgagatat ttttatggct gtatcaaatc tatcttcacc
    85261 aataagtgtt tcctcctttg gagattttct gccttgaaga taattattca tatctacctc
    85321 tgtattttga tttttactaa tagtgtctgc atatacacaa cttggcatca taatcccaag
    85381 tgaaagtaca aacgctatta tttttctaga tttaattttc aatacaattc ccccttttaa
    85441 atgttaatac ctattaatat ccatactttt tattgatacc ctaaactaaa aataattatc
    85501 ttttataaaa ataatcataa aaatagctat atcatttcag atacaataca aacttatact
    85561 aagcttgact tataaagaat gatatagcta ttataacttt aaatgcttaa tttttaaata
    85621 ctttttaact aaactctttc agcatatttt tcagctattc ttacaattaa tttagtagct
    85681 ttctccatag aactaactgg tatacactca tttttcccat ggaagtttaa tcccccagta
    85741 aatatatttg gacaaggtaa tccattaaaa gataatcttg ctccatcagt accacctctt
    85801 acaggaacta aacgtggttt tatatttaat tcctccatag cttctttagc tatatcaact
    85861 ataaacataa ctggctcaat tttttctttc atattgtaat aagaatcttt taaatccaat
    85921 tccactctat tatcatattt cttatttatt tgttctatat tctctaccat aatagatttt
    85981 ctatattcaa atttttctct acaatggtct ctaataatat aagacatact tacattctca
    86041 acatttccat ctatagaatt taaatgccaa aatccttcat atccttctgt tgtttctggt
    86101 ctttcatctg ctggaaacat ttctgatatt tcagtagcta tatgtatagc atttaccatt
    86161 ttatttttag ctgaacctgg atgaacattt ctaccatgta tagtaatagt tgcatttgca
    86221 gcattaaagt tttcacattc aagttctcca actattcctc catctaaagt atatgcatat
    86281 tttgctccaa atttttcaac attaaataaa tcagcacctc taccaatttc ttcatctggt
    86341 gtaaatccta tttttatatc tccatgctta atttctggat gatttattag atattctatt
    86401 gcagtcacta tctcagctat accagcttta tcatcagagc caagtagagt tgttccatca
    86461 gtgactatta aatcgtcacc tttaaattct accaactcag gagaatcctt tacatacgta
    86521 ataacattta actcttcatt taaaactata tcttttccat cataattttc tattattctt
    86581 ggtttaatat cttttccact aacatcttca attgtatcta aatgtgatat gaatcctata
    86641 gtatccacac catcagtatt tccttttaaa gtagccatta tataactatt ttcatctaaa
    86701 ctaacatcat caacacccat tgtttttagc tcttcaacta tatacttagc taaaaccctt
    86761 tgaccttctg aagatggaca gttctcacac tgagaattag atgttgtatc aaatgatacg
    86821 tattttaaaa atctttctac tactttttgc ttcacgtata taccctcttt cttaataaga
    86881 ttaatgaaat tttttattta ctagtgaata aaattttatc ttttaaaaaa tttatcaatt
    86941 attataatta aaaaatatcc accttcttaa gtatatcatg catacatacc aaacccaaac
    87001 aaaaaattac tccaattatt ccttttactt ggattccaat aaaaatcgct gctagggtag
    87061 ctaatggatt tagtccaaca tttaccgata ctagtttagg ttcaagtatt tctctaatca
    87121 aagacaagac aaaaaatatc acagtcatgc ttatagctat aaaataattt tttataagaa
    87181 aataataaac aattactgga agatatattg ttattatacc tagaaatgga attaaatcca
    87241 tcaaagcccc cacaaatcct actattagac cgtatttcaa tccaaataaa gcaaaacttc
    87301 cccatattgc aaaaaatgtt atagccatga gtattgtata ggctcttata tatccaacaa
    87361 gtgaaagacc tgcctctttc tttacgtttt ttaccttctt tctgacatca acagtgaaca
    87421 tattgtaaaa actattttca atcttatcaa ggtcttttgc tataaagtat gtagcaataa
    87481 acaaagtaat tataaatatt ataatatatg gaatagaagt agccaatcca agtatactag
    87541 taagaagatt ttttgtaatt tgcattactt caccactgaa tttgtttact aattgttcta
    87601 tatcaaagtt ggctatttct tgaaatgact tcataaaatc atttatttta tttaaaaaat
    87661 acataatagt attacttata tcttcaggat tagcagatat attatttaaa aatcccatta
    87721 gttctttcac tgaatttatt actattattg tagtaactgt actgactata gccactgcaa
    87781 gaaaacttag aacaagcgta gagatacctt tatttatatg aaacttattt tctaactttt
    87841 gagatatagg attaatcata agtgctatta taccacctag aaaaaatgga gctatgtatg
    87901 gaaaagcttt gtaaataaaa ataaataaca atgtatatat acaaaaaaag atagtgttgt
    87961 tctttaattt atataaaaaa ttattttcta gtatgctcat tttcaaaatc tcctttcttc
    88021 taatttatat ttttattttg tccatttcat actagtttta tttgaatata aataaaaaga
    88081 agtatatgaa tataaaagcc aatttttata ttcatacact tctaataaat ttaagaacat
    88141 tattacatta tgtatatatt cttatttttt taattctatt tttttctata ctttctatac
    88201 aaaatcttat attatcgact tcaattactt cattttcttc tggcaatctg cttaggtgtc
    88261 caattataaa acctcctatg gaatcaaact cctcagattc tagatttaca ccaataagtt
    88321 cattaacatc accaatttta gtacttccat ctacgatata ctcatcttct tttataactt
    88381 gtatttcatc ctcttcttca tcatattcat cctctatatc tccaacaatt acttctacta
    88441 aatcttctat agttaaaagt ccagatgtac caccatactc atctacaact atagccatct
    88501 ggcttttttc aagtttcatt tcttcaagaa gttgtgttat ctttttgaac tcatacgtga
    88561 aaaaaggctc tctcatataa tttttaatat caaatgactc tatttcttca tcagataaaa
    88621 atataatatc ttttatgtta agtattccta caatatcatc tatagtttct tcatacacag
    88681 gcattctact taatttttca gttttaaaca cctgtattat ctcatcataa ctatcttcca
    88741 tatctatagc taccatatct attctttgta ccattgcatt tttggcttgc atatctccaa
    88801 attcaaacac attatttatg atttctctct cttccatttc aaggacgcct tcttcatgac
    88861 ttacatttac cattgttttt agttcttctt cagttatgaa cgatttagct cctttatttg
    88921 ttataccaaa taatttaaat ataatatttg taattatatt aaatatccac actattggtc
    88981 ttaatataaa tataattgct tttataggtt tggatactaa tagagaaacc ttttctgaat
    89041 tatttgctgc tattgttttt ggagtaatct caccaaatat taatactaat actgtcataa
    89101 cagctgtagc aagtgccact cctttttctc ccattaatcc tataaacaaa gatgttgata
    89161 tagatgttgc tgctatattt acgacgttat ttccaaccaa aatagaactt aaaagcttat
    89221 ttggattttc tattaatgaa cttactagct tagctccttt aactccttca tcttgcatat
    89281 atctgattct gattttactc aatgacatta aagccgtttc agatgctgag aaaaatgcag
    89341 atcctataag taatactatt aaaaatataa tctgaatcaa attatcgggc gattccaaca
    89401 cttaccacac tcctaattac acgtttttat attaatatta attataaata actttaatat
    89461 tagtttttat tatatcacaa ttacatatta tttggaaata ttagataata tattcaatta
    89521 acatatattt tctaactata ttcaattttt acttttgaac ctattccagc ctcactagat
    89581 gaaactaata gcttaacagg aactctattt ttaagttcct cgacatgact tattataccc
    89641 acacttagat tattactatg caatctttcc aaggattcta taacaatttc caaaagttca
    89701 ctatctaatg aaccaaagcc ttcatctaaa aagaaaaatt ctagtggtgc acttcccttt
    89761 aattgtattt gagatgaaag agcaagtgct aatgataaag atgttaaaaa tgtctctcca
    89821 ccagatagag tatcaatact tcttctttct cctccattaa agttatctct cattacaaag
    89881 tttagtgttg aatcaatttc aagtgcatat cttccttttg ttattccatc caatcttttt
    89941 gatgcttcca acgctatata ttttaattgg tttgtagaaa catattctac aaatttattt
    90001 ccttgtacac atttatctaa ttcttccaat aagtccacat tgtgttcaac tactttaagt
    90061 tgtttattaa aatcatttat cttatctaaa gagtctttta atgttattaa ctgattttga
    90121 ttggctccaa tatttttgga tatatttcct atttctactt tcaaattata aatttcatct
    90181 tttaactgtt caaaggactc cttctttaca cttcttccat ttaatttttc ttttgatgat
    90241 tttattttaa atgctaataa tttttgttct tcttcatgtt ccagtatttc ttcaatcaat
    90301 ttttttactt catcagcatc taataaagct ctttttactg catatatgct ttcaaattta
    90361 ttttctgcca acagtttatt tagtgttgat ttttggtctt tatattgttc cttagcagtt
    90421 ttaagtctac catctatatt gtgcttttca gcaatgtttt tttcatattc taatctctgc
    90481 tgttccaatt ttttcttgct tgattcctct tgctctaata tcttacatat atcttcttca
    90541 acactatgta aaagattttt agctaaatca ccttttgtta tgttaataac ttctttatac
    90601 ttattatctc tactcaattt tttttctaag tataattctc tagcctttat taactctatc
    90661 tctatttcat gtagcttact ttcattttct cttattttaa tatctagaga gtctctattt
    90721 tttaataaat ttgaatgacc aatacttagt aattctaatt ccttttcatt ctctcttatt
    90781 tcttttactt ttgacaatag attggagact ttagtaatcg tctttaatcc tatatactct
    90841 tgtttaagtt ttttacattt attttctaaa ccttctattt ctatgtttaa atctcttgtt
    90901 aatttcttat aattattcaa gctctctctt atttttaatt cttctttttc aattttattt
    90961 ttttcttctt tggctaatgt tactttattt tcggtactct ctttttcttt ctcccactcc
    91021 tgaatattag acttcaataa tgcaagcttt ctttgctcct catcaagttt ttttaataat
    91081 tgactagatg gaatatcccc cagttttcct tttacatctt caagttcttt agtcttcatt
    91141 ttttctatag ataaataccc acttaattta gcatttaatt cctctatatt gtttcttata
    91201 cttatttttt ctttttctaa tttctccagc ttttctttga caaaggatat attttcatca
    91261 tacttagtta tattatgatt ttcatgatgt tttgacccac aaactggaca aggcatattt
    91321 tcttttaatt cacgtctaag ttcagaggcc aaatttaaat actttagttg ttctaattct
    91381 ttttctaggt catctctatt ctttctatta ttttcaagtt tttcatttag taaatttact
    91441 tccctttcag tattaaactt actttcaagt attatcttta attcatcttg tatactactt
    91501 tttttgattt cattttcttt agtattatct gccttatttc ttaactcagt aacatattca
    91561 gattttaaaa gtaaatctgc actctttcca ggacattttt taagaagaac atccaagtga
    91621 agagataaat tttctaaatt acgatttaca tcattcttat ctttatcaat atatctaact
    91681 tttaagttta tatcttctgt ctctttgagt atttcttcta gtttatttat cttttgattt
    91741 ttttcttcca atacattatt gtaatctttc tcgtattcat atgctagaaa tattttttgt
    91801 tttaattctg cacttatatt tactttatta atctttcctt caacttcttt tatagattta
    91861 gtaactgcat cttttctgga ctctgacatc tgctttactt tttctaattc aactttagtt
    91921 ttatttaagt taatcccact ttcttttagc tcttttaact ctttatctaa taaaactaat
    91981 tcttcttcta attttattgc ctgttgtaat cttatttttt cttcacttaa tttaggaact
    92041 tcttcattct taattttgtt tattttttca tatctatttt ttataacttc aagttcctga
    92101 tttaatatta ctaatttctt ttctaaatta cttactgtaa agctatcttc atctatcttt
    92161 ttttctaaat tttgaactga acctatgtat gggtctacct ttttagcatt actatcattt
    92221 tctacaagag caatcttttc ttttatctca ccatttttca aatcaagctc attttttcta
    92281 agttcatttt tttctagctt taattgttct tcatatactg ttttactttc ttcgtaagaa
    92341 ttttgagcta ggtctaaagc attattctta tctttttcca aattttttaa ctctattaat
    92401 tcttgattta tattgttgta tacttcttct gtaattccat catattgact taattttgat
    92461 tttaaatctt tcaaactttg taactgtaaa ttttttctct ttttcacttt ttctccaaga
    92521 cttcttccat atttctcaag attaaaaatt ctctctaaca tatctcttct atctgcacgt
    92581 gataatctta gaaattcatt aaatttgtct tgtggtaaaa ctactgacct agtaaaatca
    92641 tttgcagtta gtcctacaac ttgagctact ttttcattta cttccacaac tttatcagcc
    92701 aaaacatttt gggtattgtc atttaatact tctattaatc tggcattaga ggtttttgtc
    92761 cctgttttac ttctagcaat ggttctatct acaatgtatc ttcttctagt gtttttactt
    92821 cctatctcaa actcatacga tataattgcc ttatcgcaca cactgtttat atattctttt
    92881 gtatttctgg atatatttcc atacatagca atagttattg catctaagat tgtagatttt
    92941 ccacttccag tagttccaaa tatcccaaac aaacctcttt caattaattt ttcaaagtct
    93001 ataacttgtt tatcaatata gctattgagc cctgtaagtt ctaatctaat tggtctcatc
    93061 tgctgattca ccttcttcac ttattatatc tagaaataaa tccataagtt ctccttttgg
    93121 ctctacacct ttactaaaag aataaaattc tctaaataac tctgccatag acttttcttt
    93181 tatatctaca ctttcttgtt cataacaaga tgttattatt ggctttattt ctattatgtc
    93241 ttttaatagt tctttcattt ttttaatttc attctgtgat attacctctt cagtatttat
    93301 ttcaaagtat gaccaaatct ctttatcttg attttgttca catatatcta gtgcttcttc
    93361 tattccatta catttaaata cttctatagg tttataatta ttaaaatata catcttcaat
    93421 tattggtttt tcaccagctt ttatatccac tatgtatgcc cctttggtat atgctctttc
    93481 atccttgcta tattgaagtg gagagccaga atagtatgca tttaaacgtt cagaagcttt
    93541 ttgttgtttg tgtaaatgac ctaatgcagt atattgagcc ttttctggca agtcttttct
    93601 ttcaaccaaa aaacttccac ctagttgtat aggtctttct gattcagtac tctctccacc
    93661 tattacaaat atatgtgata cagctatatt tatagtatca cttctaaaat tttcttctaa
    93721 acttctaaat atatctccta ccttttcaga ataatttttt tgtttctcaa aatcatcttc
    93781 acttgaaaaa acttcattta atcttttttc acttggataa ggtaatgtag caaccacaac
    93841 attttctcca tttatattca gttttgtaca tccctgagtt gcacatgtta tttcaaaccc
    93901 tttatactta gcttctatcg tagcacttaa tggataacca tatataagta tgccttgttc
    93961 atgtgctaga ggcgttattg cagaaagtct ctctggatta tcatgatttc ctgaaattat
    94021 taatacacac ctctgaccat tattagcaag tcttgataca gtctgataaa atagtttttc
    94081 tgcttgtgct ggaggattag aagtatcata tacatctcca gcaattataa ccatatctat
    94141 ttcatttgat tctactattt ttataaattc ctcacaaaac tttgcttgtt cctcaattct
    94201 agaatggcct tctagggatt tccctagatg ccaatctgat gtatgaataa atctcattgt
    94261 ttccccttcc tctatctttt aggtttaatt tatatataaa ctaataacaa acagcttcat
    94321 caactccaaa taaatatata catttttctt ttaccgactt accagttaaa gtttcaatga
    94381 tatctgcata caaatccaac tgttttttat atttctctat tatttgattt atattttctt
    94441 catttacaaa atctgtttta taatccacca aaactatttg attatcttct tcaaaataag
    94501 catcaactat ccctcttaac atgacacttt cttcatcata aagtttttta tcatcattgt
    94561 ttataagctt ttcgtaaata tatatgtctt tcatatttac ttgtgcgtaa attgattttt
    94621 ctctatttat aagttcagca ttcaacattc tttttcctat gttagattca aaaaacttat
    94681 atattttata tggatttact atactagcct gtttttctgt tattatacct tttgaaacaa
    94741 aacctcttat ttgtgatttt atatcattta cattacttac attttttaag tctaaaacct
    94801 ccataactaa atgaactatt gtacctcttt ctgctccact tattttatct ctttcttctt
    94861 cattttgaat aaatagaggc ttttttaata ttactttttg ttcaaatata gtatttatta
    94921 actcttcttc ataattattt tgtatctttt ttatttctgt aacagatata gtagcaggtt
    94981 ttcttgtgct aaattcatat ggatatatat agtttaattt ctcttctatt tcattgtaat
    95041 aatcactgtc aggactatct acatctattt tttcaagaat tttttcaata ctttctttct
    95101 cttcattatt cttcttttcc actaaaatat cattcttatc ccacaacttt ccataccatt
    95161 ttgaattatg ttcaacacta aaaactgcgt ctaatcctac ttcatctaat aaattagata
    95221 aatccctatg ttttaataca caaggcatta tccaatctaa aaaattttta ccttttaata
    95281 tctcatattg tgatattgta tctgaatttt caaccccatt tgaccatctc tttatactat
    95341 cttgtatatt tctagtagaa cctgttataa tcaatttttc tttagccctt gtaaatgcaa
    95401 catataaaac tctcatctct tccgataaat tttctatatt tattttgctt tttaacgctt
    95461 ctttagctat acttggaaaa gaaatccgtc tctcataatc tacaaactga ggtccatacc
    95521 ctaaattgtg atgatataaa atactctttt taaaatcttg agtattaaaa tttttaccca
    95581 ttgcagagca tataactact ggaaattcta gacctttact tttatgaata ctcattattc
    95641 taacaacatt tgcattctca ccaagtgttt ttgcacttcc catatcagaa ctagactttt
    95701 ttaatttctc tataaaattt acaaagttga atattccttt aagactagtc tcttcaaatt
    95761 gttttgctct ctcaaacaat acttttaaat ttgcttgtct ttgacttcca cctggtaatg
    95821 ctccaacata agcatagtat ccagtccttg tgtacaaata ccatataaat tcatctgtac
    95881 tcatgtacat tgacttttct ttaaattcct ttaacttaat taaaaaatct ttacttttgt
    95941 ttatgcactc ttcacttggt ataaattcag actcattttc attctgagaa tctttaaact
    96001 cattgtactc tgctgtactt tttaaaacct cataaaaaat tttatcttta ctctgaactc
    96061 taatatctat caaatcctct ggggtaaatc caaatatagg tgattttaat acagatataa
    96121 gaggtatatc ttgcattgga ttatctatta tttgaagtaa tgacaatatt gtttttattt
    96181 caatcgtatc aaaatacccc actccgacat cagcatatgt tggtatatcc atattcatca
    96241 actcatctgc aaacactgga gcccatgcag atgtagctct taaaagaata actatatctc
    96301 taaattccac aggtctatat ccacctattc ctttatcata gaccttttga agttttccct
    96361 cttcattaac tttcattaaa tctttgataa tctttccaac cattctagct tcaagttgga
    96421 tattatctaa tttttcttct tcctctattt cactttcttt gttattagtc ctatcgtctt
    96481 tatcgtttat aatatcatca tcaagtttat tatctttttg tattagatgt atctctgttg
    96541 ctccaccaat aatagatttc tcgtctgtat ccatctcaaa atttgcacct aaatttagtc
    96601 tttctttttc tgtatattca atctctccta tattctcatt cattatattt tcaaatatat
    96661 aatttacagc atctacaact tcttctctac ttctaaagtt tttataaagc attatttttc
    96721 tatgggaact tcccttttta tcattatagt tattatattt ttgcaaaaat aattctggct
    96781 ttgcttgtct aaatctatat atactctgtt ttacatcacc aaccataaat ctatttggag
    96841 tttctgtatt ggccacagct ttaagcagga cttcctgcac taaattacta tcctgatatt
    96901 catctataaa tatttcataa aactcattcc tatatccaac tgctatatca gaaggtacaa
    96961 tattcccttt ttcatctaca tcagtaagta tatttaaggc aaaatgctct atatcattaa
    97021 aatctattat tcctttttct cttttcttat tactatattc ctcttcaaat ctcagtacaa
    97081 tattagatat tggtttaact atgttataaa gatattttat ttcttctcta atagaatcat
    97141 tatctttatt gaatgtagca cttactatac tttctaaaga cttcttagtc ttatctctta
    97201 tggtttttgc cttttctttt gattctttta tatacgaggg agcatctttt gaaattcttt
    97261 ttactccttt tacataattt tcaaaggaca tacttgacat ctttttatat gtctcatccc
    97321 aagacttatt acatgcttgt gaaatgtcga ctattttttt atattcagca gataactttt
    97381 ctgcaaaagt ttctaattct tctattgatt ccacttcttt taatgccctt tccatgttta
    97441 gacaaagacc attaatctct atttttacag tgtctaatat agctctagcc caaatagact
    97501 gtgaaaaatc aaaattttca tcaatattaa atctttctgc tgagtcaatc aaccattttt
    97561 taggttcagg agaagccatt gcaaatgaat atattcccaa aataatatct tgtacttctt
    97621 tatctcctcc tctttctgca taactttcaa ctaaatttaa aaaaccctca tctctttctt
    97681 catataaatc ttcaaatact tcctctattg cttcttgttt caaaatagca cattcagttt
    97741 ggtctcctat tctgaaatta gggtctaagc ttattctatg aaagtttgat tttataacat
    97801 ccaaacagaa tgagtgtatt gttgtaatac tagatttatt taataaaaca agttgatttt
    97861 gtaaatgttt attttctggg ttttcatcta aggcttttcc tatggcatct cctatcctct
    97921 ctctcatttc tgatgcagca gcattagtaa atgtaacaac taataactta tctatatcaa
    97981 ttggattttc tctacttgtt atcatttgaa ttatacgctc aactagcaca gctgtttttc
    98041 ctgaaccagc agcagctgca accaatagat tacattctct actttctatg acctctagtt
    98101 gctcttttgt ccatttagga gaactcacta attaacatct cctttcatca tttttattat
    98161 ttcttcattg ctcttattat ttaaattttt atatttatta tctttcatag ttgaatcaaa
    98221 ttgacatata gcagaataat cacaaaagtc acatgatgtt ccatctttat gtttatatgg
    98281 agctatacat atttctccac ttaacatgtc ttcacacaaa tcctttatag catgctttac
    98341 atattttctg agtagtttaa aatcctcata gcttattgct gaagtacttt tgcctacatt
    98401 tccatcttta gtaagaccta ctggtattac taaagagttt tttctttctc catctattaa
    98461 tgatttatcc atctttttaa ctatgtgaga gtcttttact acaagcccct tcattctaag
    98521 ttcttttaat attgcttctt gtatttctac atcatcttta tcttcattaa attttgctat
    98581 tgggttattt atcctacaat atagtattgc agctggattt atatttatat ctttatcctt
    98641 tgcactttca aggattgcat ctaaatacac aagtaattgt aattgtagac cataatatat
    98701 ttctgttaga cttatggact tattgcctga cttataatct actattctta tatatttatt
    98761 ttctccttct tcaaattcat caactctatc tatttgtcca atcaaactta cttcttgtcc
    98821 attttctaac actattttta tagggggata ttttccattg tcaccaaatt taacttcata
    98881 atctattggt tcaaatgaac cttgttttat ttgctgacta attatagtta tagcagttgt
    98941 caacatattc tttaatctat atgcaaggta tttatacctc tcagaactat ttaatatata
    99001 tcctggtatc tttaaaatta tttcatcaac tattatacct atctttaatt ctatatattt
    99061 ttcatcaata cctctccaag ataaattatc ttgtaaaagt tcttttgaaa acctatctaa
    99121 aatattatga ataaaagttc ctaaatctgg tggtgtaaat gaatactctt tacgctttct
    99181 tgcttttaga ccatattgta taaaataagc aaatggacat tcagcatatt tttcaagcct
    99241 agatatacta agagaattac ttttatacaa tgaccttatt tttttctctt caatcctatg
    99301 aacttgatta gtataactaa gaccttttat aacttttttt gttatagaac tatagatttc
    99361 atcttttaaa tagtacctat atatatctaa ccaaatacta ttaatctctt ctttattgta
    99421 accatcactg tcataatttt ttattacatt tataagttca ttaaatgtag gagatttaac
    99481 cgttattttt ttaagtatat cttcatcagt gcttttattt tcttctacta aataactttt
    99541 attttctata tttggaaata tcttcttaag tcttgatata attattgatg gtctaagtgt
    99601 tttaccctca tggtccgata ttggatatgt tatggttaaa ttcttagatg tcgaagtaag
    99661 ggctttatat acaagaaact gttcttcaaa acttcttgtc tgactatcaa tatcgacttc
    99721 gattcctttg tttccaagac tttctctatc attatcactt aatattccac tatcctttgt
    99781 aatcagagga aatactccat cagtagttcc aactagatat aaatatttgg tatctggatt
    99841 tttcattcta tcaacactac ttacaagtac ttggtctata ctaggaggaa ctaaccctaa
    99901 ttcatattca tcaaaaccta aggttattaa ttttataaat ttttctaaag aaattatttc
    99961 atcacccata agttccacca tttggtctaa tatatccacc actatgtccc aaacttgtga
   100021 atattgattt gcaacatcaa gttctccttt atccttaaaa tttactatta aactttctat
   100081 agtttctggc atatttatat ctaataaaaa ttcatatata tacctacaaa tttcttttac
   100141 tctatttttt ccttttaatt tttcttgtaa agtaactatt ggctctagta ctctattttt
   100201 tatttcattt attttatctt ttaattccag ttcaaattca ctttcttcac ctgaaagact
   100261 ttgtgttatt ctataatccc atttttcatc aaaccatttc ttacctttaa tcccattagc
   100321 caagacataa ttttctaata cgtttatatc atcattatca attcctataa gtccactttt
   100381 taaataccta aacatagttt catacccata tcgtctattc ttcatttcta aagctgaaat
   100441 aattaaaata actattggat tactttttgc ctctctcttc ttatctataa agtttggtat
   100501 attatattca ttaaatatag agtgtactaa aaaatcatat ctatttaaat ctcttgtagc
   100561 aatagttata tccctatacc tgacattctt ctctctaact aaatgtacaa tttctctggc
   100621 tatttcttcc acttctgaat ataaattgtt aaactctttt atctttatat tttcagtttg
   100681 ctttgaatat attttatatg gataagcatt ataaaatctt tctaggtgct gtaattcctt
   100741 aaccttttca aatcttttta taacacctgt atttaaattt acttgtggca gtatttttat
   100801 tccttcttcg ttgcacaatt gagtcaattt tgaataagta aatttcgttc tagaaaacat
   100861 atcagcttta ctgtacccag tatagttgac atcatctaca gtcaaagaga tattaacact
   100921 tttagattta tttaatatac tctttatgac attgtactga tttggtgtaa aacctgtaaa
   100981 ttcatctata taaacgcaag ctccatctaa ataactgctt aattcaattt tactcgccaa
   101041 tgaagttaac atatcttgag catccacata attttcatgc aatttccctt caaaagagtt
   101101 atatatttta cttatatctt ttaattttaa acttaaagtt tcattatcca attctccaga
   101161 tatattttct aacatttcag ggcttatatt gtattgtttc atttctgata tcatctctgt
   101221 aattgagctt acaaaaccag cctgagattt tgattttgaa aacacattta attcttcact
   101281 tacatcttca atagccctat acaccatcat tgcttttcca ctagaattta tatttacatc
   101341 tgttaatcct cctacttgtg aaaaaactat attacttagt gtcttaaagc taagcatcct
   101401 aactcttaaa tacttatctt tttcttttcc taaaaatagt ttacttactc tattttcagc
   101461 ctcaaatgtg tactgttctg gaactaaaag tataattgat gtcgtttcat ttttttgagc
   101521 ttctttttta atctcatcta gtatataggt ggattttcca cttccacttc tacctaatac
   101581 aaacctaagt cccataaatt tccgcctcca aaatactata atctatttct ttgccattac
   101641 ataacttatt aaattcacaa tacttgcaat gctttttatt tctatagcac atacgttctt
   101701 ctttattata taccttttct atatcattgt atagattgtc ttcgtatgta tgttcttcat
   101761 aattagtgtt ttttatcata cttatataat tttgaatttt atttctattt gtttcatgtt
   101821 tttcttcatt atataaaatg gtaattggta agtcatcaaa ttcaggctga taatatctca
   101881 tatttatatc tttaacattt atgtccattt ttaaaacttt aggtattact tcttttgcta
   101941 aaaacatata aacaatagtt tgtattctat tttctacgtg tttatagtca atctttttag
   102001 actcggtttt ccaatcccat atatctatat tgttatttcc aattacaact aaatcatact
   102061 ttgcctgtat tgtatctcca tttaaggaca tccttacttc atattctggc aaatacgttt
   102121 ttcctttttc aataggaaca acttttttta tgttttccat ccactttaaa aactttttac
   102181 tatttttatt gtcctcatta aaatagattc caagaggtat attgctaaaa tatctttcac
   102241 aaatcaaatg aaaatctctt cctgttttta aagtttcata atactctcta ctaccaacat
   102301 catcattttt ccaatttatt ctatcaatat atttatattt aaatttcagt ggacatgatt
   102361 tatatgtgtt aattgaattt tgactataag aaaaatattt taatttatcg ttcactcata
   102421 tcacttctct cttaacttta ttttttaata gttacttact gtacatattg ttctatccca
   102481 ttttaaaagt atttcaagca atagtttaat aacttattat acattaattt gacctttttt
   102541 taataatatg tcgattaagt ctgcctaaat atatacatgg tttttgctct tttctcttat
   102601 tacctatatc actttcatct ctataagcac ttcctgaaag tactaacatc tcctttgctc
   102661 tagtaattcc tacatataaa agtctaatct tttcatttat agaatcaatc ttcgttttat
   102721 gagcataatc tgttgaaata cttcctttca atatagcttc gacttctgat ttaataattg
   102781 ccataggatt tttatatttt tcttttaaat accatttatc actctggaac ttttgattaa
   102841 tattgtcagg gaaattatat tcaacaagac ctagtaaaaa tacacaatcc cactccatcc
   102901 ccttcgactt atggtagtta catattgtta tactaccagg ctctggctca tatccattta
   102961 tctcatatac cacatctatt atatgattaa ataccttatt ctttacatca aacaataagt
   103021 tatatacatc ttctaaatcc atatttatat tttctgatgc tagatatttt atataaaagg
   103081 atatataatc aagtacagct ttatcttctt tttctaaatt aagttttttc ccaataaaca
   103141 aaactaagag gtctaacctt attattggat attctaatat ctgctttata atatttattc
   103201 ctcttttaaa atttaaatat atatcacttt cttcattaaa ttctattaaa tcattatcta
   103261 acttactaaa ttcaaaatca taaataattt cttcggtaga atagtttttt aatttttcta
   103321 aaaaatactt taattcttct tcattatcag tatctataaa aactttatca agtacaacta
   103381 ttaacttttc tatatcatca caatgtaaaa taaagtcaat aatagatgct atgttattta
   103441 taactctcct tttactcaaa gaatttggcc ctaattcttc aaagactaga ttgttctctc
   103501 taagttcttt agcaacttgt gtcacttgct cattaaatgg aaccaagata cctatacttt
   103561 tatctggata tttatttttt atacccttaa cgtatctagc agtttgttct atttcaaatt
   103621 tccaactatc ataccacttt atatttatat tataatgttc tggattagga ttttctttat
   103681 atcccattcc atttggcaca gtttttattt gcatatcttc aagagcatgt ctacactctt
   103741 cctgtctaaa ttcctttgta acatattcca caagagtgtt tgctaaatca agtatatctt
   103801 ttgaacttcg atttgacata tccatcctat aacagaaatc tgcactattt ataaattctt
   103861 taaaaaactt agggtctgat gatgaaaatg ttcctgttat actctgattt atatctccaa
   103921 ccctaactaa gtttctattt tcactagata taatttttat tatattacct tgaatttcat
   103981 ttgagtcctg acattcgtct tcaaatatat atttgtatct agtttgaaat ttctttctaa
   104041 gcccttcgtc taagcttaaa gttttttctg ctaaaattaa tatatcatca tagtctagta
   104101 atccattttg cttcaatttc ctatcatata atttatatat tggtaaaata attttaagca
   104161 ttcctttttg tcctcttgac aatatttctt caagtttatc tggagaaatt cccttatact
   104221 taagttcact tattgcattt cccgcaaatt caaaaaaacc tctttcccaa gcttcaagag
   104281 ttatttcttt ccaacgttca tctttttgct cctttaaaaa ccatctaaaa gctctttctc
   104341 caccttcaaa tctaaatcta cttatacatt catttaatat aatatttttt tgcaaatcgt
   104401 cagctatatt aaaatcctca cttaacatta caacttctgg cttttccttt attattttta
   104461 cagccaaact gtgtatagtc attacttcat acgaattagt atcatctatt ttatgctctt
   104521 caagaatctt tcttattcta cctttaaaat tatttacagc actattcata tacgtaagaa
   104581 ctaatatttt accacccttg tgcttctgtt caagtaatag tttagccact aaatttgtta
   104641 ttataaatgt ttttccagca cctggtactg ctggtactgc catagtacca ttatcgtaat
   104701 ttattattgg tatttggtct tctctataat ttatattaat cataattttc atctccttta
   104761 tgtgagattt ttaaaagtat actatataat atactctctt ggatataacc attaatcgca
   104821 tattcacttt tatatgcata gattttcttt gcatttaata gcaaattata gactgtattg
   104881 tatagataat atttcttata gtattcttcc atttcatttg tataaatttt tttttcttca
   104941 aaagattttc taagtacaat taaattactt atatcttttt ctattttcat attccaagca
   105001 ttacttccta tatctaccca tatttgtata cttctatcta tatttgatgc tatatatgaa
   105061 taaggagtag ttattactat tttgtctttg gatttcaatt cttctatatc gacaacagag
   105121 taataatcgt ttattgcagt ctttaaagct tctataaata tctcttcttt tttcattcca
   105181 agtaaactta aactttcact aaaagactca ctttcggcta ttatcttttt acataaccca
   105241 acattttttt taccatcttt taaacttagc attttatcta tataaaactg aattaaaaat
   105301 tcatgtattt ttaaatcact cttctttttt tcttcaatat actgtaaaat atcacgatac
   105361 tgactatgtt catccatatt atcaatactt aaatgtcttg tttcattaaa aatcttaaat
   105421 gcttttatcc tatttatttc taaaagtgtt tctaaaaagc ttaagtattc atcatccttt
   105481 ataaaatcta aatatccata gaacatgcat gtagccacta ccaatgcatt tccatatgga
   105541 taatctacag cttttttatc tttcttaata ttaaaaacat ctatattttt ttctatcaaa
   105601 gaatctctaa tttgatactc caatatacta ttgtttatag gagatataat tgctatatct
   105661 cttagagata caccttcttc tataagagat atgatttttt cacttattaa gtctaacatt
   105721 tcattgtata attgacttga ttgatttaat tttatagttg caggtaataa gtataaatct
   105781 tctattccta tctccttcct tttaatattt tcttctttgg acttaatttt tgatattatc
   105841 ttctcattta tatagtccat atccacatta ttaaatacag aataatccct actatagtca
   105901 aaatatatat aactttgtaa tgtattatct aatgcttctg ttataaaatc aacttctgca
   105961 ttacttgagc tttcaaggct atctactatt atatatctaa cttctttaaa taagcttttt
   106021 acataaaaat catcattaag tagatactga ttgtataaat atacacttaa tgaattatct
   106081 aaaatggaat ttgatagtaa tgaatttata taatatgata taatttcatc catctgagtg
   106141 tatgaaaatc tatatatact atctttattt ttctttgatg aatatatttt ttctccaata
   106201 aaatcaaaat caattaaatt aaatgctgac ttatttatat tagtcattat actctttgct
   106261 attgatttat ttgttccagt aatgtcttca aaatatcctt cttgtatcct tttttcctta
   106321 actttttgca ctattaaata ttcacttaaa ctatttgata taaaggttgg agatacagcc
   106381 tttttattta tttcatcaca attttctatt attagtggcc aatatttcaa taattctttc
   106441 ttcacaaaac ttatatatgt tgttattttc agttcttcac tataatttaa tcttaaagac
   106501 ctttcatatt gtatctttgt gttgttgttt ggtactaata aaagaatact tcttgaatta
   106561 ttcttagttt tatctaatat ttctctatat ttatctaaag gttcatttcc acattctttt
   106621 aaactacttt tgtcaattaa atattccaca taatcacctt cctcaattaa attaagtgta
   106681 ttttatctaa ttatatcata ttgtgtaatt tacaaaatac aatcaaatta ttgcatttaa
   106741 ttacaaaaaa agactatata tacattcttg cattcatata tgcaaatgta catacagtct
   106801 ctactattga tacacttata tttcatttta taactttagt ataaatttac attaagtttt
   106861 taaataattt ttatttctat ttattttaat gaatcttcta ttcctttgcc tatttgtata
   106921 cccatttctt ttactttcgc aggtgagttt attaaactca catctttctc attatccaaa
   106981 aatgcacatt ctacaagtac agctggtata ttattagttc ttaaaacgta taaataatct
   107041 tttgtagagt cactaggaag tactcttgtt tttatgcctc tgtttgtaag tttaaatttc
   107101 tctaaaattc tatttaatat gtttgtagct acagtcttag tagttcctcc atttttatct
   107161 ttaaacttat aaaagacttc tactccatga ccttttctat tattatcacc attgtaatgt
   107221 atacttgtaa ataagtctgg tttaagtgaa ttagataaag cagttctact tccaagagat
   107281 aaggttttat ctgtatctct agtcataatt acatttatat cctttgaacg taaatattca
   107341 gtagttgcaa gtgaagtatt taaattgtaa tctttttcaa tcattcctcc aaatgaattt
   107401 ccaggtgctg aaccaccatg ccctgggtca atcattactg tcttagcacc attattactt
   107461 cctgggtctg ctggagttgt attatgttta gataatgatg atgctataga actcattacc
   107521 ttagaagtca atcctccacc tattttatat accttattag ctgatttagt ttctaaattc
   107581 gttttatgat atgcagatac atacgtcttt ggtgttataa gtattggcga ttttaaacta
   107641 gcagccaatg gacctgctgc taaagcatca acaagtacat ctgatttagc tactaaaata
   107701 gcttctaact catcatctgt atagaatttt tttattacat ttgcatttgt ttcatgtcta
   107761 tctgctccat acaccctatt attagtaacg ttatccttag ttattccatt taccttgttt
   107821 ataacatttg ttgatatcat ttgaggacca cctataaaat aagcattttg taaatcctca
   107881 ttctctagcc atttagacgt attatctgta atactatctt tatcagttag tattattggt
   107941 tgcttatctt gacctgcttt tgctgctatc gtaagtgcat ctacttctcc accattagca
   108001 tttgttatgt atactttttc cacatcatga tttttatcta tttcctttgc tatcaataaa
   108061 gatgtctcat atctattaga gccatttaga cgtgtttgag tagcatttat agttgattta
   108121 atctgattag cagtagtttt agaaataga
//
LOCUS       DAMJVM010000003        88673 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.003, whole genome shotgun sequence.
ACCESSION   DAMJVM010000003 DAMJVM010000000
VERSION     DAMJVM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 88673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88673
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.003"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            141..770
                     /locus_tag="PC523_000228"
     CDS             141..770
                     /locus_tag="PC523_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473800.1"
                     /translation="MNKYLLINPVAGRMYGEKFHLVKENLMKKGYIIAECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESINLLKRNNLINSFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLIVTCPCTQLRDFASEKFKDKSNAIFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGSEDKYDIVEMFYCKDGCNNGDGL"
     gene            771..1553
                     /locus_tag="PC523_000229"
     CDS             771..1553
                     /locus_tag="PC523_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2473801.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGIPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVDAYE"
     gene            1546..2424
                     /locus_tag="PC523_000230"
     CDS             1546..2424
                     /locus_tag="PC523_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2473802.1"
                     /translation="MNNLVCDSGYIIRNLSKSYEVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLENINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     TFHNQRKNTILNKMLRKFKHNKRVIGKIINDNFRSLETNENLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLFMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKIKEFNIEDDYNRDLTSEYYI
                     KLKKDILMTMKSNKGI"
     gene            2457..3416
                     /locus_tag="PC523_000231"
     CDS             2457..3416
                     /locus_tag="PC523_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2473803.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSIDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYV
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            3730..4422
                     /locus_tag="PC523_000232"
     CDS             3730..4422
                     /locus_tag="PC523_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2473804.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSNIRRVYGEYSTKIDERIVELDGLKFYPERLELELHSKNLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     IRGLGYKLNKNW"
     gene            4432..5436
                     /locus_tag="PC523_000233"
     CDS             4432..5436
                     /locus_tag="PC523_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2473805.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVLTIIYCNLMKFISFDESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGIHSLEESVLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHGEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIREEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQIDKFTYVYTDV
                     KWMKFIIGQLVTNGVKYSKDCSNYITIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSISFNKDVNSLIKM
                     "
     gene            5672..6523
                     /locus_tag="PC523_000234"
     CDS             5672..6523
                     /locus_tag="PC523_000234"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDF2473806.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVREFLKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSVMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAIYRIIMGAVLAVLA
                     LTNIISI"
     gene            6657..7403
                     /locus_tag="PC523_000235"
     CDS             6657..7403
                     /locus_tag="PC523_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2473807.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKDKNLLLLKGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDRRLNEVSEILGIKDLLGKRTFEVSGGQAQRTAIARALINR
                     PSILLADEPTGNLDSKSSKTVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     AIYNEIYKGSSREQFYQDIMDVLTLLGGDN"
     gene            7403..9319
                     /locus_tag="PC523_000236"
     CDS             7403..9319
                     /locus_tag="PC523_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2473808.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASITISSSLLFSFVMFTSHPDL
                     DLSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISNRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSLFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAVTST
                     DQYTTYLRLVPVTSLTIAATYLFFSQFSVFFIKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVKRQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMRFIENNLKKEDIDYTRINGEFKTVFPKKSDFNVRIMKESKYLEITEVIG
                     VKPISFEDNESISLLSSSLPGNKKIKENVTVGNRSLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFDNESGMQPYLFFSKELMIET
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLLPYVVAVIHSIFALTALKNSFDLEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIKLNLVNY"
     gene            9598..10515
                     /locus_tag="PC523_000237"
     CDS             9598..10515
                     /locus_tag="PC523_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2473809.1"
                     /translation="MMILIQSRGKMKCKELAEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKTEFNSIVEKIKAVLN
                     TRVKSDTYMDYFTIQPQRNCDYKFEKNKCNEIIRAYTTKRKLWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKNYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEINVVIKISHPFSTIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVDPDSLRNDILSEIEKMKKIY"
     gene            10593..11330
                     /gene="cas6"
                     /locus_tag="PC523_000238"
     CDS             10593..11330
                     /gene="cas6"
                     /locus_tag="PC523_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF2473810.1"
                     /translation="MKMKIEFSTSCIPISYNSLFMSIIKEAIKKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYVKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KDYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGSGLKRKLEFENIDLKKVVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNIIYNLGLGFRRAQGFGDVDILEWR"
     gene            11336..13186
                     /gene="cas8a1"
                     /locus_tag="PC523_000239"
     CDS             11336..13186
                     /gene="cas8a1"
                     /locus_tag="PC523_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HDF2473811.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKSNKQAEIFIMEDYIEFDSS
                     FLENFHEYYFNYFMDEYDVSKRIKKSIDYSINFIKSKPDKVKDGIKKIKDTVKQQNDK
                     VKKFDEEKFNLIKEKLDSISKIKSYDEFDSLEILVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQVMFNDYLRQIIYFGELEDLLEENDYDKLKNY
                     LNDRLNSIAKDISEKKVSKSSVNTIEKIMKEINKNFIKKNKSIENIKEYMDSLEICEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWKQEYVSSICDVCKLILFCTPAGATYTR
                     KDYLTNEENEFYLFVNMDTSINELFDRNNSLKAQKSESSNSKDENPFNQLIKSIVEEN
                     KLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFVKENKKIQSIYDYKLKSN
                     VLDLILKSKDLKHLINNTLRNIVKSNLESNNKSNISGLSCFNVVQLRTILNNYKRGVY
                     KMDYKKLRKIYESGCEIHDYYIDKNAKNKIEGITYKILNLIKVGNKNDFMQTILRIFS
                     PSEKTPPEEILQIFVEEDLDFESVGYAFVTGLISGKYEGKDKLSNEKEEK"
     gene            13188..14102
                     /gene="cas7i"
                     /locus_tag="PC523_000240"
     CDS             13188..14102
                     /gene="cas7i"
                     /locus_tag="PC523_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HDF2473812.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRADGNIYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREAAVRLSNAISLEPYRSDMDFLNNKGLADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGEIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVVQGTFLGKYLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            14104..14805
                     /gene="cas5b"
                     /locus_tag="PC523_000241"
     CDS             14104..14805
                     /gene="cas5b"
                     /locus_tag="PC523_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HDF2473813.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LKEIYDSLLNYNEHLSLGRKEDLVRITDIRFVEVNKFEIDEDIQLDDYENYKLSECDI
                     RMPIYIPKTSLQDKDIDGISYRLNNYYDNNAVGDKKRVWNKVDSYYVEDGNTISVGDI
                     LLDNEGDLLYFNNIN"
     gene            14816..17137
                     /locus_tag="PC523_000242"
     CDS             14816..17137
                     /locus_tag="PC523_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF2473814.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGTNINSLNFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIISRMDTKINEPKIVTHLENNVGHNYLSPAFIDY
                     SYIDKKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQHEFKV
                     RYPIKTEKLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEVVECNNVMN
                     IGEHTENYLMKEFGSLRETQSFAQSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIVGVGESNDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGKFMIMTATMPTIYIDELKKRGVVDSNLANLTCNTEKIRHCVSIVENSIDENLEQII
                     QSGINKKVLVIVNTVKSAIEKYELIEEIIKSKGIDINVNLLHSMYIQEDRAKLEKYIK
                     EFADSDSNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKNCMNGKKCFKMKEKHKVQMVKILYSKESLKD
                     TDFEKKFTRALDILDTITPYSLGSNEAQNILRNIEGYTVIPEDIYRKIEKTLIRDYEE
                     LGQELTEAYSNDDRQLINEIKSKRKKARREIIKKTVNLPIYKAKQNIVDIQVKGLEDL
                     KILLYKYDIHEDVHTKHIFGKGVIIGKEIDQFI"
     gene            17152..17703
                     /gene="cas4"
                     /locus_tag="PC523_000243"
     CDS             17152..17703
                     /gene="cas4"
                     /locus_tag="PC523_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HDF2473815.1"
                     /translation="MWLKFTKEGEFVPIDLDLLKVQGVKINYYYVCKRKLWLFSKGIT
                     MEQNSSRVLSGKIVHEDSYKRMKNKEMLIDDILKLDIIKDEYVKEVKISSKMKEADEM
                     QLYYYLYYLKNSMGIEKKGTINYVKEKKQEELILTEEKERIIENTLININEINMKLSP
                     PKIEKKPYCTKCAYYEFCFVKEE"
     gene            17706..18686
                     /gene="cas1b"
                     /locus_tag="PC523_000244"
     CDS             17706..18686
                     /gene="cas1b"
                     /locus_tag="PC523_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HDF2473816.1"
                     /translation="MYKDYYIISNGNIKRKENTIYYYNLEGDKKILPVEQVDDIHIYG
                     EVDLNTKLINYISQYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDAIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNALMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIIDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            18689..18955
                     /gene="cas2"
                     /locus_tag="PC523_000245"
     CDS             18689..18955
                     /gene="cas2"
                     /locus_tag="PC523_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HDF2473817.1"
                     /translation="MFVIVTYDIVEAKSLNRIRKILRKYLTWTQNSVFEGNITDGKLH
                     KCISEIENIIDNREDSIYIYEIKNPNSIKKKCYGIDKYSDEMFI"
     repeat_region   19139..21260
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=19139..19167
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaag"
     gene            21321..21749
                     /locus_tag="PC523_000246"
     CDS             21321..21749
                     /locus_tag="PC523_000246"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDF2473818.1"
                     /translation="MIRKLNNKDINKIMEIWEESTIKAHGFISKEYWQNNHNAVKNEY
                     IPISDTFVYDIGDEIKGFISIIDKSFIGALFIKPKYQNLGIGGKLLDYATEKYKSLSL
                     AVYKDNKKAVIFYNKKGFNVVKGQLNEDSGFKEYIMEYSK"
     gene            21885..22052
                     /locus_tag="PC523_000247"
     CDS             21885..22052
                     /locus_tag="PC523_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473819.1"
                     /translation="MVTKEENKIVWCISNLTNLPFKNNKFDIILNVLSPVNYKRFERV
                     LNNYGMARKVC"
     gene            complement(22212..22982)
                     /locus_tag="PC523_000248"
     CDS             complement(22212..22982)
                     /locus_tag="PC523_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473820.1"
                     /translation="MKVNENNIDEELDELSRKVYLESPDFWISISDKIIDKVFDEMLL
                     FFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYLLNL
                     NKDNNLDERPILDELNYNTSSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDITYKF
                     SSKEAEPIEISNEVSSIRCCNCDELLEDVTLDKLSNLCKIQNCNNCGSNLTKVGIIAK
                     KKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
     gene            23306..23851
                     /locus_tag="PC523_000249"
     CDS             23306..23851
                     /locus_tag="PC523_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDF2473821.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRKNL"
     gene            23872..25008
                     /locus_tag="PC523_000250"
     CDS             23872..25008
                     /locus_tag="PC523_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDF2473822.1"
                     /translation="MEEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKHLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKIKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNINNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIKKAELDFWVKESHKRAKEAREEKIFDDIIFPINNSTCDEGIRDKMSQRLLDRMP
                     SILGKESITNAANSCLINDGASFIILCSKKYLEHAKNKPIAKIVNTCTIGTDANLSPT
                     SAIKAMDKLLEIESLSYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALMIERV"
     gene            25012..26403
                     /locus_tag="PC523_000251"
     CDS             25012..26403
                     /locus_tag="PC523_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDF2473823.1"
                     /translation="MNYYELLRIKSDRHKNKNFLIIDGEKYTYENILSYSEEIGKQIY
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNLSIFNPKFNIYMYQYKKLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNAIFNISGESILFINGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRTIKQYNVTNIYLIPIKLQLLVKHLKESVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIERPLSVGRPFPGIDVYIREEKIFVNT
                     EYAVYNATKPYSVNDIGYFDNDGYLIFEGRSDDIVNIGGFKVSSTKIENEVKKIPQIE
                     DAVVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            26479..27354
                     /gene="dpsA"
                     /locus_tag="PC523_000252"
     CDS             26479..27354
                     /gene="dpsA"
                     /locus_tag="PC523_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HDF2473824.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDNCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAISEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTASAFGYSSISLNQLDEKLLQFDYIFNTI
                     PDCILTKERLEKVSQEVTIIDIASSPGGVDYSATKEVGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            27357..27947
                     /locus_tag="PC523_000253"
     CDS             27357..27947
                     /locus_tag="PC523_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HDF2473825.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEDAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(28154..28354)
                     /locus_tag="PC523_000254"
     CDS             complement(28154..28354)
                     /locus_tag="PC523_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473826.1"
                     /translation="MKKLKIISIFSLIISVILTIGGIGIVTYYVNNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
     gene            28858..31512
                     /gene="adhE"
                     /locus_tag="PC523_000255"
                     /gene_synonym="adhC"
     CDS             28858..31512
                     /gene="adhE"
                     /locus_tag="PC523_000255"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDF2473827.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIKEAQKI
                     FATYTQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDESADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMDKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKHTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     VASFLGLKGNSEEEKFQSLLSAIEDLKFKVGIPKTIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
     gene            31788..34007
                     /locus_tag="PC523_000256"
     CDS             31788..34007
                     /locus_tag="PC523_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2473828.1"
                     /translation="MTRRSKSFTLIIILFLTSLFLTMGYIYIEDTKNLLMSEAKIHIK
                     EVAIQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDELENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYADEVNKSIKKITISVPLLNNDK
                     VVGVLYCTYNINTLMKLINVSFYENNSISYVVKNDGTVILHPQEDSISKNIYKLLKQD
                     NDIREVNSLKKELQENKTGATVLKMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSEKIINRAIYAVLFIILIFIAIIFYIIYIKKSNEKEILSLAYEDKVTHIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVFARLSSDYFAIFCDYKNGRNKIIRKLDSIRNNIESNLSIVFEISLCVGIYFVEED
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVNGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFEKVCTD
                     LSEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIQMYNLSSDLIEIEITETVAINE
                     LNILKNVLNELRKYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLDGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            34261..34923
                     /locus_tag="PC523_000257"
     CDS             34261..34923
                     /locus_tag="PC523_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473829.1"
                     /translation="MIDVSIDKKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TLNKNLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSEESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            35293..36702
                     /locus_tag="PC523_000258"
     CDS             35293..36702
                     /locus_tag="PC523_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDF2473830.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGILFVAYIGMAIFFRNHFYF
                     GTTINGVRASGKTVEQVNDLLTSSTNSYTLNLDERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMVTLSYNKDLLEKRFNALSCLDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEVPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKNITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNRDDIGNTYVEISLSNQHMWFFKNGEMLVSTDIVTGNLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGGIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            36817..37092
                     /locus_tag="PC523_000259"
     CDS             36817..37092
                     /locus_tag="PC523_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473831.1"
                     /translation="MNEKLTKILEKAKNDDEFRKELIKSREQRDVMDSFCKVVTSRGY
                     DITVGELFSIGEEYTSNLLKSVNGGATYPIEDWSDWYEDFFIMLSNI"
     gene            37722..38033
                     /locus_tag="PC523_000260"
     CDS             37722..38033
                     /locus_tag="PC523_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473832.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAKERKSKAIEAVIGKVV
                     R"
     gene            38035..39960
                     /locus_tag="PC523_000261"
     CDS             38035..39960
                     /locus_tag="PC523_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDF2473833.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEEQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDGLRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHAESIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIALVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGVLGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            39963..40454
                     /locus_tag="PC523_000262"
     CDS             39963..40454
                     /locus_tag="PC523_000262"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDF2473834.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            40506..41069
                     /locus_tag="PC523_000263"
     CDS             40506..41069
                     /locus_tag="PC523_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HDF2473835.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            41084..42061
                     /locus_tag="PC523_000264"
     CDS             41084..42061
                     /locus_tag="PC523_000264"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HDF2473836.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKVMSSAILEAFDVYSKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            42048..42371
                     /locus_tag="PC523_000265"
     CDS             42048..42371
                     /locus_tag="PC523_000265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDF2473837.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            42488..44266
                     /locus_tag="PC523_000266"
     CDS             42488..44266
                     /locus_tag="PC523_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDF2473838.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTIKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            44263..45636
                     /locus_tag="PC523_000267"
     CDS             44263..45636
                     /locus_tag="PC523_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDF2473839.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            45642..46310
                     /locus_tag="PC523_000268"
     CDS             45642..46310
                     /locus_tag="PC523_000268"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDF2473840.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDAAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSAAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            46587..47636
                     /locus_tag="PC523_000269"
     CDS             46587..47636
                     /locus_tag="PC523_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2473841.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADVIWGLSAMNMIELKE
                     DGALEAYKPKHYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGAKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPVEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYSHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLSSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            47732..48721
                     /locus_tag="PC523_000270"
     CDS             47732..48721
                     /locus_tag="PC523_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2473842.1"
                     /translation="MSYLKIKNVFKNYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGDIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKISKEKIDNKVKEVLEIVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            48726..50444
                     /locus_tag="PC523_000271"
     CDS             48726..50444
                     /locus_tag="PC523_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF2473843.1"
                     /translation="MNNNAIGYKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASETLLNSLKNTIFVSVISTVISLALALVYAYALTR
                     TNLKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGIHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLNSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGIVGIIAFGIISVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFDFMDTIYLKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILITNILVKAIHEFIKNRLE
                     KRTNFN"
     gene            50872..51603
                     /locus_tag="PC523_000272"
     CDS             50872..51603
                     /locus_tag="PC523_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HDF2473844.1"
                     /translation="MGRAKYYDIYIDLKNRIENKEYLYQDLLPSEYTLIEEYNCSRNT
                     IRRAIQELANQGYVQSKHGKGVFIIYKANKQSEFLLGGTESFKESAIRNKKEYKTKVV
                     HFEEMVIDDKKSQITNFEVGTEVYYIQRVRILENKPSIIDHNYFMKDIVKDINEEIAS
                     NSIYEYMESELNETIVTTKRTMTVEKATKLDKKYLELGDYNCVAVVSNNTYNAQGIMF
                     EYTQSRHIPDGFIFFHQSQRINNIK"
     gene            complement(51619..53376)
                     /locus_tag="PC523_000273"
     CDS             complement(51619..53376)
                     /locus_tag="PC523_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473845.1"
                     /translation="MKGLYQMNIKEIPFSYFGSYMSISYSDELGFTLKSLRGKSKKNM
                     DVLRIIPTVNNMPVDYEFEANYFSILIKFNNGSISICFEGDSKIVFYGKGKNVGLKLD
                     TLPIYNFEYNYLMGKKGSEYCVINSYKNLTKFLVFSVNGKVGLSQNIFIDDCASTNKA
                     NNTSIIDVKSNSDNEFICVIEDIPTHMKVPDYKEYNFETSLNSTKKLFDEYYLAQPKV
                     DEKYKNSLLKASYINWSSFVKPQGYLKRYTILASNSTFLGAWSWDHCFNALALAGVND
                     KLAFDQMAVLFDYQDEYGQIPGSLSDSTIRWNFTKPPIQGLFFSKMMKKINFTKETLE
                     KIYNYIEKQVLFYVKFKDSNMDGIFEYHHGNDSGQDNSTIFKGAMVVDTPDLTAFIIN
                     AMDMLSKVAEKLNRNSEKEYWSNKADELTKLFLNYFIVDDLPVARETVTGEILSNNAL
                     LPLVSLVLGNRLPKYIRDKMIDLVTSERYLTKWGIATEAIDSDEYQDDAYWRGPIWAP
                     TTLLFVEALEECGENELAIDIAKRYCELVDKNGFAENFNAITGEGLRDKSFTWTASTF
                     IYLAAKVYEYNSQRINNIK"
     gene            53606..55039
                     /gene="treB"
                     /locus_tag="PC523_000274"
     CDS             53606..55039
                     /gene="treB"
                     /locus_tag="PC523_000274"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HDF2473846.1"
                     /translation="MRKNKILNSKESISKIIEYVGGKENIQSATHCVTRLRLILKDTS
                     KANISKLEDIDIVKGCFNAGGQFQIVIGTGVDKVYKDLIDQTGAEEVSKEEIKQQVNE
                     KSNKFQKFIRILADIFIPILPAIVASGLLMGVNNMIANPGIFYEKSVIEVNTDIAGIS
                     GIINLIANTSFTFLPALIGWSAAKKFGGNPLLGVVLGLVLVHPALMSAYDFAANPSAA
                     PTWDIFGMKVAQVGYQGQVIPVLIATYILAKLELFLKKTVPDAVQLVLVSPIALLITG
                     MLTFVVIGPITMTGANMITNGVVYLFEVAPVFAGALYSFISPPLVITGMHHLFLGVNL
                     QMAGTLGYVTLWPISETVTLAQGAATLAMFFILKNNKNLRGVSITSTISAWLGITEPA
                     IYGINLRFRYPFIAVMVASAIGGAFISYHNVKATAVGVGGVFSFLSVMPQYWGVYFTG
                     MAITFVITIGLTYLFYKSGKFKTNDNI"
     gene            55102..56760
                     /gene="treC"
                     /locus_tag="PC523_000275"
     CDS             55102..56760
                     /gene="treC"
                     /locus_tag="PC523_000275"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDF2473847.1"
                     /translation="MLDFKKSIVYQIYLKSFNDTTNNGLGDLNGITEKLDYLEFLGVD
                     YVWITPFFVSPQNDNGYDVADYYNIDPIYGTMDDLENLIKEAKKRNIGIMLDMVFNHT
                     SIENEWFKKALKGDEYYKDFYIFKNPKEDKSAPTNWESKFGGNAWEFVEEFNQYYLHL
                     FDKTQADLNWENPNVRKELKNILKFWIDKGIKGFRFDVVNLISKPEKYEDDLQDDGRR
                     FYTDGKKVHQYLKEMCEESGILENDMLTVGEMSSTTIENCVKYSNPDEKELSMCFNFH
                     HLKVDYKNQNKWMLQDCDFIKLKKILNEWQIGMQKGNGWNALFWCNHDQPRIISRFGD
                     DKKYYKESAKMLATMIYLLRGTPYIYQGEEIGMTNACFTNIKQYVDVESLNYFDILKS
                     KGMKDGEIYNILQERSRDNSRTPMQWNNSKNAGFSESKSWIEVINNYRNINVENSLKD
                     KDSIFYYYKKLIALRKKYDVVAYGDFRPLLEEHDSIFAYKRCYEKESLVVLNNFYDKE
                     IDVVLENEELEKYKCIISNYGDRKIVSTITLRPYECIALFKNEK"
     gene            57013..57717
                     /locus_tag="PC523_000276"
     CDS             57013..57717
                     /locus_tag="PC523_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2473848.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVLANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADEELSGKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCAYKIAMSEREVQAVISNKECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            57917..58228
                     /locus_tag="PC523_000277"
     CDS             57917..58228
                     /locus_tag="PC523_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2473849.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            58250..59563
                     /locus_tag="PC523_000278"
     CDS             58250..59563
                     /locus_tag="PC523_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2473850.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQAGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            59640..60950
                     /locus_tag="PC523_000279"
     CDS             59640..60950
                     /locus_tag="PC523_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF2473851.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEDG
                     KEKLEIVGNLAKRMVKKAGIDMQINLTLDRREALKDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVEGDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNNGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPKFNR"
     gene            60976..61707
                     /gene="chbG"
                     /locus_tag="PC523_000280"
     CDS             60976..61707
                     /gene="chbG"
                     /locus_tag="PC523_000280"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDF2473852.1"
                     /translation="MTKIIINADDFGYCEAVNYGIVSVHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKSLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDYDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLISLKPVIEKIVNKYNLPIRGGFEYNLEYSK
                     IVPLIDSFYKENVCEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLDKNDLVISSYRDI"
     gene            61720..62037
                     /locus_tag="PC523_000281"
     CDS             61720..62037
                     /locus_tag="PC523_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2473853.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(62159..62623)
                     /locus_tag="PC523_000282"
     CDS             complement(62159..62623)
                     /locus_tag="PC523_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473854.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     KDELLYFMEKGTAKISAKAIVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIIEKIEDVVVGTSIPYNYEKSKF"
     gene            62892..63830
                     /locus_tag="PC523_000283"
     CDS             62892..63830
                     /locus_tag="PC523_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2473855.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNAEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPIATANVDSYETDKLPSYIREELKDVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSRL
                     FDKEEETKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMISMEGLTDIDADHIVVIATESDKKDLENSAIWAQIRAV
                     KDGNVTILDSAPFFSQSYNPMGKELLLESVKNELTK"
     gene            63959..64972
                     /locus_tag="PC523_000284"
     CDS             63959..64972
                     /locus_tag="PC523_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2473856.1"
                     /translation="MTKVSKKNITYLLILVSIVVLVFGILLSITIGAKDMNLSTVIDS
                     LIKMEDGINIRIVRDVRLPRAIAATLVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFTISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVNWEGIKLLFIAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNIVRFVCIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            64978..66000
                     /locus_tag="PC523_000285"
     CDS             64978..66000
                     /locus_tag="PC523_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2473857.1"
                     /translation="MKNIIKINSKTLLVILILVVLIFITLLVSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALVGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSVWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            66015..66797
                     /locus_tag="PC523_000286"
     CDS             66015..66797
                     /locus_tag="PC523_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2473858.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPKSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMDDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDIV"
     gene            66809..68149
                     /locus_tag="PC523_000287"
     CDS             66809..68149
                     /locus_tag="PC523_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2473859.1"
                     /translation="MENQEALRHEKIWILLFKYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLIFLEDMLFILGASKDSISYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVTHYFMR
                     GKSNLKLKICNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKKYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDPDLMNIAVRGININLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPIADFIAMIIVLLFLKREFKTSQV"
     gene            complement(68240..70522)
                     /locus_tag="PC523_000288"
     CDS             complement(68240..70522)
                     /locus_tag="PC523_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2473860.1"
                     /translation="MNFENTKNKKIIATLLLTLLIIVSLTCVTFIMNLNKVTHNETER
                     YLIEISQSISTTIDVKCKSIFKTLYSVGDTYFEVNDSDKEVNKYLSKKLKLFEFDWLG
                     IINSNGNFISALKDTGNVKGQDFYIKALQGKETITPVKSPFGTYGILYSVPLYKDDKI
                     VGAVAAWNTIDTMRNLLSVESFNGEGFSQIIDQNGNFIVSSANKNAPKNVVNFFDMIE
                     HKGKQVDKNKLDEMKTNLKKNKTGSMYYTLDDGISKAMHYIPLQEENLYLLSIVPTKT
                     ASAQFDLLLKKAILINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDMEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLKPEE
                     LLCRVSNDQFNVLIKTIPQKEILSYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQEPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMIRNKYDIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHIIESIVNLTKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKTLEETSMDSFVK"
     gene            complement(70852..72015)
                     /locus_tag="PC523_000289"
     CDS             complement(70852..72015)
                     /locus_tag="PC523_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2473861.1"
                     /translation="MKLMGYLRENGQFGIRNHVLVIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKDLSSEIATTGKK
                     VDYVVIQECGGTLKTVSKGAEIVGKMAREVSKEVRVEFDMSEIILALECGGSDPTSGI
                     ASNPSIGVASNLLIDEGGSSILSETTEVIGAEHLLATRFEDGKMKDKFLKFVSDVEKR
                     AITMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADTVPTDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEVFNEIVEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            complement(72028..72303)
                     /locus_tag="PC523_000290"
     CDS             complement(72028..72303)
                     /locus_tag="PC523_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2473862.1"
                     /translation="MNIIIHANEKDNLVTCVRPLVKGEKISIDGKSYIVNDNIPVFHK
                     MATENVKKGDVVYKYGEVIGIATVDIKAGDYVHVHNIESTRGRGDKK"
     gene            complement(72324..73298)
                     /locus_tag="PC523_000291"
     CDS             complement(72324..73298)
                     /locus_tag="PC523_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDF2473863.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVYPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGNPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVILGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKAIFKTNKNIEVN"
     gene            complement(73568..75481)
                     /locus_tag="PC523_000292"
     CDS             complement(73568..75481)
                     /locus_tag="PC523_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2473864.1"
                     /translation="MKSGGIIMKEILVIAPTKGTYEKSIHIVRKNKYTNIDVMFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSDEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVIDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLNKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGSNKVIPIDVKIICASNKDLKNLMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSNKYNKTPLIITPNIKKALLQREYEGNIRELEGIMERAVIVESFDSIL
                     LEKDSNEYTNNIVDKNNTDFEYNNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIENDV"
     gene            complement(75657..76871)
                     /locus_tag="PC523_000293"
     CDS             complement(75657..76871)
                     /locus_tag="PC523_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDF2473865.1"
                     /translation="MNQEYVKGKPIFTNINKIPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CASALEELDTFIELYGNKCDYARRDTLLYTANKLEIKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIEDETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNKNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            77115..78497
                     /locus_tag="PC523_000294"
     CDS             77115..78497
                     /locus_tag="PC523_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDF2473866.1"
                     /translation="MLSKDIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNRDKVNIMKVAYKSAKEFSSDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPAQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDKPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDVLMNIDMVGNNLKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            78509..79858
                     /locus_tag="PC523_000295"
     CDS             78509..79858
                     /locus_tag="PC523_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDF2473867.1"
                     /translation="MELKSFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     QYNAVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMFIGFVVPIIEDNEQKYDGIGYNIASSIEEIKPCEIAKLGVDNALSKV
                     GGKSIPSGKYKTILFNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIMQDIGEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEVIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            80008..80541
                     /locus_tag="PC523_000296"
     CDS             80008..80541
                     /locus_tag="PC523_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2473868.1"
                     /translation="MSDAHYNMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDEKHRELNEMWVNISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGDSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            80625..81434
                     /locus_tag="PC523_000297"
     CDS             80625..81434
                     /locus_tag="PC523_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2473869.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LKYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFDDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFDYSGHAIFYDELKLFNQEFLQFLEE"
     gene            81845..83890
                     /locus_tag="PC523_000298"
     CDS             81845..83890
                     /locus_tag="PC523_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2473870.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCSIDNLIYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIIHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KIIDINTVIEIFTKLNISIDKLHIIKEKYDEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASIDKLGKFIYCNKVFSNLIGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKDISAIQVLEDKIQNKFQAKGFVSKYTFESVVG
                     ESKIIKEKINIARKIAITDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYEEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDISLISKYFLDEINSNKCFTEESMKALKLYEWPGNVRELKNLVYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSVETWNNKNILLGRNKL
                     QEILKEKGIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYIKFKGVI"
     gene            84151..85350
                     /locus_tag="PC523_000299"
     CDS             84151..85350
                     /locus_tag="PC523_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HDF2473871.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     SRVKSIVDEKIFISLHEHPVLFPEYLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     ATKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGRNILR
                     VLKQVWK"
     gene            85402..86097
                     /locus_tag="PC523_000300"
     CDS             85402..86097
                     /locus_tag="PC523_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HDF2473872.1"
                     /translation="MDYLKLANHPLLWIASTIAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCILEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            86110..86787
                     /locus_tag="PC523_000301"
     CDS             86110..86787
                     /locus_tag="PC523_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473873.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNIIILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            86952..88553
                     /locus_tag="PC523_000302"
     CDS             86952..88553
                     /locus_tag="PC523_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2473874.1"
                     /translation="MMYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDYKINGNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKYWNNFTEYCDLISKSGISINMGFLLGHGTIRWSV
                     MGGSKDRHPTEEEKNEITNIINDGMKSGAFGISTGLAYIPSKYADIDELVDIAKQIKE
                     YDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVD
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDALKNEEGRALILKEAFIL
                     GDRGSIILLNTKDIDMEKKSIREIAIQKDIDEDKLLLDLLLDGDEELIFCLGGMYRSD
                     FPDKLHDNKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFSIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIEIENYSYPRSEDIDFSKPQSLAKGVEY
                     VIVNGKIALEDGNITENKCGEVLRR"
BASE COUNT        33937 a         9873 c        15322 g        29541 t
ORIGIN      
        1 tttaatattt tatttaaaat attaaaaaat atagataaaa attgttttat taagaaaaaa
       61 ttaatatatt gacaaaattt aagtgctcat ttaaactatt aattgataat aatattcaat
      121 attaaataat aggaggagtt atgaataaat atcttctaat aaatccagta gcaggaagaa
      181 tgtatggaga aaagtttcac cttgtcaaag aaaatttgat gaaaaaaggg tacataatag
      241 cagaatgtga accacaatta gaatatgtaa aaaagcagta caaagagtat acaaaaaaga
      301 ctgagaatac aatgttggat tgtagatgtc cagaaagtat aaatctttta aaaagaaata
      361 atttgataaa tagttttgaa gtacctatga tagaaccaat attagtaagg acttgtaggg
      421 ttttatatga taaatatata aaaagtaaag atgatatgct gatagtaacc tgtccatgta
      481 cacaattaag agactttgca agtgaaaaat ttaaggataa gagtaatgct atattttata
      541 catggaaaga gtttgctgag caggaaggtg taaaatcgct tggaaaaata gatgaatcac
      601 caattccatt aggatatttc aaagatactt ttgaaaaagt gcttgaagta tcaagtgaag
      661 atgaaatctt aagtgaaata caaaaaataa gaaatggctc tgaagataag tatgacatag
      721 tagaaatgtt ttattgtaaa gatggttgta ataatggaga tggtttgtaa atggtcaata
      781 aatttaataa taaattttta aggctagtaa agagtgttct gatatttttt ataattcttt
      841 tattatggaa aattacaaat tatcttggta tttggagtga ttacatactg ccatcacctg
      901 aaaaggtata tagtacattt ttaaatatga taagtgatgg ttcgatattt attaatgtat
      961 atgcaagtat gaaaagagtt ttgataggtt ttgcaataag tactgctata ggtattcctt
     1021 taggaatatt ctttggaatt tatagtggag tatacgaata ttttaaatct ctaataaatt
     1081 ttttaaggaa tacacctccc cttgctttaa taccaatgct tatcttatgg tttggtatag
     1141 gagaggaatc aaaaattata attatagttt tagcatcctt tttcccaatc tttacaagca
     1201 cattgaaggg tattaaaaat tgtgactcaa aactgattga agtgggtagg gtatttgagt
     1261 tttcaaagct tcagataata tttaaaataa taataccaaa tgccattctt gatattgcag
     1321 ttgggctaaa gttagctctt ggatatagtt ttagagcaat tattggggca gaacttgtag
     1381 cagcctcatc tggtcttgga tacttgatat ctgatggaaa agaaatgtct agaacagatg
     1441 ttgttattgt aggtataatc gtaattggac ttttaggaat aattacagat tacatatttt
     1501 cgattattgt gaagaaggta agtaagggaa agatggtgga tgcatatgaa taatttagta
     1561 tgtgattctg ggtatataat tagaaattta tctaaaagtt atgaagttga caatgaagaa
     1621 catgtagttc ttagtgatat ctctttaaac atagatagaa accatataac agtaatttta
     1681 ggtgaaagtg gttgtggaaa gactaccttg cttagagcaa ttgcatcact tgaaaatata
     1741 aatagtggtg aaattaggtt tattaatgat aataaagagt atagacctaa tgttggtttt
     1801 gtctttcagg agagtcgatt gatgccttgg ctaaacgtaa gtgaaaatat tacttttcat
     1861 aatcaaagaa aaaacactat tttaaacaag atgcttagaa aatttaaaca caataaaaga
     1921 gttattggta aaattataaa tgataatttt agaagtttag aaacaaatga gaatttgaac
     1981 aaatcaaata aatataatag gaaaagtaat attgatgtag aaaaatatct taagatgatg
     2041 aacttgcaga aatttaaaaa ctcttatcca aatgaattgt caggaggtat ggcacagaga
     2101 gtaagtatag ctagagctct ttctttcaat ccagatatgt tgtttatgga cgagcctttt
     2161 tcggcattag attattttac aaggattgat atgcaaaatg aagttataag aatacataag
     2221 tcaactaata aaggcgtaat atttgtaact catgatattg atgaagcatt aaagataggc
     2281 aagaaaatag ttgtttttac agatgaaaga aaaataaaag aatttaatat agaagatgat
     2341 tataatagag atttaacttc agagtactat attaaattaa aaaaagatat tttaatgaca
     2401 atgaaatcaa ataaaggcat ttaaagaaat taataatatt ttaaggagta gagtaaatga
     2461 aattaaaaaa gctattgata ggtataacaa tattaacgct tgcaacagca tctttagctg
     2521 gatgtgctaa aaaaaataat ggagaaaaac tttcagaaat aaatttaact tatgtaaaat
     2581 caccattaaa tgttccttct ataattcaaa aacaggatga tttatttgga aaggaattta
     2641 aaaaggataa tataaaagtt aacttccacg aaattactac aggtccagag caaacacaag
     2701 ctttagctgc tggagaaata gactttttac atgcactagg tggtacgtca gctttaatag
     2761 ctgcatctaa tggagtagag ttaaagatat taaacactta tagcaggtca ccaaaaggat
     2821 ttatgatttt gactaataat aattcaataa aatcagcagc agatttagta ggtaagaagg
     2881 tggcagggcc aaaagggact atattacatc aggtattgat ttcagcactt gataaagaag
     2941 gattaagtat ggatgatgtg gaatttgtta atatgggtat tccagaggca tcagcagctc
     3001 tttctgatgg gagtatagat gctgcattga ttgctggtcc agcagctctt aaagctatga
     3061 agtcagggag taaacttgta gcaaatggtg aaggtttagt agatggcata atagtaactg
     3121 ctgttagcac agactttgca gagaaacatc cagaaatagt tgaaagattt atgaaggttg
     3181 aaaaggaaac tttagaatat gtaaataata attttgatga agctatggaa aaagtagcta
     3241 aagaagttga tttaagtctt gaagaaacta aagaattata tgtttggtat gattttagtt
     3301 tagatataac agataaagac atatcttcac ttgaagatac acaagacttc ttaataaaaa
     3361 ataagttaca agagaaaaaa gtgaacataa aagaattaat atataatgct aagtagaaac
     3421 tatgctaaaa taatactaag taagaaatat aataaaagtt taagttacct tattaaggta
     3481 actttttcta ttttgaatta gtaatttaaa tttattttag aatgattaat tgataatagt
     3541 gattaattaa tcataaaaag ataccttttt atagagtttt gttaagatgt ctaataatat
     3601 agttgtatct atgaagtcag taattgtgaa gttagtaata tgattaaatt tatatattgt
     3661 aaaaatatca taatatatta aaattgagct gttatatgat gagtaaaaag gtatattagg
     3721 aggaattata tgagttataa agtatatatt gtggaagatg atatatcaat aagtacatta
     3781 cttaaagatt atattgagaa atatggattt gaagtgattg ttgaagaaaa ctttgatgat
     3841 ataatgatta catttgaaag ctttaagcca cacttagttc tgttagatgt aaacttacct
     3901 aaatttgatg gtttctactg gtgtaggtgt attaggcaac agtcgaattt gcctataatt
     3961 tttatatcag ctagagatgg taatgtagac caaattagag ctttagaaag tggagcagat
     4021 gattatataa cgaagccttt ttactatgat gtagtaatgg caaagataaa gagtaatata
     4081 agaagggtat atggtgaata ttcaactaaa atagatgaaa gaatagtgga gttagatgga
     4141 ttaaagtttt atccagaaag gttggaacta gaattacata gtaagaatct aattataacc
     4201 aaaaaagaag gtatattgat tgaatgtctt atcaaaagat acccaaaggt agtaagtaga
     4261 gattatcttt tagaaaagat atgggatgat attgagtttg tagaagaaaa tactttaaat
     4321 gtaaatgtta gtaggataag gaaaagattt gaggaattag gcatagataa tgctgttcag
     4381 actatacgag gattaggata taaattaaat aaaaattggt aaggagaaag tatgaagtta
     4441 tttttaaaag attataaagg ttatattttt atatattata taggcatagt tctgacaatt
     4501 atttactgta atcttatgaa gtttattagt tttgatgaga gtttatatat tcttttattt
     4561 agttctttta tattgttatg ttttttaata tataagtact ataaaaatag agaagtatat
     4621 aatctgtttg aaaaaggaat acattctttg gaggagtcag ttttgtattt aggaagctct
     4681 ttcttgggga gaaatatatc agttatttta aaacaacaac ataaattgta tcaatcaatg
     4741 atacaagagc ataaaaagat acatggtgag catttaactt tcattaatca atggattcat
     4801 cagatgaaaa cgccaatatc agttataagc cttcagcttc aagattatga gggagaagaa
     4861 gtaactgaaa atataagaga agaaatagat agattaaata aagggttaga tatggccatg
     4921 cattttgcaa gggcagataa ttttcagaaa gactttatag ttgaaaaagc caaattaaat
     4981 gaaattgtat ctagtattgt aaatgaagaa aaaaggttat ttataaaaag tggaatgatt
     5041 cctaagattc aaatagacaa gtttacttat gtttatacag atgtaaaatg gatgaaattt
     5101 ataataggtc aattagttac aaatggggtg aaatattcta aagattgtag caactacata
     5161 actataaaat ctgaagaaaa taataagtat gtaattttaa gtataataga tgaaggaatt
     5221 ggaattgttt ctaaagattt aaaaagggta tttgacccat tttttacagg agaaaatggt
     5281 cgtaagtttg gagaatctac aggtatgggt ctttatctag taaaaaaggt atgtgatgat
     5341 ttaggtcata aagtaagtat aaaatcagaa gtaggaaaag gtacagaagt ttcaatctcc
     5401 tttaataaag atgtaaatag tttgataaag atgtaaatta tctgttacaa tagggttttt
     5461 agttaattgt acttttttat taaatgaaat attcatagtt aaataatcca aatttaaaag
     5521 aatttatttg caaaccttac atagatgtaa ggtttgtgta atgtttgcag atagttaaat
     5581 tttctaatct aaattacaat taatataaga aataacataa gggaaataac ataaaaaaag
     5641 aaattttgta aaaaaggaag gatatttatt gatgagttta gatgttattt ttatattgaa
     5701 atcagtcata atagctatag tagaggggct tacagaattt attccagttt catctacagg
     5761 tcatatgatt ttggttggta atttaataga ttttaagggg caatttgctg aaatgtttga
     5821 ggttgtaata caacttggag ccatattagc agtagtagta ctctactgga aaaagataaa
     5881 agatagtgtt agagaatttt taaaatttat atttactggc ggaaaagaag gaaagattgg
     5941 ttttagattt ggaatgaatg taattatagg ttgtatacca tttgcgatta taggagttct
     6001 attttacgat aatataaaat cattgtttaa tttacaatct gtaattatag gatttatagt
     6061 gggtgggatt ttattattag tagtagaaac tttgtttaga aaaaagaatc attccacaga
     6121 taatatagat aaaattactc caatacaagc tttaaaggtt gggacattac aagttttgtc
     6181 tgcatggcct ggtatgtcaa gaagtgcatc tactattatg ggtggatgga tagcagggct
     6241 taattctcct acagcagcag aattttcttt ctttttagct gtaccagcta tggtggcatc
     6301 ttcaggaaaa gatttatttg aatttgatta ttcagtaatg acaccaacat tgtggattgc
     6361 tttggtagtt ggatttatag tagcttttat tgtatcaata atagttatgg aaaaattcgt
     6421 gaatttttta aagaaaaaac caatgagagt gtttgctata tacagaataa tcatgggtgc
     6481 agtacttgca gtattagctt tgactaacat aattagtata tagaaaaaat agatttatta
     6541 tctatagaaa ttaaactaag agaaattata attaacaatt atatatatgt tatttcaaaa
     6601 attaaattag gaaggtatgt tatttcaaaa attaaattag gaaggatagt taaataatga
     6661 aaattttaac tgtaaataat ttaagtaaag tatatggtaa aaaaataatt ttcaatgcat
     6721 taaatgacat aaattttagt atagaagatg gagaatttgt aggaattatg gggccatctg
     6781 gcagtggaaa aactactctt ttaaatatga tttctactat tgacaagcca actacaggaa
     6841 ctatggagct aaaggacaag aatcttttat tgttaaaagg tgaagaactt gcattattta
     6901 gaagaagaga attaggtttt gtatttcagg attttaattt attggataca ttaactatag
     6961 gagagaatat agtattgcca ttgacattag ataaggtcag tgtaaaagag caagatagga
     7021 gattgaatga agtatctgaa attttaggaa taaaagattt attgggaaaa agaacttttg
     7081 aagtttcagg tggtcaagcc cagagaactg ccattgctag agcattaatt aatagaccat
     7141 ctatactttt agcagatgaa ccaacaggta atttagattc aaagtcgtca aagactgtaa
     7201 tggagctatt tcaaaagata aataaagaaa ataaagttac tactatgatg gtaactcatg
     7261 acccattagc agcaagttat tgtagtagaa ttttattcat aaaagatgga gctatataca
     7321 atgaaatata taaggggagt agtagagagc agttttatca agatataatg gatgtactta
     7381 cattattggg aggagataat taatgaacat aaaacagttt gctattaata atatttcaag
     7441 aaatatcaaa gcgtatttag gatatttagc aagtattact atatcatctt ctctactatt
     7501 ttcttttgtt atgtttacga gtcatccaga tttagattta tctcaatttc ctgattattt
     7561 aaaagaaggt ctcaagatga gtaagataat tgcatattta tttttattct tctttgtatt
     7621 ttattcagtt agtgtatttc ttaaaagtag gtataaagaa tttggtatac tttatattac
     7681 aggtatatca aaccgacaag taatgaaatt aatctttata gaaaatatga taataaatat
     7741 agtatcttct gtaattggaa taataatagg cttaatcttt gcaaagatat ttttagtggt
     7801 gatgtctacc tttttagaat tatcaccatt aaaattctat ataccattta atgcgatatt
     7861 gtctacttta atctatttta tgattttagc tgtattaaca tcactattta cttcttttat
     7921 agttaaagaa aatcaggtgt tgaagttact taaaggtaca aaaactccaa aaccagagcc
     7981 taaaacatca ctaatgcttg ctatattaag tatttgtttg tttataatag cgtattataa
     8041 tgctgttaca tcaactgacc aatatacaac atacctgcgc ttagttccag ttacatctct
     8101 tactatagca gctacctact tatttttttc tcaatttagt gtgtttttta taaagaaact
     8161 aaaattaaac aagagatttt atagaaaaaa tacaaatatg ctttggattt caaatttaat
     8221 ttataaggta aaggataatg ctagaatatt tttcttgata actataacct ctgcagtagc
     8281 atttactgct ataggtacag tttattcatt ttggaaagat gtaaagcgtc aaattaattt
     8341 aatatatcct aatacaatat attattcaac aatgaccttg cataatgacc ctaaaaagcc
     8401 tgatagcaaa gaaaaagaca aggaaagaat gagatttata gaaaataatc taaaaaaaga
     8461 ggatatagac tatacaagaa ttaatggcga gtttaaaact gtatttccca agaaaagtga
     8521 ttttaatgtc agaattatga aagaatctaa gtacttagaa ataacagaag ttataggggt
     8581 taagcctata agttttgaag ataatgaatc tatatcatta ttatcatcaa gtttaccagg
     8641 aaataagaaa ataaaagaaa atgttactgt aggaaataga agcttaaagg tggcaaaaca
     8701 agtagaagaa tgtgttatgc cagcatatta taaaaatatg tatgtggtaa aggacaattt
     8761 ttatgatagt atagaatcaa aatttatagt ggatagattt actgcatttg aagtagagga
     8821 ttcaagtaaa actatagaga tatgcagaga atttgaaaat aaatttgata atgagtcggg
     8881 aatgcaacca tatttgtttt tctctaaaga attaatgata gaaactggta aattaatgta
     8941 ttctacattt atatttttag caatatttat aggtttaatt ttcttcgtta ctacaagtag
     9001 cttcttatac aacaagtttt atatggattg ccaagttgac aagaaaaagt atgaacagtt
     9061 aaataaatta gggatgacat ataatgagat taaaaaagct tcaactattg agattggcat
     9121 agtatttttg ttgccttatg tagtggctgt tatacattcg atatttgcac ttactgctct
     9181 gaaaaattct tttgatttag aagtagcatc atcagctgtt ttggtaatgg gaagcttgtt
     9241 cttggttcaa atagtttatt ttttaattgt aagaaataat tatttgaaag agataaaatt
     9301 aaatttagtt aattattagt tactattatt aaatgataac aaaaagggtt taaaattaat
     9361 taatattact ggaattatag atgtttattt tttcagtaat attttttatt gtattaacta
     9421 ttaaaatttt gaacaaatat aaagtataat atgataaatg tcaaaattat aaaaaagttc
     9481 tacttatata aaatataaag aggttatatg tacatatgtg tagaaataaa taataaaaat
     9541 atcgattaat gttattaggg ggtgttactc attagtaaat tagctaatac acttagaatg
     9601 atgatattaa ttcaaagtag aggcaaaatg aaatgtaaag agttggcaga agagttagaa
     9661 gtaagtgaaa ggcaaataaa aagctataaa atgtacttag agcaagctgg tatatttata
     9721 aattcaacgc ctgggattta tggtggttat gaaatagata aatgtaacag catctcatta
     9781 attaaattat tagatagtga agtttcaatt ctagatatga taaattcaca gttagagtac
     9841 aataatgata tttacaaaac tgaatttaat agtattgtag aaaaaataaa agcagtatta
     9901 aatacaagag taaaaagcga tacatatatg gattatttca caatacaacc acaacgtaat
     9961 tgtgattata agtttgagaa aaataagtgt aatgaaataa ttagagcata tacaacaaag
    10021 cgtaaacttt ggatagagta ttattcattg aactcaggca atagtgagag aattgttcat
    10081 ccatatggtt tgtttaatta taagtcagat acttatatgg tggctttttg tgaaaaaaga
    10141 tttaagttta tagactttaa attatgtaga attaaaaatt ataaggtttt agaagaaaag
    10201 tataatgtag acaaaagttt tagttgggat gaatatagta aaaatagtat aggaatttat
    10261 aagggagaag aaattaatgt tgttataaaa attagtcatc cattttcaac aattataaag
    10321 gagaaagttt gggttaataa tcaacaaatc atagaatatg atgataaatc tataatgttt
    10381 aaagctaaaa tgagaggtta tgaggagata aaaagttgga ttttaagtat gggagcttat
    10441 gttgaggttg tagatccaga tagccttaga aacgatatat tatcagagat tgaaaaaatg
    10501 aaaaaaattt actgattttt tcaaataatt tatacttgga ccaatatact tcacggtcta
    10561 gtgttaatat attaattaag agaggtgata ttatgaaaat gaaaattgaa tttagtacaa
    10621 gttgtatacc aatatcttat aattcgctct ttatgagtat tataaaagaa gcaataaaaa
    10681 aatctaatga agattactat aaaaacatgt actattataa agaaaagaat aataaaaaaa
    10741 ctaaaaattt tacattttcg gtttatgtaa aaaagtatag tattgaaggc gacaatttta
    10801 ttatagaaga taaagtaata ttaaatataa gtactccaga tttagaattg ggttttcata
    10861 tatataatgg gttaatgact tctaaaaaat gcttatataa agactatgaa cttacaagaa
    10921 ttagaatcga tttatcaaga gaaaagaagg taacagaaga aagggtacta tttaatgcat
    10981 tgtctccaat ctgtgtaaag tctaaagaag gaaaattctt ggagataact gatgatagat
    11041 acatagaaga actaaactat ataactaatg aagtggttaa aaattatagg ggtagtggat
    11101 taaaaagaaa gttagaattt gaaaatatag atctcaaaaa ggtagtagtt aaagagagtt
    11161 taagagaatt taaaaaaata acaggtaaag agtatcaata tattaatggt tataaaggta
    11221 aatttatatt gaaaggtgat attgatgatt taaatattat ctacaatcta ggacttggct
    11281 ttaggcgtgc tcagggattt ggagatgtag atattttaga atggaggtga gataaatgaa
    11341 gttaagagtt tatagaggag attggttttt taatatgggt atagttggat ttttaaatat
    11401 aataaagaaa tcaaataaac aagcagaaat atttattatg gaagattata tcgaatttga
    11461 tagttcattt cttgaaaatt ttcatgaata ttattttaat tactttatgg atgaatatga
    11521 tgtatcaaaa aggattaaaa aaagtattga ttacagtata aattttatta aatccaaacc
    11581 tgataaagtc aaagatggaa ttaaaaaaat taaagacacc gtaaaacaac aaaatgataa
    11641 ggttaaaaaa tttgatgaag aaaaatttaa cttgataaaa gagaagttag atagcatttc
    11701 taagataaaa agttatgatg aatttgatag tttagaaatt ttagtggatg agactataga
    11761 tatatttaaa ataaaatcaa taaatgacag gcttacagca aatctttata aatatgtagt
    11821 tcaagataat tattttggac aagttagttt ttttaatgtt gctaaggcta agttagattt
    11881 ggatggatta aaacaggtaa tgtttaatga ttatttaaga caaattatat attttggaga
    11941 gttagaagat ttattagaag agaatgacta tgacaagttg aaaaactatt tgaatgatag
    12001 attaaatagt atagctaagg atataagtga aaaaaaagtt agtaagtcta gtgtaaatac
    12061 tatagaaaaa attatgaagg aaataaataa aaactttata aagaaaaata aaagcataga
    12121 aaatataaaa gagtatatgg atagtttaga aatatgtgaa atgtgtggtt tatataaagg
    12181 aatattagat gaatactcag aaagtaattt tgctccactt ggagtaagta ccaataatgc
    12241 taggaatatg ttttggaagc aggagtatgt atcatcaata tgtgatgttt gtaagttaat
    12301 attgttctgt actccagcag gggctactta tacaagaaag gattatttga ctaatgaaga
    12361 aaatgagttt tatttatttg ttaacatgga tacttctata aatgagcttt ttgatagaaa
    12421 caatagcttg aaagctcaaa aaagtgagtc ttcaaattct aaagatgaaa atccttttaa
    12481 tcaacttata aaaagtatag tagaagaaaa taaattaaaa agtgaatggc aacttcgtaa
    12541 tatacttttt gttgaattta aagcaagtat agattctaaa aaatgtaaaa taaattattt
    12601 taatattcca acttatttag caaagttttt tgtaaaagaa aataaaaaaa tacaaagtat
    12661 atatgattat aaacttaaat caaatgtatt agatttaatt ttaaaaagta aagacttaaa
    12721 acatttaatt aataacactt taaggaatat agttaagagt aatttagaaa gtaataataa
    12781 atcaaacata tcagggttat cttgtttcaa tgttgttcaa ttaagaacaa tattaaataa
    12841 ctataagaga ggagtgtata aaatggacta taaaaagttg agaaagattt atgagtcagg
    12901 ttgtgaaata catgattatt atattgataa gaatgctaaa aataaaatag aaggaataac
    12961 atataaaatt ttaaatttaa ttaaggttgg taataaaaat gattttatgc aaacaatttt
    13021 aaggattttt tcaccaagtg aaaaaacacc accagaagaa attttacaga tatttgttga
    13081 agaagattta gattttgaat ctgtagggta tgcatttgta acaggattaa tttctggaaa
    13141 atatgaagga aaagataaac tatcaaacga gaaggaggaa aaataagatg aaaagaggat
    13201 taacatttac agtaatagca caagctcaaa gtttaaacta tggtgaaggg ataggtaata
    13261 tatcagaatt aaaaaaatta agtagagctg atggaaatat atatacattt gcatctcgcc
    13321 agtgtttaag atatgacata gtacgattag cagcagaatt gtttaattgg aatttacagg
    13381 ttgtcgataa agaaaaaggt actattcaat ttaaagataa tataagtata agagaatcag
    13441 aagaaatgga tttatttgga tatatgaaaa caaataaaaa agatgagaaa gataaaaaag
    13501 gaggttcttt gactagagaa gctgctgtaa gattaagtaa tgcgatttcc cttgagccat
    13561 atagaagtga catggacttt ttaaataata aaggattagc agatagaata ggagaacatc
    13621 ctaatcttgc taacatagag caacatttaa gttattatac atatacagtg actatagatt
    13681 tatctaaaat agggaaggat ggagagatag aacttgataa taaagagaaa tgtagaagag
    13741 tagtagaatt tttagaaata ataaaagtat tgaatagaaa tattagaggt agacaagaaa
    13801 atttatctcc tctatttgta gtaggtggag tatatgaaat agcaaatcca tttttcttgg
    13861 gtagaattaa attaaaagga gataaaaatg gatttaaaat aaataaacaa gctatagaag
    13921 aagtagtcca aggtactttt ttaggaaaat atttaaaaga atttacctat gttggaatgg
    13981 tagatggggt atttataaat aaagaagaat ttaagggatt gtttgaagat aattttttaa
    14041 gtgtagataa attttttgga caactagtta aagaagtaaa ggaatattat ggggtgaatt
    14101 aatatgaagg ttttgaaatt agatttattt caagaaactg catgctataa aaaaccattt
    14161 gctatgaaga ttacagagac atatccactg cctccatatt ctacaataaa tggattaatt
    14221 cacaaaatat taaatgctac agaatatata ccatttaata taagtgtaca gggaacatat
    14281 gaaagtatat ttaataatta tcagacgaca tatttttata aaaaagatag tattacatct
    14341 atgccaatga atagtcatat gctattgaat gtaaatttga taattcatat aggtggggat
    14401 tttgatttat tgaaagaaat atatgatagt ttattaaatt ataacgagca tttatcatta
    14461 ggtagaaaag aagatttagt aagaattact gatattagat ttgtagaagt taataaattt
    14521 gagatagatg aggacataca attagatgat tatgaaaatt ataagttatc agaatgtgat
    14581 ataagaatgc ctatatatat accaaaaaca agtttacaag ataaagatat agatggtata
    14641 agttacaggc tcaataatta ctatgataat aatgctgtgg gagataaaaa aagagtttgg
    14701 aataaggtgg atagttatta tgttgaggat ggaaatacta tatcagttgg agatatatta
    14761 ttagataatg aaggagattt attatatttt aataatataa attaggaggg agagtatgtt
    14821 atatgctaaa tctaatccag tggaaacttt gagagaacat actgatgaac ttttaaaaca
    14881 aatgaatgtg ttaagagaaa gttatggaac aaatataaat agcttaaatt ttctagagga
    14941 ggagatattt tggcaattac ttgactttgt tatagaattt catgatattg gaaaagcatt
    15001 ttcaccattt caagagttaa taataagtag aatggatact aaaataaatg aacctaaaat
    15061 agtaactcat ctagaaaata atgtaggtca taattatctt tctcctgcat ttattgatta
    15121 tagttatata gacaagaaaa aaaataaaga acttagagca gtattaaatc aagttattgt
    15181 atatcatcat gaaagagata tctttataga taaagatttt aaaaatttaa tacaaaaaat
    15241 attagatgaa gatttaataa ataaagttta tgaactacag catgaattta aagttagata
    15301 tccaataaaa acagaaaaat tgagtaaagt atatttacaa agtgtagaaa aaagaataga
    15361 taaaaatcac aagtactata atttatatat tatgttaaaa ggaatactac ataggctaga
    15421 ccatagtgct tctgctcatg aagttgtgga atgcaataat gttatgaata taggagaaca
    15481 cacagaaaat tatttaatga aagaatttgg tagtttgaga gaaactcaat catttgccca
    15541 atctaataga aataagaata taatactgat tgcatctaca ggaatgggaa aaacagaaac
    15601 tgccttaatt tggatagata aagacaaggc attttttaca cttccattaa gagtaagtat
    15661 aaatgcatta tttgatagag caaaaaatat agttggagtt ggagaatcaa atgatacatt
    15721 cttaggattg ttacattcaa cagcaataga ttatctggaa gaaagtaatc aagaaaattc
    15781 aagtgagatt gtagatttag caaagttatt atcctgtaag ctgacatttt ctacaataga
    15841 ccaaatattt aaatttcctt ttttgtatag agggtatgaa aaagtgtact caacattagc
    15901 atattcaaaa gtagtaatag atgagataca agcatattca ccagaaatag cagctgtatt
    15961 ggtaaaaggt atagaaatga tacataaaat aggcggaaaa tttatgataa tgacagccac
    16021 tatgccaact atatatatag atgaactaaa aaaaagaggt gtagttgaca gtaacttagc
    16081 taacttgaca tgtaacacag aaaaaataag acattgtgtg tctattgtgg aaaattcaat
    16141 agatgaaaat ttagaacaaa taatacaaag tggaataaat aaaaaagttc ttgtaatagt
    16201 aaacacagtt aaaagtgcta tagaaaaata tgaattaata gaagagataa ttaagtcaaa
    16261 aggaatagat attaatgtaa atcttttaca ttctatgtat atacaagaag atagagcaaa
    16321 gctagaaaaa tatataaaag aatttgcaga tagtgatagt aatggtattt ggattactac
    16381 acaattagtg gaagcatccc ttgatattga ttttgatgaa cttcatacag aaaattcaac
    16441 acttgacagc ttatttcaaa gatttggcag atgttataga agtcgtgagt atgaagaaaa
    16501 tagtccaaat atatttatat atacagaaaa agctacagga ataggtagta tttatgataa
    16561 agatattgta gaaaaaggac ttgagctatt gaaaaactgt atgaatggaa aaaaatgttt
    16621 taaaatgaaa gaaaaacata aagtacaaat ggttaagata ctatattcta aagaaagttt
    16681 aaaagataca gattttgaaa aaaaatttac tcgtgcttta gatatattag atacgataac
    16741 accatatagt ttaggctcta acgaggcaca aaatatactt agaaatattg aaggatatac
    16801 agtaataccg gaggatatat ataggaaaat tgaaaaaact ttaataagag attatgaaga
    16861 gttgggacaa gaacttacag aggcatatag caatgatgat agacaattga taaatgaaat
    16921 aaagtctaag agaaaaaaag caagaagaga gataattaaa aaaactgtaa atttaccaat
    16981 atataaagct aaacaaaata tagtagatat acaggtgaaa ggtctggaag atttaaagat
    17041 acttttatat aaatatgata tacatgaaga tgtgcataca aaacatatat ttggcaaagg
    17101 tgtgattata ggtaaggaaa tagaccaatt tatttgattt ttatattaac aatgtggcta
    17161 aaatttacta aagaaggtga gtttgtgcca atagatttag atttattgaa agttcaaggt
    17221 gtaaagataa attattatta tgtatgtaaa agaaaactat ggctattttc taaaggaata
    17281 actatggaac aaaatagtag tagagtattg tctgggaaga tagtacatga agattcatat
    17341 aaaagaatga aaaataaaga aatgttgatt gatgatatct tgaagctaga tataataaag
    17401 gacgagtatg taaaagaagt aaaaataagt tcaaagatga aagaagcaga tgaaatgcag
    17461 ttatactatt acttatacta tttaaagaat agtatgggta tagagaaaaa aggaactata
    17521 aattatgtta aggaaaagaa acaagaagaa ttgatattga cagaagaaaa agaaaggatt
    17581 attgaaaaca cacttataaa tataaatgaa ataaacatga aattatctcc tccaaaaatt
    17641 gagaaaaaac cttattgtac aaaatgtgct tattatgaat tttgttttgt gaaggaggag
    17701 taacaatgta taaagactat tatattataa gtaatggtaa tataaaaaga aaagaaaata
    17761 caatttacta ttataattta gaaggtgata agaaaatatt accagtagag caagttgatg
    17821 atattcatat atatggtgaa gttgatttaa acacaaaact tataaattac ataagtcaat
    17881 atggagttat gcttcatttt tataattact atggatatta ttcaggtaca tattatccaa
    17941 ggaagaaaaa tgtatcagga ttcagtttag tatgtcaatc agcctgctat ttagataaaa
    18001 atgaaagatt atacttagca aaatcctttg tggaaagtgc atcataccat attttaagaa
    18061 atatgagata ttacaaagtt gatgaaagtt taattgatgc tataaaggat gaactaaatg
    18121 atttgaatga tgtcaaagaa atatctgaac ttatgggtaa agaaggtaga ataagaaaaa
    18181 catactataa atcatttaat caaatattaa aagatgactt tgaattttat aaaagagaaa
    18241 aaagacctcc caaagaccca gtaaatgctc ttatgtcttt tggaaatagc ataatgtata
    18301 ctaatgtttt aagtgagata tataagacac aacttgaccc tacaataagc tttttacatg
    18361 aaccatcaac aaagagattt tctttaagtt tagatattgc agaaattttt aagccaatta
    18421 taattgacac aataattttt agcctgatta ataataaaag aattaataag aatgattttg
    18481 aatatcaaga taaaatttgt tatttaagtg aaaaaggaaa gaagaaattt ttgcaagaat
    18541 ttgaaaataa gatgcagaca accataaaac atagaaatct aaatagaaaa gtttcatata
    18601 gaatgtttat aagattagag tgttacaaat taattaaaca ttttataaat gatgaaaggt
    18661 acaaagtttt aaaggtgtgg tggtaagtat gtttgttatt gttacttatg atattgttga
    18721 agcaaagtcg ttaaatagaa ttagaaagat acttagaaaa tatttgactt ggacgcaaaa
    18781 ttctgttttt gaaggcaata ttactgatgg aaagttacat aaatgtattt ctgaaataga
    18841 aaatattatt gataatagag aggattcaat ctatatttat gagataaaaa atcctaattc
    18901 aattaaaaag aaatgctatg gtattgataa gtattctgat gaaatgttta tataggtttg
    18961 cagtgagcga catttatgct aaaataggtg ttaacagttg gaatataagg gattgaaggt
    19021 gtatgataac tgttatcaat tgcactactg ttcgctcact gcaaattttg atgtttttat
    19081 tgaattataa ttgcttgatt gaagtatttt caatgtattc aattacacct attttggggt
    19141 tttatattaa ctaagtggta tgtaaagcaa tatattctag caggagcaga tttaggagct
    19201 ttacaagttt tatattaact aagtggtatg taaagattct ttatacagag aaacacgata
    19261 ctatttacag gttttatatt aactaagtgg tatgtaaaga tatctttgta ctcttgattt
    19321 tccagctttg atatagtttt atattaacta agtggtatgt aaagaattgc ataatattag
    19381 agagtttaat accatcaagt gttttatatt aactaagtgg tatgtaaagg atggtaaaat
    19441 gggagatact ttctttggat taacaggttt tatattaact aagtggtatg taaagttgct
    19501 tatacttaca actttttctg ctgtagcatg ttttatatta actaagtggt atgtaaagaa
    19561 aactgtttct acttttttag tcccaagtaa ttccgtttta tattaactaa gtggtatgta
    19621 aagcgcattt ttgcttttat attgcttctt ctatcttcta gttttatatt aactaagtgg
    19681 tatgtaaagt ctaaaatatc ctcacaactc ctgtaattgt attttctgtt ttatattaac
    19741 taagtggtat gtaaagtggt ttactaagaa aataacggat ttttaagaga aaagttttat
    19801 attaactaag tggtatgtaa agtttgatga aaaagaagaa tataagaggg atgtggcaag
    19861 ttttatatta actaagtggt atgtaaagta actccaaact ccgtaagtct tgccatttct
    19921 ccaagtttta tattaactaa gtggtatgta aaggagtatc aagagaccag ggaaaggcag
    19981 gtaaagcagg ttttatatta actaagtggt atgtaaagaa tggtaaaatg aaggcataat
    20041 aaatttcaat ataggtttta tattaactaa gtggtatgta aagagagaca gtttaaaact
    20101 aaatcagaag aaaagtctta tagttttata ttaactaagt ggtatgtaaa gtttaaagca
    20161 cccttaaaat ctggttgggc atgttctcgt tttatattaa ctaagtggta tgtaaagttg
    20221 gtagacaacc tggtacttgg cttaagattg ataagtttta tattaactaa gtggtatgta
    20281 aagcatgtga tagagataat atacaatatg atagagaaag ttttatatta actaagtggt
    20341 atgtaaaggc ataaaatacc ttcaaaaata gcttctactg acagttttat attaactaag
    20401 tggtatgtaa agcgaaataa tgttttgttt tcgatgggta tatatacggg ttttatatta
    20461 actaagtggt atgtaaagta gcttttgcga ctggaagata cgcagataat ttagttttat
    20521 attaactaag tggtatgtaa agattgtaag ctctttaatt tctaaattag ctccgattgt
    20581 tttatattaa ctaagtggta tgtaaagtta agtagagcaa tgtatcatgg ttatgtgaaa
    20641 agtgttttat attaactaag tggtatgtaa agctagtaaa ttttgctaaa aggcactatt
    20701 attgtagtgt tttatattaa ctaagtggta tgtaaagcag ttgacacaat gagtaaaagc
    20761 gaagcagagc aagattttat attaactaag tggtatgtaa agttatgtta taggagcagg
    20821 ttgcaagaag tataagcaag ttttatatta actaagtggt atgtaaagca tagattttgg
    20881 agatttaagg gaaggcaata tgtgtgtttt atattaacta agtggtatgt aaaggggttt
    20941 gcatctttat tagataattt tattaataga gttttatatt aactaagtgg tatgtaaagt
    21001 aaatatagaa taaagcattt tagtttacaa tgcaagtttt atattaacta agtggtatgt
    21061 aaagacctaa aaaatcaatg cagcttatct tatatgcaat ggttttatat taactaagtg
    21121 gtatgtaaag attaaggaat agtaattaca gtggactgga gttagtgttt tatattaact
    21181 aagtggtatg taaagacgtt tcgcaaggga ttttttaaag cgaatggata agttttatat
    21241 taactaaatc atatctaaat aaaatacact tacctataaa catgataaaa tcaatacaaa
    21301 aatgaggtga aacaaaattt atgataagga aattaaacaa taaagatata aataaaatca
    21361 tggaaatatg ggaagaaagc acaatcaaag cacatggctt tataagtaaa gaatactggc
    21421 aaaataacca taatgctgtt aaaaatgagt atatacctat atcagataca tttgtatatg
    21481 atattggaga tgaaataaaa ggatttataa gcataataga taaaagtttt ataggagctt
    21541 tatttataaa gcccaaatac caaaatctag gtatcggagg taaactttta gattatgcaa
    21601 ctgaaaaata taaaagtcta agtttagcag tatacaaaga taataaaaaa gcagttattt
    21661 tttataataa aaaaggtttt aatgtagtaa aagggcaatt aaatgaagac tcaggattta
    21721 aagagtacat aatggaatat agtaaataat atgagtgtat tctattaaaa agataattaa
    21781 tctatttgat atttgattag aggcattttt ataagagtaa tttttttaat agattatata
    21841 aagataaata tatttgttat atatgtaagt taatcagaat tgctatggtt acaaaagagg
    21901 aaaataaaat tgtatggtgt atttcaaatt taacaaattt accatttaaa aataataagt
    21961 ttgatataat attaaatgta ctttcacctg taaactataa aagatttgaa agagttttaa
    22021 ataattatgg aatggcaaga aaggtttgtt aaaatttaat aattattaga ttatagaaag
    22081 ttgccataaa attagttcag taagaactag tttcatggca attttaattc ttatgagagt
    22141 atttattttt acatatttta aaaacttata cagtataata atgtaatctt actatatcag
    22201 tctatagata tttaaatctt aaaatgttct ctttgagata agtttatttc agagtcacaa
    22261 tttccacaac agtaagatgt tgagacatct ttaaatttct cagtattctc atcaaagttc
    22321 atttttttct ttgcaataat tcctaccttg gttaaattag agccacaatt attacagttt
    22381 tgaattttgc ataggttaga tagtttatca agtgtaacat cttctaataa ttcatcacaa
    22441 ttacaacatc tgatagaaga aacttcatta gatatttcaa taggttcagc ttctttagaa
    22501 gaaaatttat atgttatgtc atttgatact ttataacctg tgtcaaatat attagaatta
    22561 caacttggac ataggtactt tggaagatat atatcttttt tttcaaaagt atcactacta
    22621 gtattatagt ttaattcatc taaaattggt ctttcatcta ggttattatc tttatttaag
    22681 tttagaagat aattataaat ttttttatct tcattctgtt ttgatgtatc caacaagtgt
    22741 tccattatat tagtgtattt tttatttttt gaaggcatgt ttaaatccag ataattgttt
    22801 tcaatagcaa agcacactat attgaaatag ttatatttaa ctgcaaaaaa taataacatt
    22861 tcatcaaata ctttatctat tattttgtca gaaatactta tccaaaaatc tggtgactct
    22921 aagtaaactt tcctactaag ttcatctaac tcttcatcta tattattttc attaactttc
    22981 atattatcac ttccttttaa atatcttgtg tataaactat ttgttactgt tttttatata
    23041 aaataaaatc ttatataaaa atatatcata ttataattta caagtatagt ctaaatagag
    23101 ctgtttttaa gcaaagtctt ataaatgact attaaaacat aaagtaacag tgtaaagttt
    23161 acttataaat taaatgtaaa cctaaatact tataagggtt gacaaaaaaa cgcctttatt
    23221 ttatactaaa acaaagcttt aatagaagct ttgtatatgt acatataaac taaattgaat
    23281 aagacatatg aagggaatta taaaaatgaa aaatactaaa gtaaatattc aagatttaac
    23341 taaaatgtca atatgtgtag cattattgtg tatatcatct tacatatata taccattgcc
    23401 atttactcca gcaggagtaa cagcacaaac aataatgata aatttgatag ctttgatttt
    23461 aacaccaaga caagcattta gtacagttgg tgtatacata atgataggtt taattgggat
    23521 tccagtattt ggtggaggta catctggtat tggaaagttg ttaagcccaa caggtggttt
    23581 ttattttggg tttttgtttg cagcactaat tattagttta ttaaaaggac gaaataatga
    23641 tattaagaga tatatactaa taacaatttt tgttggaatg cctattatat attttatggg
    23701 aacagtgttt atgtgctatt ttaatcaaat gacagtagaa gcagcacttt ttgcagcagt
    23761 agtaccattt ttaattggag atacaataaa gtcagttatt gcgtcttttt taggtactaa
    23821 actgaacaat gtactcagaa aaaatctata atataaggaa gggtgtaata tatggaggag
    23881 gtttttatat taggaggatt gagaagtcat ataggtctaa aaaatggaat ttttcaattt
    23941 gttcaacctg aattactagg agcaagtgtt ttaaaacatt taattaagaa gtatgaaatt
    24001 gataaaattg atgagattat atgtggaaat gctgttggga caggagggaa tatagctaga
    24061 ttaatgactt taacagcagg agtgtcaaat gaagtgcctg cttttactgt ggatatgcaa
    24121 tgtgcttctg ctatgatgag tatagacata gctttttcaa agattaaatc tgggcagtgt
    24181 gatttaatca ttgcaggtgg ttttgaaagt agttctctac aaccaatgcg tacatatcat
    24241 aagaatgata agcgatataa tataaataat ccaaattata cagttgctca attttctcct
    24301 gatgataata gccaaaatag tatgctagaa ggggcagaaa gagtagcaga gttatatcaa
    24361 attaagaaag cagaattgga tttttgggta aaggaaagtc ataaaagagc aaaagaagca
    24421 agagaagaaa aaatatttga tgatattata tttcctataa ataatagtac atgtgatgaa
    24481 ggtatacgtg ataagatgag tcaaagatta ttagatagaa tgccatctat tttaggaaaa
    24541 gaaagtatta cgaatgcagc aaattcatgt cttattaatg atggagcttc atttattata
    24601 ctatgttcta agaagtatct agaacatgca aaaaataagc ctatagcaaa aattgttaat
    24661 acatgtacaa tagggacaga tgcaaattta agcccaacat cagcgataaa agcaatggat
    24721 aaacttttag aaatagaaag ccttagttat ttagatgtat ctgctgttga atttaatgaa
    24781 gcatttgcag ttatagatgt gctgtttcaa agaaagtatc cagagttaat agatagatat
    24841 aatatatttg gtggagcttt agcgtatgga catccttatg gtgcttcagg tgcaattatt
    24901 gcattacatc ttttaaaagc attagaaaag actaagggta gatatggaat ttgctctata
    24961 gctgcagctg gtggtctagg ttcagcttta atgattgaga gggtttaaat tatgaactat
    25021 tatgaacttt taagaataaa aagcgatagg cataaaaata aaaatttttt aattatagat
    25081 ggagaaaaat atacttatga aaatatattg agttatagtg aggaaatagg aaaacaaatt
    25141 tatttaggtg aaaatattcc tattttaata tattctaaaa atataatgtt tcagttaatt
    25201 agtttttttg ctattaatta ttcaaaaaat attcctatta tttgtcatta taatctttct
    25261 aaaaaagtat tagatgatat tttactaaaa aataatattt caatcattat atctgatgaa
    25321 tctatggacg ttattgattt atatgatgat aacaataatg aagtagcaaa aaaagttaat
    25381 ttgtctattt ttaatcctaa attcaatata tatatgtatc aatataagaa acttataaat
    25441 tatttagata aaagcatttg tatgggagct ttatcttcag gttctacaag tgttccaaaa
    25501 gtattatata gaacgtatga gagttgggct ggattttttc ctatacaaaa tgctattttt
    25561 aatatctctg gggaatcaat cttgtttatt aatggaacac ttagttttac agggaatcta
    25621 aattctataa tgtcagtatt atatgaagga ggtagtattg taataagttc aggtcttaat
    25681 tgtaaatcat ggattaggac aataaaacaa tataatgtaa ctaacatata tcttatacct
    25741 ataaaacttc aacttttggt taaacactta aaagaatcag tactcaaagt tgtaagtatt
    25801 tttacagggt ctcaattatt atttgaagat actgcaagaa atctaaaaaa atatatgcca
    25861 aagtctgaaa ttattttata ctatggggca agtgaattaa attatattac atatttatgc
    25921 tatgatgaac taattgaaag acctctaagt gtaggcagac catttccagg aatagatgta
    25981 tatattagag aagaaaaaat ttttgtgaat acagaatatg cagtatataa tgcaaccaaa
    26041 ccttattctg tcaatgatat tggatatttt gataatgatg gatacttaat atttgaagga
    26101 agaagtgatg acattgtaaa tattggagga tttaaagtta gttctactaa gattgaaaat
    26161 gaagttaaaa aaattcctca aattgaagat gcagttgtat tgccgtattc agacactata
    26221 agaggaaatc aaattgtatt atttgtaata actatagaca aaattacgaa gaaagatttg
    26281 ttgattaaaa tgaaagataa tctaatgaaa aatgaaatcc caaagaaaat tatcttttta
    26341 aattcatttc catatacctc ttctgaaaag atagatagat tagcattatt aaaaatgtta
    26401 taagtataat atttagtaat tttagtgaac caatgtattg ataaaaacat attgtatata
    26461 gtaaaggagg cttacaatat gtcaaatcaa tacgatataa ctgttattgg gggagacctg
    26521 cgacaagtgt acatggtaaa aaaactaatt aataaagggt attctgtagt cacttatggg
    26581 atatcaaatg aaattataaa tggaatcgtt ggaaaatcaa cttcactagc tgaagcacta
    26641 tccaaaagtt ccattatttt atgtccgatt ccatttacaa agaatcaagt taacattgaa
    26701 aatactgata attgtcttga tgctactatt gataacttgc taattaattt atctcctaat
    26761 caaatcttat ttggaggtaa cataccaact aaagtttgta aattttgcga gcaacactct
    26821 attcagattt atgattttat gaaaatgaat gatgttgcta ttttaaatgc agttgctaca
    26881 gcggaagggg ctatatcaga agctattaaa agaagcataa tcaatataca tcaaagtaac
    26941 tgcttagtat taggttatgg aagatgtgca caaatacttg caaaaaaatt atatgctttg
    27001 gatgcaaatg tatgtgttgg tgcaagaaat aatgatgcaa gaagtacggc tagtgcattt
    27061 ggatattcca gtatttcttt aaatcaactt gatgaaaaac ttttacaatt tgattatatt
    27121 tttaacacta taccagattg tattttgact aaagaaagat tagaaaaagt atcacaagaa
    27181 gtaactatta tagatatagc ttcttctccc ggaggagtag attattctgc tactaaagaa
    27241 gtagggatta atgcgactct ttgtcttggg cttcctggta aatattcacc aaagacatct
    27301 ggtgaaattc ttgttaacgc tatagaaaat attataaatg aaagaaggga ttagaaatga
    27361 gacttgaagg acttacaatt ggagtaggat ttactggttc gttttgtact tatgataaaa
    27421 tatttataga gttagagaat ttggttaaag aaggagctaa tgtccacact atattttcag
    27481 atgtatcaca aaatatagat tgtagatttg gaaattctga agagtttatg aaaaaagcat
    27541 atgaacttac aggaaataaa cctatagtta cgattgaaga tgcagagcca tttggaccta
    27601 agggaattgc tgatattata ataatagctc cttgtactgg aaatactgct gcaaaattag
    27661 caaatggtat tacagattca ccagtattaa tggcagcaaa aggtcatctt cgtaatgata
    27721 aaccactagt aatatcaata tcaacgaatg atgctctaag ctttaatttc aaaaatattg
    27781 gtattctcct taattctaaa aatatatatt ttgtaccttt tggtcaagat aattgcaaag
    27841 caaagcctaa ttctatgatt gcacatactg agctaataat tccaactata gaacttgccc
    27901 tagagaataa acagctacag cctgttataa aaagtccaca tcaatagaat tggttctata
    27961 atattatgta ttagatgcaa gactagcaaa gaggctgtct caaaatagag ataagcctct
    28021 ttttcattgt tgaaatgatg aaaatatatt taatttcata caaaaaaatg ccttatggac
    28081 taaaaagtct attttgagct aatctccata atttttggta tattaaaatt gtttacttaa
    28141 tctaattttt aaattagaat ttatatcgct ttgactcttc aaatatcaat ctaactgttg
    28201 ttgatacaaa agaagaggac ataattaaaa caaatactga tagtcctcta ataaacaaat
    28261 tatttacata ataagttact attccaatac ctccaatcgt taaaattaca cttataatca
    28321 aagaaaaaat gctaattatt tttaattttt tcatgattat cctccttctt ataaatttca
    28381 aatttatcta tataatatta aatatatttt atttcatagt atataaaaat catgaatata
    28441 tattttttta attatactat atattgtaac tttttgtata tttaactata tttactttaa
    28501 cttgttaatc aagttattat tttcattttc aagtattttt atttttaaac ttgatacagc
    28561 accatattaa aaatcatttt tataaagttt tatttgtaaa aaatacaaaa atataaacgt
    28621 taaaaaaata acgttatcct taagttttta gatgatttta ttagtaattt acgtaaaaaa
    28681 taaagtgaca tctagttaaa ttaagtattt gtaaagctag atattattag tataaatgat
    28741 gttatattag ataaaaaata aaatgttaaa aaaataactt gaaaatagaa aaagtgaatg
    28801 ctataatgtt aataaattaa cataataaat aaaaaactat ctcaggaggt tgttaaaatg
    28861 gctcataaag aacaagtaat aatagaaaat aaaaaagaaa atgaagttat gtcagttggt
    28921 ttagatactg taaataacgt tgaaagctta gttaaaaaac tagcaaaaat caaagaagct
    28981 caaaaaatat ttgctacata tactcaagag caagtagata aaattttttt agcagcatct
    29041 ttggcagcta ataagcaaag agttcctcta gctataatgg cacaaaaaga aactggtatg
    29101 ggaattgctg aagataaggt tataaagaat cattatgcct cagagtatat atataatgca
    29161 tataaagaaa ctaaaacttg tggtgttata gagaaagatg aagcatttgg tatgactaaa
    29221 attgctgagc caataggtgt tattgcagca gttgtaccta caactaatcc aacttctact
    29281 gctatcttta aagcactttt agctttaaaa actagaaatg gaataatttt ctctccacat
    29341 ccaagagcaa aaaattctac tatagaggct gcaaaagtgg ttcttgaggc tgctgtttta
    29401 gctggagctc ctgaaggtat aataggatgg atagatgagc catctttaga attaactaca
    29461 actgttatga aagaggtgga tttgactctt gctacagggg gtcctgggat ggttaaatca
    29521 gcatattcat ctggaaaacc agctattgga gttggtgcag gtaatactcc agcaataatt
    29581 gatgaaagtg cagatataaa aactgctgta aactcaattc ttgtatctaa gacttttgac
    29641 aatggtatga tatgtgcttc tgaacaatca gttatagtat tggaaaatat atataatgaa
    29701 gtcaaaaaag aatttaaaga gcgtggtgct tatttattag ataaagatga aacagaaaaa
    29761 ataagaaata taattttagt aaatggagga ttaaattcta aaatagttgg gcaaacagct
    29821 tgtactatag caaaacttgc aggatttgaa gtcccagtag atactaaggt tttaataggt
    29881 gaagtagaat cggttgaaat agaagaggct tttgctcatg agaaactttc accagtgctt
    29941 gcaatgtaca aggcaagtga ttttgatgat gcagttagaa aagcagaaaa attagtagaa
    30001 gatggtggat ttgggcatac atcttcatta tatattgatg atgtaaatca aagagaaaaa
    30061 cttgataagt ttacttcagc tatgaaaact tgtagaattt taattaatac tccttcttct
    30121 caaggtggta taggggattt atataatttt aaactagctc cttctcttac tttaggatgt
    30181 gggtcttggg gtggaaattc agtatctgaa aatgtaggag ttaagcattt acttaatatt
    30241 aagacagtag ctgagaggag agaaaatatg ctttggttta gagcaccaga aaaagtttat
    30301 ttcaagaaag gttgtttagg agtagcatta aaagaactta aagatgtaat ggataagaag
    30361 agagctttta ttgtaacaga tacattcctt tacaataatg gatatactaa agcagttact
    30421 gacttattag atgaaatgaa tattaagcac actacattct ttgaagtaga accagaccca
    30481 actttggaat gtgctaagat aggtgctaaa gctatgagag aatttaatcc agatgtaata
    30541 atatctatag gtggaggaag tgcaatggat gcaggaaaga taatgtggac attgtatgag
    30601 catccagatg tagatttcca agatttagct atgagattta tggatataag aaaaagagta
    30661 tatacattcc ctaaaatggg tgaaaaagct aactttgtag ctataccaac atcagcaggt
    30721 actggttcag aagtaacacc atttgctgtt ataactgacc aagatacagg ggtaaaatat
    30781 ccattagctg attatgaatt gatgccaaat atggctatag ttgattctga tatgatgatg
    30841 aatatgccaa aaagcttaac atctgcttct ggtatagatg ccttaactca tgcattagaa
    30901 gcatatgtat ctatgcttgc aaccgattat acaaatggat tagctttaca agccattaag
    30961 agtatatttg aatatcttcc acgtgcatat gacaatggtg caaaagaccc agaagctaga
    31021 gaaaaaatgg caaatgcttc tacaatggca ggtatggctt ttgcaaatgc atttttgggt
    31081 gtttgtcact ctatggctca taaattaggt gctttccacc atgtaccaca tggtgttgca
    31141 aatgctcttt tgataacaga ggttatgaaa tttaattctt cagacgcacc aaagaaaatg
    31201 ggagcattct ctcaatataa atatccagaa gcattaaaga gatatgctga agtagcttca
    31261 ttcttaggat taaaaggaaa ttctgaggaa gagaaattcc aaagtttatt gtcagctatt
    31321 gaagatttaa aatttaaagt tggtattcct aaaactataa aagaatttgg agttgaagaa
    31381 tctaagttta tggactcaat agatgaaatg gtaattcaag catttgatga ccaatgtaca
    31441 ggagcaaatc caagatatcc acttatgaat gaaataaaag atatgtattt aaattcatat
    31501 tatggaagat aaatttacta taaaatagga aaaataaaaa ttcttataca taaaacaaag
    31561 ttttattgta taagaatttt ttttgattgg aaaaatgtat agtttttatt ctttaattta
    31621 aaattcaatt aaatagtgtt attataataa taaaagaatg tttttatggt tattaggttg
    31681 attttatgtg taacatttaa gaatagttat tacaatataa atgatgaatt ggtataaaaa
    31741 ggatatatta tctatatgaa gtattgacta attatgggag gagtcgaatg acaagaagaa
    31801 gtaagagttt tacattaatt ataattcttt ttttgacatc attatttttg acaatgggat
    31861 atatttatat agaagataca aaaaatcttc ttatgtcaga agcaaaaata catataaaag
    31921 aagttgcaat acaaggctct caactagcac aaagacagat tgaaaaagat ttagatgttt
    31981 taaatatttt ttctaactat tatgcttcaa atccagacat acctaatgag gaaaaaatga
    32041 agaatctttt ggatgagttg gaaaatcaaa aattttacac tatggcaata gttgatataa
    32101 atggtaatgc tgaaaatacc aaaggtgata agttttcagt gaaagataga gaatttttca
    32161 aaaactcaat taaaggtaaa aagtatgttt caagtccata tgctgatgaa gtaaataagt
    32221 ctataaagaa gattactatt tcagtgccat tattaaataa tgataaagta gttggagttt
    32281 tatattgtac ctacaatatc aatactttga tgaaacttat taatgtatct ttttatgaaa
    32341 ataatagtat atcttatgtt gtaaaaaatg atggtacagt tattcttcat ccacaagagg
    32401 atagcatatc aaaaaatatt tataaattgt taaaacagga taatgatatt cgagaagtaa
    32461 atagcttaaa aaaagaactg caagaaaaca aaacaggtgc aactgtatta aagatgttgg
    32521 aagaaagaag atatttagga tatgctacta tggataatgg gaatagtgaa aataactata
    32581 taaaagattg gaatttgatt ttttcaattc cagaaactgt tgtgtttagt aattcagaaa
    32641 aaataatcaa cagagctata tatgcagtgt tatttattat attaatattt attgcaatta
    32701 ttttttatat aatttatata aaaaaatcta atgaaaaaga aatattaagt ttggcttatg
    32761 aagataaagt tacccatata ggaaatcaaa ataaatttta tagagagtgt agtaagtatc
    32821 ttttagataa accatcttta aattatatta tagtatattt tgatattaat aactttaaga
    32881 tgattaatga tacttttgga tatgaatttg gtgacaactt attaattacg atagcaaaag
    32941 ctttaaaaga agaactgaca gaaggggaag tatttgcacg actttcaagt gattattttg
    33001 caattttctg tgattataaa aatggaagaa ataaaattat aagaaaactt gatagtatac
    33061 gaaataatat tgaaagtaat ttgagtatag tctttgaaat ttctctgtgt gtgggtattt
    33121 attttgttga agaagatgaa gtagatattc aaaaggctgt aaataaagca aatatggcta
    33181 ggtctgttgc taaaggtaaa aatataaatt atgctattta taatgaagat gttaggaata
    33241 agcttagtga agaatcaatg attttagatg atataaaaat agcattggta aaaaatcaat
    33301 ttgaagtgta ttatcaacct aagttttcac ttgtaaatgg agaaatgata gggagtgaag
    33361 cactaataag atggaatcat ccagaacatg gttttataag tcccgcagta tttattccaa
    33421 tagcagagaa aagtaagttg attttaaaaa ttgggagatt tgtatttgaa aaggtttgca
    33481 ctgatttatc tgaatggaaa aaacaaggta aaaagatagt tccagtttca gtaaatttat
    33541 ctagagttga attgtatcaa ccagatattg taaaatttat caataaaaca atacaaatgt
    33601 ataatctaag ttcagattta attgaaatag agataacaga aactgtggct attaatgagt
    33661 taaatatttt aaagaatgtt ttaaatgagt tgaggaagta tggattttct atttctatgg
    33721 atgactttgg tacaggatat tcttctatta gttgtttaag agatatgcca attgatatat
    33781 tgaaactgga taagtctttt cttgatggta ttgaacatga tgagagaagt cgcaatatag
    33841 caaaatcaat agtgtctcta gcaaaatcac tagatttagt tgtaattata gaaggtgttg
    33901 agagtaaaga acaagctgag ttaatgaaac aatttggatg tgatttagtt caaggatttt
    33961 attttgcaag accaatgcca gctaagaatt ttttagattt actttgaaat aaaatttgat
    34021 ttttatataa agcactatta tgaatgcttg attttaataa atcactgcta aataattagt
    34081 gtttttttat tttatcaatg tattatctaa tataaggtaa aaaataattg tattttataa
    34141 aaattatatt aaaaataaat ttattttgct aataaacatg tcattaacaa aagaatagta
    34201 ctataatgta tagatgataa aaactttaat aaaaatttag tgtataggag ttgtttaagt
    34261 atgatagatg taagtattga taaaaaactt aaggaaagat ggcctaatgc taagctgggt
    34321 tgtattcaag caaaagtagc agtaaataaa agctcagaaa aattaattaa tgaaataaat
    34381 gagttttgtg atactctaaa taaaaatcta aaagttgaag acattacaaa actagataaa
    34441 attaaagatg ccagaaatgc atataaagaa cttggaaaaa atccaagtag atatagaacg
    34501 tcttcagaag ccttggttag aagaattatt caaggtaagg gtctttacac aataaacaat
    34561 atagtagaaa taaacaattt aatatcaata aaatcattat atccagtagg tacatataat
    34621 gtaagtaaat tacattcacc agtatgtttt actgtaggag atgaaggaga acagtataag
    34681 ggtattggga aagaattaat aaatatagaa aatcttccta tattgtctga ttcagtgggt
    34741 aaatttggga gtccaactag tgattctgaa agagcaatga taacagaaga tgcgaatgaa
    34801 atattaattt gtatattttc ttttagtgaa gaatctgata ttgaagaata ttttggatat
    34861 gctaaggaat tacttaaaga atacgctgat ggaaaagata tagaaactaa aataattaaa
    34921 tagaatccat ataacaacgt tatgttaagc taaaattcaa tataaaaata agttatgata
    34981 aagaaactaa cacaaaatta gtttcttttt tgaaagagta gtttagtgtt acaaaaatag
    35041 atagatatcc ccacgagtat taaaagtatg gtaatatatt aatatagaag ggattgtatt
    35101 taagggattt tgaaaggagt gatttataat aactgttttt tctaaaagat aaaatggttt
    35161 tatggaaata actatatatt taaaaatata aataataata cagtgatttg ggtatataaa
    35221 ataagacata catggttttg tttaaaacca ataaagtaca taggtaatta caatgtaagg
    35281 aaggatttaa ttatgattga tggaaaagag gtaaaacaag aaagtggaca aaaaaatcta
    35341 aaatttaaga tagctatagt agtaggtatt ttatttgtag cttatatagg aatggcaatc
    35401 ttttttagaa atcattttta ctttggaacg actattaatg gtgtaagggc ttcaggaaaa
    35461 acagttgaac aagtaaatga tttgttaaca tctagtacta attcctatac tttaaactta
    35521 gatgaaagaa acaataagaa agaacaaata aaagcaaaag atattgattt aaagtttaca
    35581 ccagatgata aagttcaaaa gataaaagat agtcagagtg ctattggatg gattggtggg
    35641 atatttaagt ctaaagagta taacaatatg gtaactcttt catataataa agatttacta
    35701 gaaaaaagat ttaacgctct atcttgcctt gatagtaaaa atgttgtaga gcctaagagt
    35761 gcttatccta agtatgatag tactaaaaat gcttatgtta tagtgccaga ggtacttgga
    35821 aacaaagtga aaaaagatga tttttataag ttagtacaaa actcaattaa ttctggtaat
    35881 acttctattt ctttagatga aagtaaagtg tatgaagtgc caaataacac aaaggattca
    35941 gaaaagttga aacaagctat taagacacta gataagtatg ttggtgtagt tgttacatat
    36001 gattttagtg accgtaagga aactttagat ggttctgaga ttaataaatg gttaaaggta
    36061 gactcagaaa agaattatga tataactttc gataatgatg ctatggtaga gtatacaaga
    36121 ggtttaagta aaaaatatag tacttttgga gattcaagac catttaaaac tgcaacaggt
    36181 aaaaatatta caatttcagg tggaatttat ggatggttga tagacaaaaa gaaagaagct
    36241 gaagcattag ttgatgttgt aaaagctggt aaaaatgtta caagagaacc tatttacata
    36301 caaaaagctg tatctcgtaa tagagatgat attggaaata catatgtaga gattagtctt
    36361 tctaaccaac atatgtggtt ctttaaaaat ggagaaatgc ttgtatcaac agacattgta
    36421 actggtaatt tagctagagg atttgctaca ccatcaggta tatatcctct taattataag
    36481 gctagaaatg tttcattaac tggtcaaggt tacagttccc ctgtaagtta ttggcttcca
    36541 tttaatggaa atataggtat tcatgatgct acttggagaa attcttttgg tggaggaata
    36601 tataagtctt caggttcaca tggatgtgta aatacacctt tttctaaggc aaagaaaata
    36661 tatgaaaata tagaaccagg tacaccaatt attttatact agtaaatgtg ttactaacag
    36721 ttaagcgaag tcatcttagt ttatttaggg tggcttcctt tttatataaa gtttattgaa
    36781 gtttacataa agactattta aaaggagaaa tacataatga atgaaaaatt aactaagatt
    36841 ttagagaaag ctaagaatga tgatgaattt agaaaagaac ttataaaatc aagagaacaa
    36901 cgtgatgtta tggattcatt ttgtaaggtt gttacatcta gaggttatga tataacagta
    36961 ggggaacttt tttccatagg agaagaatat acatcaaatc tacttaaaag tgtgaatgga
    37021 ggggcaacat atcctattga agattggagt gattggtacg aggatttttt tataatgttg
    37081 tcgaatatat agagtaacta atgttattat ctacttaaga ttaacacctt aattttaaaa
    37141 aaaactatta gagctaagtg taaattttat caatctttaa aaatattgat ttatttctag
    37201 ctatatataa aaaaattaga ttaaaaataa ggtgacaaaa atcgatatac cttgatgatt
    37261 tataaaagtt agacaaaata gtgctagata tttctaaaaa ttcaaaaaaa acaataaact
    37321 tcaagagcta gaggttgaaa aataaaataa aaaactttta tatgtaataa attaaattga
    37381 aattataaaa aaaataaaaa agaaatttta aatgtcgaca tagcgttaat tttaagacaa
    37441 aataacgcta ttgacaaact atatcgcttt aaattataat tattgaaaat aatggtattt
    37501 aaaaatcata ctcatgcaat agtctaaaag taagtcaaaa aaaaatgtaa atctaggcaa
    37561 gtactattct aaaatatgtt ttattaagaa ataaacgttg aaaaatatta attattatat
    37621 aaaaatgctg tttatacttt aacaatgagg tagtaatcta attagaataa aaaaatctaa
    37681 ttggttatat acatagtaaa aaggtaagag aggtgatttg gatggctttt gaaataataa
    37741 aaagcattgt tgaggcagag cagacagcag acagtatcaa agtaaaagct gttactgatg
    37801 cagagtctat cagagctgat gctgtaaaca aatgtgaaag catatttgct gatgtaaaaa
    37861 aacaagcaaa gcttatggaa gaaactctta ttgagaaggc agtcaccgac agtagagcag
    37921 aggttgataa aatcttagct aatgctaaaa gtgaatgtct gaaaattgaa aaaactgcta
    37981 aagaaagaaa aagtaaggct attgaagcgg ttattggaaa ggtagtgaga taatatggca
    38041 atagttaata tgaacaaaat atctattgtt ggcttagaag ctcaaaaaag ccagatttta
    38101 aagctactta tgaaccgtgg gtttgttcag attgatgata gtgcctttct tactgaggag
    38161 gaggaattaa aaaattatct tgtaaaagat agtgaagaat ctgaagttat aatgctagag
    38221 cagaagatgt ctcatgtaaa ccaagcaatt aatattgttt cttcactagt gaaacaaaaa
    38281 aaatccctac ttgcaccaaa aagagagttt aacaaaattg atagtgaaaa agaagagcaa
    38341 gtttatgctg aagctcttga gttaaacaat ttacacaagg agataggtac aataaggtct
    38401 aacataaact tacttaacaa caataaaagt atgttagaac cgtgggtaaa ctttgatatt
    38461 cctcttgaaa atatggaaac taagtataca aaaactatac ttggaactgt acccataagt
    38521 gcaaacattg atggtttaag agtaaaatta gaagatgaaa taccagaaag tgttattggg
    38581 attgtcaatt ctgacaagtt aatgaactat gtttatttaa tcactttaaa agaaactttt
    38641 gatgatgttt tagaagtttt aaaagaattt aatttttcag tggtttcatt gccaaatgaa
    38701 gaaggtacac cgttacaagc tatcaagaaa tacaatgaaa tgataattag acaggaaaac
    38761 gtttctaaag acaaaacaga ggaaattaaa agacatgcag agagtataca catgcttgaa
    38821 aatctatatg atttttacac tatagaaaga gaccaaaaaa agattataga gagacttgtc
    38881 aaaacaaaaa caacattctg tcttaatggt tggttaccaa gcaaaagggc agatgagcta
    38941 attaaagaaa ttacagaaaa gtttgattgt tgcgttcaga ctgaagaagg aagtaaagaa
    39001 gaaggcttcc caattttgct tgaaaacaat aaactagtaa caccttttga aagtgtaact
    39061 aacatgtaca gctatccaag tacaaaagat attgacccaa atacaatttt aacattcttt
    39121 tttgttgtat tctttggaat gatgctaagt gacgctgcat atgggttaat aattgcacta
    39181 gtctgtggat ttgttgtata taaattaaag atacaaaaag gtgaaggaaa tttaattaaa
    39241 cttattggta tctgtggagt ttcaactact gtatgggggc ttatattcgg gggtgttttg
    39301 ggggatttaa ttcctataaa agcattaatt aatcctttag aggatgttat gacacttatg
    39361 ggaatgtcgt tactgttcgg tattatccat atatatgtag gtttgggaat aaaagcatat
    39421 acattaataa gaagtggaaa ggtaatagat gcaatctttg acataggttt ttggtatttg
    39481 tgtataacag gtgtatgtct acttataata ccttttgttg caggggacat aggtgtattt
    39541 tcagaagtag gaaaatattt agcaattata ggtgctatag gtcttgtact tactcaagga
    39601 agaagcttta aaggtattcc tgttaagctt tttaaaggat tttcaagtct ctatggcata
    39661 acaagttatt ttgctgatat attatcttat acaagaatta tggcactttg tttaacaaca
    39721 ggagttatag cacaagttat caacctttta ggagctatag ctggtccgat acttgcagtt
    39781 gtaatcggtg ttgtaggaca cacaataaac cttcttataa atgcactagg tgcatatgtt
    39841 catacaagta ggttacagta tgtagaattt ttcaataagt tttatgaagg cggaggagtt
    39901 ccttttgttc cgtttaagta taaaacaaaa tatacaagca ttaataaaaa ggagatgtaa
    39961 atatggaatt tttaaacgcg ttaggaaatg gtcaattttt ggcaatatca ggagctgcta
    40021 tagcagcatt atttgcaggt atgggttctg ctaaaggtac ttcaatagca ggtcaagccg
    40081 cagcaggtgt tgtaacagaa gaccctagta aattcggtca acttctagtt ttacaacttc
    40141 ttcctggtac acaaggtttg tatggattta tcatagcttt ccttatttta tcaaaagcag
    40201 ggattattgg tggagatgca ataccaacat cagctcaagg tttacaactt cttatggcag
    40261 gacttccaat tggtttagtt ggtttagtat caggtatagc acaaggtaaa gcagcagcag
    40321 caggggttgg aatagttgca aaaagacctg aagaacttgg taaagctata acatttgcag
    40381 caatagttga aacatatgct cttttaggac tacttgtttc tttcttagct tacaatggaa
    40441 ttgcaattgg ttaattatag attataattc ataagtaaaa aaaatgaaaa ggaggaagca
    40501 ctcctatggg taatgaacaa aaaatgattg acagaattat agctgatgcc aaacaagaag
    40561 cacaagaaat attggataag gctaaaagtg aggcagattt aaaagtaaat tcagctaatg
    40621 aaaaagctga aaaggaaatg gcttcctata caaagcttgc agaggcagaa gcagaaaaag
    40681 cagcttctaa ggaaatatca ggagcttata tggaggctaa aaaacaaata ttatcaaaaa
    40741 aacaagagat tttagaagag gtaattttag aagctaaaaa caaacttcta aatcttaaag
    40801 ataatgaata tgaagagatt atactaaaca tgattgaaaa atcaaattgt acagatgatt
    40861 cagagatagt attatctaaa aaagataaaa aaacattaaa agatgtgcta tcaaaaaaag
    40921 gcattaaagt gtctgatgaa actagagata ttactggagg tttcatagtt aaaaaaggtg
    40981 acattgagta caattattca tttgaagcca taattgcagt agagcatgaa tatattgaac
    41041 aaattgcagc agaaattttg tttaattaag gaggggttaa aaaatggcag aagaaaaaac
    41101 ttatccctat gccgttgcac gaataagagt gcttgaaaaa cagcttctta acagacaaat
    41161 gtttattcaa atggcagaag ccaaaagccc tgaagactct ttaagaatta tagctgaggc
    41221 aggttatgct gatacatcaa ataataatat acataatttt gagaatattt tgactcaaga
    41281 attatcaaaa acatatgaaa ttcttaaaag tctagctcct gaagaaaaat ttgttgatgt
    41341 gtttctttat aaaaatgact atcacaattt aaaagtgctg attaaagaag aaatttctgg
    41401 tgtagatggt gagaaatatt taattgatgg aggtacaatt cctcttatta agcttagaga
    41461 atctttagca aatcgtagtt ttagtgattt acctaaggta atgtcatcag ctatattaga
    41521 ggcatttgat gtttatagta agacacaaaa tggccagatg gttgatattg ttctagataa
    41581 agcgactttt actagtatga aagaaactgc taaagaaagt caaaacaaat ttgtaataga
    41641 ttatgttatg aaagtttgtg atttaacaaa tctaaagagt tttataagag tcaaaaatat
    41701 gaagaaagat tttgatatgt ttatgaatgt gtttgtttca ggtggctcac ttgataagga
    41761 aaaattttta gaagcttttt cctcagatac tccagcatca tgttttaaat ctacttctta
    41821 ctctgatgtt tgtaaaaatg gcatggatag tggttttact gtctttgaaa aactttgtga
    41881 tgactatctt atggaatatg ttagaggggc aaaatttaag ccacttacat tagaaccact
    41941 aattgcatat ttatatgcta aagaatcaga aataaaaaca attagaatta ttttgactag
    42001 taagcttaat aatatagacg cagatactat aaaagaaagg ctaagggatg cttatgtata
    42061 aggtaggagt agttggagat aaagatagca tcatgggatt tttagctctt ggaatagata
    42121 tcttcccagc ctatgatagt gacgaaatta aaaaaagtat acataagttg gttgaagatg
    42181 aatatgcgat aatttatatt acagaacagg catcattgct tgcaaaagaa tcaatagcaa
    42241 aatacaaaga ttaccaactt cctgctatta tagttattcc aggtattgga ggaagtatgg
    42301 gactaggtat gaatgaagta agagaatcta gtaaacgtgc tataggtgca gatatattat
    42361 ttaaagaata gatgaaagtt gaataaaaga caatctcaaa ttttattggt attaaagata
    42421 tctactaata aaatatagga gggaatattt aaattttgat aaagtatatg attgcaggtg
    42481 agagaatatg aaaacaggca caattgtaaa ggtgtcagga ccgcttgttg tagcggaggg
    42541 catgagagat gcgaatatgt ttgacgtcgt aagagtttca gataaacatc ttataggcga
    42601 gattatagaa atgcatggag ataaagcttc tatacaggtt tatgaggaga cttctggtct
    42661 aggaccaggg gaagaagtag tttctcttgg aatgcctatg agtgttgaac ttggaccagg
    42721 tcttatttca actatttatg atggtattca acgtccactt gaaaaaatgt atgatatttc
    42781 aggtactaat ataactaaag gggttgaagt atcttcactt gatagaacaa ttaagtggga
    42841 atttaaacct aagaaatctg ttggtgataa ggttgtagca ggagatatca ttggtggagt
    42901 tcaagaaaca gcagtagtag aatgtaaaat tatggttcct tatggagtta agggaactat
    42961 aaaagaaata tattctggtg aatttactgt tgaagataca gtttgtgtta ttacagatga
    43021 aaaaggtaat gatatacctg taactatgat gcaaaaatgg ccagttagaa aagaaagacc
    43081 atatagagaa aagaaaacac cagattcact tcttgttaca ggtcaaagag ttatagatac
    43141 atttttccca attacaaaag gtggggtagc agctatacca gggccttttg gaagtggaaa
    43201 aactgttaca cagcatcagc ttgctaaatg ggcagatgca gatatagttg tatatatagg
    43261 ctgtggcgag cgtggaaatg aaatgacaga tgttcttctt gaatttcctg aattaaaaga
    43321 cccaagaaca ggcgagtcac ttatgcaaag aactgtgctt atagcaaata catcagatat
    43381 gccagttgct gcacgtgaag cttctatata cactggtatt acaatagctg aatattttag
    43441 agatatggga tatagtgttg cacttatggc agactctaca tcaagatggg ctgaggctct
    43501 tagagagatg agtggtcgtc tagaggagat gcctggtgaa gaaggttatc ctgcatactt
    43561 aggttcacgt cttgctcaat tctatgagag agcaggaaag gtaaattgtc taggtatgga
    43621 tgaaagagaa ggaacactta cagcaattgg tgcagtttct cctcctggtg gtgatacatc
    43681 agaacctgtc agccaagcaa cacttcgtat agtaaaagta ttctggggtc ttgatgcatc
    43741 tttagcatat aaacgtcatt tcccagcaat taactggttg actagttatt cactctatca
    43801 ggaaaaagtt gatgtatggg cagataaaaa tgtaaatgat aatttctcag ctcaaagagc
    43861 tcaagctatg aaacttttac aagttgaatc tgaacttcaa gaaatagttc aattggttgg
    43921 tgttgactca ctttcagatg gagatagatt gatactagag gttactcgtt ctataagaga
    43981 ggacttcctt catcaaaact ctttccatga agttgatact tatacatcac ttcataaaca
    44041 atatagaatg atgggactta tattaaaatt ctataagtca gcacaagatg ctatcaagca
    44101 aaatgttact ataaacgata ttatcaagct ttcagttctt gaaaagattg gtcgtgctaa
    44161 gtatgtagag gaaggcgaca tagatgcagc ttacgattca attgaagatg aaatagacaa
    44221 cgagcttgca gaccttatta aaaagagggg ggctgaagaa ctatgataaa ggaatataag
    44281 tcaatacaag aggtggccgg tcctcttatg ctaataaacg gcgtagaagg tgtaaaattt
    44341 gatgaacttg gagaaattga actttcaaat ggagagattc gccgttgtaa agttctagag
    44401 gtaaacggag atactgcact tgttcagctt ttcgaaagct caacaggtat aaaccttgct
    44461 gaaagtaaag ttcgtttctt aggaaagagt cttgactttg gagtttcacc agatatatta
    44521 ggtcgtgttt ttagtggaat gggtagacct attgatggag gtccagaaat tataccagat
    44581 aaaagacttg atataaatgg agctccaata aatccagcag cacgtgacta tccggctgaa
    44641 tttatacaaa ctggagtttc tgccatagat ggacttaata cactggttag agggcaaaaa
    44701 cttcctatat tctcagggtc tggtttgcca catgccaatc ttgcagttca gattgcaaga
    44761 caggcaaaag tacgtggaac tgattctaag ttcgccgttg tatttgctgc agttggtata
    44821 acttttgaag atgctgaatt ttttataagt gatttcaaag caactggtgc aatcgaccgt
    44881 tctgtagtgt ttgtaaacct tgcaaatgac ccagctgtag agcgtgtatc tacacctaga
    44941 atggcactta cagcagcaga ataccttgct tttgagcaag atatgcatgt acttgttata
    45001 ataacagata ttacaaacta tgcagaggca ttgcgtgaag tttcagcagc taaaaaagaa
    45061 gttccaggac gtcgtggata tccaggatat ctttatactg accttgctac tatgtatgaa
    45121 agagcaggaa aaatgaaagg gcatgaaggg tctataacta tgattccaat acttacaatg
    45181 cctgaggatg ataaaacaca tccaattcct gaccttacag gatacattac agaaggacaa
    45241 attatactta gccgtgaact ttataaaaaa gatatacagc ctcctataga tgttttacca
    45301 tcactttctc gtctaaaaga taaaggtatt ggtaagggta agactcgtga agaccatgca
    45361 gatacaatga accagctttt tgcagcttat tcaagaggta aggatgcaaa agagcttatg
    45421 gctatacttg gtgaatctgc actttctgaa attgatttaa tgtatgctaa gtttgcggat
    45481 gaatttgaga aagaatatgt ttcacaagga tttagaactg atagaactat tgagcagaca
    45541 ttagaaatcg gatggaagct tcttagtatg ttaccaaaaa gtgaacttaa acgtattcgt
    45601 gatgaatatt ttgaaaaata tatgcctaag gagtgatatc tgtggctaga ttaaatataa
    45661 atccaacacg tatggaaatg actagactta agaaactcct taaaactgct acaagaggtc
    45721 ataagcttct taaagataaa cttgatgaac ttatgaaaca atttcttgag attgtaagag
    45781 aaaataagcg attacgtgaa gaagcagaaa atgctcttga tgctgcttat aaaaatttta
    45841 taatcgcaag agcagttatg agtcaggagt atctgggttc agcacttatg atgccaaagc
    45901 aatctgtttc agtagatgtt tctactagaa atataatgag tgttgatgtt cctgtgtttg
    45961 actttaaaac tgaaaacaat caaagtgata tttatcctta tggtcttgct tttacatcag
    46021 gtgaattaga ttctgctatg gaagctttct cagctgctat gcaaccactt ttaagacttg
    46081 cagaaagtga aaaaagtgca cagcttcttg cacaagagat agaaaagaca cgtagacgtg
    46141 ttaatgctct tgaaaatgta atgataccaa attacattga gactattaag tatatagcta
    46201 tgaagcttga agaaaatgag agagcaagta ctactagact tatgaaagtt aaggatatgg
    46261 tgcttaaaaa agcacttgaa gaaaagaaaa aaaatgattt ggttgtataa ttaaatactt
    46321 ataaaaataa gtctaacatt ctaatttata gaggttaggc ttatttttat gtttaaatta
    46381 aaagtgtaaa tagtataaaa atcaatacat tgtttaaaaa cagactgtat ttaactaaaa
    46441 ataatatatt taacaaaaat ttctaaaaat taacgaactg ttaatttaca cttaacttta
    46501 tattaacaaa ttaggtgtat tatataaaca taagcaaaaa caataaaaaa caaataaaaa
    46561 aagaaaaaaa cacaaagggg gaagatatga aagaatttat atttacttgt ctgtgcattg
    46621 tagtgattac atttatgaca agttgcatat caacaacacc acaaactact caaggaaaag
    46681 gaaataataa acttacaata tatgcagcac tagaggaaga gcagatagct tactatatgg
    46741 aaggttttag agaagagtat cccaatatag atgtagagat agttatggat tctcatggtg
    46801 taatagcttc aaaattatta gcagaaaaag ataatccaca agctgatgtt atatggggat
    46861 tatcagcaat gaacatgata gaactaaaag aagatggtgc acttgaagca tataaaccta
    46921 aacactatga taaagttgat aaaaaattca aagatactga agatgatgta tcttgggtag
    46981 gaatgagtgt cccggaaaca gcaatagtag ttaattataa ggaacttaag aaactaggta
    47041 tagatattcc aaaaagttat gaagatttaa taaaaccaga atataaagga cttataacaa
    47101 tgccaaatcc agcatcatct ggaacagggt atcttacagt atcaggcctt attcaaatga
    47161 tgggtgcaaa agatgcttac aattacatgg ataaattaca taaaaatata gctacatata
    47221 cacactcagg ttcaaaacct gcgaaactag ctggttctgg agagtatcca ataggtatta
    47281 cacttggata tagagctaca caacaattat ccagtggaga acctgtagaa gttgtatttc
    47341 caaaggaagg ttcaggttgg gacttagaag caaatgcctt agttaaaaaa gataatataa
    47401 aaaaagaagc aaagttattt ttagattggg caataagtga ttcagctatg gatagatatt
    47461 cacatgaagt agcagttact tccataaaga cagataatcc agttccaaaa ggttactcta
    47521 aacaaccatt tgaacagctt ataaaagaag ttgatttaag ttctgcggct aaaaacagaa
    47581 gtacagtact taaaacttgg gaatctaaat atggttcaaa atctgaaagt aagtaaagtt
    47641 taaattaata atttagatga aagaatttta gaaaaaaagg taggatatag aatttaagat
    47701 aaaaatataa ctatataagg agagataaga tatgagttat ttaaaaataa agaatgtgtt
    47761 taaaaattat gaccaaaaaa gagttttaaa taatattagt ttggatattg aagaagggga
    47821 atttctatgt ttacttgggc ctagtggatg tggtaaaact acattactta gaataatagc
    47881 aggtcttgaa gatgtaaata gtggagatat aattcttcag gataaagata ttacaaattt
    47941 agaaccatca aagagaggtt ttggtatagt atttcaatca tatgcattat ttccgaatat
    48001 gactgcttat aacaatatag cttttccact aaaagagaga aaaatttcaa aagaaaaaat
    48061 agataataaa gttaaggaag tacttgaaat agttgggttg accaatgaag ctcataaata
    48121 tcctaaagca ttatcaggtg gacaacaaca gaggattgca atagcaagag cattagcact
    48181 tgagccgaag tttttacttt tagatgaacc aatgtctgca ttagatgcaa aggttagaca
    48241 caagcttaga atggacatta aaaggctaca aaaagaactt aatataacga caataatggt
    48301 aacacatgac caagaagaag ctataactat ggcagacaaa atagctattc taaatggagg
    48361 agatataatg caaataggaa ctccagagga aatataccaa aatcctcaaa acctatttac
    48421 agctcaattt ataggagata caaactgttt tgataatgga gattctatat taactgtaag
    48481 accagaatat gttcaaatag agaaatcgac aaaagaaaat taccaaggta taatatcaaa
    48541 tatagagttt agagggaatt tattaagagt tgaaataaaa gataaactaa atgaaaattt
    48601 cataataagt gatgtttcta taaaagagtg ggttaattta aatttagtag aaggtgacct
    48661 tgtaaagata agtatagacg aaaaatacta tttaaagtat ccaaaagtta aaggagcata
    48721 aaggtatgaa taataatgct ataggttata aaagtataag tcttaaatca aaaatgaatt
    48781 tagatataaa aaaatgtatg cttacagtat tagttatgtc tttagtaata tttttattgg
    48841 cacctataat atatctattt gtacaggctt ttttagataa agatggagtc tttgtaggat
    48901 tacaaaactt tcaagaatac ttggcttctg aaactctatt aaattctcta aaaaatacaa
    48961 tatttgtttc agttatatca actgtaatat cactagcact agcactagta tatgcatatg
    49021 cattaactag aacaaatctt aaatttaaag gtttttttaa atcattagca atgtttccta
    49081 tgtttgctcc aacaatgatg catgcaatag gtctagtata tttatttggg aatcaaggat
    49141 tggtaactaa tttacttgga atacatgttc caatttacgg agcagtagga ataatactgg
    49201 cacagattat atatgtattc ccacaatcat ttcaaatact ttatatatct ctaaattcaa
    49261 ctgattatag actatatgaa gctagtaata ctatggggat aagcaagata agagaattta
    49321 taagcataac aattccaagt ataaaatact ctttaataag ttctgcattc gtatcgttta
    49381 cattatgctt tacagatttt ggagctccaa aggtaatagg aggagagttt aatgtattat
    49441 caatagatgt ttataagcaa attatagggc aacagaatat gccaatggga gcaactgtat
    49501 caatattact tttaatacct gcaataatat cgtttatagt aactaaaaaa attgaaaaaa
    49561 atcaaaactc atatataact tcaaagtcaa ttccatataa ggttaaaact aataagttaa
    49621 gagatagtat tttaggagga attgtaggaa ttattgcatt tggtataata tctgtaatgt
    49681 taactgtaat cttatcatca ttaatatcag tttggccata tgatttaagg cttacactgg
    49741 aacacttttc tatgaaaaat agtattgatg gaattaaacc atttataaat tcaataaaaa
    49801 tgtcattatt gacagccatt ctagggacga gctttgtttt cttatttgca tatttaaatg
    49861 aaaaaacaga aaaagccccc tatttaaaat catttgggta ttttttaagt acattgccta
    49921 tggcattgcc aggattagtt ttaggtatag cttatgtatt attctttaat aaattggagt
    49981 ttgattttat ggatacaata taccttaaaa atctgtttaa tggattatat ggaacattgc
    50041 ttattatggt tatatgtaat atagttcatt tcttttcagt agcatttata acagctacta
    50101 ctgctattaa gaaagtagat aaagaattgg atatggtagc taaatcaatg ggaataaaca
    50161 gcgcatcaat tcttaagaat gtaactatac cactttgttt gccagcagtt ttagagaact
    50221 ttatgtactt tttcctaaac tctatggtta caatatcagc actagttttt atatatactg
    50281 caagttcaaa agtagctgca atatcaattg tacaactaga tgataagggg agtactgcac
    50341 aggcagcagc actagctgtg ttaatattaa tcacaaatat attagtgaaa gcaatacatg
    50401 aatttataaa gaatcgttta gaaaaaagaa caaactttaa ttaaagaaat tattctacaa
    50461 aattataaat ttaaaaagtc tattctcaca atttattatt tacagaatag actttttaaa
    50521 tttattatta atattattta ttaattttcc ttttgttatc atacatttga gaatctgcat
    50581 aattatactt ataaaataaa gaaatattta ttaaaatttt ctgtaaaatt tttagtttta
    50641 ctggaagatt agattcagaa tattatcaag ctaagtttga ctaattatta agtttcgcat
    50701 caaaacaatg ttttaatagt gtttacagtt gaagtttttt ataaaaagtt tagttcccgt
    50761 ttaaaattta tatttatttt gatattttct taatttataa tatattatat taatacatag
    50821 ttattagaaa taagacacaa ttaaataatt atataaaatt gggaggttaa aatgggaagg
    50881 gccaaatatt atgatatata tatagattta aaaaatagaa tagaaaacaa ggaatattta
    50941 taccaagatt tattaccatc agaatacaca ttaatagaag agtataattg ttctagaaat
    51001 acaattagaa gagctataca ggaattagca aaccaaggat atgttcaaag taagcatgga
    51061 aaaggagtat ttataattta caaggctaac aagcaatcag agtttttatt aggaggaaca
    51121 gaatcattta aagaatctgc tattagaaac aagaaagaat ataaaacgaa agtagttcat
    51181 tttgaggaaa tggttataga tgataagaaa agtcaaataa ctaactttga agttgggaca
    51241 gaagtgtatt atatacaaag agtaagaatt ctagaaaata aaccatctat tatagatcat
    51301 aattatttta tgaaagatat agtaaaagat attaatgaag aaatagcatc taattctata
    51361 tatgaatata tggaaagtga attaaatgag actatagtaa caacaaaaag aactatgaca
    51421 gttgaaaaag caactaaact agataaaaag tatttagaat taggagatta taattgtgtt
    51481 gcagttgtta gcaataatac ttataatgcc caaggtataa tgtttgaata tacgcagtct
    51541 agacacatac ctgatggatt catatttttt catcaatctc aaagaattaa taatataaaa
    51601 tagagtacaa tttgtactct attttatatt attaattctt tgagagttat actcataaac
    51661 tttagcagct aagtatatga atgtactagc tgtccaggtg aaagatttat ctctaagacc
    51721 ctctcctgta atagcgttga aattttcagc aaagccgttt ttatcaacta attcacaata
    51781 tcttttggca atatctattg caagttcatt ttcaccacat tcttctaaag cttcaacaaa
    51841 taatagtgta gtaggagccc aaataggccc tctccaataa gcatcatctt gatattcatc
    51901 actatctata gcttctgttg cgattcccca tttagttaaa tatctttcgc tagtgactaa
    51961 atctatcatt ttgtctctaa tatatttagg aagtctattt cctaaaacta aagaaacaag
    52021 aggaagtaaa gcgttgttag aaagtatttc accagttaca gtttcgcgag ctacaggtaa
    52081 atcatcaact ataaaataat ttaagaataa ctttgtaagt tcatctgctt tattagacca
    52141 atactctttt tcactatttc tgtttagttt ttcagctact tttgatagca tgtccatagc
    52201 atttattata aatgctgtta aatcaggagt atcaacaacc atagctcctt taaatattgt
    52261 actattatcc tgtccagaat catttccatg gtgatattca aaaataccat ccatatttga
    52321 atctttaaac tttacataaa acaatacttg tttttctatg taattataaa ttttttctaa
    52381 tgtttcttta gtaaagttaa tttttttcat catcttacta aaaaataatc cttgaatagg
    52441 tggttttgta aaattccatc taatagtaga atcgcttaag gacccaggaa tttgaccata
    52501 ttcatcttgg tagtcgaaaa gtactgccat ttggtcaaaa gctaatttat catttacacc
    52561 agctaaagct aatgcgttaa aacagtgatc ccagctccat gcacctaaga atgttgagtt
    52621 tgaagcaagt atagtatatc tctttaggta tccttgaggt ttgacaaaag aagaccaatt
    52681 tatataactc gcttttaaaa gtgagttttt atatttttca tctactttag gttgagctag
    52741 ataatattca tcaaataatt tttttgtgct atttagtgaa gtttcaaagt tatattcctt
    52801 gtaatctgga actttcatat gagtaggaat gtcttctata acacatataa attcattatc
    52861 actattactt ttaacatcta ttattgaagt attattagct ttatttgtag aagcacagtc
    52921 atctataaat atattttgtg ataagccaac cttaccatta acagagaata ctaaaaactt
    52981 agttaaattt ttgtagctat ttataacaca atattctgaa ccttttttac ccataaggta
    53041 gttatattca aaattgtata tgggaagtgt atctagttta agacctacat tttttccctt
    53101 accatagaat acgattttag aatctccttc aaaacatata gatatactac cattgttgaa
    53161 tttaattaat atactaaaat agttagcctc aaattcataa tctacaggca tgttatttac
    53221 tgtaggtata atccttagta catccatatt tttctttgat tttcctctta atgattttaa
    53281 tgtaaatcca agttcatctg aataagaaat agacatataa gatccaaagt aactaaacgg
    53341 aatttctttt atattcattt ggtatagtcc tttcatgatt tattttatag atttactctg
    53401 cttgtttata caagtaagta tatatttaac ttaccaaata ataaaataat tatcaagagt
    53461 taatttatca aaaaaataaa taagaaaatg ttttcaaaaa actattgact aaaataatat
    53521 aaaatattac aataaagttg ttcaaacaag ttacttgttt aaacaactta ttacttataa
    53581 taaaaaagag aaaggataag tagatatgag gaagaataag atactaaatt caaaagaaag
    53641 tatttccaaa attattgagt atgttggggg aaaagaaaat atacaaagtg caacacactg
    53701 tgtaacaaga ttacgtttaa tattaaaaga tactagcaaa gcaaatatta gtaaactaga
    53761 agacattgat atagtaaagg ggtgtttcaa tgcaggcgga cagtttcaaa tagtaatagg
    53821 aactggagta gataaagtat ataaagattt aatagaccaa acaggagcag aagaagtatc
    53881 caaagaagaa attaaacaac aagtcaatga aaagtcaaat aaatttcaaa agtttataag
    53941 aatccttgca gatatattca ttcctatatt accagctata gttgcatcag ggttacttat
    54001 gggtgttaat aatatgatag ctaatccagg gattttttat gagaagtctg ttatagaagt
    54061 aaacacagat atagctggga tttcaggtat tataaattta atagcaaaca catcatttac
    54121 atttttacca gcattaatag gttggtcagc tgcgaagaaa tttggaggaa atcctttatt
    54181 aggtgtagtt ttaggtcttg tattagttca cccagctttg atgtctgcat atgattttgc
    54241 agcaaatcca tcagcagcac caacatggga tatatttggt atgaaggttg ctcaagttgg
    54301 ataccaaggt caagtaatac cagttttaat agctacatat attttagcta aacttgaatt
    54361 attcttaaag aaaactgttc cagatgcagt tcaactagta ttagtttcac caatagcttt
    54421 attaattaca ggtatgttaa cttttgtagt tataggacct ataactatga caggtgcaaa
    54481 tatgataact aatggagtag tatatttatt tgaagtagca ccagttttcg caggagcact
    54541 atattcattt atttcaccac cattagtaat aacaggaatg catcatttgt ttttaggagt
    54601 aaacttacaa atggcaggaa ctttaggata tgtaacattg tggccaatat cagagactgt
    54661 tactttagct caaggtgctg ccacattggc aatgtttttc atacttaaaa ataataagaa
    54721 tttaaggggt gtatctatta catctactat atcagcatgg ctaggaataa cagaaccagc
    54781 tatatatggt ataaacttaa gatttagata tccatttata gcagttatgg tagcaagtgc
    54841 aataggagga gcatttatat catatcacaa tgttaaagct acagctgttg gggttggcgg
    54901 agtattttca ttcctttcag tgatgcctca atattgggga gtatatttta cgggaatggc
    54961 aataacattt gtaataacaa taggattaac atacttattc tataagagtg gtaaatttaa
    55021 aacaaatgac aatatataaa tataattccc ttggttttat atcaagggat gttaatatgc
    55081 attggataga taggagacaa tatgttagat tttaaaaaga gtatagtgta tcaaatatat
    55141 ttaaagtcgt ttaatgatac aacaaataat ggtttaggag atttaaatgg aataacggaa
    55201 aaattagatt atttagaatt tttaggtgtt gattatgtgt ggattactcc attttttgta
    55261 tcaccacaaa atgataatgg atatgatgta gctgattatt ataatataga ccctatatat
    55321 ggaactatgg atgatttaga aaatttaata aaagaagcta aaaaaagaaa tataggtatc
    55381 atgcttgaca tggtgtttaa tcatacgtct atagagaatg agtggttcaa aaaagcactt
    55441 aaaggagatg aatactacaa agatttttat atatttaaaa atcctaaaga agataaaagt
    55501 gctccaacaa attgggaatc aaaatttgga ggcaatgctt gggaatttgt agaagaattt
    55561 aatcaatatt atttacattt atttgataaa actcaagcag atttaaactg ggaaaatcct
    55621 aatgtaagaa aagaattaaa aaatatatta aagttttgga tagataaagg tataaaagga
    55681 tttagatttg atgtcgtaaa tttaatatct aaaccagaga agtatgaaga tgatttacaa
    55741 gatgatggta gacgttttta cacagatggt aaaaaagtac atcaatactt aaaagagatg
    55801 tgtgaagaaa gtggaatatt agaaaatgat atgttaactg taggagaaat gtcttctacg
    55861 actatagaaa actgtgtaaa atattcaaat ccagatgaga aagaattgtc tatgtgtttt
    55921 aattttcatc atttaaaagt tgattataaa aatcaaaata aatggatgtt gcaagattgt
    55981 gattttatta agttaaaaaa aatacttaat gagtggcaaa taggaatgca aaaaggtaat
    56041 ggatggaatg ctttattttg gtgtaaccat gaccaaccac gcataatatc tagatttgga
    56101 gatgataaaa agtattataa agaatcggct aaaatgttag ctactatgat ttatctctta
    56161 agaggaacac catatatata tcaaggtgaa gaaataggta tgactaatgc atgttttact
    56221 aatataaaac aatatgtaga tgttgagagt ttaaattatt ttgatatatt aaaatctaaa
    56281 ggaatgaaag atggtgagat atacaatata ttacaagaaa ggtctcgtga taattcaaga
    56341 acacctatgc aatggaataa tagtaagaat gcaggtttta gcgaatcaaa atcatggata
    56401 gaggttataa ataattatag aaatataaat gtagaaaata gtttaaaaga taaggattct
    56461 attttttatt attataagaa gttaatagca ttaagaaaaa agtatgatgt agtagcctat
    56521 ggggatttta gaccattatt agaagaacat gattctatat ttgcatataa gagatgttac
    56581 gaaaaagaat ctttagtagt gttaaataac ttttatgata aagaaattga tgttgtttta
    56641 gagaatgaag agcttgaaaa atacaagtgt ataatatcta attatgggga cagaaaaatt
    56701 gtttcaacaa ttactttaag accgtatgag tgtatagctt tatttaaaaa tgaaaaataa
    56761 tctaaaatta aaatcatgaa aaatttatgt atacttttta gataaatttt atttttgaag
    56821 tcataattta atatttatta gtaaggctgt gattgtaaaa atcatagcct tattttaata
    56881 ttttttccac atatacaaat tattgtttat attaaggaaa tttaatttat attaaatgta
    56941 tagacatttt ctttgtttgt atataaaatt atcttattat ataaaaaatt taattaatga
    57001 ggagtgaatt aaatgaaaga gccaatttat aaagttatag aaaatcatgt tagagagtta
    57061 ataaattcag acagcttgaa agaaggggat ttaatccctt ctgaaaaaca gttgagtgaa
    57121 gagtttaacg taacgaggat gactgtaagg tctgcactta ataacctagt taaagaagga
    57181 tatataacta gacaaagagg agtaggtagt atagttcttg caaataatat atacgataat
    57241 atatcatctg taagtgggtt tactaaagag atggaaagca aagggtataa agtatctaat
    57301 atattggtat ctcttgaaat agttcaagca gatgaagaac tatcaggtaa attaaatata
    57361 agtttagaag aaaatgtttg ggaaatcaaa agggtaaggc tagcaaatga tgcaagagtt
    57421 tcatatatga ttacttatat gccagttaag ttatttccaa atttaaataa gactcattgt
    57481 gaaaattctc tttataattt tgttgaagaa gtgtgtgcat ataagatagc catgtcagag
    57541 agagaagtac aggcagtaat atcgaataaa gagtgtatgg ataatctaaa actagaagaa
    57601 ccagagccac ttttatatat aagccaaatc tgtaagttac aaaatagtga gatttttgaa
    57661 tactcacaca catatcacta tggatataca ttaactttga atgctgttgt cgaataaaat
    57721 ttaaataaaa tgaaaaagtt ttcttgaaat gtatatacat ttggtatata ataatatcaa
    57781 gaattaaatg tatatacatt taaaaataag atatatacat taagaaaaga ttaatagaaa
    57841 agcatacttt aaatgtatag tttaaaaaac ttatgactta aaaagtattt aatgaattta
    57901 atttggaggg gaaaaaatga taaaaataat gttagcttgt tcagcaggaa tgtcaactag
    57961 tttattggtt acaaaaatgg aggatgcagc aaaggaaaac ggtatagaag caaaaatatg
    58021 ggcaatatca gaagtgaact taaaaaatga aatagaaaat tgtgatgtct tacttttagg
    58081 acctcaagtt agatatgtat tgggaaaagc aactgaaatg gcaaaaccat ataatattcc
    58141 agtagaagtt ataaatatga tggattatgg aagatgtaat ggaaaagctg tgttagacag
    58201 agctgtagag ttgaatagtt caaaataaag tcgtactgga ggatgtaaaa tggaaaaatt
    58261 catgtcattt atggataaat atatagtgcc tgtagctgct aaaataggag ctcaaaggca
    58321 cttggtagca gtaagggatg catttatagt aatgatacct ataactatgg taggtgcatt
    58381 aggaacattg ataaataact taccattaga agcatacaaa aatttgatgg caagtatatt
    58441 tggagaaaac tggactacgt ttggaggaga cctttggtgg ggagcaatag gtacaatggc
    58501 agtcttctta gtaataggtg ttgcctattt tctagcaaaa tcttatgaaa gtgatggatt
    58561 acaagctggg cttatagcat tatctatctt ttttataatg gccccacaaa ttggtaaaat
    58621 agttcctgag ggaggaacta cagtagttga aggatgggga atgatacaac aaacttactt
    58681 aggtacagca gccttattct cttctatatt aataggattg ctatcaactg agatatttgt
    58741 aagattatct aaagtaaaga aattaactat aaaaatgcca gatggagttc caccagcagt
    58801 ttcaagatca tttgcaaagt taataccagg aatgttaact ataatgatat ttactgtaat
    58861 aggaatattt ataaaaatgc tttcaaatgg aagtttctta acagatattt taaatactta
    58921 tttaggagca ccactatcaa atgtagctga tagcctaggt tctacaatgc ttattgcatt
    58981 tataattcat atactttgga ctgttggact tcatggtgca aatatagcac ttccttttac
    59041 agaaactata cttatgaaat taggtggaga aaatgctgcc ttggctcaag caggagcaac
    59101 agaaggatac catgtacttg caggtgcatt ctttgatgca tttgtatatt taggcggttc
    59161 tggaatggta ttgggattaa ttgtagcttt attaatagct ggaagaagac gtaaagaaat
    59221 gatagtatta ggtggaccac ctgcaatatt taacatagga gaaccattga tattcggact
    59281 tccaatagtt ttaaatccta tatttatgat tccatttgta cttgcaccag taatatgtag
    59341 tgctgtatca tacttagcaa tagattttgg gttagtagca ccagtaattt tacctaaaat
    59401 accttgggta acacctccta tattaggtgg agcaatggct acaggtgact ggactggtgg
    59461 agctttagca ctatttaatt taatattatc aatattaata tatattccat ttgttatagc
    59521 gtctgaaaaa atggaggcta ataaattgaa aattaacaac taggtagttt tatttcggta
    59581 aataaatgta tagggttttg tatagtgtat taaattatag gtaaggatgg tataatgaga
    59641 tgaaaaaggg actaaaaata gttacaatcg gaggaggttc aagttatact cctgagcttg
    59701 tagagggttt tataaataga tatgaagagc tacctgtaaa ggaattatgg cttgtagata
    59761 tagaagatgg aaaagaaaaa ctagagatag ttgggaatct agctaaaagg atggtaaaaa
    59821 aagctggaat agatatgcaa ataaatctta ccttagatag aagagaagca ctaaaagatg
    59881 cagattttgt gacaacacaa cttagagtag gtcttttaga tgcgagaata aaagatgaaa
    59941 ctattccttt atctcatgga gttatgggac aggaaactaa tggtgctgga ggtttattta
    60001 aagcccttcg cacaatacca gttatatttg atataataaa agatgtggaa gaattgtgtc
    60061 caaatgcatg gatggtgaat tttacaaatc caacaggaat aataacagag gctgtattca
    60121 aatatactaa ttttagaaga tatataggtc tatgcaatgt gccaattcat ttaaaaaatg
    60181 atgttgccaa actatttaat gtagaaggtg acagaataag tatggatttt gcaggtctta
    60241 atcatatggt ttatggtcta aatgtatctt tagatggaga agatgttaca aaagaagcaa
    60301 ttgataagtt tgtcaaagca gatataagta tgcaaaatat aaaggcaatt gattttaatg
    60361 ctgaatttgt aaaatctttg ggtgcgatac cttgtccata tcatagatac tactataaaa
    60421 ctaaagaaat gcttgaagat gagttaagag agtttaaaga gggaaaagct agaggtcaag
    60481 tagttaaaga attggaagaa caattatttg aattatataa ggatgaaaaa ttagatgtaa
    60541 aaccacctca acttgaaaaa aggggaggag cttactatag tgatgctgct tgtaatttaa
    60601 taagttctat atacaatgat aaaaaagata tacaagtagt taatacatta aataatggtt
    60661 ctataagaga ttttaaagat gaacaagcag tagaagtaag cagtgtaata acaaaaaatg
    60721 gtccaaaacc aataagtata gggtatttac cagattctgt gcatggatta gtaagccaaa
    60781 tcaaatcatt tgaagtattg gcagctaaag cagccgtata tggtgattat gaatctgctc
    60841 ttttggcact ttgtataaac ccattgattc catctgatga cttagcaaaa actatattag
    60901 acgaaatgtt ggaagctcat aaagattatc ttcctaaatt taatagatag ttgtctatag
    60961 aaagtaggtt atggtatgac taaaattata ataaatgcag atgactttgg atattgtgaa
    61021 gctgtgaact atgggatagt gtcagttcat aataatggta tagttaggtc tacttctatg
    61081 atggcaaata tgcctggggt agaacatgga gtagggttac ttaaagaaaa taagtcttta
    61141 aattgtggag ttcatatgac tttaagttgt ggtagaccat tattgtctaa cttaaaaact
    61201 attgttgata aagacggttt ttttattagg aggattacag acgaaattat tgaaaaaatg
    61261 gattatgatg agatttatag agaactttgt gctcaaatag atagagtaaa agggcttgga
    61321 atagatatat ctcatttaga cagtcaccat catatccata ctttaataag tttaaagcca
    61381 gtaatagaga agattgtgaa caagtataat cttccaataa gaggtggatt tgaatataat
    61441 ttagagtatt ctaaaatagt tcctttaata gatagttttt ataaggaaaa tgtatgtgaa
    61501 gaatatttta taaaaaatat agacgaaata atgaagtatg atgtagtgga tattatgtct
    61561 cacccagctt ttttagatga ttatatttta aattctacat cttatgctat tgatagaacg
    61621 aaagagcaca agatacttac atctaaaaaa gtcaaagaat ttttagataa gaatgattta
    61681 gtcatatcaa gttatagaga tatttaggag gacgaataaa tggatgagaa gatgatagag
    61741 atatcttttg gaataattgg atttgcaggt gatgcgaaag ggctagcatt tgaagcaata
    61801 aaagaagcaa agagcgggaa tatagataaa gctagagaat gtattgaaaa gtcgaaagaa
    61861 tcaattcata aagctcataa attccaaaca gaattgatac aaaatgaagc taatggtaat
    61921 aaaactgaaa taagtgtaat acttattcat gcacaagacc atttaatgac agctatgaat
    61981 tttcaacaat tagcagaaga atttatagat gtatatgaaa gattagaaag taagtaattt
    62041 aaaattatta ttaaaaaaga gtgcctttaa taattataaa aattattttt ataattatta
    62101 aaggcactct tttttattta agtagagttt agtaatatag tattatgtta ttgttacttt
    62161 aaaatttcga tttttcataa ttgtaaggta tactagtgcc aacaactaca tcttcaattt
    62221 tttcaataat aagccaatca tccatatttc cttgatggtc aaaatcaaga ggtgttattt
    62281 cttcaacatt ttcaaattca actaagcata aacatttttt atgccaacga actttttgct
    62341 tttcggataa atttaatttt ggttgatttt catttaaaat tttagaaatc tcgtcttctg
    62401 ttaacttaac atagttttct acttttttaa caatggcttt tgcagatatt tttgcagttc
    62461 ctttttccat aaaataaagt aactcatcct taaatactct actgtgtggt atttttctac
    62521 cagcagcacc tctaatcacc attgtttttg ttccagctaa gattttatct aaaacctttt
    62581 ctcctttttt tcctgcatta tcacaatata ctaaatgtac catttttcga atcctcctta
    62641 ctaacttcct tatctataag ttgcatttta tcattgttca actaaaaagt cattctattt
    62701 atatctaatt ttttaaaaaa tactatttaa tatgagaaaa ccttatctga gctatatata
    62761 attaagatta attaggaatt tttatctttt tacaaaataa tattgactaa aaatagaaaa
    62821 atgctataat aaatatatgc ttatgataat caatatcata taagtataat aaacagctag
    62881 gagaaataat catgaaaaaa ataaaatcac ttgcaatatt tatatctatt ataactacgt
    62941 tagtacttgt tacagcatgt tcagataaga atgcagagga taaagataaa tcagagacaa
    63001 gagttgtaca atctgttaaa ggagaggtta agataccatc aaatcctaaa aaaatagttg
    63061 acatatcagg aagtagtgaa gaactactat tagcaggtta taaacctatt gcaacagcaa
    63121 atgtagactc atatgaaact gataaattac caagttacat aagagaagaa cttaaagatg
    63181 taaagatagt tgggcactca atgatggata ctatggatat ggaagctata ttagaagtaa
    63241 atcctgattt aataataatg agtcaaagac aagaaaaaat atatgaccaa ttaaaagaaa
    63301 tagctcctgt agttatgatg aaagattatg caaatgactg gagaagtaag ttaactgatg
    63361 tttcaaggtt atttgacaaa gaagaagaaa ctaaaagttg gctacagaag tatgatgaaa
    63421 aagctacaaa gttaggtaaa gaagttatag aaaagaatgg agaaaaaact tatttaccag
    63481 tgttggcaag ttcagggcaa tttatggtat ttagtgatgg tggaataggt actctaatca
    63541 atgatgacat gaaattagct agacctaaga atatgccaaa acaggatgga ataacactac
    63601 caatgataag tatggaaggt ttaacagata tagatgcaga ccatatagtt gtaattgcta
    63661 cagaatcaga taaaaaagat ttagaaaata gtgctatctg ggctcaaata agagcagtaa
    63721 aagatggtaa tgtaacaata cttgattctg ctccattttt cagtcaatca tacaatccaa
    63781 tgggaaaaga gttattatta gaatcagtta aaaatgaatt aacaaagtaa ttcttaactt
    63841 agtaggtgtg agataatgat attcattttt cacacctatt tttaataaag cttaaaataa
    63901 aaaatattta atagatgata ttatactgac tattaaaagg gaaaaggagg taggataatt
    63961 gactaaggta agcaaaaaaa atataactta tttattgata ttagtaagta tagttgtact
    64021 tgtatttgga attttattat ccataactat tggggcaaaa gatatgaatc tatcaactgt
    64081 tatagatagc ttgataaaaa tggaagatgg aataaatatt agaatagtaa gggatgttag
    64141 acttccaaga gcaatagcag ctacacttgt tggaggtttt ttagctgttt ccggggcaat
    64201 aatgcaaggg ataacaagaa atcctatagc tgaaccttct gttataggaa ttacacaagg
    64261 agctacattt acaatttcaa tatcactggt acttcaaaaa aaacttcctc aacttataca
    64321 tggaagtttt agtgtaatga tgtttgcatt tattggagca agtataagtg gattatttat
    64381 atattttata agttctaaat caagaggaag agttaataat gtaaaattag cacttgcagg
    64441 agtagctctt ggtacattgt taatttcctt ggcatcagca atatctatgt attttaacct
    64501 atctcaacag ttgagtttct ggatatcagg agggcttgta ggagtgaatt gggaaggtat
    64561 aaaattgcta tttatagctg gaggtattgg atttatttta gctattatta tggctccaag
    64621 aataactata ttaagtcttg gagaagaagt ggcaatagga ttagggcaaa aaactaacat
    64681 agttagattt gtatgtatag tacttgtgat actaatgact ggagcatctg tttcagtgtc
    64741 tggcaatatt atttttatag gtcttatagt tcctcaaata gctaaaggaa tagtaggttc
    64801 agattataag tatataatac cgagttcttt agtattagga gctgtattgc ttgtatatac
    64861 agatatatta agtaggatga taaatcctcc atatgagaca ccagtaggtt ctataacagc
    64921 acttatagga gtgccaatat ttatatacct tgttagaaag ggggagaaat aggtactatg
    64981 aaaaatatta taaaaataaa ttctaaaaca ttgcttgtta tattaatttt agtggtactt
    65041 atttttatca ctcttctagt gagtttaaat atgggctcat tttctataga accaatggat
    65101 gttataaaga cttttttagg tcagggtcag cgtaatcatg aaattgcgat atttaaattg
    65161 agattaccaa gaatagtaat agcacttttg gttggttctg cattatcaac agcaggtgta
    65221 atacttcaag gagttacaaa gaatgattta gctgattctg gtattttggg aataaattca
    65281 ggtgctgcat tgtttgtagt agtttatata taccttatga atggtaatgt atatgaaggt
    65341 atgagtaatc tgactgtatt tacaatgcct attgttgcac ttgtaggagc tttatttgga
    65401 gcatttctta tatattctct tgcatggaaa ggtggtataa attcgtcaag attactactt
    65461 attggtatag gtgtaaatat agcttttact tcaattttga ctatatttca attgaaattt
    65521 acaacacaag aatttaatag ggttatggcg tggacatcag gaagtgtttg gggaactagt
    65581 tggaaatatg taatagctgt acttccattt atagtgattt ttatggggat aactatttat
    65641 aaatccaaat atatagatgt attaaacttg ggtgatgaag tatctataag tcttggtgta
    65701 gatgtagaaa aacaaagaag aagacttata atatatgcag taattttatc tggtgtatcg
    65761 acagcagtag caggtagtat ttcattttta ggtttaatag ctccacatat aggtagaaaa
    65821 ataataggtc caaagcataa aagattaata ccagtatcag cattgatagg aacattattg
    65881 cttttaatag cagatactat atcaagaaat cttctagcac ctatagaaat acccgtaggt
    65941 atagtagttt ctataatagg agtgccgtac tttatttatt taatgttggt aaatgattag
    66001 gaaagggtgt tgttatgaac tgtataaata ctaagaatct aaatgtatca tatggaaata
    66061 cagatattat aaaaaatcta aatttaaata taccaaaagg aaaaataact actatcatag
    66121 gggctaatgg ttgtggaaaa tcgacaatac ttaaaactat aggtaggatt attagcccta
    66181 agagtggaaa aatatatata aatggagagg atatctacaa aaagaagtct aaagaaatag
    66241 caaaggatat ggctgtactt ccacaaagtc cacaagctcc tagtggtctt actgtttcag
    66301 aattaatttc ttatggaagg tttcctcata aaagtggatt tggtaattct agtaaagaag
    66361 acagagagat tgtaaattgg agtttagaag ttactggaat tgcagaattt aaggatagaa
    66421 atatggatga tttatcagga gggcaaaggc aaagggcatg gatagctatg gctttggcac
    66481 aagagactga tatattatta ttagatgagc ctacaacata tttagatttg gcacatcaat
    66541 tagaaatact aaaattattg gatgaattaa ataaaaaaca aggaagaact attgtcatgg
    66601 taatacatga acttaataat gcagcaagat ttgctgacca catgataggt attaagaaag
    66661 gaaaaatagt ttgtgaaggt gatgcatatg atgttatgac gaaagataat ctaaaagaat
    66721 tatttaatat tgatgcagaa atagcaacag acccaagaac aaataaacca atttgtataa
    66781 cttatgacat agtgtaaatg gtgacaacat ggagaatcaa gaggcattaa gacatgaaaa
    66841 aatatggatt ttattattta aatattcgat acctgcaata atagcaatga tggtaacttc
    66901 actttataat gtagtagata gagcttttat tggttctatg gagggcattg gttctatagc
    66961 tattgcaggt cttggtgtaa ctatgccagt atttactcta ataatagctt ttggaatgtt
    67021 ggtatcagta ggtgctagca caagattatc tataaagcta ggtgaaagaa atagagaaga
    67081 agcagagaag atattaggta atgctttgac attatctata attatctctt taattataac
    67141 tatattagga ttaatttttt tagaagatat gctttttatt ttgggtgcta gtaaagactc
    67201 tatttcttat gcaaaagact atatgagtgt catattagtt ggaagtatat tcaatttagt
    67261 tgcattttct cttaataatg caattagggc agaaggtaat ccaaaattag cagcaaggac
    67321 aatgattgta gggtgtgtat taaacttgat attggaccct atattcatat ttgtgtttaa
    67381 tttaggtata aaaggagcag ctatagcaac agtactctgt caaatagtag tattcttttg
    67441 ggtaacacat tattttatga gaggtaagtc caatttaaaa ctaaaaatat gtaatttaaa
    67501 attgaataaa gatatagtaa agaaggtatt tgcaataggt atgacacctt ttgctatgga
    67561 ggtagctata agtataactc acatgtttac aaataattcg ctaaaggttt atggtgggga
    67621 tttagctatt ggggctatga cagcacttac atctatttta ttgatgttta tgatgcctgt
    67681 atttggattg aatcaaggta tgcaaactat aatatcatac aattatggtg caaaaaaata
    67741 tgagcgtgca aagaaaactt tgatattatc tatcatagtg tcagttgtca tattatcgtt
    67801 tgggttttta gtagtacaag ttttcccaga agtatttgta ggtatattta ataaagaccc
    67861 agatttaatg aatatagcag taagaggtat aaatataaat ctaattacat tgcctataat
    67921 gggaatttct attgtagggc ctgtgtattt tcaatgtata agtaaagtta aacactcaat
    67981 gtttttaact cttttaagac aatttatttt atttatacca ttaatcattg tacttccaat
    68041 tcaatttaat ttagatggag tttggttggc acagccaatt gcagatttta ttgctatgat
    68101 aattgtatta ttattcttaa aaagggagtt taaaacaagt caagtataag aaatctagaa
    68161 attaaaatga aattagaaga taaaaagact tgtctttata aagtgattaa aattatttta
    68221 taaagacaag tcttttattt tatttaacga aactatccat actggtttct tctagtgttt
    68281 ttccaaaagt cattttctca aatatttctt gaggcagagg ttttgaaaaa acatatcctt
    68341 gtatcatatc acaattaatt tttttaagat aatccatttg caagattgtt tcaacgcctt
    68401 ctgagatact attcatattt aatttttttg tcagattaac tatagattct attatatgat
    68461 tttcagcaat attatctgca ttaggtgaac taaaaaaagc acggtcaagt tttaatgtat
    68521 caacttttat ttcttttaac atattgagtg aagaatatcc acttccaaag tcatctagtg
    68581 aacattgaaa accttcttca tggatttggt ctattatatt aattagtttt tgtagatttt
    68641 caaatacaag tgtttcagtt aattcaattt ctaagagctt agctggaata tcatatttat
    68701 tacgaatcat tctgtatttt tctaaaaaat ctatattatt taaattagca cgtgaaaaat
    68761 taactgaaac aggtattgga gtttctccat tatctatcca tttccttatc atactacata
    68821 ctttttcaaa aacatagtgg tctagttttg taataaaacc atttttttca aagaaaggaa
    68881 taaactcatc tggtggtata agacctttat ttggctcttg ccatcttact aatgcttcag
    68941 cacctacaat agtattatct ttaagactga ttttaggctg aagatatact ataaattcgt
    69001 tattatttaa tgcttcctcc attctatttt ccatttcttt ttctttaatc atttttaagc
    69061 gttctaaatc actataaaaa acacatgtat aaagtggatg atttccaata tttcctttat
    69121 tgttttttcg tgcaacattt gcacggtctt gaattgaaac taaaggtaga tttgggtcat
    69181 caattttata tacacctata gatagagcta taaaatactt gcattcttca tttttattga
    69241 aactgttaat atcattagta atgttttcta tgtaactaag aatttctttt tgaggaatag
    69301 ttttaataag tacattaaac tggtcattag aaactcgaca aagtaattct tctggtttta
    69361 gataattact aataacatca tgtatgtact ttaatgtttt atcacctgct tcactaccaa
    69421 aagaatcatt aataagtttg aacttctgaa tgtttataga taccaatgaa tagctttctg
    69481 atgaagatga ttgtataatt tttttagctt ccatatcaaa gcgtgctttg ctgtaacctt
    69541 tagttacaga gtctacatat gcgattttta taagttgtgc cctgtttttt tgatttataa
    69601 acataatcaa tataataagc aataaaaata gtataacaat taaaatattg attaagattg
    69661 ctttttttag aagtaaatca aactgtgcac ttgctgtttt tgttggaaca atcgaaagga
    69721 gatataagtt ttcttcttgt aatggaatat agtgcattgc tttagaaata ccatcatcta
    69781 atgtataata catagaacca gttttgttct tttttaagtt tgttttcatt tcatcaagct
    69841 tatttttatc aacctgtttt cctttatgtt caatcatgtc aaaaaaattt accacattct
    69901 ttggagcatt tttgtttgcc gaacttacaa taaaatttcc attttggtcg ataatttggg
    69961 aaaatccttc accattaaag ctttctacac ttaataaatt tctcatagta tcaatagtat
    70021 tccaagcagc aacagctcca acaattttat catctttgta tagtggtaca gaatataaaa
    70081 ttccatatgt accaaatggt gatttaacag gcgtaattgt ttctttacct tgaagggctt
    70141 ttatataaaa gtcttgtcct ttaacattac ctgtatcttt aagggcactt atgaaatttc
    70201 cattagaatt gataatacct aaccaatcaa attcaaataa ttttaatttt ttggataagt
    70261 atttatttac ttctttgtca ctatcattaa cctcaaaata agtatctccc acagaatata
    70321 gtgtcttaaa tatactttta catttcacat caattgtggt agaaatgctt tgtgaaattt
    70381 ctataagata tcgttctgtt tcattatgtg ttactttatt tagattcata ataaaagtga
    70441 cacatgttaa ggacacaatt ataaggagtg tcaatagtaa agtagctatt atttttttat
    70501 tttttgtgtt ctcaaagttc attcttttgt ttcatcctcc atcctctatt tgtttacaaa
    70561 taagaattct ctcagacata ttataccaaa tatcttttca tacaaagaca ttaaatttta
    70621 tttatttgac tttatttaat ctttttaggg agtagataag atatatttat agtttaaatc
    70681 gtattttatg atgttttaag taaaagagga taaaaaggaa ctgttctcaa aataactttg
    70741 atacagttcc tttttagtat tgtaaatttt tataagatta gaaagggttt ttagtttata
    70801 catctacata agtattatct ttataaaaaa tattttattg tctattgcaa tctaaaatgt
    70861 agatgaaatt ctgtaaatac caaattctct atgaccaaga ctttcagctt tggtatgctt
    70921 accattacag acttccacta tttcattaaa tacttcttga cctatatctg ctattttagc
    70981 gccatctgtt ataattctac ctgcatttat atcgatattg tctggcattt tgttgtatgt
    71041 atcactattt ccagtgattt taataactgg tgaaatagga cttcctgttg gagtacctct
    71101 accagttgaa aagattacaa tttgtgcacc acctgctacc ataccagtga tagaatctat
    71161 atcctgacct ggagtatcca taaaatataa acctttttta tcagtaggta cagtatcagc
    71221 atactcaaga gcacctttga atggcttgtt gcctgcttta tacatacatc caagtgattt
    71281 ttcttcaatt gttgaaagtc cgccagcttt attgcctgga gtaggttgac cactgcgtaa
    71341 atcttctccc atagtaattg ctcttttttc tacatcactt acaaatttaa gaaacttatc
    71401 cttcattttt ccatcttcaa aacgagtagc caacaaatgt tctgcaccaa ttacttctgt
    71461 agtttcactt agtatgctac ttccaccttc atctataaga agatttgatg caacacctat
    71521 tgaagggttt gaagctattc cacttgttgg gtcagaacca ccacattcaa gtgctaatat
    71581 aatttcactc atatcaaatt caactcttac ttcttttgat acttcacgag ccattttacc
    71641 tactatttca gctcccttag aaactgtttt taatgtccca ccacattctt gtataacaac
    71701 ataatctacc ttttttcctg ttgtagctat ttcagatgat aagtcttttg cttgaatacc
    71761 atcacatcca agaccaacta ctaaaactgc ggcgacatta ggattttttc caaatcctat
    71821 taatgtcttt gctgtaagct ctatatcaga accaatttga caacatccat gttgatgagg
    71881 gatggctatt gctccaggta caagtgatgc tattcttgta gctgtctcac ttgaacatac
    71941 agatgttgga ataactaaaa catgattacg aataccaaat tgtccatttt ctcttaaata
    72001 acccattaat ttcattttaa cttcctccta ttttttatct ccacgaccac gtgtactttc
    72061 aatgttgtgt acatgaacat agtcaccagc ttttatgtct actgttgcaa ttcctataac
    72121 ttcaccgtat ttgtaaacaa cgtctccttt ttttacattt tctgtagcca tcttatgaaa
    72181 gacaggtata ttatcattta ctatgtaaga ctttccatca attgaaattt tttcaccttt
    72241 aactaaaggt ctgacacatg taactagatt atctttttca tttgcatgaa taattatatt
    72301 cattaatttt acctccttaa gtattaattt acttcaatat ttttatttgt tttaaaaata
    72361 gcttttttct ttttttctct tttatctaga taggaaacta acattggaca acatattgct
    72421 gtaacaataa tagcagcagc aacctgtact gttgaaacag ctgcaattgt agctagagta
    72481 gggtcgatag ctgctaaagc ttcaggagta ccagctgcat ttccggctgt tgtacctgta
    72541 gctgcaccta ctgctgggtg ttctgcacgt ataagtttca ttacaaaata cccacccata
    72601 cctgttatta ttgtaactcc taaaccaagt ataactccag gtaatccagc agttataatt
    72661 tgattaaagt ttaatgctgc ccctaaagga aaagcgaaga aaggaattaa taatgttgta
    72721 ccaggtgcaa gaaattctcg tatatcttca tcgagattac caagtataaa tccaagtagt
    72781 attggtataa gtgctgcaaa tagtgctact ataggtacat ttgcaagtcc agttactcca
    72841 aaagcaagca ttgtgaaaaa aggaccatca tttagagata agatagatat tgctccaaca
    72901 tctgtagagt ctccatattc acctgcaaga gcagcaaata gaccaccatt tgaattagtc
    72961 atagtagcaa ctatggcaag aggtgtaata ccaattatac ctttaggtcc aataaattta
    73021 ctaacaataa tacctaatac tgcaccaatt ataaattttg ttgctgtaag tactatacct
    73081 ttagtcaatg gattaccagc ttgtctgatg ttgatttgag ctccattaca tagacaaaat
    73141 agagcaagta tgctcattgc accagtttta aataaggatg ttgtgaatcc tccaatttca
    73201 agagcttgtg gatatactgt attaaagagt gctcccaata acaggggaca gaccataagt
    73261 cctcctggta tctttttcac tgattttaag atgtgcatct agtatcactc cctttcattt
    73321 ttatataggt atttaaaagc aattactatg ccaatatata gagtttatat ttttagttat
    73381 tatgattatt tttgataatc gattgaaata ctgtagatta cttataatta aaagtatgat
    73441 tgaggagaat tttacattaa aaagattctt tttatcatat aattgtattt tttattaaaa
    73501 ataaataaac gtcttatgta ttaaaattaa acataaaacg ttttgatata ttaagtatgt
    73561 attattttta aacatcattt tctatttctt ttaacttacg ccatagagta gagcgagtta
    73621 tctttagaat ttcacaggtt tttgtagtat ttctattatt ttcgttataa attttataga
    73681 tatatttttt ttctaaagtt tttaaatcta aattattata ttcaaaatca gtattatttt
    73741 tatcaacaat attatttgta tattcattac tatccttttc taataaaatc gagtcaaatg
    73801 attccacaat aacagccctt tccattatac cttccaattc tcttatatta ccttcatatt
    73861 cccgttgaag aagtgctttt ttaatatttg gagtgataat taaaggcgtc ttattgtatt
    73921 tgtttgaata tttatgtata aaaaattctg ataattttat aatatcttct tttctttctt
    73981 ttaatgaagg aagatataat gttagtatat ttattctaaa ataaaggtct tgcctaaata
    74041 aaccgttgcg catcagattt tttaagtctt tatttgaagc acagataatt tttacatcta
    74101 taggaataac tttattactg ccaattctca taacttgttt ttcctgcaat accctcaata
    74161 atcgaccttg aagttcaaga gggatttcac ttatttcatc taagaagatt gtaccttcat
    74221 gtgctagttc aaatacgcct gctttgcctt tttttctagc acctgtaaat gaaccttctt
    74281 cataaccaaa aaactcactc tctattaaag atggaggtat ggcagcacaa tttacagcaa
    74341 caaaaggtgc atttttacgc ttactatagt tatgtatgct ttgacaaaat agttccttac
    74401 caacgccaga ttcaccagta ataatcattg gtgtatcata acaagcatat ttttttgcga
    74461 gttctataca atcttttgtc ttttcactag catgtattat atcatcaaaa gtgtatttag
    74521 cagagagacc tttatttaat aagctccttc ttattgtatg ttcaaaagag cttatctcct
    74581 tgctgtcttg aaatgttgaa acagctcctt gaatttgacc atcaacaatg attggaactc
    74641 tatttgtaat aatttttgta cttcctatat tttgtatttc accaatttga ggttccatag
    74701 tttcaaccac ttgtggaaga actgtgtttg gaataacgtc tataacatta tgataaagtg
    74761 cattttcttt ttttatacca aatatttttt cggcactctt attaaataat gttattttaa
    74821 aatttttatc aactgttatt attccatcat gtacaaaatc tgtcatagct tctatctgta
    74881 atcttctttt ttgttcatcc ttggttgctt cgagtatttg ctctgcttgt tgaatggctc
    74941 taagtatgtt ttcttctcta gattcgatta atattccctt gcaattaagt ctcttaacaa
    75001 tatgtgcaac agtggtgtct cctatatacg tgtttatacc tttctttctg tacttttcta
    75061 tagcatcata aatttcctct tctttttcaa tttcaatcat agttattttc ttattcaata
    75121 tttcttctat aatatcgaaa ccgtagatga cattattaaa tccaattatt ccaaatggtt
    75181 catctgaatt ttttacctct ttgtagcttt ttataatatc ataagcgtct actttaattt
    75241 caactatagg tatattgtaa gtggctttta acatattgta agttccacct cggcttatga
    75301 taattctagt accatgatta atactttttt cagcaagagg tataccttct tttagattgc
    75361 caaacataac atcaatattt gtatatttgt tttttctaac tatatgaatt gatttttcat
    75421 atgttccttt agtgggagca ataacgagta tttctttcat aataatccct ccagatttca
    75481 tcaaataaac ttaaatatta tcttatgact aaattttatt agttatattt taatatttta
    75541 ctttgtattt aattataaat aaagtatttt aaaaagaaca ttataaaaga ctgtatcaaa
    75601 gttaactttt agaattataa agtttcatct ttttacagtc ttttaattta caatatctat
    75661 ttatcaaatc tatctacttt aaatagtttc atatttttat tttgtttgcc aagatataat
    75721 tcacttaaca ttatacctcc taaaattgca aagagtatgc catttgctcc ataacctaag
    75781 caataccata gatttttatt tttattatct ggaccaataa aacctaaatt gtctaaagtt
    75841 gaagcaaaac aaccacaata tttatattca acttcaatgt cttttatatc cttaaacata
    75901 cttttcaatc tttgttctaa aatatcatac ttttcattag ctaaagtttc atcttctata
    75961 tcatcaaaac ttacatcttc accacctata attaatcggt tgtctttagt agttcttaga
    76021 taattatatg ggtcacagtt atctctaatt aaaatgttat ttttccaaga agcaatgttt
    76081 ttaagtggtt ttgtggctat attaaaagta tttgatttag ttgcaaaatt tctttttgta
    76141 aataaacttg tattgtaccc tgtagctaca attaattttt taccatgaac cttgtatcca
    76201 tacgatactt cagcagtaac cttatcattt gataaatcta ctttttttac ttctgtattt
    76261 tcatatactt gaagaccatt ttttaaactt acatcaatca aatggtgact atatttatat
    76321 gggtctaatt ctcgtcctcc attctttgct atcaatcctg attttaaatc aaagctaaaa
    76381 gggtttgtag attcatcaat atattcaaca tcaaatccat tttcttttct taagttatat
    76441 tcttctttga tagccttaat ttcaagttta ttggcagtat aaagtagagt atctcttctg
    76501 gcataatcac atttatttcc atatagttct ataaatgtat ctaattcttc aagtgctgaa
    76561 gcacagagat tgtatgcttt tagagcatct tttagagtca taacctctgt taaatctatc
    76621 aaattgtcat ctaattcata ctgtaacaga gaagttgtta cgctagtgct tccatgtgca
    76681 atccttcctt tttcaagaat aacagacttt atattgtttt tagctagata gtaagcacaa
    76741 atacatccag ttacgccacc accaacaatt ataacatcag tatcaatatc atcagtaagg
    76801 tatggaaatt gtcttggaat tttattaata ttagtaaaaa taggctttcc ttttacatat
    76861 tcttgattca tatatttcac tcctaaaatt tattatgtat gagtgtatta ttttctatag
    76921 caaataatat tatacatttt aattatctag ttatatgggt atgtatagat aaaatgtgat
    76981 aacattttag cataaaacat atatataata tattcataat caccttattt taagtaatat
    77041 agtatataat gtttgttaac aagtaaaaag attgaataaa agttaattaa aaatttaaag
    77101 taggaggtaa atttatgctt tcaaaagata tagcttcaaa ggtattagaa aagtgtctaa
    77161 ttactggagg agattttgct gagatttttg aagaagacac tataaataat tctattagtt
    77221 taatagataa taaagtagaa aatgcaatag gtggaagaaa ttatggtata ggaataagaa
    77281 tatttaaagg attaaagagt atatatgctt atacaagtga caattcatta aatagtctgt
    77341 tggatgtagc atacaaagct gctgttgctt taggtaaatt ggaagatggc aaaagtgtaa
    77401 tattaaatga cagtattaaa ataaataata tacataatat aaaaatttat ccaaattcaa
    77461 taggaaatag agataaagta aatattatga aggttgcata taagagtgca aaagaattta
    77521 gtagtgatat atctcaagta agtgttagct atttggataa agaccaaaaa atattaattg
    77581 caaatactga aggtatatat gttgaagata gaagaataag aaccagactg ggaataagct
    77641 caattgcatc taagggtaat gaaaatcaaa ctggatttga aggacctgga ggttgtaaag
    77701 ggtttgaaat atttgaagaa atagatccag aatactatgc aaaagagtca gctagagtag
    77761 cacatactat gttgcatgct aaaaattgtc cagcaggtaa tatgatggta gcaatagata
    77821 atggatttgg tggagtaata ttccatgaag cttgtggaca ttctttggaa gctacatcag
    77881 ttgcaaaagg taactctgta tttgcagata aattaggtca acagattgcc tctacaaaga
    77941 ttacagctat agatgatggt acaataccaa actactgggg ctctatgaat atagatgatg
    78001 agggaaatcc agctcaaaaa aatatactga ttgaaaatgg aattttgaag tcttatatga
    78061 tagataaact taatggtaga cgaatgggaa tgaatcctac aggaagttct cgtagacaaa
    78121 actacaaatt tgctccaact tcaaggatga ctaacactta tattgctgct ggagaagata
    78181 aaccagaaga tattataaag tcaataccag atggacttta tgctaaaaaa atgggtggtg
    78241 gttctgtaaa tcctgtaaca ggagagttta attttgcagt ttcagaaggt tacttaataa
    78301 aaaatggaga aatacaagag ccagttagag gtgctagttt aattggaaaa ggtagtgatg
    78361 tacttatgaa tatagatatg gttgggaata acttaaaaca agctcagggg atgtgtggct
    78421 catcatcagg aagtatacca actaatgtag gtcaacctat gataagggta aaagaaatta
    78481 ctgtaggtgg aagatagggg ggataattgt ggaacttaaa agttttaagg atatattatt
    78541 taaaaaggct ctaagtgaag gttttgaaga gtgtgaaata tattattata caggagaaaa
    78601 tttaagtata aatatatatg aaggagaagt tgagaaatac aatcttgata aatcttttgg
    78661 tttatctttt agagggaagg taaatggaaa aataggttat tcttatacag aaattttaga
    78721 tgataaagct gttgatatgt tgataaaaaa tgtaaaagac ggagtgaaca ccattgaaaa
    78781 tgaagatgta caatttatat atgaaggtga taaacaatat aatgctgtaa agacttattc
    78841 taaagaatta gaaaatttag aggcagacaa gctgattgat ttagctctag aaatggaaag
    78901 agagactaag gcttattcag ataaggtggt aaatttatct aaatgtacta tatcttattc
    78961 tgtatcaagt aatggtatat ctaatactaa aggtcttaat ttaagtaata aaactaatat
    79021 gtttatagga tttgtagttc ctattataga agataatgag caaaaatatg atggaatagg
    79081 atataatata gcaagttcta tcgaagaaat aaaaccttgt gagatagcaa aacttggtgt
    79141 tgataatgct ttatctaaag taggtggaaa gagtattcca tcaggcaaat ataaaactat
    79201 attgtttaat gaagctatgg tatctttgct tagcacattc tctggaatat ttaatgcaga
    79261 ttcagctcaa aaaggattat ctttattaaa aaatagagaa ggagatatga tagcatctcc
    79321 tatagttaca atagttgatg acccactttt agaaaatggt atggcatcta ctccttttga
    79381 tgatgaagga gtagctacat ttaaaaaaga agttgtttta aatggaaagc ttataacttt
    79441 acttcataac ttaaagacgg ctaataaggc aggtgtaaag actacaggaa atggaataaa
    79501 atcttcatat tcatcaccta taagcatata tccaactaat ttttatatag agaaaggaga
    79561 taaatctcta gatgaaatta tgcaggatat aggtgaagga ttgatggtta ctagttttgc
    79621 aggtcttcat tcaggtgcaa attcagtaac aggagatttt tctttagctg ctaaagggtt
    79681 ttatattaaa gaagggaaaa aagtatttcc agttgagcaa attacagtag caggaaacta
    79741 ttttgacttg ttgaaagata ttgaagttat aggggaagat ttagaatttc caatgagtag
    79801 tataggttca ccttcagttg ttattaaaga gctatcagta gctggaaaag atgaataatt
    79861 aaaaagtaat tttgtggata aaaggtcatg ttaattttaa ttaatatggc tttttattat
    79921 ttaagtagat tttattatct aagttatatg aaaaagtcgt gatgcataca attaatatca
    79981 tacattaaat ttaaaggagg agatattatg agtgatgcac attacaacat gcacaagcga
    80041 aatggatatt caagtgatga gccatatcca gaaataaaag ttttaggtcc aaataagtat
    80101 tatgcagaat tattgatgga tgactatgct ggagtatcaa gtgaatttac gagtgtaaat
    80161 caatatctat accataattt tgatttagat gaaaagcata gagagttaaa tgaaatgtgg
    80221 gtaaatatat ctataacaga aatgttacat atggagatat tagcaaagac tataagatta
    80281 ttgggtggaa atcctgtata tagaggttct acaagttctt gtggtgcata ttggaatggt
    80341 ggttttgtat gctatggaga ctctatttgt aacagattaa aattagattt gcatttggaa
    80401 catgtggcta ttaacaatta ttataaagat ataagtctta ttgaagaccc ttatataaaa
    80461 gctatattaa atagaataat cctagatgaa aaacttcatg taagtttatt tgaaaaggca
    80521 atagagaagt attgcaagta aattttaaaa tttagttatt cattatttta ttcatatata
    80581 taaaataact atcatacatt ttcttgaagg gggttgctgt ttatatgtat tatgtatatg
    80641 tgaatgatat aaatatagca gtatatgatt tgaatccgag tgctaaaaaa actgtacttt
    80701 ttatacatgg ttggccatta ggacataaga tatttgaata tcaaacaaat atattgccaa
    80761 aactcggata tagaacagtt tcaattgatt taagaggttt tggtaaatct gatgcaacat
    80821 caggaggata tacttatagc cagttagcag acgatattta taaggtggta catgctattg
    80881 gtttaaaaga ctttaccctt gttggttttt caatgggagg agcaattgta cttaagtata
    80941 tgagtttatt taatggatat ggagtgtcaa aattagtatt agctgcagct gctgcaccat
    81001 catttgtaca aagacctcca gaatttccat atggtatgac acgtgaagat gtcaataaat
    81061 tgatagcaca agcatgtact gatagaccgg aaatggtaac tgattttgga gaaaaagtat
    81121 ttgcatcaaa tccaccagaa agctttagaa gatggtttaa cgatattggt tttagtgcat
    81181 caggtattgg aactattgga actgctgtat ctttgagaga tgaagaatta tttgatgatt
    81241 taaaatgtgt aagagtgcct actggtatat ttcatgggaa attagatgaa atatgtccat
    81301 atgaatttgc agtttttatg aatgaaaaaa tagaagattc aattttatat acttttgatt
    81361 atagtggtca tgctatattc tatgatgagc ttaaactatt taatcaggaa tttttacagt
    81421 ttttagagga atagttatat tttattatca cttagaaaaa gagtaatatt atgtgaagtg
    81481 atttaagcta aattttatga tgtattttac actaaacaaa tagggtttta gaacttgtaa
    81541 ctaagttctg agaccctatt attatatatt cctaaaattt gttacaattt attctttatc
    81601 tttcttaaaa attgtcgata tattaataat ttcatattaa atttttattt ctattccaat
    81661 aatgtcaaaa taatcgtttt agaaatttaa attatatgtg aaaaaagata atctacatta
    81721 ttttattgtg gaaaataata gaaaatggta caaaatttga tttttaagat ttaaaacttt
    81781 tttaaaaagt gctacaatag accaaattat tactttctat actcattaaa caggaggtgg
    81841 gactatgaaa aaacataata ttttatttgt atcaactgat gataaaataa atatagatat
    81901 atcaaaacaa ttggaaaata tatttggaga attttgtagt atagataact tgatatatgt
    81961 aaataggata aatattgaac tatcaagtta tgaacttgta gtatgctcag acaatgatat
    82021 aaaggaatat atacataata atattgataa aaatatacct attgtaataa tacatcgtac
    82081 tataaatatt gaaaatataa atcagataat aagtatagaa aatgattctg atgttatggt
    82141 tatagatgct tataaagagt cagcagatga aactgccaaa ataattagaa aactaggatt
    82201 aattcacatt aatctaatac catattatcc aggttgtgat aagtctaaat gtgagattgg
    82261 aattattaca ggaagcagaa acagcatacc acaaaacata aagcaaataa ttgacatagg
    82321 cgataaaata atcgatataa atacagttat agagattttt acaaaactaa atatatctat
    82381 agataaatta catattatta aagaaaaata tgatgaagat acagtaagtg gatacaggta
    82441 ttacacaact atgaataaaa ccatgaagag ttttttggag attatagatg aaggtattgc
    82501 ttctattgat aaattgggaa aatttatata ttgcaacaaa gtattctcta atctaattgg
    82561 aatagaccaa aatgaaatta tatctaataa ttttatggat ttatttagtg acaaggttgt
    82621 caaaaaaata ttttttcaag aggatgaagt taatgatgaa gtagtaaatt taaataacaa
    82681 aaaactgata attaataaag ttaatgttta tgaaaataat gaacgaataa agagtattat
    82741 aagtattaaa gatataagtg caattcaagt acttgaggac aagattcaaa ataaatttca
    82801 agcaaaaggt tttgtatcaa agtacacatt tgagagtgtt gtaggtgaga gcaaaataat
    82861 aaaagaaaaa attaatattg caagaaaaat agcaattaca gacttttctg tgttgatact
    82921 tggagaaaat ggaacaggaa aagaaatatt tgcccaagca atacataatg aatctctaag
    82981 aaaaaacaag ccattcgttg cagttaatct atcttcttta tcagatacac ttattgaaag
    83041 tgaacttttt ggttatgagg aaggaagttt tacaggagca ataaaaggtg ggaaaatggg
    83101 tatatttgaa agagcacata caggaacaat ttttttagat gagataggtg atatatcttt
    83161 agatgtacag caaagattgc taagggtatt acaagaaaaa gaagttatga gaatgggtgg
    83221 aagtaaaatt ataccaatag atgttagaat tatagctgca acaaataaag atttaaagaa
    83281 aaaaattaaa gaaggttctt ttagagagga tttgtattat aggattaatg tattacatat
    83341 agaaatacct agacttagag agcgtaagga agatatatct cttataagta agtatttttt
    83401 agatgaaata aatagcaaca aatgttttac ggaagaatca atgaaggctt taaaattgta
    83461 tgaatggcca ggaaatgtaa gagagcttaa aaatttagtt tattatattg atactatagt
    83521 tgaggaagat agagtagatt atgagcattt gccagaacaa tttagatttg aaaaaaacaa
    83581 tactttagtt aatgaaaatt ttgactcaat tatcctagat ttcaaacaat cgaatttctt
    83641 tgaggaaagt atatgtatat tgactagtgt agaaacttgg aataataaaa atatattatt
    83701 aggaagaaat aaactgcaag aaattttaaa agaaaaaggt atagttttaa gtgtagacca
    83761 aattagaaaa agaatagata aattaaagag tcatggttta cttttatctg gagtaaaaaa
    83821 acaaggtagt tttataactg atgagggcaa gaactttata tcatatataa aatttaaggg
    83881 agtgatatag ggggctaaaa aggcaaaata ccctaaaaga atccctatag tagatagaaa
    83941 taaataaaat tatttttaag attaagataa ttcaaaaata ttgaataaat aatctaaaaa
    84001 gattattttt aataaaacag tagtaaatat caactttact accgttttta ttttttgaaa
    84061 gatttattct acaattaaaa tttatatttt ggcatatcaa ttgctatata gaaaaaatat
    84121 aaaaaattgt agaacttgga ggatattgtt atgaagtcag cttataaagg atatgaagcc
    84181 tatagttatt tagaaaaagg aaaagatatt tcatctattg agatgtgtaa aggagataaa
    84241 agagttaaag aataccttat tgaattagat gatattcaag aatctagggt taaaagtata
    84301 gttgatgaaa aaatatttat atcattacat gaacatccag tgttatttcc agaataccta
    84361 gaaagagata tatttgaata caatagagaa ggtaaacaaa gatgtgctta tgaagcatta
    84421 tcaagaggat attatgatgc tatatttgat aatttaatgg atggtgtatg tacgataaca
    84481 tcaaattcag gatggaaatg ggatgatata ttaatagatt tgggtatgag actatgtgat
    84541 ttagctcatc aagattttgt cataaagtgt gaaaaagtag aagatatata cagagctaaa
    84601 agtgaaggta aagtggcatt gatacctaca ctagaaggtg cagcaccaat agaaaatgaa
    84661 cttgatagaa tagatatact atatggattt ggtataaggt taatgggtat aacatacagc
    84721 gaatccaatg cattaggttc aggactaaag gaagaaaaag atggaggtct tacaaatttc
    84781 gggaaaaaag ctgttgaaag aatgaataaa attggaatgg caatagattg ctctcacgtt
    84841 ggagttcaaa ctacacttga tgttatagaa tatagtaagg acccaataat attaagtcat
    84901 gttggggcaa gaagtttatg gaatacaaag agactaactc cagatgaagt attaatagca
    84961 tgtgctaaaa aaggaggggt tataggcgta gaagcagcac ctcacactac aataacagaa
    85021 aagaataaag aacattcaat agagtcattt atggaacatt ttgagtatat taaaaattta
    85081 gtaggaatag accatgtagc ttttggacca gatacagtat atggtgacca tgtaaaatta
    85141 cactcagtac tagctactaa atttgatata aaaaaaataa caggtacaaa agaacatcca
    85201 aaagtagaat atgtaaaagg tcttgagaac ccgacagaaa cttcaaaaaa cattgtcaga
    85261 tacttagtaa aacatggata tagtgatgag gaaatagaaa aagtactagg tagaaatata
    85321 cttagagtat taaagcaggt ttggaaatag atatattaga aatattaaca aaatctgagg
    85381 atttataggg aggttttata aatggattat ttaaaattag ctaatcaccc acttttgtgg
    85441 atagcgagta caatagccgt atcaatagta gtggtacaat cccttatatt tgctataaaa
    85501 tcttgcaaag ttggtaaaac aatgggaata acagataaac aaataaagtc agctgttaag
    85561 agtagtgcta tttcagctat tggaccttca atgactatat ttgctggaat ggtatcactt
    85621 atagtaacta tgggtggtcc tatagcatgg atgaggttat cgtttatagg ttctgtaatt
    85681 tttgaatcaa tgtcagcagg atttggaaca gatgcattag gtataacttt aggttctcct
    85741 gaaatgacaa aacttgcatt tacaaatgca gtttggacta tgatattagg ttctttagga
    85801 tggataatat ttacattact atttggacat aagctagata agataaccaa tgtcatatct
    85861 agtggaaaga aatcatttat accaataata tctctagggg caatgttggg ttcatttgca
    85921 tatctaaatg ctgatagggt gttgcgtttt gataatggaa caattgcatg tataagtgga
    85981 atggctataa tggtaatatt gtgtatactt gaaaagaaga aaaatataaa atggctaaga
    86041 gagtgggggc ttacaatatc aatgtttagt ggaatgataa tagcctcagt aatataaggg
    86101 gggagtttta tgagcaaagt aaaatctata tacaatgaag aatatttacc ttttatgata
    86161 agatatggaa gattgacact atctttgggt attatagcag cattagttcc tggaataatt
    86221 ttgagctttg ggtttggtat tatgcctcca atatcagcat tattagcaag tacaatggca
    86281 attgtcagta tgagtgctcc aaactacata atagaaccag tttcttattc accaatctta
    86341 ggtataccag gaacttatat gagttttctt tctgggaata tatctaatat gaggctacca
    86401 tgttcaatag cagctcaaaa ggcagcagaa gttgaatcag gtacagaaga aggctcgata
    86461 atatctacaa taggaatagc tgtatctatt ttagtgaata taattatact aactataggg
    86521 gtaattttag gaggaagtgt attatctaaa attccagctg aagtagtaga gaaattaaac
    86581 ttaattttac cagctctatt tgggtcagtt tttggacaag tatttttaca agataaaaaa
    86641 ctaggtctag tagcaattgt aatatctgta ttgactatta tattatcaaa gcaaggtata
    86701 ataccacagt ctttagtagt tcttatatgt gtatttggta ctatattaat tgcaagagct
    86761 atgtataaag ataagcttag tgactaatta caatgaaata ataatgaatt ttcaaatata
    86821 aaatacaatg atttgattac gcaaaattat atgaagcagg aactctgtga ctttagtcat
    86881 gggaggttca gtttaaaata taaaaattac attaagacta tttaaaaact ggtttttaag
    86941 gagggaatat tttgatgtat gatttaatct taaaaaatgg atttataata gatggtacag
    87001 gtaatcctgg tttttatgga gatatagcaa ttaaagacaa cttaatagca aaaatagatt
    87061 ataagataaa tggcaacaca aataaagaga ttgattgctg tggaaaagtt ataacacctg
    87121 gatttattga ccctcatgtt catgaagaaa tagtagcaat actagatggt aaatttgaaa
    87181 agtttctaaa acaaggagtt acaacaacta taaatggtaa ttgtggacat tctataacac
    87241 catattcttc tgaaaatgta tatgaatata tgtataaaaa tggtctatta cttgaagaag
    87301 aaaaaaagta tcttatagat aaaaataaat attggaataa ttttacagaa tattgtgacc
    87361 ttatatctaa aagtggtatt tctatcaata tgggattttt gttgggacat gggactataa
    87421 gatggagtgt tatgggaggt tctaaggata ggcatcctac agaagaggaa aaaaatgaaa
    87481 ttacaaatat aataaatgat ggcatgaaaa gtggtgcttt tggaatatct acaggattgg
    87541 catatatacc aagtaagtat gctgatattg atgaacttgt tgatattgca aagcagatta
    87601 aagaatatga tggtatatat acatctcata tcagagatta cataggaaga tataacgcag
    87661 taaaagaagc tatagaggtt gggcaaaaaa gtggtgctag ggttcaagta tctcacttaa
    87721 gcccagttga aatagaagca tttgatgaga tattaaaggc taggtataat ggtgttgata
    87781 taatggttga tacagttcca agaagttctg ggcattgtat gaagaaaaaa agagtaattc
    87841 aatttattat ggcaatatca tcaagtttat ttgaattagg tatagacggg gtaatggatg
    87901 ctcttaaaaa tgaagagggt agggcactta tattaaaaga agcatttata cttggagata
    87961 gggggagtat aatacttcta aacactaagg atattgatat ggaaaagaaa tcaatacgtg
    88021 aaatagccat acaaaaagat atagatgagg acaaattatt actagattta cttttagatg
    88081 gagatgaaga gcttatcttt tgtttaggag gaatgtatag atctgatttc ccagataaac
    88141 tacatgataa taagataatt gataatccat ttgttatggt tggttcagat tgtttattta
    88201 gtgtgggtgg ggatatgtca tggtttgaac tacaaaggag aggagctttt tcaattttct
    88261 tcaatatgta cagaaagtct ggcgttagat tagaagagat tgtaagaaga gttacttcat
    88321 taccagctag gcagtttaaa ataaaaaata gaggtattct taaagaaggg ttgatagctg
    88381 atatagcagt tattgagata gagaactata gttatcctag gtcagaagat attgattttt
    88441 caaagccaca atcactggct aaaggtgttg aatatgtaat tgtaaatgga aaaattgcat
    88501 tagaagatgg aaatattact gagaataaat gtggtgaggt attaagaaga taaggtttta
    88561 aaaataatag ggatttagaa agcaaataaa tgtctaagta agtaaagacg aagtttgaga
    88621 tttatttaaa taagaatcat gcttcgtttt tacttatagt attaagattt att
//
LOCUS       DAMJVM010000004        79698 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.004, whole genome shotgun sequence.
ACCESSION   DAMJVM010000004 DAMJVM010000000
VERSION     DAMJVM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 79698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79698
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.004"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(40..813)
                     /locus_tag="PC523_000303"
     CDS             complement(40..813)
                     /locus_tag="PC523_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HDF2473875.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(813..1199)
                     /locus_tag="PC523_000304"
     CDS             complement(813..1199)
                     /locus_tag="PC523_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2473876.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDMFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1224..1541)
                     /locus_tag="PC523_000305"
     CDS             complement(1224..1541)
                     /locus_tag="PC523_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HDF2473877.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHGLIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     MEG"
     gene            complement(1553..1813)
                     /locus_tag="PC523_000306"
     CDS             complement(1553..1813)
                     /locus_tag="PC523_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDF2473878.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1813..2109)
                     /locus_tag="PC523_000307"
     CDS             complement(1813..2109)
                     /locus_tag="PC523_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2473879.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2178..4604)
                     /gene="gyrA"
                     /locus_tag="PC523_000308"
     CDS             complement(2178..4604)
                     /gene="gyrA"
                     /locus_tag="PC523_000308"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDF2473880.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFNLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4622..6523)
                     /gene="gyrB"
                     /locus_tag="PC523_000309"
     CDS             complement(4622..6523)
                     /gene="gyrB"
                     /locus_tag="PC523_000309"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDF2473881.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESH
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPVTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPAKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6524..7639)
                     /gene="recF"
                     /locus_tag="PC523_000310"
     CDS             complement(6524..7639)
                     /gene="recF"
                     /locus_tag="PC523_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDF2473882.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRVNKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRTFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKSIHVDEALLDVYDDTLARYGSYIYILRRDFIKKIASISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEISHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7656..7862)
                     /gene="yaaA"
                     /locus_tag="PC523_000311"
     CDS             complement(7656..7862)
                     /gene="yaaA"
                     /locus_tag="PC523_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDF2473883.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(7999..9105)
                     /locus_tag="PC523_000312"
     CDS             complement(7999..9105)
                     /locus_tag="PC523_000312"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDF2473884.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9347..10669)
                     /gene="dnaA"
                     /locus_tag="PC523_000313"
     CDS             complement(9347..10669)
                     /gene="dnaA"
                     /locus_tag="PC523_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDF2473885.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11325..11462
                     /gene="rpmH"
                     /locus_tag="PC523_000314"
     CDS             11325..11462
                     /gene="rpmH"
                     /locus_tag="PC523_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDF2473886.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11540..11884
                     /gene="rnpA"
                     /locus_tag="PC523_000315"
     CDS             11540..11884
                     /gene="rnpA"
                     /locus_tag="PC523_000315"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDF2473887.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEVYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            11916..12167
                     /gene="yidD"
                     /locus_tag="PC523_000316"
     CDS             11916..12167
                     /gene="yidD"
                     /locus_tag="PC523_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDF2473888.1"
                     /translation="MNKIIYILKEISRYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFIKGMYLTIRRILKCHPFHPGGYDPLK"
     gene            12199..12906
                     /locus_tag="PC523_000317"
     CDS             12199..12906
                     /locus_tag="PC523_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDF2473889.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYILAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12875..13504
                     /locus_tag="PC523_000318"
     CDS             12875..13504
                     /locus_tag="PC523_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HDF2473890.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKIKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13616..14995
                     /gene="mnmE"
                     /locus_tag="PC523_000319"
     CDS             13616..14995
                     /gene="mnmE"
                     /locus_tag="PC523_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDF2473891.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGERALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDKENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKDKTDELKKEIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVERSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15093..16985
                     /gene="mnmG"
                     /locus_tag="PC523_000320"
     CDS             15093..16985
                     /gene="mnmG"
                     /locus_tag="PC523_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDF2473892.1"
                     /translation="MKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSIA
                     LMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQADK
                     FKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVYL
                     NSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMSL
                     QEGDEKVTPFSFMNENIEKEQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPRY
                     CPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLENC
                     KIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINAV
                     RKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLTQ
                     KGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGISL
                     YEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLEN
                     KKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQVRRT
                     RGGKGE"
     gene            16978..17697
                     /gene="rsmG"
                     /locus_tag="PC523_000321"
     CDS             16978..17697
                     /gene="rsmG"
                     /locus_tag="PC523_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDF2473893.1"
                     /translation="MNNMELLKNGIEGFNIDTNDSMLEKFKIYREILIDWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDRLELTLLDS
                     LNKRINFLEEVSRVLELDNIKFIHGRAEDFGKNENYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEIDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17860..18645
                     /gene="noc"
                     /locus_tag="PC523_000322"
     CDS             17860..18645
                     /gene="noc"
                     /locus_tag="PC523_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HDF2473894.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKIQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18780..19553
                     /locus_tag="PC523_000323"
     CDS             18780..19553
                     /locus_tag="PC523_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDF2473895.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19555..20418
                     /locus_tag="PC523_000324"
     CDS             19555..20418
                     /locus_tag="PC523_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDF2473896.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETPEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLADSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20451..21593
                     /locus_tag="PC523_000325"
     CDS             20451..21593
                     /locus_tag="PC523_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2473897.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKSLEKKGIENTVVKCDYEGFLNYEDLEKAIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYNIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRAAVIALNIGNMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21729..22304)
                     /locus_tag="PC523_000326"
     CDS             complement(21729..22304)
                     /locus_tag="PC523_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HDF2473898.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGNDTSKKETKSNTNVEQ
                     PKEENSTEKEVPNNKTKPTPKEETKTQSATIYSFDVDKTDLIENKVDLSKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAEALMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22564..23856
                     /locus_tag="PC523_000327"
     CDS             22564..23856
                     /locus_tag="PC523_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2473899.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEITLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            23961..24563
                     /gene="yedF"
                     /locus_tag="PC523_000328"
     CDS             23961..24563
                     /gene="yedF"
                     /locus_tag="PC523_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDF2473900.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDKLEEGVVVTIVDNETAKENV
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNIVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24636..24899
                     /locus_tag="PC523_000329"
     CDS             24636..24899
                     /locus_tag="PC523_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDF2473901.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILIENNVDIKGIYCISKLENGSKEVEKLD"
     gene            24914..25099
                     /locus_tag="PC523_000330"
     CDS             24914..25099
                     /locus_tag="PC523_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDF2473902.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25202..26398
                     /locus_tag="PC523_000331"
     CDS             25202..26398
                     /locus_tag="PC523_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2473903.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDADGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26612..26890
                     /locus_tag="PC523_000332"
     CDS             26612..26890
                     /locus_tag="PC523_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDF2473904.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            26927..27361
                     /gene="ssb"
                     /locus_tag="PC523_000333"
     CDS             26927..27361
                     /gene="ssb"
                     /locus_tag="PC523_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2473905.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27380..27607
                     /locus_tag="PC523_000334"
     CDS             27380..27607
                     /locus_tag="PC523_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDF2473906.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27750..28070
                     /locus_tag="PC523_000335"
     CDS             27750..28070
                     /locus_tag="PC523_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDF2473907.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28078..29034
                     /locus_tag="PC523_000336"
     CDS             28078..29034
                     /locus_tag="PC523_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HDF2473908.1"
                     /translation="MNNKIRLSKAMSIIILAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFSDISYALNICIMYSLPGVVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNTFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSRLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29045..31039
                     /locus_tag="PC523_000337"
     CDS             29045..31039
                     /locus_tag="PC523_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDF2473909.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRHHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31040..31489
                     /locus_tag="PC523_000338"
     CDS             31040..31489
                     /locus_tag="PC523_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDF2473910.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRALGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31508..32836
                     /gene="dnaB"
                     /locus_tag="PC523_000339"
     CDS             31508..32836
                     /gene="dnaB"
                     /locus_tag="PC523_000339"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDF2473911.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33041..33349
                     /locus_tag="PC523_000340"
     CDS             33041..33349
                     /locus_tag="PC523_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473912.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKSEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33431..34720
                     /locus_tag="PC523_000341"
     CDS             33431..34720
                     /locus_tag="PC523_000341"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDF2473913.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKMDNISTENIKISNRA
                     HVIFPYHKELDVLSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAIRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35051..35126
                     /locus_tag="PC523_000342"
     tRNA            35051..35126
                     /locus_tag="PC523_000342"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35084..35086,aa:Lys,seq:ttt)
     gene            35133..35207
                     /locus_tag="PC523_000343"
     tRNA            35133..35207
                     /locus_tag="PC523_000343"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35166..35168,aa:Glu,seq:ttc)
     gene            35212..35287
                     /locus_tag="PC523_000344"
     tRNA            35212..35287
                     /locus_tag="PC523_000344"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35245..35247,aa:Val,seq:tac)
     gene            35321..35395
                     /locus_tag="PC523_000345"
     tRNA            35321..35395
                     /locus_tag="PC523_000345"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35353..35355,aa:Thr,seq:tgt)
     gene            complement(35777..36769)
                     /locus_tag="PC523_000346"
     CDS             complement(35777..36769)
                     /locus_tag="PC523_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2473914.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSLDDE
                     ISKIGLRLAKIVLLNPKSKDEIVKKTKENIESLKLKKEKLLTENNIPLDYLEIKFQCV
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKSSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDSNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36778..37836)
                     /locus_tag="PC523_000347"
     CDS             complement(36778..37836)
                     /locus_tag="PC523_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDF2473915.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNSSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSVKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIIKACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            37961..39424
                     /locus_tag="PC523_000348"
     CDS             37961..39424
                     /locus_tag="PC523_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HDF2473916.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNIEYINNTKSNIDKIYLHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSVGRFGYGENTINVTNWFPIVCVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLVNDKHDNKKMLYEIEAKNVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFRPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39509..40306
                     /locus_tag="PC523_000349"
     CDS             39509..40306
                     /locus_tag="PC523_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2473917.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     IDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTENDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSNAGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40319..42421
                     /locus_tag="PC523_000350"
     CDS             40319..42421
                     /locus_tag="PC523_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HDF2473918.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     VYQEYLSSLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELDSYIEIFKTKWLFTSIIVSMTSSVLFVRLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVIMISLIGSSNFQDIFYNLFSNVFNRMGTNLMSAL
                     LFIFLMHFMWFFGIHGGNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGFLLCLVIAIFLSEKRINVRKLAKIASIPAVFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTITSYVAMSTGIVPCTVSAVEWTSPIFLSGYISTGSIRGSLLQLFNLCVG
                     VMIYIPFIKISQNRYTYMFKNNIENLTKLVKRYELTGEQPNFLNHNGKIGSISKMLAS
                     DLKFAMKRGEIEIFYQPQVNYNGDVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACVDLKKSEKLLRNSVKFSVNISPDQLDEPKLPEQIKNIISSNDINPNM
                     LGIEITEQVALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENMDQRDKLYRLGCEIYQGYYYS
                     KAIPFEEFIEYANQEREI"
     gene            42489..42968
                     /gene="rlmH"
                     /locus_tag="PC523_000351"
     CDS             42489..42968
                     /gene="rlmH"
                     /locus_tag="PC523_000351"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDF2473919.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSSKEMEIIKDKEGKKILGKVKHNSYVIALAIDGKSLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSRMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43117..43428
                     /locus_tag="PC523_000352"
     CDS             43117..43428
                     /locus_tag="PC523_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2473920.1"
                     /translation="MIKIMLSCSAGMSTSLLVTKMEIAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            43476..43793
                     /locus_tag="PC523_000353"
     CDS             43476..43793
                     /locus_tag="PC523_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2473921.1"
                     /translation="MDEKIIEISFNIIGYAGKGKGLAFEAIKEAKNGNIEKAKDLLKE
                     SKEVINKAHKYQTELIQNEASGNKTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            44005..44706
                     /locus_tag="PC523_000354"
     CDS             44005..44706
                     /locus_tag="PC523_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2473922.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKETIS
                     IEKIINDTSSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLDKDILLGRKRKTYDESDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            44927..46228
                     /locus_tag="PC523_000355"
     CDS             44927..46228
                     /locus_tag="PC523_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2473923.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKATLDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVESVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISAALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSSHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGIIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            46366..47460
                     /locus_tag="PC523_000356"
     CDS             46366..47460
                     /locus_tag="PC523_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDF2473924.1"
                     /translation="MERRLGISVYPEHSKMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILEISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATSTKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVDRYQVVFNVVAKEKTSEIEKTIMVNEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNTNIFKRGDVVIGNDDFGKYKGELQLILQDCTDERK
                     NKVGRIKEDEIILLDFIEPWSKFKIILEGE"
     gene            47462..48595
                     /locus_tag="PC523_000357"
     CDS             47462..48595
                     /locus_tag="PC523_000357"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HDF2473925.1"
                     /translation="MKIDILIKGGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQANQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSNMMNIDEDELKNAVKKYKDFVVGIKARMSK
                     SVVISNDIKPLKVAKRIKNELNLPMMVHFGSSPPMIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDVAMKSKDAEIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIINKVTKNAAKAISLKNKGLIKEGYDADLTIFDV
                     VDEEKELQDSLNKSVITSISIKPRAVVVNGEYLNIGKSIRENE"
     gene            48613..49722
                     /locus_tag="PC523_000358"
     CDS             48613..49722
                     /locus_tag="PC523_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HDF2473926.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELVNKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTLKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRGGEIAIYT
                     RDYKANEGKIEIDIRSVTGGDLDIIYNKIKEIIEK"
     gene            49787..50527
                     /locus_tag="PC523_000359"
     CDS             49787..50527
                     /locus_tag="PC523_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HDF2473927.1"
                     /translation="MNCTPQYYKNRVCLNVLAGSIKNAKEVYDACDGHILVGLLSKNY
                     STVEVAIEDIKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQDDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVHIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGDTKVEDIEVLYKIMKELLG"
     gene            50532..51524
                     /locus_tag="PC523_000360"
     CDS             50532..51524
                     /locus_tag="PC523_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDF2473928.1"
                     /translation="MNILAFGEIMMRLSVPGYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANVRKLGVKDDFITYGGNHIGVYFLESGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEGMDVVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERSYDDLVNDYKKILPYCHGLFASCRDINKILNIDNLDIKSSSE
                     LIKKEFDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESKEYKLEIYDRVGAGDGYVA
                     GIIYSYLENMSNEDIVEFGVASSVLAHTTYGDNSLVSTSHVLRLMNNEKIDILR"
     gene            complement(51712..52593)
                     /locus_tag="PC523_000361"
     CDS             complement(51712..52593)
                     /locus_tag="PC523_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2473929.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKSLEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEHK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVASNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            52866..54254
                     /locus_tag="PC523_000362"
     CDS             52866..54254
                     /locus_tag="PC523_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473930.1"
                     /translation="MEDKFYAKGKGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLVYRVIDIINPTNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLIILDVEDLTRPKCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKSWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFLGPRLAVTEDIIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            54282..55235
                     /locus_tag="PC523_000363"
     CDS             54282..55235
                     /locus_tag="PC523_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2473931.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSICVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNVIGTKVAEATGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTKLAQVRQLGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYIEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTPEFKERKTAYNFIEPLTKEKQAGASLR"
     gene            55288..55512
                     /locus_tag="PC523_000364"
     CDS             55288..55512
                     /locus_tag="PC523_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473932.1"
                     /translation="MNEKPSFKQIEIILVVICLVFAHYNVRLTPEFIAIIIPLYLSYF
                     IVSIIANQIAIKLLEIRQTREATSYNIGNG"
     gene            55505..56017
                     /locus_tag="PC523_000365"
     CDS             55505..56017
                     /locus_tag="PC523_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDF2473933.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMEYMNFNQNLELFLTLSILGFFAVVIAS
                     SFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAIAMAVGIWYVGVGVDLFTRY
                     ILSKDKKKLN"
     gene            56049..56903
                     /locus_tag="PC523_000366"
     CDS             56049..56903
                     /locus_tag="PC523_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HDF2473934.1"
                     /translation="MVMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDIENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGVSKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVA
                     ELWFEGEEEWYKAVVEEAKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            57529..58050
                     /locus_tag="PC523_000367"
     CDS             57529..58050
                     /locus_tag="PC523_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2473935.1"
                     /translation="MVLLIVDTQKLITNEELYNFNEFVVNLVKLIDTARKKYIEVIYV
                     RHDDGIDCELTKGKHGFEIYEEFKPCDEEKIFDKDVNSAFKETGLLEYLTNKNEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPSYANTTIDNSFMSAEQSYKYYNEFIWNGRYA
                     ECISLDETLKRMN"
     gene            58539..60620
                     /locus_tag="PC523_000368"
     CDS             58539..60620
                     /locus_tag="PC523_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2473936.1"
                     /translation="MDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKINDM
                     LKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKAERLLYMYLMLFIN
                     MEDISINHFINSIKVSRSTVNLDFKDLIPNLEKKGIEVKNNRINGYYLVGNEMEIRRV
                     LIKSIIETLSNEKSSRVFDIFIKEYNLDSFEEDKEIILKLIKKYEITFVEDRLIEFIY
                     IFIFLKARMYSTRIVTHENVDIPNIAVMSSMKEYKFAKELLETYESEDKIKSHNIMYI
                     SAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFRPA
                     YYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHFAT
                     LFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENMSEP
                     VDIVFTTNYNTEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEVMN
                     IIKKYANVTSESILSSELLTYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNWED
                     AIRQSASVLVENNKTTEGYVEAMINSAKASGPYIVITKHVALPHARPEAGSKEIAIGI
                     ATLDSPIEFGNDENDPVKYVFCLSAIDNNSHLRAMSELVELLEEEEFFKVLDSAKEPK
                     EIIDYIKTHEL"
     gene            60635..60922
                     /locus_tag="PC523_000369"
     CDS             60635..60922
                     /locus_tag="PC523_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2473937.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIVKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKHLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            60949..62220
                     /locus_tag="PC523_000370"
     CDS             60949..62220
                     /locus_tag="PC523_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDF2473938.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFITAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNSSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            62277..62876
                     /locus_tag="PC523_000371"
     CDS             62277..62876
                     /locus_tag="PC523_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2473939.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACNLIFEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTPAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKGTLETLKINGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIVLQSLSVALQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            62881..63765
                     /gene="dapA"
                     /locus_tag="PC523_000372"
     CDS             62881..63765
                     /gene="dapA"
                     /locus_tag="PC523_000372"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2473940.1"
                     /translation="MKYKGIICAMITPFDENQNINSRATCQLIDYLIKNGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVIDLSKRMEAVGASALS
                     IITPYFVTPTQQELILHYETIANSVNLPIIMYNMPDKTGINIEPESVCKLSKIKNIVG
                     IKDSSGKLDNMKAYIEVTKNEDFSVFSGSDSLILDSLKAGSQGAVAATANFLTKIDIS
                     IYENFMRGDLEEAQKAQSSIEEFRRILKLGTIPSVLKKTIVLNGIDVGTARLPVIEPT
                     GDALEEIKNVVENYREMF"
     gene            63894..64526
                     /locus_tag="PC523_000373"
     CDS             63894..64526
                     /locus_tag="PC523_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HDF2473941.1"
                     /translation="MKKMNITKRMAECGALAIVREEKLNRACEIVEGCIKGGITVVEM
                     SYTLNNAGEIIQGLNKKYGEALCVGAGTVLDSETARHAILHGAQFVISPNYNEDVAKI
                     CNRYQIPYAPGCTSLTESVEALSLGAAFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINLENLHVWLENGVDVCGFGGLLTKGTVEDIAKNAKKIREIIKNIREV"
     gene            complement(64712..65989)
                     /locus_tag="PC523_000374"
     CDS             complement(64712..65989)
                     /locus_tag="PC523_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2473942.1"
                     /translation="MENTVKNSKIFYGWWILLACIIVMALGYAPIVSLASLFIKPITE
                     DLGFTRSAYTLSTAIISLMCMFMASITGKLMSTKHMHLIHVICVAGLSIFYGLYSLAT
                     SIFHFYVVSFFIGLFTYGATKMPVSILISNWFYEKRGLALSLSMAGTSISGTLLSPLV
                     VKLITNYGWRQTYVYLAIVMFLIMVPATIFIVRQFPSCKGLQPYGYDRHQDNTNKNTI
                     KNEWNISLKEAKKLPVFWTFTFGVTLILLTGCILGHIPAYLEDSGYNPSLTASIVSTY
                     LFIAIWGKVVLGHVFDKLGSKAGVLLGNICFIISIVCLLFIEFKPMLYLMTFFFGIGT
                     CIGTVTTPVLTSRIFGTKHYPEIYGFVASCTCIGSIIGTPAIASIFDLTGSYKMAWII
                     LIILSIIMTLALVYSINEGHKYRLKDVSQQTIK"
     gene            complement(65991..67181)
                     /locus_tag="PC523_000375"
     CDS             complement(65991..67181)
                     /locus_tag="PC523_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2473943.1"
                     /translation="MSDMFMNLAKQFESEIISWRKHFHENPEIGMELPNTVNFIQKKL
                     EEFNIEYKVGCNGYSITGLIGNKNSGKCILIRSDMDALPVIEQTELEFKSNNNYMHAC
                     GHDAHMAILLGAAKILKLKENELEGSVKLMFQPGEEVFEGAKAMISEGVLENPKVDSA
                     FGMHVVPTMPNKSVVYGKEFMSSCYGFKITIKGKGGHGSQPENCIDPINTGVHIYLAL
                     QELISRECPPQELATLTLGQFKSGEACNVIPNEAVLQGTLRTFSPTTRDLLINRINEV
                     VQSVSITYRCSADIEVLSNVPALICNDELNKKFMSYIYELDKNIKMSEFNHTMGSEDF
                     AYISEKVPTCFMYLGAGIEDTSKWYVAHNPKVMFNDKCLALGSAIYAQCAYQWLKNMS
                     KESL"
     gene            67366..68244
                     /locus_tag="PC523_000376"
     CDS             67366..68244
                     /locus_tag="PC523_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2473944.1"
                     /translation="MNLNYFQYLVEVANIGSISKAAEKNFVSQPYLSGIIKKMEDEVG
                     FLIFKRTNLGIELTEKGEIFINEAKKILIGIENIKNINTKNEEGLSIGMVYSLFFTKC
                     FLKFKKFKPTDLQDKVYEMSPMEIFKALIDNKIRLGLFSYATKKKDKYWNIAKKHHLK
                     CWSIYEDLHPYVIMSRSHPLALNDSITISDLNSNPYVVYDKSEFLIYLDMIGIRKHPD
                     LLLVSDRGSFSDAILDNNYLGITTYFSEINEQDNKFIYIKLEDCDETIGIGYAILDNY
                     KLSQREKDFIKFIKNK"
     gene            69023..71530
                     /locus_tag="PC523_000377"
     CDS             69023..71530
                     /locus_tag="PC523_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2473945.1"
                     /translation="MKEDIGLHRMCKEEQVTKNEVISEDILKSMSMGVSKHFLDDSFT
                     ILWGNTSFYQLFGYTEEEFLFKFSSLKDYYEQNLYNFESMKNHFLEAYHRGEKSTEYQ
                     ICISANNVDPVWVIMNGILIESEENEKSMVYILYSRINELEIKQEEASKMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKQSCNILQASVNELIGRKCYEVIQDRTSPCPFC
                     TNPYLKKNDVYEWEFYNKKLKRTFMIKNRMLNWKGRRARIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRVDARDYLTILWYNDIFLNMIEYTKEQFAEELDNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEARIYTRSREERIWTVTLCYVSGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQYISEKDALTGIYNRAETERQIKKYFEENLNSMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMCDSDVVGRIGGDEFTIFMKDISSVKDAEKKAEEL
                     LYMFRHLFQKEKSSLKVTCSIGIAIYPKDGTTFKEIYTRADKALYQAKNIGKNNYVIY
                     NQDSFKELEAFNCSSLGTVIDSEKQYAECPDNLTRYIFRMLYQTDNIDETINMILEVV
                     GKQFNVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNNNYQDYEDYEKLFGDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDKVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNKKFNQQMQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKLQIGQPCYLDAVDIHKISIAWNQKEAYLCISMI"
     gene            71873..74041
                     /locus_tag="PC523_000378"
     CDS             71873..74041
                     /locus_tag="PC523_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDF2473946.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKFNKNYSNWIKDIADENNVTISKYVFNNNEKLSIYTTDTSLNNNIKLKSGEFPN
                     TNTNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFDKISEVGESGILYISTQNKDT
                     INKILKELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSR
                     EASILRLNGYSKLDIITRVIRSILRIMILSSITSLLIYIIYILKLNLGVRACLYFVIF
                     SIICILINIFISILIVGFNIRSSKYVLTLNGKKPYGFVNIIHFTLKIVFVSFLILSIN
                     NCILNYNILQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKSLVE
                     EKDAFLIDTTNFEKLENGSYMYEINSKGKKPEVSQYGKNIKINKNYLKVNPIYSNGKE
                     IFNLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNEELNRDLNKTK
                     KSDLNVNIIYVDNNQSYFTYNSLVMDDNRNLINDPIAIVDIDNIDDSFYLSYISRCVY
                     FNSKKLDALADISKIIEAQGVEANIQSLRAVYNEYGLEINKLEKYLNSEIFTIIIIAI
                     SNLMITYNIVVSYYERNKYKLYIKKIFGYSATARSILLIISLILTNVIPIGIISTRVD
                     LSNNIVLFGLLILVVEVIISIILDKIISNSSFNKIIKGEH"
     gene            74046..74663
                     /locus_tag="PC523_000379"
     CDS             74046..74663
                     /locus_tag="PC523_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2473947.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVESNLKIAL
                     KYQKNINRDEKINYVLESVGLGKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNNNGKTIVLVTHDLELSEQVDKIIKL"
     gene            74852..75196
                     /locus_tag="PC523_000380"
     CDS             74852..75196
                     /locus_tag="PC523_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDF2473948.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLELEGDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSAIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVIKKA"
     gene            75768..76634
                     /locus_tag="PC523_000381"
     CDS             75768..76634
                     /locus_tag="PC523_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2473949.1"
                     /translation="MISIHKNIYDDKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCMNLQPDFKCSVHDNLMKRGFKGCTAYDCFGAGQKVAQVTYKGIDWMQSPELA
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKINLRIEETEKITNMSSEQIVDLD
                     IISHREKVNSLLLQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKTNLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PVSLIRPKYWGK"
     gene            76747..77151
                     /locus_tag="PC523_000382"
     CDS             76747..77151
                     /locus_tag="PC523_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HDF2473950.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNEYYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEVIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            77327..77776
                     /locus_tag="PC523_000383"
     CDS             77327..77776
                     /locus_tag="PC523_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2473951.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIKFLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKAFDRSEIDGQGNPFPILHMKLI"
     gene            complement(77933..78310)
                     /locus_tag="PC523_000384"
     CDS             complement(77933..78310)
                     /locus_tag="PC523_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2473952.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLESNPNNPVMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSRETIEEISIKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            78572..79489
                     /locus_tag="PC523_000385"
     CDS             78572..79489
                     /locus_tag="PC523_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HDF2473953.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYIYELMKPFEFKWKC
                     MNVPIVAKQKGGYDVIIASEMLGVLSPKDIDEKQKKNINMLSTDKIWNTCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGIAENFAKWMFGEEMVGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVEEFWE"
BASE COUNT        30435 a         9564 c        13178 g        26521 t
ORIGIN      
        1 catctttaaa acagtaggtc aatacttttt tatatttttt tataaatttt tttcatattc
       61 ttttttcagc ttctctacta cctttttctc tagcctagaa acagtcattt gagatatccc
      121 taattcctta gctatactag attgtgtttt atcaaagaaa aatctatctt taaaaatttt
      181 aacttctaat tcatttaaag tttcaataaa cttattaata aaatcttcat actctatact
      241 tccaaagtta ccttcttctt taccaatctt atcaataaga gttatatctt tatcctctga
      301 atcatcatta cttgatgaat ccaaagacat tggttgatat ccataagaag cctccatagc
      361 ctctagaaca tcttcctcac taacgcctat ataatcagct atatctttta cctttggatg
      421 ctttttattt tcttgttcta atttaatctt cgcatcactt atctttttgc ttaattcctg
      481 tatccttctt gggactctaa gtgtccacac cttgtctcta aaatactttt ttatttctcc
      541 tactatagtt ggtgtcgcaa agctggagaa ctcaaaccct ttctcaacat catatctatc
      601 aatagcataa attaatgcca atgaagcaac ttgatatata tcttcaaaat caacaccttt
      661 gtttatatac ttcttagcta acaatcttgc taaatacaga tgtctttcaa taagaatatt
      721 cctaatatct acatttttat ttttattata taaattaaat agctcttttg tatccatatt
      781 taggtaatgt gtagcattag ctacattttt cattaaatat caactcctaa atatttagtc
      841 atttttattt ttgtcccctg attatgttct gattcaatat ctacatcatc cattaaagtt
      901 ttaattataa ataatccaag accactctct tttggattct ctaaatctgg tgtacttatt
      961 gagctaacat catatccttt tccattatct tgaatttcta tattaattgc attatctaaa
     1021 atattaaata ctatattaaa catatcatct ttactatgtt ttattgcatt tgtacatgct
     1081 tcagatactg ctacttttat atcttctata tcatctattg gaaaacccat cttattggca
     1141 attccagagg ctgttaatct tattatacta acataatctg gatttgtagt aatttccatc
     1201 tttatagtct cacaagccaa agtttatccc tccattttaa atattttatc tagacctgtt
     1261 atagtaaaaa tctttcttac attactctta ggatttacta tgtatatctc tttttcattt
     1321 atttttaatt tttttaatac acctatcata gctcctaaac ctgttgagtc tatataatct
     1381 agatctttaa gattaatctt tacatctaac atattctttt ctattaaacc atgtaagtgt
     1441 tcctttagtt tatctgctgt agaaacatca agttctccat caaggcaaac attccaaaat
     1501 ttattttgtg aatctaaatt tgaatcaata ttcatagaca tgtaaaaacc tcctatttcc
     1561 ttttaataat tttaaatata tttcctatac tcaatatttt attaattaca taaactatat
     1621 catctattcc ctttgatatt gatgatgcat tttcaacaat atcttgtata tgtctttcat
     1681 tataatctac aatcttatat gccttttcaa caactttatt tatgctattt agagttttag
     1741 ctaaataaat tgctactatg agagcagagc ttcctactaa cactgctcca atctgccacc
     1801 cccatgcatt cattagttaa catctccctc atcatcaaag cttttactta ttactatatc
     1861 ttcttctgaa atctttatat cttcttcatt attatcatcg tctactaaat ttattattct
     1921 ctctttaaca tcattaaatg tttcatgtag aacttctttt ggattttctt taacatcatt
     1981 aaacttctct tttgtttctt ttcttagttc agaacctttt ttgggtgcaa aaaacatacc
     2041 aaatataaac cctaaaatag ctcctaataa taaaaatcca ccttttttac aacttctttt
     2101 attgcacata acaattactc ctttcaattt taaattttta tctaactaca ttatacaaaa
     2161 agaataggat atttatccta ttcttcttca atatttattt tggcaatcga aactacatta
     2221 acttcatcat tcgtcttcat taatttaaca ccacttgtaa ctcttccaaa cagtgatatt
     2281 tcattgactc ttattctaat aagaactcca tcagaattta taatcattat ctcatcatct
     2341 tcattaacca tatctgcacc tacaattgta ccagtctttt cagagatatt ataagtttta
     2401 attcctttac cagctcttat ttgacttcta tactcttcta tatttgtcct ctttccaaaa
     2461 ccattcttac ttacaactaa cacatctgta cctttactgc aaaggttcat agacacaaca
     2521 aagtcttctt tacttaaagt tatacctttt acacccatgg ctgttctacc catatatctt
     2581 atatcatttt catcaaatct tatagacata ccttctttag ttactaaaag tacttcttgc
     2641 tttccgtctg taagttctac tccaataagc tcatcatcat ctcttaagcc tatagcaata
     2701 agacccgatt tatttatatt tttgaactct tccctcttag tcttcttaac aatacctttt
     2761 ttcgttgcaa gtaataaata ttcattttca tcattaccat caataggtat tagagtagca
     2821 attttctcat ctgctgacaa ttgaagtaaa ttcactatag cagttccttt agcctgtctc
     2881 ttaccttcag gtatttcata tgcatttaat ttaaatactc tacctttatt tgtaaagaat
     2941 aatagtctac tatgagtagt tgtagttact aagtgtctta caaaatcttc ttctctagtt
     3001 gtaagtgctg aaatacctct tccacctctt ttttgactct tataagtatc agatggaagc
     3061 ctttttatat atccaaagtg agtaagagtt attgctattt cttcatcact tataagatct
     3121 ctcatatcta tttcgccttc agcatgtctt atttctgttc ttctctcatc agagtaattt
     3181 tcttttatta ttgtaatttc atcctttatt acattcaaaa gtaatctttc atcagctaaa
     3241 atttctttta atctatttat cttcttgatt aagtcttcat attcagcttc tatcttatct
     3301 ctttctaaac ctgtaagtct ttgaagtctc atatctaata tagcttgtgc ttggatttca
     3361 gttaagttaa atttttctat taaacctaac ttagcttctt gcccagtctt tgaagctctt
     3421 atcaagctta taacagcatc tatattatct aaagcaatct ttaatccttc taaaatgtgt
     3481 gctcttgctt cagctttatt tagttcaaac ttagttcttc ttgtaacaac atcttcttga
     3541 tgtttaatat aatgatataa tatttgtttc agatttaaaa ctcttggctg accatctaca
     3601 agtgcaagca ttattatact aaaagtatct tccatttgag aatgtttata taaattattt
     3661 aatactatat tagcattagc atccctcttt aattctataa caattctcat accatttcta
     3721 ttactttcgt ctcttaggtc tgatatacct tctattctct tttctttaac taactcagct
     3781 attctctcaa ctagtttagc tttatttact tgataaggta tttctgtaac tattatttgc
     3841 tgttttcctt ttggtagttc ttctataaaa gctctagacc taactttaac ttttcctctt
     3901 ccagttctgt atgcttctgc tatactttct ttccccatta taattgcagc tgtagggaaa
     3961 tctggtcctt gaacaaattt tattaaatca tctacactac actctggatt atctatcaaa
     4021 tatacagttg catcaattac ttctgctaag ttatgtggag gtattgaagt tgccatacca
     4081 acagctatac catttgagcc atttactaat aaattaggat atctcgctgg caacactgaa
     4141 ggctctttta atgactcatc aaagtttggt ttaaagtcta cagtttcctt ttcaatatct
     4201 ctcagtagtt ctaatgataa tttactcatt ttagcttctg tataacgcat agcagctggt
     4261 gaatctccat caacagaacc aaagttacca tgaccatcta ctaaaagtgc tctagttgaa
     4321 aaatcttgtg ccattcttac catagcataa taaacagcag tatctccatg aggatggtat
     4381 ttacctaaaa cgtccccaac aatacgagct gacttcctgt atggtttatc tggagttaaa
     4441 tttaactcac tcattgaata tagtattctt ctatgaactg gctttaaacc atctctaaca
     4501 tcaggaagag cacgtccagc tatgacactc attgaataat caatataaga ttttttcatt
     4561 tcttccgcta tttcaatagg gagtattttg ttattttctt ccatgtatac taatcccctc
     4621 tctatatatc taagtttcta acaaatcttg cattttcttc tataaattct tttcttggag
     4681 caaccttatc acccataagc attgaaaata cttcatctgc aattgcagca tcctccacag
     4741 taacttgtaa taaagttctt gtttcaggat tcatagttgt ttcccataat tgctctgcgt
     4801 tcatctctcc aagacctttg tatctttgaa gttcaacacc atttcttcca atttcatcta
     4861 ataagatatt taactcttta tctgaataaa catagtgctc cttcttttgc tttgttactt
     4921 tatataaagg tggttgtgct gcataaacat acccttcatc tataagaggt ctcatatatc
     4981 taaagaagaa tgttaataat aaagttctta tatgggctcc atctacatcg gcatcggtca
     5041 taattataat tttatgatac ctagccttat ctatatcaaa atcttctcca ataccacaac
     5101 cataagctgt tatcatattt tttatttcat ctgaagataa tatcctatct agtcttgact
     5161 tctcaacatt aagtatttta cctcttaatg gaagtatagc ttgactattt ctgtctctac
     5221 cttgcttagc tgaacctccc gccgaatctc cttcgactaa aaatatttca cttttagctg
     5281 gatctttttc tgcacaatct gcaagttttc caggtaaaga tgtactttct agtacacttt
     5341 ttctccttgt taattccctt gctttttttg cagcttctct agctctttgt gctcttaagg
     5401 ctttatcaac tattattctt gctgttactg ggttttcttc taggaaagaa cctaattcat
     5461 caactgttac actatcaact atgcctctca taaagctatt tcctagcttt gttttagttt
     5521 gaccttcaaa ttgaggttct ggtaatttaa ctgatactac tgctgtaaga ccttctctta
     5581 tgtcttcacc aagtagatta acatcatttt ccttcaaaaa cttatttctc ttagcataat
     5641 catttacagt tttagtaaga gctgctttaa atccacttaa atgagaacca ccttcgtgtg
     5701 tatttatatt gtttgcaaaa gaatatatgt tctctgtata accatccgta tattgcatag
     5761 ctagttcgac aaaacaatca tcaacctttt tatctatata aactatgtca tcatgtatac
     5821 cagttctaga tttatttaaa tacttaatat attctaccaa accacctgta tagtgaaatt
     5881 cttttctttt ttcttgtcct tctcttttat cttcaaatac tatttttatt cctttattca
     5941 agaaagaaag ctctctaagt ctatattcca atgtttcata tttaaactct atttcatcaa
     6001 atattgtctc atctggcata aattgaatca ttgttccttt atcgtgagat tctccaacta
     6061 cttcaagctt agatgttggt aatcctttct catatgtttg cttatatatc tttccattta
     6121 agtaaacctc tgctaccatc cattttgaca atgcattaac tacagaaaca ccaactccat
     6181 gaagacctcc agatacttta tatcctccgc ctccaaactt tcctcctgca tgaagattag
     6241 ttaatactgt ttctaaagtt gatttgccag ttttaggatg tatttcaaca ggtatacctc
     6301 ttccgttatc ttttactaaa acactgccat cttcattgat agatacatat atgtctgaac
     6361 aatatccttg taaagcctca tctatactat tatctacaac ttcataaact aagtgatgta
     6421 aacctctagg gcttgtacta ccaatataca tacctggtct ttttctaacg gcttctaatc
     6481 cctctaatac ttgtatctga cttgcaccgt attcttgttt catttaattt cctccattct
     6541 ctatgctaat aacatcgcca ttttcaatat taaatattgt aacatttttt tcatcaaata
     6601 tttttttatg tgaaatctca gcacttgtta taaacatttg aacattgctt aaattattta
     6661 ctaaaagttt ttgtcttgct tcatctaact cactaaaaac atcatctaaa attaacactg
     6721 gatactcttc aacctcattt tttattaatt ctatctctga taactttaat gatattgaag
     6781 cagttctttg ttgaccttgt gaaccaaaaa gtcttgcatc taaatcattt atgaatatat
     6841 ttaagtcatc tttgtgtata ccatatctgg tagtcttaga ttctatatca ttaggcctat
     6901 tcgatgacaa cttggctaaa aatttattat atactgtatc tatagtatct tcgtcagtta
     6961 tatttatttg atttttgtat cgaattgata atctttcaac tccattagtc aaattcatat
     7021 gcatattctc agatatacta gctatctttt ttataaagtc tctacgcaaa atataaatat
     7081 aacttccata ccttgctaat gtatcatcat atacatctaa taaagcttca tcaacatgta
     7141 tacttttaag caccctactc ctttgagaaa gtgttttatt ataattggta agatatttat
     7201 aatatttagg tataatctga ctaatctctt tgtctataaa tgtccttctt tctttaggac
     7261 cctctttaac caacctcaaa tcctcaggag aaaatataac aacatttaag ttgccaagca
     7321 gttcttgtat cttttgcaaa ggaactttat ttactcttat ccccttttta tcttttccaa
     7381 taattagctc tattagacta tatttattat attttgaaaa acttcctcca atatataagt
     7441 tttcactatt aaatctaacc atttctttat ctttgttagt cctaaatgat tttccaaaag
     7501 aaagcatata aatagattct actatatttg tctttccttg accattttta ccaacaagta
     7561 gatttacttt cccattaaat tccagatgca actttttata atttctaaaa ttaacaagtt
     7621 gcaaactttt aagtttcaca aaaagttctc cttttttaca atacttttat tttagttcct
     7681 tctatttcaa ctatatctcc tgattttatc ttttttcctc ttcttaattc tatttcatca
     7741 tttactgtca ctaaaccttc ttgaattaaa aattttgcat gacctcctgt tgatgcaatc
     7801 tctgctaatt taagaaattg atctaacttt atatattctg attctatagt tatttcagtc
     7861 ataatagttc ctccttgtca ctattttaaa caaattccta ttgatacact aaaagtatat
     7921 tataccataa atatgcttat aaaaggagtt gtctaagatt agaaaaattt tctacaaaag
     7981 acaactcctt catgtctgct atatatttga tgatattctt actggaagca gtaagtaaat
     8041 ataattaaca tcatctgttg gtttaattat acaaggattt acatttgtag taaattctat
     8101 aaagatttcc tcattatcta tatttttcaa accttcaata aaatatctag aattgaaagc
     8161 tatgtctaga taatctccat ctaaatctat ttcaacctct tcatacacat ttcctttttc
     8221 tgtattagaa gtaattgcca ttaccttatc tcttatagat aactttataa gattattttt
     8281 ttctgactga gacaataaag acgctctctc tatactattt aaaagctcct tagtgtttaa
     8341 tttaactctg ctgttatgtt ctcttggcaa taacttttta taatcaataa aatctccttc
     8401 aagtaatctt gtgataattt tagtatcatt aattataaaa atagcatttt tttcgttaaa
     8461 cccaacttta acattatctt ctccagaaag caaactattt acatctataa gtgtctttcc
     8521 aggaattatg acttttatat tttcagttaa actatcaact gaacaacttt ttacagctaa
     8581 tctatatcca tcaattgcta ctaaatttaa gtttctatct acaatttcta aaagttctcc
     8641 cattaaaaca ggctttgttt ggtcctgtga gatagcaaat acggtttgtt taatcatatt
     8701 ttttaagatt tcttgaggta tactatataa atcttcttca tttaattctg gtaatttagg
     8761 aaattctttt gctgcatacc ctttaatttt aaatcttgaa tttacacaat ttatgtaaat
     8821 attattttct gaatcagttt ctatctcaac gaaagaatct ggtaattttc taattatatc
     8881 tccaaatagc cttgcatcta caacaacatc tcctttttct atgatttctg cttgtacata
     8941 agtttctatt cctatttcag catcatatcc agtaagtttt aattgacctt cttctgtaga
     9001 tataagtata ccttttaata attctattgt tgtttttcca ttaatagctt tttgagcaat
     9061 tccaattcta tttgctagga ttttttgatt acaaattatt ttcaatgtgg ataacctcct
     9121 ctggcttttt atatataaaa ataagttaaa atcatagtag taatagtagt aggtgttgtt
     9181 gataatgtta ataagtatgt ataacattat aaatacaaag atattaacat gtgtataaga
     9241 tgttaatatc tttgttattt tagacttgat aatttgttaa tactttttgt tcaaaaaaag
     9301 tattaacagg ttattcaaga gatataaaca tacaattgtg tataaattat cccttcaaat
     9361 ctgatataat tttgtctatt ttagtcttaa tttcttcact ttcttctata tccttagaca
     9421 ctttgtcatg agcatgaatt actgttgtat ggtctctacc tccaaactct tcacctattt
     9481 taggaaggga taaatcagta agttcgcgcg ttaaatacat agctatctgt cttggataag
     9541 ctattgaacg tgttcttttt ttagaattaa aatcttccat ctttaaattg aatacagaag
     9601 atactttttc ttttattctg agaacattta tttcttcatt tttagaagtt gttataatat
     9661 cttttaaagc ttctgtagca agatcaaaag atattaccct attactaaga gaagaataag
     9721 caactactct agttaatgca ccttctagtt cccttatgtt tgacttaata ttttttgcta
     9781 tatatgacat aacctcattt ggcacatcta tacgttctag ttgagccttt tttctaagta
     9841 tagcaattct tgtctcaaag tctggtgctt gaatatcagt tataagtccc atttcaaatc
     9901 tcgatcttag tctatcttct agtgtaggta tatcttttgg cggtctatca ctagatataa
     9961 ttatttgttt gtttgcttca tgaagagtat tgaatgtatg gaaaaactct tcttgagttc
    10021 tttcttttcc tgctataaat tgaatatcgt cgattaataa tacatctacg tttctatatt
    10081 tattcctaaa ttcttcgttt ttgtcgtctt ttatagagtt tataagttca ttagtaaatt
    10141 tttctgaaga aacgtaaact acttttgaat cttttttttg actaacaata tgatgaccta
    10201 tagcatgcat taagtgagtc ttacctaatc ccactcctcc atacaaaaaa agtggattgt
    10261 aagctttagc tggagattct gcaactgcaa cacatgcagc atgtgcaaat ctattgctat
    10321 taccaataac gaacgtatca aatgtgtatt ttggatttag attagggtaa agaactctat
    10381 aatttaaatc tgtactatca gaatttaaat cagcaacttc tttttcgctc aatacaaatt
    10441 taatattata tttttttaat gacagctgac taatagcatc ttcaattaaa tgtaaatatc
    10501 tattctctaa gatatcttta ttaaaatcac taggagccag taatattaaa tcattaagat
    10561 gtatttttaa tggtacgatg tttttaaaaa aggtattaaa acttaccgaa gttaagtcac
    10621 cttttattaa ttgtagggtt ttgtcccata aagaaactat atccataata taaacctccg
    10681 aacagaataa ttaatatttt ccacaaagtt attaacagaa aagcaaaaat atatataaat
    10741 tttacagaat aactaaattt atggataatg gtgttgataa attaataata gtataaatta
    10801 ttaaaaaaat atgaaaagat ataaacaata tgtagataaa gttataaata acataaacat
    10861 acaataaatg gtgattataa ctagagtatt gtcagtaatc ttaaaagtta tacacaaaag
    10921 tatccacaag ctgtgaataa tttctaataa gttgataaca tttatgcaca ttatcaacaa
    10981 gtatatttat aatatcaaaa tatatgtcaa acttcaatga tttgtaacta atttatccac
    11041 aatattaact aattgttgat aaaaatataa acatgttaaa attactttat ttgtgtatat
    11101 aattgaaaaa aataatttag aataaaatta aatagatttt tactagtatt ttcaataaaa
    11161 aaaatacata atatatatat ggtagatttt gtgatataag cgtcccaaaa actcgttgac
    11221 aacagtagta aaagtattta taattatatg tgttatgttt actttgaggg taaagttatt
    11281 atttttataa acggaaaata aaatctgaaa ggcggtgcaa aataatgagt aaaagaactt
    11341 atcaaccaaa aaagagacaa agaagtaaag aacatggttt cagaaaaaga atgaagacgt
    11401 ctaacggaag aaacgtgtta aagagaagaa gagctaaagg aagaaataga ttaactcatt
    11461 aattacaaaa gggccgctgc aaaggtggcc tttttgtgta aataattaca attcatagct
    11521 gtgtttatgg aggttaacta tggactttaa taggactaaa gggttgaaaa aagactctga
    11581 ttttagaaaa gtatataaac acggcaaatc ttttgcaaac aaatatttag taatatatat
    11641 acttaaaaat aaatcagatt atagtagagt aggtatttct gtttcaaaaa aagtaggaaa
    11701 agctattact aggaatagag taagaagatt aataaaagaa gtctacagat taaatattga
    11761 tgaaaaaatt aagcctggat atgatatagt atttatagca agagtatcta gtaaggatgc
    11821 tacttttaaa gacatagaca agtctataaa aaatttagtt aaaagaacgg atatttctat
    11881 ttaattgtat gctataaata tggaggttaa tgactttgaa taaaataatt tatatattaa
    11941 aggaaataag taggtattta tcgaatttat gtatttattt agtgagattt tatcaaaagt
    12001 atatatctcc cttaaaggga cctacctgta gattttatcc aacttgctca caatattcaa
    12061 tagaggcgtt taagaaatat ggatttataa agggtatgta tttaacgata agaagaattt
    12121 taaaatgtca tccttttcat ccaggaggtt acgaccctct aaaataaaag agagtaacta
    12181 ttaggaggta taaaaaaatt gaatataata ggaaatttct taggattgat tcttaaggta
    12241 atatttgaat ttgttaatca ttatggtatt tctataatac tttttacgat acttgtaaag
    12301 attattttat taccattgac aattaagcag actaagtcaa caaaggcaat gcaagatata
    12361 caacctcgta taaaagagat acaagaaaaa tataaaaata agccagaaaa acaaaatgaa
    12421 gaaattgtta agttatatgg agaagctaaa attaatccac tatctggttg tttaccttta
    12481 ttaatacagt ttcctatatt aataggatta tttagcgttt tgagagagcc agttgctcat
    12541 ggcgtttttg caaacaaagc tgcattttta gcagctgaca atgggttttt atggataaaa
    12601 agcttaacat caccagatta tatattggca gtattttcag gagcaagtgc atatgtcatg
    12661 caaaaagtaa tgacaccaaa agatcagtta caaggctcta tgaaagtaat gacatatgta
    12721 atggcaggta tgtctttcta ttggggattt atattcccag caggacttac attgtactgg
    12781 acagtaagta acttattctc aatagctcaa tattacttaa taatgaatcc tttaaaagct
    12841 aaactggctg ctaactctaa ggaggaaaaa gttaatgaga aaaagcctaa aattaataaa
    12901 aagtaagagt aaagaggaag ctataagtaa agctatagca gagttaaatt taaaagcaga
    12961 ggatatagag gtagaaatac ttgaaaatcc aagcaaagga tttttaggtt ttataggtgc
    13021 taaagatggt acttatgaaa tttttgttat cgagagggaa gtagatgtag ctaaaaattt
    13081 tatagaggta atgttgaaaa atgcaaatgt tgatgccaaa gtcaatgtat ctcaaaaaga
    13141 taatttaatc atggttgata ttgaaggcaa agaagctgca agtttaattg gtagaagagg
    13201 ggaaacttta gattctattc aatttttgac tggattagct cttaataaaa taaataaaga
    13261 ttctcataca agagtacttg tagatataga aaattataga tcaaaacgag aagaatcttt
    13321 aataagatat gctaataaag ttgctagaga agtagcgaaa acaagaaaaa ttaaaaaatt
    13381 agattatatg aatccatatg agagaagaat aatacattca gctcttcaaa atgacaaata
    13441 tgtaattaca tatagtgaag gaaccgatcc atatagaaga ttagtaattg agtgtaaaag
    13501 ataaagtcaa cctcctaaac attagggggt tttatttttt aatattatta aatcattaaa
    13561 gattggttaa atataaatct atatgtaaaa aattatgaat taagaggtga taaatttgtt
    13621 tatagatgat acaattgcag caatagctac agcacctgga gaaggtggta taggtatact
    13681 tagaataagt ggagaaagag ctttgaaggt tgcagaagaa atctttaaat ctatgtcggg
    13741 aaagagtatt gaagaataca ataagagaac attaatatat ggaaatatag tggataaaga
    13801 aaatataatt gatgaagttt tattggcata tatgaaagga ccaaactcat atacaggaga
    13861 agatgttata gaaattaatt gtcatggtgg atttatttcg gtaaagaaga tattagaact
    13921 aatactatca aaagatgtta gattagcaga agctggtgag tttactaaaa gagcattctt
    13981 aaatggaaga atagatttat ctcaagcaga ggctgtaatt gatgttataa aagccaaaac
    14041 tgatatagct catgaagttg ctcaaaatca attagagggc tctttatcaa agaagataag
    14101 agaattgaga gataaggtca ctgagatatt agctcatgta gaagttgcta tagattatcc
    14161 tgaagaagat attgagcata taacgtatca aactcttaaa gataaaactg atgaacttaa
    14221 aaaagaaatt aaaaaattat atgatacagc tgaaagtgga aaaatactta gagaaggttt
    14281 aaagacagta attgttggga aaccaaatgt aggtaaatct tcacttctta actccatttt
    14341 aggagagaat agagcaatag ttactgatat accaggaact actagagatg ttatagaaga
    14401 gtttgtaaac ataaaaggta tacctttaaa aattgttgac acagcaggta taagagatac
    14461 tgatgatatc gttgaaaaaa taggtgttga aaggtctaaa gaatcattta ctagcgcaga
    14521 tttgatagta atggttttag atgcatctag aaagttatca gaagaagata tagagatatt
    14581 agaaaaactt aaggataaac aaacaatagt gttgttaaat aagaatgatt tgaaacaaga
    14641 aattgaggaa gaaaaaatac ttaaatatgt agaaaataat agtataataa aaatatcagc
    14701 tttacaacaa gaaggaatag aagaattaca agataaaata gagtctatgg tatataaagg
    14761 tagtatcaaa aataattcta gtttagtagt aacaaattca agacataaag atgcacttag
    14821 taaagcatat aaatctgcaa cagatgcttt aattgctttg gaacaaagca tgccatttga
    14881 ctttgtagaa gtcgatttaa aaaatatatg ggactatctt ggatatatta atggtgatac
    14941 agtaacagaa gatttactag ataatatatt ccataatttc tgtattggta agtaatgtac
    15001 aaaagtaaat ttgtttttat tatagatatt ttttaaaaat ttgtagtgtt tctcatgaaa
    15061 cattaatttt aaacagaaag gaattataaa tgataaagtt tgaagcagga aaatatgatg
    15121 ttatagtagt cggagcagga catgctggat gtgaagctgc tttggctact gcaagaatgg
    15181 gatataaaac tttgataata acaatgtctt tggattctat agcattaatg ccttgtaatc
    15241 catcgattgg ggggactggt aaaggtcaat tagttaaaga aatagatgcc ttaggtggtc
    15301 agatgggact aaatatagac aagacatata tacaaagtag gatgttaaat actgctaaag
    15361 gtccagcagt gcactctttg agagcacaag cagataaatt taaatatcat gaagaaatga
    15421 aaaagacatt agaagatgaa ccaaaccttg atatagctat ggatgaagtt gtagaaatac
    15481 ttcacgaagg aaatgttgta ataggggttg gaactaaact aggatgttca tttaaatcta
    15541 aagctgtaat attggccact ggagtttatc ttaactcaaa gatatatatg ggtgaagttg
    15601 cattttatga aggtcctaat gcactgggtt atgcaaaata tcttacagat agcttagttg
    15661 agcttggtct tagaatgaga agatttaaaa caggaactcc agcaagagtt catagagata
    15721 gtatagactt ctctgtaatg tctttacaag agggagatga aaaagtaact ccattctcat
    15781 ttatgaatga aaatatagaa aaagaacaag aaccttgtta cttaacaaga actacagaag
    15841 aaactcaaaa agttatacta gataatttaa aaagatctgc tatgtatagt ggagtaatag
    15901 aaagtactgg tccaagatat tgtccttcta tagaagataa agtagtgcga tttagtgata
    15961 aaacatcaca tcaactattt atagaaccag aagggttaaa cactaaagaa atgtatatac
    16021 aaggaatttc aactagctta ccttttgaag ttcaattaga tatgtacaaa actataaaag
    16081 gacttgaaaa ttgtaagata atgagacctg catatgctat tgaatatgat tgtgttgacc
    16141 caactcaact aaaaataagt ttggaaataa agggcgttga gaacttattt agtgcaggac
    16201 agttcaatgg gacttctgga tatgaggaag cagcagctca aggacttatg gcgggtataa
    16261 atgcagttag aaaaatagaa ggaaaagagc catttgtatt agaccgttca gaagcttata
    16321 taggtgtctt attagatgac cttgttacta aaggaactaa cgaaccttat agaatgatga
    16381 catcaagagc agaatatcga ctatatttaa gacaagataa tgcagatatg agattaactc
    16441 aaaaaggtta tgatatagga ttagttaaaa aagatagata tgaaagattt ctaaataaaa
    16501 aggctgctgt agagaaagaa tttgaaagat taaaaaatga aagagttaca ccgaaagaag
    16561 taaatagttt gctagaagaa aagggagcaa caccaataaa ggttggaata tctctatatg
    16621 aatttttaaa gagaccagag gttacttacg aattgcttga agaattagga aaaggcgctg
    16681 gagaagatgt ttcaagagaa gttaaagaac agtgtgtaat aataactaaa tatgaaggtt
    16741 atatagagaa acaattgaaa caaatagacc aatttaaaaa acttgagaat aaaaagctgg
    16801 atgagaaaat aaattattca tctatagaag gtcttagatt agaggctaga cagaaattgg
    16861 atgatataaa acctatttca ataggtcaag catctcgtat atctggtgtt tccccagctg
    16921 atatatcagt tttacttata tacctagaac aagtcagaag aactagaggt ggcaaaggtg
    16981 aataatatgg aacttctgaa aaatgggatt gaaggtttta atatagatac gaatgatagt
    17041 atgttagaaa aatttaaaat ctatagagag atattaattg actggaataa aaagatgaat
    17101 cttactggaa tagaggatga aaaagaagta tatataaaac attttttaga ctctatttca
    17161 gctgtaaaga atggatatat aaaaaatggt atgtctataa tagatgttgg tacaggtgct
    17221 gggtttcctg gaataccatt gaaaatatgt ttagatagat tagaacttac tttgttagat
    17281 tcgttaaata aaagaataaa ctttttagag gaagttagta gagtattaga gttagataat
    17341 attaaattta tacatggaag agcagaagat tttggtaaaa atgaaaacta tagagaaaaa
    17401 tatgatgttg ctacagcaag agctgttgct ggattaccaa ttcttatgga attttgtgta
    17461 ccctttgtaa aagttggagg atattttatt tgcttaaaag gtccaaatgc taatttagag
    17521 ttagaagaat ctcaaaaggc tattgatgtt ttaggcttag agtatattga aaaaatagat
    17581 gtagaactac cagaaataga tttaaatcat aatatattag tttttaaaaa agtaatggaa
    17641 acaccagtta agtaccctag aaaggctggt aaaccagcta aaaatcctat taagtaatga
    17701 aaaaattatt agcggtaagc ttaattattt ctcaggaaat aattaagctt atcgtaaaaa
    17761 aacaaaaata aaggaattat taaagtagat gcagaaataa atttgtatgt acatatagag
    17821 aacaaaaata atttatatct aaaataagag aggtatgtta tggaagataa aagagttatg
    17881 gagataccta tagaagatgt agttccaaat ccatatcaac caagaaaaat attttcacaa
    17941 gtttcattgg aggaattgag taattctata aaggtatatg gtataattca acctataaca
    18001 gttagagcaa aagatggcaa atatgaacta attgcaggag aaagaagatt aagagcagct
    18061 aaattagcag aacttaaaac cataccagca attataaata atatgaatga tgaatcttct
    18121 gctgttctag cgttattaga aaatttgcaa cgagaagact taaatttcat tgaagaagca
    18181 attggttatg aaaacttaat aaaagaacat gcttttactc aacagcaatt agcagaaaaa
    18241 ttgggcaaaa atcaatctac tgtagcaaat aagcttagaa tattaaagct tccaaacgat
    18301 ataaaaatga agctaataga gaataattta acagaaagac atgctagagc atttcttaaa
    18361 ctaccaagtg aagatttaat gcaaagtgtg ttagacaaag tcataaaaaa tgaattgact
    18421 gttaaaaaaa cagaaaaatt gatacaagat gtattagaag aaactaaaat tcaagaagag
    18481 cctgataaaa aacagaatat aaagggtgct atgagtataa gaatttacat aaatactata
    18541 aagcaggcct ttgatgctat atcaaataca ggaattgatg ctaagtataa tgaaatagat
    18601 aagggagatt atatggaggt tgttgtaaaa atccctaaaa aatagagatt actgcttacc
    18661 agtaatcttt ttttgtaata taagccttgt aatataaatt gatttataga cattatttat
    18721 acttaaaaaa cacctattca acatttaaaa ttaatttaat tcttaaaggg gggaacatca
    18781 tgggcaaagt tatagctgta ttcaatcaaa agggtggagt tgggaaaact acaacaaatg
    18841 taaatttaag tgctagttta ggaactttag gaaagaaaat attagtatta gatttagacc
    18901 cacaaggaaa tacaactagt ggatatggta ttaataaaaa tgaagtagaa aatacaatct
    18961 atgaaattat gttagatggg ttacatatta aagaagcaat aataagtact gaatttgaga
    19021 atgtagatgt agttccatca gctactgaac tatcaggtgc agagatagag cttacatcta
    19081 aaactaatag agagtatatt ttaaaaaatt ccataaaagc agtaatagat gaatatgatt
    19141 atatattttt agattgtcca ccctcactag ggatgcttac aataaattgt ttaactgctg
    19201 tagatagtgt tttaattcca atacaatgtg aatattatgc tttagaaggt gttagtcaat
    19261 taatggagac tataaaatta gtaaaatcaa gactaaatgc tgatatagaa attcaaggtg
    19321 ttgttttaag tatgtttgat ggaagagcta acttatcaat acaagtagtc gaagaagtca
    19381 agaaatactt taaagggagt gtatatacaa ctctaattcc aagaaatgta agacttgcag
    19441 aagctccaag tcatggaaaa cctgtaattt actatgataa aagatgtaga gggtctgtag
    19501 cttatttgga actagcagaa gaatttatag atttagaaga ggaggaatgg tagtatggag
    19561 aataagtcta aaagaagtaa tagattgggt agaggtctta gtgcattgat accagaaatt
    19621 aaaggagaaa ctccagaaaa agaaatagtc aacatagata tagacaaaat atatccaaac
    19681 gaagttcaac ctagaaagca atttgatgaa gaaaagataa aagtattggc tgactctata
    19741 aaaaattatg gtgtattaca gccaatagta gttaaaatgg atgaaaataa taaatacatg
    19801 ataatagctg gagaaagaag atttagagca tcaaaacttg caaataaaaa tcaaatacct
    19861 gctataatta aagatataga tatgaaagat ataatggaga tagctttaat tgaaaactta
    19921 cagagggaag atttaaattc aatagaagag gctctagcat ataagagttt aatagaacat
    19981 tacaatgtaa ctcaggaaga gatatcagaa gctgttggta agagtagacc tcatataact
    20041 aacacactaa gacttttaaa tcttggacaa gatgttatag aaatgataga cagtggtaga
    20101 attacagcag gtcatggtaa ggctttattg agaattgcag ataaagattt gcaacttcaa
    20161 atagcaaaaa aaatagaaga agaagaactt tctgtaagag aagttgaaaa tatagctaaa
    20221 aaaatatctg aaaataaaca agaagaaccg aaaaaatcta aacctaagga tgtatttatc
    20281 ttagatgttg aagataaact tagaaatata tttggaacaa aggtgaatat ctctaaagga
    20341 aagaaaaaag gtaaaataga gatagaatac tataatgatg atgatttaaa tagtattgta
    20401 tcaatgcttc ttgaatagaa caaaaatata aaacagagag gaagattgtt atgatttatt
    20461 tagataatgc tgcaactaca tatccaaaac cagaaagagt gtataatgct gttttagatt
    20521 gtatgaaaaa ctattgtgca aacccaggta gagcaggtca taaacttgct atgagagctg
    20581 ctagggagat atatgacaca agagagaata tagctaagtt gttcaatgta agtaatccta
    20641 tgaatatagt atttacatct aatgcaacag attctttaaa tttagcaata aaaggcgtat
    20701 tacaagaagg agaccatgta ataacaacat ctatggaaca taattcagta ataagaccta
    20761 taaaatcatt ggaaaaaaaa ggtattgaaa atactgtagt aaaatgcgac tatgaggggt
    20821 ttctaaatta tgaggattta gaaaaagcga taaagagtaa tactaaattg attgtaacaa
    20881 cacatgcatc taacgtatgt ggaactttaa tagatataaa aaaagttgga gaaatagcta
    20941 aaaaacataa tatactattt ttagtggatg catcacaaac agcaggtgtt tataatatag
    21001 atgtaaatga atgtaatata gatatgttgg caatgccagg tcataaatgc ttatttgggc
    21061 ctcaaggaac aggtatatta tatgtaagag aagggttaaa cttaaatata ttaaaagaag
    21121 gtggaacagg tagtaaatca gaagaaatag ttcaaccaga gttattccca gataaatatg
    21181 aatcaggaac tcataatact ccaggcatag caggtcttaa ccaaggtata ttatttatat
    21241 ttgaaagagg aatcaacaat ataagacaac atgaagaaga attatgtcaa tatatgatag
    21301 acaaattaga ggaagtccca gatataaaga tatatggacc aaaagatagt aaaaaaagag
    21361 cagcagtaat agctctaaat ataggaaata tggattctgg agaggttact ttcttattag
    21421 acagtgatta taatatagca accagatcag ggatacattg ctctccacta gcacatacca
    21481 ctttagggac tttaaaacaa ggagcagtaa ggtttagtat aggatatttc aacacaaaag
    21541 atgagataga taaagcagtg gaagctttga aaaaaatttc taaaaataaa tagcatgaaa
    21601 tttaagaata tataaaaata aagaaaggag atgtttcaaa ataaaaattt gaatcatctc
    21661 cttttttgta caaaaaagaa aaggggttcc taagaatccc taatctattt gataattgtt
    21721 tcaagaaatt attttaagta atctccttct tcaagaacta tatgaccact ttcataatta
    21781 gaaccatcaa cagtgatttt tatttgttca acattcatat tttttatgta agtacgagca
    21841 actgaatcaa gcattaaagc ttctgcatca ctacctaaat ttgactttgt aaaatcggag
    21901 ctaacatcta atataccagt ttttacacca tcaatgtctt ttgtagtaaa tgaatttaat
    21961 tttgcagatt ctggaacaac tttaagtttt tgaagttctt caaataaagt gttttcatca
    22021 attttactta aatcaacctt gttttcaatt aaatctgtct tatctacatc aaaagagtaa
    22081 atagtagctg attgagtttt agtttcttct tttggagtag gtttagtttt gttattagga
    22141 acctcttttt cagtactatt ttcttctttt ggttgttcaa catttgtatt actcttagtt
    22201 tcttttttag atgtatcatt tccattttga gcattagtac aaccaactgc gaatatagat
    22261 atagataata tacttaacac taacattatt ttcttatttt tcataaaaat ttctcccttc
    22321 ttaaaaacta ttcttatata taattatagt cgttatgagc aaatatatga atacaaaata
    22381 gtaacaaatt taaaaaatga tttatttaaa ttaattaaat tcaaaataaa ctaaatttta
    22441 gtcattttta tgggaaatca tttaatgtac actgaaaata ttgtaaaata aaagtagtgt
    22501 ttttaaaata aatataagtt ataattatat tataaaaaaa tgtcttcaaa aggtgggaat
    22561 agtatggaaa ttttgagctt aggagaaaag attaaaaaat taagaaaaga aaaaaacatg
    22621 acattaaaag aattagctgg agataggata acagcagcac aaataagcca tatagagagg
    22681 gataaatctc atactagtta tgaactgttg gaatatctag gcgataagct agatgtaagt
    22741 gtagaatatt tattagagac caaggaaatg caatcaaaaa agataactga taatttaata
    22801 ctacagagcg aaatttatat aaaagaaaat gagttaaaca aggctgagga ccaaataaaa
    22861 gaagttttag atatatgtaa gagatacgat ttaatggaga actatggcag atgcaatttt
    22921 ttaatggcta atattaatct gaaaaaatct aattataatg atgctataga aaactttgaa
    22981 aaggcattgt acttctttat aaaaaatagt gatagtgaaa atatacttaa gtgttattta
    23041 aacataggga aaatatatat gcaggaagac ttttataaag gtgctataag ccactttaat
    23101 tttgcagagg aaattttgtc agagaataaa tttgaggatg ttaacataca caaggaacta
    23161 tatagtaaga tggcatattg ttacataaaa ctagataatc caaatatgtc attgaaatac
    23221 atagaaaaaa taaatgaaat agataataga aatgatagaa aagaagatgt cgatattttg
    23281 gtattaaaag caaataatat gcttgaaatt gggaaatatg aggaatcaag agattacttt
    23341 aaaaaagcgc taaaaatact tgaaagtgaa gataataaaa ctggattagc aaatgtgtat
    23401 ttaacaattt gtgacgttta cagaaaaata ggtgaaacag atagagtact tgaatattca
    23461 caaaaggtat atgacataaa gaaaaatgat gaagatgaat atatgatgtc aggattattt
    23521 aagataatag aagcatatat agataaagaa gattatgatt tagctagaaa atattgtaag
    23581 atagccttag cttcatcaat aaaaaataaa aataaattca atgaatataa agcattgaga
    23641 ttctatgcta atatgtataa aaatcaaaat gaaataactc ttgcaattga atatttgatt
    23701 aaatgcataa aaatagtatc agatttagga aacaatagaa tattggccaa tttatatata
    23761 gacttaggtc aattatattc aaatatatca aaagaaaaag agttagaata ttatcaaaaa
    23821 ggtgtattta tgtacaaaaa cttagaaata atgtagagta aataaaccta taagggtata
    23881 gaaacaatct ataacaagca agataatatc tatgataaaa tataaattgt atgaatattt
    23941 ttaaggagat gaatcagaca atgagtataa aaatagatgc aagagggctt gcttgtccta
    24001 aaccagttat aaatacaaaa agagaattgg ataaactaga agaaggtgtt gtagttacaa
    24061 tagttgacaa tgaaacagct aaggagaatg tattaaaact tgccaaatct ttaagttgtg
    24121 aagcaaatat tgttgatgaa aaagagaatt ttatatctat agaaattaga aaaggtaaga
    24181 atatactaga taaacaaaat attgtagata ataaagaaag tgaattagac aatacatgta
    24241 tatttatatc ttcagataag atgggacttg ggaatgacga attaggaaaa gtgttaataa
    24301 aaggatttat atatacatta acagaatcta aaccttatcc taagtatgta cttttagtta
    24361 atggaggagt aaaattaagt gctgaaaatg aagaaactat agaaaatcta aaaatacttg
    24421 agaatatggg tgtagaagtt ttatcttgtg gtacttgctt agattattat aatttaaaag
    24481 ataaattaca agtaggtaca gttacaaata tgtatactat agttgagaca ttaaagaatg
    24541 catcaaatac tatttctata taatgtattt ttgtttagta atataaaact aataataaag
    24601 attaactcaa atagatagtc agaaaggatt aagtaatgaa tcaaatgtat atagtgtcat
    24661 ttaattcgac acatcatgcg ataagatcag aaaagttatt tggtgaaaat agtctaaaag
    24721 taatggctct tccaacacca agagaaataa cagcaagttg tggaatatca attaaatttt
    24781 cttttgaaga tatggaaaag ataaaaacta ttttaattga aaataatgta gatataaaag
    24841 gaatttattg tataagtaaa ttagaaaatg gttcaaaaga agtagagaag ttagattagg
    24901 aggctaatta tttatgccaa tggatttaaa aataggtgat attgttgaat taaaaaagca
    24961 acatgcatgt gggtgtaaag agtttgaaat cataagaaca ggaatggata taaagataaa
    25021 atgtactaaa tgttcaaggc taataatgct tgatagagag accttagaaa agagagtaaa
    25081 aaagctaata aaaaagtgat aaaaatttaa cacttaaaaa atgtcaaata ccgtatatta
    25141 atatccaaaa ttttggtata ataagaattg aagaaaacat tttcatgggg gtgctttaat
    25201 tatggcagtt aaatatgcaa aaagaatgca aggtttacaa ggatcagaaa tacgtgagct
    25261 tttaaaactt actcaacaac cacaaataat atcttttgct ggtggaatgc cggctccaga
    25321 gttatttcca gttgaggaaa tgaaaaaagt atcagttgca gtgcttgaag aaaatggaag
    25381 atcagctatg caatatacta caacagaagg atatgaacca ttaagagaaa aaatagcagc
    25441 tagaatgaat gataaaaaca aaactaatgt aaacaaagat gatatattag ttacaagtgg
    25501 ttctcaacaa ggtctagatt ttgcaggaaa agtatttatt gatgagggtg atgtaatttt
    25561 atgtgaaagc ccatcttaca taggagctat aaacgcattt aaatcttacc aacctaaatt
    25621 catagatgtt ccaacagatg ctgatggaat gataatggaa gaattagaaa aaatactaga
    25681 aacaactgat agaataaaaa tgatttacgt aatacctgac ttccaaaatc caacaggaag
    25741 aacttggcca cttgaaagac gtaaaaaatt catggaaata gtaaacaaat ttgaaatacc
    25801 agtaatagaa gataatccat acggagattt aagatttgaa ggagagactt taccatcttt
    25861 aaaatcaatg gatacaaaag gattagtaat attcttagga actttctcta aaatattctg
    25921 tccaggatac agattaggat ggacttgtgc ttctccagaa attttatcta aatttaactt
    25981 tgcaaaacaa ggtgcagact tacaagcatc aactatatct caaatggaag taagcaaatt
    26041 tatggatatg tatgacttag atgcacatgt tgacaaaata aaagcagttt atgttaaacg
    26101 tagagatgta atgcttaaga ctatggaaga agaattccca gaaggattag tatttactca
    26161 tccagaaggt ggattgttta catgggtaga gcttccaagt aatttaaatg ctaaagaatt
    26221 aatgccaaaa tgcttagata agaatgttgc ttatgttgct ggaggaggat tcttccctaa
    26281 tggtggtaga gaaaatactt tcagacttaa ctattcaaat atgccagaag aaaaaataat
    26341 agaaggtata aaaaatatag cagctgtatt aaaagaagct atgggtgtag aagcttaatt
    26401 aaaaataata acttatatat aatacaaggg atggttattg ccatcccttg tttgtttgaa
    26461 aaatatataa tctaccttac actaaacttt gtccaaaatt taaattgaca gaaataagaa
    26521 taaatgataa aatgtgtagg tatgagattt tagtctcatt tctctgctct aagagtgtag
    26581 agccgttaag tccaaaggga ggtgaattat agtgagaaat tatgaattag tttatgtggt
    26641 aaagccaaac tctgatgaag aagtaagaga ggctatacta aacaaagtta aggaagttgt
    26701 tgcaactgac ggagaaatag ttaaagttga tacttgggga actaaaaaat tagcttatcc
    26761 aatagctaag tttacagaag gtttctatgt attagttaac ttcaaatcag cagtagacgt
    26821 tcctaaagag atagacagaa acttaaagat aaatgaaaac gtaataagac acatgatagt
    26881 tgttgcttaa taactggttt tattataaaa aataggtggt gtttttatga atcaagttgt
    26941 attagtggga agattgacta gagaccctga attaagatac atcccaggta caggaacacc
    27001 agtggcatcc tttacaatag ccattgatag agactatgta aaaaaagatg gatctaaaga
    27061 aactgatttt atacctgttg aagtaatggg taaatctgcc gaattttgtg ctaactacat
    27121 aactaaaggt agattagtag cacttcaagg atctataaga gtagacaatt atcagacaca
    27181 atcaggtgaa aagagaactt ttacaaaagt aagtactaga tcagtacaag ctttggatag
    27241 taagaataaa tcggaaaatt catatagaga gagtgctcca gcttttgagc caagctttga
    27301 acctcaaggt ttagacccac aaggtttcca agctatagat gatgacgata taccatttta
    27361 aaaaggaggg aaaaaagtca tgatgaataa aaaaagacgt aagaaaaaaa gagtttgtca
    27421 attttgtgct gacaaaaacg ctaaaataga ttacaaaagt actcaaagat tacaaaagta
    27481 tataactgaa agaggaaaga tattaccaag aagaatatct ggaacttgtg ctaagcatca
    27541 aagagaatta acagttgcta taaaaagagc gagaaatata gcacttttac catatacatt
    27601 agactaaaaa aatgagcctt gtgctcattt tttattatat ggatttatat atctaaatga
    27661 ggcgagctat aaagctcgtt tttttgttta tatacttaaa tatattatta tttatgatat
    27721 aatattatat attaaggagg gttgtaaacg tgaaaataga taaaggttct gagataacaa
    27781 gaaacataaa gataatagaa tggatgaaaa cagaaatatt aatgagtgta agtgatttgt
    27841 ttaatttatt gtttaaaggg gtaaaaccat tagatgaagc aattcaagat actttagcaa
    27901 atataataat gataacatat ctcttagcca agagattagg aatcagtttt agagatgtgg
    27961 attataaagt caaggaaaaa ataaaaattg gtatagatga ggaccatagt gttgaaagat
    28021 ggtatggaga tttatctaat ttaaaaaaac atatggataa tagggagtga tattaaattg
    28081 aataataaaa ttagactatc taaggcaatg tctataataa ttttagctat aataatcgct
    28141 ttagtaatag cctatattcc aatgctagga atatttagta tgttagcagc tgttccatat
    28201 gtaattatag gtgctattac tgatagaaag tattctctta tatctatttt ggttacattt
    28261 tgtgtactga ttctcttttc agacatttct tatgcattaa atatatgtat aatgtattct
    28321 ttaccaggtg tagtaatagg aaagatgcta aagagaagtt ttgaacaaga ggacagtaat
    28381 aaattcgaac cgatatatgg tggaaccata atatttgtac tttcaatgct tgtatatttt
    28441 tttgtattaa agacattttt aaatgtgaat ctattagatg aagtttctaa gatgatatca
    28501 gaaattgtaa atattcaaaa aaatactttt tctgcaaccg agcttaaagt gtttgataaa
    28561 atgaagccag atgagattgt aagttatttt acaaatatgc taccaatgat gttattttta
    28621 caagggttgc tatcagcgtt tgtcacatat tatttgagtg tattttttat aaaaaggatt
    28681 acaaagatga atataagatt tcctaaattt gctgattttt atttaccggg aaatgctata
    28741 ctcacaactt tcttacttta tttattggtc ttatttattc aaattattgg ttcaagacta
    28801 tacacagaat tgataatgac taatttacag ctagttttta atttaatgtt tgtaattcaa
    28861 ggtatagcag tttgcattta ttttttaaag aaatggataa gacagggacc aaataaaatt
    28921 atgttttttt cagggataat tttatgttta tttggattta tgggaatatc ttttgttggt
    28981 atggtagata gtataattga ctttagaaag gtaagaagct ataaatccac ttaggaggat
    29041 agaaatgagt aataaacaaa cctttaaatt aaatatgcca gaaataaatt tatatataat
    29101 tgttattggt atttctagta taattttgct ttattacaac ttgtatgtag ggtgtttatt
    29161 tttctgtatt tttgtatata tggttttcca taattggaga acaactaata tcagacatca
    29221 tgaatggaca gagtatattc aaaatttatc tttagatata gatgaaacaa ctaaaaaagc
    29281 cataataaat cttccaatac cattatgtat tttagaattt gatggaaata tatcctggta
    29341 taatggaaag ttttatgata tgattggtca aaaagattta ctagataaaa atatagaaga
    29401 tatagttaaa aatcttaatt taagaaaagt attaaatgaa aataaagaga tgtacacaga
    29461 aataaactat aaagaaaaag aatatacaat aatttataat gttataaaaa atgatcaaga
    29521 aaaaaatcct aaatatttga tgatattata ctggatagat aagactgaat atttaaaagt
    29581 aaaacaaaat tatgatgatg aaaaaaatgc aatgatgcta atacaagtag atggatatga
    29641 tgaagtatta aaaagtgcag cagaagataa gagagcttta attaacgttg aagtagagaa
    29701 aatactttct gctttagaat taaattcaaa tggtgcattg agaagaacct caaaagacaa
    29761 attcttcttg gtaatgcata aaaaagagtt aaaaaaattg gaagctgaga agttttctat
    29821 tttagataca ataagacata ttgattatgg aaataatctt cctgttacta taagtatagg
    29881 aattggtata gatggagata cattaaatga gaacctaaaa cttgctacag gtgctctcga
    29941 tttagcactt ggtagaggtg gtgaccaggc tgttgtaaaa acaaaagata aatttgtgtt
    30001 ttatggagga aaatctaaag ctgtagaaaa gaagaccaag gtaaaatcta gattaatagg
    30061 tcatgcttta agagaggtta tacaacaaag tgaccaagtt tatataatgg gacataaata
    30121 tcctgatatg gatgctatgg gagcagcagt tggtgtttat gatatatgta aatcatgtaa
    30181 caaaactgca aacatagttt tacaatctgt aaatgaatct atagaaatat ttataaataa
    30241 aataaatgaa aataattact ataaaaaatt gtttattggg aaagaggagg ctattgacaa
    30301 ctgtactaaa aatacattag ttgtagtagt tgatactcat agacctaatt atactgaatg
    30361 tgaagagcta ttaaagctat cagaaaaggt agtagttata gaccatcata gaagaggtgt
    30421 ggagtttata aatgatgcag tgcttttatt ccatgagata tatgtatcct caacgtgtga
    30481 aatggttaca gagttagttc agtatatgga tgaagatgta acaataaata aacttacagc
    30541 tgaaggactt ttagcaggta taagtctgga tactaaaaac tttgcattca aaacaggtgt
    30601 taggactttt gaagctgctt cttaccttag aaaagttgga gcagatacta tagaggtcaa
    30661 gaagttcttc aactctgatg taaaagactt cataataaaa gctgaaataa ttcaaagtac
    30721 taaaataatt aataatagaa tatgtttagc atattcaagt actgagatag atagtataaa
    30781 tgttataata gctcaaactg ctgatgagtt actaaacatc aaagaagtag aagcttcctt
    30841 tgtattaggt gaaaaagatg atactatatt tataagtgcg aggtctttag gacaaataaa
    30901 tgtacatgtg cttatggaaa aattaggagg cgggggacat atagacatag ctggagcaca
    30961 gttaaaaaat gtttctctaa aagaagctta taagatggta aataaaataa tagaggagta
    31021 tttggaggag gaagaataga tgaaagttat attattaaaa gatgtaaagg gaactggtaa
    31081 gaagggagaa atgaaagaag taagtgatgg atatgcaaga aatttcttat ttcctaaaaa
    31141 gatggctgtg caagcagata gtgtagcaat aaaagaatta aatgaaaaga ataaatctaa
    31201 agaaataaaa gcacaaaaag agtatgaaga agcagtttta ctaggtaagc aaatggaaga
    31261 aattaatata gaaatatatt caaaagcagg agaaggtgga agattatttg gttcaataac
    31321 ttctaaagaa attgctgaac aattaaagaa acaaaaagat atagatgtag ataaaagaaa
    31381 aatattatta gatgaaccaa taagagcttt aggttcaact tttgtagaaa taaaaataca
    31441 tcaaaaggta actactaaaa taagagtgga tgtaaaagaa aaacagtaat aattagaggt
    31501 gattaagatg gaagatatga cgagaattcc tcctcatagt gtagaatcag aacaatcaat
    31561 attgggttct atacttttag ataaagatgc tataataacg gttacagaaa ctataaagcc
    31621 tgatgatttc tataaagaag ctcacaaaat aatatatgaa tgtatgataa ctttaagtaa
    31681 taaaggtgag cctatagact tgataacatt aacagaagaa ctaagaaaac aagggcatct
    31741 taatgatatt ggaggaatca gttatattac aagtctttct actatagtac cgacgacttc
    31801 aaatgtaaaa tactatgctg acatagtaaa agaaaagtct gtacttagaa agcttataaa
    31861 agcttctaat gaaataataa atctaggata tagtggagca actaagattg aagatgtttt
    31921 agaacaagct gaaaagagta tatttgatat atcacaggag aaaactagtg acgattttaa
    31981 atctataaac ttagttttga tggatgccta tgatatgata gaaaaattat atactaacaa
    32041 gagcgatgtc actggtataa ctactggttt taaagattta aacaaaaaaa taaatggtct
    32101 acaaaggact gatttaatat taattgcagc tagaccagca atgggtaaaa ctgcattttc
    32161 tttaaacttg gttcaaaatg cagccttaaa aggtgatgct tctgtagctg tatttagttt
    32221 agagatgtct aaggaacaat tggtacaacg tatgttatct tcacagtcta gtgtagaatt
    32281 aaaaaaaata aagactggaa cacttaatga taatgattgg cctagaatta tagatgctat
    32341 ggcagtgtta tcagatgcca aaatacacat agatgatact cctggtataa aaatatcaga
    32401 actaaggtca aagtgtagaa aattaaaaat agaaaaagga ctagacttag tgcttattga
    32461 ttatcttcaa cttatggaag gtgaaggtaa taatgaaagt agacaacaag agatatctaa
    32521 gatatcaaga tcactaaaaa tattagccaa agaattaaac tgtcctgtag tagcactatc
    32581 ccagttatca cgtgcaccag aacaaagagc agaccataga cctatgttat ctgatttaag
    32641 agagtctgga gcaatagaac aggatgcaga tatagttatg ttcttatata gagacgaata
    32701 ttatcatgct gattctgaga gtaagaatat aggagaagta ataatagcaa aaaatagaca
    32761 tggtgaaact ggttctgtgg aacttgtttg gcttggtgaa gttcaaaggt ttggggataa
    32821 gttgagagac ctataatata acgaatatcc acagaacgtc ctttctttct acaggaaaaa
    32881 tgtggataca tgtcactaaa tatgtctaaa cattgaaatt tagctaaaaa attatccaca
    32941 gtatccacag atagcctgtt gataagtgtg aataagtaga taaagcaagc ttttggcttg
    33001 ctttatctgt gtataaatat taattattag gaggaataat atggaaaaaa taagcttaaa
    33061 atatatatat cccaatataa ttaaagtact tgatgaaata aacttattta gagtaattga
    33121 taataattta agagaatcta ttgttgttta tgctaataat gtggataatc agtatcatat
    33181 aaacatgact aatacaaact ttggaaacat aattaacata tgtaaattag aaaaactttt
    33241 agatgtggat aagtttatgg aaaaagttat aaaatcagaa aaagaaataa tagagaaaga
    33301 agagttttca aaaatagaag aatatatgtt aaatattgga gaatactaaa aaagttgaaa
    33361 aaaatacgaa tgtttgatag aataagtaag tagagtgttc gagagtatat aaaatataga
    33421 ggtgaattac atgaaaacag ttgcaattgt tggttctcaa tggggagatg aaggtaaagg
    33481 taaagttata gattatcttg ctactcaagc agatgtagta gtaagaggac aaggtggaaa
    33541 taatgcagga catacattag ttgtggaagg taaaaaatac gcattacatc taattccatc
    33601 tggagtttta aatccaaata cagtgaatat aatcggaaat ggaatagtgt ttgaccctaa
    33661 aggattttta gaagaattgg aaatgtttaa aatggataat ataagtacag aaaatataaa
    33721 aataagtaat agagcacatg tcatattccc atatcataaa gaactagatg tattgtcaga
    33781 agaagccaga ggcgatttaa agataggtac aacaaaaaaa ggtattggac cttgttatat
    33841 ggataaaaca gagagatctg gtattagaat atgtgactta atggataaag ataaattcgc
    33901 tataaaatta aaagctcaaa tagatgctaa aaatgaaata gttaaaaata tatatggaaa
    33961 agaagaatta tttgattttg aaactatata taatgaatat ttaggatacg cggaacaaat
    34021 aagaaaatat gtggcagata catcagttat tgtatatgat gcaataagag caggtaaaaa
    34081 agtattgttt gaaggagcgc aagggacttt attagattta gatttaggaa cttatccatt
    34141 tgttacatct tctcatccaa catctggtgg atttgcaatt ggtgcaggta taggaccaaa
    34201 tatgataaaa gatgttgttg gtatagtaaa agcatatact acaagagttg gagaaggacc
    34261 atttgttaca gagcaaataa atgaaactgg agataaaata agagaacaag gtcatgaatt
    34321 tggggtaact actggtagac caagaagatg tggatggttt gatgcagtta tagttaagta
    34381 tgcagctaga gttaatggat tgactagtat ttcatttatg ctacttgatg tattaactgg
    34441 atttgataag ataaaagtct gtacatctta caaaatggga gataagataa taacagattt
    34501 cccagcttca ttagatgatt tagcaaaatg tgaacctgta tatgaagaat tagatggatg
    34561 gaatgaagat ataactcaaa tagataactt tgatgacctt ccagaaaatg caaagaaata
    34621 tgtagccaaa atagaagaac tagttggagt aagtgtagat atggtttctg ttggtccaaa
    34681 tagagctcaa accataataa gaagaaatat atttgcttaa tattttatca attagataat
    34741 ttaaattgta aatagttgaa gaagagcatc ttaagataat gtttataatt attagagatg
    34801 ctcttttatg ttcgacttaa atttattttt tattaagtta aaattactga cacacattct
    34861 aactgaaatt aaagttgaat ttgtgtagag atgtataggt ttttaaaaag aattttgttg
    34921 aaaataaata gctaaagatg attttaatta tatgctaaaa aaaataaaaa aaactattga
    34981 ctaaaaattg tatagaagct ataatataac ttgtgagtga aagaaacgac gaaaaaagtc
    35041 gaaaaaatgt ggtctattag ctcagtcggt agagcacctg acttttaatc agggtgtccc
    35101 gcgttcgagt cgcggataga tcaccaaaat atggctcgtt ggtcaagagg ttaagacacc
    35161 gccctttcac ggcggtaaca ggggttcaat tcccctacga gtcaccagta tgggactata
    35221 gctcagctgg gagagcacct gccttacaag cagggggtca caggttcgag ccctgttagt
    35281 cccaccatat ttttaattag agataaaaac tagattatat gctggtgtgg ctcaacggta
    35341 gagcagctga cttgtaatca gcaggttgta ggttcgattc ctatcaccag ctccacaaag
    35401 tacataaatt taaattaata taattttcta tgttacagga tacttttaag tatctttttt
    35461 taatttcatt ttttattgta ttaaaaatga aatttacata taacaaaata ttgaattact
    35521 tgtaatgtga tagttttgaa tctaaaattt agaaacattc tagaatagta ttatgtaatt
    35581 agcaaattta atttatagta ccaataaata tagattgcta agaatataaa tgatttgtta
    35641 ggttaagata cattagatat gtaaatagaa aaataaaaag tatggtttaa tttatgtggt
    35701 ataagtttta aacagagaag caagtttgct cttacctctc tatttaaaaa taaaaaaatg
    35761 ttttttaagg gctattttat ttgaatcttt cccatctcaa atcatctcca gtaaattcta
    35821 aaatcctaaa cttatccaag attctagaga gagttctttg agtataagta tttcctatct
    35881 gagatggtgt taagttggtt gaaataatga tttttttacc agcaacaagt cttgtattta
    35941 caatattaaa gatttctcca ctagtaaaag aattattaag ttcagtacca aggtcatcta
    36001 ttattaataa atcgcaatca aacaagtttt tataattatc ttcacttatc tgattatttg
    36061 agtctctcct aaatttataa tcttcaagaa tatctaagat tctaaaagaa gtttgataga
    36121 ttacaacatt gcctttatct aacaattcct ttgcaataca attgcacata tatgtttttc
    36181 ctagtccagt agaaccataa aacagtaaat tttcactatt atctttctta aaatcatgaa
    36241 caaaattttc gcagatactt aaattgttta gcatattttc tctaggagat atttccccat
    36301 cagaactttt cttaggagaa aatatattca agttaaaatt cgaaaaattt tcctgactta
    36361 aaatcctatc taaattagac attttataag cttcatttac aatttcttgt ttaaggcaag
    36421 aacatttttc accattaggt aaaaatcctt tatctttaca agaaacacat tgaaacttta
    36481 tttccaaata atcaagaggt atgttatttt cagttaaaag tttttctttt tttaatttta
    36541 aagactctat attctcttta gtcttcttta caatttcatc tttagattta ggatttaaaa
    36601 gtactatttt agctaatctg agacctattt tagaaatttc atcatctaaa gattttattt
    36661 cagggattct attatatacc tcattttttc tatgttctaa taagagttca ttatcatctc
    36721 ttctcttagc atatttagca agtatttttc ttattttatc ctcattcatt taatcaccta
    36781 cttaaacttt tctttttgac tttttttaat aatctcatcg agctcatcag atgtatattg
    36841 agtgaatgtt tcgttgaaat tatgaaactt agttttcttg taagtattgt tgttttgagt
    36901 agtattaatc tgcttcttat taatattctc cttaactata cgttcttcct ctttttgctt
    36961 taaatcattt aaatttttta tatttttttc attccaattt ttaattatac catttatata
    37021 agatatactt gggtttgaaa tgttcttaga cttggaacat gcttttatta tcaaatctat
    37081 atccatatta aatttatcaa accaagtgtc cataagttct ttttctgact ttgatggttg
    37141 tcttgaaaat cccaattcat tgaaaatcgt tttatataaa atatatcttt cacttcttac
    37201 caaaaagctt tcttcaacat cttttggtgt atataaattt gaatcatacc aatttctaat
    37261 aataccttca atatatttaa ctggctttga tgtaccagtt ttatctttga cgtattcata
    37321 tgcgcaaagt atcatatcag ggctcatatt gtatttattc attatatcca taatactcat
    37381 cttttcactt gggtcgagat atcgcccaac tattttattt atagagttaa acatttttct
    37441 aatgcttgga ttttcagaag tagatacaac cttatctgta ttagatttta cagatgaatt
    37501 attgcttaat atattctcaa tatagaaatt ttttaaatct aaaaattcta ttgaataatt
    37561 aaaattatca tattctccat tgtcatgtat ttttataatc ttcttttcct cccagaattt
    37621 ccaagcagat aacacatcag acaatggtat atttaggttt ttggctattg aactattatc
    37681 gaacttagga ttagaagtaa tgtcgcatgc ctgtctatat cctaaaagat aaacttttac
    37741 gtataagccg tccgccattg gcataaatat atctataaag atattgggaa tagtgatttc
    37801 acctaaatct atttcattgc tttctttaaa aaacatttta tcacctcaga ttaaattata
    37861 tcatttttta tattatattt gtctgtaaac aaataaaagg tcacaagatt agcatgtaaa
    37921 tattttctaa aagtattcat tttgaaaagg aggagatatt ttggttataa aattcaacca
    37981 aaaagtaaga agaacattat cagtagcgat tctatttatg gtattaataa taggttgctt
    38041 tatagaaaag gatgacagtt tagaaacata caaagaaaaa actaattcaa aaataaatca
    38101 atataatatg gatgttatat ttgatgatga gactaagagg ttaatgtgca atcagaatat
    38161 tgagtacata aataatacaa aatcaaacat agataaaata tatttacata tatatccaaa
    38221 tgctttttcc aaaaaagatt ttgctccttt tgaaaaaagt gaaatgacaa gggcctatcc
    38281 taatggattt aacgaaggat atatagatat aaagaatata ttaaataaca atagtaaaat
    38341 ggagtataaa attaaagggg acaaaaatga catattagaa atagaattgg ggaaagagtt
    38401 aaaaccaaat gaaagaatgt cattagacat aaaatataac gtaaaaatac caaactcggt
    38461 tggaagattt ggatatggag aaaatactat aaatgttact aattggtttc ctatagtttg
    38521 tgtaaatgat gaaaggggat ggaatttaaa gagttatgaa actattggtg acccatttta
    38581 tagtgaaact agcgattttc atgtgaaact attgattcca aataaatata aaattgctca
    38641 tacaggaaaa cttgttaatg ataaacatga taataagaaa atgctatatg aaatagaagc
    38701 taaaaacgtt agagattttg catttatatt aagtagtaaa tttgatgtga ataaagtaga
    38761 ttcaaagggc gtagctatca atacatataa tttaaataaa aagctttcta aaaaagcaac
    38821 tgaagttgca aaagattcaa taaatatatt tagtgaatta tttgggaagt atccttacaa
    38881 agaattttct gttgtagcaa gtgacttttt tataggggga atggaatatc ctatgcttgt
    38941 tatgatagac caaagtttat ataatgaaaa aaatgaattt ttactagaat atgttattgc
    39001 tcatgagaca gcacatcaat ggtggtattc tgccgttgga aatgatgaaa taagtgaacc
    39061 ttggttagat gaagcactta ctgaatattc cacaatagtt tattttgaac aaaagtatgg
    39121 taaagatatg gcaaataagc ttataaaaac tatggagata cagacaaaaa gttatttgag
    39181 cgaaaatata tttaggccag cgaatgaata taaaaattct actgaataca gtttaaatgt
    39241 ttatacaaaa ggcgcaatag cgtttaatga aataagaaaa gaagttggag ataaagtatt
    39301 ctttgagact ttaaatgaat actataataa atataaacat aaaaatgcta atggaagagc
    39361 ttttgtggaa ctatggaata gtaaaggggt agatataaat aagattataa gtgaatgtaa
    39421 gtaaatttct tatagagtat taaattttat ttaatactct atttttttgg tataatataa
    39481 tttataaggc agaaaaagag gtgaaggaat gctaaagtat tgttcagtag ggagtggtag
    39541 tagtgggaat tgccactata taggatataa aaatactaat atactagtag atgcaggatt
    39601 aagtggcaag agaataacta caggtctcaa agacattggt atagatgcag ataaattaaa
    39661 aggaatattt ataacacatg agcatgttga tcatataaaa ggagcaggta tactatcaag
    39721 aaagtttgat ataccagtat ttgctaatat aaagacatgg tgttctatga aggacaaatt
    39781 aggtgatatc aaagataaaa atatgaaagt atttgaaaat gataaaacct attcattagg
    39841 tgatatcata gtgagacctt tttcaataga acatgatgcg tctgacccag ttggatataa
    39901 tttttataca gagaatgatg aaaaaatgtc tatagctact gatataggat gcataactca
    39961 aaatataaaa aaacatctat ataaatctaa gttagtagta ttggaatcaa attatgaccc
    40021 taacatgctt atgatgggtt catatacata tgctttgaag aaaagagtaa tgtcaaatgc
    40081 tggtcattta tcaaatgaag atgcagctaa tttctgtgtt gaacttatta atgaaggcac
    40141 agaatctata ttattagcac acttaagtaa agaaaataat tttcctgagt tagcatatga
    40201 gacctcaaaa ggagtattag catcaaatga tatagtagtt ggacaagatg taaaactaga
    40261 tgtactatca cgaaatgatg tttctaatgt ttatgagatg aagtagaggt gaataacttt
    40321 gctaaagaga aatttcgaaa acgttgaaga taataaaatt ttatactcta taaagcaggg
    40381 aatgatttta gcaatacctg ctattatgac tggttcaact gctcttgtaa ttttgaatct
    40441 tccaataaag gtttatcaag aatatttatc aagcttgttt aatggagagg ttacaaatat
    40501 tcttaacttt ataaatgatt ctactttagg aattatttca ttaataatat tattaacaat
    40561 aagctatagt tatgggaaaa tatatgggag caagtataca gtacttgttc ctatagttgc
    40621 aatgtgctct tttttagtat tttcacatgg taatgagctt gattcctata tagaaatatt
    40681 taagacaaag tggttattta catctataat tgtttctatg acatcttctg tattatttgt
    40741 aagacttaca gaatcttttg ttacaagtgt aaaatttcat acagaagggg ctgatgcaga
    40801 ctttaatatg gtagtttcag ctatagttcc tttcataata gtagtatttt tattctctat
    40861 ttttagagta ataatgataa gtctaattgg cagttcaaac tttcaagata tattttataa
    40921 tttattttca aatgtgttta atagaatggg aacaaatctt atgagtgctc tgttgtttat
    40981 ttttttaatg cactttatgt ggttttttgg gattcatgga ggtaatgtat tagatactgt
    41041 tgcaaaaaat ttatttgaaa atagcatggc aattaacata aatttagtta ataataatca
    41101 gttaccaaca gaaattttta ctaaaacttt ttttgataca atggttttat tggggggatg
    41161 tggcttttta ctatgtctag taattgcaat ttttcttagt gagaagcgta taaatgtaag
    41221 aaagttagct aaaattgctt ctataccagc tgtttttaat ataaatgaga tgctagtttt
    41281 tggaatacca attgttttta attatattat gtttgtacct tttgtaatta ctcctataat
    41341 tttaaccatt acaagttatg tggctatgtc tacaggcatt gtaccctgta cagtttcagc
    41401 tgtagaatgg acatcaccga tatttttaag tggatatata tctacaggtt caataagggg
    41461 tagtttgttg caattattta atctatgtgt tggtgttatg atttatattc catttataaa
    41521 gatatcgcaa aatagatata catatatgtt taaaaataat atagaaaatt taacaaagct
    41581 agtaaaaagg tatgaattaa caggggaaca gcctaatttt ctaaaccata atggtaaaat
    41641 aggctcaatt tcaaaaatgt tggcatcaga cttgaagttt gctatgaaaa gaggagaaat
    41701 agagattttt tatcaaccac aagtcaatta taatggagat gttgtagggg cagagggatt
    41761 acttagatgg aaacattcga taggagggtt catttatcca ccactagtta taattttggc
    41821 taaagaagaa ggttttctag atgaattagg agagtatatc ataaataaag catgtgttga
    41881 tttaaagaaa agtgaaaagt tattgagaaa ttctgttaag ttttccgtaa atatatctcc
    41941 agaccaactt gatgaaccta agttaccaga acaaataaaa aatatcatta gtagtaatga
    42001 tataaatcct aatatgctag gtatagaaat tacagagcag gttgcattat caggttcaca
    42061 aataattatt gaacgtatca atgcgataca taacttggga ataaagctaa ttatggatga
    42121 ttttgggatg ggacatagtt ctcttatata tcttcaaaat aataattttg atatagttaa
    42181 attggatggt tcattagtta aagagatact tacaaataaa agaagtagtg aaattataat
    42241 gactattgta aatctttcaa aaaatttaga ttttgatata gttgttgagt atgttgaaaa
    42301 tatggaccaa agagataagt tatataggct aggatgtgaa atatatcaag gatattatta
    42361 tagtaaagct ataccttttg aagagtttat agagtatgca aatcaagaaa gagagatata
    42421 attttataag agcttgtaaa ttctagtaag gtatagattt gtaataaaat taagtttagg
    42481 agaaatatat gaatataagt atagtagtag taggtaaaat taaagaaaaa tatttaaaat
    42541 taggtattga tgagtttaaa aaaagacttt ctaagtattg taagttagaa attatagagt
    42601 tggatgatga aaaagctcca gagaatttga gttctaagga aatggaaatt ataaaagata
    42661 aagaaggaaa gaaaatttta gggaaagtaa agcataatag ctatgttata gccttggcta
    42721 ttgatggaaa gagcttgtcc tctgaagaac ttgctaagac catgagtgat ttagcagtta
    42781 gaggtaatag tagtctatgt tttataatag gaggttctct agggctttct gatgaggtct
    42841 taggaagagc tgattatagg ttatcttttt ctaggatgac ttttcctcat cagatgatga
    42901 gattgatatt gctagaacag atttataggg cttataggat aaataatgga gaaccgtatc
    42961 ataaataaat gatacggttt ttgttagttt gccacttaat aatattaaaa taacaaaaaa
    43021 gtattgaaag tatattgtat ttaaggtaga attaaataaa atattataac attataatgt
    43081 ttagagggtt gttaataaaa atttggagga aaatatatga taaaaataat gttgtcctgt
    43141 tcagcaggaa tgtctacaag tttattagtt actaaaatgg aaattgcagc taaagaaaac
    43201 ggtatagagt cgcaaatttg ggcaatacca gaatcaacta tacaaaatga aatagaaaaa
    43261 tgtgacgtat tattactagg accacaagtt agatacgtat tacctaaggc tcaggaaatt
    43321 gctaagccat ataatatacc agtagaagtt ataaatatga tgcactatgg aacagtcaat
    43381 ggagaagctg ttttaaatag agctatagaa cttaacaaca ctaagtaata taaaatataa
    43441 tgtattttta atatatcaat tgggagaatc aaagaatgga tgaaaagatt atagaaatat
    43501 cttttaatat aataggttat gcaggtaaag gtaaaggatt agcctttgag gcaataaaag
    43561 aagccaagaa tggaaatata gaaaaagcaa aagatttatt aaaagagtct aaggaagtta
    43621 taaataaggc acataaatat caaacagaat taatacaaaa cgaagctagt ggaaacaaga
    43681 ctgaaataag tgttatactt atacatgcac aggaccactt aatgaatggt atgaattttc
    43741 aacagttggc tgaggaaatt gttgatttac atttaaagct acaagataaa taaaaattaa
    43801 agaaaataaa ttaagagagt tcttaaaata tacattatgt gtagcacact aattgttaga
    43861 ttattgttta atatttggtg agcaatacat taagtttatt ttgaggactc tttttgtatt
    43921 tagtttaaag ttaatagata tttattatta ataatataag attatatgtt ataatattat
    43981 aacatataat ttaagaggtg aatcatgtta agaaatcaac cattatatat acaagtcgta
    44041 gatatattaa agcaaaaaat attaaataac gaatataaaa ttgatgaaaa tattccaaca
    44101 gaaagggaat tagagcaaga gtttggtgtt agtaaaatta ctattagaaa agctattgag
    44161 atattagaat cagaagggta tgtagagaaa aaaagtggaa aagggacaaa agtaataagt
    44221 aatagccttt ttaataagct atcaaaagcg gagtcttttt caagcatact tttatctaag
    44281 ggacataagt tatctaaaga gactatttca attgaaaaaa taataaacga tacaagttca
    44341 caagtattta gtatatttgg agaaaaatgt agcaagatta ctagaatgta ttacttggat
    44401 agtaagccat atatattttt tactcattat ataccttttg atgaaaatct aactgttgaa
    44461 agttttaata atgaaagttc tttatatatg tatttatata ggagaaatat aaatataact
    44521 agatttgaag atgaattttt tatagaagaa tcagaactag aggtatcaaa ggctctaaat
    44581 ctagataaag acatattatt aggaagaaaa agaaagacct atgatgagag tgacaatata
    44641 gttgagttat catttgcaag atataataca gaagtacaaa attatgtaat taaatatgaa
    44701 atataataag aatcctgagt ttattaaaat gataatatac tcaggatttt tatttagttg
    44761 taaaaaataa tattataaca ttataatgtt tacaaacaaa cattataatg ttataatatt
    44821 tttaataaat aagtgatatt atcaattaca taaaaattag tttgtattat gaaatcgtta
    44881 ttgcattata aataaaggat aatatcaata atacaggggg ataactatgg aaaaattcgc
    44941 aagtttttta gagcgcaaga ttatgcctgt agcagcgaag ataagtaatc agagacatat
    45001 gagagctgta agagcaggta taatagcaac attaccacta acaatagtgg ggtcgttttt
    45061 cacaatatta ttaaacatac ctattgatgc ttatatggat tttatagctc catttaaagc
    45121 aaccttagac ataccattta gatatactgt aggattttta tctctatatg ctacttttgg
    45181 gatagcatca tcacttgcta aaagctatga tttagacacg acaggtgttg gtatgcttgc
    45241 agttatggca tttttagtct ctacaatagt tccaactcaa gtagttgaat cagtaggtgg
    45301 cgttatagaa gctggtagat ggatggatat ggcaaaactt aattcgccat cattatttgg
    45361 agctattgta acatcattaa ttacagttga aatatatcgt tttatgaagg aaaagaatat
    45421 tacaattaag atgccagcag gagttccagt agaggtttca aattcattta ctgcattatt
    45481 accaacagca gtaatcttaa tgttattctg ggtaataaga catattctag ggtttgatat
    45541 aagtgctgca ctaagtgcta tgttatcacc tttaaaagga ttcttatcag gaaatagttt
    45601 aggtggtggg ttactgacag tattcctaat aactttattt tgggtacttg gaatacatgg
    45661 tccagctata atgggaccag taataagacc tatatgggat atgtctatag cagaaaatat
    45721 aactgcattt acagaaggag tatcatctca tgcaatgcca aacatattta cagaacaatt
    45781 tttacagtgg tatgtatgga taggtggagc aggagctact cttgcattag ttgtaatgtt
    45841 tatgttttct aagtcgcaat atctaaagag tttaggtaga ttagggtttt taccagggtt
    45901 attcaacata aacgaaccta taatatttgg agcgccaata gttatgaatc caatactagg
    45961 gattccattt ataattggac ctataataat gacaattcta tcatatgttc ttactattac
    46021 agggattata cctatgatgg tagcaagatt accgtttgca atgccagcac cgatagcagc
    46081 agtaatgagt actaactgga gtatactagc aggtatctta gtattaatca actttgtgat
    46141 atcatttgct gtttattatc catttttcaa agtttttgaa aagcaacaat tacaaagaga
    46201 acaagaagaa atgagcgaaa atttataaaa atacatatac atagaagttt tttactatat
    46261 aaaatataga aaaaagctta taagttatta aaggtgaagg taggaggaat gaccttcgcc
    46321 tttaatttta tatattttaa ataatgtgat ttgaggagga gaaatatgga aagacgatta
    46381 gggatatcag tatatccaga gcatagtaaa atggaagatg ataaggcata tataaaaatg
    46441 gcttcaaaat acggattcaa aagaatattt atgtgtatgc tttcaattac aagacctaag
    46501 gaagaagtta aaaaacattt taaagagatt ataacctatg caagagagtt aggatttgaa
    46561 gtaatccttg atatagctcc aaacatattt gatatattag aaatatctta tgatgattta
    46621 acattcttta gtgagttaaa tgctagtgga ataagacttg actatggata tgatggaagt
    46681 aaagaagcta tgatttcttt caatccatac gatttaaaag tggaactaaa tatgagtaat
    46741 gatgtagcat acttggataa tataattact tacaagccaa atgtaaacaa tatatatggc
    46801 tgtcacaatt tttatcctca aagaggtaca ggcttgccat atgatttttt tgtaagatgt
    46861 tcaaatagat ttaaaaagta tggaataaga actgctgctt ttgttaattc aaaatatgca
    46921 acaatcggac cttggaatat aaatgatgga ttgtgtacgc ttgaaatgca tagaaactta
    46981 gatattgcta catcaactaa acacttattt gcaacaggat tgattgatga tgtaattgta
    47041 ggaaatgcat atgcaagtga agatgaatta aaaagaatgt ctgaagtaga tagataccaa
    47101 gttgtattta atgtagtagc taaggaaaaa acttctgaaa tagaaaaaac aataatggta
    47161 aatgagcaac actttagaag aggggatata actaatcagg tagcaaggtc tacagaagta
    47221 agaaaaaaat atgctaaaga aaatttccct aagcatgata atactaacat ctttaaaaga
    47281 ggagatgtag taattggtaa tgatgacttt ggaaagtata aaggggaatt acagttaata
    47341 cttcaagatt gtacagatga aagaaaaaat aaggtaggta gaataaaaga agacgaaata
    47401 atacttttag actttattga accttggagt aaatttaaga ttattttaga gggagagtaa
    47461 agtgaagata gacatattaa taaaaggtgg aaaaacagta gataaaagta taatagatat
    47521 agctatatta gacgataaaa taatagaagt aaaaaatcag atagatgatt ctaagtatca
    47581 ggcaaaccaa attataaatc tggatggaga gaaatttatt agtgcgggtt ggatagatat
    47641 acatacgcac tgctacgaaa gactagacct ttataaggat tatccagatg aggttggaat
    47701 aaaaagtgga gttactactg tagtagatgc aggaacaaca ggagctttag atataggtaa
    47761 attttatgaa gatactaaaa agtataaaac taatgtatat gctttaataa atattgctaa
    47821 gcagggaata acatctcaag atgaactttc aaatatgatg aatattgatg aagatgaact
    47881 taaaaatgcg gttaaaaaat acaaagattt tgttgttgga atcaaagcta gaatgagtaa
    47941 aagtgtcgta atttcaaacg atattaaacc tttaaaagta gctaaaagaa taaaaaacga
    48001 acttaactta cccatgatgg ttcactttgg gagttctcct ccaatgatag aggatatatt
    48061 cgactatatg gaaaaaggag atatattaac tcatatatat aatggaaagc caaatggaat
    48121 acttagaggc aatgaggtta aaaaggaaat tatagaagct agagaaagag gtataatcct
    48181 tgatgtaggt catggaactg aaagttttag tatggatgtt gcaatgaaat ctaaggatgc
    48241 agagattttt ccagatacta taagtacaga tatatatatt aaaaacagaa taaatggtcc
    48301 agtatataat ctatctacaa caatggaaaa gtttatctat atgggttact cacttgaaga
    48361 tataataaat aaagttacta agaatgcagc aaaagcaata agtttaaaaa ataaaggttt
    48421 aataaaagaa ggatatgatg ctgatttaac tatatttgat gtggttgatg aagaaaaaga
    48481 acttcaagat tctttgaata agtcagtaat tacaagtata tctataaaac caagagcagt
    48541 agtagtcaat ggagaatatt taaatatagg taagtctata cgagagaatg agtagaaggg
    48601 agaaactatt gtatgagtag catatttgaa aaatactctg taagacgtgt tataaatgct
    48661 tcaggtaaga tgactatact aggagtatca acagtagata aagaagttat tgaagcaata
    48721 ggtgaagggt gtaaaaattt ctttataata gaagaacttg taaataaaac aggtgaatat
    48781 atagcaaatt tattagggtg tgaaagtgca ctaatagttt ctagtgcatc tgctgggatt
    48841 gcacagagtg tgggggcaat gatagcaaaa gatgatatga gtctagttta taatgtaaat
    48901 aatcctaaga agcaagttaa aagagaaata ataattccaa aggggcacaa tgttgattat
    48961 ggagttccag tagaagtaat gataagactt ggaggaggag aagttgttga ggctggttat
    49021 gctaatatgt gtaacaagga acatataata agagagataa atgaaaatac agcagcaatt
    49081 atgtacatta aatctcatca ttgtgttcaa aagagtatgt taagtgtgtt agatgcagta
    49141 caagttgcta aagaatataa tttacctttg atagtagatg cagcggcaga agaagattta
    49201 aatgtatact atgaattagg agcagattta gtgatataca gtggtgctaa ggctatagag
    49261 ggtcctagct cagggttagt aataggtaag aaaaaataca tagataatgt aaagcttcaa
    49321 tcaaagggta taggaagagc tatgaaaata ggaaaagaaa atattgtagg tctaacatgt
    49381 gcaattgaga gatatataag taatgataaa gtaactttaa aagaaatgga ggacaagcta
    49441 aatccattta tagataagat aaatactcta aagggagtga gtgctagtat aactagagat
    49501 tcagcaggta gagagatact tcgtggagaa attgacttta atgaagatat aataaataag
    49561 tctacacaac aaattataaa tgaacttaga ggtggagaga tagccatata cactagggac
    49621 tataaggcta atgaaggtaa gattgagatt gatataagga gtgtaactgg aggtgattta
    49681 gatataattt ataataaaat aaaagagata atagagaagt agagagtgga ataagtgaca
    49741 ttataatgat ttaataaatt gaattttacc aatggagggg ataaatatga attgtacacc
    49801 acaatattat aaaaatagag tttgtttgaa tgttcttgca ggaagtataa aaaatgctaa
    49861 agaagtatat gatgcttgtg atgggcatat actagtagga ttattatcca agaattatag
    49921 cactgtagaa gtagctattg aagatataaa aaaatactca aaggaaatag ataatgcaat
    49981 atctgtgggc cttggagctg gagacccaaa tcagtcaaat atggtatctc agatttctaa
    50041 agaacttcag ccacaacata taaatcaagt gtttacaggt gtagctacat ctagagcatt
    50101 gcttggtcaa gatgatagta taataaatgg attagtttct ccaactggta aaccaggatt
    50161 tgtaaaaata tctacaggtc cattaagttc aaaagaaaaa gatggtatag ttcatataga
    50221 aacagccata gccatgatga aagatatggg aggaagctca attaaatttt tcccaatggg
    50281 agggcttaaa acaaaggatg aatatataga agttgcaaaa gcttgtgcaa agcataattt
    50341 ttatctagaa ccaacaggag gtatagactt agataacttt aaagagatag ttcaaattgc
    50401 acttgatgct ggtgtagaaa aagttatacc tcatgtttat acatcaataa ttgataaaga
    50461 aacaggtgat acaaaagtgg aagatataga agtactttat aaaataatga aagaattatt
    50521 gggataaatt tatgaatata ttagcttttg gtgaaataat gatgagactt tcagtacctg
    50581 gttataagtt tttaacacaa accaatgaac taaattatat aattacaggt acagggttaa
    50641 atatattatc tgggcttaaa aactttggat acaataccta catgttgaca aagttgccta
    50701 ataataatgt aggtaaagca tcaagtgcaa atgtaagaaa gcttggagta aaagatgact
    50761 ttatcacata tggaggaaat catataggag tttactttct tgaaagtgga tatggagaaa
    50821 gaccctcaga agtgacttat ttaaacaggt taaatagttc attctgcgag agtaaattac
    50881 aagattatga tattgataaa tgtctagaag gaatggatgt agtccatatt tgtggtattg
    50941 ctatgcaact tacagattca gttaaagaga ttgctttaga actagctaga aagtcttatg
    51001 aaaaggggat aaaagtattc tttgacttta attttagaca atctctaaat ccagaaaggt
    51061 cttatgatga tttagtaaat gattataaga aaatacttcc atattgtcat ggactgttcg
    51121 ctagttgcag agatataaat aaaatactta atatagataa tctagatata aaatctagtt
    51181 cagaattgat taaaaaagag tttgatatag atattgtagc tggaacgatg agaagatttg
    51241 atgatatgaa taatagattt ataaagggat atatatcaaa tgatgatggt tattttgaaa
    51301 gcaaggagta taagttagaa atatatgaca gagttggtgc tggggatgga tatgtcgcag
    51361 gaattatata ctcatatttg gaaaatatga gtaatgaaga tatagttgag tttggggttg
    51421 ctagttcagt gttagctcat acaacttatg gggataattc tttagtaagc acaagtcatg
    51481 tacttagatt gatgaacaat gaaaaaatag atatattgag ataacacctt tatatagaat
    51541 aaaattttta tttttttcag agtcaccttt gacttgctta tatttgttaa acatagttct
    51601 ttttagtgat tatgaatatt aaattaaaag ttaaataata aaatagtaga aaatagcatt
    51661 aaccatagaa aaaatggtta atgctatttt tatagtcaag attaattaat tttatattat
    51721 agaaatgaac ttttctaatg ctggatttgt atttttttta ttccatacaa ctacaacttc
    51781 aattctattt tctacttctt ttataggtac tattgcaata tcacgttcta tcattgtatc
    51841 tgtaacgcaa aatgaaggta aaatggatat acccatgtta gaagctacca atgattcaat
    51901 agtattaaga ttatttgaac gttgtaccac attaggtaca aaccccgctt cacgatatct
    51961 attctgtatc attgactttt gtccatactc atcccctgtt tcctttacaa aaataaattt
    52021 atcatgttgt aattcaatta aatcgataga atcctcattg taaagtggat gaccaggagg
    52081 taaaagagct accaaagaat atctttctac agttttatgt tcaaaatctt ttaatggttg
    52141 attttctgga ttaataacaa aagcaaggtc catagaatta ttttttacta agtttaaaag
    52201 ttcggttatc tcattagtac aaatatccat agatatatta gggtatgtat tagaaaattc
    52261 ttttaaatat tgttgaagtt tgtttttttc gtatcctatt aaggttccaa tgctcaaagt
    52321 tccaaaaaaa ccatgtgcaa attgttgtgt ttttgcaata gcatcattga tattttttat
    52381 tatagtgcgt gcctcagata aaaaaacttt tccagctggt gttagttcaa catggcgttt
    52441 acttcttgta aatagagtta catttatttg ttcttctaaa gattttattt gctgtgtaac
    52501 agcagtttgg gaaatataaa atttttctgc tgttttggta aaacttagat tttctgctac
    52561 agatataaaa tattctaatt gtttagtatt caaataatca cccctattta aacttgattg
    52621 tttgtaaacc ttatttatat ataattaaaa cttattatag tataagaaaa tttactttta
    52681 aagaatattg gaaaatattt gtataaaaat atatgtttta agtatttctt tgtgttagat
    52741 tttataaatt tattaataag aataacttat atattcccat gaaaacgata ttggatttaa
    52801 gtaagtttgt ctcataaaat gaaagtatat caattgataa tacttcaaat aagaagggag
    52861 aaaaaatgga agataaattt tatgcaaaag gcaaaggaaa taatggatat attaaaaatc
    52921 ttgaagtttg ttccttcaat aacttggatg gaacttgtgg aatgtttcaa atggctctgt
    52981 acaaaagaga tgaaaaatac tatttatatg gatgctgttt tggaggaaat aaaaaaaatg
    53041 gagtaatgat tagtgatatt acagaccctt ataatccaca atttataaaa cattttcaaa
    53101 tgttagaccc taaagagtat cctacaacaa caactccaaa aattcaaata gcagatgatt
    53161 taatgatagt agcaatgagt tgtggaagtg ggccaggagc acttgttgac caggctaaat
    53221 tagcaaatat taagtgtgaa gcaggaatta gaatatatag tttaaaagaa gaccctttaa
    53281 atcctaagtt tttaggatat tgggattgtg gtttaaagca tgtaatgggt gttcatagat
    53341 tcatgtacaa tggtggaaga tatgtacatt tatcaagtga ttgtgttggc tttgaaggcc
    53401 tggtttacag agtcatagat ataataaatc ctactaatcc agtggaaata ggtaaatggt
    53461 ggagaccaga ccaatatgca gatggatacc caaatagaac ttttgatgca ggagcacctc
    53521 attgcccaga atttatggat aaaggatggc ttcatggacc tccatttgta agagatggaa
    53581 aagcatattg tggttatgga ggagctggtt taattatatt agatgttgaa gatttaacaa
    53641 gaccaaaatg cttaggtgaa ttgccattta tgcctgcatt ttctagtaga cttgcaggtg
    53701 caagaactca tacagcatta ccattgccag gaagagattt agtcgttgtc caaaatgagg
    53761 gagaacgatt tcaattcttt aaaccagata atattacaga tgttcaagct atgaataata
    53821 tacatatggt tgatgttagt gacccaacaa aaccaacatt aattgctcaa tttccatatc
    53881 ctgaagtgcc aaaagatttc ccttatccta actttaatgt cgcaggacta ggaaaaccag
    53941 gaccatttgg tccacataat cttcacgaac caatggataa taagtcatgg ttagagcaaa
    54001 gaggagatag agtatattgc tgttatttcc atgcagggtt aagggtttat gatgtatcag
    54061 acccatatta tatcaaagag ctagcatatt ttataccacc aaatccaaat aaaacaccag
    54121 aagagtctta tttcccagga tttctaggac cacgcttggc agtaacagaa gatattattg
    54181 ttgatgatag aggctatatc atcatagatg ctttagatga tggattttat atattaaaaa
    54241 tgaaagaaga ttaaataagt actaaagagg gagagttgaa aatgaaaata ctaggtattt
    54301 cttttggaac aaaaaatgga aataatgaca caatgtgtaa agttgcactt aaaggtgcca
    54361 aagaagcagg ggctgaagta gaatttattc aaatgtcatc tattaacatt aaacattgta
    54421 ctggatgttg tgcgtgcgtt aagactttat tatctggaaa aggtagtata tgtgtattaa
    54481 aagatgattt tgaatggttg cttgacaaaa tgaaagatgc agatggtata gtagtttcag
    54541 accctatttt tgaagaaggg gcatcaggat tattccatac tataatggat cgttttggac
    54601 caagagcaga tagaggaaat aatgttatag ggacaaaagt tgcagaagct acaggaggaa
    54661 aaattccaga ttcaagaatg cttaatgata aagtaatatc atttatggga atagggggtt
    54721 ctgactgggg gacgagagtt cagtgtgaac atgctatgct agctctaata ccaatgtgga
    54781 aagttataga taacgcttgg ttcccatggg caaaggaatt agtaatggat gatacaaagc
    54841 ttgctcaagt tcgccaactt ggtttaaata tagttgaagc agcaaaagat tttgaaaaag
    54901 cttcatacca aggagaagaa ggtgtatgtc cacattgcca taatagatta ttctacattg
    54961 agccaggtac taaaaaggca atttgtgctc tatgtggtat agttggagaa ttagatacta
    55021 taagtggaaa aactatattt agttttccag aagagcaatt aggtcatgct catgacactt
    55081 taccaggtaa gtttatacat ggtgatgata ttaagaaaat ggaaggtcat tatgcagaag
    55141 ttcgtaaaac accagaattt aaagaaagaa aaacagctta taattttata gaaccattaa
    55201 ctaaagaaaa gcaagctgga gcttcattaa gataataatt aaaatactaa gactcttttt
    55261 taagggatgt tataggaggc ttattgtatg aatgaaaagc cgagctttaa acagattgaa
    55321 attattttag tagttatttg tttagtattt gcacattaca atgttagatt aactccagaa
    55381 tttattgcta taattatacc actatactta agttatttta tagtatctat tattgcaaac
    55441 caaatagcaa ttaagctatt agagataagg caaactaggg aagctactag ttacaatata
    55501 ggaaatggat agaaagaaaa aagaaatatt aacactgtcc atgggtattg ctttactacc
    55561 tcctttatgg gcagtattag caccttatat aggaataaaa actggagctg tagcactaat
    55621 ctgtgcagga ttatatgtaa ctaatggtaa taagcaaaaa gatggattaa aaataatgtt
    55681 tggtttttgg tgtggggatt tatgggcagt gttagccatt ttaattatgg aatatatgaa
    55741 cttcaatcaa aacctggaat tatttttaac acttagtata ttaggttttt ttgcagttgt
    55801 aattgcatcc tcatttgaaa aaataatttt cctaccatca tggctttgtg gttgggctat
    55861 aggtctaact attatgacag gtgaaaatat tattaattta caagatattt gtattcaaat
    55921 tgctatagct atggcagtag gaatttggta tgtgggtgta ggtgtagatt tgtttacaag
    55981 atatattttg agtaaggata agaaaaagtt aaattgaaag catgtgaata tactttggtt
    56041 ataggagaat ggttatgggt tttgaattta aaataatgag aagtttaata tatgtaggac
    56101 ttgccaagga agaatataga cctaagttaa tggactggtt atatcgtcac catattccag
    56161 atagtattag cacttttgga ccatattgta ctaaatatgc cttttatcaa gcatatccta
    56221 caccaaatga aggtgagcgt tttggtgcac gtaagatgca gctaacagaa cattattggc
    56281 ttgtagatga gcatatgcct gaaatggcaa atagaattat gacagaatat atgcctatgg
    56341 atgttctacg ttggcaagga tgtataccag atatagaaaa taaaagggtt catgaaaatg
    56401 cagaaagtgg agatgcagga cgtgcagtgg gtggagataa cggatgtcca ccatttatat
    56461 ttgcatttgt tccaataaac tgggaagaag attttagagg aaaaggacgt actgtacaag
    56521 atggaccaaa ctatcgttgg caatttatga ttaagtatcc agatggtgtt tccaaagaag
    56581 aaggagaaaa atggttctat gatgaggtag ttccatactt tacaaactgt tgctatgtta
    56641 atcgttttgt cagtagtaaa ataatgatta attatggagc aactgctttt gaccgtgtag
    56701 cagaactatg gtttgaaggg gaagaagagt ggtataaagc tgtggttgaa gaggcaaaat
    56761 cgtttattaa aaagccagaa tgggcacaag aagaggagtt cccatattta aaaccacaat
    56821 tcaatatcgc atcagtattt ttaggtgata tagcaactat ggatgcatac tcacagtatc
    56881 gtggatatat tccaatgaga taatatagtt tatggtaagt agaggaggta cttgctatta
    56941 ctagagaaaa agacttgttg tagctttaaa agtaggtgaa tatataattt taaaataaat
    57001 ttataaaaac taagattaat aaatatgaga caatagcatt agaataattt gatatatttg
    57061 ttaaaagaat atttagaatg tgtttaaata tgaagatgtt ccttttatga tgtcagatag
    57121 ttgaaacaat aaaactgtta tcatgaaggg aatattttta tgtctaggaa atcatttaaa
    57181 ataaaaatta aatgtgttta atgacatctt aaagtaaaag aaatattgtt agaaatatgt
    57241 agataatata gtatttataa tgtattatat taacaagttt acacttggat tataaaataa
    57301 aaagagtgat tctaaaggtt gtaaatacta tactgacttt gatacttcaa agtttagata
    57361 atgctgtagt tgttaataga aatacgttta atgagcataa ataatttatc taataagtag
    57421 gaaaatataa tgtatgtaat tggacaagta cctaaatttt taggaatgtt cagagacata
    57481 ctaaaatttt atgagggaaa agattagtta agaacaggag gaatagatat ggttttattg
    57541 attgttgata cacaaaaatt aataacaaat gaagagctat ataattttaa tgaatttgta
    57601 gtaaatttag taaaactaat tgatacagct agaaaaaaat atatagaagt aatatatgta
    57661 cgacatgatg atggaattga ttgtgaatta accaagggta aacatggttt tgaaatatat
    57721 gaagaattta agccatgtga tgaagaaaaa atatttgata aagatgtaaa cagtgctttt
    57781 aaggaaactg gtctattaga atacttaaca aataagaatg aaaaagatat aattattgct
    57841 ggacttcaaa cagattattg cattgacgct actataaaat gtgggtttga acatgggttt
    57901 aatataatag ttccatctta tgcaaataca actattgaca attcattcat gtcagcagaa
    57961 caatcataca aatattacaa tgaatttatc tggaatggaa gatatgcaga gtgtatttca
    58021 ttagatgaga cacttaaaag aatgaattag gtaagtatca ttttattata tgagataaac
    58081 tttaactttc ttagtttaaa atattaaagt taaaaggaac atttattatt agatatttta
    58141 tttatataaa ttaattgaag aaaaaaggaa ttaaactaca agttaaaaaa taaatttaat
    58201 acattagaat tataaataag gcacatcaat tttttttata ttaatgtgct ttttatttat
    58261 agtaatttag gcataattag tatcgttttg attttttaaa taaatttttc ttgagtttct
    58321 ttacctaaag aaaccttttt tatttataaa atatataagt gtaatatagt atctatttta
    58381 aataagttta atttttgtct caatctaaaa caaaagttat acttaaatga aattgaaaac
    58441 gataaaattc tcaaataaaa taagtattgt ggaggtgata aaaaaatcca atgataatat
    58501 gaagactaat cttaataaga aagtagggat taattatagt ggacaaagaa attgtatcaa
    58561 ttctccaaat tttattaaat agcacattga ttacgactaa tggattacaa gaggaatcta
    58621 attcatcaaa gagacaaatt acttatagaa taaataaaat caatgacatg cttaaagtaa
    58681 aaaatgtacc acttatatgt ttaagagctg ataaagatat tatcgttaga aaagaaacaa
    58741 aaaaagcaat caaagaaatt ttagaaaaaa actattctaa gaatacttat tactttagta
    58801 aagctgaaag acttttatat atgtatttaa tgctttttat taatatggag gatatatcaa
    58861 ttaatcattt tataaattct ataaaagtaa gcaggtctac tgtaaatcta gatttcaaag
    58921 atttaatacc aaatcttgaa aaaaagggca ttgaggtcaa gaacaatcgt ataaatggtt
    58981 attacttagt tggaaatgaa atggaaataa ggagagtgct tattaagagt attatagaaa
    59041 ccttgtctaa tgagaaaagt tcaagagtat ttgatatttt tattaaagaa tataatttag
    59101 acagttttga agaggataaa gaaattatat tgaagttaat aaaaaaatat gaaattactt
    59161 ttgttgaaga tagattaata gaatttatat atatatttat ctttctaaaa gcaaggatgt
    59221 attcaactag aatagttact catgaaaatg tagatattcc taatattgca gttatgagtt
    59281 caatgaaaga gtataaattt gctaaggaat tattagaaac atatgagagt gaagataaga
    59341 taaagtctca taatataatg tatattagtg cttggatttt aggtatatca gtaggaaatt
    59401 atgaagaaga tacagaagat aaggatgtaa tttcaaaaat tgtaaataaa atgatgacaa
    59461 agtttggata tttaagtgga gtttactata taagtcaaga aaaaatattt aaacaattat
    59521 attctcattt tagaccagca tactatagac ttttatttaa attacctatc tataatccga
    59581 tttgtgaaaa agtaaaagaa gaatataaat tagtttatag aattgtaagt aatgcaatga
    59641 aagagttttg cggattattt ggagaagaaa ttcaggaaga agagttagca tatcttacaa
    59701 tgcactttgc aacccttttt tcaaataaaa aagaatttga tggttttaga aaaagaatag
    59761 cattaatagt ttgttcaaat ggcgttggta gttcagccat tttacatgca gaacttacaa
    59821 acttatttcc tgatttacat tttataccaa tgtcggaatc agttggattg gaaaatatga
    59881 gtgagcctgt agatattgta tttactacta attataacac agagggttta aaaacagatg
    59941 tgccagtaat taaagtaagc cctgttatga ctacaaaaga aaaatataag attacacgtg
    60001 aagtgtatat tcaattaggt gatatttttt taagacaacc taaaatagat gaggttatga
    60061 atataataaa aaaatatgca aatgtaactt ctgaaagtat tctttcaagt gagttattaa
    60121 cttattttac acagatagag aattttacat ctaaagaagg agaggggcct atgctaagtg
    60181 agattactaa tgaaacacta gtaaaaataa aggtaaaagc taagaattgg gaggatgcta
    60241 ttagacagtc agcttctgta ttagttgaaa ataacaaaac aactgaaggg tatgtagagg
    60301 ctatgattaa ttcagcaaaa gcatctggac catacatagt aattacaaaa catgttgcac
    60361 ttccacatgc tagaccagaa gctgggtcaa aagagattgc aataggtatt gctactttag
    60421 atagtcctat tgagtttggg aatgatgaaa atgaccctgt aaaatatgta ttttgtctaa
    60481 gtgcaataga taacaatagc catctacgtg ctatgtctga actggttgag ctattggaag
    60541 aagaagagtt ttttaaggtt ttagatagtg ctaaagaacc caaagaaatt attgattata
    60601 taaaaacaca cgaattataa aggagaattt aattatgtat aaaattttaa ttgcatgtag
    60661 agcaggagtt ggctcaagtt taatgttaaa aataaagact caacaaattg ttaaggaaaa
    60721 taactttcca attgaagtag aacatggttc attagattca ttaaatggat tcaatggaga
    60781 tgctgtagaa acattaattg atgttgctga agaattaaaa aataaacatt tgaagtttga
    60841 tataattggg attcacaata ttgttgaccg taacgaaata aagacagcac tagaaaaatt
    60901 ccttgcttca aaagagggat aaatttagga taataaggag gaatatttat ggaatttatt
    60961 attagtttgc tgagtacacc tgctgttttg cttggtgtag ttgcatgtat aggattgatt
    61021 cttcaaaaaa aatcagctgt tgaagtattt acagggtctt caaaaacact tataggattt
    61081 ttaatatttg gtattggtgc ctctgctatg acaggagcac ttcaaaattt caataaattg
    61141 ttccaacatg gctttggaat tactggtgtt atagcttcac cagaagcagc tacagcactt
    61201 gcacaaggta catatggttt tgcagtttcg tgtacattaa ttcttggatt catattaaat
    61261 ttagtatttg cgcgtattac aaagtttaag aatattttct ttacgacagg tcacagtttg
    61321 ttcttttctt gtgtattagt tttaatcatg aaagcacatg gattcgataa tacaatcact
    61381 attttaatag gtggaactat cttaggattt atgtctgctg cactacctca attctgtcaa
    61441 ccctttatgc gtgaattaac aggtggtgac gaacaagcta ttggtcactt taacatgatt
    61501 ggttatggtc tatcaggtta tattgggcgt ttattttcga aacataaaga tgatacaaca
    61561 gaaactatag aatttcctaa atggttatca atattcagag atttcttaat gggtctatcc
    61621 atagttatgt taatcttatt ttatattgca acattaaaag ctggtcaagc atttactcaa
    61681 gaaattgctg gctcaacgca ttggttagtt ttcccactta tagaggcatt tacatttgta
    61741 gcaggtatgt caatattgat gtcaggtgtt cgtatgttct tagctgaaat aactgcagca
    61801 tttgtaatca tttcagaaaa gtatattcca ggttcacgtc ctgcactaga tgttccaaca
    61861 gtatttccat atgcaccaaa tgcagttatt attggattta tcacagcata tgctgcaggt
    61921 ctattagcaa ttgctattat ggcatcattt cctagtatat ttcctgtagt aattattcca
    61981 gctgctcata tatgtttttt ctcaggaggt acagctgcaa tatttggtaa tagttcagga
    62041 ggatggagag gggctattgc tgggtcattt gttgtggggt tattacttgc atttttacca
    62101 gttatactat atccagttta cgggactctt ggtatagagg gtgcaacttt ccctaatatt
    62161 gattataacg tagttggttc gatattacat aatattttac aatttataaa acctatttaa
    62221 actaaattat ataaagatat agtcatagtc actatatttt aaggaggata cattttatgg
    62281 aaaatacatt atctgaattt ttagataata tatctaatga attgtcagaa tttattgcct
    62341 caatagatga gacatccata aataaagcat gcaatttaat ttttgaagct gaaaaaaatc
    62401 atggacgtgt acatgtgact ggtatcggca aaccaggaca tgtatcagga tatatttcat
    62461 cattgctttc atctactgga acaccagcat atatattaca tggtacagaa gcagtacatg
    62521 gatcaagtgg acaagtagtt gaaggagatg ttgttattgc aatatcaaat agtggtgaaa
    62581 ctcaagagtt aaaaggaact cttgaaacac taaaaataaa tggagcaaag ataattggtg
    62641 taagtggcaa tgagagttca tttttaaaaa atatttctga tatcttcttg tttgcaggtg
    62701 ttaaacagga aggtgattgc ctcaataaag ctcctcgtgc ttcaattcta gctgaaacaa
    62761 tagtattaca atcattgagt gttgcactac agtatgctaa aggtcttaat acacagcagt
    62821 atttgaagtg gcatccagca ggaagtcttg gaaaatctat tagagaggga atataatttt
    62881 atgaaatata aaggaattat atgtgcaatg ataacaccat ttgatgaaaa tcaaaatatc
    62941 aattcacgag caacgtgtca actaattgat tatttgatta aaaatggaat ttatgggtta
    63001 ttcattttag gcactaatgg tgaatgtcat gtgttgacag atgatgaaaa agttgaattt
    63061 gcaaaaattg ttattaatca tacaaataat cgtgtgcctg tttttgttgg aacaggtggc
    63121 aattcaactc gtgaagttat agatttgtct aagagaatgg aagcggttgg agcaagtgca
    63181 ttatctatta ttacaccata ctttgttaca ccaacacaac aggaattaat cttgcattac
    63241 gaaactattg ctaattcagt taatttacct attataatgt acaacatgcc agacaagact
    63301 ggaattaaca ttgaaccaga gtccgtgtgt aaattgtcga agataaagaa tattgttgga
    63361 attaaggata gtagtggtaa acttgacaac atgaaagctt atattgaagt aactaagaat
    63421 gaggatttca gtgtattttc agggtcagat tcacttattt tggatagtct aaaagctggt
    63481 agtcaaggtg ctgttgcagc aacagcaaac ttccttacaa agattgatat ctctatctat
    63541 gaaaacttta tgagagggga tttggaagaa gctcaaaaag ctcaaagtag tatagaagaa
    63601 tttcgacgta tcttaaagct tgggacaatt ccatctgttc tgaagaaaac tattgtattg
    63661 aatggaattg atgttggaac tgcacgactt cctgtaattg aaccaactgg agacgcactt
    63721 gaagaaatta aaaacgtagt tgaaaattat agagaaatgt tttaataaaa gtagtgagaa
    63781 cagataacat aactggcgga agttacatta gtatataatt aatatatgta ttataaaata
    63841 tggctaaaat tattggatag gtaaagtggt ttgatgagta tagaggtgat aatatgaaaa
    63901 agatgaatat aaccaaaaga atggcagaat gtggtgctct tgcaattgta agagaagaaa
    63961 agcttaaccg tgcatgtgaa attgtggaag gttgtataaa aggtggtata acagttgtag
    64021 aaatgagtta taccctgaat aatgctggag aaataattca aggacttaat aaaaagtatg
    64081 gtgaagctct ttgtgtggga gcaggaacag ttttagatag tgaaacagct cgtcatgcta
    64141 tattacatgg agctcaattt gttatttcac caaattacaa tgaagatgta gcgaagattt
    64201 gcaatagata tcagattcct tatgctccag gatgtacatc attgactgag tctgtagagg
    64261 cactgagttt aggagcagct tttataaaag ctttcccaat atcagatttc tatggaccta
    64321 aactagtaaa agtatttaag acaccaatac ctgatatgcc agttttagca agtggaggaa
    64381 ttaaccttga aaacctacat gtatggttag aaaatggggt tgacgtttgt ggatttggtg
    64441 gtttattaac taaaggtacg gttgaggata ttgctaaaaa tgctaagaag attcgtgaaa
    64501 tcattaaaaa tataagagaa gtttaatatt gggtttgtat catagcagtt tataattgct
    64561 atgatacaaa ccctttagta tttgttatct taatttaaaa tatagatgaa attagtaatg
    64621 caactatata aggattgcta atcaatatat attttttatg tatttatgaa tttgtaggta
    64681 agtacaaaag ataaatattt tatttataaa tttactttat tgtttgttgt gaaacatctt
    64741 ttagcctata tttatgacct tcattgatac tataaactaa tgctaaagtc ataattatag
    64801 acagaattat taaaataatc caagccatct tgtaagaacc agttaagtca aagatgcttg
    64861 caatagctgg tgtacctata attgagccta tacaagtgca agaggccaca aacccatata
    64921 tttctggata atgttttgta ccaaatattc tacttgttaa aacaggggta gttactgtac
    64981 ctatacaagt tccaattcca aagaagaatg tcatcaagta aagcattggt ttaaattcta
    65041 taaaaagcaa acaaactata gatattataa aacaaatatt gcctaataat actcctgcct
    65101 tagagcctaa tttatcaaat acatgcccca atacaacctt accccatatt gctataaata
    65161 agtacgtaga tactattgag gctgttaaag aagggttata tccactgtct tctaaatatg
    65221 ctgggatatg accaagaata catccagtaa gcaatattaa agtaacacca aatgtgaaag
    65281 tccaaaatac tggtaacttt tttgcttctt taagagagat attccattca ttttttatag
    65341 tatttttgtt agtattatct tgatgtctat catatccata aggttgcaaa cctttgcaag
    65401 aaggaaactg acgaacaata aagattgtag ctggaaccat aattaaaaac ataacaatag
    65461 caagataaac ataagtttgt ctccatccat aattagtaat caacttaact actagagggc
    65521 taagtaatgt tccactaata gaagtaccag ccattgaaag acttagagca agacctcttt
    65581 tttcataaaa ccagttagag attaatatgg atacaggcat tttagttgca ccataagtaa
    65641 aaagaccaat aaagaatgat actacataaa agtggaaaat tgatgttgct aatgaatata
    65701 aaccgtaaaa tatagaaagc cctgcaacac agataacatg aattaaatgc atatgtttag
    65761 tagacataag ttttcctgtg atagatgcca taaacataca cattagtgaa attatagcag
    65821 tagatagagt gtaagcactt ctagtaaaac caaggtcttc agttatgggt tttataaata
    65881 aacttgctaa gctaactata ggagcatatc ctaaagccat tactataata catgctaaga
    65941 gaatccacca tccataaaat attttactat ttttaactgt gttttccata ttataaactc
    66001 tccttactca tattttttag ccattgatat gcacattgag catatatagc tgaaccgaga
    66061 gcaagacatt tgtcgttgaa cattactttt ggattatggg caacatacca tttagaagta
    66121 tcttctatac cagcacctaa gtacataaaa catgtaggaa ccttttcaga aatataagca
    66181 aaatcttcag aacccatagt gtgattaaat tctgacattt taatgttttt gtctaattca
    66241 tagatataag acatgaattt tttatttaat tcatcattac atattaaagc aggtacattt
    66301 gataaaactt caatatcagc actacagcga taagttatag agacactttg tacaacttca
    66361 ttaattcgat ttattaataa atcccttgtc gttggagaaa atgtacgaag tgtaccctgt
    66421 aaaactgcct cattggggat gacattacaa gcctcgcctg atttaaactg acccaaagtt
    66481 agagttgcta gttcttgagg tggacattct ctactaatga gctcttgaag agctaaatat
    66541 atgtgaacgc ctgtattgat agggtctata caattttctg gttgagaccc atgaccacct
    66601 tttcctttaa tggtaatctt aaatccataa caagaagaca taaattcttt gccataaacc
    66661 acagacttat ttggcatagt aggcactaca tgcattccaa atgctgaatc tacttttgga
    66721 ttctcaagaa ctccttctga aatcatagcc tttgctcctt caaaaacttc ttctcctggt
    66781 tgaaacatta attttactga cccctcaagt tcattttctt taagctttag aatcttagca
    66841 gctcctagaa gtattgccat atgagcatca tgtccgcatg catgcatata attattattt
    66901 gatttaaact ccagctctgt ttgctctatt actggaagcg catccatatc actacgaatt
    66961 aatatacatt ttccgctatt cttattacct attaaacctg ttattgaata gccattacaa
    67021 ccaactttat attcaatgtt aaattcttct aatttttttt gaataaagtt gactgtattg
    67081 ggaagttcca tacctatttc tggattttcg tgaaaatgtt ttctccagct aataatctca
    67141 ctttcaaact gttttgctaa attcataaac atatctgaca tatttatctc tccttttaaa
    67201 gattttaagt tttaattata cttatccaaa aacttgtaat taatactaag ttgtgttagt
    67261 tttatgatag catattaaat ttgtcaatcg gtattagcaa aaaaacatga ggtataagga
    67321 aaaggttata tcaatttata atatatacta gtttaggggg atatgatgaa tttaaattat
    67381 tttcaatatt tagttgaagt tgcaaatata ggctctatta gtaaagccgc agaaaaaaat
    67441 tttgtatcac aaccatattt aagtggtatt atcaaaaaaa tggaagatga agtagggttt
    67501 ttgattttta aaaggacaaa tcttggtata gaattaactg aaaaaggcga gatttttata
    67561 aatgaagcaa aaaaaatttt aataggaatt gaaaatataa aaaatataaa tacaaagaat
    67621 gaagaagggt tgtcaatcgg tatggtctac tctttatttt ttacgaaatg ttttctaaaa
    67681 tttaaaaagt ttaaacctac agacttacaa gataaagtct atgaaatgag tccaatggaa
    67741 atattcaaag cattaataga taataaaatt aggctagggc tattttccta tgcgactaaa
    67801 aaaaaggaca aatattggaa tattgctaag aagcatcatc taaagtgttg gagtatatat
    67861 gaagatttac atccttatgt aattatgtca agaagccatc ctttagcatt aaatgattca
    67921 attacaatat cagatttaaa cagtaatcct tatgtagttt atgataaaag cgaattttta
    67981 atatatctag atatgatagg tataagaaag catccagatt tgcttttagt ttcagataga
    68041 ggtagttttt ctgatgcaat attagataat aattatctag gtataaccac ttatttttca
    68101 gagattaatg agcaagataa caaatttata tatataaagt tagaagattg tgatgaaact
    68161 ataggaatag gttatgctat attagataat tataaattga gtcaaagaga aaaagatttt
    68221 attaaattta tcaaaaataa gtaaaatatt ccatatttaa tgtgggtaga ttgtcatatt
    68281 tcaaatcaat aaataattag tatataccat ataagtaatt gcttatatgg ttatttttag
    68341 tgaagcaatt actttagaaa atgaaacaga taattatgta ataaataaga gatagaattt
    68401 ttatttatct aacattaagt ataaaagaaa attactatga cttaataaaa taagatatta
    68461 acaatgaaat tcttgataaa taccttttaa tttaggcatt ataaagttta attgtacatg
    68521 tgttacatat tttcttgaaa tttataatta atattaaact tcctcagttc aataataata
    68581 tactaaattt ataagttagt attattcata tgtaaagctg agtgctttac taatttatat
    68641 cgcataaaat ttcagcaatt ttatcttaac ttttctatat aaaaagaact gctttaaaac
    68701 ttggggtatg atataatttc attaaaagaa gacggttttt agtaaatact agaaaataat
    68761 atgtttttat agaaaaagtt aaatatggat ttaagtgaat aagaatcaat aagtaaggta
    68821 ttaaattatg aatagggtac aaaacattac tgaattaaaa gttttagagg gagaagttta
    68881 gcaaggatgt gtgaaaatta attctcaaac aggaattatt gtatatgaga taaatttaca
    68941 cacattgaag attcactttt atttatacta aaaagtagat tgtcttaaag gtgggtctaa
    69001 agataggagg ataatcaaaa taatgaaaga agacattgga ttacatcgaa tgtgtaaaga
    69061 agaacaagta acaaagaatg aagttatttc ggaggatatt ttaaaatcca tgagtatggg
    69121 agtcagtaaa cattttttag atgattcatt tacaatacta tggggaaata catcattcta
    69181 tcaattattt ggatatacag aagaagaatt tttatttaag ttttctagtt taaaagacta
    69241 ttatgaacag aatctttata attttgaatc tatgaaaaat cattttttag aggcttatca
    69301 tcgtggagaa aagagtacag aatatcaaat ttgtatttca gcaaataatg tagaccctgt
    69361 ttgggtaata atgaatggta tattaattga atcagaagaa aatgaaaaat caatggtcta
    69421 tatattgtat tcaagaatta atgaattaga aataaagcaa gaagaagcat ctaagatgga
    69481 aaaagagagt acagagaatt ttaaatggat gatgtcagag tatgctggaa atgtttatat
    69541 tagtgatatg gatacatatg aattactata cttaaataag caatcatgta atatcttaca
    69601 ggcatcagta aatgaactca ttggaagaaa gtgttatgaa gtaattcaag atagaacttc
    69661 accatgtcca ttctgtacaa atccatattt gaaaaaaaat gacgtatatg aatgggaatt
    69721 ttataataaa aaactgaaac gaacgtttat gattaaaaat cgtatgttga attggaaagg
    69781 gcgtagagct cgtatagaat tatcttatga tatgtatagt gctgaatata aattagcaaa
    69841 aaaagatcaa gaaagagaat caattttgaa aaccattcca gcaggaatgg ttcgagtaga
    69901 tgcaagagat tatcttacta tactttggta taatgatata tttttgaata tgattgaata
    69961 tacaaaggaa cagtttgcag aggaacttga taatcaatgt ggatatttat tttctgaaga
    70021 ttataaacgt agtaagaagc tagcacaaga tttaaaggag tctggggata atgttgtatt
    70081 tgaagcaaga atatacacac gttcaaggga agaacgtata tggaccgtta cattgtgtta
    70141 tgtcagtgga gaagacagct gggatggaat accatctttt tatagtatag gtattgatat
    70201 aacaaaggaa agaaaacaga ttgagaaatt acagtatata tcagagaaag atgcattaac
    70261 aggaatttat aatcgtgctg agactgaaag gcagattaag aagtattttg aagaaaattt
    70321 aaattctatg ggtgcactgt ttatgattga tacagataat tttaaacaga ttaatgatac
    70381 agaagggcat atgattgggg atatagtgct tactgagatg gcatcaggta tgaaaaaaat
    70441 aatgtgtgac agtgatgttg ttggaagaat aggtggagat gagtttacaa tttttatgaa
    70501 ggatatttcc tcagttaaag atgcagaaaa gaaagcagaa gaattattgt atatgttccg
    70561 tcacctattt cagaaagaaa aaagttctct aaaagtaact tgcagtatag gaatagctat
    70621 atatccaaaa gatggtacta catttaagga aatatataca agagctgata aagctctgta
    70681 tcaagcaaaa aacataggaa aaaataatta tgtaatctac aatcaagatt cttttaaaga
    70741 attagaggca tttaattgtt cttctcttgg tacagttatt gattcagaaa agcaatatgc
    70801 tgaatgtcct gataatctta cacgttatat attccgtatg ctttatcaga cagataatat
    70861 agatgagacg attaatatga tacttgaggt tgttggtaag cagtttaatg taagtcgtgt
    70921 ttatattttt gaaaatacag aggatggtcg atatacttct aatacctatg aatggtgtaa
    70981 tgaaggaatc tcttctcaaa tggcttattt gcaaaacaac aattatcaag actatgagga
    71041 ttatgaaaag ctttttggag atgaattagt tttttattgt cgagatatcc acacattaag
    71101 tcctaaacta gaagaattgt tttctagtca aggtatccac tctacacttc aatgtgcata
    71161 taaagaagat aaagttttca gtggatttgt tggatttgat gaatgcacag gattacgtct
    71221 atggtcacaa gaggaaataa gtacattatc attgatttct caaattatat caatgttttt
    71281 gcaacgtaaa aaaaataaaa aatttaatca acaaatgcaa caatatcaga atattttaga
    71341 taatattgac cagtgcattt gtgtgattaa caaagaaaat aattttttat tgtatgtaaa
    71401 taaaaaattt aaaaatacat attctaaatt acaaattgga caaccttgtt atcttgatgc
    71461 tgtagatatt cataagatat ccattgcatg gaatcaaaaa gaagcatact tatgtattag
    71521 catgatttag tggtaaggta aagtttgtat tgaacttaaa ctaaatttag ttttaaataa
    71581 aatattattt tggttgatta ctgaaaatac tagaatctat gttaatgaat tatactaatt
    71641 ttgtaccact attaaaatat tgtttgataa aaataaataa aaatcttaaa acagttggtt
    71701 ataaaaaatt aactgttttt tattgttttt atatttgtga caaaatagac acatgccatt
    71761 tagaatgtga caatatagta gttgtttaaa agagatgcaa agcattatac tttaattaaa
    71821 gtatagtgta tttttgcttt taaaataaaa acaattaggg ggagagttat aattgaaaaa
    71881 aataatatat gtattattta caattgaact attaatagta tctttattag gtctaaatat
    71941 agttaaagat aatgaaatta ataatatttt atataatgat acaacaagta tatctgtaat
    72001 gtttaaagat tataaaaagt tcaacaaaaa ttatagtaat tggattaaag atattgcaga
    72061 cgaaaataat gtgactatat caaagtatgt ttttaataat aatgaaaaat tatctatata
    72121 tacaactgat acaagtttaa ataataatat aaagttaaaa tcaggagaat ttcctaatac
    72181 taacactaat tcaaatgaat ttattagtaa tataaacact aataatagta atcaagtagg
    72241 taaattctca actatgagta aagacatatc aattttgatt agggattttg ataaaataag
    72301 tgaagttgga gaatcaggaa ttttatatat ttcaactcaa aataaagata caataaataa
    72361 aatattaaaa gagttaaatg tagacagaag tatttttgta gcaagactac atgatagata
    72421 tgttaattca aatttatact taaatccttc aataattaga gatttaatac taattatcct
    72481 ttgcttcttg gccacaataa tacattattc aattagtgtg agtagagagg cttctatttt
    72541 aagattaaat ggatattcta agttagatat aattactaga gttataagaa gtatacttag
    72601 aattatgatt ttatcatcta tcacatcatt attaatatat ataatctata ttttaaaatt
    72661 aaatttgggt gttagagcat gtttatactt tgttatattt tcgattatat gtattttgat
    72721 aaatattttt atatctatat taatagtggg ttttaatatt agaagttcta aatatgtgct
    72781 aactctaaat ggaaaaaaac cttatggttt tgtaaacata attcatttca cattgaaaat
    72841 tgtatttgta tcatttctta tattatccat aaataactgc attttaaatt ataatattct
    72901 acaaactcag ttaggaaatc tatctgagtg ggataaagca aaaaatgttt ataaccttac
    72961 tcttaaagat acaggtgaac aatctttagg aaaggaagaa gtaataaaaa atgccaagat
    73021 taaaggattt tataaaagtt tagtagaaga aaaagatgct ttcttaattg atacaactaa
    73081 ttttgaaaaa cttgaaaatg gaagttacat gtatgaaata aattcaaagg gtaaaaaacc
    73141 agaagtaagt caatatggta aaaatataaa gataaataaa aactatctta aagttaatcc
    73201 tatttactca aatggcaaag aaatatttaa tctaattgac tatgacgata agactataaa
    73261 tttattagtt cctaaaaagt tacaggtaca tgaaaatgag attaaaaaag agtttagaga
    73321 attattttat tttgaaaaaa tagaagtaga aaatatgtat aatgaagaat taaatagaga
    73381 tttaaataaa acaaagaaat cagatttaaa tgttaatata atatatgtag ataataatca
    73441 aagctacttt acttataact cacttgttat ggatgacaat aggaatctca ttaatgaccc
    73501 aatagcaata gttgacatag ataatattga tgattctttt tatctttcct atatatctag
    73561 atgtgtatat tttaattcta agaaattaga tgctcttgca gatataagta aaattattga
    73621 agctcaaggt gtagaagcga atatccaaag tttacgtgct gtttacaatg agtatggttt
    73681 agaaataaat aaattagaaa aatatttgaa tagtgagatt tttactataa taataatagc
    73741 aatatcaaat ttaatgatta cctacaatat agtagttagt tactatgaaa gaaacaaata
    73801 taagttatat attaaaaaaa tatttggtta ttcagctaca gctagaagta tattgttaat
    73861 aatttcatta atattaacaa atgttattcc aataggcatt atatcaacaa gagtagattt
    73921 aagcaacaat attgtcttat ttggacttct tatattagtt gtagaagtaa ttatatctat
    73981 tatattagac aagattatct caaatagttc atttaataaa attataaaag gagaacatta
    74041 aaaagatgat ttcattaata aatatatcaa aaaaatttaa agataaatct ataatagaca
    74101 aatttaacct agaaatttct aagggcgaat ttgtagcgat aacaggaaat agtggctcag
    74161 gtaagacaac tttacttaat ataatgggat tattagaaaa acctaattct ggtgatatta
    74221 taataaataa tataaaaaat ccaaatagta aacaaataag aattttacaa agggattttt
    74281 atggttacct atttcaaaat tatgctttaa ttgagaatga aaatgttgaa agtaacttga
    74341 aaattgcttt aaagtatcaa aaaaatatta atcgtgatga aaaaataaat tacgtacttg
    74401 agtctgtagg tcttggaaaa tacaataaaa aaaagatata cgaattgagt ggaggagaac
    74461 aacaaagggt ggcgttagca agagtaatat taaaagagtg tgaagtaata tttgcagatg
    74521 agcctacagg taatttagat aaaaaaaata gagatatagt atttgatata cttaaaagtc
    74581 taaataataa tggtaaaact atcgtattag taactcatga tttagagcta tcggagcagg
    74641 tagataaaat tataaagttg taataatatc aattacaata aatatgggaa agagattaca
    74701 gctaaatcaa attcaacaat actaggaata taatttaaat attaaataaa ttagtagttt
    74761 gtaaatataa gtataatatg atattataaa ggctcaaaag aaattttata taataagaaa
    74821 tctaaaaata ataaatggag gacatataaa tatggaaaat ttaccaaatt gcccgaaatg
    74881 taattcagaa tatacttatg aagatggaac tcttttagtt tgtccagaat gctctcacga
    74941 atgggcatta ggattggaac tagaaggaga ttcaaatatt atcaaagatg taaatggaaa
    75001 tgtattaaat gatggagatt ctgttacagt aattagagac cttaaagtaa aaggaagttc
    75061 atcagctata aagattggaa caaaagtaaa aaatatccgt ttaatccatg actcatcaga
    75121 tggacatgat atagaatgta agatagatgg ttttggagct atgaagctaa agtcttctgt
    75181 tattaaaaaa gcgtaatatt ataactaaac tatattgttt attattttca agtatagtat
    75241 aaaaccataa gaatctatta agtaataaaa atttacttat ttatctttat attttcttat
    75301 ggttttatta aatatagatt ttattgaata ccaaaatatt ctctcctctg caaataagcc
    75361 aatcttattt atttaaacta atatattttg tactatttat attaaaattg taaatttaaa
    75421 tgctatgtta tttagcttta tatagttaat ttacaaattt ctgattttat aaattgtatt
    75481 tattatatct ttaatgctaa aatcattaaa agtttcaatt tctgtaaaga tttacaattt
    75541 ctcaaataca ttatgaatat gtgaaaaatt tattactaca atactacatt taaaataagt
    75601 attagtataa aggtattgtt acaagtataa agaaatttat gtatactatt ggaataaata
    75661 ataccttaaa ttataattaa gaatagaact gttaaataag agatttgatt atcatttgat
    75721 tatagataat gtactattaa aaataaagtt taatgaggag aaaaaatatg ataagtatac
    75781 ataaaaatat atatgatgat aagttatttg aggagctaaa gattgactgt aagaagtgtt
    75841 ttggactttg ttgtgtagct ctttattttt ctgcgtcaga cggatttcct attgataagg
    75901 aatcaggaaa accctgtatg aatttacaac cagattttaa atgttctgta catgataatc
    75961 ttatgaaacg aggttttaag ggttgtactg catatgattg ttttggtgcg ggtcaaaaag
    76021 tagcacaagt aacatacaag ggtatagact ggatgcaatc accagaacta gcaaatcaaa
    76081 tgtctgaagt atttttaatt atgagacaac tacatgaaat gttatggtat cttaaagaag
    76141 catctgtatt aaatatatca gatacaatca aatcaaaaat taatttaaga atcgaagaaa
    76201 ctgaaaagat aaccaatatg agttcagaac agatagttga tttggatatc atatcacata
    76261 gagaaaaagt taattcatta ttattgcaag cgagtgagtc tgtgatggga aaggttaaat
    76321 cctttatcaa aacctctact cttaaaaaca tgaaaaaatt aagcaaaaat atagatttaa
    76381 taggagcaga ccttagaaaa acaaatctaa taggagcaga tttaagaggt agatttctaa
    76441 tagcagcaaa tttaagaaat acagatttga gtggggcaaa tttaatagga gcagacttaa
    76501 gagattgtga tattagaggt gctaatcttg aaaatagtat atttttaaca caattacaag
    76561 ttaatactgc aaaaggagat tctagtacaa agttgccagt ttcactaata cgtcctaaat
    76621 actggggaaa ataattaaat taatacataa ttttacaaaa tatgaacttt tttgaccttg
    76681 attcatatta tacattagag attctttata agaatttctt aatataatat agggagagat
    76741 ataaaaatga gttatcaaaa ttataaatgt gaaagagatt atgtgtatga tgattataaa
    76801 tacaataagt gtaacgagta ctataatgat tatgaggaaa tgacacatgt tcatgaatat
    76861 tcagaaagtg ttaagttagc agaagaatgt gaggatagac ataaccaccg tgcagcagga
    76921 gttactggtg aagttatacc aataaatgga ggtacaaatc atgttcataa aataaatgat
    76981 aatgtagatt tccttgacca tttccataag atatgtgtta caacagggcc agcaattaga
    77041 atacctggaa ctgataaaca cattcactta atatgtggag aaactactgt taatgatggt
    77101 cattgccata aattcctttt tactactcaa atagaagctc ctttagtata agctgatgtg
    77161 aactattgta gtaaatttgc tagattatag tatataaatg ttaaaaatat ataatagata
    77221 atatttaaac ttaatgagga ctatgatatt taaccttcgt agtcctttct aagttttgta
    77281 taaagaagtg ttatacaatt aaaagtgata tagggaggaa atttttatga aatctggaat
    77341 aattcttgaa atgaaacaga taaattcaac agaatatgac gaaatgttgc aagtctggga
    77401 atcgtcagta agagcaactc atgatttttt aacagaaaaa gatattaaat ttttgatacc
    77461 attagttgaa attggactta aagaagtaga aaatatagtt tgtataaaag ataatgatat
    77521 aataaagggc tttataggaa tagataaaga taaaattgag atgctcttta tagaagataa
    77581 atatagagga aatggtattg gtaaaaaatt aataaaatat gctatagata aatacaatgt
    77641 aaaatatgtt gatgtaaatg agcagaataa aaaagctgta ggattttata ttcatttagg
    77701 gtttaaagcg ttcgataggt ctgaaataga tggacaaggt aacccatttc caatactaca
    77761 tatgaaatta atttaaagtg atacttctca cttattcgca aaaatattta cttaaatttt
    77821 ttatacaaat aaaagtcgtg acagtcttag gacacgactt ttttactaca ctattaaatt
    77881 gaaatttatc tgttctatat tatgattatt agatgtagtt tttttatttt aactatgtac
    77941 ttaaatacca agtataccca tacctatcaa gtacttcagc agcaccatca ttttgatgtg
    78001 gtggtgggtg tggtaaaaat tcttgtacaa gcgtaccacc ctcggcaagt tttatagata
    78061 tttcttctat cgtttctcga gatggtagaa caacacaaat cgccatgcca gaaattacaa
    78121 caggcttgtc tgtatccgca cccattatac aaccttcata ttttgtgaaa gtaacacttg
    78181 cgtgcataac aggattgttt ggattgcttt ctaatcgtgc aaatatagtt tttcctcctc
    78241 cgaaagcctc agcataaaaa ttaaatgctt tttcacaagt accattaaat attaaatatg
    78301 gagttagcat gacaaatata tcctccttga tatagaataa tttatagttg cacctgataa
    78361 tctaaattct gatttagtaa attgtatcat agggaaacct tgatgtatat aattttttat
    78421 tattagatta tacaaatcta ttaatttaaa aaaatgttat atgtaaggaa ggtttatagg
    78481 ataaaatata ataaaaagag taaggtgaca ttataaaaaa tgtatattga cattgacgct
    78541 acgtaattgt atatactttg tcttaaagga gatggtaaat atgaatatta tagaaattcg
    78601 ctcagataaa atatacaaga agataatgga tgcaccaata aacaaaaaag aagatatata
    78661 catatatgaa ttgatgaagc cttttgaatt taagtggaag tgtatgaatg ttccaatagt
    78721 tgctaaacaa aaaggcggat atgatgtaat tatagcaagt gaaatgttag gagttttatc
    78781 gcctaaggat attgatgaaa agcaaaaaaa gaacataaat atgttatcta ctgataaaat
    78841 ttggaacact tgcaaagaaa ccatagaaaa ctctataaat gcttttataa aagaagggta
    78901 tgatttaaat attaaggatt ataaatattc aatattatta gctaatccaa atagtcctta
    78961 tacaatatta agtgatggat attggggtga tggtgggatt cctggatata tatttctatc
    79021 attggttcct aatgaatata ctatcaatag attaccagta ttaatagcac atgaatgtaa
    79081 tcacaatatt agattccagt ttatagagtg gaataataat ataacattag aagaaatgat
    79141 gataaatgaa ggtattgcag aaaattttgc aaaatggatg tttggagagg aaatggtagg
    79201 accttgggtc agtagaacag atatcgaaac actaaacact tatataaagc caataataaa
    79261 aagtgcttta aaagaaactg gatttcaaaa tataacatct tatctttatg gtgatgacat
    79321 agctaaaatg caaggatatt ttccagtagg gttgccttat tgtgcaggat atgcttgtgg
    79381 atattatatg attaagtatt atttagaaaa gacaaataaa tcaataatcg aagctacttt
    79441 attgccttat agtgagataa tagaagcagt agaagagttt tgggaataaa ttttattaat
    79501 tgcatgattt gaaataaatc atgcaattat tttattttta agaaaaaata aattgacaat
    79561 aaattataag aaatatacaa taatatatat aaagaaatat ttcaatatgt aaaagtttta
    79621 agatttcata tctagagtac ctaacattac atgttagttt cttgttactt atctaattaa
    79681 tttatatttc atagttat
//
LOCUS       DAMJVM010000005        78866 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.005, whole genome shotgun sequence.
ACCESSION   DAMJVM010000005 DAMJVM010000000
VERSION     DAMJVM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 78866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78866
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.005"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(86..913)
                     /locus_tag="PC523_000386"
     CDS             complement(86..913)
                     /locus_tag="PC523_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2473954.1"
                     /translation="MKIKDNKTVFHVFVRIFLVAVLILTGTLLEEKINNVPVENISSK
                     GESTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYDKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENISPKNFWQENL"
     gene            complement(961..2397)
                     /locus_tag="PC523_000387"
     CDS             complement(961..2397)
                     /locus_tag="PC523_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HDF2473955.1"
                     /translation="MNTKNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDDLNNYFSGKFDKDTMVNLIRVTMGLDVTGDTDKNLKDSLHEAVNLDEVKRPIIS
                     IISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFIGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIDATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNRKENKYKK"
     gene            complement(2484..3353)
                     /locus_tag="PC523_000388"
     CDS             complement(2484..3353)
                     /locus_tag="PC523_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2473956.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKIMRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            complement(3511..4164)
                     /gene="phoU"
                     /locus_tag="PC523_000389"
     CDS             complement(3511..4164)
                     /gene="phoU"
                     /locus_tag="PC523_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDF2473957.1"
                     /translation="MVNNSLDISIDTLKEQTVLMIEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEIDKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIDGKMVEIG"
     gene            complement(4192..4956)
                     /locus_tag="PC523_000390"
     CDS             complement(4192..4956)
                     /locus_tag="PC523_000390"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDF2473958.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGSISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRIHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTIFTTPVDKRTEDYITGRFG"
     gene            complement(4975..5799)
                     /gene="pstA"
                     /locus_tag="PC523_000391"
     CDS             complement(4975..5799)
                     /gene="pstA"
                     /locus_tag="PC523_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDF2473959.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGKMNFNFL
                     FSNYSASGDGGILPMIITTLYTVLVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSIGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            complement(5801..6718)
                     /gene="pstC"
                     /locus_tag="PC523_000392"
     CDS             complement(5801..6718)
                     /gene="pstC"
                     /locus_tag="PC523_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDF2473960.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKLAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGANWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPMIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            complement(7665..8345)
                     /locus_tag="PC523_000393"
     CDS             complement(7665..8345)
                     /locus_tag="PC523_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2473961.1"
                     /translation="MQTKILIIDGDKDNCQKLKKFMEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILTDGDGWTLCKKIRNVTTCPIVYITYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(8395..10410)
                     /locus_tag="PC523_000394"
     CDS             complement(8395..10410)
                     /locus_tag="PC523_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2473962.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTHKSIRASNG
                     ILNIDNETFTKSKLVFLIDDWEFYNHKIFKPNDFIEQDIKPEYVFIGQYPDFSMRKGN
                     QSPYGNATYRMRIKNDGKNRLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NLVVSFIAKKDTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRVLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKLYLYFGCMCIFFAIHVSYPFVHLLGLPLVNFTYAVEDSSY
                     FIAVLCIICINGSISKIEENKLYRFLILPLGITMSIMSIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAITSNRFEP
                     IFTGWQTEYCGFIIVILFSVMMINKNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KKFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNDFIKEIYENNLPDTQACGVNFETYILEKKIYAKINKEMLI
                     RAFENLIYNALTFTPFDGKIILSMDEEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNNPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            complement(10482..11159)
                     /locus_tag="PC523_000395"
     CDS             complement(10482..11159)
                     /locus_tag="PC523_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2473963.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     regulatory      complement(11739..11822)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(12055..12906)
                     /locus_tag="PC523_000396"
     CDS             complement(12055..12906)
                     /locus_tag="PC523_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2473964.1"
                     /translation="MFKKKIGVLLASTILVGALAVGCGSNSGGSGNSGNSGDSSKVSV
                     SGSTSIGPLMETIGEKFQEKNKGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEE
                     KSAGLKETKIAIDGIALITNTANPIKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVS
                     REEGSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKV
                     NSLKINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQAIVSEK
                     GFIKVNQ"
     gene            complement(13159..14943)
                     /gene="pepF"
                     /locus_tag="PC523_000397"
     CDS             complement(13159..14943)
                     /gene="pepF"
                     /locus_tag="PC523_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDF2473965.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSNKEEIEKDISKVKNLIQEVKEYK
                     GKLSDSKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDDKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTVPENVYDMLAYADMQFPEIEDEDGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVYRQTMFAEFEKIVHERVENGEPLTADEFTDIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(15009..15674)
                     /locus_tag="PC523_000398"
     CDS             complement(15009..15674)
                     /locus_tag="PC523_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF2473966.1"
                     /translation="MIDIGSKEIALRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGVAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKSQLIIRNMEIECRNEEYWKVNFFVSYAKRIHLQDVMKEINMIEGVINVD
                     YLD"
     gene            complement(15825..15986)
                     /locus_tag="PC523_000399"
     CDS             complement(15825..15986)
                     /locus_tag="PC523_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HDF2473967.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(16064..16573)
                     /locus_tag="PC523_000400"
     CDS             complement(16064..16573)
                     /locus_tag="PC523_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDF2473968.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKGL
                     SIIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            16780..16998
                     /locus_tag="PC523_000401"
     CDS             16780..16998
                     /locus_tag="PC523_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2473969.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            17016..18962
                     /gene="feoB"
                     /locus_tag="PC523_000402"
     CDS             17016..18962
                     /gene="feoB"
                     /locus_tag="PC523_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2473970.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLGEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIALNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFASFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIVEALIGGIIIALALFILFNKFKSTKNGSVCSGCS
                     ICSGCPSANSCSSKEDSNKKNSTH"
     gene            complement(19121..20176)
                     /locus_tag="PC523_000403"
     CDS             complement(19121..20176)
                     /locus_tag="PC523_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2473971.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKKENLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGLISSEKYNDEINKILDAINRFEAVYKLSKQWIERNKKKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGIDPSI
                     PKDTKFHMKLGSKKLNK"
     gene            complement(20224..21024)
                     /locus_tag="PC523_000404"
     CDS             complement(20224..21024)
                     /locus_tag="PC523_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2473972.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLSKEGNIFGPLLFLLLYNIPALALRIFGLKYGY
                     QVGVNSLDRIQREGLMDKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVAGIVLHAIGLL"
     gene            complement(21017..21808)
                     /locus_tag="PC523_000405"
     CDS             complement(21017..21808)
                     /locus_tag="PC523_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2473973.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMFDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGITGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQRAVFYAKQADFKRVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKIILDGLGVAGTLLPAVGFALLMDMLFSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLIEGEIDFE"
     gene            complement(21843..22265)
                     /locus_tag="PC523_000406"
     CDS             complement(21843..22265)
                     /locus_tag="PC523_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDF2473974.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTKDKSADDA
                     EKEIRSILLNNKDSEAFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIIKSGLEEAKNGIVYVNELLENQNKIKNII"
     gene            complement(22281..22760)
                     /locus_tag="PC523_000407"
     CDS             complement(22281..22760)
                     /locus_tag="PC523_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2473975.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVIAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDNDDINNFKEISDLGIDIEFRMLPRDKKKQLSNLIK"
     gene            complement(22892..25648)
                     /locus_tag="PC523_000408"
     CDS             complement(22892..25648)
                     /locus_tag="PC523_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2473976.1"
                     /translation="MRDEILSFVNECALFNNFDELKKESGIVTEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKSKINDVVGPTKKSIYESLDELKNECKLLKQEIV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YIPNIEERGSIEKRKIVEHFIYEESKVLGLPIEITQITLNTILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIVFSPKQFSIEM
                     SNGTNKRSIIYQSFIKCLDYFDRLQKNEITKERFSKKSSNLVTRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLIMNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNAII
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKSKINSTMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVLAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSGVVENEKIKSIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLPNDKKIALYVHLSCM
                     IERLVRQAEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQISISEIGYIYNIIY
                     GLPQ"
     gene            complement(26239..27042)
                     /gene="proC"
                     /locus_tag="PC523_000409"
     CDS             complement(26239..27042)
                     /gene="proC"
                     /locus_tag="PC523_000409"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDF2473977.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAKDITVSDLSQVALDKV
                     KEEFGVNTTTDSKVAVANSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKAMFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
     gene            complement(27367..29736)
                     /locus_tag="PC523_000410"
     CDS             complement(27367..29736)
                     /locus_tag="PC523_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDF2473978.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFKSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            complement(29741..30649)
                     /locus_tag="PC523_000411"
     CDS             complement(29741..30649)
                     /locus_tag="PC523_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDF2473979.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSRCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIDKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKYADLFLYDIKLIDEEKHIKFTGKSNDLILKNVKVLSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTEIGG"
     gene            complement(30994..32073)
                     /locus_tag="PC523_000412"
     CDS             complement(30994..32073)
                     /locus_tag="PC523_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF2473980.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVGKSTGSSTP
                     IIVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTNNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTTMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASKAEGLGFAIPINTAKSIVEEVIKTGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TLSKGASTKITVNRGGKAVTINVNF"
     gene            complement(32228..33577)
                     /locus_tag="PC523_000413"
     CDS             complement(32228..33577)
                     /locus_tag="PC523_000413"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDF2473981.1"
                     /translation="MRKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSAEKIKSDSDFLLVIGIGGSYLGSRAAIDMVSHSFRNR
                     LKKEQRNAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDSSKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKNNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITINSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            complement(33841..35469)
                     /locus_tag="PC523_000414"
     CDS             complement(33841..35469)
                     /locus_tag="PC523_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDF2473982.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPIALGVGIILYLFGNNPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPILGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPIVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNFIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            complement(35592..37196)
                     /locus_tag="PC523_000415"
     CDS             complement(35592..37196)
                     /locus_tag="PC523_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473983.1"
                     /translation="MLRISNVKLGIDKDISLLKDIVLKKLRIKEKNLIKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILKSKIKDVTKIKEIRYINVPMGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVVINDTEKIDTDTIILAVGHSARDTYEMLYSRGVKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASTSNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGNVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSVRNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            complement(37244..37561)
                     /locus_tag="PC523_000416"
     CDS             complement(37244..37561)
                     /locus_tag="PC523_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473984.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGNEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            complement(37566..37931)
                     /locus_tag="PC523_000417"
     CDS             complement(37566..37931)
                     /locus_tag="PC523_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473985.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            complement(38012..39049)
                     /locus_tag="PC523_000418"
     CDS             complement(38012..39049)
                     /locus_tag="PC523_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDF2473986.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSLFESYVSIYEVKEISAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKKERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            complement(39302..39886)
                     /locus_tag="PC523_000419"
     CDS             complement(39302..39886)
                     /locus_tag="PC523_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473987.1"
                     /translation="MRLNNREKNLLKILLILVIMVASDIVVNYLEENLTSLKNEKQNI
                     INKINNQDNYQIKGNKHIDDKYIESVELNENEELKNNFNNSFLDKKTNKDIVLLVENE
                     LKNIVNIEYINQESSLDNHNKKQVNLEIKVNGELENVLKFEDVMKKLRLNNKLEKIEI
                     NKLKVNNFDAQNILSEYKQEKDNIVECKMTVRVT"
     gene            complement(39901..40344)
                     /locus_tag="PC523_000420"
     CDS             complement(39901..40344)
                     /locus_tag="PC523_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2473988.1"
                     /translation="MKVDKTINFLNGFKKTKSCNKVYKIIIVLILTCILFKSFMYVKS
                     INELEDYISQNKLVDKSKHTISTTDKTREKFSKIKLNEIKKIYETIGINSITRIWIDK
                     NKLEIEGVCSDLDVLDNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            complement(40345..41217)
                     /locus_tag="PC523_000421"
     CDS             complement(40345..41217)
                     /locus_tag="PC523_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HDF2473989.1"
                     /translation="MATIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFNIK
                     NDSNSIEKIKYIEDVVRFVKEEIDKSKISYKNLYFNIQIQDIVIRNVEVLDTKKKRDI
                     LSMIEFEITQYIPINISNYSIKYKVINSINDKLNIQVILFPKSIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIEETYLLSKERK
                     SYEFIKRLLRDFKYIYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDTDLE
                     DNSINYISSIGMII"
     gene            complement(41271..41621)
                     /locus_tag="PC523_000422"
     CDS             complement(41271..41621)
                     /locus_tag="PC523_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDF2473990.1"
                     /translation="MINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKLNAD
                     YTNAANIVTAASMAINDDENIIDSLSVEALKEKGYLNTVPVPQSTSGKFELVINDNGT
                     DISVNINSKQFYPK"
     gene            complement(41675..42760)
                     /locus_tag="PC523_000423"
     CDS             complement(41675..42760)
                     /locus_tag="PC523_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDF2473991.1"
                     /translation="MRLSKNIFCNLEKFYLVLNRISDKEIRVICKEIGILMESGCEIT
                     KIFEIIESQSSKKVKNLLNIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKIKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTSNG
                     IEPPLITRVLMGISTVVTNNLAYLIFSFILFSIGSIYFFITNDNIKKLTNSLKFKVPF
                     VKRINQLVATTRFSRALSILISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANIFPKLFISMTNTGEESGKLDKSLDVINKFYENELNNKIEQIMKFIEPV
                     LIVFMGLIIGIFVLAMVVPMFDAITSF"
     gene            complement(42931..44310)
                     /locus_tag="PC523_000424"
     CDS             complement(42931..44310)
                     /locus_tag="PC523_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDF2473992.1"
                     /translation="MIKIILEENSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVVKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDDFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            complement(44303..44983)
                     /locus_tag="PC523_000425"
     CDS             complement(44303..44983)
                     /locus_tag="PC523_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2473993.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKGKLDKEIDSMKKNQKNSEKISQIETLRDSLSYSDMKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKLNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            complement(45586..46665)
                     /locus_tag="PC523_000426"
     CDS             complement(45586..46665)
                     /locus_tag="PC523_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2473994.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNRQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVNKVY
                     YLAGEIGTGKTTLLNKVYNKAIEKGLNVEVYHYPLIPEKIETILLTDLDIGITISTNF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDNLVKRILKYEENL"
     regulatory      46951..47066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            47241..48611
                     /locus_tag="PC523_000427"
     CDS             47241..48611
                     /locus_tag="PC523_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2473995.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPTNEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQSKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGIIALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGSVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATVRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNKSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     gene            complement(48875..49486)
                     /locus_tag="PC523_000428"
     CDS             complement(48875..49486)
                     /locus_tag="PC523_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDF2473996.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(49476..51839)
                     /gene="lon"
                     /locus_tag="PC523_000429"
     CDS             complement(49476..51839)
                     /gene="lon"
                     /locus_tag="PC523_000429"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDF2473997.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGKVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLGNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVISKSDLEAYLGKDMFKYQLAEVNPQIGLVNGLAWTAVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPDFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            52019..52531
                     /locus_tag="PC523_000430"
     CDS             52019..52531
                     /locus_tag="PC523_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2473998.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            52519..53046
                     /locus_tag="PC523_000431"
     CDS             52519..53046
                     /locus_tag="PC523_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2473999.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATVGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(53115..54365)
                     /gene="clpX"
                     /locus_tag="PC523_000432"
     CDS             complement(53115..54365)
                     /gene="clpX"
                     /locus_tag="PC523_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDF2474000.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(54384..54968)
                     /gene="clpP"
                     /locus_tag="PC523_000433"
     CDS             complement(54384..54968)
                     /gene="clpP"
                     /locus_tag="PC523_000433"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDF2474001.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            complement(55118..56404)
                     /locus_tag="PC523_000434"
     CDS             complement(55118..56404)
                     /locus_tag="PC523_000434"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDF2474002.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            complement(56598..57068)
                     /locus_tag="PC523_000435"
     CDS             complement(56598..57068)
                     /locus_tag="PC523_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2474003.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTHMLEKKATEIDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDNINIEEIRI"
     gene            complement(57068..58417)
                     /gene="rph"
                     /locus_tag="PC523_000436"
     CDS             complement(57068..58417)
                     /gene="rph"
                     /locus_tag="PC523_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDF2474004.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTVKEMPITNFV
                     SAISVGIVNGTHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRTDLNLL
                     LELGEKGNKELIRLQKEALGEIADEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIDIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            complement(58576..60600)
                     /gene="ligA"
                     /locus_tag="PC523_000437"
     CDS             complement(58576..60600)
                     /gene="ligA"
                     /locus_tag="PC523_000437"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDF2474005.1"
                     /translation="MSIKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEDYDLESHSDSLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLEYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVELERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDDVMSATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLAKANDLGIKVIDEERFLELLKLSSKDEVKAILES"
     gene            complement(60787..61725)
                     /locus_tag="PC523_000438"
     CDS             complement(60787..61725)
                     /locus_tag="PC523_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDF2474006.1"
                     /translation="MKVLFTINYGKEKFDKIRDLGYDVIHYSEDIIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGLE
                     IWGVNTAGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECIEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYILENKPVDNVVDISRGY"
     gene            complement(61827..62249)
                     /locus_tag="PC523_000439"
     CDS             complement(61827..62249)
                     /locus_tag="PC523_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HDF2474007.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGDNIIRDILDGNISSECENGIKSKSVIFNNAPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            62437..63015
                     /locus_tag="PC523_000440"
                     /pseudo
     CDS             62437..63015
                     /locus_tag="PC523_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            63342..63614
                     /locus_tag="PC523_000441"
     CDS             63342..63614
                     /locus_tag="PC523_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDF2474008.1"
                     /translation="MQYLLIIIIGLLLFIEVDKLKKQVKSQQKQIDTLCEQTGNNQLA
                     TYHISDEDKELILHLKNSGKDVEAVKKVREITAMELEEAKQYVDSL"
     gene            63649..63867
                     /locus_tag="PC523_000442"
     CDS             63649..63867
                     /locus_tag="PC523_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HDF2474009.1"
                     /translation="MDNGGVQMDKRHWFLLGYLIIPVCFFVAVIVVGLLRSHSLIEIY
                     NDGLGITALYYLLLSLFVYIRWQYFSDK"
     gene            63888..64073
                     /locus_tag="PC523_000443"
     CDS             63888..64073
                     /locus_tag="PC523_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HDF2474010.1"
                     /translation="MYIIYTVLAAIVGMALVVTVDMGITKIFDKKSIFQKNYIKMPLS
                     YFIGIFVFLLIVRLIFS"
     gene            complement(64139..64447)
                     /locus_tag="PC523_000444"
     CDS             complement(64139..64447)
                     /locus_tag="PC523_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474011.1"
                     /translation="MKKKLLFATIILVLLAGILYYISLPDYLVFNSMSFSNGANRDTE
                     LQVIVYQYWNTDEVVAEIEAEHNQINGTPTTLIINLYHSKWSFHNGYEPFYSTIINYD
                     "
     gene            64561..64776
                     /locus_tag="PC523_000445"
     CDS             64561..64776
                     /locus_tag="PC523_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2474012.1"
                     /translation="MKYGHLELHIEELLKEKGISKNTICKELDIPRSNFNRYCRNEFQ
                     RLDAQLICKLCSYLDCGIEELIEYVKE"
     gene            complement(64969..65652)
                     /locus_tag="PC523_000446"
     CDS             complement(64969..65652)
                     /locus_tag="PC523_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2474013.1"
                     /translation="MNTIAERIKFAMKAKNKKQVDIVKDTGISKGAFSSYLSGQYNPK
                     ADKMELIADSLDVDLRWLYGQNVPMEHTSQNDNALQYVFYNNSCSEYLLDDLDDIYIA
                     MMTQYAALIPRFYVLVNRAGNAMHILPLFLKEDSSQFYECPSDFFYSDRHTIFTRDFE
                     SIHMVLTTATIYYYGIDTKTYEPKVTMLSYSQADDCFHINNGIQGGHIKAFEKELMKE
                     ALYLKHNDQ"
     gene            65901..66230
                     /locus_tag="PC523_000447"
     CDS             65901..66230
                     /locus_tag="PC523_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474014.1"
                     /translation="MQITLTKEQVKEAFGELRFMGADNCYRYDKDSKKKTEEVEALKL
                     HLGSMKLGNSVDIRLEATELPKIEPYAVVELEESVYAPYVQRGNFPVLVEKITCKGIR
                     PVPNKNM"
     gene            66289..67398
                     /locus_tag="PC523_000448"
     CDS             66289..67398
                     /locus_tag="PC523_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDF2474015.1"
                     /translation="MATPLQIGGMGNLEERSGSFLPELPYTNRGVIRQKEALSALIDW
                     CQITIKEVPLEAVIEDVLRIPLELMTVTGYEKGIAGHEVVAIFDNIKVLKPTGNAQYQ
                     GFQILMSGKGCRNYENFLQLNEETWFDFLNRVCQYHINVPRIDLAIDDRKPYLSIPDL
                     IVRTKEGLLSTKLREIDFHDSGELKEEVFQSKGGSLYLGSSASNLRLVFYEKGYEQNK
                     KYGTELDENWNRYELRFRQEMAVSVVQELLRYRDVAGLAMEVLNSKMRFLEKPTDSMT
                     TRKRLYPTYQAWAELMKDIGKVKLTMQPQKKSLQKVWKWLEKYVAPSLKLFAEVGKIE
                     QRDYIGNLVKNGEMNIAQKQLYDDYIKARKLDERG"
     gene            67418..67600
                     /locus_tag="PC523_000449"
     CDS             67418..67600
                     /locus_tag="PC523_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2474016.1"
                     /translation="MNLKEVSEYLGIGETKTRELLRDRNCPFSMKIGYRWYANKKILD
                     KWVQEQSEKKGGNYSI"
     gene            67686..68960
                     /locus_tag="PC523_000450"
     CDS             67686..68960
                     /locus_tag="PC523_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2474017.1"
                     /translation="MGKSLKGKELGAGLYQRKDGKYCGRFYDRFGIRRYVYGKTISET
                     KEKLNTAMYEDKMEMNVADNTVTLDEWYQKWLEIYKYKVIAPSTRVQYESIYKKHIQT
                     DFGHMKLKDIQPLQIRGKLVNLDKKGFGYETQNKVRILLLDMFNKAVADNVAMKNPVR
                     NIKVVRDEEKEPRVLSTEEQSAFFDTCKGTFYDNLFNVAISTGLRPGELYALEAEDLD
                     FEKKVIHITKSLSYQKFEGDTGKTFHMGKTKTKSSYRDVPMNRRCEIALKKQLLQKRV
                     VSGRNGAKTLKGFENLIFTTKYDTPLNAQIYADAIKVIVEQINLMREELEQFETFSGH
                     CFRHTFATRCFEAGIQPKTVQKYLGHATLQMTMDLYTHVLENHLSDEMDKLDSRLEMI
                     ELSSDDLAEQRFEREYRKQNEVLSSDKLIRIG"
     gene            69069..69779
                     /locus_tag="PC523_000451"
                     /pseudo
     CDS             69069..69779
                     /locus_tag="PC523_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(69855..70370)
                     /locus_tag="PC523_000452"
     CDS             complement(69855..70370)
                     /locus_tag="PC523_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2474018.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(70370..71746)
                     /locus_tag="PC523_000453"
     CDS             complement(70370..71746)
                     /locus_tag="PC523_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HDF2474019.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            complement(71771..72322)
                     /locus_tag="PC523_000454"
     CDS             complement(71771..72322)
                     /locus_tag="PC523_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2474020.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIIKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYQDGKEVEQVILDENKKIAYKCDLCKDSEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            complement(72543..73376)
                     /gene="fdhD"
                     /locus_tag="PC523_000455"
     CDS             complement(72543..73376)
                     /gene="fdhD"
                     /locus_tag="PC523_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDF2474021.1"
                     /translation="MNNIYSKTKLQQNSKDKVFENSYSLVEHKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNNKYINTFLCTPSNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIHEKQKNQNEQIIFLNSLDYITCAPVESNIKINYDTIYSIMDRNLNS
                     SELFKNTGGVHSVSIFNFDKRIVTCEDVARHNAMDKAIGFCVLNDIALKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            complement(73425..75569)
                     /gene="fdhF"
                     /locus_tag="PC523_000456"
     CDS             complement(73425..75569)
                     /gene="fdhF"
                     /locus_tag="PC523_000456"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(75153..75155),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDF2474022.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYAASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPDLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIEAQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAKKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYKSLKDKMTATNI"
     gene            complement(75820..76878)
                     /locus_tag="PC523_000457"
     CDS             complement(75820..76878)
                     /locus_tag="PC523_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2474023.1"
                     /translation="MDRLKSIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPQKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            complement(77089..77748)
                     /gene="cprR"
                     /locus_tag="PC523_000458"
     CDS             complement(77089..77748)
                     /gene="cprR"
                     /locus_tag="PC523_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HDF2474024.1"
                     /translation="MSKILVIDDELDVLKLIENVLKRDGHEVKVISDIENAMDIEFNS
                     FNLIILDVMMPKMDGFEVCKAIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVLIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHVKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            78058..78687
                     /locus_tag="PC523_000459"
     CDS             78058..78687
                     /locus_tag="PC523_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HDF2474025.1"
                     /translation="MDEKIRSSNYNVPFDYARKKFKELDPHTISNLSNISFNNDSNKF
                     ILNFFDRQYIIDYPSGEVWNDDGSICKNFELKILIIRYLTNAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLSRLSEVFTCQIDKYKLVMENMGAEKVDMGDLAYKFTYMGNTSMI
                     FILWFGDDEFPPNSNILFDSNIIYYFNAEDLAVVPDTAIDAILNKINQL"
BASE COUNT        24930 a        13732 c         9103 g        31101 t
ORIGIN      
        1 aaagtatacc tcttttttgt attttttata ttatatgttt tactttataa ataatttact
       61 ttatttttta ccatatttaa atttattata agttttcttg ccagaaattt ttaggtgata
      121 tattttctgt aattggcaat attgtatatc ctctctcttt taagattctt ataatattat
      181 ttaaagcatt taaactttgc tctttctcat gaattaatac tactaattca tctctttttc
      241 ttccataata taatatatta cttactatct gacttgtaga agacttccaa tcctgtgtat
      301 ccaaattcca gtcccaaatt aaataatcat ttgccataag tttatcatat gaaccttctg
      361 gcatatatgg tttgcttcca tatggtaatc ttattaattt tgaactttga ccagttattt
      421 cttgtagagt attattgcat gtattaaatt ccccaactgt tacttctggg cttttatata
      481 acttatgtat atcatgactt acactatgaa aacctattga attttcctct tccaaaactc
      541 ttcttacttc ttccttgtga tttttcatat tttgattaag catgaaaaat gtcgctttga
      601 catcattttc ttttaaaata tcaagcagtg catttgtata ttttgatgga ccatcatcta
      661 ttgttatgta tgctactttc tcagaagtag ctcctggacc ttttgtttct tcttgagatg
      721 ctttatatat gatttctcct aaagtattac tttgagaaag taattccagt gtactttcac
      781 cttttgaact tatattttca actggaacat tattaatttt ttcctccaat aatgttccag
      841 ttaaaattaa aacagctaca agaaatattc ttacaaatac atgaaaaaca gtcttattat
      901 ctttaatttt catcccccat atctcccttt tataaaagca taagtttaag acttaaaaag
      961 ttatttttta tatttattct ctttcctatt actttttata tctctactaa atattttttt
     1021 aaccttttct tcttcttttt ctcctagaga ttccactctt ttgattccca aagaagttaa
     1081 aaattgtaat acatttaaat tatcaaatgg agtttccata ttcgtacttt cactatattc
     1141 atcaacattc ttagtacctt cttccacact taaaatacgc ttaggacctc ctacacagcc
     1201 tcctacacac cccattcctt ctataaatgt agcgtctatt tctttattta gaactttctt
     1261 tagaccgtcc atgcactctt ttgttccttc aaatgctttt tcttcaaatt ttatattttt
     1321 gtcaattctt ctaactgata acttaactgc tttacttaca cctcctgttc tagcataaac
     1381 tctacctgaa agggatgcct ctactctatt actctcatcc attattgctg gatttaactc
     1441 taatgcatca aatatttctt gtaactcttt aaatgtaagc acaaaatcaa ctgcatcttt
     1501 tatgtcttct aacattgctt ctttcttttt tgccatacaa ggacctatga atacaacttt
     1561 agcatctgga ttcaaaattt ttacagctct cccacaagca atcataggag aaacggatgg
     1621 tgatacattc tccaatatct gaggaaagct attttgtatc aaactaaccc aaataggaca
     1681 gcaacaacta gttataaagc atccttcttc gtcctttttc atatgttcat aataatcata
     1741 tgcttctttt gcagtaagca tatctgctgc caaagcaact tctatcatat cttcaaatcc
     1801 tatcttcttt aatgcagttc ttaatttacc agggcttaca tctttcccaa actgtcctat
     1861 aaaagctggt gcaactattg catatacagg gctctttata tcttttaaca attttatcat
     1921 tggtataaat tctattttat ctgaaattgc acccaactta catcttgata cacaatcacc
     1981 acaagcacta catacatctt cctcattaca attaatatgc ttacttctta tcttacatga
     2041 ctcatcttcc ttattatttt catcacaata tttgcaagca tctgatatta tggatattat
     2101 tggtcttttg acttcatcta aatttacagc ttcatgtagt gaatctttta aatttttatc
     2161 tgtatctcct gtcacatcca atcccatagt tactctaatt aaatttacca tagtatcttt
     2221 atcgaatttt ccactaaaat aattatttaa atcatctttt aaatcttcat ttaaaatttt
     2281 attaggtaag ttttcaatat cctctatatc atcattccac actgctttta caagttcgcc
     2341 aaaaagcttc attttatata tttctaatat tttagaatta tcatttttag tattcataaa
     2401 aaatagtacc tccataaatt aaacttatat atgtttaata tttacaaaga tactattttt
     2461 tatttattat ttatctacta ttattaatat gatttattta actttctcag caattctatt
     2521 tcaatcatta ttttcattag atgatagttg aatacaggac ttttttcttc atctatattc
     2581 cagtctagct tatctacatt atatgttttc ttaaggtatt tatatgtatg ctctttcaaa
     2641 taaagcttat tttcaagtaa ctctatagac tgcatatgaa aatgtatttc tctacatatc
     2701 cttaaggcgt tctcaatttt ttctatatta gtttcactca aactataatc caactcatca
     2761 ataagttttg aatatatttc ttctagtttt tctacttctt cttcaaactt ctctacattt
     2821 aaattttctt ttctcataat tttatcttca ataagcaaca tcgaaagttc ttctatcttt
     2881 ttaaattcat ctatgacgct atttagataa tttggtctag caacataata attaacaaga
     2941 accccaaaca aaacacccac agatgtatct aacaccctat gtaaagaata atacacaggg
     3001 tcactatttc ctacacctaa atgtatagcc agaaaggtta cacaggcaac tactactgac
     3061 tttattttaa acagattgca taaatatatt gagcacatta ctcctagacc acataaaaaa
     3121 gggtctccag ctttaatgat ggcaaatatg aatcctatta taccacctaa aatagtacct
     3181 tccactctat tgattcctgt ctttaaagaa ccttttaccg tatcttgaac agaaattata
     3241 catgcaacta aagaataaaa catattctct actagatagt caccaagaat catacataaa
     3301 gtgacactaa ttcctgtttt tatagtcctc atacccatct tttgaagttt caaaattcca
     3361 cctcctaaaa agcaccttat ataaaatacg catcctcata tttaatatgt tcctagtata
     3421 tcacaatttt ttaaatttac ccattaatca tattttttta tcaattcttt cttaatttag
     3481 acaaaaaaaa actgctttaa agcagttttt ttatcctatc tcaaccattt taccatcaat
     3541 tgcaaaaatt atcttctcgc atacatttgt tatatggtct ccaattcttt caaggtatct
     3601 tcctataagt agaagtttta ccccttggtc tatagtattt gaattttcat gcattatttt
     3661 taaacaatca atttgtactt gttcatacaa tccatctaca atatcatctt tagttccaac
     3721 atcataagct atacttgcat caaataagat taaagcttct ctagtctgag acatcataga
     3781 cttacattct tttgccatct taggaatatc tatcagctct tttatataag cctctccacc
     3841 tatcttcaac atttctctag ctatatttgc tgcataatct cctattcttt ctaactctat
     3901 cgatatatta cctaatgaat aaataaatct taagtccctt gccattggtt gctttaatac
     3961 tattaattct atactcttat ctcttatttc ttctcttaat ttatctattt cttcatcatc
     4021 ttctactact tttcttgcac cctctaaatc cttctctatc atacaagtca cagacttttc
     4081 tattgcttct tcacattttt ctatcataag tactgtttgt tcttttaaag tatcaatact
     4141 tatatctaaa ctattattga ccattgtcta actcctttat agttatatat tttaaccaaa
     4201 tctacctgtt atatagtctt ctgttctttt atctacagga gtggtaaata tagtttttgt
     4261 atcgctaaat tctataacct ctccatttag gaaaaaagct gtttcatctg atatacgagc
     4321 agcttgttgc atattatgtg taactatgac tattgtgtag tcttttttta aatcctctat
     4381 aagttcttct acttttaagg ttgaaattgg gtctaaagca gatgtaggtt catccataag
     4441 aataacttct ggtctcatag caattgctct tgctatacat atacgttgtt gttgaccacc
     4501 agaaagtcct aatgcagatg atttaagcct gtctttaacc tcatcccaaa tagctgcacc
     4561 ttttaagctt tcttctacaa tcttatctaa ttgcttctta tctcttagcc catgtatacg
     4621 aggaccatat gccacattat catatatact cataggaaat ggatttggct tttgaaatac
     4681 cattcctact ttagtacgaa gatttataac atcatctgaa gtatatatat cctctccatc
     4741 aacagaaata cttcctttaa ttgttacatc ttcgattaga tcattcattc tatttaaagt
     4801 tcttataaaa gttgattttc cgcatccaga tgggcctatt aaagcagtaa ccttattctc
     4861 ttttatatcc atatttattt tctttaatgc ttgcttatca ccataaaata aatccaaatc
     4921 ttttacactc atttttatct tatcgattaa ttccattatt cacacctctt aaatttaata
     4981 attagcctta tttaatttct tagcaacgaa tcttgccaac atgtttagtg ctaatacaat
     5041 tactattaaa acaattccta tactggctgc tgttgctata tctccactct ctttagtcag
     5101 taagtatgag tgtacagtta atgttcttgc actatcaaat attccacctg gcatcttagc
     5161 tacagtaccg gctgtaagta atattgcagc tgattctcca attatacgac caattgataa
     5221 gattactcca gataagattc ctggtattgc acttggaagt actaccttat ataaagtttg
     5281 aaattttgta gctcctaatg ctaatgatga ttctctataa gcttgaggaa ctgtttttaa
     5341 agcttcttct gttgtacgaa ttattactgg taatattatt attgctactg taagtgaacc
     5401 tgcaactatg gaatatccca tttttaatgt aactacaaag aaaattcctc caaataatcc
     5461 atatactata gatggtatcc ctgataaact ttcagttgca aatcttatag ctgtaactgc
     5521 ttttcctttt ttagcatatt cttgtaaata tatagcagcc aatattccta taggagttgc
     5581 tacaactata gaaactaata ctgtatacaa tgttgtaatt atcattggaa gtataccacc
     5641 atcacctgat gctgaataat tgctaaataa gaaattaaaa ttcatttttc caattccctt
     5701 tataaatata aatcccacta taactactaa tgatactatt gtaaatagtg ctgatagata
     5761 tactaatgac ttcaatagat tctctttgaa ctttctcatc ttaatttacc gccttatttg
     5821 aaagtttatt taaaactaaa tttaaaatta atataaatgt aaacaatact actccagttg
     5881 caaatagcat ttcttgatgt gtaccaaatg cgtaacccat ttcaagtgct atattagttg
     5941 taagtggtct tacactatct gttattgcag ttggcataac tggtgaattt cctgcaacaa
     6001 gtattaccgc cattgtttct cctaaagctc ttcctactcc taacactact gcagctaata
     6061 ctccagattt tgcagcaggt aatacaactt tgaatattgt ttctattttt gatgcaccaa
     6121 gtgctaatga accttcttta tatgcttttg gtactgctct tattgctgtt tctgatacag
     6181 atattattgt tggtaacatc attactgcta atactattac tattgctaac aaactttgac
     6241 cttttggaag attaaatata ctttgtatca ttggaactat cactgcaaga ccaaatacac
     6301 catacaatac tgaaggtata cctgctaaaa gttcaactgc tggtgacatt atttttgcta
     6361 atcttttagg tgctacttct gctataaata cagaggttaa tataccaaca ggtactccta
     6421 tcacaagtgc tccaagtgtc gcaaatattg aagctactat catagaagat attccaaatt
     6481 tattggcaga aggaacccaa tttgcaccag ttaaaaagtc tataaatgaa tatccttcaa
     6541 atataaatgg tcttaatcct ttataaaata cgaatcctat tattaaaagt aaacttgcaa
     6601 ctgctactaa agcactaagt agaaaaatat tttttgctaa cttttcaatt acgtattttg
     6661 tcttattgcc attattgtca tttttttcta aacctactaa tctagacttt actatcataa
     6721 tatcctccca ttaaaatata cttatcgatt ttcattctgt attcaaaatt taaattttta
     6781 caattcgtct tcatcaaatt tcctgtactt ttattctact ttttttaagt taaatctata
     6841 ttaggattaa gttaaacaag tgtaaaatta tattcacttt tttaacatta attttacttt
     6901 taaaactata tttgacattt actttacata taatggtgtt ttgctatatt aaaatagcag
     6961 atataggtaa taatacgctt tatgtgtaat ttattaccta tatctgctat taagatttgt
     7021 taactaaatt taacttttat actacatata taattgttat ctcctattgt ctatatcact
     7081 attattttca taaaattgtt atgatacttg atgtttacaa taagtacgaa tagacaaaaa
     7141 tataaaaata ggttttatta ggcttatgat aaaaaacaac atttgaaatg atattttcaa
     7201 acattgagca ccaaaagtta aatcataaat tgccaaataa atagaaatac atacaacaaa
     7261 cattactata aatatagcaa atagagctaa tattttagta aatgctaaga taagtgtatc
     7321 catgtttagt acaatgaagt atgctatatg atagtgtctg atattcactt cacaaaaaaa
     7381 ggtagatgca aagattgcaa cacatacaat taataatatg aatatattaa ggataaaaat
     7441 gaataatata tagatttata tatacaattt gtttatcttt tcaaatgtaa cccataaaat
     7501 aatggtgcag gtaaacatct ttttaagtaa taaaactgac tgtaatttaa ctgataaagt
     7561 ctagtaaata ggctttatca attaaattac agtcagctat tttataataa tttagtttag
     7621 atgtcgttaa aatttcacat ttataacaag tattaatata tttattattc cttattaaaa
     7681 taatatcctg ctcctcttat tgttataatt ttttgaggat ttttataatc tttctctatt
     7741 ttctttctta atccattcac tgcaactacc acactatagt tatcctctaa atactcattc
     7801 atccatacat gttcatatat ctctttagta gtcaaagtct tttcaggatt ctctataaaa
     7861 aagtttaaca acttattttc tgttggagtc aattttatta ttttatcttc taactttatt
     7921 actccactag ttctattata ttcctctaat tttgtattat tttcattatt atttacatat
     7981 gagttcattc ttcttaaaat agcttttact tttgcatata atatttctaa atttagaggc
     8041 tttattaagt aatcatctcc accagaattt aaggcattta aaatgctttg gtcttcattt
     8101 atgtaagtaa tataaacaat tggacaagta gttacatttc taattttttt acaaagagtc
     8161 cacccatcac catcagttag gattatttct aaaaatatta aatcatattt attgctaaat
     8221 atttttccaa tagcttcttc acagttatat gctaaatcta tacttattcc tttttcttcc
     8281 ataaattttt ttaatttctg gcaattatcc ttgtctccat ctattatcaa tatcttagtt
     8341 tgcattataa tcactcctaa atatcattat tcttttatat taaataagct attattataa
     8401 aaaacataga cttaatttta ttgtaaaaac agttcctttt cctagttccg aatctacaaa
     8461 gatttctcca ccatgctctc ttataatgga tttgacaata tataacccta acccttgccc
     8521 ttttggatta ttgttatctc tttctgtaaa gaatctatca aatacctttg gtatattttc
     8581 tggaggtatc ccttttccat tatcagaaat tttaataatt gcaaatccat cttcttcatc
     8641 cattgacaaa attatcttcc catcaaatgg agtaaaggtt aaagcattat aaattaaatt
     8701 ctcaaacgct cttataagca tctctttatt tatctttgca tatatttttt tctccaatat
     8761 gtaagtttca aagtttacac cacatgcttg tgtatctggc aagttatttt catatatctc
     8821 tttaataaaa tcattcagac atattttttc actaatacca atacttttat cttgtgatgt
     8881 aaattcttgc aaactacgta ctctattttg tacttcattt gacttttgat ttatagcata
     8941 aaggtaggtt cttgtctctt catctacatg aatattacca acctttatca agtctataaa
     9001 tgcttgtata gccgaaacag gtgcctttaa atcatgtgct acatctgcca aaaatttttt
     9061 acgttcattt aacaatgttg taagttcatt agttctcact tcaacttcat gttcaagatt
     9121 tcttgtaagt ttttcattct cttttattat tattctattc ttatttatca tcataacaga
     9181 aaataaaata acaattataa acccacaata ttctgtttgc caccctgtaa atataggttc
     9241 aaacctgttg ctagtaattg catcaaataa tatgctaatt ccaaaaacag tatttactga
     9301 aactaaaata taagaactta tatgttcaga gcatgtcttt ttatatattg aaaccagtga
     9361 agacattaat atatatataa atacaatata tttatatatg tctattaatt tcccatatat
     9421 gttgacaaag tacatatcat atggaagaag aataattggt atcactatag acatgatact
     9481 cattgtaatt cctaatggta aaattaaaaa tctatacaat ttattttctt ctattttgga
     9541 tatacttcca tttatacaaa taatacataa aactgctata aaatatgatg aatcttcaac
     9601 tgcatatgta aagtttacta aaggtagtcc taacaaatgt acaaagggat aagatacatg
     9661 tatggcaaag aaaatacaca tacacccaaa atataagtaa agtttacttt tgtttgaaag
     9721 aaaccataca gacaacgaaa atatagctat tgcaaaagtt gtgaaacaaa ttatactata
     9781 aaaaagtact ctataaaaca tcatattact tatatccttt gtttctccta atgcaggtgg
     9841 ataatacaaa ccgctataat aatgtgaaaa attcgataca ttgattataa tttctgtgtc
     9901 tttctttgca ataaaactta ctactaaatt tttaattttt ggtctatatt tttctgttcc
     9961 tacatctcct aatttagaaa ccatttctcc atttatccat acttcacttg cagaaaaaat
    10021 ctcaggaagc tccaaactta gtaacctatt cttaccatca ttttttattc tcatacgata
    10081 agtagcattc ccatatggtg attgattccc ttttctcata gaaaaatctg gatattgacc
    10141 tataaaaaca tattctggtt ttatatcttg ctctataaaa tcattcggct taaatatttt
    10201 atgattataa aactcccagt catctatcaa aaatacaagt ttacttttag tgaaagtctc
    10261 attatctata tttaaaattc cattacttgc tctaatagac ttgtgcgtat atttgttatc
    10321 atatttatac agaagattta atcctgttat tgccaacaga gagactatca atacaatgag
    10381 agttcctttt agtatattat actttttcat tgcatcaacc acatctcttt catagtagtg
    10441 ttaaataaaa taagaactat ttattaatta cagcatctca cctatggtac aaacttatat
    10501 ccttttcccc atacagtttg aatatattga tgctccctgc ttaaagaatt aatttttttt
    10561 ctcatctgtg caatatgaac ctgtactgta tgttgatccc caatatcagg cattttccac
    10621 acttggttat atatttctat cttcgagaat acttgctctt tgttttcagc tagaaacaac
    10681 aatatttcaa attccatagt tgttaaatct atactttctc catcaaaagt tacttttctt
    10741 gaatttacat ttattactaa cgctgaaaac tttaacacat taggaactga tgtaatattt
    10801 ttgtactgcc tcatacgagc atatattcta agttcaagtt cttttaaact ataaggtttt
    10861 gttatataat catctcctcc agctaaaaat ccccgaactt tttcttcttc atgctcataa
    10921 ttagataaaa atattatagg tatgttaacc ttttctttta tatcattgca cattttatat
    10981 ccatctatat ctggcaatga aatatccaaa attatacaat ctaaaactat actctctact
    11041 atatccatag catctttagc agtctctgat gtatatacat taaaacctat attagaaaaa
    11101 taaatttgat ttatgtttaa aatatcaaca tcatcatcaa caaccagtaa attatacatt
    11161 acaccactcc attcaaaggt attttaatca tagtctcaag ttttataatt attacaaatt
    11221 ataatgtttt attttcatat ataactagta ttattatttt ataatatctt tacaaactag
    11281 actataatac ttaagtattt ttgaataatt cttgaatatt gtattaaaca ttaagactca
    11341 ttattaaata ctaattaaag tctccgattg ttggagaatg gagattaaag tcatttttac
    11401 ttataaattt attatttttc tagaaacttg tatattggct agtttgagta ttgatttata
    11461 tgatttatat ggttataata taaaataaat tgtattattt tggtaaatat attgttacaa
    11521 aaggtttata ttctgcatct caatgtttat aaaaaagtta ttttcttatt ataatagcat
    11581 tatacttttt aaaaaatttc ccttttccaa caaatggcta ctttaaaagc caaaatttca
    11641 cctatcaata aaaatcttaa cgacgaacac tctttttttc aaatttttca acatttttgt
    11701 taattttaaa acaaaaaaag actatgcaat ataaaaatta tattcatagc cggttgcacc
    11761 actaactcca tatatctcta gatgcccaat aataagatat acttctcagt ttctaaaata
    11821 tttttattaa aaatctttta tatattttca taaatttctt ttttattcta acattgtaat
    11881 attattttgt caatatttcc atatttttag atataaaata ctaaaatata tattttatct
    11941 aaagatatct ttatttttac attcaagaat agacaatacc ctgcaagtta aagcatcatt
    12001 tttatgagct tatctcacag ggtattatac atctacttta tattataatt tatgttattg
    12061 attaacttta atgaatcctt tttctgaaac aattgcttgt ccttcatcac ttaatatata
    12121 atctattaaa gctttacctt tttctgataa accgtcttct ttattaacta atataaatgg
    12181 tcttgatatt ggatatttat tagctattac gtttgcatca gttgcttcaa catcatttat
    12241 ttttaagcta tttactttct catcaacata accaaatgat atatatccta tcgcattttc
    12301 gtttccttca actgtagttt ttatattccc tgaaccatca cttatttgtg catctttagt
    12361 taattcttct gagtcaaaac ctacaatttc ttggaatcca tctctagttc ctgaaccttc
    12421 ttctcttgat actattacta ttggtgcatc atttcctcct acttctttcc aattagttat
    12481 ctttcctgta tatatacctt taacttggtc cattgttaga ttttttattg ggttagcagt
    12541 attagttatc aatgctatac catctattgc tattttagtt tcctttaatc cagcactttt
    12601 ttcttcatct tttaaatctc ttgaactcat tcctatttca gatgttcctt ctatagcatt
    12661 ttttatacct ccagaagaac caatttgttg tacttctaca ctaacacctt tatttttttc
    12721 ctgaaacttt tctccgatag tttccataag aggacctatt gaagttgaac ctgaaacaga
    12781 aactttactt gaatctcctg aatttcctga atttcctgaa cctcctgagt tagaaccaca
    12841 ccctactgct aaagctccaa ctaaaattgt actagctaaa agaactccta ttttcttttt
    12901 aaacatttta cttacccccg cttatatttt aattttttgt tgtcattttt ctatatctta
    12961 ataatacaga ttttttgtta aatgaatatt agagcagggt taagtaaatg taaatattgt
    13021 taaatacatg taaaaactta attttatata atttattcct aattagatta actttttaaa
    13081 gttagtttta aatagatata aacataatac aactcatatt attataattc ttcattgaaa
    13141 ctatcaacct attacattct ataactcttt ttctagttta ttaactaatt cagcaaacac
    13201 attcaatgct tcctctatag attctttttt cgtcatatct acaccagcag attttaattg
    13261 ttctagtgga tattctgacc ctccactttt taagaagttt atatatctat ctactgcagt
    13321 acttccttct gaaagtatct gttgacttaa agcacttgca gcagaaaatc ctgtggcata
    13381 cttatataca tagaagtttg aataaaaatg agggattcta gcccattcta ttcctatttg
    13441 tttatctact attgcagatt ttccgtaata cttttcgttt aatttgtagt atatgtcagt
    13501 aaattcatca gcagtaagtg gttctccatt ctctactctt tcatgtacaa ttttctcaaa
    13561 ttcagcaaac atagtttgtc tataaactgt agttctaaat tgttctaaat aataatttaa
    13621 taagtatata gtttcatcct tagatttaga attttctaat aaataattta taagaagtaa
    13681 ttcatttaat gtagatgcaa cctctgcaac aaatatctta tagtctgaat ataaaaacgg
    13741 ttgacttgtt cttgaataat gactgtgaac agagtgccct aattcatgaa ttaaagtgaa
    13801 tagagaatta aggtcatttt tatagctcat taatatataa ggatgtgaat cataacttcc
    13861 ccaagaataa gctcctcctt ttttaccttc attatcatat acatcaatcc agccttcatc
    13921 aaaagcactt tttataacct tcaagtattc ttctcctaaa ggcttaagtg cttctaatat
    13981 gatatcttgt gctttatcat atggtatatc catgtcaaat ttatctgtca aaggaacata
    14041 caaatcatac atgtgtattt cattcaatcc taaaaatttc tttttaagct caacatattt
    14101 atttagatta ggaatacttt tatctatagc tgatataaga ttattgtaga catctaaact
    14161 tacatcatct gaaaataaag acgcttctat tgctgaatta tactttctag tttttgcgta
    14221 aaatatctct gattttattc caccataaag agttgaggca aatgtatttt tatatttctc
    14281 atatgttgaa aattcacctt caaaagcgtc ttttctaact ctactattct tactttttat
    14341 aaataaagaa tagtttgaat gagaaagctt gaccttatta ccatcttcat cttctatctc
    14401 aggaaattgc atatctgcat atgctaacat atcgtataca ttttctggaa ctgtcgataa
    14461 ttctgatgta gctgcaagaa tttcttcttc tcgttcactc aaagtatgtg gtttatctct
    14521 caatatatcc tttatatatt ttgtataaaa agataactta tcatctttta aatattcact
    14581 taacttattc tcttctatag ccagtatttc tggaactata taagaagtag ccacacccaa
    14641 atcagtagat aacatttctg tcttagtagc tcttccttga ttatcattaa ttctagtatc
    14701 ttcatgttgt ttcatatgag tatatacata taaattttga acaatcctag atgcattttc
    14761 agagatattt aatactttat ataagttctc cttactatca gaaagcttcc ctttatattc
    14821 ttttacttct tggataagat tttttacttt gcttatgtct ttttcaattt cctctttgtt
    14881 agagtacatc ttatctatct tccatttata cttttcttct attgtacttc tatcagttcc
    14941 catatcatac acctctattt taatttgttt tatattttat tcactttaca aaatatattg
    15001 tatatatttt agtccaaata atctacattt attactcctt ctatcatatt tatttctttc
    15061 ataacatctt gcaagtgtat tctcttagca taagatacaa aaaaattgac cttccagtat
    15121 tcttcattac gacattctat ttccatattt cttatgataa gttgactttt ttcaaattta
    15181 tcatataaaa aaggtataga gtccaaattg cttaatttta atatcaatac atttttttct
    15241 tgactaaata aaaatgtatt gattatccta agcacaaata atgctatcat tacaaataat
    15301 ccacctagaa ttgccaattc ataataacca tatccaactg ctacaccaat acatgcagtt
    15361 gcccacatac cagcagctgt agtgagacct tttactccac ctttattttt taaaattgct
    15421 cctgcaccta aaaaaccaat tccagataat acttgagcag gcattcttgc tgggtcagca
    15481 tttgtataca aattataatc aaagaataat tgaatcgaag ttattgaaac aatacaagaa
    15541 cctattgcaa ttagtatatg tgttctaaat cctgcaaact gtcttatttt ttctctctct
    15601 aaaccgatta ttcctccaat tattaatgct aatagtaacc ttaatgctat ctccttactt
    15661 cctatatcaa tcataatata aaaccctcct gacttaatta caacctatgt cctacagata
    15721 atttacacat cagaaatact attttatatc ttaatattat tattaatata tgtaaaatat
    15781 agccatctgt taatttttga tacaaaaaaa cccacaactg tgggctattt gcttaggtac
    15841 atgtatttat ttattaatat atctattttc ttacttgatt ctactaactc ttcttgaaat
    15901 atttctggat attctatgta tgtatttatc tcttccctaa ctgcttcaat ctcatttttt
    15961 aacgcttcta attcattgtt cgccataata atcaccacta ttacaaattt gtatattttt
    16021 atatacattt tatcaaacaa tattatattt tgtcaatatt atgtcaataa aacttactat
    16081 aaaaatgttt taatttaggt attgtatacc tggatactaa acctacaaac ataccagtta
    16141 ctaatgatgc cattaacata aatggtaagt accctatagc aagtatattt tctatgatta
    16201 aacttgcaac tattaattga cctatattat gccctattgc acctataatg cttatcccta
    16261 ttattgataa accttttatt ttgctcgcta aatacataaa taaaagactc agataagctc
    16321 ctcctatact aaatagtaga tatgataaag ggcctgcaaa tattgatgac aaaattaccc
    16381 tgatagtaac aattataaca gtatctttga cacctaacaa cattaatgaa attaaagtta
    16441 taatattgct caagcccaat ttcgcccctg gaaatataac tataagagga tttggtatat
    16501 atgtctctag tacatataaa gctaaactat accctatcat aagacctaaa aatgtcattt
    16561 ttctagtttt cattaagtta actcctccaa attttatcta tcatcttaca ttataataca
    16621 aaatatataa aaacacctaa tttagactaa aatatttttt aatctattta gaataaatat
    16681 tagttatttc aatgtgcaat tacctctata attcttgact ttttgtatca tacttgttac
    16741 aattttaatg ataatgaatt tcattaggag gtgttaataa tgactgttta cgatttaaaa
    16801 ataggagaaa aaggatttat taatagtata gatggagata ttaaattatc taaaagatta
    16861 cttgcacttg gatgtataaa tagtactgaa atagaagtta aaaaggttgc cccacttgga
    16921 gacccaatta taattagatt tagaggcttt gaccttgcga ttagaaaatc tgatgctaaa
    16981 aatatttctt tacaataata gaggaggagc tacccgtgat taatgtagca ttattaggaa
    17041 acccaaatgt cggtaaaact actgttttta atctacttac aggttcaaat caatatgtag
    17101 gtaactggcc tggtgttact atagaaaaaa aagaaggatt tcttggaaaa gagataaagg
    17161 ttgtcgattt acctggtata tatgcaatgg atactttctc aaatgaagaa aaggtttcta
    17221 aatcatattt agaaaatgaa gatgttgatg taatagtaaa tgtagttgat gcttctaatt
    17281 tatcaagaaa cctttattta acaactcaac ttatgcaatt taacaagcct atagttatat
    17341 tgcttaatat gttagacatt gcagaatcta aaggagttaa tatagatgct aaaaaattag
    17401 gcgaagaact tggagtaatt gttgttccaa ttatagcaaa aaagaaaaat ggcgttgata
    17461 ggatagaaga agagattaag ggagctacac aaaaagcttt tatatatcaa aaaaactttg
    17521 gaagtgaaac agaaacatat aaaaaattag aatccatttt atcaagatgt acaaaaacta
    17581 catatacaca gaaaaaatct atcagtgata agatagacaa tatagccttg aatcctatat
    17641 tagcatatcc gatatttatt ggtgtattat tcctcttatt taaatttacc tttgattggg
    17701 ttggaggacc gctacaagag ggatttgcta gtcttataga ggcttatatt tctgcacctg
    17761 ttagtgatat gttatctaac tctagcccat ggtttaaatc acttatagtt gatggaataa
    17821 ttggaggtgt tggtggtact ttaccattct tccctttaat atttacatta ttctttggta
    17881 tatcgttgtt tgaagatagt ggatacatgt caagaacagc atttttaatg gataaagtca
    17941 tgagaaaagt tggtctatct ggtaaagctt ttatacctat agttatgggt atgggatgtt
    18001 cttctccagc tataatggca actagaacat tggaaagtga aaaagataga agagttacag
    18061 ctcttattgc accacttatg acttgtggag caaagctacc tatatatgca ctatttgtag
    18121 ctatattctt tccacataat gctgctttag taactacgtc actgtactta gtaggtatag
    18181 ttatggcaat tgtagttgca ttattcttaa ataaaacagc cttcaaaaca gaggctgaac
    18241 catttatatt agaattacca gaatatagaa tccctactat agatgcactt attaaaaata
    18301 cttggaataa atcaaaaggt ttcttgataa gagttgttac tgtcatgttt gcaatgtcag
    18361 tcgtaatttg gggactttct tcattcaatg tttttggata tacagaggat ataaatgtaa
    18421 gcttcttggc tactctaggt catattatat ctcctatatt caaacctctt ggttttgatg
    18481 attggagaac ttcagttgct attttaagtg gtcttggagc aaaagaaatc gtcgtaaact
    18541 ctttaaatat actatatggt aatctaactg ttgtcttacc aacaatattt aatggagtta
    18601 ctgcatatac tttcttgata tttactgctt tatatactcc atgtatagct gcacttgcaa
    18661 ctatgaaaaa agaatatggc aataaaatga tgtttgcatc atttgcatat caatttatac
    18721 tagcatggat aatggctttt atagttaaaa gtataggtgg agtactattt atgggcaatt
    18781 caatagtgga agctttgata ggtggaataa ttatagccct tgcacttttt attttgttta
    18841 ataagttcaa gtctacaaaa aacggaagtg tgtgtagtgg atgttctatt tgctctggat
    18901 gtccaagtgc taattcttgc tcaagtaagg aagacagtaa taaaaaaaat agtacacact
    18961 aatatataaa tattttagtt taaaataacg tagaagtgag tgtcttaaaa ttaacttttt
    19021 agttcattag gcactctttt ttatatggaa taaaatatat ttttatcatt tcaacagtca
    19081 aaaagaggct tatctatatt ttgagacagc ctcttatttt ttacttattc aatttcttgc
    19141 tacctaactt catatggaac ttagtatctt ttgggatgct tgggtctatt ccccttaatg
    19201 taggtatttc cccacatatt agttgtatag gtactataaa attaaatgtt tgatagtaag
    19261 gattatctgt aatatctatt tttaaatttt tatcattttc tgtaacatct ctacctatag
    19321 caaatacatt ttccgtccaa ccagaaagta catcaatcat cttagttact ctaggttctt
    19381 ttcctgtatc tagtataaat atagtagaat cactatttat agcattatat ataccatgta
    19441 taaattcttc aaattcatac ccagttacag gtattctcat tgtttccaat aatttcaatg
    19501 ctgcttctaa tgtatctcca tatatatcag agtggcctat tattcttatt tctttagaat
    19561 taactaactt ttttttgttt ctttcaatcc attgtttaga caacttatac actgcttcaa
    19621 atctattaat tgcatctaat atcttattta tttcatcatt gtacttttca ctagatatta
    19681 gtcctttttc tctagctatt tgaagaccta acaacattaa gtttaacttt gtacaataat
    19741 atccttttgt tttagctcca gatttttctt ctccacaatt tactgttaat atataatcag
    19801 aaatctcatc tattagagca tttttacaac ccgccataga tgctatttta caccccttgt
    19861 cttcagccaa cttcatagca ttgtatgtag aataactact cccaccttga gatacaccta
    19921 ctactagggt attttcatta tcaaacttga atgtatcttc agttatcata aatggataca
    19981 ttttcacaac atctatatct aataagtttt gtaaatatgg ttgcacttgc actcctgagt
    20041 gatagctagt acctgaacca gttattataa tcttttttag attttctttc ttaacttcat
    20101 tgaataagct atctgcatta gatataattt ctctcattct aactggtgtt tctaacatat
    20161 aatcttgtat tgtcatttta tacgccttcc ttatctatat attcaataaa ttacaattaa
    20221 cttctataac aaacctatag catgtaaaac tattcctgct actatagttc ctatagttaa
    20281 ttttgttaca cttacctttc tctttaacat gtaatatatt acaaatgcaa aggttaaagg
    20341 taacatatta ggtattattt tatctagtat atcttgtaaa attacttctg caccatttaa
    20401 gttaaatttc agaggagtag ttatactcaa catagttgca accattcctc ctacaacaaa
    20461 taaaccgaca gttgtagcca tagacattat tttatccata agtccttctc tttgtattct
    20521 atcaagagaa tttactccta cttgataacc atacttaagt ccaaatattc taagagcaag
    20581 tgctggtata ttatataata gtaagaataa tagtggtcca aagatattac cttcttttga
    20641 aagtgatact cctactcctg ctgctataat cctaaatgtt ccccagaata gtgaatcacc
    20701 aattcctgca agaggtccca taagagctgt tttaacagca ctaattgaac tttcatcaaa
    20761 ttctggatta ttagcattct cttcttccat agctgctgtt atacctaata tagcaggaac
    20821 taccactgtc gttgtattaa aaatctccaa gtgtctttct attgcttttg cttgattttc
    20881 tttcttattg tataacttct ttattgcagg taacattgaa taacagtaac ctaagttttg
    20941 cattctttcg taattaaatg caccctgaag agcaaatgaa cgccaaaaca tacttcttaa
    21001 ttcttttttt gttaatttat tcaaagtcaa tctctccttc tatcaaagta ttaattgcta
    21061 attgaggatt ttgattattt tctttgttat tgatataaaa atttagtaca aatgctacac
    21121 atactccaaa taaagctata gcagttatat ccaatccacc ataagatgct aataaaaacc
    21181 ctataaagaa aaacacagac attttttttg aaaataacat atccataagc aatgcaaaac
    21241 ctactgctgg taacaatgtt cctgcaacac ccaaaccgtc taatataatt ttaggtattg
    21301 catttatcaa tgctgtaact ttatcagcac caatcataat tgctaaaaaa gttggaactg
    21361 atgtacttaa aaagaataat attactggca accacatcat tatggtaact ctcttaaaat
    21421 cagcctgttt agcatagaaa actgcccttt gagaaaaata cgaatttatt attctaacca
    21481 gtactccaag agattgtgct aagactgcta ctggcactgc tactgctaaa gcaacttctg
    21541 caccattacc tgataatata gcaaaagctg tacctaatac acctcctgtt actacgtctg
    21601 gaggagtagc tgcacctatt cctgtaattc ccatccaaat taattcaagc tgtgcaccaa
    21661 tcataattcc tgattttata tctcctaaaa ctaatcctac taacatacct gtaactatcg
    21721 gcctatcaaa catagattga ccaaatatac gcccatctaa tataccaact cctgctataa
    21781 tagctaacat agtagcttgt actaacatag ttaacctcca ttttaactta gtttaattta
    21841 cattaaatta tatttttgat tttgttttga ttttctaata actcatttac ataaacaatt
    21901 ccattttttg cttcctctaa gcctgattta attattagtt ttgtatcctc ttcaactaat
    21961 aaaagtatag ataaaagcat tggtaaattt actcctgtca ataattcaaa tttgaaccct
    22021 tgtaaaagta agtttgtaca tatgtttgcc acacttccac caaacacatc tgttaataca
    22081 acaaacgctt cactatcttt attatttaat aatatacttc taatttcttt ttcagcatca
    22141 tctgctgatt tatcctttgt catacaaaaa tactcaatat tagttaatga accaattatc
    22201 aatttacttg tatcaacaag ttctctagac aattctccat gtgtcgcaat tagaaatttt
    22261 ttcattctct ttctcctaaa ttattttatt aaatttgata gttgtttctt tttatctcta
    22321 ggtaacattc taaattctat atcaattcct aaatcactta tttctttaaa gttatttata
    22381 tcatcattat caacagcaac taaatctgta agtttcttct ttccttcttc atatcttaac
    22441 cctcctacat ttatttcttt taatactcct cctgagtttt tagctataac taaagcatct
    22501 ttggtatttt ttactaacac taatacattg cttttctttg aagaagcaaa tttttctact
    22561 atttctccag actcctctac cccccttata tataaagtta cattgctagg tttagctaaa
    22621 ttcagtgaca tagctttcat ttggtcattt tttgcttcat catttgcaac tagtattaca
    22681 tttatcccta gtgatgttgc ccatccatat gccacctgac catgtatcaa tctgtcatca
    22741 attctcatta aagataccat tttcctcaaa tcctccatca tacattaaaa tttttattgt
    22801 ttgtcctcta atttatctat aaaagcaaaa agtatgccaa atactggcat actttttcga
    22861 atttcattca cttattttgt aatcttgtat tttactgtgg tagaccatag atgatattat
    22921 aaatgtatcc tatctctgaa attgatattt gcacactata actcttttct agcacactaa
    22981 atgcattttt tattaatttt acttcactat gatggttttt gattaacaaa tccatatcag
    23041 tatactcttc aatttcagct tgtctaacta atctttcaat catgcaactt aagtgaacat
    23101 ataaagcaat ttttttatca tttggaagtt ttttcttttg aatattttct atagcgttga
    23161 ttccctcttc tattctctct attatcttct ttgaatctaa aatagttaga ctgtctatta
    23221 ctctaattaa tgaaaaattc tttacaatag aatcatttat gctctttatt ttttcatttt
    23281 ctactactcc actaaatatt ctaaaaacat catcttctcc ttttcctgat attatatcct
    23341 ctaataatat aaaatccacc ccattcacat ttggattggc agttcctact atagccagta
    23401 catcatacat ttcaaaaact ggttctttat ctcccataga aacaagacta tcataatcat
    23461 aaggtattat tttgacatca actattccaa ttaaactttc tgaaagtagg tgttggattt
    23521 ttatagcagt acctatccct gtaaagcaag atgatattat agcatattct ttattttttt
    23581 gaggataata caattgtata ttaggtttag tctcttcatc cataatttct gcaatctctt
    23641 caatgttctt accttgcatt aataaatttc ctacttctag tgcagaaatg gtggatattt
    23701 ggtctataaa taacattgtg ctatttatct tactctttaa atcttcgtat ataagattaa
    23761 gagagcccat atcaaataac acaattaaac cattctttat cctatctccc tctaggtatc
    23821 tagacatttg ttttgataca tcagcaatgg tagcatctat aggcatatca atagcagtga
    23881 atatattttt acccaacatt cgattacata cattcgccat actactggca gtagcataac
    23941 catgtgctat aataatcgca ttaacctgat taaatttcat tcttataatt gtcgacttaa
    24001 taaatataat cattatgatt gtatctacat tattaagata catatccaat ctattcatta
    24061 ttaatgttga tattttactt attatagagt attctttgta actctctttt tttaaatata
    24121 ttagcaattt attttctaaa gctttatatt catcagacaa ttgatacgat tcatctttca
    24181 aataaagata agacgaaaat gccaatacat tattcccatc atattttata ttgttattaa
    24241 cttctaaatt tctaaatata tcctgcatta ttgatattat aagctccata agtacgttat
    24301 tattttcatc attatttgaa aaaactaaat tatccattaa tctagtcact agattagaac
    24361 tctttttact aaatctttct tttgttattt catttttttg tagtctatca aagtaatcta
    24421 aacattttat aaaactttga tatattatgc ttcttttatt tgttccattt gacatttcaa
    24481 tactaaattg tttaggtgaa aagacaatgc ttttgctttt accactttga aattgaaata
    24541 tgtctacatt ttctatataa agatttctag gaaggtcttg aagagtaacg aaaatatttc
    24601 catttcttct ttgatttttt gcataagctc gtccacatgc atattttatt acatttttac
    24661 attcgcctat attccccttg aattttatct ttaaaatcgt atttaatgta atttgagtta
    24721 tctctatagg caaaccaagt accttacttt cctcataaat aaaatgttct acaatctttc
    24781 ttttttcaat actccctctt tcttctatat taggaatata aatatttatg ggtattcttc
    24841 ttaaaaaagt ttgtaaaaac tcagataggc tctctgttgt agcaaatatt aatcttaccc
    24901 tagcttttct aattactcca ttctctccaa ttctggaaaa cgtacctgta tccataaatg
    24961 taaataactt ttcctgacct tcactattta atctatgtac ttcatctaaa aataaaacac
    25021 cgccatcaga ctgttctatt agacctgctc tagattcatt tgccccagta tatgctcctt
    25081 ttacatgccc aaataataga ctagacaata actccaaatt attataatac tgcgcacagt
    25141 ttaacacatt gtatggagaa tttttttcta atacattact acttacacta tatttatata
    25201 ttatcttggc taaaaaagtt tttcctactc cactttcacc tgtcagcatt atcgtaagcc
    25261 caccatttgg ataaagcaca gcagttttta tttgttcgat tacctctttt aagctattat
    25321 cgtatcctat gatactatta aacacaatct cttgctttaa taatttgcac tcatttttaa
    25381 gttcatctaa actttcataa atactttttt ttgtaggtcc tactacatca tttatcttac
    25441 ttttaggtaa aaaaataaca ggtctactat ttatctttat aagattatca gattttacta
    25501 agtcatttaa taatctactt gattgagttc tacttaaatt aaactttttt actatgtctt
    25561 ctgtcactat gccactttct tttttaagtt catcaaagtt gttaaataat gcacattcat
    25621 ttacaaaact taaaatttca tccctcatgt tcaacctcca atacaaatat caataataat
    25681 ataatattag tatatcattt atatatttat gaaaaagata attatattcc tatcttaatt
    25741 ctcttttgta agattgtaaa atcaaacttt ttataagtat aaaatctaga ttttttaaca
    25801 taatctcaag attcttatct tcctatatat ttgatacaga gtttatataa taatatattt
    25861 attgtattta gtataataaa aagtaaaaca ttataggtat tttaaaacgt attagtgaag
    25921 aaaatatttg taacctttat tatattatga atattatttc agatttaaaa aggctataaa
    25981 taaatttatt tttaaataaa tctacttata gcctcacaat aatacttata aattttattt
    26041 ttccacaata atgtatctca aatatagtta aaaatatatc tatttaatta ttaatattta
    26101 agtaataaag tctcactcaa tcattaaatt cctttttata gcaaattact cttatttcac
    26161 ttatgcttaa tacatttata gatataaata ctaaaaaagc aattcaataa accatatgat
    26221 aaacctaatt tatgtctgtt atttgctcat atctttagac ttatctatac aagcaacaat
    26281 agattttata actgccgctc taaatccttc ttcttctaat accttaacag cttctatagt
    26341 agttcctgct ggagaacaaa ccatatcttt taactcacct ggatgttttc cagtttcaag
    26401 aaccatctta gctgacccca ttactgcttg agatgcaaat ttataagctt gttgtcttgg
    26461 cattccagct aatactgctg catctgccat agcctctata aacataaata catatgcagg
    26521 agaagagcca ctaatacctg taactgtatc cattaaatat tctgatacta tctctgcctc
    26581 accaaaagat tcaaacatag ccttaatcat atttaactct tcatctgtta cattactatt
    26641 tttacaaaga gctgacattc cttccccaac aagggctgga gtattaggca ttactcttac
    26701 tattttttta tctgagccta taaaactttc cagactttct atagttttac ctgctgctat
    26761 agatactatt attttatttg catctattaa ttctttaagt ggagttaaag ccttttcgca
    26821 tatattaggt ttaagtgcta ctattaccac atcagaattt gcaacagcta cctttgagtc
    26881 agttgtagta tttactccaa attcttcttt tactttatct aaggcaactt ggcttaagtc
    26941 tgatacagtt atgtcttttg cttttactaa tcctgactgt actataccac ctatcattgc
    27001 acttcccata ttacctgaac caataaatcc taaagttttc atatcaaatc tccttttttc
    27061 tattagtttt attttaaagt agctaaatta actttatttt aactgcttaa aattatctta
    27121 tttttataaa atataacctt attataatta taacaaatta cttttttgta tgtattactt
    27181 actaatatat gtatgattta ttaaatataa atttaacgat tttttcatta aacttaacga
    27241 gctaacttcc tgtttagaaa gttagctcat aattaatcta aaatttttaa aatataatca
    27301 aaccatttat actttattat atcagttacg attttatata gtaagaagtt ttatttgcaa
    27361 gcaatcctag aatgtttgct cagttcttcc tattatctca tcttgtagtg ttctacttaa
    27421 attattgaaa tggtcactat atcctgcaac tctaactatt aaatctttat aatcctgagg
    27481 atttttttga gcctctaaca gtacattttt atcaaataca ttaaattgta tatgatgacc
    27541 atccatatta aagtatgctc tcactagatt agccatattg tctaaccctt tttctccttg
    27601 aactactgat ggggcaaatc tttgatttaa tagtgttcca ccagttttta tgtggtccat
    27661 cttagcacaa gattttataa ctgctgtagg cccgttagta tctccacctt tttcaggtga
    27721 aattccctct gaaactggtt ttgcacaaag tctaccattt ggacttgctc ccataatttc
    27781 tccaaagtat atatgacaag ttgttggtaa catatctact ctgtattctc caccacaaac
    27841 agttggtcta ccagttacct cattgtaata tgcattgaat atctcttgca ttatctcatc
    27901 agcataatca tcatcatttc catatttagg agtcttattt gaaactaaat taagtatagc
    27961 ttcgtatcct tcaaaattag catcaagtgc tttaagtaat gtgtccatat caaatttttt
    28021 cttatcaaat acattatatt ttattgctgt caaactatct gttatagttc ctataccaac
    28081 accttgtata tatctagtgt tgtatcttgc tccacctctt tggtaatcct tgcctttttc
    28141 tatacaatca tcaactattg tagacataag tggacaaggc atgtgttttg cacatatatt
    28201 ttctatcaca gcatttcctt ctatttttat gtcaatcata taatgtaatt gctttttaaa
    28261 tgcttcaaat agttcctcat aagatttgaa atttctaggg tctccagttt caatacctat
    28321 ttgtttttta ctaattgggt catatccatt atttaatgtt aattcaagta tttttggaat
    28381 gttcatatat ccagttaaaa catatgcttc tttaccaaaa catccagttt caacacaacc
    28441 actagttcca ccaagtcttg catcttctat agtttttcca gcttccataa gctcttgaac
    28501 tattgcttca gtattataaa atgctggttg accccatcct tttcttgata tttcacaagc
    28561 tctctttagg aatttttgag gagtcttttt acttatttga acatttgaac taggttgtat
    28621 caatttcatt tcatccataa catcaagtat tatataacta acttcattaa caccatcttg
    28681 accatctgga tttattccac cagtgtttat atttgcaaaa tctgtatatg tgctactttc
    28741 ttttaatgtt ataccaactt ttggtggtgc tggttgattg ttaaatttaa cccataaaca
    28801 ttcaagtaat tcttgtgctc tatctctatc tagtattcca ttttctacat ctctttcata
    28861 gaaaggattt agatgctggt caagtcttcc tggagtaaaa gcatcccata tattaagttc
    28921 agtagtaacc cctatatgaa caaaccaata catttgtata gcttgatgat aagtttctgg
    28981 tttgtgagct ggtacaacat cacaagtttc agcaattagt aataaatctt ttttcttagc
    29041 ttcatctgtt tcttcttctg ccatttgtct tgctttctca gcatatcttt tacctaatat
    29101 aaccattgca tcacaagcta ttgccatagc ttctaaatca gcttttttat tatatgcatc
    29161 taaatcatta agaaaatcta gctcttttaa tcttgcttct atatctttct ttaaatctaa
    29221 aaatcctttt ttgtatattg tatctccaca aactgtatgt cctggagctc tttgctccat
    29281 aaactctgta aacatacctg cttcatatgc tgctaaccac tcaggtgtca ttgcatttat
    29341 tatcttatct cttgtttgtc tctttttcca aaaaggaatt atttcttctt tatgtatttt
    29401 tctagcttct tcagatacgc taaaatttat tatatctcta ttgtgcatta cttctaaatc
    29461 ttccattgta tgacaacaaa tttctggata tgttggtgct ccattaggag aatcaccttt
    29521 ttcacctact attaattctc catcatttat gctaagagtt ctattctcaa tataatgctt
    29581 aaatgataaa gcacgaagta caggaatctc tacacttccc tcatattttt tataagcttc
    29641 tgtcataagt actgctcttt caatagatat gtgtggttct gcattaatac tttcttctct
    29701 taatttttta gttctctcaa aagttcctct tgccatttta ttaccctcca atctcagtat
    29761 tgaaattact tgcttcaaaa agtcttttta tttcctctaa tttttcttct gatggtcttt
    29821 gtagttcttc tccttcatac tttttataaa tcttatcata cttatgttta ccaatattat
    29881 gatatggaag aagactcact gcttgtatat tcaaaggttt taaaaactct atcatttttt
    29941 taacctctaa attttcatca tctacattta ctcctacaat taatggtatt cttatattta
    30001 tgttagcccc taattcactt aaaaccttaa catttttcag tattaagtca tttgattttc
    30061 ctgtaaattt tatgtgcttc tcttcatcaa taagcttaat atcatataaa aacaaatctg
    30121 catatttagc aactctttca taattttctg actttgcata gccacatgta tcaattgcta
    30181 catggattcc ctttgatttg cacatattta ttactccaca aatgaagtcc atatcttgag
    30241 ccattacttc tccacctgac agagttactc caccacctga ttcttcatag aaaattctat
    30301 ccttgtctat ctctttaacc aagtctctta cagaatactc ttgaccaaca atctcccttg
    30361 cattgtttac acaatagtct aagcaagttt cacaaaattc acacttatct tggtctaggc
    30421 atatttttgt ttcacctttg taaattgcct tatgagggca tatatttata catgcttcac
    30481 atcttgaaca tctttcttca ttatacaaaa cttgttttgt atatgtttga ctctctggat
    30541 tatgacacca aacacattca agtggacacc ctttaaaaaa tactgtgctt cttatccctg
    30601 gtccatcatg tatgctgcat ttttgtaagt ttataactaa tggattcata atttttcacc
    30661 cctaaataat atccattcca ttttttcatt tttatgtaat ttatcttcat agaatatacc
    30721 tatgaaaagc atttctacaa ttacataata ctatacttgt tattattttg tctatatctt
    30781 tttagtaata ttaaaaaatt ttattttatt atgatattta tattgttttt gtaaattaaa
    30841 tttaaatatt tattaaattt atttttataa gaaaatatag caataagcca taaacaatac
    30901 atcattttaa ttaatttaga tgtatatact ataaaaatta aataaaaata gagactacac
    30961 tttaccatgt agtctctatt tttctggtca tatttagaaa tttacattta ttgttactgc
    31021 ttttccacct ctatttactg ttatttttgt actagctcct tttgataagg tatacaactt
    31081 tttatttaaa tcattcatac ctgttatgtc tgtgtctcct actttagtta taatatctcc
    31141 tacctttaca ccagcttttt ctgctgatga accagatatt acttcagcaa cataaacacc
    31201 tttatcagta cttaattttg tacctgttgc tgcttcataa tttgatacat ctgtaccttt
    31261 tatacctaaa gttacttttt catattttcc agtttttatt acttcttcaa ctatactctt
    31321 agctgtattg attggaattg caaatcctaa tccttctgct ttagaagctt ttgctgtatt
    31381 tataccaatt acttgacctt tttgatttaa taatggccct ccactatttc ctgcatttat
    31441 acttgcatca gtttgtaaca aaccagtcat agtagttttt tctgtttgta tagttctatc
    31501 taaaccactt attatacctt gagtaactgt tttttggaaa tccaacccaa gaggattacc
    31561 tattgctatt gaaatatctc caacttttac tttatcacta tcagcaaatt ctgctggtgt
    31621 tagcccagtt ttatcaacct taactatagc taaatcaagt tgttggtcaa accatactac
    31681 tttacctgaa gttgttgaac catcattgaa taatacattt acacttgtag cttgaccatc
    31741 tgatataaca tgagaattag taagtatata tccatttgaa tcaactatga ttccagttcc
    31801 tacaccctgt gtttcagttg gtattgcaaa catattatta gtatcaacac tagttgttgt
    31861 aatacctaca acagatggtg tagctttttc tgcaactgct tgataaatat tttgactctt
    31921 accatcatca tttacaatta ttggtgtaga acttcctgta gatttgccta ctaaattatc
    31981 cttaactaaa attattgtta gaaaactact aagtattgaa cttattatag caactaatat
    32041 tactaaactt atccctttct ttcttcttga catctcatac cactccctat ctaatataat
    32101 ataaattatc aaaatatatt tttcccaatt tttcataaaa aaaatgaaat catctatttt
    32161 gagtaaactc cttttcgatg atttcattat attattttaa tcttaattaa accttaacta
    32221 tttaaaatta tttaagtctt ttttctagct cttccttttc tttttcataa cctggttttc
    32281 ctagtaaagc aaacatgttt ttcttgtaag cttctactcc cggttggtca aatggattaa
    32341 ctcccaataa atatccactt attccacaag ccttttcaaa gaaatacact aagtatccaa
    32401 aattatactc atctaattga ggtatattta ttactaaatt aggtactttt ccatctgtat
    32461 gagccaatag agttccttca aaagctttat gatttacaaa atctacagtc ttccctgcta
    32521 gataatttaa accatctaag tctacttcct ctgaatttat agttatattt ctcttacaat
    32581 tttcaacatt aagtactgtc tcaaataaaa gtctttttcc atcttgtatg tattgaccca
    32641 tagaatgtaa atctgttgag aaatctacag aagcagggaa taaaccttta ttttcttttc
    32701 cttcactttc tccataaagc tgtttccacc actcacttac atagtgtagt tgaggttcat
    32761 agttaaccaa taactctaca tcttttcctt ttctatgtaa aattgttcta gcaacagcat
    32821 atctataact atcattattt tttaggtcag gattttgaaa tgcttctcta gcatcatttg
    32881 cacctttcat catagcatca atatcaagac cagcagcagc aataggaagt aatccaactg
    32941 ctgtcaatac agaaaaacga ccacctacat catctggtat tacaaaagtc tcatatcctt
    33001 cttctgtagc taactgtcta agtgcacctt tacttgaatc tgttgttgca taaattctct
    33061 ttcttgcttc ttccttacca tactttttct ctaagtaatc tttaaatatt ctaaaagcta
    33121 gggctggttc tgtagtagtt cctgatttag atattacatt tacagaaata tccttccctt
    33181 caattatatc taataaatcc ataatatatg ttgagcttat attatgccct acaaaatata
    33241 cttctggagc atttctttgc tcttttttaa gtctattcct aaaagaatga cttaccatat
    33301 ctatagcagc tctcgaacct aaataagagc ctcctatacc tattaccaaa agaaaatctg
    33361 agtctgattt tatcttctca gcagattttt ttattctgtc aaactcttct ttatcataat
    33421 tcttaggaag gtctatccat cctaaaaaag tatttcctaa accagtttta ttatgtaaca
    33481 tgtcgtgtgc aacatctaca tatggttgca taacatctat ttcgttttga caaaaaaact
    33541 ctgtagcttt actataatca aaatttattt ttctcattta aatccctctt ttcagcatat
    33601 tcgttttaaa gagattataa cacaaaatat gggcgttttt tttaaaagtt tatcacaaaa
    33661 tcatacatat ctcatcattt attatcagga aatcgattct tctaaatgct ataaccatgt
    33721 acaatttttt aaattttagt caaataaatt tgcaaaaaaa gaaactctaa acgtatcatt
    33781 taaagtttct ttttatttat attctatttc aatttttata ttgttctaaa ctaactaact
    33841 ttaatcatta cctaatctct tcgctataac ttgttgtgct atattgacag catggtcaga
    33901 tattctctct agattactta ttacatctaa ataaataact ccattttcta tactacatag
    33961 attattgttc aatctataca tatgattagc tctacaagat ttctccatta tatcaacctg
    34021 ctcttcaatt tttataacct tgcaggctaa atctacatta tcagttctca ttgattctaa
    34081 tgcataagta tatgctgaaa caactctatt atacataaca tcaagttcag actgtccttc
    34141 ttctgagaaa gaaatatttg aatctatcgc aacttgtgat aattcagcta tatttttagc
    34201 gtggtcacca attctttcta tatcgttgac tgtattaaac aaggtatcca caacttctct
    34261 tgaatcatca tctaaaggtg cttttgaaag tttaagtaag taattcaaaa tagctttctg
    34321 taaatcattg attaatctct ctcttctata tgtcttgtct attttttcgg ttgaatgttc
    34381 tactaaagct tccatagatg catttaaact ttccttagcc ttttcaccca tacgaagtgc
    34441 ttcttttatc gtatttgcaa gagctataga aggagtttct atcattctat cgtctatata
    34501 tttgatagtc ttagtttcat cttcatctat gtcacctgct ctatcaggaa caagttttat
    34561 agctagtttt atgataaaat ttgtaaatgg taaaagtatc aatacagata ttatattaaa
    34621 taatgtatgt gtattggcta tttgtcttgc aacattgctt gggtcccagc ttgtaaccat
    34681 gcttactatt ggtttattca agaatattaa gaatatcact gttcctaaaa cattgaagat
    34741 tagatgcatc atagctgctc ttcgtgcatt tttgttggca ccaatactcg ataacagtga
    34801 agttacacat gtaccaatat tttgaccata aagaattggt agtgctgctg ataatggcac
    34861 tagaccttct gctgccaatg caaccaacat acccatagat gcacttgaac tttgtattat
    34921 tgctgtaatc ccaaatccca ataataatcc aagtattgga tatctaccaa atgtaacaag
    34981 taagtcagta aatccttgat agtctctcag tggctctact gccatcttca taaaatccat
    35041 acctgtaaat aatataccaa aacctataag tatttcggct atatttttta ttctaggatt
    35101 atttccaaat aaataaagta taatacctac acctaatgct attggagcca taccagttaa
    35161 gtcaaaagat accaattggg ctgtaacagt agtccctata ttagcaccca ttataactcc
    35221 tattgcctgt tttaaggtca ttattcctgc atttacgaaa ccaacaacca ttacagtagt
    35281 agcacttgaa ctttgtatta ctgctgtaac tcctgtacct accaaaactc ccataaatag
    35341 gttactagta agtagtttta ttatcttttt tagctttgtc ccagcagatt tttgaagacc
    35401 ttctcccata aggttcatac catataagaa cagacctaat ccacccatta gacttattat
    35461 aatgttcaaa ttatttcctc cttgtaaaga gattactgta tctaaaattt tataacctat
    35521 tgtattttat caaattatag gagtgataat gttaaattca tgttaaatat taaaaaatca
    35581 ttaagtttat gttaaatttt tgtattattg gtgtattttt gtattatttt ttctgcacat
    35641 tttatgccat caactgctgc tgtgactata ccacctgcat atccagcgcc ttctccacat
    35701 ggatatagat tcctaacact cactgattct aaagtatttt catctcttac tattcttatt
    35761 ggtgatgatg acctagtttc tacgcctgtc aaaacagcat catacattgc aaacccattc
    35821 aattttttat caagagatat aaatgcttct ctcatagtct ctgttacaaa actaggcaaa
    35881 cattcactta aatttgtaag tgtatatcca ggtctatatg atggttcaac atttcctaaa
    35941 tttaaagaag tcttattatt tataaaatct ccaacaagct gaactggtgc attgtaattt
    36001 tttccaccta attcaaaagc caatttttca tacttctgct ggaaatgtac tcctgctagt
    36061 ggattatcac tcttaaaatc ttctggtgtt acatttacta aaaatgcact attagcattt
    36121 attttatctc ttgcatgttc actcataccg tttgtaacta tttcaaaatc atttgatgta
    36181 gatgctatga cgctacctcc tggacacata caaaaagtat atgctgttct tccatttgaa
    36241 acatgctcta taagtctata atccgcagct cctaatctag gatgattgta aaagtctttg
    36301 tactgtgctt tatttattaa atcttgtgga tgttcaactc tagctcctat tgcaaaaggt
    36361 ttttgaacta tttttacgcc tcttgaatat aacatttcat aagtatctct tgcactatgc
    36421 ccaacagcta atataattgt atctgtatct attttttcag tatcatttat tactacagat
    36481 tgtatagaat catttgatac aactatatct gttacttttg aattaaacct aacttgacca
    36541 cctaaactta ttatttcatt tcttatattt ttaactacat tttttaatat atctgttcca
    36601 acatgtggct tatgtgagta taatatttca tctggtgctc caaattgaac taactcttct
    36661 aatacttttc tacatcttat atcttttatt ctagttgtaa gtttaccatc cgaaaaagtt
    36721 cctgctccac cttctccaaa ttgaacattt gaattatttt ttaattttct agtagtccaa
    36781 aaatcactta tatctttggt tctgctgtct acatcaagac ctctttcaag tatgattggt
    36841 tcataaccca tttgggctaa aagcagacct gcaaataaac cagctggtcc actaccaatt
    36901 accaaaggat tattctttaa ttttttatca cccataggta catttatata tctgatttcc
    36961 tttatttttg ttacatcttt tatttttgat ttaagtattt tattttcatc tttaacttct
    37021 acatctacag aataaacaaa ttccattttt cctttttttc tagcatctat agattcttta
    37081 tatatactat atttgattaa gtttttttct tttattctta gcttcttaag tacaatatct
    37141 tttaataaag atatatcttt atcaattcca agttttacat tagatattct taacatattg
    37201 ttttcctctc ttagattgta atataaaaaa gctagggtta ctcctagctt tctacaaata
    37261 taacttctat attctctttt actagatttt ctacccaaat ctcatctagc acaaaattct
    37321 ttattttgtt tacaacaagt gttttatcta aatctttttt caagtttttt actaagacca
    37381 ttgcttcaac cgatttatgc aatctttcaa catacagaga attaacttta atcaatacaa
    37441 cccttaattc acacacatat aacttttcat tacctaaggt gatttcatca tcatattcgt
    37501 atacactaaa ttcatatgta ttttcataag gtgttatatt ttctattcct atataatcca
    37561 tttatttatc cttttttagt tataagacct cttataacct tcatgttatt aaacaccttt
    37621 tctaagaagt tctctgcttc ttcattgtct aaaaggttta tttcaaggtt ttcgttttct
    37681 acaatacctt tagatttaca gtactcttta gcaatattta ttagttcatt ttttttatcc
    37741 atagataaat caaactcttt atagtctatt attatatatt gtttggtatc aggtgataca
    37801 ccattattat atcctatttc tatatgataa ataactgcat atatttcttc tccgttgtac
    37861 acaggtacag ctgggttaga ttttataaag ttcgttgata cagcaattgc gtttcctaaa
    37921 ctaagactca ttattaatac ctctttctaa acattataca tatattatac tatagattct
    37981 ttatctatga cttaaagctt tttataatta attaagatat ttttaaatct ttgtatcttt
    38041 ttcctagcgt actagagctt atatcgtatt tctttgatat ttgaacttga gttataggtt
    38101 gtcctgcttc tttcttaaca taatattcaa cagcagctgc ccaaccattt tcagctcctt
    38161 ttatattctc atgatttttt ttatattcat tccaaaactc tatacatgca tctatatttt
    38221 ctttttcaac attttcttta agagtatcta aaactttaca ttcctcttca gctaattcat
    38281 tttctgaatt aacttttgta tcgtttttat ctctctcttt ctttaagtat tcagctatac
    38341 ttgggtctaa aagctgttgt atatacttgt atagtatatg tgtatgataa attaagaatg
    38401 acttcatatc tttgtataca tcttgatatt gattgaatag attcataata tcctttaaaa
    38461 ttatatcttt aacctcattt gatatactta tagttatatc aatcaatatg ttagttccct
    38521 caatatcaac tattctagct atcatacaac ttccgatttt aaaacttctt agaagtttta
    38581 catcttctgt acaaagttca tttccactta aacagtcctt taatactatt tttccagcag
    38641 aaatttcttt tacttcataa atacttacgt atgattcaaa cagacttttt agtattgata
    38701 cttctgactg acttaagttt gcaccatttt cttcaagaaa gccaactgtc ataacctttt
    38761 tattttccat tatgtggtct tgtattaagt aagtattaaa aaatctttca aatttttcat
    38821 ttacactatc attttgcatt atataaaaca tttctttagc tttttcacat tcttctttaa
    38881 acttatcctg tcttgaataa tcatataact ttgaatataa atctgtatat tgtttttgtg
    38941 aaagttcagt ttttctgcat gctctttctg aaacaatatc tttatttaaa caacatttct
    39001 tgtatttttt gccacttcca caagggcata gctcatttct ccctaacaat atgacttcca
    39061 ctcccttatt attaaattaa agtacaattt gttattatac cattttttta ttaatcaatc
    39121 tatagtataa gtaaatattc tattaaattc acttatattt tggtacaata tattaacttt
    39181 aattaggatt tcgataaaat catcaacaat ccctcttgta gaaatgaatt atcgtaaaac
    39241 taattcctct ataaaatttt attaattgat aatagttttt tactttatct attctttttt
    39301 gttatgtgac tctaactgtc attttacatt caacaatatt gtctttttct tgtttatatt
    39361 cactgagaat attttgagca tcaaaattat ttacttttaa tttgttaatt tctatctttt
    39421 caagtttatt atttagcctt aatttcttca ttacatcttc aaatttcaac acattttcaa
    39481 gttctccatt aacctttatt tctaaattaa cttgtttttt gttatgatta tctaaactac
    39541 tttcttgatt aatatattct atgttcacaa tatttttcaa ttcattttct accaataaca
    39601 ctatatcttt atttgttttt ttatctaaaa atgagttatt aaaattattt tttaattctt
    39661 cattttcatt taattcaaca ctttctatat atttatcatc tatatgctta ttacccttta
    39721 tttgataatt atcttgatta tttattttat ttattatatt ttgcttctca ttcttcaaac
    39781 tagttaagtt ttcttccaag taatttacca ctatatcact tgccaccata ataactaata
    39841 ttaatagtat tttaagtaga tttttttctc tattatttaa tctcaaaaat aatcacccat
    39901 ctaacttttt attctaaatc tataatattc tccatctctt acaatactat ctatgtaata
    39961 atattttatg tactttattt tccttaaatt gtctaaaaca tctaaatcac tacacacacc
    40021 ttctatctct aatttatttt tatctatcca tattctagta atactattta ttccaattgt
    40081 ttcatatatt ttttttattt cattaagttt tattttagaa aatttttctc tagttttatc
    40141 tgttgttgat atagtgtgct ttgatttatc aactaactta ttttgactta tataatcttc
    40201 aagttcattt atagatttca catacataaa tgatttaaat aaaatacatg ttaaaattaa
    40261 gactattatt attttataga ctttattaca tgatttagtt tttttgaatc cattaagaaa
    40321 atttattgtt ttatctactt tcacttaaat aatcattcct atgctactta tataatttat
    40381 actattgtct tctaaatctg tatcaaaatt attttctact tttactttat cttttaatct
    40441 taatggattt acttcaatag atttatctgt atatttaaag tattcttcaa taaaacaatt
    40501 ttctatacca tagtaataaa tatacttgaa gtcccttaat aatcttttta taaactcata
    40561 tgattttctc tcttttgata gcaagtatgt ttcttctatt tttttatctt ttgttatatt
    40621 tataatcaac tgactttttt tacattctat aaatactcct tcttctgaaa agttctctat
    40681 taaatttaag tttataagct tttgtaatat atcaaaattt atatttatag tctttggatt
    40741 catatttaga ttttctgaaa tgttcttaat taattgaata atactctttg gaaaaagaat
    40801 tacttgtata ttcaatttat catttataga atttataact ttatatttta ttgaatagtt
    40861 acttatgttt attggaatgt attgtgttat ttcaaattca atcatgctta atatatctct
    40921 ttttttctta gtatctagaa cttcaacatt tctaatgact atatcttgaa tttgaatgtt
    40981 aaagtataaa tttttatatg atatttttga tttatcaatc tcctctttta caaatctaac
    41041 tacatcttct atatatttaa tcttctcaat cgaattacta tcatttttta tattaaagtt
    41101 aatctctttt ataattgatt tatctataaa aactttttta tgactttttc ttaaaaatac
    41161 aagccttata taatcatcat ttatttctaa atttagaatt tttttaattg tagccatctc
    41221 cttcttaaat aaaaataaat tttaaaactt acaacaatca atataaaata ttattttgga
    41281 taaaattgct tagaatttat attcacgctt atatctgttc cgttatcatt tataacaagt
    41341 tcgaatttac ctgatgtact ctgaggaact ggaacagtat ttaggtatcc cttttccttc
    41401 aatgcttcta cacttagaga gtctattata ttttcatcat cattaattgc catactagct
    41461 gctgttacta tattagcagc attcgtatag tctgcattta atttagcact ttcttgaacc
    41521 ttactatact taactaatgc tatactagat ataactccta aaatagttac tactacaatc
    41581 atttcaacaa gtgtaaatcc ttttcgtttt ttatttatca attaaatatt ctctcctttt
    41641 caaaagtttc atctaataaa atttattttt ctttctaaaa actagtaatt gcatcaaaca
    41701 taggaacaac cattgccaaa acaaaaatgc caataattaa tcccataaag acaattagca
    41761 caggctctat aaatttcatt atttgctcta ttttgttatt taattcattt tcataaaatt
    41821 tatttattac atctaggctt ttatctagtt tgccactttc ttctcctgta tttgtcattg
    41881 aaataaataa tttcggaaaa atatttgcta aattcaagga tttacctatg ctatttcctt
    41941 tttttatata gttattagaa atttttagcc tttcataaat aaattcattg tctataacac
    42001 tagctgacat atctatagct tccacaattt gtattccact actaattaaa atggacaacg
    42061 cccttgaaaa tcttgttgta gctactaatt gattaattct ttttacaaat ggtactttaa
    42121 attttaatga attagttaat ttttttatat tatcatttgt tataaagaaa taaatacttc
    42181 ctattgaaaa caatataaaa ctaaatatca gataagctaa attatttgta accactgtag
    42241 atattcccat taaaactcta gttattaaag gtggttcaat tccattactg gtaaaaacca
    42301 cctgaaaatt aggtattaca aatacgaata taaacaacat tgataagata gataatatta
    42361 taagtattat tggataaatt gaaatagtta ttatttttat ttttaatttg tattctttat
    42421 cataataatt agataaatcg ctcattatta tatctaaatt cccactagtt tctcctgctt
    42481 ttatcatact tataaagaat ttagaaaata tacctgttat ttgaaatgat tctgctattg
    42541 agttaccttt ttgaatatga tttgatacta tattaagcaa attctttact tttttacttg
    42601 actgactctc aatgatttca aatatctttg taatttcaca tccagattcc atcagtatgc
    42661 ctatctcttt gcaaattact ctaatttctt tatcactgat tctatttaat actaaataaa
    42721 acttttctaa attacaaaat atatttttag ataatctcat atactataat accttccaaa
    42781 ttatattaat tacaattaaa taaacttaat attttctaat ttaatacatt taattttatt
    42841 ttacttgaat ataatttata ctcaaacatc ttaattaaaa tagattaaat aaatacaatt
    42901 gagcaaacga aatttaacta ttcaattcag tcaaatcatt tcatttacta ctaaacactc
    42961 ttctaatgtt gttatttttt catttattag ccttttacaa ctatcttcaa atgttatcat
    43021 tccattattt tgtgccacgt ctttaatagt actagaatcc tccatatttc ttatacatgt
    43081 ctttatatca tcattaattt ctaaaatttc ataaattgct gttcttccaa agtatccttt
    43141 atcattacat ttttcacaac ctacagctac tttagtattt acatctccac taaaatcatc
    43201 ttttatcata atctcatgac tacaatgatt acaaactttt ctaaccaact tttgagatat
    43261 aacacctata agagatgcgt ttaaaagata tggtggtatt tgcatttcta gtaatctagc
    43321 tatggaagat actgcatcat tagtatgtaa agtacttata actaaatgac ctgttatagc
    43381 tgctcttact gctattttag ctgtttctgc atctcttatt tctccgacca ttattatatc
    43441 tgggtcttgt cttaatatcg ctctaagacc tacatcaaag ctcaacccta cttttgaatt
    43501 tacttgaatc tgacttatgc catccatctt atactctaca ggattttcta tagtcattat
    43561 attcttattt attcctttta aatcatttaa taaggaatat actgttgtag tctttccact
    43621 tcctgtagga cctgtaacca ataatattcc tgctctttta tttattatac gatttataga
    43681 ttttatagct tcctctgaaa aaccaagttc tagcttgtct tttaaaaaag tatctctatt
    43741 aagtatcctc aaaactattt tttcaccata aacagttggt attgatgaaa ctctaatgtc
    43801 aattaaattt ccacccaact ttatatctac cctaccatcc tgaggaagcc ttttttctgt
    43861 tatatccatt gatgccttta attttacaac cgtagacaat gagggataga catcaactaa
    43921 aaactttgat acttccttta gttctccatc taccctcatt ctcactatta aatgttcttt
    43981 aaatggttct atatgtatat cacttgcatt ttcttttata gctttttcta aaagattttc
    44041 aaataatttt tttgcatagt cctcgtcaag ttcaaagcta ttgtaatatt tttcaatctt
    44101 ttcacttata agttctttct tctctttctt caatataatt tctttacctg tagctaatct
    44161 caagtcttgt agagcataaa tatcctcatc ttctatttct acaactaaat tatcatcttg
    44221 aaattcaact ggaaatacat tatatttttt tgcaagcttt tctggtatta acattggcaa
    44281 actgttttcc tctaatataa tcttaatcac cctttatata ctttaattta ttaggtatat
    44341 tcattttttt catagagata ttttcttcag tttttatgct tattttcatt ggataattgg
    44401 tttgtatgct atctaaatat ttactacctt tatctacata attagatagt ttctccttat
    44461 ctccaatgca ctttatttca tatggctgtg ctacaggaac tccatttata tttatatggc
    44521 ttcctgcaag gacaacttct gaatattgat taattctttg accatttatt tctatgtatt
    44581 ttcctccatt taattttaac tcatttatga cttttacaag aatcttatta tagtctatac
    44641 tattagctat attacctgtt ttttcatcta tagcatctat acttaatatt atcccactac
    44701 ccttcatatc tgaatatgat agactatctc taagcgtttc tatttgtgat attttttcag
    44761 aatttttttg attttttttc attgaatcaa tttctttatc taacttgcct ttctctttgg
    44821 ttaattcttt tatagatttt cttgtcattt ttatttcttt tcttattgac ttatctttgc
    44881 ttgtataaac tgtttcttca ttgctataat ctctaaatat ggttccaaca agtataccta
    44941 aaatagtact tgctagtatt ataaacacat attgtttttt catactgtat tgctccttac
    45001 aaatttattt ttatgtgata attttcttta attaaatatt aaacatttac atacgtttat
    45061 tatatcaaag ttatatatta aaattcacct ttagtatata atatcatata ttgaactcct
    45121 atcactaact taccaatatc aagtatgtat ttttaaatgt tattaaaatt atataaactt
    45181 tcaataaaca ttgttgtaat attataaaaa acataaatta gaaaaggaga taataatcat
    45241 gaaaaaacaa acaataacct taggattaac aagtataata gatttatgag gaattggttt
    45301 acttaggcaa atcatttgca gataaaaata ctgttaaaaa aaatttcaac taactattct
    45361 ggatatgaca tactagaaaa atgaaattca actatttcta ctggttttag ttcaaagtaa
    45421 attcatcata cgcttatatt ttcaatcaaa ttatcttttt ttatataaca atttatgatt
    45481 attaataatt attttctatc aaaaaagtaa aatggcatag ccttaaagct acaccaattt
    45541 actaaaaatt atatcataat taacattaat gttaaacata tgcgtttata agttttcctc
    45601 atattttaaa attcttttaa ccaagttatc tcttaatttt cctacttcat caaatctcat
    45661 attaggacta tagtaacttt ctataatatc atgtttagat ttagctctct taagatttaa
    45721 cactgcccaa caaattaaat catctaaaac tttttcatca aatttaatat cttcttcata
    45781 tttaagtagt ttttctctat ttaaaaactg atttaaatca atcgtatctt cttctttaaa
    45841 attcgtgctt atagttatac ctatatctaa gtcagttaat agtatagttt caattttctc
    45901 tggaataagt ggataatggt aaacttctac atttaaacct ttttcaatag ctttattata
    45961 cactttgttt aatagtgtag ttttcccagt tcctatttct ccagctaaat aataaacctt
    46021 atttacatct tttaaaatgt tttctgtata atctaaacac cctacaggag ttatagctga
    46081 accaaataaa tgcttttctc tttttagatt tcctgtgctt ttaactccat caaataacct
    46141 ttttataaat tcttctgtga ctaaatttac tttaccataa tccatacaat cagaatattt
    46201 ttcttctatg tctctaaaga ttggttctgc cgattttaaa aacttaaatg ctctcttaaa
    46261 tgaattacta atatctttat cgtagactga tatttcttgt ctattctttt ctaattcttc
    46321 tgtattccaa taatcaccta gattaattat ctcgtccaaa gctccaggta gctttgggtc
    46381 catagtatgt ggagcagttg catccataag taccactcct aatttcttta taactaagcc
    46441 atccaacgaa cttgggtcag aaggacaatg atataaatca atatcatatc ctcgatgcaa
    46501 aaactcttta taaatttttt tcataagtga ggattttcct actccaggtc caccctttaa
    46561 acaaaaaact ctttttacat tttcaggaat tatatagtca aaaaaagaat atgagccttg
    46621 tgatgtattt ccacctggga atgccttctt tacctttcct tccatattag cgcctcctca
    46681 taatttacaa tatctcttaa ttaagatatt catattataa gaattttatt acagctttct
    46741 gattttgaca aatttaaaac ttgctatcta atactaataa caggttcatt ttatctaatt
    46801 attgtagatt cttatgctat gcctttttat ctataaatgc aatttaagtg tccatatcta
    46861 agtatttgta ttgtaatata gctctatatt acttaaatcc aattgtttcc attataattt
    46921 tatgttaaaa taatattaca aaatacatct gtttttcttc ataaacgggt gaaattccct
    46981 atcggcggta aaagcccgcg agccttatgg cataatttgg tcaaattcca aagccgacag
    47041 taaaatctgg atggtagaag aaaatagtat atgagtacct ttatgtaatt ttacatgagt
    47101 aatctataca aatccttcaa ctaccgtatt tattcatgaa attagacaca ttcaaggtac
    47161 ctaatataca ggtgcttttt ttgttgttta ttttataatt atatcgtact tataaaatct
    47221 attaagattg gagtgttatc atgaaacaaa aatggatagt attgattatc atctgtattg
    47281 gtgtatttat gtctactctt gatggaagta tactaaatat agcaaatcct acaattgctg
    47341 ctgactttaa aattaacatg tcacaaattc agtgggttgt aacagcttat atgcttgttg
    47401 taacagctac tatgttattt ttcggaaaac ttggcgataa agtaggctca aatcgtctat
    47461 acacattagg attttttata tttacaatag gttcttttct gtgtagtatg tcaaacaatt
    47521 tatcaacatt aatttcttca agaatttttc aggcagttgg agcaagtatt ttaatggcta
    47581 ccggacttgg tatagtttcc aatgcatttc caacaaacga aaaaggaaaa gctatcggaa
    47641 tcactggagc tgtagttggt ataggaaata tgagtggacc tgtaattgga ggtataattt
    47701 tagaacattt tggatggcct tctattttta ttatcaatat accaattggt attatagcag
    47761 tctttcttgg tattaaattt cttcctaagc ctgttttaga tgagcaaagc aaaagctttg
    47821 atattccagg tcttctactt ttcgcatctt gcactacgct tatattactt gcaatgaatg
    47881 agaaaggtaa tacaagattg tatttaggta taatagcact tataatcttc ttgctattgg
    47941 cattgagaga agtaaaattt gaacaaagtt ttattgacct acctctattt aaaaatagaa
    48001 actttactgt aggcaatatc attggagtag cttgttattt tcctcaaatg gcagtatctt
    48061 ttctacttcc attttattta gaacaattaa aaaatttatc tcctatgatg gctggttctg
    48121 taatgactgt acatccactt attatggtac ttatagcacc tattgcaggt tctctttctg
    48181 ataaacatgg tgcaaaaaat attctaacag catctttttc atttatgacc atctcactag
    48241 ttggaatggc acttttaaaa gcagattctc cactgtatct tttaattgta tgtttagtta
    48301 tttttggact tgggctagga gcgtttagtt caccaaacaa tagtagtatt ttagcagatg
    48361 ttcctcctca aaaacaggga tatggaggca gttttcttgc aactgttcgt aatttgtcat
    48421 ttgctcttgg aacagcattt tttagcagtt tttttgcaca aagtcttaca tacaatcaaa
    48481 agttcaaaag tcacacatca gcctatgtta tagctagcaa taaatcttat tggattgcag
    48541 catcagtatg ttttataggg cttatactaa ctgttttctt tatgagaaaa acagataaat
    48601 ctatttctta aataaaattt atttctgatt tcatcagtga ttaaattctt aaatatacta
    48661 atttatatca aactttacag tttttttcta atttataaca ataaattgct aatatttatt
    48721 gtttataaac aacaaggatg ccaaaaaatg tccattaaaa ctattttgag gcatcctata
    48781 ttttattttg atattaaaac agttagaact aatctaatgt tattttatat agattattta
    48841 aacaagtatt acgccaacat ttgatagttt aaatttaatc cactgttatc tcatatccat
    48901 gttgattata ttgatttaca atttcttccc atagttcttc aacaccagtc ttttttagtg
    48961 aagaaacagg aattactttt tcgctacttt ccatttgcaa tttttttctt attatactta
    49021 tatgtttttg atattgacct ctagatattt tatctgcctt agttgcaata actacacagt
    49081 tatatccaaa gtgctttatc cattcataca taagcttatc atctgctgta ggttcatgtc
    49141 taatatctac aagtaggaat atagaacata actcttgtct actttctaga tatctttcca
    49201 ttatacctcc ccacttttct ttttcagact tagctatctt tgcatatcca tagcctggaa
    49261 ggtctacaaa ataaaattca ttatttatta aataaaaatt tatagttcta gtttttcctg
    49321 gtgtttgact agttctagca aaatttcttc tgttaagcaa tgtatttatt attgatgatt
    49381 ttccaacatt tgacctacca gccaatgcta tctctgggat tccttctgct ggatactgtg
    49441 atttattcac agcactcatg gttatctctg aacttctaat tttcattatt ttcaccactc
    49501 tttaataaag cttgttctaa tacttcatcc atatgctcta caagcacaaa ctccattttt
    49561 tcttttacat tttctggtat ttcatctaag tcagcttcac actctttagg aattaatact
    49621 tttgttatac cagctctatg agctgctaat aacttttctt taactcctcc tactgctaat
    49681 actctacctc ttaaagttat ctctcctgtc attgctatat ttccaggcac aggtctttta
    49741 gtaagtgctg agattactgc aagagccata gttataccag ctgatggtcc atcttttgga
    49801 acagcaccct ctggaatgtg tatatgaata tcattcgtct tataaaaatc agggtctata
    49861 tcaaacttgt ccactattga ccttatataa gagatacctg tcttagcaga ttccttcata
    49921 acatctccta gctttccagt taatacaatt tctcccttac ctttaagtac atttacttct
    49981 acttcaagtg taactccacc aactgctgtc catgcaagac catttactaa tcctatttga
    50041 gggttaactt ctgcaagttg atacttaaac atatcttttc caagatatgc ctctaagtca
    50101 gacttactta taacaacttc ttcaagagtt gggtcttcaa cgtatttttt ggcaactttt
    50161 ctacaaatct taccaatagt tctttctagt gttcttacac cagcctccct tgtataatga
    50221 ttaattatac ttcttaaagt ttcatcatct atttttataa agttttcttt taaagcatgt
    50281 tctttaattt gttttggaag taggtatttt tttgctatat ttaatttttc ttcttctata
    50341 tatccagata cttcgattac ttccattcta tctaataatg gtcttggtat attacctagg
    50401 ctatttgctg ttgtaacaaa tagtattttt gataaatcaa aaggtatttc taagtaatgg
    50461 tccacaaaat ccttgttttg ctctgggtct aatacttcta gcattgctga agctgggtca
    50521 cctttataat ctgctgccat cttatctatc tcatcaaata agaacacagg attttttgtt
    50581 tgagcttctt ttaccccatt aattattctt cctggaattg aacctacata agttcttcta
    50641 tgacctctta tttctgcttc atctctaact ccacctaaag atattcttac aaatttccta
    50701 tttaatgctc tggcaattga ttttactata gatgtttttc ctgttcctgg aggaccaact
    50761 aaacatatta taggaccttt aagtgactta gcaagagttc ttatagctag atactctagt
    50821 attctctcct taactttttc taatccataa tgatcttcat ctaatatatc ttttgcctta
    50881 gttatatcta atttatcttt tgtctcttta ttccatggta atgagaatat agtatctaaa
    50941 tagtttctgc ttacagatac atctggagac attgaagata tttttgaaaa tttatctatt
    51001 tctttttcta ttttttcttt agttgtctta ggtgctttta tttttttaag cttttctcta
    51061 tactcatctg cttcagagtt tatatcttct tcttcaccta attctttctg tatagctttt
    51121 aattgttctc taagataata ttctttttga actttattca tctgtttttt tactcttaaa
    51181 gttattttct tttctatttt taatatatct atttcctcta ataatataga ataaataagt
    51241 tcaagtcttt ttctaatatc aaattcttct aatatttctt gtttttggct tgattttaga
    51301 tatatgtttg cagctatcgt atctataaat ctatctacat cttctatatc agctaaagat
    51361 attaatattt ctggtgatac cctattccca atatttatat actcttcaaa ggcatcaaat
    51421 acatttctta caaatgcttc aatttcaact tctgtctcac tatccaaatt atcactatca
    51481 aacactattt cttctattac tgctctgaaa tatccatctt cttgttctat tttctttacc
    51541 ttacctcttg atacaccttc aactaaaaca cgaactgtat ctcctggtaa ttttatcatt
    51601 tgcttaactt tacagattgt tcccacatga taaaaatcct cttctcctgg ctcatctacc
    51661 tctgcttctt tctgagaagt taaaaatata agttcttcat ccatcatagc ttggtctaaa
    51721 gctttaaggg atatctctct acctatatca aaatttaata tcatatatgg aaatatagct
    51781 aatcctctta gaggaattaa tggtaattca tgatctattt tagtataatt ttgttccatc
    51841 aacattactc cttttctagt ctaattgctt tgcttttaat attatatatt tgaacaagtt
    51901 aattttcaac attaatgata aattcttaga atactatcat actatattat ctttttatta
    51961 tgatataata ttaatattag tatacttaga tgttatttat cttttggagg tttctattat
    52021 gttgaaattg tttttaacct ttttaaaaat tggtgcgttt acatttggtg gtggatacgc
    52081 tatggttcct cttatacaag atgaacttgt aaacaaacaa aaacttttag agcaggaaga
    52141 atttattgat tatttatcca tagctcaaag ttatcctgga gtactggcag ttaatatatc
    52201 tgtattatta ggatataaac tttatggtat accaggagtt cttatatgta ccttaggctc
    52261 tgctttacca tcattttgta tagtcttagt cttatcgtat ctatacttta aaaattcatc
    52321 atctaaaatt ttagatggat tttttaaagg agttgcgcct gttgtaatag ctcttatatt
    52381 gtattctttt acgagcatgt tcaagaaact accaaagtct aaaactaaca tactactatt
    52441 aattatagca gttttatctg ttggtgtatt caatataagc cctatttatt taataattgg
    52501 aggaggtgta tatagtctat gtcagaaata atactactct tcttaatatt ccttaaaata
    52561 ggggcattta gttttggtgg cggttatgcc atgctaccat ttatccagca agaatttata
    52621 tctaaatatc actttataac taatcaagag tttttggatt tacttgctat atcacaatct
    52681 acaccaggtc ctgtagcaat aaatagtgcc acatttgtag gatttaagac tgctggcttc
    52741 tttggttctc tagctgcaac tgttggtgtt acaatttttg cattaatagg tctaactatt
    52801 atatcaaagt tattttctaa gtttaaaaac aataaaaaag tagaaaactt attgacagtt
    52861 cttagaccaa ttactctagg attcatatta tcagcagctt tttcatctgc caaagaagtt
    52921 aactgggatt tatatggaat aattgcattt atagcatcat tttatctttt atataaagga
    52981 aaaattggtt ccattgcaat tgtattcgtc tttggtattc taggtatatt actcacattt
    53041 ttataataat gatacctatt atatttagca tattagcata aaaaaaggag cttttaaagc
    53101 tccttttcat catcttatgc actttcctct tgatccttaa gtactatgat aggatttact
    53161 gagtcttcgt tcacagattt ctctgttact ataaccttct ttatattatc tctagatggt
    53221 acttcaaaca tagtttccat cattacactt tcaactatac ttctaagtcc tctagctcct
    53281 gtatttcttt ctatggcttt tttagcgatt gcacgaagag caccttcttc aaattctagc
    53341 tcaacatcat ctaactccaa taacttttta tactgtttta ctagagcatt tttaggttcc
    53401 tgtaatatct gcattaatgc atcttcatcc aacaactcta aagtagctaa aactggtatt
    53461 cttcctataa actctggtat tataccatat tttaataaat cctctggaag tactttttca
    53521 tacaacttcc ctaaatctaa ttctttttta ctttctattt tagctccaaa acctagtgtt
    53581 ttatctccac cacgtttttg aattatcttc tctaatccat caaaagctcc acctaatata
    53641 aataatacat tagtagtatc tatctttaaa aattcttgat gaggatgttt tcttcctcct
    53701 tgtggtggaa catttgcaac agtaccttcc aatattttta aaagtgcctg ttgaacacct
    53761 tcaccactaa catctctagt tatagatggg ttttcagatt ttcttgcaat cttatctatc
    53821 tcatctatat atattatacc tctttcagct ttttctatat caaaatcagc agcttgaata
    53881 agttttaaaa gtatattttc aacgtcttct ccaacatatc cagcttcagt caatgatgta
    53941 gcatctgcca ttgcaaaagg tacatttaga gttcttgcta aagtttgagc aagcaatgtc
    54001 ttcccagaac ctgtaggtcc taataaaagt atattactct tttgaatttc tatatctttg
    54061 ctactagatt ttttactata tattctcttg taatgattat aaactgcaac tgataaagct
    54121 tttttagctt tttcttgacc tattacataa tcatttagaa tctccatcat ttcctttggt
    54181 tttggtaaag atgtagtatc ctcatctata gtatcttcta tctcttcttg aataatttcg
    54241 tcacaaagtt caacacactc gtcacaaata tatacatttg gacctgcaat taatcttctt
    54301 acttgatctt gatttttacc gcagaatgaa cactttaatt gtcgtttttc ttcatattta
    54361 gacatattaa cacctctctc tatctacggt ctctttgtaa atacttcatc tattaaacca
    54421 tattctttgg cttctagtgc actcatgaag ttatcacgtt cagtatccat tttaattttt
    54481 tctaatggct gaccagttct ttcagataat atctcattta aagtttcttt tatttttaaa
    54541 attctctttg catgaatttc tatatctgtt gcttgacctt gagctccacc taatggttga
    54601 tgtatcatta tttcactatt tggtagtgct agtctttttc cttttgctcc tgctgccaac
    54661 aagaatgctc ccatagaagc agccatacct atacatatag tacttacatc tggtttgatg
    54721 tattgcatag tatcatatat agccattcca gaagttatac ttcctcctgg agaatttata
    54781 tataaatgta tatctttgtc tgggtcttca gcttctaaaa ataatagctg tgctactata
    54841 agccctgctg tggcatcgtt aacttgatct cctaagaaga ttattctgtc ttttaatagt
    54901 ctagaaaaaa tatcataaga tctttctcct cttcctgttt gttcaacaac tacaggtact
    54961 aatgccatgg tttcataccc cctttattct atataattta cagtagcttt ttttaagcta
    55021 ctgtaaatta ttatacgcat ttaactaaat tatatttgca catcattata tactaaacat
    55081 ttcataacgt caactatatt agttagcgtt tattttatta agctattgtt gcattatcaa
    55141 ctagtaaatc tattgttttt cttattttta tttgaccttt tatatcttct ttgtcagcat
    55201 ctcttaaaga agcttctatt ttttctactt ccatattgta agcagaagcc attttttcta
    55261 actcagcctt aaattcttct tctgttgctt caattccttc agctttagtt attgcttcta
    55321 atactaaaga agatttaact aattttttag catcttcttt tctctcttct cttaattctt
    55381 ccatagttct accagtcatc tctaataatt gttgtaatcc aaatccttga tattgtaatt
    55441 ggtagttaag ttcatttaac atattatcta tttgatgttg tatcataact tctggtattt
    55501 ctatttctgt attttcagca actttttcta ctactgaatt tctagtttct gcatctgctc
    55561 tatttttagc ttcttcttct aacttagctc ttacatctgc ttttaattca tctaaagaat
    55621 caaattcact agtatcttta gcaaattcat catcaagtgc agataattct tttactttta
    55681 catcgtttat ttttacatta aatacaactg gttttccagc taaatcttgt gagtgatatt
    55741 cttctgggaa agtaacatta acttctactt cttcaccagc ttttttacct actaattgct
    55801 cttcaaatcc tggtataaat gtgtttgaac ctataactaa gttataattt tcacctttac
    55861 caccatcaaa agctacacca ttttcaaaac cttcgaagtc tattatagct gtatctccat
    55921 cttctaaagc tttatcttct acacttacta atctagcatt tttttcaacc atttcttgta
    55981 gtttagcatc aacattttcg tcacttacag tattctcaac cttagctatt tctataccct
    56041 tgtagtttcc taattcaaac tctggtttaa cttctacatt aagaaccata accaacccat
    56101 tgtctttact tatttcctct atatctaaat ctggattgtc tattgggtct atgtctaatt
    56161 ccttgatagc ttctggataa acttctggga ataatatttc tatagcatca ttatagaata
    56221 taccttttcc atattgagtt tctataacaa ttcttggagc tttacctttt ctaaatccag
    56281 gtatattgta tttatttctg cttttattat aagccttatt tactgctgct tcaaatttat
    56341 tgttatctac tgttattttt aaagtaactt tattaccctc ttttttaatt agttctgctt
    56401 tcatccttct aatcctccta gtgatattta tgtaataccc gataacggta ttataattca
    56461 ttctatttat aattcaacca cttaattata tcacataagt ttttttattt acaagtgttc
    56521 cattaatatc gttgtactta tttgataaaa tttaattttt tgagcgtttt ttgccatttt
    56581 tttagataaa tacaccttta tatgcgtatt tcttctatat ttatgttatc ttcttctata
    56641 tccataatga caaaactttt atacgatact cctcttggaa gtgaagtact tccaggattt
    56701 atgtaaagta caccatcttt aatttctcta aacggagtat gggtatgacc aaatataact
    56761 atatcagcat caatttcagt tgcctttttt tctaacatat gcgttccata ttttacacca
    56821 tacttatctc catgacataa gaatatagtt ttatttgcca cttcaaagac aacttcttcc
    56881 tcaacagcat caaagtcaca attaccttta actgcaataa tacctacatt agtcatgcta
    56941 tgaatatatc ttgaatctgt aaagttatct cctgcatgaa ttattaggtc acattctttt
    57001 aaatatggta ttgttttttc catattttta atcatcatat gtgtatcgct tactatgcct
    57061 attttcatta cttcactacc ctttttataa actcttgttt cataagtttt agtgcatttg
    57121 ctctatgact gattgaattt tttataacac taggtatttc accaaaagtc ttatcatatc
    57181 cttttactat aaataaacta tcatatccaa aaccattttt acctttttct tcaaatccta
    57241 tcattccttc acaaattcct cttactacaa attcttttcc atcaggaaaa actacggcta
    57301 ttgcagagac gaatcttgca tttcttttac tcattggtac attttgcata gcttttaaca
    57361 gtttttttct attttcttca tccgttgcat gttctcctgc atatctagct gaataaacac
    57421 caggtttttt gcctaaagca tctacttcta agccagaatc atctgatatt gctataagtt
    57481 tagtcttttt tgctattgtt ctagctttta tcaacgcatt atgctcaaat gtttttccat
    57541 cttctactat atctatacct gctaaatcaa catcttttaa agagtatact ttatactcaa
    57601 aatcttttaa gatttcacct atttcttcta gcttatgagc attattagta gcaatcacta
    57661 cttcattacc ataatccata cccaatattt catctgctat ttcacctaaa gcttcttttt
    57721 gaagccttat taattcttta ttcccttttt cacctaactc taacaataaa tttaaatctg
    57781 ttctcgaaaa aggtttctct tctccagtcc cctgtacttc tacaaactca cacttatcag
    57841 tcattattat attcatatct acctgtgctt tagagtcttc ttcatagcat aaatctagca
    57901 tgtgtgttcc atttacaata ccaacactaa ttgcagatac aaaatttgtt attggcattt
    57961 cttttactgt ctttgcttta tataatttat atattgcttc tgcaactgct acaaatgccc
    58021 ctgttataga tgcagttcta gttccaccat ctgcttgaat tacatcacaa tctacccaaa
    58081 tagttctttc ccctaattta ttcaagtcta caacagacct tattgctctt cctattaatc
    58141 tttgtatttc ttgtgttctt ccatcaattt ttcccctaga tgcatctctt atctttcttt
    58201 gttgtgttgc tcttggtagc atagaatatt cagcatttat ccatccagtg ccactatttc
    58261 ttaaaaaagg aggaactttt tcttccactg atgcagtgca tataaccttt gtctctccca
    58321 tttctataag aacagaacct tctgcatact ttgtataatc tctagttatc tttacaggtc
    58381 ttatctgctc gttacttcta tcataacttc tggtcatttt ttatatcctt tcttatattt
    58441 ttgtttttta taaatttatt tttataattt ttaattttgt caaatattaa ttattgtcat
    58501 ttttcgtaat cttattagct tactgaacta ttctatcatt aattatagta gttttctact
    58561 aaattaagcg actttttaac tctctaatat agcttttact tcatctttcg atgataactt
    58621 taatagttct aaaaatctct cttcatctat aactttaatt cctaaatcat ttgctttagc
    58681 gagtttagaa ccagcttctt cacctgccaa aacaaatgtt gtcgatttac taacactaga
    58741 tgtagctttt ccacctcttt tttctatcat atcttttgca tcatttcttt ttaatgtagg
    58801 aagtgtacca gtcaatacga ttttcatccc ttcaaatatt ttttctactt tttcacctgt
    58861 attatccaat gattttgtat ttactcctac attttttaac ttgtttataa cgttcatatt
    58921 tttgtcttcc ttaaaaaact cgacaacgct gtttgccatt atacttccaa attcctctaa
    58981 ttcttctagt tgttcactcg ttgcagacat cacatcatct aaatccttaa agttatatgc
    59041 aagtatctta gctgctttaa ctcctataaa tttaatacct agtccattta taagtctcca
    59101 taaatcattt tcttttgatt tttcaattga atttattaaa ttgtcagcag atttttcgcc
    59161 cattctctct agctcgacta catcttcctt ttttatataa tataaatcag atacatcttg
    59221 aataattttt tcatttaata gcagtgtaat tatagattct cccaatccat ctatattcat
    59281 tgcatccctt gatacaaagt gtataattcc ccttcttatt tgagctggac aagacatgtt
    59341 tatacatttt acagcagctt ctccttctaa tcttattgtt ggctctgaac aaactggaca
    59401 cttttctggc attttaaaat ctatttcatc tccagtacgt tcttctttta taacttctaa
    59461 aacttgaggt attatatccc ctgctttttg aactaatact gtatcattta tttttatatc
    59521 cttttctctt atataatctt cattatgaag tgtagccctg cttacagtag tacctgcaag
    59581 tctgactggt tcaagaattg ctgaaggtgt tattgtacca gttctcccaa cttctactat
    59641 tatatctact attttagttt tcttcttttc tgctgggaat ttatatgcaa ttgcccatct
    59701 aggacttttg gctgtatatc ccatttcgtc tctttgtttt atattgttta cttttattac
    59761 cataccatct atttcgtatt ctaaattacc cctgttatct gtccaatatt caatgtgttc
    59821 aataacttca tctatacttt tacaaacttt ataactctga cttactgaaa atcctagttt
    59881 ttctaagtac tctagcgagt cactgtgact ttctaaatcg taatcttcta tattttccat
    59941 attaaataca aatatatcta atggtctctt tgcagttagt tttgggtcta attgtctcaa
    60001 tgaaccagct gcaagattcc ttggattggc aaataatggc tgttcttgtt cttcttgttg
    60061 ttcatttacc tttctaaaat tatcttttga tatatatact tctcctctaa ctattaactc
    60121 ttttttatca tttatcttaa gaggtatgct ttttactgtc attaggtttt gagaaatatc
    60181 ttctcctaca actccatctc ctctagttgc tcctttttca aactctccat tattataagt
    60241 tagacctaca gaaagaccat ctattttaaa ttcaacaaca tattcaacat tttctccaac
    60301 atattcttga accctcttat caaaatccct caaatcttgc tctgaaaaag catttgataa
    60361 actcaacata ggagttttat gtacaatttg attgaattta tctaatggct tacctcctac
    60421 cctatttgtt ggcgaatcaa ttctcttcaa ttctggatgt tcttcttcta aagaaattaa
    60481 ctccttcatt aacttatcat attcataatc tgatatttct gggctatctt gattataata
    60541 cctttcatta tggtaattta ttgtctctat aagtttatta atttcatctt ttatgctcac
    60601 tttttgcacc tcttcatatt ttttaaatta aattattttg aaatttcctt ataattaatt
    60661 atctcctatt tatatatttt ttagtatatc acttttttga ctttaaattt attacaaaaa
    60721 aaactcttat tagtatatct aatacgacta ataagagttt tttattttta tcaaaatttt
    60781 ttatttttag tatcctcttg atatgtctac aacgttatct acaggtttat tttccagtat
    60841 atagtgtttc aagttattat aaaccatatt aaatgttctc tctttgtttt tatctgacac
    60901 ccaagaatta tgaggagtta ctatgacatt ttcacactcc cataacttat tatccttgtt
    60961 taaaggttca ctttcaaata cgtctaatgc tacccctcta aatttttcta tacattcttc
    61021 taaatctttc tgattaactg tatttcccct acctacattg ataaatacag aaccatcttt
    61081 cataagttta aacatatctt tatttactat tccatctgtg ctttttgtag aaggcattgc
    61141 tacaactact atatcacatt cttcaaatac aatattcata tcctcagtgg caaaacactt
    61201 atcaaagtat tttttactac ttcccgcagt atttacaccc catatttcta gaccaaaagc
    61261 ttttaatctt tttgctgctt ctgaagatat agtacctgtt cctaaaaatc caactctttg
    61321 tccatacaat tccgatacag aaaaatccat cttccattct ttattttgtt gttgtttaaa
    61381 aaattttgca ctatttttat atatttctaa aatatacaat acaatgcttt caccaatagg
    61441 tacactatat ccgcctctat tattagctat cttaatattt ctcttcaata cttcttcttt
    61501 tggaagttgg tctataccta cacttgttgt ctgtatatat tgtaaatttt tcatcttaga
    61561 gatatctaat ctctcaaatg gattgtatgt aactagtaca tctgctgtat ttacatcctc
    61621 attattttca ataatatctt cagaataatg tatgacatca tagcctaaat ctcttatttt
    61681 atcaaatttt tcttttccat aatttattgt aaataaaact ttcatctcaa atctcctcca
    61741 tctactcaca ttaaaaattt tatattctaa agtataatat gatactcaat gcacattagt
    61801 taaaattact agaagatatc ttaaatttac ttatgttcaa ttgtttttcc taaagacata
    61861 gcattacgtt cttgaactaa attttctatt aaattattca atgtccaatc aatagatgtc
    61921 tctgttacca tagctgtaaa aggtctattt atcctcactt tttttagtcc ttctaaaaat
    61981 gcatttacct gtgtacttgg agcgttatta aatattacag acttactctt aatcccattt
    62041 tcacattcac ttgatatatt tccatctaaa atgtctctta ttatattatc tccattttta
    62101 tcacttaaga tgatatggtc tcttattcca gcaaagttac ttacattttt aattaatgat
    62161 aattcttttt tattaaaatt tacaagcata atacacttcc tattgtgtat tttatctagg
    62221 ttattattat ttattttttc aaatgacatt ttatccatcc tttcatttat ttatatattt
    62281 taatttaccc catacattta aaataatcaa tttttatagt taatttttat attaataccc
    62341 aaattatatt taaatagtta gactatccaa aactaatata taaaattaga ataattagat
    62401 taatatctac aaatcataac ctaaaggaga gataccatga atacattact aaacaataaa
    62461 tttagcatta aaaatttttc aaatgcttgg gttatagtgc ttggttgtat gttgatacaa
    62521 gctgttccat ttagtattgt agcaagtata caacctcaat ttataagtta tgttgtagaa
    62581 ggagaaggct ttagtttagc ttctttttct ctaatattca ctattggtac tattgcctca
    62641 gcaattgcat caccttttat aggtgtatta ttttctaaag tcaatattaa gatactatat
    62701 ttattaggtt gtataatttc tagtattgct tttgctactt tttctatatg tactgaacta
    62761 tggcaatttt acacggtatc atgtattgtt caaattggtg ctacaattat atctgctata
    62821 ggaatacctg ttttaataag tgcttggttt gatgaagaaa gcaaaggaaa agctctaggc
    62881 tttgcttttg ctggtggttc tattggtagt atatttctac aaccacttgc agttcgctta
    62941 ctatcaactc aaggatatag tcgttcttat ctaatatttg gaattgtagc attaatagtt
    63001 ggtatacctg taatgtatat caggtgacac cgtatgatat gattggaacg ccattctaat
    63061 gcggtatcta cgggatttgt tgaaaagtca aagtatcgta aaatgatgtc aaatattggg
    63121 ctgtatcgca aaattgggag tacaaaggga gtacagaaaa aatcctatgg gagtacaaaa
    63181 agtatgatat aaactgtctt ttggaacaat attatgaggg cgtattgtgt aagcgatatg
    63241 ctctttaatt tttgagaaaa gtaatgctac agactaggaa atatgataga atgacagaaa
    63301 taaataggaa ttgcagtatt gagaatgagg aggtttagtt tatgcaatat ttattgataa
    63361 ttatcatagg acttttgctt tttattgaag ttgataagtt gaaaaaacag gtaaaatcac
    63421 aacaaaagca gattgatact ttgtgtgaac agacggggaa taatcaactg gcaacgtatc
    63481 atatatcgga tgaggataaa gaacttattc ttcatttgaa aaatagtgga aaagatgtag
    63541 aggctgtgaa aaaagtaaga gagattactg ctatggaatt agaagaagca aaacaatatg
    63601 tagactctct ttaagagttc aattttctga tatagtaaaa agtaagattt ggataatgga
    63661 ggtgttcaaa tggataaaag acattggttc ttacttggtt atttaattat tccagtatgc
    63721 ttttttgttg cagttattgt agttggtttg ctaagaagtc attctttaat tgaaatttat
    63781 aatgacggat taggaattac ggcattatat taccttttac ttagtctgtt tgtatatatt
    63841 cgctggcaat atttttctga caaataaaga atttaggagg ttaagaaatg tatattattt
    63901 atacagtgtt agccgctata gtgggtatgg ctttggtagt taccgttgat atgggaataa
    63961 caaagatatt tgataaaaag agcatttttc aaaaaaatta tattaagatg ccactttctt
    64021 atttcattgg gatttttgtt tttcttttga ttgtgagatt gattttttca taaagaaata
    64081 gagttgtaaa ttcagctttg tagcagacag ttttaatgta actgtctgct tttccttttt
    64141 aatcataatt gataatagtg ctataaaatg gttcatatcc attgtgaaaa gaccatttag
    64201 agtgatatag attgattata agagtagtag gagtcccgtt gatttgatta tgttctgctt
    64261 cgatttccgc taccacttca tcggtattcc agtattgata tacgataact tgcaattcgg
    64321 tatcacgatt agccccattg ctgaatgaca tactgttaaa tacaagataa tcgggcaagc
    64381 taatgtaata gagaatgcct gcaagcaaga ctagaataat agtggcaaat aacaatttct
    64441 ttttcatttt gacatacctc agtttctata tagttatagg gataactata tagtacctcg
    64501 ctatatagtg aattgctaca taaagattta ttgaatatac ttttatgtag aggtgaggca
    64561 atgaagtatg gacatttgga acttcacata gaggaactgc tgaaagaaaa aggaattagt
    64621 aagaatacca tttgtaagga attggatata ccgaggtcta actttaatcg ttattgcaga
    64681 aatgaatttc agagattaga cgcacaacta atctgtaaac tgtgcagtta cttggattgt
    64741 ggaatagaag aattgataga gtatgtgaaa gaatgagagc ctgtcggatt gaagaaaatt
    64801 cgtgcaggct tttgttgtgt caagaaaaag agaaaacgta gttaggtcaa ggaacaaggc
    64861 gaagccgata cgccgtaggg cagtccttga cggaacggac atagccaaca gcaccatgat
    64921 acagtagagg acttgaaagg tcagaaacct tgcaagtcct cttgtctgtt actggtcgtt
    64981 atgttttaag taaagtgctt ctttcatcag ttctttttca aatgccttga tgtgaccgcc
    65041 ctgtattccg ttgttgatat gaaagcaatc gtcagcctgt gagtaggata gcatggtaac
    65101 tttcggctca taggtcttgg tatcaattcc ataatagtaa atcgtggcgg ttgttaatac
    65161 catgtgaatg ctttcaaaat ctcttgtaaa aattgtatgc ctgtcggaat agaagaaatc
    65221 agacggacat tcatagaatt gggaactatc ctctttcaaa aatagtggca gtatgtgcat
    65281 tgcatttcca gcacgattga caaggacata aaagcgtgga atgagggcgg catattgtgt
    65341 catcatggcg atatatatat catctaaatc atcaagaaga tactcagagc aggagttgtt
    65401 ataaaagaca tattgcaggg cgttatcgtt ttgacttgta tgttccatag gcacattttg
    65461 tccgtaaagc caacgcaagt ccacatctaa agagtcggca atcagttcca ttttatcggc
    65521 tttcggattg tactgtcctg aaagatagga actgaacgct cctttgctga taccagtatc
    65581 tttgactata tctacttgtt ttttattttt tgctttcatg gcaaatttaa tccgttctgc
    65641 gattgtattc atctgaaaac cctctctttc catatcccgt tataactttt catatcttat
    65701 cgtctaagga cactatacca caagttcaga aaaaagaaaa taaaaagttt ataaaaaact
    65761 aaactttgtg ttgcaaaaca aatatgtctg tgctaatata caattaagtt tagaaataac
    65821 tgaactaaaa cgacgtcgta ataggaaaat gaaaatatga gaaactgacc tatcggttca
    65881 acggggagaa aggaacgtgt atgcagatta cattgacaaa agaacaggta aaagaagcat
    65941 ttggagaact tcgttttatg ggtgcggata actgttaccg ctatgataaa gacagtaaga
    66001 agaaaacaga ggaagtggaa gcattaaaac ttcatcttgg aagcatgaag cttgggaaca
    66061 gcgtggatat tcgcttggaa gcgacggaac ttccaaagat tgagccttat gctgtggtgg
    66121 aactggaaga atcagtatat gccccttatg tgcagagagg aaacttccct gtattagtgg
    66181 agaagattac ctgtaagggt atccgccctg ttccaaacaa aaatatgtag gcggtcaaag
    66241 ttcctgccct cgttctgacg taccaaacgg caggttgagg gcagggcaat ggcaacgcca
    66301 ttgcagatag gaggtatggg aaatttggaa gaaagaagcg gaagttttct tccagaactc
    66361 ccctatacta acaggggagt aatacgacag aaagaagcat taagtgcctt gatagactgg
    66421 tgtcagataa ccattaagga agttccgtta gaagcagtta tagaagatgt gctgagaata
    66481 cctttggaac ttatgacggt aacaggctat gaaaagggga ttgcaggaca tgaggttgtg
    66541 gcaatcttcg ataatatcaa agtgctaaag ccgacaggaa atgcacagta tcaaggcttt
    66601 cagattttga tgagtgggaa aggttgtcgc aactatgaaa attttttaca actcaatgag
    66661 gaaacatggt ttgatttttt aaacagagtt tgtcagtacc acatcaatgt tcctcggatt
    66721 gatttggcga tcgacgacag aaaaccgtat ttgagtattc ccgatttgat tgtacggaca
    66781 aaagagggat tgctttccac aaaactaagg gagatagatt ttcacgactc gggagagctg
    66841 aaagaggaag tgtttcagag taagggtggt agtctttatc tcggcagttc agccagcaat
    66901 ttgagattgg tattttatga aaaaggatat gaacagaata aaaaatatgg aacagaatta
    66961 gacgaaaatt ggaacagata tgagttgaga tttcggcagg aaatggcagt gagtgtagta
    67021 caggaattat tgagatatag agatgtggca ggactggcaa tggaagtatt gaacagcaaa
    67081 atgcgatttt tagaaaagcc gacagacagt atgacaacac gaaaaagact ttatccaact
    67141 tatcaagcat gggcggaact gatgaaagac ataggcaagg tcaaactcac catgcaacca
    67201 cagaagaaaa gtttacaaaa ggtgtggaag tggctagaaa agtatgttgc cccgtctttg
    67261 aagttgtttg cagaggttgg gaagatagag cagagagatt atatcggtaa tctagtgaaa
    67321 aatggagaaa tgaatatcgc acagaagcag ttgtatgatg attatataaa agcaagaaaa
    67381 ttagacgaaa gggggtaatc gtatagaaaa ggtattattg aatttaaaag aggtaagtga
    67441 gtatcttgga attggagaaa caaaaacaag agaacttttg agggatagaa attgtccgtt
    67501 ttccatgaag attggctatc ggtggtatgc gaataaaaaa atattagata aatgggtaca
    67561 agaacaatca gagaaaaaag ggggaaatta ttctatttga tatgtatgta agtaagtggt
    67621 atagtgtaag agataaaatg ttctataaag atatttatat catacagaaa ggtgtgtgat
    67681 ataaattggg taagagttta aaaggaaaag aattaggtgc aggactgtat caaagaaaag
    67741 acggaaaata ttgtggtcgt ttctatgacc gctttggtat tagacgttat gtatatggaa
    67801 aaaccatttc tgaaacaaaa gagaagttaa atacagcaat gtatgaagat aaaatggaaa
    67861 tgaatgttgc cgataatact gtgacattag atgaatggta tcagaaatgg ttggaaatat
    67921 acaaatacaa agtgattgca cccagtacaa gagtacaata tgagagcatt tataaaaagc
    67981 atatacaaac agatttcgga cacatgaaac tgaaagatat acagccttta caaatcagag
    68041 ggaaattagt aaatcttgat aaaaagggat ttggatatga aactcagaat aaagtacgta
    68101 ttttactgtt agatatgttt aataaagcgg ttgcggacaa cgtggcaatg aaaaatcctg
    68161 taaggaatat taaagttgtt cgagatgaag aaaaagaacc tcgtgtactg tcaacagaag
    68221 aacaatctgc cttttttgac acctgtaaag gaacatttta cgataatctt tttaatgtgg
    68281 caatatcaac aggacttcgc cccggcgaat tatatgcttt agaagctgag gacttggatt
    68341 ttgagaaaaa ggtaatacat attacaaaat cgttatccta tcagaaattc gagggagata
    68401 caggaaagac ttttcacatg ggaaaaacga aaacgaagtc cagttatcgt gatgtgccta
    68461 tgaatagaag atgtgaaatt gcattaaaga aacagttatt gcagaaaaga gttgtttcgg
    68521 gtagaaatgg tgcaaaaaca ttaaaaggat ttgaaaattt gatttttaca acaaaatatg
    68581 atactcctct taatgcacag atttatgcag atgctatcaa ggtaatcgtt gagcaaatca
    68641 atcttatgcg agaggaattg gaacaatttg aaacattttc ggggcattgc tttagacata
    68701 catttgcaac aagatgtttt gaagcaggaa tacagccgaa aacagttcag aaatatctag
    68761 gtcatgctac attacaaatg acaatggatt tatacaccca tgtgctagaa aaccatttat
    68821 cagatgaaat ggataagtta gatagcaggc tggaaatgat tgaactttca agtgatgatt
    68881 tagcagagca acgttttgaa cgggaatata ggaaacagaa cgaagttttg tcctctgata
    68941 agttaatacg cataggataa aagggagtat aataggagta acagaaaatg ctatgggagt
    69001 acaaagtgct tgaaaatggc ttaaataaag gaaaaatcaa gatatgattt gtactcccta
    69061 gttgttttat tatattattc atacttagga tgcctaaaga tgttaatgaa aaagtaaaat
    69121 ctaaaggtaa aaaagagaaa gcatctgata caatttctcc ttccgtatgg ggttatacat
    69181 taaaagaagc taaaaaaatt aaatattttt ggattatgac gattggattt atatttgttg
    69241 gtatatatgt ttcgtcatta tctgtacaat atccaactta tcttagaaat tatctaaatc
    69301 ctattctaat aggaacagta ggctcgacat ttgctttatt tgctctcatt ggtaatttat
    69361 ttggaggatt attgtttgat aaattcggtc aaataaagcc acttattata tcttttgtat
    69421 tggttcttat agcaacttta tcattactat tggtagataa atcttctgct tttgcatttg
    69481 tatttgccat tttgaaaggt ctttctgtat ttgcttatat gattggtcct gcttatttaa
    69541 ctggcacttt ctttggtcaa aaggaatttg gtagcatata tggtatcgta caattaattt
    69601 ttgcagtggg atttgcatta ggttcaacat tatttggatt actagttgat aattttggat
    69661 atagattttc ttggtctatt ataattttat cagttataat tgcctatact ttacttatta
    69721 taacttctat aggaatgaaa aaattgaata aagaaagact agataaactg atgcaataat
    69781 cgatttttag atatataaat attatatgaa aaagatggag cttttaatca taaataaaac
    69841 tccatctttt tatattacaa tatagctaat tttctcttat ttttaacttt actatctata
    69901 tcctcatcct taactatagt aaatgcctta gttggacaaa catttacaca tgcaggtcct
    69961 tctggtgatt ctatacaaaa atcacactta tttgctgtca ttctttctct aaaacaaagc
    70021 tttccttcat ctacatcttt taatgtaact atgtctattg cacctatagg acatgctaac
    70081 atacaagtct tacatcctat acaattttct tcatttattt ttattgtgtt cccaacccta
    70141 actataccac catttggaca tgcctttgca caaggagcat cttcacaatg tctacactgt
    70201 attggagcac ttacttcttt tgtctttatg acagaaagtt ttggattaaa atttatttca
    70261 tctggatttt ggtaaaaaat atcttcatct gaatgtgcaa ctacacatcc tatcatacaa
    70321 gttctacatc ctatgcattt atcagggtct gctataacaa aatagttcat cacttagcca
    70381 cttcctttcc tatacaatat gtagtgtgta atagatgatg agatttttct cctaatggtt
    70441 ctaccaaata ttcatcatat agttttttaa tatcagggtt ctcatgagat tttctaattg
    70501 ctaaatgttc atcatgcaca tacgttgctt ttgttctatt ttcataggcc aattccttat
    70561 attcttctag taaaattttt ggctgacctc caccgcttac acacccaaca ggacaagtca
    70621 taacttcaat aaaatctaca tctaattttc ctttttccaa gtcttcaagc actggtatta
    70681 cattttttaa tccacttact actccaactc ttaagtctaa gtctcctatt ttaatagttg
    70741 actttctaaa accattttct cctcttactt gttttacttc tacatcagga atactttctc
    70801 cagttataag ctcatatcct gttcttatag cagcttccat aactccacca gtaacaccaa
    70861 agatagttcc agctccacta taactaccta aagggctatc aaatttttct tctcttaaat
    70921 ttttgaaatc aattcccatg tcttttatta aatacgctag ttctctagta gttaacacta
    70981 catctacatc tctatatcca ctagaatcca tctcttctct atcacattca tattttttac
    71041 atgtacatgg cattatagaa acactaaata tctttgatgc atctacatta tttatctctg
    71101 caccataagt tttaaatatt gcaccaccca tctgctgagg tgatttacaa gaagataaat
    71161 gattagttaa tttaggataa tttttttcca tgaattttac ccaagctgga caacaagatg
    71221 taaacattgg aagagttcca ccttctgtca cccttttaat taactcacta ccttcttcca
    71281 ttatagttaa atctgctgca aaattagtat cgtaaactct atcaaaattt aaagctctaa
    71341 gtgctgctgc cattttccca gcacctaagt atcctaaata atatccaaaa tcttccccta
    71401 aagcaactct aactgctggt gcacactgaa ccatagtaaa tacatttggg tcacttaaag
    71461 cttttttaac cttatctata tcacatacat tgtatgctgc aaatataggt tcatttatag
    71521 actctggtat ctctctttct tgttttttct cttgcaaaaa ctcaaatcct tcatctataa
    71581 ctgaggcata agatttacaa gtttgaacac attgaccaca cataacacat ctatctaaat
    71641 ttatctcttg tggctttccc tcttcacctt caatagcatt tactggacag acttttgaac
    71701 attctctaca tccagtacat aaatctttat ctatatttat tatcataaac gtcacctacc
    71761 cctgtatcct ttatttattt gttaacaata agctaagtgc agcttgtcta ttcttagctt
    71821 ttttgtcatc tattggagta acaagtttta atgcttcctg aggacatgca cttatacaag
    71881 ctattttttc actgtcttta cataagtcac atttataagc tatcttttta ttttcatcta
    71941 aaattacttg ttctacttct ttaccatctt gatattgtgg aagtaaatct atagctccaa
    72001 aaggacatgc taacagacaa gttttacatc ctatgcactt ttcttcattt acagacataa
    72061 tattattttc tttgattata gctttttgtg gacatgagtt taaacaagga gcatcttcac
    72121 agtgtctaca ctgtattggc atacaaaaat tgtcaccttt tactaaataa agtctaggta
    72181 taacaggtat atcaactgtt cctactgtat aaccaacatt attatgctga ttatgcattg
    72241 taaaacaagc tacttcacaa gctctacaac ctgtacattt gtttgggtct gctattacaa
    72301 aagagcctat tttatttttc atattatccc acctttcaat ttgtatattt ccaaccctct
    72361 agttaccttc atcatatata tttatatatt ttaatatttt ctcaatactc atatttcgtt
    72421 tacatttttt gcacaaattg tatgtgttgt tatacacctt atcagtatca ttaattaata
    72481 ctccgcagat ttcacacttt gaatatttta gctcttcttt tttagtacac atatactcat
    72541 tctcactttt tgataactct ctgtggattt gcataaatat tcatccgtct tcctcttaca
    72601 aatccaataa gggttatatt taatttttta gaaagctcta ttgataaatt agtcggtgct
    72661 gattttgata ctacaatagg aatttgagtc atagctgcct ttaatatcat ttccaatgat
    72721 attcttccac ttacagctac aattttatct tttaatgcta tatcatttaa aacacaaaat
    72781 ccaatagcct tatccattgc attatgcctt gctacatctt cacaagtaac tattctttta
    72841 tcaaaattaa atatggatac actatgtact ccaccggtat ttttaaataa ctctgatgaa
    72901 tttaaatttc tatccattat actgtaaatc gtatcataat ttatttttat attgctctct
    72961 acaggagcac atgttatata gtctagtgaa tttaaaaata taatttgctc attttgattt
    73021 ttttgcttct catgaatagt tacttttgca aaacgttcac tctcatttat ctcaaatttt
    73081 aataagcttt ttttattttc tatatatcct tttgttttca aaaatcctac tataagctcc
    73141 tctaaattag atggtgtaca taaaaatgta tttatatatt tattatttaa aataaaacta
    73201 agtggatatt ctgcaattat ttcttcatta tctattgata cttcatgttc atttatttta
    73261 cttacttcta cattaaaaga atttaaatat ttgtgctcca ctagactata actattttca
    73321 aaaactttat ccttactatt ttgttgtaat ttagtttttg agtatatatt gttcataaaa
    73381 tattagcctt tctgaatttt ataaatcttt aatttaataa atcattagat atttgtagct
    73441 gtcattttat ctttcaaact tttatactct tctcttacac atttctctgc taattcttgg
    73501 tcttcaagtt tttcaagctt aacagcacaa tatttatact ctggtgtttt agatataggg
    73561 tctaaatttg ctattgtaag ttcattacaa gccccaaccc accactgata agtcatatat
    73621 gttgcacctt ccttaactct atcagtaact gttgctcttg ttattacact tcctcttctt
    73681 gaactaattc taactaattc atcatcttca attcctaatt ttttagcatc attagaattt
    73741 atttgaacac gccctggttc atcttctaat gaactaagtg ttctgcaatt accagtcatt
    73801 gttctcactg aatagtgtcc aacttctctt acagtacaca aactaaatgg atactcatca
    73861 tcttcaactt ccataggagg tctccattca gcagcaaaca aattaccttt accattagga
    73921 gtagaaaact tttgaccttc atatagatac atagtacctt tatcttccat gctttcgctc
    73981 ttacaaggcc attgaacaca cccttgagct tctatctttt cataagtagc acctaagaaa
    74041 cttggacata attgtctaag ttcatcccat atttccttag tatttttata attcatagga
    74101 tatcccattg ctgtagaaat ttcgcttata atttgccaat ctggttttac atcaccttgt
    74161 ggctctatag cctttctcat caactggaat cctctatctg cacatgtata tactccctca
    74221 tgttcccccc aagaagtagc tggaagtata acatcagcat gaagtgctgt cttattcata
    74281 aatatatctt gaacaatgac aaactccaat tcatctaaag cttctctaac ttctgatgca
    74341 tctgggtcac tttgcactgg gtcttctcca aatatataat aagctttaag ctttttctct
    74401 tttagaacta aatctggaac ttgagttaaa ctataaccat tattaggaga tagttttact
    74461 ccccaggctt tttcaaactt ttctcttatc ttatcatcag ttacattttg atagccagga
    74521 tatacattag gaagagctcc catatcacaa gctccttgaa cattattttg tccacgtaca
    74581 ggtcctattc caacgctttc tctaccaaaa ttacctgtaa gtagagcaat tgatgcaagt
    74641 cccttaacta catcaacagc ttgaccaaat tgacagacac ccattccata aagtatcata
    74701 gcttttttgc cttttgcata ttccctcata gcttttctta taagctcttc tgggattcct
    74761 gtaatctttt cagcatattt agctgtatat ggtttaacaa tttctttata ctcatcaaat
    74821 ccttgtgtat gattttgaac aaattcttta ttgtaaagac cttcttctat taagacattt
    74881 ccaaaagcat ttactaaaac catgtttgtt ccacctttta taggtagatg tatatcagca
    74941 attcttgcag attcagttac tctagggtca gtaacaatta atttagcacc atttttttta
    75001 gcttttacta ttctatttgc cactattgga tgtgaatctg ctccattata tccaaatata
    75061 aataaaacgt ctgcattttc tatctctggt atagaattag acattgcacc actacctaat
    75121 gagtaagcca gaccggctac agatggtgca tgtcagacac gagcacaatg gtcaacatta
    75181 ttagttccta ttgtagctct cataaatttt tgcattatat aatttgcttc attaccaggt
    75241 cctcttgcag aacccgttcc cataatagaa tcaggtccat atttttcttt aatttcattt
    75301 aatcttgaag ctgcatagtt tatagcctca tcccattcaa cctcttctag tacaccttgt
    75361 tttcttatca ttggcttttt tagcctaggt gttagaattt tagggtcatt taggaaatcc
    75421 catccataac gaccttttaa acacaaactt tcttgatttg ttctaccatt agctggttca
    75481 gctcttaata tcttattatc ttttactact agatataatt ggcatccagc tccacagtat
    75541 ggacaaactg ttaaaatttt tttctccata caattccccc cttagattta tcccctgata
    75601 agtaaaattt atcttaaaga taatgagtgt ttttataata tgcaaaatca taaatatttt
    75661 gcaatactat ctattaaatt caacaatttt ctcacaattc cttttaaatt tcaaaaaaaa
    75721 tatgtcataa taatctttat atttcaacca tttaattaaa atattctagt tcttatgaca
    75781 tattttttaa tatactcaat atatattatt taaacttatt tagttttcac ttaaaacaat
    75841 atttctgcca attctatttt tcattctata gtgagtaatt acagtaaata ttccacttcc
    75901 aatcagaatc aaatagtaca taaatccacc atataaaaat accgcatatc ccatcaaagg
    75961 ttgagggaat actgatttaa atatagtaaa aaatgctaat tcattagctc ctacatttcc
    76021 aggtgttggt attggagaaa ttgccatata taaaaatgct tgtaatgtaa gtatatagat
    76081 atagctataa ccttgtaaat taaatgcttt atatatccag aatgaaacac taaagtaagc
    76141 acttaattga ataaaagtta ctattatagt tgaaattaat acttttttat tctttgcaaa
    76201 aaaatttatt gctttactat aatcatctat aaaactttct atagattcaa tcttattatc
    76261 taaaaatttt aaaaacttaa tttttgataa atgttttata gtaattctca taaaatattt
    76321 tattttttga ggatttaaaa taactaatat tatcataatt aagacaaatg aattaatggc
    76381 tatccctaga aacactagag gcataatcat tttcatttgt ttttctaaca tagcaaaatt
    76441 cattaataca agtattgtac aataaaaagt aaccactatt tggaaaataa tagacttatt
    76501 ggtaacaact gcacttgcct tactaagtgg cattttacat tttttaagta catatatctg
    76561 tactggttgg cttcctgaag caaaaggagt tattaaatta taataaaatc ccataatggc
    76621 tagcttaaag ccaataaatc taatattaac tttgtgagtg ctttctataa ttatcttcat
    76681 tactactcct tcaagcatta tgtgacacat tattgccaaa gcacctatga ataaaaactt
    76741 cttatctacc ataaccataa catttgatag cattttaata tccaatgttt taaacactag
    76801 gtaaattgtt atacctatta acaaaaataa aaatgtatac tgtagtaaac tttttctaat
    76861 actctttagt ctatccatta gaaataacca tcctttgttc aagatttata ctaaatacta
    76921 tattattagt atatactatg aatcttaata ttttataaca aaatacctta caattttgtc
    76981 atttacaaaa aatattaatc tatatattaa attttaatat atagattaat attttacaaa
    77041 cattttgtag atatatataa tattttcact aaacagcaat tatctttatt acacccattt
    77101 ataccctatc ccccatacag tttctattgt ataaatatca tattgtttta atttgcttcg
    77161 tatatttttt acatgctctg tgatagtact tatatcactt tctccatcaa aaccatatac
    77221 cgactcatat attctttctt ttgaaaatac ttgtccctta ttttttgcta aaaattcgca
    77281 tatcagatat tcacttttag taaaattaat ttttttacca tcaattaaaa cttctttacc
    77341 caaaaagtta aattgaacat ttgagatagt taataaattc tttttttctc tactttctct
    77401 ccttaaatga gcagttattc tagcccttaa ttctcccaca ccaaaaggtt tggttatata
    77461 atcatctgct cctataccaa gaccatacat aatatcactt tccatatttt tagctgtcaa
    77521 aaacagtatt ggacaatcaa ccttatttct tatagctttg cacacttcaa atccatccat
    77581 cttaggcatc attacatcta gtattattag attaaaagaa ttaaattcaa tatccatagc
    77641 attttctata tcactaatca cctttacttc atgtccatct ctttttaata cattttcaat
    77701 caactttaac acatctaact catcatctat taccagtatt ttagacacac taactcctcc
    77761 atttgcatgt ttaataacat tttaatactt atacttaatt acttttttca attttatatg
    77821 aatcattata ctctcatatt atatcttaca ataactgatt gatgttttac aaataaaaca
    77881 ttttagactc aaaactttat atgtaaaata ttctatactt attttatata tgtttattta
    77941 attataaaaa tgtatttgat aatttattat tgtaatattt taaaaatgta ataaaatcaa
    78001 taataagaaa acatcttata aacatatctt taattattta aattggagga aaaaattatg
    78061 gatgaaaaga taagaagcag taactataat gttccatttg actatgctag aaaaaaattt
    78121 aaagaattag accctcatac aattagtaat ttgagtaata tttctttcaa caatgattca
    78181 aacaagttca tactcaattt ctttgataga caatatatta tagactatcc aagtggagaa
    78241 gtttggaatg atgatggtag tatctgtaaa aacttcgaac ttaaaatttt aataattaga
    78301 tacttaacta atgctaaagg tatacctaga actaataaat atgtgacttt taaggaaata
    78361 ccaggaggaa atgtttacta tccaaacttt ttaaacagaa cgctgtctag actttcggaa
    78421 gtttttacat gtcaaataga caaatacaag ttggttatgg aaaatatggg agctgaaaaa
    78481 gtagatatgg gtgacttagc ctataagttt acatatatgg gtaatacatc tatgatattt
    78541 atactttggt ttggagatga tgaattccct ccaaattcaa atatattatt tgattctaat
    78601 ataatttatt acttcaatgc agaagattta gcagttgttc ctgatacagc tatagatgca
    78661 attttaaata aaataaatca attataaata taaataatat tatcacacag gtatgataat
    78721 ggtgcagatt attggactat taaaaaacct agaaaaataa tagttatata gataaaacat
    78781 attaaaaaat agtggcttta aattttttaa agtcactatt tttttaaaat gatgttcctg
    78841 aaaatatatt aagttagtta ttctac
//
LOCUS       DAMJVM010000006        77208 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.006, whole genome shotgun sequence.
ACCESSION   DAMJVM010000006 DAMJVM010000000
VERSION     DAMJVM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 77208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77208
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.006"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            53..262
                     /locus_tag="PC523_000460"
                     /pseudo
     CDS             53..262
                     /locus_tag="PC523_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
     gene            429..1820
                     /locus_tag="PC523_000461"
     CDS             429..1820
                     /locus_tag="PC523_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474026.1"
                     /translation="MEKLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFS
                     PKGRFDSYPISITVGILVACFVMFSERLDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSLILFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIISPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSIFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCDTGVEKGLIGLMGGVIAAKGGTWGLVVGFILYFVLIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            1998..2585
                     /locus_tag="PC523_000462"
     CDS             1998..2585
                     /locus_tag="PC523_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2474027.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNKYVVNYSNSKSVSPYLLLPLSYIEFAQEETHLFKL
                     LFINDLDLMMEEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGMAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            2618..3409
                     /locus_tag="PC523_000463"
     CDS             2618..3409
                     /locus_tag="PC523_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2474028.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYINKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFRKNTQSNENISIIQGNAMDLSI
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFVAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSVFNVTRLHYVASDGL
                     ALYMREAVDSMNDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(3590..6007)
                     /locus_tag="PC523_000464"
     CDS             complement(3590..6007)
                     /locus_tag="PC523_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2474029.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKTKNILYQDKNFSSVGAYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGNLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIIGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESIPDTVDVLV
                     KLSEENTEKDSASIKTQLKKIALSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMRADLFIILILSLVMFLTVRISLFKPTRIISKISAIEAMQYTE
                     TKFRKKVKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYKHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPCVKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     SYRLNLKVGDTVTFNIFSGNDIIKKKMKIMGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEIEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLQNMLYREGLNISIKAICISSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(6020..6685)
                     /locus_tag="PC523_000465"
     CDS             complement(6020..6685)
                     /locus_tag="PC523_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2474030.1"
                     /translation="MSILQVINLKKYYGQNSNITKALDSVTLYIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDMPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            complement(6767..7798)
                     /locus_tag="PC523_000466"
     CDS             complement(6767..7798)
                     /locus_tag="PC523_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2474031.1"
                     /translation="MNFKKFSCSSIYLIIAIGIIFTAILFNISIYIITDDLKLVTLLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQN
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTNSNGKIRVTTECWVMYTKIDII
                     DNGKGISESHQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSEIGKGS
                     TFSIFLPNK"
     gene            complement(7788..8474)
                     /locus_tag="PC523_000467"
     CDS             complement(7788..8474)
                     /locus_tag="PC523_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2474032.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSAMKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINVFKQKVKAFLNHFTTKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKNILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLQYIKTVYGMG
                     YMWLGERNEF"
     gene            complement(8632..9696)
                     /locus_tag="PC523_000468"
     CDS             complement(8632..9696)
                     /locus_tag="PC523_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HDF2474033.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEVNLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            9958..10629
                     /locus_tag="PC523_000469"
     CDS             9958..10629
                     /locus_tag="PC523_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2474034.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQLIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKVCLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDENIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            10631..11539
                     /locus_tag="PC523_000470"
     CDS             10631..11539
                     /locus_tag="PC523_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2474035.1"
                     /translation="MFILAFIICTVVIFIVSTKINQKRYDELSLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKLEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRVNLEPFSDIKLVHNKKWIIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVANQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            11614..12300
                     /locus_tag="PC523_000471"
     CDS             11614..12300
                     /locus_tag="PC523_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2474036.1"
                     /translation="MSILVTNNLVKHYGKDETKVKALNGVSIEIERATFTAIVGTSGS
                     GKSTLLNIIGGLDSPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            12297..14852
                     /locus_tag="PC523_000472"
     CDS             12297..14852
                     /locus_tag="PC523_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2474037.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKENKKSKKYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETAMEGFVNDYLKPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     AYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILISVRVVHQLNKYSSIESMKNFKITNTKK
                     RILESVKHSNMTKVLSHKYMTQKKGLFVGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNIEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKIMKPTGEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFNIENYNSVSIKKTEDSNS
                     VELSEDIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFSLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINAPLYIGMIILNITISIVAVIIPVRQILKSDIISEINQK"
     gene            complement(15057..16256)
                     /locus_tag="PC523_000473"
     CDS             complement(15057..16256)
                     /locus_tag="PC523_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HDF2474038.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            16375..17262
                     /locus_tag="PC523_000474"
     CDS             16375..17262
                     /locus_tag="PC523_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2474039.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENGKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            17452..18009
                     /locus_tag="PC523_000475"
     CDS             17452..18009
                     /locus_tag="PC523_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2474040.1"
                     /translation="MSDSEYPQMKTLETRNCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKKDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(18250..19110)
                     /locus_tag="PC523_000476"
     CDS             complement(18250..19110)
                     /locus_tag="PC523_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2474041.1"
                     /translation="MKKQKSPILLEMLGLFLGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            19609..21006
                     /locus_tag="PC523_000477"
     CDS             19609..21006
                     /locus_tag="PC523_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2474042.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLTLANQNNSSNGTVYFNAIKNNKNDYIYIIYFPLNISKVNMFFDGSK
                     FVGGKSIVFSIIGIIFLIILLSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     TDIYDSLNILNEEIHRSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSSI
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRLLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDYTLLKRAFSNLIVNAFTYGDKNTKVCVHITLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSKTKIEGTGLGLAITKQIIEVHGGNISVE
                     SILGLGSSFYIIFPI"
     gene            21226..23703
                     /locus_tag="PC523_000478"
     CDS             21226..23703
                     /locus_tag="PC523_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2474043.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMSGTATISVQSKNPDILTNLEDIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLIAADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRHTRGVNALLPKETLSKIINKGSGYTRVLIESEEEMTENL
                     VNKLSEELSTEKYTVSNTINETKIISDARQKIMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTHILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPTVISPIGVIISVIVAIIVSLFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSVLLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIEDVRHMDGIKNILPLYVMNNEISGNGVTLSRLEGTDNIELYNSM
                     FAINYTNFEIKKQVTEAFKDKRSVILNEDTLKKVGLSIGDTITLSKDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRDLFGDTYNWSRTVE
                     EFYNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSNK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            23718..24404
                     /locus_tag="PC523_000479"
     CDS             23718..24404
                     /locus_tag="PC523_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2474044.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILYIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLDKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            24723..25043
                     /locus_tag="PC523_000480"
     CDS             24723..25043
                     /locus_tag="PC523_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HDF2474045.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            25181..25825
                     /gene="trmB"
                     /locus_tag="PC523_000481"
     CDS             25181..25825
                     /gene="trmB"
                     /locus_tag="PC523_000481"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDF2474046.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            25954..26700
                     /locus_tag="PC523_000482"
     CDS             25954..26700
                     /locus_tag="PC523_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474047.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEI
                     PKEALSLKDMDSINLANNYLIDDSVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VNIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLDFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILNDKNDLNTSQEVLITVNVK"
     gene            complement(26829..27107)
                     /locus_tag="PC523_000483"
     CDS             complement(26829..27107)
                     /locus_tag="PC523_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474048.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            27382..27828
                     /locus_tag="PC523_000484"
     CDS             27382..27828
                     /locus_tag="PC523_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474049.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILTTVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKGNDTNLKESEV"
     gene            28098..28550
                     /gene="accB"
                     /locus_tag="PC523_000485"
     CDS             28098..28550
                     /gene="accB"
                     /locus_tag="PC523_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDF2474050.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQATSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADNFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            28566..29927
                     /gene="accC"
                     /locus_tag="PC523_000486"
     CDS             28566..29927
                     /gene="accC"
                     /locus_tag="PC523_000486"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDF2474051.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEENNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            29942..30796
                     /locus_tag="PC523_000487"
     CDS             29942..30796
                     /locus_tag="PC523_000487"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDF2474052.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            30817..31761
                     /locus_tag="PC523_000488"
     CDS             30817..31761
                     /locus_tag="PC523_000488"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HDF2474053.1"
                     /translation="MIENNSEKIKVLENDIKQLIIISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSPYEKVMLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSIVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYTKFRNIGKCL"
     gene            31950..32210
                     /locus_tag="PC523_000489"
     CDS             31950..32210
                     /locus_tag="PC523_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HDF2474054.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGIAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            32363..32734
                     /locus_tag="PC523_000490"
     CDS             32363..32734
                     /locus_tag="PC523_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2474055.1"
                     /translation="MKLKLLVEEYAVCRLNNDTKIPTWIDTERFYSITRTDDELSIVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILNCNGYEVE"
     gene            32763..33608
                     /locus_tag="PC523_000491"
     CDS             32763..33608
                     /locus_tag="PC523_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474056.1"
                     /translation="MIYIIEILKISIINIFCFIGIFIVFGLIFSITESLNNKFIFSSF
                     GKIGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTVMIILIFKILLPDSYMKVTQNIDLNLYVSMINNLDISG
                     FIYIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFAISLITYLFGFDNLNISSLIVKYNIFVSLFMAIGLIFSIVTLIISYILSLVSP
                     FKNFS"
     gene            33931..35058
                     /locus_tag="PC523_000492"
     CDS             33931..35058
                     /locus_tag="PC523_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2474057.1"
                     /translation="MNNDKKVIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNAECIKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRENGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            35162..35797
                     /gene="lexA"
                     /locus_tag="PC523_000493"
     CDS             35162..35797
                     /gene="lexA"
                     /locus_tag="PC523_000493"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDF2474058.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLKVKGESMINAGILDEDYVVVDKKNTAFNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(35916..37073)
                     /locus_tag="PC523_000494"
     CDS             complement(35916..37073)
                     /locus_tag="PC523_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2474059.1"
                     /translation="MIERNIKNKKNSKIKALILLFIVSISIYLTGCATSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFINTGNSDAILIQDGKTFTLIDGGDNDDEKLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALASKDLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILS
                     KLDKVDLLKVAHHGSRSSSSQEFLDKVSPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNNNTSKNDSFKNKQNT
                     SNSAEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYVK"
     gene            complement(37402..38034)
                     /locus_tag="PC523_000495"
     CDS             complement(37402..38034)
                     /locus_tag="PC523_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2474060.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNTSVEETFKKSGIPVKVDKSNGQVTFGERTMFDIDSDVLKPEAKEML
                     KMFVPKYMETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            complement(38027..39385)
                     /locus_tag="PC523_000496"
     CDS             complement(38027..39385)
                     /locus_tag="PC523_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474061.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKIRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERIDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHESKISQNQKILLDEKFTLDDSIKNLSNIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFNDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            39555..40154
                     /locus_tag="PC523_000497"
     CDS             39555..40154
                     /locus_tag="PC523_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2474062.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGDAISIKVQL"
     gene            complement(40301..41902)
                     /locus_tag="PC523_000498"
     CDS             complement(40301..41902)
                     /locus_tag="PC523_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HDF2474063.1"
                     /translation="MSNITNSIKDFRMEINQDFIDKAEKLKPILIEKLIKPKQIVNIK
                     MGKNDWEVIDTKSIDEISNISLGKGESICIDFGNHQVGYVNFKIKPIGSPPDAPTHLR
                     IKLGETLCEIGEDSSTYKGSISSSWIQEETMHIDILPDKINMKRRYAFRYLEIKVLDT
                     SPKYKVIVEDVSCNFVSSVDMSEINYLSNIDDDLIKIDKIALKTMQDCMQTVFEDGPK
                     RDRRLWIGDLRLQAITNYETFKNNDLVKRCLYLFAGLTQNRGQVGACLFIEPKLLVDD
                     TALFDYSLFFISCLHDYYEATKDMEILNELWKVAYIQAKLALERVGINGVVYDSDDWW
                     CFLDWKEGLNKQAGAQGVLIYALKQAHKLAIILGDNEKKEELEYEIDRVVQGAKNHLW
                     DEKQGFFVSGSEKQVSWISQIWCVLADVFDKETNTKILDNLLDNPPDMDIVTPYAYHH
                     LVECLILNNRKEKALECIRGYWGEMVKDGADCFWELYNPNDKYVSPYGSRIINSYCHA
                     WSCTPSYFIRKYFGRDSKEVTSKYK"
     gene            complement(41933..43300)
                     /locus_tag="PC523_000499"
     CDS             complement(41933..43300)
                     /locus_tag="PC523_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2474064.1"
                     /translation="MTTLKVVDEKNVPGIWRQRIGYGSSDLACNLIWQMISLYLLYFY
                     TDIMGLSAVAISFMFVVTRVIDGITDLLVGYCIDKTNTRWGKSRPYFLFGAVPFALFA
                     VLAFSVPDISLSGKLIYAYVTYIGLSLTYTIVNIPMASILPSLTDDTNERTALSTSRK
                     FFGFLGSTIVSYSALTLVGKFGGTDEALGFKVVMMLFAFIGCAIFLFTFSNVRERVEI
                     KSENVTLFQTIKSLKENKPWKLFALNILFMWTGFFIQSSALIYYFSYNVGSKSLATTV
                     ATIMSIIPILANFLVPILAKRLGKRNLYITSAAVQFIGLGIILIANLNISLIIAGSIV
                     SAFGYGIKESIYFSMQADPVDYGIWKTGINTTGTLNSVNGFLGKCAQAISGGLGGALL
                     AWGGYIAKAEVQPASALVAIKIMYVYIPMALLICSMITMKFYDLDKQYPKIKADLDAR
                     KISEE"
     gene            43473..44522
                     /locus_tag="PC523_000500"
     CDS             43473..44522
                     /locus_tag="PC523_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2474065.1"
                     /translation="MSLNKLFQNLYIYNESELFYKNYYFAKQNPESLKLFIDNLDEDY
                     IVNNNLIIPYIYNEKIPQLMKDETYFECNSKDNVILIKHNRYTPQFVHEHIFFEMIYV
                     LSGKCYQVINHHNVHMKEGDICIVSPGVKHSIGVFDDSIIINVLIRRSTFEDAFFDFL
                     RNNNLLSSFFLNSIYKDKQNDYILFHTNNDKSIEISILEMLSEYNEQDRYYNNILNHL
                     IMVFFAKLLRDYEDSIEVPNVTKKGTLNHTNILTYIQDNYVDITLEELANKFHFSIPY
                     CSKIIKEIAGCNFTQLLQRIRLERAESLLMNTNIPIADISNNVGYPNVEHFIRLFNKK
                     YNMSPSKYRKNTTKV"
     gene            complement(44642..45115)
                     /locus_tag="PC523_000501"
     CDS             complement(44642..45115)
                     /locus_tag="PC523_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HDF2474066.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(45117..46208)
                     /gene="eutH"
                     /locus_tag="PC523_000502"
     CDS             complement(45117..46208)
                     /gene="eutH"
                     /locus_tag="PC523_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDF2474067.1"
                     /translation="MSINEIIIYVMVIFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEIMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGFAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            complement(46220..46771)
                     /locus_tag="PC523_000503"
     CDS             complement(46220..46771)
                     /locus_tag="PC523_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2474068.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     VVGGETSYVNECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDNIVSIGVVE
                     SGKVCAGIKMLDRTLKSGELVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESEDVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            complement(46764..47036)
                     /locus_tag="PC523_000504"
     CDS             complement(46764..47036)
                     /locus_tag="PC523_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HDF2474069.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKGTYKEGFMVAA
                     DNAGAGNGDLVLISRGSSARESIEKAHVPVDATIVGVVDSFEVFDE"
     gene            complement(47049..47774)
                     /locus_tag="PC523_000505"
     CDS             complement(47049..47774)
                     /locus_tag="PC523_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HDF2474070.1"
                     /translation="MNYDNLVDLIMEEIYKKVNSNEIKISNKPKAVIVFEQDTNKFNL
                     LKDEFEIVEFDKSIKECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMSGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFISFEDEICKFGVEIIKDLNAITKNKLNIKRELESTI
                     GSKKEDTKALDKDVLNLTSVESVKIDNEFSLDLRNRKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKV"
     gene            complement(47816..48445)
                     /locus_tag="PC523_000506"
     CDS             complement(47816..48445)
                     /locus_tag="PC523_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDF2474071.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGK
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGLTLGL
                     KAPIKQSGDLEGTLSIKISTQHGEVELDKGLMVAKRHIHMTPKDAENFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKV"
     gene            complement(48458..49219)
                     /locus_tag="PC523_000507"
     CDS             complement(48458..49219)
                     /locus_tag="PC523_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HDF2474072.1"
                     /translation="MGVLTENEIKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKTYKYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKAQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLEEKLEDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            complement(49346..49633)
                     /gene="eutM"
                     /locus_tag="PC523_000508"
     CDS             complement(49346..49633)
                     /gene="eutM"
                     /locus_tag="PC523_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HDF2474073.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            complement(49719..51188)
                     /locus_tag="PC523_000509"
     CDS             complement(49719..51188)
                     /locus_tag="PC523_000509"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HDF2474074.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQDKLSKMSQEQIDKIVKAIA
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIISEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISD
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESKKLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHANDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSIDKEELINLLVQKLVKELG"
     gene            complement(51191..51895)
                     /locus_tag="PC523_000510"
     CDS             complement(51191..51895)
                     /locus_tag="PC523_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2474075.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRIDKVGAGIVTLT
                     ICGDVGAVKSALEAGEIAASRVGTLRTSHIIPRIHDEVTDALFKTKEPKVCEVSEDKE
                     KNFEVTLETTDNVSKNLSEKEDVILDVTENSGNIEKLYSDIDKINKEIETVDDILTEA
                     NKGKIENTNIDKKVLEQVKIENFDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLK
                     KEELINLVKKLSRRDR"
     gene            complement(51906..52559)
                     /gene="eutL"
                     /locus_tag="PC523_000511"
     CDS             complement(51906..52559)
                     /gene="eutL"
                     /locus_tag="PC523_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDF2474076.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            complement(52579..53460)
                     /gene="eutC"
                     /locus_tag="PC523_000512"
     CDS             complement(52579..53460)
                     /gene="eutC"
                     /locus_tag="PC523_000512"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HDF2474077.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRTGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            complement(53473..54837)
                     /locus_tag="PC523_000513"
     CDS             complement(53473..54837)
                     /locus_tag="PC523_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HDF2474078.1"
                     /translation="MILKTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWILD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIRVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKYG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            complement(54855..56288)
                     /gene="eutA"
                     /locus_tag="PC523_000514"
     CDS             complement(54855..56288)
                     /gene="eutA"
                     /locus_tag="PC523_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDF2474079.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKVDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVIDTGCLDIGGRLIKIDKISKKITYISPKIDKIIKDNNLNLQVGTIATIENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISVDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCKYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYKKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            complement(56472..57881)
                     /locus_tag="PC523_000515"
     CDS             complement(56472..57881)
                     /locus_tag="PC523_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HDF2474080.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     ILSKIDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGNLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFEDIL
                     MDENYDETNEVSIDNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGSYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFL
                     DREHGTIQILINKDNNYRNIAIIDDGVGFDSDNINKNSLGTYIIDSYITQMLKGNIER
                     KSNKNGTEILITIPTQINI"
     gene            complement(57874..58449)
                     /locus_tag="PC523_000516"
     CDS             complement(57874..58449)
                     /locus_tag="PC523_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2474081.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENLAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            complement(58552..59001)
                     /locus_tag="PC523_000517"
     CDS             complement(58552..59001)
                     /locus_tag="PC523_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HDF2474082.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELNYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKSKNIDEV"
     gene            complement(59009..59359)
                     /locus_tag="PC523_000518"
     CDS             complement(59009..59359)
                     /locus_tag="PC523_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2474083.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            complement(59600..60730)
                     /locus_tag="PC523_000519"
     CDS             complement(59600..60730)
                     /locus_tag="PC523_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2474084.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLVNSTYQVFEEIVPDPPIEVVVSGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSDTAKKYAQIAKLVGLGNANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILNNIK"
     gene            complement(60904..61080)
                     /locus_tag="PC523_000520"
     CDS             complement(60904..61080)
                     /locus_tag="PC523_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474085.1"
                     /translation="MEINVIEIFPKDKAKLNEIEMNKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            61408..63006
                     /locus_tag="PC523_000521"
     CDS             61408..63006
                     /locus_tag="PC523_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2474086.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGIKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFNYLVCYRLDRISRNVSDFSTLIEKLNELNISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEVVKLIYNKYLELRSLSKLYKYIYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILEYLKNNGMDIVGTPDKKCGVLTYAKNTTNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRIATGKTALLSGFIKCGKCGSNMRITYKGR
                     KTDKDLKYYYICGTKKTLGADNCNCKNLNGPLVESLIIDKIKNCKEESIISTFNNNNI
                     KTGNIFNVLKSETNLLKNQILDKEKLIKNLVIELAKNTDSIASDYIVSQIENLNSEIN
                     ILKDKLNTLESSNANISSDSINLDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSNLGNLNIIYMGAEMDNSVSLSN"
     gene            63201..64001
                     /locus_tag="PC523_000522"
     CDS             63201..64001
                     /locus_tag="PC523_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2474087.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYASSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGASKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(64259..65082)
                     /locus_tag="PC523_000523"
                     /pseudo
     CDS             complement(64259..65082)
                     /locus_tag="PC523_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
     gene            complement(65151..65657)
                     /locus_tag="PC523_000524"
     CDS             complement(65151..65657)
                     /locus_tag="PC523_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2474088.1"
                     /translation="MELIEWNYAYITDLVEFANDKEIAKNLRDNFPNPYTEQNARDFI
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTSINKNAVLGYWLARNYWGKGITTN
                     AVKEICKIAFEKYNLNKIHANVYSYNRRSSKVLEKCGFKIEEYCHNSIIKNRNMIDCH
                     VYSLIRTQ"
     gene            complement(65671..66597)
                     /locus_tag="PC523_000525"
     CDS             complement(65671..66597)
                     /locus_tag="PC523_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474089.1"
                     /translation="MSDILSRISNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSSIDPEEIHTLESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARAIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNDTVEMVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            complement(66722..67504)
                     /locus_tag="PC523_000526"
     CDS             complement(66722..67504)
                     /locus_tag="PC523_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HDF2474090.1"
                     /translation="MTKTQQEMIQNVLEFSKRDSRIIGLAISGSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVATHEYRSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            complement(67660..68280)
                     /locus_tag="PC523_000527"
     CDS             complement(67660..68280)
                     /locus_tag="PC523_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2474091.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNAKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     KGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLKCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(69038..69574)
                     /locus_tag="PC523_000528"
     CDS             complement(69038..69574)
                     /locus_tag="PC523_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDF2474092.1"
                     /translation="MQGGVDIRQVKSFVFPVISLIFLDQISKVLIGLFLMGFEIDITG
                     KFLRFNPVQNTNLSYGGNFIGILSNLWVLVLFNILVILVIISGYAFYKSKNEQTSYSV
                     KVIMSCGLAGTICSLIDKLFWGGSLDFLQIPSFFIFDLKDCYLTVAEIIFVIIGILHS
                     REISMKEYIYFCYRQFKR"
     gene            complement(69965..70474)
                     /locus_tag="PC523_000529"
     CDS             complement(69965..70474)
                     /locus_tag="PC523_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2474093.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVELEVDNLKVISEKLEIINLQLVQRKNMRRKMFHWLF
                     ILLCSVIVIVFMVLGGLNSPYLNWNYTNPETAVFGVAFHAFECLFVRLTPIVLIGAII
                     GSFLTRKKI"
     gene            complement(70751..70900)
                     /locus_tag="PC523_000530"
     CDS             complement(70751..70900)
                     /locus_tag="PC523_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474094.1"
                     /translation="MNVNDNLKSINRELCVKTYDNFKILHDKEINRLKELKLNGYKLI
                     SSMKI"
     gene            complement(70921..71007)
                     /locus_tag="PC523_000531"
                     /pseudo
     CDS             complement(70921..71007)
                     /locus_tag="PC523_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606463.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            71887..72324
                     /locus_tag="PC523_000532"
     CDS             71887..72324
                     /locus_tag="PC523_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HDF2474095.1"
                     /translation="MDKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKDCSIYVTHF
                     PCLNCTKAIIQSGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            72463..73251
                     /locus_tag="PC523_000533"
     CDS             72463..73251
                     /locus_tag="PC523_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2474096.1"
                     /translation="MKVVIIPGHTLTGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNVTTSTPGGTETIYKTNNEKTY
                     AERVNTRLATVFKNRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGVNKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALKQIDKL"
     gene            73299..73757
                     /locus_tag="PC523_000534"
     CDS             73299..73757
                     /locus_tag="PC523_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474097.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSNGKADLLYSCRLQKILKEIYINQSRASYMSGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(73857..74207)
                     /locus_tag="PC523_000535"
     CDS             complement(73857..74207)
                     /locus_tag="PC523_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474098.1"
                     /translation="MNNTITINISIIYLLLAILACIALVYLIIVLVKLHKILNTVGTM
                     LNENTQNINTTLNNLPSVVENFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(74209..74592)
                     /locus_tag="PC523_000536"
     CDS             complement(74209..74592)
                     /locus_tag="PC523_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2474099.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLENKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            complement(75098..75481)
                     /locus_tag="PC523_000537"
     CDS             complement(75098..75481)
                     /locus_tag="PC523_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474100.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKEYLYV
                     SSMYAIKEAKLESRINSGRVKQYDG"
     gene            75830..76321
                     /locus_tag="PC523_000538"
     CDS             75830..76321
                     /locus_tag="PC523_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDF2474101.1"
                     /translation="MNNQQQEVYKKLDELNIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
     gene            complement(76446..77183)
                     /locus_tag="PC523_000539"
     CDS             complement(76446..77183)
                     /locus_tag="PC523_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2474102.1"
                     /translation="MKQNKYDDDKFFNKYSKMERSINGLAGAGEWHTFRKMLPNFKGK
                     RVLDLGCGFGWHCQHAVENGATSAVGIDISEKMLKEARNKTTFDNIKYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGDFVFSVEHPIFTAHGSQEWHCDK
                     DGNNLHWPVDRYFEEGLRKSNFLGEEVIKYHKTLTTYLNTLIKTGFEVLEIIEPQPEE
                     KLLDAIPGMKDELRRPMMLLVSAKKDK"
BASE COUNT        27016 a        11020 c        10539 g        28633 t
ORIGIN      
        1 gtttgtaatg attctatatt atatggttaa aaaaaattag aaaaaacaaa ataacgtaga
       61 attattaaca tacaataata tgggacatcc gttaccaatt tcatatatta tgcctctaaa
      121 gaagacttta tgtatgagta tgagtggggg gattttttct tcaggtggaa ctgtagaagg
      181 aaattcaaaa ggacagtttg aatcatggaa tagaactatt gaattttata aaaaaccttt
      241 atcaagtgat ttaaataact aaaaatgaag aggtatttct atatatgcac aaaatctatc
      301 aattttataa atttaattta ttgtttaaat aatttgcatg ttatatacta attttaacga
      361 cattgataat ttataaacaa ttataaagtt gtaaacttat aagagttatt gaaaggtgga
      421 gtgaagttat ggaaaaactc gctagaaggg aacaacctca tataaaattt ggaatgttca
      481 agataagaat tccatttata cattacaggt ttgaaaaacc agaagctatt caaggtataa
      541 tatcaagtat gacaagttta ggcacaattg gtcttagtac acaaatttta ggattaccat
      601 atgaagtagc atggagtatg gctataatta attcaatatt atactgcttg catgttttta
      661 tgggagaccc agttgtacct ggctggatta cagcttctat aactcttaca actgcatatt
      721 tgttaaagtt cagtatgggt attgaaagaa tacaagcatt aacagcttta caaatagatt
      781 taggaatcat atttatttta atgggtataa ctggtgtagc tggaaaatta gttagtaaga
      841 taccaaattc tattaaaggt ggaatactaa tgggtgttgg tatatctact ataatatcag
      901 agtttagtcc taaagggaga tttgactctt atcccatatc tataacagta ggaattttgg
      961 ttgcttgttt tgtaatgttt tctgaaagac ttgatacttt aaaagttaag aataaatttt
     1021 tatttcattt aggagaatat ggtgtagtat cagctatttt agtgagttta atagtgggta
     1081 tatttagtaa agaaatagaa attcctagat ttagttttga taatttagtt tatatagtag
     1141 attttaaaaa tttgataaat acagtatctc cttttggaat aggttttcca agcttaattt
     1201 tatttataca agggatacca atggccttca tgatatacat aattgcattt ggtgatttta
     1261 taacaggtga aaatttagta ctttctgaaa gtgaaaatag aaaagatgaa tatatagatt
     1321 tcaattcaaa taggtctaat gttataagtg ggataagaaa tatttttatg gcaatcattt
     1381 ctccatatat accaatgtgc ggtccattag ctgcgacact tacaggaagt gttgctcaaa
     1441 ggtacaaggt tggaaaggaa gcaatgcagt ctatttttag tggtatgggt acattagtat
     1501 gggtttcagc aatttttatc tgtttttatc caattgcgca gattgctaca cctcttatac
     1561 cattagcgtt gtcagtaaca cttttagttc aaggatattt atgtacaaag ctatcaatgg
     1621 agctatgtga tacaggtgta gagaagggat tgattggact tatgggaggt gtaattgctg
     1681 ctaaaggcgg aacatgggga cttgttgtag gttttatatt gtatttcgtt ttaattgatt
     1741 ctaaaaaaag aaaagaaaaa gatattaatt caatagaaga ttgtgtatat gaaatattag
     1801 ataaaaagaa gagtatttaa aggaaaatgt gagttgctgt tatctattaa tagattaatt
     1861 gcaactcatg ttttatttat cagttttagg aattgcaaaa aaatgcaaag atttttcttg
     1921 acataattct ttgatatgtt actatagtta tgtaacgtat gtattttatt aaaaatttaa
     1981 tggagaggtg aacatatatg ccacctaaag taaagataac aaaagaaatg attttgaata
     2041 cagtcttgga tattacaaga gaagtgggat ttgaagctgt aaatgcgagg agtattgcaa
     2101 gtaaattaca gtgttccact cgaccaattt ttacttgtta tgaaaacatg aatgaattaa
     2161 aaaatgaatt tcttgctttt gcatatgaat actataataa gtatgttgtt aattatagta
     2221 attctaaaag tgtaagtcct tatttactgc ttccgctttc atacattgaa tttgctcaag
     2281 aagaaactca tttatttaag ttgttattta taaatgattt agatttgatg atggaagagg
     2341 caaatgattt ttataaagaa attgacaatg aaaaaaaggc aagacttttt tcggaaagca
     2401 ttggaataga gttggaatgt gcaaaagtaa tatttttaga tttatttctt tatactcatg
     2461 gcatggctgt cttaacagca acaaaaaaat taacattaga tagatatagt gctgaaaaaa
     2521 tgttgaaaaa tatattaaca gcttttataa gacaagaaaa accagattgg aatttgtcta
     2581 tttaatgttt attaactaat atagaggagg tgaaattatg gaaacaaaac aatacttaat
     2641 tgacttttat aatacttatg atgaagatag tcgacttgca ttaaaacatg gaatggtgga
     2701 atttctcact actatgcatt acattaataa gtacataaaa tcaggtgatt gtgtgcttga
     2761 aattggcgct gcaactggtc gctattctca cacactggca cgtcagggat atgatgttga
     2821 cgcagtggaa ttggtcgaac ataatataga ggttttccgt aaaaatactc aatcaaatga
     2881 aaatataagc attattcagg gtaatgctat ggatttatct attttcccag ataataaata
     2941 tgatattacg ttactgttag gaccactata tcatctttat aacaaagaag ataagcaaca
     3001 agctttacat gaggcaattc gtgtaacaaa acctggtggt gtggtttttg ttgcatatgt
     3061 tatatctgat ggttgtctca ttgacgaggg gttccataga ggaaatatta atgtatctga
     3121 atacattgaa aaaggtttga ttgacccaca gacatttgct gctaagtctg agccaaagga
     3181 tttgtttgag cttgtacgta aagaaaacat tgatgatttg atgtctgtat ttaatgtaac
     3241 tagactgcat tatgttgcat cagatggttt ggctttatat atgcgtgaag cagtagactc
     3301 tatgaatgat gatgcatttg cgttatattt aaaatatcac cttgctactt gtgaacgtga
     3361 agatttagta ggggttacaa gtcatgcaat tgacatattt agaaaataag taatatgctt
     3421 gtttatgtct ctggtgatgt tgcttggaga tataaggaag tatatctgta taaaataaaa
     3481 acaagtagag ggacagaaaa ttatgtaaag tcaaaatttc ctatcccttt agcttttaaa
     3541 aaattatgtt aaaacttgta taaaaaatgg atatttttta tatacattct tactgagaac
     3601 gtaagcgttc tgtaagtgat tttttttcta tatttcttac aagtaattca gtcactaaaa
     3661 cttgtatacc aattaatacg aatgaaaaaa ttaaaatagt aaatatagaa aatttaaaat
     3721 taataaagtc taatctaatt acatctttta taaaagtgca aagtagattt gaaccaaaat
     3781 aacctagtat acttgaaata catatagctt tgatacttat gtttagacct tctctataaa
     3841 gcatattttg cagttgcttt cttgtcattc caacggcttg aagtaaggca aactcttttt
     3901 ttctaattaa aacgctgttt attagggtat tggtcatatt tagtatacca aagcaagaaa
     3961 ttaaaccaac aaaaatgtat gaagctataa ttatatattg aaatgctcta gtaatcattc
     4021 tataatcatc ttcatatgaa taaagcatta aatttgaatt tttctgaatt atacttaaca
     4081 acttttcttc aatttcttta gaatgctttt tatctattaa aatagaaaaa tctaaatttg
     4141 tattatatgg tgttagagat tctaaagttt tgtctgaagc aaaaaacata cctcctgtat
     4201 tggaattgtc aataattccc attattttca ttttcttttt aataatatca tttccactaa
     4261 aaatattaaa tgtaactgta tctccaactt ttaaatttaa tctataggat tttgttctat
     4321 atttatttat aattaaacta tctgaatcta aatgtgtata gtctattttt ccacttacaa
     4381 tatattcttc aagtgatttt gaaagttcag gcgtaatacc tttaattaga gtaataaatt
     4441 gtttttcatc tctatctttg acgcaaggtt ctataatatt gcttttaatt gatttggatt
     4501 caattaattt ttttactcca tgaatagatt taatttcatt cttaagagaa tctgttagtg
     4561 gattattttg ttggatttct gtaatatgct tatataagtt atcactctta atgctaatta
     4621 tataatcttc gttatagaca aattgttgag atagttgttt tttgaaatct attgaattag
     4681 caagattagc gattgaaata aataaaactc cactaatgct tagtgatatt aatgcaatca
     4741 aatttttctt tctatttgac tcgatattct ttaatgctag attttttata tttatataac
     4801 taaaattttt tacttttttt ctaaatttag tttctgtata ttgcatggct tcaatagcag
     4861 atattttaga aataattcta gttggtttaa ataaagaaat tctaacagtt aaaaacatca
     4921 ccagtgataa aattaaaatt ataaataaat cagccctcat ttgccatgcc aatggttgaa
     4981 ttatgtaagc aattatacaa gaagctataa cccctaatgg aataaaaata attgacaaaa
     5041 tatttccttg tagagaaata atttttttta actgaaaaga agtcatacct aacgatttca
     5101 acttagcata catctgaact gaattaacaa ctgaaatata aaaaatacta tatataacaa
     5161 aaacactaga aaagagaaca actattccaa ctactaaaac tgtaataatc tgctcaccat
     5221 caattaaatt tgattcaata tagttatcat ttaagtatat attatatttc ttaataccta
     5281 aagataaagc aattttcttt aactgtgtct taattgaagc tgaatctttc tctgtatttt
     5341 cttctgatag tttaactaat acatctacag tatcaggtat tgactcgtca aaagtattac
     5401 ttttattttg taaaggtgta gaagctatat tcttaaaata atcatttgaa acgactgtat
     5461 agtactgctt ggcattatct tgataataat tttctagaaa tccaataata ataaaatcac
     5521 attgaacttc tttattcgta atatcatcat aataatttaa tttaatgcta tctccaatat
     5581 ctttatctaa accaaattcc tgtaaatatt tttctgaaat agcaatttca tttttatttg
     5641 taggcaagtt gcctttcaat ggctgaaaat tcataagttt aattgctgta tcatctgcat
     5701 aaacaataga cataatacaa tctgtctttt tattacttcc aattaatttg taagctccta
     5761 cactactaaa gtttttatct tgatatagta tattttttgt tttttcatct acagatttat
     5821 atatagcatg atatggagat gcattaacta agtctacgga agcattatat gaaactaagg
     5881 tcactgttaa taataaacat gtagaaatca ttataatgat acctgaaaga tatgttttaa
     5941 gcttattaat ttttaaatca tttaaagcta tttttcttat aaatgtgcga ttattatttc
     6001 taaacatgta ctacacctcc tagttaacaa tttttccatc ttcaatacga ataattctat
     6061 cagttagttg tgcaatttca ttattgtgtg taatcattac aattgtttgg ttgaattttt
     6121 tacttgtaac tttaagtaaa ctcagtacat ctgaactagt tctactatca aggttacctg
     6181 ttggttcatc agctagtata atagctggtt ttgttacaag tgctctagct atagcaacac
     6241 gttgctgttg accaccggaa agattacttg gcatatcata taatttttct tcaagagaaa
     6301 gcatatttat aatttcattt ataaaatcct tatctatggt ctctccatcc aatcctattg
     6361 gtaagataat attttcgtat acattaagta aaggaacaag attataattt tgaaatataa
     6421 aacctatatt tctgcgtcta aagattgtaa gctgttcatc agtcatagtc gataactcct
     6481 taccctttat aataaccttt ccagatgtag gattatctaa tcctcccatc atatttaaca
     6541 atgtggattt gccactccca gatgtaccta caatagaaat aaattcgcct ttttcaatat
     6601 atagagttac actatcaaga gcttttgtaa tgtttgaatt ttgtccataa tatttcttta
     6661 aattaattac ttgtaaaata ctcataataa tacctcctta acatcttgta aatacagtat
     6721 aaaaccaaaa tgttgcagaa tcattacaaa tgaaatattt aaaaatttat ttattaggta
     6781 agaagataga aaaagtcgac ccttttccaa tctctgatgt aactttaata tagccacctt
     6841 gaagagatat aatttttctt gaaagataga gtcctattcc aataccttct atgtcatgta
     6901 catcttcttc acgataaaat cttttaaaga tttctgcttg atgactttct gatattcctt
     6961 ttccattatc aataatatct atttttgtat acataaccca acattctgta gttacacgta
     7021 tcttaccatt agaatttgtg tattttacag cattatctaa tatattaaaa atagcttcag
     7081 aagtccattt cttgtcatga attatatata aatttgggtc acaatcaact gtaacttcaa
     7141 tattcttttt ttcagcatca aacaaaattc cactaagtgc tgtagcaatt gtttcataaa
     7201 ttgaattttt ctttttagat agtgtgatta tacctgtctc tagtctggag gtttttacca
     7261 tagattgcat taaaaaatct aatttatcta attggttttc catatccatt aaaaaatcat
     7321 tttgaatttc tgttttaaat gtttgattta gtagagtagt attaatcatt tttaaatttg
     7381 caataggagt tttaatttga tgagatatat cagaaactag ttcttgtaaa tcagctttct
     7441 ctatagcaat actattgcga ttttcttgaa taacttcata cattctaatt aatctgtgat
     7501 tgatacgtga taaaatagtt tcttcttcta attcaatttc aatattgtct tcactattca
     7561 tcatactatc taaagtgtga catattttag aacaaaatat aactattttt ttatgaagta
     7621 aaaatattat taatgctata aatccacaaa gtactattgt atatattaat aatagagtaa
     7681 ctagttttaa atcatcagtt attatataaa ttgagatatt aaataaaata gctgtaaaaa
     7741 ttataccaat agcaattatt aaataaatag aactacaaga aaatttttta aaattcattt
     7801 ctttcaccta accacatata tcccatgcca tataccgttt taatatactg aagattgtct
     7861 ttttcaattt tgcttctaat cctacttata acagatgtta aagtatgttc atctacaaag
     7921 ttagcatcta tatcccagag tttttctaaa agtactttcc ttgttaaaat atttttagga
     7981 ttttcagtaa gcagttttaa agtacgatat tctaatggtg taaatataat ttgatttcca
     8041 tttatattgg cagataactc agaaaagtta atttcaagat atccatccct atagtaatct
     8101 tgttttgttt ttgttgtaaa atgatttaaa aaagctttaa ctttctgttt aaatacattg
     8161 atattaaatg gttttgttat ataatccaaa gctccaagtt catagccttt aatcatatca
     8221 cattccatat cattagcagt caaaaaaatt attggtatat tgtaattttc ctttatttca
     8281 ttacataatt caaacccact tttatctggc aaattaatat ccaaaattat taaatcaaat
     8341 tcacattcaa aaatagactt cattgcagat ttagctgtaa attttgaagt aattatatat
     8401 ccatcttcaa ttaggttata tgatagagtt ttatttaata tagaatcatc ttctactact
     8461 aatatttttt ccatacagtt actcctttta gtttaatatt ttataatgtc aattatagac
     8521 taaaaacatt gctgaattgt tataaacgag aaaaagtatt taaaagttta tatatttaat
     8581 aaagaacccg tcctatgata aattctaaga cagattcttt attaaataaa attatttcaa
     8641 ccaagatgga tttccaggta taaatattct tttaccaaat tttgaagtat actcatctaa
     8701 agctttttga ataagctcat tattaccctt aaatgaagta gtattcttta tagtttcgag
     8761 gttttggcta aatgggcaag cagatataca cataccacaa tctgtaccta aatatctcca
     8821 tttaatatag catgtctcat gttcaatcac ccaattatat tttccatcat caccaatttt
     8881 aatatcatta gaaagtgaat gagaagggca gttaaaagag cattttttac agaccttaca
     8941 aaaatcttct agtccaaaat caacatattc atcttccaaa agaggaatat ctgtagtaac
     9001 aacacctaat ctaagtctag agccataatc tttatttgta agtatagcat ttcttcctat
     9061 atctcctaaa ccagcatcac gagctataaa aacaggcatt actaaataat tagcatccat
     9121 atgatttcta gcatcatatc ctaaatttct tatatagtaa cttaaaatca tgccgactat
     9181 agatgcatcc acatagcact ttgatgtttc tataacttca catatcattg gagctctatt
     9241 tatcatttct ttatccatct cacatccaaa tacaattgca tacttatgag ttagattaac
     9301 ttcttcaccg taattttctt catgtctacc cctatttgta taaatatgat agtcttttaa
     9361 tttagttata ccaacgacag aagctccata ctgctttgca agacccttta ttttttttgt
     9421 cattgacttt gcatctacat ctactttagt atcagaaact tttccttcac ataaagattt
     9481 gatatcagat aaaaaatcga atgcactact tgccattgga gagtttaact cattgtaagt
     9541 cattgttcct tcactacata aatttggtct atttcttata ctatcatcaa tttctttttt
     9601 gtcaggattt ttcttataat agtcattata agcagtacta tcttttttgt aatttgctct
     9661 tgcaaacata gtatttctct catcaaccct tttcaattct attcccccta gtaaaaatat
     9721 atttaaaaaa tataacttta ttgttgtata atttaattat ataagaacaa cgttatattt
     9781 tcaatttaag tgtaattaaa ttggggaata tataattata tgcttgatta taatgttttt
     9841 attttgaaaa caaatttaga tacaatcttt ttagtgatat aaataaaatc aaaatatttt
     9901 tagtatgaca atgcaagaaa ataattattt atctaaaaaa tctaaaggag agtaaaattg
     9961 aacttattaa ttattgaaga tgatataaac ttaaatgaag gtctttttta tgcatttgag
    10021 aatgatggat ttaatgtatt taaggcttat acaaaacaag aaggattaaa tatatttaac
    10081 agtaaaaata tagattttat aatcttagac tgcaatcttc cagatggaga tggatttgat
    10141 gtatgtcaac taataaggga aaagtcagat ataccaatta taatgcttac tgcaagagac
    10201 tcagaaattg atgaagtaaa aggattagaa atagggcttg atgattatat tacaaaacca
    10261 ttttctttat ctgtattaaa ggcaagagtc aaagtagcaa taagaaaaaa atcgaataat
    10321 aaagtaatat attccaatgg aataaaattg gaccaaaagc tactaaaagt atataaaaat
    10381 aaagtatgtt tggaacttag ctctgtagaa tataagcttc taagttatct aattgaaaat
    10441 aaaggtcaaa tattattaaa ggaacaaatt ttacaccata tttgggacag cgaagaaaat
    10501 tatgttgatg agaatattgt gtctgtaaat ataagaagac ttagagtaaa agtagaggat
    10561 gacccatcta atccaaaata tataaaaaca gcttatggaa tgggttattt atggaatgag
    10621 gtagaataag atgtttatat tagcctttat aatatgtaca gttgttatat ttatagtatc
    10681 tactaaaata aatcaaaaaa gatatgatga attaagttta atgttaaatc aaatattgga
    10741 aggtaaagaa gttacttatc cagatacgaa ggatacaaga gcgtcaaaaa tttctcacca
    10801 agtaaaaaaa attaaggata tgatagaaat agaggtagaa cagtcaaagt tagaaaaaga
    10861 agcgattaag ggtttaatat caaatatgtc acatcaatta aaaactccac tttcaaatat
    10921 aaccatttat tgtgaacttc tggagaatat aaatatttcc acattacaga agaaagagtt
    10981 tttacaaaag atgaaaaatg aaacttttaa gattgactgg ttgttacaat ccctatttaa
    11041 aatgaccaaa ttagaggatg gagttataga atttgaagta gaagaactac taattaaaga
    11101 tactcttatt cagagtatca gtactatctt taataaagca gaagctaaaa atataagggt
    11161 aaatcttgaa ccattttcag atataaagtt agttcataat aaaaagtgga tcatagaagc
    11221 aattgtaaat gttttagaaa atgcaataaa atactcacca tcagatagca cgattactat
    11281 ttctgtaata aaaatggaac tgtatacgaa gattactatt aaagatgaag gaataggtat
    11341 tgatagtaga gaactaaatg atatttttaa aagattttac agaagtaaaa atgtggcaaa
    11401 ccaaaatggc acagggattg gtctatatct tacaagatta attttagaaa aagaaaatgg
    11461 aaacattata gtagaatcta aactaggaag tggatgttgt tttagcatct tcttacagaa
    11521 ttgtaagagt ttaaactaac tttgtaggat gagtgtaatt atcgtcactt acaatatttt
    11581 atagaacata tatctattga aaggagaaat taaatgagta tattagtgac gaataattta
    11641 gtaaaacact atggaaaaga tgagactaaa gtaaaagcct taaacggagt aagtatagaa
    11701 attgaacgag ctacatttac tgctatagtt ggaacttctg gttctggaaa aagcacattg
    11761 ttaaatatta taggtggatt agatagtcca acctcaggtg atgtaataat taaaggaaag
    11821 aacatatcta aaataggaaa aaaagatttg acagtattta gaagaagaaa tataggattt
    11881 atatttcaaa actacagttt aatgccagta ttaaacactt atgataatat agctcttcct
    11941 gttacattag ataaaggaaa ccatatagac catgaatata ttgagatgct gatgaagaca
    12001 ttgggaattt gggataagcg tttaaaattt ccaagcgaac tatctggtgg tcaacaacag
    12061 agggtagcaa ttgctagagc tttagcaaat aaacctgcac ttatattagc agatgaacca
    12121 acaggaaatc tcgatagtaa aactacaatg gaagtggtat gtttattaaa agaaagtagt
    12181 gcaaaattcc atcaaacgat acttatggta acacacaatg aaaatatagc acaagtatgc
    12241 gactcaatta tccatattga agatggagtt gtggtgaata ctggtggtga agtactatga
    12301 tgtctctagt aaaattttca attcaatata taaagcatta taaaaaacaa tctatatcta
    12361 tagttttaag catagtacta tcagtagcat tattgacggg aattggttct ttagttcata
    12421 gtgcagacaa aagtagaatt gaaaagataa gggaagatag tggagattat cattatttct
    12481 ataaagtaga taaggaacaa cttaataaaa ttaaagaaaa taaaaaatca aagaaataca
    12541 ctgtagatag acttggtgtt acaaaaccta aaaatcagat agatgaacct tttattatag
    12601 aatttttaaa tgctgattct tcatatttag acatgacagg tagaaaatta ttaaaaggaa
    12661 ctcttcctag taaagatgga gagatagtac ttgacacata ttctctaaac aatttaaatg
    12721 taaacaaaga aattggaaca gaaatcaatt tagatgggaa aatctataag ttatgtggta
    12781 ttttatcaag tctttcaaac ccagaaaccg ctatggaggg attcgtaaac gattatttaa
    12841 agcctaaaga aaaggattct tatatagttt atgtaaaata tgatgaaaat aaaaatatta
    12901 aaaagcaaaa ttataaattt atgaaaaaat tcaacatatc aaaagaaaaa gcttatataa
    12961 actggcctct ttcagttgaa tttggagtaa aaccaccaaa aaatatagaa aaatataata
    13021 tatttgagtt cttaaaaaat ttagaattaa atacaaatgc aattatatta cttgttggag
    13081 tgtttggtgc agcagcagta tatagtattt tccatgtttc aatacttcag agaatatcac
    13141 aatatggggt tttagaagta ttaggtgcta ataataaaca acttttattt ctactatttt
    13201 tagaattatt tttacttttt attgttggat ttcctatagg ttgcttccta ggtattggag
    13261 tagcgtctac aatatatgaa caatttccac atatattttt gagtagtgat attgttccag
    13321 gagcattttt tataagtgaa aaatctattt atttaggatt tttatttatc ttattattgt
    13381 taattttaat ttctgtacgt gttgtacatc agttgaataa atattctagt atagaatcta
    13441 tgaaaaactt taagattact aatacgaaaa agagaatatt agaaagtgta aaacattcta
    13501 acatgacaaa agtattatct cataaataca tgactcaaaa aaagggatta tttgttggaa
    13561 ttttgttttc attatcactt ggtggaatta tatttctatg ttctagttac tctatacagc
    13621 ttactaaaaa caataatgaa cttacaatga aagctgatga tggtttaaat tctgactacc
    13681 aaataactat gcaaacaaca gattttgatg taggtatacc aaaagataaa atttcaagat
    13741 taaacaatat tgagggaata tcaaaagtct atccagtaag atatttattt ggagctatgc
    13801 aaattaaaga agaacaacta ttttggaaaa atttctttaa acctcttgaa aaagattaca
    13861 ggataaagac tttttttgat ggtatttgta caaagcaaaa tgaaggcggc tatcttttaa
    13921 aaacaaatat ttatggttat aacaatggca tgttagaagg gttaaattct tatattatag
    13981 atggggagat taatacccat gatatggtta aaaataataa ggttatagtt agactaccta
    14041 tggatggaac cgggatgtat gatgcagttg atataaatcc aggagataca ataaaagtta
    14101 aagtgccaaa gataatgaaa ccaacaggtg aaattgttaa atttaaagaa gaaaataaga
    14161 atgattatac aacaaaagaa tttgtagttg ctgctactgt aaaacgtgtg atggcaaata
    14221 acatatattt cattggtgat tatggtatgg atatagttat gacgaatgga caaatgaaaa
    14281 ataactttaa tatcgaaaat tataattcag taagtataaa aaagacagaa gatagtaata
    14341 gtgtggaatt gtctgaggac ataaaaagtg ttgtaagtga tgtaaaaaga tgtatcgtaa
    14401 cagattatac tattgctatt gaaaaaaata atacctactt aaatcaaaaa ctcttattta
    14461 tatatggtat tgtatttgta cttcttgcaa taagtttatt ccatataatc aatacagtaa
    14521 gctacttaat tttttcaaga agacatgagt ttggaatttt aagagctatg ggtattacag
    14581 ataataagtt ttcacttatg atgataagag aagggttttt atatggattc tatgcaagta
    14641 ttataatggt aattggaagt gttattggac aatttatgat ttattttatg gtaaaacgtg
    14701 tatatttata tataaatcca attcttaaaa taaatgcacc tctatacata ggtatgatta
    14761 ttttaaatat cactattagt atagttgctg taattattcc agtaagacaa attttgaaga
    14821 gtgatattat ttcagaaata aatcagaaat aaataagagt tagagttata taaattttta
    14881 agattatata aatttctgac aatcttgttt aaattattta aatatatctt atctgtgaac
    14941 attaaataaa cctcttaata gatataactc ttgaaaagtc atatctatta agaggtttta
    15001 ataaattttt atgttaatat atataatcca aaaaatttaa atcttattaa aacatactat
    15061 tttgtatagt tatcgaaata tccttgtatg taaataatag gagtaccctt atctccactt
    15121 ccagatgtta aatctgctaa agaacctata aggtctgtta atcttcttgg agtagtacct
    15181 tgagaaacca tgtttcctgt taaatcatca gatttattat ctttttcaac tatgtatttt
    15241 gaaattgctt ctttcaattc atcaccagat aagtcagcaa aatcattatc tgctaagtat
    15301 tttaatttta cttcgtttgg agttccttct aatccttttg tatatgcagg agatactact
    15361 gggtcagcta attcccatat tttacctaca gggtctttaa aagctccatc tccataaacc
    15421 attacttcta catctttccc tgtgatttct tttatattac cttggatttt gtttacaact
    15481 tcatcacaat ttatagggaa taattttact gtttcttctg tagctttgtt tgaacctaaa
    15541 agtccatact ggtcattata accactacca tctacacttg ctgtcattat atcatcaagt
    15601 gaatatactt ttttagctcc attttgtctt aaaattctct ttgttctttg tcttgtatgt
    15661 atatcacaag ttaaaatact tgttgtatag ttaagaatag tttttggatt gtttgcaaat
    15721 actatttcta cctctgcacc acaatccttg attaaattct tataataatc aacataatct
    15781 acaccagtaa atgtatgttt attatatcca aatagttctc tgtatttttc ttctgttaaa
    15841 acatctgacc aaggatttat tcccttatca tcaagttcat caatacttat taagtggttt
    15901 ccaacttcgt cagaaggata gcttaacatt aatacaactt ttctacatcc ttttgcaatt
    15961 ccttttaaac atatagcaaa acgatttcta cttaaaattg ggaatataac cccaacagta
    16021 tcatctccaa atttatcttt tacatctttt gcaatattgt caacatgagc atagtttccc
    16081 tgtgctctag ctacaacagc ttctgtaacg gcaattacat ctttgtctct aacttcaaag
    16141 ttttcacttt ttgatgcatt taaaacagaa tcaacaacta ttttaactaa gtcatctcct
    16201 tgtcttataa taggtgctct aacccctctt gaaacagttc caactaatct atccattatc
    16261 taaaatcctc ctaaagaatt aatattgtca tattagtacc atgtatatta taattcaaat
    16321 ataattataa gtaaaataaa taatactaat atttgacata aggagtaatt atatatgaat
    16381 ataaaacttg aactttataa ggtttttaat gcagttgtaa ataataagag tttttctctt
    16441 gctgctaagg aattgtttat gtcacagcca gctgtaagtc agtcaataaa gcaactagaa
    16501 gaacaattag atactttatt attttataga aacaataagg gagtaaaatt aactccagag
    16561 gggaaaattc taagtgaaca tgtcactact gcattaaagt taatatcatc aggagaagat
    16621 agaataaata aatttaaaaa attagaatat ggttctctaa aaataggagt aggagataca
    16681 gctgcacgtt ttttcttgtt aaaatattta gaaatttttc ataagaagta tccacatata
    16741 catgtatcta caataaatag aacgagtaga gagcttatat ctttactcaa agatgggaat
    16801 atagatatag caattataaa catgccaata gaagatgata cactaaatat tgttgagtgt
    16861 attgaaatac atgatatatt tgtgtgtgca aatgattaca tagaatacaa agggagaaaa
    16921 atatcattag aagaattaaa tacattgcct ttaataatgc tagaaaataa agcaaattca
    16981 agactttatg taaatgaata ttttttatca aaaggtataa aactaaaccc ggatatagaa
    17041 ttgggttctc atgaattgct tttagaattt gcgtatataa atctaggagt atcttgtgtt
    17101 atagaagaat ttagcataga ttatttggaa aatgggaaat tgtttaaatt agatataaaa
    17161 gagccaatac ctaagagaaa tataggttac tgccatttaa aagatatatc tttatcctta
    17221 gcaaccaaag aatttttaag catgatatct aataacatat aatttagaat acatatttca
    17281 caagtaattt aatataaaaa tagagataag ttcgtaaatt tgaaagattc atatagtaat
    17341 tagtttttat taaaaataaa aataaggtta atttgagaac atttatataa ttaatatcgt
    17401 attttaaata tgaacagtat atttagatta atgtataaag ggggattaga aatgtctgat
    17461 tctgaatatc ctcaaatgaa aaccttagaa actagaaatt gtatactaag acctgcatca
    17521 ttagatgatg tcgaagattt ttttgactgt tataaaaata aaatagtagt aaaacattta
    17581 ccatttagtg agcataaatc tttagaagat acaagaaagt ttataaaatc attttttcta
    17641 aatccctata aaaaagggaa aattggacat tttgctatag tatataaaaa agacaataaa
    17701 gttattggga atatggggtt taataatata aatcctaaag cattagaagc agaaataggt
    17761 atatgtataa atccaagtta ttgggggcat gattttgcta ctgagataac aaaaagagta
    17821 atacaatatg gatttagaga actaaactta aataaaataa ttgcaataac ctatgaagaa
    17881 aaccaaaatt ctacaaaatc attagattta ttaggattta aatgtacagg aaagtatatc
    17941 aaaaaaattc atactggaaa tactgtaaaa aatgttcctt gttatcaata tgaattgaaa
    18001 aaacaataga tataatcatt taatatttat gctagttatt taataatgat tgttttctaa
    18061 ataaaatttc aataaagtgg ttgcctcaaa atatagataa gcctgttttt tattgtaaaa
    18121 atactgaaaa tgcatttgat tttatacaaa agagagtgtc tcatgaacca aaaagtttat
    18181 gagacactct ctttgaataa atcgtatttt aattaaaata gtatatatac ttaaaatatt
    18241 aattttttat tagttctcta aatttttaaa tccttctcca agaacttcat gcacatctgt
    18301 aacaataaca aatgcagtag ggtcagttct tttaactaat ctctttagat aaagttcttg
    18361 ttttttagaa actacgacta atagaacttc tttattttct ctagtgtatc cacctttacc
    18421 atcaagaata gtaagacctc tatcaagttc ttcagttata gcttgtctta aactttcagg
    18481 ctcatcagaa ataataaaaa atgcttttga atggtcaaat ccttccatta tcatatctgc
    18541 tatttttatt aatacacaaa gtgctatacc agagtaaagt gcagtttcta aactacgatt
    18601 tactatacca gaagatataa caaccattcc atctaggcat gacatgaatt ttgtagcttt
    18661 tattcctggg aacttcttac taagtatgag tgcaattaag tctgttcctc cagtagaagc
    18721 atctgattta aacattattc ctaaacctat accaactagg attcctcctg caatagaaga
    18781 caataataag tcattagtaa atcttagctt tgataatgga tctgttaatt ggactatagc
    18841 tgaaaataca actgttccaa ataaagtttt taatgaattt tttgtaccca taattctaaa
    18901 agcaagtatc actaatggga cacctataat taacatgata gctgatactg gaagtcctgt
    18961 aaccttgttt agtactaatg atagaccact aagtcctcca ggagcaattg tatgtggttt
    19021 taaaaacata tttataccaa tactcattga tatacaaccc aaaaatagtc caagcatttc
    19081 aagtaaaatt ggcgactttt gttttttcat ataaacacct ccatgaactt gtcacaattt
    19141 aatattataa catatttgac cagtttatat tatataaaat ttcttgatat aagcaatttc
    19201 aagtatttca ttattcttaa tatttttgaa tttttatata gtgtttttat atctttactg
    19261 atgttgttac actcctatga agttttttat atattataat tttttaattc ttttataaag
    19321 tatcccctat aaagatggtt ctatgaaaaa tttctctata ctgtttaacg tatatagaaa
    19381 ttttgtgtat aattataagg tatttaatgg aaaattttat cttactttta tatgttatca
    19441 aaaggattta ttaaatacaa cggacagctt gataacatat aattatataa atataattta
    19501 tgtagatagt tgtattttta aataaatttt tacatgtcaa taattattgt ctattttata
    19561 taagcaacaa tcaaactaaa ataaaagttt aagaaaagag ttgattaaat gcataaacct
    19621 agttataaaa cggtatttca ttcatacatt atttttttta tgatttttat ttgcttaatc
    19681 ctgacagtaa taggtatttt cttatcactt attactattc agaaaccaaa tggggaattt
    19741 attcgtagtg actggccgaa aaaatttaca gaaaacttta atgaacaaat cgtatttata
    19801 gataataagc cacaagtaaa acagtctgga ttagaaaagc ttcgagaaaa tcaattgtgg
    19861 ttgcaaattt tagattctag tggaaataga gtttatgggt ttttagaacc aaaagagcat
    19921 aaagattatt attctaattc tgaattatta acattagcta accaaaataa ttcaagtaat
    19981 ggaactgtat actttaatgc aattaaaaat aataaaaatg actatattta tattatatat
    20041 tttccattaa atatatctaa agtgaatatg ttttttgatg gaagtaaatt tgtaggtggt
    20101 aagtcaattg tattttcaat catagggatt atatttttaa taattttgct ttctggaatt
    20161 gcttatggtt actgggtaac aaagtgcata tcaaatatta catctgctgt tagtgatatt
    20221 tcaaaacgaa gctatcttcc agttaatatt aaaggtgttt ttactgatat atatgatagt
    20281 ttaaatattt taaatgaaga aattcataga agtgatgaga tacaaaataa aactgatatt
    20341 atgagagaag agtggatttc aaatataact catgatttaa aaactccact ttcaccaata
    20401 aaagggtatg cagagcttct tacagatagt agtattaatt attcacctga aaaagtatat
    20461 aaatatgctt caatcatgtt aaagaacata gaatatacaa atacacttat aaacgattta
    20521 aaattaacct atcaattaaa aaatggaata atccctttaa ataaaaataa ctatgattta
    20581 gtaagactct tgaaagaatt atccattgat attttaaatt atccagaata tgaatcaaat
    20641 atagttgaat ttaagagtaa tatagataat attaactttg attttgatta tacactatta
    20701 aaacgagctt ttagtaatct tattgtcaat gcttttacat atggagataa aaatactaaa
    20761 gtttgtgtac atataacatt aaatgaaaga attgaaatca ttatatctga taatggaaag
    20821 gggatgtcaa gagaagaggc atcaaatttg tttaatcggt attatagagg aagcaaaaca
    20881 aaaatagaag gaactggttt aggactagca ataacaaagc aaattattga agttcatgga
    20941 gggaatatat ctgttgaaag tatacttgga ttgggtagtt cattttatat catctttcct
    21001 atttaattaa ggttaattaa ggtcaaataa aaaatatatt aaggttgata gattattata
    21061 tttatatagt agtttatcaa tcttttttat ttaaggaggt gatttgatta aaaatattat
    21121 aaatagaatg ataaaagtat ggaggagtct attagagatt caatttggat taaagtataa
    21181 aaatattata ctgaaatatc ttagataaag gaagaaggtg taaatttgaa aattatatta
    21241 aagtatattt tcacaaatat taaagaacga aaaattagaa cagcagttat gttattgtcc
    21301 atagttttat caacagtttt actttttgtt tcattctcga ttggattatc ttatgaaagt
    21361 gcacaaagaa aaatggcaaa aggtatgtct ggtacagcga ctatatctgt ccaaagtaaa
    21421 aatccagata tattaaccaa tttagaagac atacctgatt taaatgctat aaaatcaaag
    21481 gttggtgtat tggaaagctc tgctatttat aataaggggg gatactatga agaatttagt
    21541 cttatagcag cagacttaag tcaactaaat aaaatcaaca agccacgatt agaaaatggc
    21601 gatagtatta ctgatttttc tggtgataaa attattcttc caaaccgttt tacatcaaaa
    21661 tataaaataa aaaaaggtga ttctattact ttacagattt atggaaagtc ttatactttt
    21721 caagttagtg atattgcatc ttatgataca gtatttttga ggcatacaag aggtgtgaat
    21781 gcactgctac ctaaagagac tctatcaaaa attataaata aaggcagtgg atatacaaga
    21841 gttcttatag agtcagaaga agaaatgaca gaaaatctag taaataaatt atccgaagaa
    21901 ctttctactg aaaaatatac agtatcaaat actataaatg aaactaagat aatttcagat
    21961 gctagacaaa agataatgcc tttcttttta ataagttttt ttgcattaac ccttagtata
    22021 tttatcatat acagcagtta taaggttatt acattagaac gtcttccttt tattggaaca
    22081 tttagaagta ttggtgccaa tgaaaaaact gtaactcata tattgatgct tgaaagtata
    22141 ttatacggaa gtataggagg attaattgct attcctattg gtgtagttgt tttaaattta
    22201 atgcttcatg ggttaggaaa ttctctcgaa caaggaatat caattccaac agtaatatca
    22261 cccattggag tgattatatc tgttatagta gcaataatcg tttctttatt tagtgcatat
    22321 atacctgtta aaaaagctag ccatttgcca ataaaaaata ttgtactagg aacagtagaa
    22381 gaaaagaatg tttcaaatcg ttctatactt tttataggaa gtataatgtt tattttatct
    22441 gttttacttc caagaatatc ccctgaaaat acattgtatc tagctggcgg attttcatta
    22501 ctaggattaa ttgtagctac tattgtgttg attcctctta ttactgatat catgtctata
    22561 gtttttgagt ttgtttataa aaatatttta gggaatgaag gtaaattagc tgctagaaat
    22621 atgaaaaata acaaaaatat aatacagaat atcactttac tatttataag tatttctgca
    22681 gttattgcaa taagcgttgt tggtaacttt gtcaaaactt atataacaga tgtgtttcgt
    22741 gacgcagaat tacaaggttt tgcagatgga aagatgaatg aagagtttat tgaagatgtt
    22801 agacatatgg atggaattaa aaacatatta cctctttatg ttatgaataa tgagatttct
    22861 ggtaatggtg taacattatc aagactagag ggtacagata atatagaact atataactct
    22921 atgttcgcaa tcaattatac aaattttgag ataaaaaaac aagtaacaga agcttttaag
    22981 gataaacgtt ctgttatttt gaatgaagat actctaaaaa aagtagggtt atctatagga
    23041 gatacaataa ctctatctaa agataagtat gatttttctt ataaaatagt aggtagtttt
    23101 aaatctcgtg caaatgatgt agaagctgtt ataccatcac attatgcagt taatgatttt
    23161 gataaaacta attatggttt tttagtatat acagcagtca atccagatgc tgttatgatt
    23221 cagataaggg atttatttgg agatacatat aattggagtc gtacagttga agaattttat
    23281 aatgattctt taaatactat aagtagtttt ttgtctccta tgaataagat gacctatttc
    23341 atttttcttc ttgctacagt aggtattatt aataatctac tgattaatta tattcaaaaa
    23401 agacgttcta tagctatgta taaatctata ggactaagta ataaacaaaa tataaaagtg
    23461 acactgattg aagggtttac ttcaggttta cttggagctg ttattggtat tgtaatatct
    23521 atacttgaga ttcaaactat ctttatagtt gcaggaccaa aaatttcaat gaaaccagat
    23581 ttagatttta aaacatttat tattgttggg ttattgggaa ttatagtaac tttaataggt
    23641 tcgatagtgc ctattataaa agggaagaaa atgaaactga tagaagaaat taagtttgaa
    23701 taattatgga ggttaatatg aaaattacac ataatagttt agctgtagaa gcaaaaaata
    23761 ttatcaaaga atataaaatt ggaaatacaa caactcgagt gttaaaagag gtgtctttac
    23821 aagtaatgaa aggtgaattt gtttctatca tgggtcaatc tggttctggt aaaagtactc
    23881 ttttatatat tttaggtggt ttagatactc caacgagtgg aaaagtgtat atgaatggag
    23941 ctgatatatc acattttaat gatgagaaaa tgagcataat acgtcgtaga aatataggat
    24001 tcgtatttca attttataac ttaataccaa atttaaatgt ggaggaaaat ataatgcttc
    24061 ctttgctatt agatggtaaa aatttaaaag attataaaaa tcaattagat gaaatcttat
    24121 atatagttgg tttaactgat agacgtaaac atacaccacg agagttatct ggtggtcagc
    24181 aacaacgtgt tgctatagct cgagctttaa ttggaaaacc agagatttta tttgctgatg
    24241 agcctactgg aaatttagat agtaaaacag ggatagaaat aatagattta ctggataaaa
    24301 taaacaggga caatggacaa actataatta tggtaacaca ctcaccagaa gcagccaaaa
    24361 gtagtagtcg tactattaca gtttcagatg gtttaattgt ataagggcta attattgtat
    24421 ttgagataat catttaaagt atataaattt tgcatatatg aattggacat tatcaaatgt
    24481 gttataatat taaaacaaca aataaaatta ttatattttt tagaataata attttatttg
    24541 tttactaagt ttatgatagg gtatttattt aaggtaataa aatattatat aaaatatttt
    24601 aaagccttag gtaatattag gtagaagttt tagtaaaata tgcaaaaact aattgattat
    24661 gtaccttgta tgggtatata atagatattg ttttaataaa atatttgaaa gggaatgatt
    24721 tgatgaaaat tactttacca gaaacagcta tcgatacttt aaaaagtatc ttaaaagaca
    24781 atcaagataa acctaataat ataagagtat actttgcagg tgttggttgt ggaggtcctt
    24841 cttttggatt agctttagat gagaaaaaag aagatgactt aacttatgaa gttggagaat
    24901 tacaatttgt aatgagctct gatgaatata gtcagtatgg agatataata atagaagata
    24961 caggatttgg atttagagtt attcctgaaa atatgaaaga tcaaggtgga ggaggatgct
    25021 ctggttgttc agggtgccac taagattaag tttttagtaa gtttagaact ctctttatag
    25081 aaataaattt ctattttaag agagttcttt ttattttttg gtgagataat gtataataaa
    25141 aatgtatttt actaattttt tatatgtaag gagaattatt gtgagaagaa gacgaaaaaa
    25201 aggtgcagat gaaaagcttt taagttatac aaaatatgtt ttaagagacg atatagacaa
    25261 attaaaaggc aagtggaatt taaagtttag aaatgataat ccaatacatg ttgaatttgg
    25321 gactggaaaa ggtaagttta taacaacttt agctaagcag aaccctgaca taaattatat
    25381 tgccatggaa cttaaggaag aagttctttt aaaggctgtt gaaaaagcag atgcttctaa
    25441 tttgaataat atactatttt tatggggaga tgtaagcaat attttggatt actttgaagc
    25501 taaagaatta tcgagaattt atataaattt ctgtgaccca tggcctaaaa atagatggag
    25561 taaaagaaga ctcactcatt ctggattttt agaaatgtac aatagggttt tggaagatga
    25621 tggcgaaatt cactttaaaa cagataatga aaaattattc gagtttagtt taaatgaaat
    25681 tgctgcaaat aattggctat taaagaatat ttctcttgat ttaggaaata gtgagtatga
    25741 aaataatgtt acaacagagt atgaagacaa atttatgtca caaggtatgc gaatatttag
    25801 atgtgaagct aaaaaacgta actagtaata tgtttataag tttaatagta gaaatttatt
    25861 ttaatttata ttaataaagt gtacgaaagt aggcatatat aacaaggaaa cagaaccctc
    25921 tcatagaaat taaattatta taataaaaaa attatgtggg agggtactat gaatttagct
    25981 aatttaatac catttattat ttctgcagca gctttaagtc caataaattc aaatgataag
    26041 gttttgagcg aaaatcaact taaagatatt tttcctgatg caaatttaag agcagttgta
    26101 aaaagatata taaacccaga tgagatgact ataagcaata taaaggcttt agatggagaa
    26161 ttttatgcaa ctggtgagag catttcaaat ttaaaaggaa tatcatatct tgaaaatgta
    26221 gacaatttta ttttttggaa caataatata aaagagattc ctaaagaagc tttaagttta
    26281 aaagatatgg attcaataaa tcttgccaat aattatttga ttgatgacag tgtagtaaat
    26341 tctctttctc ataatggtgt ggatgtaaat tgcgatttaa actttatcga tacaaaagat
    26401 aatcagtata aactggactc taaatatcat aatgttaata tagcaaaagg tgaaagtatt
    26461 gatttgagaa aaataattac gaagaaaatt gatagctatt ataaatactg ggaagttact
    26521 gataaccttc caaaagattt agattttatt gtatctgtaa gtgataagag tgtactttca
    26581 tgtgaggata tgataatcaa aggtaataaa acaggtcaag cagtagtaaa agttatacta
    26641 aacgacaaaa atgatttaaa tacatctcaa gaagttctaa ttactgtcaa tgtaaaataa
    26701 tctgtcaact tatttagtca ttctattttc aatttaagtg tataatcaaa ataaaggttg
    26761 ctagtaacag aacactaata ttagcgcttt gttactagca acctttaaat tttatagaaa
    26821 tagattattt aaaaaattcc atttcttctg ttagtagccc atattcctat taaaattatc
    26881 agtgaatata ttatatacaa aactattctt atatactgga catctaatcc aaaaaattca
    26941 atgttactct gtgaggtatg caacatcaca aggactgcag atataccaat aagcaaagga
    27001 tttattacta tttgcttatc agttcttaaa atcattattt tacccatcca aattattgat
    27061 aatataaaaa ataaaatttt aagaccaaat tctaatgtat ctaccaaagc gaaaacatcc
    27121 cttcatttat aataaatttt tcagctcaac aaaatataaa accctaagaa caacttttaa
    27181 atctataata atatagcttg attaagcact ataaaataag taccataaaa ttttatctac
    27241 attaatatat actacactat acttcaaatt tcttaaacat aaattaatga aattaacaaa
    27301 aatttattca taagcacata aagatatatt tcattattta aatgcatatc attatatata
    27361 cagttttagg aggtgaggta tatggaatct aaatttttta attggtacac tcaatcctta
    27421 gggggaactt ttggcattat atcatgtata tgcgcctact taaatggata catgtttgtt
    27481 tatacaaata gtgctagcaa atactttgaa agtataggat ttctgggaat aatttcaagt
    27541 tatacactac ttccactttg tatgttgact ttgatttttg gagttatata ttcttatact
    27601 ccagaaacat cagacaaaag tatttttaat ataccttttt ttactttaaa taaaagatta
    27661 atacttacta cagtagtttt tggttttata ggtgctagag tttactttat aattcctgcg
    27721 attttaataa cttttaacct atatagtaat gttttaatag ctaaattaaa gtatatccta
    27781 tgtaagaata aaggaaatga tactaatttg aaagaaagtg aagtctaaat tgcataatat
    27841 tggtatttgt cttaaaaatt cccgaggtat taatttgtat tcttttgtat tttgcaaaaa
    27901 atattgaatt ttattcattt tatacttata atttatatta ataatggaaa attaaaacaa
    27961 atataagtta ctggttgtta atttaaaatt aaaagatata ttgatgtgtt gattgtctag
    28021 tcagcacttt ttcttttata aatttgtttt ttgtgatata atattagcac caggtaataa
    28081 tacaaggggg taacaaaatg aacatcaatg aaattaagga attattaaag gccatagact
    28141 caactaactt agaatatgta aaacttgaaa gtagcgattt aagattagaa gtatctaaaa
    28201 aagctcaagc tacaagttca cctgttttaa gtgtacaaca agaatcagta gtggatcttt
    28261 ctttagaaaa gccagttgta aacgatacac ctgtcacatc taatgagaat ctatcagtag
    28321 tggtagctcc tttgatggga acattctatg attctccaag tccagatgca gataattttg
    28381 ttaaggttgg cgatgtagtt gaagaaggcg acactttatg tatattagaa gcaatgaagc
    28441 ttatgaatga gataacaagt gaaattaaag gtgaaataat tgaagtatta gttagtaatg
    28501 aagaactagt agaatataat caacctttat ttaagataaa accattgtag taggaggtat
    28561 ttaagttgat aaaaaaaata ctagttgcaa atagaggcga tatagcagtt agaatcatca
    28621 gaacatgtaa agaactaggt ataaaaactg ttgctatcta ttctgaaata gacaaagatt
    28681 gttttcatag atatatagct gatgaatcta tttgtatagg accaaataat ataagtaaga
    28741 gttataataa tatagaaaat ataatatacc ttgctcttaa attaaagtgt gatgctattc
    28801 atccaggctt tggattttta tcagagaatc ccgaatttgc aaaacaatgt gaagagaaca
    28861 atattatttt tataggtcca acaagagaac aaatgatttt gatgggagat aaatctagag
    28921 caagagaaac tatgatggaa ttaaatatcc ctgttgttcc tggttcagaa tctgttttga
    28981 aaactaagga agaagcttta gaagttgcaa gagaaattgg atatcctgtt atgataaaag
    29041 cctcaagtgg aggcggtgga aaaggtatga gaatcgttag aaaagaagaa gaactttttt
    29101 caaattttga tatggcaagt tcagaagctt tagcagcatt ttctaatagt gatttatata
    29161 tggaaaaatt tatagaaaat cctagacata tagaagtaca agtatttgga gataaacatt
    29221 caaatgcaat acatttaggt gatagagatt gttctatgca aaggcgaaat caaaaagtaa
    29281 tagaagaatc cttaagccca tacctttcag atgaagaaag acaaaaactt cataaaattg
    29341 cagtagatat agtaaaaggt gtaggttata taggtgcagg tactattgag tttatagttg
    29401 acaaagataa aaacttttat tttatagaga tgaatacaag aatacaagtt gaacatccag
    29461 ttacagagat ggtaacaaat cttgacctta taaaacttca aatatctata gctaatgggg
    29521 ataaaattcc ttttaaacaa gaagatatta cttttagagg tcatgctatt gaatgtagaa
    29581 taaatgcaga agattcatca aagaattttg ctccatcacc aggtaagata gaatcattaa
    29641 atttacctgg aggttttggt gttagatttg atacttttgt atatgcagga tatactatac
    29701 cacctttata tgattctatg ataggtaagt taatatgctg ggctgaaact agagaagaat
    29761 gtataagtag aatatataga gctctagacg aaattattgt agaaggtatt aatacgaatg
    29821 tagaatttca aaaggcactt gtaacaagtg aggagtttag agaagatact catcatacca
    29881 aatttataga agatgtcttt atgaagaaag aatttgctac attataagga gtaaatagat
    29941 tatgataaaa aaatttttat catcaaaaga atctaagtat gttacgattt ctttagatga
    30001 caactttaaa aagaatagtg tagatgataa attttggacc tattgcaaag gttgtgatag
    30061 tcatgtattt agaaaggaca tagaagaaaa ttcatttgta tgtccaaaat gttcaagaca
    30121 ttatggctta agagcaagaa aaagaataaa tttattgatt gataaaggaa cttttatgga
    30181 gtttaattct gatgtagaat ttcaaaatcc actaaatttt cctaaataca aagaaaaagt
    30241 agactcttat aaggaaaaaa caaaagagtc agaagctgtt gtcacaggtt atggaagaat
    30301 aaatggaatt aaaacagtta tttgtgtaat gaatcctgat tttatgatgg gaagtatggg
    30361 ttctattgtt ggagaaaaaa taacttattc aatagagtat gctgctgaaa ataatttacc
    30421 tatcattatt tgttctgctt cgggaggagc tagaatgcaa gaaggtatgg tatctcttat
    30481 gcagatggca aagacttctc aagcgctttc taaacttgaa gaaaaatcat taccatatat
    30541 atcagttcta actgacccta ctactggagg agtaactgct agttttgcta tgcttggaga
    30601 tattataatt tctgagccaa atactttgat aggttttgca ggtccaagag taatagaaca
    30661 aacaataaat caaaaacttc ctgaaggatt tcaaacttct gagtttttac ttgaaaaagg
    30721 ttttattgac atgatagtag atagaagaaa aatgaaggaa gtattatatc aaattcttgc
    30781 tatgcacaag aaatagggca taaaggagga actgttttga ttgaaaataa tagtgaaaaa
    30841 ataaaagtct tagaaaatga tattaaacaa ttaataatta tttctaaggc aaataatata
    30901 gatttaagtg ataaaataaa ttcacttaat gagaaattgg agaagcttaa agaagatgct
    30961 ttttcacatt tatcaccata tgaaaaggta atgctaagta gagatataaa aagacctacg
    31021 accttggaat acatagaaca tatttgttct aattttttag agcttcatgg tgataggctt
    31081 tttaaagatg acccttcgat agttggagga attggtcaga taggaaaatt taatgttact
    31141 atagttggtc accaaaaagg aagagatact aaagaaaata taaaaagaaa ttttggaatg
    31201 cctcatccag agggatatag aaaagcatta agacttatga aacaagctga aaaatttaat
    31261 agacctataa taacgtttat cgatacatca ggagcttttt gtggtctaga agcggaagaa
    31321 agaggacaag gagaagctat tgcaagaaac ttacttgaaa tgagtaaact ttctgttcca
    31381 gtaatcactt ttgtaattgg tgaaggtggt agtggaggag ctttgggtat tggagtagga
    31441 aatgacgttt gtatgttaga acattcagtt tattctgtga tttctccaga aggactttct
    31501 agtatcctat ttaaagattc gtctaaagct aaagaggctt gtgatgttat gaaacttaca
    31561 agtaatgatt tatatgattt aaaaataata gataagatta taaaagaacc tcttggagga
    31621 gcacaaaaag atgtagaggc tgtttcaaaa gaaataaaag cttatatttt agaaagatta
    31681 aatcattaca aagacatgga taaagaggaa attatagctc aaagatatac taaatttaga
    31741 aatattggaa aatgcttata gaatataaag aaatttgtag ttttttcatt ataaaatggt
    31801 taaaaaaaat aaaggttttc ttaaaatgat tatagaagta tatttttata aaagtatact
    31861 ttaaatgatg attgtaatta gtaaatggca attatgattt agtactttag gtaatggata
    31921 ctttaacaaa ttaacaagga gatgttgaaa tggaagtact aaaagtttca tcaaaatcta
    31981 atcctaattc tgtagctgga gccttggctg gagtactgag agaacgaggc attgcagaaa
    32041 tacaagctat aggagctgga gctctaaatc aagcgataaa atcaattgca atagcaagag
    32101 gttttgttgc acctagtgga atggatttag tttgcattcc cgcatttact gatatagaaa
    32161 tcgaaggtga taagaaaaca gccattaaac ttataattga acctagataa tatatctctt
    32221 tattagaatt taattaagtt ttaaaggttg gctatagaaa taggacacta tagtgaacct
    32281 tttttacttt atctgtttta ttttatgtaa cttttaatgt ataatcttaa gtaaacaaat
    32341 aagattggag gaaatataca ttatgaaatt aaaattattg gtagaagagt atgcagtatg
    32401 tagattaaat aatgatacta aaataccaac atggatagat acagagaggt tttactctat
    32461 aactagaaca gatgatgaat taagtattgt atgcttaaat aataatattc cttctgatgt
    32521 taagtcagaa aaggagtgga gaatacttaa gatattaggt cctttagact tttcacttgt
    32581 aggaatttta tctaagttaa gtagtttgtt agcagataat aaaataagca tatttgcaat
    32641 ttctacatat gatacagact atatattagt aaaagaaaaa gatatagaaa atgcttgtaa
    32701 aattttaaac tgtaatggat atgaagtaga gtagttaaat acttttacat gaggtttaac
    32761 ttatgattta tataatcgaa attttaaaaa taagtataat aaatattttt tgtttcatag
    32821 gaatatttat tgtatttgga ttgattttta gcattacaga aagtctaaat aataaattta
    32881 tcttttcatc ttttggaaaa attggaatta ttgtaactgg agctatagga acgtttgttc
    32941 atgaattgag tcatttgata atgtgtttga tttttatgca taaaataaat tcagttaagt
    33001 ttttcagacc aatagaaagt aaaaatgatg gtatactagg atatgtaagt catagttata
    33061 aaaaagataa tctttatcaa agtataggaa atttctttat aggaattgca cctattatag
    33121 gtggaacagt aatgattatt ttaattttta agatattatt gccagatagt tatatgaaag
    33181 tgactcaaaa tatagattta aatttatatg taagtatgat taataattta gacataagtg
    33241 gttttatata cattatttta ggcgatattt ctaattttat aagaataatg ttgttaacac
    33301 ctagtgtata ttcattaaag tattttatat ttatgttttt aatgtattct atatctacac
    33361 atatgtcact tagtagtgct gatttgaaaa atagtttaaa tggtttgagt tttatagtca
    33421 tagttatttt tgcaataagt ctaattacat acttatttgg atttgataac ttaaatatat
    33481 ccagtttaat agttaaatac aatatatttg taagtttatt tatggcaata gggcttattt
    33541 tttcaatagt aactcttata atatcctata ttttaagctt ggttagtcca tttaaaaact
    33601 ttagttaaat aaatacatat tttactaata tttggttttg ttaaaacata taaagatatt
    33661 atataaaaaa agattttagt agagcataaa aaatttctca atatatttta aaatatttct
    33721 ataaatatat tgagaaataa aaaaagagta ctcaaactca actagtcttt aaatgtggat
    33781 ttagggaata gttagtaaga atttcgatta aagcactgct aatctcttaa aaatgcaaaa
    33841 aatgattaaa ttaaatattt atactcattc agtataaata tttaaaaaat aatataaaag
    33901 atttatattt tagttttagg aggtttaatt ttgaataatg ataagaaagt tataaaagta
    33961 cataataaat cagataaacc tgataatatt gttatcaagg aaaatgaact catagaaaaa
    34021 tgtctacttt tggaaaataa acttcctgta tttatgaagg attattttat atatctaaaa
    34081 ggctctgttg cagtttctac acgacttgct tatttagaag atattaattt tttttgttca
    34141 tatttgattg aaaccaaaga attaacaaat gcagaatgta taaaagatat tacagaagat
    34201 gactttaata caataaaatc aagagatata aatttattct tgggtgatta ttgtagtaga
    34261 tattataaaa atacagaaaa aaacacattg atttttgaaa ataacaatag agctttggca
    34321 aggaaaaaat catccatctc tactctattt aaattcttat acaggaactc tcagatagat
    34381 aataatataa cggatggatt caatccaata aagcttccta aacctcagcc agatgccatt
    34441 aagcgtcttg agatagacga agttgctaag atgcttgaat cagtagaaac aggtgaaggg
    34501 cttacagaaa aagaaaaggt atattggaga aaaactaagt tacgtgataa agctatacta
    34561 gcattatttg ttacatatgg acttagatta aatgaactta gagaactcaa tatctcctct
    34621 tttaattttt caagaggtga atttaaaata tatagaaaac gcggaaaaga agttttaatg
    34681 cctataaacc atacttgtga acatgtaata aaagattatt tgcaaaatga aagaacaaga
    34741 gatgaactat taaatgatga agaaaaagat gctttatttt tatctcttca aaataagaga
    34801 attacagcaa aggctataag aacactagta aaaaaatata catctattcc acttgatact
    34861 acgagagaaa atggatatag tcctcataaa ttaagggcaa cagctgcgac ttctctaatc
    34921 caaacaggat tttctatata tgatgttcaa aacttacttg accatgataa tgtcacaact
    34981 actcaacttt atgcagctca caagaaaaat gtaaaacgtg atatagtcaa gaattttgaa
    35041 tggattgagg atgattaaaa atcaggaaca aatgtttgca gatataaatg gattatgtta
    35101 taattaaaaa tataaagtgt atggaaataa aaacaaaata tgtttggaaa tgagtgatgt
    35161 tatgtattta gatctaactg aaaagcaagt attgatattg gaatttataa agtctcaaat
    35221 aatattaaaa ggttatccac ctgctgtaag agaaatatgt actgctgtag ggttaagatc
    35281 tacttcaact gtgcactctc atctaaataa acttgaaaaa ctgggataca taagaaaaga
    35341 ccctactaaa ccaagagcta ttgaagtttt agaacgtagt aaagtgaatg atgtttctgg
    35401 agctaatcaa gaaataatag aacttccttt agtaggtcaa ataacagctg gagaacccat
    35461 tttagctcaa caaaacatag aggaatacat tccattccct gccagtttag taaagggcag
    35521 taacaatttt gtattaaaag taaaaggcga aagtatgatt aatgcaggaa ttttagatga
    35581 agattacgtt gtagtagaca aaaaaaatac agcctttaat tctcagatag tagttgcact
    35641 tataaatggt gaatctgcta cagttaagag attttttaaa gaaggaaatt taataagact
    35701 tcagccggag aatgatttta tggaaccaat tatgctcaat gactcagaag ttgaaattgt
    35761 aggcattgta actggtgtat ttagagttat taaatagcta ttaaaaaata cataaaaaaa
    35821 taagctattc gtatagataa aatgtgttat ctattgatta tagcttattt ttttatgtag
    35881 ctcagaagct ttatatataa atggttgatt ttttattatt ttacataaca tatatcacat
    35941 gggtcatttt tcttactttc tgcaattgtg cctgataata tttttttact tcttgaaagc
    36001 ccactacaat tttttgtaga gtgataactc ttcccttttg gagtaaaata cactatttcc
    36061 gctgaattag atgtattttg tttattttta aaagaatcat ttttacttgt attattgttt
    36121 gattttccag agtttccatc ttctctaacg cctttattgt aacttccatc tttacaactt
    36181 gtaaatactc catcacctgt tgattcaaat attatatctg aacactcatc acttctatgt
    36241 acttttactc ccattttttt taatttattc attgtctcct gatgtggatg accatatgag
    36301 ttacccttgc ctactaaaag aaccgcatat tcagggctta ctttatccaa aaactcttga
    36361 gatgaactac tacgagaacc atgatgagct acttttaaaa ggtctacttt atcaagttta
    36421 gatagtattt ctttttcagt accttcttca gcatcacctg taaataaaac tttatcattt
    36481 ccattagtat atacaaggac tagagattgt tcattagtag tatctcctcc atttgtattt
    36541 agaacctcaa aataagaatc ttctaaatag aatttattat tttcaagtgg tacacttgga
    36601 gataaatcct tacttgctaa agcgtttata aagtctctat aactctttgt ttctgctgaa
    36661 ccattcgata caaaaacatt ttctatattg aaatttttga caacagaatc aagtccacct
    36721 aaatggtctg catgagaatg ggtagctata agatatttaa tatcttttac accttgatta
    36781 tttagataat ctaccattaa tttctcatca tcattatctc ccccatctat caatgtaaaa
    36841 gttttaccat cttgaatcaa tattgcatca ctatttccgg tattaataaa atgaattttt
    36901 acattcttat cacttgatat actactagac aaagagtcat tattatttga ttgattattt
    36961 ccattgtctg atgtagcaca accagttaaa taaattgata tagatactat aaataaaagt
    37021 atcaaagctt taattttaga atttttttta ttttttatat ttctttctat cacaaactac
    37081 gcctccatat tcataatact ctagtaatta taacatgata tgcgttttta tgatattttt
    37141 ttacataata ttttaaataa tagattatgg tgcaataaca tagaaaattg acactgtata
    37201 atttttatat tatataaaat gaaatatttt atatcagtag tatccagtta tgcattttaa
    37261 acgaccattt acgtttaata tattgcaaac tgattctaaa aattataata tgttaagtat
    37321 caacaaaaat aaataaactt tttccttaaa gaaggcttaa agctcacacc aactgccttc
    37381 ttttttttta taaaaattta attatttatt ttgattaata tttatttcat attttagttc
    37441 tactcttcta gatgcatctc tattcacgct attatcacca tttttaatga gttcagcttt
    37501 tgatcttcct attgcaataa tatgcttagt cactttatct ttgtatttat agtcacctat
    37561 ttcatctccc actataaatt tagcaacact atatgctctt ctttgagata aatctaagtt
    37621 gtatatataa cttcccacat catctgtatg accttctata actattttgg atatatagtc
    37681 tccataatct ttataaatag tctccatgta ctttggaacg aacattttta acatctcttt
    37741 agcttctggt tttaaaacat cactatctat atcaaacatg gttctttcac caaaagttac
    37801 ttgaccatta gatttatcta ctttcacagg tattccactt tttttaaaag tttcctcaac
    37861 agaagtattg acattcttag ctaaatcttg ttcaacagaa cctgaaatac tttctgagct
    37921 aaataagata agaatcacaa ccatcaatac agtagataat aaatctgtaa atgttggcca
    37981 aaagctactc ttttcaaatt ctctgttaac agttcttcta tatttattat tcatttaact
    38041 catcctcacc tatgaccttt atttctttat cctctatctc ataaatttct tcactaaaag
    38101 gattaaatac attattaaca tcatcattaa ataattcttc actatcaaga ggaacttcat
    38161 cattcataaa agagttatta aacataacat ctaacttatc ttgaatatct aaagaatttt
    38221 caaacatcat atccagttta acttgtaggt cattagaaaa gttttcaata atatttgaaa
    38281 gattttttat tgagtcatct aaggtaaact tttcatctaa aaggattttt tggttttgag
    38341 atattttact ttcatgagtc attaatttag ttaataaatc ttgagcactt ctttcatata
    38401 gggcaaatcc tgcatttata ttttttatat aatctctaat tttgaactga ctttcagtgg
    38461 cattgtatat attttttgaa gattctttta tatcaaataa aatctcttta tttatgttat
    38521 cttgattatc atatttatca aaaagcttta caaactttat atttaaaata ttaaattgct
    38581 tatctaattt tcttacactc tggtcaaatg tcttaataga actctgagaa ccttctaaaa
    38641 ggctttttaa agcttctata ccactaataa attcatcatt aaagtcattt aagtctttag
    38701 atattttatc gaatttttca atagcttcta tagacttgct tatttgtttt attgtatttt
    38761 ttacctcttc tacttttaaa tctattcttt ctgacttaaa atgagttatt tcagatgtta
    38821 gtaaattttc agactttaac attatttgta tcaaaacatg ttcacaatct tttatcttag
    38881 taaagtaacc tataattaga gaaaatacaa ctccaaatat actagttgta aaagcagttt
    38941 gcatactact aatagttgaa ggaagtgaat ttataatgtc tttagtatca acacataaaa
    39001 gcattgtaga aagaccaaca aacgtaccta aaactccaag taatatactt attgaagatg
    39061 aattttttat acttctaatc ttttctaaca atggtaaatt attatgcttt aaatcagata
    39121 caacttcctc tataaatgaa gttatattta cgtctgcata aggattttct ttactatatg
    39181 tatagtagtc atcccttatc tttttataaa actcacttgt cttatagtct ctattgattt
    39241 cgtctaattt tgaacttaca aatgaatata ctctgttatt ttctattatt ttaaaaacag
    39301 aatacacagt tattgcaagt aaaaacaata tagttattgg attagtaaat attgactcta
    39361 ttaaattcgt tattatgtta ctcaaattta tcccctacct tataatttat ttatctataa
    39421 tttatacgta caagttacta agaaaatacg taaataaaca aaatatttat attatattta
    39481 tgaaaaatgg tacaaatagg tcatattgtt aataacataa tatatacgag ctaaaagtta
    39541 tagggggtgg cataatgaat gacaacatag tagaagttaa aaatataaat atgaacttct
    39601 acactaagga aggcgagctt gaagtattaa aagatgtcaa ttttaacttg aaagaaggag
    39661 aaatattaac tttacttggt ccttctggaa gtggtaaatc tacaatatta aatattctta
    39721 ctaatctttt aaaaccaact agtggtgata taaagattac tggaaatatt ggatatatgt
    39781 ttcaaaaaga taatttgctt gaatggagaa atataatgga taatataact attggattgg
    39841 aaattcaggg caaaaaagat aaaaaatctt tagatagagt tgaaagcctt ttaaagacct
    39901 atggattatg ggattttaga agtatgtatc caaaagaatt atctggaggt atgagacaaa
    39961 gagttgcgct tattagaaca ctttctgtaa atccagatat tctactttta gatgaacctt
    40021 tttcagctct tgattatcag actagactac tagtttgtga tgatgtttat agtattatta
    40081 agaatgagaa taagtctact attttggtta cacatgatat tggtgatgct atatctataa
    40141 aagtgcaact ttagattatc aacaataatc acctttaaaa ttttctaaac tcatcataaa
    40201 gttttcaaag actatgcttt gtaataaatt aataaactca ttatataata ttacaaattt
    40261 atataatgag tttgatttct ctttaagata caaaaaaata tcatttatac ttgcttgtta
    40321 cttctttact atctcttcca aaatattttc ttataaaata agaaggtgta caactccagg
    40381 catgacaata gctgtttatt atccttgaac cataaggaga tacatattta tcatttggat
    40441 tatatagctc ccagaaacaa tcagctccat ccttaaccat ttcaccccaa taacctctta
    40501 tacattcaag agctttttct tttctattat ttaatattaa acattcaact aaatgatgat
    40561 atgcatatgg agtaactata tccatatcag gtggattatc taataagtta tctaatatct
    40621 ttgtatttgt ttctttatca aatacatcag caagaacaca ccatatttga gatatccaag
    40681 aaacttgctt ttcactacca cttacaaaga atccttgttt ttcatcccat agatgatttt
    40741 tagcaccttg aaccactcta tctatttcat actcaagctc ttcttttttt tcattatcgc
    40801 ctaatattat agctaattta tgagcttgtt ttaaagcata tattaatact ccctgtgcac
    40861 ctgcttgttt atttaaccct tctttccaat ctaagaaaca ccaccaatca tcactatcat
    40921 aaacaacacc attaattcca actctttcta aagctaactt agcttgaatg tatgcaactt
    40981 tccataattc atttaaaatt tccatatcct tagttgcttc atagtagtca tgtaaacatg
    41041 atatgaagaa taaggaatag tcaaataaag cagtatcatc cactaataat ttaggttcta
    41101 taaataaaca tgctcctact tggcctctat tttgagttaa tcctgcgaaa agatataaac
    41161 atctttttac taaatcatta tttttaaaag tttcataatt cgttatagct tgtagtctta
    41221 aatctcctat ccaaaggcgt ctatctcgtt ttggcccatc ttcaaaaaca gtctgcatgc
    41281 aatcttgcat agttttcaaa gctattttat cgattttaat taagtcatca tcaatatttg
    41341 atagataatt tatctctgac atatctactg acgatacaaa attacaagat acatcttcta
    41401 ctattacttt atatttagga gatgtatcta atacctttat ttccaaatat ctaaatgcat
    41461 atcttctttt catattaatt ttatcaggta aaatatctat atgcatagtc tcttcttgaa
    41521 tccaactact acttatgctt cctttgtaag ttgaactatc ctctcctatt tcacataatg
    41581 tttctcccaa tttaattctt aaatgagtag gtgcatctgg tgggctacct attggtttta
    41641 ttttaaaatt tacatatccc acttggtgat ttccaaagtc aatacaaatg ctctctcctt
    41701 tacctaaact tatattagat atttcatcta ttgattttgt atctataacc tcccaatcat
    41761 ttttacccat tttaatatta acaatttgct taggtttaat tagtttttct ataaggattg
    41821 gctttagttt ttctgcttta tctatgaaat cttgattaat ttccattctg aagtctttta
    41881 tactatttgt aatattagac atgtttattc tccttgattg tgtcattatt atttattcct
    41941 cacttatttt tctagcatct aaatctgctt tgattttagg atattgctta tctaagtcat
    42001 aaaatttcat agttatcatt gagcatatta aaagtgccat tggaatataa acatacatta
    42061 ttttaatagc tactaatgca cttgctggtt gaacttccgc tttagctatg tatccacccc
    42121 aagctaggag tgctccacct aaaccgcctg atatagcttg tgcacatttt cctaaaaatc
    42181 catttactga gtttaatgtt ccagttgtat ttatcccagt tttccaaatc ccataatcaa
    42241 cagggtcagc ctgcattgag aagtatatac tttcttttat tccatatcca aaggcagaaa
    42301 ctatagaacc tgctataatt aaacttatat ttaaattagc tattagtatt attcctagac
    42361 ctataaattg tacagctgct gaagttatgt atagattacg ttttcctaat cttttagcta
    42421 gaattgggac taagaagttt gcaagtattg gtattattga cataattgta gccactgttg
    42481 ttgccaacga cttacttcca acattgtatg aaaagtaata tattaatgca ctagattgta
    42541 taaagaatcc agtccacatg aataatatat ttaaggcaaa tagtttccaa ggtttatttt
    42601 cttttaatga ttttatagtt tgaaataatg ttacattttc agattttatt tcaactcgtt
    42661 ctcttacatt tgagaaagta aataagaata ttgcacatcc tataaatgca aatagcatca
    42721 taactacttt aaaccctaga gcttcatcag tgcctccaaa ctttcccact aaagttaaag
    42781 cactataact tactattgtt gaacctaaaa atccaaagaa ttttcttgat gttgataatg
    42841 ctgttctctc attagtatca tctgttaaac ttggtaatat tgaagccatt ggaatattta
    42901 ctatagtata agttaaagat aatccaatat atgtaacata agcataaatt aactttccac
    42961 ttaatgatat atctggaacg ctaaatgcta gtactgcgaa taatgcaaat ggtactgcac
    43021 caaataggaa atacggtctt gatttacccc aacgagtatt tgttttatct atacaataac
    43081 caaccaataa atctgtaatt ccatcaatta cccttgttac aacaaacata aaacttattg
    43141 ctacagcact taatcccata atatctgtgt aaaaatataa aagatataac gatatcattt
    43201 gccaaattag gttacaagct aggtcagatg acccatagcc tattctttga cgccaaattc
    43261 ctggtacatt cttttcatca acaactttta aagtagtcat ttataatccc cccttatttt
    43321 gttgttttta ttatttctaa tgacttaaca tcattatatc ctttagaaaa tgcttgaact
    43381 attgtcgttt ttaaccaatt taattgattt attttcatat ttatgatatt tttattttat
    43441 atttaaacaa tcttagagat gaaggagatt ttatgagttt aaataaatta tttcaaaatc
    43501 tttatattta taatgaatct gagttatttt ataaaaatta ttattttgca aaacaaaatc
    43561 cagaatccct aaaactattt attgataact tagatgagga ttacatagtt aataataatc
    43621 taattattcc atatatttat aatgaaaaga ttcctcaact tatgaaagat gaaacttatt
    43681 ttgagtgtaa cagcaaggat aacgttatac ttataaaaca taatcgctac acacctcaat
    43741 ttgtgcatga gcatatattc tttgaaatga tttatgtctt atcaggtaag tgctatcagg
    43801 taattaatca tcataatgta catatgaaag aaggagatat ttgtatagtt tcacctggag
    43861 taaaacattc tataggagta tttgatgata gtattattat caatgtatta atacgtagaa
    43921 gtacttttga ggatgctttc tttgattttt taagaaacaa caacttgctt tcatccttct
    43981 ttttaaatag tatttataaa gacaaacaga atgattacat actttttcat acaaataatg
    44041 ataaatcaat agaaatctct attttagaga tgctttcaga atataatgaa caagatagat
    44101 attataataa tatacttaat catttaataa tggtgttttt tgcaaaatta cttagagact
    44161 atgaagatag tatagaagtt cctaatgtga cgaaaaaagg tactttaaat cacacaaata
    44221 ttcttactta tatccaagat aattatgttg atataacctt agaagaactt gctaataaat
    44281 ttcacttttc tataccttat tgttctaaaa ttattaaaga aattgcaggt tgtaatttta
    44341 ctcaattatt acaaagaatt agacttgaaa gagctgaaag tttgttaatg aatacaaata
    44401 ttcctattgc tgatattagc aacaatgttg gttatccaaa tgtagagcat tttattagat
    44461 tatttaacaa aaaatataat atgtctccca gtaaatatag aaaaaatact actaaagtat
    44521 aacattgttc ataattaaag gctatgaatt aaaattcata gcctttttaa atatgtgtgt
    44581 ttaataagag tttttatttt aaactattta tttgattatt attaacttaa tttggttctt
    44641 gttaattttg agatgcccaa tctgctggat atgttacata tataaatcta gcataatcag
    44701 gtacagaaaa ttgtatctta cttccttttg gtataaatat taactcacct gaagaagcag
    44761 aaacctttct tccatctatt attatatcta aagttccatc tattacataa tctatttcat
    44821 catagtttag agtccaatca aaagttgttt ctttcatttc catcatacca caacctaatc
    44881 ttgggctttc ctccaatgta aataaatcct ttgtgtaaac tacatcactt ggatttcctg
    44941 tatctaatct atcagattca ctaactttta cagttggtaa ctttatagaa gtaattccac
    45001 taatatcttt atttctaaca aaatctactg tgtccttagt tccacttatt ttttcttcta
    45061 ttatttgtct tactaatgtt tcaattaaat ttttatctat atttgatata tccatcttat
    45121 acattctcct tttttagtgt cttatttgcg ataaccatag ccaataatac tgctgttata
    45181 ccgccaacta atttagcaac tatcattggt gttatcatat tagaattaaa tcctgctgta
    45241 aatcctaagt ggtctccaaa tacgaatgcc gctgatacag agaatgctac atttattatc
    45301 tttcctctat tgtccatatc tttcatcata ccaaacattg gtatacagtt agctaaactt
    45361 gcaacaagcc ctgctgctga cacatcattc attccaagaa cttttcctaa agccattaat
    45421 ggtttattaa atacctttgt tataacaaac actagtggga atgctcctgc caaaactatt
    45481 gctatatctc caactatttc tataccttca cttattggct tcattccagg tattacaact
    45541 atccctgtta aagcttcaac tatagcagca gcaaatccta aagttataac tattactaca
    45601 ccctttccaa agtatgtgaa cccttttatc atagcatttt caaatttcaa taatccaagt
    45661 gctataacta ctgcaaatat tattattggt actaagtttc taagaaccat cattatctca
    45721 aatcctgcaa ctaatccacc aacgaatgct ccaataggta ttgttattat acctgcaagt
    45781 attcctgttg ctaagaattt ttggtcttct ttttcaatta ttccaagtgc aactggtatt
    45841 ataaatacta ttgtaggtcc catcatagca cctactatta atccaccaaa taacccagca
    45901 ttagggtctt ttgcaagttc aagtgcaagt ggagcaccac ccatatcatt agctaataaa
    45961 cttccagcaa acatagctgg gtcagctcca agtatattaa atactggcac tacgattggt
    46021 cttaatacat cagcaagaac tggtgctaaa catataacac caaccattgc aactgcaagt
    46081 gaacccattg ccattatacc ttcttcaaat tgctctccaa gaccaaattt gttacctata
    46141 catttatcta tagctccaag caccatgaat attaccatta catagattat aatttcgttt
    46201 atactcataa ttccccccat tattttatta gactttttat tatttgactg tctactgatg
    46261 gtattacagt cttatatatt acttcttttt ctcttactac atcttcactt tcttttaatg
    46321 catattctaa actacttaaa tctcctgcta ctatatatac taattttcca cctatagcaa
    46381 aagataattg aagttttact aatactaatt cgcctgattt taaagtccta tcaagcattt
    46441 ttattccagc acatacctta ccactttcaa caacacctat agagactata ttatctaact
    46501 tttgatactt atttttaaat ccatctaata tttcttgtga aattgcattt attacaaagt
    46561 tatctacaat atatccttct ccaagtttta ttccataatc tacacattca tttacataag
    46621 aagtttctcc acctactact attagaaatt tacctggaca aatacttttt aagtacaata
    46681 catcaactga agatttcttt atcatttcat tggatacttc tataccttta gcaatacttt
    46741 taaattctat agctcctata cttttattca tcaaacacct caaacgaatc gactactcca
    46801 actatagttg catctactgg aacatgagct ttttcaatcg actctctagc tgaggaacct
    46861 ctggatataa gaaccaagtc tccatttcct gctcctgcat tatcggctgc aaccatgaag
    46921 ccttccttgt atgtgccttt agtttttaaa atttttacaa ctaaaagttt ttggccacat
    46981 aatttttcat cttttcttgt ggcccaaaca ttacctacaa cttttcctat atccataaaa
    47041 ttacctcctt atactttctt tagtttgaga tggtgtattc tcataaaatc tacagctaat
    47101 ggtgttatta tacttttttt attaactagt atgtttttaa ctccatttat agttggtttt
    47161 cttaagtcag cttccgatat aagttttcta tttcttaaat ctaaactaaa ttcattatct
    47221 atttttacac tttctacact ggttaaattt aaaacatctt tatctaaagc ttttgtatct
    47281 tcttttttag aacctatagt actttctagt tctcttttta tgtttaactt atttttagtt
    47341 atagcattta agtcttttat aatttctaca ccaaatttac atatttcatc ttcaaatgat
    47401 ataaatttct tatataatgc ttttggtgca gtatccttat attttttgta ttcaattcct
    47461 tcatctaaaa catacacttt tttgccactc ataatcattt ttagtataaa tctttcttca
    47521 tcacttgttg aatttccaag tgctatattc gataatcccc tcatacaaag tcttgaaact
    47581 atcactattt cacattcctt tatactttta tcaaattcca ctatttcaaa ttcatctttt
    47641 aataagttaa atttattggt gtcttgttca aatacaatca cagctttagg tttattagat
    47701 atttttattt cattgctatt tactttttta tagatttctt ccattattaa atctacaaga
    47761 ttatcataat tcatattaaa tcctccccag aggtaattta tatagtaggt taaatttata
    47821 ctttctttat tattttagca acagtccctt ttgaataacc acatgcattg gcttcatcat
    47881 aatctatatg catataagtt ttaaaactgt cactcactct tattactaca tcatcaaatg
    47941 ttaaaggtct ttgacccatc actttggctt caacaatttc tttatcacat acattgaaat
    48001 tttcagcgtc tttaggagtc atgtgtatat gtctcttagc aaccattaat cccttatcaa
    48061 gttcaacttc accatgttgt gttgatattt taatacttaa agtcccttct aagtctccac
    48121 tctgttttat aggagctttt agtccaagag ttaaaccatc tgttaaagat atttctacct
    48181 gagtttcatt tctacatggt ccaagtacaa ctacattttt tatacttcct ttaggaccag
    48241 ttattgtgac tctttcctta caagcatatt gaccgggttg agataggtct tttagttttg
    48301 taagagtata tcctttacca aataatttat ctacatcttc tctacttaag tgaacgtgtc
    48361 ttcctgatgc ttcaacttct ataaatgctt cttttttaac tctagttatt acttcttcca
    48421 ctatttcatt taatagattc tccattgtgt cctccaacta cttatactta ttagttctaa
    48481 ctttatatat cattatccaa aatactgacg ataatctatt aagtgccttt attaaatctt
    48541 ctctatgaac atttccatac tcacccttaa atgcttcata ggctgcaagt tcaacctctc
    48601 gtgtattact tcttatacta tttatagcta ctactacttc tcccatttca taacttggga
    48661 actcatgtcc tataccaaag tattttttag gatagtgaga ttgttctcta agttcttgag
    48721 gagttaatcc taaaagttta aagtcctcta gtttttcttc taaaacttca catctcatta
    48781 tatttcttac aaagtctaaa acttcttgca attccttaac gagctttggc atatctagtt
    48841 tctgacaaag tatctgtgcc tttagtattt ctgattcaag agaatctatt tttcctctca
    48901 atattattct tttatggtct ttaaaaacta aaagatttcc atttaattga gtcatgtgtt
    48961 ctggtttttc atccaattta gcaccaaata cagtagtata tttatatgtt ttttctttaa
    49021 ctacttcctt tacaacttct ttaggctcat tatttacatc tgaaagttta tcaacatatt
    49081 taagttcaat acttctctca gctaaataag attttgcaga aggagttatt atttgtcctt
    49141 tttctatata aaattcagtt atattatttt tgttttttcc aatttttctc tttatttcgt
    49201 tttctgttaa aacacccatt ttatcacatc tcctattaga tgtataaaaa agactacctc
    49261 aaaatacatt gagtttatta gtaagactca tttttgagat agcctctttt tcaatcaaaa
    49321 tttaaatttt aaaattattt ttatattact cagctgatac ttttggtaat atagcatcta
    49381 cttcaaagtg tggtcttggt ataacatgaa ctgatattag ttctccaact ctctcagcag
    49441 ctgcagctcc tgcatctgta gcagctttaa cagccccaac atctcctctt accattacag
    49501 taactaatcc tccacctact tgctcttttc ctactaattg tacatttgct gctttaacca
    49561 ttgcatctgc tgcttctatt gctcctacta atcctttagt ttctatcatt cctaatgcgt
    49621 ttgcacttgc cataatctta tcctccaaaa atttaatttt atagtttata tttttagaaa
    49681 tttatagcta tagctataag tttcattgtt tattttagtt atcctagttc tttgactagt
    49741 ttttgaacaa gaagatttat taattcttct ttatcaattg agtcaaacat tgatttttca
    49801 acttgagatt tatctcttcc aagttcttct atttctttga ttccataagc tactctttta
    49861 acatttatta aatcaagtgg tcctatattg tttgaagttg aacttccacc tattgcacca
    49921 caaccaagtg ttagtgctgg tacaagattt gttgatgcac ctattcctcc aagtgctgaa
    49981 ggagtattca caagtattct tgatactggc atgttaagag caaatctttt taccaattct
    50041 tcatcatttg catgcatgga gaatgtgtga ccggcacctt catttaataa tatttctcta
    50101 catctgttca taatagcttc tacattttct tcaacataga acgctagtat tggtgtaagt
    50161 ttttctcttg aataggctac attgtggcca accttatttt ctcttgcaac tagaactttt
    50221 gtatttttag gcacatttgt taagtctgct aaatttgcta ttacctcaac actcttacca
    50281 actatttgtg gattcatagt accgttagct ctcaatataa attttgctaa ttttttactt
    50341 tcttcttcat ttaaaacata tgcaccttga tttttaagtt cttttaaaac agtctcttcc
    50401 atagattttt ctattactat agattgttct gatgcacata taacaccatt atcaaaagtt
    50461 ttagaatcta ttattctttt gattgctaac ttaacatttg ctgatttatc tataaatgca
    50521 ggtccatttc caggtccaac tcctatagct ggtgttcctg aagagtaagc agctctaacc
    50581 attgcttctc ctccagttgc tagtattaag tttgtatctt tatgtttcat cagttcatta
    50641 gttccaacta tacttggatt agttatacat aatattgaat cttctggagc tccagctttt
    50701 atagctgcat cacttattat attaactgtt tcaagtatgc aattttttgc atttggatgt
    50761 ggagagaaaa ctatagtatt tcctgctttt agagatatta tagttttgta tattactgtt
    50821 gatgttgggt ttgttgaagg tataagacct gccacgactc caactggtgt agctatatct
    50881 attgtatttg cttctttatc ttcactaatt attccaatag tttttgtgtt ctttaaagtt
    50941 tcataaactc ccatagatgc aaaagtattt ttaattactt tatcttccca ttttccaaat
    51001 ccagtttctt catttgccat tttagctaat ttagtagcat tattaaatgc tgcatctgca
    51061 atagctttta ctattttatc tatttgctct tgactcattt tagaaagttt atcttgagcc
    51121 actttagatc ttcttacaag agttctgact tcttgtatag atactaaatc tttatctaag
    51181 agtgtcataa ctatctatcc cttctgctaa gctttttaac taagtttatt aattcttctt
    51241 ttttaagaac atttaaatct ttataagtta ttgaagaatc taatttctta gccatagctt
    51301 taagtttttt cacgctgttc tttgataggt catctctatc aaaatcgaaa ttttctattt
    51361 taacctgttc caatactttt ttatcaatgt tagtattttc tatttttccc ttattagctt
    51421 ctgttaaaat atcatcaact gtttcaattt ctttgttgat tttatctata tcactatata
    51481 atttttcaat atttccacta ttctcagtga catctaaaat tacatcttct ttttctgata
    51541 aatttttcga tacattatca gttgtttcca atgtaacctc aaagtttttc tctttatctt
    51601 ctgaaacttc acagacttta ggttctttag ttttgaataa agcatctgtt acttcatcat
    51661 ggattcttgg tataatatgt gatgttctta atgtaccaac tctggaagct gctatttcac
    51721 ctgcttctaa agcagatttt acagcaccaa catccccaca tattgtcaaa gtaacaatac
    51781 ctgccccaac cttatcaatt cttacaatat taacatttgc agattttaaa caagcatcag
    51841 aagcttctat tgcagcaaca tatcctatta cttctattaa gcctatagca tccatctaac
    51901 tatccttaaa atcctgttgg attgtctgca actgatttta ctgcttctgc aaatgcatca
    51961 catgctgctt tacaagccga ttgagaacct gtaagtaatg ctccaccaaa gtttgtttca
    52021 gttggaggtg caaataattc acacatttta acatctgctg ctttcattgc tgcatctagt
    52081 gcatacattg cttcaagtgg tggtgctact aagtaagcta atgcttcacc ttctcttatt
    52141 cctgctacct ctgaaaggta tgtacctgtt cttgatacgc agtgagcata atatgctatt
    52201 gaatcatcat catttgcact tataaaagtt gctccatatt ctagaaaatc aactactgca
    52261 tttagaccac ttcttacttc tgctgggctt ggtcctgcta ttattcctaa tacttcacca
    52321 gcaagttttg ttgaagcatt tgcagcacct gcatacattg attttgcata tacaacatca
    52381 acttctgaag ctttagttgc ttcatcaaga gctgtgtacg taacatcatc acaatctgca
    52441 gttattaaac ctaaactttt gtgatgtgat tttagttcta gtttttgtgc catttctgga
    52501 cttatattcg aaattatttt aattcctagt atattaggac gaattacatc gtttttcata
    52561 ttctacactc cctcgacttt atttaaggtc tattcctgaa gcttttttat ctaacatagt
    52621 ttttataagt tcagctatat gagcacctgc ttcaactgca gttgttccac ctttgtgaat
    52681 gtttgatata actgttcttt ttgcctctgc cattcctata tatggcttat aagctatata
    52741 tgctgacatt gattcagcag taactagtcc tggtctttct ccaaccaaca tgcatataac
    52801 ttcacagcct aattcttcac ctaagtggtc catagcacca accctacaat gtttaacaaa
    52861 taaaatttca cttgaatcta taccatacat ttttagacct tgttttatag ctggcacaca
    52921 gtcttttaaa tttgcctcta tcgcagctga actaagtcca tctccaacta atattaaaac
    52981 cttttgattt gtaccaaatt tagattttat attatttata gtttctggag aaaattttct
    53041 tcccaaatct ggtcttgtta agtattcatc tttatcttta cataaagttt ctactgcaaa
    53101 catattattt tcttttataa attcttcatc tacataagag aatacagcat cttgtgcagc
    53161 agcatggtct gctctaaatc ttaaaactgt ttctgtttta tatctagtgc cggttcttcc
    53221 tatacctaat cttgcaggtg tttttgcttt catatctaaa taagcttctg catccttagg
    53281 attttttact agtaattgct ttcttatatc aacttctgtt atatcatcta tacaagcatt
    53341 gttgtctact acaacttcag ttgctttttt aacagtttca gatacaacat tagtatctaa
    53401 ttctccaacc atttgtccaa ctaattgttc tactaatgct tttaaatcct tctcattcat
    53461 attaaatacc tcctatccta ataatacaga cgcgtctccc gccaactctg ttaacttacc
    53521 atctttagag aatcccatct tttccatcca agcatcaaat tcttttattg ctgttagccc
    53581 aaacatttct ctaagtgatg ctgtttcatg gtatcctgtt gtttgatagt taagcataac
    53641 atcatctcca tgaggtattc ccataaaata cgtacatcct gctgttgtta atagtatagc
    53701 taagttttcc atatcatttt ggtcagcctt catatggtta gtgtaacaaa catcaacacc
    53761 cattggtaag ccatgtagtt tacccataaa gtggtcttca agtcctgctc ttacaacttc
    53821 tctagcatca tataagtact caggtcctat aaacccaaca actgtgttca ctaagaatgg
    53881 ttcgtatttt ttagcaaatc cataacatct agcttccata gtaacttggt ctattccatg
    53941 atgtgcttct gaagataatt ctgaaccttg tccagtttca aaatacataa cattaggtcc
    54001 tgtagctgta ccatacttca aagctaactg tctagcttct tctatagtat cgccattaaa
    54061 tccaaatgct tcatttcctt tttcagaacc tgctatagat tggaatatca aatctgcagg
    54121 agcaccattt cttattgctt ccatttgtgt agtaacgtgt gccaatacac atgtttgagt
    54181 tggtatttga tatttttgtt ttacttcttc aaatcttttt aatactttag taacactttc
    54241 aacagagtca tcaactggat ttaatccaag tactgcatct cctataccaa aagttaaacc
    54301 ttcaagaagt gatgcaagta ttccatctgg gtcatctgtt ggatgattag gttgaagtct
    54361 tgctgataat gttccttgtt ctcctatagt tgtattacag tgggcagtaa cccttatctt
    54421 tttggctgca taaactaagt ccatatttga cattaatttt gcaactgctg ctatcatttc
    54481 agaagttaat cctcttgata ttcttcttat atcaagactt gtagtatttg aatctaatat
    54541 ccattctcta agttcggata ctgtccaatt ttttatttct tcataaactt tttcatttac
    54601 agcatcttgt attattcttg ttacttcatc ttcttcatat gctactgcag gattatttct
    54661 tatatcctcc aattttatat gtgataaaac aacttttgct gctattcttt cttcagctga
    54721 ttctgctgca agccctgcta atttatctcc tgatttttct tcattagctt tagccattac
    54781 atcatataag cctttaaact gataagtacg tccaaataat tttgtcttta aaatcattta
    54841 cttacctcct tctattaatt aaaaactaat gtttttatta ccacaggtaa aactttacct
    54901 tcaactatag gttttccaat gtcgatatag tctccatttt caaccttgac actgtctata
    54961 caaacaatct cttttttata atttaataat gcatatatag cttgacctaa tacttttgcc
    55021 atatcatttt caacaattac tataaaaggt aaatcatttt ctataatttg ttgcattcca
    55081 tctacaaatc ctttggcata ctcttgtata tctaaaaagg ttggattttt ctttcctatc
    55141 atggctatag caactttttg taaatcattt tctagtttaa accaattaag tttatttttt
    55201 atagcttcac tgatttgttt tgaagtacct gtttcttcat caattgataa ttttaaaact
    55261 ggcaagtttt taagtggaaa cttttcgcta gtgtaagtta ttgtacttcc acttacttca
    55321 gtagtatgag aaccagctcc aaccactgta gctcttattg tttccataga tttaactaca
    55381 tttacttttt taaggtattt acaatttttt attgcttgtc caagtaaaat tcccaagtca
    55441 ccatattcaa aataatcttt tatttcccca tcatagtaaa tataatcagc tactccacca
    55501 gagaatgaaa catttttaat attttcatct acacttattg atttattggt tattataaaa
    55561 tcaaataaag gtgtctgttt ttttaatcct atgctttcca ttaataggtc aaccattata
    55621 tctactattg gttttgtatt ttctatggta gctattgtac ctacttgaag atttaaatta
    55681 ttatctttta tgattttatc tattttaggt gatatataag ttattttttt acttatttta
    55741 tctatcttta taagccttcc acctatatcc aaacacccag tatcaattac ttcacccctt
    55801 ttaaatgaag ctatattact agttccccca cctatgtcta tatttacaat tgtagttgaa
    55861 tgttcttttg aataaatatg agctccagcc ccttttcctg atattatact ttctaaatca
    55921 ggtcctgctg ttgcaacaac aaagtcccca gcaaatccac tcaaagtatg aagcacatca
    55981 tttgcatttt cttttctagc tgtttcacca gttattatta ctgctcctgt atgtatatcc
    56041 actttagata tatttgcttt tttatattca ttttcaacta tgtcttttat ttgttgacca
    56101 tttatttcat tatttgaaat aagaggagtg aaataaactt tacttttata aataatttct
    56161 ttgtctacta tacttattct aggtactgaa aaatttgatt cagtattttc tactattaat
    56221 ttagaaaaaa ttaactgagt agtacttgtt ccaatgtcaa taccaacact taataactct
    56281 tctctcattt tctcacctcc taaaatttaa gtatgtctta aacaagcaaa gaggcttaaa
    56341 gttataataa atatctatta aattgaatat atacttataa tttttataaa tacattcaat
    56401 taacttaatc tattaataac cttaagcctc tttgctcaat taaaactact actttgtaga
    56461 tgagttatta tttaaatatt aatttgtgta ggtatagtaa ttaatatttc tgtaccattc
    56521 ttattacttt ttctctctat atttcctttt aacatttgtg ttatataact atctattata
    56581 tatgtcccta aactattttt atttatatta tcgctatcaa atccaactcc atcatctata
    56641 atagcaatat ttctatagtt attatcttta tttattaata tttgtatagt accatgttct
    56701 ctatctaaaa aggcatggtc ataacaattt tgtagtattt catttataac taaaagtatt
    56761 gctgttgctt tttctccact aattttaaaa tcttcaccag ttatacgtat atctatatct
    56821 ttactaaata aatagctacc ttgaatattt gacactaata aattgatacc ttctcgtata
    56881 gatatattat tatcaacttc tttagatagt aaataatgag tcgaaagtat tgcaaatacc
    56941 ctatttatta tattatccaa gcactgctta acttcatcat tttgagataa tctactttgt
    57001 ttttttatta tagatattac tgtatgaaga ttatttttta ctctatgatg agcttctctt
    57061 atagctactg tttttaaaac taattcttct tcttttcgct ttaaatcact tatatcttgt
    57121 actattttgc aaaccttaaa ttcatcatct ttgatgtata tggtttttat tttaaaaaac
    57181 atattatcta ttgaaacttc atttgtctca tcataatttt catccattaa tatatcttca
    57241 aatgtattgg tttctatatc taagtcatta taatgcatat cttgtatatc atatccaaac
    57301 ccaagatttt tatatatctc agttgcaata cgatttttaa tttttaaatt tccattttta
    57361 tcaaaaatta gtatagctga atttaaatta tcaactaaaa agtcattatt tcctataagt
    57421 tttataaact gatgtaaatt tttattttct tcgtcttcac tagcgttaaa ctcattcttg
    57481 acttcactac ttatatcttt ttcaactatt aatactgcta aaactttgtc atctagcgtt
    57541 attggctgaa tattttgctt gaccagttta tattcttgtg tcaaagcttt tatatctctt
    57601 actctttctc ctgtttttaa gcactttata actcctggtt cattttcaat taaagccttt
    57661 ttgcctataa cattttcaca gtacattgac ttttctttag gtctaccatg tgccacaaca
    57721 attgcttcat catcaatttt acttagtata tctataaaaa catcagcctc ataaaaagaa
    57781 gccattaaat ctaaggattt tgatacttct ataatttttt caatttcatt attatttaag
    57841 tatgtttgtg cagttgatat tttttttatc gtattagtca tcgtatccta ttataatcat
    57901 tttggcaatt tccatcattg tggttctttt atccatactt aattttctta ttctagtgta
    57961 tgcttcatcc tcagaaatct taaactctct tataagtata ccttttgctc tttctactaa
    58021 ttttctttca ttcaatttag aatttacttt ttctaaatct tttttcattt tttcaaattc
    58081 atttacctta tttaaacaca tttctatagt tggaataagt gatttttcac ttgctgtctt
    58141 taccaagtat ccataagcac ctatactttt agccttttct acgtattctt tatcttcaaa
    58201 tgaagttaat aaaagtactc caccagctag attttcttta catataattt ttcctgcttt
    58261 tataccatca agtattggca tctctatatc cattataact aaatcaggtt tgtgtttttt
    58321 acacatttca acagcttcaa atccatcact tgcttcgcct attatttcat agccatgact
    58381 ttctaatata tcttttgtat ccatccttaa cagtggttca tcatcaacca atagtatttt
    58441 tttccccatg ttcctacctc gcttaaagat taaatcttct taaaatagtt tatacatttt
    58501 tttattttat catactttaa taatattttt gaataaaaaa atattttccc cttaaacttc
    58561 atctatattt ttagattttt ctagatattt aaataatttt tcaagtccaa tattatcaac
    58621 tgtgtctatt ttaaagattt gacttgctcc tgctagtgat agtctttcat aagctatatt
    58681 gacttcattt tcagtagctt tatttatttt agttataatt cctattacgt ctttgcaaaa
    58741 catacttgca aaacctggtg ctatataatt ttcttcttgt gtggcatcat acactattgc
    58801 tataatttta gcatctgctg atgttgttat taaggcattg tacaggtttc tattttccat
    58861 atactcacca ggagtgtcta ttgcattatt ataaagttcc actgattgag tttttttata
    58921 gtttaattct aactggtcta atttttgaca taaagtagtt ttaccactcc ctgtctttcc
    58981 cataaatatt atattcttca ttgtattatt atgtccttgt tatttttgta gatgagaagt
    59041 ttaacatatt tcctaagact tccaaaacat catttaacgc tgattctaca gaacttacat
    59101 cacctgatat tactactgaa ccactaaatc tatctataaa acctaaagat acatttgaag
    59161 cctttgtagc aacatctgct gctattattg acgcttcact tggcgttatt gtaagtattc
    59221 ctattgcatc ttttttatca agtactaatc ctaacttctt atatatatct tcattagggt
    59281 tagctatgat atgtgctaat gtaacctgtt ttccaggaac atattcttga ataactcttt
    59341 gcttagattc ttcagtcaaa tttattccct actttcttta aatatagttt actaaaaaga
    59401 taattgctta aaataaaagg agctccataa gaataatatt aatattccta agaagctcca
    59461 ttgctttcta ataaagtatc acgcctttgt gatactttca ttatagtttt atgttttata
    59521 tttgtcaata tataatttat atttttaaaa acattttcct tatttgtata agattatgtt
    59581 tacatgtaat aaaatgtagt tatttaatat tatttaatat gtttattata tcatcttcag
    59641 ttggtacctt aggattagtt aatgtacaag tatcttttat agcaagcttt gctatttctc
    59701 tttcttcatt agttatatct tctaaactta atttgcactg tgtcaattta gttggcatat
    59761 tcatttcttt ttgtagtttc tttatttcat ttaccaaatt tctaacagat attcttatat
    59821 tagcattccc aagtcctact aattttgcta tttgtgcata ttttttagct gtatcagagt
    59881 aagttgtgtt agtatatcct actattgatg aattatattc tattacatga ggaagtaata
    59941 tagagtttgt tcttccatgt ggtatatgga attttccacc taatacatgt gcaatagaat
    60001 gatttattcc aagtgatgct tggttaaatg ccatacctgc taaacaagat gcattgtgca
    60061 ttttttctcg agcttctata tcatttccat ctttatatgc ttttattaag tatttaaata
    60121 ctaattttat ggctttttca gctagtgcat ctgaaaaatc attagaattt gtagatacat
    60181 aagcctctat agcatgtgtc aatacatcca ttcctgtatc tgctgttata aagttcggta
    60241 cagtttttac aagctctggg tctagtattg ccatgtctgg aagtaaatca tcacttacta
    60301 atggatactt acattttttt tgactatctg ttattacagc aaatgatgtt acttctgaac
    60361 ctgttccact tgttgttggt attgctataa actctacttc gttaattttt aatatttttt
    60421 tagaaaagtc cattatagat tttgctgcat ctattgcaga gccaccacct aatgctatta
    60481 ttacatctgg atttatagat ttaaatgctt ctatccctga tactactact tctattggtg
    60541 ggtctggtac tatttcttca aaaacttgat atgttgagtt taccaaatta gatgttattt
    60601 tatctattgt ttttgatttc accataaatg ggtctgttat tatcaataca tttttgtttt
    60661 ttagttcttg taaagctttc agtgaacctt ctccaaattt tattctagtt ttaatctcaa
    60721 agttattcaa atttctgtcc tccttaagtt aaatacgcaa aaaagctcca tagactttat
    60781 ctatggagct ccattgctct tttaacactt cattgtgcta tatttaaaaa tatactattt
    60841 gattaatata atgtcaagtc atagtgtaca aattcaataa tatataaaat caataatcaa
    60901 agcttatgta ttagctttac ttatatttaa ttctttttct aaagtactaa atgcttcatt
    60961 taaagcttct ttaggatatt ttttacttat tatatttaca aaccaactag ctttattcat
    61021 ttctatttcg ttaagtttag ctttatcttt agggaaaatt tctataacat tgatttccat
    61081 atacaatccc cctataaatt tttgttatag ctattagttc caaaaaatta tcttttaaaa
    61141 tatttaaatt ttaatttttt catactaact tatgatttta tattaataat ctatgaaatt
    61201 agagatttgt cctgatacaa ttaaatacca aaatttatta ttgatttaga acttgaatag
    61261 taaagaaata aaataaatag tcatatatta taaaaagtag aattatataa atttacctaa
    61321 ttaataaatt cattctaata agtttactta aaagattgta tataaataat tctaaatata
    61381 tgtcctaact tataggaggt tttaattttg aaagctgcaa tttattcaag aaaatcaaga
    61441 tttactggaa aaggcgactc tatagaaaat caaatacaat tatgtattga ttatgcaaag
    61501 tctattggta taaaagattt tttgatatat gaagatgagg ggtttagtgg tagtaatata
    61561 gatagaccac aatttaagaa aatgatgcag gatgccaagg ataagaaatt taattaccta
    61621 gtttgctata gattagatag aatatcgaga aatgtatctg acttctctac cttaatagaa
    61681 aaattaaatg aactaaacat atcatttata tctatcaaag aacaatttga tacctcaact
    61741 ccaatgggaa gagctatgat gtttatatca agtgtatttg ctcaactaga aagagaaaca
    61801 atagcagaac gtataaaaga taatatgtat gaattagctc gtagtggtag atggcttgga
    61861 ggcaatacac ctcatggatt taacagcgaa aaaatcacat tcttagatga aaacctaaaa
    61921 gaaagaagta tgtataagct tcaagtaaat gatgagcaaa tggaggttgt aaagttaata
    61981 tacaataaat atttagaact taggtcctta tctaagttat ataaatatat ctataataat
    62041 ggaataagag gtcctcgtgg tggtaaattt gaccctagtt ctttatctag aatacttaga
    62101 aatccagcat atgtaaaagc taatgatgaa attttagaat accttaaaaa taatggtatg
    62161 gatattgtag gtactccaga taaaaaatgt ggagtcctta cttatgctaa aaatacaact
    62221 aactcaatag cagcaattgc aaaacaccat ggagtcatag aacctgaaat atggcttgaa
    62281 gtacaaaatc aactagataa aaatcgtgat aaaaccccaa ggatagcaac tggaaaaaca
    62341 gctcttttat ctggttttat aaaatgtggt aagtgtggtt ctaatatgag aattacctat
    62401 aaaggtagaa aaactgataa agatttaaag tattattata tttgtggcac taagaaaacg
    62461 cttggcgctg ataattgtaa ctgcaaaaac cttaatggtc cattagttga aagtttaatt
    62521 atagataaaa tcaagaactg taaagaggaa tctatcataa gtacattcaa taataataac
    62581 attaagactg gtaatatttt taatgtttta aaatccgaga ctaatttact taaaaatcaa
    62641 attttagata aggaaaaact tatcaaaaat ttagttatag agcttgcaaa aaatactgat
    62701 agcattgctt ctgattatat agtttctcaa attgagaact taaatagtga gattaatatt
    62761 ttaaaagata agcttaatac tttggagtct agtaatgcca atattagtag tgatagtatt
    62821 aatcttgata ttattgttag taatttgaag agatttaatg atgaagttga taatgccagt
    62881 ttagatgata agagattgtt attatctact attattaata gtatagtttg ggatagcaat
    62941 ttaggaaatc ttaatattat ttatatgggt gctgaaatgg ataattctgt ttctttaagc
    63001 aattagttgc acttttttag ctatagtaga agcaataagc atatcagata aggttgcaat
    63061 actatctaaa agaccagcta ccataaaaaa tatttacgat attgaactta acatggatgg
    63121 aacaaaaacg cctcttaaaa cacgtaaagt tcctgatttt caaaagtatt tcgacatatt
    63181 atggaaggag ctagatacac atgaggctta ataaaagatc tgaaggatat ataaactatg
    63241 ttaaaggtgt aaaaagagaa aaaagaaatg tattattcta ccagctatta attttaattg
    63301 gttttattgt aatctgggaa cttttagctg acttaaatgt tataaataca tttctattta
    63361 gcaaaccaag tgatatttac aatttattta ttcaatatgc atctagtggt caattattta
    63421 aacatattgg aatatctgta tatgaaactg ttcttggtct tgttataggt actgtattag
    63481 gtattttggt agcaattgct ctttggtggt cagaaaaatt atcaaaaata ttagaccctt
    63541 ttttggtagt tttaaatgct cttcctaaga ctgctttagc tcctataatt atagtttggg
    63601 taggtgctgg aatagaagga atcgtcgtaa cagctgtaac tatttcggtt gttgttacta
    63661 tactttcagc ttataattat tttatgaata tagacgaaga aaaaatcaag atgttaaaga
    63721 gttttggagc tagtaaatct caaatattat ttaaactcat attacctgca aacacaggaa
    63781 atttaataaa tttaactaaa attaatattg gtatggcatg ggtaggtgta atagttggag
    63841 aatttttagt ttcaagatat ggaataggct atttgatagt atatggttct caagtgttta
    63901 aattagattt agtaatgatg ggagtatttg tattggctat ctgtgcctgg gcaatgtacg
    63961 ctgtagttaa tattatagaa aaaatataca attccagata agtaaaaatt ttatttagaa
    64021 tatttgatgt ctcagtgatt ataataaaaa atgatttctt taaagtaatc atttttttaa
    64081 tataaaaact tatattaaat ttgattatct tttcataagt tggtaaatgt cgatatatcc
    64141 tgtatctaca aggagtatcg gcattttctt tttggaagat actttgcata agttggcatt
    64201 taaccgtata agaatgccat caaaagtagt aaataagtag taaactctag tcataccact
    64261 aggcagataa ccgttccatt tcagctcttg cagaactaaa tgaaccatgt gcataatagc
    64321 caagcgtcat agtaatgttt ttatgcccca ttatatattg taatatatta ggactcattc
    64381 ctttgttcgc catattggta cagaacgtat gcctaaaggt atgtggcgta atctttggta
    64441 gcttgtcctc atgagtctta ttatacttct tgactaacgc tcttaacatg ctttgataat
    64501 tggttgctta ctttggtaac ccatcacgtt taaggaataa gaagttttga taaacatcaa
    64561 caatgattgg ttttgcttta gcgttcttac gccgttccac gatacgttca aatgattgat
    64621 aagctctttc tcagtcattg gaatttctcg cttaccctgt gtggtctttg gaacttctac
    64681 ataatagcca atcttggtat cactcaacaa ttgatggtct accataatgg ctttattttt
    64741 catatccaat tctaaagtca aaccgcataa ttcagaaatg agtagtccta tttctaagag
    64801 gataacaaca tcatcataat atttacaata agtattatcg ctctccataa aggataataa
    64861 gtccatttcc tgttctgttg ttagagcgat ttttggttct gttgtatctt ccaaaacatc
    64921 acttaactga aaatcaaatg gattttttct aatatagtcg tcttgaattg ccatgaaaaa
    64981 cgacgctttc agtgaacgct tataattgtc aatggattta taagcacacc catcttcact
    65041 cattcggata gcccagcttt tagcgtctga taatttaacc atatcaatgc ttttcgcacc
    65101 tagtggctta ttgttcagaa tattcataag ataatggcgt cctttaaatg ctattgcgtt
    65161 cgaattaaac tatatacatg gcaatcaatc atgtttctat tctttattat ggaattgtga
    65221 cagtattctt caatcttaaa accacatttt tccaatactt tacttgacct tctattgtag
    65281 gaatatacat tagcatgaat tttgttaagg ttgtattttt caaaggcaat tttgcatatt
    65341 tcttttactg catttgttgt tatcccttta ccccaatagt tcctagcaag ccagtaacct
    65401 aatacagcat ttttattaat agaggtatct tcttcaaatg ttaggctgat acttccaata
    65461 gccctttctt gataaaaaat agcatagcta agttgtttaa catctggagt tgccaaacaa
    65521 aaactaataa aatctctagc gttttgctcc gtataagggt tggggaagtt gtcacgaaga
    65581 ttctttgcaa tttctttatc attcgcaaac tcaactaaat cggtaatata agcataattc
    65641 cactctataa gttccatttt tcttctcctt ttatgaaaca gctacaagaa atctttctaa
    65701 gccaatacca cttatcaaac agcgttcttt tttatttcca agcatttgat aagtccagtc
    65761 aacaaatcca ccatcaacca tttcaacggt atcatttcct tgtttgaccg ataatttgaa
    65821 attgatacct ttataataat tattatcaac atcatcatta tgaattgata actctatatt
    65881 aggaaaaata agtttgatag tttcaaccat tctgtcgaaa aatccgtcat tatctttgta
    65941 tccgtttctt ttgcgtagaa taatagagaa acgaacattt tctatttcac ttaatatatc
    66001 tttataatag tttaagtgct tttctaacaa cattttttca caagtgtaag accctgtatc
    66061 tataccagat gaaaccatac aatataagcc aaaatgtgaa tataaacctt tacctacaaa
    66121 attttgtgct cttattgccc tagcagtagt agccatgtgg ataggtatgg tattattttc
    66181 ttcttttctt tttaatttat cagcaataat tattgccaac atatttgagg ggtctgcaag
    66241 tgtttctgtc cctcgtagag cacttactac gttatattga tctacggagc caaaaacgct
    66301 acaactacct aatggagcag atggagaaag cataatcgtc ttgatatcca tattgaatgc
    66361 ttttctcaat aattttgatt ccaaagtatg aatttcttct ggatctatac tgctagggat
    66421 agtaaatcta tttagttggt atgactttag aatatcagtt gcagttaaag tatttgcttg
    66481 tctatcaaac acttctaata gaagtgagtt caaatctgat tttgatagag ctgataactt
    66541 atcaactata ttcttatccc caattttatt tgaaattcta cttaaaatat cactcatagt
    66601 tatcctcctt actgttactt tcaaaataat tatagaatgg ttacaacaac taatctaggt
    66661 cgtttgacca aaaatgaaaa aacactgaat atttaccatt caatgttttt ttactttggg
    66721 tttatttgat taaatcttga aaatactgta atgcaaccct ttcagcttca acctttcgga
    66781 caataggact ttgtgataat tcctctctta actcaagata caggttgata gtagcctgca
    66841 atgcattaac acagcttcta tattcatggg tagcaaccgt cttttctaat gcttcaatat
    66901 attggggtgc atccaattcg atttttctaa ctccacgagg taatttacct aagagaagct
    66961 gtatcaatgg ggcaagtact gattgtctta aaaaagataa aaaatcaatt gtttcaaata
    67021 gttctctacg tccaatctta gtgcaagcgt aatgaaccca aatccaaaat ctatcttcaa
    67081 tccactgtaa gtctggagtt gggaagtatg cttctttttt agaataaatt tctgttatgc
    67141 agttgttttc ttcatagaga atagctgaat cttcaaccct atctagtaaa ccatctaagg
    67201 ttgtaaactt aaaatcaaca tgaagaacag gggaatcata gagggaaatt atcaatcgag
    67261 gttctccaac gtgttcgcca gtaaaagcag aaactaatgt tccaaactgt tccacaataa
    67321 gtaatctctc ttcgataact tcttcatagg atttatcatc tgctacaatc agaaaatcca
    67381 aatcagaata ttcatctaac gattttgtga tataagaacc gctaattgca agtcctatta
    67441 tcctactatc tcttttggaa aattctaata cgttttgtat catttcctgt tgtgttttag
    67501 tcataatata acacctctca ctttctttta ttagctatca atccaacata attatgcata
    67561 ggagtattat ataatgaaga aataaaaaaa aataggtcac ttgacccgaa ataaaaaagt
    67621 attgaataat tattatccaa tgcttttttg aaattatact tatgttgtag gaagattctt
    67681 taatacctct cgctgattaa ttacactaaa tcttttgttt tcataagcaa ttagatgttc
    67741 ttgtacacat tcatttatag cacgattaat gcttctaatt ggagcgttta cttcgctagc
    67801 aagctgtttt ttggtaagca gatttaattc acttctataa tagtgtaagg atatacactt
    67861 cagaagtcgt tctttaacac ttaaagatga taattcttca attaattcag catttacaac
    67921 taatttacta gatatttctc gaatcagaaa ttttgtagct tcaaaatctc cctttagcca
    67981 gtttaaaaaa tcatctctat gaagtttata tacattacaa tctgtgaaag cgacaatttc
    68041 aacgggtttt ttcccatcat taaattgttc catttctcca aaaaaactac cacttctata
    68101 aagatgaata gttgagatgt ttcctttcgc atttaagatg aatgcttctg caataccgtc
    68161 aatcataaga attacatatt cattttcttg ctcagcaaaa agaatagtct ctttcttttt
    68221 atacgttttt ttaacaagct tctttttaat atcttcattc ataaaatcaa ttaaatgcat
    68281 attcatccct ccttaccaaa tatttgatta aattatatca tctttcaatt agttaggtaa
    68341 gatagtaaaa atatatttac atagaaacta ttccattatt attttccagt tgtcattttt
    68401 ctgcaaagta agctcaaact gggaaagctg tgtaactttg gtttccttat catagaaaag
    68461 gaacaccttt tttcgtgttc catgttctat taaatattta gttttatttg atacaatact
    68521 gaggtgttta taccgtattt ttgcatttct aggacttatc aacgtatcaa ggctcattcg
    68581 gtagtagtaa attagtagta aaatggcttt gttagtcctg tgtatatgcg gataggtact
    68641 gtgttctagg ggataatcag atttttagta tgttttttaa attaaaataa agcgtaaatt
    68701 tttgattatc ttcttataaa cttaccatgt tgccagtatg accctatttt atgggtttta
    68761 ctggcttttt attgcctcca attatacata aatacagtta tttttttatt acctatgctt
    68821 aaatttctat aaaagaaaaa acagccaact ttttaatcaa aattaactgt gttttatatt
    68881 caatatttaa ttttcatttt tgtactccgt tttgtgctcc gtttgttaga tagcagagac
    68941 ctaatgagtg ctcacagttt agcccaaaat ctgaagtgag ccaactattt gctgtgatta
    69001 aatagtttac cttgaaactt aaatttatct tctaaaatta tcttttaaac tgacgatagc
    69061 aaaaatatat gtattctttc attgatattt ctctactatg taaaattcct atgataacaa
    69121 atattatttc tgcaacagta agatagcagt cttttaagtc gaaaataaaa aagcttggta
    69181 tctgcaaaaa atctaaactt cctccccaaa ataatttatc tatcaagcta catattgtac
    69241 cagcaagtcc acaagacata attactttta ctgaatagct cgtttgttca ttttttgatt
    69301 tgtaaaaagc atatccagat ataataacta ggataactaa aatgttaaac aataccaata
    69361 cccataaatt agatagaata ccaataaaat ttcctccata agagagattt gtattttgaa
    69421 caggattgaa tcttaaaaat tttccagtta tatcaatttc aaagcccatt aagaataatc
    69481 ctataagaac tttgctaatt tggtctaaga atattaagga tataactgga aaaacaaaag
    69541 actttacttg tctgatatca acacctcctt gcaaaagtca ttcacttttt ccttactcag
    69601 tatcgttaaa ccccaaaaag gaatttcaat ttattaattt cacatcaata attataacat
    69661 gggtaaatta tcaagtcatt tcttttctga tttcgtaata tatttctact cgtaatcgtt
    69721 gtacaaattg ttgcaaatca tgctatcaga cttagcattt ctttgtttgc ttctgcctct
    69781 gacgcatggg cgtataagtc cagcagtctt gctttcactt aatacggttt tgggcttctg
    69841 tcgtcctcaa tgacttcgct tagttggaaa tcaaaaagat tcttccttac atagtcgtcc
    69901 tcgattgtca tgtagaatga cgcttttaag gaacacttgc tgtattataa aaagcagagt
    69961 tgcttcatat tttcttacgt gtcaaaaagc ttccaataat cgcccctata aggacaatcg
    70021 gtgttaatct gacaaataga cactcaaacg catgaaaagc tactccaaaa acagcggttt
    70081 cagggttggt ataattccaa ttcaagtaag gactatttag tcctcctaag accataaaaa
    70141 ctattactat gactgaacat aataaaataa ataaccaatg aaacattttt cttctcatat
    70201 tttttctttg tacaagttgc aagtttataa tctctaattt ttcagaaatc acttttaagt
    70261 tatcaacttc caactcaaca atagtttctc caagtaaagt gcttaccggc gtttcaagta
    70321 cctctgatat agaaatcaac atatcagaat caggaactga caatccttgc tcccatttag
    70381 atattgtttg tcgcactaca ttcaatttta tagcaagttc ttgttgcgaa agtccttttg
    70441 actttctgat tgttttaatg ttttcgctta acattagggc taaccacctt tctttcagtt
    70501 gttaccgata ttataggcaa aactgcttgt tgcttcaagc aacgcatttt aacattcaac
    70561 tgaaactccc acgcctaaag gtgtgtggtt tttttagata ctacatgaag ttctacaata
    70621 ctcttaatta taataaatca taacctacaa catattctca ctaatcatat tacctaagac
    70681 ttttattaac aaaatatctg tcgacaccat taacattaat attaggctcg tttctcaacc
    70741 tatatagttt ttatatcttc atactagata ttaatttata accatttaat tttaattctt
    70801 ttaacctatt tatttcttta tcatgtaata ttttaaagtt atcatatgtt ttaacacaca
    70861 attctctgtt gattgacttt aaattatcat taacattcat tattaaaaat gcataatagc
    70921 aatctctttg aataacatat cttctaagcc ttctatatta aatatttttc ttctatatct
    70981 gggacagaat acaaagtgat aattaattaa actgacagtt gtttgagttt tctatattta
    71041 ttctccatat acttatatta acattatatg tgtatgtatc aatacttttt atacactatt
    71101 tatgatatcc ataccacatc taaaggagtg ggctttccga actattctgt aaatttctta
    71161 tttttccttt aaccgctttg cttatatata cagtgtttat acggatattc tcgattttta
    71221 agcgcttttt gtaataaaaa tagatatgtc cagtaattaa aactgtagaa atatttataa
    71281 aggcataaga caaatttacc tacaattttt agtgtttaga ttctaaaaaa taaaaataat
    71341 tataattgag ttcataattt taaaacttat tgaatataat tatatgtcgg ttaaagacgt
    71401 atgaaaataa aaggaattta taagttatgt gttgtataga tactgtatat gcagcatgta
    71461 ataaatctta gcactattta tattctatac aaaaagatat ttgaagggag attaaaaatg
    71521 ggaatattag aaggcgctgc aatgataaga gagattgcaa ttaaaatagc taaagaaaaa
    71581 ggtattacag aacaaaaggc atggcctgaa gcggtaaaag aatttaaaga aaaatatgaa
    71641 cttgtattat aaactttgac tgtaaaactg tctctatttt attgagacag ttttttatat
    71701 tataacattc atattatgaa gaataatata tataatttta gattccttgt aactttaact
    71761 taaagtaaaa taatttagaa taaaaacagt attaataaat tttatttgtc atataaattt
    71821 gtttttaata ttatttttta aggtataaat cctatatatt agatataaat tttggaggta
    71881 tatgatttgg ataaaaaact tgaaaataga tgctcatggc aagaatattt tatgagactg
    71941 tgtgaaacag tagctgaaag aggaacttgt gatagagctt atgtaggagc tataattgtc
    72001 aattcagaaa acagaatagt gtctactgga tataatggta gtatttctgg ggataagcat
    72061 tgtagtgaag ttggtcatga aatgagagat ggacattgta ttagaactat tcatgctgaa
    72121 caaaatgctc tttattattg tgcaaaagaa ggtatttcgg ttaaagattg ttctatttat
    72181 gtaactcact ttccttgttt aaactgtact aaggctataa ttcaatctgg aattaaacat
    72241 atatattata gaactggata tagaatagat gagtatgcta taaaattatt gcaatcgagt
    72301 aatgtcttat atacaaaatt ataactttaa cactatttaa ataatattct atatttttac
    72361 aaaataaatt ataataaaag tgtaagatat aaaaagaaac aaaattctaa gttatgcata
    72421 tattaaatag aagttaaatt taattgtaaa ggagatgcta atttgaaagt agtaataata
    72481 ccagggcaca ctttaactgg aaaaggtaca ggagctgttg ggtatataaa tgaaagtaaa
    72541 gaaacaagaa tactgaatga cttaattgtt aaatggctta aaataggtgg agctactgtt
    72601 tatacaggaa gagttgatga atcaagtaat catttagcag atcagtgtgc tatagcaaat
    72661 aaacaggaaa ctgatttggc tgttcagatt cattttaatt caaatgtcac aacttcaact
    72721 ccaggaggaa cagaaacaat atataagact aataatgaaa aaacatatgc agaaagagtg
    72781 aacactaggc tggctacagt atttaaaaat aggggcgcaa agtctgatgt tagaggcctt
    72841 tattggctta atcatactat tgcaccagca atattaatag aagtatgttt tgtagatagt
    72901 aaagcagata cagattatta tgttaataat aaagataaag ttgctaaatt gatagcagaa
    72961 ggtatactaa acaaatctat atcaaattct caagggggag gtgtaaataa agtgtatgaa
    73021 aatgtaatcg tttatacagg tgacgctgat aaagtggctg ctcagatttt acattggcaa
    73081 ttgaaggatt ctttaataat tgaggcgtct agctataagc aaggtttagg taaaaaagtt
    73141 tatgttgtag gcggagaagc taataaactt gtaaaaggtg atgtagtaat aaatggtgct
    73201 gatagatatg aaactgttaa attagcatta aaacaaatag ataaattata aaattatgat
    73261 tattttataa aaaataaata agtcaggata aagaaaaaat gataagaaca aaagttgtag
    73321 agttaatagc aacagtatgc agagaaaaca aaccacataa atgggttgat gaaaattata
    73381 ctccttatga taaaagtggt aaagttgaat taatgtcaat agaagattta aatgagttaa
    73441 tatcaagcaa tggaaaagca gatttacttt atagttgtag attacaaaaa attctaaaag
    73501 agatttatat aaatcaaagt agagcatctt atatgtcagg ttgtggatta ttttggagtt
    73561 cgtattggga tatacttgaa gaaaaatttg aagaatggtt atataattct tatatctttt
    73621 ttgatgaaga tgatgaatat ttagaaggaa tggaagattt tgagcttgaa tgtaaagatg
    73681 ttcttatgga tgtaattgaa acaacaagca tagatatata tgttcaaatg ataaagagga
    73741 atattaccaa ttattaaaaa cacatagtgt caaattaagc taataaaacg accttataaa
    73801 tactatttta aggtcgttta aattatatct cttagacttc ttttatatta aacttattat
    73861 tttttatcta gaaaaatatt tttaattata gtcaatatat caactataac atccatattt
    73921 gatttaacac tgtctttagt aacaagaaaa tctgctgtaa cttcagtaac tacctctgtt
    73981 acatctttca tattttctcc tacttcgcca aaattttcta caacactcgg aagattattt
    74041 aaagttgtat ttatattttg agtattttca tttaacatag ttccaacagt atttaaaatc
    74101 ttatgtagtt taacaagtac aataattaaa taaactaaag caatacaagc gagtatagct
    74161 aataataaat atattatact tatattaatc gttattgtat tgttcatatt attcctcagt
    74221 aacactaact ggttcaacaa tatctattat tttatcttcc tcactagaat tttcaacact
    74281 ttttttgtta tctatatatt tttttatttt tttctttgat tctataaagt ttcctttttt
    74341 attctctagc ttctcagata ctttgccttt tacatctaca gttttcatat ttatcttttc
    74401 agcaatttgt tgtgaagcat cttttatatc ttctcttgtc tcttttccag atttaggagc
    74461 caaaagtact ccactcactg caccagtaac agctcctaat actacaccag ctgtagcaat
    74521 tttagctttc ttaacttttt cagctctctg tttagctttt cttttatctt ctattttctt
    74581 tgatagattc atgtttaatc gccccttatt ataatatttc taaacattat aatacatttt
    74641 ttaccataat gatatagttt ctaactaaat tcttaaaaat caataaaaat tctttttaat
    74701 tacttactat ccattacaat ttaaattttt ataaggattt tctatatatt tttattttat
    74761 tagtatataa tatgtatcac atatagatta taaatatctt atttaattag tttcatgtat
    74821 ataactaaaa attttttagt ataaatgacc ttcaatcttt ctatatagat taaattagta
    74881 tctcaaaaaa caataagaga ggaattaatc ctctcttttt aatttaatta gatttggcag
    74941 ggcggcgtat cctgcatctc tattaactac tctatagtag cgtattgggt gccttttcca
    75001 acctcaaatc taactaataa atatctacat gtatattaat attataacct atatgataat
    75061 atttttcaat attttcgggg aaattaataa tattatgtta accatcatat tgttttactc
    75121 ttccactgtt tattctactt tctaatttcg cttcttttat agcatacatt gaagagacat
    75181 ataaatattc tttttttgta tctaattctg tagccactaa aatataatca tcaatttttt
    75241 ttataaactc cacacttttc ccaggttttt taggatgact tcctatatat tcaggtgact
    75301 ttaataccat ttttatagta tctattatat cattaaaaat atccttactc agttgacatc
    75361 tatgtttttt ctttatatgt tttataacac cagaagaagc ataaatttta ccagtatatt
    75421 caattcctgc aagttctgcc gtttcttcat ctatgattcc aacaactttg tctgtagaca
    75481 tataaagcac attcctttct aagataattt actgttaata ctgtaaaatt tatttaaatt
    75541 aaatatccat atttaaagta tagtttattc caaaaagaaa agatagtata atatagtttt
    75601 ttcatatatg actttatata atcccttttt atataattta taaatggcaa ataaggttct
    75661 attttcaagg tatttttaga aactgtttaa tttttttaag ttttattgag tactcttgct
    75721 tctttataaa aatataatct aatacctata ttatataaaa cgttttcttt taaagttttc
    75781 tgtgataata ttatttatat ataaaataaa tttctaatag gaggaaatca tgaacaatca
    75841 acaacaagaa gtatataaaa aattagatga attaaacata ccttatgagg ctgtaaatca
    75901 tccaccagtt tatacaattg aagaaatgga agaattaaaa acattacata tggaatttgt
    75961 tgtaaaaaat ttatttcttc gtgatgctaa aggaaaagaa cattttttag ttgtattagg
    76021 taaagacaaa aaagcagatt taaaagatat tcgtcaacaa attgatagta aacctcttag
    76081 ttttgcatca gaagaaagac ttcaaaaata cttgaaactt actaagggag ctgtaactcc
    76141 tttcggtata ttaaatgata aagaagctgt tgtaaaagtt gtatttgata aggatttatt
    76201 aaaaatggat aaaataggta tacatccaaa tgataatact gctactgtat ttttaaaatt
    76261 tgaagatatg aaaaagctta tagaagaaaa tggaaacgaa atatactacg tagaagtata
    76321 attgcaactt aaaaaatggc aaaagcaatg aatgcttttg ccttcttact actgctgtaa
    76381 tcaatgctat taatataatc atttccatta gtataattat tttctacaat aatgattatt
    76441 tatgtttact tatctttttt tgcagataca agtagcatca taggtctacg tagctcatct
    76501 ttcataccag gaattgcatc tagcagtttt tcttctggtt gaggctcaat aatttctaat
    76561 acttcaaatc ctgttttaat taaagtattt aaataagttg ttaatgtttt atgatatttt
    76621 ataacctctt ctcctagaaa atttgactta cgtaatcctt cttcaaaata tctatctact
    76681 ggccagtgta gattattacc atccttatca cagtgccact cctgacttcc atgtgcagta
    76741 aatataggat gttcaacaga aaatacaaaa tcacctttat ttgaaagaca attacttata
    76801 tttttacata tatcttcaaa tgattgaata taatgaaatg ctaaagaact aataaccaca
    76861 tcaaaactat cttttggaaa attaatatct tcaataggca tacatatgta ttttatatta
    76921 tcaaatgttg ttttgtttct agcttctttt agcatttttt ctgaaatatc aattccaaca
    76981 gcagaagttg ccccattctc aactgcatgt tgacaatgcc atccaaatcc acatcctaaa
    77041 tctaaaactc tttttccctt gaaattaggt aacatttttc taaaagtatg ccattctcca
    77101 gcacctgcta gtccatttat agaacgttcc attttactgt atttattaaa aaatttatca
    77161 tcatcatatt tattttgttt cataaagctc cctccatttt aaatatat
//
LOCUS       DAMJVM010000007        70065 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.007, whole genome shotgun sequence.
ACCESSION   DAMJVM010000007 DAMJVM010000000
VERSION     DAMJVM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 70065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70065
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.007"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            29..406
                     /locus_tag="PC523_000540"
     CDS             29..406
                     /locus_tag="PC523_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2474103.1"
                     /translation="MMIRKIPQAELKVIKFIWRVDNTVTSKDVIEVMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDKYTNYAIIVKHKDYLSFETKDFLSNIHDDSLKSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            399..1433
                     /locus_tag="PC523_000541"
     CDS             399..1433
                     /locus_tag="PC523_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HDF2474104.1"
                     /translation="MNNYMIYVFDNLIKTTIVCSLGICLLLFLKRYVFKKFSKRFNYY
                     IWVVIVFRMLLFLFNYTVIYEVKEPKENIIGNNLIRIDISIDNNFMLYLAYLWLVVTI
                     VIAVYTFVKYTRFKNLVVDVSYDIEDNDVNCIYKNLLKKLNIKKKIELRSSDELISPA
                     GMGLFKSYIFLPDYPYSKDELNWILKHELMHFKNKDILIKFLVFSVKTIYWFNPLVYI
                     MSNKVNLDCELCCDESVLMDCSLEDKKEYALALIKSIKLSKNYNSGILTTEFNKTSLE
                     KRLDSIVKKKGKSGILVLLISFALFSVTYFNFESISFDNSNSLRNIGILEKTGADLKE
                     EDDISVRVVN"
     gene            1495..2097
                     /locus_tag="PC523_000542"
     CDS             1495..2097
                     /locus_tag="PC523_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HDF2474105.1"
                     /translation="MKSLKKILIVTGCLTIISTSLAFASTNNDVNVSKSNEDQATNSF
                     TITGYEFTYENAPQPIKSDYEKKCKELNINPSKNDTIFINEKFIDSYNINIDDYNIEP
                     KAFIIRYKKDLNRFDVTGPISYSVNINTTVVGYGHIEKGNPVHLVQLLCQQINGSSIN
                     PDSKFGPDTYNQVKILQGRLGVTKDGVVGKATWQAAGERY"
     gene            complement(2231..3247)
                     /locus_tag="PC523_000543"
     CDS             complement(2231..3247)
                     /locus_tag="PC523_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2474106.1"
                     /translation="MNYNDLLKSARENFNEKCKVCKICNGLACAGNVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTLLNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNCGNGIVFIKPWNNSKIIEKI
                     RLSEEAGAFAVGVDLDACGLINNQLQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMTVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKAVKGKITILVDGGVRSGVDV
                     VKMLGLGADAVLMGRPFVIASFGGGLDGVEFFIEKVRSELCETMILTACQNVKDIDGR
                     VIWK"
     gene            complement(3577..3759)
                     /locus_tag="PC523_000544"
     CDS             complement(3577..3759)
                     /locus_tag="PC523_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474107.1"
                     /translation="MYKDESDECIHLMTAYIDSLSGYYSFIDTQLEDFMVKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            4075..4494
                     /locus_tag="PC523_000545"
     CDS             4075..4494
                     /locus_tag="PC523_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HDF2474108.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYVFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIIQIENCIV"
     gene            4625..6466
                     /locus_tag="PC523_000546"
     CDS             4625..6466
                     /locus_tag="PC523_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2474109.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLNNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGVSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDINKANLYIDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIEA
                     LYIKIKKINDFNCKKMYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPMA
                     INNLAKSEVYLEKIKNQNLKEVAKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     IMKNIEEYTMYDLEIIYHQAKIYYIFGYYENALNTNKKLLEKSKNAKNYNYVKLAYKN
                     IYLCFEKIQDYEISMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYIDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITMIIKKYLKKDMLMIRYGGEEFLILSICKDYKKSKIFGRKLCRTVKKFLNNNL
                     TVSIGIDTCKNSSIDIREIIKNADKALYKAKESGRNRCLHYNDFKNF"
     gene            6508..8379
                     /locus_tag="PC523_000547"
     CDS             6508..8379
                     /locus_tag="PC523_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2474110.1"
                     /translation="MEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNTKLEDD
                     SRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKDDVYVISKIYIYLTLSAIS
                     IRKYDKANNFYFSAKKIIRQNHLNELLVLLNMSLCVEISALYQTKTDVIDLVEEVSFF
                     KNLNNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLRLAQENNLYSIEAFCKCIIS
                     LCYLESGNNVEAIKYINNVLDVAHNNNSISVTERVSIKIDLIWAYLKEDMNKEAEVIL
                     LKTIKLMKVVEGVYKDYLYSFIFLYSAEIELKKDNCSFEKVNTYLILSKNIYESFGDV
                     FIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYGLIANG
                     YELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLVDDNKK
                     LENDILKDGLTKLYNRRYLEQQLNLFNGTRDKNMVAILVDIDCFKSYNDNYGHLAGDK
                     VIVKVTDIIRSVFIDITEHIIRYGGEEILILVDSIESISVEEIDNIELYIKRVFKLLE
                     FEGIEHIYSKIKNYITISAGVSIKRCRSRKDVEMLIEDADKNLYKSKKAGRNQYIII"
     gene            8387..10387
                     /locus_tag="PC523_000548"
     CDS             8387..10387
                     /locus_tag="PC523_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2474111.1"
                     /translation="MILLQKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEAVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEIDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRNFKTKQSLSNSEVDKFNSETTASAEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            10649..11908
                     /locus_tag="PC523_000549"
     CDS             10649..11908
                     /locus_tag="PC523_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2474112.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKALGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINS"
     gene            11969..13756
                     /gene="iorA"
                     /locus_tag="PC523_000550"
     CDS             11969..13756
                     /gene="iorA"
                     /locus_tag="PC523_000550"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HDF2474113.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKDYVKNAKKMVTVPANAQIRRGVVEERMETLKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTSDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDNNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            13758..14333
                     /locus_tag="PC523_000551"
     CDS             13758..14333
                     /locus_tag="PC523_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HDF2474114.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELNRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            14386..15456
                     /gene="buk"
                     /locus_tag="PC523_000552"
     CDS             14386..15456
                     /gene="buk"
                     /locus_tag="PC523_000552"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2474115.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDNGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGKYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGNVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            15672..16373
                     /locus_tag="PC523_000553"
     CDS             15672..16373
                     /locus_tag="PC523_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474116.1"
                     /translation="MGKMNLKSMARLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVIIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVLMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSD
                     EHKCKSSIVIKNEQN"
     gene            16433..16939
                     /locus_tag="PC523_000554"
     CDS             16433..16939
                     /locus_tag="PC523_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDF2474117.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEDGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEESLEGAIREAKEEIGIDITKDEMKIFRSMTH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLSE
                     IDNNKLNI"
     gene            17112..18134
                     /locus_tag="PC523_000555"
     CDS             17112..18134
                     /locus_tag="PC523_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2474118.1"
                     /translation="MEDNKGLAHDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSYLNIPDSLIIIVLYALFAMLLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKKFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMRFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAIETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(18279..18587)
                     /locus_tag="PC523_000556"
     CDS             complement(18279..18587)
                     /locus_tag="PC523_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HDF2474119.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINEKNASCETFEMK
                     "
     gene            complement(18647..19075)
                     /locus_tag="PC523_000557"
     CDS             complement(18647..19075)
                     /locus_tag="PC523_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474120.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIKCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFTCSICGSTENVDVYHVNKNLGRETSKDVVVKCNKCICK"
     gene            complement(19296..20753)
                     /locus_tag="PC523_000558"
     CDS             complement(19296..20753)
                     /locus_tag="PC523_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF2474121.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGIIFLYYLCATLFPIDKIIGKIYPIFGAILLLSAAGVGVGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELIGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIVKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNM
                     NISYVLAAIFTVLYAILTVHSGRKLKSRADTKLAD"
     gene            21171..22085
                     /gene="nadA"
                     /locus_tag="PC523_000559"
     CDS             21171..22085
                     /gene="nadA"
                     /locus_tag="PC523_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDF2474122.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTILMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYITLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            22098..23399
                     /locus_tag="PC523_000560"
     CDS             22098..23399
                     /locus_tag="PC523_000560"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDF2474123.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMRAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFVDIPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            23380..24216
                     /gene="nadC"
                     /locus_tag="PC523_000561"
     CDS             23380..24216
                     /gene="nadC"
                     /locus_tag="PC523_000561"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDF2474124.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGNVGVELYKNDGDRVENREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSKYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGGVKNAVELARKNTSFVRKIEVEVETLDMVKEAIEAKADIIMLDNMSL
                     EMAKQAVDIINGRAIIEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            24474..24806
                     /locus_tag="PC523_000562"
     CDS             24474..24806
                     /locus_tag="PC523_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDF2474125.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPIGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            24968..25153
                     /locus_tag="PC523_000563"
     CDS             24968..25153
                     /locus_tag="PC523_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474126.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIVTKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            25181..26266
                     /locus_tag="PC523_000564"
     CDS             25181..26266
                     /locus_tag="PC523_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF2474127.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGSKVTIIYLISGFLVPFLVGFLGNCFGKEELSLHNDLEEEVIEL
                     EEEKRSFMENMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAAIVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            26259..26717
                     /locus_tag="PC523_000565"
     CDS             26259..26717
                     /locus_tag="PC523_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474128.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            26746..27831
                     /locus_tag="PC523_000566"
     CDS             26746..27831
                     /locus_tag="PC523_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2474129.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKQKEEAKAGKDDYTITVG
                     YYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGAIP
                     KGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLPTD
                     PSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKANGK
                     EYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEVAL
                     RTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGADD
                     FDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            27836..28060
                     /locus_tag="PC523_000567"
     CDS             27836..28060
                     /locus_tag="PC523_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474130.1"
                     /translation="MENIKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            28070..28633
                     /locus_tag="PC523_000568"
     CDS             28070..28633
                     /locus_tag="PC523_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474131.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPKKMGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            28594..29223
                     /locus_tag="PC523_000569"
     CDS             28594..29223
                     /locus_tag="PC523_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2474132.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFIYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIIPASLPDILTGSRIAISTGWMSVI"
     gene            29263..29400
                     /locus_tag="PC523_000570"
     CDS             29263..29400
                     /locus_tag="PC523_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2474133.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            29400..30152
                     /locus_tag="PC523_000571"
     CDS             29400..30152
                     /locus_tag="PC523_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2474134.1"
                     /translation="MKLSVRDINKTFVNNRVNTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSESGDVFINDKKVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEILLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            30165..30650
                     /locus_tag="PC523_000572"
     CDS             30165..30650
                     /locus_tag="PC523_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474135.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYISKK
                     LNMEKEYIESTLQYFIDKNIMVVDGDSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDVRINSICSVTGREIEIVIKNEKIEYVNNPSLRVLHINLDKYSNWAAN
                     C"
     gene            30657..30806
                     /locus_tag="PC523_000573"
     CDS             30657..30806
                     /locus_tag="PC523_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474136.1"
                     /translation="MNFFWTKSDFDAWTIEAGLSDDEDIYCLDINEAIVESYKIFKLK
                     QKIIL"
     gene            complement(30938..31759)
                     /gene="yidA"
                     /locus_tag="PC523_000574"
     CDS             complement(30938..31759)
                     /gene="yidA"
                     /locus_tag="PC523_000574"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDF2474137.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMTLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNDIEVKYIQKDKIDESIKICKIMIVDEP
                     EKLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCANLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            32253..33947
                     /locus_tag="PC523_000575"
     CDS             32253..33947
                     /locus_tag="PC523_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDF2474138.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLGCVEDINNSHNYKEQQKNIHKLN
                     LLRNKIETLSNIALIRYSTDTLNKFYQEEKDYWDEYMPLYEELNSHFYNAIVNSKFKC
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKIRELANKAYYNFFEKNETKFDDIFDKLVKLRDKISKKLGFEDFVEL
                     GYIRMMRSDYREDMIKSVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIQNGKRMYSELSKETNEFFNFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSAIKFIPYGVIVDEFQHYIYKNPNIDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLDRGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DTGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      34279..34389
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            34493..34864
                     /locus_tag="PC523_000576"
     CDS             34493..34864
                     /locus_tag="PC523_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HDF2474139.1"
                     /translation="MIIITNNPKVKEEVQDREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVVLKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            34960..35907
                     /gene="trxB"
                     /locus_tag="PC523_000577"
     CDS             34960..35907
                     /gene="trxB"
                     /locus_tag="PC523_000577"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDF2474140.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            35993..36310
                     /locus_tag="PC523_000578"
     CDS             35993..36310
                     /locus_tag="PC523_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HDF2474141.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
     gene            36577..37863
                     /locus_tag="PC523_000579"
     CDS             36577..37863
                     /locus_tag="PC523_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HDF2474142.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            37976..38449
                     /locus_tag="PC523_000580"
     CDS             37976..38449
                     /locus_tag="PC523_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:38108..38110,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HDF2474143.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            38481..39791
                     /gene="grdB"
                     /locus_tag="PC523_000581"
     CDS             38481..39791
                     /gene="grdB"
                     /locus_tag="PC523_000581"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:39528..39530,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HDF2474144.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            40038..41570
                     /locus_tag="PC523_000582"
     CDS             40038..41570
                     /locus_tag="PC523_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HDF2474145.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIVGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            41581..42726
                     /locus_tag="PC523_000583"
     CDS             41581..42726
                     /locus_tag="PC523_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HDF2474146.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFADIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            43032..44111
                     /locus_tag="PC523_000584"
     CDS             43032..44111
                     /locus_tag="PC523_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2474147.1"
                     /translation="MSKVKKVVELLESKGIDALYLTKKTNVNYISGFPDEEAYAVICK
                     YGNFLVTDSRYMELAENVCEGFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYIKDEEEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGEASEKQLEIYNLVKEAQNVGVENMKVGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEDGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            44222..44821
                     /locus_tag="PC523_000585"
     CDS             44222..44821
                     /locus_tag="PC523_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474148.1"
                     /translation="MYKKHKFIKNIKQAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSVNFIKRPSRKYINTDVSSNFFIDNNLSKKSQE
                     SSQNNVMNVKNTIGFTSETDNYEKKIPTNNTEDFEDGETSELQIVIATVICVVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSRPD"
     gene            complement(45001..45873)
                     /locus_tag="PC523_000586"
     CDS             complement(45001..45873)
                     /locus_tag="PC523_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2474149.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISSYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTKNLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            46290..47693
                     /locus_tag="PC523_000587"
     CDS             46290..47693
                     /locus_tag="PC523_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474150.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISPDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASTKDKEEILKVVDPEGKLDVSTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVINFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            47720..49264
                     /locus_tag="PC523_000588"
     CDS             47720..49264
                     /locus_tag="PC523_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF2474151.1"
                     /translation="MNNEGTFEELSGRLRNKKLCDLIMNADEAAKFIKDGMVVGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEDGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGIVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            49295..50686
                     /locus_tag="PC523_000589"
     CDS             49295..50686
                     /locus_tag="PC523_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDF2474152.1"
                     /translation="MGKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKIAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKAQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            50879..52375
                     /gene="abfD"
                     /locus_tag="PC523_000590"
     CDS             50879..52375
                     /gene="abfD"
                     /locus_tag="PC523_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HDF2474153.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSTDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKTIAGIKKEEALNL"
     gene            52510..52785
                     /locus_tag="PC523_000591"
     CDS             52510..52785
                     /locus_tag="PC523_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474154.1"
                     /translation="MTCGVRFCGGCNPRYERGKALAIIKEHFNGRVDFSIAQENLEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            52809..54116
                     /locus_tag="PC523_000592"
     CDS             52809..54116
                     /locus_tag="PC523_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF2474155.1"
                     /translation="MSWRELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPTFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSDFDYIVEKSQPLYELEPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFVNNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAIEFEKRFKEKY"
     gene            54136..55254
                     /locus_tag="PC523_000593"
     CDS             54136..55254
                     /locus_tag="PC523_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HDF2474156.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVPPKKIKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     AQIDEFTDSTIANQQRLLANNYVELNREEIREIFANLY"
     gene            55539..56459
                     /locus_tag="PC523_000594"
     CDS             55539..56459
                     /locus_tag="PC523_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDF2474157.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLIIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFVPTRYEDVAYEVKEEEK
                     TDEDNEVDTIINKGMNYLTHIKEANKNIKSESMCDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            56574..57731
                     /locus_tag="PC523_000595"
     CDS             56574..57731
                     /locus_tag="PC523_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF2474158.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTSDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(57819..59318)
                     /locus_tag="PC523_000596"
     CDS             complement(57819..59318)
                     /locus_tag="PC523_000596"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDF2474159.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
     gene            59913..61337
                     /locus_tag="PC523_000597"
     CDS             59913..61337
                     /locus_tag="PC523_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDF2474160.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWIIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TIAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDNTSLEEAKAKVSDMKSVSKNSKKDIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDVLFKRLESK"
     gene            61371..63500
                     /locus_tag="PC523_000598"
     CDS             61371..63500
                     /locus_tag="PC523_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2474161.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGMLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEESIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKSFREAYVNLIYDSFNEKEILDMVRNISENIQTDKTIEILSENRLLNLMDKCIIRK
                     VEGTLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMEIDFSMDIPLDEVGYITMHIRGAKQRSSSNHNALNLDDIEVMDITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYVYNGVE
                     KISRIIKDKLSINSIPESEIGYIAMHFASAIEKNLMMYANINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QLIKEKIKSIAQNKLINLNVKNGTKNKNKIYAQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCISERIDIMK
                     LSIIKLENRIILDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMILEVKDIVFNFYTNRLKAFIDK"
     gene            63530..63952
                     /locus_tag="PC523_000599"
     CDS             63530..63952
                     /locus_tag="PC523_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2474162.1"
                     /translation="MNKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            63971..65122
                     /locus_tag="PC523_000600"
     CDS             63971..65122
                     /locus_tag="PC523_000600"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2474163.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVEAEIITTAVGPLVLTKIASTIAK
                     GIKNRKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYIGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFIYDIVKSAMVESGEGLIKKYNFDSEAHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYDLSVDNLILGIGAALHYN
                     NSEDAQSVELQELIKSNGVKKAVAKIANINNDEELLNNIEKSYIFMENL"
     regulatory      65228..65329
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            65512..66084
                     /locus_tag="PC523_000601"
     CDS             65512..66084
                     /locus_tag="PC523_000601"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDF2474164.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFEGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANNVRLESLAVVESIDNGTVKFR"
     gene            66148..66798
                     /gene="fsa"
                     /locus_tag="PC523_000602"
     CDS             66148..66798
                     /gene="fsa"
                     /locus_tag="PC523_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDF2474165.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            66950..69874
                     /locus_tag="PC523_000603"
     CDS             66950..69874
                     /locus_tag="PC523_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2474166.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKS
                     GIKDKDLASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCENEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSQIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTIG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKDHIEKQIKEYDASIEVKPIAFLNNNLEDELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT        27577 a         7581 c        12664 g        22243 t
ORIGIN      
        1 ggtagcaaga gttttgaggg aggtgtaaat gatgataaga aaaataccac aagcagaact
       61 aaaagtaata aaattcattt ggagagtaga taatacagta acatcaaaag atgttattga
      121 ggttatggaa caaaaatatg gctggaaaca aactactaca ctgacacttt tatctaggct
      181 ggtaaaaaga gggttcttag atgctcaaaa aatagataaa tatacaaatt atgcaataat
      241 agtaaaacac aaagattatt taagttttga gacaaaggat ttcctaagta atatacatga
      301 tgattctttg aaaagtttaa tttcagcatt acatgatgat gaaaatatag gtaaagataa
      361 gctagatttt ttagaaagtt attttgataa tttaaaagat gaataattat atgatatatg
      421 tttttgacaa tcttataaag acaactattg tatgtagtct tggtatttgt ctattattat
      481 ttttgaaaag atatgtgttt aagaagttta gtaagcgatt caattattat atttgggtag
      541 taatagtctt tagaatgtta ctttttctat ttaattatac agtaatatat gaagtaaaag
      601 agcccaaaga aaatataata ggaaataatc ttatacgaat tgacatttct atagataata
      661 attttatgct gtatttagct tatttatggt tagttgtaac catagtcatt gcagtttata
      721 catttgtaaa gtatacaaga tttaaaaatc ttgttgttga tgtatcttat gatattgaag
      781 ataatgatgt aaactgtatc tataaaaatt tgttgaaaaa attaaatata aaaaagaaaa
      841 ttgagttaag aagttctgat gaattgataa gtccagcggg gatggggtta tttaaatcgt
      901 acatattttt gccagattat ccttatagta aagatgagct aaattggatt ttgaaacatg
      961 aattaatgca ttttaaaaac aaagatattt taataaagtt tttagttttt tctgttaaaa
     1021 caatatattg gttcaatcca cttgtttata taatgagtaa taaagtaaat ctagattgtg
     1081 aattatgttg tgatgaaagt gttttgatgg attgttcttt agaagataag aaagaatatg
     1141 cattagcact tataaagtca attaaactta gtaagaatta taatagtggt attttgacga
     1201 ctgaatttaa taaaacaagt ttggaaaaga gacttgacag tattgttaag aaaaaaggta
     1261 aaagtggaat attggtgtta ttgatttcgt ttgcattatt ttcagttaca tattttaatt
     1321 ttgaaagtat tagttttgac aattcaaatt ctttaaggaa tataggtatt ttagaaaaaa
     1381 caggtgcaga tcttaaggaa gaagatgata ttagtgtgag agttgtaaat taacatattt
     1441 ttgcagtgtt aaaagaacat tataatttat taacaaatta ggaggaattt tgaaatgaaa
     1501 agtctaaaga aaatcttaat agtcacaggg tgcttgacta taatatctac tagtttagca
     1561 tttgcaagta ctaataatga tgtaaatgta tcaaaaagta atgaagatca agcaacaaat
     1621 tcgtttacaa taactggtta tgaatttact tatgaaaatg caccacaacc tataaaatct
     1681 gattatgaaa aaaaatgtaa agaattgaat ataaatccat ctaaaaacga tacaatcttt
     1741 attaatgaaa aatttataga ttcatataat attaatatag atgattataa tattgaacca
     1801 aaagcattta ttattagata taaaaaagac ttaaatcgtt ttgatgttac tggacctata
     1861 agttatagcg taaatataaa tactactgta gtagggtatg ggcatataga aaaaggtaat
     1921 cctgtgcatt tagtacaact tttatgtcaa caaataaatg gttctagtat taatccagat
     1981 agtaaatttg ggcctgatac atataatcaa gtaaaaatac ttcaaggaag attaggtgta
     2041 acaaaagatg gtgttgttgg aaaagctact tggcaagctg ctggagagcg ttattagaaa
     2101 agaggttgtc gcactaagaa atagggcggc aaccttttta tatagagtat attaatcagt
     2161 atcttctaaa aatagttgtt attatatcta aagagtctgg aaatataagt gttataaaac
     2221 tatttcacaa tcacttccat ataactctac catctatatc cttaacattt tgacaagcag
     2281 tcaaaatcat agtctcacac aattcacttc taaccttctc tataaaaaac tccacaccat
     2341 caagaccacc accaaaagaa gcaataacaa agggtcttcc catcaaaaca gcatcagcac
     2401 ctaaaccaag catcttaacg acatcaacac cacttctaac accaccatca acaagtattg
     2461 ttatttttcc ctttacagct ttagctatat ctggtagtac ctcacaagta ccaggggtat
     2521 aatcaagtac tcttccgcca tgatttgaaa caataatagc actagcacca gattctacag
     2581 tcatcaaagc atcatctaca gtcataattc cctttataat aaaaggcaat ctagtagact
     2641 caaccaattc tctaatctca tcaattgtct taggagaaaa aggattttct tgaagctgat
     2701 tattgataag tccacaagca tcaaggtcta caccaactgc aaaagcacct gcttcttcag
     2761 acaatctaat cttttctatt atctttgaat tattccaagg ctttataaat acaattccat
     2821 ttccacaatt atcttttaac acatctaaat tatccagtaa aaaagtatct acattggtat
     2881 cgccaaccat agcataaatt ccagaattag aacaacctct aacaacaggt tctatgtatt
     2941 ctttttcaga aactttacca cccatgttca ataaagtacc tgatacaggg gcagcgaata
     3001 taggagaact catttttcta ccgaataatt ctatagatgt atctggcttt gaaacattat
     3061 gtattactct catatttatc tttatttttt ctagactctt tctattttca atgaaagatg
     3121 aaccacttcc tttaccaccc ataccaggaa catttccagc acaagccaat ccattacaaa
     3181 ttttacatac tttacatttc tcattaaagt tctctctagc tgattttagt aagtcattat
     3241 aattcataca aaatctctcc tttttataat attaaaatta agcattacta tattgaaaat
     3301 atcttagttg taaatataga gatgatgtta tgatgagata aactagataa tacatattag
     3361 atatttttta ttgttgcaga tagttaaatt atatcatatt agatagtaaa attttgaaaa
     3421 aaactaactt atttaagtat acattttaaa aaaattaaaa aagctatata tagattttta
     3481 aagaaatcta tatatagctt ttaagtttat gacaatcttt ttttattaat gaataaataa
     3541 tatagaatac aatgtacaaa aaagcttctt aaaatgctaa gataatcccc atttacaaag
     3601 caacatcatt atactatgca agtttgaatc aacaatgttt tcaccatatt tcaccataaa
     3661 atcctctaat tgtgtgtcaa taaatgaata atatccactt agactatcta tgtatgcagt
     3721 cattaagtgt atacattcat cactctcatc tttatacata tatatctcca cctatataca
     3781 atatataaat ttagatacta aatcgtatca tattaaatag ttaagtatgt cacatctatg
     3841 tagttatata tttatatcct tatattaata actaaagtga attttgacaa tagattttgg
     3901 gaaactggat gttcaaaatg taaacttgga ttttaaaatg attaattaaa atagtgcata
     3961 gtatatatgt atacataaat taattaatta taaagattat aaacaattac ttttattaac
     4021 tttatttaag tggtagaata acttggagat taataattat aaggaggata taatatgagt
     4081 ttatataagt ttatatatga tgataaggaa tatttattaa aagaggagaa ttgtagtgct
     4141 ttaataaatg atgaagaaaa tccagttcaa ggagtaagta tttcaaaaat aattgatata
     4201 cttaatgaag cagaagaagt tgattttgat gtagaatatt atcaagaagc atgtccatta
     4261 tgtttagaag gggtaaagga aaagaaaaaa ttctttcctt ttttggaata tcacttttat
     4321 gttttcagta aagatggaga atatgtaata agtaatatat ctgttgatta taaaggatta
     4381 tcttttaata aactatcaag ggcaaataaa gttgataata gttatattgt aagtgtgata
     4441 atttgtgaaa attgtcagga ttatataatt cagatagaaa attgtatagt ttagtgtaaa
     4501 gtatgtctta atttaagaga aatttctaaa ttgagacatc gacttttttc aaaaatagta
     4561 tggaaaatta ctaaatttat gagataatgt aagtcggagg tgagttacaa tatatagtaa
     4621 atatttggaa gagatagatg atacaatttt agattcaatg tttaagctaa ccttgaatga
     4681 aagattattg aataataacg attttattga aaataaaatt gatgtagtaa agaatttaat
     4741 cgattgctat aaaaccgaac aaaatagttt gatgttatta aaatcaaatt ttttgctagc
     4801 tttattatat ggtattcaag gggttagtga aaaaatgaaa gagtatattt tgatttcctg
     4861 taaatatatt gataaatgct tacccaaaga ttataagttt ctagcacgat tttattcaca
     4921 aatagctatt ttaagtttaa aaaatgaaga tataaataag gcaaatttat atatagataa
     4981 atttaatctt atttgtgatg aaaataattt tctaattgaa gaaattattt ttaaatcaca
     5041 acttatttat attaaggcaa gtaagtgtat agaatcttct gtaataatta aagaaataga
     5101 agctttgtat ataaaaataa aaaaaattaa cgattttaac tgtaaaaaga tgtatttttt
     5161 tattattggt aaaatatatt ttttattttt agatgatgct ctaattgcta aggtgaattt
     5221 tttaaaagca agaaaatatg cagatttact aaaggatatg caagtatctt cattatgtaa
     5281 tataaagata ggcgagtgtg aatgttcatt tgaaaattat gaatgttcga tggaatattt
     5341 caatgaagta ataagcaata aaaagtatag aaatgtaaat ataattcaaa aatatagagc
     5401 atcaaacaat ataactaaaa tattaataaa gactagaaat tatcctatgg caataaataa
     5461 tttagcaaaa tccgaagtat atttagaaaa aattaaaaat caaaacttaa aagaagtagc
     5521 gaagtttgat ttatttataa gcttagctat gtattatgca aaaagtaatg aaaaatcttt
     5581 tgaacaatca atctgctatt tagatagagc taaaaacata ttaaatataa tgaaaaatat
     5641 agaagaatat acaatgtatg atttagaaat aatttatcac caggcaaaga tatattatat
     5701 ctttggatat tatgagaacg ctttaaacac aaataaaaaa cttcttgaaa aatctaaaaa
     5761 tgctaaaaat tataattacg tgaagttagc atataagaat atatatttgt gctttgaaaa
     5821 gattcaagat tatgaaataa gtatgaaata ctttaaaatg tattatgagt taaaaatgat
     5881 gtatataaaa gctcgaaata gaaattatat agattcttta aattatagac atgagtatat
     5941 agaaaaagaa ataaataaca tgagaaaagt aaaacttgat ttagataaaa aaaagtatat
     6001 tgaccattta acaagtgcat ataatagaac ttttttgaac gattttttag aaaaacaaga
     6061 agttagtgaa tattgtacag catttatgat agatgtagat tactttaaag aatacaatga
     6121 tacttatgga cactataatg gagatattgc tcttaaaaat ataactatga taataaaaaa
     6181 atatctaaaa aaagacatgc ttatgataag gtatggagga gaagaatttc tgatattgtc
     6241 tatatgtaaa gattataaaa aaagtaaaat atttggaaga aaattatgta ggactgtaaa
     6301 gaaattttta aataataatt tgacagttag tattggcatt gatacatgta aaaatagtag
     6361 tatagatata agagaaataa ttaaaaatgc tgataaagct ttatataaag caaaggaaag
     6421 tggtagaaat agatgtttgc attataatga ttttaagaat ttttaactgt tgaaatagga
     6481 ttggtgattt acttgaaaag gagtttaatg gaagatttaa aaagaaatat aaacaagata
     6541 gaatacgata tgttgactaa attctatcta aatttaacag acttaaacta catcttggat
     6601 tatcacaaag accttttaaa tacaaaacta gaagatgata gtagagtata tactttatat
     6661 ttaattagtc ttataaacta tataaaaaaa gatagagaag catcatacaa ctatttgata
     6721 gagtcattaa agtatattaa gaaattgtca ttaaaggatg atgtatatgt aatatctaaa
     6781 atttatattt atcttacttt atcagctatt tctataagaa aatatgataa ggcaaataat
     6841 ttttatttct cagcaaaaaa aattataaga caaaaccatt taaatgagtt gttggtcttg
     6901 ttaaatatga gtttatgtgt agaaataagt gctttatatc agaccaaaac ggatgtaata
     6961 gatttagtag aagaagtgtc tttttttaaa aatttaaata ataaagctct tgtaagtagg
     7021 gcaaattata tatttgggag agtttatcta tatttattta ataactttat aaaaagtatg
     7081 gagtacttaa ttgaatgcct aagactggct caagaaaata acttatactc tatagaggct
     7141 ttttgtaaat gtataataag tttgtgttat ttagagagtg gaaataatgt agaagctata
     7201 aaatatataa ataatgtatt agatgttgca cacaataata actcaatatc agtaactgaa
     7261 agagtgtcta taaagataga ccttatatgg gcctatttaa aagaagatat gaataaagaa
     7321 gcagaagtta ttctattaaa gactataaaa ttaatgaaag ttgttgaagg tgtatataag
     7381 gattatttat attcatttat atttttatat tcagctgaaa ttgagttgaa gaaagataat
     7441 tgtagttttg aaaaggtaaa tacctattta attttatcaa aaaatatata tgaaagtttt
     7501 ggagatgttt ttatatataa ttctaaacta gattacatat ataagttgta tggagattta
     7561 tatataaaat ttggcaatat agacgaaggt ataagttgtt atataaaagg tttaaattta
     7621 gttaaaaaga gttcattaaa tcttaggaaa atctctatat tttatgggct gatagcaaat
     7681 ggatatgagc taaaaaaaga ttttaaactt gcaatagaat attataaaag tatggattgg
     7741 tattctgata gatgggaaaa aaataattcc tataaaacat ctcaagcaat tcacaaacaa
     7801 tatgaactta gacaaaaaca agagagttta agattattag tggatgataa taagaaatta
     7861 gaaaatgaca ttttaaaaga tggacttact aaactatata atagaaggta tttggaacag
     7921 cagttgaatt tattcaatgg aactagagat aaaaatatgg ttgcaatact ggttgatata
     7981 gattgtttta aaagttataa tgataattac ggtcatctag caggagataa agttatagtt
     8041 aaggtaactg atattataag aagtgtattt atagatatta ctgaacacat aatcagatat
     8101 gggggagaag aaattctaat actagtagat tctatagaat caataagtgt tgaagagatt
     8161 gacaacatag aattatatat taaaagagtt ttcaaactgc ttgaatttga gggtatcgaa
     8221 catatatatt ctaaaataaa aaactatata actataagtg ctggagtaag tataaaaaga
     8281 tgtcgtagta gaaaagatgt tgagatgtta attgaagatg cagataagaa cctatataaa
     8341 tcaaaaaaag caggtagaaa tcaatacatt ataatctaaa attgaggtga ttttattgca
     8401 aaaaaaaata ggaattatag cctctgatat tgaattaaaa gaaagaatag aagaattata
     8461 tagagaagat gtagaaaatg ggactataat aatcgacata cttaacctag acttaatgga
     8521 aaatcaaggt cgtatacttg ttgaaaaagg tgctcaagcc ataattggta ggggtggagg
     8581 ttatagttta gtaatagata cagtaaatgt acctgttata ccaatgaata tgaagtcaac
     8641 tgacctttta agagcaatag aaatagcaaa gaaatattct aaaaaagtag tattaattct
     8701 tggagacaat gaagtttctt ttgactatgt tggctggaga aatgtcataa gtactgaaat
     8761 aactgaagaa tggtttgaat ctaagtatga gataagaagt aaagttgtaa aatatataga
     8821 ccaaaaagat gaagttgtaa tcgtaggtgg aggtttggca tgttcttttg caaggcaata
     8881 tggaattgat agtgtatttg caactgctag tgatgaatct ataagagaag ctgtagaata
     8941 ttgtaaaaag ttgttggata cattaggtga agaaaaattt aataatgaag tgttacgaaa
     9001 tatacttgat ggtataaaag atggagtaat tgctatagat agtaatggta gtataatttt
     9061 gtacaatgaa agcgcaaaaa atatgcttaa agtagagaga aaatgtgcgc ttaataaata
     9121 cattttagat gtatttccta aaatggagtg gatgttagat tgtttacatg aaaaagaggc
     9181 tgtagaagat agaaaaataa gaaatataaa taatttaatt gttaatacta gaacgacatt
     9241 gataaaagtt gataatagta cttatggagt tttaggaata atacaagata taaccaagct
     9301 acaaaactta gaaagaaaaa taagatttga cttgaatcaa aaaggtcttt atgctagata
     9361 tacttttgat gattttttat ttaaagataa attaactaaa gaatttattg aagaagccaa
     9421 aaaaattgga aaaagtgatt atacaacttt actttatgga gaaagtgggt caggtaaaga
     9481 aattatagcc catagtatac acaatataag taaaagaaaa gatagaccct ttgttgccat
     9541 aaactgtgct actatagcag aaaatttact tgaaagtgaa ctttttggat acgaagaagg
     9601 tgcatttact ggtgctagaa agggtggaaa aagaggattg tttgaacttg ctcatggagg
     9661 tactcttttt ctagatgaaa taaatagtct atcttttaat attcagacaa aactactaag
     9721 agtaatagag gaacgtcaaa taatgagaat tggttctgat tatataatac ctctagatat
     9781 aaggattata gctgcaacga atgaatcttt aaccgaaaaa atagttatgg gtacctttag
     9841 ggcagactta ttttatagat tgagtagttt agagataaac atcccacctt taagagacag
     9901 aagagaagat ataatacctt tgtttaacaa ttttgtaaat gaagtattga aggatgatgg
     9961 tctcaatgga ataaacagta tagatgaaaa ttttgttttg actaaagatg aaattgataa
    10021 gctatataat tatagttggc ctggaaatgt aagagagtta aagacaatag ctcaaaaata
    10081 tgttgtgaca ggtaaaatta aattgagaca agataggaat tttaaaacta aacaatcact
    10141 ttctaactct gaagtagaca aatttaattc tgaaacgaca gcaagtgcag aagtccaaga
    10201 tgagtctata aatataagta aaataaatga tggtaagata agtatagata taaaagaagt
    10261 aaataagtat gttgaagaaa aaataataag tatgcttttt gctcaggggc tttctaaaaa
    10321 tgaagttgca caagttcttg gaataagtag gacatcttta tggaagaaat ataataaaaa
    10381 tatttaaata taattacata taaattattt tgaagtaatg ttaatttttt taaacaagtg
    10441 ttaagaaaaa ttaacattat ttttttgcaa atttttattt ttttagacaa gcatatatat
    10501 ttataagaaa aatagctaaa acacttaatt taataatgaa aaaaataata attctaaaca
    10561 aaatcagttt tacataaagt ggaatttatt ggcataataa ttgcttaata tataatgcaa
    10621 gagtaaatac aattttaggg gtgatggtat gagtcaaagt atggttggta agcatgcaat
    10681 gtggccaaaa gaaaatgatg ttatatttag catatctggt agagctcaag cagcagagaa
    10741 ggctctcggt atggataatg taataaatgc aacaataggc gcgctaatgg atgattcagg
    10801 aaaattaata acaatgaaaa ctgtatatga agaatataaa gcattagata actgtgagat
    10861 aggtgcatat gcagctcttg aaggtcaacc agattactta gaagcagtta aaaaagtatt
    10921 tttcagagat tacttaccag aaggacatat aagagtatta gcttcaccag gaggaagtgg
    10981 tgcaataaaa ttagctgtat ggaactatac taatgaagga gatgaggtat taacatctga
    11041 ttggttctgg agtccatatg taagtatagc tgaagaggct aatagaaaag tagttaatta
    11101 ccaacttttt gatgaaaata gaaggtttaa ttttgagtct ttcaaagaaa aatttgtaaa
    11161 tatagcagaa aaacaaggta gagtttttac aattataaac acaccagcac acaatccaac
    11221 tggatatagt gtcgcagatg atgagtggga taaaatatta gacttatcaa aagaagttgc
    11281 aaaagataaa gacaaaaaga taatattttt cgtagattca gcatatattg actttgcagg
    11341 tgatgatgat gtatgtagaa agttttttaa gaaattttct aatttgcctg aaaacgttct
    11401 cgtattggtt ggcttcagta tgtcaaaagg atttacagct tatggtatga gaatgggggc
    11461 tataatttgt atatcttcaa gtgaagatgt agctgaagag ttccattatt catgtgttca
    11521 ttcatgcaga gcaaattggt caaattgtaa tagaagtgcc atggcagtat tatctaacat
    11581 tgtaaatgac cctaaaaaat tcaaagaata tgaggatgaa aaagaaatat acaaaaacat
    11641 gcttactaga agagcagatg tatttgtaaa agaagctgaa agagtaggat tagaaattct
    11701 tccatatata gcaggtttct ttgtaagtat accatgtgat aatccaaaag aagtatgtga
    11761 agaattaact aaacataatt tatttgcagt tccattaaaa atgggattga gatttgcagt
    11821 ttgtgcagtt tctgaagata aatgtaagaa agcaccaagt attataaaag aagcacttga
    11881 aagtttagaa gtaaaaataa atagttaatt tcattgaaca aattgggtgg actatgaatt
    11941 taataattgt agatggaggg aattttatat gaaacaattg atgacaggta atgaagcaat
    12001 tgcacgtgga gcctatgaag ctggtgttaa atatgcatct gcatatccag ggactccaag
    12061 tacagaaata ttagaaaata tagcaacata taaagatgca atagttgctg aatgggctcc
    12121 aaacgaaaaa gtagcacttg aggctgctat agggggttca attgcaggag cgagaacaat
    12181 ggcttctatg aaacatgttg gcttgaatgt tgcagctgat ccaatattta cttatgcata
    12241 tacaggagta aatggtggta tggtacttat tacagcagat gaaccgggaa tgcattcatc
    12301 tcaaaatgag caagacaata ggatgtatgc aaaatttgca aagatacctt tatttgaacc
    12361 atcaactagt caagaagcta aagatatgat aaaagaagct tttgaagtta gtgaaaaata
    12421 cgatacacca gttttatata gagttacaac aagactctgt cattcaaagg gattagtgga
    12481 gtgctatgat agagaagaag ttgaaattaa agattatgtt aaaaatgcta agaaaatggt
    12541 aactgttcca gcaaatgctc aaataagaag aggcgttgtt gaagaaagaa tggaaacact
    12601 taaaaaattc tcaaatgaaa ctgatttaaa ctactatgaa ataaatgata caaaaatagg
    12661 tgttatagct tctggtatgt gttgtaattt tgccaaagaa gtctttggga aaaatgcatc
    12721 atatatgaag ctaggattca caaatccatt accttatgaa aaaataaaag aatttgcaga
    12781 gaaagttgat aaaatctatg ttatagaaga aaatgaccca tttatagaag aacaaataaa
    12841 agcatatgga atagattgta ttggaaaaga tgtaatacca ccatatggag aaatgacacc
    12901 agatgtttta agaaaagcta tatttggaaa aacaagtgat actatagaat acaaatctga
    12961 attagtaaca ccaagaccac caagtttctg tgcaggatgt ccacatagag gatttttcta
    13021 tgaacttgga aagagaaaaa atttaatagt tggtggagat ataggttgtt atactcttgg
    13081 atttgctcct ccatataatg gaatagatta tgttgtttgt atgggttctg cttttggaac
    13141 tgcacatgga gctcaaaaag ttcttaatat gaaggataat aatgaaaaaa gattagtagg
    13201 tgtacttggc gattcaacat tcttccatac aggaataaat gggcttttag atgttgttta
    13261 caatagagga aattctattt cagttatatt agataataga ataactggaa tgacaggtca
    13321 ccaagaaaat ccaggttcag gatatacttt acaaggtgct aaaactaaag aagttgatat
    13381 agaaggatta gttaaagctt gtggaataga acatgtaaga gttataaacc ctaataattt
    13441 aaaagaagta aatgaagcgc tagattgggc tttagctata gaagatgaac catcagtaat
    13501 aataacaaga tggccatgtg tacttaaaaa attctctaaa gaggatatag aggaatttaa
    13561 taatccattt aagactaagt gtaaagttga ccatgataaa tgtataggat gtaaattatg
    13621 tttgaaaact ggatgtccag cactatcatt tgataaagag aataagttat caaatataga
    13681 tagaaaccag tgtgtaggat gtggagtatg tgcacaagtt tgtccaaaac aagcaatagt
    13741 taaggaggaa aaataatatg actaagagta tacttttggt aggtgtagga ggtcaaggaa
    13801 ctatacttgc tagtaaatta ttaactatgg gattgatgga agctggatac gatgtaaaaa
    13861 tgagtgaaat acatggaatg agtcaaagag gaggttcagt ttcctcacaa gttagatatg
    13921 gagattgtgt tcattctcca gttatagaaa ttggtggagc agatatatta gtatcttttg
    13981 aaaaaatgga agctttaaga tggttcaatt atctaaaacc agaaggaaaa gctttagtaa
    14041 ataatcatag gatagattct atgactgttt taatcggagg agctgagtat caagaaagtg
    14101 atatagatgc ggagttaaat agattaaatg caaaagttat aaatgcagct gataaagctg
    14161 aagaactagg aaattcaaaa ataatgaatg ttattttatt aggatgtcta gttaaatcta
    14221 tgcaattaga gtctattgac tgggaaaaaa ttataagtga aaatgttaag ccaaaatttg
    14281 ttgaattaaa cataaaagct ttccatgaag gtatggaaat ggttagcaaa tagtttataa
    14341 tagatataaa tagactatag aaattaaggt gaaaggaaga aaattatgac ttacagaata
    14401 ttagccataa atccaggttc tacttctaca aaaatagcag tatatgatgg agaagaacaa
    14461 attcttgtga agacgataga ccatccagct gaagagattg caaaatataa tactatacaa
    14521 gaccagtttg aaatgcgtaa ggaagcagtt ttgaatattc ttaaagaaaa tagtatagat
    14581 ttaaaatctc ttagtgcaat agtaggaaga ggtggagttt taccaccagt aaaatcagga
    14641 gcatatttag taaatgaaga aatgattgat gtacttagac atagaccagt gcttgaacac
    14701 gcttccaact taggtgctgt tgtagcacat gcaatatcag aacctcttgg aataaattca
    14761 tatatttatg attctgttgc agtagatgag cttatagatg tagcgagaat atctgggctt
    14821 tgtggaatgg atagatcaag tgcagggcat gcattaaata ctagagcaat ggctttaaaa
    14881 tatgctaagg ataatggaaa agattataag agcttaaatt taatagtagc tcatattggt
    14941 ggaggagtaa gtatttatct tcatgaaaaa ggaagaatgg ttgatatgtt atctgatgat
    15001 gaaggaccat tttctccaga aaggtcagga agagtacctg ccacaaaatt agtggctgcc
    15061 tgttattcag gtaaatactc agaaagagaa atgactaaaa agataagagg taaaggtggt
    15121 atagtttcat acctaaatac tgtagatgct agagaagttg aaaaaatgat agcagaagga
    15181 aatgaagaag caaaaattat ttatgaagca atggcttatc agttagcaaa aggtattgga
    15241 gagttagcaa ctgtagtaga tggaaatgta gatgctataa ttataacagg tggaattgca
    15301 tattctgaaa tgtttacttc aatggttaaa aagaaagttg agtttatagc acctgtagaa
    15361 attatggcgg gagaaaatga gttggaatca cttgcttttg gaactttaag agtactaaat
    15421 ggagaagaag aagctagaat ttatagtgaa aattaataag aaaaactaaa aatgtttata
    15481 tactaaaaaa gtggtatatc cgttttttat acacgaaagc ttagagtata ggtgaaaatg
    15541 tataaagttt ggattgccac tttttataat taaaatttta tcttaagcta ataaataatt
    15601 ctgtaaaatt tcgtatttat atatatttta ttggtattta ttgttattat tattatgagg
    15661 agtgattttt gatgggaaaa atgaatttaa aaagtatggc aagattattt tcaggttttt
    15721 ttatatttgc aatttcttct gtgcttatga ttaatgcaca tgtaggtctt atgccttggg
    15781 atgtacttca tcaaggttta tctataaagc taggtataac tataggtcag gcttctataa
    15841 tggtaggagt cataattgtt atattggatg cagtttttgg tgaaaatata ggatggggta
    15901 ctttactcaa catgacgttt ataggaattt ttattgattt agtaatattt tctggagtga
    15961 tacctcatgc aagtaacact tatataggtg tgcttatggt tgttatagga attattttgg
    16021 cagctatagc aagctttcta tatttaggtg tttgtctagg aagtggaccg agagatgggc
    16081 ttatgattgc tcttcagaaa aaaactaata aatctgtacg tcttgttaga acaattcttg
    16141 aaatactagc attggttgtt ggttggttat taggaggttc tgtaggtatt ggtactcttg
    16201 taagtgcttt aggtttagga tatgtattac aaatagtgtt tagaatattt aaatttgata
    16261 caaaattact aaaacataga tttattatag atgatataag agaatggaag gaaaagaaaa
    16321 gtgatgaaca taaatgtaaa tcaagtattg taataaaaaa tgagcagaat taattatgct
    16381 aaaatatatt tatagtttat caaagttata gtaaaggcaa ggagactttg aaatggaatt
    16441 atgggattta tataatgctg atggaattaa aacaggaaat gttataaaaa gaggtaattc
    16501 tattgaagat ggttattacc atttggcagt tgaagtgtgg attttaaata gtaactctca
    16561 aattttaatt cagaaaaggt ctaaaagtaa aaagacattg ccaaatatgt ggggaatgac
    16621 aacggggtgt attgtttctg gggaagagag tttagaagga gcaattcgtg aagcaaagga
    16681 agaaataggg atagatataa ctaaagatga gatgaagatt tttagaagta tgactcatga
    16741 agataccctt tgggatgtat atttggtgaa aaaggaatat gatatatcca aagccatttt
    16801 acaggaggaa gaagtaagtg atataaagtg ggtaagcaca gatgaaataa gacaattact
    16861 aaaagaaggt ttattttttg aatatccaga gatatacgaa ttgctatctg agatagataa
    16921 taataaatta aatatatgat gtagagcaga tttcagtata agtatgatta aaaatttatc
    16981 ttctctaaat tttaatattg ttttatgaaa attaataagt tatttttagt atttttctac
    17041 ttgtttttta agattaaaat aaaaaagggc ggtcattgta aagtcataaa aatttgggga
    17101 ggctttaaac tatggaagat aacaaaggat tggcacacga cagttcaaag tataaaattg
    17161 tttctatatc tgttgtagta attattctaa ttacattttg gtatatgtta aatatggtgc
    17221 ttttaacatt cattatgact tttattttct ataacttgct tgtagccaca agaaaacgaa
    17281 ttaagaaatt ttcttattta aatattccag attcattgat aattatagtg ttatatgcac
    17341 tatttgccat gttgttggta ttaattagtt atgcagttgt accaataatt atagttcaac
    17401 ttacagagtt gagtagggtt tttagtgatt ttgatgtaaa tcaatttgct caatctttag
    17461 gaccaaaact gtatcctatt gtttcaaagc tagattttaa taaatatatt tcacaagcag
    17521 ggctactcat agcatcaaca gcgacaaaag ttggaagttt tggagtaaat atattattag
    17581 cttttttgtt gagtttactt cttttacttg aaaaaaatga aattaaaaag tttggagata
    17641 aactttctga tagtaagatt tcttttattt ataattcgct tgtatttttt ggaaagagtt
    17701 ttgttaaaaa ttttggtgaa gtaatgaaag tgcaagtgat gattgccttt ataaattctg
    17761 ttgtttctat gattttttta ggtttcatga gattccctca aatatgggcc ttaggattta
    17821 tgatttttgt gttaggtctt attccagtag caggagttat tgtgtctttg attccattaa
    17881 ctgtaatagc atttaataca ggaggaataa caaaggtttt tggagtttta cttatgattt
    17941 gtatagtgca tgcaatagaa acatacatat tgaaccctaa gttgatgtca aatagaacta
    18001 aacttccagt ttgttttgtt tttatcattt tacttgtagg agaacactac ttaggtgtat
    18061 ggggattact aattggagtt ccaatattta tgtttcttat ggatattcta ggtgttaaat
    18121 ttacttctag ataatatatt aaaataataa tttattttta tatatattat gaattgtttt
    18181 aaaaaagaac ctataaatag acatcattaa taaagatatc tatttgatag gttcaatttt
    18241 gatttttata aatactataa acttaaatga aaagaaattt atttcatttc aaaagtttca
    18301 caacttgcat ttttttcatt tatatgaact ttttcagctt tgcaaagttc atttttattg
    18361 tatttacatt ttacagcttc acaatgaatg ttatctgtat caacttggtt agtttcatta
    18421 gaacgactag ttattccaga tgtatatttg tcttcaaaag aagcacaata agtattttct
    18481 gtagtagttg cttgcattcc atctactttt atactaccag cataacagta tccactagta
    18541 ttataagcac aattagtagc agcacaattt aaattattat ttttcataaa taacctccta
    18601 agttttattt aaacttatta tgtgaagtta tctaaatttt atacacttat ttacaaatac
    18661 atttattaca tttaacaact acatctttgg aagtttctct tcctaaattt ttattaacat
    18721 gatatacatc aacgttttca gttgaaccac atatagaaca tgtaaaatta ttttctagta
    18781 gagttctttc tttaaataac ttccaataca tagaattgat gtaatcatta taatttacct
    18841 tgaggcatgt ctcaaggaag tttatgtata gattgtctaa atctcctgta tttaactctg
    18901 tatattctgg ttcaaaatat tcacaacatc ctctaaaatc aggctttgta tctgaaataa
    18961 cacatttaat atcatatcta tagtctaggc taggcccctt aaatgaaaga tgtgtgcaaa
    19021 aagaacaaga tgtttctggt agattttttt caagttcatg taatttatta ttcataagta
    19081 ttcctccaaa aaatttattt taagtataaa tgaatagatt attttaaaac ttttatttat
    19141 ttatattaga aatataggat aaatattcca ataaggagta aatacttaaa tgtttatttc
    19201 tgtattaaat aaaaaagagg caaatctaac ttgagattgc ctactttttt attacttttt
    19261 aataaaattt aatctaaata ttattttatt tacctttagt cagctaattt agtatctgct
    19321 ctgcttttta actttctacc agagtgtaca gttaaaatag catataatac agtaaatatg
    19381 gcagctaaaa cgtatgatat attcatgttt aagttaaatc caatttgagc attaaatata
    19441 taactaaata taacaaaaga ataaaacata cctggaatta agcttataat ataattcttt
    19501 tcttttacta taaggtaaac tgtaatcatt gcaaacgcaa atatggcgat agtttgatta
    19561 ctccaagaga agtatctcca caatatatta aatccagagg cactaagttt tgcaaatata
    19621 agaatggcta tgacaggaat aaatatacag attgataata taacacgatg tttcttttcc
    19681 ttttggtcat aatgaaagta atctgcaagc attaatctta aagatcttaa agctgtatct
    19741 ccagaagtta ttggtaaaac aattacacct attatagcta tgataccacc aatagagcca
    19801 agtaaatctc ttgcaactaa acctattaca tcaggagaac ctattaattc cttaggtatt
    19861 cctttattgt agatacccat agcagcagca gcccaaatca ttgcaattag accttctaaa
    19921 atcatcatat tataaaaagt agtctttcct tctttttcat ttgaaactga tcttgctata
    19981 agagtagctt gagttgagtg gaatccagaa acgataccac aagcaactgt aacaaagaat
    20041 gtaggaataa gaggaatccc atcaggatga atacctttcc aatttgcaag acttaaatta
    20101 gccaaatcat aaccttgtgt aaagattcca actccaacac cagcagcaga taaaagtagg
    20161 atagctccaa atattggata tatctttcct atgattttat caattgggaa tagtgttgca
    20221 cataagtaat atagaaagat aattccatag actatccaga caactggatt atttatagtc
    20281 gatttcatat ttaaaatttg agtaacaact aaatctccag gtgtataaat aaaaactgca
    20341 ccgactaaaa gcattaaaag acaaaggaat atattataga cttgaaatac tttattacct
    20401 aaaaatctac ttataagaga aggcatttgt gctccttttt ctcttatact aatcattcca
    20461 ctcatatagt catgtaaagc tcctccaaat acacagccaa taggtattag tatgaatgct
    20521 attggaccaa acaatatacc ttgtataggt cctagaattg gtcctgtacc agctatatta
    20581 agcaactcaa ttagactatt tttccatttt ttcattggaa catagtctac tccatcactt
    20641 tgagcaagtg caggtgtttt tctgtcatct gggccaaata ctttttcaca ataagcaccg
    20701 tataaagcac ctcctattag taatatagcc aagccgatta agaatgttgt cataatatca
    20761 ccccatttat aaaatttttt ggtctttgta tacatattat ctaaatgtgt agatttatat
    20821 gacatttaat tctggacaac ctacaaattc tataaagtat taaaactaat ataatttcag
    20881 aaataaatac atatacataa ccatgtactt agtatacagg tatttgatat tattttcaat
    20941 attatatcaa taaagaatat aaacaaaatt ctgataatat tttacaaaaa aattaaaaaa
    21001 tataaaaaat agtttaaata gatgaggaaa aaaggaaaat gaattgatac tgtaaatgtc
    21061 tatatcttaa caatattaag aggtttaaaa ttcttgaaga aagaatagtc aagtgatata
    21121 atgagtttac aggtgtatat acactagtac atatagaaag cggtgcaatt atggataaag
    21181 atttaacata tcaaatcaaa gagctaaaga aagaaaaaaa tgcaataata cttgcccatt
    21241 tttatcaacc accagagata caagaattag cagatgcagt tggtgattca tattatttaa
    21301 gtgagattgc tagagattgt aaagaagaag tggttgtatt ttgtggagta aggtttatgg
    21361 gagaaagtgc taagatatta tcacctgaaa aaactatatt gatgccagta agtaatgctg
    21421 ggtgtgctat ggcagatatg gtagatgaag aaggcgtgat aaaattaaaa caacagtatc
    21481 caaatgcttt ggttgtatgt tatataaact ctacagctaa agttaaggca cattgtgatg
    21541 tttctgttac atcttctagt gcaattaaaa tattagaaaa catagataat aaagaaatta
    21601 tattccttcc agataaaaat ttaggaggat atattgcaga acaatttcca gataaaaact
    21661 ttatattttg ggacggatat tgtaaatacc acaacaatat aagggctgaa gaaataattg
    21721 agttaaaaga taagtataaa aatgcagaag tattagtaca tccagaatgt aaaaaagaaa
    21781 taagagattt aggagattat gtaggtagta catcaggaat aataaaatat gctactaata
    21841 gtaaaaacaa agactttata atagcaactg aagaaggtat attacatgaa ttaaagaaaa
    21901 ataatcctaa taaaaatttt tatatccctg gtggaaaaat actatgtaca gatatgaaaa
    21961 agacaacatt agaaaattta tatattacac ttaaaaatat ggaaaatgaa gttattgtag
    22021 aagatgaaat tatggaaaag gctttaaatt cactgcttaa catgcacaaa ttagcagaag
    22081 gatagaggta tagacttatg aatttagaac aagatgtttt aatagttgga agtggagttt
    22141 caggattata ttgtgcatta aatttagata aaagtttaaa tgttctagta gtatcaaaat
    22201 caacaattga aaacaataat acatatcttg cacaaggtgg aatttcaact gctagaaata
    22261 ttgatgatat agaatccttt gtagaagata caatgagggc tgggcagtat aaaaatagag
    22321 ttgaagcagt tcaagtactt gcagatgaat ctatagaaaa tgtagggcag atagtagaat
    22381 atggtatgcc tttagataaa gaaaatggag agatagatta cacaagagaa ggagctcata
    22441 gtgtaaatcg aatagttcat agtaaggata atactggaga agttgttttt aaaactttat
    22501 taaaagaagc taaaactaga gaaaatataa ctcttataga agatgcttat cttttagata
    22561 tactaaaaga tggtaataaa tgtatagggg ctagaatttt taaatctaaa aaagagattc
    22621 atgttttttc aaaaatagta gttttagcta caggtggaat tggtggactt tttaaaaatt
    22681 ctactaatca aagacatctt acaggtgatg gaattgcaat agctttaaga aataatataa
    22741 aaatagaaaa tttagaatat attcaaatac atcctacagc tttttatgaa gaaaataatg
    22801 aaggtaggag aatgcttata tctgaatcat taaggggaga gggagctaag cttttaaata
    22861 aaaataaaga aagatttgtt gatgaacttc ttcctagaga tgtagtttca aaagctattt
    22921 ttgaacaaat ggaaaaagat aagttacctt atgtgtattt agatgcaaca catctagata
    22981 gtgagtattt aattaataga ttttctttta tatataatga atgcttagct agaggaacag
    23041 atataacgaa agaatgtata aaggtatctc ctgcacaaca ttattttatg ggcggtatac
    23101 atgtagattt ggattcaaaa acatctatgg aaaatttgta tgcagttggt gaaattagtt
    23161 gtacaggtgt tcatggagcc aatagattgg caagtaattc attacttgaa gggcttgtat
    23221 tttcaaaaag ggcagctaaa aatataaact cggttataga taatgtaaaa gttaagtttg
    23281 tagatatacc agatatggat atagatatag aacaggtaaa aaaagaaaat aaaatattag
    23341 tcataaaaga aattgaaaga acaagtgagg attttggtga tgaattattt gattattgat
    23401 agaatgatac aagatgcttt gatagaagat gttccaagtg aagatataac aacaaattct
    23461 attgtagatg aaaactctaa atctactgta gatttaattt gtaaacaaga tggtataata
    23521 gctggcttag gtgtatttaa gagagttttt gagatacttg gtaatgtagg tgtagaatta
    23581 tataaaaatg atggtgatag agtagagaat cgtgaaaaga tagctgtatt aacaggtagt
    23641 actagaaatt tacttgtagg agagcgtgta gcattgaatt atttacagag aatgagtggc
    23701 atagctacta ttacaagtaa gtatgttgaa aagcttgaag gtacaaatac aaaattatta
    23761 gatagtagaa aaacgatacc taatcttaga atattagata agtattctgt taaagtaggt
    23821 gggggatgta atcacagatt taatttatct gatggaatat tgcttaaaga taaccacatt
    23881 ggggctgctg gtggtgttaa gaatgcagtt gaattagcta gaaaaaacac ttcttttgtt
    23941 agaaagatag aagtagaagt agagacttta gacatggtaa aagaagcaat agaagctaag
    24001 gcagatataa taatgttgga taatatgagt ttggaaatgg caaaacaagc agtagatatt
    24061 ataaatggaa gagcaattat agaattttct ggaaatgtaa atttagatac catagaagat
    24121 ataggaaaaa tcggtgtgga tgttgtttct gtaggagcac ttacacattc tgttaaagct
    24181 ctcgatataa gtatgaagaa tttgagaaat atataaattt tatattgaaa taaatttaat
    24241 tgaaatagac tccttaaatt tgatttataa atttaaggag tctatttttt attcattaaa
    24301 attaataaaa atataaaaaa atcaatcaat aatttaataa aaatagataa acactatata
    24361 cacttttaat aatatagtta ttatctattt tacagataaa taagaataaa cttataatat
    24421 aattgtatct gtaaaatttt gtaaaaatat aaaaagtata ggggggaata aaaatgaaag
    24481 tagcttatat ttttagtagt actaatacac acaaaattct agacaaaatg ataataccac
    24541 aacttgaaca aggaactcat ggagtagatg ttctagggat gtttttcttt atggataata
    24601 ccctattttt atctaaaggt aatcctatag gggagagatt aagtaaatta catgaaaaaa
    24661 ctgatatgat aataatggct tgtgaccaat gtgctataga aagaggtata gagaataatt
    24721 tggttgatgg agctacaatt ggatgtttcc caaacctata tgcttgttta ggcagtgcag
    24781 gtgtggacca agttataacg ttataaatta gtgtaggata caatccatag aaaaatttat
    24841 tttctgtgga ttattttaag taaaagataa attgtttaaa gtaggggatg agcaaatgtc
    24901 taaaatttta gttgaattta taaacacttg accatcttgt gatgagcatg gagtgtttat
    24961 aaaaaaaatg tcagaaaagt atgctgataa attggaaata aaattatatc aagcaggcaa
    25021 agatttttca tatataaaaa aatatgggat tgtaacaaaa ggaactttaa taataaatca
    25081 gaaaaagaaa tatgatagat taaataaaga cacaattgaa agggcaatag tagaagctat
    25141 aaacaataat taaaagttta aaaattagga gtatcaaaaa atgaatatat tagaaattgt
    25201 tttaaatgga gttaaagata ctatagtagc ttccatagtt acattaaatg gagcatcagg
    25261 atggcttatg ataagtttta tattagcagg agtgcttcac aatgttttaa ctcctagaag
    25321 atttcaaaaa caacttggaa ataagaaatt ttcctctttg attaaaagta ctatctcagg
    25381 aatgttttta ccaatatgta gttgtggggt aataccactt ggaataagca tgtattattc
    25441 aggggcatat ttaggaccgg tacttgcttt tatgacatca actccaataa taaatccaat
    25501 agctgtagtt ctttgtctgg gtcttttagg gtccaaagtt acaattattt atctaatatc
    25561 tggtttttta gtaccatttt tagtaggatt tttaggaaat tgttttggaa aagaagaatt
    25621 atcacttcat aatgatttag aggaagaagt aatagaatta gaagaagaaa aaagaagttt
    25681 tatggaaaat atgattgatg gtcttaaatg gtctttcggg gagttggctc ttactattag
    25741 taaatatgta gtaatgggta tggtttttgc aggatttata acaaccattt ttccaaatag
    25801 tataattcaa aaatatttag gaaatccagg tttgttatct cttggaagta tagcaatact
    25861 tgcatgtgtt atgtatgtat gtgctgtagg acatataccg tttatagcag ccattgttgc
    25921 aagtggagca tctcctggga tagctataac atttctaata gctggagcag ctacaaatct
    25981 accagaactt ataagtatgt ttaaacttat tggaaaaaga actgttatta tatattctgt
    26041 aactttgaca ctgtcttcaa taataattgg atatataaca aatttacttt tagtgaacaa
    26101 taactcagta aatttagatg caacaaattc ttcaatacag ttggcaaata aactaatgtt
    26161 aaatatacct gatagttttg agtatgtatg tacgatttta attattttat ttgcatttaa
    26221 atcaataata ccaaagatga aagaggtcat aggaaatcat gcttaaaaag atagttataa
    26281 tatcctgtat agtagttgtg ttaatcattt tatcaaaaat agttgatgat aacatcaaag
    26341 aagatgcctc tattccaaat gtgaataaag aaacattaga atattttaga aaaaattata
    26401 aagaagacat aataacttgt gctgaagaag acttaaataa tgatgggaaa aaagatttag
    26461 tagttatata taaaaaaagc aataattcaa atgaaatggt agttgtggtt tctgataaaa
    26521 atagccatta tataacaaag ccaataccag cacctataga gaatcaaact ataaccttta
    26581 aaaatattga tgataaagca ccaatagaag taatagtatc agggtctaaa aatggtaatg
    26641 taggatatgc tatttacaga gtagagggta agaaatttgt tgatttattt ggcgaagata
    26701 tggacaaatg ttgctaaaaa taatacataa aggtgggaaa tttatatgaa attatctaag
    26761 aagattcaag cattaatctt atttggtgta atgggtacat ctatattaac aggatgtagt
    26821 agtaaacaaa aagaagaagc aaaagctgga aaggatgatt atacaataac agtaggttat
    26881 tataattgtg accatatgac tgcgggacca gtagcagaat cagcaggaat atataaagat
    26941 ttaggtctta atgtaaaaac tgttggaaat ggtaaagttc ctgaagctat ggcagcagga
    27001 aaaatggatg caggttacat aggaactaaa ggtttagttg gagcaatacc taagggttca
    27061 ccaataacaa tagcagctaa caaccatact ggtggttctg aatatttaat agtttcaaaa
    27121 gatataaaag aacctaaaga tttaattggt aaaaaaattg caacagatat gagtgatttc
    27181 ttatggacaa gtgattatgg tccagaaaca ggtttaccaa ctgacccatc aaaatatgaa
    27241 gttgtaaata tggattcaga caaagataaa tatttagctc ttaaaactgg gaaaattcaa
    27301 gcttttacat cttgtgaccc ttggggttct gtagctgaaa atgacggtgc aggaaaaata
    27361 atagcatcta ctcaatataa agaaaaagct aatggcaaag aatataactg ttgttcattc
    27421 tcattgaaca aaaactttat aaaagaacat ccagatttag caaaaaaatt ggttctagca
    27481 catacaaaag caatagaata tatatataca aatccagctg aagcagctaa aatatttgca
    27541 aaatattata atgtagaaga agaagttgca cttagaacta tctataagaa gactgttgga
    27601 gaaggtagaa ctcttacttg gaaagttaca ggtgaagaat ataaaaacaa tttacaaatg
    27661 tataaagatt taaaggcttt agatgatata cctaaatatg aagatacgat tgatacaagc
    27721 ttattagact cttgtggagc tgatgatttt gacaaattta taaaagaaaa agttaaccca
    27781 gagtttcctg aaggtatgag ttatgaagat tggaaagcaa aggtattata aataaatgga
    27841 gaatataaaa aatataataa tactttttat cattatttta ggaagtatat ttataatatt
    27901 ttttaatttt gaagataaag aagaaataaa aggaaaacta agaatagggg tatctgatga
    27961 tacctctggt tttgtaataa attatatggt aaatagagac tactttgaaa atataaaagt
    28021 aagtgatata atggatattt ttacaattaa tgattgctga gcaagtacaa tgcagtgggc
    28081 tctgagttca gaggaaatag atatagcaat catatgtaaa gatgctgcaa aacaatatgt
    28141 aaatgtaaat agtggatttg aaattgttgg tactgtagtt caaaattctg atatatttct
    28201 attaagtgat aataatccta aaaagatggg agtcattcaa aatagagatt accagtcaga
    28261 attggttaaa aaatactaca agaatgtgga agtagctccc ttattaggga ctgcacttgc
    28321 atatggatta gaaagtaatt tagtagacgg tgttgtaata gattgcataa agtcaattgg
    28381 gttagagggt aaaagaaaat ctacaactaa attaggagat tatgatacct atgtattagt
    28441 tgtaagtaaa gagtttaaaa aaagtaaagc atatagtgat tttataacac tttacaataa
    28501 aagtgttgat gctttaaaga ataaagctac atttaagaaa gcattaaaag agtacaggaa
    28561 tataagaaat atagatatgg aggaaataag caattggaaa ttaaaatttc taaagataaa
    28621 agcgatagat taactaagaa aaaaactgat ggtagtatgg atattttata taaaataata
    28681 ctattagtta ttatattttt agtctggcat tttgcagcaa aagatatagg gagttcttta
    28741 ttacttccaa tgccagtaga tgtaataaaa ggtttctttt tctgtgtgac agatgcagag
    28801 acagttacta atctttttat aacattgcag agagtactta aaggttttat ctatgcatta
    28861 ttgtttgggc ttccaatagg atttattatg ggattttcta aaacctttga aagggtttta
    28921 tctccagtag tagattctgt aagacaagtt ccaataatgg catgggttcc tcttactata
    28981 gtgtggtttg gtataggaga tggtccaaca atatttttaa tagctttttc aggggtcttc
    29041 cctataattt taaatactat tcaaggagta agagcaatat caaaggatta ttataatgca
    29101 gctagaagta tgggagcatc accaattgtg atatttacaa atgtaattat tccagcatct
    29161 cttccagata tactaactgg ttcaagaatt gctataagta caggatggat gtctgttatc
    29221 tgagccgagt ttatagctac gagtgccggt ctcggttact caatggtaca agctcaaaca
    29281 ttaatgcata cagatgtatt aattgggctt atgatatttg cagctttaat agggtttata
    29341 atagataggg tattaaagtt tataaataaa atcctttgta gatggaggtt tgcagattaa
    29401 tgaagttaag tgtaagagat ataaataaga catttgtaaa taatagagta aatacaaaag
    29461 ttttagaaga tataagtatg gatatagatg atggacaatt tgtctgtctt ttaggaccat
    29521 caggttgtgg aaaaacaact ttacttacta taataggtgg atttcaaaaa tcagaaagtg
    29581 gagatgtttt tataaatgat aaaaaagtta aaaaaccagg aatagataga gcttttatat
    29641 ttcaaaatta tgctttattt ccttggaaaa ctataagagg caatgttcta tttcctatga
    29701 aacagcagaa aataccaaaa gaaaaaagag aagaaatgct agaagaactt ttggttatgt
    29761 cagacttgaa aggaaaggaa aaactgtttc cacatcaatt atctggagga atgaaacaaa
    29821 gagtggccat gataagagct ttagcatgta atccagagat attactaatg gatgaaccat
    29881 taggggctgt agattttcaa atgagacaaa atttacagga agagctagaa cgaatatgga
    29941 taaagaagaa aataactgca ttaatggtaa ctcatgatgt tgatgaagct gtatatatga
    30001 gtgatagagt tatagtaatg tcgagagata aaggaagaat tattgatgat ataaatatag
    30061 atattccaag acctagaatt agaggaagcc aaaaatatga agagtacaaa aacaaattaa
    30121 ctgatacttt atcaaagtgt tatgaagttt aaagaggagt agacatgagt caaatagaaa
    30181 aatacaatag tttggataat acacaacaga aaataagaat ctctattatg gatatgataa
    30241 tagataaagg ttctagtgtt acacttcaag aagtgactga atatataagt aaaaagttga
    30301 atatggaaaa agaatatata gaaagtacac tacaatattt tattgataaa aatataatgg
    30361 ttgttgatgg agatagtata aattttatat atcctgtatc agcattgcca acaaatcata
    30421 aagtaacttt gagagataat agaagtttta gtgcaatgtg tgctatagat gctataggga
    30481 catcttgtac ctttaaccaa gatgtaagaa taaactctat ttgtagtgtt acaggtagag
    30541 agatagaaat agttataaaa aatgaaaaga ttgagtatgt gaataatccg agcttaaggg
    30601 tgcttcatat aaatcttgat aagtattcaa attgggctgc taattgttga aatataatga
    30661 atttcttctg gacgaagagt gattttgatg cttggacaat cgaagctggg ttatctgatg
    30721 acgaagatat atattgttta gatattaatg aggcaatagt agaatcgtat aaaatattta
    30781 aactcaaaca aaagataata ttgtagatat acataaaaaa ctaaaaatta cctttaggta
    30841 aaaatgatag ataatatcca tactgtataa attagtatag agtatggata tgaaaagtat
    30901 ttaaatctaa aggtatattt ttagtttttt tatatgatta gatagcctta ttaagtatat
    30961 atttatttat aacctttgca actccatttt cattattagt atcagtaaca taatctgcaa
    31021 tttcctttat actattacgt gcatttccca tagccacacc aagacctgca tactttatca
    31081 tatgttggtc attttcttca tcgccaacag caatagcatt ttctataggt atgtttagat
    31141 tagcacataa tgttttaagt gcagttcctt tattagtagt tttacctaaa aattctagat
    31201 aaaaaggagc agaccttact atagtatatt tattgaataa atttttaggt agctgttgta
    31261 tgatttcttc caatttttct ggttcatcaa ctatcataat tttacagatt ttaatagatt
    31321 catcaatttt atctttttgt atatatttga cttctatatc atttaatttc gcttcatgaa
    31381 gtgtatattt tccaggagtt tcatttggtg taattaaatt tgtcattgta tttatgtgta
    31441 ttttagtctt atatttttta caaacgttat tatatataaa atcaaaatca tctaaagtca
    31501 tttctacatc atgtataatt ttctcacttt taacctcctg aataattgct ccattaaagc
    31561 atataacata atccccttca tctgttaaat ttagttgatt tagaagctct tttacaccag
    31621 gaagaggtct gcctgttgtt ataactactt ttgccccagc cctttttgcc tcttgaatgg
    31681 actcaaagac ttcaggagta atcctttttt gtgtatttaa tattgttcca tcaatgtcta
    31741 gtgcgattaa tttgtacata tcaacctcca ataaattata tactcaaatt tcctgtgaaa
    31801 acgttggcac gactagtttt gaacaaagtt tcagtgggaa atatggtttg ttttgtatac
    31861 tattatatca agagataagt aatttagaaa gtttaataat attaattttt tgataaaaat
    31921 attacctatg tgagatgaga gtgtaaaaac tttgtaatca ataattccaa aatttttaat
    31981 tacaaataat ggtatcgttt gcattgttaa tataaatata atgtatatta tatagttaga
    32041 agtttgaata aatagactga tggtaatttg ttatattagt tagttttgtg ttataaaata
    32101 ttgtaattcc attaatcata tgttattatt caacatgatt gcaatataaa gaatatttaa
    32161 tatatataat aatttataaa agagtattac atagtacatg taaagttata tttataaata
    32221 caataaaata aagttgtaaa ggagtgtttt aaatgaagtt ttcagagttt aagtatgaaa
    32281 gacccaatta taatagtatg aaaaaagagt ttttgggttg tgtagaagat ataaataatt
    32341 ctcataatta taaagagcaa caaaaaaata tacataagct taatttattg agaaataaaa
    32401 tagaaacgtt gtcaaatata gcattgataa gatatagcac agacacactt aataaattct
    32461 accaagaaga aaaagattat tgggatgaat atatgccttt atatgaagaa ttaaattcac
    32521 atttttataa tgctattgta aattcaaagt ttaaatgtga tttgataaaa gagtttggag
    32581 aacaattttt cacaatagta gagtattctt taaaaagttt ttctaaagaa ataataagtg
    32641 aattacagga agaaaataaa ttgtgttcag agtatactag acttttagcc tcagctgaga
    32701 taatgtttga tgggaaaata agaaatttat ctggtatggg gaaatttatg tattctaaga
    32761 atagaaaaat aagagaacta gcaaacaaag catattataa tttctttgaa aaaaatgaaa
    32821 ctaaatttga tgacatattt gataaactgg ttaaacttag agataagata tctaaaaaac
    32881 ttggttttga agattttgta gagcttggat acattagaat gatgagaagt gattacagag
    32941 aagatatgat aaaaagtgtt agaaaacaag tattaaaata tgtggttcca atggctaatg
    33001 agctttatga aaaacaagct aaaagaattg gtctagaata tttaagttat attgatgaag
    33061 gtgtggaatt tttaacagga aatgcaagct taaaaggaga ttcaagatac ataattcaaa
    33121 atggaaaaag gatgtattct gaattgtcaa aagagacgaa tgaatttttt aattttatgt
    33181 tagaaaatga gcttatggat ttagaaacga agaaaggaaa aggagccgga gggtattgta
    33241 cttatatacc agattacaag tcaccattca ttttttctaa ttttaatcaa actgctgatg
    33301 atatagatgt tttaacacat gaggcaggac atgcatttca gttgtatatg tctagatgga
    33361 ttgatatgcc agaaattaat tttccaacgc ttgacagttg tgaaatccat tcaatgagta
    33421 tggaatttat aacatggcca tggatggact tattttttaa agaagataca gataagtaca
    33481 aatttactca cttatcatct gcaattaaat ttatacctta tggagtaatt gttgatgaat
    33541 ttcaacacta tatttacaaa aatcctaata tagataagtc aaagagaaaa gaaatctgga
    33601 ggtttttaga gaaaaaatac ttacctcata gaaaatatgg tgataattct tttttagata
    33661 ggggctgttg gtggtttaaa caaggacata tatttaaaaa tccgttttat tatatagatt
    33721 atgtattagc tcaaatttgt gcgttacaat tttggaaaaa aatgattcaa gatagagata
    33781 caggatggaa ggattatata aatatctgta aagttggagg aacaaaatca tttttagata
    33841 ttgtgagtat gggaaatttg tattctccat ttgatgatgg atgtattgag tctattatag
    33901 gtgatgttaa atcttggttt gatgaaataa atgacagcaa attataaaaa attatgagaa
    33961 ttttattgta taaaattaaa aaattaaaaa atatataaaa aacgattatt tgttgtgaaa
    34021 atagtcttgt ttttaactaa attgataaaa taaactaaaa aagatttgca cttaaaaaaa
    34081 aaagaaaata caagaaatat gcaatatcaa catttggaaa aaaaagtaaa aatgtataca
    34141 aaatacatat aaaaaaacta ttgcaaattt tatagaatgt ttatatactt aagtcaacag
    34201 agatagaaaa aagctattta tagaaaacaa aggataaatt gcttaatttt aaattttctg
    34261 aaaaaaataa taatccggat gaagagtatg ggagactgct ttataaaaag cgaccgaagg
    34321 gacaagttta atcttactta cccaaaagat tagatgaaat tctcaggtaa aggtgactgt
    34381 acttggacgg ggctctgaaa atgattttaa cagggaaggc aaattgggag atttgttttt
    34441 cctgtttttt atttatcaag aaataaataa aagtttgaaa gagagtaaaa aaatgataat
    34501 aattacgaat aatccaaaag taaaagaaga agttcaagac agagaagttt tgtttaaaga
    34561 cacaacttat attggaatct tagaagcaag tagagacttg atacatgaag gttatgaact
    34621 tttatctcat ccactctatg ggagtgtaaa accaaatgaa acaccttaca ggactgttgt
    34681 actaaagaaa ggaaatcgtt tggatataaa ctctttaacc ttaattgaag aagcaatcat
    34741 cactgcaagt aaatttcaga ataataaaaa gactccaaaa tggacagaaa gtgttcaaga
    34801 tgattttaga gtaattgatt atgatatatt ttataataca atacaaagaa tgcagtatga
    34861 ataaagaaaa ttgttaaaaa gttattaaaa cagcctaaat attaaatcta atttaatatt
    34921 aaataaaatg agaaatttaa ttggggagga taagtcaaaa tggaaaatgt gtatgactta
    34981 gtaataattg gttcaggacc agcagggctt gcagcaggtc tttatggagc aagagcaaag
    35041 ttaaaaactc taatcctaga aaaagacaag actggtggac aaattgtaat aacacatgaa
    35101 attgcaaatt atccagggtc agttccaaat gcaacaggac caagtttaat agctagaatg
    35161 gttgaacagt gtaaagagtt tggagcagaa atgctaagag acaacattgt tgatactgag
    35221 ctagatggag atataaaagt tttaaaaggg gaaaaggcag aatatagagc aaaagcagta
    35281 ataattggta caggagcaac accaagaaag attggttgtc ctggagaaaa agaacttact
    35341 ggtaaagggg tttcatattg tgcaacttgt gatgcagatt tctttgaaga ttttgaagta
    35401 tttgttgtag gtggaggaga tagtgcatta gaagaagcaa tgtatttaac taaatttgct
    35461 agaaaagtaa caattgtaca tagaagacaa ggatttagat gtgctaagag tgttgaagaa
    35521 aaagcaaaag ctaatccaaa gatagagttt ttattagata cagtaattga agaaatcaaa
    35581 ggtgatggaa tattagaatc agttgtattt aaaaataaag ttactggaga aacacatgaa
    35641 tactttgcag atgaagaaga tggaacaatg ggagtatttg ttttcgttgg attagatgct
    35701 caaactgatt tattcaaagg caaagttgat atggatgaaa aaggttacat aataactgat
    35761 gaagatatga gaactaatat accaggggta tttgcagcag gagattgtag atctaagact
    35821 ctaagacagg ttgtaactgc aactaatgat ggggcaattg catctatagt agcagagaaa
    35881 tatattgatg aaaaatttgg aaactaatat caaatttgga aactaatatc tgtaaattaa
    35941 atttaatgaa caatattaaa ataaaataaa aatttcaagg aggaataaga gaatgttaga
    36001 tttagataag gctacatttg aagaagaagt tttaaatgca gaaggttttg tatttgttga
    36061 tttttggagt gaaggttgtg agccttgtaa agctttaatg ccagatgttc ataaattagc
    36121 tgagacttat ggagataaaa taaaattctg taagatggat acaacaaaag ctagaagatt
    36181 agctataaaa caaaaagtat taggtcttcc aactatggct atatacaaag atggagaaaa
    36241 agtagacgaa gttactaagg atgatgctac agttcctaat atagaaaaca tgattaaaaa
    36301 atatctttaa tatgttgata aagtattaac agtaaaagaa aaatctattt ttctctgaag
    36361 ataagaagaa aaaagttttt atgtctaact ttgttaaaaa aataatttta aataaaataa
    36421 tttttttaca ataaacattt tttagtaata aaataaagat ttaagacata aaaacacaag
    36481 aatatatctt aaaaaaataa gataaattgt aaaatgatat aattttgaga aaaactcaaa
    36541 tgcatataca caaattaaat aacaggaggt gcaagtatgc gtcttgaatt agggaaaatc
    36601 tttataaaag atattcaatt tggtgatgta acagaagtaa aagatggagt tttattcatc
    36661 aataagcaag agatgctaca agaaattggt ggagatgagc atattaaatc tatagatata
    36721 gaattagctc gtccaggtga aagcgtaaga ataacacctg taaaagatgt tattgagcca
    36781 agagtaaaag ttgaaggtaa tgggggaatc ttccctggta tcatgtctaa ggttgacact
    36841 gtgggagaag gaaaaactca tgctttaaaa ggtgtagctg ttgtaactac tggaaaaata
    36901 gtaggtttcc aagaaggtat aatagacatg actggagaag gagctaagta cactccattt
    36961 tctaaattaa ataatgtagt agtaatagct gaacctattg acggtttaaa acaatacgct
    37021 catgaaaaag ctgttagaat gataggattc aaagcagcaa tgtacttagg agaagtagca
    37081 agaaatctta ctccagacga agtttcagta tatgaaacta aacctcttct tgaatcaata
    37141 aaagaatatc cagagcttcc aaaagtagga tatgtttaca tgcttcaaac tcaaggttta
    37201 ttacatgata cttatgtata tggagtagat gcaaaacaaa tagtaccaac tttattatat
    37261 ccaacagaat taatggacgg agcaattgta agtggtaact gtgtttctgc atgtgacaaa
    37321 aacccaagtt atgttcatat aaacaatggg gttgttgaag atttatatgc aagacatggt
    37381 aaagatataa actttgtagg tgtaattata actaatgaaa atgtttacct agcagataaa
    37441 gaaagatctt caaactggac ttctaaatta tgtaaatatt taggattaga tgcagttata
    37501 gtttcacaag aaggttttgg aaatcctgat actgacctta taatgaactg taagaagata
    37561 gaaatgcaag gtgttaaaac agttatagtt acagatgaat atgctggacg tgatggaggt
    37621 tctcaatcac ttgcagatgc agatgtaaga gctgatgctg ttgtaacagg tggaaatgcc
    37681 aaccaagttg tagttcttcc taagttagat aaagttattg gtcatttaga agtagtagat
    37741 gtaatagctg gtggttctga tggaagttta agagctgatg gaactattga agttgaaatc
    37801 caagctataa caggtgcaac taatgaaaca ggatttggtc atttaactgc taaaggatat
    37861 taagaagtat ttcattaaac gattgagtaa tgttaataaa acatattata gataaattat
    37921 aataagtgtt atagatatat aaaaatatat tagaaatata ataaggagga ttattatgag
    37981 tttacttagt aataaaaagg ttcttataat aggtgaccgt gatggtatac caggacctgc
    38041 gatagaagaa tgtgtaaaaa cagtagaagg agcagaggtt gttttctcat ctacagaatg
    38101 ctttgtctga acagctgctg gggctatgga cttagaaaat caaaacagag ttaaagatgc
    38161 tgctgataaa ttcggagctg aaaatgttgt gattttacta ggtgctgctg aagccgaagc
    38221 tgcaggtctt gcagccgaaa cagtaactgc tggagatcca actttcgctg gaccacttgc
    38281 tggagttgcc ttaggattaa gtgtttacca cgttgttgag gagccaatta aatcattatt
    38341 tgatgaaagt gtatatgaag accaaataag tatgatggaa atggttttag aagttgaaga
    38401 aatagaagaa gaaatgtctg gtataagaga agaattttgt aaattttaat gcctaaaagc
    38461 aaaaggggtg aaactaagag atgggaaaac taaaagcagt acattatatt aatcagttct
    38521 ttgctggtat aggtggagaa gaaaaagctg atactaagcc acatgtggca gaaactttac
    38581 ctccaataag tttacaactt gataaattat taggtgaaga tattgaaata gttggaacag
    38641 tagtttgtgg agatagctat ttcaatgaaa atattgatag tgcaagtgaa gaagttttat
    38701 caatggtaaa aggttttgaa ccacaacttt tcatagctgg tcctgcattt aatgctggta
    38761 gatatggagt tgctgctgga acaataacta aagttgtaaa agatgcttta aatataccag
    38821 ctttaacagg tatgtatgtt gaaaatccag gagcagatat gttcaaaaag gatgtttatg
    38881 tagtagaaac ttctgattct gcagcaggta tgagaaaagc acttcctaag atagctaaac
    38941 ttgcagtaaa acttgctaat ggagaagaaa ttggaactcc taaagatgaa ggatacatag
    39001 ctagaggaat aagagttaac tacttccatg aagacagagg ttctaagaga gctgttgaca
    39061 tgttagttaa gaaaataaaa ggtgaaccat ttgaaactga atacccaatg ccaaactttg
    39121 atagagttga cccaagcaaa gctgtaaaag atttatcaaa atgcaaaata gctttagtta
    39181 cttctggtgg tatagttcca aaagggaatc cagatagaat agagtcttct tcagcttcta
    39241 aatatggaac ttattctata gcaggagtta tggatttaac agaagagact tatgaaactg
    39301 cacatggtgg atatgaccca gtgtatgcaa atttagatgc agatagagta ttacctgttg
    39361 atgttcttag agacttagaa aaagaaggtg taattggaaa attacatgag actttctata
    39421 caactgtagg taatggtaca tctgttgcaa actctaaaaa atatgcttct gaaattggag
    39481 cagcattagt ggctgatggt gtagatgcag taatattaac ttctacatga ggtacttgta
    39541 ctcgttgcgg tgcaacgatg gtaaaagaaa tagaaaaaac tggacttcca gtagttcata
    39601 tgtgtacagt agtacctatt tcattaacag taggggctaa tagaatagtt ccaactatag
    39661 caatacctca tccacttgga aatccagcgc ttgaccctac agaagaaaaa gccctaagaa
    39721 gaggacttgt agaaaaagca ttaaatgctt taactacaga agtagatggt caaacagtat
    39781 ttgaaaaata aaaataacac acacatatat tatatagata aaaatataaa cacacactaa
    39841 aaataaaaaa taactataca caaaaaaata atatagattt catgaatgga taaattggag
    39901 ttattcattc atgaaatctt aaaaaatgcc aaaatacata aacaataata gaattaaata
    39961 tacaataaaa tacaataact agaattttta ataagattaa aaaataagtg ttcataaaat
    40021 ttatggaggt gcacaaaatg acttatccag ttttaaaagg ggcaggctat gtacttattc
    40081 atactccaga tatgatagtg caaaatggaa gtacttgtac agttgaaaga gcaacaaatc
    40141 cagactcaga atttttaaaa gaagtaagta atcatataag aagttatgaa gatgtagtta
    40201 actacatgcc aaatcaagtt tatataggaa atagaagacc agaagaatta agagatttac
    40261 caatgccatg gtgtgaacaa aagattgagg gaactagaaa tggtaagttt ggagaaataa
    40321 tgcctcaaga tgagttcata gctttaatgc aaataagtga tgcatttgat ttagtaaaat
    40381 tatcacaaga atttattgat gaagtaaaac caaaaattga aaacaactac cctgaaattg
    40441 caccatttgt aggtaaatta aagggtgatg atatagaaga aggtaaagaa ttagtagcaa
    40501 cacacatagc tgaaggatta taccatgatg gaaaattcgt tggttatgta aaaagagcac
    40561 atgatgtaga tgttaactta aatgcccata caatgtttga aaatctagta gtaaaagcat
    40621 ctggagtttt atctgctatt caaatgctaa gacatagtaa aatagaccct gctgaaatag
    40681 attatgttat tgagtgttca gaagaagctt gtggagatat aaaccaaaga ggtggaggaa
    40741 actttgctaa atcaatagct gagatagtag gacttcaaaa cgcaactggt tctgacacaa
    40801 gaggattttg tgcagcacca actcatgcat taattcaagc agcagcttta gtaaaagctg
    40861 gtatacataa aaatgtaatg gtagtagcag gtggagcaag tgctaagctt ggaatgaatg
    40921 ctaaagacca tgttaaaaaa ggtcttcctg tacttgaaga tgttgttgga ggatttgcag
    40981 tacttgtatc tgaaaatgat ggtgtaaatc cagttataag aactgattta acaggtaagc
    41041 atactgttgg aactggttct tcaccacaag cagttatgac agcacttata acttcaggtc
    41101 ttgatagagc taatttaaaa ataacagatg ttgatgtata ttcagttgaa atgcaaaatc
    41161 cagatataac taagccagca ggagcaggag atgttccaga agcaaactac aaaatgatag
    41221 gagcacttgc tgttaaacgt ggagacttag aaaagaaaga attgaaagat tttgtttcta
    41281 acaaaggatt accaggatgg gcaccaactc aaggacatat accatcagga gctccttata
    41341 taggattttt aatagatgat ttaactacag gaaatagaaa tagagctatg atagttggaa
    41401 aaggtagttt attcttagga agaatgacta acttatttga tggagtatct tttatagctg
    41461 aaagaaatac aggtgttact gaagagactt caggaatatc taaggatgaa attaagaaga
    41521 taatagctga atctatgaaa aaactagcat tagatatgtt agaagaatag gggtgaggat
    41581 atgtcaaaaa aagtaatcgc agatgtattc ttagaagtag ctaatgcaat agaaagcggc
    41641 gaatttggca aaaaagtaaa aattggtgtt actacacttg gaagtgaaca tggtgtagag
    41701 aacatggtca atggtgcaca attagcaaaa tctaatttat ttgatattgt gcttattgga
    41761 ccaaaagtgg aaactgacct tgaagtagtt gaagttaatg atgaaaaaga aatgcatgca
    41821 aaaatggaag aattattaga ttcaggatat attgatgctt gtgttactat gcattataat
    41881 tttccaatag gtgtatctac agtaggtaga gtaataactc cagcaaaagg aaaagaaatg
    41941 atacttgcaa caacaacagg aacaagtgct actaatagaa tagaggcaat ggtgagaaat
    42001 gccatttatg gtattgctac agcgaaatca atgggtaaca aatgcccaaa agttggaata
    42061 cttaatgttg atggagcaag acaagttgag aagtgcttaa aagaattaaa agataatggg
    42121 tatgatatgg aatttgcgga ttcaattaga gcagatggtg gatgtgtaat gagaggtaat
    42181 gatttacttg taggtgcacc tgatgttatg gttactgata cattatctgg aaatatattt
    42241 atgaaagtat tttcttcata tactacaggt ggagattatg aagctcaagg atttggatat
    42301 ggacctggag ttggagagga ctatgataga aaggtactta tagtatcaag agcatcaggt
    42361 tctccggtag tagcaaatgc tttaaaatac gcttatgatg tcgtaaaggg agatataagt
    42421 aatgttgcaa gaaatgaatt tgcaaaggtt aaaaaagcaa aatttgctga cattatatcc
    42481 tctcttacta aaaaagaagt taaagctgaa aaagtagaag ttaaaatgcc agataaagag
    42541 attgttactc gtcaaatagc tggtgttgat ataatggatt tagaagatgc agtgtctgag
    42601 ttatggaaaa acggaatcta tgctgaaagt ggtatgggtt gtacaggtcc aatagtttta
    42661 gtaaatgaag ctaaaggaga cttagcagtt gaaactttag ttaaagctgg atatactgca
    42721 aaataagtac actttttatg taaataagaa catatactta taatcaataa aattataagt
    42781 atatgttctt tttattttct actttgtcaa tgtttatata ttaacataat ctttttattt
    42841 gattttctag ctaatttatc atatttgttc aattgaattt aagtttttag ctaaataaaa
    42901 ctaataaaat attaaagtaa aattaataaa atctgaaaaa attttagaaa taaactatct
    42961 attagataaa aagaaggcta attagtgata taatataatt aggataaaat ttattatttg
    43021 gaggcaaaat catgagcaaa gtaaaaaaag tagtagaatt gttggaatct aaaggtattg
    43081 atgcactata tctaacaaag aaaactaatg taaactatat aagtggtttt cctgatgaag
    43141 aagcatatgc agtgatttgt aaatatggaa actttttagt tactgatagt agatatatgg
    43201 agcttgcgga aaatgtttgc gaaggttttg aaattataaa ttggcataat tttgatagaa
    43261 gtgttgcaaa ggcagttaaa agtgtatgtg ataaagtggg aataaagaaa cttggatttg
    43321 aaagaactaa tatagtgttc gataaatatg aagaacttaa aaatctgatt gagaaggata
    43381 atggagaatt aatacctacg gaaaatattg tagagacttt gagatatata aaagacgaag
    43441 aagaaatcaa aaatacaaga aaagcttgtg aaatagcaga taaagcatta gaagaattga
    43501 ttccacatat aaaagctgga gttagcgaaa ttgaactggc tacgaagtta gagtacttta
    43561 tgaagatgaa tggagcacaa aatataggat ttgaaactat attgatatca ggagcaaaga
    43621 cttcattgct tcatggtaag cctagtgata agattataga aaaaggtgat tttgtactta
    43681 tagactatgg tgctatgtat aatggttata tatctgacac aactagaact tttatagttg
    43741 gggaagcttc tgaaaagcaa ttagaaatat ataatttagt taaggaagcc caaaatgtag
    43801 gggttgaaaa catgaaagtt ggtgtacatg cgacaattcc agatgctgag ataagaaaag
    43861 ttgtcaaaaa atacgaagac tattactatc agggtatagg acatggagtc ggtagagatg
    43921 ttcatgaaga acctttcatt ggtaattatg gtgataaaat catagaagat ggatgtataa
    43981 taacaatgga accaggtatt tatttccctg gatggggtgg agttagaata gaagacactg
    44041 ttttgattac taaaaatgga ccagaaagac ttactaaatt ccctaaagat ttgatgattt
    44101 tagacaaata atttaattta tatatatttt tacataaaat ataacataat ctcactatta
    44161 agccctattt acatattatg ataaagaggg cttaatttta ttgtgagagg tgataatgtc
    44221 catgtataaa aaacataaat ttataaaaaa tataaaacaa gcctggtata aaatcaaact
    44281 aattgataga tgccttataa ttgtaatggc aatattaatg tttcaatcta tatacaattt
    44341 atttgttaat gaagtaaatt cacaagacac tacaactatt gatgtagtag taagaactac
    44401 ttcagcagct atatttggat actttttaag tgttaatttt ataaagagac cttcaagaaa
    44461 gtatataaat actgatgtat cttcaaattt ttttatagat aataatttat ctaagaaaag
    44521 tcaagaatca agtcagaata atgtaatgaa tgtcaaaaat accattggat ttacttcaga
    44581 aactgataat tatgaaaaga aaatacctac taataatact gaagattttg aagatggaga
    44641 gactagtgaa ttacaaattg ttatagctac tgtaatttgt gtagtagcat taacagtact
    44701 ttttgttgta agaaatttta caactacaac atcagcttct ttaggtacta tttcacagat
    44761 gagagatttt gtctcaggat gcgtgggttt tttactaggt tgtccaagta gacctgatta
    44821 aaaatgtgat tactaataaa aaagattgtt aaaagtatca gtataataat acttagacat
    44881 taaggcatat aattataatc ttataagatt tgatatccta tttttatact gatactctaa
    44941 aatatttatt aaaacatttt taatattaca tttaaataaa atattatggc tacataaaag
    45001 ttaatttata tgaatagatg ccatatattc aataaaaagt ttcatactat gagatatgta
    45061 ttgatttctt ctccaaagaa ccccaatatt tgcagtaatt gcacgctcta atggtattga
    45121 acagagattt tttgtatttt gaaatatttc tttatatagt attgttgaag ccaagtcatt
    45181 ttctagtaaa taacgtatgg tagaaagttg gtttgagtgt aaaattatat ttggcttagc
    45241 tttttcaagt ctttttgtaa taatcttata gtgataagaa ccttcagata agattacaaa
    45301 aggtacattt tttaacatat aacttgatat aatggggtgt tttgccaatt catttttggg
    45361 atttgtaact aaatataatt ctgtcttata gattgtcttt gaatctatat catcataaat
    45421 atcactatca attataccaa tacaaaaatc aagtgtagat tcttcaatca ttttagctcc
    45481 aacaaatgtt ggaacttcaa aaattttaag ctctatatct ggataaatta cattaaatct
    45541 aggaagtatt ttcgaaagta aaaatgtacc taaaactgta ggaataccta ttttgagaga
    45601 agcttttctc ttttttccca aatccagtgc gtcacattga aaatcctcaa attgttttat
    45661 aaaatgtttt gacttgtcta aaaagatttt tccttcaggc gttaactcta ttttattacc
    45721 tattctataa aaaagttcaa agccaagttc tttttctaat tctttaattg ctaatgttat
    45781 ggatggttga gaaatgtgaa tactttccgc agccttagtc atgtttttat atttacagac
    45841 agtttgaaaa tagtataaat agtttatatt catttggtat tacttccttt catttacgta
    45901 ttagtgacaa gtatataatt aacaatagta taagttttgt atattgttgt aaaaaaatag
    45961 aatagtttta aactatcata tcataagttt ttgatatttt caatctattg agaatagtgt
    46021 tattattaaa ttaacaaaag agaatagctg atatggttta ctctaaatca atctataata
    46081 cagttatatc aacgaatatt aaaaaaatat ctattaaaaa ataaaaaata tttttacaat
    46141 taaattgtta attaaatcgt aaagaaaaaa atgatattat caagataaaa tttttttaat
    46201 tagaagtgtt aatttaataa caaaatagag tcttagggag aaaggagaaa tatatttttt
    46261 aataatattt gttaatttaa taacaaaaaa tgaaagggag tgatttggtg aagtttgaat
    46321 tgatggattt tttaatgaat ccttttgtcc ttatgtttgc tgcagtaatt acaggaattt
    46381 tatttggaaa aattaaattt gggaagttta attttggggt atcaggagca ttatttacag
    46441 gattatttat tggatggctt gcatatagct tgggaaattt aataatagca aaaggagaaa
    46501 cagcagcagg atataaggca gcaactgtaa tgatggggaa tggaattatt tctcctgact
    46561 tctttgattt ctttttaata atatttgttg cagcagttgg tctattggca gcaaaagata
    46621 tgaaagctgt attaaagaag tatggagcaa gatttgtaat tttaggcgtt cttataactt
    46681 tcataggtgg ctctatgact tatgcaatga ctttattatc aagtgataaa ggaagtagcg
    46741 cttatgaagt ttctggtgtt tacacaggag cattaacaag ttcacctgga ttagccgctg
    46801 ctttagaaac agctggtaaa catgcagaag atgtatcaaa agaatttgaa aaagcttcaa
    46861 caaaagataa agaagaaatt ttaaaagtag tcgacccaga aggaaaatta gatgtgagca
    46921 caactacatc actgactcaa gagcaaatag ataagtacat tgcatatgct gaagctggtg
    46981 ttggtatagg tcatgcagtc gcatatcctt ttggagtttt aatagtaatc ttaggtgtaa
    47041 atttccttcc aaaattattt aggatggacc ttaaagaaga aagaagaaaa tatgagaagg
    47101 aaatgaaaga agcaagagat agtgtttctg gtaaaacgat accagaagtc ccatttaata
    47161 taataacatt tttcttgaca tgtttagctg gatacttagt tggaggaata catgtattta
    47221 tgggaccact agggtacttt acactaggag caacaggagg ttcactaatt gtatctctag
    47281 ttcttggata tataggtaag ataggggtaa taaattttag aatggaagaa aaggttttaa
    47341 atattttaaa acaaataggg ctagtatttt tcttagcaat agttggatta agatatggtg
    47401 gaaaagtagt tgattcaatt atgacatctg gtatgcacct agctttagta gcaatagcag
    47461 tgggtgttac agctatgatg ataggattct tagttggtaa atatgtattt aaattaaatt
    47521 ggattttact atctggagct gtctgtggtg gtatgacatc aacaccaggc cttggagctg
    47581 ctgttgatgc tcttgatagt gatgacccag cagcaggtta tggtgctacg tatccatttg
    47641 cattattaac taaagttata ttggttatag ttttacacaa acttccaatg taaataagat
    47701 tttatatggg gggatattta tgaataatga aggaacattt gaagaactaa gtggaagatt
    47761 aagaaataaa aaattgtgtg atttaataat gaatgcagat gaagctgcta aatttataaa
    47821 ggatgggatg gttgttggag ttagtggatt tacaccttca ggttatccaa aagcagtacc
    47881 attagcagtt tcagaaagag ctaaaagtgg agaaaagata aaattaactg tatattcagg
    47941 agcatcttta ggtccagaag ttgatggagc atggtcagaa gcaggtataa ttgaaagaag
    48001 acttccttat caaactaatt caatacttag aaataatatt aataaaggtg ttgttgatta
    48061 tatagatatg cacctaagtc attctactca atttttaaat tatggaacta tacccaaagt
    48121 agatgtagct atagtagaag cacttgcaat aactgaagat gggaatatta taccaactag
    48181 tggaataggt aattctccat cttttataaa aagtgcagat aaagtaatag tagagataaa
    48241 tcttgcaaaa ccaatggaaa tggaaggtat ggcagatata tatattacgg aaaatccacc
    48301 aaatagaaaa ccaatagaaa taaatcatcc taaagataga attggaacaa catatatacc
    48361 ttgtggtttg gataaaattg caggaatagt aattactaat atgcaagata aaacccgacc
    48421 attgggtgta gtagatgaag cttcgaaaaa aatatcaaat aatattattg cttttttaag
    48481 agaagaagta aaggcaggta gactgtcaaa gaatttactt cctcttcaat ctggtgtagg
    48541 aagtgtagca aatgcagtat tatatggatt atgtgaatct gaatttgaaa atttaacttg
    48601 ttacacagag gttgtacagg attctatgtt ggatttaata agaatgggca aagtcacaat
    48661 ggcatctact acatctgtaa gtccatctcc ggaaggatta ataaaatttg aaaaagatat
    48721 tgattttttt aaggataaga ttatattaag acctcaagaa attagtaata atccagagat
    48781 agctagaaga attggtgtaa ttgcaatgaa cactgcaatt gaagttgata tttatggaaa
    48841 tgtaaattcc actcatataa tggggtcaaa gatgatgaat ggaattggtg gttctggtga
    48901 ttttgctaga aatggagcaa taactatatt tagtactgag tcaattgcaa aaaatggtga
    48961 tatatcatca atagtaccta tggtatctca tgtagaccac actgaacatg atgtaatggt
    49021 aattgtaaca gaacaaggat atgctgattt aagaggtctt gcacctagag aaagagctat
    49081 aaaaataata gaaaattgtg cccatccaga ttataaagaa caacttagag attatttaaa
    49141 tagagcttgt aaaagtggag caaaacaaac acctcacata ttggatgaag ctttatcatg
    49201 gcatagtaaa tttatgagta caggtacaat gaaaaaggct gaaactttta aaagtgcatt
    49261 ataaaatatt taataaattg gaggtagatt ttaaatggga aaagcagtgg aaaattttga
    49321 agatttaagt aaggaataca ttaatggtta tattgagaga gccagaaaag ctcaaagaga
    49381 gtttgaatgt tatactcaag aacaagtaga taaaatagta aaaatagttg ggaaagtggt
    49441 gtattataat gctgaatatt tagctaaact tgctgtagaa gagactggaa tgggagtata
    49501 tgaagataag gtagctaaaa ataaaagcaa agcaaaagtt atatataaca atttaaaaga
    49561 taaaaaatca gttggtataa ttgatataga tagagaaact ggaataacaa agatagctaa
    49621 accggttgga gtagtagcgg caataactcc ttgtacaaat ccaatcgtta cacctatgag
    49681 caatgcaatg tttgcgctta agggaagaaa tgcaattatt atcacacctc atcataaagc
    49741 aattggatgt agtacaaaaa ctgttgagat gattaacgaa gaattagaaa aaataggagc
    49801 accagaaaat ttaatacaaa tactggacca acaatcaaga gaaaacacaa gaaatttaat
    49861 ttcatcagca gatgtagtaa ttgccactgg cggaatggga atggtaaaag ctgcatactc
    49921 aagtggaaaa ccagcacttg gtgtgggagc aggaaatgtt caatgtatta tagatagaga
    49981 tgtagatatt aaagaagcag tacctaaaat catagcagga agaatttttg ataatgggat
    50041 catttgttca ggcgagcaat cagtaatagt agcagaagaa atgtttgata agataatgga
    50101 tgaatttaaa aataacaaag gttttatagt tagagataaa gcacaaaaag aagctttcag
    50161 aaatgctatg tttgtaaata aatctatgaa taaagatgca gtaggtcaat cagttcatac
    50221 aatcgcaaaa attgcaggtg ttgaaatacc agaggataca aaaataatag taatagaagc
    50281 tgatggacca ggagaagagg atattattgc aaaagaaaaa atgtgtccag ttatatctgc
    50341 atataaatat aaaagttttg aagaaggtgt agctattgca aaagcaaatt taaatgtaga
    50401 aggaaaaggg catagtgtat ctatacattc aaatacagtt aagaatatag aatatgcagg
    50461 agaaaatata gaagtatcaa gatttgttat taatcaatgt tgtgctacta gtgctggagg
    50521 aagtttcttt aatggtcttg cacctactaa tactctaggt tgtggttctt ggggaaataa
    50581 cagtatctct gaaaatcttg attataagca tctaattaat atttctagaa ttgcttatta
    50641 tatgccagaa aatgaagttc ctacagatga agaactttgg ggataaaatt tagacaaaaa
    50701 atattcattc taaattaaaa taagaatata ttttaattta gaatgaattt aagacataaa
    50761 ttaaatttaa attagtctat tagaccaaat ttaaaatatt aagagacata tagaagaagg
    50821 aagtattata atttaaaata aataaaaaat tctataaaag agaaggagtg gttttattat
    50881 ggcattaatg acaggagctc aatatattga aagtttgaga aaattaaata ctaaggttta
    50941 tatgtttggt gaagaggtta agaattgggt tgaccatcct atgattagac catctataaa
    51001 ttgtgtagca gcaacttatg acttagctca tgaccctgaa tatgcagatt taatgacagt
    51061 tacttcaaat atcacaggtg aaaaaataaa tagatttggt catcttcatc aaagtactga
    51121 tgatttgata aaaaaagtaa aaatgcaaag actatgtggt caaaaaacag cttcttgttt
    51181 ccaaagatgt gttggtatgg atgcatttaa tgcagtatat tcaacaacat ttgaatgtga
    51241 taaagcccat ggaactaatt atcatgataa ttttgtaaaa tacttaactt atatacaaga
    51301 aaacgattta gtagttgatg gagctatgac tgaccctaaa ggagatagaa gtttatcacc
    51361 tagtgctcaa cctgacccag atatgtttct tcatatagta gaaagaagag aagatgggat
    51421 aattgtaaga ggtgcaaagg cacatcaaac tggttcaata aattcacatg agcaccttat
    51481 aatgcctact ataagcatga ctgaagcaga taaagactac gctgtatcat ttgcagttcc
    51541 ttcagatgct gaaggagtat ttatgattta tggaagacaa tcttgtgaca ctagaaaatt
    51601 agaagaaggt gcggatgttg atttaggaaa taaggagttt ggaggtcagg aagctttagt
    51661 agtgtttgat aatgtatttg ttccaaatga tagaattttc ttatgtggag aatgggattt
    51721 ctctggtatg ttagtagaga gatttgcagg atatcataga caaagttatg gtggatgtaa
    51781 agtaggagta ggtgatgtaa taataggagc agcagcctta gcagctgatt ataatggagc
    51841 aaacaaagct tcacatataa aagataaact aatagaaatg actcacctta atgaaagctt
    51901 atattgctgt ggtatagcat gttcatcaga aggtcacaag acagaagctg gaaactatca
    51961 aatagattta cttcttgcaa atgtatgtaa acaaaatgta actagatttc cttatgaaat
    52021 agtaagactt gcagaagata tagctggagg gcttatggtt actatgcctt cagaaaaaga
    52081 ctttaaatca gaccttaaag ttggaacaag tggaatgact attggcgaag tatgtaacaa
    52141 gtattttaaa gcttcttcag tagcttcaac agaagaaaga atgagaatat taagattttt
    52201 agaaaatata tgtcttggtt catctgctgt tggttataga actgagtcaa tgcacggggc
    52261 aggttcacca caagctcaaa gaataatgat ttcaagacaa ggaaacataa atcaaaagaa
    52321 agaattagct aagacaattg caggaataaa aaaagaagaa gctttaaatt tataaaaagt
    52381 tgatatataa agggaaatta tatattaatg tttattaatt aatgaaaaaa tatggagtat
    52441 aagagataat tataaaatta tggtggtttt tagctaagta aagaaatttt ttataaggag
    52501 aatttacaca tgacctgtgg agttagattt tgtggcggct gtaaccctag atacgaaaga
    52561 gggaaggctc ttgcaataat caaagagcac ttcaatggta gagtagattt tagtatagca
    52621 caagagaact tggagtatga tttgctactt ataattggag gatgcgcaac tctatgcgca
    52681 tcctatcaac aatataaagt gaaaaatgaa tgtgttaaga tgtgggattt tagccatata
    52741 gaaagagtta taaatgtgat tgaatcagca atttgtgaaa cttaaaaaag ctaaatgggg
    52801 ggatttcatt gagttggcga gaattgtatc aaagtaagtt atgtagtgct acagaagctg
    52861 taaaacagat taaaaacgga gatactgtag tatttgcaca ttgtgtaggt gaacctcctg
    52921 cactagtaga ggctatgatt gaaaatgctg aacaatataa agatgttgag attaaacata
    52981 tggtaagctt gggtagtggt ggatatactg ctaagggaat ggaagctcat tttagagtaa
    53041 atccaatgtt tgtaagtgga aatgtaagaa aggcaattga aaacggagat ggtgatttta
    53101 cgcctacatt cttccatgaa gtaccaaagt tgcttagaga aaaaagatta aaatgtgatg
    53161 ttgttttagc acaggtaact ccacctgatg aacacggata ttgtagctta gggacatcag
    53221 ttgattatac ttatgaagct attaaaactg ctagaacagt aattgttcaa gtgaatgacc
    53281 agtttcctag aacttatggt gaggtagtcc atgtatcaga ttttgactat atagtggaaa
    53341 aatcacaacc tttatatgaa ttagaacctg caaagattgg agaagtagaa gaagcaattg
    53401 gtaaaaactg tgcttcatta attgaagatg gttctacttt gcaactaggg attggtggga
    53461 taccagatgc agttatgtta tttttaacag ataaaaagga tttaggaatc cattcagaaa
    53521 tgatttcaga tgggactttg gctctttatg aaaaaggtgt tataaatggt aaatataaaa
    53581 attttgataa agagaaaatg acagttacgt tcttaatggg tactaaaaaa ttatatgact
    53641 ttgtaaataa taaccctgcg gtagaggtaa aacctgtaga ctatgtaaat catccagcaa
    53701 ttataatgaa acaacataag atggtttcca taaattctgc tatacaagtt gatttgatgg
    53761 gacaggtagt tgcagaggct atgggactta gacaatttag tggtgttgga ggtcaagttg
    53821 actttatacg tggagtgtct atgggagaag atggaaaagc aattatagct atgccttcaa
    53881 taactacaaa aaaagatggt acagtaattt caaaaattgt atctattgtg gatgaaggtg
    53941 cacctataac aacatcaaga aatgatgttg actatattgt aacagaatat ggtatagcag
    54001 aattaaaagg aaaatctctt agagaaagag ctagaaattt aataaatata gctcatccaa
    54061 gtgtaagaga aagtctagca atagagtttg agaagagatt taaggaaaaa tattaataat
    54121 agagaggtgt taataatgga agcacttaga gtagttccaa agatatttta ttttgataca
    54181 tttaaagaat ttaatgaaga atttaaaata ggaaaaaatg atttagtaat tactaatgag
    54241 tttatatatg agccatatat gaaacctctt ggaatagata caaatttaat ttttcaagaa
    54301 aagtttggaa caggtgaacc atctgatgaa atgatagatt ccatgacaaa ggaaatgaag
    54361 aaatacaatt ttgatagaat tattgcgttt ggaggaggaa caatagttga tatatgtaag
    54421 atattagcgc ttgatgttcc agaaaagtct attgatttat ttgaaggaga agtaccacct
    54481 aaaaaaataa aagagttagt agtagttcct acaacatgtg gtacaggtag tgaagtaaca
    54541 aatgtagcta ttgcagaatt aaaatctaaa catactaaaa aggggcttgc agtagaagaa
    54601 acatatgcag actatgcagt gctaattcca gagacagtaa aaggtttacc ttataaattc
    54661 tttgttacaa gctctgtgga tgctcttata catgctattg aatcttatct ttctccaaaa
    54721 gcaagtccat ttacagaaat gtattctctg caagcaataa aaatgattat ggatgggtat
    54781 aaaaagatag ttgataaagg agaagaagag agatttaatc atcttagaga ctttgtcctt
    54841 gcatcaaatt atgcaggaat tgcttttgga aatgctggtt gtgcagcagt tcatgcacta
    54901 tcttattcaa taggtggagc tttccatgtt gcacatggtg aagcaaatta tcagttcttt
    54961 actgaagtat ttaagatgta ttctagaaaa aaacctaatg gaaaaattat aaaatgtaca
    55021 aaaattttag cagatgccct tgaatgtgac cctaattgtg atgtttatgg agaattagaa
    55081 aagtttttaa acaagttaat agctaaaaaa gctttgagag aatatggaat ggtggaagct
    55141 cagatagatg aatttacaga tagtacaata gcaaatcaac aacgattact tgctaataat
    55201 tatgtagaac ttaatagaga ggaaataaga gagatatttg caaatcttta ttaataaaat
    55261 tttttattta tatgtatagt caacataagc aatgttaatt atatttatgt acaaataaaa
    55321 acgagggaac ttaaatttaa ttaaagttaa gttctcttat ttttatatgg agttaaatgt
    55381 aagtatattt ttgatttaaa atgtacttac atttaagtct attactttat ttggaacaat
    55441 ttgtagaatt tcaaattcct tgacagtaat aagtaagtaa ttgtataatc aaactgcaat
    55501 atattaataa tattagtgaa aatgaaagga tataaaatat gtataaatca gaaaattttt
    55561 caaaaaaatc tgtattaata aattcatttt taagtaaatt tttaaaaata attggatggg
    55621 gtttagtaag tttatttaca ttatcagcat tagtactttc aactatttcc attataatcg
    55681 gatgggctgt aatacttcct atccaagtat gtgcagtatt tttaattata ggaggagttt
    55741 gtatttttaa aggtaagagg ttaggagacc aaatttctcg ttataaaaaa tattgtacaa
    55801 ttataaacaa tagaaatatt attccagtag aacttattgc agaaaataca tctaaatcat
    55861 taaattttat aataaaagat gtacaaaaga tgatagacaa aaattacttt ataaatactt
    55921 acattgataa aagaaataat cagattgtac tgacaaatga agattttgtt ccaacaagat
    55981 atgaagatgt agcatatgaa gttaaagaag aagaaaagac tgatgaagac aatgaagttg
    56041 atacaattat aaataaagga atgaattatc taacacatat aaaagaagct aataaaaata
    56101 ttaaatcaga atcaatgtgc gataaaattg tacaagtaga agatgttaca agtaaaatat
    56161 ttgatgttgt aaaacatgac ccatcaaaac ttactcaaat acaaaaattt atggattatt
    56221 atcttccaac tactttgaag ctattaaatt catatcatac attggaagaa caaggcattg
    56281 atagagaaaa tataacaaca actatggaaa gtattgaaaa tacaatgaat acgattgtag
    56341 tagcttttga aaatcaattg gactacctgt ttgaagatga agcaattgat atttctacag
    56401 atattactgt tttagagaat atgttagttc aagaaggatt aacgagtggt aaattttaac
    56461 ttcaatagaa aaaatattct gttattttta aacaattaat tagtcataaa gtataaaata
    56521 tgtgttagat aagttaagca gtactttatg aaggaagagg agagaaagat ataatgaata
    56581 atttagatga tataccagtt atgccaactt tgacgcttga cccatttgga gaagaatcaa
    56641 acactagtga tatagataat tctgacttat taatgaaaaa ggatgaaaaa gacccagaag
    56701 aagaaaagct gtctgaatct gaacgtaaga tggtaaagga atttgcggaa aaaatagata
    56761 ttacaaatac aaatatgatt ttacaatatg gagctggtgc tcaaaaaaag gtggcgagct
    56821 tttctgaaac agctttaaag agtgtcaaaa caagagattt aggagaagtt ggagatatgc
    56881 ttacaaatct agttactgat ttaaaagcat tttcagcaga tgaaaaagaa caaagtggtt
    56941 ttttgggaat ttttaaaaag gcaaataata aaatttcaaa tctaaaaact aagtatgata
    57001 gtgcagaggt aaatgtagat aaagtaagca aagaacttca aaagcatcag gttaaaatat
    57061 tgaaagatat agcaatgctt gataagatgt atgagttgaa cttagcttat tcaaaagaac
    57121 ttactatgta tattatagcc ggaaaacaaa aactaaaaga tatgaaagaa tatgagatgc
    57181 caaagttgag agaaaaagct cgtttatctg gttcaacaga agatgctcaa tctgtaaatg
    57241 atatggtttc actttgtgac agatttgaaa agaagattca tgacttagaa ttaactagaa
    57301 tggtatcgct tcaaatggcg ccgcagataa gattggttca aaatagtaat aatctgatgg
    57361 cagaaaagat tcaatcaact atagtaaata ctattccatt atggaaaaat caaatagtac
    57421 ttgctcttgg aatttctcat ttaaatcaag ctatgaaagc acagcgtgaa gttagcaata
    57481 tgacaaatga gcttttatta aagaatgcag aaactttgaa gatgggaaca atagaaacag
    57541 caaaagaatc agaaagagga attgtagata tagatacaat taagaaaact aatcaatctt
    57601 taatatctac tattgatgag gtcgttaaga tacaacatga tggtagaata aagagacaag
    57661 aagcagaagt tgaattatca aaaatagaaa atgaacttaa aagtaagctt cttgaattta
    57721 cagttaaata aaaattatac taagataaaa aagagactat cccaaaatag agataagtct
    57781 ctttttcatt gtttaaatga ttaaagtata tacttatatt ataaatttct attatctttt
    57841 aacataacat catgagcccc accttcaact atactagtag aagatacaag cgtaagttta
    57901 gcattttttt gaagttcagg aatactaagt gctccacagt tacacatagt agaacgaact
    57961 ttacttaaag aaagagttac gttgtctttt aaagaaccag cataaggcac ataagaatca
    58021 acaccttctt caaaagaaag tttcttatct ccaccaaggt catatctttg ccagtttcta
    58081 gcacgagcag aaccttctcc ccaatattct ttcatgtaac taccatttat attaactttg
    58141 ttagttggac tctcatcaaa gcgagagaag tatctaccaa gcattataaa atctgcaccc
    58201 atagctaaag ctagagttat atgatggtca taaacgatac caccatcaga acaaattgga
    58261 atataaatac ctgttttttc aaagtattca tctctagctt gagcaacttc gattatagaa
    58321 gtagcttgtc cacgtcctat acctttttgt tcacgagtta tacatataga accaccacca
    58381 ataccaactt taacgaagtc agcaccagct tcagctaggt aaaggaatcc ttcacggtca
    58441 actacatttc cagctcccac tttaactgta tcaccatatt tttcacgaat gaaatcaaga
    58501 gttatttttt gccactcaga aaaaccttct gaagaatcta tacataaaac atcagcacca
    58561 gcttcaacta aagcaggaac acgttcagca aagtctctag tattaatacc agctccaact
    58621 acatatcttt tagaagaatc taaaagttca agtggatttt ctttatgaga agaataatct
    58681 ttacggaata ccatgtacat aagattacca ttatcatcaa gtacaggtag agagttaagt
    58741 ttgtggtccc atataatgtt gtttgcttct tttaaagtaa catctttgtt agcgtaaact
    58801 atagatgaca taggagtcat aaattcacta actttagtat ctaaatccat acgacttata
    58861 cgataatcgc gacttgcaac aatacctaat aatttaccag ttgaagttcc atcttcagta
    58921 acagctactg tagaatgtcc agttttttct tttaagtcaa gtatgtcttt caatgtgtta
    58981 tcaggtttaa tatttgaatc acttacaaca aaaccagctt tgtgggattt aacacgtgcc
    59041 accatagcag cttcattttc aatagtttga gaaccgtata taaatgaaat accaccttct
    59101 ttagcaagtg caatagccat tttgtcatca gaaacagatt gcataatagc agaagtcaaa
    59161 ggtatattca tataaatatc tgcttcctcg ccctttttga atttagtaac aggtgttttt
    59221 aaacttacat tagcaggtat acaatcagta gaagagaaac ctggtacaag aaggtattca
    59281 ctgaaagtat gtgatggtgt atcaaaataa aacgccattt tttaatcact ccttatattt
    59341 ttatactttt tataattgat agtattaatt ataacttatt ctttaatttt attaaagtat
    59401 tttgtcaaat attgagattt ttgaaaaatt taaactattt taattgaatt tgtttaatat
    59461 atattgcctt atagggtaaa gttgtatatt aaataatgtt tagtatttaa tagtagctaa
    59521 tttgaaatta atatttagtt attcatacat taaagttgta tattttatgt tgataatgtg
    59581 aacctagagt gtataaatgt caaaagtata gataaattaa atctatatta gtatatccaa
    59641 aagtaccctt aataaatttg tttaattgaa ttattatcgt tttttctatt aaatttaaat
    59701 attttataaa aatagtatta tttataaaac tagacaccct aaaatactac ataaataaaa
    59761 ttaaacaaaa actaaaatgg caaatgtcat tatctaaagt gacatagaaa cggtttctta
    59821 aagttgttac tataaaatat aaactttata ataaaagtat aacaaaaggg aatcatatcg
    59881 atatagaata taattcaaaa ggaggcaaaa ttatgagtag ttctaatcaa tcgacaataa
    59941 agacatctat acagcgagta gggaaatttt taagtggcat ggtaatgcca aatataggag
    60001 cttttatagc atgggggctt ataacagcat tatttatacc aacaggatgg atgcctaatg
    60061 aaaaattgag tactataggt gacccaatga taaaatacct tttaccacta ttgatagctt
    60121 atactggtgg taaagcaata gcaggacaaa gaggtggtgt aataggtgca gcagcagcta
    60181 tgggagtaat tgttggggca gatataccaa tgtttatcgg agcaatgata atgggaccat
    60241 ttgcaggatg gataattaag aagtttgata aatttgtaga tggaaaaata ccaacaggat
    60301 ttgaaatgct agtaaacaac ttttcaatag gtataatagg tatgcttctt gcaatactag
    60361 gattttatgc aattggacca gcaatagtag caggtacagc tcttatagaa tctggagttc
    60421 aatttatagt tagcaaaagt ttattaccgt tagtttcagt atttatagaa cctggaaaag
    60481 tactgttctt aaacaatgca ataaatcatg gtatattagg acctataggt atagctgagg
    60541 caaaagaagc aggtaaatct ataatgttct tattagaaag taaccctgga ccaggtttag
    60601 gagttctatt agcatattgg atgtttagta gaggaagtgt aaaacaatca gcaccaggag
    60661 ctgtaataat acatttcttt ggtggtatcc atgaaattta tttcccatat attttaatga
    60721 atccagtttt aatattggct acaatagcag gtggagcagc gggaatacta actttctcaa
    60781 tattaggagc tggacttgta gcagcaccat caccaggaag tatatttgca cttatggcac
    60841 tagctccaaa gggaggatta cttccagtat tagcaggagt tgcagtggca acagttgtat
    60901 cattcttagt ggcagcacca tttgttaaga gagcttcagc aaatcaaagt gaagaagaca
    60961 atacttcttt agaagaagct aaagcaaaag tgagtgatat gaaatctgta agtaagaact
    61021 ctaagaaaga tattgaagaa aaacagctgg aagtaaatga aattaagaag attgtatttg
    61081 catgtgatgc aggaatgggg tctagtgcaa tgggagcttc tagatttaaa aatagaataa
    61141 aaaatttaga tttaaatata gaaattacaa attcatctgt tgataatctt ccagatgata
    61201 cacaaatagt agtaactcat aatacattag ttgaaagagt tgctaagaac aattcaagtg
    61261 ttgagatagt ttcaataaac aattttttaa atgacccaaa tttagatgta ctatttaaaa
    61321 gactagaatc taaataaaat aaaaagtacc actaattagg agtggataat ttgaaaaaaa
    61381 agaagattac atctaggcaa aaaaagataa tactaatgat agtagaaaat tcaaagaaaa
    61441 atattccaat aactatttct gaaattgcag ggatgttgga gttaagttca agaactgtac
    61501 ttagagatat gtctggaatt gaaaaatggt ttgatgaaaa tgattttaac tttgtaaaaa
    61561 aaccaggagt aggattgatt ttagaggaaa gtatagaaaa tcaaaatttt ataattgaat
    61621 tattagaaga agaaaaaata gaaaaagaat acagcaagga agaaagaaat ttaatcatat
    61681 tatcaaagct actagtttct aacgagccag taaagtctta ttattttgca aaaatattaa
    61741 aggtatctga aggggtattg aataatgatt ttgctttagc aagtaaatgg ctggaaagat
    61801 ttgatattga gcttgttaga aaaccagggc tgggtgttta cttaaaaggt caagaaaaaa
    61861 gctttagaga agcatatgta aatcttatat atgattcatt taatgaaaaa gaaatactag
    61921 atatggtcag aaatataagt gaaaatatac aaacagataa aacaatagaa attttaagtg
    61981 aaaatagact tttaaattta atggataagt gcataataag aaaagttgaa ggtacattaa
    62041 caaagaaact ttcagattta gatgtaaatt tagctgatag tgcatatata ggtcttgtag
    62101 ttcatatttc attggcttta cagaggataa aaaatggtga aaatataact atggataaag
    62161 aatttctaaa agaattatct ataacagaag agtttaaatt agctaaagaa atcgtaaaag
    62221 gtatggaaat agattttagc atggatatac cactagatga ggttggatat ataactatgc
    62281 atataagagg tgcaaagcaa aggtcatctt caaatcacaa tgctttaaac ttagatgata
    62341 tagaagtaat ggatattaca aataagatga tagatttagc tgaagatgaa tttaaaataa
    62401 gtttaaaaaa tgacgagagg ttatttaaag acctagcaaa tcacttagga ccttctataa
    62461 atagattgaa tatgggatta gaaataagaa atcctctttt agatgaaata aaaagcaagt
    62521 attcatatgt ctataatgga gttgaaaaga tttctagaat tataaaagat aaactaagta
    62581 taaattcaat acctgaatca gaaataggat atattgcaat gcacttcgca tcagccatag
    62641 aaaaaaatct tatgatgtat gctaatataa atattgttgt ggcatgtccc actggaatag
    62701 gtacttcaag attcctatca actaagattg agaataaatt tcctaattta aatatacttg
    62761 aaaccatctc agctataaac atagatgaag agtatttaaa ggaaaaagat gttgatttaa
    62821 ttgtgtctac agttgagtta aatacaagtt taaattatat atgtgtaggt ccatttatga
    62881 gtttagatga cgaacagtta atcaaggaaa aaataaaatc tatagctcaa aataaattaa
    62941 tcaatttaaa tgtaaaaaat ggtactaaaa ataagaataa aatatatgca cagataacag
    63001 aaagtatgaa tataggaaaa gatattcttc aatttttaga ggaaattagg tttgaaaaat
    63061 ttgaaagtaa agatttaagt gagcttattg aagactcatc aaaaatattt gcaaaatcta
    63121 gtgaagatat aatatctata aaagaatctt taaaagaaag attaaaaatc tcaattccat
    63181 atatagaaga atcaaaaatt ctattattac attgtattag tgaaagaata gatattatga
    63241 agttatcaat aataaagcta gagaatagaa tcatactaga ttcaaatgaa gaaatcgaca
    63301 atgtagtttt tatgttactt cctaaaaatt caccaagtta tcaaagacaa ataatgagtg
    63361 aaataagtgg ttcattaata gataatataa tttttacgaa taagataaat aaattctcta
    63421 tagaagagat gatattagag gtaaaagata tagtttttaa tttttatacg aacagattaa
    63481 aagcatttat agataaataa aattaatatt gaaaaggaag gttgatacta tgaataaagt
    63541 attgaatgag aataatatat ttttagggtt agatagtgtt tcaaaggaag aagctataac
    63601 tttagcagga agaaagttag ttgaaaatgg atatgtaaaa gaagaatata taccagcaat
    63661 gctagaaaga gaaaaagtaa tgactacata tatgggaatg ggagttgcta ttccacatgg
    63721 tgtaaacgaa gcaaaaaaag aaatattatc ttctggaata gttatattac aattccctaa
    63781 tggtatagat tttgatggtg aaaaagcata tttattaata ggaatagctg gtgttggaga
    63841 tgaacattta gaaatactat ctaatatagc agtagtttta gatgatgact taacagaaag
    63901 actaaaaaat tcaaatgaca aacaagcttt tatggaagct tttgccaact aatcttagga
    63961 ggactttaaa atgaaaaagg caattcagtt tggagcagga aatattggaa gaggttttat
    64021 tggaggctta ttagttaagt cgggttatca tgtagtattt gctgatgtaa atgaggagat
    64081 attaaactca ataaacaaag ataaaaaata tactatacac ataagagatg tagaatgtat
    64141 agatgaagta attgataata taagtgcggt ttcatctata aaagaagaaa taatagatga
    64201 gattgtagag gctgaaataa taactacagc agttggacct ttagttttga ctaaaatagc
    64261 ttctacaatt gcaaaaggca taaagaatag aaaagaaaag ggattaacaa gtaatctaaa
    64321 tataatagct tgtgaaaatg caatatacgc aagctcttct ttaaaagaag aggtacttaa
    64381 gtatttaaat aaggaagaag tggaatattt agagatgtac attggttttc caaattgttc
    64441 agttgataga atagttcctc ctggtaaaaa tgaaaatcct cttgatgtaa cagttgaaaa
    64501 tttttatgaa tggaatgttg aaaagcaagg atttaaagga gaaataccaa ctatagttgg
    64561 tatgaattta gctgataatc ttatggctta tatagaacgt aagttgttta cactaaatac
    64621 aggtcatgct ataacagcgt atataggata tttaaaagga tataagacta tagaagaaag
    64681 tataaaagat aaatttatct atgacatagt aaaaagtgca atggtagaaa gcggagaagg
    64741 attaattaaa aaatacaatt ttgactcaga agcacactat aaatatattg ataaaatttt
    64801 aaatagattt aaaaatccat atcttaatga tgatgtatta agagttggaa gggagccatt
    64861 aagaaaatta agtgataaag atagattgat taaaccttta atgacagcaa aatcatatga
    64921 cctatcagtt gacaatttga ttcttggaat aggtgcagcg cttcattata ataatagtga
    64981 agatgctcaa agtgtagagt tacaagagtt gataaagtct aatggggtaa aaaaagctgt
    65041 tgctaaaata gcgaatataa ataatgatga agaactttta aataatatcg aaaaaagtta
    65101 tatatttatg gaaaatttat aaatttgctt gaaaaaagtt agttctgata aagtaagcat
    65161 aacatattat tctacacagt ttgaattatt tgttgaataa tagagaatta taatatataa
    65221 ttaaaaagaa taaattaata tatttacctg tatatgtttg ataatatggt tcaaatgttt
    65281 ctactaagct accattaata gcttgactac aggagtgaag aagatatagt ttatacttat
    65341 tttaaattat atattaagtt aattactttg aattttaagc tcctgtctat gatatagaca
    65401 ggagtttttt tattatctta atataaaata cttacaagaa tagatagcaa agttagagca
    65461 aatataattt ttttattaat ataactttag aaaggattgg gtggaagttt aatgaaggct
    65521 ttacaagaaa agatattgag agaaggcagt gtttcaggaa atgatatact aaaagttgat
    65581 agctttttaa atcaccaaat tgatgtagct tttttaaatg aaataggaag agagtttaaa
    65641 gagagatttg aaggagaaaa agttgataag atatttacaa tagaggcttc aggaatagct
    65701 atagcatcaa ttgtttcaca gtattttgat aatgcacctg tagtatttgc aaaaaaatcg
    65761 gaatctaaaa atttggataa agatgtatat gaaactaatg tatattcatt tactaaggca
    65821 agagaatatt ctgttaaagt ttctaaaaag tacataaaca aaggtgaaaa tatactgata
    65881 gtggatgatt tcttggcaaa tggaagagct gctcttggac ttaaagattt aattgagcaa
    65941 gcagaggcta acttagttgg agtaggaatt gtcattgaaa aaggattcca agctggagga
    66001 gcacttttaa aggctaataa tgtaagatta gagtctttgg cagtagttga atctatagat
    66061 aatggaactg taaaatttag gtaatttatg atattatata cttgtgttta gcacatattt
    66121 taactatgta attatggagg attttttatg agatttttta tagacacagc caatattgag
    66181 gaaataaaag aagcaaatga tttaggtgta atatgtggtg taactaccaa tccatctcta
    66241 atagctaagg aaggtagaga ttttatagaa gtagttaaag aaataagtga aatagtagat
    66301 ggaccaataa gtgcagaagt aataagttta gaacataaag gtatgataga ggaagcagaa
    66361 aaattatcta agatacataa aaatatagtt ataaagatac ctatgacagc agaaggtcta
    66421 aaagcagtga aagtactatc aagtaaaggt ataaagacta atgtaacact tatattctct
    66481 gcgggacaag ctcttttagc tgctagagct ggagctacat atgtaagtcc ttttgttggt
    66541 agattggatg atatatcaca aaatggtctt gatttaattg aagaaatcgt tgacatattt
    66601 agtgtaaata gcattgaggc tcaaataata gtcgctagtg ttagaaatcc aatacatgtt
    66661 ttacaagctg ctagaatggg agcagatata gcaacagttc ctcttaaagt tataaaccaa
    66721 atgattaagc atcctttgac agacaaagga atagatagct ttatgaagga ttgggaaggt
    66781 gcttctttaa aattgtaatt ataaattacg ttttataagt atattaaaga ggttattatg
    66841 gaaattctaa gtaataaggc taggctattt tgcctagtct tattttggtg tataatatat
    66901 tgaaacatgt tattgaaata gattaatttt ttaagtgagg aggcgaaaaa tggataaaat
    66961 attaagagag atacaaagag aagataaaaa aaatccatat actgaccaag aacttgctga
    67021 aatgttaaat gtagctaggt ctgaagtaat atcagttagg aaaaaaaata atattttaga
    67081 ttcaagagaa agaagaaaaa aaatattaat aaaagatata tcaaaaattg taagtgaaaa
    67141 tcctcaaatg tctgaaagaa aaataactga aatattaata gaaaatggat ataatatatc
    67201 gagaagtgca gtgtcaaaat tattaaaaga ggaaaattta aatcagtcaa agagtgggat
    67261 taaagataaa gacctagcat catcaggaaa gtatgtaaag gtgagtaaag ttgtagaaga
    67321 agatggattt gaagaattga taggtataaa gggaagcttg aaagaaaagg taaatctagc
    67381 taagtcagcg ataatgtatc ctcccaatgg ccttcatact ataatatatg gagaaacagg
    67441 agtaggtaaa agtgaattag ctacatgcat gtataaatat gctataaaaa acaacatcaa
    67501 agaagagaac agtccattta tagtgtttaa ctgtgcagat tatgcagaaa atcctaacct
    67561 attgatagct caactctttg gagtagtaaa aggtgcatat acaggagctg atgcaaatag
    67621 agagggtctt gtagaacaag ctaataatgg aatattgttt ttagatgaaa ttcatagatt
    67681 gcctgctgct gggcaagaaa tcttattttc attaatagat agaggagaat ttagaagatt
    67741 aggagaaagt agttctaata gaaaggcaaa tgttctcata ataagcgcta caacagaaaa
    67801 tccagattct aacttacttc aaacatttag aagacgtata ccaatggtaa taaatttacc
    67861 caatatgagt gaaagaccaa agtctgaaag atatgaaata ataataaagt tttttgaaag
    67921 agaagcaaag cgagtaaaca agaattttat aataactaaa gaagttatgt gtgcacttat
    67981 gaattataag tgtactggaa atataggtca gttaaaaagt gatatacaag taacttgtgc
    68041 tagagctttt agtaaatcga tattttctag cgataaggta attatagatt tagatagttt
    68101 aagggattat ataaaaagtg gatattatga ttgtaattgt gaaaatgaaa agtattctga
    68161 gttttcagtt gaagatatat atatagatat tagtgaattg gtgagtggaa aaaatagaat
    68221 tttatccgat aatgagataa gtgagatata taattatgca gaaaaagagc ttaaggtttt
    68281 ggaaagtaag tgcttttcag aagaagaatt aaaaaatgca tttatagaaa agttagacaa
    68341 gaagtttaat gctattaaaa acaataaaaa tttatctgat agaagaagtc aaatagactg
    68401 gggaggacaa ttaaaagagt ctacattgga gataatggat aaggtaattg tatttatcaa
    68461 ggagcagttt aagcatgtaa atcaaggctt gtatttggct ctagcaattc acattgagca
    68521 tgctatagga aggataaaag atggaaaaac tataataaat ccgagtttag ataaaataaa
    68581 agcatcaatg ccaatggaat atgagctatc gagatatata atgggtatag ttgaagattt
    68641 gacacaagta acatttccag aagatgagct tggatatttg gcttattata taaataaatt
    68701 ttgttataat gaggaaagta taaaagataa agttaaagta gtaatagtta cgcatggtaa
    68761 agttggaatt gaaatgtcta aagttgtaaa ccatatttta ggtatagaat gtacaatagg
    68821 tatagaaata gcacttacag attcaccatc agaggggata gaacatgtat tagaagagct
    68881 tcaaaagata gaggctcgaa aaggtattct agttctaatt gatatgggtt ctttggtaat
    68941 tcttggtgat gaagttgaaa aaagacttgg aattagatgt aaaactgtaa atagagtaga
    69001 tactttactg gcaatggaag caggaaaact tgcaactatt gaaggaaaaa gtttagatgg
    69061 tataatagca gatttaaaga aaaataagaa ttatgctatg ataaatacaa ataaatttag
    69121 ttacagaaaa aacgagtatg gaaagaaaaa tgtaatcatt actttatgct tgagtggtgt
    69181 aggtacagct ttaaatttaa aagatcatat agaaaaacaa attaaggaat atgatgcttc
    69241 aattgaagta aagccaattg cttttttaaa taataatttg gaagacgagt taaatgatat
    69301 tgaagctgaa tataatatta ttgctatctg tggaactatt gatatagatt ataaaaacgt
    69361 gccttttatt tcttataatg aggtgcttgg taaaaatggt attaataaag tattagagca
    69421 tttaaatagt aaacagccag ctttaaatat tgaaaatcag ttgaataatt taatacatga
    69481 agatttgatt ttatacaatt ttgaaggaat ttcaaaggag tatataattg atactcttgt
    69541 ttcaaagctt gaagaaggtg gatatgttga ttcaaagtat atattaagtg tatataaaag
    69601 agaagcaatg ggaagtgtcg taatggcctc taaggtagct gtgccacatg gacttccaga
    69661 aaacgttata aagcctgcaa tagcaattgc aaggttgaat aaacctatag tatgggacaa
    69721 taaatttatg gtggatttgg tggtactatt ggcacttaaa gaaaataata agaaagaaat
    69781 acgaagctta ttttcaaaaa ttaatgacca gcatacttta gaagttttat taaatactga
    69841 agataaaaat gaaataaaag aacttttaag ctaattatta gtacagagaa gaaagacaaa
    69901 aaatttgctt tagtataaaa tttaaaacta cttaattgct aagtagttaa ttactcgaga
    69961 taaggctagt tctatgaaat ttatttatat gattttatag aattagcttt tttcttggta
    70021 aataaaatat atgaatttat ttatgcaaaa agtaaatttt attgg
//
LOCUS       DAMJVM010000008        69238 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.008, whole genome shotgun sequence.
ACCESSION   DAMJVM010000008 DAMJVM010000000
VERSION     DAMJVM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 69238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69238
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.008"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(282..2240)
                     /locus_tag="PC523_000604"
     CDS             complement(282..2240)
                     /locus_tag="PC523_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HDF2474167.1"
                     /translation="MKRAYYSSSVNEFIGKDIFTIFGEITCNDQFEANDLQKNTWKKE
                     IEILKRELSTFKDGHLLFEYTIPRIGSRIDNVLIYKGIVFLLEFKVDEDTYPRYAIEQ
                     VTDYALDLNCFHKESHDKLLVPILICTKAPQRNQRIRLMKENILETYCSNEFNIGKYI
                     EEVCTTYNKSSFNPDAWMDSLYMPTPTIIEAAQALYMGHNVEDISRNDASAKNLNQTT
                     KAINKIIDYSKIHNKKSICFITGVPGAGKTLAGLNIAIERQKVDENEHAVFLSGNGPL
                     VDVLQEALARDDVTRNGTRKTEAIRKAKEFIQIIHHFRDEAISVDTPPIERVTIFDEA
                     QRAWDEPNLTNFMKRKKGVLDFNMSEPEFLISILNRHIGWATIVCLIGGGQEINKGES
                     DGISGWFASLRNNYSDWDVYISNKIIDEEYSKGNSFDELTEGVNYRIIDDLHLSVSLR
                     SFRSEKVAAFVKAVLDVDKIAANELYNQFKKDYPICVTRDLELAKKWVRDKSKGSQRY
                     GMTASSGAKRLRKYGVWVQSKVDAKNWFLNGKDDVRASYFLEETATEFDIQGLELDWT
                     IVCWDGNLRFKNEQFEYYNFNGTKWQNINKEDNILYLKNAYRVLLTRARQGFVIFVPT
                     GDESDITMQPSFYNGVYEYLKEIGIEEI"
     gene            complement(2922..3254)
                     /locus_tag="PC523_000605"
     CDS             complement(2922..3254)
                     /locus_tag="PC523_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HDF2474168.1"
                     /translation="MYVLLSKYFTQKELAKKQLLNYTALLSFEIDAKDVNDIKKFVNK
                     LDLFKIGVSWGGHESLVFAPNIAILNEMTLEQAKALNVSPYTIRISLGLENSEDLIND
                     LNDALSIL"
     gene            complement(3233..3717)
                     /locus_tag="PC523_000606"
                     /pseudo
     CDS             complement(3233..3717)
                     /locus_tag="PC523_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895448.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
     gene            complement(3698..4210)
                     /locus_tag="PC523_000607"
     CDS             complement(3698..4210)
                     /locus_tag="PC523_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2474169.1"
                     /translation="MKRLVAELTKEYLNKSMCYKEIMDCYIILIFYELLEIHKTKNEK
                     TLTSDSKINIDNIAEMMKYIEGNYAQTTLREVAKKFHFHPNYLSTLIKKTTGKSFKDI
                     IHEQRLKKATTLLKSTDMSIEDISIEVGYNNQTFFYKKFKETYSLTPKKYRIKSRILL
                     KEVSHETIYS"
     gene            complement(4294..4761)
                     /locus_tag="PC523_000608"
     CDS             complement(4294..4761)
                     /locus_tag="PC523_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HDF2474170.1"
                     /translation="MKDKTLEDILKQKTQAERYILECIDTNNVKKLDNKKIMSILHTI
                     LCWEEVNGTIVLTDKNILSLDEDIMVSKHHRFIDIPKHKHNFIELIYVYSGSCTQIID
                     GKETILKEGEICLLDMNVEHSINAVKENDIIINCIMRREYFDASFFLEFRLMS"
     gene            4947..5267
                     /gene="rhaM"
                     /locus_tag="PC523_000609"
     CDS             4947..5267
                     /gene="rhaM"
                     /locus_tag="PC523_000609"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDF2474171.1"
                     /translation="MNTVHKCFKMKLFKGFENEYKKRHDELWSEMIDMIHEYGGSNYS
                     IFLDEETNYLYGYIELKDAKLWDKSSETAICKKWWNYMSDIMETNEDNSPISIELKSM
                     FYLK"
     gene            6205..6780
                     /locus_tag="PC523_000610"
     CDS             6205..6780
                     /locus_tag="PC523_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2474172.1"
                     /translation="MSKIIAFVGSPRKNGNVDTIINEIIKGANKNKVDVKKYYLNDMN
                     IKGCQGCLYCRKNHSCSLKDDMIQVYEDIKKAEYVIIGSPVYICQVSAQTKLLLDRLY
                     PLTTIDKGKHIPRFGNKRIIMVYTQAAPLPIFFKRYFRYTSKHLKGMGLNHYKTIIAT
                     KAFEKNSIENNPKILKKAYNLGFKISKDINV"
     gene            complement(7450..7617)
                     /locus_tag="PC523_000611"
     CDS             complement(7450..7617)
                     /locus_tag="PC523_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474173.1"
                     /translation="MNWKELWMTLFGTTEWFGLNIGFWVAMAVVLLIVIVMNVVFWSM
                     KPKGDAKESYK"
     gene            complement(7630..10395)
                     /gene="mgtA"
                     /locus_tag="PC523_000612"
     CDS             complement(7630..10395)
                     /gene="mgtA"
                     /locus_tag="PC523_000612"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDF2474174.1"
                     /translation="MNKKENRMAVRQTVEKTVIRDEQNRRIQFAATNPIKEVLKKFHT
                     TPRGLEVDAIPVSRAKYGTNKVTHEKKKSLAKRLAGAFINPFTAILFCLAVVSTITDM
                     IFPYFSLFGSVPEDFDYLTVVIILTMVFISGTLRFVQESRSGNAAEKLLAMITTTCTV
                     TRRNQEKKEIPMDDLVVGDIVHLSAGDMIPADVRILDAKDLFVSQASLTGESEPIEKT
                     PNVSAQKESVTDYTSIAFMGSNVISGSATAVVVCVGDHTLFGSMASAVAGEAVETSFT
                     KGVNAVSWVLIRFMLIMVPLVFFVNGITKGDWLEAFLFGVSIAVGLTPEMLPMIVTTC
                     LAKGAVSMSKKQTIVKNLNSIQNFGAIDILCTDKTGTLTQDKVVLEYHLNVNGEDDTR
                     VLRHAYLNSYFQTGYKNLMDLAIIHKTEEEEATDSRLTDLSENYVKVDEIPFDFTRRR
                     LTTVVQDKGGKTQMVTKGAVEEMLSICAFAECNGEVQPLTDDIRYRILETVANLNDKG
                     FRVLAIAQKSNPSPIGAFGVKDECEMVLIGYLAFLDPPKESTADAIKALKDHGVITKI
                     LTGDNDKVTRTICKQVGLEVRNMLLGSDLEQMGDAELARVADSTDVFAKLTPDQKVRV
                     VSVLRENGHTVGFMGDGINDAAAMKAADIGISVDTAVDVAKESADIILLEKDLMVLEQ
                     GIIEGRKTYANMIKYIKMTASSNFGNMFSVLAASALLPFLPMMSVHLIILNLIYDLSC
                     TAIPWDNVDEEFIAKPRKWDASSVGSFMIWIGPTSSIFDFTTYIFMYFVFCPLFVSNG
                     VLFNDLPAHFSGAELAMLQAQYIGMFQAGWFVESMWSQTLVIHMIRTPKLPFLQSRAS
                     APVTLLTMTGITVLTIIPFTVFGTMLGFVALPATYFAYLIPCILLYMILATSLKKAYI
                     RHFGELL"
     gene            complement(10560..10871)
                     /locus_tag="PC523_000613"
     CDS             complement(10560..10871)
                     /locus_tag="PC523_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HDF2474175.1"
                     /translation="MDFTCIFFGILFTFAGFWLAVGKGYRHLSLWKNMPKEEKDKINI
                     VPLSRNVGGIIALNGIIFLMKGVLPGFSNHWFIFSIIAWLIVASFDVWYIEKSVRYRN
                     Q"
     gene            complement(11255..11758)
                     /locus_tag="PC523_000614"
     CDS             complement(11255..11758)
                     /locus_tag="PC523_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2474176.1"
                     /translation="MKKNISYIPMDSALEIWLLEKLLRDPPDSDYAKDLLSGIQEMIR
                     GEVSSLLLGSSEESDCPVGTLSDRILAGDLEIYPRGRKVLIKGSEVNLTPKEFDILYF
                     LVQNKGEVFTKEQIYRAVWEEDYLLDNSNIMAFIRKLRKKIEPAPDSPTYILTIWGIG
                     YKFNDQI"
     gene            complement(11774..14437)
                     /gene="mgtA"
                     /locus_tag="PC523_000615"
     CDS             complement(11774..14437)
                     /gene="mgtA"
                     /locus_tag="PC523_000615"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDF2474177.1"
                     /translation="MSKATLFDSRIKKYAYCEISEIYRDIGSSPDGLSIEQIGSMREK
                     YGANSFNGRKNDTTMQRLRRAFINPFHVILFVLGIVSLVTDVFLASNFARNATTAIII
                     FSMILISGVIRLIQELRAKSAAAQLDRLVHESVTVRRGGKLIEIPGEELVVGDIVLFS
                     AGDCVPADVRLTKITDLFISQAAITGESAILEKSCRKLSYGEQETLTQLENLAFMATT
                     VISGKGEGIVLAVGKDTLYGGFTKPDSDDKDSFKKGADSIAWVMIRFMAILVPIVFIL
                     LGITGGKWMESFAFALSVAVGLVPEMLPMVITACLAKGSLAMSKKQTIIKNINAMQSF
                     GSMDVLCMDKTGTLTNESILLEYYMDILGNENAEVLDLAYLNSSYHSGVCNTIDNAIL
                     ACNTMPGREAHYANLLTKFQKADEIPFDYARKFVSTLVQDSNGNSQLIMKGDIEHIFS
                     RCNYVEYHGKLLPMGQDARQSVSSVVGEMLQDGMKVIAVARKNVGAQKKITPDDEWGM
                     TLVGYLSFFDAPKQTAKESVTALKRLKVIPKILTGDQAAIAVSVCRRVGISTERILTS
                     TQLDEMTDCELKKAVEHTHVFAELTPGHKVRLVSAMQDNGHSVGFLGDGVNDIPALNE
                     ANVGISVDTAVDAAKDAADVVLLQKDLNVLEQGVMEGRKTFTNMLKYIKITASSNFGN
                     IFSIICASAFLPFLPMTSIQILLLNLLYDTLCIVLPWDNVDEEEMLSPRDWSGKTLRQ
                     FMLSFGPISSLFDIVTFLFLYYFLCPMLCGGATYLQLTDPSLKLQYVALFQTGWFLES
                     MWTQVLILHFLRTSKIPFFQSRASAPVISITLVGIIGFTYLTFAGGASLFGLTKLPLW
                     YFVFLLLVVFLYMLMNSVAKYFYKKKYQELI"
     gene            complement(14536..14745)
                     /locus_tag="PC523_000616"
     CDS             complement(14536..14745)
                     /locus_tag="PC523_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474178.1"
                     /translation="MVKVKTKKTFVCTHMCKVEIPMSVRKLYEKVLASSIRAPNLEGM
                     IEKFDKDLPHSNDTDLIELKNAISK"
     gene            complement(15341..15571)
                     /locus_tag="PC523_000617"
                     /pseudo
     CDS             complement(15341..15571)
                     /locus_tag="PC523_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(15562..15762)
                     /locus_tag="PC523_000618"
     CDS             complement(15562..15762)
                     /locus_tag="PC523_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474179.1"
                     /translation="MNDGYISYTFNESYTKDEDETAYCTKVTDTLIELFKIDMETQKY
                     NPQGNASLEGAEFTWKSYVGYN"
     gene            16380..17717
                     /locus_tag="PC523_000619"
     CDS             16380..17717
                     /locus_tag="PC523_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2474180.1"
                     /translation="MESFIMLDMFITIVSSIIELWACKKVFDYTSKVKTSLINLYFLI
                     STFIIISTFYTNVGADNRILICIFVMFVFYKLNYDVNIYKCLIVLFSYWAILVCINAL
                     SMIVIVSINNLSSMNILLNDNFYRYETIVLGNIVLIVLLYIYKFISNDLQVTKREIYM
                     CIPIVANMISFFIFYKYILKLLDISSATKLDILIMVILLTFSNIALIYSLVKILKNNK
                     KYIELTKLQEKINAENKYYSNIKKYYIKTKELNHDMKNHLICIKEMNKKNIDTSNYIN
                     NIEKKLDEYSTVFDTGNITLNIILSEKKELCIEKEIDFTININFSDCNFIKEEDTCSI
                     FANALDNAIEACEKIKNDDKKIKLQGMIVNKFFVIKITNTKSNDILLKKNTFLTTKED
                     KEFHGLGIKSIKNSLEKYGGAISINFSENLFCLNMMIPIRKSQKDSNIAYENS"
     gene            17760..18347
                     /locus_tag="PC523_000620"
     CDS             17760..18347
                     /locus_tag="PC523_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDF2474181.1"
                     /translation="MIKSCADKIAFLFVLNQVIEKDDLEICSYGLEILLSSIINTLSS
                     LMIGLILGRLIYSIVFLISFCIIRQFSGGYHANSNKNCILIFNSIFFISMVLGIILND
                     SNPIPLFLVILSSFNFLSIFIFAPVCHANNPLNETKYKKNKLMSRIISITLFLIILLG
                     CKYLSFYNYVVYTFLALLWINLLLIIQIIINKKII"
     gene            18360..18494
                     /locus_tag="PC523_000621"
     CDS             18360..18494
                     /locus_tag="PC523_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDF2474182.1"
                     /translation="MKKILYHFMKACGVLAMFVAFLSANTTSLWHVYQPKTPEKLKKF
                     "
     gene            complement(18688..20253)
                     /locus_tag="PC523_000622"
                     /pseudo
     CDS             complement(18688..20253)
                     /locus_tag="PC523_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280575.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            20701..21522
                     /locus_tag="PC523_000623"
                     /pseudo
     CDS             20701..21522
                     /locus_tag="PC523_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(21547..22167)
                     /locus_tag="PC523_000624"
     CDS             complement(21547..22167)
                     /locus_tag="PC523_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2474183.1"
                     /translation="MSDNLVNDTNSTRLGGIDRYDTNKKIVNKFYNGAKEFYIASGTD
                     LVYALVGSTIAKNDPIILVDTNSDKNILEGSSKITAISIENSSIIQQCIDVSNGIKKY
                     GWIKLGDSWHYYNKDGTEVSNYDLALKKANKVLGDKYEAYLIENTDSLPNFAQTYLDE
                     CYFFGITYKIDDSDSAILVNKKNFYAFQIDSVGRISELEKYSDPSF"
     gene            22206..22334
                     /locus_tag="PC523_000625"
                     /pseudo
     CDS             22206..22334
                     /locus_tag="PC523_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(22416..23777)
                     /gene="rlmD"
                     /locus_tag="PC523_000626"
     CDS             complement(22416..23777)
                     /gene="rlmD"
                     /locus_tag="PC523_000626"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDF2474184.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEEACKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMNEFKDMLLGIDLKSELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKRYGYEIRMVKCMDMFPNTPHCETIVKL
                     VRE"
     gene            complement(23804..24526)
                     /locus_tag="PC523_000627"
     CDS             complement(23804..24526)
                     /locus_tag="PC523_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDF2474185.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYVSATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEIKLIEER"
     gene            complement(24526..24855)
                     /locus_tag="PC523_000628"
     CDS             complement(24526..24855)
                     /locus_tag="PC523_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HDF2474186.1"
                     /translation="MPQIKIRGINEDDICKISEKMIDDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEIAWFDRGQEVQDTVARIITDSIRTNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            25185..26111
                     /gene="fba"
                     /locus_tag="PC523_000629"
     CDS             25185..26111
                     /gene="fba"
                     /locus_tag="PC523_000629"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HDF2474187.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(26726..28357)
                     /locus_tag="PC523_000630"
     CDS             complement(26726..28357)
                     /locus_tag="PC523_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2474188.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     KNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNKNNVVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLKGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNIIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(28839..29876)
                     /locus_tag="PC523_000631"
     CDS             complement(28839..29876)
                     /locus_tag="PC523_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2474189.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(29895..30680)
                     /locus_tag="PC523_000632"
     CDS             complement(29895..30680)
                     /locus_tag="PC523_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2474190.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEYDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKIGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(30713..31846)
                     /locus_tag="PC523_000633"
     CDS             complement(30713..31846)
                     /locus_tag="PC523_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDF2474191.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAAGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(31894..33021)
                     /gene="hadC"
                     /locus_tag="PC523_000634"
     CDS             complement(31894..33021)
                     /gene="hadC"
                     /locus_tag="PC523_000634"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HDF2474192.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTKLVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(33021..34247)
                     /gene="hadB"
                     /locus_tag="PC523_000635"
     CDS             complement(33021..34247)
                     /gene="hadB"
                     /locus_tag="PC523_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HDF2474193.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEMAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLIEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(34262..35062)
                     /gene="hadI"
                     /locus_tag="PC523_000636"
     CDS             complement(34262..35062)
                     /gene="hadI"
                     /locus_tag="PC523_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HDF2474194.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(35101..36300)
                     /gene="hadA"
                     /locus_tag="PC523_000637"
     CDS             complement(35101..36300)
                     /gene="hadA"
                     /locus_tag="PC523_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HDF2474195.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKGQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            36994..37992
                     /locus_tag="PC523_000638"
     CDS             36994..37992
                     /locus_tag="PC523_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HDF2474196.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENIVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLIKTEDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            38366..38893
                     /locus_tag="PC523_000639"
     CDS             38366..38893
                     /locus_tag="PC523_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HDF2474197.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASIVAGIIGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            38920..39516
                     /locus_tag="PC523_000640"
     CDS             38920..39516
                     /locus_tag="PC523_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HDF2474198.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAMFIKEKSSIKIRINTNG
                     LSDLIHNKKTAIMLKDVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPKEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(39581..41164)
                     /gene="asnB"
                     /locus_tag="PC523_000641"
     CDS             complement(39581..41164)
                     /gene="asnB"
                     /locus_tag="PC523_000641"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF2474199.1"
                     /translation="MINLEEKYSTRKDTCMIKILEYNGHIRNWKVLCEELGIDSSLGR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYKTEN
                     GKLLYSSSIRKLMEQPGFVKELNEEMLQMYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIERYWKPEFHPDYSKSIEEWTDEIHTTLQKIMPEIKSEDETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEEWFDESKLAQQTTDILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNVYHKAKCYGENLKTFYI
                     GNTNIMKEDEKRKILKKYNPDVLPIKLTKRIYEETEELDTLTNMSNIDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRMPSEYKVKGGENKIAFRKAASKVLPEEIA
                     FRNKLGFIVPIRIWMADDKYNQDIRDKFNSEIADKFFNIDEINEIFNDYISGNSNNWR
                     KIWTIYTFLVWYEIYFVKC"
     gene            complement(41731..42567)
                     /locus_tag="PC523_000642"
     CDS             complement(41731..42567)
                     /locus_tag="PC523_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2474200.1"
                     /translation="MNYIIKKVLNSSVVLVHDANDNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDISVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSVDDLLFNQMKRSNPKEMSIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
     gene            complement(42591..44057)
                     /locus_tag="PC523_000643"
     CDS             complement(42591..44057)
                     /locus_tag="PC523_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2474201.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGSKGWSVSDVAKAHFDVDV
                     KDYKSNNEITTRDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDTEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEGVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLAAQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEQDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDVTSGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRIDYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            complement(44060..45922)
                     /locus_tag="PC523_000644"
     CDS             complement(44060..45922)
                     /locus_tag="PC523_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2474202.1"
                     /translation="MDYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVDRAQFQVVIGNSVVDVYDEILKVCPLDTNDSAIKTEEKINLGTRFIE
                     FIINIFQPLVFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLIAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPSIAYNAQVFPAILCVS
                     FLSIMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAVLPFMVATGMHKALTPYAINTIGKLGYEALYMTASLAHNISESGVC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAILGVVLSSAISGIFLG
                     LFTVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNIE
                     QSSIENVVEEEPIEGKEVNILVSPVEGKVIDLSKVSDELFASKTLGEGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNSNGADYDYKVLNQSYGNVTKGSILFDVKGGI"
     gene            complement(46169..47032)
                     /locus_tag="PC523_000645"
     CDS             complement(46169..47032)
                     /locus_tag="PC523_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2474203.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDKVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFVKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKVIWEIFNNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            complement(47087..48019)
                     /locus_tag="PC523_000646"
     CDS             complement(47087..48019)
                     /locus_tag="PC523_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HDF2474204.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKYVISHDYEYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENSLDEFTTLTEATHWSGTTYGGES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEHLKTLLCVGGVHFEK
                     SFSDIIKNKEFNISIGHVLPNQWIVSGMYDDESGLEKLEKCINSIADGIDGIVFHDKL
                     KGTYKEQCRKLGEKLNIPVFKHKILKNPKDLPIW"
     gene            complement(48105..48656)
                     /locus_tag="PC523_000647"
     CDS             complement(48105..48656)
                     /locus_tag="PC523_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2474205.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDVDHFIEDDGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYVVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYV"
     gene            complement(48881..49372)
                     /locus_tag="PC523_000648"
     CDS             complement(48881..49372)
                     /locus_tag="PC523_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474206.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMNDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            complement(49400..49960)
                     /locus_tag="PC523_000649"
     CDS             complement(49400..49960)
                     /locus_tag="PC523_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2474207.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVNNWIDIEKIIKQGI"
     gene            complement(50112..51269)
                     /locus_tag="PC523_000650"
     CDS             complement(50112..51269)
                     /locus_tag="PC523_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2474208.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVLIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDNILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASRNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKSRTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            complement(51306..52247)
                     /locus_tag="PC523_000651"
     CDS             complement(51306..52247)
                     /locus_tag="PC523_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474209.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDKKKNEEISNNKNL
                     NNKQNKNIQNKESNEEGSNNIKEKNNSDSNVSVNNKSEKQEDTTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNTETNSIN"
     gene            complement(52334..53026)
                     /locus_tag="PC523_000652"
     CDS             complement(52334..53026)
                     /locus_tag="PC523_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2474210.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYENMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            complement(53135..53947)
                     /locus_tag="PC523_000653"
     CDS             complement(53135..53947)
                     /locus_tag="PC523_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDF2474211.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            complement(53998..54909)
                     /locus_tag="PC523_000654"
     CDS             complement(53998..54909)
                     /locus_tag="PC523_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2474212.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYIFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVRIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIKAGKIELNNEIFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLHQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            complement(54968..55699)
                     /locus_tag="PC523_000655"
     CDS             complement(54968..55699)
                     /locus_tag="PC523_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDF2474213.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFEKLNDVYKSLPVGNCLGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTKKNPCPFKDNNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMTLDSNIYSSGCIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEPEIRKRTIDRIKRIILV"
     gene            55788..56039
                     /locus_tag="PC523_000656"
     CDS             55788..56039
                     /locus_tag="PC523_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474214.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            56273..56692
                     /locus_tag="PC523_000657"
     CDS             56273..56692
                     /locus_tag="PC523_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474215.1"
                     /translation="MQIYFRVKAIGKRKPMLKLAPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIVNFLTNEEIESQANIGRVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSPINLKSGDIFTFIRLTLLAGRMF"
     gene            56717..61774
                     /locus_tag="PC523_000658"
     CDS             56717..61774
                     /locus_tag="PC523_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HDF2474216.1"
                     /translation="MLERTRVEKNPKIIENLKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISSYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQEVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMISKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEVYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETSFDLSNTPIDKDFNARKEQFESLKHTLENLNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNREIVLTLLRKLELTDMELDKITNLLSLKTSSIRQTVIKILLEQDDSKLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTDRKELYKKLILLIKDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDNSPSLSKLDKIISNTSLSTNEDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLNLDYKLILDILFYSISTCGYNEIEKSWF
                     SSIYSNVLDSKKQNKISTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEVYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTKEQVNREMISGYNATNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPQSFMDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACAIAMENLA
                     RSAGFEDINRMMWALETEKLNEVAYIFKPKNIDRTSLWIDVDEKGKTSIKVQKGDKAL
                     KSVPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGEIYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQVQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PIIFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKVNNVNFEGSHA
                     LIEGTLGEYSVHMGSGIVHKKGIGALNILPVHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            62089..64656
                     /locus_tag="PC523_000659"
     CDS             62089..64656
                     /locus_tag="PC523_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2474217.1"
                     /translation="MNLRFTRYIKSYKARSLAIAVSIILATSLVVGIGTLSRSAQQAD
                     VDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYTSTDVNEKLPINILYAN
                     KNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETFKVV
                     GILEDRFIEKQKGQCEIFSNLNESKLNDFTTYIEFNEGSDINTNIDNIIKNAMLDEKS
                     IGRNSMLIESTMKNGTLDNASKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLRAIG
                     STNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFVIPD
                     KIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTTVNYLISKLTLNM
                     SVTKSISAKNIFRNKKGFIIIVLSMSIGGIIVIKDNYKYSFLDVQNKSTQEQTYMNAD
                     FILSNVYLNEIGSNTSFKDIKGFSDSQIDKIKNIDGIDKVKTASILNTRIEVNKINRL
                     DYYEAINSNPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINLKEM
                     KKENLAIVYVPEISKTQKYTKTFTPGEGTPAIDIKVGDTIKVKYPKGGMDTGLYESLK
                     DNYEYLEYEFRVGAIISYTFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLVYADLN
                     ENANKKKINNLLGKIGSEVSGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFIISVL
                     NIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGLIIQF
                     KMYYTQGFVSYGLGFSIDYKIYILVVVANIIVGILATYIPSRKINKISIVEAINITE"
     gene            64668..65339
                     /locus_tag="PC523_000660"
     CDS             64668..65339
                     /locus_tag="PC523_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2474218.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVAGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIEDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKVDKIISIIDGE
                     LKLTL"
     gene            65366..65707
                     /locus_tag="PC523_000661"
     CDS             65366..65707
                     /locus_tag="PC523_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474219.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(66060..68702)
                     /gene="adhE"
                     /locus_tag="PC523_000662"
                     /gene_synonym="adhC"
     CDS             complement(66060..68702)
                     /gene="adhE"
                     /locus_tag="PC523_000662"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDF2474220.1"
                     /translation="MEKKENVVEKSSVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNAKIVGQPAHIIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTASSGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGNTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLEGLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
BASE COUNT        23398 a        11638 c         8699 g        25503 t
ORIGIN      
        1 aaaagatata caaactaata agttgtaatc tcttttaaac tctaaattaa aattatttta
       61 tacaatataa ttaaatatat tgcttataat aaaaaccata tttaaatttt agattggttg
      121 aaaattatta atattcattt ctcaactcag cttgcttctc ttcttttttc ttgttatctc
      181 ttgaatgtat tctttagtta caaggtttta cttttctcat atattataaa gaaaaatagt
      241 tttttacaaa taatccattt aatactatcc ttacttccaa cctaaatttc ctctattcca
      301 atttctttta agtattcata tacgccatta taaaaacttg gttgcattgt aatatcactt
      361 tcatcacctg tcggaacaaa tataacaaaa ccttgtcttg cccttgtaag aagaactcta
      421 tatgcatttt ttaagtatag tatattatcc tctttattga tattctgcca tttagttcca
      481 ttaaaattat agtattcaaa ttgctcattc ttaaatctta agtttccatc ccaacaaaca
      541 attgtccaat ctagctctaa gccctggata tcaaactctg tcgcagtttc ttctaaaaaa
      601 tatgaagctc gtacatcatc cttaccatta agaaaccagt ttttcgcatc aactttactt
      661 tgtacccaaa ccccatactt tcgaagtcgt ttagcgccag aacttgcagt cattccgtaa
      721 cgctgtgaac cttttgattt atctctcacc cactttttag ctaattccaa atccctagta
      781 acacatatag gataatcttt tttaaactga ttatataatt catttgcagc aattttatct
      841 acatctaaaa cggctttaac aaaggctgct actttttcac ttctaaaaga acgtaatgat
      901 acagataggt gcaaatcgtc aattattcta taattaacgc cttctgttaa ttcatcaaaa
      961 gaattacctt tagaatattc ttcatcaata attttatttg atatgtaaac atcccaatca
     1021 gaataattat ttcttagtga tgcaaaccaa cctgatattc catctgactc tcctttattt
     1081 atctcttggc ctccaccaat caaacaaaca atagtcgccc atcctatatg acgatttaaa
     1141 atactaatga gaaattcagg ctctgacata ttaaaatcta atacaccttt ttttcttttc
     1201 ataaagttag ttaaattagg ttcatcccat gcgcgttgtg cttcatcaaa aattgtgact
     1261 ctttcaatag gtggtgtatc aacagatata gcttcatctc taaaatgatg gattatctga
     1321 ataaattctt ttgcttttcg tatcgcttca gtttttcgtg ttccatttct ggttacatca
     1381 tcacgagcta atgcttcttg taaaacatca actaatggac catttccaga aaggaagact
     1441 gcatgttcat tttcatctac cttttgtcgc tcaatagcaa tattaaggcc agcaagcgtt
     1501 ttacctgctc caggaactcc tgtgataaaa catattgatt ttttattatg aatcttacta
     1561 tagtctataa ttttatttat agctttagtt gtttggttta aatttttagc acttgcatca
     1621 tttcttgaaa tatcttcaac attatggccc atatataaag cttgtgcagc ttcaataatt
     1681 gttggagttg gcatgtataa tgagtccatc caagcatctg gattaaatga acttttgtta
     1741 tatgtagtac aaacctcctc tatatacttt cctatattaa actcattact acaataagtt
     1801 tctaaaatat tttccttcat caatcttatt ctttgatttc tttgaggagc ttttgtacaa
     1861 ataagtattg gtactaaaag tttatcatga ctttccttgt gaaaacaatt aaggtcaagg
     1921 gcatagtctg taacttgttc aattgcatac ctaggataag tatcttcatc aactttaaat
     1981 tcaaggagaa aaacaatgcc tttgtaaatt aacacattgt caattctact gcctattcgt
     2041 ggtattgtat actcaaataa taagtgtcca tctttgaaag ttgagagctc tctttttaat
     2101 atttcaattt cctttttcca tgtattcttt tgcaggtcat tagcctcaaa ttgatcatta
     2161 catgttatct caccaaatat agtaaaaata tctttaccta taaattcatt tacgctactt
     2221 gaataatatg ctcttttcat acatcctcct cctttatatc taattaacct tatagtctac
     2281 atctatccta cagtctaaac tataacccct atgatatcaa tcctgtagtc taaaatctga
     2341 ctcctatact ctataaatac actgaataga tatgtttatg tatggtactt tgataataat
     2401 ttttctttgc taagtaaatt atcaaatttt caacaactat ggatatttct gaaaaatgga
     2461 ctcagaaaat tagaaattgg actcaaattt tagctcattt aagtatttat tttgaataaa
     2521 aaaatttaac tctaagttaa taaacaagca tttcaaaaaa tcaaaaagtt ttctgaaagt
     2581 gataataatt ttgcaaaaat agaaaaattc aaaaaaggct ataattttta aagtcatagc
     2641 tttaccaata aatgtttact tgtcacttaa aaaataaagt tttgtttaaa gaatacatat
     2701 aaacatttcc agactcagat ttatttaaaa tttatttact aagaagtttg atactttctc
     2761 catcaccttt tgtacaataa gttatgtgat atcttataac actcatctca agtcaataaa
     2821 ataataataa attttattat atactagcaa gacttatcaa acaaaaagaa aaaacatata
     2881 gactatttag ttaatatgtt tttccatata ttcttattat attataaaat ggatagcgca
     2941 tcatttaaat cattgattaa atcttcacta ttttcaagac ctaaacttat tctaattgtg
     3001 tatggtgaca catttaacgc tttggcttgt tctaaagtca tttcatttaa tatagctata
     3061 tttggagcaa acactaaact ttcatgacct ccccaactca ctcctatttt aaataaatct
     3121 aatttattta caaatttttt aatatcattt acatcctttg cgtctatttc aaaacttaat
     3181 aacgcagtat agttaagcaa ctgtttcttt gctagttctt tttgagtaaa atatttagat
     3241 aaaagtacat atattctcca agattataat tagtatatag tgtagtcatt aaaaagttca
     3301 ttattgtatt atcattttcc aacaaagata taatataatc aaaaaactct ggtttattat
     3361 aaaattaatt atgatgtctt cttcgttact agcttctatt tcatgaataa catgctgatt
     3421 taaaaatatt aattctcctt gttttaaatt aattttatat tcatctatag tttgtaccaa
     3481 ttttcctcta taaacataat taaattctat ataattatgt ttatgttttg gaaattgtgt
     3541 aaatcttgta tgttttctta tatgtatcaa ctcatctata ttcaataact tgttgctatc
     3601 tattataaat tgagaattat cagtataaat acttttatct attatataat ttccttctag
     3661 tatatttttt tcttcttctg ttatttccct caacttatca tgaatatatt gtctcatgag
     3721 atacctcctt taataaaatc cttgatttta tacgatattt ttttggagtc agactatatg
     3781 tctctttaaa ttttttatag aagaaggttt ggttattata tcccacttca atagaaatgt
     3841 cttctatact catatctgta ctttttaata atgtagttgc ttttttaagt ctttgttcgt
     3901 ggatgatatc cttgaatgat ttacctgttg tcttttttat taaagtactt aaataatttg
     3961 gatgaaaatg aaatttttta gcaacctccc ttaaagttgt ttgtgcataa tttccctcta
     4021 tatatttcat catttctgca atattatcta tattaatttt actatcagat gttaaagttt
     4081 tttcattttt agttttatgt atctctaaca attcataaaa tattaatata atgtaacaat
     4141 ccattatttc cttataacac attgatttat tcaaatattc cttagttagt tcagctacta
     4201 gtcgtttcaa tttaaaatta tttttagcat taaatatata ataattatca catctttttc
     4261 ctttgtgtat agctcctgct aaaaaattat atattaactc attagacgaa actctagaaa
     4321 aaaagatgca tcaaaatatt ctctcctcat tatacaatta ataatgatgt cattttcttt
     4381 tactgcattt attgaatgtt ctacattcat atccaagaga catatttcac cttcttttaa
     4441 tatggtttct tttccatcta taatttgagt acatgaacca ctataaacat aaattaattc
     4501 tataaagtta tgtttatgtt tgggtatatc tataaatcta tgatgtttgg ataccataat
     4561 gtcttcatct aaagaaagta tatttttatc tgttagtact atagttccat ttacttcttc
     4621 ccaacataaa atagtatgca atatagacat tattttttta ttatctaact tttttacatt
     4681 attagtatct atacattcta atatgtacct ttctgcttgt gttttttgtt taagtatatc
     4741 ttctagcgtt ttatctttca tttatatccc tccagaatta gctatacttt attacagttt
     4801 taaattagat taagaatatt ctttatttaa ttgtatcata ttaatttttg acagtaataa
     4861 tgttaaatta tgacatttaa aaataccttt ttttgtttta aaataaattt ataagtaaac
     4921 atttaaaatt taggaggtat ttttttatga atacagtaca taaatgtttc aagatgaagc
     4981 tttttaaagg atttgaaaat gaatacaaga aacgtcatga tgaattatgg tctgagatga
     5041 tagatatgat tcatgaatat ggtggtagta actattctat ttttctagat gaagaaacca
     5101 attatttata tggatatata gaacttaaag atgcaaaact atgggataaa agttcagaga
     5161 cagcaatctg caaaaaatgg tggaattata tgtctgatat aatggaaacc aatgaggata
     5221 atagtcctat atctatagaa cttaaatcta tgttttattt aaaataaaga caagaattat
     5281 tataaattat aaaaatgggg atgtatcaaa ataaaaactt gagttatctc cttttttata
     5341 taaaaagaga agtgattcct aaaaacctct aacctatatt ataattgttc tgtgaaaaac
     5401 taaaaataca tttcgattat agtagctaca aatgctataa acaagtggta tttctactag
     5461 acattgaaac aattattctc aaagataatt cagtaagact atttaaccta actttagagt
     5521 aattgatata tcttatgttg ctgtttatat taattaaagt atataattta tactatgatt
     5581 actattttct ttcaaactta gaatatgttc ttgctaattt ttttatttct cttgcgttgc
     5641 attctttgtg ttcccttata tatgttaaaa ctgaataata taattatgta ttatctacat
     5701 ttttatttac tttttttctt atctagcata ttcaagatat tgaataaact gttttaagca
     5761 aatttaaata gtaggtatta gataaatatt ttgtttgaca atttgaataa gtgactagta
     5821 ttcctataat tagtattata cagtattaat cataggaata tttatgttaa ggtgatttaa
     5881 ttttcaataa gtatatataa agagtactct ataaatgaat tttacaaaga aatttaatta
     5941 agtaaatcta tactaacaaa tatgttagat agattagaag ttcaggggaa tatataaaaa
     6001 cttgtgtgat tgtaataaaa gaattacact catattaaac actataaaag tagataatgt
     6061 tattaaaata actgaaaatg caataaataa aatagataca atattttata gtaattttga
     6121 aattgaagac atgaaaaagt tgaaaaatat ttagagcaaa tatttcctaa tctcaaaaat
     6181 atatattaaa taggagtgtt gaaaatgtct aaaataatag catttgtagg aagcccaaga
     6241 aaaaatggta atgtagatac aataattaat gaaataatta aaggagcaaa taaaaacaag
     6301 gttgatgtaa aaaagtatta cttaaatgat atgaatataa aaggttgtca gggatgttta
     6361 tattgtagaa aaaatcattc atgttcactc aaagatgata tgattcaagt atatgaagat
     6421 ataaaaaaag ctgagtatgt aattataggt tcaccagttt atatatgtca ggtatcagct
     6481 caaaccaaat tattattaga tagactttat ccacttacaa caatagataa aggtaaacat
     6541 atacctagat tcggaaataa aagaataatt atggtttata ctcaagcagc acctttacca
     6601 atattcttta aaagatattt tagatataca agtaagcatc tcaaaggaat gggtttaaat
     6661 cattataaaa ctataattgc tactaaagca tttgaaaaaa actcaattga aaataaccct
     6721 aaaatattaa aaaaagctta taaccttgga tttaaaatat caaaggatat taatgtataa
     6781 agtctacttt agatatttcc agactcgata tttcctgaaa gatggtgaac attataataa
     6841 ttcaccatct tttttataat tctatttgta aatattctac ttaaaaagta ttatatatat
     6901 aaaattagat gacaatatta tttgataata gagtatattt tttaaaatct ataaatattt
     6961 cattatatta ttttttataa taaacattat tctataaaag tgacgtacta ttttctcctg
     7021 aatatctgta gttgttttta catcacattc ctcaataaat ttttttaata tttcgttttt
     7081 attaaagata aaaaatgtgc atcttccttt gtatagtgat atttgatatt ttgattatca
     7141 caacttttta gaagaataca cactttattt taaatacact gctactatgt tattagaaaa
     7201 tggtgctagt ataaaagata tccaacatat actacatcac actaaaattt caacaactat
     7261 ggatacatat tcacatataa ctaattttat tcttctttca cagatttcct cattttatcg
     7321 aaacttcttg tacagacata cattccataa taaaatctac cttttaatat tatttcacga
     7381 tttcgttata gacgcgtaaa caaacaataa aaaactgtgc atctcccttg cttcattcat
     7441 actgacattt tatttataag actcttttgc gtctcctttt ggtttcatgg accaaaacac
     7501 tacattcatt acaatgacaa tcagtaatac aactgccata gccacccaaa agccgatatt
     7561 aagaccaaac cattcggtag ttccgaaaag ggtcatccaa agttctttcc agttcatcat
     7621 tgcacctcct taaagtagtt cgccgaaatg acggatatag gcttttttca aacttgttgc
     7681 caaaatcata tacagcagaa tacatggaat cagatatgca aagtatgttg caggcaatgc
     7741 aacaaatcca agcatagttc caaacacagt aaacggaata attgtcagaa ccgtaattcc
     7801 tgtcattgtt agcaaagtca ccggagctga tgcacgactc tgaaggaaag gcagctttgg
     7861 agtacgaatc atgtgaatga ccaatgtctg gctccacata gattccacga accatccagc
     7921 ttgaaacatt ccgatatact gcgcctgaag cattgccagc tctgcaccgc tgaaatgggc
     7981 aggcaaatca ttgaataaca caccattcga tacgaacaat gggcaaaata caaagtacat
     8041 aaaaatatat gttgtaaaat cgaagataga gctagttggt ccaatccaga tcataaaact
     8101 tccaacactg gaagcgtccc atttacgtgg tttggcaata aattcctcat ccacattatc
     8161 ccaaggaatt gcggtacagg ataaatcata aattaagttt agaattataa gatgaacact
     8221 catcattggt aggaagggta acagagcaga tgcagcaagc actgaaaaca tattgccgaa
     8281 gttggatgaa gcagtcattt taatgtattt aatcatgttc gcatatgtct ttcgtccttc
     8341 aatgataccc tgttccaaaa ccatcaggtc tttttccaat agaataatgt cagcagattc
     8401 ttttgccaca tccacagcgg tatctaccga aataccaata tcggcagctt tcatagcagc
     8461 agcatcatta ataccgtcac ccatgaaacc gaccgtatga ccattttcac ggagaacaga
     8521 aaccacacga actttctgat cgggggttaa cttcgcaaat acatctgtag aatctgccac
     8581 tcttgcaagc tctgcatcgc ccatttgttc caaatctgaa ccgagaagca tattgcgaac
     8641 ctctagtcct acctgcttgc agatagttcg agtcactttg tcgttgtcac ctgtcaaaat
     8701 tttggtgata acaccatggt ctttcagagc tttaatagca tctgcagtag attcttttgg
     8761 aggatcaagg aatgccagat atccaatcag taccatctca cactcatctt taacaccaaa
     8821 tgcgccgatt ggggaaggat tactcttctg tgcaatcgct agcactcgga atcccttatc
     8881 gttaagattg gccactgtct ccaaaattct gtaacgaatg tcatcagtca gaggctgcac
     8941 ttctccatta cactctgcaa acgcacaaat agagagcatt tcttccacag caccttttgt
     9001 taccatctgg gtcttaccac ctttatcttg taccactgta gtcagacgac ggcgtgtaaa
     9061 gtcaaaagga atctcatcta ctttcacata gttttcggac aggtctgtga gacgagaatc
     9121 tgtggcttcc tcttcctcag ttttatggat aattgccaaa tccatcaggt ttttatagcc
     9181 agtctggaag taactattga ggtaagcgtg acgcagtact cgcgtatcgt cctcgccatt
     9241 tacattcaga tggtattcca gcacaacctt atcctgagtc agcgtgccag tcttatcagt
     9301 acaaagaata tcaatagcac cgaagttctg aatggaattg agatttttaa cgattgtctg
     9361 cttcttgctc atggaaacag cgcctttcgc aagacaggtt gttacaatca taggcagcat
     9421 ttccggtgtc aaaccaacag caatagaaac tccgaataag aaagcctcca accaatcacc
     9481 cttggtgata ccattgacaa agaacaccag tggaaccata atgagcataa aacggatcaa
     9541 tacccaagaa acggcgttta cacccttggt aaagcttgtt tcaaccgcct ctccggcaac
     9601 tgcggacgcc atagagccaa acagagtatg atcgccaaca caaacaacaa cagctgttgc
     9661 gctaccagaa attacattac tccccataaa agcaatgctt gtgtaatcag tcacgctttc
     9721 tttctgagca cttacattgg gggttttttc gataggttca ctctcaccag taagacttgc
     9781 ttgactgaca aacaaatcct tcgcatccaa aatacgaaca tccgccggaa tcatatctcc
     9841 agcagacaga tgtacaatat ctcccaccac caaatcatcc ataggaattt cttttttttc
     9901 ttgattccta cgagtaactg tacaagtggt cgtaatcata gctaacaatt tctctgctgc
     9961 atttccactt ctggattcct gtacaaaacg aagtgtaccg gaaatgaata ccattgttaa
    10021 aatgattaca acagtcaggt aatcaaagtc ctctggcaca ctgccaaaca gggaaaaata
    10081 cggaaaaatc atatccgtaa ttgtggatac tacagccaga caaaataaaa tggcggtaaa
    10141 tggattgata aatgcaccag ctagccgttt tgctagagat ttcttttttt catgagtgac
    10201 tttattggta ccatatttag cacgacttac gggtattgca tctacttcca atccacgagg
    10261 tgtggtatga aattttttta aaacttcttt aataggattg gttgccgcaa attgaatgcg
    10321 gcggttctgt tcgtcacgga tgactgtttt ttctacagtc tgacgaacag ccatacgatt
    10381 ttcttttttg ttcattgttc ttacctcctt gaattttcat aggagcaagg aatattatat
    10441 gagtgacacg gaatctccaa attctcatct aatattctct gcatttgtgg actgacatca
    10501 gagtccatgt ctttcacctc acaatcttaa aaaatatcta tgtgaaaccg caatgcggat
    10561 tattggttac gatagcggac acttttttcg atataccaga catcaaagct ggcaacaatc
    10621 aaccatgcga ttattgaaaa tataaaccag tggtttgaaa atcctggcaa aactcctttc
    10681 attaagaata taattccatt taatgcaatt attcctccaa cattacgaga aagcgggaca
    10741 atattaattt tatctttctc ttccttcggc atatttttcc acagggaaag atgacgatat
    10801 ccttttccca cggctaacca aaagcctgca aaagtaaata aaattccaaa aaagatacat
    10861 gtaaaatcca tcgcttgttc ctcctttcaa tgcttaaacc tgtttcttag tttcgtcagc
    10921 ttcataagtt taatgaggag cacaatcata gtaacagcaa gaataaacca gatcataaca
    10981 ctcacctgat accatatctt aaagattgcc attctttgtt cagataaatt ccaacccaaa
    11041 tagctacata gtattgtaaa tacagggtag aaataaaaaa gaattgcctg tttcaaatct
    11101 ttgatttctc tcctcactca caacacctcc ctttaactac taacaatata taaaaaatcc
    11161 aagtagaatc atatggctaa agtcttgcga ttttcttgcg atttggtaag aaaatagacg
    11221 gactgaaacc gaaaatggtt ttagtccgtc tttgtcatat ctgatcatta aatttatatc
    11281 caatacccca gatagtcaaa atgtatgtgg gagaatctgg ggcaggttca attttttttc
    11341 gcaacttccg gataaaagcc ataatgttgc tgttatccag aagataatct tcctcccata
    11401 cggcacgata aatctgttcc tttgtaaaaa cttcaccttt gttctggaca agaaagtaca
    11461 aaatatcaaa ttcttttgga gtcaagttga cttcagaacc ttttatcagt acttttctgc
    11521 ctcttggata aatttcaaga tcccctgcta ggattctgtc tgacagagta cccactggac
    11581 aatcgctttc ttctgatgac cctaacaata acgaagatac ctctccgcga atcatctcct
    11641 gaatgccaga aagtaaatcc ttcgcataat cactgtctgg tggatctctt aatagttttt
    11701 ctaatagcca gatttcaaga gccgaatcca tcggaatata actaatattc tttttcatca
    11761 tgaatgcctc ctcttaaatc aactcttgat attttttctt ataaaaatat ttagctactg
    11821 aattcatcag catatagaga aataccacca gcagtaaaaa tacgaaatac caaaggggca
    11881 atttcgtcag tccaaacagc gatgcaccac cagcaaaagt aagataagta aagccaataa
    11941 tccctactag agttatagaa atgactggtg cagatgccct gctctggaaa aaaggaatct
    12001 tggaagttcg cagaaaatgt aaaatcaaaa cctgtgtcca catagattcc aaaaaccatc
    12061 cagtctgaaa cagagctaca tactgaagtt tcagagaagg atctgtaagc tgcagatagg
    12121 ttgcccctcc acatagcatc ggacatagga aataatacaa aaacaggaag gtaacaatat
    12181 caaacagaga actgattggt ccgaaagata gcataaactg tcttagtgtt ttgccagacc
    12241 agtctcttgg agatagcatt tcttcctcat ctacattgtc ccagggaagc acaatacaca
    12301 acgtatcata cagcaaattc aaaagcagaa tctgaattga agtcataggt aggaacggta
    12361 aaaaggcact ggcacaaatg atggaaaaaa tatttccaaa gttagagcta gcagtgattt
    12421 taatatattt cagcatattg gtaaaagttt ttcgcccctc cattacacct tgctctaaaa
    12481 cattcaagtc cttttgcaac aaaaccacat cagcagcatc ttttgctgca tccactgctg
    12541 tatccacaga gattcccaca tttgcttcat ttagtgctgg aatgtcgttg actccatctc
    12601 caagaaatcc cacggaatga ccattatcct gcatagcaga gaccagacgc accttatgtc
    12661 ccggagtaag ctctgcaaaa acatgagtat gttccacagc ctttttaagt tcacagtctg
    12721 tcatttcgtc cagttgtgta ctagttaaga tacgctctgt agaaataccc acccgacggc
    12781 aaacagatac agcaatcgct gcttggtccc ctgtcagaat cttaggaatc acttttaatc
    12841 ttttgagtgc agttacagac tcctttgctg tctgctttgg tgcatcaaag aaggatagat
    12901 acccaactag agtcatgccc cattcatcat caggagtaat tttcttttgt gctcctacat
    12961 tttttcgtgc caccgcaata accttcattc catcctgtaa catttcacct actacagaag
    13021 atacactctg tctcgcatcc tgccccatgg ggagaagttt tccatgatat tccacataat
    13081 tgcagcgtga aaagatatgt tcgatatccc ctttcataat aagctgacta ttcccattgc
    13141 tatcctgtac aagtgtgctg acaaacttac gagcatagtc aaatggaatt tcatcagctt
    13201 tttgaaattt tgtcagaaga tttgcataat gggcttcccg ccctggcatg gtattgcagg
    13261 caagaatggc attatcaatg gtattgcaga caccagaatg ataggagcta ttcagataag
    13321 caagatcaag aacctctgca ttttcatttc ccaagatatc catataatat tccagcagga
    13381 tactttcatt ggtaagagtc cccgttttat ccatacaaag cacatccatg ctgccaaagc
    13441 tctgcatagc attaatattt ttgataatag tctgcttttt gctcatagca agacttcctt
    13501 tggcgagaca ggctgtaata accataggaa gcatttctgg caccagacct acagcaactg
    13561 acaaggcaaa agcaaaggat tccatccatt ttccaccagt gattcccagt aatataaata
    13621 caatgggaac taatatcgcc atgaaacgaa tcatcaccca ggcgatagaa tcagctcctt
    13681 ttttaaatga atctttatca tcagaatccg gtttggtaaa tcctccatac agggtatctt
    13741 tacctactgc caacacaatt ccttcccctt ttccactgat gactgtggtt gccataaaag
    13801 cgaggttttc aagctgagtc agcgtctcct gttctccgta actgagtttg cgacagctct
    13861 tttcaaggat agcactctca ccagtgattg cagcctgaga gataaagaga tctgtaatct
    13921 ttgtcaatct gacatctgcc ggaacacaat cacctgcgga gaatagtaca atatccccta
    13981 ccactaattc ttcgccagga atctctatta actttccacc gcgcctcact gtgacgcttt
    14041 catgaactag gcgatcaagt tgagcagcag cgctctttgc tcgtagctcc tgaatcagac
    14101 gaattacgcc actaattagt atcatggaaa agataataat ggcagtagta gcatttctcg
    14161 caaaattgga tgctaagaaa acatctgtaa caagagaaac aatgcccaga acaaaaagaa
    14221 tcacatgaaa tgggttaata aatgctcgtc tcaatcgttg catcgtcgta tcattttttc
    14281 gcccattgaa gctgttcgct ccatatttct cccgcattga accaatttgc tcaatagata
    14341 gaccatctgg agatgagcca atgtctcgat agatttctga tatttcacag taagcgtatt
    14401 ttttaatacg actatcaaat agtgttgctt ttgacacgaa acatcccctc ctttataaat
    14461 ataaataaag atatccacat tataacacag tgttctaaaa atgctatatt tatattcttg
    14521 cttttttctt gcgatttact tcgatatggc atttttcagc tcgatcaaat ccgtatcgtt
    14581 agagtgaggt aaatccttat caaatttttc aatcattcct tctaaatttg gagcacggat
    14641 ggatgaagca agcactttct catagagctt tcgcacagac ataggtattt ccactttaca
    14701 catatgagtg cagacaaacg tcttcttggt tttcaccttg accatgtatc aaatttaaat
    14761 taactactta gagaagtttc tcatagtgat atatgtatta tgagtgatag aatggcaaaa
    14821 gtggcagtat agaaaacgag tgtaaaagca atacaattag tttgtatgga atatctgaac
    14881 tacataatta accactctgt agtttataaa acaatacaat gcgtaaattg gaacggaaca
    14941 gaaaaaacca atagtatttt taataattcc acttcactat tgattactct tatctattat
    15001 tgctatttac aacaaaataa aacaacagaa attttaaata tgttgactgg cataattcat
    15061 atgtatagaa agtctacagt taagatgcga tttttctaat ccaaatatat taagaaagga
    15121 attttacgaa gccattagta ctttgtttaa gatgtctttc ataaatataa agaataatgt
    15181 tcttaacaaa ggcgtgtaaa tttatataga aatgcatact ttaaataatt atcccacaat
    15241 atttcagtta ctgtctttat gtcccacaag ttcaaactac gacacctaca ttgcctttgt
    15301 ttgattgtca atgaatttca cagcacctta actttcacat tgtacccaac ttctcgatac
    15361 tccatacaat ctcatctgta actgtgtagc tggtcggtat atccgttcta atggtggtat
    15421 ctacattcaa gtcctgcaat tcctttgtca gataaccgct gattgtctac atccttactt
    15481 cgatagcttc tttgctacta ttccaagtgt agtagaactt tacaaagttt ttgacgaagt
    15541 ttttaatccc gttggtatca ttcagttgta gcctacataa gatttccatg taaactctgc
    15601 tccctctaaa gaagcgtttc cttgtgggtt gtatttctgt gtttccatgt caatcttgaa
    15661 aagttcaatc aaagtatctg ttacttttgt acaatacgct gtttcgtcct catctttcgt
    15721 gtagctctca ttgaatgtat aactgatata accgtcattc attacttttt caacaatccc
    15781 gatacgccct gtcgattctg tccaatacta tttatcagac gcatgaacca ctggtaatga
    15841 cagtagcaaa ggcaagaacg cccatacaca atcgctttaa tatttttttc ataattctaa
    15901 atactcattt cattttgtaa ttgggtaaaa aaatagacac ttattttgaa gtgaacatct
    15961 tgctttattt tagtactttt ctcttaaaac gcaatattct tcattatcta ttattatttt
    16021 atagcgagat tgatttaata atcattgatg aagtagataa tattgtttgg agaaatgaaa
    16081 gaagcactcc tatagaaaga gtacttttag tgtatattca agaatttctc taacatgatt
    16141 ataacatgtc ttatgtttta gtatgataat tttcgttcgt ttcgttcgtt ttattattac
    16201 aatgcatatt atagttttac tcatttatat taataattac atatgtagat aaaattattt
    16261 atatatatat aatatttagc aacttcatcc aagtataatc tattttatga tataattatt
    16321 ataaaagttc aaatcatttt cgtgttatta aaggaaattt atgcaaaatt gtttaagtta
    16381 tggagagttt tattatgtta gatatgttta taacaatcgt ttcttctatt atagaactat
    16441 gggcatgcaa gaaagtgttt gattacacaa gtaaagtaaa aactagttta ataaatttat
    16501 attttttaat ttccactttt attattattt ctactttcta tactaatgtt ggtgctgata
    16561 atagaatatt aatatgcatt tttgtaatgt ttgtttttta taaattaaat tatgatgtta
    16621 atatatacaa atgtttgata gttttgtttt cttattgggc aatactggtg tgcataaatg
    16681 cattaagtat gatagtaata gtttcgataa ataacttgag ttctatgaat attttattaa
    16741 atgataattt ttatagatac gaaactatag ttttaggaaa tatagttcta attgttttgt
    16801 tatacattta taaattcata tctaatgatt tgcaagtgac taaaagagaa atttatatgt
    16861 gtataccaat agtagcaaat atgatatcat tttttatatt ttataaatat atcttaaaac
    16921 ttctagatat ttcttccgct actaaattag atatcttaat tatggtgata ttattaacat
    16981 tttctaacat agcattgatt tatagcctag ttaagatatt aaaaaataat aaaaaatata
    17041 ttgaattaac taaactacag gaaaaaataa atgctgaaaa taaatattat agtaatataa
    17101 aaaaatatta tattaagaca aaagaattaa accatgatat gaaaaatcat cttatttgca
    17161 ttaaggaaat gaataaaaag aatattgata ccagtaatta cataaataac atagaaaaaa
    17221 agcttgatga atatagtact gtatttgata caggaaatat tactttaaac attatattat
    17281 cagaaaaaaa agaactttgt atagaaaaag aaattgattt tacaattaat ataaacttta
    17341 gtgattgtaa ttttattaag gaagaagata catgttctat atttgcaaat gcattagata
    17401 atgcaatcga agcttgcgaa aaaatcaaaa atgatgataa aaaaatcaaa ttgcagggaa
    17461 tgatagtaaa taaatttttt gtaataaaaa ttactaatac aaaatcaaat gatattttat
    17521 taaaaaagaa tactttttta actacaaaag aagataaaga atttcatgga ttaggaataa
    17581 aaagtataaa aaattcttta gaaaaatatg gtggtgcaat ttctataaat ttctctgaaa
    17641 atttattttg tttaaatatg atgataccaa ttagaaaatc acaaaaagat tcaaatattg
    17701 catatgagaa tagttaatat ccataattta ccaataaaat tatttaagga gaaaactaaa
    17761 tgataaaatc ttgtgctgat aaaatagctt ttttgtttgt tttaaatcaa gtaatagaaa
    17821 aagatgattt agaaatctgt tcatatgggt tagaaatttt actatcttcc ataataaaca
    17881 ctctatcttc attaatgata ggtttaatat taggtagatt gatttattct atagtgttct
    17941 taatatcttt ttgtataata agacaatttt caggtggata tcatgcaaat agcaataaaa
    18001 actgtattct aattttcaat agtatttttt ttatatctat ggttttggga ataattttaa
    18061 atgattctaa tcctattccg ttatttttag tgatattgtc tagttttaac tttctaagca
    18121 tttttatttt tgctccagta tgtcatgcta acaatccact aaatgaaaca aaatataaaa
    18181 aaaataagtt aatgtcaaga attatatcta taactttatt cttgattatt cttctaggat
    18241 gcaaatactt atctttttac aattatgttg tatacacttt tttagcttta ctttggataa
    18301 atttattact aattattcaa atcattataa ataaaaaaat aatataagga gatattaaca
    18361 tgaaaaaaat tctatatcat tttatgaaag catgtggtgt attagctatg tttgtagcat
    18421 ttttatctgc aaacacaact tctctgtggc atgtatatca accaaaaaca ccagaaaaac
    18481 taaaaaaatt ctaaaaatca ccaaaaagaa ggggctgtcg cataagcaaa ttaaatttgt
    18541 tctgtgacag ctcttttttt acttatttta ataggtatat ttttaattgt tttatattaa
    18601 gtttatattc taagtaaaat tatgcataac aaataaaggt ataaaaatac cattttttta
    18661 gataatattt aattataaac aataaattta gaatattttt tttaaatgat gaagatatat
    18721 tccatgttta ttttttacca ttttattgtg tagtctagaa atattatgag ctatcgctac
    18781 aataatacat tctgatagaa cattttcttt acctcagctt aaaaatcttc taaatttcat
    18841 atttttttta cttgagcgaa agctccttct gcttatatac ttctattcat tctaggttca
    18901 aacccttccg ggcttataat tctctttaag ttttcttttc ttttttcata aaataactta
    18961 gatacttgta agtttttagt cctattttca agaggaatct tactattact tctcttaata
    19021 caactgcttt tatattgaca ataacagcat tcttcacaag tataacaatt tatttcactt
    19081 ttatatcctg tttttgattt tctattgatt attttattag caaaagtttt tgccattttt
    19141 acaaatataa taatcatttt tttcatcata atccatatta tcaatacgcc caatctcatt
    19201 tttatatttt ctagttttac taatttcata atttgcaggc tttatgaaag acaattgatt
    19261 attacgctct aaaaatgtat agttttcctc accttcatag ccagaatcag ctactatttt
    19321 aggatattta aactttatat ggtcttcaat agattttaaa aatgatatta atgttgtagt
    19381 atcagtaggt tgatgccctg aacttaccaa aactatgtat tctgaatcta caccaaattg
    19441 tatgttatat gctgatttca attggccatt tttcatatgg tcttctttca ttctcataaa
    19501 agttgcgtca ttatctgttt tagaataact atttcgttca cccattatat gaaggtcttt
    19561 agtatatttt ttcaatctat caatataatt atttaattgc tctatagatt tttgaatgat
    19621 agattttttc tttccttttt catgaacaaa tactatattt aattcttttg atatttttct
    19681 tagtttcttt aaaagttttt ttagatgata gattttgatt ttatctttat agattatttt
    19741 aataccaaaa tcaatctcac atttcaatat gaagtcagat ctattagcag attactctat
    19801 ttttgtacca tcaataagca gagtatcctt tgaaatctcc ttattatatc caataaagtt
    19861 tgtaaactgt gcaatcaaat ttttgcttac ttgtgaaaaa taaatactac gaaatcttgc
    19921 aattgttgaa tggtctgggg cggaggctcc gcctagaaga tacataaagt taatatctct
    19981 tttgcatgct ttttctattt ctcttgaaga ataaatgcag ttcatgtttg catatattat
    20041 aattttcaaa agttgtctag gggttacttt atttcctcta attcgagaat aagtcttata
    20101 taatttttct aaattcatct cctcaataat ttgacttaat aaccttacag aatcatcttt
    20161 tggaatcatg taatcaatat ttagtggtaa tttaaattga taattatcac cataagtagt
    20221 ataatcaatt tgtatagtat tttgtttttg catacaatta ttatacaaga atcccaagct
    20281 cttttgaact tgggattttt ttatacaaaa aaatggcgta gcactatttt ctagtgcgac
    20341 agccccaact ctatattatt tagaaattat tactgtaatt ttactatttg aacatgtaca
    20401 ttcaaaatac aagtctagaa attagcttta tttgtaagat aaataaaatc agtcccatta
    20461 tcttctgtat gcaaactaat taagttattc ttttttaaat atttaattgc atatttttcc
    20521 tatatacata gcatttataa agaaagtttt atatacttaa actattatct tatttaaaga
    20581 tattaacaat aaaaacctaa aacaagaatt gtaatacaag ataaaatatt tgtctaaata
    20641 atattgctta tcactaagac aaaaactaaa ttaatataaa aatagaggtg attagatata
    20701 atgaataatt ataattctat aaataactta caaattggaa aaacatattc taatgacgaa
    20761 ataaaagaaa tatttaaatg ttctactcaa ggtggtatga taaaatcaaa tactacaaag
    20821 actctagtta tattctctga tcatacaaaa agtatatatg atgatatttg gtatgataat
    20881 acgttaatat acactggtat gagaaagaag ggtgatcagg tattaaaagg taatcaaaat
    20941 ataacacttt atgagagcga aactaatgga ataaaagttc atttatttga gacttttgtc
    21001 tcaactaaac atatttataa aggatttgtt aaactgtcag gtacaccttt tataggtgag
    21061 caagaagatg ttgatggaat aaatagaaaa gtatggttat ttcctttgaa attaataaat
    21121 aatataccta tagaaaaaaa tctattagag tataataaaa atcttaaatc taaaaaagct
    21181 agaaaaatga ctcaattaga actaaaagat aatataaaaa ataaaaccat ctacaagaac
    21241 tgttatatca aaaccatatg taagagatga gctagtttct caatatgcaa aaaatagagc
    21301 aaatgagatt tgtgatctat gtggttctaa tgctcccttt aatgctctca tggtaaccca
    21361 tacttagaat atcaccatat taagtggctt tctgatggtg acgaagatag tttatataat
    21421 actgttgcac tttgtcctaa ttgtcataaa aaaatgcata tatgtaattt aaatgaagat
    21481 atacataagt tattagacaa gataaaaaat tacgaaaagt aaattagtta aacaaatata
    21541 ataaatttaa aaactaggat ctgaatattt ttctaattca gatatcctac ctactgaatc
    21601 tatttgaaaa gcatagaaat ttttcttatt tacaagaatt gctgaatctg aatcatctat
    21661 tttataagta atcccaaaga aataacattc gtccaaatat gtttgtgcaa aattaggtaa
    21721 agaatctgta ttttctatta aatatgcttc atatttatcg cctagaactt tattagcttt
    21781 ctttaaagct aaatcatagt tactaacttc tgttccatct ttattgtaat aatgccaact
    21841 atctcctaat ttaatccacc catatttctt tattccattt gatacatcta tacattgttg
    21901 aattatacta ctattttcta tactaatggc agttattttg gaactaccct ctaatatgtt
    21961 cttatcacta tttgtatcta ccaaaataat aggatcattt ttagctattg tagaaccaac
    22021 aagtgcatac actaaatctg ttccacttgc tatataaaat tcttttgctc cattatagaa
    22081 cttatttact attttcttat tagtatcgta tctatctata ccacctaatc tagttgaatt
    22141 agtatcattt actaagttat cactcattga tgaagtacca cctattgcat aactttctat
    22201 tccaggctaa tattaaagat atacaacata gattagggca tgctaaaata tcaacaacta
    22261 tggatacata ttcacatgta actaacaaca tgagaaacca gacagtaaat atttttgaaa
    22321 gcattgtaga gtaaatttgc caccgaaaaa taattacggt ggcaaaccgg tggcaaaccc
    22381 acctttatag catcatatag atagctgaaa gcctcctatt ctctaacaag tttaactata
    22441 gtctcgcaat gaggcgtatt cggaaacata tccatacact taaccatcct aatctcataa
    22501 ccatatctct taaaatcaac caaatccaca accaaagact taggattgca agaaatataa
    22561 acaatctcct ttgcaccaaa tccacaaata tccctaatag catctttatg aatacctggt
    22621 ctaggagggt caacaataat caaatcaggc ttgtccttta aatccttaac aaccttagcc
    22681 acatccccag caataaactc acaattagta agcccattca acttagcatt ctcattagca
    22741 gcaacaacag cctcctcaac tatctcaata ccatacacct tcttagcagc accagccata
    22801 acctgtccaa tagacccagt tccactatac aagtcaaata caaccttatc attgtaatct
    22861 ccaataaaat ccctagcaat actataaaga acttctgctc ctttagtatt agtctggaaa
    22921 aatgaaaatg gagaaatctt aaacttaaga cctaaaattt cttcttgtat ataatctctt
    22981 ccatataaaa ctcttaactc atcacattgg accgcatctg ctaaaccatc attaatagta
    23041 tgaagtaccc ccaccaactc agattttaaa tcaatcccta aaagcatatc tttaaactca
    23101 ttcatatcaa aatcttcttg agaagaagtt actatattaa ccataatctc attagtatga
    23161 atcccttttc taactacaag atgtctcaaa tatcctttat gattcattgt tctatagtaa
    23221 ggaagctttt tttcattaaa aaactctaca ctagaaacta atatctttga gaagtcttca
    23281 tcaaccaaca tacactcctc aacagtctgt atatctatat gttttccttt tttatgaagc
    23341 cctaatgtca aaggtccatt ttttacttca tctccaaaag tgtattccat cttatttcta
    23401 taatatttgt tttctggact tccttctatc cctaaaaatt gaaatccaaa taaatcttgc
    23461 tttaaaaata aatccataac ttgtttttct tttatttcca attgtttttc atatggcaca
    23521 gacagtaatg tgcatcctcc acactctcta aagtgcttgc aagcttcttc agtttcaagt
    23581 ggtgagtttt ctaatagttc tatcatctta acttcagctt tcttacttct aactttttta
    23641 actccagctc tcaccttttg accacttatt ccacccttca tatggataac tctatctcca
    23701 acttggctta aagatgtacc accaaattcc atcttatcta cttcaaattc tataacatct
    23761 tttttcttca cagacattcc tccagtttac tatagtatct ttattatctt tcttcaatca
    23821 attttatttc ttcatctgtt aactctctat attcacctaa ctctaaactt tcatccaatt
    23881 ttaaattacc catagacaat ctttttaagt atactacttt tttgcctaca ctttcaaaca
    23941 ttctttttac ttggtgaaac ttaccttcat gtattgtaag ctctatctct gattcatcat
    24001 cactctttaa gatatttagc tgagatggca tagttttata tccatcatca agtactacac
    24061 cttctaaaaa agcttctaca tcttcttcag taactactcc atcaattttt gcataataag
    24121 tcttaggcac atgcttctta ggagataaaa ctctatgaga aagctttcca tcattagtta
    24181 aaactaataa tccctctgtg tctttatcta atcttccaac aggaaatggt tcaaaagcta
    24241 agtatgataa atcaattaaa tctaagaccg ttgggtcata tttatctgta gttgccgata
    24301 catatccatc tggtttattc atcattaaat atatatactc tctatatata acttcttctc
    24361 cattaaacaa tatctcatca ttttcagtgt ctacttgagt tcctggattt gaaacttcac
    24421 ttccatttac tactacagac ccttgcttac aatacttctt tatttcactt ctacttccat
    24481 agcctaaatt tgaaagaatt ttatctatac gttgcttttt agccattaaa aatgctctcc
    24541 attctcataa tatttttctt tttcaaatac tgtaaatgct aaatccatac tttctacatc
    24601 taaattagtt cttatactat cagttattat tcttgctact gtatcttgta cttcttgtcc
    24661 cctgtcaaac caagctatct caacaaaagg atatacatct gctattttac catctcttat
    24721 tgcaactgac tttatacatt ctatttcaaa gtagtctctt ggacatttta cagcttcaac
    24781 taaatcatct atcatttttt cacttatttt acatatatca tcctcattaa ttcctcttat
    24841 ttttatctgt ggcatagttt acctccttta ttttaaattt ttagtgaatt atcatccact
    24901 taattacctt cgataacgtc tgaagtggta gcttcttggt caagataact attgttatca
    24961 gtatctccaa actaataagg tagttcctac ccttatagtt agcattatat agctaagttt
    25021 cctgaattgt ttatattctt tattatttta tttaatccta ttgtcttcta tttaacatta
    25081 tacttgtaat ttatagttat tacaatttac tatatacttt ttttgattta acgttataat
    25141 ataagtgatg ggtaaatttt aaaagttaat aggaggagtt taaaatggca ttaattacta
    25201 caaaagagat gtttaaaaaa gcttacgaag gtggatttgc tataggtgca tttaacataa
    25261 gtgacttaga acaattacaa ggtgttttaa aagctgctaa agctaaaaat tcttatgtta
    25321 tgatacaagc ttctatgtca gctgttaaat atgctggacc tcatacatta gttgaaatgg
    25381 ttaaggctgc ttctgatgaa ataggtgtag acgtagctct tcacttagac catggtccaa
    25441 atatggatgc tattaaaact tgtattgatg ctggtttctc ttcagttatg atagatggtt
    25501 ctcattttga ctttgaagaa aatgttagaa taactaaaga agctgttgaa tatgcacact
    25561 ctaaaggtgt tgttgttgaa gcagaacttg gtgttctagc tggaacagaa gatgatgtta
    25621 cttctgatgt tcacaagtat actcaaccag ctgaggcagt tgagttcgtt gaaagaacag
    25681 gtgttgattc attagcaata gctattggaa cttctcatgg tgctttcaaa ttcaaaggag
    25741 aagctaaatt aagattcgat atactagaag aaatccaaag caaattacca ggtttcccaa
    25801 tagttcttca tggtgcatct gctgttgacc aaaacgctgt tgctacatgt aatgaatttg
    25861 gtggaaatat agctggtgct aaaggtgtac ctgtagatat gttaagaaaa gcatcttcta
    25921 tggctgtttg taaaataaac atggatactg acttaagact agctatgact gctgctatca
    25981 gaaaattctt agctgaaaat ccaaaagaat ttgacccaag aaaatacatt ggtgctggta
    26041 gagatgctat acaagctgta gttgaaagca agatagatga tgttttaggt tctgctaact
    26101 ctataaacta atataacttt taaatattta taaaagaaaa gagatgactt aaagtaatta
    26161 aatttatttt taagccatct cttttaagtt gtatatatct ttttatataa acaaatatat
    26221 gttatttttt tactgtattt ttaactatgg taccatctct aaacccttta gctatttctt
    26281 ccacattcat ttttacatta ttagaagtat ctgcaaatga tttatttgta agcaaacaaa
    26341 atccacctaa accaatcagt accgttaatc ataaagccat aaactatttt tttatgatta
    26401 ttatatgctt ttttgttatt ttctaaataa taccataaaa atgtttatgt gctaatacaa
    26461 aaacactttt agcgataata ctccttactg tgattggtaa gttataaatg tgttattcaa
    26521 tctagtagat tcttactttt ttagaagact ggaacaaatc atatgagtac acatctatag
    26581 tgtttctacc aaaaaacaaa aaaatatcac tggaatcaaa acctccagca atatttaata
    26641 ataaaaaata tatttacata aatctatgta cattttccta ataaagtata tttcatcaat
    26701 tctaattact ttaccaaact aacaactata catgaatatg tccactcttt atctttctat
    26761 ggatggtaga aggatatata cctataactt cagcagcctt tgttattgaa ccatacttat
    26821 ttatagcctt acttatcatt atttggtcca ctattttata tgcatcatta agattcataa
    26881 ttccatttac cactattggt gataaatcat ctgtttcatt atcaatcata tctaaattta
    26941 taagcatatt aaattcatct tctccaatta tatttttagc tgataatact acgaatcttt
    27001 caataatgtt ttttaactct cttatgtttc ctggccaaga atattcttct agtaactcaa
    27061 taaccttagg tgaaacttct acttctctat tatacttttc attgtaaagt tttaagaaat
    27121 aatttactag tggtactata tcttcttttc tttctctaag tggtggtatt tttactggaa
    27181 taacactcaa tctatagtac aaatcttgtc taaatttaag attatttcta aggtcttcat
    27241 gagaaatatt agtagcactt atatatctaa catctatctt tataggctta ctaccaccta
    27301 ttctagtaat agtattttct tgtataactc taagtaattt tttttgcatc tgcattggta
    27361 attcacctat ctcatccaag aaaatagttc ctccatttgc ttcttcaaaa agtccctttt
    27421 tgcctttttt cttagcacca gtaaaagaac cttcttcata tccaaaaaac tctgactcta
    27481 ataattcatt tggtatagct ccacagttta tagctataaa aggtttgtct cttcttgaac
    27541 tatacttata tattgttctt gctattattt cttttcctac acctgattct ccatttataa
    27601 atatagaaga atctgatgct gcagctctct taacaacttt ttttatattg tccataatct
    27661 tactttgact tatatattct gtgtcttctg atatatcact atattttatc tgagattcaa
    27721 gcttgataac ccttgaaata tctcttcctg ttagtactcc tcccttaagc tttcctttag
    27781 aatcccatct tggaactgct gtgtatgcaa taagcttccc atctgggtaa acgatatttc
    27841 tataaatctt tttcttcact ttaagcatgt ttaatattat tggttcttca actaatcctt
    27901 gtcttacaag gtctgttata tttctaccaa gtacctcagt tcttttgaag ggtaatatct
    27961 cgtcactgac agtattcatc ttttcaatta ccccatcttt gttaaacaca acaacaactt
    28021 catctatatg ctctattata tcttcaatta ctttatcaga tattagagac ttctcaaata
    28081 aataaactac attattttta ttattattat ctttcatatt gtatatatgt gcagtcatcc
    28141 ttaactctct gaaagctatt ttctttttac tatctgtcag tatttctgtt ttgtcaaaat
    28201 ttgaatctaa ttcatttata tttttaggct tttttatatc tagaaaacta aaaatactat
    28261 ctattagatg attacagtat attatttcgc ctgattcttt acataaaatg gctggatttt
    28321 ctacatgttc tataagccct attaaattat tatccattaa tattcgcacc tttcattaag
    28381 taatttattg aactacatac aaaatttatt gtttgcttac aataaatacc atcagcacat
    28441 ataatcaact ccttataata tttgtctttt caagtagttt ctttcattaa aacgtaacca
    28501 agatataatt ttaatgatta tagtttgtta aaaatttcat tttgctttta attgtatttt
    28561 atcacatatt tagtaattat ttgtctattt aatacaattt aattaaattt aagatttatt
    28621 gaaaaaacaa agataatcct tacagaatat gactcacaat ttgtaagcaa tatacatata
    28681 aggattatct agattatctt gtattataaa attttattca aacagacttt ttaataactg
    28741 acaaatacgc actagcaata acttatcaaa gggttaataa ttatgttact aactaataag
    28801 acttattcaa tcagtaatat ttatatagtt tgtatctatt aagcttttat agcttctaat
    28861 aaatcatcaa cgctatctaa tttttctata agcattggaa ctatttcatg taagtctcca
    28921 actactccat agtcagcgat ttctaaaatt ggagctgctg ggtttttgtt tatagcaatt
    28981 atgaaatctg atgattgcat acctgctaaa tgttgtattg ctcctgatat accacaagct
    29041 atatataggt ttggtttaac agtagttcct gtttgaccta cttggtgaga atggtctatc
    29101 catccagcat caacagcagc acgagaagaa ccaactactc cacctaattt gtcagctaat
    29161 ttcttaagca tttcaaatcc ttctggtcca cctaatccta gaccacctga tactataaca
    29221 tttgcatctg taagagatac taaatctttt ttagttttaa ctgtttctaa aacagtagtt
    29281 cttatatcat cttgagttat tttgtaatct aatgctataa cttcaccagt tcttgtttcg
    29341 tctttttcag ctttatccat aacaccaggt ctaactgtag acatttgagg tctatggttt
    29401 ggacaaatga ttgtagccat tatgtttcca ccaaatgctg gacgagtttg ttttatcttc
    29461 ttatcttctg ggtctatttc taatttagta cagtcagctg ttaatccagt tccaactctt
    29521 gaagctattc taggtgctaa gtctctacct atatgagttg ctccgaaaag cattatttct
    29581 ggttttattt catctattgc atcttttatt acttttgtat atgcatcagt tgtatatttt
    29641 tctaaaagag cgtcatctgc aacataaact ttgtcagctc caaaagtgat taattcttta
    29701 gctaatccat caacatcttt tcctaataat attgcacaaa gctctgcatc tacttcttta
    29761 gctaattttc ttccttctcc taataattcg ataactactg gagctatttt tccttctctt
    29821 tgttctgcaa atatccatac gtttttataa gaacttaaat cttttatatc attcatagtt
    29881 tgtactcctc caacttatat aatgtgcttt tgttttagac cgattattat tttactagct
    29941 atttcatcag ctttaccttc taagatttct ccttttcctc ttggttcagg agtaaatgat
    30001 ctaaatactt tagttggtga tgcttttaaa ccaacttctt ctttatttac atctaaatca
    30061 tcaattgtcc aaacttttac atcatgttta tatgctttta ctattttatc aacagacatg
    30121 tatctaggtg tatttaactc ttttactgct gttaaaagta caggtgtact tacttttatt
    30181 aattcatatc catcttctaa ttgtctttga actattatat cttttccttc tattttaaaa
    30241 tcttgtacat atgtaacttg aggaatgtct aatttctctg caatttgtgg tccaacttgt
    30301 gcagtatctc catctattgc ttgtcttcct gcaaatatta tgtcatagtc acctatttta
    30361 gatattcctg ctgcaatagt atttgatgta gcccaagtat cagctcctcc aaatgctcta
    30421 tcacttaata atattgcatc atcagcaccc atagctaaac actctcttag cataaagtct
    30481 gcttgaggag gtcccattgt tataactgtt atagttacat catcatactc atctttaatt
    30541 tttaaagctt cctctaatgc atttgcatca tctgggttta atatacttgg aactccatct
    30601 cttataagag tacctgtttc tttatttatt cttacttcgt tagtatctgg aacttgtttt
    30661 acgcaaacta atattttcaa agtctatacc tccaatttca ttagattatt tattatttta
    30721 atacgtttga tgaaattacc attttttgaa cttctgatgt tccttcgtat attgatacga
    30781 ttctagcatc tctgtacatt ctttcgattt cataatcttt tataaatcca tatccaccgt
    30841 gaagttgtaa tgctttataa gcaacttcat ttgcaatttc tgatgcatag tatttagcca
    30901 tagcagattc ttgagcaact ctcattcctg catctctctt ttgtgctgca tcataaacta
    30961 atcctctagc tgcacaaact tttgtttcca tgtcagctat agtaaattga gtattttgga
    31021 atttagctat aggcttacca aattgaactc tttgttttgt atatttaaca gcttcatcta
    31081 aagcaccttg agctattcct aaagctaagg cagcaacacc tattctacca acttctagag
    31141 tacccattgc tatcttaaat ccttgacctt cttttccaag taagttttct tttggaactt
    31201 taacgttttc aaatattaag tcagaagttt cagttcctct tatacccatc ttatcttcat
    31261 gagctcctgt tgagaaacca tcccatttac tttctactat aaacgctgat gttcctctaa
    31321 gtcctttact tctatctgta actgctatta ctatagctac atcacagaat ggtccattag
    31381 ttatgaaagt ttttctacca tttaatatat agtggtctcc aactaattct gctgttgttt
    31441 gttgtcctgc agcatcagaa ccagctcctg gctcagttaa accaaatgct cctattaatt
    31501 cacctgttgc tatacctttt aagtattttt gtttttgctc ttcagtacca ttgtaaagta
    31561 ttggtaatga agcaagagaa gaaccagctg taacaaaagt agctgttgaa gcacatcttc
    31621 tagctatttc ttccattact atagcgtaag aaacatatcc tgctccccca ccaccatact
    31681 cttctgggat attactgctt ataaattttg catcagctaa ttttttgatt aattccttag
    31741 gcattacacc tgttttatcc atttctgctg gtaaagtatc taattcttta ctaacaaaat
    31801 ctcttactgc ttttcttaaa agttcttgtt ctttgttata taacatagct tcctccaatt
    31861 ttcatatttt atagtaaatt tattttaatg aacttacgct aaactcatca tctcagcaaa
    31921 agtttgaata cgagttctgg cttgttcatt attctgagtt tgttggtcga tttcaacata
    31981 taaagtaggt attccactat cttctatatc ttttctaact aaaggataat cgtattcttc
    32041 tgggtcacag aatttcatca tacagaagat aacaccatct atatctttct ttttaacttc
    32101 atctactata agtgacccac gttttttctt agggtcataa gctaatgaac atccttctat
    32161 gtttgaccat tgtcttgcta atctttccaa cgcatcatca cctgctggta catctgttct
    32221 gaattgtctt gtttcttgtg ctaagtcatc agctacaact gatatattgt tgtcttctaa
    32281 aatatctaat atatctttag aatcagctaa tatacctgtt aataaaactt tctttccaga
    32341 acagacttct tctggcatag catttaattt tgctattaaa tctttcacta gtttagtgtg
    32401 ttcttctttt ctcataaaga atccactctt aattactaag cttcttattg atggttttat
    32461 agtattagaa tgtttataag ctacttcaac aaagtctctc atagtttttc tatgttcatt
    32521 gtaaacttct atactttcat gaatttttgc ttcttctatt tcatatccac atatttcttc
    32581 aagctctctt tttacgcctt tgtactcact gattaagtat tttacaccag cttcaagttt
    32641 tctattttgt gggtgtacta atgatatata ttttatatga ggtactgctg atttccagtt
    32701 ttgtcctaaa caaattaatg tatcacacat acctggtata ataactccag ataattcatc
    32761 ataagcacct tttaatccat attctaaaca tgattgcatt attgaacatg caaatgcagg
    32821 gaaatattgt ttagctaaat ctaattctgt ttgtcctccc catataccaa ctggaagcat
    32881 tccagctgca tgtataattt cttctgggca ataaactggg aaacaaccta tagctttttt
    32941 accagtttca cttttatatt tttttacagc cgcatttgga ttttcaacta cttctttcat
    33001 tttagataaa atagcttcca ttatatctca cctctattaa gtttttttct ttcttccatt
    33061 acttcaacta aaccttgaat tcttgtttca aattgagcat tagtgaaagc tctagggtct
    33121 gcttggtcac catcaaatcc agcatatggt aatccagttt cttcagctgc tcttctttgc
    33181 atttcatatt gtattaaact cataagttta caacttctgt tcatatgata aaatgctcca
    33241 tcacatttac cctctattaa agaatcaact ctatattttg tcatacggtc tagatttaca
    33301 ttgttaaaca tagtactata tgctactgcc attccatcta aatcattaac ttcatattgt
    33361 aatgcccaag catgtgggta aacactacct gtcatgttaa ctccaaattt agctaatgtt
    33421 ttcatcttgt atcctatata tggccaacaa ggtatacctt ccatcattat tctgtatttt
    33481 tcttctcctc tgaaagaaga tttaccagtt ttcatgttgt cctctaattc ttctataagt
    33541 aacttaaatg cttctgtagt ctctttttta cctctagcac aaactattac agccatataa
    33601 gtaaataagt caaatccatt cattggagaa ggagaagaat ctgctggtaa actcatagaa
    33661 tacttccata gtcttccgtt ttcagctgat attttcatta cctcttcaaa tttcttaggg
    33721 tcaaatttct ttcctgatat aatttctagt tgttttatag cttcttcaaa ttgagcttta
    33781 atataatcta ttcttgattg agtaacttca tcttcattat tgaaagttgt atcaatcatt
    33841 attaaaggta tatctaattc tcttgaaata ttttcatacc attttataac ttggttacat
    33901 atattgttac agcaaagtag gaaatctgga gctggcatat ccaaagcttc acatccacca
    33961 ttttctaaaa gaccaaaatt tgttcttgca tatgcacata ggtcaataga atatccttta
    34021 gattcagcca tttcacataa ttctaaagaa ccttttttag ctgctactcc agccgcttgg
    34081 ttttctggat ataatacgtt taagtcaaat acttctgcta attcttgagg aaatactgat
    34141 gttgaccaac ctacaggtct tccttcttct ttagctttaa atgcatttgc atattgttca
    34201 gctaataaat cattaattac tactctagct tctttttttt cagacatttt catccctctt
    34261 tttatatatt tttcacttct ttttgtgatt cttttgcttc atcaaaagca tataacgctg
    34321 ctcctaaagc accagttaat tgaggtatat caggtacaat aatttctgtg ttgatttctc
    34381 ttgccatagc tcttacaata ccactatttc tagcaactcc accaaccata actacatttc
    34441 tttgtactcc tattcttttt actaggctag aaactctctt tgctactgaa gtatgaatac
    34501 ctgctactat atcttcaatt tttgcatttt cagataaatg tgatataacc tcagactctg
    34561 caaatactgt acatgtactg cttattgata cttcattttg agaattcata gatatacttc
    34621 cgagttcaga tacatcaacc tctattattt ttgccattac atctaaaaat cttcctgtac
    34681 ctgcagcaca tttgtcattc ataagaaagt ttagtagtct tccattatta tctaacttta
    34741 atacctttgc atcttgacca cctatgtcaa taatggttct tgtctctgga attatgaaat
    34801 ttactcccct agcatgacag cttaattcac ttatttgctt atcagcatct gaatagttca
    34861 ttcttccata tcctgtaact acaacttttt taatatcttc tcttgcaaga cctgtcttgc
    34921 catataattt ttctaaaact cttgatggtc cagtagtccc agtaccagag gatattgttt
    34981 cagaagctac aatatcttct ccattcttta agattactcc ctttgatgca gttgaaccta
    35041 tatctaatcc cattgtgtac atctttagca ttctcctttt aaaaaatcta gaattactaa
    35101 ttaatatctt acaactttac tatctttaaa gttatttatt ttctcttcat catagcctaa
    35161 agattttaat acttctacag tatgttgacc tacttttggt gctggtgtat attctttaat
    35221 tccttcattt ctaaacataa ctggagtatt aactaagaca cctgtatttc cgctatcata
    35281 tgttttctta aataagaagt catttgccca agcttgttcg tcatctaata aatcttcaca
    35341 gctttgaatt ttttcaaatg gtaaatctgc ttcttctaat aaagctgacc actcgtctag
    35401 tgttttttct aacatagctt ctccaactat tttaactaaa tcttcaacat gattaaccat
    35461 tgaatctatg ttattgtatc tatcatcttc taatatatat tctctattta taaccttaca
    35521 gaatttgcct aaccacttgt tgtattgtat taaagctaat tgaatccatc ttccatcttt
    35581 acatttatat gtagtcatta atgggctgtt tggattttct cttgataaag gcatttcatt
    35641 tccgtattgt gctgttgtta tcattgttcc cattccatat atagctgtat ggaaaagact
    35701 tactgttact ctctcacctt taccagtttg acctttttta tgtaatgctg ctaaacttcc
    35761 tgccgctagt gctagacctg cataatggtc accaaatcct gctgctgtat ttgctggaga
    35821 tgttcctttt tccataacag attggctaac tcctcctctt gcgaagtatg cagtatagtc
    35881 aaatcctggt ttatctttta aaggtccttt ttcaccatat cctaatattt gagagaatat
    35941 taatcctgga tacttatctt ttatttggtc ataagctata cccatttttt ctaatgcttg
    36001 aactctaaca ttagttacaa atatgtctgc ttctgataat aatttatgta atatttctac
    36061 tccttctttt gattttacat taatacttac acccttttta tttccatttt ctaattcaaa
    36121 catagggttt tcatcatctg atgctggaga tttaaatgtt ccacccataa ctcttattcc
    36181 atcaccttct ataggttcaa tcttaataac ctctgcaccc cagtcaccta acatttttgc
    36241 acaacatggt gctgcaatga aacttgaaag ttctactact ttaactcctt ctaaaagcat
    36301 aacgttttcc tccttaatag tatcaattat aaaatttcaa ttaaatcact cataaaatac
    36361 tttttctgat attaactgtt tgatgtcaaa gttcctaatc ttatctaaaa taacttaaaa
    36421 attattaaaa ttatttttta ttgttttatt gtcttacacc tatattgtta agcatttatc
    36481 gtgccaactt tatatttaat caaaatatcg cttgatttga atgtttttta acaatatcca
    36541 atcaaataat ctttttcata tcgtggcatc ttgcaactat ccattgcatt ttgctattct
    36601 ttctatctct ataaaagttt tttttatata tttttttatg aaaaagcttg acatttcaaa
    36661 aaaagttttc attgctaaac tcaaaaacac atgttatcat tgtaattaat cagcaagtta
    36721 gttaactaat ttcaaactta gataattttt taataatatt taatctaaaa aataacaatt
    36781 tctttgataa atttttagag taattattac attaaataaa aaattactaa atatcaaaat
    36841 aacacaggga aaacaatttc aaagctaaat ttttaacctt aaaaattttg tataaaaaat
    36901 atagccttat ttaatacata ttattattta atacatatta tttctaaatg aaaaattacc
    36961 ataataattt aataattaca ggagggtatt gatatgaaaa tactagtatt tggagcacgc
    37021 gattatgaag aaccagtaat aaaaaaatgg tctgaagaac ataaggatgt tcaagtggat
    37081 atttatcctg aaaacatgac tgaagaaaat atagttaaag ctaaaggata tgatggtata
    37141 tctatacaac aaactaacta tatagataat ccttatattt atgaaacttt aaaagatgct
    37201 ggggttaaag ttatagcttc aagaactgca ggggttgaca tgatacattt tgatttagtt
    37261 aatgaaaatg gacttattgt tacaaacgtt ccttcttatt cacctaatgc aatagctgaa
    37321 ttagctgtta ctcaagctat gaacctttta agaaagactc ctctagtaaa gaaaaaagtc
    37381 tgtgaaggtg attaccgttg gatagctgaa cttcttggaa cagaagttag atctattaca
    37441 gttggtgtta taggtacagg aaagataggt gctacttctg caaaattatt caaaggccta
    37501 ggagctaatg taattgcatt tgaccaatat ccaaatagtg atttaaacga tatattaact
    37561 tataaagatt ctttagaaga ccttctaaaa gaagctgacc ttataacatt acatactcct
    37621 ttacttgaag gaacaaaaca tatgataaat aaagatactc tagctataat gaaggatgga
    37681 gcttatatag taaatactgg tcgtggtggt ttaattaaga cagaggactt aatagaagca
    37741 ctagaatcag gaaaaattag agctgctgcc cttgatacat ttgaaactga aggattattc
    37801 ttaaataaaa aaatgaatcc aggagaatta actgacccag aaataaataa acttctttct
    37861 atggaacaag ttatattcac tcatcacctt ggtttcttca ctagtacagc gattgaaaat
    37921 atagtttatt ctagtttaag tagtgctgta gaagttataa aaacaggaac tgctactaat
    37981 agagtaaatt agtattttat aaatatattt tttcattaat tagctattat ctaacaaaat
    38041 agcctttaat ttcccaagta tgagagagtc agctcaatat atattgagta gactctctta
    38101 tattattttt tatattaaac ttatactctt cttattcata ccaaattaca tttatattaa
    38161 tttattctaa ctactcttac tctacatata ttttacccat ttttactttt ctaatttttt
    38221 ctaattatat acatttgaat gggtacaatc tgtattttac aaaaaaaaca cttaacaaac
    38281 tatgaaaatt gtatgattaa tattaatata tttataagaa aacattacaa ttatgttagc
    38341 tctggatttt tagggaggga gtatattgga aaaaatgaat aaagttactt ctaaaaaaac
    38401 tacctatatt gttacttcag cattatttgc atccataata tgtctgacaa tagcatatat
    38461 actacatatc ccagttggtg gaaacaatgg atatgtccat ataggagatg cttttattta
    38521 tctagctgca actattttgc caacaaacta tgctatagct gcttctgcaa ttggtgctgg
    38581 tctagctgat ttatctacag gtgctgctat ctgggtaata ccaactataa tcataaaacc
    38641 aatacttgta ttatttttca cttctaaaag tgacaagata ataaataaga gaaatatagt
    38701 tgcttcgatt gtagcaggta taataggatt agttttatac atgtttgctg aaggtataat
    38761 tattggaagt tttacaagtg catttgtaat gagtttactt ggcttattac agccaatagg
    38821 aagttttatt gtttttataa tcttaggtat ggcacttgat aaacttgatt ttaaaaaacg
    38881 ttattttaat taagaattta aatttatgga gggaaaatta tgaatatctt atatacaatt
    38941 gaaaatagta tctatgtgaa tataactaat acatgtccat gtagttgtgt tttttgtata
    39001 agaaatgaaa aagatgaagt tgcaaatagt ggtagtttat ggttggagca tgaaccaagt
    39061 gtagacgaag taaaagaagc cttcaataaa tacaatttag acgattatga tgaaatcgta
    39121 ttttgtggat atggcgaacc attaatgaga ataaatgaat tgatagaagt agcaatgttt
    39181 ataaaagaaa aaagtagcat aaagataaga ataaatacaa atggtctaag tgatttaata
    39241 cataacaaaa aaacagctat tatgttaaaa gatgttatag atgcagtatc aataagtctt
    39301 aatgctccaa ataaagaagc ttataataga gttactcaac caaagtttgg agaaaaatca
    39361 tttgattata tgttagattt tgcaagagat tgcaaaaaat atataaaaga agtggctttt
    39421 tcagtagtag atgaaatatc tccaaaagaa atagaagaat ctaaacaact agcaaaaaaa
    39481 cttgatatac cattaagagt tagacataag aactagcaag taaaattgta aaacattaat
    39541 aatcacctga aaggtgtttc aggtgattat ttctcttgta ttaacatttc acaaaataga
    39601 tttcatacca cactaaaaat gtgtaaatcg tccaaatctt tctccaatta ttagaatttc
    39661 cactaatgta gtcattaaaa atctcattaa tctcatctat gttaaagaat ttatctgcta
    39721 tctcactatt gaatttatct cggatatcct gattatattt gtcatcagcc atccatatac
    39781 ggattggtac aataaatcct aatttattcc taaaagcaat ctcttctggt aatacttttg
    39841 atgcagcttt acgaaaggca attttattct ctccaccttt cactttatat tctgatggca
    39901 ttcttgatgc aatatcaaag attcttgtat ctgtaagggg cattctgact tccaatccaa
    39961 ctgcagtact cattttatct atattaaaat aaatgtctcc ttccaaccat atttgaatat
    40021 ctatatttga catattagta agtgtatcca gttcctctgt ttcttcataa attctttttg
    40081 tcagtttaat aggaagcaca tcaggattgt atttcttaag aatttttcgc ttctcatctt
    40141 ccttcataat gttcgtattc ccaatataaa atgtctttag attctcccca taacactttg
    40201 ccttatgata tacattatat ccaccaaaga actcatccgc tccttctcca gagtaacaaa
    40261 tatttgtgtt ctcagctgct gccttgcatc caatagcaaa aacaatagca gatgcatctc
    40321 cagttggttg ctccatatgg tacattacat aaggtactga cgcaaaatac tcttcaggtg
    40381 taattatata tctcgaattt ttacatccaa gaatatcagt tgtctgttgt gctaattttg
    40441 attcatcaaa ccactcttcc tcataaccac atgagcctgt tttttctata tctgacattg
    40501 ccaatatata agatgaatca acacctcctg ataggaataa ctctgcagtt tcatcctctg
    40561 attttatctc tggcataatt ttttgaagtg ttgtatgaat ttcatctgtc cattcctcaa
    40621 tggacttgct gtagtcagga tgaaattcag gtttccaata acgctcaata catactgttt
    40681 tgttctgata gacaaggtaa tgtcctggca taagcttttt tactccacgg aagaatgtat
    40741 tttcacctgc tacatatgta aaagacatat acatctgtaa catttcttca tttaactctt
    40801 tcacaaatcc tggttgttcc ataagcttac gaatagatga actatagaga agcttcccat
    40861 tttcagtctt ataataatag aaaggtttta ctccaaactg gtctcttagg caaaaaagct
    40921 tcttttccac tgtatcccat agtgcaaaag caaacattcc atgcatgtga tttgccatat
    40981 catatcccca ggttttgtat gcttctacta aaattgctcg ctcacgctca tctctaccta
    41041 aagaagaatc gattcctagc tcctcacaga gaactttcca atttctaata tgtccattgt
    41101 attctaaaat ttttatcata catgtatctt ttcgtgttga atacttttct tctaagttta
    41161 tcatatgtgt atcctttcat attaaaacat ttaattctta aatttattga ttgtttttat
    41221 ttatatttat tcacttataa tcattgaaaa tctacttttt tcaaagtaaa ctctttttta
    41281 attagtgcta atatgctctt tgttaaggtc accaatatga ttattaatta ttatcagtgt
    41341 tataacattg aattttttat ttttcataaa tattgccctc ctttaaatta ttagctaaat
    41401 ataatatatc ttatgttact tacatattct ttaagaaatg ctgaagattt tataaagttt
    41461 tctgtagtat aattaattaa aaatacaatt ctagttataa agcatatttt ctattaaaca
    41521 aaaaatagtt aaaataaatt cataataaca taaacttatt ttaactattt gtatatttta
    41581 gttataaata ttgacttaaa tttttaacag gatacactca taaaataaac acgataaaca
    41641 ataaaataat atatcttagt ttctttatat actttgtctt ctttcaattt tctaccatgt
    41701 agacggttaa gataatatta gtaattcact ctactgctta actcgtgcga tatggacaac
    41761 caagaaagct atttcctcat ttgttaactt ttttccatat ttcttttcta aaaaatctct
    41821 aacttttaat gcaattgaca tttcttttgg atttgacctt ttcatttgat tgaataataa
    41881 atcatctaca ctacttaaca tgctatctga aaaaaatctt tccacaaaaa attttatatg
    41941 tgttataaat ctcatataat gcatactttt tttatctaag gttctattca aagaaaaaac
    42001 aaaaatatta attatttctc caattacttt ggcatattta atcgtatcat atgaatttcc
    42061 atctgaagcc atagcgtttg caaaatgaaa agcaatattg gctgcttctt cttctggtaa
    42121 ttgaatgccc aatctctctt ctaccatgta aattgcaacc attccaacct taaactcatt
    42181 tggataataa ttttttattt cccaaaatac tcgatttgta atttttattc ctttacgcat
    42241 cctttcaatt gcaaagttaa aatggtctgc taatattaag tataagtttt tattaaaaga
    42301 tgagttcaat aatttttctg cctctactat tatttcctgt gttatcttta aaatgtcgac
    42361 agaaatatca tccattaatt ctaaaaattg ttttgatttt gtattttcaa cagggacaaa
    42421 catttgatta tcattggaac cacaaatata ttctcctgtt tttttaccat atccaattcc
    42481 tttccctagc aaaataaatt cattatcatt tgcatcatga actaaaacaa cgctcgagtt
    42541 caaaactttc tttattatgt agttcatttt atttgctcac cttctattgg tcatttaaaa
    42601 tctatactat tattttcaat tactttttta taccaataaa aactatcttt cttatatcga
    42661 ttatatgtcc cctttccata atcatcagca tccacataaa tatatccata gcgttttgac
    42721 atttgtttcg ttgattcact gaccaaatcg atacaacccc atgaagtgta tccccaaagt
    42781 tcaacaccat cttcaataat cgcatctaac atacatttaa agtgttcttt taaataatca
    42841 attctatatt ggtcatggac ttttttatct tcagttaaaa tatctttcgc tcctaatcca
    42901 ttttcaacta taaataaagg ctttctgtat ctatcatata attcaatcaa agatatgcgt
    42961 agaccaattg aatcaatttg ccaaccccac tcactcattt ctaaataagg attttttaca
    43021 gctaataacg tattccctga tgtaacatct aatccagttg tatcggctgc ttcacaagaa
    43081 gacatatagt aagagaatga aataaaatct actggatact ttttcataat ttcatcatcc
    43141 tgttcttctt ttactatttg aatgttatta tttttaaaat atgacaataa atatactgga
    43201 tactcaccaa aaacctgcgt atcactgtat gcataaatac ttctcatttt ttgttgggct
    43261 gccaaaacat cttctggtct acatgtatat ggataatatg ttaattttgt taacatacac
    43321 cctacttttg aatttggaat aatctcatgg caaatttttg ttgcaagagc actagctaca
    43381 aattgatgat gcatagcttg aaaaataact ccctcaaagt ttttatcctt aaaacggtct
    43441 cgaaccaaac caccagtagt gtatggatgg cgaatcatag aatcaacttc attaaatgta
    43501 agccaatact tcactttgtt tttatatctt tcacagatag tttctacata cctaacaaac
    43561 atattgataa cttgtcgtga ataccatcca tcatagttta aaacgatatt taatggtggt
    43621 tcataatgtg acatagtaac aagtggttca attccataac ttattaactc atcaaataca
    43681 tcatcataaa actgtaaacc ttcttcattt ggttctgtat catctccatt tggaaaaata
    43741 cgagaccaag caatactcat tcgatatgtc ttaaatccca tttctgccat cagagcaata
    43801 tcttctttat agtgatgata aaaatcacta ccgtgacgtt ttggataatt tacttcatct
    43861 tcaggatgtg ctaatccttc ttctatgtct cttgtcgtaa tttcattatt ggatttatag
    43921 tctttcacat ccacatcaaa gtgtgctttt gctacatctg atacagacca tcctttacta
    43981 cctaaattcc aaccaccttc aaattgattg gcagctgtag ctcctcccca aaggaatcct
    44041 tctggaaatt ttctcatact tatattcctc cctttacatc aaacaagatg cttccttttg
    44101 ttacattgcc ataagactga tttaacactt tatagtcata atctgcccca ttactattta
    44161 caatcataac agttgaatct aaattagcat gtgagatttt gtctaaatca aactgaatta
    44221 ataaatctcc ttcttttaca ttatcgcctg tcttaacaaa tgttttaaat ccatctccat
    44281 tcatacttac agtatttatt ccaatatgaa tcaaaatctc agccccattt tttgttctta
    44341 aaccaattgc atgttttgtt tcaaatagca tcacaacttc tgaatcacaa ggtgcgtata
    44401 aatttccatt ggtcggtata attgcaactc cttctcccaa agtcttactt gcaaataatt
    44461 cgtcactaac tttacttaaa tctatcactt ttccttctac tggagatact aatatattta
    44521 cttcttttcc ttctattggc tcttcttcaa caacattttc aattgatgac tgttctatat
    44581 ttgtatcttc cttccaaata attagtgtaa taataaacga acctaatatt gcaataacaa
    44641 aaccaatgct tgcaaaaatt aaattatttc cattgtttgg gtcaataaac atgctcatac
    44701 tagcaagccc tggtccaaca attacaaatc cttttactgt aaataaccct aaaaatatac
    44761 cagaaatcgc acttgacaat acaacaccta gtattgctct tttatgttgt aaagtaatac
    44821 cgtataaagc tggctctgta attcccatca atgcagaaat tcctgcagat aatgctgttt
    44881 gttttaatgt ttcatctttt gttcgcaatg ctactgcaaa acaaacacca ctttcagaaa
    44941 tgttgtgagc taatgaagct gtcatatata atgcttcata acccaacttt ccaattgtat
    45001 taattgcata aggtgttaat gctttgtgca ttcctgttgc aaccataaat ggaagaacag
    45061 cagcaagtaa tcctacaact aagaatccca ttttgttata taggaataaa ataatggttg
    45121 taaaaattgt cccaacatta tatcctaaag gacctaaaat agttagagta attggaacag
    45181 taattgccaa agacatcata ggaacaaaga aagttcgaat tggttttggt gaatacttgt
    45241 taaaaatttt ctccattatt gacaagaaag acacacataa aatagctggg aaaacctgtg
    45301 cattataggc aatacttgga atcgtaaatc caaataattc gacaccttca cttaaagcag
    45361 tcgtcgttgc tggaagtaat aagtatccaa cagcagcaat agctactaaa cgattacatt
    45421 tcaatacatt ggcagtagtt acagccacca tgagaggtaa gaaatagaat gttgcatctg
    45481 aaatagcaat taacaatgta tatatagtag aatctgattt taaaatatta aacattgcta
    45541 ataacattaa cagtgatttt agaataccag ctcctgcaat agcgaaaact aatggttgaa
    45601 aaatattaat aataaattct atgaatcttg ttcctaaatt tattttttct tcagttttta
    45661 tagcactatc atttgtatct aaagggcaaa cttttaaaat ttcatcataa acatcaacaa
    45721 cactatttcc aatgacaact tgaaattgtg ccctatcaac tatactcata acaccttgta
    45781 ctttttttaa agcatcttga ttgactttac ttctatcaat caacttaaag cgtagtctag
    45841 ttgaacagtg ttccaaagct agaatatttt ttgagccacc aacttgttca ataatgtcaa
    45901 tggcagtttt tttataatcc atcttgtaat ctccttctcc ataatttttt atgaatggcc
    45961 attcaagatg caaaaaaggc agaacctata taaacacctt gaaaaatcaa agcaatatat
    46021 tcgatcctgc ctgcattacc agtaacatgt cttctattta tactattttg tactaccata
    46081 ttaacatttg cctaaaagaa ttgtcaatac ttttttaaat tttctgatac taactaatag
    46141 cgcctttgta attcattttt catatcaatt aaagtaacag tagccaccca ccaattagta
    46201 tcaaaattga acctggtatt ttttctttta tattttcttt gaatataaaa tgagcaagta
    46261 ttaaagttat tatcatagat acttgagaaa atggttcagc tatactaagt tctagattat
    46321 ttaaagcaat taataacgct aaataagagt atccattaat agcaccactt aaaattgaaa
    46381 gccctttttt attattaaaa atctcccaaa ttactttttg tttcttttta aatgctaaaa
    46441 atataaataa atttaaacct acaaaaaaat acagtatagt tgaatataat ataggagata
    46501 cttgtacaaa aaaagctttg tccaatactc ttcctactga ctgtaaaaat atgtacaaaa
    46561 acattaatct acatggtaaa ttgttaaaaa ttacagagac attctttaca aaggagctta
    46621 ctttttttaa tataaacact ccaccaatca taattattat tcctgcaact tttgctaaac
    46681 ttaaaccttc acctaaaaat ataactgata aaagtagtaa aaacaatgaa tttaaactgt
    46741 ttattggagt aacaagtgaa acttctccag ttgctaaaga tgtcacatat gctactgagc
    46801 caattgaata tactaaacta cttatataac atggtattaa aaaagaccaa tcacttactt
    46861 tatccattat actaaatggc agtaaacata ctcctcctat aaagaaaaat aaaaatgatg
    46921 aagctatatt tttataaata tctccttctt catcacccaa taatcttaca actactcttt
    46981 caaaagctaa aagcactact ctaattaata atgcaaaata agataatatc atttttgacc
    47041 tcgcttaaaa attaatctga cttattgtta tattgattgt acaaaactac catataggta
    47101 aatcttttgg attttttaat attttatgct taaatacagg tatattaagt ttttcaccta
    47161 acttacggca ttgctcctta taagtacctt ttaacttatc atgaaaaact atgccatcaa
    47221 tcccatctgc aatactattt atacactttt ctagtttctc aagtcccgat tcatcatcat
    47281 acattccact tacaatccat tgatttggca atacatgccc aattgatatg ttaaattctt
    47341 tattttttat aatatctgag aaagatttct caaaatgtac ccctccaaca cataataaag
    47401 tcttcaaatg ttcatcacaa tcaaatactc taattaatga ttttgccaat acctgtattg
    47461 ctatactatt attaaaacta tcactactac ttcctatctc aatgtccaac ataggtacag
    47521 aatattctgt aattaattta gattctccac catatgttgt tccagaccag tgggttgctt
    47581 ctgttaatgt agtaaattca tcaagtgaat tttcattttt tatatcttca atagcattaa
    47641 ttaggttttt aaagtatctt ggatttgaat tgccaaattc tcctgttgga acatctccaa
    47701 ttgtatgagc tgttaggata tgttctggag cattttctcc ctcatgccaa tttacaactc
    47761 ctgcaaaatc ataatctctg aaatgtctat ttaaaattgg aagataatat tcataatcat
    47821 gacttattac atattttgtt tttacaaaat gaaataagtt attgttttta tctttaaact
    47881 ctaatacaaa attatcatca ataacaatat cagtttgatt taaatccatt atttcctcac
    47941 aacactttat tacattacta gctactgggt ctttctttaa atcactacaa aagaaaaaaa
    48001 ctgctttttt attattcatc cataaatcac actcctataa cttaaatata atctgttctc
    48061 atacttaaaa ttatttaaat atcactctag ctccattaaa ttatttatac ataatatact
    48121 accatactca ttttagctat gcctttgcct gagtttctat atatatgtga tacatcagct
    48181 ttaaatctta tagaatttcc actagataca acataatctt ctccatttat tttaatggtt
    48241 acctcaccac taaaaacagt tataaactct tgtgttcctg gaagttcagc atttgaatct
    48301 aaatttccac cctcctctat ttcaatcata aatatctcaa accttctttt cgaatcaaat
    48361 ggaaatattg gatagcttat atattttcca tcatcttcaa taaaatggtc tacatcactt
    48421 ttttgaatca attctatatc actttcctgt ctctccatca atgatgtaaa tgagattttt
    48481 agtccattag ttattttctt caaagtagat atggtaggat ttacctcaca cttttcaatt
    48541 tgagacagca tactcttact tacaccacta agttgtgcaa tcttatctaa gctaagatgt
    48601 ttttctttcc ttatcttttt taagttctca gcaattattt catttaaact ttgcataaat
    48661 tcctcctaat atgcttaata aaaacaagtt attttgatta tttaatagat ttagtaactt
    48721 aatacaattt ttatccatta taatgtacat tatgtttaat atacattact tctttaagaa
    48781 tgtaaatagt tttctgaaat tttgtaaaag tggacaaata aaaagctagc aacctatatt
    48841 tttacaaggc tactagcttt tattatcata atatttcatt ttaactataa aacttagatg
    48901 cacctataag ctgtctctta gaaacaatct tagctaagtt tgctaatgaa gaaattttta
    48961 tatctttaaa agaaactaaa aatacaaact taaaatctgt aaattcttga tttatattgt
    49021 atatatcaaa ataacatctc aaattatcaa ataagtaatt atcttgtaaa tctgcttcta
    49081 tctcatcagt agcatctttt aaatacgctc ttacgtcttc aaataaaaca ctgaaataag
    49141 attcatctat ttcttcacct aattcttcta atatttcttc tatatctaaa tcgttcatat
    49201 caaaagaatt tcctgtcaac tgacaatcaa atactaatat attttcttga tttatagtat
    49261 ttgatatatc ttgtacatat tcaaaatctg gacgtaattc taaaatgtct tttatttcac
    49321 tactctttac atggattttt ccttttctat tttcaatttt taaatctatc atatgctccc
    49381 cctttcaatc tacaaatctt taaatacctt gttttataat cttttcaatg tctatccaat
    49441 tattaactct aactatattt tgatgtttaa catctttatt atagttagta tccagaagta
    49501 ttactttaac accatcatca gctagttgca ctgagtttaa agggttatct tctataaata
    49561 tgtcacaact cagctctctc gccttttcaa ctttataatc acttcctaat aagtaaacat
    49621 ctatatctga aaatccttgt ctcgataacc aatttttagt tacatcagtt aaacactcat
    49681 gtctagctgt tacaaaagat aaattatgtc ctttagataa tgtgctaatt acctctgtag
    49741 caccctctac aatctttgct tcatcatatg aatgtatata cttattatta aactctgcat
    49801 atacatccct tattcccgaa ccatacaatt cttcccaatt atgtgttata cactctgctt
    49861 cagtgatatt tttattaaaa atatcattta ggtatggaag aaaatgataa gggcttgtta
    49921 ttgtcccatc tatatcaata catatattca atttgttcat ttttacctcc atgtaacatt
    49981 tattgatttg attaatcaaa cattatagca cagctttaat tataaatcaa aactctagat
    50041 atgactatac ctatataata ccaaaaagcc tgcatattta agcaagcttt tcgtaaattt
    50101 tacattttta actaatatat aagtttttct aaggttggtt ctactccaaa ttgtgatttc
    50161 agttctttat attcttttaa tgctttatca ttatttgttc tagattttat agctcttatc
    50221 attaaatttt ttggtgtgtc aagtggagat atgtattcta ctacagaagt atcatatcca
    50281 ttgccctcca ataacaatgt tcttaaacca tcagttatta agtctgcaaa tctagcttta
    50341 aatacaccat gttttaatat tggttccata gcttcatatc tatattgacc caataactcc
    50401 ttatgacaac atggcactac tactatagct tcagattttg ctctaattcc taaacctatt
    50461 gccatatctg tagctgtatc gcaagcatgt agacttatta caaggtcaac atccctatta
    50521 ggtgtataat tagtcaaatc ctcttttata aatgacatat ttttataacc taaattttta
    50581 gccatatttc ttgatgcttc tataactaca tctgaataat ctatacctat aaaataacaa
    50641 tttttcttca atacctcttt tatataaaag tttaagacaa agcttaaata agattttcca
    50701 caagcacaat ccaagatagt tatacagtcc ttatccttta tttcttttaa gatattatct
    50761 atcaattcaa caaaatggtc tatctgattg tattttctta ttttatcatt ttttatctta
    50821 ccatcttttg taagcactcc aatttctttt aaaagtgcat ttgcctgtcc tacttttaca
    50881 taataatctc tgttttttat ttgagatgct gtaaattcat ctattttagg tacttcttcc
    50941 ttattatcta aatattttac atttaccttc ttattgtctc cttcaattaa aacaatagtt
    51001 cctcgctcaa catatttaaa cactaatcca tcataatttt ctgcctcttt agaaatattc
    51061 ttaagcaatt cattaaatgt atataaatct gattttcctt taaaattaaa tttatattta
    51121 ttatcttcct ctattgcaat tcctttaaac tcttttcttc cagatttaaa aattacctct
    51181 aactcaataa aaaaatccct gttatcaaga tttctttgag taatccccat aaaaaacata
    51241 tttagttttc ctatactctg cttattcatt gtaatctccc ttttaactta tatccttaaa
    51301 tcttattaat tgatactatt tgtttctgtg ttatttattt cttcttttaa atcattcaat
    51361 ctatttttaa aactttctat ctgcttatct gataaatcaa gatattctgt tatcttccct
    51421 atatacttag ggcttttagt atttattaat ttcaaagcac tttccataga tttttctcta
    51481 agttcatatg ctttctttac atcatctcta gcattactca atgtttctgt atacttctcc
    51541 ttagaaagtt ctggaatctg catattttca tattctttta caacttcttt acacttattt
    51601 cttgcatttg taacatcttt ttttatacta gataatttag ttatattttt tgcgatatcc
    51661 ttagtgtaag gtatacattc tctcattgga actaaagctg ctctttctat attataaatt
    51721 tctttcttaa aaattcctat actttccata tctatttttt ttatatcttc actatttttt
    51781 tctttacttg tatatttatt attttctttt gtagtatctt cttgtttctc tgatttatta
    51841 ttcactgaaa cattactatc agagttattc ttttctttta tgttattact tccttcttcg
    51901 ttgctttctt tattttgtat atttttattt tgtttgttgt ttaaattttt attatttgat
    51961 atctcttcat tctttttctt atcctgtttt aaatcagtac tttctttttc cttaaagttt
    52021 gatacatact tttcgtcata tgtctcatca gaaatataat tgtgataata catagattct
    52081 tttattttat taagtccact catataaata ttacttatca ttattgttgt agccaatact
    52141 acataaatac tactatagat acaagacctc ttaaaattct tgtaatttct gaatatcact
    52201 actaaaggaa taagatataa aaatataaaa actaatatcc acctcataag tcgtgtctcc
    52261 tttctcaaaa ccgaaatccc tcaaatattt atttttaatc atgtatctta tagtatttac
    52321 caatttatgt tttttaatcg tgaactttta ttaaattaaa ttctgtatag tcacaagatg
    52381 ctaatgtata ttctactcca tttcgaatcc ctttcaatcc cattttaaaa gattctttac
    52441 catgcaagtg accataaatc accttttcaa cattatattc ttcaaataac tttgtaaata
    52501 aagattcttc taatttatca tttgtaggag gataatgagt aatcacaatt atttttgagt
    52561 gaccactttt acgagcagat tctaatgata acctaagtct atgttcttct cttttataaa
    52621 ctttttcatc agtctcatca aacttaacat cattaggaca tatccagccc ctacctccac
    52681 aaattgcata atctttatat tcataaaaat ttgtctgaat aaaacgcata ttttcatata
    52741 acttatttaa acttgtaacg gttgtccacc agtaatcatg atttcctttt ataagtattt
    52801 tctgacctgg aagtttgctt attatatcta aatctttttt agcttctgat aaattaattc
    52861 cccaagatgt gtctcctaat actaagacca tatcttcttc cttaacaact tctttccaat
    52921 tatctataat cttcttttca tgaccatccc aattacttcc aaagatattc attggtttat
    52981 tgactgatgt tgaaaaatgt aaatcaccta tagcatacaa actcataata tcattccttt
    53041 cttgattaca ataaataggc tgtctctttt tagaaattat tttccattaa gaggcagccc
    53101 cattaatatt tagcaacttt tctacaagta gtttttactg aactacttta tcatctacct
    53161 tagtatcctt atattgctct agtaatatat ctatttcttg ttttaattcc aaaatttcag
    53221 ttatattttt atctgtttca gttctatcaa gttttctttg atactgcata gctttatgtg
    53281 tatcacccaa gtcatataac tcttgtattc tatttagagt atctctaata gctatagact
    53341 tcatttggta catagcctga gctttcatta tatcttctct tatttcactc atttcttggt
    53401 ctaaaagaaa ggcagcatca gatgctcttc taagtctttc agcaagagtc tcaggtatgt
    53461 aatggtcata tttatatttt aatgaatgtt ctattgtagc ccagaaattc attgcaagag
    53521 ttcttatttg tatttcacat ataatttcct ttgaacctgc aatggagttt atagggtatt
    53581 ttattataac atgatagctt ctataaccac tatctttgta atttgtaata taatcttttt
    53641 ctcctattat agtcatatca tttcttactt ttatcaaatc aactatagca tatatatcct
    53701 caacaaactg gcacattatt cttataccag caatatcatc aatttcagtt tctatatctt
    53761 ttgcatttat cctttttaac ttagatatta tggaagatat tttttttgtt ctgcctgtga
    53821 caaactctat gggagaatat tcaccttttg ctaaatattc ttttcttata tttttaaatt
    53881 ttattttcaa ttcctctact gcatgttcat aaggtgctag tatctcattc catttttcgt
    53941 attccatatt gctcaccctt tatttgtttt ttatgacctt ctttatttta tcataattta
    54001 ttttgttttt gtatagtaaa gcaattctcc attttccaat atatacccta tttcttctaa
    54061 atcaactaag taacttatga cagatactaa cgaagattta aaaaaatgat actctttata
    54121 attattgctt aaattattgt tatttataat gttttttaat aaaatttcta aagatttcgg
    54181 ttctacaagc aactctctta tttgatgtaa atattttttt aaagcattca aatgtttttc
    54241 gattaattta taagaatctt cttttgatat aacttgcttg ccatgaccta aaactaagta
    54301 ttcaaaatct atatttttta atttttctaa tgaatttatt tgttcttgta tatcatatag
    54361 aaacaagaag tcgtattttg aaagaatttc atcccctatc aataaatcgc caacaaaaat
    54421 cacattatcc ttcgtcaaaa cagctatact accaggtgta tgcccctgaa actcaattat
    54481 atcaaatatt tcattattta attcaatttt tccagctttt atagatttat ctatctttat
    54541 atcatccaac attttatttt ttaagatatt atccataaat acattgctct ttccaccaac
    54601 aatatacttt gaaaataatt ctggattttc tatatataac ttatcgtatt cagatgacat
    54661 tatacgcaca tctttatagt actctttaaa ttgattacaa gctccataat ggtcatcatg
    54721 accatgagta tttataatat atctaacttg tatattattt tcatctaaca ctttatttac
    54781 tctcctaggt cttgaaccac ataaccctgg gtctatcata agtgctgtgt tatcttcaaa
    54841 tatatatatg cctgtatttg tgccaccttt tataaaaaag gtatttcctt ttacttttgt
    54901 aagattcacc ttcttcacct cattttgaca atatttatga tacaatttat tacttgctaa
    54961 tataaaacta tacaagaata atccttttta ttctatctat agttcttttt cttatttcgg
    55021 gttctttaga tatccttatt ttcacattat atgctaaatt tctataaagt aatgactcta
    55081 taaaatattc aactatacct ctatccctaa agtctatatc tatacatcca cttgaataaa
    55141 tatttgagtc taatgtcata agctcatcaa aaaagcttaa tctcttagat ttacttaaat
    55201 aatctttatt tggaataaat ctatcacaat aatctatcct gtaatttact acttcatcat
    55261 ttatttcaaa tccatattgc tttttcatta actctctata gttattattt ttctcaataa
    55321 cattatctaa atttttatta taatcctctt ttttccaatg tccaaacatc ctacaattta
    55381 atggtctaac ttcatatatt aaacatctat tattattatc tttaaatgga catggattct
    55441 tttttgtata ctcctcaaaa taataatcta atattttact tatacagctt ttttttaaat
    55501 caactctatc ttcaagataa caaaatatat tcaaaaactc aattaaattt atacctactg
    55561 actccataca acaatttcca catcccaaac aatttcccac tggcaaggat ttatatacat
    55621 catttagttt ctcaaagagc ttatttttaa tggaataatc tatacaattt aaaatatctt
    55681 tattttttat actagtcatt attattactc cttgttataa ttattaagtt aatacaaaca
    55741 attattataa catatttttt aaatttaatc gcatatggag gtattttatg aatagatata
    55801 caaaaataat taacatgatg ggaagttatt acacaaaaga ctttgaaaaa gaaaaaaaga
    55861 acgtaataaa agttagagaa gttaaagaag atacagttag aaaatttttc ttacaaggtg
    55921 actgtgaggt tcttgtagta tttgaggata ctggaaaaga aatacttatt gatgactttt
    55981 ctcctgaaga agatatcaaa aaatatttag gagcaaaatt cataaataaa aaaagataat
    56041 tatcaaaaat aattctaaaa ttaaaagtta ataaatataa agttataatt ctatataatt
    56101 atagttaaaa gttatctttt attgcttttt tagactttaa gatagctttt tttctatttt
    56161 ttgtaaaata gttccataat tcacattaat aattcacaaa atgatataat ataacaaata
    56221 gataaatttt taaatttcta aatacataat taatattttg cgaggtgatt ttatgcaaat
    56281 ttattttaga gttaaggcta ttggcaaacg taaacctatg ctaaaacttg ccccttttga
    56341 tgtatctgac aacatcaact gcctaaaaga agctatctct gaaattgtat ctaaaaatgt
    56401 tcttgaatat aatgagaaaa tcccagaaaa atcaatagta aacttcttaa ctaatgaaga
    56461 aattgaatct caagctaata ttggaagagt tggatttggc tctatttaca atgagaataa
    56521 acaatctgta gaaaaggcaa tagaagtagc gttacaagca tttgaagatg gaatctacaa
    56581 agtgcttata aatgaagatg ttattgaaaa acttgattct cctataaatc tgaaaagtgg
    56641 agatatattt acttttataa gacttacctt gcttgctgga agaatgtttt aaaataaata
    56701 acttggaggt acataaatgt tagaacgtac tagagtagaa aaaaatccta aaataattga
    56761 aaatttaaaa aacaatttta ctaaatcttc ttcaaaacag tattacgact tagcaaaatc
    56821 tctttgggac tgcatagaaa atagttcata ctattataga gaacctgaag acaaattctt
    56881 aaatatgata aagaaatact acaattgcca ctactacgaa attaaaaata tggacgagct
    56941 cctttctgga cctttaaaaa atgcattaga attaatattt acttctgaac aagtagagga
    57001 actttgccaa ttaattaaaa gaaagatgga ttatacatat tcaggaggat attatcgtaa
    57061 aggatatcgc tcaaataaac ttgtggatta ttttagaggc attataaggt ttatttccag
    57121 ttatgttgat ttgatttatt atgatttttc agttatagat tatcttactc ttccaaataa
    57181 agtcttgtac tatgactcta taatgagtga taaaatagca ctagaaatag acaaaggaaa
    57241 tcaagaagta ataagtgcct tagaagaagt aatatttgga gaaaatcaaa ctgctctact
    57301 tacaagacaa atgataattg gtatcatgaa gagtcatagt gataaatgct atgaaatgat
    57361 ttctaaactt cttttggcag ctaaacaaca agagggactt agacagcaaa ttgtagaaag
    57421 tttagatgag ggtacaattt ctgcatcaat atacatgtta aaaactattt tggacaataa
    57481 tttagagcga tttagttctg ttataagagc cttggataca tggacaggac ttggtttttc
    57541 agaccaaaaa cctggaataa tacgtaaatg tttagaactt tcatataaat cattgactga
    57601 tgaaaagttc cgtatagaat caattaatag caatgataat cttgaagttt attttgcctt
    57661 atgggctact gcaacttaca acatatatga ctcttactct cttattgaaa acttactttc
    57721 ttgtcaggaa aaatataaaa aacttgtagc tctttacttt ttatcgcata cagaaagtac
    57781 atattttaaa cattctatag catctaaaaa cattaagggt gatgatttag agatactagc
    57841 atgggtaatt acaaatttct atgtagaagg ttctgttggc tactactata gttggcaaaa
    57901 cagagaaact agttttgacc tgtcaaacac tcctattgac aaagacttta atgctagaaa
    57961 agaacaattt gaaagtttaa agcatacttt agaaaattta aattcaaaga caaaatcatt
    58021 ttcaggaagt gtatttccat ggacttggat tgaaatttct tctcatgaaa tacttaagtg
    58081 tatgatgact ttaacagcat acgatatgaa tgctcaattt atagatgttc ttatggaaaa
    58141 ttgtcatctt atgagttcag atttaagagc ttcacttata gtaaattttg taaatgaacc
    58201 aaagacagat agacaacgtg aatttttatt ggaatgttta agtgataaga gtgtttcaaa
    58261 tagagaaata gtattgactc tacttagaaa acttgaacta acagacatgg agttggataa
    58321 gataaccaat ctattatctt taaagacaag ctctattaga caaacagtaa taaaaatttt
    58381 acttgaacaa gatgattcaa aattggagaa tattgcttct tcactgataa aatcttcaaa
    58441 ttctcaaaaa tgccttggag gtcttgaaat tgtaaactta ttaaaagaca atactgatag
    58501 aaaagaacta tataaaaagc ttattttact gataaaagat attgataaat ctaaaaagaa
    58561 tacatctcaa gttcaaatac taattgataa tttactaggt aaagcaaaaa atgattattg
    58621 caaagaaaat ggttttggaa tatataaccc agaaaataag gttcaaattc cagaacaatt
    58681 tgattttaaa gataattctc ctagtttatc aaaacttgat aaaattattt caaatacaag
    58741 tttatctact aatgaggatt ctgtttctgt agcaacaaaa aaagaagcaa aaaaaattct
    58801 cgatacagat ataaataaaa taataaaagt ttatgaaaaa tttgatgagc ttattactga
    58861 aaatgcagat tatgaatatg aagttgaaag atggtcaggt aatgtagaca aagtactttt
    58921 aggctattct acttataatt tgactcctat taaatataaa gtttctaatg atactctaga
    58981 taattatcca ttggcagaac tttggagaga aactagtaaa aaacttaact tagactacaa
    59041 attaatactt gatatattat tttatagtat aagtacatgc ggatataatg aaatagaaaa
    59101 aagttggttt tcatctatat attcaaatgt ccttgactct aaaaaacaaa ataaaatatc
    59161 tacaaaactt gaaaaattaa aattcaatgg acttacaaga aagcttctaa gagttttact
    59221 aaatgagttc gaccataaag aaatatttaa ctattgtttg gaagtatact ataaacttag
    59281 aaacttatta actatagaac aatgtaaaaa gcaaagagtt gagtttacta aaaattatta
    59341 tagacaaaat gaatactgcc ctgctttaaa tgcccctatt ttaaattatt ggcttgataa
    59401 agcacattca tttatttatg atgatatttc ttttactaag tttttctaca ctacattttc
    59461 aaattatcag ttgctagaat ataaatttcc tttttatact agcataaatg tatttgctcg
    59521 tgcacatgaa ttaggattaa tgactaaaga gcaagttaat agagaaatga taagtggata
    59581 caacgcaaca aatataataa gaacgttaac aaatcctaga tgggctggtg acacattaga
    59641 aaaatatcct tggcttaaag ataacttaaa aaccgttaca aaccgaatta tagaaattga
    59701 atcacgtaga ggtgatatgc caacagaagt gtctcatcta gctaataata tacaatactt
    59761 tgaaggtata aattattttg taaatatctt agttgctctt ggtaaagaaa ctttccagag
    59821 aggatattca tatggtagca attattcaaa aaaagatgtt ttaagttctc tacttaaaaa
    59881 ttgctatcct acaaaagaag atgattcaaa aaaattaaaa agtttactta aaaataccga
    59941 cattactgag ttaagattga tagaatctgc tatgtatgca cctcaatggg taaacataat
    60001 tgaaaaatat ttaaactgga agggtctgaa aagtacaatt tggtacttcc acgcacatat
    60061 aaatgaagga ttctctgcac aaaaagaatc agaggttgct ctattctctc ctatatcacc
    60121 acagagtttc atggatggag catttgatgt agattggttt ttagattcat ataaaactat
    60181 tggggaagaa agattcaata tactttacaa atcagctaaa tatataacta gtggcggaaa
    60241 ttctcataga cgagcacaac tctatgctga tgctacactt ggaaaacttg ataaagatat
    60301 gctgatgaaa gaaattgaaa caaatagaaa tcaagataaa ttgagaagtt tctctttact
    60361 tcctatgaaa aaaggagacc ttaaagaagc aactttccgt tatgaattta ttcaaaagtt
    60421 tgccaaagaa agcaaacaat ttggtgctca acgtcgtgaa agcgaatcaa aagcttgtgc
    60481 aatagcaatg gaaaacttag ctagaagtgc tggttttgaa gatattaatc gtatgatgtg
    60541 ggctcttgaa acagaaaaat taaatgaagt tgcttatata ttcaaaccaa aaaatattga
    60601 tagaacttca ctttggatag atgttgatga aaaaggaaaa acatctataa aggttcaaaa
    60661 aggtgataag gctttaaaaa gtgtaccaaa agctattgct aaacatccat atgtacttga
    60721 acttaaatct gttcaaaaag atttaaagag tcagtattct cgtgcaaaac agagtttaga
    60781 gcaagctatg aaaaatgaaa tagtatttac agttaaagaa attgccaata tctataaaaa
    60841 tccagttatc tgccctttga ttaaaaatct agtatgggta tgtgatgata atattggata
    60901 tgtttataat gataagtcta agttctttat ttgcaatata gaaggtgaaa tttataagtt
    60961 gacagcgaaa aataaattaa aacttgcgca tccagttcat ttgtttgagt ctggtaagtg
    61021 gagtgagttc caacatgatt tatttagtaa gcaaattgtt caaccattta agcaagtatt
    61081 ccgtgaatat tatagaataa acagtgatga attatctgaa aaaactatat ctcgtagata
    61141 cgctggtcac caagtccagc caaagaaaac tgtagcactt cttagaaatc agctttggac
    61201 tgtcgattat gaagagggat tacagaaagt attttataaa gaaaatatag tggcaaagat
    61261 gtttgcactt gcagattggt tttcaccagc agatatagag cctcctacta ttgaaacagt
    61321 agaatttttt gatagaaaaa cttataaaag cttggaattg gcttctatac ctccaattat
    61381 attctcagaa gttatgcgtg atatagacct tgttgtaagt gtcgctcatg ctggtggtgt
    61441 tgaccctgaa actagccatt ctactgtaga gatgcgtata gcaattgcta aagaactttt
    61501 attattatta aaagttaaca atgtcaattt tgaaggctct catgctctta ttgaaggtac
    61561 tttaggtgag tattctgtgc atatgggttc tggtattgta cataaaaaag gaataggtgc
    61621 tttgaatata cttcctgtac attctcaaag tagaggtaag atattccttc cattcgctga
    61681 tgaagatcct aaaactgctg agattatgtc taagatacta ttacttgctg aagatagtaa
    61741 gattaaagac cctagtatct taagtaattt ataagtataa aaactatccc taaaatatat
    61801 tggactttct taaaaaaatt agaaagtcca atatatacta tactgtaatt tatataaatt
    61861 aaatcataat tacaaataat ttttatatac acttctctta tacattatct ttatggtact
    61921 tattatgtca cacaaaaagt atttttcttc tattttcact agaatctttt aaccctataa
    61981 aatctatata aaaagtcttt ttctagagat ttgtttaata tggcattttc ttgaaaattt
    62041 ataaacctta tctctaatga agatttattt ataggtataa ccttatgtat aaatcttaga
    62101 tttacaaggt atataaaaag ttataaagct agatctttag cgattgctgt gagtataatt
    62161 cttgctacat cactcgttgt tggtattggt actctatcaa gaagtgctca gcaagcagat
    62221 gtagatagat tgaaaagaga gcttggaact gaccatgtat actttaaaaa tataaagaaa
    62281 aatcaattaa agtccataaa atctagcaaa gatattaaaa atctgggtat aacatcttac
    62341 tatacgtcta cagatgttaa tgaaaaatta cctataaaca tactatatgc aaataaaaat
    62401 tatttgacta gtcattctaa acttataaaa ggtcgatttc caaaagctag caatgaggtg
    62461 gtagtcgaga aatggatttt aaatagtatg ggcttaaaac cagagataaa tcaaaacctt
    62521 acttttaaat tatatcaaaa agaaaatcca gaaactttta aagttgttgg aatccttgaa
    62581 gatagattta tagagaaaca aaagggacaa tgtgaaattt tttctaatct caatgaatca
    62641 aaactgaatg attttacaac ttatattgaa tttaatgaag gcagtgatat aaatacaaac
    62701 atagacaata taataaaaaa tgctatgtta gatgagaaaa gtataggaag aaactcaatg
    62761 cttatagaat ctactatgaa aaacggtact ttagataatg caagtaaata cgttgcaatt
    62821 gctatgagtc tatttgctgg tattgtaata tatagtatct atgttatatc aatataccaa
    62881 agagtacagg aatatggtgt cttaagagct attggttcca ctaactttag gattttcaaa
    62941 cttatgcttt atgaactttt catacttgct ctaatagcaa tgcctatagg tatatgtata
    63001 ggtatggggg gtgcacaaat atttaatagg tctgtaggaa atattcaatt tgaaggaaat
    63061 ataaatgtta caccttttgt tattccagat aaaataatcc tcttatcaat tgcatgtaca
    63121 atactaataa tactaattat aagtttcttt acatacttaa aaataagaag aatttctcct
    63181 attgatgcta tcagaaaaac ttttggtgct gataaaaatg taaccacagt taattattta
    63241 atatcaaaat taactttaaa tatgtcagta acaaaatcta tatctgcgaa aaacatcttt
    63301 agaaataaaa aggggttcat tataattgta ttatctatga gtataggagg tataatcgtc
    63361 ataaaagaca attataaata ttctttttta gatgtgcaaa acaaatccac tcaagagcaa
    63421 acatatatga atgcagattt tattctatca aatgtttatc tcaatgaaat tggtagcaat
    63481 acttctttta aagatataaa agggtttagt gatagtcaga tagataaaat aaaaaatata
    63541 gatggaattg acaaagtcaa aactgctagt atcttaaaca caagaataga ggtaaataaa
    63601 ataaatagat tagattacta cgaagctata aattcaaatc cttattataa actaaatcct
    63661 ctgttcataa aaaataagac tactggcaaa tatacactaa agcaaaaact aaaaggttat
    63721 aatgatgaga tgataaagtc attagaaaaa tacttagtaa gtggaaatat aaacttgaaa
    63781 gaaatgaaaa aagaaaattt agctattgta tatgtaccag aaatttctaa aacacagaaa
    63841 tacacaaaga cttttactcc tggtgaagga acaccagcta tagatataaa agtaggtgat
    63901 actataaaag tcaaatatcc aaagggtgga atggatacag gactttatga aagtctaaaa
    63961 gataattatg aatacttaga atatgaattt agagttggag ctattataag ttatactttt
    64021 gcagataatg gtatgtactc tggagatgac ggtgtagatg taataacgag tagcgaatat
    64081 ctaaaaaaat taactggtgt agataagtat aacttagttt atgcagacct taatgaaaat
    64141 gcaaacaaga aaaaaataaa taacctttta ggtaaaattg gaagtgaagt ttctggaact
    64201 acaacagtag atatgatggt agaaaaagaa aattctgata agatgacatc tagaactatg
    64261 atttatagtt atggcatagt agcaataatg ttcataatca gtgtacttaa cataataaac
    64321 aacataagtt ataacttaac ttcaagaact agtgaatttg gaatgttaag agctattggt
    64381 atctctgaga gaggatttaa aaatatgata ctttatgaag gaatattata tggtgtactc
    64441 tcaagtataa ttactattgt gtcaggactt ataatacaat ttaaaatgta ttacacgcag
    64501 ggctttgtat catatggatt aggattttca atagattaca aaatatacat actagttgtt
    64561 gtagcaaata taattgtagg tattctagct acatatattc catcaaggaa gataaataaa
    64621 attagtatag ttgaagctat aaacattaca gaataggagg gataattatg cctatattgg
    64681 aaactattaa tttaggtaag atttatggaa aaaaggaaac atctgtacat gcactaaaga
    64741 atgcaaattt aaaaataaat aaaggcgaat ttgttgctat aattggacca agtggaagtg
    64801 ggaaaagtac atttctacat ttagtaggtg gtcttgagag accaagtaat ggtactataa
    64861 aagttgctgg taaagatata tgttgtcttt cagataaaga actagcaaga tatagaagac
    64921 aaaaagtagg atttgtcttt caacaatata acctaattcc tgtattaaat gtaaaggaaa
    64981 atatagaatt accattaaag ttagacaata aaaaaataga taaagaatat attgaagacc
    65041 ttataaattt attggggctt aatgaaagga aaaatcatct tcccaaccaa ttatctggtg
    65101 gtcaacagca aagagttgct attgcacgtg ctttatctgc aaaaccaagt ataatcctag
    65161 cagatgaacc aacaggaaat ttggacagta aaactacaga agaagtcatg gatttactaa
    65221 aaagttctat aaaaaaatat aaccaaacac ttataataat aacacataat gaaaatattg
    65281 ctagaaaagt agataaaatt atatctataa tagatggaga gcttaaatta acactctaaa
    65341 actatttata gaaaagagga tttaaatgtt attgatttta ggtaatttaa atttaaactt
    65401 ggattttgac tacagaataa taagagaaga aaatgatgat gtagatatat tcatagatat
    65461 taattatcgt agcttagata tagatactga tggttctaac ttatttaatt ctagaataca
    65521 atttccattt gtaagagcat taattctaag gctaaataag aacaatcaat gtatgacaat
    65581 tcatttgtta agagatatag atttattctc tgcttttgcc aactttgaag tggattatac
    65641 tgactctata ataaatataa aaaatcaaaa tgagaaagta attttaaata aatctatcaa
    65701 aaaatagtaa gggggcctta ataggcccca atttttttct tttctaaatt tattatatca
    65761 tgataaataa attagtcaat agaattttct gaatattttt ataatttttt cataaagttt
    65821 tttatatatt atgctttaaa attatatgta atagaaaatt tttccgacta attttaaagt
    65881 tactttatta aatacacata ttcaaaattg acattaaatt attgactttt tatcatttca
    65941 atttagaatt taagttatta tttataacta tttatacgta tctctaataa aactctattt
    66001 atattcaagt ccaataattt atatacaaaa ccaatagaac ctatgttgta caatgaatat
    66061 tatttaccat aataaacttt tagatatatc tcttttaatt cactcatcaa tgggtatcta
    66121 ggattagcac ctgtacattg gtcatcaaaa gcttgttcaa ccatagcatc cagtccttct
    66181 aagaatttag attcttctac tccagcctct tttatagtct taggaagacc tacttttgcc
    66241 tttagttcat ctattgcttt tataaggtta tctacttttt cttggtctgt atttccctta
    66301 attcctgcaa aagaagcaaa ttcagcatat ctttgtatac aatcaggata tctgtattgt
    66361 gagaatgcac ccatcttatt tggagcttct gcacaattaa acttgattac ttcatttatt
    66421 aataatgcat ttgcaacacc gtgttgaaca tgatggaatg cacctaattt atgtgctaaa
    66481 gagtgacata ttcctaagaa agcatttgca aaagacattc ctgccatagt agatgccatt
    66541 gccatttttt ctcttgcttc tttatcattc tttccatttt tatatgcacg aggtaaatat
    66601 tcaaatatta ttttacctgc ttgtagagct aaaccgtcag ctggttcagt tcttaacata
    66661 gatacatatg cttctagtgc atgtgttaaa gcatcaacac ctgatgacgc tgttagtctt
    66721 ggtggcatct ccatcatcat atcagcatca attatagcca tgtttggcat taattcataa
    66781 tctgctaatg ggtattttac gcctgaatct tggtcagtta taactgcaaa tggagttacc
    66841 tctgaaccag ttcctgctga agttgggata gcagcaaagt atgctttttc acccatctta
    66901 gggaatacat atactctttt tcttatatcc ataaatctca tagctaagtc ttggaaatct
    66961 acttctggat gctcatacat tacccacatt atcttaccag catccattgc gctacctcca
    67021 cctacagcta ttattaaatc tggttggaaa tcagccatag ctttagctcc ttctcttgca
    67081 caagctaatg ttgggtctgg agctacatca aagaatgtag tgtgcttaat tcccatctca
    67141 tctaataaat cagttaactt cttagtatat ccattattat ataggaaaga gtctgttact
    67201 ataaatgctt tctttttatc cataacatcc ttaaattctc ttgctgctac acctaaacat
    67261 ccttttttga aataaacttt ttctggtgct ctgaaccaaa gcatattctc tctcctttca
    67321 gctactgtct tgatattaag caaatgtttc actcctacgt tttcagatac tgagtttcca
    67381 ccccaagaac cacaaccaag agtaagagat ggtgccaatt taaagttata taaatcacct
    67441 ataccacctt gtgatgatgg agtattaact aatactctac aagtcttcat agcatttaca
    67501 aacttttcta ctctctctgg atgatttata gagtctgcat ataatgaaga tgtatgacca
    67561 tatcctccat ctgcaactaa tttttctgct ttggcaactg catcttcaaa acttttagac
    67621 ttgtacattg caagtacagg agaaagtttt tcatgtgcaa aaggttcatt taattcaaca
    67681 gattctactt caccaattat tattctagct gtttttggaa cttctacatc tgccatttta
    67741 gctattatat gtgctggttg tccaactatt ttagcattta atgaaccatc aattattact
    67801 acttttccta ctttttccgt ttcttcttta tttaagaaat gtgcaccacg atatttaaat
    67861 tcttttttaa cttcttcata tattttttct ggcacaatta ctgcttgctc tgaagcacat
    67921 atcataccat tgtcgaatga ttttgatact aatattgaac ttacagccat ttttatatct
    67981 gcactctcat ctattattgc agggacattt ccaggtccaa ctccaacagc tggcttacct
    68041 gaggaatatg cagatttaac cattccaggt ccaccagtag ctaatataag gtctgcctct
    68101 gccatgacaa cattagttaa ttcaatcgaa ggaacatcta cccaacctat aagacccttt
    68161 ggtgcgcctg ccttaactgc tgcatcatgt acaactctag ctgcttctat agtacagttt
    68221 ttagctcttg gatgaggaga gaaaattata ccatttctag tttttagtgc tattagggat
    68281 ttgaatattg cagttgatgt aggattagtt gtagggataa ctgctgataa aacacctact
    68341 ggttcagcaa ttcttgtaaa accaaatgct tcatcttttt ctattacacc acaagtttta
    68401 gtgtctttat aagcattata gatatactct gaagcaaaat ggttttttat aaccttatct
    68461 tcaacaatac ccataccagt ctcttccact gccatttttg ctaaaggtat tctttgttga
    68521 ttagctgcag ttgaggcagc taagaaaatt ttatctacct gttcttgcgt aaatgttgca
    68581 aattcttttt gtgctaatct aatttcttct aatctcattc ttaatgtttc aacactgttg
    68641 acaatttcag gactacatac ttcaacactt gatttttcta ctacattttc ttttttctcc
    68701 atactacaaa cctccctaaa ataattatac atctacaatt tttataaatc ttaaatacat
    68761 catctgtact tttaacttat acttaaaata attttataga tttcatctct ataccttaga
    68821 ttttatttac tttactttct taggtcaaga agtttttttc tgaatatttt gtcaatttat
    68881 tttttaacaa atatattact ttttttactt ttactaaatt tttttatcta aatgagatat
    68941 atttcatttg aataatttac tttgactaaa taaaatacta aaatgataat ttatatcttt
    69001 catttttttt acttattaaa ttttatatgt tatttgtaca agcagtaaat aaaatttttg
    69061 tcgtattttt ataaaataac acttattttt gacttgtgtg caatttttct ttttatagca
    69121 actgtatggt tttaaaaaat atacaaacta aaattattct aatttctata agttattttt
    69181 aagtcaatat acaataatta tacttttcat atggataaat aatttttata ttcatccc
//
LOCUS       DAMJVM010000009        69027 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.009, whole genome shotgun sequence.
ACCESSION   DAMJVM010000009 DAMJVM010000000
VERSION     DAMJVM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 69027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69027
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.009"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            529..2448
                     /gene="cooS"
                     /locus_tag="PC523_000663"
     CDS             529..2448
                     /gene="cooS"
                     /locus_tag="PC523_000663"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HDF2474221.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMEADAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            2757..3536
                     /locus_tag="PC523_000664"
     CDS             2757..3536
                     /locus_tag="PC523_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2474222.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            3785..5461
                     /locus_tag="PC523_000665"
     CDS             3785..5461
                     /locus_tag="PC523_000665"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDF2474223.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNEENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKALGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFKFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLERLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            5546..6178
                     /locus_tag="PC523_000666"
     CDS             5546..6178
                     /locus_tag="PC523_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HDF2474224.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVDKVKTEVDKLVEDGVKICDEVYTKVEKALGK"
     gene            6270..7142
                     /locus_tag="PC523_000667"
     CDS             6270..7142
                     /locus_tag="PC523_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HDF2474225.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQDEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSSVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLNKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            7177..7851
                     /locus_tag="PC523_000668"
     CDS             7177..7851
                     /locus_tag="PC523_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HDF2474226.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKVLGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNAENDCAWIKIYERLEAIGQLDNLVEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            7887..8768
                     /locus_tag="PC523_000669"
     CDS             7887..8768
                     /locus_tag="PC523_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDF2474227.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRVAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            8812..10197
                     /gene="lpdA"
                     /locus_tag="PC523_000670"
     CDS             8812..10197
                     /gene="lpdA"
                     /locus_tag="PC523_000670"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDF2474228.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVIEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            10253..11023
                     /locus_tag="PC523_000671"
     CDS             10253..11023
                     /locus_tag="PC523_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2474229.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            11043..11987
                     /locus_tag="PC523_000672"
     CDS             11043..11987
                     /locus_tag="PC523_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HDF2474230.1"
                     /translation="MTFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NPPKIGIQISDVYPESWTDSYKELYKDVAKCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYSHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            12018..13385
                     /locus_tag="PC523_000673"
     CDS             12018..13385
                     /locus_tag="PC523_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HDF2474231.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDSKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRIALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            13435..14241
                     /locus_tag="PC523_000674"
     CDS             13435..14241
                     /locus_tag="PC523_000674"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDF2474232.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            14298..16424
                     /gene="cdhC"
                     /locus_tag="PC523_000675"
     CDS             14298..16424
                     /gene="cdhC"
                     /locus_tag="PC523_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HDF2474233.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            16511..16888
                     /gene="gcvH"
                     /locus_tag="PC523_000676"
     CDS             16511..16888
                     /gene="gcvH"
                     /locus_tag="PC523_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDF2474234.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            17000..18928
                     /locus_tag="PC523_000677"
     CDS             17000..18928
                     /locus_tag="PC523_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HDF2474235.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTIFRKSDFKVTITYVQKQKKLTIINIEEGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKETLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            18953..19825
                     /locus_tag="PC523_000678"
     CDS             18953..19825
                     /locus_tag="PC523_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2474236.1"
                     /translation="MNKNLSEKVKVAFDIKLKNFGNKIEFAYPNQTLALSTTNNKCSL
                     KCAHCNGHYLNNMVPIEDYEEKVQSRNITSFLLSGGCSYDGDVPMSTHIQTIKRLKEQ
                     GYRLNAHLGLMDKESIIELCKYLDIVSFDLVFDKETIREVYKMEKSKDDYIEVYNTIR
                     ENTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIILIPTKGTEYENVEPPELE
                     RVADILCEARINLPYTEINLGCMRPRGVYRKELDQLSIMCGVNKIVLPSRSAKNKAIE
                     MNMTITECKECCVL"
     gene            19822..20760
                     /locus_tag="PC523_000679"
     CDS             19822..20760
                     /locus_tag="PC523_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2474237.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVVWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQINKFFSAGVDKIGIAIDAANKELYEKIKGNNYNEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKGNDITISLFAFTPVRGTKMEKIN
                     QPNIESYRRVQLMSYMINKGYSKEYFTFKNGYLDSIKLDNDIVKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            20807..21622
                     /locus_tag="PC523_000680"
     CDS             20807..21622
                     /locus_tag="PC523_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDF2474238.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLAKELYEKYKNEEYNKKR"
     gene            21773..21964
                     /locus_tag="PC523_000681"
     CDS             21773..21964
                     /locus_tag="PC523_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HDF2474239.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            21983..22399
                     /locus_tag="PC523_000682"
     CDS             21983..22399
                     /locus_tag="PC523_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HDF2474240.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            22415..22765
                     /locus_tag="PC523_000683"
     CDS             22415..22765
                     /locus_tag="PC523_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HDF2474241.1"
                     /translation="MIFDREIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            23034..23813
                     /gene="hisJ"
                     /locus_tag="PC523_000684"
     CDS             23034..23813
                     /gene="hisJ"
                     /locus_tag="PC523_000684"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HDF2474242.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDIGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKANL
                     NKYGEELEDSLLSVHIIKIDDDYYCVDYSIEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEDLIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDIV"
     gene            complement(24129..25004)
                     /locus_tag="PC523_000685"
     CDS             complement(24129..25004)
                     /locus_tag="PC523_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HDF2474243.1"
                     /translation="MKKRLIIILSLVLVLSSVLTIFAYSNIKYNNKLIYSNMIDKRTQ
                     NSVEEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPYNL
                     EKLQDNWMKKFPDYMDVNCRITAFRLFKDFINSNNKFTGDSIDLNVDLDTIMNNKDAK
                     FSTKDVEKFINFFSAIPAKDTSDISKIAEQIKNEWKKRKISFKNNKNISIINGFLRYP
                     ETKNVFIGHTGICIKTKNGILFVEKYGPTSPYQVTKFKNKKDVKNYMFNRLKMSESEI
                     ELPDPVIMENDKLMK"
     gene            25556..26068
                     /locus_tag="PC523_000686"
     CDS             25556..26068
                     /locus_tag="PC523_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474244.1"
                     /translation="MKVKKFKSGIVMALTVMVIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKVNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNVILREEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            complement(26373..27470)
                     /locus_tag="PC523_000687"
     CDS             complement(26373..27470)
                     /locus_tag="PC523_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2474245.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLNILEKYIIEHSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQDIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVDEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSRLEIKKVI"
     gene            27763..29289
                     /gene="glpK"
                     /locus_tag="PC523_000688"
     CDS             27763..29289
                     /gene="glpK"
                     /locus_tag="PC523_000688"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDF2474246.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDKELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTNQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            29330..30445
                     /locus_tag="PC523_000689"
     CDS             29330..30445
                     /locus_tag="PC523_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDF2474247.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGVIGGLIA
                     GFNLSMVIINMIPVIILSVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(30726..31490)
                     /locus_tag="PC523_000690"
     CDS             complement(30726..31490)
                     /locus_tag="PC523_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2474248.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            31749..31839
                     /locus_tag="PC523_000691"
     tRNA            31749..31839
                     /locus_tag="PC523_000691"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31783..31785,aa:Ser,seq:gct)
     gene            31927..32715
                     /locus_tag="PC523_000692"
     CDS             31927..32715
                     /locus_tag="PC523_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HDF2474249.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISDNN
                     ELDPFIKKGLELMVDGVDAVTIRSILESQIQITSKRHKEGIAIFDSAGGYAPTMGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEISE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            32736..33476
                     /locus_tag="PC523_000693"
     CDS             32736..33476
                     /locus_tag="PC523_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2474250.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDNLQNAKFKKVGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAVVKDNSREKIIQIGKMLNEMNSYIRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSNDK"
     gene            33654..34022
                     /locus_tag="PC523_000694"
     CDS             33654..34022
                     /locus_tag="PC523_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474251.1"
                     /translation="MGNKNTKYKDKTRERYNKKVENLNKEKLNKANKESKKVDKIDDI
                     INKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVKAL
                     FSIFVIVCFLIAFKYVVNPV"
     gene            34114..35574
                     /locus_tag="PC523_000695"
     CDS             34114..35574
                     /locus_tag="PC523_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDF2474252.1"
                     /translation="MNRCKFAKLIITLMIVLLLTGCSLKKEDNYKLDPENPISIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGSERGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELSEYIDEYVEEGRINSKGEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFNGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLEVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDLNKINELV
                     KSGTSKKDAVAQFNNDKNFETWFESFKAELEKAIKK"
     gene            35627..38656
                     /locus_tag="PC523_000696"
     CDS             35627..38656
                     /locus_tag="PC523_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2474253.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEARINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIRIISNDLIYLLRKNSVTGAFIILSDKNIKDNPVDSNGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQEGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRIMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNDDMAFENVISNGYSLKEKFWNGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLNLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYVASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNADNVFCTKEFFSVFITESKFEEYGYMEK
                     SKFLKKLRGLDKYLQESYETENTYIYKFESKDKKPKWVRLNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKVAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSINYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGI
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            38733..40799
                     /locus_tag="PC523_000697"
     CDS             38733..40799
                     /locus_tag="PC523_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF2474254.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLKNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLISSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKVIKCSQGDGGSVTYISPHDSEEEAL
                     YIAKEIIDEIKKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     misc_binding    41327..41537
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            41761..42576
                     /locus_tag="PC523_000698"
     CDS             41761..42576
                     /locus_tag="PC523_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2474255.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDSGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKNVTEGMAAAIKKADNNKEFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            42604..43281
                     /locus_tag="PC523_000699"
     CDS             42604..43281
                     /locus_tag="PC523_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2474256.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            43340..44062
                     /locus_tag="PC523_000700"
     CDS             43340..44062
                     /locus_tag="PC523_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF2474257.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEVLLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
     gene            44709..45860
                     /locus_tag="PC523_000701"
     CDS             44709..45860
                     /locus_tag="PC523_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDF2474258.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     GIKEVRQDVAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKSAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVNNLKNTLKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSHV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            45906..47081
                     /gene="thiI"
                     /locus_tag="PC523_000702"
     CDS             45906..47081
                     /gene="thiI"
                     /locus_tag="PC523_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDF2474259.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGKFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIDA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDKINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            47217..48020
                     /locus_tag="PC523_000703"
     CDS             47217..48020
                     /locus_tag="PC523_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2474260.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEEPSKDKNKVIKDVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGGESIAKVYVTCTEHPDGVEMARDVHQSMKDLIASFAQDPSIIPGASKSNDDFRKY
                     LLDNKYKKGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKPDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            48263..48904
                     /locus_tag="PC523_000704"
     CDS             48263..48904
                     /locus_tag="PC523_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDF2474261.1"
                     /translation="MINNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKAISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDVGILTGNITTAAQALGLGSCIIGMIRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYREILDIPEGYSVPFGIAIGIPDGNLPNAREVVYKVSRV"
     gene            complement(49303..50037)
                     /gene="pflA"
                     /locus_tag="PC523_000705"
     CDS             complement(49303..50037)
                     /gene="pflA"
                     /locus_tag="PC523_000705"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDF2474262.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSDGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDVDNLEKLGKFVATLKNVERFELLPYHSMGIHKWENLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(50197..52428)
                     /gene="pflB"
                     /locus_tag="PC523_000706"
     CDS             complement(50197..52428)
                     /gene="pflB"
                     /locus_tag="PC523_000706"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDF2474263.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDKAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            52721..53782
                     /locus_tag="PC523_000707"
     CDS             52721..53782
                     /locus_tag="PC523_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDF2474264.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDILFITVVLFLVGFLLITKGADIF
                     INCTVEIGRKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFLSPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKSRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVRIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAISAIPNNIPILSQNRQLDIPFMILLILIVIIPTLKSNRLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            53873..55486
                     /locus_tag="PC523_000708"
     CDS             53873..55486
                     /locus_tag="PC523_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2474265.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLENFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            55893..57878
                     /locus_tag="PC523_000709"
     CDS             55893..57878
                     /locus_tag="PC523_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2474266.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSSVLGVSTRTIRNYIN
                     QINRELKDGIAKIVKNEKGTYSIRVEDESKLIEIVNFNRVKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLVLDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIISALRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIEDAILLKNSINIKSIK
                     KIEKNIPFIGKLIEEDKFFILNKGTFMENLEEMLEKLVLKGIIDIEFKENVLKREKKS
                     STALDNYIALPHSVSPNEENLYLAFGILENPVIWDTKEIRIIILMIIPDKNVGSTELI
                     IKSYEEVLEIGRDKKIVEKLSKVKSFDEFYKIITTRR"
     gene            57883..58317
                     /locus_tag="PC523_000710"
     CDS             57883..58317
                     /locus_tag="PC523_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDF2474267.1"
                     /translation="MDPVIFIIVSFIVVYLLNIFLGYLQIKDFNKNYIELKRKGRVAI
                     GRKKGRISSGAIVLILINDDGVIIETRKMQGVTVVARVKSFEGLVGKNLKYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDMSNLSTDEECRMEVV"
     gene            58346..58906
                     /gene="srlA"
                     /locus_tag="PC523_000711"
     CDS             58346..58906
                     /gene="srlA"
                     /locus_tag="PC523_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDF2474268.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            58970..59338
                     /locus_tag="PC523_000712"
     CDS             58970..59338
                     /locus_tag="PC523_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HDF2474269.1"
                     /translation="MSFNKVKIIKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            59363..59929
                     /locus_tag="PC523_000713"
     CDS             59363..59929
                     /locus_tag="PC523_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDF2474270.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            59970..60335
                     /locus_tag="PC523_000714"
     CDS             59970..60335
                     /locus_tag="PC523_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDF2474271.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTNDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            60435..61208
                     /gene="srlD"
                     /locus_tag="PC523_000715"
     CDS             60435..61208
                     /gene="srlD"
                     /locus_tag="PC523_000715"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDF2474272.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAK
                     EIEMEFGCKSVGYGCNAIDEIQVMSTIKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFMDVANILTFYASKEAEYCTGQSINITGGQVM"
     gene            61556..63664
                     /locus_tag="PC523_000716"
     CDS             61556..63664
                     /locus_tag="PC523_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2474273.1"
                     /translation="MDITKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDEVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGSPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYIQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKRYLYSLELSFLIDECGVKENDIREL
                     VALYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSKLYGQDREFYVSNISKFIEESLRNTSNKINNVSDEPDTKSNKTDYISNDLY
                     SLSGKSIKDNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKVCFDMFMTDTATLCD
                     LFIPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFFMELAKKMRLNDYPF
                     VEKSTYLEKVIEPLKCVDKNLDIEKLKNNYFTIHNPVAWEDKKFETPSGKYELYSESI
                     KNLGINPTPVYINNKYKEIEDKNENISFRLLTNHHADTLFSQHFMDKKSIAQAYINQR
                     MAKKVGIKDKDTVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKKHGNPNILTDTGI
                     SDFGGQVTYNESIVEIIRQN"
     gene            63751..64086
                     /gene="spoIIAA"
                     /locus_tag="PC523_000717"
     CDS             63751..64086
                     /gene="spoIIAA"
                     /locus_tag="PC523_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HDF2474274.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            64100..64528
                     /locus_tag="PC523_000718"
     CDS             64100..64528
                     /locus_tag="PC523_000718"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HDF2474275.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            64551..65315
                     /gene="sigF"
                     /locus_tag="PC523_000719"
     CDS             64551..65315
                     /gene="sigF"
                     /locus_tag="PC523_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HDF2474276.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            65602..66039
                     /gene="spoVAC"
                     /locus_tag="PC523_000720"
     CDS             65602..66039
                     /gene="spoVAC"
                     /locus_tag="PC523_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HDF2474277.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            66193..67206
                     /gene="spoVAD"
                     /locus_tag="PC523_000721"
     CDS             66193..67206
                     /gene="spoVAD"
                     /locus_tag="PC523_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HDF2474278.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLTDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGEGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGIDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            67219..67581
                     /gene="spoVAE"
                     /locus_tag="PC523_000722"
     CDS             67219..67581
                     /gene="spoVAE"
                     /locus_tag="PC523_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HDF2474279.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            67765..68781
                     /locus_tag="PC523_000723"
     CDS             67765..68781
                     /locus_tag="PC523_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2474280.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAMFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
BASE COUNT        26882 a         7627 c        12878 g        21640 t
ORIGIN      
        1 ttatataaaa aataattata gcttcaaatg aatatataca aatgttcaat ttgaagctat
       61 aattaatatt aaagtaggaa aaataaaaaa attcaaaaaa attagtccaa aaaatgctat
      121 tctaagaagt ttttactaat taagtttagg gagcaaaaat ctgtaataag tccaaatagt
      181 agtgaaagat aataaataca caaacaatct gtaataatat aaaaatgact agaaaaattt
      241 caaatttgat ttaaaaaaat caaaacataa tttaattaaa ctgaagtatc aaaaaagtag
      301 gtttagatat taacaaatag ccaaaaataa gcgataaaat caacttaaat taagtacaaa
      361 aaataaaatt tttatgtatt atctaatttt gttaaaaaaa tatttaaatc taaagcaaag
      421 cactgaaaat tagtatataa tcaacttaga aagcatttat tttattattt tatgacatga
      481 agtcagtcac ctgggggatt ttaaaaatca aaaaaagggg ataggaatat ggatgaaaaa
      541 atgttaacaa ttgatttaaa cagccagaag atgatagcta aagctagaga agaaggcgta
      601 gaaacaatgt atgatagaaa agaaggcttt aaggcacaat gtggttttgg tcttcaaggt
      661 gtttgctgta gaatatgtgg aatggggcca tgtagaataa gccctaagac tccaaggggg
      721 ttatgtggag cagatgagca tactatagta ggtagaaact ttgcaagaat ggttgctggt
      781 gggacagctg cccattcaga ccatgctaga gatatagcac atacattagc attagctgat
      841 ccaaatggaa actataaaat aagagacgaa gctaagttaa ttactttagc taaagaatgg
      901 gatgtagaga cagaaggtag agatatctat gatgtagcac atgaagtggc agaaattgca
      961 ttaatggaat ttggaaaacc ttttgggaca ttaagattta taaaaaatgc accagaacca
     1021 agacaaaaaa tatggaaaga atatgctata gagccaagag ctatagatag agaaattgca
     1081 acaataatgc actctactca tataggatgt actggtgata tagatagttt aatacacatg
     1141 agtcttagaa cttctatggc tgatggttgg ggtggttcta tgataggtac tcgtctaagc
     1201 gatatcttat ttggaactcc agtgccaaga agaacagagg ctaacttagc agtattagaa
     1261 gaaaataaag ttaatataat attacatgga catgagccag cattatcaga aatgatagtt
     1321 ttagcatcag aagaaccaga cctagtagct ttagctaaag aagttggagc tgatggaata
     1381 aacttagcag gtatgtgttg tacaggtaat gagattacaa tgagacatgg tgtaaaaata
     1441 gcaggagact tccatcaaca agaacttgca ataataactg gagcagttga agctgtaata
     1501 gttgatgttc aatgtatatt ccctgcatta gctagagttg cagattgtta ccatactaaa
     1561 tttgtaacaa cttctccaaa agctaagata acaggttcaa cttatattga atttagagaa
     1621 gaacaagcac ttgatgatgc taaagcaata gttaaagaag ctattttaaa ctttaaaaat
     1681 agagataaat caaaagtact tatacctgaa ttaaaatcag gagcaacagt tgggtatagt
     1741 gtagaagcag tagtaaatca attagacaga gttgtaaact ctcatataga cccagcagga
     1801 acagttaagc cattaacaga ctgtctaaaa gctggagtat taagaggagc agctggtgta
     1861 gttggatgta acaatgctaa aggagtttca aatgaagctc acgtaacaat aatgaaagaa
     1921 ttaatcaaaa acgacataat agtagttact acaggatgtg gagcttcagc agcagctaag
     1981 tttggtttaa tggaagctga tgcggctgaa aaatatgcag gtaaaggtct tgcaactgtc
     2041 tgtaaattag taggaatccc accagtacta catatgggtt cttgtgttga tataagccgt
     2101 atactagatt tagttggagc agcagctaat tacttagata tggatatgtg tgaccttcca
     2161 gttgtaggta tagctccaga gtggatgtca gaaaaagcag ttgctatagg atgctatgtt
     2221 gtagcttctg gtatagacac ttatctagga ataatgcctc caatagctgg ttcttcaaga
     2281 gcagtagata tattaactag cgaattaaaa gataaagttg gagcgacatt tactgtaaat
     2341 acaaatccaa aagagttagc agctacaata atagaagata tagagaagaa acgtgttcac
     2401 tttgaagcat tagttgaaga aaaaatggcg gaaaaagctg aagcttaaga aatagcgaag
     2461 ttatagaata aacgttttta taagaagttt atggctgaat aaagttgaat aacgtaaaat
     2521 attaaagtaa agaacttaaa atataatgaa tatactgaat aaataaaata taaacaatat
     2581 taaagaataa taaaactata catgctgctt tgaaaagcta gtagggtaaa tctaattggt
     2641 tcctgtaagg actagctgta gtttattttt agtgtataaa aatagagagt ataaaaaata
     2701 tttaactata aaattaacta ctaaaaagca aatagtaata aaaggtggta tggattatga
     2761 agatagcaat aacaggtaaa ggtggagtag gtaaaacgac tttttcttca atgctctcta
     2821 ggatgtttgc tgaagacgga tacagagttg ttgctgttga tgccgaccct gatgctaatt
     2881 tagctttagc actaggtttt ccaaaagaag tatatgaatc aatagtacct atatcagaaa
     2941 tgaaaaaatt agtttcagat agaacagctg cttctgtagg ttcatttgga aaaatgttta
     3001 agatgaatcc taaagtagac gatatccctg aaaatttctg taaagaatac aatggtgtaa
     3061 gattacttac attgggaaca gtagattctg gtggtacagg atgtgtatgt ccagagcatg
     3121 ttcttttgaa gaggctctgc tcacatctaa tactacaaaa taaagatgta gtggttatgg
     3181 atatggaagc gggaattgaa cacttaggaa gaggaacagc ccaaggtgta gatgctttta
     3241 tagtagttgt agaaccagga gagagaagtc tacagactta tagaaaagtt aagaaactag
     3301 gacatgatat aggtgtaaat aaagtatttg tagtgggaaa taaaattaga aataaggaag
     3361 atgaagaatt tataatccaa aacttagaag atggagaatc tttaggattt atttattata
     3421 accaagatgt tattgattct gatagagcta atcaatctcc atatgattct agtgaaacaa
     3481 cgaaagaaca aattaaggca ataaaagata aattaatgtc acttaaagat aaataaaata
     3541 aatagaattt ttgatttggg attttaagtt ggttaaaggt tagacatagg caatagatag
     3601 gtaataagta agtaattaga taaataacta gctattaagt ttgaatattt taatagctta
     3661 gtagctagtt tttaagtaat tttacatgta taattatctt ataaaatata atttttacat
     3721 attaatacaa ttttcacaaa aattatattt taaatctatt taggttgtgg gaggagattc
     3781 agttatggga tttaaatctg acattgaaat agctcaagaa gctaagcctc aagatattag
     3841 agaggttgca aaaaaattag gactaggtga agatgatgta gaactttatg gtaaatacaa
     3901 agcaaaagtt gactacaatc tactaaaaag agaaactggt aaaaaagcta agttaatact
     3961 aactacagct ataaacccaa ctccagcagg tgaaggaaaa actactacta ctataggtgt
     4021 tgctgatgca tttgctaaat tagataaaaa tgtattagtt gctttaagag aaccatctct
     4081 aggaccagta tttggtgtta aaggtggagc agcaggtgga ggatatgcac aagttgttcc
     4141 tatggaagat ataaacttac acttcacagg agattttcat gctataggag ctgctaacaa
     4201 tcttttagct gctatgcttg ataaccatat tcaccaagga aatgcactta gaatagaccc
     4261 taagaaaata acttggagaa gatgtgtaga catgaacgat agacaactta gaaatatcgt
     4321 tgatggtatg ggtaaaaaag gtgatggagc agttagacaa gatggattcg atataactgt
     4381 tgcttctgaa ataatggcag cgttctgttt ggcaagtgat atatctgatt taaaagaaag
     4441 attaggaaat ataatagttg gatacagtta cgaaggtgaa cctgtaacag ctagacaatt
     4501 aaaagctaac ggtgctatgg ctgcattatt aaaagatgct ttaaaaccaa acctagttca
     4561 aactctagaa ggaacaccat catttgtaca tggtggacca ttcgcaaata tagctcatgg
     4621 atgtaactct gttatagcta caagaatggc tatgcacttt gcagattatg taataactga
     4681 ggctggtttc ggtgctgacc ttggagcaga aaaattctta gatataaaat gtagaatggc
     4741 taacttaaaa ccagatgctg ttataatagt tgctactgta agagcattaa aatacaatgg
     4801 tggagttgct aaagcagact taaatgaaga aaacctagaa gctcttgaaa aaggacttcc
     4861 aaacttatta aaacatgttg aaaacataac tcaagtatat ggattaccag cagttgttgc
     4921 aattaacaga ttcccacttg acactgaagc tgaacttaaa cttgttgaag acaagtgtaa
     4981 ggcattaggt gtaaatgttg ctctttctga agtatgggca aaaggtggag aaggtggaat
     5041 tgcagtagct aaagaagttt taagattatt ggatgaagaa gaaaataact ttaaattctg
     5101 ctatgaagat gatttatcta taaaagacaa gataaatgct atagctacta agatttatgg
     5161 agcagatggt gtagattata ctccagaagc tgataaggaa atagctaacc tagaaagact
     5221 tggatttact aaagttccag tatgtatggc taagactcaa tattctttaa ctgatgacca
     5281 aactaaatta ggtagaccaa caggatttag aataactgta agacaagcaa caatctcagc
     5341 aggagctgga tttatagttg cacttactgg agaaataatg aagatgccag gattaccaaa
     5401 agttccagca gcagaaaaaa tagatgttga tgaaaatgga gtaatagctg gattattcta
     5461 aggaaattaa tttataaata aagagtagga aatatctcta aaataatttg agatattttc
     5521 ctacatcctt ttaaggagga ttatcatgaa aatagcagat aaaacttgtg ttgattttgt
     5581 agaagtacta tcttctaaag aagcagtacc aggtggcgga ggagctgctg cactagtagg
     5641 tgcaataggt atggctctag gcagtatggt atgtaatctt acaataggga agaagaagta
     5701 tgctgagtat gaagaaagtg taaaagagat actaagtaag gcaggaaagt tagaaaaaga
     5761 tttattaaaa atgatagatg atgatgcaga atgtttctta ccattatcaa aagcttacgg
     5821 gcttcctaaa gaaaccgaag aagaaaaaag aataaaagca gaaacaatgg aaaaagcatt
     5881 aaaagttgct tgtgaagtac cattaaacat agtaagagtt tgctatgaag ctattaaatt
     5941 acatgaagat ttagttgata aaggctcaag acttgctata agtgatgttg gtgtaggagt
     6001 tcagtgttta agagctgcta tacttggagg acaattaaat gtagttataa atataaattc
     6061 tatacaagac aaggaatatg ttgataaagt aaaaacagaa gtagataagt tagttgaaga
     6121 tggtgtaaaa atttgtgatg aagtttatac caaagttgaa aaagctcttg gaaaataatt
     6181 tttaaatgac gaaagtagat actatgttat taatatatgg tatatagaat aaattaattt
     6241 aagtttgctt gtaggggagt tgtgaatgta tggaaggaat gtcaactaaa ggacaaatta
     6301 taaaaggaaa acctgtagct gataaaatta gtgaggaatt aataaaagaa gtcgatttac
     6361 tagttaaaga aggtataaat ccgaaactaa caatagttag agttggggca agaagtgatg
     6421 atttatctta tgaaagagga gcattaaaga gatgtcaaaa cattggtata acaacagaag
     6481 tgttagagtt agctgaggat ataacacaag atgaatatat tgatgtatta aagagagtta
     6541 atgatgacaa aaatgttaat ggtatattat gttttagacc tcttccaaag cacttaaatg
     6601 aagaagttat aaaatatgtt attgcaccag aaaaagatgt agactgtttt agcccaataa
     6661 actctgctaa ggtaatggag ggagataaaa gtggatttcc accatgtact cctacagcag
     6721 tagtggagat attaaaacat tataatgttg atttaaaagg aagtaaggta acagttcttg
     6781 gaaggtcaat ggtagttggg aagccagtat caatgctttt attaagcgaa catgcaactg
     6841 ttacaatatg tcattcaaaa actaaaaatc tatcaagtgt agctgcagag gctgatgtat
     6901 taatagcagc tattggtaga gctaagatgg ttgatgaaag ctttgttaag gatggggcag
     6961 tagttataga cgttggtata aatgttgatg aagaaggaaa tttatgtgga gatgttgata
     7021 caaatgcagt tttaaacaaa gtatcaatga taactccagt tccagcgggg gttggttcag
     7081 ttacaacatc tatacttgca aaacatgtag ttaaggcttg taaattacaa aacaataaat
     7141 agatatatat gtggaaggtt taggggggaa gtagatatga taatttctga aaataaacca
     7201 ttagaagaag tgctagggta cttgaaggat tttgacaagc tggttttagt tggttgtaat
     7261 caatgtgcag caacttgtaa aagtggtgga gaagaagaag ttttaaaaat gaaggaaact
     7321 cttgaaggtg aaggcaaaaa agtcttagga tatgtaatgc ttgaccctgc ttgtaacctt
     7381 ttaaagtcta aaaaggattt aaaggctcta aaagaagaga ctaaggaagc tgatgcagtt
     7441 ttatctttag catgtggaga tggtacacag acaatagtta agaatctaaa agataagcca
     7501 gtttatccag ccaacaatac tttgtttata ggggaagtac aaagagttgg agaatatgaa
     7561 gaagcctgta aggcttgtgg agactgtgaa cttggttgga caggtgggat atgtccagta
     7621 actatgtgtg ctaaaggtct tatgaatggg gcttgtggtg gagctaaaaa tggtaagtgt
     7681 gaagtaaacg cagagaatga ctgtgcttgg ataaagatat atgaaagatt agaagctata
     7741 ggacaattag ataacttagt agaaataaga ccaccaaaag actattctaa gcaaaataat
     7801 ccaagaagtt taagtgcaaa gaaaaagaaa gaagcagcag caaattcata aaagtttttt
     7861 ttagaaggtt aaaaggggga ttagagatga gcttattaag agaaacatta gaaagtggaa
     7921 aatttgcagt tactactgaa atggctcctc caaaagggac tgacctttca catttaatag
     7981 aatgtgcaaa gccgttagtt ggaagagttc atgcagcaaa tgtaacagat tttcaatcgg
     8041 cagttatgag agcaacttct cttgctacat gtaaattatt aaaagatgct ggattagagc
     8101 cagttattca aataacaggg agagatagaa ataggatagc tatacaagga gaaatgttgt
     8161 cagcaggtgt atttgggata aacaatctgt tggcactaac aggagaccat actagcgtag
     8221 gagaccatcc acaagctaag ggagtgtttg acttagattc tgtggggata cttcaaactg
     8281 ctgagacttt aatggcaggt acggatatgg ctggtaataa attaaaaggt tctccagatt
     8341 tttatctagg agcatcagta actccagaat attctccaat agaagttcaa ttattaaaaa
     8401 tgaaaaagaa aataaaagca ggggctaaat ttttccaaac acaagcactt tatgacataa
     8461 atacaatgag aaagtttaga gaattaacaa gggatatgga ttgcaaggta ttagcaggaa
     8521 tagtaccttt aaaatcacca gggatggcta aatttatgac tgcaaatgta ccaggaatat
     8581 ttgttccaga tgaacaaatc gaaagattga gagtagctgg aaaagaaaac tgggtaagtg
     8641 aaggaataaa aatggcagga gaacttatca aacaattaaa agaagaagat ttatgtgatg
     8701 gtgttcatat aatggcaatt ggggcagaag agaatatacc tgctatactg gatgccgcag
     8761 gtttatagta ttgcaagatt gtagattata ttaggtttgg gggaattaag gatgaagata
     8821 gtagtagtag gtggtggacc aggaggatat gtagcagcta taaaagcttc tatgcttggt
     8881 gcagatgtaa cagttattga aaaaagaaga gttggaggaa cttgcttaaa cgcaggatgt
     8941 ataccaacta aagcacttct tgcttcttca ggagtattga atactgtaaa agaagcaaag
     9001 gattttggta tagaaataga tggtacagtt aagccaaatt tcactgccat aatggaaaga
     9061 aaaaacaaag ttgtaaacca attaataagt ggtatagagt tcttatttga aaaaagagga
     9121 gtaaacttag ttaatggttt tggaaagtta attgataaaa acactataga agtaactaaa
     9181 gatgatggaa cagtagaaac tataaaagca gataagataa tactagcaaa tggttctgtt
     9241 ccagttgtac caagaatgtt cccatatgat ggaaaagttg taataactag tgatgaagtt
     9301 cttggacttg aagagatacc tgaatctatg ttaatagtag gtggtggagt tataggatgt
     9361 gagatagggc agttctttag agccttagga acagaagtaa ctatagttga gatggttgac
     9421 caaatactat taaatgaaga taaagatgta gctaaacaac tacttagaca atttaagaaa
     9481 gataaaatta aggttatcac aggtattgga gtacaaactt gtgaagtagt tgatggtaag
     9541 gctgttgcaa ctctttctaa tggaaaggtt atagaagcac aatatgcatt agtatgtgtt
     9601 ggtagaagac ctaaccttga taattctgga gtagaagata taggaattga aatggaaaga
     9661 ggaaaagtag tagttaatga acatcttgaa actaatgtag aaggtatata cgcaataggt
     9721 gatataatag atactccttt cctagcacat gttgcttcta aagaaggtat cgtagcagtt
     9781 gaaaatgctt taggtaagac taaagtagta gattatagag caattccaag atgtgtttat
     9841 actgaaccag aagttgctgg tgttggtaaa actgaaaagc aacttgaagc tgaaggtgta
     9901 gaatacaatg taggtcaatt tgattttaga ggtcttggaa aagcacaggc tataggacat
     9961 ttccaaggat ttgtaaaagt tatagctgat aaagaaactg ataagataat aggtgcagca
    10021 gtagtaggac ctcatgctac agatttattg acagagttgt cacttgctgt tcatctagga
    10081 ttaacagtag aacaagtagg ggatgcaata catccacatc caagtttatc tgaaggacta
    10141 atggaagcgc ttcatgatgt acatggggaa tgtgttcatt ctgtacctaa attataatag
    10201 acagacagag tacataaggc tctgtggcta gatttaaggg ggaagtctta aaatgggata
    10261 caatatagct gttgcaggaa aaggtggaac tggtaaaaca agtctcacag ggcttttgat
    10321 tgattatttg gttaaggaca aaaagggacc agtattagtt gtagatgctg atgccaatgc
    10381 taacataaat gaagtattgg gtatcgaagt tgaggcaaca ataggagaaa taagagaaga
    10441 agtaaatcaa agagaaaagt taggcaatgc attccctggt ggtatgacaa aggcacaata
    10501 tcttcaattt agattaaatt ctataattga agaaggcgaa ggatatgact tattagtaat
    10561 gggtaggtca gaaggtgaag gatgttactg ttttgtaaat ggaatactta gagaacaagt
    10621 aaacaaaata tctggtcatt acaaatactt agtcatggac aacgaagctg gtatggaaca
    10681 tctaagtaga aaagtcacta gacatgttga tacactttta ttggttagtg attgctcaag
    10741 acgtagcata caagctgttg ctagaataag agatttagct gaggaactaa agttaagtgt
    10801 tggaagaata cttctaattg taaacaaagt tccaaatggg gttatgaatg atggtgtcaa
    10861 agaagaaatt gaaaagcata acctagagtt aatcggtgtc gtacctatgg atgaattgat
    10921 atatgaatat gattctacag gtattccttt agttaactta cctgaagatt caaaatctaa
    10981 agtggctatg aaagaaattt ttgctaaatt agaattaaaa taggtttaag ggggaaaaaa
    11041 atatgacatt taaaatgtct actcaaaaat attctggaaa aatatcagaa gttgaagtag
    11101 gaatagggga aaaagcaatt aaattaggtg gggaaaatgt attacctttt tatagttttg
    11161 atggagaagt aggtaatcct ccaaaaatag gtatacaaat atcagacgtt tatcctgaaa
    11221 gctggactga ttcatataag gagttataca aagatgtagc taaatgtcca gtagaatggg
    11281 ctaaatatgt tgaagctaac acacaagcag attttatttg tttaaaattt gatggttctg
    11341 acccaaatgg attagataag tcagttgatg aatgtgctga tgtagctaag gcagtaattg
    11401 aagcaataaa attacctcta gttgtagctg gttcaggaaa tcatgaaaaa gatggtaaat
    11461 tgtttgaaaa attagctcaa acattggatg gacataactg cttatttatg tcagcagtag
    11521 aagataacta taagggcgta ggagcatcag ctggtatggc ttattcacat aaagtagggg
    11581 ctgaatcttc tgttgatata aacctagcta aacaattaaa cgtattgtta actcaattag
    11641 gtgttaaagg tgaaaatata gttatgaatg ttggatgttc agcagttggt tatggatatg
    11701 agtatgttgc atctacaatg gatagaatta gacttgcagc atttggtcaa aatgataaga
    11761 cattacaaat gcctatcata acaccagtag cttttgaagt aggtcatgtt aaagaagcta
    11821 tagctccaat agaagatgag ccagattggg gttgtccaga agaaagaact atagcaatgg
    11881 aagtttcaac tgcagcaagt gttttagtag gtggttcaaa cgcagttata cttcgtcatc
    11941 caaaatcaat agaaactata aaagaattgg ttaacgcatt agcttaatta aattgttaag
    12001 gttaggggga aaaaataatg gcattaaaag ctttagatat atttaaatta acaccaaaga
    12061 aaaactgtaa ggattgtgga ttcccaactt gtatggcttt ctctatgaag gttgcttcag
    12121 gagctgtaga agtaggtaaa tgcccacata tgtctgatga cgctatagca aaattatcag
    12181 aggctactgc acctttaatg aaagctttaa aagttggtgc tggtgcatct gagtatgaac
    12241 taggtggaga aacagtatta tttagacatg aaaaaacatt agtaagtaga aatagatatg
    12301 cagtttcatt ctgtacttgt atgagtgatg aagcagtaga ttctaaaata gctaacatga
    12361 aaaaagttga ctatgtaaga attggtgaac aaatgaaagt tgaaatggct gttttagagt
    12421 attgtggaga taaagatgcg tatttaaaat taatagataa aataaaaggc agtggattag
    12481 aagtagctta tattttagct tgtgatgatg cacaagtagt taaagaagct gttgaagtat
    12541 taaaagatgc tagaccaatg gtgtatggag caactaaaga gaactacaaa gatatgatag
    12601 aagtagttaa aggagcaagt ttaccactag gtgtaaaagc cggtagccta gaagaattat
    12661 atgaaacagt tgagttaatt caagctgctg gatataaaga attagtatta gatgtaacag
    12721 gagaaaacat aaaagatact tacactaatg ctatacaagt tagaagaata gctttaaaag
    12781 agcaagatag aacatttgga tatccttcaa tagtatttgc aaatagatta tctaattcaa
    12841 accctatgat ggaagttgct ttatcatcaa tattcactat aaaatacggt tctataatag
    12901 taatagatga tattagttat gctaaggcat taccattatt tgcactaaga caaaatatat
    12961 acactgaccc acaaagacca atgagagtcg aaccaaagat ttatccaatc aacaacccag
    13021 atgaaaattc tccagtatta gttactgttg actttgcatt gacttacttc atagttgctg
    13081 gggacataga aagatcaaaa gttccagtat ggttagtaat accagatgca ggtggatatt
    13141 cagttcttac atcttgggct gctggtaaat ttggtggaaa ctcaatttct gctttcatca
    13201 aagaaagtaa agtagaagaa gtaactaatt gtaaagacct tatcattcca ggaaaagttg
    13261 cagtacttaa aggtgatata gaagataact taccaggatg gaatgtagtt ataggaccag
    13321 aagaatctat ggaattacct aagttcttaa aaggatacca agaaaaagca tgccaaacaa
    13381 actaataaaa tattaactaa ctattaatat taaatttaaa gggggacatt ataaatggaa
    13441 aaatttatga ttataggtga aagaatacac tgtatatcac cttcaataag aaaagcatta
    13501 gcagaaagag acccagctcc aatcttaaaa agagcaaaag aacaattaga ggcaggagca
    13561 cactatatag attttaatat aggacctgct gaaagagatg gcgaagaaat aatgacatgg
    13621 ggaatcaaat tacttcaatc agagttcaac aatgttccta tagcattaga tacagctaat
    13681 aaaaaagcta tcgaagctgg acttaaagtt tacgatagaa ctaatgcaaa accaataata
    13741 aactctgctg atgctggttc aagatttgat ttaatagata tagcagctga atatgaagca
    13801 atggttatag gtttatgtgc aaaagaaggt atcccaagag ataatgatga gcgtatggct
    13861 tactgccaag aaatattaga aaaaggctta atgttaggaa tggagccaac tgatatacta
    13921 ttcgacccat tatgcttagt tataaaaggt atgcaagaaa aacaagtaga agttttagaa
    13981 gctataaaaa tgatgactga gatgggactt ttaactacag gaggattatc taatgtatct
    14041 aatggatgtc ctaagcatgt tagacctgtt ttagatagtg cattcttagc aatggcaatg
    14101 gccaatggat ttagttcagc tataatgaat ccatgtgacc cagaattaat gaaaactgta
    14161 aaatcttgtg acataatcaa cggggcatct ttatatgcag actctttctt agagctaaat
    14221 gaaggtggat ttgctttcta gggtttttag ggtatagtta ataaaaaata ggtgtaaata
    14281 ttaaaggggg aaatagaatg aatctatata atataatctt tacagggtca gaacaagctt
    14341 taggtgcggc tcaagctatg ctagctgaag ctatagaaaa aaatggaaaa gaacataaag
    14401 ttgctttccc tgatacagca tattcattac cttgtatata tgctgcaaca ggacaaaaga
    14461 tgaacacttt aggggactta gaaggtgctt tagaagtagt aaaatcttta ataaatagaa
    14521 ctcatttact agaacatgct ttcaatgctg gcttagctac agctttagct gcagaagtaa
    14581 ttgaagcatt aaaatattca actatggatg ctccatatag tgagccatgt gcaggtcata
    14641 taactgaccc tataatcaga tcacttggtg taccactagt tacaggagat atacctggtg
    14701 ttgcagttgt tcttggtgag tgtccagatg ctgaatcagc agcaaaagtt ataaaagatt
    14761 atcaatctaa aggtttgcta actttcttag ttggtaaagt tatagaccaa gctatagaag
    14821 ctggagtaaa aatgggtctt gaactaagag ttatacctct aggatatgat gtaacttctg
    14881 ttattcacgt tgtatctgtt gcagtaagag cagcattaat attcggagga ttaactcctg
    14941 gtgatttaaa cggattatta gagtacacag ctaatagagt tcctgcattt gttaatgcat
    15001 ttggaccatt aagtgaatta gttgtatctg caggtgctgg agcaatagct ttaggattcc
    15061 cagttataac tgaccaaaca gttcttgaag ttccaatgaa cctattaact caaaaagatt
    15121 acgataagat agtagctact tcattagaag ctagaggaat aaaaataaaa gttactgaaa
    15181 tacctatccc agtttcattt gcggcagcat ttgaaggtga gagaattaga aagagcgata
    15241 tgtttgctga gtttggtgga aacagaacag aagcatggga gcttgttgtt aagaaagaag
    15301 caacagaagt tgaagaccat aagatagaaa taataggacc aaatatagat gaagttgatg
    15361 cagatggtgt attaagatta ccacttgctg taatagttaa aatagctggt aaaaatatgc
    15421 aagaagattt cgagccagtt cttgaaagac gtttccacta cttcttaaac tatatagaag
    15481 gtgtaatgca cgttggacaa agagatatgg cttgggtaag aatctctaaa gatgcatttg
    15541 ataaaggctt tagacttgag catataggag aagttttata tgctaagatg ttagatgaat
    15601 ttgaatcagt tgttgataag tgtgaaataa ctataattac agatgctgaa aaagtttctg
    15661 aattaaaagg tgaagcaata gctaagtaca atgctagaga tgaaagattg gcttcattag
    15721 ttgatgaaag tgttgatact ttctattctt gtaacttatg tcaatcattt gcaccagctc
    15781 acgtttgtgt tgttactcct gaaagacttg gtctttgtgg agcagttagc tggttagatg
    15841 ctaaggctac taaagaatta gacccaacag gtccttgtca accaatagaa aaaggcgagt
    15901 gcttagatga tagaacgggt gtatggaact cagtaaatga aactgtaaac caaatatctc
    15961 aaggtgcagt tgaatctgtt acactatact ctatattaga agacccaatg acttcttgtg
    16021 gatgcttcga atgtatctgt ggtataatgc cagaagcaaa tggatttgtt gtagttaaca
    16081 gagaatttgc aagtgttact ccagttggta tgactttcgg agaacttgct tctatgacag
    16141 gtggaggagt tcaaactcca ggatttatgg gacacggaag acatttcata tcttctaaaa
    16201 aattcgctta tgcagaaggt ggaccagaaa gaatagtttg gatgccaaaa gaattaaaag
    16261 attatgtagc tgataaatta aatgctacag ttaaagaaat gactggaata gaaaacttct
    16321 gtgatatggt ttgtgatgaa actatagcag acgattctga aggagtatta gctttcttag
    16381 aagaaaaagg tcatccagca ttagctatgg aaagtgtaat gtaattgatg gaaaatttaa
    16441 aataatatga tataatatta atatgattta agtaatttaa tcaataatat aatattgggg
    16501 gtctatcgaa atgaaattat taccagaatt aaaatactct aaggatcatg agtgggtaaa
    16561 agtaattgat ggagatgttg tatatatagg tataacagac tatgctcaag atcaattggg
    16621 agaaatattg tttgttgaaa caccagaagt agaagatact gtaactaagg gagtagattt
    16681 tggagtagtt gagtcttcta aagtagcttc tgatttaata tctcctgtta atggtgaggt
    16741 attggaagtt aatgaaaaat tagaagatga gccagaatgt ataaatgaag acccatatga
    16801 aaactggata ttaaaagtaa aactagctga tgtggctgaa cttgatacgt tattaagtga
    16861 taaagaatac gaggctggat tagaataatt aaagattagg atattattat aattggggaa
    16921 agtatagtaa aatttgagtg cacgcaaatt tagctatact ttccttttat catatacaga
    16981 acaaattggg agggataaca tgataaaagt gagttttaca ccaaacaata aagaggttta
    17041 ttgtaatgag ggggatatat tactagaggt tgcaagaaat gcagatatat tcatagatgc
    17101 tccctgtaat ggaaacgtgt cttgtggtaa gtgtaaagta aaattattga atggaaaagt
    17161 tgatacagaa aaaacaagac atataacaga tgatgagtgg gagcaaggtt atatactagc
    17221 ttgttgtaca aaggtaatat cagatataga aattgaagtt ccttctaaag tatcatcttc
    17281 tatgcatggt atgaagatag aaggtagtaa taaaaaagag gatagagaaa tatttgaaag
    17341 agctaaaaaa ataattgaag aacataacct tcaatttaaa acaaatataa aaaagaaata
    17401 tatagaaatg gaagaaccaa atttagatga taatataagc gacgtagata gattggaaag
    17461 atatgttaga aataacttag ggtacaatga aatagatttt agattggata tacttagaaa
    17521 gatgccaacc atatttagaa agagtgattt taaggttaca ataacatatg ttcaaaaaca
    17581 aaagaaactt acaatcataa atatagaaga aggaaataaa gaaaattcct tatatggagt
    17641 tgcaatagat attggtacaa cttcagtagt tgtttgctta gtagacctat actcaaaaga
    17701 ggtagtagat aaagcatctt ctggaaatgc acagataaaa tacggagcag atgttataaa
    17761 tagaattata tattcaacta agaaaaatgg attagagaca cttcacaaag ccatagttga
    17821 ggaaactata aatccactgc taaaaacaat atatgagaga aatggaatag ataaggagga
    17881 tgtagttaca ttagtggctg ctgggaatac aactatgaca agtttattct tgggtgtata
    17941 tacagacttc ttaagacaag agccttacat accaccattt ttaaaatctc caaaacttat
    18001 gggagaaaat gttggtcttt ttgtaaatga cagtgcatat gtgtacttag ctccatctgt
    18061 tgcaagttat gtaggaggag atataacagc aggtgtatta tcagcaggta tatggtcaag
    18121 tgaagaaaat gttttattta tagatttagg aactaatgga gagatagtat ttggaaataa
    18181 agattatatg atgagttgtg catgttcagc aggtcctgcc tttgaaggtg gaggtataag
    18241 ctgtggaatg agggcatcag caggagcaat agagaaagta attattgata aagaaacttt
    18301 agagccaaca ttaaaaatta tagatgaatg tgcaccagtt ggtatatgtg gttctggtat
    18361 aattgattta atctgtcaaa tgattactaa aggtgtaata gatagaagag gtaaaatata
    18421 cagggattta gataacaaga gagttagatt taatgagcat gagataggag agtatgtatt
    18481 agcatttaaa gaagaatttg atttagaaaa tgatatagta gtcaatgaag ttgacataga
    18541 taatttcatt agagctaaag gagctatata ttcaggtgca tacactcttg tagacagttt
    18601 aggtatggat tttagtatat tagatagggt ttatatagca ggtggtatag gaaataacct
    18661 agacatagaa aactcaataa ttataggttt acttcctgat attgatagag aaaaatttac
    18721 ttatatagga aatagttcac ttgttggttc ttatttagct ctcataagta aagatgcaaa
    18781 aaataaactt gaagaaatag gtaatcaaat aacttatgtt gaacttagtg tatatccaag
    18841 ctatatggat gaatttattt ctgcatgttt cttaccacat acgaatatag aacagtttcc
    18901 aacagcaaag aaattattag aagagtaaaa tgaacttaag aaggaataaa ctatgaataa
    18961 aaatctatct gaaaaagtga aagtagcatt tgatattaaa ttaaaaaatt ttggaaataa
    19021 aatagagttt gcttatccta atcagacact agctcttagt accacaaaca ataaatgtag
    19081 tttaaaatgt gctcattgca atggtcacta tttaaacaat atggtaccaa tagaggatta
    19141 tgaggaaaaa gtacaatcaa gaaatataac tagtttttta ttaagtggtg gatgtagcta
    19201 tgatggtgat gtcccaatga gtactcatat tcagactatt aaaagattaa aggagcaagg
    19261 ttacagattg aatgctcatt taggacttat ggataaagag agcattatag aattgtgtaa
    19321 gtatttagat atagtttctt ttgatttagt atttgataaa gaaacaataa gagaagttta
    19381 taagatggaa aagagcaaag atgattatat agaagtttat aatacaataa gagaaaatac
    19441 agaagtagca ccacatatat gtattggttt aaaaggagga cagataaaag gtgaatatga
    19501 aattatcgaa tacctacaga aaaatcctcc taataaactt acatttataa tccttattcc
    19561 aacaaaagga actgagtatg aaaatgtaga acctcctgaa ttagaaaggg tagcagatat
    19621 tttatgtgaa gctagaatta atttaccata tactgaaatt aatctagggt gtatgagacc
    19681 aagaggagtg tatagaaaag agctagacca gttatctata atgtgtggag tcaataaaat
    19741 agtattacca tcaaggtctg ccaagaataa agctatagaa atgaatatga caataactga
    19801 atgtaaggag tgttgcgttt tatgattaga ctatcagttg gaacagcaat agaacttgga
    19861 atattaaaca aaaaaagcga tatacctcct actacagcat atataatgat tggagaaaaa
    19921 tgtataaata aatgtagttt ttgctcccaa tcaatagaaa gtagtactag aaaagataaa
    19981 ttgtccagag tagtttggcc agagttttca aaagaggaaa tattagatgc gttaaaagct
    20041 tataaaggaa aaaatataaa gagaatatgt attcaatcaa tggcaagtga agaagcacac
    20101 aactctgttt tagattttat aaactatata agtggcaaga tagacatgcc aatttcacta
    20161 tctgcaaaat tagaaaatga tgaacagata aataagtttt tttcagcagg agtagataaa
    20221 ataggaatag caatagatgc agcaaataaa gaactatatg aaaaaataaa aggcaataat
    20281 tataatgaaa agttaaaatt tataactgaa atgtcaaaat catatcccaa taaaataagt
    20341 acacatataa tagttggtat gggtgaaagt catgaagata tatacaattt atatacttat
    20401 ttaaagggaa atgacataac gattagttta tttgcattta ctccagttag aggaactaaa
    20461 atggaaaaga taaatcaacc aaatatagaa agctatagaa gggtacagtt aatgtcttat
    20521 atgataaata aaggttattc aaaagagtat tttacgttta aaaatggata cttagatagc
    20581 ataaagcttg acaatgatat tgtaaaagac ataaacaaag gttatccttt tgaaataaga
    20641 ggttgtaagg attgtaatag accatactat aatgaaagac caggtagtac tatatataac
    20701 tattcaagac ctttaaatca aagtgagata gatttagcaa taagagaaat aaatctatag
    20761 aggatttcta ggatatattt atactaaaaa tgggggcgta ataatcatga atcagtggag
    20821 agttatacat aataaatcat atgaaggagc aatgaatatg gcaattgatg aagctatatt
    20881 tactgcatat aagaagggac ataataagcc aactcttaga ttttatacat gggaaccagc
    20941 ttgtctgagt ataggatatt ttcagaaatt ggaagatgag atagatttgg acaagtgtag
    21001 atgtatgaat atagactata ctagaagaat tacaggtgga agggctgtat tacatgataa
    21061 tgagctaact tatagtataa ttataggaga agataatcct ttaattgata agagcatcaa
    21121 cctgtcttat agatatataa gtgaaggtct agttaaagga ctgaatttaa gtggtataga
    21181 aacagataat ttgaatagag gcgagagaat aagtagggaa aatttatcag ctgcgtgttt
    21241 caatgcacat gcatcatatg aagttaccat taataataag aaagtaattg gaagtgctca
    21301 aagcagaaaa gatggtgttt tattacaaca tggttcaata atactggatt ttgatgtaga
    21361 aaaattgttt aaattaataa aaacgaagac accagaactg aaagaaaggg ctatgaaatt
    21421 tacagctaaa aaagcaagtg gaatagaaaa tgaaattggc agaaaaatag acatagatat
    21481 tctacaaaaa aacatagtaa agggattagc tgaacaattt aatgttgagt ttgtggaggg
    21541 tgatttaact gattacgaga agcaattagc taaagaacta tatgaaaaat acaagaatga
    21601 agagtataat aaaaagcgtt aactttaatt gactttatat aaattaaagt gctatactta
    21661 aggtataata atatacaaaa actgaaatca tgaaggtttc tttggtgtat tgaaatacct
    21721 aaggaaatct ttttttgatt tttaataaaa atcatattag gaggagattt atatgtgtga
    21781 atcatcagct ttcatttgta aaagtaataa tgagttagaa aaggttatgg aaaatgtggt
    21841 taatatagac ccttgtgatg gaaagattta tttaacggat ttattaggag agcaaaagat
    21901 aatagatggg ataattaaag aaataagatt gatggaccat aagataataa tacaagaaaa
    21961 ttaattaata ggggaatttc atatgataat agcagtgata gatggaatgg gtggaggtat
    22021 tggagcccag atagtttctt ctttgagaga agagttacct acatatgtag aaatttatgc
    22081 actagggact aattctatag caactagttc tatgatgaag gctcatgcta ataaaggagc
    22141 tacaggggaa aatgctatag tagtatcagc taaaaaagca aatataatag ttgcacctat
    22201 ttctgtaata atacctaatt ctatgatggg agaagttact tgtaatataa gtgaagctat
    22261 tgcagatagt gaggcactta agatactttt acctattatg ccagaaaatg tggagttagt
    22321 tggattagaa ggtaaaccat tagcactact tgtaaaagat tctgtaaatc ttataaaaaa
    22381 agagtttaat ataaaataac agggggaata actaatgatt tttgatagag agattgtaag
    22441 atatcatcat ggacatcatg accatgacca ttgccacgag catactcatg gagatatatg
    22501 tcatgaacat ccacatgacc atgctcatga ccatgaccac gaacatagtc atgaggaatc
    22561 atcagagtct aaggatgaaa aaactttaaa aatacttttg gtacattgga taaatcataa
    22621 tgaaactcat gaagaaggat ttagagagtg ggttgaaaag gctagagcta ttggaaaaga
    22681 agaaacagca aagagtattg aaaaggctat agagtatatg gaagaagcta ataaaatgtt
    22741 gttagaggct aaaaaacata tgtaaatata attaatcgct tatacgtttg atataagcga
    22801 tttttatgtt ttttgatgct taattattaa atttcttatc taatggattg tctcaaaata
    22861 gatataagcc tctctttcat tgttgaaatg ataaaaatat actttaaagg ctgtttaaaa
    22921 ttgctccaag attagatata ataagaatta gtataattaa aaatattgtt ttgaagttaa
    22981 aaaatttatg tatttatata agattttttc tttaaaaaag gagtgaatta tttatgaaag
    23041 atggtcatat acattcacct tattgtcctc atgggagtaa ggatgatttt gaaaaatacg
    23101 tacaaagagc aatggatatt ggaataacag aaatgacctt tacggaacat tttccatatg
    23161 caaatggatt tagagaccct gctcctgaaa atgatagttg tatgagtatt gaagatttgc
    23221 caaagtattt taatgatgtc aagaagttga aagaaaagta tgagggtaaa atcaagataa
    23281 atgttgggtc agaagtggat tttatagaag gatacgaaga agggataaag gcgaatttaa
    23341 ataaatatgg agaagaatta gaggattctc ttttatcagt acatataatt aagattgatg
    23401 atgattatta ttgtgtagat tacagtatag aagaatttca aaatttaatc gacaagctag
    23461 gtagtattga agctgtttat aataagtatt atgaaacact gataaaagct gtaaattcag
    23521 atttaggtat acataaacca aaaagaatag gtcatttaaa tttagttaga aagtttaatc
    23581 aggtatttcc atacgattat aaaggaaata ccattcttga agatttaata aagttaatta
    23641 aagaaaaagg ttatgaactt gattacaatg tttctggaaa tagaaaagag tactgcaaag
    23701 agccttatat agatggatac ttattagagt tggtaaaaaa atacgatatt ccattagttt
    23761 taggctctga ttctcactgt gcagaggaaa taaataaata cgatattgtt tagatatatt
    23821 tgcataaaag actgtagata agtttgaata aaataaattt atctacagtc ttttgtaaat
    23881 ttatatgatt aaaaatagta atagaactta aataaaataa taatgagttc taagtaaaat
    23941 aacaatagaa tctaagtaaa ataataataa taaaattcaa tagaataata ggatttagaa
    24001 ttaatctatc ttatttatat aaacattctt atataagatt gtagataagc ttgtatttac
    24061 acaaatctat agatattctc tattatattg acttagtaat tataagtact attttgcatt
    24121 taaagttatt atttcataag tttatcgttt tccattatta cagggtcagg caattcaatt
    24181 tcactttctg acatcttcaa tctattaaac atataatttt ttacatcttt cttgttttta
    24241 aatttagtaa cttgataagg tgatgtaggg ccgtattttt ctacaaatag aatgccattt
    24301 ttagttttaa tacatatgcc tgtatgacct ataaatacgt ttttagtttc tggataacga
    24361 agaaatccat ttataataga tatattttta ttatttttaa aagatatttt tctttttttc
    24421 cactcatttt ttatttgttc tgcaatttta gatatatcac ttgtatcttt agctggtatt
    24481 gcagaaaaga agttgataaa tttttctaca tctttggtgc taaatttagc atctttatta
    24541 ttcattatag tgtccaagtc gacatttaaa tcaatagagt ctccagtaaa tttattattt
    24601 gaatttataa aatctttaaa aagtctaaaa gcagttattc tacaatttac atccatataa
    24661 tcagggaatt ttttcatcca attatcttgc aatttttcta ggttatatgg aacttgtttt
    24721 gcattgattg aagaatatcc agatttagat atatttatat tattttgaag tattttaact
    24781 tgcaatttat tataattgtt gactgcttta ataaatgtat ctatgtcttt ttcattaatt
    24841 ttattttctt ttagaatttc ctctacacta ttttgagttc ttttatctat catattagaa
    24901 tatatcaatt tattattata tttaatattt gaatatgcaa atatcgtaag tacacttgat
    24961 agtactaaga caagagataa tataattatt aatctttttt tcattttgag ttctcctttt
    25021 taaatttatt gtgtcgacat atttatagta tatagtacct ttaaatttac cttcaatatg
    25081 tttttaatta caaaactgtt gtaaaaaata taacgttatt taaaactttt ttttaatatg
    25141 taaaaatata tactgatagg gggtatttgt ttttagaata ttcagtaaaa tagttttgag
    25201 atattatata tattttgtat gttaaaaata aagaaattat tgttaaaaca gataaagacc
    25261 ttaaattttt tatcgtaagc ttttaagtaa ataagacgta ttgaactaat taaaaatttt
    25321 tattttgtaa aaatgagtat ttatttatat ggataaattt gattttaaat ctatcaatat
    25381 agataaaatc tcattattta acttcaacta ttcctcaaaa aagtaacttt taaggaatag
    25441 ttgaaacaaa aagtttggtt acataatagt aattttagtt acaagaatga aactgtaata
    25501 cttaggattt tctttatata atttcattga gaacatttaa cttaggaggt aagatatgaa
    25561 agtaaaaaaa tttaaaagtg gtatagtaat ggcattaaca gtaatggtaa ttggagcatc
    25621 attgacaaat gtatcagcta tggaattaaa taagagtaag gagtttgaaa ctaaatacaa
    25681 tgaaatgaag agggattttc aagaggaata tggaaaagaa gacaaattta atataacatt
    25741 ggatgatgaa tttacaaaag aagaagtaaa tggagctaaa gggattataa agacaactaa
    25801 taaagtgaat aatgaggttg tagtattaaa ttattttgat gaaatgaaat ctgggattga
    25861 atttgagttg aagccaaaag cagaaaaaaa tgtaattttg agagaggaat ttcaacaagg
    25921 ttatggaaag gaagataaat tcaaagttgt gttagatgat gaatttacaa aagaagaggt
    25981 aaatggagct aagggaatta taaagactac agataaagaa acaggtgagg ttatcgtata
    26041 taattacttt gatgagcttg aaaaataatg tgtaaagatt actttgattg aatgcaaagg
    26101 taattgtttt gagaatatga aatgactgaa taaaaaacga ttgtatcaaa aacaaataat
    26161 aattgaatgt aagtataatt gtatcaaaaa tataaaataa attaaaagaa aaagattata
    26221 ctgaaggaaa aagatttgaa ataggggtga actttacata ttatagttca cccttataca
    26281 aaaatataaa atataattta ttataggaaa atttaaaaaa atcagattat ttaatttata
    26341 ctgatatgtt gtaggatttt acaaacaaac tgttatataa ctttttttat ctccaatctt
    26401 gattttaata tatctaagaa tttataacaa tcttctggat atatttctcc catatttgca
    26461 acttggaata tgttagcatc taggaatttg ccttttcctg gatatacaac atagccactt
    26521 tccctttcta gccctaatat aaactcatct acattgattt ctttaggtag aaatacagat
    26581 gttactgtat tagacatata cttatctgga agtaaaaatg ttagacccaa ttcttccatg
    26641 ccattcctta aaatagcaga acattctttg tatctttcta ttttactgtc atcttccaaa
    26701 aactcagtta aagcttcatt aagggcaaat attaaagtaa cattaggtgt atttggtgtt
    26761 tgagaagatg atttagcaac tttatagtgt ttataaagat taagatacac attcttacat
    26821 tgattttctt taatatcttg taaaatactc tctttagcac aaaggtatgc agaacctgga
    26881 aatgctccaa gagctttacc accaacacct gttattatat ctatattaaa ctcttccaca
    26941 tcaatatatt cacctgctga tgcacttatt gcatctacaa aaaatatttt gttatacttc
    27001 ttagcaagtt ttcctagttc aggaaccgga tttatcattc ctgaactagt ttcatggaac
    27061 accatagcaa ttacttttat atcagaatgt tcaattatat atttttctaa aatgtttagg
    27121 tctgggtatt ctgcccattc aaaattaggc ttatatgttt ttacattata agtagataat
    27181 atttcttcta gtctttctcc aaaaacacca ttactaacta gcagaactgc atcttcttta
    27241 tcaaagatag aagatagtac acattcattt acagatgttc cagagccaga tacaattaat
    27301 gagtagtaat tagaagttgc attatatagt ttattgattt tttgttgtgt atctttgaat
    27361 aaatccataa actcatttcc tctatggcat atatcatagt gaagaagtga atttctgact
    27421 ttatctgtaa ccattactgg tccaggactg aatagatatg tttttttcat tgtaaagcct
    27481 ccttaaaaag ttaaatttaa atttttgaat gactgattat gtgtaaatat tagaatatta
    27541 tgtgttattt taactagatt atagaataaa ataataaata ttgcaataat attttaataa
    27601 aaatactttt ttaatcatgt taattaaaaa taacaaaaaa tattgtaatg aatattgcat
    27661 aaatccacat gatgatataa aatataaata acttgaatag attatatatt caaaaaataa
    27721 aaaagaattt attaattctg aaaataaagg gggacattta aagtgaaata tgtattagca
    27781 attgaccaag gaacaacagg aacaagagca gtattgataa acgaaaaatc agagtttgtt
    27841 tttagcgatt atatggaaca tactcaaata tatccacaat ctggatatgt cgaacatgac
    27901 cctatagaaa tttggaataa tacaaaaact gtgagtaata atgtattaca acaggctttt
    27961 gaaaatggaa tcaatgaaag cgatataaaa ggaattggta tagataatca aggtgaaaca
    28021 gtaatgttat gggataaaaa tacaggtaaa ccactttaca atgctattgt atggcagtgc
    28081 aggagaactt atgattatgt ggaaaattta aagaatatac caggtttaga aaataagata
    28141 atagataaga ctgggttaat tatagaccca tatttctcag gcacaaaaat taaatgggta
    28201 atagataatg taaaaggtgt aaaagaaaaa attaaaaatg gagaagtact tgcaggaaca
    28261 ttagatagtt ggttaatatg gaaaatgaca ggaggaaaat cttttttaac tgatgtatct
    28321 acagcagcaa gaacaatgct ttttaatata agagatatgg attgggataa agagttgctt
    28381 gacattatag gagtgcctag aagtatttta gcagatataa tgcctacaag tggtgatttt
    28441 ggaactacag ataaggaggc attttgtggg gtttctataa aaattactgc ttcaatggta
    28501 gaccaaccag cagctttatt tggcaatgga tgttttgaac caggaatggt taaaaatact
    28561 tatggtacag gatgttttct atatatgaat ataggtgagg aactaaatat agcaggtaat
    28621 ggcattttga caactgttgc ttggaaaata ggagataaag ttacatatgc ttatgatggt
    28681 ggagtgtata tagcaggtgc tgcaatacaa tggcttagag atggaattgg agttataaaa
    28741 aattattcag aaactgatga catggcaaat tccatatcaa gtactggagg tgtatatttt
    28801 gtaccagcat ttgcaggtat agcagcacct tattgggacc aatatgctag aggtacaatg
    28861 gttgggataa caggaagtac taaaaaagag catatagtaa gagcgactct tgagtctgtt
    28921 gcacttcaag tcaaggatgt tgttgattct atgaacttag cctcagggaa aaaaataaaa
    28981 atgttgagag tagatggtgg aattactaaa aataacttta caatgcaatt tcaatccgac
    29041 atattaggca taccaattga aattgcagaa aatcccgaga caacagtact tggagtagga
    29101 tatatggcag gtctttcctg tggtgtctgg aacagtattg atgaaattag aagtgaattt
    29161 acaataagca aaaaatatat tccaaatatg actaatcaaa aaagagaaga aatattactt
    29221 ggatggaatc aagcagtaaa aagagctatg aattgggaaa acgataccaa aaaagtgtgt
    29281 gctaattaag ttacaataga gtattttaca tgtataggag gaattaaaaa tgccctttga
    29341 taaaattata ttatacataa tggctgtggg agtgctttta ggagcgttag atagaataat
    29401 tggaaataaa tttggacttg gagagcagtt tgaagaaggg tttaattcaa tgggtccatt
    29461 ggctttagga atggttggta tagtttgttt agctccagta atttcagatg tactaggtcc
    29521 tataataata ccaattttca atctctgtgg agctgaccca tctatgtttg caactatttt
    29581 agcgaatgat atgggagggt atccattggc tatggaactt gcacacaata aagaggcagg
    29641 aatgcttgca ggattggtcg tgtcatccat gcttggatgt acattagtat tttcaattcc
    29701 agtaggacta ggtcttatag aatatgaaga cagaccattc tttgcaaaag ggttactgat
    29761 aggcctgata acaatacctt ttggaggtgt tataggagga ttaatagcag gatttaactt
    29821 atcaatggta attataaata tgataccagt gataatattg tcagtaatat tggcagtagg
    29881 tttaaaattt aattctacac ttatgataaa tgggtcatta gtttttggaa aatttatatc
    29941 tatattgata acgataggtt tgggagcagc agcatttcaa gaaataacag gtatagtact
    30001 tataaaaggc atgacaccta taagagaagg gattcaagta attggaagta ttgcaatagt
    30061 gttattaggt acttttccaa tattacattt attagttaaa gttcttaata aaccacttac
    30121 aatagtgggt ggaaaattag gtatggatgc cactagtgca gcaggattag tttttacttt
    30181 agccaatagt attccagtat ataaaatgat gaaagatatg agtccaaagg ggaaagtagt
    30241 aaatactgct tggttggttt gtgctacagc agctttggga gaccatttag gatttacagc
    30301 aggagtagtt ccagagatga ttacacctgt tgtaattgga aaattggttg gaggagtatt
    30361 ggcaattgca cttgccatac ttatgacaaa agatttaact ttagaagaag aacaaagtat
    30421 taaaatgaag aaagatgcag tgtagaataa ataataatta agtcaaattt aaactatttt
    30481 aaagtaggag tttaaataac taaaaataaa ttaaaaagac agacaattaa aaaataatcg
    30541 aatatccaaa acttataaat taaataaaat ggcaatcaaa taactaaaaa tgataagtta
    30601 actgaaagta attacataat taaaatataa agtatagttt agacataaat ggagctaaat
    30661 aaataattat ttggctcctt aattttaatt tattacatca aggaactaaa aatcaatcat
    30721 acaaactaat tatcagctat gactaaaggt attccttttt gagaaaactc tattattata
    30781 ttatggtcta tactagcaga ggtaataata gtatctatat gttctatagg agctactgat
    30841 gcaaatgaat ttataccaaa cttaggaggt tcgacaacta ctatagattt tctagacctt
    30901 tcaatcattt tttttctaac aagtgcttct tctaagtgat agtcagagat tccattttca
    30961 agagtgatac ctccagcacc taaaatagct ttatcaacat taaattgttc aatagctgac
    31021 agtgtaagcg gaccagaaaa agaaccttca gtttctctaa gatagccacc aagcacatat
    31081 acttttatat tagtattcat aagttcgttt gcaactaaaa ttgagttggt tattacagtt
    31141 agattctttt tatctttcaa attttttgaa acttcaagta cagtagttcc agtatccata
    31201 aaaatagtat catttgtatt tataaactca ctacatttaa tagcaatctg ttttttatcg
    31261 tttgagttta ttttttttct tttaacatag ttaagttcta cgctacttgc tataactggt
    31321 atagcaccac cataaactct ttttaaaaga ccgatactct ccatatgggc taaatctctt
    31381 cgtgcagttt ctattgatat atcaaacatt tgaactatgt catttatttt aattatatta
    31441 tctgatttga gaatacttaa aatcttctcg tatctttcct gtggtaacat aaaatatcct
    31501 ccttggacta gtattatttt agttttggca taacttacaa ttaagtcaga gtttaggtat
    31561 atttgttaat gtatgtatat ttatcaaata atattataag tgttatgcaa gataatttta
    31621 ccatatttaa aacttacatt taagcaaaaa agtaaaaata gatgtttaat aattcaaaat
    31681 aactcaaatt ttataaaaca attgactaaa atatgatttt attatataat ttattagtat
    31741 agagatatgg agaagtactc aagtggctca agaggatccc ctgctaaggg attaggctgg
    31801 gtaaccggtg cgagggttcg aatccctcct tctccgccaa ttgttaaagg ctatttttat
    31861 taaagatagt ctttttatga tgtcataaat gattagatat gtataactaa agaaaggagg
    31921 gaatagatga caactttaat ttttttcata gtggggatag catctttggt agttgctttt
    31981 gtaattgatg gggggcatgt tggagcgtta tttgtatttt catcagcact cgttgttttt
    32041 ggtggaacta taggagctgt tggagcttcg acgccattcc ctgtatttaa aaagtctata
    32101 aagataatgt tattagcttt taaaaataaa aagcctaata acatagaaaa tataatctat
    32161 ttcaaagaaa ttgcaacaaa ggctaggaaa gaggggcttc ttagtattga caaggaaata
    32221 agtgataata atgaattaga cccttttatt aaaaaagggc ttgaactgat ggtagatgga
    32281 gtggatgcag ttactataag aagcatattg gaatcacaga tacagataac atcaaaacga
    32341 cataaagaag gaattgcaat ttttgattca gcaggtggat atgcacctac aatggggata
    32401 ataggtacag ttatggggtt agtacatatt ttaggtaatc tttcaagtaa tccagatgca
    32461 ttaggacctc aaatttctgt tgcatttttg gctactttgt atggtgttgg ttctgccaat
    32521 ttaatatggt taccaatagc atcaaaactt aaggctataa atcaacagga aataagtgaa
    32581 agacttttgg tgatagaagc aatattatcc atacttgagg gtgataattc taatttaatg
    32641 acagaaaaat taaaaatatt tttaaattca aaagaactaa ttgaacttga agaaattggt
    32701 ggtagagctg aataatataa aaggatggaa tgtttatgtt gtatgatgaa gatgaaaaag
    32761 aagaaaacaa tgagcgatgg cttttaactt attcagacct cataacatta cttatgattt
    32821 tctttgtaat aatgtattct atgagtaatg ttgatgcgga gaaatataaa caattatctc
    32881 aatctctaaa ttctgctttt ggtggttctt ctggagttat tgaaggtggt aatagtaaaa
    32941 tagaaccagt agttgaacca ggaagtaatg atttagataa tttacaaaat gctaagttta
    33001 aaaaagttgg tgaagaaatc cagaagtatt taaatgaaaa tggtatggct aattcagtat
    33061 cattaagagt tcaagataga ggattagtaa taagtcttaa agatacaata ctatttgata
    33121 cggggaaagc agttgtaaag gacaattcca gagagaagat tattcaaata ggtaagatgt
    33181 taaatgaaat gaatagttac attagagtag aaggtcatac agacaatatg tctataaaaa
    33241 attcagagtt taagtcaaat tgggatttat cagttatgag agcaaccaat gttgttcaac
    33301 ttcttattga caatgcaggt atagcaccag ataaattgtc tgctgtagga tatggtgagt
    33361 ttagaccgat agcagaaaat tcatctgaag aaggtaggtc aaaaaataga agagtcgata
    33421 tagttttagt tgatagtaaa tatgataatg tagaaaatgt ttcaaatgat aaatagttaa
    33481 attagctgtc tcaacacaga attaattcta aaggggatgg cttttttatg cgaaaattaa
    33541 aaatagatag atagtaagtt acctttttgt ggtaatatat ggtatcttaa atatataata
    33601 tttaaataat atataagctc aattaaaaaa attttataga ggatgggaaa tgaatgggaa
    33661 ataaaaatac gaaatataaa gataaaacaa gagaaaggta taataaaaaa gttgagaatt
    33721 taaataaaga aaaattaaat aaagctaata aagaatctaa gaaagtagat aagattgatg
    33781 atattatcaa taaatctagc attgtattat gtgtacttct agtagtgttt tcattacttt
    33841 acttattcaa attagttcaa cttaatataa taatgatact tttagtaatt acagtagcta
    33901 ttcaacaggg attggtgtca tttggtgcat ttagaaataa aaattatgta aaagctttat
    33961 tttctatatt tgtaattgta tgtttcttaa ttgcttttaa atatgttgta aatcctgttt
    34021 aaatatatca ataaacgttc tataattaat ataaatagtg aagttatagt tttaatgcta
    34081 ttgtaaattt tataatttga aggagtagat tttatgaata ggtgtaagtt tgcaaaatta
    34141 attatcacat taatgatagt tttattactt acaggatgta gtttaaaaaa ggaagataat
    34201 tataagctag acccagaaaa tccgatatca atacaaattt ggcattatta taatggacag
    34261 caaaaaatgg cttttgataa acttgttaag gaatttaatg acacagtagg ttctgaaaga
    34321 ggaattattg ttgaagcatt cagtcaaggt aatgtagttg atttatcaga gaaagtttta
    34381 gattctgcaa ataaaaaagt tggctctgaa gatgtaccaa atatatttgc agcatactca
    34441 gatactgcat atgaaatgga taaattaggg ctactagtag atatggataa gtacataagt
    34501 aaagaagaat taagtgaata tatagacgaa tatgtagaag aaggtagaat aaattctaag
    34561 ggtgagttta aacttttccc aattgcaaaa tcaactgaaa ttttgatgtt gaataaaact
    34621 gattgggata aatttgcaaa agctacaaat tcaaatataa atgatataaa aacaattgaa
    34681 ggattagtag agacatcaaa aaaatattat gaatggacag atagtttaac gaaagagcct
    34741 aatgatggta aagcgttttt tggaagagat gctttagcaa attatattat tataggaagt
    34801 aaacaactag gagtagaaat tttttctgtt aaagatggta aagttacatt aaatattgat
    34861 gaaaaagtta tgaaaaaact atgggataat ttctatgtac catatataaa tggatatttt
    34921 gcttcttatg gtaagtttcg ttctgatgat gcaaaaacag gtgatatatt agctcttgta
    34981 ggctccagtt caggagcagc atactttcca gatagagtaa tgctaaatga caatgagagt
    35041 tataatattg aaacatctgt ttttcaagcg ccacgtttta atggagaagg aaaatatgct
    35101 gtacaacaag gtgcaggaat ggctgtaaca aaatctacta aagaagaaga atatgcatca
    35161 gtagaatttt taaaatggtt tacagataaa gaacctaata taaaattctc tattgcttct
    35221 ggatacttac cagtaaagaa aaaaacgaat gatttagagg ttataaaaga agttatgaag
    35281 aaagataatt tgagcctatc tgataaagtt gaaggctcaa tagcttcatc tattgaacaa
    35341 attaacagtt gtaatttata tactaataaa gcatttgatg gtggaactaa agctagacaa
    35401 atacttgaat attcaatgct tgataaagca aaagaagatt taaataaaat aaatgaatta
    35461 gtaaaaagtg gaacttctaa gaaggatgca gtagcacaat ttaataatga taagaatttt
    35521 gaaacttggt ttgaaagttt taaggctgag cttgaaaaag ctataaaaaa atagatgaat
    35581 tggcagttag tgtgtcgaca agcacaaagg tataggtgga ataagtatgg aaaataatct
    35641 aagaaaaaac aattcaataa tgaaaaagat gatatttccc ctaattattg ttatgctaat
    35701 tcaaacaagt ttattttgtg ccacaatttt atggggagga actattaaga aattaaatga
    35761 taattcgttt gacatcttaa atgaaagagt aattaatcgt aaaaattata tacaaaatga
    35821 aatgttacaa agatggtcaa atacaagtga aacagaagct agaattaatt cctctgtaaa
    35881 aaaagtctta aatgaaaaaa atgctttaat tgaggatgta ggcaacaatg aagaaattaa
    35941 caaagacatt ataagaatca tatcaaatga tttaatatat cttttgagaa aaaattctgt
    36001 tacaggagct tttataatat taagtgacaa aaatattaaa gataatccag tagatagtaa
    36061 tggtgaaata agtaggactg gattatatat aagggattta gaccctaaat ttaatccaaa
    36121 tgataattct gatttgttaa ttgagagagg ttcatcagat atagcaagaa gttttggaat
    36181 atctatggac agttattggg aacctaaatt ttcatttaaa agtgaaaatg aacaagaagg
    36241 agatgatttt ttttataaac catttagagc tgcaattgat aatccaaatg tatcaagttc
    36301 taatttggga tactggggaa gtaagttttt attgacaaat gcaggtgata ggattatgac
    36361 atatacagtt ccacttattt atgaagatgg aactgtatat ggagtacttg gaatagattt
    36421 aagtgtaaat tatttaagag aattacttcc atactcagag ataaatggaa ataaaaaagg
    36481 taattatatt ttaggtgtgg atagaaatga tgatatggca tttgaaaatg taatttcaaa
    36541 tggatactca ttaaaagaaa agttttggaa tggtttacat tctaatttag agcagaagga
    36601 atcgtataaa aatatatata agattaaaaa taaagataat gaatgtggta atggcaatat
    36661 ttatgctagt gtacaatact taaatttata taattccaat actccatttg aaaatgaacg
    36721 ttgggctctt ttgggtatta ttgaagaaaa aagtctttta tctttttcaa aaagggtaga
    36781 gaaattagtt ataatatcta tagcaatttc tttattaata ggtctaatag gtatttatgt
    36841 tgcatctaga ttatttacaa aaccaattgt tcttttagcc aacaaagtta gaaacagtaa
    36901 tccatcaaaa ccagtaaatc ttgataagat aaatatatct gaaatagatg aattatcttc
    36961 agctatagaa ttactcagtg caaatgttgc agattcctca tcaaaactat cgcagattat
    37021 tgaaatggta gatatgccaa ttggggcttt tgaattatac aaaaatgcag ataatgtttt
    37081 ttgtacaaaa gaatttttta gtgtttttat tactgaaagc aaatttgaag aatatggtta
    37141 tatggaaaaa agtaagttcc ttaaaaaatt aaggggatta gataaatatt tgcaagaaag
    37201 ttatgaaaca gaaaatacat atatatacaa gtttgaatct aaagataaaa agcctaagtg
    37261 ggtaagactg aatatctttg aagatgagtt aaaggttctt ggcgttgttg ttgatgtcac
    37321 aaaagagttt attgagaaaa gaaagataga atatgagaga gattttgata ttttaacgaa
    37381 tcttttaaat aggagagctt ttcattcaca gatacaagaa aaatttaaga ataaagatga
    37441 acttaaggta gcagcattca ttatgtggga tttagataat ttaaagtata ttaatgatac
    37501 atatggacat gatgctggtg atgaatatat tagatgtgct gcagacatac ttaagaagtt
    37561 tatatcgtgg aatggtatag tatctagact ttcaggtgat gagttttatg tatttatata
    37621 tggatatgaa agtaaagata gtataagaga tattattaat tctgttaaga aacagatgaa
    37681 taatacctta ttaaaacttc atgatggaac agaatttaaa ataagggcat cagcaggaat
    37741 ttcctggtac cctgatgatt caatcaacta tgatgagtta atgagatacg cagattttgc
    37801 tatgtatcaa attaaaaata caatcaaagg tgaagttagt gaatttgata ttaatatata
    37861 taataaggat tcattcttat tgagcaatag agaagaatta aacaagttaa ttgatggtca
    37921 actagttgaa tatgcatttc agcctattgt agattgtaaa ggtaaagtat ttgcatatga
    37981 agcacttatg agacctaaaa taaaaacttt tagttcacca atagatgtca ttagattagc
    38041 tcgttcacag tctaaattat atcaaattga gcgtattaca tggtttaagg cacttaaatc
    38101 atttaaaaaa tacaaagaaa aatttggtga ttgtaaactg ttcttaaatt ctattccaaa
    38161 taataattta tcaaaagtag attttgagga aattgaagat ttatatggtg aatatttaaa
    38221 tagagtagta ttagaaataa cagagaatga taagattaat gaagatttta ttgagaaaaa
    38281 gaaagcatat atagaccatt ggaatataga aattgctttg gatgactttg ggacaggtta
    38341 taatggagat gctgtactat tatatataat gccagactat gtaaagattg atatgtctat
    38401 tgtaagaggt attgataaag atgaaaatag acagaaaata ttaaaaaatc ttatctcata
    38461 ttccaacgaa agaaatatta aagtgatagc agaaggtgta gagacaaaag gtgaaatgga
    38521 aacactaatt aatcttggaa tagattatat gcagggttac tatattagta aaccatcatt
    38581 tatacctgaa gatataagtg ataaaattaa aaaagagctc aagatatgtc atgaaaatag
    38641 gaatgatgtg gtttaataga ggaaaaatta aatttatttt tatgaacttt tagtttataa
    38701 ttaaaaagaa aataatataa ttataaatta aggtggtgga gctaataatc acaacaaata
    38761 tagatataag aaagttaaat gaaaatcaat tagaagcagt tgagcatata gatgggcctt
    38821 gcatgatact tgcagggcct ggttcaggta agactagagt aattacatat agaatagcca
    38881 atatggtcgt aaacaaaaac atagcaccaa caagaatttt ggcaataagt tttaccaagg
    38941 catcttcgat agaaatgaaa aatagagctt tgaatttaag tgatgatata agacttaata
    39001 aggtaaccta tggtactttc cactctgtat tttttaaaat tctaagatac tttgaaagat
    39061 ataatttaga tagtatattt gatgaaaaat caaagcgaat gacaataaag gcaatcttaa
    39121 aaagtttaaa tatagagaat gctgatgatg atgagaatat aggacaagtt ataaatgaaa
    39181 tttcctatgt aaaaaatgaa cttatggata aaaatgaatt taattcagaa gtattaacaa
    39241 aagatgagtt tttaaaagta tacaacttat atgaagaaca aaaatctaaa gttaataaaa
    39301 tagattttga tgatatgctt ataagaactt attatttact tttgaaaaat aagtctgcac
    39361 tagaaatggt tagaaatgtg tataaatata tattgataga tgaatttcaa gatataaata
    39421 aagtacagtt tgaagtttta aagttaattt ctagcccatt gaacaatata tttgcagttg
    39481 gagatgaaga ccaaagtata tatggattta gaggtgctag accagatttt ttgcttgaat
    39541 ttgaacagta ctttaataat actaaaaaaa taatattaga tataaattac aggtctaaaa
    39601 gtgagatagt gcatactgca aatagactta tcgacaaaaa taagaataga tatgaaaaag
    39661 ttattaagtg tagtcaaggt gatggaggaa gtgttaccta tatttctcca catgattcag
    39721 aggaagaagc cttatatatt gccaaggaaa taattgacga aataaaaaaa gattatgtag
    39781 agtattcaga ttttgcagtg atttatagaa ctaatataca gtcaagagca ttagtagatg
    39841 tatttatgga tatgaggatt ccatttgtag taaaagattc agtaattaca atatatgacc
    39901 attgggcaag tcaagatata ctagcttatt taagaatagg tattaatcca aaatcaaata
    39961 aagattggtt gagaattata aataagcctt ttagatatat atccaaagac agtataaata
    40021 tggttaagga tgaaaaagat tttataacag cattaataaa taagtgtaat cttcatccta
    40081 aacaagttaa aacaataaat gatttggaaa tagatttaag ctatttaaat actttaaatc
    40141 ctaaaaatgc aatatcttat attagaacta gccttgatta tgatagatac gttttagatt
    40201 attgtagcaa cagaaagata aagactaatg gacttgtaga gatattaaat gaacttgaga
    40261 gctcagcaac taattttaat actgttacag agtttttaga gcatattgat agagtaaaga
    40321 ctgagttaac ggaaaataac aagaataagc agactgaagg tgttatattt acaactatgc
    40381 atagtgcaaa aggattagaa tttagaaatg tttatattat aggtgtaaat gaaggaacaa
    40441 tacctcatga aaaatcatat gatatttgta aggaagagaa aaaagaagaa caattagaag
    40501 aagaaagaag acttatgtat gttgctatta caagagctga agaaaaatta tgtataagct
    40561 ctactttaaa taagtatggt aagaaagttg ataaatcatt atttattaat gatataaaat
    40621 ctccaacgaa aaaagaaatt gatagtattg gtgtagggga taaggtatac cataaaaaat
    40681 ttcatgaagg agaaataatt aaaaaagatg gaattatgtt tacaataaag tttaaagata
    40741 gagagcgaat cttggatttg aaaacttgtt tattgaagaa tataatttat actatttaat
    40801 ttagaaccta aatttagata caatgtatta tttatatgga tagactagta agaagtatat
    40861 tctttattgt gtgtaagtaa aagagatatg tgtaaaatgt attatttaag ttattagaaa
    40921 agagaaataa acgtttaata tattatttat agttgttaag aaaaagcaaa actgtgaaag
    40981 atattattta gcattattta agaaaaagca aaactgtgaa agatattatt tagcattatt
    41041 taagaaaaag caaaactgtg aaagatatta tttagcatta tttagtaaaa agcaaaactg
    41101 tagaagatat tatttagaat tattatagta aaaagcaaaa ctgtagaatg tattatttaa
    41161 gaaatagcta aaaatataaa atatactatt tgtaattata taaagaatat caataaatat
    41221 acacagtagt atttatataa tttacaagca aaaattatta aaaaaataaa aaacttattg
    41281 acaaaaatat tataaggaag tacaattgta ttaacaattt aaggaaacaa atccagtgat
    41341 agagaaagta gcatatatgg atttacagcg agttagggat tggtgtaagc ctagcaatga
    41401 agtttatgtg aagagagctc tggaggagat tatcagaaat tttagtatga taattggtct
    41461 gcccgcctta agggattgag aggataagta cttacttatc aaaaagagtg gtaacgcgga
    41521 tataattcgt ctcttagctg taaagctaag ggactttttt gatttaaaaa tcaaaatatc
    41581 catatagcta aagctaacaa tcacaaattt tgaaagtaat agaagtattt atcaataaga
    41641 gtggtaacgc ggtaaagtcg tctcttagtc attaaagact gagagacttt ttttgtaact
    41701 cgaaacattt aagtattaaa atttgaggga aattataatt aaaagagagg ggtagttaaa
    41761 atgaaagtat ttaaaaaatt attaagttta ggattagtat taggattgac attatcgcta
    41821 gtaggatgta gcggtggagg agaaaagaca aagttagaac aaataaaaga cagtggaaaa
    41881 ttggttgttg gtactagtgc agaatttcct ccatttgaat ttcacaaggt agtagatgga
    41941 aaagattcaa taaaaggatt cgatataatg ttggctgaag aatttgcaaa agaacttggt
    42001 gtaaaagttg aaattaagga tatgtcattt gatggattga taggtgcttt aaatgcagac
    42061 caagttgata ttgttcttgc aggtatgtct ccaacaccag agagagaaaa gagtgttgat
    42121 ttctcagaat tatattattt aagtagaaat gcagttatag ttaaagatgc agatatagac
    42181 aaagtaaaaa ctgaagacga tttaaagaaa cttagagttg gagttcaagc aggaagtatc
    42241 caagaagaat atgtagttaa cactttaaaa atgacaacta caaaatcttt aaaagcaata
    42301 cctgatttaa ttacagaatt aaaaaatgga aacatagatg cagttgttac taatgaagcc
    42361 gtttctttaa taaatgttaa aaaatatgat ggaataaaaa tggcaaatac tgaagttgga
    42421 aaaaatgtaa cagaaggtat ggctgcagca atcaaaaaag ctgacaataa caaagagttt
    42481 atagaactat taaacaaaaa gataaaagaa ttacaagatg gtaagaagat agaagaattt
    42541 ttaaatgaag catctactga agctgcttca aattagtaag aaagttatgt gggagatgaa
    42601 attatgagtt tagattttag ttttttaagt agatttggga cttctttttt ggaaggaaca
    42661 ggtgtgacag tatcaatttc tcttgtggca ttatgctttg gatttataat aggtataatt
    42721 atctgtatgg caaaaatatc aaagagcaaa gtattaagag caatatcatc aatctatatt
    42781 gaggttttaa gagggactcc attattagta caaatatata tagtatggtt tggtttacct
    42841 caattaggaa taagattccc tatgttgttt ggtataccaa gtgaatttat agctagtgca
    42901 tttgcattat cagtaaactc tggtgcatat gtggcagaga tacttagatc aggcatacaa
    42961 tcagttgata atggacaaat ggaagcaagt agatcattag gattaaatta ttggtcgact
    43021 atgagatata taataatacc tcaagctata aagaatatat taccttcatt agcaaatgag
    43081 tttattacac ttgtaaaaga atcttcaata atatctgtaa taggagttgt tgagataatg
    43141 cgtactgctg atatagtaaa aaatgcagca tttagagcat tagagccatt aatagtagca
    43201 gcagcaattt actttgttat aacatttact ctatcaagat tagttggatt attagaaaag
    43261 aaactatctg tgtctaacta gatgagagca tgtttagcat ttagggaaaa catagaatag
    43321 taaaaaggaa agtgataata tgattacaat aaaaaattta agtaaatctt ttggagattt
    43381 aaatgtatta aaaaacatag acttagaaat cgctaaaggc gaaataatgg ttatagtagg
    43441 cccaagtggt tctggtaaaa gtacacttct tagatgtatg aatttactag aaatacctac
    43501 aggtggagaa ataatatttg aaggaaaaaa tttggtcgat aaaaaaacta atattgatga
    43561 ggttagacaa aatataggta tggtgtttca gaactttaat ttattcccac ataagactat
    43621 tttagataat ataacattag caccaattaa attaaaaaag atgactaagg aagaagcaga
    43681 aaaaaaagca gaagttttgt tgagtagagt tggtctttta gataaaaaag attcatatcc
    43741 atcacaatta tctggaggac aaaagcaaag aatagcaata gctagagcac ttgctatgga
    43801 accagatatg atgttgtttg atgagccaac atctgctctt gacccagaaa tggtaaacga
    43861 agttttggat gttataaaag aacttgctaa agaaggtatg acaatggcaa ttgtaactca
    43921 tgagatggga tttgctaagg aagttgcaga tagagttatt tttatagatg gaggaagtat
    43981 acttgaagat aatactccag aagaagtttt cggaaatcca aagcatgaaa gaacaaaagc
    44041 atttttagca aaagtgttat aatatataaa aaggatgtta ttgacattag aaattcaata
    44101 catccttttt taatgatggt taatataaat tttaaaacta aagttatatt aatattaact
    44161 ttagtttttc aaatctttgc aaaaaaaaaa gagctcgggg taagctcttt ttttccaccg
    44221 aattaatttc cgttactaat ccgattttta ataaatataa aatatatcct tatgaaaggg
    44281 tattgggaat agatatatta actaactagg gttaccaggg ggataacctt aatttattat
    44341 aacaaaatgc gacacaaaaa tcaatatttt ttttggaata aattataaaa gttattttta
    44401 taacaagtaa gcttataaat ttaactttta tagtaaaaat aagtatatgt ataatataat
    44461 atatttattt tatataatat attattttat catatattgt attttataat gtatttttgt
    44521 agggtatagt ataagtatat tatattttac tataaaaatt tgtaataaag tgaagaaact
    44581 agcttttcat gaaataatat tttataattt aagattgtct ttttaataaa gattaacaaa
    44641 aaaaatacta taattaatgg tataatagtt agagttgaag attttaaata aaggtaggtg
    44701 taattaagtt ggaaatatac ttagacaata gcgcaactac aaaaccttat caagaagtta
    44761 tagataaaat ggtttatgca cttaatacgg aatatggaaa tccttcttct gtccatagaa
    44821 aaggtgtaga agttgaaaag ggtataaagg aagtaagaca agatgtagct aaatctctag
    44881 gagcgaaaga aaaggagatt tattttacat caggtggtac agagtgcaat aatactataa
    44941 taagaggtat cactagtcta aataaaaaaa gaaaaaatca cataatatct actaacatag
    45001 aacatccttc tgtgttaaat acattaaaag atttagaaga agatggtttt gaagtaactt
    45061 atttagaggt cggaaaagat ggaaaaataa atatagagga tttaaaaagt gcgataaagt
    45121 caactacatg cttagtaagt atgatgcatg ttaacaatga aatagggact atacagccaa
    45181 taggagaagt cggaaagtat ttgaaaggtt taaaagaaaa gatttatttc catgttgatg
    45241 ctattcagtc atatggaaaa attaacttta gaccatctaa atataatata gattttatga
    45301 gtgtaagtgc acataaattc catggcccaa aaggaattgg atttatgtat ataaaagaaa
    45361 ataatcgcct taagcctatg ttaacaggtg gtggtcagga aattggaatt aggtcgggaa
    45421 cagaaaatgt acctgggata tatggtcttg gagaagctgt tagaatactg aataaagatt
    45481 tagatgctgt catttcgaaa gtaaataact taaaaaatac attaaaaaat gaaataattg
    45541 ataatataga agatataaaa attaattctc ctgaagatgg agtatgtcat atattaaatg
    45601 tatcatttag agggacaaaa ggagaagttt tacttcatta cttagagcaa aaaggtattt
    45661 atgtatcaac tggctctgca tgctcttcta aaaagaaggg tagtcatgta ttaaatgcta
    45721 taggtctaac aaatgaagaa ataaatggaa caataagatt tagtttatct gatatgaaca
    45781 cagaagagga aatgttagaa gctgtgaaag ttttgaaaga atcaatttgt gacttaaggt
    45841 caataatgaa aagaaaataa agaatactgt ataaatagtg agaataaatt agtttaagag
    45901 gtgaaatggt gtataatata ttaatagtaa agtatggaga aattggagta aaaggaaaga
    45961 atagatatat atttgagaat aggcttataa gaaatataag aaatatgtta aagccaattg
    46021 gcaaatttaa tgtatataaa gagtatggaa gaatatatgt tgatttagaa gattatgatt
    46081 atgaagaagt aattgaagaa gttagaaaag tatttgggat agttggtgta tgtcctggag
    46141 taagagctaa aaaagattat gatacattaa aagaaatagc tttaaaaatg ttagaagaaa
    46201 aaatagatgc tggatataaa acttttaagg tagagtcaag aagaggagat aagtcttttg
    46261 ggttgacttc tcaagaaatg agtatggata taggtggata cttattatca aaggtaggag
    46321 ataagattaa tgttgatgta agaaatccag aagttaaaat aaaatgtgaa tacagagaat
    46381 ttcatactat ggtctatagt gatactatac ctggatatgg tggattgcca cttggaacga
    46441 atggtagagc tatgtctctt ttatcaggag gtatagatag tccagtagct acatggatgg
    46501 ttgcaaaaag aggtatggag gtagaggcag ttcattttca tagttatcca tttacaagtg
    46561 aaaggtcaca ggaaaaagtt aaggatttag ctaaaatact ggcaaaatac tgtggtagag
    46621 taagacttca taaagttaat atattagaaa tacaaaaagc aattggtgag aattgcaatg
    46681 aagaagaagc tactattctt tcaagaagat ttatgatgag aatagcacag agactttctg
    46741 aaaaaagaca ttgtgatgct ttaattacag gtgaaagcat tgggcaggtt gcatctcaaa
    46801 ctatacaagg tcttacttgt actaatgccg ttgtagattt accagtgttt agaccactta
    46861 tagccatgga taaatcagat atagttgata tagcaaaaaa aataggaact tttgaaacat
    46921 ctatagtgcc tgaagaagat tgttgtagtg tattttctcc tagaaagcct gttactaaac
    46981 ctagattaga gaaaatagaa aaatctgaaa cagcacttga tattgaaaaa ctagtacagg
    47041 atgcgattga caaaatagag gtagaggata ttgagtttta gtacaaaagt tatttaatta
    47101 ttaaattgaa tattgagcat attggtagtt ctgttgaaag gacttatata gacgatttat
    47161 taatatgtag tatttaaaaa taagtactat aattttattt aatgggaatg attattatga
    47221 ataaaaaggg ttttacacta attgaattgt tggtagttat atctataata ggaattttag
    47281 ttatagtagc tgttccagcg ttatttagaa atatagaaaa aagtaaggcg gttacatgcc
    47341 tttctaatag agaaaatata aagactcaaa ttgttattgc aatggctgag gaaccaagta
    47401 aagataagaa taaagtcata aaagatgtac tagaaaataa agatggtaag tactttgaaa
    47461 cagaaccaaa gtgtaaatca ggtggaatat attcagcaac gtttgatgat ggttatgatg
    47521 gaataactgg aggagaaagc attgcaaaag tgtatgttac ttgtacagaa catccagacg
    47581 gtgttgaaat ggctagggat gtacatcaaa gtatgaaaga tttgattgca tcatttgcac
    47641 aagacccttc tataatacca ggagcttcaa agagtaatga tgattttaga aaatatttat
    47701 tagacaataa atataaaaag ggatggccta caattccaga tgaatttaaa gcaaaatatg
    47761 gattaagtaa ggatacacta tatatacaac catatgcata taatcctact aaacctgatg
    47821 ccactgtagt tgtatttgca aataataaga ctggaggtaa ttggtacact tccctagttt
    47881 acgattatga tgaaggtaga tggtataaag gcaaaaatgg tatttctgtt gcaggtaggt
    47941 catgggatgt tgatacagat agtgttaagt ctgtaaaaac agagattcat tctaaagagg
    48001 gatggggtcc tttaaattaa tatataatgt tttattaatc tggtattgat taaactatag
    48061 aaaagagcag tttctataga tgacgaatac cagattttta tttatatgta taattaatat
    48121 atattaaaag tatttatatt tttaagtctt cacatgtaag ctaaaaataa ttataataat
    48181 gttgtaaaga agtttaacta caagattaat tttctgaaga tttagtattt atactaagaa
    48241 atacacaaaa aggggaatag atatgattaa taattttcaa gacaaccaaa caataaattt
    48301 aatacaaagt cgtagaagta tacgaaaatt tacaacagaa caaattagtg atgagcaagt
    48361 aaacacatta ttacactgtg catttgcagc accttcagga tgtaataaac agccttggca
    48421 cataactgtt gtgcaagacc agaaactttt aaaagcgatt agtgatgata ctttatctag
    48481 gattcatgag gtgagtaatg ttgagataaa taaaaacttt aagttatttt atggtgctcc
    48541 aactgtttta tttatttctt atgatgaaag tagctcatgg gctccatatg atgtaggaat
    48601 acttacaggg aatataacaa cagcagcaca agcactaggg cttggaagtt gtataatagg
    48661 tatgattaga ggattattta cacctgttga acaaggagat attgaaggtc ttgtgagtgt
    48721 attggataaa gaagatgtaa aagaatcaga gtcaataaag atgaagtttg atacaaataa
    48781 aaaatacaga gagatacttg atataccaga agggtattct gtaccatttg gtattgctat
    48841 aggtattcca gatgggaatt taccaaatgc tagagaggta gtttataaag tttctagagt
    48901 ttaggataaa gtattgcaac aatcaatcgg ttatttgaag aattaaatcg gtaatatcca
    48961 aataaactaa tagaagagaa agctatttaa aaagtagctt agatttttta ttgtaaaata
    49021 attaaataaa gaatagggtg ataagtattt tatttattat cctattcttt tttatttgta
    49081 gaatgataaa aaaatgataa aaacaagaat taagtgtaat acaatgtaaa taaagtgtgt
    49141 taattttgag tatatgttgt aaagtttatc aaaactgaaa aactcaagat atattttcag
    49201 tataatagtg tataaaaata gggtaaagtg gaagtctcga agtctcttaa aaatttgcac
    49261 ttttccctat tttattatgt tttctataaa ggttaatata cactaactat tatatacttt
    49321 cactccaaat ttagagatta tttcactagc tttttgaaca tcttccttag tggcatcagg
    49381 aacatctttt agttcataat cgatacctaa attttcccat ttgtgtattc ccatagaatg
    49441 gtatggtagc aattcaaatc tttctacatt tttcaatgta gctacaaatt tacctagttt
    49501 ttctaaatta tctacatcat cagtaatacc aggaactaaa acatgtctta tccatacagg
    49561 aatatttctt gattctaagt gtctagcaag ttttaatgcc ttatccatac caacaccagt
    49621 caaatcaata gatttttcct caaccatatg ttttaaatca agtaaaacca aatcagtatt
    49681 gtctaaaact gggtctatag tttctatatc tacgaatcca gatgtatcca agcaagtgtg
    49741 tatatcattt tcttttgctt ttttaaataa ttcacttaaa aactctggtt gaagagtgga
    49801 ttcaccacca gaggcagtta tacctccatc agaaaatttc ataaaagaag tatacttaag
    49861 agcatccgtt attatttcat caactgtata ttcttttcct gatttaatat cccatgtatc
    49921 cctgttatga cagtatttgc atctaagagg acacccctga aaaaataata tatatcttat
    49981 tcctggtcca tctacagtac caaacgtttc tatagaatgt atttttccct taatcataga
    50041 gttcacctca caatatattt taataacctt atcaatagtt ataatatttc tctaaaaaag
    50101 gccatcttat accagaaata attcaaatat aagatagcct tttattttta ttttctacac
    50161 agtagtaatc ttagttaaag tataaaaatt taaagactaa gccattttac cgtggaatgt
    50221 tctgtttata acatctaatt gttgctcttt agttaatttt atgaagttaa cagcatatcc
    50281 agatactctt atagttaatt gtggatattc ttctggatgt tccatagcat cttctaaagt
    50341 agctctatca aatacattta catttaggtg atgagcattt tgagcgaagt atccatccat
    50401 cattgcagaa agattgttta ttctttcagt catatctttt cctaaagcag caggtactat
    50461 agagaaagta ttagatatac catcttgagc gtgctcatat ggtaatttag caactgaaga
    50521 taatgaagct aaagcaccat tagtatctct accatgcatt gggttagcac caggagcaaa
    50581 tggagcacca gctcttctac catctggtgt attaccagtt ttcttaccat aaacaacatt
    50641 tgaagttatt gtaagtatag attgagttgg ataagaattt ctataagttt tattttttct
    50701 tattttattc ataaagcttt caactaactc tacagctata ctatcaactc tatcatcatt
    50761 gtttccgtat tttggataat ctccttctat ttcaaaatca acagctattc cttgttcatt
    50821 tctaatagtt ttaaccttag catgttttat agcagataat gagtctgcac aaactgataa
    50881 tccagccata ccacaagcca tagttctaaa tatatctcta tcatgtaatg ccatttctaa
    50941 tgcttcataa gaatatttat catgcatata atgtattaca tttaaagtat ttacatataa
    51001 tgttgctaac caatcagtga atggttcaaa tttactcata acttcatcat aatctaagta
    51061 ctcagaagtt ataggttcaa atcttggtcc aacttgaacg ccagatttct catctacacc
    51121 accatttata gcatataata aagttttagc caagttaact ctagctccaa agaactgcat
    51181 ttgtttacca attctcattg cagatacaca acaagctata ccataatcat ctccccagta
    51241 tgctctcatt aaatcatcat tttcatactg tactgaactt gtatctatag atactttaga
    51301 acagaaatct ttgaatcctt gaggtaattt agttgaccat agaactgtta agtttggttc
    51361 tggagaaggt cctaatgtat atagagtatt taacattcta aatgagtttt tagtaactaa
    51421 tgttctacca tctattccca tacctcctat acactcagtt acccaagttg ggtctccaga
    51481 gaataggtta ttgtaatcag gagttcttag gaacttaacc attcttaatt tcataacaaa
    51541 atggtccata tactcttgta attcttcttc tgttacgatt ccagctttta aatctctttc
    51601 aaagtatata tctaagaaag tagatgttct acctaaagac attgcagcac cattttggtc
    51661 tttaacagca cctaaataac caaagtataa ccattgtaca gcttcttttg agttagttgc
    51721 tggttttgat atatcaaatc cataagattc agccattttt ttaagttcat ttaaagctga
    51781 tatttgctca gttatttctt ctcttaatct gataacttcc tcatccatac aagaaacttc
    51841 taatgatttc ttttgctcat ttttatcttc tattaaagca tcaacaccgt atagagcaac
    51901 tcttctgtag tcaccaatta ttctacctct accataagca tctggaagac ctgttattat
    51961 accagatttt ctagcagctc tcatttctgg agtataaaca tcaaaaactc cttggttgtg
    52021 tgtttttctg tatttagtaa atatatcttt tatttctggg tctaattcat atccaaaagc
    52081 ttcgcatgaa ttttcaacca tcttgatacc accatatggc atgacagctc tttttaaagg
    52141 ggcatccgtt tgtaacccaa cgattgtttc taattcttta tctaaatatc ctggagcata
    52201 tgcagctatt ccagaaacag tttttgtatc aacatctaaa gtaccaccat tttctctttc
    52261 ttttttaaat agaaccattg ctttgtccca taatttttta gtattttcag tagcaccagc
    52321 taagaaagag tcatttccct catatggaga atagttcaat tgtataaact ctctaacatt
    52381 tatttcttta gtccatcttc ctgttttaaa tccttgccat gcattcatta agtatttcct
    52441 ccttagattt aaatgataaa taatactatt aaaattaatt tttatatact taatttattt
    52501 tatatatttt ttaaaaaata tattttctaa tataataata acatattatt cgtatcctgt
    52561 atacataaaa aataaaaaat tgtcgcttta aagtataaaa cagattatat gttaataata
    52621 tggtgaaatt atacccatat acaaataaaa taaaacaaat aaatagctaa ttaaggagaa
    52681 aatatttttt attttttgtt tagaataaat aaattttatt atggaaaaaa gaaaaagcat
    52741 acatcttcac aaactgaata tttatttaat agggggaggt gatatattgt ttatcacagt
    52801 ggtattattt ttagtaggtt tcctattaat tactaagggc gcagatatct ttataaattg
    52861 tacggtagag ataggtagaa aaacgaatat atcagaaatt atacttggag ctactatagt
    52921 tagttttgca acaacattac ctgaatttac agtgtctcta cttgcttcta ttgatggtca
    52981 tactactatg agtttaggaa atgcagttgg ttctataata tgtaatacag ggttagcatt
    53041 agggttggtt gtttttctaa gtccatttaa tgtagataaa aaaatgtttt tttctaagtc
    53101 tttactatta atagtatccg ttatagtttt aattttattg agtttagatg gagttataac
    53161 aagaggtgat tcgttattac ttataataat attaatcttt tatatggtta ataattatag
    53221 aagtgtcgtt ggaaaatctg atactaaaag tagaaatatt aaaaataaca ctattaaaga
    53281 catatctact aaaaaaggga aaaattatag aggtttttcc gtattagaaa ttgtaaaaat
    53341 actattactt tttgctacgg gattgataat gatgataata ggttctcaaa tccttataga
    53401 gagtggggta agaattgcta gctttttaaa tatacctcaa ggtatagtaa gtttgactat
    53461 aatagcactt ggaacttctc ttccagaaat agtttcatca attacagcta taagaaaaaa
    53521 tcatcatgaa atatcagtag gaaatatttt gggggcaaat atattgaata tagtgtcagt
    53581 aatagctata tcagcaatac caaataatat accgatacta tctcaaaata gacaattaga
    53641 tataccattc atgatattat tgatattaat agtaataata ccaacactta aatcaaatag
    53701 acttagtaga atacaaggta tattgatgtt attcacatat tttctgtaca tatcaatttt
    53761 gtatttcatg tatattattt aactatactg ttaaaaatat taattgcaga atattctaaa
    53821 aaagtatata atggataggt taagattaat tgataacgga gaggaataat atatgaatat
    53881 aacaaagttt gaagatttac caataagtga gggaataaaa aaagcaatag ctgaaatggg
    53941 gtttgaagaa ccatctccta tacaagcaca gtctatacct gcgattttgt caggtaaaga
    54001 cgttatagga caggctcaaa ctggtacggg aaaaacagct gcttttagta tacctatact
    54061 agaaacaata gacccaaata atagaagttt acaagctgta gtactttgtc caacaagaga
    54121 gcttgcaata caagtatcaa cagaaataag aaaactagct aaatattcgc acggaataaa
    54181 gacactacct atatatggtg gtcagccaat agatagacaa ataaagtctc taaagagtgg
    54241 tgtgcaagtt gtaataggta ctccaggacg tacaatagac catattaatc gtaaaacatt
    54301 aaaaatggat aacgtaaaaa tgattatatt agatgaagca gacgaaatgc tagatatggg
    54361 atttagagaa gatatagaaa tgattttaag taagattcca gaagaaagac aaacaacttt
    54421 cttttcagct actatgccaa gaggaatttt ggaattaact aagagatatc aaaaagaccc
    54481 agagcatata aaagttgtta gaaaagaact tacagtttca aatacaaagc aatactatat
    54541 agaaactagg tcatcaaata aattagaagt attatgtaga ttagttgatg tatatgaccc
    54601 taaattatct gttgttttct gtaacactaa gagaaaagca gatgaattag taggagactt
    54661 acaagcaaga ggatattttg cagatgcatt acatggtgat ttaaagcaga ctcaaagaga
    54721 tatagtaatg gataagttta gaaatggtac aatagatata cttgttgcta ctgatgttgc
    54781 agctagaggt atagatgttg atgatgttga atgtgtattc aattatgatt taccacaaga
    54841 tgaagagtat tatgttcata gaatcggtag aactggtcgt gctggtagag aaggtatgtc
    54901 atttactttt gtatttggaa aagaaatgag aaagatgaaa gacatagaaa gatacactaa
    54961 atctaaactg ataaagcata atataccaac gataactgat gttgaagaga aaaaagtagg
    55021 tactttcttt gcacaagtta aacagactat cgaagaaggg catttaacta aacagctaca
    55081 gtggttagaa aatttctgta atgatgaaga ttatgcaatg gttgatatag ctgctgcact
    55141 tgtaaaacta tctctaggtg aagaaatgaa agaagagata atagaagaaa aacctagaag
    55201 agagcgtgga gatagaaaag gtggtactgg agctaaagat ggtatgatta gattatttat
    55261 caatattggt agaaatcaaa gagtacaagc aaaagatata gttggtgcta tagctggaga
    55321 agttggaatc ccaggtaaag tagtaggcac tatagatata tatgataagt atacttttgt
    55381 tgaaatacct aaaaaagatg caaaaacagt gattgaaaag atgaaagata ttaagataaa
    55441 aggtaataag attaatatag aaaaagcaaa taaaaagaaa aaataacgta tagtatacaa
    55501 aactataatt agaaaatata taaaataata tattgaatat ttaaaaagcc tgatgtgttt
    55561 tcaaaacaga tagtagataa tctgtaatag ttaacagcat aaggctttta ttgttttata
    55621 taatattatt gttaggagtg tattaaaatt agtttataaa tagattcaat atacaagaaa
    55681 taatatatta aaaatatgta attaaataaa atttaatata tggtttttgt ttaaaagtag
    55741 aatttaatac tttaatttat aattttccat gctattgtga aaaagtgtat attagttctt
    55801 ctttttcaaa tagtatttga aaaagagaat attaattatc tatatagttt agattataat
    55861 aaaaaatgta agataacaaa ggggagaaaa agatgtcaat gacttatatg gaccctaggg
    55921 tttttttgat tttagaaatg gtgttggaag aagatacaaa tactttagaa caattttcaa
    55981 gtgttctagg tgtcagtact aggactatca gaaactatat aaaccagatt aatcgagaat
    56041 taaaagatgg tattgccaaa atagttaaaa atgaaaaagg tacatatagt ataagagttg
    56101 aagatgaaag taaattaatt gagatagtaa attttaatag agtaaagctg tcaaatttta
    56161 taaatttaaa ttcacctgat gaaagaataa attatgttct tgatatttta actatgacta
    56221 acaatccaat tactatagat gatttggctg aagagatatg tataggaaga acaacattaa
    56281 taaaggattt aaaaaaagca gacagaattt tagctgaata cgatttagaa ttaaaaagga
    56341 aaccaaatat aggtatgatt ttagttggtg aggaaatgga tataaggttg ctagtccttg
    56401 acagattgta tgaaaattat attgatgttt tggaaaacgt taatcaaata aatttagtta
    56461 aagattatga tattgaatgt cttagagatg aattaaaaaa gttatttaaa aaagaagaac
    56521 tatacataac agaacaggta ttgagagatg ttgaaagata tataataata tctgctttaa
    56581 gaaatctaaa tggttataaa tttgaaaaga tagataagag atttgaagta ataagatgtt
    56641 cagatgaata cactttagga ttaaaactca aagctttatt ggaaaagata ttcaatatag
    56701 ttttaaatga aaaagaaacc atatttttga caatgccatt aataggtaga aaagctccta
    56761 gtacaaagtt agcacttagc agtataaaga taaatgatag tgtaaaggaa gttgtagatg
    56821 aagtaactag ttttttatta gagacctcag gaattgattt taaagaagat aaaaagctta
    56881 tagaaaattt agaatatcac ttgtatttcg cacttaatag aatgagattc aacatacgag
    56941 taaaaaatcc acttttacaa gaaataaagg aaagatatac atttccatat agtgttgcaa
    57001 agatagctgc aatgataatt gaggaaaagt ttgacttgaa aatatgtgac cacgaaatag
    57061 gatatatagc attgcatttt ggaagttatt ttgaaaaaaa ttcaagaaaa gttctaaatg
    57121 taaatagggt agctgttgtt tgtggaacag gtcttgggac tgcacaactc attagaataa
    57181 agataggaaa aatacttgga gagagtgtag aagtaaatac tttttctgat atagaagcta
    57241 aagctaattt agatttatta aagtactatg atttagtatt tacaacagta gatttcaaga
    57301 cagacttaga tgttccaatt tttagagtaa atgcgttatt taatgaagaa agtataaaga
    57361 aagagataga agatgcaata cttttgaaaa atagtataaa tattaagtct ataaaaaaga
    57421 ttgaaaagaa tattcctttt ataggtaaat taattgaaga ggataagttc tttatattga
    57481 ataagggtac attcatggaa aatttagaag agatgttaga gaagctagtt cttaaaggaa
    57541 ttattgatat agagtttaaa gaaaatgtac taaaaagaga aaagaaatct tcaacagcat
    57601 tagataatta tattgcattg cctcactcag ttagtcctaa tgaagagaac ctttatttgg
    57661 catttggaat attagaaaat ccagtaattt gggatacaaa ggaaattaga ataataatat
    57721 tgatgataat tccggacaag aatgtgggta gtacagaact gataataaaa agctatgaag
    57781 aagtacttga aataggaaga gataaaaaaa tagtagaaaa actttctaaa gtaaaatcat
    57841 ttgatgaatt ttacaaaata attaccacga ggaggtaata atatggaccc agtaattttt
    57901 attatagtat catttattgt agtttattta ttgaatattt tccttggata tttacagatt
    57961 aaagatttta ataagaatta tattgaacta aaaagaaaag gaagagtggc aataggcaga
    58021 aagaaaggaa gaatttcatc aggtgcaatt gtgttgatac ttataaatga tgatggtgtg
    58081 attatagaga ctagaaaaat gcagggtgtt acagtagtag caagagtaaa gtcatttgaa
    58141 ggtttggtag gaaaaaactt aaagtacata gaaaaatcag atttaactgt ttacaataaa
    58201 ctacttaaaa aagcaatact agatgcagtc aaacagtaca taacatttga gaagaataaa
    58261 tgtgaagata tgagtaatct tagtactgat gaagaatgta ggatggaagt agtttaaaaa
    58321 aatatatcta taaggaggag ttaatatgac acaatttttt caagtattag cgagtggtgc
    58381 agatagtttt atgaatttat ttagggcagg tggacaacaa tttgtaggtt ttgtaacaga
    58441 tatagtacca cttttagtag cattattagt tacaatgaat gcaattatca actttattgg
    58501 tacagataga gtcgagaggc ttgcaaaaaa atgttctgca aatatattta ctagatattt
    58561 gatactacca gttcttggta catttgtatt tgcaaatcca atgacactat cattgggaag
    58621 attcttacca gaaaagttta aacctagtta ctttgcagca gcaagttttt catgccatac
    58681 aatgaatgga ttatttccac atattaatcc aggtgaacta tttatctatc taggaattgc
    58741 aaatggtata acaactttgg gattctcaac agcagattta gcagttagat atttgttagt
    58801 gggtatagtt gcaaatttta ttaaaggtgt agttactgac tatacaacaa aatttgtaga
    58861 gaagcaacaa ggaatagcat taaattcaga tataaagatt gggtaattac tttaaggtat
    58921 aagtaaacta cttaaataaa aagatagtgt atagaggagg agaaattata tgtcatttaa
    58981 caaagttaaa ataataaagg gttctggagg atggggtgga ccattgataa tagaaccaac
    59041 agaaaagaag aataaggtag tatatgtgac tgggggagca aagccagaaa ctgcagtaaa
    59101 aatagctgaa cttacaggat gtgagttaat agatggattt tcacaaggtg ttaaagatga
    59161 tgaaatcgca tgtgtaataa taaattgtgg tggaacattg agatgtggta tatatcctca
    59221 aaagaaaata ccgactataa atataatgaa aacaggaaaa agtggaccat tagcaatgtt
    59281 tataaaagaa gacatatatg tttcaggagt aaaaccaaca aatgtagagc agttataatt
    59341 tagatagtag gaggaaagaa atatggctta tgatactaaa aagaagataa gtgaacagag
    59401 tcaaggaatt gttgcaaaaa taggtatagc actaggtaac gtgacaagca ctctttttca
    59461 agctggtagg gatactatag atacattaat acatactata ctgcctttta tggctttcgt
    59521 tgcagtcctt ataggcataa taaatcaatc aggtatagga aattgggtag ccaatttact
    59581 tactccatta atcggaaatg ttggaggttt agttgtttta tcaataatat gttcatttcc
    59641 gttgttgtct ccatttcttg gaccaggagc tgttatagca cagatagtgg gtgtattggt
    59701 tggtacagaa ataggaaaag gaaatattcc gccaagttta gcattaccag cattgtttgc
    59761 aataaactca caagcagcct gtgactttat accagtaggt cttggtcttg cagaagctga
    59821 acctgataca gttcaagtag gagttccatc agttttatat tctagatttt taacaggtgc
    59881 gccaaccgtt ctattagcat ggttagcaag ctttgggtta tataaataaa aggatacttt
    59941 ataaataatt agagagaggt tcaattgata tggaattaat atacagtact caaataaata
    60001 aaataggaga taatgcaaat gaatttttac aacataatat gtttgtaact ttcaaagata
    60061 atgctcctca agaattagaa aattactgtt atatacattc tgaaaataat ttagtaaaag
    60121 aaatacttac gaatgatgta ttatcaataa ataatgttga ttataaaatt acttctgttg
    60181 gtgagttagt aaaccaaaat ctttcagaat taggtcatat aacatttaag tttacaggtg
    60241 aaaaagatgg agcaattgga gggaccttat atcttgaaga aaaagaaata gcacctattg
    60301 gcgtaggtac tatactaaaa ataattagaa aataaaattt aaaacgaatc taatcatatt
    60361 ataagtaaat tatagataaa agtcaattta actaatcaag ttaactttta atatgataca
    60421 aaggggatat gaaaatgaga aaagtagctt tagtcgttgg aggaggaaga agtttaggtg
    60481 aatatttatc taaacattta gctaaagtag gttatgatgt tgctattgca gatttaaatg
    60541 aagataatgc aaaaaaagta gctaaagaaa tagaaatgga atttggttgt aaatctgtag
    60601 gatatggatg taatgctata gatgagatac aggttatgag tactatcaaa gctattgaaa
    60661 atgattttga tagaatagat ttattagtgt acaatgcagg agtggctgta agtaaaaaaa
    60721 taacagattt tggatacaaa gattttaaat ttattgtgga tgtaaattta aatgggtatt
    60781 ttctatttgc aagagaagtg tctaaagtta tgatagcaaa aggtattgag ggtagaataa
    60841 tacagataaa ttcaaaatca ggtagagtag gaagcaaaca taattcaggt tattcagcag
    60901 gtaaatttgc tggagtagga cttactcaaa gtttagcact agatttggca gaacataaga
    60961 taactgtaaa tgcactgatg cttggtaatt tgcttgattc tgacatgttt gaaagtttaa
    61021 ttcctcaata tgcagtaaaa ttgggaatac ctgaatcaga agttaagcaa gtttatatag
    61081 ataaagtacc tttaaaaaga gggtgtagat ttatggatgt tgcaaatata cttacatttt
    61141 atgcttcaaa ggaagctgaa tactgtactg gtcaatctat aaatattact ggtggacaag
    61201 ttatgtaaaa tttttatggg agtgtctcaa gatgaacttt ttagtttatg agacactttt
    61261 tttgtataaa attaaatata tttttagcat ttgaataaaa aagaggctta tctatatttt
    61321 gaatcaacct catttttgta attaagaaat atggtattta agtaagcata taagattttt
    61381 agttttttag aagagaaatt ttcactaata ttatatttat gaagtatttt taaacttaat
    61441 ttgataaaat atatctatgt tttgtatact gtagttttga acatataagg tttaataaat
    61501 tatagtatat atgttaaatt ttaataatgt aagaaaatag aaagaaggat attttatgga
    61561 tataacaaaa aaattaagtc atggatgtac actagattgc catgattgtt gtaaattcaa
    61621 tgtctatact aaaggaaata atgtagtaaa aattgaagga gataaaaatc atccttacac
    61681 aaaaggtttt atatgcaaaa aagggatggc tcatttagat agattaaatc ataaagatag
    61741 aataaagact cctatgctta aagttgatga agtatggaaa gagatttctt ttgataaggc
    61801 tatagaaata atggcagaaa aacttacata ctataaagaa aagtatacat caaagtctgt
    61861 catgcattat gaccaatatg gaagtgggtc tgtactaaaa tacataggag atatattttt
    61921 taatttttat ggaggtgtca gtaggcataa gggaagtcca tgttggagtg cagggatgca
    61981 tgctcaaaaa tatgattttg gagtagctaa gagccatgcg atagaagaca tgttaaacag
    62041 taaaagtata tttgtgtggg gaaaaaatcc agcatacact acaattcata ctatgcaaat
    62101 aattaaaaaa gctaaagaaa aaggtataaa gatagtagtg attgacccga tatatacgaa
    62161 aacagctcaa atagcagata agtatataca ggtaaatcct ggaacagatg gagctttagc
    62221 tatagctatg gcaaaaatta tagtagaaga taaattatat gatgaagaat atataaactc
    62281 atacgttatt ggttttgaag agtataaaag atatttatat tcgttggaat taagtttttt
    62341 gattgatgaa tgtggtgtta aagagaatga tataagagag ttagttgctt tatatactaa
    62401 caaatattcg agtataaatg ttggatatgg gcttcaaaaa tataaaaatg gtggaaacac
    62461 aataagagct atagatgctt taggagctat aactggtcaa ataggattta gtggtggagg
    62521 ggtaaactat gctaataaag tttacccaag tgttataaat tctgaccctt acaatagtaa
    62581 attatatggt caagataggg agttttatgt aagtaatatc agtaaattca tagaagaatc
    62641 tttaagaaat acatcaaata aaataaataa tgtatcagat gaacctgata caaaatcaaa
    62701 taaaacagat tatatatcaa atgacttata tagcttatct ggtaagtcaa ttaaagataa
    62761 tattcctata aagatggctg tgataactaa aagcaatatg ctcaatcaac tgccagattt
    62821 agtagaattg gagagggtgt tttcaaaaat tgaatttaaa gtatgttttg atatgtttat
    62881 gactgataca gccactctat gtgacctgtt tataccttgt acaaatacac tagagagtga
    62941 agatataatt tatagttcaa tgactaatcc atatataacc tataatgaaa gagctgtaaa
    63001 gccagcacat aaattgatgg atgaatatta cttctttatg gagctagcca aaaagatgag
    63061 attaaatgat tatccatttg tagaaaagag cacttattta gaaaaagtga ttgaaccact
    63121 taagtgtgtt gacaaaaatc tggatataga aaaacttaaa aacaattact ttactataca
    63181 taaccctgtt gcttgggaag ataagaagtt tgagactcct tctggaaaat acgaacttta
    63241 ttcagaaagt ataaaaaact tgggaataaa tccaacacct gtatatataa acaataaata
    63301 taaagagata gaagataaaa atgaaaatat ttcttttaga ttattaacta accatcatgc
    63361 ggatacttta tttagtcagc attttatgga taaaaaatct atagctcaag catatataaa
    63421 tcaaagaatg gctaaaaaag taggtatcaa ggataaagat actgtgatat tgaggtcaaa
    63481 aaaagcaaaa ataaatgtac aaataaatat agatgatgga gtaggtaatt atatagtaaa
    63541 aatgtatgtt ggatggtgga agaaacatgg aaatcctaat attttgacag acactggtat
    63601 atcagatttt ggtggtcagg taacatataa tgaaagtata gtagaaataa tacgacaaaa
    63661 ctagttaaat ttcattgaag gaataaaaat ataattatag aattgattaa aagttactat
    63721 ttttacaaaa ctattgaagg agggattttg atggtaaatt attccttaga acataagaat
    63781 ctatacatag aatttatgtg ctcagaacta gaccaccatg tagccaatga aataagggaa
    63841 gaaatagata acttattaag tgtaaatcaa gtaaaaaatg ttgtatttaa ttttgaaaat
    63901 attaatttta tggattcttc aggtataggt gtcatcatag gtagatataa aaagatttct
    63961 aatgaagggg gacgagtttc agtaattaat ataagttcac gagttaagaa gatttttgat
    64021 ttatctgggt taaataaaat tattggcatt tatgatacgt atgaagaagc tctaagttct
    64081 ttgtagggag gagtatgtga tgaataatat tatggaagtt aaattttcgg caatatctga
    64141 aaatgaaagt ttagcaagag ttatagtagc atcctttgca gctaagttag acccaacagt
    64201 agatgagtta gtggatataa aaactgctgt ctctgaggct gttacaaatg ctataataca
    64261 tggatatgaa gaagacagtt ctaagtttgt tttcttaaga tgtgagatag agggaaatac
    64321 tataaagatt atagttgaag atgaagggta tggaatagaa aatgtggaga aggcaatgga
    64381 accactttat acatcaaaac cagaacttga tagatcagga atgggattca cagtaatgaa
    64441 aagctttatg gacgatgtag aggtaagttc tgtaaaagat aatgggacta gaattgaaat
    64501 gactaaaaaa ataaatgtgc ctaaataaag tccaaaagtg aggttttatt atggaagtaa
    64561 ctgttgccag agaagaaaaa aaacctcttc ttagtcatga agagactcta gaacttattg
    64621 aaaaagttca aaatggagat gaagaggcaa aagaaatatt aatatcaagc aatttaggtc
    64681 tagttaggag cgttgtatct agatttttaa atataggata tgatagagaa gatttatttc
    64741 agttaggttc tataggctta ataaaatcaa tatataaatt tgacccaaaa tttaatgtaa
    64801 aattctctac ttatgcagta ccaatgattt taggtgagat aaagagatac ttaagagatg
    64861 atggtatgat aaaagtgtca aggtcgttaa agcaaatagc tgtaaaggca aagatggaaa
    64921 gtgaagcttt aactaaaaaa ttgggtagag aaccgagcat agaagaatta gctaaagcaa
    64981 tagatgtaga aaaagaagat ttagttatgg ctatggaagc taatttcaat gttgagtatt
    65041 tacaaggagt tattcatgaa gaggaaggct ctccaatatg tcttattgat aaaataagca
    65101 tgaagggaga aagtgaagaa gagaaagtag tggacaacat attgttaaaa gacatacttg
    65161 gaagattgga taagagagaa cgtcaaataa tagttctcag atattttgaa gatatgactc
    65221 aaagtgaaat aggagagatg cttaatatat cacaagttca agtatctagg atagagaaga
    65281 aagtattgtc taagttaaaa gaatacatat catgacaatc cttatgatat gtagaataac
    65341 aaaatataat aatataataa atgtattact aaaataaaat gtaataattt tttatgttaa
    65401 gtttttaaat tattaaaata tattaaaata ataaagagta taaaaaaata aaaatattaa
    65461 aacaaggtgt ctttatggga aaatagattt tttctttatt aggcatcttt tttatttttt
    65521 gactaaactt tagatagtca aaataaataa taggtttatt tttttaaaat taacaaataa
    65581 tactttagag aggtgtttag tatggataaa aattataaaa aatatgtaga ccaaataagc
    65641 ccaaaaccta catatttaaa aaactataca cttgcattta ttgtaggtgg aattatttgt
    65701 atgatagggc aagcaataaa tgatttgtac atgaaagttg gaggtcttga taaattaggt
    65761 gccagttctg caacatcaat aacattaata tttatagggg catttttaac tggattagga
    65821 gtctacgacc taataggaaa aagggctgga gctggttcta taattccaat aacaggtttt
    65881 gctaactcaa tagtatctcc agcaatggag tataaaagag aaggctatgt attaggtgta
    65941 ggtgcaaatc tatttaaaat tgcaggacca gttttagttt atggtatagg ttcttctatt
    66001 ttatgtggaa ttatttatta tatttttaag atgttttaga tgatttttat ttaatttaaa
    66061 ttttattaat tatgaagaat gtataaaatc tgaaaatagt tactaaagta gctagttgag
    66121 agataatcat gcatatttaa ttaatattaa aaaattaaat agtaaaatgc aaaattaaaa
    66181 aggtgtgaaa atatgaaaaa taaaagaatt ggaaaaagaa cagtcaagtt agaaaataaa
    66241 ccaacaataa tatcaactgg aactatagta ggtcctaagg aaggtgaagg accactaaaa
    66301 gactattttg atatgattat gaccgacgat ttatatggtg agaagacttg ggaattagct
    66361 gaaagtaaaa tggtagagac tgcatctcaa caagctatac aaaaggcagg taaaaaattg
    66421 acagatgtaa attatatgct aggtggagat ttaataaatc aaatagtgcc agcatcattt
    66481 gcagcaagag aactggccat tccattttta ggtatatatg gagcttgttc tacaatggca
    66541 gaaggtcttt gtataggttc tatgcttata gatggtggtt ttgcagactt agtactgtca
    66601 gggacatcaa gtcattattg tacagcagaa agacagttta gattcccgct agaacttgga
    66661 aatcaaaaac caatgacagc acagtggaca gttacaggag caggaagtgt attactagct
    66721 cctaatggag aaggaccaaa ggtaaaatat gttacagttg gtaaagtaat agatgagggc
    66781 attgatgatg gcaataatat gggaccagct atggctccag cagcgataga tactatctac
    66841 tcttattttg aagatactaa tgatgaccca aatagctttg atataattgc tactggagat
    66901 ttaggtactc ttggaaaaca gatagcagaa gattttttaa aagaaaaagg tatagatata
    66961 tcgaaagttt atactgactg tggtatagag atgtttaatc ttaaagagca agatgtccat
    67021 tgtggtggaa gtggatgtgg atgttcagct acagtatttg caggatatat atatgataag
    67081 ttgagaaaaa aagaatttaa caaagtaatg ctcgtatcaa caggagcact actttcacca
    67141 acaagtacac tacaaaagca gacaatacca tgtgttgctc atgcagtagt aatagtaaat
    67201 gagtaggaag tgatatatat gttaatggac tatgtaagag tatttattgt aggtggaatt
    67261 atatgtttaa tagcccaagt aatgatggat tattttaaaa tgcaaactcc atatgttatg
    67321 gtaacgtatg taacaactgg tgtagtttta acttttttag ggctttatga gccattagtt
    67381 aagtttgcag cagcaggtgc aacagttcct ataataggat ttggatattc acttgctaca
    67441 ggagtaataa aatcaataca gagtgatggt tttttaggca tttttctagg aggtacaact
    67501 gctacggctg gtggaatagc agcagccata ttctttggat atattatgtc agtcgtattt
    67561 actccaaaag caaaaccata aatctttatt taagagaata ataactttta gaaataataa
    67621 ctttttataa agtaggtgga tatcttatag attccaccta ttttttatat aaagaagcgt
    67681 ttacataatt gaagttgaca attatttaca acatagtgta gtattatatt atcaaaataa
    67741 aatgaagtga agtaggggat aaagatggta actattaaag atattgcaag gaatctaggt
    67801 atcagttatt caaccgtttc taggtgttta aacaataatc ctaatgtatc tgaaaaaaca
    67861 aaaaagaaag tagtagaaga ggcaaataga ctagggttcc attttaatgt aaatgcaaga
    67921 aatctggcaa aaaaagaaac caacagaata ggggtaatct tttcaaataa ttttaatcat
    67981 caagatacta gaaaattttt tagtgatata atggacagct ctataaattc tattgagact
    68041 aataaatacg attttataat acaaccgaat aataacatta gtggtgatag taatgtatat
    68101 aaaatggtaa atggacaaat ggttgacgga ttagtaattg tgtctcaatc aataaaaaaa
    68161 gaagagtatg attttttaaa agacaataat tttccacatg tatttatata ttttaaacca
    68221 tcttttatag gagaagtaga caatttcttt tgggacgata acgtatatgg aggttatata
    68281 gctaccaaac atttaattga ccatggtcat aaagatataa taacagtaac ttcagatgat
    68341 aagagttcaa aaatgcatga agatagaacc aagggatatt tgaatgctat ggaagaggca
    68401 aatttaaaga ctgaagtcat taaatctaaa atggattttg aatctcaagt tgaattttta
    68461 gaaaaatata ttgataaaat aaaaaaagct tcagctatgt ttgtacaaca agatgtacca
    68521 gcgatttcta taatacaaga attaaaaact acttatggta taaatgtacc agaagatatt
    68581 tctataatag ggtataataa tattgaatta atatcatact tcaggtcgca tttatcaaca
    68641 atagatgacc caagagagca agttataaaa aatggagtag acagtttagt aaatataata
    68701 aataaaaaaa gcacagagca tattccaaga aaactatatc caaggttaat aatccgaaat
    68761 agtgttaaaa gaataaaata gtaagttttt aagcattaaa attttataaa aaatattata
    68821 gattatgtga agtgttttta caaataatat aaataaaatt aaaaggagta ggtgtatata
    68881 agtacattgt actcctttat ttattgagag aatttttata atatgaatga ataattttat
    68941 tcaattattt gaaaatctat tattttaata atgcaaatta ataaattgtt tgcattttat
    69001 atcatgaatg gataatattt ttttgat
//
LOCUS       DAMJVM010000010        67742 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.010, whole genome shotgun sequence.
ACCESSION   DAMJVM010000010 DAMJVM010000000
VERSION     DAMJVM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 67742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67742
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.010"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            73..1185
                     /locus_tag="PC523_000724"
     CDS             73..1185
                     /locus_tag="PC523_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HDF2474281.1"
                     /translation="MNSRVKSIDIIRGLSIALMIVCNNPGTWMRMYPQLRHAVWHGVT
                     LADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNP
                     DLNTVRILGVLQRMGLVYFVTSLVYLLLKKLNVRSSVTIITFLCISAFIIVGYYIVAK
                     PYGFELEGSLAQLVDLHFFKGHLYKPDFEPDGFLTSIVAISSGMLGCMMGCVLLKENI
                     GEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGILLSIFYFICD
                     IKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNLPKLTNKVDYPTTLVEWTTYELIT
                     PWAGTTWDSLVFSLLYVLFWVIVMSIMYKKKIFIKI"
     gene            1440..3464
                     /locus_tag="PC523_000725"
     CDS             1440..3464
                     /locus_tag="PC523_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2474282.1"
                     /translation="MKKTTKLLATGMVSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGPTRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNITRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQN
                     TYDWLSSQGLEEAYYIGGSQSLSSKIIDKISKIAKNGTSKNRISGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAET
                     VYQIGDGMKDSVISSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIFYKVKDKNGGTTKTAASNILKRILEKFNMQNRGIKTRTLANGNDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTTEKVKTMGTQIGIGIEDTVK"
     gene            3684..4370
                     /locus_tag="PC523_000726"
     CDS             3684..4370
                     /locus_tag="PC523_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDF2474283.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4465..5520
                     /locus_tag="PC523_000727"
     CDS             4465..5520
                     /locus_tag="PC523_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HDF2474284.1"
                     /translation="MIKKISTILSLVLLISISSTMWVFADAKPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEDSIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTAKLDFGSRSDVNEYISLGVV
                     SAKENMPILICNDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5636..7192
                     /gene="murJ"
                     /locus_tag="PC523_000728"
     CDS             5636..7192
                     /gene="murJ"
                     /locus_tag="PC523_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDF2474285.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVVTLLIYNNLYAFIGSGTIKEIISVGAGVLGGASVYTILIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            7238..8944
                     /locus_tag="PC523_000729"
     CDS             7238..8944
                     /locus_tag="PC523_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDF2474286.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKDLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIIKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDVANDIIAEVNKIDDYSVIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRRVLSECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKSNGTDLIIGTDPDCDRVGIVVKDSSGEYIVLNGNQVGSL
                     LVRYIVESLVKENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEKSNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALLD
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKEGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8966..10039
                     /locus_tag="PC523_000730"
     CDS             8966..10039
                     /locus_tag="PC523_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HDF2474287.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDRNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKEEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEEISKYLGTKEEVEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10051..10773
                     /locus_tag="PC523_000731"
     CDS             10051..10773
                     /locus_tag="PC523_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2474288.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIRVLYEQGGVYMDTDMQILKDITPLLEDNR
                     LICGYEDDGEYMNAAILGVEKGHPFFKDLLEYYEKEILTSSLFTIPKIMTHLMEKNYK
                     KIDQNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTYGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10789..11946
                     /locus_tag="PC523_000732"
     CDS             10789..11946
                     /locus_tag="PC523_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HDF2474289.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTVFDKSTLLNRLLASRPKFWNVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11949..12788
                     /locus_tag="PC523_000733"
     CDS             11949..12788
                     /locus_tag="PC523_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2474290.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDANKIDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGDDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFVKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12803..13978
                     /locus_tag="PC523_000734"
     CDS             12803..13978
                     /locus_tag="PC523_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDF2474291.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHRFKGRNIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            14007..14756
                     /locus_tag="PC523_000735"
     CDS             14007..14756
                     /locus_tag="PC523_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2474292.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14778..15686
                     /locus_tag="PC523_000736"
     CDS             14778..15686
                     /locus_tag="PC523_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2474293.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALKGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15734..16486
                     /locus_tag="PC523_000737"
     CDS             15734..16486
                     /locus_tag="PC523_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2474294.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENIGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16464..17807
                     /locus_tag="PC523_000738"
     CDS             16464..17807
                     /locus_tag="PC523_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HDF2474295.1"
                     /translation="MPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLAEVGNENVV
                     CVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDVDVILIAVG
                     TPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYINRNLKNDVK
                     VELASNPEFLSQGTAVKDTLHASRIVIGVESEWARDILQQIYEPFNQPIIVTNRNSAE
                     MIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLKAGIGYGGS
                     CFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQDMKVAVLGL
                     TFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYADSVDDAIE
                     EAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKVKDMEELGIEYYSIGR"
     gene            17813..18904
                     /locus_tag="PC523_000739"
     CDS             17813..18904
                     /locus_tag="PC523_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2474296.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSVKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLTKELGIEDDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLSMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18987..20900
                     /locus_tag="PC523_000740"
     CDS             18987..20900
                     /locus_tag="PC523_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2474297.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNVFTTNDIIISACIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFMATLAYLI
                     PVYIINKLLGFDYPIIFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKISNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSNIKEVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVDIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            21088..21795
                     /locus_tag="PC523_000741"
     CDS             21088..21795
                     /locus_tag="PC523_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2474298.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAELTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKTVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYRWGATGPNSFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGNSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            22096..24207
                     /locus_tag="PC523_000742"
     CDS             22096..24207
                     /locus_tag="PC523_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HDF2474299.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANNPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGNGQN
                     KDNWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVSWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSSSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWPTNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNSSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKNNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTNL
                     VYTAGDGINQNTLNAFLNLVN"
     gene            complement(24405..25370)
                     /locus_tag="PC523_000743"
     CDS             complement(24405..25370)
                     /locus_tag="PC523_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDF2474300.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTIEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNAGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(25655..26611)
                     /locus_tag="PC523_000744"
     CDS             complement(25655..26611)
                     /locus_tag="PC523_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDF2474301.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPSLVNTILPYVKTNMNPNEIISLGKELLSIGNLDLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            26980..27768
                     /locus_tag="PC523_000745"
     CDS             26980..27768
                     /locus_tag="PC523_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2474302.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEAIKDDMMFISENGTLVVYKGKEILSNPLDKELVNEIIETTRSIEGKKI
                     VMSGKKYAYIESKDEAFIDEVSTYYAKFKVVDDLTKVEGDILKIAIFDFKGAEHNNSI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEYLKEIARFRAKRNTENGVVDKIKEVMKIG"
     gene            complement(27899..28369)
                     /locus_tag="PC523_000746"
     CDS             complement(27899..28369)
                     /locus_tag="PC523_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HDF2474303.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEYIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENAPQIENKILSIIKK"
     gene            28508..29152
                     /gene="uppS"
                     /locus_tag="PC523_000747"
     CDS             28508..29152
                     /gene="uppS"
                     /locus_tag="PC523_000747"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HDF2474304.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            29195..30100
                     /locus_tag="PC523_000748"
     CDS             29195..30100
                     /locus_tag="PC523_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2474305.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKVTQGVVALVKSMDKGNKTSQQQNVNSE
                     QFDLGNEEENKNKKYTVFIDPGHGGNDKGTESKTSHRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSDEQKIIN
                     EKYQEQLAEGIVQGVLSYLDSKGNK"
     gene            30412..31959
                     /locus_tag="PC523_000749"
     CDS             30412..31959
                     /locus_tag="PC523_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2474306.1"
                     /translation="MNKNSMTKIYSLNKIIFIIIGILGIVLLTRHIGVGVKVLILILL
                     ILFSINVFISHLKIQVYEDKINLGLINNPDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKYYFGKEAIYVKLKSAVFVLIIKLED
                     REQKIPYLVNLIKNKIVILNQIDNFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     IKSKRLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMIAPNKFIPIAEKNGLINNIGRWLIQEVFGTINKWILEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDVIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMNQVKVLSELGCNFIQGYVFFKPLDEINYKRLLTDGSYINLEDTLLDK
                     CMAVEEE"
     gene            31962..32360
                     /locus_tag="PC523_000750"
     CDS             31962..32360
                     /locus_tag="PC523_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDF2474307.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDNPIDAKMILKNEEIPTLTLYRL
                     LRKLCANNILTFNKEDGKKYMLNVSLDTISLRTIVEAIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            32400..34133
                     /locus_tag="PC523_000751"
     CDS             32400..34133
                     /locus_tag="PC523_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDF2474308.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     SHILNYCIYGADIDEKAISILKDSLINKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKKTYID
                     VFKIKNEDICINKFEILEELLKSSKFEHFNINQRLLSDEWILVNKEDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYVVDKSEY
                     RLIYSNDIDNENTNKKILDEIIGIYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLGIFSYEYLVGILNSSIYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYDEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            34365..35576
                     /locus_tag="PC523_000752"
     CDS             34365..35576
                     /locus_tag="PC523_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2474309.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDVSLRTVMESIIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRKLASNFEIRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            35768..36028
                     /locus_tag="PC523_000753"
     CDS             35768..36028
                     /locus_tag="PC523_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDF2474310.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            36381..38093
                     /gene="ptsP"
                     /locus_tag="PC523_000754"
     CDS             36381..38093
                     /gene="ptsP"
                     /locus_tag="PC523_000754"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDF2474311.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLEN
                     AVAVSKDELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTENVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDKETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEVQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLNMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADEVLNMGTAEEIKSYIEK
                     TFNI"
     gene            complement(38885..39550)
                     /locus_tag="PC523_000755"
     CDS             complement(38885..39550)
                     /locus_tag="PC523_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474312.1"
                     /translation="MKGKIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKIDNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NGNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDNDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAV
                     NLN"
     gene            39895..41325
                     /locus_tag="PC523_000756"
     CDS             39895..41325
                     /locus_tag="PC523_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2474313.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDSKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKALKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEEICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            41693..42271
                     /locus_tag="PC523_000757"
     CDS             41693..42271
                     /locus_tag="PC523_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDF2474314.1"
                     /translation="MFKRYAEKMTSVLICNNVIDNNESKVYSYGFEILIAFVVNIATM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENLNI
                     DLFKNIIMIIASVSWVGICALCPIEHRSNPISDREKLVYKRIAIFISTVVLLITILSL
                     SLNIFVDYFTYSTFAMFWIFIMLVLGKLKAKI"
     gene            42301..42447
                     /locus_tag="PC523_000758"
     CDS             42301..42447
                     /locus_tag="PC523_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDF2474315.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKKN"
     gene            42747..44126
                     /gene="scfB"
                     /locus_tag="PC523_000759"
     CDS             42747..44126
                     /gene="scfB"
                     /locus_tag="PC523_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HDF2474316.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVYYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSITPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            44155..44652
                     /locus_tag="PC523_000760"
     CDS             44155..44652
                     /locus_tag="PC523_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDF2474317.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKRYIDESYEWYLEAA
                     QNQKY"
     gene            44950..46614
                     /locus_tag="PC523_000761"
     CDS             44950..46614
                     /locus_tag="PC523_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDF2474318.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERIKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIEIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGILRTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKIYSEYNTKKILVMEKVNGTKLSDVEKIKRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLMEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNIEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            46627..49077
                     /gene="recJ"
                     /locus_tag="PC523_000762"
     CDS             46627..49077
                     /gene="recJ"
                     /locus_tag="PC523_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDF2474319.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKSAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYHVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQNEIPVAFAVINPKQEDCNYPFDSLCGCGVAFKIIQALTPKEEFKTSMYD
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILKEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLSIENGEATGSARS
                     IKGFNIFEALVKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NINVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     NIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRGTFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            49090..49602
                     /locus_tag="PC523_000763"
     CDS             49090..49602
                     /locus_tag="PC523_000763"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDF2474320.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKGVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            49676..51883
                     /locus_tag="PC523_000764"
     CDS             49676..51883
                     /locus_tag="PC523_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDF2474321.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            51908..52357
                     /locus_tag="PC523_000765"
     CDS             51908..52357
                     /locus_tag="PC523_000765"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDF2474322.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAAKPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            52372..52986
                     /locus_tag="PC523_000766"
     CDS             52372..52986
                     /locus_tag="PC523_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2474323.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHIDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGNFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            53077..54633
                     /locus_tag="PC523_000767"
     CDS             53077..54633
                     /locus_tag="PC523_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HDF2474324.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFNDFNANNNMESKNLIN
                     RLIYENGVLFSSTVYYENGNLKYESIQNIENMNLEFLDGFDGNKTIEEFLNSLDNESK
                     RNFKKLCKESIKKSMFVVLKQIFNSYVPWGILTGIRPVKIVHNLMDKNLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKIIRETNKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIREFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLDEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            54623..55885
                     /locus_tag="PC523_000768"
     CDS             54623..55885
                     /locus_tag="PC523_000768"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDF2474325.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            55959..57746
                     /gene="aspS"
                     /locus_tag="PC523_000769"
     CDS             55959..57746
                     /gene="aspS"
                     /locus_tag="PC523_000769"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDF2474326.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKESFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEVIFKEVLDVELTLPL
                     PKMTYAEAMSRYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFSEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKASLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGADNIREVIAFPKNQNA
                     VCPMTNAPTLAENEQLEELSIKVDIKDNE"
     gene            58204..59436
                     /locus_tag="PC523_000770"
     CDS             58204..59436
                     /locus_tag="PC523_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDF2474327.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYVLMKCFGIKDK"
     gene            59450..60256
                     /locus_tag="PC523_000771"
     CDS             59450..60256
                     /locus_tag="PC523_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HDF2474328.1"
                     /translation="MGRKVKIGIIQQHSVLGDVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATEYNLESLGGVKTLELIREHNKYIEKSMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFNRKGKIMGEYCKNHLWSLEAVYFKVGENVEVYEADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSNYNI"
     gene            complement(60368..61237)
                     /locus_tag="PC523_000772"
     CDS             complement(60368..61237)
                     /locus_tag="PC523_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2474329.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENTGEVKILSPAAAN
                     VLPPLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            61609..63036
                     /locus_tag="PC523_000773"
     CDS             61609..63036
                     /locus_tag="PC523_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HDF2474330.1"
                     /translation="MKLTKSIPSMLCLSIVLSLSPVSYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSGSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEELKDISE
                     IAIVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKNFIESKNISKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKKQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNTTAITQVGSGGNENAFKELKDLQVNS
                     NNKRVMYVTNTDKVNIRSDATIEASVIGALNNGDEVEVLEVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(63115..63384)
                     /locus_tag="PC523_000774"
     CDS             complement(63115..63384)
                     /locus_tag="PC523_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474331.1"
                     /translation="MRFEYYYLIQDITGILLAFIGFRMSIIGFRILSIKGISINTLLL
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMFLISIIINPRIKVSK"
     gene            complement(63387..64829)
                     /gene="nhaC"
                     /locus_tag="PC523_000775"
     CDS             complement(63387..64829)
                     /gene="nhaC"
                     /locus_tag="PC523_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDF2474332.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICVVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLINKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLKFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDNEIEELEIFDTIQKRVD"
     gene            complement(64854..66020)
                     /locus_tag="PC523_000776"
     CDS             complement(64854..66020)
                     /locus_tag="PC523_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2474333.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRIEQEIYGYTTRPDSYNESIVSWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKENDKIMIQEPVYSPFNSVVKNNSRELVISPLQKLEDGNYMMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPIAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEVSYNNGEPWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDKELESKLVQKGKVALNQGNSFGIGGSGYQRINLACPKGMLEEALRRIKNAIN"
     gene            complement(66245..67720)
                     /locus_tag="PC523_000777"
                     /pseudo
     CDS             complement(66245..67720)
                     /locus_tag="PC523_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
BASE COUNT        26829 a         7295 c        11758 g        21860 t
ORIGIN      
        1 aatagcaata gcaatagcaa tagcaatagc aatagcaata aaatttcaaa taatagtata
       61 gattctaaat ttgtgaatag tagagttaaa tcaatagata taataagagg gctatcaata
      121 gccctcatga tagtttgtaa taatcctggg acttggatga gaatgtatcc tcaacttaga
      181 catgctgtat ggcatggtgt aacacttgca gattttgcat ttcctttttt tgttatatcc
      241 ttaggtgtta caattccaat atcaataaat agtaaactaa aaaataataa atctacatta
      301 agtataatac ttagtatatt caagagaagt atcttattga tattatttgg atttttctta
      361 aattacctag gaaatcctga tttaaatact gtaagaatac ttggagtact tcaacgtatg
      421 ggactagtct attttgtgac tagcttagta tacttactac taaaaaaatt aaatgttaga
      481 agttctgtta caataattac tttcttatgt atatctgcat ttataatagt aggatattac
      541 atagtagcaa aaccttatgg gtttgagtta gaaggttctc ttgcacaatt agtggattta
      601 catttcttta aaggtcattt gtacaagcca gattttgagc cagatggttt tttaactagt
      661 atagtagcaa tttcttcagg aatgttggga tgcatgatgg gatgtgtgct tttaaaagaa
      721 aacataggag agtataaaaa attctttaaa atattagtta tgagtattat tttactaata
      781 ggtgctttta tatttaatca gtattttcca tttaataaaa gattatggtc tagctcattt
      841 gtacttttaa tggctggaag ttatggtata ttactgtcaa tattttattt tatatgtgac
      901 atcaaaaata aaagtaaaat atttacccca ataatagcac ttggaagtag ccctattttt
      961 acatatatgt gtctcgagat attaagccat gttttttgga accttccaaa acttacaaac
     1021 aaagtcgatt atccaactac cttggtagag tggactacat atgaacttat aactccttgg
     1081 gcaggtacaa cttgggattc tcttgtattt tctttattgt atgtcctatt ctgggttata
     1141 gtaatgtcta ttatgtataa gaaaaaaata tttatcaaaa tttagtgcaa tatttttaga
     1201 cgctaaagtc tttgttttgt ttaaatatac ggaaactatc aaatataatc aaaatttccc
     1261 ctcgataaaa tttaaaaacc atgccataaa agattacaaa aagtttacaa tttgtaattt
     1321 tttgtgctat aatgaaaaaa gatttacata atattgaatt tatgtaatat aaaacaagct
     1381 taagatgtat taaaaaagat ttaaagattt aaaggtccaa aaaatttagg ggggatatga
     1441 tgaaaaaaac aacaaaatta ttggcaacag gaatggtgtc tgtagcaatg gtggcaccaa
     1501 acgtagcttt agcggcggaa aatactactg caaatacaga atctaactca gatattaata
     1561 taaatttaca aagaaaaagt gtagtgttag gaagtaaatc taatgcaagt gtaaaattta
     1621 aagaaaaatt aaatgctgat tcaataacat taaattttat gtgttatgat atgccacttg
     1681 aagctacttt aaattacaat gaaaagacag attcatatga aggtgtaata aactataaca
     1741 aagacccaga atacttaaat gtatgggaac ttcaaagcat aaaaataaat ggcaaagatg
     1801 aacagaaagt tttaaacaaa gaagacttag agtctatggg attaaatctt aaggattatg
     1861 atgttacaca agagtttata ataagtgatg caaattcaac aaaagcagta aatgaatata
     1921 tgagaaaaac ttctgcacca gtaaaaaaac ttgctgggcc tactagattt gaaactgctg
     1981 ttgagataag taaacaaggt tggaaagatg ggtctagtaa ggttgtaata gttaatggag
     2041 aattagcagc agatggaatc acagctactc cacttgcatc tacatatgat gcaccaatat
     2101 tacttgcaaa taaagatgat ataccagaaa gtacaaaggc agaattaaaa cgtctaaacc
     2161 caagtgatgt aataatcata ggtgatgatg gttcagtttc acaaaaggca gtatctcaaa
     2221 ttaaatctgc tgtaaatgta aatataactc gtattggtgg agttgataga catgaaacat
     2281 ctttacttat agcaaaagaa atagataaat atcatgatgt taataaaata tacatagcaa
     2341 atggatatgc tggtgaatat gatgctctta acatttcatc aaaagctggt gaagaccaac
     2401 agcctataat attagctaat aaagattctg taccacaaaa tacatatgat tggttatctt
     2461 cacaaggttt agaagaagct tattacatag gtggtagtca aagtttatca tcaaaaatta
     2521 tagacaaaat atctaaaata gcaaaaaacg ggacttcaaa aaatagaatt tctggagcag
     2581 atagacatga aacaaatgca aatgtaatta agactttcta tccagataaa gagttgagtg
     2641 caatgttagt tgcaaaatct gacataatag ttgattcaat aacagcagga cctttagcag
     2701 ctaaattaaa agctccaata cttataactc caaaaactta tgtttctgca taccatagta
     2761 caaatttatc agaaaaaaca gcagaaacag tatatcagat tggtgatgga atgaaagata
     2821 gtgttataag ttctatagca tctagtttgt caaaacataa tgcaccaaca gaaccagata
     2881 actcaggttc agcagcagga aaaactgtag tgatagaccc aggtcatggg ggttctgatt
     2941 caggggcaac aagtggacta aatggtggag cacaagaaaa gaaatatact cttaatacag
     3001 cattagcaac tactgaatat ttacgttcaa aaggtataaa tgttgttatg actagagata
     3061 cagacaaaac aatggcttta ggagaaagaa cagcattatc aaatacaata aagccagatt
     3121 tatttacaag tatacattac aatgcatcta atggagctgg taatggcgta gaaatatttt
     3181 acaaggtgaa agataaaaat ggtggaacaa ctaagactgc agcatcaaat atattaaaaa
     3241 gaatactaga aaaatttaac atgcaaaata gaggaatcaa gacaagaaca cttgcaaatg
     3301 gaaatgatta cctatatgtg ttaagaaata ataattatcc agcaatactt gttgaatgtg
     3361 catttattga caataagagc gatatggata agttaaatac tacagaaaaa gtaaaaacaa
     3421 tgggaactca aataggcata ggaatagaag atacagtaaa ataaaataaa aaatattaaa
     3481 gcaaaacata tcatctcctg ttataatgta aaatataaca agagatgata tatttttgta
     3541 aagaatcaaa ttgtttacaa tgatgtttct tgtattgata atctgctcat tattgagtat
     3601 aatatagaaa taagtcaaaa ataagataga aataagttga aattaagaat taaaaaaatt
     3661 tttattccaa tatggaggta actatgtctg gatatactaa tgatgagtgt gaaataccta
     3721 aaattaaaag ctatccaggg gcagataaag aaattgcaag cgagattgac tatagcatag
     3781 taaaaggaac agttttattt gacttgtacc aaagaattat ggatttagtt ttatctataa
     3841 taggacttgt gataggttta cctttaatag caatatttgg aattcttatt aaaatagagg
     3901 acaaaggacc aataacttat aagcaagaaa gactaggaaa atgcgggaga agattctata
     3961 tatataaatt aagatctatg agaacagacg cagaaaaatt tggcgcacaa tgggctgaaa
     4021 aagatgatcc tagaataact aaggttggaa agtttattag gaaaactaga attgatgaga
     4081 ttccacagct gtttaatata ttaaaggggg acatgggctt aataggtcct agaccagaga
     4141 gaccgaactt tactgttcaa tttaatgagg aaatacctgg atttatcaac aggctcgcta
     4201 taaaacctgg gcttacgggt tgggcacaag taaatggtgg atacgaaatc actcctgaag
     4261 aaaaacttaa agaagatatt tactatataa aaaataggag tatattactt gactttaaga
     4321 tactctttaa aactgttaaa gtagtattga caggagacgg agctagataa ttttaaaata
     4381 ttagattact aatcaatgaa aaagctcttt aaaaagagct tttttattgt caaaaaatac
     4441 aaaaaagaaa aggaatgaga acttatgatt aaaaaaattt ctactatatt atcgttagtt
     4501 ttgttaatct ctatttctag tactatgtgg gtatttgcag atgcaaagcc caaaagagag
     4561 ttgatagaag ggagtatacc agagatttcg acagaattaa ataaaagagc atttaaggat
     4621 tctaaagaag taattcttgt aaatgaagat tcaattgtag atagtataag tgcaacacct
     4681 ttagcatatg caaagaatgc tccaatagtt gtaactaaga gtaaaaatct tggtagagta
     4741 acaagaaatt atctaaaaga actaggtcca gagaaggtta cgatagtagg tggtttaaag
     4801 gcagtatcta aagatgctga acgtaatata gaaaaaatgg gaatgaaagt agaaagaata
     4861 agaggaaaag acagatatga tacatcttta aagatagcta gagaaatgta cagaacagta
     4921 ggatttgatg aagcattttt acttagctca acaacaggat tagagaatgc catatctgta
     4981 tattcttatg ccgctaaaag tggaatgcca ataatatggg ctaaagatga ggggtttgaa
     5041 gaacaaattg atttcttaaa aggtaaaaat cttaaaaaaa tatatgcatt aggtgattca
     5101 aaagaattta ttgcagagat tgattctaac ttaaaaaata tagagggcat aaagcaaata
     5161 aataagtcta gtacaaatgt tgatttaata aagaagtttt atgatgaaaa agatataaag
     5221 aaaatctata ctgctaagtt agactttggt agtagatctg atgtaaacga atatatttct
     5281 ttgggagtag tatcagcaaa agaaaatatg ccaatactga tttgtaatga taatttaagt
     5341 cgtgcacaag ataaattctt gaaagatagc aatataaatg atgtagttga agttgggtac
     5401 acagtaggtg attatagtct atttaagagc atatttaatc taacattctt atcttgtata
     5461 gtattaatac tattattact attgattaca tttagggcac ttagatatga gtcgaaatag
     5521 tttttgattt attaaggaag cttttcatag gttaaattta cttaattaga ataatgttta
     5581 taagcagttt taaaagttct acttttagag tttaaattag ataaggagaa agaagatgtc
     5641 aaaaacagct aaagcagcat tgtggattat ggcagccact atgttttcaa aagtattagg
     5701 tttccttaga gagcttgtac ttgcaaattt ctatggaaca ggtatgtatg cagatgtatt
     5761 tgtactaaca ttaaatatac ctggtttaat catagcagtc ataggttctg cagttgctac
     5821 aacttatata cctatgtact ttgagacaaa gaaaagactt ggagatgaag gtgccttaaa
     5881 atttactaat aatgttttaa atatatgcta tataatggct atagtaatag ctattattgg
     5941 gcttttattt acagagcaat ttgttacagt atttgcagca ggatttagaa acgaccctgc
     6001 aaagttccaa gcagcaatat tatttactaa aataatgatt tcaggagttt tattcctgag
     6061 tggaagtaaa atatttagtt cgtttttaca ggtgaatgac agttttgtaa ttcctgggct
     6121 tataggaatc ccatataata ttataataat agcagcaata gcactaagtg caggtaaaaa
     6181 tgtatggata ctaccagcag gagcactatt ggctatggcg agtcaactat tatttcaact
     6241 tccatttgca tttaagaagt cctataagta taagccatat ataaatttaa aggatgagtc
     6301 cataaaagaa ttagtcaatc tagtattgcc tatgttggta ggagtagctg ttggacagtt
     6361 aaatatattt gttgatagat tattggcaac tactttgggt gatggaaagc tgtctgctct
     6421 taactatgca aataggctaa atgagtttgt tatggcatta tttgttacat ctataataac
     6481 agttatatat cctaagttag caaaaatgtc tggtaaagat aataaagaag gatttataag
     6541 tactattgta aaatcgtcaa attgtattat attggtggtg cttcctatat ctataggagc
     6601 tattatatta gcagagcctc ttgttaggat attattccaa aggggtaaat ttgatgcgtt
     6661 atcaacagac cttacatcta tagcgcttag actatattca ctaggtttat tggcctgtgg
     6721 agtaagagat gttttatata gggcatttta ctctctttca gatacaaaaa cgccaatgat
     6781 aaatggaagt atagctttaa taataaatat agttcttaat ttaatattga taagaccatt
     6841 aggtcatgca ggtattgcca tatcaactag tacatccaat ataattacag ttatattatt
     6901 atttatatct cttaaaaaga aaaatggata ttttggagga gataaaatta taaagactgg
     6961 tctaaaaagt ttagtagcat ctggtgtgat ggcagtagtc actttattga tatataataa
     7021 tctatatgca tttataggtt caggaacaat taaagaaatt atatcagttg gtgcaggggt
     7081 actaggtgga gcttctgtat atacgatatt gatagtttta tttaaggtag aggaaatgga
     7141 tttagctttt gaatttttaa gaaaaggaaa acaaaaacta cttagaagat agattcttac
     7201 tttgaaattt taaattagag tcagaaagga tgaaaatatg gattataaga ataattatga
     7261 gatgtggtta aattctcctt attttgatga gcaaacgaaa aatgagctat taagcataaa
     7321 agatgatgaa aaagaaatac aagatagatt ctataaggat ttagagtttg gaacaggtgg
     7381 tttaagaggt ataataggag cgggaactaa tagaatcaat atttatactg ttagacgtgc
     7441 aacacttgga gttttaaatt atatcattaa gactcaagga gaggaaggta aacaaaaggg
     7501 aatagttata gcacatgata gtagatacat gtctagggag ttttgtatag aagtagcaaa
     7561 gactttaagt gcttatggtg taaaggctta tatttttgaa gaattaaagc ctacaccaga
     7621 actttctttt gctgttagat atttaaagtg tgctatggga atagtaataa cagcaagtca
     7681 taatcctaaa gaatacaatg gatataaagt atatgactca gatggtggtc aaatatgtat
     7741 agatgtggca aatgatataa tagcagaagt taataagatt gatgattaca gtgttattaa
     7801 aagtatagat tttgaagaag ctttatctaa aaatttaata actatattag ataatgaggt
     7861 agatgatgaa tttataaaag ctgttaagaa acaagtttta agacaaaata taatagatga
     7921 atatggaaaa aaattgaaaa taatatatac acctattcat ggtacaggaa ataagccagt
     7981 aagaagagtt ttaagtgaat gtggctttga aaatgtaatg gtagttaagg agcaagaatt
     8041 gccggattct aatttttcaa cagtaaagta tcctaatcct gaagaaaaat cagtgtttaa
     8101 tatagctatt gaaatggcaa agagtaatgg tactgatttg ataataggga cagaccctga
     8161 ttgtgataga gtaggtatcg tcgtaaaaga ttcaagtggt gaatatatag tcttaaatgg
     8221 aaatcaagtt ggttcactct tagttagata tatagtagaa agtttagtca aagaaaataa
     8281 acttcctaaa aacaatccta caataataaa aactatagtt acatcagaac taggtgctaa
     8341 aatagcaaag gcttataatg tagattgttt aaacacttta acaggattta aatttatagg
     8401 agaaaaaata aaagcgtttg aaaaaagcaa tgatagaagc tttataatgg gatatgagga
     8461 aagctatggt tacttaattg gaactcatgc tagagataaa gatggtgttg tatcttcact
     8521 tatgatatgt gaaatggcag catactattc ttcaaaaggt atgaacttgt atgaagcttt
     8581 gctagataca tataataaat ttggatacta caaagaggac ttaaagtcag tgactttaaa
     8641 aggtatagat ggaataaaaa aaataaaaga gatgatgctt tactttagaa gtgttaaaat
     8701 agataatgtg gctgatgtaa aagtagataa aattttagat tataaagaag gtgttgatga
     8761 tttacctaaa tcagatgtac ttaaattttt acttgaagat ggctcttgga tagctataag
     8821 accttcaggt acagaaccta aaattaaatt ttactttgga gcaaattcag acaatcaaga
     8881 agatgtagaa tttaaattga acaatttaat atcttatata ttaaatgtag tggattctat
     8941 ataagaggct aaggggagat aaataatgaa agtatataat gtcataatgg ctggaggagg
     9001 aggaactcgt ttctggccac ttagtagaca agaagtacct aaacagctta taaatttaag
     9061 tggagaagat gctttaataa acgaaactat aaacagaata gattctctag caaaaaaaga
     9121 tgatttattt atagtaacca atgagaaaca attggaagct ttaaaggata tagtaaaaga
     9181 taagtgtttg gataggaata tattaccaga gccatgtgcc agaaatacag cagcagctat
     9241 aggctttgca gcatttaata taatgaaaaa atatggtgat ggtgtcatgt gtgtatatcc
     9301 agctgaccat tatataaaag aggaaaaaga atttaaatct attttagaaa aagctatcta
     9361 tatagcagaa aataatgata aactagtaac tattgggata actcctacat ttccatcaac
     9421 aggatatgga tacataaatt ttaatagaga aaacacgata gaggatgttg catatgaggt
     9481 agtagagttt gtagaaaagc caaactatga aattgctaaa gaatatgtaa actctaaaaa
     9541 gtatgtatgg aatagtggca tgttcgtatg gaaggtatct aaaatattag aagattttaa
     9601 aagatacttg ccaaaagtat atgagaaact agaagagata agtaaatact taggaacaaa
     9661 agaagaagtg gaaaaaataa aagaaatata cccaactata caatcaatat ctatagatta
     9721 tggtattatg gaaagaagta atgatgttat agtagttcct ggtgattttg ggtggaatga
     9781 tgttggctct tgggattctc taggtgcaat atatccaact gatgatgaag gtaacatcaa
     9841 aagaggtgaa aatattacta tagatactaa aaattcaatt atatattcag atgataaatt
     9901 aatatcgaca ataggtatta gtgatttgat agtagtatct acgaatgatg cagttatggt
     9961 ttgtagaaaa gataaagctc aagatgtcaa aaaaatagta gagcagttaa aagaagaaga
    10021 tagacaagaa tatatgtagg aggtgttcat ttgattccta aaaaaataca ttatgtatgg
    10081 tttggtggtc caaaaggaaa tatagaaaat atatgtataa attcttggaa agaaaagctt
    10141 ccagaatatg agatagtaga gtggaatgaa aaaaactttg atatagagaa agaaataaaa
    10201 ggaaataagt ttttggaaga atgttataaa agaaagcttt gggcatttat atcagattat
    10261 acaagaataa gagtactcta tgaacaaggt ggagtttata tggatacaga tatgcaaata
    10321 ttaaaggaca taactcctct tttagaagat aataggctta tatgtggata tgaagatgat
    10381 ggagaatata tgaatgcagc tatactaggc gtagaaaaag gacatccttt ttttaaagat
    10441 ttactagagt actatgaaaa agagattctg acttcttcat tatttacaat accaaagatt
    10501 atgactcatc ttatggaaaa gaattataaa aagattgacc aaaataatta tgaagaagga
    10561 atacgtgttt acgataaaga atatttttat ccttttggat ttaaagaaga ctttacaccc
    10621 gagtgtatta cagaaaacac ttatggtata cattggtggg gaaaaagttg ggctaaaaag
    10681 agaaattatt tcttagaaag caaacactta acaggtgtaa ataagatatg gaagtgttgt
    10741 aaaatatttg caagtaatac tttacgaagc taaaagggag aaagtaaaat gaagacttta
    10801 aacgatgata gaaatattat gattagaaaa tatctttata tatttgtttt gtttatcata
    10861 ttacttagaa agtatatgat atcatttatt gacccaaata tggatatagg gatgattaaa
    10921 tcagcgttat tttattcttc aataggaata ctaatgctac tttttttatt tgataagaga
    10981 aaaagtataa cagaaatggt actagttgga gtttgtgtct tactttattt attaaatagg
    11041 gaaggagcta tattattaat cgttttatta gctgtatcag ctaagcaaat agatgataaa
    11101 tttatagtta aaaattattt aataatatcg gcatgttttt taatggtagc tatattatta
    11161 tttaatttat ttccttcatt aatatttaat caggaagtcc actatagata tatagaaaag
    11221 atagatatgc ttgttacaag aatggatttt ggacttggta atccaaatag tgtattttat
    11281 catatggtta ctatatatgc agcatatata ttcttaagat ataaagacta taataaatgg
    11341 gatagaataa ttttatttgg ttctgcattt tttgtttatc aaaccacata tagtagaact
    11401 ggatttttta ctatattagc aggattaata tttgtagaaa taataagatg gatagacata
    11461 aaaaaaataa aaggactacc aatgcttcta aagacattac caataatact tactttattt
    11521 agtgtaatta ttggtacagt atttgataag agtactttat taaatagatt attagcaagt
    11581 aggcctaagt tctggaatgt atacttggct gaaaagggga actttctaaa accttttgga
    11641 aattcatata gtcctgctat aaaagcaact aatccgttag atagttctta tgtatatata
    11701 attagtgtac taggagtcgt tgcttgtata ttatttatgt atttgatgta taaaggaata
    11761 gaaagcttta tagaaaaaga taaaaaagca tatttagtag ctttatttat atttttacta
    11821 tactcttttg cagaaaattt attattagaa gcttcattta gttttgcagt agtattacta
    11881 ataaaagaag tgatattaaa tgataaaagg gaaatagatt tgtggaagat gaaaagtagg
    11941 aggtagtaat gttaatatca ttaataatgc caacattaaa tagatatgat gatatatact
    12001 tacttatgga cagcttagaa aatcaaactt acaaaaactt tgaacttata gtagtagacc
    12061 aaaatgataa tagcaaagtt aaagaaatag ttgataaata cattgataag ttagatataa
    12121 agtatataaa aagctctaaa aaaggattga gttataaccg aaatgtaggc atagacaatg
    12181 ctgtagggca aattataggt ttccctgatg atgattgtgt ctacgaaaat gatacattag
    12241 aaaaagttat aaatttcttt aataaaaata aagattataa aatatacagt tgcaaaacta
    12301 tggatgcaaa taaaattgat gcattcaaga aaatgtatga tggaacttgt gatatcacaa
    12361 gttccaacgt tttggatact ataacatcca taactttttt tattgatttt gaaggtaaag
    12421 actatactag atttgatgaa aaattgggtg ttggtggaga gtttggtgct ggtgaagaga
    12481 tagattatgt tttaaactta ttgagtttag gttttaaagg aaaatacttt ggagatgata
    12541 taatttacca tcctgcaaaa aaacattcta aatctaaaga aaagtatcaa aaagattata
    12601 attatggaag aggttttgga gcactttgca aaaaggaaat agtttataga aaaaattata
    12661 agtttgtaaa ggttatggta tcaaaacttg taagaaatat aggaggtctt atattgagtt
    12721 ccaatagaga ttatcatagt gccactataa aaggaagaat taatggattt agacagtaca
    12781 agttataatg aggatggtgt ttatggacaa ggttaaattt ttaataaata tgcttttggc
    12841 tttttttata tatccattta ataagcatag gtttaaaggt agaaatatct ggcttatagg
    12901 tggacattca ggggatatat acaatgataa ttcaaagttt ttttatgagt acatgttgaa
    12961 ggaacataat gatgtagaaa cttattgggt agtaaataaa gacagtaaag tatttgataa
    13021 aattccaggc aaaaaattaa taagaggaag tgtagaaaac tatctgtatt attacaattc
    13081 aaaagctata gtattttcac atgcaccatc agcagatata gcaccataca attttgctgt
    13141 acctgttctt aattattttc ataaaaaaac aataaaagtg tttttaaatc atgggactat
    13201 aagttttaaa aagagaaaac ctatgaacaa aaagttcaaa aatattatag ataacttata
    13261 taaaagctat aatatagtta cagcaagttc tgaatttgaa agaaatgtta tggtaaatga
    13321 ttggggaatg ttagatgact cagtttatat aattggaaat gccagatatg acaatttacc
    13381 aacaaatgaa gttgcacaaa ctcgagatat attatatact cctacatgga gagattggat
    13441 aaaatttagt tcaggaaaat ttacagatac agattatttt aaaaatataa tgaatttttt
    13501 aaatgatgat aaattaaata aaatattaga tgagaaagat ataaatgtaa aaatttatat
    13561 gcatcatctt atgcatgagt ttatagatga tataaaagaa aatataacag gaaaacgaat
    13621 tgtgttttta gataaagggg taactctagc aaatgaaatc agaaaatcag ctgcaaatat
    13681 aactgattat tcaagtgtag ccatagactt tttgtatatg aatagaccta tcttgtttta
    13741 ccaatttgat ttggatgaat atatggaaaa agttgattca tatatagatt taaaaagtga
    13801 aatgtttggg tctttggctt acaataatga tgaagccgtg aataaactta ttgatattat
    13861 tgaaaataat tttgaggtta tggataacca aaaaaatgaa agaaataagt ttttcagata
    13921 taatgataat aagaactgca agaggatata tgattgtgta ttaagtaaaa ttaaataaat
    13981 attttataaa tgatgaggaa ggtaatatga agaagaattt agtatctata attactccca
    14041 tgtataattc tgaaaaattt attgaagcaa ccataaaatc agtattaaac caaacttatc
    14101 aagaatggga aatgttaatt attgatgatt gctcaacaga taatagtcct aatatagtca
    14161 aatcttatat gcaacaggat agtagaataa aatgtataaa gactgagact aataagggtg
    14221 tctctaatgc tagaaactta gcactaagta aggcaacagg acaatttata gcttttttag
    14281 acagtgatga ccaatggaat agtagtaaat tagaaaaaca agtaaacttt atgttagaaa
    14341 atgactatgt aatttcattt acttcatatg aactgatgga tgaaaatgat aaaaaattaa
    14401 acaaagtaat aaaagttcca cctaatgtag attatagaag attattaaaa ggaaatatct
    14461 taggatgtct taccgttgtg attgataagt ctaaattgga ttttgaaata agaatgagtg
    14521 gtgtaagaca tgaagattat gttttatggt tatcgatatt gaaaaaaggg catattgctc
    14581 atggaataaa tgaagttcta gcgttatata gaaaatctag caattcccta agtggtaata
    14641 aaataaaagc tgctatgtgg acttggaata tatatagaaa tatagaaaaa atacctttat
    14701 acaaagctat atactacttt attaattatg gaataaacgg aatcaaaaaa agttagacag
    14761 agaggaagat ttaattaatg tataaatata aatttactgt atttactcca acgtataata
    14821 gagctcattt acttgaaaat ttgtacaatg atttaaaagc tcaaacttat gatttcaatg
    14881 attttgaatg gttgattgtt gatgatggaa gtagcgacag tactaaagag ttagttgaaa
    14941 aatttatatc agaaaataaa ttaaatataa gatatatata caaagaaaat ggaggaaaac
    15001 atactgcaat aaatctaggt gtaaaaaatg cagatggaga gctatttttc attgtagata
    15061 gtgatgatgg tttaattaaa gattctcttg aaattgctaa tgatgagtgg gattctttag
    15121 agaataaaga aggatttagt ggagttgttg gactttgtgt atatccaagt ggagatttga
    15181 tagggacaga aatgcctgaa gataagaaaa tatgtcactt tgcagaccta tattttaagt
    15241 atggcgttaa gggagataaa agcatagtat ttgtaactga tgagcttata aaatttcctt
    15301 ttccagaaag aaaagaagtg agatttttac cagaaagtgt agtatggaat gaaatgtcta
    15361 aatactataa agttatatgt gtaaataaac ctatgataat aagagattat ttggatgatg
    15421 gtttgactaa gaatatatta agtaaaaatg cattaaaagg tagagcatta gagtttttat
    15481 atttaataaa tcaaaataca tatccattaa gtagatatcc atatatgtgg ataaaaaatt
    15541 acataaattt agccagatac tcattgttgt ctgatagtca ttattttggt gaattaagaa
    15601 aagtatctga taaattgctg tatttagcat tattcccatt aggatattac aaatatattg
    15661 gacaaagaaa attagtatct aagtaaactt gttggtatag tactgctagt tgctatataa
    15721 ggagagtaaa aatatgaatg agcctttagt atcgataatt actcctgttt ataattcaga
    15781 agagttttta tcagagacga taaagtcaat ccaaaatcaa acttataaaa attggcagct
    15841 tttattagtg gatgattgtt ctaaggacaa ttcaagtgaa attataaaaa gttttcgaaa
    15901 agaagatgct agaataaaat atataaagct tgaaaaaaat tctggagctg cagtatctag
    15961 aaatgttggt attaagaatg cggaaggtcg atttattgca tttgtagata gtgatgattt
    16021 atgggatagt agaaaattag agattcagat agaatatatg ttaaaagaaa atataggttt
    16081 ttcttttaca tcttatagat atatgagaca agatgggtca aaaacgaata aggttgcaag
    16141 agctcctaag aaaattgact atgaaggatt acttagaaat actataatag gatgttctac
    16201 agtggtcatt gataaggaga ttgtaggaga gttcagcatg cctttggtaa gaaggggaca
    16261 agatactgca acctggttac agttacttaa aaaggaaaaa tatgcatatg ggattcagga
    16321 agatttagtg aattacagat tagttggaaa ttctatatca agtaacaaga taaaagcatt
    16381 aaaacgaact tggaatactt atagaaatgt tgaaaattta agtttaccaa agagtctata
    16441 tgtattttgt ttctatgttt ttaatgccat taaaaaaaga gtataagttt tatatacttg
    16501 gaggtaaaaa aatgaaaata gctatagctg ggacgggata tgtaggtttg gttacaggtg
    16561 tttgtctagc agaagtaggc aatgaaaatg tagtctgtgt tgatgtagat gaacaaaaag
    16621 ttaagtcaat gaaacttgga atagcaccta tatatgaaga aggcctagaa gaacttatgg
    16681 ttaaaaatta caatagagga agaatagatt acactacaga ctataaaaaa gcttatgaag
    16741 atgtagatgt aatactaata gctgtaggta caccagaaag aagtgatggt tcagctaact
    16801 tagattatat aaaggcagtt gcaaagcaaa tagctgaaac tgtgacaaaa gatactctag
    16861 tagtaataaa atctacagtg ccaattggta caaatgaaga gatagaagat tatataaata
    16921 gaaacttaaa gaatgatgta aaagtggagc ttgcatctaa cccagagttt ttatcacaag
    16981 gtacagcagt aaaagatact ttgcatgctt caagaatcgt tataggtgta gaaagtgaat
    17041 gggctagaga tatattacaa caaatttatg agccttttaa tcaacctata atagttacaa
    17101 atagaaatag tgctgaaatg ataaagtatg catcaaataa cttcttggct cttaaaatat
    17161 catttatgaa tgatattgca aatctttgtg aaatagcagg agctaatata gaagatgtag
    17221 ctttagggat gagttatgat gatagaatag gcagtaaatt tttaaaggct ggaataggat
    17281 atggaggctc ttgctttcca aaagacacaa aagctcttca ctggttatct gaacataatg
    17341 gttatgaatt aaaaacagta aaagcagcaa tagaagttaa tgaaagtcaa aaacttaaat
    17401 tggttagaaa agcttctaaa ctagtagaga gttttcaaga tatgaaggtt gccgttttgg
    17461 gtttgacttt taagccagat actgatgata taagagaagc gccatctata ccaaatataa
    17521 aatatttgct cgaaagaggg gcaagagtta ctgtatacga cccaattggt gtaaataata
    17581 ctaagaaaat atttggtgat tctatacttt atgcggattc tgtagatgat gcgatagaag
    17641 aggcagagat atgttttata atgacagaat ggaaagatgt attaaattat gatatacata
    17701 aatataagga aaagatgaaa aatcctttag ttcttgatgg aagaaattgt tataaagtta
    17761 aagatatgga agagttagga attgagtatt attcgatagg aaggtaaata ttatgaagac
    17821 tgtttgttat ttagctgatg ggtcaaatcc tcatacttta aaatggtgta gtttttttaa
    17881 aaataaagga tatgacattc atgtaatctc tttaaatgga ggccacatgg aaggaataaa
    17941 aatttataac tttggttcta atgtagaaga actaaaaaat gaaaatattt ctaaaaaaac
    18001 aggatactta ggttcagtaa agcagataaa gaagctagta aatgaaataa agccagatat
    18061 attgcatgca cagtatgcaa gtagttatgg atttatagga agtctacttg ggtatcatcc
    18121 atatattatt tctgtttggg gtactgatat ttatgatttc cctaataatg gttttataca
    18181 aaaacaaatt ataaaacata actttagaaa agcagactat atcttttcaa atagtagaga
    18241 tatggctaaa gaggctaata aatatacagc taaacatgta gatgtaacct tttttggagt
    18301 agatatggat aggtttaagc ctatggaagt agaaaaagaa gatgcttttg taataggaat
    18361 tataaagagt ttagaaaaaa aatacggaat agaatattta atacaagcat ttaagatgct
    18421 taaagatgaa tataaagata agaaaataat tcttaaaata ggtggttctg gaagtcagat
    18481 ggacaaccta ataaatttaa ctaaagaatt gggtatagaa gatgatgttc agtttttggg
    18541 aagaatatct ccagaaaatg taagcaagac ttttaattct tttgatgtaa ctgtgtttcc
    18601 ttctttaaga gagggatttg gagtggcagc tatagaatca gaagcgtgtg aagtacctgt
    18661 tatagttaca aatgtaggag gtcatccaga atctgtatgg gaaaatgaaa cgggcttaat
    18721 tgtagaacca aaacaaccag aagaaataaa aaatgctata ataaaactaa tggaaaatga
    18781 tgaacttaga cttagtatgg gtaagaaggg tcgtcaattt gttagagaaa attatgaagt
    18841 taatttaaac tttaatgata tagaaaaaat ttatgattct atatttgata aatataaaaa
    18901 ataaaatgaa aaatacagca aaagatagat gttgttggct atgcttaggt gcgtagcttt
    18961 tgctgtttaa aggagggctt ctagttatgc gagaaagtat agatttagag aaagaaagct
    19021 tttttaatag gctttttaaa aaatataaat ttagacattt aacactatta attctagata
    19081 tttgtagcgt actagtagcg tttcgaatgt ctttaagctt gacaggtaat ttaaatgtat
    19141 ttaccactaa tgatataata atttcagctt gtatatatat agtacttcat atagtatcat
    19201 ttagattgtt taagtgttac aacacactat ggagatatgc aggagaagaa gaaatcatat
    19261 ctatttttat ggcaacttta gcttatttaa ttccagttta tataataaat aaattattag
    19321 ggtttgatta tcctataata ttttatgtac taaatacaat atttattata atgtttacaa
    19381 gtggcgcaag aatagcctat agagctataa gaatagtcat gaataagaca tattctagag
    19441 gcaagatttc aaatatcctt ataattggtg caggagatgc aggagaaatg gttatacagg
    19501 aactaaaaag aaatccagaa cttaagaaag tagcagtggc tataattgat gatgataaaa
    19561 ataaaattgg aagaataatc cataatgtaa aaattgtagg gacaacttca aatataaaag
    19621 aggtagttga aaagtacaat gtagatgaga taatcttttc aatagcaaat atagagaaac
    19681 gtagaaaaaa agagattatt gatatatgta aaaacactaa ctgtaaaata aagacaatac
    19741 caggaattta cgaaattata gatggaaaag ttgatataaa acagattaga gaagtagata
    19801 tagaagactt gttgggcaga gagcccataa aaacaaattt aagagagata agtaattata
    19861 ttgagggaaa agtaatccta ataactggag gaggaggctc aattggttca gagctttgca
    19921 gacagattgc aggatttaat cctaaagagc ttattatagt tgataattat gaaaacaatg
    19981 cttactcaat acaacaagaa ttacttagaa aatataaaaa taaattagac ttaaagaccg
    20041 taatagcatc aataagagaa gaaaaaagaa tggatgaaat atttaataaa tataagccag
    20101 aagtagtatt tcatgcagca gcacataaac atgttcccct tatggagtcg agccctggcg
    20161 aagctataaa gaataatata tttggaactt taaatatagc tggattatct tcaaaatata
    20221 gagctaagaa atttgtcctt atatctacgg ataaggctgt aaaccctact aatataatgg
    20281 gagctactaa aagagctgct gaaatgatta tacagactat gaatgctgaa agccagactg
    20341 aatttgttgc tgttagattt ggaaatgtac ttggtagtaa tggtagtgta attccacttt
    20401 ttaagaagca aattgaagat ggtggaccag tgacagttac tcatccagat atcattagat
    20461 attttatgac aataccagaa gcagtaggtt tagttataca agctggagct atggctaaag
    20521 gtggagaaat atttgtgctt gatatgggag agcctgtaag aatattggat ttagcaaaaa
    20581 atttaattaa attcagtgga tttgagccag atgttgatat aaaaattgaa ttttctggtc
    20641 ttagaccagg agaaaagttg tatgaagaac tacttatgtc agaagaaggt ctattggata
    20701 cagagcataa aaagattttt attggtagac caatagatgt tgatagagaa aaaataacaa
    20761 aatacttaaa acttttaaga gaaattacta acaatgaaga ggttgaaaaa atagatggta
    20821 ttatgaggga attagtacca acttatatta aaccagaaga tgctaatatc aaagaaattg
    20881 caacaactag agaaaagtag aatatatttc taatttgtaa ccagggggag caaaaagaaa
    20941 caaatatgtg ctaaaaagaa acaaaagatt acaaaaagat tacaaaaaag gttgcaattt
    21001 attttgatgt aagatagaat gttaattagg aattggaaac gaaggaacaa aatgttaaat
    21061 tttcgaaagg attaggtatt tgagattatg atgaatatta aaaataaaaa acatatattg
    21121 aaaaaattta tagctatggt gttaatagca ggagttgtaa cagtagaagc aggagcaata
    21181 acagctagtg cagcagagct aactaattca cctatgagtg caacagtaga ccagtgtgat
    21241 tttttaaatg taagaagtgg agctagtgca aatgatgcag tagtaggaaa gataaacaca
    21301 ggagacaaag ttgaagtctt agaattacat tcaaatggat ggattaaaat aaaaactgtc
    21361 gacaatgtta caggatgggt aaatggagat tatttaacta ttcaaggtgg aaatgtagat
    21421 gcaaaggtac aaaatgtttt aaacttagca tttaagcaac aaggtaagcc atatagatgg
    21481 ggagcaactg gaccaaattc ttttgattgt tctgggttca cttcttatgt ctataaaaat
    21541 ggagcaggag ttaatttacc aagagtatca agatcacaag caacagtagg aaagaaagta
    21601 tcaagagcag aattaaaacc aggagattta gttttctttg gttcaggcgg ttcaataaat
    21661 catgttggat tgtatgtagg aaattctaaa ttcatacatt caccacaaac aggagatgta
    21721 gtaaaagtta ctagcatggc tccaggaact aattatgcaa gaagacttat aactgctaca
    21781 cgtgttttac aataataata gatattgatt tacataggat tgaatattaa cattaagtat
    21841 ataattacat ttaatgtttt acatagagat ttaagagttt agtaatatta aaatagtgat
    21901 gaacgttata aaaacagcaa agaagataaa tttctttgct gtttttttat aataaattat
    21961 ttcatattat aatcaaatat aaaatataaa ttttaactat attattcaat atttgacaga
    22021 taatatgaat tttagatata cttgataaga agtatatttt agaaaaaata aatttaaaaa
    22081 attaaggggg atgaaatgaa aaaaataagt atattagtat tatcattgat aatgacactt
    22141 actatgtgca gtgtaagttc atttgctgat tcgtcaaatg ataaggaaat gagagctgct
    22201 tggatatcta cagtttataa tttagactgg cctaaaacta agaacaatga agccaagcaa
    22261 aaaaaggaat acacagattt attggacaaa ttaaaaagtg taggaataaa tacagcagtg
    22321 gttcaagtaa gaccaaaatc agatgcttta tataaatcaa atataaaccc atggtctgaa
    22381 tatcttacag gtacacaagg taaagaccct ggatatgacc cattaccatt tttaatagaa
    22441 gaagcacata aaagaggaat ggaatttcat gcgtggttta atccatatag aataactatg
    22501 gcagatgaga gcatagataa attgccagca aacaatccag caaagaaaaa tccaagttgg
    22561 gtagttaaac atggaaataa gtattactat gacccaggtt taccagaagt tagaaagtac
    22621 atagtggata gtatagcaga agtagttcaa aattacgata tagatggagt tcattttgat
    22681 gattatttct atccaggagt aagtttcaat gatactgcta cataccaaaa atatggaaat
    22741 ggacaaaata aagataattg gagaagagaa aatgtaaata ctctacttag agatgtaaaa
    22801 gcgtctataa agagtataaa gcctaatgtt gtatttggtg taagcccagc aggtatatgg
    22861 agaaataaga gtagtgaccc aacaggttca gatacatcag gcaatgaaag ttatgtaggt
    22921 acatatgcag atacaagagc atggataaaa caaggattga ttgactatgt agttccacaa
    22981 ctttactggc caattggtct taaagcagca gattattcta agttagtgtc atggtgggct
    23041 aatgaagtaa aaggaacaaa cgtagattta tacataggac aaggtatata taagcaaggt
    23101 caatcaagtt atggtggaca aaatatagca aaagaaatag tgcaacaagt aacattaaat
    23161 agaaagtata gtgaaataaa aggtagtatg tatttctcag ctaaagatat agcaaatagt
    23221 agctctatac aaaaggattt aaagagctta tattctagta gcgaagaacc agttactcca
    23281 ccatctaatg ttaaagtgga gaaattaaga ggcgacgaaa ggtatgatac agcagtagca
    23341 ataagtaaaa aaggctggcc tacaaattca gatacagtag tattagtaaa tggatactct
    23401 atagttgatg gtataacatc aacaccactt gctacatcaa atgatgctcc tatactattg
    23461 gtaaataagg ataatatacc tacatctact aagaatgagt taaaaagact taatccaagt
    23521 aaagttattt taattggagg aaatagctct attggagata aagttgagag tgagataaaa
    23581 gatacactgt caaatgtatc aataaataga gttggtggtt cagatagata tagtacatct
    23641 ttaatgatag caaaagaatt agtaaaaaat aatccagtag aaaaactata tataacaagt
    23701 ggtacaggtg aagcagactc tctttctata gcttcaaaag caggagaaga aaagcaacca
    23761 atagtactag tatcaaaaga taatgtaagt gatgaagttt acaattggat atcagactta
    23821 aaagtaaaag atgcttattt tataggtggt aatctaagta tatctgattc agtaataaat
    23881 aagttggata aagtcattac aaatgatgtt tctaaaaata gaatagctgg agaaaataga
    23941 gaagagacaa atggaaaagt aatacaaaaa ttctatccaa atgctgaata ctcatcaatg
    24001 tttgtaagta aaagtaatca attggtagat gcattaacat caggtccttt agcagctaaa
    24061 ttaaaatcac cagtagttat gcttggaaat agcgtaacat ctgctcagaa aactgcttta
    24121 gagcctaaga aaactaatct agtatataca gctggagatg gaataaatca aaatacatta
    24181 aatgctttct taaacttggt taattaataa aaaagttaat ttaggataaa atttctttta
    24241 tagatgtaga ttaaaatgaa tattattaag aaaaattaga aaataaaaaa gaactatatg
    24301 cattttgtaa tcaagtgtac acagtagctt gaaaataatt atgcgatata gttctttttt
    24361 actaattaat cttatacttt gtaataaact ttatattaaa ttttttattg tttaaactct
    24421 atgtcattaa atataaaatc atgtaatatg tctaaagtat ctgggtcaaa tctaagaacc
    24481 catccagctt ttccatatat accacctgta ctatgaattt catcatcaat aggaaattca
    24541 aattgcttaa tatttaagtc ccccattttt aaaacttgag ctcctaaaga aagtattgca
    24601 ttaggcttca tgttagtttt aacatatgga agaactgcat ctaataatcc tggatattta
    24661 gtaacaggta aagtttctgt tttcttgatt atagcttgta taatctgtct ctgtcttcca
    24721 tctctagcaa aggctgtatc attgtgtctg atacgtgcaa aagatagtgc ctgatatcca
    24781 tttagagtct gctttccagc gtttctaatg tattgtatgc tacctttatc atcactttta
    24841 ttccatttat aagtctcagg aataaattta tttagttctc gtatttctcc tttttcaatg
    24901 gtaacttcaa cgccaccaat agcatcaatt atatacataa atgattcgaa attaacacaa
    24961 gcgtaatttt gaatatcaat attaaaattc tcttcaattg tttgtattaa taaatcagct
    25021 tgtccataag cataagcatg agtaagtttt tgtttaccat gaccagggat gtctacatag
    25081 ctatcacgag ctaatgatgt aagtttgata tcattgtgct tgttatcaat agtaagtatc
    25141 atcatagcat ctgaccttga agaatcttca tttggtctag catctgttcc cataagtagt
    25201 atattaatta tcccatcttc atttttatgg tcattgttat ctaataaatc actacttatg
    25261 ctcgagtcat gaatagcaga taatttataa taaaaatatc catagacgct tatagggaat
    25321 attactacca agaaagccag tagtataaca aatttcttta attttgacaa aagtacccct
    25381 tctttcttct ttttcctgta tattttttaa tagcatacac ttaattatat taaagtattg
    25441 aaatatattc aatacttgtt tcagttacaa aaaaaagaca atgatataat ttataagaaa
    25501 atttatcgac ttatatatac aattaaaagt atatataata tattttttaa aagtaattta
    25561 tattttaaaa taaaaacaca ccccgtaaat gcaaaataat ttgcatttaa gaggtatgta
    25621 tttatcatca aattattagt ttgttttgaa atttctaatc ttcaaccata atgtctttaa
    25681 atatgaaatc atgcattgca tctagttcat attcctcaaa aggtattaca tagcctgcat
    25741 tacctatttt tctaccattt tcagtagaaa gaggaaattc catagatttc aaatctaaat
    25801 ttcctatact taaaagttct tttcctaaag atattatttc attaggattc atattagttt
    25861 ttacatacgg aagtattgta ttaactagac tagggtattt agtgactggt aaatccttaa
    25921 caccatttat taaactctgt atgacacttc tttgtcttct atctctttca tctgtactat
    25981 cgttttttct tatacgagaa aatgcaagtg tttgatatcc atttaatttt tgctcaccag
    26041 aatttcttat atatttaaga gaacctttat tgtcattttt attccattta taagtttcag
    26101 gtataaattt atttaattct cttatttcac tcttatcaac agtaacagta actccaccta
    26161 gagcatcaac tatatccata aaagagaaaa agtcaactac agcgtagttt tgtatatcaa
    26221 gttggaagtt ttcttctata gtttctatta ataaattagc ttgtccatat acataggcat
    26281 gtgttaattt ttgttctcca cgtcctggta tattaacata agtatctcta ttcaatgaag
    26341 ttagtttaag acttttattt ttattatcaa tagtaagtat catcatggca tcagaacgag
    26401 aaacttcttc tcctggtctt gcatctgtac ctaacaataa tatgtttgtg atattttctt
    26461 cgtttttata gtcattacta ctaagcgcct ctgtatttat actatcatct tgtatagttt
    26521 ttaatttagt aaaaacatat ccatatatac taacaggaaa tgctataact aaaagggcta
    26581 gaaatataac gaactttttg agtcctgaca ataatatccc ctactttcta aattttttaa
    26641 ttacacatag ttaattatat taaaaattta ctacataagc aataaaaaac acaataaatt
    26701 agcataagtt tgtcataata taattatttt tatagtattt tatagaagaa agtgtgtagt
    26761 tattattaat tttattggaa tataattgta tgaagaatta aatttttata aactggtgtg
    26821 tatattgaaa aaaatatttt cctgaatgta gttatagcat cttattgaaa ggtgtaaatt
    26881 gtaatttaaa atttttagtg tataataaga attgaaaatt taaactgata aaaattaata
    26941 aaaaataatt atagattaat aatttggggg aggaaatgta tgataaaatt aatagcaaca
    27001 gatttagatg gaactttgtt agatgaaaag agcgaaataa atccagagtt ttataaagta
    27061 tttaaaaaat taagagagag aggaataatg ttttctgctg ctagtggcag gcagtatcaa
    27121 aatcttataa aaaaatttga agctataaaa gatgatatga tgtttatctc agaaaatgga
    27181 acattggtag tatataaagg aaaagagata ttatcaaatc ctttggataa agaattagta
    27241 aatgaaatta tagagacaac aagaagcata gaaggtaaga aaatcgtaat gagtggtaaa
    27301 aaatacgctt atatagaaag taaagatgaa gcctttatag atgaagttag tacttactat
    27361 gcaaagttta aagttgtaga tgacttgact aaggtagaag gtgatatatt aaagatagcc
    27421 atatttgact ttaaaggagc agagcataat aatagcatct actttgaaaa gttttctgat
    27481 agagctcaag tatgtatttc tggagtagaa tggcttgatt taactgcaaa aggtgcaaat
    27541 aaaggaagtg ctataaaaaa agtacaaaaa atgttagata taaaatatga agaaactatg
    27601 gtatttggcg accaattaaa tgatgttgaa atgatgaaaa gtgcttatca tagttatgcc
    27661 atggaaaatg caaatgagta tttgaaagaa attgctagat tcagagcaaa aagaaatact
    27721 gagaatggcg ttgtagataa gataaaagag gtcatgaaaa taggatagtc atatgtatgg
    27781 atgaaaagat tattttgaga aactaaaaaa atatcaatgt aattttgata aataaattta
    27841 tttatgtgaa aattacattg atatttaata aacatcaata agaatcaata agagatattt
    27901 atttttttat tatactcagt atcttgtttt ctatttgagg tgcattttca gcagaaatta
    27961 aataaacata tctacctgcc ttctcaatta tagtattatt tgctatttca gaattgattt
    28021 tttctccatg tccttctcca aaaggtgctt gtatcatatc ttttttataa ttttctagag
    28081 ttttatagat tttatctacc tcgtcagttt ttatgacaat aatatcttct agagataatt
    28141 tttcagaaga attaattaca aaaccttgaa tgatgtattc ctccacatca ttaaaatagt
    28201 taaagtcctt tgcatcaaag atttttgatt cttttataag taaatcagaa gattttaatg
    28261 cttttttaat ttttgatgta gaaacgtttt tataatcacc acttgaagaa caactaacca
    28321 ttcctaatga taagattatc gaaaaaaaca atattaaaat ttttttcatg ataaacctcc
    28381 aagtttttta aactctgtct taaattagtt tactacataa ttatataaaa ataaatgtat
    28441 ttaatgttac agttttgatt aataatgtaa tattttgata ataataccta ttaagaggtg
    28501 attagaaatg agaataccaa aacatgtagg aattatacca gatggaaaca gaagatgggc
    28561 aagtcaaaat ggtatgacaa aagataaagg atatgaaagt ggaatagacc ctggactaga
    28621 gtcttataga ttatgtagag atttgggctg tgaagaggta actttttatg gatttacaac
    28681 tgataataca agacgtccgt cagtgcagac aaaagctttt acaaaagcat gtattgatgc
    28741 tgcaaatata ttaatagatg aaggtgcagc tttactagtt gttggaaata caaaatctcc
    28801 aatgtttcca gaagaattat taccatatac taatagaatt aaagctaaag aaggtcaaat
    28861 aaaagcaaat cttctggtaa attacgggtg gtattgggac ttaaataata tatcaggagt
    28921 agaaaatgta aataaaaata atatacagga ttacatacag tcatttgatg tatccagact
    28981 agacttgata atcagatggg ggggaagacg gagattaagt ggtttccttc ctgtgcaatc
    29041 aatttatgca gacttttatg ttatagataa ttattggcca gattttaaga gtgacgatat
    29101 aaaggatgca tttagttggt atgatacaca agatgtcact ttaggtggat aattaaaaat
    29161 attctgattt aaggttgtag agtgttttaa attattgaaa aaaaaacttc ttgatggtaa
    29221 aataacacta ataatatgta aaagtgtaaa aatttatacc aagaagggtg aagaaatgtc
    29281 aaaaggcaat aataacaata attcaagaaa caaatctaaa aagaccagtc atctgaatag
    29341 aaaaaagcga aaattaaata agaaaaagtt agcagtgttg atttgcttta cagttttatt
    29401 tttatttatt gcatttaagg ttactcaagg tgtagtagct ttggtaaaaa gtatggataa
    29461 aggtaataaa actagccaac aacaaaatgt taactcagaa caatttgacc ttggtaatga
    29521 agaggaaaat aaaaataaaa aatatactgt atttatagat ccaggtcatg gtggcaatga
    29581 taaaggaact gaaagcaaga ctagtcatag atatgaaaaa gacctaaatc tacagattgc
    29641 aaaaaaatta gcaaataaat tatctaaaca aaaggatatt caagtggtag tatctagaac
    29701 tgatgataca tatatatcat taaaggatag agctatctta gcaaataata gttctgctga
    29761 tgtgcttgtt tctattcatt taaatgcaga gaaaaatggg aatacagcaa caggtataga
    29821 aacttggtat aggaataaag ctacagacgg ttcaaaagaa cttgcacaga cagtacaaag
    29881 tactatagta tcctatgtta aagttagaga taggggaata gttgaaaata actttgaagt
    29941 acttagagaa tcaaacatgc ctgctatatt gatagagtgt ggatttttaa caacaccttc
    30001 agatgaacaa aaaataataa atgaaaaata tcaagaacaa cttgcagaag gaattgttca
    30061 aggtgtgctt tcatacttag attctaaagg gaataagtaa gttaacttta ttttaatttg
    30121 aggataaatt taaaaattta atgtgtagat tgtattaact ttatttcgta atgttggaaa
    30181 cgttgtttga atttagaaac tatgaaatta gaaattattt tatctaatct aatttcatag
    30241 ttttttttgt aaaaaattaa taattataaa aatttaaaaa aataaattta gcatagtgta
    30301 atgtggttat ttatatgcga ttttaacttg cattgatttg ctatcaattg tgctttataa
    30361 ttaatatata aagtttaact gtaagataat ttaaaaaata ggggaattta tatgaataaa
    30421 aatagtatga caaaaattta cagtttaaat aaaataattt ttattataat tggaatttta
    30481 ggaatagttt tacttacaag acatattgga gtaggtgtta aggttttaat tttaatttta
    30541 ttaatcttat ttagtataaa tgtatttatt tcacatctta aaattcaggt gtatgaggat
    30601 aaaattaatt taggactaat aaataatcca gacaagttta taaatattac taaagatgag
    30661 aacttcctaa aacatgttca aaactatatc attagtaatg aaaaagaaaa ctgtgtaatg
    30721 gcttgttttg atttatgtag actcaaactt ataaatgata tatatggtta tgagctaggg
    30781 gatgaagtat taaataatat tttaagtaac ttaaaatatt attttggaaa agaagctatt
    30841 tatgtaaagt taaaaagtgc tgtttttgta ttaataataa aacttgaaga tagggaacag
    30901 aagattcctt atttagtaaa tcttattaag aataaaattg tgatactaaa tcagattgat
    30961 aattttaaaa tggatataaa tattgaggct tctattggaa tctataaatt taataataat
    31021 gaaattgata taaaaaaagc tatagataat gctgatatgg caaggattaa atcaaaacga
    31081 ctaaagcaca tagagtatgt agagtttgat aatgctatgg aagaagaaat acaatcaatt
    31141 atgaaaatag agcgagattt attcttagct attaaaaata aagagtttgt aattcactat
    31201 caacctaaag ttgattcaag tacaggtaac attataggtt cagaagcgtt aataagatgg
    31261 ttgcatccat cattaggtat gattgcacca aataagttta tacctatagc tgaaaaaaat
    31321 ggtctgataa acaatattgg tagatggcta attcaggaag tttttggtac cattaacaag
    31381 tggattttag aaggtataaa tgtcttacca gtttcaataa atctttccag agtagaatta
    31441 tataaaaatg atttagttga tttttttaaa ctaatgttta atacatataa tattcccaaa
    31501 gaacttatag aattagaaat tacagaaaca acagcactaa gagatgtaaa atttataagt
    31561 gaaaggcttt atgagattaa atctttaggg atgaatatat cattagatga ttttggagtg
    31621 ggcaattcaa attttatcaa tcttaagggg ataccactag atgttataaa aattgatagg
    31681 tcacttatac tggatatagt tacaaataca aaaacaaagt ttatggtgaa ggcaattgtt
    31741 aacctttcac atgatttaaa tgtaaccgtt atatgtgaag gggtagagga tatgaatcag
    31801 gttaaagttt taagtgaact aggatgtaat ttcatacaag gttatgtatt ttttaaacct
    31861 ttagatgaga ttaattataa gagacttctt actgacggaa gttatataaa tctggaagat
    31921 actcttttag ataaatgcat ggcagttgag gaggagtaag gatgatttca gaagatgtag
    31981 attattcaat aagaatcata agagctatat ttaataatgg agaagataat ccgatagatg
    32041 ctaaaatgat attaaaaaat gaagaaatac caacactaac actatacaga ttgcttagaa
    32101 aactttgtgc gaataatata cttactttta ataaagaaga tggtaagaaa tacatgttaa
    32161 atgtatcatt ggatacaata agcttaagaa ctattgtaga agcaattgat gggaaaatat
    32221 atataaacaa atgtacgaga tatgtatcta attgcagtaa ttgtttaaaa ggttgtaaag
    32281 taaatcgcga attgttagaa atagaaaaag aaatttataa acttttatcg agaaaatcta
    32341 taagacaaat actagaataa aaaattttca gctatgataa taattggagg ttttcggtta
    32401 tggatgatat atctcaagat aattttctac tgtctaaaga gtatgagaat agcttagatg
    32461 tagatactaa aaaagcaagt ggaatatatt atactcctaa aatcattgta gattatattg
    32521 taaaaaaaac tttaaaaaat cacgatataa ttaaaaatcc atatccacga atacttgata
    32581 tttcctgtgg gtgtggaaat tttcttttag aagtatatga tattttatat gacttatttg
    32641 aggaaaatat atatgaattg aaaaagaagt atgatgaaaa ttattggact gttgataaca
    32701 tacacagtca tatattaaac tattgtatat atggtgctga tattgatgaa aaagccataa
    32761 gtatattaaa agatagctta ataaataaaa aggtagtaaa tgatttagat gaatctgata
    32821 taaagattaa tctattttgt tgtgatagtc ttaaaaagaa gtggcgatat aagtttgatt
    32881 acatagttgg taatccacca tatattggtc ataaaaagct tgaaaagaaa tataaaaaat
    32941 ttctcctaga aaaatatagt gaagtttaca aggataaagc tgacttatat ttttgttttt
    33001 ataaaaagat tatagatatt ttaaagcaag gaggaattgg tagtgttata actccaagat
    33061 atttcttaga aagtctatca ggtaaagatt taagagaata tattaaatca aatgtcaatg
    33121 ttcaagaaat agtagatttt ttaggtgcaa atatatttaa aaatataggg gtatctagtt
    33181 gtattctaac ttttgataag aaaaaaacaa aaaagacata tatagatgta tttaaaatca
    33241 aaaatgagga tatatgtata aataaatttg aaatactgga agaacttcta aagagtagca
    33301 aattcgaaca ttttaatata aatcagagat tactatctga cgagtggata cttgtaaata
    33361 aagaggatga aactttttat aataagatac aagaaaaatg taaatacagc ctggaagata
    33421 tagcgataag ttttcaagga ataattacag ggtgtgataa agcatttatt ttaagtaaag
    33481 atgatgtaaa attgaacctt gtagatgata aatttttaaa gtgttggata aaaagtaaga
    33541 atataaataa atatgtagta gataaaagtg aatataggct aatatactca aatgatatag
    33601 ataatgagaa cacaaataaa aagatactag atgaaattat agggatatat aaaacaaagt
    33661 tagaaaacag aagagaatgt aaaagtggaa taaggaaatg gtatgaactt caatggggta
    33721 gagaaaagct tttcttcgaa agaaaaaaga taatgtatcc ttataaatca aatgaaaata
    33781 gatttgctat agattatgat aacaattttt caagtgcaga tgtatattct ttttttataa
    33841 aagaagagta tttaggtata ttttcatatg aatatctagt tggaatttta aattcaagta
    33901 tttatgataa atactttaaa ataactgcta aaaaaatgag taaaaatata tatgattact
    33961 accctaataa ggttatgaag ataaggattt ttagggataa taattatgat gagatagaga
    34021 atttatcaaa gcaaataata tcaattttat taaataagtc tatagataaa ggaaaagtag
    34081 aaaaattgca gataaaaatg gataacttga taatggattc tttaggtata taagcagttg
    34141 taagtgtgaa aaaattataa tttgttaatt gtttatttaa attcacaaat aaaaagttga
    34201 ccatataaaa aatattaaaa atatatactt ttcaatattt ttttgataaa ataagaatga
    34261 atttgtgcaa ttttattttt ctccaataac taaaaatagt tttttgaatt aatatttgct
    34321 gaggaataaa aatcagaaac gtttttgata tagtaggtga taatatgaaa ggattagttt
    34381 tagaaggtgg aggaacaaaa ggagcctacc aaataggtgc atataaagct ttaagagagc
    34441 ttggtataga gtttcaagga gtaactggca catcaattgg tgctttaaat ggagcatata
    34501 taattcaaaa tgatattgaa gtaatggaag atatttggtt aaattatgac tatactcatt
    34561 ttatgaatat tgatagagaa acctatgaga gatttaaaaa tgtagatttc actactaaaa
    34621 atctcaatgc agtgtttgaa cttataaata aagctagaaa aaatgaaggt atagatataa
    34681 gcccattaag acaactaatg gaaacaacat taaatgaaga cataataaga aaatctaaaa
    34741 aagactttgg gcttgttacg gtatggtggg ataaaaagat taatccgtat ccattgtata
    34801 ttgaagatat acctaaagga agattgattg attatttaat tgctagttcc agtttgccta
    34861 tttttcagac gaatatgatg gatgataagt tgtatcttga tggaatgttt tgtgataaca
    34921 tgccaatagg actcttggaa gataaaggtt atgaagatgt tgttgtaatt cggttgattg
    34981 atgattttat tgggaaaatg aatctaaatc gacatcaaaa tataaatata aaaactataa
    35041 taccatcaga atatctagga ggttctctta ataaagatag ggataatgta gaaaaaaaca
    35101 ttcgactagg ttacttagat actatgaaag catatgaaag atgtactggg attaagtatt
    35161 attttgatgt agagtataaa tataatgaag aatattgttt tgataaattt agaagtatga
    35221 gtaatgaaac aatacaatat atttgtaatt tattgaatat aaaaagagat gtttcactta
    35281 gaactgttat ggaaagtata ataccaaagc ttggtgaagt tcttggattg ccaaaggatt
    35341 tttcgtataa agatttgttt tactgtatat atgaaaagaa acttgaagaa aataatataa
    35401 acaggataaa attatatgat tttaacaaag ttatagatgt tgtaaataca aatattgatt
    35461 ctaatagaaa gttagcttct aattttgaaa ttagacctaa aattagtatt aaaaagaaca
    35521 aaaacacaaa attattgaca aattgtatta taaaagacat aaaaaatcaa agttaaataa
    35581 aaaacaaaaa aaaatttgta attatactcg aaaaaagtgt atattagtag tataatattt
    35641 ttgtagcact agtgggaaca aaaaatgacc ataggacaaa aaagtaccac ttagtaccat
    35701 taaagtaaaa aacaggaaaa caattttaaa aaatataaaa tgtaaagttt ataggaggat
    35761 ttaaaaaatg gaaaaagtag ttagtatcaa aaatgcaagt ggattacatg ctagaccagc
    35821 aggaatgttt gttaagaagg cagctgaatt taaatcaaca gtagaggttg tagcaaaaga
    35881 caaaactgta aatgcaaaat caataatggg aataatgagt ttaggtttag ctcaaggaga
    35941 agaattaaag ttagttgtaa atggagaaga tgaagaagta gctttagctg caatggttga
    36001 attagttgaa agtggatttg gagaatagat ataataattt tttaaaattt gatatttaga
    36061 atctaatatt acttaaagta aagtaacaga gaagtattaa acatatacag accaacattt
    36121 gtagatattt aatttctttg ttactttcat gtttatttaa tcagaagatt aagactattg
    36181 ttaattgggt ataaaaatac aaatatttta caaaatgaaa taagatattt ttaatcataa
    36241 attcagaatt aaaatacagt aacaaatagt ttataaaatt ttataaaaaa ttataagtaa
    36301 gtaaaactat aaaaatgtaa attatttata taaatttata gcatttatgt gctagatgat
    36361 attttttagg aggtagggtt atggcttaca aaggaatcgg tgcttctcct ggtgttgctt
    36421 tagggaaagc gttagtagtt gaacatagtg aattagtaat tgaaaaaaaa tctatagata
    36481 atgttgaagc tgaaattgct aaattagaaa atgcagttgc agtttctaaa gatgaattag
    36541 taaaagttaa ggaaaaggct tctgaagaat taggagcaga ggaagcagaa atatttgaag
    36601 cacatttatt agttttagaa gacccagaat taattggttc tgcaatagat aaaatcaaaa
    36661 ctgaaaatgt aaatgcagag tatgctttaa atgaaataaa agagatgttt gttagcatgt
    36721 tcgagtctat ggacaatgaa tacatgaaag aaagagcggc agatataaaa gatgttacaa
    36781 atagaatact tagacatata ttaggaatta aagttgttga tttgtctgct ttaagcgaag
    36841 aagttgtatt aatagcacat gatttaactc catcagacac agcaactatg aataaaaaaa
    36901 tggttttagg atttttaact gatataggtg gaagaacttc tcatactgct atcatgtcta
    36961 gaactttaga aatagctgca atcgttggat taaatgatat aacttcaaaa gttaaggatg
    37021 gagactttgt agttttcaat ggggacacag gtgaagtaat cgtgaatcct gataaagaga
    37081 ctataaataa atatactgaa ttaaaagcta aatatgaaga tgagagaaaa gcattacagc
    37141 tattaaaggg aaaaccatct gtaactttag atggtaaaca tgtagaatta gctggtaaca
    37201 taggaacacc taatgatata gaaggtctaa taaagaatga tgctgaaggt gttggtcttt
    37261 atagaacaga atttttatat atggatagag attctttccc aacagaagaa gtacaatatg
    37321 aagcatacaa agctgtatta gaaggaatgg atggaaagcc tatagttata agaacattgg
    37381 atataggtgg agataaagaa ttaagttatt taaatatgga acctgaaatg aatccattct
    37441 taggttacag agcaataaga ctttgcctag atagaaaaga tatatttaag actcaattaa
    37501 gagctttata tagagctagt gttcatggaa gattaagaat catgttccca atgatttcat
    37561 ctttagaaga gttgttacaa gctaaagaag ttgtaaaaga agttttagca gaacttgatt
    37621 ctgaaggtgt ggcttatgct aaagatgtag aaattggtat gatgatagaa gttccatctg
    37681 ctgctgtaat atctgatgta ttggctaaac atgtagattt cttctctata ggaactaatg
    37741 acttgattca atatacttgt gcagttgata gaatgaatca aaagataagt tatctttata
    37801 atcaatttaa tccagctgtt ttaagactta taaagactgt tatagacaat gcacataaag
    37861 aaggaaaatg ggcaggtatg tgtggagaat ctgctggaga ccaaaaaatg ataccaatac
    37921 ttttaggtat gggattagac gagttctcta tgtctccaat atcaatactt cctgctagaa
    37981 agttaataac ttcagttaaa gagtcagata tgaaaaaatt agctgatgag gtacttaata
    38041 tgggtactgc agaagaaata aaaagctata tagaaaagac ttttaatata taattaatgt
    38101 tatatattta ctaaaattta tttttttaaa taacaaaatt atattttaca aattaaaaag
    38161 gagatgtttc aaaataaaag tttgaagcat ctctttttta taaaaaatta ctaagaatta
    38221 cttaatctgt gtaatagtcg ttttgtgaaa aaaacaaaaa tatattatga ttgcagtaga
    38281 tatggatgtt aggagaaata aaagtactat taaaagtaag attcgtctta aaattccatt
    38341 aaaagcagtt aagatggagt aatataataa tcttatgact ataataaaaa ttaaaaaaat
    38401 atggtattga aaaataatta aaagtattta ataatagaat tagggaagaa taagtttata
    38461 agtaaaaata attgattagt agttaataaa ataaatgtga tatccaaata aatcctaaat
    38521 ctaatttgag ttttaatata gatagtctaa agtttgatta ttaaagattt aattgtaagt
    38581 tgttacagtt gatgatattg attagagatt aaagtttaga atagaaagtt accttaaatt
    38641 taaaaataaa agataaccca atctaaaaat tataatcact cgataggttt aaggatttat
    38701 ttgtttatta taaataagag ggtgtctcaa aagcaacttt ttagtttatg gggcactctc
    38761 ttttacatga aattaaatat attttgttgt ttcaaaaata aaaaagaagc ttatctctat
    38821 tttgagatag cttctttgtg tactattatt aatttaatca gtaatataat ttaaagttat
    38881 aatactagtt aagatttact gctttattag taaataattc tttaatagca ttgtttctgg
    38941 aaactaaatc attatcagag ttgtttgttt ttttcataat cactgaataa cgttttttat
    39001 ttataaaaaa tattaaaatc ttatcattat caaaattgta ttctttatat ctatcaatag
    39061 actcataacc tagatttaga atgtttttat cttttatact atctacatct aaaatatatg
    39121 atgggtcagc ctttagtaat atattaatta aacatttttc ttcaccagca cagaataact
    39181 ccatcttact tgatatagat ttagattgct ctgaagttat ttttgcagga ataaggttgc
    39241 cattatcatc tcttagaaga taaccatgtg tgtcttgttg atatactaaa ttaacttttt
    39301 tcttaggaac actaatcatt ttatctaaat gtacataatg atgattttca acaaaacctt
    39361 cattcaatat ttcaacaaag ttatcaattt tacctaggca aaaagattct atagattctt
    39421 tacagaattt atttgtaaat ggtattatgt ttacgttttc ctcgtctgta ttccatgcta
    39481 tagcataatc taatgaatac acttcttcct ctatatctgc aaaattatat tgtactatct
    39541 ttcctttcaa aattactcga cctccatctt ataatatatt taatatactt tataaaactt
    39601 atattatatt taaataatat cataaattgg ataaatatga tatagtaaat aagaaaaagt
    39661 ttgaaaaaaa tcactataaa tgagtaataa atataaatag tgtatactaa tgggatggaa
    39721 aatgtccact attaacttta ttaccccgca tatataagat aaaattatta atataaaaat
    39781 ataatctatc aacaatgaat agacttttaa agaaacttaa tataaaatta attaaataag
    39841 tgcttaaaaa cgatttactt taatgataga atcatattaa aggaggataa ttttatggat
    39901 ataaagaata aaattaaatc tatattttta ccagaaaaaa atgactattt agatgatgaa
    39961 gaatttgagt atgatataga aaataatgag gaagattcaa aggaagataa caaagtacat
    40021 ttaaatgatt taagcagagt aaaatatgat tatagagagt taaaagacat agaaaaccaa
    40081 acagaagaga tgtgtctgct agcagtaaag caggatggta ctataattga gtttgtaaag
    40141 gacaagactt ataaagtatg tatggaagca gtaaaacaga catataaagc tcttaagtat
    40201 ataacaaatc aaaatgagga tatttgtata gaagcagtaa aacagaacta cagagctttg
    40261 tactacataa ataataagac agaaaatgta ctaattgaag ctataaaaaa tgcatcaact
    40321 tatgatgtta tggaagtatt taaatttgta gaagagcaga cagaagatgt atgtctagca
    40381 tttatagaaa gagcatctaa aaatgatgtc gcagagatac taaaaggaat aaaagagcaa
    40441 actccagcta tatgtttaga agctgtaaaa aaagacggaa aatctttagc ttatgtaaag
    40501 gaacaaagta atagtatatg tttagaagct gtaaaagaaa actacagtgc actgtcttgt
    40561 gtaaaagagc aaacagaaga aatatgtata gaagcagtaa aacagaatga ctttgcattg
    40621 tattatgtaa atgaacagac agaaaaaata tgtatggaag cagtaaaaag aagttatatg
    40681 gctctacagt atgtaaataa gcaaacagaa gagatttgtt tggaagcagt aagaattgat
    40741 ggaagagcat tacagtacgt aaaagagcaa acagaagaaa tttgcttgga gtcagttaga
    40801 caaaatggaa aagtactgca atatgtaaaa aaacagactg aaaatatatg tatagaagca
    40861 gtaagaggtt catttgaaga acttgaaata aaagaaatat taagttatgt aaagatacca
    40921 acagaaagaa tatttgtaga agcagtaaag caaaatggaa aaattcttaa atatgtagaa
    40981 aatcagacag aattaatatg tttagaggca gtaagagaaa actataatgc tttagcttat
    41041 gtaaaggaac aaacagaaga gatttgttta gaagcagtta atcaaagtta tgaagcgctt
    41101 aaatatgtga aagaacaaac agaagaagtt tgtctaaaag cagtaaaaca agattataga
    41161 atgttgaaat atgtaaataa tcaaaccgaa aaaatatgtt tagaagcagt aaagcaaaat
    41221 tatagagctt tagaatttgt tgataatcaa acagaaaaag tttgccttga agcagtaaaa
    41281 cagaatagaa aggcactaca gtatgtaaaa caaaaacaaa gttaaaaggt taaaatatat
    41341 ataaaatata tgcaaacaaa taaaaattaa tattttgtaa taaataaagt gggtatctca
    41401 aaataagctt tttagtttgt gaggtactct ttttgtatga aataaaatat atttttagtc
    41461 ttttaacaat gaaaaagagg gttatctcta ttttgatata ttctttttat gcatataaag
    41521 cttaataaaa ttgttttatt ataattgttt tattatacaa ataataataa taaaattata
    41581 taggtctttt tttgaccagt tttacatttt taacaactag ttttgtaaag tatattgaat
    41641 tttctcaaaa aagaatgtac tctatatatg taaaattttt ttggaggtaa aaatgtttaa
    41701 aagatatgct gaaaaaatga catctgtttt aatttgtaat aatgtgatag ataataatga
    41761 atctaaagtc tattcttatg gatttgaaat attaatagct ttcgtagtta atatagccac
    41821 catgctgttt ataggttttt tatttggtaa atttacatat gtattgtttt ttttaatgtg
    41881 ttattgtcca ataaggcaat tttcaggtgg ttatcatgca gacaattatt ttagatgctt
    41941 attaaccttt atttttataa ttttatctac aatcttaatt atagaaaatc taaatataga
    42001 cttatttaaa aatattatta tgattattgc aagtgttagt tgggttggaa tttgtgccct
    42061 atgcccaata gaacatagaa gcaaccctat aagtgataga gaaaagttgg tttataagag
    42121 aattgctata tttatatcta cagtagtgtt attgataaca attttaagtc tttctctgaa
    42181 tatatttgta gattatttca catatagcac atttgccatg ttttggattt ttattatgtt
    42241 agtattaggt aagttaaaag ctaaaatata agtaaaatat gtatgaggga gaattaagtt
    42301 atgaaaaagt ttattgtaag atttatgaaa tttgctagct cattagcact tagcactgct
    42361 attttgtcag ctaatagtac ctgtccttgg attattcatc aacctaaagt acccaaagaa
    42421 atcagcaatt taaagaagaa aaattaaata caagcaatta tttatacaaa attattaaaa
    42481 aaaattatta tattttgata agattaaaac aagataaaat cagatacaaa atttatttta
    42541 tttattttat aggagcctaa tacctatttg ataatatatt agtgcgccaa tttaggggga
    42601 tatagtgtat tcaatgaaat gacttacggt gagtaagtca tttttgtatt gtatgtattt
    42661 tgggtaaact tatgatataa tgaagggtaa agtttaatta aaataataaa aaactttagt
    42721 atagatagta atttaggagg taaggtatga gtatgataca taaattttct atgaatggat
    42781 ataatatcgt tttggatgtg aatggtggag ctgttcatgt gttagatgat gttgcatacg
    42841 atttgttaga tttttataaa gaaaaaagta aagaagaaat attagaaata ttaaaatcaa
    42901 aatatcaaga agaaaaaata aatgaagcgt atgaagaaat attaaatttg gaaaaagaag
    42961 gtcttttata tacagaagat acatatcaat atcatccaag ttttgtacat agagaacctg
    43021 tagtaaaagc tttatgttta aatgtagctc acgattgtaa tttaaaatgt aaatactgtt
    43081 ttgcagctca aggtgatttt ggaggagaaa aagagcttat gagttttgaa gttggtaagg
    43141 cagcaattga ctatttaatt gctaattcag gaagtagaaa gaacttagag atagatttct
    43201 ttggtggaga gcctttaatg aattttgaag ttgtaaaaca actggtttat tatggaagaa
    43261 gtgtagaaaa agattataat aaaaatataa gatttacaat aactacaaat ggtgtattgt
    43321 taaatgatga gattatagat tatataaatg aaaatatgca caatgtagtc ttaagtttag
    43381 atggaagaaa agaagttaat gataacatga gaccaacttt aaatgataaa ggaagttatg
    43441 atattacatt accacgattt aaaaaacttg tggaaaaaag agcaaaagat aagtattact
    43501 atataagagg tacttttaca agagataatt tagatttttc aaaagatgtt atgcattttg
    43561 cagatttagg atttaaatta acctctgtag agccagtcgt tggagatgag agcaatccat
    43621 atgcacttag agaagaagat ttacctaaga tatttgaaga atacgaaaaa tttgctgtag
    43681 agtatgcaga tagacaatta caaggagatg gatttaaatt tttccatttt atgatagatt
    43741 taaatcaagg tccatgtgta ataaagagaa taactggatg tggggctggg aatgagtatc
    43801 tttctataac tccaaatgga gatatctatc catgtcatca atttgttgga aatgaagaat
    43861 ttaaaatggc aaatatattt gatgaagaaa ttgtacttcc agaaaattta aaaaatatgt
    43921 ttagagaagc gcatgtatat actaaagaag agtgtaagca atgttggaat aagttttact
    43981 gttcaggtgg atgtcatgca aatgcgataa actttaataa tgatatatca aagccatatg
    44041 aattgggatg tgaaatgcaa agaaagcgta cagagtgctc tataatgata caagctaagt
    44101 tgatgttaga aggagcaacc aactaaattt atattttaaa ggagttgttt atatatgatt
    44161 agagattatt tagaagataa gcctttaatt gatgaaagtg tatttgtggc aaaatcagca
    44221 gatgtaattg gaaatgttaa aataggcaaa gattcaagta tatggtacaa tgcggtagtt
    44281 agaggtgatg aaggcccaat aactattgga gaaaatacta atatacaaga ctgttctata
    44341 gtgcatggag atacagaaac tataattggt aacaatgtaa cagttgggca taggtcaata
    44401 gttcatggtt gtaaaattag tgataatgta ttaattggta tgggttcaat aatattggat
    44461 aatgctgaaa taggagaata tactctgata ggagcaggta cgctaattac atctaataag
    44521 aagtttccac caggtgtttt gataatgggc tctcctggta aagttgttag agagcttaca
    44581 gaagaagata aaagatatat tgatgaatct tatgagtggt atttagaggc agcacaaaat
    44641 caaaaatatt agtttaaaga gtggttgttt caaaacatat ttaaatctgt tttgaggcaa
    44701 ccactaattt attttattag attttgttga tattttagtt tttaaaaagt atttgtttaa
    44761 atcagatata aaatgtcaaa aataaaaatc aactgagtta aataatttta ataaaattgc
    44821 tagtatatgt gataaaatta aatattaaag agtatataag atattagaat atcaattggt
    44881 gaaataatgt catatacgat aaattaaggt gtaagttttt gggtatacct atcttaggag
    44941 tgaaaagatt tgagaataag ttatagaaat ttaaagcgat atagagaaat cggatatgtt
    45001 ttaataaagt atggttttag ttttattgta gaaagactaa atatagaagg tattgcctat
    45061 aaaataccac tatttgaccc accagaagaa ataaaaaata tgacaactgg tgagagaatt
    45121 aaaagagttt tagaagaact aggacctaca tatataaaaa taggacagat attgagtaca
    45181 agaaaagact tgttagacca agatataatt gatgaaatat caaaactcag agatgatgta
    45241 gagaaatttg actcaaatat agctattgag atatttaaag aagaagtggg tttaagtata
    45301 gaagaaatat tcttagaatt taaagaagaa ccaatagcag ctgcttcgat tggtcaagtt
    45361 tatgaaggta tacttagaac aggagaagaa gtaattgtaa aaattcaaag accaaatata
    45421 gaaaaaataa taaaatctga tttagagatt cttagaacta ttgcaaatac tttaaaggat
    45481 ttaaaaaaag attttaattt agatttagta caaatgattg aagaatttca aactcaatta
    45541 atgagagaac ttgattacac atttgaagct ataaatgcaa ctaaattctc taggatattt
    45601 aaaaatagcg atgaagtata tatacctaaa atatactcag aatataatac taagaaaatt
    45661 ttagtaatgg agaaagttaa tgggacaaag ttaagtgatg tagaaaaaat aaagagacta
    45721 ggttataaca caaaaactat tgttgaaata ggagttaggt cattttttac acaggtatta
    45781 tctcatggat tttttcatgc agacccacat ccaggtaata tattcgtagt tgctaaaaac
    45841 aagatagcat atatagattt tggaatgatt ggaattattg ataataaaac tttaaaccaa
    45901 ttaaatgaaa ttgcattagc aggtgtagaa aagaatgttg ataagataat atatctattg
    45961 atggaaatgg atgcattaaa tggagaggct gatattaagg gacttagaca ggatttactt
    46021 tatctaatac attattacta tgatatttct atagaaaaaa taaatgtaac agatatatta
    46081 aatgaactat ttagattttt tagacagtac aaaatagtca tgcctgcaca atttgtaact
    46141 cttgccaaga cagtcataac attagaagga acaagtagaa ctttaaatac agatttttct
    46201 tttggctcta tgggaaaaga gtttatgaaa catcattata agagtaaatt taaccctaaa
    46261 aatgttgttt taagttcaag acaaaatata gaagaaatat tgctggacat aaaaactatt
    46321 ccaaagcaat taaaagcaat tttaaaaaat atagaaagaa ataacataaa aatgcaaatc
    46381 gaagatgtta aaatgacaag attagaaaat tgtataatag agttaacatc tcaaatatct
    46441 ttgagtttag tgttggcatc cattatagta ggttcttctc ttataattgc atctccaaat
    46501 atagaaaata atatatggat aaagtttact gcgatagcag gtttctttat atcctttata
    46561 ataggtcttt gtttagttat aagaagtatt aggtcaaaat acaaaaaaga ttagggggaa
    46621 ttaaatttga aaaaatggat attgaaatat aaaggtaaga tagaggaaag tgaatttagc
    46681 aaaaaactta atatttcccc ggaaatctgc caaattttaa aaaatagagg gatatgtact
    46741 gaaaaagatt cagaaatatt tatgaatcca tctttagatt atttaagaga ccctttttta
    46801 atgaaagaca tgcaaaaagc tgttgataga ataaagtcag cagttgagaa gaacgaaaaa
    46861 atttggattt atggagatta tgatgttgat ggtgtatcat ctacatctat cttgtgctta
    46921 tattttaaga gtataggtta ccatgttgat tactatattc ctaatagatt agaagaagga
    46981 tatggtataa atgaagatgc tattaagctt ataagctcta ggggatgtga cttgattata
    47041 agtgttgact gtggaattac atcagtgtct gaggtcaata cagcaaatga tttgggcata
    47101 gatgttataa ttacagacca tcatgaatgt caaaatgaaa ttccagtagc ctttgcagta
    47161 ataaatccaa aacaagaaga ttgcaattat ccatttgatt cactatgtgg atgtggtgta
    47221 gcatttaaaa taattcaagc cttgactcca aaagaagaat ttaaaactag tatgtatgat
    47281 tatttagaaa tagtaacact agcaactatt tgtgatattg ttccacttat agatgaaaat
    47341 agaataattg taaaaaatgg tttaaaatca atgaaagaag gtaaaaatat tggattaagg
    47401 gagcttataa aagtctgtgg cgtagaaagt gacaagatag gttcttctca tataggtttt
    47461 gcaataggtc ctaggataaa tgcctcaggt cgattggggt attcttactt aggagtagag
    47521 ctatttacta ctcaatcaca ggaagaggca gtagaaatag ctagtatatt gaaagaaaaa
    47581 aataatgaaa gacagatgat agaagctaag atgtatcatg aagctgaaga aatgctaaag
    47641 tctaatagca gatacaatga tgacaaagtg ttggttttag ctaaagaagg ttggcaacat
    47701 ggaataatag gaattgtagc atcaaagtta actgaaaaat actacaagcc aacaattctt
    47761 ctaagtatag aaaatggaga agctactggt tcagcaaggt caataaaagg atttaatata
    47821 tttgaagctc tcgtaaagtg caaagatttg atgactaagt ttggtggtca tgaacaagca
    47881 gcaggattat ctttggattc agataatgta gagattttag caaatgaaat aaataaattt
    47941 gcagattata atttaactga agatgatatg atagagaata taaatgttga gtttgaatta
    48001 caagaaaatg taataaattt aaatcttgta gaagaattac ataagttaga accttttgga
    48061 ttaaataatc ctaatcctag atttatagtt agaaattaca ttttaaaaga tttgaaagtt
    48121 ataggtaaga atcaacaaca tctgaaatta aacatagaaa aagagaaaag ctatgaatgt
    48181 ataggattta atatgagcca tttaaaatca atgtataaag taggagacaa agtggatgta
    48241 ttgtttcaat tagatgaaaa taactatatg ggaaatagga aagtacaatt tttacttaaa
    48301 gatattcgat tggctcgacc taaaagtgca agtaatgaca aactttcatt gaaattaatg
    48361 tcaaaaataa taccaaaaga tactcaaagc ttgtataata tatctgtatc tgattttgaa
    48421 ttatttgatg gaaatacaga tataaatata tttgattatt ttgaaaaaga tacactaata
    48481 atatctaatt ctataaatgg attttataga gcaatgtcag atatatcact tatagatttg
    48541 gattttaaca taaattacaa tattattgaa gatgttagca aaaatactga taaattagag
    48601 cttatatttt ctcctaatat tgataaaata gacttaaaaa gatataataa tattattctt
    48661 tatgattatt tatacaataa aggcgaatat tcgtatatat atgagaataa aagagaagaa
    48721 agtgaaatta taaaatatta taataaaaca gacctattat atttaaagaa tgtagttagt
    48781 aatatagttc catcaagaga tgaatttata acaatttata aacaagcact cataaaaaaa
    48841 gaaattgatt tggatatggt aaatataagg ggaactttta atgtaattcc attaaagttt
    48901 tttacgatat taaatgtgtt tagagaatta aatttacttg actttaattt aaattatgaa
    48961 aaaaattctg tactcataag aatattgcca aaaccacaga aaaaattaga cttaaatgag
    49021 agtttaatac tcaataatct aaaaaattta gaaaaacaat acaatagcag ttattaggag
    49081 gcgtttccca tggatttaaa aaactttata aggaatattg atgatttccc aaagccaggt
    49141 atagatttta aagatgtgac aactctattt aaagatggag atgcatttaa atatgcagta
    49201 gattctatag ttgaagaatt gaaagataaa ggtgtagatt tagtaatagg accagaagca
    49261 agaggatttt tgatgggtac acctgtagca tatgctttag gagtaggatt tgttccaatt
    49321 agaaaacctg gaaagttacc aggtgaagtt gagagttatg agtatggatt agagtatggt
    49381 acagatactt tagagataca taaggatgca attaaaaagg gtcaaaaagt agcaatagta
    49441 gacgatttat tggctactgg tggaactatg gaagctgctg ctaagcttgt agaaaaatta
    49501 ggtggagaag tagtttctat gcagtttttg attgagctaa aattcttaaa cgggagagaa
    49561 aaattgtcta actatgatgt taattctctt ataaaatact agtactttta taatagttgg
    49621 tataacacca actattttca tatatattaa aattttatac aaaataggtg gttatatgca
    49681 tgataaagaa ttacaagaaa taattgaaaa aataaaagtt tatgcaccta atgcaaattt
    49741 tgaaatggtt gaaaaagcct ataatcttgc aaaaaaagcg catgaaggac aatatagaaa
    49801 atctggggaa ccatacttta ttcatccatt agcagttgca aatattttaa tagagatgga
    49861 attagatatt gaaactataa cagcaggttt acttcatgat gttgtagaag atacagaata
    49921 tacctatgaa gacataaaaa aagaatttgg caaagaagta gcggatttag ttgatggagt
    49981 aacgaaactt ggtcaaataa aatatcgttc aaaagaagaa actcaatctg aaaatttaag
    50041 aaaaatgttt ttggcaatgg caaaggatat aagggttata cttataaagc ttgctgatag
    50101 attgcacaac atgagaacat taaaatacat gcctccagaa aaagcaaaat ataaagcaaa
    50161 agagacgctt gaaatttatg gaggtatagc tcatagatta gggatttcca agataaaatg
    50221 ggaactagaa gacagagcat taaggtttat ggacccagaa gggtattatg atttagttag
    50281 tagagtatca atgaagagaa gtcaaagaga agattatatt caaggaattg ttgaacttct
    50341 taaagataag tttaaagatt taagcatacc atgtgatgta tatggtagac caaagcattt
    50401 ttacagtata tatagaaaga tgcaaaaaaa acataagact tttgaagaaa tttttgattt
    50461 aacggcagta aggatattgg ttgataatgt aaaggattgt tatgcagtac ttggtatagt
    50521 tcatacatta tggagaccta ttccaggaag atttaaagat tatatagcaa tgccaaaacc
    50581 aaatatgtat caatcattac acactactgt tgtaggtcct gatggagaac ctttagagat
    50641 acaaataaga actcatgaga tgcataatat agctgaaaat ggtatagcag cccattggaa
    50701 atataaagaa ggtatctcta gcaaagaatc aaaagttgaa gaaaaactcc aatggttgag
    50761 acagatgatg gagtgggaaa aagatttaaa agacccacaa gaatttatgg atgcacttaa
    50821 agaagatgta tttaatagtc aagtttatgt atttacgcca aaaggagacg taattgaatt
    50881 accagcagaa tcaacaccta tagattttgc ctatagagtt catacaaatg taggtaataa
    50941 atgtgtagga gctaaaattg atggaagaat agttcctatt gattataagc ttcaaaatgg
    51001 taatattgtc gaggtgctta catcatctaa ttctaatgga ccaagtagag attggctcaa
    51061 tatagtgaag acgccaaatg caaagagtag aattagacaa tttttcaaaa aggaaagaag
    51121 agaagaaaat gttgaccgtg ggaatacaat attagaaaga gaatttaaga agcatgatat
    51181 accgcaaaaa gaccctcttg tcgaaaaata tatggcatta gttgctaaga aattcaatca
    51241 gccaagtgtt gaagatttaa ttgccactgt aggttatggt ggtataatgt catcacaagt
    51301 tgtatctaaa gtaaaagacc tgtatttaaa agaggttaaa aaagctaaaa aagaacaaca
    51361 gatacaagaa gaagatttaa ataaacacaa tataagtgaa acagaataca gtaaaaagag
    51421 aaagaaaaac gcatcacaag gaattatagt aaaagggctt gacaatatct tagtaagatt
    51481 tgctaagtgt tgtaatcctc tcccaggaga tgaaattgta ggatatatta ctaaaggtag
    51541 gggagtggcc atacacagag ctgattgccc aaatgcaaac aataatggcg atttcttcca
    51601 aaatagattg gtggaggttt catggagttc agcaggaaag ggtaagtttg aagcagaaat
    51661 tcaaataaaa gctattgaca gaagaggaat tgttaatgag ataactcata ttgttgcaat
    51721 ggataaactt agtttaaatg gaataaatgc aagaaaaggt aaggatagta ttgtaagtat
    51781 aaacttactt gtagaagtta atgacacaga ggaattaaaa attctaatga aaaaattaaa
    51841 agcaattcct ggtgtagaaa gtatttatag aatgacaaat tagagttaaa ataataggga
    51901 ggaagctatg agagcagtag tacaaagagt atcttcatct aaagttacag tagatgaaaa
    51961 cactataggt caaataaata aggggttact agtattatta ggagtgactc atgatgataa
    52021 atcaagtgat gtagattata tgattgacaa aatattgaat ctgagaattt ttgaagatga
    52081 aaatgacaag atgaatttat ctctaatgga tataggtgga gagcttttag tagtatctca
    52141 atttacttta tatggagatt gcagaaaagg aagaagacct ggatttagta atgcagctaa
    52201 accagaactt gcaaataacc tttatgaaga atttgtaaaa aaagcaaaag ataaaggtgt
    52261 gacagttggt acaggtcaat ttgcagctca tatgatggta gagttaacta acgatggtcc
    52321 agtaacaata ctattagact caagtaaatc tttttaaggg ggaaaacttt aatgattata
    52381 aaaaaatttg tagatagtta ctttggagta aatacttatg tattaggtga tgaaaagact
    52441 aaaaaatgtg cagttataga cccaggtgga agtttagtag agacattaag ttttgtaaaa
    52501 gaaaatgaat taaatattga atatataatc cttactcatg ggcatggaga ccatatagga
    52561 tatgttaagg atattaaaga aaaaactggt gctaaggtag ttgcacatat tgatgaaaaa
    52621 gaattattaa atgacaaaaa taaaaatcta agttatacaa tgagatgtgg agcacaagaa
    52681 tttgatgcag acatatatgt gaatgataaa gacaagcttg aacttggtga attaaaaatg
    52741 actttcttgc atactccagg acatactcaa ggatgtatgt gtataagagt tgaagatgaa
    52801 atgttcacag gagatacttt gtttgctgga agtattggta gaactgattt gtatagtgga
    52861 aactttaatc aaatggaaaa atcacttaaa aaattatgta aatatgaaga tgctgttaga
    52921 gtatatcctg gacatggacc aactagtact ctaggggttg aaaagaagac aaatccttac
    52981 atgtaatttt atattagtat ttaaaaaagt ataaaatatt taataactgt attagtgatg
    53041 gggttcttct actgttaaat agaaggagtt aagttgatgt tgtatgtttt tttaaaagga
    53101 catgattata agtatgaagt agcagaactt ataaaattgt ttaccagtga atttaagttt
    53161 aacgacttta atgccaataa taatatggag tcaaagaatt taataaatag attgatttat
    53221 gagaatggtg tattattttc aagtactgta tactatgaaa atggaaactt aaaatacgaa
    53281 tcaatacaga atatagaaaa tatgaatctt gaatttttag atggatttga tgggaataaa
    53341 acgattgaag aatttctcaa tagcttagat aatgaaagta agaggaactt taaaaagctg
    53401 tgtaaagaaa gtatcaaaaa aagcatgttt gtagtactta aacaaatatt taattcttat
    53461 gtaccatggg gaattttgac tggtattaga ccagtcaaaa ttgtccacaa tctaatggat
    53521 aaaaatctag atgataatag cataagaacc attctaaaag ataattattt tataatagat
    53581 gagaaaatag atttagctct tgaaatagct aagagagagc gattatttat gtatcctata
    53641 gataaaaata aaatttctct ttatgtaagt attccatttt gcccaactag atgtgtatat
    53701 tgttcatttc cttcaaactc attaaagcaa tttggacatt taaaaagaga atatgtttat
    53761 aaattgattg aagaaattaa ggggtttgcc aagattatta gggagactaa taaagaagtt
    53821 gaaactctat atattggcgg tggaactcca actacacttg atgaggaaga attggatttg
    53881 cttataaatt cattgtttaa tgaattagat ttaagtaaaa ttagagaatt tactgtagaa
    53941 gcgggtagac ctgacaccat tacggaacaa aaactaagag tgttaaaaaa acacaatgtt
    54001 agtagaatta gtataaatcc tcaaactatg aatgatgata ctttagtaaa gattggaaga
    54061 gaacatagag tatctgattt agtagattgc tttaatatgg ctcgtagaat gggatttgat
    54121 aatataaata tggatataat tttaggtctt atagatgaag atttaaatat ggtaaagaac
    54181 acattggatg agataaaaaa actttctcct gaaagtctta cagttcatac attagccata
    54241 aagagggcat ccaacttaaa tataaatatg gatagatata aagaacatct aacacagtat
    54301 gaagaaatgg taaaaatgat agatttatca atgaaatatg caaaagatat gggtttaaat
    54361 ccatattata tgtatagaca aaaacatatg ttgggaaatt tagaaaatat aggttatgct
    54421 aaagaaggat atgaatgcat ttacaatata caaattatgg aagaaaaaca aagtaactat
    54481 gcattaggtg caggtgctat atcgaaattt gtttatgtag atgaagatag aattgaaaga
    54541 gtagaaaatg taaaaaatgt tgaacaatac attgaaagag tagatgaaat gataaaaagg
    54601 aagaaagaag aggtatataa aaatgttgac taaagctcct agaggaacta aagacataac
    54661 tccaaaagag gcttataaat ggcgttatgt agaaaataaa tttagagaaa tatgtgcttt
    54721 atatggatat gaagaaatgg taacacctat atttgaacat acagaactat tcaagagaag
    54781 tgttggagac actacagata tagttcaaaa agaaatgtat tcttttaaag ataaaggtga
    54841 tagagaaatt actctaaagc cagaaggtac agctggtgta gtaagagcat ttatagaaaa
    54901 taaattatat gcagatactc aaccaacaaa attattttat gttacacctt gctttagata
    54961 tgaaagacca caagctggta gacaaagaca atttcaccaa tttggaatag aagcacttgg
    55021 aagcgatact ccttcaatgg atgctgaaat aattgcacta gctgttcaat ttttcaatga
    55081 agttggatta aatgatttgg tagtgagtat aaattcagtt ggttgtccag tttgcagaaa
    55141 agagtataat gctttattaa aagaatatct tgattcaaaa gctgatatat tgtgtgatac
    55201 ttgtaatgag agaagagaaa aaaatccaat gagggttata gactgtaaaa atcctacatg
    55261 taaagaaaat atcaaagata taccatttat tgcagaccat ttgtgtgatg attgtaaatc
    55321 tcactttgat aaacttcaag aatacttgaa ggaaatgaat ataaactttg taattgataa
    55381 aacaatagtt agaggtttag attactatag aaaaactgct tttgaaatta tatctaatga
    55441 tataggagct caaagtactg tatgtggtgg aggaagatat gatggtcttg ttgaacaact
    55501 aggaggacca aaaggtataa gtggaattgg atttggacta ggaattgaaa gacttttact
    55561 tacacttgaa ggtaatggaa ttgaaataga aaaccctcaa tctacagata tatttatagt
    55621 aactataggt gaagaagcaa atacaagaag ctttaaatta ttaaaagact taagacaaaa
    55681 tcatataagt gcagataaag accatataga gagaagtgtt aaagcgcaat ttaaatattc
    55741 agataaaata aattcaaaat ttacaatagt aattggagat gacgagctta aaaatgacac
    55801 tgcgacatta aaaaatatga aaacatcaga gcaaactact gtaaaattaa gtacattagt
    55861 tgaagaatta aagcaaaagc tgtaaaataa gaaagtatga tgttttgtgt attagataaa
    55921 tatatagcat taaaattaaa taaaatggag gtagtatctt ggaaacttta aagggtttaa
    55981 agagaactca ctactgtgga gagttgagag aaaaaaacat aaatgaagaa gttgtactta
    56041 tgggatgggt acagaaaaaa agaaatttag gtggtcttgt atttgttgat ttaagagata
    56101 caagtggatt atgtcaaata gtttttgata cagatgtaga taaagaatct tttgaaaaag
    56161 ctgaaaaact aggggcggaa tttgtaatag ctgtaaaagg aaaagtatgc gaaagacaat
    56221 ctaaaaaccc taatatgcca actggtgata tagaaatatt tgcaactgag cttagacttt
    56281 taaacaagtc agaaactcca cctatttata taaaagatga tgatgatgtt tctgaagcac
    56341 taagattaaa atatagatac ttggacttaa gaaaaccatc tatgcaaaga aacttaaaat
    56401 taagacataa agtaatgaat attacaagaa attacttatc aaataataga ttttgcgaaa
    56461 tagaaacacc atttttaata gctccaacac cagaaggagc aagagattac cttgtaccaa
    56521 gtagagttaa tccaggaaaa ttttatgcat taccacaatc accacaatta tataaacaat
    56581 tattgatggt aagtggtatg gatagatatt tccaaatagt taaatgcttt agagatgaag
    56641 acttaagagc agatagacag ccagagttta cgcaaataga ctgtgaaatg tcttttgttg
    56701 agcaagaaga tgttatgagt atgattgaag gtttgcttga agtaatattt aaagaagttt
    56761 tagatgtaga attgacactt ccacttccta agatgactta tgcagaagct atgtctagat
    56821 atggttcaga taaaccagat actagatttg gatatgagtt aacagatata tctgatgttg
    56881 tttgtaactg tggatttaaa gtgtttgctg atgctacaca acccggaaaa agtgttagag
    56941 gtataaacgt aaaaggcaaa gctgatgact ttactagaaa acaaatatca tcactagaag
    57001 aacatgctaa gacatataga gcaaaaggtc ttgcttggat gaaagtagga caagaaggag
    57061 taacttctcc aatagcaaaa ttctttagtg aagaagaaat gaacgctata cttacaagga
    57121 tgaatgctga agtaggagat ttacttttat ttgtagctga taaaaactca atagtatttg
    57181 atgctttagg acaagttaga cttgaagtag caaatagact taatctattg gataaaaacg
    57241 tctataattt actatgggta actgagttcc cagtttttga agaagatgaa gaaacaggta
    57301 cattctcagc aatgcatcat ccatttacat cacctatgga tgaggattta gataagttag
    57361 aagaaggtga taaagcatct ttaagagcga aagcttatga tattgtcttg aatggttatg
    57421 agataggtgg aggaagcgtt agaatatcaa attctgatgt tcaatcaaga atgtttaaag
    57481 cacttggttt tacagaggaa agagctaatg aaaaatttgg ttatttatta gaagcattta
    57541 agtatggtac tcctcctcat gctggtctag cttttggtct tgatagactt gttatgttac
    57601 ttgcaggagc tgataatata agagaggtta ttgcattccc taaaaatcaa aatgccgttt
    57661 gccctatgac aaatgctcct acattggcag aaaatgagca attagaagaa ttaagcataa
    57721 aagttgatat aaaagataat gaataatata tagttgtaga aaataaaatg taaaaagtct
    57781 ggatataaag tgtatttata ttcagatttt ttttattaga actattttat attttctctc
    57841 tatatatttt cataaattat ccttgtattc tcatccaatg taattaaaac actatgttta
    57901 aaaattttac ataaacttga ttgttttaga aactactttt gtaatgagta cacaatttta
    57961 attaaagaat atataacaaa tttgaaataa atctaatata tgactcaata gattaaatta
    58021 aaataataaa tttagactat ttacatattt ataacttaaa ttatatcaaa ttgataacaa
    58081 actagatatt atactttttt gtagaataga attacaatta tataaagaaa aattctattt
    58141 atagtgaaaa aaaagtagaa aaaagtagaa attattcttt tataaagaaa gggagggata
    58201 atattgcaag agagagaaga attagtttta aatccagttc caaaagagga aagagttgga
    58261 tggttagctc cattatttaa tatgcttggt tctaacatag ctatatctga actaatggtt
    58321 gggggaactt taattttagg aatgactctt tctaatatga ttattacatc aattatagga
    58381 aacttaatat tagttgcaat tattatgata caaggctata taggttacaa agaagggcta
    58441 aatacgtatg tattagctaa gggggcattt ggagagatag gaggaaagta tcttatatct
    58501 ctattacttg gtataactag ttttggatgg tttggtgtac aagcaggggt tgcaggatta
    58561 tcagtacaaa agatattccc gagtgtaaac ctaacattag ttacagttat acttggatta
    58621 tttatggttg tttttgcact gtatggcttt aaagctatgg ctaaattcaa ttacttagtt
    58681 ataccgccac ttatgatatt aatggtctgg ggagttttta aagctttttc tacttatgga
    58741 gttgaagcta tttataatta tactcctcaa acaactatga gtatggttga aggtcttaat
    58801 attgtagtag gtcttgttat agttggtgca attatatcac ctgaccagtt aagatatacg
    58861 agaagagtaa aagatatttg gattattagt tttttaggtt tagggattat ttccttattc
    58921 caacaagttg cagctggtgt aatgtctatg ggagcaccta cttgggatat aacagaagta
    58981 ttggctaatt taggttttgg atgggtagca tttgttattt taatacttgc atcttggtca
    59041 acaaacgtat caaatgctta ttctggtgga ttagcactaa aaactatttt tccaaatgta
    59101 aaaagaaatg ttttgacttt agttgcaggt ctaataggta cgataatagc agctactggt
    59161 ataatattta aatttcagtc atttttatct ttcttgggaa ttgcagttcc tgcaatagca
    59221 ggaattatgt ggtgtgagta ttattttata caaggaagaa cttataagca tagagaagga
    59281 ataaattgga ttgcagtaat aagctggttg ataggctttg cagcgtcgta ttatagttca
    59341 aaaataaatt tcttaatacc tccaataaat ggaattgtag tgagtatggt tatttattat
    59401 gtattaatga aatgttttgg aatcaaagat aaataaagga ggcaattaca tgggtcgtaa
    59461 ggtgaaaata ggtataattc aacaacacag tgtacttgga gatgttaaaa aaaatataga
    59521 aaaagcagta gagatgatag atgacttagg aaaacaaggg gcagatataa tttgtttacc
    59581 agaactattt gctacagaat ataatttaga gtctttaggt ggagttaaaa cattagaatt
    59641 aataagagaa cataataagt acatagagaa atcaatgtca gaagctgcta aaagaaataa
    59701 tgtatatctg atatctccat atggaacctt agaaaaaggg tctacacacg tttataattc
    59761 agcagttata ttcaatagaa aaggtaagat tatgggagaa tactgcaaaa atcatctatg
    59821 gtcattagaa gcagtatact ttaaagtggg ggaaaatgta gaggtatatg aggctgattt
    59881 tggtagattt ggtgtaatga tatgttatga tgcaggtttt ccagaagtat ccagagaatt
    59941 gacattaaaa ggctctgaaa taatatttat accatctgca tggagaatac aagatgaaga
    60001 tatgtgggat ttaaatgtat ctcaaagagc tttagagaat actgtatata cagtaggggt
    60061 aaatttagta agtaatgact caaatttaat actatttgga aagagtaaaa tatgcaatcc
    60121 tagaggtact gtaataactc aattagacac gtatagggaa aaatatgtat tatcagagat
    60181 tgatttagat gaaataaaaa agtataggga agatatccca tatttgaaag acagaaaaag
    60241 taactataac atataattct atattataat acaagttcaa tataaaatga caaagagagg
    60301 atgtatcaat attaagatta aatttattat actttgatat atcctcttta atattatcaa
    60361 cttcgattta aatattctta aaatagttga taagaaagtt tttgaataac aatacagctt
    60421 tagattcata gttttcacta aaccatgaaa gatttaaata tcttttaaat tcaatatctt
    60481 tgatatgtaa caacttaatc tcagggtctt gagataatcc ccatgatttt ccacatataa
    60541 atccaactcc ttgaagtgat tttataagag cataaattgt atatggcgaa tcagattcaa
    60601 atgctatttt tggtttaaaa ttagctgatt cgcataggat atcaattact tctctataat
    60661 tctcaccctt cgttataact ataaagtttt catctgaaac ttcacttaga tatacttcat
    60721 cctttaatga caaaggatgg tttattgaaa ctcctaatag tatttcttcg catgttaaag
    60781 ttatgctatt ttctgagttt agctttgtaa atgaagaaga tatgtacaaa tcaaaatcac
    60841 tttttttata agatgatggc aaagtatgtg acacattaaa agtaatattg ggatatagtt
    60901 ttctgaaatt actcaaaaga ggtggtaaca cattagccgc tgcaggagat aatatcttta
    60961 cttctcctgt attttctaaa gaagtgtctt gtaactcctt ttttgaactt tctaccaaat
    61021 ctaaaatgct atctactctt tttagaaaca atcttccata ctcatttaaa acaatgtatc
    61081 ttccttttct atcaaataac tgaactccaa gttccttttc taaggaagag atagttttgc
    61141 ttaaagctgg ttgtgctata tttaatttgt gtgatgcttt tgttatgtgc tccattctag
    61201 caacagtttg aaaatactta agatgcaata aatccatagt ataaaataca cctcttttaa
    61261 tatgacttta gttatatcaa ttttataatc aactccacat tatacattat aatataaagt
    61321 ttgtataata aaccacaata tattaaaaag ttatttaaat tatcatttat attttgtaca
    61381 taatctaatt aaaaaagtag ctttaatatg attaactaag gaaacattat tacaaagctt
    61441 aattacacaa aaaattcatt tgatgagtaa agtattaatg tcaggtaaga ataaaagctt
    61501 attggagaag aaaaataata ttttataata atgatattac ttaaattgtt gtgttatact
    61561 attttaaata ggaaacattt agatactata attttagggg gagtcaagat gaaattaact
    61621 aaatcaatac catcaatgct gtgcttatct atagttttga gcttatcacc agtatcatat
    61681 gcaaatgaaa taagtagaac aacagttgag aatactttag taggtcagga tagatatcaa
    61741 actgctgttg aagtaagcaa aaaaggatgg agtagtgcaa atgaggctgt agttgtaaat
    61801 agtgaatcta ttgtagatgc attggctgta acaccgtttg ctaaattaaa aaatgctcct
    61861 atattattga cagaaaaaaa taatctaaat tcacaaactg agaaagaatt aaaaagatta
    61921 ggggttaaaa aagtttatat aattggacta tctggttctg tatcaaatga tgtgcaaaaa
    61981 cagttggaat ctaataattt aactactgat agagttggtg gtagtgatag atatcaaaca
    62041 gctttaagta tagctaaaaa aatagaagaa ttaaaagata tatcagaaat agctatagta
    62101 aatgggtaca caggtcttgc agacgcagtt agtatagctt ctgtagctgc tactaatgga
    62161 atggttatac ttccagtgtc tgatgcatct ggtatatcta gttttaaaaa ttttatagaa
    62221 tctaagaata taagtaaatc atacataata ggatctacta atgctgtatc agataaaata
    62281 aaacaaagtt tacctaattc agaaagaata ggtggagcag atagaaatga aactaatggt
    62341 aaagtaatag aaaagtttta tacatctaac aaattaaata atgtatttgt agctaaagat
    62401 ggaatgaaaa agcaaaatca acttatagat gctttagcag taggagtact ggcatcaaaa
    62461 gaaaattcac ctgtactaat cgtaggagag caattaagtg caacacaaaa gagtgtattt
    62521 tcgaataaaa atactaccgc tataactcaa gttggtagtg gtggaaatga aaatgcattt
    62581 aaagaattaa aagatttaca ggtaaatagt aataataaga gagtaatgta tgtaactaat
    62641 acagataagg taaatataag aagtgatgct acaatagaag cttctgttat aggagctcta
    62701 aataatggtg atgaagtaga agttttagaa gtgttaaaaa ctggatgggt aaaaataaaa
    62761 tacaatgaag gtattggata tgttagtggc tcttatttga caaataataa gcccgataat
    62821 agtaatgaaa atataaaaat aaagtatgta aaagaaaaag atggtctaaa tgttaggaaa
    62881 ggaccaagta cagaagatga aaaaataggg catctttcat atggaagcaa agtggaaaca
    62941 atagaaatgt ttgctactgg atgggtaaaa ataaaatata atggtggata tggttatgta
    63001 agtaatgatt acttatctga tacaccagtt atttaattat aagtaacaat aaaaaactgc
    63061 tttaatttgt aaagcagttt cttttttgta aaattttgag tctaataatt atttctattt
    63121 tgatacctta attctaggat ttattattat ggatattaga aacatacaaa cactccaaat
    63181 ccagtgccta attccaaact tagatattaa taaattcagt cctgctatag taaataagca
    63241 gtatttaatt acaagtaaaa gtgtatttat agaaattcct tttatagaaa gtatcctaaa
    63301 tccaataata gacattctaa atcctataaa tgccaataat atacctgtaa tgtcctgaat
    63361 taagtaatag tattcaaatc tcatatttaa tcaactcttt tctgaattgt gtcaaatatt
    63421 tctaactctt caatttcatt atctgtatat gtaagcatag taaaaccagt tacaccataa
    63481 ataaatgaaa ttataggagt aatccaactt aagaaacaat atggaataaa ttttaatggg
    63541 cttacaccaa gagtttgagc acagaataca gcatttgaat tccatggtat taatggacca
    63601 ccaagcgtag ctgtatcttc tataattcta gatagattct gtggttttaa tttaaattct
    63661 ttgtatattg gagacattag agttcctaca aatacagaag taaacatcat tgacgaacta
    63721 aatgcatttg caaagtagct tactataaaa gtagaaccaa ccaatttagt tcttgatttt
    63781 cctattttat ttataagagg ttgaaggagt acttctaaga atccagtttt ttgaagaata
    63841 ccagcaataa caaagactac aaatactagt aaaacggttt cagccatact cattattcca
    63901 ccacgattta atagtttgtc agcatagaca aatccagtat ctatagtaaa tcctgatagc
    63961 atgtaattta aaacatcacc tattttttct ccttcttgaa atactgctat aataagtccc
    64021 atgatagctg aacttagtat agatatgata ggtggctttt gtagcaataa aagtaataat
    64081 aaaaatatta ttggaatcat tgctactaaa ccaatatgaa agtttgaatt taatacatct
    64141 ttgatttgat ttatttgaac gtagtctata gtattatttg aatacttaaa tccaattact
    64201 gtatacagaa ctacacatat tacatatgca ggtcctgtgg tataaaacat atgtttcata
    64261 tgtgtaatta catctgtctt acaaactgcc gcagctaagt ttgtagaatc tgatagaggt
    64321 gacatcttat ctccaaagaa agcaccagaa atgactgcac cagctgttaa accaagggga
    64381 atacccatac tagtaccgat actcatcatt gctataccag aagttccaag agttccccaa
    64441 gatgtaccag tagcaacaga agtcagtgaa catacgatta gtgttgtaag cagaaaatat
    64501 ttaggggtta ttattgttag tccactatat actactgttg gtattgtacc agcagctatc
    64561 caagctgcta ttagtacacc aacagaaagt ataattaagt ttgattgaaa ggcatcttta
    64621 ccaacctcaa atccaaaagc ttctatttca tcattagtat aaccaagctt catagctgca
    64681 ggaactacta taagccatga aagtaaaaac attgtagtta tagaagcatt aaatacaact
    64741 atgccaactg atgttagagc tattattgca agcattacta acaaggaata tctaaatgaa
    64801 ggtatttttc tttctgtatt cattttcata gtgtaaattc tccttaaata tttttagtta
    64861 attgcatttt ttattcttct aagagcttct tcaagcatgc cctttggaca agctaggttt
    64921 attctttgat acccactgcc tccaatgcca aagctatttc cttgatttag agcaacttta
    64981 cctttctgta caagtttact ttctagttcc ttatcactta atccaagtgc gctaaagtct
    65041 acccataata aataagtgcc ttctggtttt ctaactttaa gttttggcat attttcattt
    65101 atgtatttta tagcaaaatc tatattagat tctaaatatt ctaaaaatga ttctaaccaa
    65161 ggttcaccat tattatatga aacttctgtt gcaactaagc taaaacaatt atttctcttt
    65221 atatcaattc tagtaaaggc atcatctaac aatttatagt ctttttcatc aggtagtatt
    65281 acatatgatg attgaagacc agctatatta aaagttttag ttggagccat acaagtaatt
    65341 gtattttttt caaattcttt agaaatagat gctataggaa tatgcttatg tttttttaat
    65401 ataatgtcac tatgtatttc atcagatatg atttttacat tatgcttaag acaaatatct
    65461 cctagttttt ttaattcatc ttttgtccat actctaccaa ctggattatg aggattacaa
    65521 agtatgaata attttacatc tttaattttg ttttctatat cttcataatc catcatatag
    65581 tttccatctt ctagtttttg taatgggctt ataactagtt ctctactatt attttttaca
    65641 acactattaa aaggactgta aactggctct tgtatcatta ttttatcatt ttcttttgtg
    65701 agctcgttta ttaaaagact tatagctggt ataacacctg gactataaat taaccactca
    65761 ctttttattt tccaattatg tcttctatct aaccaactca ctatagattc attgtaagaa
    65821 tctggtcttg ttgtgtaacc ataaatttct tgctctattc tattttttaa ggaatcaatt
    65881 atacaaggtg cagctttaaa atccatgtca gctacccaca taggaagtaa atcattagtt
    65941 ccatactttt tttccatctc tgaccattta gatgaaaaat tattgcttct atcaacaatt
    66001 tcattaaaat tataattcat tgtaaatacc tccagtaatt ttaattttat gttaataata
    66061 ttaagcaaac aacgtgccaa gattgataat aaaaaatatt tattcacaag ttaatattta
    66121 atattaagtt atttcagcgt atagataaga taataatatt aattatattt aaaactttgt
    66181 ggaaaaggtt atttctaata aattttcaaa tatatgaaaa tccaattaaa taggtttaat
    66241 aggtttaact gtttaactta ttagacctgt ttatattttt tagaaattca actccttttg
    66301 atgttataat tgaaccacca cgaccaacaa gtatgtttac aagcccaaat tcttgtagtt
    66361 ctagaagtat atttctaatc tgttgttccg tcaaaaatct attttcctct aatgctattt
    66421 tatataagct ttttctgcct attctttctt ttaaatcata agcctttttt aaattatcca
    66481 gtataaatat gtattcatct agtgaccttt taaaattata gtcgtatttc ataagatttt
    66541 tgtctttacc aatgttgaat ttatattttt ttatattatc agttacttta ttacaaacag
    66601 ttctgctata atttgaatct atagtatcca atatatactc aggcaaatca catattttta
    66661 taatatcttt tcccaagtaa cagaagtatt ctcctaagtt tcttaattct cttacatttc
    66721 cttcccacct gtacatttta aagatttctt ttacttcttc acttagacta aaattacatg
    66781 gaatatcata ttttaatgaa ccaaaaattt caaatatatc agtaggtctt tccctaagtg
    66841 gatgtatttt aaggggaaga acattcaacc taaagtataa atctcttcta aacatgtttt
    66901 tagatacaag ttcttttaaa tttctattag tagctgcaat tattcttaca tcaatatcaa
    66961 tgacattatt agaaccaata cgtcgtactt gtttttcttg tatgactcta agtaatttag
    67021 attgagaatt taaatccatt tctccaattt catctaaaaa tatagttcca ttgttggcta
    67081 attcaaataa accaattttt ccaccttttt tagcaccagt aaaagcccct tcatcatagc
    67141 caaataattc actttgtaga agattttctt gaaaagtact acaatttact gctacaaaag
    67201 gaccatcttt tctcctagaa gcactatgaa tggattgagc aaataattct ttaccagtgc
    67261 cactttcacc aattataagg atagatgaat ttgattgagc cattttgtta gcaatttttt
    67321 ttgtattaat agtttgtatg ctagaaccta atatgtcatc aaatgtatat ttactgatat
    67381 taccagagtt tactagctgt gctcttaatt tagcttgctt tttttctaat tgagaaaact
    67441 ttgtcatctt cagtataaat ccatcaaata catttaattg tatatgttta atctctaaat
    67501 ttatatcaat atgatttatt ttaatcagtt tctcaaaaaa tggagtttta ttggtcaata
    67561 tgttttgaaa attaatgtct tttatgcaat caccaacaaa tgagtcaatc atttcatttg
    67621 catttataga taaaattttt ctagccatat ggttatagaa ttgaattatg ccatcattat
    67681 ttgtgcatat aattccatca tcaattatag atagcaacaa ctgaacctcc catgactaaa
    67741 gt
//
LOCUS       DAMJVM010000011        60725 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.011, whole genome shotgun sequence.
ACCESSION   DAMJVM010000011 DAMJVM010000000
VERSION     DAMJVM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 60725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60725
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.011"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(165..1016)
                     /locus_tag="PC523_000778"
     CDS             complement(165..1016)
                     /locus_tag="PC523_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HDF2474334.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHYFEQGTKIKVSYSGGVFKSGDLILESLKKY
                     LNKARFD"
     gene            complement(1126..2040)
                     /gene="murQ"
                     /locus_tag="PC523_000779"
     CDS             complement(1126..2040)
                     /gene="murQ"
                     /locus_tag="PC523_000779"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDF2474335.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAVEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVEYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKNILDRQEGYISEALKSIERL"
     gene            complement(2139..3236)
                     /locus_tag="PC523_000780"
     CDS             complement(2139..3236)
                     /locus_tag="PC523_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDF2474336.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKVAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKMKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEVNMSNNTNYIDTIMDYQPNKENLIGCHNFYPHRYSGLNLEH
                     FIKSTERFNKYGLRTAVFVTSQNESTFGPWPITDGLPTIEIHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DNLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            complement(3469..4815)
                     /locus_tag="PC523_000781"
     CDS             complement(3469..4815)
                     /locus_tag="PC523_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2474337.1"
                     /translation="MLDKFTEFLDIKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYIKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKVVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEVY"
     gene            complement(4905..5213)
                     /locus_tag="PC523_000782"
     CDS             complement(4905..5213)
                     /locus_tag="PC523_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2474338.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGTKNGIDIDVLAVGTQEFEDE
                     VKNGWDIAMVAPQVRHRFDYLKGFADEVGVPCALIKAQAYSPLGGPKLLKQVQELLSE
                     "
     gene            complement(5480..5869)
                     /locus_tag="PC523_000783"
     CDS             complement(5480..5869)
                     /locus_tag="PC523_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474339.1"
                     /translation="MSNNKKKKNNNYKNAKITKSNKNTKREMEKNQKTDYNIPTISFK
                     SGLILIVSSLVSTVIFPVLLSIFGIDNRLGVIIGNSLITSFAVAYTRYFIETKRGFSL
                     GFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            complement(5871..7031)
                     /locus_tag="PC523_000784"
     CDS             complement(5871..7031)
                     /locus_tag="PC523_000784"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDF2474340.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGSGRNTKIKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCYKANFNIENLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAHETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDNNKNVALQNIGGIGNITIIPKSCNIEDVFAFDTGPGNMIIDGVCQKLFNKRYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQSPPKSTGREIFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIRYIKELLLGVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKSKVILGNITPKPFGGK"
     gene            complement(7451..8470)
                     /locus_tag="PC523_000785"
     CDS             complement(7451..8470)
                     /locus_tag="PC523_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2474341.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDIFKNRYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYINLYNCIEDSGEIDSYLVDGQSFEENLIKDIIENLK
                     MKNNSDIESYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSNIEDSLDIKNQIAKI
                     NFLVNSILYSSRSNTMFEDLFVNKFNLDKMVRMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYILEQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGLYFVKKMIDSLGHEIEVCSENGSYTIFNIYFYDISDY
                     LNLDS"
     gene            complement(8464..9144)
                     /locus_tag="PC523_000786"
     CDS             complement(8464..9144)
                     /locus_tag="PC523_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2474342.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEIVLDEFVDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIDILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYIIQC"
     gene            complement(9296..11203)
                     /locus_tag="PC523_000787"
     CDS             complement(9296..11203)
                     /locus_tag="PC523_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2474343.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQEENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTNIRTGDITSVLPIIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMNRIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSKVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAIFYTLPMVSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            complement(11204..11956)
                     /locus_tag="PC523_000788"
     CDS             complement(11204..11956)
                     /locus_tag="PC523_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2474344.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFIAIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPAVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(12274..13428)
                     /locus_tag="PC523_000789"
     CDS             complement(12274..13428)
                     /locus_tag="PC523_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2474345.1"
                     /translation="MINKAIKFRFIIFYFLSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDKINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTSKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYNSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSVNIGYNYTLMVLSLFGCLAYGLIVLLNYLKE"
     gene            complement(13431..13898)
                     /locus_tag="PC523_000790"
     CDS             complement(13431..13898)
                     /locus_tag="PC523_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2474346.1"
                     /translation="MFFKRKKEVITINSYIDGRIINLEEIPDKVFSTKVMGDGFAMIA
                     KDNALKSPAKGVISFIAQTNHAIGIKLQNNVQILIHIGLDSATTKKENFKLKVKQGQE
                     VNMGENLVEIDNKLLENTLYIPLVIIENPNTVKYHLSNLHQEGNSGIKVINFD"
     gene            complement(13912..14400)
                     /locus_tag="PC523_000791"
     CDS             complement(13912..14400)
                     /locus_tag="PC523_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474347.1"
                     /translation="MITNKIKNVTAVLKDENIFLSVIMGCFLILPVLYIVYLELTSLF
                     TNISFSELLNNDVNASLNLVSNFSAVYSAYVIYNLKKNKDIKENMIAFYLVLLGQIFQ
                     IYTLTIVIMFTYVYKFVGIKQIISSIKELQWGKSYKALFSSVAVCLISLFIMIIRIKL
                     IM"
     gene            complement(14414..15745)
                     /locus_tag="PC523_000792"
     CDS             complement(14414..15745)
                     /locus_tag="PC523_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2474348.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVDKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGVAYGLRTIGPVIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(15759..17324)
                     /locus_tag="PC523_000793"
     CDS             complement(15759..17324)
                     /locus_tag="PC523_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2474349.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYVHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHVQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFAAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFIAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYIIQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDTEETKLYTKNEYLEAKDEKGNKLSKASQQAAE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(17493..18278)
                     /locus_tag="PC523_000794"
     CDS             complement(17493..18278)
                     /locus_tag="PC523_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2474350.1"
                     /translation="MRLEEMIFLNYNKLNETDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(18443..19642)
                     /locus_tag="PC523_000795"
     CDS             complement(18443..19642)
                     /locus_tag="PC523_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HDF2474351.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLENKDEYIKRIRSTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKEVVDRYDSMNPKIKMPDDIFVKKELILKDVIKG
                     KIGYMYLPSMSSKNGSINNDLKIIGDYISTLKKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTTARNIKLNPVESLPASVKENAPKEIIKDFNGFE
                     DTSYTIKSTDDLKFKGNIYLLVDRKIYSSSESFAMFCKETKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKSGVCDEEFKTTPEFKVKDCKRTADFKEDNCIRKAL
                     EFENINY"
     gene            complement(20164..21501)
                     /gene="xylA"
                     /locus_tag="PC523_000796"
     CDS             complement(20164..21501)
                     /gene="xylA"
                     /locus_tag="PC523_000796"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDF2474352.1"
                     /translation="MNEIFKGIGQIKFEGVKSNNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKANMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKVNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            complement(21605..23152)
                     /gene="xylB"
                     /locus_tag="PC523_000797"
     CDS             complement(21605..23152)
                     /gene="xylB"
                     /locus_tag="PC523_000797"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDF2474353.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDSEGKTLRKSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEVTG
                     YVTKDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLDEDVYDIIN
                     NQVSQVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDTIIKTSTKLEPISENVDIYKTYHRLYKKIYKSLKDDFRLLDE
                     AVNCKNK"
     gene            23360..24520
                     /locus_tag="PC523_000798"
     CDS             23360..24520
                     /locus_tag="PC523_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDF2474354.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSMSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHDSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKKLYYEDNQNTKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            complement(24676..25080)
                     /locus_tag="PC523_000799"
     CDS             complement(24676..25080)
                     /locus_tag="PC523_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2474355.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTNELVVKLEKIGKDNISMVEQNTSLLSDDE"
     gene            complement(25101..25583)
                     /locus_tag="PC523_000800"
     CDS             complement(25101..25583)
                     /locus_tag="PC523_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2474356.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKM
                     HNINVGNMSSRKDTTVLKPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            complement(25603..26412)
                     /locus_tag="PC523_000801"
     CDS             complement(25603..26412)
                     /locus_tag="PC523_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2474357.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIIVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            complement(26405..27184)
                     /locus_tag="PC523_000802"
     CDS             complement(26405..27184)
                     /locus_tag="PC523_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2474358.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQAQQYTVVGGMDEDE"
     gene            complement(27184..29262)
                     /locus_tag="PC523_000803"
     CDS             complement(27184..29262)
                     /locus_tag="PC523_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HDF2474359.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     KFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEIECPLEEIPIFLKQDSSYNFKE
                     LKNILSEVKS"
     gene            complement(29870..30037)
                     /locus_tag="PC523_000804"
     CDS             complement(29870..30037)
                     /locus_tag="PC523_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474360.1"
                     /translation="MTGYVANISQTSQALCGIRSINGIIPIVLTVAMLVQMYSLAERK
                     CREVINELRSK"
     gene            complement(30208..30729)
                     /locus_tag="PC523_000805"
     CDS             complement(30208..30729)
                     /locus_tag="PC523_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDF2474361.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAIDLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPYVRQFGEYLKNNYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCSELMDKINDIQMVEGKGIFIEELEKLLKEYNLE
                     IPIVGFDEIREGK"
     gene            30895..31461
                     /locus_tag="PC523_000806"
     CDS             30895..31461
                     /locus_tag="PC523_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2474362.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISNKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTYSHS"
     gene            complement(31732..31905)
                     /locus_tag="PC523_000807"
     CDS             complement(31732..31905)
                     /locus_tag="PC523_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HDF2474363.1"
                     /translation="MSNEKYIIKNILLKTLRKLRNNNSYGYDISEFKDMYELFIENED
                     VFVSIRKLAKYDF"
     gene            complement(31982..32851)
                     /locus_tag="PC523_000808"
     CDS             complement(31982..32851)
                     /locus_tag="PC523_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDF2474364.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRAGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDELDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIREVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIETVKC"
     gene            complement(32871..34787)
                     /locus_tag="PC523_000809"
     CDS             complement(32871..34787)
                     /locus_tag="PC523_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HDF2474365.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKADELIQRCIDG
                     KVAIRKGNSSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNATKDERKNKSAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMVGVLDKSGVLLDKNKFKEEILKREEISSTGFGMGIAIPHAKTDAVKIPRVAVGV
                     SKEGFDFESEDGSLVHIIFMIAATDNGDNLHLKTLSQLSAKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            complement(34790..35710)
                     /locus_tag="PC523_000810"
     CDS             complement(34790..35710)
                     /locus_tag="PC523_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HDF2474366.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFNVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLEKIQSLEDISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGESIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(35812..36564)
                     /locus_tag="PC523_000811"
     CDS             complement(35812..36564)
                     /locus_tag="PC523_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2474367.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINQSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(36956..37231)
                     /locus_tag="PC523_000812"
                     /pseudo
     CDS             complement(36956..37231)
                     /locus_tag="PC523_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
     gene            complement(37618..38397)
                     /locus_tag="PC523_000813"
     CDS             complement(37618..38397)
                     /locus_tag="PC523_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HDF2474368.1"
                     /translation="MHKMKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPF
                     SRQAIEMVKNDDLCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLMGK
                     EVFNYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEEVSISAMCDAAKEYGIPSCPY
                     YLDSCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSS
                     LVQERVYEHAMLCDKEVKMWLRRNNIELVDFKDIFKANNYI"
     gene            complement(38390..39817)
                     /locus_tag="PC523_000814"
     CDS             complement(38390..39817)
                     /locus_tag="PC523_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2474369.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNIST
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLAPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTPTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKRKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPIGIIFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEIQLDELRKQADA"
     gene            40208..40930
                     /locus_tag="PC523_000815"
     CDS             40208..40930
                     /locus_tag="PC523_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2474370.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNAEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKIGSEVYYLHRLRYIDNVPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            41127..42794
                     /locus_tag="PC523_000816"
     CDS             41127..42794
                     /locus_tag="PC523_000816"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDF2474371.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLSSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEELLKEAHKRDLKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDKTTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGVKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALIILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSHSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNILVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDTIENEMEIKPYESIMIYCK"
     gene            complement(42889..44208)
                     /locus_tag="PC523_000817"
     CDS             complement(42889..44208)
                     /locus_tag="PC523_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2474372.1"
                     /translation="MEKGIKLGFWSIVLLGINAIVGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGNFIGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLTKNIIIGILVVGLGIINFMGMKQSKQLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSSSSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLVGSTTPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYPKKYNNVEVK"
     gene            complement(44246..44998)
                     /locus_tag="PC523_000818"
     CDS             complement(44246..44998)
                     /locus_tag="PC523_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDF2474373.1"
                     /translation="MKKLVKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CEAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDINPLIWKEEPHNKLGAISPKRDVF
                     DMKLLKYALDMKKPVLGICRGEQIINVAEGGSLYQDLSLIEGAYIKHNQQHLSNIPTH
                     TALIKEDTKLYEILGEKEILVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTKDYDNMKKIFMAIVKEASKEK"
     gene            complement(45242..47251)
                     /locus_tag="PC523_000819"
     CDS             complement(45242..47251)
                     /locus_tag="PC523_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2474374.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGEASLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGIAMISDKGNVIICNNSLSNILDIQGNIIGKNIDELENENIKKIFRLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSLKMERTKELGMKIAKSDYTVLITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKEDRFRADLYYRINVLPVNIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     SDLPHNLLNITEDFSKEISILKDRTSLEKVKLVMGCLKNANNLNKNIGRNGIVNSINE
                     LGYSITEGEVRGILNILKELDLIICESGRRGSELNRRGKNILNSM"
     gene            complement(47687..49120)
                     /locus_tag="PC523_000820"
     CDS             complement(47687..49120)
                     /locus_tag="PC523_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2474375.1"
                     /translation="MSFKKDFLWGGATAANQCEGAWNLDGKGTSCSDMCTGGSQTVSK
                     RITRTIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEVPFAITEKYNGWASREVIDLYMNYCEAIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFISQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPNDVVKTQEDNRVMNYFCGDVQVRG
                     EYPTYMNRYFRENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSESKESNTSGNI
                     LGGVKNPYLESSEWGWQIDPKGLRYALNEIYDRYRIPMMVVENGLGAYDKKEPDGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFNWYKQVIESNGERL"
     gene            complement(49136..50560)
                     /gene="ascB"
                     /locus_tag="PC523_000821"
     CDS             complement(49136..50560)
                     /gene="ascB"
                     /locus_tag="PC523_000821"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF2474376.1"
                     /translation="MALRKDFLWGGATAANQCEGGYKEGGRGLANVDVIPQGKDRFPV
                     LTGKMKMFECDEEHYYPAHEAIDMYHNYKEDIKLFAEMGFKCYRMSIAWTRIFPTGIE
                     EVPNEEGLKFYEDLFDECLKYGIEPVVTICHFDVPMYLVKKYGAWRNRKLVDLYVKYC
                     EVIFTRFKDKIKYWLTFNEINMILHLPFIGAGLYIEEGENEEQIKYQAAHHQLVASAL
                     VTKLAHNINQEFKIGCMIAAGNTYANTCNPLDVWKSIEKDRENYFFIDVQSRGEYPAY
                     ALKELERKGINIVMEEDDKKILKENTVDFISFSYYSSRLTSADPEVNILTEGNVFESL
                     KNPYLDASEWGWQIDPLGLRITINSLYDRYQKPLFIVENGLGAVDNPDETGNINDDYR
                     IDYLRAHIKAMIDAVELDGVDLWGYTPWGCIDLVSASTGEMKKRYGFIYVDKDNDGNG
                     TLKRSKKKSFDWYKKVIESNGKEL"
     gene            complement(50647..52563)
                     /locus_tag="PC523_000822"
     CDS             complement(50647..52563)
                     /locus_tag="PC523_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2474377.1"
                     /translation="MRKYEQLSKEIIENVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSTGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSGSDSPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVFLASKLEKMFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKMKELCIPAIISGICGVTEPAIYGITL
                     PRKKPFIYSCVGGAVAGAVMGVMNVKSYVMGGLGIFGIPNYINPTTGDMTGVYATTIS
                     IIVAIVVGFVLTFFLWKDDTIVEISKDNNSKGAVEKNKREVIYSPIKGEVKALEHMQD
                     AAFSTGVLGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQTLLTFDIESISKEGYCLETPVVVTNYSDYVDIVENTQ
                     KHISNSQELITVLA"
     gene            complement(52637..53497)
                     /locus_tag="PC523_000823"
     CDS             complement(52637..53497)
                     /locus_tag="PC523_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2474378.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGDHISKDKI
                     DKIFKLSDPNICDKFKELIADIPMKYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVNVKNVLLWDIKRFYKNEFNIGLKALDYIEKKFEIRLPEDEAGFIALHI
                     VNAQMDQSIKIIYDITQIIQEIANVVKYHYRIVFDEDSVYYHRFITHLKYFAERVVSN
                     NLHENNEDDLLKVIKVKYKNAYKCIEKLDEFIHKKYNYNLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(53850..55037)
                     /locus_tag="PC523_000824"
     CDS             complement(53850..55037)
                     /locus_tag="PC523_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2474379.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMEEYGFDTIYPYCNMETAFRAELGNNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDINPMVYVHEKEFLSYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(55051..56442)
                     /locus_tag="PC523_000825"
     CDS             complement(55051..56442)
                     /locus_tag="PC523_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDF2474380.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKRFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQITPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQESMVNSDSNIKEFTKREAMVIAIFTIGMAIMIYGVLKL
                     GWYITEIAMIFTAIGIISGIASGLKQDDIVNSFISGAGDLMTAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFIIPLMVALTVL
                     VMVFLTIGLYLGF"
     gene            complement(57038..58738)
                     /locus_tag="PC523_000826"
     CDS             complement(57038..58738)
                     /locus_tag="PC523_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2474381.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVYALGLSGIIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NVNKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFLAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYREKQSVDAGKTVYNNAVGDEVDDKDTELVHSIIEGLGGSSNIKNVTNC
                     ATRLRVELNSIDGFYDDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(58710..60113)
                     /locus_tag="PC523_000827"
     CDS             complement(58710..60113)
                     /locus_tag="PC523_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2474382.1"
                     /translation="MIVNKELIIDYLKSNQENIIKDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIKIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNNKKDLVESLAYYTEIEEVEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLERIKGLNDKGVIIKDILNSYFTDDNHGKKIGIYKEDVDMGVSTICIMS
                     IFLDKNELNMKIDFRYPKGISLEFITNRINEIGKKENLIIDIYKNLKLLYVEPDSELI
                     NKLSSAYKQGFEKEAELFTKGAASYARVLENGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
BASE COUNT        19798 a        10939 c         6512 g        23476 t
ORIGIN      
        1 gtttatttca tccttatgtt ttagtttggt tagctttagg ggtttttagc aattcaagta
       61 gtattggata ggtgttatcc tatctctaca tataagtttc cctatatgct gactatttta
      121 gctactaact catgactaaa gtcacaagta tgcgtagcta ttttttaatc aaatctcgct
      181 ttattcaaat attttttaag tgattctaat atcaaatctc ctgacttaaa cactccacct
      241 gaataagata cctttatctt tgtaccttgt tcaaaataat gctctaatcc tattataagc
      301 ctacttaatt cataagctgc atcatcaaat attttactag cagtattatc accttcaata
      361 actgctttta aacatatcgt agcaaacttt gctatttcta ttctatctcc ttgtatttca
      421 tcatttacat attttattat gtcataatca ttctctatat taagatgtct ttttattaaa
      481 tcatataatt ttgtttttga acaccttcca tcagactgca ttgtgaacat ttttagggta
      541 gccattccta tataatatgc tgacccttca tctcctaata catagcccca tcctccacat
      601 ctgtgaagat tcccatcttt atctaaagcc tgtgctattg aaccagtacc tgcaattata
      661 tttatacctt tttccccatt taaagaacct gctaatgcta tttctacatc attacctaaa
      721 gtataattta tatggctata tgcatttttt gttgctactt ctaatgcata caatacttct
      781 tttatttttc catatccagc aagtccaaca aatgcatacg ttatttcttc tacatttatc
      841 ttagcatctt tacaaacttc ttccaaccca gttattaata atttcgttag attatcaaat
      901 cctatttgat tataatgaca agttcctttg gttatggttc ctactatatt taattcttca
      961 tctactaatg taaatgttgt tttcgttcct ccaccatcta cccctaaaaa atacatacta
     1021 tcctcctaca atatttttaa taattctttc aatacttaat atctaaaaat aaccttccaa
     1081 tgcagttctt ttaatattga aaggttatca gtattattat ttattttaca atctttctat
     1141 tgattttaaa gcttcactta tataaccttc ttgtctatct aggatattct ttgcactatc
     1201 cttatctaaa ccacttttta tcataaatat agccaattta acgctattat ctgcttcttc
     1261 taagaatttt tctgcaacgt catattctac atttgtagct atcattatta ttctttttgc
     1321 tctttcaacc aacttttcat ttgatgtctt aacatctacc atcaagtttg agtatacttt
     1381 tcccatctta accatagaag ctgttgtcaa catattacat accatttttt gagctgtacc
     1441 acttttcatt ctagttgaac ccattattac ttctgcccct actataatgt ttattggtaa
     1501 atccgctttt ttactcattt cactattcgg attcatagtt attgatagta ctgcacaccc
     1561 taattcctta gcatactcca ttgcacctat cacataagga gtccttcctg atgcagctat
     1621 accacatata acatccttag atgtaactcc tctatctatt atatcttgtt ttccaagttc
     1681 tttatcatct tcagcacctt ctactgcttt aaatattgct tgatttccac cagctattat
     1741 agcttgaaca agctcaaaat ccactccgta agttggagga cattctgtgg catctagtat
     1801 ccctattctc ccactggtac ctgctcctat atagaataat ctacctccct gcttcattct
     1861 atctactatt atattaacag cttttgctat actctccttt tgttcttcaa cagcataggc
     1921 tacagtttta tcttcgttat ttattaattc tattatgcct aatgtatctt ctttatctat
     1981 ttgcaatgta ttcttatttc taccttctgt aactaagttt tctaaagttt ttattaacat
     2041 atcatttcct cattttctta atattaaatt tttaaacaca ttttaattcc aaccttcatg
     2101 attatatcta cataataaat taatattttc ataataaatt attttgttga aggcttaaaa
     2161 ttaaactttt gccatggttt aatgtaatct aatataaata attcttcttt tcttatatat
     2221 cctactacat tcgatttacc actgtttttc atatcagtca aagctatttg taactccccc
     2281 gcataatgac catattcact actttcaatt actatatctc ctctttttat tattttagga
     2341 gtattaaata gttcgaattt gtatccttta tatttaacac gagattgagt agaccttatc
     2401 agattatcag atatatcccc tctattaaaa tgtatttctt caaagagtat cttcttctca
     2461 acttctcgta tattttcctc tattgctaca tcaaatgtaa ccatatcttt tctcatagaa
     2521 cctaatacct taagctcctc atatgttgga taacaatttg atattattat atcatttata
     2581 ttattcatag ctattatatg ctttacttga atatctatag gtagatttct gtgaatctca
     2641 attgttggaa gtccatcagt tataggccat ggtccaaatg tactttcatt ttgacttgtt
     2701 acaaaaactg ctgttcttaa tccatactta ttaaatcttt ctgtagattt tataaagtgt
     2761 tctaaattta atccactata tctgtgtgga taaaagttat gacatcctat caaattctct
     2821 ttgtttggtt ggtagtccat tattgtatct atataattag tattgttact catattgact
     2881 tctattttaa ggttctgagg attaaaagtc attaatgcct cttcagaccc tgtaaatccc
     2941 atatcaagcc ttattccatc tgccttcatc tttttaaaaa aagtcaaatc tttgtagctt
     3001 atttctaatt caccaaatac tctgggattt acatcaagta ttacctcaaa acctttaaac
     3061 ttagcgtatt cgtttatact tttaaatttt ttaattatat tttctttagt atctttaact
     3121 gataataaac atgaaaatat acgtgtaaat ccattttcag caaccttatc tatataatca
     3181 tatatttcct tagatgttgt tttttctgga taaattgata ttcctaatct tcccatctgt
     3241 gtcatctcct tatatcctat atgtgtttaa atttatttag aattacacga aactttttta
     3301 aaaatttggt gaaaaaaagt ttcatatata tttataaaaa aagaaaagtt taccttttct
     3361 ttttttataa acaatcaaca aaatatcttt gttacttatt tcataactgt tatactttga
     3421 aatttatact aatctaatat agtttttaat tttcaatata gtttctaatt aataaacttc
     3481 ttgctcttca actttttctt catgaagcaa cctcttatcg tacatcttga caaatggata
     3541 gtaaattatt attgatatag ctatacataa taagttgaga actgaagctc tccaatctcc
     3601 accagtggct aaatatgccc ctactggtcc tggtaatgtc caaggagcag taactactac
     3661 tctagatact aaattagcac tcatagcaaa ccacgaaact gttgccatta ctattggtgc
     3721 tacaacaaat ggtattatta atattgggtt taatactatt ggtgctccaa atattattgg
     3781 ttcatttata ttgaatattg ctggtattac agttgctttt cctaaagact ttccataagc
     3841 tgattttgct ctaaatgcaa gaagtaaagc taagcctatt gtagctccag aacctccaat
     3901 ccatataaac cattgaaaaa atggttcagc agcaatacct ggaactgctt gacctgcagc
     3961 atatgaagct gtattagcat ctaataattg tatccataaa ggtcttgcca atgaacctat
     4021 tattgataca ccatgtattc caaatgacca aaaaaacgtt attaaaaata ttagtataat
     4081 tactgatggt aatgtgtcag tggctgaaac taatggagtt actagtttag ctatgaaacc
     4141 atgccaatca aatcctagga aataagtaac aatagccatt aaaattatta ttaatgcagc
     4201 tggtgtcaga gcttcaaaag accttgtgac tgatgctggt actgcttctg gcatggttat
     4261 cttgaactta gatttgtcag taaatctata aatctcaaca cctattatag acgttacaat
     4321 agcaacaaac attgaacctc caccaagatt agccattggc atttgccatc cttgaggaat
     4381 tgaattatac caagctaaaa gttctggact cttttcaact acctttaata aatcaggggt
     4441 aagaccctct attgcagtag gaactattgt caaaaggaat gtagctgtag ctaataaacc
     4501 tcctgtcaat ccatctaact tatatgactt tgccaaacta taacctatgc caaatgtaga
     4561 atatactgat ataataaaaa atgtcatcct atatggtagt aatattgtaa ctgcattttc
     4621 ctttatatac tgagttaccc cccatgaatc tggtaatgga ggaaatgcta atatcaagaa
     4681 aaatgaacca actattataa gtggtagtga agctacgatt ccatctctta cagctcttaa
     4741 gtgacgttgt tctgccaatc tagccataga tgttgataat ttaatgtcta aaaattctgt
     4801 aaatttatct agcatctttt ctctccttaa ttaatattta tttttattat cattaaaatt
     4861 tttgtaaaat taactttctt ctactctagc aacttctaaa actgctattc actcaacagc
     4921 tcttgaactt gttttaataa ctttggccct cctaatggac tataagcttg tgcttttatt
     4981 aatgcacatg gtacaccaac ttcatcagcg aaacctttta aatagtcaaa tctatgtctg
     5041 acttgtggag ctaccatagc aatatcccat ccatttttaa cttcatcctc aaactcttga
     5101 gtgccaacag ctaaaacatc tatatctatt ccattttttg ttccttcttt ttgtaatgca
     5161 ttaactgcta tagcacttga catacctgat gaacaaacaa ataacactct catatttatt
     5221 cccccttatt tttttatttc ttttcaatca ttatagcatg tatttttaat tacaggaata
     5281 cattttcacg ctttttttca ttatttacaa aattacttgt aattttattt caaatattta
     5341 tatcttattt taatctattt atatacctaa tcattctttt tactaaaatt acacacaact
     5401 tcaaatactt atatcttctt ttacaaaaaa aagcatggag actatctcat tattaccata
     5461 aaatagtctc catacttttt taaatatgta tacctttata tatccagaag taacctatta
     5521 ttgcaaatga caaagcaaat aaaatataca gtctaaaaaa tcctaaagaa aaacctctct
     5581 tagtttcaat aaagtacctt gtatatgcaa ctgcaaaaga tgtaattaat gaatttccta
     5641 taataactcc taatctatta tctattccaa atatagataa taaaacagga aaaattacag
     5701 tacttactag agatgacact attagtatta atccactttt aaaacttata gttggaatat
     5761 tataatctgt tttttgattt ttctccattt ctctcttagt atttttatta ctttttgtaa
     5821 tttttgcatt tttataatta ttatttttct tttttttatt attagacatg tcatttacct
     5881 ccaaaaggtt taggtgttat attccctaaa ataactttac ttttagcccc tgttgcagat
     5941 attacattag aaaaagaatt atttaatgtt tcattgccca agattacaaa tgccaaagcc
     6001 tcctttgcat cagaagaata cccatattca tcctgagtta atacttcaac ccctaataat
     6061 aactctttta tatatcttat taaagttaga ttatgagctc ctccacctcc aattaataaa
     6121 gtatctacat taacctttgg aagtatgaag tttttataat tataatatat agcttttgct
     6181 gtaaacatag ttactgttgc tatcaaatca tatttatcta catgtttata tttgttaagc
     6241 atgttatcta aatagacctg tccaaatatc tctcttccag tgctttttgg tggtgattga
     6301 ctaagatatt tatgactcat caaatctttt aacatttctt cattaatttt accttttgat
     6361 gcaaagtatc catttttatc atacctttta ttaaataatt tttgacatac tccatctatt
     6421 atcatatttc ctggacctgt atcaaatgca aagacatcct ctatgttaca tgatttaggt
     6481 attatagtaa tatttcctat tccaccaata ttttgaagtg ctacattttt attgttatca
     6541 ctatataata aaaattcact atatgggact aatggagcac cttctccacc tgctgctata
     6601 tccatcactc taaaattaga tattactttt gtatttgttt catgtgctat aactgctggt
     6661 tcacctattt gaagcgttga agatacaaaa ttattataac ttcttggtat atgaaagatt
     6721 gtttgaccat gagatgcgat aaaatccaaa ttttctatat taaagtttgc tttataacat
     6781 actgatttta cagctttact aaataaatat ccaagcttaa aatttaagct acaaattaaa
     6841 tcaacactcg attcctcttc tatacagcac tttcttattt cgtcttttat atctttagga
     6901 atttctagag ttataaattc tatttgctta attttggtat ttctaccact cccactaatt
     6961 tcagctagta ctgcatcaac tccgtctaag gaagttcctg acattaaacc tacactatac
     7021 atagttatca tccttttaag tttatagtta ttcctcccca atcaaagtat taagtttaat
     7081 aattttagaa ttaatattat tccatcttga attatgataa tttactctta cgaaattaat
     7141 tgagttaatc ttatatacgg tttttacctt agctaaatct tacctcaatt tattttcagt
     7201 gtcaatgtaa tctcatgtaa ttttttttca catacaaaac acattctatc tccaaaaaac
     7261 atattaaaat agttgctaca aataaaaaac ataccaggta tctatctggt atgtttttca
     7321 tatataataa ttattatttt ataagatgga tagagcatca tttaagtcac taattaaatc
     7381 ttcactattt tcaagaacta aacttattct tattctctat ggcgatatat tttatagcct
     7441 ttataataac ctaactatct aggtttaaat aatcagatat atcataaaaa taaatattaa
     7501 aaattgtata tgaaccattt tccgaacata cctcaatctc atgccctaaa ctatctatca
     7561 ttttctttac aaaatacagc cccattccag tagacttata caattgattt cttccatttt
     7621 tccccgtaaa tcccttgtca aaaactctag atatatcttc ttcatttata cctataccaa
     7681 aatcttttat agataatacc actccatttt ctaatttttt tgaattaaat tcaatcttac
     7741 caacttcctt tgaatacttt atagcattat taataatttg ttctaaaata tatgacatgc
     7801 atttagaatc tgtataaaca tcattttcta attcattcaa actaacttct acattatttt
     7861 ttataagcaa aaatgaatta ttttttatag acattctcac cattttatct aaattaaatt
     7921 tatttacaaa taaatcttca aacatagtgt tacttctact actatacaag atagagttta
     7981 ctaagaaatt tatctttgca atctgattct taatatccaa actatcttct atgttactta
     8041 acctatctgt aattatactt aaagaagata ttggaatttt tatttcatga acccatttag
     8101 ctatgtattc atctaattct tttaaagact gtttataact ctctatgtca ctattatttt
     8161 tcatttttaa attttctata atatctttta ttagattttc ttcaaaagat tgtccatcaa
     8221 ctaaataact gtctatttca ccactatctt ctatacaatt atataaattt atataactat
     8281 ttctaaaatt tatataatct ataacaaaaa ataccattat tataaacagc actaaaaaat
     8341 ctaagtatat taactctgaa tatctatttt taaaaatatc tatagcaaat aaatagatat
     8401 ttattatact aactacaact aaaggtagag caatatactt agacctacct tttaaatatt
     8461 ttactagcat tgtattatat atcctattcc cttcttagtt tttataaaat catcaaaacc
     8521 taattcttta actttacttc tcaatctatt catatttaca gtaagtgcat tgtctgtcac
     8581 aaactcatca ttatcccata gagtcatcat aagactttcc cttgatacaa ccctattctt
     8641 attttttaat aataatgtta aaatttttat ttcgttttta gtcaactcta tacttttatc
     8701 cttatactta tgttttataa tccctttccc cacatcaaac atcaaatctt cacaatacaa
     8761 aatactatta gaatcactat aattatagac tcttcttatt ataccattta ttttggtaac
     8821 caatatgtct atagaaaatg gtttggtaat ataatcatct gctccattat ttatacccat
     8881 tattaaatcc atatcagaat cccttgatga taaaaatatt aatggtacat ttgaaatagc
     8941 tcttattttt tcgcaccaat aaaatccatt atataatggt aaatttacat ctaaaagtac
     9001 tacatctggt tttttatcca caaattcatc taatactatc tcaaaatcat caattaattc
     9061 tactttaaat ccccatttct caagtgctaa cgcaatctct tttgataaat caatatcgtc
     9121 ctctactaat aaaattctat acatttgtat attctccatt atcctaatat ttcactatgc
     9181 cacgaattat taatatcata acatatttta tataaattta ttacccaaat catttagtta
     9241 atcaacaagg agactcttat tacaagaatc tcctttctct ttattaatat ataaactaat
     9301 ttttatacac tacactctta aacttattaa cagatactgc tccatatatt atgaatatta
     9361 tcaaacatat cactaaactt agtatatata taccaaataa gtcggtcatc aataaatctc
     9421 ctgctaatct caatgcaaaa taactagata tagttgcact taccattggc aaagtataaa
     9481 atatagctac ttctttaagt atagctctat ttatatcttt ttctgtagta cctattttta
     9541 ataaaatctt gtatttttct ttatcttcat atgcatcact atatattttc atatatatta
     9601 tacttccagc tacaattgca aagactaaga ataaaaacac aagcactaca tatgcaaact
     9661 tcatccattg actctcgcct tgagctttat atccattctg aatatatcca ttttttaaac
     9721 ttaagttctt ttcaatattg ttgaacatta ctttgctatt ttcttcttga aatacctttg
     9781 caccataaaa atatacttgt tgacccttat ctcttaattt gaaatatgta tcgtcattta
     9841 ctactatgat actttgatta agtcctgtcc ctagtactgg aacttttact tctccccttg
     9901 aaatattata taactgacca tcaagtttta tcttggcttc ttttgtagct attgatgcta
     9961 tcgtttgagg gctttttaac tctattacat tatttccact aactaatctt tcaccatact
    10021 cttttaattc tttatcattg ccattcactt taagagtatt acaaaaatca ctatatttca
    10081 ttatagtatc tgttgtatca taagcttctt ttatatctgt tttctttaaa attgttagtt
    10141 ctgggtttac tgaatacttt atcttattag tatcttttat tatattctct atttgttttt
    10201 tattcacttc atctttatcc attgaaacta tgctaatagt gtacacattt ctttgttctt
    10261 gggcattctt atgtatacga ttcatcgcaa ctgaagcacc aagagttgat attgttgtag
    10321 ttataattat agctattata gcataagttt tatagttttt atttaatcgg tagtttatat
    10381 tgtttattgc aattatatta tttccattaa ataatatttt tttatttttg attataatat
    10441 caaatataac aggcataact gatttgaata agccataagt acctactatt attataccta
    10501 taataggtag tacactagtt atatctccag ttcttatatt agtagaaaga tagtagccat
    10561 atcctattac tataattgat actatagcta atatatatat acctattcct acctttggta
    10621 ttttttcttg ttttttagat atattgatta gatttattac actacttcta catattgtta
    10681 taaaaccttt tacagccatt attataaata tagaaataaa tattaaagta gcatatagta
    10741 tagcttctat gctaagtcca ttagatacct ttatattaaa tccagataac tttattatca
    10801 ctacttgaaa aaatctagat gttattatcc ctataattat tcctgttata caagaactta
    10861 ttcctataag catattttct acaaaatata tttttcctat agtatataaa tctaatccca
    10921 taaaggacaa tattccaatt tctttagacc tattcttaaa aaatatattt gttgcatacc
    10981 atataaagaa aaataaaaat actgatagta tcattattac agctactaac actgatagtg
    11041 taaatttttt attttcctct tgtaacacat ttatagcatc accatatctc aataattcaa
    11101 aattagttag cacaactact gaaaaaacca tagatataaa aaatgctaga taagttctta
    11161 tgttactctt aaaattatta taagcaattc caagtatact cacttacatc tcacctccca
    11221 tagaggctag catatccata atctttccat aaaattcttt tctactattt cctttgttta
    11281 atttacactt tatatttcca tcatttatca tatatacttc atttgaatag cttgccgtta
    11341 gagggtcatg tgtaatcatt ataatagtag cgttgttttc tctgtttatt ttttctaatg
    11401 attctaaaag ttcatatgct gatttagagt ctaaggcacc tgttggttca tcagcaaata
    11461 tgactgctgg atttgtaatt aatgacctag ctgctgcgcc tctttgtttt tgtcctcctg
    11521 ataactgata tggatattta tctaaatgat tttctaatcc aaattttttg gctatagaaa
    11581 ataccttagc ttctatctcc ttacttttct tttttcctaa tgctagagga agtgctatat
    11641 tgtccattag tttcatattg tctaatagat tatagtcttg aaatataaac cctatttttt
    11701 ctcttcttag ttttgatagt tcattatttt ttacttttga tatattaatt ccatctagta
    11761 ttatttctcc tgaagtttgt ttgtcaagtg tagacattaa atttagtaat gttgtctttc
    11821 ctgcaccaga tggacccatt atcgctataa aatcaccttc attgactgta agagatatat
    11881 tttttaacac atttgtttta aatcctttta ttccataatc ttttgagata ttttttattt
    11941 ttaatatttc tttcataata atagcctcca gttgctaatt ttttaatata tctttatttt
    12001 aaaggtttta tataaataaa tccttaagta aatattacat tttaactgtt agcattacac
    12061 ttttgtaaca tatggaagac tattatagtt atagtggaaa tatcttaaaa taaaagcatg
    12121 attcactttt ttgtataaac aaagaaaaga agcccctaag aagcctctaa tctatacaat
    12181 aattcttctc tataaaaaac caaaaataca accttgattg tggtaaatac aggtactata
    12241 aacaattgat attaccacta tatattaaaa caattattcc ttaagataat tcagtaagac
    12301 tatcaagcca taagctaagc atccaaaaag acttaatacc attaatgtat aattataacc
    12361 aatattaacg ctcaaaaaac cagcaacagt tggaataatc agagatgacc cccccataaa
    12421 tatacttaaa gctattaatt ggctactact tctaagtttt ttaggagaaa cttcaaatat
    12481 taatcttttt gaagtaatta aaattaaagg agtagaaaac acttgaaaaa cacacagtat
    12541 aataatgata tttgaatcat ttgttaaagc aaataataga aactgaactg ttatcataat
    12601 agcacttaat tttaataagt ttaaactgtt atattttttt aataaataag aaccagctaa
    12661 atatgttggt atctctaata atgactgtaa agaccacttt aaagatattt gagaatttgt
    12721 agcatttaaa gctatcattt tatctattac agtacagtta tttgctacca ccattgaata
    12781 tagtaaaaat aaaatacata ctagtaagat atatttttta tcttttaata attcaataat
    12841 atctttactg gttacattta tttttttgtt aatcttttca atatcaggta gtaaaagtat
    12901 atttataata agtaatacta caattaaacc aatagctaca gataatccaa aatacgagaa
    12961 tctaagaatc aagtaagatg ccaccatact tccaatagcc catccaattg acccaaaagt
    13021 cttaataaat gacaaataat tattaatttt atctccacaa cctattatcc atgtatcata
    13081 taaactacag caagtattta ataatcctcc tgacaaagca acaagtaata agtatattat
    13141 aaaagttgca gattcagtag aaaacataat aactgatgca attccatata ctgatagact
    13201 aattaaaaca atttttttca ttgtttgata cttatcagct aatactccaa ataataattg
    13261 aaataatata ttggttattg catagcttga taatataatt cctctttcaa atgctgagta
    13321 acctaatttt gataaaaaag gtgtgaattt tgttgatgca aaggcaaaaa taatatatga
    13381 gagaaaataa aatatgataa atctaaattt aatcgcttta ttaatcataa tcaatcaaaa
    13441 ttgataactt taataccact atttccttcc tgatgtaagt ttgaaagatg atattttacc
    13501 gtattaggat tctctataat aactaaagga atatataaag tattttcaag taatttatta
    13561 tcaatttcta ctagattttc ccccatatta acttcttgac cttgtttaac ttttaattta
    13621 aagttttctt tttttgttgt tgcactatcc agtccaatat gaataagaat ttgaacatta
    13681 ttttgtaatt tgattcctat agcatggtta gtttgagcaa taaaacttat tactccttta
    13741 gcaggtgatt taagagcatt gtcctttgct atcatagcaa atccatcccc cattacctta
    13801 gttgaaaata ctttatctgg aatttcttca agattaatta tccttccatc aatataggaa
    13861 ttaattgtaa ttacttcttt ttttctttta aagaacacta taaacactcc tctacataat
    13921 tagctttatt ctgattatca ttataaataa cgatattaag catactgcaa ctgaggaaaa
    13981 aagagcctta tagctctttc cccattgtag ttctttaata ctgcttatga tttgtttaat
    14041 tccaacaaat ttataaacat atgtaaacat gataacaata gttagtgtgt aaatttggaa
    14101 tatctgccct aataagacta aatagaaagc aatcatgttt tcttttatat ctttattctt
    14161 ttttaaatta taaataacat atgcactata tactgcacta aaattagaga ctaaatttaa
    14221 acttgcatta acatcattat ttaaaagttc tgaaaatgaa atatttgtaa ataatgatgt
    14281 caattctaaa tacactatat ataaaacagg caatattaga aaacaaccca ttataactga
    14341 aagaaatata ttttcatctt ttaatactgc tgtgacattt tttattttat ttgttatcat
    14401 gtttttattc tccttattta aaagttggcc agaaatcctt attagccaca atcatttcat
    14461 ctaagatttc ttttgctact tttgcacttg gaacagtttt actcatagta aacgactgcc
    14521 ataatttttg ataacttttt tctatccacg cttctacagc taatttttca gaagcaactt
    14581 gttgttccat tagacctttt tggaaagttg gaattttacc tatagacata ggctcaacca
    14641 tatcttttcc aacaagacaa ggaatttcaa ccattgcatc atctgcaaaa ttttcaatag
    14701 ctccattatt aggaacaatc agtaaaaatc tttcctgagt attgaatgct aatgcacaag
    14761 ctaattcaac aataaattga gaatgttcac ttgcatcaaa ccatgtatct cttgctgtac
    14821 cattttttat aattctttca cattcttcga atgtatcctt ttctcttcta tccataattt
    14881 catttgctct tgtataattt ggattggcat gttcaacagt ttcatcagcc attaaataat
    14941 attttaaata agtattaggt aatgtagttg gttctatttc taacaaatcc tttgccgctc
    15001 tcatagtttc tagccaacta gattctttat gttgaccttg aggtgtaaat tcctcatatc
    15061 cttttttagc acaatattcc tgtaatttag gcattaaatc atttccttct ttgtccttaa
    15121 tacttgtcca ccatccaaaa tgattcaatc cataatatct aacatcaaaa tctttacgag
    15181 aatttaaacc taaaactcta gcaaatattt cctctagaca tactggcata tcacaaatat
    15241 taataattct tgaatttggt cttaatacac gacatgcttc tgcaactata gctgctggat
    15301 ttgagtaatt aagcatccaa gcatttggag aatatttttc catatagtca atcatttcta
    15361 tcactggacc tattgttctt aatccatagg caactccacc tgctccacaa gtttcttgac
    15421 caacaacccc atattttagt ggaatttttt catcttgttc acgtaaagca tataaaccaa
    15481 cacgaatttg tgccattaca aaatcaatat cagtaaatgc ttcttctgga tttgttgtat
    15541 aactaaattt aatttctgga tttttctctc taacaattat tgcacatgct tcagcaactt
    15601 ttctttgtct ctcatcatta ttatcatata atttaattga ttcaatagga aatttatcaa
    15661 cactttcaat taacattgtt acaattccag cagtataagt actacctcca ccagcgatta
    15721 gtatcctctg tctttttctt tccatctttc cttctccttt acaataaatt ttcaaattct
    15781 tctcttacac ttggtactga aagtccaata ataatctgaa tagcttttcc attatgcact
    15841 aaaccatgtg cacctactgc cttaaaatct tcttctttag aaatgattga gtcatctttc
    15901 aaggttaatc tcagccttgt agcacagttt gtaacatcta caacattttc tgctccacca
    15961 actaatttta tatattcagc tgcttgttga gatgctttac ttaatttatt tcctttttca
    16021 tctttagctt ctaaatactc atttttggta tatagttttg tttcttcagt atcttcctct
    16081 ctaccaggag ttttcatatc aaacttctta attaagaaag taaatacaaa gaaccagata
    16141 aacgaaaaga taatccctac aacaacttga ataatatata ccttccagtg atacattcca
    16201 agaggaagcc aatttaatac aacccagtta ataattcctc caccaaaatc accaacaact
    16261 cctaaggcaa acgaaatcgt ttgcaagcat gcatctaaaa atgcatgtac taggaataaa
    16321 agtggtgcta taaataaaaa tgtaaattct aacggctcag taattccagt taatactgca
    16381 gtaagagtag caggaatcat taaacctaac actttcttct ttttttctgg ctttgcagtt
    16441 acatagaaag cagcaaatac tcctaatgaa ccaaacacct tcgatagccc agataatgaa
    16501 aatccaattg gatacatttc ctttagagtc ttagctgaag tttgaaagtc ctgaatatga
    16561 gtagcccaat atactgatgt accacctgga acaacaacac tatcataaca aattggtgca
    16621 tatacaaaat gatgtaatcc agttggtatt aaaatctttt gtaagaacgc ataacaccaa
    16681 acgcctatat ttccactatt tataaaaaac gtttgcaaat gtctaataac attttgaaca
    16741 tgaggccaaa taaagcaagt taataatgca actggtatca taacaaagaa acaaattgca
    16801 actacaagtg atgaacctct aaataatcca ataaaatctg gtagttcttt atcataaaat
    16861 ctattatgaa cataaataac aatacctgat attaacaaag caccaaccat accagtatct
    16921 agtgttttaa taccagcaat gcttacaagt ccacttccag cacttatttc agctgcgaaa
    16981 tcaactccaa agctttttcc ccaaaaactt aacatagaac ctacaaaata attaaaagtc
    17041 aagtatgttg ctaaagcttc catacaagct cttgcttgtt gcttttttgc aagtgctatg
    17101 ggtaaagaaa tagcaaataa taatggtaat tgattgaata ccatccaagc tccactagat
    17161 ataacacccc aaaattggta ccaaatattt tctggtgtag caagtgaacc cataattgac
    17221 tgattttgaa atacaattga gaatccaacc attatcccag caaaagcaaa taatagtgtt
    17281 ggtgtaaaca ttgcccctcc aaaacgttgt attttcttca tcatataaag tactcccctc
    17341 ctcatatttt tattttatct ttggccttag atatctatta ttttaacatg ttaataaata
    17401 tatacaatct ttggactaca ttcggagctt attcacagtc aaataagttt ttgcacattt
    17461 ttatgttcaa attttcaaaa aaaaaagaat gactacaatt tgcaatcaat cccttctgta
    17521 atcattctat gccttttata aatactatat ttaataaata acaactcaat caatataaaa
    17581 tataacattg tcaatttata actagggtgc atttgtaaaa atgagatatt agtcgctgaa
    17641 atataaattg attcatcact taaatcagtc aatgtattat ttttaaactc tgtaattgaa
    17701 attattttag ttcctattaa ttttaatctt ctagcaatat taagtgtatt ctcattctca
    17761 ccacttaggg atataataat cataacatca tttttactga caatattgta aaaagaatca
    17821 tcaaatgttc ttccctcaaa atcatatatt atttcttgac atgataagaa cattctcttt
    17881 atctgttttg caaccacatt ttgaatatca cctgaaccat aagcgtatat tgttcttgat
    17941 tcataaagaa gtttacacaa tgcatcataa tctttatttt tttgatcttc aacatactta
    18001 attattgaat tgcatgcaat atcaaatgac tgattttcta taacatgaac cttttctgat
    18061 tcccagctta ataatacttt aaattcactg aaacctttta aatctaattt tttaacaaat
    18121 cttgtaatag ttgcacgaga cacattacat ttcatagcaa gttcatttat tgacatctta
    18181 ctacaatgtt ttttatttgc agttatatat ttccatatta ataaatctgt ctcattcagt
    18241 ttattataat ttaaaaaaat catttcttct aatctcataa actattatcc cctttaatta
    18301 aaaattgtca taactattat accaatattc aacattaaat tacagcatat tcttatattt
    18361 actatttagc ctataaaatt gactataaaa aatacagcta taattgatgt taaaaaaagt
    18421 agttttaatt ctattctttt atctaataat taatattttc aaattctagt gcttttctta
    18481 tgcaattatc ctctttaaaa tcagcagtcc ttttacaatc ttttacttta aattctggag
    18541 tagttttaaa ttcctcatca catactccac tcttatttaa atacatacaa cttgccattc
    18601 taactattag accactattt tttaaattaa acaatacagg gtctatacac cctccatctc
    18661 caccagtagt ttcaccaact aaagttgcaa atttagtttc tttacaaaac attgcaaatg
    18721 actctgatga tgaatatatt tttctatcaa caagtaagta tatattaccc ttaaatttta
    18781 aatcatctgt agattttata gtataggaag tatcttcaaa accattaaaa tcttttataa
    18841 tttctttagg tgcattttcc tttacacttg ctggtaaact ttctacagga tttaatttta
    18901 tatttcttgc agttgtatat ttttttacta tttcattatt atttctataa attctataac
    18961 catttatttt gacatcattt tttatgagtt tagatacgat tccttgccaa tacctatcac
    19021 ttccacctaa attacctctt atatcaataa ttaaagcttt atgttttttt aaagtgctaa
    19081 tataatctcc aattattttt aaatcattat ttatagaccc attctttgat gacattgatg
    19141 gtaagtacat atatccaatc ttacctttaa taacatcctt taaaatcaat tcttttttta
    19201 caaaaatatc atctggcatt tttattttag gattcataga gtcatatcta tctacaactt
    19261 ctttatcatt aagaaaatca taccaatcat tattagagta tgatttctta aaaagttcat
    19321 atcttttctt gttatctatt acttctgtgt gcttattatt taaatcagat aatatagatg
    19381 acagttcttc tataaattct tcgtcatttt cagtacttct tattcttttt atatattcat
    19441 ccttattttc taaccaatca atattattaa gcctcttatt tacacctaga tatggatatc
    19501 ctgtttttac aacattatac acataattaa agtcatctac cttttcttct tcagacaact
    19561 gattcttttg agaataagta gaattaccta ataagattat aaatgctaaa ataattacta
    19621 aaaataatac tttctttttc atgtaattct catccacttt cagattttat attttaatac
    19681 attgtatcat tattttatta tcacctaaac aactaaaaga aattcttgaa gatatattcc
    19741 actattattc tctttaaaat atttttattt atatttttaa aattatttca tgaaaattat
    19801 aaaataacaa tcatattcaa atatctgttt tacttccata tcctgaaagt aaattcttat
    19861 cagtgatata ctaaaaaaga tgtgctaaaa aatttatcca ttataagggc ttaaatttag
    19921 atactttact gatttttatt tattagttga gaaagatatt gataattaaa aatattgata
    19981 actatagaaa tgattatttt ttatataaat ttaaggatat aaactaatta tacttaagtc
    20041 tatatcctta atttatcatg attacgaaaa tttttacaaa cacttatatt tgtctgtttt
    20101 agaataaaaa caaggacata tcctgttatt ttattcaaac atccaagttt aattactaca
    20161 caattacttg ttagaaattg tatctacttc atatatatat ctgttaagta tagcttctag
    20221 catttcttgt cttccagact tattctttat aactggcatt tgcagtgcat aagattctaa
    20281 ctctttaaat ccaaccttac cttctactat atctttacct ataccctcac taaaacttgc
    20341 atatctttct ttagtaaagt tttcaaatac ctcatcttct aataatttat gagctattaa
    20401 caatcccttt gcaaatgtat ccattcctgc aatataagct aggaataagt cttctacctc
    20461 aaaagatgct cttcttactt tagagtcaaa attaaatcct cctggtgcta ttccaccttg
    20521 ctttaagact tcatacatag caagtgttgc atcatatata tttgttggaa attggtctgt
    20581 atcccaacct aataataggt ctccttgatt tgcatctata ctacctaaaa cattgtttat
    20641 tctcgctata tttaattcat gttggaaagt atgccctgca agtgttgcat gatttgcttc
    20701 tatattcact ttgaagtatt tatccagatt atactttctt aaaaatccta aaacagtagc
    20761 agtatcaaaa tcatattgat gtttagtagg ctcttttggc ttaggctcta ttaagaattg
    20821 tccagtaaat cctatttcct tagcataatc tacagccatt tgtaagaatc ttgcaaagtt
    20881 atccatttca agttctgtat tagtgttaag taaagtttca taaccttctc ttccacccca
    20941 gaatacaaaa ttttctcctc ctaagtactt agttatttcc atagcttttt taacctgagc
    21001 tgctgcatat gcatatacat ctgcattaca tgttgtagat gcaccatgaa caaatcttgg
    21061 atttccaaac atattggcag ttccccataa taactttata cctgtctttt ccatattagc
    21121 ttttatatac tcaactattt catctagtat cttatttgtc tcttctaaat ctcttccttc
    21181 tggagctata tctctgtcat gaaaacagaa atactcaatt cccatcttag acataaattc
    21241 aaatcctgca tcaactttaa tctttgctat ctcaattggg tctgttatat tattccaagg
    21301 tctctctact gtacctactc caaatgggtc attaccctct ccacataaag tatgccaata
    21361 gctcattgca aatcttaagt gttctttcat agtcttattc cctactactt gttcaggatt
    21421 gtagtatcta aatgctaact cattgttaga ttttactcct tcaaatttta tctgtcctat
    21481 tcctttaaat atttcattca tttcatgtcc tcctaatatt ttacacatat aatatatgta
    21541 gtatgttttc ttaatttaaa actattttct atctttattt tctttttatt ttaagctatc
    21601 ttgtctattt atttttgcaa ttaactgctt catctaacaa cctaaagtcg tctttcaaag
    21661 atttgtatat ttttttatac aatctgtgat atgtcttgta tatatctaca ttttcactta
    21721 ttggttctaa tttagttgaa gtttttatta tagtatcaca agctttttgt atattttcat
    21781 atattccagc tcctactaat gcaagaattg caactccaag tgctggtcct tcacttgaat
    21841 ttatagtata tacatcttga ttaaacatat ctgcttgcat ctgtctccat actttacttt
    21901 ttccacctcc acctgaggct cttacttcat taacgtctat attaagattt tctattatat
    21961 ccatacagtc tttaagacta tacgaaacac cttccattat tgaccttgcg atttcttttt
    22021 tagaatggat tggagacaat ccaaagaaca cgcctcttgc ataagggtca agatgaggag
    22081 ttctttctcc catcatatat ggtaagtaaa gcaatccatt acatcctgtt ggtacttgac
    22141 ttacttgatt atttataata tcatacacat cttcgtctaa agaatttgaa acttccactt
    22201 cactttgaca gaaattatct ttaaaccatt ttaatgacaa tccagctcct tgagtaactc
    22261 ccataacatg ccatgtattt ggtattgcat gacagaaagt atgaactcta ccttctttat
    22321 caatttttgg ctcttttgta taagcaaata ctacacctga tgtacctata gtggacgaaa
    22381 ctactccatc tttaactatt ccatttccta tagctcctgc tgcttggtct ccagctcctc
    22441 ctaccactat tgttccttct ttaagccctg tcaaacttgc tacatcttta gtcacataac
    22501 ctgtaacctc ttgtgattca taaactttac ctaaaagatt tttatctata tttaactttt
    22561 ctagtacttc atcgctccaa tctctttttc ttatatcgag caactgcata ccacttgcat
    22621 ctgatacttc tgtagcatat tcacctgtca atttgtatct tatataatct tttggaagaa
    22681 gtattttatt agtattttta tatatatctg gttcattgtt tctaacccat aatatctttg
    22741 atgctgtaaa tcctgtaagt gctggattag cagttatttc aatcagtctt tcttcaccta
    22801 ctaaatcagt tatctcctca cactctttta ctgttcgttg gtcacaccat attatagatt
    22861 ttctcaatgt tttgccttca ctgtccagca taactagacc atgcatctgt cctgataatc
    22921 caattccttt tatacattca ctacttatat ttgaactttg aactacttct cttatagatt
    22981 cgactacagc tttccaccaa tcatttgcat cctgttccgc ccaacctgat ttttcttgta
    23041 taagaggata ttcatacgta caacttttta tagtatttcc tagtttatca aataaaactg
    23101 ttttagtacc tgatgtacca atatccacac ctaatacata ctccaacttc atccctcctt
    23161 gaatgccact tcataattat cgttttccta tcctaatttt tttatatttt aggtatattt
    23221 tacttgcttt atttttgtta gtatattaaa ctaacttatg aactaataat aaaatattta
    23281 attatctttg tcaatgcaaa ataacctttt atttttgtgt aaaaagtatt acaatattat
    23341 tttaaggagg aatatcaaat tggttactga taaatacact attcgagaaa tgaacgaaag
    23401 attagtactt gaacaaatta taaaaaatgg tcctatttct agggcaagta tagcttctac
    23461 tataggattg aataaagcaa ctatatctgc tattaccaaa aaacttattg atgaatctct
    23521 agttcatgaa ataggaattg gaaatagtac tcatagtggc ggaagaaaac ccatattatt
    23581 agtatttaat aaatgtgcag ggatttcttt aagtatggat attggatacg actatatatt
    23641 ttcttctatg tcttacttag atggaactat tataaattct aagaaattaa cagatataca
    23701 agtaagtaaa gacaatgtta tacaattgat agatgaaatt ataaatagtt ataacatatc
    23761 caaaatagac actccttata aggtaatcgg tctcactttg gctatacatg ggattacttg
    23821 tgaaaataaa gtattattta ctccatatta caatcttaat gagattgact tatatagcat
    23881 ccttagcaaa aaatatgact ttcctattca tatagaaaat gaagcaaact taacagcttt
    23941 agccgagaat actttttcaa ctgtacatga ctcactactc agtctcagta ttcacagtgg
    24001 atttggttca ggaattataa taaataataa attatatagt ggcagaaatg gtatgagtgg
    24061 agaaattggt catactatca tcatgcctaa tggtaaacta tgtccttgtg gaaaccgtgg
    24121 ctgtttagaa cagtattgtt cagaaaaaaa ggtatttgaa cagctttctt ctttagaaaa
    24181 tattcctaag atagattctg atatagtaaa gaaactatat tatgaggata accagaatac
    24241 caaaaaagtt atccatgaat tttgtagcta cttaacaata gctataaata atgctattac
    24301 aacctatgct cctgagataa tatatttaaa tagccaaata attagtgata tccctgagat
    24361 attacagatt acaaaagata tgttagttag ctcttttaac aaaggaataa atatagaaat
    24421 ttcatcatta gggtctgagg catctctata tggtggctct gctgtaaata tcaagagctt
    24481 tttgaatata caaaatttaa ctttaattaa tgaaatataa gggttatgac tatatacagc
    24541 cataaccctt taaattatac attctaaaat taaatttatt acatagatta cgcaagatta
    24601 gctatatctt ttaaaaattg tatatcaatt aagcattaac ataagtattt tgttttaaat
    24661 attaatataa tcttcctact catcatcaga taataaactt gtattttgtt caaccataga
    24721 aatattatct ttacctattt tctctaattt tactaccaat tcatttgtat ctaaaaaagc
    24781 tgattcaata accataccta aatttagccc tgaaataatt ttaattcgac tatcattatt
    24841 ggctactaat aaagcagcat tgcatggagt tccacctagt aaatctgcca ttataattat
    24901 ttctgaactt tccttgtaca cttctaaagc tttttttaat ttttctattg ttccttctat
    24961 tccatcatct tgtttcatat ttacagtagg ataatctata gaatccccta ttatcatttt
    25021 tgcagaattt aatacttctt cagccatatt accatgactc atcaaaataa tactattcac
    25081 tttaatacac ctctttaaat ctataagatg tctacaagat aagttttctt atcatcaggc
    25141 gtcattatag ttgtaatctc aattccttca tctaaaagtc ttttaaatgc ttctttctct
    25201 tcttcagtaa cactgatatt aggttttagc actgttgtat cttttctact tgacatatta
    25261 cctacattta tattatgcat tttaaatcct gcctctacaa atggaataag gtccacagga
    25321 gacttaacaa ttagtaatac attttgccct tcatactttc ctgcagtaat attttttata
    25381 gcagtctctt ctgtaattat agatgacgct atacctgcag gtgtagccat tcttaaaact
    25441 tgtttttgaa cagaattgtt agctactgca tcatttacta ccattaatct tgtaacacct
    25501 aatttattac tccacatagt tgcaacttga ccatggatta aacggtcatc tatacgaata
    25561 tttttaatac ctttcatttc catatctaat accatccttt ccttatttta aaatacctaa
    25621 tgcatataaa attattgaaa caaccattac tcctaaaatt aagtatatag gttttatatt
    25681 ctttttaaga gcccaataaa caatcacaac taaaattgca ggaactaacc caggcataat
    25741 ttggtctaat atttcttgac ctttcattgt aaaatcacct tgttgaaagt tcaaagcaac
    25801 attagctttt actactgaag gtattaaggc acctacaacc attaatccta aaatagatgt
    25861 gctctcagtt aaaatcttta aacttttttc aattgaagat attagcttag tacctgatgt
    25921 ataaccagca ttaaatagcg gtatacttaa taatttcaat gctacactca ctataatcca
    25981 cagtaagatt ccaaatggac ttccatctat cgccatatta gctgctatag ctccaaaaat
    26041 tgtccacgga ataacaacaa ataaactatc tcctaaacct gctactggtc ccatcaagcc
    26101 tgttttaatt gaagcaacag cttcttcagt ctcttcactt gataattcct ccattgctac
    26161 attaataccc aaaatataag gtgccaccca aggattagta ttaaaaaact gtaaatgatt
    26221 taatacagcg ctttgtaatt tttctggatt atctttgtat actactttta aaaaaggtag
    26281 tactgcataa cagtatgcca aggcctgcat cttctcataa tttgaagaaa cagaactagt
    26341 aaaaaaccag cgccataaga cacttttttt cactttttca tttgatttat ttaatgatac
    26401 cttatcactc atcttcgtcc atacctccta ctacagtata ttgttgtgct tgattttcca
    26461 aattcttttt gtaaattata agtgcaattg ctccaccaat taaagctaca cctaataatg
    26521 gtactttcat atatactgct aaaacaaaac caattactaa ataactaaaa tactccttag
    26581 caggaagata tctaagtaat aatcctatcc ctactgctgg aagtagtcct ccagctactg
    26641 ttaaaccacc tgttaaccat tcaggcgtat attttaaaat tgtattaatt aaagaaggtc
    26701 caaatattac agatagagca actggaatac ctgttgtagc tgctgttaat aacactccta
    26761 acatctgcat atatgttata tttttataat ttcctttttt agctccatct tctgctttat
    26821 gcactaacca tataccgata gtatttctaa gaacatctaa ttgtaccatc aataaagcaa
    26881 ctggaatacc tatagaaatt cctacagatg cttcttgctt agttgtaata gttataaatg
    26941 tggctactat actggctgtc tgataatcag gtattgatgc tccaccaaaa ttactaatac
    27001 ctaatgataa tagttgtaat gtacctccaa tatataaccc tgtatttata tctcctaaga
    27061 taagaccagt aataaaacct gctgttactg gttgatatgt tccaaacatt gttgaatttt
    27121 tttcatagtt tataaaaaat ccatatagta cgattaataa taattgccat aattgtggct
    27181 ccattatgat ttcacctcac tcaaaatatt ttttaattct ttaaagttat aactagaatc
    27241 ttgctttaag aaaataggaa tctcttccag tgggcattca atttcataag ttttcccacc
    27301 ttcataacta actccactaa atatattttc ccatgtatgt ccttttggta aatatacttt
    27361 tctacttcta tctttataat tcattattgg agctaccaaa atttcatctc caagcatata
    27421 tgtattatcc acctcccaag ttttatcatc ttcaggaaat tcataaaata gagttctcat
    27481 aactggtgtt ccatcttcat gtgcttcttt catcagtttt tttaaataag tttttaaact
    27541 ctctctaatt tttataaatt tcgtaagaat tacttcaact tcttcaccat aagaccatat
    27601 ttcattagga gctccagtta ccattgagcc tccaccttta tttgacaatg gtttactatg
    27661 aggcaatcta tctccatgca ttctcaaaat tggtgaaaaa gtagcatatt ggaaccatct
    27721 taccataagt tctctaaact ctgggtcttt aggatttcct ccatggaaac ctccaatatc
    27781 agtagtccac catggtatac ctgccagtcc catatttaac cctgtattta cttgattatt
    27841 gaaagcctca aaactagagt caatgtcacc agaccatact aaagctccat acttttgagc
    27901 tcctgcccaa gctcctctca caagagttac aacagactct atgtcattat gtaagccatc
    27961 ataaatcatt tttaaataat caataggata aatattacca caacttaaaa cattaccttt
    28021 tttatatcta tagttatcaa aatcatacac agaatagcca ggttcagcta catctaacca
    28081 ataataatca attccaagtt gtttataatt tttatctatt ctatcccaaa catattctct
    28141 tgcattttca tttgtcatat ctacaaagac tgtatttcct tgaatttgca ttgtcattct
    28201 aacaccacga ttaacattga ctaaataacc attttctaaa taatcattgt agttttctgc
    28261 atcactttga actgtaggcc atacagatac tattggctct actgaatact cttctttcaa
    28321 ttttttaact aactctttag gatttttcca ataatctaaa tcaaatttat attcaccttg
    28381 cttaggccaa tgaaaatagt caattgcaat tgatgaaagc ttaattcctc ttttagaata
    28441 ttccttcaca acatctaaaa cttcttctga agttctatat cttagtttac tttgccatag
    28501 tcctaaaagg ttttcaggca tcataggaac agttcctgta acttgagaat agtttttatt
    28561 caactctttt gggctttctc cacaagtaat ccaataatct ataaattttg tagaaaacat
    28621 cttccattct gtcatatttt tagcaaaaga tactttccca atacccggat tgttccataa
    28681 aagtccataa cctagactag aaatataaaa aggaactgat atttgagaat ttctttgggc
    28741 taactccaaa acagtattct ttaaatctaa aaatttatgt tgatattgtc ccattccaaa
    28801 aatcttttca gaaggctcag attcaaatct agtagttact tcaaattctc cactatagct
    28861 tggtcgatat tctcttgatt ttaactttag agtagagtta aaatctttgg taatttcaat
    28921 tgtccctaca tcttctccac catcatcatg ttttacagct cttaatcgaa tatattcttt
    28981 caataatatt tcatttttat cattgtaaaa agtaattcta tctctatggt ctaagacagc
    29041 ttttatcttt ccatttttaa tgattgcact tccatcttca tttttattaa ttgtaatatc
    29101 atctttattt aacttaggtt tttcgttaag agcatagttt tcatcaacaa agttttggtc
    29161 tacaaacgac ctgatacgta aagagttttt tccccatgct tctattcgca atagttcatt
    29221 gtcaaatctt ttttcaatat agtcttcaga tatttttatc acggctacct cctaaatcaa
    29281 tggtataata ttctatcttt gtgtagctta agttaaataa tgtcatttag tttcgttatt
    29341 tataaattag attttatata attcatttgt acattaaact aacttaccaa taatatactt
    29401 tatttgactt tgtttgtcaa tcgctttccg gattttagta aaattttcta atatattgat
    29461 ggaaagtcat ccttaatatt ttttatttat actttttaat atctgtgatg atacctatat
    29521 acatctagat ttttaaaaca catacaggaa taatgtttac ctcttatgta aacaatactg
    29581 gacttaaaaa tatgtatagc gaccttataa atacagatga taatatatta gatattattc
    29641 aaaacaaagg attttataat tataaataat ttatgaaatt atttaagaaa atatataatt
    29701 gtacaccaag tcaaaaacgg caataaataa ataaaactaa aaaatacgtt gtctcaaaat
    29761 atagataaat ttatttttca tttctgaaat gattaaaata tatttgattt aatataaaaa
    29821 agagtgtctc atgaactaaa aagttcactt ttagacactc ctttatatat tatttacttc
    29881 ttaattcatt tattacctct ctacattttc tttcagctag tgaatacatc tgtactaaca
    29941 tagctactgt aagtactatt ggtataatac catttataga acgaatacca cacaaagctt
    30001 gtgaagtttg agaaatattt gcaacatatc ctgtcataga tagtaccaaa taccaatatg
    30061 aatattaatg ataatattga tttttttaaa tttttcttta ggagtactaa ttcctccact
    30121 taaaaatgct tcatgtactg tccatcagtc atatttttct accaaatatg atgttgtttc
    30181 ataatgagac aataaatatt tatacattta cttaccttct ctaatctcat caaatccaac
    30241 tataggtatc tctaaattat actctttaag tagcttttct agctcttcta taaaaatacc
    30301 tttcccttcc accatttgta tatcatttat tttatccatc aattcactac aaccacttaa
    30361 ctcaccaaac caacgtttag aactacatgt cttattatat ccacaacttg ggctaccatc
    30421 tattgcaata atacttttta tatcataatt attttttaaa tattcaccaa attgtcttac
    30481 ataaggtatt aacatttgcc tacattgttc tctaaaaaac aaattgtcaa actgttcttt
    30541 tacatgtccc cacctattta ttccatacat aatcatttct ggacatggta gttgaattat
    30601 tccataccct ttatttatta aatctattgc tatattttgt gctcctttaa attctgacaa
    30661 cccctctact ttagaattac aatttaatat acagtgacat actactacaa tctctctatt
    30721 tctcttcata ataatctcct tagttattct taattttaat gatactacat attttgataa
    30781 ttatattatt tatttgttct atatttccaa tattctatat agaaatatac aattagtaat
    30841 cttttataat ctatactatg ataaatgtat atattttttt aggaggtatt ataattggat
    30901 actaaaaaag tcacattttc ttcaatgtgt atagttatta atattgtctt aggtatggtt
    30961 atatctatga taaatatacc attattattt ttagatacag ttggtactgt acttggggct
    31021 gtcgtattag gtcctttttg gggtgctctt attggtgggt gtacaaactt agttttaggt
    31081 gttatatcag gacctacaaa tatacctttt gctcttgtaa atattgttct tggtcttatt
    31141 gttggatata tttctaataa aaaaggtttt ggttataaag aagctattat tacaggcgta
    31201 attttatcta ttgtttgtcc tttaattggt actcctattt cagttttatt gtttggtggt
    31261 ttaagtggaa gtggtgctga tttattggtt ggttttttag ttcagtctgg tcagaagata
    31321 tttactgctg cgttctttcc aaggattctt agtaatatag tggacaagcc tttgtcttgt
    31381 attatggtgg tatattttat aagtaaaatg cctaaaggtt ttataaaaca atatagtaaa
    31441 aatacgtata gtcattctta gtaaactgta tatattatat gatttgctaa aggttataat
    31501 gagtttatac acatttattt gtataatata ttttgcactt tattattcat taattttagt
    31561 attggtgatt taattatgtg ttatctttat taatttaatt aaatacaacc tcttaatata
    31621 aatttaggtt ctaaaactgt attaagaggt tatattttta ttttataata tatttaaagt
    31681 tttatgagaa cttatagaat atacattttt tattttagtt aaaatctctc tctaaaaatc
    31741 atattttgca agttttctta tactaacaaa tacatcctca ttttctataa acaactcata
    31801 catatcttta aactctgata tatcatatcc ataagagtta ttatttctta attttctaag
    31861 cgtttttaat aatatattct ttattatata tttctcattt gacatcttaa atatacaaaa
    31921 taaagtaaga tatctactat agatatctta ctttattttc atttataatt atattttatt
    31981 attaacactt tactgtctct attgcttttc ctacacatcc acaaacttcc attctactct
    32041 taacaaaatc tttaactgac tctaaacctt ttttaccata tttcttaggg tcaattacct
    32101 ctgggtcaga attaagcaca tccttaaccc cattactata agcaatcctt aattctgttg
    32161 cataattaac cttgcaaatc cctcttttta tagattcctt aataacctca tctggtattc
    32221 cagacccacc atgtaatact aatggaactg atacaacctc tctaatttca gataatctat
    32281 ctaaatcaag ctttggctct cccttatata gcccatgtgc cgtacctatt gctactgcca
    32341 aagatgatac ccctgttctc tctacaaact cttttgcttc taatgggtct gtatatgctc
    32401 caccatcacc accatctaat tcgtcttcct tacctccaac tttacctaat tcagcttcaa
    32461 caggtatatc tgaaggcata catgcatcta caacagattt agatattgaa atattatctt
    32521 cgtaactttc atgagaacca tcaatcataa tagaagtata tccagctcta agtgcctgca
    32581 tagctagtcc aaagcttgaa ccatggtcta agtgcattgc tactggaaca cttgccttct
    32641 ctgatgctac ctttacattc gctaaataat aatctagtcc tgcatacttt attgttgaag
    32701 gtgttgtttg caatattact ggtgatttaa gctcttcagc agcctctatt actgccataa
    32761 ccatctccat attttcaaca ttaaatgcac ctactgcata atgcccttct tgtgcatcta
    32821 ataatatttg tttagttgta actaatgcca taatctctcc tcctatatgt ttatactgat
    32881 ttgatttctt cgttatttaa ttttgatact atttccctac ttgtcttgct atttaataac
    32941 tcatttaaaa attcttcatt cattaatttt gcagataatt gagataatgt ttttaagtgt
    33001 aaattatcac cattgtctgt agctgctatc ataaatataa tatgtaccaa gcttccatct
    33061 tctgattcaa agtcaaaccc ttcttttgat acacccactg ctacacgtgg tatttttact
    33121 gcatctgttt ttgcatgtgg tatcgctatc cccataccaa aacctgtaga tgatatttct
    33181 tctctcttaa ggatttcttc tttaaactta tttttatcta aaagaacacc agatttatca
    33241 agtactccaa ccatttcatc aattacatct gttttatttt ttcccttaac atctaaaatt
    33301 actgtttctt cgctatataa agagatatta ttagatacgt tttccgattc tgcactttta
    33361 tttttacgtt catctttagt tgcatttgta ttttctatag tatttttctc atttactact
    33421 ttaggtgtaa ccatcatata aagtacagca cctactaatg aacctacaaa tatacaccct
    33481 acccataaaa gtggcttatt gactaatcct agaaccaaaa accctccatg tggagctggt
    33541 acttctattt tcattatata agttaaaatt gatgatattg atgatgcaat cataagtact
    33601 ggcaacactt ttaatggatt ttttgctgca aaaggtattg ccccctctgt tatatgagta
    33661 ctacctagta tataattgat taatcctgcg gctctatctt cttctgtaaa tctgttttta
    33721 aataaagtgg atgctattgc tattaccaat ggtggtgtta tacaagctgc agatacacct
    33781 gccatgaata aataatttcc ctgtcctaga agcatagttc cagttacata tgctgcttta
    33841 tttactggac ctcccatatc aaatgctgac atacatccta ttactattcc caagattata
    33901 gggcttgaat tttgcaaccc tgatagaaag ttcatcatag cattgttaag tgctgagcat
    33961 ggtccaccaa gtaacaacat ggctacaccc acaagaccta cacttacaat aggaattata
    34021 aaaatagatt taagaccttc taattgtctt ggtaattttc ttaacaaatg cacaaataat
    34081 ttaactaagt aacctgctaa aaatcctgat atgattccac ctaaaaaacc cgaacctgta
    34141 tcgaatgata tcattcctcc aacaagtcct gccaacatac cagctcttcc cgatatagat
    34201 tgagctataa atgctgccaa cacaggaacc atcaaattca tagaatatcc accaacagat
    34261 tttaatgtag ctgctatttg attatattgt tctgaatttg ggtcaaatga ataaatcccc
    34321 cataaaaatg atatagccgt caatactcca cctgctacta ctaaaggtaa catgtgagat
    34381 acaccattca ttaagtcttt atataacttt tgaccaaacg ataaatttcc ttcttctgca
    34441 attttactgc ttgatgagtt tccttttcta atagctactt ttccatcaat acacctttgt
    34501 ataagttcat cagctttatg tataccttct ttgacaggta cttctattac tggcttccca
    34561 ttgaatctgt ccatatcaac atttttatca caagcaacta ttattgcatc tgccatttcg
    34621 atatcttttg ctgttaactt attttctaca cctatggctc cattcgtttc aacttttata
    34681 tcacacccta attccctagc tgaatttttt aatgcttctt ctgccataaa agtatgagct
    34741 attccagttg gacaacctgt tacccctaga atcattttct ttcccatttt tataccacct
    34801 ttctgactct tatatttttt atataatctt ccacattctc caaattacct ataccattac
    34861 tttcagccac acttgctcct gttgctgctg acctttttaa tgcttcttca atatgttctg
    34921 gattttccaa ccatatactt aaaaaagcag ctaatgcaga atcaccagca catgcagagc
    34981 tgactacggt aactggttgg gcactagcat aataaattga ttctccatta taaaaataag
    35041 aacctttttc tcctaaagtt agtaatatat tttgagcgcc ttttaaatgc agtaatttaa
    35101 gtacatcctt aatatcttct tcatctctta caataattcc aaatatatct ttgatttctt
    35161 catcatttgg ttttatcaaa tatggattat actgtagtaa ctcctttaat tttggtgaac
    35221 ttatatcgag tattgtcttt atacttttat ttttacaaat cttaaatata tcctcataat
    35281 aattatcatc aataccagga ggcagacttc cacttattga taaataactt atatcttcta
    35341 atgactgtat cttctctagc atatctaatt tttgtttttc gtttacatac gacccagagt
    35401 ttacaaattt aaattcatca cttccatcat ttaaaaatat atttatacga gttgtatctt
    35461 ctacccaagt aggcaataca ttaaatcctc tcttttcact ttcttcaact atatattttc
    35521 ctgaaaaacc tccaaaaaat cctaatatct tagattctat accataatga cctagtacaa
    35581 atgttacatt taatcccttt ccattaggag aatatacagc atttgatgta cgatttacta
    35641 attttctctt tatcccatta gtagatatat tcatatctat tgcaggattc gtagttaatg
    35701 tatatatcat tgtcttcccc cttgcttgtg ctgtgtatgt tttcattata ataaaaaagt
    35761 ctatgaaaat cttttcacaa atagaaaatc gattttcata gacttttcaa tttaattttc
    35821 agaattatta tatctgacca tataatctat gattatccta gatataatat acaagggcaa
    35881 tctagaggat acttcatatt cagaaattga tatatgttca tgtttgtatc ctacaatata
    35941 taaatctgaa tactttataa gttttgcact ttcactacaa caacaagtta taactttagc
    36001 tccaactaaa ttggcattct gtgctgattc tacaagctct atggtatttc cattcaatga
    36061 aaatataatt ataagttcgt ctttttttat tgatttagat tttattttca ttatgttggg
    36121 gtcatttata aaaacaacat taaaatcaag taattgcagt ttaagactaa actcttttgc
    36181 tacatattca gtaagccctc ttgctaaaat atatatctta ctagattgat ttatagtttt
    36241 tattatatta attagtgtag ttaaagatat ttgttctata actctctgtg cttctataaa
    36301 ggatttattt attatatctc ccatattttg tatatcatca ttttcatctt ttactgttaa
    36361 tctataccta agttcattga atccatttaa accacatttt ctaattgctc ttgaaacagt
    36421 tgctggagat gaatatgttt catgagcaat ctctactata gataattctg gtaatattga
    36481 ttcattttca tttataaatt taattatttc aagttccgtc ttcgttagat tttcagaatt
    36541 acttttgttt agctcaatta tcatatgtta tctcccctta tataagtttt atttgtctaa
    36601 atttaccgag taatataata tattacctct taactattta tttttatata caattaagat
    36661 ttagtcaatg tctatatatg taaatttata atatcataat tttttactta aagtttatta
    36721 tgttatttta tagcatttta agataaaaaa gaattgtacc gctactaaaa tccggctgaa
    36781 atacaataaa tattcctcat cctgatatgt ttataattaa aggtataaat ataaactttg
    36841 ctatgaatca cataattaat aaatcatttt atttacaatt ataacccccc ttataaaact
    36901 atattaataa tatatacagt atttataaag gaggttattt atatttaaaa actatttact
    36961 tttctcttaa ttcttctcca ttagttctta ttacattttg ataccaataa aaactatctt
    37021 ttttataacg ttctttgttt ccatttccat tattatctat atccacatat ataaatccat
    37081 atcttttttc catttcctga gatgaacaac ttactatatc tattggtgac cacatacaat
    37141 aagctaatat ttcagttcca tcagctatag cttcacctat ttgctctata tgttttgata
    37201 aataatctat cctgtaacta tcatgaactt ttcagatact cataatctcg gttttgataa
    37261 taaagtatac atagttatta ctactttacc aattagtgat agatatccta taccctttgt
    37321 ttatgtaaat ccttacctaa acggaaagaa ttatgacaat atccaaatta aatttcttca
    37381 tattataagg tagaaaaaat atacttattg tacaaaacca tttagaaatg tactttagtg
    37441 aatctttatt tatgaaaaat atctatctct ttttattata cataaaaaat gtgtacttcc
    37501 tatattgata gggatgattc aaatttttat tttgatacat ctctatcaat attcttttag
    37561 aaaatacaca tttttttaaa tacttatttt taattaaaac tttaagtaat ctattcttta
    37621 tatataatta ttagccttaa aaatatcttt aaaatcaacc aattcaatat tatttcttct
    37681 taaccacatt ttaacttcct tatcacatag cattgcatgt tcatatactc tttcttgaac
    37741 aagagaagat gattttataa gactataatc tataaatcct ggatgagtaa ccatcaatgt
    37801 tatatcttta tctaaattta tatacttttc ttcaaataat ttaacaaaag atttatttaa
    37861 cttataaaaa tcattctgag gatatttact tggcatacta atacatgaat ctaaataata
    37921 gggacaactt ggtataccat attctttagc agcatcacac attgctgata ttgatacctc
    37981 ttcttctata ccatgtatat caatatactc aggcaactta ctcatataca acttgtaagt
    38041 ttcaatttga gcacaaactt cttcatatac ttcttcataa ttaaaaacct ctttacccat
    38101 cagtaactgc gttcttctta ttttgctaga aacaaatttt ccaccttcat ctaacaaatt
    38161 ctttatctta atatgttctt tgcaacaagg ttctcctatt actaagttaa catgtaaacc
    38221 aaaacataaa tcatcatttt ttaccatttc tatggcttgt cttgaaaaag gcatattcac
    38281 catgattccg gcacttgtaa caataccatt cttataagaa gatattatac cgagtgtaac
    38341 accttctgaa tatcctatat catctgccct aactactaat ttcttcattt tatgcatctg
    38401 cctgctttct caactcatca agttgtattt ccatctcatc ctttacttta acaacatccg
    38461 ttcctatgat tatttgaact gatgtattac tcaatttaat taccccttta gctcctgttt
    38521 ttttaatatt atcatcattc aaaatagtgg catctttcac ttctaaacgc aaccttgtca
    38581 tacaattatt aagagtcact atattttcat aaccaccaca atttttaatt atctgcttag
    38641 ccatatatga aaaattacca tgagaaagtt ttaaattctt ttctttttca ctaactttat
    38701 cacctatctc tatctcaact tcacgtcctg gtgtttgtac attatctttt attattatat
    38761 atttaaatgt tacataatat aatacaaaga atataattcc tataggtata actaaccatg
    38821 ctttatctcc ataactaaaa cttaaaacat aatcaattat acttgctcca ttaaatgaac
    38881 ccaagcgagc acccagtaaa tatagtacaa ctccacctag accagaataa aaagaatgaa
    38941 ttatatataa ctttggagat gcaaataaaa acgaaaattc tataggttca gttactgttg
    39001 ccataaaaga agtcaatgcc ccagctgtca ttgctccttt tacttcttgt ttccttttat
    39061 cttgtgctgt tttatatata gcataagaaa tagcaggtat tgagaacatc ataattatgt
    39121 aaaatcctgt ccagaagaat cctgcatttg ggtctcctgc cataaatcta ggaatttctc
    39181 ctcttataac ttctcctgtt ggtgttgtat attcacccat ttcaaataat atgtaagcat
    39241 ttaaaacttg atgtaaccca gttggtatta gtgcacggtt acagaataaa aataagaata
    39301 ttcctattgc accagctcct cccaaccaaa aaccaaattg attcattgca gcttgtggat
    39361 atatccataa taaactaaat atatatgata aaattaaagc aactactggt gctagtgtaa
    39421 ttacacattt ttctcctgca aagaagctta aaaatgctgg aactttaact ttatatgttt
    39481 tattgtaagt ccatgctata caggctccaa caattatccc accaaataca cctgcattta
    39541 atgtatcatt caataactct aaatttgact taaatatcat atatgcaact actgctgcaa
    39601 ccattacact tgtcttatct ttagactttg agtaggcact agctgctcct aataaaaata
    39661 agtagtccac atttgaaaat actatagtag atatattaga tatagctggt acattaagca
    39721 tatttggtcc aaataataat cctaatatac ctgctgctgg cattacagct attggcacta
    39781 gcatagtttt tcctaaaagt gacagttgtt gcatcatttt gtttaagaat ctcttcatat
    39841 ccaatcccct ttctatttcg gtttaatttt ttgtgaaaac gatttgttta cattttatat
    39901 tttaaatatt atatttgttt aaacaaattg tcaatatttt ttatttattt tatcttcatc
    39961 atttagtttc acatctaaac tcttttgagt atattgctat atctagccta tttttttagt
    40021 tcattttttt tatttttaaa atatgtttaa ccatttattt gaagtaagct acaaaatagt
    40081 tcagttttat cttttttata gcttatataa ttctattttc actatacatg tctaaacaca
    40141 tttgatataa tttgttcata tcaattcaat aatattacta aagaccttta aggaggggta
    40201 ggggtatatg gctaaaaata aattttcaga gatatatgaa gtattaaaag aagaaatact
    40261 tgatggtaaa tatacaagta atatgatgtt gcctactgag cttcaactta ttgaaaggtt
    40321 ttcatgtagc agaaacactg tacgtagggc aatttctcaa ttaaacgcag aaggatatgt
    40381 tcaaagtata aaaggcaagg gtgttgtagt gttagaaaat agttgttcta atgatttttt
    40441 tctaaatatg cataatttca aaggtgtaga atcgattgta gaagataaaa aggttaatac
    40501 tgctactagt gttctccact tcagcaaaat cttaattgat aataagttat ctaaaaagac
    40561 aggttttaag attggtagtg aagtatatta tcttcacaga ctaagatata ttgataatgt
    40621 acctaaaata ctagacatta actattttct atgcagtata gttaaagacc ttgatgtttc
    40681 tatagcacaa ggctctattt ataaatatat agaagagtgt ctaggtacaa aaattgtttc
    40741 atccagaaag atatttaaaa ttgaaaaagc tacagagctt gagttaaaaa cgctacctct
    40801 aaatgattat aattgtgttg gtgttattaa aaatagtgta tatactgatg atggaaagct
    40861 ttttgaatat actgaatcaa agcatacacc tgaaacattt gtattcatgg atgttactca
    40921 aagatattaa aaaaattatg tttaattaaa cataattttt ttacgtttag acactttata
    40981 ctatactaat ctcactattc tataaataat tatatgttta tacaaataat catttatttt
    41041 atattttacc tttagtttca gttttattct tgacaatttg tttaaacata ttataattaa
    41101 cttataaatt acaggaggta tcaagtatga aaaattggtg gaaaaaagca accgtctatc
    41161 aaatatatcc aaaaagtttc aaagatagca acaatgatgg tataggtgat ataaatggta
    41221 ttatagaaaa attagattat ctttcttcat taggtgttga tttattatgg ttaactccta
    41281 tgtacgtttc tcctcaaaga gataacggat atgatataga agattattat aatatagacc
    41341 caaaatatgg gacaatgagt gactttgaag agctcttaaa ggaagctcat aaaagagact
    41401 taaaaataat gatggatatg gttctaaacc atacatctac tgaacacaaa tggtttaaag
    41461 aatctaaaaa aagtaaagac aacccatatc gtgattatta tttctggaag gatgctaaac
    41521 ctgatggttc tgtacctaat aactggatat ccaggtttag tggaactgct tggaaatatg
    41581 ataaaactac taaccaatat tatttacatt tatttgagga aactcaagct gatttaaatt
    41641 gggaaaatga aaaagttcgt gaagaatgtt acaaaatact agaattttgg gccgataaag
    41701 gtattgatgg ttttcgtttg gatgtggtca acctactatc caaaacacct ggattacctg
    41761 atgaccctat aacagggcca aagggagatg gaagaactca ctacgctgat ggtcctcgta
    41821 tacacgaata tttacataat atgaatcaaa aagttttcaa acctaaaaat atagttactg
    41881 taggtgaaat gtcatccact acacctgagg aatgtatcaa ctatactaga gaaaatcgag
    41941 aagaactatc tatggttttt agctttcatc atatgaaaac agattataaa ggaggcgtaa
    42001 aatggactaa tgaaatgttc tctttggata aattaaaaaa agcacaatct gactttcaat
    42061 ataaaatgta tgaaggaaaa ggatggaatg ctctatttta ctctaatcat gaccaaccta
    42121 gagctttgtc tcgattcgga gatgatagat tttttcgtca agaaagtgct aaaatgttag
    42181 ccataacact atttggttta caaggtacta cctatatata tcaaggtgaa gaaataggta
    42241 tgacaaatgc ctactttact aaaatagatg aatatgatga tgccgagagt aaaaatgcat
    42301 actatcatat gattaaatct ggcattgatg aaaaagaagc attgattata cttcaacaga
    42361 aatctagaga caatgctcgt actcctatgc aatgggataa taccatttat aagggatttt
    42421 ctaatcataa accgtggcta aaagttaata atgacaacat tagtgtagaa aatgagctta
    42481 atgattctca ttcagtattt tatacttatc aaagattaat aaaatatcgt aaacaatatg
    42541 atatttttac agaaggtact tatagattgt tagatgaaaa tcataaaaat ctatttgttt
    42601 atgaaagaga gtataaaaac caaaatattc ttgttataag taacttcact catgataatg
    42661 tagtgtataa attacctgaa acctatttga ataaattaaa ctacacaata cttgaaacca
    42721 attacactag agatacaatt gaaaatgaaa tggaaataaa accttacgaa agtataatga
    42781 tatattgtaa ataaagttat ttatgtaaaa atcggtataa catcagagtg ttataccgat
    42841 ttactaaatt caaaataata tttatagaat ctctataaat tacaataatt atttgacttc
    42901 tacattatta tatttctttg gatatacttt agtcatatag aaatatatag gaactacaat
    42961 tatcatccca cctaatcccc aaactatctt cataggctca tctatacctg cttttatcag
    43021 taataatata cttataataa ctgcaaatat tggtataatt gctcctccag gtattctaaa
    43081 atttacatct ttaccagcat acttctttct caatattagt acagctaagc aggtaggaat
    43141 atattgaata aatctagata caacacttat ttgagataat gttgtaaatg agcccgacca
    43201 tgctattagt agagttccta tgacagatac tataattgct atatatggag ctccattttt
    43261 attagttttt cttatagact taggcattaa tccttgttca accaaagcag cagcagacct
    43321 tggtgtaaat atggatgatg caatatttat acctccaaca gatactatgg ttcctattgt
    43381 aattatatac ccacctatag ggcctaacat cttaatggca gcatctgcaa caggagtagt
    43441 tgaacctact aagccattac ctaatactcc tatacttatt cctagtataa gcatatatat
    43501 tactgatact acaaacatta ccattaccag tgctttaggt acatttttct tagggttctc
    43561 catatctttt gctgcaacag ctaaagattc aaaccctgta aaagcataaa ataaagttat
    43621 agatactgct acaaaagctg atgatgaact ttgacctttt tgtattatta caaatggtgt
    43681 aaaattagaa ccctttataa agaataatcc tacagctata aataaaacta ttggtaataa
    43741 ttttcctata gtcattatat tatttaattg cttagattgt ttcatcccca taaagtttat
    43801 aatcccaagc cctacaacta atataccaat tattatattt ttagtaagtg gttctgctgc
    43861 ttgtggccat acagatgata gtgttacagc aaagaaattt gccatagtcg cccaagcaat
    43921 catactaact atccacttca taaatccaac ttcaaatcca ataaaatttc caaatgcctc
    43981 ttttgcataa agatatggac ccccatcagt atcaaaccaa ctacctgctt cagcaaaaca
    44041 taaagcaata gaaacagcta aaagcataca aaaaactaat actaatatac ttgctggacc
    44101 tatatctgta taagcatcac caggtaatcc aaatatacct gaccctacta ttgcattgat
    44161 tcctaatagt acaatactcc aaaaacctaa cttaatgcct ttttccattg ttattccccc
    44221 caaagccaac ttaatatagt tataattatt tttcttttga tgcttcttta actattgcca
    44281 taaatatttt tttcatatta tcataatctt tagtcatcat ttctggatgc cattgtattc
    44341 ctattacaaa ctcacttcct tccttttcta tagcttcaat taaaccgtct ttagatgtag
    44401 ctgaaacaat atatcctggt gctactttgt ttacaactaa atgatgaaaa ctattaacta
    44461 aaatttcttt ttctcctaat atttcatata attttgtatc ctcttttatc agcgctgtat
    44521 gtgtaggtat gttagataaa tgttgttgat tgtgtttaat ataagcgcct tcaattagag
    44581 ataaatcttg atacaaacta cctccctcag ctacatttat aatttgttct cctctacata
    44641 tacctaaaac tggttttttc atatctaaag catattttaa taatttcata tcaaaaacat
    44701 ctctttttgg tgatattgca cctaacttat tatgaggttc ttctttccat atgagtggat
    44761 ttatatcttg tcccccagat agtataagcg catctatatt agatacttgt tcctttataa
    44821 tctcatcatc atatacaatt ggtacaatat atggtatagc ttcgcacata actactgatt
    44881 gtatatagtc attatttaca taagctcttt catagccagg aaacattcct ccttcatcaa
    44941 ttaataaatt acccgatatt cctattattg gatatttctt tttaaccaat ttcttcaccc
    45001 cttcacttat tcgtaaacaa taaaaaatgc tacttctctt aaagagcaag tagcatacca
    45061 atattttatt tatttgaata ttataattta ttttagaaat acttttttta aatactagta
    45121 atataaataa ctttctttac ttatatttta ttttaatcct gtttagtttg acttttttaa
    45181 attgttttaa gtacaattaa taggtaaaat aggtatttat attttccttt ttattaacct
    45241 attacatgct gtttaatata tttttacccc ttctatttaa ttcacttccc cttctaccag
    45301 attcacatat tatcaagtcc agctctttta gtatattaag tattcctctt acctcacctt
    45361 ctgttatact atagcctaat tcatttattg aattaacaat accatttcta cctatatttt
    45421 tattaagatt attggcattt tttagacacc ccatcactaa ctttactttt tctaaagatg
    45481 ttctatcctt taatatactt atttctttac taaaatcttc agttatattt aataagttat
    45541 gaggtaaatc acttattaaa ataacatctt cagagctcat aatgttaata tacatagcag
    45601 tattttttaa ttctcttata ttacctggcc attcatatgc attaataatg tcttgaacat
    45661 cttgactaat tgctctcttc cttttcataa aatgcttcaa cataacttct atatcttctt
    45721 ttcgctctct taaaggcggt atatttacag gcaatacatt tatcctatag tataaatctg
    45781 ctctaaatct gtcttctttt atcatttcta ataaattttt atttgtagct gcaattactc
    45841 taacatctat atctattaca ttctctcctc caactctcat tacctgattc tcttgtaata
    45901 ctctaagtaa tttgctttgt aagtatatag gcatgtctcc tatttcgtcc aaaaatatag
    45961 ttccattgtt agcttgttca aataatcctt ttttacctcc ttttaaagct cctgtaaaag
    46021 caccttcttc atatccaaaa agttcgcttt caagtagatt ttctggcata gctgcacagt
    46081 ttattgctat aaatggttga ctatgtcttg gtgatgcatt atgaatagac tgagccataa
    46141 gttcttttcc tgttccactt tctccagtta taagaaccgt ataatcagat ttagctattt
    46201 tcatacctaa ttccttagtt ctttccatct ttagcgaaac tgtttttata tcgtcgaaag
    46261 tatatctagc aacttgtccc ttttctctaa gcttctttga taggttttgc tccaattttc
    46321 ttatgtaagt tatttcttga atacaaaaat aatatccagt aacccttcca aaactctcta
    46381 tattatactt atttacgttt aagtacttat tattaaattt tataacttca tcatgtgcct
    46441 tactcaacct aaaaatcttt ttaatattct cattctctaa ttcatctata tttttcccta
    46501 taatattacc ttgaatatct agtatattac ttagactatt attacaaata attacgtttc
    46561 ccttatctga tatcattgca atacctaagt ttgataaatt tattatggaa tttaattcat
    46621 ctgcttttat agtaagttct ttgtatttag tatttattcc tgagtataaa ctaattattt
    46681 catctgtata cttaattagc ctctcagtaa tttccttatc atccagtttg agttttgcta
    46741 ttatttgaat aaatgttgaa ctatcaatat atctatgccc taaatcaata acttcttcta
    46801 tgtattttgg aactaaactt gcctctcctg gtgttatagc tatcttaata tttctatatt
    46861 cttcattggg attatatggt ataaaattca agtgatttat tcctatatta tataaattag
    46921 atattgtttc caatacagta tgtttattat cgtttactac taatacatct attccttctg
    46981 gcaatgagaa taacttatat atatcctttt gttttatact cctatttata gttattatct
    47041 ttgagacatc ttctacatat ttttttattt ttaatactct atcatctatc attaccaata
    47101 ttatgtcatc ttttatcagc tccttttctg ttattttatt taaataataa ttttttatca
    47161 ttactttatc tttaaatatc atttctagat ttttatttaa aaaacattct aatttagaca
    47221 atccatcagt tactactgct attgttttca ttttattact ccttttaaaa tatactctac
    47281 tcttttataa tatacatttg atttccactt taatagcata aaaaattgta catttagtat
    47341 actaacatat tcatttatta gttttatttc cctaaacatc atagttgcaa tatatatcat
    47401 atttcttaag cttatacagc aaaaaatgtg ccaaattaat aattttctta cttttatttt
    47461 aaagttcttc aatcgtattt acagacttag catttataaa ttataagtct ttatattatt
    47521 aatctatttt tataggttaa ttatacaaat catataccac ttttacccat taaattcatt
    47581 ataaaaaaga tggaataacg aattgttact ccaccttttt tgtaataaaa aattgtatat
    47641 ataaacacca tttgacactc ttctaagtac aaacagtatc tacatgctat aacctttcac
    47701 cattagattc tattacttgt ttataccagt taaaactctt tttcttaatc ctagacaagt
    47761 ctccacttcc atcatcatac ttgtccacat atataaaccc atatctcttg gccatctcac
    47821 cagtagatgc gcttactaaa tctatacatc cccatggagt atatccaaac aaatcaacac
    47881 catcttctac agcttccttc atttgctcta tatgtgcttt caaatactct atacgatagt
    47941 catcatttat cactccatca ggttcttttt tatcataagc tcctaaacca ttttcaacca
    48001 ccatcatagg tattctatat ctatcataaa tttcatttag tgcatatcta agccctttag
    48061 ggtctatttg ccatccccat tcactagact caagatatgg gttttttact ccacctaata
    48121 tattgccact tgtattactt tctttagact cactacttac acatgtagac atatagtaac
    48181 taaatgtata aaaatctaca caaccttctt ttaaaatttc taaatctcct ttttccattt
    48241 ttatctcaat attattttct ctaaaatatc tattcatata agttggatat tcaccacgca
    48301 cttgtacatc tccacagaag taattcatta ctctattatc ttcttgagtc ttaactacat
    48361 catttggatt acaagtataa gggtacattg ttgcaaatat ctgcatacat ccaacttgat
    48421 aatttgaatc tatttcatga gctaacttca cagccttagc acttgctaca aattgatgat
    48481 gtaacccttg aaaacgaagt tgaggtatat ctacttggct tataaaatct gtagttcctt
    48541 catttaatat cccttgtgac aagtaccctc ccataggcat tgtagctgaa tttatttcat
    48601 taaatgttaa ccaatacttt actttccctt tgtatctttt aaatattgcc tcacagtaat
    48661 tcatatacaa gtctattact tctctcgaag cccatccatt gtatttctct gttattgcaa
    48721 atgggacttc atagtgagat attgttatta atggttctat attatatttt aagcattcat
    48781 ctattacatt ttcataaaat ttaagccctg cttcatttgg ttctttctcc attccagttg
    48841 gaaatattct agtccatgct attgaaaatc taaatacctt aaatcccatt tctgcaaata
    48901 aagctatatc ttctttatat ctatgataaa aatctattgc ttcatgactt gggtagaacg
    48961 ttcctttttc tatagttctt gttattcttt tacttactgt ttgagaacct ccagtacaca
    49021 tatctgaaca agatgtacct ttcccatcta aattccatgc tccttcacat tgatttgctg
    49081 ctgttgctcc accccataaa aaatcttttt taaatgacat atctatatcc tcctattata
    49141 attctttacc atttgattct attacttttt tataccagtc aaaactcttc tttttactac
    49201 gttttaaagt tccgtttcca tcattatctt tatccacata tataaatcca tagcgcttct
    49261 tcatttctcc agtactggca cttactaagt ctatacatcc ccatggtgtg tatccccaca
    49321 aatcaacacc atctaactca acagcatcta tcatagcttt tatatgagca cgtaagtaat
    49381 caattctata gtcatcattt atattcccag tttcatctgg attatctact gcgcctaatc
    49441 cattttcaac tataaataat ggtttttgat aacggtcata caaagaatta atagttattc
    49501 taagccctag tgggtctatt tgccatcccc actcacttgc atctaaatat ggatttttaa
    49561 gactttcaaa aacatttcct tctgttaaaa tatttacttc agggtctgca cttgtaagcc
    49621 ttgatgaata atatgagaat gatataaaat ctactgtgtt ctctttaagt atctttttat
    49681 catcttcttc catcacgata tttattcctt ttcgttccaa ttcttttaat gcataagctg
    49741 gatattctcc acgagattgt acatctataa aaaagtagtt ttccctatct ttttctatag
    49801 atttccatac atctagtgga ttacaagtat tggcataagt attccccgct gctatcatac
    49861 accctatttt aaattcttga tttatgttat gagctagttt agtaactaat gcacttgcaa
    49921 ctaattgatg atgtgcagct tgatatttta tttgttcttc attttctccc tcttcaatat
    49981 agagacctgc acctataaat ggtaaatgaa gtatcatatt tatttcatta aatgttagcc
    50041 aatactttat cttatcttta aatcttgtaa aaataacttc acaatatttt acatataaat
    50101 ctactagctt acgattcctc catgctccat atttttttac taaatacatt ggaacatcaa
    50161 agtggcagat tgttactact ggttctattc catactttaa acactcatca aacaaatctt
    50221 catagaattt tagaccctct tcatttggaa cttcctcaat tcctgttgga aatatcctag
    50281 tccatgctat tgacatacga tagcatttaa accccatctc agcaaataat tttatatcct
    50341 ctttgtaatt gtgatacata tctattgctt catgagctgg ataataatgc tcctcatcac
    50401 attcaaacat tttcattttc ccagtcaaaa ctggaaatct atcttttccc tgtggtataa
    50461 catcaacatt agctagacct ctaccacctt ctttataccc accttcacat tgatttgctg
    50521 ctgttgctcc accccacaaa aaatcttttc taagtgccat aactctacct ccaataattt
    50581 atttatagta aatttatttt aaaataaaaa tacatatatc taattctcaa ttaataaagc
    50641 cgtcctttac gctaaaacag ttatcaattc ctgactatta cttatatgct tttgtgtatt
    50701 ttctacgata tccacataat cagaataatt tgtaacgact actggagttt ctaagcaata
    50761 tccttctttt gatatgcttt ctatatcaaa agttagtaat gtctgacctt ttttaatctt
    50821 atctccttga ttgacaaaag atttgaaata cttaccttct aactgaatag tatttaaccc
    50881 tatatgtatc aataattcac aaccattatc agatataatt cctattgcat gttttgttgg
    50941 aaataaagtc attacagtcc catcaaatgg tgctactact tttccttctt ttggtgttat
    51001 tgctacacct tttcccaata cacctgttga aaatgctgca tcttgcatat gttccaaagc
    51061 ctttacttca ccttttattg gactatatat tacttctctt ttgttttttt ctacagcgcc
    51121 tttactatta ttatctttgg aaatttctac tatagtatca tctttccaaa gaaagaatgt
    51181 taatacaaat cccaccacaa tggctactat tatagatatt gtcgttgcat aaacacctgt
    51241 catatctcca gttgttggat ttatataatt aggtatacca aatattccaa gtccacccat
    51301 tacataactt tttacattca ttactcccat tacagctcct gctacagcac cacctacaca
    51361 actgtatatg aatggttttt tccttggtaa agttattcca tatattgcag gttctgttac
    51421 accacatatt cctgaaataa ttgcaggtat acacaactcc ttcattttct tatcttttaa
    51481 tttaaagtac attgctgcca ctactgctgt ttgtgcaaaa gatggtacaa atgctcccac
    51541 taatattgtg tcaaatccca tagtcataag gtttgacata gctattggta ctagtcccca
    51601 atgtagccca aatattacaa gcacttgcca caataaacca gttaatgcgc cacatagaat
    51661 tggtgaaaag ttaaataaag ctaaaaatcc atttccaact atattagaag cataggtagc
    51721 tataggccct ataaccataa atcctaatgg taaagaaact agtaaaactg ccattggtac
    51781 aaagaaattt tttactacat ctggtattat tttagcaaac attttttcta acttagatgc
    51841 taagaatact attaaaatta ctggaactac tgttgaagta tagttcattg ctattactgg
    51901 taatcctaag aaagttatat atactggtga ttcaaatatt gttcctgtaa atagattgta
    51961 taaaggttga cctgttgatg ccagagtgtc caattgaata cctgaatagc acaaaactgc
    52021 acctataagc ataccgataa atggttttag cttaaatttc ttagcagctg tatatccaag
    52081 aattataggc atatactgga acattgcatc accaattgca tttaacataa tatacgcacc
    52141 atcagtttct ccatacaaac ccagtgctgt aaatactgca ttgaaacctt ttatcatacc
    52201 agcagcacac agtactccta gtattggttg gaaaactcct gatattatgt ctataaactt
    52261 attaagtaat ccttttggtg aatctgaacc tgaattatct attgtatctc ctcctaatcc
    52321 agccaaatca cacacaactt cataaacatc tggaacatgg ttaccaataa ctacttgata
    52381 ttgacctgta cttttcatta cagtaactat tccatccatt ttttttagta catcatcatt
    52441 tgctattgac tcatctttta acttaaaacg tagtctcgtt atacagtgag ataaactgct
    52501 aatattatcc tttcctccta cattctcaat gatttctttt gatagttgtt catactttct
    52561 cattttttat cctccatttt ctttttgaaa tatgaaggtt tggccaactt aatgtcacca
    52621 cccaatatat ataaacctaa ttattaggat ttatatctga tttaatactt ttgcttacca
    52681 ccctttcgac atgaatagtt aagtataatt tttcctcatc tgttaaattg taattgtatt
    52741 ttttatgtat aaattcatct aacttttcta tgcatttata agcattttta tatttgactt
    52801 ttattacctt taataaatca tcttcattat tttcatgtaa attattactg acaactcttt
    52861 ctgcaaagta ctttaagtga gtgataaatc tatgataata aactgaatct tcatcaaata
    52921 ctattctata atggtactta accacatttg cgatttcttg tattatttgc gtaatatcat
    52981 atataatttt tatactttga tccatttgtg cattaactat atgtagtgca ataaatcctg
    53041 cttcatcttc tggcaatcta atttcaaatt ttttttctat ataatctaaa gcctttaaac
    53101 ctatgttaaa ttcattttta taaaatcttt ttatatccca aagtaataca ttttttacat
    53161 ttactccttc acgagctctt tctattgcag tatacatatg gtctgttaaa gaaatataaa
    53221 tactatcatt taatcttttc cctagttttt ctttagcata taaaattatt tcatctgata
    53281 attccatata tttcatagga atatctgcta ttaattcttt aaatttatca catatatttg
    53341 gatctgaaag tttgaatatc ttatcaatct tatcctttga tatatggtct cctgtacgtt
    53401 ttttataagc taaacctctt cccataacta ctatttcctt atgattttca tcagttgtaa
    53461 taattacatt attattgagt attttttcaa taatcatttt gatgcctcct ttattagcat
    53521 taagttaaaa taaatctttt ttaattattt agaatattta aaatgtaatt tattacataa
    53581 aaaaaaacct atttattaca cattgaatag actcccatga gccctgagta ataaataggt
    53641 ttagcttgta tttaaacaat cactatccgt ttgtatttta ttttaattat tatttattat
    53701 atgttagcaa atttattttt tagtgtcaac tttttcttaa tattttaatt tttaataaac
    53761 tatataaatt gaatccacaa aaatattatt tttatcaata atcaatataa aaaaaggtat
    53821 tacttttttt aagtaatacc aatttatttt taatttaagt gatttttttt aatctcatca
    53881 agtagtccat cttctaaata aatttctaat gcacatccta ccattgcctt tactgctttg
    53941 tgaagtttgt cataagcttc ctcagaattt gcatagctta gaaactcttt ttcatgaaca
    54001 tataccattg gatttatatc taaagctatt tctgtataca tagtaggaca aacttgactt
    54061 acattcccta tgtctgtaga acctactgga ccatctttac cttctaatat atctgttata
    54121 cctgcctcaa tcaaattatt ttttgttatc ttttgaagaa ctttattatt tattatgttg
    54181 tcaaaagaat tttcaaaata tctataagaa agcttcgctc ctgtcattaa ctctgcacct
    54241 tttgcacagt ttataacctt tttagttact tcatctaaat aacttctttc agcagctctt
    54301 acaaaaaact tacaggctgc tttttctgga actatatttg gagcttctcc tccatttgaa
    54361 actattccat gtattctaac atctgatttt acatgttgtc ttagtgcatt tatcccagca
    54421 aaagtaagtt gtactgcatc tagtgcgttg actcccatat gaggatgact agccgcatgt
    54481 gctgataccc cttcaaaact aaactctaat gaatctattg ccaaagcctt gcaatttata
    54541 ttattaaaag cttctatatg catttgataa cttacatcaa catcatcaaa agctcctgcc
    54601 ttaactaaat cacattttcc tcctgttgtt tcttcagctg gtgttcctat tagtacaatt
    54661 tttcctttaa aattttcttt taacttactt aatactatac aagctcctag tgtagatgct
    54721 gcaatccagt tgtgcccaca agcatgagct ggttctttat tttctccata tccaggaagt
    54781 gcatcatact cagctaaaaa tgctatagta ggtccatcat tatttcctaa ttcagctcta
    54841 aaagcagtct ccatattgca atacggataa attgtatcaa atccatattc ctccattttc
    54901 tctactaagt attttgatga tatatattct tcttccccta attctggatt gttaaatata
    54961 aaattacaaa cttcttcaat ttcttttcca agttcattct ctatctcata agctcttttc
    55021 tttatactct ctatcataac ttacctccta ctagaatcct agatataatc ctattgtcaa
    55081 aaataccatt acaagaactg tcaatgctac cattaaagga attatgaatc taacccactt
    55141 tgaccaagga atattggcta ctgctagtgc tcccattaaa acacctgatg taggtgttat
    55201 taagttagtt acacctatac caaattgata agctgtaact atcatttgtc tagatacatc
    55261 tactaagtcc ccaagtggtg caagtacagg tattgtaagt gatgcaagtc ctgatgctga
    55321 tggtattaaa aatgctatta atccttctat taagaaagtt aaatttataa atatagtttt
    55381 tggtaatccg tttaataaat ttgctgcaga atttaaaatt gtatctatta taaatccatt
    55441 ttctgctatt attacgattc cacgtgcaaa tgctatacac aatcctgctg tcattaaatc
    55501 tccagctcca cttataaatg aattaactat atcatcttgt ttaagacctg atgctatacc
    55561 tgatattata cctatagctg taaatatcat tgcaatttca gttatatacc atcccaactt
    55621 taaaactcca tatatcataa tcgccatacc tattgtgaaa attgctatta ccatggcttc
    55681 tctttttgta aattctttta tgtttgaatc actattaacc attgactctt gatttagaga
    55741 tatatcgtgt actaaagatt ttttagggtc ttttttaact ttattggcat acatcataac
    55801 aaaccctata gatatagcca tatatattat aaacataaca actctaaact ctattccaga
    55861 acctggagtt atttgtgata atgcttgtgc tattcccaca gagaaaggat tagtagtagc
    55921 tgctccaaat ccagctgttg ctcctataaa tactgtggcc actgctgtca atgaatcata
    55981 tcccatactg gtcataagtg gaataaatat catataaaaa ggtagtgttt cttcactcat
    56041 tccaaatgtt gttccaccta gtccaaatag aatcattgaa ataggtatta ttaaaaattg
    56101 aaacctcttc attttattta cggtatgagc tataacagct tctatagccc ctgttttatt
    56161 aactatccca aaagctcctc caactattaa aacaaatcca actacttcag ctgcatctat
    56221 aaatccgttt ataggtgctg taaatacatc agtaattcct tgtggatttg atggtgcttt
    56281 ttcataagtt ccagctacaa caactgacct tccatctata tctactcttt caaaatttcc
    56341 acttggtact atccaagtaa gaattgccat taacactatt agcaaaaata ttattgtaag
    56401 tgtctgtggc atagtaaact ttttcttttt taaactgctc atttttaatc tccccacttt
    56461 ttaaattaaa atattttatt attgcatccc tttatcctat tttgtataga tgtaaaaaag
    56521 ctcaacatgg atacatgttg agctaaatgt gctaaataca acatacctgc atcttccctt
    56581 tatcttgttt aagatagctc tccatcaaaa ttcgggataa ttttgatgac agtactgtac
    56641 ctattaaata cagtcccagc actagcttcg gacactagta cttcggcact tactcctttt
    56701 atattgcctc attggtttcc gacctcttca atattaatct tagtaaatgc aacctctacc
    56761 tcaatctttt tattagatga ggtaattctt attaattttt attaattcaa agtaattgta
    56821 gcataatatg tcgagattgt aaatattttc agtataataa ttcttacttt atgatatgat
    56881 tcaatttttc acaaataatt ttttctccat ataatttaaa taatgtagca tattttgaat
    56941 acacacaaat tttccatgaa tttcaaatta ttttaatacc cttataataa aaaattaaag
    57001 ccatgtaaac ttatcaattt acacggcttt atatacatta atctacacca agtaccgctt
    57061 tgagttttga tgctattgta ataactcgtg gtccaaatat aacttgaact gagtttttct
    57121 ttttaacaac tccacttgca cccaattcat ttacccagaa accatcatca tagaatccat
    57181 caattgaatt taactctacc ctaagtctag ttgcacagtt tgtaacattt tttatattac
    57241 tacttcctcc taaaccttct attatagagt gtacaagctc tgtatcttta tcatctactt
    57301 catctcctac agcattgtta tatactgttt taccagcatc tacagattgt ttttctctat
    57361 attctttctt gctataaagc ttaacttctt ctgaacttct tcctggtgtc tttatatcaa
    57421 acttcttaat tgcccatgta aatattatat aagtaactat aaaaaatatt ggtatcaaaa
    57481 ataataatgg taatgcatcc actttttggg gttgcaatag attaggaaac atatttctaa
    57541 atgattcccc cattatagaa acattaaaca tttctgtaaa tacatacgtc aatcctgcta
    57601 gtggtacata tgctaaaaag tacaataatg gtgcaacaaa taagaatgta tactctattg
    57661 gttccgttac tcctaccaac atggctgtta ctattgctgg tattaatata cttattgctt
    57721 ttttcttatt gacattatca gccaacttat acatagctaa agctgctgct ggtaatattg
    57781 caaaatgtat taataaacgt cctgttgtga agttacgagt tatataagat agtgtatctg
    57841 ggtctcccat ttgagctaat cttatattgt taacaccctc gtaaattgct ccacctactt
    57901 ccattgttcc accaactctt gtatattcta taggaaatgc aattaagtga tgtataccaa
    57961 atggtaataa tgctttatcc attgctccaa aagtaaatgt tcctaataat ccactagaac
    58021 ctatccaatt accaagttta gataatgcca accctactgg tggccatata aagtacattg
    58081 ctgtacccaa tactattgat gctataaaca tcataatcat tgaaaatctt ggtcctgaga
    58141 aaaaagataa tgcactagga agttctatgt caacaaattt gttatgtact gcactagcta
    58201 caaatccagc tattatagct ccaaatatac tacaatcata tgaaaatatt cccaagaaag
    58261 aaccatataa ggaacttcga ctatatgctt ctgcctcgcc taatccagat gccataaacg
    58321 cttctgctgt aactgtatct gaatttatac catttgctgc tagcaatgta cttataactg
    58381 tgtgcattcc tataaataaa acaattcctc ctaatgcacc ccatcctttt tcctttttag
    58441 ataatccaaa agctatacct gtaacaaaga atattggtaa atatctcatt accataaacc
    58501 ctaggtcgtt tattacttta aagaatacat ttataaatga cccttctttt attattccgc
    58561 taagccctaa tgcataaacg ttttccatat ttgcaaaagc tgaccctaac cctataaata
    58621 ctcctgctac aacaagtaat ataacaggaa tcatcattgc tccagaaagt acttgtaatt
    58681 tatccttcat agtattattt cctccatttt tattgaaatg ccaggctata taatgcatat
    58741 atatatactt ctattgcttt gtataatgta tctatactta tattttcatt tgcctgatgt
    58801 gaatttggat tatctccctc tattgtgggt ccaaatgcaa ctccattttc taatactctt
    58861 gcataagatg cagctccttt tgtaaaaagt tctgcctctt tttcaaaacc ttgtttatat
    58921 gcacttgata atttatttat taattcacta tctggttcta catacagtaa ttttaaattc
    58981 ttatatatat caattattaa attttctttt tttccaattt cattaattct attagttata
    59041 aactccaaac ttataccctt aggatatcta aaatctattt tcatattcaa ttcattttta
    59101 tctaagaaga tactcattat acatattgta gaaacgccca tatctacatc ttctttatat
    59161 atccctattt tttttccatg attatcatct gtaaagtatg aatttaaaat atcctttatt
    59221 ataactcctt tatcgtttag ccctttaatt ctctctaaat cttttgctaa actaaatgca
    59281 cagttatatg acctgtgtgg atttctcgac aaagctcttt ctcctgtata tcttactaat
    59341 acttttcctt cttctacttc ttctatttct gtataataag ctaatgattc tactaaatct
    59401 ttcttattat tagttttaaa gacagcctca attttgtcac atacaaatcc atcttctttg
    59461 tttgacttta tatcaactaa attgtgattt ctaaatttat ctttatctat tttctttctt
    59521 agattgaagt ataaaatacc tttttctcca tttactattg ggaagtttcc atctggagaa
    59581 aatgcaaatt ttggctgttc atttacacta aaataatgtt ccatacattc ccaagtcgtc
    59641 tcctcagctc ctcctactat caatctaatc tttctctttg gcttttcatt aagttctctt
    59701 aagaaaagta atgcatataa aatccctatc aaaggtcctt tgttatcatt aactcctcta
    59761 ccataaagat agttatcttt ttgacatact ttaaaaggtt tactcttcca aagttcttta
    59821 ttatatattg gcaccacatc tatatgatta agcaccccta ctacttcttc tccttctcca
    59881 tattctatat gaattgcata tccatcaaaa tcttttacaa caaaatcttt gtcttttgct
    59941 attttaataa gcttatcgaa ggcatttctt atttctatcc caaatggttg atttatatct
    60001 gttgtacttt catctctaat tgaaggtatt tcaactaaac tcctaatatc ctttatgata
    60061 ttttcttgat tggattttaa ataatcaata attagttctt tatttactat catcttaatt
    60121 atttatcacc cctttatatt agttttacaa agcatttcag ttcatattta cattataaat
    60181 tttgagtatt attttttcaa tatttcaaat acttctctaa attaccataa tgatacggta
    60241 aataatcaac agattatgac aaaattatac tatacctact attaatattt tatcttatct
    60301 attacatctt ttttaatgta attttattcc attaaatgat attaaaagta aaacattaga
    60361 gatattttaa atatataaaa aatggatgta ggtaaattta tttttatgta aatctaccta
    60421 catcccatga ttttattaaa caattatatt ataatattca tatctatgtg ttctatacct
    60481 ttaatagtta tagctttatc taatacttta ctaatcaact aattaaattc aaaagatttt
    60541 cttttaaata acattctaaa ttaagttaca taaatcttga ataaattgcc tctgttatta
    60601 aaacatctga ccccataata taaaataaat acatacactt tacaacattt taattttgta
    60661 aatactaatt tcatgtatat aagataaatt tcctattaat attaataaaa acattataag
    60721 gccat
//
LOCUS       DAMJVM010000012        60214 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.012, whole genome shotgun sequence.
ACCESSION   DAMJVM010000012 DAMJVM010000000
VERSION     DAMJVM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 60214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60214
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.012"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(55..819)
                     /locus_tag="PC523_000828"
     CDS             complement(55..819)
                     /locus_tag="PC523_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2474383.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSRPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVNVL"
     gene            1092..1766
                     /gene="fsa"
                     /locus_tag="PC523_000829"
     CDS             1092..1766
                     /gene="fsa"
                     /locus_tag="PC523_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDF2474384.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSATVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(1888..3282)
                     /locus_tag="PC523_000830"
     CDS             complement(1888..3282)
                     /locus_tag="PC523_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDF2474385.1"
                     /translation="MGIILKPKQFYITVIATIILYFSLNYNFNIKFSICLSFLTILIW
                     AIDSVEKTVVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFVSHENLEVNKIQFSYSNFKKELNLLNKEQKYVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     RENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWRFIGLEI"
     gene            complement(3361..4707)
                     /locus_tag="PC523_000831"
     CDS             complement(3361..4707)
                     /locus_tag="PC523_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2474386.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLRLMNTPESVFSQSKLYLTIIFGGILFSAGYNSVSAILRGLGDS
                     ITPLYFLIIATILNIVLDLTFIVVLKMGVEGVALATIIAQAVSFIISIIYLNKKHEVL
                     KFKIKGIIYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVIVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSRFFGTNGIWMGIPFGWTLGLIVTVIYYNKGRWKTKAIVNHRINK"
     regulatory      4957..5059
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5153..6529
                     /locus_tag="PC523_000832"
     CDS             5153..6529
                     /locus_tag="PC523_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDF2474387.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEVIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCIGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFM
                     NPEHAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPKNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSIVMIVLAIL
                     FIIKLWLL"
     gene            complement(6631..7776)
                     /locus_tag="PC523_000833"
     CDS             complement(6631..7776)
                     /locus_tag="PC523_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2474388.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAEISVT
                     SLALIGEVNIQKQIKNIKSVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKTLTATNPKRAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MAKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRNGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELVDIIK"
     gene            8028..8735
                     /locus_tag="PC523_000834"
     CDS             8028..8735
                     /locus_tag="PC523_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2474389.1"
                     /translation="MLKVINLSKSYKNKKVVNSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFENGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            8782..10377
                     /locus_tag="PC523_000835"
     CDS             8782..10377
                     /locus_tag="PC523_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2474390.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKSDGREKQKIMGMILSAIIITG
                     LLIFYVTTLSLSLVDILKQINQMEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDI
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFMFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKIYPPIYYFVDALKTGSVVSFLAFIAISIIPFFMFVTLFAKKFKNINAK
                     MSEGYKSKTYKFKSQKTSRPSKALLKKEFKRYTSSFIYFLNSSASLLFLIVGAILILL
                     FGADKMGELLKLNIDFNFIKIQLLGVFFFIIVMNCTTYCSISLEGENLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMVITMLVSLAYVVILGGIYTLLGISFLNIYIILASML
                     TLILDFMIWRIISTKGVKIFKNL"
     gene            10480..11562
                     /locus_tag="PC523_000836"
     CDS             10480..11562
                     /locus_tag="PC523_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2474391.1"
                     /translation="MRKLSKKEKDFIQYGFLLFLICITTYLVLTTLDIKMIPKIIRLV
                     NKTYIFLGILIIIIYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLIEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKMFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYIHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSSIIPKELLGYSVLLYSGFVYYFILIVSGIFTFG
                     THYTLNSWKKETKVNSNLDKVIEKLN"
     gene            11711..11872
                     /locus_tag="PC523_000837"
     CDS             11711..11872
                     /locus_tag="PC523_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HDF2474392.1"
                     /translation="MKQDKKNEKQSEISKIKQSNDNKLEVAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            12214..13482
                     /locus_tag="PC523_000838"
     CDS             12214..13482
                     /locus_tag="PC523_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF2474393.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            13676..14464
                     /locus_tag="PC523_000839"
     CDS             13676..14464
                     /locus_tag="PC523_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2474394.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSNLNLDYIKEDEDCVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMKQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASRDYRKAMSKVLLKRAIMEVI"
     gene            14464..14913
                     /locus_tag="PC523_000840"
     CDS             14464..14913
                     /locus_tag="PC523_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HDF2474395.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKAGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRVLDREITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVIAMKDELENPTDEEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            14917..17181
                     /locus_tag="PC523_000841"
     CDS             14917..17181
                     /locus_tag="PC523_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2474396.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAVDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNNTVVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDIVIEETYYTQAQAHAMMETYRAYNYLDHTGRLVVV
                     SSTQIPFHVKRHLSRALELPSSKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKIDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKYLDNGKVRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLETSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGANKLGVDKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDKE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGHMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMAMNK"
     gene            17321..17629
                     /locus_tag="PC523_000842"
     CDS             17321..17629
                     /locus_tag="PC523_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474397.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKAKVFFKEK
                     QNQIEIDFVEEEISKLIENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGRVH
                     "
     gene            17915..18415
                     /locus_tag="PC523_000843"
     CDS             17915..18415
                     /locus_tag="PC523_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDF2474398.1"
                     /translation="MRKNNINIALTSAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLI
                     IASLWPTIINSIVVGLMLNIFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     IINIKF"
     gene            18468..19454
                     /locus_tag="PC523_000844"
     CDS             18468..19454
                     /locus_tag="PC523_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF2474399.1"
                     /translation="MSKIKRLTDVWTYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMAMLFALKSLQVYNYKSIEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANNSNDGEFKSINVTEFFRASSESLILNIKY
                     KFGNQILEKKICPIGIYLNKGAWFSPAYDCKIDKIILVHIDNVIDIKRTGESKKIHIN
                     LEEWLDEVYNNLYENRSTTKKIGETIYLNVLLTKEGVSKIKDSSYNLDMELNEDGSGT
                     ISTFIRVEDVDYYASILFLIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            19504..20226
                     /locus_tag="PC523_000845"
     CDS             19504..20226
                     /locus_tag="PC523_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HDF2474400.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEIEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIKEIIKAISYMSNERRVLSIGMDCSEKENKEALE
                     LGREFKINNDSIFKFVNLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKEYSEMADILFDRMEYIGEEFISKKGIVFVEGSSEFSIMELNDLINNIQRKIEESYE
                     VIFSLYMKENLDGNIRIGLLYN"
     gene            20255..22189
                     /locus_tag="PC523_000846"
     CDS             20255..22189
                     /locus_tag="PC523_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2474401.1"
                     /translation="MSENIRVTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFGMGKKSEKYINENCNDKDFIKRFVSIKKGDYFLYKAYQNIEEKVKCNVSIGMVEE
                     DFENGYELSHILGHTLPVKWMYNFDTVSNCKVYTSEFYKLNNNDISSYLEIINSQDKK
                     SLRKRIEESENNKWYLKNYYYKNVDKCVNDEELFIQPTNLEQLENIKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSTLEDGYFFPVRWIEYEKENIDGNAWV
                     IFENNSMVEKYVKRFTNKIPLNTILYGPPGTGKTYNTKQMVYNLVNKTSLRNRYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYDALKFEKKM
                     SFELEKDSLTLEAIKKRKKKLVLENIEYSDSFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFFLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPNYDLLSIIDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEELIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            22182..23450
                     /locus_tag="PC523_000847"
     CDS             22182..23450
                     /locus_tag="PC523_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HDF2474402.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENIILEILPKLSLSDNLVKDREILLQMLSICNKIPII
                     MNEKIRLSLKNYNLLNFFIMYFIESMQIQMKKGIYFEYINKIENLNVIRGKILLSTYA
                     KEKCISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILNYFQNVD
                     LIYIDRKKLLDYKFCKNNDRFRDCYLLARLLLLNLSMDNSQDNEEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKSEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITTYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKTVRLDDIKNTKNDLKKIIFNYKF"
     gene            23768..24808
                     /locus_tag="PC523_000848"
     CDS             23768..24808
                     /locus_tag="PC523_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2474403.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFARIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKIIE
                     YFKLNNINIKLLEENDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            25164..26486
                     /locus_tag="PC523_000849"
     CDS             25164..26486
                     /locus_tag="PC523_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDF2474404.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWSVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            26783..28144
                     /locus_tag="PC523_000850"
     CDS             26783..28144
                     /locus_tag="PC523_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HDF2474405.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGAQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSVTSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            28217..29611
                     /locus_tag="PC523_000851"
     CDS             28217..29611
                     /locus_tag="PC523_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2474406.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKRVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMIELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNIILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            29673..30143
                     /locus_tag="PC523_000852"
     CDS             29673..30143
                     /locus_tag="PC523_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HDF2474407.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLARNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            30146..32362
                     /locus_tag="PC523_000853"
     CDS             30146..32362
                     /locus_tag="PC523_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2474408.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGALRFYEHNIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAIKLIKVEYEELPAVFTIQEAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKELGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKRMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLITGAHDMGNGVVTMQTMMVAEILGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKVLILEEAEKLLETSKEDLYLDNGYVISKL
                     NENIKASLSDVAVYSQSKSLRELTVVEDYSSPTGLTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVNSYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            32442..33113
                     /locus_tag="PC523_000854"
     CDS             32442..33113
                     /locus_tag="PC523_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2474409.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            33476..34690
                     /gene="dpaL"
                     /locus_tag="PC523_000855"
     CDS             33476..34690
                     /gene="dpaL"
                     /locus_tag="PC523_000855"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDF2474410.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGKYLAQRLDTDISELGY
                     DKLTSDEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHATAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            34816..36003
                     /locus_tag="PC523_000856"
     CDS             34816..36003
                     /locus_tag="PC523_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HDF2474411.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNKDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGVNAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKSV
                     YGK"
     gene            36136..37506
                     /gene="hydA"
                     /locus_tag="PC523_000857"
     CDS             36136..37506
                     /gene="hydA"
                     /locus_tag="PC523_000857"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HDF2474412.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDENIVEIGNNLEINGDKVID
                     ATGRIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKAIGNCYCDYGFHMTITCLDKDTFKHMEKLISDGIVSFKMYMAYEGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLNVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCKESLKTVKMAREKNVDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINCLWEGLASGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISANKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKIKCKVEDVFLRGNHVVQSCNVKEQPIGQYI
                     KRKITK"
     gene            37524..38930
                     /locus_tag="PC523_000858"
     CDS             37524..38930
                     /locus_tag="PC523_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2474413.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHTHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTERD
                     MELAKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTTDEDKALKEAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            39003..39449
                     /locus_tag="PC523_000859"
     CDS             39003..39449
                     /locus_tag="PC523_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HDF2474414.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVDGQHITTLEGVLDEAKEFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            39488..40282
                     /locus_tag="PC523_000860"
     CDS             39488..40282
                     /locus_tag="PC523_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2474415.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKNLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMSLADFLESELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVLRMYNSIGGECDGK"
     gene            40272..42563
                     /locus_tag="PC523_000861"
     CDS             40272..42563
                     /locus_tag="PC523_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2474416.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPSYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSRALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGKTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVKDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKRKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            42564..43877
                     /locus_tag="PC523_000862"
     CDS             42564..43877
                     /locus_tag="PC523_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2474417.1"
                     /translation="MIAIKSKYLISSADEIYTNYAVVVENDIIKDILPNEEVEIKYKN
                     IEEVIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAVLSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDKFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAHVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWNF"
     gene            43913..44998
                     /locus_tag="PC523_000863"
     CDS             43913..44998
                     /locus_tag="PC523_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDF2474418.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASVGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            45228..46277
                     /locus_tag="PC523_000864"
     CDS             45228..46277
                     /locus_tag="PC523_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474419.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEV
                     RQEMTVMKEDMSEVKQEINIMKEDVSEVRQEINILKEDMLEVKQEINIMKEDMSEVKQ
                     EINIMKEDMSEVRQEMTVMKEDISEVKQEINVLKEDMSEVRQEMTAMKEDMSEVKQEI
                     NVLKEDMSEVRQEMAVMKEDMSEVKQEINVLKEDMSEVRQEMTVMKEDMSEVKLEVTV
                     MKEDVSEVKSEVNFMQNKINNINKDMSGIKEEVSIANEKLDGIEIKIDSLESEDKSMK
                     EMQVEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKEDIAEIRRDLNLVELA
                     TSKNWSDIVKLKSVK"
     gene            47611..48969
                     /locus_tag="PC523_000865"
     CDS             47611..48969
                     /locus_tag="PC523_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HDF2474420.1"
                     /translation="MKAKSTSKFELDGMPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALIFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVAGKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNQINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLIFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKIVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLERFPKFVETLLADSSVVSGAMVAIIIQALYMVKFPKNKNGKVEDKNK
                     SL"
     gene            49076..49870
                     /locus_tag="PC523_000866"
     CDS             49076..49870
                     /locus_tag="PC523_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2474421.1"
                     /translation="MVTIKEYSVPQSLEEAYKILTSKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDEKNILIGADTSLRSLEMDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGMLELNEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSILNSAMLKENNTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            49863..52169
                     /locus_tag="PC523_000867"
     CDS             49863..52169
                     /locus_tag="PC523_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2474422.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDRVRFVGDE
                     VALVAAEDEKIALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     NNNIVSKEFFEFGDVEAKLKESDLVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFALDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTSGKAAEGEILSCELKQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGILKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPRFDKD
                     GRLLTNSFLQYKVPTKKEVGNILVDFAENFDSGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRIRELPMTFEKVYMGMKNNNKQL"
     gene            52308..53585
                     /locus_tag="PC523_000868"
     CDS             52308..53585
                     /locus_tag="PC523_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2474423.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGTLNVPMSPVFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCIKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFANDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            53768..54490
                     /gene="yqeC"
                     /locus_tag="PC523_000869"
     CDS             53768..54490
                     /gene="yqeC"
                     /locus_tag="PC523_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDF2474424.1"
                     /translation="MKLSNIIEKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIITTIDGTIIPSICHGIIVCGSHINNENKLVSIDSSILDEIVDKFDLV
                     LIEGDGSKRKKLKGWNTKEPVVYHKTTKTIGILDITSFGMDINEENIHRVEMFKKIAN
                     LNTKSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLIKNI
                     KECESEIEIFYGSVKQKFCARY"
     gene            54608..55177
                     /gene="mocA"
                     /locus_tag="PC523_000870"
     CDS             54608..55177
                     /gene="mocA"
                     /locus_tag="PC523_000870"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDF2474425.1"
                     /translation="MINAVIMASGFARRMGVNKLLLDYKGCSIIEHVFRVVNKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANVGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDICGNPVIFPYGKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKIDYERIGGKL"
     gene            55174..56193
                     /locus_tag="PC523_000871"
     CDS             55174..56193
                     /locus_tag="PC523_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HDF2474426.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKDVI
                     KNAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
     gene            56531..58156
                     /locus_tag="PC523_000872"
     CDS             56531..58156
                     /locus_tag="PC523_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2474427.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLENKEIQEQLAKMYGKDK"
     gene            58351..58803
                     /locus_tag="PC523_000873"
     CDS             58351..58803
                     /locus_tag="PC523_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474428.1"
                     /translation="MVKKRLVAIVMSLAMISVGNFSYAHSGGHNSSGSHHNKNKSELT
                     YYYYCGGHSAHQHNKGFCPYASAKSNSGVTISSAHNEKNHKKFFSEKEYNKGYEDGCK
                     GLYNLSFTEAKSEDSYEKGYLIGYNEYKAKKVYDVIKKMLDIPMLASI"
     gene            complement(59000..60112)
                     /locus_tag="PC523_000874"
     CDS             complement(59000..60112)
                     /locus_tag="PC523_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2474429.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDKETGKRKQKNMGIFDKKREASKRL
                     NEVKESLYKDGFLVPNEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPIHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDGIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSIRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVISKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
BASE COUNT        23407 a         6460 c        10831 g        19516 t
ORIGIN      
        1 ctatcaaata aaaatttatg tttataagaa attctataaa atatataatt aaacttataa
       61 aacatttaca ttaaattctg ataaaatatc tactatatca ggagtaggcg ttttttctgt
      121 aattatagaa ctaatatctt ctagatgagc aaacttgaaa tttccatcaa atttaaattt
      181 tttatcttcc ataactataa atactttttt acttgaattt attatagcct tctttgtaag
      241 accatcttca atttcaaaag ttgtaagtga agagttgaat acatctactc cgcaacttcc
      301 cataaaagtc ttatcaaact tatatttgga tataaactct atagttggag taccgacaaa
      361 cccatctaaa ctgagattta ataaacctcc agtggaaatt atatttaaat tgtttggtct
      421 tgaagtaact acatttagta tatccaacat atttgtaaca agtgttacct ttttattact
      481 ttcagctaac agttttgcaa gcaatatgtt aattgtagag atatctaaaa atatagtttc
      541 tctatcttca attaaattaa atgctttttc tgctattatt ttctttgttg ggatattaat
      601 ttcttttctg tcttttgtat cctgattctc tagtgtttct ctagctagta cagctcctcc
      661 gtaaattctt cttattgaag aaatcttttc tatatttttt aaatcttttc ttatacaatc
      721 ttctgtaaca ttaaatttag aactgagctc tttgacagta actctacctt ctttttttag
      781 gatatttaat atggcttgaa ttctttcttc tgcaaacatt ttctacctct tttccaaata
      841 tagtattata gtaaatatta taagataaaa tacaatatat caactatttt aacatgaaat
      901 acaaagttaa tttaatataa aaagcatatt ttttctaact atctatttac gatttagtaa
      961 ataaagaaat tataaaatta agaagaaaaa taaaacaata aaaaaaaata aaaaacaata
     1021 agcaaaatgt tgaaaataat gccaaaccaa tatataataa gaataaacaa agattaaagg
     1081 gggatatttt aatgcaacta atcttagata caggtaatgt ggaggagatt aaggagttat
     1141 gtacttgctt accaatagat ggtgtaacta caaatccaag tattgtttca aaagaaaaaa
     1201 agaattttaa gcaacttata aatgaaattg gtgagattat aggtgaggat atgcctatac
     1261 atgctcaggt attaagtaca aagtatgaag aaatattaga agaagcatta tatataagta
     1321 gtttgagaaa aaatatatat gtaaaaatac ctgtaacaca agatggttta agagctataa
     1381 aagaccttca cagaaaaggt gtaaaaataa ctgctactgc aatatttact gctcatcaag
     1441 gctttttagc tgcaaaggct ggagcaaatt atattgcacc ttatgtaaat aggcttgata
     1501 atatatctgg tgatggagta gctatggttt cagaacttat aaaaataata gatacttata
     1561 aaatggatac aaaagtactt gcagccagtt ttaaaaatgc acaacaagtt atagaattaa
     1621 tgcaacatgg ggtgcatagt gctacagtac catatgatat ttgtaaatct atgatgaatc
     1681 atccattaac tgattggagt gtagataagt ttatagagga ttgggagaat acttttggaa
     1741 aaggaagtaa aacaaataat atttagtatt atggtaaaaa agcagttttg ttcaatttca
     1801 caataacttg tataaaaaga cgaaaatatg aattttcttt ttaataattc cgttattatc
     1861 ttaaatcaat tcatattttc gtctttttta aatttctaat cctataaatc tccaccaagg
     1921 gaatgttata catattatta taaagtaggt taaaggaatc aaagttaaac catacttgaa
     1981 aataaccttg ctattataca gattttcacc atgacccacc attattgtag catgatgaaa
     2041 tggtaatatg tattgtatac ttactataat gtagacaaat agacataaag taacactatt
     2101 ttcccttagt atatgcaaac ttccaagcag tggtattact acagatgatg tagtaacagc
     2161 acttccaaga catatattca agattattgt gagagttatc aaaaataata tcgaaataat
     2221 ttctgaagag tttggtataa attttatcaa ataatttccc attatatcta caactccgga
     2281 atactttaac actcctccaa ttgaaaatgc tgctataaaa aaaataagga ccttccaatt
     2341 tatttctttt aaggtgctaa atccaactat ttttcttagt aataaaagaa gtacacaaat
     2401 tgacattata attaatgtat ttatgttatg tatgaactga gttaaaaaca taaaaaacat
     2461 tattcctaag cataagaata cgtatttttg ttctttgttt agcaaattaa gctctttttt
     2521 aaaattagaa tatgaaaact gaatcttatt aacctcaaga ttttcatgag aaacaaattt
     2581 tattgaatgt aattcttttt taaagacaaa gataaataaa aaataagtta taaaacaagt
     2641 aattatagtt ggaacagaca tataaaaagc ccattgcccc caatttatat tacaatttcc
     2701 gagttctagt acaacatagt ttaggagagt gtctccattt acaaaaaaca ttgatgtaaa
     2761 ggtagaggct gtaaatatac taaataaaag tatagatttt gaatcctcag ttatctgttg
     2821 ttcttttaaa aactccttgt aaaaagcaga tacaagaata accctcggaa atggttgtgg
     2881 gataaaaaat attaaaagta tacctaaaat atatgataaa agaatcattt ttttaggggt
     2941 atttacagta tttaaaatta acttctcaga aataattttt gcgacacctg tttttgtaac
     3001 tgcattagta attatgtatg caagaattat tatgtaaaaa ttttctgtaa ataaaaactg
     3061 taaaataacc tttattggtg caatattaaa gataaatgct agaattacaa aaaacaatgc
     3121 aacaacagtt ttttcaactg agtctatagc ccatatcaag atagttaaaa aagataggca
     3181 tatagaaaat tttatgttga aattgtaatt aagagagaag tagagaatta tagtagctat
     3241 aacagttata taaaattgtt ttggcttaag aataataccc atttttatat tcacctccat
     3301 aatttattat agcataatga aggtgaatac aatgaaggtg aatacatgtt tataaaaagt
     3361 ttatttattg attctatgat ttacaatggc tttagttttc caacgccctt tattatagta
     3421 gataactgtg acaataagac ctaaagtcca accaaaagga atacccatcc aaattccatt
     3481 tgttccaaaa aatctagaaa gcatataaga agctggaagt cttccaagcc ataaagatac
     3541 tattgagctt ataagggcaa acatagagtc accagcacct ttcattgcac ttgaaagcac
     3601 aaaagaagtg ccaataaata aaaagaatgg tcctattata aataagtaag aagagccaat
     3661 tttaatgaca tctttatcag tattgaatag agcaattaga ttttctctaa acagcaaaat
     3721 taaagcaata actataactg ctaaaactaa ggtcattttt atagattctc gaatgccttt
     3781 tttgactctt tcattttctc cagcacctat gttttgagca acaaaagtag aaacagcaga
     3841 accaaggttc ataataggta agcttataaa attttcaatt ctaagaccag caccaaaagc
     3901 cgccatagca gatgtaccat aactattaac taaaaattga agagccatat ttccaattga
     3961 aaataacatt tgttgaactc cacttggaag tcctaatcta agcccatctt taaatatttt
     4021 attatcatat ataattcctt tgattttaaa tttaagcact tcatgttttt tatttaaata
     4081 gattatactg attataaatg atactgcctg agcaattata gtggcaagtg caacgccctc
     4141 aacacccatt tttaggacta caataaaagt taagtctaaa actatattta ggatggtggc
     4201 tattattaga aaataaagtg gagttattga atctccaagt cctctaagta ttgcacttac
     4261 tgaattatat ccagcagaaa ataatattcc accaaatatt atagtaagat acaattttga
     4321 ttgagaaaaa acggattctg gtgtattcat aagcctaagc atgggtccac caaatactat
     4381 tccaagaata cttattaata tagaaccaat aaataaaagt atatatgtgg tatcaatagt
     4441 agcttttact ttttttaaat ctcctgaccc ataaaattgt gatactaaaa tagaaaatcc
     4501 aaggctaaca cccataagtg ctgccaccaa taaaaacatt atagggttag ctccagagac
     4561 tgccgccatt gcctcttttc caataaatct accaactatt atactgtcag ctgtattgta
     4621 tagttgttga aataaactac caataagcat gggcatggca aaaaagaata tggattttcc
     4681 ctcatgacct gtagttaaat ctttcacctt attcctccaa ataaaaaaat tattaattta
     4741 ttatagtata atcttaacat aaactttata tccaaactat aaataaattg tatcaaaata
     4801 tcatatattt ttttaaaatg taaagaatat agttataatt tgaatttaat aaattaatat
     4861 ttaatataat cgaatattaa tacaaaatca acacaatata tacgtatata ttgacaaaat
     4921 taattatata aattaaaata atattcgtaa agaaaaaatc atgttttata ctgtataaca
     4981 tataattttg acaatatggg tcataagttt ctaccggaat accgtaaata ttctgactat
     5041 gtatactatt atttagttaa ttaaatattt aattaactga attatgaagg atacggctca
     5101 gcttatcaac tactgtgtcg tttttttgta aaatttaata ggaggtgttg ttatgcaaac
     5161 aacaaacagt agtactatgc agaaactttt tccaattttg actaataaaa atgtcgacat
     5221 gaagaaggaa gtaattgcag gagtaactac ttttttaact atggcttata taatagctgt
     5281 aaatccaaat attttatctg aaacaggaat gcctgcaggt gcacttgtta caggtacttg
     5341 tttagcagca gcatttggat gtattttaat gggtgtagtt gctaatcttc cttttgcctt
     5401 agcatcaggt atgggtctta atgcattttt tgcatataca gtagttttac aaatgggtgt
     5461 tccttgggaa gtagctctta cagcagtgtt tgtagaaggt attattttta tagtattatc
     5521 agtaagtgga gttcgtgaag ctgttgtaaa tgcgatacca aaaaatatga aattagctgt
     5581 tacaggtggt ataggaatat ttattgcact tataggtcta gttaacagtg gaattgttat
     5641 tggagaccaa gccacactta tcaaaatggg tagatttaca ccagcagtaa taataacttg
     5701 tataggatta ataattattg ctgtattaga taaaaaaaga gttaaaggtt ctatactttt
     5761 tggaatagta gtgagttcat tacttgcttg ggcttttgct tttatgaatc cagagcatgc
     5821 acaaaaatta ggaatatatt tacctggtgg attatttaaa ttcgaaagtt tagcaccaat
     5881 agcaggtaaa atagatttag gatatgtact acatccgaaa aatataggtg gttttttagt
     5941 agtagtttgt acatttttat ttgttgattt ctttgataca gtaggtacat tagttggtgt
     6001 ttgttctaaa gcaaatatgt tagatgaaaa agggaatgtt cctaatgtag gtagagcttt
     6061 acttacagat gcagttgcaa ctacagttgg tgcaggactt ggagtatcca cagttacaac
     6121 ttatgttgag agttcaacag gagttattgc aggtggaaga actggatgga cagcaataac
     6181 agttggaatt ttattcttag ctgcaatgtt tttctctcct gtatttatag caattccatc
     6241 atgtgctaca gcaccagctt taatatatgt tggatactta atgcttggta cagttaaaga
     6301 tatagagttt gataatataa ctgaaggtgt tccagctttt gttactattg cttgtatggc
     6361 attaacatat agtataggtg atggtttaac tttaggtata ttaacttatg ttttcgtaaa
     6421 tatattttat aatatatttg gagctaaaaa ggcagaagac aaaaaacatg tttctatagt
     6481 aatgattgtt ctggcaatat tatttataat aaaactttgg ctattataat agatattatt
     6541 taaaatttta atttaatata aaataaatag ctgtaatttc tcatcaattt tgatggaaaa
     6601 ttacagctat ttcgtattta tttcatttta ttatttaata atatctacta gctctccatt
     6661 ttgtaaagtt ttaggtgtaa ttactatatt gaactctttt tgcaaatctt ttataatatc
     6721 caattctttt tttgttatta ttgagattgt gttaccattt ctatttcctc tagcagttcg
     6781 accacatcta tgcagatatt cgtttttact ttttggaaaa tctaagttaa aaacatgact
     6841 tacatccact atgtctagtc cacgagcaga taaatcagtt gtaattaaaa tctttgcttt
     6901 accaagttta aatttattta ttgcattctt tctatcttct tttgccatat ttccaaaaat
     6961 tcctattgct ttgtaattat gataatttaa ttttgatact agaacttcta tatttttttc
     7021 attatttaca aatacgatag ctctctttgg atttgtggct gttaatgttt ttctaagaaa
     7081 agtgaactta tctcttattt cacctaacaa atatgagtga ttgatgtttg gatttatttg
     7141 tgatttttcc tttgttttta ttatttcaaa ctctttcata agtttatcac aaatttttat
     7201 agtgctgtca gtaagacttg cagagcatcc tataatttgt ctatctctta atgttgttct
     7261 tattatgtct tctatacaag ttatattttt tccattcaat agattgtcca cttcatctaa
     7321 gacaatagtt tttatattgt gtgattttaa ctttttttgt tttatcaagt ctaaaactct
     7381 tccacaagag ccaattacta tgtgtggttt tactgatttt atatttttta tttgtttttg
     7441 tatatttact tctccaatta aagctagaga agtaacagag atttccgcat ttttagccaa
     7501 taattcaact tgatttgtaa tctgcattac taattcatgc gtaggtgcaa gtataagagc
     7561 ttgtgtttct ctttttgatg tatcaatttt ttcaaatata ggtaaaaggt atgctagtgt
     7621 ttttcctgtt ccagtctgtg aattaatcaa taaatcctta ttttgaatta tattccctat
     7681 aacaagtgac tgaacttctg ttggagaagt tatgtcttgt tttttaagtc catctattag
     7741 agtggaactt atttttagtt gttcaaatgt attcatattt tgctccttat atttaataat
     7801 tatagtcaac tattttttat tatacattgt ttaggcaaat cgaactatat attcttttat
     7861 aattatgtgt aatttgaaag taaatataaa ttatatttat acccttataa ttatgataac
     7921 tttgtatttt ataaattttt aaaataaaca aataaaagtt gtaattgttt gatgtttgtt
     7981 atatattata tataacaaat atcaaaacta taatatggag ggaaattatg ttaaaagtaa
     8041 tcaatttatc taaatcatat aaaaataaaa aagtagtaaa tagtgtctca tttgatgtaa
     8101 atgatggaga gctttttggg tttataggac ataatggagc aggtaaaact actactatta
     8161 aagcaattgt tggaatacat gattttgaaa atggagaggt actaataaat tctaaatcta
     8221 taaaaaaaca gcctgttgaa tgtaaaaagg aaatggcata tatacctgat aatccagatt
     8281 tatacgaatc attaactggt atacaatacc ttaattttat agctgacata tttgaagttg
     8341 ccaaagataa aagagaagag ttaataaaat attattcaaa taagtttgaa ctcaataaag
     8401 cattaggtga tttaatatct tcatattcgc atggtatgaa acaaaaatta gctataattt
     8461 cggccttgat acatagccct aaaattttaa ttcttgatga accttttgta ggtcttgacc
     8521 caaaagcttc ctttatattg aaggaaatta tgagggagtt ttgtgataaa ggtggttgca
     8581 tatttttctc aacacacgtt ttagaagttg ctgaaaaaat ttgtgataag attgctataa
     8641 taaaagatgg aaatataatt gcttatggga ctacagaaga agttaaagga agtaattcac
     8701 ttgaaagtat tttcatggaa ttaatagaaa aataaaatgt tttttataca atatagtatc
     8761 taaagtgagg aatgataaaa tatgagcaat ctaagtatat taataaaaac taatataata
     8821 aatgaattta aattaaatgc attaaaaaaa tctgatggta gagaaaaaca aaaaataatg
     8881 ggaatgatac tttcagctat tatcataaca gggttattaa ttttttatgt aaccacttta
     8941 agtctttcct tagttgatat tttaaagcaa ataaaccaga tggaaatgct actgatattt
     9001 ggatttggat tgtcaacatt aacaactatc gttacatcat tatataaaac gtcatcatat
     9061 ctttttcaag caaaagattt agacattttg actagtttac ctataaaaga atctactgta
     9121 ctagcaagta agatattaat gttggtggga gcaaactatt tattttcttc aatctgtatg
     9181 attataccag cagtggttta ctttttaaat tctgatgtaa gtataacgta tttcccttat
     9241 ttgatattgc tatttttagc tttaccatta ttaccaatag ttgcttcatc aataattttt
     9301 atgtttatag gacagatatc atcaagaata aaatataaaa atttagtatt aattataggt
     9361 agtattatat tgatgttgct ttatactcta cttatgtcaa gacttaactc tatgtcagtt
     9421 gaaatgttaa agaatagtgt atctatttca gagacgatta ggaaaatata tccacctata
     9481 tattattttg ttgatgctct taaaacagga agtgtagttt cctttttagc atttatagca
     9541 atttcaataa taccattttt tatgtttgta actttatttg caaaaaaatt taaaaatata
     9601 aatgctaaga tgagtgaagg atataagtca aaaacttaca aatttaagag tcaaaaaact
     9661 tcaagacctt caaaagctct attaaaaaaa gaatttaaga gatatacttc ttcatttata
     9721 tattttttaa attcatctgc aagtttgtta tttctaatag taggtgcaat tctaatattg
     9781 ttatttggag cagataaaat gggtgagttg ctgaaattga atatagattt taattttata
     9841 aaaatacaat tattgggagt attcttcttt ataatagtaa tgaattgtac aacatactgt
     9901 tcaatttctc ttgaaggaga aaatttatgg atactgaaat cttctccaat agatgaaaga
     9961 gaaatattct ggtcaaagat attgatgaat tttttattga atgcaccatt atctatttta
    10021 tgtttaatat tagtatctat aagattagac tttgaaattc agtttattat tgtatcaatt
    10081 tgtttgattg taagtttttc aatgtttgta gcattgatgg gattattatc aaatttacta
    10141 tatccaaacc ttgattggaa aaatgaagta gctgtagtaa aaagaagtgc aagtatggta
    10201 attacaatgt tagtatcatt agcttatgtt gttatacttg gaggtatata tacattactt
    10261 ggaatttcgt ttttaaatat atatattatt ttggcaagta tgcttacctt gatattagat
    10321 tttatgatat ggagaattat ttctactaaa ggagtaaaaa tatttaaaaa tctataggtg
    10381 tagttatgtc aaataaagac ttatcttttt tagtcttgca aataataggt taagtagtat
    10441 attatgtata gtgatatgaa aaaatttagg agaaaataaa tgagaaaatt atccaaaaaa
    10501 gaaaaagatt ttattcaata tggattttta ttatttttga tatgtattac tacttattta
    10561 gtattaacta ctttagatat aaaaatgata cctaaaatta taagactagt aaataagact
    10621 tatatatttc ttggaatatt gataattata atatatatga taattgaatc tattgtaact
    10681 catttgataa taaatgcagt tcaaaaaaca aaagtgaaat tcattggtat taaaatggct
    10741 acaatgggat tttattataa cttagttact ccatttgcat ctgggagtca accaatgcaa
    10801 atatatgcac ttacaaaata tgatgttagt cttagtaaat cagtggctat agttactaat
    10861 aaaacagtag tatttcaaag cacagttacc gtattttgtg gaattttaat tttgttaaat
    10921 aagtcattac tgatagagca agtacattct gtgaaagtct tgataacttt tggaatgatt
    10981 atgaatgtaa gtatgttgtt aggtggacta ctaatagtat ttagtccaaa acaagtaaaa
    11041 atgatagcag aagttttgat taatttttta tctaaattta aaacctttaa atttttagat
    11101 tcaaaacgtg atagaataaa tcattatatt gatgattata attattctat aaaaatgttt
    11161 attaaaaatg taagaatctt agttttaagt atattactta ctgttttaca attaatcgca
    11221 tacttcagca tagtttattg tgtgtataaa gcttcaaatt taagaggagt ctcatatata
    11281 catataatga cactacaggt ctttttatat atggctatat cgccaattcc aaccccagga
    11341 aatgttggag ctaatgaggt tacctttttt actatgtttt ctagtataat tccaaaagaa
    11401 ttattggggt actccgtgct cttatatagt gggtttgtgt actattttat attaatagtt
    11461 tcaggtatat ttacatttgg aacgcattac acattaaatt cttggaaaaa ggaaactaaa
    11521 gtaaattcta atttagataa ggttattgaa aaattaaatt aataaaataa ttagggaaat
    11581 tctatatata aatatggtgt tagataagct atattatatt gagatatttc ccttttttga
    11641 ttaaaattat agaaatttga aataataagt aataaacaga acgattgata ttgttttgaa
    11701 aggagaagtt atgaaacaag ataaaaaaaa tgaaaaacaa agtgagattt ctaagataaa
    11761 acaaagtaat gataataaac ttgaagtagc tcaagaatta catgtaagta aaaatatagg
    11821 aaatatgctt ggatatagaa tagataagaa aaaaatgaat agaccaagtt aatgaaaatg
    11881 ttgaaaatag tacattttaa aaaaaatata taatgaaatg ttttattgtt aaatttaaac
    11941 ttattttaat aagggaaaaa gtcgaaaaat atctaaaaag atatggatat aaaaaatatt
    12001 aatatatata gactataaac ttgacaaaat caacctttta atgaatagca aaaaatggaa
    12061 aaattatata agcgttattt aattatcaaa aatgaaagct aaattgcatg tgctaaaaaa
    12121 aatgattgat aacatttatt tgtgtgctat aattatatag taaaacgaat aagtatattg
    12181 cataatgtat gcaaattggg aggagagaca gacatggaag catctataca cacaattgcc
    12241 agcaataatt tattgataat ttttgctatt gtaagtataa ctggaataat ttgtagtaaa
    12301 cttagtgaaa taataaaagt tccagatgta gtgttatatt tacttgtagg tattttaata
    12361 ggaccatcat ttttaaaatt tatagatatt cgtggatttc aaatagaaaa tcaactaata
    12421 cttacttttg gttctgcatt tattttatat ttaggtggaa aagaaataag tttaaaagta
    12481 ttgagaaatg tgaagatatc tgtattctta ttagctactt taggagttgt gatatctgct
    12541 tttataatgc agcaagtgat aggatttact tttgggataa gtgcaatgac tgctttatta
    12601 gcaggaacta taatagcatc aacagaccca gctactcttg tacctatatt taatcaggta
    12661 aagataaagg atagagtaaa gcaaactgta ataagtgaat cagcttttaa tgatgcaaca
    12721 ggagctatac taacatcagc agtgttggct gtaattttgt caaataaatt ttccttaggt
    12781 gaaaatattt atgaattggg gacaatggtt attgtcggag ttttagttgg tttaattaca
    12841 ggaatactgc ttttgaaatt agttaatgat aaaccttatg gaatatttaa agattttgca
    12901 ccaataatat ctgttatttc agttgtagtt gcatatgaat tatctacaaa attaggtgga
    12961 agtggatata tgtcttgttt tattgtaggt ataataactg gtaataagaa aaactttaaa
    13021 atttggttaa gtcaaaaatc ttatgatgca gatttttatg ttgctgaaac tttaggtact
    13081 atttgtcgta tggcaatatt tataatactg ggtagccaag ttaatttagc tcagttaagt
    13141 aaatatttta tgccctctgt aattacggta ttagtattaa tgtttatagc aagaccactt
    13201 tgcgttttag tttgtacact tgtagataga gaagcaaaat ggagtaaaag tgaaatatta
    13261 tttatgatgt gggtaaggga aacaggcgta ataccagcag cactttgtgg tattatttca
    13321 gcaatgaaag ttcctggata cgaagtaatt tcatctgtag tatttatgac catactaatt
    13381 accttagtaa tacaaggaag tactacaaaa ttagtagcta aaaaacttgg attattagaa
    13441 gaagaagtta taaatataag tgaaaaagta tcaacttttt aaagttttga tttaaataat
    13501 acctattaat ggctatttta atattgtcta atgatgatta ttgaaatagt cattttttta
    13561 tgtataagca atttacagaa tttacatatt agacatattt attatcaaaa tttgttgaag
    13621 ctatgatata attagttgta tttaaaataa gaatagcaaa taagggagga aatttatgtt
    13681 tacggttatg gatattgtac agccagatac agtagaagaa gcgtactcaa ttttaaataa
    13741 aagaaaaact aaccaagtaa ttggtggaag tgctttttta agaatgggga aaaaaagaat
    13801 aggaacagga atagaattat ctaacttaaa tttagattat ataaaagaag atgaagattg
    13861 tgttgagatt ggttctatga ctacttttag aacattagaa acaagttcca taataaaaaa
    13921 taattttgga agaataatag aagattcagt aaaaaatata ataggagttc agtttagaaa
    13981 tgtagtaaca gttggtgcaa cagtgttttc taaatatggt ttttctgact tgatagtagc
    14041 attactttca ttagatacag aagtagaatt atataatatt ggaagaatta gtcttgaaga
    14101 gtttctaaat agggattatg agaaagattt acttattaag atatacataa agaagacaaa
    14161 taaaaatgct tcttataaat cacttagaaa tgcaaagagt gattatccaa tcttaaatgt
    14221 atctgtatca aaacatatga aacaatttaa gttatgtgta ggagcaagac ctcagaaagc
    14281 tacgattgca aaacaagcta gtgaatttct atctaataat gaaatcaatg aaattaatat
    14341 agataaagca gtagaaatag catcagaaga attaactttt ggttctaaca tgagagcatc
    14401 tagggattat agaaaagcta tgagtaaagt acttttaaaa agagctataa tggaggttat
    14461 ttaatgttag tgaagattaa tataaatggg aagaaaaaaa gtgttgatat agaaccagaa
    14521 gaatatctgg ttgatacttt aagaaaagct ggtaatttaa gtgtaaaaag aggttgtgac
    14581 acaggctgtt gtggtctctg tacagtttta attgacaaga aacctacatt atcttgtgca
    14641 acactgacat tgagagtttt agatagagaa ataactacaa ttgaaggttt agaaaatgaa
    14701 gttaaagaat ttagtgaagt tttggtaaaa gagggagctg agcaatgtgg tttctgttct
    14761 cctggtttta tacttactgt aattgcaatg aaggatgaac ttgaaaatcc tacagatgaa
    14821 gaaataaagc attatcttac aggaaatcta tgcagatgca cagggtatat gggacagtta
    14881 agagctatca aaacttatct ggaggtaaaa tagagtatga atatagttgg aaaaggaatc
    14941 aagaaaatag atggaatgag tatcacaaca ggaaagcctg tatatacaga tgatttatca
    15001 gcaaaagatg ctttggtagt aaagatactt agaagtcctc acgcttatgc aaaaataaaa
    15061 gatatagata catcaaaggc agaaatggtt gatggtgttg aatgtgtttt aacatataaa
    15121 gatgtaccaa aatctagatt tacacttgct ggtcagacat atccagagcc atctccatat
    15181 gacagattaa ttcttgaaga tgttgtgaga tatgtaggtg atgaagtagc tatagtggca
    15241 gctgtagatg aaaaaactgc aattaaagca atgaatctta ttaaagtaaa gtatgaagta
    15301 ctcacacctg ttttggattt tgaagaggca atagggaata acactgtagt acatgatgaa
    15361 gatagacatt gtaattttga tataggtatg caaagagaaa aaaatatagt atcacatcat
    15421 gattatacaa aaggtgatgt ggaaaaagta ttaagtaatt gtgatatagt gattgaagaa
    15481 acttattata ctcaagctca agcacatgca atgatggaaa cgtatagagc atataattat
    15541 ttagaccata ctggaagatt ggttgtagta agttcaactc aaataccatt tcatgtaaaa
    15601 agacatttat ctcgtgcact agaactacca tctagtaaaa ttagggttat aaaacctaga
    15661 attggtggag gatttggtgg taaacaaact gcatgtgttg agattttttc tgcaattgtt
    15721 actttaaaaa cagggaaacc agctaaaata atttatgata gaaaagaaac ttttaattgt
    15781 tcaacaagta gacatgcaat gagattaaaa gttagactag gtgctacaaa tgatgggata
    15841 ataaaggcaa tagatataaa agcactttct gatactggag catatggaga gcatgcttct
    15901 acaactattg gtttggttgg agaaaaaact ttaccaatgt ataataaagt tgaagcaaca
    15961 agatttattg cagatgtagt atatactaat aagatgccag caggagcact tagagggtat
    16021 ggagcatcac aagggtgttt tgctgttgaa tcaactataa atatgcttgc taataaatta
    16081 aaaatagacc caacagattt aaggttaaaa aacgtggtaa cagaaggtga aactacattt
    16141 gcatatggga aaactttaaa tagtgtcgac ttaataaaat gtataaataa aggcaaagaa
    16201 cttattggat ggaatgaaaa atatccttgc aaatatcttg ataatggaaa agttagaagt
    16261 gtaggtatgg caattactat gcaaggttct ggtatacaag gtcttgataa gtcttcagct
    16321 gaaataaaat tgaatgactg tggagattat actcttatga taggttctac tgatatgggt
    16381 acaggaagtg atacaatttt agctcaaatg gctgctgagg ttcttgagac gagtattgaa
    16441 aatataacag ttatatcagc tgatactgac ttagttccat atgacccagg ttcatatgct
    16501 tcaagtacta cttatgtaac aggtatggca gtagttaagg cagctcaaga tttaagagaa
    16561 aaaataattt ctgctggagc aaataaacta ggtgttgata aggatgaagt agaatttgat
    16621 ggtataaaat taactagtaa aaaaggtagt actttattat ttgatatagc agtggattct
    16681 acaattggtc cagatactaa tcaattaata ggtcttgctt ctcatggtag ccctgtatct
    16741 ccaccacctt ttatagcagg ttttgcagaa actgaaattg acaaagaaac tggtaaagtt
    16801 gatttagtaa attatgtagc tgtagtagat tgcggaacag ttataaataa aaatctagct
    16861 aagatacaag ttgaaggtgg aattgttcaa gggattggaa tggcattatt tgaagaagtt
    16921 agatacacag aaaaaggaca catggataca aatacattta tgcaatacaa gattccagct
    16981 agatgcgata ttggaaatgt taaagtagat tttgttgaaa cttatgaacc aactgggcca
    17041 tttggtgcta aatctgttgg agaagttgtt ataaatacac ctttaccagc aatacaagaa
    17101 gctgtgttta atggaattgg tgtaagaata aatacacttc ctattacacc tgaaaaagtt
    17161 tttatggcaa tgaataaata attatttatg aaattttaga ctagttttac atgttaaaca
    17221 accagttatg tcatacctgt tgaaagatat ttaataagta gttattataa tattgtgaat
    17281 tttacttata aaatacaaaa ttaaataagg ggatgatgtt ttggctggaa atctaaataa
    17341 tatgagagca gtaaataatt ttagaggaga taagaacatt ttagaatgtt tagtcagctt
    17401 tgagggtcgt tcaataagtc agagaaaagc aaaggtattt tttaaagaaa aacaaaatca
    17461 aatagaaatt gattttgtgg aagaagaaat ttctaaattg attgaaaatg ttgttttgaa
    17521 tacatcatat caggaaatgt tatatgatga aatagaaaaa caactggaaa ttgattgtat
    17581 aggtacttgg atgatattat ctaaattgaa agatggtaga gttcattgat tttaaaataa
    17641 attattagag cctaatttta aggctctttt ttactgcttt taattaacaa cagaaaatct
    17701 atatttaatt tacatataat tttaatatca tatattctat atatataaaa ttgagtttgt
    17761 atgtattata ttgtataata taaattgaat tagtgaaata gttcgttaaa ataatttgtt
    17821 tgtataaaat cattgtttaa gtttatagat tttgtataaa ataaagttat gttattaatt
    17881 tttttagaag taaaattttt actaggagac agatatgaga aaaaataata ttaatatagc
    17941 attaacctca gcaattacag caatctatgc agttttaaca ttatcattgg gatttataag
    18001 ttatggacca atacaattta gggtagcaga gattatgatg cttttggcat ttttagataa
    18061 aggatatata gtaggtctta cattaggttg ttttttagca aatgtaatag ggccatatgg
    18121 tgtgcctgat ataatttttg gaacattagc cacatttata agtgcaagta tgatttatat
    18181 tactagaaag ttaggtaagc aaaataaaag tactttaatt atagcttctt tatggccgac
    18241 tatcataaat tcgattgtgg ttgggctaat gttaaatata ttctttggac ttccgttaat
    18301 tttatctatg atacaggtag gttttggaga gtttgtagta gtaacaattg taggagtacc
    18361 atttttttgt tttttaatga aaaaatataa aaatattatc aatattaaat tttaaatagt
    18421 gtatagtaat ttaattgaga gtattattgg tgaaaagagg tgtttatttg tcaaaaataa
    18481 aaagattgac tgatgtttgg acatatatta atgataagca aaagtttaca ataaaagaat
    18541 tatcagagga atttaatctt tcagctaaaa caatacaacg atatttaaaa gaattaaata
    18601 aaatgggact tccaattcaa gcagaacaag gtagaaatgg tggttatagg gtgctaaata
    18661 atagctatat acctcctgtt atatttacag aaaaagaagt tatggctatg ctatttgcac
    18721 ttaagtcact tcaggtttat aattataagt caattgagat agaaattaat tctataatga
    18781 gaaaagtaag ttatgagcgt aagagtagta taaaagtagg tatagaaaat attaaaagat
    18841 atattggatt tgtagctaat aattctaatg atggagagtt taagagtatt aatgtaactg
    18901 agttttttag agcttcttca gagtctctaa ttttaaatat taagtataag tttggtaatc
    18961 agattttgga aaaaaaaata tgtcctatag gtatatattt aaataaagga gcatggtttt
    19021 caccagccta cgattgtaaa attgataaaa taatattagt tcatatagat aatgtaattg
    19081 acataaaaag aacaggagaa agtaaaaaaa tacatataaa cttggaagag tggctagatg
    19141 aagtatataa caatttatat gaaaataggt caaccactaa aaaaattggt gaaacgattt
    19201 atctaaatgt attattaact aaagaaggtg tttctaaaat aaaggacagt tcttataatt
    19261 tagatatgga actaaatgag gatggtagtg gaacaattag tacatttata agggttgaag
    19321 atgtagatta ctatgcttct attttatttt taattggaaa taatgctaaa gttatagaac
    19381 caaaatttct gaataagcta ctttatgaca aggccaatga gttaagatat ttttatgaag
    19441 aaaatatggt ataatattta gaaatgttga aattaaataa ttattaagga gatatacatt
    19501 ataatggaaa attttaataa gaatgctaaa ataattggta ttggagctga aggtatcaat
    19561 ataataaatg aaatagaaga aaaaataaaa gccaatatgg atatagaaaa aataaacata
    19621 aatcaagaga ttgaaaaaga atacgttaga agtcttctag atggtgtaga tatattattt
    19681 ttaatatatt catctgaaga taaacatatt aaagagatta taaaagctat ttcatatatg
    19741 tcaaatgaga gaagagttct atctatagga atggattgtt cagagaaaga aaataaagaa
    19801 gctttagagc ttggaagaga atttaaaatc aataacgata gtatatttaa atttgttaat
    19861 ttaatgaata tcatggtaga gtctatttct gattcttgca tgataaatat agatattact
    19921 gatttaaaag aagctattgt aggagataaa ggaattaagt attcttttga agagtttgaa
    19981 gatacaaagg aatatagcga gatggctgac attttatttg acagaatgga atatattggt
    20041 gaggaattta taagcaagaa aggaattgta tttgttgaag gaagctcgga attttctata
    20101 atggaactta atgatttaat aaataatatt cagagaaaga tagaagaaag ttatgaagta
    20161 atattttctt tatatatgaa ggaaaatcta gatggaaata ttagaatagg tttattgtat
    20221 aattagtata ttttagatat taaggagagt gtatatgtct gaaaatataa gagttacaga
    20281 gtactggcta atggaagaat ttttttatca ggaaacagac acaccacaga tttttagaga
    20341 agaaagtata attgcacttg gatttgatat aaaaataaat ttaaaaaaag tatttggtat
    20401 gggaaaaaaa tcagaaaagt atattaatga gaattgtaat gataaagatt ttataaaaag
    20461 atttgtgtca attaaaaaag gagattattt tttatataag gcatatcaaa atatagaaga
    20521 aaaagttaaa tgtaatgtgt ctattggcat ggttgaagaa gattttgaaa atggatatga
    20581 attaagtcat attttagggc atacactacc agtaaaatgg atgtataatt ttgatacagt
    20641 ttcaaattgt aaagtatata caagtgaatt ttataaatta aataataatg atattagctc
    20701 atatttagaa attattaatt cacaagataa gaaatcattg agaaaaagaa tagaagaaag
    20761 tgaaaataat aaatggtatt taaaaaacta ttattataaa aatgtagata aatgtgtaaa
    20821 tgatgaagaa ctatttattc agcctactaa tctagaacag ttagaaaata taaaaaatat
    20881 aaaaacagat gattacattt tattgtataa tagcgagaaa gtaatctgtg aaaatgacca
    20941 tacaatgaag agtctatttg gtgtatgtca agtcatggaa ggattcgata aagagaaagt
    21001 aagtactttg gaggatggat attttttccc tgttagatgg atagagtatg aaaaggaaaa
    21061 tatagatgga aatgcatggg ttatatttga aaataatagc atggttgaaa agtatgtaaa
    21121 aagatttact aataaaatac cattaaatac tatactttat ggaccaccag gaactggtaa
    21181 aacatacaat acaaaacaga tggtttataa tctagtaaat aaaactagtt taagaaacag
    21241 gtactataca ggttacttag attataaaaa taatgtagaa ttttgcacat ttcatcaatc
    21301 atatggatat gaggaattta tagagggtct taagtctgat ggagaaggca actttaaacc
    21361 agaagatgga atattaaagg atatatctat agaagcatgt tatgatgcat tgaaatttga
    21421 aaaaaagatg agttttgagt tagaaaaaga tagcttgact ttagaagcta taaaaaagag
    21481 aaaaaagaag ttagtattag aaaatataga atatagtgat agttttaatt ttgaagaatc
    21541 agaaaattat gttttaataa tagatgaaat taatagaggg aatgtgtcta aaatatttgg
    21601 tgaactaata acattattgg aagatgataa aagacttact aaatcaaatc agactattgt
    21661 caaactacca tattcaaaag aaaaattttt tctacctccc aatttgtata tagttggaac
    21721 catgaacaca tcagataaat caatagcact tttagatata gctttaagaa gaaggtttaa
    21781 ttttgaagaa attatgccaa attatgattt attgtctatt atagatggca tagatattag
    21841 aaaaatgcta tatacaatca ataaaaggat agagtttttg tatgatagag accatgttat
    21901 tggacaagcc tatctacttg ctgtggaaaa tattaatgat atagtagtag tatttagaag
    21961 taaaataata ccacttcttc aagagtattt ttattatgat tcagaaaaag ttggtttgat
    22021 tttaggagga attggaagaa atgaaaaaga taaatacatt gtttataaag aagaacttat
    22081 agcttctgag ttatttaaaa ataataatgc ctcaataatg gagagtaaag ttaactattg
    22141 tataaaacaa aacatatcta gtgaggagtt aaaaaatata tatgagtaaa aatatatatg
    22201 ttaaggaaac ttatgaatgg ataagagtag gtaatggtga aaatgagctt acagaaattg
    22261 aatatgaaaa attgcttaaa tatctagaga acaataatga tgtattaaaa agtaatataa
    22321 tagatattaa atacaaaaaa cttagattta taaactatgt aggtattata tgttttgaaa
    22381 atataatact tgaaatactt cctaagttgt cattaagtga taatcttgta aaagatagag
    22441 agatactatt acagatgctt tccatatgta ataaaattcc aattataatg aatgaaaaaa
    22501 taagattaag tttaaaaaat tataatctcc taaatttttt tattatgtat ttcattgaga
    22561 gtatgcaaat acagatgaag aaaggtattt attttgaata tataaataaa atagagaact
    22621 taaatgttat aaggggtaaa atattattaa gtacatatgc aaaagaaaaa tgtatttctc
    22681 caatgaaaat aagatgtgaa tatgatgaat atagtgaaaa taatttcttg aatcaagttc
    22741 ttaaaaaagc atgtatatct attttatgta gaataaatga caattcaatt caaggaaaga
    22801 ttaaaaaaat tttaaactat tttcaaaatg tagatttaat ttatatagat agaaaaaaac
    22861 tattagatta taaattttgt aaaaacaatg atagatttag agattgttat ttactagcta
    22921 gacttctact tttaaatttg tctatggata atagtcaaga taatgaagag gcattttcta
    22981 tattatttga aataaatact ttgtatgaag aatatatagg aattttaatc aaaagtatat
    23041 gggataattc atttagagaa acatatatac aagataagag taaatttctt ttgaaaaatg
    23101 aacaaacagg taagaaaaac tttaatttaa gaccagatat agttttatat gatttgaaaa
    23161 gtgaatatga aattataatt gatactaaat ggaaagctat tgaagtagat tcaaatgttt
    23221 tttataggtc aagtgatata taccagatgt atgcatatat aacaacatat gaaaatgcaa
    23281 aaagatgtat attattatat ccttgtattc aaaaagataa aaattatagt tcttggaaat
    23341 tatcagaatc atttaagggt aagtttatag aagcaaaaac tgtgagatta gatgatataa
    23401 aaaacacaaa aaatgattta aaaaagataa tttttaatta taaattttaa aaataagaat
    23461 tttaaaattt ataaaagtaa ttttatatag gtataaaatt tctgagtagt ataaaaaatt
    23521 taatagagac ttagattaaa aattattttt atgtaaaata tggcatttta attgcttatt
    23581 taatagagga ttcttgtaaa gttaagaaag tatatctatc aaattaaaac taagtggata
    23641 tactttttat taagttttgt tttaaaataa tattattaaa ttatagctat gcttttctgt
    23701 tatgatataa ttaaatagat aatgtgaatg attattatat agaatactta tttggagttg
    23761 atattttatg gacaaacaca gtgattcagt aatgtatggt ggaatggaag tatacaatat
    23821 ttggggaaca acaacagtag actccataat tggagagtct gatgtaatgc gtgctcttaa
    23881 gaatagaatt tgcaaaattg gaaatagtga ttctactgta gctataactg gagaaagtgg
    23941 cactgggaaa gaactaatag caagagccat tcatctcgca ggaaacagag ggaataaaac
    24001 attcgttgct ataaactgtg ctgctatacc agaaccttta ctagaaagtg aactttttgg
    24061 atatacaaaa ggagctttta gtggagcaga ttcaaaaggt aaaattggta agtttgaatt
    24121 ggccaatgga ggggttgtat ttttagatga gataggagat atgccaattc aactacaatc
    24181 aaagttattg agggtattac aggaaaagaa gtttgctaga ataggttcaa atgaactaat
    24241 tgatatagat gttagaatca tatcagctac taatagagat ttatttgagt tggttaagga
    24301 aaataaattt agagaagact tatactatag attgaatgta atacctttag aggtgcctcc
    24361 acttagagaa agaggagacg atatacaaat attaataaac aattttatag acaaatattc
    24421 gagaaagatg aagaaaaacg tgtaccatat agaaccagat gtggaacaaa tgctgcttaa
    24481 gtatccttgg ccagggaata ttagagagtt ggagaatact atagaacttg ccttaaatct
    24541 tcttgaggaa gatggaataa tacataaagg tataataaac agaaagataa ttgaatattt
    24601 taagctaaat aatataaata taaagctact tgaggaaaat gactatgaga taagtatgga
    24661 acaagacata cttacgcttg aagaatttga aagagcctat ataaagaaag tgttaaagtt
    24721 ttacggaaat gatactaagt ctaaaaaact ggctgctaaa aaacttggaa tatcactagc
    24781 tacattgtac agaaaaatag atgtataata attagacttt ttattctcaa ttctaaaatt
    24841 tgaaattata aaattgataa aaaacacttg gaaaactatt ttctaagtgt ttttttgtgc
    24901 aatagtattt tattatccaa aaataaaaaa aatttgaaag aatttaaaaa aagaaatgct
    24961 ttaataaatt atcaaatact aacaaatata ataaaaatta tatatgtaaa acaattatgt
    25021 taaaaaattg gcattataat tgcttcatat taagttgaga tgacaaaata aaaaaagaaa
    25081 ggatgttgtg tgagataata ataactgtca ttaaaaaata gttgggagtg ataaccttga
    25141 gtaaatatca aaaaaatgtt tctatgtttg atattgatgg aaggccacca attttaaagt
    25201 gtttaccact atcaatgcaa catatattgg caatgatagt tggaactgtt acagtaccta
    25261 tagtcgtagg aggagctgtg ggtgttagcc ctacacaaat aacactttta gtgcaatatg
    25321 cattaataat agctggtata tcaacactta tacaactata tccaatagga aatattggtt
    25381 caagattgcc tataattttt ggagttggtt ttacttatgt accaacactt gtagcagtcg
    25441 gaggaaatta tggattagca agcattttgg gtgcgcaatt aataggggga gtggctacag
    25501 tattaatagg gatatttata aaaaagataa gaaaattttt tccgaatata gttgcaggaa
    25561 cagttgtttt tacaattgga ttatctttgt atcctatagc aataaattat atggcaggtg
    25621 gtgtaaatag tcctacatat gcttctatgc aaaactggtc agtagcggct ataacactag
    25681 tggtagttat aggtcttagt caatttgcaa aagggtatgc aaaattggcg gcaatatttt
    25741 tgggaattat agttggatat tgtgtatctt tagttttggg tataataaat tttgacccta
    25801 ttagagaggc aacttggttt gcgataccta aaccacttga atttgggatg gaatttgata
    25861 tgcctgtaat aatttcagta gtaattgtaa gtataatcaa cgctgttcaa gcaattggag
    25921 atttatcagc tactacaatg ggaggtatgg ataggaatat cacagacaag gaattatcaa
    25981 gtggagtaat aggaagtgga atttgtacta tgataggagc tttgtttgga ggtttgccac
    26041 catcatcata tagccaaaat gtggggatgg tagctatgaa caaagtaatt agtagatttg
    26101 tgcttggaat agctggtata tttatgttat tgtcggggtt tgtgcctaaa tttggtgcat
    26161 taatgactac cataccatat tctgttttgg gaggagctac aatatctata tttagtatga
    26221 taacaatgac aggtataaag ttaattatta cagaagagtt gtcatcaaga aatattacca
    26281 ttgttggttt ggcaattgct ttaggcatgg gaataacatc agtcccagca gcccaagaag
    26341 cgtttccttc atttgtaaag ttagtatttg gagaatctcc aatagtaatt gcgacattgg
    26401 ttgcattcgt tttaaatcta ataataccaa ataaaagtct agaggatgaa gctaaagaaa
    26461 gagctgttat ggaagagact aattaagata taatagtttg aaaggattgt tcaaattatt
    26521 tagtatgaga ttgttagatt atttttgata gctattcatt ttaataaaaa atttaacaaa
    26581 taaatatata ccaaataatt aatatttatt taagttgaaa atcggggagg taatatttag
    26641 aaatttgtta attttttata aaaatagctc agtttagctg tgtttatcgg ttgagcaata
    26701 aattcatggt cataaaaata tttatagaaa ataattataa aaatatttag tcaagatatt
    26761 tatgtatggg aggatttatt ttatgaaaac acgtaatact tcaaaatatg atgtcgatgg
    26821 gataccacca ctaaaggaat caataccttt agccttacaa catctgttag caatgatagt
    26881 aggaaatatg gtacctgcga tattaatagc aaacgtggta ggacttaacc aggggcaagc
    26941 aacaatgttg attcagggtt ctatgttagc agcaggtttg gcaacgtttt tacaattata
    27001 tccaatacct ttatttaaag gctttaaatt aggttcaaga ttacctgtaa tgatgggaat
    27061 gagttatgta tttttaggag cctgtctttc agtagccgct aaagatggtt tagctgctct
    27121 atttggagca cagattgctg ctggggtaat tgtattcttt gtaggttttg gagttaagaa
    27181 aattagacat atattcactc caatagtttc tggaactata atagcatgta tgggacttgg
    27241 attatttgca acagctataa agaatttagc tggaggagaa ggtgcacaaa catttggaag
    27301 cccaataaac tttacagtag gagttatagt tgcttttgta ataataatga taaataaata
    27361 tggtaaaggt ttagtaaaaa attcttcaat attaatagga atattagtag gttatgctat
    27421 atctttagtt ttaggattgg ttgatttctc agccgttcag ggagcagcta tagtatcatt
    27481 accaacacca gcagcttttg gattagaatt tagaccagag ttaatagtaa tgtttactat
    27541 tatttacata ataggtatag ctgatatgat gggtgcctgt acaatagcta cagtaggagc
    27601 aatggataga gaggtaactg atgaagaatt agcatcagtt gttttaggaa actctgtaac
    27661 ttctataatt tcatcactat ttgcagctct tccaactgga gtatttagcc aaaatacagt
    27721 aattgtatct atgaacaaag ttacaagtag atttgtaata gcactagggg cacttgtatt
    27781 attactagcg ggaatatctc cagcattggg cgcaataatg acaactatac catcttgtgt
    27841 tgttggtgga gcaactttag tagttttctc atcaatagca atgtctggtt tctctattat
    27901 gagcatggat ggatttactg aagaaaacaa tcttatagca ggtgtatcta tagcaacaag
    27961 tatgggtctt acgactgctc cacaagtttt agaccaattc ccagaaacaa ttggaactgt
    28021 ccttggaggg tcaagtatag tttctggagc aataatagct atattacttc agacactatt
    28081 taaattaaag tcacgcaaaa gtgctgaaaa cgttacttca aatcttgagg aaaatattgg
    28141 ataaatgtat tagatataat atgagaaaac catatggtaa aatgttgtaa cgaaagtatt
    28201 caaaggggga tttaagatga ttgatatact aataaaaaat gcaataattg ttacagttaa
    28261 taaagacaga gatgttatat ttgatggagc aatagctatt caaggtgaca gaatcttgga
    28321 tataggaaat acagttgaca ttgaacctaa gtatcttgat gctaaaagag ttatagatgc
    28381 taatggaaaa gtaatattcc ctggctttat aaatactcat aatcacttgt tccaagtatt
    28441 attaaaaggt ctaggcgatg atatggcact tcatgagtgg ttaaatacaa tgatgtttcc
    28501 atcagcaaaa ttcttaacag aacaagatac ttatgatgct gcaatgcttg gatgtatgga
    28561 aggtttaaaa agtggtataa ctacaatggt tgattatatg catactcaca atagaccagg
    28621 acttacagat ggaatagtaa aagcatataa agatttaggt ataagagggg ttgtaggtag
    28681 aggttgtata gatttaggta ttcacaaaga gcttatagaa gatgtggaaa cggttgaaaa
    28741 agatttgaga agagtatttg aaaaatatca taattcagaa aatggaagaa taaaaatctg
    28801 tgtagctcct tcttcaatgt gggctatatc tgaagaaatg ggtaagatgc tttggaatat
    28861 agttaaagaa tatgattcat attttacagt tcatatatct gaaacagaat ttgcaagaac
    28921 agcaacaaag gatatacatg gagaaataga tataaaatta cttgaaaaat ggggcatagt
    28981 aggtccagaa gtcgtagcag tacattgtgt gtgcataact gatgaagata tggaaatgct
    29041 taaaaagtat gatataaaag tttctcacaa tgtagcaagt aatatgtatc ttgcttctgg
    29101 tgttgctcct gttccagaaa tgttaaaaaa aggtataaat gtgagtttag gtcttgatgg
    29161 tgcagcaagt aataatgcac aggacatgat agagcttatg aaactaacag ctttacaaca
    29221 caaagtaaat aattgtgacc ctctagcaat ttctgcagag aaagttcttg aaatggctac
    29281 aatcgaggga gcacgaactc ttagaatgga agatgaaata ggctctttag agattgggaa
    29341 aaaagctgac ttagtaatat ttaactcaat gctttctcca aaatcaatac ctcttcacaa
    29401 tcctgtatca actttggtat actcagctag tatgcacaat gtagagagcg ttttagttga
    29461 tggaaatata atacttgaaa atggaaaagt aactacaatt gaaagtgaag aacaagtgtt
    29521 gcttaatgca caacatgcag cagaagaatt atgtaagaga gctaatgtaa ctaatagaat
    29581 ggaaggacat aagtggaata gcttatacta atattaaaat aagattgtaa taatattcta
    29641 ttacaacatt agtttatctg taggaggaga aaatggaaaa gataaaatta aatattaatg
    29701 attacatgta tgaaatagaa gttgaaaaga atgaaactct tttgcatgta cttcgtgaaa
    29761 aattaaatct aactggagcg aaacaaggtt gtaatagtgg cagttgtgga gcatgtaaag
    29821 ttttgataga tggagttgac atgaaatctt gtaaacttct tgcaagaaac tgcgaaggta
    29881 aagttatata cactatagaa agttttgcta atggagaaaa tcttcatcca atacaagaag
    29941 cttttataga agttggagct gtacaatgtg gatattgtac tcctggtatg attataacaa
    30001 cacaagctct tttaaataga aatgcatctc ctaatgaaga tgaaattaga gaagccttta
    30061 aagagaatct ctgtagatgt acaggatatg taaaaattat agaagctgta aaattatctg
    30121 cagctagatt agggggtaga taattatgga aaaagtaata ggaaaatcat accctctaaa
    30181 agatgcagct ttaaaagtta ctggtcaaat gaagtatgtg gctgatatga aaccacaaaa
    30241 tgtactttat gctaaaatgc ttttaagccc tgtagcacat gcaaaaataa aaagtataga
    30301 tacaagtgaa gctgagaaag tagtaggtgt tagagctata gctacttatc taaatacatc
    30361 acaaagaaaa tacaatggtg cattaagatt ttatgagcac aatatacctg aaaatgaagt
    30421 tattttttca gatacagtta gatttgttgg tgaccgcgtt gcagcagtag ccgcagagac
    30481 tccagaaata gcagagaaag ctataaaact tataaaagtt gaatatgaag agttaccagc
    30541 tgtatttact atacaagagg caataaaaga agatgctatc tgcatacatg gagattcaaa
    30601 tatagtttct caaaataaca taaatggggg tgatgtagaa aaaggcttca aggaatgtga
    30661 ctatatattt gaagatgaat actctactcc agcaatacat catagtgcaa tagagactca
    30721 tagtgtaatt gcacactatg attataatga taaattaact gtacatacac cttgccaaaa
    30781 tacatttgga tttagaataa tactgtctca aatttttggg atgtcattaa ataaagtaag
    30841 agttataaga cctgctattg gaggttcatt tggaggaaaa ttagaactta caattgagcc
    30901 agtagcagcc gcattatcaa aaatgacaag aagaccagtt aaaatggttc ttacaagaaa
    30961 ggaatctatg atatcaacaa gaactcgtca tgggtccttt gtaaaattaa aaactggtgt
    31021 aaataaagat ggcgaagtaa tagcacaaga tataaaaata tatacaaata caggcgcata
    31081 tgcatcaagt gctttaaatg tcataggagc acttagtcat aaagtattta aagtttataa
    31141 aataccaaat ataaagttta ctggaatgcc tgtatatact aatacaccaa tagcaggagc
    31201 tatgagaggt tatgggtcac ctcaaatatt tatggctcaa caggctcaat ttgcgaaaat
    31261 agccaaggaa ctaggtatgg atttagtcga ttttcaaaat aaaaatgcag tagagccaga
    31321 tgatgtcgat ataatcttcc acggttctct tggaaatcct agagtattgg attgtataga
    31381 gcaaggtaag aggatgttta agtgggatga aaagaaaaaa cttccaaaag aagaaggtaa
    31441 atatttaaga ggtataggta tggcaatagg tgcacatggt aatggagttt ttggtgcaca
    31501 tagagatgtt gttacattga ctcttaaatt aaatgaagat ggtacattga cactaataac
    31561 aggagcacat gatatgggta atggtgttgt aactatgcaa acaatgatgg tagctgaaat
    31621 tttaggaata acacctgata aagttgatac atttgaaact gatacggatg catgtacatt
    31681 caaccttggt gattatgcta gtagaggggt atttgtagaa ggtggaggag ctaaaaagac
    31741 tgcagaaaaa ttgaaagtcc ttatattaga agaagcagaa aaattacttg aaacatcaaa
    31801 agaagattta tatctagaca atggatacgt aattagtaaa cttaatgaaa atataaaagc
    31861 atcactttct gatgtcgcag tatatagtca aagtaaatca ttaagagagt taactgttgt
    31921 tgaagattat tcttcaccaa ctggacttac ttcttatggt gtacattttg ctgaagtttt
    31981 agtagataaa gaaactaaag atataaaagt agttgatttt gtagctgtac atgatgttgg
    32041 tagagttata aatcctctaa atttagaagg tcaattagaa ggcgggattc atatgggtct
    32101 aggatacgca ttgagtgagg agttggcatt tgatgaacaa ggtagaccta aagctactga
    32161 ttttaaaaca tataaattat ttagaactgt agatatgcca aaatgtcaag ttgcatttgt
    32221 gaatagttat gaaaaagctg ggccttttgg tggtaagagt attggagaat gtgctgttgt
    32281 accagttgca ccagctgttg caaatgcagt atataatgca acggatgtag aaatacattc
    32341 attgccaata aaattaaaat agtagaaaaa gcataaataa caatttattg tatttgtaaa
    32401 tagataataa aaatagattt atttggtgag gagatattat tatggcaaag atattaggga
    32461 tttgtggaag tccacgtaaa ggagcaacag aatatgcagt gctagaagct ttgaaagaag
    32521 cagaaactat accaggtata gaaactgagt attggtcagt tagagggaaa aaaataagtc
    32581 cttgtgtaca ttgtgatgca tgtataagaa aagaaactat gtgtattata aaagatgata
    32641 tccaagaatt agagcaaaaa atattggagg cagatggaat tatagtaggt tctccagttt
    32701 atgatatgaa tataactgct caacttacag cagtgtttaa tagacttcgt ccaatgtatt
    32761 tagttcatcc aggaaaactt caaaataagg ttggagcagc tataactact ggtggaacta
    32821 gatacggtgg tcaagaattg acaaaacttc ctataataaa tttcttccta atgcatgaaa
    32881 tgttagtatc tggaggttta ggtggatgct atataggtgg tacaatatgg agtaaagatg
    32941 gaaaagcaaa aggtgcccaa gaggatgaga caggtatgga cacagtaaaa agacttggta
    33001 aaggtgctgc agaggcagtt atggtttcta agtttggtct tgaaaaatgg aatgtcgtaa
    33061 aagaagaatt agatgttaaa aaagatgaaa aatcacctct tagagaccat taaatattta
    33121 ttatattatt aaatatatac aaatagtgat gtaatagatt attgcatcac tattttatgt
    33181 tttaatttag attttataaa ttttatatta aaaaatatag aaacaaagtg ttttgagatt
    33241 tgtttttaaa ttttaaaaat gagaattaaa aagtgagaag atagaaaagg ttttttagtt
    33301 attaaattct atatacattg tatgaactaa agatagaaaa gaaaaaataa taaaagcatt
    33361 gcaatttcta tactaaagag tcatggaaaa atatttagta tatatttagt gttggcatgt
    33421 taattgctta tatattaaag tataaaaatt taacaataat ttggaggtat tttagatgtt
    33481 aaaagaaatt aaatggaaag taaataattt gccaaagggt gataaggaaa attgtatcaa
    33541 atttttaaat gaagaagaaa taactaaggt tagaaacttt cacaaaagct ttcctcaata
    33601 taaagaaact ccattagcaa acctagaagg tttagcaaaa aaattaggtg ttgcaggagt
    33661 atatgtaaaa gatgaatctt atagatttgg attaaacgca tttaaagtat taggtggctc
    33721 ttattctatg ggtaaatatt tagctcaaag attagataca gatatatctg agttagggta
    33781 tgacaaatta acttcagatg aaataaaaga aaagttagga gaaataactt tctttacagc
    33841 tacagatgga aatcatggta gaggagttgc ttggacagct aataaattag gtcaaaaatc
    33901 tgttgtatta atgccaaaag gttcttctga atttagatta aataaaatca aaggagaagg
    33961 tgcagatgct agtataacag acttaaacta tgatgatgca gtaagattag ctaatgatta
    34021 tgcagaagct gatgaccatg gagtaatggt tcaagataca gcatgggacg gatatgaaga
    34081 aataccagca tggataatgc aaggatatgg aactatggct caagaagcta tagaacaatt
    34141 aaaagaatat ggagtagata gaccaacaca cgtatttgtt caagcaggag ttggttcact
    34201 tgctggtgca gttcaagggt atgttgcttc aatatatgat gaatgcccaa taacagtagt
    34261 agtagaggct gatgaggctg attgttacta taaatcagca gaagctggag atggaaaacc
    34321 aagatttgtt ggaggagata tgccaactat aatggcaggg cttgcttgtg gagagccaaa
    34381 tactatagga tttgaagtgt taaaaaatca tgcaacagca tttgtttcag caccagattg
    34441 ggtatctgct aaaggtatga gaacattagg taatccttta aatggtgatg aaaaggtaat
    34501 ttctggagaa tctggagcag ttacaactgg tcttttagta gcagctatgg aaagagaaga
    34561 tttagcagat ttaagaaaag atttaaaatt agatgaaaat tcaagaatac ttttaataag
    34621 cacagaagga gatactgacc ctgacaaata tagaagtata gtttgggatg gagaatatcc
    34681 aagtatataa ggaattacta aaatgcaatt attagatatt ggtttacata tttagaagat
    34741 atataagttc taaattttag aaaatatata gtatataaaa tacatacatt ataaaaaatc
    34801 tagggggata atgaaatgtt aaacgaacaa agaaaacaag aagtaactga aatatgccaa
    34861 aaattaattc aaaatcctag ttcttctgga aatgaagaag gtgttgtaaa agcaatagaa
    34921 gagtcttttg aaaaattagg ttttgatagc tggtcaagag atagatatgg aaatatagta
    34981 ggatgtataa aaggtaacaa gccaggtaag aaaatattat ttgatggaca tatagataca
    35041 gttccagttc cagatgcaag taaatggagt gttcctcctt ttgaaggaaa aatagttgat
    35101 ggaaagatat atggtagagg aacttctgat atgaaaggtc aatatactgc aatgatgtct
    35161 gctgtagctt attttgctaa agatactaat aaagattttg ctggagaact atatgtagca
    35221 ggtgttgttc atgaagaaat attcgaagga gtttcagcta gagagataag taaagctgtt
    35281 caacctgact atgttgtaat aggtgaatct tcagagttaa acttaaagat tggtcaaaga
    35341 ggtagaggag aaatagtagt agagactttt ggtaaaccag ctcactctgc taatccagaa
    35401 aaaggtgtaa atgcagttta taaaatggct aatgtaatcc agaagataca acagttagta
    35461 cctccaactc atccagtttt aggtgatgga atactagttt taacagatat aaaatcttct
    35521 ccatatccag gagcttcagt agtgccagat tattgcaaag ctacttttga cagaagatta
    35581 ttagttggag aaacaagaga aggagtatta gctccaatac aagctttatt agatgaaatg
    35641 atgaaagaag acccagaatt aaatgcaaaa gtttcttatg cagttgaaaa agctgactgc
    35701 tatacaggaa atactataga aagtgaaaga ttcttcccag gttggttata tgacgaagaa
    35761 gatgagtttg ttcaagcagc gtataaggga ctaaaagaag ctggaataga ttcagaaata
    35821 actcaatact cattctgtac aaatggaagt cactatgcag gtgaggctgg aataaaaact
    35881 ataggatttg gtccttctaa ggaaaatctt gctcatacaa tagatgagta tatagagcaa
    35941 gagcaattat ttataggaac agaaggttac tatggaatat taaaatctgt atacggaaaa
    36001 taatgtttag ttcacaataa attatttaaa tatagtctag aggtgctgaa tatagcacct
    36061 ttagaaaata caattttaaa taaaatttta cgatacctag aaaatacttt aaagcttaaa
    36121 agttaggagg tatatatggg aacaatacta agaggtggaa ctataattac ctctgataag
    36181 acttatataa gtgatttaag gatagaagat gaaaatatag ttgaaatagg aaataaccta
    36241 gaaataaatg gtgataaggt tattgatgct acaggtagaa ttgtaattcc tggtggaata
    36301 gacacacata ctcattttga tatgaatgca ggttctatta caactgcaga taattttgaa
    36361 acaggaacaa gggcagctat agctggagga actactacaa tactagattt tgcagaagcc
    36421 aacgaggggg aaaatttact acaaggtgta gaagcatatc ataaaaaagc aattggaaat
    36481 tgttattgtg attatggttt tcatatgaca attacatgtt tagataaaga tacatttaaa
    36541 catatggaaa agcttatttc agatggaata gtatctttta agatgtatat ggcatatgag
    36601 ggcatgaaag tagatgatgg aacaatatac agagttttaa agaaagcaag agatttaggc
    36661 tgtatagttg gatttcattg tgaaaatggg gatttactaa atgtattgat agaagaaaat
    36721 atacaaaatg gtaatctaga gccaaaatat catccactta caagacctaa catagttgag
    36781 aaagaatctg tttcaaggct tgctgatata actaaattat ctaattcaaa atcctatgta
    36841 gtccacttaa gttgtaagga gtcacttaaa actgtaaaaa tggctagaga aaaaaatgta
    36901 gatatgatag tagaaacatg cccacaatac ctattattag aagacaattt atataataag
    36961 gataaatttg agggtgctaa gtatgtaatg tctcctcctt tgagaaaaaa agaagacata
    37021 aattgccttt gggaagggtt agcgagtgga gatattcaaa cagttggcac agaccattgt
    37081 tcatttaatt ttaagggaca aaaagacttg ggaatagatg attttagtaa gataccaaat
    37141 ggagcacctg gtgttgagca taggttagcc cttttatata catatggagt cttagagaat
    37201 agaataagtg caaataaatt tgtggaagtt acttctacga acgccgcaaa aatatttgga
    37261 atgtatccta aaaaaggcga gatagctgta ggaagtgatg cagatatagt cattttaaat
    37321 attaataaag aagaaacaat tagttataaa actcaaaaac aaaatgtaga ttatactcca
    37381 tatgaagggt ttaaaattaa atgtaaagtt gaagatgtat ttttgagagg aaatcatgta
    37441 gtgcaaagtt gtaatgttaa agaacagcca ataggacaat atattaaaag aaaaattact
    37501 aaataatgcg gggggaaata agaatgatgg atatacttat taagaatgcc ataatagtaa
    37561 ctgtaaataa agagagagaa gttatttttg atggggcatt ggtagtaaaa gataacaaga
    37621 ttgctgatat tggaaattct aaagagatag aaagtaaata tacggatgta aaaaaaatta
    37681 tagatgctaa aggtaaagtt ttattcccag gttttataaa tacacatact catttattcc
    37741 aaactttatt aaaaggattg ggcgatgata tggtacttaa agattggcta gaaactatga
    37801 catttccagc agctaactat ttagaaccaa aagatactta tgatgctgca atgcttggat
    37861 gtattgaagg tcttagaagt ggtataacaa caatggttga ctatatgtat cctcatagta
    37921 agccaggtct ttgtgatgga ataatagatg cttataaaga attaggaata agaggaatac
    37981 ttggaagagg atgtatgaat acaggagctc aatttggagt tcatcctgga ataatgcaag
    38041 atgtagaaac ggttgaaaaa gatgtacgaa gattgtttga aaaacaccat aatacagaaa
    38101 atggaagaat taaaatagga gtagctccgg cggcaatatg gtcaaattct caagaaatgc
    38161 ttgagatgct gtggagagta gtaaaagaat atgatgatgc tttatttaca gtacatatat
    38221 cagaaactcc ttttgataga gaagcagcaa aagaattgca tgggcaatat gacatagatg
    38281 ttttagaaaa attaggaata cttggaccaa atgtacttat ggtacattgt gtgtatctaa
    38341 ctgaaagaga tatggaactt gctaaaaaat atgacatgaa agtgtctcat aatacagcaa
    38401 gtaatatgta cttatcttca ggtgttgcac cagtgccaga aatgcttaaa aaaggaataa
    38461 cagttagctt aggtgtagat ggagctgcta gtaataactc tcaagatatg cttgaactta
    38521 tgaagttaac agcacttcaa cataaagtta ataagtgtga cccattagca atgtcagcag
    38581 aaaaagttct tgagttggca actatagatg gagcaagagc tattggaatg gaagatgaga
    38641 taggttcatt ggaaataggt aaaaaagcag acttactaat attcaatcca atgctttcac
    38701 caaaagctat acctatgcat aatccagtat ctacattagt atattcttct agcatgaaaa
    38761 atatagaaag tgttatagta gatggaaata taataatgga agattcgaaa atattgacaa
    38821 ctgatgaaga taaagcatta aaagaggctc aagatacagc tgagagactt tgtgtaagag
    38881 gaactatcaa gaatagaatg gaaggtcata aatggaatag cctttattaa taaatgtaat
    38941 ttattcataa aaaagtatag taaaaacttt aacttaaaaa tatgagtttt gaggtgattt
    39001 atttgattat caaatgtacc ataaataata aagaaaaaga attaagcata agaccagatg
    39061 attttttagc agatacatta agagaaaatg gatatgtaag tgttaaaaaa gggtgtgata
    39121 caggctcatg tggcttatgt acagtgatgg taaatgacaa ggcagtactt tcatgtaatt
    39181 atcttactgc tagagtagat ggtcagcata ttacaacact tgagggtgtc ttggatgaag
    39241 cgaaagaatt tggagatttt cttgcagatg aaggggctga ccaatgcgga ttttgtgctc
    39301 ctggatttat aatgatggtc ttggctatga aaagagagct tcaaaatcca acagaagaag
    39361 aagttataga ttatgtaaat ggtaaccttt gtagatgtac aggatataaa ggtcaattaa
    39421 gagcaataca taaatatttg aacacttaga cttttaagtg aaaattaagt tgatatgggg
    39481 agagagtatg ataactataa aggaatatgt agttccaaaa agtttagatg aagcttatga
    39541 actgctaata tcaagaaaaa ataacataat acttggagga tgtggatttt taaaactagg
    39601 tagtaaaaat attggttcag caatagattt aaaaaactta gctttagatt atataaatga
    39661 aactgatgat agtatattaa ttggagcaga tacaagttta agaactttag aattaaataa
    39721 ggttattaaa aattactgta atggggtaat ttcaaacgct gtttctaata tagtaggagt
    39781 tcaatttaga agtggagcta gagtaggagc tagcgtattt gctaagtatg gattttcaga
    39841 tttaatacct tcattactag tggtagatgc aaaggttaag ttatataaaa aaggggttat
    39901 gagcttagct gattttttag aaagtgagct tgaaaaagat atattaatag aagttatatt
    39961 acctaaaaaa gatgctatag gtgtatttga ttctataaga aaatgtacag gtgattttgc
    40021 agtattaaat ggagcaatgt taaaagaagg aagtatttat aaaattgcaa taggtgctag
    40081 accaagaaga gcaaaaattg cctataaagc aagtgaaata ttaagcttag aaaatgatat
    40141 agaaaaagct ggcgaagtat caagtgaaga actttcattt ggaagtaata taagaggaag
    40201 taaagaatat agaaaagata tggcaaaagc cttagtatta agaatgtata atagtattgg
    40261 aggtgagtgt gatggtaaat aaagaggtaa gaaaaataga tagtgaagca attgttactg
    40321 gaaagccaat atatacagaa gatttaattt ttcataaaga tgttttaaca attaagcttc
    40381 ttagaagtcc acatgcattt gcgaagataa aaaacataga tataaaaaat gcgttaaagg
    40441 taaaaggtgt agtaaacata ttcacttaca aagatgttcc aagttataga tattcaatgg
    40501 ttggggagtc atatccagaa gcatcacctc atgaccaatt aatacttgaa gacgtagtta
    40561 gatatgtagg agatgaagtt gctataatag ccgctgaaga tgaaagggct gctattaaag
    40621 ctatgaaact tataaaagtt gaatatgaag taatgactcc tatattggat gcaagagaat
    40681 cagaaggaaa taaaatttta atccatgaag aagatgattt gttttgtcca tttgattttg
    40741 gattggatgc tacaaaaaat atcgtatcaa gagaaaaaat gggtaaaggg gatgtagaaa
    40801 aagagtttaa agaaagtgat gtaatagtag aacattctta tatgactcaa tcacaagccc
    40861 attgtatgat ggaaactcat agagcttata catatataga tacgaatggc agattagtag
    40921 taacttctgc aaatcaatca gcttatcata tgagaagaca ggtttctaga gcattgggga
    40981 tacctctttc aaaggtaaga gttataaaac caagaatagg tggaggtttt ggtggaaaaa
    41041 acatagctgt aacagagata tatgcatcat ttgtaacttg gatgacgaaa agaccagcaa
    41101 aactaattta tactagagaa gaaacttttg caatgactaa tactagacat cagatgtttt
    41161 ttgatataaa aattggggca gataaagaag gaaacataaa agctatagat atgaaagcat
    41221 tgaacaatac aggtgcttat ggtgataatg gaccttcagt ttgctctgaa tcaggtcata
    41281 atgtacttcc aacatataat aatgtacctg caataaaatt tgatgggaaa actgtatata
    41341 ctaatttagt tccaggggga gcattaagag gatatggagc aacacaaggt acatttgcac
    41401 ttgattgtgc aattgatgaa ttagcgcata aacttaatat ggacccaaca gaaataaagt
    41461 taaagaatat tataaaaaaa caaactgaag gcggaataat gaattttcca ataagaagtt
    41521 gtgcacttga tgaatgtata attagaggta aaaaacttat tggatgggat gaaaaatacc
    41581 ctgttaaaga tattggaaac aataaggtga gggctgttgg aatgtctgtg ggaactcatg
    41641 gttctggtat agccaacata gatatggcaa tagtaaatat gaggatggat gaagatggga
    41701 cttataagtt gttttctggg tcttcagact tagggactgg ttctgacaca atacttgcac
    41761 aaatagctgc aaaagcattg aacacatcta ttgacagaat ttctgtttat acaggagata
    41821 cagatatgtg tccatatgat actggggcat atgcttcttg tactacttat gtaactggaa
    41881 atgctacaat aaaatctgct gaaagtttaa aacgaaagat attaaaagta gcacagaaga
    41941 aacttgattt accaatagat gatttaatac tatatgaaga tagagtttgt tatagtgagg
    42001 atgtagagaa gtttgttttg ttaagtgaat taggaaaaga atctgttggt ggtggaaatc
    42061 aagaagtttt atattcatct gaatcttatg gtatacaaga gagtgctaaa ccattcttag
    42121 caggatttgc tgaaatagag ttagataaaa caacagggga gtttaaagta ataaattatg
    42181 cttgttctac agattgtggt acagttatga atcctgcact tgctagaata caagttgaag
    42241 gtggtgtaac tcaaggtata gggcttgcaa tgtatgaaga ccctagacat ggttctgatg
    42301 gaaagcttca gacttctaat ttattacaat acaaagttcc aacaaaaaaa gatataggaa
    42361 atataattgt tgattttgta gataattatg agccaacagg tccatatgga gctaaatctt
    42421 taggagaaat agttattcat acacctgcac cagcaatagc aaatgcaata tacaatgcca
    42481 caggagttcg tataagagaa cttcctataa cttctgagaa agtttatatg gccatgaaaa
    42541 aattaaatca gggaggtaaa taaatgattg ctattaaaag taaatactta atatcttctg
    42601 ctgatgaaat atatacaaat tatgctgttg tagtagaaaa tgacattata aaagatatat
    42661 tgccaaatga agaagttgaa ataaaataca aaaatattga agaagtaata gataaatcag
    42721 atgctataat aatgccagga tttgtaaatg gccatatgca tcaatatgga gttctttcta
    42781 gaggaatacc ggcaaatgta cattttactg actttgaagg tttcttaaat gactattggt
    42841 ggccatttat agaaaataga attggattga aagaagtaaa ggcaacaact aaagcatcag
    42901 caatagaact tatagaatct ggtgtagtag ctttttgtga taccctagaa gctccaaata
    42961 cagaagaagg aactctaatt gaacaaggaa aaattctaga agagataggc atgaaagcgg
    43021 ttttatcatt agaaagttgt gagagaataa gctttgaaaa tgggcttaga tgtttagacg
    43081 aaaattctaa tttaataaag tggagtagag aaaatagtaa attaataaat ggaataatgt
    43141 gtacacatac aagctttaca tgttcggata aatttattaa gaaagctaaa gaagatgcaa
    43201 agaaattaaa tgcaccgatg caatttcatt tatgcgagag tatatatgaa ccaaactatg
    43261 ctgaaaaaca ttttggcaaa aaagcagttg actactataa tgatttagat atattggatg
    43321 aaactgtatt agcatctcag tgtgtaaaag taaatgatga agaaatagat atattaaaag
    43381 aaaaaggcgt aaaagtagtt catatgcctc ttagcaactg tgaagtaggt ggaggttttt
    43441 ctccagtacc aaaaatgatt aaaaaaggca taaaagtggc actgggtact gacggatata
    43501 taaatgattt ttttgaagtt atgagaggtg catttttaat gcataaatca gttgaagaag
    43561 atgcatcagt aatgccagca aatttagtat ttagaatggc aacagagcat ggtgctcatg
    43621 tattaggact tcaaaattct ggtaaaatag cagttggtaa taaggctgat ataatagtta
    43681 tggaagatga atttaaaact cctgtgactc ttgataatat atttgaccag atagtagttc
    43741 agggtaaaaa agaatttata tcaaatgtat acatagatgg tagacatatt ttaaaagaaa
    43801 aacagctagt tgatttagat aaaaaagcta ttgttaaaga aatgaaagaa gttgcttgtg
    43861 aattttggaa tttttaacga atgtaaatat atatgaaaag gggaagcaga taatgtacaa
    43921 ttttttaggt aaagaattaa aatcaccact tataataggt tcagggccat taacatatag
    43981 tgcggctgga tgcaagatat taagtgatgc aggagcaggg gcagttgtta ctaagactat
    44041 aagaaaagaa agagctataa atccagcacc acatatggtt agaaatacag ctaatgctct
    44101 tttaaataat gagaaatgga cagattttga gccagagcaa tggatagatt ttgaaatacc
    44161 acaaatgaaa agagatggga ctgtatgtat agcttcagta ggtcatacaa ttgaagaaag
    44221 cagcgaatta gtagaaaaag ttgcaaatgc aggagcagac tttattgaat tagtatctta
    44281 tgattataga gatttgatac caatgcttaa agatgcaaag gaaagagtaa atataccagt
    44341 aatagttaaa cttcctccta tgatagatga gataggcgat tttgccaaaa aattagaaga
    44401 agcaggagca gatgcaataa ctgcttgtga ttctgtagga ccagcattta gaatagacat
    44461 agaaacaggg caacctttat tagggggaaa tggaatagga tatttaagtg gagaaactat
    44521 caagccaata actttacaga gaatatatga aataaaaaaa caagttaata taccaataat
    44581 tggtctaggt ggatgtgtaa gtggagatga tgccttagaa atgattatgg caggtgctga
    44641 ctttgttggt atatgttcag tagtaatctt aaaaggtgca caagtaatat ctaaaataca
    44701 tgatgactta aaatctaatc ttaatagatt aggatataat actatagaaa atgcttgtgg
    44761 aatagtacat aatcatatgg gagatgttaa tgaaagactt agctttgagt ttacttatga
    44821 tgaagaaaaa tgtactaaat gtaagatgtg tgagagagtc tgtgcatatc aagcgagaaa
    44881 attgaatgat aaaatggaat tgaatgaaga agagtgtaga tattgtggac tttgtatatc
    44941 agtttgtaaa ccaaaagcac ttggaagaaa aatttcatgt tcagagttga atgtatagat
    45001 tattttataa aaaataaagt tttaaataaa aataagaagg cctatctcaa aatagagata
    45061 agcctttttt gttgacctta tttttgttta tttatagtta ttttagatat taattagttt
    45121 tgaagtaata aagtgcgtaa attttgttta attaaaattt taataattct tatatatatg
    45181 ttaacattgt atagcatttg tgtaaaatgt tataataatt aatagggatg gtgatgcaag
    45241 tgcaagagaa gattttagaa ttattgtata ctataaatga agatgtagtt agcataaaaa
    45301 atgatatgtc agaagtaaaa caggaaataa atgtgttaaa agaagatatg tcagaagtaa
    45361 gacaagaaat gactgtaatg aaagaagata tgtcagaagt aaagcaagaa ataaatataa
    45421 tgaaagaaga tgtgtcagaa gtaagacaag aaataaatat attaaaagaa gatatgttag
    45481 aagtaaaaca agagataaat ataatgaaag aagatatgtc agaagtaaaa caagagataa
    45541 atataatgaa agaagatatg tcagaagtaa gacaagaaat gactgtaatg aaagaagata
    45601 tatcagaggt aaaacaagag ataaatgtat taaaagaaga tatgtcagag gtaagacaag
    45661 aaatgactgc aatgaaagaa gatatgtcag aggtaaaaca agagataaat gtattaaaag
    45721 aagatatgtc agaggtaaga caagaaatgg ctgtaatgaa agaagatatg tcagaggtaa
    45781 aacaagagat aaatgtatta aaagaagata tgtcagaagt aagacaagaa atgactgtaa
    45841 tgaaagaaga tatgtcagag gtaaaactag aagtaactgt aatgaaagaa gatgtttccg
    45901 aagttaaatc agaagtaaac tttatgcaaa ataaaataaa taatattaat aaagatatga
    45961 gtggaattaa ggaagaagta tccatagcaa atgaaaaatt agatggaatt gaaattaaga
    46021 ttgatagtct agaaagtgaa gataagtcaa tgaaagagat gcaggtagaa cagaataata
    46081 ttttaggaag cttattacat aattctgaaa taaataaagc tactcatgat aatatagaac
    46141 ataacatagc atatataaaa ggagatacta attctataaa agaggatata gctgaaataa
    46201 gaagagactt aaatttagta gagttagcca ctagtaaaaa ttggagtgat attgtcaaac
    46261 taaaatcagt aaagtagtat tttaattcac taatttatca tccacaaaca taaaagaatt
    46321 tatagtgttc ttactattta ttataaattt tagaaatgaa aataaaaaat tgagaaaaga
    46381 ccgttaaaaa tttattttta ggtctttttt ttatgcaaat ataactgatt tataaaaata
    46441 aaacctaaaa tttaaatgat aaaatctaat agaacgattg aaataagtaa aattttatca
    46501 ataaaaacat aaaaatttta tactatgtga atgaatgcaa atattggcac taaaattgct
    46561 ctataaaaat tataaatagt aatatgaata aaattttttc tataagaaca atattactat
    46621 tttacttata gatatatata tatctataaa tagatagaaa cttgtatatt ttatgttgca
    46681 gatacaaata tagtaaagga gagtacattg gtacaactga gatacatagc ttattaagtt
    46741 tttctgtctt atgagaatta tattataaaa tattcataaa tattttgagt ttaaattaaa
    46801 aaattatttt atgcttagat taattgctat ttaattaatt taataacaaa aacagtgaaa
    46861 gagtaaatag tattatttac agggggtaaa gcaaagtata ttaaataaat tgtactttta
    46921 gccccgtaca tagagaaatc tatgtaggac acaactttaa aaccagtaaa tcctaaagcc
    46981 ttacaactac aataaaagat agaaagaaac ttttatgaat gtggtgaaaa ccagaaataa
    47041 tagtgaggat ggtatatggt gagagcctaa gtacttaaaa atggacattt ggtcgccaag
    47101 cctagaaata ggaaggtcaa acgactatct cgagagagag tacactataa gtgattgata
    47161 gtggaaacgg gttgctctta gcaggtaatg ctgaagatga agaaatagtc tacgctcata
    47221 tgaaagtatg aggtcactgg atttgaagtg acatctataa agtagcgttt ataggtaaat
    47281 gtttaaaact tgaaaaatat aaatacttat gaaaacttat aacttataat gattaagatt
    47341 gaaagattta aattgaatta aatttactat aaaaaattaa aaattcatag ctataatact
    47401 cttatatttg taaagcatta tatataatat atgcaaaatt aaagaataag tttatttgtt
    47461 aataattaaa tttattgtat ccttttttag accaaaaact taaatatgtg ttggagtata
    47521 gttgcgattt atgaacatca ttaatttaat atattagatt tttaagtatt tatataattc
    47581 aataagtttt atgtaacggg agtttttatt atgaaagcaa aaagtacatc aaaatttgaa
    47641 ttagacggaa tgccaccact aaaggaagca atacctttag ggttacaaca cttaatggca
    47701 atggtaatag gtagttgttt accagcgata ttgattgcaa atgcagctgg acttaatcat
    47761 aagatgtcaa cattattgat tcaagccgca ttaatttttg caggattatc aactttatta
    47821 caattatatc caataccttt gtttaaaggt tttaaattag gaggaggaat accagttata
    47881 atgggagcta cagctatgtt tataggagct ggggtatctg ttgcaggtaa gtatggacta
    47941 ccagttttat ttggttctca aataatagct ggtatagtaa taattataat tggttttggt
    48001 cttaaaaaaa tacgatttat atttactcca gctgtatctg gaacaatagt tgcatgtatg
    48061 ggtcttgggc tttttccaat agctataaaa aacttagctg gaggtatggg aaatgaaact
    48121 tttgggaatc aaattaattt tatagttgga atatctgtat ctcttatgat attaggatta
    48181 aacaagtatg gaaaaggatt atttaaagat gcatcaatat tagttagcat aatctttgga
    48241 tacattttat cattaatttt aggaatagta aatttttctt caattcaaga gtttactttg
    48301 gttgcacttc caaaaccgct tatttttggt ctagatataa gacttgaagt tgttgtaatg
    48361 ttttcaataa tatatttagt tgagatagct gatataatgg gtgcctgcac actttctgcg
    48421 gtaggagggc ttaatagaca agtaacagat gaagaacttt cttctgctgt tttaggaaat
    48481 ggagtactct ctacaatagg tgcattattt tctgcaactc caatggggat gtttggacaa
    48541 aattctgcaa tcgtttccaa tacaaagatt gtaagtaaat tcgtactagc tattggagga
    48601 attggactat ttttggcggg aatatcgcct ttattagcta atttaataag aacaattcct
    48661 ccttgtgtgg ttggaggggc aacacttgtt atattttcaa ctcttacaac atctggttta
    48721 cgacttgtta gtatggatgg atttaatcaa gaaaatagca tgatattagg tctttctatg
    48781 gcttctggta ttgggttcat ggtagctcct caagttcttg aaagatttcc aaagtttgta
    48841 gaaacattac ttgcagattc aagtgtagtt tcaggagcta tggtagccat aataatccaa
    48901 gctttatata tggtaaaatt tcctaaaaat aaaaatggta aagttgagga taaaaataaa
    48961 agtttataat gtaaagattt tatacatgtg aattaaatat actacaatta taaatataaa
    49021 taaaaagatt tattaaaact cttatgtttt aataagctaa tgatgggggg ataagatggt
    49081 tactataaag gaatattcag ttccacagag tttagaagaa gcatataaaa ttttaacttc
    49141 taaaaaaaat aatgttattt taggtggatg tggattcata aaattaagca ataaaaatat
    49201 aggaacagcg atagatttaa aagatataaa tctaaattat ataaaagagg atgaaaaaaa
    49261 tattttgata ggtgcagata cttctcttag aagtttagaa atggataaaa ctataaaaaa
    49321 ttattgtagt ggaatacttt caagtgctgt ttctaacata gttggtgttc aatttagaca
    49381 aggagctaga attggtgcaa gtgtattttc aaagtatggt ttttctgatt taataccagc
    49441 acttttggtt gtcggggcaa aggtaagatt atacaataaa ggaatgctgg agttgaatga
    49501 gtttttggat agtgaattaa gtagggatat attggtcgaa gtaatactac cgaaaaaaga
    49561 tgcgatagct gtttttgact caataagaaa gtgcacagga gatttttcta tattaaattc
    49621 agcaatgtta aaagaaaata atacttacaa aatagcaatt ggggcaagac ctcaaaaagc
    49681 tagattagca cttgaagcaa gtaatatatt aaataaagaa aaagatatag ataaggcatc
    49741 tattgttgct agtaaggaac ttacttatgg aagtaatatg agagctagca aagagtacag
    49801 aaaggatatg gcaagtgcat tagttaaaag gatgtataat gctattgagg agggaatgta
    49861 taatgattaa taaggggata agaaagatag atagtacagc aattgtcaca ggaaaacctc
    49921 tatatactga agacctcatt atgcataaag atgttttaac attaaagtta cttagaagtc
    49981 cacatgccca tgctaagata aagaatatag atacatcaaa agctgaaaaa attccaggag
    50041 ttgtgggtat ttatacgtat aaagatgtac cacaggtcag atactgtgtt ccaggccaaa
    50101 cagagccaga agcatcacct tatgatagag ttttacttga agatagagtt agatttgttg
    50161 gagatgaagt agctttagtg gccgctgaag atgagaaaat agcactcaaa gccttaaaaa
    50221 tgataaaggt agactatgag gttttaaaac ctgtgtttga tgcaagagaa gcagaaggaa
    50281 atgaagttgt tatacatcca gaaaaagaca tagtagagcc ataccctttt ggattggatg
    50341 caaacaataa tatagtatca aaagaatttt ttgaatttgg agatgttgag gctaagctta
    50401 aagaaagtga tcttgtggtt gaatcttcat ataaaactca aagtcaatca cattgtatga
    50461 tggaaactca taggtcatat tcatataaag atataaatga tagaattgtt gtagtttcat
    50521 cattacaatc accacacaat gtaaagagaa tccttagaaa agtattgggt ataccatctt
    50581 caaaaataag agtaattaaa cctagaatag gtggtggatt tggaggaaag aatatagcag
    50641 ctacagatat atttactgcg tttgttacca tgaaaactgg tagaccagca aaatttgtat
    50701 ttacaagaag tgaaactttt gtaggttcta atagtagaca tgaaatgttt tttgatgtta
    50761 aagtcggtgc agataaagaa ggaaatttaa aggcaataga attaaaatca ttaaacaata
    50821 caggtgctta tggtgggaat ggtgtctctg tttcttctga atctggacac aataccttac
    50881 caatatataa tgatattgat gctgttagat tcaatgctag aacagtatat actaataggt
    50941 taccagcagg agcacttaga ggatatggtg ctacacaggg aatatttgca ttagatagtg
    51001 ctattgacga attggcacat aagattggaa tggacccact tgaatttaga attaaaaata
    51061 taatagatag gtatactagt ggaaaagcag ctgaaggtga aatattaagt tgtgagttga
    51121 aacaatgtat agaaaagggg aagaaattaa ttaattggga tgataaatat ccatgtaaag
    51181 atataggaaa taacaaagta agagcagtag gtatggcagt agcaaatcat ggttctggta
    51241 taccacgtgt agatatggca acagtgacta taagaatgga tgaagatggc acatacaaat
    51301 tattatctgg tcttgctgac ttaggtcaag gagctgatac aattcaagca caaattgctg
    51361 ctgaagcttt aaatactact gtagatagaa tttctattta tacaggtgat tcagatttat
    51421 gtccatatga tacaggagca ttcgcttctt gtacgacaac ggttacagga aatgcaacaa
    51481 taaagacagc aaataaatta aaagatatat tactttcagt tgcggagtca aagctaggat
    51541 ttaaaaaaga agatttagag ttgggaaaag acagggtttt tgttaaaaac gataatagta
    51601 agtttgtttt attggaggaa ttgggtagag aatctgttgg aggaattggg tttgctagtg
    51661 atgatgtacc agtagttttg gaagcaacag aatcttttgg tatattaaaa aaaataaaac
    51721 catttatagc aggatttgca gagatagaat tagataaaaa tacaggcgaa tataaggtaa
    51781 taaattttgc atgtgtacct gattgtggaa cagttataaa tccaactttg gctagaatac
    51841 aagtagaagg cggtgtgatg atgggtatag ggtttgcaat gtatgaagac cctagatttg
    51901 ataaagatgg gagattatta acaaatagtt tcctacagta taaagttcct acaaaaaaag
    51961 aagttggaaa tatattggta gattttgctg agaatttcga ctcaggaggt ccttacggtg
    52021 ctaaatcatt aggagaaata gttatacata caccagcacc agctatagca aatgcaatat
    52081 ttaatgctac aggagttcgt ataagagagc ttcctatgac ttttgaaaaa gtttatatgg
    52141 gtatgaaaaa taataataaa caattataat aagtaatatg ttgtataaaa acttgtaaat
    52201 ttaaattatt ttgtaaaata aagtaattaa atggacatta tatatggtgt tttataaaaa
    52261 taccgtatat aatgtctagt agagtatatt ctaaaggagg tgagttaatg tcaaacagaa
    52321 taaaaaatat acactatgga acccagagac aacaattaaa aaacttaact aaaaaagaat
    52381 atcaagctct acgagagatg tgcttcttag ccaagaactt atataatgtt ggtacatata
    52441 atgtgagaca acattatttt aatcaaggag aacatcttaa ctatgaagct aattaccatg
    52501 tatgtaaaga aaatgagaat tataagcttc tcaatagtaa tatagcgcaa caaatattaa
    52561 aagaagtcga cagttcattt aggtcgtttt ttggattaat taaattagcc aaagaaggta
    52621 aatatgattt tagagggata aagtcaccta aatatttaga taaagaagga ttttttagta
    52681 taataatagg tcaaataaga atcaaagaag atgggactct aaatgtacct atgtctccag
    52741 tatttaaaaa actatatgga aaagtaagta taaaagttcc tcaaaatata ctagagaaaa
    52801 aaattaaaga aataagaata atacctaaac ataaagctag gttctttgaa attcagtatt
    52861 gttatgaaat accgaaatca gaggaagttt caaatcaaaa aaatgcacta gctatagatt
    52921 taggtttaga aaatttatgt acatgtgtta ccaatttagg tgatagtttt atcatagatg
    52981 gcaaaaggct taaatctgtg aatcagtggg caaacaaaca gaatgataaa atagaggcct
    53041 taaaattaaa aaagaatata actgcaataa caagaaaaca atttaaagtt tggaataaga
    53101 gaaataatca agtcaaagac tatgtaaata aaacttgttt ttacataatt gattactgta
    53161 taaaaaataa tattggtaat ttaattgtag gatataatgc taagttagaa aaaagtgata
    53221 atatggagag aaaaatatat agaaatttta taagaatccc ttttggagag ataaaaggaa
    53281 aattagaata tctatgtaaa ataaaaaata tcaattttat taggcaagaa gaaagctata
    53341 ctagtaaatc tgattttttt gccaatgatt ttctacctga tatcaatgtg gacaatgtga
    53401 aaaaatatga ctttaaaggg gaaagaataa cacgtggaca gtataaatcc agtgttggaa
    53461 tcattataaa tgcagatgta aatgcgagtc ttaatatatt aaaaaaatca caagtagtta
    53521 atttatctaa tttgcagaat aatcctcaaa tattaaagca accacagaga gtaagaatat
    53581 cataattata tttaaaagat aaactgatag cgtaagctta cagcttagcg gcgaattgga
    53641 tccgccttgt tgtttacaac agaatctatt tactttcgag tttatggaat ttcaaaaatc
    53701 acttttacta aaaaggtcta gctgtaaaaa ttaatagatt agaaaactgg ttataagagg
    53761 tgaaagtgtg aaactatcaa atataataga aaagaatgaa ataataactg tagttggagc
    53821 aggaggaaaa acttcattta taaactattt tgcaaatttt tatagagata aattgaaagt
    53881 tctacttaca acaacaacta aaatatatgt accaaatgat tatgacaata taattactac
    53941 aatagatgga actattattc catctatttg tcatggaata atagtatgtg gtagtcatat
    54001 aaataatgaa aataaattag taagtattga ttcatctatt ttggatgaaa ttgtagacaa
    54061 atttgattta gttttaatag aaggagatgg ttcaaagaga aaaaaactta aaggttggaa
    54121 tactaaagaa ccagtagtat atcataaaac tacaaaaact ataggtatat tagatataac
    54181 atcatttgga atggatatta atgaagaaaa tatacacaga gtagaaatgt tcaaaaaaat
    54241 agcaaattta aatacaaaga gtattaatag tagctctaca gtaagtattg aaaacttaaa
    54301 aaatatagta ttaaatccaa atggattatt taaaaattat tctggaaaaa gagtattatt
    54361 tattaataaa gttgaaaatg aaaaatataa aaatttagct ataaaattaa taaaaaatat
    54421 taaagaatgt gaaagtgaaa tagagatatt ttatggaagt gtaaaacaaa aattttgtgc
    54481 aagatattga agtattaaaa tagtttatat gatattatat atatagataa tatcaataga
    54541 gatattataa aaatcataga actatcaata tattataaat atactataaa taccaaggat
    54601 gatatatatg ataaatgcag tgataatggc atccggtttt gccagaagaa tgggagtaaa
    54661 taaactttta ttagattata aaggatgttc tataatagaa catgtattta gagtagtcaa
    54721 taagattgat tttcatcaag ttgtagtagt aagtcaatat gaagaagttt tattattgtg
    54781 taataaatat ggttttgaat atatagataa taaaaatgca aatgtaggtc aaagtgaatc
    54841 tataaaattg ggagttttaa atagctcaaa atgtgatggt tatatgttct ttgtaggaga
    54901 ccagccattt attgataact tatatataga taaaattata aatactttta aattagatag
    54961 agattttata gtaattccaa gatacaaaga tatatgtgga aatccagtta tattcccata
    55021 tggtaagaaa gaagaattgt tagcacttaa agaagatgaa aagggtaaaa ctattttaaa
    55081 aaattcatca aaaataaagt atgtagaagt tcctaaagaa atgttatttg atatcgatac
    55141 aaaaatagac tatgaaagaa ttgggggtaa attatgaaac taataaaaac tatagatgct
    55201 gttggacaag ttttatgtca cgacataact caaataatac ctggcgaatt taaaggaaga
    55261 aaatttaaaa agggtcatat agtaaaagaa gaagatattc cagtgttatt atctcttggt
    55321 aaagataatt tgtatgtatg ggaaaagtca gaaggaatgg ttcatgaaaa tgaaggtgca
    55381 ttgtttttaa aagaactaac tgctggagaa aatttagatt ttagtgaaat aaaagaagga
    55441 aaaatagatt ttatagcagc atgtgatggt cttttaaaga tagatgttga tgcacttttt
    55501 gatttgaatt gtgttggaga aataatgatg gctactcttc ataataattt tatagtaaat
    55561 aaaggtttaa aagtggctgg aacaagagtg atacctttgg ttatagatga gaaaaaatta
    55621 gaagatgcaa aaaaagtagt tggtaataga aaaatagtaa atgtagtgcc ttttaaacct
    55681 aagaaagtag gaattgttac tacaggaaat gaagtatttt atagcagaat tgttgataaa
    55741 tttggaccag taatagaaga aaaagtaaag ggttttggtt gtgaagttat aggacagact
    55801 atatgcccag atgataaaga cgttataaag aatgcaataa aagaatttat aagtcaagga
    55861 gcagaactta tatgctgtac tggtgggatg agtgtagacc cagatgatgt tacaccaaca
    55921 gctataaaag aaactggagc tgatttagtg acatatggtt caccaatatt accaggagct
    55981 atgtttttac ttgcttatta tggcgaagtt ccaattatgg gtataccagg atgtgcaatg
    56041 tatcacaaaa ctacagtatt tgatatagtt ttatctagag ttttaattga tgaaaaactt
    56101 gataagtatg atatagctag atatggccat ggtggtcttt gtatgaactg tgatgtgtgt
    56161 acttatcctg catgtaattt tgctaaaata taaagctatt aaaaaagagt atttacaaat
    56221 taagtactct ttttaaatat gaaacaaaac aactagaatg atgttttaag aaaagcatat
    56281 ttatttaaaa gttataataa ctgattttca atgtgtaaaa gaatgaataa agttgcaatt
    56341 tgatttcttt ataagtataa tataagagat tgttaatttt cataaattta caatttttag
    56401 tattgtttta ataaatattt cacaaatttg gtataaaaaa tatactattt acgtgataat
    56461 agataataaa aatgttatca aagttttatg ataaaataaa ttatgagtaa aaagaaagga
    56521 aaatatatta atgttacaag ttacaggtgt tggacttaga tttggtgata aggaactatt
    56581 taaagatgtc aacttaaaat tcactaaagg taattgctat ggaataatag gagctaatgg
    56641 tgcgggaaaa tctacttttt taaaaatatt atcaggagaa atagagccaa atacaggtag
    56701 tatatctata actgataaag aaagaatgtc agttttaaaa caggaccact ttgaatatga
    56761 agaagaaact gttttaaatg ttgttataag aggtcatgag agactttgga atataatgaa
    56821 agaaaaagat gcattgtata tgaaggaaga ctttaatgaa gaagatggta taaaagctgc
    56881 tgaactcgaa ggtgagtttg cagaacttga cggatgggat gcagaaacta atgctgagaa
    56941 aatacttatg ggtcttggca taactaaaga tatgcattac aaacaaatga aagagttagt
    57001 tggtggggaa aaggttaagg tattgttagc acagtcgctt tttggtaagc ctgaaatact
    57061 attaatggac gaacctacaa accacttgga ttttaaatca ataaactggt taaataattt
    57121 tataatggat ttagaagagt ctatagttat aatagtatca catgatagac atttcctaaa
    57181 tcaaatatgt actaatatag ttgatgtaga ctttggtaaa atacaaatgt atgttggaaa
    57241 ctatgatttc tggtatgagt caagtcaatt agctttacaa cttgctaagg accaaaataa
    57301 aaagactgaa gaaaagatag ctcaattaaa ggaatttata gctagattta gttctaacgc
    57361 ttcaaaggct aagcaagcta catcaagaaa gaaacagtta gagaaattag aagttgaaga
    57421 tatacaacca tctagaagaa gatatccata tgtaggattt acacctgcaa gagaaattgg
    57481 gaatgaagtt ttagaagtac acaatttaac taaaactata gatggggtta aggttcttga
    57541 taatgtgtca tttagattgg atagagatga taaagtagta tttatgggtg atgaaatagc
    57601 tactactgca ctttttaaca tagttatggg agaacttgaa ccagacagtg gagaatacaa
    57661 atggggagta actacttctc aagactatct tccaaagaat cataataagt tttttgacgg
    57721 agtagaatat tctttggttg attggcttag acagttctct gaagaaaaaa gtgaaagctt
    57781 tataagaggg tttttaggta gaatgttatt ctctggtgaa gaagcattaa aagaagctca
    57841 agttatatct ggaggagaaa aggttagatg tatgcttagt aagctaatgc tttcaaatgc
    57901 aaatgtactt gtattagatg accctactaa ccatttagac ctagaaagta taacttctgt
    57961 aaataaagga cttgaaaagt tcccaggagt acttttattc acttctcatg accatgagtt
    58021 tatatctact atagcaaata gaattataga gataactcca aatggaataa tggatagaaa
    58081 aatggatttt gatgagtatt tagaaaataa agagatacaa gaacaattag ctaaaatgta
    58141 tggaaaagat aaataattat tagtataaaa ccttttaata tttgagacat gctatgcata
    58201 agtatgtaag tttttaatca aatattagga ggttttttta tgttatttaa atatgtttat
    58261 tagtagaata atattttgta gactaaaaat ttaaatataa tatagtagat gctataataa
    58321 aaatatagaa tattttaggg ggagtagatt atggttaaga aaaggttagt agctatagta
    58381 atgagtttag ctatgattag tgtaggaaat ttttcatatg ctcactcagg agggcataat
    58441 tcatcaggga gtcatcataa taaaaataaa tcagagttaa catattatta ttattgtgga
    58501 ggacattcag cacatcaaca caataaaggt ttttgtcctt atgctagtgc aaaatcaaat
    58561 tctggagtaa caatatcttc agcacataac gaaaaaaatc ataaaaaatt tttttcagaa
    58621 aaagagtata ataaaggtta tgaagatgga tgtaaagggc tttataattt aagtttcaca
    58681 gaagctaaat ctgaagattc atatgaaaaa ggatatttaa ttggatacaa tgaatataaa
    58741 gcaaaaaaag tatatgatgt aattaagaag atgttagata ttccaatgtt agcaagtata
    58801 taataaaata gtattttaaa agatattttc tataaggaaa tatctttttt attgtaaagt
    58861 acaatcttta ataaaaaagt ataataactt aggtgataaa ttaaaataaa acagattatt
    58921 taagcagtta ttaatttgag atagatacat ttaatatgaa tgaatctatc tcaataagct
    58981 aagctaagtt gatttaatat taattagcat taaataaact ttcagaaatt ttgtcagaag
    59041 cttctttatc catttcttta agtacatgag aataaacgct tagagtagta cttatatttg
    59101 agtgtcctac tctctcggat ataaccttta taggtacttt agaatttata agtaaagtaa
    59161 catgtgagtg tcttaaatca tggaatctaa tatgaggtaa ttcattcttt tctaaaaatc
    59221 ttttaaattt tttacttata acatcttcag caataggttc acatttctta ttaaaaaaca
    59281 acaaattatt attatttcta atggaagatt ttaataaatt tttattctgt tttattttat
    59341 attcttttaa taaagacatt aattctttgg gagaagatat tttcctgata gagctttctg
    59401 tctttggttc tttaagaata acagaaccat taattcggga cgttatctta tttactgtta
    59461 ttgtattttc ttcaaaatca atattatccc aagttagacc taaaacttct gaaattctaa
    59521 gacctaatcc aatagccaaa ttaataggta attccatttc tgtgtttttt gctaaatcta
    59581 ataattttat catttgttcc ctatcataaa tctcattttt aaacttttta tatcttggag
    59641 cctctatacc atccataaca ttttctttta ttaatttgag tctataggct ctttttattg
    59701 caagtttcaa tatatttata tgaatcttaa ttgattgagg atttagttta taagagagtt
    59761 tatctatata gttttgtatg tgaataggat gtaagtcttg tattttatat tttccaatag
    59821 atggattaat gtaattctta caaatactaa cgtaattgtt gtatgtagct aaagatatat
    59881 tttcgctata tttatttaga aaatctaata gaaattctga cactgtaatc tcgtttggca
    59941 ctaaaaaacc gtccttatat aagctttctt ttacctcatt tagtcttttg cttgcttccc
    60001 tttttttatc aaaaataccc atattttttt gttttctttt acctgtctct ttatcttcaa
    60061 attccaggta aacaacatag tttttatttc tttttctaat aaatgcattc atatataacc
    60121 caccaatcaa aattttatac atttattata acatatatat atcccaaaat gattttgaaa
    60181 tagagttgca tacattttaa tacattgtat atat
//
LOCUS       DAMJVM010000013        57403 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.013, whole genome shotgun sequence.
ACCESSION   DAMJVM010000013 DAMJVM010000000
VERSION     DAMJVM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 57403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57403
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.013"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            131..319
                     /locus_tag="PC523_000875"
     CDS             131..319
                     /locus_tag="PC523_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDF2474430.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            complement(479..631)
                     /locus_tag="PC523_000876"
     CDS             complement(479..631)
                     /locus_tag="PC523_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HDF2474431.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(729..1205)
                     /locus_tag="PC523_000877"
     CDS             complement(729..1205)
                     /locus_tag="PC523_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2474432.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDVIYKVFSKNGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1424..2185
                     /locus_tag="PC523_000878"
     CDS             1424..2185
                     /locus_tag="PC523_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2474433.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKKHHNKLLVKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIRENYGKDIIVASNKKFKNMSK
                     QDYEDWQNLSIEIISKLKKAFKTGDASSELAQEVARLHHKWLSYTWSTYSKEAHASLA
                     QMYVCDERFTSYYDKEQPGLTKFLRDAIVVYTRDN"
     gene            complement(2444..4372)
                     /locus_tag="PC523_000879"
     CDS             complement(2444..4372)
                     /locus_tag="PC523_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2474434.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSINE
                     TLEKNGAIIKIKKGEGYYIDILNLALYQHYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQKIVLSEINKFNINFLDFNLK
                     NFIIHLSITISRILGGYCLDNVLDVVLTDFQSNTAVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRADTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEISLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQNNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSRIYSNLLDNFFDKNLF
                     IVNPKIKEKDELIKLMCNKLTQSEIVIPSFTESVFYRESLSSTNIDDFLAIPHPMELS
                     SIKTKICISILNEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDSFLSIIKSII"
     gene            4607..5059
                     /locus_tag="PC523_000880"
     CDS             4607..5059
                     /locus_tag="PC523_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2474435.1"
                     /translation="MVLQEKNIFIDKDLKSKEEVLNFIADSAYILGITTNKDALLKDL
                     LKRESEFPTGIRDGFAIPHTKSANVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HENEGNIHLKTLSKLATCLMEDDFISEVKNASDASHLVKYITNKMKEE"
     gene            5062..5370
                     /locus_tag="PC523_000881"
     CDS             5062..5370
                     /locus_tag="PC523_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2474436.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHKIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGVALKKPEAVLTKCIETIS
                     "
     gene            5485..6591
                     /locus_tag="PC523_000882"
     CDS             5485..6591
                     /locus_tag="PC523_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2474437.1"
                     /translation="MSKKNNSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAM
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPSMGAGFIGGL
                     IASNTNAGFLGAVTAGFFAGYICKFLKKKIKISGSAAGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGSMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSRFSKTLSETGKGNIVMGIIGITEGAI
                     PFTLKNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISVGSGWAYLVGAIF
                     GALVIAILSTILVDFNEDETAENTEFDEIELDIM"
     gene            6665..9343
                     /gene="mngB"
                     /locus_tag="PC523_000883"
     CDS             6665..9343
                     /gene="mngB"
                     /locus_tag="PC523_000883"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDF2474438.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRKKLAIGPWYTQTDQLVISGESIVR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDTIGKLKVKSSTNNVYFPNG
                     FDQAPIRKNLPDILKKANKIDTENEYEISDIENYVDSVRNERNEFTELKGEFVNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLILKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDFHMRLISTQIKNNNEEITLTVFNGL
                     PEARNEVVEFDTYIPGQDFVIKNKNGEILNYIIKEKEDITDYILTQTIKLNPSKKIYI
                     PKTVYRAKIALSVKNIPPMGYTQLIVELDTKNKCEIKTSSCKKIENKYYKITANKNGS
                     VEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNVQEE
                     MTLKYSLTIPASLEERTIGKVSVKMPVTMKITLEPKEQIIKFNINIKNDQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTALEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQKGVALITGCVREYQIVGANLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFNFGLYIYNNKFDKSNIANTAKRFLTPIQIYEYADFLNGRLIFVFNDEKQ
                     IYENEYSFMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDSKGSLNINKNINAAYN
                     AMLDEIYDNTNKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(9622..11955)
                     /locus_tag="PC523_000884"
     CDS             complement(9622..11955)
                     /locus_tag="PC523_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2474439.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQAGAKGGA
                     KNPFEQESFVDNMYKGSYVLYHNMKEEQEGKMIQPSKLFISKETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNQNFENWESFIKESGKNLKYYLVDKESNFEFFNDNKELGALD
                     TTKNYASKEDSDENKSKNTEMSDEERQKQIKDIKENYRFYIVMNFDKDGKVNVLDSYG
                     VNQKVISQMLLSKAREDLMNTTLGGNPISYKLSPIKNVTLVYAVPKVISKGDGGEYSY
                     GNIYNNDSISSYINNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIILAILQGIVDLYPTSIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIKEYFIKNTVTGMFFVWLIKSGKVVINQIRITDLK
                     EKPNKTIAIILGINLLIIVIMCSFWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLEVNMERDLGFFNPIKDELGNIQAGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKVNSGD
                     VHLNIMNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVSIMLKNMSATEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(11924..12646)
                     /locus_tag="PC523_000885"
     CDS             complement(11924..12646)
                     /locus_tag="PC523_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012422616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2474440.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIIDIMMPKMDGITLTMKLRENHNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVENKEQKVDDDDGVFAIGGLELNENTKEVSVDG
                     KHIKATPIEFKILSLLMRNAGRVFSADEIYERVWNDNAVNTDTVMVHVRNIREKIEVD
                     PKNPKYLKVVWGVGYKIEKIQR"
     gene            complement(13041..13697)
                     /locus_tag="PC523_000886"
     CDS             complement(13041..13697)
                     /locus_tag="PC523_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2474441.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVIIKRRDLMEKIADVITKKQQEILVEMEKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            complement(13752..14381)
                     /locus_tag="PC523_000887"
     CDS             complement(13752..14381)
                     /locus_tag="PC523_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2474442.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSKHKEIDLSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERNNLLNECSLKECEYLSENGAKLFDDIENTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFVDFECPGNTGLHKGENNKLIIERNKLKNPI
                     YIGDTQGDANSAKFAGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            complement(14512..15153)
                     /locus_tag="PC523_000888"
     CDS             complement(14512..15153)
                     /locus_tag="PC523_000888"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDF2474443.1"
                     /translation="MKICIVLNGEIEDYEVTRGIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     KGDAKGVTLTGLEYPLDNYYMRYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            complement(15162..15833)
                     /locus_tag="PC523_000889"
     CDS             complement(15162..15833)
                     /locus_tag="PC523_000889"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF2474444.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPIDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVDKVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            complement(15838..16722)
                     /gene="rsgA"
                     /locus_tag="PC523_000890"
     CDS             complement(15838..16722)
                     /gene="rsgA"
                     /locus_tag="PC523_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF2474445.1"
                     /translation="MLEGKIIKGISGFYYVDTHNGIYECKARGIFRKHKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKKIYELSGYKVIPVSNITKLNIEKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVANGEISKERY
                     ESYIQLLNEIRQNNSRRY"
     gene            complement(16820..17557)
                     /locus_tag="PC523_000891"
     CDS             complement(16820..17557)
                     /locus_tag="PC523_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2474446.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTRLNEENFSIKLFKADVSNREDVENMVDYCIKEFGGLDVLVNNAGISQDKLFTDITD
                     VDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDILVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            complement(17665..19668)
                     /gene="pknB"
                     /locus_tag="PC523_000892"
     CDS             complement(17665..19668)
                     /gene="pknB"
                     /locus_tag="PC523_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDF2474447.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPILAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     EEEEEIMRAKKNQRTKSAPSKRTKKKKKKQESPKSRRRLKVIATVLILILCAQVFLAY
                     KFLFSGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCIISQTP
                     EGGSTNIKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYEYSSI
                     YKEGTVLNQSPGAGSSRVQEGDEISLYVSKGSEKSNTQTPTVPNKTPTTPNNDTPTEP
                     GSNSGSSGGSSGGSNSGNSGGNSGNNGNNSGGSNSGNSGGNSGGSNSGDNGGNSGGSN
                     SGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            complement(19685..20437)
                     /locus_tag="PC523_000893"
     CDS             complement(19685..20437)
                     /locus_tag="PC523_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDF2474448.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            complement(20457..21488)
                     /gene="rlmN"
                     /locus_tag="PC523_000894"
     CDS             complement(20457..21488)
                     /gene="rlmN"
                     /locus_tag="PC523_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDF2474449.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     EDMNNIPKSLRNKLEEISCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIKEILDACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            complement(21496..22821)
                     /gene="rsmB"
                     /locus_tag="PC523_000895"
     CDS             complement(21496..22821)
                     /gene="rsmB"
                     /locus_tag="PC523_000895"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDF2474450.1"
                     /translation="MDAREVGFKVLCDIEKNNNYSNIAINKHFKNLEISDIDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANSEKPSIYIRTNMLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEDSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLIKSTVNRLGLKNVCVERFDASEIDENSISKFDYVLADVPCSGLG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASRYVKVGGTLVYSTCTIQDDENINIVAS
                     FVEENHNFEFDSIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            complement(22883..23584)
                     /locus_tag="PC523_000896"
     CDS             complement(22883..23584)
                     /locus_tag="PC523_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDF2474451.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            complement(23653..24405)
                     /locus_tag="PC523_000897"
     CDS             complement(23653..24405)
                     /locus_tag="PC523_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HDF2474452.1"
                     /translation="MTDIKKYLTSVGILILILGLLIGVVNFFIISLTQIFSLIINIII
                     ISLILIILCSTAVTYRVIKGKNINSNIMKINFNIVSGLFPIISFIASSFGMSKSEIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCEECGRCNVGELI
                     KLKEKTNVKIFVATGGTLARKIIIDNKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMIDIEKAIEFLTGKNILACN"
     gene            complement(24415..25344)
                     /locus_tag="PC523_000898"
     CDS             complement(24415..25344)
                     /locus_tag="PC523_000898"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDF2474453.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDTIKSLNPDVIVVVAFGQILPKGILEIPKFG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDAGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            complement(25359..25799)
                     /gene="def"
                     /locus_tag="PC523_000899"
     CDS             complement(25359..25799)
                     /gene="def"
                     /locus_tag="PC523_000899"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF2474454.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHELDHLDGILFVDKIEK"
     gene            complement(25817..28342)
                     /gene="priA"
                     /locus_tag="PC523_000900"
     CDS             complement(25817..28342)
                     /gene="priA"
                     /locus_tag="PC523_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDF2474455.1"
                     /translation="MNSILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSEVI
                     RIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIESKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQKAVDEIKSNMFLDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKYGEYKLINIKSRANDKPLPNIEVVDMKEELDKGNRSIFSL
                     KLQKEIRCAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DDILNKFKDKKADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVVKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNISVSIDINPNSVL"
     gene            complement(28581..29780)
                     /gene="coaBC"
                     /locus_tag="PC523_000901"
     CDS             complement(28581..29780)
                     /gene="coaBC"
                     /locus_tag="PC523_000901"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDF2474456.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVNPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GAGKLANPDIIVEEVLANLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAISRGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILVGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(29785..30051)
                     /locus_tag="PC523_000902"
     CDS             complement(29785..30051)
                     /locus_tag="PC523_000902"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDF2474457.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKAKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(30054..30671)
                     /gene="gmk"
                     /locus_tag="PC523_000903"
     CDS             complement(30054..30671)
                     /gene="gmk"
                     /locus_tag="PC523_000903"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDF2474458.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKSAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(30701..31582)
                     /locus_tag="PC523_000904"
     CDS             complement(30701..31582)
                     /locus_tag="PC523_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDF2474459.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(31586..32416)
                     /locus_tag="PC523_000905"
     CDS             complement(31586..32416)
                     /locus_tag="PC523_000905"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDF2474460.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKINLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDSEFKVTSMLM
                     GVPHTVVIVDELDVEDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            complement(32703..33737)
                     /gene="ltaE"
                     /locus_tag="PC523_000906"
     CDS             complement(32703..33737)
                     /gene="ltaE"
                     /locus_tag="PC523_000906"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDF2474461.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDENGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVGLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGSKDFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            34033..35808
                     /locus_tag="PC523_000907"
     CDS             34033..35808
                     /locus_tag="PC523_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HDF2474462.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTIKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDAISKDLFIKNLEESEESIFKSIYSKFLGISPIIAKEICYRAG
                     INQNTIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIVIDKKVDRVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNNVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFMSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(36001..37653)
                     /gene="abc-f"
                     /locus_tag="PC523_000908"
     CDS             complement(36001..37653)
                     /gene="abc-f"
                     /locus_tag="PC523_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HDF2474463.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYIYLTESYSYISQSENNIEICSDSKEKSLLNAPDRFEEYLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEEMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRAYFEYQEYVKEKKRLEKALLHKKSLS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKASIEMKIK
                     IQNGMEIISKNLVEIKDMTLKLGNKLLLDNVSFKIKRGKKIALLGDNGCGKSTMIKEI
                     LNNKNDNIKINNKVKVGYFDQHQSLLDEAKSVLYNTKVNSSFDESFIRTNLNLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLIFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDTKIREFDCNYDEYIINRNKKNPSRESKIKKENLLV
                     LENRLITIVSMLSIEKDGLKKELYESEYNELLKQINKLKNSF"
     gene            complement(37941..39149)
                     /locus_tag="PC523_000909"
     CDS             complement(37941..39149)
                     /locus_tag="PC523_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDF2474464.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKIVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFNMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILGLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAFILLIGLGTTYLSNKGISIGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEE"
     gene            complement(39432..39965)
                     /gene="pyrR"
                     /locus_tag="PC523_000910"
     CDS             complement(39432..39965)
                     /gene="pyrR"
                     /locus_tag="PC523_000910"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDF2474465.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDVTLYRDDLEKIDVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(40324..41271)
                     /locus_tag="PC523_000911"
     CDS             complement(40324..41271)
                     /locus_tag="PC523_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2474466.1"
                     /translation="MFYFLKVKVGDLMYEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKIEKAKYLVKEEDNIVIEIPEPKVLEVVPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKENKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRAKLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(41240..41686)
                     /gene="lspA"
                     /locus_tag="PC523_000912"
     CDS             complement(41240..41686)
                     /gene="lspA"
                     /locus_tag="PC523_000912"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDF2474467.1"
                     /translation="MLYILIIILLIGLDQLSKMWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHISGKLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRIIWEYVFNVADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(41845..42624)
                     /locus_tag="PC523_000913"
     CDS             complement(41845..42624)
                     /locus_tag="PC523_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2474468.1"
                     /translation="MKKSIWGICICLLSIVGVYTYNHNTHNQDDLKEAYSLSENKISD
                     YEDVIIKKGSDDEKVIALTFDDGPDEVFTPQVLDILKKNNVKATFFVVGEKVEYNKEL
                     LKRQYTEGHEIGNHTFTHINVAKNSYDKVEKEITDTQNAVKNVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKKMNIILWSNLDPRDWSNPGVGNIINTILTKVQNGNIILLHDYNNRRN
                     DKSQTIQALEVVIPKLKEKGYKFVTISELIQHLDKNKQVQK"
     gene            complement(42673..43155)
                     /locus_tag="PC523_000914"
     CDS             complement(42673..43155)
                     /locus_tag="PC523_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF2474469.1"
                     /translation="MDTKKYKDKLLKERESLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGTCDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(43421..44842)
                     /locus_tag="PC523_000915"
     CDS             complement(43421..44842)
                     /locus_tag="PC523_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF2474470.1"
                     /translation="MVSFLGSIVVLIVGYFIYGTFVEKVFGINDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGIYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(44988..45758)
                     /locus_tag="PC523_000916"
     CDS             complement(44988..45758)
                     /locus_tag="PC523_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2474471.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSSIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYENYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSERSDIELSSGNSNLYKYKKETVNQDIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNNDYEVTVSRSKVKEFRLLMHI"
     gene            complement(45763..46965)
                     /locus_tag="PC523_000917"
     CDS             complement(45763..46965)
                     /locus_tag="PC523_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2474472.1"
                     /translation="MKASILITLIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGAVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
     gene            47846..48592
                     /gene="cdtR"
                     /locus_tag="PC523_000918"
                     /pseudo
     CDS             47846..48592
                     /gene="cdtR"
                     /locus_tag="PC523_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906004.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
     gene            48982..50373
                     /gene="cdtA"
                     /locus_tag="PC523_000919"
     CDS             48982..50373
                     /gene="cdtA"
                     /locus_tag="PC523_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylating binary toxin enzymatic subunit
                     CdtA"
                     /protein_id="HDF2474473.1"
                     /translation="MKKFRKHKSISNCISILLILYLTLGSLLPNNIYAQDLQSYSEKV
                     CNTTYKAPIERPEDFLKDKERAKEWERKEAERIEQKLERSEKEALESYKKDSVEINKY
                     SQTRNYFYDYQIEANSREKEYRELRNAISKNKIDKPMYVYYFESPEKFAFNKVIRTEN
                     QNEISLEKFNEFKETIQNKLFKQDGFKEISLYEPGKGDEEPTPLLMHLKLPRNTGMLP
                     YTNTNNVSTLIEQGYSIKIDKIVRIVIDGKHYIKAEASVVSSLDFKDDVSKGDSWGKA
                     NYNDWSNKLTPNELADVNDYMRGGYTAINNYLISNGPVNNPNPELDSKITNIENALKR
                     EPIPTNLTVYRRSGPQEFGLTLTSPEYDFNKPENIDAFKSKWEGQTLSYPNFISTSIG
                     SVNMSAFAKRKIVLRITIPKGSPGAYLSAIPGYAGEYEVLLNHGSKFKISKIDSYKDG
                     AITKLIVDATLIP"
     gene            50426..53056
                     /gene="cdtB"
                     /locus_tag="PC523_000920"
     CDS             50426..53056
                     /gene="cdtB"
                     /locus_tag="PC523_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylating binary toxin binding subunit
                     CdtB"
                     /protein_id="HDF2474474.1"
                     /translation="MKVQMRNKKVLSFLTLTAIVSQALAYPVYAQTSTSSHSDNKKEI
                     INEDILTNNGLMGYYFTDEHFKDLKLMAPIKDGNLKFEEKKVDKLLNKDKSNVKSIRW
                     TGRIIPSKDGEYTLSTDRDDILMQVNNESTISNTLKVNMKKGKEYKFRIELQDKNLGS
                     IDNLSSPNLYWELDGIKKIIPAENLFLRDYSNIEKNDPFIPNNNFFDPRLMSDWEDED
                     LDTDNDNIPDSYERNGYTIKDLIAVKWEDSFAEQGYKKYVSNYLESNTAGDPYTDYEK
                     ASGSFDKAIKTEARDPLVAAYPIVGVGMEKLIISTNEHASTDQGKTVSRATTNSKTES
                     NTAGVSVNVGYQNGFTANVTTNYSHTTDNSTAVQDSNGESWNTGLSINKGESAYINAN
                     VRYYNTGTAPMYKVTPTTNLVLDGDTLSTIKAQENQIGNNLSPGDTYPKKGLSPLALN
                     TMDQFSSRLIPINYDQLKKLDAGKQIKLETTQVSGNFGTKNSSGQIVTEGNSWSDYIS
                     QIDSISASIILDTENESYERRVTAKNLQNPEDKTPELTIGEAIEKAFGATKKDGLLYF
                     NDIPIDESCVELIFDDNTANKIKDSLKTLSDKKIYNVKLERGMNILIKTPTYFTNFDD
                     YNNYPSTWSNVNTTNQDGLQGSANKLNGETKIKIPMSKLKPYKRYVFSGYSKDPLTSN
                     SIIVKIKAKEEKTDYLVPEQGYTKFSYEFETTEKDSSNIEITLIGSGTTYLDNLSITE
                     LNSTPEILNEPEVKIPTDQEIIDAHKIYSADLNFNPSTGNAYINGMYFTPTQTNKEAL
                     DYIQKYRVEATLQYSGFKDIGTKDKEMRNYLGDPNQPKTNYVNLRSYFTGGENIMTYK
                     KLRIYAITPDDRELLVLSVD"
     gene            complement(53708..54709)
                     /gene="trpS"
                     /locus_tag="PC523_000921"
     CDS             complement(53708..54709)
                     /gene="trpS"
                     /locus_tag="PC523_000921"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDF2474475.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANAIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(54732..55430)
                     /locus_tag="PC523_000922"
     CDS             complement(54732..55430)
                     /locus_tag="PC523_000922"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDF2474476.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     misc_binding    complement(55473..55713)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(55804..57207)
                     /locus_tag="PC523_000923"
     CDS             complement(55804..57207)
                     /locus_tag="PC523_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2474477.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKINMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
BASE COUNT        19601 a         9566 c         6413 g        21823 t
ORIGIN      
        1 tatgataata agaaattctt caatatgtgt aaggcaaata tatatttcta ttgcaaattt
       61 ttgttattta tgataatata aatatgtttt tagtctttaa atagttatca ttttaaggag
      121 gtgtgcgtag atggcaaaag tatgtagcgt atgtggtaaa ggtaaggttt ctggaaacca
      181 agtatcacac tcaaataaac ataataaaag aacatggtca gctaacttaa gaagcgtaag
      241 agcgattata gatggagctc ctaagagagt aaaagtttgt actagatgtt tacgttctgg
      301 taaaattgaa agagcttagt aaccacgttt aaaagcctac ttccgtaagt aggcttttta
      361 atttaatcat ttttactctt tctcataaat tttaggatta tacttgaaat ccattttggt
      421 aattttattg ttactacttt catcaatact caccccacac tttatagtta aaactatttt
      481 aacaaaaaaa taataatgct ccacatccaa taagtattaa tgcggtaagt gctgaccaaa
      541 tccagccagg aagcaacatt gaaagaatta cagccattcc tgttgccaga caaagtattc
      601 ctagcaatac cttagacttg ttatttctca tggaacacct ccacatagaa cctgtatcta
      661 taaatatttt catattcaag tttatgcgac agttttccat tttagaccaa taaacatatt
      721 atgtagtttt aatttttcat actagaatta ttactgaact ccgatataga ttctacaata
      781 tccaaaattc ctttttttct gcttaacctt tcaggtccac tatataaaac gactggtata
      841 actttaggaa gcttaaaatt tttatcttct atttcttcaa gacttttttt cataataaaa
      901 ctcttccaaa tctgttctct atacgatttt aatcttggta caatttcagt atccatttct
      961 gtttgaaact ctaatactat ataatgtgat actttaccat ttttagaaaa cactttataa
     1021 attacatcaa cttcttttcc tttatattca catataaaag atgtttctaa ttctatattt
     1081 ttaagagtta attcctttgc gattgagaca cttgtaaaat tttgaatcaa acttaaaaaa
     1141 ctttctttat ctgaatacat tcttctatac tcttctcgtt tttcattgct tctttttaaa
     1201 ttcatacagt tcctcctaaa ctatagacct tctatatttt aataaaaata gtctataaat
     1261 tttatttttt aattttcact agtgagtatt gacacttgtg tgtaaaatat ccagtaaaga
     1321 tattttttta tatttttatt aaaagatata aatttttata ttttaatata ttgacattta
     1381 cgttgcgtaa aagtgtatat ttagtttata ggaggtacta attatggaat atacagttca
     1441 aaaattaagt aaactagctg gtataagtac taggacactt agatattatg atgaaatagg
     1501 tcttcttaag ccacttaaaa taaattcttc aggttatcgt atatatggtc aaaatgaagt
     1561 taataaactt caacaaatac ttttttatag agaattagga attaacttag aaaatataaa
     1621 aaacattata aattcaccaa acttcgatag tttaagtgcg ttaaaaaaac accataataa
     1681 attactagtc aaaagaaagc aaattgattt actaatagaa aatgtaacta aaactatagc
     1741 tctgaaggag ggaaaataca ctatgacaga tatagaaaaa tttgaaggtt ttaaagaaaa
     1801 gatgattgat gaaaatgaaa aaaattatgg aactgaaatt agagagaatt atggtaaaga
     1861 tattatagtt gcctcaaata aaaaatttaa aaatatgtct aaacaagatt atgaagattg
     1921 gcaaaatcta agcattgaaa taattagtaa gcttaaaaaa gcttttaaaa ctggtgatgc
     1981 ctccagtgaa cttgctcaag aagttgcaag attacatcat aaatggttga gctacacttg
     2041 gagcacatat tctaaggaag ctcatgcctc tttagcacag atgtatgtat gtgacgaacg
     2101 ctttacatct tattatgata aagaacagcc tggactaact aagtttctga gggatgctat
     2161 agtagtatat acaagagata attaatataa aataataaag aggatgtctc aaaatgaact
     2221 ttttagttca tgagacactc ttttttatat gaaatcaaat atgtttttat catttcaaca
     2281 ataaaaaaag gattatctct attttgagac agaccctcta ttgtatctaa ttacaatcag
     2341 tataaagtaa tagaaaataa tataaaaatt actcattact atttatatta tcaacaaaaa
     2401 attgttctat ttatttttta taaaacttat gtaatataaa attttatatt atagacttga
     2461 ttatggaaag aaaactatca aaatcattac aattagatat actatctcta atatcattgt
     2521 catgaattat ctttaaaagg atatcataaa tggagtttat ttttatataa tcatctttat
     2581 ttatagccaa catcatgatt aatttaacag tagaatcttc actccaatat attggttcat
     2641 tcaatatgga aatacaaatc ttagttttta tagagcttaa ttccatagga tgaggtatag
     2701 ctaaaaaatc atctatattg gtagaagata aactttctct gtagaataca gattcagtaa
     2761 atgatggaat tacaatttca ctttgagtta atttgttgca catcaacttt ataagctcat
     2821 ccttctcttt gattttagga ttaacaataa ataaattttt atcaaagaaa ttgtccaaaa
     2881 gattactata aatacgagaa ttattagtga ttgacttaga tatattctct atatcttttt
     2941 ttaatagttt taaatctact aatacaatag gaatactatc atgatttaaa ggaattgtcg
     3001 atattattat atctacatct gacaattcac tagctaaaaa ttgattaaat gatagacatt
     3061 gaagtatatt tattttatca tgaaaaactt tgtttaattg aacctctaat aacctacttg
     3121 aaccataccc tgaaccacat actaatacta cgtttttaca cttaatattg ttttgaaaaa
     3181 atctctctat tccagcacct atatgtaggg atacatatcc aatttcatct tctgttaaag
     3241 agtaaatact atttttaaaa actttttcta tagcatttaa acttatttca aatgctaaag
     3301 gataattact ttttattgta tttataaggg gattcacctt atttaaatta taagatttag
     3361 aattaagaat tgatttaaag tgcagtacta aatcatcaaa taatacacta tcatttctaa
     3421 ggtcaaaagt atattgattg ttgattactt ctaaaatttc ctcaacatac tctataattt
     3481 tggtatctgc cctattggaa acgttatcca aaagagaact tttagttata aaatgattat
     3541 ataaataaac tctgtctgcc tttgaaataa ttatagtata tttactctca atataattaa
     3601 atatattttc aacagccgta ttcgattgaa aatctgttaa aaccacatct aaaacattat
     3661 ctaagcagta accacctagt attctgctta tagtaataga taaatgaata ataaaatttt
     3721 ttagattaaa atctaagaaa ttgatattaa atttattaat ttcacttagc actatcttct
     3781 gaagctctat taaatctaca ttattaaata attctatttc tctatctgta attccaatta
     3841 tataattttc aaaattccta tttatcatat tattagaaat acatcttcga atatctattt
     3901 ccttcccttc aaccttaact ccataatatg gcttagaaac taatattaaa ttatactttg
     3961 acaaaatagg ctttattctt tttatatcat ttagtattgt aaatttactc acatacaagc
     4021 tattagctaa atcttctagt tttatatatg tattgttata aagaatatat tttaaaatat
     4081 attgatttct ttctatagga gaatctggta tttcacttga atccataata tcatcagata
     4141 ttaatgctag ataatgttgg tataatgcta aattaagaat atctatataa tatccttctc
     4201 ctttttttat tttaataata gcaccatttt tctctaaagt ttcatttata gaagtaatat
     4261 catttcttat agttctctca ctcactttta aatgattact taaagtttta caagtaattt
     4321 tatcatcact ttcatttaat agtttaaata acgtgagcaa tctcttgtgc ataaatatcc
     4381 ccccttatag aatttcttct aaattatact atttacactc atataataca attactctaa
     4441 taaaattgta ttatcataat ttatttccac ttcgacatgg aaattagtta ttatcataat
     4501 ataaatttcc atgtcgaagt ggaaattagt tatatttaat ttaattagga taacgattac
     4561 aatataaata taagttaaga cattacgaat ggagtggaaa aaatatatgg tactacaaga
     4621 aaagaatata tttatagata aagacttaaa aagcaaagag gaagtattaa attttattgc
     4681 agacagtgct tatatactag gtattacaac taataaagat gctcttttaa aagaccttct
     4741 aaaaagagaa agcgaatttc caacaggaat aagagatgga tttgcgatac ctcatacaaa
     4801 aagtgcaaat gtaaaagaag cttctatact ctatattaaa acaactaacg agttagagtg
     4861 gaaaacttta gatgatagta aggttagata tgtatttagt ttgttagctc cacatgaaaa
     4921 tgaaggaaat atacatttga aaactctatc taaattagcc acatgcttaa tggaagatga
     4981 ttttataagt gaagttaaaa atgctagtga tgcaagtcat ttagtaaaat atataactaa
     5041 taaaatgaag gaggaataat tatgaaaata gtagcaataa catcatgtat agcaggattg
     5101 gcacacacac ctatggcagc aaaagctttg gaacaagctg catcaaaaat ggggcataaa
     5161 atcaaagtag aacaacaagg tgctatggga cgagtaaata ctataacaaa agaagaagca
     5221 caagatgcag actttatact tatagcatct gaccaaacta tatctggaat ggaaagattt
     5281 gatggtaaaa aagtaataaa agttaaaata ggtgtagcat taaaaaaacc agaagctgtc
     5341 ttaacaaagt gtatagaaac tatttcttaa taaaaagaat ctcactctaa tctagaatga
     5401 gaaaaatcaa aaattaaaaa attatctaat gtaattataa catattatca aaaaatataa
     5461 caaacttata taggaggatt aaaaatgagt aaaaaaaata actctctttt aagtgaactt
     5521 aaaggacact taatgacagg tatttcatat atgttgccac ttgtaatagg ctcatctcta
     5581 gttgtagcta taccaaaact tacagcttta gcaatgggta taacaagttt agatgcttac
     5641 aaaaatgtat ccggttttta tcatattctt tacttaatgg aacaagttgg ttggactggc
     5701 ataggtctat taaatactgt attagcagga tttatagcat tttcaatagg tgaaaaacca
     5761 tctatgggtg caggttttat tggtggactc atagcaagta atactaatgc aggattctta
     5821 ggagcagtaa cagcaggttt ttttgctgga tatatttgca aatttttaaa gaaaaaaatt
     5881 aaaatatctg ggtcggcagc aggaatgatg ccacttataa taatgccact tataacagtt
     5941 ggattaacgg ggtttttaat gagtgttgta ttagcagggc ctttaggaaa cataaatact
     6001 tcattaaatg cttgggtagc tgaaatgtgt caaaatggaa caaattcggt ggtattggct
     6061 ttaattttag gttcaatgat aggatttgat ttaggaggac cggtaaataa agcagcttgg
     6121 atggcaggaa atgcattatt gttagagggc atctatctac cagcaatatt ggtaaactgc
     6181 gcaatagtta tccctccact aggatatggt ttagctacat tcattagaaa atcaagattc
     6241 tctaagacat tatctgaaac aggaaaaggc aacatagtta tgggtataat tggaataaca
     6301 gaaggtgcaa ttcctttcac tttaaaaaat ccattaaaat tagttcctgt caatatgata
     6361 ggatgtgctt tgggtgtagg attatcagct ttattaggtg tacatgctat aatgcctcca
     6421 gttggtggat tatatggatt tatatcagta ggttctggat gggcttactt agtaggtgcg
     6481 atatttggag ctttagtaat agcaatatta tcaacaattt tagtagactt taatgaagat
     6541 gaaactgcgg aaaatacaga atttgatgaa atagagttag atataatgta aattaaagat
     6601 ttaattttag ctagttgaga attgagcgta ctctcaattc tcaattaaat ttagggagga
     6661 ttttatgaag aagacaaaag tacatgtagt tccacatagt cattgggata gagaatggta
     6721 ttttacaaca tcaaggtcaa aaatatatct aatgaaagat tttcaagata tattagacat
     6781 attagaagaa aaagaagact ttaaatactt tactatggat gcacaagctt ctctcttaga
     6841 tgattattta aaatggagac ctcaggatga atatagaata agagatttag ttcaaagaaa
     6901 aaagttagct ataggacctt ggtatactca aacagaccaa ttggttatat caggagaatc
     6961 tatagttaga aacctttact atggaataga tagatgcaat gaatttggcg aacctatgag
     7021 ggttggatat gtaccagatt cttttggaca atcagctcaa atgccacaaa tatatagagg
     7081 ttttggaata gatagttcat tattttggcg tggagtttct gatgatatgg tagatacaac
     7141 agaatttata tggaaaggtt ctgatggctc aaaggtatta gcagttcaaa taccatttgg
     7201 ttattactat ggaggtaata taccagaaga tgattcagat ttaaaagaat atttaaaaga
     7261 tacaattgga aaacttaagg ttaaatcctc tactaataac gtatatttcc caaatggatt
     7321 tgaccaagca ccaataagaa aaaaccttcc tgacatatta aaaaaagcta ataaaataga
     7381 tactgaaaat gaatacgaaa taagtgatat agaaaactat gtagattcag taagaaatga
     7441 aagaaatgaa tttacagagc ttaaaggtga atttgtaaat gctaaacata tgagaataca
     7501 caaatcaata ttctcctcaa ggtcagacct taagataatg aataataaat tagaaaatta
     7561 tattacaaat gtacttgaac cattattaac attaagttat tcacttggaa atgaataccc
     7621 tcacctaatt ttaaaagata tatggaaact tatgttcgaa aatgcagctc atgacagcat
     7681 aggttcttgt aatagtgaca caacaaatga agatgtatac acaagataca aacaagccag
     7741 agatttgagt ataaatttgt tagattttca tatgagactt ataagtactc aaatcaaaaa
     7801 taataatgaa gaaataacat taacagtatt caatggatta ccagaagcta gaaatgaagt
     7861 tgtagaattt gatacttata ttccaggtca agattttgtg ataaagaaca aaaatggaga
     7921 aattcttaat tatataataa aagaaaaaga agatattaca gattatatat taactcaaac
     7981 aataaaatta aacccaagta aaaaaatata tataccaaaa actgtatata gggcaaaaat
     8041 tgcactttca gtaaaaaata taccacccat gggatatact cagctaattg ttgaattaga
     8101 tactaaaaat aaatgtgaaa taaaaacaag tagttgtaaa aaaatagaaa acaaatacta
     8161 taaaataaca gccaataaaa atggttcagt agaaatatta gataaagagt ctaataagac
     8221 ttataaaaac caagctgtaa ttgaagataa tggagatgat ggagattcat acaactattc
     8281 gcctccaaga aaagatattt atgtttcatc tttagattct gtatcttcag tagaagtgct
     8341 aaaatcaaat gttcaagaag aaatgacact taaatattca ttgaccatac cagcttcact
     8401 agaagaaagg actataggta aagtttctgt gaaaatgcca gtaacaatga aaatcacctt
     8461 agaacctaaa gagcaaataa ttaagtttaa tattaatata aaaaatgacc aagcattatc
     8521 tcatagagta agagttttat ttaatacgga aatagcatct aagttttcta tagcagatca
     8581 acaatttgga ataatacaaa gaccaacagc attagaaaaa gaccttgcat tatggaaaag
     8641 agacaattac tcatggcaag aaaagcctat aactattgaa ccaatgcaaa gttttgttac
     8701 tttagaagat ggacaaaaag gagtagcatt aataactggt tgtgtgagag aatatcaaat
     8761 agttggtgct aatttagata caatagcatt aacactattt aggtcatttg gatatatggg
     8821 aagagaaaat ttactttata gaccaggaag agcttctgga gaaaaaataa tagcaacacc
     8881 agatgcacaa ttactcaaag agttaaactt taactttggt ctttacatct acaacaataa
     8941 atttgataaa tcaaatatag ctaatactgc taagagattc ttaacaccta tacaaatata
     9001 tgaatatgct gattttttaa atggtagact tatatttgta tttaatgatg aaaaacaaat
     9061 atatgaaaat gaatatagtt ttatgaatgt aaataattca aatttcacag taagtgcaat
     9121 aaagaaagaa gaaaaaggag acggaattgt agttagaatc ttcaatggta tgaaaaatga
     9181 ggattctaaa ggaagtctta acatcaataa aaatatcaat gctgcttata atgcaatgct
     9241 tgacgaaatc tatgataata ctaacaagct taaggtaaat actaaaactg tagaagtaaa
     9301 ttctttgtca cattgtaaag tacaaacaat agtaattaaa taatataaaa tagcttatac
     9361 aagatttcaa aagaaattac taaccttatg ttaatggtaa atccaaattt acactattta
     9421 aaatatctat aacactaagt aacattttat aaaataaaaa ggtgaatctt atatattagg
     9481 attcaccttt tttaaataaa tcaacattct attttaaaat aagatttaca agttgttttg
     9541 aaataatgtt taaaaactac tttaaaacaa tacctataaa atattatttt atggatttta
     9601 taattattaa agaagtctat tctaattttt attaaaagta attgtaactt taaataaatc
     9661 accatccaca cttatattca tcttaccacc ctgtaattct ataaaacttt ttgctattgc
     9721 taaaccaaga ccagaacctt ctgtgttgcg tgacttatct cctcttacaa atctttccat
     9781 tatatcatct acactaaaat caatctcagt tgcagacatg tttttaagca taatacttac
     9841 tttattgtct gtttctaaaa tatcaatata cactcttgaa tttggcattg catatttgct
     9901 tatatttatt ataagatttt caaatactct aaatgtacgc tgactatcta atggtaatat
     9961 tattttttca ttagaaaagt tattctttac tattagagat gaatctgcaa gtttgtcatc
    10021 taattctaaa agtgtttgtt tcattagaga cacaatatcc acattcatta tgttcaaatg
    10081 aacatctcca ctatttacct tacttacttc gaataagtct tctattagat gttgcaatct
    10141 ttgtgacttt ctatctaatg tatcaatata caacttacga ttttcatctg ttatactttc
    10201 atcttttaat aaatctatat aagtgattat agatgtcaga ggtgttttta ggtcatgtga
    10261 tacgtttgat ataagctccg ttttcatctt ttgactctta acctcttcat caacagcttt
    10321 tttaaatcca gcttgaatat ttcctaattc atcttttata ggattaaaaa atcctaaatc
    10381 tctttccata tttacttcta agtttccatc tgaaattttc tttgtaacat ctaaaagttg
    10441 attatattca tatattgtct ttttagagta atctgacaat atcttaaata atactactga
    10501 atatatcaat gcaagtatta ttccaaagaa ccaaaaacta cacattataa ctataattaa
    10561 taagtttatt cccaaaatta tagctatagt tttattaggc ttttctttta aatctgttat
    10621 tctaatttga ttaataacta cttttcctga ttttatcaac catacaaaaa acattcctgt
    10681 gacagtattc tttataaaat actcttttat tccaacattt aaaatatgtt ttaaaagtat
    10741 tataaataca aatattattg agaatattac aaaccaataa gttagattca ttaaacttac
    10801 caatatacta gccattttag cacttatgtc attgtttatc agatattcta cataatttcc
    10861 actcaaagta ttacttatta tactagtagg atataaatct acaattcctt gtaaaatagc
    10921 aagaataata aataatatct ctaatggtat tcttgataat gtatcaaatc ctagtaattc
    10981 tttagatttt ctatatggga atgctaaagc tacaatcaca actatagcta atataatttt
    11041 taaaatcatt ccagaaatct tatagtaaga attattttct acattattta tatatgaact
    11101 tatactatca ttattataaa tatttccata actatactct ccaccatccc ctttacttat
    11161 taccttaggt actgcatata ctaatgtcac gttttttatt ggagatagct tatatgaaat
    11221 tggatttcca cctaaagttg tattcattaa atcttctctt gctttagaca ataacatttg
    11281 actaataact ttttgattta ctccataaga atccaaaaca tttacttttc catctttatc
    11341 aaaattcatt acaatgtaga atctatagtt ttcctttata tctttaatct gcttttgtct
    11401 ttcctcatca ctcatttctg tattttttga tttattttca tcagaatctt cttttgaagc
    11461 ataattcttt gttgtatcaa gtgctcctaa ttctttatta tcgttaaaaa attcaaagtt
    11521 actttcttta tcaactaaat aatattttaa attttttcct gattctttaa taaaagactc
    11581 ccaattttca aagttttgat taaaatctgc attaatacta tcatctgtat aataagtctc
    11641 tgagccatca taagtatctt tttctttact tacatttttt atatagttca tagtttcttt
    11701 acttataaaa agttttgagg gttgtatcat cttaccttct tgttcttctt tcatgttatg
    11761 atataaaaca taacttcctt tatacatatt atctacaaaa ctttcctgtt caaatggatt
    11821 ctttgctcca cctttagccc ctgcttgaat agtagaatat tgactaatca tacctaatga
    11881 tgctgataac ataagtataa aaactataat agtaactata tatttatctt tgaatttttt
    11941 caattttgta tccaacaccc cacaccacct ttaaatattt aggatttttt gggtctactt
    12001 ctattttctc ccttatattc ctaacatgta ccataactgt atctgtattt acagcattat
    12061 cattccaaac tctttcatat atttcatcag cagaaaaaac tcttcctgca tttctcataa
    12121 gtaagcttag tattttaaat tctatgggag ttgcttttat atgcttccca tcaacactaa
    12181 cttcttttgt attttcattt aattctaatc cacctattgc aaacacgcca tcatcatcat
    12241 ctactttttg ctctttattt tcaaccatat ttaaatactt agtgtatctt ctaagttgtg
    12301 agtttactct agccaatagc tctaaaggct taaaaggttt tgttacataa tcgtctgccc
    12361 ctatattaag ccccattatt ttatcaactt cttctgattt tgcagatagc attattactg
    12421 ggaaattatg attttctcta agcttcattg taagtgttat tccatccatt ttaggcatca
    12481 ttatatcaat tattgctaaa tgtatttctt gattttctat aaccttaagt ccttctaatc
    12541 cattgtatcc tttaaaaaca ttatatcctt gatttaatag gtatatctct atagcatctg
    12601 ctatttcctt ttcatcttct accaccaaaa tattatatga attcatttta atctccttta
    12661 taccaattaa tttataaatt ttctttacat atatgattat acaatatgat agaagttttt
    12721 ccattttttt atatatttat atctttctaa tagttatttt tatttaatgt acaccttaga
    12781 gacctccctc tagtataaat ttagtttttt cattttatat acttaatatt aaagttaaaa
    12841 acttaagata ttacatatac ttttctaaag attttctaaa gatttggagt tattcaaaat
    12901 taatatgcac aaaaaaggta gtttttattg cattaattaa atcaatgatt tgaaacataa
    12961 acttaatatc aataaaacct acctttttat ttttaactat ttatttttaa ctcattttga
    13021 atcttaaaaa ttattgtctt ttattttata aatgtacata tttcttctac tggtttttta
    13081 gatgggctct tcaaattatc ctctggcact ccaagagcca acattgcacc taaaaaatac
    13141 tctccttttt caaatccagt ttctgcttct aaaacagctt ctatttcatc tgctgcgtag
    13201 ttttgacttg tcaaccaaca agaaccatac ccaagttcaa ttgctgataa tgtaaagttt
    13261 tcaatcgcag cacctaaact ctgcatacct ggatttctta tgaataattt atctatagtt
    13321 tccttaggtg catctataag ctctaattca taatatcctg atggattata tacctttgta
    13381 aaaactaata ctaaaactgg agcttttaag taaaatagtg taaagttttt aacaaatttt
    13441 ctaaatctat tagccttatc tactgatact ttttccattt ctacaagtat ttcttgttgc
    13501 tttttagtta tcacatctgc tatcttttcc attaagtcac gacgttttat aatgacaaaa
    13561 tgccagtttt gaatattctt tcctgatgga gctgcacctg ctgcttttat catttttaaa
    13621 atatcttcat ctgacacatc ttgattttta aactttctta cactttgtct tttaaaaata
    13681 gtatcctgta attccatttt taaatccccc cataactttt ttattatttt aatataattt
    13741 ttaaactcat cctattttaa tatatcatgt tctaacaaat ctttaaatga gtctataaaa
    13801 taatcatata catctacatt cccaaatcca tatttagcat atataaaagg gatacctgca
    13861 aacttagcac tatttgcatc accttgagta tctccaatat agattgggtt ttttaattta
    13921 tttctttcaa ttataagttt attattttcc cctttatgca aaccagtatt acctggacat
    13981 tcaaaatcta caaaatactg ttctagttta tgtgctttta aaaaagattc tatatatcca
    14041 gcttgacaat tacttactat aaataattta tattttttag atagtaattt aagagtattt
    14101 tctatatcat caaataattt agctccattt tcagataaat attcacattc tttcaaagaa
    14161 cattcattta ataaattatt tctttcttct tcacttaaat ttggaaataa acgatttgct
    14221 atttcctcaa aagaaagccc cataagactt ctaaataaat cttttgtgac acttaaatct
    14281 atttctttat gtttacttaa aacatcctgc caaactttgc atacttcttc agttgaatcc
    14341 cacaacgttc catctaaatc aaaaataata ctatctacca caaaaaatcc cccttatgtc
    14401 aaagtttatt atctatttat aattatatac taaaaagcag atacaatcat aaatatattt
    14461 tacttgattg tatctgctct acaatataaa atcaattgtc aaaaccaaat tttataaatt
    14521 tctaactaca agtacacatc cttgctctac tttaatctta caagaattac tcaacataac
    14581 atttgatatt ccaataggta cagagtatct catataataa ttatcgagag gatattctaa
    14641 tccggtaagt gttacaccct tagcatcacc ttttatagct attacagaaa tagtgtctcc
    14701 tggatttcca tacagtgaaa tttcttcatt ttcaactata tacatctctt ctttatcaga
    14761 aagtatccta gaatatatac catcttcttt taaataatat aatagcttta tatttgcaag
    14821 agtgtggtca atcctacctc ctaaagcacc aaaaaaatca atatgatttg ctttaagtgt
    14881 cttagctaaa aaaagacaaa gctctgtatc agtctcatct tttttagatg gaaatttttc
    14941 aaatttaaca cctttatttt tatagaagtt tatcttttct tcttctactg aatctaagtc
    15001 tcctaatata taatctggca ttatttccat tttataagtg tgatttgctc ctccatctgc
    15061 acatattatg cagtcataac attctttaat tataatacct cttgtaactt cgtaatcttc
    15121 tatttcacca tttaaaacaa tacagatttt cattcttaaa tttaaactaa ttcttcaaga
    15181 gaagcattct ttctaaataa attaactgtt tcttgaatgt catcacttcc aaatattgca
    15241 gaacctgcta ctattacatt agctcctgct tgtacaactt tatccacatt tttagggctt
    15301 ataccaccat caacttctat atcaatattt aatccttgac tatctataat agcttttaac
    15361 tctttaatct tatctaaaac acaaggtata aatgattgtc ctccaaatcc aggattaaca
    15421 gacattaata ataccatatc taaatcaggt agtacatgtt taatagtatc tattggtgta
    15481 gctggattaa gcactacacc tgcttttatt ccatgtgatt ttatattttg tatagttcta
    15541 tgtagatgaa cacaagcctc ttgatgaaca gttattatat cacatcctgc ttttacaaac
    15601 tcttcaatat actggtctgg attttctatc ataagatgag catcaaatac catatttata
    15661 ttttctttct tcaaactttt gactacaagt ggtcctaatg taatgtttgg tacaaagtgt
    15721 ccatccatta catcgatatg aagatattca catcctgcac tctctacctt tcttacatcc
    15781 tctaataatt ttgcaaaatc tgctgataat attgatggtg ctaatttaat catgtttcta
    15841 atacctccta ctattatttt gtcttatttc atttagtagc tgtatataac tctcatatct
    15901 ttctttagat atttctccat ttgcaacagc ttcttttata gcacaattag gttcattttc
    15961 atgaatacat tttgaaccaa acttacaatc attaaattta tcaaattcta taaaataatc
    16021 tttaagttca acttcctcta tatcttcaag tgcaagagaa ctaaagcctg gtgtatctgc
    16081 aaccattcca ccaaattcca attttaaaag ttcagcatga cgagtagtat gttttcccct
    16141 ttttatttta tcacttacca ctcctgtttg aagtttaaag ttttcatcta tttcatttag
    16201 taaacttgat tttccaactc cagatggtcc tgcaaacact actacatttt cttttaactc
    16261 ttcttttact ttctctatat ttaatttagt aatattgctt acaggaataa ctttgtatcc
    16321 actaagttca tatatttttt taacagtttc tagtgtatca ttatcatcta aatctgcttt
    16381 tgtaaggatt attacagttt ctaaattttc tttttctgcc aaaactataa atctatctaa
    16441 aagagataag tttggtttag gattttttat tgcgaatact attagtgcct tatctacatt
    16501 tgctatagga ggtcttataa gttctgtatc tctactgtct atttcttcta agataccttt
    16561 tttttcatcc tcatcaacaa tgcttatttt gactctatca ccaaccagag gtgttatttt
    16621 atgttttcta aatattcctc tagccttaca ttcataaata ccattatgcg tatctacata
    16681 ataaaatcca ctaatcccct ttataatttt tccttctagc atcaaatgcc tccctttata
    16741 aaatttatat aaacacatac tgtttatgag tagtatatcg tgttattctt tcctattttt
    16801 atataatacc acattttttt taaataacaa atcctccgtt tggactaatt acttgaccag
    16861 ttataaaatt tgatttgtct gatgccaaga aaatagcaca atttgctata tcttctggag
    16921 ttcctagcct cattagagga gtttcttcca caagaatatc tatatcttcc tcattatatc
    16981 cagataacat atcagtatta atgacacctg gagctatgct atttactcta atatttgatg
    17041 gtcctacttc tttagctaat gcttttgtca ttcctattat acctgctttt gttatagaat
    17101 aatgcacttc acaagaagca cctgatatac cccagattga agatatatta attatatttc
    17161 cttttttttc tgatatcatg tattttaaag caacttgtga acaataaaaa cttcctttta
    17221 aattaatatt catcatatta tcccagtcta catcagttat atctgtaaac aacttgtctt
    17281 gacttatacc agcattgttt acaagtacat ccaatcctcc aaattctttt atacaataat
    17341 ctaccatatt ctctacatct tctctatttg aaacatcagc cttaaaaagt tttatagaaa
    17401 aattttcttc atttaatcta gtatacaact ctttagcttc attttctgac ttattaaaat
    17461 ttactaatac attataacct tcttttgcaa aagctttaga catagctttg ccaattcctc
    17521 tagctcctcc tgtaattaaa actgttttct ttttcataaa tgcttttctc cttatattat
    17581 aaccaattca atattttata caaaacaaac tgccctttaa agaaaatatt ttctataaaa
    17641 ggacagctct tattttatta atatttattc tgttttacca atatttgcac cagtgcctga
    17701 tgaagttcct ccattatctc ctgagtttga gcctcctgaa ctatctccat tgtctcctga
    17761 gtttgaaccg cctgagctcc ctccattgtc tcctgaattt gagcctcctg agttccctcc
    17821 attgtctcct gaatttgagc ctcctgagtt ccctccacta tttcctgagt ttgaacctcc
    17881 tgagttgttt ccattatttc ctgagttccc tccactattt cctgaatttg aacctcctga
    17941 gcttcctcca ctacttcctg agtttgaacc aggttctgtt ggtgtatcat tatttggtgt
    18001 tgttggtgtt ttatttggta ctgttggagt ctgtgtattt gatttttctg aacctttact
    18061 tacatataaa cttatctcgt caccttcttg cactcttgat gaaccagctc ctggactctg
    18121 atttaaaact gttccttctt tatatattga actatattca tacttaacag ttcccacttt
    18181 taagttattt tcttcaataa gttgttttgc attacttaga gtaagtccca ctacatttgg
    18241 aaccgatgcc tgacttgaac ctttacttac aacaacattt atagtatcac cctttttgat
    18301 atttgtactt ccaccttcag gtgtctgtga tattatacaa ttttcatcaa cttcactatt
    18361 atactcttcg ctttttacac ttaggtataa cccttctttt tcaacagcac tttgagcttc
    18421 ttctaatgtc atatttacaa gattaggaac agtcaaagat ttattgccaa accctccaga
    18481 aaataaaaac ttatatgcta agaatacttg agcacacaat atcaatatca aaacagttgc
    18541 aattactttt aatcttcttc tagattttgg gctttcctgt tttttcttct ttttcttagt
    18601 tctcttactt ggagcacttt tagttctctg attcttttta gctctcatta tctcttcctc
    18661 ttcttcatct tcttcataaa aatcatcata gtaatcttca tcatcatcca aatcagctac
    18721 ttcaacaggc ttaaccactt tttcaggggc tggttttgca agtattggat ttacaacttt
    18781 attaatttcc ttttcatcaa ttttcttagt tgcaaaatca tcatattctt ttataaaatc
    18841 taaatcaata tttttttcta tatactctat atcttctatc aattcttcag cagtttgata
    18901 tctatctgca ctagattttt ctgtaagctt ttttattgta gttcttacac tttgaggaat
    18961 cctaactttt tcttctgatg taaagtctat atcatcatta atgtgttgaa gtgctataga
    19021 aattggactg tctcccctaa atggaacttt tcctatcaac atttcatata aaactatacc
    19081 tagagaatac aaatcagcat tattagttac aaatttacct ttagcttgtt ctggcgaaaa
    19141 ataatgcact gaacctatta tactaccaat attagtcatt gttgaatttg aaacagcttt
    19201 ggctatacca aagtcagcca cttttactac tcttccttca ttcgatatga ggatattatg
    19261 aggttttata tctctatgta taatcccctt tttgtgcgcg gcactaagtg ccatagctat
    19321 ttgttttgtt atatctaatg cagtatattc atctaatata ccttcatctt gtataatctc
    19381 ttttagattt tttccatcta caaactccat tacaatgtag tgaacctttc catcttcacc
    19441 aacatcataa acatttacta tatttggatg ggaaagactt gctactgcct ctgcctctct
    19501 tttaaacttt gttaaaaatt catcatcatc tacaaattca ggtctaagta ctttaagtgc
    19561 aacagtcctg tttaataatc tgtctttagc ctcatataca aaggccattc ctccatcacc
    19621 aatcttcctg atgatttcat atcgatttcc taaaattgta tctcccacaa tatcaccgtc
    19681 ctaattatac ttttatcact ataaccgaaa cattatcttt accagaaaca tcattggcca
    19741 tacttatcaa ttcttcactt atattactag ttctctcata ttcataatct aatattaaat
    19801 ctcttatatc ttcatcatca ataaacccag ttaatccatc tgtcgcaaga agtattatat
    19861 cacttttttt gatttctttc ttaaaaatat ctacaacaac catatcatct gtcccaattg
    19921 ctctagttat tctattcctc tgtggatgtc ttcttgcctc ttcttctgta atgacatgag
    19981 ccatcataag ttcttcaacc acggaatggt ctatagttat tttatcaaat ttttcatctg
    20041 ttaacaaata acatctgcta tctccaacat ttgccacata taagacatta ttatacacta
    20101 tagctgtaac taatgtagtt cccattcctt caaattctat atcttccata gattttttgt
    20161 gcacaattga gtttacatta ttataagcct gttttaatat atcatctata tagtctattt
    20221 ttacattatc atgttgtaat aagttttctt ttagaaaatc aattatattt tctactgcta
    20281 atttactggc gacttcacct tttttatgtc cacccattcc atcagctatg gcaaatattc
    20341 ctattggacc atgttctgta tctataactt ctccctcaca atagtcttca ttgttttttc
    20401 ttatttttcc tatatgggag gcacaactat aaaccatacg acttcctcct tacaaattac
    20461 ttgtattttc ttctcaattg cccacaagct ccactaatat cagaacccat agacatccta
    20521 actgtggcag gtatattgtt tttctctaaa ctatccctaa atttataaat aaatgcttta
    20581 tctggctttt cgtattctct ttcttcaact ttatttattg gaattaaatt tacatgacat
    20641 agcatacctt ttaggagctt agctaatgcc tttgcttctt tttctgagtc atttacccct
    20701 tttataagag aatattcaaa tgtaaccctt ctatttgtct tttttatata atatctacat
    20761 gcatctaata tttctttaat agaataagca tttgctacag gcataatttt tcttcgttct
    20821 tcatcatatg gtgaatgaag tgataaagct aaatttatag ctatttctaa atctgccaac
    20881 tcatacattt taggtactat cccacaagtt gaaagagtta tatgtctata ccctatatta
    20941 agaccatttt tttcgttaat tatttttaaa aattgttttg tattttcaaa gttatcaagt
    21001 ggttcaccac ttcccatcaa tacaagattt gataccctct taccagtatc ttcttgaatt
    21061 ttaataactt ggtctaaaat ctcccatggc tctaggtttc taattaaccc atccattgta
    21121 gatgcacaaa aattacatcc catcctacaa cctacttgat tagagacaca gacagtaact
    21181 ctactatcat aatccatcat cacagtttct attatatttc catcatctaa taaaaacaaa
    21241 tatttttttg tattatctac cttagattct aactttaatt ctatatctat atgacctata
    21301 catgaaattt cttctaattt atttctcaaa ctttttggta tattattcat atcctcaaaa
    21361 gtttttgcac ccttatatat ccaagagaat atttggcttc ctctaaaggg cttttcatct
    21421 atagttttca taaattcttt aagttcatct tcagtaaaat tttttaatac tatctttttc
    21481 ttttccattc tctcactacc ttattctctt taattttgct atgaaaaaac catctatacc
    21541 atgaatatta ggatatatct ttaaatatcc tttatcttga ttatctaaat ctacatttac
    21601 tgtatcaatc gaatcaaatt caaagttgtg attttcttca acaaaagaag caactatatt
    21661 tatattctca tcatcttgaa ttgtacatgt actatatact aaagttccac caactttgac
    21721 atatctggat gcattttcta aaattttctt ttgtatagaa gtgatgtctt ttaactcttc
    21781 ttctttctta tatttaatct ctggtttacg tcttattata ccaagaccag agcaaggaac
    21841 atctgctaaa acataatcaa atttacttat actattttca tcaatctcag aagcatcaaa
    21901 tctttctaca catacatttt taagtcccaa tctgtttaca gtagatttta taagttttag
    21961 tttatgctca aaaatatctc tagctactac ttgaccagtg ttgttcatta aagtggctaa
    22021 gtgagtagat tttcctccag gagcactaca tacgtccaaa acaaatgagt cttcttttgg
    22081 attaattact ttccctacta tcatagaact tatatcttga attgtaaata aaccagcctt
    22141 aaataattca tttttttcta tatttttcat tttttcgact ttaatagctt cttctaccat
    22201 aggaactttt aaacatacaa tacccatttc acttaatttt tcaataagtt cttctcgact
    22261 tattttaagc atattcgttc ttatatagat actaggcttt tcactattcg cttccaataa
    22321 atcttcagca aattcttgtc caaatttatc tatccagttt tttataatcc aagagttata
    22381 ggaatacttt atagataggt attttacact atcttcctca gttatgtcca ttatagtctc
    22441 tttagctctt atttcatttc taagaatagc atttatgaaa ccagaagatt ttttatcata
    22501 tttcttagat aatttcacag tttcattaac tgctgcataa tcagatatac tatctaaaaa
    22561 caaaatctga tatgttccca ttcttaaaaa gattttaaca taagttgaca ttttttttac
    22621 ctttattttt gaaagtttat tgattatata atctaaataa tatttatttt ctattactcc
    22681 ataaattaat tctgttgcaa gccctctatc aatgtcactt atttctaaat ttttaaaatg
    22741 cttatttatt gcaatatttg aataattgtt atttttttct atatcacata ataccttaaa
    22801 tccaacttct cttgcatcca tataaatttc tcccttttat taaacataaa aaagggttgt
    22861 ttctaaacaa cccactttac ttttagtcat ttcttctttg agctattagt aacaatctaa
    22921 gtaattgaag tattgaaacc aatgctgctg caacatatgt taaagctgct gctgaaagca
    22981 ctcttctact ttgtcttgac tcactctcat ctataatacc taaatttcca agttgaacta
    23041 ttgctctact tgaagcatta aattctacag gcaatgttat tatttggaat aacactgaag
    23101 ccgaaaacaa tagtatacca atttttaaaa atggaccagc cataataaat cctagaaata
    23161 ttaaaaacca agatatactt gaagcaaaat tcactactgg tactaaacta cttcttattt
    23221 gaagaggtgc ataaccttta gcatgttgta tggcatgacc acactcatgt gcagctactg
    23281 caacagaagt tatagatgtt ccataatata catcttctga caaccttaca gcctttcttc
    23341 ttgggtcata atggtcactt agatgtcctc taaccatttc tatctttaca tcatacaaac
    23401 catttgaatc aagtactctt cttgctgttt gctctccagt atatcctcga cgtgtattta
    23461 ctcttaaata cttgtttgtt gttgaactca ctttaaattg tgcatatata gtaagtaata
    23521 tagctggaat taatacaacc attgttgggt caaatcccca aaaaccgcca tatacagggt
    23581 acataaataa cactctccta aaatatattt ttattataca tcctcttaat attcacttaa
    23641 cttaaaaaca gtttaattac aagctaatat attttttcct gttaaaaatt ctatagcctt
    23701 ttcaatgtct atcatatcta cattagtatt tacacaaggt ccattaggtc ttttgttaaa
    23761 tacacctaaa acaggaattt ttttaatatc ttgtattcca gatgtcaaat ctctttcaca
    23821 tgcaacggct acaacagctt taggtttatt gtctattata atttttctag ctagtgttcc
    23881 tcctgtagct acaaatattt ttacattagt tttttccttt agcttaatta attctcctac
    23941 gttgcatctt ccacattctt cacaattatc tatatcatta gtaactttta atttacaact
    24001 attctcttga atacaatgag gtattaaaat tattatatcc tctggattaa aattatattt
    24061 attactgtat atatattcat tatttaactt tatatatatt cttctaatct cactttttga
    24121 cataccaaat gatgatgcaa taaaagatat aataggaaat aatccactaa caatattaaa
    24181 atttattttc attatatttg agttaatatt ctttccttta attactctat aagtaactgc
    24241 tgttgaacat agtattataa gtattagtga tattattata atatttatta ttaaggaaaa
    24301 tatttgagtt aaacttatta taaaaaaatt aacaactcct atcaaaagcc ctaatattaa
    24361 aatcaaaatc ccaacacttg ttaaatactt ctttatatct gtcatacttt tcacctaacc
    24421 caatactaaa ccagtttcta tagtattccc ttttatatat tcaccaacca acattctttt
    24481 tttattcggc atctgaattt cttcaattaa aagtacatta tctatagtac ttacttttat
    24541 tccatctttg cttacatcaa ttattgtacc tgcatctttt gtacttttct catctaacac
    24601 tttagttttc caaactttca ttatgacatc attataggta gtataagcac ttggccatgg
    24661 atttacccct ctaactaaat tgtgtatttc cctagcactt tttgaaaagt ctatgttacc
    24721 taatgattta ttcattattg gggcatatga aaattcttca tgattttgta cttctcttgg
    24781 agcatttcct tcttcaatta atcttaatgt ttcttttaaa gtctctgctc ctatattcat
    24841 cattttatca tgaagttctc ctgctgttat attttcatca aggtttactt ctgtctttag
    24901 tatcatatct ccagtatcta gaccttcatc catatacata gttgtaacac cagtcttttc
    24961 ttcaccattt attattaccc aatttatagg tgccgcacct ctatattttg gaagtaaaga
    25021 aacatgaaca tttatacatc caaacttagg aatctctaat attccttttg gaagtatctg
    25081 tccaaaagct acaactacta ttacatctgg atttaaagat ttaattgtat ctataaattc
    25141 tttatctcta gcctttactg gttgatatac aggtatatta ttttcaattg ccagttcttt
    25201 tactggactc atacccagtt ttttacctct tccttttggt ttatctggtt gagttacaac
    25261 acccaatatt tcatacttct catctattat tttttgtaaa catggaactg ctatatcagg
    25321 agttcccata aatactattt tcattaaatc aactcctact acttttctat tttgtcaaca
    25381 aataaaattc catctaagtg gtctagttca tgacaaaatg ctctagctaa aagttcttct
    25441 ccctctaact ctattgtttc accatttcta tttaaagctc taacctttac ataatttggt
    25501 cttctaacat ttcccgcttc tccaacaaca cttaagcatc cttcctcatc tatttgctcc
    25561 ccagatgttt ctattatttc aggatttata agttctataa gctcttctcc aatatctatt
    25621 actactactc tttttaagat tccaacttgt ggtgcagcta atccaacgcc atctgcatca
    25681 tacatagttt ctgccatatc atctaataat tgtattatct tttcatcaat tttttcaacc
    25741 ttttttgatt tttttcttaa tactggttct cctatttgaa ctatttgtct taaagccatt
    25801 ataattcctc ctaaatttat aatacactgt ttggattaat atctatagat acactgatat
    25861 tcttatcaaa tatcaaatct cgttttgtta tacatatata ttttattata ccctttaaga
    25921 gattaatttc aatattttcg tctttaaaga gtatttgcca tctataattt tgatttattt
    25981 ttgatattga acatgggttt ggacctaata taaatcctaa atcatttata cccctttttt
    26041 ctaataaata aattaaagat gcatgcatat tttttatatt ttttactacc aatcgctcat
    26101 cttcaccact aacaactaca cttaacatgt tattaaaagg agaatatcca aatgcttttc
    26161 ttatttttat ctcatcttca taaaaacctt cataatcata ttcaatagca tgcttaatag
    26221 catagtgttc agtatcataa gtttgaagta ctacttttcc ttctttatca gctcttccag
    26281 ctcttccaga aacttgagtt atcaattgaa atgtagtttc aaaactttta aaatctggaa
    26341 aatttaatat catgtctgca gacaaaatcc ctactaaagt tacattctca aaatccaacc
    26401 ctttgcttag catctgtgtt ccaattagta catctgcttt tttatcttta aatttattta
    26461 gtatatcatc taaagcacct ttttttgaag ttgtatcttt atccattctc aaagttttta
    26521 tatctggaaa aatatatttt aattcctcct cgattttttg agttccaact ccaaatggct
    26581 taacataagt acttttacat tcaggacact cttttggaat ttctttctca tatccacaat
    26641 agtggcatct tccagtattg cttttcttat gataagttaa agatatatca caattttcac
    26701 attgaaacac atatccacac tttctacaag atacaaaatt tgcataacct cttctattta
    26761 aaaacagtat tacttgatta ttttcttcaa tcgcacaacg tatttctttt tgtaatttca
    26821 aactaaatat gcttctattt cctttatcta actcctcttt catgtctact acttcaatat
    26881 ttggaagagg tttgtcattt gctctactct ttatatttat aagtttgtat tctccgtatt
    26941 ttgccctata atactcttca attgatggtg ttgctgaacc taaaataaga gttatattat
    27001 tttttaatgc cataaatcta gcaacttcta tagcattaaa ttttggattc ttctcagact
    27061 tatatgatga ctcatgacat tcatcaatta ttattaagcc taaagaatta aaaggtgcaa
    27121 ataaagccga cctagctcca attaacactc ttactttacc tgccttgaca gccttataaa
    27181 catcatgttt ttctccttca gatagtttgc tgtgaaacac accaactaaa tctccaaacc
    27241 tacttttaag cctatctatt gtttgaggag ttaatgctat ctcaggtact aagaatatac
    27301 tgtctaagcc ttgatttaga gcaaattcta ttatttccat gtaaacttca gtttttccac
    27361 ttccagttac tccatgaatt aagtatggtt ttttatcatc taaaaacata ttcgacttta
    27421 tttcatcaac tgctttttgt tgctcattgt ttaattttat actttttaac acggatttgt
    27481 aaatactttt tggctctcta taataatcct taaattctaa ggttattagt tttttcttgc
    27541 ttagagaatt tatactttgt tttgaagcat ttaataaatc caataaatca tttatctcta
    27601 cattttcatt atttttaaga aaacttaata tctctttttg tttacttcct acatttattt
    27661 tattttgctc tatataatca tcaatcttat cactttccaa actcaaagat atataacata
    27721 ctttcttttc atttttgtga tttttatatt cccaacataa ttttataatt ccatttttat
    27781 tcattgtgta caaagaacta tttaagtttg gtatatattt taacttatca accttaattt
    27841 tacctttact ctcaatcact ttttctacta tttctctatt tttatcactt aaagttgata
    27901 ttttttcctt caattcaata tcatttaatc cacttaaact ttcacctaat aggactactt
    27961 tataattatt tagtttgtat ccctttggat atatcaaatt tatacattct atataagtgc
    28021 acaaatatct atttttcatc caataaacca actctaaatc ttcttttcta aatattggat
    28081 tttcatctaa tatatcaata atttctttta tttttatttt ttcatctaag ctatctgtaa
    28141 attgaaatac aaaggcttct gttggtttat taccttttcc aaatggtact aatattctgt
    28201 gtccgattct tatgacctca gataaaaata caggaatttg gtatgtaaat aaattatctg
    28261 tgtatatagt attacttcta actattacct ttgcatattt tttcattata aaacctctac
    28321 ttaaatttaa tatactgttc atattttaat atactttaca agacattaaa atttcataat
    28381 ttataaacaa ttccaagtta ttaatattaa ctttatttta ataattaact attaattata
    28441 ttttatactt tatgacaaaa taaatgctga aaactacaat ttaactgaca tttgcgagtt
    28501 tttactcgct aaaattaaat tgcagctttc agccacttta gctgtgataa aatacacaaa
    28561 tatcatattt aaaaaaggtc ttatttctca agcaacgctt taactttatc taaaataaca
    28621 ttggctactt cttctttttt cattttagga tactcagtaa tatttccttc tacatctatt
    28681 atttttacaa tattagtatc agctctaaat ccagcacctt ctttagttaa atcatttgcc
    28741 acaataaaat ctaggttttt cttttttatt tttcctttgg catgttctat caggtcatta
    28801 gtttcagctg caaatcccac tagtatctta tcttctttta tttttcctat ttcataagca
    28861 atatcataat ttctacttaa ctctattgat aaatcatcat tcttcttttt aattttttta
    28921 tcagaataag tttttggctt ataatctgca actgctgcac tttgaatcac tattttattt
    28981 tcacttaaat ttgatactac tgcttcgtac aaatcttgtg ctgactctat ttgaacaaat
    29041 tttttaagat tttgtgggat agaaagattt gtaggacctg atattagtgt tacatcagca
    29101 cctctagata tagcttctct tgctatagaa tatcccattt taccagaaga cctattagtt
    29161 aaatatctta cagggtctat actctctaca gttggaccag ctgtaactat catactttga
    29221 ccttttaaat cttgttcttt agtaagatta gctaaaactt cctccactat tatatctgga
    29281 ttggccagtt tacctgctcc aatatctcca catgcaagtc ttccgctttc tggttcaata
    29341 aatttatatc caaagctctt taaagtttgt atattttttt gaacaattgg attttcatac
    29401 atattagtat tcatagctgg tgctataaga acctgtgctt ttgttgccat aacagtagtt
    29461 gtcaacatat cgtcagctat accatttgca atcttaccta taacatttgc acttgctggc
    29521 actattaaaa ataaatctgc tttttttgct aatgatatat gttcaacttc ccaagttttt
    29581 ggttcttcaa acatatcttc tactacataa ttttgactca gtgattgaaa tgtaagtggg
    29641 ttaacaaatt ctgttgctga ttttgtcatt ataacatgga tatttgcatt tagttttttt
    29701 aacttactta caacatcaca agccttatat gcagctattc cgccgcttat gccaattaca
    29761 acagttttat cttttagcat gaccttactc ctcttctgaa atttgttggt gttgctcagc
    29821 atggtgtcta gcttcttcta tctctatttc ctcttctgtc aataatctat atgttatttt
    29881 tccacttgca acttctttag ttgctacaca tacaggcttc tccttagctt tatttgaaac
    29941 atatggctct tctccatcaa ttaattttct tgctctcttt gaaacagtcc ctactaaata
    30001 gtatctgttg tctatttttt ctagtacctc attgatagat ggttttaaca taattataat
    30061 tcctccttga atttctctat tataatattt ttatatctgc taactttatt cttttcagat
    30121 gatattatac cttctatttc tttagcagat gtttttacat cttcattaaa tataaaataa
    30181 tcataatcct taatcatttt aatttcttca tatgcacagc taaatctctt ctctatttcg
    30241 gcttctgttt ctgtacccct tccaacaatt ctatttttta gttcttcaag tgatggaggt
    30301 agtacaaata taaatactcc ttctggacaa acttctttta tttgttttgc tccttgcatt
    30361 tcaatctcca gtaatacatc ttgtcctttt tctaaacatt ccattattgc tgattttgga
    30421 gttccataaa aattatcata aatttgagca tactccaaaa actcaccttt ttctatcatc
    30481 tcttcaaact tttccttact tatgaagaaa taatttactc catcaacttc tccagttcta
    30541 ggttttcttg tagtagctga tacagaaagt tttattgtat cattttcttt aagtagttct
    30601 ttgcaaatag taccttttcc tgcacctgat ggtcctgata caactagcaa taatcctttt
    30661 ttcacaaaca tttaagcatt ccccttctaa tattgtattc ttactctata ttttgtattt
    30721 gttcccttat tttttctaat tcgcttttta cttctactac taaattagtt atatttatat
    30781 cagacgactt cgaacctata gtgttagtct ctctattcat ttcttgtatt aagaaatcta
    30841 tttttctacc tatagagtcg tctttaaata tagtattttt aagttgccct atatgacttt
    30901 taaatctaac tatttcttct gttatactgc ttttatctgc atatattgca acttcttgcg
    30961 ctaatctatt ctcatctatt atacttgggt catccagtaa ttcacttatt ctcaaattca
    31021 atttttctct ataatcttcc acaacactac ttgaatattt ttcaatttct tctatatgat
    31081 tttttaataa atcacatctc atggcaatat cttcagctaa ctttttacct tcttcacttc
    31141 tcatttctct taatttaatt aatgcatctt caactgcttg attcaacatt gaccataata
    31201 aatcttcatc ttcttccttt tcttctattt tcacaatatc aggaaacttt gcaacattca
    31261 taacactaat atcatctata atgtcaaatt tgttttttat ttcttttagt atgtctatat
    31321 actgagatgc taatccctca tcaaaattta agtttacatc ttctttgcct aataagtcaa
    31381 actttatgta taaatctact cttcctcttt ttacataatt tttcactaaa tttctaactt
    31441 tatcctctaa aaatgaaatt tttcttggaa gacgtatgtt tatatctgaa tacttatgat
    31501 ttatcgtttt acactctact aaaaagtaat aattatcatc cttgtattct cctcttccaa
    31561 aaccagtcat acttatagcc attaattaaa cctccaaaat accttcacat atttttactg
    31621 ctggacctgt catataagag aaatctccta catctatttt aactgttcca ccttgagacg
    31681 ttacatttac actactttct accatattaa gtaaattgca cactattgct gatgcagtca
    31741 taccagtacc acaacctaga gtatacccac aacctctttc ccaagtataa acatgtatat
    31801 tatacttatc ttctaccttt acaaaattta cattcgtttt ttcaggaaat attttattat
    31861 tttcaatctc ttttccatat ttacaaacat cctctacatc taactcatct actataacta
    31921 ctgtatgagg aacccccatc aacatagaag taactttaaa ctcactatct aaaaccttta
    31981 tactttcttg cacaaaacgt tctttatttg taagtacagg aacatcctta gctataaaac
    32041 ttccttttcc catattgact cttatagagt ttatttctct attggtaagg tttattttta
    32101 tatcttttat accatctaat gtatctactg taaaactttc ttttttgact atacaattat
    32161 cataaacaaa tttacaaaaa catctaagac catttccaca catagctgct cttgaaccat
    32221 cagaattata atataccatt tctacatctg caacttttga atctttcaca actaacaatc
    32281 catccgctcc tacagaaaat cttctatggc atactttttt tgctaaagca gtataatcag
    32341 atgggtttat ctggtcgaat cttccatcta ttgctataaa atcatttcct attccatgta
    32401 gtttccaaaa tttcataagt atcgctcctt cgtaaattaa cacttaaact attataacat
    32461 aaaaaaattc ccataaactt ataatttatg ggaaaaattt tgtaatgaca actataattt
    32521 gaggatgtga gggagagttg ccattacaat tagggtaatt atattatgtt tttaagctag
    32581 ttctttctat gtttatataa taatatttct atatgaattt tatgtatgtt ttatttaaac
    32641 tgtctatgaa ttttttaatt aaacatctga taaccagcta taattctttt ggtagatatc
    32701 tattaacata cacctattaa ttctttaagt gtttttaaaa catagtctaa atcttcctta
    32761 gtaatatcta catgagttac aaatctatat tcaccatttt ccattttatt aatttttata
    32821 tttctttcaa aaaattcttt tattaaaaca tcctctttta taatatcttc actcatctta
    32881 aaaaatacca tacttatatc tggattaaca ttgttcactt caatgccatc aattttattt
    32941 agttcacaag ctaaatattt tgcattttca tggtcttcat tcaatcttaa agtcattttt
    33001 tcaatggcaa ttataccagc tgctgcaact atacctactt gtctcattcc tccacccata
    33061 actttttgct ttcttctagc ttttgctata aagtctttac ttccagcaag tatagaaccc
    33121 attggtgcag ctaaaccttt tgacacacag aacataacac tatcacaaca ctttgctatt
    33181 tctttagctt ctacacccaa agttaatgct gcattaaata atcttgctcc atctagatgt
    33241 actggcaagt tatgtttctc agctattttt ttaatttctc ttaaattatc aagacccaca
    33301 acagttccac ctgaatatgc attttctaca catataagcc ctgtctgtgg ttcatgtatg
    33361 tcatctcttc tgattgcctt ttcaacaagt tcaggattaa gagctccatt ttcgtcttca
    33421 tctatagttc taagctgaac tccagctatt acagcaggtg ccccaacctc atgagttaca
    33481 atgtgatatc ctttaccaat aattatttct tgtcctctta gacaatgtgt gaatagagct
    33541 aattgattac caaaagttcc acttggaaga aaaatagcat cctcttttcc aactttagct
    33601 gctgctaatt cttcaagttt atttacagta gggtcatctc taaagattga atctccaaca
    33661 ggagcctttg ccatagcttc tctcatttca tcagtaggca tagtaacagt atcacttctt
    33721 aaatcgataa atctcatttt ttcaaccttc tttcatattt atatactata gaaccttaaa
    33781 attgattttt tctgtaaagt tcatatactt tcatagctta tactttacac aataattata
    33841 ctatatttac tttatattta atctacttta aattattaat tttaaaaata aagtttcctt
    33901 tctaataagt ataaataatt atacaattta aatattaatc tctattgttt aaattccctc
    33961 tagtcacaaa gcgtatatta tgtgtataat taatttactg actcattttg gtcaaagaaa
    34021 ggggtgctat acatggcatt agatggatta gttatacatt caatagtcga tgaattatca
    34081 tcaaaactta caggtggaaa aatagataag atacatcagc ctgaagatga tgaggtaatt
    34141 tttaatataa gaaataataa agaaaatttt agattggttt tgagtgcaag tgcctcaaat
    34201 cctagagttt atcttacaag caattatcaa aaagaaaacc ctctaaaagc acctatgttt
    34261 tgtatgttac ttagaaaata catacaaggt ggaaatatag tagaaatttc tcaaataggt
    34321 tttgaaagaa taataaaaat aagtgttgaa tctttagatg aactaaagga aaagacaatt
    34381 aaaaatatta tgattgaaat tatgggaaga catagcaaca taataataac tcatggtgaa
    34441 gaaaataaaa ttatagattc tataaaaaga gtccctttta gtataagtag agttcgtcaa
    34501 gttttacctg gtcatgatta ttcattacct ccagagcaaa acaaactaaa ccctcttgat
    34561 gctatatcta aggatttgtt cattaaaaac ctagaggaat cagaagaatc tatctttaag
    34621 tctatatatt ctaaatttct aggtataagc cctattatag ctaaagaaat ttgctacaga
    34681 gcaggtataa atcaaaacac catcataaaa gatatatctg atgagcaatt tgattcttta
    34741 cacaaagtat tttgtaattt atttaatgat ataaattcta ataaatactc accttgcatt
    34801 gttatagata aaaaggtgga tagggtagtt gatttcagct gtataaacct tacattattt
    34861 agtgatttat catatataaa taaggatagt atgtctcgta tacttgaaga tttctataga
    34921 actaaagata ttaaagatag aattaatcaa cgttcatctg atttaaaaaa gagcatatct
    34981 gttaaacttg atagacttta taataaactt aaaaaacaag aagaagaatt atcagaatct
    35041 gaaaatgcag atatttataa gataaaagga gaattgatta cttcatacat ctatatggta
    35101 gaaaaaggta tggaaagtat tgaagttgct aacttctatg atgaaaattg taataatgta
    35161 acaatagaat taaataaaaa cttgacacca tctgaaaatg ctcaaaaata ctttaaaaag
    35221 tataataaaa tgaaacatgc taaagtcgag atatctcatc aaatttcctt aaataaggaa
    35281 gaaatagatt acttagaaaa tataatttta agtattgaaa attgtgaaaa tttagctgaa
    35341 ttacaagata taaaagagga actcgctaaa gttggatata tcaagactca aaagaaaaac
    35401 agtaaaaagg atactattcc atctacaaag cctcatgaat ttatgtcttc tgatggattt
    35461 aaaatattgg ttggtaaaaa caacaagcaa aatgactatc ttacactaag acttgctgat
    35521 aacgacgact tatggatgca tacaaagaat attcctggtt ctcatgtaat aataaagtgt
    35581 gctggtaaag aagtccctga taatactgta tttgaaggag ccatgctagc cgcattcttt
    35641 agtaagtcaa aactttcttc tcaggtgcct gtagattata ctaaaagaaa aaatgtaaaa
    35701 aaacctagtg gttctaaacc aggtatggtt atttatgaga ctaacagtac tatttatgtt
    35761 actcctgaag aagaaacagt agctaaactt aaggttaaat ctgaataaat tctgcactaa
    35821 atataaaata agtgttagac aagcacacat tatcatttaa aatgaaaata gtggtcttat
    35881 tatgaagtac aataataagg ccactttcta atcaacacac ttttatattc taataactat
    35941 acttatattc ctaataagta tttttagtct tcttatatag atataattaa aaagttaata
    36001 ctaaaagcta ttttttaatt tattgatttg ttttagtagt tcattgtatt ctgattcata
    36061 aagttctttt tttagaccat ccttttctat agataacata gatacaatag ttataagtct
    36121 attttctaat actagaagat tttctttttt aatcttactt tctctactag gatttttttt
    36181 gtttctatta attatatatt catcataatt acaatcaaac tcccttattt tagtgtcttt
    36241 tatctctata atatagttgc aaatatgtga tacaaatact ctatcatgag atactataag
    36301 catagtttta tcagtattta tcagtgcctt ttctagtgct tccatagatt ttatatctaa
    36361 gtaatttgtt ggttcatcaa atattaaaaa attattatct tccaatatta ttttacatag
    36421 tgctattttt accttttctc caccactaag tacttttact tttttgtaga catcatctcc
    36481 tttaaatcca aacaggttta agtttgtcct tataaaagat tcatcaaatg aactattaac
    36541 tttagtattg tataaaacac tctttgcctc atctaataag ctttgatgtt ggtcaaaata
    36601 acctaccttt actttgttat ttatttttat attgtcattt ttattattca atatttcttt
    36661 tatcatagta ctttttccac acccattatc acctaataat gctatctttt tacctctttt
    36721 tatcttaaaa gaaacattat ctaatagcag tttatttcct aattttagag tcatatcctt
    36781 tatctctact agatttttgc taattatctc cataccattt tgaatcttaa tcttcatttc
    36841 tatagaagct ttaggtttat tttttacctc aagtttatca attctacttt ttatagcctt
    36901 cacatttgag tccaactttt tcttattagt ctgacccccc attttgtgca atctagcttc
    36961 tgaattaccc attctttttg gtgtttttct tatgccatca cttaaagatt ttttatgtaa
    37021 aagagctttt tcaagccttt ttttctcttt aacatattcc tgatactcaa aataagctct
    37081 ttttacttct tcatcttttt gcattaaata atcagtgtaa ttgcctttgt aggcttttag
    37141 ttttccatct tctagttcta atatagttgt acacaactca tccagaaaac gtctatcatg
    37201 agatattaat aataatgctc cctcatgcct tttaaacatc tcttccaaaa ctccaatact
    37261 ttttttatct aagtttgaag tcggctcatc agctattatg atattttttt tattgctcag
    37321 tgcatctgct atttttaact taactttttc ccctcctgat aagtactctt caaatctatc
    37381 tggagcattt aaaagactct tctctttact atcactacat atttcaatat tattttcact
    37441 ctgacttata taggaataac tttctgtaag gtaaatatat ccttcatcaa tttctatttc
    37501 acccaatatt gcttttaata atgttgtttt tccttgacca tttgaaccaa ctagccctat
    37561 tttgtcgttt tctaatattt caagtttatc tatatctaaa attaatttgt ctgcataata
    37621 tttttttaag ttctctactt ttactaataa cataaaaaat ccctccttaa cactcttaaa
    37681 tataaggaga gattctataa ataggcataa caaataaacg tatagactat aatctactct
    37741 ccttaattta agcatcgcaa ataaagccac caaaataggc ttttcaaaaa ttacaatact
    37801 ttaattataa aaaatagatt atattctcat tgtcccagtt accttgccct ttctttcatt
    37861 tatttttaat cattatatca acattaatta caaatgtcta tagaaaatta actaatttaa
    37921 attattcaat atacaattta ttattcttca actttaaaat cctgattatt taaaattctg
    37981 tttaatacaa ttccaacaat tgcagctaga ctaaggccag ttatctttac tgtacttgtt
    38041 atagggattc caatagaaat acctttatta gacaaatatg ttgttccaag acctattaat
    38101 aatataaatg ctattatcaa tatatttatc ttactttcta cgcaagaact atctcttata
    38161 gtttttaatc ctacataagt tatcattgaa aatagcatta tactaattcc acccataact
    38221 gaacctggta tactttgaag aaatccacca aactttccta cacatgcaag caatatggca
    38281 aatattgctg tacgtctaag tattgaaggg tcataatttt tagttatggc aagtactgct
    38341 gtattttcac cataagttgt attggctgga cctcctaaac aacctgcaac tatagttgca
    38401 aatccatccc caagtaaagt tctatttaat ccgggatttt ctataaaatt tttacctact
    38461 acagcaccat tagtagtcat atcccctata tgttccataa atacagctaa cacaactgga
    38521 gatataatca caattgctcc taaactaaac ttaggaactg taaagttagg tattgcaaat
    38581 aaacttgcct ctgtaattgt agttatatct actaatccta atatcaaaca gattatatat
    38641 ccagaaaaaa cggctattaa tattcctaat tgttttataa atcctctacc aaacttattg
    38701 gttaaaattg ctattgctaa agttatgaat gctattacaa aattggctga agccatatta
    38761 aaagctgttg gcaataaatt taatcctata actactatca tagctcctgt aacttgtgca
    38821 ggaaaatata attttatttt atttactcca actattttta ctataatcga cattataata
    38881 taaattaatc ctgcaactat tataccacct tgagcatacg ctaaatctcc accatacgcc
    38941 tctttagctg ctaatataac tggtataaac gcaaaacttg aacctaaaaa ggcaggtact
    39001 tttccttctg tacaaaaatg aaatatcaat gtaccaacac cagcacagaa aattgcaact
    39061 gacggattaa aacctgtcaa aatgggaact aatactgtcg ctccaaacat ggctaataga
    39121 tgttgtaaag acattatcgt ttttttcata aaaacctcca aaattattat atataaataa
    39181 atcctctgtt taattaatat cttttaatta attatcattc cctagattct attttttaac
    39241 ctctatatac ttattatata atttattatt attttacatt ttacgacatc aaaactcaaa
    39301 taaaggagaa taaagactac attaagaccc acatccataa aattataata tttattttgt
    39361 gcaatctatg taagctttat cctccttcct gactattcaa tgctaagctt ttatataata
    39421 cttaaaaatt attattcttt gattgtaact gaatcttctc catcaatttc taataaacta
    39481 acagatataa tttcatgtct agaagtaggt acattttttc ctacataatc tgctcttata
    39541 ggcaactctc tatgccctct gtccactagc actgctaatt gtattgattt aggtcttcct
    39601 atatctataa tagcatctag tgaagctcta acagttcttc cagtatataa aacatcatca
    39661 actaaaacta ctgtcttatc atttacatta aaatctaaat aagttccttt aactacaggt
    39721 tcgacatcaa ttttttccaa atcatctcta tacaaagtca catcaatatc tcctgtgtct
    39781 acttttgttc cttctatctg ttctattttc ttagaaattc tatttgcaat tggtacacct
    39841 ctagttttta ttccaactaa aactagattt tctacacctt tatttctttc aattatttca
    39901 tgacttattc tagtaattgc tcttgctatt gccttttcat ccattaattg agctttttct
    39961 accatatcat aattattgtc aaagatataa tatatctatt tgacaaatct actcctttct
    40021 tttaaatttt tataaattta tttttactta aaatttcaat ttaaacaaca aaaaacttct
    40081 catcacaaga caagaagctc ttatataaaa aatatacagt gtaatttagc aattgtctta
    40141 cgatatctct gtatcgttta aagataattt tttaatctat caattcaaat tatatattta
    40201 tatttcaaat ttttcaagct tttttaaaca aaatacttta atataagata ttttatttaa
    40261 atttcactaa atttatttac ttacataaaa acaaaaaatg atttataagt attatacaaa
    40321 tgattatttt atctttttaa taacttcctt aaaatactca ggtaattctg aattaaattc
    40381 gatatattcc ttagtagtag gatgtataaa tcccaacttc ttagcatgca aagtttgtcc
    40441 ttttaattta aatttagttt cttttggacc atatacataa tcacctaaaa gtgggtgatt
    40501 tatagacaaa gcatgaactc ttatttgatg agttctacca gtttcaagct tagctctcat
    40561 atgtgtaaat ttatcaaatc tttctacaac ttcaaaatga gtgattgcat tttttccatc
    40621 ttttactaca gccattttta atctatcctt aggatttctc cctataggtt tatccacagt
    40681 tattttattt tctttaacca caccatagca tataaattca tactctctag ttatagtatg
    40741 ttcttttaat tgttctgcta gaaaattatg tgcataatta ttcttagcta tcataagaag
    40801 accagaagta tctttgtcta ttctatgaac tataccaggt ctaataactc catttattga
    40861 tgacaactta tctttacaat gatataaaat cccatttacc agcgtatttt catagtttcc
    40921 aggagcagga tgtacaacca tatcctgagg tttatttatt attaaaacat catcatcttc
    40981 atatacaatg tctattggaa tatcttgagg tacaacttct aatactttag gttctggtat
    41041 ctctataaca atattatctt cctctttaac tagatattta gccttttcta ttttattatt
    41101 aacctttact tttccatctt ttataatttt ttgtatataa cttcttgaca tatcacctaa
    41161 ttgttctgct agataaatat caagtctaac ttcttcttcc tcttcaagta ctaaaaactc
    41221 ttttacttca tacattaaat cacctacttt tactttcaaa aaataaaaca tatatacata
    41281 aaaacacagt tcctacaact acaaatacat cagctacatt gaatacatat tcccatataa
    41341 ttctaaagtc gaagtaatct actacaaaac ctaatcgtac tctatcaatt agatttccca
    41401 atgcaccaga tataattaat ataattccca attttccaga tatatgtact tttcttgtat
    41461 taagatagta taacccaaat actgttgcaa gtaatgcaac aattataaat atccattgat
    41521 tattttgtaa taatccaaat gctgcacctc tattttccac ataagttaaa tgaaatacat
    41581 tatttattat tggtattgtt gatacatcta ccaaattatt caatacccac attttagaca
    41641 gttggtctaa gcctatgagt agaattatta ttaatatata tagcaatgat ttttccccct
    41701 ataaattatt taaaaaacaa ttttatttga aacacactat tattattata caaaaaaaat
    41761 gctctaaaga gcaactatct taaaatatta atgatgctta taaatttaaa atacaagcat
    41821 cattaatatt ttaataatta ttttttattt ttgtacttgt ttatttttat ctaaatgttg
    41881 tataagctct gatattgtta caaacttata ccctttttct tttagcttag gtattactac
    41941 ttctaaagct tgtatagttt gtgacttatc atttcttcta ttattataat catgtaaaag
    42001 tattatatta ccattttgaa cttttgtaag aatagtattt ataatattgc ctactccagg
    42061 attagaccaa tctctagggt ctaggtttga ccagagaatt atattcatct ttttagaaac
    42121 tataatatca caaacactct tactcattgc tctatatgga ggtctaaata tttttggttc
    42181 tactccgatt acattcttta cagcattttg tgtatctgtt atttcttttt ctaccttatc
    42241 atagctattc tttgcaacat ttatatgtgt aaatgtatga tttcctatct catgtccttc
    42301 agtatactgt ctttttaata attctttgtt atattctact ttttctccaa ctacaaaaaa
    42361 agttgctttg acattattct tttttagtat atcaagtact tgtggtgtga atacctcatc
    42421 tggaccatca tcaaacgtta gagctatgac cttttcatca tcacttccct ttttaattat
    42481 tacatcttca taatcactaa ttttattttc actcaaacta taagcttctt ttaagtcatc
    42541 ttgattatgt gtattatgat tatatgtata tacacctact atagataata aacatataca
    42601 tataccccat atagatttct tcatgtcatt acctcttttc taattcattc taacaatata
    42661 tgattcagtt ttctaatctc ttaatattcc attcttattt aaatcagtta aatttgttag
    42721 gtcttctgaa taaaaattag aatcttctac atcaggattt tgcattgcat cacctaataa
    42781 attattttct ttctttgcac aatcattaca taaacttgta tctggcaata tatccaatct
    42841 ttctaaatct atttgattat gacataagtc acaagtccca tatgttccat tctctatttt
    42901 agataatgct aaatctattt gatataatct tccctcttca tgatttatta gactgttttg
    42961 catattttgc atatataaat ctgttcccat gtctcctata tgattatcat aactagataa
    43021 ttctcctgat gaatactttt cgcttgtatg tttagtcgta tctccaaata aagtattatc
    43081 tttcatatcc tctactaaat tagttaaact ttctctttct ttcaataatt tatctttata
    43141 ctttttagta tccattttta acacctctaa ttttaatatt tatatcataa aaatagtatg
    43201 tggaaaagta aattaaacat acaataaaaa taagatttga ttcaaaaaat ggaatgtctc
    43261 aaaataaatt ttttagttca ttaaatcctc ttttttgtat aaagtcaaat atatttttta
    43321 taatttcaca atgaaaaaga gacttatctc tatcttgaga cagcctcttc tttgaacata
    43381 gtttaaatat aaatttttaa tttttatagt tattatttta ctaacttgcc aatttatgat
    43441 tattttgttc cttaatatgt ttcgcttttt taaagaataa aagaccaaga attattgcta
    43501 caataacacc tattccatta gatatattaa ctggaagtct aaatccttct tttgcttgca
    43561 ttatataact aaatgttact acagtcataa aaagtgctgg taatgtagtt atataatggt
    43621 ttttatttgc tttaactaaa tatgcagaac cagtccacaa cattatcatt gcaagtgttt
    43681 gatttgccca agagaaatat ctccaaatta tattaaaatc tataaatgta agagcaacgc
    43741 caaccgcgaa caatggtaaa gctattaaaa atctattttt tactgcatct tgattataat
    43801 tcattgcatc tgctattgtt agcctagcac ttctaaatgc tgtatcccca gaagttatag
    43861 gacatgccac aactccaagt atagctaaag ctcctccaat aggacccatt aatccaacag
    43921 atactgtgtt tactactgtt gctgcaccac catcattaaa tattacatta agttgaggta
    43981 taccatggaa atatgacatg gctgctgctg cccaaactaa agctattata ccttctgcaa
    44041 ccattgaccc ataaaatact ggtcttgcct cactctcttt ttcaacacat ctcgccataa
    44101 gtggtgattg tgttgcatga aaacctgata ttgctccaca tgctattgtt ataaataaat
    44161 atgggaataa aggtgttcca tttggattca tattttgtaa atttatattt ggtatgttat
    44221 atccctttat tattaatcct ccagctacac ctactgccat aattaataat gctgctccaa
    44281 ataaaggata tatttttcca atcaatttat ctattggtaa aacagtagcc ataatataat
    44341 aaagtattat tactccaacc catatttgtg cgtttacacc tgtcaatgtc tttaacaatc
    44401 ctgctggacc tgttacaaat acaactccaa ccaatactaa aagaataaca ctaaatattc
    44461 tcattgctat ttttgcacca tttcctagat aaatacctac caactctgat gcagatgtac
    44521 ctttgttctt cattgataaa tatcctgaag caaaatcgtg tactccacct gcaaagattg
    44581 ttccaaatac aatccataaa aatgctgatg gtccaaataa cgccccttgt atagctccaa
    44641 atataggacc taaccctgct atatttagaa actgaattaa aaagattctc ttccatttca
    44701 ttggcacata atctactcca tctctacagg ctatagctgg agtttggttt ttgtcattga
    44761 ttccaaaaac cttttctaca aaagtaccat agataaaata acctactata agtaccacta
    44821 ttgaacctaa aaaacttacc ataaattcat ccccttagtt tttatataat tgctttataa
    44881 atttttatga gtttaagaaa actatttcca gttcaaatag taatactttt ttatgctaat
    44941 ataaatatta attacattaa gtgcatattt aagtacatga aatgacttta tatatgcatt
    45001 aacaacctaa attcttttac tttacttcta ctgacagtta cttcatagtc attatttttt
    45061 aatttcaata tgtatgtatt attaaaccag ggtataattt cttttacttt tgttaagttt
    45121 acgatataag acctatgtgt tctaaaaaac gttttatcat ttataagatt ttcgacttct
    45181 gatatgcctt cttttaatat aaacttttct ttctcagtat atatgaatgt ctgcctttca
    45241 ttagcttcac aataatatat atcatcaata tctattacaa ccaatttatc acctttccaa
    45301 aggctaattt tatgtgttat ttcttctata tcctgattta ctgtttcttt tttatatttg
    45361 tataaattac tattaccact agacagttca atatcactcc tctcactttt ttctaattta
    45421 ttcagcatag aaataattcg ctcttcagaa tatggcttca atatataatc aaatacgtcc
    45481 aaagaaaaag catctactgc ataattttca tatgctgtta taaaaacaat tttaggtctt
    45541 gatttaaact gatttaatgt ttttgcaagt agcattccat caagatgagg tatatttata
    45601 tctaaaaaaa ttacatctat tttattttcc tgaataaagt ctaatacctc tataccattg
    45661 gtaaattcac ttactacctc gatactactt ttattttcaa taaaatatct caattccttt
    45721 cttgctggaa actcgtcttc cactattata gctctcatac ttctactcct ttacataaaa
    45781 tactatcaaa gtcccattat ctattcttct tatattaagc cctcttccat acatcagttt
    45841 aagccttaaa tgtacattat aaagcccaat tttattttct ggcatattgt cttgatatac
    45901 attatttata atttcctcac ttatgcctat accattatcc tctattgata ttctcacagt
    45961 attttctaat gaatatggca acttttttat ggatattttc actgcaccag ctctaccact
    46021 ttctaaaata ccatgtatta ttgcattttc cactagtggc tgtataatta aacttggaat
    46081 ctttatattt acatcttcta tatcataaga tactgttagc aactctccaa atctagcctt
    46141 ttctatctct acaaaagatt taacctgttc aatctcttta tttatatcaa ttaaattatc
    46201 actatattca agattatatc ttagataagt tgataaattt attattaact ctctagcctt
    46261 atctggacta aatcttataa atgaagctat tgtgttcagg gaattaaaca aaaaatgtgg
    46321 attaatttgt ctttgaagtg cttttatttc tgctttattt ctcgcttcta tatccttttt
    46381 atctttctca atcccctcta ctatagatat aacaaatcct attccaatct gcccaactat
    46441 taaaggtaaa tatattccac caattatttg aattgctaaa gaatgtggat atgatattaa
    46501 gtatataagt agtatagtaa aactttcaga aatcatgcct accaatatac catacattac
    46561 tctatgttgc tttggtatcc tcttatatag aaatcctgat aatacacctg ctagtatact
    46621 tgataaaaga cacggaacag atgttatccc tccaatatcc ataaaatatc tatgaatccc
    46681 cgaaaaaact cctgctgtaa tacctaccat tggaccaaat aaaatgcctc ctgatactat
    46741 agatattatt ctagtgttta ctattgaccc catataattt ataccactgt aagttccaaa
    46801 tatggctaaa aaagtaaaaa ctaatgctat acagaccaaa tcattaaagc tatattcttc
    46861 tttttgaaat atctgcttaa atatttttaa ctttgttatt agaagaaata gtactacaat
    46921 gaggcttgtc ttttctatta atgtgattaa aattgatgct ttcatatttc ccctttaaaa
    46981 aataaatctt tacacactct aaatttatta tgtttgttgt aaattgtata tttgaagtaa
    47041 tcctatctcg acattttcca catttaacct tttaattaaa taaaaaaatg gatatacttc
    47101 tattaacaac taatactaac tgactaacac aagcttgtaa taacttgtag ttatcattaa
    47161 ctttacaatt ctagtattta attttatact ttagaaatct cttttcttag aagtaatact
    47221 ttcgaatatt atataataca atgttatgta gattattact catacaacat atttctttaa
    47281 aaattttata ctattctttg ttcaaatatc tcatttgatt taattgtgct aaacacacat
    47341 tatcatctct gtgttatata tttattatat agccgtatat ggataaagta aataaatcct
    47401 atatttaaaa taatatgttt aacaataaat cattataatg cttataatgt atatttacca
    47461 taataatttg ccaaccttga aagttacttc cataattatt gttaaataat tcttccaaaa
    47521 gggatacccc taaattttat tttctgaatt atatgatcat aactattttt atcacgtata
    47581 ataataaaaa ttccagaaaa tttttattat ctaagttaat ataaaacata ttatttaata
    47641 taagtaaatt ttttataata atatattgat ttaatttact ttattattgt aaactataag
    47701 tataagatat actatataaa caagcataaa tataccttaa ttctaactat ccttaatttt
    47761 atttatctcc tagtgttatt ttacgtattt ttctataaaa tacttttagt tttttacttt
    47821 tgaatttttt ataggagggt atttagtgga tatattaatt tttgataatg atgtttgttt
    47881 tggaataaaa ctaaaagaaa agataaataa tattttaata aaagaaggtt ttgataacga
    47941 tgttgtaaga ttatattata atgcaaattt gttattaaaa gagcttactg aaaaaaataa
    48001 agtaagaata tattttatag ttgtagacgc aaaatataag ataagtaatg aattatgtga
    48061 tggattatgg atagcacaaa aaattagaga atcagattat attagtccaa tcatatttct
    48121 tacaaatcat atagaaatga ttttagggat cttcgattat aggttataag ttatggactt
    48181 tatactaaaa catgatatgg aaattgctga aagtaagata aaagcttgta ttaaaattgc
    48241 tcataaaaga tacattaaag aaaaaaatta tcgctctaat ttttttacta tctactcaga
    48301 ttcctcacta tggaaaattt catttgatga agttatatat tttgaaacaa gcactattcc
    48361 acataaaata aaattagtga ctacttctag aatatttgaa ttctataaaa gtttaaagtc
    48421 tttatcagat ttagatgcat gctttgttcg tgtacataaa tcttttgtag ttaataaata
    48481 tcatattgtt tctatcgacc taaagaaaaa taatgttaaa atgagtaacg gccatatatg
    48541 tcgtatatct aatacatata gaaatatttt aaagaacatt attaaaagat aataaataga
    48601 aaaaggggat gtctcaagat aaaaatttga accatctctt tttttataca aaaaaagtag
    48661 ttcctaagaa tcctctatat gataatcgtt ttgtgaaaaa acacatactg actatatcat
    48721 atacaattgc tattagacat tgtaacaatt gttcctagat tatgattcaa caagatagct
    48781 cattaaatca taaaggtatg aaatagtctc tttaaaacat tcagttgtta ttttgtactg
    48841 acatatcata taaatacata tttttatgat atatagctac atattttatg aaatttatat
    48901 aaaaaattct tatttagatt atataatcta aataaattaa aattcaaaag ttaattaaac
    48961 taatattggg agggagaata aatgaaaaaa tttagaaaac ataaaagtat tagtaattgt
    49021 atatctatat tgttgatatt atatctaact ttaggtagtt tgttacctaa taacatttat
    49081 gcacaagact tacaaagcta tagtgaaaaa gtttgcaata ctacttacaa ggctcctata
    49141 gaaagaccag aagattttct taaagataaa gaaagggcta aagaatggga aagaaaagaa
    49201 gcagaaagaa tagagcaaaa acttgaaaga tctgaaaaag aagcattaga atcatataaa
    49261 aaagattctg tagaaataaa taaatattct cagacaagaa attattttta tgattatcaa
    49321 atagaagcaa attctcgaga aaaagaatat agagaacttc gaaatgctat atcaaaaaat
    49381 aaaatagata aacctatgta tgtctattat tttgaatctc cagaaaaatt tgcatttaat
    49441 aaagtaataa gaacagaaaa tcaaaacgaa atttcattag aaaaatttaa tgagtttaaa
    49501 gaaactatac aaaacaaatt atttaagcaa gatggattta aagaaatttc tttatatgaa
    49561 cctggaaaag gtgatgaaga acctacacca ttacttatgc acttaaaatt acctagaaat
    49621 actggtatgt taccatatac aaatactaac aatgtaagta cattaataga gcaaggatat
    49681 agtataaaaa tagataaaat tgttcgtata gttatagatg ggaaacatta tattaaagca
    49741 gaagcatctg ttgtaagtag tcttgatttt aaggatgatg taagtaaggg ggactcttgg
    49801 ggtaaagcaa attataatga ttggagtaat aaattaacac ctaatgaact tgctgatgta
    49861 aatgattata tgcgtggagg atatactgca attaataatt atttaatatc aaatggtcca
    49921 gtaaataacc ctaacccaga attagattct aaaatcacaa acattgaaaa tgcattaaaa
    49981 cgtgaaccta ttccaactaa tttaactgta tatagaagat ctggtcctca agaatttggt
    50041 ttaaccctta cttcccctga atatgatttt aacaaaccag aaaatataga tgcttttaaa
    50101 tcaaaatggg aaggacaaac actgtcttat ccaaacttta ttagtactag tattggtagt
    50161 gtgaatatga gtgcatttgc taaaagaaaa atagtactac gtataactat acctaaaggt
    50221 tctcctggag cctatctatc agctattcca ggttatgcag gtgaatatga agtactttta
    50281 aatcatggaa gcaaatttaa aatcagtaaa attgattctt acaaagatgg cgctataaca
    50341 aaattaattg ttgatgcaac attgatacct taatattttt tcacataaat aatttaatat
    50401 atttcaaatt aaaggaggag aaataatgaa agtacaaatg aggaataaaa aggtattaag
    50461 ttttttaaca cttacagcca tagttagtca agcattagca tatcctgtat atgctcaaac
    50521 tagtacaagt agccattctg ataataaaaa agaaattata aatgaagaca tactcacaaa
    50581 caacggatta atgggatatt atttcacaga cgaacacttt aaagatttaa aattaatggc
    50641 acccataaaa gatggtaatt taaaatttga agaaaagaaa gtagataaac ttctaaataa
    50701 agacaaatca aatgtaaaat ctatacgatg gacaggaaga ataattcctt ctaaggatgg
    50761 tgaatataca ttatcaactg atagagatga tattttaatg caagtaaata atgagagtac
    50821 tatatcaaat acacttaaag ttaatatgaa aaagggtaaa gaatataaat ttagaataga
    50881 gctacaagat aaaaatttag gttcaataga taatttgtca tcacctaatc tttattggga
    50941 attagatggt attaagaaaa ttataccagc agaaaattta ttcttaagag attattctaa
    51001 tatagaaaaa aatgatccat ttatcccaaa taacaatttc tttgacccaa ggttgatgtc
    51061 tgattgggaa gacgaagatt tggatacaga taatgataat ataccagatt catatgaacg
    51121 aaatggatat actattaagg acttaattgc agttaagtgg gaagatagct ttgcagaaca
    51181 aggctataag aaatatgtat caaattattt agagtcaaat actgctggag atccatatac
    51241 agattatgaa aaagcttcag gttcttttga caaggctata aagaccgaag caagagatcc
    51301 gttagttgca gcgtatccaa ttgttggagt aggtatggaa aaattaatta tatctacaaa
    51361 tgaacatgcc tctactgatc aaggtaaaac tgtttccaga gctactacta acagtaaaac
    51421 tgaatctaat acagctggtg tttctgttaa tgtaggatat caaaatggat tcacagctaa
    51481 tgtaactaca aattattccc atacaacaga taattcaact gccgttcaag atagtaatgg
    51541 agaatcatgg aatactggat taagtataaa caaaggagaa tctgcatata taaatgcaaa
    51601 tgttagatat tacaacacag gtactgcacc tatgtacaaa gtgacaccaa caacaaattt
    51661 agtgttagat ggagatacat tatcaactat caaagcacaa gaaaatcaaa ttggcaataa
    51721 tctatctcct ggagatactt atcccaaaaa agggctttca cctctggctc ttaacacaat
    51781 ggatcaattt agctctagac tgattcctat aaattatgat caattaaaaa aattagatgc
    51841 tggaaagcaa attaaattag aaacaacaca agtaagtgga aattttggta caaaaaatag
    51901 ttctggacaa atagtaacag aaggaaatag ttggtcagac tatataagtc aaattgacag
    51961 tatttctgca tctattatat tagatacaga gaatgaatct tacgaaagaa gagttactgc
    52021 taaaaattta cagaatccag aagataaaac acctgaactt acaattggag aagcaattga
    52081 aaaagctttt ggcgctacta aaaaagatgg tttgttatat tttaatgata taccaataga
    52141 tgaaagttgt gttgaactca tatttgatga taatacagcc aataagatta aagatagttt
    52201 aaaaactttg tctgataaaa agatatataa tgttaaactt gaaagaggaa tgaatatact
    52261 tataaaaaca ccaacttact ttactaattt tgatgattac aataattacc ctagtacatg
    52321 gagtaatgtc aatactacga atcaagatgg tttacaaggc tcagcaaata aattaaatgg
    52381 tgagacaaag attaaaatac ctatgtctaa gctaaaacct tataaacgtt atgtttttag
    52441 tggatattca aaggatcctt taacatctaa ttcaataatt gtaaagataa aagcaaaaga
    52501 agaaaaaacg gattatttgg taccagaaca aggatataca aagtttagtt atgaatttga
    52561 aactactgaa aaagattctt ctaatataga gataacatta attggtagtg gtacaacata
    52621 cttagataac ttatctatta cagaactgaa tagtactcct gaaatactta atgaaccaga
    52681 agttaaaatt ccaactgacc aagaaataat agatgcacat aaaatatatt ctgcagattt
    52741 aaattttaat ccaagtacag gaaatgctta tataaatggt atgtatttta caccaacaca
    52801 aactaataaa gaagctctcg attatatcca aaaatataga gttgaagcta ctttacaata
    52861 ttctggattt aaagatattg gaactaaaga taaagaaatg cgtaattatt taggagatcc
    52921 aaatcaacct aaaactaatt atgtcaatct taggagttat tttacaggtg gagaaaatat
    52981 tatgacatac aagaaattaa gaatatatgc aattactcca gatgatagag agttattagt
    53041 tcttagtgtt gattagtatc aaatgattta aatttgtccc cacaatacaa tatatattta
    53101 ccttataaag tactaaaaaa ttaactttat aaggtatttt ttatttaaat attctcttct
    53161 tctaatatat tcttttttag aactatattg tattataaag ataattatct atatcatatc
    53221 atccatatat aaacataaat tcatttaatt atataaattt taaaaaattt tttttgatat
    53281 attattgaca attttcatta taagagttaa aattaactca taaattacat attgggtata
    53341 aaatacaatg tatgttagaa gttgtttcag aaggctatgg ttttctgatt tttttatgct
    53401 cagtagtgat atgcaaaaaa gacaaagttg acatcagtta acttttacct ttattctact
    53461 ttccccaaag taagaataat ctaacccctt tgagatgagc aaaagctcat ctttttttat
    53521 tatcaaaagg tactgtctca aaatagaaat aatcctcttt ttcattgttg aaatgattaa
    53581 aatatatttt atttcataca aaaaagagtg cctcatgaac aaaagtttac tttgagactc
    53641 tctctttcat atcatatact tttttaataa ctttaaaatt tatttatcaa gtaaggatag
    53701 ctaataatta gtatcttctc tctataaatc ccactttttt ataaactttt ctaagagttt
    53761 ttctagcttg tttttcagct ttttctgctc ccatactata tattttttct aaataatctt
    53821 tattattaag aaggtcaaca tatttttctc ttataggtct taaagcttct gatactactt
    53881 cagccacatc ttctttaaat atgccataac cctttccttc atacatgttt acaatctctt
    53941 ctacactctt tttacttaaa ttagaatata tatcaattag atttttaata ccaggttgtt
    54001 catcattgta ttttacaaca cctaaagaat cagtaacact tctttttatc tttctcttta
    54061 tagagtctga atcatctgct aataatataa atgcattttc atttgaatca gatttactca
    54121 tttttttagt tggctcttgc aaactcatca ctcttgctcc accttttggg tagtatcctt
    54181 ctggaactac aaaagtaggt gaaaatctat tattaaacct atttgctaaa tcacgagcta
    54241 actctaaatg ttgcttttgg tcatccccaa ctggaactag gtcagtttga tacaacaata
    54301 tgtcagcagc cataagcact ggatatgtga ataaacctgc atttagattt gcttcactct
    54361 tttgtgattt atccttgaat tgagtcattc tacttaattc acccatataa gtcatagtat
    54421 ttaataccca cataagttca acatgctctt tgacatgtga ttgtataaat atagtatttt
    54481 tttctggatc taatccacaa gctatatatt gagcaagcaa ctctatagca ttagctctta
    54541 aaactttagg gtcctgaggt actgttattg catgcaaatc aactatacta taatagcaat
    54601 cataattatc ttgaagttca gtccagtttt ttatagctcc taaataattt cctaaagtca
    54661 ttttgcctga tggctgtgct ccactaaata taactttttt ttcactcatt tttttgtacc
    54721 tccttatata tttatattga ttttaacata tttaaaacta tgtctttaga attattagca
    54781 gcatcatgta caaatacatc ataagtaaca tcagcacttc catctgcctt atcagacatt
    54841 gctcttatta ttacgaatgg agtattattt aaatagcata catgagctat agctccacct
    54901 tccatttctc cacaatatcc accaaaatca tttacgagtt cttcttttaa ttctttagaa
    54961 tttataaatt tatctcctgt aactactctt ccttttaata ctttatgagt ctttacttct
    55021 tccacagaag atttgtaagc agcatctata agtctttcat ctgctttaaa tacagaagtc
    55081 ttcattcttg gtataactcc cttagggtct ccaaatgctg tagtgtctac atcatactgt
    55141 atagcatcag ttgatattac tatatcatta acatcaagtt tttcatttaa agctcctgca
    55201 acacctgtat taacaatagc atcaacctta aattcgctta ttagtatctg ggcacaaaga
    55261 gcagaattaa ccttacctat accacacttt actaatacta cattcttacc ttccaaaatt
    55321 cccttataaa attctaagct agcttttttt atagtctctc taatatccat taaatcaact
    55381 aaaatactaa cttcttcatc cattgcacct ataattccta ttacgttcat ttttcctcct
    55441 aaattttcta tattaaaaaa tccgtcctaa atcaatagga cggatatatt ttccgtggta
    55501 ccacctaaat ttatatacac tcaaaattga atatatatat cttaatatat gctataacgt
    55561 gcatacacga tatcaaatac tctgtaatct taaattacat ttcattttac tcctcaaaga
    55621 cccattcaat aaatactttt tattaagttc tcagcatcct taattctctg tgcaaaaata
    55681 tatttattta ctatttctta tctcaggatt taatttatta atttctttat tttataatat
    55741 tccttacact ataaattgtc aactatatat tataatatcc aaaagaaatt tttaaaatac
    55801 attttattta gctgttttct tttcatatct attccataac catagaccag ataatataac
    55861 tagaagacct ccaccaatca tctctatctc atatataaca ttttcagcaa atgtaggcat
    55921 aactggtgct gcagataata atataccaac tacagttaca actaaaacca tttttgctaa
    55981 acctaggcaa gtacttgttg attttgccat agtataaggt ctaatttcat ctggtctctc
    56041 ttttcttagt ttaatataag aagcatataa taatacatat gggaataatg cagttaatgc
    56101 tgtcatagtt acaagaacat tgtatatagc atcaacagaa ggcattaatg tagtcaccaa
    56161 tagtattata gtaactatta ttgcttgaag tataactgct ttagaaggta tcttgtgttc
    56221 atttacctct accaaactat ctggaaatat tcctttttta acactaccaa ataacatttt
    56281 tattggtgac gctatgtaaa gaactattgc tcctaatact gaaaaagcta taccaaaagc
    56341 tattaattga accaaccaag aaccaattcc taaagacgca caaacagcag aaagtgcatc
    56401 taatacacct ttagaagcag taatttggtc tggtggtatt atcattgtta ttgcaattga
    56461 acctaataca tataatgaag ctactattcc agctgatatt aatattgcct tagggaagtt
    56521 cttttcaggt ttatctattt ctgttatgaa gttagcagca gtctctgctc ctgctaaacc
    56581 aaacattata gctgatatag caacaaaggc atccatatta atttttggag tcattgaagc
    56641 tattgtgtat gtagaagcta tatcatgttt tttcaataca aatactgcta caaatgaaaa
    56701 tattataagt atagcacttg gtattgttga acctaatgca cctgtattag taaatatttt
    56761 tgtaaaactt attcctttag ttgctataag agataatgcc cagaatatta ttaaagataa
    56821 tataagtaca aacatcttgt tatcagcaag agctggattt cctattgtat aagaaacatt
    56881 tactgctaag aaagttaaaa atgaagaata ccaaaatatt ttagctgtcc agtttaacca
    56941 agaaactaaa aatccatact tttctccaaa ggcttcttta acccaatcgt aaagacctcc
    57001 atcacctttt tgatatgtag aagctaattc agcacaaatt aaagctcctg gtacaaagta
    57061 tataaatgag aataataacc acataggtat tgcacctaat cctaaaccaa agcttgatgc
    57121 tgtagactta gctatccatc ttataccata caaagctgat acattcataa ataccaaact
    57181 ccataggcct atttttttct tttccatcct aatttaattt ctcctctcca ttaattgttt
    57241 taaatttaat ttcaaaacta ataaaataga ttcattatta ttaaactgat atttttcaaa
    57301 atattttcat gttcaaaaac aattttaata ataaatttta aatactttga aatcaaatct
    57361 tattaaaaat gataaattta aaagttttac cactttaagt cta
//
LOCUS       DAMJVM010000014        54552 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.014, whole genome shotgun sequence.
ACCESSION   DAMJVM010000014 DAMJVM010000000
VERSION     DAMJVM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 54552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54552
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.014"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            3..79
                     /locus_tag="PC523_000924"
     tRNA            3..79
                     /locus_tag="PC523_000924"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Asp,seq:gtc)
     gene            90..164
                     /locus_tag="PC523_000925"
     tRNA            90..164
                     /locus_tag="PC523_000925"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:122..124,aa:Gly,seq:gcc)
     gene            174..247
                     /locus_tag="PC523_000926"
     tRNA            174..247
                     /locus_tag="PC523_000926"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:208..210,aa:Arg,seq:tct)
     gene            1158..1997
                     /locus_tag="PC523_000927"
     CDS             1158..1997
                     /locus_tag="PC523_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDF2474478.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            1994..3175
                     /locus_tag="PC523_000928"
     CDS             1994..3175
                     /locus_tag="PC523_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474479.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMNDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVHGTIEDPIQGQKEVY
                     LKANIPQGVTIEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKKNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVNLNDNKDNDALKNYKLSKEQVTIKGKKEDIDKINYIKTKPIDLADLANGE
                     EKEVSLEVPEGITLEGASITIKLNDTKQLSAEFLYSAGDIELRNIPTNMDNPSVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            3479..4381
                     /gene="ptb"
                     /locus_tag="PC523_000929"
     CDS             3479..4381
                     /gene="ptb"
                     /locus_tag="PC523_000929"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HDF2474480.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            4403..5482
                     /gene="buk"
                     /locus_tag="PC523_000930"
     CDS             4403..5482
                     /gene="buk"
                     /locus_tag="PC523_000930"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2474481.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            5472..6149
                     /locus_tag="PC523_000931"
     CDS             5472..6149
                     /locus_tag="PC523_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2474482.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            6192..6407
                     /locus_tag="PC523_000932"
     CDS             6192..6407
                     /locus_tag="PC523_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2474483.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            6436..7515
                     /locus_tag="PC523_000933"
     CDS             6436..7515
                     /locus_tag="PC523_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2474484.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGRLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            7515..8267
                     /locus_tag="PC523_000934"
     CDS             7515..8267
                     /locus_tag="PC523_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2474485.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            8268..8825
                     /locus_tag="PC523_000935"
     CDS             8268..8825
                     /locus_tag="PC523_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2474486.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            8997..10343
                     /locus_tag="PC523_000936"
     CDS             8997..10343
                     /locus_tag="PC523_000936"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDF2474487.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCMPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQSAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSELASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            10610..12442
                     /gene="glmS"
                     /locus_tag="PC523_000937"
     CDS             10610..12442
                     /gene="glmS"
                     /locus_tag="PC523_000937"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDF2474488.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEEEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELVSEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIVLDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKDIPSKIQEIL
                     DNEEYIKEVAKTVVNSDHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            12934..14229
                     /locus_tag="PC523_000938"
     CDS             12934..14229
                     /locus_tag="PC523_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HDF2474489.1"
                     /translation="MANDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDKKDEVLEQALKTKRIDIDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADNENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFENSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGFTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFTSNPK
                     HSCTNFGADIPVNSQLVVTTSELRQKLKEKNKFVLVDNRTWDEYIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            14700..15437
                     /locus_tag="PC523_000939"
     CDS             14700..15437
                     /locus_tag="PC523_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HDF2474490.1"
                     /translation="MKRVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNIKINSVINHNLSNKEFYNIFIEIINNLKLDKKGINWNRKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGRTLIYIATPIIKLDY"
     gene            15458..16711
                     /gene="murA"
                     /locus_tag="PC523_000940"
     CDS             15458..16711
                     /gene="murA"
                     /locus_tag="PC523_000940"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDF2474491.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            16798..17862
                     /gene="spoIID"
                     /locus_tag="PC523_000941"
     CDS             16798..17862
                     /gene="spoIID"
                     /locus_tag="PC523_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HDF2474492.1"
                     /translation="MKNPLIVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIKDKSNKEEKNIVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            17928..18596
                     /locus_tag="PC523_000942"
     CDS             17928..18596
                     /locus_tag="PC523_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDF2474493.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFNDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVNVKKEQKITEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            18694..18954
                     /gene="spoIIID"
                     /locus_tag="PC523_000943"
     CDS             18694..18954
                     /gene="spoIIID"
                     /locus_tag="PC523_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HDF2474494.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            19151..20170
                     /locus_tag="PC523_000944"
     CDS             19151..20170
                     /locus_tag="PC523_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDF2474495.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            20186..20614
                     /gene="fabZ"
                     /locus_tag="PC523_000945"
     CDS             20186..20614
                     /gene="fabZ"
                     /locus_tag="PC523_000945"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDF2474496.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(20725..21234)
                     /gene="yyaC"
                     /locus_tag="PC523_000946"
     CDS             complement(20725..21234)
                     /gene="yyaC"
                     /locus_tag="PC523_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HDF2474497.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     regulatory      21423..21526
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            21662..22855
                     /locus_tag="PC523_000947"
     CDS             21662..22855
                     /locus_tag="PC523_000947"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDF2474498.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGIVDYLRPDGKTQVTVEYEGNKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEDTKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVASGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     gene            23168..24265
                     /locus_tag="PC523_000948"
     CDS             23168..24265
                     /locus_tag="PC523_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474499.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     VGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGIFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCILYRRITKTEGYKENKSEIS"
     gene            24529..26748
                     /locus_tag="PC523_000949"
     CDS             24529..26748
                     /locus_tag="PC523_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDF2474500.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKVYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIDVINKNPYQLAEDIRGIGFKVADSIANKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELSGKIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEENKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMISSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYVLFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPITWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            26751..27533
                     /locus_tag="PC523_000950"
     CDS             26751..27533
                     /locus_tag="PC523_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDF2474501.1"
                     /translation="MKICNDNFIKDFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIHHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKIVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYVLFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENIKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            27865..29778
                     /locus_tag="PC523_000951"
     CDS             27865..29778
                     /locus_tag="PC523_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2474502.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNEIANDFECSERTIRNDCKY
                     IDEWLSTFCNACIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEIGKLSVALNNYNLNISTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMFTIEENMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSSNIKKSLYLNEELANYTRKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            29919..30227
                     /locus_tag="PC523_000952"
     CDS             29919..30227
                     /locus_tag="PC523_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2474503.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            30252..30578
                     /locus_tag="PC523_000953"
     CDS             30252..30578
                     /locus_tag="PC523_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2474504.1"
                     /translation="MKRKVYLFCSFGMSTSLLAEKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            30618..31952
                     /gene="celB"
                     /locus_tag="PC523_000954"
     CDS             30618..31952
                     /gene="celB"
                     /locus_tag="PC523_000954"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDF2474505.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLISSPLLIIGSLFLLV
                     SNFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSVHEFIFKFL
                     QIPLLKLGNTLSATAIAYVFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGT
                     KLPNIITAQFQDMFATFGGAGSTLSLIIAMLLICKSKRIKSIGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFALVPTVNIIIAYFCMSTGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLIILGIFMYIPFIKMLDKQYLKDEKSNVEGEDDDISFDDLVL"
     gene            31963..32481
                     /locus_tag="PC523_000955"
     CDS             31963..32481
                     /locus_tag="PC523_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HDF2474506.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEPFLNE
                     SKVIATLFCGDEFFINNEEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKADIPAFAAMSEENIDVINEYKNDICIVRTPKKGGIGLNDSLNNICKLAKAMANK
                     EDITLMKEEFCY"
     gene            32577..33536
                     /locus_tag="PC523_000956"
     CDS             32577..33536
                     /locus_tag="PC523_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HDF2474507.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEEYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            33585..34637
                     /locus_tag="PC523_000957"
     CDS             33585..34637
                     /locus_tag="PC523_000957"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDF2474508.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKIRNLNNYIGYAEHGDSEDYVCVIGHVDVVHEGDGWKYQPYKGE
                     EINGRIYGRGVLDNKGPIISALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVLLGFIENVSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKFSFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            34640..35314
                     /locus_tag="PC523_000958"
     CDS             34640..35314
                     /locus_tag="PC523_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDF2474509.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIERV
                     VNSVKIPVNVMIRPHAKSFVYSKEDINIMQKDVSVVKEIGANGVVFGVLDKNNNIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKTLKDYDKIINILTSGGKGSIVDNIQIIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRMNNSPFEDIDRQKLRQL
                     VNIISR"
     gene            35867..36418
                     /gene="raiA"
                     /locus_tag="PC523_000959"
     CDS             35867..36418
                     /gene="raiA"
                     /locus_tag="PC523_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDF2474510.1"
                     /translation="MNIIISGKQIELTDAIKNKIESKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            36550..39225
                     /gene="secA"
                     /locus_tag="PC523_000960"
     CDS             36550..39225
                     /gene="secA"
                     /locus_tag="PC523_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDF2474511.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPISKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            39240..40347
                     /gene="prfB"
                     /locus_tag="PC523_000961"
     CDS             join(39240..39314,39316..40347)
                     /gene="prfB"
                     /locus_tag="PC523_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDF2474512.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNKQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEEDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            40492..42633
                     /locus_tag="PC523_000962"
     CDS             40492..42633
                     /locus_tag="PC523_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDF2474513.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAENYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKENFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVNSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DREVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHIAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIDEIDEKIKEIGLKELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMILKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            42781..43947
                     /locus_tag="PC523_000963"
     CDS             42781..43947
                     /locus_tag="PC523_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2474514.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIEEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPVDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTYGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIADELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKTLGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVIYTIINGKVVYEKK"
     regulatory      44007..44124
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            44248..44853
                     /locus_tag="PC523_000964"
     CDS             44248..44853
                     /locus_tag="PC523_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2474515.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     gene            45183..46223
                     /locus_tag="PC523_000965"
     CDS             45183..46223
                     /locus_tag="PC523_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2474516.1"
                     /translation="MNLSKKIKKLFLDDKGYYFNSNWKDIHIGIYDTKLGDSLKYITN
                     EYIENLERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKSQTEELCLEAIKYNYK
                     ALEYVKEQTEYICLEAIKKDCNALKYVRNKTEGLIIKVIRHSTNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVENQTEKMCIEAVKQNGMA
                     LQYVNKQTDKICIEAVKQDGRSLQFVDNKTEEICINAIRYLNKKYNIKDVLCYVDNYT
                     EDMCIEIVRQNGKMLMYIKNQTEKICIEAVKENYKSLKYVKEQSERICKEALKQNYKA
                     KEYVKIAIDDCI"
     gene            46362..46814
                     /gene="tsaE"
                     /locus_tag="PC523_000966"
     CDS             46362..46814
                     /gene="tsaE"
                     /locus_tag="PC523_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDF2474517.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIKDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            46814..47530
                     /gene="tsaB"
                     /locus_tag="PC523_000967"
     CDS             46814..47530
                     /gene="tsaB"
                     /locus_tag="PC523_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDF2474518.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTANNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     MNFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVIEFDALVEEIVSTNEEFIM
                     VGEAVYKYKDKLENIENIKIPSPANNVSKAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            47520..47996
                     /gene="rimI"
                     /locus_tag="PC523_000968"
     CDS             47520..47996
                     /gene="rimI"
                     /locus_tag="PC523_000968"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDF2474519.1"
                     /translation="MEDNINLIVNDIKIEEMTIEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLDGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            48002..49018
                     /gene="tsaD"
                     /locus_tag="PC523_000969"
     CDS             48002..49018
                     /gene="tsaD"
                     /locus_tag="PC523_000969"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDF2474520.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANMDFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAQKGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDSGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            49315..52023
                     /gene="hpdB"
                     /locus_tag="PC523_000970"
     CDS             49315..52023
                     /gene="hpdB"
                     /locus_tag="PC523_000970"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HDF2474521.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLNEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTHHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKSVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLREDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDYRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            52027..52284
                     /gene="hpdC"
                     /locus_tag="PC523_000971"
     CDS             52027..52284
                     /gene="hpdC"
                     /locus_tag="PC523_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HDF2474522.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDEACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            52277..53227
                     /gene="hpdA"
                     /locus_tag="PC523_000972"
     CDS             52277..53227
                     /gene="hpdA"
                     /locus_tag="PC523_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HDF2474523.1"
                     /translation="MSSQKKLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKAYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSSEVFNKIFEDIDFAFIDIKHMDREKHKEQTGVYNDLILKNIS
                     NLANSDWNGRLVLRVPVISGFNDSVKNISDIISFMHKNNLVEINLLPFHRLGESKWVQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            complement(53335..53562)
                     /locus_tag="PC523_000973"
     CDS             complement(53335..53562)
                     /locus_tag="PC523_000973"
                     /inference="COORDINATES: protein motif:HMM:NF017000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474524.1"
                     /translation="MYKKTNDNVYLYNLEKTLDEIFQLFDLSLRYSFKTFSNLETMEN
                     TSFLEGTSGIILLLISISSKDSTYKKLLFLS"
     gene            complement(53591..54385)
                     /locus_tag="PC523_000974"
     CDS             complement(53591..54385)
                     /locus_tag="PC523_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2474525.1"
                     /translation="MMILPLSSVAIAKFYTEGLNNDMYKFYSLIILFFLIYSLLIILR
                     LLNLINNSEFTIINLILTFISSLSIIIYSSNTKNLYPVKNIKAGSLLIIYFMISVIVP
                     TSFLLILQGTQPSYSGIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFDRFGKKLG
                     VIILGMCWSLWHLPLIFTLYTPETPILGLILRSIHIVGIAIFLGYLYMKTKNVWFCAI
                     IHILNNSAFLITNVEESIITYSDILIVSMVSLIFYVPFLFTKEYKG"
BASE COUNT        21989 a         5813 c        10337 g        16413 t
ORIGIN      
        1 gcggcctggt agttcagttg gttagaatgc cagcctgtca cgctggaggt cgagggttcg
       61 agccccttcc aggtcgccaa attaaataag cgggaatagt tcagtggtag agcgcaacct
      121 tgccaaggtt gaagtcgcga gttcgaatct cgtttcccgc tccataataa tttgggtgca
      181 tagctcagct ggatagagca acgcccttct aaggcgtgtg tccggggttc gaatccctgt
      241 gcgctcatta agaaaagttt actataattt atagtaaact tttatattgt ttaaaaagac
      301 acaggtaata tacatgtatc ctgtgtcttt ttatttataa actttaaact atgtaaaaat
      361 tatagtatat tctaatagag ttacagtata ttcattaatg ttgtaaacat atgaagggta
      421 atgtataata gtctacaagg tgtttatgaa ggcaatagtt aaatatgact aattggatag
      481 aaactatata cattatattg tatactttat ggtatttata aagagattca ttttgcctaa
      541 acaataagta taagaaatta tacaaaaatt agatactata atggattgac aatttaatgt
      601 aatgtttata agttatacta aataaattat tatactgtga agtatatata actttaggta
      661 ttgtgtatta ttttcactgt agaagattct atctagttat acatgatgag ttttaatgat
      721 agtttatagt aagatattaa aatatattta tctaatctag atatgaaatg agttattact
      781 ttaatataaa aatgaaccta atgaaacaat attgatgata agtaatactt aaattttgta
      841 tctgtatata gttgataaat gtattataga atttacaata attaatatat tttagagaat
      901 aaaattgcat taaggcgcga gtactacttt gtattcgcgc cttgatagct tgaattattt
      961 aagtaaaagt aattttaata aaaaataagt atttattaag ttgatatttt taatgtgttt
     1021 agagattatt taacaatctc taaatataaa actagaatta tttacataaa agttaaatag
     1081 tttgtataga gttagattta tatagtataa tacttagtgt aaataattaa gaagttatca
     1141 aacaggagga taaaactgtg ttagatataa attctttttt aaatggattt tttaatataa
     1201 tattaagaat gagtatatat gatttaattg acatatctat agtagcatat attttttaca
     1261 aaatatttat gtttataaaa gatacaagag cagaacaagt gtttaaaggt ataatatttc
     1321 ttttattagc aactcaactt agtaacacct ttaaattgca taccgtttat tggatatctc
     1381 ttaaggcttt agattatgga gttatagctg ctcttataat ttttcagcca gagtttagag
     1441 caggacttga acatatagga agagcaaagt ttaatttatt tggaaagaat gtaaatactt
     1501 ctgaagaaac tctcaataga aatatagaag aaattgtaga agctttatat tcactttcta
     1561 gacaaaaaat aggtgctctt atcataatgg agagagaaac tagaataagt gatataataa
     1621 atacaggaac tattattgat gctgaaattt ctaggcaact gttaataaat atatttatac
     1681 caaatacacc attacacgat ggagctgttg taattagaga ttctaaggta aaagcagctg
     1741 catgtttttt gccattgaca gaaagtaagg atttaagtaa agatttgggg actagacata
     1801 gagcaggaat aggagtaagt gaggtgtctg actgtataac cttgattgta tcagaggaaa
     1861 caggtggcgt atcaattgct aaagcaggta agttatatag agatatttct agagaaagaa
     1921 tgatgaatat attacgtagt aatttaaaaa caaatacaga aacaagaagt tttttcaaag
     1981 gtggtatatt taaatgataa acagattaaa aaataatacc aaaataaaaa taatatcatt
     2041 tttaagtgca attgcattat ggatgtatgt tatggctgta gttgaccctg aagaaactaa
     2101 agggtttgaa gatatacctg tatctataag taatatgaac gatttgaagg ataaaaatct
     2161 tgttatatat ccagacgaaa aattaacagc agatgtatat ataaaaggta gtttatcaaa
     2221 ggtacaaagt gttaagaaag aagatataca tgtacatgga actattgaag acccaataca
     2281 aggacagaag gaagtgtatt taaaagcaaa tattcctcaa ggtgttacta tagaatttaa
     2341 gcatgatgtt ttagtagtga atcttgaaaa aaatataaaa gaaaaaagag atataaaggt
     2401 agaagtaagt ggaaattcaa aaaaaaatat aaataaaatt gaacttagta aggaatttgt
     2461 cgatgtgtcg ggtccaagaa ctctagttaa agaagtcaaa agtgtaagag ctaatttaga
     2521 tgttggtaat gagacagatg atttttctaa aaaattgcaa ttaataccat taaatagtga
     2581 tggaactaaa gttgaaggag taacattaag ctcatctttt gtgactgcaa aaatagaact
     2641 gttaaaggaa aaaattgtcc caataagagt aaatctaaat gataataaag ataatgatgc
     2701 actgaaaaac tataaattaa gtaaggaaca agttacaata aaagggaaaa aagaagatat
     2761 agacaaaata aattatatta agacaaaacc tattgactta gctgatttgg ctaatggtga
     2821 agaaaaagaa gttagcttag aagttccaga gggtataact cttgagggtg cctctataac
     2881 aataaaatta aatgatacaa aacaattgag tgcagagttt ttatatagtg caggagatat
     2941 tgaattaaga aatatcccaa caaatatgga taatccaagt gtaatttctg atgtagatat
     3001 aaaagttgtt gtagagtcaa cagaagattt gagtaagtta aataaaaaag atatttcatt
     3061 atatattgat ttatcacaag gtcctgagga agatagtaaa tataaaataa aatatgaaac
     3121 aacatatcaa tttaaaaaaa taaatataga acctaatatt gttgaagtgg aatgaacaaa
     3181 tatttcgaat atttagttgt tacaagatgc gacaaaaaaa cctataaaat taagtttgtt
     3241 aattttatgt ttttgttgca tcttgtttat tttagttata ttataaaata aggacaatga
     3301 gatatttgtt aaatctaaaa agcgctagat atatgaaaaa agactgataa atagatttaa
     3361 ataaatattt tgaattaaat atttcaatta tctctaggaa aacgttgctg aacgagttat
     3421 aatttaattg agatataaaa agttctattt caaaaaattc aagatgaggt ggaaatgcat
     3481 gagaagtttt gaagaagtaa ttaagtttgc aaaagaaaga ggacctaaaa ctatatcagt
     3541 agcatgttgc caagataaag aagttttaat ggcagttgaa atggctagaa aagaaaaaat
     3601 agcaaatgcc attttagtag gggatataga aaagactaaa gaaattgcaa aaagcataga
     3661 catggatatc gaaaattatg aactgataga tataaaagat ttagcagaag catctctaaa
     3721 atctgttgaa ttagtttcac aaggaaaagc cgacatggta atgaaaggct tagtagacac
     3781 atcaataata ctaaaagcag ttttaaataa agaagtaggc cttagaactg gaaatgtatt
     3841 aagtcatgta gcagtatttg atgtagaggg atatgataga ttatttttcg taactgatgc
     3901 agctatgaac ttagctcctg atacaaatac taaaaagcaa atcatagaaa atgcttgcac
     3961 agtagcacat tcattagata taagtgaacc aaaagttgct gcaatatgcg caaaagaaaa
     4021 agtaaatcca aaaatgaaag atacagttga agctaaagaa ctagaagaaa tgtatgaaag
     4081 aggagaaatc aaaggttgta tggttggtgg accttttgca attgataatg cagtatcttt
     4141 agaagcagct aaacataaag gtataaatca tcctgtagca ggaagagctg atatattatt
     4201 ggctccagat attgaaggtg gtaacatatt atataaagct ttggtattct tctcaaaatc
     4261 aaaaaatgca ggagttatag ttggggctaa agcgccaata atattaactt ctagagcaga
     4321 cagtgaagaa actaaactta actcaatagc tttaggtgtt ttaatggcag caaaggctta
     4381 attttaaggg ggaaataggc aaatgagcaa aatatttaaa atcttaacaa taaatcctgg
     4441 ttcgacatca actaaaatag ctgtatttga taacgaggat ttagtatttg aaaaaacttt
     4501 aagacattct tcagaagaaa taggaaaata tgagaaggtg tctgaccaat ttgaatttcg
     4561 taaacaagta atagaagaag ctctaaaaga aggtggagta aaaacatctg aattagatgc
     4621 tgtagtaggt agaggaggac ttcttaaacc tataaaaggt ggtacttatt cagtaagtgc
     4681 tgctatgatt gaagatttaa aagtaggagt tttaggagaa catgcctcaa atttaggtgg
     4741 aataatagca aaacaaatag gtgaagaagt aaatgttcct tcatacatag tagaccctgt
     4801 tgttgtggat gaactagaag atgttgctag aatttctggt atgcctgaaa taagtagagc
     4861 aagtgtagta cacgctttaa atcaaaaggc aatagcaaga agatatgcta gagaaataaa
     4921 caagaaatat gaagatataa atcttatagt tgcacacatg ggtggaggag tttctgttgg
     4981 agctcataaa aatggtaaaa tagtagatgt tgcaaacgca ttagatggag aaggtccttt
     5041 ctctccagaa agaagtggtg gattaccagt aggtgcatta gtaaaaatgt gttttagtgg
     5101 gaaatatact caagatgaaa ttaaaaagaa aataaaaggt aatggtggat tagttgcata
     5161 cttaaacact aatgatgcta gagaagttga agaaagaatt gaagctggtg atgaaaaagc
     5221 taaattagta tatgaagcta tggcatatca aatctctaaa gaaataggag ctagtgctgc
     5281 agttcttaag ggagatgtaa aagcaatact attaactggt ggaatcgcat attcaaaaat
     5341 gtttacagaa atgattgcag atagagttaa atttatagca gatgtaaaag tttatccagg
     5401 tgaagatgaa atgattgcat tagctcaagg tggacttaga gttttaactg gtgaagaaga
     5461 ggctcaagtt tatgataact aatgataaaa gaataagaat cataactggt cactatggaa
     5521 gtggtaaaag tgaatttgcg atgaactatg ttgttaagtt aagagatatg gtttctggca
     5581 aagttgctat agccgattta gatgtggtaa atgtatactt tagaactagg gaaaagaaag
     5641 agttaatgaa atctttaggt atacaaccaa tagatagctc aattaatgct ccaacattag
     5701 atttaccagc agtatcagca gaggtgatgt ctcctatggt ggaccattca tacaattctg
     5761 ttatagattt aggtggagat aatgtaggtg ctagggttat aggaagattt agtcatttgt
     5821 taaaagaagg cgactatgat atgctttttg taataaatgc aaatagggag aaaactcaaa
     5881 cttctgaaga agttatccaa tatataaaag aaatagaaaa atcttctaaa ttaaaggtaa
     5941 caggtctaat taataatact catcttatta gatttactac tattgatgat gttttaagag
     6001 gtcagaaagt tgcaaaagaa gtttctgaaa aatgtaatat accaataaga tatgttgcat
     6061 gtttagaaaa tcttgttgaa cagttaccaa aagatttaga aggagaaatt tttcctataa
     6121 aattatatat gagagaagac tggatgtagt ttgagctcaa atatatagaa agtttaagga
     6181 ggcttaggta aatggctaaa ggaaaagtat cttttaatca agaacgttgt aaaggttgtg
     6241 gattgtgtgt tgaagcatgt ccagtaaaaa tcatacaatt agactcaaat gtaataaaca
     6301 aaaaagggta taacccagca actgtttttg aaatggaaaa atgcatagga tgtgcaagtt
     6361 gtgcaactat gtgtccagat gtagttataa cagtagaaag agattaattt aattaatatt
     6421 taagggggaa ttttaatggc taagatactt atgaaaggta acgaagcatt tggaaaagca
     6481 gctatagaag ctggttgcaa atatttcttc ggttacccaa taactccaca aagtgaatta
     6541 ccagaatatt tatcaagaga gttacctaaa ataggtggag cgttcgttca agctgaatca
     6601 gaagtttctg cgataaacat ggtttatggt ggagcaggtg caggagcaag agttatgact
     6661 tcttcatctt caccaggagt tgctttaaag caagaaggaa taacatatgc agtaggagca
     6721 gaagttcctt gtgttgtact taatgtaatg agaggtggtc caggacttgg aagtatacaa
     6781 ccttcacaag ctgactactt catgtctaca agaggtggag gaaatggaga ctacagaact
     6841 ccagtattcg caccagctac agttcaagaa gctgttgata tgataatgga agcttttgat
     6901 gttgcagatt attacagatc accagttatg gtagttgcag atggtatgat aggtcaaatg
     6961 atggagccag ttgaatttag agctcctgag aagaaaagag aattgcctcc taaagattgg
     7021 gcaactgttg gaactaaagg aaaaagaaaa cctaatgtaa taaactctct atacttagag
     7081 ccagaagtac tagaagacca ttgttggcat ttacaagaaa aatttgatgc aatggaaaaa
     7141 aatgaagtac aatatgaaat gtacaaaaca gaagatgctg aatttgtatt tgcagcttat
     7201 ggaactactt caagagtagt aaaaagtgca attgatatat taagagaaga aggaataaaa
     7261 gcaggtctta taagacctaa agtattatgg ccattcccat tcgaagcatt caaccaaatt
     7321 cctaatgcta gaaacatatt gactgttgaa atgagtatgg gacaaatggt ggaagatgtt
     7381 aaaatggcag tagaaggtag attaccagtt tacttccatg gaagaccagg aggaatgact
     7441 ccaacaccag cagaaatagt tgaaaaagct aaaaaaatta ttgccggaga attggttgca
     7501 ggaggtgcta gataatggct gttgtattta agaaaactga aggattacaa gatactcaaa
     7561 ctcactactg tccaggatgt acacatggta taatccatag attagttggt gaagtgttag
     7621 aagaactagg agtattaggt gatgctgtag gtgttgttcc agttggatgt tcagttttag
     7681 gatataaata ttttaactgt gacactcaag aagctgctca tggtagagca ccagctgctg
     7741 ctacaggaat aaaaagagtt catcctgaaa atacagtatt cacttatcaa ggtgatggag
     7801 acttagcttc aatcggtact gctgaaatag ttcatgcagc tgctagagga gaaaaaataa
     7861 ctactatatt tgtaaacaat acaacttatg gtatgactgg tggacaaatg gctccaacta
     7921 cattagttgg acaaagagct actactgctc aatcaggaag aaatgctgaa actcaaggat
     7981 atccaataag agtaagtgaa atgttagcta cattaacagg agctgtattt gtagaaagag
     8041 tagcagttga tactccagct catgttagac aagctaaaaa agctataaag aaagcattcc
     8101 aagttcaaca agcaggatta ggatttggaa tagtagaagt attatctact tgtcctacaa
     8161 actggggact agctccaaat gatgctttac aatggttaag agacaatatg ataccatact
     8221 atccattagg aaatttcaaa aatgttgaag ttgaggaggt gaagtaaatg tctacagcta
     8281 gagtaatatg tgcaggcttc ggaggccaag gtgttatgtc tatgggacaa ttacttactt
     8341 atgcagggat gctagaagga aaagaagttt cttggttacc atcttatgga ccagaaatgc
     8401 gtggaggtac agcaaactgt gcagtaactg tatctaatga accagttggt tctccactta
     8461 taacagatga tgcaactgca gctatattat taaatatacc agcatttgaa aaattcaaag
     8521 atgatgttat tcctggtgga aaaataatag taaatagttc tttaataaaa gaaaaagttg
     8581 atagaacaga tgtagatgtt tattatatac cagcaaatga attagcagct gaattaggaa
     8641 atgataaagt tgctaatatg ataatgttag gagcttactt aaaagtatct gatactgtag
     8701 atatagaatc tgtactagaa gcattcaaaa aagttttcgg acctagaaaa gaaaaattcg
     8761 ttccgttaaa cagagaagct ttacaaaaag gtatggatgc tacatgctct aaagcaacta
     8821 actaataaag aagaaaagat gtcaaaaaaa agacaataga attatttcta ttgtcttttt
     8881 tgctgataag tattaaaata tttttatgac tgaaattaag cctataatga tattttatga
     8941 actataaaaa tataatacaa attacaagct tttcaattat gaaaggagaa tctttagtga
     9001 gaaagtattt tggaacagat ggagttagag gagtagcaaa tacagaacta acatgtgatt
     9061 tagcatataa acttggtaga gctggtgggt ttgtcttagc tcaaggagac cacagagtaa
     9121 aagtagtagt aggtaaggat actagaatat caggtgatat gttggaagca tctttaatag
     9181 caggacttat gtctgttgga tgtgatgtta ttactgttgg aataatacca acacctgcag
     9241 tggcatattt aactagaaaa tatggtgctg attgtggtgt ggtaatatct gcatctcaca
     9301 atcctgttga atataatgga atcaaattct ttaataagaa tggatataaa ttagatgatg
     9361 aaattgaatt gaaaatagaa gaatatatag atgatataga caaaattgat tgtatgccaa
     9421 ttggtgaaaa tgtaggaaga aaattacatg agcattgtgc acaaagagat tatgtagact
     9481 atttaaaatc aataataagc actgacttta aggggttaaa agtagtttta gactgtgcta
     9541 atggtgcgtc atataaagta gcaccaatag tgtttgatga attaggagca agtgtaattt
     9601 caataaatag ttcaccagat ggaaataata taaactataa atgtggctct acgcatccag
     9661 aacaacttca aagtgctgtt ttggaacata atgctgactt aggtcttgca tatgatggag
     9721 atgctgatag attaattgca gtaaatgaaa agggtcaaat tgttgatgga gaccatataa
     9781 tgatattgag tgccttaaat ttaaagaaaa ataataaatt agcacaagat actttggtag
     9841 ttacagttat gagtaatata ggattaacaa tagctgctaa ggaaaatgga ataaatttat
     9901 ctactacagc agttggagat agatatgttt tagaagatat ggttaaaaat ggatataatc
     9961 ttggtggaga acaatctgga catatgatat ttttagatta caacactact ggagatggag
    10021 tgcttagttc tctaatactt gcaaatataa tcttgcaaga aaagaaacct ttatcagaac
    10081 ttgcatcaat aatgagtcaa tatccacaag tattagtaaa tgcaacaata aaaaatgaaa
    10141 ataagaacaa atatatggaa tatccagaaa taaaaactga gatagaaaga atagaatcta
    10201 ttttagatgg aaatggtaga gttttgataa gaccatcagg aactgagcca ttagttagag
    10261 ttatgttaga gggtaaagag gaaggtcaaa taaaagaatt agcaacaaat ttagctaatc
    10321 ttatacaaga aaaattatcg taatttaaag ggtattctca gatataaata ctgaggacac
    10381 ctttctaagc gccagaactt agttatagac ataattattg agtaatatag gaactaagtt
    10441 gacgaggtca gagttaatcg aattgtcggc ggatgctctg cggtgtgttt accatcgaaa
    10501 gacttcttta aataatagga gtaatcctat ggacaaaggg aagccaaagt aaacttatat
    10561 gacctaactc ttaaaatata taaataatat ttttaggagg acacaaataa tgtgtggaat
    10621 agttggatat ttaggaagta gaaaggcagc agaagttata gtagaaggac tttctaagct
    10681 agagtacaga ggttatgact cagcaggagt agcagttaat agttctaatg aagaagaact
    10741 taatattaga aagtttaaag gtagactttc tgtacttgca gaagatttag aaaagaaccc
    10801 tattgatggg aatttaggga tagggcatac aagatgggca actcatggag aaccatctga
    10861 tgtaaactca catcctcatt ttaatcaagc caaaacaata gcagttgttc ataatgggat
    10921 aatagaaaac tatatggaaa taaaagaaga attagtttct gaaggtgtta aatttgaatc
    10981 tcaaacagat acagaggtaa tagcacattt agtagataaa tattatgagg gtaatttatt
    11041 agatgctgta tacaaaacta tatcaaaatt aagaggagct tatgcattag gtgttatatg
    11101 taaagaacat ggaaatgaat tagttgcagt aagaaaagat agtcctcttg tagttggtgt
    11161 tggtgaaggt gaaaacttta tagcatcaga catacctgca ttattaaaat atactagaga
    11221 tgtttatttc ttagaaaatg gagaagtagt tcatctaaaa gacgaaaatg taacagttta
    11281 tgactctaat agaaatcttg tggaaaaaga agtatttcat gtaacttggg atgtagaagc
    11341 tgcgtctaaa ggtggatatg actacttcat gtcaaaagaa atccatgagc aaccaacagg
    11401 agttagagaa actttagaaa gaagacttga tgataatgga aatatagtct tagacagtat
    11461 aaatatttct aaagaagatt tagaaaagat aaataaagtt tatatagtgg cttgtggaac
    11521 agcatataat gctggattac ttggtaagta tgctatagaa aaatttgtaa atataccagt
    11581 aataactgat atagcctcag aatttagata tagcgaccca tttgtagatg aaaattcatt
    11641 agtaatatta gttagccaat ctggtgagac agcagatact ctagcagtat taagagattc
    11701 aaaggctaaa ggagcaagaa tactttcaat aactaacgtt gtaggttcat ctatagcaag
    11761 agaatcagat gatgtattct atacttgggc aggacctgaa gtagcagttg catcaactaa
    11821 ggcatatact actcaaataa cttctttata catgatagct ttagactttg ctatcaaaaa
    11881 aggaacaata actagagaat tttatgatag tatgatttct aagatgaaag acataccatc
    11941 aaaaatacaa gaaatactag acaatgaaga atatataaaa gaagttgcta aaacagttgt
    12001 taattctgac cacgcattct atttaggaag aggtatagat tatagcttag caatggaagg
    12061 ttcattgaaa ttaaaagaga tttcatatat acatgcagaa gcttttgcag caggagagct
    12121 aaagcatgga acaatagctt taatagaaaa aggaactcct gtaattgcca tagctactca
    12181 agaaaagtta tttgaaaaaa tggtttctaa tatggaagaa gtaagagcaa gaggagctta
    12241 tgtagtagca atagctcaaa gccataataa ggatgttgag aaagctgctg ataaaataat
    12301 ctatatacca aattcagatg atattttatc accaatatta gcagttgtac caatgcaatt
    12361 acttgcttat catgtctctg tattaagagg atgcgatgta gataagccaa gaaatctagc
    12421 taaatcagta acagttgaat aaaaatagat taaaaattaa atagataata tttattgaaa
    12481 aaaactcatc ctgttatttg tattttatag gatgagtttt ttagataaat attaaatttg
    12541 ttttgttttt gctataataa attttataaa aagattgaat taaatcatct gtaaaatgat
    12601 aaatatatag accatataag gtatagagaa taataaatat atagttcata taaagtatag
    12661 tgagtactta ataaacatat agtcgatatc agatatagtg aatacttaat aaacagatag
    12721 tcaatataag acataaaaaa tactagatta tgagaaagat taaaatacat aaggtattag
    12781 taaccttgtg ataaaataaa gtgagtagtt taaaataaaa atataaggaa ctatttataa
    12841 aagaatataa tgaagctaat agagtataag aaaattactt tattttacta aaatgatttt
    12901 aaataaaaaa tttttaaatt gggagtataa attatggcaa atgattttaa aaaagaaaag
    12961 aaagaagatt attttgttaa tcttaaggca atatctactg aagttttaca agaaaaagtg
    13021 caagataacg cttgggttat agttgatact cgcttaaatg atgcttataa tggctggaag
    13081 ttagatggag taaaaagagg tggacatata aaaggagcag tagatttttc tgctaattgg
    13141 ttaagtgtat actcagataa aaaagatgaa gtgctagaac aagcattgaa aactaaaaga
    13201 attgatatag ataaaaatat agtattatat gatgcaaatg gaaaagatgc tttggttgta
    13261 gcagattatt tatctaaaaa aggatataaa tatctatata aatatgatat taaacaatgg
    13321 gcagacaacg aaaatcttcc aatggaaaga tataaaaatt atcaaatgat agttcctgct
    13381 tttataataa aagatatatt ggatggaaaa attcctgaga catttgaaaa ttccaagaat
    13441 ataaaaatga ttgaagcgag ttggggagaa gaatcgtata caaaagggca tatacctaca
    13501 agtgttcatg taaataccga tattatagaa cctcctccaa catggatgct tgataatgat
    13561 gataacttga ctaagtttgc tttagactat ggattcacaa aggatgatac tgtaatagtt
    13621 tcaagtagta cacctatggc atcttataga ctagctgtta tattgagata tataggagta
    13681 aaagatgtaa gagttttgaa tggcggaaca aactcatggt tatcagcagg atatgaatta
    13741 gaatttacta gtaatccaaa acattcatgt actaattttg gagcagatat accagttaac
    13801 tcacaattag ttgttactac atctgaatta agacaaaaac taaaagaaaa aaataagttt
    13861 gtactagtag ataaccgtac ttgggatgaa tatataggaa aagtatctgg atatacatat
    13921 tatgataaaa aagggcgtat accaggagct ttatatggac attcaggtag tgatagtgtt
    13981 tcactagaag aatatcgtaa tattgataat acaatgagaa ataaatatga gattctagaa
    14041 atgtgggaca aggaaaatat agatgtgaat aaacagctta tatttatgtg tggaagtgga
    14101 tggcgtgctg ctgaagtatt aacatacgca aatgtcatag gagtggaaaa tacctcactt
    14161 tatagtgatg gatggatggg ctggagtctt gataattcaa atttaattga agtgggagaa
    14221 cacaaataaa gtttttgaaa gtacgataga gttatttaaa acaaaggtgt ttttgattta
    14281 ttaggaagag tatgtaaatt gtgttttggg aagtgtactt aggtatgttt atttggagct
    14341 atggaaattg ataaaaaagt attatctgtt tattttgctg gtaaaatgtg aatagattat
    14401 agattataga ttatagatta tagattatag attatagatt atagatttag gtatagacta
    14461 agtaatttat attacagaag agaatatata agaactagtg caagatgtat ttgatacata
    14521 taaaaaattg gcaaatccag aagaaggata gagaaaattg agattgaaga gtttgaaaga
    14581 gccttattag atgaaaaata caaagatgta taaaaataca tattgagaaa gtatatctct
    14641 cttaaatttg ttaataattc tattatacag aaaaacatgt ttaaggggga ttttttacta
    14701 tgaaaagagt aaaattagct ataagctttt ttatattaat gattatagga atgacaatat
    14761 cttatgcaga tactagtata aatactagat acaatagatt aagcaaagct cttgaaattt
    14821 cagaagcaaa ttttaaattt tataatatta aaattaattc tgttataaat cataatttat
    14881 caaataaaga attttataat atttttatag aaataattaa caatctaaag ttggataaaa
    14941 aaggaataaa ttggaatcga aaagggtctg gcgataaaat acaattatgt gctactataa
    15001 aaggtagcga taaagaaatt tctataaaag gaataaataa aaataacaaa gaaagttaca
    15061 tagtagttga catattggat aacaaagtat ataaacataa agaggttatt tatagtaacg
    15121 tagaaaatgt gttaaataaa tattcatctc aagtagatgt gtctgcttgt attgttggag
    15181 aatacacaaa aaggttacaa aacaacaaat atgatgatat tttacaaaaa atattatata
    15241 atatgagtgc taagcaaata gatgaagtga aagacgtaaa ctttttatct ataacagcat
    15301 atagtaaagt attaaatgat tatgaattag aatatttagg ggaaaaaatt aatttaaata
    15361 taggaattaa atataatgag gacgacggaa gaacactcat atatattgcc acaccaatta
    15421 taaaattaga ttattaatta tgaggagatc aaaaaatatg gctaagataa tagtaaaaaa
    15481 gagtaatcca cttaaaggta gtgttaaaat agatggtgca aaaaatgcag ttttaccaat
    15541 aatagcagcg actttattag caaacgggaa atcaacatta aatggagtac caaatttaag
    15601 agatgttcat gttatatcag atttattaag acatgtaggg gcagaagttg aatataaaga
    15661 aaatacactt actgttgatg caagcaatat aaaaacttgt gaagctccat atgagcttgt
    15721 aagaaagatg agagcttctt ttttggttat ggggccatta cttgctagat ttaatagtac
    15781 gaaaatttct atgccaggag gatgtgctat aggaacaaga cctatagatt tacatttaaa
    15841 aggatttaaa gctttaggtg caaaaataga gatggaccat ggttttgttg aagcagcaac
    15901 agaaaaatta gtaggaaata aattatattt agattttcca tctgttggag caactgaaaa
    15961 tataatgatg gcagcttctt tggcagaagg aactacaata atagaaaatg cagcagaaga
    16021 gccagaaata gtagatttag caaacttctt gaatgaaatg ggagcagatg ttaaaggagc
    16081 aggtactaat actataaaaa taaaaggtgt taaagagcta aaaggagcag aacataatgt
    16141 aattccagac agaatagaag cagcgactta tatggttgca gcagccatga caaaaggtga
    16201 tataactgtt gaaaatgttt taatggagca tttaaaacca gttgtagcta agttgagaga
    16261 agctggttgt gaaataacag aaatggacaa ttctgtaaga gttgtaggtc caaaggtatt
    16321 aaaaccaata gatataaaaa ctttaccaca tccaggattt cctacagatg ttcaagcaca
    16381 atttatggct atgcttactg tagctaatgg aactggtgta gttatagaaa ctgtttttga
    16441 aaatagattt atgcatgttg ctgaatttaa tagaatgggt gctaatataa aaatagatgg
    16501 aagaagtgcg gttgttaatg gtgtagatga gctacatggt gcagcagtaa acgctactga
    16561 tttaagagca ggggcagccc ttattttatg tggtcttata gcagaaggag aaactcaaat
    16621 aggagaaata taccatatcc aaagaggcta tgttgatata gataagaaga taacggcatt
    16681 aggtggtcaa atcgaaatag tagaagatta ataattggtt tatagaagaa agttataaat
    16741 taatagggtt gttcatatac ttaaatacaa ggttttaaaa gctaaggagg agagtatatg
    16801 aagaacccat tgattgtttt gctaggattt gtaacttgct ctgtgttagt tccatcactt
    16861 ataactttag tttcttataa aaatgtagaa ttaactgaaa aaccagagag cccagtatct
    16921 ataaacaaaa ctattaaaaa aagtgatatt aaagacaaaa gtaataaaga agaaaagaac
    16981 atagtgaact atgaaactgt aaataaaaaa gcaccaatca tcaatgtata taatcatata
    17041 acaggaaaaa ccgaaaaaat ggacatggag aattatttat gtggagtatt agctggtgag
    17101 atgtcttcag agtttgatat agaggcttta aaagctcaat ctgtagcagc tagaacatat
    17161 gtagtatata aacaggaaca tggtaaatca agtaaacata aaaatgcagt agtatgtact
    17221 gattataaac attgtcaaga atataagagt tatgatactc ttaaaaaatt aaatggtgaa
    17281 gaatggatta agaataaata ttccaaaatc caagaagcag ttagaggaac taaaggtcaa
    17341 ataataactt ataatgataa agcaatactt cctctttatt tttctacatc ttcaggaaaa
    17401 acagaaaata gtgaagaagt attttctgca aaatatcctt atttaaaatc tgtggaaagt
    17461 ccttatgata agtattcccc taaatttgca tcaacactta aaataagtaa cacagatttt
    17521 gttaagagtc ttagaagggc atatagcact attgttatag atgtgaataa tttaagtaaa
    17581 caagtctcaa taactaaaag aagtgatgct ggaactgtgg aaaaaataaa attaggaaat
    17641 aaagaattga ctggaaaaga cattagaact gtttttaagt taaactctgc aaactttgac
    17701 ataaaatttg gagaaggata catagacttt gtagtaaaag gatatggtca tggtgttggt
    17761 atgagccagt ggggagcaga aggaatggca gaagagggct ataaatatta tgatatatta
    17821 tcacattact acacagatac aaaaataaaa gatatatact aataattgta taaaatgcca
    17881 aaaacacgtc aataatccaa atgtagaaat atatttggag gtgcaaaatg aagaaaaagc
    17941 tgttagaaaa agatggtttt tatttatctt tgtttgtatg tgtttgttta ttagcagtag
    18001 gtggagtttg gtttacaaat aataatgtag ataaattggc ttctaataaa ggaataatgg
    18061 aaaatgctaa taaagatagt gaagaggaaa tacatttaat tgaaaaagac aagaaagatg
    18121 ctatccctac tgcaactgat tcaaaacaaa acttagaaaa agctaaatca aaagaggaaa
    18181 ataaatcgag tacaacaaaa ttaaattaca ttggagataa agtaataaga ggatattcag
    18241 aaaaagaacc tagttattct aaaacacttg atgtttggga aactcataaa ggtgtagata
    18301 ttagttgtac taaaggcaaa gaagtaaaat ctctattaaa cggaatagta gtagatgtat
    18361 ttaatgatga agaatatgga caatcagtca agataaaaag tgataataac attgtagtag
    18421 tatattcaaa tctagataaa aatgttaatg ttaagaaaga acagaagata acagaaggac
    18481 aatctctagg cactgtaggt agtacttctc aaatagaaag tgaagaaggt atacatgtgc
    18541 atttagaggc atatagtgga gaaaaatcta tagacccaat gagtctaatt aagtaaaagt
    18601 ataacaagtt taatcatatt tggttaagct tgttatattt tttggtgttt ttacatatat
    18661 atcattaaag ctattatgtg aggggggtaa tctttgagat ctcatataga ggaaagggcc
    18721 atagtggtag caaaatatat acttgaaaaa aacactacag taagacaaac cgccaagaca
    18781 tttggagtaa gtaaaagtac aattcataag gatgttacag aaagattgaa ggaaataaat
    18841 ccatcccttg ccaaagaagt taaaaatgtg ctagacaaaa ataaatcaga gagacatatt
    18901 agagggggat tagcaacgaa attaaaatat gagaaagaac ataaaaaaat gtaggttaaa
    18961 aacctacttt ttttatttaa ttaaattata aaaagaaata tattaagtaa ttataaatag
    19021 caaaataata atactctcaa aagatttatt ttctcttgat taaagcgaca tttttgtata
    19081 tgctttatta taggagtatt aaaataggct tataatataa tctaatatta taaataaagg
    19141 agttgaaaga atggctggag ctgatatagg catagattta ggtactgcaa atgtgcttgt
    19201 ttatgttagt ggtaagggaa tcgttttaga agaaccatct gttgtggcga tagacaaaag
    19261 aacagattct gttttagctg tgggagaaga agctaaaaag atgataggaa gaacaccagg
    19321 taatatagtc gctataagac ctttaagaga tggtgtaatt tcagattatg atgtaactga
    19381 gaagatgtta aaatctttta tagataagat agttgataaa aagggatttg gaagattttt
    19441 tatgccaaga attatggtat gcgtaccaac aggtgtcaca gaagtggaaa aaagagcagt
    19501 agaagatgct acgagacaag ctggagctag agaagtatat ataatagaag aaccaatagc
    19561 agcagccata ggagctggtg tagacatatc acagcctaat ggtaatatga ttatagatat
    19621 tggtggaggt actgtagata tagctgtaat atctctagga ggagctgttg taagtgaatc
    19681 tataaaggtt ggtggagata gatttgatga tgctatagtt aaatatatga aaaaacaaca
    19741 caatcttctt ataggagaaa gaacagcaga gaaaatcaag tttgaaatag gttctgcatt
    19801 taagagagaa gaagaaaaat atatgaagat aacaggaaga aatttaatta caggacttcc
    19861 tagttctata actataaatt caacagaaat gttggaagca cttagagaat gtgttgagca
    19921 aatagtagtt gctactcatg ctgttttgga aaaaacgcct cctgagttag cagcagatat
    19981 aggggatgtt ggtataataa tgactggagg aggttcatta ttatatggat tagataaaat
    20041 aatagaacag agaacaggaa taactgttac aatagcagat gaaccattat cttgtgttgc
    20101 aaaaggtaca ggaatagctt taggttcaat agacctactt gaaactggtg ggtctttcaa
    20161 aagaaaataa ttaggagagt ttagaatgtt aaatatagat gaaataaaaa aattaatacc
    20221 acatagatat ccgtttttat tagtagacaa gattacagag ctagaagtgg ggaaaagggc
    20281 tgttggtata aagaatgtaa cagttaatga accatttttc caaggtcact ttccagaata
    20341 tcctttaatg ccaggagtac taatagtaga agctctagca caagtgtgtg gagtggcaat
    20401 gatgtctgta gaggaaaaca aaggaaaatt aggtgtattt gcaggtatag ataaagttag
    20461 aattaagaga gaagtaagac caggagatac attaactatg gaagttgaaa tgacaactct
    20521 tagaaaaaac atagccaagg cagatgctaa ggcatatgta ggtgaagaac ttgtctgtaa
    20581 aggtgaatta atgtttgctt tagtagaaaa ataagaactt aaaatataaa agtaaaaagg
    20641 tagataattt ggagtattta ataagatact ctaaaatatc taccttttta tataaagaat
    20701 tttatcattt agtttatatt gggattaggt ggatactaat aaagccaagg ctattgtttc
    20761 agctaaatcc aatataaagc taagacgtat attattaaat gaaagtttat tattttcatc
    20821 aattttatct acaattccta ctatagaata atttcctatt tgaggtagtt ttttgccaac
    20881 accttttccg ggcaggatag gtccttctct aatctgtata ttacctatgt tactctgcga
    20941 ccccaagcaa gcatctatag ccaagaagtt accttgtata tgtgtatttt ttatagtatc
    21001 tagagactca tatatattta aggcgtgaat agggttatct agtgtaccgt aaacggggta
    21061 tttaaagtta ctgttcttaa gtattgttcc aactaaaggg cctaaagtat cacctatagc
    21121 cctgtctgtt cctatgcaaa caacaatcgt attttcatta attatagttt ttagaactga
    21181 acttagcatc tgtataacat catcatcgct gtaatttact ttagctaaat acaataggaa
    21241 cacccccttc aaaaaaatat atgaataaaa agcattaaaa agacctaact ttaaatttca
    21301 gatagataga aacttttatt tgtcttacaa aaaaaggaat agaaaaaata tgactaaaaa
    21361 tattgacaaa attttaaaaa aatagtaagc tagtaaagta aaacaaaact taaatcttga
    21421 ttaccttatc aagagaggct gagggatagg ccctgtgaag cccagcaacc acccaaatgg
    21481 gaaaaggtgc taattcctac aagacaagag tcttgaaaga tgaggtcagt taattagtat
    21541 gtaattacga ttattaacct cttcttataa aggagaggtt ttttattttg agtaaatttc
    21601 taaaattaaa aataaggtaa aacctataat aaaaactaag aataaacaag ggggaataaa
    21661 aatggcaaga catttattta cgtcagaatc agttacagaa ggacatcctg ataagatatg
    21721 tgaccaaata tcagattcaa tattagatgc attattagaa aaagacccgc aatctagggt
    21781 agcttgtgaa actacagtta ctactggttt agttttagta gcaggagaaa taagtacatc
    21841 tgcttatgta gacattccta aattagtaag agaaacagta agagaaattg gatatacaag
    21901 agcaaaatat ggatttgatt gtgatacttg tgcagttata acttctattg atgaacaatc
    21961 tggagatata gctatgggag ttgatgaagg tctagagagt aagactggag aagagataga
    22021 agaggaaata gaaaaagttg gagctggaga ccaaggtata atgtttggat ttgcatgtaa
    22081 tgagactcca gaattaatgc cacttccgat atctttggca cataaattat caagaagact
    22141 tacagaagtt agaaagacag gtatagttga ttatttaaga ccagatggaa aaactcaagt
    22201 tactgttgaa tatgaaggaa ataaagctgt aagagtacat acagttctta tatcagctca
    22261 acattgtgaa acagtatcaa atgataaaat aagagaagat ttaattaatc atgttattaa
    22321 agaggttata ccagcagaat tacttgatga agatactaaa atttatataa atccaacagg
    22381 aagattcgtt ataggaggac cacaaggaga tacaggtctt acaggaagaa aaataataat
    22441 agatacttat ggtggatatt ctagacatgg tggaggagct ttttcaggaa aagaccctac
    22501 aaaagttgat agatctgctg cttatgccgc tagatatgtt gcaaaaaata ttgttgcatc
    22561 tgggcttgca gataaatgtg agatagaact tgcatatgct ataggtatag ctagaccatt
    22621 atctatattt atagatactt ttgggacagg taaagtttca gaagaaaaac ttgttgaatt
    22681 agtaaataaa cactttgatt taagaccggg agcgataata agagacttag atttaagaaa
    22741 gcctctttat aaaaaagttg ctgcttatgg tcactttggt agaactgata tagatttgcc
    22801 ttgggaaaga actgacaaag tagagcagtt aagaaaagat gctttaggtg aataattttt
    22861 aactaaaaag ctgttagaat gtaattttac aattactttc taacagcttt ttattatgag
    22921 ttaaatattt ttaataaatg ctaatttatc atcatataat acataaacag tatttaatct
    22981 ggtcaataca ttttttataa taatctacta aatttataaa tattatgaga tatatgttaa
    23041 aggatggtta agaatgtgtt aactaatgtt aaataattaa aaatatattg aaatatcata
    23101 ataatgagtc taatataatc ttaataaact tacactttaa aataatttta attgggggga
    23161 gtagtgtatg tcaggtaaag taaatattaa aacggttata agttttgctg gagcttatgt
    23221 tgctactgta ataggttctg gatttgcaac tggtcaagaa atactacagt tttttacgtt
    23281 ttatggatat gctggtattg taggtggaat aatatctatg gtactatttt catggtttgg
    23341 agcagaagtt atagataagg gaagagaatt aaaacttaag gagccaataa aaatttatca
    23401 agtgtattgt ggaaaatacc ttggaatatt ttttgaatgg tttggtccat tgtttttatt
    23461 tggaacatta gtaataatga ttgcaggagc aggagctaca ttatcagagt actatggatt
    23521 aaatccatat gtaggtagga ttggtatggc aatcgtttcc ctaataactg tttcattagg
    23581 tttaacaaga ctttctaaaa tactaggtaa tataggccct ataataataa tatttacact
    23641 tttggtagga gcaattagtt tatttagcaa tattgatgct ttatcaaatg ctggaaatat
    23701 ggttgatagc ttaaatatta aaactgctac atcaaatgga tacttttcag gagtacttta
    23761 tacttgttat aatgtaataa tagtaataac attcttgaca ggaatgggag cttctgcagt
    23821 aaataagaaa gatgctgttt ggggtggtat agtaggtgga gtagcactta tggcagctgc
    23881 tataatgatg aatttggcac tactttcaga cataggaaac atatacacaa aagagatacc
    23941 agcactttac ttagctgata agatatctcc aataataggt attttattct cagtagtact
    24001 tttactagga atatacacta cagctgtacc attgttatgg tcagtaacta acagatttgt
    24061 agaagatgac caccctaaat ttaaaattat aacaatagta gtaagtatat tagcttgtat
    24121 aggaggtcta ctaccatttg ataagttagt aggtacacta tatccatata caggttacat
    24181 gggaatttta attttactat gtattcttta tagaagaatt actaaaaccg agggatacaa
    24241 ggagaataaa tctgaaataa gttagcactg taatattctt atgaaattta atttatactc
    24301 taaacataga gtaattaaaa taaaatagat atttaaatat ggtacctgta tttcatggta
    24361 ccatatttta tggatgcaga ttttttttgt ttctgataaa atgtataata gttggaaatt
    24421 acgttaagta taaaaatata aaacttttct aagattatca tagttttaat atataaaatt
    24481 atactaacat ttaaatggag cgattattag tatttttagg agataaaaat ggaaaaatta
    24541 gaaggaatga taagtgaaat agtctttaaa aacgaagata atggctatac aatagctcat
    24601 ctggtaaatg aaaatgatga gattgtcgtt gtaggatgta tgcctacttt ggcaattgga
    24661 gaaagcatag aagttgaagg aaagtgggta aaccataagg tttatgggac tcagtttgaa
    24721 gtgaatagtt ttatgccagt aacaccatca tccttagaag gaatttatgt ttacttatct
    24781 tctggaatga tacatggtat tggtgaaaaa atggctaaga gaataattga taagtttggt
    24841 gtagatacgt tagaggtgat acaaaattca ccagaaaaac tacaagaagt agaaggtata
    24901 gggagtaaaa aagtaaaaca aatagtaaaa agttatgaag aagacagaga attgagaaat
    24961 ataataatac agctatctcc ttttggaata acaccaaatt actgcctaaa aatatataaa
    25021 aaatataaaa gcagtgcaat agatgttata aataaaaacc cataccaact tgctgaagat
    25081 ataagaggaa ttggatttaa agtagctgat agtatagcaa ataaaattgg tatagataaa
    25141 aactctaagg atagaatttg tcagggtatc ctatacactt taaataaatc tttaagtaat
    25201 gggcatacat atttaccaga gcatgttcta atacaagatt cggaaaaatt attggagcta
    25261 agtgggaaaa taataaaaga gtgtgtaatg atgctagttt ataatcaaaa aatacatata
    25321 gagaaagtaa acaatgaaaa tctcatatat cttatgccat actatctagc tgaaaatgga
    25381 gtatgtagtc aaatagtaaa attatcacag tatgaatttg aagacttaaa aatagatata
    25441 gataacgaga ttaatgtttt agaagaaaat aagaaaatta agttagcaga aaaacaaatt
    25501 ttagcagtca aagaatctgt taatagtgga gtattaatta taactggtgg gccaggaact
    25561 ggtaaaacaa ctactatcaa tgctattata gacatatttg aaaacaatgg taaaagcgtg
    25621 actttggcag ctccaacggg aagagcagca aagagaatga gtgaaacttc taataaagaa
    25681 gcaaaaacta tacatagatt gctagaaatg ggattctcaa cagatgatga tttaactttt
    25741 tttaaggatg aagaagaccc aataaattca gatgtaataa ttgttgatga ggtatcaatg
    25801 gtagacatta ttttaatgta caacttgctt agagctataa agttaggaac aagagtaatt
    25861 ttagttggag atagtgacca attaccctct gtaggtgctg gaaatgtact taaggacatg
    25921 attagttcta atattataaa tgtagttaaa ttaaacgaaa tatttagaca agcgcaagaa
    25981 agtatgataa ttgtaaatgc ccataagata aataatggag aaccacttta tctaaataca
    26041 aaaggtaaag atttcttttt tataagaaaa tcaactaatg aagaaatttt aaatgaaata
    26101 ataggattgg ttaatgaaag attacctaaa ttttataaag ttgataaatt aaaagatata
    26161 caagttttat cttcaatgag aaaaggtgaa ttaggagtta ctaatctcaa tatagagtta
    26221 cagaagtatc ttaacaaaaa agaaaaattt aaagttgaag aaagtttttc taaaagatta
    26281 tttagagttg gcgataaggt aatgcaagta aaaaataatt atacaaaaaa atgggaaaca
    26341 gaagaccaaa aagaaagtgg agaaggtatc tataatggag atataggata tgtttatcac
    26401 atagataaag ataagaaaac tatttatgta ttatttgacc agactaaaat tgtatcatat
    26461 ctatatgatg aattagatga aattgaccat agtttttgta caacgataca taaaagtcag
    26521 ggaagtgaat ttccagttgt agtattgcca ataacttggg ctcctcctat gctacttagc
    26581 cgtaatttat tatatacagc agtaacaaga gcaaagaaat tagttgtatt agttggagat
    26641 gtgaagtatc ttgaatatat gataaaaaat aatagagtta atcaaaggta ttcaaattta
    26701 gggtataagc taaataaatt taagcaagaa gggttgttga tagaataaat atgaaaatat
    26761 gcaatgataa ttttattaag gattttaaaa atatgattaa taaatgtctg gattttatat
    26821 atcctgagaa tataagttgc ataatttgtg ataaatccat aaagaaaaca aatacttatt
    26881 caatatgtaa aagttgtttt aaagaaatga attttataca agatggttgc atgaagtgtg
    26941 gaaaaccaat aatacatcat tcaattgaaa aagagtttat tgaagagtgc agttattgtt
    27001 ttaacaaaga cttttacttt gataaatcaa tatcttgtat tgaatataat gatgtaacaa
    27061 aaaagatagt gttagggctg aagtataacc aaaaaacctt tatggctaag tatatagctc
    27121 aaataatgaa agaaaaatta tatttagaaa acataaaatt tgattatgta ctatttgttc
    27181 cacttcataa aaaaaggtta aataaaagag gttttaatca ggcgcaaaaa atagcattta
    27241 atttaagtaa aatagtaaat attccattat tggattgtat aagtagaaaa aaatatacaa
    27301 gaatgctata taaattaaat aaaaaagaac gaaaagaaga gttaaaaaat gtatttgttg
    27361 ttaaagaaaa tattaaatta ataaacaata aaaatatact tttaatagat gatatattta
    27421 ctactggtct aacaacaaat gaaatctcta aacttttaaa gttatccggt gctaataagg
    27481 tttttgtatt aactctactt acaaaagcaa atgataatta tattatggaa taatcttata
    27541 tattgacaat tttaatttaa aatattaaaa tgaataggtg atatttaggt ctgtggttga
    27601 aagtccaagc cagtcgcagg caaaaggatc cacgtaagtt aacactaaaa attgggttaa
    27661 tgatcatggt gcggcttaga agtaagacct accgataata aaatcgagag agagtagtag
    27721 tgcggcgaac ccaagcgaac cctccagtag gcgagtgtgg ggtcaaaaac caggtcagct
    27781 aaatatcata ttaaaagaga gctagcatac ctttatgtat agctcttttt tatatataat
    27841 aaattaaggt tgagtcataa agaggtgata tgtttgaata aaagacaaga aaatttatta
    27901 acaacattgc tattaaaaga agagtttata aatgttaatg aaattgccaa tgattttgag
    27961 tgttcagaaa ggacgataag aaatgattgc aaatatattg atgaatggct atctactttt
    28021 tgtaatgctt gtatcaaaag aaaacctaat attggaatta aatttagtgg agatggaaca
    28081 gataaaatac tggtaaatca gaagttgcga ggtgaagtta aaagagtaaa tagtgagctt
    28141 cataagcaag ttgagatatt aaatttacta ctttgtaata ataaaaaagt tactttaaat
    28201 gatttgtcta ataagttgta cataaataaa aatattgtca gagaagaaat aggtaaatta
    28261 tcagtggcat taaataatta taacctaaat atctcaacta aaaaaggtag tggtatatat
    28321 attgaagggg atgagaaaga cataagaata atgttagtat cattcttatt taaatacata
    28381 gataaataca gattaaacat agatgaaata gaatatttcc atatagtaga tataataatt
    28441 gtaaaaaaca taatgtttac tatagaagag aatatggata taagacttac agatatatca
    28501 ttcaagcaat tgatgctatt tttgcttata aatataatac gaataaggct aggaaattct
    28561 ttaaaagtat caaatactaa gaaagagctt aagctaaaaa tgcttatact agatatagaa
    28621 gatgaactaa aatctaattt atctatagta ttaaattatg aagaaaagct gtttatagaa
    28681 tctttaatat tgggtatgaa taaacaaata aatagcagta atattaagaa atctttatat
    28741 ctgaatgaag aattagcaaa ttatacaaga aaaattataa atttagtatc agaagaatca
    28801 ggaataaatt tcaataatga tatattactt tatgaacaat taatatgtca tctgaatgtt
    28861 acaatgcatc aaatgaaaag caatgtttac ttagaaaatc cacttttaga taacataaaa
    28921 actaaatttt gttttttatt tacagtaata tcaagttcaa tagaaattga attaaaagga
    28981 aaacaaataa cggaagatga aatcggttat cttacacttc actttcaaac ctctctagaa
    29041 agaaaataca gtaaaaggga atcaaataaa aaagcaagta ttgtatgtcc atttagtttt
    29101 ggagtaagta tgctattaaa agtaaagata gaaaagagat ttgacaacat aaaaattatt
    29161 gaaacattaa gagaggaaga tttaaaaaga gataacttca ataagacaat agattttata
    29221 attgcatttc aagagtatga gaatattcaa aaaccaattt ttataacaac accattattt
    29281 actgatgagg atgagaataa acttaaagag ttcacaaaca aaataaaaga aaaagatact
    29341 tcatataagc ttatgaacag attgatgatg tctgattatt taataagaga gttagaacat
    29401 gatgatattt atgaagctat aacatattta actaatttat tgattgaaaa acattatgca
    29461 gaaaaagagt atttacaatc tattataaca agagaaaagt catacccaac caatgttggg
    29521 aaaggaatat tattccctca tggagatatg aaatatataa aacaatctgt tttatgtttt
    29581 gcaaggttaa aaacaccaat aaaaattaga aatggaaaag atataaagat tatactttta
    29641 ctagcctata aaaaagatga tgatagaaaa gtatttagag aattatttaa agaaatatct
    29701 aatttaactg aggatgaaaa tatgctagat attctaagta aatgtgacat tgaaaaagta
    29761 aaagatattc ttatataaaa ttcatagaga cctatggata agcacaaaat tttccatctg
    29821 atggaaaaag ttgtgcatgt tttttgtttt ttataagata ttatatgttt ataaggtata
    29881 aataatctaa aaacagagaa gcaatttagg aggaaaaaat ggaagaaata gtcttaaaga
    29941 ttataattca cgctggaaat gcaaaatcta tgctttatga agcgcttgat tatgcgaagg
    30001 aaaacgattt taaaaaagcg gatgaattaa ttgaaaatgc aaatgaagaa atcttaaaag
    30061 cacataaagt acaaactgaa cttatacaaa aggaagctgg gggagataaa tcagatatat
    30121 ctattttatt aattcactct caagaccatt taatgacttg tatgagtgag agaaatctta
    30181 taaaagaaat gattatgtta agaaaagaaa ttcaaaaatt aaaatgaaaa atttaagggg
    30241 gatttaaaag aatgaaaaga aaagtatatt tattttgtag ctttggtatg tcaacaagtt
    30301 tacttgcaga gaaaatgcag aaggtggcag atgaacacaa tttaccaata gaagtagagg
    30361 catttccaat agcagaaatt gataaaatag ttgaagaaaa aaatccagat tgtatattat
    30421 taggaccaca agttaaatac atgctaaagg aattaaaacc aaagtttgag gctcaaggta
    30481 aacttataga tataataaat gaagttgatt atggaactat gaatggggaa aaagtactaa
    30541 agcttgctat aaagttaata aaatctaagc aagcatagga ttataaataa aaaacaatga
    30601 gagatgaggg tggatagatg aatagattgg aaagaatact tatgccattg gctgaaaaaa
    30661 taggtaaaaa taagttatta attgcaatta gagatggttt tttaatatca tcaccattat
    30721 taataatagg ttcattattt ttattagtat caaacttccc aataaaagga tggaatgaat
    30781 ttgtaggtca aatttttggt ccagattggg ctgtaaagtt acaacaacca actgtagcaa
    30841 catttgaagt aatgactcta cttgcagttt taggaattgg atattcttat gcaaagcaaa
    30901 tagatgtaga cccaatagca tcagcagcag ttgcaatagt tgcctttttt ataataacac
    30961 catttgtaat accatataca ccagagggaa cagaatcagt atacatggtg acaggtatac
    31021 cactaggttg gatgggttct aagggaatgt ttgtaggtat gataacagca ataacctctg
    31081 taaaattatt tgctgcagtt gtaaaaaaag gatggacatt gaaaatgcca gatggagtcc
    31141 ctccaactgt atcaaaatca tttgcagcat taataccaag tgcaatagtt atggtagttt
    31201 tctttttagt taagatagct tttgaagcta caccatatgg aagtgtacat gaatttatat
    31261 ttaagttttt acaaatacca ttattaaaat taggaaatac tttaagtgct acagcaattg
    31321 cttatgtatt cttacatcta ttctggttct ttggaataaa tggaagtagt gttgttggag
    31381 ctgtgtataa tccaatatta aaaattctat cagctgaaaa cttagctgct tttcaagctg
    31441 gtacaaaact accaaacata ataactgctc aatttcaaga tatgtttgca acatttggtg
    31501 gagcaggtag tacattatca ttaattatag ccatgttatt aatatgtaaa agtaaacgta
    31561 taaaatcaat aggaaaatta tcaattttac caggtatatt tggtataaat gaaccattaa
    31621 tatttggtct accaataatg cttaatccaa tactattgat accatttgca ctagttccaa
    31681 cagtaaatat aataatagct tatttctgta tgtcaacagg attagtgcca cttacaaatg
    31741 gagttcagct accatggaca actccaatta tattctctgg atttctaaca acgggatggc
    31801 aaggggcagt tttacaatta gtacttatta tactaggaat atttatgtat ataccattca
    31861 taaaaatgct agataaacaa tatttgaaag acgaaaaatc aaatgttgaa ggggaagatg
    31921 atgatatatc ttttgatgat ttagtattat aggaggaaga ttatgaagaa aatagtaatg
    31981 atattggatc aaatacaagc tggagcggga ggaaaagaga aaagtaatat acctcctgct
    32041 ggaaaatcaa gcccattagg accaggtgta atgatggaac catttttaaa tgaatctaaa
    32101 gtaatagcta cattattttg cggggatgaa ttttttataa ataatgaaga agaagtgact
    32161 agtaagatga tagctatggt aaaaaaatta aaccctgatg tagtaatctg tgggccaagc
    32221 tttaattatg aaaacttttc taagatgtca gcaatactgt ctaaaaatat aaacgataag
    32281 gcagatatac cagcttttgc agctatgtca gaggaaaata tagatgtgat aaatgaatat
    32341 aaaaatgaca tatgtatagt tagaactcca aaaaaaggtg gaattggtct taatgattcg
    32401 ttaaataaca tatgtaaatt agctaaagca atggcaaata aagaagatat aactttgatg
    32461 aaagaagaat tttgttatta atatgagtat taattattag caagtttgaa tattacacta
    32521 tttataataa gcatatatta ttaaaatcta tatagtttaa ggaggggaat tttagcatgt
    32581 ggggaatgat tgcgacatgg agaatggccc tagaaggaat aactgaggca tcagaacatt
    32641 tagaaaaagg catggaagca ggtgatgctg tagagcttgc tgtaagaaga gttgaagact
    32701 atccattcta taaatcagtt ggatatggtg gacttccaaa tgaaaattgt gaggtagaac
    32761 ttgatgctgc atttatggat ggtaacacat tagatattgg agcagttgct ggtataagag
    32821 attattctaa tcctgtcagt atagctagga agttaagtta tgagaaggta aataattttt
    32881 tagttggaat aggtgctgaa gagtacgctc ataaaaatgg atttgaaaga aaaaatatgt
    32941 tgactgatag agcaaagtta cattataaga aaagaaaaaa agagacttta gataagggat
    33001 taagtccata tgctggtcat gatactgttg gtatgatatc tttagataaa actggaaaaa
    33061 tgtgtgcagc tacatcaaca agtggattgt ttatgaagaa aagaggtaga ataggagatt
    33121 ctgcactgtc tggttcggga ttctatgttg atagtagtat aggaggagct acagcgactg
    33181 gtttgggaga agatttaatg aagggatgta tatcttatga gatagttaga cttatgaaag
    33241 aaggaaaaca tccacaagaa gcatgtgata ttgcagtaaa cgaacttgat aaacagctta
    33301 ttaaaagaag aggaaaagct ggtgatttgt cattagttgc aatgaataat aaaggtgaat
    33361 ggggagtagc tacaaatata gctgatttta ctttttcagt agtaactaaa gaagaatccc
    33421 ctactgtata tatttcaaat agagtagatg gaaagactat acataaggtt atatcaaatg
    33481 agtggatgga agcatatgag gctagaataa aagcaccaat agaagtaagt gagtaaagta
    33541 gtagttacat aattaaaata gagttttata ataaggagat ttttatgaat atcataagta
    33601 ataaaataga tgaattaaaa gaagatttat taagtgatat tatagatatt gtaaaaatac
    33661 ctagtgtcaa aggggagtca gaaaatggct tcccttttgg tgaaaaagta ggggaggcac
    33721 ttaataaagc attagaaata tcagaaaaat tgggatttaa gattagaaat ttgaataatt
    33781 atattggtta tgcagagcat ggagatagcg aggattatgt ttgtgtaatt ggacatgtag
    33841 atgttgtaca tgaaggagac ggttggaaat atcagcctta taagggagaa gaaatcaatg
    33901 gaagaattta tggaagagga gtactagata ataaaggtcc tataatatct gctttatatg
    33961 gattatatgc tattaaagaa ttaaatttaa aattagataa aagagttaga ataatatttg
    34021 gtacaaatga agaaagtgga tttgaagata taccatatta tttagaaaaa gaaaaagcac
    34081 ctatcatggg gtttacacca gattgtaagt atcctgttgt atatggtgaa aaaggcatgg
    34141 caaagattag aataaaaagt aaaataaatt atgaagaaga tgtattatta ggttttatag
    34201 aaaatgtgtc tgaaaatgtg cttgttacat ataaagagct aaatatagaa aatagtgata
    34261 ctatattgga tataaaggta aaatacgatt ttagttataa attaaaagat gtattagatg
    34321 aaataaaatt tagttttcca aatagtatcg atatagaggt aatttctaat tttaatcctg
    34381 tatattttga taaagagagc aatcttgtta aaaagcttca gctggcttat gaaagggtta
    34441 catcactaga tggaactcca gtaacaacta atggaggtac atatgcaaaa gtaatgccaa
    34501 atatagttcc gtttggccct tcattcccag gacaaaaagg aatagctcat aatcctgatg
    34561 aatatatgga tatagaagat ataatattaa atgcaaagat atttgcaaat gcaatatatg
    34621 aattagcaaa ggagtagtta tgttagagat aataggaatg tcagtagaag acgctaaaat
    34681 tatagaagat tgtggtgcag atagaataga gttagttagt gctttaactg aaggtggatt
    34741 gacaccaagt tttggtctta tagaaagggt tgttaacagt gtaaagatac ctgtgaacgt
    34801 aatgataagg cctcatgcga aaagttttgt atacagtaaa gaagatataa atataatgca
    34861 aaaagatgta agtgttgtta aagagattgg tgctaatgga gttgtatttg gtgtattgga
    34921 taaaaacaac aatatagatg agaaaaatct aaatgtttta ttgaaatgtt gtgataatct
    34981 tgatgttaca tttcatagag ctatagatga atcaaataca atagatagtg ttaaaacttt
    35041 gaaggattat gataaaatta taaatatatt aacatctggg ggaaaaggaa gtatagttga
    35101 taacatacaa ataataaaga atatgatgct tagttctaat cacataaaaa ttcttttagg
    35161 tggaggactt aattttaata atatagaaaa gataaaggaa ttaactaagg caagtaattt
    35221 tcattttggt acagctatta gaatgaataa cagtcctttt gaagatatag atagacaaaa
    35281 gcttagacag cttgtaaata taatttcaag atagtatata aatataaaat acttagttta
    35341 aataaagaga gtaagttaat tataatttgg gtatatgaat gttaataggt attacatatg
    35401 aaattattgt tgagtatgct ttacaacttt taagtagttt taaatataaa taatgaaagc
    35461 aaagatatag taacgaggtt ttagagggtt tatatttatt aaaaaattat aaaggtatac
    35521 tggcatataa cacatataat ttcctaaaat taccatctta tgacataaat tcacatatta
    35581 ataaacaatt aatggagatt tttgtggata gaaataggag caaaaatagc tttaaataaa
    35641 caaaaacagg agttattaac agttttatcc acattatcca caggtgtttg taataatatt
    35701 aatccacata agtataaatt taactaaaaa tgataaataa ttctatcaat tgaatagaaa
    35761 aaatcataaa agtaaataat aaaaagagga ttatgagagt tcgcgtagaa tatattatta
    35821 aagtataaat tttcttatac tggcataatt gaagggagat gtacttatga acataattat
    35881 atctggaaaa caaatcgaat taactgatgc aataaaaaat aaaatcgaaa gtaaactaag
    35941 taagttagat aattacattc atccagacac tgatgtaaaa gttacagtaa gtgcgagaaa
    36001 agcaagacaa aaaatagaag taacaatagc tactgtaaat ggacctataa ttagagcaga
    36061 agatatgcag gaaaatttat acacagctat agatgttgta tatgataaat taagtaaaca
    36121 attgaaaaaa tataaaaaga gactacaaga taagcataaa gacaacaaga gtataagatt
    36181 ccaagacgca gagataaatc tagctgatga acaagaggat tatggggatg aaaatcaaga
    36241 attagtaatt caaagacgta agaaatttag tgttaagcca atgagtgaag aagaagctgt
    36301 attacagatg gaactaatag gacatgattt ttatatgttc aagaacatag attcagatga
    36361 aatagcagta gtttataagc gtaataatgg tggatatggt ataatagaac atgaataaag
    36421 caatttagat ttaatcatat tagctatcga taaataaatt atcgatagct ttttaaatta
    36481 atattaaaag tatagttttt atggtaaaat gaataatagt acaataaaga acgaggagag
    36541 atgtaaaata tgagctttat ggataattta ttcaacatgg cagataaaaa agaattaaaa
    36601 aagttcaata agactgttga tataatagat tcattagaac ctaaatttga atctatggca
    36661 gactcggaac ttaaaaatat gacaaatata tttaaagaaa gattagcaaa tggtgaaagt
    36721 atagatgata tacttccaga agcttttgca gtagttagag aagtttctaa gagagtgtta
    36781 ggacttagac actacagagt tcaaatgatt ggaggtatag tactacatca aggaagaata
    36841 gcagaaatga aaacaggtga aggtaaaact ttggttgcta ctgctcctgt ttatttgaat
    36901 gcgcttactg gaaaaggtgt acatgttgta acagtaaatg attaccttgc aaaacgtgat
    36961 agagaccaaa tggcaaaaat ctatgagttt ttaggaatga gtgtaggcgt tattatacat
    37021 ggtcaaaacc caaaagtcag aaaagaacaa tatgattgtg atataactta tggtacaaat
    37081 aatgagtatg gatttgacta tttaaaggac aatatggtaa tacacaaaga gcaaagagtt
    37141 caaagaggat taaattatgc tatagtggat gaggttgact ctatactaat agatgaggct
    37201 agaacaccac ttatcatatc tggacctgga gacaaatcaa ctcacttata ttcggatgca
    37261 aacacatttg ttttaacatt aaaaccagat gattatgaat tagaagaaaa agataaggct
    37321 gtatctctaa cagcatcggg tatacaaaaa gcagaagtat attttaatgt tgataatata
    37381 acagatatat ctcatacaga attataccat catataaatc aagctttaag agcacatgta
    37441 ataatgaaga aagatgttga ttatgttgct aaagatggag aaatagtaat agtagatgag
    37501 ttcacaggta gacttatgtt tggtagaaga tattcagaag gtttacatca agcaatagaa
    37561 gctaaagaag gtcttaaaat acaaagagag tctaaaacac tagctactgt aacattccaa
    37621 aattacttta gaatgtacaa aaaactttct ggtatgactg gtacagcaaa aacagaagaa
    37681 gaagagttta aagcaatcta caaaatggat gttttccaag tacctactaa taagcttatg
    37741 ataagggaag acttgccaga ttgtgtatac aaaagtgaaa taggtaaatt caatgctgta
    37801 gcccaagaaa taattgaaag gcataaggtc aatcaaccaa tacttgtagg tacagtatca
    37861 atagaaaaat cagaattatt gtcacaaata ttaaagaaaa agggcataaa acatgaagta
    37921 ttaaatgcta agcatcatga taaagaggca gaaataatag cacaggctgg tagacttgga
    37981 gctgttacaa tagctactaa tatggctggt cgtggtacag atatagttct aggtggaaat
    38041 ccagactttt taactaagag agaaatgaga agaaatggat ttaaagaaga aatagtaaat
    38101 agagtagata ctccaataga aggtatacct gtaaaaggta atgaaatttt atttgaagct
    38161 agagaagagt atgaaaaact atttgaaaag tttaagcaac aaacgcaaga agaacaaaaa
    38221 caagttgtag aagcaggtgg acttgctata attggtacag aaagacacga atctcgaaga
    38281 atagataacc agttgagagg tcgtgctgga cgtcaaggtg acccaggtag ttcaagattc
    38341 tatataggtc ttgatgatga ccttatgaga ttatttggta gtgatagaat atctggtatt
    38401 gtagataaga taggtcttga agaagacatg cctatagaac atagaatatt atctaagtct
    38461 attgaaggag ctcaaaagaa agtcgaaggt aaaaactttg gaataagaaa gcatgtactt
    38521 caatatgatg atgttatgaa taaacaaaga gaaataatat acgcagagag aaaacgtgta
    38581 ttagaaggcg aagatttaca agaacaaata caatcaatga ctcattctat aatagaagaa
    38641 gcagttactc tttatacaca agataaagga tttgatgaag aaggattcaa agaacatatg
    38701 tataatctgt tcttgccaaa aggtagcata gaaataccag aaatagaaaa acttaatcca
    38761 gttgaaataa cagaaaaagt ttatgaaata gctatgaaga tatatacttc aaaagaagaa
    38821 caagttggct atgagagaat gagagaagtt gagagagtaa tcttacttca agctgttgat
    38881 aaccattgga tagaccatat agatgctatg gaccaattac gccaaggtat cggtcttcgt
    38941 gctgtaggtc agcaagaccc agttattgct tataaaatgg aaggttttga tatgtttgat
    39001 gaaatgaata aacatatcaa agaagatact gtaagatatt tatttaatat aacaatagaa
    39061 actccagtgg aaagaaaagc agttgtagat gttgaaaatc tatcttctcc atcagatgga
    39121 acacttccaa taagtaaaac tgttaaaaaa gatgaaaaag ttggaagaaa tgacttatgt
    39181 ccttgtggaa gtggtaaaaa atataaaaac tgttgtggaa gataatcttg gaggattaaa
    39241 tgttaaagtt acaagaatat agacatagta tggagcagat ggcttcaaat ttagaagaat
    39301 tgagggtttc tctttgacat agcttcattg atgaaaaata tacaaattaa tgaagaaaag
    39361 atgaataaac aagacttttg gaatgataat gaagtcgctc aaagagtact tcaagaaaat
    39421 aagtccttaa aagagacatt agaagaatat gaaagcttaa aaagcttatt agaagatata
    39481 gaagtattaa ttgaaatagg tctagaagaa gaggatgatt ctgtagaaag agatatagaa
    39541 aagtccatag aatcaatgga agaaaaatta tccgaaatga aaataaagac tcttctaaat
    39601 ggagagtatg ataaaaataa tgctatatta tctataaatg cgggtactgg tggattagat
    39661 gctcaagatt gggctcaaat gcttttgaga atgtatataa gatggtcaga aagtaagggt
    39721 tataaagtta agctcttaga tataatatca gaccctgaag cagggataaa gacagctaca
    39781 attcttgtag aagggacaaa tgcttatggg tatttaaaga gtgaaaaagg agttcatagg
    39841 ttagtaagaa tatctccatt tgacccatca ggaaaaagac atacttcatt tgcatctata
    39901 gatgtaacac cagagttaga tgaaaatata gaagtagaga ttaatccatc agatttaaag
    39961 atagacacgt atagagcttc tggagctgga ggacaacatg taaatacgac tgattcagca
    40021 gttagaataa cacatattcc tacaggtgta gtagttcagt gccaaaatga aaggtctcag
    40081 catctaaata aggatagagc tatgagactt ttaatggcaa agttgataga attaaaagag
    40141 ttagaacaaa aggaaaaaat agaagatata caaggcaaat actcacaaat aacttggggt
    40201 agtcaaatta ggtcatatgt ttttcaacca tataaattgg ttaaagacca tagaacaaat
    40261 gctgagtttg gaaatgtaga tagtgtaatg aatgggaata tagacttgtt tattaatgaa
    40321 tacttaaaaa tgaataaaat agtttaataa atatgcattg taattaagat atatgtataa
    40381 gaaggttagt gcttaatatg cacttacagc acttaggctg aggacgataa ccctcagcct
    40441 tcgttctgtt ttgttatact tatgaaaaaa ctaacattag gagtgaataa attggatata
    40501 aatcaaatct taaaaaaaga gtttaatctt agagatgaac aaataaataa tacattaaaa
    40561 cttattgatg aagggaatac aataccattt atagctagat acagaaaaga aatgactggt
    40621 gaaatgagtg atgttacact aagagaattt tatgaaaaat tgatgtattt gagaaatctt
    40681 caaagtagaa aagatgatgt agttaggcta atagatgaac aaggtaaatt aacagatgaa
    40741 ataacacaaa atatagaaaa agcaaaaaca ttgcaagaag tagaagatat atatgctcca
    40801 tataaacaaa agaaaagaac tagagcaaca atagcaaaag aaaaaggatt agaaaactta
    40861 gcattgtcaa tactagagaa taatttagat aatattgaaa tagaggcaga gaattatctt
    40921 gatgaagaga aagaagttct ttctattgaa gatgctctaa aaggtgctag agatataatt
    40981 gcagaattag tatcagatga tgctaagatt agaaagtata taagagaatt agcattaaga
    41041 gaaggtatga tagttagtaa aagtgccact gatgagaaat cggtttatga tatgtattat
    41101 gattatagtg aagcagtaaa aagtatggct ccacatagag ttttggcaat aaacagaggt
    41161 gaaaaagaaa atttcttaaa ggttaagtta gaaataaata atgataaggt tttaaattac
    41221 ataataaatg aatatgtaaa tgataaaaat ttcaaaaata aagaagaaat tgtaaattca
    41281 atagaagatt cctataaaag attaattttt ccatcaatag aaagggaaat aagaaatcat
    41341 ttaacagaaa tagctcaaga aagagctata agtgtgtttg gaaagaatgt taaaagttta
    41401 ttacttcaac caccagttaa agacaaagta gttatgggat ttgaccctgc atttagaact
    41461 ggatgtaaaa tagctgtagt agataaaaat ggtaaactac tagactatac aacagtatat
    41521 ccaacagacc ctcaaaatga tgtagaagga gcaaaaaaag ttttaaaagg attgattgaa
    41581 aaatacgata tagatataat ttcaattgga aatggtactg catctagaga atcagaaact
    41641 tttgtatcag aaatgataaa agagatagat agagaagtgc agtatgtgat agttagtgaa
    41701 gccggagcat ctgtttattc agcatctgaa cttgcaaatg aagaacatcc agatataaat
    41761 gtatcaataa gaggagctat atctatagct agaagattac aagacccatt ggcagagctt
    41821 gtaaaaatag accctaaaag tattggtgtg ggtcaatatc agcatgattt aaataaaaag
    41881 agattagaag aagtactaga tggagttgtt gaagattctg taaacagtgt tggagtagat
    41941 ttaaatacag catcatactc tctactagag catattgcag gaataagtaa agctattgca
    42001 aaaaatataa tagcatatag agaagaaaat ggagatttta catcaagagc acaattgaaa
    42061 aaagtaaaaa gactaggacc tcaagcattt actcaatgtg ctggatttat gagaatctta
    42121 gagggtaaaa atccattaga taatacaggt gtgcatcctg aaagttatga tatatgtaaa
    42181 aaaatgattg agataatcgg gtattcttta gatgatgtaa agaataaaaa tatagatgaa
    42241 atagatgaaa aaataaaaga aattggcttg aaagaattaa gtgaaaaact agaagtaggt
    42301 caagttacat taaaagatat catagctgaa atcaagaagc caggaagaga ccctagagaa
    42361 gaaggtataa aacctatact tagaactgat gtcttaaaaa ttgaagatat acaagagggt
    42421 atgattttaa aaggtacaat aagaaatgtt gtagattttg gtgcatttgt agatattgga
    42481 ataaaaaatg acggattagt tcataaatca gaaatgagta atagttttgt aaaagaccct
    42541 atgagtattg ttacagtagg tgatatagta gatgtaaaag tcattggaat tgacttaaac
    42601 aaaaaaagag ttgccttatc aatgaaaaaa tagataagtt tatgtatggt tatagtactt
    42661 aaattctatg tttgtaaatg aagttaccag agtgtataaa tgttaaaaac ttgtaaaggt
    42721 atatagtaag aaaatacatt aaaataagaa cgatagtctt gaaaatcagg aggaaaatga
    42781 atgattttta taaaaaatgg taaaataaat accataacaa atgggataat tcatggagat
    42841 atacttatag aggaaggaaa aataatagaa atcggagaag atttgatagc acctttagat
    42901 gtagaggtca tagatgcaag taataaactc gtgtttcctg gatttataga tgctcataca
    42961 catttaggtt tatgggaaga tggtataggg tttgaaggag cagacggaaa tgaagagact
    43021 gacccaataa caccacaatt aaatcctgta gatggaatta atccaatgga taggactttt
    43081 aaagaagcat ttgaaggtgg aataacttct gtttgtacaa ctcctggaag tgcaaatgtt
    43141 atgggtggtc aatgtatagc cataaaaaca tatggaaaaa gaattgataa aatggtaata
    43201 aaaaatccag ttgcgtctaa aattgctttt ggggaaaatc caaagagctg ttacggtcag
    43261 gatgataaat ctccccaaac tagaatggca atagctgcct tacttagaga aaacctaaaa
    43321 aaagcagaag aatatttgga agatattgat atgtatgaga gtcatgatga tgaagactgt
    43381 gaaaaacctg aatatgatat aaaaatggaa agtttaatac ctgttttaag aagagaaatt
    43441 ccttttaagg cacatgctca tagagcagat gatatgttca cagcaataag aattgcaaaa
    43501 gaatttaatt taaagcttac attagaccat tgtacagaag gtcatcttat tgcagatgaa
    43561 ttagtggaag aagggtttcc tgtaatagta ggtccatctt tgtcagaaag gtctaaattt
    43621 gagcttagaa atttgacatt taatacagca ggaatacttt caaatgcagg tcttgatgta
    43681 tgtataatga cagaccatcc agtaatacca gttcaatatc ttccaatatg tgcaggtatt
    43741 gcagtcaaac atggtatgaa agaagaaaaa gcaatagaat caattacgat aaatccagct
    43801 aagactttag gaatagaaga tagagtaggc tctatagaag ttggtaaaga tgcagaccta
    43861 gtaatttggg ataattctcc actagagatt caaagtaatg taatttacac tataataaat
    43921 ggcaaagtag tatatgagaa aaaataactt gacaattatt aaaaaattaa ctagtataag
    43981 agtgtaagga aaattaaaaa atcaaagaag atcttcgggg cagggcgaaa ttccctaccg
    44041 gcggtatagc ccgcgaacta cttaagtggt tgatttggtg agattccaaa gccgacagta
    44101 aagtctggat gagagaagat atttatggaa gataaataag tttattttag tttgtttaaa
    44161 ctatatccat aaaaactcga agatagggtc ttcgagtttt ttgtttttcc taaaaaatat
    44221 aaacatattg attaggggga aataattatg caaaacacag ccaaaagagg caatatgatt
    44281 tcaacaagaa ctttagtagt aatgtcaatt ttatctgcaa tatcttattt attaatgttt
    44341 attcaagcac caataccagg tatatttcca gatttcttaa aaattgactt atcagatatt
    44401 ccagcaatat ttggaggaat gtctctagga ccattagttg gatttggaat agttgtagta
    44461 aaaaatgtgt tacaagcaat aacagcaaca acaactggag gaataggaga atttgcaaat
    44521 atattaattg gtggttctta tgtaatgata ctttgttatt catataagag aagtggagat
    44581 ttaaaaggtg tattgattgg ttttgtatta ggaacaatat ctatgactat aattggttca
    44641 ttagtaaatt attttgtaat gttgccactt tatggtcaac ttatgggact agatgctata
    44701 ataggtttag gttcagctat aaatcctcaa gttcatgatt tattcacttt tgtaatttgg
    44761 atgatagctc catttaatgt gctgaaggct gttataatat ctttagtaac attgccactt
    44821 tataaaaaaa tgggaaatat aataaaaaaa taatttagac aagaaaatat aaaattaaat
    44881 gtattgaata gccatagttt atttataact tcagtcctat attgtgattg atatattaaa
    44941 tatattatgg ctttattaat ttatcaaaaa atgtatataa aataagaaat atttagacaa
    45001 atttaaattt taaaataaag gaagaattaa aacgattaac tattaaaata tgaaaaaggt
    45061 atctgcaata tacagagcga atttataata agcaaaaaat aaagttatta ttaaaaaaat
    45121 ataaagatta gtaatcaaat ttttaataat aactaattta ataatgatgg gagtgtatat
    45181 tcatgaactt atctaaaaag ataaaaaaat tatttttaga tgataaaggg tattatttta
    45241 atagtaattg gaaagatatt catattggta tttatgatac aaaactagga gattctttaa
    45301 agtatatcac taatgaatat attgaaaatc tggaaagggt aaaatatgat tatcgtgaat
    45361 taaaaaatat agaaaatcaa actgatggta tgtgtatttt agcaataagg cagaattaca
    45421 aggcattaga gtatgtgaaa agtcaaactg aagaattatg tttagaagca ataaagtata
    45481 attataaagc acttgaatat gtaaaagagc aaacagaata tatatgtctt gaagctataa
    45541 aaaaggattg taatgcactc aagtatgtac gaaataaaac agaagggctt attataaaag
    45601 taatacgtca ttctaccaat atagatgttg taagtatact aaaggcttta gagactcaaa
    45661 caagaagaat atgtttagaa gcaattaaaa aagatggcag gtgtcttgca tacgttagag
    45721 aacagagtga agaattatgt atagaggcca ttaaacaaaa ttataaagca ttaaaatatg
    45781 tagaaaatca aacggaaaaa atgtgtatag aagctgttaa acaaaatggt atggcattac
    45841 aatatgtaaa taaacaaacg gataaaatat gtatagaagc tgtcaaacaa gatggaagaa
    45901 gtctacaatt tgtcgataat aagacagaag aaatatgtat aaatgctata agatatttaa
    45961 ataaaaaata taatattaaa gatgtgctgt gttacgtaga taattataca gaagatatgt
    46021 gtatagagat tgtaagacaa aatggaaaaa tgcttatgta tataaaaaat caaacagaaa
    46081 aaatatgtat tgaagcagta aaagagaatt ataagtctct taagtatgta aaagagcaat
    46141 ctgagagaat atgtaaagaa gcattgaaac aaaattataa agcaaaagag tatgtaaaaa
    46201 ttgccattga tgattgtata taaggtgtat ctacaggcat caataatgaa agataaaaaa
    46261 agataaagac aagtacttta taataatctg ggcttatact ataattaatt aagcagtgat
    46321 ataaattcac atgacgatta tatcaaaatg gagatgaatt aatggcaaaa atatatttag
    46381 aaaatgaaaa taaaactaga gaaataggat ataaattagg taaactttta aaagaaggta
    46441 gtgttatctg tttagtagga gatttaggag ctggcaaaac tacaatgaca caaagtttgg
    46501 cagattcatt agggataaaa gattatataa caagtcctac ttttactata ataaatgaat
    46561 atgaaggaaa aatacctttg tatcattttg atgtttatag aataggcagt agtgatgaaa
    46621 tgtatgatat tggttatgat gaatatgtaa attcaaatgg aatctgtata atagaatggg
    46681 ctaatctaat agaagacata cttccaaaag aatatttgaa tatagaattg agatataaag
    46741 atgaaggaag agaaatgata ttaactccaa aaggagagtt ttataaagaa atagttgagg
    46801 agttgattaa ataatgaaaa tactaggtat ggatacatct tctatggcag caagcgtagc
    46861 agttgttgaa gatgataact taatatgtga atttacagcg aacaacaaaa aaactcattc
    46921 gcaaaagctt atgccaatga tagaaaatat gttgagcatg agtgatttaa gtataaaaga
    46981 tatggattta ttagctgtat gtataggtcc aggctcgttt actggactta gaataggtat
    47041 ggccacagta aaagctatgg ctcatgtaaa taatatacca ataatagcag taaattcttt
    47101 agaatctcta gcaaataaca tgaatttttg tgatagaaag atatgttgta tattagatgc
    47161 tcagaaaaat caagtttatt cagctaaata caagtttgaa aatggagaga tgatagaatt
    47221 agatggtgtt gatgttatag agtttgatgc tttagtagag gaaattgtat caactaatga
    47281 agaatttata atggttggag aagctgtata caaatataaa gataagctag agaatataga
    47341 aaatataaag ataccttcac cagctaataa tgtatctaaa gcaggaagtt tatgctcttt
    47401 agcattaaat aaatataata aaaatataga tgtacatact tgttatacaa taaatcctat
    47461 gtatatcaga aaatctcaag ctgaagtaca atatgatgaa aaaatgaaga ggctaaataa
    47521 tggaagataa tataaattta atagtaaatg atataaaaat agaagaaatg accattgaag
    47581 atatagatga agtttttgaa gtagaaaaaa attgttttga agattattgg tcaaaagagt
    47641 cttttagaaa agagttatct aatgaagtag caaggtatat agtagctaag ttagatggaa
    47701 aaattgttgg atatgtggga atttggctta tacttgatga aggacatata aataatgttg
    47761 cggttcatag tgactataga gggaaaaaaa taggggataa attgataaaa ggcatagttg
    47821 atttatgtaa agataataat attgcatcta tgacattaga agttagagca tcaaataaaa
    47881 tagcacaaaa tttatataga aaatatggat ttaaaatggg tggaataaga aaagaatact
    47941 acagtgataa taaagaagat gcaattataa tgtggaacca attaaaagag gtgtagagtt
    48001 tatgagtgat ataataacat tagctataga gagtagttgt gacgaaacag ccgcatcagt
    48061 tttaaaaaat ggtagagaag tactttcaaa cataatctct actcaaatcg agacacataa
    48121 aaaatttggt ggtgtagttc cagaagtggc ttcaagaaaa catgtggaaa atattgatat
    48181 agttgttcaa gaagctttag acaaagcaaa tatggatttt aatgatatag accatatagc
    48241 agttacatat ggacctggac tagtaggtgc tttactggtt ggtctatctt atgctaaagc
    48301 acttgcctat actttgaata ttccacttgt aggagtaaat catatagaag ggcatttaag
    48361 tgcaaactat atagagcata aggatttgaa acctcctttt ataacattaa tagtatctgg
    48421 aggtcatact cacttggttg aagtaaaaga ctatggaaag tatgagattt taggaaaaac
    48481 aagagatgat gcatctggag aagcctttga taagatttca agagcaatga atttaggcta
    48541 tccaggaggt cctattattg ataatctagc ccaaaaggga aataagcatg ctatagaatt
    48601 tccaagagcc tatttagaag aagatagtta tgattttagt tttagtggat taaaatcttc
    48661 tgtattaaat tatctaaatg gaaagagaat gaagaatgaa gaaatagtag ttgaagatgt
    48721 agcagcatct ttccaagagg ctgtagtaga ggttttatct actaaagcac taaaagcagt
    48781 aaaagacaaa ggatacaata tcataactct ttctggtggg gttgcttcta attcggggct
    48841 tagagctaaa ataacagagc ttgctaaaga cagtggtatt actgttaagt atcctcctct
    48901 aattttatgc acagataatg cagctatgat agggtgtgca ggatattata attttataaa
    48961 cggtaaaacc catgatatga gtttaaatgc agttccaaat ttaaagataa atcagtagag
    49021 taagataaat ttgtaaatat aaaagtattg tttcaaaaaa gtaagtagaa gtaaaattaa
    49081 atacaataaa aattaatata gtgtatatta aaacttgaag taaaactatt tataatcttt
    49141 atgtcccaat gaattgcaac acattttagg atataaaggt tgtctttggc ttagatttgc
    49201 ttcaagttta attttttctg tagaaagatt attttaaaaa gtgggtaata tacccttttt
    49261 tttcattctt aaaaagtttt atactaatta tagaaagatt aaggagggga agaaatgagt
    49321 caaagtaaag aagacaaaat aagaagtatt ttggaagcaa aaaatattaa atcaaatttt
    49381 caaaataaag aaaatttaaa tgaatttaat gaaaaaaaag caagtaaaag agcagaagac
    49441 ttattagatg tatattataa tacattatct actgctgata tggaatttcc atattggtat
    49501 aatagagaat atagaaaatc agatggtgat ataccagtag ttagaagagc taaagctctt
    49561 aaagctgctt tttcacatat gacaccaaat ataatccctg gtgaaaaaat agtaatgcaa
    49621 aagacacacc attatagagg ctcttttcca atgccatggg taagtgaaag cttctttgtt
    49681 gctcaaggag aacaaatgag agaagaggca aagaaattgg ccagtaacac agctgatgag
    49741 cttacaaaat ttgggtctgg cggaggaaat gttactgaaa gttttggaaa tgtcgtttca
    49801 atagctggta aattcggaat gagaaaagaa gaagtacctg tacttgtaaa aatggcaaaa
    49861 gaatgggttg gaaagtctgt tgaagattta ggttttcact atgaaaaaat gatgccagat
    49921 tatgatttaa aagaaaatct aatgagcacg cttatatgca tgtttgattc agggtataca
    49981 cttcctcaag gtagagaagt tataaattat ttttatcctt taaattatgg attagatgga
    50041 attatagaaa tggcaaaaga atgtaaaaag tcagttgcag gaaatgcatc tggagatggg
    50101 cttattggta tggaccgtct atatttctat gaggctgtta ttcaagttat tgaaggatta
    50161 caaacttgga ttttaaatta tgcaaaacat gcaaaatatt tagaaagtat agagacagat
    50221 ttagaagcta aaaaagaata ttcagattta gtggaaatat tagaacatat tgcacataaa
    50281 caacctcgta catttaggga agctcttcag ttgacttata caattcatat agcatcagta
    50341 aatgaggatg ctatatctgg tatgtctata ggtcgtttcg gtcaaatttt atatccttgg
    50401 tatgaacaag atatagaaaa aggattaatt acaaaagaag aggttatcga attattagaa
    50461 ctttatagaa taaaaattac ttgtattgac tgttttgctt cagcgggcgt aaatggagga
    50521 gtactatcag gaaatacgtt taacacttta tctataggtg gattaagaga agatggttct
    50581 acaggagcta atgagctaga agaattattg ttagaagcaa gtatgagatg tagaactcct
    50641 caacctagtt taactatgtt atatgatgaa aagcttccag aagatttctt aatgaaagct
    50701 gctgaatgta caaagcttgg ttctggttat ccagcatggg taaacaattc aaatggaaca
    50761 acatttatga tgaaacaatt tgctgatgaa ggtatgactg tagaggaagc aagagcattt
    50821 gcgttaggtg gatgtttgga aacgtctcca gggtgctgga aacagttaac tttaaatggg
    50881 aagacatact ctattgcagg tggagcaggt caatctgctg gttctggagt tcactttata
    50941 gctaatccta aaatcttaga acttgtactt atgaatggta aagactatcg tatgaatata
    51001 caagtgtttg agcctcataa taagccatta gatacttatg aagaagtcat agaagtattt
    51061 aaagattatt ataaacaagc aataaatgtt ttagaaagag caaataatat tgagttagat
    51121 atatggagaa aatttgatac ttctataata aattcactat taaagccaga ttgtctagat
    51181 aaaggtcaac atataggaaa tatgggatat cgttataatg caacattaaa cgtagaaact
    51241 tgtggtactg ttactatggt caattcattt gcagcactta agaaacttgt atatgatgac
    51301 aaagcattta cgatagagga aatgaaggat gccatattaa ataattttgg ctttaaagat
    51361 gcattagaag taggaaatta ttctatggca gaccaagtaa aagtagataa gactgggaag
    51421 tatgatgcta tttataaggc gtgtcttgat gcacctaaat atggaaataa tgatttgtat
    51481 gcagacaata ttcttaaaaa ctatgaggta tggttatcaa aagtatgtga agaagcacaa
    51541 tctttatatg ctaagaagat gtatccttgc caaatatctg tttctacaca tggaccacaa
    51601 ggagctgcta ctttggctac tccagatggt agattaagtg gaacaacata ttcagacggt
    51661 tcagtttctg catatgcagg aacagataaa aatggagtat atgcattatt tgaatctgca
    51721 actatttggg accaagcggt agttcaaaat tctcagatga atttaaaact tcatccaaca
    51781 actataaaag gacaacaagg aactaaaaaa ttattagatt taacaagaag ttatttaaga
    51841 aaaggcgggt tccatattca atataatgtt gtagattcag aaacattaaa agatgcacaa
    51901 aaaaatcctg acaattatcg tcaattaatg gtacgtgttg ctgggtttac tcaatattgg
    51961 tgtgaattag gtaaaccaat acaagatgaa gtaattgcta gaacagagta tgaaggggtg
    52021 taaaatatga gaaagcatag tgattgtatg aatttttgtg cagtagatgc aaccaaagga
    52081 atttgtagat tgtcaaaaca aatgattaat ttagatgatg aagcatgtcc agagataaaa
    52141 gtaatgccaa aatgtaaaaa ttgtaaaaat tttgttgaag ctaatgatga aggtataggc
    52201 aagtgtgttg gtctagaaaa ggaagactgg gtatattcaa cattgaatgc aattacttgt
    52261 gaaggacatg tgtttaatga gtagtcaaaa gaaattagaa ggtatgattt ttgatgtaca
    52321 aagcttttca gttcatgatg gtccaggttg tagaacaact gtatttttaa atggatgtcc
    52381 actgagttgt aaatggtgtg caaatccaga aagttggact gtaagaccac atatgatgtt
    52441 tagtgaatta tcttgtcaat atgaaaatgg atgtactgtg tgtcatggta agtgtaaaaa
    52501 tggtgcattg agctttaatc ttgataataa gccagttata gattggaaca tctgtaagga
    52561 ctgtgagagt tttgaatgcg tcaactcatg ttattataat gcgtttaaat tatgtgcaaa
    52621 agcttatact gtagatgaac ttgtgcaggt aattaaacgt gattctaaca attggagaag
    52681 taatggagga gtaacattta gtggaggtga gccactactt cagcatgaat ttttacatga
    52741 agtattatta aagtgtcatg aggtaaatat acacactgcc attgaaacga gtgcatgtgt
    52801 atcaagtgaa gtttttaata aaatatttga agatattgat tttgcattta ttgatataaa
    52861 acatatggat agagaaaagc acaaagaaca aacaggtgtg tataatgatt taatactaaa
    52921 aaatatatca aaccttgcaa attctgattg gaatgggaga ttagtactta gagtaccagt
    52981 tatctctggg tttaatgata gtgttaaaaa tataagtgat attatatctt ttatgcacaa
    53041 aaataattta gtagagatta atctattacc atttcacaga ctaggtgaat caaagtgggt
    53101 tcaattaggt aaagaatatg agtattctga taagggtgat gttgatgaag gtcatcttga
    53161 agaattacaa gatatatttt tggataatgg tatagcttgt tatgttggtc atgagacagc
    53221 tttctaaaat caaatacaag ttttaattaa aaaaggatgt tgtactaaaa gctagagtca
    53281 catccttttt tatgaggttt ttatgtataa agagttataa tatattttac taatttaaga
    53341 taaaaataat aattttttat aggtggaatc tttgcttgat atagaaatta ataataagat
    53401 tattccactt gttccctcta aaaaggatgt gttttccatt gtttctaaat tactaaatgt
    53461 tttaaagctg tatcttaatg ataaatcaaa caattgaaat atttcatcta aagttttttc
    53521 aaggttgtat agataaacat tatcatttgt ttttttgtac actgatgtca ttatagacaa
    53581 aatttaataa ctatccttta tattcttttg taaatagaaa tggaacataa aaaattaaag
    53641 aaaccataga tactattaat atgtcagaat aagtaataat tgactcttca acatttgtta
    53701 taagaaaagc gctattattt aaaatatgaa ttatagcaca aaaccagaca tttttagttt
    53761 tcatataaag atatcctaaa aatattgcaa ttccaactat atgtattgaa cgtaatatta
    53821 atcctaatat tggtgtttca ggtgtatata atgtaaatat tagtggtaag tgccacaaac
    53881 tccaacacat acctaatatt ataactccta gttttttacc aaacctatca aaaaaaatat
    53941 tctgtaaaaa acctctccaa gcatattctt cactaaaaaa aatataaata gtagttaatg
    54001 atgttattgg agtaaatata aattttatga tcccgctata actaggttga gttccttgta
    54061 gtattaagag aaatgatgtt ggaacaatta cagaaatcat aaagtaaata attaacaaag
    54121 aacctgcttt aatatttttt acgggatata aattttttgt atttgatgaa taaattataa
    54181 ttgataaact acttataaaa gttaatatta ggtttataat tgtaaattca ctattattta
    54241 ttagatttag caacctaaga ataattaata atgaataaat aagaaaaaat aatatgatta
    54301 gtgaataaaa tttatacata tcattattta atccttcagt ataaaatttt gcaatagcaa
    54361 ctgaagatag aggtaatatc atcatgaatg atgcaaaatt ttctggatta ataaatttat
    54421 tataatataa tagtaaacct aatgaataag ttaaaccaaa gtttataaag aaaaatatga
    54481 taatttgtaa tttttttctt tttatttcat ttttaataaa tatgggattt agaatattta
    54541 tccagattaa tt
//
LOCUS       DAMJVM010000015        54261 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.015, whole genome shotgun sequence.
ACCESSION   DAMJVM010000015 DAMJVM010000000
VERSION     DAMJVM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 54261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54261
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.015"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            11..87
                     /locus_tag="PC523_000975"
     tRNA            11..87
                     /locus_tag="PC523_000975"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Ile,seq:cat)
     gene            105..181
                     /locus_tag="PC523_000976"
     tRNA            105..181
                     /locus_tag="PC523_000976"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:139..141,aa:His,seq:gtg)
     gene            190..265
                     /locus_tag="PC523_000977"
     tRNA            190..265
                     /locus_tag="PC523_000977"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:223..225,aa:Lys,seq:ttt)
     gene            273..346
                     /locus_tag="PC523_000978"
     tRNA            273..346
                     /locus_tag="PC523_000978"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:305..307,aa:Cys,seq:gca)
     gene            354..430
                     /locus_tag="PC523_000979"
     tRNA            354..430
                     /locus_tag="PC523_000979"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:388..390,aa:Arg,seq:acg)
     gene            443..518
                     /locus_tag="PC523_000980"
     tRNA            443..518
                     /locus_tag="PC523_000980"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:476..478,aa:Val,seq:tac)
     gene            594..1007
                     /locus_tag="PC523_000981"
     CDS             594..1007
                     /locus_tag="PC523_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474526.1"
                     /translation="MYIVIGNEIVDSEELKVIIDKNSKFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLISEQCNLASLSDEEKFDEYMTLSDELALNLEELMPKYTIINARAYKL
                     DEVDGVVKIILAVAYADLGHLKLSDVLMRISRQVD"
     gene            1320..4751
                     /locus_tag="PC523_000982"
     CDS             1320..4751
                     /locus_tag="PC523_000982"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDF2474527.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHHEEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKAEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNIKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYSKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPEEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            4993..6921
                     /locus_tag="PC523_000983"
     CDS             4993..6921
                     /locus_tag="PC523_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDF2474528.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISNYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLKNSELVEVEKAKAGDIVVVTKINSLK
                     TGDTISIDKDAEALENIDFPKPQIYYAVTPKNKGDEEKVASVLNRLVEEDPTLHSYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGKSDVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFAEEIFGGSVPKQYIPAVEKGIKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASVAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
     gene            7378..7842
                     /locus_tag="PC523_000984"
     CDS             7378..7842
                     /locus_tag="PC523_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDF2474529.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGNQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            7849..8343
                     /locus_tag="PC523_000985"
     CDS             7849..8343
                     /locus_tag="PC523_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HDF2474530.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            8359..9384
                     /locus_tag="PC523_000986"
     CDS             8359..9384
                     /locus_tag="PC523_000986"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HDF2474531.1"
                     /translation="MKENIVMKSRVRLARNLNNYSFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAESDKSAVIVKKDKTVSIMINEE
                     DHIRIQTMCDDLNLEYAYSVANEIDDLLESSLEYAFSTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRIMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            9371..11818
                     /locus_tag="PC523_000987"
     CDS             9371..11818
                     /locus_tag="PC523_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDF2474532.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKGLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWSKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            11998..13371
                     /gene="radA"
                     /locus_tag="PC523_000988"
     CDS             11998..13371
                     /gene="radA"
                     /locus_tag="PC523_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDF2474533.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVTINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     EVVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVIKDTKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            13374..14444
                     /gene="disA"
                     /locus_tag="PC523_000989"
     CDS             13374..14444
                     /gene="disA"
                     /locus_tag="PC523_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HDF2474534.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAITNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            14533..15627
                     /locus_tag="PC523_000990"
     CDS             14533..15627
                     /locus_tag="PC523_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDF2474535.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYFIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKDIKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            15756..17933
                     /locus_tag="PC523_000991"
     CDS             15756..17933
                     /locus_tag="PC523_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474536.1"
                     /translation="MRKKIISFLATFIIILTSASQYIFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSVNENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVVFVELGDTYRLDLYKPNLNEKTYENMKNKIEKNIDVYLKNIFSMVGEND
                     TIYIASAFPSDLDYKNKRRLSPIVKLNGVGKGLLSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKKYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSVIVFSLLILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVLFVLGAFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILALMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYIIVPMITLSVNLTALKE"
     gene            complement(18112..18963)
                     /locus_tag="PC523_000992"
     CDS             complement(18112..18963)
                     /locus_tag="PC523_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2474537.1"
                     /translation="MNRNSFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCTYHNTSILLQVPMSFLNKYIPDFK
                     NCYFDFKVNANDNNYKRNSSKVKLILENMREIKLSSPPAANLQFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTNINYKSPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILETHGFTNTKLFYRIFKEKFKCTPKHI
                     RKNLPKI"
     gene            19180..20346
                     /locus_tag="PC523_000993"
     CDS             19180..20346
                     /locus_tag="PC523_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HDF2474538.1"
                     /translation="MYIKGVNLGGWLVLEKWMTPSLFEGTEAEDEYYLPRQLSRDVYE
                     SKIKTHRSEYITERDFAIIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFYWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGVCSWSQNPEYVEFTLNVLERLAKRYGMRRELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKDMARRSKPNSLEFLRDFYIKAYKVMRPYMS
                     EENVIVFHDAFELKSWKDFMKEEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIRDN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTAGGQSPLNGIESNIDKLSKDERRELYR
                     KIAKAQIDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            20368..22014
                     /locus_tag="PC523_000994"
     CDS             20368..22014
                     /locus_tag="PC523_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDF2474539.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVFGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDNGKKWISSLDWETREGYEKPGVICL
                     VEYCIEKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHGVTMGR
                     SKNIWGPYEKDLMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETPSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTEDNWLRMEDGSNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNKLGLQYYAPRISPLSFADVNSRTGYVRIRGQESRTSLNRVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIKGRKSYFEWSYDGEKYQRIGETFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIADESKDVD"
     gene            22112..23485
                     /locus_tag="PC523_000995"
     CDS             22112..23485
                     /locus_tag="PC523_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2474540.1"
                     /translation="MNKEVNVKATTIKYEKISFFERVSYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLISRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     ISAILLFSVPSSLGVTSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQDQYERGVL
                     SIFRMILATCGTLIINGLTLPLVEHFGNNLSAWTKTFFIFGIASIIVFFITFTGTKER
                     VKAVKQNKNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFAPINMVSNVSQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIAGQNYIG
                     VISASAIKGIGSAGIAATMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGGVLSIGGYVGTSATQSNSAIVSIKACFIYLPIFITILQIIIMFFYKLDKEYSTI
                     LNELNTK"
     gene            complement(23730..24908)
                     /locus_tag="PC523_000996"
     CDS             complement(23730..24908)
                     /locus_tag="PC523_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2474541.1"
                     /translation="MTINHTRLLQTFVELGINSYRQRGTDILKKLYFRFIAYFTTGLF
                     IFLVYFITLFILLNQLIYPEDSHKFLYVIDSSIIYFPSTLIFTFCFITGGILFCLFLV
                     RPLYLIISSISNLSRGNYTISEKAFTKNGKLKWYNFMYREVIINIKDLGNKLQETEIE
                     REKLEVAKTDWLASVSHDLKTPLSYITGYSSLMLNKNHSFSEEEMLTFLNNIYTKGIY
                     IEKLIDELSLTFFIDSHNKIHLKHTQVEIVSFLRNLIADITNNPKSEKHIFEFHTDLQ
                     HLNLVIDETLMYRAIYNLLINCVEHNPSGTEIDVVLTQKSDEIYININDNGIGINPET
                     LNDIFAKYYSSKNQNTQNKGLGLFIVKQIIDAHEGNISVSSIMGKGTSFQIMLKQHKP
                     "
     gene            complement(24832..25566)
                     /locus_tag="PC523_000997"
     CDS             complement(24832..25566)
                     /locus_tag="PC523_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2474542.1"
                     /translation="MNDKFKNSERILIVDDEEGLLTLLKITLNKEQYNNITCVSTGAK
                     ALECIENNQYDLIILDVMLPDYSGFDLCTNFRKKTIAPIIFLTARASDYDKLSGLAIG
                     GDDYITKPFNTMELVARIKAIFRRQQLERSVLCNHSENILVYNYGYIVLDMAHALLTV
                     SGKNVECTAKELELLEFFCQNPNRVFTTSHLYDKIWGLQGYGDEKTVTIHISKLRKKI
                     LDDNKPYKVITNLRGIGYKFIPPKGN"
     gene            25734..27185
                     /locus_tag="PC523_000998"
     CDS             25734..27185
                     /locus_tag="PC523_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HDF2474543.1"
                     /translation="MIKSLKSRGISILLCGALIVGVMTGCTSKATSPKSSTPKSTPKT
                     VEKVTRANCEKQLKEVKAKYKKNPNDEAIRLEYAQILFKLGNITEAQEILNPLLNSKK
                     PSPDAIFLSARIEYLNGNYTQAEKLYDNLIEQYPDKFKSKAEDELALTYYQTNQYQKA
                     DKLSSVKTNNALLEMMKAFKDKHPNKIDWNGKKETVIPFVTGDPLPIIPMEINGKRIN
                     TIIDNGAPMFVVDEKIAEEIGINLASKEENTKFAGGNSIGINFGQANSLTIGDVNMEN
                     IPVMLGPFDGFEKLFKDYGNVHGIMGTTVLKEFVPTMDYPSGQLILRPRNEVGRDNLN
                     KMLTNDKILEEVPFTLASTHFMFGKGSINQKVGLNFFVDSGLGDEKGAGIVLTEEIMN
                     SLKIPMPELKKAEEGKGGMAGSDYKEGYFNLSSYGLGDLQLKNVLGYYFTGDTLDYDE
                     GIGFVRDGLISHHYLKNYKWSIDFDSMKMTFSK"
     gene            27389..28342
                     /locus_tag="PC523_000999"
     CDS             27389..28342
                     /locus_tag="PC523_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDF2474544.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDSLTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            28380..29342
                     /locus_tag="PC523_001000"
     CDS             28380..29342
                     /locus_tag="PC523_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HDF2474545.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLRNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            29367..30353
                     /locus_tag="PC523_001001"
     CDS             29367..30353
                     /locus_tag="PC523_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDF2474546.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGVVPEDDYVIEIGKADIKKEGTDVTVITYGRMLQSV
                     EEAAENLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            30366..31412
                     /locus_tag="PC523_001002"
     CDS             30366..31412
                     /locus_tag="PC523_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDF2474547.1"
                     /translation="MVANKVKATPAARNQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTEKETIVSTNTSKFSE
                     KKETKIENENSSMFNTVEGVFEKPNPMRATVARRMSESYFSAPVFTFNIEVDATELKA
                     LRVKLIDTVKESTGVKLTMTDLIVMAVSRILPNHQALNSAWTDEGIFRYKDINIAIAV
                     GLDEGLYVPVVKSVNKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            31579..33309
                     /gene="lpdA"
                     /locus_tag="PC523_001003"
     CDS             31579..33309
                     /gene="lpdA"
                     /locus_tag="PC523_001003"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDF2474548.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAAIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEAKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVEVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPKMDKDLSE
                     SLKYSLGKKGINVLTKKKVSEFKEEGNNILVCIEDEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGMNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPADEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            33715..36663
                     /locus_tag="PC523_001004"
     CDS             33715..36663
                     /locus_tag="PC523_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2474549.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSMLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKELQDSDNL
                     KLYKESDDKKEEINNNRENTDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFALESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADESKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETKLTNKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKQKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIVGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LNSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRNISEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEAITIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGIDIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKYNNTKVNFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKVKLNQINNLIDTNQISETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKNPIVWSKNKVKIILLLAVDQKCFNPLSTLLSFIETDEFKQIKNIKDRDFIRE
                     MILDGVNENN"
     gene            36641..37105
                     /locus_tag="PC523_001005"
     CDS             36641..37105
                     /locus_tag="PC523_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2474550.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKASYIQ
                     AVIAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            37184..37486
                     /locus_tag="PC523_001006"
     CDS             37184..37486
                     /locus_tag="PC523_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2474551.1"
                     /translation="MGVTNVTKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLIKDVLEKLEL"
     gene            37548..38912
                     /locus_tag="PC523_001007"
     CDS             37548..38912
                     /locus_tag="PC523_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421211.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2474552.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIIMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMIIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGVAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSESAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     FIEE"
     gene            38947..39420
                     /locus_tag="PC523_001008"
     CDS             38947..39420
                     /locus_tag="PC523_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474553.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYINFNLNIYSF
                     LIIVCTYGIFNTFISSSNPKKIIIDNKCIHFSSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINEGNLFKGRYWIRTNMFNNSIELYSYLIELEECLYPDSLKFRNKRFENKNI"
     gene            39583..40233
                     /locus_tag="PC523_001009"
     CDS             39583..40233
                     /locus_tag="PC523_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDF2474554.1"
                     /translation="MLLDKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLIPSDIVIVDVDGNVIDGKWKASVDLPDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSNKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            40409..41341
                     /locus_tag="PC523_001010"
     CDS             40409..41341
                     /locus_tag="PC523_001010"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDF2474555.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVHHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLLTLGLGVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSDKSTESNATKSVSSKVTNIKFLLEKNISYFLDYCVEYLKSNIKNIKELELDEED
                     IKNVYNLVEDKYKKIEWTYGKTPKFQVVLPFNNKSKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            41616..42329
                     /gene="ispD"
                     /locus_tag="PC523_001011"
     CDS             41616..42329
                     /gene="ispD"
                     /locus_tag="PC523_001011"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDF2474556.1"
                     /translation="MYSVIIVAAGSGRRMHLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKELDKNCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERSLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            42326..42811
                     /locus_tag="PC523_001012"
     CDS             42326..42811
                     /locus_tag="PC523_001012"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDF2474557.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIENMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    42906..43113
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            43177..44892
                     /locus_tag="PC523_001013"
     CDS             43177..44892
                     /locus_tag="PC523_001013"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2474558.1"
                     /translation="MKMSKMFMPTLKEVPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSTEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNVASEKEEMKELEEVYTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGVVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDRDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAEKIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKFRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            45014..46459
                     /locus_tag="PC523_001014"
     CDS             45014..46459
                     /locus_tag="PC523_001014"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2474559.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRIEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAVCDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            46903..48384
                     /locus_tag="PC523_001015"
     CDS             46903..48384
                     /locus_tag="PC523_001015"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDF2474560.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHDEGVVLDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSIERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALKEKIEKAEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            48605..48760
                     /locus_tag="PC523_001016"
     CDS             48605..48760
                     /locus_tag="PC523_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474561.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            48905..50302
                     /locus_tag="PC523_001017"
     CDS             48905..50302
                     /locus_tag="PC523_001017"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDF2474562.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDGFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            50287..50700
                     /locus_tag="PC523_001018"
     CDS             50287..50700
                     /locus_tag="PC523_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDF2474563.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREHLIRQNTQKK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            50806..51564
                     /locus_tag="PC523_001019"
     CDS             50806..51564
                     /locus_tag="PC523_001019"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HDF2474564.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            51577..52314
                     /gene="rlmB"
                     /locus_tag="PC523_001020"
     CDS             51577..52314
                     /gene="rlmB"
                     /locus_tag="PC523_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDF2474565.1"
                     /translation="MANIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEHKYYELDELIDLVKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLGKSK"
     gene            52317..52844
                     /locus_tag="PC523_001021"
     CDS             52317..52844
                     /locus_tag="PC523_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDF2474566.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            52909..53556
                     /gene="sigH"
                     /locus_tag="PC523_001022"
     CDS             52909..53556
                     /gene="sigH"
                     /locus_tag="PC523_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HDF2474567.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            53630..>54261
                     /locus_tag="PC523_001023"
     CDS             53630..>54261
                     /locus_tag="PC523_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDF2474568.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSALMALEDPKSEWGDKIVELFEQIDEYIPAPERDTD"
BASE COUNT        22051 a         5820 c        10129 g        16261 t
ORIGIN      
        1 aatttaatgt gggccattag ctcagttggt tagagcgccc ggctcataac cggtaggtct
       61 ggggttcgag tccctgatgg cccaccacat taaattaaat tatggtgggt atagctcagt
      121 tggttagagc gccagattgt ggctctggag gtcgtgagtt cgactctcat tatccacccc
      181 aacttatatg gtctattagc tcagtcggta gagcacctga cttttaatca gggtgtcccg
      241 cgttcgagtc gcggatagat caccaaaaag gtggcgacat agccaagtgg taaggcagtg
      301 gactgcaact ccttgatccc cagttcgaat ctgggtgtcg cctccaataa aatgcgctca
      361 tagctcaact ggatagagtg tctgactacg aatcagaagg ttaggggttc gaatcccttt
      421 gggcgcacca tataaatagc gagggattat agctcagctg ggagagcacc tgccttacaa
      481 gcagggggtc acaggttcga gccctgttaa tcccaccagt gcaaacctct agtaatttta
      541 ctaggggttt tatttttgat tgaaaaagtt tttatattta ggaggtaaga aacttgtata
      601 tagtaatagg aaatgaaata gttgatagtg aagaacttaa agtaattata gataaaaata
      661 gtaaatttaa agtagaaaag gatttaagta aaagcacaaa aagagaagat gtaatagcat
      721 atcagttaag tatagattta aattatttag attctcttat aagtgagcaa tgtaatttag
      781 ctagtttatc tgatgaagag aaatttgatg aatatatgac tttgtctgat gaacttgcac
      841 tgaatttaga agaacttatg ccaaaatata caataataaa tgctagagca tataaattag
      901 atgaggtaga tggagttgta aaaataatct tggctgtagc atatgcagat ttaggacact
      961 taaaactttc tgatgtatta atgaggatat ctagacaagt agattaggat acgaatgaat
     1021 atgaactata ttaaaaaaat gtataaaatc ttacgtaaag atgaaaataa ttatgtaaac
     1081 acagaaaaaa acagtataaa atcaagtcaa ttgtagaaat tttcacataa attcatctga
     1141 aattctataa aaaatgcctt aattttaatg aggtaaatcg agtttacaca gtctaaaaat
     1201 tacttatatt tataaattta tcttattaat taaggaactt tcttaaacat cttcaaaaat
     1261 aaagtatttt agtctataat ctttaatatt acacaaacaa taaaggagaa gaatagagta
     1321 tgcttaaaaa gtttaataaa atactagtgg ctaatagagg cgaaatagct ataagaatat
     1381 ttagagcctg ttcagaattg gggataaaaa gtgttggtat atatagtaag gaagataaat
     1441 atggcttatt tagaacaaaa gctgatgaat catatttgat aggagaaggt aaagggccaa
     1501 tagatgcata tctagatatg gatggaataa tagacctagc aaagagaaaa aaagttgatg
     1561 ctatacatcc aggatatggt tttctagcgg agaatgcaga atttgctcgt aaatgtgaag
     1621 agaatggaat tacttttata ggaccatcat ctaaagtaat gaatatgatg ggggataaga
     1681 ttaactctaa aaaaatcgca aaagaagtta atgttcaaac tataccagga gtagaaaaag
     1741 caataagaag tactgaagaa gcaaaggaag ttgcaaacaa aataggctat cctgttatga
     1801 taaaagcatc taatggtggt ggcggtagag gtatgagaat tgtacatcac gaagaagact
     1861 tagagcttga atatgaaact gcttgcagtg aatcaagaaa agcttttgga gaagatatta
     1921 tatttataga aaaatatata gctgatccaa aacatataga agttcaaata ttaggagata
     1981 actatggaaa tatagttcac ttatatgaaa gagattgttc tgttcaaaga agacatcaaa
     2041 aaataataga atatgcacca gctttttcgc tagatgataa agtaagaaaa gagatatgtg
     2101 aagatgcagt taagctatca aaacatgtgg gttattctaa tgcaggaaca ttagaatttt
     2161 tagtagatgc aaatggcgga cattatttta tagagatgaa cacaagagta caagttgaac
     2221 atactgtaac agagatggtt acaggaatag atatagttca aagtcaaata ctaatagccc
     2281 aaggttacag tttagatagt gaagaaatca atataaaatc acaagatgat gtggaaataa
     2341 gaggttactc tatccaatgt agaataacta cagaagaccc taagaataaa ttcatgcctg
     2401 atacaggtaa aattcaagtt tatagaactg gttcagggtt tggtataaga ctagatggag
     2461 gaaatggatt tacaggggca aacataagtc ctcactatga tagtttattg gttaagacaa
     2521 tatcttggga tagaacattc caaggagcaa taaataaaac tataagatct ataaaagaac
     2581 ttagagttag aggtgttaaa actaatgtag gattcctagt taatgtttta aataatccta
     2641 tattctcaaa tggtaaatgt agtactaaat ttatagatga aaacccagat ttatttgaaa
     2701 taacagaaag taaagacaga ggaacaaaat tacttcagtt tataggagat gtaattgtaa
     2761 atgataatgc ttgcaaggaa aagccattat ttgatgcatt acatgaccca agaatggata
     2821 aggatggttc aaaagcagaa ggaagtaaaa tactttttga taagttaggt aaatctgcat
     2881 atatagaaaa gataaaaaat gataagaaat tattattaac agatacaaca atgagagacg
     2941 cacatcaatc attattagca acaagaataa gaacatatga cttattaaaa gctgctaagc
     3001 caacagaaaa atatcaaaaa gatttattct cacttgaaat gtggggagga gctacttatg
     3061 atgttgcata tagattttta aaagaatcac cttggcgtag attacaaaaa ctaagagaag
     3121 aaataccaag tataatgttc cagatgttgc ttagagcatc aaatggagtt ggatataaaa
     3181 actacccaga taatgtaata gaagaattta caaaagaatc tgcgagacaa ggtatagatg
     3241 tatttagaat atttgactca ttaaactggg ttgaaaatat gaaaccatct ataaatacag
     3301 ctttagaaac tggaaaaatt gttgaagcaa ctatgtgtta tacaggtgat atactagata
     3361 agactaaaac taagtataat ctagaatatt atattaaaat ggcacaagaa ttagaaagct
     3421 taggagcaga tataatagca ataaaagata tgtcaggact tcttaaacca tattcagcat
     3481 acactctagt taaagagtta aagaaaaata taaaagcacc aatacattta cacactcatg
     3541 atacaagtgg aaatggtgta gcaacttgtt taatggcatc agaagctggt gtagatataa
     3601 ttgacgctgc attagaatct atggcagggc ttacaagtca accatcactt aatgctatag
     3661 tagaggctct taaaaataca gaaagagata caggtataga tttatttgga tatgatgagt
     3721 taggtaaata ttataaggat ttaagaaaag tttatagcaa atttgaaagt gatttaacga
     3781 actcttgcgc agaaatatat aattttgaaa taccaggagg tcaatataca aacttaaaac
     3841 ctcaagctga tagtttagga ttagttaata gatttgatga agttaaagaa aaatataaag
     3901 aagctaatga agttgtagga gatataataa aagtaactcc atcttcaaag gttgtaggag
     3961 atttagctat ctttatgact aaaaataaat tagataaaga caatataata gaagaaggta
     4021 aaaatttatc attcccagat tctgttgtgg attattgtaa aggtatgatt ggtcaaccag
     4081 aaggtggaat accaaaagat ttacaagaag tagttctaaa aggtgaagaa gctatcacag
     4141 taagaccagg ttcattatta ccagaagaag attttgatga aatagctaaa tatctaaatg
     4201 aaaaatatga tataaatgct aacataagaa atgttataag ttacgcttta tatccaaaag
     4261 tatatgaaga ttatatcaaa catttacaac attataatga tatatctaaa cttgaaagtg
     4321 atgtattctt ctatggatta aataaaaatg aagaatgtga agtggaaata gaagaaggta
     4381 aagtattaac aataagatta gttgaaatag gtgaagttaa ggaaaatgga tttagaacta
     4441 taggatttga gctaaatgga atggttagag aagtagaaat aaaagataag aacttctctg
     4501 gaaaaattaa taatgttgaa aaagctgata tgaatgaccc tcttcaaatt ggagcaagca
     4561 taccaggaaa agttataaaa ataatggtta aagaagaaga tgaagtaaaa gcaaatcaac
     4621 cattaatcgt tatagaggct atgaaaatgg aaactataat agttgccaag actgatggag
     4681 tgattaagtc aatcaaagta aaagaagatg atatggttga agacaaacaa ttattaatga
     4741 taatgaagta attaaattaa aataaactaa aaagcactct gaaatgttta ataaaaaatg
     4801 ttttagagtg tttttattta ttttaattaa atatttgtta aaaaaaagag gacaatcatg
     4861 tccatatgta gaaataaagt tagtaaggaa aatgttatct aaaaattcta aatatagtaa
     4921 atattttccg taatatttct taggaaataa cagcaaagag aaaatacata aatttaaaag
     4981 gtgggtgctt atatgaaggt ttatgatagt aaaatgttga gaaatgtagc ggtattagga
     5041 catagcggat gtggaaaaac aaatctaatc gaaactatag cttacacagc aaacactaat
     5101 aagataccta aattaacaga caaggtaaat atgacttata gcatgggatt aatacctata
     5161 gagtataatg attataagtt taacttattg gataccccag gatattttga ctttagcgga
     5221 gacgttgtgt catctcttag agcaagtgat gcagcaataa tagtaataga tgcaacagcg
     5281 cctattcaag ttggtacaga gaaatcatta gaactaactg agagtattcc taagataatg
     5341 tttataaata aaatagataa tgaaaaagca agatataaag acgcaatagc aatgttaaga
     5401 gaaaagtaca acaataagat agttcctatg ataagtccta tatacaaaga taaaaacttt
     5461 gtaaagttac ataatgtttt tgaaaacata gatgatttag aaggtgaatt taaggaacaa
     5521 gctatgagtg ttaaagaagc attaatggaa cttatagcag agacagatga ccaaatatta
     5581 gataaatatt ttaatggaga agaattaact acagaagaaa tacaaaaagg tattattata
     5641 gggatacaaa gaggagatat tatacctgta atatgtggtt caactataaa taatatagga
     5701 acaaaagaaa tattagatac tatatcaaat tacctagaac ctatttttac ggaagaaagt
     5761 aaaccattta gaggattagt atttaagact atggttgacc catttgttgg taaaatgtca
     5821 tatataaaaa ttactgaagg tgtactttct aaagataaag atgtctttaa tataaataaa
     5881 aatgtaaagg aaaaaatagc taatatatat acattgaaaa atagtgaatt agtggaagta
     5941 gaaaaagcta aagctggaga tatagtagtc gtaactaaaa ttaactctct aaaaacagga
     6001 gataccatat ctatagataa agatgcagaa gctttggaaa atatagattt ccctaagcca
     6061 cagatatact atgcagtaac tcctaagaat aaaggtgatg aagaaaaagt agcatctgta
     6121 ttaaatagac tagtagaaga agaccctaca ttgcattctt atagaaacac agagacaaaa
     6181 caagcacttt taggagggca aggagaactt catataaaaa caattaaaaa taaaatgaaa
     6241 gataaatttg gtgttgatgt tgaattaaat gaccttaaag ttccttatag agaaactata
     6301 aaaggaaaat cagatgtaca aggaaaacat aagaaacaat caggtggtca tggtcaatat
     6361 ggagatgtaa aaataagatt tgaaagatgt gaatctgatt ttgaatttgc agaagagata
     6421 tttggaggtt ctgtaccaaa acaatatata cctgcagttg aaaaaggtat aaaagactct
     6481 atgcaaaaag ggatattagc aggttatcct gttacaaaca taaaagccac gctttatgat
     6541 ggttcatacc atgatgtaga ctcttctgaa atggccttta aaatggcagc aagtgtagct
     6601 tttaaaaaag gtatggaaga agcacatcca atactactag agcctataat gaaacttaag
     6661 ataactgttc cagaagaata tatgggagat gttatgggtg atataaataa gagaagagga
     6721 aaaatatttg gaatggaacc agatgacaaa ggtaaacaaa taatatttgc agaagctcca
     6781 caggcagaga catttaaata tgcaatagat ttaagagcaa tgacacaagg cagaggatac
     6841 tttgaaatgg agctagagag atatggagaa gttccatcac agtttgcgga aaaaataatt
     6901 gggttagcta ctgcaaagta atgaacaaaa ataaatagaa taataagtaa ttaatagata
     6961 ctattaaaaa tatgtttagg attttaacta ggtataagtt tatgttaagt aaacttatac
     7021 ctagttttta tatgttttta atagataaaa aaatataaaa tactttcttg aatattttaa
     7081 taaaaagaaa gtattttatt attattattt ttacaaatgt ttagcaataa agatgagaag
     7141 gtatatttaa aatatagagt atttaaaatg ttgaaatcta atcatgaagc aaaaaaatag
     7201 agtttggtta tcataaatat agtaaataaa aactcgtaat taatgaaaga ataaattcat
     7261 atctaaattt ctataaaaaa tttaaattta tacaatttag ggctttaaaa atttaaataa
     7321 taggtgtaaa ataatagtca aagaaagtca aaaataaaaa ggtgtgtggt gatattaatg
     7381 gcaactatga cagatataat tgaaaaattt attaaagatt tgatggaaga agataatagc
     7441 atacaaattc aaagaaacga actagcaaac ttgtttagtt gtgcaccatc acaaataaat
     7501 tatgtcttaa caacaagatt tacaatagat agaggatatt acattgaaag taaaaaaggt
     7561 ggcggaggat atgttcaaat agaaaaaata cgaaaaagta aagatggaca tattagagaa
     7621 ctgttaaatg aaaaaatagg aaatcaaata tcctataaaa aagcaaaaga attattggaa
     7681 ggtttaaaag agtcagactt gattaacgaa agagaattaa aattaatatt atatgcaata
     7741 gatgataaat cattatgtat gccaatatat gaattaaaag agaaggtaag gtctaatatt
     7801 ttaaagaata taataatagg attatttagt atagaggagt gataatttat gctatgtcaa
     7861 aaatgcaata agaataaagc gtctgtatat tataataaga tagtcaatgg agaaaaaaca
     7921 gagatgtatc tatgtagtga gtgtgcaaaa gagaatacag aaatgaattt taatttagat
     7981 atgccttttt caatgatgga tatattttct aatttaggtt ttcaacctaa aaaagaactt
     8041 gaggaaaagt tagtttgccc taaatgtaat acaacatata gtgaatttaa gaataatggt
     8101 agatttggat gtagtgaatg ttacaatgca tttagtagtc aagtaaatcc aatgcttcaa
     8161 aatatacatg ggcatataga acatacagga aaagcaccta aaaaatcttt ttataaaata
     8221 agtgtagaaa atgagattaa agaattaaaa gaagatttag atagagctat aaaaaatgaa
     8281 gaatatgaat tagcggctca atttagagat aaaattaaat acttaaaagg ttctattgac
     8341 taagattgga gtgatattat gaaagagaac atagtcatga aatcaagagt taggctagct
     8401 agaaatttaa ataactattc ctttccaaat aaattagata aagaatgtgc catggaaata
     8461 atagaaaaag taaaaaatgc atttataaat tctaacttag aacaaaaaga agaatttgat
     8521 ttctataaaa ttgaagattt agaccaatct aaaaaaatgc ttatggttga agaacatata
     8581 ataagcccgg atttagcaga aagtgataaa agtgcagtta tagtaaaaaa agataaaacg
     8641 gtaagcataa tgataaatga agaagaccat ataaggatac aaactatgtg tgatgattta
     8701 aacttagaat atgcatatag tgtagcaaat gaaatagatg atttgttaga atcaagttta
     8761 gaatatgcat ttagcacaaa gctaggttat ctaacatctt gtccaactaa tacaggaaca
     8821 ggaatgagag cctctgtaat gatgcattta ccagcactaa gtcagctagg ttatatggat
     8881 gaactttata aaatttcatc acaaataggt atagcaatta gaggtatata tggagaacga
     8941 acagaagcat taggaaatat atatcaaata tctaatcaac ttacacttgg aagaacagag
     9001 tctaatatta tagaaaatgt aagtggactt acaaaagatg caatttcaaa agaaataaaa
     9061 gcaagagaaa tattgcaaaa aaaattaggt ataaaattag aggataaaat atttagatcg
     9121 atagggacac ttgaaaattc aagaataatg agtagtgcag aggcaatgag ccacctatca
     9181 aatataaaaa tgggaataga aatgaactat atagataaac tagatttaag agctatagaa
     9241 caacttatga taggtataca accagcacat cagtcaataa tgtataagag tgatgatgtt
     9301 gagaatagag atataaatag agcaacatat attagagaaa ctttggaaaa attaagagga
     9361 gggatgaatt gtgaacttca atagatttac acaaagagct aaaaaagcta ttgatttagc
     9421 gtttgaatct gctaaaggtc tagggcataa tattgtcgga agtgaacata tacttttagg
     9481 tcttttaaga gaagaagaag gtatagctgc taaagttcta agtaaagttg gatttacaga
     9541 agcttattta gaaggcaaaa tagttgatat ggaaggtaag ggagaagaaa tctcagaaga
     9601 tatagtatta agtccaagaa gtaaacaaat acttgagtta tcaggcatgt ttgctaataa
     9661 attaaaaaca aattatattg ggactgagca tatattatta gcaattattc aagaaggtga
     9721 aggaatagct aataagattt taaattatgc aggagtaaat gataggactt tagcccaatt
     9781 aacaattgat atgatgggta ttagtgataa aaatcaatac aaagcagaaa atagttacac
     9841 gggcaaccag aatcaagcag aatctaaagt acttgataag tatggaagaa atcttacact
     9901 atatgcaaag cagaataaaa tagaccctgt aataggtaga gaaaaagaaa tccaaagagt
     9961 aatacaaata ttaagcagaa gaactaaaaa taatccagtg ttaataggag acccaggagt
    10021 aggtaaaaca gctatagctg aaggtcttgc tacaaatata gccttaggaa atgttccaga
    10081 aacactaaaa aacaaaactt tatactcttt agagatgggg tcattattgg ctggggctaa
    10141 gtatagagga gaatttgaag aaagaattaa agaagttgta gatgaagttg ttaaaaatgg
    10201 aaacataatt ttatttatag atgagatgca tacaataata ggagctgggt ctactggaga
    10261 aggctctata gatgcttcaa atatattaaa accagcttta gcaagaggag aaatacaagt
    10321 tataggtgca acaacaattg atgaatatag aaagcatgtt gaaaaagatt ctgctctgga
    10381 aagaagattc caaccagtta tggtagatga gccaactaaa gaggactcaa taaaaatatt
    10441 agaaggatta agagataaat atgaagctca tcataaagtt aaaataactg atgatgctat
    10501 aaaggcggct gtagaattat caactagata tatatcagac agatatctgc cagataaagc
    10561 tatagacttg attgatgaag ctgcatcaaa agtaagatta aaagaaaata cacctccatc
    10621 agagataaaa aaattagaac tagaaataga aaatatagat aaagaaaaag aagaagcagt
    10681 aagatgtcaa gactttgaga aagcagccaa aataagagat gaacaaggtc tacttaaaaa
    10741 acaattagaa gatgttagag agagatggag caagtcatct aagcattcag atttagttga
    10801 tggagaagtt attgctgaag tagtaggctt atggacagga atacctgtta ataaaattct
    10861 tgaagaagag gctgatagac ttttaagact cgaggaaata ctacacaata gagttatagg
    10921 ccaagaacaa gcagtaaaat ctatctcaaa ggcaatcaga aggtcaagag caggtcttaa
    10981 agaccctaat agaccaattg gttcattttt atttttaggt ccaacaggag taggtaaaac
    11041 agaattatct aaggcattgg cagaagtaca atttggagat gaaaatcaaa taattagaat
    11101 agatatgtct gaatatatgg aaaagcatgc tgtttcaaga atgataggtt cacctccagg
    11161 ctatgtaggt catgatgaag gtggtcaatt aactgaaaaa gtaagaagaa atccatattc
    11221 agttatctta tttgacgaga tagaaaaagc tcatccagat gtatttaata ttctattaca
    11281 aatcctagat gatggtagac taactgattc aaaaggaaga actgtagact ttaagaatac
    11341 cattgtaata atgacatcaa atgttggtgc atctacaatt ggtagacaaa aaactttagg
    11401 gtttagtata gctaaaggag acgaagaaga aaaatctcag tatgaaaaaa tgaaagaaaa
    11461 tataatgggt gaattaaaac aaagattcag accagagttt ttaaacagaa tagatgatat
    11521 aattgttttc cattcattaa atgaaaatca tatatcaaaa atagtacttt taatggcagc
    11581 aaaactacaa gagagattaa aagaaatgga tataaaatta gaaatgagtg atgaggctgt
    11641 taaattaatt tctaaatctg gatttgactt agaatatgga gcaagacctc ttaaaagagc
    11701 tttacaaaaa gagttagaag atgagttgtc tgaagcaatc ttaaaaggca atgtgaaaaa
    11761 gggaagtaat gtagtagcaa aagtcaaaga tgaaaaaata gtttttgaaa ctaaataatt
    11821 taatagagtt gcattttaaa atattgcaaa tataagcttt ataatctaaa caaaatttta
    11881 agggcaatga atctataata attcattgtc cttaattatt ttatttaatc tttacatatt
    11941 gtctttcaga tatgctaaaa ttatagtata gaataataga ttaaggatga taaaagtatg
    12001 gcaaaaataa aaacaaaata tgtatgtcaa tcttgtggat atgaaacagc taaatggctt
    12061 ggtaaatgtc cagaatgtac aaaatggaat acattcgtag aagaaataga ccaaaaaagt
    12121 accaaaaaag aggtctttat aatagataaa tcttcgtcta aaccagtaac tataaactca
    12181 atagaaagta aagaagaaga gaggtttaca acagatataa atgaattaga tagagtgctt
    12241 gggggaggta tagttaaagg ttctttagta cttgttggtg gagaccctgg tataggtaaa
    12301 tcaacacttt taatccaagt atctagcaat gttgctaatt taggaaaaac tgttttatat
    12361 ataactggag aagaatcaga atctcaaata aaaatgagag ctaaaagatt aggtattaac
    12421 tcagaaaatt tatatatatt tgctgaaaat aatttaagta taattgaatc ttatttagaa
    12481 gttgtaaatc cagaattaat aattctagac tcaattcaaa ctgtcttcag tccagaaata
    12541 tcctcggcac caggaactgt cagccaaatt aaagaaggta cttcaaaatt catgaaaatt
    12601 tctaaaaaaa tgggaatttc aacattcata gttgggcatg ttacaaaaga aggttcacta
    12661 gcaggaccaa aattattaga gcatatggtt gatactgttc tatattttga aggagaaaga
    12721 tataatactt atagattagt aagagctgtt aaaaatcgat ttggctcaac taatgaactt
    12781 ggagtatttg aaatgaggga tttaggatta gtggaacttg ataatccgtc taaaatttta
    12841 atctctgaaa aaccaaaaga tgtagcaggt tcagtaataa tatctactgt agaaggtacg
    12901 agaccaatgt tacttgaatt acaagcatta gtttctccta caagttttgg tataccaaaa
    12961 agaacatcta caggagtcga ttacaataga gtaggtatgt tattagcagt attagaaaaa
    13021 cgtgttggtt tgcaaataca aaatcaagat gtctatataa acatagttgg aggaataaag
    13081 ataaatgaac cttcaattga cttagggata gctatttctg tagcatctag ttttaggaat
    13141 ataccaatag atgaagatat agctgtcaca ggagaagtgg gtcttacagg agaagttaga
    13201 gcagtaagct ttatagaaaa gagaatagct gagtgtaaaa aacttggatt taaaaagata
    13261 gtagttccta gaagtaatta tgatgttata aaagatacca aaggcataga aatatggcca
    13321 gtagataact taagacaagc tataaatata gtgcttggga ggaatcaata aatatggaga
    13381 attttctaga taataaaaat atgctatatg cattaaaaat gatatctcct ggaactccac
    13441 ttagattagg tctaaacaat gtactaagag ctaagactgg tggattgatt gtaattgcaa
    13501 cgaacgaaga tgtaatgaaa atagtagatg gaggatttgc tataaatgca gaatattcac
    13561 catcatatct atatgaatta gctaaaatgg atggagctat agttttaagt ggtgatgtaa
    13621 agaaaatatt atttgctaat gcacaactta tacctgatta ttttatagaa acatcagaga
    13681 caggaacaag gcatagaaca gcagaaagag tagcaaagca aactggtgct atagtcatag
    13741 ggatttcaca aagaagaaat gttataacag tttatagagg aaatgagaag tatgtggtcg
    13801 aagatatatc taagatattt actaaggcaa atcaggctat acaaacttta gaaaaatata
    13861 agacagtatt agaccaagct ataacaaatt taaatgcctt agagtttaat gatttggtaa
    13921 ctatttatga tgtcgcatta gtcatgcaaa agatggaaat ggtaatgaga gttacaagta
    13981 taattgaaaa atatgtgata gaattgggtg atgaaggaac tttagtaagt atgcaattag
    14041 aagaattaat gggtacgacc agaatagacc agaaactaat attcaaagat tataataaag
    14101 aaaatacaga aataaaagaa cttatgaaaa aggtcaaaaa tttaaattca gaagaactaa
    14161 tagaattggt caatatggca aaactattag ggtatagtgg tttttcagaa agtatggata
    14221 tgcctataaa aacaagaggc tataggattc ttagtaaaat acatagacta ccaacagcaa
    14281 taatagaaaa cttagtaaat tattttgaaa actttcaaca aattttagat gcatctattg
    14341 aagaattaga tgaagttgaa ggaataggtg aaataagagc aacatatata aaaaatggac
    14401 ttataaaaat gaaacaatta gtattattag atagacacat atgatgaatt ttaaatgaat
    14461 tttactactt ttttcttgaa tataatatac actttggtct ataataaata agtataacta
    14521 gggggtgaag cattgataag gaaagtaaca agaatattgt ttattttact tggatttaca
    14581 ataggtataa caacctattt aacattaatg aaagattttg aaatattaac atttggaaaa
    14641 gaaacatatg gttatatcgc agctgtagta gcaggtataa taatagccat tttaggatat
    14701 tttatagaac catgggttgt aaataaagta aaagaaattg ctaaaatagt agataaagaa
    14761 ttgtcaaaat atcctcagac tgatatattg ttagggtcta tgggtcttat agtaggattt
    14821 gttattgcat atttattaag tggattagta aatagaatac caatagtagg tggaatacta
    14881 tccttactat tatatttatt cctaggatat cttggaatga aagttgcgct taaaagtaaa
    14941 aatgatttat ttaatattgg aaaactagga agattagcaa atcctattaa ggataaagat
    15001 aaggataaag aaaataaaaa agatataaaa gccattcctc caaaagtatt agatacaagt
    15061 gtaataattg atggaagaat agctgatatt tgtaagactg ggtttataga aggaaaactt
    15121 attattcctg cttttgtttt ggaggaacta agacacattg cagattcttc agatgattta
    15181 aaaagagtaa gaggtagaag aggtctggat atattaaata taattcagaa agaattaaat
    15241 atagaagttg aaataagtga gagagacttt gatgatatag cggaagttga tagtaaatta
    15301 ttaaagcttg ctcaggtttt aaatggaaaa gttgttacaa atgattataa cttaaataaa
    15361 gttgctcaat tccaaggtgt tgaagtatta aatataaatg agttagctaa tgctataaaa
    15421 ccagttgcta taccaggtga ggatatggtt gttcaagtgg ttaaagaagg aaaagaggct
    15481 caacaaggtg ttgcatatct agatgatgga actatgatag ttgttgatgg aggaagaaaa
    15541 tatatgaatg agactataaa agtacttgtt acttctgttc ttcaaactcc tgcaggtaga
    15601 atgatatttg caaaacctaa aaattaatat gttcaaagaa acctataatt tgggctgttt
    15661 attttaaata gatggtccaa atttatttat ttataacgat aatgtttaat gtattcttaa
    15721 agatggggat ttaaatcaga aagcagggag aatttatgag aaaaaagatt atatctttct
    15781 tagcaacatt tatcataata ttaacatcag caagtcagta tatttttgca gatgatatat
    15841 caacacgtgg aaaagtaata tttatagata tgaatagaac tagtatgagt aatatgttaa
    15901 gaataaaatc attaagagaa gaactagaca atagagggta tatcggtttg atgaatataa
    15961 gaggcgataa aggaagtgat gatagacggt catatgctag tatgggtgct ggtggtagag
    16021 ctaatgtggc taatgaagaa gatattaatt ttgaaagctc gagtaaggat agaaatatag
    16081 tttttgaatc agctacagga aagagcgcaa aaggtataaa taatttaact ataaataaat
    16141 ctgtaaatga aaatttaaac tttggagagt atggttctgt attagggtca ttaggacaaa
    16201 gtttatctga taatggacta aaagcatctg tattgggaaa ttcagatata attgaaaatg
    16261 gccaacttat aaaaaataga aacttatgtc taactgcaat ggatgaatat ggaagaatac
    16321 ctaatggaaa tgtagatact ataaataaaa aagacttatc tatgccttat ggaatatcaa
    16381 ctgattatga taaattgatt gtagagacca aagaagctta caaaaataat gatgtggttt
    16441 ttgtggagct tggagataca tatagattag atttatataa accaaattta aatgaaaaaa
    16501 catatgaaaa tatgaaaaat aaaatagaaa aaaatataga tgtatattta aaaaatatat
    16561 tttctatggt tggagaaaat gacactatct acatagcaag tgcatttcca agtgatttag
    16621 attataaaaa taaaagaaga ctttctccaa tagtaaaatt aaatggagta gggaaaggtc
    16681 tattatcatc ttcaactact agaagagaag gtattgtaac aaatttagat gttggggttg
    16741 aaatattaga caactttaat ataaaaaatc aaaatatggt tggaagaaaa tatgaactta
    16801 ttaacagaga tgataataaa gaatttttga tggatgaata tcaaaagata gtatctatct
    16861 caagcataag gtctacaatt ctgaatggtt ttgtaagcat agttttcttg tcatggatag
    16921 ttgctatgat tgcaatttta tttagaaagc atatatcaaa aaaatataaa gaaacaacat
    16981 tttttatatt aaaggaactt ttaaaaatag gaatagtaat gccactgtca tttatgataa
    17041 caccaataat gaatttcaaa acaccattag caatatcttt aggaataatt ataataacac
    17101 ttactatata tcttataagt aaagttttaa taaagaatga tttaaaaaac atgctgtttt
    17161 ttactggatt aactatagta attatggtta ttgatgctgg atttggctca tatttaatga
    17221 aaagcaatgt tatgagttat gactgtataa ttggagctag atattatgga gtaggaaatg
    17281 agtatcaagg tgtggcaatt ggtagtgcaa tatttacatt tgcaatttta cttacatata
    17341 aaaacatacc aaagtggtct gtaatagttt tttctttgct tatcttaatt acaagtgcat
    17401 ctccaattat gggagcaaat gttggaagtg ctatttctga atgtgttgca tttttactat
    17461 ttatactatt aatatataat gtgaagatag acttcaaaaa aattgtatta ttaggtatag
    17521 cagtattatt tgtattagga gcttttgtag caatagatat gattttggga agtaattcac
    17581 atttaggtat gtttgtaaaa gaaatttact ttaatggacc aggagagata atacaaacat
    17641 ttagtagaaa gatagagatg aatttaaagc tagctcaaac cagtgcatgg gtaaatatac
    17701 tgttaactgg aataggggta attttagctc ttatgattaa tcagattaga tactttaagc
    17761 aacttatgga tgagtatcca attgtattta aagggtttat agcatcaata gcgggatgtt
    17821 tagtaactct tttagtaaat gattctggga ttgtgtcatc agctacagcc tttatttata
    17881 taattgtacc tatgataaca ttaagtgtaa atctcacagc attaaaagaa taagcactat
    17941 atgagcacta tgtaaatatg aacacattaa tttaacttcc ttctttattt tataaagaag
    18001 gaagttaata aaaaataaat tataataaaa aagagtttat ctcaaaattg atttttctaa
    18061 tcaaattttg gggtaaactt tttttattat aaaggcaaat aacatataat cttatatttt
    18121 aggtagattt tttcttatat gtttaggggt acatttaaat ttttctttaa aaatcctata
    18181 aaataatttt gtattagtaa atccatgagt ttctaatata ctgcataagg tatcatttgt
    18241 attaattaag tctttataaa tatgagaaat acgtacatca tttaaatact ctaagtaagt
    18301 ttgccccata tattgtttaa attttctaca aaaatattct ggttgtaagt gtgccacctt
    18361 agcaattttg ttaatagaaa taggagattt gtaattaata tttgtatatt ctaaaactgg
    18421 ttcaaatttt gacaaatcaa gtactgtttt tttcatattt ttattaccta cagatatttt
    18481 aaagtttgta tataattcaa ataaaagttc aaataataag cttgtaaatt gcagattagc
    18541 tgctggagga gaactaagtt taatttctct catgttttct aatataagtt taactttact
    18601 tgaatttctt ttatagttat tgtcattagc attaacctta aaatcaaagt agcaattttt
    18661 aaaatcagga atgtatttgt ttagaaaaga cattggtact tgcaataata tagaggtatt
    18721 atgataagta catctagttg agtgaataat tccagaatta atgaacatac actcgttagc
    18781 ttttaagtta taagtgtaac cttccatgtt aatttgtaaa tcaccagagg ttatataaat
    18841 tatttctaat gagttatgcc aatgatttgc tacataacta ccactatcat caaatagttt
    18901 aaaattaaca tctaaattat caataggttc aattatttca tgtgaaaaac tatttctgtt
    18961 cataataatc tcctccttat aactttaaat tataactagt aaatttataa tattcaatat
    19021 taaaaagata atatagacta ataaattaga caataaacat acttaagaaa acagataaaa
    19081 tcaaatataa taactgtata tttaaataat ttaaaaaaat tattttagta taagtgtaaa
    19141 atatacctat ttaaataaga taagagggga ggactttatg tgtatataaa aggtgtaaac
    19201 ttaggaggtt ggttagtttt agaaaaatgg atgaccccaa gtttatttga aggtacagaa
    19261 gccgaagatg aatactactt accaagacaa ctttcaagag atgtatatga atctaaaatt
    19321 aagactcatc gtagtgaata catcacagag agagattttg caataattaa atctatggga
    19381 tttaattcag ttcgtatacc agtaccatat tttatttttg gagattgtga accctttata
    19441 ggttgtgtta aagaacttga taaagcattt tattgggcag ataagtatgg acttagtata
    19501 ttgatagatt tacatacagt tccaggaagc caaaatggat ttgataatgg aggaatatca
    19561 ggggtatgta gctggtcaca aaacccagaa tatgtagagt ttacattaaa tgtactagaa
    19621 agacttgcaa aacgttatgg gatgagacgt gaattgtatg gaatccaaat tttaaatgaa
    19681 cccataacag aaagaatgtg gaatataatg aatgttccaa atcgatttaa ggctgtagat
    19741 aaggatatgg caagaagaag taaacctaat tcattagaat ttttacgtga tttttatata
    19801 aaagcatata aagtaatgcg tccttatatg agtgaggaaa acgttatagt atttcatgat
    19861 gcatttgaat taaaatcatg gaaagacttt atgaaagagg aagaatttaa aaatgttgtt
    19921 ctagatacac accaatattt gatgttagct gaagcagatg gatgtgaaca aagtattgat
    19981 agttatctca aatatattag agataattat gctaaagata ttttacaaat gcaaaagtat
    20041 tttcctgtta tatgtggaga atggagctta tttaattcat atgcttgtgg tatagataca
    20101 gctggaggtc aatcgcctct aaatgggatt gaatcaaata tagataaatt atcaaaagat
    20161 gaaagaagag aattatatag gaagatagca aaagcacaga ttgatgcatg gagaaatgga
    20221 agtggtcact attattggaa ctataagtta ttactagata cagttaatga agaaggctgg
    20281 ataggttggg atagttggga cctaggcaaa tgtgtagctc aagaatggta tcctatagaa
    20341 tattaaacta aaattggagg aaaagatatg attaaaaacc ctatacttcc aggctttaat
    20401 ccagatcctt gtatttgtag aaagggagat gattattatc ttgtagtttc atcttttgaa
    20461 tggtttccag gtatacctgt ttatcactct aaagatttaa aaaattggga gctatacact
    20521 catattttaa cagatgaaac aaaaatagat ttaaagaagc ttccttcatc taaaggaatt
    20581 tgggcacctt gtttgactta ttgtgaagaa gaagatttat tttacatagt ttttggaatt
    20641 atgaactcta tgaatgcaag atattttgat gttgataact atttaattac atcaaaagat
    20701 ataaagggag agtggagtga accagtatat ctacattcat caggttttga tgcgtctatt
    20761 tttcatgatg ataatggtaa aaagtggatt tcgtcattag actgggagac tagagaagga
    20821 tatgaaaaac ctggtgttat atgtttagta gaatactgta tagaaaaaaa agaaatagta
    20881 ggatatccaa agagaatatg gtcaggagga actgatagag gttgtattga ggcaccacat
    20941 ataaccaagc gtggagatta ttattatata atgtgtgcag aaggtggaac tggatatggt
    21001 catggtgtaa ctatgggaag atctaaaaat atatggggac cttatgaaaa agatttaatg
    21061 aatccaattg taacttcaat tccaggagat ttttatgaaa ggcatgaccc tgaccatcta
    21121 aagcctaaat actataatcc agaatcaaag cttcagaaat ctggtcatgg aagctatata
    21181 gaaacaccat caggtgaagt gtatttagtt catctcacat ctagaccatt tgtaccagag
    21241 ttaaggtgta ctttaggtag agaaacagct attcaaaaaa tgaaatggac agaggataat
    21301 tggcttcgaa tggaagatgg aagtaatttg gcaaaagaat atgtatcaga aagtaagcta
    21361 gaagagcatt tagtttcatc tataccaagt tttgatgact ttgattcaaa taaattaggt
    21421 ttacaatact atgcccctag gatttcgcca ttatcatttg cagatgttaa ttctagaaca
    21481 ggatatgtaa ggattagagg gcaagaatca agaacatcat taaatagagt gagcatatta
    21541 gcaagaaagt tgacttcagt atatgcaaga ataactacta aaatggagtt ttatccagag
    21601 gtacatcagc atagcgcagg tttgataatg tattatgata atatgaatta tataaattta
    21661 agaaagtact atagtgaaac attgggtcaa agtgcactat caataataca tcttgaaaat
    21721 ggagagaaaa cagagttttt aaatactagg ataccaataa aagatatacc aatatatctt
    21781 cgtctgtata taaaaggtcg taagtcgtat ttcgaatgga gttatgatgg agaaaagtac
    21841 caaagaatag gagagacatt tgatacaact aagttttcag atgagtactg taaatatgga
    21901 gaatttacag gtacatttat aggattaact tgtgcagatc gtgtaaagca taaacactat
    21961 gctgattttg atttctttga atatatagca gacgaatcaa aagatgtaga ttaataaaat
    22021 tattactaaa atttttagag cataaggtaa aacttatgct ctgaaataag aaacattaga
    22081 aggtaaatct tacaagaagg agtatggtat tatgaataaa gaggttaatg ttaaagcaac
    22141 tactattaaa tatgaaaaga tttcattttt tgaaagagtt tcttatgggt gtggggattt
    22201 aggatgcaat atcatatatt cagcaatgtc agcattttta ttgttttatt acactaatta
    22261 tgcagatgtt agtgcagctg ctgtaggtag tattatgctt atatctagga tattggatgg
    22321 gtttagtgac ttgacaatgg gaattatagt ggatagaact aaatctaaat atggtaaggc
    22381 taggccttgg attttaagaa tggcgattcc ttttgccata tcagcaatat tattattttc
    22441 tgttccatca agtcttggtg tcacttctaa attaatttat atttttatta catataactt
    22501 agtttctact gttatttata cagcaatcaa tgtgccctat gcaactctaa attcacttat
    22561 aactcaagac cagtatgaaa gaggagtatt aagtatattt agaatgatat tagccacttg
    22621 tggtacttta ataataaatg gacttacatt acctctagtt gagcattttg gaaataattt
    22681 atcagcatgg actaaaacgt tttttatatt tggaatagca tctataatag tattttttat
    22741 aacatttact ggaactaagg aaagagtaaa agcagttaag caaaataaga atgaagtaat
    22801 accatttaaa atcggtatta aatctttatt tagaaataag tactggatac aaataacatt
    22861 atgtttagtt tgtatattta ttgtatttgc aataaatggt ggttcatctg tatattatgc
    22921 aaagtttatt ttaggagatg aaaaattatt tgctcctata aacatggttt caaacgtatc
    22981 tcaaattata gctatgttta tggttgctcc ttttataaaa aaatttggga aaagaaatgt
    23041 attaatagta ggttcaataa tattaatatc atcaaatgtt atgtttataa tagctggaca
    23101 aaactatatt ggagtaatat ctgcaagtgc tataaaggga atagggagtg ctggaatagc
    23161 agcaacgatg tttgctattg tttcagatac tattgaatat ggagaatgga agactggata
    23221 tagaactgaa ggtcttataa atagtgcatc tagttttggg tttaaagtcg gaaatggact
    23281 aggttcagca atattaggtg gagtattatc aattggtggt tatgtaggga catcagctac
    23341 tcaaagtaat tcagctatag tatcaataaa agcatgtttt atatacctac ctatatttat
    23401 aaccatatta caaattataa taatgttttt ttataagctt gacaaagaat atagtactat
    23461 acttaatgaa ttaaatacta aataataaaa tattttctat tcagtcttag atttaattaa
    23521 ggattattat agtttattat ttatgaaaaa ctattatata ttcatgatat attaaagcgt
    23581 ttttagaaaa ataaaaaaga aaaactaata ctaaaaatat aaatatatcc agtaaaaaac
    23641 atcttaaaat ctatttattt ggattctaaa agaataaagg agatacctta aaataaaaat
    23701 ttgagttacc tcctttttta ggcaataatt taaggtttat gttgtttcaa catgatttga
    23761 aatgatgtac cctttcccat aatgcttgaa actgaaatat ttccttcatg tgcatcaata
    23821 atttgcttaa caataaataa tcctaacccc ttattttgag tattttggtt tttgctacta
    23881 taatacttag caaaaatatc atttaatgtt tctggattta tcccaatacc attatcatta
    23941 atattaatgt aaatttcatc agatttttgt gttaagacaa catctatttc tgttccagaa
    24001 ggattgtgtt caacacaatt aattaaaagg ttataaatag ctctatacat tagtgtctca
    24061 tcaataacca aatttaaatg ttgtaaatcc gtatgaaatt caaatatatg tttttctgat
    24121 ttcgggttat tggttatatc agcaataagg tttcgaagaa aagaaactat ttcaacttgt
    24181 gtgtgtttaa gatgtatttt gttatgtgaa tctataaaaa acgtcagact taactcatct
    24241 attaattttt caatatatat tccctttgta tatatattat ttaaaaaggt taacatctct
    24301 tcttcactaa aactgtggtt tttgttaagc attagagatg aatatccagt gatatatgac
    24361 aagggggttt ttaaatcatg cgacacacta gcaagccaat cagtttttgc aacttcaagt
    24421 ttttctcgtt caatctcagt ttcttgaagc ttgttgccta agtcttttat atttataata
    24481 acttctctgt acataaagtt ataccatttt agctttccat ttttagtaaa agctttttca
    24541 gatattgtat agtttcctct agataagttg cttattgaac taattatcag gtagagtggt
    24601 cgaacaagaa ataaacaaaa tagtattcct cctgtaataa agcaaaaagt aaatataagt
    24661 gtgcttggga aatagataat agacgaatca attacatata aaaatttatg tgaatcttca
    24721 gggtatatta attgattaag caatatgaat aatgttatga agtatactag gaatataaag
    24781 agacctgttg taaagtaagc aatgaaacga aaatataatt ttttcaatat atcagttccc
    24841 ctttggcggt atgaatttat acccaattcc acgaaggttt gtaataacct tgtatggttt
    24901 attgtcatcc agtatctttt ttctaagttt ggatatatgt attgtaactg tcttttcatc
    24961 gccatatcct tgtaatcccc aaattttatc atacaggtgt gatgtagtaa agaccctatt
    25021 tggattttga caaaaaaatt ctagtagttc taattctttg gctgtacatt ctacattttt
    25081 accacttaca gtaagtagtg catgagccat atcaagaaca atatatccat aattataaac
    25141 taaaatattt tctgagtgat tacataatac agaacgttcg agttgttgac gtctaaaaat
    25201 agctttgatt ctagcaacaa gttccattgt attaaatggt tttgtaatat aatcgtcacc
    25261 accaatagca agtcctgata atttatcata atcactagct cttgcagtta gaaaaatgat
    25321 aggtgctata gtttttttgc gaaaatttgt acataagtca aatccactat aatctggtag
    25381 cattacatca agaatgataa ggtcatattg attattttct atacattcaa gggctttggc
    25441 ccctgttgat acacaggtaa tattattgta ttgctcttta tttaaggtta ttttcaatag
    25501 tgttaaaagt ccttcttcat cgtcaacaat aagtattctt tcagagtttt taaatttgtc
    25561 attcataata attcatctcc cagtatacaa aaatagtatc attataatac catacatgca
    25621 aagtgtaagc aataaattat tcttttgatt ttactttaac tttactttta ttttactttt
    25681 gtactgtatc ttatagataa caagaaagaa aaaaataaaa ataggaggta aaaatgataa
    25741 aatcactgaa atcaagagga ataagtattc tattatgtgg agctttgatt gttggtgtaa
    25801 tgacaggatg tacatctaaa gctacatccc caaaaagctc aacacctaaa tcaactccta
    25861 aaacagtaga aaaagttaca cgtgcaaatt gtgaaaaaca actaaaggaa gtaaaagcaa
    25921 agtataagaa aaatccaaat gatgaagcta tcagattaga atatgctcaa atactcttta
    25981 agttgggaaa tattacagaa gcacaagaaa ttctgaatcc gttactaaat tcaaagaagc
    26041 cttcaccaga tgctattttt ctttctgcac gaatagaata tctgaatggt aattatactc
    26101 aggctgaaaa actatatgat aatcttatag agcaatatcc tgataaattc aaatcgaagg
    26161 ctgaagacga gttggcactt acgtattatc aaacaaatca atatcaaaaa gctgacaagt
    26221 tatcctccgt gaaaacaaat aatgctctat tggaaatgat gaaagctttt aaggataaac
    26281 atccaaataa gattgactgg aatggaaaaa aagaaactgt aattccattt gtaacaggtg
    26341 acccactacc tattatacct atggaaataa atggtaaaag aataaataca attattgata
    26401 atggtgctcc aatgtttgtt gttgatgaga aaattgcaga agaaattggt attaatctag
    26461 cttcaaaaga ggaaaatact aaatttgctg gtggtaatag tattggaatt aattttggtc
    26521 aagccaattc acttaccata ggagatgtaa acatggagaa tattcctgta atgttaggac
    26581 catttgatgg atttgaaaag ctgtttaaag actatggaaa tgttcatgga ataatgggaa
    26641 caactgtatt aaaggagttt gtacctacaa tggactatcc tagtgggcag ctaatattaa
    26701 gacctagaaa tgaagtaggc agagataatc ttaataagat gcttacaaat gataaaatat
    26761 tagaagaagt tccatttaca cttgcttcta ctcattttat gtttggaaaa ggttcaatca
    26821 atcaaaaggt tgggttaaat ttctttgttg attcaggtct tggtgacgaa aaaggagcag
    26881 gaattgtatt aactgaggaa ataatgaatt ccttgaaaat tcctatgcct gagctaaaga
    26941 aagcagagga aggaaaaggt ggaatggctg gaagtgatta taaagaagga tatttcaatc
    27001 tttcatctta tggtctagga gatttgcaac ttaaaaacgt ccttggatat tattttacag
    27061 gtgatacgtt ggattatgat gaggggattg gatttgttag agatggattg ataagtcatc
    27121 actatttaaa gaactataaa tggtctattg actttgattc tatgaagatg acatttagta
    27181 agtaaagata gtatataata tgaattaagt gggaaagttt caactgttca aggaaacggt
    27241 gatacaagaa aagtatcacc gtttttttta ttatacaaaa aacagttgac tttttatatg
    27301 aacccctcct ataatgttac tgaaattaag taaaatgatt gaacaaatgt aaagataatt
    27361 gaaaaagctt gattttggag ggaagcaaat gaaaaaaatt ggagacttaa gattaatgat
    27421 gaagtgttgt tcactttatt atgaagataa tttgaatcag caggaaatag caaatcaatt
    27481 gggaatatca agaccaacaa tatcaagaat attaaaagaa gcttttgaac aagggatagt
    27541 aaaaatacag atagttgatg ttttgaaaaa tgattaccaa aaaatagaga gaagtttgga
    27601 gagaaaatat aaattaaaag aagtaatagt agtagatgat aaacaggatt cactaacaca
    27661 aaaacaagaa ttagcaagag ctgtatctga atatttaacc agggtagtaa aggaaaatga
    27721 tataataggt gtttcaatag gaacaacact aaaagaaata ccaagatatg tggaaaaaag
    27781 taactgtaaa aatgttacat ttatacctct attaggaggg attggagaca atgaaataga
    27841 tattcatgca aatcaaatag cagtaagttt agcaagggcc tttggaggtg attttaaatt
    27901 attacatgct ccagctgtta tgtctgactt atctacaaaa gagaaacttt gcaaagacga
    27961 aaaaattaaa gaagtactag atttaataga taaaacaact atagcaattg tgggtatagg
    28021 aaatcctatg agtttaaact caaccataat ggcttctgga tatatgaatg aagcagatat
    28081 agaagattta aaaaaataca actcaatagg tgcaatttgt ttgcaagctt ttgataaaga
    28141 agggaaaact tctatacttg aatttaatca aagagtatta ggagtaaaac tggaagattt
    28201 aaaaaagata aaaagaacaa taggtgtcgc ttcaggagat gaaaaaatag aggcaataaa
    28261 ggcatcatta aaagcgaaat ttataaatag tttagcaatt aatcacagtt tagcacttaa
    28321 attattagaa gattgtgact aatagaaaga tagataaaag ttaacaggag ggaatatata
    28381 tgtcaatatc aaaagaaaca ttgcttgaaa tgtataagag aatgaatcag gctagaaaat
    28441 ttgaagagaa agttagttgg ttttttgcaa gaggtatggt acatggaaca actcacttat
    28501 ctgtaggtca agaagcatca agtgtggcag ctgtaatggc tttagaaaaa ggagatttag
    28561 tatcattaac tcatagaggt catagtcaat ttataggaat ggggatagac ctaaataaaa
    28621 tgatggctga actaatgggt aaagaaactg gcttttgtaa gggaaaagga ggctcaatgc
    28681 atatagcaga tattgagtca ggtaacttag gagcaaatgg agttgtaggt ggaggtctta
    28741 caatagcacc aggggctgca cttacacaac agtataaaaa aacaggcaaa atagttttat
    28801 gtagttttgg tgatggggca tcaaatgaag gtacttttca tgaaggaata aatttatcat
    28861 caatatggaa attaccaata atattttact gtgaaaataa tttatatggt atgtcaacaa
    28921 gtataaaacg tcatatgaat atagaaagta ttgcaactag agcagcttct tatggaatag
    28981 aggggatatc aatagatgga tataacccaa tagaggtata tgaaacagta caaaaagctg
    29041 ctgaaaagtg tagaagagga gaagggcctg tattaattga aagtaggact tatagatggc
    29101 taggtcattc aaaatcagat gcaaatgtgt atagaacaaa agaagaaata gagtcatgga
    29161 aagcaaagga ccctattgaa tttttaagaa attatcttat agaaaataat ttatctaatg
    29221 aagatgaatt agataagatt caagaatttg caaaacaatc aatagaagat gcagtagagt
    29281 ttgctcaaaa tagcccaaat ccaaaaatag agtctttatt agaggatgta tatgcagact
    29341 aagaatagta aaggaggaaa ataaaaatga gtacaaggga attaacatat gcacaagcaa
    29401 taaaggaagc aatgtcagaa gaaatgagaa gagatgaaaa tgtaatattt atgggagaag
    29461 atataggaat atatggagga gcatttggcg tatcagttgg tatgatagat gaatttggac
    29521 cagaaagagt gagggatact cctatatcag aagcagctat agcaggagcg gcagcaggag
    29581 cagcagcgac tggtttaaga cctattatgg aagtaatgtt tatggacttt gtaacaattt
    29641 ctatggacgc aattgtaaat caggcagcaa aaatgagata tatgtttgga ggaaaagctc
    29701 aagttccaat ggttgttaga tgtccaggtg gttctggaac tggttcagca gagcaacatt
    29761 cacaaagttt agaagcttgg ttttgccatg taccaggagt aaaagtagtt gcaccttcta
    29821 ccccagcaga tgctaaggga ttgttgaaag cagcgataag agataataat ccagtcatat
    29881 ttgttgagaa taaattgtta tacagaaaaa aaggtgttgt gccagaagat gattatgtaa
    29941 ttgaaattgg aaaggcagat ataaaaaaag agggtactga tgttacagta atcacatatg
    30001 gaagaatgtt acaaagtgtt gaagaagcag cagagaatct atcaaaagaa aatataaacg
    30061 ttgaaattat agatttaaga actttatacc cattagataa agagactata gtgaaaagtg
    30121 tatgtaaaac tggaagagtt ttaatttgtc atgaagcagc taagactggt gggttaggtg
    30181 gagaaatatc agccttgata acagaaagtg aatcatttga ttatttagat gctccagtaa
    30241 aaagaatttg tggaaaagat gtgccaatac catataatcc agagttagaa aaagcagttg
    30301 tcccaagagt tgatgaaata gaagaagcaa taaaatcttt aattgttaga taaggaggga
    30361 tagatatggt ggcaaataaa gtaaaagcca caccagcagc tagaaatcag gcaagaaaag
    30421 acaatataaa attagatagg ctgattggaa gtggagagaa tggaaggatt catttagtgg
    30481 atgtgttaaa ttatttaaaa gataacaaag caaatactac tcctttagct agaagaatag
    30541 cagaagattt aaatatagat ttagaaacta ttgtaggtac tggatataat ggaaagataa
    30601 gaaagtgtga tgtggaaaaa ttaactgaaa aagaaactat tgtatcaact aatacgtcta
    30661 aatttagtga gaaaaaagag acaaagattg aaaatgaaaa ttcaagtatg tttaatacag
    30721 tagaaggagt atttgaaaaa ccaaatccaa tgagagccac tgtagcaaga cgaatgtcag
    30781 aaagttattt tagtgctcct gtatttacat tcaatataga agtagacgct acagaattga
    30841 aggcacttag agtaaagtta atagacacag taaaagaatc aactggagta aaattaacta
    30901 tgacagattt aatagtaatg gcagtatcta gaatacttcc aaatcatcaa gcattaaact
    30961 cagcatggac agatgagggt atatttagat ataaagatat aaatatagcc attgcagttg
    31021 gtctagatga aggtttatat gttccagtag taaaaagtgt aaacaaaaag tctttaaaag
    31081 aaatagcaaa agaaagcaaa gaacttgccg aaaaagtaaa gacagggaaa cttatgccag
    31141 cagaccaaga aggtaataca tttacaataa gtaatgtcgg aatgtatggt ataactacat
    31201 ttacacctat aataaatatg ccttcaagtg caattttagg ggtaggtgct acacaagata
    31261 agtttgttcc agtaaatgga gaggctaaaa taaaacctat aatgaactta tcattgacat
    31321 cagaccacag agtaatagat ggaacagtag ccgcaaaatt cttaaaagat ttaaaagaat
    31381 tgttagaaaa tcccttatca atgcttgtat aggttttgac atttcgttac tgatataaat
    31441 agtttgaata gattatatta ttataattat ctcagtaaat ttaaaacttt tatatattga
    31501 gaattatttt taaaggtaac tgtatatgaa tatgatttgc ttaattaaaa tatatgaaaa
    31561 taaataggag gagagcctat gtcagtagaa gtaataatgc caaaagcagg tgtagccatg
    31621 gaagaaggta ctatagtatc ttggctaaag caagaaggcg aagaagtaaa gattggagaa
    31681 cctatattag aaataacaac agataaagtc aatatggaga tagagtcaga aggagaaggt
    31741 acacttgctg caatcataca taaagaggaa ggtgaagtat taccagtatt tacagtaata
    31801 ggtgtaatag cagaaaaagg agaaaatcaa gaggaagtaa aagctaaata tttatctgga
    31861 aatgtttcaa aagaagatat agttgaagag aatcaaaaca tagaagctaa agaagaaaaa
    31921 ataaacaaaa aagagtgtaa tcatgattat gatgtagtgg taataggtgg aggacctggg
    31981 ggatatttat cagcattaaa agcagctctt ttaggtggaa gagttgcttt agttgaagaa
    32041 aatatactag gaggtacttg tttaaataga ggttgtatcc ctacaaagac ttatataaaa
    32101 acggcagaaa tattagaaga aattgaccag ttatcaaaga gaggcgtaaa agtaactgta
    32161 gataaagaac aggacattaa gaaggctata aaatataaaa atagagtagt taaaaaactt
    32221 acagctggtg ttggtggact attaaaaagt agagatgtag aggtgtttaa tcttaaagct
    32281 agtgttaagg aagagcataa agtaatttta tcagatggaa aagttttaga tacagaaaat
    32341 ataataattg ctacaggttc taaggtaaga atattaccta taaaaggaat agaatcaaat
    32401 ttaataataa ctagtacaga agctttagat ttagaaacag taccagaaga attagtaata
    32461 attggtggtg gagtgattgg ttgtgaattt gcagagatat tcaattcaag aggctctaaa
    32521 gtaactatag tagaaatgga agatagagta attccaaaaa tggataaaga tttaagtgaa
    32581 tctttaaaat attctcttgg taaaaaaggt ataaacgtgc taactaaaaa gaaagtttct
    32641 gaatttaaag aagaaggaaa taacatatta gtgtgtattg aagatgaaga accaataaaa
    32701 gcagatttat gtctatatgc tataggaaga gaagctaatc tttctggtat agaggactta
    32761 gatattaaaa tagataaagg ttcaatagtt gtaaatagca aaatggaaac aagtatacca
    32821 agcatatatg cagtaggtga tgtgactgga ggtgtaatgt tagctcatgc agcttttaaa
    32881 atgggagagg tagcagcttc aaatgctcta ggtatgaata aagaagttga tttaggtgca
    32941 ttacctagtt gtgtttatac aattccagaa gtagcatcag ttggtattac agaagaggat
    33001 gctagaaaaa aatataatgt aaaagttggt aagtttaatt ttgctggaaa tggaagagcc
    33061 ttagcttctg gtcaagaaca aggatatgtg aaagtagtag cagatgctaa atatggagaa
    33121 attttaggta tacatatgtt tggatgtggt gtagcagaac ttataaatca tgcagcatca
    33181 ttcaaagcat tagagatacc tgctgatgaa gctagtgagt taatttttgg tcatccatgc
    33241 acttcagaag ctttgatgga ggctttagca gatgtaaatg gagaatgttt acacttacct
    33301 aaaaaataaa ttgttaatgt aagtctatta ttaatgttaa tttatatcat aaagttttaa
    33361 aagaattact tagccatacc ctttgttgta caaataaatt atttgcacaa caaagggtat
    33421 tttttatgga aaaatatata gtatgaaacc tttgtagttt ttgttataca aaaaagatag
    33481 taatataaaa taacagtatt attttagaat gatttcaatt gtaattagaa aaaatgattt
    33541 ttttgttttt aagaaaagta ttatttgtaa aatgtaaaaa aaattttctt aatatttcaa
    33601 taaaagaata gcttgattta aatgttatta ctttataaaa aaataataag cacaaaaaaa
    33661 acaatatatg gcatataagt tgctatataa ataataagga gggaacaatt ataaatgagg
    33721 caaagaataa aaaacttaat tgaaaatgaa gataaaaaaa atcctttaac agatgaagcg
    33781 atttcaagta tgttaaatat tcgaagagaa gatgttacct ttttaagaaa tgagttaaaa
    33841 atagaagatt caaggcaaag aagaaaagtt gttttaatta aagctataaa agacatttta
    33901 aaagaagaaa aaactataaa taaaaatgat ataacaagaa gattaaacag cattggtttt
    33961 acagtatcaa gatttacagt tatacagtac ttaaaagagc ttcaagattc tgataactta
    34021 aaactttata aagaaagtga tgataaaaaa gaagaaataa acaataatag agaaaacaca
    34081 gatatagcat ttgataaact cataggtagt agtggtagct taagtacaat tataaaacta
    34141 gctaaagcag caattctata tccacctcat ggtcttcata ctatcattat gggaccaact
    34201 ggtgttggaa agagtgagtt agcagagtgt atgtataaat ttgcactaga atcatccaga
    34261 ttccctaaga atgcaccatt tatagttttt aatgcagcag attattcaga gaatccacag
    34321 cttttgttat cacagttatt tggacatgtt aaaggtgctt ttacaggagc tgacgagagt
    34381 aaagaaggat tagtatcaaa ggcagatgga ggaatactgt ttatagatga aatacataga
    34441 ttacctcatg aaggtcaaga aatactattt caattgattg ataaggggat ggtcagaaaa
    34501 ttaggagaaa caaaactaac aaataaaata gatgtaatga ttatttcagc aacttctgag
    34561 ccaatagatt ctcatttact taatacattt aaaagacgta tacctgtaac aattgaaata
    34621 ccagaattag ttgcaagacc attaaatgag aggtttgata ttataaataa tttcttctta
    34681 atagaagctc aaagaatggg agcaaacata catataaagt cagatgtatt aaaagctctt
    34741 atgctttacg actgtatagg taatgttggt caactgagaa gtgatattca agtagcttgt
    34801 gctaggggac tactaaatca acttacaaat aaacaaaaag aagtaaacat aagcatatca
    34861 gatgtacctg gatatgttaa acaaggtctt ataaaaatca gaaattgcag aggtaaaata
    34921 gaaaattatg tagatggaga cttaattata gactcaaatc ttcaaggtga aatagaaaag
    34981 aaaaatgaag acatatatac ttttccagat gaaatatata aacttactga aagaagacat
    35041 attgatttat tggaacaagg tttagatttt gatgttataa atagaatcgt tggaggagaa
    35101 ttagaaagtt gcattcaaaa atacataaag caagttaaaa aagtttctat tagtaataat
    35161 cttccagata taagtccgat tgttggaaaa tatatagtag agctaactta tgaagtaatc
    35221 aaaatagcat cacaatatct taattcatta gaaccaaatc tacatttatg tttagcagta
    35281 cacttaaaag ccgcttttga gagattaaaa gaagggaaag taatatcaaa tgctcatttg
    35341 aaagaaataa taggaaatta tccactagaa tattctgtag cagaaaaggt ggctcaatat
    35401 ctaaaggaaa gatataacat aaatttgcca aaagaagaaa taggtataat agcaatatat
    35461 tttaaaatga gtagcagaag aaatatatct gaacaaaaaa agataggtgt acttcttatg
    35521 gctcatggaa gggtagcatc ttctgtatgt gaggttacaa ataaactatt aggaattaat
    35581 catgcaaggt atttagatat gccatttgag aagaaaccag aagaaatgat tgaagaagct
    35641 attacaataa taaaagatat tgatgaaggt agaggtgtaa ttatactagt agatatgggt
    35701 tcactttcat attttggaga tattatttct ggaaagactg ggattgatat aagaacaata
    35761 tctagatttg atacattgct ttctatagaa gttatacgaa gagcagtttt accagattta
    35821 acaatagacg agatagttga tgaagttaaa ggtatagagc cacaagtaaa agaaaaagtt
    35881 agtaaatata acaatacaaa agtaaatttt agaaaaccta taattttaac attatgtatt
    35941 actggattag ggggagcttg taagataaaa aaaatcctag aggaaaatat taaaagtata
    36001 actgaaaagg ttgatataga gcctataggt atgattgaat atccaagtct tgatttagag
    36061 ataaaacaaa taagtaatga taaaaatgta attgccatag ttggaacaat tgaccctcag
    36121 atagcaaata tccctttttt tagcctagta gatataaatg ataaagtaaa gttaaatcaa
    36181 ataaacaatc taattgatac aaatcaaatt agtgagacaa ataaaaaata tgaattatca
    36241 gatttaatac ataaagaaga tatattcgta gatttacaag ttaaatccaa agaagaagtt
    36301 ctaaaatata tgagtaaaaa acttagagag aaaggttacg tgacaaaaaa tttctataaa
    36361 aaagtagtgg aaagagataa cttatttcca actgaattat cagatgaaat agcaatacct
    36421 cacacagatg gaatagatgt aattagacca gctataagca tagctatttt aaagaatcct
    36481 attgtgtgga gtaaaaataa agtaaaaata atacttttat tagctgtaga ccaaaagtgt
    36541 tttaatccat taagcactct actttcattt atagaaacag atgaatttaa gcagataaaa
    36601 aacataaaag atagagattt tattagggag atgatattag atggagtcaa tgaaaataat
    36661 tagtagtaat ttaattttta aaaatattga agtgtctaac aatgaagatg cattaaagtt
    36721 tttgggacaa cgtttatttg atgaacagta tgtaaaggca agttacatac aagctgttat
    36781 agcaagagag aaaaagtatg ctacaggatt accaacagaa atatatggag tggcaatacc
    36841 acatacagat atagtacatg taaatgagcc aggaatagca attgggatac tgaataaacc
    36901 tgtaaaattt ataatgatgg gtacagatga tacagagatt gatgtaaaag ttgtatttat
    36961 gttagccgtg aaagagccac aagaacaatt gcaactatta gaaaaactta tgactatatt
    37021 tcaagataag aatatactaa ataatattgt tgacttaagc gaagaaagtg tgagtgattt
    37081 attaaactct aaattgaaaa attgaatata taaagtatat taaaaaataa aaatgttatt
    37141 gaagatttct aactataggt atctttaaaa taaaataatt tcaatggggg tgactaatgt
    37201 gactaaaaag atattagttg catgtggaac aggagtatgt acttcaacaa ttgctataaa
    37261 caaattaaag aaagcgcttc aagatattgg taaattggat atggttaata ttacacaatg
    37321 taaagtggta gaagttgcat caaaagcacc tgattatgat ttaataattt gtactacaca
    37381 agtatctagt agtataaaaa caccagtgat aaatggatta ccttttttaa ctggtgtcgg
    37441 aatggataaa ttaatcaagg atgtattaga aaagctagaa ttataatcaa aaaaattaag
    37501 gaacatggtc tctaaaatta taagttttag agacttaaaa ttaaataatg gagggtcttt
    37561 atatggagat attaaatttc attgttggtc taggcgcaca agtaatgatg ccgataataa
    37621 tatgtatttt tggtttgata ttaggtacaa aactaggaaa atctttaaga gctggtttaa
    37681 ctgtaggagt tgggtttata ggtcttaata ctattatagc tttgctaaca gataatttag
    37741 gtccagctac acaacaaatg gttaagaatt taggactaag tttatccata atagatgtag
    37801 gatggccagc agcatcggcc atagcttttg catcaacagt aggggcttta attattccaa
    37861 taggtcttgt ggtaaatata attatgttaa ttactaatac cactcaaact gttgatgtag
    37921 atatatggga ctactggcat tttgctttta caggagcttt agtggcaggg gctactcaaa
    37981 gtgtaatgtg gggagtattt gcagcagtag caaatatgat aattgtattg gtaatggcag
    38041 atttaactgc tccagggata gaggaatatt taggtatgcc aggtatatct cttcctcatg
    38101 gatttacaca agcatttgtt ccaattgcta tagtagtaaa taaattatta gacctaatac
    38161 caggcataaa taagattgag ataaatgctg atacattgca gaaaaagttt ggattatttg
    38221 gagaaccttt aataatgggg tctgtaatag gggtaataat tggtgttgca gcgaaatatg
    38281 atataaaagg aatattacaa ttaggtgtaa ctatgggagc agtattaata ttaattccaa
    38341 agatggcagc gttattaatg gaaggattat taccagtatc agaatctgca caggaattta
    38401 ttgaaaagag atttaaaaat agaggtaaga tttacatagg cttagattca gcagtaggaa
    38461 taggtcatcc tgttacacta tcagttgcat tagtgttggt tcctttgact atacttatag
    38521 cagctatatt gccaggaaat aaagttttgc catttgcaga tttagcagta attccatttg
    38581 cacttgtatt aattgtgcca attacaaagg gaaatgtgtt tagaacatta ataataggaa
    38641 taattataat tacttctggg ttattaatcg caactaattt agctccatta tttactcaaa
    38701 tggctttaaa tgctagtttt aaaatgcctg aaggagcaac tatgatttca agtatttgtg
    38761 atggtgcgaa tccattaagt tgggtatttg taaaagttat gaactataaa gttattggag
    38821 gagtagtttt tggagtcata gctttaggaa tggctattta caataggaat agaataataa
    38881 atgaaaataa aaagctattt atagaagaat aaggttaaaa caattttatt aaggagtgta
    38941 taagttatga aaaaatataa atatgattct aaaaggtact tacttagcgt tacaatacca
    39001 ggatttttta tgattttaat attgatttat gcactgttta ataactatat aaattttaat
    39061 ttgaatatat atagtttttt aataattgtt tgtacttatg gtatattcaa tactttcata
    39121 tcatcaagta atcctaaaaa aattattatt gataacaaat gtatacattt ttcttcattt
    39181 atgactacac ataagtatga aattaaagat atagaaaaga taagaataag agaattttat
    39241 aataaaaaaa tttatctaaa aattaatgaa ggaaaccttt ttaaaggtag gtattggata
    39301 agaacaaata tgtttaataa tagtatagaa ttatatagct atttaattga gttggaagag
    39361 tgcttatatc cagattcatt aaagtttaga aataaaagat ttgagaataa gaacatatag
    39421 aaataaaaaa attaaattga agcctagttt aatatgtttt gatagaatat gagtttgagc
    39481 ttcatgctga atttttagaa ttttcaatta aaataataat ataatgtcta agcaattaga
    39541 gacgtataag gcatgtatat aatgaatggg aggatttaat taatgttatt agacaaatta
    39601 agaaaagaag tattacaagc atctttagac cttttaaatt ataatttagt gacattaaca
    39661 ggaggaaatg taagtggtag agatgaacaa actggctata tagcaatcac cccaagtgga
    39721 atggattata gaaatcttat accaagtgat attgttatag tagatgtaga tggaaatgtt
    39781 atagatggaa aatggaaagc atcagtagat ttaccagacc acttgtatat atataaacat
    39841 agagaagaca taaatagtat aattcataca cattctacat actcaagttg ttttgctatt
    39901 ttaaatgaac ctatagaatg tgcaagtaca actcttgcaa atgaagtagg aggttcagta
    39961 ccagttgcaa aattttcacc accaacgtct aataagatgg gaaaatgtgt tattgaagca
    40021 attggtgaca aaagagcatg tcttttagca aatcatggtg taattgcagt tggtccatct
    40081 gtaggacacg ctcttacagc tgctgtaatg ttagaagatt cagcaaaagt atattactta
    40141 gcaaaatcaa ttggcacacc agtgctactg cctgatgaag aaattcaaag agctagagat
    40201 gtatttttta atgtctatgg tcaggataaa taaacgagat aaaattttaa aagtaatatt
    40261 aaaatataga tattcaatga tattaaggag atttataatg atataataag tttaatagtt
    40321 aagaagtaaa atatgaaatg taatcgtata taaattcact taagactaac aaataataag
    40381 ataaaataaa aaaggagtct gtatttctat gtattactta ttaaacaaaa gcacaaatcc
    40441 ttactttaat ttagcattag aagaatatct gtttttaaat gataaatata atgatgatat
    40501 tataatcata tggaggaatg aagaatccat ttttataggt aaaaatcaaa atccatatca
    40561 ggaagtacat catgatgtta ttgaaaaggg cgaaattcct attttaagaa gaatatctgg
    40621 aggaggcact gtttatcatg atttaggtaa tattaatatg tcattcatac aaaaagatag
    40681 acaacttcat gagatagatt ttttggagca tactaagttt atgcaaaaga tgctcttaac
    40741 cttgggatta ggtgtatcta taactgaaag aaaggattta tttttaaatg ggaaaaaaat
    40801 ttcaggctct gcacagtcta taaaaagaaa aaattctctt tatcatggaa ctcttcttta
    40861 cgattcagat ttaaataaat tgactaagta tttaaactca gataaatcaa ctgaatctaa
    40921 tgcaactaag tctgtttcaa gtaaagttac aaatattaag tttttgctcg aaaaaaatat
    40981 aagttatttt ttagattatt gtgttgaata tttaaaatct aatataaaaa atataaaaga
    41041 acttgaatta gatgaagaag atattaaaaa tgtatacaat ttagtagaag ataagtataa
    41101 aaagatagag tggacatatg gtaaaacgcc taaatttcaa gtggtgctac catttaataa
    41161 taaaagtaaa attaatatac atgttaatag atggaaagta tcaaaatttt ttatttctta
    41221 tgatgataat gtaattaata tggacaagtt gttggattta aatttcttta aagaagaaat
    41281 aaaagagagt attttagaaa attatccaca ttacatagat ttaattaatt taatatttta
    41341 atataaaacc tctaatttga taaaattgtc ttatatcaaa ctagaggttt ttaatatttt
    41401 tacatatcaa taaccattaa aaaattgtaa aattaaaaac aatactattg aaaaatcaat
    41461 atatattgta ataaatatac atgtttttca ttagtaaata gacattagct atttatacaa
    41521 ccgaaaattt aatatataat taatcagatg gttaaaataa aaaatattaa aagttttaat
    41581 aaatgatggt taaaaataaa atacgaggtg ataccatgta tagtgttata attgttgcgg
    41641 caggaagtgg aaggagaatg cacttagaca taaacaaaca gttcataaaa ttaagagaaa
    41701 aagaaattat tgctcataca attcaagttt tttatgaaaa tataaatata gatgaaatag
    41761 tggtatgtat aaagaaagaa gaagaagatt tttttaagga aaacataata aataaatata
    41821 attttaaaaa cataaaaata gcatatggtg gaaaagaaag acaggattca atatataatg
    41881 gtttaaaaga attagataaa aattgtgata tagtacttat acatgatgga gcaaggccat
    41941 ttgtagacca tagaataatc aacgaatcaa taaaggtagc taaagaaaaa aaggctgtgg
    42001 ttgtaggtgt tccagtaagt gatactataa agatagtatc agatggaaca gtacaagaaa
    42061 ctccagaaag aagtttgctg tgggcagcac aaacccctca aacatttgaa tataatctca
    42121 taatagatgc ttatgaacaa gcatataaaa ataattatta tggtactgat gattcaatgt
    42181 tagtagaaaa tataggtcaa agtgtcacta tggtaatggg ttcatatgaa aatataaaaa
    42241 taactagtcc agaagattta aatatagcag aacaaatatt aaatatggaa aaaagagatg
    42301 aagttaatag tagaaggaga attatatgag aataggatta ggatatgatg tacacaaatt
    42361 aacagaggat agaaagctta taatcggcgg tgtagagata ccgcacgata aaggtttgtt
    42421 ggggcattct gatgctgatg tattgataca tgcaataatg gattctattt taggtgctct
    42481 agcattagga gatataggga aacatttccc agatacagat gaagaatata agggagcaga
    42541 tagtatgaaa ttattagaac atgtgtataa tctaataaca agtaaaggat ataaaattgg
    42601 aaatatagat agtactataa ttgctcaaag tcctaagatg gctccataca ttgagaatat
    42661 gagaagtaat atatcaaaag ttttaaacac agacattgat aatataaaca taaaggcaac
    42721 aacagaagaa ggtcttggtt ttacaggagc aaaacaggga atagcttcac aaagtatttg
    42781 tttattattg ttgacaagtc agaataatta atatattatt agtaataagt ttaatggata
    42841 tcaagtaata ggcctatata gaatatctat aatgtgtgaa tttaagtgaa attaatataa
    42901 atataaaatt agctatgata aagaaatagt aaatgatgaa atcttcacag agaggaaacg
    42961 gtggtgagag ttttcaagag gaagtctttg aacctgtctt ttagcttaaa ggtataaaaa
    43021 tctttacggt aggaaccgtt atattcccaa aagagagcta ttcatatagc taactagagt
    43081 ggtaccgcgg aaattaaacc tttcgtctct atacttgtat agagatgaga ggttttttat
    43141 attttcagaa agttaatatt caggaggaat ctattaatga aaatgtcaaa aatgtttatg
    43201 ccgacattaa aagaagtccc agcagatgct gagattacaa gccatcaatt aatggttagg
    43261 tcaggcatga taaaaaaaat gacttcaggt gtttataacc aattaccaat ggggttaaga
    43321 gtgttcaaaa aaattgaaca aataattaga gaagaattaa ataaaaaaga ttgtcaagaa
    43381 atactttgtg cagccttact tccttctgaa ttatggaaag agtctggaag gtggactgct
    43441 atgggtgaag aaatgtttag attaaaagac agaacagaaa gagagtattg tcttgggcca
    43501 acacatgaag aagcattcac tgacataata agacaagaaa ttacttcata taaacaattg
    43561 ccactgaact tgtaccaaat acaagtaaaa tatagagatg agagaagacc aagatttggt
    43621 gttatgagaa ctaagacttt tacgatgaag gatgcttata gttttgatgt agatgataaa
    43681 ggtctagata agtcatatca agatatgttt gatgcatatg taagtatatt tgatagatgt
    43741 ggattggaga atagccctgt acaggcagat tcaggagcta taggaggttc tacatcaact
    43801 gaatttatgg ttaagtcaga agttggtgaa gatgaagttg tattttgtag tggatgtgac
    43861 tatgcagcca atgtagaaag agcagaatct tgtaatgtag catcagaaaa agaagaaatg
    43921 aaagaattag aagaggttta tacaccagga gcagctacaa taaaagagct agaagaattt
    43981 ttaaaaactt ctccagataa gtttgctaaa actttagtat atgaggcaga tggaaaaaca
    44041 gttgtagtcg ttgtaagagg tgatagggaa gttaatgaaa tcaaagtatc taatgctatt
    44101 ggctctgtta tagaatttgc acttgctaca gatgatgtag tgagaaaggt gactaatgca
    44161 gaagtaggat ttgcaggacc aataggtata aatgctgatt atgtatttat agataaagaa
    44221 attgttgaac aaagaaacat agttgttggg gctaataaaa ctgagtacca tataaaaaat
    44281 gctaactatg gaagagattt tgaaggagta gttggagact ttagaaatgt tcaagaaggc
    44341 gacaaatgta tagtatgtgg taagccactt gaaatagcta ggggtgttga agtaggacat
    44401 atatttaaac taggaactaa gtactcagaa tctatgaatg caaacttcat agacagagat
    44461 ggaaaatcaa agcctatagt aatggggtgt tatggaatag gtgttgaaag aacagcagca
    44521 gctataattg aacaacataa tgatgagaaa ggtataatat ggcctttatc agtagctcca
    44581 taccatgttg tgatagttcc agcaaatatg aagaatgaag aacaaatatc aatagctgaa
    44641 aaaatatata atgatttaca ggctatgggt gtagaagttt tattagatga cagagatgaa
    44701 agaataggtg tcaaattcaa tgattcagaa ttaataggaa tacctatgag aattacagta
    44761 ggtaaaaata ttaatgaagg taaagtagaa tttaaattta gacataaaga agataaagag
    44821 ataattgaca tagaagaaat aaatgaaaaa gtaaaggcag agtttatcag aaataatgtt
    44881 aggttaggat aataacttaa ataagatatt gtatagataa tcagagttgt actttataga
    44941 caccatttta tctatatata aggatataaa atggtgtttt tttataaaac aaataaatga
    45001 ggaggtaatt gaaatggcaa aaaatgaaaa acaatttgtt gaagaaataa caaagatgga
    45061 agatgatttt ccacaatggt acacagatgt aataactaag actgacttag ttgattatgc
    45121 accagtaaaa ggttttatgg ttgtaaagcc atatggatat gcattatggg aaaagatgca
    45181 ggaatttatg gataaaaaat ttaaagaaac tggacacaaa aactgttatt ttccattatt
    45241 aataccagag agtttattaa acaaagaagc agaacacgtt gaaggttttg ctccagaagt
    45301 tgcatgggta acacatggag gaaataaaaa attagaagaa agattatgtg taagacctac
    45361 ttcagagact ataatatgta caatgtatgc gaagtggtta aaatcatata gagaattacc
    45421 atacctttat aatcaatggt gctcagttgt tagatgggaa aaatcaacta gacctttctt
    45481 aagaacatct gagtttttat ggcaagaagg tcatacatta catgaaacag cagaagaagc
    45541 acaagaagaa acaatacaac aattagaagt atataaagca ctatgtgaag aattacttgc
    45601 aatgccagtt gtagctggtc aaaaaagtga aagtgagaaa tttgcaggtg gtgaaagaac
    45661 atatactata gaagctatga tgcatgatgg taaagcatta caatcaggta caagtcactt
    45721 cttaggtcaa cactttacta aggcttttga tattactttt gcagatagag aaggaaactt
    45781 agctaatcca tatcatacat cttggggagc atcaacaaga cttataggtg gacttataat
    45841 gactcatagt gataacagag gtttagtatt accaccaaga gtagctccaa ttcaagttgt
    45901 tatagttcct atagctgcca aaaaaggaaa tgtaatggaa actgttgata aaatatatgc
    45961 tgatttaaaa gctaagggag tagctgtaga agttgatgat agagataact atacaactgg
    46021 atggaaattt aatgagtggg aaatgaaggg tgttccagta agaattgaaa ttggaccaaa
    46081 agatatagaa aacaatcaag ctatggtatt tagaagagat actttagaaa aggattctat
    46141 gcctttagaa ggattagctg atgctgtatg tgatttattt gatgttatac acaatgatat
    46201 gtttgaaaaa gcaagaaaac atagagaaga caatacatct atagttgaaa atatggatga
    46261 gtttagaaaa gctttagaag aaaagccagg attcataaag actatgtggt gtggagatgc
    46321 agaatgtgaa gctaagatta aggaagaaac aggagcaact ataagatgct taccttttga
    46381 acaagaaaac ttaggtcaca agtgtgtgta ctgtggaaaa gaagcagata gtatggtagt
    46441 aatggcaaaa gcttattaaa ataatacatt taaataaaaa ctggggtaga aagtatttaa
    46501 tactttatcc cagttttttt attttttatg agaaataaaa tcgtgtttgt agatgtatca
    46561 taagataagt cataaatgct tatattaaaa agaatataat agagaactat ttttaataat
    46621 tacatattaa tgaaagaaaa atatgtttga tatgtaaatt ttcaataaag taaacaataa
    46681 atataattga atattaataa agagtacttt taatttttag aaaataaatg acttaaaaga
    46741 aagatttatg aaattaaaac taggattttc tgatataata aacatgtgta tttttaaata
    46801 aaaaggatat tattattaga tttgattaaa aataaacttg caaaacaatg tactagttta
    46861 atattatagt tatattgaat aaaatatcag gaggaagtaa aaatgaatgt aagagtaagg
    46921 tttgcaccaa gtccaacggg atttgtacat ataggtagtt taagaactgc tttatataac
    46981 tatttatttg caaaaaaaat gggcggagaa tatatattaa gagtggaaga tacagaccaa
    47041 agtagattag tagaaggtgc aatagagaat atgcttaatg ctatgaaatg ggctggtgtc
    47101 aaccatgatg aaggtgttgt attagatgat agtggaaaag tggtacaaaa aggagaatat
    47161 ggtccatata ttcaatctca aagattagat atataccaag aatatataaa gcaacttttg
    47221 gatagtggaa aggcttatta ctgtttctgt acaaaagaaa gactagatga agtaagagat
    47281 gctcaaagag cagcaggaga aacagctaaa tatgatggtc attgtaaaaa tctttccaaa
    47341 gaagaagtag aagcaaatat aaaagcagga ataccatatg ttataagatt aagattgcca
    47401 gaaaatcata caattaaatt tacagattta gttagaggag atatggaatt taacactaat
    47461 gacttagatg accaagtatt aatgaaaaca gatggatttc caacttatca ttttgcagta
    47521 gttgttgatg actatttgat gaaaattaca catgttataa gaggagaaga atgggtatca
    47581 tctactccaa aacatgttta tttatatgaa gcttttggat gggaagcacc tgtatttgtg
    47641 catttaccaa atatacttaa taaagaaaag aaaaaattaa gtaaaagaca aggtgatgta
    47701 gcagttgaag actttaagaa aaaaggatat ttaccagaag gattagtaaa ctatgtagca
    47761 ttagttggtt ggtctccaga agataatcaa gaattgttta caatggaaga attagaaaaa
    47821 gccttctcta tagaaagagt atctaaaagt ggtggagtat ttgatactga aaagctaaat
    47881 tgggttaatc agcattatat aaaagatggt gatgatgctt acctaacaga cttagcaata
    47941 ccatttttaa tagaagatgg atttataaca gaagaagagg ctactaataa atatgatttc
    48001 ttaaagagta tgatatcagt acttaaagag aaattacaat atgtaaaaga ggttactgag
    48061 catgcaagta tattttttgg agataaaata gaggtagaaa ctgaagaggg aagagagttc
    48121 ttaagattag aacatatacc tactttaata gatgctttga aagaaaaaat agaaaaagca
    48181 gaagtattaa atgctgagtt cgttcaagct atgcttaaag aaatacaaaa agagtacaaa
    48241 ataaaaggaa aaaatttatt tatgggctca agaataattt taacagggca aatgcatggt
    48301 ccagatttac caaaagtaat ggaagtttta ggaaaagaaa cttgcttaaa tagaatagct
    48361 tatgtaaaaa ataatatatt atagtcataa aagtccgcta atttagaagc ggactttttt
    48421 cgtgttagta tttggtgaaa atatctgctt aagaaaatga aggtatttaa ggtaataaat
    48481 ttaatatata aaaagcaaag caaaaatata aataaaatat gaataggaaa ctaataaaat
    48541 attaagcata gaagaaggga atctattatt ttagtttgat ttaattatta agggggtaat
    48601 tattatgtat agtaatattg atgatgttaa aaaggaatta aaggagctat gcttagaata
    48661 tgttacaatt ttagaaaaat taaaagatga aaaaatgatt acagaagaaa cttttgaaaa
    48721 gtgctcttct caaaaaaaga tatttttaga agaacaatag aggatagtaa tagtattatt
    48781 attatgtagt ttgaaagata gtacgaaaaa tgatagtaat actattattt attgcaattt
    48841 tcaactataa tataatatat aatgttaaga agataattta taaaaataca gtgaggtgat
    48901 gagtgtgaaa gtttataata cattgactag aacaaaagaa gaatttgttc cattagaaga
    48961 aggaaaggta aaaatgtatg tgtgtggtcc aactgtttat aactatatac acataggtaa
    49021 tgcaagacca tttataatat ttgatactct aagaaggtat ttagagtata gaggatacga
    49081 tgtaacttat gtacaaaact ttacagatgt tgatgataag attataaata gaagtcatga
    49141 ggaaggaata tctcctgaag aagttgctgc taagtatata aaagaatatt ttgtagactg
    49201 tgatgggcta ggaattaaaa gagctactgt tcatcctcag gttacagata atatacaaca
    49261 aattatagag tttataaaag aattggaaga taagggatat gcatatgcag taaatggtga
    49321 tgtatatttt gatacaaata aatttgaagg atatggaaag ctttcaggtc aaaaacaaga
    49381 agatttagaa gcaggggcta gaattgaggt taatgaccaa aaaagacatc ctatggattt
    49441 tgttctttgg aaagcaaaaa aagaaggtga gccaggatgg gatagtcctt ggggagaagg
    49501 tagaccagga tggcatatag aatgttctgt tatgtctaag agatatttag gtgagacaat
    49561 tgatatacat gcaggtggtc aggatttaac attcccacat catgaaaatg aaatagctca
    49621 aagtgaagct agaagtggaa aaacattctc aaaatactgg atgcataatg gatatataaa
    49681 tataaatgat gaaaagatga gtaaatcaaa aggtaatttc tttacagtaa gagatatatc
    49741 aaaactatat gatttagaga tagtaagatt tttcatgtta tcagcacatt atagaaatcc
    49801 agtaaacttt agtgatgaga tgttaaatca agctaaagca ggtcttgaaa ggttatataa
    49861 tactaaagaa aaattagagt ttacgttaag taatttagta gaatctcctt taacagaaaa
    49921 agaagtagaa cttgttaaag aattagatgg atttagacaa aagttcatag atgctatgga
    49981 tgatgatgtt aatacagcag atgcagtaag tgtaatattt gaattagcta agttaataaa
    50041 ttctaatgta gatgaaaatt cttctctgga gtttgctaaa aaatgcttag atgaatttaa
    50101 tgaacttaca ggagtattaa atattgtaaa taagaaaaag gatactgtat tagataaaga
    50161 cattgaagaa ttgatacaaa aaagaactga tgcaaagaaa aacaaagagt ttcaattagc
    50221 tgatgatatt agacaacaat tactagatat gggaattgta ttagaagaca caagacaagg
    50281 tgttaagtgg aaaaggatat aaaaatggaa aaaaccgaat tagttactat gtcaccacta
    50341 gttttagcat acttaggtga tacagtttat gaaacatata taagagaaca tttaattaga
    50401 cagaatacac aaaaaaaagt taatgatttg cataaattag ctataaaata tgtaaaagct
    50461 aaagctcagg caacaataat tcatgaaatt gaaattgaat taactgagga agagagtaaa
    50521 atttataaaa ggggaagaaa tcaaaagtca aatacttctc ctaagaatgc agatattata
    50581 gactataaac atgcaactgg gtttgaagcg ctagtgggat atttgtattt aaataatgaa
    50641 atagagagac ttcaatatat tatcaataaa ggaatcaaaa ttattgaaag agatatgtaa
    50701 atattaaaga gtgttgtctt aataataagt aataaggcaa cacttttttt ataactaatg
    50761 tataataaat atatagaaat tagtaatgag aaaggataaa aaattatgaa agtaaaatta
    50821 atatcacata cacctgaacc agaaaaagta atagctatgg cagctaagct atgttattca
    50881 ccagttggga cagacgaaat agaaaaagac ttaacagatg aaagtataga gaaattttta
    50941 aatatgttat taagtatagg tcatgggtca atattagaac atgcatcatt tacattttca
    51001 atagaaggta tttcaagagc ttgttcacat caaattgtta gacatcgtat agctagtttt
    51061 tcacaacaaa gtcaaagata tgttaaatta gaacaatttg agtacataat tccgccagaa
    51121 atagaaaaaa tagaaaaggc aaaagagtta tttatagact ctatgaaaaa agaccaagag
    51181 aattatgata agttagttga aatattgttt gaaaatcatt ataatgacct gataaaaaac
    51241 ggtaaaaatg agaaaacagc taaaagacaa gctgaaaaga aagcaataga agatgctaga
    51301 tacgtatttc caaatgcatg tgaaacaaaa atggtattta ctataaatgc tagaagtttg
    51361 tttaatttct ttgaacatag atgctgtgaa agagcacagt gggaaataag aaatttagct
    51421 gtggagatgt taagagaggt taaaaaggtt gctcctatat tatttaaaaa gacgggtcca
    51481 agttgtgtaa atggaagttg tccagaagga actatgacct gtggagatat agttcaagtt
    51541 agagaaaaat ttaaggcctt gtaggaggtg aatagtttgg caaatataga aggaagaaat
    51601 ccagtaatag aagcaataaa aagtgacaga gaaatagata aaatattaat tgcaaattca
    51661 gctaaagaag gttcaattaa gaaaataata ggaatggcta aagataagaa tataattatt
    51721 cagtatgttg ataaacataa gttagatgaa gtaagtacaa gtcattcgca tcaaggtgta
    51781 atagcttatg caagtgagca taagtattat gaattagatg agctaataga tttggtaaag
    51841 aataaagatg aagacccatt tttcattata ttagatgaga taacagaccc acataatttg
    51901 ggttcaataa taaggaccgc agatgcagta ggggcgcatg gagttatcat tccaaaaaga
    51961 aggtctgtac acataactcc tgtagttgcc aaagcatctg ctggagcagt agaatatatg
    52021 ccagtttgta aggtaaccaa tatagttaat acaataaaaa ggctcaagga agaagggttg
    52081 tggattgctg cagctgatat ggatggagaa actttttata aacaaaatct tactggtcct
    52141 cttggtgtag taattggaag tgaaggtttt ggaatctcta gattggttaa acaaaactgt
    52201 gactttatag taaaaatgcc aatgataggt aatgttacat ctttaaatgc atctgtagca
    52261 ggtggaatac ttttatatga gatattcaga caaagactgg gtaaaagtaa ataggtatga
    52321 aaaaaaatat aaatcattac ttaataatag atggctacaa tattataaat gcgtgggaca
    52381 atttgaaaga actggctaaa gaggacttag aagattctcg agaaaagctt attgatgata
    52441 ttatagaatt ttctgagttt atgggttata agactataat agtttttgat gcttataatg
    52501 ttaaaaattc tcgagaaaaa gttgagaaaa gaaaacacat aacaatagtt tatacaagag
    52561 aacaccaaac agcagatagt tatatagaaa aatttataac ttctttatca aagtatgatg
    52621 atgtaaaagt agccactaac gactatgcag aacagcaaat gattttagga aaaggtgcta
    52681 ctagaatgtc tgcaagagaa ctaaagttag agttagatcg ttccaagaat aaaatgaagg
    52741 aaaaaaatat cggtttgagg aaaaaaattc agcgaaattg gttggaggaa aggttagata
    52801 aagaaacatt gtcgaaactt gagaacattc gtagaaagcg ttgaaattac taggttcttt
    52861 agactataat atacctataa atttgtttta tatatggggg agatttacat gttggtagca
    52921 aaagaaaaaa gttatgagtt agtggacaat tgtcagcagg atgagtataa cattgtatta
    52981 agagcaagtg aaggggataa aatagcacta gagtacatta ttactaaata cagaaatttt
    53041 gtaaaggcaa aagcgaagtc atatttttta attggcgcag ataaagaaga tataatacaa
    53101 gaaggaatga taggccttta taaggcagtt agagattttg atggaagtaa gaccaattca
    53161 tttaaatgtt ttgcagaaat atgtataaca agacaaataa taactgctat aaaaactgca
    53221 acaagacaaa aacatatacc tttaaattca tatgtttctt tgaataaacc aatatatgat
    53281 gaagagtcag acagaacact tttagatatt atagctacaa gtatagtaac agatccagaa
    53341 gagcttataa ttagtaaaga agagttaaaa aatatagaat caaaaatgaa cgaattgtta
    53401 agtgacttgg aattagaagt actagaatta tatttaaatg gaaagtcata ccagtttata
    53461 gcggacaagc ttaaaagaga tgtaaagtca atagataacg ctttacaaag ggttaagaga
    53521 aaattagaaa aacatcttga aaacagaaat gattaatagt aaaatactta ccataagctt
    53581 attgtgagca aaattaattt atatcgaaag ataagggagg aaattcaaaa tggctaaagc
    53641 taaatacgaa agaacaaaac ctcatgttaa tatagggaca ataggacacg tagaccacgg
    53701 taaaactaca ttaacagcag caataacaaa aacattatat gacagatatc aattaggaga
    53761 agcagtagat ttcgcaaaca tagataaagc tccagaagaa agagaaagag gaatcacaat
    53821 atcaacagca cacgttgagt atgaaacacc aaatagacac tatgcacacg ttgactgccc
    53881 agggcatgct gactacgtta agaacatgat aacaggagca gcacaaatgg acggagcaat
    53941 attagtttgt tcagcaacag atggaccaat gccacaaaca agagagcata tactattatc
    54001 aagacaagtt ggagtaccat atatagtagt attcttaaat aaatgtgaca tggtagatga
    54061 tgaagagtta ttagagttag tagagatgga agtaagagat ttattaacag agtatgattt
    54121 cccaggagat gacactccaa tagtaagagg atcagcgtta atggcattag aagatccaaa
    54181 gagtgagtgg ggagataaga tagtagaatt attcgagcaa atagatgaat atataccagc
    54241 accagagaga gatacagata a
//
LOCUS       DAMJVM010000016        54171 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.016, whole genome shotgun sequence.
ACCESSION   DAMJVM010000016 DAMJVM010000000
VERSION     DAMJVM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 54171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54171
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.016"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            211..456
                     /locus_tag="PC523_001024"
     CDS             211..456
                     /locus_tag="PC523_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HDF2474569.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGIKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            491..1216
                     /locus_tag="PC523_001025"
     CDS             491..1216
                     /locus_tag="PC523_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDF2474570.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDVNPQLYGEETREETTFIYPEVDEFDLIAINI
                     ALGLQKPILGICRGLQILNVSLGGTLYQDNNLKEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDEKFVVGVQWHPE
                     MMVDYCNKMEKLFKYFINICSNL"
     gene            1239..2552
                     /locus_tag="PC523_001026"
     CDS             1239..2552
                     /locus_tag="PC523_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2474571.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKAFGVASIFVY
                     MFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTTSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARVCFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVYGENLKKIV"
     gene            2758..3297
                     /locus_tag="PC523_001027"
     CDS             2758..3297
                     /locus_tag="PC523_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2474572.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQNIIDFYNEVGGETHFLSFGKD
                     EYKISLKEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            3386..5371
                     /locus_tag="PC523_001028"
     CDS             3386..5371
                     /locus_tag="PC523_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HDF2474573.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKINDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTITLKGKTYKLKDTYLPTIDKKDPYKLTIEEKNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDRMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKKLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            5597..6565
                     /gene="spoIIIAA"
                     /locus_tag="PC523_001029"
     CDS             5597..6565
                     /gene="spoIIIAA"
                     /locus_tag="PC523_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HDF2474574.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKISNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMALDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKVIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIDD
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            6555..7076
                     /locus_tag="PC523_001030"
     CDS             6555..7076
                     /locus_tag="PC523_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HDF2474575.1"
                     /translation="MQIKIIIIALLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGRNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            7093..7290
                     /gene="spoIIIAC"
                     /locus_tag="PC523_001031"
     CDS             7093..7290
                     /gene="spoIIIAC"
                     /locus_tag="PC523_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HDF2474576.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            7306..7692
                     /gene="spoIIIAD"
                     /locus_tag="PC523_001032"
     CDS             7306..7692
                     /gene="spoIIIAD"
                     /locus_tag="PC523_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HDF2474577.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSVQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            7777..8982
                     /gene="spoIIIAE"
                     /locus_tag="PC523_001033"
     CDS             7777..8982
                     /gene="spoIIIAE"
                     /locus_tag="PC523_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HDF2474578.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSENKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLVGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTIYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            9041..9658
                     /locus_tag="PC523_001034"
     CDS             9041..9658
                     /locus_tag="PC523_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HDF2474579.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGSYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNNAKSNSSANEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLSLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            9670..10224
                     /locus_tag="PC523_001035"
     CDS             9670..10224
                     /locus_tag="PC523_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HDF2474580.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKNDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            10251..10937
                     /locus_tag="PC523_001036"
     CDS             10251..10937
                     /locus_tag="PC523_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HDF2474581.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDDSDSSKTEDSNNVDKQDGKESADIDIVDSKTSKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNNK"
     gene            11047..11409
                     /locus_tag="PC523_001037"
     CDS             11047..11409
                     /locus_tag="PC523_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDF2474582.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            11494..12000
                     /gene="nusB"
                     /locus_tag="PC523_001038"
     CDS             11494..12000
                     /gene="nusB"
                     /locus_tag="PC523_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDF2474583.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDRVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            11984..13054
                     /locus_tag="PC523_001039"
     CDS             11984..13054
                     /locus_tag="PC523_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HDF2474584.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVMDNSK
                     GLRQSEAVFQHVNNLGILSDRIKSFKDRFHVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     RLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNRFISVHMSGGTTEILLTSKQDSHH
                     NVCDIDLSKFAKTSIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGVKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIIKDNGDNANQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVSEVVFAGGVSASKYIFRELTMKLGKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            13051..14256
                     /locus_tag="PC523_001040"
     CDS             13051..14256
                     /locus_tag="PC523_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDF2474585.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGIKVIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIRRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RLSKSLTNQIELDQYRLEAVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLRLE
                     DEKLVKIGALLHNLSPLATMDRGYSIAQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IEKIENKEV"
     gene            14258..14473
                     /gene="xseB"
                     /locus_tag="PC523_001041"
     CDS             14258..14473
                     /gene="xseB"
                     /locus_tag="PC523_001041"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDF2474586.1"
                     /translation="MNLTYEEAYKRLESILNELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            14476..15363
                     /locus_tag="PC523_001042"
     CDS             14476..15363
                     /locus_tag="PC523_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDF2474587.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDESKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLNGLAEYIIDREY"
     gene            15393..15830
                     /locus_tag="PC523_001043"
     CDS             15393..15830
                     /locus_tag="PC523_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HDF2474588.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGVVTALILM"
     gene            15864..17729
                     /locus_tag="PC523_001044"
     CDS             15864..17729
                     /locus_tag="PC523_001044"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDF2474589.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KESESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYINTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKDLIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSGKVKSMSTSVGEKLVDMANNNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     RELELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDNGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEDMLKVLLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNADELYDFVGLSPKSIADKIRKLFIK"
     gene            17862..18674
                     /locus_tag="PC523_001045"
     CDS             17862..18674
                     /locus_tag="PC523_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HDF2474590.1"
                     /translation="MKKRIDLLLVEQGHFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCNIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEIVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNDGYEFDGEIYNNKIVEVVEASHNLEK"
     gene            18694..20373
                     /gene="recN"
                     /locus_tag="PC523_001046"
     CDS             18694..20373
                     /gene="recN"
                     /locus_tag="PC523_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDF2474591.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNTYLKKILEENDISIEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYRKIK
                     DRLDEILNRDERVEQLKSKLMKIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGLFTLDGVDDIEFMISFNLGEDINPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            20588..21454
                     /locus_tag="PC523_001047"
     CDS             20588..21454
                     /locus_tag="PC523_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474592.1"
                     /translation="MNKKDTLHLIFIIVIFILLWYVVVTVSEINKNIESCSNKLDYLS
                     QEIMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECIIDVDVQLSKLGENGKVFF
                     MYKEESNKWNETEMTKHGELSYVCEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFSSGIRDNREYFIEMVENSNLFNHESEKSKNKAYNNIRLEKVDIIVNEGKKD
                     TVYKAKLVESLDDGKTNNSNRNQVNYEASFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEN"
     gene            complement(21547..22038)
                     /locus_tag="PC523_001048"
     CDS             complement(21547..22038)
                     /locus_tag="PC523_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2474593.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKVLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKIGYTHMRLDTLPSMKSAQGLYKKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(22099..22539)
                     /locus_tag="PC523_001049"
     CDS             complement(22099..22539)
                     /locus_tag="PC523_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2474594.1"
                     /translation="MKLNVRIADINNWRDLVNLSVDKSQLDYIESNALSIAESKFITA
                     WIPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGRGYGKASLDFLVNHLKKEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            22700..23758
                     /locus_tag="PC523_001050"
     CDS             22700..23758
                     /locus_tag="PC523_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HDF2474595.1"
                     /translation="MNKINKNFKKIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNQDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNRYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            23900..24724
                     /gene="spo0A"
                     /locus_tag="PC523_001051"
     CDS             23900..24724
                     /gene="spo0A"
                     /locus_tag="PC523_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HDF2474596.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            25136..26257
                     /locus_tag="PC523_001052"
     CDS             25136..26257
                     /locus_tag="PC523_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474597.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSYQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            26275..27150
                     /locus_tag="PC523_001053"
     CDS             26275..27150
                     /locus_tag="PC523_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HDF2474598.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLANVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIVLKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNSLGNTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            27174..27866
                     /locus_tag="PC523_001054"
     CDS             27174..27866
                     /locus_tag="PC523_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2474599.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDINQINEIIVVDDGSIDN
                     TAKAVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADVIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            27874..28698
                     /locus_tag="PC523_001055"
     CDS             27874..28698
                     /locus_tag="PC523_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2474600.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            28703..29770
                     /locus_tag="PC523_001056"
     CDS             28703..29770
                     /locus_tag="PC523_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HDF2474601.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQRAYNYPNISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFVASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADVVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLNKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            29772..30308
                     /locus_tag="PC523_001057"
     CDS             29772..30308
                     /locus_tag="PC523_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2474602.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     VKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVHEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            30376..30981
                     /locus_tag="PC523_001058"
     CDS             30376..30981
                     /locus_tag="PC523_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HDF2474603.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPSSSNN
                     ILNNINPAVEYYNLNISIKDAVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILTLISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTVSIGIIKFLAK"
     gene            30993..31907
                     /locus_tag="PC523_001059"
     CDS             30993..31907
                     /locus_tag="PC523_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2474604.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLI
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKSQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDIELQKAEAK"
     gene            31949..33121
                     /locus_tag="PC523_001060"
     CDS             31949..33121
                     /locus_tag="PC523_001060"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDF2474605.1"
                     /translation="MSRVIWIVIDSVGIGALPDAENFGDSKDVSTLGNIFREYPDINI
                     PNMRKLGIGNIDGVDFFESIKTPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIDEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKKDLF"
     gene            33168..33980
                     /locus_tag="PC523_001061"
     CDS             33168..33980
                     /locus_tag="PC523_001061"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDF2474606.1"
                     /translation="MFEKIAQSSKFINAKINIKPKIGLILGSGLGDLANDIEDSVTIK
                     YSEIPNFPISTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEVLIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRLGDRFPDMSDAYSS
                     RYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQSLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            34183..35508
                     /locus_tag="PC523_001062"
     CDS             34183..35508
                     /locus_tag="PC523_001062"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDF2474607.1"
                     /translation="MRMYDIIRKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFINSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLIASSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFDLA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAYDFDDGVEKIRESIKSGDAIHKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYINKIKAEDVGVSAMILGAGRETKDDVLDLSVGIILEKKVGDYINEGE
                     VLAYMYYNDEQKLSLAKDRFIDSYSIVDSKVEKNKLIYGIVTKEGIKKF"
     gene            35721..36800
                     /gene="mltG"
                     /locus_tag="PC523_001063"
     CDS             35721..36800
                     /gene="mltG"
                     /locus_tag="PC523_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDF2474608.1"
                     /translation="MNFKENRLKIAALIIVILIILAGIFVSMQIGPYNKNNKKDVIID
                     IPNGASIGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKKLNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPDKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            36972..37646
                     /locus_tag="PC523_001064"
     CDS             36972..37646
                     /locus_tag="PC523_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDF2474609.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKIQKPKRVLEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFENNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLIPIGDGVALSYKESKRG
                     DVDGIK"
     gene            37633..38880
                     /locus_tag="PC523_001065"
     CDS             37633..38880
                     /locus_tag="PC523_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDF2474610.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YKDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEAMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            38952..40616
                     /locus_tag="PC523_001066"
     CDS             38952..40616
                     /locus_tag="PC523_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDF2474611.1"
                     /translation="MSKKKTPFLKKVGKRSWCVFTIILIIYSALIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKYQVLVVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQAKNIDKNMKKELEKNGIMIEEKTMRYSKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTID
                     NKDKDRHLKTTIDYNIQRKLEQILNKEENPTAAIISEVSTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIMANNGTFKPLYLYKSLVDNDMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRTGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIQ"
     gene            complement(40714..40998)
                     /locus_tag="PC523_001067"
     CDS             complement(40714..40998)
                     /locus_tag="PC523_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2474612.1"
                     /translation="MCNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPRER
                     EIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(41024..42538)
                     /locus_tag="PC523_001068"
     CDS             complement(41024..42538)
                     /locus_tag="PC523_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2474613.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQSLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADIVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKVYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHNYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKTSDIDLDKNNKDSNIEFQKRVLKGLETELK
                     ELSKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNAKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAVYYKSKEQKLDSFELMVHLKLHED"
     gene            42720..43532
                     /locus_tag="PC523_001069"
     CDS             42720..43532
                     /locus_tag="PC523_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474614.1"
                     /translation="MCNFKKILGLFMIIMLSVSMVACSSGGDSKDESKGKDKQFIEDL
                     GKGLKKRWDLASKEEAGTLNLEQNEAYEKFVEVEMDAVSKYKDAEFDSPELGKLAKDY
                     IKALEDSKEAIKYYSNDQLKFSELWEKPYNERSTILLTLVDKFGLKVDEKEFKVLKDN
                     AQIVNEENDLKEKVNAMAKGIKFEKVKDEYDWKTYQATVENTSDLDLQDFYLDINLID
                     KDGVIIETQTSTSVNGTWKKGQKVRLEFSTDKDFEKIEWTPHYSANNYSITE"
     gene            43572..45017
                     /locus_tag="PC523_001070"
     CDS             43572..45017
                     /locus_tag="PC523_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2474615.1"
                     /translation="MKIKKLLTIGLSLSMFIASCHISANALDKIESIKGADKYETTGI
                     IADKQNYTTAILINADSTMADGLSASGLAGATNAPILLTKKNNIPNATLKRLEKAKKV
                     YIIGGENSIDKYTETVLKGKGIEIKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAF
                     KGEPDAMSAAPVAVREKAAIVLTDGKSVPFNTTGVESYVIGGTSSMNDKLVNDTNSTR
                     LGGTDRYDTNKKIINKFYGGVKEFYIASGTDLVYALVGSTVAKNNAIVLVNNDSNKSV
                     LKSTTKLTAIGNLSDSILQQCLNVTKNIGDSNTGRQYTVEDAANAVKKLLGVKKFYYN
                     ENYPEDEDVIFLLDENFEFSGKQFYAFGYVTENSEGDVRYCVEKNDMSKVYTYNVSGV
                     MQVCNPDDYKKKEMISEEKAKQLALEECARKHKVGVDLLKVNIAYIDDFDDIKVYAVR
                     IDSKPHPNQTYGWYFIDVYSGYAYKDVWNNI"
     gene            45155..45313
                     /locus_tag="PC523_001071"
     CDS             45155..45313
                     /locus_tag="PC523_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474616.1"
                     /translation="MDNFLLSILASILASYIVYLISKLFRKRKKPLKAGTKSGWELDF
                     KIKFHKFK"
     repeat_region   45600..46160
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=45600..45628
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaag"
     gene            complement(46238..46372)
                     /locus_tag="PC523_001072"
     CDS             complement(46238..46372)
                     /locus_tag="PC523_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2474617.1"
                     /translation="MFEKTNINTSVMNIIESDERPIRDDELIIFAKIFGVSINYILGL
                     "
     gene            46551..46802
                     /locus_tag="PC523_001073"
     CDS             46551..46802
                     /locus_tag="PC523_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474618.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDKEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWEEFNEVAKEVAINIAMKKVETVES"
     gene            complement(46967..47155)
                     /locus_tag="PC523_001074"
     CDS             complement(46967..47155)
                     /locus_tag="PC523_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474619.1"
                     /translation="MIGFKKRDTKYLLKIVARAHGISVAEAIVEMQTTINNARNNPDP
                     EKQAEFIISLNTIFTKNL"
     gene            complement(47613..48272)
                     /locus_tag="PC523_001075"
     CDS             complement(47613..48272)
                     /locus_tag="PC523_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HDF2474620.1"
                     /translation="MNIVVGVLSILSAFCIAGSMIGLMFSFGYPKIIRENPIIILKRM
                     QNKKTMISLLFYLFGIGGFMVVQASIALRFIEQSKGEFIWSNFAYINGIIYGVLLFVG
                     ILRYTKTFPMLSNQMINKQITEKEAGQLYKVLNTYIGETMTEHVAFVFLSFMIFCNSM
                     SLLSINYVANWIGLLGIIIAIGLFIGNLEFLGIKKLFIVNRVFSSLSAFWLLLLGICI
                     I"
     gene            complement(48328..48765)
                     /locus_tag="PC523_001076"
     CDS             complement(48328..48765)
                     /locus_tag="PC523_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2474621.1"
                     /translation="MEEHEIFNTFVQILNWASERNKNSKSYGTDDKLYIAEIHTLVMI
                     GKMPGILQKELCKEVGVTKGRMSVIIAHLQEKNLIEMRREQGNGCTIPIYLTTLGEQI
                     YHNHNEQEMNMKIQIYQVLSKCSQEEIEKFNGILRDILEILRQ"
     gene            complement(49089..49253)
                     /locus_tag="PC523_001077"
     CDS             complement(49089..49253)
                     /locus_tag="PC523_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2474622.1"
                     /translation="MFFHSTYNNGSYISHIGIYIGNNHMYHVNNPIGYADLTSAYLQQ
                     HLIGAGRIKQ"
     gene            49789..50298
                     /gene="vanZ1"
                     /locus_tag="PC523_001078"
     CDS             49789..50298
                     /gene="vanZ1"
                     /locus_tag="PC523_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HDF2474623.1"
                     /translation="MKSKKYNITKGLFIVYIIILTWIILFKLQFDVSSIETMNLRSIN
                     LVPFAGSLIINNKVDISEIILNVAIFIPFGIYICMLKEEWSFIKKVIPIFITSLAFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINILAFIVTIIVVLF
                     LGLVIFANL"
     gene            complement(50407..50601)
                     /locus_tag="PC523_001079"
     CDS             complement(50407..50601)
                     /locus_tag="PC523_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HDF2474624.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIQEFQDTIIL"
     gene            complement(51094..51510)
                     /locus_tag="PC523_001080"
     CDS             complement(51094..51510)
                     /locus_tag="PC523_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2474625.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEIHTTFYMLFSTIIYI"
     gene            52077..52439
                     /locus_tag="PC523_001081"
     CDS             52077..52439
                     /locus_tag="PC523_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474626.1"
                     /translation="MKIRKRGFISIECVISIAILYVAVYLVSTSLYSCYSFVSRNISD
                     RKMLSTAKKYIEDEKYRIQNSKYELIENKIEKNYINGYEISSRVEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            52423..53142
                     /locus_tag="PC523_001082"
     CDS             52423..53142
                     /locus_tag="PC523_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474627.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFKLGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDISNSEGEFREYFLGNNMIECIKKIKDTSYSS
                     VEGLSLKVENNTIYDREDFFEFDIESSVREKEFLKTIKVRVKIKNPWVGIDIEDYMNK
                     SNNNKIYDKKFNNYKASENNEILNQFKNESENISNQEVVEPTEEDNEEYEEELKQNII
                     ENIKKNFDEKKLVQVYKYRGV"
     gene            53162..53581
                     /locus_tag="PC523_001083"
     CDS             53162..53581
                     /locus_tag="PC523_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474628.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNIENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIILPKNVKIARSIETIKFKSDG
                     SPQKASTIEIYNNRLRKIITITPVSGRVLLKEGKYET"
     gene            53571..54101
                     /locus_tag="PC523_001084"
     CDS             53571..54101
                     /locus_tag="PC523_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474629.1"
                     /translation="MKHKYGYLLLESVVSLSSMLIMILVLYSIFLSTISLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIINVSNYEDTFKKATSIKCRYVDENVNNNEESISNK
                     EIILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSINNNGQYVNIKLKLSKRSQK
                     YETEFKIKVWNFSENI"
BASE COUNT        21558 a         5670 c         9505 g        17438 t
ORIGIN      
        1 ataggattag ataggtaata ggttaagtta aaataaccca ttacctattt ttatttgtag
       61 gaaaagtgtc tatgaaatta aaagtaaaaa ataccactaa taaaacgatt gaaaacacta
      121 gataaataat aaataaaaat aattataaat tttggcacat atattgctaa tcatagaaat
      181 aagatgacaa aataataggg aggtttaata atgaaataca aaacaacagg gacttgtgct
      241 acagaaattg aatttgaggt aaaagaaaat aaagttacaa atgtaaactt tataggtgga
      301 tgtgatggaa atttaaaagg gataaaagta ttagttgaag gcatgaatat agaagatgta
      361 atacaaaaat taaaaggaat agagtgtaaa acaaagccaa cgtcttgtcc agaccaactg
      421 tctctagcat tggaaaatta tatgaatata aaataataca taaaacaatc gattagttag
      481 gagtttaatt atgaaaccaa taatagggat tttaggaaat ttaataataa tggaaaatgg
      541 tatgtttcca ggtcttgaaa ggtcatatgt aaataatgat tatataaatg cagtactaaa
      601 aggtggaggt agtccagtaa tcattcctgt aaatacagat aaagaagtta taaaaaaaca
      661 gattgaaatg gttgatggag tcttaatatc tggaggatgg gatgttaatc ctcaactgta
      721 tggagaagaa acaagagaag aaacaacatt tatatatcca gaagtggatg aatttgattt
      781 aattgcaata aatatagcat tagggcttca aaaacctata ttaggtatat gtaggggact
      841 acaaatttta aatgtaagtt taggaggaac tttatatcaa gataataatc ttaaagaagg
      901 aagttatata aaacacacac aatcttccaa aagacatgta gctactcata aagttgatgt
      961 taaagaagga agtattttag aaggaatatt gggaaaacaa ttacttacaa atagttatca
     1021 tcatcaaagt gtcaaccagc ttggaaaagg attaaaagcc atagcatact caaaagatgg
     1081 tataatagaa gctattgaga aagaggatga aaaatttgta gtaggagtac agtggcatcc
     1141 agaaatgatg gtagactact gtaataaaat ggaaaaacta tttaaatatt ttataaatat
     1201 ttgttcaaat ttataatata ccttggaggg gattgaacat gaacgaaaag aataaaatgg
     1261 gattgataag tattatactt ttaggcatta atgcagtagt aggagctgga gtttttctat
     1321 taccaggaga tgcaatgaag gcatttggtg tagcaagtat atttgtatat atgtttgata
     1381 tgttattagt tttatctatg gcattctgtt ttgctgaggt tgcaggtaaa ttcaataaaa
     1441 atggtgctgc ctatgtatat accaaagaag cttttggaga tttttgtggt tttgaagtag
     1501 gtcttatgaa atgggtaata ggatgtatct cttggggagc tttaatagta ggttttccta
     1561 catccttatc agcagtatgg gctccagcag gtgaaccaca tatacaaaaa attataattg
     1621 tagccatgat agttggactt acaattataa atttattagg tgtatctcta tcaaaaattg
     1681 tacaaaatgt tataactgta ggtaagctaa ttccattaat attatttata ggaataggta
     1741 tattttttat aaaaggagtt aattttacta cttcaacaat ggtgcctcca ggagctggag
     1801 ctacagagtt tggagcagca gcacttttaa tgttttattc atttacagga tttgaatcta
     1861 tagcagttgc agcagaagat atggaaaatc cacaaaaaaa tataccaata gcaattataa
     1921 gtgtaatagt aattgcatca ataatatata tattaaatca agttgtatgt gttggtatac
     1981 taggagattc attatctagt acatctacac cagtggcaga cgctgcaaga gtatgctttg
     2041 gaaatatggg agctggactg gttacatttg gaacattggt ttctgttggt ggaatttgta
     2101 tgtgtggcgc ttttgtaaat ccaagaagtt gtgtagcttt agctgatgat aaaatgttac
     2161 cgagaatatt tgcaagaaaa gataaaaaag gtacaccata tgtagcaata atagctacta
     2221 tgttaattac tatcccaata gctctatctg gaagttttgc tgaattagca gcaataagtg
     2281 cagttgctag atttatacaa tatataccaa catctttatc agtattagtg tttagaaaga
     2341 aaagaccaga attagtaggt acttttaaaa ctccttttgg agcagtgatt cctctaattg
     2401 ctgtatgtgt tggaacatgg ttattattcc aagcatctat gcatcaatta attatgggat
     2461 taggagcttt ggcaattgga gtaccattat actttataat gaagtcttat aatagaaaag
     2521 tttatggtga aaatttaaag aaaatagttt aattatgtta taacagttta ttgatttaaa
     2581 gttagatatt ggcagaaaat taaatacaaa taagtatcta taaggctata ttaaaatatt
     2641 attgattttt tattttaata tagccttata ttgttaatca gtaagtgaaa aatattgtaa
     2701 caacaaaaga acaatagtat tatgtttata gacaaaaact tattaggagg taaagttatg
     2761 ccaacaataa cattaaaaaa tggtgtagat gtacttatta gagaaggagt aagagaagat
     2821 gctcaaaaca ttatagattt ttataatgaa gttggtggag aaactcattt tctgtcattt
     2881 ggaaaggatg aatataaaat atctttgaaa gaacaagaaa atgctataga atcagcaaaa
     2941 gcatctgata attcggttaa actgattgca tttatagatg gtgaaatagt tggtattgct
     3001 acaattgatt ctaatcaaaa ggcaaaagga aagcatgttg gagtattagg aattgttgta
     3061 aaggaaaaat attggggaat tggactgggt aaaaggctta tgttagacct tatagaatgg
     3121 tgtaaatcaa atggaataac caagaagata actcttgtca ctaatgaaga aaattataat
     3181 gctataggat tatacaaaaa agttggattt gaagtagaaa gcatactaaa gaaagaatgt
     3241 tattataatg gtgtttacac tgatttaata ggaatgtcac ttttgttggg aatataatta
     3301 gtgtacattt tatatcgaaa aaactatatt aagtatggta taattaaaaa agaatataac
     3361 ttaatataat tttggaggac gaattatgaa aaatctgtgt aaaatatcag atgattattt
     3421 aaacagcaag cttaagtatt taaagttatt atcaaaacaa tatccaagta tatcaaaagc
     3481 aagtacagaa ataataaatt tagaggctat attaaatctt ccaaagggaa cagagcattt
     3541 tataacagat gtacatggtg aatatgaacc atttgtacat gtattaaaaa atggttctgg
     3601 tgtaataaag agaaaaatag aagaattgtt ttcaaataca ataagagaca gcgaaaaaaa
     3661 aatgttagca actcttgtat attatcctga acaaaagtta gacctaataa ttaagcaaga
     3721 ggaaaatata gatgattttt atagaataaa tatttatagg cttatagaac tttgtaaata
     3781 tgcttctagc aaatatacta ggtctaaagt cagaaaactg ttaccagaaa attttaaata
     3841 cataatagaa gaacttttgc atgagcatgt taaaagtgaa cataaagaag agtactataa
     3901 aagtatagtt gaaacaatag ttgatatagg aatagctaaa gagtttataa ttgctatttc
     3961 tacagttata caaaaattag ttgtagatag acttcatgta ataggagata tttatgatag
     4021 aggtccaaga ccagatataa ttgtcgataa gcttattgaa catcattgtg tggacattca
     4081 atggggaaat catgatatct tatggatggg tgcagcatca ggtgaaaaaa cttgtatagc
     4141 aaatgcactt aggatatcag ctagatatgc aaatttagac attgtagaag acatatatgg
     4201 aataaatcta ttgcctttag ccacttttgc gatagagatg tataaagatg acccttgtaa
     4261 agaatttatt ccaaagatta atgaccaaag tgtaacaaca acagaaaagt ctttaatggc
     4321 aaaaatgcac aaagcaataa gtataattca atttaagctt gaaggtgaag ttataagacg
     4381 aagacctgag tttgagatgg aacatagact tttacttaat atgataaact atgatgaagg
     4441 tacgattact ttaaaaggaa aaacatataa attaaaagat acttatttgc ctacaataga
     4501 caaaaaagac ccatataaat taactataga agaaaaaaat gttattgata aattagtatc
     4561 ttcatttaga ggaagtgaaa aattacaaaa acatgtttca tttctgttct ctaaaggaag
     4621 tatatattta aaagctaact caaatttact gattcatgga tgcgtaccat taaatgaaga
     4681 tggaagcttt atgtctatga atataatggg caaagagtat aaaggtaaag cccttatgga
     4741 tagaatggag tctctagcaa gagaaggatt tttctttaaa gataaggcag aagaaaaact
     4801 gtatggcatg gatattatgt ggtatttatg gactgggaaa tgttcatcat tatttggtaa
     4861 agatgatatg actacatttg aaagatactt tatagcggaa aaagagactc ataaagaaaa
     4921 taagaatcca tattttaaac ttagagaaaa tgagatggct tgtaaaaaat tatttgaaga
     4981 atttgattta gaacttgatg aatctcatat aataaatggt catgttcctg tagagagtaa
     5041 aaatggagag agcccaataa aagctaatgg taaaattctc gttatagatg gtggtttttc
     5101 tagagcttac caaaaaacta ctggtatagc tggatacact ttgatataca attctagaac
     5161 tttgcaatta gtatcgcatg aaccatttaa ttctgctgaa gaagccattg caaatgaaag
     5221 tgatatctta tcaacaactg ttgtagtgga acataaggca aagagaaaaa tggtgagaga
     5281 tactgatgaa ggagtaaaaa tacaagagga aatagaagat ttaaaactac ttttaatggc
     5341 ttataagaaa gggctaataa aagaaatgta gatactatag aggcacttca taattgaagt
     5401 gtctttttgt taatacttat taaatatatt atatagatta ataatagtaa tttataacct
     5461 tatacttact aagtgaatcc aagataaatg ggttcacttt ttttatttat aaacattata
     5521 aaaaaatata gatgttttgg ttatattcct tgtcctttat aaatagaatt atataaacaa
     5581 aggaggagga caagaaatga ataaactttc tgatgaaata ataaactctc tttctacaac
     5641 tataagagaa aaggtagaaa aaatctcaaa caataatcta aacatagaag aaattaggtt
     5701 acgctcacaa aaaccattga tactaaatgc aaactctaaa gattattttt acaatcaaaa
     5761 aactatggca ctcgatttaa atcaacaaaa ctcatatgta gtaacaagag aagatgtaga
     5821 gcaaactttt caaataatat gtaaatattc aatacattca tttatggatg acataaaaaa
     5881 aggatttata actttgagag gaggtcatag agtaggatta gttgggaaag taatagtaga
     5941 agatggacaa gtcaaaaata taaagcatat atcatcttta aatataaggg tatcaagaga
     6001 aattattgga tgctctgata aaattttaag tcatatcata aagggaaaaa atcagattaa
     6061 taatactttg ataatatcac cacctcaatg tggaaaaaca actttaataa gagatatagt
     6121 aagaaattta agtaatggta atgaagatta tggatttaaa ggtttaaaag tagctttagt
     6181 agatgagcgt aatgagatag caggagctta tctaggtgtt ccacaaatgg atgttggaat
     6241 aaggactgat ataatagaaa cttgcccaaa agacttgggt attacgatgc ttttaaggtc
     6301 tatgtcacca aatgttattg tgacagatga aattggaagt gaaaaagaaa taaaggcatt
     6361 atacacagca ttaaatggag gtataggatt aataactact gtgcatggag attcaataga
     6421 tgatatacaa aatagaaaag aattaaatag acttctggat aaagagttat ttaaaaaagt
     6481 tataattctt tccgcaaaaa gaggagcagg aacgattgaa aaaatttatg atttagaaga
     6541 aaagagatgg tattttgcaa attaaaataa ttattatagc tctcttgata ggaagtagtt
     6601 atttgatagg tgaacaaata tacaaaactt atactcgtag acataaacaa ttgaacgatt
     6661 taataagagt tttagaaatt ttgaggatgg atttatcttt cggactttat acattggaag
     6721 aaatctttaa tagaataggt agaaataaag agttttgttt ttggaaattt ttctatcaaa
     6781 tatcagaagg acttcacaat gaacaaagta aaactttgga aataataata tcagagaata
     6841 ttgatgtctt aagcaaagaa acttatctag gcaataaaga aattgaggaa ctgaaaaatc
     6901 taatacttac tttagggaaa agcgatatag agtcacaaca aagaatgata gatttatcta
     6961 tagaaaattt gaaaaaacaa acctatgaga caaaggaaga tataaacaaa aaaggcgtat
     7021 tgtataaaaa attagtgaca tttataggta taggtatatg tattatattg atttaatatg
     7081 gggaggttca ttatggaaat atcattgata ttaaaagttg caggtgtcgg tattctaata
     7141 tcagtattga atatgatatt ggaaaaaaca gatagaaaag attgggcagg tcttactaca
     7201 ttagcaggtg taataatcgt attgggtatg gtaataacag aaataagtga tttgtttaat
     7261 actgtaagaa caatgtttca actttactaa agaggaggga gtaatttgga aataatgcaa
     7321 ttaataggaa ttgcaatcat ttcaactaca ctctgtttag taattaaaaa agatagacca
     7381 gagatagcaa atttcatagc aataataact ggagtaataa tactattgtc agttatgttt
     7441 aagctaaatt ttatagtaga tagtgtacaa gatttggcta acaaggctaa catcccaact
     7501 atgtatatat cattaataat aaaattaatt gggatagcat acctaatgga atttgcgata
     7561 caactttgta aagattgtgg ggaaggaaac attgcttcaa aactagaatt tggaggtaaa
     7621 ataatagtta tgtcaatgtc tttccctata cttttgtcca ttgtagaaat ggtggtgaat
     7681 ataattccat agatgtgtat cttggaatga ctataagtat attatagagc tttcgttgta
     7741 ttgaggttta tagagtgaag agaggcatgg ttgcttatga aaaaaagatt tttaccaatg
     7801 attataggtt ttttatttac tttctttttt gtaaatatct ttgctattat gatatttgct
     7861 aatgaagtgc caagtagtga aaataaagaa tatggtgaaa caaaaaatag catagataaa
     7921 tatatagatg gtcaattaga caagctggat ataaatgaaa ttcaagacta tataaataaa
     7981 gaaatagtta ttaatgatgt taatttaaaa tcatttgtaa aagatttaat tagtggagaa
     8041 aaaaatatct tagatttatt caataaagat ggtttgaaga tattgatgtt tgatgaattt
     8101 aaagcaagtt taaaggtagt agcagtgatt ttggtattgg ctctgttatc atccatttta
     8161 aagagtctgg aaaattcttt ttcatcaggt gcagtaagtc aaatagcaac atatataata
     8221 tttatcacaa tggtatcact tacacttgtt ggttttaaag atgtattgca gatttgctat
     8281 gatgcaatag accatactgt aggtcttatg caagttataa tgccaatttt aataacattt
     8341 ttacttttaa ttggattccc aataacgtcg actactttga atcctatttt cataggagga
     8401 gtaacattca taaatgtatt ttttaaaaat tttctatttg tatctattac tgttgcattt
     8461 ggaattttaa taataaataa tttatcaaaa aatataaggc taaagagatt tttctccttt
     8521 gtaaaacaaa taaattatgt ttctattgga gcaatgttta ctatttactt aggtcttgta
     8581 tctatacaag gattgtatgt aaccagtttt gataaattca gtgtaaaaac tgctaaattt
     8641 gctataggta attttattcc tgttgtcggg ggatttgtat ccgattctgt agatatttta
     8701 ttatcttcat ctcagcttat aaaaaatata tttggaggaa tagggcttat attacttgtt
     8761 ggaatttgtt tacttccagt gataaaaatt ttatcagtaa tagtagtata caaattagca
     8821 gcaataattg tagaacctgt tggagaggat ggaatatcta attttttaaa tgaagtggca
     8881 aatttaatga taataatgct agcatcagta attgctataa cagtaatgtt ctttgtaact
     8941 gtagctatct tgacctcgat aagtgtcgta agtcaaggat aatttatata tcaattaaaa
     9001 ataattcatt taatctaaag tttttagaga ggaggatgct atgttagagg gcataaaggc
     9061 gtggatagtt agtgttttga taggagcatt tatagtaaat atagtagata tgattttacc
     9121 aagttctaag ataaaacctt atgtaaacct agttttaaat tttatgtttg tatttattgt
     9181 aataactcca gtggtaggat ttttttcaaa agatatgagt ttagaagata gaatattgaa
     9241 atctatggga agctacaaca aacagtatgt agatagtaca aatgctttag caaaagagac
     9301 tggaaataat agtctatcta agggatatga ggatgggtta aaagaagtac ttaaattaaa
     9361 acttgatgaa tatggttatg atttagaaga tatagagctt aatggagcaa atataaataa
     9421 tataaaaata aaagaaaaga acaataatgc aaaaagtaat agcagtgcca atgaagaaaa
     9481 taaaaataat agtactaaaa gagaggaaga aaacataaac tctaatgaca aagaaaattc
     9541 taaacaagtc ttcaaaaaag gtacagagta tgggttgagc ctaaatgaag aaaaattaaa
     9601 aaatgactta ataaaggtgc tggatgtttc tatagaagat atacaaattg ataaatagga
     9661 gttgggagaa tgttgaaaaa cttaaatgac aaagataaga gaaaggtata ttcactaata
     9721 actatagcag gaatatgtgt ggtttcatta gtattattat cttgttttcc tagtagcaaa
     9781 aatgataaac aagttggaaa aacagagacc aataaaaatg ctgaaaagca agtcactaaa
     9841 gaacaagaaa aagatgattt agaatcaaaa ttaacagcaa tactatctaa aattgatgga
     9901 gcaggagatg ttgatgtaat ggttacattt gaatcaagtg aagaaataca acctgctttt
     9961 aattcaaata atacaacaga aacaacagag gaaaaagatg cacaaggtgg agaaagaaca
    10021 gttacgactt ctagtgaaaa taaaacaatg ataacttcaa attcaagtga ccctgtagtt
    10081 ataaaaacta cagagccaaa gataaaggga gtgatagtgg tagcaagtgg agcaagtgac
    10141 ccaagcgtta aagagacact ttatagtgca gtacaaacat cattacaagt agctggtcat
    10201 caagtagaaa tatattctaa ataaatacat ataaccgggg ggataaatga atgaagttta
    10261 attataaggg aagaggattt gtaataataa ctttaactgc aatgttagtg gtagtaggaa
    10321 cagtcaatta tcaattaagt aagaaatctt tattagaaac atcaaaagag tttaaggcat
    10381 atgaagaagc gcagttacaa aaaaatactg atgatagtga tagtagtaag acagaggatt
    10441 ctaataatgt agataaacaa gatggtaagg aaagtgctga tattgatata gttgatagta
    10501 aaactagtaa agtaaaagaa aaggcaactg agacaagtaa ggaaataaaa gctcaattat
    10561 catcagagaa aaatatgaaa aaggcatctt atattttaga tatgaaaatg aatagagaaa
    10621 aacaaagaaa tgagttggtt caagatttaa atgaaatgat aaataatcca tctacaacag
    10681 aagaatctag aaaagaagct tctaatatga aattaaatat agttaaaata caagaaaaag
    10741 agcttcaaat agaaaatctt ttgagtacaa aaggatatga agaagcatta gtttatataa
    10801 gtgataacaa agttaatgta gttgtaaacg aagctaaatt agaaaagaaa gatgcagcaa
    10861 aaatatttga tttagttgca gaacaagcca atgtaaagta tgaaaatatt aaacttacaa
    10921 ataataataa taagtaaagt ataaacctca gaattaactg aggttatttt cttgcaaagt
    10981 agacttgtat ttggtattat tatacttata aaaatagttc cttaaaatca ggaggtaagt
    11041 tttaatatgg aagataataa atttggacaa gtaaaaatat ctaatgatgt aatagctaca
    11101 atagctggtt tagcagcatt agaagttgaa ggcatagaaa ctacagcaac attaacagat
    11161 aagttgttaa aaaataatgg agtaaaaata caaatcgaag aagaggatgt taatctagat
    11221 gtaatggtta cgataaagta tggaatgtca ataccagata cagcttttaa agttcaagaa
    11281 aatgtaaaaa atactgttga gacaatgaca ggactgaagg tttctcaagt aaatatacac
    11341 attcaaggaa tcagctttaa gaaggataaa gttgataaag aagaagcaaa agcaactaag
    11401 aaaaattaat attagccctc tgaaaagtca gagggttttt tgctgataat tgattttata
    11461 tggataatta aacttaaagg agaattttaa ttgatgaaaa aagatagggc acaaaagagt
    11521 acaacaagag aatatataat gaaacttata taccaaataa atattaataa agaagatttt
    11581 gaaactttag aagatagggt agataatttt ttaaaagata attcagaaca tattataaat
    11641 agatataagg agcttgcact tcagtattca aaaaatacaa atttaaagtt agaagatact
    11701 gaaattgaag atgttataga caaaaaatat ataaatacag tatgcaaagc actaaaagaa
    11761 aatcatgaca agatagatga gttaataaat aagcatgcta aaaactggac agtagataga
    11821 atgccaaaag tagatgtttc tatacttaga ttatctgtgt gtgaaatact ttatttagac
    11881 actcctaata aagtatcaat aaatgaagca gtagaacttg caaaaatata ttgtgatgat
    11941 aaatctccta aatttataaa tggtatattg ggaagtgttg ttgatgaaat tgggaaataa
    12001 tataataatt ggaattgata ctagctgcta tactacttct atagcagcta tctctttaga
    12061 taagaaagtt atattcaatg aaaaaataat gttagaagta atggataatt caaaaggttt
    12121 aaggcaaagt gaagctgtat tccaacatgt caataattta gggattctta gtgataggat
    12181 aaaatctttt aaagataggt ttcatgtaga aggggtttgc tcatcaaaaa aacctagacc
    12241 tgttgaaaat tcatatatgc ctgtattcaa tgtaggtcat aattttggga gattattgtc
    12301 tagtatatat ggttgtagat tttatgagac tactcatcaa gagaatcaca ttgaagcaag
    12361 tttattaaat agtaaattaa aaaataataa tagatttatt tctgttcata tgtctggtgg
    12421 tactacagag atacttttaa caagcaaaca agatagtcat cacaatgtat gcgatattga
    12481 tttaagtaaa tttgccaaaa caagtattaa aaaagatgat aaaagtaagt tatacaataa
    12541 ttttggatat aatatagata taattggagg aagtaaagat attagctttg gtcaacttat
    12601 agatagggta ggagttaagc ttggatataa atttccttca ggaaagtatt tagatgaaaa
    12661 tgctttaaat tgtaatctta aaatagaaag tggtctgaaa acctctgtaa gagatggata
    12721 tatgaattta tctgggttag aaaatcaggt aaataagatt ataaaggata atggagataa
    12781 tgctaatcaa aaagaatata tatctaaatt agtattagat tcagtagtta gaaatatgtt
    12841 taaatcttta gtatatttat gtgaaactta taatgtaagt gaagtagtat ttgctggtgg
    12901 agtttctgca agtaagtata tttttagaga attaaccatg aaattaggaa aaaagcatat
    12961 agaagcttat tttacagaac cacaatattc aactgataat gctgttgggt gtgctataat
    13021 aggattgaat aactttttag gagaaagagt atgaaactaa gagctttaga tataagtgaa
    13081 gcaaattcat atattaaaag aattttaata aatgacccaa tactttctaa tcttaaagtt
    13141 aaaggagaga tttctaattt taaggttcat agtagtggaa atgtatattt atcacttaaa
    13201 gatgaaacat caaagttaaa ctgtgtaatt tttaaaagta attttaatcg taatctaaaa
    13261 ctagataatg gtattaaagt tatagctaat ggatatatat cagtttatga aagagatggt
    13321 gcgtatcaat tatacattaa tgaaattgaa atagagggaa tagggaattt acacatagaa
    13381 tttaatagat taaaggaaaa acttaataaa gaaggattat ttgaccctaa atacaagata
    13441 cctataccta aaatgccaaa ctcaattggc gttataacat ctccaacagg tgctgttata
    13501 agagatataa taaatgtgat aagaagaaga tatccgaaag ttaatataaa gctttatcca
    13561 gtaacagttc aaggagataa atcagctgaa gaaatctgtg aagcaattag atttttcaat
    13621 catatgaaga atgttgatac acttatagtt ggtagaggtg ggggttcaat agaagaactt
    13681 tggtctttta atgatgaaat ggtagcaaga gaggttttta attctcaaat tccgataata
    13741 tcagcagttg ggcatgaaac tgattttact atttgtgatt ttgtatctga tatgagagca
    13801 ccaacaccgt cagcagctgc tgagattgct actccatcat tggatgatat aaactataag
    13861 cttggaaaca taaaaagtag gttgagtaag tcccttacaa atcaaataga gctagaccaa
    13921 tatagattag aagcagtatt taataagata aataactatt tagattctta cacaataaaa
    13981 gataaagtta tacaattgga caaaatatat gataaaataa tttttggaat agaaaataat
    14041 ttaagattag aagatgaaaa gttagtaaaa attggagcat tacttcataa tttaagtcca
    14101 ttagctacta tggatagagg ttatagtata gctcaaaaaa atggaaaagt aataaatagt
    14161 ataaaaggat taaaaataaa ggatagtata gatatagtat taaaagatgg aaatttagaa
    14221 tgtatgatag aaaaaattga aaataaagag gtatagtatg aatttaacct acgaagaagc
    14281 ttataaaaga ctagagagca ttttaaatga gttagaatct aaaaatgcta gcttggatga
    14341 atctttaagc ctatatgaag aaggtataag cctttataaa cattgtaata agcttttaga
    14401 tgatgctaaa ttgaaaataa gtaaatttaa tcaattagga atagaagaag actttaaaat
    14461 agaggaggaa taaaattgga atttaaacaa tgcctaaaag aaaaagcaag ttttgtagaa
    14521 aaagtactta aagaatatat gcctaaagaa gaagggtatc aaaaaactgt tattgaagct
    14581 atgaactata gtttaagtgc tggaggtaag agattaagac caatacttac attagaggca
    14641 tgtaaaatag ttggaggaaa tgaagatgaa gctattcctt ttgctattgc aattgaaatg
    14701 atacatacat actcactaat acatgatgat ttgccagctc ttgacaatga tgatttaaga
    14761 agaggaagac caacaaatca taaagtatat ggagaagcta tggggatttt agcaggagat
    14821 gccttgttaa actatgcttt tgaagtaatg cttgcaggct caatcaataa agaaaatcca
    14881 gaaaagtatc taaaagctat aaatgaaatt gccaaaggtg ctggtatata tggaatgata
    14941 ggtggtcaag tagtagatgt agaaagcgaa aacaaacaga ttgaaaaaga aaaattagat
    15001 tatatacata tgaataaaac tgctgcaatg atggtaggct gtatgagagc aggagctact
    15061 ataggtggag caaattctga acaaatggaa gaaataacta aatatgcaaa aaatatcgga
    15121 ttatcattcc agatagtaga tgatatacta gacatagtag gagatgaatc taagttaggt
    15181 aaaaaggttg gaagtgatat agaaaatcat aagtcaacat acccatctct tttaggttta
    15241 gataaatcta aagaaattgc acataatttg attgatgaag caaagaaaag tatagaaaaa
    15301 ttatctgatg atgtagattt tttaaatggg ttggctgaat atataataga tagagaatac
    15361 taaaatatac agctaaaatc gaggagggaa aaatggattt tttttcggag atttttaaca
    15421 atggggcttt gggaataagt ctgattgctt gtttcttagc acaatttata aaaatattta
    15481 ctggtaagga aaaaagaatt gagttatcaa gaatacttat ttcaggaggc atgcctagtt
    15541 cccatagctc ttttgttaca agtttagcta cagtggtagg tattgaaaaa gggtttaatt
    15601 ctactgattt tgctataatt accgtgcttg ccttaattat aatgtatgat gcagcaggtg
    15661 ttagacgggc tgttggtaaa caagctacta tattaaatca aatggtggca gatatacagc
    15721 atgggaagca tatagagcag aagaaattaa aagaattaat aggacataca ccactagaag
    15781 tttggtttgg agctttactt ggagtagtaa cagctttaat attgatgtaa attgaaaatt
    15841 tacagaataa aggtgaggat aatatgtata aatatttaga taaagtaaat tctccaaagg
    15901 atataaaaaa tatgagtata gaagaaatgg atttacttgc aaaggatata agaaagttct
    15961 tagtaaaatc tgtttcaaag acaggaggac acttagcttc taatctagga gtggttgagt
    16021 taacattagc acttcataaa gtatttgaca gccctaaaga taagattgta tgggatgtag
    16081 gacatcaatc ttatgttcat aaaatagtta ctggaagaaa agattgtttt gtatctttaa
    16141 gacagtttaa tggactaagt ggttttccaa aggagagtga aagtcctcat gatatttttg
    16201 acacagggca tagcagtaca tctatttcta tagctacagg tattgcctgt gctagggata
    16261 taaaaaaaga aaactatagt gttatatctg tgataggaga tggttctata acagggggta
    16321 tggcgcttga agctttaaat caattgggat atataaatac caatatgata gtaatcctta
    16381 atgataatga gatgtccata gataaaaatg ttggaggtat gtctaaatat ctgtcaagta
    16441 ttataaggaa ctcaacagtt gagaaaatga cagatgaagt tgataaaatt ttaaatgtta
    16501 cacaaacagg tgaaatttta tctaagactg cacatagatt taaagataaa ctaatgtata
    16561 gcttttctcc tcaggactgt tcattttttg attcattagg tataagatat tatggaccta
    16621 tagatggtca taatactaaa gacttaatag atatattgag aaaagctaaa cacaaaaaag
    16681 gacctgttct tttgcatgta attacgaaaa aaggaaaagg gtacaggttt gcagaagaac
    16741 aacctgataa atatcatgga gtatcaaagt ttgatataaa gacaggtgtt acatctggga
    16801 aagtcaagtc tatgtcaact agtgttggag aaaaacttgt tgatatggct aataataatg
    16861 aaaatatagt agcaataaca gctgcaatgc catctggtac agggctaaac ctatttgaaa
    16921 gtgcatatcc aaaaagatat tatgatgttg gtatagctga acaacatgca acaggatttg
    16981 cagcaggtct tgcaaaaaat ggtatgaaac cttattttgc tgtgtactca tcatttttgc
    17041 aaagggcata tgaccaagtt attcacgatg tatgcataac aaaaaagcca gttacttttc
    17101 ttatagatag agctggatta gttggaaatg atggagaaac tcatcatggt atgtttgatt
    17161 taagttactt aaattctatt ccaaatattg tagtaatggc gccaaaagac acaagagagt
    17221 tggaacttat gatggatttg tcattaaaat tagattgtcc actagcaatt agatatccaa
    17281 gaggaagtag ttattaccta gataagggag aatatggaga gattgtattg ggtaagtatg
    17341 aggtattaga taatgggcaa gatacagtta tactttgtat tggaagtatg gtaaaacatg
    17401 ccttagaggc taaagaaata ttatcaagag aaggaataaa tccgacaata gttaatgcta
    17461 gattcttaaa gccaattgat gaagatatgt taaaagtatt attaaaaaat cataaaaatg
    17521 tagttactat agaggataat attgtaactg gaggatttgg aagtagaata aataagttta
    17581 ttatagataa tgaatataat gtaaatatac ttaatatagc aataccagaa gaatttgtaa
    17641 aacatggcaa tgcagacgaa ttatatgatt ttgtgggatt atcgccaaag agtattgctg
    17701 ataaaataag gaaattattt attaagtaaa aatttaatgt agatattgta aattaaaact
    17761 tatataaaat ataaaattta tcaattagaa taaatatcaa gtgtgttact tcaagctact
    17821 ttagttatta ttaaagtagc ttttaacaga aaggtaattg tatgaaaaaa agaatagatt
    17881 tattgttagt agaacaaggg cactttgaaa gtagagaaag agccaaaaaa gcaataatgg
    17941 ctggattggt ttttgttgat aatcaaaggt gtgataaggc tggtacagaa gtaaaagaag
    18001 actgtaatat agaagtaaaa ggaaatccaa taccatatgt tagtagaggc ggtttaaaac
    18061 ttgaaaaagc aatgaaaaat tttgatttga ctattgatgg gaaagtatgt atggatatag
    18121 gtgcatccac aggtggcttt acagattgta tgcttaaaaa tggagcaata aaagtatttt
    18181 ctatagatgt tggttatggt caacttgcat ggaagttgag acaagatgat agggttgtgt
    18241 gtatggaaag aaccaatata agaaatgtga caatagaaga tacaaagcag tttgcagatt
    18301 ttgcatcaat tgatgtatct tttatatcgt taaagttagt tcttccaaag gcaaaggaat
    18361 tagtacgtca tgatggggaa attgtcgctt taattaaacc tcaatttgaa gcaggaagag
    18421 aaaaagtagg taaaaaagga gtagttagag agaaatctac acatatagaa gttataaaaa
    18481 tgatttctga tttttcagtc gaaaatggat ttgagatttt agggttagat ttttctccta
    18541 ttaaaggtcc tgaaggcaat atagaatatt taattcattt gagaaatgga aatgatggat
    18601 atgaatttga tggtgaaata tacaataaca aaatagtaga agttgttgaa gcttctcata
    18661 atttagaaaa ataggttaaa agggggctta gatttgatcc tcgaattata tatgaaaaat
    18721 tgcgccttag ttgaagaact aaggttgaat attgataaaa acttaaatat acttacaggt
    18781 gaaactggtt caggaaaatc tataataata gatgcacttg gactttgttt aggtgacaaa
    18841 tatgataggt cttttttaag aaaaggaaca gataagggac ttgttgaggc tgtatttttt
    18901 tcagacaata catatcttaa aaaaatatta gaagaaaatg atataagtat agaagatgat
    18961 aatttattgg taataaccag attgatatac tcagatggta agagtactgc tagagtaaat
    19021 ggaaggactg ttaaagtgtc tcttcttaaa gaaattgcat ctacacttat agatatacat
    19081 ggtcaacatc aaaatcaagc tttatttaat aaagatactc acttaaaatt tctagacctt
    19141 tttggagaaa atgaattaga agagtttaaa attgcttata aaaatgtgta ccataagtat
    19201 agtgaagtaa aaaaagcttt aaattgtttg acagaaaata aagatgagat gcaaatccaa
    19261 cgagaaatag atttattaag atttcaaata aatgaaattg aagctgcaaa tctaaataag
    19321 aatgaatatg aagatttatt aaaacaaaga gaagtttata gaaatagtga aaagatatat
    19381 aataatctaa attcgagtta tagcaagtta cataatggcg aatataatgt tattgaccta
    19441 ataggtcttg catctaaaga acttaatgac atatctaaat atgacagtgt tttaagtgaa
    19501 tatagtgata ctgtagagag aattatgtat gagttacaag atatatctgg ggagataaga
    19561 aattacaaag ataatataga ctttgaacct tatgagctag agcagattga gcttagaatt
    19621 gatgagatta ataatttaag aagaaaatat ggagatagta tagacgatat atttgagtat
    19681 tatagaaaaa ttaaagatag attagatgaa atattaaata gagatgaaag agttgaacag
    19741 ttaaaaagca aactgatgaa gatagaagaa gatttaaaaa taaaagctag taaattgact
    19801 aaagctagaa tagaagtagc aactacttta gaaaaaatac ttcttgatga attaaaaagc
    19861 ttaaatatga aaaatgtaat gttcaaggtt aattttgaaa aaggtctatt tactttagat
    19921 ggagtagatg atattgaatt tatgatatct tttaacttag gagaagacat taatcctata
    19981 tataaagttg catcaggtgg tgagatgtct agatttatgt tagcatttaa aaccatattg
    20041 gctgatatag atgatattga tacattggtg tttgatgaaa tagatactgg gataagtggg
    20101 atagcagcac agattgttgg tgagaaattg agtgatatag caaagaaaaa acagataata
    20161 tgtataactc atttacctca aattgctgca aatgcagaca ctcattactg tatagagaaa
    20221 gatacttcaa ataataggac gtttaccaat gttagtaagt taaatgaatc tcaacgaaaa
    20281 aatgaaattg ctagacttat agcaggtaac aatataacag aaaaaactat tgaacatgca
    20341 agtgaaatca ttgaattggc taaaaagtgt tagtgaaaaa taagcatttt aataggtgta
    20401 aagaattttt atagaaatgc tttacatcta ttttttatga tgaaattatc ttaaaatatc
    20461 tagttcctat actgaagaaa agtgagttta aaaatggaat aaaatttgta taaatttgta
    20521 actttgaaat ttctaaatgc ttaagtataa tattatttat taaggtagta gtgaggggag
    20581 gcataaaatg aataaaaaag atactttgca cttaattttt ataatcgtaa tttttatttt
    20641 attgtggtat gttgtggtaa cagtaagtga gattaataaa aatattgaaa gttgtagtaa
    20701 caagctagat tatttgtcac aagagattat gagcactaag tcggatatta gtaatactat
    20761 aaatgaagaa atgtcaaaaa gttatataac aaaaagtatt gacctaaaag taaaaaaaat
    20821 cgagaaaaaa gaatgtatta ttgatgtaga tgttcaatta agcaaacttg gagaaaatgg
    20881 gaaagtattt tttatgtaca aagaagagag taataaatgg aatgaaactg agatgacaaa
    20941 acatggagaa ttatcgtatg tatgtgaaat aaagataaat acaggaagtg aatatgacta
    21001 taaagtagtt acaagtggag ctatatctga aagttctgat gtgcaaaaac tgactaaatc
    21061 tgattatatg cctgaacaac ctatatttag ttctgggata agagataata gagaatactt
    21121 tattgaaatg gtagaaaata gtaatctttt taatcatgag agtgaaaagt caaaaaataa
    21181 ggcttataat aatattagat tagaaaaagt agatattatc gtaaatgaag gaaaaaaaga
    21241 tactgtatac aaagctaagt tagttgaaag cttagatgat ggtaaaacta ataattccaa
    21301 tagaaaccaa gtgaattatg aagcaagttt tcctaaaagg gatattgatg acataattta
    21361 tattaaagct aaacttacat acaataatgg agtagaatat acaaaggata taactgaaga
    21421 tataactatg ggattgcaag atttagaaaa ttaaatacat atttaaataa atattcaagt
    21481 ggatttgttc aataaaatag agtataaata aaactgtagt agttttattt atactctatt
    21541 aaaaaattat aattttaatt ccatatatct agcgccttca attggattat aagtataagg
    21601 ttctatatca taaaaaccaa actttttata taatccttgt gcacttttca tgcttggaag
    21661 agtatctaac ctcatgtgtg tatatccaat ttttttagct tcttcaatga tttcttccag
    21721 tagtattttt cctattttta atcctctaaa ttgatttctg acatataatc tttttaattc
    21781 acatatttta ccttcaagtt tttttaaagc aacacaacct gctaaatttt catcaacaaa
    21841 tgccaatatc aatgagcctg aaggtttttt atattttcct ggaagtgttt ttaattcatt
    21901 attaaagtct tgaaaacata agtctatatt tagagaattt gaatattcag taaatagaac
    21961 ctttacgttt tcaaggtttt ctaagtcttt ttcttcaaca aattgtaaag ttatattttg
    22021 agccatttta tccgccatta tataaacatg tttatataat gacttcacct ccttgatata
    22081 tgtatatttt tcttaatatc aattagattt taataccatt actttttcac cgccatagtc
    22141 aagctctcca ttaaactcaa atccaaaatc tttataaagc ttaatagcca ttttattgtc
    22201 ttcgaatata ctaagatata gttcatcaca gttatattcc tttttaagat gatttacaag
    22261 aaaatctagt gatgcttttc catatcctct accttgatat tttgaatcaa tcataaatct
    22321 atcaagccaa actctatcat cttctaaacg accatacata gcaaatccta ttaaagaatt
    22381 ttcatcatat attccaacag gtatccaagc tgttataaat ttagattcag caatagaaag
    22441 tgcattgctt tctatataat ccaattgact tttatctaca gacaaattaa ctaaatcacg
    22501 ccaattattt atgtctgcaa ttcttacatt taatttcaag atattaccgc ccttcaaact
    22561 ttttgttaca atataatact atcatattat gtattaaaaa tcacttatta aggttttaag
    22621 tgaacttttt gaatgaataa attctcagga aaaaggaatt ataaatacat aataatacag
    22681 cataggagta gatggatata tgaacaaaat aaataaaaat ttcaaaaaaa tttggataat
    22741 attaggagct gtatcgattt ttactatggt tataattttc aataaaaatt gtaatgtaaa
    22801 taaagagacc caaatagaag cttttacaat taagaatcaa gataaaaaat ttgtatatcc
    22861 tatgggaaat ataattggag taaaagcaaa tacagacggt gtattggtct taggatatga
    22921 ggaagaggat gtagactata ttggaggcat acaaattggt gataatatag taaaaataga
    22981 taataaaagg ataaaaaata gtcaagatgt atcagagatt ttgaataaga ttaaaaaaag
    23041 taaagttgaa gtgacttttg agagaaaaaa taggtataaa acagaagtaa tagaaactaa
    23101 aaaagaaaat ggcaagtata agcttggctt atgggtaaga gataaaatat ctggaattgg
    23161 aactatgact ttttatgacc ctagtatgga aaagttcaaa gcaatcggtc atgctataaa
    23221 agattcggat actaatgaac tattaaagat aaagcaagga tatatatata agccagaaca
    23281 attgaaaata gtaaaggcta gtaatgaaaa agtaggtaaa ataaaaggag attttaatga
    23341 cagcaattta atgggtaatt tctcaaataa ttcagagcta ggtataagtg gtaatattac
    23401 agaaaaccat aataaagaat ttaatgtagc gaataaagaa aagcaattaa tagaagttgg
    23461 aagaccacaa gatgtaaaaa taggagatgc agtaattctt tttgaggata aaaacaaaaa
    23521 cataacaagc tatgatataa aaatagaaag tatagtgtat gataaaggaa attatagaga
    23581 tatggtaata aaagtagtag atgacaagtt attggaatac acaggaggta tcgtacaagg
    23641 gatgagtgga gctccaataa tacaaaataa taaaattatt ggtgcaataa ctcatgtttt
    23701 tagagataat ccgaaaaaag gatatggtat ttttatagat gaaatgataa aattgtaggt
    23761 gaggcattaa aaaattttat tattttatca attatctagg aggaatataa ttttggagtg
    23821 tcgaatatgc tttagagtag ataattagga agcaattgtg taaaaagttt agttttctgt
    23881 aataagaaga tgttttttaa tggggggatt tttagtggaa aaaatcaaaa tagttttagc
    23941 agatgacaat aaggattttt gtcaggtatt aaaagagtat ttgtctaatg aagatgatat
    24001 cgatatatta ggcatagcta aggatggaat tgaagcatta gacttagtaa aaaagacaca
    24061 accggactta ttaatactag atgtaataat gccacatcta gatggattag gtgtaattga
    24121 aaaattgaat actatggata tacctaaaat gccaaaaata atagtactat cagcagtggg
    24181 tcaagataag ataactcaaa gcgcaataaa tctaggagca gattactaca tagtaaagcc
    24241 atttgatttt gttgtgttta taaacagaat tagagaacta gtttctaata gagttacaca
    24301 agttgagcca aaacctagac cagttcaaga gactcaaatg actagaagtg attttgttaa
    24361 gaatgtagga aatatagaga cagaaatcac aaatataata catgaaatag gagtaccagc
    24421 tcatataaaa ggctatttat atttaagaga agcaataaaa atggttattg ataatgttga
    24481 gcttttaggt gcagtaacga aagagttata tccaagtata gctaaaaaat tcaatacaac
    24541 accaagtaga gttgaaagag caataaggca tgcgatagaa gttgcatgga gtagaggaaa
    24601 agttgacaca ataaatcaac tatttggata tacggtacac aatactaaag gaaaaccaac
    24661 taactcagaa tttatagcaa tgattgctga taaattaaga ctagagcata gtatggttaa
    24721 ataaacagac ataaaaagta aggcttttta attaaagcat tggctataaa tgcgcattac
    24781 aagcagcgaa acggtataac cagtagggtt ataccgtttc gctgttttaa taaatataaa
    24841 aaatttcctt attatttgtt tactatatca atataataat tttattatac tatggatata
    24901 gtatgtgtct ttacaagttg tgaattgaca gtggtttatt ttttaatata aatattgatt
    24961 ttggtgcagg taaactttgt atttttaagc gtattatgga atatgttaaa taaaaaaatg
    25021 atgaaatata gtattgtaaa tgccaaagat gcaaaacaaa cttaaaacat ttattttatt
    25081 gttaagtaat gctataatat aatgtgattt taataatgat agtggaggtt taaatatgag
    25141 agtcgaggcc cctataaaag tagatcgaaa aaccaaaaaa cttgctaaaa gagttgaaag
    25201 tggggaaata gcagttataa atcatataga catagatgaa gttgctgcaa actctttagt
    25261 agaagctaaa ataaaacttg tcataaatgc ggctccttct ataagtggga ggtatcccaa
    25321 taaaggtcca ggaatattaa ctgaaaataa tatactaata attgataatg ttggagaaga
    25381 attatttgaa gaattaaaag aaggagaaac tatagaagtt gtagatggaa agatatatag
    25441 aaaaggaaaa tttttaggtg caggagaagt tttagacaag tatgaggttt cataccaaat
    25501 aaaggctgcc tatgagaatt tagctgtaga attggataga tttatagata atactataga
    25561 ttatgctaaa aaagaaaaag gatttatact tggagaagta gaaattccaa aagtgaaaac
    25621 taattatgct aataaacatg tattgatagt tgtaagaggt caggactaca aagaagatct
    25681 aagtactatg ctttcttata tagaagaggt aaagccagta ttggttggtg ttgacggagg
    25741 agcagacgct ttaattgagt ttggatatac tccagatgtg attgtagggg atatggatag
    25801 tgtaagtgat gaagctttaa aaaaggctag tgaaatagta gtacatgctt atacagatgg
    25861 aagagcacca ggtcttaaga gggttgagga attaggctta gatgcagttg tatttcctgc
    25921 gccaggaact agtgaagata tagctatgct tatcgcttat gagtataaag ctgagttaat
    25981 agtagcagtt ggtacacatt caaacatgat tgattttctt gaaaaaggaa gaaaaggtat
    26041 ggcaagtact tttttagtta gactaaaaat aggttcaaag cttatagatg ctaaaggagt
    26101 taatttgtta tatagaagta aattaaaaat aaagtacata tgggcattaa tagcaacagc
    26161 attatttcct gtgttggtag ttgcatcatt gtcaccagga gttcaacagt ttatacaact
    26221 aatgcaatta aagtttagag tattattaca gatgtaaatt ttaggaggat aaatatgcat
    26281 attaatatga aatattatat agtcactata ggggctatat ttatagcttt gggtataggc
    26341 atgttagtag gttttaattt aaataataat caacaattaa gtgagcaaca agcaaatata
    26401 ataaatgatt tggatgataa gtttaatatt ttaaaagaaa aaaatgataa acttgacagt
    26461 gacttagcca atgtaaatag agattatgaa gaggcagtta attttataaa taaaaatgta
    26521 gataagattt tagctggctc attgaatggt aaaagtatag gaataatatc ggctaatgaa
    26581 aatgatgatt atacaaaaaa tatagaagac ataataaata aatctaatgg aagtattgca
    26641 tttaatatag tgttaaaaga aaatataaca aatccagaaa agttaaagga aatgtcaact
    26701 aaactaggta cagatgtaaa aaatgcaaat gatgcagtta attatataat cgacactctt
    26761 aaaaaagaag atgcaagtga tattttaaca tatcttcaag aactagatgt aataaagttt
    26821 aactctcttg gaaatacata tctaaaatat gattctgttg taatagctgg tggaaatgat
    26881 gcaaaagaca gtacaaaaca gtttgaaaaa atagaaaaat ttgtagtttc aaaacttaaa
    26941 tcagaaaata aatatttagt agaagttcaa aatacaggag ttaaaacttc ttatgtagaa
    27001 ctatattcta aaaataaagt tgcaactata gacaatattg atgaaggtat aggaagtata
    27061 tctctagcta ttcttctaca acaaggtaat atagttggta attttggtag attagatact
    27121 gcaacttcat tgttgccatc tattaagtag aaataacttt aaggagagaa aaaatggatt
    27181 cttatataag tataataata ccagcttata atgaagaaag tagaataaaa tatacattag
    27241 aaagcatatt ggacattaat caaataaatg aaataatagt tgtcgatgat ggttcgattg
    27301 acaatactgc taaagctgta tctgaattag aaaatgaaaa gattaaattt tttaagttag
    27361 ataaaaatag aggtaaagga tatgctttaa attatggtat caagttagca atgaaaaatg
    27421 cagatgttat aggttttctt gatggggatt taggaagctc tgctaaagaa gttgaaaaat
    27481 taatagtacc tatattaaat ggtgaggcgg atgttacaat agccaagttt cctcctgcaa
    27541 aaagaaaagg aggtttggga tttgtaaaag gtttagctaa aaattctgtg tttgagatga
    27601 ctggcgttga gttagatgca actttatcag gtcaaagaat atttaaaaga gaagttttag
    27661 aaaagtttga tgaaatacct tttggatatg gagtagaagt tggaatgacc atcgatatct
    27721 taaagtatgg atttaaaatt aaagaagtat tagtaaatat gacacataat gaaacaggaa
    27781 gaaatttaaa aggttttatc catagaggga aacagtatta tcatataaaa aaggttttag
    27841 gacaaaaaaa gaaagaatgg aggtaatatc ttagtgaaag attatatact ttataccatt
    27901 ttattgttaa ctggattatt tggaacctat gctgtaattc cactatttaa aaatttattg
    27961 ataaatggaa atgtacttag accaaactac aaaaaagata tgataccagt aagtatggga
    28021 attgtatttt tacctatgat tgtaattaat ggaataatac ttgctttctt tactacagaa
    28081 tttaaggatt tagcgtatat atttatgttt atgtttggta tgatatccat gttttttgca
    28141 gggatattgg atgatgtcat tggaaataga gatgtaagtg gtttaaaagg gcattttaaa
    28201 agcttattta aaggaagtct tacaacagga ggttttaaag ctttatttgg tggatttgta
    28261 gggcttatag tttctgttgc tatatcaaaa gacataattg atataatagt aaatacacta
    28321 ataatagctt tatcaactaa tttaatgaat cttttggatt taagaccagg gagagcaata
    28381 aaagcatatt tattcataat ggtaattata tttttaacat tgacaggatt tgttcaagtt
    28441 cttccattac ttatagtacc aaatgtactt gcatacttta actatgattt aaaagcgagg
    28501 gctatgatgg gagatactgg ttcaaacgtt ctcggaatat ctattggaat gctcatttct
    28561 tttgggtatc catttaacat tagattagga tggttaatat ttgtagttat tatgcatata
    28621 ttcactgaga aatactcgtt aactaagata atagaaaaaa acaaactatt aaattttata
    28681 gataaattgg gaaggtagta ttatgataag caaaagagtg ggtattgttg aatcaatagt
    28741 aagtcaaact gaaactctag atgatataag agtaaatata aatggagaaa tacaaagggc
    28801 atataattat ccaaacatat ctggaactat aaatatagga gatgaagtcg ttttaaatac
    28861 aacagcagta gaactgagct taggtacagg tggataccat tttgtaataa ctaatttaaa
    28921 taatatagaa tctactttaa ctgaaggcgg tcatataatg aaacttagat atacacctct
    28981 tcaaataaaa gtagattctg ttgaggaaca agaaagtatt tatcatgata aatttgcgaa
    29041 ttttaaaggg ttagaaggtc tttctgttgt agtagggact cttcatagta tgttaactcc
    29101 atttgtagct tcatttaaaa gaaataatcc aaataaaaaa ttagtatata taatgactga
    29161 tggagcatct cttccaattt atttaagtaa aaatgtggat actttaaaag aaaaaaagct
    29221 aatagatagt acaataacta taggaaatgc gtttggtgga gattatgaat gtataaatat
    29281 atatactgca ctaattacag caaaagaaat actaaaagca gatgtagtat ttgtaagtat
    29341 gggacctgga attgctggga caggaacaaa atatggattt acagggattg aacaaggctc
    29401 tattcttgat gctgtaaaaa aacttgaagg aagagcaatt gcaattccaa ggattagttt
    29461 tgctgataaa cgagaaagac atcaaggtat ttcacatcat agtatgaccg tatttaaaga
    29521 aattgtaaat gtaaatgtag acataccaat aactatatat gatgatgaaa aattgaactt
    29581 tataaaagag caaatcaatt taaatggttt aaacaaaaag cataatataa tttatataga
    29641 taataataaa acgaaagagg atttagacta ttttaatcta aaagtaaaga gtatgggaag
    29701 aaattatgaa caagatgagg catttttcaa agctgcaagc acagcagcat attacttaat
    29761 ggaggtttaa gatggtatta gaagaaaaaa ctattagtag tgatagggtt tatacaggta
    29821 aagtaattac tttaaaggtt gatactgttg aaattccagg gcaaggatat caaaaaaggg
    29881 agttagtaga ggttggtgga gcagtaggaa tagttgcaat tacagatgat aataaagttg
    29941 tactagttaa gcaatttaga aaacctatag aaaagccaat ctttgaaatt ccagctggta
    30001 aactagaaaa aaatgaaagt ccaaaagaat gtgctgaaag agaattaaaa gaagaaactg
    30061 ggtatagtgc aaaaaatgtt aagttaatac ataagttttt cacttcagca ggtttttcta
    30121 atgagataat gtttgtatat ttagcaacag gtcttactcc aggagagaat aatttagatg
    30181 cagatgagtt tttagatgta catgaaattg agcttgaaga agcatataat atggtactaa
    30241 aaaatgatgt tgaagatgca aaaacttcta ttggattatt attagtaaaa gatatgttta
    30301 aaaattaata tttataaaat gaataaccct ttgtcctaaa gcatattatt ttataaagga
    30361 caagggggaa ttaaattgag aagaacatat agaaaaaatg attttagaga attaaataga
    30421 caaataatta ttataggatt gttgtttatg atgtctattg ttgttgggtc ttacataaat
    30481 aaaatattgc caagttctag taacaatata ttaaacaata taaatcctgc tgtagagtat
    30541 tataatttaa atataagcat aaaagatgca gtgatacaaa atttaaaatc agatgcaatc
    30601 tttatgggct caatagctct attaagtctg tttgtagtaa caattccagc agtgttggta
    30661 gcttttgtat taaaagggat gtcaattggt tatacaataa atagttgtat attggcatta
    30721 aaatttaaaa gtataaaaat gattttaata atattattta aaaatttgat tattattcca
    30781 tgtgcaatca tattaacatt aattagctta agttatttta aagaaatggt ttatgaattt
    30841 aaaaagaaaa atagaaaaaa tatgcaattt ttaattaaga ggtatatact aaatattata
    30901 attataatag cactatcatt ggggttgcaa ttaatattaa atactgtaag tattggtatt
    30961 attaagttcc tagctaaata aaggagttta taatggatat tatagaggga tatatagatt
    31021 acataaaaga taaaaaaaaa ttatcagaga atacagtggc atcatacttt atggatataa
    31081 aaaaatatat ggaatatata aatcaaaaaa gcattaaact aatagatata gtagaaaatg
    31141 atattataag ttacttaata aagttggaaa aagataatgt atcaatagct acaatagcga
    31201 ggatgatatc ctctataaaa tcttttcatg attatttatt tttaaatcat atatgtacaa
    31261 ataatcctgc aaaggatata aaaaaaccta aaataaaaaa agaaaatata aatatactta
    31321 cagaagaaga aattgaaaga ctattaaatt ttccaaagtt aacaacacct aaattgatta
    31381 gggacaaggc tatttttgaa gtattatatg gtactggtat aaaagtatct gaattagtag
    31441 agatgaatat agaagatatt gacctagaca ttgattatat atattgtaat tcatgttgta
    31501 aaagtcaaag agtgatacca ctttgtgata ttacaaaact ttatttagaa aagtatctaa
    31561 aagaagcaag acctaaaatg gctgtagaag gtgaaaaatc attattcgta agttcgttag
    31621 gtcaaaggtt tacacgacaa ggtctttgga aagtcataaa aaaatattct aatctagcca
    31681 atatcgataa aaatataaat cctacaatgc ttagacattc atttgcaatt catctgttaa
    31741 atgaaggtgc taacattgct gttgtaagta aaattttagg aaatgttaat ttatctagtt
    31801 tacaagtata tttaaatcat atagataaaa acgtgagaag agaaataaag gaaaaacatc
    31861 ctagaaatga tgttgatata gaattacaaa aagcagaggc taaatagact atatagcaaa
    31921 ttttataatt tagaaggagc atatttgtat gagcagagta atttggattg taattgatag
    31981 tgttgggata ggagcattgc ctgatgctga aaattttgga gatagtaagg atgtaagtac
    32041 tcttggaaat atatttagag aatacccaga tataaatatt cctaatatga gaaaattggg
    32101 tataggaaat atagatggtg tagatttttt tgaaagtata aaaaccccaa taggttgttt
    32161 tggaaagtgt aaagaaatgt ctcagggaaa agatactact acaggtcatt gggaaatgac
    32221 tggtataata gttgacaaac catttaagac atttgaacat ggattttcca aggagataat
    32281 agacgagttt gagaaaaaaa ctggaagaaa agtagtggga aataaaccag cttcaggaac
    32341 tgtaataata gatgagtatg gagagcatca aataaaaact ggtgatgtaa tagtatatac
    32401 atcagctgat agtgtatttc agatagctgc taatgaagaa gtaattcctc tagaagaact
    32461 ctataatatg tgtaaaatcg ctagggaaat aatgatgggg gacaatgcag tagctagagt
    32521 aattgctaga ccattcatag gtaagaaaaa aggtgagttt gtaagaactt caaatagaag
    32581 agattattca ttagacccat ttgaaccaac agttcttgac aatataaaag aatcaggtct
    32641 tgacgtttta gcagttggaa aaatagaaga tatttttaat ggaaaaggaa ttacagatgc
    32701 tatccacact aaaagtaata tggatggagt agatgaaact ttaaattata tgaaacaaga
    32761 taataaaggt ctgatatatt caaatcttgt tgattttgat tctaaatatg gtcatagaag
    32821 agacccagaa ggatataaga aagctcttga agagtttgat agtagacttc ctgaaataat
    32881 ggctaatatg agagaagatg atattttaat aatcaatgca gaccatggaa atgacccaac
    32941 atataaaggt acagaccata caagagaata tataccagtt atgatttatg gcaataagat
    33001 taaaaaaggt tttaatttag gagtaaaaga tacttttgct gatattggag caactgttgc
    33061 agatatacta aatgtaaagt tgcctaaaca tgggtcaagc tttaaaaaag atttatttta
    33121 aattagaaaa taacaaaaac ttagtttaat aggatggaga gattggaatg tttgaaaaga
    33181 tagcacaaag tagtaaattt ataaatgcta aaattaatat aaaacctaaa attggtttaa
    33241 tattaggttc tggactaggc gatttagcaa atgatataga agactctgtc acaattaaat
    33301 atagtgaaat tccaaacttc cctatatcaa cagtagcagg acatgctgga caacttgtca
    33361 ttggaaaact tgaaggaaaa gaagttattg ctatgcaagg tagatttcat tattatgaag
    33421 gatattctca aaaagaagct acttttccag taagggttat gaaagaatta ggagttgagg
    33481 ttcttatagt aaccaacgct gctggaggtg taaataaaga atttaaagca ggagatttga
    33541 tgcttataag agaccatata aactttagtg gaagtaatcc attagtagga aaaaatgatg
    33601 acagacttgg tgatagattt ccagatatgt cggatgcata ttcttctaga tatgtagatg
    33661 tagttaaaaa ctgtgctaaa gaatgtaata tagatgtcaa agaaggcgtt tatatgtttt
    33721 ttagtggtcc aaactatgag acaccagcag aagtgagaat ggcacaaatt ttaggtgctg
    33781 atgctgtagg tatgtctact gttccagaag tcatagtagc ttcacactct aatataggag
    33841 ttattggtat ctcctgtata acaaatatgg ctgctggcat cttaaatcag tcactaagtc
    33901 atgaggaagt aatagaaact actcaaaaag ttaaatcaga atttatgaat ttagttaaaa
    33961 caacagttaa aaatttataa gttgaagatt attataaatt ttttgagcaa atttataata
    34021 aaatagttaa tgtttaattt aaaaggatta ataaagagct gtcttgatat agaaagttat
    34081 ttctaataag ggacagtttt tttataatac tacaacattt gaaagtgttt taaagtttaa
    34141 tatttattta aattattata ggttagaatg gagatttcaa ttatgagaat gtatgatatt
    34201 ataagaaaaa agagagataa cctagagctt agtaaaaaag agataaacta ctttatagaa
    34261 aattattcaa agggagatat acctgactat caagcttctg cactattaat gtctatttat
    34321 ttgaataaga tgaataaaca ggaaactgta catcttacag aagcaatgat gaactctgga
    34381 gacatgataa acttatctga gatagatgga ataaaagtag ataagcatag tacaggtggt
    34441 gttggagata agactactat agctcttata cctttggttg cttcatgtgg agcaccagtt
    34501 gcaaagatgt caggtagagg tcttggtcat actggtggta cgattgataa attggagtct
    34561 ataccaggtt tctcgacaga aatggagata agtaaattta ttaattctgt aaataagtct
    34621 aaaatagcgg tttgtggtca aagtgcaaag gtagcaattg cagataagaa aatatatgct
    34681 cttagagatg ttacagcaac tgttgataac atatcattga tagcatcaag tgtaatgtct
    34741 aaaaaattag cttcaggagc tgatgcaatt gtgttagatg taaaaactgg tgatggagca
    34801 tttatgaaga cgttagaaga gtcttttgat ttagcaaagt ctatggttga tataggcact
    34861 ggtatgaata gagatacaat agggattgta acagatatgg atgaaccttt aggttttgca
    34921 gtaggaaact ctttggaagt tatagaagca attgagactt taaaaggaaa tggtcctaaa
    34981 gaccttgttg agctatgtga agtactagga gcatatatgc tggttttatc aaaggtagca
    35041 tatgattttg atgatggtgt tgaaaagata agagaatcga taaaatctgg tgacgccata
    35101 cataaactca aagagtttat agagaaccaa ggtggtaata aagatgtagt agatgattat
    35161 tctttattca aaaaatcaaa gtataaatat ccagttaaat caactaaatc aggttatata
    35221 aataaaatta aagctgaaga tgttggtgta tcagctatga ttcttggggc aggtagagaa
    35281 actaaagatg atgttttaga tttatcagtg gggataattt tagaaaagaa agtaggagat
    35341 tatataaatg aaggtgaagt tttagcgtac atgtattata atgatgaaca aaaactttca
    35401 ttggcaaagg acagatttat tgattcctat tctattgtag attcgaaggt agaaaaaaat
    35461 aaactaatat acggtattgt aactaaagaa gggattaaaa agttttagaa aattttagaa
    35521 aagtgattat aaaaggccta acatgtcaaa aataatgtta ggttttttat tttttgataa
    35581 gttgtaaatt ataccaaaaa tatatatttt aaaacatttt ttacaatata aataagttta
    35641 ttctacaaga tgattgaaaa ttaaagtatc tagctttata atagaaaatg gataaatata
    35701 cataaggagt agattatatt atgaacttta aagaaaatag attgaaaata gcagcattaa
    35761 taattgttat tttgattatc ttagcaggta tatttgtgtc tatgcaaata ggtccttata
    35821 ataaaaataa caaaaaagat gtaattattg acattccaaa tggagcatct attggaaaaa
    35881 tatctgatat attatatgaa aataaattaa taaaaaatga actattattt aaacttttgg
    35941 taaaggttag caataaagct ccatctataa aatcaggaac atacttactt aatcaatcgt
    36001 actcaaataa tgatataata agtcttttag tttcaggtaa aatatatcag gatggtataa
    36061 aagtgactat tccagagggt gctacatcta aagaaattat agcaatgtta gtaagtaaaa
    36121 atttaggaga taaagctact tttgaaaatt taataaaaaa acctcaagaa ttttatgata
    36181 aatttccata cttaaaggaa gatggaataa cttctttaga agggttcctg tatccagaaa
    36241 cttattattt taattcaaag aaacaatcag aagaagatat tttaagcgaa atgttaaagg
    36301 tatttgacag taaatacacg gacaagttta aaaagaaaca aaaaaaatta aatatgactc
    36361 ttcaagaagt aatggaaatg gcatcaataa ttgaaaaaga agctgttctt gacaaagata
    36421 gaccaataat agcaagtgtg ttttataata gacttaaggt aggtatgcct cttcaatcag
    36481 atgcaacaat acaatatata tttgaagaga gaaagaaaat tgtaacgtat gatgatttaa
    36541 aaattgattc accatataat agttataaaa ataagggatt acctcctaca ccaatatcaa
    36601 atcccggtat aaaatctatt gaagccgcgt tatatccaga taaaacagac tatttatatt
    36661 ttgtagctaa gattgatggt ggaaataatt atagtacaaa ctatcaagac catttaaaat
    36721 atgttaaaga atataaagaa gcaagggata aacaatcaaa agatacaaaa gcaacaaata
    36781 aagaaaatac aaaaaaataa accatgatta taaaaagcct aacagggtaa gaataatgtt
    36841 gggctttttt attgaaaatt gttattctta gatttataat aaagaaagtg taaatatata
    36901 caacaatatg aatttaaaaa ataataaatt aatataaaac ttgaacattt attattttag
    36961 ggagtgtttt tatgagtaat atagttaatg atttggtcga agattatata agagtgacat
    37021 taaaagaaaa agaaggattt ttaaaagatt tagaaattta tgcagaggaa aatagtgttc
    37081 ctataataca taaagaagtt tcagatttat taaaagtctt attaaagatt caaaaaccaa
    37141 agagagtttt agaagtagga tgtgcaattg gttattcttc tatttttttt gcaactatta
    37201 taggaaaaga tgcagacata ataactgttg agagaaatga aaaaatgata gaaaaagcaa
    37261 aagaaaacat aaaattagct ggatttgaaa ataacataac tatactagaa ggagatgcag
    37321 aagaaaaact tagtcaagtc caaggtgaat ttgaccttat atttatagat gcagccaaag
    37381 gtcaatataa gctatttttt gatttagtta tagataaatt aaaagatgga ggacttttag
    37441 tatctgataa tattttatat aagggtatgg ttgctcatga tgattttgtt gtaagaagaa
    37501 aaaagactat agttaaaaga atgagaaatt atttagatta tatatgtaat tgtgattatt
    37561 taagcacatc attgatacca ataggtgatg gtgtagcact aagttataaa gaaagtaaaa
    37621 gaggtgatgt agatggaatt aaataaagta gaacttttgg ctccagcagg tgatttggag
    37681 agattgaaaa tagctataac atatggagca gatgctgttt atataggtgg agaaattttt
    37741 ggaatgaggt ctgcagcgaa gaattttagt aaagaagata tggcagaagg agtagctttt
    37801 gcacatgaaa gaggaaagaa agtatttgta acagtaaata taatacctca taatgaagat
    37861 tttctacaat tagaagacta tttgttggaa ttagaagaga taggaataga tgcagttata
    37921 atagctgacc caggagtact tagtgttatt aaaaaagtaa ttcctaatat ggaaatacat
    37981 ttaagtacac aagcaaatac gaccaattat ctaagtgcga acttctggta cgagcatgga
    38041 ataaaaaggg ttgtagtggc aagagaatta tcttttgatg aaatatctga gataagagca
    38101 aaaacacctt tagacatgga tatagaagcg tttatgcatg gtgctatgtg tatttcttat
    38161 tctggtagat gcttaataag caactatatg actggaagag atgcaaacaa aggttcttgc
    38221 gctcaatctt gtagatggca ataccactta gttgaagaaa aaagacctgg tgagtatttc
    38281 ccaatatatg aagatgaaag aggtacattc ttctttaact caaaagattt atgtatgata
    38341 gagtatatac cagagttaat caaatctggt ataacaagtt taaaaataga aggtagaatg
    38401 aagactgcat actatgtagc tactgttgta agagcataca gaatggcaat agatgaattt
    38461 tacaaagacc cagaaaactg gaagttcaat ccaatgtgga tggaagaact aaaaaaaggt
    38521 agtcatagac attttacatc aggtttttac ttgaataaac caacaacaga agaccaaaat
    38581 tatcaatcag catcatatgt tagaaactat gattttatag ggatagttag agaaacagaa
    38641 gatgaagatg gtcttattgt tgttgagcaa agaaataaaa tgtgtgttgg tgatgaaata
    38701 gaggtcatgg gaccatataa agaaactatg tttactaaaa ttgaagcaat gtacaatgaa
    38761 gaaggagaag ctatagaatc ggctcctcat cctagacaaa tagtgaagtt aaaactatca
    38821 gtaaaagttg gtaaagatta tatgcttaga aaagttatag aagaaaaggt tgaagaataa
    38881 taaaaaattg ataaaaatag tattagcccc ttagaaattg tcaataatga aattatctaa
    38941 ggggtgattt catgtcaaaa aagaagactc catttttgaa aaaagtggga aagagaagct
    39001 ggtgtgtatt tactataatt ttaataattt atagtgcttt aatttatagg ctagttgata
    39061 tacaagtatt aaaaggtgat aaatacaaac aaagtgttga atcacaaagt gttgagaaag
    39121 tcgagctaaa cagtggaaga ggaataatat atgatagaaa caacaagaag cttacagata
    39181 caagtaagta tcaggtatta gttgttgaaa aagaaaaatt aaataataac tataaaattt
    39241 tggagcttat taagaaagct actaaaatga atgatttaga tatatataaa gctgtacaag
    39301 agcaattgac acgccctata atacagattc aggctaaaaa tatcgataaa aatatgaaaa
    39361 aagaacttga gaaaaatgga ataatgatag aagaaaaaac tatgagatat tcaaaagatg
    39421 gtttattatc acacacaata ggatatataa aagaagatga taaatcaggt cagtcaggaa
    39481 tcgaaaaaag tatggacagt gtacttagaa attcaaatga aaagtatata agtgctttta
    39541 aagctggaga tgcaggaaat gaaaaatctc taaacatact aaagggaagt gtaaaaacta
    39601 ttgataataa agataaagat agacatctaa aaactactat tgattataat attcaaagga
    39661 aattagaaca aatattaaat aaagaagaaa atcctacagc agcaataatc tcggaggtat
    39721 ctacaggtga aattcttgca atgtgttcta gacctaattt tgaccaaaat gatatatcaa
    39781 aaagtttaaa aggaaaaaat ggtgaatttg aaaatagagt tataaaggcg acatatccac
    39841 cagggtctgt atttaagatg gtagtcttat tttctgcact tgaaaatggt gttatagatg
    39901 aaaactatac gtacaactgt acaggcaaga ctaaagttgg gaatactaat gaaatactac
    39961 gatgtaataa aagagatggt catggtttcc aaaatttaag acaagctttt tctaattcat
    40021 gtaatcctgc atttttagat atagctatga agcttggaaa agaaaagata cttaaatctg
    40081 ctgaaaagtt gcatttattt gaaaaggttg atattggtct tgatgaagaa aaaattagag
    40141 aagctccaaa aaacatatca ataagaaatt tagctatagg tcaagaaaat atagaattta
    40201 caccacttca aataaatcaa atgactcaaa taatggcgaa caatggaaca ttcaaaccat
    40261 tatacttata taagagcctt gtggataatg atatgaatac aataaaaact tataaatcct
    40321 caaaaaagga agaacttatt tctccatatg tatgtacaca agttaaagaa tatatgaaat
    40381 ctgtgtctag gactggtaca gctaaggacc taaaagatat tgagggtgga tgtggggtta
    40441 aaactggtac agctcaaagt agtttgaata aaaaagcaat agaccatgga tggataactg
    40501 gattttatcc tgaagaaaga cctaagtatg taataactgt attagtagaa ggaactcaaa
    40561 aaggaaataa atctgcaaca cctatattta aggaaatatg tgaaagtata caataaaatt
    40621 atatactata aaatttaagg atatatcgta atcttgattt tagatagaaa gttttgtcta
    40681 gtgatgtacg atatatcctt taaaaatgta gtattaactt tcttgaacaa gctctttttc
    40741 tagtttttta agagcttttt tctcgattct agatacatat gacctcgata tatcgagttt
    40801 ttgtgcaatt tctctttgtg tcttatagcc ataaggagtt aacccatatc ttaactggac
    40861 aatttctctt tctctaggag taagaatttt gtttagttgt tcgtaaagct ttccaacttg
    40921 aacctttagc tcaacttcat caagaacata gttagcttct gtgccaagta tatctaaaag
    40981 acttatttca ttacacacta ctctttatat gttattggta ttactagtct tcatgtaatt
    41041 tcaaatgaac cattaattca aaagaatcca atttttgttc tttagactta taataaactg
    41101 ctttatcaat tacagatttt aggagtttat tttttccaag ggaatctgaa tcatggtaaa
    41161 gtgataacac agttttaata tcttctatta ttttagcatt gtctttttta acagtactca
    41221 atctattttt tactgtctta attgaatctt taatttcttc agttcttgaa cttatattgt
    41281 tggacctttc aataaatgta tcttcatcat atattccacg ttctaataaa tcatataact
    41341 tatttttttg cttactcaat tcttttaatt cagtttctaa accctttaag actcgctttt
    41401 gaaattctat attactgtct ttattatttt tatctaagtc tatgtcagat gtttttaatt
    41461 gatatttttt tacagtgtct tccaaagatt ttaaaatagc agcttcaata aattcaaatc
    41521 gagaactttt attgcaacca ggatggtaac aaattatata gttgtggtct gcatatggtc
    41581 tatatactaa tggcctacca caatttttac aaatcactat tcctgcaagt ggatttttta
    41641 tttgattacc atagctgaca tgaccatttt ttttgagtat attttgagcc tttttccatg
    41701 ttatttcact tattaaaggt tcatgctttc cttttgcttc aatccattca tctttaggtc
    41761 ttaatttaac tgtttttatt ttatttggat ttttagattt tttatagtcc tttttttgcc
    41821 actgaatgta gccacagtag actttatttt ttattatatt tgtaattgca gaattgtacc
    41881 aattagcacc tgtagcagtt ttaatgccta ggttatttag gtattttgat attttactac
    41941 aacccatatc ttcattgata tataagtcaa agatagtcct aactatatca gcatctttat
    42001 ttggtacaag tattctttct tttctaccag ttgttacagc atcatatcca aatggagcag
    42061 aagttcctat gaaattccct tcttcaactg attttattct acctctttgc atccttcttg
    42121 atataagttt tagttctttt cttgccataa atgcttcaaa ttctgaatac tcttcatcaa
    42181 attcatctgt taggtcgtat gtttttctag gtgtgatgat tttggtctta ctttctttaa
    42241 atgtttctag tatgatacct tggtctttca tgtctccacg accaagtctg tctaagtcca
    42301 ttactaagac tgcgtcgtat ttattttctt cgacttcttt tagaagttca agcattttgg
    42361 gtctatgtat tatactttca ccagaaacta attcttcttt tatctcaatt acatctaagc
    42421 tttgttcttt tgctagtttt agtagggttg atttatgcct acttaaagtt tcaaattcgc
    42481 cttgtttttc tgcttcttca tcagcacggg attttctcaa gtatatggca acactcatat
    42541 aagtaatcac actccttagg tactattata tattatatta aggatataat gggataattt
    42601 atgctaatta aaacttgata taattgtaaa aatagtatga aaatttagca tgaatcgaca
    42661 gcattcaatg agaaaatata gtataatgca attgagaata atttttaggg ggtaattata
    42721 tgtgtaattt taaaaaaata ctaggcttgt ttatgataat tatgttatca gtttctatgg
    42781 tagcatgttc tagtggggga gattcaaaag atgaaagcaa aggtaaggac aagcaattta
    42841 ttgaggattt aggaaaaggc ttaaaaaaaa gatgggattt ggcatctaaa gaagaagctg
    42901 gaacactaaa cttagagcag aatgaagctt atgaaaaatt tgtagaagta gaaatggatg
    42961 ctgtatcaaa atataaagat gctgagtttg atagcccaga acttggcaag ttggcaaaag
    43021 attatataaa agctcttgaa gattcaaagg aagctataaa atattattca aacgaccaat
    43081 taaaattttc agaactttgg gaaaagcctt ataatgagag aagcacgata ttgttgactt
    43141 tagttgataa gtttggtttg aaagtagatg aaaaagagtt taaagtatta aaagacaatg
    43201 cacaaatcgt aaatgaagaa aatgatttaa aagaaaaagt taatgctatg gcaaaaggta
    43261 taaaatttga aaaagtaaaa gatgaatatg attggaagac ttatcaagct actgtagaaa
    43321 atacaagtga tttagatctt caagattttt acttagatat aaatctaata gacaaagatg
    43381 gtgtaataat cgagacacaa acatcaacat cagtaaatgg aacttggaag aaaggtcaaa
    43441 aagttagact tgagtttagt acagataagg attttgaaaa aatagagtgg acacctcatt
    43501 attctgcaaa taattatagt ataactgaat agataatttg tatttaaaaa tatttagaag
    43561 gggaagatat tatgaaaatt aaaaaattat taacaatagg gttatcttta tctatgttta
    43621 ttgctagttg ccatatatct gcaaatgctc ttgataaaat tgaatcaatt aaaggtgcag
    43681 ataaatacga aactactggt ataattgcag ataaacaaaa ctatactaca gctatattaa
    43741 ttaatgcaga tagtacaatg gctgatggat taagtgcaag tggacttgca ggagctacaa
    43801 acgctccaat tcttttgact aagaaaaata acataccaaa tgctacactt aaacgattag
    43861 aaaaggcaaa aaaagtatat ataattggag gagaaaactc tattgataaa tatactgaaa
    43921 ctgttctaaa aggtaaagga atagagatta aaagacttca aggtagtgat agaattaaga
    43981 caagctataa tgttgcaaaa gaaataaatt caattaacaa agtaaataaa gtgatattga
    44041 ctaatgcttt taaaggagag cctgatgcaa tgagtgctgc acctgtagca gttagagaga
    44101 aagcagctat agtattaact gatggaaaaa gtgttccatt taatacaact ggtgtagaaa
    44161 gttatgtaat aggtggtact tcatcaatga atgacaaact agttaatgat actaattcaa
    44221 ctagattagg tggcacagat agatatgata ctaacaaaaa gataataaat aaattttatg
    44281 gtggagtaaa agaattttat atagcaagtg gtactgattt agtgtatgct cttgttggtt
    44341 ctactgttgc taaaaataat gctattgtgt tagttaataa tgatagtaat aaaagtgtat
    44401 taaaaagcac aactaaatta actgctatag gaaatttgag tgatagtata ttacaacagt
    44461 gtttaaatgt aacaaaaaat ataggagatt ctaatactgg aagacaatat acagtagaag
    44521 atgctgctaa tgcagtaaaa aaactgctag gtgtgaagaa attttattat aatgaaaatt
    44581 atccagaaga tgaagatgta atattcttac tagatgagaa ttttgaattt tcaggaaaac
    44641 aattctatgc atttggttat gttacagaaa attctgaagg ggatgttaga tactgtgtag
    44701 agaaaaatga tatgagcaaa gtttatacat ataatgttag tggagttatg caagtatgta
    44761 acccagatga ttataaaaag aaggaaatga tatcagaaga aaaagcaaaa caattggcat
    44821 tagaagagtg tgccagaaaa cataaagttg gtgttgattt attaaaagtt aatattgctt
    44881 atattgatga ttttgatgat ataaaagtat atgctgtaag aatagattca aaaccacatc
    44941 caaatcaaac ctatggatgg tattttatag atgtttatag tggatatgct tataaggatg
    45001 tttggaataa catttaaata gtgtttttaa ttatataatt taccaatata tgttataata
    45061 attttagcaa gaagatgtaa tctacaatct atagagtgga gttcatacaa acaaaggatt
    45121 atcctcccca ttttaagaag ggaggtgatt atgtatggat aattttttac ttagtatatt
    45181 agccagtatt ctggctagct atatagttta cttaataagt aagttattta gaaaacgtaa
    45241 aaaaccactc aaagctggca ctaagagtgg ttgggaactt gattttaaaa taaagttcca
    45301 taaattcaaa taacatcgtt tatgatgaac tccactctac tgctaaatag attgtagttc
    45361 ttcttgcttt tattatacca caaattggtg cagatattca aaaataatat atttataata
    45421 taataaaatt atagaagttt tgcagtgagc gaaatttgtg ataaaatatg gcttaacaat
    45481 tggaatataa tgcattgaag atgtgtgata agtattatca attgcactac tcatggttca
    45541 ctgcaaattt aagagagttg tatagatgta agtattggaa atactcaatt tgttttgggg
    45601 ttttatatta actaagtggt atgtaaagct caaaattaaa tagttggcta tggttaaggg
    45661 attgccgttt tatattaact aagtggtatg taaagataat agcagatatg atgtttaata
    45721 acggaagtat aaggttttat attaactaag tggtatgtaa agctgaatga acctaaggta
    45781 gttgaacaga ttgaaaagac gttttatatt aactaagtgg tatgtaaagt atgtcaggac
    45841 ttcttaaacc atattcagca tacactcgtt ttatattaac taagtggtat gtaaagaatg
    45901 ctgaacttaa agtaatgatg ctattttggg acagttttat attaattaag tggtatgtaa
    45961 agaaatttgc aaggctaaat tgccccttaa ttaatggtgg ttttatatta actaagtggt
    46021 atgtaaagag tttttctata tattcagata tttcactttt atcttgtttt atattaacta
    46081 agtggtatgt aaaggaaata tgtatagaag cattgaaaca agataaatat tgttttatat
    46141 taactaagtg gtatgtaaag tttatattcc tcacacatat aatacaatta attcagtttt
    46201 aattgtcatt atgctcaata tttagttttt ctgtatctta taaaccaaga atataattga
    46261 tagagacacc aaaaatttta gcaaatatta ttaactcatc atctcttatt ggtctttcat
    46321 cagactctat tatattcata acacttgtat ttatatttgt tttttcaaac aattattttt
    46381 gtgacctatt catattttcc ctaatatatt taatcacttg acttatgttc atacatgatc
    46441 acctcgaaaa atttctattg tagagttgta aaaaatttag ctttaaaata tagaatatca
    46501 agtgatttta agtaagaatt gtaacaatta aataagtata agacaagttt atgagttcat
    46561 attttataac aaggggtgat gttatgaaaa tactacaaca atttaaacca tacaaatatg
    46621 ataaagaaaa aaatatagaa tatgatgaaa aaattatagc taatcaaaac acagaagttt
    46681 atatagtata tccacgtata tctgatgaag aaaataggag aagatgggaa gagttcaatg
    46741 aagtagctaa agaagtagcc attaatatag ctatgaagaa agttgagaca gtagaaagtt
    46801 aagagaatag agaagttgct aatacttagc actttctcat agaagaaaat tggacaacta
    46861 tttttaaata actaggaata aaaaagttaa atcatatttg aaaaaaggtg ataaaattgt
    46921 tgagtattgt tattagtaat aagaatgtat taactattaa aagaaactat aagtttttag
    46981 taaatatagt atttaaggat attataaact ctgcctgttt ttcggggtct ggattgttcc
    47041 ttgcattgtt tatcgttgtc tgcatttcta ctatggcttc tgcaacagaa atgccatgtg
    47101 cacgggcaac aatctttaac aggtattttg tatctcgttt cttaaatcca atcatatata
    47161 tgactcattt cttaatttga ccatttgcaa ccataactca tggtgtgatt tattttatga
    47221 cacccttata agagaaacta taataaatga gaatgaataa aaccgtatcc tgttaatcac
    47281 atgtttaatg tgaaatacgg ttacggtttt tcttcgttga atttatagcc aacaccccaa
    47341 acagtcaata tgtactgtag attatctgag gacggctcta ttttctatct tatttcttaa
    47401 ctttcgtata aatgatgtca gattgctacc ctctggcgca ttattgaagc tccatatgtt
    47461 atcatatagt tgttatttcg tgaataccat tcctttatgg caaaagagaa aatacatcaa
    47521 atcaaattct ttagcagtta tatatataat ttcccctttt tcccacctac tttatagcaa
    47581 acaaatctaa atattcttaa gtaacataat ctttatataa tacatattcc taataacaat
    47641 agccagaaag cacttaatga tgaaaaaacc ctattaacta tgaacaattt tttaatacct
    47701 aaaaattcta agttgccgat aaaaagacca attgctatga ttatacctaa aagcccaatc
    47761 caatttgcta cataatttat agacagtaaa ctcattgaat tacagaaaat cataaaactt
    47821 aaaaatacaa atgcaacatg ctctgtcatg gtttcaccta tatatgtgtt taatactttg
    47881 tataattgtc cagcttcttt ttcagtaatc tgtttattta tcatctggtt agacaacatt
    47941 ggaaaggtct ttgtatatct caagattcct acgaatagaa gaactccgta gattattcca
    48001 ttgatatatg caaagtttga ccaaataaat tctcccttac tttgttcaat aaaacgtaat
    48061 gcaatagatg cctggacaac cataaaacca ccaattccaa atagatagaa tagcaaagaa
    48121 atcatagttt tcttattctg cattctcttt agaattataa taggattttc tctaataatt
    48181 ttaggataac caaaactaaa cattaaacct atcatacttc cagcaataca aaaagctgat
    48241 aatattgata aaacacctac aactatattc atattttcct cttttctaaa ttatattttt
    48301 taaatatatc aaagtaataa aactaactta ttgtcttaaa atttctagaa tatctctcaa
    48361 tatgccgttg aacttttcta tctcttcttg actacatttt gataaaactt gataaatctg
    48421 aattttcata ttcatttcct gctcattgtg attatgataa atttgttctc caagagtagt
    48481 taaatagata ggaattgtac atccgttacc ttgttctcgt cgcatttcaa ttaaattttt
    48541 ttcttgtaaa tgtgcaataa tgacggacat tcttcctttt gttacaccta cttctttaca
    48601 tagttctttt tgcagaatac ctggcatttt tccaatcatt acaagtgtat gaatctctgc
    48661 aatatataat ttatcatcag ttccatagga ctttgaattt ttattacgtt cagaagccca
    48721 atttaaaatc tgtacaaaag tattaaatat ctcatgttct tccatttttg ttcacctcta
    48781 aatacagttt accccgtaaa ctgtaagagg tcaagtcata tttgatagaa cattctatta
    48841 ttataaaaaa catgtaaata agggttactg ctatttagat gtagaagtaa ggttttatat
    48901 ttatcttact ttaacatctt ccagttgcta tctttgtgaa tcctcaactc aaactgtgaa
    48961 atctgtgtca cctttgtctg attatcaata aacttcactg acaagccgat tttcattgtt
    49021 cgtgagtttt tctgttttat tacttttttc tcgttctctt ttttcttgaa catgatttca
    49081 attcctttct attgtttgat acgtcctgca cctattaggt gctgttgcaa gtaggcactc
    49141 gtaaggtctg catagccaat cggattgttc acatggtaca tatggttgtt gccaatgtat
    49201 attccgatgt ggcttatata gctaccgttg ttataagtag agtggaaaaa catgaggttg
    49261 tcagcttttg cctgtaaaag cgggatgagc tataaaagag aaagattatt taaaagttga
    49321 gaaaaatagt ttctttagtt ttattcataa caattcattt aagggtttta tttcagcact
    49381 acatgatgat gacgttttag atagtaaaag cttggacaaa ttagaagaat attttaaaaa
    49441 ttttcaagaa aaatgtattg atgactaata aagctatcag tatcttttta gtttgagaaa
    49501 attgagaata gtgttttgat ttaatgctag tgttatgtaa tggtagaaaa ttatataaag
    49561 aaaagtgctt atttccttgt atttgatagt aaaaaattta gaaaataata ccgattaaaa
    49621 tttaaagaaa tatatgaaat actacaatag tttaaatcat gcaaatatga taaataaatt
    49681 gaaattataa aagctatata attttagaag aaatagttga cagataaatt gccttactat
    49741 attattgtat taaatcatta atacaataat atagtaagga ggtaatgaat gaaatctaaa
    49801 aaatacaata ttacaaaagg attatttata gtttacataa ttattttgac atggataata
    49861 ctattcaagt tacaatttga tgtttcatcc attgaaacca tgaatcttag aagcataaac
    49921 ttagtacctt ttgctgggtc acttataatt aataataaag tagatatatc agagattata
    49981 ttaaatgtgg ctatatttat accatttggg atatacatat gtatgctaaa agaagagtgg
    50041 agctttataa aaaaagttat acctattttt ataactagtt tggcatttga aacacttcaa
    50101 tatatatttg cactaggtgc aagtgatata accgatttga taggaaatac actaggagga
    50161 atcataggaa tagctgtttt tatgctattg tcgaaaatat ttaagaataa taccataaaa
    50221 attattaata tattagcatt tattgtgaca attattgttg tattattttt aggcttagtt
    50281 atttttgcaa acttatagaa ataagtatat aggtttaact taaaatagag cgtaaaatac
    50341 agttacatat aaacttattt acacataaga agcacctata tagacatata agtgcttctt
    50401 aaattattaa agaattattg tatcttgaaa ctcttgtata ttcaattctt gattttctct
    50461 tgttttttct aataaatcta acgcacaatt tatacaaact ttaatttctg cattggtatt
    50521 tttttcataa taagttatat atttgtttct atatttatct atttcttttc cacaacgact
    50581 acacattaca attacgctca tagcttagta cctccatctc taattattct taatatattc
    50641 aatagtatca cttttctgaa aataatgatt gttaaaactg tatcaaatat agatttactt
    50701 tttattataa tgcattttaa taaaaaaata aaggttatga gtaaaatatt tatagaaaaa
    50761 agtatattat tgaattgatt ataaagtttt attattttta tcattcttaa atacacatta
    50821 atatctataa aatgacaaaa taaaatatac tatttaaata gatattaaaa aattgatgcc
    50881 aagtatttgt ttgctatatg aataacaatt tatttaggag ctttgatttg acactgtata
    50941 atgataacgt aattgttaaa aaagtttgct gatttggatg tgtttttaga aaatagaaaa
    51001 atatatactt actgactaat gataatttta tatgtatttt agtttgagtg atttaagaaa
    51061 agtgttttga agggggagta ttttatagag taactaaata tatataatag tagaaaataa
    51121 catataaaaa gtagtgtgta tttcattacc ttctttatct gttccgatag ggtcttgtag
    51181 agatacttga gttttatttt ttttgttaga cctaatattc attaaaattt cattttcaat
    51241 acatctagaa gcatatgttg ctaatcgagt tcctttatct atgttataag tttctatagc
    51301 tttaattaat cctattgtac ctatagatat taaatcatcc tggtcttctg ttgaattatt
    51361 atatttcttt gctatataag ctactaatct catgtttctt tcaattaatg tatcttttgc
    51421 acttttatcg ttttctattt taaatttagt taaatactca atctcttctt caggggttaa
    51481 gggtttttca aaagatttaa gagctgccaa taggtcaccc ccttttcaac ggtcacttta
    51541 aattatatga aggagactta tccaagttgc ctgttatcta ataaaataat atgcatattt
    51601 ttagaattta aaacatatat ttgcaaactt ttagatggaa tatgacataa agagtgggaa
    51661 tttaaattta agtttttgtt gtatatatat gaaacaaggc gaaggaatta ttgatttata
    51721 aagtataaat tgaaactgag gtgtcaataa atataaagga aatagtattt atgtatgttt
    51781 tgatgttgag gtaaaaaatt aatgaaaaac attagaatct ataaaaaata tgaatatagg
    51841 aggtgtaatt ttgcaataat taaaaaagta aatgcaatta attatttgga taaagttgat
    51901 tgcaagtaaa aagtattagt catagttaat actgttaagg gtgaaggtgg tgtgttaatc
    51961 tgtatgtgtg taggaagagt tttgagttat aatgaaaaaa cttataaaag gtatttatga
    52021 aaaacttata gttaaccatc tatatataaa atattattta ggggttgttt ttattattga
    52081 aaataagaaa gcgtggtttt atatcaatcg aatgtgtaat aagtattgct atattatatg
    52141 tggctgttta tttagtttct acatcattgt atagttgtta tagttttgtc agtagaaata
    52201 tatctgacag aaaaatgtta agtactgcaa aaaaatatat agaagatgag aagtatagaa
    52261 tacaaaatag taagtatgag ttaattgaaa ataagataga aaaaaattac ataaatggat
    52321 atgaaattag cagtagagta gagcaaattt tagattatta tcaatgctat gaaataaata
    52381 tagagataaa aaatgaattt aaaaaattga ggtttaatag ctatgttact agaaaataaa
    52441 ggctccatat taataataac attgataata ttctctatta taagtactgt gtgtattatg
    52501 tgtataggtt taatttatac acataataat atgtttaagt taggatacaa ggacattcaa
    52561 ctaaaagaaa tttctttaag tggaatagaa atatcagtaa gtaatgtact ttcttgtgtt
    52621 gaggaagcta ttgatatatc taatagtgaa ggtgaattta gagaatattt cttaggaaat
    52681 aacatgattg aatgtataaa aaaaataaaa gatacttctt atagtagcgt agaaggactt
    52741 tctttaaaag tagaaaataa tactatttat gatagagaag acttttttga atttgatata
    52801 gaaagtagtg ttagagaaaa agaattttta aaaacaataa aagtaagagt gaaaattaaa
    52861 aatccatggg taggaattga cattgaagat tatatgaata aatctaataa caataaaatt
    52921 tatgacaaaa aatttaataa ttataaagca tctgaaaata atgaaattct aaatcaattt
    52981 aaaaatgaaa gtgaaaatat aagtaatcaa gaggttgttg aacctacaga agaagacaat
    53041 gaggaatatg aagaagaact taaacaaaac ataattgaaa atataaaaaa gaattttgat
    53101 gaaaaaaaac tagtacaggt atataagtat aggggtgtat aactttgaaa aaagaaggaa
    53161 tgtgactgtg atagaattaa tagtatctat tagtataata ctcttgatag ttacattatc
    53221 ttttccaaaa gataatattg aaaatcataa aataaattta tttgcacgtc aactttgttc
    53281 agatataagg tatgttaggc gaattaatat gtttagtaac tacaatacat atatatacta
    53341 tataaatcaa ggtaaacatg aaggttatgt attgagaaaa gatggtaaaa atattaagtc
    53401 aataatactg cctaaaaatg taaaaatagc tagaagtatt gaaacaataa agtttaaatc
    53461 tgatgggtca cctcaaaagg catctacaat agagatatat aataatagat taagaaaaat
    53521 tataacaata acacctgtaa gtggaagagt acttctgaaa gagggaaaat atgaaacata
    53581 aatatggtta cttacttttg gaaagtgtag tatcattgtc ctcaatgttg ataatgatac
    53641 tagttttata ttctatattt ttgtctacaa taagcttaaa attaaaagta gaagataaga
    53701 tagaactaca acaacaaagt cttgaaatca taaaatctat ggaaggaatt attagcaatt
    53761 ctatgggaat aataaatgta tctaattatg aagacacatt taaaaaggca acatctataa
    53821 aatgtagata tgtagatgaa aatgtaaata ataatgaaga aagcataagc aataaagaaa
    53881 ttatattgaa tgaaagacga aataagttat ttgtaaattc tttaaatggc gaaagtagtc
    53941 aagcaggtgg ttatgaaata ggagattatg tagatgagat gtatgtatca atcaataata
    54001 atggccaata tgttaatatt aaattgaaat tatcaaaacg tagtcaaaaa tatgagactg
    54061 agtttaaaat aaaagtatgg aattttagtg aaaatatata aaaatacata aatttataga
    54121 gaaagaaata atatcaataa gtcatataaa atataattat tgagaactat a
//
LOCUS       DAMJVM010000017        53773 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.017, whole genome shotgun sequence.
ACCESSION   DAMJVM010000017 DAMJVM010000000
VERSION     DAMJVM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 53773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53773
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.017"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            70..414
                     /locus_tag="PC523_001085"
     CDS             70..414
                     /locus_tag="PC523_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2474630.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEILAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            complement(575..2671)
                     /locus_tag="PC523_001086"
     CDS             complement(575..2671)
                     /locus_tag="PC523_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HDF2474631.1"
                     /translation="MIKFKLYISGLYSGNVIFDDDLLIEKLNPFTNKIESLKPISREE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWDKTHKDEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPNFIDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWKPMIHEREDGDSTINWIVSQEWSDGTVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNGMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPIEYYSVGSNIWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDSRLEEKCDSYIYNPKDPSIQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDVFSEEF
                     TITGDIKLEFFASSSARDTDWVIKIMDVDLDGNSIKLADGILSARFRNGFYESEFMEE
                     GKIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(2684..3844)
                     /locus_tag="PC523_001087"
     CDS             complement(2684..3844)
                     /locus_tag="PC523_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HDF2474632.1"
                     /translation="MNTLFSNPGSILAVMTLMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFTSFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            complement(3863..4930)
                     /locus_tag="PC523_001088"
     CDS             complement(3863..4930)
                     /locus_tag="PC523_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HDF2474633.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVISVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNVPLYKVLGGFDSKVQTDITISIDKPEKMAIEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNIKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEESKSISGGF
                     VMDGDIMNLLNKPGLGIEVNL"
     gene            5281..5847
                     /locus_tag="PC523_001089"
     CDS             5281..5847
                     /locus_tag="PC523_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2474634.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTPEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDVCMGEKIVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            complement(6065..7051)
                     /locus_tag="PC523_001090"
     CDS             complement(6065..7051)
                     /locus_tag="PC523_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HDF2474635.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSISGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYISTFIQEKDF
                     TINKKLNSKYIPTYSSEIVLRIVKGPQFDAFSKGEVEKFLSNEYKVTNEIDRMGCRLD
                     GESIKHINGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(7081..7776)
                     /gene="pxpB"
                     /locus_tag="PC523_001091"
     CDS             complement(7081..7776)
                     /gene="pxpB"
                     /locus_tag="PC523_001091"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDF2474636.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLKIDFDSLIESLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEIPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            complement(7796..9016)
                     /locus_tag="PC523_001092"
     CDS             complement(7796..9016)
                     /locus_tag="PC523_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDF2474637.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            complement(9045..9836)
                     /locus_tag="PC523_001093"
     CDS             complement(9045..9836)
                     /locus_tag="PC523_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HDF2474638.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVNKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNAEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELESIEVCPLENIEVCSSENIV"
     gene            10423..11052
                     /locus_tag="PC523_001094"
     CDS             10423..11052
                     /locus_tag="PC523_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2474639.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAKTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(11163..12677)
                     /gene="glpK"
                     /locus_tag="PC523_001095"
     CDS             complement(11163..12677)
                     /gene="glpK"
                     /locus_tag="PC523_001095"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDF2474640.1"
                     /translation="MEKKYIMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDKDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASIQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVNIDRPKIVETTALGAAYLAGLS
                     VGFYIERNEITSKWQVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            12921..13463
                     /locus_tag="PC523_001096"
     CDS             12921..13463
                     /locus_tag="PC523_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2474641.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLDIIFTEIRNFINKVHNVQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            13465..15969
                     /locus_tag="PC523_001097"
     CDS             13465..15969
                     /locus_tag="PC523_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2474642.1"
                     /translation="MVFTLNSNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPA
                     RTIIQNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSL
                     ICGLSSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGI
                     ANILGSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLG
                     TLMLVCIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPIL
                     SFEYFLNPRILIILILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGV
                     AAPLSGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCLSLMFMGLGMGF
                     TMGTPLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMD
                     LVGKPSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPK
                     YLLDKVQSADTTNITNITKEISTTMFDEKVPSIISKIQKNVQKGIDGTQSGINGIEEG
                     ESKLNSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGIDKGIKGMEQGLKKQDKAID
                     ELTTAYNKIPTTEKIPTNNENNSNEQNQDNASNSESDATNNDSTENKNNNSNDNKPNL
                     NEQKESLNAQIQKLKKTRNELNAKLQKNKAQKNELSKKLKSMESQKKELQSKLNNSIN
                     QKKDMEKSLDTMQQQKKSLQAVLEKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENT
                     LQSTLNSGFKQMYITVFCVNLLAFVILLFYKENKTR"
     gene            16387..16785
                     /locus_tag="PC523_001098"
     CDS             16387..16785
                     /locus_tag="PC523_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2474643.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     IVMSIADYFNVTTDWLLYGKEKVNSNNSVKEDKEDYLHINNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(17002..17211)
                     /locus_tag="PC523_001099"
     CDS             complement(17002..17211)
                     /locus_tag="PC523_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474644.1"
                     /translation="MCKLDSDGVIECCKAIDDFITALSNIKSLNMERLNILTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(17243..17452)
                     /locus_tag="PC523_001100"
     CDS             complement(17243..17452)
                     /locus_tag="PC523_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2474645.1"
                     /translation="MDARRKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            17748..18167
                     /locus_tag="PC523_001101"
     CDS             17748..18167
                     /locus_tag="PC523_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2474646.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSISEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKDKNLKKTEYNKTKDEKIDK"
     gene            18395..18850
                     /locus_tag="PC523_001102"
     CDS             18395..18850
                     /locus_tag="PC523_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474647.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(19061..19321)
                     /locus_tag="PC523_001103"
     CDS             complement(19061..19321)
                     /locus_tag="PC523_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474648.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(19584..19937)
                     /locus_tag="PC523_001104"
     CDS             complement(19584..19937)
                     /locus_tag="PC523_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474649.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQNKDISDISIGQVKQDDDITNNFI
                     ANAEIYEMLLSQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(19954..25131)
                     /locus_tag="PC523_001105"
     CDS             complement(19954..25131)
                     /locus_tag="PC523_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome condensation regulator"
                     /protein_id="HDF2474650.1"
                     /translation="MKQNKLLQRGAYFNDKNILIDDFDKRYNDYDFVEFFTGISNSTF
                     GLKSDGNLYACGDNTGFQLGLGKDSSERRMFSKVKIDNVKYVSCGSKHSVAVTKDGFA
                     YGAGTSNVGQLGVIESTVYYEFTKLPIYDVKTVACGYDFTFVLKNDGTLYSAGLNSSG
                     QLGLGDTNNRATFTKVNIDSVKDVITYNQSVFIIKMDGTAHACGLNSNGQLGINSTLN
                     KSVFNKIEGMDNVKQIACGSSHTILIKNDGTMYTTGYNGVGQLGTGNNNNSIVFTLSS
                     INNVKYASCGNNHTMILKYDNTLFSTGQNNYGQLANANKDVASRNTFVKVNVENIKDI
                     KCGSQFNFLINGSKEIFVSGCNLAGQLGSFFHTTFLYEFSKVQSSNLDNYSGLLVNDD
                     YLYVTKDNSEFLNVKLSDNFQDYKKIELTDNNMFIVMNDGTLYACGLNNYGQLGLGDT
                     VNRSVMTKVDIDNVLDIKGNGNSTFVLKNNGTLYSCGLNNYGILGLKDEVNRNIFTKI
                     EIENVKEFCVESEYIIALNHSKEVYGWGYSANSNIERTSNYPDKQNINGIEKIATYYR
                     SVYMINSEGKLYVSGYNSYYQLGTGNSSSTNKTLVSKYRTVTLSTSSSNLSDLPKIVN
                     VFPFNYGCAIIDKSGFVYLAGQHRYPDTSKSNPSINDYSRYGSFIETTSSYYNTYFTQ
                     VNAFNGIEKVIGTSNNILYFKKGSTYVTGYPKTFGSSATGYKSYTTISSEYSNLGSLF
                     IITSSSSKLYGHGVANNGEFGNITNLDGVSSYDTGLKDIKDIIVKNNTVVVVDKNNNI
                     YVTGTNQFNKLGIGEYNNQPIRKFTNITEQSNSFIFMDDIKEITTSRNTMFIVKNDGT
                     AYATGNNSSGQLGLGDIINRNKFTQINLDNIKKISTSIDGNTTFAIRNDGALYSTGLN
                     TKGQLGLGDIVNRNTFTKVNIQNVRDVVLGTTHSHAIKDDNTLYSCGENTHGQLGLGS
                     ESNHPDVLTFTVNNITNVRDVYCSDTTTFIVKDTNIAYCCGYNNNSQLGMGNTTDQYS
                     FIKCMENVKEVIPNEINTYIITIYNTAYSTGLNTDYCLGLNSNSNQSSFSEIPISNVV
                     KVAPNRNNAVLLLTSEGDVYTAGKCSNGSGTGSETPEKIKKIASKAKDIGMNYRCGHY
                     VSDNGDLYGTGFNDCGQLGVGDVTKRDTFIKTNTRVKKILPLEYANIAIKDTNDIYIC
                     GLNNYGQLGVGNRYDSRNNDNRIFNYKHMNFVMGDLTSIKNRHNFILLNNKIVIPTTK
                     DIDYGLVLGNLYKGDLYTELPYENIKEVSISKTHIIILLNDGTMYGCGTNYHGELLQD
                     LSINQVDEFVQINVSDVKHVSCGDNFTYFIKSDDSLWSIGKNSEYQLGIGHNNPVTEL
                     QRITTVSSCKEVHCGKNYTLVVTTGNELFVQGYNDKGALGLGSDNENAIIKFFTKALT
                     DIREIKSYGSDHILVLKNDNSVWVTGKNRDVYKIEQPVEFLKEFTIVPISEDVNTVKD
                     VLATDNTLYIISEVGTTNAAIEITEKSISSIKIKIQDPNKDISRIEMLINGESVKSVS
                     DLTTEKISFEVPPDKIKIGENKILFRAYCKGDDLYASLFIFKESTGNSIIKDSYVMIG
                     NRMYKVVNTTSNEQDITITLDRGLEEDLNLGDPIYQLINKTKVQVKINKSDLFKDMKL
                     VEIKKSDSSYQEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAENNEPQA"
     gene            complement(25153..26139)
                     /locus_tag="PC523_001106"
     CDS             complement(25153..26139)
                     /locus_tag="PC523_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2474651.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVNIGDVNILTTDSKDLSGAINEVVKKIENISFDDVISGQIQTDISVLKNSY
                     NKLSEKVLDILIYLELESEVTVDEAGYWYDTLTNGNNIVAIEGLKLDLNRKCITGEIG
                     NVIFRDVVLPFSANRVRYIHDMDNNFVETKSSNTYLKEQKDITLSKYSYEIR"
     gene            complement(26140..26838)
                     /locus_tag="PC523_001107"
     CDS             complement(26140..26838)
                     /locus_tag="PC523_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDF2474652.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSIVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(26835..27887)
                     /locus_tag="PC523_001108"
     CDS             complement(26835..27887)
                     /locus_tag="PC523_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2474653.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMISGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGENGTVINNGTVI
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKRYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEINISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTAGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(27880..28308)
                     /locus_tag="PC523_001109"
     CDS             complement(27880..28308)
                     /locus_tag="PC523_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2474654.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVSVRSANFNRDILSANVKVSTIYGEVEINV"
     gene            complement(28308..28634)
                     /locus_tag="PC523_001110"
     CDS             complement(28308..28634)
                     /locus_tag="PC523_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HDF2474655.1"
                     /translation="MANPINEFIGIIREEGKHHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMLDRQIETFDTETNQEHKHEVKNPFIDTFESGDMVIMFRIGEKFAIV
                     SKLVSL"
     gene            complement(28650..30179)
                     /locus_tag="PC523_001111"
     CDS             complement(28650..30179)
                     /locus_tag="PC523_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2474656.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLMQSEVSYNFKDKLVEDIAK
                     QVFAENRISVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEGYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRKDFNTARRV
                     L"
     gene            complement(30461..30883)
                     /locus_tag="PC523_001112"
     CDS             complement(30461..30883)
                     /locus_tag="PC523_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474657.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKATVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(30883..33336)
                     /locus_tag="PC523_001113"
     CDS             complement(30883..33336)
                     /locus_tag="PC523_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474658.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASSRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNELNSNIMIILIFKAKSDEIEKTKNKLLGNLKKIGGK
                     VWKIVIKAKDMTKRVISSILGNLKRVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASSIISKVFPKLRLFAGKVWRGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQRDVDNYYEDLLDIADKTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSSKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KVTSKLVSSIKMAQLGGVL"
     gene            complement(33530..33976)
                     /locus_tag="PC523_001114"
     CDS             complement(33530..33976)
                     /locus_tag="PC523_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2474659.1"
                     /translation="MSENGLSKNINIVDLLLNSDTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(34090..34518)
                     /locus_tag="PC523_001115"
     CDS             complement(34090..34518)
                     /locus_tag="PC523_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2474660.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(34532..35596)
                     /locus_tag="PC523_001116"
     CDS             complement(34532..35596)
                     /locus_tag="PC523_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDF2474661.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAILGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(35601..36044)
                     /locus_tag="PC523_001117"
     CDS             complement(35601..36044)
                     /locus_tag="PC523_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474662.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEESCFFVSILS
                     VICTPIMLNTNNKDIVISIKYLPKPQSKSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYALNFSVTLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(36293..36733)
                     /locus_tag="PC523_001118"
     CDS             complement(36293..36733)
                     /locus_tag="PC523_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474663.1"
                     /translation="MENKKDILFKETDERLHNYKYLDIKIKNINLDIKRYENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKIEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIVKLSEIL"
     gene            complement(36837..37034)
                     /locus_tag="PC523_001119"
     CDS             complement(36837..37034)
                     /locus_tag="PC523_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2474664.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            37203..37523
                     /locus_tag="PC523_001120"
     CDS             37203..37523
                     /locus_tag="PC523_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2474665.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFDVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            37605..38111
                     /locus_tag="PC523_001121"
     CDS             37605..38111
                     /locus_tag="PC523_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474666.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGMKIISLL
                     SNLKQNTKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIIGIINSGVTCVYEMADILNVEINFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            38812..39567
                     /locus_tag="PC523_001122"
     CDS             38812..39567
                     /locus_tag="PC523_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2474667.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(39710..40138)
                     /locus_tag="PC523_001123"
     CDS             complement(39710..40138)
                     /locus_tag="PC523_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDF2474668.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(40559..40759)
                     /locus_tag="PC523_001124"
     CDS             complement(40559..40759)
                     /locus_tag="PC523_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2474669.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(40942..42111)
                     /locus_tag="PC523_001125"
     CDS             complement(40942..42111)
                     /locus_tag="PC523_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HDF2474670.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANIEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            42347..43168
                     /locus_tag="PC523_001126"
     CDS             42347..43168
                     /locus_tag="PC523_001126"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HDF2474671.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVHFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDENILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(43244..44635)
                     /locus_tag="PC523_001127"
     CDS             complement(43244..44635)
                     /locus_tag="PC523_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2474672.1"
                     /translation="MLVGKKFNNKKIVTLRTVFVRYSSLFFAITVVLVSILILSFPIL
                     LSLNIILPANYVEKQIYENKDKIISSKQVTKDLIPDLCEYGVYTLNGKVISGTFNKNE
                     SKEVWDLMRGFERRTITSSKNYIKLSRKNEVCIIRYSIVAEFVSPTLRTYLPKPELLG
                     MIIFSIIFLIEIIILSKLFGKKLNAEMELLKNTTEKIEQQDLDFVIESSKIREINNVL
                     FSMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPEL
                     SEYMEYIAKGATQIEKYINTLIEISKTETGYILNKEVINVSEFIEDILIQIEALARTQ
                     NLNVEFSKQGNLPESITIDKELLFRAIMNVISNAIDYSPSQSKLYISVSVSNKYLKFV
                     ITDCGSGFSKADLSKATGQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGILHIENSIK
                     TGGAMVTIEIPIV"
     gene            complement(44769..45539)
                     /locus_tag="PC523_001128"
     CDS             complement(44769..45539)
                     /locus_tag="PC523_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF2474673.1"
                     /translation="MTQLVRIFCSDLIKLKRTFIILMHFCIALIGMGLCLGYYKYSSA
                     DDISKIAAYLQVIAIAFPLLSSIMCSLCIEQEYYSGSYKHMLTSSNPKYLTLISKYII
                     LICLGFGATLVSVLGFKFGVSSISNEVYFTLDFYMISIMILVGSNLFVYILHLFLSLR
                     FGKGASIGVGIVETLLSAVLLTGLGARIWPYIPCVWGVRFISIWSSFSSSKTIEYIKV
                     ESIKGYQSIGLVCGFVTILAFIILCIWFSKWEGKKSEE"
     gene            complement(45541..46260)
                     /locus_tag="PC523_001129"
     CDS             complement(45541..46260)
                     /locus_tag="PC523_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF2474674.1"
                     /translation="MEKLVRAEFQKMKRTFGIRLIWIAPIFSIIITFLLGGGQNGVYN
                     WWYTMFLPGVLTLISSLIVQKDEKKKYRGILSLPIEKKDVWTGKILYCIICCFITSII
                     FMIGIFLVGMLYKEVISVSQNIIGTILLVLLFMWQIPLCMFLASKFGMFITIIINMFF
                     TIIGVAVFSTTEMWYAFPYSITPRLMAPVLHILPNGLPVPEGSEFLNSNVILPGVLIS
                     IVLFILLSLATNAWFNNREVK"
     gene            complement(46262..46969)
                     /locus_tag="PC523_001130"
     CDS             complement(46262..46969)
                     /locus_tag="PC523_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2474675.1"
                     /translation="MSNYILEVKGVSKKFKDEVVVDNISIAIKHNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLKIVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVLGYQGEVSKEQN
                     LEELFMKVVAENRKRGN"
     gene            complement(47089..47625)
                     /locus_tag="PC523_001131"
     CDS             complement(47089..47625)
                     /locus_tag="PC523_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474676.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDDD
                     VREETTSKMINSKNTTKIPIEIISKDGKIVKYLQIDEESTLKDKLRLILDTLSKEYFN
                     GLDMEVEVKEKDNLVKINLIEPDEKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(47727..48797)
                     /locus_tag="PC523_001132"
     CDS             complement(47727..48797)
                     /locus_tag="PC523_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDF2474677.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAELNK
                     ENKLYQEKVNIDKLDVSVDNINVMIRKSSNEDVRVTTRGNNEFYKYSVTLQDKTLVVK
                     GERKSENKIKKIRSFDHLLNTSINSMFSRDYREIRIYVPNNVDINASSISSYLFISDD
                     VDSNNITYKTSSGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSKDEGVFINNIEEYIPENVNIKEKIGRHSNYDNEFNLYSNMPIAKYLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEVIEEFNNDEEYDNDSYDYEHSEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNRLEL"
     gene            complement(48790..49848)
                     /locus_tag="PC523_001133"
     CDS             complement(48790..49848)
                     /locus_tag="PC523_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDF2474678.1"
                     /translation="MSLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIISSLGSPKSIAKELILQTKEKNIQEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMSTDILNGKFSRFKSNIKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSVTIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGIEILAWQLLLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLKDIENKKGEDKYE"
     gene            complement(49835..50167)
                     /locus_tag="PC523_001134"
     CDS             complement(49835..50167)
                     /locus_tag="PC523_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2474679.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DKGGNELE"
     gene            50366..51487
                     /locus_tag="PC523_001135"
     CDS             50366..51487
                     /locus_tag="PC523_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HDF2474680.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKESIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDFSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKEICCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSDSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYRLVS
                     NEFDSIINFMKKNLEEFKTVIHPEKIADYISELIDKHYS"
     gene            complement(51615..53513)
                     /locus_tag="PC523_001136"
     CDS             complement(51615..53513)
                     /locus_tag="PC523_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HDF2474681.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLDDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVSPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEEGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAVENKDYQAASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
BASE COUNT        17653 a         9087 c         6018 g        21015 t
ORIGIN      
        1 agtatagact ttttttaatt tattatttag atttttaata gattttttaa ctatggaggt
       61 atttaaaata tggaaataaa gagatatgaa ggaactggaa gaatgagtag agctgtagtt
      121 cataacaata cagtttattt atgtggacaa actcatgctg aaggaggagt aattgaacaa
      181 acaactgaaa ttttggctaa aatagaagat ttattaaaca aatatggttc agataagcaa
      241 catttactat ctgttactat ttatttaaga gatatgaaag actttgaagc tatgaactct
      301 gtttgggatg cttgggttga aaaaggattt gaacctgcta gagcttgtgt tgaagctaga
      361 cttgctagag agcatttgtt agttgaaatg agcgttgttg cagctacaaa ataatcaatt
      421 tagcttatag ggggggctgt ctcaaaatag agagataagc ctctttttta ttgttgaaat
      481 gatgaaaata tattcgattt catataaaag aaagtgcctc acgaaccaaa aagttcattt
      541 tgagacacct cctcactatt taatctatat aaatttactt attctctatt ggcaatatca
      601 atttagatgg atatttacct ccatgataaa tcgtattctt tgcttctata tattcaatac
      661 tattatatcc ctctgtcgtg ttactatttt ggaaaataaa gtttttagca ctagatgtta
      721 tgtctagtct aattttatga ccaactttaa acgtgtttga tatttttgaa gttataatcg
      781 taaatttata aatttttcct tcttccataa actctgactc ataaaaacca tttctaaatc
      841 tagcactaag tataccatca gctaatttaa ttgaatttcc atctaaatct acgtccataa
      901 tttttataac ccagtcagta tctctggcag aacttgatgc gaaaaattct aactttatat
      961 cacctgttat agtaaattct tcactaaaaa catctgatgt ataacacaac atgtcacttc
     1021 ttttttctaa atctttgtag ttatttggaa ctccaacttc attctctgac atatcaatta
     1081 gttgtattga tgggtctttt ggattatata tataactatc acacttttct tctaacctac
     1141 tatcaaatac taatctacca tttcctaaag atgtgttagc atttccatca cttattaaat
     1201 acatactctt atctatcttg tttattattg gccaattttc ttctgtcttc catatattag
     1261 aacctacaga gtaatactct attggagcag ttgtatctat gttattttct atacctttta
     1321 agtatttatc aaaccataac agataattat aatctaaatc atatcttaat gaactatttc
     1381 ctaaagaaat accatttata tctcttaaag tatttgaatt atgcatccat ggacctagaa
     1441 tagcctttct ctttcctttt tcatagtctt ttataacatc taaggcctct gtagttccca
     1501 taccattatc atcataccac ccagatacaa ccaacgcagg tacatttatt ttatctttct
     1561 gtaaatgcca atccatcaaa tcccaatact catctttttc tactctcttt aaccattctt
     1621 ctagaaattc aattctatac ccaagagctt caacaggtat attctctatt ggtcgtatgt
     1681 ttaaaacatc atcccaatca tctcttacca ttttacttct atcaacttta tttcttgata
     1741 ccataaacgc ccatgcaagc ataccagata cgaaagctcc tccttttctt ggtatgtcaa
     1801 taaaagggct tcctgatgtt actatactaa ctaaagcttt aagatgctta ttacctgaag
     1861 atgcagctgc ccactgaaca tagccaagat atgatgctcc aagcattcca actgttccat
     1921 ctgaccattc ctgtgatact atccagttta tagtactatc tccatcttct ctttcatgaa
     1981 tcataggttt ccacttacct tctgattcgt ttcttcctct aacatcctgt attacaacag
     2041 catatcctct ttgtacatat ttataatata tttctttatc attttctttt ccatatggag
     2101 ttctcataag aattgttggt gcttttttgg tagaatctat aaaattaggg agatatacat
     2161 ctgtagaaag ttttatacca tctttcatat caatcatata atttccacca aatcttatag
     2221 aatatatctc ttcatcttta tgagttttat cccattcttt taatacagta tactcttcat
     2281 atccatccat aattaatata gtacaagttt ctctggataa acaaataaaa cctactattt
     2341 tattatctac aataattata tctagtggga actttttatt tctttgaacc catatatatt
     2401 cattaagttt agaatgcgtt tctcctaagt tatttataac tgtgtcttta tctgtgttta
     2461 ctaaactttt agtatataca tcaaagttat taaataaaga ctttaaatct atttttgtaa
     2521 gatttaaata gtatgtattt tcttctctgc ttattggttt taaactttct attttatttg
     2581 taaaaggatt caacttttca attaataaat cgtcatcaaa gattacattt ccactgtata
     2641 gtccagatat gtataattta aattttatca attaaattcc cccctataat cccagagaca
     2701 tctttattgc atatgccact gatatacctg cataatttcc taatactcca ccaattactc
     2761 ccattacaac tgctacactt acaagtgaat tccattttgc agaagcagct actaaagctg
     2821 aagtaggacc atcagcaatt gctcctacta tagaaagtat tacatattga tattttatct
     2881 ttaaaagtct agttatgcct aaatggaaca caaaagaagt aaaaattatg ctaaaacagt
     2941 aaagtgttat agtaaaagtt gaacctaaaa actcttttat atctactgaa aaacctattg
     3001 tacataaaaa gaataatgca acaaataacc ctaaatctaa atttcccttt agtttcttta
     3061 ctggctttaa ttgagcaatt ataattgaaa ttgttgtaac taaaagtatt cttgctgcac
     3121 tactaaagct tgaattgaaa tatccagcaa gtattgttga gacctctgta acaacaaaac
     3181 ctatagccaa catccaagcg atatctttaa tactccactc ttttgactcc ataaattctt
     3241 cattttgaca atcttctaat tctgattctt ctacatgata tggccaaagt tttttaaact
     3301 tctttgagtt ttttaatatg gctggtagag caaacataaa tatcaatgtt ggtactccaa
     3361 ttacatagtc tgctgcattt gccgcagcaa gagtttgtct acttacatta agacctgttc
     3421 caatggctgt aagattagca cttccaccag tgtatgtacc tacaaacatt ccagcaactt
     3481 tccaaccttc tgatattttt tctgcaaata ctagcccaaa taaaaatgcc atgatactta
     3541 cagttaatac tgctacaaaa attgcaatca aaggttctct tgataacttt gtcatttctt
     3601 ttaaatcaac actcatcaac attatagtca tagaaactgg aatcgcatat gttgaaatag
     3661 ttccatataa ttcagtactt actggaacta cttttaagtt tgaaagtatt attcccatta
     3721 tgattattgt aagtgctggt cctaagcttt taatggcttt gtatctttgt aagtaaaaac
     3781 ccaatgcaat cattaatgtc attacagcaa gtatactacc tggatttgaa aaaagtgtat
     3841 tcatatttaa cctccaattt tatcataagt ttacttctat tcctaaacct ggtttattga
     3901 gaagattcat aatatctcca tccattacaa atcctccaga tatacttttt gattcttcac
     3961 aaaacataaa gctatcaagg tcagcttctg ttatattctt tttagatgct attaaatttg
     4021 ctgctgctgt tataccaatt ctcgtttcta gcatacatcc taacatacaa tttactccac
     4081 ttgcctcagc tatgttgttg atttttatag caggatatat tccacttgac ttcatcaact
     4141 taatattaaa tgtatcaact gcattctttt ttatagcttt aattgcatct attggtgaat
     4201 gcaatgattc atctatcata atctttatat tactcttatt tcttatgtaa gcagtaccat
     4261 ctaaatccca gtgtggaagt gcttgttcaa ttgcatcaac attaaattct tccaattttt
     4321 tgatagtatt tatactgcta tttacgttcc atccttgatt tgcatcaatt ctaattctta
     4381 tactctctcc aacagcttct ctaattaact ttacagcttc aatatcatct tcaggattta
     4441 tacctgcttt aagctttaat atcctaaaac cttcttttac tctttctaaa gcttctattg
     4501 ccattttttc tggcttatct atacttatag taatatctgt ttggacttta ctatcaaaac
     4561 ctccaagaac tttataaaga ggtacattca taatttttcc tttaatatca tataaagcta
     4621 tatcaatagc tgcttttgca gaagtattgc ctataataat tccatccatg attgtatgta
     4681 tggtttcaat atctagtggg tctttaccta ttaactcttt tttaaacata agtaataccg
     4741 aaataacatt gtccaaaact tcacctgtaa ctgctgcaag aggtgctgct tctccaaatc
     4801 cacaatagcc ttcatcagtt tcaattttta aaattatact ttctccatat tcaattactc
     4861 caaatgaaac tactacaggt ttttttaatt taattctcag cttttcaaat ttgatatctg
     4921 ttatcttcat attaccctcc ttatttttga agttataatt caatttttaa aattttatac
     4981 tttaaattat attattactt caatatatat gtcaagataa tttactgaaa atttacgctt
     5041 ttccaaaata taatgtaata atattaattc aattaacact cttccattaa atttttttat
     5101 tattattctc tttatgttaa tttagcaata atttattttc atagactttt ctttgccttt
     5161 aattaaattt taattgtcaa aatctactaa gtattcttat tgccactact tctatgttat
     5221 gctataatta ctttgtttta agcgaatatt ttatattaat tttattattt ggaggttctt
     5281 atgctaggtg aaaagatgag aaatataaga aaaagtaaga aaaaaacatt aagtgatgtt
     5341 tccaaattga cagatttatc tataagttat atctctcaaa tagaaagaga tgctattgaa
     5401 ccatccttat cttcattgag aaaaatcgct gaagttttag acactccttt atatatgttt
     5461 atggatgata acaatactga tgatttagtc attagaaaag aagatagagt tatgatgaaa
     5521 tttccaaaga gtgaaatgtt ttacgagata gtatctccaa tgcctacacc tgagtttaca
     5581 ccttcaattt tatttgtaga gtttgaactt aaaccagaaa gcgaggatgc caaagattat
     5641 atctatcatg catcagaaga aatcatggtg gtaacaagtg gtgtggtgga tgtttgtatg
     5701 ggcgaaaaga tagtgagatt aaatccaggt gactcaacat ttataaaagc taatgtacca
     5761 cataaaatca tcaacccaag taaggataca atagctcgtg gatatggagt tatatcacct
     5821 ccaatttggc caataaaatc taaataaatt caataaacat atctactttt tatagatact
     5881 acaatagatt taaatatcac ttttatttta aaattgaagt atttatttaa atttaacata
     5941 tttaatcaaa ctaaaaaaat agctatttga ctaatctaaa ccatcttaat tatggtaaat
     6001 agtaaatagc tatttgtaag ttatatttta aattataata aaaattatat ttattaaaat
     6061 taaattaaac tgctatatca tttaatactt ttgcttttac aactcttata ttcttcatgc
     6121 tcttgtagat atcttgaatc ttatcttctt gttctctcaa tagtctatgt gcttcatata
     6181 tatccacaag ttcaaatttt actacatcat tcggctttgc ttgtgctaac ttatataaat
     6241 ctacacttat aacgtttcct atcttagtat aaccaccact agtctgtcta tctgataaca
     6301 ttataatagg ttttccatga ccaggtactt gtatagctcc ataagagata ccatcagata
     6361 taatatctgc tccattaatg tgctttatac tctctccatc tagtctacat cccattctat
     6421 ctatttcatt cgtaactttg tattcatttg ataaaaattt ttctacttca cctttagaaa
     6481 aagcatcaaa ttgaggacct tttacaattc ttagtacgat ttctgagcta taagttggaa
     6541 tatattttga atttaatttt ttatttattg taaaatcttt ttcctgaata aaagtactta
     6601 tataatctcc ttttttaagt gctctacctt caaacccacc aattttagct cttgtataag
     6661 ttgacttact ccccattgca agtggaacat ctattccacc agagattgat acgtaggctc
     6721 tagcacctga tttcacacct ctaaatgata atacatcacc tggatttatt tgatatgacc
     6781 tccagttttg aatttctttt ccgtttattt tagcccctaa atctccccca gttactgcta
     6841 tttgcgtata ttcatcaaag gtaattgtag ggcctaacat taaaacttct aaagaagcct
     6901 catcttcaga atttcctact aatatatttc ctactctatg tgcatactca tccattgctc
     6961 cacatccaga tacaccatat tgaccatatc caactctacc tctgtcttgt attaatgttt
     7021 ggaaacctcc taattcaact ttgaatccca tatataccac tccctgtact ataacgatat
     7081 ttaaatactt aagttatttt tttcgatttc tataaattca tctaatgtta ttggaacaaa
     7141 ttttataaag tctccagctc taagcaaaat ctgttcttct ctattttcat catataattt
     7201 taaaggtgtt ctaccaatta tttgccatcc accaggacta tcgataggat atacaccagt
     7261 ctgagaacct gctattccaa cactcccacc ataaattttg gttcttggta tttctaatct
     7321 aggtgtagct attctttcat ccataccacc tagatatgga aatccaggag taaaccctag
     7381 catatatatc aagtattctc tacttgtgtg tatttttata acttcatcta cagtgagatt
     7441 attgtaactt gcaacatttt taatatctat accaaaaacc tcatcatagc atactggaat
     7501 ctcatgtatt ttaggttttg gtaatactat actttccaaa ttatcttcta ttttttttaa
     7561 actctcaatc aaactatcaa aatctatttt tagtggatta taacttatca taagtgacct
     7621 atatgttggc accatttctg ttacaatacc tttaagattt gatatttcta ttgctctcat
     7681 aaatgaaatt acttttttgt ttatatcttc tgatatctca tttccaaatt cagcaaccac
     7741 tgccttatcc cctgatatca agtatctagt ttccataaat gtcacctctt ctaaattaag
     7801 caaataaagc acctaggctg ctaagagaag ttattcctgt atatgcagat attaaaacta
     7861 ctattagtcc aaatattaag agccatgtag gatgcttgta atctccaaca atatcttttc
     7921 tcttagatgc tataagcata attcctaatg tgattggaag tatcaatcca tttaaagccc
     7981 ctgctaaaat taataaagta gctggttttc ctataaatgc cattataagt gttgatattg
     8041 ctataaatgc tattatgaaa tatttttcat atttatctat aaaaggattt agtgtcttta
     8101 agaaagaaac tgatgtatag gcagctccta tgactgatgt aattgcagca gaccataata
     8161 caagcccaaa gaatttatat cctattgctc ctgcactaaa tttaaaagct gaagcagctg
     8221 gattttctgg gtcaagttga agaccttttg aaactactgc tagtattgct aaaaataaaa
     8281 gaactctcat cattgtggca actaatatac ccaaaagaga agatttagtt atttctttta
     8341 tattttcttc tcctgttatt ccaccatcta ttaatctatg tcctccagca aaagttatat
     8401 aacctccaac acttcctcca agtagagtta ttattgggaa tatcaatcct tttggatttt
     8461 ctggttttac cattctagaa actgcctctc caacaggagg atgatttgat attgctacat
     8521 atccaactat aattatcatt cctaatgcta aaaatttagt tatcttatct actagagaat
     8581 ttgaattttt tgacataaat acaaatatag ctattaatcc acttaataca gttcctaatg
     8641 tcatattcat attaaacatt acattcatac caagtgcagc tccaccaaca tttcctatat
     8701 taaaagctaa tcctccaaga gctactaaaa aagctactaa atagcctaat ccaggtatta
     8761 ttttatttgc tatatcttga cctctaagtc ctgatacacc aattactctc catacattta
     8821 cttgagcccc tataaaaagg attgttgtaa ttaaaattac aaatgaaaaa ttcggtccaa
     8881 aatcttgagt aaattgtgca gtctgagtta aaaatcctgg cccaattgct gatgttgcca
     8941 ttataaatgc agcccctata agcgctccaa catctttttt atttttagtt ttatctgtaa
     9001 tattttttgt actcatattt tatctccccc tcatgttatc agtcttatac tatgttctct
     9061 gatgaacaaa cttctatgtt ttctaatgga caaacttcta tactttctaa ttcaaaacgc
     9121 tttcttattt ctttaacaaa ctctattgct tttggattgt ccccatgtac acatatagaa
     9181 tctgctttga tatgtataac ttcacctgtt aaagtttcta cagttccttc ttttaccatc
     9241 ctaacaaccc tatctattgc atgttttgta tcatgtataa ctgctccttc tgcatttctt
     9301 ggaactaaaa aaccattatt atcataagct ctatctgcaa atacttcatt tgcaaaccta
     9361 agtccaattt ctttagcaga atttatcatt tcactattat atagccccaa caatataata
     9421 tctttattga cttcatatat agcttcacaa atccccatag ccaactcttt atctttagct
     9481 gccatattgt ataatgctcc atgtggtttc acatgttgta tattacatcc atttgaactt
     9541 gcaaatgcca ttaaagcacc taattgatat tttgtataat ctttagcttc ctgcttcgtt
     9601 attttcatct cacgtcttcc aaatcctatt aaatctagaa aacctggatg tgctcctatt
     9661 ttcactccat tttttttagc tagctgtact gttttttcca tatgactagg gtcacctgca
     9721 tgaaacccac aagctatatt ggctgatgaa atatatttta aaacttcttc atctaaacca
     9781 atcttatacg ttccaaaact ttctcctaaa tcactattta aatctacttt atacaattta
     9841 tgacccccct tgctaacatt ttgatagaat aagtagtttc aatttttaaa ttttgtttat
     9901 tttatatatt gcatgaaata ttttattact tgtattcaat tttatttttg caactattct
     9961 attgcattta attaatataa ttttaccatt taaggaatat ttttgcaatt attttaatca
    10021 aacattcatt tgtatttctt tttcaaaaaa tgtttttacc attttttagt taacatttaa
    10081 ttattttcac ttaaaacaaa ggctttaacc ttcattttta aagtattttt catttttaat
    10141 cactttatat gcaagacaga atcatttttc ttcattcatt tgtttgtttc atctatataa
    10201 ttatcattct ttgtaaataa aatatcttta tatttttata attaattctt tatatagttt
    10261 taagtataat aaaatcatgt atactaaatg catgttaaaa tattatttta aaacatgcac
    10321 aatatttgta catattgtag acatataaac ctataaatta tacaatagta ttgttaagat
    10381 tatataattt tttaacataa attttgtatt gggggaaaaa caatggataa aaactatacg
    10441 atgccaatat accaaaaaat agcgttggat attgcaaata aaatatacac gggtgaaata
    10501 caagaggatt cagtattatt tggacgctct gttttggctg gtaaatacaa tgtatctcca
    10561 gaaacgataa gaagagctgt caaaatttta gaggacattg gcgttgttaa aagcataaag
    10621 ggtaaaggtg ttattgtact atctccagac aaagcttctt cttttataaa aaaatataga
    10681 gatattacaa atatatcatc atataaatca acactttata atttaataga tactaaatct
    10741 aatttagaaa atgaaatatt agatactata aataaaattt tagactactc taacagactt
    10801 gaaataatca atcctttagt gcccgttcag ttcaccataa attctaattg caaatatatt
    10861 ggtcaaacag ctgcaaaaac taaattttgg caaaatacag gtgctacaat agttgcaatt
    10921 aaaagaggtg aagaacttat aatatctcct ggaccgtata ttgaatttct agaaggagat
    10981 attctcttag ttgttggtga ccaacatatt tacaactcta taccaatgtt tttatatgaa
    11041 aatgaaaagt aaattatata atttagtatg attatataaa aatacaaaat taacaaaaaa
    11101 agagcttcat attttacata aagctctttt taaattcaaa taaatttaac cttgattaat
    11161 ttttaaatat ccaattcatc ttctttttcc cagcttaaag ctcttgatac agcttttttc
    11221 cagcctttat ataatttaca tctcttctct tcactcatat ttggattaaa ctctttttct
    11281 acttgccact ttgaagtaat ttcatttctt tcaatataaa atccaacaga caatcctgct
    11341 aaataagcag ctcctaaagc tgtagtttct actatttttg gtctgtctat atttacattt
    11401 aatatatctg actgaaattg cattaagaaa ttattattac ttgctccacc atcaactttt
    11461 aatgacttta attttaattt tgaatcattt tgcattgctt ctaaaacatc ctttgtctga
    11521 taagcaattg actccaatgt agctcttact atatgttctt tttttgctcc tcttgtaaga
    11581 cctaaaattg aacctctagc atacatatcc caataaggag ctccaaggcc tgtaaatgct
    11641 ggaactacat atacaccatt agtatcctct actctatttg catacatttc actatctttt
    11701 gcagattcga ttatctttaa ttcatctcta agccattgaa ttgatgctcc tccaataaat
    11761 atacttcctt ctaatgcgta tgttactttt ccatcaaccc cccaagcaat tgttgtaagt
    11821 aacccatgct tagatttaac tatgttactg cctgtattca taagtaagaa acaacctgtt
    11881 ccataagtat tttttgcagt accttcttca acacaatttt gaccaaacaa tgctgcttgt
    11941 tggtctccag cacatccagc aataggtatt ttagctcctg ataacatgcg ctcatctgta
    12001 tgaccataaa cataactaga tggttttact aatggtaaca tgcttattgg tatatccaat
    12061 tctcgtaata tatccttatc ccattctaaa tcatttatat tgtacatcat tgttctagat
    12121 gcatttgagt aatctgttac atgtactttc ccacgagtta agttccatat taaccatgta
    12181 tctatagttc caaataataa ctctccttgt tcagcttttt ccctagctcc ttctacatta
    12241 tctagtatcc attttacttt tgttgctgaa aagtatgcat ctatcaaaag acctgttttt
    12301 tcttttattg attcttccaa tcctttattt ttaagttcat cacatatttc tgatgttctc
    12361 ctacactgcc atactattgc attataaatt ggtttcccag tatacctact ccaaacaact
    12421 gtagtctctc tttggttagt tattcctatt gaagcaattt cttctggtct tatacctgct
    12481 gtttctataa cttctctcat tactccactt tgtgaacccc atatttccat agggtcatgc
    12541 tctacccatc cagcttttgg ataaatttga ttgaactctt tttgtgcaat ttttactatt
    12601 tcgccttttt tattaaatag tatagctctt gaactggttg ttccttggtc taatgccatt
    12661 atatacttct tttccatcat catattctcc ttatcatata aatatttaat ttattaaaaa
    12721 gaaaatttaa cattgtatat ttcaataaat aatcatactt aagtacaaat ttaatacaat
    12781 agataattta taagaagaca cttattttct atcatatcta cataataata ttatacatta
    12841 tagcttacat attgattaat aaattttaaa ggagtataat aaacagtgtt cataatattt
    12901 ttaatgaaag aggtggtaat atgccaaaga ttttagaaaa tgtaaaagaa gatatattaa
    12961 aagtatctcg agatatgatt ttagaagaag attactcgaa tattagcata aggaaaatag
    13021 ctcagaggtg tggtataagc gcaggtacag tgtataatta ctttaactca aaacaagaaa
    13081 taatagaata cattacaaaa tctgaatggg atttattaat aaggcgaatt gaatactcaa
    13141 ataaaaatac tgaagattac atcaaaaaac tagacattat ttttactgaa ataagaaatt
    13201 ttataaacaa agttcataat gtacaataca atgacttttt aaatactttt gaaacagaga
    13261 gattttttga aatgaaaaaa cacaaggatg actttcatga acagctttca aataaagtat
    13321 atgaagcttt agaaaaagaa agctttttta ataacgataa attagtatgc aatatcataa
    13381 taaaaatgtt tttttcatat tcaacatatc ttcatataga atttgaagat ttaaaaccct
    13441 atattgaaaa attaatacat taaggtggtg tttaccttga attctaatac tagtattaag
    13501 aacaaggaca gtaatccaaa aatattttta gtacttacac tttatctatt aggtatattt
    13561 atgggagcta ttgatactgg aatagtatca ccagcaagaa caattataca aaatagcctt
    13621 ggagtaaatg aaaaaacagg aatttggatg attaccatat acacattaac atatgcttct
    13681 gtaattccta tctctggaaa attagcagat aaaataggaa gaaaatatgt gtatctagtt
    13741 agtatatttc tatttggtag tggttcatta atatgtggat tatcatcact attttccaac
    13801 ttctatattt tactaatcgg tcgtgttatt caagcaattg gtggtggtgg aattatgccg
    13861 attgctacag cagaatttgg aactaccttc ccagaaaata aaagaggtat ggctttaggt
    13921 ttagttggag caacatatgg tattgccaat attttaggtt caagtattgg tagcacaatt
    13981 ctaagcatat ttggaactca aaattggaag tggctatttt ttgtaaatct cccaatctgt
    14041 ttaatcatac ttattggtgg agttttctgt attaaaaaca ataaatctga atctaaagaa
    14101 aaaatagata agcttggtac attaatgttg gtatgtataa tcgtatcact attatatggt
    14161 cttatgaata tagacttttt taattttaaa aattctatac aagatatatc agtttatcca
    14221 tacttattga catttattgt acttatacct attttcatat ttattgaaaa tagagctaaa
    14281 gaccctattt taagctttga atatttttta aatccaagaa tattgataat acttatttta
    14341 tctttaattg ttggtattgg aatgatgggt atggtattcg ttccacaata tgctgaaaat
    14401 gcattaaaga taaatgctgg ttctggtgga tattttgtag ctatactagg attatttgca
    14461 ggtgttgctg ctcctttaag cggaagactt attgataagt ttggagcaaa gaaaatacta
    14521 ttactaggat tttctgtgtc tatgataggt tctctttatc tagtcttaat tgctctaaaa
    14581 accaatacag tattcagtgt atgtctaagt ctaatgttta tgggattagg tatgggattt
    14641 actatgggaa cacctctaaa ctacatgatg ttatcaaata caagattaga agaatctaat
    14701 tcagcacttg caacattatc cttgatacgc tcaattggta cttcaatttc tcccgctata
    14761 atgattggat ttatagcaca tgcaggatta tctgtacaag ataatattat ggacttagtt
    14821 ggaaaaccct caacgcctaa aatagttcaa cttgaagaac ttgaaaacat gatagatgac
    14881 ttaaaatcag accctgaaat ggcaaaaaat ttaaaaaatg tctctattcc aaatatgaat
    14941 gatacttcaa atataaatat ggacatgaca ggtggcaaac ttccaaaata cttacttgat
    15001 aaagttcaat cagcagatac aaccaacatt acaaatataa caaaagaaat atcaacaaca
    15061 atgtttgatg aaaaagttcc atctataatt tctaaaatac agaaaaatgt acaaaaagga
    15121 atagatggaa cacaaagtgg aataaacggt attgaagaag gtgaaagtaa actaaatagt
    15181 ggtattaaag gagttcaaac tggtatcgac aacatggtaa aagctagagc tggtatcaat
    15241 caaggaatag atggtgtaaa aaaaggtatt gctggtattg acaaaggtat aaaagggatg
    15301 gaacaaggtc ttaaaaagca agataaagct attgatgaac ttacaactgc ttacaataaa
    15361 atacctacta cagaaaaaat tcctactaat aatgaaaata actcaaatga acaaaaccaa
    15421 gataatgcca gtaattctga atcagacgca actaataatg attccacaga aaataaaaat
    15481 aacaattcca atgacaataa accaaattta aatgaacaaa aagaaagttt aaatgctcaa
    15541 atacagaaat taaaaaagac aagaaatgaa ttaaatgcaa agttacaaaa aaataaagct
    15601 caaaagaatg aattaagtaa aaaactaaaa tcaatggaat ctcagaagaa agaactacaa
    15661 agtaagttaa ataattctat aaaccaaaag aaagacatgg aaaaaagcct tgatacaatg
    15721 caacagcaaa aaaaatcact acaagcagtt ttagaaaaaa cacaggaggt aaaaactgaa
    15781 ataccaaagg cttttgataa atcaaaattg gattatataa attccataga aaataataga
    15841 acaaaaatag agaatacact acaatctact ctaaactcag gatttaaaca gatgtatata
    15901 acagtattct gtgtaaatct actagcattc gtaatattat tgttctataa agaaaataaa
    15961 actagataaa agagtgttat agtattatat tgtaaatcta tcttattctt aaatatttta
    16021 tcctaaagaa taaatgcata aaaatttttg taatggatta atataaatta caatataata
    16081 ctatttttct attaatcttt tttactatta aaggaatcaa aaggtatctt agcttatcta
    16141 aataaattta tacaatctat tcatacacac ctttacattt ttataacaat taaacttatt
    16201 gttatataca aatcaaaata aaccatatat tttcaactat aatatgttaa aatcatatta
    16261 tgagcatatt aatttaagca aaatgtcatt tagactttaa tttagtagtt ttatattaca
    16321 attttttata tttttaattt taaatgtcat ttagacatta tttataaaaa gaaaggagtg
    16381 tattttgtgg atactttagg aaaaagaatt gcttatttaa gaaactccaa aaaacttact
    16441 cagcgcaaac ttatggatat tttaaaattt gaaaacttag gcaagtatga aacaggagat
    16501 agaaaaccta attgcgatat agtaatgtca atagctgatt attttaatgt tacgactgac
    16561 tggcttctct atggtaaaga aaaagtaaat tccaataata gtgtaaaaga agataaagaa
    16621 gattacttac atattaataa tgatgaaatg actatattaa atctatatag acaattaaac
    16681 gaacgtgata aaataaaaat agaaggaata cttgaactta agatatctga atacaaagac
    16741 ttaaaaaagc attcctctaa taataatgaa gataaaatgg tttaaacata cataatttga
    16801 tacgatttta catcatctat taactttctt ttttggtagt atattactaa aatatggata
    16861 ttggattatt taaaaacaag tatagtctaa tttcaagcac atttataata tttagattat
    16921 cttatccata ttttagttta cgtactttga aaaatataat acatttctat gtataattta
    16981 tattcctaag aaatgtattg ctcatgtttt taattcttcc aacatctttc tatatacaat
    17041 tgtacatcct tcataattcc cctctttaag aaggattgaa catgtactag aatatttagt
    17101 caaaatattt aacctttcca tatttaagct ttttatatta ctaagtgctg taataaaatc
    17161 atcaattgct ttacaacact ctataactcc atctgaatcc aatttacaca caagaatatt
    17221 cacctacttt atatactatt tattaatcat cttctgaata ttctatatta agcaattgat
    17281 aaattgatga tttatatttt ttacctgttc ttctcccatt taaaatagct gacaaatagt
    17341 tttcattaac acctattttc ttcgctaatt ctctttgagt catatttaat tcaataagcc
    17401 tttgcttgac ttgtactccc aaaaaaggta tccattttct tcgagcatcc atttatcaac
    17461 ttcctttcta agtaatttat atttactttt atattttagt ttacgcaccc cctctattta
    17521 gtatatattt ttaatatgta cacatattaa gtatataaat ttatttaata ctaaataaga
    17581 ttttttgatt actttgtgct aaaattagga tgtaaattcg tatcattaag ttaattataa
    17641 agtcattttg accttaagtc aatgattttt atgtcatttt gacctttatt ttaaaactat
    17701 ttatgtctta aaaagtcttt ttgacattaa ttgaatggag agtgtttatg gatactttag
    17761 gagaaagaat tgtttatttg agaaaagcaa aaaaccttaa acaatatgaa ctagaagaaa
    17821 tgttgggctg tgacaacttg agtaaatttg agagaaacat tagaaaacca aattatgaaa
    17881 tattaaaatc tatatctgag atatttaatg tttcagtaga ctggctatta aatggagata
    17941 atctatcaca taaatcagat ttaatttgcg attcctcctc aaactatcca cttaatagca
    18001 taaactctaa tgaaataaaa cttttaaaca actttagaaa attaaatgat tatgacaaag
    18061 ccaaaataga aggtatgatt gaattaaaac ttcatgaata tgaaaaagat aagaatttaa
    18121 aaaaaactga gtataataaa actaaagatg agaaaataga taaataatat ttatgttgct
    18181 tttgatacga ttttacatca tttctttgtt ctaatatttt tatttcattt attttctttt
    18241 ttataatttt atgataaaat aattagtata ttttgcttta cttgaatgta attatttata
    18301 caaaagtcaa acataatata aatgtagtaa atttttagtt tcaagtaata tctcgttatt
    18361 ataactaaat atttattaaa attaggagga taaaatgggc ttaagagaca aattcgcgca
    18421 atcatttgct agatcaaaaa ctatgagtgg ccctgagaaa aaggctaatg aaataatggg
    18481 taaattgctt cttaagaaag ctatattacc tatcgtttta atgttcgtga taataatcgc
    18541 tggagctatg cttaaaatca attcttgggt tacacttggc ataaatttag ttattgctgt
    18601 tggtgctttc ttctacataa gaaattctag taaaaagtat cagaacttta aaccttatgt
    18661 tggcaatttg attagtttag agaaaaaagg taaaaaagag tacgttgcta ttataaaaca
    18721 aggtaagtta ccagttaagt tacaaattgc ttatggtggt gaagaccttg aacatgttaa
    18781 gaaaaatcaa atggttcaga taagttataa cccagatgct aaaatagcta ttttggttaa
    18841 tagacaataa tttaataaaa aaaggagttg tcttaaaatt gattttttct aatcaaattt
    18901 aagctcaact cctttttgaa tttttatata ttatttttat tacttttttt aattaatatt
    18961 ttaaaatatt aaatccttct caataaaata gggtactatc tacaatacta ctttatcatt
    19021 tcccaacaat atataatctg gtgctactgc tacgttcatg ttatacaccg ttttgtaaca
    19081 taccttttat atcagtaaca tcttccttaa tttcctctac atctgttttc attattccca
    19141 tttgaacaag tatattctta tttatttctt gttgttgtgt agataattct ataaaatttt
    19201 ctacagtttt tttatacatg tctctatctt cttttttctc ttgcatagta tttttaaata
    19261 atagagcaca tagtatgccg attgccccta gactactcaa ttcggttatt aatttatcca
    19321 ttatagcctc ctattgtcca atttttttat aattataact ttaatttttg ttacattctt
    19381 acatcatgca atattatatt ttcaggatta tcaatttaat cttttgtatt ttaaattgta
    19441 ggtgtaattt atagattgaa taaataataa atatatatac aatttctaat ataacaaaaa
    19501 ataagtacaa ggcttactta ttaaagttaa gaccttgtac tttacataaa aaaaataact
    19561 aatacatagt tattctaaat ttactattca actggtattt ctaattcttt acatctaatt
    19621 ctaacttgct ctctaattac tgctggtact tcttctattg tttttttgcc tcttaaaatt
    19681 aaagctacat atacttctac cattccactc tcacctccag attttcttac actaaaagtg
    19741 cttatattac ttgcttcatt gacagaactt tgacttaaca acatctcata tatttccgca
    19801 tttgctataa aattatttgt tatatcatca tcttgcttta cttgacctat acttatgtct
    19861 gagatatctt tattctgcat ttctactttt ctaccttgtt tgatttgttc tattttattt
    19921 ttatcgaatt taaacaattt tcacacctcc attttaagct tgtggctcgt tgttttcagc
    19981 atcatatcta aaaatcatag atggacgttt tatagctgtc catttatctc ctttttctac
    20041 tatgatttta ggctgagcac tttttatgtt ggcttcttct aattcataga tttcttggta
    20101 acttgagtct gattttttga tttcaactag tttcatgtct ttgaataagt cagatttatt
    20161 tatttttact tgaactttag ttttatttat taattgatat atagggtctc caagatttaa
    20221 atcttcttca agccctctat ctagtgtaat tgtaatatct tgttcattag atgttgtatt
    20281 aactaccttg tacattctat tacctatcat aacataagaa tctttaatta tagaatttcc
    20341 agtactctct ttaaaaataa ataaagatgc atataaatca tcacctttac aataagctct
    20401 aaatagtatc ttattctctc ctattttaat tttatctggt ggtacttcaa atgatatttt
    20461 ttcagtagtt aaatcactta cagattttac actttcacca tttataagca tttctattct
    20521 acttatatct ttattagggt cttgtatttt tatcttaatt gatgaaattg atttttcagt
    20581 aatttctata gcagcatttg tcgttcctac ttctgatata atatataatg tattgtctgt
    20641 tgcaagtaca tcctttactg tatttacatc ttcagaaata ggtactatag taaattcttt
    20701 taaaaattct actggttgtt caattttata tacatcccta ttttttccag taacccatac
    20761 tgaattatca tttttaagca ccaatatatg gtcacttcca taagatttta tttctcttat
    20821 gtctgttaat gcttttgtaa agaattttat aatagcattt tcattatcgc ttcctaaccc
    20881 taaagctccc ttatcattat atccttgtac aaataattca ttacctgtag ttactactaa
    20941 tgtatagttt ttaccacaat gtacttcttt acaactagat acagttgtaa ttctttgtaa
    21001 ttcagtaact ggattattgt gacctatacc taattggtat tcagagtttt taccaataga
    21061 ccaaagacta tcatcagatt ttataaagta agtaaagtta tctccacatg aaacatgctt
    21121 tacatctgat acattaatct gcacaaattc atctacttga tttatagata aatcttgcaa
    21181 taattctcca tggtagtttg taccacatcc atacattgtt ccatcattaa gtaatataat
    21241 aatatgagtc ttagaaatag atacttcttt tatattttca tatggaagtt cagtataaag
    21301 gtctcctttg tacaaatttc ctaatactaa accataatct atgtcttttg tggtaggtat
    21361 aactatctta ttatttagaa gtataaagtt atgtctgttt ttaatagatg tcaaatctcc
    21421 cattacaaag ttcatatgct tataattaaa tattctatta tcattatttc tactatcgta
    21481 tctatttcca acacctaatt gtccatagtt atttaatcca caaatatata tatcattagt
    21541 atcttttatt gctatatttg catattctaa aggaagtatt ttctttactc ttgtattggt
    21601 ttttataaat gtatctcttt ttgttacatc accaacacct aattgcccac aatcattaaa
    21661 acctgtacca tataagtctc cattatcgct tacataatgt ccacatctat aattcattcc
    21721 aatgtccttt gcctttgatg ctattttttt aatcttctct ggagtctcac ttcctgtacc
    21781 tgaaccatta ctacatttac ctgcagtata tacatcccct tcacttgtaa gtaaaagtac
    21841 tgcattattt ctgtttggag ctacttttac tacatttgaa attggaattt cagaaaatga
    21901 actttgatta ctgttactat ttagacctaa acaataatca gtatttaaac ctgtactata
    21961 tgcagtatta tagattgtta ttatataagt atttatttca tttggtataa cttcttttac
    22021 attttccata cactttataa aactgtactg gtcagtagta tttcccatac ctaattgtga
    22081 attattattg tatccacaac aatatgcaat gtttgtatcc tttacaatga atgttgttgt
    22141 atctgagcag tacacatctc ttacattagt tatattgttt acagtaaatg tcaatacgtc
    22201 tggatggttg ctttcgcttc ctaagcccag ttgcccatga gtgttttctc cacatgaata
    22261 taatgtgtta tcatctttga ttgcatgtga gtgggtagtc cctaaaacaa catctcttac
    22321 attttgtata tttactttgg taaatgtgtt tctatttact atatcaccta atcccagttg
    22381 tcctttggta tttaatcctg tggagtatag tgctccatca tttctaattg caaatgttgt
    22441 gttaccatct atacttgttg atattttctt tatattatca aggtttatct gagtgaactt
    22501 atttctattt attatgtcac ctaatcccag ttgtccagaa ctattatttc ctgtggcata
    22561 ggctgttcca tcatttttta ctataaacat tgtatttctt gatgttgtaa tttcttttat
    22621 atcatccata aatataaatg agtttgattg ttcagttata tttgtgaatt ttcttattgg
    22681 ttgattgtta tattctccta tacctagttt gttaaattga tttgttcctg ttacatatat
    22741 attgttgttt ttatctacta ctactacagt gttatttttt acaattatat cttttatgtc
    22801 ttttaatcct gtatcataac tacttacacc atctagattt gttatatttc caaattctcc
    22861 attattagca actccatgtc catatagttt agaactgcta gaagttataa taaaaagact
    22921 tcccaaattt gaatattcag aactaatagt agtgtaactt ttatatccag tagcagaaga
    22981 accaaatgtt tttggatatc cagtaacata tgtactacct tttttaaagt ataatatatt
    23041 atttgatgtt cctattactt tttctattcc attaaaagcg tttacttgtg taaagtaagt
    23101 attataataa gaactcgtag tttcaataaa acttccatat cttgaatagt catttatact
    23161 tggattgctc tttgatgtat ctggatatct atgctgtcct gctaaataaa caaatccact
    23221 tttgtcaatt attgcacaac cataattaaa aggaaaaaca ttaactattt taggtaaatc
    23281 acttagattg ctagaactag tagacaatgt aactgttcta tacttagata ctaatgtttt
    23341 atttgtgcta ctactattgc ctgtacctaa ttgatagtaa ctattatatc cagatacata
    23401 taattttcct tcactattta tcatatatac agaacgataa tatgttgcta tcttttctat
    23461 accatttata ttttgcttat ctgggtaatt agaagttctt tctatattac tattagcact
    23521 ataaccccac ccatatactt cttttgaatg atttagtgct ataatatatt cactttctac
    23581 acaaaactct tttacattct ctatttctat ttttgtaaat atatttctat taacttcatc
    23641 ttttaaccct aatatcccat aattatttaa accacatgaa tataatgttc cattattctt
    23701 aagtacaaaa gttgagtttc catttccctt tatatccaaa acattatcta tatccacctt
    23761 agtcataact gacctgttaa cagtatctcc cagtcccaac tgtccataat tatttaaacc
    23821 acaagcatat aacgtaccat cattcataac aataaacata ttattatctg ttaactctat
    23881 cttcttataa tcctgaaaat tatcacttaa ctttacattt aaaaattcac tattatcctt
    23941 tgtaacatac aaataatcat cattaaccaa taaacctgaa taattatcta aatttgaaga
    24001 ttgtaccttt gaaaactcat acagaaaagt tgtatgaaaa aatgaaccaa gttgacctgc
    24061 cagattacag ccagatacaa atatttcttt tgaaccattt attaaaaaat taaattgaga
    24121 accacattta atatctttta tattttctac attaacctta acaaaagtat ttctggatgc
    24181 tacatcttta ttggcattgg ctagttgacc ataattgttt tgtcctgtac taaatagtgt
    24241 attatcgtat tttaatatca tagtatgatt atttccacaa gaagcatact taacattatt
    24301 tatactagaa agagtaaata caattgaatt attattattt cctgtaccaa gctgaccaac
    24361 tccattatag cctgtagtat acatagttcc atcattctta ataagaattg tatgactact
    24421 accacacgct atctgtttta cattgtccat accttctatt ttattaaaaa cacttttatt
    24481 taaagtacta ttaattccca actgcccatt tgaatttaat ccacatgcat gtgctgtccc
    24541 atccattttt atgataaata cagattggtt ataagttatt acatccttta cactatctat
    24601 atttacttta gtgaaagtag ctctattgtt agtatcacct aatccaagtt gaccacttga
    24661 gtttaaacct gctgaatata atgttccatc atttttaagt acaaaagtaa agtcataacc
    24721 acatgcaaca gtttttacat catatattgg tagcttagta aattcataat atactgtaga
    24781 ctcaattaca cctaattgac ctacattgct tgttcctgct ccatatgcaa atccatcttt
    24841 agttactgct acactgtgtt ttgaaccaca agatacatat tttacattat caatttttac
    24901 tttactaaac atccttctct ctgacgaatc ttttccaagt cctagttgaa aacctgtatt
    24961 atcgccacaa gcatataaat taccatctga ttttaaacca aaagtactat tacttatacc
    25021 agtaaaaaat tccacaaaat cataatcatt atatctttta tcaaaatcat caatcaatat
    25081 gttcttatca ttaaaataag caccacgttg taaaagttta ttttgcttca ttattagtac
    25141 tacctccttt atttatctta tttcatatga atatttactt agagttatat ctttttgttc
    25201 ttttaaataa gtattactag attttgtctc aacaaagtta ttatccatat catgtatata
    25261 tctaactcta tttgcactaa atggtaatac tacatctcta aaaatcacat taccaatttc
    25321 acctgttatg cattttctat ttaaatctaa cttaagccct tctatagcta ctatgttatt
    25381 tccatttgtt aatgtatcat accaataacc agcctcatct acagttacct ctgattctaa
    25441 ttctaggtat attaatatat ctaacacttt ttcagataat ttgttatagc tattttttaa
    25501 tactgatata tcagtttgta tttgaccact tataacatca tcaaaagata tgttttctat
    25561 ttttttaact acctcattta tggctcctga taaatctttg gaatctgtag ttaatatatt
    25621 tacatcacca atgtttactt ttatttcttt tatattttta tcgaacttat catctaaatc
    25681 ttgaatatcc ttttgagtta caaaaaccaa tgtcgggtct acttctaatg taacatttga
    25741 agtattggac aattgtaaaa tcatttttac aaccaaatct ttaatactgc catcttcagc
    25801 acgtggctta tacgtctctg gatacttacc tatagctatt atattattct cattatccag
    25861 aacagcagct tctcttatca taaatccacc aactgttcct ggtaaaatag tctctataac
    25921 tatccaatta ggattttttt catcaattct tagagaattt atatttcctt cccaaactac
    25981 attctttaaa gcagtttgtt cctctgttgg ctcataatat tctccattac tatctccaac
    26041 ttgaaacttg gttagtccaa caagttctcc tagtgcagtt gcatttgcta ttttttctct
    26101 accaattttg gttaatatta tataaaattt ttcttccaat tataccacct ccgatttagg
    26161 ataaactact acattttcta cacctgtatc atttgcaata gctatattaa tcttacaatt
    26221 attttcaata catttaatgc ttaaaggata aacctttatt tcttctctac ttaacattcc
    26281 agctccacag tttacaacag cactgcattt tataattggt tcaaagtatg gatgaacttt
    26341 tacctcttca ctacatacca tacctccacc acaatagact atacttttat ctagtattat
    26401 tggttctaaa tagtgtaata agtgagcagg ttttactctt tcaatcatat cgctacaatc
    26461 taaaagagtt ttatagtcac agttatttgc tataaaactc aatacgaatg taaattcatc
    26521 actataaact tttatatctg tttctgattt tgtatatgcc tcacatatac tttttataac
    26581 ttcaatagta ctagtacctc tgcttctcat tttagctttt atattgctac gtcttgtgtc
    26641 aaaatcaagt tctttttttt caatgcagag tatatcttcc cacatatcta atccccatgt
    26701 cgctgtatca acaaataatt ggttgaaggt atcatcaata tcttctttaa gaatatttaa
    26761 ttctgtatcg tatgcacctt gtatctcttc tacaatgtaa tttctatcaa atgatggtag
    26821 tttatcaatc agcttcattg attttccacc tcactaaaat taacagttgt tacactaggt
    26881 attttatctt cattaatagt tatattgtct gtacttccat ttataagtag gttgcttaaa
    26941 tcatgtacac cagcagtatt tataagtatt cccatgactt ttgtatagat tatctctcct
    27001 ctaatatctc taaagtatgt atttatactt tctaggaaag attctttgac attatccaat
    27061 gtatatccat cttctagttt cattactgca cttatattta tttctattgg taatggcgtc
    27121 acaactgtta tagttggtcc aataggcttc tcttcatcta tatgttgttg acatctttca
    27181 atcgtttctg tatcaacagc ttgattattt ttcccaaata tcaagacttt aactgttcct
    27241 ggaccatccc atcttggata aaccttaaca ttatagactc catctacttc caaagcccat
    27301 tcttcataat gagctttatt tccacttgta gcttgatttc tttggatttt ataaaatctt
    27361 tcctttagct cttcatctgt ctctatatct gtaccacctt caaaacttct tgtgttagta
    27421 atctttgtta ctccagatat attatcaact agtttaaatt cacaatttgt aggtaaatta
    27481 tatctcttac caacttccag agcttgaact ggactattgt caccttcctc actaccaata
    27541 gttacatctt ttattactac aaagagtaaa tctctatatg atattactgt gccattattt
    27601 attacagttc cattctctcc aataaattca acttctccat ttgactctgt acccaacttt
    27661 ctatatacac caaattcatt gactctttta tcaagaaact ggttatatgt gtcttgtata
    27721 aaagccattt tatgtatctt tgaaagttct agatatatct ttgaaagttc tagattgttt
    27781 ccagatatca tgttgtttag aaaagaacct tctcctttat aaatatcaag attaatattt
    27841 tcaagagttc tattttttat tacttcatat gtctggtcac tatacattta tttccacctc
    27901 cccatagata gtggatactt ttacatttgc actcaatatg tctctgttaa agttagcact
    27961 tcttacactt acttctaata tatatggatt tattagaagg gcctctttta tgaatctact
    28021 agcttcactt tctgtaagac cttttgtata tttttgtcct attagttctg aaagctctgt
    28081 cccatattct aaagagtata tctcatgttc atatctattt gtctttatac acttgtatat
    28141 ccaaactttt aaggcttctt ttttctcaat tattttaaaa tcaccttttt ctaaaatagg
    28201 ttcatctttt tcaaaatccc atgccacttc acgaaagatt ggcaattctt ctgttttagg
    28261 taagatataa tcctctggga cacctataaa aggaaatatt gtactcatta taagctcacc
    28321 aacttactta caatagcaaa tttttcgcct attctaaaca ttattaccat atccccagat
    28381 tcaaaagtat ctataaatgg attttttact tcatgtttgt gttcttgatt tgtttctgta
    28441 tcaaatgttt ctatctgtct atcaagcatc caactatcta tcaaaatatc ttctttttct
    28501 aatatgatgt tatttgtctc tatctttaaa tctggtaatt tacttttaat ttttccaata
    28561 aaaaaagaag gttgattatg atgctttcct tcttctctta ttattcctat aaattcattt
    28621 attggattag ccattatatc accatccttt tataatactc tccttgctgt attaaaatct
    28681 tttctactac ttaacttact tattttaact acatcacctt tttgtggagc atgtaaaaat
    28741 tctccattac ctatatatag tccaacatgg ctgactggat tatgaaaaaa tactaaatct
    28801 cctgcttgaa gatttttttg ttctactttc ttgccttttt tagattgttg atttgatgtt
    28861 cttggcaaac taacattaac ttttttaaaa caatagacca ttaaaccaga acagtcaaaa
    28921 ctacttggtc catttccacc ccatttatat ggtttaccta gatgtttttt tgcttctgat
    28981 attactattt tagctttatc agatacatta ccagaataac caccgataat aatttttcct
    29041 tttcttcttc caaatttact agcttcttca acatttccaa ataaaatatc tatgtggtat
    29101 gttccattct tttctatagt tattgcaggt cctacgtcat ttactttata gactccatca
    29161 cgaccagaaa ctcctgtaat tacctgtatt tcatctccat agtttaatag aggatgttta
    29221 tttaaaaact cttttgtata ataagtttgc tcatatttac caaccatagg agcagcgcaa
    29281 gtttttttag atgggtcaag ttttttcttt ctacaatctg tatctccacc ttcttctttt
    29341 ctaggacagt aagctgtaaa ttctgctgta aactcttttc ctcctgcata accttctcct
    29401 cctaaattac tttcttcctt aggttcatcc tgtcctgctg acttttcatc cataagattt
    29461 tgaaaattaa gttcaagttc aatttgatac tctccatttt gccaggtatg tttgtctgta
    29521 tctatataaa atagtcctac aagctttgta taagaatctt tgacttttac ccctctacca
    29581 gttatacaac ttacatctcc ataaccttta agagaacagc ttttttctat cccattaaac
    29641 tcgctatcaa tatctacatc ttgattttct tgttgctgaa ttactttttg cattattaca
    29701 tttacttcct taaaaatttc attatctatt ttttcgctaa tcttacttcc atactggtct
    29761 accactatta ctttattttt tacattttcc atactctccg aaaaggtggt atttataata
    29821 ttaaatccct cttcaaacat aacacttaat gtaacagttc ctttttcaat aacattaaac
    29881 ttatctaaat ttgcctctat catatacttt ttctttgtct ttttacttgc ctctgtatat
    29941 gcactcatta ttgtgtcata accatttact cctataaaca tctttgtata cttgacattg
    30001 gtctttgcta ttgttccaac tgaaatccta ttttcagcaa atacttgctt tgctatgtct
    30061 tcaactaact tatctttaaa attgtatgac acttcacttt gcataagtag aaatcccata
    30121 tctttagata caaaactaat ttcattactg cttgaatctt tagacctatt aattatcatt
    30181 cctctaaaaa gttctttatc atctacgtag aaacaaactg tactagctat tggtatatct
    30241 atctgttgga aatttacatc aaatgatgat tgtattattg aaaactctag tgtccttgat
    30301 ggagatttat aatcacctga ccatgatacc ttgtctacta tgtcagttat gtcatatata
    30361 cttccgtttt ttatgtgtac ccaaatctta ataataaaaa atcacctgcc ttttcattta
    30421 attctatcag cagttattta taacttatta tctataatag ttattgtttt ggagttactt
    30481 ttggtatttt tattctttta tactctttta agtctaatgt aaaatacaca tctcgagtcc
    30541 catctcgttc ttcatagcta aagccctcaa ttatgacctc catatttata tttgtttctg
    30601 taattgtaaa tcttagtata aaaccttcct tcatccattt ttcaatctta tttacacaat
    30661 catatggtga tggaaaacct gtatagtcac agaaagtata gtctttattt ggaaaaaaac
    30721 ttgatattga aatgctttta agagctacac caccaaaagt tgctatttca cctattttga
    30781 gtatactaga agagtttaca gtagcttttc cattaatact aaaggaagat ggaaatactg
    30841 gaaatctaaa tctatcttcg gcttgtctaa gccacatttc cattataata cacctcccaa
    30901 ttgtgccatt tttatacttg aaactaattt tgatgtaact ttttctatat cagcttcttc
    30961 tctaatcact attgtatcag ctaacttagc tatgtttata tttccaccat tttttctgct
    31021 tctatattga tttgcttctt gtttagttaa aaccatttca ccttcatgga gtctggtttg
    31081 atagtcatta taaggaacat agttcaatcc aaatgcatta ttacctttct tacttgaata
    31141 agctttacca gaatcaccag cctttgttcc agcatatgga ttatctttgt aatttttttt
    31201 actcttttcc actagattta aaactgttcc tgtgatgctt ccatttttta tcttattaat
    31261 catagtttgc caccatttgc atatatcttt aactatttta catactttat ctaatatgtt
    31321 aaaaaaagct cccaatattg gctctataat tttccatcca gtttccaaca gaggtcctaa
    31381 ggtctcccat acagttttcc aaataacacc cagtgtctct ataaaattgt ttatttgagg
    31441 tgcatattca cctaaaaaag taaaaattct tgtgataata ggactaacag cttcaactac
    31501 aggcccgatt aattggaatg ctgtagttac tattgtacca aatatactta ttaatccttg
    31561 tgcaattggt gatttattgt ttatactatc tagtaaatta tttactaagt ttactactgg
    31621 aagtaatgaa tctattaatg ctgtaaaatc gatattttgt aatagtgtac ttcctgcgtt
    31681 aacaattgct tttggtgcat tccctatcat tgttgctgca tgattttcaa aacgttcttg
    31741 cttattttca accatcattt tacgcttttc agcctcatta gaaattcgat attgagtaga
    31801 atcagaaaac tcttttccat tttttacact tctagattgc atctggtcat atttgttgcc
    31861 aataattatc ttaccattca ttacattgcc tattttacca gccatttttt cagataagcc
    31921 agctaaattg cctaatccac tttctataaa agtttttatt ttctttagtc cttgacctaa
    31981 aatatcatca aaaggtttaa caatactctt caaaccattg ttaatgctcg tttgagctcc
    32041 tgatattata cctggtattg tattactcat ttctttagct aatcccatct gctttacact
    32101 tataccttcc aatgcttcat caaaagtttt ataattttct cctaccagag tattaagaga
    32161 ttccatattt ccttttgctg cacttaagaa agctgctgat acatcttgtt cactacttgt
    32221 attcatattt aaagctctaa catctaccat agcctgagtt atatcttttt cttttttacc
    32281 accagtaata ttactaatca ttttagcttt agttcccatt gcaacaacat cttcagggtc
    32341 aaaaggtgtc ttatctgcta tatctaacaa atcttcataa taattatcaa catctctttg
    32401 attaactttt ccagtatttc ttttcattac actttctaga acaactttct gattttgttc
    32461 ctgaccaagt aagtcaacac cctttttcac agcgacacct atagtagcac catttgtcaa
    32521 atcagatatc ttccctttta ttgaaccaag tattccactt gccatatcct ttacagctat
    32581 tgcacctctc cataccttac ctgcaaacaa tctcaatttt ggaaatactt tacttataat
    32641 gctacttgcc atatctttta tagctattac accactccaa aaagtatttt taaacaacat
    32701 taacttaggc aaaattctac ccatagcact gcttaccata tctttaagat taatacttcc
    32761 ttgataagga cgtttctcta ctcgttttaa atttcccaag atactactta tcactctctt
    32821 agtcatatct tttgctttta ttacaatctt ccaaaccttg ccaccaatct tttttaaatt
    32881 tccaagtaat ttatttttag ttttttctat ttcatcactt ttagctttaa atattaatat
    32941 aatcataata tttgaattta actcattttt cttttgaatc aacttattaa aataatcaat
    33001 aatcatagct atagttattg ctagtctcat aatacttcca tttaaacttc caataactcc
    33061 tgaaacagca tttatgctag aacttaattt tgtcatagtt gaacttacac tgctagcggt
    33121 agtctgaact tttcttaacg aagaatttaa actgtttact ctactacttg cacttccaat
    33181 agaatttttt atgctactag ccatcacatt agtagaaagt gaaacagcca cagttgctct
    33241 cctaaaatta tcagttactg atataatttg attcattttt ggtgtgtaat tatctgtaag
    33301 atacatattt ataccttctt ctcttacatt tcccattaca taccacctcc tgtcaaagga
    33361 cacactccat atccatgttt cattttttca acttcttcct caatttctaa ggcgaaaaaa
    33421 gcttcaataa gctttaattc gcctttatcc attgcataaa aaagggacgg ccttatacct
    33481 tttttcttcc aataatagta cattattgta gtcttggtat ccgtccctat tagtttttta
    33541 cctcttctat cactgcattt ttaccataac ccataacatc ttgaagtatt ctatataagt
    33601 tggtaatttc acctggtagt aataattttc ttgccaattc ttttggtgtt ggagccttaa
    33661 atttatccat tagctcctta tttttaaata aaagctttcc atctaaatca caaacacctt
    33721 ctataattgt aagcatctgc atctccggta agtctatatc cgttttcata ttttcatcaa
    33781 ttttaagaca agtattttgt atctcttcat ctttacttat tgttaaagct ctacacatta
    33841 ctttaaattc ctgcccaaat atagttgata atctcttaag ttcaactata gtgctcggtc
    33901 tttctaagtt ttctgtatct gaatttaaaa gtaaatctac tatatttata ttttttgata
    33961 atccattttc actcattatt atttgctcct ttatcaatca aattcaatca actaactttt
    34021 ttacttatta actatctgaa tttattaagt atatacttac taaatatcat tataaaacca
    34081 aatataaaac taaattatat caagaaaatc atactcagta aatgtgaaag gtgcttctat
    34141 ctctcctttt actccaacct cccaatcagc taaagttaaa tcatcaaacg ctatattttt
    34201 tactgctatt ctttctgctc cataagaatc tgggtcattt aatttagata tagcaacaaa
    34261 tctaggttca gaacctctct ttatcttttc tccaattaac ttatgcattc ttgaactaac
    34321 atggtataga gttattgaac cttttccttt atatcccata tactttgtat cagtacccat
    34381 ttgacctgct attataatat cctcttttgt aaattccatc tttgcttgaa acttctttac
    34441 ttctgctact ttgtttccat caagccaaag ttctccccaa gtaccactca ttacatttct
    34501 agcttccata tttgccatat taataatcct cctatatttc tattgataaa tctatatctt
    34561 ccatagcatc aagtactttt atttttgctt ttaaaaatac tttagaacct gtgttagctt
    34621 cttttatttc ttgtaatgtc atataagata aatctactcc ttttgatttt aaatacgatt
    34681 tttgtgcttc aaaatctatt tcaacagtag aatcagattc tataagtgca gatttttcta
    34741 attcttctaa ataacttttt atagctacta tcaataaaca tttgttgtca taactgttag
    34801 taacctttcc tatatagtca tctattatca cctttcttat atcactatgt ataatatcta
    34861 aagtgtcaac tatttttatt ttctggaaca tttctccttt ttctgctgtt aattcagtta
    34921 aagaatttac tcctctagca attcttatag ctcctgcttc tttaataagt ataagctctc
    34981 ctttattaac ccttgattct gcatcaactt tagtcatctt aggtatatcg actacatcac
    35041 taagcttagt ataggttact gattgactta aaggtgtacc tgctataagc ccagctaccc
    35101 tacttgtaaa ctcatcaaca ctgtatttct tttctccaac taacacatct tctgtagtaa
    35161 aattaattat cccttcatga tttcctacaa cttttcctaa tatagcttta accttaacct
    35221 tatctatatc tctaagttta attatccaat tttttatagc agtcttatca gcttctactg
    35281 cttttggcat acatagataa ttaaattcct tagtctctag gaagtctaat gcagtttgaa
    35341 tatctgcttc tccttctatt acataaacta ataacttatt tggagtgtta acatttccca
    35401 tcaaagctaa atttatatat tctttatttt cagcagataa atcaactggt atatcctctt
    35461 tttcatgtat ctcgttaaga cctaatgcct tagcatcttt aagcaccatt gcaattattc
    35521 ctctagctga acgttcttta acagttgtag ctagctcctt aaatgatatg ttgatacttg
    35581 gtaatcctat agccatacta tcactctcct aaatttaaat tgatttcttc catattttca
    35641 tatactacat cttcttcata tacactatcc agatagttta gtgtaaccga aaaattcaat
    35701 gcataaattg actcttcttt tttaatactt tgttctagct ttgttatatt taattttctg
    35761 tctgttacct ttatatttct gttaaatagc ttatttaatt catctgaaat ttcatacatt
    35821 ctaatactct ttgactgtgg ttttggtaag tattttatag agataacaat atctttgtta
    35881 ttcgtattta acattatagg tgtacaaata actgataata tacttacaaa aaaacaagac
    35941 tcttcagagc cttgcacact ttcatcatca ataaaaatac tttcagtaaa gttttttttg
    36001 agaatctcaa taattgtttc taatatttcc ttgtatttaa gcaaataatc accacactat
    36061 cactaaatat tcttgcttaa tctgataatc ttactataaa tgatataagt catttttaaa
    36121 tatacatctc atttattcct gtcaagtatc ttctatattt ctaggtaata ttattctttt
    36181 caatctatca cctccttcaa tatggatttc tttcacaata acattatctc acatatttta
    36241 ttacctattg ttgcaatgtt gtatataaag tgttgtaaaa ttgtcctatt ttttataata
    36301 tctcactcaa tttaactatc atctttttct ttaagttgta gcaggatgtt ctatctaagt
    36361 gtagtttcat agaaatatac gtcatattgt tttttgtctt actattataa aaaagattaa
    36421 aaaattccat ttctatatca tttagacatg ttagagcatt ttctatcttc tctttttcta
    36481 tttctatatc ttctttttct atttttaatt ttcttaatcg ttcctccctt ttcaacacct
    36541 cattttctac agaggaactt atattatatg tgttactagt cttttctgta tataccattg
    36601 ctccacatcc agagtattca ttttcatatc tttttatatc taaattaatg ttctttatct
    36661 ttatatccaa atacttgtaa ttatgtaatc tttcatctgt ttctttaaat aatatatctt
    36721 ttttgttttc catagtattt ctccttttta tatatttatt gttagcttca aatttacaac
    36781 aatcttttta gtactaattt ttttattaat cttaataatt ttaagaacat tttgttctat
    36841 aactgtataa aaaaaacttc ttctatattt tgttctaaaa tgctagaaat cttcttggcc
    36901 ataagaagcc caggattttt tatacctctt tctatttgag atatataatc atttgtaatt
    36961 ccagcctttc tagcaagttg aatttgtgtc attcctttgc attttctata atatttcaaa
    37021 ttatttttaa acattgtttt atacctccat tactattaag aatattttgt tctcctatat
    37081 ttagattata tagaattatt tgttctttat caatagattt tagaaaaaaa tattctttta
    37141 tactttacta gttttatttt ttagaacaat ttgttataat ctatacataa ggtggtgtta
    37201 taatgtttgc caaaagatta agagaactta gaaaggaatt tggattgact caaagagaac
    37261 ttggagaaaa agtaggtgtt tcccaaagag tcttaggata ctatgaaaca gaaaacagat
    37321 ttcctgatga acacatttta aataaattgg ctgatgtatt tgacgtatca gttgattacc
    37381 ttcttggaag aaccctagtt aaggaaaata ttgatacagt agctgcacat agaaaaaatc
    37441 cacatgaaga gttacctgaa gaagcacaag aacaacttaa tgattatata gaatttttac
    37501 taaataaata taaaaaaaaa taaaccatat gatgagcagt ctatttttct gctcttttaa
    37561 tataactcaa aggcaaacat acattctatt ttagggggat ttttatgaat aatttggata
    37621 agctttttga attagcttct caagaagaga taataattca ttataccact tatattgcag
    37681 gtgatttaga aggactatat ataaataagc acggtatgaa aatcatatca ttactcagta
    37741 atttaaaaca aaatacaaaa aaattgacat ccatcttagc tgaagaactt ggacatcatt
    37801 ttactagtct tggatactat gtatcctctt ataatgatta ttatacaaaa atcattatag
    37861 ataagtgtga aaataaagcc ttaaaatggg cttgtgagtt cttaattact gaagaggata
    37921 taataggcat aattaattct ggcgtcactt gcgtttatga aatggcagat atacttaatg
    37981 tggaaattaa tttttttcaa aaaagattag aatttttatc attaaaaaaa cagtctttac
    38041 aacttggaaa taataaatat ttaatattaa ctaatttgcc gtatttctac atatttgatc
    38101 caatttcata ataaaaatag tagtaatttt aactggctta ccagtaaaat ctatatattt
    38161 attttaacaa attttggttt ctataaattc aatattaata tataataatg atatcatatt
    38221 atttaaaatt ctaaaaacaa atatatgctt ttgttaagaa aggttgtttg atgaatgtat
    38281 aaagaaaagt tgtttgataa ttattttaag ttcttagcat tatttttttg gccaataatg
    38341 tggtataagt ggatagtaat ttcaaatggt actcttgaaa atatgttatt taccacctac
    38401 gcaattattg caattgtttt catcatttta tattcagtat ctatgataaa atataaagac
    38461 attacacaaa tagatttctt ttacaggata agtactctac ttgcttttat atttacactt
    38521 tttagcttct taatataccc aaagagttta tttttcttgt atttaaagat tatttttact
    38581 ggaatatacc tttattattc aatagtaaaa acacttaaat ttaaagatga cgagggtgta
    38641 gtgggtatta tgagctcact tttattaatt gtaataactt tattctacta aatttaattc
    38701 tatttgactt aatatggctt taaatattag acatctacat tgttttctga tattatttaa
    38761 gatggtagtt ttatattaat aatgaaatac actatgaagg agttaactat tatggaaaaa
    38821 aaagttttag caacagttgg agaaaaagag ataactaata ttgacattga aaatgcatta
    38881 aaatcattag acccttatca agctatgcaa tttaaaactg aagaaggaaa aaaacactta
    38941 ctaaatgatt tagtaaatca agaattattc ttcttagatg ctaaggaaga aaaattagat
    39001 gaagaagaaa tatttaagtt agaaatgaaa aagatagaag aaaatgtatt aaagcaattt
    39061 gctataaata aagttttatc tagtgttaat gtaacagaag atgaaaaagt aaaattcttt
    39121 gaagctaata agagttcttt ctctaaacca gaaagtgcaa ctgctaaaca tatattagta
    39181 gatagtgatg aaaaagctaa agaaatactt gctcaaataa aatcagaaga aatatctttt
    39241 gaagatgcag ctcttaaaca ttcttcttgc ccatctaaag atatgggtgg tgatttagga
    39301 acattcggta gaggacaaat ggttcctgaa tttgaagaag ctgtattctc tatggctaaa
    39361 ggtgaagtaa gtgagccagt taaaactcaa tttggatatc atataataaa attagaagac
    39421 cttcaagaaa gtacagagtc tacatttgat gaagttaagg ctgaggttga aaaaagttta
    39481 ttatatcaaa aacaaaatga agtttatgga aataagataa acgctcttaa tgctaagtat
    39541 ggaaaccttg taaaatacaa cgattaatca taaagtaatg tataaaaaag ctgtcttttc
    39601 acaatattta ttataaaaga cagcttttta tattaaagtt taaatatcca gcttaatatt
    39661 atcattcttt tattgcaaag aactcagctt aaaagtttaa ataagatttt taattatcat
    39721 tagctaaatt tttatatcca cgtattatct cataacaata acctattaca tctcttcttt
    39781 ttattatccc aataaatgtg tcttggtcat ctataacagg aatgaagttt tgatttaaag
    39841 attttataac taaatcttca atatctgcgt tgatggaaac aggagaatta tccatttttc
    39901 tattaatatc tgtcactgga acatcttcca atccctttaa gtccagattt aaatcgtttt
    39961 ttaatgtcca taaaaaatca ccttctgtta tagttccaac atatttacca tccttactta
    40021 ttattggaat tgcagagtat ttgtgatatt ccattttttc tagagcttgc cttatagtgt
    40081 agtcttcata tatatatgct acttcacttt ttggtgttag aaagaataat atgttcatta
    40141 cactacccac cgttactgaa aacttataaa tattattcaa tatttataat gttacttttt
    40201 tgcagtaact ttctcctttc ttattttcta agtattctta atactatacg ctgtaacaag
    40261 cgtttttctt ttggtcattt taatcttatt atgctttatt aatattttta tatggtctat
    40321 cagtatcact tattctcttt ttagaaaatt cactaaaaat taattgactt aaacttagcc
    40381 ataataattt tactctacac tattaatttt aacatattta tctcattatt aatgatttta
    40441 ttttaataat tttaatttat tttgatatta attttattta caaaaactga aaaaaccgct
    40501 aaatgattag cggcttatct ctcatattac tagaacacta atatatatga atttttaatt
    40561 ataatctaac tacgttagca gcttgaggac ctttagctcc atctactact tcgaactcaa
    40621 cgctttgtcc ttcttctaat gttttgtatc catctccagt tatagctgag aaatgtacga
    40681 aaacatctcc ttcaccttct acagatatga atccaaatcc tttttcgttg ttaaaccatt
    40741 taactacacc agttttcatt aataaatcct cctaaaaaaa cacaaatatt tagctactct
    40801 tttctgtaac cttattacta ctctatcata tatagctatg tttgtcaatt attattattt
    40861 ttatacatta ataatcaatt tttttacttt ataattttaa atcattatta tcttttacac
    40921 tttatttttc tttatatttt ttcaataatt tatttttatt atatggaatc ttatggactt
    40981 atcactccaa cctaaatccc atggcaccaa cattctatcc gtattatggc aagttaccaa
    41041 aggatatcct cttgagtcta atcctgtcac tgtcgaaaca tgtgttattc tgcctttctt
    41101 ctcataacca acaaaatctc ctggacgtaa attataagct tctttgtata cttcttcata
    41161 agaaccttta gcaattaatg aacctcttcc actatacagt gcataatttt taaaagcttg
    41221 tgcatttacc catgcttttg tacctgctct ttcactataa ttccaaattg agttcttttt
    41281 gaaccttcca ctttcaaaca ttatctgtga tgcaaagttt gcacaatctc caccttctgg
    41341 attataatcc ctatagtttg gattaaattt cataccatgt tcttcatcag ctgcggctcc
    41401 acagtattta tgtgcataat ctattgcctt tttttgctca tctgtaagct gtaactctac
    41461 accatcttgt tgaccaatat agtttcttat atcatcagat tttatatttt ctaaatttaa
    41521 agagtctgca aaagggtctg tataccactc tttagttatg acatattgat tatctataat
    41581 ttttacattt agataatgat atgtacctat tctaaaggca ttttctttgt ccttttgatt
    41641 ttcatatgaa tacttatatt cagtcgatac tgaacagatt ataccataga ggtctttttc
    41701 tttttccttt atttttttaa tcttaaccct tgtctttata tctttaaatt ttacgccttg
    41761 cttactagac cagttttcta aatacttcat cttttttact tcattttcat aagcccaaag
    41821 accaaatttt ttatctgtct cataaagacc ttttaaaata ttttcatttt tctctaatat
    41881 tgccttattt ctgtagtcaa ataagttttc aagcaatagt ttatattggt cttttacatc
    41941 atcaccttca atatttgcag attccatgac tttttcttcc atatttttca tacttaaatc
    42001 attgattaaa aatacacctg ctgtagtagc taaagataaa aatacaaatg ccattatacc
    42061 tttgatgtac ttattcttat ttttcagtat tttcaaagct acacctccca aatagtacac
    42121 cttacaaatc aaattacaaa gaatattctg tcacatacaa taattttcat acttaataat
    42181 tctatatttt attatgtcca atattaaaaa ttaattgtat caatacactg ttttttttac
    42241 aattttttat tttataatct acttttttct tttttttata gtaatatata tgtatataaa
    42301 tattttggta tcattataat taatacatac taatggaggg ttttttatgc aaaaaaaagt
    42361 cgcagcaatt aatgacttat ctggaatagg aaaatgctca ttatcagttg ctatacctat
    42421 actttcagca ttaaaggttc aatgttgtcc atttccaaca gccatacttt caagtcaaac
    42481 tggttatcct gaatttactt ttttagactt tactgatgaa atggtaaaat attcaaatgt
    42541 ttggaaaaat ttaaaagtcc actttgatag tatatacagt ggttttttgg gttcaaagca
    42601 tcaaattgag atagtggcta attttataaa tgattatccc aatgctttta ttgtagttga
    42661 ccctgtgatg ggtgataatg gagttatgta tccaatattc acagaagaga tgagacaaga
    42721 aattaaagaa ttagttaaac attcagattt gactactcct aatttaacag aagcttgttt
    42781 cttaactgga aatgattata ctaaaagtga ttataataga gatgaactta tatatattgc
    42841 aaaatctgta tctgatttag gacctagcaa agtcgttatt actggtatat tagaagatga
    42901 aaacatacta aacttagctt atgatagaga taatgaccat gtattcttta catcagttaa
    42961 atacaataat tgttcttata gcggaactgg agatatattc acatctatac tttgtggtat
    43021 gttagtaaat aaacatgatt taggagtagc tgttaatact gcaacagatt ttatatataa
    43081 aactataaat tacacatctc aatttgatac tgatagaaat gatggagtta tgtttgaaaa
    43141 ctttttatca gatttaacta atatataaaa tcaaaaaaat tatccatttt ataaatacct
    43201 atgaatcata aaatttaggt atttacaaaa tggatattat ttactaaact attggtattt
    43261 caattgttac catagcacca ccagttttta tagagttttc aatatgtaaa atcccattat
    43321 gtttttgtac aatattattt acaatataaa gacccattcc ataatgagat ttagagtttc
    43381 tacttaaatc tcctgtataa aattgtcctg tagcctttga caaatctgct ttagaaaaac
    43441 cacttccaca atctgtaatc acaaatttta agtatttatt acttactgat acacttatgt
    43501 atagtttact ctggcttggt gaataatcta tagcatttga aattacattc ataatagctc
    43561 taaataacaa ttctttatct atagttatac tttcaggcaa attaccttgt ttagaaaatt
    43621 caacattcaa gttttgtgta cgagctaatg cttctatttg aatcaagata tcttctataa
    43681 attcacttac attaattact tctttattta atatataacc agtctctgtt tttgatatct
    43741 ctattagtgt attaatatat ttttctattt gggtggcacc ttttgctata tattccatgt
    43801 attctgataa ctctggattt tcatttattt caattagtaa gtcagtattt ccatgtataa
    43861 tagtcaatgg tgtttttata tcatgtgcta atgcagatat ctgttcctta cgattctctt
    43921 ctaacatcca ctgtttttct aaagaatctt ttaaagctaa cttcatttta tccatagaaa
    43981 aaagaacatt attgatttca cggattttac tagattctat aacaaaatct aaatcctgct
    44041 gttctatttt ttctgtagta ttttttaaaa gttccatttc agcatttaat tttttgccaa
    44101 ataattttga tagtataata atttcaataa gaaatattat actaaaaata atcattccta
    44161 atagttctgg ctttggtaaa tatgtcctta aagttggaga aacaaattct gctactatac
    44221 tataacgtat tatacaaact tcattttttc tggacaattt gatatagttt tttgatgacg
    44281 taatagtcct tctttcaaat cctcgcatca aatcccatac ttcttttgac tcattcttat
    44341 tgaaagttcc tgaaattact ttgccattta aagtatacac tccatactca cataaatctg
    44401 gaattaaatc tttagttact tgtttacttg aaataatctt atctttattt tcatagattt
    44461 gtttttccac ataattagca ggtaatatta tatttaatga taataatata ggaaatgata
    44521 atatcaatat agatactaag acaacggtta tagcaaagaa taaagatgaa tatcttacaa
    44581 aaacagttct aagtgttaca atctttttat tattaaactt tttacctaca agtatcctca
    44641 ccacatcctc caaactttaa ttagtttata gttaattata aactagcttt tgatttgatt
    44701 attttttatt tttatcacta aactatattc taacataata atataagtaa atagtaactt
    44761 ttttattttt attcttcaga ctttttaccc tcccatttag aaaaccaaat acataaaatt
    44821 ataaatgcta aaatggttac aaatccacat actaatccta ttgattgata tcctttaatt
    44881 gattcgactt taatatattc aattgtctta gaacttgaaa aagatgacca tatacttata
    44941 aatctaacac cccatacaca aggaatataa ggccaaattc tagcacctaa tccagttagc
    45001 aacactgctg agagtaaagt ttctactata cccactccaa ttgaggctcc ttttccaaat
    45061 cttaaactta agaataaatg taatatatat acaaataaat tactcccaac taaaatcata
    45121 attgaaatca tataaaaatc taatgtaaaa taaacctcat tagaaatact agatacacca
    45181 aatttaaaac ctaatacaga tactagtgtt gctccaaaac caaggcagat taatattata
    45241 tacttactta taagtgtaag atattttgga ttagatgatg ttaacatatg tttataactt
    45301 cctgaataat actcttgttc aatacataat gaacacataa tacttgataa taaaggaaaa
    45361 gctattgcta taacttgtaa atatgcagct atctttgata tatcatcagc acttgaatac
    45421 ttataatatc ctaagcacaa tcccatgcct attaaagcaa tgcaaaagtg cattagtatt
    45481 ataaaagttc gctttaattt tattaaatca gaacaaaaaa ttcttactag ttgtgtcata
    45541 ttacttcacc tctctattat tgaaccaagc atttgtagct aaagaaagta atataaataa
    45601 aacaatagat attaacactc ctggcaaaat tacattacta tttaaaaact cacttccttc
    45661 tggtacagga agtccatttg gtagaatatg caatacagga gccattaatc gtggagtaat
    45721 tgaatatgga aatgcatacc acatttctgt agtcgaaaaa acggcaactc caattatagt
    45781 aaaaaacatg tttattataa ttgtaataaa cataccaaat tttgatgcca aaaacataca
    45841 aagaggaatc tgccacataa ataataaaac taataatatg gttccaataa tattttgact
    45901 tactgaaata acttccttat ataacatccc aacaagaaat atacctatca taaaaataat
    45961 tgaggttata aaacaacata taatgcaata aagtattttt ccagtccaaa catctttttt
    46021 ttcaattggc aatgataaaa ttccacgata cttctttttt tcatcttttt gtactattag
    46081 agaagaaatc aatgttaata cacctggaag aaacatagtg taccaccaat tataaacacc
    46141 attttgtcct ccaccaagta aaaatgtaat gattatagaa aatattgggg ctatccatat
    46201 caatctaatt ccaaaagttc ttttcatttt ttgaaattct gctctaacta atttttccat
    46261 attaatttcc tctctttcta ttttcagcta caactttcat aaaaagttct tctaaatttt
    46321 gctcttttga tacttctcct tgatatccta gtactccatt agaaattatt ccaatttcat
    46381 ctacaacttg ttcaacctca gacaagatat ggcttgataa aataactgta atcccttgct
    46441 ttgggaaaga acgaatcaat tctcttagtt cttcaatacc tatagggtca agaccatttg
    46501 tgggttcatc taagattaaa agctttgggt gatttaataa tgcaatagca ataccaagac
    46561 gttgcttcat tcctagagaa aattgacctg aacgcttttt aaaagtatct cttaggtcga
    46621 caatctttaa aacttcttca atgcgagaat caggaagacc aagtaaagta gtatgaactt
    46681 ttaaattttc tcttgctgtt aaattctcat atatagcagg agactcaata agagaaccaa
    46741 tatttgataa atcctttcta gtccatctat gcccctcaaa cattattttt cctgaagttg
    46801 gatttaaaat tccagttatc atttttaata aagtggattt accagctccg tttggtccta
    46861 gtaatccata aatagaatta tgtttaattg ctattgaaat attatcaact acaacctcat
    46921 ccttaaactt tttagaaact ccttttacct ccaaaatata attactcata attttcatcc
    46981 tttcttttga caaacttatt tacaagaatc attctaaatc ataaatataa ggattttata
    47041 agaattaaaa aatcacaaaa aaagatgtgt taaaaaacac atcttttttt atcttaactc
    47101 tattaatttc ccattgtagt atatttctac ttcttcaatc caaatactat tatcttcttc
    47161 ttggatgaca tttttaataa tattatttat agtgtatatt atattttgct cattcaaata
    47221 atcatctttc caagaaactc tacttttttc atctggttct atcaagttta ttttaacaag
    47281 attatctttt tccttaactt ccacttccat gtctaaacca ttaaaatact cctttgataa
    47341 tgtgtctaag attaatcgta gtttatcttt aagtgtactt tcttcatcaa tctgtaagta
    47401 ctttactatc ttaccatcct ttgaaattat ctctatgggt attttagtag tattcttaga
    47461 attaatcatt ttactagttg tttcttctct cacatcatca tccttcttta caactgctga
    47521 ctctttagaa tcaactagct caactgcgct gatgctataa tccatacatg ctgtactcaa
    47581 taaagtagta gcaattatta aaaaacttac tgctatttta ttcataagta tccctctcct
    47641 aattctcttt cgatacttat tataccatag tatagattat atttttatac ttttaaatca
    47701 atttataaat tattatagta aattatttac aattctaatc tatttgaatt tatttttata
    47761 gtatattctt tctctaaatt cgacagattt ttattcaaaa gcccttttat ttccctcgta
    47821 tttctatact tgttgctatg ttcttcacta tgttcataat catatgaatc attgtcatat
    47881 tcttcatcat tattaaattc ttcgattact tcttttgact ttatatcaca attaaattta
    47941 tacttattta ctggtatatc aagtcttacc ttagaatttg gaacttctat atccagatac
    48001 ttagcaattg gcatattaga atataagtta aattcattat cataatttga gtgtctacct
    48061 attttctcct ttatgtttac attttcaggt atatattctt ctatattatt gataaataca
    48121 ccttcatcct ttgaagatat atccaatgac tctgattcta tatttacatt tttaacacct
    48181 aaaattgact ttgttgatag atgaagattg ctattagata ttaagtttaa gttttctaat
    48241 ctatttactt tattttctgt tatagctctg ctaaaacctc cagaagaagt tttatatgtt
    48301 atattatttg aatctacatc atcagatata aataagtacg aacttatact tgatgcattt
    48361 atatctacat tatttggtac atatattcta atttctctat aatcacgtga aaacatacta
    48421 tttatagatg tatttaataa gtggtcgaaa cttctaatct ttttaatttt attttcagat
    48481 tttctttcgc cttttacaac taaagtctta tcttgtaggg ttacactgta tttgtaaaat
    48541 tcattattcc ctcttgttgt cacacgcaca tcttcatttg aactttttct tatcataaca
    48601 tttatattgt caacagatac atctaacttg tcaatattta ctttttcttg ataaagtttg
    48661 ttttctttgt ttaattcagc ttccttttct aaaccaaagt ttattaagct tggcataact
    48721 aaaatccctg accatgttgt ccctattata cctattacca ataataccgc agcaaatatg
    48781 gcaagtttct tattcatatt tatcttctcc tttcttattt tctatatctt taaggtcctt
    48841 atatatcgcc tcaaaattta tactatcatc attatattca tcagtttctt ttatgctttc
    48901 ttcttctcta ttgtagtaat cattattata tgaatcttca ttaatgtctt tattcaaatc
    48961 ttcatcattt aaagtagctt tttttctttt ttccatcttt tcatttgcct ttatataaat
    49021 attttttctc tttatccaat gtatataatt atttagtaat tgtttaccta gttttatggc
    49081 aaatagtaaa agttgccatg caagtatctc aatccctaca aatgctatgg ttgcgaaaat
    49141 acaaaacatc ataacatctg gaaccattgc tataaattta atcacaaatg gaattaatat
    49201 aacaaaagca attaatgaac atactaatgc aatagtaaca gatacaatag cacatacaaa
    49261 cgcaaatgct ggaacaatta gaaataaaga taacactact aataatccct ttataacttg
    49321 tctagataat ccccctacac cttcttctat acttggtgta agtttatcat ttatattttc
    49381 ttttgattta ttgtactttt ccttagcttc atctttcaag atagagtaat ccttttttat
    49441 atttgattta aacctactaa atttaccatt taatatatct gtactcatta tttttccatc
    49501 tatattttct tttgatttac tatacttttc tttagcttca tcttttaaga tagaataacc
    49561 ctttttcata tctgatttaa atttactaaa cttatcattt aaaacatctg tatttattat
    49621 tttttcctct tgaatatttt tttctttggt ctgtaggata agttctttag ctatagattt
    49681 tggtgaacca agtgatgata ttatctccat atcactcttt ccttctataa gaccatctac
    49741 aaaatattct tcataatctc tcaatatatc attaacttcg ctctcagaaa aatctttttt
    49801 tagataatcc tttaaaattt caagaaactc agttttattc aagctcatta cctcccttat
    49861 cttcacttat taaaatttca acagccttta caaattctct ccactcttcc atcagattgg
    49921 ataaattttc ttttcctagt gaagttattc tatagtattt tctagcagga ccttcattag
    49981 attctagtat atatgtttcg aaatacaaat cttttgtaag tcttcttaga ataggatata
    50041 ttgttccttc atttacatct attactttag aaatgttatg tactatctca tatccataca
    50101 tatctttctt agatatgaga gcaagtacac aaatttctaa gacacccttt ctaaattgtg
    50161 tgttcatata ctccatcctt tctttaaata cacagtattg tgttttacaa ggtactatgt
    50221 taaaacttct tatatcccca taatagcaca gtactatgtt ctgcacaata gttattttta
    50281 aatattattg ttgattaaat ccttctataa atgtataata ctctaatcag atatatttta
    50341 tgaaagtggt gataagttgg ctaaaatgaa agatgataaa acaatattaa tcttaactgc
    50401 tcaatttgga gctggacata taagtgctgc aaaagcagtc aaggaatcta ttattgaaaa
    50461 atacagtaac tataatgttg ttatacaaaa ctttataaat gcaagtattc ccatgatgaa
    50521 taagcctatg gtaaaacttt atgaaaacaa tacaaaatat actcctggat tatataatta
    50581 ctattattac tttaagaaat cgtttgattc aagacatgat ttttctcata aattatatac
    50641 acctaaactt tctgagtata ttgctgatat aaatccagat ttgattatct ctacatttcc
    50701 actagctgct gcttgtgtaa ataactttaa gataaaaaat cctgatataa atatacctac
    50761 acttacagtt ataacagatg ttgtagatag tatggaatgg gtttttgaaa atacagactt
    50821 gtattttgtt ccttctcctg aaataaaaaa tagatttttt cagaaaggaa taaatccaga
    50881 ttccataaag gttactggag ttcctgtaga taagagattt caaattgaaa gtaaagaaat
    50941 atgttgtgat aaatatagac tattgcttct aggtggagga agaggtctat ttgatataga
    51001 tgaggacttt atgcattgga tagatgaatt tattgaagaa catagtgatt ctatagaaat
    51061 tacaatagtt actggtaaaa acaaaaaatt atacgacaat ctaactcata aaaaacctct
    51121 aaaaaatatt aaagtacttg gatttgtaaa cgacatgtat aatctaataa gagagtgtga
    51181 cctaatgcta actaagcctg gaggagcaac tatatttgaa gctattcaat cacaaactcc
    51241 agtacttgta aaaatgccaa aagttggaca agaaattgaa aatgctaaat ttataataga
    51301 taaggggctt ggcatgattt atagtgatga cctagaccta aaaaacatat tttataggtt
    51361 agtatcaaat gaatttgatt ctataataaa ctttatgaaa aaaaatttag aggaatttaa
    51421 aacagtcatt catccagaga aaattgctga ttatatatca gaattaatag ataaacatta
    51481 cagttaaatt gtgctattac aaaattttaa acaaaaaatt aaaggcaatc aagtatgctt
    51541 ttacacttga gaatttactc atagtgctta ataatacatg attgccttta tcatatcaaa
    51601 atcaaattgt tatcttagta tatatttttc ttgtactgca tatatagttg ttgtatttct
    51661 tccatacttc tctttaactc tatttgtaaa tctgatgcag cctgataatc tttattttct
    51721 acagcttctc tcactctagt aaatagcgac tctctagtta aagtatcctt cataatattg
    51781 aactttaaat ctgatattga gttccaaact acttcatcaa gtgcatccat attttctttt
    51841 gtgtgtatct tgacaatact tcttattttt tgaatccctt cttctattat tctagttttt
    51901 aatttctttt gccacttatc tatagctcca atcttaaagg attctattat actatcagta
    51961 aatacatcac cttgttttaa agattttagt tttgaagcat atatttctaa gtttttcata
    52021 ttttcataaa ctgtagctgg tgggattcca aatcttgcat ttctttcttc caaactgtaa
    52081 tgttcaaata catcattttc atctctatat gctctatctt tttctaaata gaaactttct
    52141 tcacctacat ttttagataa ttctttttca agttcttttg tactaagacc actcttagct
    52201 gctgctttta taccatccaa cattgtttga taacaccctg ctattactaa atatgtgtta
    52261 gatagagggc ttggtgatct caactcaaat cttacagtct ttggattttt catatctctt
    52321 attaatccaa caagcactga cctatttctt gatggaatct cataactatg acctaaagat
    52381 gtaactatac atacaggagc ttcaaatcca ggaactaatc tattaaatcc atcattagaa
    52441 gctgttacaa ttgggtttaa aacttcatag ttgtaaagaa gtcccataag agccccatat
    52501 cccagttcac ttagataatc ttcttttaaa tcttttggag caaatagatt ttttatactt
    52561 ccatccttta acttcgcact tactcctaca tgtgtgtgtc caccacttcc tgctactcca
    52621 tgaattggtt ttgctttaaa tgtaacttct agtccatgtg atgtaaatac atcttctata
    52681 acatctctta ctaataactc attatctgct gcttgtaatg gtgtagaaaa cttccaagaa
    52741 acctctaatt gctccatagc atggtttgtt cttccatcta tactaataga actttgtatt
    52801 cctccaacct ctttgtgagc catttcaggg cttacaccta gtttttgaag tataattaaa
    52861 cttttctcta aacaagttct tataatacca tgagttcttt tccagtattg ctcttttagg
    52921 ctttgtgata catataattt ctctaggtca gctttatctt caggagtatt aacccagaac
    52981 tcaagctctg tagctgctgt taacattatt tcctctatat catctactga atctattcct
    53041 atgttattta ttacatgtgg atattctcta aatatttcat acattgattt tttaaaatac
    53101 ttatctgctt tttgaagtac tcctctagaa catacttttt tattatcatg tattaaaaat
    53161 gcaggtatct ttaatgttcc aacaggtaga cctacttctt cgtctatata ttccatatta
    53221 taatctacat accaatgaca acttttatcc ggcattaaat ctacttttgc attatttaat
    53281 gtagctatgt tataaagctc aacacttgaa ccatcagtct gtatagctga ttccaaaaac
    53341 ttatctatgt catccaaaaa taattctaca ggaatctttt catctgttgc attgcctcct
    53401 aaatcaaccc ccattaagga aacaaatctt atatttttat tgctttttag gatttctctt
    53461 aattcttcat tagtatgttt atctttctct attacgtata atagactatc catttttcca
    53521 ctctcccata atttttatgt aaattttatt ttgttaaata caagataaat ttcattttcc
    53581 ttacatttat gaatttttct tgcaaattat tcgtgtttta ttcgtgtttt tatttatatt
    53641 attatatatc aagtacaaaa agttcgcaat attttttatt ggaaaaaata ttatacatac
    53701 aatagtttaa catttttata aatatggggt atactaaaat aaaagtattt ttatttgaaa
    53761 atttaatttt att
//
LOCUS       DAMJVM010000018        48994 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.018, whole genome shotgun sequence.
ACCESSION   DAMJVM010000018 DAMJVM010000000
VERSION     DAMJVM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 48994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48994
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.018"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            <1..172
                     /locus_tag="PC523_001137"
     CDS             <1..172
                     /locus_tag="PC523_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HDF2474682.1"
                     /translation="RTFQQEYRDYLDLAKFLQLMPGIGAFVGFYVNGKLIDKLCETAV
                     SAYRLRIRELKL"
     gene            381..1532
                     /locus_tag="PC523_001138"
     CDS             381..1532
                     /locus_tag="PC523_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HDF2474683.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRAAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVKKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            1557..2312
                     /locus_tag="PC523_001139"
     CDS             1557..2312
                     /locus_tag="PC523_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HDF2474684.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGVYFLNNNVRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAQGHEPVNISSM
                     CTVFAESEIVSLLAQNISAGEVAAGILKSIANKSTSMLARGEIIDEVAFTGGLAKSKE
                     LVKMIEEILDKKIFIAEDTQIIGALGAAVIGFR"
     gene            2504..4891
                     /locus_tag="PC523_001140"
     CDS             2504..4891
                     /locus_tag="PC523_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HDF2474685.1"
                     /translation="MKRFTSKKVARFVALFLISILVMSTIPASADTSNTLNQIKKAEY
                     SETYKQYLNDVKNGRTEKYNGIIPNPYQLEGTQIKSKGRTAPTSYDPRKLGLMTSVKN
                     QENLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMDGALNYE
                     AMGYLTSCSGPKLEKDIPYNGKISKAQGATKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSAYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKSNFNSNI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGQKPSDDEKMYQHD
                     YASIVPLISNKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTTQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSKFYPCNFTIKAVTEKSSSESVPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGGSIVDALAATPFTSAINSPILLTQKEALNSKTKVEIQRLG
                     AKKVYLIGGENSISKETEQQLKSLNISTERISGSDRYKTSLLLAQKLNSIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNVFVVKNGIKSQNDLIDGLAVG
                     PLGAKTGSPVVLVGDKLADSQKEVLKNKTLEKVTQVGGGANQNAFKELVKLKSVK"
     gene            complement(5114..5347)
                     /locus_tag="PC523_001141"
     CDS             complement(5114..5347)
                     /locus_tag="PC523_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDF2474686.1"
                     /translation="MTTTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
     gene            5637..6227
                     /locus_tag="PC523_001142"
     CDS             5637..6227
                     /locus_tag="PC523_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2474687.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMELYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRLDKSITCEQLTDIFMKFYRMV
                     DSTSILNLMVNGEFEILVRKLPDAIIEEHFRHDDFEIGEIISCIPNAKNKDIERFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            6341..7186
                     /locus_tag="PC523_001143"
     CDS             6341..7186
                     /locus_tag="PC523_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2474688.1"
                     /translation="MIKVDDLSFSYTDKNFLQNINFEVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYKGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDKLLKSVGLENKANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVKYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            7183..7866
                     /locus_tag="PC523_001144"
     CDS             7183..7866
                     /locus_tag="PC523_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2474689.1"
                     /translation="MRLKNLVLGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMLIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLVDTKSYANFYPFNICISLISGDKNFIVINILILISMIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            7863..8579
                     /locus_tag="PC523_001145"
     CDS             7863..8579
                     /locus_tag="PC523_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2474690.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYLLMFDLLLAFITPFMFFFASTMVVLGEIDDSISRYLIVTPLGK
                     TGYLVSRFGIPGILAFIITMILLIFFSLTRISFLLNLSISLLVLLQSVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKVQYILFFLPTFWLSKAFKEDNYIYMFISI
                     IISLIWILLLFKKFSKKIVK"
     gene            8676..8939
                     /locus_tag="PC523_001146"
     CDS             8676..8939
                     /locus_tag="PC523_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2474691.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            8911..9366
                     /gene="def"
                     /locus_tag="PC523_001147"
     CDS             8911..9366
                     /gene="def"
                     /locus_tag="PC523_001147"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF2474692.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            9427..9888
                     /locus_tag="PC523_001148"
     CDS             9427..9888
                     /locus_tag="PC523_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HDF2474693.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHILVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTGILKWKNRNFANDISNEHSLDLENL
                     YDGWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWYGNIMF"
     gene            9938..10840
                     /locus_tag="PC523_001149"
     CDS             9938..10840
                     /locus_tag="PC523_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2474694.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSIRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINLLFRHAKECNVHYVLP
                     GTLYLRGITRGIFFDFVKKEFPELFDKLSILYSTGSLNRGYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            11209..12021
                     /locus_tag="PC523_001150"
     CDS             11209..12021
                     /locus_tag="PC523_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2474695.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPVKYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLFEIENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            13070..13831
                     /locus_tag="PC523_001151"
     CDS             13070..13831
                     /locus_tag="PC523_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2474696.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQCINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWSGKRVFIIATMALFSGDGSGVLSRMLKKY
                     GAITVGGLHVRMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKYGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNVYTDKIKINAEECIVCGNCISLCPMKNLVIKNDMIISN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            13909..14662
                     /locus_tag="PC523_001152"
                     /pseudo
     CDS             13909..14662
                     /locus_tag="PC523_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            14948..15367
                     /locus_tag="PC523_001153"
     CDS             14948..15367
                     /locus_tag="PC523_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HDF2474697.1"
                     /translation="MDKISGKLTVLFEEPFWIGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDKIGYKRMQRKVKKEQEKGSIGTKAQNALKLQY
                     EERKQDRKNLAKARKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(15468..15734)
                     /locus_tag="PC523_001154"
     CDS             complement(15468..15734)
                     /locus_tag="PC523_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDF2474698.1"
                     /translation="MKKFILIYFIVINFIAFFSMYIDKERAIKHKWRIKEATLMYIAV
                     IGGSIGSMIGMYSFRHKTKHIKFTFGIPFIIFLQFLLVYFYILK"
     gene            15961..16662
                     /locus_tag="PC523_001155"
     CDS             15961..16662
                     /locus_tag="PC523_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474699.1"
                     /translation="MKFVVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDIDFRNDDVYVRFSKKGDKFVPFGETDAELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            16744..17352
                     /locus_tag="PC523_001156"
     CDS             16744..17352
                     /locus_tag="PC523_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2474700.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSNE
                     QLKNIYLKNEEDSVDAVVLAGLHKSSKKLILIRQFRVPINDYIYELPAGLVDEGESID
                     VSVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDKFLEADE
                     EIEALLISQEEAKEILQSNHKIDTKAFLILQMFVSLGTKLFE"
     gene            17676..18140
                     /locus_tag="PC523_001157"
     CDS             17676..18140
                     /locus_tag="PC523_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HDF2474701.1"
                     /translation="MKKLVIILISIINVVSLTACKKSNESKYNNLNNKESGQTTIEME
                     LDKNYDTSDSFVNARLFCVSNDIDVLETEISFQMDGDSGIVEIKDNKTDETLWSNTWH
                     GRVDNDTFTISLANIQKEKEYAIWFTGIKINHAIVKVSFESNLVKEKEIPSK"
     gene            18528..19544
                     /gene="gap"
                     /locus_tag="PC523_001158"
     CDS             18528..19544
                     /gene="gap"
                     /locus_tag="PC523_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDF2474702.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEEEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            19940..21109
                     /locus_tag="PC523_001159"
     CDS             19940..21109
                     /locus_tag="PC523_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474703.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGKEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLIV"
     gene            21490..25743
                     /locus_tag="PC523_001160"
     CDS             21490..25743
                     /locus_tag="PC523_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HDF2474704.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYERFGNINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGAKQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEYSIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINDIYDILPKDVKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEINNAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANIQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMLERESKNIDVKNIKVNRIQYAKNNHITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAMYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKTSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEVFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            25892..26386
                     /locus_tag="PC523_001161"
     CDS             25892..26386
                     /locus_tag="PC523_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDF2474705.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDDFIVSVYIGDEKFNNLKNEYIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKNIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            26502..26879
                     /locus_tag="PC523_001162"
     CDS             26502..26879
                     /locus_tag="PC523_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HDF2474706.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTRAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            27086..27526
                     /locus_tag="PC523_001163"
     CDS             27086..27526
                     /locus_tag="PC523_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HDF2474707.1"
                     /translation="MDNINLLINRLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMINNENSYIRARGLLLISANAQWDIDNKIEINIDSILSHIVDKKPFVSRMFIKSIPN
                     ITKYKKNLIRRIKMELSNADISIYNNNMKPLVEKDINDTLSNLS"
     gene            28158..29465
                     /locus_tag="PC523_001164"
     CDS             28158..29465
                     /locus_tag="PC523_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474708.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKAEIGCVEEIDSEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVITIDKRY
                     DYRKELKLELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYAILVEQSKVLTDEAFDCNLASRNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    29551..29817
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            29943..30746
                     /locus_tag="PC523_001165"
     CDS             29943..30746
                     /locus_tag="PC523_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2474709.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGNTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            30784..31395
                     /locus_tag="PC523_001166"
     CDS             30784..31395
                     /locus_tag="PC523_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2474710.1"
                     /translation="MLQGLEIVIAMFCITLIISIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRIIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            31426..32097
                     /locus_tag="PC523_001167"
     CDS             31426..32097
                     /locus_tag="PC523_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF2474711.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            32392..32754
                     /locus_tag="PC523_001168"
     CDS             32392..32754
                     /locus_tag="PC523_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDF2474712.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPNKEELKS
                     IYKTSGYDIKKFFNTSGVKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLFDGKN
                     VLLGFKEDVWKSTLLKED"
     gene            33189..33890
                     /locus_tag="PC523_001169"
     CDS             33189..33890
                     /locus_tag="PC523_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HDF2474713.1"
                     /translation="MSIEDLYDFECVPLNNEVDIDVLFEKMIEDGKEKEYTPVIVIDE
                     KVGLLRKNIDIIIKEYGSLENYREYCLTKYNEIDVNQFFDFRKREISELLKELVDLED
                     DFYDYKHFRPIMNIDIFHEYNKIYIAKVPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKDLIKDGKKFDELCIELVFYCEDIIIQGYETLNAL
                     KDVLKRSTILLFWWD"
     gene            34199..35017
                     /gene="larE"
                     /locus_tag="PC523_001170"
     CDS             34199..35017
                     /gene="larE"
                     /locus_tag="PC523_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HDF2474714.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVVAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGIDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSGLKKEE
                     IRSLSKILGLKTFNKPSFACLASRIPYGVEITDENLRIIEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            35072..36220
                     /locus_tag="PC523_001171"
     CDS             35072..36220
                     /locus_tag="PC523_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDF2474715.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            36356..37744
                     /locus_tag="PC523_001172"
     CDS             36356..37744
                     /locus_tag="PC523_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474716.1"
                     /translation="MKVLKLITVVAMMMLFVTGCNIKTESPEQLAKIPNYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGS
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGNTGNNRNTYSSKASANIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTSTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            37773..38471
                     /locus_tag="PC523_001173"
     CDS             37773..38471
                     /locus_tag="PC523_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2474717.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     ENDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALIRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            38474..39907
                     /locus_tag="PC523_001174"
     CDS             38474..39907
                     /locus_tag="PC523_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2474718.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVAFQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            39929..40666
                     /gene="truA"
                     /locus_tag="PC523_001175"
     CDS             39929..40666
                     /gene="truA"
                     /locus_tag="PC523_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDF2474719.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKAVEEVDERFHS
                     RYNVKSKTYMYRIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNIIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESRDRCQSSDTAPAKGLCLWKVRY"
     misc_binding    40777..40984
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            41237..42436
                     /locus_tag="PC523_001176"
     CDS             41237..42436
                     /locus_tag="PC523_001176"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDF2474720.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEIYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYETHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            42686..43732
                     /locus_tag="PC523_001177"
     CDS             42686..43732
                     /locus_tag="PC523_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474721.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNNFFFLLSGF
                     GSFCYMKTNKSELKRMVALGDGRTKKMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DSGHPVVLGALDMYYLPYYKKLYHIEHLPFHYVLMVGYDDEKVYLYDCGRTELLNLSY
                     DKLRESMNCSYPGLSKENTICTIRMNTSKNKYKIAKEVIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELSQWKTELEKDDYDKILTNMIMFFGTVPTIPNAIKGIKEPDTIQFKGGFDK
                     MSKMLKSIGEEYKNDSFIQVAEIFDKTSLIIEKISNIIVDYLTQKYDDTDQLPLLFSE
                     VLKDMKTGYLILNV"
     gene            43838..45001
                     /locus_tag="PC523_001178"
     CDS             43838..45001
                     /locus_tag="PC523_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2474722.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDDS
                     IKTLQQILFFKEIDIPLREVKEIMSSPYFDKLEALKNQRKLLILKRKRLDELIELINQ
                     TLRGEGNINFKEFDMSEYFDVLEEFKREHKSKIIKIYGSVEKYNEYIERVKFNEDKIA
                     KMAIKQYGTIEKFAQAIKTNFSSDILNLGEKFDRYKNDCLKEQHPKLKELYRKLAEDL
                     SKNPSSTELQEIAKEITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSKEIQSIVEEIDNEMKRSND
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGNGASKFIGEAFKIYFDNNNC"
     gene            complement(45090..45470)
                     /locus_tag="PC523_001179"
     CDS             complement(45090..45470)
                     /locus_tag="PC523_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HDF2474723.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKNNISVLEKSIE
                     NENWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EIESIKTYLGNIIESVKFDVNYIF"
     gene            complement(45473..46132)
                     /locus_tag="PC523_001180"
     CDS             complement(45473..46132)
                     /locus_tag="PC523_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDF2474724.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERVSIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            complement(46238..47212)
                     /locus_tag="PC523_001181"
     CDS             complement(46238..47212)
                     /locus_tag="PC523_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2474725.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGTELISIGGVLVDNEFNTI
                     DNYYSLVKPRYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDIIGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAMDLMNVCKCFFFNYPKNIDILEELYNKLPAFKTPLYYIPYFED
                     ENFELKFDRTPEDIIVYLKQILNILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKINEFEEYPDFILKLGDKKSFVESVIKINKSNRKSIKNLILKLSKKP"
     gene            complement(47252..47599)
                     /locus_tag="PC523_001182"
     CDS             complement(47252..47599)
                     /locus_tag="PC523_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474726.1"
                     /translation="MLNLKEIREEIIKKYIGDILISELTDELKSIIITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILLDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRIIISIDSYDD"
     gene            complement(47818..48774)
                     /locus_tag="PC523_001183"
     CDS             complement(47818..48774)
                     /locus_tag="PC523_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDF2474727.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRAKEGNPVC
                     ESGIFEILDVTDENRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EVARELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
BASE COUNT        19587 a         4915 c         8284 g        16208 t
ORIGIN      
        1 gagaacattt caacaagagt atagagatta tttagattta gctaagtttt tacaactaat
       61 gcctggaata ggagcatttg taggatttta tgtgaatgga aaattaattg ataaattatg
      121 tgaaactgct gtgtctgctt atagattgag gattcgagag cttaagttat aaaagttata
      181 agagaagtta taacataaaa aattagttat acaatatatc tatatacatt taattttgca
      241 ttgataaaaa caattatgga taaaaggtaa agcatgatag aatctaatgt agaggtttgt
      301 attttaatgg aaaaatttag tttttaatgt ttaagataca aattggatta tgaagagaag
      361 tgattgggag gaatattttc atgacaaatt tgccaaaaca atttgatagc tttaatgaag
      421 ctagacaaaa aggatttata aatgcaaagg aacttaagga aagtgggaaa aagatggttg
      481 gagttttctg tacatttact ccagttgaaa ttccaatggc agctggagca actgtagtag
      541 gtgtatgcgg tgttagtgaa gagccaatac ctgatgcaga aagagtgctt ccaagaaatc
      601 tttgtccttt aattaaatca agctatgggc atgcaataac agatacctgt ccatatttct
      661 atttttctga tttgttgata ggagaaacca cttgtgatgg taagaaaaaa atgtatgaag
      721 aacttgcaaa agttaagcca acatatgtaa tgcatttgcc aaatacctca aaaggagaat
      781 ttgcctataa actttggaaa gatgaaatga taagattaaa agaagaggtt gagaaatctt
      841 taggtgtaac tataactgaa gaagatataa gagctgctat aaaagataaa aatgaagaaa
      901 gagaactttt aaaagaattt tatgcacttg gtaaattaca accatctgca cttacaggtc
      961 ttgagttaca taatgtttta tatcaagcag gttttaaatt tgaccgtgca gaacttaaac
     1021 attctttaag aaaagttata gatgatatga aagaaagata tgaaaaagga gaatgtcctg
     1081 ttaaaaaaga taaaccaaga atattgataa caggttctcc tattggtgga ataagtgaaa
     1141 aaatagttaa aactttagaa gatgcagggg catcagtcgt tgcatatgaa ctttgtggag
     1201 ctattagatc aaatgattta ttggttgatg aagaaataga agatgtgtat gatgctttaa
     1261 cacaaaaata tataaatata ggttgttctt gtatgatgaa taatgataat cgtattgagt
     1321 tattagatag aataatagat gaatataatg tagatgctgt aatagatgta gttttacaag
     1381 catgtcatac atttaatata gaaagttata gaataagaga gtttgttaca aaagaaaaaa
     1441 acaagccatt tatgtctctt gaaactgatt attcaaaatc agatactgaa caattgagaa
     1501 ctagatttga agcatttgta gagatgttgt agaaatacat aaaaatagga ggaattatgt
     1561 ttagtatagg agtggactca gggtcagtgg caacaaaagg tgtattgttt gatggtgaaa
     1621 aaattattaa gaaaataatt ataccaactg gatggagccc taaaagtaca tctaaacaag
     1681 tttatgagtt gttaagtagt gaaattgata aaaaagatat aaaaaaagta gtaggtacag
     1741 gttatggaag aggagtaatg gattttgctg ataagaaagt aacagaaata acttgtcata
     1801 ctagaggagt ttacttttta aacaataacg taagaactat tttagatgta ggtggtcaag
     1861 atagtaaagt tataaattta gatagagatg gaaatgtatt taattttata atgaatgata
     1921 agtgtgctgc aggtactggg cgatttttag agattacttc taacctttta ggaagtgata
     1981 tagaaagtat tgatacattg gctcaaggac atgagccagt taatatatct agtatgtgta
     2041 cagtttttgc tgagtctgaa attgtaagtc ttctagctca aaatatttct gctggagagg
     2101 tagcagcagg gatacttaaa tctattgcaa ataaatccac ttcaatgcta gcaagaggtg
     2161 agataataga tgaagttgca tttacaggag ggcttgcaaa aagtaaggaa ttagtgaaaa
     2221 tgatagaaga aatactggat aaaaaaattt ttattgcaga agatactcaa ataataggag
     2281 ctttaggagc tgctgtaata gggtttagat aaaaatataa ataaaataaa atgagaaact
     2341 gagtttatcc taaataaatt cagtttttta ttttatttta tttatttata aaggaaaact
     2401 ttggtataat tgatttaaat gtttgtatat ttgtaaatac ttgatattta gtcatatata
     2461 acattagaat tttaaaaatt agctcaaaaa agggggagaa agcgtgaaaa gatttacttc
     2521 aaaaaaagta gcaaggtttg ttgcactgtt tttaatatct attttagtaa tgtcaacaat
     2581 tcctgcatca gctgacacta gcaatacatt gaatcagata aaaaaagcag aatacagtga
     2641 gacttataaa caatatctaa atgatgttaa aaatggacgt acagaaaaat ataatgggat
     2701 tataccaaat ccatatcaat tggaaggtac tcaaattaaa agtaaaggaa gaacagctcc
     2761 aacatcttat gaccctagaa aattaggtct tatgacatca gtaaaaaatc aagagaattt
     2821 aggaatatgt tgggattttg ctgcaatggc aactttagaa agcttcttga agttaaataa
     2881 ttatggagat tacgatttat cagaagaaca tttaagatgg tgggcttcag atggtgaata
     2941 tggatggtct gtaaatgata tggatggtgc tttaaattat gaagctatgg gatatttaac
     3001 atcttgctca ggtcctaaat tagaaaaaga tatcccgtat aatggtaaaa tatctaaagc
     3061 acaaggagca acaaaaccaa ctaatatgaa tacagcacct actgttttta atgtaacaga
     3121 tgctgtgtgt gtaagtaatg acattaattc tacaaaaaat gctatattac agtatggggc
     3181 agtaacatct gcctattatg aagatattaa atatcaaagt gatgaccaaa attcatatta
     3241 ttgtccaaca aaaagtttta atacaaatca tgctatatca attgtaggct gggatgataa
     3301 ctattctaaa agtaatttta attcaaatat tagaccatca aaaaatggtg cttggctaat
     3361 aaaaaatagc tggggagatt ataactctga aggtggatat ttctggatat cttatgaaga
     3421 caagacactt atgtctgata tagataattt ttcaatcaaa ggtggtcaaa aaccaagtga
     3481 tgatgagaaa atgtatcaac atgactatgc aagcattgtt cctttaatct caaataaaat
     3541 aacagcagca aatgtattcg attttaatag aggtgatgag actctaaaat ctgtaatgtt
     3601 tttaactgaa agtataggag ctaaatatga agtatattat gctccagtag taaatggtat
     3661 acctcaagaa aataatatga aaaaattaaa agaaggtact acacaatatt caggatatat
     3721 aacagttcca attgattctt ttgatatacc agaaggaaaa ggtgcaattg tagtcagtat
     3781 agaggggaaa aatggagaat caactatagg ctctgagtca aacgtgccag gatatgatac
     3841 atttaaagca aaagctaatt taggtgagag ttatattata gataatactg gaaagttctt
     3901 tgatataaac agagatagta aattctatcc atgtaatttt actataaaag ctgttacaga
     3961 aaagtcttct agtgagagtg taccaaatga gtctttaatt ggtagtgata gatatgaaac
     4021 tgctatcaaa gttagtcaaa atggtttcaa ttcttcagaa aatgtagttt tagtaaatgg
     4081 aggttcaata gtagatgcat tggcagcaac tccttttaca tctgctataa attcaccaat
     4141 attattgaca caaaaagaag ctttaaattc aaaaactaaa gtcgagatac agagattagg
     4201 cgctaagaaa gtatacctaa taggtggaga aaattctata agtaaagaaa ccgaacaaca
     4261 attaaaaagc ttaaatatat caacagagag aatatcagga agtgatagat acaagacaag
     4321 tttattgtta gctcaaaagt taaatagtat aaaaaatgta tctcaaatag cagttgtaaa
     4381 tggagtgaag ggattagctg atgcaattag tgtaggtgca gcagcagcag aaaataacat
     4441 accaatcata cttgcaaatg aaaagagtga attgcaagga gctgatgaat ttttaaattc
     4501 attaaatata gagaaatctt atattattgg aggaacagca tccctatcaa acaatctaga
     4561 aagtaagtta aaaaatccaa ctagattatc tggtagtagt agagatgaaa ctaattcaaa
     4621 gataatagat aatttttata aaaaagatac tttaaaaaat gtatttgtag ttaaaaatgg
     4681 aatcaagagt caaaatgatt taatagatgg attggcagtg ggtcctttag gagcaaaaac
     4741 tgggtcacca gtagttttag ttggtgataa attagctgat agccaaaaag aagttcttaa
     4801 gaataagact ttagaaaaag taactcaagt tggtggagga gcaaatcaaa atgcatttaa
     4861 agagttagta aaattaaaga gtgttaaata ggatacttag gtattggtat atattaatat
     4921 gtattagtaa attatactga taagagctga aaataattac tttaaattat tgataaataa
     4981 gataatcaat ggttgcaaag ttttgttttc agctttaaat ttatgtagta taagtttaaa
     5041 aacataaaaa agggttactt tatatagaaa atcttacatt ttatatataa ataacccata
     5101 tttaattaac tttttatagt gttctaatag atattattga acttaatggt aaatataatg
     5161 tgtaagtttc gtttaaagtt ggattagatt cgtctatata tcttgagcaa agtgtaattg
     5221 ataagctgtc tgcttctata atttcaccat atagaggaca actgctgaag taagaatgta
     5281 cttcaacgta tttatttaaa ttttcttgaa cataatcata aaaacggttc tttgtagtag
     5341 tagtcataaa atcaactcct ttatttttta ttaaagctga aacttttaaa aagataaata
     5401 tgattacttc tattagaaac taagttaaaa aataagcaaa tagatatgaa aactagaata
     5461 atatattatt tatatcttaa ttataacatt aaataataaa aaatgctata taaaattcag
     5521 aattttcgaa agtttttaag tctattttta aatatactag caaaactatt gacattaaat
     5581 agaaaaagta atatattgac tatataaaat attttagtca aactggagga cggaagatgc
     5641 ctaaatcata ttctgacaaa gaaagagagt atataataaa aagacttaaa gaagaagcaa
     5701 gattgtgtat ggagctatat ggtattagaa aaactactgt tgatgagctt gtaaaacgag
     5761 taaaaatacc aaaaggaact ttttatctat tctttcaatc aaaagagttg cttttttttg
     5821 aagtattgag agatatacat gactctatac agaaagaaat cttatatgag ataaatagac
     5881 tagataaaag cattacatgt gaacaattga cagatatatt tatgaaattc tataggatgg
     5941 ttgatagtac ttcaatctta aatctcatgg taaatggaga atttgagata ttagtgagaa
     6001 agttgccaga tgctataatt gaagaacatt ttagacatga tgattttgaa attggagaga
     6061 ttatatcttg cataccaaac gcaaaaaata aagatataga aagatttagt ggagcattta
     6121 gagcagtctt tcttaccatg ttatataagc gtgaggttgg gagtaattgt tttgaagatg
     6181 ctttaagatt aatgataaat ggattagtta tacaacttat ggaatagaat ttaaattttg
     6241 tttgctaata acttttatta aaaattatta acaaacaaaa aaattatttt ttattgacta
     6301 tatagctaaa atggtcaaaa ggttgaaagg gtgatattta atgattaaag ttgatgattt
     6361 atcatttagt tatacagata aaaatttttt acaaaatatt aattttgaag taggaaaagg
     6421 tgaaatacta ggttttttag gtccatcagg tgcaggaaaa tctacattac aaaagattct
     6481 aattggaatg ataactaatt ataaaggaag tgtcattgta aatggagtag agagtaaaag
     6541 gcactcaaat aaattttatg aaaatatagg agttgatttt gaatttccta gtttatatga
     6601 aaagttgact gcaatagaaa atttaaaata ttttgggtct ctatattcta aaaaattact
     6661 atctattgat aagttattaa aatcagtagg gctagaaaat aaagcaaata aaagggtatc
     6721 tgagtattca aaaggtatga aatctagatt aaactttata aaagctttat tacataatcc
     6781 agatatttta tttttagatg aaccaacaag tggtcttgac ccatcaaata gcaaggttat
     6841 gaaagatata attttatcag aaaaatctaa aggtaaaact attattttaa caactcataa
     6901 catgttagat gcaacagagc tttgtgacag agtagcattc atagtaaatg gtaaaatatc
     6961 tgctttagat acaccacata atcttattat gtcaaaagga gctattaaag ttaaatatac
     7021 ttactttgat aatggagaaa aaacttctga atgttttctg aataatactg ctaatgataa
     7081 aaatttgaat atgcttattg agaaaaataa acttttatca atacatagta gtgaaccaac
     7141 attaaatgat atttttattg agataacagg gaggaattta caatgaggct aaaaaatctt
     7201 gttttaggag atataaagtt tcaatttaaa tatggttttt attttttata tcttttttta
     7261 agtattatat atatttgtat tatcaatgta tttccaactt ttatgagaga aaaaatagct
     7321 ataattatga tttattctga cccagcagca atgggattgt tttttatggg agctattgta
     7381 cttcttgaga aaagtcaaag ggttttaaat tccttagctg tatctccagt aaaagtatct
     7441 gagtatattt tatcaaaggt aatttcatta ggagtaatat ctagtattgt agctatgctt
     7501 attgctatta cattaaattt agataatatt ataatatcaa ctatagggac ttttttttct
     7561 tctattatat tttctttgct tggattgata cttgcaagta aagcatctag tttgaatcag
     7621 tttattgttt taagtattcc tattgagatt atatgcttta ttccaccaat actcaatgta
     7681 cttgttgata caaaatcata tgcaaatttt tatcctttta atatatgtat ttctcttatt
     7741 agtggagata aaaattttat agtgattaat attttaattt taatatcaat gataataata
     7801 atttatttta taacttatta ttttatttgt agtagttgga aaaaggtagg tggagtaaaa
     7861 ctatgataaa gattataaat gcgtttaaac aagagtttga acaaattaaa agagacgcta
     7921 tgctttttat agtatgtgtc tctccaatat tatgtggagt atttataaaa tttggaatac
     7981 ccttaataca gaatatatca cttaacaagt tttattatca attaaattta gaaccgtatt
     8041 tattgatgtt tgatttactt ttagctttta ttacaccttt tatgttcttt tttgcttcta
     8101 caatggtcgt attgggagaa attgatgata gtatttcaag gtatttaata gtaactccgc
     8161 ttggcaaaac tggttatctt gtttcacgat ttggaatacc aggtatctta gcatttatta
     8221 ttactatgat tctacttatc tttttttctc taactagaat ttcttttttg ttaaacttat
     8281 caatttcttt attagtttta ttacaaagcg ttattatatc aatattagtg atttctttat
     8341 catcaaataa actagaagga atggtaatta cgaaattctc aggtgttttt atgatgggta
     8401 ttttagcacc attttttatt ttaaataaag tgcaatatat tctgttcttc ttaccaactt
     8461 tttggctatc aaaggcattt aaggaagata attatatcta tatgtttatt tccattatta
     8521 tatcattaat atggatttta ctactattta aaaagtttag taaaaaaata gttaaataag
     8581 acaatttaat atataaatgt aaggttgact tgacagtaaa attgaaaaat gttacattta
     8641 aaatgtaagg ttaacattac atgaatggag caaatatgaa aaataaaata aagatattaa
     8701 gagaaaaatt aggattgaca caagaacaac taggaagatt agttggaaca tctagacagg
     8761 ctataaatgc tatagaaact ggtaaaaatg aaccttcaat atggttggca tatgatattt
     8821 caagaatttt taatgaacca attgaaagtg tatttttatt tgaagaaagt gaaagaaaat
     8881 caagagcaca gataagtagg ggagaatatt atggctataa gaaagataag aacatttgat
     8941 gatgaaattt taagaaaaaa gagtaagtat gtagaaaacg tagataataa gataagagaa
     9001 atattaaatg acatggcaga gaccatgtat aatactccaa atggaggagg attagctgca
     9061 tgccaagtag gagtattaaa gcgactagtt gttatagatt taggagaagg acttattaag
     9121 cttgttaatc ctgaaattat taaacaagaa ggggaacaaa ttgttgttga aggatgttta
     9181 agttttcctg aagtatgggg taaattaaaa agaccaaaaa aagttacagt gcaagctcta
     9241 aacgaatacg gcgagaaaat agaaattaaa ggttcaggtt ttatggcaaa atgtctttgt
     9301 catgagatag accatttaaa tggtatagtt tttacagata aaattattga acatgtaaaa
     9361 ttgtaagata gataagatag attggttatt gaatttataa aatattttat agtaaggagt
     9421 taaattatga gattatggca taaagattta attgatgtat tacctaaaaa tcaattagta
     9481 agtcaatgga gagagctact tgctataaaa ggttctatag ataaaaaagg tactccaaac
     9541 catatattag tgaataaagt gttgaattat agtatagatg aatttaaatt ttatacaaaa
     9601 attgtacatg atgaaatgtt aaaaagaaat tataaaccta atgaacttaa atatacaggt
     9661 atattaaaat ggaaaaatag aaattttgca aatgatatat ctaatgaaca tagtttagat
     9721 ttagaaaatt tatatgatgg ttggcacaat aaaatgtatt taaagcaatg cttatataat
     9781 ttagaagaaa aagcaacatg tggaggaata cccataaatg aatggaatat attactttgt
     9841 aaatatagta aagattatga attgtggtat ggaaatatca tgttttaatt actatagatt
     9901 aacattataa tattattgta tataaagagg tgattatttg tataataaaa taaaatatat
     9961 tccaataaat tgtgaaatag cttgtaataa actaaaaagg agtatgccat ataaatggga
    10021 tttgaatatt tatcgtggat gtgagcatgg gtgtaaatac tgctatgcta tttactctca
    10081 taagtatatc aactccaata attattttga agaaatttat gtaaaaacaa atatagtgga
    10141 aaagcttgag agacaactta aaagcagtaa atggaagaaa gaagtaatca atataggagg
    10201 agttacagat agttatcaac caatagaagc tgactataaa attatgccag aaatattaaa
    10261 tttgctgata aagtacaaga caccagctat tatttctaca aaatcagatt taatcttaag
    10321 agattacgat ttaattgata agctatctag gattacatat atcaatgtag catctacaat
    10381 taccacagta aatgaagata cacaaaggct tattgagcca aatggtgtgg attcaattag
    10441 acgatttaaa atgttaaaag aatttagaaa aactaacgca tcagttggtc tacatattat
    10501 gccaattata ccatatatta cagatgattt tgataatata aatttgctat ttagacatgc
    10561 aaaagaatgt aatgtacatt atgttttacc tgggacttta tacttaaggg gtataactag
    10621 aggtattttt tttgactttg ttaaaaaaga gtttcctgaa ttatttgata aattatcaat
    10681 actatatagc acaggttcac ttaatagggg gtataaaaat caattgtata aaatggttaa
    10741 tgaacttaga tataaatatt ctctatcaag cagttactct aaaataatga aagaaaagtt
    10801 aaaaaactca ggagatgtac agatatcttt ttttgattag ttttatataa aaaaattttt
    10861 tcactatgat gattaaaatt tattaaaatt aaaatttatt gacaagtatt aaatttaaat
    10921 atacaattaa ttcataaaat aaaaatatga aaggatgatg ttgttcagtg atacttgata
    10981 ttataaaatc attgatataa gtattgctgg aaaagagtga ataaaatcaa atttaataag
    11041 caaacaaagt aaagctatag ttttattgtt gtgcttaaaa atatttgatt ctattttact
    11101 cttatttaaa ctcatctttt tgaagttaaa tcacagagta aactctgtga tttttttatt
    11161 ttaaaaataa attttaagga gtggtatgtt ttgttaaaca aattatgtat gtatttagaa
    11221 agaccagagc tttataaaca aagtgaaatt aatttttggg atgatgagta tatttcaaag
    11281 caattattaa aagcacactt agacactaat tttgaaggag caagtagaaa ttttaacttt
    11341 atcgaggatt ctgtaaattg gatagttaca gttgcaaatc cagttaagta tcctaaatta
    11401 cttgatttag gttgtggccc aggtttatat gcagagaaat ttgcacaaaa aggttataag
    11461 gtaactggaa ttgatttctc aaaacgttct attaactatg cacaaaatag aaataaggag
    11521 actaatttaa acataaatta cttgtttcaa agttatttaa atatgaatta taatgaggaa
    11581 tttgatttgg ctactttaat ttattgtgat tatggagctt tgtcaactga aaatagaagg
    11641 ttgttaatgg aaaaaattta tgatagttta aaacctggag gaaaattaat attagatgta
    11701 tttacaataa ataaatacaa taattttgag gaaattaaat attgggaaat aaatgaagat
    11761 ggaggatttt ggagtaatga aaaatatatg tgtctacaag ataattgtaa atacaatgat
    11821 tataatacat tagaacaaac tcttgtaata acagaaaaag atgaaaatgt gttttatgtt
    11881 tggaatcact atttttcaaa agaaagtttc ttattcgaaa ttgaaaatat tggatttaag
    11941 tctgttgagt tttttagtaa tgttaaaggt gaagcatatt ctgacgatag tatgactatg
    12001 ggattagttt tacaaaaata ataatttttt taaaatgaat aaataaaaac ttgaaacctc
    12061 taataataaa ataaaataag aaaagcttac atattgaaaa atggaaactt ttatgttatt
    12121 tttaatacat tacaaatttg cttatataaa aatcaacact taaattatta tttttatatg
    12181 aaatttatct atagtatatt tttataaaca agtaatgcta ttaaaattaa gtatatagtc
    12241 aaaagtgtat agcaaaaagg ctgttatatt aatgttagag gttcagggaa aatatataaa
    12301 gcaaagatat ttacagataa tgcaaatggt acaactataa gttagataat agaattatgt
    12361 aatcaagaat ttacaacaaa gtctaagata aggataatac caaattgcca tacaggtgct
    12421 agatgtgtaa atgaattaaa ttaatactat tgataaagta caacaagcac ataaactaat
    12481 tgatgagatt attaaaacta taagagatat agttgacata attaaaatat tgtaatctat
    12541 ttataatttt attataaatc aataatgatt aagattgaaa taatattaag gcatcaatgt
    12601 tataagttta caatataagg agtactatat agtattcctt acttacacat attaggcaat
    12661 aggaagtttt ttcatagtta agttttcaaa tattttttag ggaactttgt aattgagaat
    12721 taagggtata gtatgtgatg aacttactaa atttataatt aggttatata atggaataat
    12781 catcaatagt tttactttag ggaaaataca tgcttaagga agtatagctt actaatatta
    12841 ttaaaatgta tatttaaaat gataaaaaaa gaattagaca agtagttagt aaattgatta
    12901 tgcaaaattc tatcaagcag gaaccctata actttagtca tggaaggttc agtaggtaat
    12961 ctgttgaatt aagtttatat tatttaaata aactacttaa tttagataat acaaaaagtc
    13021 agaattttaa gaatagagct atataatctt agctattagg agaattacta tgataggaat
    13081 atattttagt ggaacaggaa attcaagata ttgtgtagaa aaatttttac aagaatatga
    13141 tataacagca aattcttatt ctattgaaga taatgaactt ttacagtgta ttaataatca
    13201 tgagaatatt atttttagtt atcctgtaca atatagcaat gtaccaaaaa tattgaaaga
    13261 tttcataata aatcattcaa ctttgtggag tggtaaacgt gtttttatta ttgcaactat
    13321 ggctctattt agtggggatg gctctggtgt gttatctaga atgttaaaaa aatatggtgc
    13381 tataactgtt ggtggattac atgtgaggat gccagatagt ataggagatg aaaaggtatt
    13441 aaaacattct ttagagtata atagaaagct tgtaataaaa gcagaaaaga aagtaactaa
    13501 ggctgctaaa aaattgaaat atggatcacc accaaaagaa gggttaggtt ttttgagcca
    13561 tattgtagga ttatttggac aacgattgta ttttatcaat aaaacaaacg tgtatacgga
    13621 caagataaaa attaatgctg aggaatgtat tgtatgtggt aactgtatta gtttatgtcc
    13681 aatgaagaat ctagtaatta aaaatgatat gattatttca aatggtcaat gtacaatgtg
    13741 ctatcgttgt gtaaataatt gtgctaaaaa agcaattaca ttgttaggta aaaaggttgt
    13801 tgaacagagt aatattaaaa aatacatgta agaactttaa aattttacta aatatacatt
    13861 gatgtatact ttatgttaga aagagagctc aaataatgga atactaccat aattgattca
    13921 tttgcaacta aattatttaa aggaaatact gctggaattt gtgttcttgg cagaagaata
    13981 cctttagagt taatacagaa aatagcagaa gaaaacaatt taccagaaac ggcatttgta
    14041 gttaaagata aaaggaatta tgaattatga tggtttactc ctaaagtaga gattgattta
    14101 tgtggtcatg caattttagc agctgcttat gtaatatcaa attttattga tgttaatgta
    14161 aaaaagatag atttccttac tcaaagtgga aagcttgaaa ttacacgaaa tgatatttcc
    14221 agaaataatg tttatcggaa tagagttatc accacaacaa actaatttga taggctgtgt
    14281 accaattgct gtttattctt caagagattt aattttattg ctaaacagtg aacaagaagt
    14341 tataaattat aaacctaatt acacacagct gagtaaattg acagactggt taggtataat
    14401 aatcacagct caaggaagta atacagattt tgtatcaaga tatttttgtc cagaattaga
    14461 ttcaggaaac cctgttactg gttcattgca ttgtaatctt attccatact ggtctgaaaa
    14521 ggtggaaaaa cgcaaaatgg tagctgctca actttctaat cgtggtggta taattcagtg
    14581 taaattatta agggataata ctgtaaaaat ttctggtaaa gtagtcttgt ttattcaggt
    14641 aacaataaag attgacattt aaagaagtat aataactaat ttataattta atttactatg
    14701 aattatgaaa aataattaat tttcttagag ttaattataa taaagtagag tgaaaaaatt
    14761 taatttcatt taaagcaata tgatagacgt tgatactaag attgacaagc atgattaaaa
    14821 aagatagtat ttaatctata aagataaaaa cacagtctgg ttggtagtcc agacgcagca
    14881 atagctgtaa gtaacctgcc tccttggttg tccattcttt agaaagtaat ttaaggagga
    14941 actagcaatg gacaaaatta gtggaaaatt aacagtctta tttgaagaac cattttggat
    15001 aggcatattt gaaagacaag atggtaaaaa atatgaagca tgtagggtag tttttggggc
    15061 agagcctaaa gaggtagaag tatatgaatt tatacttgaa agatttttct cattagattt
    15121 tggcagtatt aagttagaaa aaaatgtgac taaagacaaa attggatata aaagaatgca
    15181 gagaaaagta aaaaaggaac aagaaaaagg atctataggg actaaggctc aaaatgctct
    15241 taagcttcaa tatgaagaaa gaaaacagga tagaaaaaac ctagctaaag ctagaaaaga
    15301 agaagaaaaa gaaagacttt ttaatttgaa acaagaaaag agaaaagcta aacataaagg
    15361 tcattaattt taattggctt tctttttaat aattgcttga aaaggtgtat agaatcaaaa
    15421 actttataca ccttaattta ttgaaaaaat aaagctttat atttcaatta ctttaaaata
    15481 taaaaatata ctaataaaaa ttgtaaaaat attataaatg gtataccaaa tgtaaattta
    15541 atatgtttag tcttatgtct gaaagaatac attccaatca tagaacctat actgcctcca
    15601 attactgcta tatacataag agtagcttct tttattctcc atttgtgttt aattgctctt
    15661 tccttatcta tgtacattga aaagaaagca ataaaattaa taactataaa atatattaaa
    15721 ataaattttt tcatagtctt gcctccataa aaatatatta tacattataa aataagatat
    15781 ttgttttgga aagttaattt tgcaattagt acatataaaa tacttgataa gaattaatat
    15841 aatattaatt ggaaaattta aaaaaattaa acaattagca aatagggggt atatttataa
    15901 gagacaaagt gataaaattt acttagaaaa taatgtaaaa taacaggagg ataagacaaa
    15961 atgaaattcg tagtggaaaa agaagtcttt gataaattag aaaatgtatg ctttggtgta
    16021 gtagtagcaa aaggcataga taacactaaa gaaatcgaaa gaataagcaa tcttttagat
    16081 ataagtatag atagagtaga agattacttc aaagataaaa aagtaaagga atcagaagaa
    16141 attataccat atagagaggc atttagaagt ctaggtatga acccaaataa atttatgagt
    16201 tcaatagaag caatgacaac aagagttgct aagaataaga aacttcctca tataaatcct
    16261 atcgtagatt taggaaactc tatttcttta aaatatttac ttccaatggg tgctcatgat
    16321 atagatttta gaaatgatga tgtatatgtt agattttcta aaaaagggga taaatttgta
    16381 ccatttggag aaactgatgc tgagttaatg gaagaaggag aattaattta ttcagttgga
    16441 gacatggtaa aaactagaag atggatatgg agacaaggag aggaagggaa aattactaat
    16501 agctcaaaaa atatattttt tcctatagat ggttttactg atgctaattt agataaggta
    16561 atgagtgcaa gagaagaatt agcaaaattg ttaaaggaaa tatttaattg tgaaattaaa
    16621 gtaggttttg tagataaaga taatcctgaa atggaaatat aataagttgt tgaatatttg
    16681 ttaagatatt atatttactt aatgatgtta aaaaggtatt aaaaaagaaa ggtcgtagta
    16741 aatttgaaaa aaactataat aaagaatgta aatccaatga ttgaaaataa gtttatagga
    16801 ctttttgaaa tagagtataa aaacaaacta gatgaagata aggtttggat ggtagcatca
    16861 agaaaaagta acgagcaatt aaaaaatatt tatttgaaga atgaagaaga tagtgtagat
    16921 gcagttgtat tagctgggct tcataaaagt agtaaaaaat taattttaat aagacaattt
    16981 agagtaccta ttaatgatta tatatacgaa ttaccagcag gtcttgttga tgaaggagag
    17041 tctatagatg tttctgttga aagagaactt agagaagaaa cagggcttac tcttttagaa
    17101 attaaaaaaa ataaaagtat tgataaagtg tatctatctc ctggaatgag tgatgaatct
    17161 gttgcctttg tttattgtat atgtgatggg gatataacag ataaattttt agaagcagat
    17221 gaagagattg aggctctttt aatttctcaa gaagaggcaa aagagatttt acaaagtaat
    17281 cataagatag acacaaaagc atttttgatt ttgcaaatgt ttgtgagctt aggaactaaa
    17341 ttatttgaat agatatatgg aaaagaggtt atatagagaa gtaaaataat aaagtttatt
    17401 ttacttttct aaattataat ctcttttttt atacaataaa gtataatttt gtgtattttt
    17461 aaataagtga cttgactctt aaattttcat aatatgttat aaaccaattt tagatactgc
    17521 aataattaaa atccaaaaat tatttaacat atttgcaatt aaaatgagaa aaggtaaaga
    17581 ctgaatgatt gaaaaatttg atgtgaaata gctgattagt ttaatattat agtttgtagg
    17641 gttaaaaatt agaaattgta aaggagataa attctatgaa aaaattagtt attattttaa
    17701 ttagtatcat taatgtggtt agcttgacag cttgtaaaaa aagcaatgaa agtaaatata
    17761 ataatctaaa caacaaagaa tcaggacaaa caaccattga aatggaatta gataaaaatt
    17821 acgatacttc agactcattt gttaatgcac gtttgttttg tgtgtctaat gatatagatg
    17881 ttttagaaac agaaatttct tttcaaatgg atggagatag tggcattgta gaaataaaag
    17941 ataataaaac agatgaaact ttatggagta atacatggca tggaagagtt gataatgata
    18001 cattcacaat ttctcttgct aatatacaaa aggagaaaga gtatgctata tggtttactg
    18061 gaattaaaat aaatcatgca attgtaaagg ttagttttga aagcaatttg gttaaggaga
    18121 aagaaatacc ctcaaaataa attccagttt gtttataact taatattcag tacataaaag
    18181 cagttaatat ttatgattga ctgctttttc ttatttaaaa aggatgagct gtgcattttt
    18241 aattgaaata gttcatacac attaaaaatt ggtgaatata ttactggaga aggatggcaa
    18301 aatatgtccc aagggacatt gagcaaaata aatgacttca gcttctctgt gctaaaaagt
    18361 atatactaaa taagggttaa agtagataaa aatgatacac taattgtctt ttgataataa
    18421 aaaaacatga aatttaatta tttaatcaag aaaaagtatt gaataaatga tttatttagt
    18481 gtacaataat catgtaaaaa catagtaatg gagggagaca tttcataatg aaaattaagg
    18541 taggaatgaa cggatttgga agaataggaa gagctgtatt aagaatagct caagaagaat
    18601 taggagataa tatagaaatt gtagccataa atgcaagagc aacaacagag tctttggcac
    18661 atctatttac atatgattca tgctatggaa cttttagagg tgaagtagaa gcaaaggatg
    18721 aagacacact agtagtaaac tctaaagaaa taaaaatatt aagatataat gacccagaag
    18781 aattaccatg gaaagaattg ggagttgata tagtaataga atcaacagga ttgtttacac
    18841 aaagagaaaa ggcagaaaag catattaaag caggagcaaa aaaagtaatt ataacagccc
    18901 caggaaaaaa tgaagatata actatagtta tcggagtgaa tgaggaagaa tatgataatg
    18961 aaaagcacaa tataatttca aatgcatctt gtactacaaa ttgtctagct ccttttgcta
    19021 aagtattgga cgaaaaattt ggtatagtaa aagggttaat gactacagta cattcatata
    19081 ctaacgatca aagaatatta gataaaagcc ataaagattt gagaagagca agagcggcgg
    19141 ctgaatctat aatacctacc actacaggag cagcaaaagc tgtgtcaaga gtattaccac
    19201 aattagaagg aaaattaaat ggtttttcac ttagagtgcc aacaccaaca gtttcacttg
    19261 ttgatttagt atgcgaactt aaagaaaacg ttacatctga acaagttaac tctgtgttaa
    19321 aagatgctgc tgaaggagaa ttaaaaggtg ttttaggata ttgtgacaag ccacttgttt
    19381 ctatagatta taaaggagat tcaagatcat ctattataga cgctctatcc acaatggtta
    19441 tagaagacaa tatggttaag gtagtttctt ggtatgataa tgaatgggga tattcttcaa
    19501 gaactgttga tttagtaaaa tatgtagctg aaaaattaaa atagtatata attcttagaa
    19561 taaattaaaa aaaatttttt gaaaaaaatt gtataaaact aaaaaaaaga ttatgaaaaa
    19621 tattgaagga aaaatttttt cataatcttt tttctttcat gcaaaagata caagttacaa
    19681 cttatatttg tatattaggt cgttagagaa gtatatatat atttagtaag gaatcgtact
    19741 ttttataaaa tgaaagataa gatgtcgaaa acttgcataa atctgaaaaa tttttattat
    19801 taaaatactg attttcattt taaatgtgat aaaaaaatga caaataaagt tgcaaatagt
    19861 aatacttata agtataatta aaaaagagaa aaggttttgt taaactttat aacaaaagaa
    19921 aaagaaggga tgattcaaaa tggagaagtt aacagacagg agacaggtta aggcagtaag
    19981 catggaaacc ttcattttcc taatattact agtagttggt tttgggtatg ttggtagtat
    20041 tatgggtgca ggaatgatgt tcaaagtaat aatgagtacg gcacatgctt tactattaga
    20101 gacagttttc ttaattatgg ctatggcggt tcttgcaggc gcattaagcg cactactttc
    20161 agagtttgga gtaatagcgt taattaataa aattttcgct gtatttatga agcctttata
    20221 tgggctacct ggtgctagta tagcaggagc tataacaact tatttatcag ataatccagc
    20281 tattatacct tttgctaaag ataaaacatt cacacagtat tttaaacaat accaagttcc
    20341 agcattatgt aacttaggaa cagcctttgg tatgggactt atcttaacaa catttatgat
    20401 ttctcaaggt acagaatatg tacttccagc attaattggt aatttaggtg caattatagg
    20461 tagtgtcata agtgttagaa taatgttgac atttactaag aagttttata aatacaatcc
    20521 ggaagaagat aaagcaacag gaacattaga gaaaaaggat gaatttagag aaatcagaga
    20581 aggtaatgta tttcaaagag ctttagatgc aattcttgaa ggtggtaaaa tgggagtaga
    20641 tatgggaatg gctataatcc caggagtcct agtagtatgt acattggtaa tgttattaac
    20701 ttttggacca tctacagacc ctgttacagg taaagaagtt tatacaggag ctgcatatga
    20761 aggaataaaa ttacttccag ttattggaga caaacttgga tttatattag aaccattatt
    20821 tggatttaca tcaccagagg caatagcatt ccctataact tcattgggag ctgttggtgc
    20881 agcaatgtct ctagttccag agtttataaa gtcaggagct ataactccta atgatatagc
    20941 agtatttaca gctatgggta tgtgttggag tggatattta agtacacaca ttggtatgat
    21001 ggatgcatta aatgctagac agttggcagg taaagctatt ctttctcata caataggtgg
    21061 tttatgtgca ggagcagcag ctcactttat atttacactc atagtataaa tgtaatcagc
    21121 taaaataaaa aataatatac aaaaaaatgg ttgttgatat gtaataagaa tttgcatatc
    21181 aacaaccatt tttttctggc taaagataat ttataatacg taaacattaa gtggatatta
    21241 gagttaagtt atatttatgt aaaaagtaac aaaaatctgt agaaatttat gacaaaattt
    21301 agtacactaa ctatatgttc ttatttaaaa tttgaggtta gaattaggaa ttacctagta
    21361 gagtaagatt tgattaataa aagaatcata aaataaaaat attagtcata aacattataa
    21421 ataacttact taaaataaag ccatagaact tacaaaaatt aaaaaagcat ataaatatgg
    21481 aggggtacaa tggatgaatt atttcatata ggaattgatg taggttctac aacggtgaag
    21541 gttgtggtac ttaataatag taataatata gttcataaag aatatagaag acactattct
    21601 gatgtgaaaa aatcagttaa agaagtacta aatggtatat atgaaagatt tggaaacata
    21661 aatactacca ttataataac tggttcaggt ggaattggaa tatcaaaaaa attaggcgta
    21721 aaatttgttc aggaagttat atctagtaca aaatcaatag agtatttcca tcctgaaact
    21781 gatgttgtca tagaacttgg aggagaagat gcaaaaataa catatctaag tggaggaata
    21841 gaccaaagaa tgaatggaat atgtgcaggt ggcactggag cattcataga ccaaatggca
    21901 tcacttttaa agactgatgc cagtggatta aatgaacttg ctaaagggta taatgttata
    21961 tatcctatag cttcaagatg tggtgttttt gctaaaacag atattcaacc tcttataaat
    22021 gatggagcta agcaaactga tatagcaatg agtattttca atgcagttgt cgtacaaact
    22081 gtgagtgttc tctcttgtgg aagaaaaata gaaggaaatg ttgcgttttt aggtggtcca
    22141 ttatattttt tatctgaact tagagaagca tttaaaagag ttttggattt aaaagatgaa
    22201 aatataatct tcccagaaaa tgcacagctt tatattgcta ttggagcagg attattatca
    22261 gtagatgagt atagtataca actaaaatct ctaatagaaa aattggatag tataaagaat
    22321 attgaagatg gagaagtaaa tttattagaa cctttgttta aagacaaaaa tgagtatgaa
    22381 gaattttcaa gaagacatga aaaggaaaaa ataaattatg tggacataaa ttcaataaag
    22441 agtaattgtt atttgggaat tgatgcaggc tcaactacaa ctaaagcagc acttattgat
    22501 gaagatggaa gacttgtata ttcttattac aatagcaatg aaggaaatcc attaaaaaca
    22561 accattaaag tcataaatga tatatatgac atattaccaa aagatgttaa aatattgagt
    22621 tcaactgtaa ctggatacgg agaaggtctt attaaaaagg ctcttaaaat cgataatgga
    22681 gagatagaga ctatagcaca ttacaaagct gctaaatttt tcaataaaga tgtagatttt
    22741 atacttgata ttggtggtca agatatgaag tgcctaaaaa taaaagatgg agtaattgat
    22801 aatataatat taaatgaggc ttgttcatca ggttgtggct catttttaga gacatttgca
    22861 agttctttat ctatgacaat agaagaattt gcacatgaag gtatttattc tcaaaatcct
    22921 gtagatttag gttcaagatg tactgtattt atgaactcta gagtaaaaca agctcaaaaa
    22981 gaaggtgcaa gtgttggaga tatatcagcg gggctatctt attcagtaat aaaaaatgcc
    23041 ttatttaaag taataaagat tagagattta aatgagattg gtaataatat tgttgtacaa
    23101 ggtggaacct tttataatga tttagtgctt agaagctttg aaaaactaat tgggaaaaat
    23161 gtaattagac ctaatatttc aggaattatg ggagcttttg gttcagcact tatagcaaaa
    23221 gaaaaatatg aaagtggata caaaacatcc ttgttatcta aggaagagtt gaataatatc
    23281 aaattagaag caagtgtaac tagatgtaaa gggtgttcaa atcattgtct tcttacaata
    23341 aacaaatttt ctgataatga aatttttatt tcaggtaata gatgtgagaa aggtgaagct
    23401 atttatggcg ataaaaaaat agggttagaa aagaaaaaac caataaattt atttaagtac
    23461 aaatataata ggatttttag atacaaacca ttggaaaaag aagaagcaaa aaatggggaa
    23521 atagggatac caagagtatt aaatatgtat gaagattatc cattttggtt cacttttttc
    23581 aatgctatgg gctttagagt tgtactttca gataggtcat caaaacagtt atatgaaact
    23641 ggtataacta gtattgcatc tgaaacagtt tgttatccag gaaaattagt tcatggacat
    23701 atagaaaatt taattgcaaa gggaataaaa aatatatttt atccaagtgt aacaaatgaa
    23761 aataaagagg attcaaatgc agataattat tataattgtc ctgtagtaat atcatattct
    23821 gaagtaataa aaaataacgt agagaatata agaaataaga acataaacta tataaatcca
    23881 tttataagtc taaatgataa ggaaaaattg aagaaaagac tatatgatga gttatcaaga
    23941 catttttccg atataaaaat aactaaagaa gaaattaata atgcagttga taaagctact
    24001 gatgaacaga atttatttaa aatggagata caagcagcag gtgaaagagc acttaaagaa
    24061 ataaaagaaa gaaatatgaa gggtattgtg ttatgtggaa gaccatatca tatagaccca
    24121 gaaataaatc atggaatgcc agagcttata aattcattag atatggcagt gcttactgaa
    24181 gattctatat gtcacttagc caatatacaa agaccactta gagttgtaga ccaatgggta
    24241 tatcattcaa gattgtataa agcagccagt tttgtaagag ataaaaacta tttggaactt
    24301 gttcaactta attcatttgg ctgtgggtta gatgcagtaa ctacagacca ggttcaagaa
    24361 atacttaatg aaaaatcgaa gatatatact attataaaga ttgatgaagg caacaattta
    24421 ggagcagcta aaataaggat gaggtcttta aaagcagcta tgcttgagag agaaagcaaa
    24481 aacatagatg taaaaaatat aaaagtaaat aggatacagt atgctaaaaa taatcatata
    24541 acgaacaaac atactatatt agcaccacaa atgtctccaa tacattttca attccttgaa
    24601 aaagctgtta acttaagtgg ttataatata gaaattctaa aagatactga tagtagtgta
    24661 attgaagaag ggttaagata tgtaaataat gatgcatgtt atccagctat aatagtagta
    24721 ggtcagctaa taacagcatt aaaatctggc aaatatgatt taaataatac ttctgttact
    24781 ataactcaga ctggtggagg ttgtagagcc acaaattata ttggattctt aagaaaggct
    24841 atgtatgatg ctggatttaa agatgttcct gtcattgcac ttagtgtaaa tggaatagaa
    24901 gatagtggta taatggacaa tatatcacta aaattaatca atagactctt tatgtctgtt
    24961 gtttatggtg atttgctgat gaaagtacta tatagagtaa gaccatatga aaaagttact
    25021 ggaagtgcaa atgctctata tgaaaaatgg gttgatatat gcaaaacttc tcttgttaaa
    25081 gcaaaaattt ctgtatttac cagaaatata aaagatatag taaaagaatt tgataattta
    25141 gagatactag atatcaaaaa accaaaagta ggtttggttg gagaaatatt ggtgaaattc
    25201 catccaattg caaataataa tttagtagat atattagagc gagaaggtgc tgaagcagtc
    25261 gtaccagacc ttacaaattt cttcttaagt tgtgccttta atactatata caaacacact
    25321 catttagaag gaagtagaaa gagtagaatg ataggagaag tatttatata cataactgga
    25381 atatatcaaa gagtatataa aaaagcactt gataagagtg agagatttta tgctccagcc
    25441 aatataaaaa atgttgctaa aagtacagaa ccagtagtgt cattaggtaa tcaaaccgga
    25501 gaaggttggc ttttaacagg tgagatggtt gaattattaa atgaaggtgt agaaaacatt
    25561 atttgtatgc aaccttttgg atgtttacct aatcacataa ttggtaaggg ttctataaaa
    25621 gaattaaaga ggttgtataa aaatgcaaat ataataccta ttgattatga cccttctgca
    25681 agtgaggtaa atcaacttaa caggataaaa cttatgcttt ctaaggcttt taaaaatata
    25741 taagatttat attttaatat gttaaaagtt aatgaattta aaaatatact ctatattaag
    25801 tatctataaa aaagagttat cctatttttt agggtaactc tttattagtt aaaaaattat
    25861 atgtatactg atattaaagg ggggaagtaa tatgagttta gtatatgatg tttataaatc
    25921 aaaattagga aatttgtaca tattaagtga agatgatttt atagttagtg tatacatagg
    25981 agatgaaaaa tttaataatt taaaaaatga atatattaaa aaaggaattg atataaagag
    26041 aagtaaggaa gatttaaagg aagcaataag tcagatagat gattacttta atggaaaaag
    26101 aaaagtcttt aatttaaata ttcgtattga agggactgaa tttcaaaaat cagtttatag
    26161 ggaaatgtta aaaatacctt atggagaaac attgtgttat tcagatatag caagaaatat
    26221 aaataatcca aaatcagtaa gagcaatagg acaagcaagt aaaagtaata aaattccaat
    26281 aataatacct tgtcatagag tagttggcaa aaaaaatatt ggtggatata tggggaatca
    26341 ttctgatttg aaagagatac tattaaattt agaaagacag agttaactta gaaagaaatg
    26401 tttttattgt taatatttga tataataata ttaaaaataa gtatttttta caaaaacaat
    26461 aaagaattta ttttaattta caaaaatgag ggggaatttt aatgtgtgat aaagttatag
    26521 catcaaaata tcatgagaag ggatttaatt gtgcagaatc agttataaaa gcatataatg
    26581 aagaatttaa tacagatata ccagtttgcc taggtagtgg actgggttcg ggttgtggag
    26641 ttgctagttt atgtggtgca gtaaatgcat caaatataat aattggatac gtaaaaggca
    26701 gaaaccatga agatgaaagt actagagcta aagtatatgc taaagattta actacaacag
    26761 taagaaaaga gtatggttca gagttatgta tagacctaaa aaaagattta gttgcctgta
    26821 gagaaataat ggatttcgca tatgatagtc taaaggaaac tttaaaaaaa gaattataag
    26881 aaaataagct atcaagtaca aattatttga tagcttattt ttatagtcta tactaaataa
    26941 attaaacata ttttagaatt attatttgta aataaacatc aaataatttt aggtttttta
    27001 tttaatatag ctttttattt atatttgttt ttgaatgtgt tatactctgt ttaaataaat
    27061 atttgtaata agggagatga gaagattgga taatatcaat ttgcttatta atagattata
    27121 ttcaaaaaat cataatgaag catataaaac ttttttattc ttagaaaatg aaagcttaaa
    27181 atcaaatatt acatattgtt tttttgactc attcctagaa atgattaata acgaaaactc
    27241 atacattcga gcaagaggat tgttattaat ttccgcaaat gctcaatggg atattgataa
    27301 taaaattgaa ataaatatag atagcatact aagtcatatc gtagataaaa aaccttttgt
    27361 atcaagaatg tttattaaat ccatacctaa tattacaaag tataaaaaaa atctcattcg
    27421 tcgcataaaa atggaattaa gtaatgcaga tataagtatt tataataata atatgaaacc
    27481 attagtagaa aaggatataa acgatacatt atctaatctt agttaaatat taatatagaa
    27541 attggatatt atctagttgc aattatttat aagtaaaata ataaaacaat aatggtgcta
    27601 atttttatag ataaattaat tatgaaaaat ttatgtaagt aaaatatatt aacataaagg
    27661 taaaattttg tacactaaaa gggaatattt acagtttaat ggcagttttg aaataacaag
    27721 ttaaagctgt catttttatt tgaaaatttt tagaaaaagt acaatcgtgt taatctgtta
    27781 actagataag gtttatataa taatttggat gatatgatat aaaatatgtt gtatattaaa
    27841 tgttttcttt gacaatatat attcaaggat gataatatct atatgaataa aagctagcgt
    27901 atcaatttga taatatgtaa ttataacatt atgctataat tacaataaag tatgtaataa
    27961 tctgtagaaa ttacaagtta gttatttaat ttttcgaata actaaatcta aacggaatac
    28021 ataatgtact aaataaatga tgatactaaa atgatactat ttttatagaa atttattgag
    28081 gtatataatt aatgaaaata atatattaaa ttatataata tattgagttt tacataaaag
    28141 cgaaggtggg aataaaaatg aaagaaattg ctgatgctat aacatcagag tatttaagct
    28201 atggtatcaa tgaagatatt agtatctatg aaggtagatt ttgcatatat ttagataaaa
    28261 aatatagatg taatgggaaa atttattata aaatgacacc tccaatatct ataagtttta
    28321 aagcagagat aggttgtgtt gaagagatag acagtgaaga tgacaattta gctttggatt
    28381 atgataatgc tattcttgaa gttcatggat ataaaattat ttctataaca ataaatactt
    28441 taagtgaatt tagtgtagag ggttatatca atgatgactg cataaaatct aagaactcct
    28501 atgttgaata tgtagatttt aatataataa acttagataa aatacctgga aaactcatta
    28561 aatacaatga taaagtatac gcaggtagaa ttgaatttga tataaatgat tatgttataa
    28621 ctatagacaa gaggtatgat tatagaaaag aattaaaatt agaattaaaa tctaaatctg
    28681 gagcaataat aactcatata ggtagaatta gaagaaaaga tggtagaatt tttagaacaa
    28741 ataatacaat aaatctttta gatagaatat caacagcttt gagttttatg tgtggaagat
    28801 atgtagggtt ttgtttggcc aaagggtata gaagtggtaa tgaagtttat agaatatgga
    28861 atgaaaatca aatatctcct tttagatatg taccaacatg gtcagataca ctttcaaatt
    28921 atcataatat ggaaaaatat ataagtctta tgtgtaaaaa gctagaagat ttttattatg
    28981 gttccgctat aaagagtgtt gttgattggt atatagaatc tcttggaagt gctactatgg
    29041 aaaacaatat aatatctgtg caaattgcac ttgaaacttt gtcatatgca attttagtag
    29101 aacaaagtaa ggtacttaca gatgaagctt ttgactgtaa tttggcatct agaaatataa
    29161 gactcttact tgatacctgt aagataccat atggtaaaca tgaattaaat atatttgata
    29221 atataataaa aaataaattt gatgatggag tggatttagt tatttattta agaaatagca
    29281 tagttcatcc aagtagaaag actcatagag ctgtattaga agttgaggat atttggaata
    29341 taatttcaat aggaacgaga tatattgagc ttgttttact ttttatttta ggttatagag
    29401 gagaatattc aaatagatta gtagaaaggt gttatggtga agtggaagtt gtaccttgga
    29461 actagatttt tatatttatt ttaaaatata tattgacata tattacctta tgctataaaa
    29521 tcaatatcat aataacaatt cagaataaca ttaaatgcta agaaaagaaa tagtaaaagt
    29581 agttaatctt aaacagagag tttccgattg gtgagaggag caatagattt tactttgaac
    29641 acatctttga gcaggactct gaaagagtag aaatacttta ttaggagctt tccggaatcc
    29701 ttgcacccgt tatagtgcta gagtataatg atttgtttta ttgtacttaa agaggttagt
    29761 gtcgtgaggc attaatgaac agaggtggaa ccacgagagt taactcccgt cctctagcag
    29821 aagctagagg cgggagtttt ttaatataat tttttaagcg cgcagagaat tatattgcaa
    29881 gagatacact ttttaaaaaa taatcaaaat attttaaaaa ttaaaaggcg agggagaata
    29941 ttatgaaaaa tatattaaaa aaagtaggaa tatttacaat tatgttaggt ttattaggtg
    30001 gagttgtagg ttgttcaaag ccagataatg aaaaagataa ggatgcttca aaggaatcta
    30061 aaaaagaagt agttgtagga tttgataata catttgtacc aatgggattt ctagatgaaa
    30121 agggaaatac agtaggattt gatgtggatt tagctaaaga aacatttaag agacttggaa
    30181 tggaagttaa gtttcagcct atagattggt caatgaagga aactgaatta aatgattcaa
    30241 aaactgtaga tgtattatgg aatggatatt ctataacaga cgaaagaaag aaaatagtat
    30301 catatacaga accatattta caaaataagc agataatagt tactctaagt gattcaaaga
    30361 taaattcaaa agctgattta aaagataaag aagttggaac acaacaaggt tcaacagcac
    30421 ttgatgcagt ggaaaaggat aaagatttta tgaatagttt aaaaggtgga gcaccagttc
    30481 tttatgatac ttatgataaa gcacttagag atttagagat aggtagaact agtgcagttg
    30541 ttggtgatga agttttaatt agatactata tgggacaaaa aggtgaagac aaatacaaag
    30601 tgttaaaaga tgattttgga ttagaagatt atgttgtagc aacttctaaa gaaaatccag
    30661 aactttgtga gaaaataaat gaaacattaa aagaaatgaa aaaagatgga acttttgaca
    30721 aaatttatga taagtggttt aaatagaaga taagttagct gttaagaagg gggcactaaa
    30781 ggcatgttac aaggtttaga gatagttata gcaatgtttt gtataacatt gataatatca
    30841 attccattgg ggattggagt tgcattttta agattatcaa agaataaatt agtaagtggt
    30901 attacacaat gttatatatt gataatgaga ggtacacctt tacttttgca aatgatagtc
    30961 atattttacg gattacctct tttaggaatt gtatttgaca gatttacagc tggagtagta
    31021 gctttcttct taaattatgc agcatatttt gcagaaatat ttagaggtgg aatacagtca
    31081 atcgatagag gacaatatga agcttcaaaa gtactaggat ttgataaatt tactatgtat
    31141 aagagaataa tctttccaca ggtttttaaa agaatactag cacctatttc aaatgaagta
    31201 atcacattag taaaagatac atctttagta tatatattag gattaaatga tattttaaga
    31261 atatctcaaa tagcaatgaa cagggaagct agtctgttac cattatttga ggcaggagcg
    31321 atatatctta tatttgtagc tatacttact aagggatttg aattacttga aaaaaaatat
    31381 tcttattata gataaagtta caatattata ggaggaggtt tttatatgtt gaaaatcaaa
    31441 aacttaaata aatcatttaa aaaaaataga gtacttaaag atatttcttt tgagttagaa
    31501 gaaggtcaga ttggagtttt acttggtaag tctggtgcag gaaaaaccac aatattgaga
    31561 tgtataaatg gtcttgaaga atttgatagt ggagagatta taatagataa tgaggtaata
    31621 aaaaataaga gagatatggc aaaaatacga gggaaaatag gtatggtttt tcaaaatttc
    31681 aacttatttc ctcatatgac agtccttgaa aatataattg aatccccagt taatgttttt
    31741 aaagttccta gaaaagaggc tgaagagagg gctagagaac ttttaagatt agttgattta
    31801 gaagacaaat taaattctta tccatttgaa ttgtcaggag gacagcaaca aagagttgct
    31861 atagctaggt catgtgcatt gatgccaaaa gttctttgtt ttgatgaacc tacttctgca
    31921 cttgatattg atactattca aagagtagtc aatataatga acagacttaa agataaaggt
    31981 atgacaatat taataattac acatgatgtt gtcttttcaa ataatgttgc agataaaatt
    32041 ataagtataa aagatggtat agtagaaaat gttcaaataa aagaaaaaat tgtttagagt
    32101 tttataaata taaactaggg tagaatttta taatataaat aatgggcaag taaaatcaaa
    32161 tatactatgc ccttttgttg tctcaaaatt taatcttata ttttatgttt aagttacatt
    32221 taatttataa taaaaataaa aggactattt aaatatataa tgtaaataaa tgtttaattt
    32281 gaaacatgat aattttttat gttgaaagct tataatcatt atgatataaa ttggttatag
    32341 tatacaaatt atgggtataa caataaaaaa ttagaaagga atttttataa aatgattaaa
    32401 ttatatggat atacaaaatg ttctactgtt aaaaaagcaa aaaattggct taaggaaaat
    32461 aatttagaat ttgaagatat agatatggtt caaaatcctc ctaataaaga ggagttaaaa
    32521 tcaatttata agaccagtgg atatgatata aagaaatttt ttaatacaag tggtgtgaag
    32581 tatagagaat tgggtcttaa agatattgta aaaactgaat ctgatgataa acttttagaa
    32641 atattggtaa gtgatggtat gcttattaaa agaccattac tttttgatgg taaaaatgta
    32701 ttattaggct ttaaagaaga cgtatggaaa agtactttgc taaaagaaga ttagaaggat
    32761 ataggtgtgt tgattaaaat tattaattaa tacacctata aatttatgta ttaaatctaa
    32821 ttcttataaa tattaattag caaattcaaa tcaaaaaaat tcatatcaac aaacatatta
    32881 cttcttgtta caaatgatat catgtatttt atttaaaata gtataatatt acaacgaatt
    32941 gttacaaaat tacttattat taaccatgaa catttgtttt gtgctattat atttatataa
    33001 ggtaattgtc ataaaaaata tctttttcat acagatttat atagaaaaat gaaatgaata
    33061 tttgctttat ataagtatat tttgtaaatt gactctatat ttatagttta aataatagtg
    33121 gtattaaaaa tctgacaaca aaaataaaac tttcaaaaaa ttaaatctaa aaagaatagg
    33181 aaataaatat gagtattgaa gatttatatg attttgaatg tgttcctttg aacaatgagg
    33241 ttgatattga tgttttattt gaaaagatga tagaagatgg aaaagaaaaa gaatatacac
    33301 cagttatagt cattgacgaa aaagtagggc tattaagaaa aaatatcgat ataattatta
    33361 aagagtatgg ttctcttgaa aattatagag agtattgttt aacaaaatac aatgaaattg
    33421 atgtaaacca attttttgat tttaggaagc gtgagatttc agaattatta aaggagctag
    33481 ttgatttaga agatgatttt tatgattata aacattttag accaattatg aatatagata
    33541 tatttcatga atacaataaa atttacatag ctaaagttcc aacaattaaa ccatacgaag
    33601 tatttgcata catacctatt ggtggattta atgattgtcc tagagatgaa gaacacatag
    33661 ctattgcaaa atattggtat gaaaaacatg gaatctttcc aattgcaatt ggatgtgata
    33721 cagttcaata ttctgtaaaa gatttaatta aagatggtaa aaaatttgat gaattatgta
    33781 ttgaattagt attttattgt gaggatatta ttatacaagg gtatgaaacc ttaaatgctc
    33841 ttaaggatgt attaaaacgc tctacaatat tgcttttttg gtgggattga tataatcaat
    33901 taacatattg taacataggt ttgaggaagg agatataaaa tatcgttaga acttcgtgta
    33961 ctagagttac aaacttgtac ttcagttgta ggtaaatgat gagttcttta tgacttttaa
    34021 acatattact atgttacact aggtttttat acacagtgta gcgcagtaat atgtttaaat
    34081 ttataaagat ttaaaaaata ctttgatatt ataatttata aaataaatac tttatatgtt
    34141 ataatcatta agaatgtaaa aaataatata tagttttata gttcatgttt ttctagatat
    34201 ggaggtaaat tttgtgaagg aaaaagaaaa attagataaa ttaaaaaaaa tgttacttga
    34261 attaggaagt gttgtagttg cttattctgg tggagtagat agtaattttt tgcttaaggt
    34321 cgcaaaagat acattaggtg aaaatgttgt agctgtaact attcatgcaa tgatgcattc
    34381 aagtagagaa attgaagagg ccaaacaata tactcaaaat tttggtgtta aacatatcat
    34441 attaaatata gaaaactttg atttaaaaga atttaaagaa aatggtattg atagatgtta
    34501 tcattgtaaa aaatatatat tctctaaaat aaaagaggta gcaaaggaac ataatataaa
    34561 gtacatagta gatgggacaa atatagatga tttaggagat tatagacctg ggcttaaggc
    34621 actgagtgaa ttaggagtaa ttagtccatt aaaagatagt ggtttaaaaa aagaagaaat
    34681 taggtcactt tcaaaaatat taggattaaa gacttttaat aaaccatcat ttgcttgtct
    34741 tgcaagtaga atcccttatg gagttgagat aacagatgaa aatttaagaa taatagaaaa
    34801 gagtgaagaa tatttatcca atttaggttt ttctcaattt agagtgagaa tgcatggaga
    34861 tatagctaga atagaagttg gacaagaaga attaggtaaa ttttttgaaa ataacaactt
    34921 taataaagtt gatactaaat taaaaatatt tggctttaaa tatgttacac tagatatgtc
    34981 aggatataaa atgggaagta tgaatttaaa tgtgtagatt attttaaaaa taaataagtt
    35041 ttatagttaa gataaagaaa caggaggttt tatgaaaaat tatacattaa tatcgccatg
    35101 tttttttgga atggaaaaaa tgctggctag agagattact aatttaggat atgaaataat
    35161 aaaaacagaa gatggaagaa taacatataa aactgatgag tttggaatag caaagtcaaa
    35221 catgtggttg agatgtgctg aaagagtaca tcttaaaatt gctgaatttg aggcaaaaag
    35281 ttttgatgaa ttatttgaaa atacaaaaag aataaactgg tctagatata taccttatgg
    35341 agctcaattt cctatatcaa aagcttcctc tataaaatca aaattataca gtacaccaga
    35401 tgtacaagcc atagtaaaaa aggctatagt agaaagctta aagaaaagtt atttggaaga
    35461 tggcttgctt aaagaagata aggaaaaata ccctattttt gtatttatac acaaagataa
    35521 agtaactatt tctatagata caacaggaga tgctttgcat aagagaggat atagagaaaa
    35581 agcaaataaa gctccaataa gagagacttt agctgctggg cttatttatt taactccatg
    35641 gaaagcaggt agagttttag tagaccctat gtgtggttct ggtactatat tgattgaagc
    35701 tgctatgatt ggtataaata tggcacctgg tttaaataga gagtttatat cagaaaaatg
    35761 gagaactctt gataaaaaaa tttggtggga tgtaagaaaa gatgctttta ataagataga
    35821 taatgaatct aaatttaaga tttatggata tgatatagat gaagaatcta tagatatagc
    35881 tagagaaaat gctgaaatag caggtgtaga tgaatatata gagtttaatg taggagatgc
    35941 aacgcaattt aaaagtgaag atgagtttgg atttataatt actaatcctc cttatgggga
    36001 aagattggaa gataaagata gtgttaaaca gttgtataag gagcttggtt atgcatttag
    36061 aaaattaaaa aattggtcat attatttgat aacatcttat gaagattttg aatatgagtt
    36121 tgggcaaaaa gcagataaaa aaagaaagtt gtataatggt atgctaaaaa ctaacttttt
    36181 ccaatatcca ggtcctaagc ctcctagaaa taataaataa gttttacatg attcatttgt
    36241 aaaatataaa attttattaa aataagtaaa acattatgac gccttgaaac gtctaattat
    36301 tgtgaggaga tttttgtgaa caaatttaaa gatttatagg attaggagga gttaaatgaa
    36361 agtgttaaag cttattactg tagtagccat gatgatgtta tttgttactg ggtgtaatat
    36421 aaaaactgaa tctccagaac aattagcgaa gatacctaac tatgatgata caaaaaaagt
    36481 attgtatgat ggtattgacc agttattaaa accagattca tctatgatat tgccttctaa
    36541 tacaaaagaa gtaggaaaaa taaataaggt agatttaaac agcgatggaa ttgatgaatt
    36601 agtagtattt gaacaaaaag aagatttaag taataatgtg agtcaggtag ggtttgtaac
    36661 cttgagttac aatggagaaa aatacgtact tggagaccat ttcttagaaa atggcgaatc
    36721 aatagaatat gctaattttt atgatttaga ctcagatgga tataaggaag ttatcttatt
    36781 agttaaatcc aaagataaaa caaatatgca tatatataaa gtagtagata atgaaataac
    36841 aaaaatatat gacttggatg catcatggct tcaaaataaa gaagatttta atgatatgaa
    36901 agtaaaaatt ggatatatag atggagatga caagttagat atattgatgc ttcatttaaa
    36961 taataagact aatgaaatgt ttgcaagtgt tgcaaatttc gatgggagta tgaaaatcaa
    37021 agattctgtt aagtttgaaa atgtaaaaaa cctaagtgaa ctatatataa cattaggtaa
    37081 tgttgcttcg tctgttggaa attcttcggc agctataaaa ggtattgtac ttgatatccc
    37141 tatattgaaa gataataatt atattactca gatactttat atgaaagatg gaaaaattaa
    37201 caaagctttt agtgattatg ataaaactat tactaaatct tattatatac ctgtagatga
    37261 tattgataaa gataagatta tagaaatacc tatagttgca ggtaatacag gtaataatag
    37321 aaatacttat agttctaaag catctgcaaa tataagttgg tatagatgga atggaaaaga
    37381 aggtgctgat tcaagtttaa tatttacaag tcagatatat tataattata aatataattt
    37441 taaattattt attccaaata atttatttga taaaatactt gttgaacaag aatttgttgg
    37501 agaaaaggca gtatttaaat tttactattt tgactattca gaccgtaaga atttatttaa
    37561 tatagtagta gagagtaaaa acaaacttga agatagaaaa aatacaagta ctcaaaattc
    37621 tattgtactt caagagagcg atgaatatac tttcttacta gttgtaaaca atgcaaatga
    37681 aatggataag ctagatatga ctatagacgc attaaaagaa tatttctcat taatatatgg
    37741 ttaatattat actcaattgg aggttttatt ttatgaagga aaaaatactt atactagaag
    37801 atgaaattgg tattagaagt tttgttagta taaacttaaa aagagaaggc tatgagatag
    37861 ttgaggcagg tactggaaga gaagctatag aaaaaatgac aacagaaaat gatattacaa
    37921 tagctctttt agatgtgatg cttccagata taagtgggat agaggtatgt aaatttataa
    37981 gggagaattt tgaccaagta ggtataataa tgcttactgc taaagctcaa gaggatgata
    38041 aaatagaagg atttatttca ggtgcagatg attatattat aaaaccattt agcataaagg
    38101 agcttttagt tagagtttct gctcttatta gaagagtagc aaaagatgat tcaagtgtta
    38161 aatcaagtga aatagtatct ccaccattta tacttgatat agacaaaaga aaattattta
    38221 aaaatggaaa ggaaattgag cttacaccaa ctgaattttc aatagtgaaa tatctaatat
    38281 caaatgctaa gcaatcgtta agtagagacc aaatattaga tgaagtgtgg ggaactaatt
    38341 atttatatga ttttaaaata gtagatgtaa atataagaag gataagaaat aaaatagaag
    38401 atgacccatc aaaaccaaaa tatatacaaa ctatttgggg ttatggatac tgttttagaa
    38461 aggaagaata atagtgttac attttgaagg tctgagaaaa aaagtaataa aaaactattt
    38521 tataataatt attataatgg ttactctgtt tgaaggattg ttcatgtttt acatacaaaa
    38581 ctactattat gattctgtca aacaactact tgaatctgaa ataaaatatg ctgatgaata
    38641 taatgctatt acaatggaaa ctacaagttt tgagaaaaaa gtaaaaaata tatttgataa
    38701 acaaccttta actaaaaatt cagaatttgg aatttcaata atagataagg ataaaaatat
    38761 tatattagac caatatggct ttaaaagcaa agaaaaagca aactatgaag atgttaacaa
    38821 tgcattaaaa gacataaaaa ctaaaaattt aacaccatat acttatagaa taccagatac
    38881 aggggagcat gttatgagta tctccttacc acttaaagta aataatataa tagaaggtgt
    38941 tgttagatat acagtatcat tagatgctat tgataatgcg atattaaagc aagctacatg
    39001 gcttatatta gcaggaatat ttatacttat tatagctata ctaataagtc ttaaatttgc
    39061 tgagactctt ataaaaccat tacgagagtt aaagaaattt gcaaatgaat tggctgtagg
    39121 aaattataat ataaaattag aaaagatgaa gattgtagat gatgaaattg gtgatttagc
    39181 acagacattt gaacatatgg cacatgagat tgacaaaagt gaaaaattaa aagaagaatt
    39241 tatatcatct gtatctcatg aactaaggac tcctcttaca tccataaagg gatggagtga
    39301 aaccttaggc tatgaaagta taacaagaga agagttagac ttaggtttag gtattataca
    39361 agatgaaaca gaaagactca taaagctagt tgaagaatta ttagactttt caagactttc
    39421 ttcagataga ataaagcttc atgttgatat agttgatgtt gaaggtttaa tcgtaggtgt
    39481 tgtaaatcaa cttaaagtta aagcagccga aaaagatata tcacttttgt ttgaatttga
    39541 aaatgaattt atagagaaca tccaaggaga taaaaataga ttaagacagg ttctcataaa
    39601 tttaatacaa aattcattta aatttactag tcaaggtggt tacataaaag ttgtagcttt
    39661 tcaagatgaa gagataacaa ccatatcagt agaggataat ggttcaggta tagaaaagca
    39721 aaatttaaat aaagttttag ataaattttt ccaagaagat tataataagg ctggtagtgg
    39781 attaggacta gcaattagta atgaaatagt aaaacttcat ggtggaagaa tgaaaataga
    39841 aagtgaaaag aatgttggaa caaaaataac ttttaatata aaaaataagt ttgctaaaca
    39901 agcataaatt cttagaaaga ggattaagat gagaaacata aaaatgatag tagcatatga
    39961 tggttcaaga tataaaggat atcaaaaact aggtgacaat aatatgacta ttcaagaaaa
    40021 acttgaaaat gtcttaagta aaatgactaa tgaaacagtg gagataatcg gctctggaag
    40081 aactgatatg ggagcccatg caagaggaca agttgtaaat tttagaacta attgtatgga
    40141 ttctttggat aaaattcaaa aatatctata tgaatattta ccagaagata tagttgtaaa
    40201 agctgttgaa gaagttgatg agaggtttca ttcaagatac aacgtaaaat caaagactta
    40261 tatgtataga attgacaata ataagtatca caatcctttt ataagaaaat atgccactca
    40321 tgtaagtaaa aaattagatt tggatagaat gagaaaagca agtgagtatt tagtaggaga
    40381 gcatgatttt actagttttg cttcttctaa atctaagaaa aaatcaaatg taagagaaat
    40441 ttattctata aatataaaag aagatgataa tattatagaa atatatgtag aaggaaatgg
    40501 atttttgtat aacatggtaa gaataatagt aggtgctcta atagatgttg gtctaaaaag
    40561 aagagctcca caagatatta agtatatgct tgaatcaaga gatagatgcc aatcatcaga
    40621 cactgcacca gctaaaggac tatgtctttg gaaagtgaga tattagtcaa ttatataaag
    40681 ctttctttaa aataaaaata aaataaatta tatagttgac ataaatggaa aaataaacta
    40741 ttatatattt tataagagag atataaaact ttatatttaa atcctgtgag aaagagagta
    40801 acttctatgt gttttataga gagctgagga tggtggaagc ttagcaaata agtattagtg
    40861 aaaagaactt tggaggatac taattgaatt tagtatgtta gtaggtaatc tcgccttaag
    40921 agaaagagtg gataagttta tcttatcaat tagagtggta acgcggatat atttcgtctc
    40981 ttggtttttc caagaggcgt tttttaatat atttaattac tagtgtttat ttagtatgtt
    41041 gaacatttac taattgatta gctaagttta tcaataagag tggtaccgcg gatatttcgc
    41101 ctcttagtat tttaaaatac taaggggctt ttttagtatt aaaatgattt tatgtgaatc
    41161 taaattctca aagtcattca atattaagaa tttctataaa ataaaaatta taatttaaga
    41221 ttgggaggaa ttaaaaatga aggaaaaagt agtattagca tattcaggag gacttgacac
    41281 atcaatcata ataccttggt taaaggaaaa ttatgaagat atagatgtta tagctgtttg
    41341 tggaaatgta ggtcaggaag ataaaatgga agatgtgtat gaaaaagctt tacaaagtgg
    41401 agcatcaaag gcatatgtgg atgatataag tgaagaattt gtaactgaaa ctatatttaa
    41461 agctgtaaaa gcagaagcta aatatgaagg aaagtattta ttaggaactt ctttagcaag
    41521 acctataata gccaaaaaat tagttgaagt tgctcataaa gaaggtgcta aatatatatg
    41581 ccatggatgt acaggaaaag gtaatgacca agttagattt gaagctacaa ttgcagctct
    41641 tgacccgact ataaaagtta ttgcaccttg gagaatttgg gatataaaat caagagaaga
    41701 tgctatagac tatgctgaaa aacataatat aaaggtaaca gcaactaaag ctaaaatata
    41761 ttcagtagac gctaaccttt ggcatgtgtc aacagagggt ggagatatag agcatctaga
    41821 aaatgaacat aaaaaagatg tttacaaaca atgtgtagac ccagaagatg catgtgatgt
    41881 ggctgaatat gtagaaattt attttgaaaa aggtgtacct aaaaaggtaa atggagaaga
    41941 attatctcca gttgcactga ttcataaatt aaatgagtta ggttgtaaac atggaattgg
    42001 tgttatagat atagttgaga atagacttgt tggtatgaaa tcaagaggta tatatgagac
    42061 tccaggagga acaatccttt atgaaacaca taatatttta gaaagtgcta ctttagacaa
    42121 agatacatta catttcaaac aaatggtgtc ttataaatat ggagaactta tttacaatgg
    42181 attatggtat tgtaaattaa gagaatctat agatgcattt atggagcaaa ctcaagataa
    42241 tgttacagga acagtaaaag ttaaacttta caaaggtaac ataaaacctg ctggaatatt
    42301 tactgaaaat gctctttatg atgaaggaat atcttctttt ggaaatagtg aactttatga
    42361 ccacaaagat gcagaaggat ttattaattt attcacttta ccattaaaaa tcagagctat
    42421 gaaagctgga aaataataaa atagattatt caagagtatg taaaaacgat ttctaggttt
    42481 ttatatgctc ttttttattt ggaattatta aattatgcag taaacaatat aacaaaatat
    42541 attatagtat caaatcggtt attaatatga aaatataata cctaaaatat aaattttata
    42601 aatataatag ttatctattg actctctcat tgtaagaggg tttatactga atctagcaaa
    42661 aacttatagt gaggagatag attatatgag aaaaataatt gaaatcaaac atcattcaca
    42721 tgattatgaa tgtatgtgga atggtataga agatttatat ataaataaaa caggtgaatg
    42781 tcttccaaac aattttttct ttttgctatc aggttttggt tcgttttgtt atatgaaaac
    42841 aaataaatcg gagttaaaaa gaatggtagc tttgggagat ggaaggacaa aaaagatgta
    42901 taaattctta gcaccaattg tcggttttga atacaaacat catgagttta aaaaatatga
    42961 gcaagcatta aagaaagcaa aatctgaaat tgatagtgga catccagttg ttttaggagc
    43021 tttagatatg tattatttac catattataa aaagttatat catatagagc atcttccttt
    43081 tcactatgta ttaatggtag gatatgatga tgaaaaagta tatttgtatg attgtgggag
    43141 aacagaatta ttaaatcttt cttatgataa attacgtgaa agtatgaatt gcagttatcc
    43201 tggattaagc aaagaaaata ctatttgcac aataagaatg aacacttcaa aaaataaata
    43261 taaaattgct aaagaagtta ttgctataaa gcgtgatatg tttttaaatc cacctaaagg
    43321 atttttggga tataaaggtt ttgaaaagtt tattaaagag ttgtcacagt ggaaaactga
    43381 attggagaaa gatgattatg ataagatttt gaccaatatg attatgtttt ttggaacagt
    43441 accaacaatt ccaaatgcaa tcaaaggaat taaagaacca gacactattc aatttaaagg
    43501 tggatttgac aaaatgagta aaatgcttaa aagtattgga gaggaatata aaaatgactc
    43561 ttttatacaa gtagctgaga tttttgataa aacttctctt ataatcgaga aaatatccaa
    43621 tattatagta gattatttga ctcagaaata tgatgataca gaccaacttc cattactatt
    43681 ctctgaggtt ttaaaggata tgaaaacagg atatttaata ttgaatgtat aaaaatagat
    43741 tattgactct aacattacgt aatagtgtaa tatgaaaata tagcttgaat agattcgtaa
    43801 aggttgatag ctataaaatc aaatttgagg tgtagatatg agaacagtaa aacaagtttc
    43861 cgatttaact ggaataagtg taagagcatt acattattat gatgaaatag gtcttttaaa
    43921 accaaataaa attacagatg caggatatag gctttatgat gatgattcta ttaaaactct
    43981 tcaacaaatt ttatttttca aggagattga tattccttta agagaagtta aggagataat
    44041 gtcaagcccg tattttgata agttagaggc tttaaaaaat caaagaaaat tacttatttt
    44101 aaaacgaaaa agattggacg aattgataga acttataaat caaaccctaa ggggagaagg
    44161 aaatattaat tttaaagagt ttgatatgag cgagtatttt gatgttttag aagaatttaa
    44221 gagagagcat aagagtaaga taattaaaat ttatggtagt gtagaaaaat acaatgaata
    44281 tatagaaaga gttaaattca atgaagataa aattgctaaa atggctataa aacaatatgg
    44341 aactattgag aagtttgccc aagctataaa aacaaatttt agcagtgata tattgaattt
    44401 aggagaaaaa ttcgatagat acaaaaatga ttgtttaaaa gagcaacatc caaaattaaa
    44461 agaattatat agaaagcttg ctgaagattt aagcaaaaat ccctcttcaa cagaacttca
    44521 agaaatagca aaggagataa cagatatatc taaaaaagat tatgaaattt tctctatgga
    44581 tactggtgat gacaactggt attatatggt tcaaaattat ctggtaaatc ctatgtggat
    44641 agaagaagta gataaaaagt atggaagtgg agcaggcaag tttattggac aagtactaaa
    44701 aacttatcta agagatagaa aaccaaagat aaatacattg tatgaaaaac ttgtagaaga
    44761 tttgagtaga gattgttctt caaaggaaat tcaaagcatt gttgaagaaa tagacaatga
    44821 aatgaaaaga agtaatgact tttataaaat agacaatgga gaaagatact ttgattatat
    44881 gtcagagctt tatttacaag attctaacta cataaaagta actgacaaga attatggtaa
    44941 tggtgcatct aagtttattg gagaagcttt taagatttat tttgataata ataattgttg
    45001 aaaatttgga aataaaaaat gtgtaagctt cataaaagca taaaagtaag gatatagatt
    45061 agctccatct atatccttat ttaaagtcat taaaatatat aatttacatc aaacttgaca
    45121 ctttcaatga tatttcccag atatgttttt atactttcaa tttcttctaa agattttaat
    45181 ttattttcag tataaattgc tttgtccaga atattaaatc ttaatcctat ttcatcaaag
    45241 tactcatgat ttaaaacttt gtaccaatgg tttttttctt tattccaact tgtagatatt
    45301 gaatcagctt ctttttgagc tttttcccaa ttttcatttt ctatagattt ttctaaaaca
    45361 gatatattgt ttttatatcc ttctgtaaaa tcatatagtt tattgttaac gtaaaatcca
    45421 aataatacaa ataagatagt ccatagtata acaaatgttg tagatttcaa tattaatcac
    45481 cctttttatt ttgaataaat attttgtcat tttcatctaa aacacaaata agaacatctt
    45541 taaaagtttc tatgtgatta gattttaaaa tacccattaa ccagttagta tctttttgag
    45601 ctatgtctag atttttattt attattctac catccagtat aagtggaata ggtaaatgat
    45661 taaaagctct tggaggagta ctattgtagc ttgatgctgg tacaacactt agattaccat
    45721 cagtctccaa aatagcatac tgtacatctt ttaggttaaa gtatccctga atcctaagtt
    45781 gctccaataa ttcatctata ctaactcttt cttttttaag ctctttatat acaatttttc
    45841 ccttatctat taacacagaa ggtttacctg ataaaaatga acttaatttt ctgcttttta
    45901 atgataagaa tgaaattaga gtctgcataa ttacaagtcc agatattgct gcaacaccat
    45961 ttataattgg tatattatta ttttccattg ggacagatgc aacttctgca attaatagtg
    46021 ttataactaa atcaaaacag ttcatttcag ctatttcacc ttttcccatg actctaagtg
    46081 caattagcac tgttatgtat aatataatac ttcttattaa tactactgtc atatctatca
    46141 attactcctt taaaaaatat ttacacttag ttagtatgtc ctatataaaa attttaattc
    46201 aaagtataaa aaaccttaaa taattggatt caactactta aggttttttt gataatttta
    46261 atattaaatt ttttatagat tttctgttac ttttatttat tttaataaca gattcaacaa
    46321 aactcttttt atctcctaat tttaagataa aatcaggata ttcttcaaat tcattgattt
    46381 ttattaattt gaaagaagaa gatagatttt taaagttaat aattttagag ttattcacta
    46441 acacacttct ttttttaaaa taaatttctt ttttacttat atttagtata tttaatattt
    46501 gtttaagata tactattata tcttctggag ttctatcaaa tttgagttca aaattttcat
    46561 cttcaaaata ggggatataa tataagggtg ttttgaaagc aggtaattta ttatataatt
    46621 cttcaagaat atcgatattt ttagggtagt taaagaaaaa gcatttacat acattcatta
    46681 aatccatagc atccgaaagt gagttatgtt taatatctcc ttctacagaa aaaatttttt
    46741 tcatattcag taaagatatt tgcttagata agattctatt attatatgta atatgttgag
    46801 atataaaagg ttgaatatca atcatcatat taaataactc taagcactta cctgtatatt
    46861 tatatactct aaatgaagtt aaaagaccaa ttatatcaaa atcaccccag ttatatattg
    46921 taacatcatc aaatttacaa aaccatttat aaaaatcatc cataacatat aaaagattag
    46981 gagcattatt gatatctagt tggttcaatt tggttaagtt tttacattta tttgaaagta
    47041 ttttattgta tcttggctta actaaagagt aataattatc tattgtatta aattcattat
    47101 caactaaaac tccaccaata gaaattaact cagtgccatt atattctttt ggtttatttt
    47161 tattatcatc tagtatatta aattcaaagt ctataaataa cttgtatttc ataaatctaa
    47221 agcacatcct caggtaaatt atttattgag cctaatcatc ataagaatca atagatataa
    47281 tgattctaaa catcaaaggt tcattgcaac ttttatttat ttcataattt aattcaaata
    47341 aatcttttaa gatataattt ataagtattc tttcccaacc catttttaaa ttaaccttag
    47401 agtctagtaa tatattttct atttttttaa atatttctat atttgctggt attatactga
    47461 gctcaatatt acttttaaac gggttagact gtaaatctgt tataattata ctttttaatt
    47521 catctgtaag ttcagaaatt aaaatatcac ctatgtattt ttttattatt tcctctctaa
    47581 tttcttttaa atttagcaat attagtacct gctacttaaa cagtagcttt tcctttcttt
    47641 ttaataatat tatttatttt tgaatcttat ttggatatat tttaagatta aaagttttca
    47701 cctatatcat tttttatcta atttaaactt atattaacat attccaggct tatatctcca
    47761 ccctgcttat gttaaaggtg gagtatgttg cctattttga tgaaaaatga taggttacta
    47821 tttacctatt cctttttctg ataatttttc taacatatct ttggtcattt tatctaaatc
    47881 ataactaaac ttaaagttcc attcttttac tgcactacta ctgtctagag aattaggcca
    47941 agaatcagct atactttgtc ttacagggtc tacatcatac tctattacaa aatcaggaat
    48001 atattttttt atagaatcag ctatttcttc aggagaaaag ctcatagcag taatattaaa
    48061 agcatttcta tggataagtt tatccgctgg agcttcgatt aaatccacta ttgattgaag
    48121 tgcatcaggc atatacatca tatccatttt tgtaccttct gctatgtaag atttatatct
    48181 cttgtttttt aatgcttcat aatatatatc aactgcataa tctgtagttc ctccaccagg
    48241 aggcgtaaca taagaaataa gacctggaaa tctaacacct ctagtgtcta ctccaaattt
    48301 tgaatgataa taatcacata gtaattctcc agaaacttta gttacaccat acatagtatt
    48361 aggtctttgt aatgtgtctt gaggcgtcat attttttggt gaattttcac caaaggcagc
    48421 aattgaacta ggtgtaaaaa atttacaatc caattctcta gctacttcaa gaccatttat
    48481 taatccattc atatttaagt tccaagcttc tagaggttta ctttcagcaa ctgcagaaag
    48541 taaagaagct aggtgaatta tagtatctac atcatatttt tttgcgattt caaaaaaacg
    48601 attctcatct gtaacatcta atatttcaaa tataccagat tcacatactg gattaccttc
    48661 ttttgctctc cttgaactag caattacatt ttgttctcca tactcatttc taagctttat
    48721 tgttaattct gaacctattt gtcctaatgc acctgttata agtatttttt tcaattttat
    48781 aacctcctaa gggtttaatt tatgtagtac tacttatttt tattttagca aatcttttgt
    48841 ttaaatacaa agaattaaag ataataatat atggtctaat tgaattaagt gcagtaaagg
    48901 tctaaaaatt aaaagttaat atataatggt ctattgtata acaataaaag actagattaa
    48961 attattatat atagattatg tgactaagtc acag
//
LOCUS       DAMJVM010000019        48923 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.019, whole genome shotgun sequence.
ACCESSION   DAMJVM010000019 DAMJVM010000000
VERSION     DAMJVM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 48923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48923
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.019"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(234..1589)
                     /locus_tag="PC523_001184"
     CDS             complement(234..1589)
                     /locus_tag="PC523_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HDF2474728.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDESDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VREMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLNVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(1615..1884)
                     /locus_tag="PC523_001185"
     CDS             complement(1615..1884)
                     /locus_tag="PC523_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2474729.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            complement(2174..4135)
                     /locus_tag="PC523_001186"
     CDS             complement(2174..4135)
                     /locus_tag="PC523_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474730.1"
                     /translation="MKKYEIFIFISIVVLCFISFPYKVFGQEENCLEDFERWVQENKN
                     NKEKIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHVENGGSISIGINNIIATDGTALYVEEGGDLYIKSSYESVEKIKANGKDAI
                     GIYTENDIRIINKDIEVTGERAIGVYSKGDVEIEESSIKAYSNNNENELLRDNNKLVQ
                     SIISESKKVYIIDSYNELIPSIAEDSGYNIVKCCYRGIGVFSEEIKVSKDDKIENIKF
                     PQYMVLETSSGNQLDIDVEWDFTDYYEKLEKGEKFNITGKFKTETLNKEKIIINDDVV
                     PILNIYVMDKKPIDNLELEFQNTKKGYVAALFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRIRVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEQEND
                     DIDGDRGGGGRDDPDRDKDDNSQNGNNGNDNNDSNNNQNNNNENDNSDEKQPNAPKDD
                     VESSTGSNENKNPQGDIPKEGNIPKDGNKENNSDKEKGVFNKSSIIENDGDYDLNLKS
                     IFGINEHSFPYRESISNKNQDTNKNKSNISDYNGIINNNYKLESKEYNLKKDIIFLAC
                     ILTPIMIGSILIVNSDTRKSIIKFIKEKK"
     gene            complement(4318..5793)
                     /gene="spoIVA"
                     /locus_tag="PC523_001187"
     CDS             complement(4318..5793)
                     /gene="spoIVA"
                     /locus_tag="PC523_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HDF2474731.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIMQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(5883..6059)
                     /locus_tag="PC523_001188"
     CDS             complement(5883..6059)
                     /locus_tag="PC523_001188"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474732.1"
                     /translation="MYIKFFNCICSGIDRTAQLHLDSNTRILQRGDIVIHSRQVWYEN
                     YRAENERISICKRN"
     gene            complement(6072..6254)
                     /locus_tag="PC523_001189"
     CDS             complement(6072..6254)
                     /locus_tag="PC523_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I V"
                     /protein_id="HDF2474733.1"
                     /translation="MFCYINPKGTTVGIEKHIVSASFSEYMTDKSFVIKDVGSKIFEM
                     RYVKDVGDNALLKYVS"
     gene            6264..6536
                     /locus_tag="PC523_001190"
     CDS             6264..6536
                     /locus_tag="PC523_001190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474734.1"
                     /translation="MFPEDLGVHDSDISLMITGESIKERNKILLNNLVDTGYIPAKDL
                     DIINDIIISFYQNLLFLKKNTGKNSDDDELTMKTISSIEYIIKHAK"
     gene            complement(6692..7711)
                     /locus_tag="PC523_001191"
     CDS             complement(6692..7711)
                     /locus_tag="PC523_001191"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDF2474735.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIILAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTWLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(7742..8356)
                     /gene="plsY"
                     /locus_tag="PC523_001192"
     CDS             complement(7742..8356)
                     /gene="plsY"
                     /locus_tag="PC523_001192"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDF2474736.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVVITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(8390..9709)
                     /gene="der"
                     /locus_tag="PC523_001193"
     CDS             complement(8390..9709)
                     /gene="der"
                     /locus_tag="PC523_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDF2474737.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKELTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(9743..11071)
                     /locus_tag="PC523_001194"
     CDS             complement(9743..11071)
                     /locus_tag="PC523_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HDF2474738.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEIGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLEVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNGKSASKTIEQIHQMQQKYLKQLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPEDILKRDKKVSIATG
                     HSAYEFIQSMANAMMDKFKNLQINVYEIKNKFFGETITVSGLLTAKDLKEQLENKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMKIKIIPCLNEGKDFVDKILK"
     gene            complement(11307..11507)
                     /locus_tag="PC523_001195"
     CDS             complement(11307..11507)
                     /locus_tag="PC523_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474739.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(11509..12441)
                     /locus_tag="PC523_001196"
     CDS             complement(11509..12441)
                     /locus_tag="PC523_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HDF2474740.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLISQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLLLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(12487..13419)
                     /locus_tag="PC523_001197"
     CDS             complement(12487..13419)
                     /locus_tag="PC523_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HDF2474741.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYDLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNDVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKRTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(13474..14763)
                     /locus_tag="PC523_001198"
     CDS             complement(13474..14763)
                     /locus_tag="PC523_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2474742.1"
                     /translation="MEDLNSVYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLKSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILRFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(14792..15484)
                     /locus_tag="PC523_001199"
     CDS             complement(14792..15484)
                     /locus_tag="PC523_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2474743.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(15499..16260)
                     /gene="pgeF"
                     /locus_tag="PC523_001200"
     CDS             complement(15499..16260)
                     /gene="pgeF"
                     /locus_tag="PC523_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDF2474744.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCKEIDEKLKSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLICSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(16257..16718)
                     /gene="nrdR"
                     /locus_tag="PC523_001201"
     CDS             complement(16257..16718)
                     /gene="nrdR"
                     /locus_tag="PC523_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDF2474745.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIEEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKDLDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(16740..17003)
                     /locus_tag="PC523_001202"
     CDS             complement(16740..17003)
                     /locus_tag="PC523_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HDF2474746.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(17107..17880)
                     /gene="sigG"
                     /locus_tag="PC523_001203"
     CDS             complement(17107..17880)
                     /gene="sigG"
                     /locus_tag="PC523_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HDF2474747.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(17972..18694)
                     /gene="sigE"
                     /locus_tag="PC523_001204"
     CDS             complement(17972..18694)
                     /gene="sigE"
                     /locus_tag="PC523_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HDF2474748.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(18719..19591)
                     /locus_tag="PC523_001205"
     CDS             complement(18719..19591)
                     /locus_tag="PC523_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HDF2474749.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTIPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     KLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(19890..21164)
                     /locus_tag="PC523_001206"
     CDS             complement(19890..21164)
                     /locus_tag="PC523_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2474750.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGGIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEVPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(21449..22606)
                     /gene="ftsZ"
                     /locus_tag="PC523_001207"
     CDS             complement(21449..22606)
                     /gene="ftsZ"
                     /locus_tag="PC523_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDF2474751.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLETKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(22796..23149)
                     /locus_tag="PC523_001208"
     CDS             complement(22796..23149)
                     /locus_tag="PC523_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HDF2474752.1"
                     /translation="MLWVLGGLAFGVVIGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGISLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(23248..23922)
                     /locus_tag="PC523_001209"
     CDS             complement(23248..23922)
                     /locus_tag="PC523_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2474753.1"
                     /translation="MKKKIVILGAFILGIFISTYIKSLDPNRVYITLGQTENIESEVE
                     NTKKEIKNLKETLKNNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSSVTGPG
                     ITITMKDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFTK"
     gene            complement(23927..24625)
                     /locus_tag="PC523_001210"
     CDS             complement(23927..24625)
                     /locus_tag="PC523_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2474754.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEISSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(24648..25388)
                     /locus_tag="PC523_001211"
     CDS             complement(24648..25388)
                     /locus_tag="PC523_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HDF2474755.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNINLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSVDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(25649..26875)
                     /gene="murG"
                     /locus_tag="PC523_001212"
     CDS             complement(25649..26875)
                     /gene="murG"
                     /locus_tag="PC523_001212"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDF2474756.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDVVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEEKL
                     VFTGNPVRKEILLSRKNIARKNLSISEEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMESISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(26893..28023)
                     /gene="spoVE"
                     /locus_tag="PC523_001213"
     CDS             complement(26893..28023)
                     /gene="spoVE"
                     /locus_tag="PC523_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HDF2474757.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIERNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGVALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(28075..29430)
                     /locus_tag="PC523_001214"
     CDS             complement(28075..29430)
                     /locus_tag="PC523_001214"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDF2474758.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDIDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSNEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKTNIFMNQTSEDFCV
                     LNYDDEIVKGLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(29449..30417)
                     /locus_tag="PC523_001215"
     CDS             complement(29449..30417)
                     /locus_tag="PC523_001215"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDF2474759.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIVTVVLAWISIIAVF"
     gene            complement(30460..31833)
                     /locus_tag="PC523_001216"
     CDS             complement(30460..31833)
                     /locus_tag="PC523_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDF2474760.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGNISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLSKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     ANTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDIYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(32000..33979)
                     /locus_tag="PC523_001217"
     CDS             complement(32000..33979)
                     /locus_tag="PC523_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HDF2474761.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASKIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGIEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDVKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(33991..34338)
                     /locus_tag="PC523_001218"
     CDS             complement(33991..34338)
                     /locus_tag="PC523_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474762.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGTKCILLIATFAVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(34815..35750)
                     /gene="rsmH"
                     /locus_tag="PC523_001219"
     CDS             complement(34815..35750)
                     /gene="rsmH"
                     /locus_tag="PC523_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDF2474763.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAINTAKERLSEYEGRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKTIETTGELVDIIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKYLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEVEANPRSRSAKLRVAEKI"
     gene            complement(35780..36526)
                     /locus_tag="PC523_001220"
     CDS             complement(35780..36526)
                     /locus_tag="PC523_001220"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDF2474764.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKESKRVGIKEDD
                     VLNIAIIAIPAGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGLIAHIYLSKKNKNNISEE"
     gene            complement(36925..38211)
                     /locus_tag="PC523_001221"
     CDS             complement(36925..38211)
                     /locus_tag="PC523_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2474765.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPLGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPKSVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINDNFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKKIILDYIEKSKKEGLSKEEFDRTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(38226..39491)
                     /locus_tag="PC523_001222"
     CDS             complement(38226..39491)
                     /locus_tag="PC523_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2474766.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSISEFGYEDEIDSITSKELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIIDIPRADFIKNVDEVKVVDEQMEITQGKLVMGYRANVDYADTDRYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSILFISSGIETKDYDKAVEL
                     IKEQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFAFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            39807..40904
                     /gene="ychF"
                     /locus_tag="PC523_001223"
     CDS             39807..40904
                     /gene="ychF"
                     /locus_tag="PC523_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDF2474767.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQIFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
     gene            complement(41514..43793)
                     /locus_tag="PC523_001224"
     CDS             complement(41514..43793)
                     /locus_tag="PC523_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2474768.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEEYFEVDNILSILKKQAINSSFKRM
                     GVILPNGTAYTTDGYREDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSDINVYSV
                     PIYKNNDVKGVIFATHSTKLYEKILSVPTFNGKGYSYVARSNGDIVLNPSSKNANSTK
                     ENLFSYIGKSDSISKSSINNMKSNIKNFKSGSLSYSMSGRGYYLSYAPIGINDWYLFS
                     EVPKAAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRASIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTAVKRKYLKVYEIYAENLKEILIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGIFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPDFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRLIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSVKGKEIN"
     gene            complement(44062..45516)
                     /locus_tag="PC523_001225"
     CDS             complement(44062..45516)
                     /locus_tag="PC523_001225"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDF2474769.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVEIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTNADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAISEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            45680..46549
                     /locus_tag="PC523_001226"
     CDS             45680..46549
                     /locus_tag="PC523_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2474770.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKNIQFEYHNHDFNKIIIFINGNVT
                     YFVEGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDATSDLEK
                     CFKISTENKCNRLRLNMHDIESIKSFIEQINISESNTEFGNEILRNALFIQLMIFLNR
                     IFLKNENIESTEDVFYDETIKKVLDYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NSKNPLDITLLDE"
     gene            complement(46763..48835)
                     /locus_tag="PC523_001227"
     CDS             complement(46763..48835)
                     /locus_tag="PC523_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2474771.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMIDNSMYASIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAVMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDDSETNVEVIDMSKNELASEILVALGNKENLKSLDACITR
                     LRLIVNDINKVDKEKLKSLGAAGVMVIGNNVQVIFGPKSDSIKSDIKDIIEGKTKIES
                     AVIKNNDKNKMKKNMEVANMENALVENIYSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSNDGLIYSPVDGTIGVIFPTKHAIIIKSKKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGITTAKEENRVIIL
                     RDDIEKIG"
BASE COUNT        15324 a         8952 c         5054 g        19593 t
ORIGIN      
        1 tttttattaa ataacataac tttttagata tataaaaaac tttatattaa ttagatatta
       61 ttaaaataaa agcgatacat gaccttttaa atgtccatgt atcgctttat ttttgccaca
      121 atcttaacaa ctaattttta aaatttatta tcttaactat aatatttaaa tacatatact
      181 tatttaattt aaccatataa atatttaatt aaatattatc atatgtaata tatctaattt
      241 ttaaatgaat gtataggggc tggaatcctt cctcctcttt gagtaaatag ttcagatgaa
      301 tttgtattta taggcattac tggtgctcgt cctaaaagac ctccaaattc taccatatca
      361 cctacatcac atcctggggc tggtataatt ctaactgctg tagtcttatt attaatcata
      421 cctattgctg cctcatcagc tatcatggct ccaagcgtac ctgctgtagt atttcctggc
      481 acagctatca tgtcaagacc tacagaacaa atagcagtca ttgcttcaag cttatctata
      541 cttaaagcgc ctttgataac agcatcaatc atacctgcat cttcagatac tggtataaaa
      601 gctccactaa gtcctccaac atgtgaacat gccattactc cacctttttt aacagcatca
      661 tttaaaagtg ctaatgctgc tgtagtccca tgagtaccaa caacttcaag acccatttcc
      721 tctaatatgt ttgctactga atcaccaata gcaggtgtag gagctaatga taagtctatt
      781 attccaaatg gaacattcaa tctactagaa gcttcctttg ccacaagttg tccaactctt
      841 gtaattttaa atgctgtctt tttaactgtt tctgaaacaa catcaaatgg ctctcctttt
      901 actttctcta gagctctctt gacaacacca ggaccactta caccaacgtt tataattcta
      961 tcagcttcac caacgccatg gaaagctcct gccataaatg gattatcttc tactgcatta
     1021 caaaatacta ctagttttgc acaaccaaaa ccttttgcat cttttgttaa ttctgcagtt
     1081 tcttttatga tttctcccat ttctctcact gcatccatat ttataccaca tcttgtagag
     1141 cctacattta ctgaagcaca tactttatta gtacttgcta aagcttttgg aattgactta
     1201 attagtatct tatcaccttt tgtatatccc ttttgaacta atgctgagaa tccaccaata
     1261 aaatctatac ctaaatcctc tgcagcctta tccaatgtct gtgcaaactt tacatagtca
     1321 ctttcatctg ttgcacctgc aataatggaa attggagtta ctgataccct tttatttaca
     1381 attgggatac caaattcacg ctctatatcc tttccaacct tcaccaaatc tttagcagag
     1441 ctcataatct tattgtatat tttttctcta gctttatctc catcagggtc tatacaatct
     1501 aataaagaaa tccccatagt tatagttctt atatccaact gttcttcatc tatcatcttt
     1561 attgtttcaa gtatatttcc tacattcact gttatcacct ccccttactg tctactacag
     1621 attatgcatt gaattaaata tttcttctcg ctgaattttt acatctactg ataatttctt
     1681 acctctttca accaagtcct caaccgtttt ctcaaaagaa tttaatgatt tttctaagtc
     1741 aactatcata atcatagtaa aataatcctg taatatcttt tgtgatatat ctactatatt
     1801 gatattttgc ttgtataatt catcactaat tcctgccact atacctggtt tatcctttcc
     1861 aattactgtt aaaattgctt tcataataat atcctccctt atttttttat gaatcttata
     1921 tttttacaaa ttgaactatg tattaacata catttcattt atttgttaat aaatcacttt
     1981 ttatgtatat atttaatata ttttaatacc tatacattca tttgtcaatt ttttttcaat
     2041 attctattat ttttatacta tttagaaaat tcacgtatta tatatgtttt ctcaacatta
     2101 aatacagttt tcataataat gaaaggtatt acttagtaat ctcatgagtt tataaataat
     2161 acctttctaa aaattatttt ttttctttta tgaacttaat tatacttttt ctagtatcag
     2221 aatttacaat taatatagaa ccaatcataa ttggtgttag tatgcaagct aaaaaaataa
     2281 tgtctttttt tagattatat tctttagatt ctaatttata attattattt attattccat
     2341 tgtaatcact tatatttgac ttatttttat ttgtatcttg atttttatta gaaatacttt
     2401 ctctataagg aaaagaatgt tcattgatgc caaatattga ttttaaattt aaatcataat
     2461 caccatcatt ttctataata gagcttttat tgaaaactcc tttttcttta tcagaattat
     2521 tttctttatt accatcttta ggtatattac cttctttggg tatatcacct tgaggatttt
     2581 tattttcatt gctaccagta cttgattcta catcatcttt tggagcattt ggttgttttt
     2641 catcagaatt atcattttca ttattattat tttgattgtt attactatca ttattatcat
     2701 ttccattatt tccattttga ctgttatcat ccttatctct atcagggtcg tctctacctc
     2761 ctcctccacg gtcaccatca atatcatcat tttcctgttc atcatctggc gtctcttggt
     2821 tgtcgccacc tcccattata aaacctggtt taaaaactat atttgaatat ccttctttta
     2881 atcctcctac cactcttatt ctaaagcaac gcaacttaaa atcttcaaag aaaagtatag
     2941 cttggtctct aatatcctcc tgttcttcag atatccaatt gattccgtcg cttgaatatt
     3001 caacaaaaac cttacttgca ctatacggca tgtcataaaa taaagcagct acatatcctt
     3061 ttttcgtatt ttgaaattcc aattctaaat tgtctattgg ttttttatcc ataacatata
     3121 tattcaaaat aggaactaca tcatcattta ttataatttt ttctttattt aatgtttctg
     3181 ttttgaactt tcctgttatg ttaaattttt ctcctttttc taatttttca taataatcag
     3241 taaaatccca ttctacatct atatcaagtt gattaccact agatgtttct aataccatat
     3301 attgtggaaa ttttatattt tctattttgt catctttact tacttttatt tcttcactaa
     3361 atacaccaat ccccctataa caacatttca caatattgta gccagagtct tcagctatag
     3421 agggtataag ttcattataa ctatcaatta tataaacttt cttgctttct gatataattg
     3481 attgtaccaa cttattatta tctcttaata actcattttc attgttgtta ctatatgcct
     3541 ttattgaaga ttcttctatc tcaacatcac ctttagaata aacacctata gcacgctctc
     3601 cagtcacttc aatatcctta tttattattc taatatcatt ttcagtataa attcctattg
     3661 catcttttcc atttgccttt attttttcaa cagattcata ggatgattta atatataagt
     3721 ctcctccttc ctcaacgtat aatgctgtcc cgtcagtagc aattatgtta tttataccaa
     3781 tacttatact tccaccattt tctacatgta ttactccttc ttttcctcct tcacctatta
     3841 tatttagttc attatcatca ataataaatc tcccatgatt ttttatcaat attttatatt
     3901 tgttagtatc tattgtaaaa ttgctgtcat aaggaatata aaatctaaac tcttcgtcta
     3961 ttaccatatc gcaagaaaga gtataaacta ttttttcttt attattttta ttttcttgaa
     4021 cccacctttc aaaatcttca agacaatttt cctcttgtcc aaaaacttta tatggaaaac
     4081 ttataaaaca taaaactact atacttataa aaataaatat ctcatatttt ttcattttac
     4141 actcctagaa attttttagc tttaaacaat cttgtcgaaa catatacttc acttactata
     4201 atttttccat aattatcaat tattgtcaat ttatataagt acttttatta attgctataa
     4261 ataaaaagct acttactaaa gtaaacagac gtttaacttt aataagtagc tatattttta
     4321 taacaaaata gttataatat tagaacttcc ttcatttaca attttttgta aagtcttttg
     4381 catcttgact cttatttctt ctggcatagt atataattta ctttgtaatt gttcttttac
     4441 taagtcatgt aatgatttgc caaacatatt tgattcccat aagtctgctg gttgctcttc
     4501 aaatacttcc ataagatatt ttatcatctc ttcaccttga ttttgatttc caactattgg
     4561 agaaacctct gtcgatatat ctgcttttat aatatgaagt gatggtgcat tggcttttaa
     4621 cttgatacca tattgttttc cttgtttcat tatttctggc tcttccagac taagttcttc
     4681 taaagaagga gctactactc catatccttt tatcttggca tctattaaag cactttctat
     4741 tttatcatac tcatttttaa ctttagatag tctagttatc agactaagaa gttgataatc
     4801 tccttcaatt ttaaatccac ttttttcttc aaggacatta tagaataaat cttgctttgt
     4861 ctgcaaatca atatttataa caccttctcc taactctaca ttatcaacac ctgtatcttc
     4921 caaaaactct aattcagaaa atccttgcat aataccttct atatctctta ttttacctat
     4981 atctataata ctttgtttta atgttgttat tatgctactt tttatccaat gatttctttc
     5041 aagaccttca acccattttg gtaggttgat tcttatttct gtaagtggaa aatcatataa
     5101 tacagcttcc ataacctctt ctatatcttc ttcttccatt tcaaccacat tcattggaag
     5161 aactggaact tcatactttt cttccaattc acttctaagc atgctcgttt cctcactttt
     5221 tggacttaac gtatttaaaa ccactgcaaa tggtttttta aggtttttca attcctgtat
     5281 aactctctct tctggctcta catagctctt tctgtcaatt ccagtaactg aaccatctgt
     5341 caagacaact atccctattg tggaatggtc ttttataacc tttttagttc ctatttctgc
     5401 tgccttttca aatgtcatag cttcttgtga ccaaggagta gatactaatc tttgttttcc
     5461 accttcctca tgccctaaag ctccttcaac tatgtatcca acacaatcta ccattcttac
     5521 ttttaaagat actgtgtctt ttatttttat ttctactcca tctgctggta caaattttgg
     5581 ttctactgtc attattgttt tacctgaacc actttgaggt atctcatccc ttgttctatc
     5641 ctttttaaat tcattgtcta tattaggtat aaccaacttt tccataaatt ttcttataaa
     5701 agttgatttt cctgttctta caggtccaac tactccaata tatatatccc cctgagtcct
     5761 tttggatata tcttcgtata tgttattatt catattattc ctcctataca tctaatctta
     5821 ttaaattata tttggatttc gaggttaata ttcttaaaat ggaaaaataa agaccagcaa
     5881 tattaatttc gtttacagat agaaattctt tcattttctg ctctatagtt ttcataccaa
     5941 acctgtctac tatgtattac tatatctcct ctttgaagta ttctagtatt ggagtctaaa
     6001 tgaagttgtg ctgttctatc aatccctgaa caaatacagt taaaaaattt tatatacata
     6061 taaggatact tttaagaaac atattttagt aaagcattgt ctcctacatc ttttacatat
     6121 ctcatttcaa atattttact tccaacatct ttgataacaa atgatttatc agtcatatac
     6181 tctgaaaaac ttgcagatac tatatgtttt tctattccaa cagttgttcc cttaggattt
     6241 atataacaaa acaatattat gatatgtttc cagaagattt aggtgttcat gatagtgaca
     6301 tatctttaat gattacagga gaaagtataa aggaaagaaa taagatacta ttaaacaatc
     6361 ttgtagatac tggatatata ccagctaaag atttagacat aattaatgat ataattattt
     6421 ctttttatca aaatctatta ttcttaaaaa agaacacagg aaaaaattct gatgatgatg
     6481 aattaactat gaaaacgatt tcttctattg aatacataat aaaacatgct aaataaaatt
     6541 aaaatatggc tgtctcaaaa tagagataag ccttttttat tgttgaaata ataaaaatac
     6601 atttaatttc atacaaaaag agtgtctcat aaactaaaaa gttcattttg agacacccct
     6661 aaaacaaatt tattatttat tatacaatag tttatatatc atcacctaaa actacttctt
     6721 ccatttcatg ctttttactt ctcatcatta gttctattcc aacttcttta ggatttgaac
     6781 ctttctttat tacagaatat atagcattag ttataggcat atctatatct aatttttcag
     6841 cgacatcatg tgctacttca gtagcagtta taccttctac aaccatcttt acttctttta
     6901 aggtatcttc taaactcata cctttaccta tgagtatacc agctcttcta tttctactat
     6961 gcatacttgt gcaagtaact attaaatctc caatacctga aagacctgca aaggtagaca
     7021 tattagctcc catagcaatt ccaagtctgc tcatttcact aatacctctt gtcataagag
     7081 ctgcttttgc attatcacca tatcctaatc catcacatat cccagctcca aatgctataa
     7141 tattttttaa tgctcctcca agttctactc caactatgtc tggattcgta taaactctaa
     7201 gtttagggct cataaataag tcttgtatca tctgtgctat ttttaaatct ttagaagcaa
     7261 ctactacggt agtaggaata tcccttgcta cttcttcagc atgagacggc ccagatagta
     7321 ttacatatgg attttgtggt aattcttctt cacagacttg agataaccta agccaagtcc
     7381 ctttttcaag acctttggct acattaacaa taacttgtcc ttcttttata aatggcttta
     7441 ttttttggca agtacttctt acagcctgtg atggaactgc taaaattatt accttacaat
     7501 cttttatagt tttttctata tcagtactta tagttatatt atttggaaat aatactcctg
     7561 gtaaatagtc tgtattttct ttagttctgt ttattttttt agcttgttcc tcttttcgag
     7621 tccacatgct cacatcatta cctttttttg caagccctaa agctaatgca cttccccagc
     7681 ttccagtacc taatacacat actttctcca tgtttaacac cacctgagta taatatattt
     7741 actattcttt cttttgacca atttttctct ctgtcccatt taataatcgc tttatatttg
     7801 ctctgtgatt atatattact gatgcagtta agaacaaagc tactattaaa ccagctttat
     7861 tttttaccat aatcataaat attggtgagc atcctatccc tacaacagaa cctaatgaaa
     7921 catactttgt tataactacc accaatataa atactgctag acacattagt gttattactg
     7981 gatttacagc tagcattgcc cccagcgaag ttgcaacacc tttacctcct ctaaatccta
     8041 aaactgctgg ccaattatga cctgccacaa cacatataac tgccagatat ccagctaaaa
     8101 gagtatctat tccaaccaat cttgctgcaa actctgatat taaaacagcc atcacacctt
     8161 taagaacatc acctaaaaat gtcattgctc ctgctctttt tccaagtgtc ctaagtacat
     8221 ttgttgaacc tgcgttacca gaaccttgag tccttatatc aaccccagca attcgctttg
     8281 caactatgta tgaggtagat atatttccta aaagatatgc aactacagca ataattatat
     8341 aactaaatat ctccactgaa tttcccccct ttagttatat tttagatatt tattttttat
     8401 ttttttgtct atactcaaac tttatggaag ttccttcaaa tccaaaattt tctcttatct
     8461 tattctcaag gtatctttga tatgagaagt gagttaattc cttatcattt ataaacaatg
     8521 taattttagg aggtctaatg tcagtttgag ttccatagta aatttttaat cttctacctt
     8581 tgtcagatgg tggttgattt aacattacag cttctccaat tacttcattc aatgctgaag
     8641 ttgatattct cttagaatgt tcatttgaca catattcaac tgtatctagt atcttgttca
     8701 ttctttgatt agtctttgca gaaacaaata caattggagc atacatcata aatggaaatt
     8761 tttcttttat atcttttgtg taattgctca tagtcttatt atctttttct attaaatccc
     8821 atttattgat tacaaatatg catccttttc cttcatcatg tgctatacct gcaacctttg
     8881 tatcttgttc tgttacacct tctaaggcat ctattactat caatactaca tctgctctat
     8941 ctacagcact catagctctt attacactgt atttttctat tgtctcatat attttacttt
     9001 ttcttctaag acctgctgta tctattagaa gaaatttctg tccatttttt tcaaaataag
     9061 tatcaattgc gtctctagta gttcctgcaa ttggacttac aattactctt tcttctccaa
     9121 gtatcttatt aagtatagaa ctttttcctg catttggttt acctgttata gctactctta
     9181 ttatatcttc ttcatactct gtatctaatc ctgctggaaa attttcaact atctcatcta
     9241 ataaatctcc aaatcccata ctgttggctc ctgaaactgc aaaaggcgtt ccaaacccca
     9301 actcataaaa atcgtatata ttatcaaatt gactttgact atcaatctta ttaactacta
     9361 agattactgg tttttttgtc tttctaagca attgtgcaat ttctttatca gcagctgtta
     9421 ttccagcctt accatcaact ataaaaagta ttacatgtga catgtccatt gcaagcatgg
     9481 cttgatttct catttgagat aatattatat cttcgctatc aggttcaatt cctcctgtat
     9541 ccactaatgt aaaatattta tctaaccatt ccacttcagc aaaaattcta tctcttgtaa
     9601 ctccaggtgt attttcaact atcgatatcc tttttcctgc aaatttatta aatattgtag
     9661 acttaccaac gtttggtctc ccaactacag ctacaactgg cctacttata ctcattttgt
     9721 ttctccttcc atcttttgtt ttctatttta aaattttatc cacaaagtct ttaccttcat
     9781 tcaagcaagg gataattttt attttcataa gttcttctaa ttgatttaat tcaatattat
     9841 ctaagaaaat ttcttcatct gcttttagca tacttcttgt tatataaaga gcttctccta
     9901 attctttatt ttcaagttgt tcttttaaat ctttagctgt taaaagacca gatacagtta
     9961 ttgtctctcc aaaaaattta tttttaattt catagacatt tatttgaaga tttttaaact
    10021 tgtccatcat agcatttgcc atactctgta taaattcata tgcagaatga ccagtagcta
    10081 ttgatacttt cttatctctt tttaaaatat cctcaggcaa tatatttaga tactcttcaa
    10141 tctctgtttt taacttacgt atcataccta caccatcttc aaactgtaaa aaaccttcat
    10201 attcttcata tccaggcaat tcactattag atagtatata aaattcatct gataaaaatg
    10261 caaatcgagt acctaattgt ttaagatatt tctgttgcat ttgatgtatt tgctctatag
    10321 ttttacttgc gctcttacca ttaaatatat ttaattctac tagattttct ctatgctttg
    10381 taatcccaac tggaactata gcaacactat ttacatacgg ataaagttga gctagttctt
    10441 ttattgttct atccaattcc tttccatcat ttatgcctgg acataaaaca atttgacagt
    10501 tcatttctat atgagcatct gccaatctct tcattatacc atataatttt cctgcaaatt
    10561 tattacttat cattttttgt cttaactctg gattagttgt atgcacagat atatttatag
    10621 gacttattct atattttata atattattta tatcttcttc actcatattt gtaagtgtga
    10681 caaaatttcc ttgtaaaaaa gacaatcttg aatcatcatc tttaaaataa agagtttctc
    10741 tcataccttc tggcaattgg tctataaaac aaaacataca cttatttcta cagcttttag
    10801 cttggtcaat tattggattt gtaaattcaa cacccaaatc ttcatcatag tctttttcta
    10861 tctcgtatat gtagacttca ccatcttttt tttgtatttc tacctctaaa tattcatcac
    10921 ttagtaagaa tctatactct attatatcat gtattggttg ttcattcaca gatataagta
    10981 aatctcctac ctctatacct atttcttctg caatactatc tttgtaaacc ttacttataa
    11041 tattatttac tttttttaca tttacttcca atattttcac cactcttttg tattcatcaa
    11101 tgcttattat aatatatttt tatatatagt ttacttattt attatatttt atggtagcat
    11161 ttttaagcat ttcatgtcat aataacacat tttaattcta atagtcaata taagtagata
    11221 gctataaact agattaaaac aaataatatt caaatttaag ttttttaaaa atttgaatat
    11281 tatttgttct taaaaaattt ataaatttaa tgttttacta tgattagcgt cccattcgtc
    11341 atatcatgaa ccattccacc tacacacttt gacatcaaca atgccttatt ttgtaagtct
    11401 ttctcatcgt ttgcataaaa tattggacaa cctccaccat ttttcatatt cttatcaagt
    11461 gtaactatag ctagggtata ttcatttaaa cctatatcca tccccatctt atgcacttcc
    11521 tttctttcct attttataat tttttagtga taagtttttt cctttagagc ttgaaagtat
    11581 tgggcatgac tttacagctt ccattacttt ttctacattc ttatctactg gtaaataagc
    11641 aatcattata ctttcatcct ctttatttct tctaggtaat ggtacaaatg aaggttctcc
    11701 ataatctcca tataaaccta acctcgaata tatattgtat actattgctt gtctttgacc
    11761 agaatcaaat aatattcctg cattagcata atctttattc ttaggaatta tttgtattcc
    11821 tacacctcta ttaatatata cttctctatc actttttaga cctatatttg ttataccttt
    11881 taaatctcca acctttagta tagactcgtc cacaaaacta atctcagcag gcattacatc
    11941 agctatgtct ccaatacttt ttctagtaag gagttttttt aatataaaag ctagcaataa
    12001 tccacaaatt gaacttatta ccaaactcat cccttcactt attttcgttt ctgcaagtat
    12061 tacatagtac aaccctattg ttaaaaaaga ggtaactatg cacatataat ttctcacttc
    12121 ataggttcta gctatctctt ctatgaatgc agcacctctc tttacaagct gaacttcttc
    12181 taaattttgc aacgtatttc tcctatttgc tctcacttgt ctaaattgtt ctgctgctag
    12241 agataaaaat gttatagatg tataagaacg atttataaga gctggaacca ataatgctcc
    12301 taatgaagaa gcgacgaatg ctaatataag ttgagatatt aaaacatttg gttgcgttgg
    12361 atactgtttt tggtctaaat tcaacattat aattctagat gttataccaa taataacagc
    12421 aacaaaaaat actctatcca ttaaatcact cctaaaatat tttagtttaa aattttacat
    12481 gtaaatctac aaagtctttc tagaaaaatc actctgcttt ccttttgatg tctctatgat
    12541 aggtgtactt ttcactacat ctatcatgac gtctatatct ttgagtattg gcatatacgg
    12601 tataaccaca gtacgttttt ccaaattggt cttagatata gcaacaatat caacctcatc
    12661 tacatctcta tctatcccca tatgaataaa tagattatgt attatagctt gtctttgccc
    12721 tatatcgttt actattccaa atgcacctaa atctcttggt ataacttcta tcgccagaca
    12781 ttcatttcta tacttttctc tcgtactttc tagacctata ttggttatta ttacatcatt
    12841 taccattaaa ataggacctt caaagcttat ttttgcagga actatatctg ctatatctgc
    12901 aatactgttt cttcttaaaa atcttctaaa aatcaaacca acaatagctc ctgacacaat
    12961 agctaagatt gtacaataaa aaaagctaag gtcgtacttt cttgcaaaaa cgatataaac
    13021 tatcgaagat actagtgcag aaaaaagact tatataactt ctagattcat acgtagatgc
    13081 tatttcttca acgtaagcat ctccttttgg tactaattct tcattatcga tattctttag
    13141 tgttattctt tcttgttccg caagaccttg aaactgctgt attgctactg caaaaaatgt
    13201 caaggctgca aactccttat ctataagtgc aggcaatgct atagcaccta atgaagctga
    13261 aagcccagat ataattatct gttctaaata atcttgtggt cttgatgggt attgtttgtc
    13321 tgtaactctt aaaactagtc ctctacataa tattcctacg actaatgcaa ctataaaaga
    13381 gtgtctaaat aaatttgcat ttaataaact ttcttccatt ttctcagctc cttctcttat
    13441 atagtttgtg caaaaaacaa aaaaaatttc cttctattta aggaaatttt tttgtaatat
    13501 tgtaactata aattcgctac caatgccaag ttcacttttt acttttatac atccatttag
    13561 tgatttaact atatgctttg ttatagccaa tcctaaaccc gttcctccta catccctact
    13621 tcttgcttta tctaccctat aaaatcgttc aaatatgcgt tctacatctt cttttggtat
    13681 acctacacca ttgtctataa ctttaattac tattttttct tcatctttta acactacaac
    13741 ttgtacaatc ccattatctt gggtatactt tatagcatta tcaactagat ttaagaacac
    13801 ttgctttata tagtctttat tagatagtat acttattgat ttatcatcac atttatataa
    13861 aacttctata tttttttcac tagctgaatg ttgagttatt tcatatactt ctttaaatac
    13921 atcatacact acaacatttt ccataagaag agtatcttta ttttctacaa acgataacag
    13981 caatatatca tcaatcaatc tttttaatct atctgattcg ctttctatta tttctaagaa
    14041 tctatttcta gtattcacat ctatattttc atttgacttt agtgtttcga caaatccact
    14101 tatagatgta agtggtgttt taagttcatg acttacattt gcaacaaaat cacttctcat
    14161 attttcaagt ttaacttttt tagtaatatc ttctatattt actatcgaac ctattattac
    14221 gttgttagta ttttgaagat aaactgggtc tacttttatt ttatatacta tatcatcttt
    14281 agtagtaata ttggcagttt cattatcttt agagccaata aatcgaagta tttgttttaa
    14341 tatttcttct tcttttatta caacattaat attatgacct tcaactaatt gatttggttc
    14401 actctttatc atttcttttg cttcatcatt tatcagaagt atattgccat ttacatctat
    14461 agccaaaatt ccatgagata tactctttaa tatagacttt aactgtaaat gcttatactg
    14521 cacttcttct aatgtcttat ccatattctt tatcatatga ttaaaatttc tagctaattc
    14581 accgagttct cctttagctg atatattaag cctagcatga tattcattat tacttacttt
    14641 ttttgagacg tatataaatt cttttaaata tttccttaat gttattgtgt atcttacagc
    14701 taatatcaca atagctatgg acaacataac aataaaaaaa tatacactat ttaaatcttc
    14761 cacaagataa cactcctaaa atataacctt tttaatctat tttgtatcct acacctctta
    14821 tagtctctat atatttttct gatttatctt catcttctat tttttttctt aaatatctta
    14881 tatgaacatc tactgttcta gtttctccat agtattcata tccccatatt ttatccaaaa
    14941 gataatttct tgaaagtact tttcctttat tttgaatcaa tagttttaat aattcaaatt
    15001 ccttaagagt taactctatc ttttcagaac ctttggtaac ttcatgtttt gataaatcta
    15061 gttttaaatt acctgtagta agtatattgt tttcttcacc taagctagtc aaattatatc
    15121 tccttaaaac agaactaatc ctagctaaca actcttttat actaaatggc ttagttatat
    15181 agtcgtcagc acctatttca agcccttcaa ccttgtcatt ttccatattt ttagcagtaa
    15241 gcattataac aggaatattt tttaagtcct tatcacttct tatttttctc aaaacttcta
    15301 ttccacttat atttggtaac atccaatcta aaagtattaa atctggttta atttccttgg
    15361 cctttataaa tccatcaaat ccatcatatg aataactaac ttcgtaattt gacacctcca
    15421 aattgaattt taatagctca actatatgca tttcatcatc tataacaagc actttagtat
    15481 tcattaaaaa gctcctctct attttatctg tagtaccgtt cctattcgtt ctgatgcctt
    15541 attatgtttt ctataagagt ggaatttatc tgctctacaa ctagtgcata aatttaaatt
    15601 tattatattt tcttttttaa ctccactaca tataagcata tactcattaa ttttccataa
    15661 atctaaatag taactcccct cttttattat atagaacttt tcatcattat ttgtaagaat
    15721 tgtgttaaat ttttctacta aatcttcaga cacttcataa caacaaggtc caattgatgg
    15781 cccaattata catactaaat cttcgggaac tgttttatag cattcagaca ttaattttat
    15841 tgttttatga cctatattac taaaagtacc tctccaacct gcatgagcca gtcctatggc
    15901 tttattttta gcatctataa ttgatatagg tacacagtct gctgtaaaca gtaaaagtgg
    15961 tactttttcc aagtttgtta taagtgcatc cccatctctt cttttaccta tggtgtcttt
    16021 attaactatg ttaactatat ctgaatgtat ttgagaatta ctagttaaat tttcaaatgt
    16081 caagtcacat tctttacata cttttaacat atcttctcta ttttttgcat ctatattctt
    16141 acatgttata actagattta cagattttaa cttttcatca atttctttac aattaccact
    16201 taaattaaca tactctttaa tatcttgtac attttttata tttatataat ctttcatcat
    16261 ttgtctcctt tttctatcaa tatactttta agttcattta caaaagtatt tatatcttta
    16321 aattgtctat acacagaagc aaatcttaca taggcaactt catctaaatc tttcaattta
    16381 tccattacca tctcaccgat ttttttggtt tcaatttctt ctataaaaca tttatttatt
    16441 tcattttcaa tatgtacaac aatgctttca atctcttcaa tagaaacagg tcttttttca
    16501 catgccttta acaatccata ttttatcttt tctctatcaa aatattctct actattatcc
    16561 tttttgatta ccattaaagg agttgtttct attttctcat acgtagtaaa cctcttttta
    16621 catgattcac actcacgtct tcttctgata gattttaaat ctgtatgtct agaatctatt
    16681 acctttgatt ctttataatt acaataagga cattgcatct atattcctcc tttgtttaac
    16741 tatatctcaa actcatctaa gcttaattct gaacctatat ttactattat tgtatcacaa
    16801 cctattttaa gtatatcatt ccaaaatata acttcttcat cccctcctct tgaaaaaaaa
    16861 cttctacttc catctccaaa aatggtaaat gagactactt taccttcgtg aatatccatg
    16921 tctatgtcta ttataaaccc catccttttt ccatttttta catttataat ttctttttcc
    16981 attatatcag aaactctaat catattgtac tcaccctttc tttaaaaata aatatgtatc
    17041 gaagtaaaaa aaaatgacct atataggtca tttaaattaa aataatgttt acagtccatg
    17101 gattacttat acatattttc tcatattttt taaagcattc ttttcaattc ttgatacttg
    17161 tgcttgagat ataccgattt catcagcaac ttctatctga gttcttcctt tgtaaaacct
    17221 taaatctaat accaactttt ctctactatt taatttctta atagcttctt taagtgatat
    17281 ttcttgcaac caattttcat cagtatcttt tttatcttga acctgatcca taacgaatat
    17341 tgcatctcca ttgtcttgat acactgggtc aaacagagat atcgggtctt gtatggcatc
    17401 aagtgccatt acaaccgact ctacttctaa ctctaattcc tttgctattt ctgatactgt
    17461 aggttcttta gaatttgttc taattaatct ttctctaact tgtagtgcct tatatgctat
    17521 atcttttaat gacctactta ctctaattgg gttattgtct cttagatatc ttctaatttc
    17581 tcctataatc ataggaacag cataagttga aaatcttacg ttttgactta aatcaaagtt
    17641 atctatagct tttataagac caatacaacc tatttgaaat aaatcgtcta tattttctcc
    17701 tctgttgtta aactttttta ttacactcaa gactaatctt aaattccctc ttacaaactg
    17761 ttgtctggct tcttcatcac cattttttat ttgtaataaa agctccttca tttgcttatt
    17821 tttaagaact ggaagttctg atgtgttaac gccacatatt tcaaccttat taacttgcat
    17881 atattttcag tcctctctca aaagtattca tctattgaaa ttatttacat ttccaatttt
    17941 tttatacttt ttaagaagaa ttaatttaac attatacaaa ttttttcatt tctttttgca
    18001 accttgaaat tattttcttt tcaagtctag atatgtagga ctgtgatatt ccaagcattc
    18061 ctgcaacttc tttttgtgtt ttttcgatac ctttatcaat aagaccaaat cttaattcca
    18121 taatttgctt ttctctgtca cttaatctgt caagtgccat aaccaacaaa tctctatcta
    18181 tttcttcttc aattattttg tatatttcat cattttctgt acccaataca tcagataata
    18241 aaagttcatt tccgtctaaa tctatattta aaggttcatc aaatgatact tctgttttct
    18301 ttttattatt ttttcttaag tacattaata tttcattttc tatacatctc gaagcataag
    18361 ttgccaattt tatattttta ttcagtttaa aagtattaac tgcttttatc aaacctatag
    18421 ttcctattga aatcaaatct tctacatcta ttcctgtatt ttcaaatttt ctcgatatgt
    18481 agactactaa tctaagattt ctttctatta aaattgactt tacactttca tctgtttcta
    18541 gcttttgtaa aagttccatt tcttcttctg gtttcaatgg aggaggaagt atatttgccc
    18601 ctcccatata gtatattccc tttggcttta ttatttttaa tttaatacct aacatagtaa
    18661 taaagcttat aattctttct ttcaatcgta acattccttg tcccccttaa attttcacct
    18721 aaaatccagg taatatattt ggattcatta ttgctccata ttctctatca ttaaaatttg
    18781 aaataccaag tattatattt cctatctttt gttcatctac ttctatataa tcagctttta
    18841 aacctattat tatgcttgtc ttattgcttc cagcatgttt ataaggtatt attctaactc
    18901 tacctgaagt tttatcatct aaacttccta taatttcttg tgcttttttc atatctatat
    18961 cttcataatt aatatttaat agtttttctg gaataaatcc ttctagcaca cttgacttaa
    19021 ctattattac atcactttga cttattgggt cttttaacaa attaccacta tctactaaag
    19081 ccttacagca aaaacttttt tctagtaatt ttatatttat agtctttgta agctccttaa
    19141 tgtacttaag cattttcaaa tctttataaa tgtattttaa taattcacaa ctcacataag
    19201 tacatgcaat taaaaaagat atttttaagt gacttattcc tgtaaaataa attataaaat
    19261 aagtgcttcc agaaataaaa atgtttacta gataaaaaac taaagctaca tgaacataat
    19321 cttttttgct cttgcttcta aatgaaatta aagttattat actcatgatt attattttaa
    19381 atggtatagt aaatagtata ttcattgatg gatacaaata agcaacagaa taggcacctc
    19441 ctaaactagc ccccaacaac ttattttttt taggattata tcttttagta agaatagaag
    19501 tacaacttat aataatataa tttattacta ggttttctat aacatagtac tcaatataca
    19561 ccatgtatcc ccccttaaaa cctttaaaca tattataaga tagtatgtac aaaattttat
    19621 gtcacttttg atggtcgaat ccaaaacaaa ttctatttaa aaagaacaat gatatatgtc
    19681 ttatacttta tgtcgccaaa agatattttt ttaatgccaa acaaataggc ttttttgtag
    19741 aatccaatat atctaggtag aagttattcg ttctactttt ggtatatttc cataaaaaaa
    19801 agactacata atttaataat atatcaagta aatcttttac ttatctaaaa ttaaattatg
    19861 tagtctgtaa attcaaatat aaatttaaat tattgtgcta ataatccatt tgcatatttt
    19921 gcagattcat ttagtgcttg ttctggagtt atgtctccag ctatcatcat ttgaagttgt
    19981 ttccataagt attcatatat ctctactcca tgaggtccaa ctgttaatgg tctatacata
    20041 tgtttatctc ttgtaaccat ttcttcaaac cttgggtctc cttggtattt ttcaccttta
    20101 gcaactggag ctctaggtac ttctttgtgg aaagcttcca ttgattcttt gctcaacatg
    20161 tattcaagaa gtttaaatcc aagttcttta tttttacatg ctgacattaa tgttaaatca
    20221 tctactgaag caaaacttcc aactgtctta tcttctaatg atgttacata tccccagttt
    20281 aaatctggga atgatttatc aaatgtactt gtagcagcag aagacaacat tatagtaaat
    20341 gcagcttttc ctggtccaaa tacactttca atcatttcat tactatcttt tgacaaagca
    20401 tcttctggaa tatacttttt catatcattt ataaagtttg cagctttcaa accttctgca
    20461 ttattaaatt taactgattt taaatcatca ttatatatac ctccaccagc ttgccataaa
    20521 tacggatacc agttccagtt taaactccca aatactttag aaccccaacc ttgtgcaaga
    20581 ccccactggt ctatttttcc atctccatca gtatcttttg ttgctttttc acatattctt
    20641 ttaaaatcat cccatgtttt tggaggtttc tctcctaatt tttctagtat atctttattg
    20701 taatacaata cacctggatt agcagcttct ataggtaaac cataaatacc acccatcatt
    20761 tttgcatcat ctaagtatag ataattatct gaagttcctg atttttctag ataaggtgtc
    20821 aaatcttcta ccgcacccat ttctataaat tgagggaaca tctctgcata catgtatcct
    20881 atgtctggac cttcacctgc acttattgct gttgcatatt tttctgcgta gttatcccat
    20941 ggtactatct gaaattcaat ttcacagtta ttttcttttt caaatttttc aaatatcggc
    21001 ttccatgcat ctgcatcatg gtctgttaat ggtgcagacc acacaactaa tttttcctta
    21061 ccctctttgc ctttagaatc ttcttttttt gaacaccctg caagaattga tactgacaga
    21121 atccctacca taactaaaga aagaaccttt tttagtttaa tcatgcttaa tcccctttca
    21181 gactttaatt tgtctttttc ctcaaatgtt tacatttaat tcagatttaa tattaacaaa
    21241 taagactact cttgtctaat cacaaaaaac tatactaact accataacta tatttttttt
    21301 ctatacatat tagatttaat ttacactaaa aaacttactt tttaaatttt gtcaatattt
    21361 aaaagtgttt ttaatcctag ccacaaacta actatttaaa ttgagcaaaa taaaaagaag
    21421 tctattgact tctttataag cttacatatt atcttcttct tcttaagaat gtaggtatct
    21481 ccatactctc atcatcatct tcttcaatta tacttgcctt tttaggaggc tctatttttt
    21541 cctcagcaat tacttcttct tcaatttctt ttacagcttg ttttactgtt gtatttaatg
    21601 aggaacgtat gcttgattta ggcttagttt ctaaatccaa ggcaccattt tgtaatcctt
    21661 caaatcctgt tgcgattact gtaatcatta tttcatcacc caaatcttct tttatagaag
    21721 ctccaaatat aacatttgct tctgggtcac aggattccat aactaaagtt gatgcctcat
    21781 taacttcaaa taatcctagg ttaggaccac ccgtaacatt aagaagaaca cctttagctc
    21841 cttgtattga tgtttctaga agtggagatt gaatagcttg tctagcagca tcaattgctc
    21901 tagtttcacc actagcacta cctataccca tatgagcaag tcccttgtct ttcattatag
    21961 tagtaacgtc tgcgaagtcc aagtttatta acccttcaac tgctataagg tctgaaattg
    22021 attgtatacc ttgttttaat acatcatctg caactgcaaa agcatctaac attgatgtat
    22081 ttttttgtac tatctgtaaa agcctatcat ttggtattgt tataagagta tctacttttg
    22141 atttaagctc tgctattcca ccttcagcat tcttcatcct tatcttacct tcaaatgcaa
    22201 aaggtttagt aactacacct actgtaagta tccccatttc ttttgccaag cctgcaacta
    22261 ctggagcagc tcctgtacca gtaccaccgc ccattccagc tgttacaaaa accatatcag
    22321 ctccttgaag caactttact atttcatctt tactctcctc agcagctctt tttcctactt
    22381 caggatttgc tcctgctcca agtcctctag ttaatttttc tcctatttga actttatatt
    22441 cagcttttga tgtatataaa gcttgtttat ctgtatttac tgatataaat tcaacgcctt
    22501 ttagttgagc ttctaccatt ctattgacag cattgtttcc accgccacct acacctatta
    22561 ctttaatttg tgcacattct tctaattcta cgtcaaagtt tagcatcata aacccccttc
    22621 tctcatgatt caaagttatc gtatgtatta attatatcat tttcttcttt tattttgtta
    22681 acctttaagt aacatattcc acaaaaacgt aaattttcct ttttaaattt cttacttata
    22741 ttgtcaaata ttactcttta agtataaaat aatttacgta tatattaaat gctctttaat
    22801 ttttacgttt tttattcata atattactaa taatatatcg tctaattata gataagttat
    22861 taaacaatct cataccaaaa actaatacag caacataata aagagaaatt cctaactttt
    22921 ctcctatata agtcaataat atagccaata tagcattacc tatgaaacct gtaataaaaa
    22981 ttacattatc atagttatct tcaaaattgg attttatcgc tccaaaaata gaatccatta
    23041 ttgcaagaat agctactgac atatatatgg aataatccat agaatatgta aatggaatat
    23101 agtaacctat tactacgcca aatgctaatc ctcctaaaac ccataacata aatcttcacc
    23161 tctttatttt actttgaaac acaactacat tacaatcctt tttgttaatt ttttttactt
    23221 tttctttatt tttttgtatt tttcgtttta ttttgtaaat aaattcactt tatttccatt
    23281 attttcctgc acttttatat taattccata tatatcctta agagatttag cgtatgattc
    23341 tggagagttt atagcagcac tcaataaatc tatatctcct atagctttta ttatgaatgg
    23401 ttgtccatat gtagtatcat ttactgtaat agtagctcct gaacatttta tcttactcgt
    23461 tgctagtact ctttccccat ttatagaaat tgctcttgct ccagcctttt ttaaatcatt
    23521 taaaactctc aaaatatcta tatcatgtat aatcaaatca ttcggattct gtccgtcttt
    23581 taactctctc tcactatcct tcatagtaat agttatgcct ggtcctgtaa cacttgaata
    23641 accacttaat tctttaactt cttcaagttc tttttgcata agtgtttgga tagattcatt
    23701 accattcttt tttgcttctt tatattcatt caaagttttt ttgttgtttt taagcgtttc
    23761 ttttaaattt tttatttctt tttttgtatt ttctacctca ctttcaatat tttcagtttg
    23821 acctaatgtt atgtatactc tatttgggtc tagtgatttt atataagttg atatgaaaat
    23881 gcctaatatg aatgcaccta atataactat tttctttttc atactgttat ttcccctctg
    23941 ctttgtttat atcactatga ccatttattt ctatattgtc ttccttggtc acttttactt
    24001 tggcattata atatgtttct atttcccaga caataccata ctttatatta agtgcagaag
    24061 ccaatgtatc agaatcacca attgctttta ttataattgg ttcttttata acagtgtcat
    24121 tcatataaag cttgtcttct tttaaataca tatatgtatt agaaactatt ctctggtcgt
    24181 ttatagatat ggcacttgct tgtgctgaat ttaatttatt tatcattgac aataataaat
    24241 cataattata tagtatacta tcatttttat cagagtttcc taagtctttt gtagaactta
    24301 tctcaatttt tatacctgtt ccttctacat ctgtatatcc agctaatgtt tcatattttt
    24361 ctatttctga cttaagtatg tcttgttcac cttcattatt tttgcctcta taagtctcta
    24421 tatctttctt cacactatta acttctgatt gtagattctc tttttctttt tttaaagatt
    24481 taagttcttc tgtgagttgc tctcctcttt taaaagttgt catccctcta ttttctatat
    24541 taataatttt aagttgtaaa ctaacaagaa cacctaatac gatacttcct atgattatac
    24601 ttttatttat aaatcttttt ttcaatatat cacttcctta caattgtcta tccttctgga
    24661 ctatacactg ccaacttccc ataattaaga tttatagtac ctccattagt atttttattt
    24721 tgcaggtcaa tcaaaatttt acttacccta gatatattat aatctaagtt atcatcatta
    24781 gaaagtaaga tttttatatt acttcttgta agcatttcta tattgctttc tttttttaag
    24841 tttacataat ttatttttct ataaatccct tcttctttta aataatttaa agttttaaat
    24901 acattctcct tagttttata atttttaaat tttatagatt tattatcatc aacactataa
    24961 tctacatcta ctatcaagtc ttttttactt tcattagaac ctcttagttc ttctaacaaa
    25021 ttgccttttt tatctatata acaataatta tcttcatctt ttaatactgc aaatatttct
    25081 ttttctttta aacttataat tatcttattg ggaagttttc tttttatttc cacattttct
    25141 atatatggat tttttataag tttatttttc atatctttaa aattgtatgc aaaaatattt
    25201 tcatttagat ttatatttag ttctttcatt atatttgatt tagttactct cttatttcca
    25261 attacatcta ttttctttac atcaaataaa tcacttttta caattataca tatacaaaaa
    25321 gataacataa gtataaaaac taagactatc atgacattat tcgtatttaa ttttcttctt
    25381 tttttcaaat ataatcaacc cctttggtta ataaatacac ctttagagct tatttttcct
    25441 acaaaaaaat tataatattt aaagtcaaat ttttgcattt tattacataa catccaatta
    25501 aagtattaaa tcttataagt aaatcaatat ttcatcaaaa taaatatagt atgctatata
    25561 tagttaaatc cgtatatata caaataaacc cctaatttag aataatttag aaaatctaat
    25621 atattataaa ttaggggtaa atatttactt atcttttctt tattcctatt actttagctt
    25681 gaccttctat actcggagtc gtttcttttt ttatttcttt tacttcctta ataacttttt
    25741 ctttttttgt ttttttagaa gttgactttt gtgttgaatt gtatactttc attatctcat
    25801 catatataag gtctatggct tcaggcttac ctatagtttt acttgcattt gccatgtcaa
    25861 ccaaaagttc tctatcccct aaaagtttaa atactgctgt atttaggctc tctggagtta
    25921 gatttttttc taaaatagca attcccgcgc cttgtttttc tatactcttg gcattatact
    25981 cttgatgatt ttcagcagta taagctttag gtattattat agatggcttt ccaagagctg
    26041 ttatctcagc taatgaaatt gctcctgcac tacctataac taaatcactt gctgcaagtg
    26101 catttgccat atcttctaag taaggtacaa ccttttgata tggtttaaga tttatatcgc
    26161 taatactttc cataaattca tcataatagc ttttgcctgt agcaaatata aaagctatat
    26221 cttcatttac catattcttt attacaagtc tcattgcatc atttattttt cttgaacctc
    26281 cactaccacc atagcaaagt accattcttt tttcttcact tattgataga ttttttcttg
    26341 caatattttt tcttgataat aaaatttctt ttctaactgg gtttcctgta aaaactaatt
    26401 tttcttctgc tgcctcagga aatcttttat gtgaatcttc aaaacttgta agtacctttg
    26461 taactgtttt agataaaatc ttattagtta caccaggaaa agaattttgc tcatgtatta
    26521 tggctggtat tttccccata gatgcattaa ataaaacagg tccactaaca taaccaccag
    26581 ttcctataac tacatctggc ttaaatttct taacaatctt tcttgattgc tccaaacctt
    26641 taaaaagctt aaaaactctt tttacattat cgaaatctat ttttcgctta aagccttgaa
    26701 ctgttacagt ctttaattca aatccatact taggaactat ttctgactct attccttttt
    26761 ccgtcccaac aaaaattatc tcggcgtctg gatgctcatc tcttattttg ttcgcaatag
    26821 ctatagctgg atacacgtgc cctccagttc caccgcctga taataaaact ttcatcaaat
    26881 catcatctcc tgttaattaa ttttgacatg ctttgagatg tttaacacta ttcctacagc
    26941 tcccataaag atggtgagtg aagttcctcc ataacttata aatggtagtg ctacacctgt
    27001 agctggcatt gatgatgttg caactgctat atttaaagct gcttgtattc caatttgtgc
    27061 acctatacct ataacaacca tacaagcaaa tacattagaa gttttcaatg ctattcttac
    27121 acatctgtag actaaaacta caaacagcat aataactatt atacaaccaa tcaatcctaa
    27181 ctcttctcct attatagcaa atataaagtc attttgcggc tctggtatat aaaaatactt
    27241 ttgtttactc tttcctaagc ctagaccaaa caatccacct gaacctagtg catatagacc
    27301 ttgtattacc tgatacccct taccaagagg gtcttgaaaa gggtctaaaa atgatgtaac
    27361 acgactaagt ctatatggtt cagcaattac taatgcagca aataaagcaa ccccactccc
    27421 tatcatagca aatacaattt tcatatccat tccagcaaca aatatcatca caaaagtaac
    27481 tagaattaca gtacctgctg ttgacaaatt tggctgaagt attatcaacg caaaaaatat
    27541 acctggaact actagaagtg gtacaacacc ttttgtcaaa gattttattt tatcataatt
    27601 tctttcaatt aattttgcag ttaatattat tgtagcaaac tttgcgattt ctgcaggttg
    27661 aaatgttaaa gcccctattc ccaaccatct ttttgctcca tttgcttcta tccctaaagg
    27721 ggtcaaaact aacaataata atactactgc tatagctcca attgctgtag catttttctt
    27781 ccaaaaacta taatctattc tcgatgtaat aatcataact ataaatccaa gcacagcata
    27841 tattacattc ttttttagaa aatagtacgc atcattatgt ttaaatgaag attgtataaa
    27901 acttgcacta aacaccataa ctattcctac aaatactaaa agcatagtag tataaaatac
    27961 tactccatca aattcagtct tcatccttat gtcaatttgt tttttcaaat tttctttagg
    28021 catccaacta atctacctcc attcttccat ttaagtaagt ttagaggtaa gagattattt
    28081 taaattgttt acattatctt tgaagtcttt tcctcttact tcaaaacttt catacatatc
    28141 ccaacttgca caagctggtg aaagaagcac tatatctccg ctttttgaaa tttcataaga
    28201 agtcttaact gcttcttcca tatcttttac tatatgtatg tcattaaatc ctttattttt
    28261 agcacaattt tctatatttg atgcagtttc tcctaataga accaatgatt taacatatga
    28321 ctttgcagtt tcaagaagtt catcaaaatt acttccctta tccatccctc ctgctataag
    28381 tattataggt ctatcgtaag actgtatagc ttttatagta gaatctggat tagttccttt
    28441 tgaatcatta acatatatta cattatctaa atttcttaca aactcttgtc tgtgttccac
    28501 tcctttaaat gtcataagta catcctttat aacctctaag tcaattttgc atacatatgc
    28561 tattgctata gctgccatac agttttctaa gttatgacct ccaggtaaac ttacatcatc
    28621 tttatttaga aatttgattt tatcatctat atctattatt atgtcattat tttctaaata
    28681 tactcctcca tttacttttt tagttcttga aaagtaaatt actttagcat tgcatttatc
    28741 agctagacct tttactatct catcatcata atttaataca cagaaatcct cagaagtttg
    28801 attcataaat atatttgtct ttgcttctat atacttttcc atagtatgat gtctatttaa
    28861 gtggtcttct gtaatattta atatggcact tactttaggt ctaaattcat ctatactttc
    28921 aagctgaaaa ctacttaatt cagttactag cacagattct tcatttgaag tttctatagt
    28981 atctatcaca ggatttccta tattaccaac tacatatgta tctcgttttg ctcttgaaaa
    29041 aatttctcct accaaactag ttgtagttgt ttttccattt gtacctgtaa ttcctataaa
    29101 aattggatta tttgatagtt taaaagctaa ttctacctca ccaattatat acttgcctga
    29161 attttttaat ttcaatatga atggtaggtc tagtggtaca cctggagaca ctacaaccat
    29221 atctatatca tctatatctt ctggatgatg tcctaatatg tattttatat catttataga
    29281 ttttaattca tcaagtatat ttctcaattt attttcatct ttgatatcat ttactattat
    29341 acttgcaccc aatttatcta aatgtttaat tgtagatata ccagtttttg caagtcctac
    29401 taataaaact ttttttcctt ttaaatccat tttttattcc ccccagccct agaatactgc
    29461 tattatactt atccaagcca aaacaactgt aacaatccaa aaaataaata caactcttgt
    29521 ttctggccaa ccacactgtt caaaatggtg gtgtattgga gccatcttaa agaatctctt
    29581 tcttgttgct ttaaaatatc caacttgaat caaaactgat agagcctctg caaaataaat
    29641 tcctccaatt ataggtatta taagcactga gttagttaga actgaaaatg ctacaactgc
    29701 acctcctaat gccattgaac ctgtatcccc cataaacact ctagctggat atgaattaaa
    29761 tcctaaaaaa cctaaacatg ctccaacagt tgctgctgct aaaactgcga cttctgtatt
    29821 tccagcaata gaacttgcaa atagcataaa gaatacagaa actattaatg ttactcctga
    29881 tgctaatcca tctaatccat cagtcaaatt tacagcattt actattgcta caattataaa
    29941 catcattatt ggtatatata atattccaac atttattaca aaatctgtaa atggtatcat
    30001 aagttgtgaa gcacttgatg atgatgtata ttgataaaaa gcaacataaa aagataatgc
    30061 aacttgaagg attatttttt gatatgcttt tagtccaagt gatcttttta gctttatttt
    30121 tataaagtca tctaaaaaac caataaaacc aaaacctgca atacatataa gacctactgc
    30181 catatcatga ctaactttta ctcttgtaag tccagttatt aaaattgcaa caatcattat
    30241 aattccaccc atagtaggtg ttccgttttt agctagatgt gtttgaggtc catcatctct
    30301 aactgtctga ccaaatttaa attttctaag cattggtata aatattggtc ctattataat
    30361 tactattaaa aacgcaatca atgcggtgta cgtaagctct gttattccta acatcataaa
    30421 actcctctcc cttgtcaatt aactctcatt agtttaagct tatttattta gatattcgac
    30481 tattttttct aatctcatgc ctcttgagcc ttttactaaa atagtgtctc ctgtttttac
    30541 taattcattt aacttgttaa atacatcttc tctattctca aagtgatata tattggctga
    30601 attgaatcct aattgttttg cttcttctcc aataaacact gaattttcac ctatagttat
    30661 tataatatca gtattattca tagatgattt tccaactaat ctatgaccat actctgaaat
    30721 ttcacccatt tctagtatat ctccaagtat tgctactctt ctacctttat atcttccaag
    30781 tatctttaaa gcagcatcca tagaatctgg actagcatta tatgaatcat taattattgt
    30841 taatttttca ttttttatta tgtctagtct catttttgtc cctttaaaat ttttcaaacc
    30901 tttttttata taatctaaag aaatatttag acataaacca actaatatag cagacattgc
    30961 attatatata ttatgttctc ctattgttgg tataaatatt tcctcttttt tcccatttat
    31021 tacacatata aatgttaaac tatcctcttc catagtataa ctttcacaat aaatatcatt
    31081 atttttatta aagccaaatg tttttaactt atatacatgt tctttttcct ttaatgtaga
    31141 taaaaactta tcatctccat ttacaattaa agtatttgct tcatcaaagt ttgaagtaat
    31201 ctccatcttt gctttcaaaa tcccctcttg tgaacctaat ttttctacat gtgataatcc
    31261 tatattcgat atgactgcga ttttaggatt tactatatcc actaaatatt ttatttcatt
    31321 gaatccagac atacccattt ctattacagc acactcatgt tctttactta gattaaataa
    31381 agtcaatgga actccaaaat gattatttaa gtttttttca ttttttagta tatttagctt
    31441 tgcagatatt gcagaataaa tcatatccct agtagtagtt tttcccacac tacctgtaac
    31501 acctataaac ggaatatcaa atttttcttt atagtatttt gaaatatttc ctagagccaa
    31561 agatgtatct tttacctcta ttaaatttat atccgaactt tctaatttta ttccattact
    31621 ttcatttttt ataaaagttt tacatccact atcatatgca gacttcataa atgtatgccc
    31681 atctaaattt tctccaacta tggctacaaa tgcattttgg gcatttgcct ccctgctatc
    31741 tataactata tcacttacac agtcattata atctccatat attaacttcc cattagttgc
    31801 aagtactaac tcttttattg ttaagcactc cattatcaaa ctcctccttt aaaaccttag
    31861 ctatattctc ttagcaacaa aaggcaatgt ataccgttat atatacattg cctacgataa
    31921 ctcttaactt tataattata tactatattt ctgttttttt gaagatgttt atgggtatat
    31981 gttaattatc tgttactttt tagttttcaa aatatagggt tatacttgag tcttcattaa
    32041 cttttactcc aggttttggg aatatatcct ttacctttgt atcttctgga agttctatat
    32101 ctgcatctaa gtctggtttt aacttcacat cctccaatgc ctttacagca tctcctattt
    32161 ttagatttct tacatctggc actttaactt gcttcttctc atactcagct ttttcttctt
    32221 ctttgtatac tggtttaact cctaaatatt ttaaagaatc attcattatt tctttcacta
    32281 ttggtcctgc tgtagtacta ccaaatgcgc ttactcctgt tggttcgtca acaatagcaa
    32341 gaactactat ttgtgggtca tcacaaggtg ctattccaac aaatgaacat atatattttc
    32401 ctggagcata cttaccatct ataacttttt gagctgtacc tgttttacca ccaagtctat
    32461 accctggtat atatgctatc tttcctccac cttcagttac aactgattct gcaatttcta
    32521 acattttttt agatgtttct ttagatataa cacttcttac tttttttggt tttactgttt
    32581 cagttatatt tcctttattg tcagtataag attttaccac tcttggttgc attaaatctc
    32641 caccattagc tattgatgat atagctgtta taagttgtat tggagttact gatatagatt
    32701 gaccaaatga tattgtagcc aattcaactg gtcctacatt tttttcgtta tacaatattc
    32761 ccttagcttc tcctggtaaa tctatacctg tcttatccat aagaccaaat gattctatat
    32821 aatcatacat tttcccaaca cctaatctgc ttcctaactc aacaaatact gggttacaag
    32881 aattttgtac tgcttgttta aactcttgtg ttccatgagg tctgtaatgt ctccaacatt
    32941 taatctttct tcctccaaca gttacactac ctgtacatgt gaatttttct ccatctttaa
    33001 ttactccttc ttcaagagca ctggaagatg taataagctt aaatgtagaa cctggttcat
    33061 atgtatcact tactgccgga tttctccaca tttgataata tccttttatt ttatctttat
    33121 cattatattt ttcaagttct tcttgataat atggatatat tggtgttctt gaatcattag
    33181 ggtcataatc tggttttgat gctaaagcaa gtatatcacc agttttaggg ttcatagcta
    33241 ttgcagtaac cttttttgca ttatttaact catacgcttt ctgtactgct ttttcagtat
    33301 agtgttgaat tacttcatct atagacaaca caagaccatt accttgtaca ggctcataat
    33361 atttttccat tccctgtggt atttctcttc ctgaggcatc agtacttact ataagctttc
    33421 caggctttcc ttttaatttt ttatcatatt gcatttctat accagatata cctgttgcat
    33481 ctgaagaagt atgtcccaac acatatggtg caaaattccc atatgggtaa tatctttgat
    33541 tatcttctgc tacccatatt ccacttaatt tagcatctct tattttactt gccttatcat
    33601 catctatcca tctttttact tttacaagag ccatattttt tttacttatt aaagcataaa
    33661 tatctttata atcttcatcc aaaatctcag ctaccttttc ggcggcttcc tttttatctt
    33721 ctacttccac aggtttacac caaacagtat acttagttac acttactgct aactctttca
    33781 tatttctatc atatatagtt cctcttttgg gttctattgg tatatctctt gtttgctgtt
    33841 ctagagcttt tgtacttaac caatttccct tcataagttg tagataccca gtcctaataa
    33901 caaggcaaaa aaataatgcg catgctagaa taagaacgag taccagcctt ttcttactta
    33961 tcctctttac ttttctcaaa tctattcccc ctagttatcc acatctatat actttatttg
    34021 tccatctttt ggatagtcca tattgagcaa ttccttagcc tttagctcaa tttcttttat
    34081 cgatgttttt tcagtttctt ttgcctttag ttcctctaat actatctctt tttttcttaa
    34141 atctttttgt aaataataaa tatcgtactt catttctgag atttttgaat aaccacatag
    34201 attcaatatt attactgcaa aagttgcaat cagtaaaatg catttagttc ctattttttt
    34261 cttatttctc tttttataat tattcttttt tttattttta tattcattta gattatgtat
    34321 tttataattt tttttcaaat tatcacctcg atagtatata gtttaaattt tcctctatac
    34381 tatatttgtt gttaatcata tttcttcctc taaaatttca tcgctaaatt aatacactat
    34441 tgcacaatct aattgtgttt ttttctatac acaacaaaaa cctataaatt cacttatact
    34501 tttatatgaa tctataggtt agatattaat ttttatttat ttttatcact tctaatattt
    34561 tcagataatt tcttccataa atataatatg taactaacta tctactttct aatttctcca
    34621 ttatattact taatatcatc tatatctata tttttatacc taaattttca ttatatatct
    34681 cattagcaaa aaacatttct tatagtgtta attattttgt aattacttta ccagtgcaac
    34741 aaaatatacg atacttttta attaattata acaaatctta ttttatacat aagattactc
    34801 tttaacttta taaattatat tttttctgct actcttagct tagcacttct tgaacgtgga
    34861 tttgcttcta cttcttcctc actaggtaat ataggttttc tagttattat ttttacctca
    34921 gattctttgt cacattggca tataggtaaa tgttgtggac atatgcaatc tgatgctaga
    34981 tacttaaatg catttttcac tattctatct tctaatgaat gaaatgttat tatacatatt
    35041 cttccacctt cattcattat agaagaagca tcatttatca tctttgtaat tactccaagt
    35101 tcattattca cttctattcg tattgcctga aaagttcttt tagctgggtg aggaccatct
    35161 attctggctt ttttagggat agctttttta attatatcta ctaactctcc tgtagtttct
    35221 attgttttgt ttgctctttc ttcaactata aatttagcta tacgtttagc ccagttgtct
    35281 tcaccataat cttttattat cttagttaat tcatcttctg tgtaagtatt tacaacgtca
    35341 tatgcagaaa actcacacct aacatccatt ctcatatcga gtggtgcatc ttgcatataa
    35401 gaaaatcctc tatcagcttc atcaagttga tgagatgaaa ctcctaaatc agctaataca
    35461 ccatcaatct tgtaaactcc tattttttct aactcctctt ttatattttc aaaattgcta
    35521 tgaacaaact tcactctacc ttcatattca ctcaatctct cttttgctgt gtttatagca
    35581 tttttatctt ggtcaaaccc tataaataat cccttatctg aaagtttttt tactatttcc
    35641 ttagaatgtc ctgctcctcc catagtacag tcaacataca ctccatctgg ctttatattt
    35701 aaattctcta tacactcatt taaaagaact gatacatgat gaaattccat agttacctcc
    35761 cttaaattca ctttttaaac tattcttcac taatattgtt tttatttttc tttgataaat
    35821 aaatatgtgc aattagacca ccaataactc caattagact tataacttgt gccattctaa
    35881 gtggacctaa cattaaactg tctgttctta atccctctat aaaaaacctt cctattgaat
    35941 ataaagttat ataagtaact ataacttgac cttcatattt tttatttttt ctaaatagta
    36001 ataaaactat aaatattcca aagtcccata tggattcata caaaaaagtt gggtgtacct
    36061 ttactccatc aaccattatt ccccaaggta aattagtagc acctccatgt gcttctccat
    36121 taataaaatt tccccatctt ccaatagctt gtgctaaggg cattccaagt ataactgtat
    36181 cagccatttt taagaagtta atatttttaa tcttagtgta tatataacca gctaatattc
    36241 caccaattaa ccctccatgt atagcaagtc caccacctct aaaattaaaa atttgagaca
    36301 tattttgagc atagtaactc caattgaata caacataata gattcttgca cagattaatc
    36361 cagctggaat tgctattata gctatattta atacatcatc ttctttaatt cccactcttt
    36421 ttgattcttt tattgctatt agagttccta aaatcatacc acatgccata agtattccat
    36481 accacatgat gtctatgcca aacagtgtaa atgctactct atccataacg tcacctcttt
    36541 ttattaatta aaaatatttc caaagaaata tcctataatt acaattaatt gtaattatac
    36601 taaattatga taacacatta atgttaattc tacaaaaaat attaaaattt attctataga
    36661 tacctatact atattatacc aatctaattg atataaaatt ctttgaactg aacctcacat
    36721 gactaaaatc acaagtgtgc atagatattt tttaatcaaa aagattgatg tatgtataca
    36781 ccaatctttt atatatctta tctataactc ttaaattttt taactcaact tctataaaga
    36841 tatttcataa atttgtttta ctaaaataaa cattcaaaaa cataaactaa catttaacta
    36901 tttattctta ttatgattaa atttctattt aggctctaca atagaaataa cacaatactc
    36961 ctctttaaaa tgttctttta atctttcttc aacttcctca aaagtaatat ctttaataac
    37021 atctaaataa tccaataaat ttatatcctt aaacacataa gatataaaac tatttcctat
    37081 aaagtttata gaatcaaagc acttaataaa tgaacctatt tttttctttt tagttctatc
    37141 aaactcttct tttgataagc cctctttttt tgatttttct atataatcaa gaattatttt
    37201 ttttactttt ttaggttctt tagaatctcc tgctattatg ctaaaagcat aatctacttg
    37261 agatgaaaat ccagctccaa aattgtcatt tattaaacct tgcatatata aatcttcata
    37321 aagcttactt ccttttttaa ataacattcc tacaagtata tcagttacaa tctctttacg
    37381 taaaagttct tttcctttta aaccaacatt actatcttta aaacctatgt tgaacatagg
    37441 catagatata gggaattttt ctatgacctc tttttctttt acactttttg gttcttctgg
    37501 ataaaacctt tctatactct tagagagttt atctactttg taattgtttg attttttagt
    37561 tacatctata actttttcta catctaggtc accaacaaca aatagagcca tatttcctgg
    37621 attataaaaa gtgttgtaac atttatataa ctcttcttta gttattttat atatactatc
    37681 gactgtacct gctatatcaa ttctagcagg ataatttaca tacattgcct ttaaacaatt
    37741 aaagtataca ttccaatcag ggtcatcatt atacattttt atctcttgtg ctattatacc
    37801 tttttccttt tcaacatttt cgtctgtaaa ataaggtgtt tgaacatagt caattagatg
    37861 ttctaaactc tcataaaaat tttcagtagc agaaaataag tatgctgtca ttgtaaaatt
    37921 agtaaatgca tttgcattta ctcctaattt agaaaattta tcaaatgcat ctcctccatc
    37981 aggttgctca aacatcttat gctctagaaa atgtgcaata ccttcattca ctctaatttt
    38041 tttatcttct ccaagtggta caaactctaa atcattcgaa ccataattag tagccaaaat
    38101 ggcatacttt ttcataaaac cccttttagg cataaaatat acatctaatc cattttgcaa
    38161 tttctcataa taaacttctt ctttcaatat atcattaact attttttcca tagacatctc
    38221 ctccattaat ttctcaagaa gtatacagta tcaagttcta tattttttat agcttcaact
    38281 atatcttttt ttgtaacctt ttctatacta gatattatgt cttgtacttc agaatttgtc
    38341 tttgccatag actgagagaa agcaaagtca gacataccac ctatattatc agttatagat
    38401 ttcatagaat taactaaagc aagtttactg ttttctaatt cttcatcaga tatgtcacct
    38461 gcttttacac tttctacttg ttcttttatc aattccacgg ctttatcata atctttagtt
    38521 tctataccag atgatataaa caggatactt ttatacttct ctattgaaga gaatatatag
    38581 taacataaac tttctttttc tcttacatta acaaataatt tagagtgtgg accaccacct
    38641 aatacattgc ttccaactac taaagcataa tatctatctg tatctgcata atccacattt
    38701 gctctataac ccataactaa cttaccttga gttatttcca tttgttcatc tacaaccttt
    38761 acctcatcaa cattttttat aaaatctgct cttggtatat ctataatttc ttctctttca
    38821 aattttagat ttttacttat tatatctaca actttatctt catcaaaatt accttctaca
    38881 actatatcta taggtgatgt tttaagtatg tttttataat gttcatataa ctcttttgaa
    38941 gttatactat ctatttcatc ttcataacca aattcactta tactatatct ctcaccttta
    39001 cacatagcct caatacattt atcttgtgca tattcctttt tatcatttat tattgattgt
    39061 atcttttctc ttaaattctc tttttcaata tttaaatatt cttctttaaa tccgccatct
    39121 acaacaagcg tattatttat cacttcatta aaaaattcta ctacttcttc aaatatactc
    39181 tcatctaaat acttttcact tatgttaatt actttaaaac tcaatacttg acgttctcca
    39241 cgttttacag catcagctcc catagatgaa ccatataaat catctaattt attggatatt
    39301 gctctagcac taggatattt ggcgcttcca cttgctatta ttgatggtat tagagcattt
    39361 tttgtagttt ctttttcatc caatagtctc tgaacatata aacttataag attcgattta
    39421 aacttttctg tttttatcaa agtaagatta atattattac ctaaatttat tattttttta
    39481 gtattaacca tatgtacctc ctatttttat ttaaacatca attttaaaat tgaaatttat
    39541 ttatttttta atttcatata ctcacatata aaaaccttct atttaattat atccttttta
    39601 ttattttcta aaagcaatat ttttatttaa attaaataca aatattattt aaatcaatat
    39661 gatttaaagt ctagtttaca agcttgatat atatgatgta taatcaatat gtatgtctat
    39721 aattaaaaca attttacaat cctatagatt gattgtttaa agttatataa agaatataaa
    39781 aaagaataag agagaggacg attaatatga aattaggtat agttggttta ccaaatgtag
    39841 gtaaaagcac actatttaac gcaataactc aagcaggtgc agaatctgca aattaccctt
    39901 tttgtacaat agacccaaat gtaggagttg tatctgttcc agatgaaaga ttgaacaaac
    39961 tacaagaatt atataattct gaaaaaatag ttcctactgc tatagaattt tgtgatatag
    40021 caggtttagt tagaggtgct tctaaaggag aaggtctagg aaataaattc ttatcacata
    40081 taagagaagt tgatgcaata gttcatgttg ttagatgttt tgaggatgaa aatgtcgtac
    40141 atgtagatgg ttctgtagac ccacttcgcg atatagaaac tataaactta gaattaattt
    40201 tctcggatat agagattctt gaaagaagaa ttaataaaac tcaaaaagct gccaaagctg
    40261 ataaaacttt aggttctgaa cttgatttat taaaatcaat aatgtctact ttagaagaaa
    40321 gtaagtgtgt aagaactatg gaatttacag aagatgaaca aatattcgta aattcattgg
    40381 atttacttac atctaagcct gtaatatatg cttcaaatgt atcagaagat gatttagctg
    40441 ataatggaga aaataataaa tatgttcaac aagttaaagc atttgcagaa acagaagatg
    40501 ctgaagttgt tgtagtatgt gcacaaatag aagcagaaat ttctgaactt gattctgctg
    40561 aagaaaagaa agagttttta gagacattag gtcttgaaca atcagggctt gataaactta
    40621 taaaatcttc ttatgcttta cttggtttaa tttcattctt aactgctggt ccaaaagaag
    40681 ttagagcatg gactataaaa gttggaagta aagctcctca ggcaggagga aaaatacatt
    40741 ctgatataga aagaggattt atcagagcag aaacaatagc ctttaatgat ttagttgagc
    40801 atggtactat ggctgctgct aaagaaaaag ggttagttaa attagaaggt aaagaataca
    40861 tagttaaaga tggagatgta atattattta gatttaatgt ttaagatgat taaacatata
    40921 cattttgact attatatgta tgataattag aaaattaaac ttattgatag attttttatt
    40981 atctctgtat aattatgtaa aattttttaa aagagagata ttctaaatga ttattataaa
    41041 tcatttagaa tatctctctt ttttacatag acggtttcta aataacccac ttttggtcta
    41101 ttctttgcag gaaaaatctt ataagaagcc attccaacaa tatgaccagc cactatatca
    41161 agaaaagcat ctttaggttt tttaatgcca aactccctac caatccaaaa cttaatttac
    41221 caagttctta cacaaacctt cctttgtcat ccgccatttt tctaatttta tacaagttat
    41281 ccctcccaag tttttaaata atttttgata tacttaattg tataaactct acagttaata
    41341 taaagtttta tatcagaata cttcatatac tttaaacaaa tatcatctaa cttgtaactt
    41401 gttattccta acaatttata ttcttaataa tagtacattt ttataaaaaa agtgaacatt
    41461 gattaaacaa agttcacttt ttttaatttt tatatggtat ttcataagtt tatctaattt
    41521 atttcttttc cctttacact gtatctatct aatattaatt tttcatattc ttctattggc
    41581 attggtctag caaacaaaaa cccttgagcc atatcacaac ctataagcct taaaaactct
    41641 gcttgctcca cagtttcaac accttctgaa acaactttta tatcaagttg ttttgccatt
    41701 tgaacaatac ttgaaactac aatttgactc ttttttgcat ttcctactgt tataaagaat
    41761 tgcctatcta atttcaatac atcaattggc atatctttaa gcatatttaa agaagaataa
    41821 ccagaaccaa aatcatccat agaaatttta aatccaacag atttcaaagt ttgcattatt
    41881 ttaaataata tttttgtgtt gtcaaatatt gcactttctg tcaattcaat ctcaattaaa
    41941 tttggctcaa tttcatattc ttttgttatt ctttcaagct cagaaataaa atctggattc
    42001 tcaagatgta ctcttgataa gtttattgat ataggaaatg tggggaaatt ttcattttcc
    42061 caacgtttaa aatttttaca aacttgtgta aatacaaaca tatccaattc tgttataaaa
    42121 ccatttttct caaaaatagg aataaatata cctgggctta ttagaccttt ttttggatgt
    42181 tgccaacgta caagcgcttc agcgcctaca attttagtag ttgataaatc aactttaggt
    42241 tgtaaataaa ctttaaattg ttcatttttt aaagcatcat gcatttcttg ttcaatatca
    42301 cgttcttcta tgagaatttc ttttaaattt tcagcataaa tctcataaac ttttaaatat
    42361 tttcttttta cagcagtttt tgcaatcata gctttgtcaa gacaggtaga aatattggtt
    42421 tctccctctt caacaaaata cacacctata gatggaatta aattgagttc tactcccaaa
    42481 cttgttttta tcattgacaa ttttttacgt ataagttcac atatttctat aattgatgct
    42541 ctattatttt tcttttcaaa tattatagca aagttatcat ttgaaattct tgaaaataca
    42601 gattgctctt taaatatatt cttaattatc tctgctattt cctttaatac tctatctcct
    42661 tcttcgtacc caaacctatc atttactagt ttaaacttat ctatatcaaa ataaattaaa
    42721 actagattct ttttctcatg agaggataaa aattttcccc cttcaagatt aaatttattt
    42781 gaattaccaa tgcctgtaac gttatcttta aaggcaaatt cttctaattt ttccttactc
    42841 tttttatgta taaatagtat ataaattgtt aatgctgtaa agattataat caacactaca
    42901 catgctgata atgtaagttt tataatagcg tatgatttct cagatatagc agctttagga
    42961 acttcagaaa aaagatacca atcattgatt cctattggag cataacttaa ataatatcct
    43021 cttccagaca tactataact taatgaacct gatttaaagt tttttatatt tgacttcata
    43081 ttattgatac tagattttga tattgaatca cttttaccaa tgtaagaaaa taaattttct
    43141 ttagtagagt tggcgttttt actacttgga tttaaaacaa tatcaccatt acttctagct
    43201 acataagaat atccttttcc attaaatgta ggtacagaaa gtattttttc atacaacttc
    43261 gtactatgtg tagcaaatat aactccttta acatcattat ttttataaat tggtacagag
    43321 tatacattaa tatcgctatt ggtattttta tcatcttcta ttacatcttt caactttcct
    43381 gttataacca cttctccttg cattgatttt aagaaataat ctcttttaga gaagtcttct
    43441 ctataaccat ctgtagtgta tgctgtacca tttggtaaaa taacgcccat tcttttaaat
    43501 gaactattaa tagcttgttt tttcagtata gaaaggatat tatctacttc aaaatactct
    43561 tctccttcaa taaatattga aatggactct agtaacttta tatctccatt aatttgtttt
    43621 tttaacacat tgacgctttg atttgctatt tctgaaagat attgattcgt ttcttcacct
    43681 aatgtcttat tcaaccttga accataaatc aaacaggaaa ttgtaattac taagatactt
    43741 atgacagcaa ttatggacaa accataccta ttattttttg catcacttaa cattatgctt
    43801 cctgcctttc tcaaatctat gtatgtataa tcattatctt atgacaatta ttgtaaacat
    43861 tttataataa tacttatttt tccatattta ctgtaattat acaaaattat aaactttatt
    43921 acaacaataa attaattaat gtagatatta gggtttatat tattgcatta tctatttaca
    43981 atcatttaca tataaaaaat gtaatatcaa agtaaaactt gatattacat ttttgttctt
    44041 gtattttgtt tactttttat gctacatttt acttaaacac tcattggcaa cttctttatc
    44101 atcaaagtga tgcttaactt tacctataat ttgataagtt tcatgcccct tacctgttat
    44161 aattacaaca tcatcctttt ttgccatact tatagcttct gatatagctt gttctctatc
    44221 aacaacaaca tggtaatttt cttttttctt gtcaattcct tcaagtatat cttttattat
    44281 tgcttctggt tcttcagttc ttgggttatc agaagttata atgcataaat cagaatattt
    44341 ttgtccaatt gctcccatca aaggtctctt ttcagtatct ctatctccac cacatccaaa
    44401 tacagaaatt atttttcctt ctgcaaattg ttgagttgtt tttagtacat tttcaagtgc
    44461 atcaggagta tgagcatagt ctacaattac acttatacct ttatcatttg gaactgtttc
    44521 aaaccttccg gatactccat tagaaagtct aagaccttct ttatatattg gtttgggtat
    44581 gccaagtaca tagcaagcag ctataactgc taaagtatta tatactgtaa acattcctgg
    44641 aactggtata aatattactt cttcatatga aggtgttatt aatctataag atactccatc
    44701 cgaatctgat tttatatctc tagccataaa atcagcatta gtatcaaccc cataagtgta
    44761 acaaggtaca tttatacctt ttatattttc atatatcttt tttccacctt catcatctat
    44821 gtttattata tttgccatag tagttttaaa aaataatttt tcttttgctt ttctataatc
    44881 ctctaaatct ttatgaaaat ctaaatggtc tggtgttaaa tttgtaaata tgcctagttt
    44941 aaaatctgtt tcatcaactc tatttaatgc caatgaatgt gatgaaactt ccatcgcaca
    45001 gtagtcacat ccattatcca acatattatt aaaatgatat tgtaaatcta tactttctgg
    45061 agttgttgaa ttggaaacaa tgtctttttc tccgtcaaaa atttttatag tacctataag
    45121 cccaacttta ttcttattta aagttaatat ctcatttagt atggtagtta tactagtttt
    45181 tccattagta cctgtaactc ctataacgtt aaatttttga gatggatgat tgtaaaaatt
    45241 atcagctact tttgccatat cttttcttgt atcttttacc tttataaatg tacatccttt
    45301 tatttctaca tcttcttcaa caagaaaggc acttgctccc ttttcaatag catcttttat
    45361 atacttatga ccatctgaca cgaatccttt tatacatata aataatgtac cttttgtaac
    45421 ctttcttgaa tcatattgta cattattaat atctatattt aattcaccct tgacattgat
    45481 tatatccaac ccctgtataa tatcatttaa tttcataaat gctttcctcc ctttaaactc
    45541 tttctgctaa aattacacta catataatat tatattaaga attttttact atgtgaatgg
    45601 ttaaaaatgc ttgaaacatt gcaaaaaatg ctctataatt ttctataata taactgaaga
    45661 aaggtcttct ataaaaacta tggataataa aatcgggtac ttaaatgata attttaaaat
    45721 atttaatatt agagataaaa aaaatataca atttgaatac cataatcatg attttaataa
    45781 gataattata tttataaatg gaaatgtaac ttattttgtt gagggtattc cttataaact
    45841 aaagccttgg gatatattat ttatcagcaa taatgaaatc cataaaccag ttatagattc
    45901 aaatgtcttt tatgagagaa ttgcaatatg gattagccca gaatttatga aaaaaaatag
    45961 tgatgctact agcgacttag aaaagtgttt taaaatatct acagagaata aatgtaatag
    46021 attgcgttta aatatgcatg atattgaatc tataaagagt tttattgagc aaataaatat
    46081 ttctgaatcc aatactgaat ttggaaatga aatattgaga aatgcattat ttatacaact
    46141 tatgatattc ttaaacagaa ttttcctaaa aaacgaaaat atagaatcaa cagaagacgt
    46201 tttttatgat gaaactatta agaaagtatt agactatata aattcaaaca tagatgataa
    46261 actttcaata gatagtatat cgtctgaatt ttttataagc aagtattatc ttatgagaaa
    46321 atttaaatct catactggaa cgtctataca caactatata gttcaaaaaa gattgatttt
    46381 ggcaaagtca ttaataacca atggtcacct tatgagtgaa gtttgtaata aatgtggatt
    46441 caacgattat tctagctttg taagggcgtt taaaaaaaac tatggtgtat ctcctaaaaa
    46501 ttatataaac agtaaaaacc ctttagacat tactctttta gatgaataga taatttatgt
    46561 aaattttatt ttgataaaaa cttgaggtgt tagtatattt atcttactag tacctcaagt
    46621 tttttaattt tttaaaatat atcaaaatat aatgatatat atttctatta cttaaaatat
    46681 atactaacta aattggatgt taacttaacc tatttatgtg ttcattaaat ctcatgtaac
    46741 ttttagttat actatcatat agctatccta ttttttcaat atcgtctctt aatataatta
    46801 ctctattctc ctcttttgct gtagttattc cttttttaat gcttaatttc tgattttgtt
    46861 ctaaattcgt aaatactaca gggcttattg ttgatagacc atttttttca atatattcta
    46921 agtccatttt aactaatttt tctcctgctt ttacttcatc tcctacattc acaaatactt
    46981 caaatccatt tccttctaag tttacagttt ctatccctaa atgtataagt atttctactc
    47041 cacttttttt ggattttatt attattgcat gcttagttgg aaatataact ccaatagttc
    47101 catcaactgg ggaataaatc aacccatcat tagatttaat tgcaaatcca tcccccatca
    47161 atttacttga aaatacagaa tctggcactt cttctatatc taatatctct ccatttgtta
    47221 atgaataaat attttctact aaagcatttt ccatattggc aacttccata ttttttttca
    47281 ttttattttt atcgttattt ttaataacag cactttcaat ctttgttttt ccctctatta
    47341 tatcttttat atcagacttt attgaatccg attttggtcc aaatataact tgcacattgt
    47401 tgccaataac cataacacct gctgctccaa gtgatttcaa tttttcttta tctactttat
    47461 ttatatcatt tacaatcaat ctaagtcttg ttatacacgc atctagactc tttaagtttt
    47521 ctttatttcc taaagcaacc aaaatctctg atgctaattc attcttagac atatcaatga
    47581 cttcaacatt agtttctgaa tcatcttctc ttcctggtgt ctttaaatta aactttctta
    47641 tcataaatct aaatacacta tagtataata ctgcaaaacc aatacctacc aaaatagcca
    47701 agtaccatgg agttctatta ggaagaacac caaataataa gaaatctatt aatcctcctg
    47761 aaaatgtgaa tccaatttta actcctaata attgcataac tgcgtatgat aaaccagaaa
    47821 ataaacaatg tattgcaaat aatactggtg caacaaataa aaacgcaaac tctattggct
    47881 cagttattcc cgttaaaaaa gatgttaatg ctgctgatgc cattatacct gctacaaatt
    47941 ttttcttttc tggtttcgct tcatgcacca tagcaagagc tgctgctgga agaccaaaca
    48001 tcataaatgg aaatgcacct gtcataaaag tacctgctgt gaaatttacg ccatctttaa
    48061 gttgagcaaa gaatatcttt tggtcaccca ttacaagctc accagcttta tttacatatt
    48121 ctccaaattg ataccaaaat ggattatgga aaacatgatg caaaccaaat ggtattaata
    48181 atctttctaa agaaccttcc ataaatgctg ctagtgtttg atttgcttct ataacatttt
    48241 ttgagaaact aagtaatccc tcttgtattg gaggccaaat aaaagacaat ataattccta
    48301 ttgctatagc aactaatgaa gtaacgattg gtacaaatct tttacctgca aaaaaaccta
    48361 gatactgagg taattcaatt ttataaaact ttttataaag ataagcagcc actataccta
    48421 taataatacc accaaatact cctgtttgaa ttgttggtat tccaacaata cttgcataca
    48481 tagaattatc tatcatatct tctgtaacac ctgccataac acccatagat gtattaagaa
    48541 ttaaaaaacc tactatagct gccaatgctg caacaccttc tccttcggtc aaaccaacag
    48601 ctacaccaac tgcaaatata agtggtaagt tattaaatat aatttgacct gaactttcca
    48661 ttatacttat tataagttgg aatgtagggt tcgctaaagc tggaaaatga gccaacaacg
    48721 catctcctgc taaagcatta ctcacgccaa gtaatatacc tgctgcagga agtaatgcaa
    48781 ctggtagcat tagagacttt cctacttttt gtaaaacacc aaatactttt ttcatttact
    48841 tttctctcct tattatttta tattttttaa ataaaaaaga cttaagttta taaaaagtta
    48901 cattagtata cttaattcta aaa
//
LOCUS       DAMJVM010000020        48408 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.020, whole genome shotgun sequence.
ACCESSION   DAMJVM010000020 DAMJVM010000000
VERSION     DAMJVM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 48408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48408
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.020"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            77..379
                     /locus_tag="PC523_001228"
     CDS             77..379
                     /locus_tag="PC523_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF2474772.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGVNSKYTRAKGFK
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPNILINSINNKTDYIIGKEIE"
     regulatory      481..573
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            673..1467
                     /gene="thiD"
                     /locus_tag="PC523_001229"
     CDS             673..1467
                     /gene="thiD"
                     /locus_tag="PC523_001229"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDF2474773.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTQGVFAVEDLNKNIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVKNLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGKEILQLGPKFVLMKGGHLDGEAVDILIGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNFSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            1491..2276
                     /gene="thiM"
                     /locus_tag="PC523_001230"
     CDS             1491..2276
                     /gene="thiM"
                     /locus_tag="PC523_001230"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDF2474774.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANATLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEDSTDIIRKVAKKLECIVVA
                     TGKIDIITNGKSTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLTM
                     SLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            2299..2931
                     /gene="thiE"
                     /locus_tag="PC523_001231"
     CDS             2299..2931
                     /gene="thiE"
                     /locus_tag="PC523_001231"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDF2474775.1"
                     /translation="MIDKESLKKCLRLYLITDSEMLEGRDFYKCIEDAISSGITTVQL
                     REKNTCGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNDKILGVSARTLEEAICAKNDGADYLGIGSIFSTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLDSDGYAIISAILKAEDISKEVEKWTLKI"
     gene            3070..3372
                     /locus_tag="PC523_001232"
     CDS             3070..3372
                     /locus_tag="PC523_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2474776.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMFDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(3438..4187)
                     /locus_tag="PC523_001233"
     CDS             complement(3438..4187)
                     /locus_tag="PC523_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2474777.1"
                     /translation="MLNILWRSMKWRFKNPISFIVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIILVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVAILCIVSIFFSVTIESGIGGILLMTVLVFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIENLSGGLKVVVMLNPFTHVINALRSLIFGETVLI
                     EDILPVILLFLILCCASFSLAMWRLKKEMVS"
     gene            complement(4181..5143)
                     /locus_tag="PC523_001234"
     CDS             complement(4181..5143)
                     /locus_tag="PC523_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2474778.1"
                     /translation="MLAVEIDNLVKEYKNGVKALNGLSFNVNSGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMIFQS
                     KMYKVKPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRYEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNIIRITFHNTEDIKKYNDAIKGTGLVKFMNVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEGWEC"
     gene            complement(5147..5599)
                     /locus_tag="PC523_001235"
     CDS             complement(5147..5599)
                     /locus_tag="PC523_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2474779.1"
                     /translation="MSYKIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNGAP
                     FFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHIGSYSNLPKTYE
                     MIFKYIHENNIKIQTPWREVFIKGPGMLVKGKPDNYITEIIFPLKEEE"
     gene            5960..6316
                     /locus_tag="PC523_001236"
     CDS             5960..6316
                     /locus_tag="PC523_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2474780.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVIGELKPGDTIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNHQSSVTIDENILKNLKLELINESLSVFLKDMKA
                     INVTKEEVLKIIEDKY"
     gene            6388..7104
                     /locus_tag="PC523_001237"
     CDS             6388..7104
                     /locus_tag="PC523_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2474781.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINTDSGEIRIDGEKINEKIYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKKKSKEMMNIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVIILDEGQIIASFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            7097..7921
                     /locus_tag="PC523_001238"
     CDS             7097..7921
                     /locus_tag="PC523_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2474782.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLGLNLFEINKFKNS
                     KFIINLAIGDFGGIFYGIGILSNLGFIQSILFFGMLGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALLTMIYGFLISQIATLFICKYIFNFIFRNVPIINMNFAK
                     DLYYLNLKGIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYILLITLLIIVG
                     LYFSTIYISIITLFRKYYMAIGFQFNNLVAGLLSFLIIMFILNGISYLLIKKKISL"
     gene            7932..9131
                     /locus_tag="PC523_001239"
     CDS             7932..9131
                     /locus_tag="PC523_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474783.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIGLLNNEDNLKIKTIKGDPNELD
                     YININFGYAFNGYVNSYYTINGSNKTKKTELIKLQKDAKNTNNLIDVSSGSDLIAHVG
                     FEMNDDSKDINFVIDEKKEDLEKGVFPSEGDDKAVEYEKLDMNDFTRTRYKVDKSSDN
                     YYANILSSKRYKKYLYVSVLYATSDSSKNNKEYSSTIDIIKINPENKKIQTIKSINLS
                     NYFSKKYQNIDNISVSYASKYEDKMYILVNVLNANTGDKSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKVKLEDNKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYILDN
                     KEKRGIRSIEIDSNKIIVYTSRLNSTLSWDNKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            9140..9952
                     /locus_tag="PC523_001240"
     CDS             9140..9952
                     /locus_tag="PC523_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474784.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNAIGFSSFRNIMGYTESWFMFGIIVCICYAFFIWYRDF
                     NGRSKSIYTLIMIPKNRINIYISKLLNILFLVYSYTVVLTISLFITSKLLPRHMLGNV
                     TSYGFVQETIYNLKMLLPYSFEILFVQYIFLLIGFVSVVFTSILINKSIYRVSALISF
                     LFLVLEILVFIISIEFIIPYEYSNVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            9963..11231
                     /locus_tag="PC523_001241"
     CDS             9963..11231
                     /locus_tag="PC523_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474785.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYDEVIISKNGVRNNTNPTSIIRNDYLNIDKKYSDFVNDKG
                     KDLIYKDDLNIGEAYTSVKYDLVQGMNALNDCIKVSNKNLKSGAEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISSSKVNNICILKIDIKNKSYKLLNRFNLVLQNG
                     YQQNINEIKFSIGSKLYLEIVNNQSTNEDIEEHDNYSNFIIYDIEENTFNISDKFMDN
                     LNKEKNANPKEIGLLGKTKLDYMVKNNKLNLIVTDMSNSITNLVYDINSNKIKLEDYE
                     ILDLKVDYNEASSGIRKVKLLDDKIYSISGIKNNLSGELKGFKGVVDIVGTNPVKFKV
                     FDMKLKKTVYEAELINGDVSLQEKLFFVAN"
     gene            11588..12769
                     /locus_tag="PC523_001242"
     CDS             11588..12769
                     /locus_tag="PC523_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2474786.1"
                     /translation="MNKKYIKAGNLIDGTGNSTMQNKGIIIDGDTIVEIEDIREDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKVYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVDGNPIENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(12911..13828)
                     /locus_tag="PC523_001243"
     CDS             complement(12911..13828)
                     /locus_tag="PC523_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474787.1"
                     /translation="MENNKCREDFRFTQEYEEDYMNANDRYYENYQVADRYYNYPNKY
                     KEPKMTKCCCKKSMREALELLRYDSLRPFVNFNQFAFISDFFIVGAYLVGIDYSIPPK
                     DNLSGLNGTFERFSACNCDLIDISGQLYYPKPVPLTLGGLVTSIGTIPGVSELVALIE
                     AAVPATIDLGSILNSILDYIIDYIISTVNPLTNVDLASLCNLKAVAFNVTPADYEDFI
                     SSLAYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVLVLGNSNDSRIYFVCVDSIDYIA"
     gene            14081..14875
                     /locus_tag="PC523_001244"
     CDS             14081..14875
                     /locus_tag="PC523_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2474788.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKIKTKKEEFFAVNNVS
                     FKVIEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINVSGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEEIEESIENVVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGEIIASGTPQSLKEESGVNNLRDVFIKLARREDI"
     gene            14876..16936
                     /locus_tag="PC523_001245"
     CDS             14876..16936
                     /locus_tag="PC523_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2474789.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKNIKNTSSDKGKIRVLDVDNYK
                     KSLEENKIDAYIKIEEKKSSTSYRIYINSSQENSSNAVKRIETVLDKYKRSTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMTFILVSGGISNQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGIVGIIMTQFIIISLPLLFAYYIKADFKEVFSLKIPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQSMEVAENLNKALFMKDSVLLNLLVIALLPAICEEIFFRGFIFSSF
                     SKSKDKNKSIKLTIICSSVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNGVAVMLNHYTTGNIINLYKFVEVDFSNLNVLEFALILLISLILVMLGARALGRK
                     SLRN"
     gene            17543..19087
                     /locus_tag="PC523_001246"
     CDS             17543..19087
                     /locus_tag="PC523_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2474790.1"
                     /translation="MRNFKKKLMFFLFICIILQITCTTSVYSLCKIYKNNSVNNFEFI
                     VSNNQLISKYALQMILIMATICIILIFYIIYDKLNFKIKLQKIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEM
                     FARISADNFIIIMKYIEEEDITNRLKIIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFEKNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTISVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVVEMAKELGMKVISEGVETEEQFELLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLIRG"
     gene            19263..19631
                     /locus_tag="PC523_001247"
     CDS             19263..19631
                     /locus_tag="PC523_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2474791.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEILKLITENLKGEL"
     gene            19633..20358
                     /locus_tag="PC523_001248"
     CDS             19633..20358
                     /locus_tag="PC523_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2474792.1"
                     /translation="MDKNINNISQDGIVEFKGVNKSYGIKTVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIETKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEVIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            20352..21116
                     /locus_tag="PC523_001249"
     CDS             20352..21116
                     /locus_tag="PC523_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2474793.1"
                     /translation="MLAKLLKYELKASGRVFIPLYIAILIVAVFNGIFMNTNILQIQG
                     IAILVLTSLFMALAVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVVSFIVAIFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILVLLLVMLTSYS
                     LFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPKENTNMIYH
                     FYQSSHVMFAILGDIVVIVALFFATKFILDKKLNLE"
     gene            21362..22612
                     /locus_tag="PC523_001250"
     CDS             21362..22612
                     /locus_tag="PC523_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2474794.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLENELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKVQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTELYEESEKVNIVDGIKSVIK
                     NKSTWYNSMIMLSFVGLTSAFISLWGVRYIMDVYCVSKSFSAFIVSFFTYGFIFGSLI
                     MDFVFAKIRSSKFNIIKYGAIIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNVVDGYKKSFVV
                     FIVLSIITIVSSHIGVKNDDFKAL"
     gene            22804..23688
                     /locus_tag="PC523_001251"
     CDS             22804..23688
                     /locus_tag="PC523_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDF2474795.1"
                     /translation="MDTTKIDDISKLDLKNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRRFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYIEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFAARVWCNTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            24047..24664
                     /locus_tag="PC523_001252"
     CDS             24047..24664
                     /locus_tag="PC523_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDF2474796.1"
                     /translation="MCKKTYLLVVSILIAGLLTACSGSPTADSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQNFKVPYTDAINTFKDKYKDADVVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTENLDSEDLNGVARKEKLDLNDIITPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKIDSKKGTVLKVEKED"
     gene            24770..27301
                     /locus_tag="PC523_001253"
     CDS             24770..27301
                     /locus_tag="PC523_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HDF2474797.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSDYKVNLFDVIEHKQEDILAKISVSQGVLFGTPTILGDALKPIWDILVSL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMKAVKPFAVNFKPSNEEIDKLNSY
                     TGKFLDKLNATFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEIEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKAWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVI
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVIFKDGDSVDC
                     DMVVFSIGVRANTQMAHDTSVKIDRGIVVDKTLQTTVKDIYACGDVAQVENTSLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDDSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            27512..28132
                     /locus_tag="PC523_001254"
     CDS             27512..28132
                     /locus_tag="PC523_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDF2474798.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFVGCLLSWIGSGLGTASLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDVKDFVPAMLAGKFVMFIVISYIASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHQSDKDDKMI"
     gene            complement(28299..28835)
                     /locus_tag="PC523_001255"
     CDS             complement(28299..28835)
                     /locus_tag="PC523_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF2474799.1"
                     /translation="MIKETHERGGYILALLILPFINNLYLVKYDIAYKIVLIIVYIYF
                     AYLGSLFPDIDMRGSYISKKFTLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKLISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(28948..29622)
                     /locus_tag="PC523_001256"
     CDS             complement(28948..29622)
                     /locus_tag="PC523_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDF2474800.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNVALEKYPELYNYSLCELLQNLDSLPKDIATAVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKAALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNAVNWNGALENYK
                     NLKSQD"
     gene            29825..31708
                     /gene="asnB"
                     /locus_tag="PC523_001257"
     CDS             29825..31708
                     /gene="asnB"
                     /locus_tag="PC523_001257"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF2474801.1"
                     /translation="MDGNISIYYKGDISKKENIKFKIKESLNNINNTRVSSRIEYKSE
                     AKIFDFERFNVIDVIYGNKPFEKYGKVIVFNGNIYNNHEIKEELIKRGHSFTTGDDIE
                     VLLASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKESAIIFSS
                     EYKFILEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKR
                     YNKLEFRSSTKFFYTKNHLGNRDVDKEEVRNIVVDSIRNHMEEHKEIGTFLSGGIDSS
                     IITTVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKALPN
                     VIYSLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVN
                     SPTYIKGILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEK
                     NIYEYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFL
                     YKDVFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSL
                     GGIVKETISNSNVDEFIDKKYVIKLLDMHLKGHRDNSRKIWTIFTFCLWHQLFIEHKN
                     VEY"
     gene            complement(31799..33235)
                     /locus_tag="PC523_001258"
     CDS             complement(31799..33235)
                     /locus_tag="PC523_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF2474802.1"
                     /translation="MICTIKPLKISIKKSNEPLIEFMSNTTYQNKSSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPIIPYIRLSSLNINFDKEQTDKMLKIFNIYDSAK
                     FNSWDLYNINFPCKIENDTLNWTKKIAFKNTLDLFSMSMPNCTPSIIKNFAVKLLCWI
                     EYFLPKLFYNKVNTNISPKILYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSNIFELSRKTPNILEIPFERHIANINYNENTNKTSHPMNKPNSENTFNNTNLI
                     SKKIVQGEEVELSYEELAKLSASVVMIVVCNKENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSGYDLAVIKVDRKCKPIPVKIGKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESSLVKIFANNFIEIVN"
     gene            33564..34142
                     /locus_tag="PC523_001259"
     CDS             33564..34142
                     /locus_tag="PC523_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2474803.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            34181..34759
                     /locus_tag="PC523_001260"
     CDS             34181..34759
                     /locus_tag="PC523_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2474804.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLEAMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            34845..35474
                     /locus_tag="PC523_001261"
     CDS             34845..35474
                     /locus_tag="PC523_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2474805.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGAGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            35464..38223
                     /locus_tag="PC523_001262"
     CDS             35464..38223
                     /locus_tag="PC523_001262"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF2474806.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMVSKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     LNSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQARKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKEE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            38310..39872
                     /locus_tag="PC523_001263"
     CDS             38310..39872
                     /locus_tag="PC523_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HDF2474807.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKNGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDKVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNVEFEEANIIEEKTSFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFNFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGVIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIIFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            39873..41009
                     /locus_tag="PC523_001264"
     CDS             39873..41009
                     /locus_tag="PC523_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF2474808.1"
                     /translation="MGININSFSKDKNSVDVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSSQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            41071..42147
                     /locus_tag="PC523_001265"
     CDS             41071..42147
                     /locus_tag="PC523_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HDF2474809.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHSITGILIPKANSELIENCIEALDSMNLQDMYVI
                     PIIETREFIYNEKKELSFINLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMKNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNEINPHFLWAK
                     KTLILSKIYGVLNEGVDYEELLKF"
     gene            42125..43363
                     /locus_tag="PC523_001266"
     CDS             42125..43363
                     /locus_tag="PC523_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HDF2474810.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDIGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLIATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYTITKLDTILSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITIDNKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQCDVYYHST
                     TRSPIINIDKHNYPIRSKFMLKSFYNMDIQNYIYNIDKQNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            43417..44505
                     /locus_tag="PC523_001267"
     CDS             43417..44505
                     /locus_tag="PC523_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HDF2474811.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYVKSKYNINLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNDKFKKHFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFVL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDV"
     gene            44533..45324
                     /locus_tag="PC523_001268"
     CDS             44533..45324
                     /locus_tag="PC523_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HDF2474812.1"
                     /translation="MIVFSDLDRSIIYSNKFLSADSKYANIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKSDYEPIESMIYKFKDYLNVDGITNFKVAEDTFFYIVVDFSKFNLESIKEYTNV
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAIGDSIMDYGMLNISNK
                     AYLLKHGDINKNEIENSFLSSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(45439..45993)
                     /locus_tag="PC523_001269"
     CDS             complement(45439..45993)
                     /locus_tag="PC523_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2474813.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDDVCISVWAMTPPSF"
     gene            46187..47389
                     /locus_tag="PC523_001270"
     CDS             46187..47389
                     /locus_tag="PC523_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HDF2474814.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGVTEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            47446..>48408
                     /locus_tag="PC523_001271"
     CDS             47446..>48408
                     /locus_tag="PC523_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HDF2474815.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISEHKINILIGLE
                     GLSHIGSNIDSIDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVHKME
                     NLGMIVDVSHLNEKSFWDLMSIASKPVIASHSNAKSLCDVKRNLTDNQLKEIANTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NSSMPSVDGLESTHTAHNIVVELENRGYKKRDIDLISSGNFYRLF"
BASE COUNT        19006 a         4973 c         8112 g        16317 t
ORIGIN      
        1 atattcaaaa tacacaccat tttttttaat tctataatat aatatttata tatttaatgt
       61 taggatgtga tttaagatgt attttacata tattataaga tgtaaagatg attctctata
      121 cactggatat accagtaata tagttagaag aatgaatgaa cataagttag gagttaattc
      181 aaaatacaca agagcaaaag ggtttaaaaa actagaagtt tattttgtga ctaatacaaa
      241 aagtaatgct atgaagttag aatactatat aaaaaaatta actagaaata aaaaattgtc
      301 tataataaaa aatccaaata tacttataaa ttcaattaat aataaaactg attatattat
      361 tgggaaagaa atagaataat taacataaac attcatagta acattataat gagtacatat
      421 agtgtaaaaa cttgacaata aatactattg taatataatg aagttatgaa tatagtaaat
      481 taaaatagct aggggagcca agagctgaga ggtataccga cccttacacc tgatctggat
      541 aataccagcg taggaaagct tagtaaaaat tcaagtatgt attatttgtg atatatgcta
      601 tttctaagct atacctattg taggtatagc ttttttaatt aagttattta aattaaactt
      661 taggaggcaa taatgagtaa ttataaaata ccaacactta caatagccgg ttctgactca
      721 tctggaggag ctgggattca ggcagatttg aaaaccttta gtgcaattgg tacttatggc
      781 atgagtgtaa taacagctat aacagcacaa aatactcaag gagtttttgc tgtagaggac
      841 ttaaataaga atataataaa aaaacaaata gaggcagtct ttgaagatat acctccaaga
      901 gctgtaaaaa taggaatggt ttcaagtcct gagattatac ttgaaatagt taagaatcta
      961 aagaaataca atccaaaata tttggtggta gacccagtaa tgatatctaa aagtggatat
     1021 tatctattaa agccagaggc aaaagaaaac ttaattaagt atttgatacc acttgcatac
     1081 ataattacac caaatatacc agaagcagaa gaaatcacag gaataaaaat acataatgta
     1141 gatgatatga agagagtagg aaaagaaata ctgcaacttg ggcctaaatt tgtacttatg
     1201 aaaggaggtc atttagatgg agaagcggta gatattttaa ttggtaaaaa tatatttaaa
     1261 gtttataaaa gtgaaagaat tgataaaaaa aatacacatg gaacaggatg cacattatct
     1321 tctgcaataa catcttatct agcattagga tatgaaataa cagaagcagt aaattttagc
     1381 aaaatctata taacagaagc aataaaaaga agctttgata taggtcatgg agtaggacca
     1441 gtacatcatt tttataaatt tgaataataa aaaaatattg gaggtagaat atgtataatt
     1501 taattaaaga cgtaaaaaaa ttaaatccat tggtaattca ttatactaat aatgtaacta
     1561 taaatgattg tgctaatgca actttagctg taggagcaag tcctcttatg agtttttcat
     1621 atgaagaagt agaagaaatg gtaagtgtag caaactctgt agtaataaat ataggaacta
     1681 tgaactcaaa tatgttggat ttgttcctct tagctggaaa agcagcaaat aagtacaata
     1741 aacccgttgt tttggaccca gttggagtat ttgcaagcaa agcgagagca gaacttacaa
     1801 gtagactttt aaatgaagtc aaatttagtg ttgttaaagg gaatgtgtca gaaataaagt
     1861 ttataggtgg ttttaatgta aggggtaaag gcgtagattc atttgatgaa gaagattcta
     1921 ctgatataat aagaaaagtt gctaaaaagc ttgaatgtat agttgtagca actgggaaaa
     1981 tagatataat aactaatggt aaaagtacat acaaaataaa caatggaact gataaactga
     2041 aaggaattac aggcactgga tgtatgactg ctagcttaat tgcaagtttt atggctgtta
     2101 ctgagaatat attagaagca gcaacaatgg gtgttcttac aatgtcttta agtggcgaat
     2161 tggctaattt aaataatccg ccaataggta catttaaaga aaatcttatg aatgctattt
     2221 atcaaatgga catagatact ttaagtaaaa attctaatat tgaattttta aattaataaa
     2281 gagtaaggga gacttaaaat gatagataaa gaatctttaa aaaaatgctt aagattgtat
     2341 ttaataactg actctgaaat gcttgaaggt agagactttt ataaatgtat tgaagatgca
     2401 atatcttctg gaataacaac tgtacagctt agagaaaaaa atacttgtgg aagagaattt
     2461 ttaagaaaag ctatgaaact tagagaaata actaaaagat atggagttaa gtttataata
     2521 aatgatagag tggatatagc acttatttgt gatgcagatg gggtacatgt aggacagagt
     2581 gatattgatg tgagagaggt tagaaaactt ataggaaacg ataaaattct aggagtatca
     2641 gcaagaactt tagaagaagc tatttgtgca aaaaatgatg gtgcagacta tttaggcata
     2701 gggtctatat tctcaacatc tactaaatta gatgcaaaat cagcctcatt tgaaactgtt
     2761 aaagaaatta aagaaaaagt agacatgcca tttgtactaa ttggaggtat aaatttagac
     2821 aatatagata aacttaaatg tttagatagt gacgggtatg ctataatatc ggctatacta
     2881 aaagctgaag atatatctaa agaagtagaa aagtggacat taaaaatata aaattggctc
     2941 tagtagtgaa aaaaatggaa aaatttgata taatctaaga gtaaaattta agaaattata
     3001 aatgataaac ttttctacat atacttttta atcaagatat atgtagattt ggttaaaaag
     3061 gagttataaa tgattaggat attgttagta tgtgttggag gcatgtcttc cactctattg
     3121 gtaaataaaa tggagaagga cgcaaaaaag agaaatatag attgtaaaat atgggcagtt
     3181 ggagaaggag atataaaatc agagcttgat aacttcgata ttttacttct tggaccacaa
     3241 cttagattta tgtttgatga tgtaaagtct atagttggag atagagctcc agtgtctata
     3301 atagatatgg taaactatgg tacttgtaat ggtcatgctg tgttaaattc ggttttagaa
     3361 atattaaaat aaataataaa agtggattct atataataga gtccactttt attatttgtt
     3421 attaatggat ttaaaactta gcttaccatt tcttttttta atctccacat agcaagtgaa
     3481 aaacttgcac agcacaatat taaaaatagt aggataacag gtagtatatc ttcaataaga
     3541 acagtctcac caaatattaa gctacgtaaa gcgttaataa catgggtaaa tggattgagc
     3601 atcactacca cttttaatcc tccagataaa ttttctatag ggaaaagagc tgaacttaaa
     3661 aagaaaatag gaagtacaat tgcagtcata attgtttcat aaactatctc atttggcaac
     3721 aataaactta tagaataagc tagacttgat aaaaagaagg cggtcataaa tacaagcact
     3781 gtcataagta ggattccacc aatgccagat tctatcgtta ctgaaaagaa tatacttact
     3841 atacacaaaa tagctacttc tatgaaggaa actaagatag cttctaataa ttgaccaagg
     3901 acgattgaat acctactgat aggtgcaatt aagactctat agaaacttcc actattcttc
     3961 attataaagt tgatgatacc accactacta caagaagaaa acacaaccag tataattata
     4021 cctggtagta taaaagcagt ataattattg atatttatat tattcatagt ttggtttgca
     4081 atagaactat aaagcacaag ccaaagaaaa ggttgtaata tagtaactat aaatgagatt
     4141 ggatttttaa aacgccactt catactacgc cataagatat ttaacattcc catccctcct
     4201 ttaggctttt tttagaactt gtaagtgcta aaaatacatc ttcgagactt ggttccacaa
     4261 tttctattgc atcaaattct atatcatgtt ctaaaagcca tttatttatt aaagtaaaag
     4321 cagttctact atcgtttaca cttataaaaa ttgaattttc tcgtacattc ataaacttta
     4381 ctaaaccagt acccttaatt gcatcattat atttttttat atcttcagta ttatggaaag
     4441 ttatacgtat tatattttgt ctaatgtaac tacgtaaact ggatggtgtt ccttgtgcta
     4501 aatcttttcc attcttcata atacaaatgt tatcacttaa ttgttcagct tcttcaagat
     4561 aatgagtagt tagaaatata gttgtgccat actcatatct aatttttaat aacatatccc
     4621 acatagactt tcttgaatct acatccatac cgacagttgg ttcatctaga aatagaattt
     4681 taggagatga taccatattc atagctatgt ccaatctacg ttttacacca cctgaatatg
     4741 atgaagtagg atattttaag taactagata agtcaaactt atcaattaaa gagtctattc
     4801 tttgttttgc aatctgtggt tttactttat acatcttgct ttgaaatatc atattttcca
     4861 taagagataa gtgttcatca atagagattt gttgtgctac acaagcaatt tgagtacgta
     4921 tccaagaagg attatctact aaatcttttc caaacattgt tacatttcca gatgtaggct
     4981 tgtaaaaagt agtcaaaata ttgataagag aagatttacc agcaccatta gggccgagca
     5041 aagaaaaaat ttctccagaa tttacattaa agcttagacc atttaaagct ttcacaccat
     5101 ttttatattc ttttactaaa ttgtctattt caactgctaa catatattat tcctcctctt
     5161 taagtggaaa tattatctct gtaatatagt tatctggttt tccttttacc aacatacctg
     5221 gtcctttaat aaatacttct ctccaaggag tttgtatttt tatattattt tcatgaatat
     5281 atttaaatat catttcatac gtttttggca aattactata agaacctata tgagtaaaaa
     5341 acaaagcttt tgttttagga aattcttttg tagttatacc catgcgattt ggagtctcag
     5401 cagttggaac acataattcc atttctgcaa caccagtttt ttggtcaaca tcatagtagc
     5461 aaaagaatgg tgcaccgtta gactttcctt ttatagcttt aaatacattt ggaaaatatt
     5521 ttgttgcctc agtcataggt ccattataac gcatggtagc tactgtgact gattcaatat
     5581 ttttgatttt ataactcatt tgtttacctc ctaatgtgta ttcatatttt tattagattt
     5641 ttaagcatca atacatataa gattttgatt agtagaaagc atataatctt atctcgtttg
     5701 cttgtgttca agtctaacat aacataaaat atatatcctc tcatttgatg ctccattttg
     5761 taaagttatt atttaagctt tattttagtt ttttagtaaa tttaaattaa aataaaataa
     5821 tgtataaaaa aagtgtttac aaactaagtg tattaatcgt ataatacact taataggata
     5881 ctagataagg tctgaaataa agacatattt ttttaaagta agtgtactat cgtattaata
     5941 cactagaata ggaggaacca tgaactttga attagataat acaactccca tttatttgca
     6001 aatagtaaag tatataaaga gacaaatagt aataggagag ttgaaaccag gggatacaat
     6061 accatcaaga agagaaatgg cacttaatct aaaagtaaat ttaaatacgg ttcaaagagc
     6121 atataaggag atgggggata tgaatataat aaatactttt aaaaatcatc aaagcagtgt
     6181 aacaattgat gaaaatatat taaaaaattt aaaattagag cttataaatg aatcattatc
     6241 tgttttttta aaagatatga aagcaataaa tgttaccaaa gaagaagttt taaagataat
     6301 agaagataag tattagtttt ttaacatata gaaaacttag tttagaaagt ttatttataa
     6361 gcatattgga tgtggaggat agtaaatatg atagaaataa aaaatgtcag caaaacatat
     6421 aaaaggatgc aagggcttaa aataagaaag atagaggctt taaaaaatgt aagttttaat
     6481 atagaaaagg gaaaaataac tgcattactt ggtataaatg gcgttggtaa atcaactatg
     6541 ctaaaagcaa tagcagggct tataaacact gatagtggag agattcgtat agatggagaa
     6601 aaaataaatg aaaagatata caataaattg gcttttgttc cagatgtaca atctcacttc
     6661 agtaatacaa caataaaaga aacatttgaa tttatggaaa ttttttattc aaagtggaat
     6721 aaaaaaaaat caaaagaaat gatgaacata tttaaattgg atgaagatga aataattgat
     6781 aacttgtcaa aaggaaacat tgcaagagta aaattaatac ttggattttg ccaagaccca
     6841 gagtacatac ttcttgacga accatttaca ggaatcgatt tgtttaagag ggaagaattt
     6901 ataggagtaa tagcgcaata tatggaggaa aatcaagcta ttatcataac tactcatgag
     6961 atagtagaaa tagaaagttt agttgatgag gttataattc ttgatgaagg gcagattata
     7021 gcttcattta atgcagaaga attaagagaa agagaaggta aatctatatt agataagatg
     7081 agggaggtat ataagaatga ataaacttct atatctaata aatgtagatt tgagaagaag
     7141 caataagttt tatatagctt atatatcatt tttttcatta attacacttg gtttaaattt
     7201 attcgaaatt aataaattta aaaattctaa atttattata aacttagcaa taggagattt
     7261 tggaggtata ttttatggaa taggtatact tagtaatctt ggttttatcc aaagtatatt
     7321 gttctttggg atgttgggac tttttatata ctatattttt atgtggaaaa gagagttttt
     7381 ttcgaataat caaagtattt atacactaat gatgttacct caaaataagt ttaagatttg
     7441 tatatctaaa tcaatagctt tgttaacaat gatatatggt tttttgataa gtcagatagc
     7501 aacattattt atatgtaaat atatatttaa ttttattttt agaaatgtgc caataatcaa
     7561 tatgaacttt gccaaagatt tatattattt aaatttgaag ggtataccaa ttgattttat
     7621 ctcttttatg gctatttatg tatttttact acttatagct atttctattg taaattgttg
     7681 tatattattt atcttttcct atataaataa attttatata cttttaatca ctttgttgat
     7741 tatagtaggc ttatattttt caacaatata tataagtata atcactttat ttagaaaata
     7801 ttatatggct ataggatttc aatttaataa tttagtagca ggattattaa gttttttaat
     7861 tataatgttt attctaaatg gaatatctta tttgttaatt aaaaagaaaa tatctcttta
     7921 ggaggggtgg tatgaaatta aaatctaaaa tatctatact tattatattt ttgactttag
     7981 caatatattt tagtataggt ttattaaata atgaagataa cttaaagata aaaactataa
     8041 aaggagatcc taatgaatta gactatataa acataaattt tggatatgct tttaatggat
     8101 atgtaaattc atattataca ataaatggaa gtaacaaaac caaaaagact gaattaataa
     8161 aactacaaaa agatgctaag aacacaaata atttaattga tgtatctagt ggttcagatt
     8221 taattgctca tgttggattt gaaatgaatg atgactcaaa agatataaac tttgtaatag
     8281 atgaaaaaaa agaagattta gaaaaaggtg tatttccatc agaaggagat gataaagcag
     8341 ttgaatatga aaaactagat atgaatgatt ttacaaggac aagatataaa gtagataaaa
     8401 gttctgataa ttactatgca aatatactat catcaaaaag atataaaaaa tacttatatg
     8461 tatctgtact ttatgctaca agtgattcat caaaaaacaa caaagaatat tcaagtacta
     8521 tagatataat aaaaataaat ccagaaaata aaaaaatcca gactataaaa agtataaatt
     8581 taagtaatta tttttctaaa aaataccaaa atatagataa tataagtgta agttatgcta
     8641 gcaaatatga ggataagatg tatattttag taaatgtatt aaatgcaaat acaggtgata
     8701 aaagtaattc taaatttcac ttacttgagt ataatcttga agaaaataag gaaagcataa
     8761 tggaattaaa aactgatagc aaaatggctt tagcaaaagt aaagttagaa gataataaat
     8821 tatatatggt aatgtcaaat gactatgata atagttcaat tacagagtta aatttactta
     8881 cctataatat attagataaa agcttagatg aagataagta tattttagat aataaagaaa
     8941 aaagaggaat tagaagtatt gaaattgatt caaataaaat cattgtatat acatcaaggt
     9001 taaattctac attatcttgg gataataaat ttatgttata tataataaat aaaaattcta
     9061 aggaaattgt atatgaagga gaattacaat ttaacagttc aaatagtatg ttagaatttg
     9121 aaataaagta ggtgagttga tgaaagattt aataagatta ttcgaatttg aattaaaaag
     9181 aaatataaaa aattatatat ttataataat ctgttgttgt tcctttgtaa ttttaaacat
     9241 agtaaaaaat ttaaatgact ataattacat aatagaaaat gcagttaaaa gtgaacagtt
     9301 aacaaaaata ggaaatacta tagaaacagt aaatgctata ggtttctcaa gttttagaaa
     9361 tataatgggt tatacagaat catggtttat gtttggtata attgtatgta tatgctatgc
     9421 tttttttatt tggtatagag actttaatgg gagaagtaaa tctatatata cacttataat
     9481 gatacctaaa aatagaatta atatttatat atctaaatta ttaaatatat tatttctagt
     9541 gtatagctat acagtagtac ttacaatatc tttatttatt acaagtaagt tgttgcctag
     9601 acatatgctt gggaatgtta caagctatgg ttttgtacaa gaaacaatat ataatctaaa
     9661 aatgttactt ccatatagct ttgaaatact atttgtacag tatatatttt tattgatagg
     9721 atttgtatct gtagtattta catcaattct tataaataaa tctatttata gagtaagcgc
     9781 tttgataagt tttctatttt tagttttgga aattttagtg tttataattt ctatagaatt
     9841 tattatacct tacgaataca gtaatgtatt tactgttcta tattcaagta taaacatatt
     9901 tgtatgtagt cttatatcta ataaactttt gaagcaaaag attgactttt aggagggata
     9961 ttatgaaagc aaaaaatata ggaaaatata caatatgttt tataatagtg tgttttttaa
    10021 tgattactat gaccttaata ttgagttatt ttaataagga taaattatct gtaaaaactt
    10081 taaaaggcaa tgtaaaagat ataggtagag tgagtgtagt atatacacct aatataagta
    10141 aattttctta tgatgaggta attataagta aaaatggagt aagaaataat acaaatccca
    10201 catcaataat tagaaatgat tatttaaata ttgacaaaaa atacagtgat tttgttaatg
    10261 ataaaggaaa agatcttata tataaagatg atttgaatat aggagaagca tatacttctg
    10321 ttaagtacga tttagtacaa ggaatgaatg ctttaaatga ttgtataaag gtttcaaata
    10381 aaaatttaaa aagtggggca gaagaaaatt ttgatattaa tttaaacaag tctgatttta
    10441 aagaaaacag ttggaaaact ttgtttaata aaatatataa aggcaattta tatatggtga
    10501 ttagtgaaac taatatttca agttctaaag tcaataatat ttgtatatta aagatagata
    10561 taaaaaataa gtcttataaa ttattaaata gatttaattt agttttacag aatggatatc
    10621 aacaaaatat caatgaaatt aaattttcta ttggaagtaa attatattta gaaatagtaa
    10681 ataatcaaag tacaaatgaa gatattgaag aacatgataa ttactctaac tttattatat
    10741 atgatataga agaaaacact tttaatataa gtgataaatt tatggataat ctaaataaag
    10801 aaaagaatgc aaatccaaaa gagattggtt tattaggtaa aactaagcta gattatatgg
    10861 taaaaaataa taaattaaat cttatagtta cagatatgag taattctata acaaatcttg
    10921 tttatgatat taattctaat aagattaaat tagaagatta tgagatactt gatttaaaag
    10981 tggattataa tgaagcatca tctggaatta ggaaagttaa gttactggat gataagatat
    11041 attctatttc aggtataaag aataatctat caggagaatt aaaagggttt aaaggggttg
    11101 ttgatatagt tggaactaat ccggtaaaat ttaaagtatt tgatatgaaa cttaaaaaaa
    11161 ccgtttatga agctgagcta ataaatggag atgtaagttt acaagaaaag ttattttttg
    11221 ttgcaaatta agtgatggaa ttaattttat aactttaaac ttttatatgt attgtaggtg
    11281 aatataagta aaggagtgtc ttaaaatgaa ttttttagct catgagacac tcttttttat
    11341 atgaaatcaa atatattttt atcatttaga caatgaaaaa taagattatc tctattttaa
    11401 gacagacatt tgtttataat tatatacaaa aacatttttt aatatttttg tatagtatat
    11461 aataatttaa tttatattaa tacacttaaa tttgaaatag aatgagtata atggtaaaat
    11521 taattattag aatattagaa ataataaata aataatacat tgtacttaaa agggggagaa
    11581 atttaagatg aacaaaaaat atattaaagc aggaaattta attgatggaa caggaaatag
    11641 taccatgcaa aataagggta taattattga tggagatact atagttgaaa ttgaagatat
    11701 aagagaagat ttaaatggct atgatgtgta tgattactta gataaaactg ttatgccagg
    11761 tattatgaat tgtcatgttc acttgacaat ggagccagtt ggaaatccaa aagtatatta
    11821 tgataatgta tcagatgtag agcttgtagt aaatacgata aaacaacttg atgcttattt
    11881 ggattcagga gtaacatata ttagaagtct aggttgccca aaatatgtgg atgtacaact
    11941 taaaaatctt atagacaaag ggtctataga tggtcctggt atagttactt caggtccagt
    12001 tatatgcatg acaggagggc atggatacta ttttggtatt gaaagtgatg gagtggacga
    12061 atgtagaaaa tcagccagaa cagttttaaa aaatggagta gattgtgtaa agataatggc
    12121 aactggaggt gtaacaactg atggggttga accagggtca ccacaattaa ctttggagga
    12181 aatgaaggct gctgtagatg aagctataaa ggcaggcaaa ataactgcca cacatgcaca
    12241 aggaagaact ggtataaaaa atgcagtgct tgcaggaatt acttctatag aacatggtgt
    12301 ttatttagat gatgaaataa ttgatttaat gcttgaaaga ggaacttact tagttccaac
    12361 tgttgctgca ccatatttta ttctaaatgc aggaaaagaa agtggaataa ctgaagctac
    12421 tcttagaaag tgtgagattg ttgctattcc acacaaagag tcatttctaa aagcatataa
    12481 aaaaggaatt aaaattgcag ttggttctga tgcaggaact tcttataatg aacatggaaa
    12541 aacatattat gaaatgaaac taatgtcaga ttatggcatg gataatatgg atataatagt
    12601 tgctgctaca aaaacagcat ctgaactttt aagaattgat aaaaattatg gaactttaga
    12661 aaaaggaaag aaagcagatg ttatagttgt agatggaaat ccaatagaga atatagatgt
    12721 acttgcagat gtgttagcag tatttaaact tggtaaaaaa gttagataag taatataaaa
    12781 aataaaaaag agtgtctaaa gggattttta aataattata gacactcttt tttattagta
    12841 acaattaagt cttttatact ttagggacta gattctattt tatatttaga tataactctt
    12901 taaaatcatt ctatgcaata taatctatag aatctacaca tacaaagtat attcttgaat
    12961 cattagaatt tccaagtact agaacatcat ttttcttacc tagaacttca acaccagtta
    13021 gaaccaaact accagctact acagtaactt gattatttat attagacata taaagattat
    13081 ctaggatacc tttattacaa cagcagtcgt cacaatcaca gttacaatta cattctttat
    13141 aatgcttttt gtcaagatag taagctaaag atgatatgaa atcttcataa tctgcaggtg
    13201 taacattaaa ggcaacagct ttaagattac ataatgatgc taaatctaca tttgttaatg
    13261 ggttaactgt agaaattata taatcaatta tataatcaag tattgaattt agtatagaac
    13321 caaggtctat agtagcagga actgctgctt ctataagagc gactaattcg gatactcctg
    13381 ggatagtacc aattgaagta actagtcctc caagtgttaa aggaacaggt ttaggataat
    13441 acaattgacc agatatatca attaaatcac agttacaagc tgaaaatctt tcaaaagtgc
    13501 cattaagtcc agataaatta tcttttggag gtattgaata atctatacct actaaatatg
    13561 caccaacaat aaagaaatct gaaataaaag caaattgatt aaaatttaca aaaggtctta
    13621 gagaatcata tcttaaaagt tctaatgcct ctctcatgct ctttttacaa caacattttg
    13681 tcattttagg ttctttatat ttatttgggt aattatagta tctatcagct acttgataat
    13741 tttcatagta tctatcattt gcattcatat aatcttcttc gtattcttga gtaaatctaa
    13801 agtcctctct acatttatta ttttccatta aatacactcc ttttatattt tgatttaaat
    13861 gtatagataa ttaagtttat ctatatagta atattatatt tattgtgtaa aaaaatgtta
    13921 caaaagaaat tataaatgaa taaaaaataa taatttgtca aataaactca taaattatta
    13981 tgaataatct attctaaagt aaaaaactat gtataaaaaa ggtttatatg ttatatttat
    14041 ataaatataa catatgaatg agaaaggagt agtttgataa atgattgagg tagataaatt
    14101 atgcaagtct tttacaaggg ttgtaaaaga tgataagaat aaaagttcaa taagaaagtt
    14161 aaagaaaata aaaactaaaa aagaagaatt ttttgctgta aataatgttt catttaaagt
    14221 tatagaaggt gaaattgttg gaatattagg gccaaatggt gcaggaaaaa ccacattact
    14281 tagaatgtta ggtggcatat taacaccaac atcaggaagt ataaatgttt ctggatatga
    14341 ttattcaata gatagaaatt cagccaaaaa agaaataggc tatctgtctg gaaatacaaa
    14401 actatatgga aggctttctc ctagagaact tctaacaact tttgcaagtc tatatgagat
    14461 gtcaaaagaa gagatagaag aatcgattga aaatgttgta aaaataatgg atatgagtga
    14521 atttatagat aatagaattg aaaatctatc aactggtcag acccaaagaa catctatagc
    14581 tcgttgttta atacattctc caaaagtata catttttgat gagccaacac taggtctgga
    14641 tgttttaagt agtgcatcca ttatagattt tatgaagaat gaaaaactaa ggggaaaaac
    14701 tgttctttac tcaactcatt atatggaaga agcagaaact ttatgtgaca agatttttat
    14761 gattcacaat ggggaaataa ttgcatcagg aactccacag agtttaaaag aggaatctgg
    14821 agtaaataat ttgagagatg tatttataaa acttgccaga agggaggata tttaaatgag
    14881 aagtaagatt gtaaaataca tatttaaaaa agaaatgtta gacatattga gagataaaaa
    14941 gactttattt atgatgattg ttgtgccact tcttttatat cctttactta tgttgttgat
    15001 gattcaagta atgaatatgt ctgcaagtag tatgaatagc aaggacatta atatagcttt
    15061 caataataaa ccaaataaag tgttagtttc catgattgaa gatgataatt tagaaaaaaa
    15121 tattaaaaat acttcaagtg ataaaggaaa aataagagtg ttagatgtag ataactataa
    15181 aaagtcatta gaagaaaata aaattgatgc ttatataaag atagaagaaa aaaaatcatc
    15241 tacaagttat cgaatatata taaattcttc tcaagaaaat tcttctaatg cagtaaaaag
    15301 aattgaaact gttttggata aatataaaag atctactata gaaaagactt tatctgaaaa
    15361 aggattagat gtacaagcta ctttagaacc aataacatat agcactgtag atgttgcaaa
    15421 gacagaagaa gtggcaggat actttttagg tcagatacta ccatttatat taataatagg
    15481 agttttacta ggttcaatat atccagcaat agatgttatg gctggagaaa aggaaagagg
    15541 tacgttagaa acattattta cacttccaat atcaaactta gaactagtta tgggaaaata
    15601 tatggcagtt tctcttagtg caatagtaac agcaatttta aacattttat ctatgggact
    15661 gactatgaca tttattttag ttagtggagg tattagtaac caatttggct ttggaaactt
    15721 taattatgga gatttagtct tgccaattat aactacctta atttgcatat gtcttttttc
    15781 aatggtagtt tcagcaataa gtatgtgtgt atgttcatta gcaaaaagtt ttaaagatgc
    15841 ccaaaactac ataacacctc ttatgttaat tattatgata ccatcatatg tttctatgat
    15901 acccaatata gtattggata gagtaacagc tgtaatacct gtagtaaata tatctttact
    15961 tataaaaagt gtattatcct ttaaaagcga tttaaattta atagctttag ttttagcatc
    16021 aaatgtagcc tttgttatat tggcaataat actactgtct aagatgttca attctgagga
    16081 aatacttttt ggtaatagta agagtttctc tttcttagag aaaagaagta atataaaaaa
    16141 agggtctata ccaactgtaa gtgatggtgt tattttatat gcagtaggat tagttttatt
    16201 gatttatgtt ggttctttag ttcaaattaa atttggtata gtaggtatta taatgacaca
    16261 attcattata atttctttgc cattattatt tgcatactat attaaagctg attttaaaga
    16321 ggtatttagc ttgaaaattc ctaaattaaa gcacgtgttt gggagcattg ttctttggat
    16381 tggtggttat atacttatta tgattataac acagctaatt ttattcttat tccctcaaag
    16441 tatggaagtt gctgaaaatt taaataaggc tctctttatg aaagatagtg ttttattgaa
    16501 tttactagta atagcattat taccagctat atgtgaagaa atatttttta ggggatttat
    16561 attttcttca tttagtaaat caaaagataa aaataagtct atcaagctta ctataatatg
    16621 tagtagtgta ctgtttggta ttatgcatat ggattttatt agaataattc caacatcaat
    16681 attgggtatt atatttgcat acagtgtgta taaatctggt tcaatatttg tatcaatgtt
    16741 attgcatttt ttaaataatg gtgtagcagt tatgttaaat cattatacaa ctggaaatat
    16801 aatcaactta tataaatttg ttgaagttga tttttcgaat ttaaatgtac ttgaatttgc
    16861 gttaattctt ctaataagtc ttatattagt tatgttagga gcaagagctt taggtagaaa
    16921 gtctttaaga aattaagtat aaatttaata tacattaaaa tattaataag cactaaagta
    16981 tatggatgta tcctgtatat aaaaatactt tagtgctatt ttaattatat gaattattta
    17041 ttttagaaat ttatatttaa ataatcaagt ataaagtgag atattaaaaa atcaacttag
    17101 taattatttg atttattcac ttaaaaaaat taattattga tttgtcgaaa ctcttgcaaa
    17161 aataaaaaga taaagtatga atatgttgca aacactgtgt tttaatgaaa agtagtcatt
    17221 tcttgtaaaa tggatagttt atatttacct taattttgta aaattttctg ttactataat
    17281 tatagaatgt agtatattca ttgtcttaac tattgattta tatatttcat ataggtataa
    17341 tagaaaataa attggattat tttggcatat aattgtttat taaaatgtgc ttatttttat
    17401 ttaaattgtc tgaaaaattt tatttttata ttataatttt aatattttct tttcaaaact
    17461 atccatttta caagaaatgg aaacttttga gatgtacttg tttgccaata aaatagaggt
    17521 gttttaatag gaggaaattt ttatgaggaa ttttaaaaaa aaattgatgt tttttttatt
    17581 tatttgtata attttacaaa ttacatgcac aacatcagtc tattctttat gcaaaatcta
    17641 taaaaataac tccgtaaata actttgaatt tattgtgtct aataatcagc ttattagcaa
    17701 atatgcatta caaatgatac ttattatggc tacaatatgt attatactta tattctatat
    17761 aatatatgat aaattaaatt ttaaaattaa attgcaaaag atagcatata cagataatct
    17821 tacaggagca aatactatag ataaatttgt aattgatgct aacaaaatac tatgtaaaaa
    17881 tactcaagta aagtatgctt tattatatat agatattgat aaatttaaat atataaatga
    17941 cctatttgga tatgaagttg ggaatgaaat tttgcgtaat ttaacaaaga ttattaagag
    18001 taatatattt gaagaggaaa tgtttgcaag aatatctgcg gataatttta ttataattat
    18061 gaaatatata gaagaagaag atataacaaa caggttaaaa attatatttg aagaactaga
    18121 cttgtttaat aataatcagg aagaaaagta taagttagtt ttaagttgtg gaatctattt
    18181 tatacttcca gaagatagag atatcaactc aatcatagat agagctaata tacctcataa
    18241 aatggcaaag ggaggacata aaagttcata tgcattttat gataataaga ttcatgacca
    18301 agagataaaa gaaaaagaga tggaaaatac aatgttttca agtttagaaa ataaagaatt
    18361 tattatatac ttacaaccta agatagagct taatacagga gaaatacaag gttcagaagc
    18421 acttgttagg tggaaacgcc ctgataaagg attaatacct ccaaatgaat ttataccatt
    18481 ttttgaaaaa aacggttttg ttataaattt agatttatat gtgcttgaag aagtatgtat
    18541 ataccttaga aaatggattg atgcaggaat aaatccagtt accatttcag ttaatgtttc
    18601 aagaatacat ttatattgca ataattttat agaaacatac aaaaatataa ttgataaata
    18661 taacatacct gcaaaatata tagaacttga attaacagag agtatcatat ttgataattt
    18721 tgatatttta attgacataa tgaataattt aaaaaaaata ggatttttga tatctatgga
    18781 tgattttggt tcaggatact catcattaaa tatgttaaaa gaaattccta tggatatatt
    18841 aaaacttgac caaaaattta ttatggaaac ttataatagt aaaagaagca aaataattgt
    18901 tactaaggta gtagaaatgg caaaagagtt aggtatgaaa gtcatatcag aaggagttga
    18961 gacagaagaa cagtttgaac ttttaaagga agtaaaatgt gatatggcac aaggatattt
    19021 atttggaaag cctatgccaa ttgaagaatt tgaacattta attgtatcta atttgataag
    19081 agggtaatta atctgtatta taaaaattta gtaaattgtg ttaaaaatgg actctaatgt
    19141 agtgttcatt tttttttgta aaatttttta tgcttttatt aaagagtcat tgacatatac
    19201 tctgtattat tatattattg tattaacgaa ctaatacaat agtgtaaaaa ggaggtatga
    19261 atatggaatg ggaacttgat aataacaaac ctatatatat acaattagta gaacatttaa
    19321 agttaaaaat aatttcagga gaaattaaaa taggatctaa attagaaacc gtgagagcac
    19381 tagcagaaga tgctgaagtt aatccaaata caatgcaaaa ggctttaaca gaacttgaaa
    19441 gacaaggttt agtttatagt cagagaacca aaggaagatt tgtaacagat gataaggaaa
    19501 aaataaaagc tatgaaagag gaaatagcta atgtggaaat aaatacatta aaagtaactt
    19561 tagaaaagct tggatatgat agagatgaaa tactaaaact tattacagaa aatctgaaag
    19621 gagaattata aaatggataa aaatataaat aatatttctc aagatggaat agttgaattt
    19681 aaaggtgtaa acaagagcta tggaattaag actgttttaa agaatattga tttaaatata
    19741 ccaaagggaa aaatagttgg gctgttagga cctaatggaa gtggtaaaag tacaatgatt
    19801 aagttaatga atggattgtt acagcctgat aatggtgaaa ttatgataaa tggaatgaaa
    19861 ccatccatag agactaaaaa aatagtttca tatttaccag aaagaactta tttaaatgat
    19921 tggatgaagg tatctgattt gcttaaattt ttctatgact tttatagtga ttttgatgtt
    19981 agaagagcaa atgagatgat taaaagttta gatattgatg taaatgaaaa actaaagaca
    20041 atgtctaaag gaactaaaga aaaggttcaa cttatcttag taatgtcaag aaatgcaagt
    20101 atatatatat tggatgaacc tataggaggt gttgacccag ctgcaaggtc atatatactt
    20161 aagactatat taaaaaatta ttctgaagat agtactttgc tgattgctac ccacttaata
    20221 agtgaaatag aaaacatatg tgatgaggta atattcatat caaaaggaga aatagtgtta
    20281 caaggagacg ttgaagcaat aagagaagaa aaaggaaaat ctatagatgc attatttagg
    20341 gaggaattta aatgttagct aaattattaa aatatgagtt aaaagcaagt ggaagagtat
    20401 ttataccgtt atatatagcc atattgatag tagctgtatt taatggaata tttatgaata
    20461 caaatatact tcagatacag ggaatagcaa tactagtatt aacaagtctt tttatggcat
    20521 tagcagtttt aactattgta gttacaattc aaaggtttag aaaaaatctt ttgggagatg
    20581 aaggttattt aatgtttact cttcctgtta gcactagttc acttatattg tctaaatgta
    20641 taacagcact catatatgct gtagttagtt ttatagttgc aatattcact tttggagtac
    20701 ttatgctttt tggtacttct ggaattttat tacccgaaat tttagattta tttaacacta
    20761 gttttaaatg gatttctgaa aactttttag atattttagt attattattg gtaatgctta
    20821 cttcatattc attatttatt ttgttgttat atacatcaat ttcaatggga caattaccca
    20881 aatttaataa acatagaaat atagtagcat ttgcttcttt tatagctata aatatagtaa
    20941 tatcaattgt tggagatgca gttggaagca ttttaccaaa ggaaaataca aatatgatat
    21001 atcattttta tcaaagttct catgttatgt ttgcaatact tggtgatatt gtagttatag
    21061 tagcattatt tttcgctaca aaatttatat tagataaaaa attaaattta gaataaatgt
    21121 ttttatatgg agctatcttt tgagtaatgt tcaaagatgg ctcttattat ttatatattt
    21181 aattcatgca tataatatga ataaggtttt atttctagtt aaatatatcc atatgttgta
    21241 taatgatatt gtgtgtaaaa atttgtaaga ataactatac acttgatata agtaatatca
    21301 aaacaagtgt tttgtaagtt atattaaaaa aattaaaaat atatggattt aaggagaagg
    21361 tatgaaaagt tatacaaagg ctaagtggag tgtttggggt ataattacat tttcatttgt
    21421 tttagtatta tttttaagaa tgtcaactgc tgttgtttca gacaatttag aaaatgagtt
    21481 aggttttaat tctatacaaa tttcaaatat agcatccttc tgtctatatg catatgcatt
    21541 tatgcaaata cctgcaggaa ttttgattga taaatatgga gcaagaaaaa ttagcagtct
    21601 tggaataata atggcaagtt taggttcaat tttatttggg ttgattcaaa gtatagaatt
    21661 agcatatatt tctcgtgtaa ttgttggggc aggaacatca gtaatattac tttgtatttt
    21721 aaaagttcaa ggaagatggt ttaataaatc agaatttgcc tctgcaacag ccaaattttc
    21781 atttgtaggg aatttaggag gagttcttgc tacttttcca cttgtgttcc tgtctgaact
    21841 tgttggttgg agaaactcat ttttattaat tggtataatt ggtgttgtga ttggatgttt
    21901 tatgtatatt attgttagag atacaccaaa agagtatgga ttcaatgtag atactgaact
    21961 atatgaagag tcagagaagg ttaatattgt tgatggtatt aagtctgtta taaagaataa
    22021 atcaacatgg tataattcaa tgataatgtt atcttttgtg ggtcttacaa gtgcatttat
    22081 aagcttgtgg ggagtaagat acataatgga cgtttattgt gtgagtaaga gcttttctgc
    22141 gtttatagta tcatttttta catatggatt tatatttggt tcattaatta tggattttgt
    22201 atttgctaaa ataaggtcta gtaaatttaa tataataaaa tatggagcaa taatagattt
    22261 gttcatatgg attgtaattg tagttgtata tcaggtaaaa ccaccaatta tagttttacc
    22321 aatatcattc tttattatgg gatgtatagt tatgtcacat ctacaggttt ttaatgatgc
    22381 caagtataaa aataaagaaa tttattctgg attggcaact agtgttatta atacttttga
    22441 atttatagga agtggaatta ttaatttgat tatagctatc tctttacaag taaattcata
    22501 taatgtagtt gatggatata aaaaaagctt tgtagtattt atagtactaa gtattataac
    22561 tatagtatca tctcatatag gtgtgaaaaa tgatgatttt aaggcactat agtgttttac
    22621 taatagttta atttaatgtt atattttttt atatatataa caagtggttt atatttttct
    22681 aatatgtata attggaatat aaatcttgtt tactatgcac aaatggtatt gtattatatt
    22741 aaatagaaaa taagaacttt aaactataat attggttcta aaatataagg taaggagttt
    22801 tttatggata cgacaaaaat agatgatata agcaagttag atttaaagaa tctagatatt
    22861 aataagatgg atatgggagg tataatggaa agtctgttgg aatgggctac tacaagcgga
    22921 gtaaaactta taataggtat acttatactt tctattggat ttaaaataat aaaaaaattt
    22981 gtaaaccatg ttatgctatt acttagtaaa agagatattg atttaacttt aagaaggttt
    23041 ttgaagtctc ttcttttgag tgtacttaaa gttgctgtta taatcattgt tctagaatat
    23101 tggggaatga gtttgtccag ttttgctgct gtgatagcat cagccggggt agcaataggt
    23161 ttggctttac aaggtagttt atcaaatttt gctggaggat ttattatact tttgattaga
    23221 ccttttaaag taggagatta catagaagca gcgggtcatg gaggaaccgt tgaacaaata
    23281 ggactttttt atacacaatt ggttactcca gataataaac agatacttat acctaatggt
    23341 tctgtatcaa atgacagcct aataaactat tcagcaaaga atactagacg agttgacttg
    23401 acttttagtg taggatatga agatgatata ctacatgtta gaagagtact taaagatatt
    23461 gttaataacc ataaattaat aataaatgaa ccagaaccat ttataggagt agtagaacat
    23521 ggagataatg ccattaaatt tgcagctaga gtttggtgca atacagaaga ttattggaca
    23581 atttatcttg atttactaga agaggttaaa gttaggtttg acgaagaagg aattacgatt
    23641 ccatatccta aaatggattt aacagtaaaa gaattaaata aaatttaatt ctttgatata
    23701 gtgtttaaat ttaagaatat catatagata tttttataag atttgtaaaa aaataataag
    23761 tactgttatt ttgtaagtaa aaatataaaa aatatgagtg tctcaaaatg aactttttat
    23821 ttcatgaggc actctttttg tgtaaaatca aatacatttt tattatttca acaatgaaaa
    23881 agagacttgt ttatattttg agacagtatc ttttttgtgt agctttaatc ttagtaagta
    23941 taaagtttta aaatcaataa aatagtagaa atttttaata taaagggtaa ataaagggtt
    24001 tagagggcat aatatagaat aaattaatta tttaaggagg atttttatgt gtaaaaaaac
    24061 gtatttatta gtggtaagta tacttatagc aggtttatta actgcatgta gtgggagtcc
    24121 aactgcagat tcaggacaag cacaagataa tacaaaaaag gaagcaaatg cttcaaataa
    24181 tgctttaaaa gatgaaaaaa ataatgaaaa tttaatggaa caaaatttta aagtgccata
    24241 cacagatgca ataaacacat ttaaagataa atataaggat gctgatgtag tagatttaag
    24301 tcttgaaaga gatttaaata aatttgttta tactgtggaa ggtgtagatg ataataatga
    24361 atataagatg aaaattgatg ctaatacaaa agatgtattg gaagataaaa cagaaaattt
    24421 ggattcagaa gatttaaatg gagttgcaag aaaagaaaaa ttagatttaa atgacataat
    24481 tacacctcaa caagctatgg aaattgcatt aaaagagcaa aatggaatgg ttaaagaatg
    24541 gagtttagat aaagatttag atgttacttt ttataaaata agaatagata aagataaaaa
    24601 tgaatatgac ataaaaatag attctaaaaa gggaactgta ttaaaggtag aaaaagaaga
    24661 ttagaagatt taaattatct ttaaaataat tttttttgaa taaattttgt ttaaaatata
    24721 acaagcaggg tataaagaga ctaaataact attttaggag gagtacggta tgtcattaaa
    24781 atcactaaaa ataaaagaaa atttatattg ggtaggttcg cttgaccctg atttaagagt
    24841 atttgacata attatgtata caccttatgg aactacttac aattcatatg tattaaaagg
    24901 aacagaaaaa acagttttat ttgagacagt taaagataag cattttgaca attatattga
    24961 aagattaaat gatttaaata tagattttga aaaaatagac tatattgttg taagtcatac
    25021 tgaaccagac catgcaggaa gtgttgaaaa gcttttggat ttagctaaaa atgcaaaagt
    25081 agtagcttca gaaacagcaa ttaaatatct taaggaaatc gtaaacaaag attttgaata
    25141 tgttgcagtt acagatggag atacattatc aattggggat aaaactttag aatttttctc
    25201 tgtacctatg cttcattggc cagatactat atacacatat ataaaagagg acaaaactct
    25261 tgtaacatgt gattcatttg gtagtcacta tagtaatgat aagatagtaa atacacttga
    25321 tgaaaatgaa gaaaaggatt acctggatgc tttgagatac tattatgatt gtataatggg
    25381 gccatttaaa ccatctatgg taactgctat agaaaagata aaagatttag atatagacac
    25441 tgtatgtcca ggtcatggtc cagtattaac tgaaaatcct agaaaaatta tagaccttta
    25501 ttacaattgg agtgtaaatg aacaaataaa gttagaaaaa gaagttacaa tttgttatgt
    25561 ttctgcacat ggttatacga aaattatggc agaagctata aaagcatata ttgaaaaaaa
    25621 tagcgactat aaagtaaatt tatttgatgt aatagagcat aagcaagaag atattttagc
    25681 aaaaatatca gtatctcaag gtgtattatt tggaacacca acaatattag gagatgcatt
    25741 aaaacctata tgggatatat tagtatcgtt aaacccagtt cttcatggtg gaaaagttgc
    25801 ttcagtattt ggttcatatg gatggagtgg agaaggtata gaaaatgcta tggagagaat
    25861 aagtcaactt aggatgaaag cagtaaaacc ttttgccgtt aactttaaac catcaaatga
    25921 agagattgat aaattaaatt cttatacagg taagttccta gataagttaa acgctacttt
    25981 tggaagtaaa aagaaaacta agaaatttaa atgtgtaatt tgtaatgaag tttttgaagg
    26041 tgatagtgct cctagtgtat gccctgtatg tggagctaag gaagaccaat ttatagaaat
    26101 tgaagaagat gaagttactt ttagaaaaga tactgatgaa tacttcgtaa tagtaggaaa
    26161 tggagcagct ggtttttatg cagcagatgc aataagaaaa agaaataaaa cttgtaagat
    26221 aactatgatt tctaatgaag atgagttaac ttactataga cctgcacttt ctgatggtat
    26281 aaatgaagaa cttggctcag atttctatat ggaagataaa gcttggtatg ataaaaataa
    26341 tatagtagta atattaggaa cgaatgtaga taaattagat gaagtaaata aaactattat
    26401 agtgaatgat ggagctatta aatttgataa gcttgtaata gctacaggaa gtagaaactt
    26461 tataccacca attaaaggtc atgatttaga aaatgtgttt acacttagaa atataaaaga
    26521 tttatatagt gtaaaagaag cactagaaaa atctaagaaa gttgtagtga ttggtggagg
    26581 tttattagga cttgaagctg catgggaatt tagattaaaa ggattagaag tagtagtaat
    26641 tgaagcaatg gatagtatac tgtcaaagca acttgataaa gaaggtagta aaatattaga
    26701 acaatgtgta agagatacag gaatagatgt aagattaggt gtagcagttg atggtattga
    26761 aggtgatggc aaagctcaaa aagttatatt taaagatgga gatagtgtag attgtgatat
    26821 ggtagtattt tcaattggtg taagagcaaa cactcaaatg gctcatgata catctgtaaa
    26881 aattgacaga ggtattgttg tagataagac attacagaca actgttaaag atatatatgc
    26941 ctgtggagat gttgcacaag tggagaatac tagtttagca atatggccat cttcagtaga
    27001 aatgggaaaa atagcagggg caaatgcaag tggagataat ttgacatttg aaagtgaagt
    27061 atatccagtt tcattagatg caatgaatgt aaaagtattt agtattggta atatacaaaa
    27121 ttttgataaa gaaatttcat caaaagatga agggcaaaga atatataaaa aattatttat
    27181 gaaagacgat tcattggtcg gagctatact tataaatgat ttaagttgta ctgttaaatt
    27241 gataagatta atatctgaaa aaggtgattt tgaggatatt atgaaggctg atatactata
    27301 gtttaaatat actagaacaa aaagtcttaa aatatgacaa ttcatatttt aagacttttt
    27361 tgttataata tataataaaa taattacacc ttaactgtaa atttatatag attatttact
    27421 ttttattaat agattcattt tttgagatta gtatataatg aacatataga gtatataata
    27481 atagttaaag ttaaaaaagg ggaaaagcaa tatgcagtat atacaagaat tacttgattt
    27541 aactcaaaat tattgggtgt tagccacaat aataggtttg ttaagtgcat ttatagaaag
    27601 ctttatacca gccttaccac ttgttgctat agttacagca aatgctgcaa tacaaggatt
    27661 atttgttgga tgtttgttat catggattgg ctcaggactt gggacagctt cattattttt
    27721 attaataagc agatttaatg atagtaagct ttttaataaa ttgagaaata gtaaaactga
    27781 aaaagcaata agttggatgg ataaacaggg atttaaatta ttatttatag catatgcctg
    27841 tccatttatg cctggttgtt tagttactat cgcatcagct tttggtaaaa aagatgtaaa
    27901 agattttgtg ccagcaatgt tagcaggaaa atttgtaatg tttatagtta taagctatat
    27961 agcaagtgat atagaaggtt ttataacaag tcctttaaag atagcatcat ttatattatt
    28021 ggtgtttttg tcatggaaga ttggaaatag agtaaataaa aatcttgaaa atcataatta
    28081 tgatttccat cataaaaaac atcaaagtga taaagatgat aaaatgattt agcttgtacg
    28141 agataaaacg tatcttaaaa tgatttagta ctaaattatt ttaagatacg ttttatttgt
    28201 tgtattaaga tataaaattt tttagctctt ttgtgaagta aaatatacaa aacttatttg
    28261 tatttagttt gaaatagtgt tacttattgt ttctataact ataataaaat ataaaattga
    28321 tatccaatca aaaaaataac tatgaagttt aatagcttag atattaattt ttctgttttt
    28381 gagttagttt ttataggtag taaagaaatg tttatagtaa tgggataaaa caattcaata
    28441 ccttctttag taataaggtc aagacacaag tgagatagat atcctattat aaatccactg
    28501 gacaaacatg agaaaactat attattgtta gtgaagattg ttagagattt aaaaaaacta
    28561 taaataagta ataaggcaat tagactgtga gtaaatcctc tatgtctaaa cctgcttcca
    28621 aataatttgt atattaacgt aaatttttta cttatataag aacctctcat atctatatct
    28681 ggaaacaaag aacctaaata tgcaaaataa atataaacta taattaatac tattttatat
    28741 gcaatatcgt atttaactag gtacagattg tttataaagg ggagtatcaa aagtgctaat
    28801 atataacctc ccctttcatg tgtttctttt atcatataaa aatcctcctg aatcgtcact
    28861 ataaacatta tattggtaaa tttagactat cttttattat attatatagc aaacataagt
    28921 ttttttcaaa aggattttaa tatttaatta atcttgagat tttaaatttt tataattttc
    28981 taaagcacca ttccaattta ctgcattaaa ccagttgtca atgtattcat ttcttctatt
    29041 ttgatatttt aaatagtaag catgctccca aacgtcaagc cctattatag gagttaggtt
    29101 tttacttaca gggctatctt gatttggagt agtcataata gataatttcc catctttagt
    29161 agccacaagc caagcccaac cagaaccaaa gacatctaaa gcagcttttt ggaactcttg
    29221 cttaaatttt tcaaaagaac caaagtctct atcaatagct tcttttaaag attcagaagg
    29281 tatggttttt tctggtgtca ttatatcaaa aaagaattta tgattataag ctccacctgc
    29341 attgtttctt acagctgtgg caatatcttt aggtaaagaa tctaaatttt gcaataattc
    29401 acataaagaa taattataaa gctcaggata tttctcaaga gcaacattta atttatcaac
    29461 ataagcttga tagtgtttat catgatgcaa tttcattgtt tctttatcta tataaggttc
    29521 aagtgcatca tatgcataag gtaatggttt aactttaaat ttgttgtttt caggtgtaaa
    29581 agcaaatgaa gttatgcact gtgaaattat aaataaagac ataataactg gtattaatat
    29641 ttttttcttc ataaaaatca cctcctaata tagtatttct agaaataaaa aaaatatgaa
    29701 atattaacag acaatttata agaatttaat agcaatttca ttttaaattg aataagtttt
    29761 aaagccaaag ccaaaactat gaaatataga ataagactaa aatgcagaaa ggaattttaa
    29821 ttatatggac ggcaatattt caatttatta taaaggtgat atatctaaaa aagaaaatat
    29881 taaatttaaa ataaaagaat ctctaaataa tataaataat acaagagttt ctagtagaat
    29941 agagtataaa agtgaggcaa aaatttttga ttttgaaaga tttaatgtaa ttgatgttat
    30001 ttatggaaac aaaccatttg aaaaatatgg aaaagttatt gtttttaatg gaaatatata
    30061 taacaaccat gaaataaaag aagaattaat taaaagaggg cacagtttta ctacaggtga
    30121 tgatattgaa gtcttattgg catcttatat agaatttggt aagaattgtg tacataagat
    30181 taagggaatg ttcaacttta taatctatga tagagaaaac gaaagtatat ttggagcaag
    30241 agatttattt gggataaaac cactttatta tataaataag gaaagtgcaa taattttttc
    30301 ctcagaatat aagtttatac ttgaatatat gaaaaattta aatataaatg aaagaagctt
    30361 acaaagttat ttttcatttc agtatgtact tcctgaagat actatgatac aaggaataag
    30421 gcttatacca gcaggtcatt attttagggt agaaaatggc atattatctt taaagagata
    30481 taataaactt gaatttagaa gtagtacaaa atttttttat actaaaaatc acttaggtaa
    30541 tagagatgtg gataaggaag aagtaagaaa tattgtagta gattcaataa gaaatcatat
    30601 ggaagagcat aaagaaattg gaacatttct gtctggtgga atagattcat ctattataac
    30661 tactgtggct tcacaaatta atccaaatat aaaatctttt tctgcaggct ttagtgttaa
    30721 aggatatagt gaattggaag tagcaaagaa aacagctgat aaattaggta tagaaaatat
    30781 ccagattaat ataacgcaag atgaatatat aaaagcatta ccgaatgtaa tttattcttt
    30841 ggatgaccct atagcagacc cttcagaagt aggtctttat ttcttaatta aagaggcagg
    30901 taaacatgtt aaagtagcat tgtctggaga aggagcagat gaattatttg gaggatataa
    30961 tatatataaa gagtatagta ctatgaaatc agttgtcaac tcgccgactt atattaaagg
    31021 tatattagga agagtttcag aacttatgcc aaacataaaa ggaagaaact atctttatag
    31081 agctacaaca ccattagaaa aaagatatat aggaaatgca aaagtttttg aaaatagcga
    31141 agtaaaaaga ttctttttta agtataagga aaagaatata tatgaatacc tgttgtctaa
    31201 tttgtataga gatgcacaaa aaaataatta cgattatata agtaaaatgc aacatataga
    31261 tgtgaataca tggcttcaag gtgatatact tcaaaaaatt agcaaacttt ctacagctga
    31321 gcaggttgaa cttagagttc cttttttata taaagatgta tttgatgtag caaaaaattt
    31381 aagaatggag caaaaaataa acaagaataa tacaaaagtt ttacttagag aggcatttag
    31441 agaaatagtc ccagaacatg ttgttcaaag aaaaaaatta ggatttccaa ctccaataag
    31501 agtttggtta aaagattctc tgggggggat tgtaaaggaa actatctcta attcaaatgt
    31561 agacgaattt atagataaaa aatatgtaat aaaactttta gatatgcatc ttaaagggca
    31621 tagagataat tcgagaaaaa tatggactat atttacattt tgtctatggc atcaattgtt
    31681 tattgagcat aaaaatgtcg agtattaatt atgttttcat attaatcaga gggcaaaaac
    31741 atgtactgtt ttttccctct tgattattat ggaatagtac aaatagaact ttgattgatt
    31801 agtttactat ttcaataaaa ttattggcaa atattttgac aagactactt tctacagcta
    31861 aatttaagtt ttgtccatca tcatatccag cagaaatcaa tcctagtaag tttccaaaca
    31921 tatctaaaag tgctcctcct gaactaccac tagagattgg agctgtaaat tgtatcattt
    31981 gaaccgtttc aaaatcacga aaagctgaga taattccatc tgaaactgta ttaaaaagac
    32041 ctaaagggct tccaattgca actatttttt gacctcttac aggtttttta ccgattttaa
    32101 caggtattgg cttacatttt ctatcaactt ttatcacagc taaatcataa cctgaatggt
    32161 attttatgat ttggtaagat gtgtaaactt tatcatcatt ttcaaatcta actaaaaatg
    32221 aataaccatc attgactaca tgaagattcg taagtatata accttcatta tttattacta
    32281 ccccagaacc gcttttaaac ggtttatttt ctttattgca cacaacaatc ataacaacag
    32341 aagctgataa ctttgcaagt tcttcatagg aaagttctac ttcctcacct tgtacgattt
    32401 ttttagatat taaatttgtg ttattaaacg tattttcaga gttaggttta ttcatagggt
    32461 gggaagtctt attggtattt tcattgtaat ttatatttgc aatatgcctt tcaaaaggta
    32521 tttctaatat atttggagtt ttcctagaaa gttcaaatat attagaaaac tttgaacatt
    32581 caggatataa atcaagaata tctttatttg gatttatata taaaatatca cacccaagtt
    32641 gtgataaaaa atataagaaa aaaagttctt gtcttttaat atttccaaaa tacaatatct
    32701 ttggtgaaat attagtattc accttgttat aaaataattt aggtagaaaa tattctatcc
    32761 aacacaaaag tttaactgca aagtttttga tgatagaggg tgtacaattt ggcatactca
    32821 tggaaaataa atctaaagta tttttaaatg caatcttttt agtccaattt aaagtatcat
    32881 tttctatttt acatgggaag ttgatattat ataaatccca actgttaaat ttagcactat
    32941 cataaatatt aaaaatttta agcatcttat ctgtttgttc tttgtcaaaa tttatgttta
    33001 gactgcttaa tctaatatag ggaattatag gattattaaa aaaataagta tctatatttt
    33061 taattttttc tatgtattta tcaattgaat catcaacacc tatatatctt gtaaagtaga
    33121 cttctttaat atatgaagaa ctcttatttt gataggtagt attagacata aattcaatta
    33181 gaggttcatt tgattttttt attgaaatct ttagtggttt aatagtacaa atcatatttt
    33241 ttctcctata ataagatgat ataatctatt ataaaacaac ttaaaacaat atagtacaat
    33301 aattttgtag tttcaaaata aaatatgtac ttgtttatat aaatgatata taattacata
    33361 ttaaacatag cttttataca tttaataaag tatgtaaaat ctatgtaaaa agtaagaaat
    33421 ataaacattt ttttaattat attttaaaaa tatcaagcaa gttttcttca caaatgaata
    33481 aaaaatgtta taatatgatt aaagaaaaag ataaataatt agcaagattt gtttagctaa
    33541 aattaacaag ggagcgataa attatgggta taacattatc aaaaggccaa aaggttgatt
    33601 taactaaagg aaatccaggt cttaaaaata ttttagtagg tttaggatgg gatactaaca
    33661 agtatgatgg tggatttgac tttgatttag atacagcagc atttttaaca ggagcaagtg
    33721 gaagtgttac taatgatgga gattttgtat tttacaacaa tttaaaacat acatctggag
    33781 cagttgagca tttaggagat aacagaactg gcgaaggtga tggagatgat gaacaaatag
    33841 ttgttgattt atctaagatt cctggagaaa taagtaagat ttcttttact gttacaattc
    33901 atgatgctac agaaagaaga caaaattttg gtcaagtaag taattcttat ataagaatag
    33961 tagataaaga cacaaatgaa gagttaataa agtatgaatt aggagaagat tttagtatag
    34021 aaacagctat agtagtagct gaaatatata agcacaatgg agaatggaag tttaatgcac
    34081 taggttctgg ttttgaaggt ggtcttgctg cattatgtgg aaactttgga ataaacttat
    34141 aatttcaact ataataataa actaaagggg gatattttcc atggctatag tattaaaaaa
    34201 aggacaaaaa atcgatttga ctaagggaaa tccagggctt aaaaatataa aacttggact
    34261 aggatgggat acaaattcat ttgatagtgg ctatgattat gatttagatg taagtgtttt
    34321 catggttgga gaatctcaaa gagttgaaaa agatgaagat tttatattct acaataactt
    34381 aaaacatcca tctggagcag ttgaacactt aggtgataat agaactggtg aaggtgatgg
    34441 agatgatgag gaaatattgg tgaatttaga ggcaatgcca aagcatatac aaaagatagc
    34501 tgtaacagtt acaatatatg aggctgctga aagaagacaa aattttggac aggtaagcaa
    34561 ctcatatata agagtattaa attctgaaaa tgatgaagaa atattaagat atgacttagg
    34621 tgaagaattt agtatagaaa ctgcaattac ggtatgtgag atatataagt ataatggaga
    34681 atggaagttt agtgcagttg gagcaggatt tgaaggtggc ttagaagctc tttgtagaaa
    34741 ctatggctta aatgtttaat ttaagtaata taatatatta aatataaata acctatacaa
    34801 ataatgtata ggttatatta tataaaattt tgtggaggtt tagtatgtcg attaatttat
    34861 caaaaggtga taaaatagat ttaaaaaaat ctaatccggg actgagtaac atacttgtag
    34921 gtctaggatg ggaccctgtg caacaatctg gtggaggctt ttttaaatcg ttgtttggtg
    34981 gaggacaagc tgatatagat tgtgatgcat ctgtatttat gcttaatcaa gaaggtaaat
    35041 taagtggaat aaaagattta atttattttg gaaatctaaa aagtgcctgt aagtcagttt
    35101 tacatacagg agacaatcta actggtgagg gtgcaggtga tgatgaacaa attctagtta
    35161 atttggataa agttccatca aatattcata aattattatt tgtagtaaat atatacaatt
    35221 gtgtggatag aaaacaacac tttggaatga tagaaaatgc ctatataaga gttgaagacc
    35281 aaggtaataa gaaagagata gcaaaatata atctatctga taattattct gaaaaaacta
    35341 ctcttatagt aggagctata tatagaaaag atggcagttg gcaatttaaa gcaattggtg
    35401 aaggaacaaa agatgcgggt cttaaagaag taatgcaaaa tttagacagg attgagtgtg
    35461 catatggaat ttaataatga tatcaaatat tataatgtag ataaagaaga agttacaaaa
    35521 gacttatctg ttaatcctga aaaaggttta tcagaatcag aagttaagac tagaagagaa
    35581 aagtatggtt tgaatgaatt tactccaaaa gaagagggta gtttttggga tgacttaaag
    35641 gaaagtttga gtgaaccaat gatagttatc ttgattattg cagcagtagt aagtgcagta
    35701 attggggaaa ctcatgatgc aataggtatt gtaggtgcta tagcaatagg tatagcaata
    35761 ggtatgatta cagaaggtcg ttctaagaaa gcagcagaag cgctttctaa gttgactgaa
    35821 aacatagaag taaaagtatt gagagatggg gaagtacatc aaatttctaa gagtgaattg
    35881 gtaccaggtg atatagtata tattgaaact ggtgacatgg tacctgctga tggtagactt
    35941 atagaaagta taaaccttaa aattagagaa gatatgttaa caggagaatc tgatgatgta
    36001 tctaaaaagt gtgatgtagt agtttctatg gaagagatag aatctaaagg agcagtagta
    36061 gtacaagagc caatacctgc aaaacagata aatatggtgt ttggtggtac acttgtagct
    36121 tatggtagag gagctctagt tgtaacttca attggagata atagtgaaat gggtaaaatt
    36181 gctcaaaacc tatcagaaac agatgagcaa actccacttc agataaaact tggaaattta
    36241 ggtggcttga tagccaaggt ttctagtgca gtagcaggat tattgtttat atttatggtt
    36301 ttccaaatgg taagtaagaa tgtattaaat gtagatatgt ctggaatatt accattttta
    36361 gaatcaatag acccagttaa aacagcgttt acagtatgtg ttgcgttgat agttgcagca
    36421 gtaccagagg gtcttccaac tatgataaat atgacacttg caataacaat gcaaaaaatg
    36481 gctaaaataa atgcattggt tactaaaaaa gaagcttgtg aaacgatagg ttctgtttct
    36541 gtgatatgtt cagataaaac tgggacatta actgaaaata gaatgactgt agaagttgca
    36601 tatgttgatg gaagatatat tgaaagttca gaagaggaat taaatagtta ttttgaagaa
    36661 aactgtatga taaactctac agctgatgta gagcacaatg atggtgatat taagtattta
    36721 ggaagtgcta ctgaatgtgc attgctttta tattacaaaa acgtagatta tagacaagct
    36781 agaaaaaatt ctaatatagt agctcaaaat ccttttactt cagattcaaa gagaatgact
    36841 tctgttatag gtcaagataa tcatcatgta ttattatcaa aaggagcacc agaagtctta
    36901 cttgagttat gttcacatat tcaaagagga aaagacatag ttccattaac tgaaaatata
    36961 aaacatgaga ttttagaaga aataaagaaa ttgcaaataa aatcaatgag aactttaggg
    37021 tttgcatata aagaaatctc acaagctgaa gaagaagcag cagtaactgc cgaaagtgat
    37081 gctatgaatg taagtgcaat ggaaaataat ttagtattta gtggttttgt tggcataaga
    37141 gacccactta gaaaagatgt tatagagtct gtaaatacag caaataaagc tggtgtttct
    37201 gtaaaaatgc ttacagggga taatataaat acagcaagag ctataggtga agagttaggt
    37261 ctattaaaaa ataatatgag ggtggttgaa gcttcatata tagatacgtt agatgatgaa
    37321 gagttaaaag aagagataca aagtatttca atcgtggcaa gaagtaaacc agatagtaaa
    37381 atgagaatag tttcagctct acaaaagagc ggagatgttg ttgcagtaac gggtgatggt
    37441 ataaatgatg ctccagcact ttcaaaagca gatgtcggta tagcaatggg tatatctggt
    37501 acagaggtat ctaaaaacgc agcggacata attttaactg atgatagttt tagtaccatt
    37561 gtaaaaggta ttaaatgggg tagaggaatt tatgataact tccaaagatt cgttcaattc
    37621 caattgactg taaatgtaat tgcattttta gtggctataa tatctcaagt tatgggacaa
    37681 gagatgccat ttactacaat acaattatta tgggtaaaca taatcatgga tggaccacca
    37741 gcactagcat taggtctaga accagttaga gaccatgtat taaatagaaa acctgttgat
    37801 agaaaagcca atataatttc taagtcaatg gtgtatacaa tagtattaaa tgcattctat
    37861 ataacagcaa tactaatgct acaatctaca tttaatttct taggagttga cgaagctcat
    37921 aagggaacag taatgtttgc attatttgcc tttagtgcac tgtttaatgc gtttaactgt
    37981 agagaattta atgcagatag tattttccct aattttacac ataacaaatt agcactgcaa
    38041 ataatagtat tgacaggaat tgcacaagta atatttactc agttattcca agatttcttt
    38101 aactctgtag caatggattt tatgacatgg gtaaaagttc tatgtgtagc agcaagtgta
    38161 attgtggtta atgaagttgt aaaatgtata ataagactgg taaaaccaaa aaagactgct
    38221 taaaacaaat tttttagaag agaacagact gactcaaaat atgaattttg ggtcagttct
    38281 gaataaacat ttaaattggg aggatagtta tgggtgactc aaaggtattt gaaaaaatat
    38341 tctccagcca atcagataga ggaaattata ctccttcaaa gggatatcta agttatttta
    38401 tttcatatat tggtttagaa gatgaagttt tatacaatct cgagatattt aagacaaaac
    38461 aaaatattga tagcaaaaaa gatattgctt tatttactga tgttatagca aatcctagtg
    38521 attttgatat tataaattat tttaaaaatg gacttcaaaa atatagaact agtatggaag
    38581 atgtagatat aaatatttta ggatttgaag agattgatta taaaataaag caagcaatgg
    38641 ataaagtgtt aaaagaggaa gaaaaagaat ttactaatga tagagttaag caaaatttta
    38701 tagtgaaaat aatggcatgg ataaaaattt atatcggtgc tttagatata aataaaaatg
    38761 aagctccaaa agtaatattt tatggagata taaaaaaaca tgaagtttat ttactcttaa
    38821 tattgtactt ggcaggtttt gatgttttat atcttaatcc aaattcaaag agtaatatag
    38881 atattttaaa atcagaaaga tacaatgtag aatttgaaga agccaacata atagaagaaa
    38941 aaacttcttt tgaagaaagg gttattttag gtgaaaaaat agataaaagt tctgtaaaaa
    39001 aagcttttac tgttggggca gaagcatcaa aacgtataag tgaggaattg ttaaatgatg
    39061 caggatttat aaaaccttgg caacttcaag atagaaaaat aaaaaatcta cttctaagtt
    39121 caaccgttga tgaaatatca atatactgga atcagccact taaattaaga ccaggattta
    39181 attttaatga tgctattgta gaagctccaa attttctttc taagattaat ggaatatata
    39241 atgataagaa tgaatatatt aagtttttag atttattaag agattcggaa agttcaacgt
    39301 ttattgaatt taatggagat gtagatagat tttcaaaagc ttttacaaga gaagctttta
    39361 gtttatcctt tttattggat tcaaaaggtg taatagataa aaactctgtt ttaaataata
    39421 aagattattc catatcaaca ctagcattaa atcaacaaat tatgatttta gaaaaagtgg
    39481 aagagttatt agaaggaagt atgtttttga atggtttaag tggagaagac aagataaaag
    39541 gtctatttac agtacttcac atggataaga agtttgttca catgatgaac aattttgatt
    39601 actcacttat aaatccaaaa ttgattatat atatgtataa gagtattata tttgataaag
    39661 aaatagtttt tcttatgctt ttacttagta agattggatt tgatattatt atcctgtgtc
    39721 caggaggaga aaataatata gagaatgtta taaataacca attgattgat gtacacagat
    39781 tggataaaat ggtatatgac cttaaattaa attcgcttga aaatgatata cctttactta
    39841 aaaaaatttt tggaaagagg agacgatttt agatggggat aaatataaat agtttttcaa
    39901 aagataagaa ctctgtagat gttactgaga atttgcccac agcagttcaa gaagaaaatt
    39961 tcgatataat ggaatataca aataataaaa aaaatgaatt gagaaagtct aaggaagttg
    40021 aggcattgac ttctcttata gaagtagaaa atccagacac gattttacag tttggtagaa
    40081 aagcatctga aggagtagca agagtttctg atagcctttt aaatacaata aaattaaata
    40141 gaaatgaaga aaattctaaa atgttggttc atttaactaa aataatggac aagtttgatt
    40201 tagatgattt tcaagagact aaggaaccaa atttcgttca aaaacttttc aaaaaagcta
    40261 ataacgccat tgaaatgatg tttcaaaagt atgaaacatt aggaggagaa gttgaaaaaa
    40321 ttcaaattga gcttgagcaa tacgaaagag atatagcgct ttctaataag caaataggtg
    40381 ctatgttaaa tgaaaacttt gagttttata atgaacttca aaaatatatt gttgctggag
    40441 aaatggcaat agaagaaatg gataatgaga tactaccttt ctttaaacaa aaatcagaga
    40501 ctagtggaga tcaaatggat gttgttaatt atcaagaact attaaaagtt tatgatatgt
    40561 taaatcaaag agtctatgat ttaagaatag cagaaaatat agctattcaa acaataccta
    40621 tgcttagagg tatgcaacat aataattatg gtttaattag aaaaataaat agtgcatttg
    40681 ttgtaacttt accagtattt aaacaatgtt tatctcaagc tatattactt aaaaagcaag
    40741 aattacaagc aaaatctcta aaagcacttg atgacaaaac taatgaatta ttgcttagaa
    40801 atgcacaaaa tgtaagttct caaagtgcac aaatagctag aatggctgga acaagttctg
    40861 ttcaaataga aacattggaa aagatgtaca atactatcaa gtctggtata gatgaaacta
    40921 tgagaataga agaaaacaat agagctttaa ttaaagataa tacaaagaga ttggaagaat
    40981 taaatacaac tataatttat aataagtagt attatattaa gggctaattt atataaagtt
    41041 ggccctttct atatatgagg aggctagaca ttgaaatatt tcgattatat ctgtaaagat
    41101 gatttggaga gaattttttt gaaagagcca gaagacttta gtgctaagac tgaaaaagat
    41161 gtactgaaat atgcactggg agctttttta tacgttccag caactcaata taatatgatt
    41221 tataagagta ttatagggga tgttaaggga gttagaccat tagctatttg tttagaagat
    41281 gctgttggag taaatgggga gttagaggct atagaaaacc ttagattaat tttaaaaaat
    41341 attagcaatg aaagtataac taataaagat ggaataccac taatttttgt aagaataaaa
    41401 gatgtggagc aactgttgag aattaaagag attataatta aaaatagtca ttctataacc
    41461 ggaatattaa ttcctaaagc aaactcggaa ttaattgaaa attgtataga agctttagat
    41521 tctatgaatc ttcaagatat gtatgttatt ccaataatag agacaagaga atttatatat
    41581 aatgaaaaaa aagaacttag ttttattaat ttgtacaatg ctattttaag acataagtca
    41641 agaatattaa gcattagagt aggtataact gatatattag ggatgtatgg tataaggaga
    41701 gataaaaact tttctatata taataatttg atttgttctt catttatact agatataatc
    41761 acatatcttc aaagaccaga gctagatata cctataagtg gtggagtgtc agaatttttt
    41821 gatatgaaaa ataaggacat aagaaataag tatatagaag aaatattatt agataaatat
    41881 catggattaa ttggaaagac tgtaattcat ccaatgcaaa ttcaaattgt acaagcgtta
    41941 tctacagtat catatgaaga ttttactgat gccttggata tattggatag tacagacagt
    42001 aaatatggtg tgagtaaggg tgtgttagga gagagaatga atgaaataaa tccacatttt
    42061 ttatgggcta aaaaaacatt gattctatct aaaatatatg gtgtattaaa tgaaggagta
    42121 gactatgaag aattgcttaa attttaattg tgagattgac attattgata atccattaaa
    42181 tttagaacta aatgattttt tagacatagg aataagaaat aatagcaaaa gacgtttttt
    42241 gtttatctca aagaaacttg gtaagcattt agcatgtaag ccaagtgaga tggataattt
    42301 gggaaaatta atagctacaa tttataatga aaaaaataag gaggaatcaa atggtactgt
    42361 tatagctttt gctgaaactg gtacagctgt tgggcatagt ttttttgatt atttatgtgg
    42421 agattatgaa tttattcata ctactagaga acagtttgac gagttagaaa atctggaatt
    42481 tttagaagaa cattctcatg caacagacca aaatttatac tttggtatgc ttaacaactt
    42541 taaatttgga gatgagataa tattggttga tgatgagatt accacagcca atacatgcat
    42601 gaacataatt aggaagattc aaagcatcta tcctaaaaag aaatacacta tatgttcaat
    42661 actcaattgg gtaagtgatg aaaatttaag taaagttact cgtttagaaa gagagctaga
    42721 ttgtaagata gattttgtat atttatttaa aggaaacttt aaattttata tagatgaaga
    42781 tattatttca aattataacg ataattatac tattactaaa ttagatacta ttttaagtga
    42841 taaagtaaat ttagtagtaa atcatattta tgttgattta gaagattaca ttggaaataa
    42901 gaaatatgta aaatatacag gaagatttgg aattaataga agagaacaat ttagattaaa
    42961 agaaatcgtt aagagagaaa gtaaaaaaat aactatagac aataaagaat ctttaaaaaa
    43021 tgaaaaagaa aagattctat ttttaggtac tgaagaattt atgtatatac ctatgctgtt
    43081 tgcaaaagaa aaagaaaatc aatgtgatgt atactatcat tcaacaacaa gaagtccaat
    43141 tattaatatt gataagcata attatcctat tagaagtaaa tttatgctaa agagttttta
    43201 taatatggat attcaaaatt atatttataa tatagataaa cagaattttt ctaaatgtta
    43261 tttatttgta gagctaaata aaagtgaaaa ttcttatcta gagtttattg atataattaa
    43321 aaagacaagt ataaaagaat taactatagt atgttgtagc tagagtgttt taacaggata
    43381 aaatatatca cgattttaag ataggagttg agtcatatga gagattttag tacgtattct
    43441 aaagatgatg ttattttttt attaaaagat atatcaaatt taattgaaga agaaggaaat
    43501 aaagaaagag aattaaaaat acaaaatggt agacattatt cagaaatgat accaatagag
    43561 tatgaagtta gtgaagatta tctgaattta tatcatgaga aactaagaga aaataaagat
    43621 aagcttgcat tttctatagg cgttatgtgt gaaaaaataa ttaaaaaaaa tggaaatgat
    43681 gtagtactcg tttcacttgc tagagcagga acacctattg gtattttggc aaaaagatat
    43741 gtaaaaagca agtataatat aaatttacct cactatacaa tctctataat aagagataag
    43801 ggaatagata tgaatgcaat tgaatatata gtaaagaatc atccgagctc aaagattcaa
    43861 tttttagatg gatggactgg taaaggaact atatcaaaag aacttgaaaa agcatgtgat
    43921 gagcttaatg ataaatttaa aaaacatttt gactcaacac ttgctgtatt agctgaccct
    43981 gctggatatt caggacttta tggaattaga gaagatttct taataccaag tgcatgttta
    44041 aattcaaccg tttctggtct agtaagcaga actgtactaa gagaggattt aattggtaaa
    44101 aatgacttcc atggagctaa gttttataga catttaaaag ataaagatga gtctatgaac
    44161 tatattgaaa ctatagaaga atgttttaaa aatcaattta agaacatatc tgatgaagtt
    44221 gaaaactggg aaagtgatat tataacaaga gatggatact ttgatgtttt aaatataaaa
    44281 gaaaagtata acataacaga tattaacttt atcaaaccag gtgtaggaga aacgacaagg
    44341 gttttactta gaagagttcc gtataagatt cttgttaaag acttaaatga taaatcttta
    44401 gaccatatat tcgtattagc taaggagaaa aatgttgaag ttgaacaaat ggatttaaaa
    44461 gcctataaat gttgtggaat aataaaaaat atgaaagatg tttaggaaat atattagaca
    44521 ggagaatatt gaatgattgt tttttcagat ttagacagga gtatcattta ctccaataaa
    44581 tttttaagtg ctgatagtaa atatgctaat atagaaattt atagggaaaa agaaatatct
    44641 tatatatctt tagatactat aaatttaata aagcaaatac aatattatgg tatgtttata
    44701 cctacaacta ctagaacagt ggagcaattt aagcgcatag agtttaataa atatgggatt
    44761 tattttcctt ggagtataac ttcaaatgga ggagttatac ttaaagataa tgaaatattg
    44821 aaatcatgga gtgaaaaaat agataaacta aaatctgact atgagcccat tgaaagcatg
    44881 atttataaat ttaaagacta tttgaatgta gatggaataa ctaattttaa agttgcagaa
    44941 gatacatttt tttatatagt tgttgatttc tcaaaattta atttagaatc aattaaagaa
    45001 tatacaaatg tattggaatc aaaaaattgg aagttttatg tgagtggcag aaaaatatat
    45061 tttataccaa aagaaatttc aaaagaaaat gctataaaat acttgaccaa agaattagga
    45121 atagaatatt tttatgcaat tggcgactct attatggact atggtatgct taatatctca
    45181 aataaagctt atctattgaa gcatggtgat ataaataaaa atgaaataga aaattcattt
    45241 ttatcaagtt cttttaatgg aatgagtgga actgaagaga tactatccaa cattttagat
    45301 gaaaactgtc tacttaatat ataatgactg atatataaat aataagctag aaaaatctga
    45361 aataaattag aataaagttt atcatactaa tttatttcag atttttatca atttatattt
    45421 aagtaagaac tcaatatgtt aaaatgaagg tggtgtcatt gcccagactg agatacatac
    45481 atcatcatca taattttcat atacatgtgg gatagtactg ttaaaataaa aactatctcc
    45541 ttcttccaat agatattcct tatttccaag tgttatcttc attttacctt ttattatata
    45601 gccacattct tctccttcat gtgttacaaa ttcactgttt ttttgtgtat gaccatctaa
    45661 agtaacaagt ataaattcta tttttttccc agataaatta ggaacaagaa gttcgtaaaa
    45721 ttttgtagaa tcattagtta ctattttttt tcgattattt ttctttacta ctatatcaga
    45781 gtcctctctt ccaatctctt caaaaaaata acctatattt atatcgaggg cttttgatat
    45841 tttccacatt gctacaatag atggaacaac catattacgc tcaatttgac ttattaagcc
    45901 tgtacttaaa tctgaatttt ttgctaaagt agagatactt atatttttag attttctaag
    45961 agatctaatt ttgcttccga catctatatt cattagatac aaacctcctc agacatgatt
    46021 ataacatata atttataaag attaataaaa taaaaaaagt tttataatac atgtattttt
    46081 tctgttatat tgaattttct gaaaagatgc attataatag tattaatatt caatatattg
    46141 aatattgata tcgatatttt aatttaatga aatggaggta gttaagatga agagttttgt
    46201 aagtcctgat aatatttggg tattatgggc agttgtaact ggatgggcag catttagtat
    46261 ttatttggaa caaaagtaca attgggcatc aaaagtgtct ggagcaataa tagctcttgt
    46321 aggagcaatg ttattatcaa atctaaatat tataccagta gagtctgttg tatatgacca
    46381 agtatgggga tatgttgttc ctttagccat agctttattg ttatatcaat gtaacattaa
    46441 aaagatatgg aaagaaagtg gaagattgct cattatattt cttgtaggtt ctgtgggaac
    46501 tgtacttgga gctatgattg gatttttagc attaaaaaat gtagtaccag atttaaatat
    46561 tgttgcagct atgatgacag gttcttatat aggtggaaat gtaaactttg ccgctatgtc
    46621 aggggcattt gatgcgccag gagaattagt atcagcaaca gttgtagcag ataatctttt
    46681 aatggcatta tatttctttg tcttaatagc tataccatca attggatttt ttagaaaaca
    46741 ttttaaacat ccacatgtag atgaaatgga aagtataggt gtaactgaag gagaaacagt
    46801 agcagcaaat tattggggaa gaaaagaaat atctctaaaa gatatagcaa tagccattgg
    46861 tagtgccttt ataattgttg ctgtatctgt agaactatca acttggttca aagctataat
    46921 accattatca aatccttttt tagcaatgtt aaatacttta tttggaaatc aatatctaat
    46981 aataactact ttgactatgt tgtttgctac atttatgcct aatttctttg gtgacataaa
    47041 aggagcacag gagttgggta catttttaat atatatattc tttgttgtag taggcgttcc
    47101 tgcttcaata acattaataa ttcaaaaatc accattacta cttttatact gtggaataat
    47161 ggtactaata aacatgctag ttacatttat agttgcaaaa atatttaagt ttagtttaga
    47221 agaagctatt cttgcatcta atgcaaatat tggtggacct acaacagccg cagccatggc
    47281 gatatcaaaa ggatggtcta agctagtagg gccaatactt atagttggta catttggata
    47341 catagttggt aattattttg gtcttttagt aggaaacatt ttgatataat tttacgtgaa
    47401 aaaaagttgt tttttagaaa gatttttatt taattggagg ttgttatgat tttcgatgga
    47461 cattcagata cttgggcaga tgtaacaata agaaggctta atggggaaaa agatgtattt
    47521 agaagaatcc atcttgataa ttttaaaaga gcaaatatag aaggagctat ttttgtaata
    47581 tggatagacc cgccatatga taaagagcct gaaaaaagag ttaaacaaat cataagctct
    47641 ataaaagatg aaattgaaga tatgaaagat gttgtaaata tagtaaaaaa atattcagat
    47701 atagataaag gtataagtga acataaaata aatattttaa ttggtcttga aggattaagc
    47761 catattggaa gtaatataga ttcaatagat tatttttata aagaagttgg tgcaagacat
    47821 gcatcattaa cttggaatga acagaatgaa cttgctacag gttgtttagg agatgaaaat
    47881 aggggtttaa ctgaaatagg taaaaaagca gtccataaaa tggaaaattt gggcatgata
    47941 gtagatgtgt cacatttaaa tgaaaaatca ttttgggatt taatgtctat tgcaagtaag
    48001 ccagttatag cttctcattc taatgctaag agtttatgtg atgttaaaag aaatcttaca
    48061 gataatcaat taaaagaaat tgcaaataca ggtggattag ttggaataaa tagttttaga
    48121 ggatttacta atagtaatcc taagaagcaa gatttaatac atttggttga ccatatagat
    48181 tatatggtaa atcttatagg tatagaacat gtatcttttg gttttgattt tagtgattac
    48241 ttggggaaag agaccatagg ttctttcact ggaaactcta gtatgccaag tgtagatggt
    48301 cttgaaagca cacatacagc acataacata gtagtagagc ttgaaaatag aggttataaa
    48361 aaaagagata tagatttaat ctctagtggt aatttttata gactcttt
//
LOCUS       DAMJVM010000021        44678 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.021, whole genome shotgun sequence.
ACCESSION   DAMJVM010000021 DAMJVM010000000
VERSION     DAMJVM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44678
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.021"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            180..1013
                     /locus_tag="PC523_001272"
     CDS             180..1013
                     /locus_tag="PC523_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HDF2474816.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFVVVGARVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTATEVSKGPSKSSGKVAYITIDDGPSKFTDQIIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIIQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1106..2038
                     /locus_tag="PC523_001273"
     CDS             1106..2038
                     /locus_tag="PC523_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2474817.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKEYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEISASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFSKDDKNAEFTKEVFKLKKNEVSKVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2090..2572
                     /locus_tag="PC523_001274"
     CDS             2090..2572
                     /locus_tag="PC523_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2474818.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILIRTSIDFIRKNRKYNNMTDIDLVDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2584..3453
                     /locus_tag="PC523_001275"
     CDS             2584..3453
                     /locus_tag="PC523_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2474819.1"
                     /translation="MTSKERLNKLKEEYHKIPIPKKLDTIINHEKIENIYQEKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFEERMEKIQSSKNKGHKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKRDSTKFYFIDQKKELPVEDFKTINKYQDFYFNKNEELVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3681..4244
                     /locus_tag="PC523_001276"
                     /pseudo
     CDS             3681..4244
                     /locus_tag="PC523_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429797.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
     gene            4286..5107
                     /gene="xdhC"
                     /locus_tag="PC523_001277"
     CDS             4286..5107
                     /gene="xdhC"
                     /locus_tag="PC523_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HDF2474820.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVIVLDSFEEIPDFPTDENSYIIILTRGHLYDSSALEWALKRDAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSRVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5112..5873
                     /locus_tag="PC523_001278"
     CDS             5112..5873
                     /locus_tag="PC523_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDF2474821.1"
                     /translation="MNIGSKDSDILNVNFRGIDIKVILVYKNRKNISIKIDPFGKVIV
                     MSPPKMSKKIIKDIIIEKGDWILKKSEKYKGREEVYEQRMFITGEKFLYLGEEYHLVI
                     KEMLKDSVKKSNCKITINEYEIIVQTNDTSTDFIKKSLKSWYKIESERLVLERIDFLK
                     LNYEIMNQLVPASVKVKEQKKRWGSCTSQKNIYINSKISMAKLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKITI"
     misc_binding    6034..6290
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6403..6642
                     /locus_tag="PC523_001279"
     CDS             6403..6642
                     /locus_tag="PC523_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2474822.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMKH"
     gene            6921..7916
                     /gene="ilvC"
                     /locus_tag="PC523_001280"
     CDS             6921..7916
                     /gene="ilvC"
                     /locus_tag="PC523_001280"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDF2474823.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVY
                     VVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLIMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            7969..9657
                     /gene="ilvB"
                     /locus_tag="PC523_001281"
     CDS             7969..9657
                     /gene="ilvB"
                     /locus_tag="PC523_001281"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HDF2474824.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFKVANSGRKGPVLVDVP
                     KDLFLAEMDFSDEDYDLCQIDDYIDYKSDFSLDDEINVKLLNEAIDIIKESKKPVIYA
                     GGGVKSSDSEDILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLIGDVKLVLS
                     LLIERVESKNNSNWKEEIKTFRKSEGIQTDEFHPQSILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGAKVGNIDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNERYSQTDINENVDYVKLVNAY
                     KIDGYKISNMEELGKVLDVIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            9865..10542
                     /locus_tag="PC523_001282"
     CDS             9865..10542
                     /locus_tag="PC523_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2474825.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDVNPPS
                     FEKPLKK"
     gene            10712..12748
                     /locus_tag="PC523_001283"
     CDS             10712..12748
                     /locus_tag="PC523_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HDF2474826.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNEEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITNKELYKEIAMKTIDYVVREMQDKDGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGTFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIDNLSKKV
                     FEYRKERTSLHKDDKILTSWNALMVVALTKAYSTLKNDMYLDYSNKCLDFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCIDLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNKLEEMSYKQ
                     LKLYVNNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLSAFKELISENFIPNTT
                     FLAKKYNEENTIIGFLNNYKLKEDKTSYYVCQSNSCSQPINNLQKLKDMILGVE"
     gene            complement(12955..13502)
                     /locus_tag="PC523_001284"
                     /pseudo
     CDS             complement(12955..13502)
                     /locus_tag="PC523_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
     gene            13784..14719
                     /locus_tag="PC523_001285"
     CDS             13784..14719
                     /locus_tag="PC523_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HDF2474827.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKNPKAVVQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGRQGIILNLAHMIINREI
                     KKYGRRSRSNKINDLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLIQGLKEIEDKENLKKVPLDVPIYIISGDKDPIGKNSKGVLRLRDRYIKLGVKDVT
                     CKLYKGGRHELLNEINKEEVFEDVICWLNNKIGIL"
     gene            15083..15850
                     /locus_tag="PC523_001286"
     CDS             15083..15850
                     /locus_tag="PC523_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2474828.1"
                     /translation="MSKILRVKNIKKYYKSRGNITKAIDNISFEVESGEYIGIMGASG
                     SGKTTLLNCISTIDKTTDGNIYIGNKDITTMNEDELSKFRREELGFIFQDFNLLDTLT
                     AHDNIALSLSINGVNSKEIDNKIKKIANKLGVYEILNKYPYEMSGGQKQRIACARAII
                     TNPSLILADEPTGALDSKSAKLFLENLEVLNRELKATILMVTHDAFTASYCKRILFIK
                     DGKIFNELVRGNNTRQEFFDRIIKVISVLGGDDKHVF"
     gene            15831..17960
                     /locus_tag="PC523_001287"
     CDS             15831..17960
                     /locus_tag="PC523_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2474829.1"
                     /translation="MINMFFKKNKSRRSRNKRKNNYVSRVSSESFKKLALNNVKKSLK
                     NYLIYFITLVFGVIILYTFNSLDNDIAILSSNALLSSSISFVRGIVSIFSILVCIIFA
                     FLIIYANNYLMKQRKKEFGIYSILGMDRRDINKLMFREMMIIGIFSLIIGIIFGVFVS
                     KGLSIFALKMLGISSSGFSFSMLTTIKTIVFFGIVLLLVNKFNKKTIKKYKLIDLLNA
                     NKKNEVSVAKGKKSNLILFFLSIVAIVLAYIIPLKMKISTFILGICLILILVGIYLFF
                     MSVSDFIMLKIKKHRFIYYKNLNPFIVSQISSQIKTMSMSITMICMLLFSSLIVIPFG
                     ISFGKYLIDDLQEATPYDVTLSKLYMNGDNIVEYSTKKNDIDSKTPSLKDELIENGFP
                     LTSFSKSTSDLRIYNLKNISQYDFISNDKDRTNELKDNKLHIVSISDYNFTRKQSGLK
                     EINLKDNEFAINCASEDYRPFYEKYIKSQPQNYITVNGVKLKLNQTSLYTNSYNTNTV
                     PLDLGTIIVPDAVVKDLNPIMTTLNIDYKERSSNLENKFMDSYYKFEEKINNSEAYSI
                     LYKSVIDGEKRALNTSFSFVAIYIGIILLISAGAILALQQLIESTVNKERFKLLSKLG
                     VNKKDMKKAIFIQVSILFAVPLILALINALFISKVFFVIMPFIAETGIITTMLLTLGI
                     VIVVYGIYFISSLLESLNIVNDSKEII"
     gene            18177..19388
                     /locus_tag="PC523_001288"
     CDS             18177..19388
                     /locus_tag="PC523_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2474830.1"
                     /translation="MQIKIGKIIQALRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVTKIYKELESIFAGIEVDLSTEEPKEEC
                     KRDLEYVKNLSNMYIEKYPKSYLLKLKICSLYQMYSYKFGKNDFNNNVRETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKPIGDTSVNLAMIYLNQNKL
                     EEAEILLQNKLFSNVFNLSLDCKGIINVYKYQYKELKKKLEYGDSNKKAIEKEMEYIK
                     NKLLGYANLSLEIKKMISEDKGAFFGIYMDYMELSLTFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVKSKNSYTFNMYTNLLIMFSDDSYNELRGEPIFESIIEK
                     ILELEKLLKDK"
     gene            complement(19699..20220)
                     /locus_tag="PC523_001289"
     CDS             complement(19699..20220)
                     /locus_tag="PC523_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDF2474831.1"
                     /translation="MKFERLRTSKDKLYYKAMELYKTSFPFHEQRKSSLQAKIMENKE
                     YQFNLIYDKNQFVGIILCWETKSFIYVEHFCIFPEMRNNRYGQKTLELLNKRGKTIIL
                     EIDPPIDELSTRRKKFYERVKYKTNYYKHIHPPYQEKFNGHQLVVMSYPERLSKMEYD
                     KFNQYLKNHVMNF"
     gene            20582..21379
                     /locus_tag="PC523_001290"
     CDS             20582..21379
                     /locus_tag="PC523_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDF2474832.1"
                     /translation="MEKREKRIIISSLLSVSILMSLVSIYSILNKEDISLTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGKEGEKKLIYNLTYHDEKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGSKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            21489..22079
                     /locus_tag="PC523_001291"
     CDS             21489..22079
                     /locus_tag="PC523_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDF2474833.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLVRFNEDVVKNSPDIVFIMGGTNDFIAGAGNEVINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNIREDWSCFTDFNIVSQKLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            22144..23841
                     /locus_tag="PC523_001292"
     CDS             22144..23841
                     /locus_tag="PC523_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDF2474834.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKIELEKIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETNRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSIIPTPSEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNIPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTIGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYEGLIELYEK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSVKVIDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVGKLK"
     gene            24042..24881
                     /locus_tag="PC523_001293"
     CDS             24042..24881
                     /locus_tag="PC523_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDF2474835.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLIGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIKHE
                     MLK"
     gene            25400..27256
                     /locus_tag="PC523_001294"
     CDS             25400..27256
                     /locus_tag="PC523_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HDF2474836.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYQVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIVGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGESTGKLFKDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIIDNIIYSGYSNKNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVESKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTAKDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKIQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKEDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGVGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    27339..27579
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    27645..27880
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            27965..29182
                     /locus_tag="PC523_001295"
     CDS             27965..29182
                     /locus_tag="PC523_001295"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDF2474837.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKGKKYSNMITNNIDDIFNKDIDILVEVMGGLEPALSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            29298..29792
                     /locus_tag="PC523_001296"
     CDS             29298..29792
                     /locus_tag="PC523_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474838.1"
                     /translation="MENKKYANGGYSEDWYERGESTAKWFQNDREEYERAAYDEDRER
                     RGSNCGGSDSEGNRPRNCERFRREAEIREREAREAFCESSERKKEALAYECEARKLWE
                     QAEKYWDEYSKYNYQGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCN
                     CCRK"
     gene            30031..31296
                     /gene="hisD"
                     /locus_tag="PC523_001297"
     CDS             30031..31296
                     /gene="hisD"
                     /locus_tag="PC523_001297"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDF2474839.1"
                     /translation="MKRIKTSSMKEKVDEKILREQVSSIIEDIRNNKDIALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIKEFAKAQKSSFENSFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKIKPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSKPVYIAADLLAQCEHDLNARGILLTNSLEIAEEVEKNI
                     ETMLKDLPTKDIAYSSWKNNGEIILVDDMEEAIKISNFYAPEHLEIAVNKCDDIFDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEEAI
                     ESLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(31501..32253)
                     /locus_tag="PC523_001298"
     CDS             complement(31501..32253)
                     /locus_tag="PC523_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2474840.1"
                     /translation="MSYFQYQGNACFYEEYGQGKPIVFLHGNTASSNMFKALMPLYVE
                     NFRCILIDFLGNGQSDRVSQFSPDMWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCQGKDWEN
                     VVNLDTKALLQCAKEKRPLFHKELCKLEMPILFIGSKEDEMCRHNLEEEYKQMATFIR
                     KASIHIFSHGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(32371..32640)
                     /locus_tag="PC523_001299"
                     /pseudo
     CDS             complement(32371..32640)
                     /locus_tag="PC523_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            33115..33889
                     /locus_tag="PC523_001300"
                     /pseudo
     CDS             33115..33889
                     /locus_tag="PC523_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            33884..34089
                     /locus_tag="PC523_001301"
                     /pseudo
     CDS             33884..34089
                     /locus_tag="PC523_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
     gene            34495..35127
                     /locus_tag="PC523_001302"
     CDS             34495..35127
                     /locus_tag="PC523_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2474841.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            35538..35630
                     /gene="kdpF"
                     /locus_tag="PC523_001303"
     CDS             35538..35630
                     /gene="kdpF"
                     /locus_tag="PC523_001303"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDF2474842.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            35671..37353
                     /gene="kdpA"
                     /locus_tag="PC523_001304"
     CDS             35671..37353
                     /gene="kdpA"
                     /locus_tag="PC523_001304"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDF2474843.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYSAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGIILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGYMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTAVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAVPAGLEGISNPGF
                     HGLSQVLYEFSSSAANNGSGFEGLGDNTMFWNMTTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            37378..39435
                     /gene="kdpB"
                     /locus_tag="PC523_001305"
     CDS             37378..39435
                     /gene="kdpB"
                     /locus_tag="PC523_001305"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDF2474844.1"
                     /translation="MDTKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRVYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFITVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGIVPEDLEENVNRVAKLGGTPLVVCVDNGIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQSE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            39454..40080
                     /locus_tag="PC523_001306"
     CDS             39454..40080
                     /locus_tag="PC523_001306"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDF2474845.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELRDRVQKDIEQFLKDNPTVKKEDIPTDLLTASGSGLDPNISPDSAKIQIPAV
                     AKASGISESKLQKIVDDNTSKKVFGVLGEDRVNVLKVNVEVARILGLI"
     gene            40317..41225
                     /gene="cysK"
                     /locus_tag="PC523_001307"
     CDS             40317..41225
                     /gene="cysK"
                     /locus_tag="PC523_001307"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDF2474846.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEKRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSIER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIKVVALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEIILVDDEDAFDTVKLLASKEGILVGISSGANIFGAIEM
                     AKRYPDKKIVTVAPDGVDKYMSMGIF"
     gene            41252..41842
                     /gene="cysE"
                     /locus_tag="PC523_001308"
     CDS             41252..41842
                     /gene="cysE"
                     /locus_tag="PC523_001308"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDF2474847.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVDYEYDELDNVYYI"
     gene            42003..42191
                     /locus_tag="PC523_001309"
     CDS             42003..42191
                     /locus_tag="PC523_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDF2474848.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMNDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(42245..43114)
                     /locus_tag="PC523_001310"
     CDS             complement(42245..43114)
                     /locus_tag="PC523_001310"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HDF2474849.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEVGISTEDINKLVHEFTLQNGAIPAPLGYNG
                     FPKSVCTSINNEVCHGIPNKDIILKDGDIINVDVSTIFNGYYSDASRMFMVGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAVNSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(43311..44561)
                     /locus_tag="PC523_001311"
     CDS             complement(43311..44561)
                     /locus_tag="PC523_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474850.1"
                     /translation="MIMENNLNTAKRNNTELSQNDSLKVNLQIGVDFTLLSKSLKAFY
                     KKEKDFYCIALAPCNVSSEEANRKISIYDMIKEINILIESITDKNELLSEELFTNNLS
                     EFYDESLDEIYIDLKQAYLYIKKNTNGLNIEDSIEYVFDVDISNDIKAKENPLINFNS
                     LSLVVWSGETKHILQQMNILNLEDIHSYSMENNTNITIYENDNLQYKFDEESEDIKDS
                     NIDIIEQMLILPDDLNYDKDEVENMKSRLAKINIKYLQALKEKDIKIKLINSNLTDEP
                     EFSDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYTNVHSSKNLELHETA
                     HAIDKNVLNKKSNSEEFMKVFAQERYKLYDPKQIAHAYISKFIEEFFAESFVHYYLDE
                     DSKNTLKENCPLTYDFLEKLELYY"
BASE COUNT        17750 a         4642 c         7632 g        14654 t
ORIGIN      
        1 acattctata caattataca taaattatac agcataataa tcatcaaaat aacaaatttt
       61 aaaaattaag caaaactttt tgtcgaagtt caacgtctat atatatgtag tgtaaatttt
      121 tggaataaaa tactacaaaa atataaatgt aaaaatatag taatattgga ggtcgaacga
      181 tgagaaataa aaagaaaaaa ataaatagaa agaagttgta tttgctttta agtgctattg
      241 tagtgtgtct ggcttttgta gtagttggtg cacgcgttag ctctttaaat atgaaagaaa
      301 atgaaattag tagaaatgca aaactgtcaa gttctactat tacagatata gtatctaata
      361 cagcaacaga agttagtaaa gggccaagta aaagttctgg aaaagtagca tacataacta
      421 tagatgatgg tccatctaaa tttactgacc agattataaa aactttaaat aaatataatg
      481 ttaaagcaac tttttttatg atagatggaa atatgaagga atatccacag caagttaaaa
      541 atataattaa aaatgggaat acagcaggtt ttcatagtgt atcacatgat attcacaagc
      601 tttatgttac tagtacatca gcaaaagaag aatttgatac aaatgatcaa actttttata
      661 agataactgg caaacattca aaagtaataa gaatacctta tggaagcaaa ccatatacac
      721 cacaagcttc ttatcaggct ttagttgatg ctggatataa aatttgggac tgggacttag
      781 atacagaaga ctggagatct aattcttctc aaattataca aaatgtaaaa aatcatatta
      841 agaatagaaa aggtgaagat aaagaccaac ttgtagtttt aatgcatgag aaaaaacaga
      901 gtgcagaagc tttagattca gttttaaaat ttttatcaga tgaaggttat gaatttgctc
      961 cagtagatca aaatcaaata ccaaaaaatt attggttacg taatttagag tagttaaaat
     1021 ataagaatat taattgatat ttttagaaat tgagaataaa aatgagtgag gtgtaggtat
     1081 attgaataaa aaactttata taggaatggt cggaatattg tctttgatga tggttggttg
     1141 taataaaagt ctagcaaaag taaatgatgt ggaaattaca aaggaacaat acaaaaaaac
     1201 aaaagcagtc ttatctgcta ctaataatta tataaatggg cagtctttag atgaattaga
     1261 aaaaactttg gataaaaaag gtagaaataa attagaaaat gttataatat cgtttatggt
     1321 ggataatgaa cttttatatc aagaggctaa ggataaagga ctaactccaa gtaaaagtga
     1381 agtagactca aaatatcaag aactagaaga taagatgaat ttgaatacaa gctataagga
     1441 aaaaatggac aaagcaggtg ttgataaaga atatttaaaa caagaaatat caagagattt
     1501 agccatagat aaaaataaaa aagcctttga agatagaata aacataagtg ataatgatat
     1561 ggaagcctac tatacaagtc ataaaaaaga ttttaatgta gaagaaataa gtgcttctca
     1621 gatacttata tcgactttag acaaaaataa aaaagaagta agtaaagata aaaaagaagc
     1681 tttgaagaaa aaggcagata atatattgac taaaatcaaa aatggagaaa gttttgaaag
     1741 tttggctaaa aaatattcag atgataaagc aactggaaaa aatggaggac agctaggata
     1801 tttttcaaag gatgacaaaa atgctgagtt tacaaaggaa gtgttcaaat taaagaaaaa
     1861 tgaagtttca aaggtgtttg aaactagtta tggatatcat atagtcaaag ttactgataa
     1921 aagagaaaga caaaaaagtt ttaatgagtg tcagagttta ataagagaat ctattttaaa
     1981 tgaaaagtat attgagcata taaaaaaatt aaatgaagat gctaaaatag acagatagaa
     2041 tttctaacag ctcaatcctg gcctgtaatc ggagggagat tttacaggaa tgtttaagag
     2101 tttaaagaga acgaaagttg agaaatacat tatcgataat aaagactctt tttatagaat
     2161 agcatatagt tatacgaaga atgaggaaga tgccttagat gtggttcaag aagctatgta
     2221 taaggcacta tactcagtgg aaaatattaa agaagtaaat tatataaaga catggtttta
     2281 taaaatttta attagaacga gtatagattt tattagaaaa aaccgtaaat ataataatat
     2341 gacagatatt gaccttgtag atgagactgg agagtatgat aaatatactg atttggattt
     2401 aagaagagct ttagaagaat tacctattga atacaaatct attataatat taagattttt
     2461 tgaagattta aagattgaag aagtagcaat aatattggat gaaaatgtga atactgttaa
     2521 aactaggtta tatactgctt taaaaaaact aaaattaaaa attgaagagt agggataaaa
     2581 agaatgacaa gcaaagaaag gttaaataag ttaaaagagg agtatcataa aattccaata
     2641 cccaagaaac tagatactat aataaatcat gaaaaaatag aaaatatata tcaagagaaa
     2701 aataaaaaag taaatagatt aagatttaaa gttgcaatag cctttgcatg tatatttaca
     2761 gtattagtaa atataagtcc tgtatttgca gacaattttt ctaaaatacc tgtaattgga
     2821 gcaatagtag aagtgataac tataaaaaat tatagtttaa aaagtgaaaa ttatgaagct
     2881 gagattgata ttcctaaaat acgaggttta aaagataaaa acttagaaca aagattaaat
     2941 agtagtttta tggaagatgg aaaaagactt tatcatcaat ttgaagagag aatggagaaa
     3001 atacaatcat caaagaataa gggacataaa tctttaagtt tgagttatag tgtaaaaaat
     3061 aattctaaaa aatttctatc aatagagatg acaaaaaatg agatagaggc ttcatcatat
     3121 gtgagtaagg tacattatac tattgataaa aaaagacaaa tagtcttgac tttgccaatg
     3181 ctttttaaag atgataagta tataaaagtt atcagtgata atataaaaga acaaatgaga
     3241 gaacaaatga aaagggatag tacaaaattc tattttatag atcaaaaaaa agaattacct
     3301 gttgaagatt ttaaaacaat taataaatat caggattttt attttaataa aaatgaagaa
     3361 cttgtaattt gttttgacga atatgaagta gcacctggtt atatgggtgc agttgagttt
     3421 gtaataccat ataaagtaat aaaagatttg taaattaata gtagtttata tataaatatt
     3481 ttaaggagtg caatataact acatataaaa aacttaaaaa atctatattt aaaattgcta
     3541 tagtttcaat atgtttatta gtatcattag taattacaaa ccctatattt tcagataatt
     3601 tttctaatga gacttaaact agtgatatga taaaggtgtt acatgctaag agtttcaact
     3661 ttaaacaaga caattatgaa gtgaaggtta gtattcctaa aataaaaggt ttaaaagata
     3721 aaaatttaga gattaaatta aataatgaat ttttataata tgggataaaa atttacagtg
     3781 aacttcaaaa taaaataaaa aaataataag gggtatagat acctaaatgt aagctataat
     3841 gtcaagaata attctgagga ctttttatca atagaaatga caaaagaata gatagaagcc
     3901 tcctcatctg taactaagaa gcattacact atcaataaaa atagagaaat actattaatt
     3961 taccaatatt ttttaaagat agtagatata ttagagttat aagtgataat ataaaagaac
     4021 agatgagaaa ttacattaag aaagataata caaaatctta ttttatagac caagataaag
     4081 gttccctagt gaaagagttt gaatgtataa ataagtgtca agatttttat tttaatagaa
     4141 atggaaatct tgtaattagt tttggtaaat atgaattagc gcctggttat atgagagcag
     4201 tagaatttgt gataccaaat gaagtaataa aaagcctaag atagatatat agaagatttt
     4261 tagatagact ggtaaggaga attatatgaa tatatatgaa caagcaataa gattaataga
     4321 aaataatgag gattttgcat ttgctacaat aacttctcat tcaggttcaa caccacgtga
     4381 aactggagca atgatgattg taaaaaatga ttctactata tttggttcag tgggaggagg
     4441 tagtgtagaa gcagaatgta taaaacatgc aattaatgtt ataaaaaata gagaatcgat
     4501 gctgtataaa tttactctaa acaaatctga tgtggcaaaa ctaggaatga tatgtggggg
     4561 tactggagaa atacaaatag attttataga tagtaaatta aaaagtaata tagaaaaatt
     4621 caataagaga ctaaaggaaa atacgagtaa agcgtatata tttggagcag gtcatatttc
     4681 aagagatgtt gctgtgatac tatctctttt agaatttaga acagtagtaa tagatgatag
     4741 agaagaattt gctaatcatg aaaggttccc agaatcagaa gtaatagttt tagattcttt
     4801 tgaagagata ccagattttc caactgatga aaatagctat ataattattt taacaagagg
     4861 tcatttatat gattcaagtg ctttagaatg ggctcttaaa agagatgctg gttatatagg
     4921 tatgattgga agtagaacaa aaattggatt gacgtacgaa aaactcatga aaaaaggttt
     4981 taagaaggaa gaattatcaa gagtacatgc accaataggc ataaaattga atgctcaaac
     5041 accagctgaa atagctgtat gtatagctgc tgagcttata aactgtagag ctaataagga
     5101 aaaataaact aatgaacata ggaagcaaag atagtgatat tttaaatgtt aattttagag
     5161 ggattgatat aaaagtaatt ttagtatata aaaatagaaa aaatatttct ataaaaatag
     5221 atccatttgg aaaagtaatt gtgatgagcc ctcctaaaat gtctaaaaaa ataataaaag
     5281 atataatcat tgaaaaagga gactggatac ttaaaaagtc agaaaagtat aaaggaagag
     5341 aagaagtata cgaacaaaga atgtttataa ctggcgaaaa atttctttat ttaggtgaag
     5401 agtaccatct agttataaaa gaaatgttga aagattctgt taaaaaaagt aactgtaaaa
     5461 taactattaa cgaatatgaa ataatcgttc agacaaatga tacaagtact gattttatta
     5521 aaaaatcttt aaagtcttgg tataagatag aaagtgaaag attagtgtta gagagaatag
     5581 attttcttaa attaaattat gaaataatga atcaattagt tccagcaagt gttaaggtaa
     5641 aagaacaaaa aaagagatgg ggaagttgca catctcaaaa aaatatttat ataaactcaa
     5701 aaatatctat ggcaaaatta gatgtaattg attatgtaat aatacatgag tttagccatt
     5761 tagtacatat gaatcactct aaaaaattct atgatttagt aaaatctatt atgccagatt
     5821 ataaagacaa agaaaattgg ttaaaaataa atggatataa aataacaatc tgaaaacggt
     5881 tattccaaaa cataggtgta aaatgtaaga gaagtattat tgtgaaaaaa ataaataatc
     5941 tgaaaatttt aaaaaaagtc gttgacattg taaatgatag ggaatataat ctatattaac
     6001 aacaaaacag aactgaaata taaaattaaa aaattaaaag caatgacaga gacaacaata
     6061 ctaatagtgt tatccacaga gaaccaatgt tgctgagaat tggtgttaac ggtagtataa
     6121 tgatcactct ggagcttttg tactgaatat ttttagtaag tacaaacgtc tgtcaacgtt
     6181 acatgactaa ggttaaggta attaattatc cgacctgaaa aaagaggggt agatgtattt
     6241 ttatatctac aaataaggtg gtaccgcgga ataaaacttt cgtccttata agtaatattt
     6301 atattgctta taaagatgaa agttttttta ttttaagtga tataaataaa tttaatccat
     6361 ataaaattaa taaaaaaata ataaaaacaa ggaggaattt ttatggaaaa tactgtatat
     6421 gctaaattaa accaaggaat agattctttt ttaagagtgg ctatgactct aagaagaaga
     6481 gaagttgata tacaatcaat aagcatgact gtagataata tgaataactc aggtataaaa
     6541 ttaatagtaa atgaagaaaa gacatcttta gattctgtct taaaccacat gaaaaaacta
     6601 catgatataa gagagataac tgttgaaaga atgaaacatt aattaaatat atttttataa
     6661 agctaaagat aaatatgctt attttaaaat aaaaaactaa agataaatat taaataagaa
     6721 aaatcttaga ttaaagtaca agtatcattg aatttgtcag tttatcatat ctgataaaaa
     6781 ctgaaaattc taaaaatata attgtaaaac tatataaatt tagaatttaa atctgttgaa
     6841 taatattaaa ataatgataa tgagtatgaa tttctaataa aaataaatat cataaaaata
     6901 agaaaaagta ttgggaggaa atgaaaatgg caagaatgta ttatgaaaaa gatgtggatt
     6961 tagaggtttt aaaaaataaa aaggttgcag ttttaggtta tggaagccaa ggacatgcac
     7021 atgcacaaaa tcttagagat aatggtgtat atgtagtgat aggtctttat gacggaagta
     7081 aatcagcaca aaaagcaaaa gaagatggat ttgaagttaa gagtgtagca gaagctacaa
     7141 aagaaagtga tttgataatg atgttaatgc ctgatgaaaa acaaaagaaa gtttatgaag
     7201 agagtgtaaa ggataactta aaagaaggtc agacattagc ttttgctcat ggattcaata
     7261 tacattataa ccaagtacaa ccaccagaat ttgttgatgt tgtaatggta gctcctaaag
     7321 gacctggtca cttagttaga aatgtattta caaagggaag tggagttcct gcactatttg
     7381 cagtatatca agactacact aaaaaagcta cagagacagt tttagcatat gcaaagggga
     7441 taggagcaac aagagcaggt gttttagaaa ctacttttaa agaagaaaca gaaacagatt
     7501 tatttggaga acaatcagta ctttgtggtg gaataagtga acttataaaa ttgggatata
     7561 aaacgcttgt agatgcaggt tatcaaaaag aagtagcata ctttgaatgt ttacatgaaa
     7621 tgaaactaat agtcgacctt atatatgaag gtggatttga aagaatgaga tatagcataa
     7681 gtgacacagc agaatatgga gattatgtat ctggtaaaag ggtaataact gatgccgcaa
     7741 aacaaggtat gcaaaatgtt ttagaggata tacaaaatgg aaaatttgca aaagcgtgga
     7801 ttaaagagaa tgaagaagga cgtgagaact tcctaaaaac tagagaagaa gaatataata
     7861 cagaaattgc cgaagttggt agaaatctaa gaagtatgat gtcattctta aaataaatat
     7921 atattaaatg gattagtttg agggaaagag tggtgtatag tagtgcgtat gaatggagca
     7981 aaggtaattt tagaatgttt aaagaaagag ggcattgaca caatatttgg atatcctggt
     8041 ggagcagtaa tacctcttta tgatgctcta tacgattatt cagatgattt taaacatata
     8101 aggacatctc atgaacaagg tttagttcat gccgcagatg ggtacgcaag aagtactaat
     8161 acagtaggag tttgttttac aacgtctggt ccaggagcaa caaatgccat aactggaata
     8221 gcaacagctt ttatggattc ttcacctatg gttgtaatct caggtcaggt tccaacaagc
     8281 ttattaggta aagattcttt tcaagaaatt gacataactg gagcaacatt atctatgaca
     8341 aaacataatt atttagttag aaacactaaa gaattagttc ctacaataaa agaagctttt
     8401 aaagttgcaa attcaggtag aaaaggacct gtacttgtag atgttccaaa ggatttattt
     8461 ttagcagaaa tggattttag tgatgaagat tatgatttat gtcaaataga tgattatata
     8521 gattataaga gtgattttag tttagatgat gaaattaatg tcaagctttt aaatgaagca
     8581 atagatatca taaaagaatc taaaaagcct gtaatatatg ctggtggagg agttaagtct
     8641 tcagatagcg aggatattct tgaaaaattt gcaactaaga tagatacacc tgtactaaat
     8701 acacttatgg gtcttggtaa tatagataga aaaaatgaac tatctcttgg aatggttgga
     8761 atgcatggaa gtagagaaag taatttagcc ctttctaatt cagatttagt gatagcgata
     8821 ggtgctagat ttagtgatag agtaataagt aaaagctctg aatttgctaa aaatgcaaaa
     8881 ataatacaca tagatataga cccatctgaa ataagtaaaa atatagaatc taatgtatct
     8941 ttgattggag atgtaaagtt agtccttagc ttactaattg aaagggtcga aagtaagaat
     9001 aatagcaatt ggaaagaaga gataaagaca tttagaaaga gtgagggtat acaaacggat
     9061 gaatttcatc cacaaagtat acttaaaaaa ataaatgaga aatatgaaac attaaagaag
     9121 cctactgtgg tagtaactga tgttggtcaa catcaaatgt gggctgctaa atactggaat
     9181 tttaaaggaa ataaaagttt tataacatca gcaggattag gaactatggg atttggttta
     9241 ggtgcagcta ttggagctaa agttggtaat attgataaaa atgtggtttt agtaactggt
     9301 gatggaagtt ttagaatgaa ctgtaatgaa ctagctacag ttgcaaatta taatgtacct
     9361 atgcttattt tactactcaa taatagaacc ttaggaatgg taagacaatg gcagaagtta
     9421 ttttccaatg aaagatactc tcaaactgat attaatgaaa atgtggatta tgttaaactc
     9481 gtaaatgcat ataagattga tggatataag atatccaata tggaagaatt aggaaaggta
     9541 ctagatgtga tagattttaa taaacctgtg tttttacaat gtgatataga taaggattat
     9601 gatgtttatc ctattgtagc acctaatgat gcattagaaa atttgatatg taactaaaac
     9661 ttatactgaa tattgataaa attttttatt aaacttttat tgatattcag tttttattta
     9721 gagatagagt ggtatttttt atttttgtag ataaaaaaat gaatatataa ttaagaattt
     9781 atcaaatttt aattatacaa tttagaataa tattacaact taagtaaata ataattttga
     9841 ataataattt acggaggtat tcatatgttt aaacacgata aagcattatt aaaagaagta
     9901 aaagtagaaa gaccaaatcc tcaatatgct gttttaatgc aagaacagtt gggtggtgca
     9961 aatggagagt taaaagcagc aatgcaatac ttgtcacaaa gttttagaat aaaagaccct
    10021 caaataaaag atttatttct tgatatagca gcagaagagc ttagccatat ggaaatggta
    10081 gcacaaacta taaatttatt aaatggacat gatgttgatt ataatgcagt taatacaggc
    10141 gaaatagaaa cacatgtatt aactggtttg tcaccagttt taataaactc atcaggagct
    10201 ccttggactg ctaattatgt tacagttaca ggggatttag tagctgactt attatcaaat
    10261 atagcttctg aacaaagagc taaggttgtt tatgagtact tgtatagaca aatagatgat
    10321 aaatatgtaa aagaaacaat agatttctta cttaatagag aagaagcaca taatgctcta
    10381 tttagagatg ctttaaataa agttaaagat acaggttcaa atagagattt tggagttaca
    10441 gaagattcta agttatattt cgatttatct actccagggc caaatcatga tactaagata
    10501 gatgttaatc ctccttcttt tgaaaaacct ttaaaaaaat aattttatat tttttatgtt
    10561 attttagtag tcataagaaa ttatttacta gaagagttta ttagataaaa aattaaatat
    10621 gtatagttaa aaatgggtat acacttacta taagtgtata tctatttgtt tattgcaaaa
    10681 catataataa aataacaaat gaggtggtat tttggacagt aatagaaaac ctaataatct
    10741 aataaatgaa aaatctccat atcttttaca gcatgcatat aatcctataa actggtatag
    10801 ttggaatgaa gaggctttta aaaaagcaaa agaagaggat aaaccaatat ttttaagtgt
    10861 aggatattca acatgtcatt ggtgtcatgt tatggaaaaa gaatcttttg aagatgaaga
    10921 agttgctgaa ataatgaata gaaattttgt tgcaataaaa gtagataaag aggaaagacc
    10981 tgatgtagat agtgtgtata tgacagtttg ccaagctatg acaggtagtg gtggatggcc
    11041 aatgactata ataatgacgc cagataaaaa accttttttt gcaggaacat acttcccaaa
    11101 atattcaagg tataatagac ctggagttat agatttatta gagaatgtat ctgaaaaatg
    11161 gaatacaagt agagatatac taataaaatc tggagatgaa ataatagaag ctcttaagga
    11221 tgattttggt gtaaaaaata ctgaaggtga tttatcaaaa gagatgttaa gttcatcagt
    11281 tagagtattt aaggctattt atgatgaaaa gtatggtggt tttggtaatg cccctaaatt
    11341 tccaagtcct cagaatctta tgtttttgat gaaatactat agtatagaaa aagataaaga
    11401 tgtattaaaa atggtagaaa aaactttaga tggtatgtat agaggaggat tgtttgatca
    11461 tattggattt gggttttcaa gatactctac tgataaaaag tggttggctc ctcattttga
    11521 aaaaatgcta tatgataatg ctatgcttac tatagctttt ctagatgcgt ataaaataac
    11581 aaataaagag ttatataaag aaattgctat gaaaactata gattatgtgg ttagagagat
    11641 gcaggataaa gatggtgggt tttattcagc gcaggatgct gatagtgaag gtgaagaagg
    11701 taaattttat acatttaatc ctcttgagat aattgaagtg ttgggtgaag aagatggaac
    11761 tttctttaat aattattttg atataactag tagtggaaac tttgagggca aaagtattcc
    11821 taatcttata aaaaataaag aatatgagag acataatgaa aagatagata accttagtaa
    11881 aaaggtattt gaatatagaa aagaaagaac atctttgcat aaagatgaca agatacttac
    11941 atcatggaat gcattgatgg tagttgcttt aacaaaggca tatagtactt taaaaaatga
    12001 tatgtattta gattattcaa ataagtgcct tgattttata aataataatc ttgtaaatga
    12061 gtcaggtaga ttgttagcta gatatagaga tggaagttcc gactatttag catatttaga
    12121 tgattatgct tttttaatct gggcgtatat agagctttat gagtcaacat ttaatatgaa
    12181 atatttggaa aaggctttaa atttaaatga aagctgtatc gatctttttt gggattatga
    12241 aaaaagtgga ttttatatat atggtaaaga tagtgaaaat ttaatagcaa gaccaaaaga
    12301 tttgtatgat ggagctattc catcaggaaa ctctgttcaa ttatacaatc ttataagact
    12361 tgcgaaaatt actggcgata ataaacttga agaaatgtca tataaacaat taaaactgta
    12421 tgtgaataat gtaaagagtt ctccaactgg ctacagcttt tatatgcttt cattgatgtt
    12481 tgaactttat tctactaagg aaattatatg tatttttaag gaagattccg atttaagtgc
    12541 atttaaagaa ttgataagtg aaaattttat accaaataca acttttttag caaagaaata
    12601 caatgaagaa aatactatta taggtttttt aaataactat aaattaaagg aagataagac
    12661 tagttattat gtgtgtcaaa gtaatagttg tagccaacca attaataatt tacaaaaatt
    12721 aaaagatatg attttaggag tagaataacc agaatagtga aaaaggagat gtcttaaagt
    12781 aaaaatctaa tcatctcctt tttttgtaca aaaaacaaaa aatagtttct aagaacactt
    12841 aatctatgta ataattgttt tgttaaaaga tgaaaatgat attatagtta tataaaatat
    12901 tgcattataa aaaagtggta ttattataga tgttatagta ttaatcgttt ttatttacct
    12961 atttcttatt tttacaaatt cttcaagtgc ttcagattta attttattta tttcttcata
    13021 ttcccaaaat tagcaccttc tttaaagccg tcttgttctt cattattaaa acaatacatt
    13081 ttttagtaaa ttccacaagg tttccaattc atctttgata aattcatgaa atacatctgc
    13141 cattaagttt attacagctc taccacatgt taccataact tcttttccta agtctataac
    13201 tttaacagta attgctctct tatcatgagg acttgtaatt acatcaatat atccattttt
    13261 tttaagatta acaactatcc tatttatatt ttgtttactt gtgaccattt ttcttgcaat
    13321 attatttaat gttgtttctt cttctgataa atgaagagta gataaaatag tcatatactg
    13381 tcttgaagta agtataccaa aatatttata tccttctatt tgtattttgt ttgtaatggt
    13441 aaaaaagttg catatgtttg attcattaaa aagagttctt ttagttaatt tgaataatct
    13501 attaaatatc tactttatct ttagatgttg ttttgtcgaa aataaaatat catgaattaa
    13561 ttctaatgtc aatatggtat caaaatatat taaagtatta cttttttatg caaatattaa
    13621 ttaaattatt atataatttt atgtaataat ttctatacac aatgataact tctatgaagc
    13681 attaataatt cttaaatttt tttagaaaga ttttgacttg aaaacacttg taatataata
    13741 tataaattaa tatactgaat aaaatcaatc tggagggata attatgaagt gtacaaactt
    13801 tactttcaaa ggagaggaag gtttagatat atatacatat aaatgggaag atgaaaatat
    13861 aaaaaatcca aaggctgttg tccaaattgc gcatggaatg gctgaaactg cacaaaggta
    13921 tgagactttt gccaaggtgc ttactaaaaa tggttatata gtatatatca atgaccatag
    13981 aggtcatgga aaaactgcaa agataataga aaatgttggg catttagctg agaaagaagg
    14041 ttttagatgt cttgtagagg acatgtatac tttaacaaat attataaaga aagaaaatga
    14101 agatttgcca atttatctat ttggtcatag catgggttca tttgcaagtc aaagatatat
    14161 aatggattat agcaataatt tatctggtct tatactatgt ggttcaaatg gaaggcaagg
    14221 aatcattcta aatcttgctc atatgataat caatcgtgag attaaaaagt atggaagacg
    14281 ttctagaagt aataaaataa atgatttgat ttttggtggc gaaataataa gaagaaatga
    14341 gaagactaag tttgattggt taagtagaga taaagaacaa gttgagaaat atatcaatga
    14401 cccattttgt ggagtggtgt gtagttgtgg ctttttttat gatttaattc aagggttaaa
    14461 agaaattgag gataaagaaa acttaaaaaa agtgccactt gatgtaccta tttacataat
    14521 atctggagat aaagacccta tagggaaaaa tagtaagggc gtcttaagac taagggatag
    14581 atatataaag ttaggagtaa aagatgtaac ttgcaaactt tacaaaggtg gaagacatga
    14641 attattaaat gagataaata aagaagaagt ttttgaggat gtaatttgtt ggctaaacaa
    14701 taaaatagga atattgtgat aaaggaatat tatattaata ttaaatttaa ttctatgtaa
    14761 atgtttgaaa agtatattta atatgtgtaa aacagaaaag aaagtgtttc aagatgatta
    14821 tttattatca atgaaacacc tttttatttt ttgataaata tatttaccac tttcaagaga
    14881 agagtgtttc aaaatcttaa gaaaatcttt tgatttaagt atatgttttc ttaagaaaat
    14941 taaattattc tattaaaaga actagaaata agtaagtgat tttggttttg ataaatataa
    15001 gaatctttat ttattaagtg cgtaaaaaat ataaatttat ttggttttaa ttccaaagta
    15061 aaacttaatg gaggtaatag atatgagcaa aatattaaga gtaaaaaata taaaaaaata
    15121 ctataaaagt agaggtaaca ttacaaaagc tattgataat attagctttg aagttgaaag
    15181 tggagagtat ataggtatta tgggagcttc aggaagtgga aaaactactt tactgaattg
    15241 tatttctact atagataaaa cgacagatgg gaatatatat ataggaaata aagatataac
    15301 aactatgaat gaagatgagt tatctaaatt tcgtagagaa gaactaggtt ttatatttca
    15361 agattttaat ttattggaca cacttacagc acatgataat atagcattgt ctttatctat
    15421 caatggagtc aattcaaaag aaatagataa taaaataaaa aaaatagcaa ataaattagg
    15481 tgtttatgaa atacttaata agtatccata tgaaatgtct ggtggtcaaa agcaaagaat
    15541 agcatgtgca agagctataa taacaaatcc atcactaata ctagcagatg aacctacagg
    15601 agctctagac tctaagtcag caaagttatt tttggaaaac cttgaagttt taaatcgtga
    15661 attaaaagct accatattaa tggttacaca tgatgctttt acagctagtt attgtaaaag
    15721 aatattattt ataaaagatg gtaaaatatt caatgagttg gttagaggga ataatactcg
    15781 tcaagaattt tttgatagaa taataaaagt aatatcagtt cttgggggtg atgataaaca
    15841 tgttttttaa aaaaaataaa tcaagaaggt ctagaaataa aagaaaaaat aattatgttt
    15901 caagagtgtc aagtgaatca tttaaaaaat tagctttaaa taatgtgaaa aagagtctaa
    15961 aaaattattt gatttatttt ataacccttg tatttggtgt tattatttta tacactttta
    16021 attctttaga caatgatata gcaattctta gtagtaatgc tttattgagt tcaagtatat
    16081 cgtttgttag gggtattgta agtatttttt caattctagt ttgtattata tttgcatttt
    16141 tgattattta tgcaaataac tatttaatga aacaacgtaa aaaagagttt ggaatataca
    16201 gtattcttgg aatggataga agagatataa ataaattaat gtttagagaa atgatgataa
    16261 taggcatatt ttcattaata ataggtatta tatttggagt atttgtttct aagggactta
    16321 gtatatttgc cttaaagatg ttgggcatat catcatcagg attttctttc tcaatgctta
    16381 caactattaa aacaatagta ttttttggca ttgtactgtt attagtcaat aaattcaata
    16441 aaaaaacaat aaaaaaatac aagttaatag acttattaaa tgcaaataaa aagaatgaag
    16501 tttcagttgc aaaaggaaaa aaatctaatt tgatattatt ctttttatct atagtggcaa
    16561 ttgttttagc atatataata ccattgaaaa tgaaaatatc tacatttata ttaggtatat
    16621 gtttaatttt aatattagtt ggaatttatt tgttttttat gtcagtatct gattttataa
    16681 tgttaaaaat aaagaagcat agatttatat actataaaaa tttaaatcca tttatagtaa
    16741 gccaaataag tagtcaaatc aaaacaatga gtatgtctat tactatgatt tgtatgttat
    16801 tattttcttc tttgatagta ataccatttg gtatatcatt tggaaaatat ttaatagatg
    16861 atttacaaga agcgacgcct tatgatgtaa cattatctaa attgtatatg aatggagata
    16921 acattgtaga atacagtaca aagaaaaatg atatagatag taaaactcct tcacttaaag
    16981 atgaactaat agaaaatggt tttccactaa cttcattttc taaaagtaca agtgatttaa
    17041 ggatatataa tttaaagaac ataagtcagt acgattttat atcaaatgat aaggatagga
    17101 caaatgaact gaaagataat aaacttcata ttgtctcaat tagtgattat aattttacta
    17161 gaaagcaaag tggtttaaaa gaaataaatc taaaggataa tgagtttgca ataaattgtg
    17221 cctcagaaga ttatagacca ttttatgaaa agtacattaa gagtcagcca cagaattata
    17281 taacagtaaa tggagtaaaa cttaaattaa atcagacaag cttatatacc aattcttata
    17341 atactaatac agttccttta gatttgggaa ctataattgt tccagatgct gtagtaaagg
    17401 atttaaatcc tattatgact acattaaata ttgattacaa agaaagaagt agtaatcttg
    17461 aaaataaatt tatggattca tactataaat ttgaagagaa aatcaataat tctgaagctt
    17521 atagtatttt atataaatca gttatagatg gcgaaaaaag agccttaaac acttcatttt
    17581 cttttgtagc aatatatata ggtattatat tattaatatc agcaggtgca atattagcat
    17641 tacaacaact catagaatca actgttaata aagaaagatt taaattatta agtaagcttg
    17701 gagtaaataa gaaagatatg aaaaaagcta tttttataca ggtatcaata ttatttgcag
    17761 tacctttaat attggctctt ataaatgctc tgtttatatc aaaagtattc ttcgttataa
    17821 tgccatttat tgcagagaca ggtattatta caactatgtt attaacatta ggtattgtta
    17881 tcgttgtata tggaatttat tttatttcaa gtttacttga aagtctaaat atagtaaatg
    17941 atagtaaaga gataatctaa gtctttaaaa agactattta ttgtgaatct attaattaag
    18001 ttacacttaa gttagcttag ttaatagatt ttatatttta attcaatgaa agaatctttt
    18061 agactaaaat ataaccaata gttaaattat gaatatttta ttaactttga ctcaatttac
    18121 ttgtaagtac tttttttata tacttaaatt acaatagtta tgctagggag gtatatatgc
    18181 aaattaaaat aggtaaaatc atacaagctc ttagaaaaga aagaaatcta actcaagagc
    18241 aattggcaaa atttatagga gtatcaacac cagcagtatc caaatgggag agtggaaatt
    18301 cttatccaga tatagaatta ttgccattat tggcagattt ttttaatgta tctattgata
    18361 agcttttaaa ctacaagatt gatttaagtg aagaagaagt gacgaaaatt tataaagaat
    18421 tagaatcaat ttttgcggga attgaagttg atttatcaac agaggaacca aaagaagaat
    18481 gtaagcgaga cttggaatat gtaaaaaacc tttctaatat gtatatagaa aagtatccta
    18541 aaagttatct attaaagtta aaaatatgtt cgttgtatca gatgtattca tataaatttg
    18601 gcaagaatga ttttaataat aacgtaagag aaacaacaaa tatacttgaa gatatagtaa
    18661 gaaatacaga tgatattcaa ataaaagaaa cagcactcat tatcttatca aatgcatatt
    18721 gtatgttgga ggattatgaa aaggcagagc tgtacttaaa tatgatacat aaaccaatag
    18781 gagatacaag tgtcaattta gctatgatat atttaaatca aaataagcta gaagaagcag
    18841 aaatattgct tcaaaataaa ctgtttagta atgtattcaa tttaagtttg gattgtaaag
    18901 gaataatcaa tgtatataaa tatcaatata aggaattaaa gaaaaaattg gagtatggag
    18961 attctaataa aaaagcaata gagaaagaaa tggaatatat aaaaaataag ttgctagggt
    19021 atgcaaacct ctctttagaa attaaaaaaa tgattagtga agataaaggt gcattttttg
    19081 gtatatatat ggattatatg gaattatctt taactttttt atttttcaat atgaaagaag
    19141 aagccaaaaa agctttatat agtctaaaag aaatattaga aaaataccct atacatgaaa
    19201 gtttggatgt tagtcagatg agattttttg acaaagtaaa atctaaaaat tcatatacct
    19261 tcaatatgta tacgaattta ttaataatgt ttagtgatga tagctataat gaacttagag
    19321 gagaaccaat tttcgagtct ataattgaaa agattttgga gttggaaaaa ttattaaaag
    19381 ataaataata aataaaaatc taatataaga tttaaagatt aattaaatta aaaggagatg
    19441 tgtcaaaaca caaatttgaa ccatctcctt tattacataa aaaaaataga aaagggcttg
    19501 ttaagaagta ttctaaaacc attaaatcaa atgattatat caaaagaata gaaatatatt
    19561 aaaaataaac aaatgaaaat taagaagctg atatattttc atcattttaa caaaaaagag
    19621 acctatctct attttgagac agcctctttt atgtaattgt tatgaaactt tgcacaactc
    19681 aatagatagg aactatactt agaagttcat tacatgattc tttaaatact gattaaattt
    19741 atcatattcc attttagata atctttctgg ataagacatt acaactagtt gatgaccatt
    19801 aaatttctct tggtatggtg gatgaatatg tttataataa tttgtcttat attttactct
    19861 ctcatagaat ttcttgcgac gagttgaaag ctcatcaatt ggagggtcaa tttctaaaat
    19921 aatagttttt cctctcttat ttagcagttc taacgttttt tgtccatatc tattattacg
    19981 catttctgga aaaatacaga aatgttctac ataaataaag ctttttgttt cccaacatag
    20041 aattatgcca acaaactgat ttttatcata aattaaatta aattgatatt ctttattttc
    20101 cataattttt gcttgcaaag aagattttct ttgttcatga aatggaaaac tagttttata
    20161 taattccata gctttatagt ataatttatc tttgctagtt cttaaccgct caaatttcat
    20221 tttgttcctc ctaattacga aaaattccga tttatatatg aatttatttt atcacagtca
    20281 gcatttgtat acaatgtgtt ttcagttgta tattatttct caattttatt ccacttatgt
    20341 ctacatctca tgggctgaaa tataattgtt tattgtgtaa cctcaaacta ttatacaggt
    20401 tctgatggct taaaaacagc atacttatag aattttttta tgtatgaaaa tgattttaat
    20461 agttttagaa gttacaaaaa agttacataa atttggaaag ctataatata catatataat
    20521 ataaggttaa aactgtaata aataatgtta atttaaaatt tgaacatgga aggagattat
    20581 tatggagaaa agagaaaaga ggataataat atcatcttta ttaagtgtat caattttaat
    20641 gagtttggta agtatatatt ctatattaaa taaagaagac ataagtttaa ctgttaaagg
    20701 tcaagagcaa aaagtatctt cctttaagaa aacagttgaa gaacttttgg atgaacaggg
    20761 tgtaaagtat aattctgaag ataagattaa tccaagttta gatacagagc taaaagatga
    20821 tatgaaaata aaagttgtta aggtaactaa aagtaaaaaa gaagaaattg aaaaaattcc
    20881 gtttgataca aagcatgtaa atgatagtaa tttgttaaaa ggaaaatcta aagtttatca
    20941 agagggtaaa gagggagaaa aaaaactaat ctataattta acttaccatg atgaaaagtt
    21001 agtcaaaaaa gtcttatcaa aagaagtaat atctaaggag ccaactacaa aaattataaa
    21061 atatggaact aaagaaaaag tactaatagc atcaagagga gcaaatataa gaggaagtaa
    21121 acatatgaaa gtggttgcta ctgcatatgc aggagataca ataacatcta ctggtacaac
    21181 tccaagatgg ggtgttattg cagttgatcc acgtgtaata ccatatggaa caaaagtata
    21241 tatacctaag ttaggtatga cttttgtggc agaagattgt ggaggtgcaa ttaaaggtaa
    21301 tagaatagat atttttatga atagtgaagg aaaagcctca aattggggaa ggaaaagcat
    21361 agatatatat ttacattaaa ttagtgaata gaataacttt acttgaaaga taaaaaagta
    21421 taaaatatga attaactaaa ctattagatt attcttaaaa cataaaaata tatctaaggg
    21481 gggataagat gaaaatagtc tgtttaggag acagtttaac atatggattt ggagtatcac
    21541 gaagtaattc atggacaaac attgtaaata aagagactcg attagaaata gtaaataagg
    21601 gtataaatgg tgatacaaca agtggtatgt tagttaggtt caatgaagat gttgtaaaaa
    21661 actctcctga tatagttttt ataatgggtg gtaccaatga ttttattgcg ggtgcaggaa
    21721 atgaagttat taattctaat ataatggcta tggtacatca gtcatattca aaaaatatta
    21781 tacctataat aggtatacca ttaaaaccag atattccaaa tattagggaa gattggagtt
    21841 gttttactga tttcaatata gtatctcaaa agttagaatc atatagctat tggataaaaa
    21901 aattctgtat gacatttaat actaatttcg tagattttta ttcagaatat aataaaaata
    21961 tggaactaga aggatataaa aaattatatt ttgatgggtt acatccaact aaggaaggtc
    22021 atagaataat ggcagacatt tttattaatt caattaataa gtatatagaa gagttatagt
    22081 tacatcaaaa tacttgttta tgtgtcagtt tcaataaata catacagtag agaggggttt
    22141 attatggatt atactaaaac ttatgaagag tggataaagg gttcatattt tgatgaagat
    22201 acaaagatag agttggagaa aataaaaaat aatgaaaaag agatagagga tagattctat
    22261 aaggatttag aatttggaac tgcgggtctt agaggaataa ttgaagcagg aacgaataga
    22321 ataaataagt acacagttag aagagctact tttggattgg caaactatat attagaaaac
    22381 acaacaaaag aagaaacaaa tagaggagta gtaatagctc atgacaatag acacaagtct
    22441 agacaattct gtatagaatc tgcaaatact ttggcagcat gtggtataaa agcatatata
    22501 tttgatagtt tgagaactac tccagaatta tcttttgctg ttagaagctt aaatgcaatc
    22561 gctggtatag tcataacagc aagtcataat cctccagaat ataatggata taaggtatac
    22621 tgggaagatg gagcacaagt tatgccagag attgcaaatg caataacaga gaaagtaaat
    22681 agtatacatg attatagtat aataccaacc ccaagtgaag ctaataaaaa tttagtagtt
    22741 ttattggatg aatcacaaga tactaaattt atagaagcag taaaaagtca aattataaga
    22801 aaagatttag ttaaaaatgt aggtaaaagc ttcaagatag tttatacacc tctttgtgga
    22861 accggaaata ttccaataag aagagcatta aaagaagtgg gttttgaaaa tataattgta
    22921 gttccagagg aagaaaatcc agacccaaat tttgcagggc ttgattatcc aaatccagaa
    22981 gagaaaaaag ctttaaatag aggtatatta ctcgcaaaag aaaaaggagc agaccttgta
    23041 atagcaactg acccagattg tgatagagta ggtgtagcag taaaaactac tataggtgag
    23101 tatgctttac ttacaggaaa tcaaattggt ggtatgctaa cacattatat aatagaagga
    23161 ttaaaagaaa ataataaatt aaaagaaaat cctacaataa taaaaaccat agttacttca
    23221 gaatttggag cagatatagc taaagcaaat aatgttgatg tattaaatgt tcttacaggt
    23281 tttaaattta ttggtgaaaa aataaaatta tttgaacaaa ataaaaatag aagttatgta
    23341 tttggatatg aagaaagtta tggttactta gttggtactc atgcgagaga taaagatggt
    23401 gtagtttctt cgttattaat atctgaaatg gcagcctttt actattcaaa aggtatgagt
    23461 ttatatgaag gtttaataga actttatgaa aaatatggat tttttaaaga acagaccata
    23521 tccttgactt taaaaggtat agaaggtgta gaaaagataa aagaaataat atcttacttt
    23581 agggaaaatc aaattgatta tataaatagt gttaaagtca ttgataaaaa agattataaa
    23641 aatggtatag ataatcttcc taaatctgat gttttaaaat actttttgga agatgaatct
    23701 tgggtagcta taagaccatc tggaacagaa ccaaaactta aattttacat agctgtaaaa
    23761 ggagctagtg atattgaagc agataaaaag attcaaggtt taaaaaaata tatagatgac
    23821 atggtcggaa aattaaaata aaatattatt attttataat aatatttatt tagctgaaaa
    23881 ttataattta ctttcattga ttagaatatg taaatgttag tagaattgtt attttcagct
    23941 tttatttgtg ttatttttac ttaaataatt atattatagt atgaatctga gtataattaa
    24001 tattgtataa tgaaattaat ttgatagaaa aggggatatt tatgtttggt atagataaga
    24061 aattgattga atatataatt gcaaaatcca attacagtga actaagttca aagtcagcag
    24121 aagtatcttt cttcttaatg ttgtccatat ttcctttttt gatatttaca ataagttcta
    24181 tcgcttatat accaattctt catttaaata agtatatagc tttgtttaga aatatgatgc
    24241 cagaaggagc ttttgccgta ttatcttcaa tcatagtttc tgcaatagac aatagaaatc
    24301 tgaaattttt agcggtcagt tttgttctta ctatgtggac attttcaaga gcagtaaaag
    24361 cccttattaa aggaatgaat agagcttata aagttaaaga aactaggtct ttttttaaaa
    24421 ttttatctat atcattttta tttacaataa tgcttttggt tcttattttt ttatctatga
    24481 tatttctagt ttatggggaa aaaataggtt attttatttt taatctcata ggactagatg
    24541 agatatttat aaaaatatgg gatattctaa gatacactgt aggtataata acaataatag
    24601 tgatatttac acttttatat aaatacactc caaataaaaa gctaactatt aaagaatctg
    24661 caccaggtgc tatttttgct acttttgctt ggtttttagt atcattttta tattcttatt
    24721 atacaaatta ctatgctaac tatgaagtaa tctacggaag tatagctgaa ataattgtac
    24781 ttatgacctg gatgtatttt agtagttggt ctatcgttat tggttatgaa gtaaattcaa
    24841 gattgtattt tagaaagata aaacatgaaa tgttaaagta aagcattaaa ataaaggata
    24901 ttatttaata ttataaatgt aattgaatta ataaaaaata ttgacattta agttaaaaag
    24961 tctagaattt ttctgggctt tttttatttt tagtacaact tgacaaactt tatttaatgg
    25021 gatagtttta atttataatc atctttgaaa agatgactat tgataagcaa gagatgaaat
    25081 tggtaaaagt ataaaaaaat atactaaata aagattatag tcctaatgag ttaatgtctg
    25141 tgttttcaaa taatgcagta tttagtcatg tgtttttata tgcgcctgta ttcctaatta
    25201 ccaatatata ttagaataag ctactgtaat tagatttaga aaaaagcgac ataaataatt
    25261 ataaacaaat aataaattac ataaaattaa atttattcac aagtatatct tataaaatat
    25321 gttataatta atagaaaatt gtcatataat tacttaagaa gtattattat aaataacatt
    25381 gagaggtagt ggttagaata tggataccca taataaatat gtaaatttta taaaaaatat
    25441 acctgtacct tttttatatt gtagaattgt aaagagacaa gaagatatag aatatcaagt
    25501 agagtatata agtaagggta tgggcaaagt attacaatta gaagaaggta tttgtgataa
    25561 aaatatatta gatgtattgc ctgtatttaa atctaaaaaa tatttcaaag agttgttctc
    25621 aaatgaagtg gattgtataa aaaggtacat tccaacactt aaaaactgga taaatataaa
    25681 aaaacagata gtaggggatt catacataat tttgtatttt ggtaaaattg tatttgatta
    25741 tagacagata atcgattctt ttgataaaaa ggaaaaagta gcttatatta aagatgaaga
    25801 aggcatatat atagattgca gtgaaaattt aataccaata ttaaataata acattaaaac
    25861 aacaaaagat atctttggta aaaatgatat agaagtatgg ggagagagta caggaaaatt
    25921 atttaaagat gattatagag aaggcgtatc tagcaaaaaa agatttttac aaaatctttt
    25981 tgaatatgaa gaaacatttt tcatggttga aaaatatttt ttgtatgacg aagatgaact
    26041 tttaggtact atagggataa tagacaatat aatatatagt ggttacagta ataaaaatta
    26101 taactcaaaa gacttaatga agatgataga gcactcaatt ccagagaata tgttttataa
    26161 agatgtgtat ggaaattata ttggatttaa ttcaggtttt ttaaatttag cttgtatgaa
    26221 taaagaagaa ttattaggaa agaatagtta taaaatatca gaagaagaag ctttaataga
    26281 taagatattt gaaagtgata agggtgtagt tgaaagtaaa aaagtagtta catttgagtt
    26341 aaatataagt atgaatgatg aaaacaagtg tattgagatt acaaaaaggc cattttttga
    26401 tagctatgga agtgttattg gaataattgg aacagcaaag gatataagta gaagaaaaag
    26461 attagaagag gaaatggata aaactaggat ggaatttttt gcaaatttat cccatgaact
    26521 tagaacacct ataaatttaa tatcatcttc attacaagtg atagaaaaaa aagaagcaga
    26581 cttaatagaa tcaaatgata ctttaaaaag aaatctaggc ataataaaac aaaatggaaa
    26641 tagaatactt aggttagtaa ataatgttat agactttaca aaaattcagt caggatattt
    26701 agattttaaa ccagaagaat ctgatataat tgcatttata gaagagatat gtatgtctgt
    26761 agccgacttt gcaagtcaaa ataatataca acttacattt gatacagaaa tagaagaatt
    26821 ttcaatgcta tttgattcag aaaaattgga aagaataata ttaaatttac tttctaacgg
    26881 aattaagtat aataaggaag atggaaaaat aaacatattt ttatatgtga aggataatgt
    26941 atttaatatg aaaatatctg atagtggtat aggtatacca aaagaaaaaa tagataaaat
    27001 atttaatagg tttgaacaga ttgacaatga actttcctac agagttaaag gaagtggtat
    27061 tggactttca ttagtgaaat ctttggttga gttgcatgag ggaagtatat ctttaaaaag
    27121 tcaacttggt gttggaagtg aatttatagt ttcattacct gttagaagta aaaataatat
    27181 tgaaaaatac aatcataaaa gagaaatcag taatgaacta agtaaaaaat tagaaataga
    27241 attttcggat ttataagttt aagtataaaa aataaaattt taaaaaagta ttgacaattg
    27301 atttagaagg gtttaaaata aattacaaca taataaataa aaaagcgatg aataggaaga
    27361 gtaaatctaa ttcgattgcc agagagaaga gtccattggc tggaagactt tttaaattag
    27421 agggattgaa aactacctag gagctacttt ctgaagttat ctacatcagt agattttagt
    27481 aagaatagtc gttaactgcg ttaaagtgta aagtgagttt tcatgatata tgaaaactaa
    27541 attaggtgga accgcgggaa taatactctc gtccttatgt tataaggatg agagtttttt
    27601 gttatttaaa aatataaaca gtaatcataa taattataga aaatataaaa gcgatgaata
    27661 ggaagagtaa atttaattcg actatcagag aggaaagttc gcaggctgaa agactttcta
    27721 agtttgagaa attgaaaact acctaggagc tgtggtctga agttacttta aaaagtttta
    27781 gtaagactaa ccgttaactg cgttaaagtg taaagtggat tttatcaatt aatgataaaa
    27841 tcaaattggg tggaaccgcg agtatagact cgtcccttta tatagggatg agtctatttt
    27901 tagtgcaata aaagagtgaa aataaaaaaa aatcggaaaa ttataaattt aggaggaaaa
    27961 taaaatggag aataaagtga aaattggagt actaggatat ggagttgtag gaagtggact
    28021 tatagatata atagataaca ataaagaaaa aagaaacata gaaatagttg ggattctagt
    28081 taataatttg gaaaaacata agggtaaaaa atattctaat atgataacta ataatataga
    28141 tgatatattt aataaagata tagatatact tgttgaagtt atgggtggtt tagaaccagc
    28201 attatcttat ataaaaaaag cattaaacaa taaaattcat gttgttacag caaataaaga
    28261 tttgttggct gaatgtggag atgagcttgc aaaattagca agtgaaaata aagtaagcat
    28321 taaatttgaa gcttcagttg caggaggtat accagtatta aaaccaataa tagaatcact
    28381 agaaggaaat aatatagata gtataaatgc aattttaaat ggaactacta atttcatatt
    28441 atctaaaatg tatgatgaga atctatctta tgatatggcg ttaagacaag ctcaagaatt
    28501 aggttttgct gaagcaaatc cagagtcaga tgtattaggg tatgatgctg ctagaaaact
    28561 atcaatatta tcaactctag cttatgacaa tagagtatat tggaaagatg tgtatttaga
    28621 aggaattaca gatatagatg aaaaagatat tgaatacgca aaaaaattaa attgtaaaat
    28681 aaaattgatt ggtcaaagta agtatgaaaa tgataaagta agtgcttttg ttagacctgt
    28741 tttagtagaa aaagataata tacttgctag aatagacaat gagttcaatg ctgtcatagt
    28801 gaatggagat tcagtaggag aagtttcttt tgtaggtaaa ggagctggaa gtttagcaac
    28861 aggaagtgca gtttactcag atgttataga tataatagat aatagagttt caagtataga
    28921 ttcttttact aaagataaaa tacaagttaa taaaatagtt agagaaaagt gtggagcact
    28981 tcttagattt aaaaaatgta ataaagatga aattttaaat atagttgaga attgtttagt
    29041 taaatttgat attttaaatg atgatgatga acttgctatt atggtttatg ctgattctga
    29101 atatgaaata aataacagtt tatgtctaat aaaagataaa ggatattgcg aaaaaatgaa
    29161 taaaatgttg aaaataagtt aaaaaagaaa aaatataagt ttatatgtaa catattttga
    29221 cgattttaat actatatagt atgagatgaa acaaaacatc tcataaaaaa attaaaatta
    29281 aaggggagat atgttatatg gaaaataaaa aatatgcaaa tggtggttat tcagaagatt
    29341 ggtatgaaag aggagaatct acagctaagt ggttccaaaa tgatagagaa gaatatgaaa
    29401 gagcagcata cgatgaagat agagaaagaa gaggttcaaa ctgtggaggt tcagattcag
    29461 aaggaaatag acctagaaac tgtgaaagat ttagaagaga agctgagata agagaaagag
    29521 aagcaagaga agcattttgt gaatcttcag agagaaagaa agaagcatta gcatatgaat
    29581 gtgaagctag aaaattatgg gaacaagcag aaaaatactg ggatgaatat tcaaaataca
    29641 actatcaagg aatcgaatat ttagcagaag ctgctagatt atttgatgaa ggtatggaat
    29701 gtgaagctag aagaaatgga aataatggag gaaacaataa taattgttgc cacaaatgcc
    29761 ataaatgtaa ttgtaactgc tgtagaaaat aaaaaaattc attgacaaat caatagttaa
    29821 aatatatcat attatgtata ataaagagaa aattcagaaa aaaataatga ttataagaaa
    29881 tataagtata tttattagac aaatagtgct cagttagtga ggtgagtaca atatgattaa
    29941 actcccatcc tctagctctt gatagagggt gggagttttt attattgata tattaaatga
    30001 ggataatatt gtaaaattag gaggtaaaaa atgaaacgta ttaaaacatc aagtatgaaa
    30061 gaaaaggtag atgaaaagat attaagagag caagtaagtt ctataataga agatataaga
    30121 aataataaag atattgcact taaaaaatac aatgagaagt ttgatagaaa tacaagagat
    30181 gaatttagaa ttactaaaga agaaataaag gaagcttata agcatgtaga tgacgaattt
    30241 ataaataact taaaaattgc tgccaaaaat ataaaagaat ttgctaaagc tcaaaaatca
    30301 agttttgaaa attcttttga aaaagaaatt tatccaggtg taattctagg tcaaacaaat
    30361 attccaatag agtcatgtct tgcatatgtt ccaggaggtc agtatccatt gttttcaaca
    30421 gctcttatgc ttataatacc tgcaaaagta gcaggagtaa aaagaatagt ggcatgttca
    30481 ccaactatga aaaatactga aaagataaat cctaaaacct tggtagctat ggatatagca
    30541 ggtgctgatg agatatatgc aactggagga gttcaagcta tagcagcatt tacatatgga
    30601 acagaaaaga ttaaaccggt tgacataatt gtaggcccag gaaataagtt tgtaactgaa
    30661 gctaaaagac aatgttatgg acaagtagga attgactttg tcgctggacc aagtgaagtt
    30721 cttataatag ctgatgaaac ttcaaagcca gtttatattg cagcagattt attagcacaa
    30781 tgtgagcatg atttaaatgc aagaggtata ttattgacaa atagcttaga aatagcagaa
    30841 gaagttgaaa aaaatataga aactatgcta aaagatttgc caacaaaaga tatagcatat
    30901 agttcatgga aaaataatgg agagataatt ctggttgatg atatggaaga agccattaaa
    30961 ataagtaatt tttatgcacc agaacattta gaaattgcag taaataagtg tgatgatatt
    31021 tttgacagat taactaacta tggttcttta tttattggaa atctttctgc tgaggtattt
    31081 ggtgactatg tttcaggtac caatcataca ttaccaacgc ttaaagcatc aagatatact
    31141 ggtggtgttt gggttggaac ttttatcaaa acttgcacaa agcagatatt taatgaagaa
    31201 gctatagaat cattagcacc tgtagctgaa aagttggcaa aagaagaggg attatatgca
    31261 catgcaaagg cagctgaggt tagatttaaa aagtagtaaa aaattctatt ttcgtcagtc
    31321 gagatttagt attagactga gataaggaaa acgccgagaa catgaataaa tactatattt
    31381 tgtgggtaat cggcgtttct tttatatctg tatatgatag gataatacta cctgtgtgtt
    31441 taaatgataa cttgatattt cttttctaaa taatgtttat gaaatagtta atatttttag
    31501 ttaatacttg aaaaaaactt ttgctaactg tgcaaattca tctgcatttg tcaatatagc
    31561 tgggtgtcct ccatgactaa aaatgtgaat acttgctttt cgaataaaag ttgccatttg
    31621 tttgtactct tcttcaagat tgtgacgaca catttcgtct tccttacttc ctataaacaa
    31681 aattggcatt tctaatttac acaattcttt atgaaacaaa ggacgtttct cctttgcaca
    31741 ttgcaataga gcttttgtat caagattaac aacattttcc caatcttttc cctgacacca
    31801 ttcatagaat tttcgtgctt gaatatcttg ctttgatttt tcccttccag tgattagatt
    31861 atctatgaag ttatcattaa gagtacgccc atcaaagcta tctgctataa ttgcttcaac
    31921 taaatcggga cgttctaatc cagtattaac agctgcccat gctcctccac ttgttccaat
    31981 taagccaact ttaccacaat tcaaatgttc aatcagtgct atcgtctgta aggcttcatc
    32041 atgccacatg tcaggagaaa attgagaaac tctatccgac tgtccattac ctaaaaaatc
    32101 aattaagata catctaaagt tttctacata taatggcata agtgctttaa acatattaga
    32161 agaagcagta ttaccatgca ggaaaactat cggtttacct tgtccatatt cttcataaaa
    32221 acaagcatta ccttgatact gaaaatatga catatattgt cctccttttt atttgttaag
    32281 ttaattataa caatataggt atgaatgaaa aacctcatgt tctttcttag aataaaaatg
    32341 tgccacgcgt aaatcgtgac acatctctaa ttataaattt tgctgtttaa gtattctttg
    32401 tatgcttttc tctgtcaaaa agtatttctc tgataactgc ttgatgctca ttcatttttg
    32461 gaagtttata cagatttgag aattacgaga agctaagact tgtttggtgt ttgtgttttc
    32521 cccccaatgc tttttgtgct cttgcttttt gggaatataa atcactttac catctatata
    32581 ttcttgcact tgctcaatca gttctttagg cagtaagtca atcgaatttt gatagctcat
    32641 tttgcacctc ctaaataatt gctctttagg attagcaaag gctactgcta ctcataatta
    32701 aaaataaaat tttgttatta tgtacaatag cctttgctat gcacaagttc caattcccat
    32761 aaactcaata ctcctttctg atttaaaaaa ttcatctgaa cattgatttc gtttttaaaa
    32821 tagagttcaa tgacaaattt acatttctat tatatatatt caggtagcta attgcaactg
    32881 tctttgtatg agggaatttt attatagtca atactataat atgtatttat atcatcaaat
    32941 taatatgaac agttgaaagg aacatgaata ataaaactta gcaggaagaa taattataaa
    33001 gcagacaata gagaaatttg attctaaggt ttttttgaaa attcattaac aaatttaact
    33061 tgtaaatatt atactaaact taataaataa agtttataaa gtaaggagtt gtctatgaaa
    33121 aatatagttg gaaaatcaca aacgataaaa aaagtaaatg aagacttaat aaaaaatatt
    33181 ataaaaaatg aaggtcctat aacaaaacta gaaatttcaa gcattactga attgagttta
    33241 gctacagtta ataagattgt agagcagtta gctttaaaaa atgaggttaa agtaagtgga
    33301 ctaagtgaat ctactggagg aagaagagct caattatttg aaataaatgc aaacttagaa
    33361 cacataatag ctttatacta ttatagaaat tattgtatag gggtagttgc caatttatta
    33421 ggtgaaataa tttatcaaga aaaatttaat ataagaatgg atacgtatta aaacgctaac
    33481 caaaatactt tttttgttat agatacttta ataaaaaaaa gaaaaaggaa ataatataag
    33541 agccattgga attggcgtcc caggagttgt aaaaaaaggt atagttaaaa atataccaac
    33601 tataaaaagt tgggaaggta taaatttaga aaatttaata aagagtaaat acaatactaa
    33661 attatttata gaaaatgata caaatattac taccattggg atttatcaaa aacagtataa
    33721 agaaaaatat aaaaatatgg ctcttatgta tctagaaaat ggtattggtt caggtatagt
    33781 tataaatgac gaattatatt taggttctac aaattttgct ggagaattaa attatctaaa
    33841 tatagaattt ggtaagtata actctaataa cttaaatctt gaagagtata tgtatctggt
    33901 atatctggtt gtgattctcc taatgactat aagattataa tggatgaaat attaaaaatt
    33961 gggattaaca aagaaaaaat atatttagaa aatgatgcag ttttagcttt ttatgcacaa
    34021 gctgattcac atggtttagt aattgtggct gggacaggtt caattatatt aggaattaaa
    34081 gaagatggag gataatattt cagagttcaa gggtgaatgg taaattgtca tagataataa
    34141 ataaaatttt cagagttact ttatatagtt tctaaagtaa ctctttttta aattgaattt
    34201 agtgataaat tattgaatag tatttctaaa caaataaaaa attaatataa acaaagactt
    34261 aattatacta aattataagt caaactaata taaacttata taaaaaagga aaacgtctaa
    34321 agtattggaa ttactgtgat ttaggtatgg tattaaaaaa acataaaaaa acataaaaaa
    34381 attgttgaaa aaactataaa aattttgtat aattaaagat ttgaaaagtg cggtatacaa
    34441 agccaattct tttgtatata atatatataa attcagaaaa aggaagggat atgtatggcc
    34501 aggcggttat ttttagaaag gattggactt atatttttag gttcagcaat attagcattt
    34561 ggagtttata atttttatta tttaaacaac ataacagaag gtggagtgtt aggtatttta
    34621 ctactgttaa aaaatttatt caatatacaa ccagcaatag caaatgttgt aatagatggt
    34681 ttactacttt tagtaggata taaatttttc ggtaaaaaat tccttatata ttcaatagta
    34741 gcttctataa ctttttcagt gttatatgac ttatttgaag ctataggacc tctagtacca
    34801 caatcacaaa atatgctttt atcaactatt ttggcaggtg taactgttgg aactggtgta
    34861 ggtatagtag taaaagcagg ttgtgcatca ggaggagacg atgctttagc attagtaatt
    34921 tctaagacta catctttgaa catagggcaa gtttatttag caacagatgt tattgtattg
    34981 ttgttatcat tattttatct atcagctttt gatatatttt actctttgat tgctgttaca
    35041 attagtggga aggtaattga ttttatttat tatcatggta aaagtttgga tatggatata
    35101 agtaatgata tagttccaga atgttaatat aaattaaata attttatatt ttattagggg
    35161 tggcaagttt gccacctttt ttgtgtctaa atatttacat aaagtataac ctcatctagg
    35221 tttttataaa tttcaaatat aaaattatgt tgaaatgact aggaatacga ttactagagg
    35281 tgctttttaa aaacccaatt ttatatttat gaaatactaa tcattaatgg atgtttctta
    35341 atcctatctt tatacctaaa atgataagat aaatgaagta gcaatacaaa actaaaattt
    35401 gtaaaagaga ttaaagtaaa tggaggagct aagatgttgg atatagttat gatagcattg
    35461 ttattcatag gatttgtttc catgaagtta cttgctgatt ggtgtggaaa acaaactgaa
    35521 aaaaaataat gtaatgtatg gagggattac ctatgtggtt tctggcagtt gtaattataa
    35581 tgttaataat atatttggta tatgcgctta ttaatcctga aaaattttaa acagtataag
    35641 actaataagt aagttgaatt ggggggaacg atttttatgg tgcagatagt aatagtgtta
    35701 gcgattttta tgattttagt aatacctatg ggcaagtatt tatatcatat agctactaat
    35761 caaaaaacat ttggagatag actatttgat aaagtggaca attttatata taaagtatgt
    35821 agtatagata agaaaaaaga aatgaactgg aaacagtatg ccttagcttt attatttaca
    35881 aatgcagtta tggtatttat aggatatatc atacttagaa ctcagagtat gcatattttt
    35941 aatccaagtg gaattaaaag tatggaacaa ggactatcat ttaatacaat aataagtttt
    36001 atgacaaata caaatcttca acattattca ggtgaatcag gactttcata ctttagtcag
    36061 atgacagtga taatatatat gatgtttaca tcagcagcaa caggatattc agctgctatg
    36121 gcatttgtaa gagggttggt tggtaaaaag aaaacattgg gaaattttta tgtagattta
    36181 attaggataa caacaagagt acttttacca ggagctttaa ttattgggat tatattagtt
    36241 acacaaggag taccacaaac ttttgctggg acagaaacgg tgactactat agaaggaaaa
    36301 cttcaagata tagctagagg tcctgtagcg gctcttgaat caataaaaca tttaggaaca
    36361 aatggtggtg gattctttgg ttcaaattca tcacacccat ttgaaaatcc aacaataata
    36421 tcaaatatag tagaaatatt atctatgatg atattaccag gagcttgtgt tgttgcattt
    36481 ggttatatga taaagaataa aaaacaaggt tgggttgtgt ttggtgcaat gtcaatcata
    36541 tttttaatag gtttagtagt ttgttttaaa gcagaaagtg caggaaatcc tattttatca
    36601 caattagggc ttaatcaaag tatgggaagt atggaaggta aggaagttag atttggtata
    36661 gcacaatcat cattattcac agcagtgact acttcattta caacaggtac agttaataat
    36721 atgcatgata cactaactcc tttaggagga ttagtaccac ttttaaatat gatgttaaat
    36781 gtagtctttg gtggtaaagg cgtaggtctt atgaatatgt tgatgtacgc tataatagca
    36841 gtattcctat gtggattaat ggtaggaaga acaccagagt ttttaactaa gaaaatcgaa
    36901 ggaaaagaaa tgaagcttat tgctttactt attatattac atccactttt aatactaatg
    36961 ttctctgggc tttcggtagc agtaccagca gggcttgaag gaatttcaaa tcctggattc
    37021 catggattat cacaagtatt gtatgaattt tcatcttcag cagctaataa tggttcagga
    37081 tttgaaggtc taggagataa cactatgttt tggaatatga caacaggtat agttatgttc
    37141 tttggaagat atgtgtctat tatagtatta ctagcaatat caagtttgtt agcatctaaa
    37201 aaagctgtaa atgaaagtat aggaacactt agaacggata attttacatt tacaatagtt
    37261 ttagtacttg ttgttttaat tgttggtgct ttaacatttt tcccagcctt agcgcttgga
    37321 cctatttcgg agcatctagt gttatggcac taaaaaattg ggagggagta aagttttatg
    37381 gatactaaaa aaacaaagtt tattacaaaa gatattttca aaacttccat tatagaagct
    37441 tttaaaaaat taaatcctaa atatatgatg aaaaatccag ttatgtttgt tgtagaaatt
    37501 ggatttatag taacattgat actcacaata gtacctagtt tgtttggtga tgaaggcaat
    37561 aatttaagag tttataatgg gattgtaaca ataatcttat ttataacagt tctttttgca
    37621 aattttgctg aggctgtagc tgaaggaaga ggtaaagctc aagcagatac acttaagaaa
    37681 actagaaaag atacagtagc aagggttata gatacgttag gaaatgaaaa aatgataagt
    37741 gcaagtgagc taaaacttgg tgatatcgtt ttagtaaatg caggagaagt tatcccaaat
    37801 gatggagaag ttatagaggg tatagcttct gtggatgagt cagcaataac tggagaatct
    37861 gcacctgtca tgagagaatc tggtggagat tttgcatctg ttacaggtgg tactacagtg
    37921 gtcagtgact ggctaaaaat aagaataaca acaaaaccag gagaatcatt cttagacaaa
    37981 atgatagctc ttgtagaagg tgcttcaaga caaaaaacgc caaatgagat tgctttaaat
    38041 actttgttag tagggttaac acttatattt atggttgttt taatatctct ttatccaatg
    38101 gcaaattatg caggtgtaaa gattccaatt tctactatga tagctctttt ggtatgttta
    38161 ataccaacta ctataggtgg attgttgtca gctattggta ttgcaggtat ggatagggta
    38221 actagattta atgtaattgc aatgtcagga aaagcagtag aagcttgtgg agacgttgat
    38281 actatgattt tggataagac aggtacgatt acttatggga atagacttgc atcagaattt
    38341 ataacagttg gaaacgcaga ttcaaaagac ttaataaatt attctgttat gtgttcatta
    38401 aaagatgata cgccagaagg aaaatctata gttgatttag gcatgaaatt aggctcaaca
    38461 ggtaagacaa aagaggcaga ggaagctgag tttgtagaat tttctgcaca gactagaatg
    38521 agtggtatta acctagctga tggtagagct ataagaaaag gtgcttatga tagcataata
    38581 aaaagagtaa aagaatctgg tggtatagta ccagaagatt tagaagaaaa tgtaaatcga
    38641 gtagctaaac taggaggaac acctttagta gtgtgtgtag ataatgggat atatggaatt
    38701 atctacttaa aggatacagt aaaatcaggg cttgtagaaa gatttgctag acttcgtgag
    38761 ataggaataa aaacaataat gtgtacagga gacaatcctt taacagcagc tacaattgca
    38821 agagaagctg gagtagatgg atttattgca gagtgtaagc cagaagataa aatagatgct
    38881 ataaagaaag aacaatctga aggtaaaata gttgcaatga caggtgatgg aacaaatgat
    38941 gctcctgcac ttgctcaggc agatgtaggt cttgcaatga atagtggtac aacatctgca
    39001 aaagaagcag caaacatggt agatttagat tcagacccaa ctaagatatt ggaagttgtt
    39061 gaaataggaa aacagcttct aataactcgt ggagctttga cgacatttag tatagcaaat
    39121 gacgttgcaa aatattttgc gattatacca gctatattca ctcttgcaat acctcaaatg
    39181 gagatattaa atgtaatgaa actttctaca tcatttagtg cgatattatc agctttaata
    39241 ttcaatgcta taattatccc atgtttaata cctcttgcaa tgaagggagt aaaatataga
    39301 cctatgaaat cacaagctat gttaatgaga aatatgctta tatttggtct tggtggagtt
    39361 atagtaccat ttttaggaat aaaattaatt gatttattga ttacaccttt agtaatggtt
    39421 cttaatttag gttaataaat ggaggatata aaaatgaaaa cattaaagcc agcattactt
    39481 gtaagtattg tattgttagt tgtttgtgga cttgtgtatc cacttgtttt gacaggtgta
    39541 agtcaagttg cattcaaaga taaagctaat ggaagtatga tagaagtaaa tggtgtaaag
    39601 gtaggttcag aacttattgg tcaaagcttt acagatgcaa gattctttaa aggaagagtt
    39661 tcatcagtaa actataatac ttatacaaaa gaagatttag taccagataa agatggaaat
    39721 acatcttatg gaggtgtatc atcagggtct tttaattatg gagctacaaa tccagaactt
    39781 cgtgatagag tgcaaaaaga tatagaacag tttctaaagg ataatccaac tgtcaaaaaa
    39841 gaagatatac caacagattt gcttacagca tcaggttcag ggcttgaccc taatataagt
    39901 ccggactctg caaaaattca gatacctgct gtagcaaaag cgtcaggaat atcagaaagt
    39961 aaattacaaa aaattgtaga tgataatact tccaaaaaag tttttggagt tttaggggaa
    40021 gatagagtta atgtactaaa ggttaatgta gaggtcgcaa gaatattagg tcttatatag
    40081 cattttgtag aattttagta acaaaatatc atgcaagatt taatttaaag gttagatttt
    40141 aaagagtgaa aatcgataat gattttcact tttttatttg aatattacta atatttggaa
    40201 taaagcgatt gtaagtataa aatccaaaaa aaggttgcat tatcaaaatg ataatactat
    40261 aatatattta tcgttatcaa tttgataata gattttgaca tataggagga aatagtatgt
    40321 tatataataa tgcattagag ttgataggaa acacacctgt agtaaggctg aataatttag
    40381 gttgtactaa cgggtgttct aacatatatg taaaattaga aaaagtaaat ccagcaggaa
    40441 gtataaaaga tagagccgtt tatgaaatgc tagaaggttt agagaaaaga ggagaattaa
    40501 aaaagggaga tgtactagta gaagcaacta gtggaaatac tggtattgca ttatctatga
    40561 taggaaaatt aaagggatat gaagtcgtaa ttgtaatgcc tgaaacaatg agtattgaga
    40621 gaagagattt aatgaaggct tatggagcaa atttggtact tacagatggt aaagctggaa
    40681 tggcaggttc catagaaaaa gcaaatgaac ttttaaaaga aaatccaaac tataaaagct
    40741 taaaacaatt tgaaaataaa gataatccaa atgcacatta taaaacaaca gcagttgaaa
    40801 ttatgaatga tgttaaagat ttagatatat ttgtatgtgg agttggaaca ggtggtactt
    40861 taataggaac tgcaagatat ttaaaagaac aaaatccaaa cataaaagta gttgcacttg
    40921 aaccaaaaaa atctccagct atatcagaaa acaagactgg acctcataaa atacaaggta
    40981 taggtgctaa ctttgttcct gaaaattatg attctagtgt agttgatgaa ataatattag
    41041 tagatgatga agatgcattt gatacagtta aattacttgc atcaaaagag ggaattttag
    41101 tagggatatc ttctggagca aatatatttg gtgctataga gatggcaaaa agatatccag
    41161 ataaaaagat agttacagta gctccagatg gagttgataa gtatatgtcg atgggaatat
    41221 tttaataatt ttatttgagg gagtgaatac tttgtttaag aaaataaata aagatattga
    41281 atatataatg aaaaatgatc cagcagctag gtctaagata gaagtttttt tactttatcc
    41341 atcagttcat gcaatgataa tgcataggat ggctcatgct ctttataaga agaagaagct
    41401 ttttacagca agattaatat cccagatatc cagatttatg actggtatag aaatacatcc
    41461 tggtgctaaa atgggagaag gtatattgat agaccatgga atgggtgttg taattggaga
    41521 gacagctgaa gtaggtaata gggtgacaat atatcaagga gctactttag gagccacagg
    41581 aaaagatact ggaaaaagac atccaactgt tggtgatgat gtattaattg gtgctggaac
    41641 aaaaatacta ggtcctttaa atataggttc aaattcaaaa attggtgcaa attcggttgt
    41701 cgttaaggat gtacctaatg gtgcaacagt tgttggtata ccagcaaaaa tagtaaaaat
    41761 aagaaatcta gaacctgtaa aaaagaataa aaaagaagta gactatgagt atgatgaatt
    41821 agataatgta tattatatat aggttttgta taaccataaa ataaacttta taaaaattat
    41881 ttttaaaagg tatttttttc aaatttgagg gcataataaa aaagaactga atgttacagt
    41941 tttggttcat caatttttga taaacaagat tttaaatttg tagcgtctag gaggttttta
    42001 ttatgaaagc aaatgttaat caagatactt gtataggttg tggattatgt ccatcaatat
    42061 gtccagaagt atttgatatg aatgatgatg ggaaatcaca tgttatagtt gatgaagtac
    42121 caagtgatgc tgaagaatca gcagcagaag ctagagaaag ctgtccagtt gatgcaatag
    42181 atgtgcatta ataaaataaa tgcctataaa aattgaattt tcaattttta taggcatttg
    42241 tcttttattc agtcagtatt tcaacacctg tttctgtaac taaaaccata cattcccatt
    42301 gagcagataa tttaccatct tttgtataaa tagtccaatt attttcttca tcaacgaaaa
    42361 tttcgtgagt tccttcattt accataggtt ctattgtgaa tatcatccca ggaactaata
    42421 acatctcagt ccctttttta gatacataac ttacaaaagg gtcttcgtga aagcttaatc
    42481 caactccatg accaccaatt tctctaacga cagagtatcc atttttcttt gcataagagt
    42541 tgacagcatc acctatatct cctaagaatc cccatggttt ggcaacctca agaccttttt
    42601 ggacgcattc ctttgtaact tgaactagtt tttttgcatt ttcgttaaca ttacctacca
    42661 taaacattct tgaagcgtct gaatagtatc cattaaaaat agtagaaaca tctacattta
    42721 taatatctcc atcttttaag ataatgtcct tatttggaat accatgacaa acttcattat
    42781 ttattgatgt acatacgctt ttaggaaatc cattatagcc tagtggagca ggaatagctc
    42841 cattttgtag tgtaaattca tgaactaact tatttatatc ttctgtagat attccaactt
    42901 cgattttatc tgaaatataa tccaaaattt ttgtgtttat tacagcactt ttttttattt
    42961 cctcaatttg actttcattt tttataatgt catgagttgg tacaatatga cctaatttag
    43021 caaatgaatc tattttatta tccatatcca tatgacattt tttatatttt ttagtacttc
    43081 cacaccaaca gctatcattt ctatttaact tcataacttt tcatccttct tttattattt
    43141 atttaaatta tgggttacat aaactatttt aaaacataac cattataaat attataacat
    43201 aaatactaaa aaatccttta tagatagtga tttattcaaa atataaatta tcatgtgatt
    43261 attgttgatg aaaagagtac agttaaatgt attattggat agtagtacac ttaatagtat
    43321 agttcaagtt tttcaagaaa atcatatgtc agaggacaat tttcctttaa agtatttttt
    43381 gaatcttcat caagataata gtgtacaaat gattctgcaa aaaattcttc tataaacttg
    43441 cttatatatg catgtgcaat ttgtttaggg tcataaagtt tgtatctttc ctgagcaaat
    43501 actttcataa actcttcaga atttgatttt ttatttaaaa cgtttttatc tatagcatgt
    43561 gcagtttcat gtaattctaa atttttagat gaatgtacat ttgtatagct aggattacta
    43621 taacctattt tagcaactat agaattattt ctatatatac caggtacatc tttccaagtc
    43681 ttgcctgatc ttacccagca tggaggtaat tgatatttta aatcactaaa ttctggttca
    43741 tcagttagat ttgaatttat taattttatt tttatatcct tttctttaag tgcttgtaaa
    43801 tatttaatat ttatttttgc aagtctgctt ttcatatttt caacttcatc tttatcataa
    43861 ttcaagtcat caggaagtat aagcatttgt tcaattatat ctatgttact gtcttttata
    43921 tcttcagact cttcatcaaa tttatattga agattatcat tttcatagat agttatattt
    43981 gtattatttt ccatagaata agagtgaata tcctctaagt ttaaaatatt catttgttgt
    44041 aaaatatgtt ttgtttcgcc actccatact actaaagaaa gtgaattaaa attaattagt
    44101 ggattttctt ttgcttttat atcattgctt atatcgacat caaatacata ttcaattgag
    44161 tcctctatgt taaggccatt tgtattcttt ttaatataca aatacgcttg ttttaagtct
    44221 atataaatct catctaaaga ttcgtcataa aattctgata aattattagt aaagagttct
    44281 tcacttaaaa gttcattttt atcagttata ctttcgatta aaatattaat ttcttttatc
    44341 atatcgtaaa tacttatttt tctatttgct tcttcactag atacattgca tggcgcaaga
    44401 gcaatgcaat aaaaatcttt ttctttttta taaaaggctt taagtgattt tgataaaagt
    44461 gtaaagtcta cacctatctg taaatttacc ttaagtgaat cattttgtga taattcagta
    44521 ttatttcttt tagctgtatt taaattattt tccataatca ttgccccctt tttattaggg
    44581 tatcataatt tttttatatg tgtcaattta tttttttgaa aattataatt tttttgaaaa
    44641 ttttattttt tataatttgt taatttttta ttatagaa
//
LOCUS       DAMJVM010000022        44019 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.022, whole genome shotgun sequence.
ACCESSION   DAMJVM010000022 DAMJVM010000000
VERSION     DAMJVM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44019
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.022"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(34..129)
                     /locus_tag="PC523_001312"
                     /pseudo
     CDS             complement(34..129)
                     /locus_tag="PC523_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
     gene            complement(223..1167)
                     /locus_tag="PC523_001313"
     CDS             complement(223..1167)
                     /locus_tag="PC523_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HDF2474851.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVED
                     GHVFYNVMPSLNHNHMIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFIKKYEKNEQFSFEICDTHQELIVDSDVVISTVTYTDEYLSEYKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQGKGRENDDERIISY
                     NIGLSIHDILFANKIYELCEILNCEEIELKEPKEKYWI"
     gene            complement(1187..2419)
                     /locus_tag="PC523_001314"
     CDS             complement(1187..2419)
                     /locus_tag="PC523_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDF2474852.1"
                     /translation="MKRLMILGSIKYFENIVMSAKKEGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKSIAIDKKIDGILTGFTDSLMKPYVYVANELNLPCVISCNQLESVT
                     NKAVMKKYFKNNGIPTTDYCIVESLKDLEKIQELNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEVSQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLIYPSKYCFQYEEEIKSIYQNITQAFDIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEINFDEIKKYNMHLSKCVYALPIYIKPGTI
                     KKINNVDKIESLDYVTQILLLVKEGDTIGLYEDERQDCGRVYGIANNIFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            complement(2511..3506)
                     /locus_tag="PC523_001315"
     CDS             complement(2511..3506)
                     /locus_tag="PC523_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HDF2474853.1"
                     /translation="MNNIRINKFEKSCEKHWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIIYKGEEILAVVPACEIYENGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLQEQSFNKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DIVANFRYSKRRDFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSIEEL
                     LDFKNNRLKDIVEFYAVYIDGIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKNALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            complement(3515..5629)
                     /locus_tag="PC523_001316"
     CDS             complement(3515..5629)
                     /locus_tag="PC523_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2474854.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIYDILEIFKKNADVGMIGVAGVKDI
                     PVNGIWWDSDKKVGKVYDSHTGKMDIYEFSNFDECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIIPKQNKPWCMHDSGVANINNGYDEYRIRFVKEYI
                     NLDLDRENFYEFDEKKASTILKSNKHVMDCMKEELTEQLILKNYKDVLDIVSKIARFS
                     VFNHTGLYSCEKCERALIECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPEWLLKAIDDSGGEIISLDLISDDYLKRAMALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGASIADIVIDFSE
                     ASQLITLNKRGIDKSEILPILLKEKEVFSKSEIRKQYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENIYWKTLSNVTDNRVICFGTTTNIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDRFDYKNIKEVID
                     MIKRYNTSEFDRNKIKEKQSGIKHFVIDKHCIEIKNYIDKIYNEANHHKVKCDLYKND
                     EIEEYQLFVALYGYMVKNKNMS"
     gene            complement(5622..7499)
                     /locus_tag="PC523_001317"
     CDS             complement(5622..7499)
                     /locus_tag="PC523_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2474855.1"
                     /translation="MNTPILVNEKSKDIIKDWQYTYTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKDKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNSKNLGFPKGCNQGIE
                     LANGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSVE
                     EMFIFAQKINKSNINLWKYRLKLVGFCMLIKKSVIDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCKDTFIHHFGSISFKENRNKYTDIMDINRRKFETKWGFNPEYSGIIRT
                     DLISKIDDSPETNINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIADIEKVKLDYKKNFFDYIILGDVLEHLIDPWKIVNKLKQYLKKDGYLVSSIPNVMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITAISMPLSEID
                     NRFIDTMCKESCKEMRQQYESYQYIVKATKSIDTKKYSSISMVNLKYALMRIDNELDI
                     DGNLSYIFDNYNITEPCFVDDIEYLVDTFVINKVTLLNRLGVEAFNRGLGDFSINMFM
                     KVLEMDRDDIDSIYNITSVLCELEEFDIAYNLIKNSSEKVQSESDIQEILYFIEGKRY
                     E"
     gene            complement(7523..9664)
                     /locus_tag="PC523_001318"
     CDS             complement(7523..9664)
                     /locus_tag="PC523_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2474856.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRDSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFEELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLESAPGYLITSLVKTYLYLGKHKKCEKLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLERYESVISEYNNIEDIEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YRNIEEVINNIKEDEKEYVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKIAFEEKLDLLDIFYEMEYVWIEKYLKYLLALERSFNLALYKYIISCPNTSDM
                     EKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYSNLSDFELIKFVCND
                     LDKFTIEFKHLLNIKNKVNSANEKLEYIHSMRKLLNEYPFYNKIIKILIGELEENIKE
                     SEEFRDLKDSFIISIENMIETGKINDAKLLVDDYSKTFVDDVKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            complement(9757..10629)
                     /locus_tag="PC523_001319"
     CDS             complement(9757..10629)
                     /locus_tag="PC523_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HDF2474857.1"
                     /translation="MRVNTNVSALIANNQMGRNVSAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTTN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNVDGASGTDAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            complement(10730..11065)
                     /locus_tag="PC523_001320"
     CDS             complement(10730..11065)
                     /locus_tag="PC523_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HDF2474858.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISSKLDERQEIINSV
                     SEIDRNDFIQLYNKMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYSLNRE
                     KVNIFNKKV"
     gene            complement(11068..12591)
                     /gene="fliD"
                     /locus_tag="PC523_001321"
     CDS             complement(11068..12591)
                     /gene="fliD"
                     /locus_tag="PC523_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDF2474859.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNIIQESKDLYDKYLSVDSPNSIVSQKAYSSTKITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVQQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FVSDAGGNKLASVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMKSFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKSLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            complement(12612..12968)
                     /locus_tag="PC523_001322"
     CDS             complement(12612..12968)
                     /locus_tag="PC523_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HDF2474860.1"
                     /translation="MELNLAELGKLSKEELLLMLVDATVKYTNISKDALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQDAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNKIKI"
     gene            complement(12968..13363)
                     /gene="fliS"
                     /locus_tag="PC523_001323"
     CDS             complement(12968..13363)
                     /gene="fliS"
                     /locus_tag="PC523_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDF2474861.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIARAAILD
                     KNTQRAHRELVRVQDIFTELMVTLDQNAGQWAKDMYKVYDFVRYELSRANIRKDIQII
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            complement(13385..13597)
                     /gene="csrA"
                     /locus_tag="PC523_001324"
     CDS             complement(13385..13597)
                     /gene="csrA"
                     /locus_tag="PC523_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDF2474862.1"
                     /translation="MLVISRKKDEAVLIGENIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNINILKSLK"
     gene            complement(13591..13983)
                     /locus_tag="PC523_001325"
     CDS             complement(13591..13983)
                     /locus_tag="PC523_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HDF2474863.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDSLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVMLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDSGC"
     gene            complement(14000..14932)
                     /gene="flgL"
                     /locus_tag="PC523_001326"
     CDS             complement(14000..14932)
                     /gene="flgL"
                     /locus_tag="PC523_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDF2474864.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMEIYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKIKEIANALNATHEGKYIFSGSNTGIPPVECVDNADGSVSLKFN
                     SSIDLNKLKDSLSMDVAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGEMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKEILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFSVSILDYI"
     gene            complement(14951..16261)
                     /gene="flgK"
                     /locus_tag="PC523_001327"
     CDS             complement(14951..16261)
                     /gene="flgK"
                     /locus_tag="PC523_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDF2474865.1"
                     /translation="MAGLFSILNTANSGMNSHQKAIQTISHNISNLDTEGYSRQRTEF
                     ATNSPMYMPSLSNSIGRGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKGDIDSNIDTEIKQINEMLKSLEDLNKSIDIISGSGSMPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNADGTWKTGVSGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVRDFFVTSNVDGEDIIKVNPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAELVGLDSKKVNQDEVNQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            complement(16279..16695)
                     /locus_tag="PC523_001328"
     CDS             complement(16279..16695)
                     /locus_tag="PC523_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HDF2474866.1"
                     /translation="MITDLKVIMYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSKDGYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPVDKQTTTYNSYGNIRK"
     gene            complement(16700..16975)
                     /gene="flgM"
                     /locus_tag="PC523_001329"
     CDS             complement(16700..16975)
                     /gene="flgM"
                     /locus_tag="PC523_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HDF2474867.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGVKKVEDVKKINKTDKIELSKFAK
                     EMASVSNMSDESIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            complement(17127..17441)
                     /locus_tag="PC523_001330"
     CDS             complement(17127..17441)
                     /locus_tag="PC523_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2474868.1"
                     /translation="MNSKKEDIYELNFGQLIEEQSGIIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLNKNVDEKLDIYANDRLFAYGESIITNEKLSVRLSKIEDSD
                     IN"
     gene            complement(17465..17893)
                     /locus_tag="PC523_001331"
     CDS             complement(17465..17893)
                     /locus_tag="PC523_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HDF2474869.1"
                     /translation="MGINIDKLKISPSINIEVKKLKENNKFSDRLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            complement(17902..18570)
                     /locus_tag="PC523_001332"
     CDS             complement(17902..18570)
                     /locus_tag="PC523_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDF2474870.1"
                     /translation="MNEISSLINILALTQLSGSNNTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNGYNISSNSENLFNELENVVNNTTSRFIDVDTKDKNVKLRIENAVEQA
                     SKKYNVDANLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            complement(18608..19591)
                     /gene="rfbB"
                     /locus_tag="PC523_001333"
     CDS             complement(18608..19591)
                     /gene="rfbB"
                     /locus_tag="PC523_001333"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDF2474871.1"
                     /translation="MKKILITGGAGFIGSNFVHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVRGDISNREQMYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSPINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLKVVKIILNLLGKSEDLISYVND
                     RPGHDLRYAIDASKIQNELGWKAKYDFDFGIKETVKWYIENESWWKSVLSV"
     gene            complement(19629..20186)
                     /gene="rfbC"
                     /locus_tag="PC523_001334"
     CDS             complement(19629..20186)
                     /gene="rfbC"
                     /locus_tag="PC523_001334"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDF2474872.1"
                     /translation="MGKINVIKTKIEGLYIVEPSVFCDERGYFMESYNKRDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVRYPQGKLVRVTEGEVFDVAVDLRKDSKTFGEWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFNYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICIRR"
     gene            complement(20235..21104)
                     /gene="rfbA"
                     /locus_tag="PC523_001335"
     CDS             complement(20235..21104)
                     /gene="rfbA"
                     /locus_tag="PC523_001335"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDF2474873.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLKITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLEKAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYKKGYISRAQLMELAKPLIKTE
                     YGQYLLKLASEDM"
     gene            complement(21213..22085)
                     /gene="rfbD"
                     /locus_tag="PC523_001336"
     CDS             complement(21213..22085)
                     /gene="rfbD"
                     /locus_tag="PC523_001336"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDF2474874.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSMSQPKYAILATTRNDFDI
                     YNQTNVDNFILNNKPDVVVNCAAYTKVDACEDNIETAYKVNALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDSKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLTKIIIRIMETEHYGVYHATCEGE
                     CSWYDFAKKIFELKNIDIKVNPIKSNEFKCKAQRPLYSVLDNFMLKLIGLNSFRNWEE
                     SIEEYLERYDMNEE"
     gene            complement(22289..27370)
                     /locus_tag="PC523_001337"
                     /pseudo
     CDS             complement(22289..27370)
                     /locus_tag="PC523_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725947.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
     gene            complement(27381..28913)
                     /gene="purH"
                     /locus_tag="PC523_001338"
     CDS             complement(27381..28913)
                     /gene="purH"
                     /locus_tag="PC523_001338"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDF2474875.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKAKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            complement(28906..29499)
                     /locus_tag="PC523_001339"
     CDS             complement(28906..29499)
                     /locus_tag="PC523_001339"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDF2474876.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQDAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            complement(29493..30569)
                     /locus_tag="PC523_001340"
     CDS             complement(29493..30569)
                     /locus_tag="PC523_001340"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDF2474877.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHEIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVNERELHKSFNMGIGLVLIVDKANADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            complement(30563..31930)
                     /gene="purF"
                     /locus_tag="PC523_001341"
     CDS             complement(30563..31930)
                     /gene="purF"
                     /locus_tag="PC523_001341"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDF2474878.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKESELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            complement(31942..32640)
                     /locus_tag="PC523_001342"
     CDS             complement(31942..32640)
                     /locus_tag="PC523_001342"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDF2474879.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            complement(32646..33122)
                     /gene="purE"
                     /locus_tag="PC523_001343"
     CDS             complement(32646..33122)
                     /gene="purE"
                     /locus_tag="PC523_001343"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDF2474880.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     STFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            33572..34084
                     /locus_tag="PC523_001344"
     CDS             33572..34084
                     /locus_tag="PC523_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2474881.1"
                     /translation="MLEVIKNRRSIRAYINKSVEEEKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKKLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWVGVFPKNKVVNKVRQTLDLPVKLVPYSLISIGYSEEKNEFIDRFDEN
                     KIHRNIYKNR"
     gene            34634..34849
                     /locus_tag="PC523_001345"
     CDS             34634..34849
                     /locus_tag="PC523_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474882.1"
                     /translation="MRILSETEKISLAAVIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
     gene            34855..35148
                     /locus_tag="PC523_001346"
     CDS             34855..35148
                     /locus_tag="PC523_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HDF2474883.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWFKPYDKPIQQLTCSYKESQNVID"
     gene            complement(35330..36043)
                     /locus_tag="PC523_001347"
     CDS             complement(35330..36043)
                     /locus_tag="PC523_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2474884.1"
                     /translation="MINLIFDVDDTLYNQLTPFYIAYNKVFSSIKNISIEDLYMSSRK
                     YSDEVFHMTENGEMAIKEMHIYRIMKAFEELGTSITEKDAQSFQDEYIYQQSQITLIP
                     EVEWVLNFSKERNINLGIITNGPSTHQRMKLKQLNIENWVDKNNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYMGDSYRNDVLGAKKAGWKSIWLNHRRHEVEGVFYKPDFL
                     ILEHKDLMSLFIKICSKKC"
     gene            complement(36131..37396)
                     /locus_tag="PC523_001348"
     CDS             complement(36131..37396)
                     /locus_tag="PC523_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HDF2474885.1"
                     /translation="MKKLPIKTVVQRLLELQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKIADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPEIGYEVGTEETNGGLTSTETYETFITRLKVELDKRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SSIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVDKTSNIRHVLLVNAIECGRWRK
                     WVIGEQKNLTTEDIFKNEVLSNEILDIAGHYTFNNDDVKKEIEVLYANLSKNNIDGQR
                     FVIDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            complement(37500..37808)
                     /locus_tag="PC523_001349"
     CDS             complement(37500..37808)
                     /locus_tag="PC523_001349"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDF2474886.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKEIEEATCVILTNDVEIRNMERFKGKKVVRMSVSDIIKKSDALIKKIKDTFQ
                     "
     gene            complement(37879..38934)
                     /locus_tag="PC523_001350"
     CDS             complement(37879..38934)
                     /locus_tag="PC523_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDF2474887.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLIGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMVGSI
                     IGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAILMKLVKKDVKEDE
                     EIEEDLDDSIELIFEEL"
     gene            complement(38936..39388)
                     /locus_tag="PC523_001351"
     CDS             complement(38936..39388)
                     /locus_tag="PC523_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2474888.1"
                     /translation="MSKLVNVKCSFKIHANSKEEAIISLVDVVSKEGYLRDKNQFLED
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEGED"
     gene            complement(39385..41415)
                     /locus_tag="PC523_001352"
     CDS             complement(39385..41415)
                     /locus_tag="PC523_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2474889.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKPVIQKMNKGVLIIPDNFQVVCINNNEYVFSLTERVSIMKIISMFKIENLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKAFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGISLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESVSLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLNQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLINDYQINIVDSIPSYKLQDYKDWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     VICQELGYSDERALSRQLNLGDLIGIDTIRIIDEDMKWEDAVKISSNILANCDFVSSQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVRIIF
                     CLASKDKKEQIPAIINLTKMVYKTNFISMLESAKTKEEAEGLIHKYEKEVTV"
     gene            complement(41680..43908)
                     /locus_tag="PC523_001353"
     CDS             complement(41680..43908)
                     /locus_tag="PC523_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2474890.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISSKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDIGI
                     IDLEGKAEFLSGKKFDLKNTDSYKKGIEGKEYSEVIESMGFVFYSVPIFNDNHEVSSI
                     LIGVSNKESMDEILNIDNFNGEGTIEIINSQGRPLFIGKNTELIKDLGHKYKSSGDEP
                     WAQKMLNDFKNDKSGNITITSSKGVQCLLTYYPITKGLNDWHFVLIVPEDAVLGELNK
                     LNSFTIIMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSILVKP
                     LILESPDSTYMMVAINIKDFKLINDCFGSKKGNLTLKHLYNVLYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADINYFNKNRENPYFLRISAGIYTIENHNEDLIT
                     IQDHANTARKSFNKAHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKLICNKYNVPTYLIDLELTESIFLENPEAVD
                     IIDDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFEDFAYENNKIK
                     NI"
BASE COUNT        13848 a         7987 c         4416 g        17768 t
ORIGIN      
        1 actaaagtca caagtgtgcg tagctatttt ttaatcaaaa ggtattactt tatttgattc
       61 taatgcaact aaatttccta tatattcatt tccaatagat acgaacttaa ttaaattata
      121 tttatccata aatttatcta tcctattcat actaattttc taacaatata ttacttcata
      181 ttcatattta aaataaatta ccataaatat gaatgaaata aactatatcc aatatttttc
      241 ttttggttct tttaattcta tctcttcaca atttagtatt tcacacaatt cgtaaatttt
      301 atttgcaaat aatatatcat gtattgacaa acctatattg taagatatta ttctctcatc
      361 atcattttct cttcctttac cttgtttact taaaacatca taaaattcac aagaatactt
      421 atatctatct atattttcta ggtgtttcaa atgattataa tcatctaaat ataccttatc
      481 aaatagataa tcaacatttc caaatcccat aacatgtaca ggtaataata aagcaccttt
      541 tttaaaccat ttatattctg ataaatattc atctgtatat gtcacagtag aaataactac
      601 atctgaatct acaataagct cttgatgtgt atcacatatt tcaaaactaa attgttcatt
      661 tttttcatac ttttttataa atgactctgc ttggtcttta tatttaagta actttatatt
      721 tatattttta tctttaagag tatacgaaag catattcatt atagcagaag cagttacacc
      781 taaacccata atagcaatag tattaaaatc ttttttagca aatgtatcaa tagccaaggc
      841 agcaacagca gcagtcctaa aagctgtaat ataattacca tccaaaatag cctttaattt
      901 agcatcttcc atattatata gaaatatctc tgaatttaaa cttggatttc tccctggcaa
      961 ccttgtaacc atttttattc caccaatcat atgattatga tttagggaag gcattacatt
     1021 gtaaaataca tgtccatctt ctaccatgct tatttttggt ggtaattgac aatctttttt
     1081 tgttaaaaag gattctttta tccactcata cccttgtttc tgagaaatgt ttaatgacat
     1141 aatttgttca tgagttatca gcttcatatc tatcatactc ccttatttaa aaattttcta
     1201 tcatattctc attattttca tcaagtattt ctatcatttc atgaatacat ttcttcttta
     1261 tatttgcatc aaatatatta tttgctattc cataaactct tccacaatcc tgtctctcat
     1321 cttcatataa tcctattgta tctccttctt ttactagtaa tagaatttga gtgacataat
     1381 ctaagctttc tattttatct acattattta tcttttttat tgtcccaggt tttatgtata
     1441 ttggtaatgc atatacacat tttgatagat gcatattata tttctttatt tcatcaaaat
     1501 ttatttcatt attcacacta aagtttgcaa ataagcttcc tatgtctaaa tctgttattt
     1561 gagaaatagc catatagtca caacctccag gtaatcttgg cattacttca gataccttta
     1621 ttccatatgc tcccactaac atttgtacat aaagaggtcc attttttata tcaaaagcct
     1681 gtgttatatt ttgatatata gattttattt cttcctcata ttgaaaacag tactttgatg
     1741 gataaattaa tctatctggt tttccagctc ttcctgtatg tatatttaca agctctctat
     1801 ctccaatata aaatacatga gactctccat tatgaaccca agctagacct tgtatttcct
     1861 cactttcata aaattcctca attatgactt gtccatcttg tgaaacttcc ttagaatatt
     1921 caaacttttc tattaattct tctatatttg atacaaaata tacacccctt gaaccatagc
     1981 catcaacagg ttttattacg agtggaaaat ttaattcttg tattttttct aaatctttta
     2041 gactttcaac aatacaataa tcagttgtag gtattccatt gtttttaaaa tattttttca
     2101 taacagcctt attggttaca gattcaagtt gattgcaaga aattacacat ggtaaattta
     2161 gttcatttgc aacataaaca tatggtttca ttaaagaatc tgtaaatcca gttaaaattc
     2221 catctatctt tttatctata gctatacttt ttattttatc aaaatcaaat acatcaacat
     2281 ctattgcttc atcacaaatc tttttagcag gagtatcaat tctattatca caaacaattg
     2341 tgtatattcc ttctttttta gctgacataa caatattctc aaaatactta attgagccta
     2401 aaatcattaa tcttttcata tataaacctt ctttataaac taattatttt tatctaagat
     2461 tagagtacta tatatttact atactctaaa aactctctaa aacattatta tcacttattt
     2521 atatttttat agtacgttct gttgcaaatt gcacttgagc caaaaccttc tttaaatgta
     2581 gctaaaccct cgtctaagta tttacctcta tttagagtgc taattccaaa agaaagatac
     2641 ttaaaatttc tctctaaagc attttttatt agattataat ataaaaatat cattggataa
     2701 tatttattat atgtcgaatt tgatgctaga tattgagtat gataaactct ttcttgaaag
     2761 ttaaatacca tactcccaca aatcataatg ccatcaatat atactgcata aaattctaca
     2821 atatctttta acctattatt tttaaagtcc agaagttctt ctattgaatg tattacttta
     2881 gctcctttac tatctaaaca attagaaagt aaattaaaaa aacaaactat atcttcatca
     2941 ctatacagtc ttttaaaggt aagattgttc ttcaaagaat atttaaaatc tcttctttta
     3001 ctatatctaa aattagctac tatatcttct ttatacttac ttaattctaa aaacatactt
     3061 aattcatcat aaaacatgta attattctta aatagaaaat attctagtaa tgaagtatcc
     3121 tccttacaaa atattcttga tgtatttttt aaatatactt tattgaaact ttgttcttgt
     3181 aaatatattt ctaacttatt tgtaatagca tttacatgcc taatatcatt aaaaccactt
     3241 cttattataa tacctccaaa agtgcttcct aaatgagaaa aaaatattct ttcaccattt
     3301 tcataaattt cacatgcagg tacgactgct agtatttctt cacctttata tattattaat
     3361 gagtggtcag taaattttcc ctcttcatga taattaagga aatttctagt ttgcagaaat
     3421 gtaccattta tagatttatt ttctacaaaa taatcccagt gtttttcaca gcttttttca
     3481 aatttattga tacgaatatt gttcatttta ccacctatga catgttttta ttttttacca
     3541 tataaccata taatgctaca aaaagctgat attcttcgat ttcatcattt ttatataaat
     3601 cacacttaac tttatgatgg ttagcttcat tatatatttt gtctatatag ttttttattt
     3661 caatacagtg tttgtctata acaaaatgtt ttattcctga ttgtttttct tttatcttat
     3721 tcctgtcaaa ttcactagta ttgtaccttt ttatcatgtc tataacctct tttatattct
     3781 tataatcaaa cctatctata aaattccaca tattcgtaaa aattggtgaa acttcatttt
     3841 taaacttaac aggaattaaa tcatataaaa ttgcatctaa ttttgaagtg tgacttccta
     3901 ccatgaaaga ctctagatat atatccgaaa tcttataaaa atcgtctata tttgttgtcg
     3961 tcccaaaaca tataactcta ttatctgtaa cgttacttag agttttccaa tagatattct
     4021 ctgtatcaac tcctacaaca ataataattg tattatccgt attttcaatc aatgttttag
     4081 caattttaaa gtaattaata tcatttacac tattgtattt tctttcacta gcaatagtca
     4141 atattattgt cacatcatcc tccaaattat attgttttct aatttcagat tttgaaaata
     4201 cttctttttc ttttaataga attggtaata tttcagattt atctatacct cttttattca
     4261 atgtaattag ttgactagct tcactaaagt caataacaat atccgcaata cttgccccta
     4321 accaaaaagt gctgtcataa tgattaacat gtaaaatagg tattttccca tcaactccaa
     4381 aagcaacaac aggtattgta tcccacatat gaacatataa aataactata tcagcccagt
     4441 cataagctat ttttctcaaa gccatagctc tttttaaata atcatctgaa attaaatcta
     4501 aagatataat ttcgccacca ctatcatcta ttgcttttaa taaccattct gggagtgtgt
     4561 ctgattgcca tgtagttaca actgaatgaa cagagtcttt gtcaatctca atccagtttt
     4621 taactagtct tgtatgacct cctgttgaat atccttcact taaaacatgt aatacttttc
     4681 ttctttttaa atcttttttt gtaaataatt tcgtattttc tatttctggt aaattatttg
     4741 cacattctat taatgctctc tcgcattttt cacaagaata cagtccagta tgattaaaaa
     4801 cagaaaaacg agcaatttta gaaacaatat ctaaaacatc cttataattc tttaatatta
     4861 attgttctgt aagttcctct ttcatacaat ccattacatg cttattactc ttaagtatgg
     4921 tacttgcttt cttctcatca aattcataaa aattctctct atctaaatca agatttatgt
     4981 actcttttac aaatcttatc ctatattcat catagccatt atttatattt gctactccag
     5041 aatcatgcat acaccaaggc ttattctgtt ttggtataat aactttgtat ttattttgtc
     5101 taaactccat actctgtgac aaatcataaa aatgccatcc atcaaactta tcctctctcc
     5161 agaatacatc atattgagta atcataatca atccatctat agccataact tcattaaaac
     5221 attcatcaaa gttactaaat tcataaatat ccatcttacc tgtatgacta tcatatactt
     5281 tccctacttt tttatcagaa tcccaccaaa ttccattcac aggtatatcc tttactccag
     5341 ctacaccaat cattcctaca tctgcatttt ttttaaatat ctctaaaata tcatatatga
     5401 aatttttatt aattataaaa gtatcttgat gtaaatatac cttatactta gaatctgttt
     5461 ctctcatagc tttattataa gcagaggcaa tagaatcggc tcctcttaaa gctactgttt
     5521 ctatactaaa gtttttagga atatctaggc tatttatata tacaagacat tcttcataaa
     5581 gtctttcatc atttacacag gttataaaac atatcttatc attattcata tctcttcccc
     5641 tcaataaaat aaagtatctc ttgtatgtca ctttcactct gtactttttc gctactattt
     5701 ttaattaaat tataagcaat atcaaattct tctaattcac ataatacact tgttatatta
     5761 tatattgagt caatatcatc tctatccatc tctaatactt tcataaacat gtttatacta
     5821 aaatctccta gacctctgtt aaatgcctca actcctaatc tgtttaatag agttacttta
     5881 tttatcacaa atgtatcaac taaatattca atatcatcaa caaaacatgg ttcagttata
     5941 ttataattat caaatatata acttaaatta ccatctatat ccaattcatt atcaattctc
     6001 attaaagcgt attttaaatt aaccatacta atactagaat attttttggt atctatgctt
     6061 ttagtggctt tcactatata ttgataagat tcatactgtt gtctcatttc tttacagctt
     6121 tccttacaca ttgtatcaat aaatctatta tcgatttccg aaagcggcat actaatagct
     6181 gtaatactat ttattatgta attactatca ttaaacattt tcgttatctc atttaatgta
     6241 aaaaacctta aatgggtctt gtccagaatc cctgaatctt catatgtaaa attacctaac
     6301 acaagatttc ttaaaacaga aacatgcata acatttggta tactagatac caaataacca
     6361 tcctttttta gatattgttt caatttattg actatcttcc atgggtcaat taaatgttca
     6421 agtacatctc ccaatattat ataatcaaaa aaattttttt tataatccaa ttttactttt
     6481 tctatatctg ctattagcac gtctgcacta tattttgcaa gtttaccagc tgactcacta
     6541 atttctattc catatattga tgcattttta tatatatttt ttatttctaa aagagtagct
     6601 cctataccac atccaatttc taaaacattg atatttgtct ctggactatc atctatttta
     6661 gaaatcaaat ctgttcttat aattccacta tattctggat taaatcccca cttagtctca
     6721 aattttcttc tattaatgtc cattatatct gtatatttat tgcgattttc tttaaaagat
     6781 atacttccaa aatggtgtat aaaagtgtct ttgcataata ataacttata tcctgactct
     6841 atcattctaa aagataaatc atcatcttca aaatttccag gcgtgaatag ttcatctaaa
     6901 actcctacat tatctattac agattttttt attaacatac aaaaaccaac taacttcaat
     6961 ctatatttcc atagattaat attagattta tttatttttt gtgcaaatat aaacatctct
     7021 tcaactgatt tgtagtcaac atcaatctgt tgatagtatg aacatgcatt tgttattgga
     7081 cctacagctc ctatattatc ttgtgaccat aaagccttat tcaaattata aagccaattt
     7141 ggagtaacta ttacatcatt atttaacaac aatatatttt caccattagc caactcaata
     7201 ccttgattgc aaccttttgg aaatcctaag ttcttgctat tatacacaga ttttatatca
     7261 gattgtccct ttaaccactc aactgttccg tcagaagagt tattgtcaat tattattatt
     7321 tcatatttat cctttggggt aaatttccta atactttcaa tgcataactt agtatattct
     7381 atttgattgt atgtcaatat tattatgctt gtaaatttta tatcttcatt tgtatatgta
     7441 tattgccaat cttttattat atctttagat ttttcattaa ccaatattgg tgtattcatt
     7501 cttcaattct cccatttttg acttatctta aactttttaa atattctata ttatatatag
     7561 tgtcttcatt ttctacatct aatgataatg ccttttttaa cataaagtca gctttgtcaa
     7621 gcttattttc aaacatatat aaaattccct ttatgtttaa aatctttaca tcatccacaa
     7681 atgtttttga gtagtcatct accaataatt tcgcatcatt tattttgcca gtttcaatca
     7741 tattttctat actaataata aaactatctt ttaaatccct aaactcttca ctttctttta
     7801 tattttcttc taattcacct ataagtattt ttattatttt attataaaaa ggatactcat
     7861 ttagtaattt cctcatacta tgtatatact ctaatttttc attagcacta tttactttat
     7921 ttttaatgtt aagtagatgc ttaaattcta tagtgaactt atctaaatca ttacaaacaa
     7981 attttattaa ttcaaagtca cttaaattgc tatatatgta tcttatgtac ttataagaat
     8041 acattatata aagataaaaa atttccttat atttttcatc acttaagctt atactttcta
     8101 aaactacttt agatatagct ttatatactc ttatcttttc catatctgat gtatttggac
     8161 aactaattat gtacttatat agcgctaaat taaaacttct ttctaatgct aaaagatact
     8221 ttagatattt ctctatccag acatattcca tttcataaaa aatatctagt aaatcaagct
     8281 tctcttcaaa agctattttt ataatatatg cataaattat ttccttttct tcattgagta
     8341 tttctttaca tttatctata gatacacatt tatttatcct tatctgattt aaaatgccat
     8401 aattaccttc aatgtcacaa agaattttat atacatactc tttttcatct tctttaatat
     8461 tatttataac ttcttctata tttctataaa aactattttt ttcaattttt gaatttgaaa
     8521 cttctttata atagtttttt atatcttcaa atctattcaa tttatatagg gacataaata
     8581 aactaaagta cacattcttt ttcttttcta tatcttcaat gttattatac tctgaaatta
     8641 cagactcata tctttcaagc ttataataaa tctttataag tgtaataata gcctcatccc
     8701 taagccctac agtatcagtg tctgaaaaca aactatttgc ttgtgtactt atttcataat
     8761 tatctaacaa ataaatgtat ctcttatacg aatctatact attttcatac ttccctaaat
     8821 ctacttgtgc ttgtgcaata taataatata tatcaatatt attcatgtct ttcttaatat
     8881 actttagaca caatttttca cacttcttat gttttcctaa atataaatat gtttttacaa
     8941 ggcttgttat caagtaacca ggtgccgatt ctagtttaga atataattca taagacttct
     9001 caagataata taaagcctcc tgatattttc ctaatataat aaaatttttt cctaattgat
     9061 aatttgtata tggagaatta tcattctctt ttaactcttc aaataatagt ttttcatttc
     9121 tttttacttt atttaatctt atttcttcat cttcaaacaa atatccataa tgttcaaagt
     9181 cagcaacatt attaaatata gggtttttgt atattggttg ttcatgtact ttaccatggt
     9241 atctaaaatc aacgttttta aaaagtctaa gcactgaaca atatccatat aattttttat
     9301 catatgagta aagatttttt aattgtattg aagcagaatt aaatttttta tcaagaccat
     9361 ctttaaaaaa tttcacaata gttgaagaat caatcagtat ttcgtctgca tctaatataa
     9421 gtaaccattc gcccttggca taggatattg atatatttct catactagaa aaatcattat
     9481 tccaattatg aaaatacact ttttctgtgt actttttaga tatttttata gtatcatctg
     9541 tagaacctgt atcaactatt ataagttcac tctctataga atctcttaat gtggttaatg
     9601 attttaaagt tttttcaagt attttttctt catttttaac aatcataact atactaagca
     9661 acatatatat tttcctttca aaagtatatt tttaaaaaaa gaaaggagta aagcaaagcc
     9721 ttaccctttc ttctatatcc aatatacaaa attattttat cctaataatt gtaaaactcc
     9781 ttgtggttgc tgattagctt gagcaagcat tgactgtgat gcttgaacta atatattcat
     9841 tttagataag ttaaccattt ctgaagcaac atctgtatct cttattcttg attcagctgc
     9901 tgtaacattt tctatagtat tatttaagtt gttttgagtt gattctagtc tattttgttg
     9961 tgcccctaat tttgctcttg atgtatttaa ttctttaaga gctacatcta aactagatac
    10021 catttgtttg gctgcatctg ttccagaagc tccatctaca tttaagcttg ctattgtaga
    10081 acttgttata cctgccttac tcattatgct tgaagtattt actagcgcaa cttttatttc
    10141 attgttgttg ttagttgttc ctgcaacatt tgttccaaaa ttagctccaa cttgtaatct
    10201 tatttctgta gaacttccat caagtaattt cttaccatta aactcaattg agcttgatat
    10261 tctttcaact tcatccttta attgtaacaa ctcatcagct atcttttgtc tctcttcagc
    10321 agtattagtt tcatttgaag attgtactga taaagttctc attctttgta gtatattacc
    10381 agtttcttct aatgcacctt ctgctgtttg tacaacagat ataccatctt gaacgtttct
    10441 tcctgcttgg tctaaacctt ttatttgagc tctcattttc tctgatatag caagccccgc
    10501 agcatcgtcc gcagctctct taattctaac accagaagac agcttctcca tagacttact
    10561 ttgtgcactt acgtttcttc ccatttggtt atttgctatc aaagcactta catttgtatt
    10621 aactctcatt attttaccct ccttagtata gttgacatcc ttgtcatttc tactcttaac
    10681 ttttttatta attcttcatt tatataatcg gacaagacta tatataactt tatacttttt
    10741 tattaaaaat atttactttt tctctattta agctataata cattgtgttc acttgtttag
    10801 taagcttata ctcatgtaat tcttttttta cttctaataa ttgtccttgt aaaatatctc
    10861 ttattctact atcaatttca attaattcca ttttattata aagctgtata aaatcatttc
    10921 tatcaatttc acttacactg tttataatct cttgtctttc atctaatttg ctacttatct
    10981 ttatatattc ttccttttca atcaaattta taatttgtaa agagatatct ttatataaat
    11041 ttaatttttc ttttaattta tccataatta attaccttgt gcttgtgaga aataattcat
    11101 ttgggaagaa tactgattca acaaagattc taaagctgaa tatttagcat aaagtctttt
    11161 ctctttatca ttcatttttt caactaacct acttatattt ctttcttgct ctgcaatttg
    11221 ttttgaataa acattttcac tagcagaagc agttttttct atacctgctt ttttaggaag
    11281 tttagaagtt gaacttccag aatattcata tacaatatct tttagctttt caaacattcc
    11341 cttatcatca taattcgaag aatagcctgc tagtgatttg tataccttat cactatcatc
    11401 tataagagcc tttgaaaaag tatctgcatt aaacgatatt tgaccctttt tattgtaatt
    11461 ttcgctttca ttgataccta gttttcttaa tgcaataata ttttttccat ctccaaaaaa
    11521 tgcagattga atatcttcaa caaaacctct tagctcatca tcatttctaa gtataccttc
    11581 tttagctttc ttttcccatt tttctatttc ttcagttgtc atatcttctt tttgggcatc
    11641 tgtaagaggg ggataatcct ttggtttttt agtagtaact aaaccatata ccttgtccat
    11701 aagtttatta taatcatcca caaatgactt catattttta acagcttctt cagtatcagt
    11761 tacagaagtt aactttgcag ttccttttga atttacatta tagtcaatat tatctatatt
    11821 aaacgaattc tgttctttac ttatatgagt tactcttcca tgttcatcag ttacatcagc
    11881 ttctagattt tttccatata cacttgccaa tttgtttcca ccagcatcac ttacaaatgt
    11941 gataccatta tcattagcaa tttgctgagc taaattgtca tttccaatta ctcttaaatt
    12001 aatatctgat gatgaaccag tctgttttct tgaaataatc aattcacctg tcatctcact
    12061 ataagaagcc tttatatcat tatctgcaca aagcgagttt atcttactta caatagtgct
    12121 tgtagtatct tgttcagaga ttggtatatt tacatctcct atagttaaag aactagctcc
    12181 actagcattt ggaggaaact gttgaacaat aggttcactt gaatttaatt tgatagtaaa
    12241 ttttgctggt tcagccattt gagaaactgc aaattgataa tttatttttt ctgcaccagc
    12301 tgagcctttt gctactataa tactttcatc agaactagtt atttttgtag aagagtatgc
    12361 tttttgactt actatactat taggagaatc tacacttaga tatttatcat aaagatcttt
    12421 tgattcttgt ataatatttc tatatatttc ttgcttccat ttaacgattt gttgctcttg
    12481 ttttgcttta tcaacttttt ccttatctct aatcatacta gcttctatta tgccttccat
    12541 atcaaaattt cctgaaagac ctgtaactct tataggactt atacttgaca tccctctatc
    12601 cctccatcca attatatttt tattttattg taaacttcat gccatgtatc tctaatttgt
    12661 tttactattg gcaatatatc atcaattatt ttaatatctt tattttcatc agctattgct
    12721 aattcagatt ttataaattc ataaacttta tacatatcct ttgcccattg ccctgcatct
    12781 tggtctaatg tagtcattag ttctgtaaat atattttgaa ctcttacaag ttcattatgt
    12841 gcttttaaat aatcgttatt taaaagagca tctttggata tgttagtata tttgactgta
    12901 gcatctacaa gcatcaatag taattcttct tttgataact tacctaattc agctaaattt
    12961 aattccatta tttataccta cttctttcct ctttactttt tttatcagct tcatgccatg
    13021 tatctttaat ttgttctatt acaggcaaca cattatctat tatctgaatg tcttttctaa
    13081 tattagctct tgaaagttca tatcttacaa aatcataaac tttatacata tcctttgccc
    13141 attgccctgc attttggtct aatgtaacca ttagttctgt aaatatatcc tgaactctta
    13201 caagctctct atgtgctctc tgcgtatttt tatctaagat agctgctctg gctattttgg
    13261 tatattttac tgctccatca acaagcataa gcaataattg ttcctttgat gccataaaaa
    13321 cagcattttg tttataagaa ttataaggat tttctccata catatattac ttcctccaag
    13381 cttattattt taatgacttt aaaatattta tatttttatt tgtagcttta atattctcac
    13441 tctttacttt ttcgtatatt tcttttctta atatgcttat attgttaggt gctgatatag
    13501 ctagtttcac attgtttcca tctaccccta caatttttac ttctatgttt tctcctataa
    13561 gtactgcttc atcttttttt cttgaaatta ctagcatcca ctatcacctc tcaataatgg
    13621 atgccttatt gaatatttat catcttgtaa aattatttgc attccaaaat tagtttttat
    13681 atttatcact ataggagctt tcatgtttac agttgattct tcaaacgtct gaccaagagt
    13741 aattatatta aatagcataa catcctttgg agattttact tctagttttt ctatggtttc
    13801 ttgatttaaa actatttcat attcattaat aatctcaaat ggcgatacaa caacaaatcc
    13861 tataccttct tcttcagtag aattaaattc ttttaaagaa tcattatctt ctatatctaa
    13921 aagctcatat tcttttaaat tttcaaaccc taatatacct ttttttaatg taaccttcat
    13981 catatctgcg ttatttttac tatatataat ctaatataga tacactaaat aactttcctc
    14041 ctgtttgtag tgaagcttga taagttaact ctgctgcctt taattgaaca agcttttcac
    14101 catagtctat ttcttcattt tgtgacagaa tctcctttaa ctgtatcaaa ctatcatcat
    14161 ttttttcctt catagcctct actgtatttg cttttgtccc atatatagac ctaacattta
    14221 ctgtattttc tattagttgt tccatttcac ctaaacaagt tgttgtgagc tcttttatag
    14281 catcagcatc tggatttgct aatttctttg atatattatt taactcttca aacgggtcag
    14341 tctttgttgg gtcaaatcca aggctactca atttaacatt atagtctaca cttattcctt
    14401 gagctacgtc catacttaaa gaatctttta atttattaag atctatacta gaattaaact
    14461 ttaagcttac tgagccatca gcattatcta cacactcaac aggtggtata cctgtatttg
    14521 aacctgaaaa tatgtactta ccttcatgtg ttgcatttaa agcatttgca atttctttta
    14581 ttttttcatt ggtttcatta ctaagggtct ttagttcgtc atctgaatat gcaccatcac
    14641 cacttgccac caaagttttc tttatgtccg ataataattt cccaagctga tctaatgcag
    14701 tatcagtagt ttctaaccaa tttcctgtat ctttacaatt ctcaacataa atctccatag
    14761 tttttatttc actttttgag tttaatattt ttattgcttt atatggatta tccgatagct
    14821 tgtttatttc tttggttgta ttaacttgtc tattaacttt gtccaatctt tgtagattat
    14881 cttgcaggga gtttagataa ctgctagaca tcatacctgt actaactctc attataaatt
    14941 cctccttcta ttaaattatt ccatttatta caacatccaa cagttgcaca gctgttgaca
    15001 taacttttgc actagcttga taggaacgtt gtatttttat aagctctgtc atttcttcat
    15061 ccaaagatac acctgaaaca ctcattcttg aattatcaat attatttata attttacgtt
    15121 gattcacttc atcttgattc acttttttag aatctaaacc tactaattct gcaaaggctt
    15181 tatagtggtc acttattgta cttacttttc caccagccac tccaatatct attttttcat
    15241 cctttaactt agcaattttt gaagcctcat cagcagtaag ctttaattca tttgggttac
    15301 tctttatagc tggatttacc tttatgatat cttcaccatc tacatttgaa gttacaaaaa
    15361 aatctcttac tgttattcca gcagacgtat tatagacatc atttatctgt ttagctaatc
    15421 catcagagac gtcttttaaa tcgcttttat atgtatcaat cttaccatgc atttcaaata
    15481 atccttgtaa ggttcctgat acacctgttt tccaagttcc atctgcattt tttaattcat
    15541 taagttctaa tttaccatct gcaagcatat ttttaacatc gctattttca aaatctagct
    15601 taaagctcaa gttgtctaat attctatctc tttcatcaag caaatcattt ggcatagagc
    15661 cagaaccaga gataatatca atagatttat tcaaatcttc aagacttttt aacatttcat
    15721 tgatttgttt tatctcagta tctatattag aatctatatc tccttttaac ttgtcaagct
    15781 tcttataact ttcgcttatt aaggttgcta aattagatgc attttctact actagacttt
    15841 ttttactgtc actatttata tcctttgata acgactccca tcctttaaaa aaatcatcta
    15901 tacctgcaga tattccatag tctgatggtt catttagtat agtttctata gtattataat
    15961 aatcatattt tgaaactgtc ttaccatact tatgagcttc tgctctaaat tggtaatcat
    16021 aaaaactatt tctagctctt gttacatctg ttacatgtac tccagtacct aattgtcctc
    16081 ttccaattga attactcaaa ctaggcatat acatagggct atttgtagca aactcagtcc
    16141 tttgcctaga ataaccttct gtgtctaggt tacttatatt atgagaaatt gtctgtattg
    16201 ctttttgatg tgagttcatt cctgaattag ctgtatttaa aatggaaaat aatcctgcca
    16261 tatttctcct cctaatattt attttctaat atttccataa gaattataag ttgttgtttg
    16321 cttatcaact ggttttatga aattaatcat tttttttgta aaaataagtt ctttctttat
    16381 cagagaatca tttgattctt gttgattttt agctaaagca agaatgtgtt ttaggtctgt
    16441 atatatttct tttaaatatc catctttact attttcaaca acctcactca ttgaaatatc
    16501 atcaccaaaa agctgtcttc tctttatttc tatggatgca agttccctac taatatcttc
    16561 tagtttagga tttaacatat ttaaattttc taagtctttt tcaactatat aatcaaattg
    16621 ttcttttaat aattccatca tcttactaat tatatttttt tcttcataca ttataacttt
    16681 taagtctgta atcatgactt tatcctcgca tatcctctat catttttttt gcaatttctt
    16741 ttggttttat tttgtaagtt ccattttcta atgcattttt tatttcatca acttttttat
    16801 tcctaatact ctcatcactc atatttgata cacttgccat ttcctttgca aacttagata
    16861 gttctatctt atctgtttta ttaatttttt taacatcttc cacttttttt acaccagatg
    16921 tattctttaa ataattattc actacaatat ttgaacttac atttttaata tccatagttt
    16981 cacctccaaa attacatact tataatttaa ttatcgctca gaaaataaaa acatttatat
    17041 aattcacaca tattttaaac tttttataaa aatatcccct tagctaaact catacagagt
    17101 agcctaaagg gatactattt aaaatgctaa tttatatctg aatcttctat tttagataat
    17161 cttacagata acttctcatt tgtaataata ctttctccat aagcaaacaa tctatcattt
    17221 gcatatatat ctaatttttc atccacattc ttatttagtt ttattataga gccttctttt
    17281 aaatctaata tattttttat tgtttctgtt gtatttccta tagaaacagc tacatttact
    17341 tttgcattaa aaacattttg attttcttct ataataccag attgttcttc tattagttgc
    17401 ccaaaattca attcatatat atcctctttt ttactattca taaaactttt ctccttgtat
    17461 aatcttatct atacatatct atcaacaaac cttgtatcat atcaacagta gaaacaatgt
    17521 ctatgttttc tttttcttca ctaattaact tactagtcat atcctcaagt ttactgtcaa
    17581 caatctttac attcttataa tactgatgtt cccaaaaact actattctta tcaagtgaat
    17641 acatattatc tacaacagat tttaggtatt cttttactgc atttttatat atactaatat
    17701 cgccatagtt ttgagtaagt acaagccttt ctcctgcctt ttttacactc tctaaatttc
    17761 gttttatatc ttctagtgaa gaagattgtt ttaaagagtt taatctatca gaaaatttat
    17821 tattttcttt tagttttttg acttctatat ttatacttgg acttattttt agcttatcta
    17881 tatttatacc cataataaag cctaccctct ataatatttc ataaccttag gaatataatt
    17941 ttgtgtttct tttggcattt tatacaggtc agaaattgat tccactcctc tactcttcat
    18001 ccttccagga ccaccattat atgccatcaa ccccatttct atacttcctc caaacatatc
    18061 aatatattcc ttaatatgtc tagttccagc atctatattc tgctctatat taaaagggtc
    18121 tgtaacaccc aaatctctac aattttctgg cataagttgc ataagaccct tagcaccagc
    18181 agaagaaaca gtatttggat taaagtccga ttcaacttta attattgctt ttataagatt
    18241 cgcatctaca ttatattttt ttgatgcttg ttctacagca ttttcaattc tcaactttac
    18301 atttttatct tttgtatcaa catctataaa tctactagtg gtattattta ctacattctc
    18361 aagttcatta aacaaattct cagaattaga cgatatatta tagccattag aagtatgttg
    18421 attattttgt gataatgctt ttaacaaggt catcataatc atatcaaatc cagatgaatt
    18481 attatatgta caattacatt gatttgtatt attactacca gataattgag tcaatgctaa
    18541 tatattaatt agtgaactta tctcattcaa tataaaaccc cccttctaaa ttttataaat
    18601 ttcaaagtta cactgataaa acagatttcc accaagattc attttctatg taccacttaa
    18661 cagtttcttt tataccaaaa tcaaaatcat attttgcctt ccaacctaac tcattctgta
    18721 ttttagatgc atcaatagca tatcttaagt catgaccagg tctatcattt acataggata
    18781 ttaaatcctc agatttacct aaaagattta atataatttt tactactttt agattacttc
    18841 tttcattatg acctcctata ttgtaaatct cccctatatt ccctttatgt attattaagt
    18901 ctatagcagt acaatggtca tatacatgta accagtctct tacattttcc ccattaccat
    18961 atactggtaa ttgtttattt tccaaagcat ttaaaatcat aagaggtatt aatttttcag
    19021 gaaaatgata tggtccataa ttgttggaac atctagatat agttgtcaac aatccatatg
    19081 ttctataata agaacttacc aataaatctg ctgatgcttt agaggctgaa tatggagagc
    19141 ttggatttat aggtgattgt tctgtaaaga ataaatcctt tctatcaatt ggcaaatcac
    19201 catacacttc atctgtagaa atctgatgat accttttaac cccatatttt atagatgcat
    19261 ctaaaagtac ttgtgttcct ataatgtttg tttttataaa taaatcagga ttctcgactg
    19321 acctatctac atgagattca gcggcaaaat taacaactat atcaaacctt tcttcttcaa
    19381 atagcttata catctgttcc ctatttgaaa tatctcctct aacaaattta taattggaat
    19441 tgtcttgtat ttctattaat gtctctaaat taccagcata agtcaacaag tctagattaa
    19501 caataaaata atctacatac ttattaagca tataatgtac aaaattgctc ccaataaatc
    19561 ctgctcctcc agttatcagt atctttttca tattttttct cctaaatgtt aattctaata
    19621 aatttttatt aacgtcttat acagatatct ttaaaacttt tttgtttttt atccttttct
    19681 gataaaaata tttcttcaat tccttttaaa ggccactcaa tagatatttg cttatcattc
    19741 catataaaac cattctcaaa ttctggataa taatagtttg tacatttgta attaaatact
    19801 gctttttctg ataaaactaa aaatccatgt gcaaaccctt ctggaatata aaattgtttt
    19861 ttattcttat cagacagtat tacaccttcc cattctccaa atgtttttga atcttttctt
    19921 aaatcaactg caacatcgaa aacttctccc tctgtaactc tgactaattt tccttgagga
    19981 tatctaacct gaaaatgcat tcctcttaaa accccttttc ttgaactaga ctcattatct
    20041 tgaataaagc acatatcaag accttgctct ataaaatccc ttttattata gctttccata
    20101 aagtatcctc tttcatcaca aaatacactt ggttcgacta tatacaatcc ttctatttta
    20161 gtctttatga cattaatttt tcccattata acaaactccc ttaataaaat ttataataaa
    20221 ttttatctac catattacat atcttcacta gctaacttta atagatattg accatattca
    20281 gtttttatca gtggcttggc tagttccatt aattgtgctc tacttatata tcctttttta
    20341 tatgcaatct cttctaaaca tgctatatat aatccctgtc ttgtttgaac cacttcaaca
    20401 tagttagaag catccaatag acctttatgt gtaccagtat caagccaagc catacctctt
    20461 cctagaagtt ctacttttaa ttttcctcgt tttagatact cactgtttat agaggttatt
    20521 tctaattcac ctctatctga tggttcaact agctttgcta tctctacaac atcattatca
    20581 taaaaataca atcctggtat agcataatta gatttaggaa ctgtaggttt ttcttctatt
    20641 gacaatacat taaaatcctt atcaaattct acaactccaa acgaatttgg attacttaca
    20701 tgatacccaa atatagttgc tccttttctt ttaactgctt tctccaatct ttcactaaat
    20761 ccatatccat aaaatatatt atcccctaat acaagagaca ctctgtcatt tcctataaat
    20821 tcttctccta ttataaatgc ttctgcaatt ccatttgctt tctcctgaat tgcatatgtt
    20881 attttaagac ctaatgaact tccatctcca aataattcct caaatgcttt tatatctctt
    20941 ggtgtagata ttattaatat atcttgtatc cctgccaaca taagaaccga catagggtaa
    21001 tatatcattg gtttatcata tattggaagt gtttgttttg atatacattt tgttatagga
    21061 tatagtcttg taccagaccc tcctgctaaa ataattcctt tcatcttttt ccatccttaa
    21121 atttgttttt gtcatattat gattatcgga taaataaaag aaatatttat attttttata
    21181 atatcttatt ttcttttcta aaaataatac atttactctt cattcatatc atatctttct
    21241 aaatattctt caatggattc ttcccaattt ctaaaagaat ttaaaccaat taacttcaac
    21301 ataaaattat ccaatacaga atataatggt ctttgagcct tacatttaaa ttcgttactc
    21361 ttaatcggat ttacttttat gtctatattt tttaattcaa atatcttttt ggcaaaatca
    21421 taccaactac actcaccttc acatgtagca tgataaactc cataatgttc tgtttccatt
    21481 attcttatta taatttttgt taaatctact gtgcttgttg gagttccaaa ctggtcatta
    21541 actacattaa cctctttatt ttctaaagaa agttttatca tagtctttac aaaattattc
    21601 ccattaccat ataaccatgc tgttctcaat ataaagtact tatgtgaaaa ttgttcaaca
    21661 aacttttctc ccataagttt actttttcca tacacagaat tgggttcagt tttactgtct
    21721 tccctatatg gatatttaga aaaaccattg aatacataat cagttgatat gtgaataagt
    21781 ttagcattaa ctttttctga tgctattgct agatttctta ctcctaaggc atttacttta
    21841 tatgcagttt ctatattatc ttcacatgca tcaacctttg tatatgctgc acagtttaca
    21901 actacatcag gtttattatt caaaataaaa ttatctacat tagtctgatt atatatatca
    21961 aaatcattcc ttgttgtagc taatatagca tatttgggtt gactcatact ttgattgatt
    22021 gcttctaatt ggttaaccaa ttcttttcct aattgaccat tagaccctgt tattaatatt
    22081 ttcattaatt atgttatcct ccaataaatg ttcaatttta cttaataatt aattataaca
    22141 cagcttgcat tttaacttag gttgaactta ttatttctta tataaataaa aaagagatgt
    22201 aatattaaat gattatcatc atattattac atctctttaa atctaaatat ataaaatata
    22261 ttgtaattac caattaatta ttagaaactt atctaaaata tttaactcca gattcaaata
    22321 tcttttggtc tttttcacct ataatgttct taaatacttc ctcaccaatt ctctcagaat
    22381 gtcccatctt accaaacact ctaccatcag cactagttat accttcaaca gcatagaatg
    22441 aaccatttgg attaaactca atattataag tagcttcatt attaaaatca acatattgag
    22501 tcgctacttg tccattagca attaattctc tcataacatc ttcactagca acaaatttac
    22561 cttctccatg agaaattgct atattatgaa tatctccaac attagtttga gctaaccaag
    22621 gagatttatt agatgaaatt ctagttctag caatcttagc ttgatgacga ccaatattat
    22681 tgtaagtaag tgttggagca ctttcacttg gaactcttat ttctccatat ggaactaatc
    22741 ctaatttaat taatacttgg aatccattac agatacctaa cattagtcca tccttttgag
    22801 ttagaaattc atttatggct tcttgaactc tcggatttct aaatacagta gctataaatt
    22861 taccagaacc atctggctca tcaccagcac taaatccacc tggtaacatt attatttgag
    22921 aagattttat ttcatttact attgtatcta tagaatcttc tatatcttta taagttaaat
    22981 tcttaaatac tcttatgttt gcattagctc ctgctctttc aaatgctctt gcagaatcat
    23041 attcacagtt tgttccaggg aatgctggta tgaatactct tggagtagct atacttagtg
    23101 atgatttttt agcttctcct tgtgatatat aacttatagt ctctatcttt gattttactt
    23161 cttctgcttt agttgggaat ataggctcta aagtactaca atgtgattta tataattcat
    23221 ctaatgcaag ttcttcacct ttgataaata tactagcttc tttaactgta cttccaagca
    23281 caacataatt gtatccttct aacatactta aatcatcatt tgctaattca agtactatat
    23341 ttccataact agcctcaaat aatttatcat ctttaaatgc tttttcagtt tcttcactaa
    23401 acttaaatcc tattttatta ccaaaagcca ttttacttat tgcttcacct acacctgcaa
    23461 atcctaatgc atatgttgat aaaattttat ttccatgtat aagctctctt actttgtcta
    23521 agtttctctt taattcatca aaatctataa catcattttc tgccttgttt acgcataaca
    23581 ttactacttt tgaatctatt tttttaaatt ctggagatac tactttcttc gcatctactg
    23641 tatcaacagc aaatgaaact aatgttggtg gaacttcaat atctttaaat gtaccagaca
    23701 tactgtcttt tccacctata gcaggtatct caaatttact ttgtgcataa aatgctccta
    23761 aaagtgctga gaatggttta ccccattttt ctggattgtt accaagtttc tcaaaatact
    23821 cttggaatgt aagtcttgtt gtactgtaat ttcctccaat ggcaaccaat ttacacacag
    23881 attctactac tgcatacaaa gcaccatgga atggactcca ttttcctacc tttggattgt
    23941 atccataagt cattattgta gaagtattag tttctcctcc aagcactggt atcttagcaa
    24001 ccataccttg cgttggagta gcttgatact ttcctccaaa tggcataagt actgtattac
    24061 caccaattgt attatcaaac atctctacta atcctttttg agagcaaaca ttaaggtcag
    24121 acaatacctt tataaatttg tctttcatac ttgcacactt aacatctttt actatttcat
    24181 tagagctaaa gaatgtattt tcttcatcta ctttagtaac acggacatca gtagtttgtt
    24241 ttactccatt tgtgtccaaa aactctctat ttatatctac aattgctttt ccattccaga
    24301 acattcttaa atatcctgta tctgttactg tagctacatg tgtagcttct aagttttctt
    24361 ctactgccaa ttgtataaat ttatctttat tttctttttt tatagctact gccatacgct
    24421 cttgtgactc tgaaattgca agctcagttc catcaagtcc atcgtatttt ttaggcacta
    24481 agtctaagtt tatatctaaa ctatcagcaa tctctcctat agcaacacaa actccacctg
    24541 ctccaaaatc attacatctt tttatcattt gagcaacttc tttatttcta aagaatcttt
    24601 gtatctttct ttcgttagga gcatctcctt tttgaacttc tgcactacaa gttaagattg
    24661 attcttcact atgctcttta gatgaaccag ttgctcctcc acagccatct cttcctgttt
    24721 ttcctccaag aagaactatt acatctcctg cttcaggtct ttctcttact acattttcct
    24781 ttggtgctgc tgctataact gcacctattt ccattctctt tgcaacaaag ttttcatcat
    24841 atacttctgc aacttgtcca gttgtaagac ctatttggtt accatatgaa ctatatccat
    24901 gagcagcttc tgtcgttatt tttctttgca taagttttcc aggtaaagtg tcctctaaag
    24961 ttgttcttgg gtcagcactt cctgtaacac gcattgcttg ataaacataa cttcttcctg
    25021 ataacgggtc tcttatagct ccacctaaac aagtagctgc tccaccaaat ggttctattt
    25081 cagttggatg gttgtgagtt tcatttttaa acattactaa atacttttcc atcttaccat
    25141 caacttcaac atctacattt atactacaag catttatttc ttcactttca tctaggtcat
    25201 ttaatttccc atttttcttt aattctttta ctgcaacagt agctatatcc attaaacata
    25261 tatctttatg tctgttcaca tatacattgt ctcttgagtt aagatacatt tgatatgctt
    25321 cttttactat atcattaaac ttaccatctt ctatctttac attttctatt tcagtcataa
    25381 aagtagtatg tctacaatga tcagaccaat aagtatctag aacttttatt tctgttatag
    25441 ttgggtttct cttttctgta tccttaaagt atttttgaac gtgttgtaag tctcctaaag
    25501 tcattgctaa tcctttttca cttacaaatg ttcttaatcc atcttcatct aaatctataa
    25561 atccatctaa tacttcaact gttgttggta tttcagtttc catttttaat gtttctggtt
    25621 tttctaaaga tgcttctcta ctatctactg gatttataca gtaatctttt attttagaaa
    25681 actcttcgtc agttatttta cctgataata tataaacttt tgctgtatta atagcaggtc
    25741 ttatcccttg atttacaatt tgtacacatt gagctgccca atcacctctt tggtcatatt
    25801 gaccaggaag aaattctatt gcaaatactc tttcatcatt taattttggt atttcttcat
    25861 aatatactac atctagattt ggttcagaga atattgtctt tgctgcattt tcataaactt
    25921 cttctgatat tccttctaca tcatatctat ttaatattct taaattttct atattatcta
    25981 tgtgtaaact ttccaccaag tcttttttta aagctttagc ctcaaggtca aaaccttctc
    26041 ttttttcaac tagtactctt ctaaccattg agtctttatt ttcagtattc aacatacctt
    26101 atcctccatt ttagaaaaat tatcttccta tgtctgttct atagtgcatt ccttcaaagt
    26161 ttatcttctt tatattttca taaacttttt ctgctgcatc tttaactgta gctgactttg
    26221 cagttatacc aattactctt cctccattgg taactaaatt accatcaagc atcttagtgc
    26281 cgctatggaa tactactata tcatcatcca agttttcaag accagttata attttccctt
    26341 tttcataact gtctggatat cctcctgatg taagcataac acatacagct tcatcatcac
    26401 tataatttat ttctatatct tttaacttat tatctagtat agattccatt atctcatgta
    26461 aatctgtttc taatctaaat aaaacagatt gtgtctcagg atccccaaat cttacattat
    26521 attcaagtac tttctctcca tcttcagtta tcataagacc aacaaataaa atacctttaa
    26581 aatcaagtcc atcttttttg aaaccttcta aagttttttc taatactgtc tcttttactc
    26641 tatttgctaa ttcttctgta tatatttcac taggtgaaaa agttcccatt cccccagtgt
    26701 ttggcccttg ttcatagttg tttactttct tatggtcctt cgcacttgcc ataggaacta
    26761 ttgtatcatt atctacaaat gctagaattg aagtttctat tccttttaaa aactcttcaa
    26821 ttactatttt atctccagca gctccaaact tcttatctga catcatttct tttaaagttg
    26881 ctatagcatc ttctctattt tcaggtatta caacaccttt tccagcagcc aatccgtccg
    26941 cttttattac tactggatat ccaaagttat ctatttctga tatagcttct tctaagtttg
    27001 tatattcttt atattttgca gtaggtatgt tatgttttat cataaattct tttgaaaaag
    27061 ctttgcttcc ttcaagttga gcacattttt tattaggtcc aaatactctt aatccttctt
    27121 tttcaaactc atctactatt cccataacta gtggtacttc tggtcctact attgtcagac
    27181 ctatttcatt ttctttggca aattttaaaa gttcttctat attagtatca cctatattta
    27241 cgcattctgc tacatttgca attcctgcat tacctggtgc acaataaatt ttttctacat
    27301 tcttttcttt gcttagtttc cagcatatag catgctctct accgccaccg cctacaacca
    27361 atatcttcat aatatcctcc ttagtgtttg aagtgtctta ttcctgtaaa taccataacc
    27421 atatcatact tatcacaagc ttctattgaa tctttatctt ttatagaacc acctggttga
    27481 actactgctg atattccata ttcatgagct aatgttacac aatcatcaaa tgggaaaaac
    27541 gcatctgacg ctaaaacagc tcctgtaaaa tctttatctt tgttattttc aagagcattt
    27601 tttaatgccc aaattcttga ggtttgtcca caacctaaag ctagagtttg tccatttttt
    27661 actattgcaa ttgcatttga tttcatattt tttactactc tcataccaaa ttccatgtcc
    27721 ttaagctgtg catctgttgg ttttgccttt gttacatttt cacttttttc tgctaatata
    27781 ttatttctat cttgtatcaa taattttcca tctaaatatt tcatttcata tggttgtaaa
    27841 ctattttcta ttttagctaa ttttaatatt cttagatttt tcttttgctt aagtatctct
    27901 aaagcttctg gttcaaaatc atatgccact actatttcta agaatatttc atttaatttc
    27961 tctgctgtag ctttgtctat agtagaagtt attcctacta ttccaccaaa tattgaaacc
    28021 ttatccgctt cataacattt tgtatatgct tcaaaggcat cttttcctag acctacccca
    28081 catgaattag catgttttat agctactgaa actacttctt cgctatcttt aaattctctc
    28141 attacttcta aacatccatg taagtcattt aagttgttaa aagaaagttc tttaccgcct
    28201 aattgctcat aatttaagat tggatttttt gcatttgatt gtgaatataa gaaaccattt
    28261 tgatgtggat tttctccata tcttaaagtt tgttcttttt ggaaagttaa atttaatatg
    28321 tcagggtacg tatctccaac ttctccagca aaataactag atattaacgc gtcatatctt
    28381 cctgtagttg agaaagcttt atatgataat ttttttctgt cttctaatgt cattgtatct
    28441 gtttttaatt tttctattat agaatcataa tcttttatat ctactactac tattacatct
    28501 ttatagtttt tggcagcaga acgtatcata gatggaccac caatatctat attttcgatt
    28561 atttcatcat gactttttcc agcttttaaa gttccttcaa aatcatatag atttacagct
    28621 actaaatcta ttgaatgtat tttatgttca tttacagttt ctacatgaga ttctttatct
    28681 cttttataaa gtataccacc atgtatatat ggatttaatg tttttactct tccatctaat
    28741 atttctggaa aatttgtaac atcttcaact tgcattacat taacaccatt ttcttttaat
    28801 gttttaaatg tatttcctgt agatataatt tcataatcta acttatttaa ctctttagca
    28861 aattccacta caccagtctt gtcagttaca cttattaaag ctctcttact catttataaa
    28921 cactctccta ccttgaagtt ttaatttatt ttcacaaaaa agacttatac tttcttttaa
    28981 aatcctatgt tcaacttcta acactctttt tgcaagtgtc tttgcatcat catcttgatt
    29041 taccttaact acatcttgca ttatgattgg tccagtatca gcaccttcat caacaaaatg
    29101 aactgtagca ccagttactt tagctccgta atcgataaca ccttgatgaa ctttttctcc
    29161 atagaatcca gctccacaaa atgaaggaat taatgatgga tgtatgttta tcattttgtt
    29221 tctaaactca ttaaccagct ttgggctgat tatttttaaa taaccagcca aaactactaa
    29281 atctatttta ttttccttaa gaatttctat tatcttgtct tcatcagttt cacaaattgc
    29341 ttttatgtta tgatttttag ctctctctag tccataagca tcttgcttac ttgaaataac
    29401 tactttaacc tgacctttta tttctccaga ctcagtgcca tctataactg cttgtaaatt
    29461 tgttcctcca ccagatatta aaactcctat atttagcata actctacacc ttcatgacta
    29521 tctactactt caccaattat atacgcttta tcttctaata attcactgta ttttttatct
    29581 acataagcag cattgtcatt ttctctatta tttataaagt ttacaacatc atcagcattt
    29641 gctttatcta ctattaaaac tagcccaatg cccatgttaa aacttttatg aagttctctc
    29701 tcattaacag cattaaaacc ttctatcatt ttaaatatag gaggcttctc ccacgatttt
    29761 ttattgatat ctagtcctaa gcctttaggt ataactcttg ttatattttc tataagtcct
    29821 ccaccagtta tgtgagctat ggcttttatt tcatgtttag ctaatacatc taaagccaat
    29881 ttaacatata ttttagttgg tgttaaaagt gcttctccaa ctgtcattcc tagttcttca
    29941 acatattgtt ccattttata attgtaagtt tctaaaaata tctttcttat aaaagaaaat
    30001 ccattactgt gtactccact tgaagatata ccaaccaata catctcctga cttaacatta
    30061 tttccagaaa caatcttttc tttgtctgct attcctacag aaaatcctgc taaatcataa
    30121 tcatcttctc catacattcc aggcatttca gcagtttctc caccaattaa tgcacaacca
    30181 gacattttac aaccgtctgc aattccacca actatttttt caatatgctc aggaactaat
    30241 ttcccaagag ctatatagtc aaggaagaat aatggttttg ctccttgaca aattaaatca
    30301 ttcacacaca ttgcaactaa atctattcca acagtatcat gcttgtccat catttgagct
    30361 atttttaact tagttccaac accatctgta gatgcaagca aaactggctc tttcatacct
    30421 acaaaatctt ttaaactgta taatccgctg aaattaccta aatctccaac tacatttcca
    30481 tcataagttc cttttatttt accttttata aggtctactg ctctatttcc ttcatctata
    30541 tctacacctg attctttata agttaacatg aaagtagttc ctccttatca attttcttaa
    30601 ctgggtattc tccatcaaaa caagctttac agaaagtaga cttgtgttct gttgcatcta
    30661 acattccttc tatatctagg aattttaaac tatcacatcc aatgtattct ctcatttctt
    30721 ctacgttgtt acttgatgca attaatttag agcgatttgg agtatctatt ccatagtaac
    30781 aagaataagc tactggagga gaagttattc taagatgaat ttcttttgct cctgcttctc
    30841 ttaaagattt tactaattgt ttagatgtag ttcctcttac aattgagtcg tctactaaaa
    30901 ttatagactt tccttttatt attgttgaaa gaggatttaa ttttattttt actgctattt
    30961 ctctttcttc ttgtgtaggt tttatgaatg ttcttcctac atatctattt ttaactaagc
    31021 cttcacttat ttttaatcca ctagcatttg cataaccaat agctcctggc caacctgaat
    31081 ctggaactgg aactaccata tcagctttta cagtttcatt ctgagctaat ctttcaccac
    31141 atttaattct aaattcatat gcatttacat tgtctatagt tgcatcattt cttgcaaagt
    31201 atatgtgttc aaatatacaa cttttcttaa ctggtttata attttctgaa taaaaatatg
    31261 attttagttc tccatcttta actactatta tctctcctgg ctctacatct cttataactt
    31321 ctccgccaag tatatctatt gcacaatttt ctgatgcaaa tatatattca tcaccctttt
    31381 taccaagtac aagaggtctg aatccatgtg ggtctctaac tgcaaccaat tcttcttgac
    31441 tcattattac aagggcatat gcacctttta tataatccat tgtcactttt aaactttcta
    31501 ctatatcccc tttgtaatat ctcgcaagta tatataaaat cacttctgta tctgaattag
    31561 tttggaacat atatccatct tcttctagca tatcttttaa ataatttgca ttaactagat
    31621 ttccattatg agctatggct aattgtcttt ttctacaact tccaactaaa ggttgacagt
    31681 tagaaacatg acttcctcca gctgttgaat atctaacatg tccaatccct atatttcctt
    31741 ttaacttaga taattcactt tccttaaaaa catctccaac taatcccata tctttcttgt
    31801 acttaatttc tccatcatct aaaagagcta aaccacagct ttcttgccct ctgtgttgca
    31861 tagaatataa agaataatat agttctttag ttacatcttt attagagtaa atccctaaaa
    31921 ctccacacat acatttgcct cctatttatt attcattctt gaaagtactt ccgtataacc
    31981 ttctacaagg tctccaaggt cttttctaaa tctgtcctta tctaactttt catttgtgtt
    32041 tacatcccaa agtctacaag tgtctggtga tacttcatct gccaatatta tatttccttc
    32101 agaatcctta ccaaattcta ttttaaagtc aactaacttt aggtttaatt ttaagaaaaa
    32161 tgcttttagt aactcgttta tttttaaagt ttcttctctt aagaacttta attcttctct
    32221 agttgctaac ttcattgcta ctgcataatc atcatttaac attgggtcac cataagcatc
    32281 atttttgtag cttatctcaa aaattggctc gtcgaaaact acaccttctt caagaccaac
    32341 tcttttacaa atagaacccg cagcaatatt tcttactatt acttctaatg gtaaaatctc
    32401 tactttttta actaacattt ctctatctga taaactcttt ataaagtgag tatttatatt
    32461 attttctttt aacatttcaa agattattgt tgatatctta ttatttagta taccttttga
    32521 agatatttct gctttttttt ctccattaaa tgctgttgca tcatctttat aataaacaac
    32581 atattcattt tcattatctg tagaatatac ttgttttgct tttccttcat ataataacat
    32641 ctctgctacc cccttaaatt cttgtcgtct tctaacacct tttgtgccat ctcttctcta
    32701 tatgatttca aatcctcttt taattttggg tattttaaag tcattatttg taatgccaat
    32761 aaagctgcgt ttaaacctaa gtctattgta actgttgcaa ctggtattcc ttttggcatt
    32821 tgaactattg ataaaagtga gtctagtcca tccatagttg atgatttaat aggtaatcct
    32881 attacaggaa ttaatgtttg tgaagctatt acacctggta agtgtgctgc ttttcctgct
    32941 gctgctatta ttacatcagt atcatcttct atttctttta ggaatgttga aagttgttct
    33001 ggtgttctat gagctgatag tgctcttgct acaacttcta ttccatattt ttcaagcagt
    33061 tttatccctt cttctaactt tggataatct gattttgaac ccataactac tgctactttc
    33121 attagacttc ccctctccgt ttataagtta ttttacacta taaatcattc gtatgattat
    33181 acgaattata caccttaaat tttatattaa tatacatata ttatcaactc ttttttgaaa
    33241 tttttaaaaa aaaatatttc ttgtattttt ttattcgtca cgtatcgaac tttatttatg
    33301 tttttttatt aaaataaaaa acagattatc tcaaaattga tttttctaat cagattttaa
    33361 acttaatctc ctttttaaaa ttttgtatat tattttttat tacttatttt ttaattaata
    33421 tcttaaattt atagtcttgt tttttaactc tgaatgttac tttcgcttgg ataaaactaa
    33481 tacatagttt attctttatc taagtaatag ctaaaactta aagtgatgat attatataga
    33541 tagatataat tttatttaaa ggagaatctg tatgttagaa gttataaaaa atagacgttc
    33601 aataagagca tatataaata aaagcgtaga agaagagaaa ataactgaaa tactaaaatc
    33661 agctatgcaa gcaccttcat caaaaaatgc tcaaccatgg gaatttatca tagtagatga
    33721 taaggaacta ctaaaaaaac tctcaaaatc acaacataga gcaaagcata tagaatttgc
    33781 accattatgt atagtagtgc ttggaaatag agataaattc ttaaaacctg gtaaatggat
    33841 acaagattta ggtgcttgca cacaaaattt attattagag gttactaacc aaggtctagc
    33901 tgcttgttgg gtaggagtat ttcctaaaaa taaagtggta aataaagtta gacaaacttt
    33961 agacttacca gtaaaattag taccttattc acttatatct attggatata gtgaagaaaa
    34021 aaatgaattt atagaccgat ttgatgaaaa taaaatacat agaaatatat ataaaaatag
    34081 atagtgtttt atttcctcta aactagctgg gacataaaaa tctcctatat atactagtgc
    34141 tatattaggg gatttttctt tgttttgtaa ttgatattaa aaacaagact taagaccttg
    34201 gttttatttt taaaattcat tttatctaac tataaaattt aagatataga aagtacagca
    34261 gttaattatt ttaaatttgt tgagaatatc ttaaaattag ccattttcct taaaatattt
    34321 tagtaaatta atacttccta tcaaaacttc agtaaaaata taccacttaa ttattattaa
    34381 gattaatctt tgtacaaaaa ttcatattgc ctcttaaaag agtacataca aataaatttt
    34441 atctaattgg catttaaata aactatctta caagtattta atagtaactc tcctacaaaa
    34501 ttttattagt atatgagtta actaaatttt aatataaaga gaacataaaa tactgtaaaa
    34561 aagtgttatg gttatttcgt tgaaaagaaa gacatactat taaagcataa attaaaatat
    34621 aggagatgat taaatgagaa tactttcaga aactgaaaaa atatccttag cagcagttat
    34681 aaaaatggaa tctgatggat tacttatgca aagagcaata aatatgttaa tatcagatga
    34741 agacctaaaa agacaatcag aatctagtat attagcaaca gaaggaagaa ttaaagctat
    34801 acaacaattt atagttgaaa atgaaatact aatttctgag gaggtataga taatatgaga
    34861 aatatgatag aaaatctaat taagaacaat acttatatag acgataaagt aataatgtta
    34921 agtatgcttt catctgctaa agcatctgcc aatatgtatc ttaattctgc attaacaagt
    34981 agtacacctg aattaagagc tatatattca gcttcactaa ctcaaatggt cgaaggtcat
    35041 acagcactta cagaattaag tattaataaa ggatggttta aaccatatga taaacctatt
    35101 caacagttaa catgcagtta taaagaatcc caaaatgtta ttgactaaac taaataaaac
    35161 atatttctaa ctatttcact ttaattaaat aataaaaagg ggatattcaa attttttaag
    35221 atttcccctt cttctaaata atctattttt taatataaaa tagattgctt acaatctaaa
    35281 gatataattt tatatagtta ttattaatta ttttagacaa ttctatttat taacattttt
    35341 tactacatat ttttataaac aaagacatca aatccttatg ttccaaaatt aaaaaatcag
    35401 gcttataaaa caccccttct acttcatgtc tcctatggtt caaccatata ctcttccaac
    35461 cagctttttt agctcctaat acatcatttc tataagaatc acccatataa tatgtatttt
    35521 ctctatctaa attcataaca ttctcagcta ctctaaaaat attagtatct ggcttagaaa
    35581 atccaacctc actagatata aaaatattat ttttatcaac ccagttttca atatttaact
    35641 gctttaactt cattctttga tgagttgatg gaccattagt tataattcct agattaatat
    35701 tcctttcttt agaaaaattt aaaacccact caacttctgg tattagtgta atttgtgatt
    35761 gttggtatat atactcatct tgaaaacttt gtgcatcttt ttctgtaatg gaagttccca
    35821 attcctcaaa agctttcatt attctataaa tatgcatttc tttgattgcc atttctccat
    35881 tctcagtcat atgaaatacc tcatcactat attttctact actcatatat aagtcttcaa
    35941 tagaaatatt ctttattgaa gaaaacactt tattatatgc aatataaaaa ggtgttaatt
    36001 gattatataa agtatcatct acatcaaaaa ttaaattaat caaaatatta gtcctcctaa
    36061 atttaatagt ggtaagtata attataacta taaataccta gtataacaat actcaccact
    36121 ataagtccca ttatttattt aaaatccttg tagtaacacc ttttaagtta taacattccg
    36181 cataatcacg taaaggacgt ttaatgtggt ctataacaaa tctttgacca tcaatattat
    36241 tcttacttaa attagcataa agcacttcta tttctttttt gacatcatca ttattaaatg
    36301 tataatgccc tgcaatatct aatatttcat tagataaaac ttcattctta aatatatctt
    36361 cagtagttag atttttttgt tctcctatta cccattttct ccaacgtcca cattcaattg
    36421 cattaacaag aagaacatgt ctgatattag aagttttatc aactaaaccc tctttttcta
    36481 atttacactc aacttctgct aattttaaat atgctctagt ttcttcagta ccatattgag
    36541 gagctacatt tgtagcaata atactagatg gtatatgttc taataaagtt acatcatcta
    36601 agtaatcacc attatgctct tttaaaccaa ctccatattt cttagccatt tgagctaaat
    36661 cataagcatt tctaaaatta aaagttccta cttgttctgt ctttctagtt aaagtaccag
    36721 tttgaccaac tataaatgtt ggcattggca aacctctctt atcaagttca acttttagtc
    36781 tagttataaa tgtttcatac gtctcagttg aagtcagacc tccattggtt tcctctgtac
    36841 caacttcata acctatttct ggtagattga gtctttttct ctcattttca caatattcaa
    36901 ttaattctac agttctttct agtactacat ctagtggaat tacttttcca atttcaaatg
    36961 ggtcttttgt agggtcaatc attaataggt caaaacctgc ttcaatgtcc gctaaataag
    37021 atttttttcc taactccatt gcctcatcaa caggtaaatg gtcatttctc tccttatctc
    37081 tttgccatgg tccaccatgg tctctacata aataatataa accatcaaat ccaactttat
    37141 cggcaatctt cttaatatct tttgtaaaag tttcttgatt ccaaccattt acatagccgc
    37201 cacctagttc atctgcatct acttgatttc tgctcgcaat aaacattaaa ggaaaatcat
    37261 catcttttgc taattcaaaa cttgcttgta acaagtttgg tgacatagga ccaattccta
    37321 aaagagttgc acttttcccc tcttcttgta attctagtaa tctttgtacg acagtcttta
    37381 ttggtaactt tttcatgact atatctcctt ttattctatt tttaagatat tttcaaagat
    37441 gtttttaata aaaatctcct taaaaacatc tttgagttat atgtaaaaca aatcaatttt
    37501 tattgaaaag tatcttttat tttcttaatt aatgcatcag atttcttaat tatgtcagat
    37561 acactcattc ttactacttt ctttccctta aaacgttcca tatttctaat ctcaacatca
    37621 ttagtcaata ttacacaagt tgcttcctca atctctttaa gcgacaattc attatctatc
    37681 ccagagcttc cttgagtttc aacctttatt tctatgccaa actttttggc tgataattca
    37741 agtgattcag ctgacatgta agtatgtgct actccactag gacaagatgt tactgctaca
    37801 attttcatat tcttatctcc tttagactta tatttttata tatttacttt tttatacaaa
    37861 ttcctttctt atttatattt ataattcttc aaatattaat tcaatcgagt catctaaatc
    37921 ctcttcaatt tcttcatctt ctttcacgtc tttttttact aatttcatta atattgcaac
    37981 aactactgct ccagttaata ctgatgcaat ataccccaac ctattgtcaa ctacgggtaa
    38041 tactatcaga cctccccagg gtgcatgatt taaacatcct aataaaaaag ccataatgtt
    38101 tcctataatc gacccaacca taatacaagg tattactcta acagggtctg atgttgcaaa
    38161 tggaatagct ccttctgtaa ttccaacaca tcccattaat agtgaagctt ttcctgaatc
    38221 tctttcttcc ttgctaaatt tttttggaaa caataatgta gctaatccta aaccaattgg
    38281 aggtatacaa attgcaacac caactccccc cattaaatat ggaagtgtgt caacttgagt
    38341 ttgagcaaaa gtagctgcaa ccttatttat aggaccaccc atatctgaag ctatcattcc
    38401 tcctaaaatt cctcctaaag gaattttaga aacaccagac attccattta accaattagt
    38461 catccatgtc ataaaagaag ctataggttc tccaattaaa aatacaatta taccacctgt
    38521 aatcaatgta ccaatcaaag gataaataaa tatcgaacct aaagtcctca ttgtaggagc
    38581 taatttaatt ttctttaatt gtaaaacaac aaatcctgct ataaatcctg ctatgatacc
    38641 acctataaaa cctgcattta cttcttttgc taaatatgca cctatcatac ctggagctaa
    38701 tcctggttta tctgccatac taaatgctat atatccacct aatataggaa tgaataaggc
    38761 caagccggca gctccaattt gattcagttt agccaaccat cctgtttcag gaacagcagc
    38821 ttgtccactt agagttactg ataaagcaaa taatattcct cctgcaacta caaatggaat
    38881 catataagat gttgctgtca taagatgttt ttgcaaaaac tttaaaattc tcattttaat
    38941 cttccccctc tattttttct tttataagat tataaacagt ttgttcatct gaacatctaa
    39001 tttgatttct aaaactttca tgcatcaata aacgtgctaa tttcgataaa atctttaaat
    39061 gaagattatt ttcattgtct tttgttactg atattaaaaa tattaaatcc actttttctc
    39121 cttcttctgt ccattcaata ggagtatcaa gttttcctat agtaatacaa gagtttttaa
    39181 cacccacact ttgactatga ggaagaccta taccatgtcc aatataagtt gacaatgttt
    39241 cttctctctt taaaacatcc tctaaaaatt gatttttatc tcttaaatat ccttcttttg
    39301 atactacatc aactaatgat ataatggctt cttcttttga attagcatgt attttaaatg
    39361 aacattttac attaactaat ttactcatac agttacctcc ttctcatatt tatgaattaa
    39421 accttctgct tcttcctttg tctttgcact ttctaacata gaaataaaat tagtcttata
    39481 aaccatttta gttagattaa ttattgcagg aatttgctct tttttatctt tgcttgctaa
    39541 acaaaatatg attctaacct ttttatctcc aaaactaact gcttctttac atattaatag
    39601 acttatcgaa cttactttga ctaatgaact atcattacca tgtagtaaag caaattcgtt
    39661 atcttttaca gcataaaaac ccacattttt taaaatatct ataatatttt cagcatattg
    39721 tgatgataca aaatcacaat tagcaagtat atttgaacta atttttacag catcttccca
    39781 tttcatatct tcatcaatta tccttatggt atcaattcct attaaatcgc ctaaattaag
    39841 ttgtctagat aatgctctct catcactgta tccaagttct tgacatataa cacttaatac
    39901 tcttttttga gcatcttctt ctaaaaagtc caaccttttt tttattgaca tataattagt
    39961 taagacgttc tttcttttta tacctttttc ctcaaacttc aaataatcct cttcttgtaa
    40021 aaatggattt atctcaataa ttgttttagg caagtctatt actatctttg atgttgttat
    40081 aatgaagtca atactttccc agtctttata atcttgtaac ttatatgatg gaatactgtc
    40141 cactatattt atctggtaat catttattag actatctttt aacatagcaa ttacaccata
    40201 gccaaatcca caaactaaca atatattttt gtagttattc ctctcatttc tatgtatact
    40261 tcctaaaaaa tgtattgcca aaagtattgt ttcattttca ttctttattt gatttaaaac
    40321 tctgttcttt tgaatagaaa ccttagtagc ttcaagtaca tattggtact tatctggtat
    40381 tacatgaagt aattcttcat aaatctgtat gttctgtttg attctctcta tcaatggggc
    40441 aatatgattt agtaatcctt tataaagaat catatcttct ttaaaactaa tctctaatat
    40501 ttctgacata ttgtaaatta aatcttgaac tataatctct gtactaatca gatccatatc
    40561 ctcatttaag cttgcatatt tatttgtaaa aaatacaaaa ctctttaaca gaaaaatttg
    40621 ttccttagtc aacttttttt gaataattgt ttcaaactct tggtcaaact tattttcaaa
    40681 acatggctca cttaatgaaa cagattctaa aggatgtttt tcatctgaat gtatatacca
    40741 tgtaaaaagg aaaatgtttg acacatacca tgtgaaagat tcatcattta aagtccatcc
    40801 catttgcaat aataaattca tagaccaatt atatatatca atcaaagaaa tccccttgaa
    40861 tattttttca atcacattca ctaaatactt ctcaaataca cttaaatcag atttttcttt
    40921 accaaaaaga taagaatatg attttaatat attcaatctt tgcttatata taatgtcttc
    40981 ttctccactt attttaaaag ctttatcata agtaatagtt atttgatttc tttctaattc
    41041 acttgataaa acattaaggt catttttaat tgaacttcta gaaacctgaa attctttact
    41101 caatttttct aagtttagat tctctatttt aaacattgat ataattttca taatagatac
    41161 tcgctcagtt aaggaaaata catattcgtt attatttata catactactt ggaagttatc
    41221 aggaataatt aacacacctt tgttcatctt ttgtattact ggcttgttta atgaacgcaa
    41281 aacaatattt aaattattta tatcatatct aacttttctt tcttctatgc taagttgttt
    41341 gctaatatcc ttgtaacttg tcttattttt tccttttaga aaattcatga tgagtaacat
    41401 tctcccactt atcatatttt ccctccttcc aaaataatta gagttattaa tatagcaaat
    41461 tttacattgt tatgtatgtt ttagtttttt tatctaaaac agtaactatc tttctatatt
    41521 cagaataata caataaagct ttagccacaa cttacaacat ttgaaaatta tttttacaaa
    41581 atctgcaaat acatttttta ttaaatatat aaataaaaaa ggcaagttat gttttaatta
    41641 tataacttgc ctaaattttt tatcttatcc atttaataat cagatatttt taattttatt
    41701 attttcatat gcaaaatctt caaagtcaga aattagtagt ggtttagaaa aaacataacc
    41761 ttgaatatag tcacatccca cattttttaa gaactcaact tgctctaatt cttcaattcc
    41821 ttcagcagtt actttcatac ctagcctttt gctaagttca ataatagact taataactac
    41881 cttaccacgg tctatattat ttttgctgac aaagaacgaa cggtctaact taattatatc
    41941 aactttaaaa tcctttaata ttcctaatga tgaatatcca aatccaaaat cgtctataga
    42001 acatttaaat ccattagaat gaatatcatc tattatatct acagcttctg gattttctaa
    42061 gaaaatagat tcggtaagtt ctaaatctat cagatatgtt ggtacattgt atttattgca
    42121 tataagctta tatttttcta aaaacaattt atcattcaaa tgttgacgtg atacattaac
    42181 agaaattctc aatacttctt taccttcttt tatccattta gaaatcaatt tgcatgtttc
    42241 ttcaagtata tacaaatcta acttacatat aaatccactt ttttcaaaaa gaggaataaa
    42301 atcacttgga taaattatgc ctttttccga atttttccaa cgcacaagag actctgcacc
    42361 actaattttt ccacttttta tatcaatttt aggttgtaaa cacataaaaa attctttatt
    42421 ttcaagggct ttttccatta aatttgaaat ttccttttca gatattaatc gttttctttc
    42481 tatatcacta taaaaagaaa aatcactttg atgagcttta ttaaagcttt ttcttgctgt
    42541 atttgcatgg tcttgtatcg taattaaatc ttcattatga ttttcaattg tgtaaatacc
    42601 tgccgatatt cttaaaaagt atggattttc tctattttta ttaaaataat ttatatcagc
    42661 ttctatttta tataataact ccaaggcttc tagcactggt ctattcttaa taagcaaata
    42721 gaaaaggtct gcatcatgac ggcacacaaa ttcttcattt tcatcaatat ttttatataa
    42781 aacattgtaa agatgtttta gtgtcaaatt tcccttttta ctgccaaaac aatcatttat
    42841 tagcttaaaa tcttttatat ttatagctac catcatgtaa gtactatcag gtgattctaa
    42901 aattaaaggt tttactaaga tactgaattt agttgaattt ataccatttg ttatactatc
    42961 taaatatgca atattctcta tttgatttac atattttctt cttataatat ataaaatcaa
    43021 tgttataatt ccaataatac atgtaattat aaatgtcatt attattgtaa aagagtttaa
    43081 cttattaagt tcaccaagta cagcatcttc aggtactata agaacaaaat gccaatcatt
    43141 taaacctttt gtaattggat aataggttaa taggcattgt acaccttttg atgaagttat
    43201 tgtaatattt cctgatttat catttttaaa atcattaagc atcttttgtg cccaaggttc
    43261 atctccagaa ctcttatatt tatggccaag gtcttttatt aattcagtat ttttccctat
    43321 gaaaagaggt cttccctgac tgttaataat ttcaatagtt ccttctccat taaagttatc
    43381 tatattaaga atctcgtcca tactttcttt attagataca cctatcaaaa tactagacac
    43441 ttcatgatta tcattgaata taggtacact atagaaaaca aaacccattg actctattac
    43501 ttctgagtat tctttacctt caattccttt tttgtaggaa tcagtattct ttaaatcaaa
    43561 cttttttccg cttaaaaatt cagctttacc ctctaaatct attattccta tatcataata
    43621 cttgtaacta gtctttcttt tagataaata ctcatatcga ctatcacctt catattgctt
    43681 aataaaatct gccattattc ttaaatcgga taaaataaat ttgattttag aatcaacatt
    43741 cattacaatc tgtgtagata catcatttaa atagttttta gattgtttac tagatatatt
    43801 gtgtagtttt acagtattaa aaacagtaat tacaaggatt gaaataataa gtgaacataa
    43861 aattatagac agaactaaac tatttttctt ataccttgat aatttcattt ttttcctcca
    43921 aattataaac ctatttttta atgtatattg acacttacaa atttttttac taagaaccct
    43981 tcacactata ttatattata gtattccatt tattaatat
//
LOCUS       DAMJVM010000023        43946 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.023, whole genome shotgun sequence.
ACCESSION   DAMJVM010000023 DAMJVM010000000
VERSION     DAMJVM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43946
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.023"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            70..1251
                     /locus_tag="PC523_001354"
                     /pseudo
     CDS             70..1251
                     /locus_tag="PC523_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            1367..3304
                     /gene="htpG"
                     /locus_tag="PC523_001355"
     CDS             1367..3304
                     /gene="htpG"
                     /locus_tag="PC523_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDF2474891.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKIIVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDTRNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEVVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFHVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKETVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKVTLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            3683..4813
                     /locus_tag="PC523_001356"
     CDS             3683..4813
                     /locus_tag="PC523_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2474892.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKATGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKSSIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPIADVQAIYG
                     IELISENLVKVYNNVNDLEAWEKITLASTIGGMTIGTAGTALSHGMEHPVSGLRDVMH
                     GKGLAALTPVIIESSWDSDTERFGNISKILGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEEIKEMYRKSL"
     gene            5041..6063
                     /gene="splB"
                     /locus_tag="PC523_001357"
     CDS             5041..6063
                     /gene="splB"
                     /locus_tag="PC523_001357"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HDF2474893.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKNIYNKFKDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GKEEKGRFRFVTKYSDVDSLLDAKHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIRSIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFHKEINGVFKNS
                     LIKYII"
     gene            6230..7141
                     /locus_tag="PC523_001358"
     CDS             6230..7141
                     /locus_tag="PC523_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2474894.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLAKASEYLAEHKNRILDVALKFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVTRKTLSNPRTFVGIEMEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VELGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTAD
                     FAIEMYYPTTPEVAYLEHWIVPIPIEQ"
     gene            7156..7572
                     /locus_tag="PC523_001359"
     CDS             7156..7572
                     /locus_tag="PC523_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF2474895.1"
                     /translation="MNWQDTFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVNESNRVEVDLFINTCCDYIK
                     QIYNEVKFFNGGKWLMIQVDSLLVLNDMLELIKFRI"
     gene            complement(7672..8064)
                     /locus_tag="PC523_001360"
     CDS             complement(7672..8064)
                     /locus_tag="PC523_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2474896.1"
                     /translation="MGINSEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESDGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNKCGLNCKECRKTK"
     gene            8205..8600
                     /locus_tag="PC523_001361"
     CDS             8205..8600
                     /locus_tag="PC523_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2474897.1"
                     /translation="MKALEFLNKAGVYYLATADENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            9001..9207
                     /locus_tag="PC523_001362"
     CDS             9001..9207
                     /locus_tag="PC523_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474898.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGVEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
     gene            complement(9513..10256)
                     /locus_tag="PC523_001363"
     CDS             complement(9513..10256)
                     /locus_tag="PC523_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDF2474899.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIKNIPRIINNIIILRGCEANILDTKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNHPKKLINHFHQKGKLLDVDYRNI"
     gene            10422..12989
                     /locus_tag="PC523_001364"
     CDS             10422..12989
                     /locus_tag="PC523_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2474900.1"
                     /translation="MTQRKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFSNINSINEYS
                     EDDSIKDEYILEGIKKENNIFNLVGCDGSLKSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLNIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGILFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESIRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKSFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDEDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYVLQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANEIDKGKGILILTDMGSILTFAKVIHDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEITYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRSKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            13219..13623
                     /locus_tag="PC523_001365"
     CDS             13219..13623
                     /locus_tag="PC523_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2474901.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            13647..14144
                     /locus_tag="PC523_001366"
     CDS             13647..14144
                     /locus_tag="PC523_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2474902.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEIELNHNINRY
                     KGEFI"
     gene            14147..14548
                     /locus_tag="PC523_001367"
     CDS             14147..14548
                     /locus_tag="PC523_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDF2474903.1"
                     /translation="MNKYKVIILCHGTWGKLLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKGVEEAVDKEDVKIIISDLYGGSTSSVAISVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            14566..15054
                     /locus_tag="PC523_001368"
     CDS             14566..15054
                     /locus_tag="PC523_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2474904.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGSEDIKQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            15081..15836
                     /locus_tag="PC523_001369"
     CDS             15081..15836
                     /locus_tag="PC523_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2474905.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLNVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            15836..16699
                     /locus_tag="PC523_001370"
     CDS             15836..16699
                     /locus_tag="PC523_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2474906.1"
                     /translation="MQEIKNKDVKNGQGTTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKKKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIIFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLSVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            16746..17225
                     /locus_tag="PC523_001371"
     CDS             16746..17225
                     /locus_tag="PC523_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HDF2474907.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGVNPDDIC
                     KEAIYGFGKMRGQKYSVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQSVRLEFAQKRAHDDEVCELVFTRK"
     gene            17290..18597
                     /locus_tag="PC523_001372"
     CDS             17290..18597
                     /locus_tag="PC523_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2474908.1"
                     /translation="MDISRRVDNSLNRTIEFLKELIKIDSQQGEPILQCPFGIGPKKS
                     LEKTLDYCASLGFSVKNIDNYIGYAEIGEGKELIGIPMHLDVVPPGDGWSVDPFSGVV
                     IDNIIYGRGAVDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMECIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIILRGGTKENVVPSHC
                     TAKIVNGIILELTTKGVAVHASNPEKGENAISKMVIKILKDNMNFQHREDIELVSKYL
                     CSDYYGNALGINRYDGVFKNTTLNLGILKVNEEKIVCELDIRYGKNIILNNIIDRFKK
                     FFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            18914..19036
                     /locus_tag="PC523_001373"
     CDS             18914..19036
                     /locus_tag="PC523_001373"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HDF2474909.1"
                     /translation="MLSNKIIKKCCKIGYMIREKSIDIYSDSGWEFMVWDENGE"
     gene            19540..20634
                     /locus_tag="PC523_001374"
     CDS             19540..20634
                     /locus_tag="PC523_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2474910.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     LDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTAKPFDEVMIEIREI
                     IKRYYSCFPLIFRMGLLIVNHYDIVDEKKRELLIDEALEIFIRIQETCNDIDICRQAK
                     NMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQIDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISNIFELDTLSPATMLSAYLTQTQINLIH
                     EDNEGAIKSLRKYVDLATSDIYPITIHGDDFFDKLDRWISEIGIGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            20639..20839
                     /locus_tag="PC523_001375"
     CDS             20639..20839
                     /locus_tag="PC523_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HDF2474911.1"
                     /translation="MENLMEYLPLLIPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISIIGPILYFTIGRGEE"
     gene            20839..21756
                     /locus_tag="PC523_001376"
     CDS             20839..21756
                     /locus_tag="PC523_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2474912.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLEPDSGTIDVCGEKVTYGGTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKQEIKIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSGDIKSLLKYSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            21753..22511
                     /locus_tag="PC523_001377"
     CDS             21753..22511
                     /locus_tag="PC523_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2474913.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLVFGIMSPLSAKFMPD
                     LIAHFAPTLKVTTAPTALDSWTQFSGNISGLGASLTLIVFCNILSNEYSKGTLVIMLT
                     KGLSRSSVILSKFSIAVIIMTIGFWLSFLCTYGYTMYFWPTENLNHIIFSAFNLWLIN
                     IMYITILILGCVLFRPAFASVLLVLVATLILSLISIPKQIAPYTPNFILSKNIDLVSG
                     KVAAPEFIIPMIVTIVISIICLLLAVILFKKKAV"
     gene            22987..24060
                     /locus_tag="PC523_001378"
     CDS             22987..24060
                     /locus_tag="PC523_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDF2474914.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDKAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKECEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCIGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKIGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            24130..24393
                     /locus_tag="PC523_001379"
     CDS             24130..24393
                     /locus_tag="PC523_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474915.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            24599..25576
                     /gene="bioB"
                     /locus_tag="PC523_001380"
     CDS             24599..25576
                     /gene="bioB"
                     /locus_tag="PC523_001380"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDF2474916.1"
                     /translation="MEKYIIKLKNKVLGGKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREFFMGRKADLCTIINAKSGKCSEDCKFCAQSSHYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNDILKIYERLRENTNLKLCASLGIIDYEQAKMLK
                     SVGVTTYHHNVETARDNFHNICTTHTYKDRIKTIKEAKEAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMEDYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDEVGFNGGVNSAIVGDYLTTVGNGIENDKKMIIEQGFEI"
     gene            25804..26817
                     /locus_tag="PC523_001381"
     CDS             25804..26817
                     /locus_tag="PC523_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2474917.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANKEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKYLLDTGNKKIIYLSGPLQNEIAIDRLEGYKKALIECNLSYNTDY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDVSVVGFD
                     DIQTSSLISPSLTTVRQPSYDIGYKASEILINCLKGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            26859..27752
                     /gene="rbsK"
                     /locus_tag="PC523_001382"
     CDS             26859..27752
                     /gene="rbsK"
                     /locus_tag="PC523_001382"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDF2474918.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCVGEDSLGEELIRNFRTNKVDYSLVSRNKHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRINVL
                     LNPAPAIKLDEDIIEKVTYLTPNEHEYKIVFDTNEEIEEVLKKYPNKLVITEGKNGAR
                     FYDGNEVKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEEVEKYLNNNK"
     gene            27800..28762
                     /locus_tag="PC523_001383"
     CDS             27800..28762
                     /locus_tag="PC523_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDF2474919.1"
                     /translation="MKVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGASDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            28815..30326
                     /locus_tag="PC523_001384"
     CDS             28815..30326
                     /locus_tag="PC523_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDF2474920.1"
                     /translation="MSNIILKLSNIAKEFPGVKALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFQDKEIENISPDVSKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDVNPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLIKDLKDSGVSIIYISHRMEELFEICDRITIMRDGK
                     TVSTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDVSFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALRIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGNLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            30310..31308
                     /locus_tag="PC523_001385"
     CDS             30310..31308
                     /locus_tag="PC523_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDF2474921.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSAFRDQGSAAVGLVIIFIIMSIAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWLGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYVGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKLAKSK"
     gene            31492..32754
                     /locus_tag="PC523_001386"
     CDS             31492..32754
                     /locus_tag="PC523_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2474922.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGIADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEHNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGINAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNVHKILEETLGRSLEIKGSTAGNDARLMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            32774..33517
                     /locus_tag="PC523_001387"
     CDS             32774..33517
                     /locus_tag="PC523_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDF2474923.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDYNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     VDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            33690..35075
                     /locus_tag="PC523_001388"
     CDS             33690..35075
                     /locus_tag="PC523_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2474924.1"
                     /translation="MSDLNKKFLIEYIDEIQEELRDISDKIWKNPELQYKEYYASNLQ
                     KEYLKKHDFSIDELEGMDTAFVASYGEGEPVIAVLGEYDALDGLSQKVSITKEPILDS
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDSCRVHYVTTNGGKMPNIVPDFAEVWYYIRGIKMEHVRDVFDRIVDIA
                     KGASLMTGTSMEYDIISGVYDYIPNTILTDILSQNMKLIGVQNYNEDDYHFAEKLAET
                     VSIDKRISVSSVLSGNKDITKMNLHGEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSIACSINDVMLDEVIINKAKKELKN
                     TIGSFEYIPII"
     gene            35287..36126
                     /locus_tag="PC523_001389"
     CDS             35287..36126
                     /locus_tag="PC523_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474925.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDRRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFAYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYMNILESRGGK
                     NVQ"
     gene            36116..36559
                     /locus_tag="PC523_001390"
     CDS             36116..36559
                     /locus_tag="PC523_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474926.1"
                     /translation="MYSNTYNNEHFMRARAFIENNEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            36570..37061
                     /locus_tag="PC523_001391"
     CDS             36570..37061
                     /locus_tag="PC523_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474927.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIALGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVFSSFIGYYNNRLRKML
                     FKK"
     gene            complement(37263..37991)
                     /locus_tag="PC523_001392"
     CDS             complement(37263..37991)
                     /locus_tag="PC523_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HDF2474928.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            38116..39204
                     /locus_tag="PC523_001393"
     CDS             38116..39204
                     /locus_tag="PC523_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474929.1"
                     /translation="MNFNFAKATNSRLMGSLGLMINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLKNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYNKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYDKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNIVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSDMNEGIFF
                     TRFNFNNDHVKENVYVINNDMKAIYYLMGNKFFIGTYDENDCNYINEISQSNYSDYIK
                     FEENFFFEQNALYDFAESGSLDFDDFLE"
     gene            complement(39642..40532)
                     /locus_tag="PC523_001394"
     CDS             complement(39642..40532)
                     /locus_tag="PC523_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474930.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQGELRNDPQISN
                     GYTKVPNNQNINNLYPNNINNYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNS
                     NMGTPNNMSNNMNSNMGIPNNMPNNMNSNMGIPNNMPNNMNSNMGIPNNMPNNMNSNM
                     GIPNNMPNNMNSNMGIPNNMPNNMNSNMGIPNNMYGNMNNNMYGNTGMPNNMNSNMGY
                     NMNNTMGIPNFSCNQNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSN
                     MSMQGIPQATNIEEFDEEEM"
     gene            40732..41097
                     /locus_tag="PC523_001395"
     CDS             40732..41097
                     /locus_tag="PC523_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2474931.1"
                     /translation="MREEINDCNCNIIHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            41120..43507
                     /gene="cadA"
                     /locus_tag="PC523_001396"
     CDS             41120..43507
                     /gene="cadA"
                     /locus_tag="PC523_001396"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDF2474932.1"
                     /translation="MEANNLIKKEFILGGLNCAHCAEEINDKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDATIEKIIDIIDSTEPGLDIQINSKENASNKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTINISNNFEEDDVINKIKEIINSTEPGLD
                     IQVWSNDKVKERTTEKSGVANDTNKKELISLIIGVLVYIFGIYQTATGYESQFSNIVF
                     IVAYVIIGGDVVLRAIRNISKGRVFDENFLMALATVGALAIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVKVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFIPPLVIPGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGKFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIKSLKAIGVKEVVMLTGDNEKVAKNIARELELDTVYSNLLPNEKVDRLEELYEGRT
                     EREKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKMIVMILGAGGVATMWEAIFADVGVSLIAVVNAMRAMR"
BASE COUNT        17352 a         4467 c         7901 g        14226 t
ORIGIN      
        1 taagacatat tttagtacgc aaaattctgt gaagcaggaa ccctgtgact ttagtcatgg
       61 gaggttcagg aggctgtaga aagtttctat aagagttcac tttgtgaaaa aactgctctg
      121 tgtgaaaagt atataaacat gcttacatct gaggagtttt taattttctt tgaaaggtac
      181 aatcaactta taaggttaat aaacaagggt atgtataaag aagctataca agggtttaaa
      241 cttataactg agcaatactg tgatttaata gaaccttatg agttacttgc cttactgtat
      301 gacaaggaag aagaatatat aaaatttgat gaatgtttag aagtattgaa tactattgat
      361 aaggaaaact atttattaaa tcaagaaaat aatacactag atatgtataa tagtactaat
      421 atacaaacta aaagtaatgt aaaagtagat gattataaga atgagttttt acataaaagt
      481 agtaataacc ataaaattga tgattataag aatgtaacta aagaaaagag ttcaagcata
      541 aaccagaaaa attcagattc taataagaaa aaaagtatta aaaaaaataa atacctatat
      601 atagcaatag tattaggaat attattaata ggacaaggta tatatagttc ttataagatg
      661 aataatttaa ctgataaaat atctaaaaat aatgaaacta aaaatgaaga gtcaacagat
      721 tctagtttgg ctaagaaaga aaacaatata gaagaaaatg aagatatgaa taagacttct
      781 aatatcgata agagtaataa aaatattaat aaagaggata taaaatctaa aaaaaatact
      841 gtagagaatg aagatgataa agattctgac ttatttacag aggatgaatt gatgtctaag
      901 gcaaatactt taaaatcaga aaagaaaact aaatcttcta tacaactata taagaaagtt
      961 gcagatatag gtgaaaataa aggaaataca tctgaagcaa cttatcaagt tgctattctt
     1021 agtgaaaagt taaaagacta taaaactgct gaagaatatt ataaaatgta tgtggaaaat
     1081 tactcagaaa aagatgctta ttttgatgaa tcttattata acttaggtat gatgtactat
     1141 aacaatggtg atttaaaaag ttctaaattg acattaaaga agttggttaa taaggtgcca
     1201 aatagtatgt ataataattc aaaagtaaaa gaaattttaa aagaagaata gctaaattag
     1261 ataagaaaaa taaattattt tattttaaaa taatatgatt ataatatatt gacataccat
     1321 tttaaaaaat ttaaaatatt aattataaaa tgaggaggga taaaaaatgg agtttgaaaa
     1381 agggagtatt tcaattcata cagaaaatat ttttcctata attaaaaaat ggttatattc
     1441 tgataaagac atatttataa gagagcttat aagtaatgga tgtgatgcag taagtaaaca
     1501 caaaagactt gtatcattag gagaaatatc agagaataag tcatcagatt acaaaattat
     1561 agtatctgta aataaaggag aaggtacgct taaatttatt gacaatggaa ttggtatgac
     1621 tgaagaagaa ataaaaaaat acataaatca agttgctttt tcaggtgctg aagatttctt
     1681 taataaatat aaggataaga tggaagagtc aaatgacata ataggtcatt ttggattagg
     1741 attttattct gcatttatgg tgtctaaaaa ggtacaaata gatacacttt catatacaga
     1801 aggagcaact cctgtaagat ggatatctga aggtggtaca gaatacgaaa tatctgaatc
     1861 agatactaga aatgatagag gaactactat aacactattt attgatgatg atagtaaaga
     1921 gtttttagat gaatttactg taagagggat aataaataaa tactgttctt tcttaccagt
     1981 agaaatatat ttagaagatg ttgaaagatt agaaagagaa gctaaggaag cagaagaaaa
     2041 agctaaaaaa caaaaggaag atggaaaaga agaggttgta gatgcaaaag taattgagcc
     2101 tttaaacgac actaatccgc tatggttaaa atctccaaaa gattgtactg atgaggaata
     2161 taaagagttt tatagaaagg tattccatgt atttgatgag cctttatttt ggatacattt
     2221 aaatgtagat tatcctttca acttaaaggg tatactttac ttccctaaat taaaaaatga
     2281 gtttgagttg actgaaggaa aagtaaaatt atacaacaat caagtgtttg tggctgacaa
     2341 cataaaagaa gttataccag aatttttact tttattaaaa ggtgttatag actgcccaga
     2401 cttaccatta aatgtatcaa gaagtttctt acaaaatgac agagacgtaa gtaaaatttc
     2461 taaacatata attaaaaaag tagctgataa gttaaaatct ctttgtaaaa atgaaagaga
     2521 agaatataat aaattttggg atgatataca aatatttatt aaatacggtt gcttaaaaga
     2581 tgaaagtttc tatgagaagg taaaagaatg tatattattt aaaactattg atgatgagta
     2641 tataacatta caagattatc tagaaaaatg taaggacaaa cacgaaaata aggtatttta
     2701 tgtaagtgat aaggagcaac aatctcaata cattaaatta tttaaagaat acgacttaag
     2761 tgctgttgtt ttaaactcat caatagatac tcattttatt tcatttatgg aatataaaga
     2821 aaatggagtt aagtttaata gaatagatgc agacctttct gatgtattaa aagataaaaa
     2881 tgaaaataaa gatagtgaag aaaacaaaga agagatagct aagatagaag gtctgtttaa
     2941 agaaactgtc ggagaaagag taaagaatta ttctgtagaa ggattgaaaa atgaagacac
     3001 tccagctatg gtgttagtat cagaacaatc tattagaatg gcagaaatgc aatcaagatt
     3061 tgctggaatg gatttaggaa tgaattttga ggaagaaaaa actcttgtta taaatgagaa
     3121 cagtccaata atcaaaaaat tggtttctct taaagatgat gaggaaaaga aagataaggt
     3181 tacgcttatt tgtaatcaga tagcagattt agctttactt tctaataaag aattaaagcc
     3241 agacgagtta gattcatttg tccaaagaag taataaactt atgagtttgt tgatagaatt
     3301 ataaggttga atttgaatac attgaagtaa atttataaaa agtattcaat atcaatgata
     3361 ataaagaggt gtctcaaagt gaacttttta gttcatgagg cacttttttt atgtgaaatt
     3421 aaatgtattt taatcgtttc aataatgaaa aagaaattta tctctatttt tagacagact
     3481 attttttata tttgattaat ttttaaatat ttaagtaggt tctaataaaa aactctattt
     3541 agttaaatat agttaacttg atatataata aaataataca tataattaag taaaagattg
     3601 ttttttagtt tatgtttaaa tacataatta aattttgtta aaaaataaaa ttattaataa
     3661 aatagaaata ggaggtatta gcatgaattt taattataat ttgccagtaa atatcttatt
     3721 tgggagaggc agaattaatg atgtgggaaa agaagtatcc aaatatggca aaaaagtatt
     3781 gatagtaaca ggtaaaaata gtacaaaaaa aactggatta cttgataaaa caatagattt
     3841 actaaaagac tctaaaatag aatatgaagt ttttgataga gtagagcaaa atcctcttat
     3901 aactactata tattcaggag tagaaattat aaaagcaact ggatgtgatt gtgttttagg
     3961 gcttggtgga ggaagtatta tggatgctgc taagagtata gctttttcct ataaaaaccc
     4021 aggagattta aatgattata tatttggaat tagacaaggt gaagaagctt tacctataat
     4081 cttagttcca actacatcag gcacaggtag tgaaggaaat tgttttgcag tacttacaaa
     4141 ttcagaaact aaagataaaa aatctataaa gaagaatagt atgtatgcaa aatcatcaat
     4201 aattgaccca gaacttatgg ttacaatgcc aaagcatata attgcatcag ttggatttga
     4261 tgcacttgct cataatatgg aagcatatct ttctaatgga agaaacccta ttgcagatgt
     4321 acaagctatt tatgggattg aacttatatc agaaaaccta gtaaaggtat ataataatgt
     4381 taatgattta gaagcatggg aaaaaataac acttgcaagt actattggag gaatgacgat
     4441 tggtacagcg ggaacagcct tgtcacatgg tatggagcat cctgtaagtg gtcttagaga
     4501 tgtaatgcat ggaaaaggac ttgcagcttt gactcctgtt attatagaaa gttcatggga
     4561 tagtgatact gaaagatttg ggaatatttc taaaatatta ggaggaactt gtgcagaaga
     4621 ttgttcagat gcaattagaa attttcttaa aaaaatagat ttggatataa aactaagtga
     4681 tttaggtatt gaaaaatcag atatagattg gttaactgat aattgtatga aaatatcagt
     4741 tgcaaatatt aagagacatc caaagtactt taataaggaa gaaataaaag aaatgtaccg
     4801 taaatctcta taatctgatt ttacatattt aactttaaat aaaaattata aattaataat
     4861 aaaaaattta taattaatca atagcaaaaa aattgtgtat aggaattaga tacacaataa
     4921 acttgtctta agcttttatt ttactttcta ataaataaag gagtttaaaa aagggaagat
     4981 taattaagta atattttata aaagtcttcg atttaaaaac tttaagtaga ggtgaaataa
     5041 atgtttaatc ccaaaagaat aatattcgaa aaaggaactt tagacactga tgtgggtaaa
     5101 aatatataca ataaatttaa agataataaa gatgtagaga taattaatat atcatcaaat
     5161 agaattaaag aacatattcc aggtgaaaat ctatatgaac aatatagaag tgggaagcag
     5221 actatggtgg ttgggattag aaaaagtctg aagtttcaaa catgtaaacc ttctgctaat
     5281 taccaactac cacttgtcag tggatgtatg ggaagatgtg aatattgcta tttaaataca
     5341 caattaggtg ataaaccttt tataagggta tttgtaaatg tagatgaaat actagaacag
     5401 acaaaaaaat atattgaaga tagaaagcca caggtaacca tatttgaagg agcggctaca
     5461 tcagacccag ttccagtgga gccatatact aattccttga agaaaacaat agagtttttt
     5521 ggaaaagaag aaaaaggaag atttagattt gttactaaat acagtgatgt agatagctta
     5581 ttagatgcca aacataataa tcatacagaa atacgattta gtatcaatac tcctagaatt
     5641 ataaacgaac atgagcatta tacagcatct gcaaataaaa gaatagagtc agctgtaaaa
     5701 cttgctaggg ctggctatca gattggtttt ataatagcac ctgtgttttt atatgaaggt
     5761 tggaaggaag aatataaaaa tcttattaga agtataaaag aagctttacc aaaggatttt
     5821 gataaaaaaa ttatatttga agttatatct catagatata caactaaagc aaaaaataga
     5881 atattagaga tttatccaga tacactatta ccaatggaaa atgaagatag aaagtttaaa
     5941 tacggtcaat ttggatatgg taagtacgtg tacgataaag aacaattaga tgatatgaaa
     6001 gagttctttc ataaggaaat aaatggagta tttaaaaata gtttaataaa atatataata
     6061 tagcaaatct tatctaaaga tttgctatat tttattttta taataaagtg atgagtttta
     6121 gtactataat atgagaaaat taaaaagggc actatctatc aagaacattt tttctagttt
     6181 tgatactata aaggataaga gtaacaaaaa tatcaggagg agttataata tgcatgcatg
     6241 ggagtctatt caaattacgt tggatttaat agaagcaaac ttatcagaag aaatatcaat
     6301 taatgaatta gcaaataaag caaacttatc accattttac tatcagagac tatttaaaag
     6361 attggtaaat aagactgtaa tggagtatat aaagcttcgt cgtcttgcaa aagcttctga
     6421 atacttagca gaacataaga atcgtatact tgatgtagct ttaaaatttg gatttgctag
     6481 ccatgagaca tttacacgtt cctttaaaaa agcatatgga ttaactccag aaaaatatag
     6541 agcaaatcca gtaaaactca atcactttat taagccagaa ttaattttaa attatgctat
     6601 ggttgatgaa ggtgttccac tgattgcaga tgatatagtg attgaagtca ctcgtaagac
     6661 attgagtaat ccaaggacgt ttgttggaat agaaatggaa gtacctattt gccagttggt
     6721 gggcggtgaa acaactggta ttgcaatagt agaggaatta tggatgaaac ttggaagtca
     6781 aagacataat ataccaaatc aaattccagg tggaaatgaa tttgctgctc tttatatggg
     6841 aaatgcaaaa gaaggaaatt gtatgtatat ggcaggtgta gaggtagaat tagggacatc
     6901 agtagaagga tatagtactt ttgagttacc agctaaagaa tatttagtat gtggatttga
     6961 ggcagaaaac tttaatgagt tagttaattc agctgttttt aaagcggata aatttatgga
     7021 aagatggatg aagaagcata atttaacaac tgctgatttt gcaattgaga tgtattaccc
     7081 cactacacct gaagtagcat atctggagca ttggattgtt ccaattccta ttgaacaata
     7141 gaaaggatga tgaatatgaa ttggcaagat acgttttttc aagatactaa acctacattt
     7201 gagcaaatta atgaatttat taataatcca ttatgggaca agttaaataa aactttggtt
     7261 tctacttata atgtagaacc taagcttgaa tacagtaaat gttcaatgca aagaggctgg
     7321 aatgtaaaat acaaaaaaag aggtaaatca ctatgtactt tatatccaca agaaggttat
     7381 tttaaagctt tggttattgt aaatgaaagc aatagagtag aggttgattt atttattaat
     7441 acttgttgtg attatatcaa acaaatatat aatgaagtaa aattttttaa tggtggtaag
     7501 tggttaatga ttcaagtaga tagtttgttg gtattaaacg atatgcttga actaattaaa
     7561 tttagaatat aaaatataaa acaggatgaa tatataagtc atcctgtttt tatattcaat
     7621 cagataaatt aatatataga ctatagtata aataatttag caaatttaaa tttactttgt
     7681 ttttctacat tccttgcaat taagtccaca tttatttttt tcttgcatat tggtagcacc
     7741 ccaattagca aattgttgta aaataggtac aatacttttg cctttacttg ttagtgtata
     7801 ttcaaccttt ggaggaattt cattatattg tttacgatga acaataccat cagactctaa
     7861 atcttttagt gattgagcta gcatagtgtt tgtaattcca tctaattttc tttttatgct
     7921 actatatcta atgggatttt caactgctaa catacatata ataggtaatt tccacttacc
     7981 accaacaata tctaatgcat aacgaattgg acaggttaat tcttcaacat ttttagttat
     8041 atttttttca ctatttatac ccattttaat tactccttag ttataatagt atctttttta
     8101 tactttataa gaaaaaactg catacttgtt ttttctgtat tttatattaa aattatatca
     8161 aaggataatc cttaatacaa gtatttaaaa ggggtgtaag aattatgaaa gcattagaat
     8221 ttttgaacaa ggcaggagtt tattatttag caacagctga tgagaacaat caagcacatg
     8281 tgcgtccttt aggatttgtt atggaataca atggtaaact tacattttgt acatctaatc
     8341 aaaaggatat gtttaagcaa cttattttta atccaaaggt tgagctttgt tgtatagacc
     8401 aaaaccttaa tactctacgt attttaggtg aagcagtatt tgttacatca aaagaaacac
     8461 aagctaaagc acttgaaata atgccaagtt taggtcagat gtactctgta ggagatggta
     8521 agtttgaaat attctctatt gataaggcaa aagttagttg ttgttcaatg agtggtaaaa
     8581 agattgatgt agaactttaa ttataattga atcgatacac atagtgaaaa ttgaaaaaac
     8641 gttatcactg aaatattgaa gatttaatta aaatgcaata aaatgttttt aaataatgaa
     8701 tggataaaaa tttttagtta ttagatttgt atttcaataa tgatatgtta ttttgaataa
     8761 ttacatacta gaaataaggg caatcaaaat ttattttata attggctaga cttcttttaa
     8821 aaaatatttt aactataatt gatttttatt taatcgaaaa aaatattatg actataaaaa
     8881 attgctactt attgtttgaa tatttttgta tataaataat aagtggcata aatttatata
     8941 ttaataatga tataatataa attaatagat aagatataat tttaaaagga gtgatataag
     9001 atgagcaaat atagttcttt atgggaatat gttaagaaaa acaatagtca atcgtttaaa
     9061 ttaacttttg aagaaataaa agatattgct ggagtagaga tagaccattc gtttttgaaa
     9121 tataaaaagg aattaaatga atatggatat caagtaggaa aaatctcttt aaaagagaag
     9181 actgttattt tcgataaaat tggttaagac ataaataatt acatataata aaataatttg
     9241 tattgacaac tattaaaaat aaagtaaatc ttatataaga tttactttat ttttagaggc
     9301 tgcttttttt aactttatac tataaggtat ataaaactaa gcctattaaa aatgttataa
     9361 tttctattgc tatcatagac catatcaatc catcaagttc ataaaagtaa ttaatcttta
     9421 ttaaatagct tctttattat aatttcttta tttcatataa tataaatagt attaaaattt
     9481 ttatgagagt tagagtaagc atgtattact acttaaatat ttctataatc aacatctagt
     9541 aatttacctt tttggtgaaa atgattaata agttttttgg gatgattcat tattaattca
     9601 tctggaaaat taatttcctt taagatattc tcagaatact catagttacc tatgtcaaaa
     9661 caaatgtgag catctgaagt tagtatgatt ttagcaccat attttttgca aagcgtagca
     9721 atatacttac agttatccga gctaccattt cttgacactc ctttaataga acaattattt
     9781 atttctatta agatattctt ttctatagct aatttaatga ttaattcata gtcgattggg
     9841 tagtttgggt ttccaaggtg tcctaatatt tcaatattat catgcttatt tattgcattt
     9901 attaaggctt tagtattatt ctctaatgta tttggtgaaa aaacggcttc gtgaaatgac
     9961 aagataagat aatctaatcg aggaattaca aaaggctcta ggtctatgtt tcctttagtg
    10021 tctagtatat ttgcttcaca tcctcttaaa attataatgt tatttatgat tcttgggata
    10081 tttttaatgt tgtgaaagta ccatctgtgg ggagaaccag gcatcttagg accatgttca
    10141 gatgtaccta gtatcttaat tccattttct ttacaataat taatattttc taacaaagtg
    10201 ctatagccat gaccactgac tatagagtgg gtgtgtaaat ctgataaaaa attcatataa
    10261 attcctcctc tataaaagat gagggtaacg taatttatat tataaaacga atagaaaaga
    10321 aaatcaatag ttacataatt agacaaaaat attaaaatat tgtagaatta agcttaataa
    10381 taaatgttta acagtattac tgcttatagg ggagggtaac tatgacacaa agaaagctat
    10441 caaggaaaga aaaagtttta atttgcttga aaaggttaac agaagaacac ttgaacttta
    10501 ataatggcat tagatttgga tttgatgcag aatatattgc tactgaagta ggtataaata
    10561 gaagtaatgc tagtaaggaa ttaaatcaat taataaaaga aagtattatt ataaagataa
    10621 agggaaagcc tgttcaatac ttatacaaag aaagtattga gagcatttta ggaaagaaac
    10681 taaataaagg gatatttagt aatattaact ctataaatga atatagtgaa gatgattcta
    10741 tcaaagatga gtatatctta gaaggtataa aaaaagagaa caatattttt aatttagtag
    10801 gatgtgatgg aagtttaaag tcccaaatag aacaggctaa agcagcagta atatatccac
    10861 ctaaggggtt gagtacatta ataataggac ctacaggagt tggtaaatct atatttgcag
    10921 aatatatgta taagtatagt ttgaatataa aagaagatag tgataatgca ccatttatca
    10981 tatttaactg tgctgattat tctgataatc agcaattatt gttagcacag ctttttggat
    11041 atgtaaaagg agcatttact ggagctgata aagacaagta tggtattgtg catgaagcta
    11101 ataatgggat attgttttta gatgaagttc ataggttatc tgcagaggga caagaaatgc
    11161 tatttctgct tatggataaa ggtatatata gaaggttagg agaggctaat aaggtacatg
    11221 agtgtagggt tatgataatt gcagctacta cagaagaccc agaaacagca atgctggaaa
    11281 cgtttttaag aagaatacct gtaactataa agattccgtc tttagaggaa agaggttttc
    11341 aagagagaat gaaacttata tgttattttt ttaaagaaga gagtattagg attggtgtta
    11401 agttaaaggt ttcaaaagaa gttattaagg catttatttt atataagtgt aagggaaaca
    11461 taggacaatt aaaaagcgat atacaattaa tttgtgcaag agctttttta gattatatga
    11521 cctataaaag ggaatgtgta tatgtaaggc tctctcttct accaaacaat attagagaat
    11581 cattatatgg aaataataga aaagaggagt ttgtacaaaa tttcagtttt attgggaaag
    11641 atgatattat ttttttacca gaagaaaaga gttttgaaag tgaaaattta ttaatagaaa
    11701 ataaaaaata tgatttagat ttctatggaa tgataaaaga aacttggaat aaacttcaac
    11761 aagaaggtat taaagagtca caaattagaa gtgttataga tgaagatata cagaagtatt
    11821 cctacaattt aatgaatact tttatctata atagtagtaa tcaaacagct tataacaaca
    11881 ttgttaatga ttcaatcgca actactgtga agtatgtttt acaaaagcat gataaatgga
    11941 gcaaaagaga aggattagat aaattaataa aagctatagc cttacatatt cagaatttaa
    12001 ttgaaagaat aaagattggg aacatagtaa agcatcctaa taaagatgaa attatgaagg
    12061 aaagagcata tgaatataac atagcaaaag aaatattatc taacatgtca ttaatgtatg
    12121 gagttgagtt tccagaagat gaagcaattt ttttagcaac gtttctttat ttatcttatg
    12181 tgggattaaa tgaggaaagt atagcaatat tagtcataat gcatggtgaa tctacagcct
    12241 ctagcatggt gaatgtagct aatactttac tggattgtaa ccatgcagtg gcaatcaata
    12301 tgggattaga agatagagtt caggatattt tagtacaagc tgtagaaata gccaatgaaa
    12361 ttgacaaagg aaagggaatt ttaatcttaa ccgatatggg gtctatttta acatttgcta
    12421 aagttattca tgatgctaca ggaaaagaag taaaagcaat agatatggta agtacaccta
    12481 ttgtaataga agcaactaga aaggctctaa ctccagaaat gacattagag aaattatatt
    12541 ttaatattat agaatctata aagaaacatt atggagatat ggaaattaca tatgctgaaa
    12601 aagataatgg tggtagatat tttgatagat tactaattga taatattagt aaaactttga
    12661 cttttttaaa cggggaaaag gcttatttta tattaaaaga agtaattaat agaattagtg
    12721 aacattattc tttagtaatt caagatgagc ttttagtaaa gtttatattt cattgttcct
    12781 gtatgattga aagagtaata ataaaagagt ctttagttta taaatcatat gaagaaagaa
    12841 ttagtagaag taaagaatta tatttagtta taaaagaaag ttttaagcta gtagaagaga
    12901 cattcgatat aattatttca gatatggaat atgcaaatat attagatata tttgaatcac
    12961 aatatgatac agataaattc aaaagttgat acaaaattta aaaatagcca aaaggctatc
    13021 ttttttttac tttttttgat acagatttga tacaagttgg aaaaatcttt tgatacatat
    13081 tgatatagta agttttttaa aaaatttaag caaaaaggtt tgattaaatc ctgtaaaata
    13141 gccatttgtt attgatacac ataagttggc acggcaattg cttgtataaa aaatgacaag
    13201 aaaagtgagg tgaaattggt gataggaata atagttatga ctcatgggag ttttagtgaa
    13261 gaaattataa aaagctgtga acttattgca ggaccagcag aaagaacagc agcaattaaa
    13321 ctaaatagga atgataatat tgaagactta aataaaaatt ttgtagaaaa gttaaatgaa
    13381 ctagatgaag gtgacggagt tttagtatta gctgacctat taggaggttc gccaagtaat
    13441 gttgcaagtt taaaccttaa aaaaggtgga aagtttcatg cacttactgg agtaaatctt
    13501 ccaatgctac ttgaagcttt aattaataga gagggaaaaa gtttagagga gttagcagaa
    13561 gcatgtattg aagcaggaca aactggtata aacaatatta ataaagttct tagctcaatg
    13621 taatagaaaa aggagtttga ttaaaaatgt ctttagtaaa atattgcaga tgtgatgata
    13681 gattaataca tggtcaagtg atatataaat gggttaagca tctaggtgta aaaaagatag
    13741 tagtagtaga tgatgaaacc accaatgatg ttattgctaa aggtttgata aaaatggcag
    13801 caccaaagaa tattgattta tcaatattaa ctgtaagtga atcaaggaga tatttttata
    13861 ataatcaagc tgatgataac gtttttgtat taattaaaaa tttagatact gcaaatagaa
    13921 tgatagaaga agggattaat atcaaaaaac ttattgttgg aagaatacct acaggaatag
    13981 gaaaaaagaa aatatctcaa aatgtataca ttaataaaaa agagtttctt ttaatagatg
    14041 aatttattaa aaaaaatatt aatgtatcaa ttcaaatggt acctgacgaa gaagaaatag
    14101 aattaaacca caatattaat cgctataaag gagagtttat ttagcaatga ataaatataa
    14161 ggtaatcata ctctgtcatg gaacttgggg aaagttatta gtagaagaaa caaataaaaa
    14221 ctttggattg tcaaatcaat atgaagtttt atcattaagt tctgaaaaag atgtttctgt
    14281 attcatgaaa ggggttgaag aagcagtaga taaagaagat gtgaaaatta taatatcaga
    14341 tttatatgga gggtctacat caagtgttgc aatatctgtt gctataagaa gaggaataga
    14401 tgcaataaat ggacttagct tacagacaat tttaatcgta gatgaggaat taagtaaaga
    14461 agataaatta catacattac cagaaagaat agtcaaaaaa aataataatc tttgtgtgga
    14521 tttaattaaa gagtttaaaa aaatataaat tatgaggtga atataatggc aaaaataaca
    14581 ttagtaagag tagaccacag attaattcat ggacaagtta taacaaaatg ggtaaaaatc
    14641 gctcaagctc aaaaaataat aatagttgat gacttcttag gtcaagatga atttatggct
    14701 gacatatata aaatggcggc accatcagga gtggaagtag ttattttaac agcagaagat
    14761 gcaggacaag cattccaaaa caacacttta ggagataaaa atatatttat tctatttaaa
    14821 aatgttgata tggcaaataa ggcttataaa gctggactta aatatgaaaa gattcaatta
    14881 ggaggtattc caaatgaagc aggaaagaag atggtattta cagctgtttc attaggaagt
    14941 gaagatataa aacaacttaa tgaacttaat gaaaatggag ttgagatagt attacaagtt
    15001 ataccagagg aatcatctat gacatatgaa aatgcattaa agaaatttaa ataaaaaata
    15061 taaattagga ggaattataa atggaatctg ttttgatgtt ggcaattgta acaggattat
    15121 ggtattggtt tgcagcaggt cttgcagggt ataccctttt ctcaacacta aagtctcctt
    15181 tatttatagg ttttagcctt ggattacttt ggggtgatgt aacaactggt atgattgttg
    15241 gtgcaagtat agaaatggtt tatctaggaa tggtcgcagc aggaggaaat attccatcag
    15301 ataaatgtct agctgctctt attgcaatac cagtagcact tcaaactggt gttaatgcag
    15361 aagttgcagt atctattgcg gtacctttag gcgttatagg agtattagtt aataacttaa
    15421 gaagaactgg taatgctgta ttagttcata aggcagataa gtatgctgaa gaaggtaata
    15481 caaaaggtat ctggagatgt gcaacacttt attctttgat atttgggttt gttttaagat
    15541 ttccaatagt ttttgtatgt aacttttttg gagcagattt agttcaatca ttattaaatg
    15601 ttataccaca atggttaatg aatggattaa cagtaatggg aggtatatta cctgcattag
    15661 gttttgcaac aacaatattt actataggta aaaataaatt cttaccaatg tttattatag
    15721 gattttttat ggttcaatat tttgaaatat caattactgc agctgcaata tttggtgttt
    15781 gtatagctct tttaattacc tttatgaaag aagacaaaag ggtaggtgaa gtataatgca
    15841 agaaataaaa aataaagacg ttaaaaatgg acaaggtaca actgaagtta ataagacaag
    15901 agtacttaca aagaaagatg taacaaaaac atacctaaga tggtggtgga cagcagaatt
    15961 atctaactct tttgaaagaa tgcaagcatt agcagtttgt gcaagtttca ctccagcttt
    16021 agaaaagtta tataagaaaa aagaagattt agtagatgcg ttaaagagac acttacaatt
    16081 ttttaatact caagctatat ggggagggtt aattcacggt acagttttag ctatggaaga
    16141 agagaaagct actgaaggaa aaatcccagg agaggtcatt tcaggtgtta agaatggtct
    16201 tatgggacct ttggctggaa ttggagatac tttagatttt ggtacatttc aaacaatatt
    16261 cttagcgtta ggtgcttcat ttggagcaga aggaagtgtt ataggtgcat ttttcccaat
    16321 aatctttagt atattactat tttgcgaagg ttattactta ttccatttag gatattcttt
    16381 aggtagagat tcaattaaga aaattctatc aggtggtatt gtcaataaga taattgatgg
    16441 cgcttcaatt ttaggaatgt ttatgatggg agcattatct gcaactacag ttaagctttc
    16501 aactccgcta tcatttgata ttggtggtaa agcaattgtt gttcaagata cattaaatat
    16561 gattgctcca ggtttattac cacttagtgt tgtattcttc gtatattggg gaatgaaata
    16621 caagaagtgg acaataacta agttattagt aattttagtt gtattggcat tagtaggatc
    16681 attcattgga atattctaat ttacaatgat taaaatatag aatttaattt taggaggaga
    16741 aagaaatgtc agaaaaaatg tatacagaaa aggaatattt agaaggaatt agaggagcaa
    16801 caggagaaag agcagtatgg ttctatcttc ttatgaaaga agctgaaaaa ttaggagtta
    16861 atccagatga tatatgtaaa gaagcaatat atggatttgg taaaatgaga ggtcaaaaat
    16921 atagcgtagc agacactcca ggaaaaatgg cagaaatgtt atataacagt aaaggacaaa
    16981 aagtatttga aatggaatta gtagaaaata cagatgagaa tggagtttta aaattccatc
    17041 actgtccatt agatgcagcc tggaaagaat atggattaac taaagaagaa agaaaagaaa
    17101 tttgtagatt agcttgttac ggtgattatg gaagagttga ctgtgcacaa agtgttagat
    17161 tagagtttgc tcaaaaacgt gctcatgatg atgaagtttg tgaattagta ttcacaagaa
    17221 aataattttt aatgctaaaa gcctgttctt taaggacagg ctttaaaaag atagaaagta
    17281 ggttacttta tggatataag cagaagagta gataatagtt taaatagaac catagaattt
    17341 ctaaaagaat taataaaaat tgactcacaa caaggtgaac ctattttaca atgtcctttt
    17401 ggtataggac ctaaaaaatc tttagagaaa actttagatt actgtgctag tttaggtttt
    17461 agtgttaaga atattgataa ttatattgga tatgcagaaa ttggagaagg aaaggagtta
    17521 atagggattc caatgcattt agatgtagtt ccaccaggag acgggtggag cgtagaccca
    17581 ttttcgggag tagtaattga caatattatt tatggaagag gtgccgtaga taataagggg
    17641 gctgtatcta tgcttataca tgtattgaaa aacatagagg atatgtaccc tacaattaat
    17701 aaaagaataa gattaatttt tggtacaaat gaggaaacag gcatggaatg tatcaaatat
    17761 tacttagata aaggtgaaga aatcccaagt atgggattta ctccagatgc tatgtatcca
    17821 gtagttaatg gagaaaaagg aagagttcat ataaggatag aaaaagagat taaaattgat
    17881 aaaagtaaac cttacataat attaagggga ggaactaaag aaaatgttgt tccatctcat
    17941 tgtactgcaa aaattgttaa tggaattatt ttagagctaa ccactaaagg agtagctgta
    18001 catgcaagta atcctgaaaa gggagaaaat gctatatcta aaatggtaat aaagatattg
    18061 aaagataata tgaattttca gcatagagaa gatatagaat tagtttcaaa atacctttgt
    18121 agtgactatt atggaaatgc attagggatt aatcgatatg atggggtatt caaaaacact
    18181 accttaaatt taggaatatt aaaagtaaat gaggaaaaaa ttgtatgtga gttggatata
    18241 aggtatggta aaaatataat tttaaacaat ataatagata gattcaaaaa gtttttttgt
    18301 aatggttgga aaatagaggt tattgcccat aaagacttac attatgtgga tgagagtaat
    18361 ttagtactta aaaaattatt agaagcatat gaagaagtaa cagatgaaaa tggatataca
    18421 attgctatgg gtggtggcac atatgcaagt tggtttaaag atatggtggc gtttggacct
    18481 aagtttttag cgtataaaac tggtggtcat ggagtagacg aaagagtacc aataaatcat
    18541 atacgaaaga atatggagat atataccttg gctttaataa aactattaga attgtaaaaa
    18601 gagaagagat tttatgtgaa aatgtacgga tatcttaaaa agagagataa aacgagttta
    18661 actttattta aatgttcaac accatcaaat ggggctgtct caaaatatag ataagtctct
    18721 tccttattgt tgaaatgatg aattttactt catacaaaaa agagtgtctt atgaagtaaa
    18781 atgttcattt tgaaacacac tcatttgatg atgttgaaga gaaaataagt atgaaattat
    18841 taaaaaagat gattttataa aagttaaggt tgaaaagtta attgaatgga attagctaaa
    18901 tggagaaggc tatatactat ctaataaaat aataaaaaag tgttgtaaaa ttggttatat
    18961 gattagagaa aagtctattg atatttattc agacagtgga tgggaattta tggtttggga
    19021 tgaaaatgga gaataataaa aagtatagtt tatacaattt aataagatat atgtacaagc
    19081 agttaatata aatgattgac tgcttttctt ttatagattt ttatataata gacaattact
    19141 aatacatgat agttgcctat tataattata agaataaata cgaataataa ttttataaaa
    19201 gaattaaatg gaaaattgaa gagttatatt caggatttta aaaagaatat gaaatgagaa
    19261 atgaaaataa ggtgctttat attaaaagat attttataca aaatatttaa aaacgtgata
    19321 aataaaactg tatttagata gattaattac tagttttata tagaatatgg attttgttta
    19381 caaaattaga atattttaca agttattttt aaaaaatatt taaatatata ttttagatat
    19441 ttgtttctct gtggttgatt ttttcgtcaa aagtcaactt caaatcgctt gttagcttta
    19501 tgtgtttaag gatataatta tacataagga gggattgaca tgaatgaatt aaacatcgca
    19561 aaaacgctga ttttaaagcg taaagaaaaa ggaattacac aagatgaact tgcaaattat
    19621 ataggcgttt ctaaagcttc tgtatctaaa tgggaaactg gacaaagtta tcttgacata
    19681 acctttttac cgcaactcgc gacatatttc aacattacag ttgatgagtt aatatgctat
    19741 gaacctcaaa tgatgaagga agatattaat aaactataca ataaactgtg caaagacttt
    19801 acggctaaac catttgatga agtcatgatt gaaataagag aaatcataaa aagatattat
    19861 tcatgttttc ctttgatttt tcggatgggt cttttaatag tcaatcacta tgacattgta
    19921 gatgaaaaaa agcgtgaact attaattgat gaagcattgg aaatatttat tcgtatacag
    19981 gaaacttgta atgatataga tatttgccgt caagcaaaaa atatggaggc aacttgttat
    20041 atattgttaa atcaacccat acaagtgatt gatttattgc aaaatagcaa ttttcccatg
    20101 ataaatgaat ctatacttct tgcacagggg caaatgatga atggtcaaat tgatgaagca
    20161 agagaaacat ttcaacttgg ggcatatcag aatcttatat cactagttca aaatctagtg
    20221 ggtattttgc aaaatgcaga taaattacaa atgaaagaaa tagaaagacg tattctagca
    20281 atttcaaata tatttgaact tgatacatta tctcctgcta ctatgctttc tgcatattta
    20341 acacagacac aaattaattt aatacatgag gataatgaag gagctattaa atcattacga
    20401 aaatatgtag atttagcaac aagtgatata tacccaatta caatacatgg tgatgacttt
    20461 tttgataaac ttgacagatg gatttctgaa attggaatag gtattacacg tgatgataca
    20521 atcgtaaaag cgggtattgt tgctgctatt aaaaataatc caatgttttc tgtattaagt
    20581 gaaaacaaag aatacaaatt tcttatagaa aaattatctt tattggagga ataagattat
    20641 ggaaaatttg atggaatact taccactttt gattcctgtt attattttag atttaattct
    20701 tataattact gcacttgttc atgtattgag acatccaaat tataaaattg gaaataaagc
    20761 aatatggatt attgtagtgc tgtttattag cataatagga ccaattttgt attttacaat
    20821 aggaagaggt gaagaatgat gaatattgtc tcaataaatg ggttatcaaa aggttttggt
    20881 aatcgaaaga taatagataa tctaaatttt actgtaccag aagggtcagt ctttgggttt
    20941 gtaggtaaga atggtgcagg taagactaca acaatgaaaa tggtacttgg gttacttgaa
    21001 ccagatagtg gaactataga tgtttgtggt gagaaagtga cctatggagg aacaagttct
    21061 aatcgtcatg taggttatct gcctgatgtt ccagagtttt acaactatat gagaccactt
    21121 gaatatcttt ctctttgtgg cgaaattacg ggactttcaa aacaagaaat aaagataagg
    21181 agtgaagagt tgttatcact tgtgggactt ggcaatgaaa agagacgaat aggtggattt
    21241 tctcgtggaa tgaagcaacg acttggtata gctcaagctt tgctttcacg tcccaaatta
    21301 cttatttgtg atgaaccaac aagtgcactt gaccctgttg gtcgaaaaga aatactagat
    21361 attatgctta aaatcaaaga ctcaacaact gtaatatttt caacacatat actatcagat
    21421 gttgaaagaa tctgtgacca tgttgctata ttaaacaaag gaagcattgc tcttagtggt
    21481 acactttcag aaataaaaag tatgcacgga aaggatagac ttttactgga gtttgcaagt
    21541 aatgatgaaa ttcaaaagtt taagtctagt ggtgacatta aatccttact aaaatattca
    21601 gaagaaacta atatggaaat tgttttgcat ggtaaagata taaaagcaat acaaaaaaca
    21661 gttatatcta cactcgcaga aatgaatctt tgtccagtaa agatggaact aatagaattg
    21721 tcacttgaaa atttattttt ggaggtagta aagtgagaga atacatagca tttacaaaaa
    21781 aagaatttaa ggagaactta agaaattata aattatttag tttgattatt ttatttttag
    21841 tatttggaat aatgagtcct ctttctgcaa aatttatgcc agacttgatt gctcattttg
    21901 ctccaacttt aaaagttact actgcaccaa ctgctttaga ttcttggaca cagttttctg
    21961 gcaatatttc tggacttgga gcgagtttaa ctctaattgt tttttgtaat attttatcaa
    22021 acgaatactc taaaggaaca ctcgttatta tgttgactaa agggttatca cgttcatctg
    22081 tgatattgtc taaattttct attgcagtaa tcattatgac aattggtttt tggttgagtt
    22141 ttttatgtac ttatggatat acaatgtatt tttggccaac tgaaaatctt aatcatatta
    22201 tattttcagc atttaactta tggttaatta atattatgta tattaccata ttaattcttg
    22261 ggtgtgtatt atttagacca gcatttgcaa gtgtgttact tgtattagta gcaactctta
    22321 ttttgagttt gatttctatt cctaaacaga ttgcacctta tacgcctaat ttcatattat
    22381 caaaaaatat agatttggtt tcaggtaaag tggcagctcc agaatttatt attccaatga
    22441 ttgtaacaat tgttatttcg ataatatgtt tgttattagc tgtcatttta tttaagaaaa
    22501 aagcagttta aggatatttt tattattaaa tataaatagt actgtattat ggaaaaatgt
    22561 gtttagtgtt gtagaagaat atatttaagt aaaaagaggt tgtctcaaaa tagagatata
    22621 agcctctttt ttattgttaa aattataaaa acatatttga tttcatacaa aacaagtatc
    22681 tcataagcta aaaaattcat tttgagacat ttcttttttt attttagtca ttctcattgt
    22741 actttataat aattaagtta ttaccttagg gaattgttta aaaataaaaa agagaaggat
    22801 tataatctaa tgattataag ttatttaagc tatgtttagt atatagagtc taaagtagta
    22861 aaggattaaa ataatattgt ttatatcaaa taaagataag aatctttaat tgtggtaaaa
    22921 tatactaaaa aatcacttta aaagtgataa aattatttta agtaaatatt ttaaaggggt
    22981 gtagatatgg aaaagtcaaa agtatatttt tgtgatttat attcaaattc gcaaaataaa
    23041 aatgtaccaa ataatgtaag aagattattt gataaagcag gattcaagga tttaatagaa
    23101 aagaatgacc aagttgcaat taaacttcac tttggagaaa aaggaaatac tacatatatg
    23161 agtcctgtgg cagttagaca agtggttgat aaggttaaag agtgtgaagg aaagccattt
    23221 ttaacagata caaatacact atatacaggg agtagaacaa attctgtaga ccatcttaca
    23281 actgctatag aaaatggatt tgcttatgca gttgtaaatg caccagtaat aattgcagat
    23341 ggattatata gtagaaatta tgagaatgta aagatagata aaaaacattt tgaaagtgta
    23401 aaaataggtg gagaaattta taattcctct gcaatgatag taatgagtca ttttaaaggt
    23461 catgaagcag caggatttgg aggagcatta aaaaacctag ctatgggatg tgcaagtgca
    23521 gcaggaaaac aaatgcaaca ttctgatgtt accccagtag taaaggaaaa gaagtgtata
    23581 ggttgtggaa agtgtgttaa ttcttgtcct acaaaagcta taagtatagt ggataaaaaa
    23641 gcagttatag attctgatgt atgttatgga tgtggagaat gtccaactgt ctgtccaact
    23701 agagcagtaa ctatacaatg ggaaagtgac tcagatgtgt ttgtcgaaaa gatggcagaa
    23761 tatgcttatg gagcagtatc aaataaaaaa gacaagatag gatatataac ttttgttatg
    23821 aatgtaactc cactttgtga ttgtgtacct tggtcaggta gaccaattgc ccatgatata
    23881 ggaatattag catcaacaga tcctgtagct attgaacaag catgttacga tttgatttgc
    23941 aaggaaatgg gtcatgatgt tttcaaacat gaacatcctc atgtaaatgg aactagaata
    24001 atagattatg catgtgaaat gggtatggga agtaaagaat atgaattaat aaaattataa
    24061 gaatagtagt acttaaatgc atataattat tgtaagagaa atctaaaagt atcaaaggag
    24121 tatgcattta tgaaaattta ctatgagaaa gaaaacagca agaaggccaa tgtaaacaat
    24181 attccaagtg tatttgcgtt taattttcta agaaactttt tgactagcaa agagtatgag
    24241 gaaactagaa aagaatattt tataaataat ctaacaaaaa gtcaggttaa tcaagctatg
    24301 aaggatttaa aatggctatt tagagaatat gaaggcttag aggtcattac aatggaaagc
    24361 ttggatggag ttaagacaag gatagtttta taaactagca agaattatat tttaataaaa
    24421 aatactgttt caataataat atgtataatc catgtactaa tattattttg aagcagtatt
    24481 tttttgcaaa tagtaaactt atacataaaa taaagttgac caataaatat taagtatgta
    24541 atactatata taataggtaa actaaatata ggtattaagt tgacaaatag gagggtatat
    24601 ggaaaaatac ataataaaat taaaaaataa agtattggga ggaaaagaaa taagttatga
    24661 agaagctctt aatttaataa gtcttgatac aaataataag aacgattttg atattctatt
    24721 aaaatctgca aatgaaataa gagaattttt tatgggaaga aaagcagatt tatgtacgat
    24781 aattaatgct aaatctggca aatgtagtga agactgtaaa ttttgtgccc agtcatctca
    24841 ttataaaact ggtgtagaag aatattcatt gttagattac aatgaaatct taaatagagc
    24901 taaagagatg gagagtaaag gagtacatag attttcattg gttacaagtg gcaaaggtat
    24961 gtcaggaaag gagtttaatg atattttaaa aatctatgaa agattgaggg aaaatactaa
    25021 tttaaaatta tgtgcatctt taggaattat agattatgag caagctaaaa tgttgaaatc
    25081 agtaggtgta actacgtatc atcacaatgt tgaaacggct agagataatt ttcataatat
    25141 atgtacaaca cacacttata aagataggat aaaaacgata aaagaggcta aagaagctgg
    25201 gctagatgta tgtgtaggtg gtataattgg aatgaatgag agtgaagaac agagactaaa
    25261 aatggctttt gaaatacgag aattaaatgt taaatcattt ccaataaata ttttaaatcc
    25321 tattaagaat acaccaatgg aagattatga tgtattagaa ccaatggaaa tattaaaaac
    25381 aacagcagta tttaggttta taattcctaa tgtatatata agatatgctg gtggaaggct
    25441 atcattaaag ggatatgatg aagttggatt taatggagga gtaaattctg ccattgttgg
    25501 tgattatttg acaactgttg gaaatggcat tgaaaatgat aaaaaaatga ttatagagca
    25561 aggatttgaa atttaataaa tattacttaa attaattaat atacttttag ataattataa
    25621 aagtatatta attttttttg tggattaatt taaatattaa atttgaatta taaattgcaa
    25681 tattttgaaa aaagggaaaa atatcaaaat ttatataaaa tagttgcaaa ggaaaaaaag
    25741 atgtgatagt atctaggtaa ccggttaagt aaaacggttt cctaaattgt gggggtgaac
    25801 ttcttgagta taacaataaa agaaattggc gaattggctg gtgtttctaa aacaactgtg
    25861 tcaaaagtca ttaataataa agatgaaaac ataagccagg caacaagaga aaaaattttg
    25921 aaaataatga aagaaaaaaa ttatgtgcca aataaattag cacaaagttt agtaactaaa
    25981 aaaactaata ctatagggct tcttatacca gatatacgaa atccattctt tacagatgta
    26041 tcaagaggtg tagaagataa agccaataaa gaaggctaca atataatttt atgcaataca
    26101 gatgaagatg ctaaaaaaga atatgaagga ataagaacgc tatcagagag gatgatagat
    26161 ggaattatat ttgcagcctc ttcaaataca aactggaaag aagcaaatta taaagatata
    26221 aaaataccaa ctgtgttgat tgataagaag ataagcatga acaaagaaac cttaaaagga
    26281 attgttaaaa taaataactt tgaaggggcc tatatagcta caaaatattt attagatact
    26341 ggaaataaga aaataatata cctaagtggt ccacttcaaa atgaaatagc tatagataga
    26401 ttagaaggat ataaaaaggc tctaatcgag tgtaatttaa gctacaatac tgattatgta
    26461 tttgaaggaa aatataagat tgaatgggga caagaattta taaaaaattt agaaaagata
    26521 gattttgatg caatattttg tgcaaatgat ttgattgcaa taggtgtcat aagaggtctt
    26581 aaagaaagag gattaagtat accaaatgat gtgagtgttg tgggatttga tgatattcaa
    26641 acatcaagtc taataagtcc ttctcttacc acagtaagac aaccatctta tgatatagga
    26701 tataaagcca gtgaaatatt gataaattgt ttaaagggag ataaaaaaga atcgtttgat
    26761 gaattgattt tcaaaccaga actagtcata agagattcga caaaagaaag tgaataacag
    26821 catgaaagac ataaatagat atgtaaagga gtaatgtaat gggaaacata gttgtaatag
    26881 gaagtgtaaa catggacatg gtatgttctg tagataaaag accagaaaaa ggagaaacag
    26941 tattaggtaa tagttttttt acatcacctg ggggaaaagg tgctaatcaa gctatctcag
    27001 cttctaaact aggagcaaat gtaaaaatga tatcatgtgt aggggaagat agcctaggag
    27061 aggagttaat aagaaatttt agaaccaata aagttgatta tagcttagta tccagaaata
    27121 agcataaaag ctctggtgtt gctgttataa cattatgtga aaatgataat agtattgttg
    27181 ttgtaccagg gacgaatgag ttagtagata tagaattaat taagaagaat gaagaagaga
    27241 taaagaatgc agatatagta ttgttacaat tagagattcc attaaaaaca ataaattatg
    27301 tagtaaattt ctgttttgaa aatagaatta atgttttatt aaatccagca ccagcaataa
    27361 aattagatga agatataata gagaaagtaa cttatttaac accgaatgaa catgaatata
    27421 agatagtttt tgacacaaat gaagagatag aagaagtatt aaaaaaatat ccaaataaac
    27481 ttgtaataac agaaggaaaa aatggagcta gattttatga tggaaatgaa gttaaacatg
    27541 tatcttgtat aagtgttgat gtccaagata ccacaggagc aggagataca tttaatggag
    27601 ctttggcagt agccataaca gaaggtaaaa atctatatac agcagtagaa tatgcagtag
    27661 tagtatcagg tctttctgta acgaaattag gtgcacaatc tggtatgcct tataaagaag
    27721 aggttgaaaa atatttaaat aacaataaat aaaaatctaa tttgttcaaa tttaaaacta
    27781 ttaaaagtgg aggttaatta tgaaagtgag aaaaatttta tctttaggaa ttgctggaat
    27841 actagcaata ggaatgttaa cagggtgttc aatggaagga ccaagtaaat cagacaataa
    27901 aggtgcttca gacaagaaag atttaactat tggagtatct acaataactc ttcaacatca
    27961 attttttata gatattgatg agggaataaa agaaaaagct aaagaattag gtgtaaaagt
    28021 tatagtaaat gacccagacc aagatgtagc taagcaaaca tcagcaatag aagattttat
    28081 acaacaaaat gtagatggaa tgatagtact tggaacagat aactcggcta tagtaccggc
    28141 agtagaagga gcatttgaaa aaatgcctgt agttacagtt gatgctgtat taaatactga
    28201 aaatattaca agttatgtag gaacagttag ttatgatgct ggtaaaaaat taggggaata
    28261 cactaaaaaa tacatagatg aaaaattagg tggaaagtca gaaatagcaa tagtaacaga
    28321 tttaaaatct caaatacaaa tgcagagaat agatggattt aaagatgcat taaaaggttc
    28381 agcaaatgtt aagatattaa attcacaacc aggatatgac agagaagaat ctcttaatac
    28441 agtagaaaac ttaattcaat ctaatccaga tgtagatata atatatgcaa cagctgaaaa
    28501 tagtgtacta ggagctaaag ctgcacttga atctgcaaaa aataaggatg ttaagattgt
    28561 aggatttgac ttaactgaag aagcatcaag tggaataaca gatggaacta ttcttgctat
    28621 gattcagcaa cagcctaaag agatgggacg tttagctgta gaagcagtag taaaagctat
    28681 aaaaggggaa aaggtagaaa aaaatatacc agtcccagct ttactttatg ataaggaaaa
    28741 tataaaagac tttaagaatt agcaaaaaaa atgagattat ttgaaagaaa tcctatttaa
    28801 aaaggagtgt cattatgagt aatataattc tgaagttaag caatattgct aaagaatttc
    28861 ctggtgtcaa agcattagat aatgttaatt ttgagttatt tcatggcgaa gttcatgcac
    28921 tactgggtga aaatggtgct ggtaaatcaa caatgataaa gattttaact ggtgcacatt
    28981 cgaaaacttc tggaaaattc atttttcaag ataaagaaat agagaatata tccccagatg
    29041 tctctaaaaa gataggtata aacgccatat atcaagaatt aacagtcttt gatgaattaa
    29101 cagtagcaca aaatattttt atgggaaagg aaataaatgg caaagtatta accaatgaca
    29161 aaaaaatgaa tgaagaggct aaaaagattt tcgataatat gggaatagat gtaaatccaa
    29221 atagtctagt caaagagctt agtatagctc aaaaacaaat ggtagaaata gcaagagttt
    29281 tatcatcaga aactaaagta ttaataatgg atgaacctac ttcttcaata agcaaaaaag
    29341 agactgaaat actttttaga cttataaagg acctaaaaga tagtggagtt agtataatat
    29401 atatctctca taggatggaa gaactttttg agatttgtga cagaataact ataatgcgag
    29461 atgggaaaac ggtttcgact ttgaatacca aagatgtatc atctgaggaa gaattagtta
    29521 atttgatgat agatagaaag ttagaccaat tttttccgaa gagaaaagta gaaataaaag
    29581 aagaaattat gagagtggaa aacttaacta agaacaatgt ttttaatgat gttagtttta
    29641 atataagaaa aggcgagata ttgggaatag gtggactagt tggaagtaag agaagtgaga
    29701 tagtagaagc tatatttgga cttagaacat atgattctgg gaaaatatat ttaaacaatg
    29761 aagaagtaaa gtttaaaact ccatcagatg caatagaaaa tggactagga ttgataacag
    29821 aagatagaaa aggtactgga ttgtttttac aaatgagtgt caaagaaaac acaactatgg
    29881 ctgggttaaa aaagatatct aagtttaaat caattataga tagaaaaaag gaaaaagaga
    29941 ttttagaaaa atacatagaa gctttaagga taaaaacacc acatatgaat caggttatac
    30001 aaagtttaag tggtggaaac caacaaaaag ctataatagc aaggtggctt ttattacaac
    30061 cagatatatt aattatggat gaaccaacca gaggtataga tgtaaatgct aaggcagaga
    30121 tatacaatct aatgggaaat ttagtagaga gtggagtaag tataataatg atatcttctg
    30181 aaataccaga attaatatca atgagtgaca gaataatggt tatgagagaa ggtcatataa
    30241 gtggattttt agaaggtgaa gaaatggttg aaaataatgt attaaaattg gctttcggag
    30301 gtaaaattaa tgagttcaat aactaagcat aaagaagaaa aatcttctcc attattgaca
    30361 aaattaacaa gtgcatttag agaccaagga agtgcagctg taggacttgt aataattttt
    30421 ataataatga gtatagcttc atctaacttt ttaactttag ataatttaat taatgttgga
    30481 agacaaatat caataaatgc aatacttgca gttggtatga catttgtaat aatcacagga
    30541 ggaatagatt taagtgtagg agcagtaatt gctttagtag gtacattttg ggctacaaca
    30601 gtagtgaatt ataatgcacc tatatggtta ggtatgatac ttgcattagc aatagggaca
    30661 atactaggag tcataaaggg tgcaattatt tcaacacaaa aactaccacc attcattgta
    30721 actttagcca tgttaacgat aataagtggg gcaagttttg tatttacagg aggaagacct
    30781 atatcagtaa atacagatgc atttaagatg ctaggtagag gatatgttgg accaatacca
    30841 ataccagtta taattatgat tatagttgta atagcaggac attttctgtt aaaaagaact
    30901 aattttggac gtcatgtaca tgcagtaggt ggaaatgaag aggctgctag attatgtgga
    30961 gtgaaagtaa ataaagttat agtaaaagta tacgcactag ctggactact aacagcatta
    31021 gctgggataa tactatcatc acgtttggca tcaggttcac caactgttgg agatggagca
    31081 gaattggatg ctatagcagc cgttgtgctt ggtggtacta atatgatggg tggttctggt
    31141 tctatagtag gtacatgtat aggtgttggg ataataggaa tattaggaaa tggattaaac
    31201 ttattaagtg tatcttcata taatcaaatg attataaaag gtttggtaat gttatttgct
    31261 atatggataa ataatataaa attaaaaaaa ttagctaaat ctaaatagca attaaatttt
    31321 agtttataaa ctataaaaat tattaagttt ttgtatatat ttttatactt taatttgcat
    31381 atatctttat acattaaatg ggaatatgct aagtgaaaaa gtttactatg gcttaaatag
    31441 atgagattag acttgaaatt ttaaatgtaa aaataaatct taggaggaaa tatgaaaaaa
    31501 attctcttag atacattaaa tagtaaaaaa caagaatata tagattattt aaaagaactt
    31561 gtaagtataa agactgaaga tgttggtcat ggaatactag gtggatttga aaaagaagga
    31621 caggaatata ttgaaaaact tgcaaattac ataggtttta gtgtagatag acaagaaatg
    31681 agcgaagaat taattaaaaa agcaaaaaat atatataaag aaggaaatct aggtcacaat
    31741 tatcaagata gatataattt gatttgtaag tatagtgatg atttacctgg aaagacaatt
    31801 gtatttaatg gtcatgtaga cactatgcca ccaggtgata taagtaagtg gaaatataat
    31861 ccatatcgtg ctacagaaga taatggaaaa ctttatggtc ttggaattgc tgatatgaaa
    31921 tctggactta tagcatcaat attagctgta aagcttataa aagatagtgg tctaaatgtg
    31981 cctggtaatg taaaaataat gtcagtagta gatgaagaag gtggaggaaa tggtaccata
    32041 aatgcagtta tgaatggtat tgatggagat tgttgcataa tttgtgaacc atcagaacat
    32101 aacttaatag ttgcacatat gggatttgta ttctttgaag ttgaagttaa aggtgtgtct
    32161 cttcactgtg gcagtaagtg ggagggtata aatgcaattg agaaggctat gttgttgctt
    32221 caagatataa aagagttgga gcataactgg cttatgatat ataagcatcc actattacct
    32281 tcacctacaa taaatttagg tgtaataaat ggtggaacag caggctctac tgtgccagat
    32341 aagtgtgtat ttaacttatg tgtacatttc ttgccaaata taatgagtta tgaacaagtt
    32401 gtaaatgatg taacaaatgt cataatgaca agagctaatg gagatttgtg gcttaaagat
    32461 aataaaccaa atataaacat atatcaatca gggttaggct ttgagatgga caaggattct
    32521 gattttgtag tgaatgtaca taaaatacta gaagaaactc tagggagaag cttagaaata
    32581 aaaggttcga cagctggcaa tgatgcgaga ctaatgaaaa atttagctga aataccaaca
    32641 cttatattag gaccaggttc aatagaacaa tgtcactcaa ttgatgaata tgttgaaatt
    32701 aaggagtatt tagatagtat acttatgtat gcaagtctaa tattaaactt ataaaagtag
    32761 gaggaatata taaatgaaaa aagtattaat agtaggggaa tcttgggtaa aaaatataac
    32821 tcatataaaa ggatttgata catttgttac aactcattat gaagaagctg taaagtggtt
    32881 gaaagaagca atagaatctg gtggatatga gacagttcat atgcctgcac atgtagcagc
    32941 agatagtttt ccttataaat tagaagagtt aaatgagtat gactgtataa tattatctga
    33001 tataggttct aatacgttct tattatctaa tagtactttt atagattaca atagcaatcc
    33061 agatagactt gaactaatca aagagtatgt aaataatggt ggggcattaa tcatggtagg
    33121 tggatatatg tcatttacag gaatagatgc aaaagctaga tttggagaaa ctgctatcaa
    33181 agatgtatta ccaataacaa tgatagataa agatgataga gttgaaaaac cagcaggaat
    33241 aattccagaa gttgttgact ctgaacatcc agtattaaaa ggtataccaa cagaatggcc
    33301 gaagttttta ggatataaca aaacagttgc tagagataat tgtcctgtgt tagcaactat
    33361 tggaggagac ccatttgtag ctgttggtga gtttgggaaa gggaaatctg ctatctttag
    33421 ttctgattgt gcacctcatt ggggtccaaa agaatttact gattggaaat actataataa
    33481 attatgggtt aatatgcttg actggttaac atgttaaaaa ttttataaaa cttgaaaacc
    33541 tcttacttga taaaatgaaa tttacaagtt aagaggtttt attgcataaa atggctgtaa
    33601 agtagaaaat atttttaaat cgttaaaatt agttattttg gctaatgaat ggataagtta
    33661 ttagaaataa ggaggtttat acaattaata tgagtgattt aaataaaaaa tttttaatag
    33721 aatatataga tgagatacag gaagagttga gagatatatc agataaaata tggaaaaatc
    33781 cagaacttca gtataaagaa tattatgctt caaacttaca aaaagagtat ctaaaaaaac
    33841 atgattttag tatagatgaa ttagagggta tggatacagc atttgtggct agttatggag
    33901 aaggtgaacc agttatagca gttttgggag aatatgatgc gttagatggt ttgtctcaaa
    33961 aagtatccat tactaaagaa cctattttag atagtggtgc gggtcatgga tgtggacata
    34021 atttacttgg tacaggttca ataggggctg taattgcagt aaaggaatta atcaaactaa
    34081 aaaaattgaa aggaactata aaatactatg gatgtccagc agaagaggat ttgtcaggaa
    34141 aagtactgat gataaaaaaa ggtttttttg atggaattga ttgtgctttt agttggcatc
    34201 catttgatat aaatactcca ataagaatcc caacacttgc taactattct gtgaaattta
    34261 gatacaatgg gattagtgcc catgcagcac aagctcctta taatggaaga agtgcacttg
    34321 atgcagtgga acttatgaac ataggttgta attatttgag agagcatata tttgatagct
    34381 gtagagtaca ttatgtaact actaatggtg ggaaaatgcc aaatatagtt ccagattttg
    34441 cagaagtatg gtattacata agaggaatta aaatggagca tgtaagagat gtatttgaca
    34501 gaattgttga tatagcaaaa ggagcatcat taatgactgg tactagtatg gaatatgaca
    34561 ttataagtgg agtatatgat tatattccca atacaatatt aaccgatatc ctatcacaaa
    34621 atatgaaatt gataggagtg caaaactata atgaagatga ttaccatttt gcagaaaaat
    34681 tagctgaaac ggtgtctata gataaaagaa ttagtgtttc tagtgtgtta agtggaaata
    34741 aggatattac taagatgaat ttacatggtg aagttacaga tgatactttt actcataata
    34801 actgtatatc attatcattg gatattggag atgttagtta cataattcct acagcacaat
    34861 gttcttgttc ggtttggcct attggtattt ctgctcatac atggcagtct tgtgccagtg
    34921 caggttctga tatgggattt aaagctatgt tgttagcatc taaatctata gcatgttcaa
    34981 taaatgatgt gatgcttgat gaggtgatta taaataaagc aaaaaaagaa ttgaagaata
    35041 ctataggtag ttttgaatat ataccaatta tttaaaactt aaattgataa tataagcatg
    35101 aacaacttat ataagtatat aatttaatat tatttttaac ttaaccttct aattatttta
    35161 aatttaagat acaatgatta gaaggaattt tttaagtttt ttagaatact gtatcttata
    35221 taatcttgat aaaaagtgtt atactatata taatctaaat aaaaaatagt acaaaaagga
    35281 gtatttatgt ttggatatgt taaaataaac aaaatggatt taacatttag agaatatgac
    35341 tattacaagg cttactattg tggactatgt aaatacttaa aaagaaatca tggagaaatc
    35401 tctaggtttt ctttgaatta tgatataaca tttttaattg ttttattaac agcagtatat
    35461 aacccagagt ctataagcac agaagaagta tgtatagtaa atccatttaa gaagaaaaag
    35521 gtaattacaa atgatattac agaatatgca gcaagtatga atattctttt aacgtattat
    35581 aaattagaag ataatttaat ggatgataga cgcataaaag ataaactggc atattatata
    35641 tataaaaata aattaaaatt agcatatgaa aagtatcctg aaaaagcaga gtatataaaa
    35701 caacaattaa atgaattaaa taaattagaa aaagacaaaa atataaatat agatgaagta
    35761 tctagtatat ttggaaatat aatgggtgaa gtatttgcct ataaaaaaga tgaaaatgag
    35821 agaaacttaa gaatgatagg ttttaatata ggtaagtata tatatctatt agatgcctat
    35881 gaagatttag atgaagattt taagaaagga agatataatc catttataga gtatattgat
    35941 aaaaatgatg agttaaaaga aaaagtaaaa aaaataatag tgacatcatt agggttttta
    36001 gctaggggaa ttgataatct aaatctaaat aaaaatgttg gaataataga aaatataatt
    36061 tattctggtg tttatttaag atatatgaat atattagaaa gcagaggtgg aaagaatgta
    36121 cagtaatact tataataatg aacattttat gagagcaaga gccttcatag aaaataatga
    36181 atttaaaaaa gcatatgatt ttttaaaaac tttgactgac aaatgtgcag agtggtatta
    36241 tttaactggt ttttctgcaa tgaatatagg atattatgaa gaaggtgaag actttttaaa
    36301 aagagcaaaa tttatggaac ctgaaaatag tgaatatagt gatgcactta gaagttatac
    36361 acaatataga aatgattata gcaatagggc agacaattat aatagaagaa gacgtaatga
    36421 tttagatgga tgttgttgct gttgctgtga tgattgctgt tgttgcctag gtgacgattg
    36481 ttgtgaaaat tgtgctaaac tttggtgttt agattcatgt tgtgaatgtt ttggtggaga
    36541 tttaataact tgttgttagg atgataaata tgagtataaa agttgcttat ggaggagttt
    36601 tattagcatt aaatgtaata ttgttgactc ttacaaatat tattcctgta aatacattgt
    36661 ttattatggg gttagcatca cttttagtat caattgtaat aatggaatgg ggctttaaga
    36721 gtggaattgc tttttatata ggttctatag ctttaggatt tatagtgatg gcaagtaagt
    36781 ctcaatggat tgtatattca cttacctttg gaatatatgg tattgtaaaa tatcttatag
    36841 aaaaagatag gtctatatat attgagtatt ttatgaaatt agtatttgca aatataatga
    36901 tacttatatt gtatttttta ttaagaacaa tagtttatat tccaataaac atatttatca
    36961 taggttcatt tgagatagcc tttatagtat atgactatgt tttcagttct tttataggtt
    37021 attacaataa taggttaaga aagatgctat ttaaaaaata atagttgatt tttatattta
    37081 attttaatgg aattgtagtt tggataagtg taaatgatat atctgaatat acattaaaac
    37141 ataaagctaa atttaatata ttcttagtgt aactaataaa agctttgatt tttagtcaaa
    37201 gcttttattt attaaatata aatattactt ataatataag tctttaaaat agatagtata
    37261 aattaaatat ccactatatt tacatctgta gataaagaat aaataatatc ttttctttct
    37321 actattattt caacattttt agaaatttcc tcatcaagtg atatttgaga caaaagctcc
    37381 tttgttggat ttattgctat tggatttcca actctcttta acatatttat atctccattg
    37441 gtatctccat aggcatacga gtttgataaa tctaagtcgt actttacaac aaaatcattt
    37501 atagcattat tcttacttat agaatcccac attggaataa ctgtgcctgt aaatatatta
    37561 ttttcaaaga cataatcact tcctagaaaa tcggttacat tatatttttt tgccattttt
    37621 tcaacaagaa atccagggct accagatata aatataacta tatgtccttg atttagatgc
    37681 cactcaatcc ttgaacgcgt gtatttataa actctatctg cttttaattt aattacttgg
    37741 tcacttgtaa agttgataca ggttttatct aatcctttta atgaatcaac ataaaggtca
    37801 catatttcta atagataatc atcataatta ccctgtcttt tatcccaatc cataaaagta
    37861 tctctagcat gtttatacca atttttttgg tcaattatat cgtatttaat taatttctta
    37921 aaatgttcaa ccataagaga gtctctataa agagtaccat ctatatcaaa aaaagctgct
    37981 atatttttca taagaccacc tcaattaatc atttccttat attatattat aaattgtatc
    38041 taagttgtta aaatatatgt ataataattt atatattaaa aaacaagtat tttaaagttt
    38101 aaaggatgtg actatatgaa ttttaatttt gcaaaggcta caaattctag acttatgggc
    38161 agtttaggtc ttatgataaa ttggatagat gatgaaaaca atcacttttg tcaatatttt
    38221 ttattagatg cagagggatt aggattagca gattatgtga gtttaaaaaa tccgacgcaa
    38281 gaagaagctt atatggaaga agaaaggttg atgggaggat ttggctcaga tagagttgaa
    38341 ttaacaaagg atgaatcttt atttttagta tcttattttg gtaataagaa tctttattat
    38401 aataagctac tgccaggaga taaatgtgaa tatattgata ttataaaaaa ctataaaaca
    38461 gatttaacca tagaaaaatt atatgataaa atatgtaaaa gagttgatga ggaagttgaa
    38521 tttataaact atatgactat gagatttata gcatgggata gagaaagttt aaagtacttt
    38581 tctggaagtg acgagattgc aaatatgcat attaccaata taaatggaac attacttaaa
    38641 aatatagtaa gtgataaagg tcaaggcaga tatataagtg aagctttata tgaagacaat
    38701 gatggatatt atatatgtaa aattgctttt tgtataagta aatgttatga aactggtttt
    38761 agaattaatt ctctattagt aacagataaa gaacctatgt atgattttga ggtatttgat
    38821 gaaatttcta aaagcgaatt tgtaagtgta tacagtgtaa attcgcctga agagtttgca
    38881 ggagtatttt atagagaaaa tccatttctt ttaaaaagtg atatgaatga aggtatattt
    38941 tttactagat ttaattttaa taatgaccat gtaaaagaaa atgtatatgt aataaataat
    39001 gatatgaaag ctatatatta cttaatggga aataagtttt ttattggtac ttatgacgaa
    39061 aatgattgta attatatcaa tgaaataagt cagtctaatt atagtgacta tattaaattt
    39121 gaagagaatt ttttctttga gcaaaatgca ttatatgatt ttgcagaatc aggttcatta
    39181 gattttgatg actttttgga ataataaatt taggcattat ttaagcgaga ttaactgaat
    39241 ttaatctcgc ttaaatttat ttctataata ttgggggaat attatgagga atacatttat
    39301 tattgtggtc aattattaaa attttataca gtttattaga aaaaatttat aattttcttt
    39361 atgttgtttg ctaatattag tctaaaatat gagtatcata ccattcttaa ctacaaacaa
    39421 aagaaagagt ttattaaatg aaaaagtcag ataaaattta attttatctg actttttcta
    39481 tatttatctt attagattat gttaatattt gttgaccgaa taagcgtata ttcagtcaac
    39541 tttctatacc tataataata ttgggggaat attatgagga atacatttat tattatggtc
    39601 aattattaat gttttataca gtttgcttaa aaaaatatat attacatttc ttcctcatca
    39661 aattcctcaa tattagtagc ctgtggtata ccttgcatag acatattact agaaccataa
    39721 ggagtaggat atatattagg aggcatagta ctaggcataa ctacaggcat accttgcatt
    39781 gtattatggc atataacacc tggtatcatc ataagtacat taggaggcat attttgatta
    39841 catgagaagt taggaatacc catagtatta ttcatattat accccatatt actgttcatg
    39901 ttattaggca tacctgtatt accatacata ttattattca tattgccata catattgtta
    39961 ggaataccca tattactatt catattatta ggcatattgt taggaatacc catattacta
    40021 ttcatattat taggcatatt gttaggaata cccatattac tattcatatt attaggcata
    40081 ttgttaggaa tacccatatt actattcatg ttattaggca tattgttagg aatacccata
    40141 ttactattca tgttattagg catattgtta ggaataccca tattactatt catgttatta
    40201 gacatattgt taggcgtacc catattacta ttcatgttat taggcatatt atttgtagca
    40261 gatgtattgt aattcatatt tccagatgta ttatttgtgt taggcaagtt gtttggagaa
    40321 ttactacttt taggataatt atttatgtta ttaggatata ggttatttat gttctggtta
    40381 ttaggtactt ttgtataacc attagaaatt tgagggtcat ttctcaattc accttgggga
    40441 atttcttcat ttgtaatata atctgcatag tttgctgggt ctaagtaagg catgtaacta
    40501 gtatctaaat cctttttttt cttgttactc atattaaact tgcacctcct agaaaactat
    40561 aaaaaagttt acaaaaactt attctaataa tatatatatt aaaaaagttt ataatatgtg
    40621 cattctaatt gtattaaatt aataaaaatt attgttgcaa atttagaaat gatagcatat
    40681 aatgaatata tgaacagtta ttcatatatt aaaataacgg aggattaaaa aatgagagaa
    40741 gagataaatg attgtaattg taatataata catgaagaaa tagtaacaga ggctaaatca
    40801 actatgccag atgaagagat gttatatgat ttagcagaat tatttaaagt atttggggat
    40861 actactagag taaagatatt atatgcatta tttgcaaatg aaatgtgtgt atgcgatata
    40921 gcgagtttat taaatatgac tcactctgca atatctcatc aacttagagt attaaagcag
    40981 gctaggttag taaaatttag aagagaagga aaaacagtgt attattcatt agatgatagt
    41041 catataagtc agatatttga ttgtggatta aatcatatta gagagactta taaatgataa
    41101 atgagaggag tgaatatata tggaagcaaa taatttaatt aaaaaagaat ttatactagg
    41161 aggattaaat tgtgctcact gtgcagaaga gattaatgat aaagtttcta aactacaaga
    41221 ggttaaatct tccaacttaa attttataaa taaaaaactt acagtaaata taaaggaaag
    41281 ctttaatgag gatgctacta tagaaaagat aatagatata atagattcaa cagagccagg
    41341 acttgatata caaataaatt ctaaagaaaa tgcatctaat aaaacatcaa ttaaaaaaga
    41401 gcttatatta ggaggattaa actgtgcaca ttgtgcagag gagattaata acaaagtttc
    41461 taaattaaaa gaagttgaat cttctaattt aaactttgta aataaaaaac ttacaataaa
    41521 tataagcaat aactttgaag aggatgatgt tataaacaaa ataaaagaaa taataaattc
    41581 aacagagcca ggacttgata tacaagtatg gtctaatgac aaagtaaaag agagaactac
    41641 tgaaaaatca ggagttgcaa atgatacaaa taagaaagaa ttaatttcac ttataattgg
    41701 agttcttgta tatatatttg gtatatatca aacggctaca ggttatgaaa gtcagtttag
    41761 taatatagta tttatagttg cctatgtgat aataggtggt gatgtagttt taagagcaat
    41821 aagaaatata tcaaaaggaa gagtatttga tgagaacttt ttgatggctc tagcaacagt
    41881 aggagcatta gcaataggag aattatcaga agcagtagga gttatgcttt tctacaaagt
    41941 aggagagtat ttacaaggtg ttgcagttgg taaatctaga aaatctataa cttcacttat
    42001 gcaaataaga ccagattatg caaatctaaa agttaattct gaagttaagg tggtgtcacc
    42061 agaagaagta aaggtaggcg atataatagt agtaaaacct ggtgaaaaag taccattaga
    42121 tggtgtagta gtggatggag tttctatgct agatacttct gcacttacag gagagtcagt
    42181 gctgagagaa gttgaaaaag gagatgaaat tttatcaggt gttataaata aaaatgcttt
    42241 attgagtatt gaagttacaa agagttttgg agaatcaaca gtttctaaga ttcttgacct
    42301 tgttgaaaat tctagcatta aaaaatcaaa gacagaaaac tttatatcta agttttccag
    42361 atactatact ccgatagtag taatagctgc tttactaata gcatttatac caccacttgt
    42421 aatcccaggt gaggtattta gtgattggtt atatagagga ttgatatttt tagtcgtttc
    42481 ttgcccttgt gcattagttt tatcaatacc acttagtttc tttagtggaa ttgggtttgc
    42541 atctaaaaat ggtatcctta ttaaaggaag taactattta gaagctttaa gaagtgtaga
    42601 cacagtagta tttgataaaa caggaacact tactaaaggt gtgttcaatg taactaagtt
    42661 aaatcctgaa ggtatatcag aagaagagtt attagaatat gcagctattg cggaggtaaa
    42721 ttcaaatcat ccaatagcaa agtctatatt aagttactat aataaaaaaa ttgatttaga
    42781 tacaatagac agctatgagg aaatagcagc ttatggaatt agagtaaagc ataatggcaa
    42841 gtttatatta gcaggtaatg agaaacttat gaaaaaagaa aatatatctt attcatcagc
    42901 caaagaagta gggacagtag tctatattgc tgttgataaa gtatatagag gttatatagt
    42961 tatatctgat gaagtgaaag aagatagtaa aaatgctata aaaagcttaa aagcaatagg
    43021 agtaaaagaa gttgtaatgt taactggaga taatgagaaa gtagcaaaaa atatagctcg
    43081 agaattagaa ttagatacag tttactcaaa tttacttcct aatgaaaagg tggatagatt
    43141 agaagagtta tatgaaggaa gaactgaaag agaaaaaata gcatttgtag gtgatggaat
    43201 aaatgatgct ccagtattag ctcgtgctga tgttggtata gctatgggag gtttaggttc
    43261 agatgctgct attgaagctg cagatgtagt gcttatgaca gatgagccta gcaagatatc
    43321 taaagcaatt gaaatagcta acaagactaa taaaatagtg tggcaaaaca taatatttgc
    43381 tttaggagta aaaatgatag ttatgatatt aggagctgga ggagtagcca ctatgtggga
    43441 agctatattt gctgatgtag gtgtgtcact tatagcagta gtaaatgcta tgagagctat
    43501 gagataagga ataaatgtaa atctatacta ttcaaaaaac ttataattat atattgaagt
    43561 atacaatgat gaaaggtatc aataagtttc ttttaacttt attgatacct ttttattgga
    43621 gtacttttgt atatataaat ctatattcat ttataaattt gtattcatta tgtttattta
    43681 atgcatttat ttagtatata acttttgtat tgtaatttta aaatttagat atagaggatt
    43741 tattgataaa tttatattaa gtttaaacta gactcataaa aattataata cttacgattg
    43801 catatatatt atattaatag ttataatttt tacaataaaa tgtgtgtgct aagatactct
    43861 aaatcaacaa tataactgcc attcaataaa ttatgtaata taataaagtg aaaaatttaa
    43921 aaaaataatt taaaaaagtg ttgacg
//
LOCUS       DAMJVM010000024        42195 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.024, whole genome shotgun sequence.
ACCESSION   DAMJVM010000024 DAMJVM010000000
VERSION     DAMJVM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42195
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.024"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            274..834
                     /locus_tag="PC523_001397"
     CDS             274..834
                     /locus_tag="PC523_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2474933.1"
                     /translation="MTNILKFKLLSFSKEKKEHSLEKKAIYGDKKAFENLIENNEEYL
                     YKMAFLYTKNEHDALDIFQDTVYKAFLNIDKLKEPSYFKTWITKILINTFNTKYKKTV
                     NYLNIRDYEHKIEEVNYQQIEDKIDLYDAIDRLDEKYKTPIILQYFQDMTIGEIAEIL
                     DINENTIKTYLRRGKASLYELLKEEK"
     gene            836..1870
                     /locus_tag="PC523_001398"
     CDS             836..1870
                     /locus_tag="PC523_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4179 domain-containing protein"
                     /protein_id="HDF2474934.1"
                     /translation="MSKYDDIKIPESLKRVRNSAIEKAYKDKKFKRDKKRFTMTVSIA
                     IIGLGLLGIGVSNPTFAQKILRVNEMIQAIQGIFTNNELSDSITTIGESSTRDGLTIT
                     VQNAYYSQNEITLEIELKTDKPFKNTKYSKVIVKDNKDESIDDFALMTKDFNTWINGE
                     ETKGIVYESPRFKYIDEYTLKGYWQINFKSPIENHKNYDTIKMSFGLNETNYDKKSNN
                     YKYTNSINGPWILEFPVKSNMDKVKIIKPDIRQDIITVKQVALSQTSLNLKLELEKQG
                     VIEYVEVKDDNGETLGGGMESSNPNKIDLDFGLKNVNQSPKYITIFAYGNIDNGKDES
                     KAIKIKINLE"
     gene            complement(3077..4108)
                     /locus_tag="PC523_001399"
     CDS             complement(3077..4108)
                     /locus_tag="PC523_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474935.1"
                     /translation="MKIEMGESLLYSWLRHVKECQIVQTNWKVSSKWDLKNADKILEI
                     MEKTKQYFEQKYGYPIYKNNSLEQLIMQAEVDVLGISIDNGRNHIYAIDVAFHEAGLN
                     YGSKNETVTRVIKKCIRTAMCIYGYMDLDEAEIIFASPKINPAIITELEPKMAEVNDL
                     FKSLGLRFKTRLIANEEFNGKILEPILIASDGISDTSELFLRSYQMYSMFSYSRDVVK
                     QQRIKKSNSLKDAVELNLSDETLKELKIGKLVQVTMKHLFEEEKISPEEIDKMTQSSY
                     SKQVFNENKPVLKEVPKDGNINELKKDEKNYNRYYAYTISIYGKQYLLVSQWIEDLHR
                     SYYESWVIK"
     gene            complement(4259..4909)
                     /locus_tag="PC523_001400"
     CDS             complement(4259..4909)
                     /locus_tag="PC523_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474936.1"
                     /translation="MEVIRNMTNVSSLEKEDEIVEILSSKENIRVAIEIERGLKLCKT
                     QMIKKVLEEIEKRMDKKFEDKYKLPYYSYKENNYALVNNYYNKKSSTYPAINYFIKSL
                     DKEDVDLLLRIEIDHHIFVGFCTLYKEKPSGKILSDDEIKELINDDGSRTNGWWICWE
                     YIYNNTMECPNFKNFNDAYFDLFDDNKFDEFMDLCEKRILSILGKLKDKQCINTFI"
     gene            5102..5560
                     /locus_tag="PC523_001401"
     CDS             5102..5560
                     /locus_tag="PC523_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HDF2474937.1"
                     /translation="MSVDTLRNQFIKIVIEIYEDLTEFIFSKDIQTDKNLDFILKEPK
                     YIPNGFKEVEKENTLQGIFIRYENKNGSEIRYRSNQIDSNTVILDTENAKVTDIMVND
                     YEAKYIVKDNSITIFWNDEENVYTIYFDYDNKNDLNKYKDNLIDIAENIK"
     gene            5635..6057
                     /locus_tag="PC523_001402"
     CDS             5635..6057
                     /locus_tag="PC523_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474938.1"
                     /translation="MYLKKVIVAGLCTLTCLLPLNVQAFEDTSLNTNDYNQTNDNDLF
                     MIYIKGAGSNLSIRSGKAHISCYVDGKIGSLTSITARLQQKKSGKWVTISTWSSSGKT
                     SSTLSREYRVSKGYSYRILTTIKAGNESRNITSNVTNY"
     gene            6087..6773
                     /locus_tag="PC523_001403"
     CDS             6087..6773
                     /locus_tag="PC523_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474939.1"
                     /translation="MKYFKKVIALGLSIALLIPVISINALEKKDTNVNNQIINVIESN
                     VSYTKEGIEVSSDLKNDLKEINLEDIKEQMIEQGIDSKFINNFNSDVIYSLVKENIDD
                     INEGVKNKTLKITPEEDIINNTDNSFYVQGGSTYTQKFSWGVRQNKSTAAANRWVYDL
                     NKSSQGFVATGAVSVLLGSMGAPIGFVSGLSAAYMGGLANKVSYVNSRNNRGITVDIN
                     WWLTYAVKNQ"
     gene            6921..7733
                     /locus_tag="PC523_001404"
     CDS             6921..7733
                     /locus_tag="PC523_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HDF2474940.1"
                     /translation="MNYSDMHEISSGKSQKIALIDSGISKFQKVYKNINLTNSNNDYD
                     TNGHGTMMYSIIKGYNKNNQTIEGISPDSRIILIKVMNDDESINPNVIEDAIDYALNE
                     KVDIISLSLGSTKTDINIKQKIDKANLKNITVVSSAGDYKQGFLLFPASLNNVISVGS
                     IDDRRDISTLTNAPQKTLINAPGEKIKVIDNNNNVFYGKGTSEATAIITGYIALMRDT
                     NISSTQILTNEKIMNLLSQINKNEKTYLGALKEIRKKVSVSTGAFFSFDLSY"
     gene            8315..8476
                     /locus_tag="PC523_001405"
     CDS             8315..8476
                     /locus_tag="PC523_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474941.1"
                     /translation="MIDNKYIFRGKNIKNYIKFKDITGANKQDSLIYLKALSIEREEY
                     LINRLKSFF"
     gene            complement(8678..9034)
                     /locus_tag="PC523_001406"
     CDS             complement(8678..9034)
                     /locus_tag="PC523_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HDF2474942.1"
                     /translation="MNIIYEKEFTFDNKVREFISLGNNYKFEFSTSKSPKTLIKYFMD
                     YNKISSNKLESFKEKYSFKKNELLSLQEILKNKFTEDDKLESLKLSKANLENELKLGN
                     SIIKKSNSNHNMVEKK"
     gene            complement(9150..9380)
                     /locus_tag="PC523_001407"
     CDS             complement(9150..9380)
                     /locus_tag="PC523_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2474943.1"
                     /translation="MSYAETKACACSDDSILKLCEINREIQIIEINKKAFLNQNLLTL
                     NDIKSTENSIDALKISISKLEEDIKTYFSNFF"
     gene            complement(9553..11766)
                     /locus_tag="PC523_001408"
     CDS             complement(9553..11766)
                     /locus_tag="PC523_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF2474944.1"
                     /translation="MNQSYHNIKNELLSKQLAYIDRLTGYLVDRDNYLSGYVKEKLKI
                     AKEASIKDNTFLVNVKALEANQPKYETDVFFSIDSTWISSSIKSDFIEKTLELKENSI
                     QLLYSTQLGYSVKCSDIIPISKNEFTWGTKRRKSLYIVEAELNMKTITVTDKEYQNGK
                     EVSVKNLEETQLAMNILNKWLIAFHNYVTSNSYIHKELLDRYNEMFIDYKEKEYKNIL
                     NDVRINPNIKLREHQLKAASRIITSDRNTLLCHSVGSGKTYTMITAAQELNRISKIDS
                     KKGNKNLFVIPNSLCESGQFAKEYFNLYPQANILATTSKDFSKSNRRKIIAKIVTCNW
                     DSIIIPHSVLGLIPLEADTELKLLDLYLKELNDTLKYYKKECPDLSTKRLETMKENYL
                     NRIKELNDMHKDDALLSFEKLGITNLFIDEAHNFKNLYFNTTMQVPGISTLKTKKTQD
                     LYNKLRYFKSIHGNKGVTFATATPISNSICEMYTLLTYLNKDTLKYYKVDTFNAWASK
                     FGSIISSMEVDPTGQGFRFQQRFAKFCNVPELVNIFRQIADVVNICDINDLDIPQIKG
                     GKPIIEIIKPTNQMKEYIDSLVKRAEKIHEGPYDPIIDNMLNVTTDGRLMAVSPALVG
                     IDGILPKIIKVGENIASLYHEDKISTHLVFCDLGTPNGNSHNVYNDIKNELLKNNVLE
                     KDIAYIHDANTPEKRKKLIDDFNNGIIRILIGSTSKITSSCRCSLETFINFTKRR"
     gene            complement(12262..12828)
                     /locus_tag="PC523_001409"
     CDS             complement(12262..12828)
                     /locus_tag="PC523_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474945.1"
                     /translation="MNNLFYDKLNFDDKLIIKNNGVIRNNDLYFLKYKNDTAGILIYN
                     IINDKFSFKQNKNYKGMNPFPITNIFKNLDSDKWDEFIRIWIEERVIQECRPEVPFIL
                     KEFKLGYYDVWDICRSNLAMSVEDFFWITKDNTLRYEDFNIRYCTLNNITPIYKPPFP
                     IVIYPNYFEREKIKSGETWKYELKNTNS"
     gene            complement(13230..14282)
                     /locus_tag="PC523_001410"
     CDS             complement(13230..14282)
                     /locus_tag="PC523_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2474946.1"
                     /translation="MNVINVFNINLKRNINKRIVFLVTLLFPIIIVILGVLANYISKP
                     FFNIGILDEYKNERVLKESEKRTYENTIMALENTQGINTKIADFKTIKTDIITGKYSA
                     VIYFNKNGFKLYSIKNKNTNDTLKYLIETYKKDPVPVDISKLQENTLGVLQRIIAFIV
                     LFLMITSTVTASMIIKDKNSGTFTRVLYSPQNVRGYVLGNMLYNFTITYFQYIVSISI
                     IKLFNIDIGMDYIDLILMGIWVVALATSFGTFVSSLFNREMHANLFSTCISLVLSLIG
                     GSFISVEKMPEMLQKISIISPVRWFISFVTYIEERDGSFFNMNYICILTSIIIVLALL
                     AMKFTQRSKYSSNSNY"
     gene            complement(14282..15022)
                     /locus_tag="PC523_001411"
     CDS             complement(14282..15022)
                     /locus_tag="PC523_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2474947.1"
                     /translation="MRCFLPLFKLGIKRRSKDFFILFYNIVFPVIIIMLLGYLTSKSY
                     GEEFTSYNYYTIVTIPFCVLMGIISVSYAGQDEKISNTSYRYMISPISIVDLVISKLF
                     SCTAVLSICNLVTLVVLKCIFRMSFGGKFLAVLFFLMCESIVVVSMGLFLGLVCKNLD
                     MLRNIINIPIVIFGFLGGVFFPVSSLNPVISLIINISPLTWINRGIVACIYDNNLQLL
                     FNISCIFVVVSIFMTILTIKFFKKGVFI"
     gene            complement(15027..15785)
                     /locus_tag="PC523_001412"
     CDS             complement(15027..15785)
                     /locus_tag="PC523_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2474948.1"
                     /translation="MSNILLEIKNLSKKYNNKEVVSIESFKLLEGDILGFIGANGAGK
                     STSINMLTTIVSPDSGSIYYKGKDIKNQQNIFKSSLGVVPQELAIYEDLSAYDNVKFF
                     CSLYGFKGNELKERTKDALEFVGLWESHKEFPSKFSGGMKRRLNIACAIAHSPKILIM
                     DEPTVGIDPQSRNKILEAIKCINTNGTTVIYTSHYMEEIETLCNRIALIDKGIIIEDL
                     DKSLYKNKYLEFGCKTLEDIFLYLTGTDLRDLEE"
     gene            complement(15887..16609)
                     /locus_tag="PC523_001413"
     CDS             complement(15887..16609)
                     /locus_tag="PC523_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2474949.1"
                     /translation="MIRTLILYYLNIKPTHGYEIQKFLQVSGADRWTKIQSGSIYYAL
                     AKLEKDGGVRVLKEEKTGARIRKIYEITQSGKLELREEIQKELQMPIVPTGSNKFLLH
                     NILDVLPKDTLQKNLEKHIKYLIEQKKYWENWKEIKKIDKKSLATEKIAFDMTIDNLN
                     YQILWHEEILNDIDKYISVGCETQNIIKSIDFSNIEEDFLFTSDTTNELLEVQRLRDE
                     IINNPDKAIENIDKIILKLQNK"
     gene            complement(16710..17315)
                     /locus_tag="PC523_001414"
     CDS             complement(16710..17315)
                     /locus_tag="PC523_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2474950.1"
                     /translation="MKIKENVLRLDSTRGSNCFIVFDKEITLIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHMSNIKLLKEKTGAKVWAHTEDIPFIIGEKDRPGFKK
                     FIGKTISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVSYPNPWNWDSKKMMKSIRELDNLEYEWICMSHGTPCIK"
     gene            17495..18133
                     /locus_tag="PC523_001415"
     CDS             17495..18133
                     /locus_tag="PC523_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2474951.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFSLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSKKSDTEMLFKEKFFSFNKFMFDEIQEMFEIGKNDGSIRYDI
                     EISTLMYSSIFTLTGFFNLLSVTRKSYLNNFNIDEEKFIETTLAILIDSIKA"
     gene            18249..18911
                     /locus_tag="PC523_001416"
     CDS             18249..18911
                     /locus_tag="PC523_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2474952.1"
                     /translation="MAKILLLEDDDTITFGIKTSLENKNHIVCCYNTIAEAKKNFDKT
                     TDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRAHSNTNNTITCGNISLNKVEVKAYLDSVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            18911..20092
                     /locus_tag="PC523_001417"
     CDS             18911..20092
                     /locus_tag="PC523_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2474953.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKVL
                     DASYEYNPKSTESILKSLKNSSDEDFKTGNEILSKHGYSKGTFWSKNFFYFLVVSFGI
                     ISTMAIVVYIIKYRTNKQKLSRINMLTRYLEEVNLGKESVLLRYEDEFSYLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TKQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     KGSPSIEFEIDINWTLEALLNILKNCSEHIEAGGHIIAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(20269..22929)
                     /locus_tag="PC523_001418"
     CDS             complement(20269..22929)
                     /locus_tag="PC523_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2474954.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDDTYGKDLSLIENFSSVDDVMVKGQWYVGKINNDKRDYI
                     VYRNATSEYWNHMPEKDSIIEGRRPSNTNEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPVAPKSKGESFKKISKTTLTIVGKVDVATSSVVPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SNISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNKLIVYINSVNYHTDAPEVVFTYGIPAFLPAVLL
                     TIVTVWISTLIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTGFLHIIANQEGAEAVYGSDNYKDQRDIEVYLENSEPVE
                     SDFIEKLDNTEGVKNSLYYQKMSATTWLTEKDASKELSKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDESFAEYCRQISVDPKLFYDTKNPTSIVYNKEEDVNRSTRRNKVY
                     IDYLNLSIGDNIKLNEKIYDEDKGDYTYNMKVGAITSTLPKIIDTSSRYTLMQIVPIS
                     MVQEISKNYAEVGRSRSNRLIGLFKVDNREDIPRVRGKLESICNKDYGSGDYSIEDVV
                     ENEKQNGSGRKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPD
                     GIKKMLVLEGLFLGITPLLLSIPVQIGIVYTFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYNQLKNENIVESIKSETI"
     gene            complement(22926..23609)
                     /locus_tag="PC523_001419"
     CDS             complement(22926..23609)
                     /locus_tag="PC523_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2474955.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHMLGGVDRPTSGKIYVEDTEISSLNEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     TILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            complement(23958..24890)
                     /gene="blaCDD"
                     /locus_tag="PC523_001420"
     CDS             complement(23958..24890)
                     /gene="blaCDD"
                     /locus_tag="PC523_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HDF2474956.1"
                     /translation="MKRKKNFIWTTILLVGIVMVMYYAGKKHNDINQKTDKNYIKSDL
                     NKSEINNDNNKKERVNIVNYSDCFEGISGGAIFYNSRNQEYNIYNKDLIETRASPCST
                     FKIISTLIGLEKGVITSKKSVIGYDGTNYPNKNWNKNLSLEEAFKESCVWYYKKLINK
                     VDAKSVQSILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMVKIF
                     EGDTNFKKEHINILKDIMKIDVSDKNINVYGKTGTGFNGKNKRVDAWFVGMLESKDDT
                     YYFAIKSDDSNKEIAGPKVKEIAINIIKKYYSVM"
     gene            complement(25430..27544)
                     /locus_tag="PC523_001421"
     CDS             complement(25430..27544)
                     /locus_tag="PC523_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HDF2474957.1"
                     /translation="MFEDKQVINCDTIASNSKHNMYEHDTELLTFFPDEIAHLIDINN
                     KLDDAFKKAENLVDKLDKDYMDTKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKIDKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKAHTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     AERVRFKSTFDFVKLIDYYIKQMPNKIFIPKDYNFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKLKSILKSLNGMLTIKNTLTLYKDFFKQMNLSN
                     MFVMATKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHVVIDEMQDYTPIQYAVINLLFK
                     CKKTILGGFGQLVNPNHTHTLDDMRQLYNEGELVTLNKSYRSTFEIINFAKKVQDVSS
                     LEPIERHGEEPALVKCTNKQDEVSKIKIEIEEFKKSDNATLGIILKTDSDAEVIYNAL
                     KQEYSVNLISSESSSFTKGVSITSIKMSKGLEFDEVIIPSVNSKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTGELTQLIDM"
     gene            complement(27791..28651)
                     /locus_tag="PC523_001422"
     CDS             complement(27791..28651)
                     /locus_tag="PC523_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2474958.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLANMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSEIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     SVEETDTRPN"
     gene            complement(28842..29456)
                     /locus_tag="PC523_001423"
     CDS             complement(28842..29456)
                     /locus_tag="PC523_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2474959.1"
                     /translation="MNEKWLEWAIELQSIAQAGITYGKDIYDRERYERIREISAEIIA
                     YKTDISTEKVRNLFCNESGYQTPKLDTRAAIFENGKILLVKENTGKWSLPGGWVEVNL
                     SVKENTIKEVKEEAGLDITADRIIAIQDRAKHNLPIYAYGVCKIFVLCTVIGGEFKEN
                     IETTEFSYFSENELPELATEKNTEEQIKMCFKACRTDTWTVIFD"
     gene            complement(29524..29886)
                     /locus_tag="PC523_001424"
     CDS             complement(29524..29886)
                     /locus_tag="PC523_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2474960.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDEALKPFVETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQE"
     gene            complement(30106..30570)
                     /locus_tag="PC523_001425"
     CDS             complement(30106..30570)
                     /locus_tag="PC523_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2474961.1"
                     /translation="MTRISNITIAEQSEYCILAIRKTIDFVVEFSEFSKQSLEKISKY
                     LEERGILSSGAPIVCFHNMDLAELDVEVGFPVATYIDGKNEILQRIIPSQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            complement(30880..32301)
                     /gene="nhaC"
                     /locus_tag="PC523_001426"
     CDS             complement(30880..32301)
                     /gene="nhaC"
                     /locus_tag="PC523_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDF2474962.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNVFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPVGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLKLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDI"
     gene            complement(32331..33395)
                     /locus_tag="PC523_001427"
     CDS             complement(32331..33395)
                     /locus_tag="PC523_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HDF2474963.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEVV
                     KAICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFYNVREIIKLKNIKIIGIATNLT
                     CYGAIIPSRENIGRLASIAQKMEGKFGINLEIVSGGNSSSIHLMTNDNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGFDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDSVEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            complement(33380..34789)
                     /locus_tag="PC523_001428"
     CDS             complement(33380..34789)
                     /locus_tag="PC523_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HDF2474964.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHNIDTNNPVFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDNIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQVTSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNIENQEEVRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKIKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            complement(34789..36993)
                     /locus_tag="PC523_001429"
     CDS             complement(34789..36993)
                     /locus_tag="PC523_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HDF2474965.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYR
                     DCTKPLKDSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMIRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLRIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELREKARELKERAVLYMEEIFEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDKNAIDNPSILIDGCTFENPDKIIYIDELDDFDNVENRLKESDEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQETEVISREVMHESEGTRIEVKGRV
                     PFDIDLKNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMNNK"
     gene            complement(36987..37361)
                     /locus_tag="PC523_001430"
     CDS             complement(36987..37361)
                     /locus_tag="PC523_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HDF2474966.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVNGEYWDEVTCLFREGVESC"
     gene            complement(37439..38869)
                     /locus_tag="PC523_001431"
     CDS             complement(37439..38869)
                     /locus_tag="PC523_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HDF2474967.1"
                     /translation="MNNSASMKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEVIQSQYAVGNTPIIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKKMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVIDVLNSL"
     gene            complement(38862..39170)
                     /locus_tag="PC523_001432"
     CDS             complement(38862..39170)
                     /locus_tag="PC523_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HDF2474968.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKVNPYYTHDYGKCIPELLQIGIQAKEILFGGVSNE
                     "
     gene            complement(39268..40335)
                     /locus_tag="PC523_001433"
     CDS             complement(39268..40335)
                     /locus_tag="PC523_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HDF2474969.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKILFLLNRKINVIS
                     TAEQMAYPQADDVDLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMVEQGVGATREEFIKGVEDKTIAGHVGFVESINMIADGLGWRL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            complement(40595..41968)
                     /locus_tag="PC523_001434"
     CDS             complement(40595..41968)
                     /locus_tag="PC523_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2474970.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSF
                     FDIVNKMKINRSTLLEVLEKREIIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIEKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     RVIGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESKNYSLNDLEDKTLSKIISEIEEKYIYESLEKTSYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
BASE COUNT        14291 a         6700 c         4913 g        16291 t
ORIGIN      
        1 atgtgaaatc gttaataaaa cttgtgggat ttgtatgatt attatcaaaa acatacctat
       61 aatcaaagag actatatgaa aactctcttt tttttataaa aacttatata taaataatgc
      121 aaatattaaa aagtaaataa atgttggaaa gattatgttt ctataagcta gtttctaaag
      181 agttattctg aaattagtta taaaatttat atttttgaaa ctttatatac aaaataatag
      241 tctaactaat gtgacaatat aaaggaggct gagatgacta atatattaaa atttaaatta
      301 ttatcctttt ctaaagaaaa aaaagaacat agcctagaaa aaaaagctat ttatggagat
      361 aaaaaagcat ttgaaaattt aatagaaaat aatgaagaat atttatataa aatggcattt
      421 ttatacacta aaaatgaaca cgatgcactt gatatatttc aagatacagt atataaagca
      481 tttttaaata tagacaagtt aaaagaacct agttatttta aaacatggat aactaaaata
      541 ttaataaata cattcaatac aaaatataaa aaaacagtta attatttaaa tattagggat
      601 tatgaacata agattgaaga ggtaaattat caacaaattg aagataaaat cgatttatac
      661 gatgcaattg ataggttaga tgaaaaatac aaaacaccta taatacttca atattttcaa
      721 gacatgacaa ttggtgaaat cgcagagata cttgatatta atgaaaatac gataaaaacg
      781 tacttaaggc gaggtaaagc aagtctttat gaactattaa aggaggaaaa gtgatatgag
      841 taaatacgat gatataaaaa tacctgaaag tcttaagaga gtaagaaatt cagcaataga
      901 aaaagcatat aaagataaaa agtttaaaag agataaaaaa agatttacta tgactgtaag
      961 tattgcaatt attggattag gattgcttgg aataggtgta tcaaatccga catttgctca
     1021 aaaaatactg agagtaaatg aaatgattca agcaattcaa ggtatattta ctaataatga
     1081 actgtcagat agtattacaa ctattggaga atcaagtaca agagatggac ttactataac
     1141 tgtccaaaat gcatattata gtcaaaatga aattacctta gaaatagaac taaaaacaga
     1201 taaaccattt aaaaacacta aatattctaa agtcatagta aaagacaata aagatgaaag
     1261 catagatgat tttgctctta tgactaagga ttttaatacc tggataaatg gagaggaaac
     1321 taaaggcatt gtatatgaat cacctaggtt taaatatata gatgaatata ctctgaaagg
     1381 atactggcaa ataaatttta aatcgccaat tgaaaatcat aaaaattatg atactatcaa
     1441 gatgtctttt gggcttaatg aaacaaatta tgataaaaaa tcaaataatt ataaatatac
     1501 aaattctata aatggacctt ggatattaga attccctgta aaatcaaata tggataaggt
     1561 taaaataata aaaccagata ttagacaaga tatcataact gttaagcagg tagctttaag
     1621 tcaaacatca ttaaacttaa agttagaatt agaaaaacaa ggagttatag aatatgtaga
     1681 agttaaagat gataatggag aaactttagg tggtggaatg gagagttcta accccaataa
     1741 aatagactta gattttggat taaaaaatgt aaatcaatct ccaaaatata taacaatatt
     1801 tgcctatggc aatattgata atggcaagga tgaaagtaaa gctataaaaa ttaaaattaa
     1861 tttagaataa tcaattatcc cttaaaagtt atttagaaat ttactaatta tagataaagt
     1921 taatgccctt gaaatccaaa taaatagatt ttaagggcat ttttattata tattttcttt
     1981 tatattacta aaagttacat atttatatgt attttatttt tatcaaatct atctataagt
     2041 ttatcttttc ttcgctgtat cctgtagata caaaggcata atacactagt ttcaaatatt
     2101 tcccccaatt ttctaaatat tttcactttt tcgttttatt actatgaagt ctatgatata
     2161 attttaaaaa gagatacttt ccaataaaaa agttatttta aacctattat taaaattttg
     2221 aaagttgttg atgattatga ataaaaaaaa tatattcaac gatatgtttc ttattgttta
     2281 tccaattata atattaattt ctatgttctt taactcatta ttacatgagc agatactaag
     2341 atttaatatt aataaacttc aatttatggt ttttatatta gtttattttt ttatatttgc
     2401 attgtttata tataagtttt ttataaaaaa agagagtttt catatagtct ctttgattat
     2461 aggtatgttt ttgataataa tcatacaaat cccacaagtt ttattaacga ttttacacaa
     2521 tttattcagt attgtttatg gacaatttca attagatata tttatgtttg gaacactttt
     2581 gactttatat acaataatta ttattatgta ttataaagaa aatcataaaa aaacaagaga
     2641 ttaatacctc ttatttttta tactatctta aagtttatat aaaaaacttt tatatttaat
     2701 aaccctggaa tagggagttt tggcaatcca atataaaatt gtatcaaaat taaggttttg
     2761 tgttataaca gtaaaagaaa gagaactata ttctatttag tgacagaatt gagttcgtaa
     2821 tttaaaataa aaaattattt aaattttttg aacttgattt ttaaatcgaa ttcctagcca
     2881 ctcttgccgg agtggctttt taaatgccta aataatttac ttaataagtt atattttgta
     2941 ttcttttaat ataaaaatgt gtgtaataga agatgaatac attaagttaa aaatgtttaa
     3001 tgtacaacta actattttat taaatttaac ttttatgtta ttgcattttt cttagattta
     3061 gcaagagcat tataatttat ttgataaccc aactttcata atatgaacga tgtaaatctt
     3121 ctatccattg tgatactaat aaatattgct ttccataaat ggaaatagtg taagcataat
     3181 atctattgta gtttttttca tcttttttca attcattaat attaccatct ttaggaactt
     3241 cttttagtac aggtttattt tcattaaaca cttgctttga ataactggat tgtgtcatct
     3301 tatcaatttc ttcagggcta attttttctt cttcaaaaag atgtttcata gttacttgaa
     3361 ctaatttccc aatctttaat tcctttaatg tttcatctga caaatttaat tcaactgcat
     3421 cttttaaaga attagatttt tttattcttt gttgttttac aacatctcga ctatatgaaa
     3481 acatactgta catttgataa cttcttagga aaagttctga tgtgtcagag attccatcac
     3541 tagcaattaa gattggttct aggattttac cattaaattc ttcattcgca attaatctag
     3601 ttttaaatct caatcctaaa gacttaaata ggtcattgac ttctgccatc ttaggttcta
     3661 attcagttat aatagctgga ttaatctttg gagatgcaaa tataatttct gcttcatcta
     3721 agtccatata accataaata cacattgctg tacggataca tttctttata acccttgtaa
     3781 cagtttcatt tttagatcca taattaagtc ctgcttcatg aaaagctaca tctatagcat
     3841 aaatatgatt tcttccatta tctattgaaa tacctaatac atcaacttca gcttgcataa
     3901 ttaattgctc caatgaatta tttttatata taggatatcc atatttctgc tcaaaatatt
     3961 gtttagtttt ttccataatt tctagtattt tatcagcatt ctttaaatcc catttagatg
     4021 acactttcca gtttgtttgc actatttggc attctttaac atgacgtagc caagaataca
     4081 acagtgattc tcccatttca atcttcatag aattaccatc cttttcagtc tattatctat
     4141 taattgttaa actatatcta aattcttcca tcatatcttc aaatcatgta atctcttatt
     4201 tgttatcaaa atccttgagt cttatacatt tacatacctt gttaggtaat attctatttt
     4261 agataaaagt atttatgcat tgcttatctt ttagtttacc taatattgac aaaattcttt
     4321 tttcacataa atccataaat tcatcaaatt tattatcatc aaataaatca aaataagcat
     4381 cattaaaatt tttgaaatta ggacattcca tagtattatt ataaatgtat tcccaacaaa
     4441 tccaccatcc attagtacgt gaaccatcat catttattaa ttcttttatt tcatcatcac
     4501 ttagaatctt tccgctaggt ttttctttat ataacgtaca aaaacctaca aatatatgat
     4561 gatctatttc aattcttagt aaaagatcaa catcttcttt atctagactt tttataaaat
     4621 aattaattgc tggatatgta gagcttttct tattataata attattcact aaagcataat
     4681 tattttcttt gtatgagtaa tatggtaatt tatatttatc ttcaaatttc ttatccatac
     4741 gtttttcaat ttcttctaaa acctttttta tcatttgtgt tttacataac tttaatcctc
     4801 tttcaatttc tattgctact cttatatttt ctttagatga taaaatttct actatctcat
     4861 cctctttttc tagtgatgat acatttgtca tatttcttat tacctccata aattgcagta
     4921 taatttctct tattggagct atttttatag tttcatgatg tgagacacat ttatttagcc
     4981 aatttaatat atctatttca aaagatattt gagtaacttc tttattaaat atactattat
     5041 ttatttaaaa cgagttgcag ttgtgttttt aataattata actggattat tcacaataac
     5101 tatgagtgtt gataccctta gaaatcaatt tatcaaaatt gtaatagaaa tttatgaaga
     5161 tttaacagaa tttatttttt ctaaagatat tcaaactgat aagaatcttg attttatatt
     5221 aaaagaaccc aaatatatac caaatggttt caaagaggtt gaaaaagaaa acacattaca
     5281 aggtatcttt attcgctatg aaaacaagaa tggctctgaa ataagatatc gttccaatca
     5341 aattgattca aatacagtaa tattagatac agagaatgca aaagtcacag atattatggt
     5401 aaatgattat gaagctaaat atattgttaa agacaatagt attacaattt tttggaatga
     5461 tgaagaaaat gtatatacaa tatattttga ttatgataat aaaaatgatt taaataagta
     5521 taaagataat cttatagata ttgctgaaaa tataaaataa tttttttcaa aaagtgtcac
     5581 aaatcacctt ttttttcgtt atatgagtat ataagaaaaa aggaggtgat atttgtgtat
     5641 ttaaaaaaag tgattgtagc aggattatgt acattaactt gcctattacc tttaaatgtt
     5701 caagcttttg aagatacttc attaaatact aatgattata atcaaacaaa tgataatgat
     5761 ttatttatga tttatattaa aggtgctggt agtaatttga gtattcgttc tggaaaggct
     5821 catatatctt gttatgttga tggtaaaatt gggagtttaa catcaattac agcaagacta
     5881 cagcaaaaaa aatctggtaa gtgggtaact atatcaacat ggtcaagctc aggaaaaact
     5941 tcaagtactt tatctagaga atatagagta tcaaaggggt attcttatag aatattaaca
     6001 acaataaaag ctggaaatga aagtaggaat ataacaagta atgtaacaaa ttattaattt
     6061 aattaaaatt taggaggaat attattatga aatattttaa aaaagtaatt gcattaggac
     6121 tatcaattgc attgcttatt ccagtaattt caatcaatgc attagaaaaa aaagacacaa
     6181 atgtaaataa ccaaataata aatgttatag aatcaaatgt atcttatacc aaagagggta
     6241 ttgaagtttc ttctgacttg aaaaatgatt tgaaagaaat taacttagaa gatattaaag
     6301 aacaaatgat agagcaagga attgattcaa aattcataaa taattttaat agtgacgtaa
     6361 tatatagttt agttaaagaa aatatagatg atataaatga aggagtaaaa aataaaactc
     6421 ttaaaattac acctgaggaa gatattataa acaatacaga caactcattt tatgtacaag
     6481 gaggttctac atatactcaa aaattttcat ggggagtaag acaaaataaa agtactgcag
     6541 cagcaaatag atgggtttat gatttgaata agtcttcaca aggatttgta gcaacaggag
     6601 cagtaagtgt tcttttaggt tctatgggag caccaatagg atttgtatct ggtttatcag
     6661 ctgcatatat gggaggtctt gcaaataaag tatcttatgt aaattcacga aataatagag
     6721 gaataactgt ggatataaac tggtggttaa cttatgcagt aaaaaatcag taaaaattag
     6781 gtgaaattaa tgagtgaaaa acaaaactta aattataaaa aaaaaatctt aaaacaataa
     6841 tatttatatc ttgtttaata attttatcta tatttatagt ttttaataat ataaaatctg
     6901 actgttggta tgttaattat atgaattata gtgatatgca tgaaatatct agtgggaaaa
     6961 gtcaaaaaat agcattgata gattctggta tttcaaaatt tcaaaaggtt tataaaaata
     7021 taaatttaac taatagcaat aatgattatg atacaaatgg tcatggaacg atgatgtatt
     7081 caatcataaa aggttataat aaaaataatc aaacgattga aggaatatct ccagattcta
     7141 gaataatatt aattaaagta atgaatgatg atgaaagtat taatcctaat gtaatagaag
     7201 atgctataga ctatgcatta aatgaaaaag tagatattat tagtttaagt ttaggatcca
     7261 caaaaacaga tattaatata aaacaaaaaa tagataaagc taatcttaaa aatataacag
     7321 ttgtaagttc agcaggtgat tataaacaag gatttttgct atttccagca tctttaaata
     7381 atgttatttc tgttggtagc atagatgata gaagagatat ttcaacattg acaaatgcac
     7441 ctcaaaagac attaataaat gcacctggag aaaaaataaa ggtgatagat aataacaata
     7501 atgttttcta tggaaaggga acatcagaag caacagctat tattacaggt tacatagcgt
     7561 tgatgagaga cacaaatatt tctagtactc aaattttaac taatgaaaaa attatgaatt
     7621 tattatccca gataaacaaa aacgaaaaaa catatttagg agcattaaaa gaaatcagaa
     7681 aaaaagtatc tgtaagtaca ggtgcttttt tctcttttga tttatcatat taaaaatagt
     7741 gagaaatatt ttacttgaat aaattctatt tgtttataat tatagtatgc atttaaaatg
     7801 agggggaaaa tagtatgttc tataacaagc aaattaaaat attaacttta ttaatttcta
     7861 tcttaatagt tttaatatca gcactttctt gtactaatat tattaaaaat tttaatttat
     7921 taaaattttg tttaatatta tttggagtgg aacaaatttt actagcttat aattcttata
     7981 agcttaataa aaatctaatg gagacacttt tattatgtat tattggaata atccttatag
     8041 gtatttctat tgtaattatg tgaatataaa taacacgttt tgaatggaaa attatattaa
     8101 tttagttcac taatattaca tctttaatgc aagtagagta aagtattttg aataacatag
     8161 gtcaatataa attgtaggag tgattttcaa taatatgtat attcaaagta acttttctat
     8221 ttttgttact tgcaggtttt tatcttgaac aaacgtataa attttaataa aatataaaaa
     8281 taaactttat atttcaatta cagatgatga tattttgatt gataataaat atatttttag
     8341 aggcaagaat attaaaaact atattaaatt taaagatata acaggtgcaa ataaacagga
     8401 tagtcttata tatctaaaag ctttaagcat tgagagagaa gaatatctaa taaatagact
     8461 aaaatcattt ttctgatttc atatatttta aagttttaat taaaagggtt gcagatattg
     8521 agttacaagg tcttttttgt aatataaaaa acaccacttt gtgctcatta caaatttcaa
     8581 tactttctgt atctaaaagt attgaaaata ctcaatttat tttggaattt tatactacac
     8641 atgcataaaa caattttaaa acaattgcaa tattaattca ttttttttct accatattat
     8701 gatttgagtt gcttttttta attatagaat ttcctaattt taattcattc tcaagatttg
     8761 cttttgaaag tttaagagat tctaatttat catcttctgt aaacttattc tttaaaattt
     8821 cttggagaga taagagttca tttttcttaa atgaatattt ttctttaaaa ctttctaatt
     8881 tatttgaact aattttatta taatccatga aatatttaat tagtgtttta ggactttttg
     8941 atgtagaaaa ttcaaattta tagttattac ctagagaaat gaattctcta actttattgt
     9001 caaatgtaaa ttctttttca taaattatgt tcaagccata tatttcacct aaaattccta
     9061 cttttccatt atttattata tctgacagag cttctatagc atctttaata tcaatataag
     9121 tttcattatc taatgttatc ttaaattcat taaaaaaaat tagaaaaata cgttttaata
     9181 tcttcttcta atttagaaat tgatatcttt aaagcatcta ttgaattttc ggttgatttt
     9241 atgtcattta aagtaagtaa attttgattt aaaaaggctt ttttatttat ttcaattatc
     9301 tgaatttctc tatttatctc acataatttt aatatgctat catcagaaca ggcacatgcc
     9361 tttgtttcag cataactcat aagctgatta cctatgtctt cagcagtcct agaagatgaa
     9421 gaattattaa gaatttgagc tatatatttt gcctttattt ctattgtctg tcacgaatat
     9481 gcatcaaaag aaccttcaac tacataccta aaaatataga cctctttatt caaatttcct
     9541 tgccttaata ttctaccttc tctttgtgaa attgatgaag gtttccaagg aacatctaca
     9601 tgatgaagtg attttagagg tagaaccaat taatattcta ataataccat tattgaaatc
     9661 atcaataagt ttctttcttt tttctggagt attagcgtca tgtatgtatg ctatatcttt
     9721 ttctaaaaca ttatttttta gaagttcatt ttttatatca ttataaacat tatgtgaatt
     9781 tccattagga gttcctagat cacagaatac tagatgtgta cttattttat cctcatgata
     9841 taaagaagct atattttctc caacttttat tatctttggt aatataccat caattccaac
     9901 taatgcaggg cttacagcca tcaaacgacc atctgtagtt acatttaaca tgttatctat
     9961 tattggatca tatgggcctt catgaatttt ttctgcacgt tttacaagag aatctatata
    10021 ttctttcatt tggtttgtag gttttataat ttctattata ggttttccac cttttatctg
    10081 aggaatatct aaatcattta tgtcacagat gttaactaca tcagcaattt gtctgaatat
    10141 attaactaat tctggaacat tacagaattt agcaaatctt tgctgaaatc taaacccttg
    10201 accagtagga tctacttcca ttgaggatat aattgaacca aatttacttg cccaagcatt
    10261 gaaagtatct actttgtaat actttaatgt atctttattt aaataagtga gtaaagtata
    10321 catttcacat atactattag aaataggtgt agctgtggca aatgtaacac ctttatttcc
    10381 atgaatagat ttaaaatatc ttagtttgtt gtataaatcc tgtgtttttt tagtttttaa
    10441 agttgatata ccagggactt gcatagttgt attaaaatat aaatttttga agttgtgagc
    10501 ttcatctatg aataagtttg ttattccaag cttttcaaat gaaagaagag catcgtcttt
    10561 atgcatatca tttagctctt tgattctatt tagataattt tctttcattg tttcaagtcg
    10621 ttttgtagaa agatcagggc attctttttt gtaatattta agagtatcat ttaattcttt
    10681 taagtataag tctaataatt ttaattctgt atcagcttct aaagggatta atcctaatac
    10741 cgaatgtgga attattatac tatcccagtt gcatgtcaca attttagcta taatttttct
    10801 tctattggat ttagagaaat cctttgaagt tgtagctaat atattcgctt gaggatataa
    10861 attaaaatac tctttagcaa attgtccaga ttcacaaagt gaatttggta taacaaataa
    10921 attcttatta cctttctttg aatctatttt agaaattcta tttaattctt gtgcagctgt
    10981 tatcattgta taagtttttc cagaaccaac actatgacat agaagagtat tacgatcaga
    11041 agtaattatt ctactagccg cttttaattg atgttctcgt aattttatat taggatttat
    11101 tctaacatca tttaatatat ttttatattc cttttcctta taatctataa acatttcatt
    11161 gtatctatca agtaattctt tatgaatata actattactt gttacatagt tatgaaaagc
    11221 tataagccat ttatttaata tgttcatagc aagttgagtt tcttccaaat ttttaacaga
    11281 aacttcttta ccattttgat attctttatc agtgacagtt atagttttca tatttaattc
    11341 tgcttcaaca atatacaaag attttctcct ttttgtaccc catgtaaatt catttttact
    11401 tataggtata atgtcagaac attttactga ataaccaagt tgtgttgaat acaataattg
    11461 aattgaattt tctttgagtt ctaatgtttt ttcaataaaa tcagatttta tacttgaaga
    11521 tatccaagta gaatcaatgc taaagaacac atctgtttca tatttaggtt gattagcttc
    11581 taaagcttta acattaacca ggaaagtatt atcttttatt gatgcttctt tggctatttt
    11641 taatttttct ttaacatatc cactcaaata attatctcta tctactaaat atcctgtcaa
    11701 acggtcaata taagcaagtt gtttagataa taattcattt ttgatattat gatatgattg
    11761 attcatgatg ctacagatat aatcaatatt taaatatcct ttgtactatc taattttgag
    11821 taaaataatg taacttcttt ttcttcatta ttttccttag atttaaattt ttttaaaaca
    11881 gtgtttgttc cagctggata ttttacttca aatcctagat tgaataaaaa tctccttgtt
    11941 ttaagaatat tttgtttgtt aatttcgtta aagtttttaa atgtttcacc taattttttt
    12001 gatagagtaa agataaagtt tttgatttca taaaaacctc cttttatttt ttatgctaat
    12061 agttacagct tatttttatt tgctaaagta tagctttaat ttaatttgta tataatatgg
    12121 ggcatttaga agtctttttt gaaaattttt ataaaaaatc aaaaaaactg ttattaaaat
    12181 gatttataat cacttaataa cagttttaat tattaacatt attttttata gtacaaataa
    12241 gaatgtataa tctaaaaacg tttaagaatt agtatttttt agttcatatt tccaggtttc
    12301 accacttttt attttttctc tctcaaaata atttggatat ataacaatag gaaaaggagg
    12361 cttataaata ggagtaatat tatttaaagt acaataacgg atgttaaaat cttcatatct
    12421 tagagtgtta tcttttgtaa tccagaaaaa atcttctaca ctcatagcaa gattacttct
    12481 acaaatatcc caaacgtcat aatatcctag tttaaattct tttagtataa aaggaacctc
    12541 tggtctgcat tcttgaataa ctctttcctc aatccaaatt cttataaatt catcccattt
    12601 atcactatct aaattcttga aaatatttgt gattggaaat gggttcattc ccttgtaatt
    12661 tttattttgc ttaaatgaga atttatcatt tattatatta tatattaaga ttcctgccgt
    12721 atcgttttta tattttaaaa aatataagtc gttatttctg attactccat tatttttgat
    12781 gattagttta tcatcaaaat ttagcttatc ataaaataaa ttattcatat aaaactcccc
    12841 tctcttttaa atattcaatt cttactttta acaagtgttt aatacatttt aatctatgtt
    12901 gtctcaaaag atctttatat ctatcaaatt gtccctagaa aatagtattt ttataccctt
    12961 atttgttata cataatttta tttagaagat aaactttata taaaacaatt aaaaatattt
    13021 ctattaagat aaataaaaaa gagctatctc agaacaaatt taatttgctc atgtgacact
    13081 atattataaa ataaaaaaat tttcacacta ttttttacac tatataattt ctatttttat
    13141 aattaaaccc aatttataag atatagaacc atatttatat ttttatctct tgatttgttt
    13201 taattacata tatttatttt ttatcttatt taataattac tattacttga atatttactt
    13261 ctttgagtaa atttcatagc taacaaagct aaaactatta ttatagaggt caaaatacaa
    13321 atataattca tattaaaaaa gcttccatct ctttcttcta tataagtcac aaaactaata
    13381 aaccacctta cagggcttat aatactaatt ttttgcaaca tttctggcat tttttcaact
    13441 gagatgaatg aacctccaat aagtgataat acaagtgata tacatgtaga aaataaattt
    13501 gcatgcatct ctctattgaa taaagaagat acaaatgttc caaaagaagt tgccaaagct
    13561 actacccata ttcccattaa aattaaatct atataatcca taccaatatc aatattaaat
    13621 aacttaatta tagatattga aactatgtac tgaaaatacg ttattgtaaa gttgtataac
    13681 atattcccta gcacatatcc tcttacattt tgtggagaat acagcactct tgtaaatgta
    13741 cctgagtttt tatctttaat aatcattgat gctgttacag tagatgttat cattaaaaat
    13801 aatacgataa atgcaattat tctttgtaaa actcctaaag tattttcttg taatttactt
    13861 atatctacag gaactgggtc ttttttatat gtttctatta aatactttaa tgtatcattc
    13921 gtatttttat tttttataga atataactta aaaccattct tattaaaata tatgacagct
    13981 gaatacttgc ctgttataat atcagtttta attgttttaa aatctgcaat ctttgtatta
    14041 atcccttgag tattttctaa agccattatt gtattttcat aggttctttt ttcagattcc
    14101 tttagaactc gttcgttttt gtactcatct aaaataccaa tattaaaaaa tggtttactt
    14161 atgtaatttg ccaatactcc caaaattact ataattatag gaaataaaag tgtaactaaa
    14221 aatactattc tcttatttat atttcttttt aagttaatat taaaaacatt tattacattc
    14281 actatataaa gacccccttt ttaaaaaact tgattgtaag tattgtcata aatatgctta
    14341 caactacaaa tatgcatgat atattaaaaa ggagttgtaa attattatca tagatacaag
    14401 caactatgcc tctattaatc catgttaatg gtgatatatt aatgattaat gaaataactg
    14461 gattcaatga actaactgga aagaacactc ctcctaagaa tccaaaaata actatcggta
    14521 tattaataat attacgtaac atatctaaat ttttacaaac aagacccaaa aacaacccca
    14581 tagatactac cacaatagat tcacacatta aaaagaaaag aacagctaaa aattttccac
    14641 caaaactcat acgaaaaata cattttaaca ccactaaagt taccaaattg catatcgaaa
    14701 gtactgctgt acaagaaaaa agttttgaaa taactaaatc tactattgaa ataggagaaa
    14761 tcatataacg atatgatgtg ttagaaattt tttcatcttg tcccgcatat gaaacactaa
    14821 taattcccat aagaacacaa aatggtattg taacaatagt ataataatta tatgatgtaa
    14881 attcctcacc ataggacttt gatgtcaaat atcctaataa cattatgatt attactggaa
    14941 aaacaatatt gtaaaacaga ataaaaaaat ctttacttct tcgttttatt cctaatttaa
    15001 acaatggtaa aaaacatctc atctatttat tcctccaagt ctctcaaatc tgtacctgta
    15061 agatataaaa aaatatcttc taatgtctta caaccaaatt ctaaatattt atttttatat
    15121 aaactcttgt ctaaatcttc tataattatt cctttatcta ttaaagcaat tctattacat
    15181 aaggtttcaa tttcttccat ataatggcta gtatatataa ctgtcgtacc attagtatta
    15241 atgcatttga ttgcctccaa aattttattt cttgattgag ggtcgatacc tactgttggc
    15301 tcatccatga tgagaatctt tggactatgt gcaattgcac aagctatgtt tagtctacgt
    15361 ttcattccac cagaaaattt tgatggaaac tccttatggc tttcccaaag accaacaaat
    15421 tccaaagcat ctttagttct ctctttaagt tcattccctt taaatccata taatgagcaa
    15481 aaaaatttaa cattatcata tgcacttaaa tcttcatata ttgctaattc ttgtggtaca
    15541 actccaagtg aacttttaaa aatattttgt tgattcttaa tatcttttcc tttatagtat
    15601 atgcttccac tatctggaga cacaatcgta gtaagcatat tgatagatgt acttttccct
    15661 gctccatttg ctccaatgaa acctaaaata tcgccttcca ataacttaaa actttctatt
    15721 gacacaactt ccttattgtt atacttctta gataaatttt taatctctaa caatatatta
    15781 ctcatttata accccccaat atttaaccaa cattatatat ccaatattgt atatataatt
    15841 tttttaaaac acgagtattt tacccgtgtt attgtaataa aatttattac ttattttgta
    15901 attttagtat tatcttatct atgttttcta tagctttatc tgggttatta attatttcat
    15961 ctctaagtct ctgaacttct aataactcat ttgtcgtatc agatgtaaat aaaaaatctt
    16021 cttctatatt tgaaaaatct atagatttaa ttatattctg tgtctcgcaa ccaacggata
    16081 tatacttatc aatatcattt aatatttctt catgccataa aatttgataa ttcagattat
    16141 ctatagtcat atcaaatgca attttttctg ttgcaagact ctttttatca atttttttaa
    16201 tctctttcca attttcccaa tacttcttct gctctataag atattttata tgtttttcaa
    16261 gatttttttg caaagtatcc ttaggtaata catccaaaat attatgtaat aggaatttat
    16321 ttgaaccggt tggaactatt ggcatctgaa gctctttttg tatttcttcc cttaattcta
    16381 atttaccact ttgagtaatc tcatagattt tacgaatcct agctcctgtt ttttcttctt
    16441 ttaaaactct aacgcctcca tctttttcta attttgccaa tgcataatat attgacccag
    16501 attgtatttt agtccatcta tctgcaccag atacttgtaa aaacttttga atctcatacc
    16561 catgtgttgg cttaatattt agataatata aaattaaagt gcgtatcata aaaatccacc
    16621 acctatactc tatcttggat attatattat caccaatctt ggatatagtc aatagataac
    16681 ataggatata ttacaactta ttatttttat tatttaatac aaggagttcc atgagacata
    16741 catatccatt catattctaa attatccaat tctcttatag atttcatcat ctttttagaa
    16801 tcccaattcc agggattagg gtaagaaact atatttccat tcttgttttt gactaaatca
    16861 cctgcaaata atacatcttc aaaaatcatg cagacatgcc ctggagtatg accaggagta
    16921 tggataattt ttatattccc aatctgcata ttctctccat aaggttcaac atccttaatg
    16981 agccttcttg aaattgtctt tccaataaac ttcttaaacc caggtctatc tttctctcct
    17041 ataataaaag gtatatcttc tgtatgagcc catacctttg ctcctgtttt ctcttttaat
    17101 aatttaatat tagacatatg gtcaacatca tgatgagtta ataaaatatg cttaatatct
    17161 gtaagtttga taccaagtgt ttttaattct ttaattatat ttttccccat aaaaggaaga
    17221 cctgtgtcta taagagtaat ctctttatca aacacaataa aacaatttga gccacgagta
    17281 ctatctaacc ttaatacatt ttcttttatt ttcataaata aattccccct atttggtatt
    17341 ttgcctatat aattaattaa tgaagttata aatagctata atacttgata ttaattttat
    17401 tttcattaaa gtatactaat agtatacaaa tatattatat ttacacaagt ataccaccag
    17461 tatactttat tatcaataga aaggaataat ttatatgtca gaattatcaa aaagagataa
    17521 agaaaaaata caaagagaaa atgaaattat tgataaagca gaaaaattat tttctcttaa
    17581 tggatttgat aatacaacta tgaatgagtt ggcaaaggaa gtcgaatata ctaaacgcac
    17641 catatacaag tacttttcat gtaaagaaga tttattcttt gctgttgtct taaggggata
    17701 taaaagactt tgggataatg ttaaaataga aagtgcaaaa ggtaaaacag ggtttgaaaa
    17761 aataaaactt tcatactttg cttttcataa attttactct gtagaacctt ctctattatg
    17821 ccttatggga atgattggta ttgtaaactc taaaaaatcg gatactgaga tgctgtttaa
    17881 agaaaagttt ttttctttta ataaatttat gtttgatgaa atacaagaaa tgtttgaaat
    17941 tggaaaaaat gatggaagta ttaggtatga tattgaaata tctactctta tgtattcatc
    18001 aatatttaca cttacaggat tttttaattt attgtctgta acaagaaaat catacctgaa
    18061 caattttaat atagatgagg aaaaatttat tgaaacaacc cttgccattt taatagactc
    18121 gattaaagca tagacctaag tatataaatt aaaatttata tacttaaatg gtaatcttta
    18181 ctatatataa tataaaaaat attaagcata aaactaataa tttaaattaa tgaaaggact
    18241 aaaagtaaat ggctaaaata cttctcttag aagatgatga tacaattacg tttgggataa
    18301 agacttccct ggaaaataaa aatcacattg tatgttgtta taatacgatt gctgaagcta
    18361 aaaaaaattt tgataaaact actgatttaa ttctgcttga cttaaatcta cctgatggta
    18421 ttggatacga attttgccaa tacgttaaaa gtatagaaga tactccagtg atatttttaa
    18481 cagtccgtga tgatgaaaaa gacattatac gtggattaga tatgggtgca gatgactatg
    18541 tggttaaacc atttttgctt tcagtacttc aatctagaat taatgcagtt cttagaagag
    18601 ctcattctaa tactaataat actattactt gtggaaatat aagtttgaat aaagttgaag
    18661 tcaaggcata tttagatagt gttgaagtac ctctgacagc aggagaatat aagcttcttc
    18721 taagtcttat ggaaaataaa aatagagcta tgactcgtac aaaattacta gaaatgctct
    18781 gggatgtaga tggtaatttc gtaaatgaca acactcttac tgttacaata aagcgtctta
    18841 gagaaaaatt aaaaaatcca ccttgtataa aaacattaag aggtatagga tatagaatgg
    18901 aggacagcta gtgaaaactc caagaaatat caacagtaca gttgttttta taatattctg
    18961 cttaattgta acttttttat tgagtgtaat tctaacaact atcacttctt ttaatgaata
    19021 caatgtgctg tctaaggttc ttgatgcatc atatgagtac aatcctaaat ctacagaaag
    19081 tatactaaaa tcactgaaaa attctagtga tgaggatttt aaaacaggaa atgaaatttt
    19141 atcaaaacat ggatattcta aaggtacatt ttggagtaaa aacttttttt attttctagt
    19201 ggtatcgttt ggtataattt caacaatggc tatagttgta tatataatca agtatagaac
    19261 aaataagcaa aaattgagta gaataaacat gcttactcga tacttagaag aagtaaattt
    19321 aggaaaagag tctgtacttt taagatatga agatgaattt tcttatcttg aagatgagat
    19381 atataaaaca gttggggaac ttcgttgttc aaaagaattt gctctaaaag aaagacaaac
    19441 tctttcagat aaccttgcag atattgctca tcagcttaag actccaatta cttctatgtc
    19501 acttatggca caattacttt cagaaaattg tccagatgaa gaaatagatt atattaacag
    19561 gcttacaaag caaatatcaa gacttgagag actagtttca tctttactta ccttatcaaa
    19621 attggatgcg agtactttaa tatttgaaag caaacttata gatgtacatt ctcttcttac
    19681 ttatgcaatt gagcctatag aaggttcttt aagagaaaaa catcagacat ttactatcaa
    19741 aggttctcct tcaatagaat ttgaaattga tattaattgg actttagaag ctcttttaaa
    19801 tattcttaaa aattgtagtg agcatataga agctggtgga catatcattg cttattatag
    19861 tcaaaaccct ctatatgttg agattatcat agaagataat ggtaaggggt ttgctcagga
    19921 agagctccca tatattttta atagatttta tcgtggtaaa aacgctagta aggacagtat
    19981 tggtataggg cttgctctat caaagtctat tatagaacgt caaaatggta ctctacatgc
    20041 agaaaattgc cataatggtg gtgcaagatt cataattaag ttttacacat aaaatactgg
    20101 attataggta aaatacccat aatccagtca tatctatatc ctatgttatt tattatttta
    20161 gtaatccgct atttatatct aagattttat taaataaaca atttaactat ctctatactc
    20221 gtaagtattc ttgaattgaa accataaata agttcagata acttttattt atattgtttc
    20281 actttttata gattctacta tattttcatt cttcaactga ttgtatcctg tcatatatga
    20341 tgctattaca ataaaaagta tcaatatagt aaaacctatt attgtagata taggtgcaaa
    20401 tggtaaatac tctataaaat atatttcatt tatacgtaga aaagtatata ctattcctat
    20461 ttgcacagga atactaagta gtaatggtgt aattcctaag aataaacctt ccaaaacaag
    20521 catctttttt atgccatctg gagataatcc aacagaacgc aacatagcaa actcttgtcg
    20581 tctacttcta atatttcctg acactgtagc aagtacattt gataatccaa tcatagccaa
    20641 taaacctgta ataaaaccta ctatcaaatt catagtcttt cttccactac cattttgttt
    20701 ttcattttct acaacatcct caatactata gtctcctgaa ccataatctt tattacaaat
    20761 agactctaat tttcctctta ctctaggaat atcctcacga ttatctactt taaacaatcc
    20821 tatcagtcta tttgaacgtg acctacctac ttcagcataa tttttagata tttcttgcac
    20881 catagatatt ggtactattt gcattaatgt atatcttgaa ctagtatcta ttattttagg
    20941 aagtgtgctt gtaatagctc ccactttcat gttgtaagta taatcccctt tatcttcatc
    21001 atatattttt tcatttaatt taatgttatc tcctattgaa agatttaaat aatctatata
    21061 tactttattt ctacgtgtgc tacgattaac atcttcctcc ttattatata caattgaagt
    21121 tggatttttt gtatcataaa ataattttgg gtctacagag atttgtctgc aatattctgc
    21181 gaaactctca tcatcaagcc caattatatt tgaaattata cgaaacttgc catcacgctc
    21241 tataggagag tactttttag atgaaactat acttttaaat cctccatttt tcgaaagttc
    21301 ttttgaagca tctttttcag tcagccaagt ggtagcagac atcttttgat aatatagtga
    21361 gttctttaca ccttctgtat tatctaattt ttcaatgaaa tcagactcta caggttcact
    21421 attttccaaa taaacttcta tatctctttg gtctttataa ttatcacttc cataaacagc
    21481 ttctgctcct tcttgatttg caattatatg caaaaaacct gtcagtaata aaaaagataa
    21541 tgttaaagat attgttgcag tacgataaga ttttttacga gcacgtagtg catttgatgc
    21601 aagttctcct tctattccaa atatctttcc taatgaatat ttatgtgact ttttaatttt
    21661 aacactatct ccttgtcgta ttgcttctat aggagatatt tttgatactt ttcgtgctgg
    21721 tatgagtgta gaaatccaga ctgttactat tgtaagtaaa actgctggca gaaaagcagg
    21781 aataccataa gtgaatacta cttctggagc atcagtatga taattaactg aattaatata
    21841 gactataagc ttattacaca ataaccaccc taaaaataaa cctattggta atggtataat
    21901 agtcaataaa aaaccttcaa acactacaga ccttttaatc tgttttggtg atgctccaac
    21961 actagataat attcctaatt gtgtaagtct agcatttgca gatagtgaaa atgcgttgta
    22021 aattaccatt acaaacacgg ctactgtaaa aattgctatt gtaagataca taataggagt
    22081 tgaaaatttt gatatattgc tcatagatgc tttctgttct ggagaaaaga ctaacatttt
    22141 taaaagataa ttactattgt atttaagatt gtattttcca tattcattag tcttccatcc
    22201 caatgattta gcaagttttg gaagttcctt atatgtgtct cttatatttt taaatcgcaa
    22261 gtacacagtt atactgtcat taggttttat acttgtttta tcaagatatc caagagctgt
    22321 atatgcaggt acaactgatg aagtagcaac atctaccttt cctactattg tcaatgtagt
    22381 ctttgaaatt tttttaaaac tttccccttt cgattttggg gctacaggtt caagaggctt
    22441 tccatttagg attctatctc ctattgggag agtaatttta tcaccaattt ttacacttgg
    22501 attattctca aaatattgtt ttgatagtgc aatttcatta gtattacttg gtcgacgccc
    22561 ctctattata gaatcttttt ctggcatatg attccaatac tctgatgtag catttctata
    22621 aactatataa tctcttttgt cattattaat tttgcctaca taccattgtc cttttaccat
    22681 tacatcatca actgaagaaa aattttcaat taacgataag tcttttccat aagtatcgtc
    22741 aaacaattca ccatgccagt taccctcttt ctcaattgaa agtcttataa aatctgtcca
    22801 catggtatac aacaatccac acaaggaaga catcatggtt gtggtaagta atatggctaa
    22861 cattatagct atactactac gtttattgcg tcgtacaaag tctaaggtat attcttgaag
    22921 aatattcatt tcttcaccac cttttcagag acaatcactc catcttccat agttacaata
    22981 cgatttgcct ctagtgctat tttctcatca tgagtaataa gaagtattgt ctgattaaat
    23041 cgtttatttg ataagtttaa caaatctaca atttcttctg aattttttct atcgagatta
    23101 ccagttggct catctgctaa taaaatagtt ggtctataga ttaaacttct agctatagat
    23161 accctttgtt gttgcccacc tgaaagttga cttggaagat gattcaatct atcagataat
    23221 cctaatatag atacaatctc agagaatcta tcttcatcca cctttctttt atcaagtaac
    23281 atgggtaata atatattttt tctaacatca agagtaggta taagattata aaattgataa
    23341 ataagaccta cttttctacg tctaaaaact gcaagagctt cttcatttaa gcttgatatt
    23401 tctgtatctt caacataaat ctttccagat gtaggtctat ctactccccc aagcatatgt
    23461 agcaatgtag atttgccaga accagatgct cctacaatgg atacaaaatc acctttttga
    23521 actgataaat ttacattatt aagagctgtc actctggttt gatttgaacc atatattttt
    23581 gtgagatttt cacattttaa tatttccata tacactcatc tcctttatgg ttaaatttta
    23641 gcaacctaaa atgacattat agtgacatga atacaaatat cgattactat taaaaatatt
    23701 gtttagtttt tgaacattat ttaaaattat aatacaaaac aaaaactatg caatatattt
    23761 tatttatact tttaatatat tgcatagcct ttaaaagata ttaactttaa tatctataag
    23821 tattcaattt aataatcaat agcaagtatt ttatatacta gaggagtttt gctctatgta
    23881 aactcaattt agtaaaatta tttaataaat atttacagac tataggtaac caatatttac
    23941 aaataaaatt aagtttatta cattacacta taatatttct taattatatt aattgcaatt
    24001 tcctttacct ttggtcctgc aatttcttta ttggaatcat ctgatttaat agcaaaataa
    24061 taagtgtcat ctttactctc tagcatacca acaaaccatg catctacacg tttatttttt
    24121 ccattaaatc ctgttccagt ttttccatat acatttatat ttttatcact tacatctatt
    24181 ttcataatat cttttaaaat attaatatgt tcttttttaa agtttgtatc tccctcaaat
    24241 attttcacca ttgtttgcac ttgttctttt ggagatattt gtaatgaaga ttctaaccaa
    24301 aatccattta aatgcccctt tccatttttt aaatctcctt cccattcact aatatcacag
    24361 ttcccatact ttaaatcatc taaaatactt tggacagatt tagcatcaac cttatttatc
    24421 aattttttgt agtaccaaac acaagactcc ttaaatgcct cttctaaact aagattttta
    24481 ttccaatttt tatttggata atttgtacca tcatacccaa taactgattt ttttgacgtt
    24541 attacccctt tttctaaacc aattaatgtt gaaattattt taaaagtaga acaaggtgaa
    24601 gctcttgttt caattaaatc tttgttatat atattatatt cttgatttct gctattataa
    24661 aaaatagctc ctccactaat accttcaaaa caatcactat agttaactat attaactctt
    24721 tcttttttat tgttatcatt attaatttca gatttattta aatcagattt tatgtaattc
    24781 ttatcagttt tttgatttat atcgttatgt tttttacctg cataatacat taccataact
    24841 ataccaacca acaagatagt tgtccaaata aaattctttt ttcttttcat aatttatatc
    24901 atctcccata tttaaattta taacctactt ttaaaatagt agacattatt tgtaatatac
    24961 ttcaatctag gtgctactta taaaaaataa gactaaagga ataactatat atcttatatt
    25021 ttcttcacct agcacaacta tgtcgattaa acctattacg aaaataatta ggtaactatt
    25081 aatagcattt tgtcctcctt ttaatttgtg atatagtatt tgattctact tttaaaactt
    25141 actattgtaa tatttaaata ttacaatagt aagttttaaa agtcaagtta gtttgagaaa
    25201 gttattttaa ttgtataatt gttattacat ttataaaata tggcacattt aaaattattt
    25261 tggtattggt agtattaata taataaatat tataaaagct ataaattata aaatttaata
    25321 taatttatag cttttactct ttcaaatatt attttttatt gacttattaa tcaattttat
    25381 taacaggtta catttatata agtaaacact taaataataa atatataatc tacatatcta
    25441 taagctgagt caattcacca gtatatgtca acttaagttt gtgcatagct cgtgtacaag
    25501 caatatacaa caaactacgg tcatagtcac tatagtatgt tttactgttt actgaaggta
    25561 taataacttc atcaaactct aaaccttttg acattttaat agaagttatg gacactcctt
    25621 ttgtaaaact agaactttca gaagatatta agttaacact atactcttgt tttaaagcat
    25681 tatatataac ctctgcatca ctatctgtct ttaatataat acccaaagtt gcattatcac
    25741 ttttcttaaa ctcttcaatt tctattttta tcttagatac ttcatcttgt ttattagtac
    25801 atttaactaa agctggttct tcgccatgtc gttcaatagg ctctaaagaa gatacatctt
    25861 gaaccttctt tgcaaaatta ataatctcaa aagtagaacg ataactcttg ttcaatgtaa
    25921 ctagttctcc ttcattatat agttgtctca tatcatctag tgtatgagta tgattaggat
    25981 taaccaattg tccaaagcct ccaagaatag tctttttaca cttaaaaagt aagtttatta
    26041 cagcatattg aataggagta tagtcctgca tttcatcaat aacaacatgt cttataatct
    26101 tatcttcttg tatcccttca tatgcagcat gaatatatat aaatgggtat acatctgacc
    26161 attcaagagt tttctttgtt gccataacaa acatatttga tagattcatt tgtttaaaga
    26221 aatccttata tagtgtaaga gtatttttta ttgtcaacat tccattcaaa cttttaagta
    26281 tactttttaa ctttggtaaa tcctcttcca taatattatc tgactcaaac ttataatgaa
    26341 tgtcttcagc taccttctct aatcttttct taactggata tctattatat gcctcaaatc
    26401 tactctgtat ccagtcactt ttagctgtaa aacttccaaa attgtagtct tttgggataa
    26461 aaatcttatt tggcatttgt tttatataat aatcaataag ttttacaaaa tcaaatgtag
    26521 atttaaagcg cactctttca gcccatttag catcatttat ttctaatggg tctttctcac
    26581 tttcaaaatt aataactcta tctaattgta cctctgctat attttcaaaa cttaattcac
    26641 ataatggctc ttctccaagc tcgggaagga cattggaaat atagtctcca aagactttat
    26701 tcggtgatag gataattaca ttgtttgctg atattttatc tttaaatcga taaagaagaa
    26761 aagcaattct gtgtaatgct atagatgttt ttccagaacc agctacccct tgaataatta
    26821 gtgtatgagc tttatcattt ctaataattt gattttgttc cttttgtatt gttgaaataa
    26881 ttgacttcat cttttcatct gaagtgtgac taagctcctt ttgaagaata tcatcttgta
    26941 tattaatgga actttctaat gcgtattcca attttccatt tttaatttta aattgtcgtt
    27001 ttcttgttat ttctccatcc acccatccta ttggagcatc atatcccgct tttcctattt
    27061 cataatcata aaacatacta gcaattggag aacgccagtc aagtattata agttcatctt
    27121 cataatcaaa tgcaaatcta ccaatataat acttagattc atcatccata tcttttagcc
    27181 taaaatctat tctagcaaag tatggagaat ctttaatttt atcaatctta tctcgaactt
    27241 ttaccataaa agccccatag ttatctattt gttttagccc ctgttcattt tgaaacattt
    27301 catgtgggtc tatttcacca cgatttttta ccatatacat tttagtatcc atataatcct
    27361 tgtcaagctt gtcaacaagg ttttcagctt tcttaaaagc atcatctaac ttattattta
    27421 tatctattag atgtgcaatc tcatctggaa aaaaagttaa taattctgta tcatgttcat
    27481 acatattgtg tttagaattg gatgcaatag tatcacaatt tatcacttgt ttatcttcaa
    27541 acatttaaat cctccttgta attaagtact tacactgaat cattatactt aattgtgctt
    27601 tgaagttttt aattattctt gctatttaac ttaaatatta actttaaaat tcatttttct
    27661 aaaactaaat ctttttatac ttataagcat aagtatattt acaaacaaaa tatgttgaaa
    27721 ataaaaacac tcttatatat aaattaattc aaacaaaaaa tatgtagtaa actttttatt
    27781 taatctacta ttaatttggt ctagtatctg tttcttctac agaaggaaga ataaattttt
    27841 gagtaggata cccaaattcc acaagtaatt ctgcttctgc aaaacccaac ttctttatca
    27901 tatctctatg acctgtatca gctttatcac cttctcgaaa agtagtaatt gagatatcag
    27961 tatctatcaa aaaatcttca ataactttct tcaaaaacac ttgtgcaatc tctgaattac
    28021 gatactgtgg atgtattcca aaaaaatcaa tgcttctagt ttctctatta aacaccatat
    28081 ttgcaatagc tacattatta agttttaata ttaaagctct ttcttctaaa atatactgtt
    28141 ctaattgata tatatattct tcctcttgca aatttggaaa tccatctatg actaatctca
    28201 ctaaattcat ccataatggt atatcagaca tatttgcaag tttaatttct ttttccaatt
    28261 ctagtacatt ctttgaaaat aagttatctt ttttcaaaac aaatcttagc tgcaaaggat
    28321 aaaattcttg ttctttccta tatttatttg gagatttttt atacatagct ttaaatatag
    28381 aagtgaatgc ctgttgactt tcatatccag ctatcaatgc aatttcaatt attggtttct
    28441 ttgaaaatac caataatttt gctgcttctg tcaactttct tcgctttatg taatcatgca
    28501 ttgtcagacc aacagaagtt gtaaaagttc tatgtaagtg atattttgag taatgaacag
    28561 cattagcgac tatatctaaa tccagctttt ctgataaatg ctcctctata tagtcaatgg
    28621 cagttatgac attaattact ttataagaca ttgcagcttt cctcctttta tccatgcaac
    28681 attatatcaa ttataactta gttatattta aattatcttt ctattgattt gttttatcgt
    28741 gtcttgtaga gagtgtaaaa tggcgtattc agtgtcaatc aatgtgccgt ctacataaaa
    28801 gacgatttct ttgtaatcca ttaatttctc cttaaattag cctaatcgaa aatcactgtc
    28861 caagtgtctg tacgacatgc tttaaagcac attttaatct gttcttctgt atttttttct
    28921 gtggcgagtt caggaagttc attttcggag aaataagaaa actccgtagt ttctatattt
    28981 tctttaaatt cacccccgat aacggtacaa agaacaaata ttttgcatac tccgtaagcg
    29041 tatataggta aattgtgttt cgcgcggtct tgaattgcaa taatcctgtc ggcggttata
    29101 tctaatccag cttcttcttt gacctcttta atagtatttt ccttgactga taaatttact
    29161 tcaacccaac cgcctggaag cgaccacttc cctgtatttt ctttaacaag aagtattttt
    29221 ccattttcaa aaatagcagc tctagtatca agttttggag tttggtatcc actttcattg
    29281 caaaaaagat ttcttacttt ttccgtggat atgtcagttt tatatgctat aatttcggcg
    29341 gaaatttctc gtattctctc ataccgctct ctgtcgtata tatctttccc ataagttatt
    29401 ccagcttgag cgatgctttg caattctatc gcccattcca accatttttc gttcataaaa
    29461 tcacctcccg ttttccgaag aatatattat aattagttac tgattagtaa aatctatttt
    29521 taactactcc tgttttgtat gttgaacata ttctataatt gtaccatctg gatgtctcaa
    29581 tgtcatatta tatcctgtag gaactttttt cttatctctt actacaactg ctccattatc
    29641 caataggtaa cttctccatt catcaacaga atcaaccata aaagtagatt ttgtttctac
    29701 aaatggttta agagcctcat cagaaccaga taataataaa acatttccta tttgtgctag
    29761 ttccaaccca acttctttat aagaaaaacg caaagaacat ttttctttta aaagattctc
    29821 ataaaaaggt atggcctttt ctatatcttg aatataaaaa cgtgttaata tatcgtatat
    29881 tttcatattt tccccctata tctctatatt atttataaag tatatcactc tacaaattat
    29941 atagcaataa attaagcatt accttttgag cattttaagt gcactttcac aatatgtttg
    30001 gggtacatca gaatacataa attaaatctc tttgatatta tttagtataa aatttataca
    30061 gtaaatgtcc ttattatcaa ctaataaacg agaatctttt actttctata taatgggtaa
    30121 catcattttc gtaaggtatt cactagctgg acgattgata tcatttaaat attggtaata
    30181 tatatcgcca tgaagtttat atccattact atttgcccat gaaaataagt cctctaaagt
    30241 agggtcttgt aattcatagg gccctaaatc aattgcagtt attattttct gtgaaggaat
    30301 aattctttgt aaaatttcat ttttcccatc tatataagtg gccacaggaa accctacttc
    30361 aacatccaat tctgctaaat ccatgttatg aaaacataca attggtgctc ctgaggataa
    30421 gattcctctt tcctccaaat atttagaaat tttttcgaga ctttgctttg aaaactctga
    30481 aaactctacc acaaagtcaa ttgtttttcg tattgctaaa atacaatact cagattgttc
    30541 agctattgtt atattagata ttctagtcat tttattcctc cattacttta tttttacctc
    30601 taatcctttt ataccaaatt acacaaaatt tcgctgtata aataattatt tcatatgaat
    30661 ataaaacata gcctccttcc atttcttata ttgaaagtat aacaaaaata atcttatgat
    30721 gtttgtcaca cagtgctctt atagtagtac atatttaaaa gacttgccta attttaaggc
    30781 aagtctaaaa gaaatcttct ttctataaaa aatttataca aacaattcaa atatcgaaat
    30841 ataagctact atcaaactat aaatttaaat ttactaatat caaatatcaa aatttaaaca
    30901 agtttcaggc tcatcttcta ttcttgctat tttaaactta catgcaatta ataccagaga
    30961 tactattgga tttataatat ttaacaaagc atatggacca tatgttagtg cagatacacc
    31021 taatgttgct gttatataag ccccgcatgt attccatggt actaatggag aagttaaagt
    31081 tccactatct tctaaagctc tcgacaacat cttaggatgt atacctctct ttttatattc
    31141 cttattatac attcttcctg gtataactat agatagatat tgttcacctg ctattatatt
    31201 ggtaattgta caagttacca tcgtacataa tactgttcca aatacaccct tagctaattt
    31261 tagtaaagaa gatgctacta cttcaagcat acctgttttt tctaaaatac ctccaagaga
    31321 taaagcacat attgttaaag acacagtgga catcatattc ataattccgc ctttagataa
    31381 taaagcatct acctcttgta tttgtgttga cgaaacatat ccattttgtg ctgcatctaa
    31441 tatcgtattc atagattctc cttgaaaaac tactgcaaac aatactccca atacagcacc
    31501 tactataagc cctggtatag caggaacttt aaatacaact aatccaatta ctattgccgg
    31561 agctaataat aatactggtg ataaagtatt aaatgtagaa tctaatgtag acctaattaa
    31621 ctctatctgt tttatatcta gcgcttgacc tgaatacttc attccgatta caccataaat
    31681 tactaaacaa attaataatg atggtatagt cgaatatagc atatatttta tatgtgtaaa
    31741 taaatctgtt cctgccattg ctggagctaa attagtcgtt tcagataaag gtgatagttt
    31801 atctccaaag taagctccag atattataga acctactatt agtccaactg gcatacctaa
    31861 actttgacca attcctaaaa gagctactcc tactgtaccc attgtactcc aagatgaacc
    31921 tgtcgcaaga gatactattg cacagactag tgttgatgct actaaaaaaa catttggtga
    31981 taaaattttt agtccccagt aaatcataca tggaactact ccacttacta tccaagttcc
    32041 tataagtacg cctattataa ttatgattaa gattgcctgc attgccatct ttatactatt
    32101 aaacatactt aattctaact ctttccattt aaatccaagt ctatatacag ccacaagtcc
    32161 tgccactgct gcagctccta ttagtggtac atgaggtgat gaaccatata ctaatactcc
    32221 tagacttaaa aataatatca aacaagtaat tggtatcatt gcatcaaaca gacttgcttt
    32281 tctacattct cgctctttca taaaataatt tcctcctaat tatttaaaca ctacactata
    32341 attttttttg aaacatattt actcgtacta gatgacatga taccaccata agttaataaa
    32401 aattctacac tgtcacctaa tttataatca tattttgtgt tgcttacatc tagtatcatg
    32461 tggtctgagc ttgcccctaa tatttttata tcagtgtcta ttggaattaa agagtcaata
    32521 tttatatcct gtttaccgat ggctataatt gcccttttta agattccttt atcttcataa
    32581 acaggcttat ttctaaaagc atcaaatcct atttctccaa taggaactga tggcttttct
    32641 ttacattcaa ctacttggca tattaattta aatgcatctt gatacgttcc atttatattt
    32701 tcaccgtatg ccgtttctct accaagtaga atagattcgc ctattctcag attagtaatt
    32761 ccttctggca tattatcatt tgtcatcaga tgtatagaac ttgaattgcc tccagatact
    32821 atttctagat ttattccaaa tttgccttcc attttttgtg ctatacttgc tagtctacct
    32881 atattttctc tagagggtat aatagctcca taacaagtta aatttgtagc tataccaatt
    32941 attttaatat tttttaattt tattatctct ctcacattat aaaaaaaatc ttcttcataa
    33001 aaataccctt ctcgtaaatc tcctaagtca aacataagga ctatcttatg aataatattt
    33061 ttactttcac ataattcatt taatttcttt attgttccta actctgagtt aaaactaata
    33121 tcacaataat tgataacttc ttctaattca cttaacattg ggattctaat caatatctta
    33181 ggtaaattta tgtcttgtaa attttttaag ttttgtattc ttgaatctgc tatatggtct
    33241 attccttcac tacatattgc ttttactact tctttttgtg cacaaaatga tttagtgaca
    33301 aaagatatct taatatttct ttcgtgacac atctgcgaaa ttaattttgc attatgcttt
    33361 aacttttcta tatctatttc taacctagga tacataatct ttcctcctca aaattcaaac
    33421 tttttaatag gagcttaaga gatgcttctt catatttttt agataaaaca cccatagatg
    33481 ccataatata atcattatct atcaatatct ttgttttttc ctttggaaat agtaattcat
    33541 tatttttatt gtatttaagc atattatcta atatttcaat tctactaggg agcctagtaa
    33601 gtgctccccc tgtaccaact atatatctta cttctgttaa atctttccct tctgcaattt
    33661 tgattttccc agttgttcca tagatatttc ttatttttcc tgaatgtctt aacatagctt
    33721 taacaacagc ttcttttgtt aatctctcaa caaataatat ttcttctttt gactttggta
    33781 ttggaggata attctctatt actgcatcaa tatctataga taattcactt tgtaagtttt
    33841 ctttacctat cacttccact atattcttca tatttacata tacacctaaa tcaccctcaa
    33901 cactcctctt tgcagtaggt tcaggattaa ctaaaatttt attaatatag tcacttccat
    33961 ctgtaacaga atgtacatca gtagttgctc cacctacatc cagagttaat aaatctccaa
    34021 tatttttata taagagttta gaagcttcca ttacagctcc tggggttgga gtaatatttt
    34081 cattaaccat ttctttaatg tatttcattc ctggtgctgt agttatgtgt tcttcaaata
    34141 cactttgaat gattcttctt gtaggttcta tattcaataa atctatttta gggtatacat
    34201 tttcagttat ataaagctta taattagtat cttcaaatat taaccttact tcctcttgat
    34261 tttcaatatt cccagcatat ataactggta tgcctagatt catagaagct atcatttctg
    34321 cattataaat agctgtatct ctttctccat aatcgactcc accagctatt aaaattatgt
    34381 ttgggtttat ttcttttatt ttatttaaat cagttctttt aattctacct gatgttactt
    34441 gacgaataac agctccagcc cctagggcag cttctttagc tgcttttact gtcatatcat
    34501 agactaatcc atgaacagtc atttttaatc caccagctgc actagaagtt gcaaacatat
    34561 cattatactc tatattatct acagataatt tattagctaa atccttaata gcacctgata
    34621 aaccgtttct tacatcgcca ccttcaaaca ctgttgttgg agcttgacct tgacccaaaa
    34681 atacaggatt gttagtatca atattatgga aagcgtttac cacagtagta gtgcttccaa
    34741 tttcagctac caacacatct atttttaagg aaactttgct attttccatt atttattatt
    34801 catttctctt cttttttcaa ctaagaaagt ggctacatgt atgcccttgc tgtttcttcc
    34861 aaatccagca tctattcctt gttcaacggc tatttcaggt gttacttgtg tccctccaca
    34921 cgcaatcatt actttatctc ttataccttt ttccacacaa taatcgtgta atttcttcat
    34981 gtttttataa tgaatatcat catgacttat tattgtagat gcaagtattg catctgcatt
    35041 cagttcaatt gctgcatcta ccaatttttc tacaggtaca gaagtcccta gatagtggca
    35101 ttctatacca tatttttcaa taccaccatg ttttatatct attatttctc ttaaaccaac
    35161 tgaatgttca tcttctccaa ccgtagctga aactattttc ataggttttt cttctatatc
    35221 tgctcttatc tcttcatcag ttaatacctt aggttcttct ggtattacta aattttttaa
    35281 atctatatca aatggaactc ttcctttcac ttctatccta gtcccttcag attcatgcat
    35341 aacctctcta cttataacct cagtttcttg taagttcatt tttttgacaa actccagagc
    35401 tgcaaattca gctgttcttt tatccgtagg aagcatcatt gttatcatta caattccatc
    35461 tgcacaccat tccatttcag gcttaatctt agttccattt ctatattcat ctgattcttt
    35521 taatctattc tctacattat caaaatcgtc aagctcatca atatatatta ttttatctgg
    35581 attttcaaat gtacatccat ctattaatat tgatggatta tctatagcat ttttatcgta
    35641 ttgggctaca ttattgtatc caaagtgagc agttacaggt gctaaataat cttcttctct
    35701 ttcataaaca gtaccttcac caactcctcc ttttattttt ctagatattc catcaccatt
    35761 tctttctgga taatttcctg agtcaacaaa gaatccttgt tctacagatt caaaatatcc
    35821 gccaacttca aatatttctt ccatatataa tacagctctt tctttaagtt cccttgcttt
    35881 ttctcttaat tcaccgacat ctttaagttc tatcatatcc ataagaccat ccataccaac
    35941 taaagcttgc tttgctgtat cacacgcttc tatattatat atatgccaag gtacatttct
    36001 accttcatca ggtgttatag ttgattgtat gtcagctcta gttaatactg atgttaataa
    36061 gtttaataca tgagtaactg ttgcctctct agttgatgat tccatatatt tagtattcat
    36121 ttgagctctc atcttataat cactaaataa ctctctaagt gcaacagcat aaggtaagtc
    36181 tattcttaaa catggtgctg gaggtgctgt tggtggaact gttgataagc atatattact
    36241 tttatctatt cctatttttt ctgagaatat agagtttaaa gcgtgttgaa ccataagttc
    36301 aggcattacc ttccatgctt ctctagcagt agcattagca ttatgcgctc catctatttg
    36361 agctatatta gcaaaagcca ttattttttt agcttcacaa gcatctacaa aagaccttat
    36421 catatttata tttctatata gaacattgta ttgtgggtct tggtgtgctc cattgactcc
    36481 ttcttcagca aacattactg ctacttcagg accagcaaca ccacttacat acgaatgata
    36541 attaataggt ctaccaactt cttcttctat aaaatcaaga gcctttcttt gagctcttac
    36601 ttgttttctt gttattggaa ctccacctat cccttgggga gttccttcta taagcccatc
    36661 aaaatgtgat tgcccagcag ttctaataac cattaaatgg tcagctccat gatgagctgc
    36721 catcctcatt cttcttatat catcctcaaa tctaccagaa gctatttcag tagtaataac
    36781 tgggcttggt tgagggtcta tattattgaa atacttagat gatggtagcc ccacactgtc
    36841 ttttaaaggt tttgtacagt ctctatattt aaaaggtccc atttcaagat tttccaaatg
    36901 ctctctccaa acccaacctc ttctttttgg tttatattta tccaaatctt ttaatatata
    36961 gtctatatcc aattttttat tttcctttaa catgattcta ccccttctct aaataaacat
    37021 gtaacttcat cccaatattc accattaact aatagtaaac ctgcttctct aactgttata
    37081 ttcttagatt tagccaactt ataaactaca tgccctgctc ctttacctat aagacctctt
    37141 tccataaccc cttccagaat aggttttact tcaagagaag aaaatcccat tcttaacaag
    37201 acagaccttt ctattgatgg tgttgtattc tttttaccaa gttctatcat tggttctact
    37261 atctgggtag ctaaactcca aaacctttct tttaactcat catcactcag ttcttgaaga
    37321 tgttttcttc taacttcaaa atcatcctct ctttttttca tgaacattcc tccattcgta
    37381 ttgatttaat cgaattttcc gaatttttat tttacattaa atcaaaaaac aacaactact
    37441 ataaactatt taatacatca ataacaaaat ctcttgaact ttttacttct tccattaaaa
    37501 attctatatc tcgattactt agcttatcaa tattatcaat gtgattattg gatatacaat
    37561 tttttataaa tgactttctt atcttattca tatcaatatc aacacatttc aataattctg
    37621 gtctttctgg taatattaag tttactcctg caatttcttc tcttggattt ccaaatttta
    37681 tatctattcc attttctctt gcaaaagtaa gttgtggaca tatatgttta cctgcacctg
    37741 tatattctgt ttcttgtacc actataactt tatcctcatc taactcttga gctaaagaga
    37801 atgctgctgc taaagatgta tttcctgctg gacctctttc tataccttct agctgtgcca
    37861 ataattcagt tgtataaaaa acttctcctt gttttactaa cacatatcta tccatatatc
    37921 taagtggtct agctgcagac ctaggtacat ctgacctatc aggccatgta cagtatggta
    37981 taccaaaacc tgtatgtcct gttgtaaatg attttttatt aaattgatta tcgctagcca
    38041 tgtgtaaccc ttttaaatct acacttgctc ctactacttt agtattaata gaaccagctt
    38101 ttatcaatcc tcttgcagtt ccagtaagat taccaccacc tgcatttgta caaacaacta
    38161 tatcagggtc ttttttatac ttttctctaa attgtatagc taactcataa cctaaagttt
    38221 ctacacctgc aattccaaat ggtgaatata atgatgcgtt aaaatagcct gtttcttcta
    38281 ataaaactaa gaacgtataa aataactctg gtccaacagt taactgaaca acttctgctc
    38341 catatgcttc acattttctt gctttttcta taatttcagg ctgacctaca ccattagaat
    38401 cataacactc ttgaactatt atacatttta atccttgcat tgcagcttgg gatgctacag
    38461 ctgcaccata gttaccacta gtagctgcta ttacaccttt atatcccatt tttttagcat
    38521 gatatactgc agtagctgct cttcttgctt taaaactacc agatgcattc attgcttcat
    38581 cttttacaaa tattctagct cctttacctt ctttagcaca ttttctagct aatttagtca
    38641 aatttttaag ctctattata ggagtatttc caacagcata ttgtgattga attacctcaa
    38701 tatcttgtaa tgtatatcct gtttcactca tcatcttctc ataatcaaac cctattcctt
    38761 cttgttcaaa agaagagtaa tccaatccaa ttgcattttt cattatttcg ttatttcttc
    38821 ccataacagc ttgataactc atatctttca ttgatgctga attattcatt agatacacct
    38881 ccaaataata tttctttagc ttggattcct atttggagta actctggtat acatttacca
    38941 taatcatgag tataatatgg gtttacttta agtaaattac cttttacaag tctacctgta
    39001 attgttttaa cttctactaa gtcgcctata gaagcatcac tttgtataaa acctttaacc
    39061 cacatttcta aggatacttt cttagtatcc tctggaactt gtggagctct ttcttctgga
    39121 gtcaatacta tattgtgtat aataacccaa tcattaattt tagcatccat tttttcatcc
    39181 ccttattaat tgactattgt cttactaatt aatttactgt tattttaaat taactattat
    39241 tttaccaatt agttagctgt tatcttacta attagccttc gattcttctt ccaattcaac
    39301 ttctatctga tttcttatat ctcccattat tgctctaggt actggtatat ccaacatagt
    39361 tttaagacct ggtctagcat ttattattaa tggaatagag tttactatca ttgcataagt
    39421 acctactcct ccaggtattt caggatttat ttgtaaatca atgtttggta taccttttat
    39481 tgatacataa tcgcctgttt taatgccttc cgcttctggt attatctgtt gtggatgctc
    39541 catttcaatt aaaacttctc catttacata tccatatcca cattgtctac aaccagcaac
    39601 atttccagcc aatgcctctc cgtacttaga ttttctatct actgtagtca ttattggttc
    39661 ttttgtttgt tctatcttat ctagtctcca tcctaatcca tccgctatca tatttattga
    39721 ttctacaaaa cctacatgac ccgctatagt cttatcttct acacctttta taaattcttc
    39781 tcttgttgct ccaacacctt gttctaccat aacagacttt ccaaatggag ataaatcatt
    39841 tactctctta gcttttatac tagtaacttc ttcacaagta cctgaaagtg ctaatactag
    39901 taaatctaat acaaaccctg gatttattcc tgttccaagt atacttactc cattttcctt
    39961 tgcaacttca tccatttttt tagctaaatc cacatcatca gcttgtggat atgccatttg
    40021 ttctgctgtt gatataacat ttatttttct atttaataag aatagtattt tatcaaaagc
    40081 ctttttagta aatgaatcag ttgccaataa aacaacatct acgctttttt ctctaaaaac
    40141 ttcttcataa tttttattga taataacttc tggtctttct cctctttcaa ttcctaagac
    40201 atcatacata cttttccctg aagtacttct actacaaaca gatactattt ctataccttc
    40261 tttttttaga atcatattag ccatacctat tcccatagct ccaaatcccc atattcctac
    40321 tcttactttt ctcatatata caccctccta aaaatttata tattctcaat aattagttaa
    40381 agcataaatc gtgccaactt taaaattaaa ataaatattt attagagtgt tgaaactata
    40441 attactttat tacgtatttt cataattaaa aaataatatt tcttctgaaa aataaaaaaa
    40501 agagcaaaat attttgctct tttataataa ttttgctcct ttattttgct tttttacttt
    40561 actttgattg ttcctctatt ttgctctttt ttatttataa tattttgata ttatattttt
    40621 ttattttata ctgtaaattt tgcctattca tacctaaaat tgatgcagct ttagttatat
    40681 tgtatgatgt cttttctagc gattcatata tatatttttc ttctatctca ctaataatct
    40741 tactaagtgt tttgtcctcc aaatcattta aagaataatt tttactctct ttattctctt
    40801 taagattgta atagtcattt aaattaatca tactcgtttc tatttgtacc tcgtcttcca
    40861 tcatactcat agtacaataa attatgtttt ctagctctct tatgttacct tgccaatcgt
    40921 aattttttaa aattttaata gcttcatctg aaatgcctat tactctttta tttaactttt
    40981 tattatattt tgaaataaat tttttgcaca atagaggtat gtcctctttt ctatctctaa
    41041 gaggaggaat ttttatatga attatgttaa gcctataata taaatctttt ctcattttat
    41101 gctcatttaa aatgaccttt ggactttcat ttgtcgttgc aataattctt acatctatag
    41161 gtatatcctt agttcctcca actcttctta cataaccttc ttgtaaaacc cttagtaatt
    41221 tagcctgtaa cataatgggc atagaattta tttcatctaa taacaaagtt cctttgttag
    41281 cttgctcaaa taagcctggc ctatctattg cccctgtaaa tcctcccttg ctagtcccaa
    41341 aaagtatacc ttcgaatagt gattctggta aagctgcaca attttgtgct ataaatggat
    41401 agtttctcct attacttcca tagtgaattg actggctaaa taactctttg cctgttccag
    41461 tttcaccata tataaatact gaagcatcag acttgcaagc tttcttagat ttttctattg
    41521 cttctttaat caaattactt tcccctaaaa tatcatcaaa ttcatagccc tttttttgtc
    41581 tactactaac cttattgact aaattttttt tatagtttct atttagtaaa ttttctatag
    41641 ttgtcatatt ttttgatatt tcaagtgccc ctacaaactc tcctttaata tcaattggta
    41701 ttgtagtatt aattgctgtt atttcttttc catttttatc tagatatttt tgaatattgt
    41761 ctttgataat ttcccttttt tccaaaacct ctaatagtgt acttctgttt attttcattt
    41821 tgtttactat atcaaaaaat gaattggtta aaatatcatg tctattcatt ttttccattt
    41881 tttgtacaga attattgtag attatagttt tcccgtcttt attaacaaaa tgtacccctt
    41941 catcaatatt ttccaatatt tcattcatta ttttaaaaaa tatctcttgg ttcaactata
    42001 tcaccccagt acctaaatat ataaaaaccc aaaaaattaa taaaatattt ccattgtatt
    42061 tatcttatca tatatttatg atatttttta tgtacattat attaatatat ttttaagtat
    42121 atttaaaatt ttatttatat atctttcatt atggttatac aatagttgaa ttgtcattaa
    42181 atttgccaat agttt
//
LOCUS       DAMJVM010000025        41573 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.025, whole genome shotgun sequence.
ACCESSION   DAMJVM010000025 DAMJVM010000000
VERSION     DAMJVM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41573
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.025"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            149..1342
                     /locus_tag="PC523_001435"
     CDS             149..1342
                     /locus_tag="PC523_001435"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDF2474971.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            1547..2665
                     /locus_tag="PC523_001436"
     CDS             1547..2665
                     /locus_tag="PC523_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HDF2474972.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEIYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNVDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVNSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAVGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSALLKYKEAID"
     gene            2796..3041
                     /locus_tag="PC523_001437"
     CDS             2796..3041
                     /locus_tag="PC523_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HDF2474973.1"
                     /translation="MKEKVQIEKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            3028..3774
                     /locus_tag="PC523_001438"
     CDS             3028..3774
                     /locus_tag="PC523_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2474974.1"
                     /translation="MSQYSDFAFVYDELMNEVDYDGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKDYDIAGIDISDEMLSVAKEKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKQDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENIIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLKE
                     SGFKDIKSYGDFTFDKPKEESQRIFFVCKK"
     gene            4058..4933
                     /gene="hslO"
                     /locus_tag="PC523_001439"
     CDS             4058..4933
                     /gene="hslO"
                     /locus_tag="PC523_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDF2474975.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYTEEELLEILKEM"
     gene            5323..6123
                     /locus_tag="PC523_001440"
     CDS             5323..6123
                     /locus_tag="PC523_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2474976.1"
                     /translation="MESKTKHIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRKLGVKPQNVIILADIDKKEGTHYELMKKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREHKIKDDLYFIKPKYVKYIPRNERAIYKAMEFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            6650..8890
                     /locus_tag="PC523_001441"
     CDS             6650..8890
                     /locus_tag="PC523_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2474977.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNDIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFSESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYKSLI
                     NGYNIADLTSMQIDELVEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHRGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPNAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNICEGKKFKKEVLEYRFQGKNIIEVLEMSVLDAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIG
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIEMGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            9303..10838
                     /locus_tag="PC523_001442"
     CDS             9303..10838
                     /locus_tag="PC523_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2474978.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTVMTITGSILIALLALIFDFILGLVERFFQRKRKKKNRKKV
                     FAILGSLILIVTIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLKVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTENMFSKLQSEYKNKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNISDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVIVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            10854..11615
                     /locus_tag="PC523_001443"
     CDS             10854..11615
                     /locus_tag="PC523_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2474979.1"
                     /translation="MTTIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKRAVDKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNAPANQFVDQLLNIRKSIIDDENLKK"
     gene            11735..11986
                     /locus_tag="PC523_001444"
     CDS             11735..11986
                     /locus_tag="PC523_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HDF2474980.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLDTNDVVNWCKTKIE
                     SPNAIINRKGKNWYISIYDCIITVNAHSYTIITAHKEKK"
     gene            complement(12299..12949)
                     /locus_tag="PC523_001445"
     CDS             complement(12299..12949)
                     /locus_tag="PC523_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474981.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSEVKQLIQNDNKVFTFKVDNTSK
                     YPDALNAILNCDRLIIAFPLYVDAIPSHLLHFLEECEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWIQGIGIGAGEMFGSLESVPLGKGQKKDLGIALKE
                     LSKNILSNNINIGNSKKNNIFISPNFPRFLYKLIGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(12946..13449)
                     /locus_tag="PC523_001446"
     CDS             complement(12946..13449)
                     /locus_tag="PC523_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2474982.1"
                     /translation="MKLLIHDLTDEELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPTECVVKDYYSNMGKLINESDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKHINLEAIFYGNCITESEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(13498..14013)
                     /locus_tag="PC523_001447"
     CDS             complement(13498..14013)
                     /locus_tag="PC523_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2474983.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQNHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKIDVEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            14293..14820
                     /locus_tag="PC523_001448"
     CDS             14293..14820
                     /locus_tag="PC523_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2474984.1"
                     /translation="MHEQIDYCERKKIRTIDIKGENMRELLKWVSIINRYNTNYIDRK
                     LADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGV
                     KDKRTWHLYPTEKSLECYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDM
                     NEEAKLEAKENGRKI"
     gene            14804..16207
                     /locus_tag="PC523_001449"
     CDS             14804..16207
                     /locus_tag="PC523_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2474985.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIIVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(16567..16878)
                     /locus_tag="PC523_001450"
     CDS             complement(16567..16878)
                     /locus_tag="PC523_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474986.1"
                     /translation="MKKIIKSFTFWFFLIALFEISMHQIGQDSKSIILIGFNPILSII
                     SRIDSFFIFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(16934..17527)
                     /locus_tag="PC523_001451"
     CDS             complement(16934..17527)
                     /locus_tag="PC523_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2474987.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEIDDNGLLPGSRQHISGANYTVEIMKQAPVTIFIL
                     NILGKSLLENLSPEERFYEMANIQSIGASIQNMSLKAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLNDVTEWR"
     gene            18052..18282
                     /locus_tag="PC523_001452"
     CDS             18052..18282
                     /locus_tag="PC523_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2474988.1"
                     /translation="MRLDMDSMLYVLIYMTLLVSVIGSIFYFCKDLLNHKIKYVLIKN
                     IFVNRTTYSEERLPKQYIVFQKYDIWRYCSLF"
     gene            18339..18989
                     /locus_tag="PC523_001453"
     CDS             18339..18989
                     /locus_tag="PC523_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDF2474989.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVNSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAMVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
     gene            19191..19862
                     /locus_tag="PC523_001454"
     CDS             19191..19862
                     /locus_tag="PC523_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2474990.1"
                     /translation="MAKILVVEDEKRMQSIIVEYMQKGGYTCVTADDGVEALTILKSN
                     NIDLMILDVMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTGIPKNSLILGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            19859..20989
                     /locus_tag="PC523_001455"
     CDS             19859..20989
                     /locus_tag="PC523_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2474991.1"
                     /translation="MSSLRKIIEKLKNLDISNWIKQNFGLHSVRKKVFLLSKLSGALI
                     ILFYLVVEELSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLMDEADEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVVDKMRMEQVV
                     ENLIINAKKHVVYNGNLDLSITCENGLILFEIYNDGRIIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVKNRDRGVEFYFMIPIDE"
     gene            21437..22282
                     /locus_tag="PC523_001456"
     CDS             21437..22282
                     /locus_tag="PC523_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2474992.1"
                     /translation="MIKVEELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSIFGNEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMAIDKPKNL
                     KMKNSNNKVEVEFVYNGSRDIKIVDIGELESGITFKYDEILSVHSKEATLEEVFIKYT
                     GRMLV"
     gene            22282..23310
                     /locus_tag="PC523_001457"
     CDS             22282..23310
                     /locus_tag="PC523_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2474993.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSTSPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKEMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNVGFFEGIKILSQVLVQASILSIIMSLIGFGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYIQLKNAFFMNFSNELIYFVVCSLTIIVLFLLVKNIF
                     NKELVREG"
     gene            23313..24326
                     /locus_tag="PC523_001458"
     CDS             23313..24326
                     /locus_tag="PC523_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2474994.1"
                     /translation="MIGLRYQLKSIRKDKMCIISFFLPVVMAILINFMGTIDMSSLGE
                     IHFGIVKDTLPVRTEQWLKKYGVLSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVL
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEILPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGVPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMLYIILDKKRKLS
                     FSR"
     gene            complement(24859..26109)
                     /gene="cme"
                     /locus_tag="PC523_001459"
     CDS             complement(24859..26109)
                     /gene="cme"
                     /locus_tag="PC523_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HDF2474995.1"
                     /translation="MENKNINSTWKKRVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTICSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINGSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVMSINNLIIVAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(26195..26941)
                     /locus_tag="PC523_001460"
     CDS             complement(26195..26941)
                     /locus_tag="PC523_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2474996.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELSLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAICLVNQYLKQLEIEKSN
                     AEEALTIVTQILNGHTPETDKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(27056..28078)
                     /locus_tag="PC523_001461"
     CDS             complement(27056..28078)
                     /locus_tag="PC523_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDF2474997.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKIIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSTGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGAGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIIE"
     gene            complement(28078..30498)
                     /locus_tag="PC523_001462"
     CDS             complement(28078..30498)
                     /locus_tag="PC523_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2474998.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLKPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEVKNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDEFLKILLDQGNLSTFGTNLVNNILSKTRHLIESRLGHLSLYREI
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGIEGGQLVYQGNLEGLLQSNKSLTGQYLSGKYSMPN
                     RTSTKNITHIDEIPCITVQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQTNSSESEIGDEDDYAVVETIADRLIGTKYISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSDCHGKRFNDESLSIKYKNKTIHDILNMSISEAVNFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRVKLAKEIGKKRNGNILYVLDEPTTGLSLYDTAK
                     LIQLLDELVANGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(30584..31504)
                     /locus_tag="PC523_001463"
     CDS             complement(30584..31504)
                     /locus_tag="PC523_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2474999.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLFLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTITDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNVNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
     gene            32110..33024
                     /locus_tag="PC523_001464"
     CDS             32110..33024
                     /locus_tag="PC523_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDF2475000.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSTSTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSENS
                     NTEILTGNSGIFKNIEKYLDVTINNNQKTMKYKHRDELFKNSNTNTYSRINEEHIDKG
                     YDSIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLNYESPESIHEIIIRKL
                     NSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEPTILF
                     GYEFESYLSEESSLSACNYGDMCYEDMY"
     gene            complement(33226..33846)
                     /locus_tag="PC523_001465"
     CDS             complement(33226..33846)
                     /locus_tag="PC523_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2475001.1"
                     /translation="MFLINILLVDDHMLFSKSLSIALEEYEEIEQFYSTQDIHSLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLIDSLLKVYQGGTCFPSENTEVIIEDLTSMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIQMGYISPVY"
     gene            complement(33818..35401)
                     /locus_tag="PC523_001466"
     CDS             complement(33818..35401)
                     /locus_tag="PC523_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2475002.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTKTYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLVLDSWYLLLSFVATPIGTIIFKILSTVIAYVS
                     IKFIFLFLFQGSGYKFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFF
                     YIIFYHWKRVAFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRSIFLFISILVISLVVLFASGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPEVQDIIYETLNNLNTCIRKQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNINDNGVKNTSFKTPLDITKITFDTIKHKGIISIKEQVENLGGTISISDNIPHGV
                     CVQITIPMKGDVSYKYFVS"
     gene            complement(35741..37768)
                     /locus_tag="PC523_001467"
     CDS             complement(35741..37768)
                     /locus_tag="PC523_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2475003.1"
                     /translation="MNIFYMAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKVM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENILTCSGAFIIGLVLGALVHKSIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAISNGKFLYKTSLIKLIRYEKSTEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWISIGFYQIGLITMIIIVIGTVLFINSFLPYIMQKSKKNKRAFYTGTK
                     IITIPNFIYRIRSNSKTLIMLTLLSAATLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYMSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGASKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLIKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYLLLINGKETPVTVRETSLNNAISFANSVGTLIVNDSIYEQIAQSKFPMTSILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIASG
                     SILYFNNISAISDSTSDYKILQKMGYTNIQIKKIIKKQVLPFFNIPFLLGIVDCIFAT
                     LVYKTALMQNLLGNSLSQYIPVIIAIFLTSVVYLIYYIFTIKSCCKIVLKK"
     gene            complement(37781..38527)
                     /locus_tag="PC523_001468"
     CDS             complement(37781..38527)
                     /locus_tag="PC523_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2475004.1"
                     /translation="MNTILKVNSITKTYKGQQPSLDKVSFNVSEGEFIGIMGASGSGK
                     TTLLNVLSTIDKPTSGDIFINNINLKSLNDTNAADFRKDNLGFIFQEYFLLDSLTTFE
                     NISVPLTLQKLSPDKIEKMVKSLAERFGIASQLNKYPNELSGGQRQRTSAVRAIVKQP
                     SILFADEPTGALDSNSATELLHSLQEANKELKTTILMVTHDAYAASFANRILIFKDGH
                     IIKELNKQQNNRHTFYELIIQEIAKLDTKR"
     gene            complement(38818..40479)
                     /locus_tag="PC523_001469"
     CDS             complement(38818..40479)
                     /locus_tag="PC523_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HDF2475005.1"
                     /translation="MNKKNLAILISGIMFFSSSSSIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIESSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKALNIKINKIVGNNIYEMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSVNFLNSRSIKKSYIVGGNKILSD
                     NAVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGISQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIENKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQTLPGSDILVCYYDEDDNEYDCKVVKDNVLYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTKNISLDKAKAIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIIDIDMDNDSDDDIDDNDNNHNNNSNTNDNSSIISAEKAKSIILDKVP
                     GGKIVKFKYDEDDKEYKGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            41078..41515
                     /locus_tag="PC523_001470"
                     /pseudo
     CDS             41078..41515
                     /locus_tag="PC523_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
BASE COUNT        15280 a         5040 c         6628 g        14625 t
ORIGIN      
        1 tattaattta ataaaatata ccaaaatggg aataaaaaat aaattttatg agaatttaat
       61 aactaataat taatatattt aggttataat aaatatgtgc gtaaaatcta ataataagca
      121 ataatacagt aaatttagga ggaataatat ggatacttta aaggaacttt ttaaaatagg
      181 aagtggacct tcaagttctc atactatggg gccacaaaga gcagcagaga gatttaaaaa
      241 tgaaaaccca gatgcagaaa gttttagagc aatattgtat ggtagtttgg cagctacagg
      301 gaagggtcac ttaactgact atataattga aaaaactata gcacctaaaa aagttgaaat
      361 tgtttgggaa gaagatatta taaaagattt tcacccaaat gggatgaagt ttgaagcttt
      421 agataaagat aaaaatgtaa cagctgaatg gactgtatat tctgtaggag gagggactat
      481 agcagaagaa ggtcaaagaa acagtaaaag caatagtata tatcacttag atactatgga
      541 tgaaatagta aagtggtgta aagaaaataa taagactttg gtagattttg tcttggaatg
      601 tgaaccaaaa gacataaaag attatataaa aactataaaa gatgctatga gaaaatctat
      661 tgatgatgga ctaagtacag atgaaataat accaggcaaa ctattattaa aaagaagagc
      721 aagtacattt tataatgcat ataaaaaaga taagagtttc tcaacgcttg tatatgcata
      781 tgcattagca gcttcagagc aaaacgcttc tggtaatata atcgttacag caccaacttg
      841 tggttctgct ggagtaatac caggaatatt ctttgcaatg caggatttct ataattatga
      901 tgacgaaaaa ataattgaag cactacttgt agctggaata ataggtaata taataaaaac
      961 taacgcttca atatctggag cagaggtagg gtgtcaaggt gaagttggag cagcttgttc
     1021 tatggcagcg gcggcagtag catacttaaa gggtggaact atagaccata ttgaatatgc
     1081 ggcagaaata gcacttgaac atcatttagg aatgacttgt gaccctgtat atgggtatgt
     1141 acaaattcca tgtatagaaa gaaatgctat ggcagctcaa agagcttatg atgcagctaa
     1201 ttatgcatta ttaactgatg gctcacattc tgtctcctta gaccaagttg ttgaaacaat
     1261 gaaagaaact ggtatagata tgatggataa atacaaagaa acagctaagg gtggacttgc
     1321 taaacatttc ttctcttgct aaaaataaaa ttttccaata ataattcccc tgttttgatt
     1381 tatgaaacag gggaattatt attaatggtg aattatttaa taatgcaaaa tatttttata
     1441 ttcatttagt tttttatttt taattagtat aacatgttat ataatatttt aattataaag
     1501 aaatatagaa atatttttta ataaattaga aattggagga gattacatga gagaggcatc
     1561 atatttacaa gaacaacttt ataaacatag gaagtcatta aatcctattg cagagattgg
     1621 gagaaaagag tataagactt caaagtatat aagagattac cttgataaat tgggaattga
     1681 gtatgaaatt tatttagaaa caggtatagt aggtgtaata gaaggtaaaa gacctaaaaa
     1741 agatattgct tttagagctg acatagatgg tcttcatagt gaagaagggg taaaacattt
     1801 atgtggtcat gatggacatg ctactatatt acttggatta attgagtatt taaatgataa
     1861 taaagaagac ttaaatgata atatagtatt tatattccaa ccagcagaag aaggtcctgg
     1921 gggggcaaaa gctctaatag atgaaggcgt gcttagaaaa tacaatgttg atgaaatata
     1981 tgggcttcat atatatccag agttaccaga aggttatgta ggtacaagag aaggctattt
     2041 tatggctcaa ataggagata ttgatataga aatagtgtct aaaagtggac atggagctat
     2101 gccacaaaat ggtattgatg gtatagtgat agcttcaaat tttgtaaact cactccaaac
     2161 tatagtgtct aggaatataa gtccaataga caatgcagtg ttaactattg gtagaattga
     2221 agggggagct agaagaaata taatagctga aaatataaag atggaaggca ctatgagatg
     2281 ctttagccca gaagtttatg agaaaatgaa gcttagagtt agagaacttg ctagagggtt
     2341 tgaattggct tataactgta aggttaatat aaatattata gatgattata ttgctgttaa
     2401 aaatgacaag gatttgtatc aagagtttgt agaggctgtt ggaagtgata ctgttgtaga
     2461 gttagagcca cttatgatat cagaagattt ttcttattat caaaaagaag taccaggatt
     2521 attttttatg ttgggttcaa gaaacgaaga aaaaggattt gtaaatggtc ttcataatat
     2581 aaattttaat tttgatgaaa agatatgtgt aaatgcattg aatgtttatt cagcgttatt
     2641 aaaatacaaa gaggcgatag attagaaaga taaattataa agattaaaaa gtagaaatac
     2701 ctaaatttaa aaaaagtgta gtgataaata ccccttacat atttgtataa atttatttta
     2761 attgacaaaa atacaaataa gaaggggtga tttttttgaa agaaaaagta caaatagaaa
     2821 agaagataac taatggcaag aatactaaaa tattgacaaa tgatgatata atgaaatatg
     2881 agatagcctc agaactaggt cttatggaca aagttggtga acgtggatgg gcaggtctta
     2941 cagctaagga agcaggaaga attggaggta tgcttacatc acgcaaaaag aggctgaaaa
     3001 agattgtgga ggagcagaat aaaaaggatg agtcaatata gtgattttgc gtttgtatat
     3061 gatgaactta tgaatgaagt tgattatgat ggatgggtta aatatataga agatataata
     3121 gatagtgaag gtgtaaaagt aaaaaatata ttggaattgg cgtgtggtac gggtaatctt
     3181 acaattccat tgactaagaa ggattacgat atagctggta tcgatatatc tgatgagatg
     3241 cttagtgtag caaaagaaaa ggctgaaaaa gaaggagtag aattagtttt acttcaacag
     3301 gatatatcag agcttgattt tgaaatatct gatttggact gtgtactttg tgcttgtgat
     3361 ggattcaatt atataactta tgatgatgat ttggaaaatg tattttcaaa gacatatgaa
     3421 ttattaaagc aagatgggat atttatattt gatataagtt cattttataa attagccaac
     3481 atattgggaa ataatatgta tggagaaaat agagaagata tagcgtatat gtggcaaaat
     3541 tactttgatg aagaagagaa tataattgaa atggaactta cattttttat taaagatgag
     3601 tgtggaaagt ttgaaagatt tgaagagata caccaacaaa gagcatatac tgaagatgag
     3661 atagttgata tgcttaaaga gtctggattt aaagatataa aatcttatgg agattttaca
     3721 tttgataaac ccaaggaaga aagtcaaaga atcttttttg tatgtaaaaa ataagatatt
     3781 tttggatata aaagaaactt attaaaaata agcattatat aaaaatataa aaaatgcagt
     3841 tttatagatt aaaaataaag tgatgatatt ttatgattta tgatttttaa tagttataaa
     3901 gttctataga ttaaaattag aataaaagaa ctattaccta gtaaaattaa aataaaagag
     3961 ctattaccta cgattaagat agataagtat ttatagagta gaattaagat agtaatatag
     4021 taaaatgaga taattaacga aatatggagg aatattgatg agagattatg tactaagagc
     4081 gacatcagga aatgggcaag ttagagcatt tgtcgcaact actagaaata cagtagagga
     4141 agctagaaga ttacatgaaa ctacaaaagt agcaacagca gcacttggaa gaacattaac
     4201 agccacatct ataatgggat taatgatgaa aaatgatagt gataaattga ctgtaataat
     4261 aaagggtgga ggtccaattg gaactattat cgccacatca gattctaagg gaatggtaaa
     4321 aggatatgta ggaaatccgc aagtagaggt tgaagactat ccaaatggaa agttaaatgt
     4381 agcagcagca gtaggtacag aaggtgttgt aaaagttata aaagacttag gtcttagaga
     4441 accatataat gggacttatc cattggtaag tggagaaatt gcagaagact ttacttatta
     4501 ttttgctgta tcagagcaga ctccatcagt agtagcttta ggtgttctta caaaagaaga
     4561 tgaagtagag tttgctggag gatttatagt gcaattaatg ccagatgcag aagaagagac
     4621 tattgctaag ttagaggaaa atgtagctaa attaccttct ataactaaca tgttaaaaga
     4681 aggaaaatca ccagaggata tattaaatat tgttttagat ggtttggagc ccaaaatact
     4741 tgatacctgt gaagttgggt ttatgtgtga atgttcaaaa gaaagagtaa aaactgcttt
     4801 ggttgcaatc ggtaaaaaat cattggctca aataatagaa gaagataaaa aagcagaagt
     4861 tggttgccag ttctgtaata agaagtatat gtatactgaa gaagagttgc ttgaaatttt
     4921 aaaggaaatg tagactataa aaataggtgc attgatatag tagtatcata gtgacatagt
     4981 aatgtcttac ttatatagga attaaaatat aatttattaa taatagatat atttctatta
     5041 aaaaataagt ttaataaatt tttaagctta ataaatttta agaattgaga agctttttag
     5101 ctgtaatatt tgcaatgttg cagttagaaa gcttttttat tttattatag ttcgtgtcaa
     5161 agattgtatg aaacaataaa atattgtaaa ataatttgta tatttaaacc tagttgccac
     5221 aaaggtttat aagatatttg aattgattat gcgaaattct atgaagtagt aaccctgtga
     5281 ctttaatcat gggagattca gaagaaggag aaaattttaa atatggaaag caaaactaag
     5341 cacataggaa taagtatatt aatattattg atgatatttt taattgcaaa gaattttaat
     5401 ggaaaacttg aaatacctat tcctgagagt aatagtagaa aatttaaatc agcagcagta
     5461 ttttatcctc aacatcagga tgatgaggtt ttatggggag gaagtgctat tgtagatgct
     5521 ataaaacagt gtggtgttga taatgtatat gtggtattag tatcagatgg ttctggagtt
     5581 aacgttttca aagctaacac aaaatttaga aacttaacta ggaagcaaaa ggaggaactt
     5641 agaaataacg agtttaaatc agctttgaga aagcttggag tgaaacctca aaatgtgatt
     5701 atacttgcgg atatagataa aaaggagggt acacattatg aattaatgaa aaagactata
     5761 ttggattttg agcataaatt taaaggtaat gtgacccata tagcccatca ttataagtat
     5821 gatgaccata taatgcatag aaagaatggg gaagttttaa agaaacttaa acgtgaacac
     5881 aagataaaag atgacttata ttttattaag ccaaaatacg taaagtatat accaagaaat
     5941 gaaagagcta tatataaggc aatggagttt gatgactata acaaggttaa gagtgcttgt
     6001 tatgaatata aggttgtaaa ttcaaagcgt aaaatgtatg gtataggata tatttcggca
     6061 catagttatt ttgaacatct tttaaatgac cctgaactta cgtctgtatt aagtgaagat
     6121 taatttctta taagtagtaa gttatgtttg ataagttgat tagagttttc tatagtttcg
     6181 aaggtattga aaatttagtt ttatttaaaa atttaaagat agaggttttt aatgtctggt
     6241 attttaaagt taagttgaat aattgtatag aattatttgt gaaagaagaa acagtgtaaa
     6301 acatagtatc ttatttatgg ttttaaaatc ataggtaaga tacttttttt atgtaactaa
     6361 taccattttg tgattgttaa cttaagagtt ttatataatt taacactcat atataatgat
     6421 gatatttatg tagaataaat gcaattacgc ttaaataaaa taactgtatt ttttatagaa
     6481 atcaaaccct acactatact accagataaa atctacatgc tataatttga cctgtgttta
     6541 taaaaaaagg agtaatctaa taaaatagta ttttatttat gtataactaa caactattat
     6601 tacgatttac tctaaagaat ttaaagaaaa ataatgcagg aggtagaata tgaacaatga
     6661 atttataatt ttaaaaggat gcaaagagaa taatttaaac aatatttcct taaaaatacc
     6721 aaaaagaaaa ataactatat ttacaggggt gtcaggttct ggaaaatcat ctattgtatt
     6781 tgagacaatt gcaaaagaat cacaacgcca actaaatgaa aggtttagta catttgtaag
     6841 aagtttttta ccaaaatatg gagaggttaa agctgattgt atagaaaatc tgtcaacacc
     6901 aattatcata gaccaaagta gacttggtgg aaattcaagg tcaactctag gtactattac
     6961 agatgtaaat tcttttttaa gagctttata ttcaaggttt ggtagtgagt atataggtaa
     7021 tgctaatatg ttttctttta atgatattga tggtatgtgt ccacactgcc atgggcttgg
     7081 aaaaaagcta gttcctaata tgaatcaaat tcttgatatg aataaatcat taaatgaagg
     7141 tgctatattg ctttcagggt ttggagtggg ttcttggcat tggaatatat tttctgaatc
     7201 aggttttttt gatattaata agaggatatg tgattattca gaagtagaat tggaaaagtt
     7261 tttgcatggt aaagcagaaa agattaagat agaaaatgct ggtcaaacta acatgacata
     7321 tgagggatta atggttaaat ttaatcgcct gtacctaagt agagagggag aaatatctga
     7381 agctactaag aaaaaattga gtaaacttct tatagaagat aggtgtccta tatgtgatgg
     7441 aagaagactt aatgaaagag tatataagtc tttaattaat ggttataata tagcagattt
     7501 aactagtatg caaattgatg aattggttga agttattaaa agtatagatg agccagaagc
     7561 acaaccttta attaaaggaa taatagaaaa gttaaacagt atcatagata taggtcttgg
     7621 atatttaaca ctggatagag agacatcttc tctatcagga ggagaatctc agcgtataaa
     7681 gatggttaag tatttaaata gtaatcttgt tgatttaatg tatatttttg atgaaccaag
     7741 tgtaggactt catccaaaag atgtgtataa attaaataat cttttaaaaa aattgcgtga
     7801 taaagggaat acaattatag ttgttgaaca tgacccagat gttattaaga ttgcagacca
     7861 tattgtagat gtaggaccaa aggcaggtaa gtatggtgga gaagttgtgt atgaaggcag
     7921 ttatgaaaat ctacttacaa gtggtacgtt gacagggaat gctttaagta aatttcttcc
     7981 tattaaagaa agtgtaagag agcatagagg atatttagaa gttaaaaatt gtaataaaaa
     8041 caatttaaaa aatgtatcta ttaagatacc taaaggcgta ttgactctgg ttactggagt
     8101 agcaggagca ggtaaaagta cgttgattaa ggatgaattt ttaaagcaaa atcctaatgc
     8161 tgtacttata gaccaaagcc ctgtttcagc aaattcaagg tcgagtttgg caacatatag
     8221 tggaattatg aacaacataa gaagcatatt ttcaaaaaca aatggcgtaa atgcttcttt
     8281 attcagttca aattcagaag gagcttgtga taactgtaag ggttctggaa tagtagaaat
     8341 gaatcttgct tttatggaga gcataaagag tacttgtaat atttgtgaag gtaaaaaatt
     8401 taaaaaagaa gttttagaat atagatttca agggaagaat ataattgaag ttcttgaaat
     8461 gtctgtttta gatgcaattg agttttttaa tttaaaacag attaaaacga aattacaatg
     8521 tattgaaaaa atgggtattg gataccttac tttaggacaa actcttgata ctttgtctgg
     8581 aggagagtgt caaagattaa agcttgctag tgaattaaat aaggaaagtt cagtgtatat
     8641 attagatgaa ccaacaacag gtcttcatat ggctgatatt gagaatttta taggtataat
     8701 tgaagaaatt gtagataatg gaaatacggt cataataatt gaacacaata tagatgtaat
     8761 taaaagagct gattggatta ttgagatggg acctgaagga ggaacaaaag gtgggagagt
     8821 aatatttgaa ggtattccaa aacaattatg taacaataaa atatcattaa cagcaaaata
     8881 tatagtataa taaataaaat ataataaatt tataatgata ttttaataaa tataatgggt
     8941 gtataaaatg ataattaaac ttaacagtaa taaataatgt gagaaatatt caaatttaat
     9001 taaataaagt gtcagaaatt tgtttaaatt ttagcatttc aacagttctg accttttttc
     9061 ttatttttat ggagaatatc gagaatggag tctctaatag ttactttttt tgtctacttg
     9121 caagtttgat taaaaaatgt ctatgcacac ttgtgacttt agtcatggaa ggctcagaat
     9181 ataatcttaa tatataattt gtgaaataat ttgattacga tttgctgatg tataaacaat
     9241 aaagattaac tacattaaaa tttaaatctt gttaagtaaa acatataaaa ggagagagat
     9301 gaatgataaa tgatattcta acattattat ctgaaagaag tgattttttt ataaagctat
     9361 ccatagaaca tttgatgatt tctattattt ctataataat tgcgattgtt ttgggtggta
     9421 taattggtat tattgtaagt gaatttcata attcttctaa acctatattg ggaactatca
     9481 attttctata caccattcca tctatttcta tgctaggatt tctgatacca ttttctggaa
     9541 tagggaatac tactgctgtt attgcactta ccatttatgc attattaccc atggttagaa
     9601 atacatatac tggaattacc aatgtggata aaaatattct tgaagcagct agaggaatgg
     9661 gaagtacaga ttttcagata ttgtataaaa taaaacttcc attagctcta cctgttatta
     9721 tgtctggtat tagaaatatg gtaattatga caattgcatt agctggtatc gcttcattta
     9781 taggtgctgg aggcttaggt gtagctatat atagaggaat cacgacaaat aacacggtaa
     9841 tgacaattac tggaagtata cttattgctc tattagcttt aatatttgat tttattttag
     9901 ggctggtaga aagatttttt caacgaaaaa gaaaaaagaa aaatagaaaa aaagtttttg
     9961 ccattctagg aagccttatt cttatagtaa ctatctttac atcttattta tttaatcctt
    10021 caaagactca aactattcac attgccacaa aacctatgac tgaacaatat attcttggag
    10081 agatgttgga tattttaatt gaaaaaaaca caggtctaaa agtggaaatt acacaaggtg
    10141 taggaggagg aacatctaat attcagccag ctatggaaaa tggagaattt gatatttatc
    10201 cagagtatac aggtacaggt tggaatatgg tacttaagaa aaatgaatta tatactgaaa
    10261 atatgttcag taaactacag tcagaataca aaaataaact taaaatgcaa tggactggta
    10321 tgtatggttt ttctaatagc tatgcattag cagttagaaa agaagttgca gataaatata
    10381 atttaaaaaa tatatccgac ttaaaagatg tatcttctaa attgacattt ggagctgaat
    10441 atgactttta tgagagagag gacggatata ctccattatg taatacatat ggactgaact
    10501 ttaaagatac agttgattta gatattggat taaaatacca agctatcaat gaaggtaaga
    10561 ttgatgtaat gcctatattt acaactgatg gtcagtatag tgtttcagat gttatagttc
    10621 taaaagatga taaaaatttc tatccatctt atcaatgtgg aaatgttgtt cgtttggagg
    10681 tgttagagga aaatccagag ctatcaaagg tatttaagat ggttggagat attttgactg
    10741 ataaagaaat ggctaaaatg aattatgacg tagaaaacaa caatttagaa cctagagaag
    10801 ttgctcgtga gtttctgaat aagaaaggtc ttttataaat tggaggaaat gtaatgacaa
    10861 ctattataca atttaaaaat attaaaaaac aatataatga taaaactata atagataatc
    10921 ttaatttaga tatagaaaaa ggagagtttt tgactgtcat tggttcgtct ggttctggta
    10981 agacaacact cttaaaaatg attaatggtt tgattttgcc agatggagga aatattttga
    11041 taaaccaaac agatatcaaa aacgaggatt taattaaatt aagaagaaga atagggtact
    11101 gtgtacaggg aagtgttctt tttcctcata tgactgttga agaaaatatt tcatatgtac
    11161 caaatcttct aagtaaaaaa aataaattag aagtaaaaag agctgtagac aaatggatgg
    11221 aaatagtggg attgccaaat gatatgaaag tacgttaccc atcagaattg tcaggtggtc
    11281 aacaacaacg tgtagggatt gcaagagcct tagcttcttc tccagaaatt ttattaatgg
    11341 atgaaccttt tggtgcagtt gatgagatta cacgaaagca attacaaaaa gagataaaag
    11401 aaattcataa aaaaacagga gttacgatta tctttattac acatgatatt tatgaggctt
    11461 tgattttggg aacaaagact ttggtattaa atcatggagt tgttcaacag tatgatactc
    11521 ctgagaatat tttaaatgct cctgctaatc aatttgtaga ccagttattg aatataagaa
    11581 aatctataat agatgatgag aatttaaaaa aataaattta aatttaaaat caaattgttg
    11641 tgataaaagc tattgaccta gagttaactt caagttgtat attggtacta aataatagat
    11701 ttaattttgc aaaggataag gtgttttaat aaaaatgaat gtagaaaacg aattacttaa
    11761 aaacttagat agattgcata caacagagtt aggtgttgta agaattaaga aaaatctttc
    11821 tttggataca aatgatgtag tgaactggtg taaaactaaa atagaatctc caaatgccat
    11881 tattaataga aagggaaaaa actggtatat aagtatatat gattgtatta taacggtaaa
    11941 tgctcacagt tatacaatca ttacagctca taaagaaaag aaatgaaatg aattgcaaaa
    12001 tacaaaggtc aagagcattt tccattgttt gtagtaccta tgaagaagta tataaaataa
    12061 tattactttt gagtaataat ttaaattttg gagattgaaa aattgaatat actgatttac
    12121 atcagtacct tgttggagcg acttggattt tacaaaaaga atatgataaa tattagacta
    12181 gataatcaat gttgtagtga attattccat gtctagtgaa tataaataac ataaaaaacc
    12241 gtcaagatga atagtttatt cgtgcttgac ggtttttagt atttgttgaa gtaaaatttt
    12301 atttctttag tatatcactt ttagtcaaac catttgattt agcttgagta ttccactgat
    12361 gattacctat aagtttatat aggaatctag gaaaattagg tgaaataaaa atattatttt
    12421 tcttggaatt acctatattt atattattac taagtatatt ttttgaaagt tcttttaatg
    12481 caattcctaa atcttttttt tgacctttac ccaaaggtac agattctagt gaaccaaaca
    12541 tctcaccagc tccaattcca ataccttgaa tccatgtaaa gttgattttt ttacaccaat
    12601 gctctatcat ttgaattgcc aaatgattat gttcaccttc attgaatcca cagttaacta
    12661 ttgcatacac tggaataggt tttttatttt gtgtttttaa aaatttttca cattcctcta
    12721 aaaaatgaag taaatgagat ggaatagcat ctacataaag aggaaatgcg attattaatc
    12781 tatcacaatt aagtatggca ttaagagcat caggatactt agatgtgtta tctactttaa
    12841 atgtaaaaac tttattgtca ttttgaataa gttgttttac ttcggataaa aaatactgtg
    12901 aattattttt atttactttt ggactaccat ttatcattac tatattcata tggactcacc
    12961 tcttatatct tgtatatttt catagaaaca aacagagtgt tcttttatat tgaaatttat
    13021 agcatttcca tttactaatt tcgtagcagt ttctttttca gattctgtaa tacaatttcc
    13081 atagaatata gcttctaaat ttatatgttt gtcgtatcta cttttatgac gcatcatatt
    13141 atcttttatc acaaagtatg gaagaatata attaatactt ttatcaagta cgtttttgac
    13201 aaaagggcta aaccctccgt aacagcatct gcttattata ataattccat cagattcatt
    13261 tataagttta cccatgttgc tgtaataatc ttttacaaca cactcagtag gagttttaat
    13321 ccagcaacca aaacaaccta tacatttgtg gatttctttt gtgtttggaa gtatttttat
    13381 attgtcatca agtttgggta atagcttttc taattcttca tcagttaaat cgtgtattaa
    13441 aagcttcata aaatccatct ccttttatta tgtatgttta cttctaaaaa tcatatctta
    13501 taaatttttc agtaaatctt tacaccaatt aattacaaaa ttataatagt caactccata
    13561 aatcattgtg gcatacatat atgtatattg gtcaacacaa tgattttctt ctacatcaat
    13621 tttttgttta cttaatttat ttagaagtaa ttctacttca gttattagca tttttaagtt
    13681 tgtttcaatt atttctttgg gtagatactt gtaaaagaat atattaacaa ggtggttttg
    13741 ctttgtttta gaaaaattta taggtttctt taaccactca agaaatactt ctttaccagt
    13801 attagttatg gagtatagtt tttttaattt gctaccatct ataacttcgt gatagtgtat
    13861 atatcctttt gtttctaatc ttttaagtgc aggatagata ctgccaaagc tagcgtcaaa
    13921 aaagtacgag gtactctctg acattatttg ttttaagtcg tatccactta attctttttc
    13981 cattaaaaat cctaaaataa tgtattctaa catgttgcct cctagtatca attagatata
    14041 tatcaattcg atatatttat tattgcagat aataaatatt acgtcaagat gctttttaaa
    14101 ataagctaaa aagaattaga aattattaat aaagatgtaa aaaaattaat ataaaaggta
    14161 attaaatatc aatgaagatt taaaaatagt taaatcaaag taagagtcta atatataaaa
    14221 tttatagtaa ctttatggtt taaatataaa cctgtgtgct attgacaaag tataaaatta
    14281 gcagtaaaat gattgcatga gcaaatagat tattgcgaac gcaaaaagat aaggacaata
    14341 gatattaaag gagagaatat gagagagcta ttaaaatggg tttccattat aaatagatac
    14401 aatacaaatt atattgacag gaaattggcg gatattggaa tcaacaatag ccaatatttt
    14461 tatgtattac acatatgtaa taatccaggt attacacagg atactatatt tgagaaaatt
    14521 cttgtgaatc ctagtaatat aacaagagct ttggcaaatt tagaaaaaga aggttttata
    14581 acaagagaac caggtgtaaa ggataaaaga acatggcatt tatatcctac agaaaaatct
    14641 ttagaatgtt atgatgaaat actaaaaata acaaatgggt atgttgagga gttgttgagt
    14701 tcatttgaga aagaagagca agagttgttt atgtcaatgt taaaaaaagt tgcatttttg
    14761 gctattgata tgaatgaaga agcaaaattg gaggcgaagg aaaatggaag aaaaatataa
    14821 ggagccattg gaatatgaaa aagtttcaaa gttattaacc aagtacgcaa ttcctagtat
    14881 tatagcaatg ttagtaagtg ctttatataa cattgttgac caaatattta ttgggcaagg
    14941 tgttggtgta ttgggtaatg ctgctacaaa tgtcactttt ccgctaacaa caatatgtac
    15001 tgcaattgca ttattgttag gaattggagg agcgtctaag tgttcattgg aattaggtgc
    15061 taaaaattca gaaaaagcta caaaagcagc aggaaattct atatgcttga tggctgtttt
    15121 tggaataagt ttatttataa tagtatcaat atttttaaca ccactattaa aattctttgg
    15181 ctctacatca gaaatactac cttatgcaca cacatataca aaaattacat caattggatt
    15241 accatttctt ataatgtcca ctggaatgag taagttgata cttgcagatg gaagaccaaa
    15301 agcttctatg ttttgtatgt taataggagc agttattaat acaattttga atccattatt
    15361 catttttgtt tttaatatgg gaattgctgg gtctgcttta gcaactgtta ttgggcaact
    15421 tatatctttt ggaattagtg ttcattatat aaagaatttt caacatatta aattacataa
    15481 gaaaagtttt aaattagata gtataattta taaacatata tttgcactag gttcaagttc
    15541 atgttttaac caattagcaa tgactgtaac acagatagtt atgaataata cattggctta
    15601 ttatggggct aggtcagttt atggaagtga gattccactt gcctgtgttg gtatcattat
    15661 taaagtaaat atgattttta tatctataat tattggtata tcacaaggta tgcaaccaat
    15721 tatagggtat aattatgggg ctgaaaagta taatcgtgta aaagaagcgt ataagtttgc
    15781 agtgggaagt gcaactatta tatctgttat tgcattctta tgtttccaat ttttcccaag
    15841 acaaattacg tctatatttg gtaacggaaa cgagacttat tttatatttg cagaacatta
    15901 ttttagaact ttcttgttct taacatttat aaatggtgtt caaatagtat catcaacttt
    15961 ctttacatca attggtaaat cagtgttggg actttttact tctttgactc gtcaaatttt
    16021 atttttaatt ccattgatag tgattttacc aatgtttatg gggattgatg gagtaatgtt
    16081 tgcaggacca atagcagatg cagcggcggc aatggtatgt atatactttg cagttcatga
    16141 aatgaaatca ttgactataa agcaaaataa tttgatgagt aaccataata agattgcaga
    16201 ggtataagac aatatttaag aagtatagaa aattaattta agaagtataa taagctaatt
    16261 taaagaagta taatgagcta acttaagaaa tatagaaaat taatttaaag aaatataata
    16321 aattaattta ggaaatatag aaaattaatc taaagaagta taataaacta atttaagaaa
    16381 catagaaaat taatttaaag aaatacaata aattaacttt taagtaacat taaattaatg
    16441 taagtaaaat aacgttcatg tgaagattta aaggtgcatt ttctggagct aagtatatgt
    16501 tgtagagtgt atatctctct agtctgtgaa gtttaatagc tcaagaaaat gcactattta
    16561 tgatgcttat tttgttttat ttccaatttt agaaataact attctaatta aatctaaaat
    16621 tataccataa ataagaaaag aaagtataga tgctatgtac caataaatcg aaattgaccc
    16681 tgtaattgtt ctacatggga tttgcatacc tgaatccata aaaataaaaa aactatcaat
    16741 tctggaaata atagacaaaa taggattgaa acctattaga ataatacttt ttgagtcttg
    16801 accaatttga tgcatagaaa tctcaaacaa tgcaattaaa aaaaaccaaa atgtaaaaga
    16861 tttaataatt tttttcatca aaatacctca cctaatataa attctgtttg cactatttgc
    16921 ttactataac tatttatctc cattcagtta catcatttaa ttgtaatcta ggtcttcttg
    16981 atggttcttc atcaggatac cccaaagata ttgctgccac taattgacta tctgtattca
    17041 accattcaca taattcacga tatgcaaagt atacatcaca aatccaaaga ctacctagac
    17101 ctaattccac ggctttaagc gacatatttt gaattgaagc tccaattgat tgaatgtttg
    17161 ccatttcata gaatcgttct tcaggggata gattttctaa aagtgactta cctagtatat
    17221 ttaaaataaa aattgttact ggagcttgtt tcatgatttc aaccgtgtaa tttgcaccag
    17281 agatatgttg acgacttcct ggaagaagcc cattatcatc aatttcgttt tgaatacctt
    17341 tgctcatggc tttaagcata gattcttttt ccttttcagt gatgacaaca aaacgccatg
    17401 gttgcctgtt tttagatgaa ggtgcattta ttccagcttc aattattttc tctatagttt
    17461 catgtgaaat gctttgattt ttatattttc tgatacttcg tctttttgat atagaatcac
    17521 ttatcatttt aagtaacctc cgtttatttt ttggaaaaat atatttgttt tctattttta
    17581 attattttag cattaatcag aaaaaaagtc attgatttta gtatacaatt attaatgatt
    17641 gtattaagaa ttgcatcaaa aagtatgtga ttatactaag ggattatatt agacattata
    17701 tttaaaattg tattaaaggt tgtatttaat attatattaa gatttatatt atattgggtc
    17761 ttatattaaa ttaaggatta taaattgaaa ataagctata aaaactcaat attgttttgt
    17821 tcagagataa aaaaagaatg ccttaggtga tttttattaa aaattaagag tgataagatt
    17881 atatacttta taaatgtaaa tatgacgaat aaaaatgata aatataataa tctatatatt
    17941 tacattagga gttttctata ctataattat ttaaaactaa atataaatgt tggaattatt
    18001 taggacttga aattgtttaa aaataaatat aagtgatgaa gaggtgatgt gatgagatta
    18061 gatatggata gtatgttata tgtactaatt tatatgacat tattggtatc tgtcattgga
    18121 agtatatttt atttttgtaa agacctctta aatcataaaa ttaagtatgt tttaataaaa
    18181 aacatatttg taaatagaac tacatattct gaagaaaggt tgcctaaaca gtatatagtt
    18241 tttcagaaat atgatatttg gaggtattgt agtttgtttt agtttgaaat gaatctatta
    18301 taaattctaa agtttaaaaa taaaagggag agaataaaat ggatttcatt tcaagtatat
    18361 tgcaaggatt attaagtata ttatttaatt ttacaggtga ttttgggatt tctatagtac
    18421 ttataacttt aatagttaaa ttagtactta tgccactatc attaaaacaa agattttctg
    18481 taaaaaaaca gcaagaatta gctgaaaaaa tggaacatat aaaagaaaag tataagaatg
    18541 atactaaaga actggaaaag caaatacaag taaattctgt agaaagtatg aagagtatgc
    18601 tgggatgtct tatagtttta gtacaaatgc cagtagtata tgctttatat catgtatgtt
    18661 tagatatgac aaaggaattt acaacaatta taattccatg gattgctaat ttaggtacat
    18721 ttgacaattt gtttatatta ccttgtatat atacgttaat tatgttagct tcaaatttag
    18781 tgaattatat accgtactta aaaataaatt ctcaggtaaa gtttaacaaa caaatggcta
    18841 tggtgactac tataacaagt cttatactga ctgttagaac tccagtagcc ataggtctgt
    18901 actttataac aagtgcaatt tattcattaa ttgaagatat ttgttttaga atatatttta
    18961 atcgaaaaaa tagaattgtg acaaaataat ataattatta ttagaaatat tttagcctgc
    19021 taacatagga aaagttagta ggctattttt atctatgata tcaattccat taagaatagt
    19081 ctaaaaaata ctatcaaaat actatccaaa tactataaaa aaatagttga aaaataaata
    19141 aattaatata aactaaaata aagcctacaa aaaagaggtg ataaatagaa atggctaaaa
    19201 tactagttgt agaagatgaa aaaaggatgc aaagtattat tgtagaatat atgcaaaagg
    19261 gaggatatac ttgtgtaact gctgatgatg gagtcgaggc acttacaata ttaaagagca
    19321 acaatataga tttaatgata ttggatgtta tgatgccgta tttagatgga ttttcagtgt
    19381 gtagagtttc acgtgaaatg actaatatac ccattattat attgacagct aaaggtgaag
    19441 aggaggacaa attaaagggt tatgaatatg gtgcagatga ttatataaca aaaccattta
    19501 gcccaaaagt acttttagca aaagttaatg ccttgttacg tagatatact actggcattc
    19561 caaaaaattc attgatttta ggaaagattt ttattatggt agcatcaagg caagtatatg
    19621 tagaagataa attgattgat ttaacatata aagaatttga attattgcgt ttatttatgg
    19681 agaatccaaa tcaagttttt tccagagaga aacttttaaa ttgtatatgg ggatatgatt
    19741 ttgaaggaaa cacacgaact gtagatacac atataaagac cttacgcaaa aagcttggta
    19801 gtgaagggca tcatatagtt acattaattc gttcaggata taagtttgag gttaaggaat
    19861 gagtagttta agaaagatta tagaaaaatt gaaaaattta gatatttcaa attggattaa
    19921 acaaaatttt ggactacatt cagttagaaa aaaagtgttt ttactttcta aattatcagg
    19981 ggcgttaatc atactatttt atctggttgt tgaagaactt tcgataaact ctaaatttga
    20041 attttggatt tggtttataa tactggttat atttattatg agtattgatt ttttattggg
    20101 aaaatttatt tcagaaccaa tcacatccat aaataaatct gcaaaaagca tgtctcaact
    20161 tgatttttct aatccatgta ctgtaaatac taatgatgaa tttggagaac tttctaggag
    20221 cttgaataca atgtctacaa atctacaaca ggcattatca gacctagaaa gtgcaaatat
    20281 tcaacttgaa aaggatgtaa ataaggaaag aatgctattg gaacaaagaa aggagttggt
    20341 agatacaatt tcacatgaaa tgaagactcc acttggcatt atacgtgcgt atacagaggg
    20401 gcttatggat gaagctgatg aagaaaaaaa gaaaaattat atgaatgtta taatagaaga
    20461 aactgataga atgaacaata tgattgtatc attattagac ttatctgcat tggaagcagg
    20521 agtttctaaa cttaacccag agcgttttga ttttatagag tttgttgaaa ctgtggcagg
    20581 tcgtctttta attgatatac cagatgccaa cttttatttt acatatgatt tgccagagta
    20641 tgaagttttt gtagtggtag ataaaatgag aatggaacaa gtagtagaga atctaattat
    20701 taatgcaaag aagcatgttg tatataatgg taatttagat ttatcgatta catgtgaaaa
    20761 tggcttgatt ttatttgaaa tttataatga tggtaggatt atagagcaag atgagatttc
    20821 aaagatttgg tctaagtttt atcgtagtgt tcaatctcag cgtacaggag gttcaggtct
    20881 agggcttgca attgtttcac aaatacttac aatgcaaggg ttagaatatg gagtgaagaa
    20941 tcgtgataga ggagtagagt tttattttat gattccaatt gatgaataat ttagtattaa
    21001 tttataaatt ataagtattt gatgtaaaat atttttcttt gtagacaata gatatctaaa
    21061 gagaataaaa taaaaaaatt taatgaaaaa agcttaatga aaaagtctaa taaaaaaatt
    21121 taataaaaaa atttaataaa aagcttaatg agaagtcttg ttttcctgat gaaaacaaga
    21181 ttttttatta agctttttat tatttcacct tgatttcaca agagagacac tttgatttca
    21241 ccaaatagaa ataaaataag ttaaataaat taaaaaatag aaacgtaatt aaataagctt
    21301 acaaggggga gtacttatga aatggtatac atggttgact attatattga tagtattggt
    21361 ttctattccg cttaaaatta aatttataaa atggtgggac agacgacaaa aaaaatgata
    21421 aaggaggtta tgatagatga taaaagtaga agagttgaaa ttttcttatg gaaaagataa
    21481 acagatatta catggactta attttgaagt aaaagaagga gaaatatttg gatttcttgg
    21541 acctaatggt tcaggaaaat ccacaacaca aaaaatctta aatggtgttt taaaaggtta
    21601 caatggtcac gtatccattt ttggtaatga agttaaagca tacacagaat ctttatatca
    21661 aaagattggt gttctatttg aatttcctta tttatatacc aatttaagtg caattgataa
    21721 tttagaatat ttttcgtctt tttatcctaa aaatcaaagg agagacatat atgaactctt
    21781 agatttgtta gagtttaaga aagagtttat aaataagcct gtatcatcct attcaaaagg
    21841 tatgaagcaa cgtattagca tggcaagagc attggtaagt aatccaaagt tgttgttttt
    21901 agatgagcct actagtggac ttgacccatc tggagctgta ctatttcgta agattattga
    21961 agaagaacga aaaaaaggaa caactatctt tttgactact cataatatgc tagatgcaga
    22021 tttaatgtgt aacaatgtgg cttttattgc agatggcaag attatggcaa tagataaacc
    22081 caaaaattta aaaatgaaaa atagcaataa taaagttgaa gtggaatttg tatataatgg
    22141 aagtcgagat attaaaattg tagatatagg agaattagaa tctggaataa cttttaaata
    22201 tgacgaaatc ttaagtgtac attcaaaaga agctacacta gaagaagttt ttattaaata
    22261 tacaggaagg atgttagtgt aatgtattct aaatttttta ccttagtaaa aaaggatttt
    22321 tggatgatga tttatggtaa attttttgta ctaacacttg gctctcttat actttattca
    22381 tgttatatta attttggtta tgtaaagttt atggatgaag atagttatca caataatgta
    22441 tatctatata atccagagaa tataagcatg agtacatctc cattaattaa tcaagtttct
    22501 tctataggtg aattaaaagc aaaacttgat gaagatacaa gtgcagtttg tattgatatg
    22561 agctccagta agccgaaagt aattttacat gagagtatta aaggcgttga caattataga
    22621 gctgactatg cattgtcatt attatcatca gaaaaagacc ataatagcaa aatatcggac
    22681 aacaatgcaa taattcaaat taatgataag gatacatctg agataaaaca aagaaaagaa
    22741 atgacttgtg agattttatt ctttgaaatt gtagctattg gatttttagg gattgcctct
    22801 ttattattta aagaaaagca gatgggggta atgcgtatta ctggtatttt acctgtaaaa
    22861 agaagccttt ttatattatc aaagatattt gcttttttga tatgtgacct atgttttaca
    22921 gtgcttttag tagtatttaa tgttggattt tttgaaggaa taaaaatact ttcacaagta
    22981 ttagttcaag ctagcatttt atcaattatt atgtctttga ttggatttgg atgttcaatg
    23041 ttattgagag attttaagca attttctctt atatatcttg tggttgctgt atttataact
    23101 acacctatat ttctatctgc aaatacatct tttgaaatag attggattaa atatcatcct
    23161 ttctaccatg tttatatcca gcttaaaaat gcgtttttca tgaatttttc aaatgaatta
    23221 atctattttg tagtgtgttc cttgacaata atagttctat ttttacttgt aaaaaatatt
    23281 tttaataaag agttggttag ggagggataa tcttgatagg attacgttat cagttgaaga
    23341 gtataagaaa agataaaatg tgtataattt cattcttttt accagttgtc atggcgattt
    23401 taataaattt tatgggaaca attgatatgt cttcattagg agagatacat tttggtatag
    23461 taaaggatac acttcctgta cgaacagagc aatggttgaa gaagtatggt gtgttaagtg
    23521 tatatgacac aaaaatgcat ctaatacgtg aaattaagga tattaaaacg gatacaattg
    23581 gagttgttag agatggtaaa gggataaaaa ctgtgcttta tggaaatgag attaatgcta
    23641 taaaaaatgt tggaaagaca ttgccagaga tgtttagact aaagggagat ttaaaagatg
    23701 ttgaggttac aatattgcct aaagatagta ttttaaaaga gtttcaaaat gtttttattg
    23761 ctatgatttt aattactgct atgtttatgg gatgcacctt taatgcaatg aatattatat
    23821 ctgaaaaaga agatggaata tcatttataa atgaaatttt accacagagt agaagtcagt
    23881 atattataca aaaagtattt ttgggtcttt tatgtgggtg tctatcttca atgataactg
    23941 tatttatatg tttggatatt tcaatattag atatgataat gatgttacta ctcgttgtat
    24001 tttcagcatt tatttcatca cttataggtt tatttattgg aaagttgtca gaaggtctta
    24061 tggtaggaat tgtttatatt aaaatagtaa tgattttatt tataggtgtt cctttgttaa
    24121 tatatcttct aggggttaat acaagcggta tagggaattt atgctatata atgccgtcag
    24181 ttgctacatt taatggtatt atggagatta ttaatggtaa tgtcattatt ataaaagata
    24241 ttgtaatttt ggctgtacat tgtgtagtat ggtttatgct gtatattatt ttagataaaa
    24301 aaagaaagtt atcttttagt agataactca atataattat aattagaaca atttaatagg
    24361 tgatttaata tactttgtat agtgtaattt atgtagtaca atttgtatac tttaatttat
    24421 gtagtgtaat agttagtttt attttataat tattcaatat aatttagtaa gaagttcagt
    24481 acaattagag ctacttagtg gaaaatttaa taggtactta aactatataa atactttatt
    24541 ttcctccatt tgtactataa atttaattga tgatataaat ggaggtatta ttatatgatt
    24601 tgataaattt catcattata acttacaagt aaatgtatta ttatatgaga tattattata
    24661 taaagttaat aaagatagta tcaaaatatt atttgttata aagagagggg ctcaaaataa
    24721 agataaacct atttttcaat gttgaaatga taaaaacata tttgatttta tataaaaaat
    24781 tttcttcatg aattaaaaag tccattttga gacccctcat ttttaaccag ataaatcgag
    24841 ttaaactatt taaatatatt actcggattc atttgactta gatattcctt gcttgtaaaa
    24901 ttgttttgag tagggaatag ccaaagcaaa aaataaaagt ataatccctg ctacgattat
    24961 taaattatta atagacatta catctgccat tgggccgaaa atcatcatac caagaggaat
    25021 aaaaccagta tatataataa taggtaagct aagaactcga cctagcattt gtggtgctac
    25081 atgctcttgc aacatagtca tagctgatgc ttgaatgatt ggaattgaaa aattcgtaaa
    25141 aaacataaat ataaaaaatt gccacgcttg tgttacaaga cctagaataa ttgaaaaaat
    25201 agcataagat aatattgcta gaaaaaaggt cttatttcta tttttaaaac caccaaaaac
    25261 tcctataatc aatccaccta aaaccatacc taaaaatcca atcatttcaa taatagacag
    25321 gtatatataa cccttgccaa atgttcgttc aataagaagt cctataagaa atcctgatgg
    25381 aacacaaaga agcatgaatg ctccataagt gaaaagcact tttctaatga acttatctga
    25441 aaaacagtac tttatccctg ataccatatc tgcaaaaaat gagacttttt cagtttgttt
    25501 tggtatatca tgtttaggaa ttttgataaa tatcaataat acaatagcaa taattgcagt
    25561 aaatacatca attaaaagaa cttgatttat tttattacca aatccaagga ttgcagcagc
    25621 cacaatagga actataaatt gtactataga ctgtaggcta ccatttatac cattaaaacg
    25681 cattaaatgt tcttctggta caagttgagg aatcattgcg tttacagcag gtgtttgtac
    25741 accacttcct agtgaacgta taacagatat tactgatatg gctattaatt cacttgttgt
    25801 tccatttgat gacatcataa atagaattag aactaatgta gatatagcaa ttgttgaatc
    25861 tgatacaatt atgagtacct ttcgattgta gtagtctgcc catacaccag caaaaaaaga
    25921 aattatcatt tgaggtataa aggagcatat agttaaaact gttacccata cacctgatga
    25981 tgtctgaagt gtgacatacc atatcattgc catctgtaca agtgatgagc caaataagga
    26041 aatatattga cttgctagaa aaagaatgac tcttttcttc catgtagaat taatattttt
    26101 gttttccatc ctattcctcc tttgatttgt gtgtttaatt tttctcgctc ttaaggataa
    26161 cacacaaatc aaagtctgga gttagagtgg agggctagga aaattgtttt ttcataattt
    26221 gaagatgaga tttcattgtt tttgcattat gttcagcttc tttcaatgaa gttatcagtt
    26281 tgtcacacac tgagataatt tcgtcatcat cttttggagt gtcaaggaca gttttaatat
    26341 tggtattagg attagaagat aaagcattga gcattcgtaa aatagctgaa agagaatagt
    26401 ttgcacaacg aagtgcactt ataattttaa gtcgttttat atcctcatca gtatatacac
    26461 gataaccatt ttgttttctt tttactttta acaaaccatt catttcccaa tttcttaaag
    26521 tatctattgt aacatttaag tgttctgcag tttcttttct agttaaaaag agtttatctg
    26581 tttctggagt atgtccattt aaaatctgtg taacaatagt aagtgcttcc tcggcattag
    26641 atttttctat ttctaattgt ttcaaatatt gatttacaag acaaatagcc ttgtcaaaat
    26701 ctctatttgc tgatgttctt acaatctcaa gggcatcttt tcttaatcct ctttgtacca
    26761 cttcaatttt caaggctgta cgagcaaatc taaattgttc tatatgaaaa tcattaaaga
    26821 tacgataacc attggtatta cgcttaggtt taggtattaa gcttagctct tcatataaac
    26881 gtactgtatt aggatgtaca ccaattaaat ctgctacctc tgatgtcgtg taagtgtcca
    26941 ttagtattca actcctttta gatatatagt attagtcata ataacatcaa acaaaagtct
    27001 ggtgttatag tggagggttt ggttatctaa gagtaattag tctatttaag attaattact
    27061 ctattataga agtaagtata tacttttcaa taaactccca tctatcacga ggtttaaaca
    27121 ctattttaga tggtatggct attactaagt catattgtgg aatacaacaa atagcatttc
    27181 ctccagctcc cattgctgaa taccacaaaa ctccatcttt tttacatagc caccacagat
    27241 aaccgtattc attactattc attttaactg attcttcaat ccacgattta gatataatac
    27301 gtttattatt ccaaatacca tcatttaaat acagaaaacc aaatcgcaac atatctctag
    27361 cactcaatgt aagtccccag ccacctgtag aatagccctg tctatctttt gcccaaccct
    27421 taagattttt tccaaagaaa tcttcaaaac caaaatcttt catatcataa tctggtataa
    27481 ttttcatacc aataggttta aataagtatt cattagcaaa ttcacgagca gattttcccg
    27541 tagtttttgt tataatagct gaaagtaggt gtgttccagc tgaagaatat ttgaaagtac
    27601 caatttcacc tttttgacca agctgattca aggtatattt tatccaatct ttttgtttgc
    27661 aaagtctctc taaagactca gtcatatttt taaaagggta tggtgcagtc attgtaagta
    27721 ggtgacgtat agttactgct tttttacata tttcagaagg actacaagta tattcaggga
    27781 aaaaatcaac aaccttttgg tcaacatctt ttatatatcc tttatctatt gcaatgccta
    27841 ttaaagctga tataatactt tttgtaactg atgcaacatg aaaagtatcg tcagcattat
    27901 atccattgta gtatttttca aagacaactt ttccttttct tgcaataatt attccattta
    27961 tattgctata ttgtgagatt attaaaggtt caagttgtga aagcttttct gaactcatac
    28021 caacacttga agggtctact ttttcaaaaa ttaagtctaa gcaattgttt ttattcatta
    28081 aagatacctt cctgttatag attttggatt ttcttttaga gtttttggag aaccttctgc
    28141 aattatataa cctcctttgt cgccaccttc tggaccaagt tcaacaatcc agtcacaaac
    28201 atttaataca tctatatcat gttcaacaac aattacagaa tttccatttg ctactagttc
    28261 atccaacaat tgaatcagct tagcagtatc atatagactt agaccagtag ttggttcatc
    28321 taatacataa agaatattac catttcgttt tttaccaatt tcttttgcaa gcttgactct
    28381 ttgggattcc ccaccactta aagttggtgt aggttgacct aattttatat atcccatacc
    28441 tatttgttct aacacattta agtgtgaaac aatatttgga atatctttga aaaaatttac
    28501 agcctcagag atactcatat ttaaaatatc atgaatagtt ttatttttat atttaataga
    28561 taaggattca tcattaaatc tttttccatg acagtcactg catatatggt ctatacttag
    28621 attatttcct aaccagactt tttcatatcc gctaccacta catttaggac aagccccttt
    28681 ggagttaaat gagaaatgac cagctgtaaa tcctcgctct aacgattctg gctgacctgc
    28741 aaatagtgta cgtatcttat cccatatacc aatataagaa gcaggagttg agttcatatt
    28801 ttttccaata ggagattgag atatttctga atatccagaa atatacttag taccaatcaa
    28861 tctatctgca atagtttcga caactgcata atcatcttca tcacctattt cactctctga
    28921 agaatttgtt tgattattga tagtatggtt attaaattga cttctaagaa gaggaagtaa
    28981 ggtgtctgat ataagtgaac tctttccact acctgatttg ccagatattc caaccatagc
    29041 acccaaaggt aatgatactg ttacattttt aaggttattt gtatttgcat tttgaacagt
    29101 tatacagggt atttcatcaa tatgtgtaat atttttagtg cttgttctat taggcattga
    29161 atattttcca gatagatatt gtccagtaag tgatttattg gattgaagta gaccttccaa
    29221 atttccttga tatactaatt gcccaccttc aataccagct tttggcccaa tatcaataat
    29281 atgttctgcc atctcaattg tgtttttatc atgctcaact actataactg tattgcctaa
    29341 atctttaagt ttctttatgg attttaagat gtctattttt tctgactcat gcaatccagc
    29401 agttggttca tcaagcacat aaattaatga atccatctct gaatcaaggt gtgaatttaa
    29461 aaatagtctt tgcatttccc caccactcaa agttgatatt tctctgtata aggatagatg
    29521 acctaatcgt gattctatta gatgtcgagt tttggacaaa atattattta caaggtttgt
    29581 accaaatgtg cttaaattac cttggtctaa taagatttta aggaactcat caatttctag
    29641 aatcgtcatt tttccaagtt ctccaatatg ttttccgttt aaaagtactc tacgtgcttc
    29701 ttcaccaatt ttaaaaccat tgcaatctga acaggcagtc atagtataaa ctccatttaa
    29761 atcttcgcct cttttgtaac gattgtgaaa tacctttgat aaagaggaac tttttttgcc
    29821 attttcataa tgaccataga gcacttcatt ttttacttct tcaggtaatt gtaagaattg
    29881 tatgtctaaa taatcgttga attttttaga gaatactctc aaatatcctg gtgtaatctt
    29941 tgctctatct aagacttgtt ttagggtatc atgcttatca aggactaatt tttcaatatt
    30001 aatctcaaaa tatgcaccat gcccagaaca tttcatacac attccatctg aagagttgta
    30061 tgagaaataa cttggcttta atcgttcttc ttcataccca caattattgc aaattaaatt
    30121 atcgcttaca ggagtatcac acatagtaca cattatttga ccttctactg aatacaatag
    30181 tgtaagcatg ttgagaatat tggttcttgt gcctactgtt gaacgaggat tactttggcg
    30241 aataatattt tgttgaacag ctatagtggg agctaaacct tgaatattat cgaacttatc
    30301 ctcactatca atacctgcaa gtatacctaa agattgaagg tattgtttac gtccttcctc
    30361 aaatacaatg tcaaacataa ggctagattt acctgagcca ctgacacctg ttgcaacaac
    30421 cagcttatct ttgggaatag aaacatctat atttttaaga ttgtgtatct ttgctccatt
    30481 aatttttatt tctttcattg gttcacctca ttttatcaat tttatcaaac cataaaatag
    30541 ggatatatta atccttaata caagaataga ttaggtgagg ttattaaaaa ggttctatag
    30601 gtaaatatat atcgaccaag tgtctatttt gagggtgatt gtgtgggttg ttaagatata
    30661 cttcaaaagg gaaggaagca cgtagcttat aattaacatt tgataaccat tcattataca
    30721 taaaatccca cgcatcacta tattcagatt ttgaaatatc aaaatgtcct acaagatatt
    30781 ttccagaagg tattatcata ttgccaagat tgtcattctc tttgacaaca atgttatctg
    30841 gaatggtcat gcaaatacta gtccttaatt gatgttcctg tgtaaattgt ggattatcat
    30901 gataaattgc taatatttgt gtgttttcaa gtccaagtag attctgctca cttgcatatg
    30961 tgaataacct ttctaaaagt tttgggaaag tactagttag attaccatat gaaccagtat
    31021 atctagtgta aatagtattc atattatcaa gttctagaat ttcaatttca cctttaacct
    31081 ctctagtatt cttacattta ggttttgata tgcttctatt gtactgagaa attttatatg
    31141 gttctttgca attcttgcta taattatttc tatattttat aggacttatt ttatagtaat
    31201 ttttaaatgc tctcgagaaa actgctgaat cagtaaagcc gaagtagtgt gcaatatcag
    31261 taatggtcat gtcagttcga tgaataagaa aagcagttgc attctctagt tttagcctat
    31321 ttacatactg tgccaaagtt tctttttcta ttgctttgaa gattctatga aaatgaaact
    31381 ttgaaaaacc tgctatatta gaaagttcgt caaggaaaag gggttcatgt aaatgttctt
    31441 ctatatagtc ttgcactttg caaatacgat ttagatattc ttttttactt ttagcgtttg
    31501 tcataaatag ccctccttat tgtgtaaaat gtgtattaat ttatagtaat atagtaataa
    31561 aattttatgt ttttgtaatt tatgataaat aagatagcgt tcaatttttt ataattaata
    31621 aaagctattt ataattaata aaaaatgtaa gatgcattat atctgtcttt gcatataagt
    31681 ataatcttcg taataattag tataccatag tgatttattt ttaattgtgc atttatataa
    31741 atacacaact tatgatatgg aagagttatt atgcaaataa gctgtaatta aataaattac
    31801 aatataaact agttaaaaca taagccagaa aaatactatc aaatttgtta cttagcacaa
    31861 gagcatagag gagataaaat aagattgaaa aattacatac taaatttaat aaaattttta
    31921 tattaaaaat aatattaagt ttattgttta aataaaaaag ttaaatctac actattaact
    31981 tattatactt aaaactttaa aatgaaaata tcaaagaata ctttttaata aaaataataa
    32041 tttaataagt tatttggaaa tctaaaaact ttacaacaaa ttatctattt aaattggagg
    32101 agtgggaata tgagtaatag tttttatcaa ttaaattcag cagtattata ttttaatgaa
    32161 aaaagagatg taaatagaat aaggtttaat cactatgtaa tgagaaaaga aaatatacaa
    32221 agattaaaag gaactctgcc aagaggacaa tttatgatga cagtaaggaa agctgcatct
    32281 gaccttgatg tatctacatc tacaataagt agacttgtaa atgaatttat agatttaggt
    32341 atattgagat taataagtag aggagtaaag ggaaattgtt gttctgtata tagttatgtt
    32401 tgttctgaaa atagtaatac ggagatttta acagggaata gtggtatttt caaaaacata
    32461 gagaaatacc ttgatgtaac cattaacaat aatcaaaaaa caatgaaata taaacataga
    32521 gatgagttgt tcaagaatag taataccaac acatacagcc gtataaatga agaacatata
    32581 gataaaggtt atgattcgat aattcatact gggtatgaaa cagaaaacga gacaaagaaa
    32641 aaagaattat taaaaaataa tttaaataaa aagaatttaa ataaaaatac ctttgaaaat
    32701 tcggatgaag atttgaatta tgaaagtcca gaaagtatac atgaaattat tataagaaaa
    32761 ttaaatagtg aatcgggaaa aaattttaaa gctgatttaa gtataactaa ggcacttata
    32821 gataaaagat taaaagaagg gtatagttta gaagattttt acaaggttat agaggcaaag
    32881 gtaagaagat ggagatatac agatatggaa atatatatag aaccaacaat tctgtttggg
    32941 tatgaatttg agtcgtattt aagtgaggag agttctttat ctgcttgtaa ttatggtgat
    33001 atgtgttatg aggatatgta ttgagatatt tattaagata ttagttgctt tttagttgtt
    33061 taaaatatgc ttatatataa gtataactag tgtattaaaa atggaattgt ctcaaaatag
    33121 ggagaagcct ctttttaatt gtttaaatga tgaaaatata tttgatttaa tacaaaggag
    33181 agtgcctcat gagctagaaa gctcattttg agacacccac tttttttaat atactggaga
    33241 aatataaccc atttgaatgg cttttgtaat agcctcaact gatgatgaaa catctagctt
    33301 gtcatatatg tgttgtaaat gatttgataa tgttctttca cttatataca attcatcagc
    33361 aattattttt ctttttttac cactacagat aagctgtaaa attcttttct ccatactagt
    33421 taaatcttca ataattactt cagtattttc agatggaaaa caagttcccc cttgatagac
    33481 ttttaataat gaatcaatta aactatcagg gctaatattc ttatttagga atccatagat
    33541 acctattttt ttagcctcat gtttataaac tgggaggtcg tatccagtca agattattat
    33601 tttaatagtt gaattagttt ctaataaatt ataagctact tttaagccat cacattcact
    33661 taagcctcta aggtttatat ccattaagac aatgtctatt tttttatctt gaatcatttc
    33721 tgatagacta tggatgtcct gtgtggaata aaactgttcg atttcttcgt attcttctag
    33781 tgcaatggac aagcttttag aaaaaagcat atggtcatca actaacaaaa tatttataag
    33841 aaacatctcc tttcataggg attgtaatct gtacacaaac tccatgaggt atattatcag
    33901 aaatacttat cgttcctcct agattttcta cttgttcttt aattgagata atccctttgt
    33961 gttttattgt atcaaaagta attttagtta tatctaaagg cgttttaaaa gatgtatttt
    34021 tcacaccatt atcattaata tttaatttaa ttatattttt ttctagtgct aaagctatcc
    34081 atgcatggct accatttgaa tgtttatata cgtttgtcaa taattcttta attagtcgat
    34141 atatcaatat atcatatggt tcaactaaga ataaagtatc agaacactta aatgaaacac
    34201 aaatgttttt ttgtggaaag gatgaagaaa cactttcaat taaattctga taattttctt
    34261 ttactgttaa attttttaaa ataacagggt gatagtcttg catttgtttt cttatacatg
    34321 tatttagatt gttaagtgtt tcatatataa tatcttgaac ttctgggcga tatgacttac
    34381 tcatcatgtt ttttattgaa agcaggtttt gtaatatatc atcatgtaaa aaattagaaa
    34441 actctgtttt tagttgttct tcttgttgta atttttccaa tgcaaaagta tacttgctat
    34501 ttttaactac tttatttttt tcatctctaa gattaaactc caaaataatg ttacagagga
    34561 aaataagcat aaataaaaca tttatagcta ttaatattat agaaaaattc ccactggcaa
    34621 atagtacaac caagctaatt actaatatac ttataaaaag aaatatagac ctttgcttta
    34681 tgttaaaaac tgttgaaata ggaaaactat cattttcttt aagtgcaata ccatgaatac
    34741 tcataaaaaa tactaataca gttagataga ttccaaaatt accaatatga tttgatattc
    34801 ttagtgtaaa aaaataataa gtaaggaatc ctataattgt tataaatacc gaaatcgata
    34861 taggtttttt ttcacttttt atgacaaaag cgaccctttt ccaatgataa aaaataatat
    34921 agaaaaagct tattatacta acaacaaact gtattccgta catacaagca tacgttctgt
    34981 tagaaataaa tgctcctaca attgcaccaa tgcaggtgat aagcaataaa atgtctgtta
    35041 tcttacggaa cttgtatcca ctaccttgaa acaaaaataa aaaaataaat ttaatagaga
    35101 catacgctat aactgtgctt aatattttaa aaataatagt gcctattggt gtggctacaa
    35161 atgataagag taaataccaa ctgtctagta ctagtaatcc acaaaacaat gaaataatgt
    35221 tattttctcg gacattacaa gaggttatat atgtgatgtc tatgagtaat aatgttgaaa
    35281 ttattaaggg aataatgctt tggatattca tataagtttt agtgcttgta gatattccca
    35341 tgaagagaag tgatagaatg tagtttccta aaaatagtat ttttaaatta aaatttgtca
    35401 atcttataca cctccttatg agttaattat attaatttta tgactaaatc aaacttaaga
    35461 attaataact ttgaattata ttaattttat gactgtatca aatttaagaa ttagtaactt
    35521 tgaattatat caattttata aatatatcaa acctaagaat ctatagtttt aaattatatc
    35581 aattttatga ttgaatcaaa cttaggaatc tatagtttta aattatatca attttataaa
    35641 tatatcaaac ttaagaattt ataattttaa attatatcaa atacttataa atgaagtata
    35701 ccaaatcgtc acacctttaa gcttgacgat ttggtaaata ttattttttt agtacaattt
    35761 tacaacagga tttaatagta aatatataat atatcaaata cactacagaa gttaagaaaa
    35821 ttgcaataat cacaggtatg tattgtgaaa gtgaatttcc aagtaaattc tgcatcaaag
    35881 cagttttata aaccaaggta gcaaatatac aatccactat accaagtaaa aacggaatat
    35941 taaaaaatgg caatacttgt tttttaataa tttttttaat ttgtatatta gtataaccca
    36001 ttttctgtaa aattttataa tcagatgttg aatctgaaat tgctgatata ttgttaaagt
    36061 ataaaatact tccagaagca ataaagaata atactacaag aaatccgatt aatagaaatg
    36121 tagaactatt aattgataat aaatcattta tcctgtgaga atatccttgt aaataagggc
    36181 ttccatctaa ataatctgaa atattactat ataaattttc attattttta attgatttac
    36241 cattaatact taaaatgctt gtcattggga atttactttg tgctatttgc tcatatatac
    36301 tatcattaac gataagtgtt ccaacgctat tagcaaaaga aatagcattg tttaatgatg
    36361 tttcccttac agttacaggt gtttctttac cattaataag taatagataa atcgttccat
    36421 tttcactata tccatcattg tttggttgat atttcattaa aatacattca ttatccctta
    36481 aaggggtaag ttgttttatt agctttgttc gattttgtgc ctttaacaaa gtataataat
    36541 cagaaaaaga tatacattca aatcctgatt ctcttgaaat aacttcttta gaagaactac
    36601 tattagataa attattctta gatgcaccaa tttcatattc aaatggaagg tcttttgatg
    36661 aagattttac cttataaata tttgtctgtg taaaaataat atcgtctgta ttagacatgt
    36721 attgactgac aatttgtttt acatcttcta tttgtttatt tttttctact ctaaattcta
    36781 tctctgaagg tgcaatacgt gaaacggatg caatagggta gtataaagac agtgccatga
    36841 cacttgaaac tgtcaaagta gcagcagaga gtaatgtaag cataattaga gttttagagt
    36901 ttgaacggat acgatagata aagtttggaa tagttattat ctttgtacct gtatagaaag
    36961 ctcgtttatt ttttttactc ttttgcataa tatatggaag aaaggaattg ataaagagaa
    37021 ctgtaccaat tacaatgata atcatagtta tcaagcctat ttgataaaat ccaatactta
    37081 tccatactga cttagctcca cgaagaatat ctagtgataa acaataccca cttaaaatca
    37141 taataaaccc cataaaggct ggaatttttc tgaacttcaa ttttttttct gtgctttttt
    37201 catatcgtat caattttata agtgaagtct tataaagaaa tttaccattt gatatagcta
    37261 agactaaaac aactaatgct ataaagataa ttgttttgtt tattgcattt atattaaaaa
    37321 atggtatctg tgaattatca attgataaat taagtagtgc tgtaattcca aagacaatac
    37381 tcttatgtac caatgctcct aataccaatc caataataaa agctccacta caagttaata
    37441 tattttcaaa ggtaagtagc gataagattg taaacttacg atatccaagt aaagcatata
    37501 tgcctaattc ctttgtacga cgccttaaga agaatcgatt tgagtatgcc atatagaaga
    37561 tgacaaatgc catcaaaaat atcgaaataa cattacacat agattcaact ctaccatcag
    37621 ttgaaatttt ttctagcatt accttattaa cagaaaatga agtaaatgcg aaaaatacag
    37681 taataacaaa agagactgaa agtagatata gagaatagaa tgaaaagttt tttttaagat
    37741 tctttattgc catataaaaa atgttcatta aattatcctc ctatctttta gtatccaatt
    37801 ttgcaatttc ttgaataatc aactcataaa atgtatgtct attattctgt tgtttattta
    37861 attctttaat gatatgacca tctttaaaaa ttaaaattct atttgcaaaa cttgctgcat
    37921 aggcatcatg tgttaccatt aaaattgtcg ttttcaactc tttatttgct tcttgtagtg
    37981 agtggagtag ttcagtagct gaattagaat ccaaagctcc tgttggttca tctgcaaaga
    38041 gaatactagg ctgttttact attgctctaa ctgctgaagt tctctgtctt tgcccaccag
    38101 ataattcatt tgggtatttg tttaattgag aagctatgcc aaatcgttca gctagactct
    38161 ttaccatttt ttctatttta tctggactta gtttttgaag tgtaagaggt actgaaatat
    38221 tttcgaaagt tgtaagacta tctagtaaaa aatactcttg aaagataaag cctaaattat
    38281 ccttacgaaa atcagcagca ttagtgtcat taagcgattt caaatttata ttatttatga
    38341 aaatgtctcc acttgttggt ttatcaattg tagataatac attaagaaga gttgttttcc
    38401 cagaaccaga agctcccatt atacctataa actcaccttc tgatacatta aaggatacct
    38461 tatctaaact cggctgttgt cctttataag ttttagttat gctatttact tttaatattg
    38521 tattcattgt atatgcccct ttcatcttga tatagtcaaa ttatatatta accttattat
    38581 tcaatcatga gtaaatcacg caataattct acacaaatta cttatatcat aatatattgt
    38641 ttaaaatcta actttattag gtttgggatg atggacaaga ttttaattta aaagagtgca
    38701 tagatatata atggcataat gtggatttaa attaaaaaaa taaagccata aatattgaaa
    38761 aattcaatat ttatggcttt attgctttgt aataattaat agaatatata ttttttatta
    38821 atcatctatt tcaaattcta atattttacc agtaattgca ttaattgtta tctcatattc
    38881 agtttttcct tttattattt cacctttata ttctttgtca tcttcatcat atttaaattt
    38941 tactattttc ccaccaggca ctttatctag tattattgat ttagcttttt cagcactaat
    39001 aatagaactg ttgtcattag tattagagtt attattgtga ttattatcat tatcatcaat
    39061 gtcatcatca gaatcattgt ccatatcaat atctataatt cttccatcaa atgcacttat
    39121 ctctaattca tatgttttct tattttttat tactttagct tcatattcat tatcttccgc
    39181 atcatattta caatagatta aattaccatt aggaacagca tctagtgcta tagccttagc
    39241 tttatccaat gatatatttt ttgtacctaa attattatca tttaagtcat catctttttc
    39301 aatttctatt attttaccag agtaggcatc aatttctatc tcatatagaa cattatcctt
    39361 tacaactttg cagtcatatt cattatcatc ttcatcatag taacaaacta aaatatcact
    39421 tcctggaaga gtttgtttta atatttcttt agcttgtatt aaagatattt ttttagtttt
    39481 atttcttatg ttcacattct ttagttttaa aatatcttca aacgcttttt gattatatcc
    39541 tcctcctact tgtgttattt catctatatc tttattttca ataaattcct tttgactatt
    39601 tacaaggcta ccattagata tcattatagg agaattttct ttagcagcta gtactcctat
    39661 tgataatgaa tctactagtg cattttcttg gctaatacca tctttagcta caaagacatt
    39721 atttattttt ttatctttat aaaatttatc tataattttt gcattagtat tgtttctatc
    39781 tgaaccacta attctttctc tgtttggata tttctttact gcattatcag ataaaatctt
    39841 atttcctcca actatgtaag atttttttat agacctactg tttaagaaat ttacgctact
    39901 atcattgctg ttttcatctg atactaatat tataggcata ttatgtattg ctgctggaga
    39961 agctatacta actgcatcag ataatccctt tagaccattt acaactgcaa cattttctac
    40021 cttttttatc ttatctattt ctttaagtac atttgtagac atttcataga tattgtttcc
    40081 tactatttta tttattttta tgttaagcgc ctttatttca ttttctactt tagaagaaat
    40141 actatttcca ctatctacaa tgtacacatt gtctacacca agccttttaa gttcagcttt
    40201 agttgaactt tctatgctat tgttgtttac taaaagtata ggtgcattct ttagcttagc
    40261 aaatggattt gcacaaagag cactttgaat agagttacca ttaactataa tggctgtttt
    40321 tgattgaacc catcctaact tacttacttc tacagctgtc tcatatcttg ttttacctat
    40381 taatctttct tgctttgtag tattgtctgc aaaaatcgat gaagagctag aaaaaaacat
    40441 aatacccgat attaatatag ctaaattttt cttattcata tttttacctc acaatcttta
    40501 aaaatttttt atttagcata cttaataaaa tataatttta aaatcaaatt gatactgaat
    40561 ttgattttag aaaataaaca aataaaattt ctttgataca aaattttcct tttatattta
    40621 acattgtatc acaaatataa agaggaaaaa actttttaaa aagagtatac actattcatt
    40681 tttatcatct tataaataaa tctttaccac tgttttatat gttttaaaat agacacaaaa
    40741 cttatttcta aatttactac aaatatttta ggtgaaattt ctttttaagc atatctaaaa
    40801 aatatttgta gtaatgctaa gggattggat ttcttaaagt atatatttct ttggatgttt
    40861 gagtttgtga aatttgagaa ttggagggtg aaaggaacac tcaatggatt aatattaaaa
    40921 actacatttg tacaataatt gccttaaaac taaaaaaatc tattgacttg aagttaactc
    40981 caagtagtaa agtataatta tacaggaatt atatacttgt aagaatactt aaagtagaga
    41041 gataaaacaa ataagtttta aagggggaag taaaacaatg gagacaaaaa aattaggatt
    41101 tggattaatg cgtttaccaa taaaagatga aaatgatgtt acaactattg atatggaaca
    41161 tttgaataaa atggtagaca cttttttaga aaggggattt acatattttg atacagctta
    41221 tgtgtatcac atgggaaaaa gtgagatagc acttagagaa tctctagtaa agagacacaa
    41281 aagagagagt tttactgtag ctacaaaact tccattaatg gctttaaaga aaaaagaaga
    41341 gcaagaaacc atttttaatg agcaattaga aaaatgtggt gtagactatt ttgattacta
    41401 tcttttacac aatataggtg tgtcacatta tgaagtagcc aaaaaatttg attaaaaaat
    41461 agctacgcac actcgtgact ttagtcatga gttagtagct aaattagtca gcatataggg
    41521 aaacctatat gtagagatag gatagaacct atccaatact acttgaattg cta
//
LOCUS       DAMJVM010000026        38086 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.026, whole genome shotgun sequence.
ACCESSION   DAMJVM010000026 DAMJVM010000000
VERSION     DAMJVM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38086
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.026"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(105..1982)
                     /locus_tag="PC523_001471"
     CDS             complement(105..1982)
                     /locus_tag="PC523_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2475006.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKIEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNEVGKKNESEEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEEAVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVIMLPATLFLFGPIGIYIGNII
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AVGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSFIKVETKDGAIDITSPVEGECIELSEVKDDVFASKAMGEGIAILP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            2318..3505
                     /locus_tag="PC523_001472"
     CDS             2318..3505
                     /locus_tag="PC523_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2475007.1"
                     /translation="MKKTSNQKLLLLSIVTFFWFSQYVYIPFQSPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFFPEGNGFLLAN
                     ILSGISSSTWISYMVHYTNFFSKEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKMPKKISIKKYLSICKDKRLIFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVISSGFASTKFCTKYEPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQRSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIRYGYFVLAIIAIVASILSYTYYKKLDLTLNYDI
                     IQS"
     gene            3531..4226
                     /locus_tag="PC523_001473"
     CDS             3531..4226
                     /locus_tag="PC523_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF2475008.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKIDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATRLAILNATSKEIQKIKYTYLKSIEELDKVSQGLIEECYDADLQFHKEIILAS
                     HNPIFIRYYELIPKTISSNQYFGFKYRNSLKDMISHHNNIIIAFDSKDSNLGYTSMYN
                     HLEDVIQLFQHDD"
     gene            complement(4398..4700)
                     /locus_tag="PC523_001474"
     CDS             complement(4398..4700)
                     /locus_tag="PC523_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HDF2475009.1"
                     /translation="MNKKIVAVCESERSLNSLKMAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(4722..5132)
                     /locus_tag="PC523_001475"
     CDS             complement(4722..5132)
                     /locus_tag="PC523_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDF2475010.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            complement(5331..7259)
                     /locus_tag="PC523_001476"
     CDS             complement(5331..7259)
                     /locus_tag="PC523_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2475011.1"
                     /translation="MVWHIEYEVFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIEKIPIFITFLLNQIYSLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLLICIILSITNPFTHILFGLSKETGYIRGCGYVATFSSFFFYAIYSALL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLVHIHDVNHVYRIGGDIFSIVVNSQLGIKPEDLIKKIEERFKKTWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKRKHVIKNCL
                     EEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFITIAEQTGLINL
                     IGLVVFEKVCRFIASDEFEGLKLDNIAVNLSVVQCMQKNLADDLLYLMDKYNISPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIGYSGVSNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PIPEDAFKNLLKENAFTG"
     gene            complement(7322..7810)
                     /locus_tag="PC523_001477"
     CDS             complement(7322..7810)
                     /locus_tag="PC523_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017825269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2475012.1"
                     /translation="MKITILYQTRSGNTERVAKLIEDGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTVVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FN"
     gene            complement(7946..8956)
                     /locus_tag="PC523_001478"
     CDS             complement(7946..8956)
                     /locus_tag="PC523_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2475013.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGTALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGLALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALCDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     IF"
     gene            9444..9788
                     /locus_tag="PC523_001479"
     CDS             9444..9788
                     /locus_tag="PC523_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475014.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIEKFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            complement(10119..11495)
                     /locus_tag="PC523_001480"
     CDS             complement(10119..11495)
                     /locus_tag="PC523_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF2475015.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGHSIQSIIPNTKMLDVMRDKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENKDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(11594..12616)
                     /locus_tag="PC523_001481"
     CDS             complement(11594..12616)
                     /locus_tag="PC523_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2475016.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSMCDYGIVGDLYEIIPEM
                     IESLNR"
     gene            complement(12627..13421)
                     /locus_tag="PC523_001482"
     CDS             complement(12627..13421)
                     /locus_tag="PC523_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2475017.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKINVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            complement(13432..14574)
                     /locus_tag="PC523_001483"
     CDS             complement(13432..14574)
                     /locus_tag="PC523_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDF2475018.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASHTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            complement(14614..15846)
                     /locus_tag="PC523_001484"
     CDS             complement(14614..15846)
                     /locus_tag="PC523_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HDF2475019.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSKAGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            complement(15983..17323)
                     /locus_tag="PC523_001485"
     CDS             complement(15983..17323)
                     /locus_tag="PC523_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2475020.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFVVGSTILYKMVSKEKANGGHFIA
                     HESDVFDESEATNSKKENGPNGVIALIPLIISILIINVKINGQAIVPIEVGVFIGALL
                     VYLLLNKYQDNAKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            complement(17417..18211)
                     /locus_tag="PC523_001486"
     CDS             complement(17417..18211)
                     /locus_tag="PC523_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDF2475021.1"
                     /translation="MESIMENLNNLKVEVKDKVCVITINRPKALNALNNDTLRELSQV
                     IDVISENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESIKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKSALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            complement(18955..19674)
                     /locus_tag="PC523_001487"
     CDS             complement(18955..19674)
                     /locus_tag="PC523_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2475022.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKDGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(19757..20512)
                     /gene="nadE"
                     /locus_tag="PC523_001488"
     CDS             complement(19757..20512)
                     /gene="nadE"
                     /locus_tag="PC523_001488"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HDF2475023.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPSQPPKF"
     gene            20690..21076
                     /locus_tag="PC523_001489"
     CDS             20690..21076
                     /locus_tag="PC523_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDF2475024.1"
                     /translation="MKKTDGLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTTSSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            21076..21453
                     /locus_tag="PC523_001490"
     CDS             21076..21453
                     /locus_tag="PC523_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDF2475025.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGVF
                     LLLVGLFEVKKGFCAKSNKKPLN"
     gene            complement(21539..22183)
                     /locus_tag="PC523_001491"
     CDS             complement(21539..22183)
                     /locus_tag="PC523_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDF2475026.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVVVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLESGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTIYEDNVEIVVYSRLEDVDKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILL"
     gene            complement(22492..22935)
                     /locus_tag="PC523_001492"
     CDS             complement(22492..22935)
                     /locus_tag="PC523_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2475027.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVFAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            complement(23022..23699)
                     /locus_tag="PC523_001493"
     CDS             complement(23022..23699)
                     /locus_tag="PC523_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDF2475028.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDESSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            complement(23707..24990)
                     /gene="hflX"
                     /locus_tag="PC523_001494"
     CDS             complement(23707..24990)
                     /gene="hflX"
                     /locus_tag="PC523_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDF2475029.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTSESMEELKELTKAAGAE
                     VVGSLIQNKHSIDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGENGITLDVDLDEEDFNIYREYILEK"
     gene            25207..25467
                     /locus_tag="PC523_001495"
     CDS             25207..25467
                     /locus_tag="PC523_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HDF2475030.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCMLPHKKDNYNYIMGKFQDLVKITSKK"
     gene            complement(25573..26208)
                     /locus_tag="PC523_001496"
     CDS             complement(25573..26208)
                     /locus_tag="PC523_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDF2475031.1"
                     /translation="MKKFLNVLFSVCISIVIIVGVINFTVGFKKLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFNLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLIVSLVGIILSVKNKNIKILKTTSITLIMMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPNLDPVINILPEEFFFHSGMMILILILLSSILLFVMYKLCKYFKIK"
     gene            complement(26236..27786)
                     /locus_tag="PC523_001497"
     CDS             complement(26236..27786)
                     /locus_tag="PC523_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2475032.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQDYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            complement(27988..28872)
                     /locus_tag="PC523_001498"
     CDS             complement(27988..28872)
                     /locus_tag="PC523_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2475033.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKGENSNKNNKDKEEDKSNSKSNKDKLSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKNILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIIQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDEYFK"
     gene            29100..30107
                     /locus_tag="PC523_001499"
     CDS             29100..30107
                     /locus_tag="PC523_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HDF2475034.1"
                     /translation="MQLKNFHFKDNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNECKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLTQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            complement(30319..31017)
                     /gene="yunB"
                     /locus_tag="PC523_001500"
     CDS             complement(30319..31017)
                     /gene="yunB"
                     /locus_tag="PC523_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HDF2475035.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            31232..33925
                     /locus_tag="PC523_001501"
     CDS             31232..33925
                     /locus_tag="PC523_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDF2475036.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWDLISSDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLDLKYIKQSEYDEAIKAKIEIKLPKSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDKNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQST
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANQG
                     VYVEPSIFTTITTFDGQLLVKKAPEEHKVVDPEVAYVVTDMLESVITEGTGGVAALPN
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKVMQKIHANLTVTEFEVPKNVYFTKINLDDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQQQENNNNNNNSGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDNGGNSGGGSTNTPDGGGTPAT"
     gene            complement(34551..35486)
                     /locus_tag="PC523_001502"
     CDS             complement(34551..35486)
                     /locus_tag="PC523_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HDF2475037.1"
                     /translation="MILKQVIEVYDILDKADANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKSKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(35530..36702)
                     /locus_tag="PC523_001503"
     CDS             complement(35530..36702)
                     /locus_tag="PC523_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2475038.1"
                     /translation="MECFKEVVNMNELKVKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFSGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTSVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            complement(36689..37153)
                     /locus_tag="PC523_001504"
     CDS             complement(36689..37153)
                     /locus_tag="PC523_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475039.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLKLNGML"
     gene            complement(37168..38010)
                     /locus_tag="PC523_001505"
     CDS             complement(37168..38010)
                     /locus_tag="PC523_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HDF2475040.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTART
                     SNKL"
BASE COUNT        12480 a         6830 c         4484 g        14292 t
ORIGIN      
        1 aaataaattt actctatatt aattattttt ttaataattc attacaataa acttactttt
       61 agatgtattt aattgttaat ttacttatct ctagtcaaat taacttaatt accagctaat
      121 actaatgaaa ttatattatc attggttgaa acataactag atttacaaga aactacttgt
      181 ccaaagtcaa atgtattgtt tactataact ggcgtagtta catcataacc agcctttttt
      241 attttatcta tatcaaaaga tataagttta tcacctttct tcactaaatc tccttgattg
      301 acatgttttg taaagtattt accatttaac tctactgtat taattccaac atgtattaac
      361 atttctgctc cattatctag ttttaatcct attgcatgta gtgttggaaa taaagaagct
      421 acttcacaat cagtaggagc tgttattact ccttttgtag gtaatatggc tataccttct
      481 cccatagctt tagatgcaaa tacgtcatcc tttacctctg ataattctat acattctccc
      541 tccacaggtg aagttatatc aatagcacca tctttcgttt ctacttttat aaatgaattt
      601 tccttattag aagactcttc caaatcttca aatccaacta gataagttga aatagctgaa
      661 ccaaaaaatg ctatagcaca gccacctaga taacttaaga aacctaatgc acctgcttca
      721 gcatatacgg gtatagttaa aacaccttgg tttgcaaatg cattaccata agctccaccc
      781 attcccatta tggcacctcc aactgcacca catattacag cacataccat aggttttttt
      841 aatctaagat tggcaccgta tattgcaggt tcagttatac caaataaagc tgttatagtt
      901 gcagacatag aaattgtttt tagtccttga tttttagttt taaagaatac tcctagagca
      961 gctcctgctt gtgaaaagtt tgcagctcct gcaaatgcca ataaattttg tcttcctgtt
     1021 tgagcaacat cacttatacc tattggtaat agcgctctat gtgcaccaaa tataaccaac
     1081 acacaccata atccacctac aaatgctcca cacaatgctg ggcttaattc ataaatatac
     1141 ttataagaaa agtttatgat atttcctata taaattccga ttggtccaaa taaaaataat
     1201 gttgcaggta acattataac caatgataaa gttggaacca ttattatttt gattacctca
     1261 ggtatatact tttctaatac tttttcaaca tatgaaagag cccatatggc aataattatt
     1321 ggaataacag atgatgtata cgtagctttt gttactgcca agcctaaaaa tctaacagct
     1381 tcttcacctg ccatcaaagc agtaaaagtt ggataacaaa gtgtagctcc taataccata
     1441 gatattattt tatttgcatt aaatacttta gccgcagtat atcctagtat aataggcata
     1501 aaatagaata tagaatctgc catagcattt aaaataatat agctttgagt ttcttttata
     1561 tctactccat ttttattcat ataatacata gcaagaactg ataagatacc tttaatcata
     1621 ccaacacctg ctatagcagg tattgtaggt gtaaatatac cagctactgc tgataatata
     1681 gaattaatta taccttgctt ttcttcactt tcattctttt tacctacttc atttgattga
     1741 ggtacaattt ttataacctc gtcatatact ttttttactt tattgccaat tacaacttgt
     1801 atttgaccca tactctcaac aacagttatt actccttcaa ttttagataa tttatcctta
     1861 ttcactttag aagcactttt gactttaaat ctaagtcttg tcatacaatg agtcacttca
     1921 tttacattct cttttcctcc tacatttagt agaatttctt gagctatatt tttataatcc
     1981 ataaaaaatc ctccattttc aattatcggc tttttgtttt atggccattt aaacaaaaaa
     2041 atcgaaccaa ataaatagat tatacccata gtactctata gataatctat aaaaattgat
     2101 tcgatcttgc ctgcattacc agtaacacgt ctactgttat atattcaaat ttgttaactt
     2161 tatagtatca taagcgtttt cttaattcaa ctataaatta taattttttt gtaaataata
     2221 attatatatt taaatttttt ttaatagaga aagtacttga ttaaaaaatc taaatggtat
     2281 attatttgca ttataaatat accatatgga ggataatatg aaaaaaacat ctaatcaaaa
     2341 attactttta ctttctattg ttacgttttt ctggttttcg caatatgtat atataccttt
     2401 ccaaagtcca tatttaagtg catgcaatgt atctacaaat atgattggta taataatagg
     2461 tgcatatgga atctcacaat gtttactaag acttcctgtt ggagtatttg ctgattcaat
     2521 aaataaacat aaaatattta tactatctgg aattttcttt gctggtttgg catctcttat
     2581 aagagtattt tttcctgaag gcaatggttt tcttctagct aacattttat ctggaatatc
     2641 atcatcaact tggatttctt acatggttca ctataccaat tttttttcta aagaacatca
     2701 acaaaaagct acaagtacga taatactggt aaacaatcta ggaatgttga ttggatttgt
     2761 tatgagtact ttattttatg aaaaattagg aatgacagcc ttatgtatta tgagtgtatt
     2821 gtcttcagtt attggaacga ttctagcttt aaatataaaa aatcataatt caaaaaaaat
     2881 gcctaaaaaa atatcaatta aaaaatactt atctatatgt aaggataaaa gacttatatt
     2941 tttttccttt atggcattaa ttcaacaagg tattcaaatg tctacaacaa tgtcatttac
     3001 cactcaaatt cttaaaaact taggttctag ttctaccctt ataggtctat cttctatatt
     3061 ttacatggtg tgtgctgtta tttcttctgg ctttgcttct actaaatttt gtactaagta
     3121 tgaaccaaaa ttttggattc ctttagtttt tattttagtt tcagcctact gctttttagt
     3181 tccaacaata aaaaatatca atataatatt tgtactacaa gtgctaccag gtatatctac
     3241 aggaatactt ctttcttacc ttactagcga gtctatgaaa aatgtaccaa aacatcagag
     3301 gtctactgca atgggctttt ttcaagcaat ttatgctata ggaatgtctg tatttccaat
     3361 gatagttggt aaaatatcgg aatatacttc aatacgatat ggatactttg tcttagctat
     3421 tatagctata gtggcttcta tcttatctta tacatattat aaaaaattag atttaactct
     3481 taactatgat ataatacaat cataaaaaaa ttatatattt aggagttttt atgaaaagct
     3541 taaatgccac tgaaaaaatt attgattata ttaaattaaa cattctaaat ggaactttta
     3601 aaattaatag taaattacca agtgagagaa agattgctga cctatttaac ataagtagaa
     3661 tacctgtaag aaatgccata aatatactct gtaaggaagg aatacttagg gctgtgcctt
     3721 actcaagtcc tatagttgaa ggatttaaga aaattgacct atttgatgat ggtgaaatct
     3781 ataaaaatca taacatccaa gaattttatg tagaatcttt aagggcaagg caacttatcg
     3841 aaagtgaagc tactagacta gcaattttaa atgctacatc taaagaaatt caaaaaataa
     3901 aatatactta cctaaaatca atagaagaat tggataaggt ctcccaaggt ttgatagaag
     3961 aatgttatga tgctgactta caatttcaca aagaaataat tctagcctct cataacccaa
     4021 tttttataag gtattatgag ttaataccta aaacaatttc ttctaatcaa tactttggtt
     4081 ttaaatatag aaattctcta aaagatatga ttagccatca taataacata ataatagcct
     4141 ttgatagtaa agactctaac ttaggttata catcaatgta caatcattta gaagatgtca
     4201 ttcagctatt tcagcatgat gattaaactt ctatttttcc taagcacaac tgtcatacaa
     4261 aaatgaatta tctcaaaggg ttgtgtcaaa atagagataa tcacattttt attgttgaaa
     4321 tgataaaaat atatttgatt tcatagaaaa aaagtgtctc aaaatgaact aaaaagttca
     4381 ttttgagaca cccctaatta atttaagtct attaaaattt cacttactat agactttgca
     4441 tcctctatta tatactttgg atcaacttca taaaattctc tatctataaa tctatcaatc
     4501 ttctctatac tttctataga atcacttgta acaaataaca ctatagatgc gtcttctata
     4561 tcataaatag aaagttcatt ttttatatta tcattttctt gaatttcata aactaagtca
     4621 aaccctaaat catttgcagc catcttaaga gaatttaaac ttctttcact ttcacaaact
     4681 gctactatct ttttattcat atttcaaata cctttctaat tctactccgg tacaatcatc
     4741 actatacatt ttgcttgttt aactacactt gctgttactg aacctatcat tcttgtaaga
     4801 ccctttttag tagattttgt cataactata aaatctacat tatcttcttt tgcttttcta
     4861 attatctcgt ctcctggata tccaaaagta aaatgtacct taacatcata ctctcccatt
     4921 atctctgctg ctttatccaa gattcttctt cctaagtttt cagaattttt tatctcttca
     4981 gccagggata tgccatcaat aaatactagt tcttttacat tcattatttc tacttcaact
     5041 tcatcttttt tgaatattcc ttttataaat tccaaagaat gcatacttct ttctgtccca
     5101 tcaattggca ctaatatttt ctttttattc atgtcacatc ttcctttctc actttaggat
     5161 aattaaataa aatatatatt ctaagttata aaaatatata ataaatcttt acttcatgaa
     5221 ttaatattaa taatatgtat aataagttta atatttatta ttgtgttaat tttataatat
     5281 ataactggca gtgtcaatga acaaaattgt aattaaaaaa ataaagggta ttaacctgta
     5341 aaagcatttt ctttcaacaa atttttaaaa gcgtcttctg gaatcggttt agaaaaataa
     5401 tacccttgca agtattcaca ctccatagct attaaatctt cagcacattt tatggtttca
     5461 actccctctg ctacagcctt catattcaat ttatttataa gagctataat cgtttcaact
     5521 gtaagcttat gtctactgtc ttcattcata gaccaaatca agctcttatc taacttaatc
     5581 actgaaaacg gtaaagaaag catattagaa actccagaat aaccaattcc aaaatcatca
     5641 agtgcaaact taacacctaa atcaattaat ctttccattg tttctttaat tatattaaaa
     5701 gatcccatag ctactgtctc agtaatttca agtttaaatt tagttggtga tatattatat
     5761 ttatccataa gataaagcaa gtcatcagct aagttttttt gcatacattg tactacagag
     5821 agattcacag ctatattatc caattttaaa ccttcaaact catcactggc tataaatcta
     5881 cataccttct caaagactac taatccaatt aaattaatca accctgtttg ttctgcaatt
     5941 gtaataaatt catcaggtgg tataaaacct aactctttat gttccaacct aagtaatgcc
     6001 tctgctgaag taaacctacc tctgctatta gaaaatatag gttgataata cacttcaaat
     6061 ccatcatttt ttaatgcttc ttctaaacaa tttttaatca catgttttct ctttatcttt
     6121 tcacttatag acgcatctgc ataaacaact cgccttgact cagaacgttt tgcttcatgt
     6181 atagagaaat ctatagcaac aattacatct tctgtcgttt ctgcattttc tgggtaataa
     6241 acaacagcaa tgctagttgc taaattaaaa cttatctctt ctactagcca agttttttta
     6301 aatctttctt ctatcttttt gatgaggtcc tctggtttta tacctaattg actattgaca
     6361 acaatagaaa atatatctcc ccctattcta tatacatggt ttacatcatg tatatgaacc
     6421 aaatattttc caacttcttt taatgcaata tctccatttt ttgttccaaa agtttcattc
     6481 atgaacttaa agtcatcaag tgcaattgta ataataaatc ccgattgttc agtattgata
     6541 ttcaaatcta gctcttgaat aaaagattgt ctattgagta caccagtaag gtcatcaata
     6601 ataagacctc gattctgcat agaaagatat gtcaaaagta aactacatgc acatgcagtt
     6661 cctgtaagaa gatactgtaa aaaaattgcc tgtaagataa ttgccacaat tgaaactata
     6721 agaaaacctc taatagccca tatttttgat tcatgcattg cttttctata tttaaaagat
     6781 aaaagtgctg aatatattgc ataaaagaaa aaggatgaaa aagtagcaac atatccacaa
     6841 cctcgtatat aacctgtttc ttttgacaat ccaaatagaa tatgcgtaaa aggatttgta
     6901 atagataaga ttatacagat taaaagtggt atagataaaa ataataatct ttttttcata
     6961 taccttatat cttgataaat aatagccata acataaaatg atacaagtgc tgctggtatt
     7021 ggcaataaca atgagtatat ctgattcaat aaaaaagtaa taaaaattgg aattttctct
     7081 atattattta gacaaaatga acctaaaata tttgaaatta taaataaaaa tgagaatatt
     7141 aaaagtgcac aatatatttt gttttgtaaa gtaggtataa atttgcttct aaaaaagtat
     7201 accattaaaa aaataactat aattgcagaa aaaacctcat attctatatg ccataccata
     7261 aacttccctc actattgacg tttgatttta aatattttaa tcatcattat gcatatagaa
     7321 attaattaaa tagctctttt gccttagtag caattctttc accaaatatt atagccttct
     7381 catcttgtag ttctggacct tcaattgaat gtacatgtcc taaatgtata aatggttttc
     7441 ctaaagaacc acctccagaa tacacaagca ttcctttaac catcaaatgt cctactacag
     7501 taagtatagc aacatctggg cctccacata tataatcaca tgtagcaaaa acagctccta
     7561 attttccagc taaattgact ttttttgaat cttcatcaaa ccattttttt acttcccaag
     7621 ttgtatttgc taagtaagta ggtgttccaa atattactgc tttactttca tttaaataat
     7681 ctaaatcaat attatcaagt ctcataagtt taacatctac tccatctacc tttttagctc
     7741 catcttctat cagcttagct accctttctg tgtttccact tcttgtttgg tataatattg
     7801 taatcttcaa ctttaattcc cccttatcta ataaaaataa tttattatat atcaaattat
     7861 agcactaatt ttgctgtact acaaagaact cgtcgattat ttttttatta actatttccc
     7921 ataaaaaaca cctacatcta ttagcttaaa aaatacctaa gctcactttt aacttgctta
     7981 tttcaatctc aactatatct gatatatctt caaaagacat acctctatca taaagttttc
     8041 ctgcaatttt gatttttgca ttttcttcac cttttttaat caatgctcta ttttcttttt
     8101 ctattacatt tctaacatat cgctttgcaa atggttcatt attatcacat agagccttgt
     8161 atttttcctt gtctcctttt acaaaactaa gtgccctttt atcttgtttt atagcttctg
     8221 tacaaatttc ttccgtttgt tcttttacaa atttcaatgc cattccatat tgtcttactg
     8281 cctttaaaca tataccaaca gtctgttctt ttacaaattc taatgccaat ccattttgtc
     8341 ttactgctct taaacagatt gtttcagttt gattttttac aaatttcaaa tcaagcccat
     8401 tttgcctaac tgcttccata catatttctt ctgtttgatt ttctacccat tttagattat
     8461 agctacaatg tttaactgct tccatacata ttttttctgt ttgatttttc actcttgcca
     8521 atccaaattc actatttcta actgcttcta tacatatttc ttctgtttgt tcttttacat
     8581 actccaaagc agtaccattt ttttcaacag ctcttaggca caactcttct gtctgatttt
     8641 ctatgtattt taaggctgtt ccatcttggt taatagccaa catacaaatt tcttctgttg
     8701 gttcttccac aaattctaac attctccaat cttgtttaac cgcctctata catatttttt
     8761 ctgtctgatt ctgaacacat tttattgcat tgcaattttg tctaacagcc tctaggcata
     8821 tctcttctgt ttgatttttt atgtatatta aattcaatcc attttttctt actagctcta
     8881 aacattcttc ttctgtttta ttatcaaaat cctcagtatc tattgattca aatatgtcat
     8941 ttttatcttc atgcatatct tacattcacc ccttataaat tttataaaat tcaataaatt
     9001 tactctcaat tttattctat caaagaatat aaaaattttt gagtcttgtt ttttttcaca
     9061 gaatcatttg tatgttaaaa aataaattaa ttaatctaat tctagtatac aatacccaaa
     9121 atatgtggta tattttaact ataatatatt tatatatatt attgtcagaa tatttaaagt
     9181 taaatttata taaaacatct ttatttatat atcacttata tttggatata ttttcttttt
     9241 ttagttttcg ggttagtaag gggttagtag tttaggtttt cacaggatat tatttttaca
     9301 attagtttta tttttgtata atatatatat gttgcaatta ataaaactaa atatgattta
     9361 aataattcct taggataaat ttaataaaac ttagattcct tatctaagct taaaatacta
     9421 aaattattgg gaggatataa ataatggacc aaatcaaacg tttacatgaa ttgcaacaaa
     9481 aatcatattt gactaaagaa gaatttgagg aattaaaaaa taacacatat attgaaaaat
     9541 ttgaattaag agatgagtgc gataactctt atatttacac attttacact aacgacaaat
     9601 gtaatgatat aaaatttata aagtcacaat ttatcgttac acttataaaa attggtttcc
     9661 tatgtgagtg tggtggtgta tttagacaga agaaaatagt tgatgaatat gattacggat
     9721 ataatgctat atataagtgt gattcttgtg gaaaggaact tgataaagaa gtagaaaatt
     9781 atgattaaat aacaaaaaag ttattcaaaa tatttttata aatgctagat aaaattcact
     9841 ctaaatttga cctaaactat ccctcttagt agacagttaa aatatgaaaa ctgtccaaaa
     9901 ctaagagggt tttattctgt cctaaattaa acataataat cttagacatc aaaagataat
     9961 gaaatataga tagtaattat aaatacaata aaaaagtact aatgattgac actagtactt
    10021 cacaactact tttgcacatt ttaactacct aaaacagttt atacagttca ccttgtataa
    10081 tgttgattct tttgcacatt gctttaattc aagtcatctt aattatttaa ttcaatttca
    10141 tactttttta ttttcttata taaaagactc ttgtgtattc ccataacctc tgctgcctta
    10201 gaacatttcc aattgtactt ccttaacaca tcaataatta aactcttttc ataattctca
    10261 accatttcat gaattgaaca gttactatcc acattacatt gagctactct actgatatta
    10321 tccattttag atggaaggtc acacatcata atgaccatat catcacttac agcataggca
    10381 ctttttatta cattttgtag ttctcttata tttcctggcc aagtataatt aataaaacaa
    10441 tttttaacct tatcacttag aaccttcaat tccttgtatt caacatttaa tttgtttaat
    10501 atataatttg ctaacaataa aatatcatct tgcctgtcct tcaattccat catatgaatg
    10561 tttattacat ttaatctata gtataggtct tctctaaata atccttcttc aaccatttta
    10621 cttagattct ttcttgtagc agagattact cttacatcta tctttatggt tttaagacct
    10681 cctactggtt ctatctcact ttcctgaaga actcttaaaa gttttgcttg catagtcaaa
    10741 ggcatgtcac ctatttcatc taaaaagatt gtaccattat tcgcaactaa aaacttgcct
    10801 tttttaccac ctttttttgc tcctgtaaaa gctccttcat cataaccaaa taactctgat
    10861 tctaataatt cttctggtat tgcagcacag tttatactaa ccataggttt gtctgaccta
    10921 ctgctattat tgtgaattgc tctagcaaat acttctttcc ctgtgcctgt ttcacctgtt
    10981 aaaagtactg gaaagtttgt tttagaagct ttcacacctg ttttcttaag ttctataaaa
    11041 tctttatttt cacctatgag cttgtcaaaa ccacaattat ccttaaactg ttctttataa
    11101 tattgaagct ccatatattc tgacattaac tttttagcca catcaaagga ttgcaatcta
    11161 aatttaactt gagctactcc tgcaactact tctccattgt cattttccac ataagaccta
    11221 cttactatca cacttttttc ttttgtattt ccacctgaat aatgatgtat agctccttct
    11281 tcacagtact tatctctcat aacgtctaac atttttgtat ttggtataat actttgtata
    11341 ctgtgtccta ttatatcttt tctttctttt cctaaaaagt cacaatattt atcattaata
    11401 tctattatta ctccacttgt atttacaaca ataaaaccat catcagtcat agataatact
    11461 cttgcaaata tatccttata ccactgttct ttcattgttt catctcccca ctataacctt
    11521 cagcaaaaat aataagtctt acaatacaat attataagac ttatcattct acatattcaa
    11581 tgttttctag aagttaccta tttaatgatt ctatcatttc aggtataatt tcatataaat
    11641 ctcctactat tccatagtca cacatactaa aaataggggc ttttgcatct ttatttatag
    11701 caactatata tttagaatca ctcatacctg ctaaatgttg tattgctcca gatattccac
    11761 atgcaatgta tagttctggt ctaacagtag ttcctgtttg tcccacttga tgagaatgtt
    11821 ctatccaccc tgaatctact gctgctcttg aagcaccaat ctctgccccc aatttatcag
    11881 ccaattcttt tataagctca aatccttctg ctctttttaa tcctcttcct cctgatacta
    11941 ttattctagc atcagttaga tttacagatt tcttttcttg tggaagtatc tctactaatc
    12001 tagttctaat cattttttca gtaagttctg gagtaacaac ttctaaaatt ccagtctctg
    12061 attcatttct tacagctttt gccataactc ctgggcgaac tgttgacatc tgtggtctat
    12121 ttttaggaca aacaatagtg gccattaaat ttccaccaaa tgctggtctt gtttgtaata
    12181 acttattatc tgttgaatct atttcaagtt ttgtacagtc agcagttaga cctgttccaa
    12241 ctttacctgc aattctaggc gctatatctc ttcctataga agtagctcca actaatacaa
    12301 cctctggctt ttttctttcg attaagtttg caattgaaat tgcatatcca tcagtagtga
    12361 aatctttaag aagagggtca ttgatataat atactttatc aacactatag tgaagtaatt
    12421 ccttcacctc tttctctact tcatatccag ctatcacaac tgcaagctct tttcctaatt
    12481 ggtcagcaag ctttcttcct tcaccaataa gttctatagt aacaggatta attttgcctt
    12541 ctctttgctc tcctattacc caaacaccat tataatcatt taaatttata ccttgattaa
    12601 cttttgctct catcatctta acactcctat actagtttca aatcgaatag tatttctatt
    12661 agtttctcag ctttttcttt tttatttata ccttctatta tttcattatg cttgtcttta
    12721 actggtacaa aagacttata aacatttgta ggtgaaccct ttaatccaat ttgagttgtg
    12781 tcaacatcta aatctgcaag tgttaacaca ttaatctttg catctccatt attataagct
    12841 tctaagattc ctttgacact tggatatctt ggattattta actcttttat agcagttagt
    12901 aaaactggca tctttgcttc gattaaataa tctcctgtct ctgtataacg agttactagt
    12961 aatttgtcat cttgaacttt tacttctttt gcataagtaa cttgtggtat tcctaaaaat
    13021 tctgcaacct ctggtccaac ctgagctgta tctccatcta tagcttgtct tccgcagaat
    13081 attacatcat acttcccaac cttctctata gcagcagcaa taattgtaga tgtagcccaa
    13141 gtatctgagc caccaaaagc tctatctgaa attaaatatg cttcatcagc acccattgcc
    13201 aaagcttctt ttaaagcact tttagcttga ggaggtccca tgcttataac cttaatcact
    13261 gctcctaatt catctttcat ttttaaagct tcttctaagg catttctatc atctggattg
    13321 attatacttg gtacaccatc tcttattaga gtacctgtct ctttatttat tttaacttca
    13381 ttagtatctg gaacttgctt taagcaaaca actatattca ttctcttcct cctatcctag
    13441 tactgctctt gaaataacaa ctttgtgtat ttctgaagta ccttcgtaaa tttctgttat
    13501 tttggcgtct ctgtacattc tctctaatgg ataatccttc atatatccat atcccccatg
    13561 gatttgaaga gctagatttg taacctctct tgcagtatgt gatgcattgt attttgccat
    13621 ggcagcttct ttagtgtgtt tttctcctct agctttcaag tctgcagcat aatatactaa
    13681 caattttgct gattccacca ttgtcgccat atctgctata taccattgaa taccttgcaa
    13741 ggctgaaatc ggcttgccaa actgaactct ttctttagaa tatttgatac tttcttctaa
    13801 ggctccttca gcaattccca cagcttgagc tccaactcca attcttgctc catctaaaca
    13861 ggtcatagct atgttaaatc ctttgccttc ttttcctaat aagttagact ttggaactct
    13921 acagttatca aatattaact ctgcagtttc tgaaccatga atacccattt tttcttcaat
    13981 ttttcctatt gaaaaacctg gagtcccttt gtcaacaatg atagcagaca ttccctttat
    14041 tccctttgat gggtctgtca gtgcaaatat tattaatgat tctgcttgac ctccaccaga
    14101 aataaaacac ttagttccat ttaaaacata ctcatcagtt tcttcatcaa taattgcagt
    14161 tgttgcagct cttgcagcat ctgaaccagc attaggttca gttaaagcaa aagcacctac
    14221 acacccacct tcagccatca ttggaaggta ttttttcttt tgttcctcag taccaaattt
    14281 caaaatagct tgagcatata ttgtgtgtat tgataatatt gctgcagatg ctgcacattt
    14341 tttggcaatc tcagtaacaa caattacctt ttctatatct gctccaccag aaccactata
    14401 ctctacaggt gttccaatac cattaaatcc tattttagcc attttgctaa aaagctccct
    14461 tggaaaaata cctgttttat ctgtttcagc agctattggt gctatttcat tctctgcaaa
    14521 ttcttttgct actctttgaa gcattaattg tgcttctgtt aatctaaaat ccataaaatt
    14581 ccctcctatt tattaacata ctccgcatgt ttattaacat atttcacatg cttctttatt
    14641 cattgttaca attccagatt caattagagc ttctatatca gaatcactgt atccaagttc
    14701 tctcaatatc tcatctgaat gttctcctat aagtggagca tcacaagtca tgttaacttc
    14761 aatatttcca aatttaactg gagtagatgc aagcattgtc ttatttccat ttttacttgt
    14821 atgctcatat atgtaattat tagcaatagc ctgttcatca gttactacat cattaacatg
    14881 ttgaattttt tcatgggcta tatcagcagc agtaagcttt tcaaccatct cattttgagt
    14941 aaactttgca aattcgccat ccaaaatatt aataagttct gtagcatttt tcttactctc
    15001 tactgaagaa gcaaatttct cttcttttat taagtcttct ctaccaaata atctacaaag
    15061 tgcctcaaaa tatctttcat gctctaaaat acttaagaat atccactttc catccttaca
    15121 acgataagaa tttattactg gagaaatagc attttttcta gtttttggat actcatcgct
    15181 aaactgtgta gatgctaata aagatgatgc attccatatt gcttgtccaa ataaagatgc
    15241 cattactttt tgtccttttc cagtcttagc ttgctggtac aaagctgcac atattcctcc
    15301 agataaacta caagacgttg ttgcatctcc aaaagctata ggtggattta ttgggcttgt
    15361 atctttttct gctatatcaa gcatagctcc acttcttgcc caaaaggcaa ctgtatcaaa
    15421 tcctgcatta tctttagcag gtccaaagtc gccaaaacca tttatctgac cccagataag
    15481 gtgtggatgc tttttactta atgattcata gtcaagacct agtcttttta atgctccggt
    15541 tctataactt gaaacaaata cgtttgcttc acttaaaagt ttatccataa tttcaattcc
    15601 agctttgctc ttaagattta tagatagacc tcttttattt gaattatatg tactgtaaaa
    15661 aggattatta taatcatcta ttggcattgt cattgttgct cctgtctttc ttcctgggtc
    15721 tccaaagctt ggctctacct taataacatc agctccccag tcagctaata ttttagaagt
    15781 tcctggacct gctacaaaat acgttaagtc aataacctta actccttcta atggtttatt
    15841 taacactttc aattcctcct tatatggtca ttttgttatt ttaaatacaa acttacagta
    15901 ttaaaacatg aaatttattg tatatacttt gaaaattaat tttacaatat caaaatataa
    15961 tttatgcatt ttatcttctt tcttatggta atcctgggaa cattgtaaac agtaagactg
    16021 ctactatggt tccaataaac ggagttaaaa cagttaccca gaatattggc atataggcat
    16081 ccttatgtgt ttctttacac agaccatttg ttacagtaac gatatatcca ttgtgtggaa
    16141 gtgaatctaa tgctgctgat gaaattgcca tagtcctagt taatgctgca gcagaaacac
    16201 ccttagctaa aaatactggt ccaagaagtg gagctgcaat tcctagtcca cctgaagctg
    16261 acccacagat acctgcaata actgtagttc caactgcagc gcctgcaata ggaggaccag
    16321 gaatattagt catggcatta actatgaagt caaaaccaac tgctgatttt actactgaac
    16381 caaatgcaac tacagcgcaa gtattacata ttgctactac tgtagtttta catgcatcac
    16441 caacatgtcc aactatttta gcattatctt gatatttatt taacaataag tacactaata
    16501 aagctccaat aaatactcct acctcaattg gtactattgc ttgtccatta atttttacat
    16561 taattattaa aattgatatt attaatggaa ttaacgcaat tacaccatta ggaccatttt
    16621 ctttcttaga attagttgct tctgattcat caaatacatc tgattcatgt gctataaaat
    16681 gaccaccatt ggccttttct tttgaaacca ttttgtacaa aatagtgctt ccaactacaa
    16741 acattactgc acatgatata aaaccattta ctgctccagc cataatatct gtccccaatg
    16801 tcgaggctgg gacaatattt tgaatttgtg gtgcgcctgg tgcaaccata gcaaaagttg
    16861 aacatccaaa agtcaacgct gctggtataa atcttcgtgg caaatcagct tctttaaaaa
    16921 tttctaaagc tatcggaaac actgcaaaac ttacaacgaa aacacttact ccaccatagg
    16981 ctaagattcc acaagctatt ggaatagaaa gtaatgcttt atccttccca atccacctta
    17041 caatcatctt agcaatagac tttgctccac cagtaatctc cattattttt cccattaatg
    17101 tacctgttag gaaaataaag aaattcgcct gaagaaatcc aacaaaccct gtcatataat
    17161 ccaccttaag cgcatcatac acatttagac ttcctgttat agcaactaat gatgaacata
    17221 caattgctat tataaaaata ttgattccct tcataaccat tacaactaga agagatagag
    17281 ctatgataat ccctactaaa cttataatta ctcccgtatt cataaattaa ctccttttaa
    17341 aattaaacta tatgtatttt ttcacgaaat ttagattttg aatatattct acattgttcc
    17401 aatttagttg taatagctac ttattttcaa aaactggctt tctcttttct ataaatgcat
    17461 tcattccttc ttgcttatct cttgtagcaa aagttaaacc tgcaatatct ttctctagtt
    17521 caagagcact ctttaaatcc atatctatcc ccttgttaat aacaactttt gcatatctta
    17581 ttgctattgg cgctttttca actatttgat tcatcatctt tatagactct tctaaaagta
    17641 aatcacttgg aaccacttta tttataagcc caatagattt agcttcttct gcatttactt
    17701 gtctccccgt aaaaattaac tctttggcta ttccagttcc aacaagtctt gataatcttt
    17761 gagttccacc aaaacatgga ataactccta aattaacttc aggttgtcca aatatagcct
    17821 tttctgatgc aattctaata tcacaactca tagcaagttc acatccacca cctaatgcat
    17881 atccattaac tgctgctatt actgtcttct caagagcttc aattttatca aaaattccct
    17941 gagcataacc tgcatatttt ctgccttcct cacttttata gttttgcatc tgacgtatat
    18001 cagccccagc tacaaaaact ttaccttctc cagtaataat gactcctaat attgcctcat
    18061 tttctgatat tacatcaata acttgtgata gttccctaag ggtgtcatta ttaagagcat
    18121 ttaaagcttt gggtctattt atagtaataa cacagacttt gtccttaacc tccaccttaa
    18181 gattgttcaa attttccatt atagattcca tcatattccc cccattatac attgttattt
    18241 tttctacatt tcttgttaat ataaaatagc aagtactatg ccaatcacca gtgaagctct
    18301 ttatttttaa taaaataaac gaaatataca taaatttcac taaattttta ataattttta
    18361 actttaaaat tgattaaatt gtaattcaaa aaagaatttt tcttcaataa aactcataaa
    18421 acaatatttt tgtctcattt tcgagaaaat acctaaaaaa cacagtctca tttttgagaa
    18481 tgcagtctca tttttgatat ttctattaaa atattttaag cagtatatct ataagtttac
    18541 ttcactctaa tataaggaat ctttattcaa catagtagat aatttatttt ctattgactt
    18601 aaatcattaa tactttagtt caatatatat ctattctaat agtacaaatt tatcaataaa
    18661 aataggaaaa accacaaaga cttaatgtct aagtaacttt tcccatctta aacgtttctg
    18721 acaattttag atttgatttg caatgttaag ttaatctcat ttgatattat tctaaatcaa
    18781 ataactccgt tttcttttaa atttcttaac ctataaatat aaaaacacct cttacctatt
    18841 atgcaaatat aggttagagg tgtatatgtt tattttagta atttaatatg ttataaaatt
    18901 tatcacatat taaaattaat gcaatactta taaacttcaa tatacttata ctatctattc
    18961 tacttcccag ttatgatata tatcttgaac atcatcatct tcttcaagca tatcaactaa
    19021 tttattcatc atttttaaat gagattcttc agtcaataca gtcgtagttt gaggtatcat
    19081 cttcacatca gcagatataa attcatatcc tttagctttt aactcatcac gaacactaga
    19141 aaaatcttct ggtgtagtta ttatttcata accatcttct tctgttataa aatcttcagc
    19201 tccagcttct aatgcaacat ccatcaattc ttcttctgaa actccatctc ctaaaccaac
    19261 taatatttgt ccttttttat caaacataaa agacacacat ccactagttc ctaagtttcc
    19321 tccgtttttg tcaaaatagt atcttacatt accagcagtt ctgtttttat tgtcagttaa
    19381 agtttctact ataacagcaa ctccaccagg tccatatcct tcataaacta tagtttcata
    19441 gtcttctcca gcacctgctc cagcaccttt agcaatagct ctatctatat tatcatttgg
    19501 catattgtct gccttagctt tttctatagc tgtttttaac gctgcattgt actctgggtc
    19561 tgctccacca tctttagctg caactgctat tgctctagca tgtttagtaa atatctttgc
    19621 tctttgagca tcttgtttac cttttctatt aattatatta cctatacgtc ccatatttcc
    19681 actccttaat acgtacaatt aattattcta attttcatca cttgtaaatt ttatcacaaa
    19741 tcttataaat agtaaattag aacttaggag gttgtgaagg cgtttttctc ttatgttcac
    19801 ttaatctatg aagtctttca attatctctt ggtctctttt aggaacttca tccaatctgc
    19861 cttctaacac agcatctatc atattataag tagttcccat ttcatcttca tctgtttgac
    19921 cttcccaaag acctgctgaa ggagctttgt ttattaaatc ttcatgaaca ccaagctctt
    19981 ttgcccattc atacacttct cctttagtta aatttgcaat aggtaaaata tcaactccac
    20041 catcaccaaa tttagtaaaa tatcctgtgt gtatttctgc agcattgtct gtaccaacaa
    20101 ctaagtatcc tagattgtta gcaattgtat aaatagtact cattcttact cttgctctta
    20161 agtttgcatc tgtcatcttt aaatattctt ctctatataa atgtttatct tttaaatttc
    20221 ctactaccat gtctaatata gcactttgtg gttctataag gtctaaatca agatattcta
    20281 tatcacatcc ctctataacc ttaagtgcat cttctctatc ttgaggatta ctttttatgc
    20341 tcattataac tcccatagag ttttctggaa atgccttttt aattaaatta gcaactactg
    20401 ctgaatcaat tcctccagat acaccaacaa ttaatccttt tgcatttgcc tcattaacct
    20461 tatttataag ccattctaca gttttgtcta tttgtatttt tatatttgac attgttatct
    20521 cctctcaaat ttgctacttt gttttaatat attataccat atattaatat gttgttttaa
    20581 tatgtttttt ttatgcaaaa ttaaacaaat atgaaagatt atatttaatc ttattttata
    20641 taaatataat ttgcctattt tggtaaaact atttcaaagg gtgattttta tgaaaaaaac
    20701 tgatggttta aagaaaaaat actttgattt taacttaaaa aatacgataa ttggagtttt
    20761 tacaggattt ataaatggag tttttggttc tggaggaggc actcttttag ttccaatatt
    20821 aaatgatatc gttaaggtag aagaacataa atctcatgca actgctcttt ctataataat
    20881 attcttaacg acaacaagtt ctgttttata tgtatctaaa ggtacttacg acgttaattt
    20941 aacaattaaa gttgcagtcg gaagtattct tggtggtatt ataggagcta aattattaaa
    21001 taaggttact ggaaagtttc ttagaatttc ttttggaata attatgataa ttgcagcatt
    21061 aaggatggtg ttttaatgtt atttgctcta attggatttt ttgcaggaat tattggagga
    21121 atgggaatgg gtggaggaac aatccttata cctgcactcg ttttacttgc tggcatagac
    21181 actaaaatag cacaaagcgt aaatttactt tcatctatcc ctatgacact tatagcactt
    21241 gctatacata taaaaaataa aaatgtaatt tttaaattgg ttatacctat agctgtgttt
    21301 ggggttttag gagctgtatt tggttcacta ttagctaatt atctatcttc agatatatta
    21361 aaaaaaatat ttggggtatt tttactttta gttggtttat ttgaagtaaa aaaaggattc
    21421 tgtgcaaaat ctaataaaaa gccattaaat tgatttaggt taaatcaatt taatggcttt
    21481 ttacttaaaa ataccaatta attatttaag atattgacta ttacaataac cttttagatt
    21541 atagtaaaat ttctccattt tgttcagaca aataaaattc ctctcctaaa actatttttc
    21601 cagttccact tgttatatca atcatttttt ctgataattt atcaacatct tctaatcttg
    21661 aatatactac tatctcaaca ttatcttcat atatagtatc ttttataaaa taccccaaat
    21721 tcattaactc attttgtact tttccaagtt gagtatagtc tatcttaacc ttaacttctt
    21781 gatacattac cttatagatt atcttgcctg attcaagtcc taacttagca ccttttgtat
    21841 aagctctaac tagacctcct gcacctaatt ttgttcctcc aaaataccta gtaacaacaa
    21901 ccactacatc tctcaaatct tcttttttta taacctctaa tgtaggtatc ccagctgttc
    21961 cttgtggttc tccatcatca ctatatcttt gtatattcat atttttacct acagtataag
    22021 cccacacatt gtgtgtcgca tctttatgct ttttctttat ttcatttata aactctaagg
    22081 cttcctcttc actctttata ggcttagcat atccaataaa aacagacttt tcaatcgtta
    22141 tttcatccgt tccaaattca tgtaatgtcc tgtaattgct cattatactt cctcctattt
    22201 taatcatttt ctaatataca catcaaatac atatttattt ataaaattat tctataaaaa
    22261 aataaatgga cttttaatat ttctttaatc aatatcataa gacaattata taataaaatt
    22321 gtatactttt taatttttat tttaaatata gcttactgat tttaaatgta aattactaaa
    22381 gctctaaatt gataaatcat aacttaaaca agctatataa tttatgctta aaactgcttc
    22441 aattaaaaaa ggaaggggct atcccttcct cagaatcctc taaattcaca attaatttgc
    22501 tggaacatca tcgccctctg aaactaattt ctttaattcc ttaaactttt tataagatac
    22561 ttctactaaa gcttgctctt tatgatgtga taacatttct aaagcatcct ttactttata
    22621 aaatccacct tcttgaaatt caggtgcaaa tacacagtca gtgttacaag cttccataac
    22681 ataccacatt attgcattac aaacttcttg ttgtctactt cttgagaaaa attcatacat
    22741 agtatcgcca gccacatcta tcattttagc atctatgcca gtttctactt taactctttg
    22801 aacagcactc tcatatggaa gacctcctcc tagtatcttt ccttttggaa gtgtccattc
    22861 acccctatca ttttttacaa taagtacttt attagcataa aacactactc cacctgcaca
    22921 acgtcttact atcatttgaa acccctccca ttaatttatt tattaccttg ttaaacttta
    22981 gaaataacat cattaagtaa acaaattgct ttaataaaaa attacttttt caaatttttt
    23041 gcattttcta atgacttaat ggcttcgtca taagctctaa gcacatattc tttttcaaaa
    23101 ttcttattct tgtctttcaa ctgttgtaaa atttttaaac tgctttcatc tcctaatttt
    23161 gaaagagctt ttgcacaata ctgcctagtc tgtgcatttt tgtcgtagag acctttttgt
    23221 agtacttctc tactacttgg tgattcaatc tttctgattg ctgaataagt tattcttcta
    23281 atatctgagt gtctgtggtt tgtgagctga tgaagcaatt tcaaaaatct atcatctttt
    23341 agctcacctg tcgcccaaat taaaacaatt aaatcttcag catttttttc tacaaggatt
    23401 cttttgaaaa cctttctctt aaagtatacc attttttcgt cgtctaagct atccataaaa
    23461 tcaagtctct taattttatc tagttctaaa aatttatcta aaatatcctt agacgcctca
    23521 aaatcatctt tttgcatttc ctttattttt aattttgctt gtataagttg ctcacttgct
    23581 tcagtacttg aaatatttct aattttacta atttgagaca ctgttttaga ttctttataa
    23641 agaagatagg ttataaaata atcttcaagg ttatctatat tttcccagtt tatatccata
    23701 ttcaacctat ttttctagta tgtactctct atagatattg aaatcttctt catctaaatc
    23761 tacatctagc gttataccat tttccccata ctcaaaattt tccacattat atttatcttt
    23821 tattctacta aatatatcac ctctctcata aggtaacatc aagcttactg aataagtatt
    23881 ttccattaaa gcaatttcta tcatatttaa taacttatcc atatttatgc cttgttttgc
    23941 agaaatatac acaatatctt cttgactttt aggatatata tccaattcta gcttgtccac
    24001 tttattatat acaagtatat gttttttatc atttacacca agttctttta atacaccttc
    24061 agtcgtactt ttttgcaatt cataactagt atttgtcgca tcaattacat gtaagattaa
    24121 atctgcatac tgtacttctt ccaatgttgc tttaaacgct tctactaaat catggggaag
    24181 tttacttaca aatccaacag tatctactac taaaaattct tttttattcg gcaaaagagc
    24241 ctttctcaag gttacatcta aagttgcaaa aagcatatct tttacaaaaa cttccttttc
    24301 ctgttcataa tccttgtgtg tctttattaa ttcgttcaac agggtagatt ttcctgcatt
    24361 tgtataacca acaagtgcta caatcggtat atttgacttt aaacgcttaa ctctctgtgt
    24421 ttctctattc ttttttactt ctttaagttc acgccttata tctgctgctt tattaagtat
    24481 atgtctctta tctatttcta gtttttgttc tccaggacct ctagtaccaa ttcctgcacc
    24541 tgttctactc atctcaccac ccattccata aagtcgtggt aatctgtatt ttaattgtgc
    24601 taattcaact tgtaatttac cttctttact aagtgccctt tgagcaaata tatctaaaat
    24661 taaagtagtt ctatctatta cttttctacc aacaacattc tctatatttc tgatttgagc
    24721 accagataat tcatcattaa aaattactaa agtagcatct aaagaatcac tatacgctct
    24781 tatttcttct accttacctt ttcctatata ataagctgca tctatagaat gtttattttg
    24841 aattaagcta cctacaactt ctgccccagc agcctttgtt agttccttta attcttccat
    24901 agattcacta gtgtctatat catcattttt tttcactatt gtagtcaaat tcaaacctac
    24961 aagaagtgct ttttcttgaa tttctttcac ttaatcacct cattttttgt tataaagcat
    25021 attctaatta caaaatacac ttacattact tttacattat ttttctgttg actaattata
    25081 ccattaatac tatgattaat aatactatat tatcgactat tcataaaatt atttagtata
    25141 attatattat caccaaaatc ttatatagta taaaaaattt aaattttatt taattaggag
    25201 gaagatatgg gcgaaaatta taagtttttt aatcacaaag attgtgaatt tttcccttgt
    25261 cataaaacta ataaaccaga agagtttaac tgtctatttt gctattgtcc tttgtatgct
    25321 ttaggagaga attgtggtgg aaactttaag tatacagata aaggtatcaa agattgcagt
    25381 agttgcatgt tacctcataa aaaagataat tacaattaca ttatgggaaa attccaagat
    25441 ttagttaaaa tcactagtaa aaagtaattt ctattggaaa tagctatgca taaatattta
    25501 ttgactactc ttagcctaaa tattattaca tagctatttt tattgtataa cctttaatat
    25561 aatttgtttt tgttatttta ttttaaaata tttacataat ttatacatta caaatagtaa
    25621 aatacttgac aataaaatta atattaaaat catcattcct gagtgaaaaa aaaattcctc
    25681 tggcaatata tttataactg ggtctaaatt gggattaaat atccaataat catttctaaa
    25741 taataattta tgaaaaataa taaatgactt ttcaaaattc aataaaattg gaactgttaa
    25801 taataatggc atcattataa gtgttataga tgtagttttc aagattttta tatttttatt
    25861 ttttacactc aatataattc ctactaaaga aactatcaac aataatttag ctaatttatt
    25921 tatattttga aatatattcc taacttcttc aaaatgtatc ttcccttgtg gagaagattt
    25981 taatgttggt aaattaaact ctgatacatt cttatttaag ttataatcga ttagataatc
    26041 gtagttcaat tttatatcat ctttagataa accagataac tctgatatgt ttaaataatc
    26101 aatgtcaaaa taataaagtt ttttaaatcc aacagtaaaa ttaataacac ctactattat
    26161 aacaatacta atacaaactg aaaaaagtac atttagaaac tttttcataa aatcccccca
    26221 gtaaaagtta aatcactaca cgattttagt tgtaaaatct tctatattcc atacatcatc
    26281 aacaatttct gaataaaatt ctggctcatg acataccaaa agaactgttc ccttaaactc
    26341 tttaatagcc ttcttcaatt cgtcttttgc ctcaacatct aagtgatttg taggctcatc
    26401 taatactaag aaatttatat tttttagcat tactttgcaa agtctaacct tagcagcttc
    26461 tccaccactt aatactctca tttggctagt tatatgttca tttgtaagtc cacattttgc
    26521 aagagcctgt ctaacttcaa agtttgtaag ccctggaaat tcactccaaa cttcatccat
    26581 aggtgtatta ctattttctc ttgagctctc ttgttcaaaa tatccaactt ctaaatagtc
    26641 acctagttta acatttcctt caaatggttt tattatccca agtaaagttt ttaatagagt
    26701 agattttcca ataccattca ttccttttaa ggcaatcttt tttcctcttt ctaaatggaa
    26761 attaagtggt tttgtcaatg cttcatcata tcctaaaact aaattttctg tttcaaaaat
    26821 tattttacta gctgctctag catctttaaa agcaaaagta ggttttattt tttcttttgg
    26881 tctttctaat atttccatct tatcaagttg cttttgtcta ctgttagcca ttcctctagt
    26941 tgctactcta gctttatttc tagctacaaa atcctctagt ctttttcttt cttcaacttg
    27001 tttatcataa gcttgttctt cttgacgttt ctttatatca ttaagtctta caaattcatc
    27061 atagtttcct ttatatctat ttagctctcc attttccata tgatatatta cattacatgt
    27121 attgtttata aattcaatat catgagaaac taagacaaag ctattctcat agtcttgaag
    27181 gtacttagta agccatgtta tatgctcttc gtctaaatag tttgtaggct cgtccaatat
    27241 aagaatagtt ggattttcaa gtagaagttt agtaagaagt acttttgttc tttgccctcc
    27301 acttaagtca gttacatctt tatcaagacc aatttctcca agaccaagac catttgcaac
    27361 ttcttgtatc tttgcatcta tcatataaaa accactattt tcaagtatag tctgtatttc
    27421 tgctgtttct tcaagaagct tactcatctc atcatcacta gcttctccca ttttgtcata
    27481 catagcaatc atttcttgct ctaagtcaaa catatgctta aatgcttctc taagcacttc
    27541 tcttatagtt ttccccttgc ttaataccgt atgttggtct aagtagccta ctgtagctct
    27601 tgaagaccat tttatatttc cagcatctgg cataagcttc tttgttatta tatttaaaaa
    27661 actcgatttt ccttctccat tagcaccaac tagtgcaata tgttcacctt ttctaagtct
    27721 aaaggataca ttttctaata ttgttctcgc accaaatcca tgactcacat tttctactac
    27781 tagcatttca acacctctca aaaaaatttt acacattcta tactataata tattcttttt
    27841 aaacttaaat caagcattac caatattttt agttatattt acgacaattt aacctaaaat
    27901 aaacttcact tttgtgttat gtacaataaa aaatagagat aaaaattagg aaaataatac
    27961 caacctaatt ttttctctat tcatactcta tttaaaatac tcatctaatc catcagctat
    28021 acactgcatc atttttcttt gatagtctgg atttgacatc atttggtctt cattatagtt
    28081 actcataaag cccatttcta ctaatacagc tggaactttt gaccagttga atccagtcaa
    28141 atcacctctt tgaatgattc cattgatttg tatacctgat tgttccatat tctgtttgat
    28201 aaattcagca catttattac tttcttcata tataggagcg gtatattttc catctttttc
    28261 aggaatcaat atagatgctc cagtttttga ggaattattt aaactatctg catgtattct
    28321 tacaaccata tctgcttttt tatcattagc aagtattgct ctctctgaat tacttatatt
    28381 tacatcatgc gtctctctag tcattattac attataacct ttagattcta atatattctt
    28441 taaaacaaga gatgcttcta agtttaaaac atattctggt ttttttgtag caataccctc
    28501 tgttcctgaa gatactctcg cttttttaga ggaagaacct ggagctactg gttctaaatt
    28561 gctatctcct tttccttgat gccctgggtc tatacaaatt aaaagttttt tatttttttg
    28621 aatttcattt ttattatctg acgatttgtc ctctgataac ttatctttat tacttttaga
    28681 gttagatttg tcttcttctt tatctttatt atttttgttt gaattttcac ctttatctgc
    28741 tttttcattt gtgatatttc ctgaactaat caaatttgta gtcttgtaaa aattactaac
    28801 tggatttgat gttcctataa ttatgattcc catggcaatt atacaagtaa ctaacaatct
    28861 atattttttc attttttgct cctttcttat gctagacagt tattgtttat ttttatttat
    28921 atgaatatgt atttatttta tcatatacac tatttatttt atttattttt atgaaaacta
    28981 aggtgttatt gagtcagttt tccaagcatc aaaacataat tcttgcacaa cataatatag
    29041 aagcagcaag aaaacttcaa ttctattatt aaaataattt aataagaaag aggtgtttta
    29101 tgcaacttaa aaattttcat tttaaagata atactttaag taaaaaaacc aacttagcta
    29161 agtcaaaatt tacaattgtt ttaacatttt tatttttatt atattttttc tcaaataatt
    29221 taatttatgc acaaaattta caaaaaaatg aacctgaata tttaatacca attggaaatg
    29281 tacttcaaat tgatgcagaa ttaaaaaata tcatagttag aaaccctgga gaagcatctc
    29341 catttagatt aggtgatgct atagtaaagg taaataatgc tgaggttgat ggatatagtg
    29401 attttgctaa taccttaaac gctttaccag aagaaaagca agtatctatt ttattaaata
    29461 gaggtggaca acttataaca atgaaaacaa ccaaacacat attagaaaaa attagtttta
    29521 acaatttact ttctggattt gccactttaa cgtatatcaa tccagataca tcagagtttg
    29581 gagctgtagg acatccaata agtgttggta atgcaagaaa aatacctatt aaaacaggtt
    29641 caatttctac aactaagcac cttaatatag aaaaatctta taaaggtagt gttggatgta
    29701 taaatgcaaa acgagaaaat attattggta gctttacaga aaatactgat tttggaataa
    29761 gaggtcaaat taataacttt gatatttcca agttaaaaaa atataaagta gcatctttag
    29821 atgaagttaa attaggaaaa gctcaaataa ttttacaaga taactttaat gagtgtaaaa
    29881 aatataatat tgaaattata gatatagaaa accaaaaaca tccagaaagt aaaagtttta
    29941 aaattaaaat tacagataaa aagcttttaa ctcaaactgg tggtattgtc caaggtatga
    30001 gtggtactcc aatagttcaa gataataaaa taatcggagc agtatcacat gcaattgaaa
    30061 atgacccagc tgtaggttat ggtgtattta ttaaatggat gttgtaaata atataaataa
    30121 ataagtttta agacgttatt aagttattaa taatatattt ggtctaaagg taattttatt
    30181 taacctacag accaaatata ttagatattt tacatagtat aaaatataaa ttttgactaa
    30241 aattatatta atattatttc caaaaatttt atttataaac tattcctttg aatttattta
    30301 aaaatataca actttatatt acttgttagt attttttcca gtgttaggta aaacatttcc
    30361 tagatttttt tctggaatat ttacataact ttctggaaca tcacctacta ttatagtttc
    30421 acaaataggt atttgagctt taatttgttt tgttgaagtt ataagtggta ttactacttt
    30481 aacctgtgtt tgagcttcta aatatattct atgcctagtt tgatttatac ctgaagactc
    30541 aaattccgtt ttaaagttta ctgatactgt tcctataggt tcaatagaca cttcaagttt
    30601 aggaccgtat ttagctaata taggacttcc taatgctgtt cctataggta tgtaagaact
    30661 tgctgtctta tcctttttta attcatcttg tatttctaaa gctattgcag atgcaatttc
    30721 attcatcatt atagtatttg cttgtatcat agttattttg ccattattat caagctgtat
    30781 atccattaaa tcttcataat ttatttttcc ttctaccatc tcagcaacag ctttatttat
    30841 agacctgttt gctaattcaa gagctttagt ttctgctaaa actgttattg ttggtcttaa
    30901 agttttatct atatatataa aactgcctat aaatacagac aagaaaagaa ttactaaaaa
    30961 aatagctata atttttctaa attcattttt tgtttttcta tctactattc tattcaaaat
    31021 tatcccctcc tatttattta tatgtagctt tggcataatt tttataatat ttgtcaaaaa
    31081 taaataaaat aaaaatatag actttttaat atattatgtt tatacatata caatgtacag
    31141 aatattatag caaattcatt ttttctatta attacaaaac tactcctatt tgtgttataa
    31201 tattgcatta agtaaggagg ttaaaaatct tatgaataac gataatgata aaaatggaaa
    31261 taaaatcaga agaaaaaaag ttagctcatc tggtaataca aataaaccaa ttaatagaac
    31321 ttcagctaac aaaactcgtt caacaaaaaa taagaaaaaa tctaaaaaaa gcgataaatt
    31381 taaaaaacta agagtatttg ggatagtatt tttagtttta ctagttgttg gtacagcagg
    31441 tactgctggt ctagtatttg catcactaag agatgtaact ccagttacag aagctgtttt
    31501 agataaacag acaaaccaaa caactacaat taaatacgct aatggaaaaa cattatctac
    31561 agctccaagt gtaaataaaa agacacccgt tccattagat aagatttccc cttatcttca
    31621 acatgctgta attgcaatag aagatgagcg tttttatgaa cataaaggtg tcgacattaa
    31681 aggtttattt aggtcagtat taaagaccct tacaggtact aagcaaggtg gtagtactat
    31741 tcctatgcag gtatcaaaaa tgttgttaac aactgaacaa caaactatac ctcgtaaaat
    31801 aaaagatatt tactatgcac atgagatgag taaaacagta agtaaagata aaatactaga
    31861 aacttattta aataacttct tcgttggtag aggtctagca ggtgctgagg ctggtgcacg
    31921 tggttatttt gataagtctg cagctgacct tactctagca gagtcagcac ttcttgctgg
    31981 ttcaactaaa aatccatcaa gattttcagc ctataaaact tctaagttag agggaaatga
    32041 aactaaggag gatttagaaa ataaattatt attttttgtt aacacaactg atgatgattt
    32101 agatgaccct actcaagttg attttgatat gattgaaaaa attaaatctt gggaccttat
    32161 atctagtgac acttatagac aactaaaagc aggtacatta gttgttagaa aggctgtaag
    32221 taatcctgaa gctaagaaaa gacaagaaat tgttttaaag aaaatgttag acttgaaata
    32281 tataaaacaa agtgaatatg atgaagcaat caaagctaaa atagaaataa aattgcctaa
    32341 atcatcagat aaagtttctt catctgtaga agaccttatc gaaagtgaag ttataaatgc
    32401 tttaatggaa caaggacata caaatgatga ggctcaaaac ttattctata atggtggttt
    32461 aattgtaaat acaactatag accctaaaat gcaagatgct ttagaagaag agtttgataa
    32521 aaatagcaat ttcccaggac atatggtcgg tcctgatgga gttagccaac cacaagctgc
    32581 aatggtaatc ttagattata aaaatggtga aataagagca ctagctggag gaagaaatat
    32641 aagtggtaga aaaactttaa atcgtgctac taaccctcat caaccaggtt cttctataaa
    32701 acctttagca atatatacac cagctattga tactctaaaa ataacacaat caactgctct
    32761 aagtgattct agaggaggat ataagtttga agaaaataat aaatggaatc caagaactac
    32821 tactgctggt catggctcta tgagtttacg taaagcactt gctaaatctt caaacacaat
    32881 agctgttaag actgctgaaa tgcttggaga ctcttatgat gaatgtgtag atataatgat
    32941 ggattactta aagaactttg gtataacaac tttaaaaaat aatcactcag gttcatctga
    33001 agcaagtgat agaaaattcc catcattgac actaggtggt atggcgaatg gtattactcc
    33061 gcttcaaatg gctgctgctt atggtacatt ggcaaatcaa ggtgtttatg tagagccttc
    33121 tatttttact actataacta cctttgatgg tcaattatta gttaaaaaag ctcctgaaga
    33181 acataaagtt gtagaccctg aagtagctta cgttgttaca gatatgttag aaagcgtaat
    33241 tactgaaggt actggtggtg ttgcagcact tccaaatgga atgcctgtag caggtaaaac
    33301 aggtactaca aatagtgcat atgatgcttg gtttgtcggt tacactcctt attatgttgg
    33361 tgcaacttat attggagatg atgctggtag aaaagatgat tctggtaaca ctataaaacg
    33421 tagagaagta ccacatggaa gtactagtac tgcaaaacta tgggaaaaag tcatgcaaaa
    33481 aatacatgct aatttaacag ttactgaatt tgaagttcct aaaaacgtat attttacaaa
    33541 aattaattta gatgatggag gaaagcaatc ttctggctct aaagcagcat ttattgaagg
    33601 tactgctcct actaaagtaa gttctcaacc atcatctgaa gatacaaaaa aaccagataa
    33661 taatcaacaa caagaaaata ataataacaa taataactct ggtgataatg gtggcggttc
    33721 tactcctcct gataatggtg gaaataatgg tggtggctct actactcctc ctgataatgg
    33781 tggaaacaat ggtggtggct ctactactcc tcctgataat ggcggaaaca atggtggtgg
    33841 ctctactact cctcctgata atggtggaaa tagtggtggt ggctctacta atacccctga
    33901 tggtggtgga actccagcaa cttaataaaa aataaaattt gaataaaaat ataaggagtt
    33961 gtctcaaagt gataaaaaaa atcactttga aacactcctt tttttataat taaattatat
    34021 tatgtatttt cataaaaaag agaaatctga taaagtatat tatactctat gtttgtagct
    34081 tttctttgat gtttattgac acatttatat gtctttgcta caacgataat attagttgaa
    34141 caacataaat taccaaaaga aggatagaaa aatgcaacca aaaccaattg ttacaaaaca
    34201 agatattatt aacattgcaa ttaatttggt aagtatctcg cttatgagca atatataaaa
    34261 ttagcagata tggaatttga aatgctgttc taatacataa cagaacgaaa aaacagtgca
    34321 taccattttc ctacaatacg cactgtttcg tctcaatcct gcatataaac cattaagtta
    34381 gttttgatgt tatacttatt tattggcttt tacctaaata gaactctcct tatttatgta
    34441 atcaaataaa tattttattt tttaagtctt aaactatatc ttttatttct agttgtttat
    34501 atattgtaaa tatttatatc acaaattata taaacttaac atttttctaa ctactgtata
    34561 ccaaatgtct gaaatctatt taatttacca tatcttttaa taagcatttc ccactcttct
    34621 ttatcataga aatcacactt atttctacca aatagcttgg caacttcaat tgcaaatcta
    34681 ccagctgaat ctaaatcact aaaattagtt gcacctgtag cgcatccagc aacaggcatc
    34741 tctgttgtaa ttgctacccc tactattggt gcatctgtag ctactgctgg ttgtaaaata
    34801 ctatttaagt gatacagatt gtttccatat ggtgttatat cttgaataga taaagggaat
    34861 acaaatggag acttcccagt tacagtctgc ataatatcta aaaggttatc actagttttt
    34921 aatatgtagc cctctttaac agttggtgat attgcaaatc ctctagtatt gattacccta
    34981 tttcctttag tagtatctat agaaagtatt gcatcacaac tactatcaac ttcattttca
    35041 ttcatagtca tcatatcaat tggagaatcc ataaaaggag taggatagtg ttctttagtt
    35101 ggagcatctg ggcaaatatg agttgaaatt acaacatcac catctaatat atcccctttt
    35161 cgttgcatat ccaaaagttt agcagccact gcaattgcta ccaatgctcc atcaccatct
    35221 gagacaaaac ctataacttc tggtcgtgct ccaatccctc ctagtctacc taaaatacca
    35281 agagtaggag catctccacc ttttgatttt ccatttttac caggaattgt aagctttact
    35341 aaatctgttg agcctttact tgaagacaat tcttttacag ttacatctac ttcaccataa
    35401 ccttgtaaat aagtttttac ttcttcccca ttagcatctg ctttatctaa aatgtcataa
    35461 acctctatta cttgttttaa tatcatgtct aatttgattt cctttcttaa tatattttaa
    35521 aattatatat tatttactat acaaaatatc tactccacac attgctagca ccttaattgc
    35581 ttttataagt gctactctac cttcttcccc accagctgca acagcaaatt catctgtatg
    35641 agttgcagta ttttctttta atttgatata cgcttgtatt gcaggtatct catgagttaa
    35701 atttcctgta tcagttgaac ctataccttg acttaaatct ctttcaacat agtcttcacc
    35761 caaaacttca aaattttgtc taactatatt cactaattca gaatcattct taatctcttt
    35821 gtatatttca tctaattgaa ctatttccac actagttcca gtagatattg cagctccttt
    35881 agcacaattt ttaagcattt cacaaatctc atttaaatat tctatgctta aagctcttac
    35941 attaaatata gctgtagatt tttcaggaat tatattatgt atgctacctc catcagttat
    36001 aattccatgt actcttgcat aatctttaag atgtaatctc atactattta cactattaaa
    36061 tagtgctatt actccactta atgcactttt tccttcccaa ggaaaagcag cagcatgtga
    36121 tgccttaccg ctaaaatcaa attttaaatt aacttgagca aatgatatgt catcaggcat
    36181 tgaagcatca cttggatgta aaattaatgc agcatctaca ttattaaata caccctcttt
    36241 aatcatagtt atttttccac ctactctttc ttctgcaggt gtcccaaaaa cttttacagt
    36301 cccttctata ttatgttttt tcatgatttc ttttaatatg atacctgcac caacagaact
    36361 tgtccctatg atgttgtgtc cacatgcatg tcccattcct ggaagtgcat catattcagc
    36421 taaaaaagca actgttttcc cattagttcc atttgaataa acagcttcaa aagatgtttc
    36481 taatcctcct acacctttag ttacattaaa ctcattttct tctaataaat cacataatgc
    36541 tttttgtgac ttatactcct caaatgctaa ttctggattt gaaaataaat aatcagaaac
    36601 tttttcaaat gatgtctttt tttcttcaga caataacttt atttcctctt taatttttac
    36661 ttttaattca ttcatattta ctacctcctt aaagcattcc atttaatttt aaaactgttt
    36721 gagatataaa agcagaacca aagtaagtag atgtcataac tataaatgat ataactacta
    36781 acttccaact caaagtcttt aatatatcta attggttacc tacagagatt cctgcaaaag
    36841 ctaataatgg tgttactgta gacataaaat taatttttcc tacattcgtt gctatgatat
    36901 cccccactgg agatattgga agtgataata caaatgcaac taatgtagcc catgcaaaac
    36961 ctggtaaatt aggtttttta acaaaattct ttattataat agcaattatc accaacaaaa
    37021 atccagatac acttccaatc aataaatcct gagcctgagt cttaagtgct atcatttgtc
    37081 caaagcaaat aataatatat ataaacaata ctatttttat ctgttccata atggtcttaa
    37141 aagttttttc catatatata tcctccttta caatttatta cttgttcttg ctgtttttct
    37201 tcctctaaat ctttcaataa ctggagctac tttgctataa tagtagttgg ctactggtaa
    37261 agataaaaat aattctatat atattccagt aacagctgta atcaatccac tagtcgctga
    37321 atatgctaaa atctgctctg acatttgtgg aactgtattc attaatgatg ctgttgcagc
    37381 tcccatcatt gaaccactac ccattcctga tgccattgct aaagcatatg gatgaaatcc
    37441 taaaagcaca cccactggtg ctaaaaatga aaataataat gttccaaata tactaccact
    37501 tatataagtt cctagtgttc ctatcccttc tggtgaattt aatccatact tatctccaat
    37561 aattcctagc gctgtatcac gacataatga aaaagtacca cctaatgcac ttcttccaag
    37621 acctaatgct actccaacag gtaatgctat caacattgta cccatatgac caaattcttg
    37681 taataaaagt gctggtcctg cttgaagtaa tataccaact ttaggccctg cattaattcc
    37741 aaacattatt cctaatggat atagtgttaa agacaataaa tatggagata catttatttc
    37801 attatcccct ataaattttt ttaaggctgg tataactctc cctaataaat gaggagaaac
    37861 agccattcct atgaatatag cccatagtat actaagtaaa gttacattaa ttcccataac
    37921 tttaaatgaa ataggtccaa ttaattctgc caatattgtg attataaagg ctgctattat
    37981 ggcatttcta atccctgctt tttctttcat ttctatacct ccttgtaatt tttataattt
    38041 tcttataata taattgttag tgattttatt atataaccta tcattg
//
LOCUS       DAMJVM010000027        37993 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.027, whole genome shotgun sequence.
ACCESSION   DAMJVM010000027 DAMJVM010000000
VERSION     DAMJVM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37993
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.027"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(94..633)
                     /gene="scpB"
                     /locus_tag="PC523_001506"
     CDS             complement(94..633)
                     /gene="scpB"
                     /locus_tag="PC523_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDF2475041.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIVNKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVVTNE"
     gene            complement(635..1354)
                     /locus_tag="PC523_001507"
     CDS             complement(635..1354)
                     /locus_tag="PC523_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDF2475042.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKDKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            complement(1384..1980)
                     /locus_tag="PC523_001508"
     CDS             complement(1384..1980)
                     /locus_tag="PC523_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HDF2475043.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAEYVFLYSIGFAAFNLLPIPPLDGWGVISTFVPYKWNEYMY
                     KFESMSYIILLIALFTGIYRIILTPIYAVLLKIVYLFV"
     gene            2278..3114
                     /locus_tag="PC523_001509"
     CDS             2278..3114
                     /locus_tag="PC523_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2475044.1"
                     /translation="MKKEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPEFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLH
                     SNLENTNIEVQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPINAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            3135..3557
                     /locus_tag="PC523_001510"
     CDS             3135..3557
                     /locus_tag="PC523_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HDF2475045.1"
                     /translation="METKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFEDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(3767..4930)
                     /locus_tag="PC523_001511"
     CDS             complement(3767..4930)
                     /locus_tag="PC523_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2475046.1"
                     /translation="MKKLASTALAILLAFTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGDIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELVNTKDINKASYLQMLQRIIDNMI"
     gene            complement(4984..5184)
                     /locus_tag="PC523_001512"
     CDS             complement(4984..5184)
                     /locus_tag="PC523_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HDF2475047.1"
                     /translation="MDNVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            complement(5390..7066)
                     /locus_tag="PC523_001513"
     CDS             complement(5390..7066)
                     /locus_tag="PC523_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDF2475048.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGIGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDIYKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            7283..8020
                     /locus_tag="PC523_001514"
     CDS             7283..8020
                     /locus_tag="PC523_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF2475049.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTLGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKI"
     gene            complement(8137..8595)
                     /locus_tag="PC523_001515"
     CDS             complement(8137..8595)
                     /locus_tag="PC523_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2475050.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(8877..9164)
                     /locus_tag="PC523_001516"
     CDS             complement(8877..9164)
                     /locus_tag="PC523_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDF2475051.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            complement(9242..9667)
                     /gene="ruvX"
                     /locus_tag="PC523_001517"
     CDS             complement(9242..9667)
                     /gene="ruvX"
                     /locus_tag="PC523_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDF2475052.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKEETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            complement(9819..11990)
                     /locus_tag="PC523_001518"
     CDS             complement(9819..11990)
                     /locus_tag="PC523_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475053.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGKKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYGTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILSTFGLE
                     NKEMVGRSYTLVNKEDNVNYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKGNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLYVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAILLAVSAVVVVFAFAALDIVSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            complement(12000..12257)
                     /locus_tag="PC523_001519"
     CDS             complement(12000..12257)
                     /locus_tag="PC523_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDF2475054.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            complement(12282..14921)
                     /gene="alaS"
                     /locus_tag="PC523_001520"
     CDS             complement(12282..14921)
                     /gene="alaS"
                     /locus_tag="PC523_001520"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDF2475055.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVKLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAKEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGSISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     misc_binding    complement(14989..15227)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(15370..16449)
                     /gene="mnmA"
                     /locus_tag="PC523_001521"
     CDS             complement(15370..16449)
                     /gene="mnmA"
                     /locus_tag="PC523_001521"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDF2475056.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDDVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNEN
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGKDTIKIVFDEAQRA
                     ITKGQSVVMYDEDIVVGGGIIEKSL"
     gene            complement(16733..17173)
                     /gene="nifU"
                     /locus_tag="PC523_001522"
     CDS             complement(16733..17173)
                     /gene="nifU"
                     /locus_tag="PC523_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HDF2475057.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAKKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            complement(17227..18420)
                     /gene="nifS"
                     /locus_tag="PC523_001523"
     CDS             complement(17227..18420)
                     /gene="nifS"
                     /locus_tag="PC523_001523"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HDF2475058.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            complement(18438..18884)
                     /locus_tag="PC523_001524"
     CDS             complement(18438..18884)
                     /locus_tag="PC523_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDF2475059.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            complement(18993..19763)
                     /locus_tag="PC523_001525"
     CDS             complement(18993..19763)
                     /locus_tag="PC523_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2475060.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADIDELGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRARGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            complement(19794..21059)
                     /locus_tag="PC523_001526"
     CDS             complement(19794..21059)
                     /locus_tag="PC523_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDF2475061.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKNFYKINATNSSLEDIKNVISELGSINNISGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKIDIEKGL
                     KRAVEVLNKDSYMDIECNKDAIEYIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDIIKASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSVYYIPQENKTEKNIKKYLEYLDGDF"
     gene            complement(21175..21960)
                     /gene="codY"
                     /locus_tag="PC523_001527"
     CDS             complement(21175..21960)
                     /gene="codY"
                     /locus_tag="PC523_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDF2475062.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDAQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            complement(22125..24212)
                     /gene="topA"
                     /locus_tag="PC523_001528"
     CDS             complement(22125..24212)
                     /gene="topA"
                     /locus_tag="PC523_001528"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDF2475063.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELANEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDAKKVQDAHEAIRPTSVDRTPDSIKASLSKDQYKLYNLIWRRFVASQMEDSIF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            complement(24245..25345)
                     /gene="dprA"
                     /locus_tag="PC523_001529"
     CDS             complement(24245..25345)
                     /gene="dprA"
                     /locus_tag="PC523_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDF2475064.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICQYDKEYPENLKNIYNAPKLLF
                     YKGDIDLVNNKFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            complement(25364..26965)
                     /locus_tag="PC523_001530"
     CDS             complement(25364..26965)
                     /locus_tag="PC523_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDF2475065.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDISNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQKIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLNDKSKKENEELEKDNSYIDFKYTKINDEESKYNEDF
                     EDIKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISIEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLIAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFR"
     gene            complement(26996..27340)
                     /locus_tag="PC523_001531"
     CDS             complement(26996..27340)
                     /locus_tag="PC523_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDF2475066.1"
                     /translation="MNNKEKGDFGEEIAVNYLLSKGARILEKNYRLKIGEIDIIAKMG
                     REVIFVEVKSRSNIKFGYPCESVSYKKRKKIIGIANYYIIKNNLNNTPVRFDVIEVYL
                     LEKRINHIVNAF"
     gene            27524..28687
                     /locus_tag="PC523_001532"
     CDS             27524..28687
                     /locus_tag="PC523_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2475067.1"
                     /translation="MNRKKLVAFIRYLSLFILVVSYLKNVENTLYPVIIMMILFIIII
                     NNQVRFFSLSNENKFILISYFLELILIFILCNYTKTFNSIYFVPLILDISFLIKEKYK
                     YILFTLVIISSLIISLDKNIYLALESSSVLLIITILSIYIENENLSKLYSQNLYDKLR
                     ISKDELKKVNADLETYASSIEELAILKERNRISREIHDSVGHSLSTTIIQLNAIEKLL
                     KDKPLISELVHELREFVTESFQDVRRAISELKPVEYENYQSLFKIKELVKSFIKLTNI
                     NVKLTISKNTWNLSRNQSIGLYRLIQESLSNSSRHGKATEIKIFMTFNPSNFIITISD
                     NGIGCGNIKKGNGLNSISERIYELNGKVEFDNSNNGFTIRASFPKFSGGDFFE"
     gene            28680..29327
                     /locus_tag="PC523_001533"
     CDS             28680..29327
                     /locus_tag="PC523_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2475068.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKTNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTPEKMIEQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISKELFLSEGTIKNNITNILGKLELRDRTQLAIFAFKNKIVIE"
     gene            29456..30391
                     /locus_tag="PC523_001534"
     CDS             29456..30391
                     /locus_tag="PC523_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2475069.1"
                     /translation="MDIVRVNNITKRFNDKLALDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGNLLKERINEALEVTGLTEVKKKKVKKLSDGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDMSKKYETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NAYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISILENNSLLELDIDKKLQLTEI
                     LSVIDKNNSKIIRISYEEPNLEDVFLNLTGKNLRD"
     gene            30405..31505
                     /locus_tag="PC523_001535"
     CDS             30405..31505
                     /locus_tag="PC523_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2475070.1"
                     /translation="MKLYMSVKTMLKGLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNHLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIIGNNVKQSSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRIIGITFKGNFAILLLLCALSSYFMTSISTFVNS
                     FFSKKYGTIIVYALLFLQTIFGGIFSMISDAFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGNLIFTCLITSTILIALAIVKEKYKWREVQ"
     gene            31505..32569
                     /locus_tag="PC523_001536"
     CDS             31505..32569
                     /locus_tag="PC523_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2475071.1"
                     /translation="MIRLTVANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDNLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKMDKNIDDKLTKTNIIDN
                     KLDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFTLLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWIARVFKNESSIS
                     LISTFYSLIGLAISLSSLIPSMDKLSFMANLSKLTPFYWTIDAIKNNGSILINIIILI
                     LIGTIFVTAGSLKLRDFAKN"
     gene            32813..33310
                     /locus_tag="PC523_001537"
     CDS             32813..33310
                     /locus_tag="PC523_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HDF2475072.1"
                     /translation="MYKIGESVMYPKEGACCVNDIVTKKINHQMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDKFIQSIDKTDGVWVFDRKQRLKLYHDKFHSGDVF
                     EIVKLIKMLMIQDCSKQLCSTDKEFLNKAQRFALSELAAAQCKSYTVVLEEMKKHILN
                     SKSKN"
     gene            complement(33483..33731)
                     /locus_tag="PC523_001538"
     CDS             complement(33483..33731)
                     /locus_tag="PC523_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475073.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(33798..34814)
                     /locus_tag="PC523_001539"
     CDS             complement(33798..34814)
                     /locus_tag="PC523_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2475074.1"
                     /translation="MEYNELLKNARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVEQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(34872..35639)
                     /locus_tag="PC523_001540"
     CDS             complement(34872..35639)
                     /locus_tag="PC523_001540"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDF2475075.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYNKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDIAQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(35707..37896)
                     /locus_tag="PC523_001541"
     CDS             complement(35707..37896)
                     /locus_tag="PC523_001541"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HDF2475076.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRDLAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEEFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNLMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKENKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT        12153 a         6553 c         4104 g        15183 t
ORIGIN      
        1 atcattttat atttatacaa aaaacacaac aacttagaga attttaaatc tttcaaagtt
       61 aaaaattctc taagttgttg tgtttttata tttttattca tttgttacca cttcttgata
      121 attttctata ggaggcaact cttcaagact ttctatattc aacaacttta aaaattcatc
      181 tgttgtttta taaattattg gtttccctat cttattcagt ctaccagctt ctcttataag
      241 ttcattttca aacaaagttt gtaatacctt atcacatttt actcctctta tatcctctat
      301 ttctaccttt gttattggtt gtttgtaggc aattatagtt agagtttcta atgtagcttg
      361 gctcaaactt ttctttttct ttggttcaat aatcttcttt atatattctg catagtcttt
      421 gtttgtacac atttggtact tattctctaa cttgattatt tgaatacctc tattttgttc
      481 tctgtactcc tcaataagta aatttaacat aatctctatt tcttttgatg aaagttcttt
      541 atttactata tagtttaatt cttttatact tatcggctct ccatatgcaa acataacaga
      601 ttctataata tactttatat cttctctttt cattttaaca ctccgaactt tttcttatta
      661 gaatatcatc aaaaaatata tcttgtacta caactatttc tttttctttt ataagctcta
      721 atatagataa aaaagtagct attatttcat cattttcata tgattttata atatttgtaa
      781 aagtaacttc aatcttatct tttataatat ctcttatata caatatcttt tcttctacag
      841 atattatctt cttccttact attttgtcta gtttctcatc attagcattt tctatctgtg
      901 atgttttaac tttaaatatg tatgggagta tattttttat tgcttctatt gatatatctt
      961 ctaaatctac attgtcatca ataattatct cttctttatt tctataaaat ctctcattaa
     1021 cataagttat attttctttt atatcttgtg atgctacctt aaactttcta tattcttcta
     1081 gcttttccat aagttctatt cttgggtctt cttcttcatc tttttgctta taaagtagat
     1141 atttagactt tatttctaaa agttttgatg ccatagttat aaactcacta gttatctcta
     1201 aatccatttt atctagcatt ttcaaataat ttagatactg cttagttata tctattatag
     1261 atatatcctt tatgtctatt ttatgctttg ttattaaatc atacagtaaa tctaatggcc
     1321 cttcatagac ctgtaattgt atattatact tcatatcaat ccctctttaa tctgtttatt
     1381 atattaaaca aataaatata ctatttttag tagtacagca tatattggag ttaatattat
     1441 tctatatata ccagtaaata aagcaattaa taaaattata taactcatac tttcaaattt
     1501 gtacatatat tcattccatt tatacggtac aaatgtagat ataactcccc atccatcaag
     1561 tggtggtatt ggaagcaaat taaaagctgc aaatccaata ctatataaaa acacatattc
     1621 agctattgta tttatcgcat acatatgcac atatttattg ataattacac aaagtatggc
     1681 agtcaaaaca ttacaaaaaa ctcctgcaag tgaaactatc aggtttccta tcttataatt
     1741 tttaaagtta tttggattta ctggtactgg ttttgcccat cctattttaa gaataaacat
     1801 agccaaaaga cctattgggt caacatgtct tacaggattt aatgtcattc taccataagc
     1861 tttagctgta tcatctccca ataagtgagc tgaataagca tgaccaaact catgtattga
     1921 tatcgccata gctataccaa ctaaacttgc tactatttct cctatattca tacttaacat
     1981 taatttacat ctcctaaatc aaatttttta atattattat aacaattaaa taacttataa
     2041 tcaaaattat tttatattat aataatttta acttctaggc ataaaattaa aatttgcttt
     2101 ttattttata acttttatta ttatatcata ttcgttgtga aaatataata attacactac
     2161 tgtaagtgta atattatatt gacatgaatt gacattatta tgttttagtc aatatcatac
     2221 aagaagtata aaaaaatatg tgttacactt tttataacat gaaaggaggt taactaagtg
     2281 aaaaaagaag aacgcacagt atgctttgat agggaattaa aaattgaagc atatagattt
     2341 aaaggcataa tgcaaaaatt cccaaaccat tttcatgaac attatgtaat tggttttata
     2401 gaaaaaggtc aaagatattt atcttgtaaa aataaagagt ataccacaag tactggagat
     2461 ttgctacttt ttaacccttt tgatagtcat acttgtgaac aaatagatga taaagtactt
     2521 gattacagat gtatcaatat taagccagaa ataatgaaaa aaactgtctt tgaaattaca
     2581 gggaaaaatt atttaccaga atttaatcaa cctgttatat ttcgcagtga gctagtacct
     2641 ctactacaag aattacacta tataatcatg gaggaagaat tggactttaa aaaggaagag
     2701 ctttttttct ttctaattga acaattaatc gaagaacata cagagccaaa tttgcattct
     2761 aacttggaaa atacaaatat agaagttcaa gcagtatgtg attatttaga aaataattat
     2821 gctgaacata tagtacttga tgaattaagc actatagcag gtatgaataa gtactctcta
     2881 cttcgtaatt ttacaaaatt aaaaggtatt acaccatata gatacctaga aaatatacga
     2941 gtaaacaaag ctaaaaagct tttagaaaaa ggtgtagagc ctataaatgc tgcgatacaa
     3001 acaggatttg tagaccaaag tcactttaca aactttttta agaattttat tggtcttaca
     3061 ccaaagcagt atcaaaatat atttataaat gacgattcaa ataactttaa ataaggtaaa
     3121 aaggagaaat aaaaatggaa actaaaaatt caaaatgtgt catggtaata gatgaaaatt
     3181 taccaatggg aattatatct aatacagcag ctataatggg aattacatta ggaaaacatg
     3241 ctccagaaac tgttggacca gatgtaattg acaaaacagg taatagtcac ttaggaatta
     3301 ttgacattcc tgtacctatt ttaaaaggaa acaaagaaat aataaaagat ttacgtaaaa
     3361 aattatatac tttggaattt gaagatttaa ctgttgtaga tttttcagat gtagcacaaa
     3421 gttgtaatct ttatgaagaa tttacacaaa agattgctag tgttccagaa gatgagttac
     3481 agtattttgg tatagcaatt tatggaaata aaaagaaagt aaataaactt actggtagta
     3541 tgcctttact tcgataagtg ttaatcattc atgtatatga tattaaatca aatgatattg
     3601 tatgatacat aaacttttct atatttatat aaagtcataa gattataaaa tacttatata
     3661 cagtaaataa aaatacccct atggaaaata attacataag ggtatttttt atgtgtatta
     3721 taattacatc tttgtatatt ataattatat ttcacttgat aattatttat atcatgttat
     3781 caattattct ttgaagcatt tgtagatagc ttgccttatt tatatcctta gtatttacca
     3841 attccacttc gcctattact tttttatctt tacatatctt tatctttcct aaaacagttc
     3901 cctttttaat tggcatctta ggatttttta ctatttcaat tttcttctca aattctttag
     3961 agcttccttt tttaataaga gcatttaaat cttctttagc aaccaattta actttgtttt
     4021 catcagcctt atccaatgta agtgttgcta tattatcgcc tttactacat aacttaacac
     4081 tttcataatt agcaaatcca tagtttaaaa gactagatgc atcattaaat ctttctggag
     4141 atgtctctgc tcctaaagtt gcagcaacta gatgagtatt acctctcttt gcagatgctg
     4201 ataaacagta ttttgcttct tgagtaaaac cagttttaac accagtagct ccttggtagt
     4261 gttttataag tttattggta tttgcaagac ctactgtaac ttgtttcttc cctactacaa
     4321 ccttatccat ccatgtagtt aagtatttac ttatcatttc atgttttaac aattctctag
     4381 acattagtgc tatatcatga gcagatgtat agtgattagc aactggaagt ccatttgtat
     4441 ttgcaaaatg agtatcattc atatttaact cttttgcctt agcattcatt ctatcaacaa
     4501 acccttcaac acttccagct aagtgttcag ccatagcaac acaagcatca tttgctgaag
     4561 caacagctat accctttaat aacgtatcaa ccttttgagt ttctcctggt tctagaaata
     4621 tctgactacc acccatacta gacgcagttt cacttacttg aacttcatca tcaagcttaa
     4681 tatcacctga atctaaagct tctacaataa gtaacattgt cattaccttt gtaacactgg
     4741 caggaggaag ctttttatgt gcatcctttt catataaaat ttgtccacta ccaacatcca
     4801 tcaaaattgc tgattttgaa gatatattta gttgattggc atcagcattt tccttattat
     4861 ttgcaaaaga aaaactcaaa ggagtaaatg caagtaatat agccaaagca gtactagcta
     4921 attttttcat cgatgtcacc tcttaaagtt tatttaaact tattttctca catttaactt
     4981 tttttattct aaatgcctag atataccttg acctaaattt tttttctttt caagcttttt
     5041 aaagtaacct tctaaaacac ctttattaga tgcattttct attatttcaa aagcatcttc
     5101 ataattcatt ccatactctc ctgctatttc catttttgtt tgttttaaag cttttatagc
     5161 attttgtctt gaaacattat ccataagaat cctcctatca gtaaaattta tatataagta
     5221 gtttttacta ataaaagatt gttataattg gtattttatg taatatacat attttctaaa
     5281 tcaaaaacta aaaagacctc ttgtaattct taagataata tcaattggat ttttgatatt
     5341 actttaaaaa ttacaagagg tctaattttc aaatttatag ttttttatat tatacttcca
     5401 ttattatagg aagtatcatt ggatttcgtt tagttttttg atataaatat tcttttaggt
     5461 tttctttaat attattcttt aaataagccc attctttaat atttttctct tcacattcat
     5521 ttagaacatt cttaattata tccttcgcac catccatcaa atcttctgac tctcttacat
     5581 atacaaatcc tctagaaatt atatctggac ctgctaaaac tttaccctca tccttagaaa
     5641 tagtaactac aactatcata agaccatctt ctgataaatg ctttctatct cttaaaacta
     5701 tattacctac atcgcctact cctagaccat caactaatat atttcctgtt tgtatacggt
     5761 ttgctacctt agcagaattt ttatctaatt ctaaaacatc tcctgtttgc atgacaaaaa
     5821 tattttcttt gtccatacca agctgttcag ctatttcagc atgtcttttt aacattctat
     5881 attcaccatg agctggcata aaaaactttg gtctaactaa tgcatgtata agttttaatt
     5941 cttcttgaca ggcatgtcca gaaacgtgta tatcagctat atcactgtat actacttctg
     6001 ctcctttttc aaataagaaa ttgattactc ttgatattaa tttttcattt cctggtattg
     6061 gatgagcaga tattataact aaatcgccac tctttatttc tacttttttg tgctctgaaa
     6121 aagccattct agcaagagct gacataggtt ccccttgtga accagttgtt attatcacta
     6181 attcactatc ttcatactta tatatatcat ttaaatctat aagcatgtca tcagaaatat
     6241 ctagatatcc taattctttt gcaactccaa ctacattgac cattgagcga cctgatatag
     6301 caacttttct attaaacttc tcagcagtat ttattatttg ttgtaatctg tgtatgttgg
     6361 atgcaaaagt agcaactata attcttctgc catttccttt tgcaaataat tcgtcaagac
     6421 ctatcccaac agtcttttca gacatagtaa atcctggttt ttctgcatta gtactatcag
     6481 ccagcattaa aagaactcct tttttgctca actcacagat tctatgcata tccataacat
     6541 ctccatcaat aggagtcaag tcaatcttaa aatctcctgt atggtatatt ataccttggt
     6601 ctgtgtgtat tgcaatagag taagcatctg gtatactatg gttatttttc aaaaactcta
     6661 cttccatatt atcaagcttt ataacttgtc taggacctat aacatttaac ttaacattat
     6721 tcatcttatg ttctttaagt tttacttgta taagacctat actaagtctt gctccataca
     6781 caggtacatt tattttcttt aatatatatg gaagagctcc tatatggtct tcatgtccat
     6841 gtgtaataaa tatacccttt attttatccc tattttttac taaataagtt atatctggta
     6901 taactatatc tactcctaac atttcatcct ctggaaaact aagtccagca tctataacaa
     6961 ttatttcatc tttatactca acaacagtca tatttttccc aacttcgtta agtccaccca
     7021 aggccattac ctttatcttg ttggtgttct ttttgaacaa ttgcatcatc tccttctgtc
     7081 tatttaattt tttaacacgt tctacaatca taaataatat caaatcacgc actcgctacc
     7141 agttattctt attgtaccat aattcgtatc atattttaag ctatatttaa aatacattta
     7201 acgtattatt ttgacaaacc tattttattt tgatatcctc taaatattat atgatttaat
     7261 atcggataat ttaggaggat ttatgcgtgg taaaactcat tgtgcaatag gaatcttaac
     7321 agctatccag acatctttaa tatttaaaat tcctatatct ttagttgata ttttagtttc
     7381 agctactttt gctgtactgc ccgatttgga taaatctaat tctatggtct caaatttcat
     7441 tctaaagaac aatgtatcca aatacatata tagaattttt atatatgctg ttaatattat
     7501 tattttcttt atttcaataa atataaatga taatttttat ttaagcgcaa taattacatt
     7561 tgttgcaatt ataattattg aagcaaaact aacccacaca tttctaagaa aagtttttct
     7621 atcattaata tttatactat tagctatatg cttatacatc atagaagttg aaatatattt
     7681 tacaattttt tgtctaatgt tgtcaatttt tccatggtta aagcacagaa gtttttctca
     7741 tagcatattt gcaactataa tagtatattt tttactaaaa cagatagaat taatcactaa
     7801 tattaataat ttatcttttt atggtacgct tggttatgca agtcatatgt ttttaggaga
     7861 tttatttaca aagcaaggaa taccaatttt ttatccacta agcgaaaaga aaatttcttt
     7921 aggattttta actgttggag gaccatttag taatttcata gaaaaatcat tcatttttgt
     7981 acttatagga ttaattatat tttcaatatt aaaaatataa ttagtttaaa aactatatat
     8041 ttacacttta tagattgctt ataataaaaa atggaagaat agcatagaat ataaaagttc
     8101 tatactattc ttccattgac attaagcaat agaagtttat tcttctgagt cgcttactcc
     8161 attacactcg tcacataatc cataaaattt aacatcatga tcaagtattt taaatttgta
     8221 caatgattgt atctcttttt ctatattgtc taaaaggtct tctttagctt ccattatctt
     8281 tccacaattt ttacaaataa gatgatgatg attatgacag tcttctttat gtagactaat
     8341 ctcatatctt atacatccat cgtctaagtt tagttttgat attaacccta tttcatccaa
     8401 tagttgcatt gttctatata cagtagctaa tcctatttca ggacagtcaa ccctaactaa
     8461 atcatagatt tcctcactac ttagatgtga atggtcatgt ttaagcaata tttcaactat
     8521 tgctcttctt tgtggcgtaa tcttaaaacc agtttctttt aacttgtctt ttaataaatc
     8581 cattgtattt gccataacat cacccttact attttcaatt gataatcttt attaaataat
     8641 attgtaattg ataattcatt ttttgtcaag acatttaaat aaatttaatt tattctcata
     8701 atataaatat gtacccataa aagatatatt attcaccaaa attcataaat ttcttgatat
     8761 ttttaataaa aaacaaaaaa acagatatat actataagtg cttttacaat taataaggtt
     8821 taattaaagc aaatataata aatacctgat ttttatatac aaacttaatt atttatctag
     8881 ttaagtccat ttgattcatc ttcgtcgtag tttaaaccct cttcttccat tatagcagta
     8941 tatacagcta caacagtttc atactcttca tcactttcta aaggtattaa gatttcacct
     9001 tcttcatcct catcaattct aaagattaaa gcttcttcag cttcttcatc atctaaaggc
     9061 attaatattg catactcttt tccttctgct tctaatgtta atattatttc aaattggctt
     9121 tcaacgccat tttcgtctat taaatttatt atattttctt ccataatgtc attcacctca
     9181 aatttatttt tatttttatt atctatatta tatatcataa gtattcaatt ttatagttct
     9241 tttatgaatt tattttaaaa tctaaatatc cttgcaaaat tattactgct gccaacatat
     9301 ctataacctt ttttcttttt tgtctactga catctgcctc taataaagac ctctctgctg
     9361 ctactgtgga aagtctttca tcccaaaatt ctacatcaat accagtttct tcttttacca
     9421 actcacaaaa cttgattact ttttctcctt gtgggcctaa agttccattc atattttttg
     9481 gcaatcctga tacaatttta tttacctgct tttcttttat tattgctttt agctcttcaa
     9541 tatctttttt cttaccaacc ctttttattg tttttacacc ttgtgcagtt agtcccatca
     9601 aatcactcac agcaactcca atagttttat caccaacatc aagacccatt attcttccat
     9661 ctagcataat tcctccttta tctatttata ttccatagat taaatacata ttatcaatca
     9721 taaaaattgc aatatgattt tctatgtaag catacagttt acagtaataa tcatattgca
     9781 ttttttattt aaattctata tgaacagtaa aaaatttttt agttattttt ttcaaatatt
     9841 atcatattta tacttattat tattagtggt ataagtatat atatagatgc tgtagatgca
     9901 gcaactattc cggaatcatt aactaaaaga gtgattatac aaccaaccat agatgcaata
     9961 aaccctttaa acaagattgg aaaattattc attatctttc taaagtgtct agatggttta
    10021 aatataagta ctgcaattac tccaattcct acaagtaaaa tatttaccca tacacttgtc
    10081 tgtgctagtt gcatattcat ttgaattttt cttccaaatg tttgtaatat ctctcctggt
    10141 ccatttagga aaattctttg tacaaacata cctaaatgag attcactacc tgaaactata
    10201 tctaaagctg caaatgcaaa aacaacaact acagctgaaa cagctaataa tatagctttc
    10261 ttaaaatcta ttttaatgtc atatataagc attataaaca acaaatatgc tacacattct
    10321 gatatagctc caccaacatt tgcacccatt gaaggatatg cactagttat aagtattatt
    10381 aacgaaaaaa ttacaacata caatttagat accttcttat aatgtaataa tactgctaag
    10441 ccaaatactg cacttgctat agttacaccc tcatattcat ttcctacacc atagtaccta
    10501 gctcctatga cagcatcata actcattatg ttattttgca tcaagtatgt tccaaataca
    10561 gaatctataa ctatgactaa aatagtaagt gttgcaaaaa atcccatgtg ttttatatca
    10621 ttgcccttaa acaattttct acctaacaag tataatatta gcgtagtcgc aaatattccc
    10681 aaagatattg aaattggagt tttaaaattc atcaaaggag ctaacataaa tgatagtggc
    10741 attattatgc ctaattttat aaactcctta agtactttga atacacgctc tttatgtggc
    10801 atataatttc taaagaaaat cgcaaacatt gcaattaccc aagaagcaga tattacacct
    10861 acaaagccat ttataatagt tgaccttatt gaagatatag acactatcct ttcataaagt
    10921 gattttagat aattaacgtt gtcttcttta tttactaaag tataacttct acctaccatc
    10981 tctttatttt caagaccaaa tgtacttagt atatccactc caacatctaa gttggcaaca
    11041 attccagcct tccttgttgt ggcagattcc agtagacctt tcccttctcc actaaatttt
    11101 attattggag aaagtcttct cttattctta taatctaggt cacttggaaa tgcacttgct
    11161 atataaacag tgtcattttc tccaaccata gaaaatactt gttcaagata tttatttata
    11221 ttttttacaa tattcttctt cattgtacca taagtattat catttagatt taatctatac
    11281 atatctaatc tatatgtatc tccaagttct acaaatatag catcactatt tttataggat
    11341 ttcttagttt catctagtaa tttatcgtag tctgtagata ttccaaatgg cattttagaa
    11401 tctttcttat taatattatc aatattacct gaatcaattc ttccatattg gtccatagct
    11461 actagacata aatttctatt cttaactaat tgattatttt caattatatc agcatttcca
    11521 attacagatg tctttaaacc attttctgat aaagtttttc ctaatgaccc taaatttgct
    11581 ccatactctc catactcaag attttcattt atagatctat taatacctaa atcatttatt
    11641 tctttagctt ttttacctgt tgcgacttca aaagctttag cagtatcttg gtctactttt
    11701 tgaaaattga tatagtcttc agtagttaca ctagctcttg ttcctgcacc catacttgca
    11761 taagaccttc tatcatcagt accttgatcc cctcttatat tcatgagagc tacatatcct
    11821 ctcttttcta tctcattttt caagattgga atattcatca tacttccaag attggtccta
    11881 ttcatatcaa taaatatgac cttgcctttt tcttcggcaa aaccatagct agcagttgat
    11941 acaaaaatca taatgactat tgcaaacaac gacataatct tttttctcat ttatttgacc
    12001 tactttctat tcagatagtg cgtgattact tcttcaagta tctcatctct ttcaaatttt
    12061 ttaattatag ctcttgcatt tttatgacta gttatatagc tagaatcacc agaaaggaga
    12121 taccctataa tttgatttat agggttatat cctttttcaa ctagtgcttt atatacaaaa
    12181 tcaatagtat ctcctacact catcctatct tctggtattc cctcaaattt catagtgtag
    12241 tctaaatctt tttccaaact aaaaacctcc ttgagtgaat gttatttaac ttgtgtctta
    12301 ataacctcac ttgcatagct taaagcttgg tcaaccttag atacatcact agctcctgct
    12361 tgtgccatat taggtcttcc tccacctttt cctccagcta tttttgcaac ttctctaaca
    12421 atattacctg aatgtattcc cttatctaat acatctttag tggcagtaac tacaaagtta
    12481 actttatcat ctactacatt tgcaagtact actacaccag aaccaagctt atctcttaga
    12541 ttatctgctg tttctcttag tgtatccata tccatacctt caaatttatt agttatcaaa
    12601 ttaactccat ttacttccac tttagaatca aaaatagaat caactgactg taaactcatc
    12661 ttagctttca tatcatgcaa ttctttagat aagtttttat tttcttctaa taaagaactt
    12721 attctttgaa ttaaattatc ttctttagat tttaagttta cacaaacttc agagataatt
    12781 ccatctcttt cttttaggta ttcataaaca gcttttcctg ttattgcttc tattcttctt
    12841 acacctgctg ctactccacc ttcagataat attttaaaca ttcctacttg agaagtattt
    12901 gtcaagtgag ttcctccaca aagttctgta gaataatctc ccatagaaac tactcttacc
    12961 tcatcaccat atttttcacc aaatagtgct gtagctcctt tttctttagc ttcctttata
    13021 ttcataatat cacattttat gtctaaagat gataaaataa cattatttac tttttcttct
    13081 attactttta attcttcatt tgatattgct tcaaagtgag taatatcaaa tctaagtctt
    13141 tctggagtta ctaaagaacc tgcttgatta acatgttctc ctaacacttc tcttaatgct
    13201 ttatgaagta aatgtgtagc actgtgattt cttgcagctg acattctagt ttctctatct
    13261 actagagtct ttactttatc tccattactt atgctaccag attttattat acctatatgt
    13321 tttatactat tattagcacc ttttttagta tttaatactt ctaaaactaa gttttcatta
    13381 gttatcaatc cacaatctcc aacttgtcct ccaccttcag gatagaaagt agtgttatct
    13441 agtactatag ataccttatc gccttctttt gcactttgaa ctagctcatc atctttaact
    13501 atagcttcaa tagttccttc accataaatt tcactatatc cattaaatgt actatcaact
    13561 gtagattcta attttgatag agggtcttcc ttccaacttt ctccatccat atttcctcta
    13621 gcatttctag ctctttcttt ttgcttttcc atttcttcat taaaagcttc ttcatctaca
    13681 gatagatgtt cttcttctaa tatttcttta gttaggtcta tagggaatcc ataagtatca
    13741 tatagtttaa aagcttcttg accacttagg acagtctttc cttcattttt taatacttct
    13801 atataagagt tcaatatttc tgttccttgg tctatagttt cattgaattt ttcttcttct
    13861 attccaacta cttttttaat atagctttct ttttcaacta aaactggata tgctttatca
    13921 tttactttta taacttcatc tattagtttc tgtaaaaata attcttttac tcctaataac
    13981 ttaccatgac gagcagcacg tctaagaagt cttctaagaa catatcctct tccttcattt
    14041 gatggaagca ctccatcact aactaagaat gttacggctc ttatatggtc agttattatt
    14101 cttattgata tatcattttt tggattctct ccatatttta ctcctgttaa cttctcaact
    14161 gcttctagta tagatttaat tgtatctact tcaaagatag tatctactcc ttgcattata
    14221 catcccattc tttcaagacc catacctgta tctatattct tattttctaa taaactataa
    14281 tttccttctt catctctatc aaattgagtg aatacatggt tccaaaattc taaatatcta
    14341 tcacaatcac agcctggttt acagtctgga ttgtcacatc catattcttc tcctctatca
    14401 aaatatattt ctgaacaagg accacaagga cctgtaccta tctcccagaa attatcatct
    14461 ttcccaagtt taaccattct ctcttctggg aaattcattt cttttgccca tatatcataa
    14521 gaatcatcat cttcttcata tacagtcacc catatcttat cttctggtat atttaaccat
    14581 tctgtaacaa attcccatcc ccatttaatt gactctcttt taaagtaatc tccaaatgag
    14641 aagttaccca tcatttcaaa gaatgtagca tgtcttgcag ttatacctac attttctata
    14701 tctcctgttc ttatacattt ttgagatgta cacattctca cacttggagg tacttctaca
    14761 cctgaaaagt aatttttcaa aggtgccata cctgagttta taagtaacaa actcttatca
    14821 ttatttggta ctaaagaata actatttgct acataatgac cttttgactc aaaaaattct
    14881 aaaaacttac ttcttatttc atttaatccc atcttttcca tatcatgttc ctcctaataa
    14941 attttatttt ttcgtttaaa taaaaaacct cgtccctata aataaattta taggggcgag
    15001 gacaatcgcg gtaccaccct aattatcata caaagtatga tatcttcgga aaaccttagc
    15061 tataacgtaa ctaccgtcaa tctctactta aatattcttt ataaaagata aattgttcaa
    15121 gattgctact aaaaggctgc ttcaaatatc atattctaag ggcttccagc atttccctct
    15181 ctctgtaaga catcaaatat tttactattc cttatcactg tatttgtttt ttaattaata
    15241 tactaattat attactaaat tttacaaaat taatcaatac tatattttca tatacaataa
    15301 atttatttta taaactttat ttactcttaa tataccttat tgtttatttt tttaattttt
    15361 tagatgtttt tataaacttt tctcgattat acctccacct actacaatat cttcatcata
    15421 cattactaca gattgacctt tagtaatagc tctttgagct tcatcaaaaa ctattttaat
    15481 ggtatctttt cctactctat gaatagtagc tggtgatggc tttgctgaat atcttatttt
    15541 ggcttggact cttaatggct cttctaacgt atctattgat ataaagttaa catctttggc
    15601 aataagttcc tttttaaata aattttcatt atctccaaga actatggtat tgtttattgg
    15661 atttatgtct attacaaaca ttggttttcc aaaagtaatt ccaagccctt ttctttgacc
    15721 tatagtgtaa tataatatcc ccttatgctt tccaagtata ttgcctttag tatctacaaa
    15781 aaaaccttct tcaatacgct ttttagaatg tttttttaca taatttgcat agtcattatc
    15841 tttgacaaaa caaatttctt gactatcagg cttattgtgt actgccatac ccatttcttt
    15901 agcaatttct cttactctat ctttttcata gtcacctatt ggcaataaag tatgctctag
    15961 ttgctcttgt gtaaggttat aaagtgcata agtttggtct tttttatctg taacagattt
    16021 ttttaacaaa tatcttcctg tactttcatc cttttctatc tttgcatagt gacctgtagc
    16081 tacataatca caacctattt gtctagcctt cttataaaaa tcatcaaatt ttatatgttt
    16141 attacaagct atacaaggat taggagtttt tccttccaaa tattcatcta taaaataatc
    16201 tattactttt tctttaaaga cttctctaaa atttaaaaca taaaatggta tacctatctt
    16261 atttgctacc cttctagcat cttcaacagc agataaagag caacaaccac cttcaatttc
    16321 aacatcgtca tcatcttgcc acaacttcat agtaacacca atgacatcat atccttgttg
    16381 ttttaaaaga tatgcagcta cagagctatc aactcctcca ctcataccta tcattacttt
    16441 tttattcata aacaaatttc acctcatctt ttgcattact tattattata tcatatagaa
    16501 caaacattgt atttatttaa aatatagctt gctctatttt ataaaaaaaa taattatttt
    16561 cattatctaa atattataga aatatatttt gttaattaat tgacttctaa aacaagtaaa
    16621 aactattaat ttaagcaata aaaaatagct attttacaaa cagctatttt ttatatctca
    16681 ttaatacttc tttttagtca ttttaaactt ctcataacac aattacttat ttttattctt
    16741 cttcttcaac atcatggtca tgagcatcat ctataacata cccatccaat tctggtatat
    16801 ggatattgtt ctttttagca tagtctataa gagctgcttt tacagcttgt tctgctaaaa
    16861 ctgaacaatg catctttact ggtggtaaac catccaaagc ttctgcaaca gctttgtttg
    16921 taagttctaa tgcatctttt atagtttttc cttttatcat ttcagttgcc atagaagaac
    16981 ttgctatagc tgaaccacag ccaaatgttt taaacttaac atcctttatt acatctccat
    17041 ctatgtctaa aaatatcttc attatatctc cacatgtagg gtttccaact tcacctacac
    17101 cacttgcatt atcaatttct cccatatttc ttggattcat aaaatgttcc ataactttat
    17161 cactatattg cattatcttt tcctcctact caatttatgt ctatttttta tgctttatct
    17221 ttctgtctat tttactaaac cttgtttctt agcatcatca taaagtggtg acatgcttct
    17281 aagtctttct attacttttg gtaagttctc tagtatataa tctatatcat catctgttgt
    17341 aaaatctcca actgtaagtc ttaatgaacc atgtgctatt tcatgtggta aacctattgc
    17401 aagaagtaca tgtgaagggt ctaatgaacc agaagtacat gcagaaccac ttgaaccagc
    17461 aattcctaac atatctaata ataaaagtat tccttcacct tctataaatt ggaatgcaaa
    17521 gtttgcattt cctggcaatc tattttctaa gctaccattt actcttgtat aaggtattct
    17581 ttctaacaca ccatctatta acttatttct aagacgagtt aaagtttcta catgattttg
    17641 catattttcc ttagctaatt ctgctgcttt tccatatcca actattgcag gtatattttc
    17701 tgtaccagct cttttgcttt tttcttgtgc tccaccatgg acaaagttat gaagtcttac
    17761 accttttctt atgtataaag ctcctacacc ttttggtcca tatattttat gtgaagacat
    17821 actcattaaa tctattccta attctttaac atctactgga atattacctg tagcttgtac
    17881 tgcatcagtg tggaataata tcttatgttt tttagctatt tcggatattt ctttaatagg
    17941 ctgtatagtt cctatttcat tatttgcaaa cattatgctt ataagtatcg tagtatcttt
    18001 tattgaatct tctagttgtt tcaagtctac ttttccttcg ctatcaacat ctaaataagt
    18061 tatttcaaat ccatgatgtt ttgctaagta ttcacatgta tgaagtattg catggtgctc
    18121 tatttttgaa gttattatat gattaccttt gtttttatta gcataagcta caccctctag
    18181 tgtccagtta tcactttctg aaccacctgc tgtaaaatat atttcactag gttcagcatt
    18241 tataagggct gctacttgtt ctcttgcttt atccaaagca tcttttgctt ctcttccgaa
    18301 tgtgtgaaaa ctagatgcat taccaaaata atctgttagg tatggcatca ttgcatccaa
    18361 tacttctttt ttaataggtg ttgttgcaga ataatccata tacaatcttc ttttttccat
    18421 tacattcatc tccaaatcta ttttttaata tttgttatat cattttctaa tttatttttg
    18481 ttatagtcat ttatcatatc tttaagagta attgaatcta taacatcttc tatacccttt
    18541 ttcatctttt cccaaactat ttttgttaca cacatgtttg agttttcaca tacatcttca
    18601 tctagtacac agtcagataa tgcaactggc ccttctaata ctacaagtat atctcctact
    18661 gttatgtctt cagcattctt tgaaagcaaa taacctcctt gagcgcctct tacacttttt
    18721 actagacccg atttttttaa agatgaaaat atctgttcta agtactgatc tgatattttt
    18781 tgcttcttag caataaattt taatgacact ggtccattgt cttgatttaa tgctaattca
    18841 aacattgctt ttagtccata cctaccttta gtagacaact tcatatatta tcacctctat
    18901 atagaaatct aattcatact gttttagtag gaattacttt caagataatt ttattatacc
    18961 ctactaattt tgtcaagatt aattttaatt ttttattttg aataaattcc ccattttcca
    19021 atgttttcaa atcctagttt tttatatatc tttccagcct cttcattatc ataaaacagg
    19081 caaggaattt tgttttctct caataactct gaacatagtt taattaggca ctttgtggca
    19141 agccctctag ctctatattt aggatgtgtc cctactccaa ttatcattgc atgagtccta
    19201 ttttctgaag tactctttgc cattgacact acttgtttgt taatctctat acaatatcct
    19261 ctacctgtcc ttagtccatt ttttaaattc tctaaagttg tactgtgaaa ctcatctatt
    19321 aaatcataaa gtttcactgt cttttttaga tttccaagcc taattctctt aacctttgca
    19381 ttacaattat tatctatcaa aaacttttta gtttgaagtt tgcaaaaatc aactaccttt
    19441 aaattattaa gtcctaatct ctttgctaaa gcatctacag cacatatttt accagatatt
    19501 tcttcataat tagtattacg aagtagattg taaaaacctt caacatcata ctctccatca
    19561 gaataaaaca tcataaattc aaaatattta agtaatattg cctttatttc accaagttca
    19621 tctatatctg cccatatatt caaaaaatta tttccatatc catatctttc aatatcccct
    19681 attataaaca aattgtactc aggttctttt tttaagtatt tcataactct actgtgataa
    19741 gttgagttaa gttttataat cataatatct acccccgata cataaagttc agtctaaaaa
    19801 tctccatcta gatactctaa atattttttt atatttttct cagttttatt ctcttgtggt
    19861 atgtaataga cactatcttt tatgctatct ggtaaatatt gttgttttac ataatgattt
    19921 ttaaaattat gaggatattt gtatccacaa cccctaccta tttctttaga accagaataa
    19981 tgagcatctt ttatactatt tggtatatcc ttaacaaact ctttatccag ttcatccata
    20041 gctttcatta ttgctataca tgcagaatta gattttggag atgtagccaa aagaatagta
    20101 gcttctgcta atggtatttt cgcttctgga aatccaagtt gcatagctga gtctacacat
    20161 gcctttacaa ttacaatagc gtttggataa gctaaaccta tatcttcact agctattact
    20221 aaaagccttc tacaaatact tatcaaatct ccacctttta taagtcttgc tagatagtgt
    20281 atagatgcct gtgggtcact tcctcttatg gatttttgaa atgcacttaa aatgtcataa
    20341 tgactatcac catttttgtc atagtttata atcttattaa atgtagatgc ctttattata
    20401 tctgaatcta tataagttat attatcttta tttggtttag ttgaataaac caccacttct
    20461 aaaatattta aagctcttct catatctcca tcagaaagaa tggctatata ctcaatagca
    20521 tccttattac actcaatatc catataacta tctttattta atacttcaac agctcttttc
    20581 agcccttttt ctatatcaat tttttctaaa ggtttaaatt caaatacagt agacctgctt
    20641 aaaagagctt tgtatacata atgatatgga ttttctgttg tgctagcaat caaggttata
    20701 ctcccatttt ccataaattc taatattgat tgttgctgtt ttttattaaa actttgtatt
    20761 tcatctatat ataacaagac tccacttata ttatttatac ttccaagttc tgatatcaca
    20821 ttttttatat cttctaaaga tgaattggta gcatttattt tatagaaatt tttattactt
    20881 ctttctgcta ttatttccgc tactgtagtc ttcccaacgc caggaggacc aaaaaatatc
    20941 atatttggta aaaaattaga ttgtaatatc ttagacaata tttttccatt acctattata
    21001 tgactttgac ctattacatc attcattgtt tttggtctta ttttatctgc taaaggcatt
    21061 ttctcactcc tttttaataa aaatacttac tttgtcctta cctcaataat tcgtctaaaa
    21121 aaggtatcta tttgaaatag ataccttttc taatctaaac ctataaaata tagattattg
    21181 attgtttttt aattttttta attcatctgt gagtttgtca ttaagtattc ttatatgagt
    21241 acctttcata cctaatgatc ttgattctat cacacctgca ctctcaaatt ttctaagtgc
    21301 atttactatt acagacctag ttataccaac tctatcagct atcttacttg ctacaagtaa
    21361 accttctttg ccatccaatt cagcaaaaat atgttcgact gcttcaagct cggagtagga
    21421 cagagtgcct attgccattt gaactacagc tttctttctc atttcttctt ctaattcttc
    21481 acctattgct cttaatattt caagaccaac aacagttgca ctgtattcag ctattactaa
    21541 atcatcatca ttaaatgaat ttgaatatct tgaaagtact aatgttccta gtctttgacc
    21601 gcttcctaat attggaacta ctgtagtata tttttgtaat cttccatgtt cttcaggaaa
    21661 gatttctaat atcttctcac catttaaatt ttctaatgtt tcatcgattt ttaacacatt
    21721 ttgagtatat tcatctgaaa atttcttttg cttagtatac tcatcttcta taactgaact
    21781 gtcttgagca tcatttaaat gaagacctag tactttacct tttgcactta ctacataaac
    21841 attagaactt aaaacgtcgc ccaatgctcc ggccagtaaa tcaaaagaga cactgcttcc
    21901 accacttgtt tgtaatgttt tatttatttt ccttgttttt tgtaacactt cacttgccat
    21961 ttaatcatct cctcatgaaa tttaggcctt ttaataacct tttatatttt ctaatattat
    22021 tttcacattt aatgaaaatt tatttttcta ttagttgttt acacttgaaa gattatcata
    22081 gattggaacc tttttcaaga gaattatgtg atttttttca attttcactc ttttccgtca
    22141 ttatgctctt ttgtttcttt attacattct ttattagaac acactgtttt tgtttcagat
    22201 ttagtcactt tttcaaccat atatgaacca cacttctcac aaaactctcc tgttggcttg
    22261 ttccaagcta caaagtcaca gtttggataa ttagaacacc cataaaaagc tttacctctt
    22321 ttagattttc taagtataat atctccttct tcacattttg gacatttaac accaatttta
    22381 ttcataattg gtctagttgt tttacagtca ggatagtttt tacaagccat aaactttcca
    22441 aatctaccat atttgataac catattagaa ccacagttct cacaaatctc gtcagtttct
    22501 tcatccatat ttaccttttc tatattttca agagcttctt caatagcctc tttaagaggt
    22561 gcatatgatt cagatacaat ctttttccaa tctgtattac cttcttctat aacatcaagt
    22621 ttactttcca tatcagcagt aaaatctaca tctataaatt tatggaaatt attttccaaa
    22681 atatcagtaa ctattattcc aagttccgtt ggacatagac ttgttccttg cttttcaaca
    22741 tattctctat tcagtatagt agctattgtt ggagcatacg tacttggtct acctatacct
    22801 agttcttcta gcgtttttac taaactagct tctgtatatc ttgctggtgg ttgtgtaaaa
    22861 tgttggtctg gcaatatatt ttctatattt aatatatctc ctttttctat acttggtaaa
    22921 attttgtctt ctctttctga aaaattatat accttagtat aaccatcaaa tttcattttt
    22981 gaaccagttg ctttaaatac aatatctcct attttacatt ctacatttag tatatcaaat
    23041 attgaatctt ccatttgact agctacaaat cttctccaaa ttaaattgta taacttgtac
    23101 tggtctttgc tcaatgaggc ctttatacta tcgggagttc tatcaactga agttggtctt
    23161 attgcctcat gggcatcttg aaccttttta gcatcctttt tttctttttt atcaccagtg
    23221 tttttatagt agttttctcc tagattgttt actatataac ccttagcttt ttcttttgct
    23281 tcatcagata ttcttttaga gtctgttctt atatatgata taagaccaac agtaccttct
    23341 ccttctatgt cgataccttc atataattct tgtgctatca tcattgtttt tttagtagta
    23401 aaaaataact tattcgctcc ttcttgttga agcatacttg ttgtaaatgg ttttggagct
    23461 gatttacgtc ttgatttaga ttctatcttc tcaaccttta aatctttacc ttccatttct
    23521 tttagtattt cattcacagc ttcttcattt gctaattcaa ttttttcatc atttttacca
    23581 taaaatttta aagttatatc tttgtctttt actgtcttag cctcaacatc tattgtccaa
    23641 tattcttttg gaataaatgc ttttatttct ttttctctgt cacaaattaa ctttgttgta
    23701 acagattgca ctctacctgc actaagacct tttctaacct tttgccataa tataggactt
    23761 atttgataac ctaaaagtct gtctagtact cttcttgcct gttgtgcatc aaccaaattt
    23821 atatcaatat ttctaggatg tttaattgct tttttaattg catccttagt tatttcatta
    23881 aactctattc tacaatcctc agattcatct aaatttaata tatgagctaa atgccatgat
    23941 atagcttcac cctctctatc agggtcagtt gctagataca cttgtttaga ttttttagct
    24001 tcttttttta attcctttat tacatcgcct tttcctctta tatttatata ttgaggttca
    24061 aaattatttt ctatatctac acctaattta cttttaggta aatctctgac atgaccaact
    24121 gatgctttaa ctgtgtaatg acttttccct aaaaattttt ctatagtttt agcttttgca
    24181 ggcgattcta cgatgactaa tgttttggcc ataaaaccat acacccccac tacctaatct
    24241 taatttatac atttaaactg tacgttttat tattcatttc tactactaac tcatttagta
    24301 ctaattcatt gattattgaa tttacatact ttatttctat accagtataa tcacaaatac
    24361 tatctatatg caagtttcct tctcttttta tagcttcaat tatttgctta gacttggcat
    24421 ttagacttat attatcatat ttttgattta tttttttatc aataatatta aatatgttat
    24481 attcatttaa aatatcatct atattttcaa ttaattttgc cccttctttt ataattttat
    24541 gacaacctct actcatttga gaatttatat tgccaggtat tgcaaaaaca ttttttccat
    24601 gttcaagagc aaaatctact gtgattaatg caccactttt tattgctgct tctacaacaa
    24661 ctactccatc acttaagcca ctgataatcc tatttcgatt agaaaaatga tatggggcta
    24721 ctgtcgagtt aatattatac tctgataata ataatccccc attttctaat attttatttg
    24781 ataaatgtaa attttgtttt ggaagaggat tgtctactcc tgaaccaagt actgctatag
    24841 ttttagattt tccactcata caacctatgt gcgagtatgc atctattcct atagctaatc
    24901 cacttactat attaactcca tattgggata gttgacaact tattgttttt gcacaattaa
    24961 ttccataggc tgtaggtttt cttgaaccta ctatagctat attaaattta ttattaacta
    25021 aatctatgtc acctttatag aataataact ttggagcgtt gtatatattc tttaaatttt
    25081 caggatattc tttatcatat tgacaaatat atttaatcgc ttttttgtaa agtagttctt
    25141 ttatattttc taaataagcg tgacctctat attttactat attttttcta atattcaagc
    25201 ttatattttt taaattatat atttcttttt cagaaaaatc aaatatatct tcaatattta
    25261 ctatttcatt ttctattatt tcaattgttt ttgttgttat tccaccaatt gattttaacc
    25321 ataaataagt atctcttttt tccataaaat ccacctcaaa acactatcta aaatagctat
    25381 aataagcctt tctaaatgaa aaagcttcta taatacactg tgaatttatc aagtctcttt
    25441 cttctaaatc tgctattgtc cttgccattt ttaaaagttt tgtataactt ctactgctta
    25501 ggttgtattt attaaatatc atctcagccg ttttcgaagc ttctttttcc agtttacaat
    25561 atttgaataa attgtaagct ttaatttcac tattattttt tatattatct ttcttaaatc
    25621 tgtttatctg tatttttcta gcattttcta ctcttctctt tattttttga gaactttctt
    25681 cttgttttaa agaattaaaa tctgaatatt ttatagaatt aacttctaca aaaatatcaa
    25741 acctatctaa caatggacct gatattttat ttatgtatct atcaatttcg ttacttctac
    25801 atctgcactc tgtttctgac atataataac cacatggaca tggattcata gctgcaatta
    25861 ataatactct acaaggatat tttgcactgt actttactct agatatattt acatatccat
    25921 cttctatagg ttgtctcaaa gtctcaagta tttttctatc aaattcagct atctcatcta
    25981 aaaacaagat acctctatgt gctaaagcaa tttccccagg tcttgcatcc attcctcctc
    26041 caattaaaga ttgctttgta gttgtatgat gaggtgctct aaaaggtctt ttatcaatta
    26101 ttcctttagt ttcatttatc atacccaata tactataaac cttacttact tctatcattt
    26161 cttctatact tatatctggt aaaattgtcc ttactctttt tgcaatcata gtttttcctg
    26221 aacctggagg tccaatcata aacatattat gatttccagc tgctgcaatt tcagcactcc
    26281 tctttacaaa gtaatttcct tttatatctt caaagtcttc attatattta ctttcttcat
    26341 cattaatctt agtatatttg aagtctatat aactattatc tttttctaat tcttcattct
    26401 ctttttttga cttatcattc aaaaaagaca ttatccttac tttatcgact tttatttctt
    26461 cgtttaaaaa atcgacacat tcttttaaac tcttaatagg aattatgtca atttcatcta
    26521 caaatagact ttcttttatg ttctcaattg gtataaaaat acgctttatt ttctgagttt
    26581 ttgcacccat aattataggc aatatacctc ttacttttct tatttttcca tctaatgata
    26641 attcgcctat aaacatactt tctcttatgt aattttcatc tttttttatt aactctctta
    26701 aaagacctat acttatagat aaatctaaaa aagctccttc tttttttata tctgctggtg
    26761 ataaatttac aactattctc gtgcttggaa atttatatcc actattaaga attgctgact
    26821 ttactctttc tcttgcttct ttaatttctt tacctggaag accaacaata ttaaaactag
    26881 ggattccatt gcttatatct acttcaacct taactaaaaa gctgtcgatt cctactaagt
    26941 tacttgaatt aataatactt aacatattta atcctttcca aaaacattat cttttttaaa
    27001 aagcattcac tatgtgattt attctttttt ctaataagta tacctctata acatcaaatc
    27061 ttacgggagt attatttaag ttatttttta taatataata atttgcaatt ccaattattt
    27121 tttttctttt tttgtaactt acagattcac aagggtagcc aaactttatg ttagaccttg
    27181 atttcacctc cacaaatata acttctcttc ccatttttgc tattatatct atctccccta
    27241 tctttaatct gtaatttttt tctagtatcc ttgctccttt tgatagcaaa taattaactg
    27301 ctatttcttc tccaaaatca cctttttctt tattattcat gattcactcc aatacattaa
    27361 attttcactt ttaaattatg gtcagtatac aattttacta ttcaattttt gggattgatt
    27421 ttatttctat caatattatt aaatacttct attgacttta ttcttttatg atattattct
    27481 agttttcata ggaaaagttt atttttttgg aggagtgcct ttaatgaaca gaaaaaaatt
    27541 agttgctttt atacgatatt tatctttatt tatattagtt gtaagctatt tgaaaaatgt
    27601 tgaaaacaca ttgtatccag tcataatcat gatgatatta tttataatta taataaataa
    27661 tcaagttaga tttttttctt tatcaaatga gaataaattt atacttattt cttacttttt
    27721 agaacttata ttaattttta tactgtgtaa ttatacaaaa acttttaatt cgatttactt
    27781 tgttccttta atattagata tatcattttt aataaaggaa aaatataagt acatattgtt
    27841 cactttagtt ataatatctt ctcttattat tagtttagat aaaaatatat atttggcact
    27901 ggaatcaagt tctgtattac taattataac tatactttct atatatatag aaaatgagaa
    27961 tttatcaaaa ttatacagtc aaaatcttta tgataagctt agaatttcca aggatgaatt
    28021 aaaaaaggtt aacgctgatt tggaaactta tgcaagttct atagaagaat tagcaatttt
    28081 aaaagagaga aatagaatat caagagaaat acatgatagt gtaggacatt ctctttctac
    28141 aacaataata caattaaatg ctatagaaaa gctcctaaaa gacaagcctc taatttctga
    28201 attagtacat gaacttagag aatttgtaac tgaaagtttt caagatgtaa gaagggctat
    28261 atctgagtta aagcctgttg aatatgaaaa ttaccaaagt ttatttaaaa tcaaagaact
    28321 agttaaaagc tttattaagc ttacaaatat caatgttaaa ttaactattt caaaaaatac
    28381 ttggaactta tctagaaatc aatctatagg tttatataga cttatacagg aatctctttc
    28441 caactcatca aggcatggaa aggcaactga aatcaaaatt tttatgacat tcaatccttc
    28501 aaattttata attactatta gtgataatgg tataggatgt ggcaatataa aaaaaggaaa
    28561 tggacttaat agtatcagtg aaagaatata tgaattaaat ggtaaagttg aatttgataa
    28621 ttctaataat ggtttcacaa ttagagcttc ttttcctaaa ttttcaggag gtgatttttt
    28681 tgaataaaat caaagttctt atagtagatg atgaaaaatt aattaggaaa ggtcttaaaa
    28741 ttatattatc atcttataat gatttagaaa ttgtaggaga tgcttctaat ggatatgaag
    28801 ctttagaatt ttgtaaaacg aatgatgtag acatagttct tatggatata cgtatgaaag
    28861 tatgtgatgg agttctaggt acaaggctta taaaagaata taataattct ataacattat
    28921 taattttaac aacttttaat gatgatgaat atataaaaga tgctatgaag tttggagctt
    28981 caggatacct attaaaagat agctctgatg aagtattgca tgaagggatt cgttcttcat
    29041 tttttggcaa tattgtcctt gataaaagtg tagctgaaaa aatcatgacc cctgaaaaaa
    29101 tgattgaaca agagcatctt tatgacatgt acaatttaac agaaaaagaa atatccataa
    29161 ttagactaat tgcaaatgga cttaataata aagaaataag taaagaacta ttcttgtctg
    29221 aaggtacaat aaagaataac ataactaata ttttaggtaa actagaactt cgtgacagaa
    29281 ctcaactagc aatctttgct ttcaaaaata aaattgtaat agaataaatt taaaaatata
    29341 aagtgactta agtcactttt tttagtgact tatagtaata gaattataat tcataattct
    29401 ttattatagt attatagatt tttaacttat acaaaattat tttggaggaa ttattatgga
    29461 tatagtcaga gtaaataata taacaaaaag atttaatgat aaattagcac tcgataacat
    29521 cagcttttct gttaaaaaag gtgagatatt tggacttatt ggtccaaatg gtgcaggtaa
    29581 atctacactt ataaatataa ttacaaatct tatgttacca aatagtggta gtattcaaat
    29641 aaatggttta gatttatcaa aggattatat aaaagcaaag tctataatag gattagttcc
    29701 acaggaatta gcaattatag aaactctcac tccttttgac aacttagagt actttggagc
    29761 tttctatggt ttaaaaggaa atttacttaa agaaagaatc aatgaagcat tagaagtcac
    29821 aggtcttact gaagtcaaaa agaaaaaagt caaaaaatta tctgatggta tgcaaaggag
    29881 attaaatatt ggaatagcac tcttaaatca tcctaaaata ttaatattgg atgaaccaac
    29941 tgtaggtgtt gacccccaat caagaaatca tattttcaat tttataaaag atatgtctaa
    30001 aaaatatgaa actactgtaa tatatacttc ccattatatg gaggaagttg aacatctttg
    30061 ttctaaaatt tttattatgg atgaaggtaa agagattgct tttggagata atgcttattt
    30121 aaaatcgcta gtatctacaa atacaaaatt aattatggaa ataaaaaata taaatgctca
    30181 acttatcttt gatttaaaaa atactaaagg tgttatatct attttagaaa ataattcatt
    30241 attagaattg gatatagata aaaaattaca acttacagag atactctctg tcattgacaa
    30301 aaacaactca aaaataataa gaatttctta tgaagaacct aatctagaag acgtattttt
    30361 aaatctaaca ggaaaaaatt taagggacta atggaggtaa ttttatgaaa ttatacatga
    30421 gtgtaaaaac tatgctaaag ggtttaaaaa gctcctttat acttaattta atatattttt
    30481 tagctttacc tctgatttta tcttggtttc ttgggatggt tacagaaagc atgtttcaaa
    30541 atcctataaa aacagaatct acacccattg ttatatatga caaagacaac acaagattat
    30601 caaatcattt gacaaagtat ttgaaaaatg atttatctta tatattaaca gttaaaaaag
    30661 atgattctaa ggcagaatta aagcttacta tacctaaagg ctatgagagt agtttattaa
    30721 atgaaaaatc aaatactcta aatatagaaa aacttggaac tcgtgatgat attgcaatat
    30781 tgcttcaaga catattagat acttaccatg aaaaatttta tttaaataat tcccagaaaa
    30841 taagttcaga agatttctct aaattattta ataaaaattc tattgatact tctataatag
    30901 gtaacaatgt taaacagagc tcttatgaat actttgcttt agtttcgcta gggtttcttg
    30961 taataatctt tataatgaac aatattttat caaattatat aagcgaatca aaaggactct
    31021 caaaaagact ttattctatg cctataacta gagtacaatt cttaatttat gactttgtag
    31081 gtttatggat ttattcattt atattcttat tattatatgt tttattcttt agaataattg
    31141 gaataacatt taaaggtaat tttgccatat tgctcctact ttgtgcttta tcatcttatt
    31201 tcatgacttc aatatctact tttgtaaaca gcttttttag taaaaaatat ggtacaataa
    31261 tagtttatgc attactcttt ttacagacaa tttttggagg tatctttagt atgataagtg
    31321 atgcatttac taagcttaca agtttatctc ctacctattt aataggagaa ttgtttagta
    31381 attatgaaac atttaaaact atagattcaa taggaaattt aattttcaca tgtttaatta
    31441 cttctacaat attgattgca cttgctattg taaaggaaaa atataaatgg agggaggttc
    31501 aataatgatt agattaactg ttgcaaatat aaaaagatat ctaaaaaatc atactctttt
    31561 aattaatatg gttatgcttc ctattatact aattttttca cttaattttt ttatcaacaa
    31621 ctcgggaaat caatcaatgt acttttctcc agtagcaatt gtatctgact cttcaggaaa
    31681 gtatgaacat aagttaataa atagttctaa gttaaaagaa aattcattta gattaaatga
    31741 acaagataag gctatgaatc tattaaaaaa taataaggtt tctgctgtat tcgtcttaga
    31801 taaaaatttt tcaaatagta tagataactt acaacgacct gtagttaaat gctttaaaat
    31861 tgaaaatggt ggtggtagtc tttgggcaga atcacaaata gaatctttta ttactaaatc
    31921 gttaaaattg aaaatggata aaaatataga tgataaattg acaaagacaa atataattga
    31981 taacaaacta gataagaata aaggttcatt tttagtagtc ttcttgatat gctattttat
    32041 gtatattaat gcggcacatc ttgcttctga tttatttaca ttaaaaaaat caaatgtgct
    32101 aaaaagactt atctctacag ataataaaga tataaaaata attttcagta tatttttagg
    32161 gttatttttt attcaatcaa ttgtatacac ttttactcta ttatgttttt caattataga
    32221 agactttaat ttaagtctga atgtattgat gattgtacta gcaaatagtt ttgtttccac
    32281 tggatttgta ttttggatag ctagagtatt taaaaatgaa agttctataa gtcttatatc
    32341 tacattttat tctttaatag gtttggctat atctctatct agtctaatac catcaatgga
    32401 taaattatca tttatggcta atctttcaaa acttacacca ttttattgga ctatagatgc
    32461 tattaaaaac aatggtagta tcttaataaa tataataatt ctgatattga ttggaactat
    32521 atttgtaact gcaggaagtc ttaagttacg agattttgct aaaaactagt agttattaaa
    32581 tataattagt atttattttt atataatgat aaaagaatat tccaacatgt taaatcattt
    32641 taccttatat ttttctattt agttttatat accatataac ctaaacttaa tattatatat
    32701 taacattttt gacttgtcaa gttttttatg gtataatatt aattattaga taattaatat
    32761 actgtaccta agttgcaata tctaaaattc agtttggagg tataactaat atatgtataa
    32821 aattggtgaa agtgtgatgt atccaaaaga gggagcctgc tgtgtaaatg atattgtaac
    32881 aaagaaaata aatcatcaga tgcaaaaata ctatgaatta agtgttattt tcaatagtaa
    32941 ccttaaaatt tcgattccag ttctaaatgc agacaggatt ggaatacgtc cagttatgga
    33001 tggaaatgac gttgataaat ttattcaatc tattgataaa acagatggtg tatgggtttt
    33061 tgatagaaaa caaaggctta aactgtacca tgataaattt cattcaggag atgtttttga
    33121 gattgtaaaa ttaattaaaa tgcttatgat acaagactgt tcaaaacaat tgtgtagtac
    33181 agataaggag tttttaaata aagctcaaag atttgcttta tcagaattgg ctgctgctca
    33241 atgtaaatct tatacagtag tattagaaga aatgaaaaaa catatactaa attctaaaag
    33301 taaaaattaa accagtctta aagactggtt ttatttttat actaatcatt tatgctactc
    33361 tatcttactt taaactgcct aatctaatat taataaggtt accctaagta gatagatgtc
    33421 tttattcaaa taaaaaaact aagagtaaat catattactg gtttactctt agataataaa
    33481 acttaatctt cttcaagttc ttttttctct tgtaataata cacttaattc atttgcttct
    33541 aaaacttgtg cctctgcaat atcaagtaac tcttgttgcc attccacaca tttatctttg
    33601 taatcaaaag gtagcgttct gtcaactatg tacttatcaa tacagtcctc cgcactttta
    33661 attatttctt ttctttcatc aataaatttt tctaattcta ctaaggcttc ttcataagct
    33721 tttctactca tataaattcc ccctttgtac aatttattct acattaaaac gatttttcct
    33781 cttttttgta taaaatttta tttgtatata acttttccat caatatcttt tatagtttga
    33841 catccagtta atatcataga gctacttagc tctgattgaa gtttatttac ataagtttct
    33901 acaccatcag ttgcaccacc aaatgaagca gtcacaaaag gtcttcctat taatactgca
    33961 tctgcaccta aacctatcat ttttagtacg tctaaaccag ttctcactcc accatctgcg
    34021 agtattgtaa ctttaccttt aacagcatcc gcaatttttg gtaaaacttc acatgctcct
    34081 ggtgtacaat caagtactct tcctccatgg tttgatacaa ctattgcatc cacacctgct
    34141 tctactgcca ttaaagcatc ctcaacagtc attatacctt tcaatataaa tggtagttta
    34201 gtagacttta ctaactcttt tatttgttca acatttttag gaagtactgg ttttccatgt
    34261 agtgataaag ttactagacc gcaagcatct atatcaactc caactgcaaa tgcacccgct
    34321 tcttcagcta atcttatctt ttttataata ttttcattat cccaaggttt aataaataca
    34381 attccagctc cattatactt tttaactaca tctaaatttt ccattaaaaa agcatctact
    34441 gctgtatcac caaccattgc ataaatacca ctattagcac atcctgcaac tactggttct
    34501 atgtaatctc tttcattaat ttttccaccc atatttaaag tagttcctgt tactggagct
    34561 gcaaatatag gtgcactcat tttctttcca aacatctcta tagatgtatc aggatttgat
    34621 acatcatgta ttactctcat atttactttt actttctcta aactcttgct attttctata
    34681 aaagaagaac cacttccttt acctcccatg ccaggtactt cgcctgcaca agcaacccca
    34741 ttgcaaacct tacaaacttt acaacttcca tttaaatttt ctctagcatt ttttaataat
    34801 tcattatatt ccatctttaa tcccccatta attatagtaa tttatattat atacattata
    34861 tcatattata tttacagtat atttttcaaa aaactttttc tgtggatagg agttatgcca
    34921 tatttttcaa tagcttcata gtgttcttta gttccgtatc ctttgtgaga tttaaatcca
    34981 tattctgggt atactctatc atattggtac attatactat ccctagtaac tttagcaagt
    35041 atacttgcgg ctgctattga tatagatttt gaatctcctt ttacaattgg attttgagct
    35101 atatcaatac ctggtattgt tgctgcatca actaataaat aatctggtgc tttttttaaa
    35161 caatttatag cctttttcat tgccatatat gtagcattta atatattaaa ttcatctatt
    35221 tcttcattgt ttactatacc tattccataa tctagtgctt cctcttttat tacttcaaaa
    35281 agttcatctc tttttgcctc actcaatttc ttagagtcat ttacaccttc tatttttgta
    35341 tcttttttaa atactacaac agaagctaca acaggtccag caagtggccc ccttcccgcc
    35401 tcatcaattc ctccaatata taaatagcct ttattatagc cttcgttctc aaatatattt
    35461 atagtttcta atctctcttc ttcttttcta atattatcta gtttttttgc tagttttata
    35521 gctaaacctt gtacagattt tctttcatct acccttaata gttctatata ttccatatat
    35581 ttttctactt ctaaagtctc tattatttct ttaatctccc tcacactttt atcctgcata
    35641 ccattccccc atctttcgtt tttaaaaatt taagttttaa acatattaaa atttcttaat
    35701 tagagtttaa ttgcatccac tataaccaca ttttaaacat atactacatc cacctgtatg
    35761 tgcaattcct tcacttccac actcagggca cacaaatttt tcatcttctt caaaattttt
    35821 atttattatt tctttggctt tttcatcttt attatctttt tcttttttat tatttttata
    35881 atattctttt ttactatttt cttttttatt ttctttatta ttttcttcct ttttttcatt
    35941 tattaaaatt ccactccttt tacatccatc tctaaatatt gttactcctt taagaccagc
    36001 ctcccatgcc ttaatatata tattgaaaac atcttcaact gtagtttcaa aaggtatatt
    36061 tattgtagat gatatagaag catcaatata cttttgccat actttttgca tgtttattct
    36121 ttcttcatat cctatattca tagcagtaac aaaaaaatct ggtaaacatt cttcatctgt
    36181 aatacctttc ttatccatat actctttaac aattggagta tatactttat agtacacatc
    36241 ttcatcatgt aagctttcag tttttcttat atatgaaaga ttaaatatag gttctattcc
    36301 accactaata cctatcattg tagatataga acctgtagga ggtattgtta aaagttgtga
    36361 gtttctaagc ccatactttt taaccatttc cttagtgtct tcatttaaat tttctataaa
    36421 aaacttagat ttagaaatat tttcatattt atactcacta aaagtgccat attctttagc
    36481 aattaatgct gattgcttta cagcttcatt taacatagtc ttaccaatta aatcacataa
    36541 ttctaaagat tcatcagagc catatcttat tcccattttt ataagcatat cagctatacc
    36601 cattacacct aaacctatct gtctatattt atgtacacta tctctttgtt cttgtaatgg
    36661 gtgcaagttt aatccctcat ctaagacatc atttaaagct actacagctt ctctaacgca
    36721 agattttagt ttattaatat caaatgtagc atgttcacca aatggttgta ttataaactc
    36781 tgataagttt attgagccaa gtagacaact acctccactt ggaagtggct cttctgcaca
    36841 aggatttact cctgaaaagc aaaagttctt atcttcactc ataagattat atgtttttat
    36901 tttatcccag aataatatac ctggttctgc ataatcccag ttatttttca caaatttatt
    36961 aaataaatca tacgcattta ctatcttaac tattttttct ccagtagttt caacttcaaa
    37021 agtacaatta tataactttt tttcccttac agcttgcata aattcatctg ttactcttac
    37081 agaaatattt gctttagtaa ccttctccaa atctgttttt atatctataa actcttctat
    37141 ctctggatga tttacatcca tagaaatcat aagagctcct cttctccctc tttgaccaat
    37201 taagccagtt gtcatactat aaagttccat aaaagagact gctccagtcg tatgttttgc
    37261 agcattgtta actttcgctc cttttggtct tagctttgaa atgtcaattc caactcctcc
    37321 accataacta aaagttctag caagtttttt tgcagtatcg aaaatatctt ctatgctatc
    37381 ttttggtggt tctattacat aacaattgga atatgttact ttttttcctt ctttctgtaa
    37441 tcctctattt gctaaaattc ttccagcaaa tagaaatctt ttttgtctta ttagttttga
    37501 tattcttgaa ctattatttg aaattctctt tatccattca tcaaaactct caccattatg
    37561 tctatacttg ttcttccata ttgatttttg taattcatca aactcccaag ctttagttct
    37621 tatttctgag cgtctttctc tatataatat gtagtgtttt gctatatctt ttctatttga
    37681 ttccataagt agtacttcaa ctatatcttg tatttcttct acggataagc atttttccat
    37741 agaattatag tcattggcta tttttcttga tatatcatat gccaaatctc tatctactcc
    37801 accttcacta tttatactag ctttatagac agcatcttca atcttacttt tgctaaaaag
    37861 ttcttttgta ccatctcttt ttattatgta cttcattttt agacctccaa taaattagta
    37921 ttaaattaat acacacatta actataactc taaacaaact ttttatatat ttgtatattt
    37981 tcatattaat tat
//
LOCUS       DAMJVM010000028        37468 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.028, whole genome shotgun sequence.
ACCESSION   DAMJVM010000028 DAMJVM010000000
VERSION     DAMJVM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37468
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.028"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            <1..99
                     /locus_tag="PC523_001542"
     CDS             <1..99
                     /locus_tag="PC523_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2475077.1"
                     /translation="ICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            217..993
                     /locus_tag="PC523_001543"
                     /pseudo
     CDS             217..993
                     /locus_tag="PC523_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
     gene            1148..1909
                     /locus_tag="PC523_001544"
     CDS             1148..1909
                     /locus_tag="PC523_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475078.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTVEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            1912..2730
                     /locus_tag="PC523_001545"
     CDS             1912..2730
                     /locus_tag="PC523_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HDF2475079.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            2909..3511
                     /locus_tag="PC523_001546"
     CDS             2909..3511
                     /locus_tag="PC523_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HDF2475080.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRVEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPGGIKNMIQDYLGEIIESEVKKSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIVQGLVTMFI"
     misc_binding    4001..4240
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4304..6223
                     /gene="thrS"
                     /locus_tag="PC523_001547"
     CDS             4304..6223
                     /gene="thrS"
                     /locus_tag="PC523_001547"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDF2475081.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSNEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            6948..8039
                     /locus_tag="PC523_001548"
     CDS             6948..8039
                     /locus_tag="PC523_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2475082.1"
                     /translation="MIEKVGEKSKIIKTSKEYKRTIIEEKDREWTQKLNDILSRNNFS
                     NDELDKIKELIPKEILTSENVGEVVTEDGYARPEYDEVFKSLFTLNNEYGLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRLETDPTINYIGEKRPRLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPEIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLIINPSSLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNAFDLSEDEIKEVYENN"
     gene            complement(8387..8584)
                     /locus_tag="PC523_001549"
     CDS             complement(8387..8584)
                     /locus_tag="PC523_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2475083.1"
                     /translation="MTIASDVIRCDTKTSILKHLLKKDSYSYEINKSILEKTKGLYEL
                     KEAILYTAFRRLEQSGAITSY"
     gene            8944..10284
                     /locus_tag="PC523_001550"
     CDS             8944..10284
                     /locus_tag="PC523_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2475084.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKKISNKKINFCI
                     VGIIVFANIIYFFDAPLNIRVIISMIVTIAFYKISYDVRFIKSVLISLIYWMLLIAID
                     ALSISLTVWLNSINDMSNMGTGDIYRIESIILGKILLISALFLYRALKIKFNIEINKR
                     TLFYIVIPIVANIASFFVIFKYIFNLKFGNLIHNFQFVVISVVLLVSSITLILIAKKL
                     QQYNTLVVENNMIKNTLKCENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDRNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDIMQNFYLIKVENTKTNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKVVNNII"
     gene            complement(10626..11195)
                     /locus_tag="PC523_001551"
     CDS             complement(10626..11195)
                     /locus_tag="PC523_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2475085.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
     gene            complement(11356..12606)
                     /locus_tag="PC523_001552"
     CDS             complement(11356..12606)
                     /locus_tag="PC523_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2475086.1"
                     /translation="MVKSLNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIIFSPIGGAIADIFNRRNLMVIFDFSSSITVLVLALFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQTSNIIKTIFIDINDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKYLKITNLYISFTICALMFIPMAFSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYAITIIQLKTPNELLGKVMSILFAGTTIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLIIAFISKITLRNEKL"
     gene            complement(12615..12848)
                     /locus_tag="PC523_001553"
     CDS             complement(12615..12848)
                     /locus_tag="PC523_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475087.1"
                     /translation="MIELIQRLNDVIYKINDKLNIHTKLVDPSSELMPLYSMLSIIVS
                     IFLIILGIKVRKKVLIVIAIIAIIVNVLIILDR"
     gene            complement(12899..13477)
                     /locus_tag="PC523_001554"
     CDS             complement(12899..13477)
                     /locus_tag="PC523_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2475088.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGTFYTFFDSKEKLFFEVLNGIQNRLISSVDEILGNMPQKEGFAKALKMLYRLYDKN
                     PFLYSPNNTDYITLLNKIPKEMLSQLEYNNVQSFYSLIERHNLKLKIEKEKAFGVCNA
                     LLSSLLLKEDIGYDYIEVFDFMVDNLVEHILE"
     gene            13778..15238
                     /locus_tag="PC523_001555"
     CDS             13778..15238
                     /locus_tag="PC523_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HDF2475089.1"
                     /translation="MFNELRTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEAIKNTKESIKVWADMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     MKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVEKIKPLVENLTIGNPKDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLIVGNIRKNNLFYPTLLDNVNTDMKIAWEEPFGPVL
                     PIIRVKDISQAIEIANQSEYGLQSSVFTSDIDRAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVRGL"
     gene            15607..16212
                     /locus_tag="PC523_001556"
     CDS             15607..16212
                     /locus_tag="PC523_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2475090.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDDELSPQQCLRKIMLNHLRFP
                     KQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRLSDSSEKMLDETMNIFLHGIMNDGGE"
     gene            16215..18782
                     /locus_tag="PC523_001557"
     CDS             16215..18782
                     /locus_tag="PC523_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDF2475091.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGVLFHNGEMPQEVSDAIKTAY
                     DGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDEVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYTLTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     FLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIALQSEKE
                     LWGLSEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAYSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEALIEQLDKNKSLSDMNGLIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     ALQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(19042..21126)
                     /locus_tag="PC523_001558"
     CDS             complement(19042..21126)
                     /locus_tag="PC523_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2475092.1"
                     /translation="MSKYIDFANIEYSLLMNALDVSVSKHLLDEHFTVVWANDRYYEM
                     FGYTKEEYETLFHNQCDLFYKDNPEDWNELVEYVEKTFSTGAKKYDYVCRMKHRNGKK
                     LWIKLFGFLTGEVVDGYPISYSVMIDITEQMMIKIEQTVTYNNFPGLIAKFKVTDFGF
                     QLLDANKKYFDMFKDNVNFLLDDINLESYLSAVYKYQSDFKAGKYVCFSVSPQNINGD
                     IVHMQVVAECIDWEDGKPIYLLIYSDVTTLVKQNEIIEKNNKELEKLAFIDSVTGGMN
                     HTKFNIIAKEVISQSPSGTYTLIWLNMQKFKLINDLAGNIAGDKLLKYIYNCISKHLH
                     EGELVTRIFADKFILLLKDKPEDITSRLNSMVTHINRFNDNADYKYILYFTAGAYVIY
                     DTSLDITNIQDSANVASKSIKNVENHGLCICNFYSKKDKEKLILEQSIENRMYEALEK
                     REFQVYLQPKLELSTQTICGAEALVRWIHPVEGIIPPNEFIPLFETNGFIIQLDLYVF
                     EEVCKTLKKWIDDGLNPINISVNMSRYHFSSNNFIKTYDKIAKKYDIPSHLIEIEITE
                     TIVFEDVKVFSEIINEIHKNGFKCSIDDFGSGYSSINMLKDIYVDTLKLDRAFFQSEH
                     LDSQRERDIIVSIIELAKKLKMTTVAEGIETSFQKDFLQEINCDMLQGYVFSRPLPIK
                     DFEKLMFQNSSN"
     gene            complement(21174..22055)
                     /locus_tag="PC523_001559"
     CDS             complement(21174..22055)
                     /locus_tag="PC523_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2475093.1"
                     /translation="MPAYLFDSNYITNIYRVYSISSSFIVTGLYFCYKNHKNNQINFD
                     TVLEDKKNLNFLLSLMFVMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     KYSFRITQIKSYDIKNQLEYLESIKTEHEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     MSNHYKFTLCFTDLNRLKLVNDNFGHQFGDKYISTTAKIIKKLINVENSLLFRYGGDE
                     FLFLLQDTSCIEAEKIMIDINYELSKLSNTDEYPYALSISYGLVEWDGISDIETLIAE
                     ADSLMYENKLKNKSTLN"
     gene            complement(22405..23586)
                     /locus_tag="PC523_001560"
     CDS             complement(22405..23586)
                     /locus_tag="PC523_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2475094.1"
                     /translation="MKYHIFLIITIILIVELCNIYIYKIASFGNKSIAIHKNYKYIFL
                     IKPLTFLTIIFTKLPLPLFYILFYIVSHKLLLISIKNSDNKHFIPNIAVINFCILHLI
                     TISIMSIVENTSPDIIFVNMSSRIISILIASILDFILWYSMDKIKEHIYVITQNQIEF
                     SLFLKFIYFTLGYILFDSIQIAFSPPSALTSYFILGSNILLSLQMIIFINHIYSIEKN
                     IYLEKEHNRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNSLLFSL
                     IYIDLDKLKTINDKYGHLAGDNYIINFSSIIISNLKADDIFARIGGDEFIVIMPKSNE
                     NTTKEFIEKIRKSMYNKISFSYGIVEVPSENKMTVEEIINIADIRMYTNKQNNHKRNS
                     N"
     gene            24713..24988
                     /locus_tag="PC523_001561"
     CDS             24713..24988
                     /locus_tag="PC523_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475095.1"
                     /translation="MSLIVGGWSKFRAVEEEDLKVFNEAVGMLKGVDYEPLIVATQIV
                     AGTNFKFICNATSVTNPPHDYLAEIIIFNPLPCDKQKATITSINIVK"
     gene            25008..25295
                     /locus_tag="PC523_001562"
     CDS             25008..25295
                     /locus_tag="PC523_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475096.1"
                     /translation="MRLNITKQNTIEVEGSKKEDVYFIIVDNVFFERKGNVFYSITNN
                     NLNNYDIKILPTDNIFLELKITSTKDLEPVFIVAEDRQYNHCSVMFKPVMV"
     gene            25715..28231
                     /locus_tag="PC523_001563"
     CDS             25715..28231
                     /locus_tag="PC523_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2475097.1"
                     /translation="MPEDNKTFLYELKELLSNGEKQIAKAIGQDLILILGGTGCGKST
                     AINYLAGCEMHKVEDEDTGVSYITCEDPVADIGSGAVSKTLYPEVIDMKKGFEGMTAK
                     FCDTAGFGDNRGAIHDICAAVSLGEVFKDSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKNSITFFISKSFLGRTEKQIYNVIKRKYEQLCEASDSNMSDSNSNIK
                     EKAWLFEEMLKDDGKNIHICNPLASDDREGLLRQVLELEEVKDKISAFQYPISADALL
                     VLDTLIKEIQDNIVSSLEDYVKAYTEDFISAVGSITMIEEIAKYDDVVEVFKNWLEST
                     TSPTIGRFINELARQSSLFLQDSCMKLQKNIKENLDIIDALSEYTEHGTSEILLSSVH
                     MEFIIEKTSKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNRENLMEVANKKDYKYS
                     VNDFNSILTNVTVTQKEELEDSITRLNAYGEEGNEIIREFISKYILNPFIVKTININD
                     KQGIEIRALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKNTIYMDDNLGMDFASEKNI
                     IIACNNLDVGSNVKINVSGKSGELVDNSMYGKNGEDGRDSGNIYLYIKEEIRNYRLTL
                     IANGGNGSKGIDGKNGETGASGSNGADAGYTSSFHGKMFKGYFAQGTSGGKGEKGGAG
                     GNAGRGGNAGKKGNIYFISDSEEQKNRIINRCDITVRDGEVGPNGRPGSGGYGGNGGY
                     HGYNTLSFSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPGREYAE
                     YGDRGADGGFNAPNTKISTAVSKIEDNFIKEKYQCYCSEI"
     gene            28246..33456
                     /locus_tag="PC523_001564"
     CDS             28246..33456
                     /locus_tag="PC523_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDF2475098.1"
                     /translation="MANKLYSGIVNLLEDGRDELRKYNLEKKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDAETGQVYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRYTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKALISNVTEEKIRNILNKTKEDLDGQEIDYDGCKELIYL
                     LDVMLSDSVSIELCNPLDKNPEKLLDKMKNLPETFEKTNLGLMLTASCKIEIQKAVDV
                     TYKELEKLLVPYIESVKKGMEDSISTLSSINSLKDKKIQLDRMYTDLCGRANSMKELM
                     EFVQELSYGDGMQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYKEEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLIK
                     IISGYKLDILSSIYMYGDCELKIDCSMEKDVFSGKNIVLLTEKVFIPEGSYSINVSGK
                     NAEKLAGEDYIYNGMNGRNGNNAGTSGDIILSFKEIEKIGELKLYVDGGRGGNGQDGK
                     IGETGLTGKAGENAKIGSKKESKEFAKDKDTKYYLVKAEKGKQGGTGGIGGNGGNAGL
                     GSKAGQVYLLGKATLLKGAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GISAPIDDSDRNYYYFDRYVFNHASDEKEVADDGVKLNALYEKHKDWTISKREDSGSV
                     GAEGKDGLSGDTLKEKELLKQEHSLSIDTFFYEFFERFGFSGSKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYGFEKKNKAVLSLDDMNIKKEFYKEA
                     KEIYEYLINKLMQSDRIESFIGEKQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKILKELEEGKKDELKEGYFNTYKESILTQIESGNQQIAVLKNELENK
                     MLSVNAKMNLLIQEIIKIKEDEKEDIEKLKEKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYQWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEGKRDKFDQIKEIIVKSIDDDNYSDKDDDIQRAKDGLSESE
                     YEEIYTKIQKKEIGLLEANYNNKQQKDKLAALEKSKQTIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLKVKSDFESKIEVIKDAIKGKEDNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELITKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSGSGSQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVVNQAKEQNRQIKEFLDQDAYQWQ
                     KTDNHILKVALAGSLDGKSRVGFENTYSIYDCNEEEVKKLLTGDEISFEVPLNQNFDA
                     IKYVTMYANIPNLRRNILDDSLKVKVHIHLYDSGYFSLYDDKKDTYYQYVFPQNYIDI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKKAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DLLTNEQKKKLESYFDKSSKYAWSESIDILKSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVNLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIID"
     gene            complement(33708..34219)
                     /locus_tag="PC523_001565"
                     /pseudo
     CDS             complement(33708..34219)
                     /locus_tag="PC523_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888383.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
     gene            34354..34542
                     /locus_tag="PC523_001566"
     CDS             34354..34542
                     /locus_tag="PC523_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475099.1"
                     /translation="MNKNDNLVIICMFIGMILGVAIGYVMGIYKGSVGTTMCYGLVFG
                     MLIGICIGAVIKNSNKKE"
     gene            complement(34712..35152)
                     /locus_tag="PC523_001567"
     CDS             complement(34712..35152)
                     /locus_tag="PC523_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF2475100.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKNGYARLCKLETYLETQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVMIQIGDKQVPAVEKMLISLTPKA
                     KEHWVTRYPCGNNGGWIRCRILEDADLLDIIQYIRAKKAPPKQN"
     gene            35487..36800
                     /locus_tag="PC523_001568"
     CDS             35487..36800
                     /locus_tag="PC523_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HDF2475101.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEENIEDIEPYWVCLPMATRTAV
                     GSYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRASESLQRYCSIVNKQYGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGIAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVRLPYGHTWKSFTYFLLDTLPENIRNNYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            36818..37354
                     /locus_tag="PC523_001569"
     CDS             36818..37354
                     /locus_tag="PC523_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDF2475102.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRIMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEIDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDVEEEN"
BASE COUNT        14344 a         4229 c         6389 g        12506 t
ORIGIN      
        1 atttgtccta gttgtaatga aactcacgat agagatataa atgcaagtat aaatatacta
       61 aaagaaggat taagactaat aacaattcaa aataaataac ttatgtaaga accgtaggaa
      121 ctatggggat ggcctgtgga gaattagtaa gacatatttt agtatgcaaa attctatgaa
      181 gcaggaaccc tgtgacttta gtcatgggag gttcaggtta ggataaaact tgaaaagcca
      241 aagacttcta aaagttttaa agagagtata gaaaaactac ataagtatga gccagatttg
      301 ataatagcag ttggagctag atttgcagag ccaattaaag aagctgctac taaatattcc
      361 aaacagcaat ttgcaatagt tgactatact tataaagttc agccagctaa tgtaatttct
      421 gtatcatttc aagataatgc tactggatat ttagcaggac ttattgcagg taaaatgact
      481 gatactggta aagtaggttt tataggaggg gttgagggtt ctagtagaga taagtatgaa
      541 tctggattta gaaaggggct taaaacttca aataaagatg ctgaattact agttaagtat
      601 tcaaatatat ttgaaaatcc tgaatcagct agaactattg caaaagaaat gagagattct
      661 gatagagatg ttgtattttc agctacagaa gatgatagca aaagagctat tcaggtggca
      721 agagaaaatg gtggtaaagt tatagctatt aataaagacc aatacgatat ggctccaaat
      781 aatgtaataa gttcaattat aaaagatgta gagcaacctg tctatgaact gataaagaat
      841 gttgtaaaag atggttttaa gggcaataag gtaatggaat ttaaaataaa agatggagaa
      901 cttggtttga cttcaaagag tagtagaaat ataccaccag atgttttgga atatataaaa
      961 aaagagtata acaaggtgaa agatacatat taattattga tgtagaagat tataaaaata
     1021 tactttatta agtttagatt aactatataa aattataaca ataagtgtgt gggcagaaaa
     1081 attctgctac acactttatt tatattcata taaaaaaatt atcaaaaaaa agaaaaagga
     1141 gtaaaaaatg gaatttacaa gagaaccatt tatactagat gacgatttgt gtctagcttt
     1201 ggtggacaaa agaattagtt atctaatgga aaatgaatta aaattaagaa atatagagat
     1261 aataaaaaca gtagaatgta aagaattata tgaaagcata aaatatcacc cagatatatg
     1321 tatttgtaat ttaggaaaag gagatataat tgttgcgccg aatgtatata atcaatacaa
     1381 agagttatta agtagatata aatttaatat aattaaggga gaagctaatt tattaaatag
     1441 atacccttat gatatacctt acaatattgc gatagttggt gattatgcta tacacaattt
     1501 taagtatact gacaaagcaa tattagatta tattgaaaat aataaactta aaaagataaa
     1561 tatagagcag ggttattcta aatgctcaat ctgtatagta gatagaacat ctataataac
     1621 atcagataaa ggtatttgga aaagtatgaa gaatacagaa atagagtgtt tattgataga
     1681 aaaaggtcac ataaatttat ttgagatgaa ttatggattt ataggtggat gtacagggtt
     1741 gatttctaag gataaactag ctttttgtgg agatgttaaa aaacatcctg actatgaacg
     1801 tataaaaagt tttgtagaaa gtaaaaacaa ggagatagtt acacttagct gtgaaaattt
     1861 actagatata ggttctatag ttcctctcat gacaagaaag gagcgataac tttggttgag
     1921 aaggataata tttcttccat gaaatttttg aggaataata atgtaaacga tagattaata
     1981 tatacgaatg atattgtaga agtagtgata gattctgcaa agcaagaagt attaaataaa
     2041 aaaatactta atggtactaa agctgaacaa agagctttaa atattgatga gatatcaacg
     2101 gaaataacta gtatgataat aagtataatg aaagagaaac ttttgaaaga atacattcta
     2161 aaatgctatg ggcgtacata tcctgaagat aaaaataata tatacatata ttcactgaac
     2221 atatttgcta aaaaagagat ttttatgaga gaaacagtat ttacgagggt gagaaattat
     2281 atagaagaaa atgattttat aaatgtagaa ggttttatta gatttagaat gagagaattt
     2341 atgaaatata tttctgctat aggtgatatt gcagttgaag agtatttaat aaagaaagac
     2401 caagatgaat ttataagagt tttaaaatac tttatagata ctcaagaaga aaagattgat
     2461 ttgcttaaag ttcatataat ggaagacaat acatttgttt tatacgataa aaatggaaat
     2521 aagatagata gcatagatga tgaagaaatt ataaatatgg taataagaga aaatttaaat
     2581 tatgaggact ttctaataag taccttgtta actctatgtc ctaaaaaaat agaaatattg
     2641 gatttattaa ataataattg ctcaaaggaa attatagata ctgtagaagc aatttttgaa
     2701 aataaagtta gtataattat ggaaaactag aatctaggtg tataagagtt agccatataa
     2761 aactaaaaaa attcttttta aatcgacaca aaagacttat aattgtatat aaagcatagt
     2821 atagctagag ctattaaata taaagaatat ctatttgatt aatgcaatat agcttgcaaa
     2881 tatacaattg ataaggagta attttataat ggataattta attaggatac tgatattagc
     2941 tgttattggt ggatttatag gttatgttac gaatgtagtt gctataagac tgatatttag
     3001 accaatagag cctattaaaa tacctatttt aaatatagaa atagtaggtc ttataccaaa
     3061 gagaagggta gaaatagcag caaatgtagg agaaataatt caagaagaat ttctttctat
     3121 ggatgaaata cttgcaaata tcattactga cgaagacaag gaagaagttg taagatacat
     3181 aaaagcaagg gtaaaaatta taatacatga gaaagtttca tttatcccag gtggtataaa
     3241 aaacatgata caagattatc taggagagat aattgagtct gaagttaaga aaagtataga
     3301 tgaattgagt aaaaatataa taaataaagc aaatgaacgt atagatatac aaaaaatggt
     3361 cgaagataag attaatgaac tagatttata cgaattggaa gagataataa taagaatagc
     3421 taagaaagaa ttaaaacata tagagttctt ggggcttgtc ttaggttttt taataggtat
     3481 agttcaaggg ttagttacta tgtttattta agtatatgat acttattgac tacatttagg
     3541 tattaattgt atttacttat ttgattgtat tacttattaa ctgtgcttaa gtattaatta
     3601 tatttatttg actgtagtac ctattaattg tatttactta tttaattgcg ttgcttatta
     3661 actgtattta agtatttact gtattactta ttaactatat taaatattga ttgtatttaa
     3721 gtatacaaaa cctaaaagag tacaataatt agaagttagt tttaataaaa attagtttta
     3781 gtatttatct agtcatatct atcatgcatg tggatatgtt ttaaaaattt taaatttatg
     3841 aaaatattat aatatcacta taaatcaaga gattgattta atataaaaat ataagaatat
     3901 tggtttacag atgattgaga tgaaaaataa tgggtaatta atatattaca ttaatgttga
     3961 ttgatgtata actatctgtt ataatgaaaa tatattaaaa atcatagtgt tgaagaggaa
     4021 gagtagagta gaaaccattc tcagagaatt tgggtctatg gctgagagcc catgtaatag
     4081 gtctatttga aaactacctt ggagctgagc tttattagca gtatttgtat ttatactata
     4141 tgtgaaagtg ctcgggtgac gaccttatag tcatcaagag ggttgtatat acttgtatac
     4201 gccaacttgg gtggaaccgc gaattaatat cgtcccaaat atatttgggg cgatttttta
     4261 ttacaaaaaa tctaaaaatt atttcaataa ggaggaaaaa acaatgatta aagttgcttt
     4321 aaaagatggc tcaataaaag aatttgaaaa tgctatttct gttatggatg tagctaaatc
     4381 cattagtgaa ggattagcta gaaatgtggt agcagcgtct gtgaacggag aggttgtagg
     4441 tctggaccac attatagata ctgattgtga cttgaattta tttaagtttg aagataaaga
     4501 aggtaaggaa gtttttagac atacatcagc acatatatta gctcaagcta taaaaagact
     4561 ttacccagaa gcaaaacttg ctataggacc aagtatagaa aatggattct actatgatat
     4621 agatttagac catagattag ttccagaaga cttagaaaaa atagaagctg aaatgaaaaa
     4681 aatagctaaa gaagatttaa aaatagagag atttgagctt cctagaaatg aagctttaga
     4741 gcttatgaaa gaacaaggag aagattataa ggtagaacta atttctgatt tacctgaaag
     4801 tgaaataata tctttctaca agcaaggtga ttttactgat ttatgtagag gaccacattt
     4861 accatctact aaaaaagtta aagctgttaa attacaaagt gtagcaggtg catattggcg
     4921 tggtgacgaa aataataaga tgcttcaaag aatatatgga acttcttttg agaaaaacaa
     4981 agacttagaa gaatatttac atttactaga agaagctaaa aagagagacc acagaaaatt
     5041 aggtaaagaa ttaggattat ttatgatacc tgaagaaggt ccaggattcc ctatgttttt
     5101 accaaaaggt atggaactta aaaatgaatt attaaaattc tggagagaaa tacacagaaa
     5161 agctggatat attgagatag aatctccaat catattaaat agaaaattat gggagacttc
     5221 aggacactgg tatcattaca aagaaaatat gtatacagtt aaaatagatg atgaggatta
     5281 tgctataaaa cctatgaact gtcctggtgg attaatttac tataactctc aactacattc
     5341 atatagagat ttcccaatga gagttgcaga gcttggtaga gttcatagac atgagttatc
     5401 tggtgcatta caaggattaa tgagagttag agcatttact caagacgatt cacatatatt
     5461 catgttacca gagcaaatta aggacgaaat aaaaggagta gctaacctaa tagatggtat
     5521 ctacaaaact tttggatttg aatataatct agaattatct acaagaccag agaactctat
     5581 gggaagcaac gaagagtggg aagcagcaga aaatggatta agagaagctt tagaagaatt
     5641 aggtttacca tatactatta atgagggtga tggagcattc tatggtccta agatagactt
     5701 ccatctaaaa gattgtttag gaagaacttg gcaatgtggt actatccaat tagatatgca
     5761 acttccaaga caatttgata atacttatat tggacaagat ggtgaaaagc atagaccagt
     5821 tatgattcat agggttgctt ttggtagtat agaaagattt ataggtatat taatagagca
     5881 ttatgctggt aaattcccag tttggttatc tccaactcaa gttaagatac ttcctatatc
     5941 tgataaattt atggattatg caaatgaagt taagaaagaa ttatttgata agggtataag
     6001 agttgagtta gatgatagag ctgaaaaaat tggatttaag ataagagaag ctcagcttga
     6061 aaaagttcca tacatgttaa tagtaggtga aaaagaagtg gctgataata atgtttctgt
     6121 tagatcaaga gataaaggtg aaataggtag tattaaatta gatgaattta tagctagtat
     6181 ttcaaaggaa attgaatcaa gagaaagtat tattcaagac tagtatacaa gatattagta
     6241 gctaagatat tattatataa gataaattta atcaatatgt tgatatatag tgattaaatt
     6301 tatgaaaaga tgagtataat tagaggtaca gttaatcata tttttatgat taatgtacct
     6361 ctagtttttt tgtatggtag atttattaat aaatatatca tagaagtgtt ttgaatctgg
     6421 aaattttaaa taaaaactta ttttttgagt gataattaag tgcttgttga taaggctatg
     6481 gctgtaaaaa tcataacctt attttcatat ttgttccatt ttcatgaaaa ttattatcca
     6541 tttaagaaaa tttcttaatc cttttgaaat atttcatttt tagtctagac ttaaatttct
     6601 ttcttcaaaa taaatgctta aatgaattaa aatttaggtt tattttctaa tcttatgagt
     6661 ctatttttca taaataatta ccttataata gaagaagtta tgttggggtt atgtgctaaa
     6721 aataacagca ttgtcaatga ttaattaata ttttatgtta tttttagatt tatcttctat
     6781 acatatatta atttatttaa ctttagttat agagagaatg tcgagtaaat acacagacat
     6841 tagagtctaa gaaggtgttt atggaacaag agtttttaaa agagatagct aattagttta
     6901 atgtcaaaaa atgataaata caaaagagga aaaagagagg tgtttaaatg atagaaaaag
     6961 taggagaaaa atctaagatt attaaaacat caaaagaata taagcgaact ataattgaag
     7021 aaaaagatag agaatggact caaaaattaa acgacatact tagtagaaat aatttttcta
     7081 atgatgaatt agacaaaatc aaggagttaa ttccaaaaga gattttgact agtgaaaatg
     7141 tcggagaagt tgtgactgaa gatggatatg ctagacctga atatgatgag gtatttaaat
     7201 cattgttcac tttaaataat gaatacggtt tacttgtaaa ttttataaat gatattctaa
     7261 aagatgctcc atatgctaac aggaatatca aaccgtttac tcaaataaag agaataatta
     7321 gacttgaaac tgacccaaca attaattaca ttggagagaa aaggcctaga cttgatattt
     7381 tggctgaaga tgaagaaaat aatcatataa atattgagat gcaaagagct tttgaaaatg
     7441 attatttgga aagagctgaa tattatctta gtcgtgtaca tggtagaaag ttagaagaag
     7501 gtaaagaata taaagaaatt ggaaaaacta ttggaataca tatactaaat catgtaaaat
     7561 ataatcacat agaagattat gtaaattgcc tgagattaac aatggatggg catccagaga
     7621 tttattctag taaaactgca ttatatttta tagagcttcc aaaaattcat aaatctgact
     7681 atatagtaaa tagaattatg ctatggggaa aacttattat caatccttct agtttagatg
     7741 taagggttat agctaagaat gattctatta ttaaaaaagc acttgataga ttaaaagaat
     7801 taggtagcaa tgaagaatat ttattaaatc tgaaaagagg tgcttatatt atgaataaaa
     7861 gtagaaattt taaagaagag atagaaagag agacagcaat tagagttgca aaagaaaaag
     7921 attataaaat aattataatg ttgaaaaaaa atggttttaa attagatgat atattaaaca
     7981 aatttaatgc ttttgactta agcgaagatg agataaaaga agtttatgaa aataattaat
     8041 gtataaataa aaagtgaact tatacaagtt cattaaatct gagtctggaa atatatatgt
     8101 gtatttttta aataaaaatt tattttaaag accataattt agaggtacag ttaatcacac
     8161 tttcatgata ctgtacctct agttttttta ttgtaaattt attaataaat ctacaataaa
     8221 agtggttttg ttgtaataat cattatatat aatgattatt ttttatagtg attaaatttt
     8281 cacttgtagt gcttaaatat aaatttgtaa cactattttt ataaatgtat aaatcactat
     8341 gacgattcaa ctcaaagtca ctgtacaaaa caccagaaac cttatatcaa taagatgtta
     8401 ttgcaccaga ttgttcaagt ctacgaaatg ctgtatacaa aatagcttct ttcaactcat
     8461 acaatccttt ggttttttca agtatagatt tattaatctc ataactatag ctgtcttttt
     8521 taagtaaatg ttttaagata cttgtttttg tatcacatct tataacatca gatgcgattg
     8581 tcatgaatat cacatccgat taaatatata atagttttat ggactttgct tatcaaggta
     8641 tatcatgaaa aagacatttt attgtaatat ttataagatt ttatatggaa ataatgtaaa
     8701 aactttataa ataatatatt ttatacctag ttatagataa atttatatag ttattattaa
     8761 aaaaaacaat aaaaagttta attcaatcta gcaaatttag tgatataatt aaaaaagaaa
     8821 agaaatatgg atttatttgg aataattttg acaatctatt tactacacgt gtagagtcat
     8881 atataattaa attatacata aaatataaaa aaataattta gtaatacgtt aggagaaagt
     8941 attatgataa agtcaagctt attttggaat attttcattg cgatatcctt tatgatagaa
     9001 tggtttcttt gtaagaaaat gctagattat acaagtgaga aaaagatttc taacaaaaaa
     9061 ataaactttt gtatagtagg tattattgtt tttgctaaca taatctattt ttttgatgca
     9121 cctctaaata taagagtaat tatatctatg atagttacta ttgcatttta taaaatttca
     9181 tatgatgtta ggtttataaa gtctgtatta atatccttaa tttattggat gttactaata
     9241 gctatagatg ctttgagtat atctttaaca gtttggctta attctataaa tgacatgagt
     9301 aatatgggga caggagatat atacagaatt gaatctataa tattaggaaa gatacttttg
     9361 atttcggctt tgtttttata tagagcctta aaaataaaat ttaatataga aataaataaa
     9421 aggacattat tttatatagt tattcctata gtcgctaata tagcatcttt ttttgtcatt
     9481 tttaaataca tatttaatct aaagtttggt aacttaatac ataatttcca attcgttgtg
     9541 atttctgttg tattacttgt atcaagcatc actttgattt taattgctaa gaaattacag
     9601 caatataata ctttagttgt agaaaataat atgattaaaa atactttgaa atgtgaaaat
     9661 gagtattatg aagatataga aaagaaatat ttaaaaacta gattactaag tcatgatatg
     9721 aaaaatcata tattgtgcat aaatgctatg attaaaaaag gtattgatgt tacttcttat
     9781 ttaaataatt tacaggtaga aatccagagt aatgacttat tatttaatac tggtaatttt
     9841 atattagatg ctatattaag tgaaaagaaa gaagtttgtg ataggaatga tatagaatta
     9901 aaaataggaa taaattttag ttgttgtgaa gctgttgaat tagtagatat atgtcatata
     9961 ttttcaaata ttttagacaa tgccattgag gcatgtaata aaattgatag tggaaataga
    10021 gtgatttgtg ttaaaggtga tattatgcaa aatttttatc taataaaagt tgaaaatact
    10081 aagacgaata agattattac taaaaatgga gatattttga cagacaaaaa aggttctctt
    10141 catggacttg gaataaaaag tgtaaaaaac tctgtaaata agtatggtgg gaatgttgta
    10201 attgaatatg atgataaaaa atttgtagtt aaaattgcta ttccacttag aaatgtgaag
    10261 aaagtggtta ataatataat ttaatatatg ggagttcaat atatataaat ttgatacgta
    10321 gaagtttaat gtaaaaattt tgtgtatagg aatttaacat acatatttat tttaatatat
    10381 attgaaggta tataattaaa tcatataatg acatttgcag tttgaaatta tttattgagt
    10441 atgagaaatt ttcaaggtca gaaagtttat gactattata tattatattg tttgtcgtat
    10501 ttattataga ttatataatg agtttaaaat attaaacttt taattttaca aaatataaaa
    10561 agaagttgca tcaaaaagat aaatgctctt taaagatgta acttctatgg tatatgtatg
    10621 aggagctata ttgccatttc tggaatcaag cttctcatag cttctatagt tgtttggata
    10681 agcttatcaa gttcccatcc aagcatttct gcaccacttc ttatgacatc tcgagagcag
    10741 ccagcagcaa atgatttatc tttaaatttc ttcttaacag attttaattc taaatcagaa
    10801 acactctttg aaggacgcat tattgcagct gcaccaatta agccagtaag ttcgtctaca
    10861 gcaaacaata ctttctccat aaattgttct ggtttgacat ctgaaccagt taatccatat
    10921 ccatgactta ttgtagaatc gattattgat ttatctaaat caagctctgt cattaattct
    10981 ctttgtttaa cacaatgttc atcaggatac atttcaaaat ctaaatcatg taaaagtcca
    11041 acgatttccc aaaattctac tctttcaggg tcgtactcct ttgcatagta tctcataact
    11101 ccagatacaa tttgagcatg tcttatatga aattcttcct tattgtattt tcttaatatt
    11161 tcccatgctt gttctaaagt tggcatgtaa ctcattttta tcactcctct agctgaatat
    11221 tttgaatttt tataaagttt attttccatt gattatatca atcaatttcc aaaacgtcaa
    11281 catactgata cacaattttg cagttaaatt cagaaattag tgaaaattaa atctttaggt
    11341 ctaaattctt tgtatctaaa gtttttcgtt tcttaatgtt atctttgata taaatgctat
    11401 aattaaggta ataaatccaa ctcctagtat aataatgtac actctatctg ataataaatc
    11461 aaaaagttta ccatacataa cctgaccaag aggcattgca attgttgttc ctgcaaaaag
    11521 aattgacatt acttttccta agagttcatt tggagttttt aattgtatga ttgttatagc
    11581 atatattgag ataagagtcg ttgtaaaaaa tattagcata gatgatacga taaaaattaa
    11641 aaacggaact ggaaatcccg tatttaatat aaatggatat actgaaaatg ccattggtat
    11701 gaacataagt gcacatatag taaaggaaat gtacaaatta gtgattttta gatattttga
    11761 gaaaatacca atactaacag cagctactag agaactaaaa gaaattgctc cctctgctag
    11821 accatataag aagttactag ctcccatagt gatttttact atatatggta taccaacgat
    11881 taacatagag cttaaaaata agtttattgc tatagctaaa aaaagtattt ttagtataaa
    11941 tctattttct ttacatatat gaacaatacc atcatttata tcaataaata tagtttttat
    12001 aatgttgctg gtttgctctt gtttgataaa aggaatctgt ataaatatct ccataattgc
    12061 agatagaaag aaacttacac aacttataac tataattgca ttaattccaa ttacaccgta
    12121 caagacacct cccaatacag gtcctgcaat atttgtaagg gatgcaactc cagctacgat
    12181 accatttgca ttcattaaat gctcattatc aactaaaaga ggtgtacttg actgaacagt
    12241 tggttgatac attgtgctta tgactgataa tatggtcata ataattccta caagtaatat
    12301 tgaaccatta ttgttgaata aaaagagtgc taagactaac actgtaatac tacttgaaaa
    12361 atcaaaaata accattaagt ttcttctgtt gaatatatca gcaatagctc caccaatagg
    12421 ggagaatatt ataattggga ttgaagatac tgctagtata gtagcaaata tttctgcttt
    12481 accagttata tcgagtatgt ataaagacaa agcaaattta agtattgatg caccaaataa
    12541 tgagattatt tgacctaata tcatcaagtt gaaattttta ttaaaagtat tcaaagattt
    12601 taccatatat atacctacct atctaagatt attaaaacat ttacaataat tgctataata
    12661 gcaattacta taaggacttt ttttcttact ttaataccta atattattag aaatatagaa
    12721 acgattatag ataacataga gtaaagtggc atgagctcac tgctagggtc tacaagtttt
    12781 gtgtggatat ttagtttatc atttattttg taaataacat catttagtct ttgtattaat
    12841 tcaatcatat tatcctttct agttataaag aaatgtttag aaaatttatt ttaaattatt
    12901 actcaagtat atgttctaca agattgtcta ccataaaatc aaatacttca atgtagtcat
    12961 atccaatatc ttcttttagc aataggcttg acagtagggc attgcaaaca ccaaaggctt
    13021 tttctttctc tatttttaac ttgagattat gtctttcaat taagctataa aaactttgta
    13081 cattgttgta ttcaagttgt gaaagcatct ctttaggtat cttattcaat aaagtgatgt
    13141 aatcagtatt atttggtgaa tataagaatg ggtttttatc atataatcga tacaacatct
    13201 ttaaggcttt tgcaaaacct tctttttgag gcatgttacc taatatttca tctactgatg
    13261 atataagtct gttttggatt ccatttagta cttcaaaaaa taacttttct tttgaatcaa
    13321 agaatgtgta gaatgtaccc tttgcaattc ctgattttga acaaagttca tcaatattag
    13381 tttttttgta tccatattta gaccagttga tttcacattc tgaaattagt ctttttctta
    13441 tattatcttt ttctatttct gtaaagcttt tagccatatt tatcaatcct ctctgtatat
    13501 acgtattatc tatgacttaa aatgtttttt aagtcataga tatatagtat tgtttattta
    13561 taaatttgtc aagtgattta tattatgaga cttgtattaa aatttgattt gaataagttt
    13621 caagcgagta tgttatcaaa atagaaaagc gtagaaaaaa cgttaactct aaaaaatttt
    13681 tctaggtaaa cgtttataat aaaaagaaat gtttacataa cgaataaatg gctataattt
    13741 aaatacacaa agttatatca gagagaggaa taaaaatatg tttaatgaac ttagaacaaa
    13801 agaaaatgta tataaaaatt taataaatgg aaaatgggta gaatctaatt caagaaaacc
    13861 aatagaaatt tactcaccaa tagataattc tcttgttggg aaagtacaat ctatgacaaa
    13921 acatgaagta gatgaggcta taaaaaatac aaaagagagt ataaaagtat gggctgatat
    13981 gcctgtatat aagagagcca atatatttca taaggctgct gatttattgt tagaaaatat
    14041 agatgaaata gctaatatac tggtacttga aatagcaaaa gacatgaaat ctgcaagggc
    14101 agaagttgag agaacagcag actttttaag atatacagct gatgtaggaa aaaatatgga
    14161 gggtgaagct attagtggag ataatttccc tggtggaaca agaaataaga tgtcttatgt
    14221 ttcaagagtt ccattaggta ctgttttagc tatatctcct tttaattatc ctgtaaattt
    14281 atcaatgtca aagattgctc cagcacttat tggtggaaat gcagtagtat taaaacctgc
    14341 tacacaaggg gctattagcg cacttcatgt ggtagaaatt atgagaaaag ctggtatacc
    14401 agatggagta cttaatacta ttacaggtag gggtagtgag ataggggatt atgttgtaac
    14461 gcataaagga attaatttta ttaactttac tggtagtact gaagttggtc agcatatatc
    14521 taaaatatct ggaatggtgc cattattatt ggaacttggt ggaaaagatg cggctatagt
    14581 attagaagat gcagacttag attttgcagc taagaatata gtgtctggag cgtattcata
    14641 ttcaggacaa agatgtacag cagtaaagag aattttagta caggagagtg tagctgataa
    14701 attagtggaa aaaataaagc cattggtaga gaatttgaca attggaaacc caaaagatga
    14761 agtagttatc acaccactta tagataataa agcaactgac tttgttcaag gtctagttga
    14821 tgacgcatta cataaaggtg caaagttgat agtaggtaat attagaaaaa ataatttatt
    14881 ctacccaaca ttgttagata atgtaaatac tgatatgaag attgcttggg aagaaccatt
    14941 tggtccagtg cttccaatta taagagttaa agatataagt caagcaattg aaattgctaa
    15001 ccaatcagag tatgggcttc aatcatcagt gtttacaagt gatattgata gggcatttta
    15061 tatagctgat aagcttgagg ttggtactgt tcaaatcaat aataaaactg aaagaggtcc
    15121 agaccatttc ccatttttag gtgtaaaggc atctggtatg ggaactcagg gagttaagta
    15181 ttcaatagaa gctatgacta gaccaaaggc agtagtggta aatgttagag gattgtaata
    15241 aatagagata aagaataata gaaataaaaa ataaaggagt tgtcttaaat ttgatttttt
    15301 gtaattgaat tttaagttca actccttttt gaatttttat atgctatttc ttagtaatta
    15361 ttttttaata aatatcttaa actttgagat tgatgaaatt tgaacatgat aaatatctta
    15421 aatttatagt cttgttttct aatgatgaag cctatttcta cttaaataga gcaatttcta
    15481 aaatgaacat ttttatctcg agtaccatat acaatgaaaa aaattaacct tgacaagtga
    15541 ccgatggtcg ctgtatgata aataaaaaag aaacgaccag tggtcgttaa aaggaggtat
    15601 cactctatgc caaaaggaat tgtgcttaca cccgaacaac aagcagaacg ccgagaaaaa
    15661 atagttgctg ttgctttgag tctcatggct gaaaatggat tccaaaaaac atcaatgaga
    15721 gaaattgcag ttttagccaa catgggaaag tcaagtctat acgatttttt caaaacgaaa
    15781 gacgagattg ttgtatacgc agttgagaaa gaaatagaaa aaactatcca acaagttcat
    15841 aggattattg atgatgaact ttcgccacaa cagtgcctta gaaaaatcat gcttaatcat
    15901 cttagatttc caaagcaata tagaactgta ctgatgtggt taaatacaga atctgattat
    15961 ttagaagaag agtatcgaaa gagacttaaa gatgctcgtt atgcatatca agatattata
    16021 aaatcggtaa ttgataatgg tgtgaccaca ggtatctttc gcaaaacgga taccgctctt
    16081 gtgacacgtt tgttaattaa ctccatgtta tcaatcagct acacgtcacg actatcggat
    16141 agttcggaaa aaatgttgga tgaaacaatg aatatatttc tgcacggaat tatgaatgat
    16201 ggaggtgaat gattatgatt tattatgttt taccactaga acataaacaa gcaaccattg
    16261 aaattgttgg tggaaaagga atgtctttat caaaactatt gacagcaggc atccctgtgc
    16321 cagacggttt tcatgttacg actgcttcat accagatttt tgttgagacg aaccatattc
    16381 agtctcgaat taataaactg ctagatggta ttgattccaa taataccagc caacttgaag
    16441 atgtatctaa gaaaataggg gtgcttttcc ataatggaga aatgccacag gaagtgtcgg
    16501 acgcaattaa aacagcttat gacggattag ggaacatatc ggtggctgtc cgttcatctg
    16561 cgactgctga agatttacct gacgcttctt ttgcaggtca gcaggaaact tatcttaaca
    16621 tacaaggtga ggatgaagtc attgacgctg taaagagatg ctgggcttcc ttatggacag
    16681 ctcgtgctat tgcttatcgt gtgaagaata atataaagca cgagattgtc gcacttgctg
    16741 ttgtagtaca aaagcttgca ttttccgatt catctggtat tatgtttacg ttaaacccaa
    16801 tcaatggtag acgtagcgaa atgattatta acgctgcatg ggggcttggt gaagcggtgg
    16861 tttctagctt agttacaccc gatacaatcg tagtggataa ggactccgaa cgaattatat
    16921 cgtatgaagt ggcaaataaa gagattatga cggttcgtac ttcagaagga acagaagaaa
    16981 ccatggttcc tgaaaggttg agaaaaaaat atactcttac tcgtaatcag gtaatgcaac
    17041 tgatacagct tggaaagaaa attgagaagt attatcaaat gcctatggat gtagagtggg
    17101 cactggaaaa agataaattg tacattgttc aggctcgtcc aattactgtt ctgccaccgg
    17161 aatgggtatt gccagaacag gatgttgtat acacaaaggg aagtttggca gaacacttac
    17221 caaatcctgt tacacctttg ttcgccacgc ttggtcttga aatcgtaaac cgtgcatcgg
    17281 agcttttatg gatagatatg tttggtaaaa gtgcgaaaaa actactgcca aaaaacgggg
    17341 catacacggt tattaatgga tatgtttatc tttctgctaa atcaaagcca tttttgattg
    17401 ctatcaaatc cctttcccca cgctctttac gtaggacact tatgaacagc gttgcacgct
    17461 gggaaacggc acgaaaagga tatgaggatg ctattaaaca atgggaagaa aagcctctgc
    17521 atacgatgaa cgcacatcaa attatggagg gtattcagac aatattttat gccgcatgta
    17581 tttatttcac aaggattcag cttacgttgc ctgctgcttc tatcagtgaa acattattta
    17641 caaaattatt tcaaggagca gttcgccgtg ctggtataac ggatacttcc gttttcctgc
    17701 ttgggtttga tacgattgct ctgcaatcgg aaaaggagtt atggggtctt tcagaatggg
    17761 taaagcaaaa taacagcatc aacctttatc tgcaaaacag tcctgcaata aagatagcag
    17821 aagatttcat gtcttcagtt gtgcctgttg gagtttcaca ggaagtatgg atagaatgga
    17881 aaaatcgaat taataagtac tttaaagagt ttggtcgtac cgcttatgaa tttgattttg
    17941 cgtattccac accacaggag acacttacac caacttttga atccgtaaaa acatttgtgg
    18001 aaggaaaagg ggaaagtcct tttttacgcc agaccacgtt tgaaaagcgt agaaaacatg
    18061 cagaagaaga gattttacaa cacataggtg gattgcgtaa aaaactattt tttaagttgc
    18121 tccattgggc gcaggaaacc tctcctatgc gtgaaaatgc tatttatttg atgggaatgg
    18181 gacatcctct gattcgctgt atgtttcagg aaatctcaga acgattcata cgaggaggag
    18241 ctatttcaca tttagatgat atttattggc tcacaaaatc ggaattggag gcgcttatag
    18301 agcagttgga taaaaataag tctctatctg atatgaacgg gttaatacct gttcgaaaag
    18361 ctgaacttaa aaaatacatg ggttatgtgt caccatccaa gttgccagag aaaaacaaaa
    18421 aaactatatc acaagcacta cagaagcaga aagatggtaa aattgtgttg acgggtattg
    18481 gtaccagtac tggtgttgtg acggctccag cttgtgtact gaatagtcca gcagattttg
    18541 aaagctttca acctggaagt gttttggttg cagttaccac aactccagca tggacaccat
    18601 tattttcatc tgcaagtgcg attgtaacgg acattggtgg tcctcttagc cattcaagca
    18661 ttgtcgcacg tgaatatggc attccagccg ttatggctac acacattgcc acacgaacta
    18721 tcaagagtgg acaaatgata acggtggacg ggttggcagg tacggtgacg tttgatgagt
    18781 aaaaagccta ttttcgcttt gaagctttca acctttgtga ttgcgttagc ggaagtagta
    18841 atacttaaag ttttgagatt taatcatact actaataccc accaaacagc ggtaagaaaa
    18901 accatagctc ggtgggtatt ttcttatgca aaaaaatgag agtgtatcaa aataaaaatt
    18961 tgattagaaa aaatcaattt tgagacaatt cctttatttg ttaatacaat aatatcactt
    19021 ataataagca attttatatt gttaattgga ggagttttga aacataagtt tttcaaaatc
    19081 ttttattggt aaaggtctag agaacacgta tccttgtaac atatcacagt ttatttcttg
    19141 caagaaatct ttttgaaaac tggtctctat tccctcagct acagtggtca tttttaactt
    19201 tttagccagt tcgattatag atacaattat atctctttca cgttgagaat ctaaatgttc
    19261 tgattgaaaa aatgctctat ctaattttaa tgtatctaca taaatatcct taagcatatt
    19321 gattgaagaa tagccacttc caaaatcatc aatagagcat ttaaagccat ttttatgaat
    19381 ttcatttatt atttcactaa atactttgac atcttcaaat acaattgttt ctgtaatctc
    19441 aatttctata aggtgagaag gaatatcata ttttttagct attttatcat atgtcttgat
    19501 gaaattattg gatgaaaaat gataacgaga catatttact gaaatattta taggatttaa
    19561 tccatcatca atccattttt ttaaagtctt gcaaacttcc tcaaaaacat ataaatccaa
    19621 ctgtataata aatccatttg tctcaaataa tggaataaat tcgtttggag gaattattcc
    19681 ttcaacagga tgaatccatc ttactaaagc ttctgcacca catatggttt gggtagataa
    19741 ctccaacttg ggttgcaaat aaacttgaaa ttctcttttt tcaagtgctt catacattct
    19801 attttctatg ctttgttcta gaataagttt ttccttatct tttttactat agaaattaca
    19861 aatacataaa ccatgatttt ctacgttttt aattgattta gaagctacat ttgcactatc
    19921 ttggatattt gttatatcta aagaagtatc ataaattaca tatgcaccag cagtaaaata
    19981 aagtatatat ttataatctg cgttatcgtt gaacctatta atatgtgtaa ccattgagtt
    20041 cagtctagaa gttatatctt ctggtttatc ttttaaaaga agaataaatt tatcagcaaa
    20101 gattctggta accaattcac cctcatgtaa gtgtttactt atacagttgt agatatattt
    20161 taaaagctta tctccagcta tatttcctgc taaatcattt attaatttaa atttttgcat
    20221 gttcaaccat attagagtat atgtacccga tggtgattga ctaataactt cttttgctat
    20281 tatattaaac tttgtatgat tcataccacc agtaacggag tcaataaaag ctaatttctc
    20341 taattcttta ttattttttt caataatctc attttgtttt actaatgttg ttacatctga
    20401 atatattaaa agatatatag gtttaccatc ttcccaatct atacattcag ctacaacttg
    20461 catatgaaca atatccccat tgatattttg tggagaaaca gaaaaacaaa cgtatttccc
    20521 agctttaaaa tcactttgat atttgtacac agcactaaga taactttcta aatttatatc
    20581 atctaataga aaatttacat tatctttgaa catgtcaaag tattttttat tagcatcaag
    20641 aagttggaat ccaaagtcag taaccttaaa cttggcaatg agacctggaa aattgttgta
    20701 agttacagtt tgttctattt taatcatcat ttgttctgta atatcaatca taacagagta
    20761 tgaaatagga tatccatcaa cgacttctcc tgttaaaaat ccaaataact ttatccataa
    20821 tttttttcca ttacggtgtt tcatacgaca gacatagtca tatttttttg ctccggtgga
    20881 gaatgtcttt tctacatact ctacaagttc attccaatcc tctggattat ccttgtaaaa
    20941 gaggtcgcat tgattgtgaa atagagtttc gtactcttct tttgtatatc cgaacatttc
    21001 ataataacgg tcattagccc aaacaacagt aaagtgttca tctaataggt gtttactaac
    21061 acttacatcc agtgcattca ttaataaact gtattcaata tttgcaaaat ctatatattt
    21121 tgacattctt agtactccct tataacatat attttgtaga atatctgaac tagctagttt
    21181 aaagtagatt tatttttaag cttattttca tacatcaaag aatctgcctc tgctattaaa
    21241 gtttcaatat cggatattcc atcccactca actaatccat agcttatgga aagagcataa
    21301 ggatactcgt cggtattact taattttgat aattcataat ttatatcaat cattattttt
    21361 tctgcttcta tacaacttgt atcctgtaaa aggaataaaa attcatctcc accatatcta
    21421 aacagtaagg aattttctac atttataagt ttttttatta tttttgcagt agtggatatg
    21481 tatttatcgc caaattggtg tccaaagtta tcatttacaa gttttaatct gtttaaatcg
    21541 gtaaaacata aagtaaattt gtagtgattt gacatccatt ctgatagttt ttgatttaca
    21601 aaaggtctat tgtagaagcc tgtcagtaaa tctgtatata caatatcatt caatttatta
    21661 atttcatgtt ctgtttttat gctttctaaa tattctagtt ggtttttaat atcataactt
    21721 tttatttgag taattctaaa actatatttt tctgcaatat agttacctac caaaactatt
    21781 atggcagact taattccata taattttaaa atcaatacat ttccaacagt ataatatatt
    21841 gatagactta aacatagata caagtacatt acaaacataa ggcttaataa aaaatttaga
    21901 ttttttttgt cctctaaaac tgtgtcaaaa ttaatctgat tatttttatg atttttataa
    21961 cagaaatata gacctgtaac tataaagcta gatgatatag aatatactcg gtaaatattt
    22021 gtaatgtaat tactgtcaaa taaatatgct ggcatatcta atgttattga cattaaagac
    22081 ctaaaaaata tgtttattgc caaaccaata atagaacagt ttagtgaata aaaaagattt
    22141 tttattatag aggttttgta agttatttta atttctatga ataaaatcaa cgtaaaaatc
    22201 gtccaattta taattagatg taaccttaaa taagtacaaa taataaaaat taaataatta
    22261 aacagagagt atataatata tactttatat ttatttttag agttactaat cattatatcg
    22321 agatatttaa aaaagttata atgataaagg taataagata aaaaacatat taaaattata
    22381 agtaatctca tatacatatc cattttagtt actgttcctt ttatgattat tttgtttatt
    22441 agtgtacatc ctaatatcag caatattaat aatttcttct acagtcattt tgttttcgct
    22501 aggaacttcc acaataccat agctaaaaga tatcttatta tacatacttt tacgtatttt
    22561 ctctataaat tcttttgtag tgttttcatt agatttaggc ataataacta taaactcatc
    22621 tcctcctatt ctggcgaata tatcatctgc ttttaaattt gaaataatta tacttgaaaa
    22681 gttaataata tagttatcac cagctaaatg tccatattta tcatttattg tctttaattt
    22741 gtctaaatca atatatatca atgaaaataa taaactatta tcaatgtaat gttgtatttt
    22801 ttcaaaagca aattttctat tatatgctga tgttaattca tcaatgtttg cttttttctt
    22861 taataaattg acttttaata attgttcttg ctttatcatt tttagtctat tatgttcttt
    22921 ttctaaataa atattttttt caatggaata tatatgattg atgaatataa tcatttgtaa
    22981 tgataataat atgttacttc ctaatataaa ataagaagtt aaagcgcttg gtggagaaaa
    23041 tgcaatttgt atactatcga ataatatata tcctaaagtg aagtagataa actttaaaaa
    23101 tagactaaat tctatttgat tttgagttat tacataaata tgctctttta ttttatccat
    23161 agagtaccat aagataaaat ctaaaataga agctattaaa atactaatta tcctagatga
    23221 catatttaca aatattatat ctggacttgt attttcgaca attgacatta tacttatagt
    23281 aataagatgt aaaatacaaa aatttattac tgctatatta ggaataaagt gtttgttatc
    23341 agaatttttt atgctaatta aaagcaattt atgacttact atatagaaca atatataaaa
    23401 tagtggtaat ggcaattttg tgaatattat tgttaagaaa gttagtggtt ttattaaaaa
    23461 aatatattta taattcttat gtatagctat acttttattt ccaaaagatg ctatcttgta
    23521 gatgtaaata ttacatagtt caactattaa tatgatagtt atgattaaaa atatgtggta
    23581 tttcatagta catcctcttt taaaatgaaa ttctatttac ttaaaataga ttagatttta
    23641 tatactctat tttacagttt ttatttaaat acatgtaaag aacttatgga tattttttta
    23701 ttgtgtatga ttgtgttaga taaagtttga aaaatatctc tatatcttac atttatacat
    23761 agtaagtatg ataattatac agtaagtatt atatgattta ttatgtaaaa taagtagagt
    23821 tattctatca ataaatatac ataatatgct aaatatatat attttttact gaaaatttga
    23881 aatacttaaa attgttccag ttaatacact acaataatat gttaacttat tgtagtatga
    23941 ctatagtatg ctattttact gcattacagt attagaaacc ttagactacg gatataaatg
    24001 gtttatatta attatagatt atgaagaaaa aataagcaac tattttgctt tattgaataa
    24061 aagtaagtat aacttacaaa aaataataaa tttattttac taaataggaa aaatataaag
    24121 aaaaaaacta aaatttgatt atttttgaac atataattaa acaaatttat attggtaaat
    24181 ttacacacag aagatagact cgattctata aaatttgctc ttaaatgaca gggaatatgc
    24241 cacaattact attgaggaga tagtactttt actatggaat aagatagatt atatagctgt
    24301 agataaaata aaaattgttt aagacgttta caaaataatt gtgaggagtg taaggtttat
    24361 ctagactttg ttttgtaaaa tagcagatta tacaagaatt tatatgtgaa taaatttagg
    24421 agtactataa aaaataataa agtttttata tgattttaaa taaattaagt taagtaatgg
    24481 atattttcta agtataataa ttgaaaatat ccattgttgt atttattata ttttaaaaat
    24541 aaaatatgtt ttgtattgta gaaaaatctt gttatctata aaattaagtt attttataag
    24601 tatttttatc cagttatacg tatttattga ccagttatac aaaaatattg aatattaaga
    24661 aaattagtac tactatataa ataaatatat atatataaag gaggtttttt atatgagtct
    24721 tattgttggt ggatggtcaa aatttagggc tgtcgaagaa gaagatttaa aagtatttaa
    24781 tgaagctgta ggaatgttaa aaggggttga ttatgaacca cttattgttg ctactcaaat
    24841 tgtagcagga acaaatttta aatttatttg taatgctact tcagttacaa atcctccgca
    24901 tgattactta gcagaaataa ttatttttaa ccctcttcct tgtgataaac aaaaagcaac
    24961 aattacaagt attaatatag ttaagtaata ttaataggag ctggaatatg agattaaata
    25021 taactaaaca aaatacgata gaggttgaag gtagtaaaaa agaagatgtt tattttataa
    25081 tagttgataa tgtttttttt gaacgcaaag gtaatgtatt ctatagtatc acaaataata
    25141 atttaaataa ttatgatata aaaatattac caactgataa tatttttcta gaattaaaaa
    25201 taacatctac taaagattta gaacctgttt ttattgtagc tgaagataga caatacaatc
    25261 attgcagtgt aatgtttaaa ccagtaatgg tgtagtgttt aatcaaataa aattaaaata
    25321 ttcaggctag taatggtgtg atatttaatc aaataaaatt aagatattca acccaatagc
    25381 aatgtaatgt ttaatttaat tgtagaaata taattttaat aagaaagtct atatttataa
    25441 atagagcaaa atagtgaata aataatataa aaacaactgt agtacataaa gtacaataaa
    25501 ataagttcat taactaaaat aactgtgttg ataaatttta tattatacaa cacagttatt
    25561 ttaatgaata gaaataaata attataaaat gaaaatataa acagattaaa aacaaaaaat
    25621 aaaaacaaat ttaaatacag tctaaaaact ttacaattta cctaagttat taaactttat
    25681 ttaaataaga atatataagc aaggaggcaa atgaatgcca gaagataata aaacattttt
    25741 atatgaatta aaagagttat taagtaacgg agaaaaacaa atagctaaag caataggtca
    25801 agatttaatt ttaattcttg gtggaacagg ctgtggaaaa agtactgcaa tcaattatct
    25861 tgcaggatgt gaaatgcata aagtagaaga tgaagatact ggagtatctt acattacctg
    25921 cgaagaccct gtagcagata ttggttcagg agctgtttct aaaactttat atccagaggt
    25981 tatagatatg aaaaaaggct ttgaaggaat gactgcaaaa ttttgcgata ctgcaggatt
    26041 tggtgataac agaggagcaa tccatgatat ttgtgcagca gtttcattag gtgaagtatt
    26101 taaagattct gcatctatat gtgcagtaat tgtcttgata ccagaagcag atatattaga
    26161 ttctagactt acaaaggtac tagacttgtt ccaacagtta aatatgtgtc ttcataaaga
    26221 aaattttaag aatagtataa cttttttcat ttcaaagagt ttcttaggaa gaacagaaaa
    26281 acaaatatat aatgttataa aaagaaaata tgaacagtta tgtgaagctt cagatagtaa
    26341 tatgtcagat tcaaatagta atataaaaga aaaagcttgg ttgtttgaag aaatgctaaa
    26401 agatgatggc aaaaatatac atatttgtaa tccgcttgca tcagatgata gggaaggctt
    26461 attaagacaa gtactagaat tagaggaagt taaagataag atatcagcat tccaatatcc
    26521 aattagtgca gatgcactat tagttttaga tacgttgata aaagaaattc aagacaatat
    26581 cgtatctagc ttagaagatt atgttaaggc ttatacagaa gattttatca gtgctgttgg
    26641 tagtataaca atgattgaag aaatagcaaa atatgatgat gtagtagaag tatttaaaaa
    26701 ctggttggaa agtacaacaa gtccaactat aggtagattt atcaacgaat tagccagaca
    26761 aagcagttta ttcttacaag atagttgcat gaaattacaa aagaatataa aagaaaattt
    26821 agatataatt gatgctttaa gtgaatatac agaacatgga actagtgaaa tcttattatc
    26881 ttctgtacat atggaattta ttatagaaaa gacaagtaag aagataatgg aacaaaactt
    26941 cattataata agaaagaatt tagaaaaagt tttagtttct tatgaagtgc aaaaacaggt
    27001 atttacatta ccaaatagag aaaatctaat ggaagttgca aataaaaagg attataaata
    27061 tagtgtaaat gattttaatt caattttgac aaatgttaca gttacacaaa aagaagagtt
    27121 agaagacagt ataacaagat taaatgcata tggagaagaa ggtaatgaaa taattagaga
    27181 atttattagt aaatacattt taaatccatt tatagtaaaa acaattaata ttaatgataa
    27241 gcaaggtatt gaaataagag cgttaattcc aaacttagta attagtagtg tattggaata
    27301 tttcaaaaat agaaaagtgg atgacaatgt ttctacattt atgttctttg ctaaaaatac
    27361 tatttatatg gatgataacc ttggaatgga ctttgcatca gagaaaaata ttattattgc
    27421 ttgtaataat ctcgacgtag gtagtaatgt aaaaattaat gtaagtggaa agagtggaga
    27481 acttgtagat aattctatgt atgggaaaaa tggtgaagat ggtagagatt caggtaatat
    27541 ttacttatat attaaagaag aaattagaaa ttatcgttta acactgattg caaatggagg
    27601 aaatggttct aaaggaatag atgggaaaaa tggtgaaact ggagcttcag gaagtaatgg
    27661 agctgatgct ggatatacaa gtagtttcca cggaaagatg tttaaagggt attttgcaca
    27721 aggtacaagt ggtggaaaag gtgaaaaagg tggcgctggt ggaaatgctg gaagaggcgg
    27781 aaatgctggt aagaaaggta atatttactt catttcagat tcagaagaac agaaaaatag
    27841 aataattaat cgttgtgata taacagtgag agatggagaa gtaggtccaa atggtagacc
    27901 aggttcaggt ggatatggag gaaatggtgg ttatcacgga tataatacac tttcattttc
    27961 tccaagtggt atagataaga ctgtatacta tacaggatac tttacaagtt gggattataa
    28021 gttagatagt ataattaaat atcatataga atttagtggt ggaagttgga atgaaggtaa
    28081 aacaggacca ggaagagaat acgctgaata tggagacaga ggagcagatg gaggatttaa
    28141 tgctccaaac actaagatat caacagctgt ttcaaaaata gaagataatt ttattaaaga
    28201 aaaatatcaa tgctattgtt ctgaaatata gaaagggata gatatatggc aaataaactt
    28261 tatagtggaa ttgtaaacct tttggaagat ggaagggacg agcttagaaa atataattta
    28321 gaaaagaagt ctattttatt atttttaggt gcttcaggtg tcggaaagag tacttgtata
    28381 aattatttaa aaggttgtgt aatggaagaa aaaacagatg cagaaactgg gcaagtatat
    28441 ataactgcaa aagattcggc tgtcgaaatt ggaaatggtg tatattctaa gactttatgc
    28501 ccagaagttg tagatattgc aaatagggat ttttcattat gtgattgccc aggttttttt
    28561 gataaccgtg gagcagagta tatgattgca ggtgctatgc ttgtaaggga aaccatctct
    28621 acatcttcaa aggtgaaagg gatggtagtt attttagact atcaatccct attagactct
    28681 agatatacat tattaataga aacgggcaaa aatttgaaag atatgcttgg tgattatgca
    28741 aagtataaag aaaatttctt ctttcttatc aataaaatac ctaaagcact aataagcaat
    28801 gtaacagaag aaaaaataag aaatatattg aataaaacaa aagaagattt agatggacaa
    28861 gagatagatt atgatggatg taaggaattg atttacttat tggatgtaat gttatcagat
    28921 agtgtatcta tagaattgtg taacccacta gataaaaatc cagaaaagtt attggataaa
    28981 atgaaaaatt tacctgaaac ttttgaaaaa acaaatctgg gattgatgct tactgcttct
    29041 tgtaaaatag agatacaaaa agctgttgat gtaacataca aagagttaga aaaactttta
    29101 gtcccttata tagaaagtgt caaaaaaggt atggaagatt ctatttccac tctatcttct
    29161 ataaatagtc taaaggataa aaaaattcaa ttagacagga tgtatacgga tttatgtggt
    29221 agagctaata gtatgaaaga gcttatggag tttgtacaag aactttcata tggtgatggt
    29281 atgcagaaag tgaatatatt agaagaaaca cgtcttcttt tagagttaaa tagctattgc
    29341 agtgagaccc atattgttgg tctaagtgag ttgttaaaat cattactaaa aaatacttta
    29401 gaagatacgt taaaagatat taaatattgg atagcagagg aagaatataa ggaagaagaa
    29461 gccaaaaagc ttgaagatgc agaatatagg atagagaaga ttcaaagttg tacatatata
    29521 aaatcatatg agaaagaaaa tgtactttct atatatattc ctatacctaa agtgaattta
    29581 tcagatttga ttaaaataat atctggttat aaacttgata ttttatcatc tatatatatg
    29641 tatggagatt gcgaattaaa aattgattgt agtatggaga aagacgtgtt ttcaggtaaa
    29701 aatattgtat tgcttacaga gaaagtattt ataccagaag gctcttattc aatcaatgta
    29761 tctggtaaga atgcggagaa gttggctgga gaagattata tatacaatgg tatgaatgga
    29821 agaaatggta ataatgcagg tacttcaggt gatattatat tatcttttaa agaaatagaa
    29881 aaaataggag aacttaaact ttatgtagat ggtggacgtg gtggaaatgg acaagatgga
    29941 aaaataggag aaacagggct aactggaaaa gctggggaaa acgctaaaat aggttctaaa
    30001 aaagagtcaa aagaatttgc taaggataaa gatacaaaat actatttggt aaaagcagaa
    30061 aaaggaaaac aaggtggaac tggaggtata ggaggtaatg gtggaaatgc aggtcttgga
    30121 agcaaggctg gacaagttta tctccttgga aaagctactt tattaaaagg tgctataagt
    30181 aagaaagatg ggaatcacgg tttagcagga aaaggaggct ctggtggtgc tggtggtgca
    30241 ggaggtcttg gtggaattag aggtatctat gaagaaacaa taagatatat aggcatctct
    30301 gctccaatag atgatagtga tagaaactac tattattttg atagatatgt gtttaatcat
    30361 gcttcagatg agaaggaagt agcagatgat ggagtgaaat taaatgctct ttatgaaaaa
    30421 cataaggact ggacaattag caaacgtgaa gattcaggtt cagtcggagc agagggaaaa
    30481 gacggtctct ctggagatac attaaaagaa aaggaactgt taaagcaaga acattctttg
    30541 tcaatagata cattctttta tgaatttttc gagagatttg gtttttctgg aagtaaaggt
    30601 tatagaaatc ttagactgtt gtgtgcagta ggaattttag aagcggatat tttagaactt
    30661 tcacatattt atgaaatttt tcatgcagtt gagaaaatag agatgcagtt aaatagagaa
    30721 atccttcaga aatactatgg ttttgagaag aaaaataaag cagtactttc tctggatgat
    30781 atgaatataa agaaagaatt ttataaagag gcaaaggaaa tatatgaata ccttataaat
    30841 aaattgatgc agtctgatag aatagaatct ttcataggag aaaagcagaa ttttagtgaa
    30901 tggagtatga ttttatttgc acttgaacaa aaaacagcat tctatgtgca gatgatagaa
    30961 gaaagtgaaa ttggaggaaa tcgtgttaca gatattcgtt ctttgacaga ttatctggat
    31021 aagatcttaa aggagttgga agaaggaaag aaagatgagt taaaagaagg gtatttcaat
    31081 acatataagg aaagtatcct aactcaaatt gaatctggaa atcaacaaat agctgtttta
    31141 aaaaatgaac ttgagaataa aatgctttct gttaatgcaa aaatgaactt gttgattcaa
    31201 gaaattataa agataaaaga agatgaaaaa gaagatatag agaaactaaa agagaaacag
    31261 gcaaaacttg aggaacaaat gaaaacacaa ttaattcttt ctggaataca aagcgcattg
    31321 agtttattga ctgcatttag tggcgttggt caagcttgtt taggagcatt tgacagtgca
    31381 aaatctttga aggaaaatct ttcagctaag gaagaggaat accaatggac taacaggatg
    31441 gttgattttt cagattttaa attggattct gatggtatga gatttgtact tggagaacag
    31501 ttaagtgaag atagaaaaaa tgctttagat aaaatgactt ataacttggc aggtgttgat
    31561 gaaggaaaaa gagataaatt tgaccaaatt aaggagatta tagttaagtc tatagatgat
    31621 gataactatt cagacaaaga tgacgatatt caaagagcga aagatgggtt aagtgaaagt
    31681 gagtatgagg aaatttacac aaaaatccag aaaaaagaaa taggtttgtt ggaagcaaat
    31741 tacaataata aacaacaaaa agacaagtta gctgcactag agaaaagtaa acaaactatt
    31801 gaaagacaac aaaagacatc taaaattaaa aatgcattga gtattggaag ttctgcttgt
    31861 tctcttataa atactgcaat tagtggtgtt aataattgtc ttaaagtaaa atctgacttt
    31921 gaaagcaaaa tagaggtaat taaagatgca attaagggta aggaagataa tattaagaaa
    31981 ctagatgaat tgaatactca aatggtggag tttaaagata aaactttgaa ggaatctgtt
    32041 ataccatttg tagaaaaatt tgatagtaca gttaaaaatc aagatatttt tgaaaatgag
    32101 ataagtaaat taaagatgaa ggaagtttta actgaattaa ttacaaagtt tggaagttta
    32161 aaaatggaaa attcagagga gatttctata atatttaaaa aagtacagga tatgatggaa
    32221 actcaaatta ttattactga taagattgat aagaagaaag ataatcttgc aatgggagaa
    32281 ttgatttatc aaatgtcagg tagtggtagt caaatgaatg aatatcagaa aaaacttatc
    32341 catcaaattg aattaaatca ggttagatat ttgtgtcagg tggaggcagc agcttttcaa
    32401 ctatggacat ttccatttgg aagcaatata gtaaacatat taaatagatg tcatgtaggg
    32461 attacagaag atgaaaacga ggttgtaaat caggcgaaag agcaaaatag acagattaaa
    32521 gagtttcttg accaagatgc ttatcaatgg caaaaaacgg ataaccatat tttgaaggta
    32581 gcattagcag gaagtttaga tgggaagtca cgtgtggggt ttgaaaatac atattctata
    32641 tatgactgta acgaagaaga agtgaaaaag cttttgacag gagatgaaat tagttttgag
    32701 gttccactaa atcaaaactt tgatgctata aaatatgtaa ctatgtatgc aaatatccca
    32761 aatcttagac gaaatatctt agatgatagt ttaaaagtaa aggtgcatat tcatttatat
    32821 gatagtggat acttctcttt atatgatgat aaaaaagata cctattatca atatgtattt
    32881 ccacaaaact atatagacat tacacatggt cttgatgtta aatacaatga ttcagattca
    32941 ataaactcaa tatggtcttc ggtggaagat aaaggaattg aaaagttcaa aaaagcagga
    33001 ttaaactctg gtagaagtcc atattctatg gctaagattt ctttgaaggc acattttaat
    33061 aagaagcttt cttatgattt acttacaaat gagcagaaga agaaactaga atcatacttt
    33121 gataaatcta gtaagtatgc atggagcgag agtatagata tattgaagag tctagggtat
    33181 attgatttga agggaatttt gtcaaagagt tggtatgaaa tcaagaaaga tatagatagt
    33241 gatggagaga ttatgaagca tttttctata ttacaagtta atttattgga aagaattatt
    33301 tatgaacaga ctagtatatt aaaggaaaat atacctaatt tgacaattga attggtaggc
    33361 tgtggtattt atgtagagtc tgaatttaat tatctaaagc aaaatgattt aaaatgctat
    33421 gaagaaagat tggttgaaaa tacgattatt gattgaaaat ataactaata gataagtttt
    33481 aaaagaattt acaaaaggag atttttcaaa atgaaaattt gaattatctc cttttttgta
    33541 aaaaatagaa agaagttgag tttaaaattt gtagaatatg ttttatataa ttttgattag
    33601 aaaaaaacta cttttaagac atcttctttt taaaggatga attataaaat aaaatagtat
    33661 ttgcacatgt tacaccttca aggaattgta taggctaata tgctttataa aaagccgtat
    33721 gaaactattg tgtttgctag atgttcaagt ttactctcgt caggagattc tcatttttcg
    33781 gatttctcaa tcatagttac tatttggaga aatctagtag catttgcttt tccttgagac
    33841 acataacaat attgtattat tctattacag tttcagaata tatgtaagag ccttcttttt
    33901 tgttgacttc aagaaattca ctgtgtcaaa ttgtatatag atttttttct aatacattct
    33961 tctaataatt agtcaatacc aataagaaac atgactgaac cagccatatt tatatccttt
    34021 ctaaaaatag aaatacctat agcagaggca tctaaaatag aaatttctaa ttttttattt
    34081 agtagagaag agagaccttt tttaaaatat tttatagagt taagtgcgat tataaaaatt
    34141 ttaagtactt tgggaaacat attttggata tacttttgat tagtagttta tttactaatt
    34201 tgtctctgta ataattgtta ttaataaatt tataaaatta tatgacattt ataccagtat
    34261 atattagaat taaagaaagt taatttatag ttctttgttt taagttaaga aaccgagcta
    34321 agcaatttaa aatatatgga atggagaaga agtatgaata aaaacgacaa tttggttatt
    34381 atatgtatgt ttataggcat gatacttgga gtggctatag gttatgttat gggtatttat
    34441 aaaggaagtg taggtaccac aatgtgttat ggactagttt ttggaatgct aattggcata
    34501 tgtatagggg ctgttatcaa aaattctaat aaaaaagaat aaataaaact ttatttacta
    34561 ggatatttag ttacaattta tttatgataa aattttttac gtatatgcaa aaaatcaaat
    34621 aaggcataat aacagatatt tttattatta tttttctaca tagaaagaat tgaatttata
    34681 tacactacat ttaaattata aaaaataaaa gttaattttg tttaggtgga gctttttttg
    34741 ctcttatata ttgaataatg tcaagtaagt ctgcatcttc taaaatacga cacctaatcc
    34801 aacctccatt attaccacaa ggataacgag tgacccagtg ttctttagct tttggtgtca
    34861 aagatattaa cattttttct actgctggaa cttgcttgtc acctatttga atcataacag
    34921 tgaatgcacc tttttcaaaa aaagcataaa ataaatgaaa agatttatgg ctatatttgt
    34981 aaccccaacc ataattattt ccaaaaggaa atcgaagttc tcttgataaa ttatattgag
    35041 tctccaagta agtttccaac ttacacaagc gtgcatatcc atttttacct atatattcta
    35101 ttatttgaga caagcaaggt ttattgcttt tatcaagcat acgctcatac attagaattt
    35161 acctccgttt ttacttttaa gtatagcaaa gtaattatga caacagtatg tcaagttttt
    35221 atgtctgatt atactaatat ttaaaaatat aaagtaatta gttttagcta atataaaaat
    35281 atgatattta tatatgttgg aaaaaaatgt aaaataatga taaaatatag tttataatac
    35341 aaaagtataa ttatattata attatatgta tttagttgga tttgtgtatt taaaaatgta
    35401 taatttatgc tgattactgc taaaaaagca taataatatt gcattgtaag attttataaa
    35461 cttctaattg aaaggggata attcaaatgg ataagagata tttagaatcc gatgtatttg
    35521 aagcttttca agaaagactt tcttatcttt tcaatgaatt tgattatatc tatgtatctt
    35581 ttagtggagg aaaagatagt ggtttactgc taaatttagt tttggattat aagagaaaga
    35641 ataatataag ttccaaaata ggagtatttc atcaagattt tgaagctcaa tacagtttga
    35701 ctactgaata tgttgaaaaa gtatttgaag agaacattga agatattgaa ccatattggg
    35761 tgtgtttacc aatggctaca agaactgctg tgggaagtta tgaaatgtat tggtatccat
    35821 gggatgatga gaaagaggaa ttatgggtaa gagaaatgcc agtcaaagaa tacataataa
    35881 atcaaaaaaa taatcctttt acttattata aatataaaat gaggcaagaa agtttggcaa
    35941 aacaatttgg tagatggatt agagatatac atgaaggtgc aaaagttgtt tgcttattag
    36001 gaacaagagc atcagaatct ctacaacgtt attgttctat tgtgaataaa cagtatggat
    36061 ataaaggaaa gtgttggata actaaggaat ttaaaaatgt ctggacagca tctcctatat
    36121 atgattggga aatagaagat atatggttgg cccactataa gtttgattat gactataata
    36181 aaatttatga cttattctat aaagctggaa tagctccaag aaaaatgaga gtggcatcac
    36241 cttttaatga ttcagctaaa gagagtttaa atttatacag aatattggac ccagatgtat
    36301 gggtgaagtt ggttgctaga gtacaaggag ttaattttac ctctatttat ggaaaaacaa
    36361 aggctatggg atatcgtagt gttaggttac catatggaca tacgtggaaa agttttacat
    36421 attttttgct tgatacctta cctgaaaata taagaaataa ctatattaaa aaattttcaa
    36481 catctattga gttttggcag aatacaggtg gtggtttgtc agaaatagtt atccgtgaat
    36541 tgttgagaaa aggttataaa atagaattaa atggagtttc aaatcataca aaagataaaa
    36601 agagtagagt tatatttttg gatgatatac ctgatgatac agatgatatc aaatttacta
    36661 aggatatacc aagctggaaa agaatgtgtt attgtatttt aaaaaatgac catatgtgta
    36721 ggtttatggg ctttggtcct acaaaagaac agagaattag aacagaatat attaagaaga
    36781 aatatgaaaa aattcattag ataggagtta agatgctatg gaatataaaa gtcctgtata
    36841 tggaatcatc gcagtgccaa tcgaaaagat agaatctaac gactataatc caaatgcagt
    36901 agccccacca gaaatgcagt tgttgtacaa aagtataaaa gaagatgggt atacaatgcc
    36961 aattgtctgt tattataatg aggaaaaaga taagtatatt attgtagatg gttttcatcg
    37021 ctatagaata atgcttgaaa atgaggatat atatgataga gaaaacggac ttcttcctgt
    37081 ttcagtaatt gataaatctc ttgatgaaag aatggcaagt actattcgac acaatcgtgc
    37141 aagaggctct cataatgtgg atttaatgag taaaattata actgaacttt gtgaaattgg
    37201 aaaatctgat gaatggatat caaagcacct gggtatggaa attgatgaaa ttcttagatt
    37261 gaagcaaata actggattag cttatttatt taaagataaa gagtttggta atgcatggat
    37321 tccttttggc tctgatgtag aagaagaaaa ctaaagatat ttattaaaag aaaaagatat
    37381 attaaaaaag aaaacctaaa atatatatta aaaaataaag agttttaata ttatataagt
    37441 acagagaaaa gagtaggaaa atgtccta
//
LOCUS       DAMJVM010000029        37416 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.029, whole genome shotgun sequence.
ACCESSION   DAMJVM010000029 DAMJVM010000000
VERSION     DAMJVM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37416
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.029"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            453..4001
                     /locus_tag="PC523_001570"
     CDS             453..4001
                     /locus_tag="PC523_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2475103.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAIFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINDKMVIDLMDSNVKRSSLMRM
                     TISTLDGECYSNDGIIRNVQDRDYFKKAKNGERNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVANKNGAIIAQKTNKNSKTLSCIFQL
                     FNKNNCDEVYCLKTIKENMKNDKSGYFNYDLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYVFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSDEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVKKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLKDELNNKLIKLIFK
                     DDRIGLINSINKQLKLSDTFASEQRVEKREGTIIWILLKGRKIIYENGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSEGSEYEEREIRLRTRDTTYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDDFKGINDNLGHLYGDAVLSEIASDLVNLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRKYKISLSVGIAKFPQDGMDYMTLFRNADIALY
                     SAKGKGKNCYTLYDKKIDKVKYIINTRSTEDIDNVEKSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNLVCKNTFEWCNEGITSEINNLKNIYYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGSEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLGMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESAPNTHVGDLCYKAIWNNQNKPCKKCPMIYLKDGDDTYKSFMRNKKF
                     EIQANITTTTLKWFDGKDACLIFGSEVTSIESEENT"
     gene            4186..6120
                     /locus_tag="PC523_001571"
     CDS             4186..6120
                     /locus_tag="PC523_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDF2475104.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLENAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHRDKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLNREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACNKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKSEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRINKNQQKSSTRKKKKH"
     gene            6226..7221
                     /locus_tag="PC523_001572"
     CDS             6226..7221
                     /locus_tag="PC523_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2475105.1"
                     /translation="MKKYISIILLVVMAMVVLVGCSPGKDNSQNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKDGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     DYSDINFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            7193..7978
                     /locus_tag="PC523_001573"
     CDS             7193..7978
                     /locus_tag="PC523_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2475106.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIILNSSFYIDILYSIGRCFFSFLLAMFFAIILSIVSYLNRFFRN
                     LLKPINALARSIPTMILVVLALIWFEKDSTPFIVGFAIVFPILYDNVLGAILNIDKSL
                     LEMASVYKIRFIDKVLKIYLPVIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7966..8664
                     /locus_tag="PC523_001574"
     CDS             7966..8664
                     /locus_tag="PC523_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2475107.1"
                     /translation="MEKIRLSIENINKRYDNRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNKVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTKLNKICDEYLELVGISEYKKFYPQMLSGGLRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFKNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSNELEKNEVLKLI"
     gene            8787..9260
                     /gene="ybaK"
                     /locus_tag="PC523_001575"
     CDS             8787..9260
                     /gene="ybaK"
                     /locus_tag="PC523_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDF2475108.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKFTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9457..10296
                     /locus_tag="PC523_001576"
     CDS             9457..10296
                     /locus_tag="PC523_001576"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HDF2475109.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLGCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEVGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTSINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            10309..10857
                     /locus_tag="PC523_001577"
     CDS             10309..10857
                     /locus_tag="PC523_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HDF2475110.1"
                     /translation="MIKITTPVSEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10923..12104
                     /locus_tag="PC523_001578"
     CDS             10923..12104
                     /locus_tag="PC523_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HDF2475111.1"
                     /translation="MSKDYAKLALEMHEINKGKISVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAVINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALESSVENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12399..12785
                     /locus_tag="PC523_001579"
     CDS             12399..12785
                     /locus_tag="PC523_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2475112.1"
                     /translation="MEYKVNDQELNASMFVPFVNRIWPGDYDMDKTQTALSKTLNITA
                     YDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQP
                     GVEAFYEKNGCQRSLQSYIIKKKDSY"
     gene            12935..13198
                     /locus_tag="PC523_001580"
     CDS             12935..13198
                     /locus_tag="PC523_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2475113.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            13719..14402
                     /locus_tag="PC523_001581"
     CDS             13719..14402
                     /locus_tag="PC523_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2475114.1"
                     /translation="MSSILLVEDDLSLIYGLEFSIQKSGFSIDIARTVKEAMQMYEEK
                     NYDLLLLDVSLPDGNGFQICEKVREVSNIPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNVASNVNELSSNNITIKLLENRVFKGEFEVELTTAEYKLLC
                     LFMKNKNIVLTRKNILDKLWDGNGSFIDDNTLSVYVRRLRNKIEDNPENPKFLLTVRR
                     MGYKWNVIK"
     gene            14413..15654
                     /locus_tag="PC523_001582"
     CDS             14413..15654
                     /locus_tag="PC523_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2475115.1"
                     /translation="MKIFSNKDIKMFFLILISILVMFIIVGQIVLISITNNYKKTLIE
                     HDYNIAGYLYSIGVDKSKIPSAFVVHEDKYILKGKEILDTAGYGFDIDVSFTPSVKLF
                     QSKYIKTMSIYLLVFFTMIIFIPYMYFFRQQSKIEEASYKIDSFMNGNINERLNTYEE
                     GSLSKLFSSIDVMSTSLNTHILKEKQSREFLKNTISDISHQLKTPLTALIMYNQILQE
                     ESHNSDVVEAFVQKSQSELERIETLIKNLLKITKIDSKTIVLNKNNVNLKNLIVKILY
                     SFETRAEKEGKVISIKGSENTLLFCDYEWMSEALSNLIKNALDNTKENDKITIEWFET
                     TITTIVSVKDTGNGIHVEDIHHIFKRFYRSKFSKDNQGLGLGLPLVKSIVEQHNGTIS
                     VESKLSQGSTFTISFLKLTNM"
     gene            15726..16409
                     /locus_tag="PC523_001583"
     CDS             15726..16409
                     /locus_tag="PC523_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2475116.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGRIFIDGKEICSMKEEKQTIFRRRNIGFIFQAYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKNHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSNDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGV
                     LTDLGVNRE"
     gene            16406..18736
                     /locus_tag="PC523_001584"
     CDS             16406..18736
                     /locus_tag="PC523_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2475117.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIIIAVCLVTAIFGLADMAI
                     QCQKIQFIKDDGNWHIVIRNIDEKFISEINSRVDIAHHGRGQVVDSGEFKSKEILIQG
                     LDEEVAKQFNLEIIRGRYPIGENETLIDEDAVKSFNLKIGDFIKIKLADGRNKNYKIV
                     GEYSSISSLRATDKHGLILSILGTQAIKKSETYDYYYITFKEKVNIRKATSDIKNTFN
                     LSDKQIGKNERLLSIIGQGDSDFSKPLYLTAGLLFVLVLVASIIMIYNSFNMSVIERV
                     KFFGLLRCLGSSKHQVKKFVVLESLILSIKAIPIGLVLGSLVTIISSIFLKYVNELFS
                     SMPILKVSFIGIAFGIIVGFLTVILSAIIPAKKASRVSPLSAVRGNLMSKEILNNGKT
                     SKVANTGSFKIETSMGLNHAFSRKKSFVLMTSSFAISIILFLGFSVLIDLMYQGLKPM
                     KQEAPHISIVDPNFKSVLTKEDINEISNIKGVKKVYSRMMTDIEATYNGKKTKSQLIS
                     YEKYQFNWAKKYLLKGEVNENKLQSENLALVEEGHGFDIGDKIYIKNGNAEKELEVGG
                     ILSSIPFEVKDKYIGNIITSENTFTNITGIKGYIIMDIQVDKDTSGDLIENIRRVVDD
                     NLEVRDKRQSNLEVKNSFYTMATFVYGFVFIIAIISMFNIINSMNISVTSRKNYYGVM
                     RAVGISNRQLRRMVIVEAGSYAVSGCLLGSILGLMLHRYMFISLVSSMFHLEWQVPFN
                     LLLITVLTMIIITLLSVRKPVKKICELDIIDSINAQ"
     gene            complement(18917..19759)
                     /locus_tag="PC523_001585"
     CDS             complement(18917..19759)
                     /locus_tag="PC523_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2475118.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVARLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            20055..20789
                     /locus_tag="PC523_001586"
     CDS             20055..20789
                     /locus_tag="PC523_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2475119.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGNPIEYTISIFRTDKYQYEIVLSE"
     gene            20799..21932
                     /gene="nagA"
                     /locus_tag="PC523_001587"
     CDS             20799..21932
                     /gene="nagA"
                     /locus_tag="PC523_001587"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDF2475120.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYEQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GDYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAISLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGEVVYKNI"
     gene            22031..22780
                     /gene="nagB"
                     /locus_tag="PC523_001588"
     CDS             22031..22780
                     /gene="nagB"
                     /locus_tag="PC523_001588"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDF2475121.1"
                     /translation="MRVLVCKDYDAMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYKTVYGEITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPNDYKLV"
     gene            22955..23227
                     /locus_tag="PC523_001589"
     CDS             22955..23227
                     /locus_tag="PC523_001589"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475122.1"
                     /translation="MLKIIILSGILSSIIKLPIIGILPTGNIKKNTGINDSKKDGKQR
                     RSNLGISIYQLPLFLYSPINNSVPSNKPIPPEEIRLIKIYLLKSCI"
     gene            23887..24591
                     /locus_tag="PC523_001590"
     CDS             23887..24591
                     /locus_tag="PC523_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2475123.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNLVNMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKVWGYDIEVETRAADDTVKRLRKKILDTNI
                     LIETVWGFGFRLKEKS"
     gene            24593..26080
                     /locus_tag="PC523_001591"
     CDS             24593..26080
                     /locus_tag="PC523_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2475124.1"
                     /translation="MKNGNDKNIIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTETVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTRENDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLVLFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSNVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKYASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEKMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKHNQKNIVVIVEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIINKHGGRIYVENGKKGAKFTILFKL"
     gene            26285..27898
                     /locus_tag="PC523_001592"
     CDS             26285..27898
                     /locus_tag="PC523_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2475125.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGLCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFIKEGFEIMFENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     FPLFLIPIFSLFLSVSVFDVSKFFSLECLKFLSLISSIGILYINFIVFYLFKFIIDKT
                     KLSMKYEILEKEIIHKEELRLSNERYKIIVEQTDSVVFEWNIKENKSFVSQAWNEKFG
                     YNNVCKNIFKEIKDKDLVHSEDNAIFENFLESIKKKNRHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELKTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQASFGSRDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(28113..29090)
                     /locus_tag="PC523_001593"
     CDS             complement(28113..29090)
                     /locus_tag="PC523_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2475126.1"
                     /translation="MIQCEIADRNSDKKINTLEVIIFFVVTYGISLLFGIPLYFKKYM
                     NPQIMVTFMTILPASGVAITKIYKHKKSDEHIILHCVIIADFLLSLFLVTLKAFGVID
                     DETILLGMGILVLFTGIITICYACLEGKELYPFKNVNKSIICIFIFIIIINLSKCIML
                     GWKDINFINLIMNIFLIFPYIANQSILFFGEEYGWRGFLQEKMQTKFGKRMGVILLGI
                     IWELWHIPLWFTVYHVDVLGILLRLFSVISLSIFFGYVYMKTKNVWSCVVIHLINNTV
                     LLDTDLNNAFLNFNASSLNSSIILGCLIFSVLLSLFIFSKEYKKEQLRY"
     gene            complement(29107..29937)
                     /locus_tag="PC523_001594"
     CDS             complement(29107..29937)
                     /locus_tag="PC523_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2475127.1"
                     /translation="MSQVPKNYFTTGEFAKICGVNKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLKEIKMPLKEIKTYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTITLKEHEEEYLVKTPITYKEQFLGDEEENFLYECI
                     NFIDNYELSDYGMIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKQKGLYVTA
                     YHKGSYETIYKTYEKLLNFFSQNNLCIGDFVYEEYLLYDISVRDSNEYLTQISAEVKI
                     "
     gene            30010..31737
                     /locus_tag="PC523_001595"
     CDS             30010..31737
                     /locus_tag="PC523_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2475128.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGI
                     VKGDIDYIVKTGLMMIIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKVMTIIIV
                     VVMFLFMLSAFLVGKRLIRLFKIMQIKMDNMNRVLREVVTGVRVIRAFNRSHFEKKRF
                     DKTAIDYSETAISINKVFSVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNVYKDDVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISL
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            31775..33637
                     /locus_tag="PC523_001596"
     CDS             31775..33637
                     /locus_tag="PC523_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2475129.1"
                     /translation="MSEKRTDKQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLVSAIFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRRAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPRGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHEELIEKKGFYEELYNSQFSSEYDEDVV"
     gene            33968..34657
                     /locus_tag="PC523_001597"
     CDS             33968..34657
                     /locus_tag="PC523_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HDF2475130.1"
                     /translation="MDKKLNYRFEMKHKITEADVLALKSRLYPIMKKDKNALKDGKYL
                     IRSLYFDTPEDKALLDKLNGVALREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSASL
                     TKTEVIKILNNDIEFLKESTNCLLREFYLKIKCERLEAKSIVDYDREAFVYPVGNVRV
                     TIDSDIKTSVNSIDLFNKHLPTVSIIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            34744..35430
                     /locus_tag="PC523_001598"
     CDS             34744..35430
                     /locus_tag="PC523_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HDF2475131.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKAFTKYQIKSKSVTPEKGIELVFEIRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            35447..37312
                     /locus_tag="PC523_001599"
     CDS             35447..37312
                     /locus_tag="PC523_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HDF2475132.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGIYSTSGNKGVDLYSDVYIEKYF
                     NRDKVMEVNIEIAESDLKDMNENAIKEEFKVAKVTIDGDTYGNVGIRTKGNSSLTSVV
                     NSDSNRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLSTPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGDVTGDLYKSDEGSSLQYKGDDPENYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLYNLIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDRNNSNNNSGNKSDEKEVTAELTLSEVKTNMDNETENNTTG
                     DSKNKNNTNDDKSGNGNNQKPEGSMGKGGKSIPGVLEVAKDMSKTIKAQLNGETSSTK
                     QNSGDESSSENKGTEKFDEDVGGMPEPPEGMDGKMPPGMGNMDNGDMNGKNGNMNRNQ
                     GNPREAGGFENKGGGSMSKTRTYSKLILGGASMIIMLIMLVGVSNINRRRFIKSK"
BASE COUNT        14743 a         3947 c         6470 g        12256 t
ORIGIN      
        1 ttactagaat tattatctgg taaactaagt tttgagtgtg aatctaaagt atatgttttt
       61 atagggctta aattttatgt atacaaaatt aatttgctaa ctgaatcata taaaaagaca
      121 agatattttt tagagggagt gattagagag tatttttttg ttgtcaaatt aactgaagag
      181 taattaatta gaaaaggaat gaataataat gaatatatta cattattact tttgataaat
      241 caaaaataag gtcagagttt ttaatattaa ttaagaatga tgaaatatga acaaagattt
      301 tacaaataaa ccttaagcta tgttatttaa ttctaatgcg ggaagaaggt aaatgtatat
      361 tgaaaaaatc atagaaataa aaataaacaa cttaaataaa tacaaaggct tgcaaaataa
      421 gtaatataat tatatacaat gaggatgtga gtatgatgaa cagactaaag ccaagaattt
      481 taggtattag tatcttgttt attattataa ttattttaac atgtggagct attttcaagt
      541 ttaaatttaa attagataat aagttaaaag aaaccacaca tacatacttg tctgagttta
      601 cagaacacaa tataattaat ctcaagacaa gacttagtgg tcaatttgac atgcttgaat
      661 ccattgctaa tttcatagga aacttgaata atataaatga taaaatggtg attgatttaa
      721 tggactctaa tgtaaagcga agttctttaa tgagaatgac tatctccaca ttagatggag
      781 aatgttattc taatgatgga attattcgta atgttcaaga tagagactat tttaaaaaag
      841 ctaaaaatgg agaaaggaat atatcagagc ctttaaaatc tattgttaat aataaagaaa
      901 taatagtttt atctactcca gtttataaaa gtgggaaaat agttggtgtt ttaagtggaa
      961 tttatgattc aaaaaagtta aatgaattat taggattatc tgcatttgat gaaaaagcag
     1021 atgtttttgt tgcaaataaa aatggagcta taattgctca aaaaacaaat aaaaatagta
     1081 agacactatc gtgtatattt cagctcttta acaaaaataa ttgtgatgaa gtctattgtc
     1141 taaaaactat aaaagaaaat atgaaaaatg ataaatctgg atattttaat tatgacttag
     1201 ataatgaaaa atttatgtta aactatcaac cacttggagt taatgattgg tatgtttttt
     1261 ctgttgtgcc aaataaaata gtgtctattc agtcagagaa tatatcttat gatgtatatg
     1321 tatttgcatt agaaatacta ttagttatat tgatttttat aatatatata gcatgtatta
     1381 taaataataa caccaaaatt attataaatg aaaaagaaaa atttaaagta ctcactgata
     1441 atatactagg aggagttaaa acctgtttaa aagatgaata tatgactatg acatatgtaa
     1501 gtcagggatt tttagaactt acaggttact ctgatgaaga tttaaataaa atatttaaaa
     1561 atagatttaa agatatgatt tacaaagatg atttagacaa gtttaattgt attattctaa
     1621 atcaattaga aaaacaagat aaaattgaag ttgaatatag aattgtaaaa aaagatggaa
     1681 gtataatttg gattttggag agaagcaagt ctataaatga taataaagga agtgagtgta
     1741 ttcaaggagt tttaactgat ataacagaac ttaagaaaat acaagaagag ttagattcaa
     1801 aaagatttga aatacaggcc atcaatgata gtattgttgg tgggatagtg ataactgaga
     1861 taaatgatga ttttgatagt atatatttaa atgaaggtta tttaaatatg attggatata
     1921 cacgtaaaca actgaaggat gagctaaata ataaattaat aaaacttatt tttaaagatg
     1981 atagaatagg gctaattaat agtataaaca aacaactcaa attaagtgat acatttgcat
     2041 ctgaacaaag agttgaaaaa agggagggta ctattatatg gattttatta aaaggaagaa
     2101 agataattta tgaaaatgga aaatctgtac tttgttgtgt tattgtagat attacttctg
     2161 ctaaattatt agagcaagaa cttaaaaaga gtgaagaacg ccatgaaatc attctaaagc
     2221 agacgcaaaa tattataata gaatgggata tagaaaaaga caaaatgaat atatcaccca
     2281 attggtataa taagtttaaa tatgacccta taactgaaaa tgcaacaaaa aatataatta
     2341 aatcagtaga tatatatcat gatgatatgg ataagtttat ttcactaatt aaacttactt
     2401 cagaaggtag tgaatatgaa gagcgtgaaa ttagacttag aactagagat actacctatg
     2461 catggtttag cataaaggca acaaccttat ttgataataa tggtaaacct tataaggtag
     2521 taggaagttt gactgatatt gatgaatcaa aacgagaagc tgaaaagtta aaagaaaggg
     2581 cagaaaaaga ccctcttaca gatttgtata ataaaaaagc atcacaatca cttatagaag
     2641 aatacataac attagataaa aaaagaaaag gtgctttaat gattattgat attgatgact
     2701 ttaaaggaat aaatgataat ttaggacatt tatatgggga tgctgtatta agtgaaattg
     2761 caagtgacct agtaaatcta tttagagcaa ccgatatagt aggtagaatt ggtggagatg
     2821 agtttattgt atttataaaa gatatttctg aaacaagaga tattataaat aaagcagaag
     2881 aactggtaaa aacatttgaa aggtcttttt taggtaaagg acgaaaatat aaaatatcat
     2941 taagtgtagg aattgctaag tttccacaag atggtatgga ctatatgaca ctatttagaa
     3001 atgcggatat agctctttac tcagccaaag ggaaaggtaa aaattgttat acattgtatg
     3061 ataaaaaaat tgataaagtt aaatatataa ttaatacaag aagtacagaa gatattgaca
     3121 atgtagagaa gtcttttaaa gaacatatta caaattatat atttgatata ctatatgaat
     3181 caagtgatat agaagtggca gtaaatttaa ttctaagtat tgtaggtaaa cactttaatg
     3241 tgagtcgtat gtatatattt gaaaattcag atgataatct agtatgtaaa aatacatttg
     3301 aatggtgtaa cgaaggtata acttctgaaa ttaataatct taaaaatatt tattacaagg
     3361 atttaggtaa ctatctttta aactttaatg cagatgggct attttattgt aatgatattg
     3421 aaaatttagg gagtgatgtg aaaaaagtac ttaaaccaca aggtatacgt tcaatattac
     3481 agtgtaaaat tttagagggc agtgaattta gagggttcat aggatttgat gaatgttcat
     3541 ataatcgtat gtggactaat gatgaagtga atacattgac ctttatatct aaaacactcg
     3601 gcatgttttt attaaaaatg cgtattcaaa gtaagctaga agagtcttta acgattcaaa
     3661 agtctgtaat ggataatatg gacatatggg catatgttgt tgacaggaat acctatgaat
     3721 tattatttat caacaaaaaa atgcttgagt cagcaccaaa cactcatgta ggtgatttgt
     3781 gttataaagc gatatggaat aatcaaaata aaccttgtaa aaaatgtcct atgatatatt
     3841 taaaagatgg tgatgatact tataaatcat ttatgcgtaa taaaaagttt gagatacagg
     3901 caaatataac tacaactact ttgaaatggt ttgatggtaa agatgcatgt ttaatatttg
     3961 gaagcgaagt aactagtatt gaatctgaag aaaatactta aaatactgtg ttatatatat
     4021 acttaaaatt gcaatttgaa tttattagac ttaaaatgtc tatataaatc atttaagttg
     4081 tggttttaag tattttttat gatataatta agcgttaaga acaaaattaa aagatacttt
     4141 tgtataaaaa aatatttgtt aaatatatat tgagcaggtg ataatatgga aaataagttt
     4201 ttaccaataa gcaaacaaga tatgatagat agaggatggg aagaattaga ttttgttcta
     4261 gttacaggag atgcttatgt agaccatcat agttttggaa cagcaataat atctagagtt
     4321 ctagaaaatg ctgggtataa agttggaata atagctcaac cagactggag aactacagat
     4381 gattttatga agctaggaaa acctagactt gcatttctag ttaatgcagg aaatatggat
     4441 tcaatggtta atcattattc tgtaagtaaa aagcatagag acaaggacat gtattctcca
     4501 ggtgggaaaa tgggatatag accagataga gctactatag tatattgtaa taaaataaga
     4561 gaagcataca gtgatgtcgc aatagtaata ggtggaattg aagcgagttt aagaagattt
     4621 gcacactatg attattggag tgataaggtc agaaaatcta tgttaataga tagtggagca
     4681 gatttattag tttatggtat gagtgaaaag cagattgtag aagtagccaa tgcacttaat
     4741 gatggatatg acccgaaata tattagacat attgatggga catgctatat ttcagatact
     4801 ttggaagaaa tatatgataa atatatatta attccatctt ataaagaaat atgtgaagat
     4861 aaaatgaagt atgtagaagc ttttaaaatt caatatgatg agcaagaccc gtttagagga
     4921 aatataattg ttcagcctca cggaagtaag tatctggttc aaaataaacc agagaaacca
     4981 cttaacagag aagaattaga tgaggtatat ggattgccat atcaaaagac ataccaccct
     5041 gtatatgaaa aatttggtgg tataccagct atagaggaag taaaatttag tatagtaagt
     5101 tcaagaggat gctttggtag ttgttcgttt tgtgcaataa catttcatca aggtagggca
     5161 gtacaaagta gaagtgagaa atcaattatt gatgaagcaa taggaataac caatttagat
     5221 gattttaaag ggtatataca tgatgttgga ggtccaacag ctaattttag aagaccagca
     5281 tgtaacaaac aaattactaa aggtgcttgt aaaaatagac aatgtttatc tccatcacca
     5341 tgtaaaaatc tagatgcaga ccacagtgaa tacctacatt tacttagagc tgtaagaaaa
     5401 ttacctaaaa taaaaaaggt atttgtgcgt tctggaatta gatacgatta tgtaatggca
     5461 gataaaaaca ataaatttct tagagaattg atagagcatc atgtaagtgg tcagttaaag
     5521 gtagctccag aacatatatc agaagaagtt ttaaagtata tgcagaaacc agcaggaaaa
     5581 acctatgata aatttagaca gaagtttttt gctataaatg aagaattagg taaaaagcaa
     5641 tatttgatac cttatttaat gtctagtcat cctggttcta ccttaaatag tgctatagag
     5701 ttagcagagt atttaagaga tactcactat caaccagagc aggttcagga tttttatcca
     5761 actccaggga ctctatctac aaccatgttt tatacaggta ttgacccact tacaatgaag
     5821 ccagtttatg ttcccaaatc aaaacgtgac aaggcaatgc aaagggcttt attacaatat
     5881 agagcaccta gaaattatga tttggtatat tctgcattag ttgaagcagg aagagaagac
     5941 ttaattggtt ttggacatag atgccttata aagccaaaaa gtgagaagcc ttattttaat
     6001 agaaataatt ctaaaaaaaa tgtatctaaa ggaacaaata aaaacaaaaa aactaataca
     6061 aataacagaa ttaataaaaa tcaacaaaaa agttcaacta gaaaaaagaa aaagcattaa
     6121 agtgtaaata aaaatactta aaaaagaagg attattcgat aaattaacag aaattagtat
     6181 aaataaatat aattatttta aaggcgttac ctttgggagg aagagatgaa aaagtatatt
     6241 tctataatat tattagtggt gatggctatg gttgttttgg taggatgttc accaggaaaa
     6301 gataattctc aaaataagga gttatcagtt gtcaaacaag tgaaagtagc tgttccagat
     6361 gggctaccag cagtggcaat agccaaactt gcaaatgaaa atccagagat taaagatgga
     6421 tatgaaacag tgtatagcat tgaaaagaca cctgaggcga tttccactag agtgatgaaa
     6481 aaagatgcag atatagctat agttccttca aacatggctg cgatagctta taataaaact
     6541 tcatcatata atatagttgg aactgtggga atgggttcac tttatttggt atcaacagaa
     6601 aatataaaag actattctga tttagttggt aaagaagttg gatgtactgg aaaaggactt
     6661 actccagata taactataaa aagccttttg ttacaaaagg atatagatta ttcagatata
     6721 aattttaatt atgtaaattc agcaagtgaa ttagtaccat tattggctac tggtaaagtt
     6781 aaaacaggta ttgttccaga acctgcactt agtgctttga tgtctaaaaa tccagatata
     6841 aaaattataa aaagtttaaa tgactcatgg aaggaagtaa gtggctcaaa agatggttat
     6901 cctcaatcca ctttaattgt aaaatctgag tttttgagag ataataaaga ttttgttgat
     6961 tcttttgtag gtcaattatc aaatagtata gattgggcaa ataaaaatcc tgaagaatta
     7021 ggtgcatatt ctgaaaaaat aaaaatatct actgaatcaa agataatagg aaaatctctt
     7081 gaaagagcta atttaaaata tattccagtt aaagacatga ttaaagacta taaaaattac
     7141 tatgagaaat tagctaattt tgatgataag acattaggag gcaaagtacc agatgaagca
     7201 atatactttg ttgagaaata aaaaaaataa agaacaattt atagataaaa tggaggtaac
     7261 actttcttgt gttatcctct tatttttatg gcaaattatt gctttaaaaa ttgataatga
     7321 tattttttta ccaacagtag gtcaagtatt taatagcata aaagaaatta tactaaattc
     7381 tagtttctat atagatatac tttactctat agggagatgt ttttttagtt ttttactggc
     7441 tatgtttttt gccattatac tttcaatagt gtcttattta aatagatttt ttagaaattt
     7501 attaaaacca atcaatgctt tggcacgttc aataccaact atgatattgg tagtacttgc
     7561 acttatttgg tttgaaaagg atagtactcc ttttatagtt ggatttgcta tcgttttccc
     7621 tatattatat gataacgttt tgggagctat attgaatata gacaagagct tactagaaat
     7681 ggctagtgtg tataaaataa ggtttataga taaggtatta aaaatttatt taccagttat
     7741 aaaatttcaa attatatcta tactagtatc gacattctct ttaagtttaa aagttgctat
     7801 agcaggagaa gtatatagtc aacctacata tggtattgga actatgatac agactgaaaa
     7861 aattaacttc aatacatctg gaatatttgc atggataatt attgttcttt taatttctgc
     7921 cattttgcaa atagctcaaa agtttttagc aaggagagct tttttatgga aaagataagg
     7981 ttaagtattg agaatataaa taaaagatat gataatcgta ttatatttag agattttaat
     8041 attgattttt atgtaaatga ggtaaattgt atccttggaa aatctggttg tggaaaaact
     8101 acactcttga atataataag tgggattatt aaaaatgata ccaacaatct aaatatcaaa
     8161 gaaaatttaa ataaagtagg aaataaactt gaagctagtt acatatttca agatgataga
     8221 cttatagact ggttaacagt agaagaaaat ataaagatag ttgtaaataa atactataat
     8281 aaaactaaat taaataaaat ttgtgatgaa tacttagaat tggttggaat tagtgagtat
     8341 aaaaaattct atcctcagat gcttagtgga ggtcttagac aaagggtaaa tattgctaga
     8401 gcttttatat atccatccaa aaacattata atggatgaac cttttaaatc tattgatgca
     8461 aagaatacac agcttataat ggataatttc aaaaatatat taagaaaaga gaagagaact
     8521 gtgttatttg taactcataa tattgaggaa gcattgtttt tagcagatag gatatttatt
     8581 ttaggagatt ctccaattag aataaaaaaa atattaaaaa attctaatga attggaaaaa
     8641 aatgaagttt tgaagttgat atagagttat aataatacta tcgttttaaa acatatttgt
     8701 ttaaaatata taagactctg ttaatatggt acagaatatt caaaaatgtg tataatgttc
     8761 gtattttata aaaaggaagt gaagatatgg ttaagactaa tgctatgaga atattagatt
     8821 caaataagat tgattataaa gtaatgtctt atgaagtgaa aagtgaacat gtggatggtg
     8881 ttgaggtggc acatgatata ggaagagatg taaatgaagt gtataagaca ttggtaactc
     8941 aaggagttag taagaatata tatgtatatg taataccagt acatgaaaac ttagatttaa
     9001 aaaaagctgc aaaagtagct aaagagaaaa gtgtagagat gattcatgta aaagatataa
     9061 ataagtttac aggttatatt agaggtggat gttctcctat aggcatgaaa aagctttata
     9121 aaacttttgt aaatgaaagt gcaaagaatt tagatacaat tattgtgagt gcaggaaaaa
     9181 taggatatca aatagaacta tctccatttg atttacaaag acttataaaa gtagaatttg
     9241 tagatgtaat aaaaaaataa gaatcacata taaaatatag agattatatt cttaataaca
     9301 ggtgttgtag tgttaataaa atgaataata attttaaaat aaatattcaa ttacttatgt
     9361 gtaattgata taaaaatatt gagaaatttt aaaaacaatg gtaaagtgta agtgtaatca
     9421 cgaatatttt ttataaagta atggagggat gttcgcttga gaaaaataaa atctgaacaa
     9481 atagtcgaac aagtaaaaaa actttgtata gaagcgagcc tatatttagg agaagatgtt
     9541 ttaggttgta taaaagagaa agctaaaagc gaaaagagtg aagttggaaa aaatatatta
     9601 aatatattag ttgaaaatgc tgaaatagct aaagaaaaaa atataccaat atgccaagat
     9661 actggtatgg ctgtattttt tgttgaagta ggacaagaag ttttaattga aggagatacg
     9721 cttactgatg cgataaatga gggagtaaga caaggctatg aagaaggcta cttaagaaaa
     9781 tctgttgtaa gcccaattaa tagagtaaat acaaaagata atacacctgc tgttattcat
     9841 tatgatatgg taaaaggtga taaaattaaa atagaatttg cagccaaggg gtttggaagc
     9901 gaaaatatga gtaagatgaa aatgttaaaa ccatcagatg gactagaagg aattaaaaaa
     9961 ttcataatag atacagtttc agaagctgga cctaacccat gccctcctat ggtaattgga
    10021 gttggtatag gtgggactgt agacaaatgt gctcaaatag ctaaaaaggc actttttaga
    10081 gaactaggtg aatttaataa ggatgaaaat atagcaaagc ttgaaagtga actattaaca
    10141 tctattaata aacttggtat agggcctcaa ggtttaggtg gtactacaac agctttggga
    10201 ttaaacatag agacttttcc tacacacata gcaggtctac ctgttgttgt aaatataaac
    10261 tgccatgcat caagacataa gaaggtagta atttaggagg aaaataaaat gataaaaata
    10321 actacacctg taagtgagat tgatatagca aaattaaatt gtggagatac aattagcttg
    10381 agtggtatac tttatacagc aagagatgct gctcataaaa gattgataga ctgtataaat
    10441 aaaggtgaag aattaccatt tgacgtatat ggtcaaggaa tatattatgt aggacctact
    10501 cctacaaagc caggagaagt tataggtgct gctggaccaa cgacaagcta tagaatggat
    10561 gatttgacta tacctctttt agagagaggt cttaggttga tgataggtaa gggaaaaaga
    10621 agtgacgaag ttatagaagg tatgcaaaaa tatggagcag tttatcttgc tgcaataggt
    10681 ggagctggag catatatttc aaattcaata aaatcatgtg aaattattgc atatgaagat
    10741 ttaggagccg aagctataag aaagattgta gtagaagacc ttaaacttac agtagccata
    10801 gatagctatg gtaataatat atatgaacaa ggtcgagcaa tttatgaatg taaataaagt
    10861 ttagaaattt atatgaatat taaaattaaa aatagttgtg tatattttaa ggaggaaaat
    10921 tgatgtctaa ggattatgca aaattggcat tagaaatgca tgaaatcaac aaaggaaaaa
    10981 taagtgtaga aagcaaggta gaagttaaaa ctaaggacga tttatcaaca gcatatacac
    11041 caggtgttgc agaaccttgt ttaaaaatac atgaaaatca agatgatgtt tatagataca
    11101 cttctaaagg gaatctagta gcagtagtta gtgatggaag tgctgtactt ggattaggta
    11161 acattggtgc tgaagcatct atacctgtta tggaaggaaa ggccatatta tttaaacaat
    11221 ttgcagatgt agacgctttt ccaatatgtt taaagactaa tgatgtagat gagatagtta
    11281 aatctgtaga gttgatggaa cctgtatttg gaggtataaa tttggaagac atatcttctc
    11341 caagatgttt tgagatagaa gagaggctaa aaaaatcttt aagtatacct gtttttcatg
    11401 atgaccaaca tgggacagca ataatagttg cagcagctgt aataaattca ataaagttaa
    11461 ttgaaaacaa aaaaatagaa gatttagaaa tagttataaa cggagcaggg gctgctggta
    11521 tagcaatagc aaagatacta cttaatatga atgttaagaa tataatctta tgtgatagaa
    11581 gtggtgcatt ggaatctagt gtagaaaatc taaactatgt tcaaaaagaa atgttaaaag
    11641 taacaaatat aagaaatgaa aaaggacctt taaaagatat cataaaagga aaagatgtat
    11701 ttattggtgt ttcaggtcca ggagctgtta caaaagaaat ggttgaaagt atgtctgaaa
    11761 agccaataat attggcaatg gcaaatccta ctccagagat aatgccagaa gaagcaaaat
    11821 taggtggagc atttataatg ggaacaggtc gttcagactt ccctaatcag gttaataatg
    11881 tcttggcttt tcctggaata tttagaggtg cactagatgt caaggctaaa gagataaatg
    11941 aagagatgaa gatagctgct gcatatgcaa tagctaatac tataagtgat gaagaaatat
    12001 gcccagagta tatattgcca gacgtattta ataagaatgt tgtaaaaaat gttgctaagg
    12061 cagttaaaga agcagcgata aaaaccaaag taaatagaat ataaattttt atatgttcat
    12121 atatccttat cttatcgggt atatgggcat ttttattata ggtttatact catatatcct
    12181 tacttttata tacatatctt tatgattaaa ggtgttagaa aatgtagtaa attttatact
    12241 atcagactct atctttatta ctttagtaga tacatggttg taaaggttta tagaaaaaaa
    12301 tataggaagg cagattttta ttgttgcgta tatatgagaa tgatataata tgaatattat
    12361 aaattaataa atttaaattt atggaggtac aagagatgat ggaatataaa gttaatgacc
    12421 aagaacttaa tgcttcaatg tttgtcccat tcgtcaacag gatatggcca ggagattatg
    12481 atatggataa aacacagact gcattatcaa aaactttgaa tataactgct tatgatgata
    12541 aagtgcttgt aggatgtctg cgagttcttt cagatggtta ttattttgga acaattacag
    12601 aattgcttgt tcttccagag tatcagaaac aaggcattgg tagtaagctt ttccagcttg
    12661 ctaaagagaa caccccaact atgctatatt ttggtgcaca gccaggagta gaagcgttct
    12721 atgaaaaaaa tgggtgccaa agaagtctgc aatcctacat aataaaaaag aaagatagtt
    12781 attaaagttc cagctttcaa taatcttaaa agtacaatat tttagtggaa tcatttcatt
    12841 ttttattact aatatgacat aaacatgtca tattagtatt gatatacttt taatatggtt
    12901 aagagccaaa gaaaaatata aggagtgatt taaaatggaa gaaactaaat tttgccaatg
    12961 ctgtgcaatg ccaatgggac aaactgatga attatatggg acaaataaag atggaagtaa
    13021 aagtactgat tattgtagtt attgttttaa agatggtgag tttacagctg atataagtat
    13081 ggaagagatg atagaagttt gtatccctca tatgcttcaa agtaataagg atatgactga
    13141 agatgaagca agaacaatga tgaacaattt ctttccaaca ttaaaacgct ggaaataatt
    13201 ttatgcaaaa ttagttatat gcaaaacatg aaatatggtg ttgagaataa ggggtggtgt
    13261 gctttatagc ataccacctg ttttgttact tgctagttca gttattcttg tatgcttaaa
    13321 tatgttctca tccctctttc tatttcaaat tgtgaaaatc taaaatatgt tatttatatt
    13381 ctacttctat atccatgtct ttttatacct caagtcaaat ccaactacaa aatcattatt
    13441 ttcaattctc tgtttaacta ttaacagata aagaattata aaattttcta ttttttatat
    13501 aaaataatac aggtcaagct gatttctagc tatttgagat agaaagaggg atgagagtta
    13561 taggagtaat accaatcatt agactgttga tttgtgtagt cttttgtaaa aacatttgtt
    13621 ttaatatatt gagtttttga aaaagtaaaa ttataattaa aataaattaa gtgtaataat
    13681 gtattagaag taaaaataat taaaatagag gtgattaaat gagtagtata ttgttagttg
    13741 aagatgattt aagcttgata tatggattag agttctctat acaaaaaagt ggatttagta
    13801 tagacatagc aaggacagta aaagaagcta tgcaaatgta tgaggaaaaa aattatgact
    13861 tattgctttt agatgtatcc ctacctgatg gtaatggatt tcagatttgc gaaaaagtac
    13921 gagaggtttc taatataccc attatatttt tgactgcttc agatgaagag gtaaatgtag
    13981 tgatggggct tgatatgggt ggcgacgatt atataacaaa accttttaaa ttaaatgaat
    14041 taatttcaag aataaaagca ttacttagac gttataatgt tgcaagtaat gtgaatgaat
    14101 taagttcaaa taatattact attaaattac ttgaaaatcg tgtttttaaa ggtgaatttg
    14161 aggtggaact taccacagca gaatataaat tattgtgctt atttatgaaa aataaaaata
    14221 ttgtacttac tagaaaaaac atattggaca agttatggga tggaaatggt agctttatag
    14281 atgacaatac attatcagta tatgtaagac gattgagaaa taaaattgaa gataatcctg
    14341 aaaaccctaa gtttctattg accgttagaa gaatgggtta taaatggaat gtaattaagt
    14401 gagggtatta atatgaagat attttctaat aaagatataa aaatgttttt tttaattctt
    14461 atatctattt tagtaatgtt tattatagta gggcaaatag tgcttataag tataactaac
    14521 aattataaaa aaacgttgat agaacatgat tataatatag ctggatactt atatagtata
    14581 ggtgttgata aatctaaaat accatcagca tttgttgtac atgaagataa atatatttta
    14641 aaaggaaaag aaatactaga tacagctgga tatggatttg atatagatgt tagctttaca
    14701 ccaagtgtaa aattgtttca aagtaaatat ataaaaacta tgtctatcta tttattagta
    14761 ttttttacta tgattatttt cataccatat atgtattttt ttaggcaaca aagtaagata
    14821 gaagaggcta gttataaaat tgattcattt atgaatggaa atataaatga aagactaaat
    14881 acatatgaag aaggtagctt atcaaaatta tttagttcaa tagatgttat gtcgacatct
    14941 ttaaatacac atatattaaa agaaaaacag agcagagaat ttcttaaaaa tacaatttcg
    15001 gatatatcgc atcaattaaa aactccactt acagctttaa taatgtacaa tcagatacta
    15061 caagaagaaa gtcataatag tgatgttgtt gaagcttttg tacaaaaaag tcaaagtgaa
    15121 ctagaacgca ttgaaacatt gattaagaat ttactaaaga ttacaaagat agattcaaaa
    15181 acaatagttc tcaataaaaa taatgttaat cttaaaaatt taattgtcaa aattttgtat
    15241 agctttgaaa ctagggcaga aaaagaagga aaggttattt ctataaaggg ttcagaaaat
    15301 acattgttgt tctgcgatta tgaatggatg tcagaggctt taagtaatct tataaagaat
    15361 gcattagata atacaaaaga gaatgataaa ataacgatag agtggtttga gacgactata
    15421 accaccattg tttcagttaa agacactgga aatggaatcc atgttgagga tattcatcat
    15481 atatttaaaa ggttttatag aagtaagttt tcaaaagata atcaaggttt aggactggga
    15541 ttaccactag ttaaatctat tgtggaacaa cataatggta ctatttctgt agaaagtaaa
    15601 ctatcacaag gaagtacatt caccataagt tttttaaagc ttactaatat gtaagataaa
    15661 atacatcttg cagtaaggta tatagattat tattatatta atcaattaga aaggtgagag
    15721 agaaaatgga tattcttgtg gttaataatt tatgtaaaac atatggaaat ggatatacaa
    15781 aagtagaggc attgaaagat gtatccttta cagtttcaaa aggagagttt gtgactgtag
    15841 ttggagaatc tggttcagga aagagtacac tattaaatct cttaggaggt cttgatactc
    15901 caacatcagg gagaatattt attgatggta aagaaatatg ttcaatgaaa gaggaaaaac
    15961 agactatttt tagacgtcgt aacatagggt ttatatttca ggcatataac cttataccag
    16021 aactaactgt ggagcaaaat ataatattcc cattgttatt agattataaa aaaccagata
    16081 ctatttactt agatgaagta ttgactattt taggtcttaa agataggaaa aatcatcttc
    16141 caaatcaatt atctggtggt caacaacaga gagttgcaat tggaagggct ttgattacaa
    16201 agccaagtat tatactggca gatgagccaa cgggaaatct tgacagtaaa aatagtaatg
    16261 atgtaatttc acttttaaaa gtgactgcag aaaagtataa tcaaaccatt attatgataa
    16321 ctcataatac gaatatagct tctacagcag atagaatact acaggtttct gatggtgtgc
    16381 ttacagacct gggggtgaat agagaatgag aagctactta gaattagtac cacaatatgc
    16441 aaaagtacat aagaaaaaaa atagaactac aattttatgt attattattg cagtatgctt
    16501 agttactgct atctttggac ttgcagatat ggctatacaa tgtcaaaaga tacaatttat
    16561 taaagatgat ggaaattggc acatagtaat tagaaatatt gatgaaaagt ttatatcaga
    16621 aataaatagt agagtagata tagctcacca tggtaggggg caagtcgttg acagtggaga
    16681 gtttaaatca aaggagattt tgatacaagg tttagatgaa gaagtagcaa aacaatttaa
    16741 cttagaaata ataaggggtc gttatcctat tggggagaat gagactttaa tagatgagga
    16801 tgcagttaaa agctttaatc taaaaatagg agattttatc aaaataaaat tagcagatgg
    16861 tagaaataaa aattataaga tagtaggtga atattcatca atatcaagct taagagctac
    16921 agataaacat ggattaatac tttcaatatt gggtacacaa gctattaaaa aatcagaaac
    16981 gtacgattat tattatatta catttaaaga aaaggttaat attagaaaag ccacttctga
    17041 tattaaaaat accttcaatt taagtgataa acaaatcggt aaaaatgaaa gattactttc
    17101 tattataggt caaggtgact ctgacttttc taaaccactg tatttaacag caggattact
    17161 ttttgtactt gttttagtag caagtattat catgatatat aatagtttta acatgagtgt
    17221 aattgagaga gttaaatttt ttggtttgtt acgttgtcta gggtcatcaa aacatcaagt
    17281 taaaaaattt gttgttttag aaagtttaat tttaagtata aaagctattc caataggctt
    17341 agtattagga agtcttgtaa ctattatatc ttctatattt cttaagtatg ttaatgagtt
    17401 attcagctca atgccaattt taaaagttag ttttattggt attgcatttg gaataattgt
    17461 agggttttta actgtaatat tatcagctat tattccagca aaaaaagctt cccgtgtatc
    17521 tccacttagc gctgtacgtg ggaatctaat gtccaaagaa attttaaata acggaaagac
    17581 aagtaaagtt gcaaatacag gaagttttaa aattgagact tctatgggat taaatcatgc
    17641 tttttcaaga aagaaatctt ttgtacttat gacttcttca tttgccatta gtattatact
    17701 atttttaggt tttagtgtgc ttatagacct tatgtatcaa ggacttaagc ctatgaagca
    17761 agaagctcct catatatcta ttgttgaccc taattttaag tctgttttaa ctaaggaaga
    17821 tattaatgaa ataagtaata tcaaaggagt aaagaaagta tacagtagaa tgatgacgga
    17881 tatagaagct acatataatg ggaagaagac aaaatcacaa ttaatttctt atgaaaagta
    17941 tcaatttaat tgggcaaaaa aatatctatt aaaaggagaa gttaatgaaa ataaactaca
    18001 gtcagaaaac cttgcattag tagaagaggg gcatggattt gatataggag ataagatata
    18061 tattaaaaat ggaaatgctg aaaaagagtt agaggtagga ggtatacttt cctcaattcc
    18121 atttgaggtt aaagataaat atattggaaa tattataaca tctgaaaata cttttacgaa
    18181 tataacaggt ataaagggtt atataataat ggacattcag gttgataaag atacttctgg
    18241 agatttaatt gaaaatataa gacgtgtagt agatgataac ttagaggtca gagataaaag
    18301 acaaagtaac ttagaggtta aaaattcgtt ttatactatg gcaacatttg tatatggatt
    18361 tgtgtttatt attgcgatta taagcatgtt taatatcata aatagcatga atataagtgt
    18421 tacaagtaga aagaattact atggtgttat gcgtgcagta ggcatatcca acagacaatt
    18481 aagaagaatg gtaatagtag aggcaggttc atatgcagtg agtggttgtt tattaggtag
    18541 tatactagga ttaatgttac ataggtatat gtttatatcg ttagtaagct caatgtttca
    18601 tttagagtgg caagtacctt ttaatttact acttatcact gtactaacta tgataataat
    18661 aactttatta tcagtaagaa aaccagtcaa aaagatttgt gaattagata taatagactc
    18721 aataaatgca caataaatat agctattgat ttaaattaaa aaaacaaagt aaaaatgaaa
    18781 tagaaaatat ttttcatttt tacttcgttt tttatattgt aaaatttttg ttatgttaca
    18841 aaatttctat aaagatgatg tgaatctaaa cattatatga caacgtgagc atttaagtta
    18901 aaatatttta aatatactat tttagataag ttccttcttc atcttgataa attaatcctt
    18961 ttttcataag cactccaaga gctcgtttaa aatttttctt acttgaatta aataatatag
    19021 aaatatcttt agggtcagac ttatcattaa atctcatata tccatcagaa ccctctaaat
    19081 aacttaaaat tttattttct aaagtatcaa gttcatcctt tctatttcct ctaggagaaa
    19141 gaccaagttt tccatcttca tatattttta tgacatgtaa attttcaaga acatcaccag
    19201 tttttagttc agtaaagtac tcattatgaa gtattacacc agcatatttg ttgtctacac
    19261 atatcattgc agaattatta gtttggaatc catatacaac tccagttaca atatctccta
    19321 cattgtaagt atggtctgtt aataagtatg aatctatatc tgtagtagca gcaattcttt
    19381 tgcttttgtc taaatatata taaaataaat atttttcgtc tctaaatagt ggataagtct
    19441 tagctttaaa aggaactagt atatctttag gaagtcccat atctataaaa gtaccaatgt
    19501 ctgtattatc aactactttt aagcgtgcta catccccaac cttagctaaa ggcggtatta
    19561 aagttgcagc agttctgtca tcagaatctt taaatatgaa agcatttact tcatcaccta
    19621 tgtttatttc attttttccg attaatctat tatgaagaag tatatcatct tttgtgttat
    19681 tagtttgtcc atccaaaaaa tatccaaatt cagtttttct ttttactgta agtttattaa
    19741 aatcacctat gtttatcaaa ttaaaatcat cctctcattt tatgtttata atttagccat
    19801 aatatactta tgtagtagtt tgtcacagta tctatattag tattagtgaa atttacaata
    19861 aataatatag ttatttatgt atttttcaat tctctagctt cttatatagt ataaactcca
    19921 atttcttttt tgtctttagg ttttactaaa atttttacaa aataaattaa aaagtattga
    19981 aataaaattt cttttaaagt ataattggta tataccaaaa ttggtatata ccatattgaa
    20041 aagtggtgat taatatgaaa atagtaaata aatctagtaa cataccattg catactcaat
    20101 taagttcaat aataagagag atgatagaaa ctggtgagct taaagaaggt gatgcaataa
    20161 tgccagaaag agaactttgt aacatacaaa atgtaagtcg tatgacagta aataaaacta
    20221 ttgttggatt agtaacagaa ggacttcttt atagggtaca aggtaaaggg acattcgttg
    20281 caaagcaaaa aaagaagtat cagttttcaa atgtaaaagg ttttactgat gttatgaaag
    20341 aaaagggagt aaacataaaa acagatatat tatcctttga gatggaacta ccagatgatt
    20401 tagttaaaag aaagcttgga ataagtgata atactacaaa tatatataag attgtcagac
    20461 ttagatatac tgacggagag ccatttggat tagaaatagt atatctatca gaagaaatgt
    20521 gtaagggtct aacaaaaggg atattggata attcttcatt atatagggtt ttaaatgaaa
    20581 aatatggcta taagatacaa aaagctgaac aggtaatgga gccagtaata ctttcagatg
    20641 aagaaagtaa gcttcttgaa acagatgaag gtgctttagc tcttaaactt catagaaatt
    20701 cctataatag agaaggtaac cctatagaat ataccatatc aatatttaga acagataaat
    20761 atcaatatga aatagtattg agtgagtagg tgaataatat gaaatgttta attaatggaa
    20821 agataatatt aaaaaatcaa atcttggaaa ataaagtact agtatttgat gaaaaaataa
    20881 ttgatatagc tgatagtgta ccaaaagatt gtgaagttat agatgctgat ggaaaatata
    20941 tatctccagg gcttattgat attcatatac atggaaacat gggaaaagat acaatggatt
    21001 caactgatga atctattgaa acaatttcaa agtcaataat gagacatgga gtaacatctt
    21061 ttcttccaac tacaatgact atggataaag aacatgttta tgatgcactt gaagttatta
    21121 aaaaagccca aaatagaaag cttgaagggg cacaagtatt aggagcacat ttagaaggac
    21181 cttttataaa tgagaattat aagggagcac aaaatgaaaa gtttataata aactcaaaat
    21241 acgaatttat aaaagaatat aaagatgtta taaaagttat aacttatgct ccagaaaaag
    21301 atattgattt tgattttact agagaaataa aaagatgtac agatatagtt ttatctattg
    21361 gtcatagtaa tgcaaattat gagcaagcta aagaagctat aaatttaggt gtaacaaatg
    21421 taactcatat gttcaatgca atgactggtt taaaccacag agacccaggc gttgttggtg
    21481 cagcacttac aactaatgtg tacagtgaac taatagcaga tacaatccat ataaataaag
    21541 atttatttca atttatacta aacaataaag gtaaagaaag attgatttta ataacagatt
    21601 ctatagaagc tggtggtctt gaagatggag attattctct tggaggtcag aaagtcatag
    21661 ttaaggggaa tgaagctaga cttgaaaatg gagcattagc tggaagtgtg ttgtctttaa
    21721 ataaaatggt atttaacttc ttagataata caaaccttaa ggtgaatgaa gcgattagtt
    21781 tagcatcttt aaatccagca acatctttgg gaataaatga caaaaaagga agcttagaaa
    21841 taggcaagga tgcagatata gctgtatttg atgagaattt agattgtaaa atgacacttt
    21901 gcttggggga agtagtatat aaaaatatat agtatacaga acaaacttta aagacagtaa
    21961 aagcaatata aaaaataata aattaaactt accttagttg aaatagtagt taaaatttag
    22021 gaggaacaag atgagagtat tagtgtgtaa agattatgat gcaatgagta aaaaagcagc
    22081 tgagatgata gcagctcaaa tagttttaaa accaaattca atattaggac tagctacagg
    22141 aagtacacca gtaggtatgt atagagattt ggtaaaaaaa tacaatgata atatagtaga
    22201 tttttcagat gtgatgagct ttaatttgga tgaatattat aagttaccaa tatctaatga
    22261 ccaaagttat gattatttta tgaaagagaa tctatttaat catgtaaaca taaagccaga
    22321 aaatactcat ttaccaaatg gaatggctga tgatattgaa aaagagtgta tgaactatga
    22381 agcttctata gatgcagctg gtggtataga tgtacaagta cttggaatag gtcgtaatgc
    22441 tcatataggt tttaatgaac ctgatactaa atttgcaaaa agaactcatg tagttgaact
    22501 tacagaatct acaatagaag ctaatgcaag attctttaaa tcaagagaag atgttcctaa
    22561 aaaggctgta agtatgggaa taggttcaat attaaaatct aagaaaatat tacttttagc
    22621 ttcaggagaa gaaaaagctg atgcggtata taagactgtt tatggagaga taactccaga
    22681 agttcctggt tctatacttc aacttcataa ggatactata gttatagtag atgaagcagc
    22741 agcaagtaaa ttgaatccaa acgattataa attagtataa taatagaatg tagtttaagt
    22801 aaaaaatagc tatcattgaa taatcaaatt tatttaatga tagctattat tatttaaatc
    22861 acttactttt tttcatttaa attagttatt attgtttaaa ttacttgttt attacttttt
    22921 acgaaaactc gttatcaaga agtatccaaa taagatgtta aaaattatta tactcagtgg
    22981 gattctatca agtataataa aattacctat aatcggaata ttaccaactg gaaacataaa
    23041 gaagaataca ggcatcaatg atagtaaaaa agatggaaaa caaagaagaa gtaatcttgg
    23101 gatatcaata taccaactac cacttttttt gtattcacca atcaataatt cagttcctag
    23161 taataaacca ataccaccag aggaaattag gttaataaaa atatatcttt taaagtcttg
    23221 catataacca gtgtgaaatc ggaaataaaa atcataattt aatttaacag ctaagaaata
    23281 cataagcaat agaataatta aaaataaaaa atatctaaga gtttttttca ttttaaattc
    23341 tcccttcaga aatactttat ccagttttat tttgttggaa tatgatttta tcatttagtt
    23401 taagaaatcc ataatattag tgaagaatga aaaaagtact atatatgaat aaatattttt
    23461 agaattaagg tgaactgtag tacttaatat aactataata ttaattgttt gaatattcaa
    23521 taaaaagttt gaaaaatatc aattttgata taaaatgaat aaatacagtt gaatattgaa
    23581 ttattatttt atcaaaaata tgcaaagtac tacactttca aatgtcacct aaagtaataa
    23641 aaggtaatta aagtgaagta gttcaagata tttctaaaat gagtttaata aattctataa
    23701 taatttattt tttgccttaa aattgcctta aaatccaaac aattttaaaa ctaatgtact
    23761 ataaaaagct ggtataattt aaatatagta aaaaataaga tttatcttat ctattgggat
    23821 tcaattattt aatcaatata tttatactat ttaatttaaa taaaaagtct attaaggagt
    23881 gagcttatga gtaaattaat ttatatagca gatgatgaag ataatataag aaatttggta
    23941 aagacattct tgaaaaatga aggtcatgat gttatggatt ttaaaacggg tgatgaactt
    24001 ttagaacaat ttaatataaa agagtgtgac ttagtaatac tagatataat gatgcctggt
    24061 tcaagtggtt ttgaagtatg tacaaaactt cgtgagaaaa gtacagtccc aataatcatg
    24121 cttactgcaa gagatacaga tatagattat attacaggga taaccttagg tagtgatgat
    24181 tattttacaa aaccatttag cccaatgtct ttggttatga gagtaaaatc tatatttaga
    24241 aggatagagt ttgaaaaaaa acaaaattat gataaatata gtaacttggt aaatatggaa
    24301 ttaaaatttg gggatgtaat aataaataaa aagaataaaa tcgttacaag taaaaatgta
    24361 aatatagacc ttacaccaaa tgaatataac ctattgactt atttgtttga aaatatagat
    24421 agagcagttt caagagatga attattaaac aaagtatggg gatacgatat agaagttgag
    24481 acaagggctg ctgatgatac agtgaagaga ttacgtaaaa aaattttaga tacaaatatt
    24541 ttaatagaaa ctgtatgggg gtttggattt aggttgaagg agaaaagtta agatgaagaa
    24601 tggaaatgac aagaatataa ttggaaagat taaatccaaa tttaaattaa agttctcaaa
    24661 gaaaaatata agcataaaaa ctaggatttt gttgagcata ctttgtttga taattatagt
    24721 atttatgata atattagtat cttttaatgt atttcttgat acatatataa aaacaactgc
    24781 gaatgaagaa ttaacaaagt ctacagaaac agtagaacat atggatagta attttagacc
    24841 tattaagcca ccacaagatg atggaaagct tcctcccaaa gtattatcta attttatgag
    24901 aaatgtacaa gataaagtta ggatggcaga aacacaatca gatgctgatg ctatggtagt
    24961 tgattctaag tataacctga tatttccaac aagagagaat gatttcttaa aaaatgttga
    25021 tgagatggag ctgattaggg agtgtattca aaatgaaaaa ttagatttaa attcagatga
    25081 aaatacacgg atatctacca gaaataagga ttattatatt tcaactgtaa aaattactag
    25141 tatatatggt gaacaagaca agtatcttgt actttttata gacatatcaa aaactctaaa
    25201 tctagcacaa aagataaata ttgtattaat atctgtaatg tgttttgctg gaatattagc
    25261 aatatttaca gctgtgattt tatctgagaa aattgctaaa ccgataaaag agttgtgtga
    25321 atttgcaaaa aaaatgggtc aaggagattt caaaagaact tactttgatt tttcagataa
    25381 agaattagta gagctttcaa atgttatgaa taagagtgct gagtatctgg ataagtatga
    25441 taatgagcaa aaaatatttt ttcaaaatgc atctcatgaa ttaaggacgc cactcatgtc
    25501 tattaaaggt tatgcagaag ctataaaata taatgttata gatagtaagt atgcaagtga
    25561 tataattctt gaagaaagtg ataggttaag tgatatggtt gaagatttat tatacatttc
    25621 caaaatagat aatataacta aagattttga acttgtagaa tgtgatttaa gagaagtact
    25681 ttcaaattgt ggagctaggc aaaatgtaag agctataaac aagggtataa aatttattta
    25741 tgaatttgat gaaaaaatgg ttttatttga atgtgatgaa aaaaatattt ccaaagcatt
    25801 tatgaatctt atagaaaatg ctttgagata cgctaagagt gaaataaaaa tagtctgtaa
    25861 gcataaccaa aaaaatatag ttgtgatagt agaagatgat ggaattggaa ttaagaaaga
    25921 agatttgcct catgtttttg aaagatttta taagggagta gggggaaatc atggaattgg
    25981 attatcaata gttaaatcta ttataaataa gcatggtgga cgtatatacg tagaaaatgg
    26041 caaaaaagga gctaagttta ctatattatt taaattgtaa aaaaattaga gaaaatatag
    26101 agatgtttga agttgttttt tatatatatc tgttgtatac tgttgaaaaa agaacaatat
    26161 taagctgaca tatattataa taattgacat ttaaagtaaa tgcttaaata tagatacgaa
    26221 aggaaaagta aaatctgaag tgcatttagg tttaagtaaa cttcttattt tataaaaaat
    26281 atgtatgaaa gaacagctga tttttaatat tacaaattta gtaacagttg tatttgaagc
    26341 ttttgtcatc catatgtttt tatcagactt tttaggtcag aaagaagggt atataaaagt
    26401 tgtaagatat gctaaattgg gttttataat atgtttagga ctttgtaatt taataacttt
    26461 aaaccctaaa attacaatgc ctttaatttt cattcttatt ttttcaacat catttttata
    26521 taaaggaaat ttaaagacac gattgtttac gactgtttta ttaagtatat tttttatact
    26581 gtcagaaatt gtagttacaa gtatttttgt tttatttata aaagaaggct ttgaaataat
    26641 gtttgaaaat aatagtataa gagtattggc gacaatttta tctaaaattg tctttttgtt
    26701 gacttgtaaa attatttgtc tattcaaaaa agatgtacat ttagatatgc ccataaagta
    26761 ttggtttcct ttatttttaa tacctatatt tagtttattt ctgtctgttt ctgtctttga
    26821 tgtaagtaaa ttttttagtc ttgaatgttt aaaatttctg agcttaatat ctagtattgg
    26881 tattttgtat attaatttta ttgtctttta tttatttaaa tttattatag ataaaactaa
    26941 actatctatg aaatatgaaa tacttgaaaa agaaataatt cataaggaag aattaagact
    27001 tagtaatgag cgttataaaa ttattgttga acagaccgat tctgttgttt ttgaatggaa
    27061 tatcaaagaa aacaaatcgt ttgtatcaca ggcttggaat gaaaaatttg gatataataa
    27121 tgtatgtaag aatattttta aagaaattaa agataaagac ttagttcatt ctgaagataa
    27181 tgctatattt gaaaattttc ttgaaagtat taaaaaaaag aatagacaca atcaagcagt
    27241 atatcgttta aaaaaatcta atggtgaata tatttggtgt agaacatcaa ttacaagtat
    27301 ttataatgat gaaaatgagc ttttaagagt tgtcggagta attgtagatg tagatagtga
    27361 tataaaaaaa tatgaagagt taaaaactag agcagaaagt gattcattga ccaatatata
    27421 caataaaggt acatttgaaa aattggtaga agaaactata gttatgaata caggtgataa
    27481 aaaagatgca ttattcatca ttgatttaga tgattttaaa gaaataaatg ataattttgg
    27541 tcatccattt ggtgattttg tactgaaaac ctttgctgat aagatacagg ctagttttgg
    27601 tagtagagac ttagtcggaa gaattggtgg agatgaattt gtagtatata tgcaagatta
    27661 cgttacagaa gtaaatttgc acaaaaaagc caaagagtta aatagagtac tttcggataa
    27721 ttacactgat ttgagtttta gttttgatgc ttctgtcagt ataggtattg caaggtatcc
    27781 tcaagatgga acttcatttt ttgaattgtt taaaaatgca gatagagcac tttatagcat
    27841 aaaagcatca ggtaaaaatt catattgttt atttgaagaa gagctatatg tacagtgaat
    27901 ttagatttta tgtgatatca agatttatat atgcttgtgg catgtctaat ttataaaata
    27961 tgttactatg tatgttttta tatgtttcag tgaggttaat taatataagc aaaggtgtaa
    28021 gtaaatttat attaatgtaa gtttatttac acctttttaa aaataaattt tgtaaattta
    28081 aacagttaat tttataatta tttttattta ttttaatatc ttagctgttc ctttttgtat
    28141 tccttagaga aaataaacaa tgaaaggagt acagaaaaaa ttaaacatcc taatataata
    28201 cttgaattaa gacttgaagc attaaaattt aaaaatgcat tatttaaatc ggtatccaaa
    28261 agaacagtat tattaattaa atgaattact acacatgacc atacgttttt agttttcata
    28321 tagacataac caaagaatat agaaagacta atcacagaaa ataatctaag taatatacct
    28381 aaaacatcta catgataaac tgtaaaccat aatggtatgt gccataattc ccatataatc
    28441 cctaataata taacgcccat tcgtttgcca aattttgttt gcattttttc ttgaagaaaa
    28501 cctctccacc cgtattcttc tccaaagaaa aggatacttt gatttgctat atatggaaaa
    28561 attaaaaata tattcataat caaatttata aaattaatat ctttccatcc taacattata
    28621 catttactta gattaattat tattataaaa ataaaaatac aaataataga tttatttaca
    28681 ttcttaaatg ggtaaagctc tttgccttct aaacatgcat aacatatcgt tataattcca
    28741 gtaaataaga ctaaaatacc cattcctaag agtatagttt catcatctat tactccaaat
    28801 gcttttaaag taactagaaa tagagataat aaaaagtcag ctattataac acagtgaagt
    28861 ataatatgtt catcactctt tttatgttta taaatctttg ttattgcaac tcctgatgct
    28921 ggtaatatag tcataaatgt taccattatt tgtggattca tatatttttt aaaatataga
    28981 ggtatcccaa atagaagtga tataccataa gttactacaa aaaatattat aacttctaaa
    29041 gtatttattt ttttatcact atttctatca gctatttcgc attgtatcat aataatttat
    29101 ctcctttcag attttaactt ctgctgagat ttgagttaga tattcattag aatctcgcac
    29161 acttatatca tatagaagat attcttcata aacaaaatcc ccaatacata aattattttg
    29221 actaaaaaaa tttaataact tttcataagt cttgtaaata gtttcataac tacctttatg
    29281 ataagcagtt acataaagcc ctttttgctt gattgataca ggatatttct tgtactcttt
    29341 attaactttt gtaaataaat atgaatatga ttcaaaatct ttattaatta tatcttcacc
    29401 tttaatgatt gagccaatca taccataatc actcaattca tagttgtcta taaagttaat
    29461 acattcatat aaaaaatttt cttcctcatc tcctaaaaac tgttctttat atgtaatggg
    29521 cgttttgacc aagtactctt cttcatgttc ttttaaagta atagtatttg catctatatt
    29581 acaggcattt ttagtaaatg aaatagtaga ttccattaga gcttgaatat tatttagttt
    29641 ttcaatttca tttttaatat caatagtttt cttttctaaa agttcaatta gtaaattagg
    29701 tgttcttttg tctatataag ttttaatttc ttttaaaggc atttttattt ccttaagtga
    29761 agaaattata ccaaaaatgc taagctgatg gtatgagtag tatctatatc cattttcttt
    29821 ctttaattct ggagagaaaa gacctatatc atcataatgg aaaagtgttt ttttattgac
    29881 tccacaaatc tttgcaaatt ctccagttgt aaaataattt tttggaactt gactcataat
    29941 tataattcct ccttgactat cctgttacag tatagtttat cataatattt tgtaaaggag
    30001 tgtgattgta tgaaaaaatt aattcatttt ttaaaaccat atagagtttt aattgttgta
    30061 gttttgatat ttacgttttt acaaacactt ggaactttgt atataccaac acttacagca
    30121 aatatagtaa acaatggcat agtaaaggga gatatagatt atattgtaaa aacaggttta
    30181 atgatgatta tagtagctgg tataacagct ttatcagctg tgttagtttg taaagtatca
    30241 gcaaatcttt catctggatt ttgtagagat attagagaag cagtatttat aaaatcacaa
    30301 gatttatcaa taaatgattt taataatata ggtacagcat caatgattac taggagtact
    30361 agtgacatta ctttaatagg tcagtctgta tttatgttca ttcaattagt attgccagca
    30421 ccaattataa ctgtttctgg cctgttctta gcttattcta tagataaagt aatgacaatt
    30481 attattgttg ttgtaatgtt tctattcatg ttatcagcat ttttagttgg taaaaggctt
    30541 attagattgt ttaagattat gcaaataaag atggataata tgaatagggt attgcgtgag
    30601 gtagtaactg gagtaagagt tataagagct tttaatagaa gtcattttga aaaaaagaga
    30661 tttgataaaa ctgcaataga ttattctgaa acagcaattt caataaataa agtattttca
    30721 gtacttatgc ctattgtaat gttaataatg aatttaggta tagtaagtat tatttggttt
    30781 ggtggtatga gagtcagtaa tggaaatatg gagataggtc atataatggc acttgttgaa
    30841 tattgtatat taattttatt ctacttaatc atgggagtaa tggtatttat gtacatacct
    30901 agagcaggtg cctgtgcaga tagagtaaat caaatattag atatagaacc tgaaattgta
    30961 gatggaaatg tctacaaaga tgatgtctca gaaagaggac atttagtatt taaaaatgta
    31021 accttttcat atgctcaatc agaagaacct gttttaaata atataacatt tgaagcaaag
    31081 tctggagaag taactgcaat tataggaagc actggctcag gcaaatctac tattgcaaat
    31141 ataataccac gtttttttga aattcaatct ggagaaatct ctctaaatgg tcaggatatt
    31201 aagaagattc ctcaaaaaga acttagagat aagattgggt ttgtaccaca aaaagctttt
    31261 ttatttagtg ggacaataga agaaaatata aggtatggaa aagaagatgc aagtattgaa
    31321 gaagtaaagc atgctgcaag tatagctcaa gctgatgaat ttatttctga tatggaagac
    31381 aagtatgact catttgtagc acaaggtgga aataatttgt caggtggtca aaaacagaga
    31441 atatccattg caagagcatt agttagaaag cctgaagtat atgtctttga tgatagtttt
    31501 tctgcactag attttaagac agataaaaga cttagaaaag cgttaaagaa tgaaatcaaa
    31561 gattcatcag ctataattat agcacagcgt ataagtacaa tcatggatgc taatcaaatt
    31621 atagttttga atgatggaaa aattgtcggt attggaaaac ataaagattt acttgaaaat
    31681 tgtgaagttt ataagcaaat tgcagattca caacttagta aagaagagct tgcataggat
    31741 agggaattaa tctgtgtatt tggaggtgtg taatatgagt gaaaaaagaa cagataaaca
    31801 agatatagag gtacttagtt ttgaagatgg tatgtcagca gaaaaagcta aggatgcaaa
    31861 aaaaactact aagagattat taaaatatat ggcaaaacaa aagtttaaaa tattaattat
    31921 ttttatttca gtattgataa gttcagcact tacagtttta gcacctatgg ttatgggaaa
    31981 agctattgac caactattta atggaataaa aactgcagtc caaacaggta caaaattcag
    32041 tgtgaatttt agtactatgg gaggtatagt atcaatactt ctaggattgt acttggttag
    32101 tgcaatattt atatatattc aacagtatat tatgtcagga gttgcacaaa atttagttct
    32161 tagcatgaga aaagatttaa gtgataaact caataaactt cctcttaagt actatgattc
    32221 tcataaaaag ggggaaacac ttagtatagt aaccaatgat ttagaaaaag tagcagatag
    32281 tttacaagaa ggattaatgc aacttattac agcagtagtt acagtagttg gttctattgt
    32341 aatgatgata agtataagtg taccacttac aatagtatca gctataacat tacttgtttc
    32401 attgggtatt actgttgtaa ttgcaagaaa atcacaagaa agattctctg aaaatcaaaa
    32461 ggcactagga gagttaaata gcaatataga agagatattt actggtcaaa tagtggttaa
    32521 agcatttagt aaagaaaagg atactataag aaactttaaa atattaaatc aaaatcttta
    32581 taatgcaagt agaagagcac agttttcgtc atatgcaata tctccaatta ttagatttat
    32641 aaacctaatt ggctatgtta ttatagcagt tgtaggtggt atatttgcat caactggagc
    32701 aatgactctt ggtagtatac aagcgtttat tcaatatgta aatcaagcat cagagcctac
    32761 tacagaaata tcttatattg taaatatgct tcaagcagca atagcatctg cagagagagt
    32821 ttttacagtt atggacgagg tagaagaaat aaaggatgaa gaatcttcaa aggttatcag
    32881 catgcctaga ggtaaagtac aatttgagca tgttaaattt ggatatagtg atgattttat
    32941 tcttatgaaa gatattaata tcaatttaaa tgcaggagat aagattgcaa ttgttgggcc
    33001 tacaggagct ggaaagacaa ctcttgtaaa tttacttatg agattttatg agatacaagg
    33061 tggaagaatt actattgatg gtgttaatat aaaagactta aagcgtggag aacttagaac
    33121 tatgtttggt atggttcttc aagatacatg gttgtttaat ggaagtataa aagaaaatat
    33181 tgcatatagt aagtcagatg caacaatgga tgaaattgta agagcagcaa aatcagcaag
    33241 agtagaccat tttataagga ctttaccaca agggtatgac actatattga ctgaggatgc
    33301 ttctaatcta tctcaaggtc aaaagcaact tctaacaatt gccagggcaa tactttcaga
    33361 cccatcagta ttgattcttg atgaagcaac atctagtgta gatactagaa cagaggtaga
    33421 aattcaaaag gcaatgaata acttgatgaa gggaagaact agttttgtta ttgcacatag
    33481 attatcaact attcgagatg ctgatttaat attagtaatg aaagaaggca ctattattga
    33541 gcaagggagt catgaggaac ttattgaaaa gaaaggattc tatgaagaac tttataacag
    33601 tcaattttca agtgaatatg atgaagatgt tgtatgatta tcaagtttta ggcaactaaa
    33661 tgattgataa atattaagtt aaatacagta acatataagg ttatatggaa taagagtatt
    33721 tctctatttc atataacctt ttttatttgt caaaataaga taaaatttcc tttctaaatt
    33781 agattgaaga atgtatttat caatataaaa gtaaaataac ccttaattag tgaagtaaat
    33841 attgcttgcc acatatatgc cataaagatg cacttatttt acggagattt aacaaattag
    33901 cataaagtat actctaaata tagaataaat caaatagagt taaataaatt gagaggtgat
    33961 ataaataatg gataaaaaat tgaactatag atttgaaatg aagcacaaaa taactgaagc
    34021 agatgtgcta gctttaaaat caagactcta tccaatcatg aagaaagata aaaatgcatt
    34081 aaaagatgga aagtatctta ttagaagttt atactttgat actcctgagg ataaagctct
    34141 tttggataaa ttgaatggcg ttgcattaag agaaaagttc aggataagat tttataataa
    34201 tgactgctcc tatataaggc tagagaaaaa aatcaaacat tacaatatga catcaaagct
    34261 atcagctagt ttaactaaga cagaagtgat aaagatattg aacaatgata ttgaatttct
    34321 aaaagaatcg acaaattgtt tattaagaga attttatttg aaaataaaat gtgaaagatt
    34381 agaggcaaag agtatagttg attatgatag agaagccttt gtttatccag ttggaaatgt
    34441 tagggttaca attgatagtg atattaaaac ttctgtaaat tcaatagatt tgtttaataa
    34501 acatttacca actgtttcga taattgatga gaacatgacg gtacttgagg ttaaatatga
    34561 tgaatttata ccagatttta taaaggattt aattcaaatt aataagacta cttctacagc
    34621 agtttcaaag tatgcttcaa gtagattata tatttaataa taaaagctat gatttatatg
    34681 aagctataca taaatgaggt taaaaaataa ataaaataaa aaataaatta aagattagga
    34741 aggatgattt taattatgac atttaacgat atttttaaat caagttttat agaaaacgta
    34801 tcaggttttt catttgtgga cagtgcttta gcactaggtt cagccttttt agtaggtttg
    34861 tttatatata tggtctataa aaagacttat atggggatta tgtattctcg accatttaat
    34921 gtgtcgcttg tagcacttac aatgcttaca acctttgtaa tattggcagt tacttcaaat
    34981 gtagttttat ctttaggtat ggttggtgct ttatctatag taagatttag aacagcaatt
    35041 aaagacccaa tggatttagt atttctattt tggtcattgg gttcaggtat agttttagga
    35101 gcaggtttaa ttccattagc tgtcatgggt tcaataatta tgggattgat tctaatattc
    35161 ttttcaaata aaacaatatc tgaaactcca tatatattga tggtaaactg taataatgaa
    35221 gattcagaag atatagcaac tgacaaaatt aaaaaagcat ttactaaata tcaaataaaa
    35281 tcaaaaagtg ttacaccaga aaaaggaata gaattagtgt ttgaaataag aatgaaagat
    35341 ggagagacaa gtcttataaa tgacttgagt caagtagatg gagttactaa tgccgttctt
    35401 gtaagttata atggtgacta tgtagcatag agaggagtaa tcttttatga aagataaaaa
    35461 atttaccctt cttatctcaa taatgattat atttttatgt gctgtagttg gaatttatag
    35521 tacatctggc aacaaaggtg ttgatttata cagtgatgta tatattgaaa aatattttaa
    35581 cagagacaag gttatggaag ttaatataga gatagctgaa agtgatttaa aagacatgaa
    35641 tgaaaatgct ataaaagaag aatttaaggt tgcaaaagta actatagatg gagatacata
    35701 tggaaatgta ggtataagaa ctaaaggaaa ttcaagtctt acgtctgtag taaatagtga
    35761 tagcaataga tatagttata agattaattt tgataagtac aatactagtc aaagtatgga
    35821 agggcttact caattaaatc ttaataactg ctattctgac ccatcttata tgagagagtt
    35881 tttaacatat agtatttgtg aggagatggg attgtccact ccagaatttg catatgctaa
    35941 agtatctata aatggcgaat atcatggttt gtatctggca gtggaaggat taaaagaatc
    36001 ttatcttgaa aataattttg gagatgtaac tggagaccta tataaatcag atgaaggaag
    36061 ttcgttacaa tataaaggag atgacccaga aaactactca aatctaattg ttgaaagtga
    36121 taaaaagaca gctgattggt ctaaaataac aaaactatta aaatctttgg atacaggtga
    36181 agatattgaa aaatatcttg atgtagattc tgtccttaaa aatatagcga taaatacagc
    36241 tttattaaac cttgatagtt atcaaggaag ttttgcccat aactattatt tatatgagca
    36301 agatggggta ttttccatgt taccatggga ttttaatatg tcatttggag gatttagtgg
    36361 ttttggtgga ggtagtcaat ctatagcaat tgacgagccg acgacaggta atttagaaga
    36421 cagacctctc atatcttcac tattaaaaaa tgagacatac aaaacaaaat accataaata
    36481 tctggaagag atagtaacta aatacttaga ttcagactat ctagagaata tgacaacaaa
    36541 attgtataac ctgatagcat catatgtaaa agaagaccca acagcatttt atacttatga
    36601 ggagtttgaa aaaaatataa cgtcttcaat tgaagattct agtgataata agggatttgg
    36661 taataaaggg tttgacagaa ataactctaa taataattct ggaaataaaa gtgatgaaaa
    36721 agaagttact gctgaattaa cattaagtga agtcaaaact aatatggata atgaaactga
    36781 gaataatact acaggtgata gtaaaaataa gaataatacg aatgatgata aaagtggaaa
    36841 tggtaataat caaaagccag aaggttctat gggtaaagga ggaaagtcaa taccaggagt
    36901 tttggaagtt gcaaaagata tgagtaaaac tataaaagct caattaaatg gagaaacttc
    36961 ttcaacaaag caaaattctg gtgatgaaag ctcaagtgaa aataaaggga ctgaaaagtt
    37021 tgatgaggat gtgggtggta tgccagaacc acctgaggga atggatggta aaatgccacc
    37081 aggaatggga aatatggata acggtgatat gaatggtaaa aatggcaata tgaatagaaa
    37141 tcaaggtaat ccaagagaag ctggaggttt tgaaaataaa ggagggggct ctatgagtaa
    37201 aacaagaaca tactcaaaat taattttagg tggagcttca atgataatta tgttgattat
    37261 gttagtaggt gtatcaaata taaatagaag aagatttata aagtcaaaat aagattgata
    37321 ttaaaaaata ctgttgagta gacatctact caacagtatt ttttagatat taagattaaa
    37381 tttatttaga aataagagtt agattttttt acttaa
//
LOCUS       DAMJVM010000030        37308 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.030, whole genome shotgun sequence.
ACCESSION   DAMJVM010000030 DAMJVM010000000
VERSION     DAMJVM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37308
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.030"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            205..915
                     /locus_tag="PC523_001600"
     CDS             205..915
                     /locus_tag="PC523_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2475133.1"
                     /translation="MIKIAICEDEKEQQELLKRYINQIFDALSVKCRLEVFNSGEELL
                     EGYSKDTDVLLLDIQMGQINGIDTARKVRVLDDKVEIIFITALIEYALEGYEVRAYRY
                     LIKPVKYNNLKDNIINCIKEVDIKNKYIMVKAQGSQTKLNVNEITYIEVQKETITIHT
                     LNEVYKINGTMSNIEKEIDCSRFFRCHKSFLVNLEHVKSIKQYVAILENSKEVPVSRY
                     RFKETKDKFFDLIEEKLC"
     gene            909..2246
                     /locus_tag="PC523_001601"
     CDS             909..2246
                     /locus_tag="PC523_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2475134.1"
                     /translation="MLDSVYSLAIINMVIFIALFFMLMNKIGYTYRCKTNIQLILILI
                     LSAILIINGMPFGIVPLHALIRIILILSLICKITYQINIKKATINSVIYSLLYILIGC
                     SMYWILQFSWKYVGHYILKANTVINFIIIFIGVYFFDILQKIYNNKKYYLYIILTIAV
                     NVVIVLFVNISTEKMDDLYTIVTKNHIEYSYNVYTMYFSLFMKNTFPYILFIVNVLLI
                     SIFINSIKSEKEKIKAEFVSEKLDMQYKYYFMVKESQEKMRQVYHDMNNHMKNIKSLK
                     NSSEDVNKYINSIEDEVKNTKNIYNTGNVLLDIILYEKSRLCMENNIDFNVGIDFSKC
                     EFIEMIDVSSIFSNLIDNAIEACSNIDDNNIEKYITIKSTFIKGYYIIRCENSKMNKV
                     IIKNNKIFTSKKDKFLHGIGIESIKSSIRKYNGELKIKDSEYKFIVTAHIPIE"
     gene            2423..4990
                     /locus_tag="PC523_001602"
     CDS             2423..4990
                     /locus_tag="PC523_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2475135.1"
                     /translation="MTTFKLAVSYMKRQKGKTVALLSCIVLAVMLIFSMIVIRDSGYD
                     SQIKEAKDLHGDYHIWFEGLEKDKTQDLTNEEGISKSNTSKELCEVVDKESGVKLYLN
                     SFDKGFLSSLGYKIEGREPIKDGEIVIEKEATRQMGISNPLNKNIDLMLLNKYIDDNE
                     INHMDSANKTFKVVGVIEKLDKYYDKSRYSITLRAFVYKDSNLPIKIKDTYNGTIYLK
                     SEKNIPQFITKMTKKIDTNVFNLHENGEVDLAKNQRQNSKFSKENIINSVLLVSVSTI
                     VIYNIFNIIFQDMTSQIGIMKSIGMSNKKVRNMLIIMSFIYIILGTLVGIVFGMIFSY
                     VGLRVVYGYSSMLTIQISSVICSFAVSIISVFISSFIVIKKFRKMSIIEAIRSSDKYE
                     KKSKNDNKKIDGKSKNLLISMATRNLWRNKPRTILTILAITLVGIMFILNLGAKSLLK
                     KNMEEGITGGSWSMSYGSVDKTVEGDSRSSESLFYKLDNNLIQKVNGMKGVRHVEPHF
                     YNHRGHILLSKNKLSKAYKDELDRKNSSYQEEHNNEYPLLIRGYSDDMLKQRQEFIEK
                     GENILSTTSGKYKKVILVNNTNSQVTHSFDAKIIDDVKIGDIIEIKLPVYRDGIEKYE
                     NFKVEVGAIMKELYASSQDGNTQAQGAQMIFKENDYKELTGQKEYNKLFVIVEKGQLY
                     SVERRLEETTKNYGSTEINGKGEELKLIGMQQSSEERLSVIYQFLTILILAVNIIFIM
                     RSNIITRRKELATLRAIGMSIKSIKKILIIESQMYGMVASIIGAIIATVYHNYGLAKT
                     NKVLLEGGYTRIIEPNIPFSQIIILFTIFIAMGFISIYVSKDKIEGTTITEGISQNN"
     gene            5049..5732
                     /locus_tag="PC523_001603"
     CDS             5049..5732
                     /locus_tag="PC523_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2475136.1"
                     /translation="MENRIMKKLAIKNIKKVYGKGDNKVYALNGIDLEIQPNKFTTIV
                     GQSGSGKSTLLHCMAGLDKPTDGKVFIDDLDLYTLNDDKLSKIRREEFGFIFQSYNLI
                     PVINVYDNIVLPISISKRKIDKDYIIDLIAKLGIKSQIDKFPNELSGGQQQRVAIARA
                     LANKPSIIFADEPTGNLDSKTTNEVVKLLKLCVYEYKQTLVMITHNAEIANNSDNIIN
                     ISDGKIVLI"
     gene            5995..6855
                     /locus_tag="PC523_001604"
     CDS             5995..6855
                     /locus_tag="PC523_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2475137.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKVLNIKSSDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMYALKLIGDSKEINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVEIDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            6845..8716
                     /locus_tag="PC523_001605"
     CDS             6845..8716
                     /locus_tag="PC523_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2475138.1"
                     /translation="MKYKDLNENIIKLTGGKENIKSVAHCVTRLRLTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSVKGERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILHSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWSMGIIIGGLKKILPKALHYFLVPVLSLIITLSVTLTIFGPIGMWIGDVLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPIALNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGL
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEMSNFTFMIIAVLVSFVGATVASYLLGIP
                     SDEKEEAKNANEKELELVVNEDNSRTISVGSVAKGQVISLEEVNDKAFSSGALGKGVG
                     VIPSESKSTVMSPVDGTITIVFQTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFDSDKIIEAGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            8736..10178
                     /locus_tag="PC523_001606"
     CDS             8736..10178
                     /locus_tag="PC523_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2475139.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYECLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     KKFDGKVRHWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNINIEVTDEDKKAFEEGRLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPVGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDNY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKFNGENLNLLDV"
     gene            complement(10457..10990)
                     /locus_tag="PC523_001607"
     CDS             complement(10457..10990)
                     /locus_tag="PC523_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDF2475140.1"
                     /translation="MNMKKEALEVLKNRRSIRKFKSNQVSKEELDLVLEAGTYAPTSK
                     GSQEPIIIAVQDEDTVSQLDALNARILNIEKTPHPYYGAPTILLILAPEDALIPVENC
                     SLVAGNLMNAAYAVGLGSCWIHRAKEMFESIEGKELLKKWGLPETLKGVASIALGYPD
                     CPHPKAASRKEGYILRV"
     gene            11128..11565
                     /locus_tag="PC523_001608"
     CDS             11128..11565
                     /locus_tag="PC523_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDF2475141.1"
                     /translation="MRMSTQFTVSVQMLLLIMVLDDRKVTSEMISKSTGGNPVMVRQL
                     FGKLKEAGILNVSAGRGATVLAKKPSEISLWDIYMAVEEYSSSELFKFHPKISEGCNI
                     GRFFKEILGNHLDEAIHAMADKMSKVSLAQLLEEWESVNKESK"
     gene            12753..13961
                     /locus_tag="PC523_001609"
     CDS             12753..13961
                     /locus_tag="PC523_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475142.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLI
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALIGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPNMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTSIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            13992..15371
                     /locus_tag="PC523_001610"
     CDS             13992..15371
                     /locus_tag="PC523_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2475143.1"
                     /translation="MIGSISINEIDKKRFKLDDLSRKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEIGVGGLATAIKASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGIKREMIENNLGGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYIRALSREAVESTYERLVKVAKG
                     ATMMTETEVEVEFLGGCYNTLNNHTLANLVSECMNEIKQEPWTQEELDFAAELDRQSP
                     QQYKDMISKYNLQEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYAAKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            complement(15748..17076)
                     /locus_tag="PC523_001611"
     CDS             complement(15748..17076)
                     /locus_tag="PC523_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475144.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDSFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQDAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTTCYIKESIENSINIKVCIGYGIGNDIKQAMSSAISANKESKINPLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(17686..18900)
                     /locus_tag="PC523_001612"
     CDS             complement(17686..18900)
                     /locus_tag="PC523_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDF2475145.1"
                     /translation="MRNCIIAQSGGPTAVINASAIGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYRINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFILETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASGTDLKQAADVGAYGIELSKKEYTGVMAII
                     NRTCNNPYKYEVGHTDISKIANKTKYFPKEWINEECNYITDEALDYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            19308..20024
                     /locus_tag="PC523_001613"
     CDS             19308..20024
                     /locus_tag="PC523_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HDF2475146.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTVGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIA
                     AGVKNCRKAIDEAATKFGLE"
     gene            20159..20785
                     /locus_tag="PC523_001614"
     CDS             20159..20785
                     /locus_tag="PC523_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2475147.1"
                     /translation="MKKEMIKDYLLITVSAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPFFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINSTCTQ"
     gene            complement(20969..22513)
                     /locus_tag="PC523_001615"
     CDS             complement(20969..22513)
                     /locus_tag="PC523_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2475148.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEKLNVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLQTLQVEK
                     SNLSDEYYINFLIHIAICIRRISIKRYINLSEGFFRELLEHEYFEKSKILCSNLGEAF
                     DVEFISEEVCYILLHVEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGNILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(22539..23180)
                     /locus_tag="PC523_001616"
     CDS             complement(22539..23180)
                     /locus_tag="PC523_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDF2475149.1"
                     /translation="MKYLIDSANLDEIQKCLKCGFKGITANPSMYLKENINFYEFIET
                     CTNLNPEILTAEVIGNNLYELINSCDKILNINKDTIIKINFSEIGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICDLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTVDGANTFERDWSKVNK"
     gene            23451..24602
                     /locus_tag="PC523_001617"
     CDS             23451..24602
                     /locus_tag="PC523_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2475150.1"
                     /translation="MINFYQPSRINFGQGTISELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLDLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNVDKFAKVARMLNQELEKVEDNEAAKTLGKEIER
                     FLKNVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            24615..25427
                     /locus_tag="PC523_001618"
     CDS             24615..25427
                     /locus_tag="PC523_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2475151.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAIKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGQEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVESLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIEFAGLGIAMGNATDEVKSIADFVTDTNVNDGVAKALRKVLS"
     gene            25453..25944
                     /locus_tag="PC523_001619"
     CDS             25453..25944
                     /locus_tag="PC523_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2475152.1"
                     /translation="MFNIFKKKENKVEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVSVFDTKHAITIKSNDNTNMLIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            26440..27141
                     /locus_tag="PC523_001620"
     CDS             26440..27141
                     /locus_tag="PC523_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2475153.1"
                     /translation="MINIAICEDNFVDRGILNDYLIRIMNENSYNISLFKSGEELLDN
                     YPDDLDILLLDILMKKINGIDTAKKIREFDSNVEIIFITSLLEYALQGYEVKAFRYIL
                     KPIEYEQLSINIIQCLDDINNKASKFVYIKCNSDIVKIDIESILYIEKISRYIFVYTE
                     DQVYKSKMNLKDIEKKLQHYKFYRCHLGYLVNVNKIDCLGSSEVSIYNHSIPVSRGKR
                     KILKEFIIDSKEKSL"
     gene            27522..28436
                     /locus_tag="PC523_001621"
     CDS             27522..28436
                     /locus_tag="PC523_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2475154.1"
                     /translation="MLLAYIFLIFLVFIIKFVVTKNNFIKNIHLIFFTFINIVSFLLI
                     FRGAAKDINVNFVDNIFIIIIGFTIVIFNLYLLSMMDKLLESRRIEIENKFMKEKIEI
                     EYKYIKKLQYSNENIRILKHDIKNHLICIRNIHNNKEAFDKYLDSVDIEVNKINDIYD
                     TGSIIVDAILDEKKIICDNYNIKLYVKANFTGCDFISAFDLCTIMSNLLDNAIESNLK
                     VKNSDCRNITVKANCEKYMLIIIVENNKSNKIIKSKQSIITDKKNKTIHGLGLKSVKK
                     SISKYNGDLFVEYNDENFKVKIIIPTNI"
     gene            28530..28703
                     /locus_tag="PC523_001622"
     CDS             28530..28703
                     /locus_tag="PC523_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475155.1"
                     /translation="MIKIKKITDKEKVVVSAQAKKPCSCAGQVLKYPPVSNSCLYDYD
                     DYNQPRWYEAQCQ"
     gene            28776..30035
                     /locus_tag="PC523_001623"
     CDS             28776..30035
                     /locus_tag="PC523_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HDF2475156.1"
                     /translation="MNYKFDDSVKLLKNGEYVILGNTKNGQWIKMTNYIYNIFKIGID
                     NSLDINELKKCLEDDEDRQFIQNLYENLLDMRIIYDEINNVAFQNKIASLEITNRCNL
                     KCVHCCVDADNLISEKEELSTIELKDAIKKIVEWNPMNIMLSGGEPMVRSDFFEILTY
                     LKSIYKGNIIVSTNATLINKNNVELLIKNVNKLEISLDGVNEQTCSIVRGKGVFKKVI
                     DSVNLIKSFGFDNITLSMAVGNKNEYLEEEFKDLCKNLGVGHMIRRFGPVGRGKENEH
                     LFLDNNKDEAYIPENFLKDNYDGNMGVTSCKAGIYEIFIRHNGDIYPCPSFMSDEYKI
                     TNLCDIQQISEIKDKYINKVDVFSILEKINPEIYKNCIDCNVNLFCWTCPGEIEELKD
                     SKASLFNKCSKIKNILYKRVWGNDGVC"
     gene            30029..31045
                     /locus_tag="PC523_001624"
     CDS             30029..31045
                     /locus_tag="PC523_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HDF2475157.1"
                     /translation="MLATFWITNECNMKCRYCYEGNNKNNKVMSKTLIDKSISYVLNQ
                     SNSLDDDELVIQIHGGEPFLEFNLIKYIISEFKSEKFNKNIIFSTTTNGMLLNNDILD
                     FIQLYIAELTISIDGAKNTHDKNRQSINGKGTHDIVIENSRKILKIKPNVRVRMTFDT
                     ETVGNLSNDIKYLISEGFRTIVPVPDMFETRWNNNHIEVLKNQIYQIKEIIGNSKDIL
                     VSLTDTNIYKYKGVCSGGKLSFNICPDGYIYPCTLAVGNKDFSIGNIYDGIDKTSRDK
                     FLSKSYLDNNDCKGCDLSNSCDGNRCKIINKLTRGSYFLPSPINCAIQNIKHEINISK
                     EIVL"
     gene            31035..32126
                     /locus_tag="PC523_001625"
     CDS             31035..32126
                     /locus_tag="PC523_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF2475158.1"
                     /translation="MYYSFVELRRNSLKRIKVALIDSGIDINHKYLKNNILYGKSYIY
                     KDNKIVVSNEIEDINGHGTSCASIIKSEYEYVDFIIIKIFDRDGLTNIKVLEESLNYL
                     ISIDVKIINLSLAIHGDINSCKDLYKICEKLKNQNKIIVSSLANYNYIEESYPSIFDN
                     VIGVKGNLLKKRSSFWYNSEYKYQCVVDDSDRIVADLGNSFKLVEKSNSFSAAKMSGK
                     IAKIIANKVDISFDGLSRILEELSECTNWKDEDLYTNKRRIDYKQYLYENNNINLNRV
                     LKVLVDNLNIGCNVDILFDSILFSKYIGLKNNNCIEILKKLENEFNIKIDYTKVYKED
                     LISIYTLTNLIEKESICEHFRNCKYLQKL"
     gene            32086..32751
                     /locus_tag="PC523_001626"
     CDS             32086..32751
                     /locus_tag="PC523_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2475159.1"
                     /translation="MNILETVNISKSYSIGDKKSYILKDINIKIKEGEFISIIGPSGS
                     GKSTLLHILGGLENPTTGIVKIIGKSIYDLSEIKLAEYRRRRFGFIFQQFNLIPVLNV
                     RENIEMPLMLDRAKIDNRFIKEIIEFLGLEEKENYYPNQLSGGQQQRVAIARALVSKP
                     DIIFADEPTGNLDTKNSDEVIKLLMKSIHKYNQTVLLITHDYKIAKNTDRTIKILDGK
                     LEL"
     gene            32752..34527
                     /locus_tag="PC523_001627"
     CDS             32752..34527
                     /locus_tag="PC523_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2475160.1"
                     /translation="MIQCQEGNKLKNTINMKKFLYLIIQGNKKLVIISFLMMIVVAMI
                     DLYIPQIIRNILDIAIKSKNITMLIKLILQFILINIILSNLELFLEYNYSKMKKKITT
                     KLKLKLLRHLSKLDGRFFSEIKTGNIMNIVEYDVMMLDRFGIELIFSLIVDIITAFIS
                     LYFLIKIQSKLLVIVVVIQIVISFTQYIFTKMISKYTQNIRSNAGSISNIVQEYISNI
                     MNVVIHKGELNFFTQFLSMQKNFVSKCIKLDMIMAGNVSLSKILNSLIVISIYGYGGY
                     KIINGEITFGELVAFQQYTGMLIGPCMNIIKSNAIVQQSLVSINRIFDLLNEEVNMKQ
                     ENTGEIFQSGITKKIRMDMIDVSFSYDKEDVLHNINMSLESGNIYGIVGGSGSGKSTL
                     IKLIYRLWDIESGSIRINDVDIRDYNLKNLRKNITIITQEPLLLNDTVFNNIILSNKN
                     INYELVVRICKELDIYDFIVSLPNEFNTIVGENGVKLSGGQKQRISIARAMLSNSSIL
                     VLDEATSALDNISQKDLLKSMKKFLNNKIVIIIAHRLSTIKDSDKIFVINNGEIIESG
                     SHKDLVSQNGYYCNLLEDKKEYSYC"
     gene            34697..37228
                     /locus_tag="PC523_001628"
     CDS             34697..37228
                     /locus_tag="PC523_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2475161.1"
                     /translation="MRLSLAYVKKYFGRSISIALSIIISISLIITVGILTKNTQKASV
                     DRIKYESGDYHVKVKNISGNVISDYKKQFDISMMGVSSYYDSSEFNGKLMLNLIKSNE
                     DYIKIYNSKILNGRFPTSDNEVALEEWVMLNLGLKLDINQNIKIKLYNSKETKNYKVV
                     GILKDRPYYKSIGMAELILPFTENIIKETNLEAQIRFKKDIYRNLSKLMNDNHISRKN
                     VSENRILLDALGESNGLNYDFIFTCIISMIVSGVVIYSIFRISIIQRISEYGIIRALG
                     ANIFNVFNIIFVELFAISLVSIPIGVLIGIISSKYITLFMGNLLIGNIVKINNVEINL
                     DVVMISIITVLVNILIISFLTCTNVNKISPIKCIKKDISKKNSRESSILNTRRLLNLL
                     PIEYIISLKNILKNKKNFVIIVLSMSGGCIIFILTNFTNILDERQLEYTSKVATENSD
                     YNISFAPVTPLNIGISEENLKQIRQLEGIENVKTSQVMYSRMILNKDNINNMNYFEKV
                     NKSAYNKKALNGLLVKKDNEYILKSNLYGYDNSSIDAQKSFVISGDIDLDKMNNGNVA
                     ILYMPDPSLFTNIKDSRPVLNLKVGDIVKLRFRSDYVSDENFAKLKDKGEYVEEEFKI
                     GAIVSNILNVDGYYAGMDSASIIISQNQFKNIVGIHSYRMLDIKKIPTVDNIELGKNI
                     IDISSKIQGSIVRDFTQEIKNIKIIEDNKKILIYTIILVLFTISFINIINNINYNLYS
                     RINEFGVIRAIGLDNNSFRKMLLFEGVVYGLISASVSITFGILGQSILYNILKGNLMN
                     AKFIIDYKSYILVIILTIILGVVATYLPSKKVKKLSIIESINIIE"
BASE COUNT        14771 a         3814 c         6031 g        12692 t
ORIGIN      
        1 tatgaaaaat atatacataa tattatctta aatattaaaa gagtatactg cataaaaaat
       61 aataatcact tatgagtatt ccataataac tcataaatga ttattattta agaataaagt
      121 acgtaactga ataagaagat taagaaaagt tttagctttg ttaagaataa tttaagtata
      181 taaatgtgta tggggtggaa ttagttgata aaaatagcaa tatgtgaaga tgaaaaagaa
      241 caacaagagt tgttgaaaag atatataaat caaatatttg atgcattgtc tgttaaatgt
      301 agattggaag tatttaattc aggggaagaa ttacttgaag gatattcaaa agatactgat
      361 gtacttttat tagatataca aatgggacaa attaatggaa tcgatactgc gagaaaagtt
      421 agggtactgg atgacaaagt agaaattata tttattacag cattaataga atatgcacta
      481 gaaggttatg aggtaagggc ttatagatac ttaataaaac cagtaaagta taataattta
      541 aaagataata taataaattg tataaaagaa gtggatataa aaaataaata tattatggta
      601 aaagcacaag ggagtcaaac taaattaaat gtaaatgaaa taacatatat agaagtgcaa
      661 aaagaaacta taactataca cactttaaat gaagtttata aaataaatgg cacaatgagt
      721 aatattgaaa aggaaataga ttgtagtaga ttttttagat gtcataagag ttttttggtt
      781 aatttagagc atgtgaaaag tataaaacag tatgttgcta tacttgaaaa cagcaaggaa
      841 gtacctgtta gtaggtatag gtttaaggag acaaaagata aattttttga tttaatagag
      901 gagaaattat gttagatagt gtgtatagct tagctattat aaatatggta atatttatag
      961 ctttattttt tatgttgatg aataaaattg gttatactta cagatgcaaa actaatattc
     1021 aacttatatt aattttaata ttatctgcaa tcttaattat aaatggtatg ccttttggga
     1081 ttgtgccatt acatgcttta ataagaataa ttttaatatt atctttaatt tgtaaaatta
     1141 cttatcagat taacataaaa aaagctacta ttaattctgt tatatatagc ttgttatata
     1201 ttttgatagg gtgtagtatg tattggattt tacagttctc atggaagtat gtaggtcatt
     1261 acatacttaa agcaaatact gtaataaatt ttattattat ttttataggt gtttattttt
     1321 ttgatatact acagaaaatt tataataata agaagtatta tttatatata attttaacaa
     1381 tagcagttaa tgtagttata gttttatttg taaatatatc aactgaaaaa atggatgatt
     1441 tatatacaat agtaacaaag aatcatattg aatatagtta taatgtatat acaatgtatt
     1501 tttcactttt tatgaaaaat acatttccat atattttatt tatagtaaat gtattattga
     1561 tatctatatt tataaattct ataaaatcag aaaaagaaaa aattaaagca gaatttgtaa
     1621 gtgaaaagtt agatatgcag tataaatact attttatggt aaaagaatca caagaaaaaa
     1681 tgagacaagt ataccatgat atgaacaatc atatgaaaaa tataaaatcg ctaaaaaaca
     1741 gtagtgagga cgtaaacaaa tatattaata gtatagaaga cgaagtaaaa aatactaaaa
     1801 atatttataa tacaggaaat gtactattag atattatctt gtatgaaaaa agtagattat
     1861 gcatggagaa taatatagat tttaatgtag gcatagattt tagtaaatgt gaatttatag
     1921 aaatgattga tgtaagtagt atattttcaa atttaataga taatgcaata gaagcatgta
     1981 gtaatattga tgacaataat atagaaaaat atataactat aaaaagtacc tttataaaag
     2041 ggtattatat aatcagatgt gaaaacagta aaatgaataa agtaataatt aaaaataata
     2101 agatttttac ttctaaaaaa gataaattcc ttcatggcat aggtattgag agtataaaat
     2161 cttcaataag aaaatacaac ggagaattaa aaattaagga tagtgagtat aaatttatag
     2221 ttactgccca tataccaatt gaataaaaat tcaatagatt gatgcattta aaaagcttat
     2281 aagctagtct ttaatatgct agttgacggt gaaaaaatac cacttgtcgg acttatattg
     2341 aataaaaatt ttaaaaatgt tacctttaca aaaaaggtag cattttttat acaaggattt
     2401 aatagaagaa ggaggtaaag ttttgactac atttaaacta gcagtttctt atatgaaaag
     2461 acaaaaagga aaaacagtag cactactatc gtgtatagtt cttgctgtaa tgcttatttt
     2521 ttctatgatt gtgattaggg attctggata tgattcccaa ataaaagagg caaaggattt
     2581 acatggtgat tatcatatat ggtttgaggg actcgaaaag gataaaacac aagacctcac
     2641 aaatgaagaa ggtatatcaa agtcaaacac ttcaaaagag ctatgtgaag ttgtagataa
     2701 agaaagtggt gttaagcttt atttaaattc atttgacaag ggttttctaa gttctttggg
     2761 atataaaata gagggaagag aacctataaa agatggagaa atagtaatag aaaaagaagc
     2821 tacaaggcaa atggggatat ctaacccatt aaacaaaaat atagatttga tgcttttaaa
     2881 taagtatatt gatgacaatg agattaacca tatggatagt gcaaataaaa cattcaaagt
     2941 tgttggtgtg atagaaaaat tagataaata ttacgataaa tctaggtata gtattacact
     3001 tcgagctttt gtgtataaag actccaattt gccaataaaa ataaaagata cgtataatgg
     3061 aacaatttat cttaaatcag agaaaaatat acctcaattt ataactaaga tgacaaaaaa
     3121 gatagatacc aatgtcttta atctgcatga aaatggtgag gttgacttag caaagaatca
     3181 aagacaaaat tcaaaattca gcaaagaaaa tattataaat tcagtgcttt tagtcagtgt
     3241 atcaactatt gtaatataca atatttttaa tattattttt caggatatga ctagtcaaat
     3301 aggtattatg aaatctattg gtatgtcaaa taagaaagta agaaatatgc ttattattat
     3361 gagctttatc tatataattt taggaactct agtaggaata gtatttggaa tgattttttc
     3421 atatgttggt ttaagagtag tgtatggata cagctctatg ctaacaatac aaatatctag
     3481 tgtaatatgt tcttttgcag tttcaatcat atcagtattt atttctagct ttatagtaat
     3541 taaaaaattc aggaaaatgt ctataattga ggctataagg tcaagtgata agtatgaaaa
     3601 aaaatcaaaa aatgacaata aaaagataga tggaaaaagt aaaaatttac ttataagtat
     3661 ggcaactaga aatctatgga gaaataaacc tagaacaata ttaacaatat tagcaattac
     3721 attggttgga ataatgttta tcttaaatct aggtgctaaa agtttgctaa aaaagaatat
     3781 ggaagagggg ataacaggtg gttcatggtc aatgtcatat ggaagtgttg ataaaactgt
     3841 tgagggagat tctagaagtt cagaatcttt attttataaa ttagacaata atttaataca
     3901 aaaagtgaac ggcatgaaag gtgtaaggca tgtagaacca catttttata atcatcgtgg
     3961 ccatatttta ctttctaaaa ataagttatc aaaagcttat aaagatgaat tagacagaaa
     4021 aaattcctct tatcaagagg aacataacaa tgaatatcca cttttgataa gagggtatag
     4081 tgatgatatg ttaaagcaaa gacaagaatt tattgaaaaa ggggaaaata tactaagtac
     4141 tacatctggc aagtacaaga aagtcatctt agttaacaat acgaattcac aagttacaca
     4201 ttcttttgat gcaaagataa ttgatgatgt gaaaatagga gatattatag agataaagct
     4261 acctgtatat agagatggta ttgaaaaata tgaaaacttt aaggtagagg ttggtgctat
     4321 catgaaagaa ttatatgcat caagtcagga tgggaataca caagctcaag gggctcaaat
     4381 gatatttaaa gaaaatgact ataaagaatt aacaggtcaa aaagagtata ataagctttt
     4441 tgtcatagtt gaaaagggac aattatattc tgtagaaaga agattagaag aaaccactaa
     4501 gaattatgga tctactgaaa tcaatggaaa aggagaagaa ctaaaactta ttggaatgca
     4561 acaaagttca gaagaaagac tttctgtaat atatcagttt ttgactatat taatacttgc
     4621 ggtgaatatt atttttatta tgagaagtaa tattattaca agaagaaaag agctggcaac
     4681 cttaagggca attggaatga gtataaaaag tattaaaaag atactaatta ttgaaagtca
     4741 aatgtatgga atggtagctt ctataatagg agctataatt gcaacagtat atcataatta
     4801 tggtttggct aaaacgaaca aagttctttt ggaaggtgga tatactagga taatagaacc
     4861 caatattcct ttttctcaaa taattatact atttacaatt tttattgcta tgggatttat
     4921 ttctatatat gtatcaaaag ataagataga aggtacaaca ataacagaag gaatttcaca
     4981 aaataattaa ggctatggaa tgacaagata aaaatgtaat actatttagt gggatttaaa
     5041 taagaagaat ggagaaccgt attatgaaaa aattagctat aaagaatata aagaaagtat
     5101 atggaaaagg ggataataaa gtatatgcat taaatgggat agatttagaa atacaaccta
     5161 ataaatttac aactatagtt gggcaaagtg gctcaggcaa aagtacatta cttcactgta
     5221 tggcaggtct tgataaacct acagatggaa aagtatttat agatgattta gatttatata
     5281 ccctaaatga tgataagtta tcaaagataa ggagagaaga gtttggattt atatttcaaa
     5341 gttacaatct aattccagtt ataaatgttt atgataatat agtacttccc atttcaatta
     5401 gtaaaagaaa aatagataaa gactatataa tagatttaat agctaaacta ggtataaaat
     5461 ctcaaattga taaatttcct aatgaattat ctggaggtca acaacaaagg gtagcaattg
     5521 ctagagcact tgcaaataaa ccttcaataa tatttgcgga tgagcctaca ggaaacttag
     5581 atagtaagac aactaatgaa gttgtgaaac ttctaaaact ctgtgtatat gagtataaac
     5641 agacattagt tatgataact cacaatgctg aaatagctaa taattcagat aatattataa
     5701 atattagtga tggtaaaata gtattaattt agtatataaa ctttgcaaga taatcgccta
     5761 cagagtctgt agttatataa ttaaaaaaat atttaggaaa ccgttgacag atttacatag
     5821 atagtataac atataggata taaaattaaa cgaaaaagaa ttttaattat ggcgacgtag
     5881 tcataatttt gagtatgtta ctgattagca ggcagaactc aagtatatat gtgaaaaata
     5941 gagggatact ctatacatat atgcttgagt tctttttatt gaagggagga atatatgaaa
     6001 ataattcaga ttttaaataa taatgtggcc ttggtaaaaa gaggcaaaaa tgaaattatt
     6061 gtatttgtta aaggaattgg ctttaaaaaa agagtaggac aagtaataaa tgaaaatgaa
     6121 atagagagaa catatatatt agattcatac gatatgttag aacactttag ttatctatta
     6181 caaaactcag actccaataa tatagttctc attaatgaaa taattgaata tggtgagaaa
     6241 gttttaaata taaagtcaag tgattatcta agtttaacct tactagacca tttagagttt
     6301 ttacttaaaa gatgtgaaaa gaatcaattt ataaaaagtc ctttggtatg gaatgttaaa
     6361 agattttatc ccaaacattt tgaaattgga atgtatgcac tgaaattaat tggtgatagc
     6421 aaggaaatta atcttcctga tgatgaggca gtatcaatag cacttcattt tgtaaatatg
     6481 gaggtaaaca aagagtctca tgatagtacg attgtagagt taagaacttt agcagatata
     6541 gtttctataa taaaatatca ttttaatgta gagatagatg aaacttcaac aaattatatg
     6601 agatttacta ctcatctaca gtactttatc caaagaatta tgtcagatga agcttcaata
     6661 gatgaagata attctataga tttatataaa caagtaagtc aactttaccc aaagtcattt
     6721 ggaagtgttc aaaagataag aaaatatata aaatcacaat ttaatgtaga tatatctata
     6781 agtgaagaaa cttatttaat gttgcatata aatagggtga ctcaaagact ggaggcaaaa
     6841 tcagatgaaa tataaagatt taaatgaaaa tataataaaa cttacagggg gaaaagaaaa
     6901 tataaagtcg gtagcacatt gtgttactcg tttaagactt actttaaatg ataggagtat
     6961 cgctcaaaca gaagaaatta aaaaattaaa aggagtaatt gatgtagttt ctaatgatgt
     7021 tgcttatcaa attataatag gtactcatgt tgtagatgta tataacgagt ttatgtctat
     7081 gataggttta agttcaagtg aatcaagtgt aaaaggtgaa agaaaaaagg gaattagagg
     7141 tatcaaaggt atatttaatt ccatctttgt ggtagtctca gaaactatga cttcagtaat
     7201 tgaagtacta cttgcagctg gtatacttgc aagtatatta gcgcttttaa atatgtctgg
     7261 tatacttcat agtgatagtc ctacatatca aatattagat acattaagaa gcgctacatt
     7321 tcacttctta ccagtattaa ttgcaatagc atcagcaaaa agacttaatg taaatcccta
     7381 tatagcagta gtgttagcag ttacattact atcttcatca atagatggtg tagaaggatt
     7441 aagtttattt ggtataaatt tacaggctac aacctatgca aacagcttta ttccaatact
     7501 tttaggagta tggtcaatgg gaattataat tggaggtttg aaaaaaatac tacctaaagc
     7561 actacactat tttttagttc cagtattatc tttaataata actttatcag ttacattaac
     7621 tatatttgga cctattggta tgtggattgg tgatgtatta aactatgcat gtatgttttt
     7681 aattgataca ttaggaaatt ggagtgttgt tgcattatat gcagctttac aaccactctt
     7741 aatagtcttt ggcgcagcaa attttactat gccaatagct ttaaattctg tgacaacatt
     7801 agggtatgac ccaattttta tgggagcagc aacaatatct gatattgcag tttgtggtgc
     7861 aatgtttgga tatttcttaa aaactaggaa taaagagcaa aagcaattat ttgggacagt
     7921 gagttttagt gcacttatgg gagtcacaga accttcagtg tttggtgtgt ttatgaaata
     7981 tagaagacca tttgtagcag taatgatagg cggaggctta ggtggcctaa tagcaggatt
     8041 agcacaagtt aaaacatatg gaatggtttg gggattagct gcattaccaa catatcttgt
     8101 tggtggagag atgtctaact ttacatttat gataatagct gtattagtat catttgttgg
     8161 agcaacagtg gcttcttatt tactaggaat accttcagat gaaaaagaag aggctaagaa
     8221 tgctaatgaa aaagaattag aattagtagt taacgaagat aattcaagga ctattagtgt
     8281 tggaagtgtt gcaaaaggtc aggtaatttc acttgaggaa gttaatgata aggcattttc
     8341 atctggggca ttaggtaaag gggttggagt tatacctagt gaatcaaaat caactgttat
     8401 gtctcctgta gatggaacta taactatagt atttcagacc aaacatgcct atggtatagt
     8461 gactgatgaa ggattagaaa tattaattca tattgggata gatactgtaa acttggatgg
     8521 aaagttcttt gaaagtcttg taacacaaaa tcaaagggtt aaaaaaggag atgctttagc
     8581 agtatttgac tctgataaaa ttatagaggc aggatttgat tcaactataa taactgtagt
     8641 tacaaatact agcgattatt tggatgtaat ctcatcaaat gatgaatcag aagaactatt
     8701 aactgtaata gtataaattt taggagggga aatttatgaa tattaaagat acatttttct
     8761 ggggaggaag tattgcagcc catcaatgtg aaggttcatg ggatagtgat aacaaaggac
     8821 ctgcaattat ggattttgta actaaaggtt cttatgaaac accgagagta attactgata
     8881 aaatagaaga aaaattggat tatccatctc ataatggaat agatttttat aatagatata
     8941 aagaagatat agctttattt aaagaaatgg gatttagtgc attaagaata tctatagatt
     9001 ggtcaagaat ttttccaaat ggggatgatg aaaaccctaa tgaattaggg attaaatact
     9061 atgaatgtct gatagatacc ttaatagaaa ataatataga acctattgta actctatatc
     9121 attttgaatt acctatgaac ttagtacata aatatggttc atggaataac agaaaactta
     9181 tagatttgta tttaaagtac agtgagactg taataaaaaa gtttgatggc aaggtaaggc
     9241 actgggttac atttaatgag atgaaccata ttgaccctca aacagagcac tcggatattt
     9301 ttacttatat gatagcaggt attaagtata gtgaaataga aaataaggtt caaacactgg
     9361 caaacgtagg gtataatatg actttggcag gtgtaaaggt tgcaaagttg gctagagaaa
     9421 tcaatccaaa caataaagtt ggatgtgtat ttggactaaa tccaatttat ccatacaatt
     9481 gtaatcctag taatgtgtta aatgcatttt tagataatga ccgtgattat tatcaaattg
     9541 atgcaatgtg caatgggaag tttcctcaat ataaattaaa agaatatgaa aaaaataata
     9601 taaatattga agtgactgat gaggacaaaa aagcatttga agaaggtaga ttagatttta
     9661 taggtcttaa ttattatttt tcaagtgtat ctactccaaa agaaaattta tcaggtgata
     9721 aatctttatt tggtggtatt caaaatccat atttggaaca gagtaagtgg ggatgggcaa
     9781 tagaccctgt aggtttaaga tttactttga actatattta cagaaagtat caattaccta
     9841 tcttaattac agaaaatggt ttaggggcat atgataaaat tgaagaagat ggaactataa
     9901 atgacaatta tagaattgag tatttaagta agcatctatc tcaaatgaag tctgcaattg
     9961 aagaggacaa tgttgaatgt tttggatatt tgatgtgggg accaattgat ttggtcagtg
    10021 caactacagg tcagatgagt aaaagatatg gatttatata tgttgactta gatgatgaag
    10081 gcaaaggaag ttttaagaga tataagaaaa aatcatttga ttggtacaaa gaggtaataa
    10141 aatttaatgg agagaatcta aatttgctag atgtataaaa tgaaatatgt gttatatgta
    10201 ttatgattat accttcgatt attataaact acaaataaat ttaatttacc atttttcggt
    10261 aagtcactaa aataataaag ttaaacaagc atttataagt ttagttaatg tttaaagtaa
    10321 aagcactctt ttaaagtatt atctatatat cagtaatgtt ttaaatgagt gcttatgtaa
    10381 taattttggg ttaaatatag aataaaaaat taaaagcagt ttacttaaaa ataaagtaat
    10441 tttaattttt atgtatttat actctaagaa tatatccttc cttacgagaa gcagccttag
    10501 ggtgtggaca atcaggatat ccaagagcta tagatgctac accttttaaa gtttcaggta
    10561 agccccattt ttttaataat tcttttcctt ctattgattc aaacatttct tttgcacgat
    10621 gtatccagca agaacctaaa ccaactgcat atgcagcatt cattaaattg ccagcaacta
    10681 aactacagtt ttcaactgga ataagtgcat cttctggagc aagaataagt aaaatagtag
    10741 gtgcaccata ataaggatga ggtgtttttt ctatattaag tatcctagca ttaagtgcgt
    10801 ctaactgaga aactgtatct tcgtcttgta ccgctattat tattggttct tgtgagcctt
    10861 tgctagtagg tgcatatgta ccagcttcaa gaactaaatc aagctcttct ttacttactt
    10921 gatttgattt gaattttctt atacttctac gattttttaa aacttctaaa gcttcttttt
    10981 tcatattcat acactcctta tgttatagtt taattgtagt cttattagct acaattaaac
    11041 tataacataa aataattgta gcgtcaatag ctacaattga caatttattt tataaattat
    11101 ataatactga aaagggaggg atgccaaatg cgtatgagca ctcagtttac agtatcagtt
    11161 caaatgctat tattaataat ggttttagat gatagaaaag taactagtga aatgatttct
    11221 aaaagtactg gaggaaatcc tgtaatggta cgtcaattgt ttggcaaact aaaggaagca
    11281 ggaatcttaa atgtatctgc aggtagagga gcaacagttc tagcgaagaa gccttcagag
    11341 atttcattat gggatattta tatggcagtt gaagagtata gttcaagtga attatttaaa
    11401 tttcatccta aaatatcaga gggatgtaat ataggacgtt tctttaaaga gatattaggt
    11461 aatcatttag atgaagcgat acatgcaatg gcagataaga tgtctaaggt gtctttagct
    11521 cagttgctag aggaatggga aagtgtaaat aaggagagta agtaataatt gaacttattt
    11581 attgttgata ttattgaaat attaatatta ttaaacttga aggtcatatt gagagattat
    11641 caatatggcc ttatttatac atttagtaat ggcatctata taattttact tataatctat
    11701 atcaaacaag ttaatatata taagtatttt atctatgact tttattcttt taataatata
    11761 tatataaatt tctagcaaga aatagtttac atttcactac tgtggtgtgt atactactat
    11821 gaatatttaa acttttccta tagaacttta ggcatattaa tatattaatg attaatatgt
    11881 ttgattttta aataatacta aatctaataa agaattatgt tagatggaaa aataaaaaaa
    11941 ctataaatta tataatagat tagtggttat agagtaaaaa taagatggca gtaaacttta
    12001 aggtatatta ataagaagcc acatctttaa ttttagcata agattatgaa atcataaatt
    12061 tacaaattat tgtcagaata cattgacaaa tttatggttt gaaattataa tctatgttaa
    12121 gaattgtttg acaatgtaaa actagtgatt ttagaattta gaagaattat tttttatgtc
    12181 taatttagga ataatttaag actattatag gtttataatt tatcattaat ttaattagat
    12241 aaataggttt tgtctctagg aataagattt ctaaaaacac tagaaagagt ataatttata
    12301 aaggggggag tagaagagaa aaaagtatat ctacagaaag acttaaaaca agacatattt
    12361 taataatgtt aatgaatctg tttttaagat ttgttaagga ttttaagaat aatttaagac
    12421 tcttataaaa tgggattctt atagttagaa tttagataag tataagtctt ctaagaagta
    12481 tataaaaatt aaaaatagtt atagcgtaat tctaaatttg taaaaagatg tataagatta
    12541 aaaagaaatt aagtatatga ataactatgg ttaagattta agaaattaac cgtggttata
    12601 tttattcaga tattttaaga ataatttaag acgcttaaaa atcatcagaa aatatcaaac
    12661 ttaccatatg tagccccaaa tagctataga gtgtacattt tatgaatatt ctttcattta
    12721 taaaaaatta ttttatttag gaggaaacaa caatggaaaa tttttggaat ccgcttactt
    12781 gctttactgt atgtgtaatg atttatgctt ttagtgagtt tttatctaag aaaacaaaag
    12841 gtgcaatttc aacattgcta tttgtatgta ttatattttt aataggattt tggacaaata
    12901 ttttacccaa agacattaca ggagcatcag ggatattagc tgcaatgagc aactttggta
    12961 tagctttatt aataactaac ttaggaacac ttataaatct tgaagactta tgtcatgaat
    13021 ggaaaacggt tgtaattgca ttaataggta ttgtaggaat aggggttggc tgtttaacag
    13081 taggtagtat gttatttggt agagagtatg ctttaatagc agctcctcca atagcaggtt
    13141 ctactgttgc tggtattatc gtaacaactg ttgcagaagc tgcaaataga cctgaacttg
    13201 ctgcatttgt aattctagta ttatcatttc aaaagttctt tggcattccg ataactacat
    13261 attgtataaa taaagaatta aaatttaaaa gagataatgg ggattttttg aaagatgagt
    13321 caaaaagtaa ttttaaactt ccaaacatga gaatttttaa agaagattta ggtaaaaaat
    13381 catctacttt agtgtatctt gcaaagcttt catttgttgc tatgattggt aattttgtag
    13441 gattggctac tttaatacca ggatttacac ctgcaaacta ttatttaaat cccaacatag
    13501 catgtttgtt agtggggttg atttttacac gaataggttt tttagaaaaa ggaattttag
    13561 ataaatctca atctaatggg attataatgt ttggttgtat gttaatgtta cctggaggac
    13621 ttgctaaggt aactccaaca gcattgtttg ggatgattgt tccaactata ggaatacttg
    13681 ttgtaggctc cgtatttata atattattta cttcaattgt aggcaaacta ttgggatatt
    13741 ctccaagaat gtcagctgca attggtgtaa cttgtatgct tgcttatcca gcaacacaga
    13801 taatttctaa tgaaggtatt gatgcaatgg aaggaactga agaagaaaaa caaagagcaa
    13861 cagattattt gttaccaaag atgattatag gaggatttgt tactgtaacc attgcatcag
    13921 tagcatttgc tagtataata ggtccaatta tatttggttg atagtaaata gaattatagg
    13981 aggaatagga aatgatagga agtatatcta taaatgagat agataaaaaa agattcaaac
    14041 ttgatgattt gagtaggaaa atatgggaaa atccagaaaa agcatttaaa gaatttaaag
    14101 catgtgaaaa tacagcaaat tttttaagaa gtgaaggatt tgatgtagaa ataggtgtag
    14161 gaggtttagc cacggctatt aaagctagtt ttggttcagg caaaccagtt ataggtttta
    14221 tgggagaatt tgatgcgtta cctggtttaa atcaaaaagt ttcaacaaag caagaagcat
    14281 ttgaattggg tgcttatggt catggatgtg gacataattt attatgtaca gcacatgtag
    14341 gtgcagtagt agggattaaa agagaaatga tagaaaataa cttaggtggg actatagtat
    14401 tttatgcatg tccaggagaa gaacaattaa caggtaaagg atttatggct agaggtggag
    14461 cttttgaagg tttagactta gctataaatt ttcatccaaa taaaattagt gaagctacaa
    14521 ttggtacttc aacagctgtt aattctgtaa aattccattt taagggaaaa actgcacatg
    14581 caggctcaga ccctcaaaat ggaagaagtg cactagatgc tgtggaactt actaatgtag
    14641 gagctaacta tttgagagaa catgttactt ctgatgtaag aatccattat acaattacag
    14701 atggaggagt agcaccaaac atagttcctg ataaagcatc agtatggtat tacataaggg
    14761 ctttaagtag agaagctgta gagagtacat atgaaagact tgtaaaggta gcaaaaggag
    14821 ctacaatgat gactgaaact gaagttgaag tagagttttt aggaggatgt tataatactt
    14881 taaataatca tactttagca aatcttgtat cagaatgtat gaatgagatt aaacaagagc
    14941 cttggactca agaggaattg gattttgcag ctgaattaga tagacaaagc ccacaacaat
    15001 ataaggatat gataagtaaa tataatttgc aagaaggaac tcatctatat tatggtggag
    15061 gaaatgttac aaactttaat agttatggct ctactgatat tggagatgta atgcatatag
    15121 ttccaacagc atactttttt acagggtgta cgaatctagg tgcgccaggt catagctggc
    15181 agtttacatc ttgtgcagga agttctatag gagaaaaagg tatgatatat gcagcaaaaa
    15241 ttatggctat gtttggagct aaaatattaa acaatccaga gatagctaaa aaggcaaaag
    15301 aagaatttga taaatctatg aatggtaaaa catataaatg tcctattcca gatgatattc
    15361 caacgcctta aaaagatatg aagtttaata tataaaatta taatttttat gtcaacatca
    15421 tttcatacta aaagaaagtg agattttact aagagtttag gagtattttt atggattgga
    15481 atgggaagta ttagtcaggg aaataatttt ggtatgcata atcctaaggc aatttttaat
    15541 gaatagggat tagtttacgg ttcagcttta tttgctaatt atgcagtaaa atggttagaa
    15601 aataatttta gtagagaata acataaaata ttattaaata tcataaaagg tatatcttaa
    15661 aaacaaatgt caagatatac cttttagaga attatatata attttactta atatcagatg
    15721 ataaaatatt tttatgtatt ttggatttta atatttattt tctgatataa ataatttgta
    15781 taccctttca ggtctacctt ttgttgagtt ttgtttttca tataatatag tagcgtttcc
    15841 agactttaag agactactta gaaatctatt agcagttctc actgttacat ttaaacatga
    15901 agacaattct ttaggagtaa cttcatctct ttgtagaatt tcagtaacag atataatctt
    15961 ttgaatagta agagtagaca atttagaact tttagcaatt gtatttatat gtggagttac
    16021 tttatttgaa acagtaagta acttatcaca taataaaggt cctattgtct cataattttc
    16081 gttaactaag aaactgtagt ttaaagggtt tattttagat tccttattag cacttatggc
    16141 actagacata gcttgtttta tatcattacc tattccatat ccaatacata ccttaatatt
    16201 tatactattt tcaatggatt cttttatata acacgtagta aaatttttag ttaatgattt
    16261 aatcgtttca catttagtga aaattttaaa agaattacta tttttttcaa ttataaaatc
    16321 ggttaactta tcttttttta tactatttaa tattttataa acttggtcta aagtagaatc
    16381 atcataattt gtatctttaa ctgttatata tattacactt ggttgatttc ccttcattct
    16441 atgtaaatga atgtctttta ttaagttatt aaaagtattt tttaataaat ctatatcagg
    16501 atatatgaaa tgaaagttta ctcctgcatc ttgtaacata ggaacaatac tactaaaacg
    16561 agtagtagaa aaatcaattt tcccttcatt ccatagatta atatgttttt ttgcaatctt
    16621 tttttcgagt tttgctatat cagataaact cattttagat gcattttcat caatactatc
    16681 cattaagtct ccaaatgtac catttgataa ataatatgat atatcttttg gattatctgc
    16741 ccagtcagtt aagtcaaagt atgctctaga aaaatctaat gtattatatt taaagataag
    16801 ctttagaaac atatcatagt aactttgcaa atcagtacca aaatcaacta gaggtttttt
    16861 aatagttcca gccttttttg taatagcttt tttggggata ggtccactaa ctgcaaatcc
    16921 atcaaaacta tcttctattt ctaaatacaa atctccaaca tgagaaaaat ctctatagat
    16981 atatatttca atttctgcat taatatttaa tttttttata gaattatcta caaactcttt
    17041 taaaaattcg gttgttatga ctgctatttt catcatagat tacttccttt tcaattatta
    17101 taattatttt atattaaata ttacattaat ctattgcgct agttaaaatc atatttcaat
    17161 taaaataaat aactctttat aaaaaacaat tatttagata gaaaaatatt tacgaacttt
    17221 aatagtcttt gttaactttg attttattta agttattata gactcgctaa ttttataatt
    17281 gtagatttag ctactttatg ttcttttatt gtttatgttg ttgatttaga agttatttta
    17341 agtacttatt ttaattgaag ttgtttgttt tttaaaatcc tttgtgtaat ctttattttt
    17401 ctagccataa taaaaatatt tgtatttgta tcactatata taataatatc atgacaaaat
    17461 atttattgtc taactaatta taatctattc aaaatgaaaa tgggaatgag atattattac
    17521 aacaagtcgt attattatta aaattgatta aataattaaa tgattaaata attaaatgat
    17581 taaatgatta agtgactgtt atataacaat ttgtaatttt atttaacagc cacttaggta
    17641 tttaagagca atggaattga gtgtatatca aattataata atttcctagt aaataattga
    17701 aaaataaggt aatccatttt tatatttgat atctggctta ccttgaataa gtggatatag
    17761 ataatccagt gcttcgtcag ttatataatt acattcttca tttatccact ctttagggaa
    17821 atactttgtc ttgttagcta ttttagaaat atcagtatga ccaacttcat acttataagg
    17881 attattgcat gttctattaa tgatagccat aactcctgta tattcttttt tagatagctc
    17941 tatcccatat gcaccaacgt cagctgcttg ttttaaatca gtacctgaag ctaaatgcat
    18001 agcacatctt tgtattacac caagttctaa agtttttact cttgttgtaa ttgatgatga
    18061 tactataaga ttctttagat agttgctcac tccacctaat tgagaatgac caaatttatc
    18121 gtgagcagaa gttttttgtt cgcatataaa gttgccgtat ttatctctta taccttctga
    18181 tgcaactata taagccttat ttttttcttt aaatattttt ctaacatctt ctataaactt
    18241 atcttcatag aaatcaattt ctggaaggta tattaaatca acaagtggag tatcattaag
    18301 tctagctagt gaagaagaag ctgctaacca tccagtatct ctacccatag tttctaatat
    18361 aaatatccca ttatttttat atatagaaaa atctaagtac gtttccatta ttagtgtaga
    18421 aataagcttt gcagcagaac caaatccagg acaattgtca gttcccaata aatcattatc
    18481 tatggtttta ggaatcccta taaattttat tttacaagta gtgtttttgc agtattgaga
    18541 aaatttcatt actgtatcca tagaatcatt tccgcctatg taaaataatg tatctatttc
    18601 atattcatct agtatattta taactctatc ataatcataa gaatttatcc tataatcagc
    18661 taatttgtat ctacaagaac caagaccaga tgaaggtgtt tgctttaaat ttaatacatt
    18721 atctttgttt aaagagtctg aatctattat atttttattt aatatacctt caatcccatt
    18781 gataccaaaa tacactttat caaagtattt agtttctaaa tttttttcat aaataccaat
    18841 tgcagaagca ttgataacag ctgtaggacc tccagattgg gcaataatac agtttctcat
    18901 tttacatctc cctttcatta atattgttat gaatgatact ttttattgag caaatttata
    18961 tttatagtct gattatatat taagtgaact agctatatca attgagttgg tatagataat
    19021 aaataccata agaatgtggc aaaaaactaa ctttttatat acaattaatg tctataattt
    19081 accacaatat aaaggtactt ttaaggagta gtcaaagtgt tgtgaaaaga aaaatatcag
    19141 tgtaatatta atttacaaag caaaataagt tcacagttca caaaagcaga ataagttcat
    19201 aaaagaaata catacagcaa taaaagtaat actaaattaa aaaaattaaa aaatatcaaa
    19261 aactaatatt taatattaat taaagtaaaa tatggaggga cacaaatatg aaacacataa
    19321 aaatagcagc aggattggca catgtaaatt atggtcatat atcagccgta gttaaggaag
    19381 caatggatgc aggggtagac tatgtacatt cagatgcagc agatatgcat gacttaaaaa
    19441 atatgcaact tatgggtggt catcaaataa tagaagcaat aagaccagtg acagataaac
    19501 caatagaatg tcatatatac acaagagatt gtgatagatt atttatagaa aaaatagcag
    19561 cagcaggatg taacttgttg ataataccag ctgaacattt cttaggggct cctcttgcat
    19621 acataataaa ttactgtaga gaatttggta tgaaagtagg attgacagtt ggatgttata
    19681 caccactatg ttttgttgat gaagctatat atgatataga cagattgcaa atagttgttc
    19741 atggtgttga tgaaactgat ggaaaagata actggggatg gagaaagagt gctgttgact
    19801 tagtaaaaag agctagaaaa atgatagacg aaaagaatcc taagtgtgaa ttagcaatag
    19861 atggtggact aagagcagac aatatggagc cattgataga atgtaatcca gacgttgtaa
    19921 tattatcttc agcaatattc aaagataaag atggtatagc agcaggtgtt aagaattgta
    19981 gaaaagctat agatgaagca gctacaaagt ttggtttaga ataagataat aaactatgat
    20041 aaaagagatg ctttcttaat gttttaagga agcattaatt ttatgaaaaa ctacaggaag
    20101 ttagactaat ttaattgatt ttttatactg aaagaatata atcattggag gttattaaat
    20161 gaaaaaagaa atgataaagg attatttact gataacagta tcagcatttt taatggcatt
    20221 agcagttaat tttttctttg cagaacatac attagcccca ggagggataa caggattatc
    20281 agtggtactt agtacatttt tatcaatgcc tgttgagaat atatcattag gaatatctat
    20341 gccactttta gtaatgggat tattattttt agggaaaggt tttggaataa aaacattata
    20401 tataactctt atgggtccat tcttcttaaa agtaatacca caaactcaca taactgacaa
    20461 cctattagta gcaggagtga taggtgggtt attggtagga actgctatag gtatagcaat
    20521 aataagaggt tgtagtactg gtggaactga tttagttgca atgcttatta ataaagtaat
    20581 taagtttctg aagttgccag ttatattatt tatgctagat ggatttataa tagtagcgtc
    20641 tggaataata agtaagaatt ttatgatatc tatatattca ttgatatctt tattacttat
    20701 aataaaaaca ataggatttg taactacaaa gtttggtaag aatactgaag aaattaataa
    20761 tataaatagt acttgcactc aataaaaata ataaaactta ctttaaatat aaaagtttaa
    20821 ataagaagtt taaatgtaaa aatggaggtg tctcaaaata aattttttag ttcacgaggc
    20881 actttttttc tatgaaatca aatatatttt tatcatgtag acaatgaaaa ctaggcttat
    20941 ctttatgttg agacaagcct agttttgttt atttatagga taatatttta tttttttcag
    21001 aatacaattt ttttataatt gtactaagtc tggaaatatc attcttgctt aaaagagcat
    21061 ttatatttat cagttcagca ttatgttcaa agtccacagg agcagtggtt agtattaagt
    21121 ccacatcatc aaagttcgta aatgaaatag aagatagagg tatagattta actatattaa
    21181 tctgattaaa ttcaaaactt agtttttgga ctaataagtt agatacacca tcactacaat
    21241 gacatattaa aatagtattt agaggagatt tacttctatc tattgctgat atcaaatata
    21301 aagcaagata ccctatttcg tcatcattaa cttcataatt catcaactgt ttaataacag
    21361 tattagattc tttagcagcc ttatatgtat aagggaatgt cctctttata tcatttaata
    21421 tgggatttgt aatagtaaat ccatagttta ttctgtgtag aactgattta aggtgattag
    21481 caagagactt tattaatatt aaatcatctt ttaagtttaa atttagtata tttccccaat
    21541 gaacagctat tgcttcagat agattatact ctgtaatttc ttcattttta agaggttcaa
    21601 gagtagtagt tttctttatt gatttgagca atccttctac atgaagaagg atataacaga
    21661 cctcttcact aataaattct acatcaaaag cttcacctag gttagaacat aaaatttttg
    21721 atttttcaaa gtattcatgt tccaataatt ctctaaagaa accttcagat aagttaatat
    21781 atcttttaat tgaaatcctc ctaatacata tagctatatg tattaagaaa tttatataat
    21841 actcatcact aaggttactt ttttctactt gcaaagtttg tagagttatt tgagataact
    21901 tctcaaaatc atagttaaat aactccttaa atatttttgt tgatggagta tctttacatt
    21961 cattagattt tgagaataat atttctttaa gctcagtata acttttatta acagtagcaa
    22021 gctcaaaaat tgcttttcga atatctttct cttttccctc taaatatact ccattattgg
    22081 tttttctaac taatgttact ttaaagtttt gtaaaaaatt ttgaataact accaagtctt
    22141 tatgcacagt agctctgcta acatgtagtt cattgcaaat atcacgaatc ctaaacttag
    22201 aatttttaat aaataattta cttagaatat acctttttct atcttcagga gaatatgcat
    22261 atatacaatt tgatttatca ttgatatctc taataacttt aagttttata tcttcattac
    22321 ctagaataat aactcctgaa ccagtttttt tatccaggga taaattaaac tctaaaagcc
    22381 attcatctaa aataacaagg tcattacgaa tagttttatt actaacattt aatttttcgg
    22441 caatagtatc tacagtaata tagtctgagc tgttgagtaa taattcagca atatctaata
    22501 atcttttgtt caatgcaaac cctcctaaat cttagttact atttattaac cttggaccaa
    22561 tctctttcaa aagtattggc accatctact gtaagctggt taaaagaagc ttttttaaat
    22621 aaatcatatg ttacagctgc ataatcacat ccacaaagag atgcttgagt taaatggaat
    22681 acatttttta tagaagctgc aacaacttta gtattatatc catttattga tataaggtca
    22741 catatttctg taaggttttt tataccatca ttcccatttt catcatttct acctataaaa
    22801 gggaataggt aatcagcacc tgcatttata gctaatgatg cttgatttac attgaaaatc
    22861 aatgtacatg cagtttttac acctttacta tttaatatgt ttataagttc taagccaatt
    22921 tcagagaaat ttatttttat tatagtgtct ttatttatat ttaatatctt atcacatgaa
    22981 ttaattaatt cgtataaatt atttccgatt acttcagcag ttaatatttc tggatttaga
    23041 ttagtacatg tctcaataaa ctcatagaag tttatatttt ctttaagata catagatgga
    23101 ttagctgtta ttcccttaaa tccacacttt aaacactttt gaatttcatc aagattagca
    23161 ctgtctatca aatatttcat aatttaattc tcctttaagc ttatttgtca taaaaaagtg
    23221 tatatactct atttttatta ttaagtcaat tatacaataa gattgattat aaaattataa
    23281 catgggtact taataaaatt tacataatga tatggtaata ataaaacatt gattgtctaa
    23341 taattgccat aagctttagt aaataagagt taacagtgaa ggtattgcct taaaaatcct
    23401 atgatatgta taatttgaat atgaaggcaa taatttaagg gaggaaaaat atgattaact
    23461 tttatcaacc atcaagaata aattttggac aaggaactat aagtgagtta cctagaataa
    23521 taggaaaata tggaaagaaa tgtttgttag ttactactcc aaatgtagag ccattagaca
    23581 agctatataa aagagttaca ttggatttaa tagaaaaagg tatagaagtt gtacactttg
    23641 atagagtaga gccaaaccca actgtagaat taataaatga agggtttgaa ttagctaaaa
    23701 aagaatctgt agatgttgtt ttagctgttg gtggaggttc aagtatagat actgcaaaag
    23761 tattagcatt gacttttggt ttagaaaaaa tagattggga ttatatgttt agtacatata
    23821 caaatccatt taaaatatac gataaggtaa gtgaaaaaga attaccatta atagctgtat
    23881 caactacttc tggaacagga agccaagtta cacaagcagc agtaatatcg agaggaagcg
    23941 agaaaaatac aatatttcat caaaattgtt tctcaaaaga atgtatagtt gacccagagc
    24001 ttatgctaac tcttccagag agaattactg catcaacagc atttgatgcc tttactcatg
    24061 cgtttgaaag ttatataaat ccaaatgcta atgactttac agagataatg tctgaaaagt
    24121 ctatggaatt agtcattaac tatttaccta aagtaatgga agataaaaat aatatagaat
    24181 acagaacaaa gttagcagta gcagatacat taggcggaag ttcactggca aatgctggag
    24241 cagcagcacc gcatccacta agtgaaataa taggaggagt tacacatatt cctcatggcg
    24301 aggctttagc tatagtgttt ccaaaatttg cagaaaagtt ctataaagat aatgtagata
    24361 aatttgctaa agtagcgaga atgttaaacc aagaattgga aaaagtggaa gataatgaag
    24421 cagctaaaac tttaggaaaa gaaatagaac gttttttaaa gaatgtggga cttaagacaa
    24481 aaatgtctga gtttaatgtc acaaaagaac aattagaaca aataacgagt tgtccagttt
    24541 taggattttt accttttgga aagaaggaag accttgttga aatattagaa aaatcttatt
    24601 aggatgtgat acaaatgcga tataaattaa tttgtaccga tatggatggt actttgatgg
    24661 gtaaaggatt tgaagtatca gaagaaaata ttaaagcatt aaaggaagct atggaaaagg
    24721 gcattaaggt agccttagtc acgggaagac cttataatgc tataaaatat tttacaagtg
    24781 ttttaggaga tgatatatat attatatcaa ctaatgggac atattttaaa ttactgggat
    24841 acgagtataa gaaggttttg agtaaagaag ctctaaagaa aatatataca ataggtgaaa
    24901 aatataattt aaataaacat tttaaaggtt gtaaaatagt tatatccaac aatgaaatag
    24961 gtcaagaaca tccatataga ttaataaata gtaaaaataa agaagaagac agaattgaaa
    25021 taatagaaaa tgcaagttgt gaaactttat tagaaaaagc tgataatgag attttaaaat
    25081 gtatactttt ctcagaaaac gtagagtctt taagagaagc aaaagaagaa tttaaaaaac
    25141 aagaagattt agaagtagta agttctggaa agataaattt tgaagttatg agtaaaggta
    25201 cttcaaaagg tattgcagtt aaaaaatttt gtgatatatt aggtatagat agcaaagaag
    25261 ttatctgtat aggtgataat gaaaatgata tatccatgat tgaatttgca ggtcttggta
    25321 ttgctatggg gaatgctaca gatgaagtaa aatctatagc agactttgta actgatacta
    25381 atgtaaatga tggtgtagca aaagctttaa gaaaagtatt atcataaaat tttataatgt
    25441 ggagagtgtg ttatgtttaa tatatttaag aaaaaagaaa ataaggttga gaaaatatac
    25501 agccctttta agggaaattt agttaactta gaagatgttc cagatgcagt ttttgcacaa
    25561 aagatgatgg gagatggagt agcaataata ccaagtgaag gtaagttata ttctcctgtt
    25621 gatggtgaaa tagtcagtgt gtttgataca aagcatgcaa taacgattaa gagtaatgat
    25681 aacacaaata tgttgataca tgtaggttta gagacaatgt cactagaagg aaagccgttt
    25741 gatgttaagg taaaggcagg agataaggta aaagctaaag atttaataat ggaagtagat
    25801 ttagatttta taacgtcaaa tgatttaaat ataataactc ctgtagttat aatggatgat
    25861 gctgattctg ttaagagaac agtaaaagat aggatagaac ctggaaatgt agatattaat
    25921 agcataataa tggaagttga atagatataa aaagggagtt gtctcaaaat ttattttact
    25981 aatcaaattt taagttcaac ttcctttttg gatgtgtttt tttgatattc tttttttctt
    26041 ttgcattatt tatatacaaa ctcaattttc atttcttaga ttcttcaaaa agtaaatcta
    26101 ttatttattt catcatattc tacaaaagta tttcataaca agttagagca atttcatgaa
    26161 aatatcatca ttataatgaa aaaaagaaaa tgttgataca gatagtatct tttgttgttt
    26221 tttgaataaa acagaaattt taaagtgaac tttaagacta ttcttattca gaaagtgatc
    26281 ttcataaaaa tgtattttta taaatttatg ttgtctgtaa taatttaatt atttataatt
    26341 aaaagttgtt atttctatga ataaattact ttatatttaa tcaaatgtta taatacaatt
    26401 aaaatgtatt aatatccaat aacagttagg gggattctaa tgattaatat tgctatatgt
    26461 gaagataatt ttgttgatag aggtatatta aatgactact taattagaat aatgaatgaa
    26521 aactcatata atataagttt attcaaaagt ggagaagagt tacttgataa ttatccagat
    26581 gacttagata tattattatt agatattctg atgaaaaaaa taaatggaat agatacagca
    26641 aaaaaaataa gagaatttga tagtaatgtt gaaattatat ttataacatc attattagaa
    26701 tatgcgttac aggggtatga agtaaaagct tttagatata tattaaaacc aattgaatat
    26761 gaacaactgt caattaatat catacaatgt ttagatgata ttaataataa agcaagtaag
    26821 tttgtatata taaaatgtaa tagcgacata gtaaagatag atattgagtc aattttatat
    26881 atcgaaaaaa tatcaagata tatttttgta tatactgaag atcaagtata taaatctaaa
    26941 atgaatttaa aggacataga gaaaaaattg caacattata aattttatag atgtcattta
    27001 ggttatttag taaatgtaaa caaaatagat tgtttaggct caagtgaagt aagtatatat
    27061 aatcatagta ttccagtaag taggggaaag aggaagatat taaaagagtt tataattgat
    27121 tcgaaagaaa agagtttata attatgtatt tgtatgagat atggattatt ataaacttca
    27181 tttcattgat agtagacttt ttaattatta ataaaatttt tacttatctt agcaagaaga
    27241 aaacatctag aaactttatg tttatcagct ttcttattgt tataattatc ttattttttt
    27301 taaattcaaa ttctaataat gaacccatga tatatatttt ttttagcata attttgtatt
    27361 atataaacta tgaaactagt ttagttaaat caataatagt accatcatta tattggataa
    27421 tatatctttt cttaattaaa tatatttctt ctgttttagt tattaatatt aactacacag
    27481 aacagcaagt attaaaaggt gatatatttg cattagagat gatgctatta gcatatattt
    27541 tcttaatttt tttagtgttt ataataaaat ttgttgttac aaaaaataat tttataaaaa
    27601 atatacattt aatatttttt acatttataa atatagtgag ttttttatta atatttagag
    27661 gtgcagccaa agatataaat gtaaattttg tagataatat attcataata attataggtt
    27721 ttacaattgt gatatttaat ttatatttat tatcaatgat ggataaactt ctagaaagta
    27781 gacgaattga aatagaaaat aaatttatga aagaaaaaat agaaattgaa tataagtata
    27841 taaaaaaact gcaatatagc aatgaaaata taaggatttt aaagcatgat attaaaaatc
    27901 accttatttg tataaggaat atacataata ataaagaagc atttgataaa tatttggata
    27961 gtgtcgatat tgaagttaat aaaataaatg atatatatga cactggtagt ataattgtag
    28021 atgctatatt ggatgaaaag aaaattatat gtgacaatta taatataaag ttatatgtaa
    28081 aggcaaattt tacaggatgt gatttcatta gtgcatttga tttatgtaca ataatgtcaa
    28141 acctattaga taatgcaata gaatcaaatc ttaaagtaaa aaattcagat tgtagaaata
    28201 taactgtaaa agcaaattgt gaaaaatata tgctgataat aatagtcgaa aataataagt
    28261 ctaataaaat aattaaatct aaacagagta taattactga taaaaaaaat aaaactattc
    28321 atggattagg attaaaaagt gtcaaaaagt ctattagtaa atataatgga gatttatttg
    28381 tggaatataa tgatgaaaat tttaaagtta aaataataat accaacaaat atatgacgag
    28441 ttatgcacga aaaatgacaa tctatgtaca atatgttgat aaagtaagat gcataaacta
    28501 ctatgtaatt acaaaggggg gtaataaata tgataaaaat aaaaaaaata acagataagg
    28561 aaaaggttgt tgtttcagct caagcaaaaa aaccatgttc atgtgcaggt caagtattaa
    28621 agtatccacc tgtaagtaat tcttgtttat atgattatga tgactataat caacctagat
    28681 ggtatgaggc acaatgtcaa taagtaatac taaagccact tttataatat aaaggtggct
    28741 ttatctataa aatatgtctt tattgggagg agaatatgaa ttataaattt gatgatagtg
    28801 taaaactttt aaaaaatgga gaatatgtaa ttcttggtaa tacaaagaat ggtcaatgga
    28861 ttaaaatgac aaattatatt tataatatat ttaaaatagg aatagataac tctttagata
    28921 taaatgaatt aaaaaaatgt ttagaagatg atgaagatag acaatttata caaaatcttt
    28981 atgagaatct attagacatg agaataatat atgatgaaat taataatgta gcttttcaaa
    29041 ataagatagc ctctcttgaa ataacaaata gatgtaacct taaatgtgtt cattgttgtg
    29101 tagatgcgga taatcttata agtgaaaaag aagagctgtc tacaatagaa cttaaggatg
    29161 caataaaaaa aatagttgaa tggaatccta tgaatattat gttaagtggt ggagaaccca
    29221 tggtaagaag tgattttttt gagatactaa catacttgaa gtctatatat aaaggaaaca
    29281 taatagtttc aacaaatgcg actctaataa ataaaaataa tgtagagtta ttaataaaaa
    29341 atgtgaataa gctagaaatt agcttagatg gagtaaatga acaaacttgc agtatagtaa
    29401 ggggaaaagg tgtatttaaa aaggtaattg atagtgttaa tcttataaag agctttggtt
    29461 tcgataatat aacactatca atggctgtag gtaataaaaa tgaatattta gaagaagaat
    29521 ttaaagatct atgcaaaaac ttgggagtag ggcatatgat aaggcgattt ggtccagtag
    29581 gaagaggaaa agaaaatgaa catttatttt tagataataa caaagatgaa gcttatatac
    29641 ctgaaaattt tttaaaggat aattatgatg gaaatatggg tgtaacatct tgtaaagcag
    29701 gtatttatga aatatttatt agacacaatg gagatatata tccttgtcca tcatttatgt
    29761 cagatgaata taaaattaca aatttatgcg atatacaaca aataagtgag attaaagata
    29821 aatatataaa taaagtggat gtattttcta tattagaaaa aataaatcca gaaatttata
    29881 aaaattgtat agattgtaat gtaaatttat tttgttggac ttgtccagga gaaattgaag
    29941 aactaaaaga tagtaaagca agtttattta ataagtgtag taaaataaaa aatattttat
    30001 ataaaagagt gtgggggaat gatggagtat gttagcaaca ttttggatta caaatgaatg
    30061 caacatgaaa tgcagatatt gctatgaagg taataataag aataataaag ttatgtctaa
    30121 aacgttgata gataaatcaa taagttatgt attaaatcaa tctaatagtt tggatgatga
    30181 tgaacttgta atacaaattc atggtggaga accattttta gaatttaact tgataaaata
    30241 cataataagt gagtttaaaa gcgaaaaatt taataaaaat ataatatttt caacaactac
    30301 aaatggaatg ctcttgaata atgatatttt agattttata caattatata ttgcagaatt
    30361 aaccataagc atagacggtg ctaaaaatac acatgataaa aatagacaaa gtataaatgg
    30421 aaaaggtaca catgatatag ttatagaaaa tagtagaaaa attttaaaaa taaaaccaaa
    30481 tgtaagagta agaatgacat ttgatacaga gacagtaggt aatttatcta atgatataaa
    30541 atatttaata agcgaaggat ttagaacgat tgttccagtt ccagatatgt ttgagactag
    30601 atggaacaat aatcatatag aggttttgaa aaatcaaata taccaaataa aagagattat
    30661 aggtaacagt aaagatattc ttgtaagcct cacagacaca aacatatata agtataaagg
    30721 tgtttgctct ggaggaaagt taagctttaa tatatgtcca gatggatata tatatccatg
    30781 cacattagca gttggaaata aagattttag tataggaaac atatatgatg gtatagataa
    30841 aactagtaga gacaagtttt tgagtaaatc atacctagat aataacgatt gtaaagggtg
    30901 tgacttaagc aattcatgtg atggaaatag atgtaagata ataaataaat taactagagg
    30961 ttcgtatttt ttaccatcgc caataaattg tgctatacaa aatataaaac atgagataaa
    31021 tataagtaaa gaaattgtat tatagttttg tagagttaag gaggaatagc ttgaaacgaa
    31081 ttaaggttgc attaattgac tcaggtatag atattaatca taaatattta aagaataata
    31141 tattgtatgg caaatcatat atttataaag ataataaaat agttgtctca aatgaaattg
    31201 aagatattaa tggacatggt acatcatgtg catcaattat aaaaagcgaa tatgaatatg
    31261 ttgattttat aataattaaa atatttgata gagatggatt aacaaatatc aaagtactag
    31321 aagaatcatt aaattactta ataagtattg atgtaaaaat tataaacttg agtttagcaa
    31381 tacatggaga cattaattct tgtaaagact tatataaaat atgtgaaaaa ctaaagaatc
    31441 aaaataaaat tatagtttct tcccttgcaa attataatta tatagaagag agctatccat
    31501 ctatatttga taatgtaata ggagtaaaag gaaatttatt aaaaaaaaga tcatcatttt
    31561 ggtataattc agaatataaa tatcaatgtg ttgtagacga tagtgataga atagttgcag
    31621 atttaggtaa cagttttaag ttagttgaaa aaagcaacag tttttcggct gctaaaatga
    31681 gtggaaaaat agctaagata atagcaaata aagtggatat aagttttgat ggattaagta
    31741 gaatactaga agaattgtct gagtgtacaa attggaaaga cgaagatcta tatactaata
    31801 agagaagaat agattacaaa cagtatttat atgaaaacaa taatataaat cttaaccgag
    31861 tattaaaagt gttggttgat aatcttaata ttgggtgtaa tgtggatatt ttatttgata
    31921 gtatattatt tagtaaatat ataggtctaa aaaataataa ttgtattgaa atcctaaaga
    31981 aactagagaa tgaatttaat ataaagattg attacacaaa agtatataaa gaagatctaa
    32041 tatcaatata tactttaact aatttgattg aaaaggagag tatatgtgaa cattttagaa
    32101 actgtaaata tctccaaaag ctatagtata ggcgataaaa aatcatatat attaaaagat
    32161 ataaatataa aaataaaaga aggagaattt atatctataa taggtcctag tggaagtgga
    32221 aagtcaactt tacttcacat attaggggga ttagaaaatc ctactactgg tattgtaaaa
    32281 attataggaa aaagtatata tgatttatct gagattaaat tggcagaata tagaagaaga
    32341 aggtttggat ttatatttca acaatttaat ctaataccag tacttaatgt aagagaaaat
    32401 attgaaatgc cattaatgct agatagagct aaaattgata ataggttcat aaaagaaata
    32461 attgagtttt taggtttaga agaaaaagaa aattactatc cgaatcaatt atctggaggg
    32521 caacaacaaa gagtagctat agctagagct ttagtaagta agccagatat tatttttgca
    32581 gatgagccta ctggaaacct agacactaaa aatagtgatg aggttataaa acttttaatg
    32641 aagtctatac acaagtataa tcagactgta ttattaatta ctcatgatta taaaattgct
    32701 aaaaacacag atagaacaat taaaatacta gatgggaaac tagaattata aatgatacaa
    32761 tgtcaagagg gtaataaatt gaaaaatact attaatatga aaaagtttct atatctcata
    32821 attcaaggta ataaaaagct agtaattata tcttttctta tgatgatagt agtagcaatg
    32881 atagatttgt atatacctca aataataagg aatatattgg atatagctat aaaatcaaaa
    32941 aatattacta tgctaataaa actaatatta caatttatat taattaacat aatattatct
    33001 aatctagaac tatttctgga atataattat tctaaaatga agaagaaaat aactacaaaa
    33061 ttgaagttaa aattattgag acatttatca aagttagacg gacgtttttt ctctgaaata
    33121 aaaacaggaa atattatgaa catagtagag tatgatgtaa tgatgttaga cagatttgga
    33181 atagaattaa tattttcatt aattgtggac ataataactg catttatatc gttgtatttt
    33241 ttaataaaga tacaatcaaa acttttagta atagttgtag taattcaaat tgtaatttct
    33301 tttactcaat atatatttac aaagatgatt tctaagtata cacagaatat aagaagtaat
    33361 gctggaagta tatctaatat agtacaagaa tacatatcta atatcatgaa tgttgttata
    33421 cataaaggtg aacttaattt ttttacgcaa tttctatcaa tgcaaaagaa ttttgtaagt
    33481 aaatgcataa agctagatat gattatggct ggaaatgttt ctttatctaa gatattaaac
    33541 agtttaattg ttatttctat atatggttat ggtggatata aaattataaa tggagaaatt
    33601 acgtttggag aattggtagc ttttcaacaa tatacaggta tgcttattgg cccttgtatg
    33661 aatataataa aatctaatgc tattgtacaa caatcattag tatctattaa tagaatattt
    33721 gatttattaa atgaagaggt taatatgaag caagaaaata caggtgaaat atttcagagt
    33781 gggataacta aaaaaattag aatggatatg atagatgtaa gtttttcata tgataaagaa
    33841 gacgtattac ataatataaa tatgtcactg gagagtggta atatctatgg aattgttgga
    33901 ggaagtggtt caggaaaaag tactttaatt aaactaatat atagattatg ggatattgaa
    33961 agcggaagta taagaattaa tgatgtagat ataagagatt ataatttgaa aaatttaaga
    34021 aaaaatatta ctattatcac acaagaacca ttattattga atgatactgt ttttaataat
    34081 ataattttaa gcaacaagaa tattaactat gagctagtag taagaatatg caaggaatta
    34141 gatatatatg attttatagt aagtttaccc aatgaattta atacaattgt aggagaaaat
    34201 ggtgtaaaat tgtctggtgg gcaaaaacaa agaatttcca tagctagagc aatgttgagt
    34261 aattcaagta ttctagttct tgatgaggct acatctgctc ttgataatat atctcaaaaa
    34321 gatttattaa aaagtatgaa aaagttctta aataataaaa tagttataat aatagctcat
    34381 agactatcta ctataaaaga ttcagataaa atatttgtaa ttaataatgg tgaaataatt
    34441 gaatctggaa gtcataaaga tttagtgtct caaaatggat attattgtaa tttacttgaa
    34501 gataaaaaag aatatagtta ctgttgatga ttagatagtc aataaaaatt ctatttttat
    34561 attagaatat attgactatt tttttgtatt ataaatacaa tgtatgaatg aataagtaca
    34621 aggtatgtaa catttattga tatatattta ttaaaatgat acatttattt cagtaattac
    34681 ataaaggagg aattttatga gactatctct agcatatgta aaaaaatact ttggaaggtc
    34741 aatatctata gctttaagca taatcattag tatctcactt ataattacag ttggaatact
    34801 tactaaaaat actcaaaaag caagtgttga tagaataaag tatgaatctg gagattacca
    34861 tgttaaagtt aagaatatta gtggaaatgt aatatcggat tataaaaagc aatttgatat
    34921 aagtatgatg ggtgtatcat cttattacga ttcaagtgaa tttaatggaa aattaatgtt
    34981 aaatttaatt aaatctaatg aagactatat aaaaatttat aattcaaaaa ttctaaatgg
    35041 aagatttcca actagtgaca atgaagttgc tttagaagaa tgggttatgt tgaatttagg
    35101 tctaaaatta gatataaatc aaaatataaa aataaaatta tataatagca aagagactaa
    35161 aaattataag gtagtaggta tactaaaaga tagaccatat tacaaatcta taggaatggc
    35221 ggaactaata cttcctttta cagaaaatat aatcaaagaa acgaatctag aagctcaaat
    35281 aagatttaaa aaagatatat atagaaactt atcaaaacta atgaatgata atcatataag
    35341 tagaaaaaat gtatctgaaa atagaatact attagatgcc ttaggagaaa gtaatggact
    35401 aaattacgat tttatattta catgcattat atcaatgatt gtatctggag tagtaattta
    35461 ttctatattt agaatatcaa taattcaaag aatttcggaa tatggaatta ttagagcctt
    35521 aggagcaaat atattcaatg tatttaatat aatatttgta gagctttttg caatttcttt
    35581 agtatctatt ccaattggag ttttgatagg tattataagt tctaaatata taaccttatt
    35641 tatgggaaat ttacttattg gcaatatagt aaaaataaat aatgtagaaa taaatttaga
    35701 tgtagtgatg atatctataa taacagtact tgtaaatata ttaattataa gttttttaac
    35761 atgtacaaat gtaaataaaa tatcacctat aaaatgtata aagaaagata tatctaaaaa
    35821 aaattctaga gaatcaagta tattaaatac taggagatta ctgaatcttt tacccataga
    35881 atatataata tccttaaaaa atattttaaa aaataaaaaa aattttgtga ttatagtatt
    35941 gtctatgagt ggagggtgta ttatatttat attgactaat tttacaaaca tattggatga
    36001 aagacaacta gaatatacat ctaaagttgc aacagaaaat tcagactata atataagctt
    36061 tgcacctgtt actccattga atataggtat atctgaagaa aatttaaaac aaattagaca
    36121 actagaaggc attgaaaatg ttaaaacatc tcaagttatg tattcaagaa tgatattaaa
    36181 taaagataat ataaataata tgaattattt tgaaaaagta aataagtctg cttataataa
    36241 gaaggcttta aatggattat tagttaaaaa agataacgag tatatattaa agagcaatct
    36301 atatggttat gataattcat caatagatgc tcaaaaatcc tttgttattt caggagatat
    36361 agatttagat aaaatgaata atggaaatgt ggcaatactt tatatgccgg atcctagttt
    36421 atttacaaac attaaagatt ctcgaccagt tcttaattta aaggtaggag atattgtaaa
    36481 attgaggttt agaagtgatt atgtatcaga tgaaaatttt gcaaaattga aagataaagg
    36541 agaatatgtt gaggaagaat ttaaaatagg tgcaatagtt agtaatattt taaatgtaga
    36601 tggttattat gcaggtatgg acagtgcgtc tataataata tctcaaaatc aatttaaaaa
    36661 tattgtaggt atacatagtt atagaatgtt agatataaaa aaaataccaa cagttgacaa
    36721 tatagagctt ggaaaaaaca taattgacat atctagtaaa attcaaggtt cgatagttag
    36781 agactttaca caagagataa aaaatataaa aattatagaa gataataaaa aaattcttat
    36841 atacacaata atattagtcc tttttacaat tagttttata aatataataa ataatataaa
    36901 ttataactta tactctagaa taaatgagtt tggtgtaata agagctattg gattggataa
    36961 taatagtttt agaaagatgc tattatttga aggtgttgtt tatggactta tatctgctag
    37021 tgtgtctatt acctttggaa ttttaggtca atctatactc tacaatattt taaaaggtaa
    37081 tttaatgaat gccaaattta taatagatta taaatcatac atattggtaa taattttaac
    37141 tattattctg ggagtagtgg caacatattt accatctaaa aaagttaaga aattatctat
    37201 tattgaatct ataaatatta ttgaataaaa tcataagtaa aatacattta tgctgtgtaa
    37261 atagtaatta ctaggaaata atataaaaaa taaataaaaa tgataaag
//
LOCUS       DAMJVM010000031        36819 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.031, whole genome shotgun sequence.
ACCESSION   DAMJVM010000031 DAMJVM010000000
VERSION     DAMJVM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36819
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.031"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            77..151
                     /locus_tag="PC523_001629"
     tRNA            77..151
                     /locus_tag="PC523_001629"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:109..111,aa:Thr,seq:tgt)
     gene            complement(299..976)
                     /gene="srtB"
                     /locus_tag="PC523_001630"
     CDS             complement(299..976)
                     /gene="srtB"
                     /locus_tag="PC523_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HDF2475162.1"
                     /translation="MKKLYRIIINIILILVILYSGFNIYSKLSKYNHDTKTSSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSLDDKNVIIYGHNMKNKTMFNNLNKFKDADFFRQNNKIKINLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNSESDYQNYINDITSKSLYKSPIKVDINDKIITLSTCTYEFDDARM
                     VIHGRLI"
     gene            1108..1442
                     /gene="rnpB"
                     /locus_tag="PC523_001631"
     ncRNA           1108..1442
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PC523_001631"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1720..4254
                     /locus_tag="PC523_001632"
     CDS             1720..4254
                     /locus_tag="PC523_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HDF2475163.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSEKRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVSNNVEGNNEQDRLEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKLSDVDSKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEGYGFDKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAYNLIKDDYSELENHEIALKRTRKNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDVEEETKEEVASNNQDIDNFVEDKDITDKEYDSNKEDIIE
                     SENKKSKKKAKLFGFIKKDNEEVEQEEENLNGISSDVILDEPIENNRVKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSLNNESKLEKESRNLEDEKVKEEKLE
                     NEKSVVIPINKKENSNKKSKNSSKDKSREHRKDAKNYVSDKEDSLDDEDVVSKKNRLK
                     ETIIAVVIVVIVGVGYFITVGNNKKNDKEDIPKSSTQQQANNKLTEEEKKAQAEKEKK
                     EAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGIYYTVYAGSLKVEKTAKETAKEYEA
                     KGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLEELK
                     ESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            4375..6366
                     /locus_tag="PC523_001633"
     CDS             4375..6366
                     /locus_tag="PC523_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475164.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNKDTGVKYIYSFSDHKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKLYMGGINKDNEKFIFISGKNRFEVN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCDIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLNDIENETVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKILLNLSKNMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEHRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            6356..6676
                     /locus_tag="PC523_001634"
     CDS             6356..6676
                     /locus_tag="PC523_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475165.1"
                     /translation="MSSKYNVWNFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSDLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYIDEDIDSDIKGI
                     LDEI"
     gene            6874..7743
                     /gene="galU"
                     /locus_tag="PC523_001635"
     CDS             6874..7743
                     /gene="galU"
                     /locus_tag="PC523_001635"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDF2475166.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIHCAKSFIGDEPFAVLLGDDIVDSEVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFNILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            7767..8780
                     /gene="galE"
                     /locus_tag="PC523_001636"
     CDS             7767..8780
                     /gene="galE"
                     /locus_tag="PC523_001636"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDF2475167.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNLIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHIVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLATGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            8892..10826
                     /locus_tag="PC523_001637"
     CDS             8892..10826
                     /locus_tag="PC523_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HDF2475168.1"
                     /translation="MKKSLKQYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKIIDGKTYYFNSDGVMQTGWQEKNGKKYYLGADGLSV
                     TGWQEIDGNKYYFDKTGIMQIGWQQIEGKSYYLDKDGKMLTGAQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTDNLPNETVEEMKLLAPKTVILIGGENAISSKLEQEIKTIFNAETKRIAGQDR
                     YQTATRIAEELGSREEIKTAYIVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAISDSVIARMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDADLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMYNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILGK"
     gene            10981..11844
                     /locus_tag="PC523_001638"
     CDS             10981..11844
                     /locus_tag="PC523_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HDF2475169.1"
                     /translation="MVSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPII
                     EPVGPWLEKMR"
     gene            complement(12052..12933)
                     /locus_tag="PC523_001639"
     CDS             complement(12052..12933)
                     /locus_tag="PC523_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2475170.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPTKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYF
                     PPAIKNFKSFVIKNAQL"
     gene            13346..14194
                     /locus_tag="PC523_001640"
     CDS             13346..14194
                     /locus_tag="PC523_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDF2475171.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGTEEDRTEAQREKEKNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTADDLAPIPADKIVSIQVCDVLENPYEKLRDESLHDRLA
                     PGEGFGNTEEFVKIIKEHNILPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            14273..16195
                     /locus_tag="PC523_001641"
     CDS             14273..16195
                     /locus_tag="PC523_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2475172.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YIERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKIAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEIHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVLGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPISCLANPILGHEAEFKSAEISKEVLVVGGGVGGML
                     ASWICAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDAVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHDVTVIELREEVGADVISEHRKFLMNDFENYNIHTVTSAKVA
                     KFYDDGVDYLLADGTEHDLKGFDSVVLAMGSRNYDPLSEVIKELVKETYVVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            16277..17140
                     /gene="aroE"
                     /locus_tag="PC523_001642"
     CDS             16277..17140
                     /gene="aroE"
                     /locus_tag="PC523_001642"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDF2475173.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFEKLDLDYRYLAFDI
                     TVDKVKEALSAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENEVEVKGKKVTIMGAGGAATAIQVQCALDGAREISIFNAKDDF
                     YKRAEQTVENIKKEVPKCIVNLYDLEDTNKLYEEIESSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPRKTKMIEDAESNGCKAIGGLGMLLYQGAESFNLYTGLEM
                     PVKEVNELCFK"
     gene            17342..18559
                     /locus_tag="PC523_001643"
     CDS             17342..18559
                     /locus_tag="PC523_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2475174.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYIFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAILIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            18795..20660
                     /locus_tag="PC523_001644"
     CDS             18795..20660
                     /locus_tag="PC523_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HDF2475175.1"
                     /translation="MENVFKRLQEFDGYDGYKESFEMNYLCIYENIPLREQVELANNL
                     IDGILNMYKSESNEIYLLEDSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLDVENLREVVDTFSKSGEYVFYLSNAIKKIEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEYGYKDTKYQRLLMNYIISIVDLNEYLE
                     KRDLDREKINNLSLLICNYLLSVEFKYIGNKLELVNRFLPIVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKVEFEKEINDILLSEKWKSIYFEALKDASGKTEDMIKMSEIYNVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLDFFEKTFKVDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKVVKEAYEKEKNIILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSITV
                     AGSRFRNREYLEKELEKSKIYYLIREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(20838..22202)
                     /locus_tag="PC523_001645"
     CDS             complement(20838..22202)
                     /locus_tag="PC523_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2475176.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDISIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEVDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGISVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKEQASKIKDKSDVKIPSKF
                     KIIK"
     gene            complement(22380..23723)
                     /locus_tag="PC523_001646"
     CDS             complement(22380..23723)
                     /locus_tag="PC523_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDF2475177.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(23796..23897)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            24226..24879
                     /locus_tag="PC523_001647"
     CDS             24226..24879
                     /locus_tag="PC523_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2475178.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLAKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     gene            25020..26291
                     /gene="brnQ"
                     /locus_tag="PC523_001648"
     CDS             25020..26291
                     /gene="brnQ"
                     /locus_tag="PC523_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2475179.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAIFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            26493..27680
                     /locus_tag="PC523_001649"
     CDS             26493..27680
                     /locus_tag="PC523_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HDF2475180.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKVIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINAEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNDYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISKKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSLAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(27910..28356)
                     /locus_tag="PC523_001650"
                     /pseudo
     CDS             complement(27910..28356)
                     /locus_tag="PC523_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            28401..28880
                     /locus_tag="PC523_001651"
                     /pseudo
     CDS             28401..28880
                     /locus_tag="PC523_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28882..29178
                     /locus_tag="PC523_001652"
     CDS             28882..29178
                     /locus_tag="PC523_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475181.1"
                     /translation="MSEVVKKEVQVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVVATAIMTIVISRRGFPLLYTKTKM"
     gene            29573..30763
                     /locus_tag="PC523_001653"
     CDS             29573..30763
                     /locus_tag="PC523_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2475182.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQEDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIIQKPVYYPFEAKVKSNNREVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNKSEDWLNELNEYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMGRLKTT
                     FENL"
     misc_binding    30897..31152
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            31211..32212
                     /locus_tag="PC523_001654"
     CDS             31211..32212
                     /locus_tag="PC523_001654"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDF2475183.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEDIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            32316..32705
                     /locus_tag="PC523_001655"
     CDS             32316..32705
                     /locus_tag="PC523_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HDF2475184.1"
                     /translation="MDFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIADKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDVIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSTIIENVNFSIKDEEE"
     gene            complement(32870..33016)
                     /locus_tag="PC523_001656"
     CDS             complement(32870..33016)
                     /locus_tag="PC523_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDF2475185.1"
                     /translation="MLLYGTNKKTILDRLGHIDIKIAINKYSHVLEEVKKEISENLSK
                     ILFK"
     gene            33317..33772
                     /locus_tag="PC523_001657"
                     /pseudo
     CDS             33317..33772
                     /locus_tag="PC523_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894433.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
     gene            33776..34606
                     /locus_tag="PC523_001658"
     CDS             33776..34606
                     /locus_tag="PC523_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2475186.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKVDVITCPERQFSSKNEEKSHKIPRVNSGGYDLLIELHLNASNGQGKGSEVLYYSN
                     KGLEYATRVCDKLGTVFKNRGAKLDKNLYILNSSKPTAVLIESFFCDNKEDYESAKKL
                     GYEGMAKLIVEGILNKGVANNEVKQMYKHTIVYSGDDKVSAEILGLYYKRAKENYLVS
                     DIKDYEPHQTQNLYVIGGETCNKMKEMSNTTGEKFTEIYSDDVWSTMDKAIEFVKEKL
                     "
     gene            34881..36536
                     /locus_tag="PC523_001659"
     CDS             34881..36536
                     /locus_tag="PC523_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475187.1"
                     /translation="MNLDFHYYGTYLAAKVAGYNDTDAKTIAYAAQYVDESDKSMILD
                     DVNFTLPTIQTNLEFKKYYADLASWGYKWDFESLNEIKKVWIPFHFLPGNLNNQYKYN
                     GVKESKCLTTSWKFKDGDDQKFRLMCLPNSETVSAIINDLIYFHSTEEYKLQFIGMRM
                     HVLADTWAHMYFIGKPEWYINDVKEFIAEESKYIEETKWTKAEEYRNNGWHSINFTGT
                     PDIGGYESISYLGHGRMGHIPDYGYLNYRYIPNWSSTDDKVKIEKNNQDNFFKAFCQM
                     VYALKCIKNGNDFNINQYDNLTAQQKTEVSKVIATRKNDQSEAWKKAIQALGYNPLEK
                     FDKNKWKNEFIKSSNKEKTDYYYFNLVAKKHVDYVTDFLEEKRLSLEEMFNGYCKFKN
                     FDDTQFAKNRNRVTKIILRGAYIVDAIQLVYDGKYKTPMHGEPFGGTMVSLDLDTDDY
                     IVKISGSIGLYEGGEPYPNSPTRTIGKITFLTKKGKTITAGHETMFKLYRNFTLEAPA
                     GKQIFALKGSYLIRKLREETSKRYLDCLEIANTKDCSVATSTK"
BASE COUNT        14409 a         4005 c         6380 g        12025 t
ORIGIN      
        1 ttattaaaat atatatgtag tttttagagt gttaattcta aaagttagct ttattaattt
       61 ataatagaat aagtgtgctg gtgtggctca acggtagagc agctgacttg taatcagcag
      121 gttgtaggtt cgattcctat caccagctcc agtaaagact atgtttttag catagtcttt
      181 ttttattgtt taatgtgtct tttattttgt actacatatt tttattagcc taatataaat
      241 tattggaaca aaagatgaca cagtttaaaa ttattgtttt aaactgtgcc tgtgagtttt
      301 aaatcaatct accatgaatc accattctgg catcatcaaa ttcataggta catgtagaaa
      361 gtgtaattat tttatcatta atatctactt taataggaga tttatacaat gatttagaag
      421 taatatcgtt tatgtaattt tgataatcag attcactgtt aaaatttgtt tttagataat
      481 cataatcaga ctctacaata taagcagaaa aaacatcata taaaaattct tttccattta
      541 agtttatttt tattttatta ttttgtctaa agaaatcagc atctttaaac ttatttaggt
      601 tgttgaacat agttttattt ttcatattat gtccatatat tatgacattt ttgtcatcaa
      661 gagatttatt tctataatcc ataaatagtg tacctgaaat agaatctttt ttataaaaat
      721 ctttgtcaag atagtaagaa ttatctttag attgtacaac tggatagttt atatttgtat
      781 tctcaacaga aagccaaaat ttaaaatcac tgttaatctt tgataagtcc tcttttttat
      841 attctttttt ttgaagttct gaggatgttt tagtatcatg attgtatttg ctaagttttg
      901 agtatatatt aaatccactg taaagtatta ctaatattag tataatgtta attattattc
      961 tatataactt tttcaatata tcacctcgag ttaaatgtag tttatatacc tatattacac
     1021 tatttttgaa taaatttaaa tttttagtat aatattactt tgacatgtaa aagaatttgt
     1081 gttaacataa ctaagtaaaa taaatattaa gtaagccaga taatcgcatg taattttaca
     1141 tgaggaaagt cggagctcca tagagcaggg tgctaggtaa cgcctagtgg gagcgatcct
     1201 aaggatagtg caacagaaat aaaccgccaa cattgtggta aggatggaaa ggcgaggtaa
     1261 gagctcacca gcagctaggc gactagttgg ctatgtaaac cccacctgga gcaagaccaa
     1321 gataggaata aagagtgctg ctcgtactct ccggtaggta ggtcgcttga gcctattggt
     1381 gacaataggc ctagaaagat gattatctaa tacagaactc cgcttataga cttgcttaat
     1441 tattttgata atcaaaaaca tcagtttaaa ctgatgtttt tttatttgta aatttgtcta
     1501 atctactttt aaaattttga ggaatattgt aatataagtt agtacagata ttaaaaatgg
     1561 tataatataa gatagatata aaaagaaaaa gtaaaatgat gtaaataata tgatatgata
     1621 taagatgtag caaatttagt aaataattat ttaaatatca taatcaaatt agcaactttg
     1681 cacaatgtaa atattacaat aaaataattg atttaaggag tgaaggtttt gagtgaaaat
     1741 aatataataa gaaacactgt tttaaatgtc acagcaagtt ttttaaaaca agagtctaaa
     1801 ataaatgaaa agctagatgg tgttttggaa aagaaatttg aaaaagtaga atttaatgaa
     1861 gcaaaatatg cagagctttt aaaatttaat atattgtttt ataagacatt agcaagaaat
     1921 acagaaccac tgattggcaa atggatagta gataagtaca tacctgaaat agatgaatta
     1981 gaaaaggatt tagaattaac tacagcaaaa tgtagaaagt atgtaaataa ggctatgaaa
     2041 gagggattag actctctaaa agccaatgat ttaaatagtt ttttagctta tgataaaatg
     2101 gaactatcag agaaaagacg acgtttagaa aaggattata aggttttaaa cctatacaaa
     2161 gaccttctca atatatctct tagaaaaata agtttagaaa agaaagaatg tggagattta
     2221 tttcttaaaa atcaagcaga tgctaaaaga gaattaaaaa gagaaatcat attctgtgta
     2281 aacaaaatat tggctagtaa taaagatgta gtgtctaata atgtggaagg aaataatgaa
     2341 caagatagat tagaaataaa agcaactgaa attgaggatt tgcaattagg taaagataat
     2401 tcagaaaatg atatgctatt aaatgaaatt aaaaattcaa aagtaaaaca tgaatcaaaa
     2461 ttaagtgatg tagattcgaa gtttatagat aagctaaata gcttaaataa tgaaagtata
     2521 tctcaaacaa tacacaatga atataagaag ttatgtggat tagaagaact tcattctgtt
     2581 gaaggatatg gttttgacaa agaaattatt aaggattttg cctgtgcaac agttgtatta
     2641 gaatttttaa agagaagaaa tagagacctt atagaaggtg caatgagact gactataatt
     2701 ggagaatttg gtcctgaaaa ttttaaggaa tttgtagact atgtaattaa aaataaaaca
     2761 gaaattagtg aagatacgtg gaataaagca tataacctaa taaaagatga ctatagtgaa
     2821 ttagaaaatc atgaaattgc attaaaaaga actagaaaaa ataaagatat cgatattgaa
     2881 gaatacattt acatgataaa aaatgcagat aaagatattt gctttagaag tagtatttca
     2941 atagaagatg atgttgaaga agagactaaa gaagaagtag ctagtaataa tcaagatata
     3001 gacaattttg tagaagataa agatattaca gataaagagt atgatagcaa taaagaagat
     3061 ataattgaat cggaaaataa aaagagtaaa aagaaggcta aattatttgg atttattaaa
     3121 aaagataatg aagaagttga gcaagaagaa gaaaatttaa atggcatttc ttctgatgta
     3181 attttagatg aaccaattga aaataatcga gtaaaatcag aagaaataga gcaaaatgaa
     3241 ctaaaagaaa taaaacaaga agaaccaagt caacatatag aagaagaacg tagtgtaaaa
     3301 atagaaaaac caataaataa taatttagat gaaaaggtat ctttaaacaa tgaatctaag
     3361 cttgaaaaag aaagtagaaa cttggaagat gaaaaagtaa aagaagaaaa attagaaaat
     3421 gaaaaatcag tagtaatacc tattaataag aaagaaaata gtaataagaa atctaaaaat
     3481 tcttctaagg acaaatctag agaacatagg aaagacgcga aaaattatgt atcagataaa
     3541 gaagactctt tagatgatga agatgttgtt tctaagaaga atagattaaa agaaactatt
     3601 atagcagtag ttatagtagt gattgttggt gtaggatatt ttataacagt aggtaacaat
     3661 aagaaaaatg ataaagagga tattccaaaa tcatcaactc agcaacaagc caacaataaa
     3721 ttaactgagg aagagaaaaa agcacaagct gaaaaagaga aaaaagaagc agaagaaaaa
     3781 gcaaaagctg aaaaagaggc tcaagaaaaa gcacaagctg aaaagaaagc aaaggaaatg
     3841 gaagcatata aagatggtaa aggtatatat tacactgttt atgcaggttc acttaaagtt
     3901 gaaaaaacag caaaagaaac agcaaaagaa tatgaggcta aaggtatttc ttcaacaata
     3961 attcaagaaa atggatacta taaaataaaa ataggtgatt attctcaata tggagaagca
     4021 caagaaaaat gtaatgaact agctaaaaag tctattgata catatatagc tatgtatgat
     4081 aagtactatg attataaatt agaagaatta aaagaatcag ctccttcttt gagtgctgaa
     4141 gaacttaaac aaaaatatga agatttaaga agtgagttaa agaataaatc tggatatcgt
     4201 gaatatgtta aacatttaga taaactttat gaagaaatag ttgaaggtgc ttagagtatt
     4261 ataaaaaagg agaaactatg tctcagaata aaaagtataa aaaaatatta tttattttga
     4321 gacattttgt tcacctttat aaaatactta taattctgtt taggtggtga aggtatgttg
     4381 aaattaggag aaaaaatcat atatgagctt tcagatggat ttgagcttag tggtatcagt
     4441 agatactcaa aagaatataa aaagcttcat ataacaaatc tagggaatat aataatcagc
     4501 aaatcagatg aagttaataa ggatacaggt gtaaaatata tatatagttt ttcagaccat
     4561 aaggaaaaat tagaggcagt tttagaagat tctcaaataa ttgtctatgg aaataacttg
     4621 cctttttaca taaaaagtga aaattcaagt aaaaatgtta cagcaaattt aactatgaaa
     4681 ataactttgg atgaatatga tttaatttgc aaaagccaaa gagagtttat attttatcta
     4741 aaggataata tgactatatt aagtttagat gacaataagc tttatatggg tggaattaat
     4801 aaagataatg agaagtttat atttatcagt gggaaaaata gatttgaagt aaattttgat
     4861 gatatagaaa ggtatatttt agaagataaa agagtgtcct tgaagggata ttttcatatg
     4921 gaaagagagg ggattatagt aaggtctgta tctatattta ataacaatat agatagtgta
     4981 gttccatctg atttaaatga gagagttaaa gataatcaaa aaatagggaa ccttcctaaa
     5041 gattgtgata tagtgttctg caagatatca ggaaatatag atggatttga ttataaaaac
     5101 acaaacatgc ttttagtaaa atatcaagac caattaattt ttataaataa gaaatctaaa
     5161 aagactattg taaagtcagc aaaagataat tgttcaaagt taaatttggg agaagatata
     5221 atcttatatg ataataaaaa tgtatttaac ctacatataa atgataaaaa tagagaaatt
     5281 atgcaaatag atgatttaaa tgatatagaa aatgaaacag tgggatatac attaaaacat
     5341 gctccatttt ttattcaaga agatttcgat agcttgacta ttctaaagtc ttttcaaaaa
     5401 gaaatcatat ctataaaaaa tagtgatata aaagacattg taattaataa agagcttgaa
     5461 aacgaaaatt ctaattttgt agaaacagaa ataaaattta ataatcaaaa aatactttta
     5521 aacttaagta aaaatatggt tcaaaaactt atgcaagatg tatttatata tgcaaagcaa
     5581 cctctactta aagaaaattc tattgaagtg atatataaga attggtctaa agctatgaat
     5641 gatatgataa tatttaattt ctttggaaat atttattata tgaaatctga atttgataag
     5701 attttagaaa aagaattaaa tgatgaaata agaatagaag ttataaattc actttataag
     5761 caaatacaag aacacagaaa caatttagat ttattgtcag catatatgcc aagaatatta
     5821 gaaaatcaag aaatagattt gtttgaaaag tataatacta aacttgatgt acaagtgttt
     5881 aagcagatta aaaatctatt gtctgactta tcatataata taagtagtta tttaaatgaa
     5941 gttgaaaaaa gcttagacaa tatcatcttt gtaatttcag gagaagataa gaagaaatac
     6001 aactatagaa tgttaaaaga aagtgaaagt gcaagtcttg atgtattttt aaaacaagct
     6061 atatcaagat taaatcattt agtagaaaat atgtatcctt attatgtaga tgaaactagt
     6121 agggaaatgt ttaagttatt tgagttattg tggaaaaact atagaaatat tgatgatgat
     6181 agtataaaag aaatattatt cgagagaata acaaatacat atgtatttaa acaattgaca
     6241 ttaaataata gtacaaaaga aagaagaaaa gacataattg aaaaaatata taattctgtt
     6301 gattatggaa ccaataaact agatgaaaat atgtttttca caggaggaat aaaatatgtc
     6361 aagtaaatac aatgtttgga attttaacta tgagttctta ggcttggact caggagatga
     6421 aaaaaataat gaagtttatt atgagattga tttagatgaa attttaaatg aaaactatat
     6481 agatgaagat atagatagtg atttagattc tgatggaaat agtattttag gagaattaat
     6541 atcagagatg gatatagatg aagatgtcag tgttgatatg acatatcaag aactagaagt
     6601 tgacttagaa gatttagatt cttatataga tgaagatata gatagtgata taaaaggcat
     6661 cttagatgaa atttagtacc tgtataagtt ttagataaaa tataaacaat acaatattaa
     6721 cttataaaac taaaggtaga tgaaatttca tctaccttat attatcttat agcattaaat
     6781 attgggataa tatagagttt gaagaaaaag tataaatatg gtatattaat ttggtacaat
     6841 agaaaattta cgaagaaggg tgaaaaaatg cagatgacag ttaaaaaagc gataatacca
     6901 gcagcagggc taggaactag atttttacca gcaacaaaat cacagcccaa agaaatgctt
     6961 cctatagtag ataaaccaac tttacagtat ataatagaag aagctataga atcaggaatt
     7021 gaagaaattc ttataataac aggtagaaat aaaaaatcta tagaagatca ttttgataaa
     7081 tcagttgaat tagagttaga actagagcaa aaaggtaaga cagagatgtt agaaatggta
     7141 agagatatat caaatatggt aaacattcat tatataagac aaaaagagcc aaaaggtctt
     7201 ggacatgcaa ttcactgtgc aaaaagtttt ataggtgatg aaccttttgc tgtattatta
     7261 ggtgatgata tagtagattc agaggttcca tgcttaaaac aattaataga tacttataat
     7321 gagtataaaa caactgtatt aggtgttcaa aagatagcta aagaagatac taataaatat
     7381 ggtatattgg atgtaaaaca tatagaagat agggtatata aagttaaaga tatggttgaa
     7441 aaacctgcaa ttgaagaagc tccatctgat atagctatac ttggaagata tataataact
     7501 cctgcaatat ttaacatatt agagaaacaa acacctggta aaggtggaga aatacaactt
     7561 acagatgcac ttcaaactct tggaaaacaa gaggctattt atgcttataa ctttgaaggt
     7621 agaagatatg atgtgggaga taagattggt tttttagagg caacaataga ctttgctcta
     7681 aagagagaaa atttaaaaga tgatttaatg gactatatga gaaaaaaagt aagtgaaaaa
     7741 taaatattag aaaagaggta attacaatgg cagttttagt agctggagga gcagggtata
     7801 taggaagcca tacagcaata gaacttttag aatcaggtta tgaagttgtt atagttgata
     7861 acttaagtaa tagtaactta atagttgttg atagaatcaa ggaattatca aaaaaaccag
     7921 taaaatttta taatattgat atcagaaata aagatgaaat gcatattgta tttaaagaaa
     7981 ataatattga atcaattata cactttgcag ctttaaaagc agttggcgaa tcagtagaaa
     8041 aaccaattga atattacagc aataatctaa taagtacctt aaatttattt gaattaatga
     8101 gagaatatgg tgttaaaaag tttgtattta gttcttcagc tactgtatat ggagacccac
     8161 acacttgtcc tatattagag gattttccac tttctgtaac caatccatat ggaagaacta
     8221 aacttatgat agagcagatg ttggtagata tatcaaaagc agataagtca ttggatatag
     8281 ctcttttaag atactttaat ccagtgggtg ctcataaaag tggtagaata ggtgaagaac
     8341 caaatggagt tccaagtaat ctaatgcctt atataacaaa aatagcagta ggtaaattga
     8401 aggaattaag tgtttatggt aatgactatc caacacatga tggaactgga gttagagact
     8461 atatacatgt attagattta gctactggtc atgtaaaagc attacaaaaa ttagaagaaa
     8521 atccaggact tgttgtttat aatttaggaa ctggaaaggg atatagtgtt cttgatttag
     8581 taaaagcatt ttctaaagca agtggaaaag aaataccata taagattgta ggaagaaggg
     8641 ctggagatgt agccatgtgt tatgcagatt cttcaaaggc agaaaaagag cttggatgga
     8701 aagctaagta tgaacttgaa gaaatgtgtg aagattcatg gagatggcaa agcatgaatc
     8761 ctaatgggta tgaggaatag aatgtatgct tatatgtata aatgtaacaa atttgtaaat
     8821 ataaggtatt ggtaatttta tgttataatt agacacatgt agtaatgttt ctaaaagagg
     8881 gggaagttgt agtgaaaaaa agtctaaaac agtatttagt actagcactg actttagttt
     8941 tagtatttgc atgtggcgta ccagaatcaa gtgcagccag taaacatgtg attatagtaa
     9001 attcaagaaa gaacactcta ggatattttg taaataataa acttgtaaaa gaatttagag
     9061 ttgcaacagg taaaaaaggg tcagaaacac caacagggaa aacaaaagta gttaacaaga
     9121 taaaaaacag accatattat aaaggtaata tacctggagg aagtccaaga aatccattag
     9181 gagatagatg gatgggatta gcattaaaag gaacttatgg agatacatat ggaatacatg
     9241 gaaataataa tgagagttct ataggaaagc atatttctgg tggatgtata agaatgcaca
     9301 ataaagatgt tagatggtta tttgaccaag ttcctgtagg ttcagatgta attatagatt
     9361 atagtaatga cagttatgta aagatagcag caaaatataa gataaattta aaccaaacag
     9421 gatggaaaac agaaaacggt aaaaagtatt atgtaaaatc agatggaaca taccaaaaga
     9481 atagttggtt aaaagtaaat gggaaaatgt attactttga tgcttcagga gtaatgcaaa
     9541 caggatggaa aacaattaat aacaaaaaat attatttagg aactgacgga gcaagagtat
     9601 ctggatggaa aataatagat ggaaagactt attactttaa ttcagatgga gtaatgcaaa
     9661 caggatggca agaaaagaat ggaaagaaat actatctagg agctgatggt ttatcagtaa
     9721 caggatggca agaaatagat ggtaataagt attactttga caaaacagga ataatgcaga
     9781 taggatggca acaaatagaa ggtaagtctt actatttaga caaagatgga aagatgctaa
     9841 ctggtgctca aaaaatagat ggaaaagatt atacttttaa tgaagatgga actataaatc
     9901 ctacatggga tacaattata ggtgcaaata gatttgatac agctaagaag ataagttcag
     9961 ttggaaactg gaatgctgat agttcagata ctgtaatatt agtaaatggt aatgcaatag
    10021 ctgatggtat aacagcaact cctttggcaa gtagctatga ctcaacaata ctacttacta
    10081 atacagataa tttaccaaat gaaactgttg aagaaatgaa gttattagca cctaaaacag
    10141 taattttgat aggtggagaa aatgcaataa gttctaaatt agagcaagaa ataaaaacta
    10201 ttttcaatgc agaaacaaag agaatagcag gtcaagatag atatcaaact gctacaagaa
    10261 tagctgaaga acttggaagt cgagaagaaa taaaaactgc atatatagtt tctggaaatg
    10321 gggaagcaga tgctttatct gtagcttcta aagctggaga agaaaaacaa cctataatat
    10381 tagtcaataa agatggaatt acagaagaat cttataaatg gctaacagaa agaaaattag
    10441 aaaatgcata ctttataggt gggccaagtg caattagcga ttcagtaata gccagaatga
    10501 atgatataac tactgaagat atttcaggaa atagaatata tggagatagt agagtagata
    10561 ctaatgcaaa agtaatagaa aaattctatg gagatgctga tttacaggct gtattagtta
    10621 gtaaatcaga tgctttagta gatgctttat cagctggacc attagctgta aaattacatt
    10681 caccaatagt tttaatgtat aattcaggat tatcgtcaga gcaacaaaga gtatttgcaa
    10741 ataaaaaagt tgaaactcct tatcaaatag gaggcggagt atcttatata gtaatggata
    10801 aattaatgga tattcttgga aaatagtgaa aatatatact gaaaaataat tgtaattgag
    10861 atgtgaaatt caatcaataa aagcttgatt gttttacatc tcaatttttt agtataaaat
    10921 taagtttaag ttatataaaa atcttggaat atttttaaaa ctcatataaa atgaatatag
    10981 atggtatcat catatgttaa gagggtgagt aagattataa agaaaaaata tataatgttt
    11041 attgatgaaa gtggaagagc tgatataaat catgaagacc catttacatt gacaggagta
    11101 atttttgaat ataaatatgc agtaagtcaa ggcgattatg tatgttcttt gaggaaagaa
    11161 atggacgcat ttaaagaata ttgttttgga actagcaaca taggaataca tttgacagat
    11221 atatcaagaa aatcaagagc ctttgagaag tttgatgatt cacagattaa gttattttat
    11281 gatgagttgc ctttatttct atctagacta gaatgtacta taatatcagt aacagtagat
    11341 aaagctagat taaaaaaata ttatgcacct tcaaaggaac cgtatgttgt ggcttttata
    11401 catgttcttc agaattttta ttcgtttgta aataatgaaa ctgtagaatc tgcacgaata
    11461 gttatagagt caagggatga tgtctcaaac ttaaaagtac aaaaggcgtt ttttgatgta
    11521 tttaataatg gaacaatata cttagatata gatgaagaat taagagataa ggttaaaggt
    11581 tttataattg ccaaaaaaga agatgcagac tatagagaag gtttagaaat agcagatatt
    11641 ttatgtaatc ctcttagcag agcaagaaga gggctaatag aagctaatcc aaagtgtatg
    11701 caatatggag aaaaaaataa aatattcaaa tctgtcagaa ataaaatata taccccaacg
    11761 agtgtgaatg atataagaaa ttggggtttt aaagaagttc ctattataga gcctgttgga
    11821 ccatggcttg aaaaaatgcg ttagatacaa ttgacaagtt ttaaaaaatc tgatataata
    11881 aaaataacat agttattcag gaactgaatc cagttgctgt tgtataacga taattactga
    11941 gactcggata ccgactgtca gttaggaatc tatactttta aaagtataga tttttttatt
    12001 ttataaaaag aataaaagag gatatttaaa aataacctct ttattaataa attatagttg
    12061 cgcgttttta atgacaaagc ttttaaagtt tttaattgca ggagggaaat atctatcttt
    12121 tataaatgcc aagtaaaagt ttctttccca acttgggtga ataatctgaa ttacttttac
    12181 ttttgtaaag ctaagcatat tcatatttgg gactacagca attccgaaat tttttgcaac
    12241 taatccagca attacttggt cttcttccac ttcatagact atgttaggct gttgatttat
    12301 ttttttaaac atatcatcaa ttatatatct aagaccactt ctctgattaa atactatttg
    12361 aggataaggt atggtttctt ttaaatctat agtatccttt gcagctagtg gatgactata
    12421 tggtacaatt agtactaagt cttgtttggc aacaggtata aattcaattc ccttttcttt
    12481 ttcaagtttc gaacaaaatg caatatcata ttttctttct ttaagacctt gaataatgtc
    12541 agtagttaca ccagtatgaa atttaaaatc tattgattta gtaggattag attttaaaaa
    12601 tttatgcact atatctggga taaagtcagt accaagtgtt ctaagaaaag ctaagtctat
    12661 ttgtccttca ccagtaccgg ttatttttaa tgattttaca cttgaatcta aaatttctag
    12721 cgatttttcc acatctttta aaaatatttt tccatactta gtaaggacta tatttctacc
    12781 ttccttttca aataaagcta ctccaagttc attttctagt aaagaaatag catgacttaa
    12841 gcttggttgt gttatagata aattttctgc tgcttttgta tagtgttcta aatgtgctaa
    12901 tgtaacaaaa tagcgtaagt gatataaatt caatttttgt tcatcctttc atagatattt
    12961 ttctttatat atttgtagaa gttgaacttt aagatatttt ttacattttt attatatatg
    13021 attatgtagt aagaataaaa gcttttaaaa aaatttatcg ttttttgtct gaattataag
    13081 ataaagatat ttatacgaca tagtagaaaa atacaagaat tatcttataa ataaaatcta
    13141 taaacaaaat ctatgaatta atagtgaatt atcaatttgt tattagtaaa gtctcatgat
    13201 acaataaatt tataaaatct agataaaaaa aatcaaaaaa tagttttgtt ttttaagtaa
    13261 aataatacta ctagtagaat aaataaaaat ataaaatcaa aaatactatt gtttataaag
    13321 aaaataaagg gggcaaattt aaattatgaa taatcaaaaa aatctcaata ttccaattac
    13381 aattagttct tatacattag ggacagaagt ttcattccgt gatagagttc gtattgcaaa
    13441 agaagctgga tttgatggta ttggattacg tgctgaaaat tatattgatg caaaaaatgc
    13501 aggtgttaca gatgaagaga tgattgcaat tcttaatgaa tatgatatga aagttacaga
    13561 agtagaatat ataactcaat ggggaacaga agaagataga acggaagctc aaagagaaaa
    13621 agagaaaaat gtttatcata tggctcactt atttaatgtt aaacatataa attgtggatt
    13681 attagagatg ataccagaag aacaaattat aactgcattg ggagaattat gtgacagagc
    13741 aggtgaactt attataggtc ttgagtttat gccatatagt ggtgttccag atttggctac
    13801 tgcatggcgt gttgtgaaag gatgtaatcg tgaaaatgcg atgcttatat tggatacttg
    13861 gcactgggct agagcaaagc aaacagcaga cgaccttgca cctattccag cagataagat
    13921 agtatcaatt caagtgtgtg atgttttaga aaatccttat gaaaaactta gggatgaatc
    13981 actgcatgac cgtttggctc caggtgaagg atttggaaat acggaggaat ttgtgaaaat
    14041 aataaaagaa cacaatatat taccaagagt cataggggca gaagtaatat ctgattctct
    14101 aatatctaaa ggattatcta ctgctgctga aactgtattt aatgcaacta aaaaagtttt
    14161 ggataaagca tggtctgaag tatctccaaa ataaacttat acaatataaa aatatacgaa
    14221 gtattataat ataaaacttt taaatataaa acttgaaagg aaggaaaatc ttatgaagat
    14281 tagttctatg tttacaccta taagaattgg ttcaatgaca gttccaaatc gttttgtagt
    14341 tcctcctatg ggaaacaatt ttgcaaacac agatggtact ttaagtgaaa cttctaaggc
    14401 ttattatatt gaacgtgctt taggtggctt tggattaata actatagaat ctagtgttgt
    14461 agacaaaaaa gcaaaaggag gtcctcgtaa gccttgttta tatgatgata gcacaataga
    14521 tagttttaaa aatgtaatag atgcttgtca tgatgcaggt tcgaaggtat ctatacaatt
    14581 acaacacgct ggttctgaag gaaatgaaaa aatagcaggt catccattaa aagctgcttc
    14641 tgcaatacct gcatcaaacg gccgtaatac accattagca ataacaacag aagaaatata
    14701 tgaacttata gaatcttatg gagatgctgc attacgtgca caaaaagctg gtgcagatgc
    14761 tgttgaaatt cattgtgctc atggatatct agttagtagt tttatatcac aaaggacaaa
    14821 taagcgtgta gatgagtttg gtggatgttt tgaaaataga atgagattac ctcgtcttat
    14881 cattgaaaat atacgtaaaa aagtaggcaa ttctctagca atattatgtc gtataaatag
    14941 tactgatgat gtactaggag gaattgatgt acatgatagc tcagtaatag cagcatatct
    15001 agaagattgt ggaatagatg gacttcatgt ttcgcgtagt atacatatac atgatgaata
    15061 tatgtgggct cctacgactt tacatgctgg attcagtgca gaattagtta ctgaaataaa
    15121 aaaagctgtt agtataccgg taataacggt tggacgttat acagagccac agtttgctga
    15181 attaatggtt agacaaggaa gatgtgattt agtagcattt ggtagacaaa gcttagctga
    15241 cccagaaatg cccaataaag ctaaaaatgg aaagctagac gaaatgatac catgtattgc
    15301 atgcttacaa ggatgtgtac caaatatgtt ccaaggtaaa cccatatctt gtcttgcaaa
    15361 tcctatatta ggacatgaag cagagtttaa gtcagccgaa attagtaaag aagttcttgt
    15421 tgttggtgga ggcgttggag gtatgcttgc atcatggata tgtgctaaaa gaggtcacaa
    15481 tgtaacttta gttgaaaaat ctgaagttct tggtgggcaa atgcgtttag ctgcatatcc
    15541 cccaggaaaa ggagatatta ctaacttagt tagaagttat atttcaaaat gtaatcaata
    15601 tggtgtaaaa atatgtacta acacagaagc tacagtagaa cttataaaag agaaatcacc
    15661 tgatgcagtt attatagcta ctggggcaac tccacttgta cttcctatac caggaatcaa
    15721 tgattcagga ttaatacatg ctgtagacct tcttgatggt aaaaaatctt gtggtaaaaa
    15781 agtacttgtt gttggtggtg gaatggttgg atgtgaagtg gcagctttcc ttggagaaca
    15841 agaacatgat gttactgtta ttgagcttag agaagaagtg ggtgcagacg taatttcaga
    15901 acaccgtaaa ttccttatga atgattttga aaattataat atacatactg ttactagtgc
    15961 aaaagttgct aaattttatg atgatggtgt tgactattta cttgcagatg gtacagagca
    16021 tgatttaaaa ggttttgact cagtagtact tgcaatgggt tctcgtaatt acgacccatt
    16081 aagtgaagtt ataaaagaac ttgtaaaaga aacttatgtt gttggtgatg cagtaaaagc
    16141 tcgtagagca ttagatgcaa caaaagaagc ttttgaagta gcattaaata tataaataat
    16201 aaataaattt ttctttgtaa ataactgaat aggttatttg caaagaaaat gataatattg
    16261 ggaggaaatt agagttatgg aaatttcagg tagaacagga ttatttgcat taataggtac
    16321 accagttggt cactctaaat caccagtgat gtataattat agttttgaaa aattagattt
    16381 agattataga tatttagctt ttgatattac agtagacaag gtaaaagagg cactttcagc
    16441 aataaaaact tttaatataa aaggagctaa tgttactatg ccatgtaaat ctgctgttac
    16501 agaatatatg gatgaacttt ctccagcagc tcgtataatt ggagcatgta atacaattgt
    16561 aaatgacaat gggaagttag ttggtcatat aacagatggg gtaggctatg ttcgtaattt
    16621 aaaagaaaac gaggtagaag ttaaaggtaa aaaggttaca attatgggtg ctggtggtgc
    16681 agcgactgct atacaagtac aatgtgcatt agatggagca agagaaatat ctatcttcaa
    16741 tgctaaggat gatttttata aaagagcaga gcaaacagtt gagaatataa aaaaagaagt
    16801 accaaaatgt attgttaatc tatatgattt agaagataca aacaaacttt atgaggagat
    16861 agaatcaagt gatatattga caaatgcaac tcttattggt atgaaacctt atgacaatga
    16921 aacaaatata aaagatacta gtgttttgag aaaggattta gtagtaacag atgttgttta
    16981 taatcctaga aaaacaaaaa tgattgaaga tgctgaatct aatggatgta aggctattgg
    17041 tgggttagga atgttattat accaaggagc agaatctttt aatctatata ctggattaga
    17101 aatgcctgta aaagaggtta atgaattgtg ttttaaatag agataaaagt gattgcttaa
    17161 acaatggaaa aacatatgta tttcaatgtt tttagtaata aatttcaaat tgtcatagtg
    17221 ggttttgcat tatagtctag ttaaatttga agttttaact gactatgctt taaacccatt
    17281 atggggataa gtttattgta cacaaaaatg ttaataaatt aacaaggata ggagaagaac
    17341 tatgaacaat aaaaaatatt atcctactgc catagcgttg tatttttcat attttttact
    17401 tggtattgga atttcaattt taggacaata caaaccagaa ttttcatcaa tgtggggggc
    17461 taaaacacta tcagatggaa cattggatgt aagtattgtt ttagctgtta ttgctgcact
    17521 aggattaggg agacttatat cgtatccatt tgcaggacct atttcagata agtatggaag
    17581 aaaagtaagt ggtcttatag gaaactttct acatgcaatt ttctttgttg gaattgtatt
    17641 ttcaccaaat ttttatatag catatatatt tgcaataatt ggaggggcag caaattcatt
    17701 cttagatacc tgtgtaacgc cttcttgtat ggagatattt gctagtttag gaacaatagc
    17761 aaatatgttt actaaattta caatagcttt agcacagttt ttgcttcctt ttataattgg
    17821 atttgttgca gcaaattcaa tatcatttaa agttattttt atagtaacag cgattcttat
    17881 tgtagtagat gccattttga tagctgtgtt accatttcca ccagctaata atgttattga
    17941 taataaaggc aaaacagtta aatcagagaa gatgaaattt acaccaacaa gtatagctct
    18001 tgtttgtatt ggatttacat gtacatctac atttgtatta tggttaaatt gtaatcagga
    18061 attgggaaaa ttatatggga tggctgaccc tacaaaaatt caatctttct actcaatggg
    18121 agtaatatgt gcagtattaa taacttcttt acttattaaa aaatatatta aaccaataag
    18181 aatattagtt atatatccta taatagcttt acttatgcta ttagttgtat attttgttca
    18241 aactccaact atttgtatgc tagggggatt tgtaataggt tattcagcag caggaggtgt
    18301 tcttcaatta acaacatcaa ctgcaaatga aatgtttcca actaataaag gaaaaataac
    18361 gtctatagtt atgattgcat ctagtatagc aaactatgtt attttaaata tagcaggtat
    18421 tatcacaaaa tcaggtggag ttaatggacc aaaatatgtt gtattattta atgtggcaat
    18481 aacatttgtt ggaattttac ttgcattgtt tgtaaacatg agatatgaaa aagaaaaagt
    18541 gtatgattat gatgtttaat attagtataa tatatttata gagaaatcca aatttaggat
    18601 gggtttctat taaaaataat taaaattaat aaggatatta tttatagtta taagataggg
    18661 tatttacata attaataata aggatattac tagaatttca atttctagta atattttttt
    18721 gcgtataaat gtaaacttgg cgaaataagt agtataatca atataaatta catcatgtaa
    18781 aggaggataa tttggtggag aatgttttta agcgattaca ggaatttgat ggttatgatg
    18841 gatacaaaga atcatttgaa atgaattacc tatgtatata tgaaaatata cctttaagag
    18901 aacaagtaga gcttgcaaat aatctaatag atggaatttt aaatatgtat aaaagtgaat
    18961 ctaatgaaat ttatttattg gaggatagta attctaagtc tttaatttgt tattttgaaa
    19021 tttttatgaa aaagataaat actttggtaa aagaaatgat tattgatgag aaatggttat
    19081 ataaattaac taaagaatta atatataaga gtaaaaaagt tgagtatgta aaactaggat
    19141 tagttttaag tgagaaatat ctagatgtag aaaacttaag agaagttgta gatacatttt
    19201 ctaaatctgg agaatacgta ttttatttga gtaatgctat taaaaaaatt gaattttaca
    19261 atacatattt atttaattta tctaaaaaag ctacaggttc aataaaagta tttgctattg
    19321 ttaatatgga aaatttagat tctaaaataa attcctattt aattgaatat ggttataaag
    19381 atacgaagta ccaacgatta ttgatgaatt atattatttc aattgtagac ttgaatgagt
    19441 atcttgaaaa acgagattta gatagagaaa agataaacaa tttatcactt cttatatgta
    19501 actacttgct aagtgtagaa tttaaataca taggaaataa attagaatta gtgaatcgat
    19561 ttttacctat tgtagtaaat tatgggacta attttgaaag tttatattcg atttttctaa
    19621 tagctataaa cgttttaaaa gatgaaaata tagaatgtaa taaggttgaa tttgaaaaag
    19681 aaataaatga tatcttgctt agtgaaaaat ggaaaagtat atattttgag gcattaaaag
    19741 atgctagtgg aaaaacagaa gatatgataa aaatgtcaga aatttataat gtaaacttat
    19801 cttttgatga cttattacca tatttaaata gagacattag agattttgag gtatattggc
    19861 atatatctaa aaaaggaacg acaagttcta gattaaaatt attggatttt tttgaaaaga
    19921 catttaaagt agatgattta attggaaaaa tgaaagatat agaaaaagat aaattgactc
    19981 aagaatatta tgatgatatg ttatttttta ttgtgcttaa aggaagtaaa tcactatatc
    20041 ctgaaggaaa gaatatatct ttaaaaggaa tatttggaaa tataaatgaa gtaagaaaag
    20101 aatctataaa tattttaaaa agatatagag agaaattatc tcttgaagaa ttaaaagttg
    20161 ttaaggaagc atatgaaaaa gaaaaaaata tcatattgaa ggatgaatta agaagagtat
    20221 tatatgaaag taacaactta aaaaaagaat ttgtaaatat tgaaaaaata aaagtagatg
    20281 aacatggtaa ggatatctat ttaacttcaa taactgttgc tggttcaagg tttagaaata
    20341 gagaatatct tgaaaaagaa ttggagaaga gtaagatata ctatctaatt agagagaaag
    20401 ataatctata tgatgaaaaa gctattaaaa tagttggtga aactggatat gtaataggct
    20461 atgttccaag aaaagaaaat tatattttga gcaatttatt agatggagga aaactattgt
    20521 attgtagagt aactgaatat aatttatatg aagattgtat ttacgcaaat gtgtacttga
    20581 gctataaaga tgtaattgaa acggttgaaa actctttaaa gatggtttta gataagagta
    20641 gaataaaact tataaactaa gacgtctata tactgcttaa atgatgtaat cctgattagt
    20701 taattatttt ttctggaatt atggtgtatt ttatgagcaa tggtaatgaa taggaaataa
    20761 aaaaacaatt gttttgtata tatgatgtgt ataaatatat ttatatattc atatttacaa
    20821 aataattgaa atttatgtta ttttatgatt ttaaatttag atggaatttt aacgtctgat
    20881 ttatctttta ttttagaagc ttgttcctta actttattta ataattcaga ttcatctaca
    20941 actaatactt ttctttcctt catcaaccat ttaccatcac aaatagtaga atctacattt
    21001 tcactagtca tagaataaac tatatttcca attggattat gcattggtaa acaatggatt
    21061 gtatctggat ttaaaataat catatcggct ttttttccta cttcaagact accaatttct
    21121 ttttctaata aagcacattt tgctccattt atagtagcca tttctaagac ttcttcagct
    21181 ggcactactg ttgggtttaa agttctacct ttatgtatta atgatgtaag atacatatcc
    21241 ctcataaggt ccattctatt atttgaagga gctccatcag taccaattga aacagatatt
    21301 cctttttcta acatttcagg aattgatgca aatcctagaa caactttcat agctgaacct
    21361 gggtcatgag aaacttttac atcatgtaat ctaaataagt caacttcctt atgagtaagc
    21421 caaactgtgt gagcggctaa aagatttggt cctaatacac ccagtttatc aagatattca
    21481 actgttccaa ctccattgtt ttgttttaca aatgaaattt catcagctat ttcagcaata
    21541 tgcatatgta tacctgtatt gtatttatca gctaactctt tagttctaac aagtaattct
    21601 tcagttgtat taaaaatagt tcttaatgca aaccatattt taattctctc attagatgtg
    21661 ttattaaact tgtggaataa gtctaattga gtgttgattt cctcatcagt agttttttgc
    21721 catgcctcag gaagaccatc tcctgtatcc ataactgatt tagctaaaca agctctaagt
    21781 ccagttttat caacagcttc aaccatagca tctacatatt gaccaccaga ttctaaaaat
    21841 gtagtgacac cagactttat caattcaaca caacatgcag tagatgaaat caatgaatct
    21901 tcataattga agctactttc gtatggccaa attctttctc taagccaagt taaaagtaca
    21961 acatcatctg caagaccacg tcctaattgt tgagataagt gaacgtgtgt attgactaac
    22021 cctggcatta gtatttttcc tttagcatca tatatctctg catctttatc tacaatacta
    22081 atatcaaact taccaacctc ttttataata ttattttcaa taagtatgct tccgttttca
    22141 tatacctctc tatctgcatt catagtaatt agatatgcat tttttataag taattgtgac
    22201 ataaacacca ctccttttct atgtattttt atatctatgt attatgataa taaattttct
    22261 atacaaagtt aatagcaaaa tcaatttaca tataaagtga ttttgctatt tataaattta
    22321 aaataaataa gattttattc tatataattt aaaatattga cctagaacta tttaaaatat
    22381 taacttagaa ctaagtttac aactgcaagt aaacctataa tccaaagtgt aatactgatt
    22441 ttatcagttt ctttagctac cacatgcatt attatatatg aaataaatcc aaagcataaa
    22501 ccaatactta tactataagt taaaggcatc aataagactg taacaaaagc aggtactgct
    22561 attttactat cagcaaaatc aatctgtttt atgctttcaa acatgaacac accaaccatt
    22621 acaagtgcag gagctgcagc gtaagtagga actataccaa gtaatggtgt aaatataagt
    22681 gtagctaaga ataatgctgc tgttactatt gaagcaagtc ctgttcttgc acctgctgca
    22741 ataccagaag cagactctcc aaatgttgta acagtactag ttcctaagaa tgagcctatt
    22801 aaagtagaag aaacatctgc atacaacatt cttcctaaat cttttatttc accattctca
    22861 tcttcaagac ccatttgttt acaacaagta ataagaaatg ctaatgaatc aaacatgtct
    22921 ataaacatga aagagaatat aggtccaatt aatgagaatt tcaatgcacc tacaatatct
    22981 aatttaaaag caactggtgc tatactagga ggtgttgaga ttattgctgt tggcaactcc
    23041 acaaatccaa atataacaga tattattgtt gcaactacta tacttatcaa tattccacct
    23101 ttaacttttt tatattctag tattgccatt aagataagag caaaaagagc tattaaaact
    23161 gttggtgtaa aatctgcaag tgaaactata gtatctggat tagctattat aacacccata
    23221 ttttttaaac caacaaaagc aaggaaaaga cctatacctg ctgaagatgc tactgccaat
    23281 tctctaggaa ttgcgtctgc aattgtcttt cgaagacctg ttactgcgag tgtaaagaag
    23341 acacatccag atagaaaaac taccccaaga cctgtttgcc atgggattcc tgcccctata
    23401 accaaagagt atgtgaaaaa tgcatttaat cccataccag gagctagagc aaatggtgca
    23461 tttcctaaaa tcgcaaatat aagtgtacca atggctgtag ctagtatggt aactgttata
    23521 agggcggctt tatccattcc agcctcagat aatattgctg gatttacgaa aactatgtat
    23581 gccattgtta agaatgtagt aactccacct attacctctg ttttaaggct agagccacgt
    23641 tcgctgaatt taaacatttt gtctaaaaag cttaatgatg aattatttcc acctggttgt
    23701 acattgttag tgtttggtac catttttacc tccaaaaaac tttaagatat aaacaaaaaa
    23761 atatatattc atatagatag aatagaacat atattcaagg ttgacattct attcatagaa
    23821 aacccattta aggtgagttt gtagaaacta ttagaccgta ttactaatat tatatgaaca
    23881 ttttctattt gtttttctgt tgtattatat cgtaatattg tattatttac aataaatata
    23941 ataaaattat tcgttttttt gtcgatttta taaaaaatta aggttattta ttggcaaaaa
    24001 ttactgtttt aaataattga atatgtatga gtataatgtc aattattcta acttgtttta
    24061 tactattaat ggaagtttat tataaatgag tttgtgatat aattataaaa gtactgaata
    24121 cacggatatt tcgtaaaaac tttttaatat attaaatatg tatattaagt taatatatta
    24181 aaaaaaataa tatcaaaaat aatataattt atagtggagg ggaaaatgac taaaattatt
    24241 aaagtacaaa aacctaaatt tacaggagaa ttagaaatta ttgaaaatct tgaagatata
    24301 cttgaagata tatttaatga tgaaaaaata tttaataaaa ttatagaaaa tgaagttata
    24361 agtgatttag attctgtaag agttagcttt gaatcttgta tatttagaaa tgtatccttt
    24421 gaaaattgca atttaaaaaa gattgattta cttgatgttg tatttgaaaa ctgcaattta
    24481 tcaaatacat tttttgatgg aggaagtata tatagagttg aatttaaaaa tagtaaattg
    24541 ataggtgtta gatttgatga ttgtatactt aaaaacgttt tgttcaagga tgttttagct
    24601 aagtattcta acttttcgtt ctctaaattt aagatagttt ctattttaga ttcaaatttt
    24661 gaaaattctg tatttcaaga agtaaaaggt gacaatctag tatttgagta tacaaactta
    24721 aaaaaagcaa actttaatgg aactaaactt tctggcatag attttactac taataatatt
    24781 gagggaattg agattggtat agatgatatt agaggagcaa tttttgatgt gtcacaagca
    24841 attgatttaa cgaaattgat gggaattatc ataaaatgaa aaaaatattc ataagataaa
    24901 ttgggaaaac gttaacaatg tgtgaatttt taccagattg acatttttct gaaaataaag
    24961 taagattatc ttaatattca aaatattttc aaagggggta atttagtgtg agtaagcaga
    25021 tgagagatat ttttatagtt ggttttgctt tattttctat gttctttggt gcaggaaatc
    25081 ttatgtttcc tccattttta gggatggaat ccggcaaaga ctggttaatt cctcttattg
    25141 gatttgttgt tgcagatgct ggtatagcaa tgcttgttat tatagcaaca gcaagatgta
    25201 agggaagcat ggatgatgta cttaggagag ctggtaaagg gttagcaaga gtaatgagtg
    25261 tagctgctct agcatgttta gcacttcttg taataccaag aacttgcgca acaacatatg
    25321 aaatgggtat tatgcctata tttggagacc atggaccagt agctaaagct atattttcaa
    25381 taattttctt tgggttaact ttagcattta ctattaggtc ttctaaggtt atagacataa
    25441 taggtaagta tttaactcca gcgttattat tagtattatt tgttttaata gtaaagggta
    25501 taatttctcc agcaggacct atgagtcctg aacatatgat agataaaaat ctatttggtg
    25561 aaggtatatc acaaggttat caaacaatgg acgctctagg tgcagcatct atggcaacta
    25621 taatgttgat gtctataata gctaaaggat atacatcaga taaagaccaa ataggaatga
    25681 ctattaaagc tggttttgta gcctgtgtat ttctagcagt agtatatggt ggtcttactt
    25741 atttaggtgc aacagtttca actttatatg acactagtat acctcaagca tctttattag
    25801 ttgaaataac tagtatattg ttatatggtc aagtaggaaa ggtaatttta ggtgtaattg
    25861 tggcacttgc ctgtctgact acttctacag gtttaacagc atcaatatca acttattttg
    25921 aaggaataag taaggcgagt tataaacaac tggttatagg tatatgtgta gctagtatgc
    25981 ttatatcaaa tttaggtgtt gattctataa tagccatatc agttccaatc ctacaaacta
    26041 tatatcctgt tttattagca atagttgtta tggagttggc tggtaaacat ataaagaatg
    26101 ataatgcttt taaaggtgca gcatatgtta cattagtgat tagtttatta tcagctataa
    26161 atggaatgac aggagctgtt ccatttatac aaagcttacc acttgcaggt ttgggcttta
    26221 actgggtaat accagctata ttgggtggaa tagttggtaa ctttataaaa agcaacaaac
    26281 aagtagcata ataatttgta attaatttaa ttttataata tattaatttt tagtactatg
    26341 ggtaggttta atttaaacct aatctaccca tagtactttt tagattggat tagaatagca
    26401 tgtatataat tttttataat ataatttttc tttgaagtat gataaaatta gtaatagtga
    26461 taagaaagta caaatttagg aggttttaat taatgcttag aaaaataaca gcatttatgc
    26521 tatctatatg tttaattatt ggacttacag gttgtagttt acttgtgggc aagaaagata
    26581 aagagaaagt tatagatgat tttaaagttg cagtggtaac tcaaccatta tctgaaaata
    26641 aagtacaata caatatggtg gaagagatgg caaaagaata tgaggaagag aataagattg
    26701 ataaagacaa agatggtcaa actaaagtta aacaaactat aaagcatgtt gtacttccag
    26761 aaaattttac ttctaatata gatagtgcta ttaataagat agtaaagttg gctgacgata
    26821 aagaagttca ggctattgta gtttcaactg accaagcggg gcttttacct gccttacaaa
    26881 aggtaaagga aaagcgtcca gaaataataa ctatttctgc ccctatggga gatgataaaa
    26941 atcaattaag ccaatttgtt gatgtgaatc taggggttag tgcagaggaa agaggaaaag
    27001 tattagctga acgttcaaaa gaaatgggag caaaagcttt tattcattat gcctctacag
    27061 atgatttgaa agatgttaat atagccaaaa gattagaaat gattaaagag acttgcaaaa
    27121 atataggatt gccatttgta caagttaata ctccaaatat aaatgctgaa gaagacaaaa
    27181 acaaagttaa gcagttttta aatgaagata ttgaaaaaca agttaaaaaa tatggaaaag
    27241 atataaatgt atttggtgta aatgattata tggatgaagt tatattgact aaggctttag
    27301 aattaaagta tatagttgca gagcaaagta atccatcacc aatacaaaca tatcctagtg
    27361 ttatgggtct gaaaatatct aaaaaagatg ctcaaaatta tgataaaata aacgacatga
    27421 taagtgaaaa ggctaaggct tttggtatgt caaatagact tggtggatat cctatgccaa
    27481 tggatgcttt tctaccatca ttagcaattt atcttgccac tgaaatggta aaacaagatt
    27541 taacacaaga agatgtatgt gacccagact atttagaggc gtttacagaa ttaagatttg
    27601 ggataggttc agaatttaca cctctaacag aggtacttta taattatcaa tcagtaattt
    27661 taagccaatt aatttattaa tattaaggaa gtgtctcaaa atgaactttt tagtttatga
    27721 ggcactcttt ttgtattaaa taaaatatat ttttatcttt ttaacaatta aaaagaagct
    27781 tatctctatg ttgagatagc ctcgttttta acataaataa gttttattat tttataaaaa
    27841 atttatagaa aaataaaaaa ttataattat atataaattt tgataaagta aagatgaatt
    27901 actataggtt taaattatat tattttttat tatttttatg cctttctcag ttattgatga
    27961 gccttttctt cctttactag aaattaagta tccattgcgt gtcaaatatt ctagcatatt
    28021 tcttacttcg tattctgtga cattatattc cataaatttc attaactttg tcctccccat
    28081 tggtttcatg tgcaatgatt ttaggataga aatgcatatg tttttgtcat attggcttaa
    28141 atcattgaat atggacatat tacttgacat atgattatta tttaattttg aagatatgtt
    28201 ttgaggtaaa tcatttattg taagataatt agagcccatg taagccatat attcaacaca
    28261 atttctcagt tctctaatat ttcccatcca agtgtatgaa tttaaagctt ctaaaagact
    28321 cttatcgagt tttatgtttt tatagggaag ttctttctaa gtaaagactt atttacaaaa
    28381 gtaactttgt taaaagtatt atagctgcaa actttagaga cgatggaggt actggttcta
    28441 gagaaggatg gttatatgca ttgacactaa taccatcagt tatgtttgca cttggagtag
    28501 ttagagttat agaccactta gatggaatga aggcagctca aaagcttctt agtccactat
    28561 taaagccatt attagggtta cctggatttg cagggctaac cttgatagct agtttacagt
    28621 ctacagatgc agctgcatct atgactaaag aacttaaaga tgatggatat atagatgaaa
    28681 aacaaaaagc tgtattctgt gcatttcaat tttcaggcgc ctctgcaatt acaaatttct
    28741 ttgcttctgg agcagcacta tttccattta taggggatgt accaatattt ataccactta
    28801 ctttaatatt aattatgaag tttgttgggg caaacttact tagattatat ttaaataaat
    28861 ttgacaaggg ggaagcttaa catgagtgag gtagtaaaaa aagaagtaca agttaaaaaa
    28921 ggaaatgtga tagatgcgtt tattggagga gctagaaatg gattccaaat aagtaccaat
    28981 tcaatggctc caaatgtcat cttcggtttt gctataattt ctgtattcaa tttaacaggt
    29041 cttttagatg taatagggac tatatttaca cctgtaatga gtatatttgg acttccaggt
    29101 gttgtagcaa cagctataat gacaatagtt atatcgcgga gaggattccc attattatac
    29161 acaaaaacta aaatgtaaaa ctacatatat tggattagga gctgatgcta caccagggtt
    29221 tcataatcca aatgtaacat ttgatacgaa agctttagaa tatggtgtag attggttgaa
    29281 aagagattgg gataatatcc aaattaataa ttctacattt aaaattataa ttatagaagt
    29341 tatagaaaaa gattgcttat gttaattgaa aaataattaa tatgggcaat cttattttgc
    29401 tataatgaaa cttattttga aatattgaaa aaatatggta gaaaaagcaa ttatatttta
    29461 agtctattgg gcaaaaaagt gatattataa tattaaggat tataaaatga tatcaaattg
    29521 aatctagtaa tacactaaca attataaaaa ttttattttt aggagggaaa aaatgagtaa
    29581 gtataacttt gacagagtaa tagatagagt aggaacagat tgtgtaaagt gggattttag
    29641 aactaattgc tcaactaaag cacaagaaga tggtttgcct ttttggattg cagatatgga
    29701 ttttgaatgt gcagaaccaa taatagaagc attacgtaaa aaagttgaac ataaaatatt
    29761 tggatatagt tccaatgata gtgataaata ctttaatgca gtttgtgatt ggtataaaag
    29821 gagatttaac tgggaaataa atagaaaaga tattattttt agccctggtg ttgtacctgc
    29881 tgttgctatt ttagtaagaa tattaactaa ttctaatgaa ggcgtgataa ttcaaaagcc
    29941 agtgtattac ccatttgaag ctaaggtaaa gagtaataat agggaagttg taaacaatcc
    30001 tctaatatat gaaaatggga cttatagaat ggattatgat gatttggaag aaaaagctaa
    30061 gtgtagcaac aataaagtac tgatactttg tagccctcac aatcctgttg gaagagtttg
    30121 gagagaagat gaattaaaaa aggttgtaga aatatgtaaa aaatatgacc tatggataat
    30181 agctgatgag atacattctg atttaattag aaaaggattt aaacatactc ctttacagtc
    30241 attatgccca gagtacaagg ataaaatagt tacatgtata gctccaagta aaacatttaa
    30301 tctagctgga atgcagttgt ctaatataat agtgaataat gatgaactaa aaaagaaata
    30361 tcaagaagag gttacagctg ttggagttgc aacttcacca aatccttttg ctattgtagc
    30421 tacaatagca gcatataata aaagtgaaga ctggcttaat gaattaaatg aatacttaga
    30481 taataatata caatttattg atgaatactt aaaagaaaac ctaccaaaag taaaacttgt
    30541 ttatccagaa ggaacatatc tagcatggtt agattttact gcttatggat tgaatgaagt
    30601 agaacttgag gatttaatgt ttaaaaaagc taatgtatta tttgatgaag gttatatatt
    30661 tggaaaagaa ggaataggat ttgaaagaat aaatgtagct tgtccacagt ctttattaaa
    30721 agaatgtatg ggcagattaa agactacatt tgaaaattta taaagtatag gtaaaaagta
    30781 ataaagtgtt taaaaataaa aaaataataa gcttgacaaa ttttaactaa tcacttatca
    30841 tactattatg aataaataga aaacataaga gagctaaatt ttattgaatt aaactcttaa
    30901 agactttgaa aggaagagta gttatagaat gtaattaaag cgagttggtg atggtgtgag
    30961 accaatatga agtctatagt gaagaacatc ctggagttgc taaccgaaat tttaaaagag
    31021 taggcttagt cggaaccaaa ccgttatcat attggggtgt atcgagtaat atctgtacat
    31081 tgtgagctgg gtttgaattt atttcaaact aatcagggtg gtaccgcgga aaagcaacct
    31141 ttcgtccctt ttatataggg atgaagggtt tttttaatgg aaaaatatat aaatataggg
    31201 agtgaatgat atgagcacaa taataccaaa ggattataaa agtagtttga acgtaattga
    31261 tacacaaata gctataaaaa aattaaaaga tttttttgaa atgagactaa gtaatgaatt
    31321 aaatcttata agagtttcat ctccgttatt tgtattgcca gagacgggtg caaatgataa
    31381 tttaaatgga gaaaaggctg taagctttga tataccattt atgaataaaa atgcagaaat
    31441 tgttcaatca cttgcaaaat ggaaacgatt agctcttaaa aaatatgggt ttgaagttgg
    31501 cagtggttta tatacagata tgaacgcaat aagaaaaaat gaagaattag acaatataca
    31561 ttctctttat gtagaccaat gggattggga attggttata gataaaagta gtagaaatga
    31621 aaagactcta aaagatgttg tgaaaagatt gtatggagta tttaaggata ctgaaatttt
    31681 tgtatgtagt atgtatgaag atataaaaga aatattacca gaagaaataa cttttataac
    31741 atcacaagag ttagaagata tgtatccgga attatcacca aaggaaaggg aagataaaat
    31801 cgtaaaagag aagaaagcag tgtttataac tcaaatagga aaaacattaa tatcaggaga
    31861 aaaacatgat ggaagaagcc ctgattatga tgattgggaa ttaaatggag atatattatt
    31921 ttacaatcct gttttagatt cagcactaga attatcttca atgggaatta gagttgatga
    31981 agaatctctt gatagacaat tgaaacttgc tggatgtgaa gagagaaaag agtttgacta
    32041 tcataagatg cttttaaatg gagagcttcc atatacaatt ggtggaggaa ttggtcagtc
    32101 aagaatatgt atgtatttct tacaaaaagc tcatatagga gaagtacaag ttggagtatg
    32161 gccacaggat atgattcaaa attgtagcag tgcaggaatt gaattattat aatttaaata
    32221 taagctaatt aataaaattt atgtctaata tgttcatcaa attaattata tcggttataa
    32281 tgtagaataa cctataatta taagaggtga taaatatgga ttttctaaaa ataattatta
    32341 atatagtttt agatatttta aaaaaaattt tggtgagatt taaaaatgct aaatttggtt
    32401 tggtttttgt atttgattta cttaaactac ctgattttat gacagataaa agaatcaata
    32461 tagctgataa aataaaagta atttctgtat taatttttac tatatcttac tttgtatctg
    32521 gggttgatat aattccagag atgattgctg gggcatttgg ttttatagat gatgtgatag
    32581 ttttaatttg gagtataggt atagtgaatg aagaaatcaa taagtataga gttattgcaa
    32641 aaaaagataa acattcaact ataattgaaa atgtaaattt ttctataaaa gatgaagaag
    32701 aataataatt tatatgtgta tgctaacata ttatataaca tcatatatta ctagatagta
    32761 gctaaatcct agtttttgct actgtctagt gataaaaatt tgactgacct ccaactgaga
    32821 aacaaaaaat atacgattaa ctaacatcca acgaacataa aactgactat tatttaaata
    32881 agattttgct caaattttca gatatttctt ttttcacttc ttctaaaaca tgagagtatt
    32941 tatttatagc tatttttata tctatatgac ctaatctgtc taaaatagtt tttttattag
    33001 ttccatataa taacatcaaa gttacatttg tatgtctaag ctcatgtaat tttatatatt
    33061 taaagatgtt tttaaaagat ttagactaat atcataagta ttcttaatac attgaataac
    33121 tatgatttta ttatatcaaa tctaagttat aaatactatt tgatgataga gtagttgtat
    33181 acagaaatat atagatgaac ttttgaaaaa gtaataaagt agaggattat ataattaaac
    33241 ataatggata agtagaagga gcagaaccaa tagtatagaa ggttcttttt ttatgcgttt
    33301 agatgatttt taagtcttgt tttatgttaa aataataggt aatacagata taacaatagt
    33361 ttttttagca ataaatatat ttataaaatt ggcaatatta agaatagcat tttatgcttt
    33421 gctagaatgc ttaagagcaa taaaaacaca taaacttaat ggttctcttg taatcaatgg
    33481 aggaaataag aaaagtcgca atgctggttt gtatattttt tctagaatta gttgacatac
    33541 ttataaattt taacttttta tttatgttgc tataagattg ggttgatttc ttatgattaa
    33601 ataatctagg aatatcagaa tttttcttta ttttatttat tttgtataaa aatctaagta
    33661 tattaaaaaa tatgtactta aatggattac cagttattaa agattaaaag agaaaatagg
    33721 tagtttatta gataccatgg cagatgaatc aactattaaa ggaggagaat aaataatgaa
    33781 aatatgtata acagtaggac acagtatttt aaaaagtgga gcatgtactt ctgctgatgg
    33841 agtagttaac gagtaccaat ataacaaatc tcttgcacca gtattagcag atacatttag
    33901 aaaagaaggt cataaggtag atgtgataac atgtcctgaa agacaattta gttctaaaaa
    33961 tgaggaaaaa tctcataaaa tacctagagt taatagtgga ggatatgact tacttataga
    34021 gctacattta aatgcaagca atgggcaagg taaaggttca gaagtactat attatagtaa
    34081 taaaggttta gagtatgcga ctagagtatg tgataaacta ggtacagtat ttaaaaatag
    34141 aggtgctaaa ctagataaaa atttatatat cttaaatagc tctaaaccta cagcagtatt
    34201 aattgaaagt ttcttctgtg ataataaaga agattacgag agtgctaaga aacttggata
    34261 tgaaggaatg gctaagttaa ttgtagaagg tatattaaat aaaggtgttg caaataatga
    34321 ggttaagcaa atgtataaac atacaatagt gtacagtggt gatgataaag tgtcagcaga
    34381 gattttagga ttatattata agagggcaaa agaaaattat ttagtttcag atataaaaga
    34441 ctatgagcca catcaaacgc aaaatcttta tgttataggt ggtgagactt gtaataaaat
    34501 gaaagaaatg agcaatacta caggagaaaa atttacagag atttattctg atgatgtatg
    34561 gtcaacaatg gataaggcta tagagtttgt aaaagaaaaa ttataattta aaatttttat
    34621 ttaaacaaga atgtagataa gaaaaattta tatcaaaatt tttcttatct attttttctt
    34681 ataaatatat ttattaaagt tataaaagca tatatttcta gaaaaaatgt gttttttgac
    34741 tatttatgcg tttttgatga ccagttagac aataaggatt gaatattttg aatatttgtt
    34801 ttattcttaa aaaaaaataa atatatataa ataattatat atatttattt tttttaaaaa
    34861 taaaattaag aggtgatttt atgaatttag attttcacta ttatggcaca tatcttgcag
    34921 ctaaagtagc tggttataac gacactgatg caaagacaat tgcttatgct gctcagtatg
    34981 tagatgaatc agataaaagt atgattcttg atgatgtaaa ctttacactt ccgacaatac
    35041 agactaattt agaatttaaa aaatattatg cagatttggc ttcatggggt tataaatggg
    35101 atttcgagag tttaaatgaa ataaagaaag tatggatacc atttcatttt ttacctggga
    35161 atttaaacaa ccaatataaa tataatggag ttaaagagag taaatgttta acgacaagtt
    35221 ggaaatttaa agatggagat gaccaaaaat ttaggttgat gtgtttacca aatagcgaaa
    35281 ctgtttctgc cattataaat gaccttattt attttcatag taccgaagaa tataagttac
    35341 aatttattgg aatgagaatg catgtacttg cagacacgtg ggcacatatg tattttattg
    35401 gaaaaccaga atggtatatt aatgatgtta aagaatttat agcagaagaa agtaagtaca
    35461 tagaagaaac taaatggact aaagcagaag aatatcgtaa taacggttgg cattctatta
    35521 actttactgg tactcctgat attggtggat atgagagcat atcatatctt ggacatggta
    35581 gaatggggca tataccagac tatggatatc taaattatag atatattcca aactggagta
    35641 gtactgatga taaagtgaag atagaaaaaa ataaccaaga taatttcttt aaggcatttt
    35701 gccaaatggt ttatgcatta aaatgtatta aaaatggaaa tgactttaat ataaatcaat
    35761 atgataacct aacagctcaa caaaaaacag aagtttcaaa agtaatagca acaagaaaaa
    35821 atgaccaatc agaagcatgg aaaaaagcaa tacaggcatt aggatataac cctttagaaa
    35881 aatttgataa aaataagtgg aaaaatgaat ttataaaatc ttctaataaa gagaaaacag
    35941 attactatta ttttaatcta gtagctaaga aacatgtaga ttatgtaact gattttttag
    36001 aggaaaaaag attgtcttta gaagaaatgt tcaatggata ttgtaaattt aaaaattttg
    36061 atgataccca atttgctaaa aatagaaata gagtgacaaa gattatactt agaggagctt
    36121 atatagttga tgctatacaa cttgtatatg atggaaaata taaaactcca atgcatggtg
    36181 aaccatttgg aggaacaatg gtatctcttg atttagatac tgacgattac atagttaaga
    36241 taagtgggtc tataggttta tatgaaggtg gagaaccata tcctaattct ccaactagaa
    36301 caataggaaa aataactttt ttaactaaaa aaggaaaaac aataacagca ggtcatgaaa
    36361 ctatgtttaa attatataga aatttcactt tagaagcacc tgcaggaaaa caaatttttg
    36421 cacttaaagg ttcatattta atacgtaaat taagagaaga gactagcaaa cgatatttgg
    36481 attgtttaga aattgcaaat acaaaagatt gttcggtagc tacaagtacg aaataactac
    36541 atattggata gctagtgtta taatatattt aagaaaatag tataatattt atatttacaa
    36601 tgaaagcact ctttattata aagagtgctt tcattgtaaa tcatcaagaa cattactaaa
    36661 tataccttaa aattgattta aataggtctt tttatttaga aataacagtc atataagtta
    36721 aatattaata tctaaacaat ttaataatga atatagaaag attaaaaggt agcaactaga
    36781 actagttatt accttacttt tttctgattt tttatatct
//
LOCUS       DAMJVM010000032        35599 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.032, whole genome shotgun sequence.
ACCESSION   DAMJVM010000032 DAMJVM010000000
VERSION     DAMJVM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35599
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.032"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            126..266
                     /locus_tag="PC523_001660"
     CDS             126..266
                     /locus_tag="PC523_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475188.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(441..761)
                     /locus_tag="PC523_001661"
     CDS             complement(441..761)
                     /locus_tag="PC523_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDF2475189.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NIDK"
     gene            complement(881..1618)
                     /locus_tag="PC523_001662"
     CDS             complement(881..1618)
                     /locus_tag="PC523_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDF2475190.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDIITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGNYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1832..3652
                     /gene="acd"
                     /locus_tag="PC523_001663"
     CDS             1832..3652
                     /gene="acd"
                     /locus_tag="PC523_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDF2475191.1"
                     /translation="MKKKAALATLAMLPLSVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKLNGDSLRTSTNKEKQVT
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLSKGSKVEVISESNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDDSSQSNDKKNTDKFVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADASNNNSNSNSDSSSSNNGNNSSSGQVNGMS
                     GISGAKIDYKSLNYTLASHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSSF
                     VNASSSDIEYYLNPKNFINTTKGMMQFLKINSYRGGISESSLNSYLNGLSSSVFKNQG
                     ATFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFGI
                     GAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYINSSKYNQNTIYKMKWNYDY
                     TWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3865..8163
                     /locus_tag="PC523_001664"
     CDS             3865..8163
                     /locus_tag="PC523_001664"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDF2475192.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKINKERLIRINDREMEEKIRALEIGKVNSCEESEEEGYVIKSEVDEELIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRKEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHPAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEEDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSVGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFNKIPVATGDVHFLDKHEA
                     ILRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIAEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIIVGEKTLLPPMAAIQGLG
                     GNAAINIQTERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8367..8828
                     /locus_tag="PC523_001665"
     CDS             8367..8828
                     /locus_tag="PC523_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDF2475193.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIVNGKEIEFNRKDVAIVRLAIKF"
     gene            8858..10012
                     /gene="nusA"
                     /locus_tag="PC523_001666"
     CDS             8858..10012
                     /gene="nusA"
                     /locus_tag="PC523_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDF2475194.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKKIEAEDVEAEFIEQEELDTSDDNFSDEE"
     gene            10019..10300
                     /locus_tag="PC523_001667"
     CDS             10019..10300
                     /locus_tag="PC523_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDF2475195.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10290..10601
                     /locus_tag="PC523_001668"
     CDS             10290..10601
                     /locus_tag="PC523_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HDF2475196.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10623..12563
                     /gene="infB"
                     /locus_tag="PC523_001669"
     CDS             10623..12563
                     /gene="infB"
                     /locus_tag="PC523_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDF2475197.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGDELE
                     LALEMLGEDLSQENGNIIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12606..12986
                     /gene="rbfA"
                     /locus_tag="PC523_001670"
     CDS             12606..12986
                     /gene="rbfA"
                     /locus_tag="PC523_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDF2475198.1"
                     /translation="MASYNRTRRISEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKIDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            12967..13950
                     /locus_tag="PC523_001671"
     CDS             12967..13950
                     /locus_tag="PC523_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDF2475199.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNIGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLGFLVEGVKIYKSNEFNMKNYSIIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQIKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNAHASSFEMAKNLLVLGAQKNTIIQNIYQSNSYDYYKLLGEA
                     LKALEIFNSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYIDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            13963..14868
                     /gene="truB"
                     /locus_tag="PC523_001672"
     CDS             13963..14868
                     /gene="truB"
                     /locus_tag="PC523_001672"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDF2475200.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEEIKRA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKNIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNSNAIKYYSNGGSIDDKRFLKNNFNKDDEFVRVYST
                     DNFLGLGKLSKHNNIISVKSDKMFI"
     gene            14998..15927
                     /locus_tag="PC523_001673"
     CDS             14998..15927
                     /locus_tag="PC523_001673"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDF2475201.1"
                     /translation="MEIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMASVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            16031..16288
                     /gene="rpsO"
                     /locus_tag="PC523_001674"
     CDS             16031..16288
                     /gene="rpsO"
                     /locus_tag="PC523_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDF2475202.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            16528..17373
                     /locus_tag="PC523_001675"
     CDS             16528..17373
                     /locus_tag="PC523_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2475203.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFIAEGYHIICMTGAANASGTFQSAMIA
                     KNDVDENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEKIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIITIGCGDNLHDCEILKDEVNKRIKARSVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            17498..19609
                     /gene="pnp"
                     /locus_tag="PC523_001676"
     CDS             17498..19609
                     /gene="pnp"
                     /locus_tag="PC523_001676"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDF2475204.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCIGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            19732..20475
                     /locus_tag="PC523_001677"
     CDS             19732..20475
                     /locus_tag="PC523_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2475205.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDESGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            20633..21880
                     /locus_tag="PC523_001678"
     CDS             20633..21880
                     /locus_tag="PC523_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2475206.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQLNAFTSKECTCYYVKLIDEHIDIGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLVKKEIENIRENYLTEEEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVDINSVKKVVDK
                     VFNVENIGTCIVGRDVEKILHLN"
     gene            21915..22160
                     /locus_tag="PC523_001679"
     CDS             21915..22160
                     /locus_tag="PC523_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HDF2475207.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            22170..23393
                     /gene="dapG"
                     /locus_tag="PC523_001680"
     CDS             22170..23393
                     /gene="dapG"
                     /locus_tag="PC523_001680"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDF2475208.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKIKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKFITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            23507..24241
                     /locus_tag="PC523_001681"
     CDS             23507..24241
                     /locus_tag="PC523_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HDF2475209.1"
                     /translation="MKDYREDNERFDKDNCSEESSSEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            24364..26772
                     /locus_tag="PC523_001682"
     CDS             24364..26772
                     /locus_tag="PC523_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDF2475210.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIVGGWLISIF
                     ALILSVMFIFNISIKDMISNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGRNTDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGTQ
                     SMPELDVLKELQKATNENPVIDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSNY
                     KKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEIQ
                     PSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLES
                     EEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYKA
                     NPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFADA
                     QVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIVA
                     TQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAAK
                     PVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEAI
                     EFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQN
                     LEGE"
     gene            26775..27833
                     /gene="mnmH"
                     /locus_tag="PC523_001683"
     CDS             26775..27833
                     /gene="mnmH"
                     /locus_tag="PC523_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDF2475211.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTIPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNLLVLKECINKF
                     RKRLGHKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYNKVINFNDTEEA
                     LEDVIDLYESILEGENEC"
     gene            27827..29161
                     /gene="rimO"
                     /locus_tag="PC523_001684"
     CDS             27827..29161
                     /gene="rimO"
                     /locus_tag="PC523_001684"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDF2475212.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILRELSEMHQIVSLNDIEFVFNEDLPRYLSTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEDVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEIGEFVSVKINDAMEYDLMGDVLYELA"
     gene            29148..29690
                     /gene="pgsA"
                     /locus_tag="PC523_001685"
     CDS             29148..29690
                     /gene="pgsA"
                     /locus_tag="PC523_001685"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDF2475213.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNIPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVLLLLGAQFANGLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            29957..31003
                     /gene="recA"
                     /locus_tag="PC523_001686"
     CDS             29957..31003
                     /gene="recA"
                     /locus_tag="PC523_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDF2475214.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTNEIDEKVRAFYNLNEEH
                     EEAGNSVSKEIVEE"
     gene            31179..32720
                     /gene="rny"
                     /locus_tag="PC523_001687"
     CDS             31179..32720
                     /gene="rny"
                     /locus_tag="PC523_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDF2475215.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
     gene            33030..35366
                     /locus_tag="PC523_001688"
     CDS             33030..35366
                     /locus_tag="PC523_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2475216.1"
                     /translation="MKKNTLLHTNIMVCIIITLGFVATSIIGYQSNTNIFKNDIEHVS
                     TLAAEGIYYQIDKLLSEPINVSLTMANDSLLKNFLDGEKEHLNDEEFIYKLQDYLNVY
                     RNKYLYDSVFLVSTKTNNYYHFDGLNRNLSVNNSENQWYYTFLNKEDEYSLNVDNDEA
                     SDNSITVFVNCKIKDDNGATMGIIGVGLKVNSLQMLLKGYDDKFDVVTHLIDDKGFVQ
                     LAVDKTGHEHINFFENSSSDLSNSKKLILNNEKEQKSFWYSSEKSKRYIVCQYIPSLK
                     WYLVLENDFTLMSKQLYMQFLKDIGVIVLIIIFVLFTITHVLKKYKKQIVKLSVSQKL
                     EYQKLLSQSTEGLYENIFEIDITNNRAGCEDENRYFHEFGLGLNTPYDEALHTIAKNQ
                     IKEEYAQGYLDTFLPENVIKCYNSGINNISYDFLIKKDEESYHWIRINARIFFWTSDE
                     SVRMISYRKNIDEEKNRELLLLERSQRDSLTGLYNKATTEEFIRSFLKNESNQDLEHV
                     FLIFDIDDFKNVNDKYGHVFGDFVISEFALELKSQFREDDIVGRIGGDEFVVLIKDFN
                     SNNYVLIDKLERFCSKINQKKFAEQAGFSIGCSIGVSMYPEHGYVYSELYEKADQALY
                     YAKSHGKNSYHVFSEDSTDSISFHIDPRDIKVLLEMATDGVVKFACMENLKLLYFNQK
                     MLEFTGYSAQTVTSTDFNIFSPVLQDDLQMVLDTLKEAISKRETFEVVFRFCHTDGHI
                     FPVRMQGKFVSELYQNRYPVFYAIYTDISNINTLLENK"
BASE COUNT        14366 a         3770 c         6729 g        10734 t
ORIGIN      
        1 atatcctaat tatgtcaaaa aataaaaaag tttttattta ttagagtttt tggaaataat
       61 aataacagat attataatta attaacagtt tttgtaagta tttgacaata gagagggtgt
      121 aacaaatggg aataatagag tcagcatcaa aattggctga gatggttcat ttattggctg
      181 ttgaaaaagg gataactgat atagaagcat gggatgaagc tgtgaaagaa tacagcaaaa
      241 tttacgaaga aagaagaaat gaatagaatg ataaaaagtg acaacttgat gataaaaaat
      301 aactctattt aaataaattc aatttttata gaaacattaa aaataatata aataaaaaga
      361 tggtatttca aatgtaagta aaaaatcatt ttgggatacc atccttatta tttattttaa
      421 taatatataa gtaataaatt ttacttatca atatttttta tgtaacctct tttttctagc
      481 ttttttatat gctctttgcg tatttcatca agattaatac catactgttc ttctagctta
      541 aacatcatag taacacatgt ctgagctaca tccattagtt ccttagcagt tttctttata
      601 atatcaactt catctaagtc attattttct ccacttagat ttctaaattt acctatacat
      661 tcagctaatt caccttgttc ttcacccatt ttgataaatg tagattctag ggttgggtct
      721 aaattattaa gctctggtaa agatatagtt tttagtttca taaaagctcc tttcaagtta
      781 ttcatattta tactaaaaca tcataacaaa aataatacta aaaaaaaact ttaaattcaa
      841 cataaaaact tacatatata ataaaaatcg tgaaataaat ctatatttgt cttaatacag
      901 cttcatataa aacttctcca ataggtttat ttattacaag agaagctgac ttatcataga
      961 ctgtatttgc tttattaata agagcaatat aattaccttt gtaataatct ataaaacttg
     1021 ctgctggata tacagccaat gatgtacctc ctataattag taaatcagca ttacttatag
     1081 cagaaattgt tttagtaatg atatcactat caagagcttc ctcataaagt actacatctg
     1141 gttttactat agaaccacag ttatcacagt aaggaatatt accaccaaga tttaacatag
     1201 attctaagtc aaaaaactta ccacatttag tgcaataatt tctatgtaca gagccatgaa
     1261 gctctaggac atttttactt ccagccattt gatgaagtcc atcaatattt tgagttataa
     1321 cagctttaag tttacccatt tcttcgagtt ttgctaaagc tatgtgagca ttgttgggtt
     1381 ttgcattagg gtatattaat ttgtccttat aaaattcaaa aaattcttct gggtatctaa
     1441 caaaaaaagt atgtgaaact aattgttcag cagtaaattg tttgttgagt ttttggctaa
     1501 ataatccagt agagcttcta aagtctggaa tattagattc agttgaaaca cctgctccac
     1561 caaaaaatac aatattattg tgattagcta ttaagtcttt taagctattt gcatccatag
     1621 ttacacctcc aaattattat atataaattg tactacatat taaggtattt atgttttatt
     1681 atttcaaata aatacatagt ttaataagtt aaataaaggg aatataaata aaatgtatac
     1741 ataatataaa taggcaatat ttactaaaat aacaagttgt cgtataatta tattaaaatt
     1801 gttttagtgt ataagaaggg cggggaagta catgaagaaa aaagcagctt tagcaacatt
     1861 agctatgtta cccttaagtg tggtaaatgc acatgctgat ggagatatag gcatagtgac
     1921 tataaattat ttaaacgtaa gaaatgaacc aactgctgaa agtagtatag cctttgttgc
     1981 taaaaaagat gataaagttt tgattaaaga ctcttctaat ggatggtata aaataaaagc
     2041 tgaatctgga caagaaggtt gggcttcatc aaaatatata gcaaaattaa atggtgactc
     2101 tttaagaaca tctacaaata aagaaaaaca agttacttca aatagtttaa atatgagaaa
     2161 tggagcaggt acaagttata gagtaataac tgtactaaaa aaaggtcaaa aagtagaagt
     2221 aatatcagag agtaatgggt ggtctaaaat caaatatgat ggaagattag ggtatgtatc
     2281 tagttcttat ttaggagatg tttcaaattc aactaataaa tcaaaaacaa aacaagtgaa
     2341 tacaacttca ctaaatgtga gaagtggacc aaatacaagt tatggcttgt taggtaagtt
     2401 gtcaaaagga agtaaagtag aagtaatatc agaaagtaat ggatggtcca aaataaagta
     2461 taatggaaaa gatgcatatg tatctagtat gtacttatca gatgtaagtc aaagtaattc
     2521 agacgattct agtcaaagca atgataaaaa aaatactgat aagtttgtaa atacagcttc
     2581 tttaaacgta agaagtggac caggttctac atatagcaag ttgggaaagg tttataaagg
     2641 aagtaaggta actgtgctat cagaaagtag tggatgggct aagattaatt ttaataataa
     2701 agaagccttt gtagtaggta attatttatc tacttcagca gatgcatcaa ataataactc
     2761 aaatagtaac tctgatagca gttctagtaa taatggtaat aattcatcat caggtcaagt
     2821 aaatggtatg tcaggtataa gtggagctaa aattgattat aaatctttga attatacttt
     2881 ggcatctcat ataagtaaac aagttgagaa agcagcatca ggaggaaatg tgatagcacc
     2941 aagtaatagg aaaagcaccc caagtcctga atttagtact ttttcagccc aaagaacaag
     3001 ctcatttgta aatgcaagtt caagtgatat agaatattat ttaaatccta aaaattttat
     3061 aaatactact aaaggtatga tgcagttttt aaagattaac agttatagag gtggtatttc
     3121 agaatcaagt cttaactcgt atcttaatgg tttatcttct agtgtattta aaaaccaggg
     3181 agcaacattt ataaatgctg caaagaagta taatatagat gttgtatatc tagtatctca
     3241 tgctatgtgg gaaactgctt atggtaagtc tacacttgct caaggtcaga cattaacatc
     3301 ttataagggt caacctctta gtaaaccagt taaagtatac aatttttttg ggataggagc
     3361 gatagataaa agtgctaatg tttcaggtgc agaggcagcg tattccaatg gttggactag
     3421 tgtagaagct acaatagatg gatctgcaaa gtggatatca caaaattaca taaacagttc
     3481 taagtacaat cagaatacta tctacaagat gaagtggaat tatgattata catggcatca
     3541 atatgcaact gatgtaaact gggctaatgg gatttctgga attatggaga atttaattgg
     3601 tctttatgga ggaggaagta gtttggtttt tgaagttcct caatataagt aatatatgaa
     3661 taaaaaatat agactatggg tagttttcat tttatttaaa ttatctatag tctttttgta
     3721 tataatttac atgctgtaaa tttaatgtta acaaaatttt tatgctataa tattaattct
     3781 aagcaaatat aagtgagtaa tagatagaaa tagataaaaa gtggtaataa tatatataga
     3841 taattttgag aaaggaggat atagatggaa agtattaagg aatatttgga taagttagaa
     3901 attaataata gtggtcttgg aaagcaactt aaagaagttt acataaatag agtagtttat
     3961 tttaaggaag ataagatagt ttatttttat ctgacttcta aggatattgt ttctcatgag
     4021 cttttagata aatttaaaga agaattgatg tataaactcg attattttaa agatatgagg
     4081 atgaagataa gatttactgg tcttgaaaga aaatccaata aagatgttat aaaaaaatat
     4141 tggaataata tattgtatat attaaagtat ctttgtccat ctattgcagg atggtataaa
     4201 caggtagaat ttctttgttt agaggaagaa ttaaaaataa aacttccaaa aggcatattt
     4261 tatgagcgat taatgaaaaa aaatgtaacc tatgtactta aaaccgtgct tagtgaagaa
     4321 ctgggattag atttaaatat aacaatagaa aaagcggttg atgaaaagat aaataaagag
     4381 agactcataa gaatcaatga tagagaaatg gaagaaaaaa ttagagcttt agaaattgga
     4441 aaagttaata gctgtgaaga aagtgaagag gaaggttacg tcattaagtc tgaagtagat
     4501 gaagaattaa tctatggaga taatgcaaat gctatgattg aaaatatagt tgaattaaat
     4561 gcatcttctg gaactgttgc agttgttgga gatatatttg atgttgatac taaagagctt
     4621 aaaaatggaa aaatacttat gatagcatca attacagatt atactagttc aataagttgt
     4681 aaactttttt taacagatac aaataaagat ggagtacttg aaaatgttaa aaaaggagca
     4741 tacttaaaaa tcaagggtga catagtttat gatacatatc aaagagaaat tagtatgatg
     4801 attagtggta taagaaaaga aactagaaaa gagagagaag ataaatcaga ggaaaaaagg
     4861 gtagaactac atgcacatac tcaaatgtct tctatggatg ctatttgttc tgttaaaaag
     4921 cttgtagaac gtgcggctaa atggggacat cctgctattg ctataacaga ccatggagtt
     4981 gtacaaggat ttccagatgc catgaatgca ggtaagtcaa ataatataaa aatactttat
     5041 ggagtagaag ggtacttggt tgaagaggat tctcttatta tagaggatgc aaatgataaa
     5101 gaactttctc agacatttgt tgtatttgac atagagacaa ctggtttttc taatacaaat
     5161 gataaaataa ctgaaattgg tgctgtaaaa atagaaaact ttaaaattgt agatagattt
     5221 agtgaattaa taaaccctga aaaagatatt tcttataaga tacaggaatt gacgggaata
     5281 actaatgagc taattaaaga taagccgacc attgaagaag ttcttcctaa gtttatggaa
     5341 tttgttggag atagtgtatt agtagcacat aatgcggaat ttgatactgg atttatatcg
     5401 caaaaatgta gggaacaagg tttgttatat aataacaaaa aagtagatac acttatgcta
     5461 gcaagagtaa tgttaccaaa cttaaaaaga catagactaa atgtcgttgc aaaagaactt
     5521 ggtattcctc ttttaaatca ccatagagca gtggatgatg ctgaggctac agcacttata
     5581 tttaataaat ttttgcagat gcttacggaa aaaggtgcaa aaacattaag tgatgtaaac
     5641 aatatacttg gaaagattga ttatacaaaa cttagcacaa atcatataac cttgatagca
     5701 aaaaattctg taggtataaa aaatttatat aaaataattt cagatgcaca tgtaaatcat
     5761 ttttttagag caccaagaat ccttagaagt gtgttaaatg aatataaaga aggattaata
     5821 attggttcgg cctgtgaagc tggagtggtt tttcaagctg taaagaaaaa tgtatctgat
     5881 gaggaaatga aaaaaataat agatttatac gattatattg aagtaatgcc tatagataat
     5941 aataggttta tgattgataa aggagaagta aaggacgaag aagagttaag ggaattgaat
     6001 aggaaattaa ttgatacagc taaaaaattt aataaaattc cagtagctac tggggatgtt
     6061 cattttttag ataaacatga agctatctta agaaaagttc ttaaatattc tcaaggtttt
     6121 aaagtagatg aagaagaaac ttatttacat tttagaacaa cagatgaaat gttggaagag
     6181 tttagttatc ttggagaaga attggcatat gaagttgtag ttgaaaatag taatctgatt
     6241 gccgatatgg ttgaagatat aaaacctata ccagatgata cttatcctcc aattatagag
     6301 ggttcagatg tggagttaag agaaatgtgt tacaaaaaag caaaacgaat ctatggagac
     6361 ccaataccag aaatagttca aaaaagactg gatagagaat taaattcaat aataagcaat
     6421 gggtatgcag ttatgtatat tatagctcaa aagctagttg caaaatctct ttcagatggg
     6481 tatttagtag gttcaagagg ttcagttggt tcatcttttg cagctactat gagtgatata
     6541 acagaagtta atcctcttcc agcacattat atttgtgaaa atgaagactg taaatattca
     6601 ttcttttatg aaataggtga atggggttca ggtgttgatt taccagacaa agactgtcca
     6661 aagtgtggaa gaaaacttgc caaaaacggt catgatattc catttgaggt tttcttaggt
     6721 tttgaaggtg ataaggaacc agatatagac ttaaatttct caggaaatta tcagccaacg
     6781 attcacaaat acacagaaga actatttgga gagggctatg tatatagggc tggaacaata
     6841 ggtactgtag cagaaaaaac agcttttgga tatgcaagaa aatatgtgga ggaaaataat
     6901 ataagtgtac caaatgcaga agtacttagg ctttcaaatg gatgtactgg agtaaaaaga
     6961 acctcaggac agcatccagg tggagttatg gttataccag attataaaga tgtgtatgat
     7021 tttacaccta tacagtatcc agcaaacgat acttcttgtg gtgttataac tactcacttt
     7081 gattatcatt caataagtgg tagaatactt aaacttgata tacttggaca cgatggtcct
     7141 accattataa ggatgcttga ggatatgact ggaataaata taacagaaat tccgttggat
     7201 gataaagaga ctatgtctct atttacatct acagaagcat tgggtgttac accagaagaa
     7261 ataaactgtc ctataggttg ccttgctgta ccagagtttg gaactaagtt tgtacgacaa
     7321 atgttacttg acaccaaacc tactacattt gcagaacttg tacgtatatc tggtctttct
     7381 catgggacag atgtttgggt aaataatgca caagacttag ttagagaaga tatagttggc
     7441 ttaaaagatg ttatatctac acgagatgac ataatgaact atttaatatt taaaggatta
     7501 cctcccaaaa tgagttttac aataatggag agcgtaagaa aaggtaaagg tctaaaacca
     7561 gaacacattg cggagatgaa aaaaaataat gttccagaat ggtatatagg ctcatgtaaa
     7621 aagattaagt atatgttccc aaaagcacat gctgttgctt atgttatgac atcatttaga
     7681 attgcatatt gtaaagttca ctatccagag gcattttatg cgacttattt tactacaaaa
     7741 gttgaggatt ttgatgcaga tttaattgtt aaaggattag atgctataaa atcaaaaata
     7801 aatgaaattg agtcgctggg aaatgatgca accacaaaag aaaaaggtat gttgactgtt
     7861 ttagaagttg ctcttgaaat gtatgctaga ggaataaaat tattaccagt tgatatatat
     7921 aaatcagatg cgactgagtt tataattgta ggagaaaaaa cattacttcc accaatggca
     7981 gcaatacaag gtctgggagg aaatgcggct ataaacatcc aaactgaaag aaaaaatggt
     8041 gaatttatat caaaagaaga tttaagaaag agaacaaaaa tatcaaaaac ggtaatagaa
     8101 actcttacta atcatggttc acttgagaat atgagtgaga gaaatcaatt gtcattgttt
     8161 taagttagcc caaaaagttg caaataaaag aattttatga tatcatagta tagaaaatag
     8221 tatactttgc agaaactaag agtgagaccg ctgttctcac tcttttattt taaatatatt
     8281 attaaaaagc tggtttaatt ttattaaata tattttgttt aagattatat aaactggtca
     8341 tttacaattg aataggagga attttaatga aaaaaaattt agaagctaca atcgaggaga
     8401 ttgtaactaa aataacagat gagcatggat ttgaaatggt tgatgttgaa tatgtaaaag
     8461 aagcaggaga atactactta agagtataca tagataaaga agaaggaata tctttaaatg
     8521 aatgtgaatt agttagtaga gaattaagtc caatactaga tgaaaaagac cctataaaag
     8581 agaattactt tttagaggta tcttctccag ggcttgatag agcattaaaa aaagacagag
     8641 attttgttag atatcaaggt agagatgttg atttaaaatt atataaacca ttaaatggat
     8701 gtaaacaatt tgaaggtgaa cttgtaggat taacagaaga taataatata aaaatcattg
     8761 taaatggtaa ggaaattgaa tttaatagaa aagatgttgc tattgtcaga ttagcaataa
     8821 agttttagtg gtatattaaa tagggaggat ataaaaaatg aatcatgaat ttatggaagc
     8881 attagatgaa ttagtagttg acagaggtat agataaagaa atacttatag atactataga
     8941 acaagctctt ttaactgcat acaagaaaaa ctttggttca gcacaaaatg ttagagttga
     9001 atttgataga gacaagggag atataaaagt attttctcaa agagtagttg ttgatgaatc
     9061 agatttatat gatactttct tagaaataga gttagcagat gctagagaga taaatcctaa
     9121 ttatgagtta ggtgatatta tagaacatga agttacacct aaaaactttg gaagaattgc
     9181 agctcaaaca gcaaagcaag tagttgttca aagaataaga gaagctgaga gagaaatagt
     9241 gtataatgag tttatagaaa aagaaaatga aatagttaca ggagaggttg ctagagcaaa
     9301 taaaaatgtt gtttatgtaa accttgggag aatagaaggt ataatgactc aaagtgagca
     9361 aataccattt gaaaattata aagcaggtca aaaaataaaa ttctatatac ttgaagttaa
     9421 aaaaactaac aaaggacctc aaatagttgt atcaagaagt catccaggtt tagtgaagag
     9481 attgtttgaa gaagaagttc ctgaaatata tgaaggaata gttcaaataa aatctgtagc
     9541 aagggaagct gggtcaagaa ctaaaatggc agtaagatct atagatgaaa aaatagaccc
     9601 aattggagct tgtgttggac caaaaggact tagagtaaaa aatatagtgg atgaacttgg
     9661 cgatgagaaa atagatataa taaaatatag tgaagaccca gcagagttta tatcagcagc
     9721 acttagccca tcaaaagttg ttagagtaga tatagatgaa gatgaaaagt ctgccttagt
     9781 aatcgttcca gattatcagt tatcgcttgc aataggtaaa gaaggtcaaa atgcaagact
     9841 agcagcaaaa ttgactaact ggaaaataga tattaagagc gaatctcaag cagaacaaga
     9901 aatgttaaat cttgaaaatt ccaaaaaaat agaagctgag gatgttgaag ctgaatttat
     9961 cgaacaagaa gagttagata cttcagatga taattttagt gatgaagagt aaggagggat
    10021 gattttgaaa aaagtaaaaa aagtgcctca aagaaaatgc atagcatgtc aagatagaga
    10081 ctctaaaaaa gagcttataa gaatagttaa aaataaagaa ggaaaaatat ttttagaccc
    10141 aacaggaaga gccaatggaa gaggagcata tgtatgtaaa agctctgaat gtctaaaaaa
    10201 agctataaag agtaaagcat taaacaaagc ttttaagata gatgttcctg atgaagtcta
    10261 tgataatcta cttatggagt tagaagaata tgaaaaataa taaagaaaaa gtactttcgt
    10321 tcttagggct cgcaactcgt gcagggaaaa tagtgtctgg tgatgattca actttgctcg
    10381 accttaaaaa aggaaaagta aatttgattt taatagcaga agatgcatca aataatacta
    10441 aaaaactttt taaagataaa tcaactttta gaaatattcc atatctattt ttttcaacaa
    10501 aagaggaaat aggatttgca ataggaaaat ctccaagagc agttgtagga ataaaagatg
    10561 aaaatttttc taaaaagata attgaactaa tagaaattta aaataaaata gggggtgatc
    10621 ttgtgtcaaa aacaagagta taccaaatag cagaagaatt aaatatatca aatgaagagc
    10681 ttataaataa gttagctgaa ttagatataa atgtaactga caaagattca gtattagaag
    10741 gtgatgagtt agaattagca cttgaaatgt taggtgaaga tttatctcaa gaaaatggaa
    10801 atattataga aatagatgga aagttgacag tacaagtatt agcaacaaaa ttagataaat
    10861 ctccatcaga aataattatg aagcttatga aaatgggaac tatggcaaca ataaatcaag
    10921 agataagttt tgagattgct gctttggctg ccaaagacta tggatttgaa ttaacagtag
    10981 ctgaaagtga tgatacagaa gctttagaaa tagaagcttt aatggaaatt gaagaagata
    11041 aagaagagga tttaaaacct agaccaccag ttgttacagt tatgggacat gttgaccatg
    11101 gtaagacatc tttactagat gcaataagaa aaactgatgt aatctcaggt gaagctggtg
    11161 gaataactca gcatataggt gcttcagagg ttaaaatcaa tggacacaaa atagtatttt
    11221 tagatacgcc aggacatgag gctttcacat caatgagagc aagaggagca caagttacag
    11281 atatagcaat attagtagtt gcagcggatg atggtataat gcctcaaaca gttgaagcta
    11341 taaaccatgc taaggcagca ggtgtacctt taatagttgc tatcaataaa atagataaac
    11401 caggtgcaaa cccagataaa gttaaacaag aattagcaga ccaaggatta ttagttgaag
    11461 actggggtgg agaagtaata gcagttccag tatcagctaa gaaaaaagaa ggaatagata
    11521 cacttttaga aatggtatta ttggttgctg aaatggaaga gttaagagct aatccaaata
    11581 aaagagcagt aggaactgtt atagaagctg agcttgataa aggaagagga ccagttgcaa
    11641 cagtacttgt tcaaggtggt actttaacag taggtgaccc aatcgttgca ggtgttgcat
    11701 gtggtaaagt tagagcaatg ataaatgcaa aaggtaagag agtaaaaaca gcaggaccat
    11761 ctacagcagt tgagatatta ggtctatcag aagttccaca aggaggagac caatttgtcg
    11821 aagttccaac tgataagatt gctagaagtg tagcagcaag aagacaacaa atagttagag
    11881 atgaaatgtt aaaatcaact caaagattat cactagatgc tttatttagt caaatgagtg
    11941 aaggttctat aaaagacctt aatatagtta taaaagcaga tgtacaaggt tcagtacagg
    12001 cagttaaaca atctttagaa aaattatcaa atgaagaagt tcaagttaaa gtaatacatg
    12061 gtggtgttgg agctgtaaca gaatcagata tattacttgc agcagcttct aacgcaataa
    12121 taataggatt caatgtaaga ccagttccag gtgcagaatc tctaggagaa aaagaaaatg
    12181 ttgatataag aacatatact ataatataca aggcaataga agatatacaa gctgctatga
    12241 ctggtatgtt agaccctgaa tatgttgatg aagaaacagg aaaagctgaa ataagagaaa
    12301 tatataaaat atctggtgtt ggaacagtag ctggatgtta tgtgactaat ggtaagatat
    12361 ttagaaattg taaagtaaga ttagtcagag atagcataat aatacatgaa ggtgagcttg
    12421 cagcacttaa gagatttaaa gatgatgtaa aagaagttaa ttctggatac gaatgtggta
    12481 tgagttttgt aaattataac gacataaaag aaggcgatat agtagaagct tatataacta
    12541 aagaagtaga aagaaaatta taaattcgta tcaaagaaaa tcagttagta aagaaggtcg
    12601 atggtatggc atcatataat agaacaagaa ggatatcaga agaaataaga aaagtagtaa
    12661 gtactatgct tataaatgga gtaaaagacc ctagaataac atctatggtg agtgtaactg
    12721 atgtagaagt tacaaatgac ctaagatatg cttatgtata tgtaagtatt ctaggtggag
    12781 atgaggaatc cacactaaca ggtcttaaaa gtgctggagg atatataaga agagaagtag
    12841 gaaaaaatat aaaacttaga tatattcctg agatagtatt taaaattgat gattcaatag
    12901 aaaaaggaat gtatatggat agccttataa aaagagttaa tgaaaaaaat gctcaacaaa
    12961 atgaggatga aaattatgat gaataatatg gatagtatta taaactatat ttatgagggt
    13021 gactatttta tagttacctc tcatatcagt ccagatggag ataatattgg ctctacactt
    13081 tcaatgtatt atacattgaa gaatttagga aaaaatgtat attatgtact tgatgatgaa
    13141 gcaccattaa atctaggatt tttggttgaa ggtgtaaaaa tctataaatc taatgaattt
    13201 aatatgaaga actattctat aattgcactt gattgtggag ataagatgag aatatgtgtg
    13261 agtgatgaaa tcaaaaataa tgcatcaaaa ttaatttgta ttgaccatca tgctagtaat
    13321 gattcatatg gagatttaaa ttatgtagat attgatgcat cttctacatg tgaactagtt
    13381 tataatttat tggttagatt ccagcaaata aaagatatta atattatcaa tgaagatata
    13441 gcaacatgtt tatatacagg tcttgtaact gatactggaa acttttcata ttcaaatgca
    13501 catgcaagta gttttgaaat ggcaaaaaat ttacttgttt taggtgcaca aaaaaatact
    13561 ataatacaaa atatatatca aagtaattca tatgattatt ataaattatt aggagaagct
    13621 ctaaaagctt tggagatatt taattcaaag gtatctagca tagttttaac tcaagacatg
    13681 atgaatagaa ataatattag ctttaatgat gttgatggaa taacaacata tacaagagat
    13741 ataaaaggta ttgaagttgg aattttattt aaagagaaaa aacaaaatga aattaaagta
    13801 agttttaggt caaaaaatta tattgatgta agtgaaatag ccaagttatt tggcggtggt
    13861 ggtcatgtaa gggctgctgg atgtactatt agagatagta ttgataatgc aaaaaaaatg
    13921 gtactagagg cagtgttaaa atcaatttaa ggtgacagta aaatgaataa gattataagt
    13981 atattgaaac ctacaggtat gacatctcat gatgttgtaa gtagagtgag aaagatttta
    14041 aatataaaaa aagtcggtca tacaggtact cttgacccag atgcttcagg tgttttacca
    14101 atctgtatag ggaaagctac aaaggtatgt gaagttattt taaacaaaga taaatcgtat
    14161 atatgtgagc ttaccttagg tataagtaca gatacttatg atgcctcagg tgaaattcta
    14221 aaaaaagttg atgattttaa atttagtaat gaagagataa aaagagcttt tgatacacaa
    14281 agaggagaga taaatcaact tcctccaatt tattcggctt taaaggtaaa tggaaagaga
    14341 atgtgtgatt tagtaagaag tggtagacaa tctgaaatca ctttaaaaac aagacgagta
    14401 aatataaaaa acataaaaat actaagtata aaaggtaata aagtaatgtt ttatgttgag
    14461 tgttctaaag ggacttatgt gagaagtatt tgccatgata taggagaata tctaggttgt
    14521 ggagctcata tgtcgttttt gaatagaaca tcttctggta aatttgattt agacaattca
    14581 ataacattag aagaattaga attattttat gaaaataaaa ctttagacaa atacttatat
    14641 gatatagatt atgtacttga ttcatttaat tatgttgtat taaactctaa tgctataaaa
    14701 tattatagca atggtggttc aatagatgac aaaaggtttt taaaaaataa tttcaataaa
    14761 gatgatgaat ttgtaagagt atacagtaca gataatttct taggattagg aaagttgtca
    14821 aaacacaata atataataag tgttaaaagt gataagatgt tcatataaat aatgtattta
    14881 aaaaacaaca aaagagatag tactataata acttctatag tctatctctt ttacttatgc
    14941 tataatagtt tgtgaataag ttaagagtaa attatatttt agcggggaac atttattatg
    15001 gagattataa aatctataaa agaaatagca aacataaaag aaagtgtcat taccatagga
    15061 aattttgatg gtgtacacaa aggacatcaa gttcttatag gaaaaacagt tgaacatgca
    15121 aaaaaagaaa atatacagag tattgtattt acatttgcaa atcatcctgt aaattacttt
    15181 agaccaaact caacaaaaaa tattataagc aatgatgata aaataaaatt attagaagat
    15241 ttgggaatag atatagttgt aatcatacca tttgatgagt atatgactaa aatacctgca
    15301 aaggattttg tagatgagat actagtaaaa aaattaaaag ctaaaaagat agtaattgga
    15361 catgacttta catttgcaag ggcaaaagaa ggcaatgtta atctattaaa aagcttagaa
    15421 catgaatttg gttttgaggt agaagtaata aaaccaatca aaatcaatga tgtaagagtg
    15481 agtagtactt atattagaag tttagtatcc cagggtgata tggctagtgt caaggaatat
    15541 ttgggaagaa attacaagtt agaaggttgc gttatacatt ctaaacatct tggaagaact
    15601 ataggattcc caacagcaaa tattgacctt aagaataata tgctcgttcc aaagagaggt
    15661 atttatgctt ccattgtaca tattggagat gagacttatt ttggtgctac aaatgtaggc
    15721 tataacccaa cagttaatgg aaaaactttg tctattgaaa caaatatttt agagtttgat
    15781 agagatattt atggagaaaa tataattgtt gaatttttgg aaagaataag agatgaaaga
    15841 aagtttaatt caatagatga cttaaaaaat caattgtata atgatacaaa ttatgtgtat
    15901 gaaaactatg tttgcaaaaa caagtaatgt atgataaaat gattaaggaa aataaccatt
    15961 actcggcatt tgaatactcc aatcaatgct tagtaatagg cgtttctaaa aaaatacgga
    16021 ggtaatctaa atgataaaca aatcagaaat aataaaagaa tatggaagag ctgaaggaga
    16081 tactggttct ccagaagtac aaatagcttt attaacagct agaataaacg agttaaatgg
    16141 acatttaaaa actcataaga aagaccacca ttcaagaaga ggtctattaa aaatggttgg
    16201 tagaagaaga aacttattag cttacttaaa ggaaaaagat cttgaaggat atagagcatt
    16261 aatagctaaa ttaggattaa gaaagtaatt tcattcttta taaaaaagca ggtatagtgt
    16321 atacctgctt ttttatagtt aattcattat aaagtaaaat agttaataat aaaagtagtt
    16381 tatatgttat aaaaaattat aaaatgaata atattgggta agaatattaa aatagtatac
    16441 aaatacataa tcaatttaaa aggaaagata ggacaaatat agaaatttta taacaggata
    16501 attagttaga ataaggagga tattattttg aaaataaagt tagtatgtga tagtttatgc
    16561 gatattccag atgaaatatc agaaaaagat tatatagaga tagttccact tacagttatt
    16621 tttaatgata gggaatatat agaaggagta gatataaaga aagaagagtt ttataaaaaa
    16681 gtaaaagaaa ttaaacaaat acctaaaact tcacaagcta catatatgga gtttaaagaa
    16741 gtatttgata agttcatagc agaaggttat catataatct gtatgacagg ggctgcaaac
    16801 gcatcaggaa cttttcaaag tgctatgata gctaaaaatg atgtagatga aaatgaaaaa
    16861 atacatatat ttgatactag aaatttatct ctaggtagtg gacaatatgt tataaaggca
    16921 tgtgaacttc ttgaagaagg actgggattt gaaaaaatta tagatgaatt agaaaatact
    16981 cgaagtagtg taagattact ttttgcacca tatacacttg attttttaaa gcagagtgga
    17041 agagttccag ttgcaactgc tttgatagga aatatgttaa atataaagcc tatatttttc
    17101 tttgataatg gggaagctaa gttggtaaat aaggttagag gtataaaaaa tatagcaagt
    17161 aaattggtcg atataatttt agaaatgaat gaagggtctt tagaaggaaa aatcatcaca
    17221 ataggttgtg gagataattt acatgattgt gaaatattaa aagatgaagt aaataaaaga
    17281 ataaaagcaa gaagcgtatt atttactcga ggaggagttt ctatttgctc tcatacaggg
    17341 ccagatatat tggctataag ttgttctaat tagtagtaat ataaggtttt tgcatatata
    17401 tgtgaagata attttatatt gttaatttat agtatttttg ataatatagt aaatagagtg
    17461 atatacgtgt tttttaataa gaggaggaca agcattaatg tttgaacata aaatatttaa
    17521 aatggatttt gcaggaagag aactttctgt agaaatagga aagatttgtg aaatggctag
    17581 tggtagttgt atagttcgat acagtgacag tatggtaatg gtaaatacaa ctaaatcagc
    17641 caaacctaga gacggaatag acttttttcc tttaagtgtg gattatgaag aaaaattata
    17701 ctctgtaggt aagatacctg gaggtttttt aaagagagaa ggtaagcctt cagaaaaagc
    17761 tatacttaca tcaagattga tagatagacc aataagacct ttattcccaa aaggatttag
    17821 aaatgatgtt caggtagttg caacagttct atcagtagac caagattgca caccagatat
    17881 agtagcaatg ataggttctt caatagccct ttctatatca gacataccat ttaatggacc
    17941 aacaggttca gtatgtattg gccttgtaga tggtgcattt gttgtaaatc caaacgcaga
    18001 gcaaagagaa aaaagttcaa tgcatctagt tgtttcaggt acaaaggaag caataatgat
    18061 ggttgaagca ggagctgacg aagttccaga tgaggtaatg cttgatgcta ttttatttgc
    18121 tcaccaagaa ataaaaaaaa tagttgagtt tatagaaggt atagttgcag aagttggaaa
    18181 agaaaaaatg cctgttgaat tataccatgc aggagaagaa ataactcaat tggttcgtga
    18241 atttgcaact gataagatga aaaaagcagt tcagactttt gaaaagttag agagaatgga
    18301 aaatatggat agagttaaag aagaaacttt agctcacttt gaagagactt tagaagattt
    18361 tgaagatttt gttggagata tagaagaagt attacaagat ataataaaag aagaagtaag
    18421 aaaacttata gtacatgaaa atgtaagacc agataataga aaacttgaag aaataagacc
    18481 tatatggtgt gaaactggca tgatacctag agctcatggt tcagccatat ttactagagg
    18541 acaaactcaa gtacttaatg ttgctacttt aggtgcttta ggagatgttc aaaaattaga
    18601 tgggttagat gaagaagaaa acaagagata tatgcatcat tataatttcc ctgcatatag
    18661 tgttggagaa gctagaccat caagaggacc aggaagaaga gaaataggac atggtgctct
    18721 agcagaaaga gctttattac cagtaatacc atctcaagaa gaatttcctt atgctattag
    18781 attagtttct gaagtattaa gttcaaatgg ttctacatct caagcaagtg tatgtggctc
    18841 aacattatct ttacttgatg caggtgtacc tataaaagat atggttgcag gtatagcaat
    18901 gggacttata aagcatgatg gtaaggttgc agtgctttca gatatacaag gtatggaaga
    18961 ccatttaggt gatatggact ttaaagtagc tggaacagag tatggtataa cagcaataca
    19021 aatggatata aaaattgatg gtatagataa agaaattcta caacgtgctt taaaacaagc
    19081 taaagaaggt agaatccata tactaggaga gatgagaaaa actatatctc agccaaaacc
    19141 agaactttca ccatatgcac ctaagattgt taaaatgcaa ataaatcctg acaagataaa
    19201 agatgtaata ggacctggtg gaaagataat aactaagata atagacgaaa ctggagttaa
    19261 gatagatatt gaacaaactg gggaagtatt tatatcaggt attgagattg atatgattaa
    19321 gaaagctcaa gaacttataa ataatatagt agtagaacca gaagtaggaa aaacttataa
    19381 aggtaaagta agtagaataa tgaactttgg agcatttgtt gagatacttc caggaaaaga
    19441 aggattactt catatatctc atatagctca tgaaagagtt gctaaagttg aagatgtatt
    19501 gaatataggg gatgaagttg aagttaaagt tacagagatt gatgaaaaag gaagagttaa
    19561 tttatcaaga aaagttcttt taccaaaacc agagcataaa aataaataat ttattaaata
    19621 ataaaaagca aagtcttagg attttgcttt ttattatttg gtgcataaac agagcttatt
    19681 tttaatagta ttaaataaga aacaatttgt acataattag ggggagtttt aatgataatg
    19741 atggtactta ataagagtaa gctcaaaaaa atcataatac taattttagt agtgttatta
    19801 gtattatcag gaggggtaat ggtattaaag ttatttaaca acaagccagt atttaattta
    19861 tttaatggtg tagatttacc atatgaaaga ggtacaaagg atgaaagtgg atacgttgca
    19921 ttaacctgta atatagacct tggatgggaa acagagtacg tagaatccat tttagaaaca
    19981 cttaaaaaag aaaatgtaaa gattacattt aatgtaaccg gaaaatgggc agaaaagaat
    20041 aaggacgaat tattaaagat aaaaaaacaa ggacatgaaa taggtaatca tggatacaaa
    20101 catttagatt actctacatt atcctatgaa gacaattatg agcaaataga gacctctaaa
    20161 aagataattg aagaaattat aggtgaaaaa actaaatttt ttcaagcacc agcaggttct
    20221 tttggtccag aaacagtaaa agctgctaaa gcattagggt atacttctat caaatgggat
    20281 gccgatacta tagactggaa atacaaagac caaccagagg taatcattga tagaatgaag
    20341 aaaaaagata ttaaagatag tagtataatc ttaatgcacc caaccaatgc aacgacaaaa
    20401 tgtatagatg atattatagc tattgtaaga gaaaaaggac taaaaccagg aaagcttagt
    20461 gatgtattta agtagacaaa gtataaatat ttggttaaga ttgtataagt tgtagttgaa
    20521 ttaattaaaa gactgactta taatgtaata catataaaaa ataaatataa ttaaaacata
    20581 gtgttttcaa taatccaata tgaaacaaag aataaattgt taggagaatg taatgtataa
    20641 gacaaaaata ctggaaaatg ggcttacaat aattggtgaa gaaataccat atctaaagtc
    20701 aataacatta ggtatttgga taaatgcagg ctctaggata gaagaagcac aggtgagtgg
    20761 aacttctcat tttatagagc atatgatgtt taaaggaacc aaaaacagaa catcaaaaga
    20821 gattgctagc tcaatagata atttgggtgg tcaactaaat gcatttacaa gtaaagaatg
    20881 tacatgttat tatgtaaaac taatagatga gcatatagat ataggaattg atgtattaag
    20941 tgatatgata ctaaattcta aatttgataa aaatgatatc gataaggaaa gacttataat
    21001 attggaagaa cttaagatgt atgaagattc tccagatgac ttatcttatg atttattagt
    21061 ggaaaatata tatgctaatg atggattagg aatgaatata attgggacaa aagaatctct
    21121 atacaatata actagagagt ctatgttaga atacttaaat aagtattata ttccaaataa
    21181 cgctgttatt tctatagctg gaaactttaa ctttgatgat atggtagaga aaattaaatc
    21241 aaaatttgga cactgggaaa agaaaaattt gagtatagac ataagtgagg ctaagtttaa
    21301 tccttgcttt atatcaaaaa ataaagatac tgaacaggtg aacttagcta tgtgcctaaa
    21361 aggtattcct tttgaaaatg atgaagaagt ctactcaatg gcagtggtaa ataatatttt
    21421 tggtggaagt ataagctcaa ggctttttca gaaaataagg gaagaaaaag gtttagtata
    21481 ttcaatatac tcatcacaga cattatatag aaaatgtggt gaattaggta tatttgcaag
    21541 tatgagtaca gaaaatttac aagatgtgta taatttagta aaaaaagaaa ttgaaaatat
    21601 aagagaaaac tatcttaccg aagaagaaat atctgaaagt aaagaacagt taaaaggcaa
    21661 ttatatattg gatttggaaa gtactagcag tagaatgatg tctactggta aatctatgtt
    21721 acttagtaaa aaagtaaaaa ctacagatga aatacttgaa tgtataaata atgttgatat
    21781 taattcagta aagaaagttg ttgataaggt tttcaatgtc gagaatattg gtacatgtat
    21841 agttggaaga gatgtggaaa aaattctaca cttgaattaa tattgtaaaa agtaaatctc
    21901 tggaggtata aaatatgaat ttatctgaaa tagcaggtaa ggaaatagtt aacttggtca
    21961 caggagaaag attgggggtc attggagaat gtgaccttat actagatgaa actacaggcg
    22021 gtatattagc gcttctaatt ccaaaagaaa aaggattttt tgccagaaga aaagataagt
    22081 catttttaga agtaccatgg agaaatgtaa aaaaaatagg taatgacatg ataattatag
    22141 aatatgaggg atatatttag gaggaaaaaa tgagtatttt agtgcaaaag tttggtggga
    22201 catcagtaga atcatatgaa aaaatgaatg aagtatgtaa gatagtaaaa gcatacaaaa
    22261 aaaatgatga agaattacaa ttagtactag tagtttcagc aatgggaaga aaaggagctc
    22321 catacgctac agatacactt ataaatttat gtagtgcagt aaatgatgag ccaagcaaaa
    22381 gagaacttga tttaataatg tcttgtgggg aaataatatc aggaacaatt cttgctaatt
    22441 tattgaatgc acagggtata gattctgttt tctttactgg tcagcaagca ggtataatta
    22501 caagtgatga atattctaat gcaaagataa agtatataaa tcctaaaaag ataaaaagag
    22561 ctttagatga tgggaaggtt gtagttatag ctggattcca gggggtaaca gatgatggag
    22621 aaataactac ccttggaaga ggaggaagcg atacatcagc agttgctatt ggtaaagctt
    22681 tagaatgtga aacagttgaa atttacactg atgtggatgg tataatgact gcagacccta
    22741 gggtggaacc aaacgctaaa gtattgagtt ttatagatta tgaggaagta ttccaaatgg
    22801 ctgacaaagg tgcaaaggta atacatccaa gagcagtgca attagctaaa agtggaaata
    22861 taactttggc tataaaaaat acaatgaacc caacttttga aggtacaaaa ataggttctt
    22921 tatgtagaca cttagaagat aatatagaat atgaacaaga aagagatttt aaagttgctg
    22981 ttgcaaataa agatagtgta gcacaagtta agattaagtc agcagaagaa gtgtttacag
    23041 aagtattaaa tgagatagaa aagaaattta taactataga tatgataaat ttcttcatat
    23101 cagaaaaagc atttgttgta gaagatgctg atataaaatc attgaaggaa atactagata
    23161 aatttgaatt ggattatgaa gtaaatagag actgtgctaa ggtaacattg atatgttcaa
    23221 gaattgctga tgaaatgtct ggtatcatgt caaaggtagt tagaggatta tcaaaagcag
    23281 gggtatctct tttacaaaca tcagactcaa atatgactat atcttgttta gttagtgaag
    23341 aagatatgca tacagcagta catgctatac accaacaatt ttatttaaaa taaacaatat
    23401 aatacaatta ttttttaatc aaatttgata atttcatata tatcttaata tatttgattt
    23461 atttggtaaa actaaattaa atatgatttt gataggagtt ttttttatga aagattatag
    23521 agaagataat gaaagatttg ataaagataa ttgtagtgaa gaatctagta gtgaagagag
    23581 agaatgtata aagcaatttg gtaccaatga aatgccacct caacctccta aggatattca
    23641 atgtataact ataattggag aaattgaggg acattttata ggaaatcctc aaaaaaaagc
    23701 tacaaagtat gaacatataa ttccaatgct gtactcaatt gaggaaagta atgatgttaa
    23761 aggagtatta gttgttttaa acactgttgg aggagatatt gaagcaggtt tagctattgc
    23821 agaactttta aacagtacat ctaaaaaagt agttactctt gttttaggag gaagtcatag
    23881 tataggagtt cctcttgcta cagctggaga ttactcattt atagcaccaa cagcaaccat
    23941 gattatacat ccagtgagaa ctactggtct tgtaattggc ataaatgaga cttttgaata
    24001 ttttaaaaag atgcaagata gaataattca atttataata agaacatcaa atatcaaaaa
    24061 agatgtactt gaaaaactta tgcatgaaaa agatgaactt gtaagtgatg ttggtagtgt
    24121 attaatagga aaagaagctg tggattatgg tcttatagat gaagttggag gactaaaaga
    24181 agcacttaaa aaattaagag agcttataaa agaatctgag gaagaaaaaa ataatgatta
    24241 aaatttgttt aaataataaa atttatgata taatgatgta ataaagtagg agtgttagtg
    24301 ttaaggcatt agcacttttt gctgtgaagg ggtaaaactt gctaaaaata aggagtgata
    24361 ttaatggcca cgaaaaagaa gaaaaaaaag aaggtaaata ccttaagttt taatgcagaa
    24421 tatcataatt taataacaat atttataggt gtatttttat tatatagttt aaattctaat
    24481 tctatgggat ggatacctgt gttaatgcaa aatcttttta agggactatt tggagggctt
    24541 tcaatagcaa taccatttat agtaattata acaggacttt taggattttt tgatggaaat
    24601 gaatatatat ataggctaag aaagactaaa ttatactaca taattatttt atttatattt
    24661 gtattttatg gattattaaa tgcaggtact cttcctgtag atagcccact aaaaggtaac
    24721 atgtttgatg atgttatgaa attgggtgta agtggacaag gtagtggctt gatagcaaca
    24781 actattgcgt attatatgag taaaatattt ggtatagtag gtggatggtt aataagtatc
    24841 tttgcactta tactttctgt aatgtttata tttaatattt ctataaaaga tatgatatca
    24901 aatgcaaaat caaaatctaa agcatcaaaa gacagcaatt taacgtttaa agataaaatt
    24961 gcaaatatga aaaaatctgc aatagatatg atgactgatg aagtagatga tactactata
    25021 aataaaaaac caggtttctt taaagggtta atgtcaaaag gtcgtaatac tgatgaagaa
    25081 gatgaatatt ttgaagaaga aaatagtgat ggtgtagatg ataagactat aaaaatagtt
    25141 ggatttaata aggcagaaga tgagtattta gagatattag aaggaactca aagtatgcca
    25201 gagttagatg ttttaaaaga attacaaaaa gctacaaatg aaaatccagt tatagacact
    25261 aaaccagaaa aaaaagttga tatagctaag cctaacttaa atatagaaaa aactcaacct
    25321 atgagtattg tagcagagcc agttaatgag gattattcaa actataagaa gcctagcatt
    25381 gagctcttaa ataaagtaaa taagaagtct gatgaaaatg gtaaaaagaa agtgttaaaa
    25441 aatgcttctc ttttagaaaa gacactatca gattttggag tggaagcaaa aattaatcaa
    25501 gttacagttg gacctactat aacaaggtat gaaatacaac caagtccagg agtcaaggta
    25561 agtaaaatcg taaatttaac tgatgatata gctttaagtt tagcagctaa aagtataaga
    25621 attgaagcac caattcctgg taaaagtgca attggaatag aagttccaaa tgaagaagct
    25681 caaatggttg gtgtgagaga agttctagaa agtgaagaat ttaataattt taactctcct
    25741 cttgcaatgg gacttggaaa agatgtagca ggaaaaatta taattggaga tataggtaag
    25801 atgccacact tactaatagc tggttctaca ggttcaggaa agagtgtatg tgtaaataca
    25861 ttaataagta gtattttata taaggcaaat cctgatgaag taaaattact attaatagac
    25921 ccaaaagtag ttgaacttgc aaattataat gggatacctc atctgttgat accagtagtt
    25981 actgacccta aaaaagctgc aaatgcttta aactgggcag ttacagagat gaatagaagg
    26041 tacaagctat ttgcagatgc tcaagtaaaa gatgtaacaa gttataatga gaaagcagaa
    26101 gaaaaacttc ctaaaatagt aataataata gatgagttag cagatttaat gatggcaagt
    26161 gcaaatgatg tagaagatta tatttgtaga ctagctcaaa tggcaagagc tgctggaatg
    26221 cacttgattg tagcaactca aagaccatct gttgatgtta taacaggggt aataaaggct
    26281 aatataccat ctagaatagc atttgcagta tcatctcaaa ctgattcaag aacaatcctt
    26341 gatatgggtg gtgcagagaa gcttctaggt aaaggtgata tgttattcta cccacttggt
    26401 gcagcaaagc cagttagact tcaaggtgca ttcatatcag aaagtgagtc ggaaaaagta
    26461 attgattttg tgaaaagtca ggttaaagat ggaataaaat atgaagaaga tataattgaa
    26521 actatatcta aggttaatac ttcaaagggt agcgatgagg atgagttttt gagtgaagcg
    26581 atagaatttg ttgtggaaag tggtcaagca tctgcctcta tgttacagag aagatttaaa
    26641 ataggattta atagagcagc tagacttata gactctatgg aagaaagagg aataattgga
    26701 gcaagtgagg gaagtaaacc gagaaaggta ttaataagca aacaggattt gcagaattta
    26761 gaaggtgaat agaattgtca gttataaaaa tagaaaaagc acttaagcta gacaaagtaa
    26821 tatttgtaga tgtaagaact gaaggggaat atgaagaaga ccatatattg aatgcaatta
    26881 atatgcctct ttttaaaaat aatgaacata atgaagttgg aacaatttat aaaatgcagg
    26941 gaaaacatga agcaatacaa aaggggtttg actatgtatc ttataaatta aaggatatat
    27001 atttacaagc agcagaactt gctttaaatt atgataatat tgtcatatat tgtgcaagag
    27061 gtggaatgag aagtggaagt atagtaaatt tattatcatc attaggtgta aatgtatatc
    27121 aacttgaagg tggatataag gcttatagaa attttgtttt agagtattta aggcatgtaa
    27181 tggatacaaa aaactttgta tctgttcatg gacttactgg tacaggaaag actgatttac
    27241 tacatttatt agatgaaaaa ggaatagatg tactagattt agaaggcata gctaaaaaca
    27301 gtggttctac ctttggtttt ataacgtttg ataaaaaacc accatctcaa aagaactttg
    27361 aaacaaaatt atttgaaagt atatttttct caaaagagaa ttatatcttt gtcgagagtg
    27421 aaagtaaacg agttggaagt gttacaattc caaatgaaat tttcgaggca atgacacgag
    27481 aaggacatca tatattatta gaatgtgata ttgaaaatag agttgataga ctttgtagag
    27541 attatattta tgataaagat gaaggtaatc tattggtttt aaaagaatgt ataaacaaat
    27601 ttagaaaaag attgggtcat aaagtagtag atgattatat tgatttatta gaatcaggtg
    27661 aatataaaga actcgtaaaa agatatcttt tagaatacta cgacccactt tatatgcatt
    27721 cagtagagaa atataagtat aataaggtta taaactttaa tgatacagaa gaagctttag
    27781 aagatgttat agacttatat gagagtatat tggaaggaga aaatgaatgt taaaaatagc
    27841 attagaatca ttaggatgct caaaaaattt agtagatgca gaaataatga tgggaatttt
    27901 aaataacaaa ggctataaat taattggaga ttttgaagaa gctgatgtaa tcatagtaaa
    27961 tacatgtgga tttatagagt cagctaaaca agaatctata gatacgataa taaactttgc
    28021 cgaattaaaa aaaactggca atttaaagtt gttgatagta acagggtgtt tagcccaaag
    28081 atattctgaa gaattaaaaa ctgaaattcc tgaaatagat gctatagtag gtacaggaag
    28141 ctatcaaaat atagataaaa tacttagaga attaagtgaa atgcatcaaa ttgtaagcct
    28201 taatgatata gagtttgtat ttaatgaaga tttaccaaga tatctatcta cacctagcta
    28261 tatggcatat ttaaaaattg gagaaggatg ctccaataat tgtacttatt gtataatacc
    28321 aaaactaaga ggtaaatata gaagtagaaa atttgaagat ataattaaag aagctaagaa
    28381 attagctgaa tctggagtaa aagaacttgt agttatagca caagatacga caaagtatgg
    28441 ttttgattta tatggaaaag aaagattatc agaactttta gaagaacttg caaagataga
    28501 tggatttaag tggataagag ttatgtactc atatccagaa tctataacag aagaattaat
    28561 acaagttata aagaaatatg ataatatatg tagttacttt gatatgccaa tacaacatgc
    28621 aagtaacaat attttaaaat taatgaatag aaaaactact aaagaagata tattaaataa
    28681 aatcaatctt ataagaagta atataccaga tgctatactt agaactacta taatagtagg
    28741 atttcctggc gaaacagaag atgactttaa acaacttgtg gattttgttg aagaggttaa
    28801 atttgataga cttggtgcat ttgcatattc aagagaagaa gatacaccag cagatagatt
    28861 gccaaaccat atagatgaag acgtaaaaat acaaagaaga gatactttaa tgatgattca
    28921 acaaaaaata tctgaagaac ttaatgacaa aaagatagga aaaacttatg aggtgttaat
    28981 agaagaacag atagaagata atgtatatac aggaagaaca caaggtgatg cggaagaaat
    29041 tgacagtata gtgtatgtga aaagtgtaga caatcttgaa ataggagaat ttgtaagtgt
    29101 taaaataaat gatgcaatgg aatacgattt aatgggagat gttttatatg aacttgccta
    29161 acaagttaac tttgtttaga atatttttaa taccagtatt tgtcttaatt atgttgctca
    29221 atataccaaa taaattttta atagcttgta ttatttttat aatagcttca attactgatg
    29281 ctttagatgg aaaaatagct agaaaataca atctagtcac tgactttgga aaatttatgg
    29341 atccattagc agataagcta ttagttattt cagcacttac atgtatgata gaagaccatc
    29401 ttgtaagcag ttggatggta attataatag tggctagaga gcttacagtt agcatattaa
    29461 gagctatagc tgctgctgat ggaaaagtaa ttgcagctgg aaacagtgga aaacttaaaa
    29521 cgataactca aatggtatct atagtgcttt tattgttagg tgctcagttt gcaaatggat
    29581 tgatattaaa tattggtgaa attctcattc ttatagctac tctacttaca ttatattctg
    29641 gatgggaata tttatataaa aataaagagc tctttatgtc ttctaagtaa aaaattatgc
    29701 tttaataaat aaaaattaac aacttaaaat tttaaaaatg taaaaaaggg tatactatta
    29761 tagtatatct cttttcattt taagaatatt ttcttttgtg tatataatta ttaataatgg
    29821 tttataaata aaatgtaatg aataaaaata taatcattag tagcgttttt aaattgacta
    29881 ggtttgattt atgttatata attaatatat aacaagagaa caaatgtttg tagataaatg
    29941 aagggatggt atagagatga gtgtagatca agaaaaatta aaagcgttga atgaagcttt
    30001 aggtaaaatt gaaaaagatt ttggtaaagg ttcagtaatg aaattgggag aagcaacatc
    30061 tatgtctata gatgttatat caacaggagc gattggttta gatatagcta ttggtatagg
    30121 aggtctacct agagggagaa tagttgaagt atatggtcca gaatcttctg gtaagactac
    30181 tgttgcactt agttgtgtag catcagctca aaaagatgga ggaatagctg catttataga
    30241 tgcagaacat gcacttgacc cagtatacgc aaaagctttg ggcgtggatg ttgataacct
    30301 aataatatct caaccagata caggtgaaca ggctttagag atagcagagg cattgataag
    30361 aagtggagcg atagatataa tagtaataga ctcagtagca gcattagttc caaaggctga
    30421 aatagatgga gatatgggtg actctcacgt agggttacaa gctagactta tgtcgcaagc
    30481 tcttagaaag ttaactggtt caattaaaaa atcaaattgt gttgctatat ttataaacca
    30541 gttaagagag aaagtaggaa taatgtttgg aaacccagaa actactactg gaggacgtgc
    30601 attaaaattc tattcatcag ttagattgga tgttagaaaa atagacacaa taaaacaagg
    30661 agataaagtt ataggtagta gaactagagt taaagtcgtt aaaaataaag tagcacctcc
    30721 atttaagcaa gctgaatttg atataatgta tggagaaggg atttcaaaaa ttggagacct
    30781 tctagatata gctgctgatg tagatatagt aaaaaaatca ggttcatggt atagttacaa
    30841 tgatactaaa cttggacaag gaagagaaaa tgttaaaaaa ttcttggagg ataatttaga
    30901 tttaactaat gaaatagatg agaaagttag agcattttac aatttaaatg aagaacatga
    30961 agaagcaggt aattcagtat caaaagaaat tgtagaagaa taagtaaaaa tacaaaaaaa
    31021 taactagtaa ttaaagacat gccacgctcc ctattagctt gacacaatgt tgaaaaaatt
    31081 ataaaattaa gtgaggataa aatatctaaa tttaatttta attattagga atatttttta
    31141 atcatagtga tgacattttg ggattattaa cgaaggaggt ggatgtcata gatagtgtag
    31201 taatgattgt aataggtgca gctgttggta ttatagctgg atattttgtt agaaaaaata
    31261 tatctgaagc aaaaattgga caagctgaaa acttggccaa agaaataata gataaagcac
    31321 accacgactc agaaacagta caaaaagaaa agttactaga agctaaagaa gagatccata
    31381 agtggagaac agaagctgaa agagaaaaca gagaaagaag aacagaagta caaaagtatg
    31441 aaaaaagagt agtacaaaaa gaagaagtat tagatagaaa attacaaaat ttagaatcta
    31501 aagaagtaaa cctaagtgaa aaattaaagg tagtggaaaa gaaagaagaa gaagttgaag
    31561 taataaaaac tcaacagcta gaaaaattag aaagtatatc aggaattact tctgataagg
    31621 caaaagaaat catcttaacc aatgcagaga gagatgtaag acgtgaaatg tctataatga
    31681 taaaagagat agaaagtcaa gctaaagaag aagctgataa gaaatcaaga gaaattatag
    31741 gatatgcaat tcaaaaatgt gcagcagatc acgtagccga aactactgtc acagtagtaa
    31801 gcttgcctaa tgatgaaatg aaaggtagaa taataggtag agaaggtaga aatataagga
    31861 ctttagaaac cttaacaggt atagacctta taatagatga cactccagaa gccgtaattt
    31921 tatcaggatt tgatccaata agaagagaaa ttgcaagaat tgctctagaa aaattaatag
    31981 cagatggaag aatacatcca gctagaattg aagaaatggt tgaaaaggcg agaaaagaag
    32041 ttgacaatat tataaaagaa tatggtgaac aagctgcatt tgaaactagt gtacatggaa
    32101 ttcatccgga attaataaga ttattaggta gacttaacta tagaactagt tatggacaaa
    32161 atgtacttaa acattctata gaagttgctc atatagctgg tataatggct gctgaaatag
    32221 gagcagatat tagattagct aagagagcag gtttattgca tgatataggt aaggctgtag
    32281 accatgaaat ggaaggtaca catgttgaaa taggtatgga tttacttaga cgttataagg
    32341 aatctaaaga agttatacat gccatgagta cccatcatgg agattatgaa cctcaaacag
    32401 tagaagctgt attagttaca gcagcagatg caatatcagc tgctagacct ggagctagaa
    32461 gagaaacttt agaagcatat ataaagagac ttgaaaaatt agaagaaata gctaattcat
    32521 atgaaggtgt ggaaaaatca tttgctatac aagctggtag agaaataaga ataatggtaa
    32581 aaccagaagc tataaatgat gaagaaatac acctattggc tagagacatg actaagaaaa
    32641 tagaggatga acttgaatat cctggacaaa taaaagtaag cataataaga gaaacaagag
    32701 ctatagaata tgcgaagtaa aagaaataat atctaaataa taagaaaata tttttagaaa
    32761 tttaaatttg ccacaagata taatatatct tgtggttttt aaattgttta aatttaattt
    32821 tatataatgt aaaataatct aagtgctaaa agtgttactt acaataaatt aatgaaataa
    32881 agatttatat atgtattgtt tatataatta tccaaatttg tttgatataa tgaagttgta
    32941 aaataaatat attactactt ggaatgctta tacttaaata atatgaaaat acaataaagc
    33001 taaagtgagg tttaaagaga gggtacacta tgaaaaaaaa tacactttta catacaaata
    33061 ttatggtttg cattattatt actcttggtt ttgtggctac ctctataatt ggataccaat
    33121 ctaataccaa tatttttaaa aatgatattg aacatgtgtc tacattggct gctgaaggta
    33181 tttattatca gatagataaa ctactttcag aacctataaa tgtatctctt acaatggcaa
    33241 atgatagttt attaaaaaat tttttagatg gtgagaaaga acatttaaat gatgaagaat
    33301 ttatttataa attacaagat tatctgaatg tatatagaaa taaatactta tatgattctg
    33361 tttttttagt atcaactaaa actaataatt attatcattt tgatggactt aatagaaatc
    33421 ttagtgtcaa taattcagag aatcaatggt attatacttt cttaaataaa gaggatgaat
    33481 actcattaaa tgttgataat gatgaagcaa gtgacaatag tattacagta tttgtaaatt
    33541 gtaagataaa agatgataat ggagcaacta tgggaattat aggtgttggg ttaaaggtta
    33601 attcattaca aatgttgctt aaaggatatg atgacaaatt tgatgttgtc actcacttaa
    33661 ttgatgataa agggtttgta caattagcag tagataaaac tggtcatgag catataaatt
    33721 tttttgaaaa ttccagtagt gacttgtcta attcaaaaaa attaatatta aacaatgaaa
    33781 aggaacaaaa aagtttttgg tattcatctg aaaaatcaaa acgttatatt gtatgccagt
    33841 acatacctag tttaaaatgg tatcttgttt tagaaaatga ttttacttta atgagtaaac
    33901 agctttatat gcaattttta aaagacattg gagttatagt tttaattata atatttgtac
    33961 tttttactat tacacatgtg cttaaaaaat acaaaaaaca aatagtgaag cttagtgttt
    34021 cacagaaatt agaatatcaa aagttgttaa gccaatctac tgaaggactt tatgaaaata
    34081 tttttgaaat tgatattacc aataatagag ctggttgtga agatgagaat agatattttc
    34141 atgaatttgg attagggctt aacactccat atgatgaagc actgcacaca atagctaaaa
    34201 accaaatcaa agaagaatat gcacaaggtt atttagatac attcttacct gaaaatgtga
    34261 ttaaatgtta caatagtgga attaataata tcagttatga ctttttaatt aaaaaagatg
    34321 aagaaagcta tcattggatt agaattaatg cacgaatttt cttttggaca tcagatgaat
    34381 ctgttcgtat gatatcttat cgtaaaaata tagatgaaga aaaaaataga gaattactac
    34441 ttctcgaacg ttcacaacgt gattctctta cagggcttta caataaagct acaacagagg
    34501 aatttattcg ttcattttta aaaaatgaaa gtaatcaaga cttagaacac gtttttctaa
    34561 tattcgatat tgatgatttt aaaaatgtaa atgataaata tggtcatgta tttggagact
    34621 ttgttataag tgaatttgct ttagaattaa aatctcaatt tcgtgaggac gatattgtag
    34681 gacgtattgg tggagatgag tttgtagtat taataaaaga ctttaatagt aataactatg
    34741 tacttataga taaattagaa cgtttttgct ctaagattaa tcagaaaaaa tttgctgagc
    34801 aagcaggatt ttctattgga tgtagtattg gtgtatcaat gtatcctgaa catggatatg
    34861 tatactctga actatatgag aaagcagacc aagcacttta ctatgcaaaa agccatggaa
    34921 aaaattctta tcatgtattt tcagaggatt ctactgatag tatttcattt catatagacc
    34981 ctagagacat aaaagtatta ttagaaatgg ctactgatgg tgttgtcaaa tttgcttgta
    35041 tggaaaactt aaaactactt tattttaacc aaaaaatgct tgaatttact ggatattcag
    35101 ctcaaactgt tacttctaca gattttaata ttttttcgcc tgtactacaa gatgacctac
    35161 aaatggtact cgatacatta aaagaagcaa tctctaaaag ggagactttt gaggtagttt
    35221 ttcgtttttg tcatacagat ggtcatattt ttcctgtaag gatgcaaggg aagtttgtaa
    35281 gtgaattata tcaaaatcgt tatcctgttt tttatgctat ttatacagat atatcaaata
    35341 tcaatacttt attagaaaat aaatagtaaa tgctacagta aaatctaaaa ataggatata
    35401 ataatattaa tataagaaga ttataagtaa aacctgctga taaaatagat tattttaggc
    35461 aggtttttaa attttttata gtaaatctta tttttagtca atagggttta ttttttaaga
    35521 actttattga ttaaaaaata gctacgcaca cttgtgactt tagtcatgag ttagtagcta
    35581 aattagtcag catataggg
//
LOCUS       DAMJVM010000033        34805 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.033, whole genome shotgun sequence.
ACCESSION   DAMJVM010000033 DAMJVM010000000
VERSION     DAMJVM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.033"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            58..795
                     /gene="cas6"
                     /locus_tag="PC523_001689"
     CDS             58..795
                     /gene="cas6"
                     /locus_tag="PC523_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF2475217.1"
                     /translation="MSRISVFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKEREFYYINAYAGTFFLKGD
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            800..2266
                     /locus_tag="PC523_001690"
     CDS             800..2266
                     /locus_tag="PC523_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475218.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKSDYKLKFT
                     CELNRNYLSLESAENIDPKYISDYILENQLFKVFKNGKKEALEKIFNNIDNLTYVNFI
                     EELDKSSATAKQKEDIKKDFKNRRFPYVRNSGKYGLNTSLENMEINVKNIVDTVIKSN
                     IEIDKIDLEQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKRSGQ
                     VESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKSFYK
                     KLLHEKISSIRLYRFDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREPGNT
                     KNLGKKMIDNGNLSNLKELLLDNISFLKPSNGTSREMDFVSSSYNIGLYIKLCWIVDG
                     GDEYKMKNYTESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQTIMH
                     VLVSNGILIGDGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            2285..3298
                     /locus_tag="PC523_001691"
     CDS             2285..3298
                     /locus_tag="PC523_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DevR family CRISPR-associated autoregulator"
                     /protein_id="HDF2475219.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIIFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNPYIGSTQLITNRGFLSSEFGRKYYDEKEENEVLR
                     DENFPTTQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            3314..4111
                     /gene="cas5"
                     /locus_tag="PC523_001692"
     CDS             3314..4111
                     /gene="cas5"
                     /locus_tag="PC523_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HDF2475220.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLKGNYYDYY
                     KEHKAQNSILNYEVLKDVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPH
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            4138..6546
                     /locus_tag="PC523_001693"
     CDS             4138..6546
                     /locus_tag="PC523_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF2475221.1"
                     /translation="MENIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSTSKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINDILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIED
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILDEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLNTRNSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIAFIESNKEENICEGKDVKVS
                     FIKEKEIKSIVEGALENKQKILIIKNTIDSVNRTYEFLKENLSDKYSGVDIDVLHSRF
                     KFKDKKEKYSKILNGKGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKSILNNVIKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEDNFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYRLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            6985..7932
                     /gene="prmA"
                     /locus_tag="PC523_001694"
     CDS             6985..7932
                     /gene="prmA"
                     /locus_tag="PC523_001694"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDF2475222.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQDGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKN"
     gene            7951..8709
                     /locus_tag="PC523_001695"
     CDS             7951..8709
                     /locus_tag="PC523_001695"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDF2475223.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRYKPGEKLEICD
                     KNNNEYICEIVNIDKSIVNLNILEKVDINRESELKIRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVAKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLTFKEAL
                     EDMKKNNINICPYENERTVPMKHALQNCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            8711..10009
                     /gene="mtaB"
                     /locus_tag="PC523_001696"
     CDS             8711..10009
                     /gene="mtaB"
                     /locus_tag="PC523_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HDF2475224.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INKIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRKLDVLFEQNLEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            10052..10402
                     /locus_tag="PC523_001697"
     CDS             10052..10402
                     /locus_tag="PC523_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HDF2475225.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDITDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            10565..10744
                     /locus_tag="PC523_001698"
     CDS             10565..10744
                     /locus_tag="PC523_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDF2475226.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            10774..11217
                     /locus_tag="PC523_001699"
     CDS             10774..11217
                     /locus_tag="PC523_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDF2475227.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            11328..11825
                     /locus_tag="PC523_001700"
     CDS             11328..11825
                     /locus_tag="PC523_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDF2475228.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLVYLMWLVYAGFLNYFI
                     WFINK"
     gene            12018..12575
                     /gene="raiA"
                     /locus_tag="PC523_001701"
     CDS             12018..12575
                     /gene="raiA"
                     /locus_tag="PC523_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDF2475229.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENVDFIDNSSEFDDYDDEEYENIVIERRKKFNVKPMSSEEAILQMELVGHNFYMF
                     RNQDNFEINIVYKRKAGGYGLIEQD"
     gene            12789..13025
                     /locus_tag="PC523_001702"
     CDS             12789..13025
                     /locus_tag="PC523_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HDF2475230.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            13038..14363
                     /gene="yqfD"
                     /locus_tag="PC523_001703"
     CDS             13038..14363
                     /gene="yqfD"
                     /locus_tag="PC523_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HDF2475231.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRSELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEIPSNPEKRPLNDIRNEFDEDNKDNKEQNNSDENNSNQNSNSNNN"
     gene            14401..15417
                     /locus_tag="PC523_001704"
     CDS             14401..15417
                     /locus_tag="PC523_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDF2475232.1"
                     /translation="MIIQKKFTIADGNFERELFGNFDENVKLIEKTLNIDVILREGNI
                     ILIGEEKNVDSALKLMNELHQTVSNGKHLDKQSISYSLSLLLEGSEQKIKELEGTIVI
                     TQRGKAVQPKTLGQKEYIKLIENNDITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRII
                     LTRPAVEAGESLGFLPGDLKDKVDPYLRPLYDALFEMLGADKFNKYLERGTIEVAPLA
                     FMRGRTLDNSFIILDEAQNTTSEQMKMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLI
                     QATEILKGVPGIGSIMLTDRDVVRHELVQRIIRAYEKHDKREEFKKEERKKAKMQEKT
                     FKRK"
     gene            15474..15935
                     /gene="ybeY"
                     /locus_tag="PC523_001705"
     CDS             15474..15935
                     /gene="ybeY"
                     /locus_tag="PC523_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDF2475233.1"
                     /translation="MDLILDDRQDKLEVSEELLEKIKDIILECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            15941..16660
                     /locus_tag="PC523_001706"
     CDS             15941..16660
                     /locus_tag="PC523_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDF2475234.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            16779..17180
                     /locus_tag="PC523_001707"
     CDS             16779..17180
                     /locus_tag="PC523_001707"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDF2475235.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSSFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHDIKDLLPNYFSGNDLK"
     gene            17245..18138
                     /gene="era"
                     /locus_tag="PC523_001708"
     CDS             17245..18138
                     /gene="era"
                     /locus_tag="PC523_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDF2475236.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            18150..19529
                     /gene="mgtE"
                     /locus_tag="PC523_001709"
     CDS             18150..19529
                     /gene="mgtE"
                     /locus_tag="PC523_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDF2475237.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEQREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIVARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLKEQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            19602..19757
                     /locus_tag="PC523_001710"
     CDS             19602..19757
                     /locus_tag="PC523_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HDF2475238.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            19723..20496
                     /gene="recO"
                     /locus_tag="PC523_001711"
     CDS             19723..20496
                     /gene="recO"
                     /locus_tag="PC523_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDF2475239.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTVSLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            20489..21088
                     /locus_tag="PC523_001712"
     CDS             20489..21088
                     /locus_tag="PC523_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HDF2475240.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            21248..22126
                     /gene="glyQ"
                     /locus_tag="PC523_001713"
     CDS             21248..22126
                     /gene="glyQ"
                     /locus_tag="PC523_001713"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDF2475241.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            22126..24192
                     /locus_tag="PC523_001714"
     CDS             22126..24192
                     /locus_tag="PC523_001714"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDF2475242.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQSDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKDEYIFATIKQDGKETSEVLKDILPDTIKSVVFPKAMRWGGKNLKFARPI
                     RWIVTLLNDKVLEFDLEGIVSSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIVGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFNREEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVRVKQ
                     LLKDKKYGVALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLEICDL
                     SKIVYK"
     gene            complement(24359..25906)
                     /locus_tag="PC523_001715"
     CDS             complement(24359..25906)
                     /locus_tag="PC523_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HDF2475243.1"
                     /translation="MDNFKKILLNEIPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSHHQLNKIYEMAIKHNLDKIHLTTRQAIQLHDLSIDSIVDI
                     MKEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEAFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEQYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLKKFKEYYKLEKENGG
                     LELNIEEIDYSKSGVKICIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLNTLLKELDN
                     IENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGVST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASTGNFYEWVNSNDDILNK
                     ITDKYKI"
     gene            26186..26953
                     /locus_tag="PC523_001716"
     CDS             26186..26953
                     /locus_tag="PC523_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDF2475244.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            27238..27435
                     /locus_tag="PC523_001717"
     CDS             27238..27435
                     /locus_tag="PC523_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2475245.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            27464..28540
                     /gene="vorB"
                     /locus_tag="PC523_001718"
     CDS             27464..28540
                     /gene="vorB"
                     /locus_tag="PC523_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2475246.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            28543..29262
                     /locus_tag="PC523_001719"
     CDS             28543..29262
                     /locus_tag="PC523_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2475247.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTVEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            29275..29814
                     /locus_tag="PC523_001720"
     CDS             29275..29814
                     /locus_tag="PC523_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2475248.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIRVPVTEIAQNLGNEKAANICMLGAMVKNLDLFTKEEFENGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            29971..31029
                     /gene="buk"
                     /locus_tag="PC523_001721"
     CDS             29971..31029
                     /gene="buk"
                     /locus_tag="PC523_001721"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2475249.1"
                     /translation="MKILVINPGGTSTKISVFKDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFEDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKQGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVIEKVSYIAPVEVVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            31047..31940
                     /locus_tag="PC523_001722"
     CDS             31047..31940
                     /locus_tag="PC523_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HDF2475250.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIENISKEIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMVNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPNCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            31970..33178
                     /locus_tag="PC523_001723"
     CDS             31970..33178
                     /locus_tag="PC523_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2475251.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEVTQKVLKERGKFALAYGPTKGEDELLELLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVSKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIREEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            33202..34461
                     /locus_tag="PC523_001724"
     CDS             33202..34461
                     /locus_tag="PC523_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2475252.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEKEEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
BASE COUNT        14032 a         3560 c         6437 g        10776 t
ORIGIN      
        1 ttttttatat tacaaataga gaataatgga ttatactaga aagtaggagg tgaaaatatg
       61 agtagaattt cagtttttat ggagtcatgt aatggcaaat ttccaaatga aaattcaatg
      121 ttgtcagttt cctttataaa aaatatttta aacgcagaaa acaaagattt tgctaaaaat
      181 atatttaact atggggaaga aagaaaaaac aataagcaaa taaaggatat aaatacagcc
      241 atctacatac caaatcttat aagaagcaaa aatgagttgc ttgttgatgg agatataata
      301 ttcaatataa gtttttataa ttattctatg ttcagtaaat tatacaatgg aattttaaaa
      361 gtaaaggagc tagaatataa aggttataga tttaaaataa aaaatataaa aatacataaa
      421 gaacatgaga taaaagaaaa tggtgttata tttaaaacta tgtctcctat aatagtaaag
      481 aataaagaag gtaaatattt agatgtagaa gattcaaatt atatagaatg tttaaactat
      541 atagctaatc taactctgga gagtataaga ggtagtgggc ttagaaagcc attggaattt
      601 atacctctta acttcaaaaa aagagtgtta aaagaaaaga ttcgtggatt taaagagaga
      661 gaattttact atataaatgc ttatgcagga acattttttt taaaaggaga tatggaagat
      721 ttaaatgcac tgtataaaat ggggattgga tatagaagga cggaaaatgc aggaatggtt
      781 gatatattaa agtagcgagg tgagagaaat aaaagctaaa ttttatagat caggtgatat
      841 tgtaagagat attggcattg tttactttta tgaattacta atggatttaa aaaacgaact
      901 agaaaaaagt gattataaat taaaatttac ttgtgagtta aatagaaatt atttatcttt
      961 agaatctgca gaaaatatag accctaagta tatatctgat tatatattag aaaatcaact
     1021 ttttaaggta tttaaaaatg ggaaaaagga agccttagaa aaaatattta ataatataga
     1081 taatttaacg tatgtaaatt ttattgaaga attggataaa tcttctgcaa ctgctaaaca
     1141 aaaggaagat attaaaaaag attttaaaaa cagaagattt ccatatgtta gaaatagtgg
     1201 taagtatgga ttaaacacga gtttagaaaa tatggaaata aatgtaaaaa acatagttga
     1261 tactgtgatt aaatcgaata tagaaataga taaaattgat ttagaacaat atgaagaaaa
     1321 agatagtgta tgtaatgttt gtaatataaa taaaacaaca aaactagata ttgatttaag
     1381 agagagaaag gactctaagt ataacttttt atttagaggt gcagaaaaat caggctttaa
     1441 aagaagtgga caagttgaaa gtaatatttg ttttgagtgt gaatttttca acttaatgtg
     1501 tcttttatat ataaatttaa aaagaccaat ggtttttgca tatacgaatg atttaaggga
     1561 attagcattt ttaaatcata agataatgct taaaagaaaa atgtattcag ataaatcatt
     1621 ttataaaaaa cttttacatg aaaaaataag ttcaattaga ctatataggt ttgatattga
     1681 tacgaataaa ggtataattc taaagtttga ttctattata gagtataaag aattactaaa
     1741 aaaaattgaa cttatagata ttatagataa ttacaatttc tcaagagagc cagggaacac
     1801 taaaaattta ggtaaaaaaa tgattgataa tgggaattta tcaaatttaa aagaattatt
     1861 acttgacaat ataagttttt taaagccatc aaatggaact tctcgagaaa tggattttgt
     1921 atctagttca tataatatcg gcttatatat aaaattgtgt tggattgtag atggagggga
     1981 tgagtataaa atgaaaaact atacagaaag taatttaata tattcaagag taggtaaaga
     2041 cttatttaat aaaatgacag atgaatccag aagaaaaaat ttttctatga aagtgattca
     2101 actacttaaa tctaatgata gaactcagtt atttcaaacc ataatgcatg tgttagtttc
     2161 taatggaatc ttaataggag atgggtttgt agaaggtcta atgcagagtt ctgaattaga
     2221 attaaataca aatgtaggtc tatttattca agaaataatg aaataaaaag ggggaaaatt
     2281 taaaatgaat gaaaaaagag taaaaggagt aacattaact atactgacag aaagcccagt
     2341 tccactttca tatgaccaag gttatggaaa ttatacacca ataaaaaaag aacaatatag
     2401 agaaaaaata catgcaaaaa ctagtatagc tacaataaca tatgacttaa gaagaatgct
     2461 acatcaagaa tatggttgga atttaagtaa tattattttt gggaaaaaag gaaatatata
     2521 tccatctatt aagaaaaatg taggatgtgt agatgaaaat ggattagaga ctgatgtatt
     2581 tggttatttg ataccactta aagataaagg ttctataagt aaagcttctc cacttagaat
     2641 aatacctttt aggtcattaa atccatatat aggttcaaca cagctaataa caaatagagg
     2701 ttttttaagt agcgaatttg gtagaaaata ttatgatgag aaggaagaaa atgaagtact
     2761 tagagatgaa aactttccaa caacacaagc attggcaata gaagagactt tatcagatta
     2821 ctacgtttat acaataacat tggagcttga tagaatagga gtagtagaag tagaagatgg
     2881 gaagttactt ttaccagaag aaagaaaatt tatgagtaag gaattaagag aaaaggcagt
     2941 aaaagatata ttagatgcaa ttactgtgtt tacaagaaac ataaaacatt catcagtact
     3001 attaaaacca cttgcagtaa tgggtggagc ttttgataaa gttgtaccgt ttttttggga
     3061 tgatgtagat tacaatgctg atagtggtga gataaattta gagggagtta tagaaactat
     3121 agaaagttat agccttaaag aaagtaatac aatacttgct attaatgaca gattaaaaat
     3181 ttctaataaa aaggaattaa ataagtttaa tttaagtaaa taccctgtaa aagagattaa
     3241 aaatctagca gacagattgg aaattggaga agataatatg tggtatttaa aagagtagga
     3301 ggttttttga attatgaaag tattacaatg taaacttaag caaccaactg ctcactatag
     3361 agacccaaaa gtatttcaaa atgaatatat aagtacttta aatttaccat ctaagactac
     3421 aataatgggg atgataacgt atttatgtga tagaagatta aactctgata tagatatagg
     3481 aataattgga acacatcatc atagagaact agaattttca agaggagaga atattgattt
     3541 ttggaatgaa tatacaaata tgagaaaagg taaagataaa gaaaagtttt tgcttaaagg
     3601 aaactactat gattactata aagaacataa agctcaaaat agcatattaa actatgaagt
     3661 tcttaaagac gtagaactga ctatatttat ttcttgcaag gatgatgaag aacttgaatt
     3721 tattagaaaa aagcttgaaa gtccatgtaa atatgcaaat ttaggaagaa aagaagattt
     3781 tgtaatacca agtgaaaaag ggtgttttgt gaaagaagtt gagttaaaag aggttatacc
     3841 tatgaatact cgtgatgcta taaaagaaaa tataaaatta aagaatacat atgtaagagt
     3901 tgacttaaga gataaagaga atgtcgagac tataataaat caaggtgtat tgattgcatt
     3961 gcctcataaa tataaagatt tagaagcaaa tagagacgat agagtttatg agttttgcca
     4021 ttatatatat gttgataatg atggtatata tcctaaaaat attaaggtaa atgtatgtat
     4081 tgatacaaaa gaagttttta catggcttta aattattttg aaagataggt gtttatcatg
     4141 gaaaatatca tgcaaaaaga aatattagct aagtcataca atttaagaac taatcaacaa
     4201 gagcaaacat tagttgaaca tattaaggat ttatttgaag tattagaatc tatattagaa
     4261 ctaaatctat attcagataa agatgtagag atattgaaaa tatgttgtgc tttgcacgat
     4321 ttaggaaaaa ttaattcgat tatgcaacaa aaaatggaga ttaataataa aatatcgtat
     4381 agttgttcag aagaagagag gaaaaagtta gagtctgaca aaaaatcttt aaaaaagata
     4441 gcaagacaca atatattttc tggagcattt ttaaaagata tattagaaaa aatgaacttg
     4501 tcagaagaag ataagttata tatatataaa agtataatgt tacatcatgg aaactatgaa
     4561 gattatatga ggttaagtac tagtaaagtt caagaagaaa tttatgagta tattgaaaaa
     4621 ggcattttag aaaaagaaga gtttaatctg aaagatatag aaagttatat aaatgacatt
     4681 ttaaatgtag attttaaatt tggtgaagat gtattggatt atgattacat agataaatta
     4741 agtgaaagta tgtttataga cagtgattat aacaatggtc aaatagaaga ttcagtttta
     4801 aattatagaa agtttaagta tattttatat aaagggatgt tgaatttaat agaccatagt
     4861 gcatcaagta gacagaaagg tataaaattt tataacgatt ttactgatga agaaattgat
     4921 aatatgattt tagatgagat atataagagt cagggtaatt tagaaaaaaa taatatagag
     4981 ttcaatacta tacaaaaaag gttgagagaa ctttctggaa gaaatgttct gactgttgca
     5041 tttacaggtt caggaaaaac cgctgcagat tatagaaaga cttttaaaag aaaattcttt
     5101 ttagtaccaa ataaaatatc agcagaaagt ttttatagaa aaaatatttt tcaaaataaa
     5161 aatgatttaa atacaagaaa tagtaatgat tatattggat tacttcatgg agatataaac
     5221 ttatactcag agaatgaaga taatggagaa catgattttg tattgacatt aagggatata
     5281 gatttgagta taaacttttg taaaccttgt gtaattgcaa cagttgacca gttattactt
     5341 agtatgttta aatttccggg atatgaaaaa atatttgcag ctgtaaagaa tgcatctata
     5401 actgttgatg aggtacattt attagaacca aagatgtttt tgattttgat atattttatg
     5461 cagtttgcat gcaagtattt agatgttgat tttcatttaa tgacagctac acttccaaaa
     5521 tcatataaag agcaaatgat aaacaaggga attatttttc aagaagaaag taatgaaaat
     5581 gttactgata aaggagaaat agcgtttatt gagagcaaca aggaagaaaa catttgtgaa
     5641 ggtaaagatg taaaggtttc ttttataaag gaaaaagaaa tcaagtcaat agttgaaggt
     5701 gctttagaaa ataaacaaaa gattcttata ataaagaata ctattgattc agtaaataga
     5761 acatatgagt ttttaaaaga aaatttatca gataagtata gtggtgtaga tattgatgtt
     5821 ttgcatagta gatttaaatt caaagataaa aaagagaaat atagtaaaat attaaatgga
     5881 aagggtgata tttggatatc aactcaatca gtagaaatat ccttggattt ggatttcaat
     5941 ataataataa gtgatttagc aactatggat agtttgatac aaagaatggg tagatgcaat
     6001 agaaataata aatatgaata tggaaacttt tatattctac ctagtgaaga taaaatttat
     6061 gataacaaac ttaagaacac aactaaaagc atcttaaata acgtaataaa aactaactct
     6121 atttttacaa tggggattag aaaaactatt ttggatgatt actatgataa tagtgtagtc
     6181 aaaaaatatt ttgaagataa ttttataagt tgtgataaag aaattaagaa tatttatggt
     6241 ataaataaag aaatttttga tggattggat ttaattttta attttgaacc ttataaaaat
     6301 attgttgata gtaagaagga agcagctaaa atttttagag atgttgatgt tagttacaaa
     6361 attattttgg aagaagattt ttataaggaa gatagggagt tacaacaaga ttcgattcaa
     6421 gtatcagggt ttatatttaa tagattatat tattatagat taataagcaa agtagaagga
     6481 tacatggtac taaaatctag tagtaagttt gagtataata gtactgtagg cttaatttta
     6541 aagtgagcat atgtttaaat ataatatgcc tttatacatt ggaaatacaa agagcactaa
     6601 tgtaagccca ctgtaaaaac tagtttttta aaagaaaaat aagttgtgca atagagtgtt
     6661 cacacgtgta tatatagcaa atttggggtt taagattaac taagtggatt tcaggtacat
     6721 catgaaaata attataacct ggagattttg tatgtttaat attaactaag tagatttcaa
     6781 atattgatat agtagatata atagaaattt tatgattagg tttaaaatta gctaatcgaa
     6841 ttttctaata ttctgactat tatataagta tattattatc ttgaatttaa aaccaataat
     6901 ataaaacaac aaaatattat atttatgata taataaaagt tatggaaaaa taggaagagt
     6961 actatagaga agagaggtaa acttatgaat aattggatag aagtaactat aaaaacaaca
     7021 acagaagcag ttgagcctat aacgaatata ctatatgaac aaggagctgg aggagctgta
     7081 atagaagacc caaaagattt tttattccaa aagaaaaatg aactagattg ggattatgtt
     7141 gaagaagaag tatttaaaaa gaatgaagaa gatgatgttc ttataaaaac ttacgtttct
     7201 gaagaaaaaa acgtgatgga atttgtcgaa ataataaagc aaaaagtttt aggcttaaaa
     7261 gattttggta tagatatagg tgaaggaagt gtatcattag accaagtaaa tgaagctgat
     7321 tgggcaaatg catggaaagc atactacaaa ccaacaaaag taggacaaag ggtagtagta
     7381 aaacctacat gggaagatta tgctatgcaa gatggggatt taatcataga gcttgaccct
     7441 ggaatggcat ttggtacagg tacacatgag actactagta tgtgcattag agaattagaa
     7501 aagtatgtta ataaagattc taaagtattt gatattggct gtggtagtgg aatccttgca
     7561 atagcagcag ccaaattagg agcaaaagaa gttgtagctg tagatttaga tgaagtggct
     7621 gtaaaagttg caaaagaaaa tgttcttgaa aataaagttg aaaaaagtgt atctgtaatg
     7681 catggtaatt taactgatgt aataaaagat aaagcggatg taatagttgc aaatataata
     7741 gctgatataa taaagatact tgcaaaagat gttcaaaatt ttatgaaaga agatgctata
     7801 tttatatctt caggtataat tttggataaa gtagaagaag taaaagaaag tttaattgaa
     7861 aatggatttg aaatagttga agttcaaaaa ttaggtgaat ggtcagcaat agtttctaag
     7921 cttaaaaatt aagggatatg gtgacgtata atggatagat tctttgttga aaaaaataat
     7981 ataaatctac aagataaaac ttgcactata gaaggtgaag atgtaaaaca tatttcaaaa
     8041 gttttaagat ataagccagg tgagaaactt gagatatgtg acaagaataa taatgagtat
     8101 atttgtgaga tagtgaatat agataagagt atagttaact taaatatttt agaaaaagtc
     8161 gatataaata gggaatcaga acttaaaata aggttatatc agggacttcc aaaagctcca
     8221 aaaatggaga tgatacttca aaaattaact gaagtaggtg tagaagaaat aatattagtt
     8281 caaactaaga gaagtgttgc taaagttgat gacaaaaaag aagataaaaa atttgaacgt
     8341 tgggaaagaa taatttatga agcagcaaag caaagtaaaa gaggtaagat accaaaatta
     8401 agaggtgttt taacttttaa agaagcttta gaagatatga aaaaaaataa tataaatatt
     8461 tgtccttatg aaaacgaaag aacagttcca atgaaacatg cactacaaaa ttgtgactct
     8521 aatatagata gtgttggaat ttttataggt ccagaaggtg gattttctga agaagaaata
     8581 gaacaaatac aaaagaataa ttgcaatgtt gtatcactag gacctagaat actaagaact
     8641 gagacagctt ctgttgtagc atcaacaata gctttgtatg agctaagtga tttaggagga
     8701 gaaaaataaa ttgaaaaaag tagcttttta tacacttgga tgtaaagtca atcaatatga
     8761 aacagaagct atgctagaac tatttgaaaa agatggctat gaacaagtca atagtgaaga
     8821 gtatgctgat gtttatgtta taaatacatg tacagttact catatgagtg atagaaaatc
     8881 tcgtcagtac ataagacgag ttaaaaagaa gaacccagac gcaataatag ctgttgtagg
     8941 atgctattct caggtatctc cagaagaaat tttggatata gaagaagtaa atttagtcat
     9001 gggtacaaat gatagaagaa agattgttga agaaattaaa aagataaatt caagtaagaa
     9061 agttagtaca gttgatgata taatgaaagt taaagctttt gaagaaatag aaatcagcca
     9121 aactaatgga aaaactagag catttatgaa aatacaagat ggatgtgaca gattttgtac
     9181 ttattgtatc attccttatg cccgaggacg cgttagaagt agagatattg atagtatagt
     9241 cgatgaagtg aaaaaattgg ctaataatgg ctataaagag gtcgtattaa caggaataca
     9301 tgttgcttct tatggaaaag atttaaagga tagggatatt aaacttcttg atgtaataaa
     9361 acaaataaac aagatagaaa aaatagaaag aattaggctt agttctgttg aaccaatttt
     9421 atttacagat gaatttgtaa atgaagtttt aaagatggat aaagtttgtc ctcattacca
     9481 tttgtcactt caaagtggat gtgatgaaac tttaaaaaga atgaatagaa gatacacaac
     9541 tctagagtac aaaactattg tagatagatt gagaagtaaa atgccagatg ttgcaataac
     9601 tacagatgta atagtaggtt tccctggaga gacaaatgaa gagtttaaaa aaacgtacga
     9661 gtttttaaaa gaaatagagc tttcacaaat gcatattttt aaatattcac caagaaaagg
     9721 aactccagct gcaactatgg aaaatcaagt tgaccctcaa atgaaacatt ttagaagcga
     9781 acaactttta aatttgagta aagtaaattt taataaattt gcaactaaat ttatcggccg
     9841 taagttggat gttttatttg aacaaaatct agaaggcaat aaatatgagg gattaacttc
     9901 aaattatata agagttgttg ttgaaagtga caaaaatata caaggtcaga tattaaaagt
     9961 gaaaataaat gatgtaaaag atgaatatgt agaaggaatt ttgctataac atgtagaaat
    10021 tataatattt ataagatagg aggtggaaag aatggattgt attttttgta aaatagcgaa
    10081 cggagaaata ccttcaacta aagtttatga agatgataga gtcttagcct ttaatgactt
    10141 gaacccagtt gctccatatc atatattagt tgtacctaaa aaacattatg atagtttaat
    10201 tgatattact gataaagaga tggacattgt gtcacatatt catgttgtta ttaataaaat
    10261 agctaaagag aaaggttttg accaaactgg attcagagtc ataaataatt gtggaagtga
    10321 tggagggcaa gaagttaaac atttgcacta tcatatttta gctggtaaaa agcttcctaa
    10381 ttacgaagcg ggacaaaact aaactaaatt attgaaaaat tgagttcctt cttgataaaa
    10441 atacttgata aaagtaaaaa tttagtttat aataaacaaa gtgttaggtt gtaatgcata
    10501 ttatgcaaaa agaacatagt tacagtccca gcataattgc gctattggag ggagggaaaa
    10561 agaaatgtca gaagtaagag ttagagagaa tgaaacatta gacagcgctt taagaagatt
    10621 caagcgtcaa tgtgcaatgt ctggcatcat gtcagaagtg agaaaaagag agcactatga
    10681 taagccaagc gttaagcgta agaaaaaagc tgaagcagca agaagaaaaa acgctaagaa
    10741 atagtagtaa ataagaataa agaggtgaag gggatgtccc ttaagcaaaa gttacaagag
    10801 gatttgaaat cttcgatgaa aaacaaagat acagtgagaa aatctgtagt aactttaata
    10861 agagcttcta taaagcagta tgaggttgat aatagggtcg agcttgatga agatggaatt
    10921 atcgatgtaa tagcaaaaca attaaagcaa cgtagagatg cattagttga atttgaaaaa
    10981 gctggaagag aagacttaat aaaagaaaca gaaggtgaaa ttgaagtttt aaaagagtac
    11041 ctacctcaac aattaagcga agaagagtta gaagaaattg taaaatctac tatatctgaa
    11101 gtaggggcaa cttctatgaa ggatatggga aaaataatgt cagttatcca accaaaagtt
    11161 aaaggaagag ctgacggaaa acttataaat aagttggtaa aacaaaactt acaatagatt
    11221 gatatagcct agagagaaat ctctaggctt tttataattt ttgggaataa cgagtcatat
    11281 ttgagcatat ctataatcaa agaaaatttt gaaagaaggg gaaaaatttg attatatcag
    11341 taaaagagat taggaatttt ttgataaaca tatttatacc actaataata ggttacttaa
    11401 gtagcatttt aacaatgatt atcagtggaa cagatatttc aacctattat ttacagctca
    11461 caaagccaag ctttgcacca ccagctttta tatttcctat agtttggact atattgtaca
    11521 tattgatggg tatatcttca tacctaatat taagaaaggg ttacaactta cctaaagtaa
    11581 gagatgcaat tttttattat ggattacaac ttgtattaaa ctttatttgg agtatactat
    11641 tttttggatt tggacttaga tttacagctt tgatagatat aataataatg attttggttt
    11701 ctataataat gatttcgaag ttttctaaaa tagataagag atctggatac ataaatttgg
    11761 tttatttaat gtggcttgtt tatgcaggat ttttgaacta ttttatatgg tttattaata
    11821 aatagaatgt aaagattttg taaattaccc tgtactttta ataatatcat aacaaacgaa
    11881 aaataaatag actattaaaa ataatcattt tcatatacaa taatattaat ttaaatataa
    11941 taaatttata aaaattgtat ttaaagatta acatattaaa gtattagaat tggaaaggct
    12001 tgaagggaga ggtttttatg caaataatag tatctgggag acaaatgaaa ttaactgacg
    12061 gaataaaagg atacgtagat ggaaaattaa gtaggttaga aaaatacctt gatcctgaat
    12121 cagaagtgaa agtaacagta agtgcgaaaa aggatagaca aaaagtggaa gttacaataa
    12181 ttcctatcaa tggtcaaata ataagagcag aagacgtaga agatgactta tatgcagcaa
    12241 ttgatattgt ttgtgacaaa ttaagtagac aagttgtcaa atataaaact aaagttaaag
    12301 ataaagttca aaataataag agcataagat ttgaaaatgt tgactttata gataatagca
    12361 gtgaatttga tgattatgat gatgaagaat atgaaaatat agttatagaa agaagaaaga
    12421 aatttaatgt aaaacctatg agttctgaag aggctatact tcaaatggaa ctagtaggac
    12481 acaatttcta tatgtttaga aatcaagata attttgaaat aaacatagtg tataaacgta
    12541 aagctggtgg atatggttta atagaacaag actaataaaa aattgtatac gtttagattt
    12601 tatataagaa aaaataaaga ggtaatcatg ttgatttttt atcaatatga ttaccttttt
    12661 attgttcaaa taatatatta gatattaggt gtttattata taaaaatttt aaaactaatc
    12721 tgttacaaca tcaataaagt gcataaatac ctatttcaag cataaaatta attaaggggg
    12781 taatgcttat gttagaaata tccactgatt tacaagtaaa tcaaccagtt attactgtta
    12841 caagtaatac gtttattagt atagaaaatt atctatcaat attagaatat gaagttgatt
    12901 taataagaat aaagactaag gttaaaacaa ttaaaatatc tggggataaa ctttcactta
    12961 aatatattac tgatagtgag ataggtataa aagggattat ctacaatgtt gagtatgttg
    13021 attaggagga gatacacttg ataagtttta taaggggata ctatgttata gttgtggaag
    13081 gagtaggact cgaacaattt ttaaatcatc ttataagaaa tggtataagt gtttacaatg
    13141 taactagaat aaaaaatact aaaatggagt ttcatataga tagacaagat ataaaagaat
    13201 ttaagaatgt ttatagaggt agtaagtttg atataaaggt aaagcaaaaa actggggttc
    13261 cttttattat caaaagagtt tataagcaca aaggtatgtg gatttgtgct cttgtatcgt
    13321 tatttttatt gatgtcgaca tcacagtttg tgacagatgt atatatacaa tctcctgaag
    13381 gtataaaaaa agaagctctt agaagtgagc tttataaggt tggagtaaga ccaggagtct
    13441 ataagaagag tattgataga aaagaagtta gagaccatat gatgtcgaaa tttaatgatg
    13501 tggcatattt atctatcaat gtgaaaggaa caaatatatt tgttactgtt acaaaaaagg
    13561 cagaatccct aaaatctaca gaccaatcaa attattgtaa tgtaattgct ttaaaaaatg
    13621 gtataataga aaaggtaatc cctagaagtg gtaaatcagt tattaaatct ggagatatag
    13681 tccaaaaggg agatgtcttg ttaaatggag ctaataccaa gtcaatacca gaagtatggg
    13741 caagtacttt ttatgagtcc acaaaaaagg caagttatgt agatactgta aataaaaaaa
    13801 ctggagaaaa gaaaaatata tatacactaa gtttttatga taaagagttt acaatgagaa
    13861 aaaatataaa atataaagat tatgttgttg aaaataaaga aaaaaaacta tcaataggaa
    13921 actatacttt tccaataaag ataaaaacga gcacttttta tgaaacaaaa aaggttgaag
    13981 taaaaaggaa taaagaagag ttaaaaaaag aattatctga aaaagcttta aaagagttag
    14041 aatacataat tccagcttcg gctagaataa tagatgtaaa acataacttt aaggttaata
    14101 aaaatatgtt agaatattta ataaccgttc aaactagtga aaatattgct aaaatttatc
    14161 ctcttagtaa atcagaagca gaaaggttta taaaggaaga aagtaaacca gatgaaggtg
    14221 aagaggaaat tccttccaat ccagaaaaaa gaccattaaa tgatataaga aatgagtttg
    14281 atgaggataa taaggataac aaagaacaaa acaattctga tgaaaataat agtaatcaaa
    14341 atagtaatag caataacaac taataaaatt aaacttaaaa tcttaggagg aataattcaa
    14401 ttgataatac aaaagaaatt tacaatagca gatggtaact ttgaaagaga gttatttggc
    14461 aactttgatg aaaatgtaaa attaatagag aagactttaa atatagatgt aatacttaga
    14521 gaaggtaata taattttaat tggagaagaa aaaaatgtag attctgcatt aaagcttatg
    14581 aatgaattac accagactgt atcaaatggt aagcatttag ataaacagag tatttcttat
    14641 tcattatctc ttttacttga aggtagtgaa caaaaaataa aagaacttga ggggacaatc
    14701 gttataactc aaagaggtaa agctgttcaa cctaaaactt taggtcaaaa agaatatata
    14761 aaacttatag aaaataatga tataactttt ggcgttggac ctgctggaac aggaaagact
    14821 tatcttgctg ttgctatggc agtaaaagct tttaaaagag atgaagtaag tagaattata
    14881 cttacaagac cagctgtgga agcaggagag agtctaggtt ttttaccagg agatttaaag
    14941 gacaaagttg acccatactt aagaccacta tatgatgcct tatttgaaat gctgggtgct
    15001 gataaattca ataagtatct agaaagaggt actattgagg ttgcaccact tgcatttatg
    15061 agaggtagaa cacttgataa ttcatttata atattagatg aagctcaaaa taccacttct
    15121 gaacaaatga aaatgttttt aactagacta ggatttggtt ctaaagctgt agttacaggt
    15181 gatgtaactc aaacagattt accacaaaat aagaagagtg gtctaataca agctacagag
    15241 attcttaaag gtgttccagg tattgggtct ataatgctaa cagatagaga tgttgtaaga
    15301 catgagctgg tacagagaat aataagagct tatgagaagc acgacaaaag agaagaattt
    15361 aaaaaagaag agcgtaaaaa agcgaaaatg caggaaaaga ctttcaaaag aaagtaaaag
    15421 tgtagtaata tagatatata gcattattaa tgaataaaaa gtaggtgtag tgaatggatt
    15481 tgatactaga tgatagacaa gataaattag aagtaagtga ggagttactt gaaaagataa
    15541 aggatataat cctagaatgt ttagattatg aaggatatga tgataattat gaagtgagtc
    15601 tttcttttgt agataataaa gagattcatg aattaaatag agaatataga ggagttgata
    15661 gagtaactga tgttttatca tttcctcttt taagtgatga ttttgaagat gttgagttag
    15721 aagaagaatc attaggcgat atagtagtat ctcttgaaag agccttagaa cagagtatag
    15781 agtataacca tagttttgaa agggaagtat gttttttaat atgtcatagt atgtttcatc
    15841 ttttaggata tgaccacgat acagatgaaa atacaaaaga aatgagagaa aaagaagaac
    15901 acatattaaa taagttaaac ataacaaggg agtagttttt atgaaaccag aaaaaactcg
    15961 tcaaggtata attaaagcgt ttaatgcagc tatagaaggg atattgtata catttaaatt
    16021 tgaaagaaat atgaaaatac attatttagg ttcagttgct gttcttataa taagtttgtt
    16081 ttttaacttt tcaaaattag aaatgataat gttgcttatg tccatatgtt tagttgtagt
    16141 tgcggagatg tttaatacag ctatagaaaa agctgttgat ttagtaactg atgaatatca
    16201 tgttcttgct aagatagcaa aagatgttgc agctggaggt gttctagtag cagcactaaa
    16261 ttcagtagta gttggatata ttttgtttta tgataaattg acagatatat ctgggatatt
    16321 aatttataaa ataagagaat cagaattaca tataaccttg atatgtatat tattggttct
    16381 tatagctgtt gtagttgtaa aagcattgac ttctacaggt actcccttaa aaggaggaat
    16441 gccaagtggt catgctgcat tagcatttgc tatagcaact gcaattacac ttatgactga
    16501 aagggttgtt gcatctactc ttgcatatat aatggctgtg ttagttgccc aaagtagaat
    16561 tgaaggaaaa atacatacct tttgggagac tatcgctgga gctttattag gtgtgttgat
    16621 agctatatta gtatttcagc ttggaatgtt ctataattaa aatgaatgta cataaaatat
    16681 ttaacataaa acaaatctgt gctaattaaa tttttatgat ataatgtaga gtattaaata
    16741 aaaataactt ttaattattg tatacaggag gagttgttgt ggataacata gaattgttaa
    16801 ggttggcaga agatgccaga caacattctt atgctcctta ctcaagcttt agagtaggtg
    16861 cagctctttt aactaagagt ggtaaagttt atactggatg taatatagaa tgtgcatcat
    16921 taggtggaac aaattgtgca gaaagaacag ctatttttaa agcgatatca gaaggtgata
    16981 aggatatcta taaaatagcc atagcaagtg ataactcaga aaataatgag caaacatatc
    17041 catgtggaat atgcaggcag gtaattatag agtttggttc tgatataaaa ataataacag
    17101 gatatactaa aggagaggtg tttgaacatg atataaaaga tttgttgcca aactatttct
    17161 ctggaaatga tttaaaataa attaaaaatt ataaataaat ttaagtaatt aaaacaagaa
    17221 gataaaaaag ttaggagaca gttaatgttc aaatcaggtt ttgtcagtat agtgggtaga
    17281 ccaaatgtag gaaaatctac tttaatgaat aatgtagttg gagaaaaaat agctataatg
    17341 agtgataaac ctcaaactac aagaaataca atacaagcag tatatacaga tgaagagatg
    17401 cagatagtgt ttttagatac accgggtatt cacaaaccaa aaaataaatt aggtgaattt
    17461 atggtaaagg cggcaacaga agcctttaaa aatgtagatt taatattatt tgtagttgat
    17521 gattctaaaa aaataggtcc tggagataga aagataatag aggatttaag aagtgttaaa
    17581 acaccaataa ttttagttgt aaacaaaata gaccaactag accaaaaaga tgagctattt
    17641 gatataatca aaatgtatga tagagaaggt atttttaaag aaatagtacc aatatcagca
    17701 ttaaaaggaa agaatacgga taccctaata aaagttatac aaaattattt ggaagaagga
    17761 ccaaagtact ttccagatta tatgattaca gaccagccag aaagagtatt aatagcagaa
    17821 cttataaggg aaaaagttct tcactatctt aatgatgaaa tacctcatgg tgtagctgtt
    17881 gaaattgaaa aaatgaaagc gagaaatgat aaggaaatag tagatgtatc tgctgtaata
    17941 tattgtgaaa gagattcaca taaggggata ataataggaa aaaatggtag gaaactaaaa
    18001 ggaataggta aatcagcaag acaagatata gaattacttt taggttcaca aataaatctt
    18061 caattatggg ttaaagtcaa agaaaattgg agaaatttac aaaattatat aaataatttt
    18121 ggatataatg ataaataaag gtgggttata tggataggaa actggatgct tcccaagatt
    18181 tattatatga agtaaaatca ttaattgata ataataaagt attggagtta agagaattaa
    18241 tagaggaata tcatataatt gatatattcg atataatgga aaatttagag gaagacatga
    18301 aaattcaatt atttgaagta cttcctttag atatggcatc ttctatatta gaagaaggta
    18361 gtgtagagtt ttttattagt attttatcta aactggatgt agaacactcg aaaaatatac
    18421 ttgaacttat gtctcttgga gatatggcag acaagttgag tgaacttgaa gaagaacaaa
    18481 gagaacacat aataaatctt ttgaatcaag agaatgccga ttatgtaaaa gagttactct
    18541 tctatgatga agattctgct ggtggaacaa tgaccactgg atatatttct ataaataaag
    18601 atatgacagc tcttgaagct atagaccata tgagggaaga agctgaagaa gctgaaacta
    18661 tttattatat atatgtggtt gatgatgaag aaaaactagt tggagtatta tcattaagag
    18721 aattaatagt agctagagat gcaaatatag tagaagattt gatgagtgaa aatataattt
    18781 ctgtttatgt agatgaagat agagaagaag cagtaagatt ggtatctaaa tacaatttaa
    18841 tagcaattcc agtagtagat agacaagaaa aattaaaagg gataataaca gtagatgata
    18901 taattgatgt aatggaagaa gaagcaactg aagatatgta taaatttgca ggaagttcag
    18961 aacatgaaag agaagttgct gaaaaagaaa accctacatt gaaagagcaa ataatatcag
    19021 cacttagagg aagattacca tggttaatta ttaccttagt tggagggttg ttggcttcgt
    19081 taatactttc taacttagac tatataatga accctgtata tgctccatta gtatttttca
    19141 tacctgtagt aattggcatg ggtggaaata ttggaactca atcatcatct gttacagtaa
    19201 taacattatc taataaggat ttaaatttta gcaatgtggt tagagaaggt attgtaggaa
    19261 ttattacagg tcttttgtgt agtataataa caggtattgt aatttacttt gttatgagaa
    19321 agttagacat tgtattaatt gtttcaatat cattatttat aaatatggtt ttgggtgcaa
    19381 caataggtgc gtttatgcct gttttattaa aaaaaatgga tgcagaccca tctacagttt
    19441 catcaccaat tatatctaca gctcttgata taactggcat agcagtttac tttataataa
    19501 caacagcatt attgtcaaaa attgtttaat aactttatgt atgatttatc ctcttcttac
    19561 taaaaatata agtataaatg taagaagggg gttttttaaa aatgaacaat atcagttggg
    19621 aagtttttaa aaaaacaggt agtatagatg catatttata tttctgtgat tgcaaaaaca
    19681 tacatgaaga aattgaagaa gtaagggaga aaaaagaaga cgatgataat cctgaacacc
    19741 caggggatag tacttaaggc tataaggtat aaggaaagtg acataatact tacactattt
    19801 acaagaaagc ttggaaaagt atctgcaata gcaaaaggag ctaaaaaaaa taagagctct
    19861 ttgctttcat catcacagtt attttcttat agcaatttta ccttaaaaaa acaaggtaat
    19921 atgtacaagg ttactcaaag tgaaataata aaaagttttt ataatatatc ctatgatata
    19981 gaagcatttt cttatgctac atatattaca aaattagttg aaaattctat acttgaaaat
    20041 cagactaata ataggctatt tatattactt gcacaaactc tgtatttgta cacacaggat
    20101 aatacagata ataggtttat aacagcagca ttcgagttaa aatttttaga ctatatagga
    20161 tttaaaccta tagtaaataa atgcattaat tgtgaatgta aaattttaca aaattctgtg
    20221 tttaatatct atgaaggtgg tatactatgt agtaagtgta gtaaactttt tgataataat
    20281 ataaaattag acttaacaac tgtaagtctt atggaatatg tattaagaaa tgatatttta
    20341 acatgtagta aagctaaagt atcaaaatat ataacacatg agcttgagaa tattctagac
    20401 aagtatttga aagtttatgt tgataatatt aattttaaat cattacacgt attgcaaagt
    20461 gtaaaaaata ataagggagt ggataatgat gagtagtaat ataactattg aaatggttga
    20521 ttcagtcttt gaaagagttc caggagcaac ttatgcagaa gcaaaagagg cattagtaat
    20581 gtgcaatgga gatgtaattg aggcagtaat atatttagaa aataaaaaaa atacttgtaa
    20641 ttgtaaagca aaatctgcta aagaaactat ggaagaagta tttggtaaag atggagaaaa
    20701 tataaagttc caattaaaag agcttcttag aaaaagtagt gtagtgagaa taatagttga
    20761 aaaagatgga aaaactatga tgaacattcc tttaacagtt ggagttgttg gattagcttt
    20821 tgtaccatta gctactttag ttggattatc agctgctgtt ataagtaaat atcgtataaa
    20881 agttcaaaat gaagaagatg gagaaattgt agacttaggt gagttgaatg aagaaaaact
    20941 tcatgttttg aagaatatga taactaatgc agcaaaagat gtaaaggatg tagtagttga
    21001 caaaaaagaa gatgataagg atgtaactgc tgaccttatg aaagaagaat ctgaaaagaa
    21061 taaagaagac gaagacgaaa ataaatagat ataaagtgac ttttaaaaaa tgaagagaag
    21121 ataattataa gaattatcgc taacattgac aaattttata gaatttgata ttctaaaagt
    21181 tgtagaagaa ctattaaaat agcctttcgt gtagacgaga ggctttattg ttataggagg
    21241 gatttttatg aattttcaag agatgatact ggcgttgcaa aaatactggt caaagcaagg
    21301 ttgtatcatg atgcaaccct atgatattga aaaaggtgca ggaactatga atcctaatac
    21361 ttttttaaga tcattaggac ctgaaccttg gcaagtatgc tatgtagagc cttcaagaag
    21421 accagcagat ggaagatatg gtgaaaaccc aaacagacta tatcaacatc atcagttcca
    21481 agttatacta aaaccttctc cagataatat acaagagttg tatttggaga gcttaaaaga
    21541 aattgggatt gacccaagtg agcatgatat aagatttgtt gaagataact gggaagctgc
    21601 tacagttggt gcctggggac ttggatggga agtttggcta gatggtatgg aaataactca
    21661 atttacgtat tttcaacaag taggtaatat tgaatgtgag cttgagacag gagaaattac
    21721 atatggatta gaaagattag ctatgtacat tcaagaagtt gatagtgttt atgacttaaa
    21781 atggaatgat aaaataactt atggtgaagt atttaataaa gctgagtatg aaaactcaat
    21841 gtatgcattt gaattatgtg atgcagatat gttgtttaat ctgtttgata tatatgaaaa
    21901 agaagcatta agacttatgg aaaatggact tgtaatacct tcatatgact atgtgttaaa
    21961 atgctctcat gcatttaata cattggatgc aagaggtgca ataggagtta gtcaaagagc
    22021 ttcatttata ggtagagtta gaaatatggc aaagactgtt gcagaaactt ttgtaaaaca
    22081 aagagaagaa atgggattcc ctcttttaaa ggacggtgac aaataatgaa aaattatctt
    22141 ttatttgaaa tcggtgttga agaaatgcca tcaagatttg taggcagcac actagaacaa
    22201 ttaaaaaaca atcttagcaa attatttgat gaaaatagaa tagcttttga taaaataaat
    22261 gcgtatggga caccaagaag acttacttta gtagtcgaag gattgagtga tagacaaagt
    22321 gatttagaag aggaaataaa gggtcctgct aagaaaattg ctgttgatgc agaaaataat
    22381 ttaacaaaac cagcattagg atttataaaa agcaaaggat taagtgaatc tgatatatac
    22441 tttaaacaag ttggaaaaga tgaatatatc tttgctacta taaagcaaga tggaaaagaa
    22501 acttcagaag tactaaaaga tatattacca gatactataa aatctgtagt attcccaaaa
    22561 gctatgcgtt ggggaggaaa aaatcttaag tttgcaagac ctataagatg gatagttaca
    22621 ttgttaaatg ataaagtatt agagtttgat ttagaaggaa tagtatcttc taatgtaaca
    22681 aaaggtcata gatttctagg tgaaagtaca atagaagttg attctataga agactatttt
    22741 gaaaaattag aaaaaaatta tatagtttta gaccaaaata agagaaaaga aatgataaga
    22801 aaacaatgta tagaagtggc aaattcttta ggtggagaag ttgaatttga tgaagattta
    22861 cttgaagagg taacttactt agttgaatat ccaactgctt tttatggaga atttgatgta
    22921 gattatataa aactaccaaa agaagttgtt ataactccta tgaaacaaca tcaaagatat
    22981 tttccagtac ttaaagaagg aaaactgtta cctaacttta tagctgttag aaatggtgat
    23041 tcttatagaa tagacaacgt taaagctgga aacgaaaaag ttttagaagc aagacttgca
    23101 gatgctttat ttttctatag agaagatact aagagaaact tagaaagcta tatagaaaaa
    23161 ttaaaaactg ttgtattcca agtaaagcta ggaactattt atgacaaaac tttaagactt
    23221 gaaaaattaa gtgctgatat acttgacagt ttaggtttag tagatgaaaa ggcagatact
    23281 ataagagctg ctaaattatc taaggcagat ttagttactg gaatggtagg agaatttgat
    23341 gaattacaag gttttatggg aaaagaatac gcaaaagttg gaggagagaa cgatgttgta
    23401 agtgaagcta tttttgagca ttatcttcca agatttgcag gagatatact tcctaagact
    23461 aacgcagggg ttgccttatc aatagctgat aagttggatt caatagtagg tttctttgct
    23521 ataggaatac aaccaacagg ttctcaagac ccatatgctt taagaagaca agctttaggt
    23581 atacttagta tacttatgga cagaaaagtt gctgtggata taaaagtact tattgaacat
    23641 gctttagata attattctga attagatttt aatagagaag aagttgttga acaaataatg
    23701 aatttcttca atgagagagt aaaaaatctg tttaaagatt taggaataag atatgatgtt
    23761 gtcgatgctg ttttaagttc tgatataaat gatgtttctg acatgcatat gagagcttta
    23821 gagcttaata attggctaga aaaagatgaa ttagtggaga tgcttactgc atttaacaga
    23881 gtttctactt tagctcaaaa agcagaaagc gataaaattg atgaatcttt attaaaagaa
    23941 gatgctgaaa ttaagcttta caatgaattt aaacaagtaa aggtaagagt taagcaatta
    24001 ttaaaagata agaaatatgg tgttgcttta gatgaatttg catctttaag accagtagtt
    24061 gataatttat ttgatacagt tatggtaatg gataatgatg aagctataaa gaacaataga
    24121 cttgctttac ttaaacaagt atacgatact atgctagaaa tttgtgattt atctaaaatt
    24181 gtttataaat aaaattcaag ctatttataa ataatgaggc tatctcaaaa tagagataag
    24241 cctctttttt atttttgaaa tgataaaaat atatttaatt gcatacaaaa aagagtacct
    24301 catgaactga aaagttaatt ttgaggcact gctacttttt ataagtaaat gtaataaatc
    24361 atattttata cttatcagtt attttgttta agatgtcatc atttgaattt acccattcat
    24421 aaaaatttcc tgttgaagcg tcagatattt tataaagcat ttcaggaata tcgctagctt
    24481 taaaatctcc aagtgatttt cctaatctag tcttgcctat ttcaaagcta cctccaacaa
    24541 ataattcaaa tgcatctgta gatacaccat caacattctt ctttttacca cataaaccta
    24601 tactaccaac ttggtgaaca ccacatgaat ttaaacatcc tgatatatat aacttaggtg
    24661 ctttatttaa aatttcttca ttattctgta atctaaaata atctatgatt tcatgtaaca
    24721 ttttctgact attttgtata cccatttggc aaattggaac acctatgcaa gatatgcttt
    24781 gttctaattg agaattacaa ctaatagttt tacttatttc taatattctt tttgcttcat
    24841 ttccatttaa atttaaaata tataatccct ctgtcattcc tagtctaatc atagggtttt
    24901 caatattatc taattcttta agtaaagtat ttaaatcctt tgttaacaaa atacctccaa
    24961 ctggatgtat atacacagtg tataaaccat cttgtttttg aggtattaat ctggaatctt
    25021 gtatacatat tttaactcca gatttagagt agtctatttc ttcaatattg agttcaagac
    25081 caccattttc tttttctaat ttatagtatt ctttaaattt ttttaagaat agctcttcac
    25141 caagtgattc gaccatatat ctaactctag ctctgttttt attttcataa ttgccataat
    25201 ccataaagaa tttaatcata ccttcaacac aatataatgc atctttcggc tttataagtt
    25261 cgtcaagctc taaagcaact ttgggttgtt tacctagacc tccacctaag aaaactctga
    25321 aatattgttc atcatttttc aaagtagcca caaatcctaa gtcttgtata ctacaatgag
    25381 cagtatctgt gtaacaatta gaataagaaa cttttaattt tctaggtaga tggtatgtag
    25441 tagcattttt gataaaatat ttatcagtag caactgcata tggagtaaca tcaaaggctt
    25501 cactagggtc aactccagac aatggagata atccaacatt tctaggataa ttaccaccac
    25561 cacctctagt gaatatatcg ttttttattc cttctttcat gatatcgaca atcgaatcta
    25621 tagataaatc gtgtaattgt atagcttgtc tagttgttaa gtgtatctta tctaagttgt
    25681 gtttaatagc catttcatag attttattga gttgatgatg agataaaact ccagcagata
    25741 ctctaagtct tatcataaaa gattttttgt ctctttgagc ataaacaccg tatccaccag
    25801 aaaatccctt atattgcatt ttatttattt ctccatttaa aaattttgaa gaatattctt
    25861 taaaattagg tatttcattt aacaatattt ttttaaaatt gtccattatt tcacctcatt
    25921 attagtcttg tataaaaata tatctattgt ataatagata tatatattat tatctagtca
    25981 ggataaaaat ataacagaat aattctatta aaagtaagaa taataaggac atagtgagta
    26041 ttttgttttt attttacaaa ttaagtataa tatttttgtt ttataaaata taattaagat
    26101 aaatagataa ttttgcaatt ttaatatata atgtttagta gagtgtggga gtagcactta
    26161 atatttgcag aaggagtttt tgtatatggg agaaataata aatgcattag atagagccct
    26221 agacataatt ttgctgttat atcatgaaaa gagagaaatg ggaatcactg aaataagcaa
    26281 ggctatggga gtatataaaa gtactgtaca tagaactttg gtcactcttg aaaataaagg
    26341 ttttgttata caaaatgcag aaaatggtaa gtattggcta ggaataaatt tatatgcaat
    26401 aggtatggtt gtgggagaaa aaatgtcatt gactgaaatc gtaaaaccat atacgaaaaa
    26461 attaaatcaa gaatttaacg aagtagttaa tgtatctata ttagaagaaa gagctcaaga
    26521 ttctccaaga agtattataa tacataaaga gtatggttca aatcaattac tttcagtaaa
    26581 tccatccgtt ggctcaagta gtgaatgtta ttgttcggca gtgggtaaat gtcttatggc
    26641 atttaatgat tctatagatt ttgaaaaata tagaaaaaca ccaatacaca agtatacaga
    26701 acatacaatt gataattggg atgatatgat gcttttttta gcaaaaataa aagaacaagg
    26761 atatgctata gatgatgaag aactagaaca tggactaact tgtattggag cacctatctt
    26821 agataaaaac aataaagcta ttgcagcaat aagtttgtca ggtccaacta taagaatgag
    26881 agaaggggac tttgagtata agattaaaag agttatagaa actgcaaaaa gtatatctga
    26941 actttttaga tgatttttta atctagataa gaaaaagagg ttatatttaa attgttattt
    27001 gtaaatatca gtttaaatat agcctctttt tctttaatct aaaattaaat attcttaaaa
    27061 tttaaaatgt tattgaaata tataagcgaa tgtgttatct tttaattaag caataaaaat
    27121 agaacaaggt tctatactat agaacaaaaa ttaatgcata tatagtattt atataaaata
    27181 taaatattat atatttaaaa ttaagtaaaa atattacata aaggggagat ttgagaaatg
    27241 gaaaaagtca agttgagtgt tgaaagatgt aagggatgct atctttgtat tgaagcttgt
    27301 aaaaaagatg caataagtct ttctgactac acaaataaaa aaggctttgt aactattaaa
    27361 gtcgatgaag aaaaatgtat tcaatgtggc gcttgttata caatgtgccc tgacttagta
    27421 tttgagatat tataaattat ttttattaga ggggagaaaa ggtatgcaaa gtttattaat
    27481 taaaggaaat gaagctttag cagaaggtgc tatacgtggt ggctgtagat tttatgcagg
    27541 ttatccaata acaccacaat cagaaatatt agaaatgatg gcatggagac aaaaagaagt
    27601 tggaggagta tttatccaag gtgaatcaga aatagccagt gtaaatatgg ttatgggagc
    27661 aagtgcactt ggagcaagag ctatgacttc ttcatctggt ccagggtttt cattaaaaca
    27721 agaaggtatt gcatattggg tatcagctgg tttgccagca gttgtagcat gtgtacaaag
    27781 atatggtata ggtgatggat ttataggagc tggtcaagat aattattggc aagcagtaaa
    27841 aggtggcgga aatggagatt atcatttaat cgtgttggca cctaatagtg ttcaagaaaa
    27901 tatggatatg gcatatgaat cttttgaact agctgaaaaa tatatgcatc cagtacttat
    27961 actttctgat gctacaatag gtcaaatggt agaaccttgt atagcaccac caatgaaaga
    28021 atacgatatg gataaagctc catgggctgc aaaaggaaca cctgtaggag aaaaaaataa
    28081 agtaataact gatataacat actttgaaga ccaagataca tggcaaaaag ggtatataga
    28141 aaaaatgaga aaagttcaag aagatgaaca aagatgggaa gaaatagaaa tagaagatgc
    28201 tgaagtggtg tttgtggcat atggtatttc atcaagaata gcagaaggtg cagttagaga
    28261 tgctagagca aaaggaatga aattaggact aataagacca aaaacattgt ggccttaccc
    28321 aagaaaggca tttaagaaat taagtgattc attgaaggga cttgtaacag ttgaaatgaa
    28381 catgatggca cagatgaagg aagatgtaat tatagcatgt gataatagat ttccagtata
    28441 ttcagtagca acagctcaat atatggcaga agtaaatcaa ttaatagatt ttgctcaaca
    28501 gattatagat ggaaaagcta agcaagagga ggtattttaa ttatggctaa aaaagcacca
    28561 gcaataatga tgtgtgaaaa tatgttctgt ggaggctgtg gacactctat agtcgataga
    28621 attattggag aagtattaac agaaatggat atatgtcaag atacaattat atgttcagat
    28681 ataggttgca accatatgtt ccaatttagt atgaatgtag atgttgtcgt tccacctcat
    28741 ggtaaaatgg gagcagcaat ggcagctatg aaaagagtca gaccagataa atatatattt
    28801 acaatagctg gagatggtgg agcttatgca atagggcttg gagaaactat gtcagctgct
    28861 actagaaatg ataatgtaat atttcttgtt atgaacaata acatgtttgc aatgacaggg
    28921 ggacaaatgg ctccaactac tgtagaaggt caatatacag cttctactcc agcgggtaga
    28981 aattacgcag atcatggttc aacatttgat gttgtaaaag ttatgggaaa tttagatata
    29041 gcatatctag cgagaggaac aataactaac cctaaagaaa taaatacaac aaaaagatac
    29101 atcaaaaaag ctctagaaaa acaaaaagaa aagaaaggtt tttgcttaat agaagtgctt
    29161 gtaccatgtc caacaaactg gggattaaag ccagtagatg ctatgaagag aattgaaaat
    29221 gaacttgttc caaagtatcc acttggagaa tttattgatt agggaggaaa taatatgttg
    29281 aatgaaatta tatgtgctgg ttttggagga cagggagttc ttacagcagg aaaaattata
    29341 atgtatgttg catataaggc agaaaagaaa attacatggt tcccatctta tggaaatgag
    29401 atgaggggag gaagtgcaaa ttgtaatgtt gttgtaagtg atgaaaaaat tgcaaatcct
    29461 tgtgtaaaaa atccagatat attattggga atgaacagcg tatcagtaga taagtatgaa
    29521 agtttaatga aaaaaaatgg ttatatgttt gtcaatgaat cactagtaag taaggataga
    29581 aaatacagag aggatattaa tgttataaga gtacctgtta ctgagatagc acaaaattta
    29641 ggtaatgaaa aagctgctaa tatttgtatg ttaggtgcta tggtaaagaa tttggattta
    29701 tttacaaaag aagaatttga aaatggtatg tgtgaatact ttgaaaatca aggcaaaggt
    29761 aaatttaatt ctaaaaatgt agaagcattt gaagctggat acgactacga gtgaatattt
    29821 taaatattta aaataattat tgaaatactt atttttatat gttagtattt aattaataaa
    29881 atatagaacg tcgttctgga caataggaca aaatctttgt gcttgaatat aagaacgacg
    29941 ttctatgtaa tagaattagg aggtgttaaa ttgaaaattt tagtaataaa tcctggcggt
    30001 acatccacaa agatttctgt tttcaaagat gaaaatgaaa ttttgaaaaa aaatataacc
    30061 catacaagag aagatttaaa aaatttttct aaagtatttg atgaatatga ttatagaaaa
    30121 caactgatag tagatatttt aagtagtgag aatcatagta taaattcttt tgatgcagta
    30181 gttggtcgtg gtggacttat gaaggctata aaaggtggta cttatactgt gagtgaagaa
    30241 atgattgagg atatgagaaa tgaaataaat ggggaacatg catcaaattt aggtgcatta
    30301 ttagctaaaa ctatagctga tgaaataggg gtacaatcat ttgtagtaga cccagtctct
    30361 gtagatgaat ttgaagatgt atcaaggatt acaggaatat cagatataga aaaagcaagc
    30421 tggctacatg cattaaatca caaagctgtt tcaagaaaaa ttgcacaaaa actaggtgga
    30481 aaatacgaag attataactt cattgttgct catttaggtt caggaatatc gattgtagca
    30541 cataaacaag gaaaaatgat tgatggaagt ggtggaaggt ctgatggtcc attttctcca
    30601 gaaagaagtg gtggattgct tgcatatcct cttatagaat tgtgttactc cggtaaatat
    30661 actcatgatg aaatggtagc aaaagtaagt tcgattggag gcatgtacga ctatttgggc
    30721 acaaaggaca tgatagaaat tgaaaacaga atcaagcaag gtgatgaaaa ggcaaaactt
    30781 ataatggatg cattcattta tcaagtagca aaagaaattt ctatgtatgg agcttcactt
    30841 tcaggcaatg tagatagagt tattttaaca ggaggaatat ctcattcaga aatggtagtt
    30901 aaaggagtca tagaaaaagt ttcatatata gctccagtag aggtagtacc aggggagatg
    30961 gaaatggaag cacttgctct tggagctctt agagtactta atggagaaga aatagcaaaa
    31021 acttattaat atttaggaga aggagtatgt taaaagattt taataattta ttggaagaag
    31081 ctaaaaagct tgaacctgtt atagtaagtg ttgctgttgc agaagacaaa gaagtattaa
    31141 aggctgtgaa atcagcaaca gatttaaata ttataaagcc aattctcgta ggagataaag
    31201 ataaaataga aaatatatca aaagaaattg gattaaaagg tcatgaaata ataggatgtc
    31261 aaaatcctga tgaatcagtc aaaaaagctg tagagatagt aaaaaataag gaagctaaag
    31321 ttcttatgaa gggattagta aatacgagtg tgtatatgag agctatatta aataaagaaa
    31381 ctggtctcag aacaggaaga ttattgagtt tacttgcagt atatgaatta cctcaatacc
    31441 acaaacttct ttattgtact gatagtggaa taaatgtatc accaaatcta aatcaaaaaa
    31501 aagatataat ggtaaatgca cttcttacta tgaaaagtat aggcatggaa aatcctaaaa
    31561 ctgcgatact tactgcaaat gaaatggtag accctaagat acaatctaca gtagatgcaa
    31621 acctacttgt tgagatggaa aaatcgggag aaataccaaa ttgtatagtg gaaggtccaa
    31681 tagcatttga tgtagcattt gatgcacatg cagcagaaca taaaggtatc aaaagtaata
    31741 ttgctggtga tgttgattta ctagtatttc caaatataga agcagggaat atattaggaa
    31801 agtcatggct tagatttaat aaagctaagt gggcaggtat agttttagga gcaacaaatc
    31861 caataatttt aggttcacgt tctgatacag ctgaaattaa aataaattca atagcattag
    31921 cgtgcttagc atcacaataa gaaatcatta ttaaggagga taaattatta tgaaatatgc
    31981 aaaaagaatg gatatggtaa aagcttcagc tataagagat tctcaaaaga aaatagctca
    32041 gaaggtagca agtggtggaa cggttatatc ttttgctgca ggacttcctg acccaaattt
    32101 atttccaata gaagaaataa gtgaagtaac tcaaaaagta cttaaggaaa gaggtaagtt
    32161 tgccctagca tatggaccta ctaaaggtga agatgagttg ttagagctat tagtaaaaag
    32221 aatgaaagaa gaggaaaata ttgatacaaa agcagaaaat attataataa ctactgggtc
    32281 acaacaaggg atagcattaa gtgctatgat tttattagaa aagggagatg tagtagttac
    32341 agaaaatcca agttacttag gtgcaataaa tgcatttaga ccttatgaat gtgatttttt
    32401 aggagtagat acagacgaag aaggaatggt aacagatgat ttagataaat tattatctga
    32461 aaattctaac ataaaggtga tatatgtaat accaacattt caaaatccta caggaaaaac
    32521 atggtcttta caaagaagaa aagattttat ggaagttgta agtaaatatg atgtcatagt
    32581 aatagaggat aatccttatg gagaaataag atttacacca gaacctctac ctacacttaa
    32641 atctcttgat actaaagata aagtattgta ccttgggtca ttttcaaagg tattatgtcc
    32701 aggatttaga gttgcttgga tgtgtggtaa tgtagacatc atagataagg cagaactttt
    32761 aaaacaaggt atagatttac agtcaaatca attttctcaa gtacaagtaa tagaattttt
    32821 aaagaaatac aacttaaatg aacatataga aaaaataaga gaagagtata aaaaaagatg
    32881 cttactaatg ttggatgcta tgaagaaaca cttcccagag gaagctaaat atacagagcc
    32941 agatggagga atgtttatat gggttgaact tcctgaaagc ataaatgtag atgatttatt
    33001 agataaagcc attgatgctg gagttgctta tgtttctgga gagtctttct ttgcaaataa
    33061 tggtccaaaa aatactatga gacttaacta tacaacaatg tcagaagaac aaattgttaa
    33121 aggtgttgag atacttgcag aagttataaa aaaagaactc aattatgctt gtgaataaat
    33181 ataaaaagtg agggtttaaa tatgggagaa aaagaaaacg gtataaagtt tggaaagtta
    33241 atgtttcaac tcgttattat agcaatatct tgggaattag tttatattat accatttatc
    33301 caatatacat tatatgatcc aatcctaaaa gcattagaat gcacaaatac tcaactaggt
    33361 tttttactta caatttatgg gttaggtaat atatttggag ctcctatagg aggctggtta
    33421 gcagatagat ttgattatag aaagataatt ttaggttctg tatttcttaa tggagtagta
    33481 tcatttttgt ttgcatttaa tatgaactat ccatttgcag ttgctacttg ggtgggttgt
    33541 gcaatcacat cattagttat gaactatcct tctatggtaa agatacttag agttatagga
    33601 aaagataatc aaggtaaggt ttatggattt aatgaagcta tggttggagt ttctggcgta
    33661 gtaatgggag caatattctt atatatttat acatgctttg caactcctac attgggtatg
    33721 agatgggtaa tgatatcttt aggaatatta tcaatagcaa tgtgtccagt attatggttt
    33781 gtaattaaag atgtagatat taaagaagaa aaagaagaag caggtgaaaa aatgtctgct
    33841 ggtgatttta taacagtact aaaatcacca aatacttggc tagtaggaat aagtatattc
    33901 tgtgtatatt cattcacagt tacaatgtct tattttacac catatataac atctgtactt
    33961 ggaggttctg tagcactatc tggtgcatta gcaataataa gacaacatgg tctaaaattg
    34021 tttggagcac catttggagg atattgtgca gacaaattaa aatctcctac aaaagtactt
    34081 ttaccaatat atgtatttgg tatagcagta atagttttat tcctagtatt acctgcatca
    34141 actccaatga ctatattcat agctctaaca tttgtagtag gtatattagg ttacatggga
    34201 aaaggtatat attatgcagt acaagatgaa gtaaaagttc cagttaaata ttcagcaact
    34261 actataggta tagctgcagc tttaggattt tcaccagatg tattccaatt tgcgttaata
    34321 ggacactgga tagatactta tggcaataaa ggttacacat atacatttat attccagata
    34381 gcaattttag ttattggaat actatcttgt ttatatatat taaaggttaa aaagagaaga
    34441 gcaagtaaat tagaagctta aatattcaat ttggtgatta agtacatgta aatacaatac
    34501 ttattttgat gaatgtgatt gtataaataa cataaaaaat atcaatagat taagttttat
    34561 aataatgttt agagaataaa atataaaaat caaaatgtta tcaagttttc taagttttag
    34621 cataaagtta cccctaaagg tataaaattg agtagtaatt attatctatt ttcttactta
    34681 cataaaccta tttaatttaa attctaagag aatttcaaaa tagatggcag ttgtactcaa
    34741 ttttatacct cataataaat acgaaaattt aaaatctaac tagatactat ttaattatat
    34801 attaa
//
LOCUS       DAMJVM010000034        34559 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.034, whole genome shotgun sequence.
ACCESSION   DAMJVM010000034 DAMJVM010000000
VERSION     DAMJVM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34559
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.034"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            139..696
                     /locus_tag="PC523_001725"
     CDS             139..696
                     /locus_tag="PC523_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2475253.1"
                     /translation="MRTLKYAILGLLNRNPMTGYDIVKEFNFQLAEFWNAKHSQIYPE
                     LKKLVNEKLIVYDIKISGDVLEKKLYTITEKGKDEFLKWLEKDEPMERTPKDIFRLRM
                     YFSNNLDIDTRLILLEHQLLQHQERIAFLYSQMERYSEVPPLNSNDFGDYIVLDGAII
                     REQGAIKWLTNCINHHKNLDNTTLK"
     gene            1120..1869
                     /locus_tag="PC523_001726"
     CDS             1120..1869
                     /locus_tag="PC523_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2475254.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     DKPFIFVCPTYAWRLPIVVTDFIKKTKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGAIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            complement(2029..2394)
                     /locus_tag="PC523_001727"
     CDS             complement(2029..2394)
                     /locus_tag="PC523_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2475255.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILITLLESDFN
                     GIRVGEITEKTHLSRPAVSHHLKILKEAEIVNVRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(2410..3186)
                     /locus_tag="PC523_001728"
     CDS             complement(2410..3186)
                     /locus_tag="PC523_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2475256.1"
                     /translation="MKYQHIIKVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPQKAITLTGFDEPPIELTNKPNLDSDELLMAIKSRRSIRKFKDKEVSLE
                     IVKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDKFFFKNAPIAIMIITKDKISGSLAASNMSLMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEAANVRYL"
     gene            complement(3605..5032)
                     /gene="ascB"
                     /locus_tag="PC523_001729"
     CDS             complement(3605..5032)
                     /gene="ascB"
                     /locus_tag="PC523_001729"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF2475257.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDKHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPRGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEIDSNNLVGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGIAPEMESGDIELLNKYTVDFISFSYYNSRCVRTDENAGDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     IDYLREHIKAMHDAVYIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRTKKKSFEWYKKVIASNGEDLY"
     gene            complement(5093..7021)
                     /locus_tag="PC523_001730"
     CDS             complement(5093..7021)
                     /locus_tag="PC523_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2475258.1"
                     /translation="MGKYKQLAQEIVKNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDNSSANSETKKSGSILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKFIPDLVKFFFVPMLTLLITVPVALIV
                     LGPIATFGSTVISQVVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVASPIKGEVLKLS
                     EIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDAGVEVLIHIG
                     LNTIQLEGRCFEACIKQGDRITKGQTILNFDIDAIKELGYSVVTPIVITNSSQFLDVV
                     ETGRKNIELEDNLITVLF"
     gene            complement(7166..8008)
                     /locus_tag="PC523_001731"
     CDS             complement(7166..8008)
                     /locus_tag="PC523_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2475259.1"
                     /translation="MIINKILNNNVVITLDDNDEEIIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEVSNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGVKLPEDEAGFIAFHI
                     VNAQLSEGHTFASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLN
                     NAHEGGTDKELLSIIKSKYNKEFECVVKIKDFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNI"
     gene            complement(8180..8326)
                     /locus_tag="PC523_001732"
                     /pseudo
     CDS             complement(8180..8326)
                     /locus_tag="PC523_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893832.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(8426..8875)
                     /locus_tag="PC523_001733"
     CDS             complement(8426..8875)
                     /locus_tag="PC523_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2475260.1"
                     /translation="MEIREILSEESIILDLKSTTKEGIIQELVDKLYDTGSIDLKSKF
                     IKALKRREKIGSTSIGYQMAMPHGKTKYVKKASVVIGKSKKGIDWPSIDGLPVKVIIM
                     FAMPQSGDNEDMTLISKIAYILGGKDISERLLNANTKKEILDIFYEV"
     gene            complement(8865..10772)
                     /locus_tag="PC523_001734"
     CDS             complement(8865..10772)
                     /locus_tag="PC523_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2475261.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKIIE
                     KYLETFNIKIDIKRGYGILIESLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISKLTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTISQPYYINLITHILICLKRIEEGNQIESQEEREVSVDNLDELVYQNVTALIGKIEN
                     RYGVKITKDEIMYIYIFLVSSGFSSESNNSYNDESYRLSESEKFSSIMIENMSQFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSNRNLDDVDFVISTVNLDLKDKLHIVVSVLLMESDISNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVMQMESLIGVDLGSKYKSISLGKDLNIYIHNQSKLN
                     KGIMNVNNINRTIDIYLETSNQSFLKNILMEISNLYRQKSYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            complement(11039..12934)
                     /locus_tag="PC523_001735"
     CDS             complement(11039..12934)
                     /locus_tag="PC523_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2475262.1"
                     /translation="MIGQILRSDCIVLDKQLQTKKDVIDYMANHLYHGNYINDKEKFI
                     VDINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDKEITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMDENLLKHLLSAETPYEILSIFNNKAVESGDSSDNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQMSRFIGKKVLQKKVADAKNVSLAKNLIINAENGMAKEIKPGGRTLEDSK
                     EILQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFSTG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSSPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVEVLGA"
     gene            complement(12970..13848)
                     /locus_tag="PC523_001736"
     CDS             complement(12970..13848)
                     /locus_tag="PC523_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDF2475263.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREVVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FIEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCREVLEKNSSLYEPDTIYPPCIDAAREVIEFKMN
                     LFNTIDKLKCFYKNKN"
     gene            complement(14400..16094)
                     /locus_tag="PC523_001737"
     CDS             complement(14400..16094)
                     /locus_tag="PC523_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2475264.1"
                     /translation="MIKKIKRSKFTPKNVISIILYSILFWILIGYIIIPIGNTFTQAF
                     QGKGGYSFEVFKEYFSNTNNLKVVSNTIILGFGSVLVCGIMGISLALYMTFVCDKHKK
                     IIHILLLSPMMIPGVIMVIACIQLYGESGIVTKSIEILLSFIKKPYDFSGLKGVLFVV
                     AYTQYVYFYLNVYVALKYVDYSTVEAAKGMGASKIRVFFDVIWPVITPAIITSTIITF
                     ASGISSFSAPNLIGGGFKVLSTQIVRSKANNHMDIASVQVIILLLMGISFMILLQYYG
                     KKYGVVASLKSQGYKVHNRKKDLFTFVCKVIIEIQIVMIMLPIVTIVYLSFVSTHSIM
                     TTIFPRDFTLENYQMIFQKSRVLQPILNSLKMSLMAVVIGLIITIPVSYLVIKKDTTY
                     NRLAKFIIMLPWSMPASAIAVNLINTFNQKSIFAFNESLIGGFYIVPIAYTIYALPLL
                     FSSNEVAMKSINLGLEESSRSLGAGRIKTFYNVIVPNMMPGIISGAILVFIRTIGEYT
                     MSALLYGVYNRPISISIVTNMQEFKIGVSMAYGVLVIGICYIALGIIFKLDRKRFT"
     gene            complement(16096..17088)
                     /locus_tag="PC523_001738"
     CDS             complement(16096..17088)
                     /locus_tag="PC523_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2475265.1"
                     /translation="MKNIELKEINHYYGKEKVLYDINISIKEGEFFTLLGPSGCGKTT
                     ILRIIGGFIKPSSGSIYVGDKNITNLEPENRNMGTVFQNYALFPNMTVEENVSYGLKI
                     KKLSKKIIKEKCDNYLELAGMKDFRKKKIDELSGGQQQRVAVARSLATEPTMLLLDEP
                     MSNLDIALRIKMREEIREIQQKIGITTLFITHDQQEALAISDRIAVMDKGKVLQIGVP
                     MEVYKKPLNDFVANFVGTSNCIEKEDYVNFNIEKETKPYIYKRPEEMVLLQNTNQSGF
                     IKVKVESKNFLGAILEYTVSNNGKKYQVTELNRLNNSNKLNVGDMAYLGVLQGN"
     gene            complement(17107..17964)
                     /locus_tag="PC523_001739"
     CDS             complement(17107..17964)
                     /locus_tag="PC523_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475266.1"
                     /translation="MGLKISDYKLLCNKGNGDFNEDIVGLSPYGAWVLDGATGLNNKN
                     LVSTESDAKWYVSWWNSYLHENMEREDSLKVIIKEGIEEITKEYYKLTYDKEVEAIDI
                     PSCSCSILKFYKDKIEYLLLGDCTLLVNHNESVKILKDETLCRLDKKIFDFMQEAKDV
                     TNLTLKERKAEVMPIIIENRLKKNTVGGYWALEFSKEAVDNCISGFIDIEYKMKIMMA
                     SDGFSCGYDRYKIFDKNEMMKIAEIKGIDYIYRKVRELEKDDEMAIEFPRFKIYDDSS
                     CVYLEIYKY"
     gene            complement(18242..19285)
                     /locus_tag="PC523_001740"
     CDS             complement(18242..19285)
                     /locus_tag="PC523_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDF2475267.1"
                     /translation="MKRKIAVVLLTVAMLLSLVACSNDNTSKSDKLEDTLVVYTTHGE
                     DMLGAISEEFTKETGVNVEFINLKGELADRVRSEKDNPQADIMFGGDTSTYMQLKEEG
                     LYEKTKPSWNNELDKSYKDSDGYWYGTIKTPVMIFYNTKLLSADKVPKDWSDLTKEEY
                     KDLIVTRDSLSSSMRSTISNLINYYSNTKSEEEAWKYLEDLNKNIKNYYNSGSVMYSA
                     IGKGEAAISVAVLSDIIENKEKNNMPLEVVDAESGSVVITDCVAALKNAPHPNAAAKF
                     VDFVGSAKVQAMVANDFNRIPTLDSALKDSPKWMSKEYKAMDVDWSVIGKNQSNWIEK
                     WETDIIDNNKTIK"
     gene            complement(20251..21684)
                     /locus_tag="PC523_001741"
     CDS             complement(20251..21684)
                     /locus_tag="PC523_001741"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF2475268.1"
                     /translation="MKSNQEFLWGGAVAAHQVEGGYNKGGKGISIADVMTAGTHTISR
                     KVTDGVIEGLNYPNHEAINFYENYKEDIRLFAEMGYKCFRTSIAWTRIFPNGDESIPN
                     EDGLKFYDDLFDELLKYNIEPVITLSHFEMPYHLVKNYGGWRNRKLIDFFVNFCEVVM
                     SRYKDKVKYWMTFNEINNQSITTNPIYAFTNSGIIYEEQEDKEEVMYQAVHYEFVASA
                     KVVKIGHEINPEFKIGCMVAAIPSYPYSCNPEDMIKFVESNREQLMFTDVHVRGHYPR
                     HTLKLWERKNYNLDITDEDKKILKEGTVDFIGCSYYLTTVVSADKTMKTTGNDSAGKA
                     DVVENPYLKTSDWGWNIDPVGLRFYLNQLYDKYELPIFIVENGFGAEDVLKSDNTVDD
                     GYRIEYLASHIREMKKAIEIDGVDVIGYTVWGCIDPVSFTTGEMKKRYGFIYVDKNND
                     GSGTLKRYKKKSFDWYKNVIKFNGEIL"
     gene            complement(21707..23548)
                     /locus_tag="PC523_001742"
     CDS             complement(21707..23548)
                     /locus_tag="PC523_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2475269.1"
                     /translation="MKFERESEEIIKYVGGKENIISLVHCATRLRFKLKDTSKTNKEE
                     LLKMKDVLSVVNSGGQYQVVIGNKVTDYFETITKKAVLQVQNTGEQGEKVSIVSRIFE
                     SISGAFSPLIPALAGAGMIKALLTILTIMKWMSDTSSTYAILSAAGNAVFYFLPIFLG
                     ITLAIKLGANPYVGGVIGAALLDPNFTALIGAKGGANFFGMPVTAIDYSFGVFPIFVA
                     IFIYYYVDKLLKKIILKDLQLFLVPMISLLVMVPLTVILFGPIATNVGDVISNIFMGL
                     FEFNRVIAGAAIGIAMPFLAVLGLHWGFTPITLQNLATMGGDPIEGAAVAAVFAQIGI
                     AIGLFMKVRKSKKNSELKVLAGSTAVTGILAGVTEPIIYGIILRYRRMIPVLAIAGGI
                     GGGICGVFGVTCNAYVFHNIFSMPVYTPFLGYILGIGTALVLGAVLSYFFGLSKEECE
                     EGNEEVAFDEDDGIEEILSPIEGLQIELSEVEDEVFASGTIGNGMAIIPSKGEVVSPV
                     DGTITTIFPTKHAIGITSDKGVEILIHVGLNTTMLDGKYYITHMNAGDKVKKGDKIVS
                     FDKDKIEGEGYSIVTPIILTNTNDLADVIVKENKHIKIGTPVIKVVY"
     gene            complement(23674..24507)
                     /locus_tag="PC523_001743"
     CDS             complement(23674..24507)
                     /locus_tag="PC523_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2475270.1"
                     /translation="MVIKKVFNNNVVFARDNSGQEVILMGKGIGFNKFTKDLVEIDKI
                     EKKFIFNNEESLSELHSLLDRIPLKDVELVSEIIQMGRDELGDNLNDSILITLSEHIS
                     YMLKRMDEGLVFQSPLQWEIRQIYPNEYKFSKKAVEYLRKKLDKDIPDSEIAFITIHF
                     ANAQLETKDMQETLLLTKIIDNVLDIIKYHYGIEINEYSFDYTRFISHLRCFIKRQLS
                     GENANVDTSLLDIIKMKYKSDYNCAKKVKNFLEKTYKWKISENELLYLTLHLNRLSNI
                     K"
     gene            complement(25073..25318)
                     /locus_tag="PC523_001744"
                     /pseudo
     CDS             complement(25073..25318)
                     /locus_tag="PC523_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25545..25757)
                     /locus_tag="PC523_001745"
     CDS             complement(25545..25757)
                     /locus_tag="PC523_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2475271.1"
                     /translation="MAILMKTKIKEYREKFLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEQE"
     gene            complement(25758..26174)
                     /locus_tag="PC523_001746"
     CDS             complement(25758..26174)
                     /locus_tag="PC523_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475272.1"
                     /translation="MSKSETGVKSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNIDTGIILIAVLYIQSLSKLVLYIYFDKRGICN"
     gene            complement(27482..28111)
                     /locus_tag="PC523_001747"
     CDS             complement(27482..28111)
                     /locus_tag="PC523_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDF2475273.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKAKEALIQ
                     DSIVAAICGATLGLAKKGLLDFRNHTSNDLEFLKMVVPNYSGEKYYKMESVVSDEHLV
                     TAPGIAPLEFTFQVLKLLEVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(28190..29152)
                     /locus_tag="PC523_001748"
     CDS             complement(28190..29152)
                     /locus_tag="PC523_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF2475274.1"
                     /translation="MAKIDNMMSILWMLSSDKKITAKEIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDIGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQKQEDILTNHLIALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKAKSKYRIINPYGIVHWNNNWYVVAFCNMRNEIRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQSIIQLVVEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIQPLSLRKKMIEDLLELIDYYQI"
     gene            complement(29524..29962)
                     /locus_tag="PC523_001749"
                     /pseudo
     CDS             complement(29524..29962)
                     /locus_tag="PC523_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427593.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            complement(30877..34353)
                     /locus_tag="PC523_001750"
     CDS             complement(30877..34353)
                     /locus_tag="PC523_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475275.1"
                     /translation="MQQKCKKKALSMILSISMGLGSVASFNVSNTFADTIEESTNAGN
                     VEGDTNAGNVEGDTNAGNAEGDTNAGNAEGDTNAGNAEGDTNAGNAEGDTNAGNAEGD
                     TNVGNAEGDTNVGNAEGDTNAGNAEGDTNEGNAEGDTNEVLVEEDTNAEEYDSVGDFK
                     ISGGEINVDFKFESGVLTILSGKQMTIQNNNPNTPTTHRIVIPKDVKAELIFNGVNIK
                     TASNSPFTLTPDKDGGAYAHIILQDGSKNILKSGDQLYPGIRAGKSTTLIIDDSVVNR
                     DIEGEFIVPENGKIPRDVTLENGDKLKKGDRLTLLDSKNRGSLTAVGNSYAAGIGGGD
                     TENGGNIIINGGIINATAMEGYNTGFGAGIGGGSIGDGGDITINGGTVTAKGAYHGAG
                     IGGAYYISGNHTLSPSVVDRNKGGGYSGNITINGGLCYSYGGAHGSAFGDGCVNPGAN
                     RGYRVLVTGGTVLPYMTGAGDWIFDLSAITGDVIVTGGSLKATTFKSSGETVAYGDLE
                     KKTKVFMSKVSLKYLGQDKVNTKLVDDFTMQINGKEYPYGAPSYTDDQGVLYLWFSDK
                     FTGSEVSVKLAIVDKDTGDTLKSDTFYIKDIGPTTPFLKQYEEFIIDEINTPPGSINK
                     RYDGLPFGKEQEKAFLDWILKDGIPVTNPKGGKLTDGTYIIISSQRLNEETMEPDTSL
                     GSDDLTKQVDVGKYQLIITSKQYASVAPFNESYWGHRSHYNYAEITPADTETSLVVET
                     PKVKTDVFKPTDTFTLNATVSPDKKEGPDCASPKGYVQFYINGKEYKDPVELKTHDKV
                     SESHNYSTASIEWKPLDSNHHILNTEQTITVKYIGDDINYTKDSKDEKKLKLDITNVD
                     IDGDGIPDINIDTDGDGRPDINIDTSGDWKPDINIDTDNTGEWKPSTEGGNRDGIWKP
                     DKNIDTNGDGNPDTDYNRPAIDTDNDGVDDYWKPDKNVDTDSGGYDTGNPDPNKPDPD
                     NPNPDNGGNSKPDSGNGGNSKPDSDNGGNSKPDIDTDGDGKPDIDGDGKPDIDIDTDG
                     DGKPDINIDTDGDGKPDINIDIDGDGKPDINIDIDGDGKPDINIDIDGDGKPDINIDI
                     DGDGKSDVNIDIDGDGKPDINIDTDESLDSNAGQNVQTGDQSNIMLDLALMFTSLFFL
                     IKNLINKYLRRK"
BASE COUNT        11231 a         6165 c         3578 g        13585 t
ORIGIN      
        1 tttctttttt atatatagct ttatttattt ttattactca taatttacaa gttcttatta
       61 aaacatagtt atattaatta attcaaatta aatatatcgt gttataataa gtatgtaatt
      121 aaagaaagag gtgtatttat gcgaacttta aagtatgcaa ttttaggatt attaaataga
      181 aatcctatga ctggttatga tatcgtaaaa gagttcaatt ttcaattagc tgagttttgg
      241 aatgcaaagc atagtcaaat atatcctgaa ctaaaaaagc tggtaaatga aaagcttatt
      301 gtctatgata ttaaaattag tggtgatgta ttagaaaaaa agttatacac aatcacagaa
      361 aaaggtaaag atgagttctt gaaatggctt gaaaaggatg agccaatgga aagaactcct
      421 aaggatattt ttcgtcttag gatgtacttt tctaataatt tagatataga tactaggctt
      481 attttactag agcatcagtt gcttcaacac caagaaagaa ttgcgttctt atacagtcaa
      541 atggaacgtt atagtgaagt tcctcctctt aatagtaatg attttggtga ttatatagtt
      601 cttgatggag ctattatcag agaacaaggt gctataaaat ggctaacaaa ttgtattaac
      661 catcataaaa atttggataa tactactttg aaataataac ttgttgattt taatagtgaa
      721 tttaattata gatattaaat ttataaaaaa gactgtacac aactttgtct ttgtatacag
      781 tcttctatgg ttgattattc aaaatagcta attataaaag ttaagtattt tttattaagc
      841 tattatttta tcttcttatt acctttcaaa atctccttta tatttacaat ctttcacagt
      901 cgaattgcta tctcttctca taatacaaat ccagttgatg tattgttact aagtttaatg
      961 gtaatcaaat tctttaccaa taagtataaa aattactaat gtgttataat tttcttaact
     1021 actaaatttt acatgcattt gaatagaata tgtcaataaa ttactataat aaataaaaaa
     1081 ttaagagtta aaataaaaaa tagttaggga gatattatta tgattttata ttttagcggt
     1141 actggtaata gccgatatgt agctcgtaaa attgcacaag aattaaatga tgaacttata
     1201 tcactaaatc aacttataaa agaagaaaaa acagatgaat taatatcagc agataaacca
     1261 ttcatattcg tatgtcctac ttatgcatgg agattgccaa tagtggttac ggattttata
     1321 aaaaaaacaa aatttctagg aagcaacaga gtttactttg taatgacttg tggaggagat
     1381 acagcaaaat ctataaacta tattcaaaaa ctatgtaaat ataaggaatg gcaattaaaa
     1441 ggtatggcag aaattaaaat gccagagaat tatatagctt tattttctac aacagataaa
     1501 gaaacatcaa aacaaatgat taaagaagca gataaacaaa tatataggat tatttctgat
     1561 atacgcaatg aaaatgaatt tgaaactatt actcctagtg gcttaggagg tgcaattaaa
     1621 agtggtataa taaatacagt tttttataag acaattatta gtgccaaagg atttcattat
     1681 actgacaaat gcattggctg tggaaaatgt gtagagctat gtcctttaaa taatattaat
     1741 ttaaaaaaca aaaagccagt ttggaaaaat aactgcaccc attgtatggc atgtatttgt
     1801 ggttgcccca ctgaagctat tgagtataaa aataaaactc aaaatagaga aagatactat
     1861 ttagaataat atattcttaa tatttaaacc attataaaat gaactatacc cattgtttta
     1921 tacagtttta catattttaa ctgtctcatg ggtatagttc aaaagatagt gtctcaaagt
     1981 gaacttttta gttcctaaat cactcttttt tattaaatca agtatacttt aatcaccttt
     2041 tcttatatca ttttcactaa tatgttgtat tccaacaaat ataagattta ttagctctgt
     2101 tagtgacttc cattgtgatt tttttgaatc aagataataa tagtttttcg ttccttctct
     2161 acgcacatta acaatctctg cttcttttag tatcttaaga tgatgagata cggcaggacg
     2221 agaaagatgt gttttttctg tgatttctcc cactcgtata ccattaaaat cactctctaa
     2281 gagagtaatt aaaatcaact gtctagtctc atcacccata gcagagatag ctttgcgaca
     2341 ttctttaaat ttagacgtta tttcttgcat tctttgttga cattcttttt ccataaactc
     2401 caccctctct tataagtatc taacatttgc tgcttctttc tgtgcagtac gtctgtattt
     2461 aacatctggg tatccaatta ctagtgttgt aagaacatga ttactgcgtt ttagacctaa
     2521 aagctttttt aactttggcg aattatttgc tacatctgaa aaatatccac taaataaaac
     2581 tcctagacca taagactctg ccattagtga catatttgat gctgcaagtg aaccactaat
     2641 cttatctttt gtaattatca ttattgcaat aggagcattc ttaaagaaaa atttatcatc
     2701 aatctcaact tcctttgaat atttaatagc tatatttgca ataggtttta tttttctaaa
     2761 aaatttcact gcttcgtttt cataaattga ctttttattg tctaaaacaa tatatgatac
     2821 atcttgtgaa tttttagcac taggggtata cctacctgcc tcaataattt gctttacaat
     2881 ttctaaagaa acttctttgt ctttaaattt acggatactt cttctagatt taattgccat
     2941 taataattca tctgaatcta gatttggctt atttgtaagt tctattggag gttcatcaaa
     3001 tcctgtcagt gtaatagctt tttggggaca gattgctgca caatgcccac acattagaca
     3061 atcttgtttt ttaattactg actttttatt ttctataatt atattgttta ctggacaatc
     3121 atttttacaa agcccacatc caatacaaag ctctttgtca acttttatta tatgttggta
     3181 tttcatattc attcatctac ctttcgttca ttggtttaaa ctattataac acatagatat
     3241 tttttatatt cattggtaaa gaatttgatt accattaaat ttttaatctc tctattacac
     3301 aaatttttca tagaatttta aaatatcata gtttatacat aataaaaaag acaagcttaa
     3361 aacctaagct cgcctttctc ataattttaa tacaaagtaa atttcactta cttcccatac
     3421 ttcctaacaa ttttattctt taaaatttta tttgtgaatc atactatcca taaagtacaa
     3481 cctaaaataa tcctgtgtta attttctata gttaaaaaaa gttaaaaatt atacttaatt
     3541 attaattttt atactcttct tatttaattt aatacaagtt tcttatttaa tataaatttc
     3601 ttgtctaata taaatcttca ccatttgaag caattacttt cttataccat tcaaagcttt
     3661 tctttttcgt tcttcttaaa cttccattac cactattatc tctgtctaca tatataaatc
     3721 cataacgctt ttccatttca cctgtaccag cacttactaa atcaataggt gcccatgtag
     3781 tataacctaa taaatcgaca ccatctatgt aaactgcatc atgcatagct tttatatgtt
     3841 ctcttagata atcaattctg taatcatctt ctacataacc attttcgtca gctatatctt
     3901 tagcacctaa tccattttct acaataaata gtggcttttc atatctgtca tatacatgat
     3961 ttaatgtaat tcttaaacct agtgggtcaa ttggccatcc ccattgacta tatgaaagat
     4021 atggattttt agcagatgta aatatgtttc cttcagccat atcacctgca ttttcatcag
     4081 ttctaacaca acggctattg taatatgaaa atgaaataaa atctactgta tatttattta
     4141 ataattcaat atcaccagat tccatttctg gtgcaatacc tttttgctcc atatacttta
     4201 atgcatagtt agggtattta cctttagatt gtacatccac aaagaagtaa ttttctctat
     4261 ctaactttgt agcttcccat acatcattag gattgcaact ataaggatat acacttccag
     4321 ctgcaaacat acatccaact aagttatttg aatcaatttc atgtgctatt ttagttgcaa
     4381 ttgcactagc tacaagttca tgatgagcag cttgatattt tatcttttca acatcttcac
     4441 cttcttcaaa gcaaatacca gcacccataa atggagcatg taaaatcata tttatttcat
     4501 taaaagttag ccagtacttc actaatcctt tatatcttct aaagataaca ttgcataatt
     4561 tctcatagaa acctatcatt ttacgattac gccatgcacc atacttttta actaaatgca
     4621 ttggacaatc aaagtgtgta atagtgacta atggctctat tttatatttt ttacattcct
     4681 taaatatatt ttcataaaat tgaagtccct tttcatttgg tgtttcttca tcacctcttg
     4741 gaaatattct actccatgca atgctcattc tatatacttt aaatcccatt tcagcaaata
     4801 actttatatc ttctttataa ttatggtaca tatcaatacc agttttagct ggataataat
     4861 gcttatcatc aaaatcgaac atttttagtt ttcctgtaat aactgctgtt ctatcttttc
     4921 ctgttgggca tacatctaca tttgccaatc ctctttcgtc ttcattatat ccaccttcac
     4981 attgattggc tgcagtagct cctccccata aaaaatcatt cttaaaactc atagtcaaag
     5041 cctcctaaaa ttataaccaa ttcactagct tatactaatg ccttaatttt tactaaaata
     5101 agaccgtaat tagattatcc tctaattcaa tattttttct tcctgtttct actacatcta
     5161 aaaattgtga tgaatttgta ataacaattg gagttacaac actatatcct aactctttta
     5221 ttgcatcaat gtcaaaattt aatatagttt gaccttttgt aattctatca ccttgtttta
     5281 tacatgcctc aaaacatctt ccctctaact gaatagtatt taatccaata tgaataagca
     5341 cttcaacacc tgcatctgaa agaatcccta tagcatgtag actaggaaat aaggttgtaa
     5401 ctactccatc tactggagca actacttttc catcactagg tattattgct accccttgac
     5461 ctagtacacc agatgcaaat gctgcatcct ctatctcact taattttaat acttcacctt
     5521 ttatagggct tgctactatt tctctttcta atttagtaga cttgacttct tttatttctt
     5581 ctttcccctt atttcttact tttttagact cttctacttt ctcatcttta tataaaatca
     5641 tagttaatac aaaagctacg accattgata tagctatacc agaaagagct actattaagt
     5701 tacccattcc tccatctggt tcaatcattg ctggaagttc aaatattccc atacctccag
     5761 ttataaactt tctaagatta aagaatccat aaaaagctcc tccaatacca ctagcaatac
     5821 aacttattat aaatggtttc tttaaaggta ataatattcc ataaattgct ggctctgtaa
     5881 ctccaaatat accagatata aagtttggta ttgccatctc ttttagtttt ttatctttag
     5941 tcttaaagaa tattgccaac acaactgctg aacttgcaaa tgtacaagca aagaatggca
     6001 tcataacatt atcatatcct aaagtcataa cattgtttat atatacagga ataaatcccc
     6061 agtgcatacc aaaaataaca agaatttgcc acgtaaatcc tactatagca cctgctaata
     6121 gtggactaaa atctctgatt gaaattacaa cttgtgaaat aactgtagaa ccaaatgttg
     6181 ctataggacc cagaacgata agtgcaacag gcactgttat taataatgta agcattggca
     6241 caaagaaaaa cttaactaaa tctggaataa acttattaaa taatttttca cacctagatg
     6301 caaaatacac tataaatata attggtacaa ctgttccagt ataatcaatt gatacaattg
     6361 gtagaccaaa gaaatcaata tatactggtg atgcaaacat tgttccttta aataaagtgt
     6421 acatcacatc tgctcctgct gatatactgc taccttgtat agcaggataa catactgcag
     6481 ctcctagtgc taatcccaac attggtttta atccaaactt cttagctgaa gtatatccta
     6541 aaaacaaagg aaggaatgta aataatgcat ctcctgctgc atttattacc atataaccac
     6601 cagatacatc actatataac cccattgcta caaataatgc attaaatcct ttcaacatac
     6661 cacaagctgc cattattcct aatactggct gaaaaattcc tgatattata tctataccct
     6721 tatcgagtat actacctgat tttttagttt ctgaatttgc acttgagtta tctaaaccaa
     6781 ttatttcaac tacatcttta tagacagcct caacatgatt tcctataact acttgatatt
     6841 gtcctccact tttcattaca gtaacaatac cttccatatt ttttaataca ttgtcatttg
     6901 ctatactttc atcttttaaa gtaaaacgta atcttgtaat gcagtgtact aatccactaa
     6961 tattttcttt tccaccaaca ttttttacta tctcttgtgc taattgtttg tattttccca
     7021 tttttctcct ccatatttcg tttaaattaa tttttgaaat ttgaaggttt ggccaactca
     7081 atgttaccat ccaaccatag gtataatttt gttaatatca ctcctttcaa taacatttac
     7141 tatatactac tgaaaattaa tttacttata tatttgaatc tttaactact tttgctaaat
     7201 gtattgttaa atatataagc tcatcatctg ttaatatgta gttatattgt tttttaataa
     7261 aatccttaat cttaactaca cattcaaact ctttattata tttagattta ataatactta
     7321 ataattcttt atctgtccca ccttcatgtg cattatttaa aagtaatctc tgtgcaaaaa
     7381 atttcaaatg cattataaat ctataataaa acactgattc ttcatgaaat tctatcctaa
     7441 aatgatacct aactatacta agtacttctt gtattaactt tgtaatatca gatgcaaaag
     7501 tatgcccctc acttagctga gcattcacta tatgaaaagc tataaatcca gcctcatctt
     7561 caggtaattt tacgcctgtt ttattttcaa taatatctag tgccttcatt ccaatttcaa
     7621 actcttcttt ataaaatctt ttaatttccc aaaggattgc attttttaca tttattcctt
     7681 ctttcattct ttgaattgca gaatatgtat ggtcacttaa ggatatgtaa atgctttcat
     7741 ttagcttctt gtttaacttt atttgtgcat actctataat ttcacttgat aatttcatgt
     7801 gctctatagg aatattattg agtaattctt gtagtttgtt tgatacttct ttatttgata
     7861 ttctaaatac tttgtttact ttagttttat ctattagatt gccttttgat ttttgataac
     7921 caattccttt acccatgacg ataatttctt catcattatc atctaatgtg attacaacat
     7981 tattgtttaa tatcttatta attatcatga ttatcctcct tttaagcata aaaaaaactt
     8041 atccataata atgggcatac tccttcctag gatactgcta ttattaatga ataagcttag
     8101 cttgtactta aacaattact acctaattcc aattactatg ataaaacatc atacatattt
     8161 tgtcaacttt ttatttccat ataaatagat ttactacacc ttctgttata agtaatattt
     8221 ctatttcttt atgccagtgt ggtgttgtta atacttctcc ttcatttgat agactacgaa
     8281 aaggaaattc atgtattaat ttaggcattt ctaaatatat tgccatatat atcctctcct
     8341 ttctagtgtc taaaaattaa tatatactat ttaaaataat acatacttta ttttaaaaac
     8401 ctcaaaatta taatttatac aatacctaca cttcataaaa tatatctaat atttcttttt
     8461 ttgtatttgc atttaaaagt ctttcactta tatctttacc tcctaaaata tacgctattt
     8521 tagatattaa tgtcatatct tcattgtctc cagattgtgg catagcaaac ataattatta
     8581 cctttacagg taagccatct atacttggcc aatctatacc ttttttagat ttacctatta
     8641 ccacagatgc cttctttaca tattttgttt ttccatgtgg catggccatt tgataaccta
     8701 tactagtaga gcctattttt tctctacgtt ttagtgcctt tataaactta ctttttaaat
     8761 ctatacttcc tgtatcgtat aatttatcta ccaactcttg tatgatacct tcttttgtag
     8821 ttgatttcaa atctaaaata atactttctt cgcttaatat ttctctaatt tccatttgtt
     8881 ttctccagta gattattttt aaataatatt ttaatatcct ctttgctctt gcaatctatt
     8941 atatagttta tataactctt ttgcctatat aaatttgata tttccattaa aatattcttt
     9001 aaaaaacttt gattactagt ttctaaataa atatcaattg ttctattaat attattgaca
     9061 ttcattatcc ctttatttag tttactttga ttatgtatat atatatttaa atcctttcct
     9121 aatgaaatag atttatattt actccctaaa tccacgccta ttaaactttc catctgcatt
     9181 acatcaaaat taaaatatat attatcttct aaatacattc ctaataagtt tgtatctaag
     9241 ttcatacttt tcttaggttt agaaagtaag gcatttttta tattactaat atcactttcc
     9301 attaatagta ctgatacaac tatatgtaat ttatctttca agtctaggtt tacagtagat
     9361 attacaaagt ctacatcatc taaatttcga ttgctcaact gatgcatagg aactatatca
     9421 actatagtcc attcaggaaa ttctcttttt aatctggctg caagtagctg agatgttcca
     9481 taacctgtat gacaaactac tattactctt ttactactca tatttctctc tattgctgct
     9541 tgaaaatatg tggcaagata cgaaacttca tctatactaa tgttcttcag attatcatac
     9601 tctgtcataa tatttatagt catcatacat aatcccaaca catctgaaaa tcgctcttgt
     9661 atctctccta ataatggatt ttttatttgg atatcatatc ttaatctgtt taacataggt
     9721 cttacatgag atgctagact tttttgtagt aagtcatcat ttttgagatt tatatttaaa
     9781 aattgcgaca tattctctat cattatagat gaaaactttt cactttcact taatctatat
     9841 gattcatcat tgtaactatt attactttcg ctactaaatc cagatgacac taaaaaaata
     9901 tatatgtaca taatttcatc tttcgttatt tttactccgt atctattttc tattttgcct
     9961 ataagtgcag tgacattttg atatactaat tcatccaaat tatctacaga aacttctctt
    10021 tcttcctgag actctatttg attaccttct tcgattctct ttaaacatat aagtatatgg
    10081 gtaattaaat ttatatagta gggctgactt atagtgtatc ctaaattgta ttctaactct
    10141 gtaattatag tttctacaaa tataatagaa tcaatatcaa ataaatttat caacgcacta
    10201 aatgtagcag aatctaatct tgttaactta gatatatcat gctgttcata attttcatct
    10261 aataaatcat ccatcatttt ggcaatcgat ttccttatat ccacttcttt acctataatc
    10321 ttagttccct ctaaagtttt attaagtttc aaattgtatt cttttatcca tgcctctata
    10381 tatttaaaat catttactat agaagtttta ctcacataat atttatctgc taactgattt
    10441 attgaagtta tattatttga atttagtaat aaatacttta aaatctccat tcttctctgt
    10501 tctactgata aagatttatt ttccttcgat tgtatattta aactattaat taagtctata
    10561 ttcttctttg ccaaagattc tattaatatt ccatatcctc tttttatatc aatttttata
    10621 ttaaaagttt ctaagtattt ctcaataatc ttcaaatctt tttgtaaagt tttatcggat
    10681 acatttaatt tatctttaaa gtattttata ggtctatatt tattttcgtt taatatcaat
    10741 ttaagaaatt caatttgcct cgacacaagc atacttatcc ctccctacct atgtttatat
    10801 atttctatgt tcatattaag tactatttta acatatttac taactatgta aaattactaa
    10861 gatacttctg tatcataaaa gaatttatga tacaccttat taaattgata taatagaaat
    10921 ataatttcaa aataaaaaat aaggatattt agaagtagtt aactaattct aaatatcctt
    10981 atttttattc tatgtacttg tggttattct attacttatt tttactatca tttataattt
    11041 aagccccaag tacttcaact tcactatttt cactccttat agtaggcttt gttaccccta
    11101 taatcaatgc tgttattact gttccaatca atatacaagc tatatatgca actggtgaac
    11161 tacatgcaaa tggtattaca aatattcccc catgtggtgc cattaaagtt atatctaaac
    11221 tcatacatat tgccccacct atacaagaac caattatatt tgctggaatc attcttaatg
    11281 ggtctgcagc tgcaaacggt attgctcctt ctgttatgaa agatgctccc attatagcag
    11341 ttggtagaga tgactttctt tcttctttag taaacttatt tttgaatatc aacatagcta
    11401 atgatatcgc taatggaggt accattcctc ctaccattaa agctgctgat ggttcaaata
    11461 tattacttga aaacatagcc aatccaaaag ctgaagccgt tttgttaata ggccctccca
    11521 tatctgatgc catcattcct gctaatattg cacctaataa tactttatta gttccagaca
    11581 tgctttgtaa ccaagattgt aaagcttcat ttaaccatga tgctggtgta ttaactacaa
    11641 agaacattat agctccactt gtaaatgcaa tcaacagtgg acatatcaat acagcattta
    11701 ctccttttaa tacatctggt aaattttctg ttatcttcat tactcccaag gctatatatc
    11761 ctgttgaaaa acctgccacc ataccaccta taaaccctga acctccaata actgccataa
    11821 aaccagctat catagcagga gcaaaacctt gtcttcctgc tattgattta gctataaaac
    11881 cagcaagtat aggtataaat aacttaaatg ctgaatctcc tccaagttgt ccaaagaatc
    11941 cagctatagg gttgtatgtt tcatgagttg ggtctgatgc aaatatccca aacataaatg
    12001 ctatggctat accaattcca cctattacta caaatggaat catttcggat acaccactca
    12061 ttaagtgctt ataaacacct tttttctctg acccttggag tatttctttt gaatcttcta
    12121 aagttcttcc acctggttta atttctttag ccattccatt ttcagcattt ataattaaat
    12181 ttttagctaa tgaaacattc ttagcatctg ctactttctt ttgtaacact ttctttccta
    12241 taaatctact catttgaact tctttatcac aagctattat tacgtattca gctcttttta
    12301 tatcttcttt agtcaactta ttttctacac ctatcgctcc atgagtttct atcttaactt
    12361 catatccaag ttcttgcccc gcttcctcta atgctttttt tgccataaaa gtgtgagcta
    12421 tccctgttgg gcatcctgtt attccaacta taagaccttt attttgatta tcacttgaat
    12481 ctcctgattc tacagcttta ttattaaata tacttaatat ttcatatgga gtttctgcac
    12541 ttagtaggtg cttaagaaga ttttcatcca ttagatatgt agctagttta gatataatct
    12601 taagatgttc atctgagcct gactctggaa ctgctatcat aaaaaatgac ctaagttttt
    12661 gaccatcatt atccgtaatt tccttatcag ttcttaaata catcattaca ggttgattta
    12721 ctacatttga cttagcatga ggtatagcta ttaaattacc catattagtt gatgattctt
    12781 tttcacgttt attaatatca actataaact tttctttatc atttatataa ttcccatggt
    12841 acaaatgatt tgccatataa tcaatcacat ctttttttgt ttgtaattgt ttatctagta
    12901 caatacaatc gcttcgtagt atttgaccta tcataaattc tcccctcctt tttatttgtt
    12961 accttataat taattcttgt ttttataaaa gcactttaat ttatctatgg tattgaatag
    13021 attcatttta aactctataa cttctcttgc agcatctata catggtggat atatagtgtc
    13081 tggctcatat aagcttgaat ttttttctaa tacttctctg cacttcttat aatatgccga
    13141 cttaacatca cttgaaatat ttattttaca aactcctagt ttaactgctt gtgctatttc
    13201 ttcatctgga tttgatgaac caccatggag aactaaaggt atatctacta cttctcttat
    13261 ttcttttaat aaatctagtt taagctctgg tttaaaacct tttggatata taccatgtgc
    13321 tgtaccaatt gcaatagcta gtgtgtctac tcctgttttt tcaataaaat ctttagctaa
    13381 ttttgaatct gtatatatta tttcatcagt accaccttca cttgatgttc ctgttgttcc
    13441 tattgttcca agctctcctt ctacagatac atttaatcct tttgctattt ctactacttc
    13501 cctagatata gctacattct cttcatatgg taaatgtgag ccatcaatca ttactgatgt
    13561 aaatccacat cttattgctc ttaatatttg ttctttattt tccccatgat ctaagtgtat
    13621 taccataggt actttgctct tatttgcttc ttctatacaa ctagctacaa agctatcccc
    13681 taggaagctt agctcatttg ggtgtattgc aaggattact ggtgcatttt tttcgttagc
    13741 actctgtact actgctttaa gtatttgccc acttcctata ttaaatgctg gcacagcaaa
    13801 ttgattttct tgtgccactt ttagcatttc cttcatattt attaacatat tatacctccc
    13861 gatattttaa attttaggtt gaatttgatt cttgttaatt ttgttgttgt ttatatttaa
    13921 ataatatcag ggtaatgacg gttcgaaaat aacaatcgtt ttccttagtg aaaaggaact
    13981 tttattatac actcttagtt ccttttcact aaataatttt tagaagtctt tatcatagtt
    14041 aattatacct ctcatgtaca tcttttcgtc ttaagcgttt taatttaata ggctttaacc
    14101 ttactatatt tttataataa acaattaaga tactttaact ataaatttaa agatactcat
    14161 gcaatagtta atgttatatt agagtcaaaa aaattaaaaa gatattagct atttagccaa
    14221 taccttttcg tggatttcaa tagtatatac gatttatatg aatcatactc acgcttatct
    14281 aatgttttac ttccataaac tttacacaaa acaccaaaat gacaccctcc aaattcatct
    14341 ctagctaata aagtgatttg gttttctaag caccaattta taaattaacc tttattatct
    14401 tatgtaaatc tttttctatc taatttaaaa attattccta atgctatata gcatatacca
    14461 attactaata caccataagc catagatact cctatcttaa attcttgcat attagttaca
    14521 atagaaattg aaataggtct attataaaca ccataaagca aggctgacat tgtatattcc
    14581 ccaattgtac gaatgaatac taatatagct cctgaaatta ttcctggcat catatttggt
    14641 actattacat tataaaaggt ttttatcctt ccagcaccta aactccttga tgattcttct
    14701 agtcctaaat ttattgactt catagcaacc tcattggatg aaaataataa tggtagggca
    14761 taaattgtat atgctatagg tacaatataa aaacctccta ttaaggattc attaaaagca
    14821 aatatacttt tttgattaaa tgtattaatt aggttaactg caatggcgct tgcaggcata
    14881 ctccaaggta gcattataat aaattttgct agtcgattat atgtagtatc cttcttaatt
    14941 actaaatacg atactggtat agtgattatc aatcctatta ctactgccat taaagacatt
    15001 ttcaagctgt ttaatatagg ctgtaacaca cgtgattttt ggaaaatcat ctgatagttt
    15061 tccagtgtaa agtctctagg aaatatagtt gtcataattg aatgtgtgct tacaaaggat
    15121 aaatacacaa tagtaactat aggaagcatg atcataacaa tttgtatttc aataataacc
    15181 ttacatacaa atgtaaataa gtcttttttt ctattatgaa ctttataacc ttgagacttt
    15241 aaggatgcta ctactccata tttttttcca tagtactgta ataaaatcat aaatgaaatc
    15301 cccattagta acaaaataat tacttgaact gaagctatat ccatgtggtt attagcctta
    15361 gacctaacaa tctgagtgct taaaacttta aaaccaccac ctataagatt tggtgctgag
    15421 aaagaactaa tacctgatgc aaaagttata atagtagagg ttataatggc aggtgtaata
    15481 acaggccata ttacatcaaa aaatactctt atctttgatg ctcccattcc ttttgcagct
    15541 tcaactgttg aataatccac atattttaag gcaacatata catttaaata aaaatacaca
    15601 tactgagtat aggcaaccac aaataaaact ccctttaatc cactaaaatc ataaggtttt
    15661 ttgataaagg atagtaaaat ttctatagat tttgtgacta tcccactttc tccataaagc
    15721 tgaatacatg caattaccat aattactcca ggaatcatca ttggacttag taaaagaata
    15781 tgtatgattt tcttgtgttt atcacaaaca aatgtcatat ataaggcaag tgatattccc
    15841 ataattccac atacgagtac agaaccaaat cccaaaatta tagtattgct tactaccttt
    15901 aaattattag tatttgaaaa atattcttta aaaacttcaa aagaatatcc acctttacct
    15961 tgaaatgctt gtgtaaaggt attgcctatt ggtatgataa tatatccaat caaaatccaa
    16021 aataatatac tgtataaaat aatcgaaata acatttttag gtgtaaattt actacgtttt
    16081 atttttttaa tcatattagt taccttgtag cacacctaaa tatgccatat cccctacgtt
    16141 taatttattg ctattattca gtctattaag ttcagttact tgatattttt ttccattatt
    16201 tgatactgta tattctaaaa ttgctcctaa gaaattctta gattctactt ttacttttat
    16261 aaaaccactt tggttggtat tttgcaataa taccatttct tcaggtcttt tataaatata
    16321 aggctttgtc tctttttcaa tattaaaatt aacataatct tctttttcaa tacagtttga
    16381 agtacctaca aagtttgcta caaagtcatt caatggtttt ttgtaaactt ccattggaac
    16441 accaatttga agaaccttcc ccttatccat tacagctatt ctatcagaaa tagctaaggc
    16501 ttcctgttga tcatgagtaa taaataaagt agtaatacct atcttttgtt gtatctctct
    16561 tatttcttcc ctcatcttga ttcttaaagc tatatctaga ttagacattg gctcatctaa
    16621 taaaagcata gtaggttcag tcgctaatga ccttgctact gccacccttt gttgctgtcc
    16681 accagataac tcatcaatct tttttttcct aaaatctttc attcctgcca gctctaaata
    16741 attatcacat ttttccttta taattttttt agacagtttt tttattttca atccataaga
    16801 tacattttct tcaactgtca tatttggaaa cagtgcatag ttttgaaaaa cagttcccat
    16861 atttctattt tctggttcta aattggtaat atttttatca ccaacataaa tacttccaga
    16921 agaaggtttt ataaatccac caattattct taaaatagtc gtttttccac atccagaagg
    16981 acctagtaaa gtaaaaaact ctccttcctt aatagatata ttaatatcat ataatacttt
    17041 ttcttttcca taataatgat taatttcttt taattctata ttcttcatat cgtctccaca
    17101 aattatctaa tatttataaa tctccaaata tacacatgac gaatcatcat agattttaaa
    17161 tctaggaaac tctattgcca tttcatcatc cttttctagt tccctaacct ttctatatat
    17221 ataatctata cctttaatct ctgctatttt catcatttca tttttatcaa aaatcttata
    17281 tctatcatat ccacatgaaa aaccatcact agccatcata attttcattt tatattctat
    17341 atcaataaat ccacttatac aattatcaac agcctcttta gaaaattcca aagcccaata
    17401 tccacctaca gtatttttct taagtctatt ctctattata atcggcatta cctcggcttt
    17461 tctctccttt aaagtaaggt ttgtaacatc tttggcttct tgcataaaat caaatatttt
    17521 tttatcaagt ctacataaag tctcatcttt taaaattttc acagattcat tatggttaac
    17581 taataaagta caatctccta aaagtaaata ctctatctta tctttataaa actttaatat
    17641 agaacatgaa caagaaggaa tatctattgc ttctacttct ttatcatatg ttaatttgta
    17701 gtactctttt gttatctcct caatgccttc tttaataatc acttttaagc tatcttctct
    17761 ttccatattc tcatgaaggt aactattcca ccaacttaca taccatttag catcactttc
    17821 tgtagagact aaattcttat tatttaaacc tgtagctcca tcaagtaccc atgccccata
    17881 aggtgataaa cctacgatat cttcgttaaa atcgccatta cctttattac ataataattt
    17941 ataatcacta atttttagtc ccatctttat ctccttgccc acataaattg tacttttact
    18001 ataattaaaa gttttacata ataaaaaatt tactttattt aagaaactat gtctacaaat
    18061 cactttatat agtcaattac ttcgttagtt gctcaaaaac tattttattt aataaatatg
    18121 tattagtatc atttgttgta tgaattataa aaaattatga gatgctatag ttcccttata
    18181 agtaaaatag cgaggaaaat cctcactatt tttgtatata atattaaatt atttaattaa
    18241 gctattttat agttttatta ttatctatta tatctgtttc ccacttttca atccagttag
    18301 actgattttt tccaattaca gaccaatcta catccatagc tttatattct tttgacatcc
    18361 actttggtga atctttaagt gcagaatcta atgttggaat acgattaaaa tcatttgcaa
    18421 ccatagcttg aaccttagca cttccaacaa aatctacaaa tttagctgct gcatttggat
    18481 gtggtgcatt ttttagggct gctacacagt ctgtgattac tacagaacca ctctctgcat
    18541 ctacaacttc taatggcata ttattttttt ctttattctc tattatatca cttaatactg
    18601 caacagatat tgcagcttca cctttaccta ttgctgaata catgacactt ccactattgt
    18661 agtaattctt tatattttta tttaaatcct caagatactt ccaagcttcc tcttcactct
    18721 tagtatttga gtagtaattg attaagttac taatagtaga acgcatagat gatgataatg
    18781 agtctctagt aactattaaa tctttgtatt cttcttttgt taaatcgctc caatcttttg
    18841 gcactttatc agctgataat agttttgtgt tgtaaaaaat cattactggt gtttttatag
    18901 ttccatacca atatccatct gagtctttgt aagacttgtc taattcatta ttccagcttg
    18961 gtttagtctt ttcatataat ccttcttctt ttaattgcat gtatgtagat gtatctccac
    19021 caaacattat atctgcttgt ggattatctt tttcacttct aacacggtct gctaattcac
    19081 cttttaagtt tataaattca acattaactc ctgtttcctt tgtaaattct tcagaaattg
    19141 ctcctagcat atcctctcca tgtgtagtat atactactaa tgtatcctct aatttatcac
    19201 tttttgatgt gttatcattg ctacaagcta caagtgataa taacattgct acagttaata
    19261 aaacaactgc tatttttctc ttcatattga ttcctccccc atattctcca tgttcatttt
    19321 aaatgttatt attcttgtag tcaatgattc aacaacaatc tatataattg tgtttttcta
    19381 tagtcactta ttttattatt gtttttttct ataattatac ctatttgtat gcatataatt
    19441 tttatattca ccttaatcca ttttttagta tgagtatttt tctatttaaa acacacttta
    19501 aatattaata ttactatcat atcattattt aaattgtacc catcttttaa cagataacta
    19561 gataaaaata aatattgctt tatacttata tcctttgatg tatcaatatt tgacaaatca
    19621 gctttcccat tgctaataat ccctttgtat attaattcat ctcattaatt taatatgtta
    19681 tctttatttc tctataattc caagtcttta catacattat aagctacatt tttaatctat
    19741 ataatattct tatctcatta cttttatttt ctctaaataa atgtatagct ccttatatat
    19801 aatctaaatg tatatgtata gatacaataa gatataaaga ttttttattc ttattcaatt
    19861 ccactaattt agatttagta tgcttataat aagtatcttt catttcacag tctataaata
    19921 tattagtttc ttcaccatta ataattacta aaaaactatc tccacaataa tcttaaaaca
    19981 ttgtaatttt atactagttc atatattaat tttctctaaa agctaaataa atttgtataa
    20041 tattctatat attaaatcat atgtatccca atttttaact ttacctcttc aaatttatat
    20101 tcatttttaa taaaaaaata ttgactattt agtcaatatc ttttagtaga tttaaatggt
    20161 aaagactatc ctgagaatca tacccacgat tttaatctaa tttttaatgg tttccttagc
    20221 ataatcttat aaatattaaa atttatctac ttataaaatt tctccattaa attttattac
    20281 atttttatac caatcaaaag acttcttttt atatctttta agagtcccac ttccatcatt
    20341 atttttatct acatatataa atccatatct ttttttcatt tctcctgttg taaatgacac
    20401 tgggtctata catccccaaa cagtataccc tataacatca actccatcaa tttcaatggc
    20461 ctttttcatt tcccttatat gactagccaa atactctatt ctataaccat catccacagt
    20521 attatctgat tttaatacat cctctgctcc aaatccattt tcaacaataa atataggaag
    20581 ttcatactta tcatataatt gattaaggta aaatcttaga cctacagggt ctatattcca
    20641 cccccagtca gaagttttta agtatggatt ttcaactaca tcagcctttc ctgccgagtc
    20701 attacctgtt gttttcatag ttttatcagc tgatactaca gtagttaaat aataactaca
    20761 tccaataaaa tcaactgttc cttcttttag gattttttta tcctcatctg taatatctaa
    20821 attataattt tttctttccc ataattttag tgtatgcctt ggataatgac ctcttacatg
    20881 tacatcagta aacataagtt gttctctatt agattctaca aatttaatca tatcctctgg
    20941 attgcaagaa taaggataac ttggtatagc tgccaccata catccaattt taaactctgg
    21001 gtttatttca tgacctatct tcacaacctt tgcacttgct acaaattcat aatgaactgc
    21061 ttgatacata acttcttctt tgtcttcctg ctcttcatag attattcccg agttagtaaa
    21121 tgcataaata ggatttgtag ttatactttg attatttatt tcattgaaag tcatccagta
    21181 cttaacttta tccttgtatc tactcattac cacttcacag aagtttacaa agaaatctat
    21241 taattttcta ttcctccatc caccatagtt tttcactaaa tgataaggca tttcaaaatg
    21301 gcttaaagtt attactggct ctatattgta ttttaataat tcatcaaata aatcatcata
    21361 aaatttcaat ccatcttcat ttggaattga ttcatctcca tttggaaaaa tcctagtcca
    21421 agcaatactt gtcctaaaac acttatatcc catttcagca aataatctaa tatcttcttt
    21481 ataattttca taaaaattaa tggcttcatg attaggataa tttaagccct caattactcc
    21541 atctgttacc tttcttgaaa tagtatgagt acctgcagtc atcacatctg caatacttat
    21601 tccttttcct cctttattat atccaccttc aacttgatgg gcagcaactg cccctcccca
    21661 taaaaattct tgattagatt tcatagaaat atctcctcca gtataattaa taaacaactt
    21721 ttattacagg tgttccaatt ttaatatgtt tattttcttt aactattaca tctgctaaat
    21781 catttgtgtt ggttagtatt attggtgtaa caatactata cccttcacct tctattttat
    21841 ccttatcgaa agaaacaatc ttatctcctt ttttaacttt atctcctgca ttcatatgag
    21901 taatataata ctttccatct aacatagtag tgttaagacc tacgtgtatt aatatttcta
    21961 cacctttatc agaagtaatt cctattgcgt gttttgtagg aaaaatagtt gttattgttc
    22021 catctacagg agatacaact tctccttttg aaggtattat tgccatgcca ttacctatag
    22081 ttcctgatgc aaacacttca tcttctactt cagataactc tatttgtaag ccttctattg
    22141 gagataatat ttcctctata ccatcatctt catcaaaagc aacttcttca ttaccttctt
    22201 cacattcttc tttactcaat ccaaagaaat atgacaatac tgctcctaac actagtgctg
    22261 ttcctattcc taatatatat cctagaaatg gtgtataaac tggcattgag aatatattat
    22321 ggaatacata tgcattacaa gtaacgccaa atacgccaca tataccacca ccaatacctc
    22381 ctgcaatagc caatactgga atcatccttc tgtatcttaa tataattcca taaattatag
    22441 gttctgttac tcctgctaaa attcctgtaa ctgctgttga acctgctaat acttttaatt
    22501 ctgaattttt tttagatttt ctaaccttca taaataaacc tattgcaata cctatttgag
    22561 caaatactgc tgcaacagct gcaccttcta ttgggtctcc acccattgta gccaaatttt
    22621 gtaaagtaat tggcgtgaag ccccagtgta atcctaatac tgctaagaat ggcatagcta
    22681 tacctattgc agctcctgca attactctat taaattcaaa taatcccata aaaatatttg
    22741 atattacatc cccaacatta gttgctatcg gtccaaataa aattacagtt aaaggaacca
    22801 tgactaataa tgatatcatt ggcactaaga acaattgtaa atcttttaat ataatcttct
    22861 ttaaaagctt atcaacataa taataaataa atatagcaac aaatatagga aagacaccaa
    22921 aagaataatc aattgctgtt actggcattc caaaaaagtt agcaccacct tttgctccaa
    22981 ttaaagctgt aaaattagga tctaacaaag ctgctcctat cactccacca acatagggat
    23041 ttgcaccaag ctttatagct aaagtaatac ctaagaatat tggtaaaaag taaaatacag
    23101 catttccagc tgctgataat atagcataag tgctacttgt atcagacatc catttcataa
    23161 tagttaatat tgtaagcaat gccttaatca tacctgctcc agctagtgca ggtattaatg
    23221 gagaaaatgc acctgaaatt gattcaaata ttctagaaac tattgaaact ttttctccct
    23281 gttctccagt attttgaact tgtaaaacag ctttttttgt aatagtttca aagtaatcag
    23341 ttactttatt tcctattact acttggtatt gtcctccact atttactaca gatagtacat
    23401 ccttcatttt taataactct tctttgttag tttttgaagt atctttcaac ttaaaacgta
    23461 gcctagttgc acaatgaact aaactaataa tattttcttt tcctccgaca tattttataa
    23521 tctcttcaga ctctctttca aatttcatac aatctatcct ccattttcat aattttatgt
    23581 ataaaaatag acctaaatca atacaattag atgttaaaaa ctaattatac caacttaggt
    23641 catgcctgcc aaacagtaac aatcctacaa tatttacttt atatttgata atctatttaa
    23701 atgcaatgtt aagtacaaca attcattttc gctaattttc catttatacg tcttttcaag
    23761 aaagtttttt actttttttg cacaattgta atcactttta tatttcattt ttattatatc
    23821 taacaatgat gtgtctacat ttgcattctc accacttaat tgccttttta taaaacacct
    23881 taaatgactt ataaaccttg tgtagtcaaa tgaatattca tttatttcaa ttccatagtg
    23941 atactttatg atatctaata cattatcaat tatcttagtt aacaataaag tttcttgcat
    24001 atcttttgtt tctaactgag catttgcaaa gtgtatcgta ataaatgcta tttcagaatc
    24061 aggaatatct ttatctagtt tttttcttaa atattctaca gctttcttac taaatttata
    24121 ttcattagga tatatttgtc ttatttccca ttgaagtggg ctttgaaata ctaatccttc
    24181 atccattctt tttaacatgt aacttatatg ttctgataat gtgattaata tactatcatt
    24241 aagattatca cccaattcgt ctcttcccat ttgaataatt tcacttacaa gctcaacatc
    24301 ttttaaaggt atcctatcta gtagtgaatg taattcactt aacgattcct cattattgaa
    24361 tataaatttc ttttcaattt tatctatttc aactaaatct ttagtaaatt tattaaatcc
    24421 aattcctttt cccattaata ttacttcttg tcctgaatta tctctggcaa atacaacatt
    24481 attattaaat actttcttta taaccataaa ctacctccaa tgtaaacaaa aagagaccta
    24541 aaaaagacta ataatacttt aagcatttac tagaattttt aggtcacgcc tgattacttc
    24601 tatctcagta acgatccgtt tatttatctg tatgataact ttattttcat tataaattaa
    24661 taactgtact tagtcaatat ataattcatg tatatatcta cctatattat tttcttcaat
    24721 attcactcat tctacttttt ttcatataat ttatataacc taaacatgct aaaataagtt
    24781 ttgaagttat ttcataacaa aattaaactc atatattgtt tactatatat gctcgattat
    24841 ttatcaatat ataaggaaat cctttatttt tgtaactaca ataactgact aagttttgat
    24901 tttcttttat cttttacttc ttattattaa aaaataattc ttttactgtt gtatctaaag
    24961 ctttaaacag tttaatcatc aaatcattgt ttggattatt aaccttacct aacttactat
    25021 atagcttttt aactgcatat ctttaataga ccttttggta tgctaatcca ttttataata
    25081 ttaattcttg aatcaagcat atattaccct tacaatgcat atcttatacc ctctgtaaag
    25141 ttattcctga agaaattact cttacaagac atatctgata caacctcact tataagttta
    25201 tatgtttcag aagtatataa ataacccata cttccttcca aactcttacc tgttttctta
    25261 gaaaattcta atagatttcc ttacataaca attgtaacag agtcttatta gatttcataa
    25321 aactatacat cctctttaca tttaaacttt aaacttttat ctattgttta tcaatcaaat
    25381 gtatattcac tattttttat catttataat actcttcttc ttatcttaaa acaattataa
    25441 tctaacctta atcacatact atattatata aatattttta acaaacttaa taattaaata
    25501 actatatttt attatccatc ttataaatgt ttataaactt tccactattc ttgttcaata
    25561 aattcaaaca aattttctac tgtggtatcg aatactttag cgatatccat agctaacttc
    25621 aaagaaggat tatacttccc attttctaag tgtacaatag tctcacgtct taccccaact
    25681 aatttagcaa gttcattttg cgtcataagg aatttttctc tgtattcttt aatttttgtt
    25741 ttcatcaata ttgccactta gttacatatc ccccttttat caaagtatat atacaacact
    25801 aatttagata aagactgtat atataaaact gcaatgagga ttatacctgt atctatgttt
    25861 gatatattta tattacttgt ataaggtgtt gcaaagaaaa tagatattat tactatagaa
    25921 tatatcatta tttttattgc aattttatta acctttgata gtataaattc agatgattca
    25981 tcgactaaat cattatattt tttattataa attgaataac atccaaaaac tattaaccaa
    26041 ctcaaccctg gaagagaagc agtgcttgga cctttaaaaa tatacttagt ggctaataca
    26101 gctataaaaa aataagttat tacaagcaac tccaggataa gctttgattt tacaccagtt
    26161 tctgatttag acatataaaa tcactcctta aaaattatat tctctatact ctttaatttt
    26221 cgtttttatc aatattatca cttagttgca tattcccttt ttatcaaaat atatatacaa
    26281 aattaattta gataaagatt gtatatataa aatagctaag agaattacac ctatatccaa
    26341 atttgatata tccaaacctc ttgtataatt tgcttaaaag aaaacagcta ttagaaaaat
    26401 agaatataac attatttttg atgcaatatg atcaaccttt aataacataa gttttgatgc
    26461 ctcatcaact aaatcattat attttttatt atacatgaag tcacatgccc caaaatcgaa
    26521 taaaattata aaagtaattc aaatatttcc ccctttaaaa taaacttttt taataatgaa
    26581 tctataaaaa ataggatatc ataagtagct ttgaaacaag atttgtttta aatccaagct
    26641 ctgatttaga catataaact ccttaaaagt tatatattta taacacctta tgttataaat
    26701 atataacttt tccaaataaa ttgcaataga atatcgtaaa tattcaataa tatctttata
    26761 aatatagttt ataaaggatt ttataataaa cattgcaata cagtactaaa ttagtttatt
    26821 gcatatatta tttcaatcat atcatttact aactattatt ctaatgagca tataacattg
    26881 aaatttataa ttatacaacc taaatgcatc ccaatgtacc ctcaaaacct tcattttctg
    26941 taactgaagt ataattggta gattttatca ctgaaataaa aaacactagt caataaagac
    27001 tagtattttc aagtatttta tcattctttt ttttaattct tggagaagaa tcatcagaat
    27061 aatacctaca atttatctat atgcaaattc taaatttaaa ataaagtttc atatgaattt
    27121 actattctat gctatgatac aacagaaaaa actacaatct atgagcggta gcttcatata
    27181 atcctagttc ctacagtaat caaaaaggaa gtttattcta tatgtaacta atgataaatt
    27241 ctattatagg agttattagc tctatttgtg ttactataag tagtaattat atatataata
    27301 attttttaac acttttctat tggctaaata gaaagtagtt tagaacttaa atttaaataa
    27361 aatttaagtt caccaaaaga aatctatatt agaaaccact actctattcc accagattgt
    27421 aattttttgt tctttatatc ataatgctat accacattaa atttaaaaat aaattttaaa
    27481 tttatttgtt acaattcatt agttcaaaaa aatactttga gtcatgagtt ttgtaaagat
    27541 ttaaccatga atttaatgct tctgattcaa aaacctctaa aagtttcaat acttgaaacg
    27601 taaattccaa cggagcaatt cctggagctg taactaaatg ttcatcacta actacagatt
    27661 ccattttata atatttttct ccagaataat ttggtactac cattttaaga aactctaaat
    27721 cattacttgt atgatttcta aaatctaata gacctttttt agctagacct aatgtagctc
    27781 cacatatggc tgcaactata gaatcttgta ttaacgcttc tttagctttt tctaatatta
    27841 tctcatgatt catagataac caagtatttc cccctggtaa aattaataaa tccttacttt
    27901 ccatattaaa ctcatcaact gtaatttcag gtaaaacttt taacccaccc attgttataa
    27961 taggattttt atcaattcct acagtaatta cttttagtgg ttttaatcct tttttaaaat
    28021 atcttcctgt atttaattca gcagttaaat atgccacttc ccaatctgac atagtatcaa
    28081 atacatacat atatactttt ctatttttca tcttttttac tccttatgta attaatttat
    28141 atatctaatt gtaaaataac ttaactgaca tattcagtca gttaagttat taaatttgat
    28201 aataatcaat taattctagt aaatcttcaa tcattttctt cctaaggctt agtggttgaa
    28261 ttacttgtat tgtctttcta taaggtaaaa gtatatttgg tatgtacttg catattactt
    28321 cctcttcaag cataaatatt attttattag gtgttctttc tttcaagtag tgccctaaaa
    28381 accaatgttg gcttaaacta tctaaagaat ctatactccc ttctacaact aattgaatta
    28441 tactttgttc atcttttatt tcaggtataa tcccttttat aaaaaattca cttgccgaaa
    28501 attctgtcgg tctgttaaat gtatttgatg tttctatgat ttcactaatc ctatccactc
    28561 tgaaactacg aatctcattt ctcatattac aaaaggcaac tacataccaa ttattattcc
    28621 agtgaacaat tccatatgga tttataattc tatattttga ttttgcttta cgacctgcat
    28681 gatagtttat ttctacagat agctccttta atatactatt ctccaatttt ttaagtactg
    28741 attctataga tactttccca attggtttta ttacttcaag ggctattaag tggtttgtta
    28801 aaatatcttc ttgtttttga tttgaataca tttttaactt taatatcgat ttatctaatg
    28861 cttcgtctaa gaagtatcct tcttttttag caaatagagc tgcatgaagt agagattttt
    28921 gctcttctat atcaaaaaaa agaggtgctt tagtaaaatt atttaaaagg ctatacccac
    28981 cattatgacc tatatctgaa attataggta ctccactcgc agaaagtaca tcaatatatc
    29041 gatacactgt tcttatattt atttctaatt tttcagctat ctcttttgct gtaatttttt
    29101 tgtcagagct taacatccac agtatagaca tcatattatc aattttagcc atgtagtttc
    29161 acctctttct atttgttata taaagttatt atataacttt tgattgattt tttataaaaa
    29221 acattagtaa actgctaata atttatacta agatttattt caaatattta tagatagtct
    29281 ttttctatag ttaagttatc cattaaactc catagtagta tatctacttc aattaatata
    29341 ttttcaacta cacttctcgt tatatatcaa ttctcttttt taaagagttt acaactgata
    29401 tttagtttaa gatttatata tattattttt caaaaacctt tcaatctcaa attaaaactt
    29461 ctttgtttta atttgtttaa gatatagaaa aaatcttatc ctaatttatt taagctaaaa
    29521 ttcttatatt ttaaaatttt ctttaaaact aaaatattta ttatcatctc cagcaacaat
    29581 ataatctact aattaaatac ctaatattta ttcatatttt catagcttat tagttataac
    29641 tccatcttct tgctaaaatt aatgttaccc aacatataat tgtggaagta attactttag
    29701 atacatcagt taatacatct cttggatata ttagcactga gtttatagcc catctactta
    29761 caacttctat acttactggt tgccactctg tatctaaaca tattactaca aatctttctc
    29821 tatcactatc tacaaaaagc ttcttactaa tctataagca ttataagaat tacttttttt
    29881 ctagtttcat aaagtactac tttttttgtt aatttaaaaa atctatatct actcttttaa
    29941 aagatgtttt ctttttattc atttacctat ttcctatatc tttctgaatt gaaaataata
    30001 atgtacacta tatcaatgta tattgttata taaataaatg ttcagtttct ttatttctag
    30061 atattcaatt atactacatt cactagataa caaatatagt gttgaacttg tatatattta
    30121 cttcaccttt atatattctc cactaaattt tcctaataat ctttttaatc tatttgattt
    30181 aaaatactct ttaaaaaact ttacttcttt cttaccttca actcaactat acacacatat
    30241 ccactataat ctgttgattt tctgttatat ccatagtttt catcaagaac actgacaata
    30301 ttattcactt tttaagccac tccctttgaa tattattaca ataagtttaa atcttctttc
    30361 ttcaataaat atattatgag gaagactatc atctttaatg gtttaatagc gtataaggat
    30421 atgaatttaa aaaatctatt ttttatatca tatgggtatt gttttttata aaactggaaa
    30481 aacctaatag aatctatgat tttttaacat aacctccata aattagtacc aattataact
    30541 tcaaatttaa attttatcta aagaatatac aatcatattt ccagactcaa cataatattg
    30601 aatgttgcgc ttgtatttac aaattatcat tctaataaaa acaattatac atagcttaaa
    30661 atcatcttct aaacataatt actaaaattg aattaatgat atcaattcca ttaatctctt
    30721 ttaataaaaa attctctttt ctatatgaaa attatttcaa aactttaaag ttaaatacaa
    30781 aaatgactat aaatagattt ttatttacac aaatctatct atagccacta tagtttatcc
    30841 aataaaataa taaaataaat atgtacacaa taaatgttat ttacgtctta agtatttatt
    30901 tattagattt ttaattaaaa aaaacaagct agtgaacatt aatgctaaat ctagcattat
    30961 attgctttga tctcctgttt gtacattttg acctgcatta ctatctaaac tttcatctgt
    31021 atctatattt atatctggtt tcccatcccc atctatatct atatttacat ccgatttccc
    31081 atctccatct atatctatat ttatatccgg tttcccatct ccatctatat ctatatttat
    31141 atctggtttt ccatctccat ctatatctat atttatatct ggttttccat ctccatctat
    31201 atctatattt atatctggtt ttccatcccc atctgtatct atatttatat ctggttttcc
    31261 atctccatct gtatctatat ctatgtctgg ttttccatct ccatctatat ctggtttccc
    31321 atccccatct gtatctatat ctggtttgct atttcctccg ttatctgaat ccggtttgct
    31381 atttcctcca ttacctgaat ccggtttgct atttcctcca ttatctggat tcgggttatc
    31441 tggatctggc ttatttggat ctggattacc tgtatcataa cctcccgagt cagtatctac
    31501 atttttatca ggcttccaat aatcatctac tccatcgtta tctgtatcta ttgctggacg
    31561 attataatct gtatcagggt ttccatctcc attggtatct atatttttat caggtttcca
    31621 aataccatct cgatttccac cttctgtact aggcttccat tcacctgtat tatcagtatc
    31681 tatgttaata tctggcttcc aatcaccact tgtatctata ttgatatctg gccttccatc
    31741 cccatcagtg tctatattta tatccggaat accatctcca tctatatcta catttgtaat
    31801 atctaacttt aacttctttt catctttact atctttagtg taatttatat catctccaat
    31861 atattttaca gtaatagttt gttcagtatt aagaatatga tgattactat ctaaaggttt
    31921 ccattcaata gatgcagtac tataattatg actctcacta accttatcat gagtttttaa
    31981 ctctactgga tctttatatt cctttccatt aatatagaat tgtacataac cttttggaga
    32041 ggcacaatct ggtccttctt tcttatcagg tgaaacagta gcatttaatg taaacgtatc
    32101 tgttggttta aaaacatcag tttttacttt aggcgtttct acaactaatg aagtttctgt
    32161 atcagcagga gtaatttctg cataattata atgtgatcta tgcccccaat aagactcatt
    32221 aaaaggagct acactagcat actgtttcga tgtaatgatt aattgatatt ttccaacatc
    32281 aacttgtttt gtaaggtcat cacttcctaa acttgtatct ggttccatcg tttcctcatt
    32341 taaacgttga gaactaataa ttatatatgt tccatctgtg agttttccac ctttaggatt
    32401 agttactgga ataccatctt ttaatatcca atctaaaaat gctttttctt gctcttttcc
    32461 aaatggtaat ccatcgtatc ttttattaat cgaacctggt ggagtattta tctcatcaat
    32521 aataaattct tcatactgct ttaaaaatgg agtagttgga cctatatctt taatataaaa
    32581 agtatctgat tttagagtat ctccagtatc tttatctaca atagctaact taacagatac
    32641 ttcagatcca gtaaatttat cagagaacca taagtaaaga accccttgat catccgtata
    32701 gctaggcgct ccataaggat actcttttcc attaatttgc attgtaaaat catcgactag
    32761 tttagtatta actttatctt gccctaaata ctttagagat actttgctca taaaaacttt
    32821 tgttttcttt tctaagtcac cgtatgcaac tgtttcaccg ctacttttga atgttgttgc
    32881 ttttaatgaa ccacctgtta ctataacatc tcctgttata gcacttaggt caaaaatcca
    32941 gtctcctgct cctgtcatat aaggtaaaac cgttcctcca gttacaagta cacgatatcc
    33001 tctatttgct cctggattaa cacatccatc tccaaatgca ctaccatgtg cccctccata
    33061 tgaatagcaa agtccaccat taattgtaat atttccacta taaccaccac ctttgttacg
    33121 atctactact gatggactta atgtatgatt tcctgatata tagtacgctc cccctattcc
    33181 agctccatgg tatgcgcctt tagcagttac agtaccacca ttgattgtga tatctccacc
    33241 atcaccgata ctacctcccc ctattccagc tccaaatcca gtattatacc cttccatagc
    33301 tgtagcattt ataataccac cattaatgat aatatttcct ccattttcag tatctccacc
    33361 accaatacct gcagcataag aatttccgac agctgttaaa ctccctctgt ttttactatc
    33421 tagtagtgta aggcgatctc ctttttttag cttgtcccca ttttctagag ttacatctct
    33481 aggaattttc ccattttcag gaactataaa ttctccttca atgtctctat ttacaacaga
    33541 atcatcaata attagagtag tactttttcc ggctcttata cctggataaa gttgatcacc
    33601 tgactttagt atgtttttgc taccatcttg taatataata tgtgcatatg caccaccatc
    33661 tttatctggt gttaaagtaa atgggctatt agatgcagtt ttaatattta ctccattaaa
    33721 tattagttcc gcttttacat ctttaggtat aacaatccta tgtgttgttg gagtatttgg
    33781 attattattt tgaattgtca tttgttttcc actaagaata gttaaaacgc cactttcaaa
    33841 tttaaaatca acatttattt ctccaccgct aattttaaag tctcctacag aatcatactc
    33901 ttctgcatta gtatcttctt ctacaagtac ttcattagta tctccttctg catttccttc
    33961 attagtatct ccttctgcat ttcctgcatt agtatctcct tctgcatttc ctacattagt
    34021 atctccttct gcatttccta cattagtatc tccttctgca tttcctgcat tagtatctcc
    34081 ttctgcattt cctgcattag tatctccttc tgcatttcct gcattagtgt ctccttctgc
    34141 atttcctgca ttagtgtctc cttctgcatt tcctgcatta gtatctcctt ctacatttcc
    34201 tgcattagta tctccttcta catttcctgc attagtactt tcttctatag tatctgcgaa
    34261 agtatttgac acattaaatg atgctactga tccaagtccc attgagatac ttaaaatcat
    34321 tgataatgct ttctttttac atttttgttg cataaattcc ccctccccct aataattttt
    34381 aatatacact gtatacatcc taatatatac tctaataatt cttaatatac actgtataca
    34441 tcctaatata cactctaata ttttttaata tatacttaaa acttatattt gtcaatgaat
    34501 atactgtttt tttcaacctt atatattata agtatttatt atttgctaaa taactacaa
//
LOCUS       DAMJVM010000035        34030 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.035, whole genome shotgun sequence.
ACCESSION   DAMJVM010000035 DAMJVM010000000
VERSION     DAMJVM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34030
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.035"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            194..628
                     /locus_tag="PC523_001751"
     CDS             194..628
                     /locus_tag="PC523_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475276.1"
                     /translation="MYIENIEELKELIELFGVVEAKSENVVAFGEVESLDSCTEENAE
                     KALDILNKHKQENKKRYRDFFELKEWINGDEERKQIFADIAICERCGKIVANSEMLTS
                     FPILAKYDENRSVFEQLETVAICPDCLMFITRVYESLFDFEG"
     gene            726..887
                     /locus_tag="PC523_001752"
     CDS             726..887
                     /locus_tag="PC523_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475277.1"
                     /translation="MKKISEMSILEKADFIRNRQLELKKKYQNKAKGELFNMACRELE
                     EKEKCEGVF"
     gene            892..1236
                     /locus_tag="PC523_001753"
     CDS             892..1236
                     /locus_tag="PC523_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475278.1"
                     /translation="MLGYKLVRTEELLKLRKDLEIKEEELKRNLEELSEQHLSKASLY
                     MNLGMTITENEFYNSIIEKLFDKDFRKAISIFNKTKSIRIKNKKATEILLRIDREEKI
                     KEFPCRNKRRKC"
     gene            1239..1634
                     /locus_tag="PC523_001754"
     CDS             1239..1634
                     /locus_tag="PC523_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2475279.1"
                     /translation="MNNVCLVGRLTKDPEFKYIPKSGTPVATFTIAIDRDYVNKEGQK
                     ETDFIPVEVMGKSAEYCASYITKGRLVALKGEIRVDSYTDKKTEEKRIFTKVHTKMVT
                     SLESSKNNKENKEETLNLQAIYDDPDIPF"
     gene            1692..1838
                     /locus_tag="PC523_001755"
     CDS             1692..1838
                     /locus_tag="PC523_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475280.1"
                     /translation="MTRKGKVLKICGWCGKTFLSDNDGKIAYCSKKCKKKREKNLETE
                     GAKK"
     gene            1948..2217
                     /locus_tag="PC523_001756"
     CDS             1948..2217
                     /locus_tag="PC523_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475281.1"
                     /translation="MENIFWWSFMDLEEAEKVVTEESIKDLENAINEIENEAEEVKIL
                     IIKKNGDEKYINFKHDGYMKDVIKDRIVQCFEEIKIMHETILEEI"
     gene            2236..2496
                     /locus_tag="PC523_001757"
     CDS             2236..2496
                     /locus_tag="PC523_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475282.1"
                     /translation="MNFKMIANVPESWTVQKQFEKILEEILELKEAIALDDNKKILEE
                     GLDVFQAILTLFKIIGIHNISEGLKEHNKKLRRRKWKLEKID"
     gene            2744..3205
                     /locus_tag="PC523_001758"
     CDS             2744..3205
                     /locus_tag="PC523_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475283.1"
                     /translation="MKIKKEYIDKVENILKMLGDEYRKLRVLKKEIAILKHKENYREI
                     NYEELGFKVQKSVKSIDDMVVTIEDVIYNKETEIEIIERKLEFYNIYLKELSEIEQEI
                     IELVYFYSWDGKMPITKMARELNYDRSSLYNKKAIAINKIALMIYGDEALE"
     gene            3308..3775
                     /locus_tag="PC523_001759"
     CDS             3308..3775
                     /locus_tag="PC523_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475284.1"
                     /translation="MVELKKEYIDKTIKLLDEVRELKVRKEVLNDEIEFLKKNEKLSS
                     INFNELGFPVRSGNYGIDDMIINSQEKIYIKETEIEIIDSRIEMINIYTKRLSEEEQE
                     IISLRHFDSKINSYGEISELLMISKTVVQRKYTDALRKIALMKYGEEAKKDRE"
     gene            3945..4523
                     /locus_tag="PC523_001760"
     CDS             3945..4523
                     /locus_tag="PC523_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2475285.1"
                     /translation="MKRTQEVLDIQDYLRYKSYRNYVIFMLGITTGYRAGDLVKLKVR
                     DVREALRRQEFTIMEGKKANSKNIREKNRKPRTVEIRPKIAQILKKYIKGKQDYEYMF
                     TSRKRKYPYIGVEAISKALKEAGEYFGLYDITAHSMRKTYAYKIYIDSGKDIVAVKDL
                     LGHSSIEETKAYLGLDKELYHRYSESLDDFVR"
     gene            4720..5010
                     /locus_tag="PC523_001761"
     CDS             4720..5010
                     /locus_tag="PC523_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475286.1"
                     /translation="MKEIELDDKEIKLIISALDLKISRNLDKAYNSFPYSFDEKEEYK
                     RDVATMELLIDKFLSKLDEVECEEVDKEALVIDWNIEEFKEILKEAIEGVLI"
     gene            5010..5303
                     /locus_tag="PC523_001762"
     CDS             5010..5303
                     /locus_tag="PC523_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDF2475287.1"
                     /translation="MAREFSRSFYNSKAWKECRQSIIKKHLGLCAECGKLGEEVHHIK
                     YLTPANIHDADIALGEENLILLCKDCHSKKHKSKKDITRAGLKFNENGELISI"
     gene            5397..5792
                     /locus_tag="PC523_001763"
     CDS             5397..5792
                     /locus_tag="PC523_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475288.1"
                     /translation="MRRGGGLFKIIYEQLEKEKKIKQEVSRLKKNYKDLEKEKVKILD
                     GLVNEAAFLKISLEETREILTKEGLTEIFKQGKQEFERERLQVKIYLNFMKLYSSVMK
                     QLIDIIPSNTKQEEEDELIEFIKKGRLQK"
     gene            5789..7438
                     /locus_tag="PC523_001764"
     CDS             5789..7438
                     /locus_tag="PC523_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDF2475289.1"
                     /translation="MTYIEEYYQKILSGEIVACSRIKQVYKKLVQDLYNPKGNWVFDE
                     ELANRPIEFIETFCKQAQGKLGEPLKLELFQKAKHQAVFGFVDKDTRFRRYQEVLDIR
                     GRKNGKTTELAADELFMLIGDGEGSPEVYNIATKYEQAQKGFKECYKMVQQSKVLTKH
                     VKKRKSDIYFHANYGFIQALASNSNGLDGLNSHMVTIDELAAIKNRDIYDLMKQSMGA
                     RNQPLLNCITTNGFVREGIFDAQYEYACNVLDEKIKDDRFIAFIYELDDKDEWDKEEC
                     WIKANPGLGTIKKLDFLRDCVNKAKTDPSFKPTVMVKDFNMKENSATAWLRWDELNNE
                     VKFNIDEMEFRYGVGCFDLAETTDLASAKVLLKKRYDDNIYVLSMYWVPSERLIQKTD
                     EDKIPYDLWEKQGLLRVCEGNKINPYDILLWFREIREEYDIYIPWIGYDPWHVDSSLF
                     LAYENEFGKDSMIKVRQGVYTLSAPMKELRADLKANKVIYNNNPIDKWCLSNMEIKTD
                     INGNIQPIKGMDRRRRIDGGVTLIIGYVVLKEKMAEYENMI"
     gene            7454..8608
                     /locus_tag="PC523_001765"
     CDS             7454..8608
                     /locus_tag="PC523_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2475290.1"
                     /translation="MNIFKSKKKNKEAPGKVVMELVSDSGNGFYSWHGNLYRSDIIRS
                     IIRPKSKAVGKMTAKHIRSNETEFKTNPEIYIKFLLENPNPFMSGQILQEKMVTQLEL
                     NSNAFAVIIKDSYNIPIQIYPLNALNVEAIYEDEVLFLKFSLKNGKKVTYPYSNIIHL
                     RKDFNENDLFGTPPTKVLEPLMEVVNTTDQGVVKAIKNSNTIKWLLKFKTALRPDDIR
                     KEVKEFEKNYLQIDSDAGGAAATDSKYDAEQVKAESYVPNAAQMDKAVQRLYSFFNTN
                     EKIIQSKYTEDEWRAYYESEIEPVGLQLSNQYTEKLFTKKERSFGNKIIFEASNLQYA
                     SMSTKLNLVQMVDRGSLTPNEWRKIMNLSPIENGDKPIRRLDTATVKGGE"
     gene            8614..9351
                     /locus_tag="PC523_001766"
     CDS             8614..9351
                     /locus_tag="PC523_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDF2475291.1"
                     /translation="MANNNLNEFLKIKNSTETSSDLYFYGDIVCDEWDAWAEEDQYPL
                     SIKNFLATEQGKDLNIYINSGGGSVFAGMAIYNMLKRHEGFKTVYVDGVAASIASVIA
                     LAGDKVVIPENAYFMIHKPWIGLWGAYNSDKLIKASQDLDRIEEGILNVYQDNLKEGI
                     DIEEIKEKLNEETWFTGKEASNYFNFEVDEKKKVAACVSDYFTKYNKIPYALKNKTDK
                     ISIGKENNNKKRVQMKLNLLKLGGLND"
     gene            9344..10612
                     /locus_tag="PC523_001767"
     CDS             9344..10612
                     /locus_tag="PC523_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDF2475292.1"
                     /translation="MTREEYFEKRQEMIDEAQKLLDDEIGEEGTGEGKTEEAEKIANK
                     IKALDEEYERNVKARANLRALQDDFKVNPTIFNLTNNKEKIEGIEDAIIEDEQEKYEN
                     AWIKKMQNRTLDLNENEVLSKVNMKYRNEIRTTNSDAILIPETVSKGIWTEIGNLYPL
                     FGDTSPTFVKGDFVIIKEENSGDDASWYDEATPIKEDGYTLGEITLRGCELAKDITTS
                     FKIENMANEDFVPYISKLLAEKMGAALAKAIYSGKGKPGSSESFVAEPRGIKTALMAE
                     VEKPQIIEYTDSIGYSDLTKLMAVLGKWGNGACIYANNTTIWTQLAEIKDSMGRPLFI
                     PDMVNTEGVGRILGKVVKADDSINDGEIIAGNVSRGYAININKDVALDSQRDKKKRTI
                     DFISYCIVDGDVISNKAFGMIVKKTSAIAK"
     gene            10622..10909
                     /locus_tag="PC523_001768"
     CDS             10622..10909
                     /locus_tag="PC523_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDF2475293.1"
                     /translation="MIVTLEEIKEYLRLEADYEEDDNLLLSFLKAAEEDLENRTGKVF
                     NENNNSELVSLYIKMYVAEQYEKRGATEGNSEKVRFVLESIISQISICSRY"
     gene            10913..11251
                     /locus_tag="PC523_001769"
     CDS             10913..11251
                     /locus_tag="PC523_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDF2475294.1"
                     /translation="MDIGKLTQRIDIQIYGEIENDIGEVTKGWATYKKLWANKSLLRN
                     SNNYVLDKENIEYSYRFKIRYRTDITEAMRIVCNDVVYDIKHVNNIKELNKYETNIDC
                     ILYKEGVYNE"
     gene            11244..11657
                     /locus_tag="PC523_001770"
     CDS             11244..11657
                     /locus_tag="PC523_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDF2475295.1"
                     /translation="MSNTDFNMNGLDDYTNKLFKRIVKEYPKKAEKLMNISLGKCKGE
                     AIARTPKSDKKPKKYKRAKHMKDNWKTKTQSKNGNFVGVLKNNSPHAYLIENGWVTKN
                     GGYVEGKHMLQQTMEHQRAKIDKRIEKMVDETFNL"
     gene            11669..12085
                     /locus_tag="PC523_001771"
     CDS             11669..12085
                     /locus_tag="PC523_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475296.1"
                     /translation="MLKIVSVKKAIVEKLKSLNIKIVANEIRSGFEKPAFFIQIIPIE
                     MASDPSFSNSTLLVNIHYFSKEKTELENLKMIDKLNILFQDCILEIEGGKLTIEEKSV
                     EIYENVLQYKFNLQVVEIIEEDESKYELMKELEMNI"
     gene            12102..13169
                     /locus_tag="PC523_001772"
     CDS             12102..13169
                     /locus_tag="PC523_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDF2475297.1"
                     /translation="MGLPSAIIEFQRRSRTVKFRSRRGIVALILKDSTAIKKSYSIDF
                     LTDINETEFTKENYDYIRLAFLGKPSKVIIEVINDSVDSERSLDDALKALRENKFNYL
                     AIPFISEEVDKTKIVNWIKTARREKEIYKAVLPSVTNANEKAIINFSTTGIKVGEKVY
                     TTAEYTARVAGILAGISLSESCTYFVLDEVTEIEPTENPDEAVDEGKLILINNNGIRI
                     ARGVNSLITLSKEDTEDLKKIKIVEAIDMIQDDILQTWNENYVGKVTNKYDNKILFLS
                     AVNNYFKELQRDEVLDNSQEAYAQIDIEAHKKYLKEKGIDYSEMTEQQIKEANTGSYV
                     FIEGNITVTDAMEDLKFKIYM"
     gene            13183..13632
                     /locus_tag="PC523_001773"
     CDS             13183..13632
                     /locus_tag="PC523_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2475298.1"
                     /translation="MGKENIVGSSQISGTWGKLWWDGTLIAEVLSFEAKVTANREEIQ
                     FGMSKDSKITSLSGEGTVKLGKVYSRGKKKLLEAWKNGEDPRSTLTSKVKDPGTPGKQ
                     AETVTINNVWFNELALSQFEKGGKIEEELSFGFTPNDSDVMDEIDEI"
     gene            13679..14062
                     /locus_tag="PC523_001774"
     CDS             13679..14062
                     /locus_tag="PC523_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2475299.1"
                     /translation="MDNKKEMVTIEDILRRKEYFAKKSEETKQLYIPSLDGNIEISKP
                     DRMLCLDAIEMEDAVEGDKYFVYEIVKSPNLKSEKLHAEFGCKDNPLDIVDVLFEAGE
                     ITDIVKIATKFAGFGVVEEIEDLKN"
     gene            14086..14253
                     /locus_tag="PC523_001775"
     CDS             14086..14253
                     /locus_tag="PC523_001775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475300.1"
                     /translation="MISHYLEKGVDLDKLTNLNMIEKKFHIACMLYEEEEKIKLISEL
                     IGAMFGGVKNG"
     gene            14246..16000
                     /locus_tag="PC523_001776"
     CDS             14246..16000
                     /locus_tag="PC523_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475301.1"
                     /translation="MARRHIGAVISLKDNMSATMRGIRREQKQFQNEVRRTRNEMRSA
                     SRERMRIRMDATPAHRTIQDLRRKFAPLRTKLVKAVVIKDLATEKIERIKSNVKSFGK
                     FIARPVVKLKDETKGMIDKIKNRLTSLSTIVPVGAAVGAAGMAVKSGMELEQQQISMR
                     HFMGVGNKGKSSKELDGMSASYLKDLRNNANATPFETGEVISAGTRSLQIAGGNTKDA
                     MQMVKLAEDMAALNPGKTVGDAMEALADMNIGEMARLTEFGVKASSTDDPKEVQKKLE
                     TMYAGGANKLAESGSGLLSTIMGKLKSNIADIGLGMLEPLKPVMTGLIGFIDQASPKI
                     LEVGTKITSGIGMAIGWIQQQMPTLAPIFQTAFGAVSSIVSTVAPIIGQVIGALSPVF
                     MGLLSVASSALSGIASAVKTVAPVVSSLISKLAPIFLNVGSTLKSMGKIFKNVFDSVM
                     KIVKKASDFIKPLVNGIIGATKGISDGVSWVAGKLAGNATGTKYWSGGLSVVGEHGPE
                     LVSMPRGSKVFTNAESKSMINKSIPNFRQVQGENTNYNITIPKIAEQIVIREDADIER
                     ITSSLIKKIQMAKMGGVV"
     gene            16000..16638
                     /locus_tag="PC523_001777"
     CDS             16000..16638
                     /locus_tag="PC523_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDF2475302.1"
                     /translation="MEMWLRQSNDSFRFPILPTSFEISGNINTSTTNVLKLGEVIVCG
                     GMGLRTTEINSFFPGEQYHFCNYKDFPQPYDCVNKLKKWMEQGLILRYIITETDVNME
                     VIIESFKHGKQDGTNDVYFTLSLKEYKRIQIPKVNTNNDEKLSSVKDVPLTKGFEVKK
                     QRTHKVSKGDSLWSLAKKYYGNGDLWKKIYDANKKLIKNPDIIQDGWVLVIP"
     gene            16878..18398
                     /locus_tag="PC523_001778"
     CDS             16878..18398
                     /locus_tag="PC523_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2475303.1"
                     /translation="MIINRSKDSSNNSISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFNDNKLAIGNIPKTNVKYTKMFIGVTGYDTIMSAYTEASKTTKKKYMIESNLDKFN
                     VIEKGIITLNVMFEEGSNLINTSFSESMENVKNKVLVVDQYGNKISEKIDDKIFKDVG
                     VIMQKVIQQQENSTVDIESEFKGIEQTCSLKGYGDVTCITGRGVKVKDSYTGLTGLFY
                     IDTDKHNWDSNGNYEIDLDLNFQNIMDEKTAGQDEQKEESSDLNGEGTLNGREVKAEF
                     TAYYPSNNPMEGGYYQAMDGKRLVPSNNTCAAPSKLKFKTKIQAKCPGTKIDGKTYTV
                     TDRGGAIGLKNGVYRIDILMSSEKECNDFGRRKGTIIIGDGTGYTNATGKAKELISIA
                     KSKLGCKYVWGATGPNTFDCSGFTQWCYKKIGINIPRVSRDQGKAGKAVSKGSLQLGD
                     LVFFSSKGANGAIDHVGMFIGSGEFIHSPHTGDVVKISKLSGSYYAKNYVTARRYL"
     gene            18414..18740
                     /locus_tag="PC523_001779"
     CDS             18414..18740
                     /locus_tag="PC523_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HDF2475304.1"
                     /translation="MANPINEFIGIIREEGKHYNEPSFFIGEIKSKLPDLKIEVNNVI
                     LEKEDILIDSWILDRQIETFNTETSQEHRHEVKNPFIDTFEEEDTVIMFKIGDKFAVV
                     SKLVSL"
     gene            18740..19168
                     /locus_tag="PC523_001780"
     CDS             18740..19168
                     /locus_tag="PC523_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2475305.1"
                     /translation="MSTIFPFIGVPEDYEIPKTEELELFKEVAWDFEKDEPVVENGDF
                     KVIEGNEAIKVWVYKCIKTNKYEHEIYSWGYGTELSELIGQKYSKGLTESEASRYIKE
                     ALLVNPYILDINVKNTKFTDDLLSVDIEISTIYGEVEVNV"
     gene            19161..20210
                     /locus_tag="PC523_001781"
     CDS             19161..20210
                     /locus_tag="PC523_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2475306.1"
                     /translation="MYSSQTYDVIKNRTLSNIDLDIYKSEGSFLSDMVSPISTELAKF
                     YIELSYLHKKAFIEDNFDDFLDKRVNEFGVYRKLGTEATGEVIFEGKAGTIVQNGTII
                     SYNELLFVVIKDIVISSEIEQNTSPVQALEIGIRYNIPANTEFKLQDEINGITKIYNN
                     LDFKGGTEIETDEELKERFYKIQKNQATSGNKAHYEAWALEVEGVYNAKIYPRWDGPG
                     TVKVLIFGENNQAVDTEVIERCKEHIEIEMPIGPALTVLTPTILDINISASIKLEAGY
                     TLDFVKESFLESINSYLINVNKEIIYTKVSAILASTEGLHDFSNLLLNNKAGNIVFEE
                     DKVPSVTTLNFSEVV"
     gene            20211..20828
                     /locus_tag="PC523_001782"
     CDS             20211..20828
                     /locus_tag="PC523_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDF2475307.1"
                     /translation="MKLIDKLPSFYNNDVTRKIQEAYDIELETLRETYDDTFDQFFVD
                     TATWGLDYWENILSIKNRFDLSIEDRRSNIKAKMRGKGTTTIEVIKAISEAYTKTNVD
                     VEVFSNLFSFTLSFITNDCSYNTILELDKKIEEIKPCHLEHKFEMILFNQNNMFTGST
                     MNTGETVTIYPYSPTDITSFGELTITTGNDRSMEKVTLYPRQEVV"
     gene            20830..21612
                     /locus_tag="PC523_001783"
     CDS             20830..21612
                     /locus_tag="PC523_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2475308.1"
                     /translation="MAEQKYYTLLTTIGKASIANATALGNKVDLVKLQLGDGAGAEYN
                     PTEEQTSLKNVVWEGSINNVKIDEENPNWIVIETVIPGSVGGFMIREVGIFDSKEQLI
                     AVSKYPETYKPTADSGSVKDLVIKIILVVSNTSSVNLKVDPTVILATLKDIQDLDTKI
                     DTTKTELTSNIETAKTELNTRIGDTTQLTTTDKTNIVSALNEVKSSVDSIETTAEKTS
                     IKDADNLFESGNVEGALKEVMQEVKGNRSSIISTVNNNLIPM"
     gene            21629..23119
                     /locus_tag="PC523_001784"
     CDS             21629..23119
                     /locus_tag="PC523_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475309.1"
                     /translation="MRHYKRTTEMKKRRGTYSETDLYAYQQAFSYTIEPDWNYVGTFE
                     RSKIKNISINQNTNIFLTTNNIYKIRYSDNSKTTVTDLEMKQILDITFIKDPVINLTL
                     SDISKGCTIDYSITDNEPTQKFIVTEKLNNTVIATKLNSIGGNYSIVLTDEQILPLQV
                     NSQNNITIEISTEQGGLVVSKTATFTRINNKPTVLVSSYNSNSAKFTASDLDNNLSKI
                     EWYLDDVLKETITTDLTAEKTINYELTDNAIHTLKIVATDAENATVEKVLSISKEIMP
                     LQTDASLSDISTKLIEIGEGFKNGKTSIINTLALKNIEASLNNTLVELSEKIKTSFDS
                     SDASVQDLMNQLTQANNTISQLNSKYKYASGTIDVVKNSSLMANLYGESFGRQPGTWL
                     KIDNLGFIPNIFVAECQYVTSNNYFFKHIVIATCNINWLWEKKDFSARIVFTKEKKSN
                     QDFSGSGIIYSNNERDVYINNKGINVPASSPNASDYLHSWHAIKFI"
     gene            23136..23429
                     /locus_tag="PC523_001785"
     CDS             23136..23429
                     /locus_tag="PC523_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475310.1"
                     /translation="MNRANRIICDQTGKILLQTGEATGDILEHDEITELHFVDIPYGS
                     VDYTKSRIVGINIETKEPILEEMPVYLTEEEKRIQELENQLLIAENEKVGGIL"
     gene            23429..23611
                     /locus_tag="PC523_001786"
     CDS             23429..23611
                     /locus_tag="PC523_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475311.1"
                     /translation="MNINNVVVRILAERILNKGLNPLKNREFELDDVTNTEYRKAVED
                     YIIEHSGVVEGTEPTK"
     gene            23646..23876
                     /locus_tag="PC523_001787"
     CDS             23646..23876
                     /locus_tag="PC523_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HDF2475312.1"
                     /translation="MNEELLEADLKRHETRINKHGEEIDELKIANIESKAELKALCEN
                     LNSLTSMLKWLIGTMITTLVGFFIFAIQKGIF"
     gene            23892..24149
                     /locus_tag="PC523_001788"
     CDS             23892..24149
                     /locus_tag="PC523_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDF2475313.1"
                     /translation="MDNLISFIPEQLLLLVVALNVLGFGFKKYKQLDNKYIPIILLVL
                     GIVFSIWMLGFNPSSILQGILCWGVAIGANQVYKQLKEENK"
     gene            24149..24946
                     /locus_tag="PC523_001789"
     CDS             24149..24946
                     /locus_tag="PC523_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2475314.1"
                     /translation="MKIAIVPGHTLSGKGTGAVGYIDEGKENRILTDLIVKWLKQGGA
                     TVYTGKVDKSNNYLAEQCQIANKQNVDLAVQIHFNANSTTLNPMGTETIYKTNNGKVY
                     AERVNDKLATVFKNRGAKSDVRGLYWLSHTKAPAILIEVCFVDSKADTDYYIRHKDIV
                     AKLIAEGILNKNINNEGVKQMYKHTIVYDGEVDKIPATVVGWGYNDGKILICDIKDYV
                     PGQTQNLYVVGGGACEKIGSITKENYTMIKGNDRFDTLYKALDFIKK"
     gene            complement(24973..25182)
                     /locus_tag="PC523_001790"
     CDS             complement(24973..25182)
                     /locus_tag="PC523_001790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475315.1"
                     /translation="MKDVFEELISIKDACELYKKGESTLRLNIRNGKFVEGVDCKKFG
                     TTWVFKKTALEREYNKKDKKIESGN"
     gene            25331..25630
                     /locus_tag="PC523_001791"
     CDS             25331..25630
                     /locus_tag="PC523_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475316.1"
                     /translation="MKKITKKEIKKYVREAVNNNFNWEINKCGFYIKNNEIEFFISYK
                     GQGIDENVYNDTYEEIIYIEDIIEEYRRKEYNLEDVDSIVYDNVNDMINCYNEEK"
     gene            25719..26354
                     /locus_tag="PC523_001792"
     CDS             25719..26354
                     /locus_tag="PC523_001792"
                     /inference="COORDINATES: protein motif:HMM:NF013043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475317.1"
                     /translation="MKIGDKFENLTILDIEQKNSRKYCLCKCKNCGNEKWIRADSLKR
                     IKGCGCLQKETQFKQNDLTNKKFGRLTALKNTNKKAKSGHYIWICKCSCGNEIKTTEN
                     NLTTGRTKSCGCLKKESNIKNAKIALKVHKEKNIIDDTNLSIIKKTEAYPNSKTKIRG
                     VSWNKEKRKYCAQIEFKKTHYNLGYYDNIREAEEAYKKAKEKFLKEINGKV"
     gene            26431..26901
                     /locus_tag="PC523_001793"
     CDS             26431..26901
                     /locus_tag="PC523_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475318.1"
                     /translation="MNKQKARRFLRVIDMNIDKIEEEAIKAFKESCLIKETNNIKIYI
                     DIQGKVEAIAVQTWAKLLDDDKEINIFTLNQAPTHLDDMFGEIYHVNDYEEFEKWCEK
                     EDKSLNWDSYEKFNKENFEEIAERNIDDSSSAFLEKLREGIENCKQELQNIVEN"
     gene            complement(27144..27425)
                     /locus_tag="PC523_001794"
     CDS             complement(27144..27425)
                     /locus_tag="PC523_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475319.1"
                     /translation="MNVYLQIGKELSEKMTKSELNKFSQEVVNFLRYAGFDKDMKIKL
                     FQTIIEAVARKKVSLPSDSEFTEGILQSDEAEMTMNVGFLIEGLSYHNN"
     gene            complement(27569..27901)
                     /locus_tag="PC523_001795"
     CDS             complement(27569..27901)
                     /locus_tag="PC523_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016040102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475320.1"
                     /translation="MPSFNRDLLVDKEKPILKNGTIAQNDSTTTDNKTLNIYNLVNKK
                     SKKVTMSATLDEELVNKLKSFSIDMNSDVSKLLSDILTQILSDVTIKEENLNIYNERN
                     RRNKTKKK"
     gene            complement(27902..28711)
                     /locus_tag="PC523_001796"
     CDS             complement(27902..28711)
                     /locus_tag="PC523_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDF2475321.1"
                     /translation="MANTKLLTYFNIKGGIYKTTTSIMTAYELAKDKDKKILLWDLDV
                     QANLTQYVYEINHNDNTTLDILKGISANDAIVKSPNENYINVDLIPSDIQMARFEQEL
                     SPLPAREKFLARWYMQNFNTLSEYDYIICDLSPRYDLTAKNVLFLADSIIIPIQDKNI
                     SSLRGAELFKQLWDVDRTYFDKEDNIKSTILVGFEKKKTQISDTFDSYLEGFNDMRDI
                     MLDTYIRKNEFIEKALLKKLSLTDYTKITKEHFSRQEFTNMLEELKRKGVL"
     gene            complement(29273..29995)
                     /locus_tag="PC523_001797"
     CDS             complement(29273..29995)
                     /locus_tag="PC523_001797"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDF2475322.1"
                     /translation="MFNSVKRFLKTLKEPVLTEFDIAKQYDFSKELSSGIVSSFKSQK
                     EIEYEKFIKFTNSKNKVLEHYIVFDVETTGLDAFKEDIIEIAAIKFERDVPSEIFSTF
                     VKPSKSIRKKITDITGITDEDVVNAPSIEEVLPHFLNFISDYTLIAHNAIFDMEFILD
                     KLYKNGYKKITNKVIDTLTLSRKYIRTYEGKKLKSYSLPSLHEELGLNYSSHRALEDC
                     KSCASVYKACKSEMKFKDELVY"
     gene            30440..32593
                     /locus_tag="PC523_001798"
     CDS             30440..32593
                     /locus_tag="PC523_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF2475323.1"
                     /translation="MKNNINKLFLEECNAERAEHEKENYSNFNRHETYEIKKYINEKK
                     LGIKTAIANALDGGKTLFIAPTGAGKSYSFINTLKKLKTKALFILPNASNVEQAMHEY
                     NIAGAYDKIPAKQALENNNLAVMTWDKTEQLIDVDLREYIIVVDEIHQTYTDSYRGKA
                     IKNLNNIMSKCKGRIDITATPTKLEFEIYDYIIEYTQKQKTEYDVKLYNDFDNKNFTE
                     IINIINKSNNSAMLMNDISTLEFIRDSVNKNAGVVYADGKEENELYSRIVKNSDMKGY
                     ETLLNTTTILAGVNINNKDITDIIIVNIKDIGAIKQYVARFRNLKKVNVHIFNKYKEE
                     CNVYKIEWLVDKNIEKATILKDAYNKVSKHTLMFETVGINATPIRIDSNVYYCMKDNC
                     YKVDKLYIKSQIYSNYYNTRTIQSFKVLLEEYFDNVNITDAKEIETNEKELKEYKKVV
                     KEIKEATRNILKEHKEILVGYRQIKSNSKSFSLMQYHNDMKLSSKGCLEAYRAYDIHN
                     LVKKSKSNSMLELYSNYVLDNKFSLDLAWKLANTANRKRGAIFNKLNTLIYRELKEEY
                     PEFLNDELIQVTVFNYIDKLFGIGTSYTELHLQELSDDLRLILGENWNLTTKKLSIIL
                     NEVFVIEKKKYRGGNSLENIFFYKNINPKLFPLEAERMQVNNIKRYISIDDIKKDLKL
                     DEKDKSLEHAIKHTKDRMLNSLDEDEKLLLLKGFM"
     gene            32614..32952
                     /locus_tag="PC523_001799"
     CDS             32614..32952
                     /locus_tag="PC523_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475324.1"
                     /translation="MKLRIGIIDKYVAEASYELSELLIDAFNKYDEFVEAIRGGEIRI
                     VPFNLGFQALLEEDSTYDEYEFYYDDEDDKCFFHIKYRNVKDVELSKELLLELIKVNL
                     PAGAKLIVNN"
     gene            complement(33426..33779)
                     /locus_tag="PC523_001800"
     CDS             complement(33426..33779)
                     /locus_tag="PC523_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2475325.1"
                     /translation="MNIRIRKLRSSEGLKQIEFGEKLGKTKHEIYNLESGRTKVKESD
                     LKLIISLFKVNENWLRTGTGEMYNTEDRNSVKAEAFCAIDENEKLAKAVLEFSKLTDE
                     QLESILKILEVFSKE"
BASE COUNT        13731 a         3832 c         6288 g        10179 t
ORIGIN      
        1 aaaaaaaaca aaaagatatt gattgtataa taaaggctaa taacattgga aatatcgcta
       61 atgaacgtat tttagacata gataaattga acgaggcagt cgaagaaatg aaaaaaatga
      121 tttgttaaaa aagtgtactt gacggctaca ctaaaaaatc taatttaaaa aaactaaagg
      181 aaaaggtgag attatgtata ttgaaaatat agaagaatta aaagagttaa ttgaactttt
      241 tggagttgta gaagcgaaga gtgaaaacgt agttgcgttt ggagaggttg aaagtcttga
      301 ctcttgtaca gaagaaaatg cagagaaagc attagatata ttaaataagc ataaacaaga
      361 gaataaaaaa agatatagag atttctttga attgaaagaa tggattaacg gagatgaaga
      421 acgaaagcaa atatttgcgg atattgctat ttgtgaaaga tgtggtaaaa tagttgctaa
      481 ttctgaaatg ttaacctcgt ttcctatctt agcaaaatat gatgagaatc gaagtgtttt
      541 tgaacagttg gaaactgttg ctatatgccc agactgcttg atgtttataa ctagagtata
      601 tgagtcgtta tttgatttcg aaggttagaa acaaaaagta acaatgttag tcgtcaagag
      661 tgtttttaat agcattaaat tagttaaaat aagtatctta acatatgtaa caaggagtaa
      721 aacaaatgaa gaagatttca gaaatgagta tattggaaaa ggctgatttt attagaaata
      781 gacaactaga attgaaaaag aaatatcaaa acaaggctaa aggagaattg tttaatatgg
      841 cttgtagaga gttagaagaa aaagaaaagt gcgaaggggt gttttgatga tatgttgggt
      901 tataagcttg taagaactga agaattgtta aagcttagaa aagacttaga gataaaagaa
      961 gaagaattaa aaagaaacct tgaagaacta tctgaacaac atttatctaa agcaagtttg
     1021 tatatgaatc taggaatgac tataacagaa aatgaatttt ataattcaat aatcgagaaa
     1081 ttgtttgata aagattttag aaaagctatc agcattttta acaaaactaa aagtattcgg
     1141 ataaagaata aaaaagcaac tgaaatatta cttagaattg atagagaaga aaaaataaag
     1201 gaatttcctt gtagaaataa aagaaggaag tgttagaaat gaataatgtt tgtttagttg
     1261 gaagattaac aaaagacccc gaatttaaat atattccaaa atcgggtacg ccagtagcaa
     1321 cttttacaat tgctatagat agagactatg taaataaaga aggacaaaaa gagactgatt
     1381 ttataccagt tgaagtaatg gggaaatctg cggaatactg tgcgagttac ataacaaaag
     1441 gtcgattagt cgctttgaaa ggtgaaataa gagttgattc atatacagat aagaaaactg
     1501 aagaaaaaag gatatttacg aaagttcata caaagatggt aacgtcttta gaaagtagta
     1561 aaaataataa agaaaataag gaagaaactc tgaatttaca agctatatac gacgaccccg
     1621 atataccgtt ttagcagctg aaagaaacag agaaaaagct ccgttttgga attaaaaaag
     1681 gatgtgggaa aatgactaga aaaggaaaag tattaaaaat ttgtggttgg tgcggaaaaa
     1741 catttctttc tgataatgat ggtaaaattg catattgttc taagaaatgt aagaaaaaaa
     1801 gagaaaaaaa tttagaaaca gaaggggcaa aaaaatgaaa gagtataaaa ttgaaattga
     1861 agaaacaata tttttaaaac ataaagttat tgttgaagtt ctagagtgaa aaaaagctgt
     1921 aagaaaataa aataaggaga aaaaagaatg gaaaatattt tttggtggag ttttatggat
     1981 ttagaagaag cggaaaaagt tgttacagaa gaaagtataa aagacttaga aaatgctata
     2041 aatgaaattg agaatgaagc ggaagaagtt aaaattttaa ttattaagaa aaatggcgat
     2101 gaaaaatata ttaatttcaa acacgatgga tatatgaaag acgttataaa agatagaata
     2161 gttcaatgtt ttgaagaaat aaaaataatg catgaaacaa ttttagaaga aatataaaaa
     2221 gggagaaaat aaaagatgaa ttttaaaatg attgctaatg tgccggagag ctggacagtt
     2281 caaaaacaat ttgaaaagat attggaagaa attttagaac ttaaagaagc tattgcgtta
     2341 gatgataata agaaaatatt agaagaaggt ttagatgttt ttcaagcaat tctcacttta
     2401 tttaaaataa ttggtataca taacataagc gaagggttga aagagcataa caaaaaactt
     2461 agaagaagga aatggaagct agaaaaaata gattaaagtt aaaggtatga ataagtggca
     2521 tttactttaa ttattaaata gataagatag aaataattta tattaataga ttttatttga
     2581 acttaaaaaa atgaagtctt gtaacaaaaa aacaaaataa aaatccagaa tattttgaat
     2641 aatatgcagt agttggaaat gctacaaaat acctaactag tcccaaaagc taaatttaag
     2701 gattagttag gtatttataa caaaaagtgt aagaggtgca aaaatgaaaa taaaaaaaga
     2761 gtatattgac aaagttgaaa atatattaaa aatgttagga gatgaatata gaaagttaag
     2821 agtattaaaa aaagaaatag caattcttaa acacaaagaa aactatagag aaattaatta
     2881 tgaagaattg gggtttaaag tacaaaaaag tgtaaaaagt atagatgaca tggttgtaac
     2941 aatagaggat gttatctaca acaaagaaac tgaaattgaa attatagagc gaaagctaga
     3001 gttttataat atttatttaa aagagctatc agagatagaa caagaaatta tagaactagt
     3061 atacttttat agctgggatg ggaaaatgcc tattacaaaa atggcaaggg aattaaatta
     3121 tgatagaagt agtctttata acaaaaaagc tattgctatt aataaaatag ctttaatgat
     3181 atatggtgat gaagctttgg agtaaacatt atttttacag aaaaaagact atttttatac
     3241 ttcgtttcta aagaaagtat gttagatagg cttttttatt gctaaaaaaa ggaagagggt
     3301 gagttgattg gttgaattga aaaaagagta cattgataaa actataaaac ttttagatga
     3361 agttagagaa cttaaagtaa gaaaagaagt tttaaatgat gaaatagaat ttcttaaaaa
     3421 gaatgaaaag cttagtagta ttaattttaa tgaacttggt tttccggtgc gctccggaaa
     3481 ttatggcata gatgatatga ttataaattc acaggagaaa atttatataa aagagactga
     3541 aattgaaatt atagatagta gaatagaaat gattaatatt tacacgaaga gattgagtga
     3601 agaggagcag gagattataa gtcttagaca ttttgactca aaaataaatt cctacgggga
     3661 aatttctgaa ttattaatga tttctaaaac tgtcgttcaa aggaaatata cggacgcttt
     3721 gaggaaaatc gcattgatga agtatggaga agaagcgaaa aaagataggg aataaaaagg
     3781 actaaaaaag gtcaacacaa aacttttgtc gcaggttata attgtattgt ggaaaattaa
     3841 aaatgtttct acttcaaaag cttatgagga aaaactcatg agcttttttt attctctttt
     3901 gaaagggggt attttgatta ggagtaaaaa gagcatcaaa gcctattaaa cgtacacaag
     3961 aagtgttaga tattcaagat tatctaagat ataaaagtta tagaaactat gtgattttta
     4021 tgctaggaat aacaacagga tatagagctg gagatttagt taagctaaaa gttagagatg
     4081 taagagaggc tttaagaagg caagaattta ccatcatgga aggtaagaaa gctaatagta
     4141 aaaatattag agaaaaaaat aggaaacctc gaacggttga aataagacct aaaattgcac
     4201 aaatattaaa gaaatatata aaaggtaagc aggactatga gtatatgttt acatcaagaa
     4261 aacgaaagta tccttacatt ggtgttgaag caattagtaa agccttaaaa gaagcaggag
     4321 aatattttgg tttatatgat ataacagcac atagtatgag gaaaacttat gcttataaaa
     4381 tttatattga tagtggaaaa gatattgttg cagttaaaga cttgttgggt cactcttcga
     4441 tagaagaaac aaaagcgtat ttaggtttag ataaagaatt gtatcatcgt tacagtgagt
     4501 cattagatga ctttgtaagg tgatattttt ttatttgtct gtttgaatgt ctaaaaattt
     4561 ggtgtagtga tattcaaggt atcaaaatca ctatataaga agtaggaaaa aaataaattg
     4621 aatgtctgat tctctaagaa aataagacgt tcaaatgaag aaatacgaat tatatttaga
     4681 tggtttttaa taatgttcgt ataagaaaag aggtatttta tgaaagaaat agagttggat
     4741 gataaagaga ttaaactgat tatttcagca ttagatttaa aaatttctcg caacttagac
     4801 aaagcttata atagcttccc atattctttt gatgaaaaag aagaatataa gagggatgtg
     4861 gcaactatgg agcttttgat tgataagttt ttaagtaagc ttgatgaggt agagtgtgag
     4921 gaagtagaca aagaagcttt agtgatagat tggaatatag aagaatttaa agagatactt
     4981 aaggaagcta ttgagggagt gcttatataa tggctagaga gtttagtcga agtttttata
     5041 atagcaaagc ttggaaggag tgcaggcaat caattattaa gaagcattta ggcttgtgtg
     5101 ctgagtgtgg gaagctagga gaagaagtgc atcatataaa atatttgact cctgctaata
     5161 tacatgatgc tgatatagct ttaggtgagg aaaatttaat attgctatgt aaggactgtc
     5221 atagtaaaaa acataagagt aagaaagata ttactagagc aggattaaaa tttaatgaaa
     5281 atggggaatt gatttcgatt tagaaaaaaa tagcatatcc cccccctaaa aacgaccctg
     5341 gggggctgat ttcaaatacc gatgtcccca cctcaatttt cctccgcacg aaaaaaatca
     5401 gaaggggagg ggggttattt aaaataattt acgaacaatt agagaaagaa aaaaagataa
     5461 aacaagaagt aagcagatta aagaaaaact ataaagattt agaaaaagaa aaagttaaaa
     5521 ttctagatgg gttggtaaat gaagcggctt tcttgaaaat atctttagaa gagacaagag
     5581 aaattttgac aaaagaaggt ttgactgaaa ttttcaaaca aggtaaacag gaatttgaaa
     5641 gagaaagact tcaagttaaa atatatttaa attttatgaa gctttattct agtgtcatga
     5701 agcaactaat tgatattatt ccaagtaata caaagcagga agaagaggat gagcttattg
     5761 agtttataaa aaaaggtaga cttcaaaaat gacatacatt gaagaatatt atcaaaaaat
     5821 attgagtgga gaaatagttg cttgtagtag aattaagcaa gtatataaaa agcttgttca
     5881 agacttgtat aatccgaagg gcaattgggt ttttgatgaa gaacttgcta atagacctat
     5941 tgagtttata gagacgtttt gtaagcaggc acaaggaaaa ttaggagagc cacttaaact
     6001 tgaactattt caaaaagcca aacatcaagc tgtatttggc tttgtagata aagacacgag
     6061 gtttagacga tatcaggaag tacttgacat tcgaggacgt aaaaatggga agactacaga
     6121 attggcagcg gatgaattat ttatgttaat tggagatgga gaaggttctc cagaggttta
     6181 taatattgct accaaatatg agcaagcaca aaaaggtttt aaagaatgtt acaagatggt
     6241 acagcaatct aaagttttga ctaagcacgt taaaaaaaga aaatcagata tttattttca
     6301 tgccaactac ggttttatac aagcacttgc tagtaattca aatggacttg atggattgaa
     6361 ctcgcacatg gtaacaatag atgaattagc agctataaaa aatagggata tttacgactt
     6421 gatgaaacaa tccatggggg caagaaatca acctctttta aattgcatta ccacaaatgg
     6481 ttttgtgaga gagggtattt ttgacgcaca atatgagtat gcttgtaatg ttctagatga
     6541 aaaaataaaa gatgacaggt ttatagcttt tatttatgag ctagatgaca aggacgaatg
     6601 ggataaagaa gaatgttgga taaaagctaa cccaggctta ggtactataa aaaaacttga
     6661 ttttttgaga gactgtgtta ataaagctaa gacagacccg agcttcaaac ctactgtaat
     6721 ggtaaaagat ttcaatatga aagaaaattc tgcgactgct tggttaagat gggatgagtt
     6781 aaacaatgaa gttaaattca atatagatga aatggaattt agatatggcg taggatgttt
     6841 tgacttagct gaaactacag accttgcatc tgctaaagtt ttattgaaga aaagatatga
     6901 tgataatatt tatgtgcttt ctatgtactg ggttccatct gaaagactaa tacaaaaaac
     6961 tgatgaagat aaaatcccat acgatttgtg ggaaaaacaa ggtttgttaa gggtatgtga
     7021 gggaaataaa ataaatccat atgacatttt gttatggttt agagaaatta gggaagaata
     7081 cgatatttat attccctgga ttggatatga cccttggcac gttgactcga gtttgttttt
     7141 agcttatgag aatgagtttg gaaaggattc tatgattaag gttcgacaag gggtttatac
     7201 tttatctgcc cctatgaaag agctgcgagc tgatttaaaa gctaataaag ttatttataa
     7261 taataacccg atcgataaat ggtgtcttag caatatggaa ataaaaacgg atataaatgg
     7321 aaatatacag ccaataaaag gtatggacag aagacgacgc attgatggag gtgtcacttt
     7381 aattataggc tatgttgttc taaaagaaaa aatggcagaa tatgaaaaca tgatttagat
     7441 aagggggtga aaaatgaata tatttaaatc taagaagaaa aataaagaag ctcctggaaa
     7501 agttgtgatg gagcttgttt cagattcggg aaacgggttt tatagttggc atggcaattt
     7561 atatagaagt gacattataa gaagtattat acgaccaaaa agtaaagctg ttggcaaaat
     7621 gacagctaag catattagaa gtaatgagac tgaatttaag accaatccgg aaatttacat
     7681 taaatttttg cttgaaaatc cgaatccatt catgagtggg caaatacttc aagagaaaat
     7741 ggttactcag ttagaactta atagtaatgc ttttgctgta attattaaag atagttataa
     7801 tataccaatt cagatttacc ctcttaacgc tttaaatgtt gaagctattt atgaagatga
     7861 agttttgttt ttgaaatttt cgcttaagaa tgggaaaaaa gttacttatc catattcgaa
     7921 cataattcat ctaagaaaag attttaacga aaatgattta tttggaacac ctccaactaa
     7981 agtgcttgag ccgcttatgg aagttgtaaa cacgacagac caaggggtcg taaaagctat
     8041 taaaaatagc aatacaatta agtggttact aaaatttaaa acagcactta gacctgatga
     8101 cataaggaaa gaagtcaagg agtttgaaaa gaattacttg caaatagact cagacgcagg
     8161 cggagctgct gcaactgatt ctaaatatga tgctgaacag gttaaagctg aaagttatgt
     8221 tcctaacgca gcacaaatgg ataaagctgt acaacgttta tattcgtttt ttaatacgaa
     8281 tgagaaaata attcaaagca agtatacaga agacgaatgg agggcttatt atgagtctga
     8341 aattgaacca gtcgggttgc aattgtctaa tcagtataca gaaaaacttt ttactaaaaa
     8401 agagcgaagc tttggaaata aaataatatt tgaagcttct aatctgcaat atgcgagtat
     8461 gtctactaaa ttaaatttag tgcaaatggt tgacaggggt tcacttactc cgaatgagtg
     8521 gcgtaaaata atgaaccttt cgccaataga aaatggcgac aaaccaattc gtagattgga
     8581 tactgcgact gtgaaagggg gtgagtaaga taaatggcta ataataattt aaatgagttt
     8641 ttgaaaatta aaaattcaac agaaacaagt tcagatttgt atttttacgg agacattgtt
     8701 tgtgatgaat gggatgcttg ggcagaagaa gaccaatacc cgctttcaat aaaaaatttt
     8761 ttagcaaccg agcagggaaa agatttaaat atctatataa atagcggcgg agggtctgta
     8821 tttgcgggta tggcaatata taacatgcta aaaaggcatg aaggatttaa aactgtttat
     8881 gtagatggcg tagctgcaag tattgctagc gttatagctt tagcaggaga caaagttgta
     8941 atccccgaaa atgcttattt tatgattcat aagccttgga taggactatg gggcgcatat
     9001 aactcagaca aattaattaa ggcatcacaa gatttagata gaatagagga aggtatttta
     9061 aatgtatatc aagataactt aaaagaagga attgatattg aagaaataaa agaaaagcta
     9121 aatgaagaaa cttggttcac tggtaaagaa gcatcaaatt attttaattt tgaagttgat
     9181 gagaaaaaaa aggttgccgc ttgtgtgagc gattatttca ctaagtataa taaaatacct
     9241 tatgctttaa aaaataaaac agataagatt agtattggaa aagaaaataa taataaaaaa
     9301 agagttcaga tgaaactgaa cttattaaaa ttaggaggtt taaatgacta gagaagagta
     9361 ttttgagaaa agacaagaaa tgatagacga agcacaaaaa ttacttgatg atgagattgg
     9421 agaagaagga acaggagaag gaaagacgga agaagctgaa aaaatagcta ataagataaa
     9481 agcattagac gaggaatatg aaagaaatgt aaaggctagg gcgaatttaa gagcgttgca
     9541 ggacgatttt aaagtcaacc ctactatttt taatttaact aataacaaag aaaaaataga
     9601 agggatagaa gatgctatta ttgaagacga gcaagaaaaa tatgaaaatg cttggattaa
     9661 aaaaatgcaa aatagaacac tggatttaaa tgaaaatgaa gtattatcta aagtaaatat
     9721 gaaatataga aatgaaataa gaacaacaaa tagtgatgct atattgattc cggaaactgt
     9781 ttcaaaaggt atttggactg aaattggaaa cttgtatcct ttgtttggag atacttctcc
     9841 gactttcgta aaaggtgatt ttgtaattat caaagaagaa aatagtggtg atgatgctag
     9901 ttggtatgat gaggcaacac ctattaaaga agatggctat actcttggcg aaataacttt
     9961 aagagggtgt gagcttgcaa aagatataac aacatctttc aagatagaaa atatggcaaa
    10021 tgaggatttc gtaccatata taagtaaact tttggctgaa aagatgggtg ctgctctagc
    10081 taaagcgatt tatagtggaa aagggaagcc gggttcaagt gaatcttttg tagcagagcc
    10141 aagaggtatt aaaacagctt tgatggcgga agttgaaaag ccgcaaataa ttgaatacac
    10201 tgatagcatt ggttactctg atttaactaa gttgatggct gtcttaggaa aatggggtaa
    10261 tggagcttgt atctatgcta ataacacaac tatttggact caactggcag aaataaaaga
    10321 ctcaatgggt agacctttgt ttattccaga tatggttaat actgagggag taggtagaat
    10381 tttaggaaaa gtggttaaag cagatgatag cataaatgat ggtgaaatta tagctgggaa
    10441 tgtttcaaga ggatatgcta taaatataaa taaagatgtt gctttagact ctcaaagaga
    10501 taagaaaaaa agaactatag attttatatc atattgcata gtagatggag atgttataag
    10561 taataaagcc tttggcatga tagtaaaaaa aactagtgca attgcgaagt aggtgttttg
    10621 aatgattgta acattagaag aaataaaaga atatttaaga ttagaagcag attatgaaga
    10681 agatgataat ctgcttttat cttttttaaa agcagcggaa gaagacttag aaaaccgaac
    10741 aggaaaagtt tttaatgaaa ataacaactc tgaacttgtt agtttgtata taaaaatgta
    10801 tgttgcagag caatatgaaa aaagaggggc aacagaaggt aatagcgaaa aagttagatt
    10861 tgttttagaa agtataatat ctcaaatttc tatatgtagt aggtattaaa aaatggatat
    10921 tggaaaatta actcaaagaa tagatataca aatttatgga gaaattgaaa acgacatagg
    10981 agaggttaca aagggatggg ctacttataa aaaactttgg gctaacaaat cgttgcttag
    11041 aaatagtaat aattatgtgt tagataaaga aaatatagag tattcttaca gatttaaaat
    11101 aagatataga actgatataa cagaagctat gagaatagtt tgtaatgatg ttgtctatga
    11161 tataaagcat gtaaataata taaaagagct aaataaatat gaaacaaata ttgattgtat
    11221 tctttataaa gaaggtgttt acaatgagta atacagattt taatatgaat ggattagatg
    11281 attatacaaa taaactattt aagcgtattg taaaagaata ccccaagaaa gctgaaaagc
    11341 ttatgaatat cagcttagga aaatgcaaag gagaagctat tgcaagaact ccgaagtcag
    11401 ataaaaagcc taaaaaatac aaaagagcta agcacatgaa agataattgg aaaactaaaa
    11461 cgcagtctaa aaatgggaat tttgtaggtg ttttaaaaaa taattctcca catgcgtatc
    11521 taatagaaaa tggctgggta acaaaaaatg gaggctatgt cgaaggaaaa catatgttgc
    11581 aacaaacgat ggagcatcag agagcgaaaa ttgataaaag aatagaaaaa atggttgatg
    11641 aaacttttaa tctttagagg gggtaagagt gttaaaaatt gtttctgtaa aaaaagctat
    11701 agttgaaaag cttaagtctt taaatataaa aatagtagca aatgaaataa ggagcgggtt
    11761 tgaaaaacct gcttttttta ttcaaatcat tcctattgaa atggctagcg acccaagttt
    11821 ttcaaatagt acattacttg ttaatataca ttatttttct aaagaaaaga cagaattaga
    11881 aaatttaaaa atgattgata agcttaatat attatttcaa gattgtattt tagaaattga
    11941 agggggcaaa ttgactatag aagaaaaaag tgtagaaata tatgagaatg ttttacaata
    12001 caaatttaat ttgcaagtag ttgaaattat agaagaagac gaaagtaaat atgagcttat
    12061 gaaagagctt gaaatgaata tttaaaaaag gaggttttat tttgggatta ccaagtgcga
    12121 taattgagtt tcaaagacgt tcaaggactg ttaaatttag aagtcgaaga ggtattgtag
    12181 ctttaatact taaagattca acagctataa agaaatctta ttctatcgat tttttaacgg
    12241 atataaatga aactgaattt acaaaagaaa attatgatta tataaggctt gcatttttag
    12301 gaaaacctag caaggttatt attgaagtta ttaatgattc agttgattct gaaaggtctt
    12361 tagacgatgc tttgaaagct ttaagggaga ataaatttaa ctatttagct attcctttta
    12421 taagtgaaga agttgacaaa actaaaatag ttaattggat aaaaacagct agaagggaaa
    12481 aagagatata caaagctgtg ttgccaagcg ttactaatgc taacgagaag gcaattataa
    12541 acttctcgac aacaggaata aaagttggcg aaaaagttta tacaacagca gaatacacag
    12601 ctagagtcgc aggcattttg gcgggcatat cgctttcaga gagctgcaca tattttgttt
    12661 tagatgaagt tacagagata gaacctactg aaaatcctga cgaagctgta gacgaaggaa
    12721 aactaatttt aataaataat aatgggataa ggatagctag gggcgtaaac tcattaataa
    12781 ctttaagcaa agaggataca gaagacttga agaaaataaa aatagttgaa gctattgata
    12841 tgatacaaga tgatattctt caaacctgga acgaaaatta tgttggtaaa gtaactaata
    12901 aatatgataa taaaatattg tttttatctg ctgtaaacaa ttattttaaa gagttgcaac
    12961 gtgatgaagt acttgacaac agccaagaag cttatgcaca gatagatata gaagcgcata
    13021 aaaaatatct aaaagaaaaa ggcattgatt atagcgaaat gactgaacag caaataaaag
    13081 aagctaatac aggttcttat gtttttatag aaggaaacat cactgttact gatgctatgg
    13141 aagacttgaa atttaaaata tatatgtaaa gaaggtgaat agatgggcaa agaaaatatc
    13201 gtaggaagta gtcaaatttc cggcacatgg ggaaagcttt ggtgggatgg aactttaatt
    13261 gctgaggtgc ttagttttga agctaaagtt acagcaaata gagaagagat tcagtttgga
    13321 atgagtaaag attctaaaat aacatcgcta agcggcgaag ggactgtaaa acttggaaaa
    13381 gtgtattcca gggggaagaa aaaattattg gaagcttgga aaaatggaga agacccacga
    13441 agcacactta caagtaaggt gaaagaccct ggcacacctg ggaaacaagc agaaacagtc
    13501 acaattaaca acgtgtggtt taatgaacta gctttatcac agtttgaaaa gggcggaaaa
    13561 atcgaagaag agctaagctt tggatttaca ccaaacgatt cagacgtgat ggacgaaata
    13621 gacgaaattt aaaggatagt ttttactatc cttttttata taataggagg attttaaaat
    13681 ggataataaa aaagaaatgg taacaataga ggatatttta agaagaaaag aatattttgc
    13741 aaagaaaagt gaagaaacca agcaattata tattccttcg cttgatggaa atatagagat
    13801 ttcaaagccg gacaggatgt tgtgtcttga tgcaatagaa atggaagacg cagtcgaagg
    13861 agataaatat tttgtatatg aaattgttaa aagtccgaat ttaaagagcg aaaaattgca
    13921 tgctgaattt ggatgtaaag ataacccact tgatatagtc gatgtattat ttgaagcagg
    13981 tgaaattact gatattgtca agattgcaac aaagtttgca gggtttgggg ttgtagagga
    14041 aattgaagac ttaaaaaact aattaaaagc gatgtggaaa tgcaaatgat tagtcattat
    14101 ctagaaaaag gtgtcgattt agataaatta actaatttaa atatgataga aaaaaaattt
    14161 cacatcgctt gtatgctata tgaagaagaa gaaaaaataa aacttatttc tgagctaata
    14221 ggtgctatgt ttggaggtgt aaaaaatggc tagaaggcat ataggtgcag ttatatctct
    14281 aaaagacaat atgagtgcta ccatgagggg gattagaaga gagcaaaaac aatttcaaaa
    14341 cgaggttaga cggacacgta acgagatgcg ttcggcaagt agagagcgca tgcgcataag
    14401 gatggatgca actcctgcac acaggactat acaagattta aggcgaaaat ttgcacctct
    14461 tcgtactaaa ctcgtaaaag ctgttgttat aaaagattta gcaactgaaa agatagaaag
    14521 aataaaatcg aacgtaaaat cttttggaaa atttattgca agacctgtcg taaaacttaa
    14581 agatgaaaca aaaggaatga ttgataaaat aaaaaatcgg cttactagtt tatcaactat
    14641 agttccagtt ggtgctgcag ttggcgctgc gggtatggct gttaaaagtg gtatggaact
    14701 agagcaacaa caaataagta tgcgtcattt catgggagtt ggaaacaaag gaaagtctag
    14761 caaagagctt gatggaatga gcgcaagcta tttaaaagat ttaagaaata atgcaaatgc
    14821 gacaccattt gaaactggag aagttatatc agcaggaact cgttctttgc aaatagcggg
    14881 tggaaataca aaagatgcta tgcagatggt taaactagca gaggacatgg cggcgctaaa
    14941 tccaggcaag accgttggag atgctatgga agcacttgcg gacatgaaca ttggagaaat
    15001 ggcaagactt acggagtttg gagttaaggc aagcagtaca gacgacccaa aggaagtaca
    15061 aaagaaactt gaaacaatgt atgcaggagg agcaaataag ctcgctgaaa gtggttcggg
    15121 acttctttct acgataatgg gtaagttaaa gtctaatatc gcagacattg ggcttggcat
    15181 gttagaacct ctcaaaccgg ttatgactgg cttaattgga tttatagacc aagctagtcc
    15241 taagatatta gaggtaggta caaaaataac aagtggaata ggtatggcga ttggatggat
    15301 acagcaacaa atgccaactt tagctccaat ttttcaaaca gcttttggag ctgtatcttc
    15361 gattgtatca actgttgccc caataattgg tcaagttata ggagctttaa gccctgtttt
    15421 tatgggattg ctttctgttg cttcttctgc attgtcaggg attgcttctg ctgttaaaac
    15481 tgtagctcct gttgtaagtt ctttaatttc taagttagcg ccaatttttt taaatgttgg
    15541 aagtacttta aaatctatgg gtaagatttt taaaaatgtt tttgatagtg ttatgaaaat
    15601 agttaaaaaa gcgtctgatt tcataaaacc attagtcaat ggaataatag gtgcaacaaa
    15661 aggcattagt gatggagtta gttgggttgc tggaaaactg gctggaaatg caactggaac
    15721 gaaatattgg tcgggcggac tttctgtcgt aggcgaacat ggacccgaac ttgtatctat
    15781 gccacgtggt agcaaggttt ttacaaatgc agaaagtaag tctatgatta ataaaagtat
    15841 tccaaatttt agacaagtgc aaggagaaaa cacaaattat aatataacaa ttcctaaaat
    15901 tgctgaacaa atagtaataa gagaagatgc tgacattgaa agaataacat caagcttaat
    15961 aaaaaaaata caaatggcga aaatgggcgg tgttgtttaa tggaaatgtg gcttagacaa
    16021 tcgaatgatt cttttagatt tcctatactt ccgacctctt ttgaaataag cggaaatata
    16081 aatacaagta caacaaatgt gttgaaactt ggagaagtaa ttgtttgtgg gggtatggga
    16141 cttagaacaa cagagataaa cagttttttt ccaggtgaac aatatcattt ttgcaattat
    16201 aaagattttc cacaaccata tgattgtgta aataaattga aaaagtggat ggagcagggg
    16261 ttaattttaa ggtatataat aactgaaact gatgtgaata tggaggttat tattgaaagc
    16321 ttcaaacatg gcaagcagga tggtacaaac gatgtttact ttacattgag tttaaaagaa
    16381 tataaaagaa ttcagatacc taaagtaaat actaataatg atgaaaagtt atcttctgta
    16441 aaagatgtgc cactgacaaa aggatttgag gttaagaaac aaagaactca taaggtaagt
    16501 aaaggtgaca gcctttggag tttggcaaaa aaatattatg gtaatgggga tttgtggaag
    16561 aagatttatg atgcaaataa aaaattaatt aaaaatcctg acatcatcca agatggttgg
    16621 gtattggtaa ttccataggc tggaggtgat ttataattaa caatataaag ttaaaagtac
    16681 acataaaaaa tggcaatatc tatgatataa ctgatatagt agaaaaagtt acatggagtg
    16741 gtgattataa gtcaccatca aggacattag aattttctat agtacagtca gcttctgata
    16801 ttaattttag aaagatagat atacctatag ctagtacagt ttgtttttat gtggatgata
    16861 aagaactcta tagaggaatg ataattaata ggtctaaaga ctctagcaac aatagtatta
    16921 gttttgtatc taaagatatg ggattcttgt taacacagag tgaagtgtca tataacttta
    16981 aagataaatt agttgaagat attgcaaaac aggtatttaa tgacaataaa cttgcaatag
    17041 gaaatatacc taaaactaat gttaaataca ctaagatgtt tattggtgta actggctatg
    17101 atactataat gagtgcatat acagaagcta gtaagacaac gaagaaaaag tatatgatag
    17161 agtctaattt agataaattt aatgttattg aaaaagggat tattacacta aatgttatgt
    17221 ttgaagaagg gtctaatctt attaacacga gcttttcaga gagtatggag aatgtaaaaa
    17281 acaaggtatt agttgtagac cagtatggaa ataaaataag tgaaaagata gacgataaaa
    17341 tttttaaaga tgttggagta ataatgcaaa aggttataca gcaacaagaa aatagtactg
    17401 tagatattga aagtgagttc aagggaatag aacagacttg cagtttgaaa gggtatggtg
    17461 acgtgacttg tataactggc agaggtgtaa aagttaagga tagttacaca ggtcttacag
    17521 ggctatttta tatagacaca gataagcata attgggacag taacggaaat tatgagatag
    17581 atttagattt aaattttcaa aatatcatgg atgaaaagac agcaggacag gacgaacaga
    17641 aggaagaaag ttcagatttg aatggagaag gtacactaaa tggaagagaa gtaaaagcag
    17701 aatttacagc ttattatccg tctaataacc ctatggaggg tggttattat caagctatgg
    17761 atggtaaaag acttgtacct tcaaacaata cttgtgctgc acctagtaaa cttaaattta
    17821 aaacaaaaat tcaagcaaaa tgtcctggaa ctaaaattga tggtaaaact tatacagtaa
    17881 cagacagagg aggagcgatt ggcttaaaaa acggagtgta tagaatagat atattaatgt
    17941 ctagtgaaaa agaatgtaat gattttggaa gaagaaaagg aacaataata attggagatg
    18001 gcacaggcta tacaaatgca acaggaaaag ctaaagaatt gataagtata gcaaaaagca
    18061 agctaggctg taagtatgtt tggggagcta ctggtccaaa tacttttgat tgtagtggat
    18121 tcactcaatg gtgttacaaa aagataggta taaacattcc tagagtatca agagaccagg
    18181 gaaaggcagg taaagcagta agtaaaggaa gtttacaact aggagattta gttttctttt
    18241 ctagtaaagg agcaaatgga gcaatagacc acgttggcat gtttattgga agtggcgaat
    18301 ttattcattc tccacatact ggtgatgttg taaaaataag taagttaagt ggtagttatt
    18361 acgctaaaaa ttatgtaaca gctagaaggt atttataaaa aggtggtgat atagtggcta
    18421 atccaataaa tgaatttata ggaataataa gagaggaagg aaaacattac aatgaacctt
    18481 ctttttttat tggggaaatt aaaagtaaat taccagattt aaaaatagag gtaaataatg
    18541 tcatattaga aaaagaagat attttaatag atagttggat tcttgatagg cagatagaaa
    18601 catttaatac agaaacaagc caggaacata ggcatgaagt aaaaaatccg ttcatagata
    18661 cctttgagga agaggacaca gtaataatgt ttaagatagg tgataaattt gctgttgtaa
    18721 gtaagttggt gagcttataa tgagtacaat atttcctttc ataggtgtac cagaagatta
    18781 tgaaataccg aaaacagaag aattagaatt atttaaagaa gtggcctggg attttgaaaa
    18841 agatgaacca gttgtggaaa atggagattt taaggtcatt gagggcaatg aagctataaa
    18901 agtttgggtg tataaatgta taaaaactaa caagtatgag catgagattt attcatgggg
    18961 ttatggaaca gaactttcag agcttatagg tcaaaaatac agcaaaggac ttacagaaag
    19021 tgaagctagt agatatataa aagaggcttt attagttaat ccatatatat tagatatcaa
    19081 tgtcaagaat acaaaattta cagatgattt attaagtgta gatatagaaa tttcaacaat
    19141 ttatggggag gtggaagtta atgtatagta gtcaaacata tgacgttatt aaaaatagaa
    19201 ctctatccaa catagattta gatatctata aaagtgaggg ttctttttta agtgatatgg
    19261 tatctccaat cagtacagaa ctcgcaaaat tctatataga actttcatat cttcataaaa
    19321 aagcttttat agaagataac ttcgacgatt ttttagataa gagagttaac gaatttggag
    19381 tatatagaaa gttaggaacg gaagcaactg gagaggtaat atttgaagga aaagctggaa
    19441 caattgttca aaacggaaca attatatctt acaatgagct attattcgta gtaattaaag
    19501 atatagtaat tagttcagaa atcgaacaaa atacaagccc cgtacaggct ttagaaattg
    19561 gaattagata taatatacct gcaaacactg aatttaagct acaagacgaa ataaacggta
    19621 taacgaaaat ttataataat ttagacttta aaggtggtac agaaatagaa acagacgaag
    19681 aattaaaaga gagattctat aaaatacaga aaaatcaagc cacaagtgga aataaagcgc
    19741 actatgaagc atgggcttta gaagttgaag gagtatataa cgctaaaatt tatccaagat
    19801 gggatggacc aggaacggta aaagttttaa tctttggaga aaataatcaa gctgttgaca
    19861 cagaagtaat tgaaagatgc aaagaacata ttgagataga aatgccaata ggtcctgcat
    19921 taacagttct aactccaact attttagata taaatataag tgcatctata aaactagaag
    19981 caggttatac actagatttt gtaaaagaaa gcttcttaga aagcattaat agttacttaa
    20041 taaatgttaa taaagaaata atttatacta aagtaagtgc aatacttgca agtacagaag
    20101 gtttacatga ctttagtaat ttattattaa ataataaagc tggaaatata gtatttgaag
    20161 aagacaaagt accaagtgta actactctaa attttagtga ggtggtttaa ttgaaattaa
    20221 ttgataaact accttctttt tacaacaatg atgtaaccag aaaaatacaa gaagcttatg
    20281 acatagagct agaaacactt agagaaacat atgacgatac ctttgatcaa ttttttgttg
    20341 acacagcaac gtgggggctt gattattggg aaaatatttt atctattaaa aatagatttg
    20401 atttaagtat agaagataga agaagtaata taaaagcaaa aatgagaggt aagggcacaa
    20461 ctacaataga agttataaaa gctatatcag aagcttatac aaagactaat gttgatgtag
    20521 aagtatttag taatctattt agttttacac ttagttttat aacaaatgat tgtagttata
    20581 acactatttt agaattagat aagaaaatag aagaaataaa accatgtcat cttgaacaca
    20641 aatttgaaat gattttattt aatcaaaata acatgtttac aggttctaca atgaatacag
    20701 gtgaaacagt tactatatat ccttacagtc caactgatat aacttctttt ggagaattga
    20761 ctataactac aggtaatgac agaagtatgg aaaaagtaac attatatcct agacaggagg
    20821 tggtataggt tggcagaaca aaaatattat acacttttga caacgatagg aaaggcatct
    20881 attgcaaatg cgacagcttt aggcaataaa gtagatttag ttaaattaca gcttggtgac
    20941 ggagcagggg cagaatataa tccaacggaa gaacaaactt ctctaaaaaa cgtagtttgg
    21001 gaaggttcta taaataatgt aaagatagac gaagaaaatc caaactggat agtaatagaa
    21061 actgtaatcc caggtagtgt gggtggcttt atgataagag aagtagggat atttgattca
    21121 aaagaacaac taattgcagt atctaaatat ccagaaacgt acaaaccaac tgctgactcg
    21181 gggagtgtaa aagacttagt tataaaaatt attttagtag tttctaatac ttcaagtgta
    21241 aatttgaaag tagacccaac agtaatttta gcaactttaa aagacataca agacttagac
    21301 actaaaattg atacaactaa aacagaatta acaagcaaca tagaaactgc taaaacagag
    21361 ctgaacacta gaatagggga tacaacacaa cttactacaa cagataaaac aaatatagtt
    21421 agtgcattaa atgaggtaaa aagtagtgta gatagcatag aaacaacagc agagaaaaca
    21481 agtataaaag atgcagataa tttatttgaa agtggcaatg tagagggagc attaaaagaa
    21541 gtgatgcaag aagtaaaagg taatagaagt agtattatat caacagtaaa taataatttg
    21601 ataccaatgt agaaagagag gtgaggatat gagacattat aaaagaacaa cagaaatgaa
    21661 aaagagaaga ggaacatatt ctgaaactga tttgtatgca tatcaacagg cgtttagtta
    21721 tactatcgaa ccagactgga attatgtagg tacctttgaa cgaagcaaaa taaaaaatat
    21781 ttctataaat caaaacacaa atatattctt aaccacaaat aatatatata aaattcgcta
    21841 ttctgataat agtaaaacaa ctgtaacaga tttagaaatg aaacaaatac ttgatattac
    21901 atttataaaa gaccctgtaa tcaatttaac cttaagtgat attagcaagg gttgtactat
    21961 agattacagt attacagata atgaacctac tcaaaaattc atagtaacag aaaaattaaa
    22021 taatactgtt atagcaacaa aactcaattc tattggtggc aactatagca ttgtacttac
    22081 agatgaacag atattgccac tacaagtaaa ctcgcaaaat aatatcacta tagaaattag
    22141 tacagaacaa ggcggattag ttgtaagtaa gacagcaaca tttacaagaa taaataataa
    22201 accaactgtt ttagttagtt catataattc taattctgca aaatttacag catctgattt
    22261 agataataat ttaagtaaaa ttgagtggta tttagatgat gtattaaaag aaacaataac
    22321 aacagattta acagcagaga aaacaataaa ctatgaactt acagacaatg caatacacac
    22381 attaaaaata gttgctacag atgcagaaaa tgcaacagtt gagaaagttt taagtataag
    22441 taaagaaata atgccactgc aaacagacgc tagtttaagc gatatatcaa ctaagttgat
    22501 agagattgga gaagggttta agaatgggaa aacaagtatt ataaatactt tagcattaaa
    22561 gaacatagaa gcaagtttga ataacacact tgttgagtta tcagagaaaa taaaaacaag
    22621 ttttgatagt tcagatgcta gtgtgcaaga tttgatgaat cagttaacac aagctaataa
    22681 tactatatca cagttaaatt ctaagtataa atatgccagt ggtactattg atgttgttaa
    22741 aaatagttct ttaatggcta atttatatgg agagtccttt ggtagacaac ctggtacttg
    22801 gcttaagatt gataatttag gttttattcc taatattttt gttgctgaat gtcaatatgt
    22861 tacttctaat aattattttt ttaaacatat tgttattgct acttgtaata taaattggct
    22921 ttgggaaaaa aaagattttt cggctagaat tgtatttact aaagaaaaaa aatctaatca
    22981 agattttagt ggaagtggaa ttatttattc aaataatgag cgtgatgtat atataaataa
    23041 taaaggcatt aatgttcctg caagcagtcc caatgcttct gactacttac attcttggca
    23101 tgctataaaa tttatttaat aaatgaggtg ataaaatgaa tagagcaaat agaattatat
    23161 gtgaccaaac aggaaaaata ttactccaaa caggagaagc aacaggggat atattagagc
    23221 atgatgaaat aactgaatta cattttgttg atattccgta tggaagtgta gattatacta
    23281 aaagcagaat tgtaggcata aatatagaaa caaaagagcc aattttagaa gaaatgccag
    23341 tatatctaac agaagaagaa aagagaatac aagagttaga aaatcaatta ctaattgcag
    23401 aaaatgaaaa ggtaggagga attttataat gaatataaat aatgttgtag taagaatatt
    23461 ggcagagaga atattaaaca aaggattgaa ccctttgaaa aatcgagaat ttgaattaga
    23521 cgatgtgact aacacagagt acagaaaagc agtagaggat tatataatag aacatagtgg
    23581 agtagtagaa ggaacagaac ctacaaaata ggttcttttt taatacaaag cattaggagg
    23641 cttacatgaa tgaagaactt ttagaagcag atttaaaaag acatgaaaca agaataaata
    23701 agcatggaga agaaatagac gaattaaaga tagcaaatat agagtctaaa gcagagttaa
    23761 aagcactatg tgagaactta aactcactta caagtatgtt aaagtggttg attggaacaa
    23821 tgattacaac cttggtaggg ttctttatat ttgcaataca aaaaggaata ttttaatcag
    23881 gaggataaaa aatggataat ttgataagtt ttataccaga gcaattactt ttattggtag
    23941 ttgctcttaa tgtattagga tttggattta agaaatacaa acagttagat aataaataca
    24001 ttccaattat attgcttgtg ctaggaatag tattttcaat atggatgcta ggatttaacc
    24061 cttcatcaat tttacaaggt attttatgtt ggggagtagc cataggagca aatcaagttt
    24121 acaaacaact aaaggaggaa aataaataat gaaaatagca atagtaccag gacacacttt
    24181 aagtggaaaa ggaacaggag cagtaggtta tatagatgaa ggaaaagaaa atagaatttt
    24241 aactgattta attgttaaat ggttgaaaca aggtggagct actgtatata ctggaaaggt
    24301 agataagtcc aataactatt tagcagagca atgtcaaata gccaataagc aaaatgtaga
    24361 cttagcagta caaatccact ttaatgcgaa cagtacaacc ctaaatccaa tgggtacaga
    24421 aacaatatat aaaactaaca atggcaaagt atatgctgaa agagttaatg ataaattagc
    24481 aacagtattt aaaaacagag gtgcaaaatc agacgtaaga ggactttact ggcttagtca
    24541 tacaaaagct ccagcaatat taatagaagt gtgcttcgta gatagcaaag cagatacaga
    24601 ttattatatc agacataaag acatagttgc taaattaata gcagaaggta tattaaataa
    24661 aaatataaat aatgagggag ttaaacagat gtacaaacat acaatagttt atgatggaga
    24721 agttgacaaa atccctgcaa ctgtggttgg ttggggttat aatgatggga aaatactgat
    24781 atgtgatata aaagattacg taccaggtca gacgcaaaat ctttatgttg taggcggtgg
    24841 agcatgtgag aagataggtt ctattactaa agaaaattat actatgataa aaggtaatga
    24901 tagatttgat accctttata aggcattaga ttttattaag aaataagttt gcagagagtg
    24961 gtaactattt catcagttac cactctctat ttttttatcc tttttgttat attctctttc
    25021 aagtgctgtt tttttaaaaa cccatgtcgt tccgaatttt ttacagtcta caccttccac
    25081 aaattttcca tttcttatat taagtcttaa cgtactttca ccttttttat aaagttcaca
    25141 agcatctttt atagagatta gttcctcaaa tacatctttc attttaacaa ctccttttat
    25201 ttatattata atcgcaaaag tttaaaaaga caagaaaaaa ataaacgttt atgtttaaaa
    25261 aactattgac ttaataaacg taagcgttta taatatgatt aaagaaagat aaacaaaggg
    25321 ggatttaaaa atgaaaaaaa taactaagaa agaaattaaa aagtacgtta gagaagcggt
    25381 taataacaat tttaactggg aaataaataa atgtgggttt tacattaaaa ataatgaaat
    25441 agaatttttt atttcttaca agggtcaagg aatagatgaa aatgtatata atgatactta
    25501 tgaagaaata atttacattg aggatattat agaagaatac agaagaaaag aatacaattt
    25561 agaagatgta gattctattg tatatgacaa tgtaaatgat atgattaatt gttataatga
    25621 agaaaagtaa taaaaataaa tacagtgagt ttaatcaaat tcactgtatt tatttttaca
    25681 atagtagaat aatattatta gttcaggatg tgataggctt gaaaattgga gataaattcg
    25741 aaaaccttac aatactagac atagaacaaa aaaacagtag gaaatactgt ctatgtaaat
    25801 gtaaaaattg tggcaatgaa aagtggataa gagcagatag tttaaaaaga attaaagggt
    25861 gtggatgttt gcaaaaagaa acacagttta agcaaaatga tttgacaaat aaaaagtttg
    25921 gaagattaac agcactaaaa aacactaata aaaaagctaa aagtggtcac tatatttgga
    25981 tttgtaagtg ctcttgtgga aatgagatta agacaacaga aaacaattta actactggca
    26041 gaactaaatc atgtggatgt ttgaaaaagg aatctaatat aaaaaatgca aagatagcat
    26101 taaaagtaca taaagaaaaa aacattattg atgatacaaa tctatctatt ataaaaaaga
    26161 cagaagcata tcctaattca aaaactaaaa ttagaggagt ttcttggaat aaagaaaaaa
    26221 gaaaatactg cgcacagata gaatttaaga aaacacatta taatttagga tactatgaca
    26281 acataagaga agcagaagaa gcatacaaaa aagccaaaga aaaattctta aaagaaataa
    26341 acggaaaagt ttaaaaaacc attgacttaa taaacgcaaa cgtttataat ataattaaag
    26401 aaagagataa tcaagaggag gatataaaaa atgaataaac aaaaagctag aagattttta
    26461 agagttatag atatgaatat agataaaata gaggaagaag ctataaaagc ttttaaagaa
    26521 agttgtttaa tcaaagagac taataatata aaaatttata tcgatataca aggaaaagtt
    26581 gaagcgatag cagttcaaac ttgggctaaa cttttagatg atgacaaaga aattaatatt
    26641 ttcacattaa atcaagcgcc aactcattta gacgatatgt ttggagaaat ttaccatgta
    26701 aatgattatg aagagttcga aaaatggtgt gaaaaggaag ataaaagttt gaattgggat
    26761 agttatgaaa aattcaataa agaaaatttc gaagaaatcg ctgaaagaaa tatagatgat
    26821 agctcatcag cttttttaga aaaattacgt gaaggcattg aaaattgtaa acaagaatta
    26881 caaaatatag ttgaaaatta agtagaagag tattattatt aatgtatata gtatcgcttt
    26941 tggttaacta taagggattt aaaatatttt ttaatttatt tagaaatgat attgtaataa
    27001 gaattttcag attaactatg agggatttaa atgtcaattt aatttataaa gaaataatat
    27061 aatatcacat tatttttaga ttaactatgc ggaaataaaa gcagtgtatt caataaatgc
    27121 actgcttttt taatgctaaa gatttaatta ttatgataac tcaatccttc gattaaaaat
    27181 cctacgttca ttgtcatttc tgcttcgtcg ctttgcaata ttccttccgt aaattcgctg
    27241 tcgcttggta atgatacttt ttttcttgca actgcttcta tgattgtttg aaatagttta
    27301 attttcatat ccttatcaaa tccagcgtat cgcaaaaaat ttacaacttc ctgtgaaaac
    27361 ttatttaatt cagatttagt cattttttca gataactctt ttccaatttg caaataaaca
    27421 ttcatctttt ccaacccctt tttagattat agttaaaaca tattatttaa acaatattga
    27481 tatattgtaa taataatatt aaaatattac ttaaatatta tatatacaat atttaagtaa
    27541 tatctaaaca atataaatat aatattattt actttttctt agtcttattt cttctatttc
    27601 tctcattgta tatatttaaa ttctcttctt ttatagtgac atcagataaa atttgagtta
    27661 gtatatcact taataattta ctaacgtcgc tgttcatatc aatagaaaaa gattttaatt
    27721 tgtttacaag ttcttcatca agcgttgcac tcatagtcac ttttttactt ttcttattaa
    27781 ctaaattata tatgtttaat gtcttattgt ctgtagttgt agaatcattt tgtgcgattg
    27841 ttccattttt caaaatcggt ttttccttat caactaaaag gtctctgttg aaacttggca
    27901 tctataacac tccttttctt ttcaattctt ctaacatatt agtaaattcc tgtctgctga
    27961 aatgttcttt agttattttt gtataatctg ttagagatag tttttttaat agtgccttct
    28021 ctataaactc attttttcta atgtatgtat ctaacataat atctctcata tcgttaaacc
    28081 cttccagata agaatcgaaa gtatcgctaa tttgagtttt cttcttctca aatccaacta
    28141 aaatagtact ttttatatta tcctccttat caaaataggt tctatcaaca tcccagagtt
    28201 gtttaaataa ttctgctcct cttaaagaag aaatgttttt atcttgaatt ggaataatta
    28261 tactatctgc taagaaaagc acatttttag ctgtcaaatc gtagcgtgga gataaatcac
    28321 agataatata atcatactca ctcaaagtat taaagttttg catgtaccag cgagctaaaa
    28381 atttttctct ggctggtaaa ggtgatagct cttgttcaaa tctagccatt tgtatgtcag
    28441 aaggaattaa gtctacatta atgtaatttt cattaggtga cttgactatt gcatcattag
    28501 cactaatccc ttttaaaata tctaatgtag tattatcatt atgatttatt tcataaacat
    28561 attgtgttag atttgcttgt acatctaagt cccataagag tattttttta tctttatctt
    28621 tcgcaagttc atacgctgtc attatagacg ttgttgtctt atagatacct ccttttatgt
    28681 taaagtacgt aagtagcttt gtattagcca ttttctccac ctcattatta tatttataat
    28741 attataataa tattgtagta ttgtttttat aatattgcaa caatatatta atattgttaa
    28801 ttacaatatt caaataatat aaatacaatt aaagtaaaaa attatctaga ctttttgaat
    28861 ttgattttta aatcaaattc ccaaccactc ttagtcgcgt ttgagtggtt ttgtgttttt
    28921 aaactaatta acatatatga agtgatgtaa aattgtatct ttttttaaaa aatgtgatat
    28981 aataaaagca aggaaataat gatttacctt acacaagagt agctatttcc aatgctttga
    29041 aaaataactt attttcttaa accactctta gtcgcgtttg agtggttttt tattttatca
    29101 tatatgaaag ctgatataac accagctagt atactcaata aaaaatttat caccatactg
    29161 ttccacctcc ttccgttatg gaatttggcg tttaaatatg gaaatagcca cccttacgct
    29221 tttcaatcat tttttccttg ctaaaattat tataacatat tttaacaact aactaataaa
    29281 ctaattcatc tttaaacttc atttcgcttt tacaagcttt ataaactgat gcgcaactct
    29341 tgcaatcttc taatgctcta tgagaactat aattcaatcc taattcttcg tgcaaagaag
    29401 gcaaagaata actttttagt tttttaccct cataagttct tatgtacttt cttgacaaag
    29461 ttaatgtatc tataacctta ttagtaattt ttttatatcc attcttatat aatttatcta
    29521 atataaattc catgtcaaaa atagcgttat gagctataag agtataatca cttataaaat
    29581 tcaaaaagtg cggaagcact tcctcaatgc ttggagcatt aacaacgtct tcgtctgtta
    29641 ttcctgttat atcagttatc ttttttctta tactcttact tggcttcaca aatgtactaa
    29701 aaatttcgct tgggacatcc ctttcaaatt taatagctgc tatctcgata atatcttctt
    29761 tgaaagcatc taaaccagtt gtctctacat caaagacaat ataatgctct aaaactttat
    29821 ttttagaatt tgtaaattta ataaattttt cgtactcaat ttctttttga cttttaaaag
    29881 aactaactat gccactagaa agttctttgc taaaatcata ttgctttgca atatcaaatt
    29941 cagttagaac tggctctttt aatgttttta aaaatctttt tactgaatta aacacaaagt
    30001 catcccccct ttacagttta attataaaaa ataagactta gtttgtatac taagtcttag
    30061 taaaatttaa cattttaaaa aaatatttct aaaatttaat tgatttttta aaacagtata
    30121 tttttttata atctaaatgt gctataatta taatcataaa atgagaataa gccgattttt
    30181 ttaaattaga tttttaaaat acaaaaaaat aaaagaggaa tactcacttg ccgtcaaaca
    30241 atagtattcc tcaccatcag ggaaaatgga cccttatttt gggatacaca aatatcctta
    30301 ttttctaatt gctatattct tattatagag taaaaagcaa tagaaatcaa gtttttttag
    30361 agtattttta attagtaaaa ttctactaaa aaacagaaaa taggttttat ttttccctgt
    30421 aaaataaggg ggatttaaaa tgaaaaataa tataaataaa ttatttctag aagaatgcaa
    30481 cgctgaaagg gcagaacatg aaaaagaaaa ttattcaaat ttcaatagac atgaaacata
    30541 tgaaataaaa aaatatataa acgaaaaaaa attaggaatt aaaacggcta tagctaacgc
    30601 tttggatgga ggtaaaaccc tattcattgc gccgacggga gcaggaaaaa gttatagttt
    30661 tataaataca ttaaaaaaat taaaaactaa agcattattc atacttccaa atgcttctaa
    30721 tgtagagcaa gcaatgcacg aatacaacat agctggtgca tatgataaga tacctgctaa
    30781 acaagcttta gagaacaata atctagcagt aatgacctgg gacaagactg aacagctcat
    30841 agatgtagac ttaagagaat acataatcgt agttgatgaa atacatcaaa cttatacaga
    30901 cagctataga gggaaagcaa taaaaaattt aaacaatatc atgtctaagt gtaaaggaag
    30961 aatagatata acagcaacac caacaaaact agaatttgaa atatatgact acataataga
    31021 gtacactcaa aaacaaaaaa cagaatatga tgtcaagtta tataatgatt ttgataataa
    31081 aaattttaca gaaataatta atataataaa taagtcaaac aatagtgcca tgctaatgaa
    31141 tgatatatca actcttgagt tcataagaga ttcagtaaac aaaaatgcgg gagttgtata
    31201 tgcagatggg aaagaagaaa atgagttgta cagtagaata gttaaaaatt cagatatgaa
    31261 aggctacgaa acgcttttaa atacaacaac aatcctagct ggtgtaaata taaacaacaa
    31321 agatatcaca gatataatta ttgttaatat aaaagatatt ggagctataa agcaatatgt
    31381 tgcaaggttt agaaatttaa aaaaagtaaa tgtacacata ttcaataaat acaaagaaga
    31441 gtgtaatgtc tataaaatag aatggttggt tgataaaaat atagaaaaag caactatctt
    31501 aaaagatgct tataacaaag tatctaaaca taccctaatg tttgaaacgg ttgggataaa
    31561 tgctacacca attcgaattg attcaaatgt ttattactgt atgaaagaca attgttacaa
    31621 agtagataaa ctgtatataa aaagccaaat ttacagtaac tattacaaca caagaacaat
    31681 acaaagtttt aaagttttat tagaagaata cttcgataat gttaatataa ctgatgctaa
    31741 agaaattgaa acaaatgaaa aagaattaaa agaatataaa aaagttgtaa aagaaattaa
    31801 agaagcaaca agaaatattt taaaagaaca taaagaaatt ttggtaggtt atagacagat
    31861 taaaagcaat tctaagagct tttcactaat gcagtatcat aatgatatga aactaagttc
    31921 aaaagggtgc ttagaggctt acagagcgta tgacatacat aatttggtaa agaaaagtaa
    31981 aagtaatagc atgttagaac tatattctaa ctatgtgcta gataataaat tcagtttaga
    32041 cctcgcttgg aagttagcaa atacagcaaa tagaaagcgt ggagcaatat tcaacaagtt
    32101 aaatactttg atatacagag aattaaaaga ggaatatcca gaatttttaa atgatgaact
    32161 tattcaagtt acagtattta attatataga caaattattt ggaattggaa catcttatac
    32221 agagttgcat ttacaagagt taagtgacga cttaagattg attttaggag aaaattggaa
    32281 cttaacaact aaaaaactga gtatcatatt gaatgaagtt tttgtaatag aaaagaaaaa
    32341 atatagaggt gggaacagtt tagaaaatat ctttttttat aaaaatataa atcctaaact
    32401 gttcccactc gaagctgaac gaatgcaagt taataatata aaaagatata taagcataga
    32461 tgacataaaa aaagatttaa aattagacga aaaagacaag agcctagaac atgctataaa
    32521 acatacaaaa gatagaatgt tgaactcttt agatgaagat gaaaaactgt tgctactaaa
    32581 aggttttatg taaaaacata tggagggatg aatatgaaat taagaattgg gattatagat
    32641 aaatatgtgg ctgaggcaag ttatgagtta tcagaacttt taattgatgc ttttaataaa
    32701 tatgatgaat ttgttgaggc tataagaggt ggagaaattc gcatagtccc atttaatttg
    32761 ggatttcaag ctttgctgga agaggattct acttatgatg agtatgaatt ttattacgat
    32821 gatgaggatg ataaatgttt ttttcatatt aaatatagaa atgttaaaga tgttgaactt
    32881 tcaaaagaac ttttgctaga acttataaaa gttaatttgc ccgcaggagc aaaattaatt
    32941 gtgaacaact agaaatttgt gtttagatac aaaagccggg agtaggaata tatatatata
    33001 taaattccta ctcccggctt tttgatatga gagctgtagg gtttaaatat ttgaccctac
    33061 agtttttaaa tgtgagaaaa ttcaaagtaa attgaattgg gattattttc cactagcaaa
    33121 atttgctaaa aggtactatt attgtagtat aaatgtttaa ttacgacaaa aaatcgtaca
    33181 tgtgttcttg tgtaatcgaa caaaaaaggg tataattaaa gttgcaagtt atttttttca
    33241 aattttaaga atgtattatt ctaataaaaa atagggggat ggttttgttg aaagaggaaa
    33301 taaaaagaat gatagattta attggggaca gagagttgtt agaaattatt tatttatatc
    33361 taagaaaaaa aactaaaaat aaatgagtac atataaaaaa gctatcaaaa atgagatgat
    33421 agcttttatt ctttagaaaa tacttctaaa attttcaaaa ttgattcgag ttgctcgtct
    33481 gttaatttgc taaattctaa tactgctttc gctagttttt cgttttcgtc tattgcgcaa
    33541 aaagcctccg ctttcacgga gtttctatcc tcagtgttat acatctctcc tgtgccagtt
    33601 cttagccaat tctcatttac cttaaatagt gaaattatta attttaaatc actttccttt
    33661 accttcgtcc ttccactttc taaattgtaa atctcatgct ttgtctttcc taatttttct
    33721 ccaaattcta tttgctttaa cccctctgat gaccttaact ttctgattcg tatatttata
    33781 tcgtctatta tttcgttcat ttacaaaact cctttcataa attgatttta ccttaaaata
    33841 ttatttaagt caacgtaatt atacgtccaa atggtaaaat gaaggcataa taaatttcaa
    33901 tatagtattg acagtactcc tataacgtca tataatgtat ctatagacgt tataggagta
    33961 ctgggaggtg tttttatgaa tagagaagaa atgatttgta aaagtgtgaa attatattta
    34021 agtttaagcg
//
LOCUS       DAMJVM010000036        33458 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.036, whole genome shotgun sequence.
ACCESSION   DAMJVM010000036 DAMJVM010000000
VERSION     DAMJVM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33458
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.036"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            342..1208
                     /locus_tag="PC523_001801"
     CDS             342..1208
                     /locus_tag="PC523_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2475326.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISKIIPIHDILLQSVYGGILSGLGVGIVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSDEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            1450..2259
                     /locus_tag="PC523_001802"
     CDS             1450..2259
                     /locus_tag="PC523_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475327.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVSKEIRSIKSMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYIGKFLEGKMEGKGSYTFFVDEGT
                     AMEYIGEFKEDKKNGKGNFVFSNGNEYIGYFEEDMMNGIGNMLYNSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEILYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            2400..3464
                     /locus_tag="PC523_001803"
     CDS             2400..3464
                     /locus_tag="PC523_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF2475328.1"
                     /translation="MKKLIALVLVILIVLFYSPELLKEGNDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKNQNVQSNNTEGDNLGESDIKNFEKISLEDNLSYVLSSIGKPGRI
                     DTSEYGFDWYVYNQYGKEFAMVGVENDEVVALYSNSINSSENQGIKINQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEKNRVCSYLIIDKSTEDEFK
                     KLYPDDREELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            3492..4211
                     /locus_tag="PC523_001804"
     CDS             3492..4211
                     /locus_tag="PC523_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2475329.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIILISNFSKVSALMTDTSGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKELI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            4234..4818
                     /locus_tag="PC523_001805"
     CDS             4234..4818
                     /locus_tag="PC523_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDF2475330.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGETVVVKRNNEIKIRDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEYFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSISHTNKGVFRGLKNPLNVTRYHSLIIDSNTVPKELEVTATTDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFIIEARERVHM"
     gene            4820..6169
                     /gene="pabB"
                     /locus_tag="PC523_001806"
     CDS             4820..6169
                     /gene="pabB"
                     /locus_tag="PC523_001806"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HDF2475331.1"
                     /translation="MIREINTTLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVINDTNLPFVGGAVGYLSYDLGNYIETLPRT
                     AIDDIKMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIKLEEKIMDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDTHILSNSPERFIRCVNNRIETRPIKGTRPRGKNKE
                     EDLKLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDGKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            6159..6902
                     /locus_tag="PC523_001807"
     CDS             6159..6902
                     /locus_tag="PC523_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV family protein"
                     /protein_id="HDF2475332.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIKVKNGLPIDLNIHVERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVIDKALRITVFDEGYNISIRDIPYNQEMYEKGF
                     RLTISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIEELLE"
     gene            6936..7637
                     /locus_tag="PC523_001808"
     CDS             6936..7637
                     /locus_tag="PC523_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475333.1"
                     /translation="MIGCIYEVYNEEISKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDINYFSKDGIKKLLSNREYFFVKEMKDAKVLF
                     DRENISHTIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            7663..8235
                     /gene="folE"
                     /locus_tag="PC523_001809"
     CDS             7663..8235
                     /gene="folE"
                     /locus_tag="PC523_001809"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDF2475334.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLNEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            8301..9092
                     /gene="folP"
                     /locus_tag="PC523_001810"
     CDS             8301..9092
                     /gene="folP"
                     /locus_tag="PC523_001810"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDF2475335.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKISIEMA
                     ISCGVKKEKIVLDPGIGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            9103..9468
                     /gene="folB"
                     /locus_tag="PC523_001811"
     CDS             9103..9468
                     /gene="folB"
                     /locus_tag="PC523_001811"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDF2475336.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYDVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            9461..9967
                     /gene="folK"
                     /locus_tag="PC523_001812"
     CDS             9461..9967
                     /gene="folK"
                     /locus_tag="PC523_001812"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDF2475337.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERKLHRERIVHWGPRTIDVDILFFN
                     DVVSTDERLIIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEERDEVKEL
                     VGYERKPI"
     gene            9948..10733
                     /gene="aroF"
                     /locus_tag="PC523_001813"
     CDS             9948..10733
                     /gene="aroF"
                     /locus_tag="PC523_001813"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDF2475338.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            10988..12778
                     /locus_tag="PC523_001814"
     CDS             10988..12778
                     /locus_tag="PC523_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDF2475339.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMSIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDIINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGLLIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            12796..13962
                     /gene="rpoD"
                     /locus_tag="PC523_001815"
     CDS             12796..13962
                     /gene="rpoD"
                     /locus_tag="PC523_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDF2475340.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHEDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            14124..14813
                     /locus_tag="PC523_001816"
     CDS             14124..14813
                     /locus_tag="PC523_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2475341.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGRVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFKDFIEKKIKTYSSIVSKLEGKNGEAIDKKRNESE
                     VTIKKLENLIK"
     gene            14827..15924
                     /locus_tag="PC523_001817"
     CDS             14827..15924
                     /locus_tag="PC523_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDF2475342.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSADLKGRLIQKLIKNNVSLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDVTKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSKKIKEKLNMKHIRVVGNLS
                     TNITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEISEIEVIKSSVYLNPFSTI"
     gene            15986..16558
                     /locus_tag="PC523_001818"
     CDS             15986..16558
                     /locus_tag="PC523_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2475343.1"
                     /translation="MKAAVIFHSVCGSTYLLAREYKEALKEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGRELLDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSIRGGHPAYG
                     ILHIAGDNSNIRPSDDVKIGIRDYLERLSI"
     gene            complement(16618..17085)
                     /locus_tag="PC523_001819"
     CDS             complement(16618..17085)
                     /locus_tag="PC523_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2475344.1"
                     /translation="MFKEMRLKKREMTKEDTIEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFDDSIYFHSARNGHKLDNILKNNKVSFLVVANENVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDVNLTTVVKIEIDHISGKASRL"
     gene            complement(17471..17947)
                     /locus_tag="PC523_001820"
     CDS             complement(17471..17947)
                     /locus_tag="PC523_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDF2475345.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLNVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(18064..19155)
                     /locus_tag="PC523_001821"
     CDS             complement(18064..19155)
                     /locus_tag="PC523_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HDF2475346.1"
                     /translation="MSKIECLFTIFPILILMYATFYFMPYFVGKKHIYGVSIDQEYKN
                     YNHFIKLDKKFKNLLSLGFIIDLILVFILVFTFNKLELSYFISIIVFLLYESILYIHT
                     HKKAKNLKSELYSKLGHIDVDSKLIIDMDFINKKNKIIKKFKIIYLIPILFTFGMSIF
                     IVFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTNFCLMVLLYITSIGSIKSR
                     IKIDTNRIKESRIENIEYLNKIGYLFLILMTIMTIQFFTTILSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKSNSSYSPEENEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFLLFSLIKSLLL"
     gene            complement(19148..19537)
                     /locus_tag="PC523_001822"
     CDS             complement(19148..19537)
                     /locus_tag="PC523_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2475347.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDNYEVKYYE"
     gene            19729..20175
                     /locus_tag="PC523_001823"
     CDS             19729..20175
                     /locus_tag="PC523_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475348.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMLTGAVLTGVGAYAVYKMIKNRK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKDDVDLHEDAKKDNIYKEDEYKKYEEE"
     gene            20442..21146
                     /locus_tag="PC523_001824"
     CDS             20442..21146
                     /locus_tag="PC523_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2475349.1"
                     /translation="MVKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSGKEMISSN
                     EYSLILLDVTLPDGTGYELCQYIRGFSQIPIIFLTACDEEVNIVMGLDIGGDDYITKP
                     FRIRELISRIKAVLRRKANTVEESKKILKFGDLSIYTLEARVYKNDKEVFLTSVEYKL
                     LLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCEPIYIETV
                     RGLGYKWIGSENSVSI"
     gene            21124..22377
                     /locus_tag="PC523_001825"
     CDS             21124..22377
                     /locus_tag="PC523_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2475350.1"
                     /translation="MKIVFLYNPEVKKFLSKYVTLIFVIIIISIGFSVINVSLTKDMI
                     VRNNQAIIGTLSSKYPNLESEIVDIITQGKSMENTDYGKKILSKYNYDKSIRINSEPI
                     ISKLVLDTIKINIILVCIIFILIFVLVVRYFKSIYNDLSDMTKYVYYSSEGKSFDMKN
                     KNQEGQIGLLKTELLKMTTILNEKVELLKTEKIFLNNTISDISHQLKTPMTSLIMLND
                     LLYNDIPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHR
                     AMQSMKIPMEIKNQKLTIEGSDNISYIGDIDWSVEALVNIIKNCVEHTPEFGNITITY
                     KENPLFSELIIKDDGEGIHKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNG
                     DIYVNSEKGKGTEFHIIFHKMYDSD"
     gene            22457..23134
                     /locus_tag="PC523_001826"
     CDS             22457..23134
                     /locus_tag="PC523_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2475351.1"
                     /translation="MEVLRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLNDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            23135..25696
                     /locus_tag="PC523_001827"
     CDS             23135..25696
                     /locus_tag="PC523_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2475352.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQVRETIKNDGSFHVTFYDVNRKNVEYVTKSAEVEKYAFTKQLGYSKLENSENGIVS
                     IKQYDKNAINGYKISIKEGRFPAKEGEIVLSENIINLIDDNLKIGDKITLKVGDMFDS
                     SKKKIDSMVFHEGDYIASEKDRTFEVVGIIKKPGFERYNGIATAITYFNPMDNYNDTI
                     NVSVEVRNPKEVYEVSNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYADINSSI
                     KSIIIAVTILVIICTIATVYNSFSISITERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTQMSLRIVYNPIIIIVSIIIVLFTIF
                     ISILLPAISASNISPLNVIKNTGEYKVRKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IVTLFSLMISVIIFISFSGFTLLLLRSEEIRNSQRKYDLYLTAKGTARSIDDTVSELE
                     GIDGIKNFELATGQYVSIRVSENKINKSKEDLIGKYYQKYKVGDSYEYDFSNNDLIFP
                     GDFAIRNKINNLIQGSFNKERAIQENGVILVRKSSFESKGKKGVVELTNYKVGDTVTC
                     EYLDENASSKKIKVKILAITDEERLGMGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESAITESEKQNLKVIKIFVYGFIAVISLVSVTNI
                     LNTVSTSINLRKGELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGMTLIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPISKLNKENIIDNIRRESI"
     gene            25916..27190
                     /locus_tag="PC523_001828"
     CDS             25916..27190
                     /locus_tag="PC523_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2475353.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKVECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHESCVKCGKCIAACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(27352..30393)
                     /locus_tag="PC523_001829"
     CDS             complement(27352..30393)
                     /locus_tag="PC523_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HDF2475354.1"
                     /translation="MFKRKLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNSESYTKTKALAKEKIQTLLSKYGVVNAQYALIDNGKIEISGNGGVYSKQD
                     NKNLTKDNMYSIASISKMFTTTAVMKLVDDGKVNLDTPVVNYIPEFKMADNRYKEITP
                     RMLLNHSSGLMGSSFKNTLLLGDNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTTENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGNSQFDEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSHLKENSGLYASSNMVKVDVNDNG
                     TLTVSSPYIENGPEDKYIYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRNGKGYYLVDEKYTSQSYMFGSVKATPGLSD
                     ETPGYIVNTKIIDENNSNAFIEIPGVIGRDLSDIKLYKENGTEYLSFATQTYVSEDSI
                     TNLPAEKSFTCELASNGYAKWYKIGDDIANKKIEVNLPQNSSFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINENSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVI
                     LVGNSLDYNQKELFKTMRFKSITQIGGNGNENSFKQIKEIA"
     gene            30601..30900
                     /locus_tag="PC523_001830"
     CDS             30601..30900
                     /locus_tag="PC523_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDF2475355.1"
                     /translation="MYKNINKNQLKEMMKNEKDILLLDVRTKDEFREHKIENAINISL
                     QELINNIDEIYEYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMN"
     gene            complement(31136..33364)
                     /locus_tag="PC523_001831"
     CDS             complement(31136..33364)
                     /locus_tag="PC523_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDF2475356.1"
                     /translation="MIHLKKTNSLKEILNWNNIKNLKPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITNDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVINNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSVSALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAESKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIETLNKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIILLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRIIKPFNDKFGDSNLT
                     GH"
BASE COUNT        13218 a         3657 c         5631 g        10952 t
ORIGIN      
        1 tttgctaaaa taaaataaat tactgctaaa atggatattt agaaaaaaat actataaatt
       61 tactatattg caagttaaat aataagtagt atcatactat gctagaaaaa gtttcattta
      121 aagcataaat gagatgattt gaacgttttt aaagattgga atataaaaga ttacactaga
      181 catatatgaa aaagataata taatagatga tgtaatagat gacagttcaa gaaattatat
      241 aaaaaattta tttgaaatat tatctattac atcaggaata atatatgtaa gagtaagctt
      301 ttaaaattct aaggatttag aaagattgaa gggagaaaga aatgagtgaa gttaagaata
      361 ccctacaaaa acatccagtt ttaagatatt caacaatgct atttgggatg ttgatgacat
      421 ctataggaat aaatgggttc ttaagaccag cacatctact tagtggaggt gctacaggta
      481 tagcaacatc tataaactat ttgaccaata taaatgtagg tttattaaca tttttaatca
      541 atattccaat atttatctta gggtttatat acctagaaaa agagttttgt atttcaagtt
      601 tggtaaatat gattgtattt tcattattat taggtgcaac tcaggaaata agtaagatta
      661 tacctataca tgatatattg cttcaaagtg tatatggagg tatattatct ggtttagggg
      721 ttggaattgt atttagaact aggtcttctc aaggtggtac agatataata gctgcaatac
      781 taaaaattaa gaagaatata gaaatgaaag acactgcctt agcaataaat ggcttgatag
      841 tacttacagg aagtttttta tttgggttag atttagcatt atacactctt ataggtctat
      901 ttctaaatgc gtattcaatg agttttatta aagatgcaat gaattaccaa aaatctgtta
      961 tggtaatgtc agatgaagtc gatttaatag cagaagatat aatgaagagt ttagttagag
     1021 gtgtaacttt tttagatgca gaaggtgcat atactcatca aaaaaagaag attatatata
     1081 caatagtttc atcaaatgag ataccaaaga taaaagacat agctttgaag tatgataaaa
     1141 aggcttttat atctgtaaat gatgttacag aagtaaaagg aagagggttt aaagcaaaag
     1201 acttatagaa aattttaaaa gctgggagtt tgtaatgtaa atttgtacat tatatctctc
     1261 agcttattta aatttagtat atgaaaataa aggttttatt attgatttgt gtatcacttt
     1321 aaaacatatc attttaaaat taaaattgaa aaaatcactc aatatgtaga aaacatgacc
     1381 actattagga tataataaat ttaatagatt tagtaaaata tctaggaata acgttggagg
     1441 aatggcatta tgaatttatc taaagaatat gttaatttgt atgataatta tttgaaatat
     1501 ataaatgaag taagcaaaga gattagaagt ataaagagta tgaaaaaacg tgttttaaaa
     1561 aggggagatt ttgctccaga aaatgaagta ttagaattgg aaggaaaaac aatacgtgat
     1621 atagatgatg tagatacaaa aaaaccgaaa aataaaatat ataaattttc aaatggggat
     1681 gtatatatag ggaaattctt agagggaaag atggagggga aaggctcata tacttttttt
     1741 gtagatgaag gaacggctat ggagtacata ggagaattta aagaagacaa aaaaaacgga
     1801 aaaggaaact ttgtattttc aaatggaaat gaatatatag gctattttga ggaagatatg
     1861 atgaatggta ttggaaatat gttatataat agcaaagatg aatatattgg aacttggaaa
     1921 aatggaaaaa aagatggaaa aggtatttat aagtggagtg atggatgtat atatgcaggt
     1981 gaatttaaag gtgggaagat ggaaggatat ggcatctgtt ataatagtaa aggtgaaatc
     2041 ctttatgaag gagaatggaa gaataatttg attcatggta agggaactta tatatgggaa
     2101 aaaggtaaaa aatatatagg tgaatttatg catggtaaaa aacatgggca aggaactttt
     2161 tatctaaata atgaattggt ttatgaaggg acttggaaat ttgataagcc atctatattt
     2221 gatagaacat tagatgaaat attttccttg aggctgtagc tttatctaat tagataaagc
     2281 ttttttctaa tacttactgt aaataggtat gaatgatatt gattagtata cactatcaat
     2341 attagcctaa taaatgtaat attaaaaaat tttgattgat tttaaaaagg agagatatat
     2401 tgaagaaatt aattgcttta gtgttagtaa tacttattgt tttattttat agccctgaat
     2461 tgttaaaaga aggaaatgat ttaaaagaaa agttttttca aagtgaaata tggaatgaat
     2521 ttttagataa aataaatata aatgataatt ctaagaaaga taaaaaagaa aaaaatcaaa
     2581 atgtacaatc aaataatact gaaggggata atttaggaga gtcagacata aagaattttg
     2641 aaaaaataag tttagaagac aatttaagct atgttttatc aagtattggt aaacctggta
     2701 gaattgatac tagtgaatat ggttttgatt ggtatgtata taatcaatat ggaaaagaat
     2761 ttgcaatggt tggagttgaa aatgatgaag tagtagcact ttatagcaat tctattaatt
     2821 catctgaaaa tcaaggtata aaaataaatc aagatagaca gacagtgaga actaagatta
     2881 caccgttaaa gtataagaga aaaggaaata ccagatatat aataaactca gaaaatcaat
     2941 atgatataat aagtaaagaa ggtaagtaca ttacaatttt ctacgatatt catgaaaaaa
     3001 atagagtttg ttcatattta ataattgata aaagtaccga agatgagttt aaaaaattat
     3061 atccagatga tagagaagag cttaaaaaat gttttgaact tgaagttata gaccttgtaa
     3121 atagtgttag aaatcaaaga ggattaaatt cactgagata cagtgaacag gcaactttga
     3181 gttcaagaaa acatagtgaa gatatgagag ataataattt ttttgaccat gtaaacaaga
     3241 agaatgaaac tccatttgac agaatgaaaa gagaagggat agtatatact tcagctggag
     3301 aaaacatagc agcaggacag attaatgcaa tatatgcaca tgaagcttgg atgaactctg
     3361 aaggacatag aaaaaatatt ttggggaatt ataataatat tggagttgga gtaatatttg
     3421 gaggaagcta taaaacatat tacactcaaa atttttataa ataactaaaa tatttgaaag
     3481 gaagtttaaa aatgacatca tggaaaaaaa agacagtata taagtgttta atagcagtag
     3541 tcttgttttg tggaataata ttaatatcta attttagcaa agtttctgct ttgatgacgg
     3601 atactagtgg aaatgttttg attaaacatg gttcaagaga aaaaaagtta atagcaatta
     3661 cctttgatga tggtcctcat cctaaagaaa ctagtcaagt tttagatgtg ttaaagaaat
     3721 acaatgtaaa agctaccttt tttatagctg gaaaacatgc aaaatggtat aaagaaccat
     3781 tagtaagagc aagtaaagaa ggtcacgaga taggaaatca tactttcaat catcctgata
     3841 taagtaattt aagttctagt cagatagaag aggagattgt taagtgtgaa gacattttga
     3901 aagaagtaac tggaaaaaaa cctacattat ttagaccacc atttggaagt tatagagaaa
     3961 aagaattaat agaaattgca aagaagcatg actataaagt tgttttatgg acaggcgtag
     4021 atgtgaaaga ttggaaaaat ccaggagcta atagcattgc agataaaata attaataaag
     4081 tacaaaatgg agatataata cttttacatg attatgcaac taatgataca gttgaagctt
     4141 tagatatgtt tatcccaaaa atgattgaaa aaggcttcaa atttgtaaca gtttctgagt
     4201 taataaaata atttttaggg ggagatttac agtatgattc ttatgataga caattatgat
     4261 tcttttgtat acaacctagt acaatatata gaagaattag gggaaacagt tgtagtaaaa
     4321 agaaataatg aaattaaaat aagggacatt gaagagctaa atccagaggt aattgtgtta
     4381 tctccaggac catgctctcc aaaagaagct ggtatttgta tagatattgt tgagtatttt
     4441 aaggggaaaa aacctatact aggaatttgt ttaggtcacc aaactattgg acatgttttt
     4501 ggaggagata ttataaaagc tcaacagcct gtgcatggaa aagtatatag tattagtcat
     4561 actaataaag gagtttttag ggggcttaaa aaccctttaa atgtaactag gtatcattca
     4621 ctaataattg attccaatac agttccaaaa gaactagaag ttacagccac tactgataaa
     4681 ggggaaataa tgggaattag acataagaaa tatttaatag aaggagtaca gtttcatcca
     4741 gaagctattt tgtcagagta tggtcatgaa atgcttaaaa actttataat agaagctaga
     4801 gaaagggtac atatgtaata tgataagaga gataaatact acactaaatt cttttgagat
     4861 atttactatt tttagaaatg aacatgacag ttttatatta gacagtgcta tggataaaga
     4921 aaaattggga agatattctt ttataagtag ccaaccattc aaggtattaa aatataagga
     4981 tactgatgaa aatcctttag aagttttaaa agaagaacta cataaatata gagttataaa
     5041 tgacactaat cttccatttg taggaggtgc tgtgggatat ttatcctatg atttaggtaa
     5101 ttacatagaa actttaccta gaactgctat agatgatatt aagatgcctg atatgtattt
     5161 tggattttat aatcatgtaa tagttataga ccatcttgtg caaaaaactt atatagctac
     5221 tccaaacata gatataaaat tagaagaaaa gataatggat gatatagaac aaagaatatt
     5281 aaaagaagaa aaaaaaggca tagatagtat atgttatgaa gaaaaagaag taacacctat
     5341 aagacttaaa tctaacttta caaaggaaga atttaaaaat gcagttcaaa gtgttagaga
     5401 gtacataaga caaggagata tatatcaagc taatttaaca caaagattca gtggagaaac
     5461 agaacttaca agctttgaat tgtataggga tctaagaaga tttagcccag ctccatttgg
     5521 agcattctta aattttgaag acacacatat attatcaaat tctccagaaa gatttataag
     5581 atgtgttaat aatagaatag aaactagacc aataaaaggt actcgtccaa gaggtaaaaa
     5641 taaagaagaa gatttaaaac ttcaacaaga acttagaaat agtgaaaaag acagagctga
     5701 actgcttatg atagtagatt tagaaagaaa tgatattgga agaatatcta aaactggaag
     5761 tgttaaagtt ccagaactat ttgtaattga accatatgca aatgtaaatc acttagtttc
     5821 cacagttgta ggtgagttaa aagatggcaa agatgctact gatgtaataa aagcaacttt
     5881 tccagggggg tctataacag gagcacctaa gattagagca atggaaatta tagatgaatt
     5941 agagccaaca caaagaaatg tatatactgg ttcaataggt tatataggtt ttaatggtga
     6001 tatggatttt aatatagcaa ttagaacaat aatcaaaaat gacaaaaaag tctattttca
     6061 agttggagga ggtatgactt gggattctga cccagatgaa gaatatcaag aaacacttga
     6121 taaagcaaaa tctataatga aggctttgag gggatattat gaagaataat gtaggttttg
     6181 atagtagttt aagtaaattt ggaattggtt tgtttgaaac tattaaagtt aaaaatggat
     6241 taccaataga tttaaatatc catgtagaaa gaatgctaag ttcaataaat tgtttggatt
     6301 tagatataaa ttataaaaaa gatttcttga taaatgaaat tgttacatat ataaaaaaag
     6361 aaaatgttat tgataaagca cttagaatta ccgtttttga tgaaggttat aatatatcta
     6421 ttagggatat tccatataat caagagatgt atgaaaaggg gtttagatta actatatcac
     6481 cgatagttag ggggaatagt ttaattcata gacataagac tactaactat tttgaaaata
     6541 tatataccaa aaatatcgca aataagactg gttataatga tggtatattt ttaaactcag
     6601 aaggtgtaat attagaatgt tctatgagca atatcttctt tataaaagga gaaagagtgt
     6661 acactcctag tgatagattg ccaatattaa atggaataat aaaaaaaagg attatagaga
     6721 tttgtgacga attacatata gagttgatag aaaatgaaat taatatatca gaaattagta
     6781 gttttgattt tgtttttgtc acaaatagtc ttatgggcgc tataaaggtt acagaaattg
     6841 acaaaataaa atttaataaa gaaaatgttg tttttgataa aatagaggag ttattagaat
     6901 aaattaaaat attaacaatt tgttggagac aaaagatgat aggctgtatt tatgaagtat
     6961 acaatgaaga gataagtaaa ataatagaaa accaaaatac tttggctgta tatttggtgg
     7021 gttctagtaa agatgtagat tttactcttt atgatgtaga aataaacgat attgatgtat
     7081 tcgtttttgt aaaagaaggg gaaaaacaag agagaatact atttaaaaaa aagggaatag
     7141 aatttgatat aaattacttt tcaaaagatg gtataaaaaa attattatca aatagggaat
     7201 acttttttgt gaaagaaatg aaagatgcta aagtactgtt tgatagggaa aatatatcac
     7261 atactataaa agatataagt agagatattt acttagaagg acctagtaaa atgtcattag
     7321 aggaaaagtc ttttataaag caagatattg gagcaaaaat atcgaattta aaaaataaag
     7381 aaaaatttga tgtttttgaa tatcattttt tgacgaattt atatttaaaa gatataataa
     7441 ttgggtactt taatataaat gataaatggg taccaaaaga taaaaaattg ctaaaagttt
     7501 taaaaaaaga aaataatgag ctttttgaac tagtgtcaaa agtaagtgaa aattatgact
     7561 atcaaagact tttagatgtt tataattata tttttaaaga aattgagaca aaagaggtta
     7621 taaaactaat cttctagtca caaatcaatg gggggagttt aaatgaataa agttgataaa
     7681 gaaaagatac aacatgcagt aagagaaata ttagaggcaa ttggggaaga cccagataga
     7741 gaaggtctaa tcgagactcc aaatagagtt gctaggatgt atgaagagat attctctggt
     7801 cttaatgaag aaccaagaga ccatttaaaa gttctttttg cagatgaaaa acatgaagaa
     7861 ttagtgttgg taaaagatat tccattttat tcgtgctgtg agcatcatct agttcctttc
     7921 tttggaaagg ctcatattgc ttacttacca aaaggaggaa gacttacagg tctttctaaa
     7981 cttgctagag taatagatac attggcaaaa agaccgcaat tacaagaacg aatcactaaa
     8041 aatgctgcag atataataat ggaagaatta caaccttatg gtgtcttagt agtagtagaa
     8101 gctgaacata tgtgcatgac aatgagaggt gtaaaaaaac caggttcaaa aactgttaca
     8161 tcagcagtga gaggtatatt tgaaaaggac attgcgtcta gagctgaagc tatgagtttg
     8221 attactatga agtagcaatt tattatatat agaatcattt attgagatat gaaatttaat
     8281 ttaagggagt gtcattaatt gtgtttgatt atggtaaaag aacttatata atgggaatat
     8341 taaatgtaac tccagatagt ttttctgatg gaggagattt taataactta gacatagcaa
     8401 tacaacatgc aaaagacatg gttgaccaag gtgcagatat aatagactta ggaggagagt
     8461 ctacacgtcc aggtcatagc tatgtggatt cagatgaaga gttaagaaga gtaatccctg
     8521 taataaaaaa acttaaacag gaattggata taccaatatc aatagacact tataaggcag
     8581 atgttgcaga agaagcctta aaactaggtg ttactatggt aaatgatgtt tggggtctta
     8641 gaaaagataa aaatatggct tctgtaatag gaaaatatga tgcagaagtg tgtataatgc
     8701 ataatcaaga tgggacaaac tatgacaaag atataatgga gtcaataaaa gattttttta
     8761 agataagtat agaaatggca ataagttgtg gtgtaaaaaa agaaaaaata gtacttgacc
     8821 caggtatagg atttggcaag gattttgaac aaaatattga agtattaaga agattaaatg
     8881 aattaaaaga tttagggtat cctattttgc ttgggacatc tagaaaatca gtaataggaa
     8941 aagtacttcc agttgagcct aaaaaaaggt tagaaggtac aatagctact actgttcttg
     9001 gtataagaga tggtgtagat atagttagag tacatgacgt ttatgaaaat ttaatggcag
     9061 ctagaatgac tgatgcaata tatagaaagt aggtataata taatggataa aatattactt
     9121 agtaatttag gattctatgg atatcatggt gttcttaaag aagaaaattt tctaggtcag
     9181 aaattctttg ttgacatgga actttatatt gattcaagag aagcaggtct tagtgacgat
     9241 ataaataaat ctgtaagcta tgctgaggtt tacgatgttg ttaaagatat tactgaaaat
     9301 aaacaattta atcttttgga ggctttggca gaaaatattg ctgaagaagt cttgaataaa
     9361 tttatactta taaatggagt tatggtcaga gttagaaagc ctgaagctcc tgtaaatggt
     9421 atttatgact attttggtgt tgagataagg agaacaagag atgaataagg cttatttggg
     9481 aataggtact aacatgggtg atagatttga taatttatca agagcatgtg aacttttgaa
     9541 aaacagcgat tctatatata aggtaaaaga atcaagttta tacgaaacaa aaccatgggg
     9601 ttatacagaa caagcagatt ttctaaatat gtgtgtagaa atagaaacag agtttgagcc
     9661 ttatgagttg ttagaatatt gtcaagaaat agagagaaaa ctacacagag aaagaatagt
     9721 tcattgggga ccaagaacta tagatgtaga tatactattt tttaatgatg tagtttccac
     9781 tgatgaaaga ctaataatac ctcacccaag gattcaagat agagctttcg ttttgatacc
     9841 acttatggat ttaaatgaag aacttataat aaatgaaaaa acaataaaag aacatttaaa
     9901 cctcctatct gctgaagaaa gagatgaagt aaaggagctt gtaggttatg aaagaaaacc
     9961 tatttaataa taattcaaag atagagataa acacaaataa caaaaagata actgtaaaaa
    10021 aggataaact tataatagct ggaccatgtg caatagagag ttatgagcaa cttttggaga
    10081 cggctaaatt tgtaaaaagt cagggagcaa atattttaag aggtggagcg tacaagccaa
    10141 gaacatcacc aaattcattt caaggattaa aaaaagaagg tcttgaaata ttaaaagcgg
    10201 ttaaagatga agttggtatg gctgttatta cagaacttat ggatgtaaga gatatggacg
    10261 aattgtatag tataagtgat ataattcaaa ttgggtcaag aaatatgcaa aactttactc
    10321 ttttaagtga agtgggaaaa caaaataaac ctgttatgtt aaaaagagga atagcatcca
    10381 caattactga atggataggt gcttctgaat atatagcaat agaaggaaat agtaatatta
    10441 taatgtgtga gagaggtata agaacatata atgattatac tagaaataca ttggatttag
    10501 ctgcagtacc cattattcaa aaggaaacag gtcttcctgt tgtggtagac ccaagtcatg
    10561 ctactggagt taggtatttg gtaaaaccta tgtcactggc atcttttgca tgtggagcag
    10621 atggaataat ggtagaagtg catcctgacc cagaaaatgc tttgtcagat ggtgctcaat
    10681 ctctttgttt taatgaattt gaagatttaa tgaaatcaat taataattat taaaagaggc
    10741 atgagcctct tttttattcg taatcgttat taaggtaaat ttataagtta taatttgatg
    10801 gttatattat ttattataaa taaagattta atattgatag aattaatata aaaaataaaa
    10861 attttttgat taataagaag gatatcaata aaaaaagtcg aattgtgtat attgagtgat
    10921 tgacaaattt agaatatagt gtagaataga aatgttaaca caaaaaatta caccagaagg
    10981 tgataatatg aatgatataa aagacattat tgaagaaata aaagctagat gcgatatagc
    11041 aagtataata tctgaatata tgagtataaa acaatctgga gctaattaca aaggattatg
    11101 cccatttcat ggcgaaaaga caccatcttt ttatataaat acatcaaaac agatttataa
    11161 gtgtttcggt tgtggtgaag gtggagatat aataaatttt gtcatgaaaa tggaaaattt
    11221 agattttatg gatgctgtaa aaattttagc aaataaatgc ggtatagaaa ttaacactaa
    11281 catgaatgaa gaaactagaa ttaaaataga aaaatctaag aaatttcaag atattcacac
    11341 agaagcagct agattttatc tttcaaactt attgggaagc aaaaatcttg ggtatgaata
    11401 tttaagaata agaggactag atgataaaat tattaagaag tttggtttag gattttcttt
    11461 agattcatgg aattcactta tgaatactct aatcagtaaa ggatataaaa aacaagactt
    11521 attggagtgc ggtctaattg caaaaaatag agatggaact aattgctatg ataaatttag
    11581 gaatagagtt atgtttccta tattcgatta tagaggaaac ataatagggt ttgggggaag
    11641 agtattagac gattctttgc caaaatactt aaattctcca gacacattaa tttttaataa
    11701 aaaacagaac ttatacggat taaattttgc tagaaaaaat ttagaaagta aaactatagt
    11761 cttagttgaa ggatatatgg atttaatttc gctttatcag tatggtataa aaaatgtagt
    11821 agcaaccctt ggaacagcct tgactgaaca acaaggcctt ttaataaaga gatatgcgga
    11881 cactgctatt atatcttatg attctgatga agcaggaata aaagcgactt taagagctat
    11941 agacatactt actaaattag gtatcaatgt aaaagtgttg gacttaaaag atgctaaaga
    12001 cccagatgaa tttgtaagaa aatacggact tagtgattat aaaaaagcta tggatgtttc
    12061 tactcactac ataaaatata aaatagatca tcttaaaaaa gaatttaata ttcaaaaaga
    12121 tgaagaacga gtgaagttcg caaaagaagc ttctaaaatt ataaaacaat tgacaagtcc
    12181 tgttgaaatt gatttttata caaagtattt aagcaatcaa atagatatta atgtcgagtc
    12241 tataaagaga gaagtctatg gtaaaaatta caataagcca tataataaca aaaatcaaaa
    12301 gaaaatagaa gaaaaagtaa ttgaaaaagt cgaagtcaga caagacggaa aacaattagt
    12361 tgaagaaact ctaataaaaa taatgttaga agacaaaaaa attagagaaa tagcgttact
    12421 taaagtagaa gaaagtgatt ttttattgaa agaaagtaaa gaaattttaa attacatgat
    12481 taaaaatcaa gaattggaca aaataactat tgacaaattg aaaagtttaa acatatccga
    12541 agaatactta aaagaattaa attcaatttc tttgaatagt ataaacttag agaatacaaa
    12601 agaaatagaa ggaataataa caaatatcag aaaaaactca ttagaagagc aaattaatag
    12661 cttgttgaga gaacaacaag aattagaaaa taataatgat atgaaagagg tagatggtag
    12721 agttatggaa attgctttaa aaatagttga gataaataaa attttaaaga gcttgtagag
    12781 aaaggaggtt tgagtgtgga aaataaatcg aataaaaaag agttaaaaaa ggttactgct
    12841 aagacattaa tagaaaaagg aaaaaagcaa ggttctctga cacttgcaga gataatggaa
    12901 gctttttcgg agactgaact tgataaggat caagtagaaa atctttatga gactttggga
    12961 aatttgggaa tagaaataac agaaacaaaa aattataaag ctgatataga tttttcggtt
    13021 gctgatgacg atttaagtat aggccactta gatgaagatg cagaagcaat ttcacatgag
    13081 gactcttctg caatagaaat agaaactgtg gatttatctt taccaaaagg gataagtata
    13141 gatgaccctg taagaatgta cttaaaagaa ataggaaaaa ttcctctact taaaccacat
    13201 gaagaagtag aatttgctag aagaatgcac gaaggtgatg agatagcaaa acaaaggtta
    13261 gttgaagcaa acttaaggct agttgtaagt atagcaaaaa gatatgtagg aagaggtatg
    13321 cttttcttag atttaataca agagggaaat ttaggtctta taaaagcagt cgaaaaattt
    13381 gactacacaa aaggatataa gtttagtact tacgcaacat ggtggataag acaagctata
    13441 acacgtgcta ttgcagacca agctagaact ataagaatac cagttcatat ggtagagact
    13501 ataaataagc taataagagt atcaagacaa ctacttcaag aacttggaag agatccaaag
    13561 ccagaagaaa ttgcaaaaga aatggaaatg acagaagaca aagtaagaga aataatgaaa
    13621 atagctcaag accctgtgtc tttagaaaca ccaataggag aagaagaaga tagtcattta
    13681 ggtgatttta ttccagatga tgatgctcca gccccagcag aggcagcagc atattcttta
    13741 ttaaaagaac aaatagaaga tgtacttggt tcattaaatg atagagagca aaaagtatta
    13801 aagcttagat ttggacttga ggatggtaga gctagaactc ttgaggaagt tggaaaagag
    13861 tttgatgtaa ctagagagag aataagacaa atagaagcaa aagcattaag aaaactaaga
    13921 catccaagta gatcaaaaaa acttagagat tatttagact aaaaagatac atcattaatg
    13981 tttttgtgat aaatgccatg agataagtga tatctcatgg ctattttgta gtaaagattt
    14041 aattttggtt ttgtagttat ttcagtctta tctataactt agttaaatta tatggataaa
    14101 gtgaattgaa aggagaatta tatttgaaat taacagatag attattgaaa atagcatcat
    14161 tggtgtcaga tggaaaaaaa atagctgata taggaacaga ccatggatac ataccagtat
    14221 atttgttaaa agagggaaga gtaccatttg cagtgttggc agacgttaat aaaggacctc
    14281 ttgacaatgc ccataaagaa gtcatacaga acaatctttt agataaagtt gatttacgat
    14341 tgggttctgg catagagatt cttgaaatag gagaagtaga agaagtaata attgctggta
    14401 tgggtggaat tttgataagt gagcttctcg aagctaaaaa agaagtagca cataatgtag
    14461 aaaaattaat attacagcca atgcaagcgc aagaagaact tagatattac cttttaaaca
    14521 atggttatga aattttagaa gaagttttag tgagagaaga ttttagaata tatgaaataa
    14581 ttgttgccaa atatacaggt aaaaatacta taatagaaga tgagatatat tatgaggttg
    14641 gaataaagct tttagaaaac aaggattctc tttttaagga ttttatagaa aaaaagataa
    14701 agacatatag ttctatagtt agcaaattag aagggaaaaa tggggaagct atagataaga
    14761 agagaaatga aagtgaagtt acgataaaaa aacttgaaaa tttaataaaa taaataggag
    14821 gaatatatgc tattaaagag tctaactaga aaaattgaaa aaaaataccc tctaaactta
    14881 gctgaggatt gggataatgt aggtcttata gttggagatt ttgatatgga tgtaaaaaag
    14941 gtattggtat ctttagaagc taatgaagat gttattgatg aagctatatc taaaaatata
    15001 gatttaattg tgacacatca tccttttata tttgggaaaa ttaataaaat taatagtgca
    15061 gatttaaaag gtaggcttat ccaaaaactt attaaaaata atgtatcact ttattctatg
    15121 catactaatt ttgatatagc atttgatggt ttaaatgact attttatgga aattatggaa
    15181 tttggtaatt caaaggtttt agacgtaact aaaagcgaaa acttatataa attagcagta
    15241 tacgtgccac ataactattc agatgaactt agaaaggttt taagtaattc aggagcaggt
    15301 catataggaa attatagtga ctgtacgttt tcaatagaag gtgaaggaca atttaagcca
    15361 ttagaaggct ctaatccgtt tcttgggagt gtaaatgata tagaaactgt aaatgaagtt
    15421 aaaattgaaa ctgtagtacc acagaagctc ttaggtgggg taataagttc tatgttagat
    15481 gcacatcctt atgaagaagt tgcatatgat ttgtacaagt tagaaaataa aggtgagata
    15541 tttggactag gtagaataag taaacttgat aaaagtatga cattagaaag tctttcaaag
    15601 aaaataaaag agaaattaaa tatgaaacac atcagagtag ttggaaatct tagtacaaac
    15661 ataacgaaag tagctgttgt aacaggagct ggttcagagt ttgtaaaaaa agctaagaga
    15721 caaggtgcag aagtactaat tacaggtgat gtaaagtatc atgaggcaca agatgcactt
    15781 gatattggta tgtgtatagt ggattgtggt cattttgata cagaagatat atttaaaaat
    15841 gttatgaaga gatttttaga tgaaattagt gaaattgaag taataaaaag tagtgtatat
    15901 ttgaatccat ttagtactat ttagtagtac ttgattaaaa aatagtatat aaaaaataaa
    15961 tttgagttta attaggggga taaggatgaa agcagcagta atttttcata gtgtttgtgg
    16021 gagtacatac ttattagcta gagaatataa agaagcgctt aaagaaatga atattgaggt
    16081 agatatcttt agagtaagtg acgaagttgc aaagacactt cctcaatatt atttaataaa
    16141 ttctaaagaa tacaaagatg aatttgaaag tataaatgta attaaatctg gaagagagct
    16201 attagattat gatgctatat ttatgggttc accaacatat tacggaaatg tatctggacc
    16261 aatgaagatg tttatggata gtttttctga tatttgggta ggtgctcctt tgagtggtaa
    16321 aatatttggt tgttttgcta cagcaggttc acaacatgga ggaggagaac tagccttgca
    16381 agctatgaat atatttgctc aacatatggg gatgactctt ttatcagttc catgttcaat
    16441 aagaggaggt catccagcgt atgggatact tcatatagca ggagataact ctaatataag
    16501 accaagtgat gatgttaaaa taggtataag agattaccta gaaagactta gtatataaga
    16561 atactatata aaaataccta aaagtggctg tatatacttt taggtatttt tacttaatta
    16621 taaacgtgaa gctttaccag aaatatggtc aatttctatt ttaacaacag ttgttaagtt
    16681 cacatctttc tctatgtact tcatgccttc ttcaaagaat cctttagaat atttttttat
    16741 tatttctaca agagcatttt tcttttcttc attttctaca gtacaagctt tcccaaaaac
    16801 tatagcacta gaataagtag tactaaattt gtctggaata acattttcat tagcaaccac
    16861 taaaaaagaa actttgttgt ttttcaatat attatctaac ttatgaccat ttcttgcaga
    16921 gtgaaagtag atagaatcat caaagtatac ataatttaca gcaactccat aaggataacc
    16981 attttcagat atagtggaaa aagtaccaaa ttcaccattt tttaatactt cgatagtatc
    17041 ttcttttgtc atttctcttt tcttcaatct catttcttta aacataatat catcctttct
    17101 aattttgatg cttaaattaa attttagagc atatataaat attatgctat gttttttata
    17161 aattttaata agaaaatata gtttaagata aaaggttgag aaagagaatc tatttggggg
    17221 ttcatttatg taattattgt tagagtatta ccaacaatat taataacttt aattaatgac
    17281 ttgctgttat ttaaggtagc aagcttgtct ataagaaggg aaagttatcg aggatagaag
    17341 ttatagttta taatgatatt ataaagaaaa tgttgttgta gaaaataata aatagtagag
    17401 aggtattaag ttgaatataa tacttaatac ctctttaaaa ttaaattata aaagtgttat
    17461 ggaaactgta ctatacatgg taaggagata ataaagaatg tatctttaaa gtctcaacca
    17521 aacagcaaaa cttatccaac aaacatacaa taaatgcttc tttgtatcta ggaggcttga
    17581 cagtaagtgg ccacatgtgc tttaagatta tgtccgtttc tttttcattt atttgaaaaa
    17641 ataaagttgc atttttcaat gcttctctag ggtgagtaaa tccatgtaat ccttttctat
    17701 cacctttgta atgccaatca taaagaaaaa agtcgtgtaa taaagcccct ctaataacac
    17761 tttgaatatc aacatttaga tgcaattttt tacataatag ataactataa tatgctactg
    17821 ataaactatg ttcaagacat gaaacattgc catgatgagg aaatgttctc ataagttgta
    17881 ctttttctga agaaagtatt tcagaagcac attcattaaa atatttgatt tcagcttttg
    17941 acaacattat acaaatctcc atttctagta atataaatac tatatttcaa cgatatatga
    18001 aatatttctt atcctacaaa atataatacc caataataca agtaagatac cttttatggt
    18061 attttaaagt aagagagatt ttataaggct aaacaataaa aaaattaaaa gtaaagctat
    18121 agcaatatat agtatttttc caagaggatt gccaaaattt atagtccatc caataccaaa
    18181 ccttttatta accatcaatg atgggtcatt tggattatta tacatgattc ccgctatcca
    18241 atatttttca ttctcttctg gagaataaga agagttcgat tttaaatttg gagaattaat
    18301 gtatgttgct attaaataaa ttataactaa aagcgttgta atattcatag cagttgatag
    18361 ttttgtttta attgataaaa tagtagtaaa aaactgtatt gtcattatag tcatcaatat
    18421 taaaaacaaa taaccaatct tatttaaata ttcaatgttt tcaattctac tttctttaat
    18481 tctatttgta tctatcttaa tccttgactt tatggagcca atagaagtta tatataataa
    18541 aaccattaaa caaaagttag taccaattaa tttaaataca ttaagaaatg atttatctat
    18601 aaaaacatca ggagacccat ttatgttcca atgagtggca atggagtctg gcaattgatt
    18661 ataattaaat actataaaaa tactcattcc aaaagtaaat agtattggta ttaaatagat
    18721 tattttaaat ttctttatta ttttattttt tttatttata aaatccatat ctattattaa
    18781 ttttgaatct acatctatat gacctaattt agaataaagt tctgatttaa gattcttagc
    18841 ttttttatgt gtatgaatat atagtatact ttcatataat aaaaaaacta tgatactaat
    18901 aaaataggaa agttccaatt tgttaaaagt aaaaaccaga ataaaaacta atataaggtc
    18961 tataataaaa cctaaagaca acaggttttt aaatttttta tctagcttta taaagtgatt
    19021 ataattttta tactcttggt ctatagaaac cccataaatg tgttttttac ctacaaaata
    19081 gggcataaaa taaaatgtag catacatcaa aattagaatc ggaaatattg taaataggca
    19141 ttcaatctta ctcataatat ttcacctcat aattgtcaaa taaacttgaa cttaacgata
    19201 aaaattcctc tttactaata cctgataaat aagattttgc cactaatagt tccaagtcaa
    19261 gcttattttt ttgacgagtt gtgttgtcta ttttcattgg taattgagcc acaatagcac
    19321 cttttcttct atcaatattt aagaatccct cttcttttaa ttcgttgtag gatttgttaa
    19381 ctgtatgtaa atttacacca atattttcag ccatacttct tacagatgga agtgattcat
    19441 ttattttcaa ttctcctgaa gctatagctt tagttatttc ttctttaatt tgtacataaa
    19501 tagatttatc gctattaaaa tctaaattta aaaccataaa aaacctccat tatgctttat
    19561 aaatgaaaac gattgttata tacatagtat aacataaaaa ataataacaa acacaaaatt
    19621 taaatttata ttttgtaaaa attgtaattt aaaaaataac ataattttta ttaaaataat
    19681 acagatacta tttatagata gaaataaaaa ttagaaagga gaaaaaatat gttaacatat
    19741 ttaatgaaaa gacaaatgca taaaaaagat gatggaatga gtaaaccaat gatgcttaca
    19801 ggagctgtac ttacaggtgt aggagcatat gctgtataca aaatgataaa aaatcgtaaa
    19861 tctaattctc aaatggtcaa tacaaattat tatggaaata gcgattatga tgattataaa
    19921 tatgacgaat ttgattatga ctgtgactgt agagatacaa aacaggatta tgaatatgaa
    19981 gaattaaaaa aaaaagtaaa agaatttaat tcaagaagaa taaattgtga aaattgtcca
    20041 catgtttctc aagaagaaat gatgcaatat gtaaatagtg ttaaagatgt aaaggatgat
    20101 gttgatttac atgaagatgc taaaaaagat aatatttaca aagaagatga atacaaaaaa
    20161 tatgaagaag aataagtaat attctgtata atcaaacaaa tagtaatatt aaactattta
    20221 atatctagtt tctaatatgt aagttgtaac ttagcttgaa agaatcggta tcattcttta
    20281 gagagttaag taacaactta cattttgttt ttataaattc gaatggctct ttgtgactat
    20341 tttgtaattt tagatatatt ttagtatcaa gggtaattgt gtataataaa acttgtatat
    20401 gcagatagtt taaaattaaa atgaaaagaa aggaaatagt tatggtaaag ttgttaatag
    20461 ttgaagatga tagtactatc tcttttggaa taaagtatgc tttagaacaa gaaggattta
    20521 gtatagatat atcaaaagat ttatccagtg gaaaagaaat gatttcttct aatgaatata
    20581 gcttgatatt gttggatgtt acattaccag atggaactgg ttacgagctt tgtcaatata
    20641 taagaggatt ttctcagatt cctataatat tcctgactgc ttgtgatgaa gaagtaaata
    20701 ttgtaatggg tcttgatata ggcggagacg attatataac aaagcccttt agaattagag
    20761 aattaatatc tagaataaaa gctgttttaa gaagaaaagc caatactgta gaagaaagta
    20821 agaaaatact taagtttgga gatttaagta tatatacatt ggaagctaga gtatataaaa
    20881 acgataaaga agtatttctg acatcagttg aatataaact tcttttaatt cttatacaga
    20941 ataaaaatac tgtgttaagt agaactcaaa tattggaaaa actttgggat gtaacttatg
    21001 attttgtaaa tgacaatact ctaacagttt atataaaaag gcttagagaa aaaataggtg
    21061 atgatttatg tgaaccaatc tacatagaaa ctgtaagggg tcttggttat aaatggatag
    21121 gaagtgaaaa tagtgtttct atataatccg gaagtgaaga agtttttaag taagtatgtt
    21181 actttgatat ttgttattat tatcatatct attggattta gtgtaatcaa tgtatcttta
    21241 actaaagata tgattgtaag aaataatcag gctataatag gaactctatc aagtaagtat
    21301 ccaaacttag aaagtgagat agtagatata ataactcaag gaaagtctat ggaaaataca
    21361 gattatggta agaaaatatt aagtaagtat aattatgata aatctattag gataaactct
    21421 gagcctatta tttctaaatt agttttagat actatcaaaa taaatataat attagtatgt
    21481 ataatattta ttttgatatt tgtgttggtt gttagatatt ttaagtcaat ttacaatgat
    21541 ttatcagata tgactaaata tgtttactat agttcagagg gcaagtcatt tgatatgaaa
    21601 aataagaatc aagaagggca gataggtctt ttaaaaactg aactattaaa gatgacaaca
    21661 attcttaatg aaaaggttga actgttaaaa acagaaaaga tatttttaaa caatacaatt
    21721 tccgatatat cacatcaatt aaaaactcca atgacttctt taataatgtt gaatgattta
    21781 ttatataatg atataccata tgaagtaaaa atcgattttt tgaataagat aaaaaatcaa
    21841 ttaaatagaa tggattggtt gattaaaagc atgcttaaat tatcaaaggt agaagcaaaa
    21901 gtgataaatt ttaaaaagga taaggtgaaa tttagtgaac tcatacatag agcaatgcaa
    21961 tctatgaaaa tacctatgga gattaaaaat caaaaactga ctatagaggg aagcgataat
    22021 atatcttata ttggtgatat tgattggtct gtggaagcat tagtgaatat aatcaaaaac
    22081 tgtgtagaac atactcctga gtttggaaat ataactataa cctataaaga aaacccatta
    22141 ttttctgaac taataataaa agatgatggt gaaggtatac ataaaaaaga tataccacat
    22201 gtatttaaac gattctatag aggaagaagt agttcaaaag aagatagtgt aggaataggt
    22261 cttgcaatgt caaaatctat aatagaaagc caaaatggcg atatatatgt aaacagcgaa
    22321 aaaggtaagg gtacagaatt tcatataata tttcataaaa tgtatgatag tgactaatct
    22381 gtaatattta attcacttta tagtaatagc caaagcctaa tatatgaagt atagaatata
    22441 aatattggag gtagttatgg aagttttaag agtagaaaat ctaactaaaa gttatggtaa
    22501 aaatgaaaca aaagttgatg ctataaaaaa tgtaagttta tcagttgaaa aaggaacatt
    22561 tatagcaata acaggtccaa gtggaagtgg taaaagtacc ttattacatt tactgggagg
    22621 agttgataga ccaactagtg gtaaggttta tataaatgat gttgatatat ataatttaaa
    22681 aactaaggac ttggcaatat ttagaagaag aaatataggt cttatatatc aattttacaa
    22741 cttaattcca gtcttaaatg ttaaggaaaa tatattgctt ccagcagagc ttgacaatag
    22801 agatattgat agagaatatt ttgatgattt aatgaaaaca ttaggtctaa atgataggga
    22861 aaagcacctt ccaaaccaat tatcaggagg tcagcaacag agaacctcta tagggcgtgc
    22921 attgattaat agaccttcta taatattagc agatgagcca acaggtaatt tagatagtaa
    22981 aaattcaaaa gaaatactag aattgttaaa attatcagta aagaaataca atcagacact
    23041 aataatgata actcatgaca aaaatatggc acttcaagca gatagaatta taagcataga
    23101 agatggaatc ataaaaagtg atgaggtgat gtagatgaat ttgtatacat cactaactat
    23161 tagataccta aaacaaaata aaagaagaac cattgtaact ataatcggga taatactttc
    23221 gacagcttta atttgtggta taggaaacat atttgaaagt tttatggatt atcaagtgag
    23281 agaaactata aagaatgatg gaagtttcca tgttacattt tatgatgtaa atagaaagaa
    23341 tgtggagtat gtaacgaaat cagctgaagt agaaaaatat gcatttacca aacagcttgg
    23401 atattctaaa ttagaaaata gtgaaaatgg tatagtaagt ataaagcaat atgataaaaa
    23461 tgcaataaac ggatataaaa tttctataaa agaaggtaga tttccagcta aggaaggaga
    23521 aattgttcta agtgaaaata ttattaatct tatagatgat aacttaaaaa taggggataa
    23581 gataacttta aaagtaggag atatgtttga ttcaagtaag aagaagattg atagtatggt
    23641 atttcatgag ggggattata tagcaagtga aaaggataga acttttgaag ttgttggtat
    23701 aattaaaaaa cctggatttg aacgttataa tggaattgca actgctataa cttattttaa
    23761 tcctatggat aattacaatg atacaattaa tgtatccgta gaagtaagaa atcctaaaga
    23821 ggtgtatgaa gtaagtaata aaataagcaa aaatatatat tcaaataaag ataaagaagc
    23881 agtttctgat atgataaaat acaatgaaca tttattgagg ttacaaggtg ctagtaagta
    23941 tgcagatata aatagctcta taaaatctat cattatagca gtaactattt tagtaatcat
    24001 atgtacaatt gctacagtgt ataattcctt tagtatttct ataactgaac gaaaaaaaca
    24061 gtttgggata ttaaattcca taggtgctac atctagtcaa attaaaagat tagtttttat
    24121 agaaggtatt ataattagtt taattggtat accaatagga ctaatatctg gaacagtagc
    24181 catagactta ttatttaaaa ttataaataa gtattttact gaatcggttg ttactcagat
    24241 gagtttacgg atagtgtata atccaataat aattattgtt agtataataa tagttttgtt
    24301 tacaatcttt atatcaattt tattacctgc aatatcagct tctaatattt cgccacttaa
    24361 tgtgattaaa aatactggag agtataaagt gagaaaaatc aaaagctcaa gacttattaa
    24421 aatgattttt aaaactgaag gagtacttgc atataaaaat attaggagaa atagaaaaaa
    24481 atttatagta actctttttt ctttaatgat aagtgtaata atatttatat cttttagtgg
    24541 atttacatta cttttactaa gaagtgaaga aattagaaat tctcaaagaa aatatgattt
    24601 atatttaact gcaaagggaa cagctaggtc tatagatgat actgtaagcg aactagaagg
    24661 tattgatggg ataaaaaact ttgaattggc aacaggacaa tatgtttcta taagagtaag
    24721 tgaaaataaa ataaataaat ccaaggaaga tttaattggg aaatattatc aaaaatataa
    24781 ggttggagat agttatgaat atgatttcag taataatgat ttaatttttc caggagattt
    24841 tgcaataaga aataaaataa ataacttaat acagggttct tttaataagg agagagcaat
    24901 tcaagagaat ggtgttatat tagttagaaa aagctcattt gaatcaaagg gtaagaaagg
    24961 tgtagtagaa cttaccaact acaaagtagg agatacagta acttgtgaat atcttgatga
    25021 aaatgcctca agtaaaaaaa taaaggttaa gattcttgct ataactgatg aagaacgttt
    25081 aggtatgggt tatcaaaata tgggattaca atttataact tatgatgaag ttgctaaaaa
    25141 tttaaaccta aagttaagta aaagtcttat atttattgat tcaggaggaa gcattaagac
    25201 taagaaggaa atagaagctt tggctaataa aaataatttt aacttctatg atgaaagtgc
    25261 aattactgaa agcgaaaaac aaaatctaaa agttattaaa atatttgtat atggctttat
    25321 agcagtaata tctttagtaa gtgttacaaa tattctaaat acagtaagca caagtataaa
    25381 tctaagaaaa ggagaacttg cagtaataca atctataggg gttactccta aaggttttag
    25441 aaaaatgata tatttagaaa gttttatata tggtatactg tcattgttgt ttgggatacc
    25501 gattagtatt ggtatgactc taattatgaa taaacttatt tcaggtgtaa ttgagttttc
    25561 tccgattata ccatggacag caatagttat atgtatagta agtgttttta taataacttt
    25621 tatagcaggt tatataccaa taagtaaatt gaataaagaa aatataatag ataatataag
    25681 aagagaaagt atatagatat aaagataatt tttaactata taaaatatta ttaaaatcat
    25741 ttaaatgata taaaatatta ttatatcact taaatgattt tttatttata taagaaatct
    25801 aataataaat tactagaggt atttaactat ttagaaagaa ttggtattat ataaagtaag
    25861 aggatatata ataagaaaga atatttgatt ttattggaaa acaaagaaaa agaaggtgat
    25921 tactatttct catataactg cgaagaatat gtataaaagt ttagaagaaa gaataaacaa
    25981 gtttccacaa ggtgctccac cctcagatac attatacaag atactaaatg tattatatac
    26041 agaacaagag gcgaagttag tggctcagct tccaataaag ccttttagag taaaaacagc
    26101 agctaaaatt tggagtgtaa gtgaaagtga agcatataga gtattagata aacttgctag
    26161 caaggctctg atactagata tagaagataa taaagggaag aaatatataa tgcctcctcc
    26221 aatggctggt ttttttgagt ttgccatgat gagaacgaga cacgatatag accaaaaact
    26281 tttagcagaa ctttactatc aatatatgaa tgtagaagaa gattttatca aagatttatt
    26341 ttattcaaca gaaactaagc ttggaagagt atatgttcaa gaggaagttt taactaatga
    26401 taatgaggtt agtattttag attatgaaag agctactcac ataattgacg aatcaactca
    26461 tataggtata agtatgtgtt actgtagaca tagaatgcaa catgttggaa aagcttgtga
    26521 tgctcctatg gatatatgta tgacgtttga caatgtagca aattctttga taaacaataa
    26581 atttgcaaga cgtgtggata aagtagaatg taaagaacta cttcatcaag catatgagca
    26641 taacttagtt caatgtggag aaaatgttag aaaaggggtt acgtttatat gtaattgctg
    26701 tggatgttgc tgtgaagcaa tggtagcagc taaaagattt ggaaatcttc atccagtaca
    26761 gacaacaagt tttataccaa atataaacca cgaaagttgt gtaaaatgtg gtaaatgtat
    26821 agctgcatgt ccaatagatg caataagcaa ggttaaggaa gatggtaaag aatatataaa
    26881 aattgatgag gatagatgtc taggttgtgg agtttgtgtt agaaattgcc ataaaaatag
    26941 cattatgtta ttaaagagag atgaaaaaat aataactcca gcaaattcag tacatagagc
    27001 agttctcatg gcaattgaaa agggtcagtt acaaaattta atttttgata ataatgcttt
    27061 agctagtcat agagctatgg gagctatact atcagctata ttaaagcttg aaccagctaa
    27121 aaagatttta gctagtaaac agttaaagtc agtatattta gataaattgt taagtatgaa
    27181 tgataaatag aaactaactt aaaaaagaca aggtttttag gcttatctac atcttaataa
    27241 acttattatt taatatctaa atttaatttt tacacatata aaacttataa ttgaaataaa
    27301 aaaagactat attcaattaa tattacttga atatagcctt ttttagttat gttaagctat
    27361 ttcttttatt tgtttaaatg agttttcatt tccatttcca ccaatttgtg ttatagattt
    27421 aaatctcata gttttaaata gttctttttg gttataatct agtgagtttc ctactagtat
    27481 tacaggagat ttagtttttc cagccaaaac tccaacagat aatccatcta ttaaatcatc
    27541 ttgtttattc attccatttt tagcaacata taagttatct atcttagaat ccttgtaaaa
    27601 ttcttttatt acttttgcat tagtttcatt tctagtgcta cctgatattc tctgtggatt
    27661 ttgaagtttg ctttctatat ttttagaaac tgtatattct ccaccgatta cataagattt
    27721 atcaattttt ttgtttttaa ataagttatt tatttcagtt atattactat tttcattagt
    27781 aagtattata ggcatgtcat tttgggctga tacagctcca atgcttactg catcagctaa
    27841 tcctttttca ccattaacta ctgaaatttt tgatatatta ctaatattat tcaattcttt
    27901 agcaatgttc atagaagttt gatatctatc acttcctgaa attctagaga cagagatatt
    27961 atttgatttt atagtatcta gagaattttc atttatagaa gcctctccac caactacata
    28021 tacattttta acttttaatc tctttaattc agccaaagtt ttttcattta tttgagagct
    28081 tccagttaat aatataggag catttttctt gtatgcaaat ggtgttgcag ccaaagcatc
    28141 tgcaattgct gagtcgttta ttaaaactgc attttcagca ttttcccaac ctgcttgact
    28201 tatcttaata gaagtttcat atctatcagt tcctgtaagt gcacttgcat ttacttcgtc
    28261 ttgatatgta acttcaaatc tagcatttgg acttcctaag aaaacaatta cgcctccttt
    28321 tggcaatcta actctattgt tctttgtaac taaagaatag tttacaggaa ctcctttatc
    28381 atcataaacg gcaaaagatg aattttgagg aagattaact tctatttttt tatttgctat
    28441 atcatcacca atcttatacc attttgcata accattagat gctagttcgc aagtaaaaga
    28501 tttttctgct ggcaaattag ttatagaatc ttcactaaca tatgtttgtg tggcaaaaga
    28561 taaatactct gtaccatttt ctttataaag tttaatatcg gataaatcac gacctattac
    28621 accaggtatt tctataaaag catttgaatt attctcatct attatcttag tattaactat
    28681 ataacctgga gtttcatcag aaaggccagg agttgccttc acagaaccaa acatatagct
    28741 ttgagaagta tatttttcat ctactagata gtaaccttta ccatttcttt ttttccatac
    28801 ttcttttaca ctatttgata tattgttatc atctaccttt tgagctacat agtataaact
    28861 tgctgtttga cctaatccag ggacatcatc ataagagctc atatttagat atgtaatatt
    28921 atttttttct tttacaaatt ttaaacaact attacctttt tcagatacaa atctatcttg
    28981 acctatgtat atgtatttat cttcaggtcc attttctata taaggagaag ataccgttaa
    29041 agtaccatta tcattaacat caacttttac catatttgaa gaagcataaa gcccactatt
    29101 ttcttttaaa tgacttggca ttttaacttg ttgtggctta ctaaatgttt tatcaggttt
    29161 tatttcttta atttttccct tttcttttaa ggcagacaat aagatttctt gtccaattat
    29221 ttcatcaaat tggctatttc caccagaaga aagaactgca actgccatat tttcatctgg
    29281 aagaactata agattactgt ggaatagtaa agaatctcca ccttttgtaa gagcttttaa
    29341 attgtactga ttaaatggat aagtattgac acaatcccat cctaaaccat atccaagtat
    29401 agaatcttct ccttcaggcc atagaccatt taaatattct ttgttttcca tagcttttac
    29461 tgaagcagga gaaagaatac catttgagtt tttcataaat gtttgtgcaa atgtacatag
    29521 attttctgca cttgaatata aaccacctgc ccctatagca ttaaggttat cttgtggaac
    29581 tgcatcctcc caataaggta catatgcttt tgctagtttt gaactatcaa aactattttc
    29641 tgtagttttt gtattttgta agttaagtgg attatttatg tatttgtcaa gaaaattagt
    29701 aaatgacata ccagatactc tttctactaa aatttcagct aaagtaaatc catcattaca
    29761 ataaacagaa aaagctcctg gttttgcttt taatctttgt ttttgtaatt cttttaagaa
    29821 gttatcatga ccataagaat cattgtctcc taataagagc gtatttttaa agctgcttcc
    29881 catcaatcct gatgaatgat ttaataacat tcttggtgtg atttccttgt atctattatc
    29941 agccatttta aattcaggta tataatttac aacaggagta tctaaattta cttttccatc
    30001 atcaaccaac ttcatgacag ctgttgtagt aaacatttta ctgatagatg cgatactata
    30061 catattatcc ttagttaaat ttttgttatc ttgtttgctg taaactccac cattaccaga
    30121 aatctcaatt ttaccattat caatcaaggc atattgagca tttacaacac catactttga
    30181 tagcaaagtt tggattttct ctttagcaag agctttagtt ttcgtgtaag attccgagtt
    30241 tttactataa tcatgtaatt ctatatcaga actttgatat ttttcggaat tatcttttgt
    30301 attatcagca aaagtaatat ttggagcagc agtaaataac atactcaata ctaatgtact
    30361 agataataat ttagagagct ttcgcttaaa catattacat tacctctttc cctttttaat
    30421 aatttataat tagcataaca ttcttaatta ttttaatcaa cagattatta tcaataaaaa
    30481 aagcttttat aagtatagct ttttatataa ctgagaaaaa tataaaaata atatttgttg
    30541 ctgtataaag aataaagggt ataggtaata tataaataaa aatggaggaa ttttagatat
    30601 atgtacaaga atataaataa gaatcaactt aaagaaatga tgaaaaatga aaaggatatt
    30661 ttattattgg atgtaagaac taaagatgaa tttagggaac ataaaataga aaatgctata
    30721 aatatatcat tacaagagtt aatcaataat atagatgaaa tttacgagta taaagataag
    30781 aaagtagtgg tttattgcag aagtggtcat agaagtgtta ctgcatgtaa tttgttagct
    30841 gaagaaggtt ttgaacattt atataattta aactcaggaa taattgatta catgaattaa
    30901 ataatataag ttatgcatta aatactatat ataattctgc ttattaagta ggaatatctt
    30961 tagatattta taagatagat ttaggaaaga acgaattttt atactttttt tgaggcatat
    31021 tctgtagtaa atattagttt ttaagttgaa aaaaggtagt agctaaaaag ctactacctt
    31081 ttaatatgaa cttaaagtat tgttaaaaaa tatatttaaa tacagaatta attttttaat
    31141 gaccagttaa gttactatcg ccaaatttat cattaaatgg ttttataatt ctatttatag
    31201 gtcctttaag aaggacattt aaacaaatag acagaatcat aaatagcaat agaactagta
    31261 tatctttagt tagatttgga gcatatatac caccaactgc ttcccttgta gcacctattg
    31321 cataagtaaa tggcatgata gggtttattc tttggaagaa tgtaggtgta acttgtatag
    31381 ggaatgtacc accagaagaa ccaatctgaa cgactaatag tactattgca attgcttttc
    31441 ctacatttcc aaatacagaa actaaagaat acactataaa gttaaatacc atactagtaa
    31501 acataagcaa aattatcaat aaaattggat gttttgctgt tactcctaat aataatatat
    31561 ctccagttcc gattataaaa ccttgaacaa gagctatact taaaaaagta agacctcttc
    31621 caaagtaaat ttcataaggt ttatagtttc catgtacaga tgtagatagt aatgctgaaa
    31681 taagtaatat accaacccat gtagaaagga cactataaaa tggtgtcatt gcagaaccat
    31741 aattttcaat tggatataat ttttctgttt tcacttcaac aggttctttt aagaaattag
    31801 attttgtcaa tatatcactt tcaagcaagc tgactaattt tttcatatct tcaccattac
    31861 taattttgtt taaagtttca atcaaatcat ccaacatccc ttttgctaaa ggaagtcttt
    31921 ctcttattaa tgatatactt tcctgtgcat ttcctgaaag ttttagagat gtagtaagta
    31981 tttcttcaac ctttggaagt tttgattctg ctttatctaa aacagtaatt atatcatttg
    32041 ctacttttat actgtttgca aatatactat ttataggttt tgatattttt gagtcaaaat
    32101 tatttaaaat atttaaatta attcttccaa taccatttga tagacttaaa acattattca
    32161 aagcacttac agatggttgt tgtccagata taactttgtt tttaatatca tttaatgttg
    32221 atatagatga atctatttta ttgctagagt cttcaagatt atttattaca tcatccaatc
    32281 tattatttga tgtaagttga tttaatttag ttaaaaaatc caccaaagta tcgtttaaag
    32341 attgtaaatt agaaagtttt tctgataaat tatctatcaa cttaggtaca tcttcactgc
    32401 cactattaac tgcatcaatt aaatttaatg taagagaaga agcagaagac gaaaggtcaa
    32461 ccattaaatt taagtcagac ttaataagag gagaaagttc atctaaatta tcatttgtat
    32521 cctctaagaa tttctttaag tcacttgata acagttttgt atcattcaaa gtctttttaa
    32581 ttgtaggcaa atcagattta atatctttaa ctgtatcaga taattttgaa gtagcatcag
    32641 aagctaaatc aacagtctta tttatatggt caaatctctt ttgaaggtca ataagggaat
    32701 tttcaacctt tgttaacttt ggaagactgt gttcgattcc tgcaccaact ccgtttgata
    32761 taccaagaac gatttcacta actgttttta ctatagtctg attaacctgt aattgtattg
    32821 tagaagcacc tttatctgta atctttggtg caactgcatt tattttttca ttaacagtat
    32881 agataatttc gcctttttta acatcatttg taattaaaga tgtcaaatct ttagtaaaat
    32941 tttttggaat cttaagtgat gcatagtaag tacccttctt aacaccttca agagcagttt
    33001 tttcatctac aaactgccaa cctaaacttt tgttatcctt aagttgttca attaattggt
    33061 ctcctacatt aatcttttgt cctgctattt catttccttt atcttcattt gttactgcta
    33121 caagcatatt agcagtgctt ccataagggt cccaagatgc ctttatattg aaccaagcat
    33181 acagagatgg taaaaaagca agacctaaaa ctattacaag tactgcataa ttaccacgta
    33241 tatcttttag gtcactttta aaaatttcta atatattttt taaacccttt gaaggtttta
    33301 aattcttgat attattccag tttaaaattt cttttaaact attagttttt ttcaaatgta
    33361 tcacctccta aaatattttg ataaagaaaa aagttagatt tataatcaat tttaaattat
    33421 attacttttt acacaaatag taaagttaaa attttgtc
//
LOCUS       DAMJVM010000037        32793 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.037, whole genome shotgun sequence.
ACCESSION   DAMJVM010000037 DAMJVM010000000
VERSION     DAMJVM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32793
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.037"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(216..731)
                     /locus_tag="PC523_001832"
     CDS             complement(216..731)
                     /locus_tag="PC523_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HDF2475357.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDVAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(1094..2164)
                     /gene="leuB"
                     /locus_tag="PC523_001833"
     CDS             complement(1094..2164)
                     /gene="leuB"
                     /locus_tag="PC523_001833"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDF2475358.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVEVCRKNEAVLLGAVGGPKWDKCKGDTRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVDKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     ETYNENEIRRIGKRAFEIARNRSKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVREPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLRDDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEYAVQSILNKGYRTID
                     IHNGVGTVVGTKEMGELIVNEI"
     gene            complement(2171..2662)
                     /gene="leuD"
                     /locus_tag="PC523_001834"
     CDS             complement(2171..2662)
                     /gene="leuD"
                     /locus_tag="PC523_001834"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDF2475359.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIEEGDKIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(2677..3954)
                     /gene="leuC"
                     /locus_tag="PC523_001835"
     CDS             complement(2677..3954)
                     /gene="leuC"
                     /locus_tag="PC523_001835"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDF2475360.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDIVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGKIGVDGALYKSMEFCGEGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTIEYINSHKCTTITKDVNVYQADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILASGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(3911..5653)
                     /gene="leuA"
                     /locus_tag="PC523_001836"
     CDS             complement(3911..5653)
                     /gene="leuA"
                     /locus_tag="PC523_001836"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDF2475361.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESVKGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMSTPNI
                     YADQIEWFCENVSCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIREIYEDCTKLIVHDRHPYVGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKIESDRIGEELSSEAIFNLFRKEYIDVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARVSYMGNEQRIVGVGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEVK
                     NGDDYDAENLSQTC"
     misc_binding    complement(5777..6023)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6171..6617)
                     /locus_tag="PC523_001837"
     CDS             complement(6171..6617)
                     /locus_tag="PC523_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475362.1"
                     /translation="MSKNKFIYMNYYDNGYFKRGMAIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLSALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(6638..7357)
                     /locus_tag="PC523_001838"
     CDS             complement(6638..7357)
                     /locus_tag="PC523_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDF2475363.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASNKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYHGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGVRVILGTDSHICYSIGKFENAEKVLKSVDF
                     PDELVINYHEDEIIGFFDINF"
     gene            complement(7475..8749)
                     /locus_tag="PC523_001839"
     CDS             complement(7475..8749)
                     /locus_tag="PC523_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDF2475364.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDIRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RVKAAKEELFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            complement(8949..10316)
                     /locus_tag="PC523_001840"
     CDS             complement(8949..10316)
                     /locus_tag="PC523_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2475365.1"
                     /translation="MENSSTNLGNESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIIMACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWIFGASESTIGYSMDYLNIYLMGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIVLPVLGLGISPFIMQSTESLVLISLNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCLTYSTILWL
                     LLMIFPTFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIEE"
     gene            complement(10566..11030)
                     /locus_tag="PC523_001841"
     CDS             complement(10566..11030)
                     /locus_tag="PC523_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HDF2475366.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHILQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(11035..11664)
                     /locus_tag="PC523_001842"
     CDS             complement(11035..11664)
                     /locus_tag="PC523_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HDF2475367.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMVPLKKAKEAIFYPIVDFSEEDLKSFFDDCIMKKINESIKKVIYKNKEGY
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKNGEVIEQINS"
     gene            complement(11698..12585)
                     /locus_tag="PC523_001843"
     CDS             complement(11698..12585)
                     /locus_tag="PC523_001843"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HDF2475368.1"
                     /translation="MKQTNTNIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLLLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSAYILF
                     VVLSLISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRGDAVEHRKILEDYDVENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNESNPTDIILTDN
                     ALKINVILWIITCLIILIF"
     gene            complement(12614..13852)
                     /locus_tag="PC523_001844"
     CDS             complement(12614..13852)
                     /locus_tag="PC523_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475369.1"
                     /translation="MDGDILMSKILKYKNEIFLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLLTFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGAGIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNIYNIFLSLLLVTII
                     ISNINGFIEILNIGIKSYPV"
     gene            complement(14236..15378)
                     /locus_tag="PC523_001845"
     CDS             complement(14236..15378)
                     /locus_tag="PC523_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2475370.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLGGGTIPKYNKELSF
                     LSKDDESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHAIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANTVKV"
     gene            complement(15519..16385)
                     /locus_tag="PC523_001846"
     CDS             complement(15519..16385)
                     /locus_tag="PC523_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2475371.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVSR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRVGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLFGVKLGFPVIIESIVPIIISVFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEKSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            complement(16663..18210)
                     /locus_tag="PC523_001847"
     CDS             complement(16663..18210)
                     /locus_tag="PC523_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2475372.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKLVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVNKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMRHTPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVLNMLSGKINEETMRELNYQVDELGKSPEDVAHSF
                     LVKEGLV"
     gene            complement(18203..19339)
                     /locus_tag="PC523_001848"
     CDS             complement(18203..19339)
                     /locus_tag="PC523_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2475373.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSGEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDVDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            complement(19602..20699)
                     /gene="dinB"
                     /locus_tag="PC523_001849"
     CDS             complement(19602..20699)
                     /gene="dinB"
                     /locus_tag="PC523_001849"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDF2475374.1"
                     /translation="MELHRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLKK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLNIDEEETRNILDELCEEVCH
                     RLKSSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRRGIDNLLRNVEMNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            complement(21095..22600)
                     /locus_tag="PC523_001850"
     CDS             complement(21095..22600)
                     /locus_tag="PC523_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDF2475375.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHEHKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAAV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            complement(22861..23211)
                     /locus_tag="PC523_001851"
     CDS             complement(22861..23211)
                     /locus_tag="PC523_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475376.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGKVNEAEEILFDKAKTVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            complement(23433..23624)
                     /locus_tag="PC523_001852"
     CDS             complement(23433..23624)
                     /locus_tag="PC523_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475377.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            complement(23742..24656)
                     /locus_tag="PC523_001853"
     CDS             complement(23742..24656)
                     /locus_tag="PC523_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2475378.1"
                     /translation="MKKIILKDILFLVTTLSLIVILLIVVNITANGKIKSTIKSYLKS
                     DKNSVSQNDIQSSTKNTEFKTTILNTGKSDCILVEIGDKVIMIDSGEDKNGKQIVDRL
                     KEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDSLKKA
                     DIEPVLLKENMNIVINSAEINIQSALKSKYESSNDYSIITSISYGAHKFLFAGDAEEK
                     RLSEFINGNTLKYDFVKIPHHGRYDKLTEAFLESISPKYAVITCSEKKEPDEEILKIL
                     EKLNIKTFLTSNGEVVINSDGKTLNVNQ"
     gene            complement(24790..26286)
                     /locus_tag="PC523_001854"
     CDS             complement(24790..26286)
                     /locus_tag="PC523_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2475379.1"
                     /translation="MFEFETQLKRLKHEVLTAVAKLSIEENLTKENIQKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVGLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFIDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKIFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKCLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSIQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEGVNLER"
     gene            complement(26566..28056)
                     /locus_tag="PC523_001855"
     CDS             complement(26566..28056)
                     /locus_tag="PC523_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2475380.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEENEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEEKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMENGEDFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            28523..28723
                     /locus_tag="PC523_001856"
     CDS             28523..28723
                     /locus_tag="PC523_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2475381.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
     gene            complement(28903..29781)
                     /gene="speB"
                     /locus_tag="PC523_001857"
     CDS             complement(28903..29781)
                     /gene="speB"
                     /locus_tag="PC523_001857"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDF2475382.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVYEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFEKIVNMLNGKNIYITIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISEKIK"
     gene            complement(29771..30622)
                     /gene="speE"
                     /locus_tag="PC523_001858"
     CDS             complement(29771..30622)
                     /gene="speE"
                     /locus_tag="PC523_001858"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDF2475383.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKKYMDICSCKLDDERVNLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            complement(30652..31065)
                     /gene="speD"
                     /locus_tag="PC523_001859"
     CDS             complement(30652..31065)
                     /gene="speD"
                     /locus_tag="PC523_001859"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDF2475384.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            complement(31152..32627)
                     /locus_tag="PC523_001860"
     CDS             complement(31152..32627)
                     /locus_tag="PC523_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2475385.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITFEKV
                     RKEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
BASE COUNT        10659 a         5918 c         3421 g        12795 t
ORIGIN      
        1 cttttcataa gtctgaatat tctgaattaa ttcctcaaaa aaaatatact ttttatctat
       61 ataaattaat tttttcagta tataaattta atgctattac gataaatctt tttagtataa
      121 ctaatttatt tttatcttgt atttttattg tatttttgaa ttttattttt tataaatata
      181 aaatttatgt ttgaaaatat aaaattttta tctaatcaaa gttcaatata ttccactttt
      241 tctttcgatt cttcaaagaa ccattcttca ttagaaaaat caccttctgc aatttgtcca
      301 ataggcattt tcatatactt tccagttgct gttactgcta catctccatt tggtagaatt
      361 atttctcctg taccttcaaa taattttctg ttttctttag ttattcttcc aacaactttt
      421 atagtagcat ctgttggtac tggtttctta tatttcaatt ccaaagacac tgttactccc
      481 catatttggt catcatttat acttacagct cttccaatag tctcgtctaa tattgcagca
      541 gacatacctc catgaactct tccaggataa ctttgatgcc aatcctttgt attacaaatt
      601 gatacaagct ccccattttc aagttcataa aaatctgctt taagtcctaa ctcattttgt
      661 gttccacaaa ctaaacaaga tttgctacta ttttgcttct ttaaaacttt atactttgcc
      721 aaaaaatcca tctcctaacc gacattagta gtcgcttaat attaaataat tctaaaatat
      781 attatataat attaataaaa tccctgcaaa cttctaataa taaaaatagg ctatttcaat
      841 atgataagtt taatttaaag tattattttc taaaaaataa ctttatcaaa atagaaactc
      901 aaaatagata taagcctctt tttcgttatt aaaatattaa aaatatattt gacttaatat
      961 aaaaaagagt gcctcattaa ctaataaagt ttattttgag acacccctca taaaacaaat
     1021 tatactataa tagatttgta gcaatcttcc aagttatata tttaaattat attatacaaa
     1081 ttaaactata attctaaatt tcatttacta ttaactcgcc catttctttt gttcctacaa
     1141 cagttcccac accattatgg atatcaatag ttctataacc tttattaagt atactttgta
     1201 cagcatattc aatacactta gcttcttctt ctaggttaaa actatgtctt aacatcatgc
     1261 ttacagatag aatagttgca attggattta caatatcttt tccggctata tcaggtgcac
     1321 ttccatgtat tggttcatac ataccaaaac tatcgtctct aagtgatgct gatggtaaca
     1381 tacctataga acccgtaatc atagatgctt catcagatat tatatctcca aatatattat
     1441 tagttactat tacatcaaat tggctaggtt ctcttacaac ttgcatagca gcattatcaa
     1501 catacatatg tgatagctct acatcttcaa actctttagc taaatcttcc attatacttc
     1561 tccaaagtct tgaactctca agcacatttg ccttgtctac acttataagt tttttacttc
     1621 tatttctggc tatttcaaaa gctctcttac caattcttct tatttcattt tcgttatatg
     1681 tttctacatc ataagcattt tcaataccat caataatttt tcttccacgt tctccaaaat
     1741 aaatgccccc tgttaattct cttacaacaa caaaatcaat gcctttatca actatatccg
     1801 ttctaagtgg tgaagcttct tttatacttt catacattgt ggctggtctt aagtttgcaa
     1861 ataaacccaa tgactctctt aattttaaaa gaccagactc tggtcttgta tctcccttac
     1921 atttatccca ctttggtcct ccaacagcac caagtaatac agcctcgttt tttcgacaaa
     1981 cttcaacagt tttattaggt agaggttctc cagtttcatc aattgcacac cctcctgcta
     2041 atacatattc acaatcgaat ctatgattaa atttacaaca aattttattt agaaccttta
     2101 taccttcatc tataatttcc ggtccaacac catcaccttt tatgactgct atattacaat
     2161 tcatactcca ctacccccta tttgctttta ttgagttaat taagccttca tttaatataa
     2221 tattttgcat aaactctgga aatggttctg ctttatattc ttttcctttt gttatatttt
     2281 ttatcacacc tgtggaaaaa tctacttcta tcttatcccc ttcctctatg ttatttgcag
     2341 cttcttcaca ttcaagtata ggaagcccta tatttataga atttctaaaa aatattctag
     2401 caaaagtaga agcaatcaca caggatactc cactttcttt tattacaatt ggagcatgtt
     2461 ctcttgatga accacaacca aagtttttag ttgcaactat aatatctccc ttttttacct
     2521 tagagataaa tttatcatca atatcctcca tacaatgtgt tgctagttct ttataatcag
     2581 ctatatttaa atatctagct ggtattatta catccgtatc tatattatct ccgaatttaa
     2641 aaacacttcc gttagcaatc ataaatttta cctccattaa attatttctg atggctttga
     2701 tatttttcca gttatagctg atgctgctgc tacagctggg cttgcaagat atacctctga
     2761 atcaacatgc cccattcttc ctacgaaatt cctatttgtt gttgaaatag acttttcacc
     2821 tgatgcaagt attcccatat gtccccctag acatggtcca caagttggtg tagaaactgc
     2881 tgctcctgct tctataaaag tagtaatata tcctttttct atagcttcaa gatatacttt
     2941 ttgtgttcca ggaaatatca taactctaac tccatctgct acttttttgc ctttcattat
     3001 ctcagctact acttcaaggt ctgatattct tccatttgta caggaaccta ttacaacttg
     3061 gtctatcttt atatccttat caatttcatc tactgttcta gtattttcag gaagatgagg
     3121 aaaggctacg gtttctttta gttcagctaa atctatttcg tatacttcat catagactgc
     3181 atcttcatca gcttgatata catttacatc ttttgttatt gttgtacact tatgtgaatt
     3241 tatatattct atagtcttat catctactgg aaaaattcca tttttagctc ctgcttctat
     3301 tgccatgtta caaatagtaa acctatcatc catagataag tattctactc cttccccgca
     3361 aaattccata gacttataaa gcgcaccatc aactcctatc ttacctatta tatgaagtat
     3421 tatatcttta ccacttatcc atttatttgg tttattcttc aaaacaaatt ttattgctcc
     3481 aggaacctta aaccaacatt tccctgtagc catacccgct cccatatcag ttgaacctac
     3541 accagtagaa aatgctccta atgctccata tgtgcaagta tgagaatcag cacctataat
     3601 tacatctcca gcagttacta tacctttttc aggaagaaga acatgctcta ttcccatatc
     3661 tcctacatca aaaaaattta caactccttt tgactttgca aaacttcttg taaatttaca
     3721 ttgttctgca ctctttatat ctttgttagg tgtaaaatgg tctaaaacca tcactacttt
     3781 agtcttatca taaacatctt caattcctaa tttttcaaat tctctaatag ctactggtga
     3841 tgttatatca ttacctaaaa ctatgtctaa atctgcttca attaactgac ctttcttaac
     3901 ctcatttaac ttagcatgtt tggctaagat tttctgcgtc atagtcatcc ccatttttta
     3961 cttcctcctc aatataactt ttatttattg cattcataag tgcattaata gctgctgtat
     4021 ttatgttttc tgatatactt acaccaaaat atacttcatt attgtgctct attcctacat
     4081 aagctacccc tcttgaatgt gacccttcct ctaatgcatg ttcccaataa tatctaaatt
     4141 tataatcttt cattccacat tgctttagtg cattattaaa tgaatctaca ggtccatttc
     4201 ctacaccaac aatcctttgt tcatttccca tataacttac tcttgcagtc atctcaatca
     4261 tgttagtatc attagaatca tcattttcat agtttaattc atccattgat tttattttat
     4321 atttcttaac cttataggga ctctcaacat caatatattc tttcctaaat aaattaaata
     4381 ttgcttcact actaagctct tccccgattc tatcagattc tattttgact acatttccaa
     4441 aatcagaatg catattctta ggtatcataa atccataatc tgtttccatt atataaacag
     4501 ctcctccttt tccagattgg ctatttattc tgataatttc tttgtactct cttccaatat
     4561 catgagggtc tattggtaaa tacggaactt cccagtaatc attatctctg tttttcattg
     4621 ctatcatgcc ttttctaata gcatcttggt gtgaacctga aaaggcacaa tgtacaagat
     4681 tacctacata tggatgtcta tcatgtacta taagttttgt acagtcctca tatatctctc
     4741 ttatcttatc tatattacta aaatctagtt ctgggtctat tccttgtgtg taaagattaa
     4801 gtccaacatt tactatatcc atattcccag ttctttcacc atttccaaat agcgttcctt
     4861 caagcctatc tgcaccagct aataatccaa gttcacttgc agctgtacaa gttcctctat
     4921 cattgtgagt gtgcaaactt agtataatag aatctctaca acttacattt tcacaaaacc
     4981 attctatttg gtctgcatat atgtttggtg ttgacatttc aactgttgat ggaagattta
     5041 ttattacttt tttttgtgga gtcggtttcc aaacatctat cactgcctca catatttcaa
     5101 gtgcataatc catctctgtt cctgtaaaac tctctggtga atattcaaaa gtaaattctg
     5161 tctctggata tttttttact tcttcattaa ataattttgc cccttcaact gctatatcta
     5221 ttatttcttg tttactcata ttaaaaacca catctctttg aagaacagat gtagagttat
     5281 ataaatgtat aattgctttc ttacatccct ttactgattc aaatgtcttt tctataagat
     5341 gagccctgga ttgtgtaagg acttgtatag ttacatcatc tggaatcata ttctcatcta
     5401 ttaattttct taaaaatgta tactctgtat ttgatgctga tggaaatcct acctctattt
     5461 ctttaaagcc tgtatcaata agcattttga acattctgac tttttcattt acactcatag
     5521 gtgttggtag tgactgattt ccatctctta aatcaacact gcaccaaatt ggagccttaa
     5581 atatttcatt atctggccat ttacgattta ctatttgcat tttatcatat tttttatact
     5641 ttccacactt catattattt cccccttttt tgtttttttg tattcagtat accttatatc
     5701 gacgcaatac tgcacaattc ataagaataa atgtcaattt tatcattaga ataaaaataa
     5761 cctcatctct aataaaatta gagacgaggt tttgctcgcg gtaccactct atttagcttt
     5821 ctaaaaagct cccttagtaa tagccaatac aataattata atgactatat ttcctataac
     5881 gtggaattac tccgattatt ctaagattta aatcttagaa aacagctcct agacaagttc
     5941 agaactatca actgctaaat cacaccaccc tttagctctc tgaaagtttc atggttctta
     6001 ctacttctat tcatcacttt ttttatcatt taaattttct aaatattttg atttaatagt
     6061 tattatatac acattaaaat atattgtcaa cacaaaaaat aattatctcg attttttatt
     6121 tttgataagc aattctataa aacatgctaa ctattgaaaa cagtatgata ttatgcctta
     6181 aactttttca tcttttcatc tatgatattc aataattttt tttcatcttc caaactcaat
     6241 gcacttaatc taattttata attagattca tcagtgtata tatttaatat attttttacc
     6301 acttctattt tttctattat cttataatta aacttttggc tttgtttaga ttttatactg
     6361 aatatacatt gattatttat taatattgaa tgattatcga gagtaatata cacttttccc
     6421 tcatatttat ttgaaaaatc acgaccttgc ttaaatagaa aaactgctgt tattatacat
     6481 aaaatatata atattgtcgt tgaatatata aaaattgtgg ctaaaattat aagtgccaaa
     6541 accatatata tagccattcc tcttttaaaa taaccattgt cataataatt catatagata
     6601 aacttatttt tactcaaatt ttttcactcc caatattcta aaaatttata tcaaaaaatc
     6661 ctataatttc atcttcatga taatttatga ctaattcatc tggaaaatct actgatttta
     6721 aaactttctc tgcattttca aattttccta tagaataaca tatatgagag tcagtaccaa
     6781 gaattactct tacaccatat gttttacaca atgtcaacat atgacttaca ttttcccaag
     6841 taccagttct atgagagttt gaacttaatg atgaattatt tatttctaaa aggatatttt
     6901 tatctttagc tttctttact ataggttcat aatctaaagg atacctactg tcatctggat
     6961 gtccaattat ttttacctta ggcttatcca tgacttttaa aatagcattt gtattttctt
     7021 cttttgtacc tgaagctata caaggtctat gcaaacttgc gattatataa tccaaatgtt
     7081 gcaacatatc ttcttgaaca tctaaattac catgataatc cattatattt ccttctatcc
     7141 ctctaagaat ctttacacct tgcacttctc gtgggagcaa atgcaaatta taaaaataaa
     7201 atggatgtgg ccctcctggc atattaggtc cgtggtctga tattcctaag tattttatat
     7261 ttttattact cgcctcttcg acattttcct tgaaagtact atgtgcatgt ccacttatta
     7321 gagtgtgaca atgtacatcc ataatagact ttttcattaa atttcctcct aatgttttac
     7381 ataaattcgt gaaaatagct aaagggctat ttcaaaacta aagctgtcat aacagacagt
     7441 gtaacgtttt aaaacagccc cattttcaat tattttacac ttctaaatct ttaaatttat
     7501 ctttaaataa ttctccaata cttcctaaag attcctctac ttctaagtat tctgttatat
     7561 cctcttctgg ctctctagtt gcttctttta ttgaaaccaa cattctttta gtctttggtt
     7621 taaaatcaag aatcttaact ttaacctcat ctccaatatt aacaacatta gtaactttag
     7681 ccactctagt atcacttaat tcagatattg gtaaatacgc atctacacct tctttaattt
     7741 gaacaaaagc ccctttttct attattctaa gaactttagc attgacaaca tcatcaatct
     7801 gtacttcttt tgctattaaa tcccaagggt cattatttat gtcttttaat gccaaggcaa
     7861 tttttttagt ttctttattt acatccaaca cataaacttc tacttcttgt ccctctgaaa
     7921 caacatcttc aacactttca actcttgtcc aagctaaatt gtttatatgt gctaatcctt
     7981 caatcccacc aatatccaca aatgcaccat aaggcattat ctttgttacc ttaccatctc
     8041 ttttctgtcc aacttcaaga gagttaaata attcttcttt agctgctttt acacgagctt
     8101 tttcttcttc tctttcttgc ttaattcttg ctctttcagc ttctttttcc gccttaattt
     8161 gagctcttct ttcttctctc tctttattta tacgctcttg taatacatct ctatgagatg
     8221 ctacaagtct attttttcta atatccaatt cttttatata aacttctaag ttaagaccaa
     8281 caaatttaga tgtgtctgta ataaattttg tatcaagttg agaaattggt ataaaaaatg
     8341 tatatgtatt gtaattagca aatacacctt tatctatatt cttttcaaca tttacagtta
     8401 ttattctatg ttcatcataa gctttttgta actcagcaaa atcatttctt ttgtcaagtt
     8461 gtaatttaga taaagttatt gtcccatctt tttgagatac ttttgttatt acagcagtta
     8521 tttcatcacc tatatggtat atatcttcta tatattgatt tttttctatg ttcaattcac
     8581 ttataggtat tattccatca aacccatagt ctaatcctaa ttgtacttca tcatttctca
     8641 ctgcagttat ttttccagtt gtcaattctc caactttcac tttgctgaat acttgctctt
     8701 gctggtcaag taattcttgc attgttaaat cattttccat tataaacatc ctttcctctt
     8761 attgattatc atatctgtaa atattatacc attaatattg caaatactta catttttctt
     8821 actataattt ttaaaataaa ttcagcttca tatatttata cattatagaa aatatcttat
     8881 taaattaaaa tacaaattct aatttttatc taataaaata ttttcataaa aaatttatat
     8941 tgtatatctt attcttctat tgctttaggt tttgatagaa aacatttata aaatataaag
     9001 aaagaaattg ctgttatagt agctgctgtt atatccgcaa ctggctctgc taaaattact
     9061 gcaaaaagtt tttcattaaa gaatgttgga agtataaata ttaaaggtat taaaagtaca
     9121 atctttctaa gtagagctaa aactaaagat attgttgctt ttccaagtgc taaaaatgtc
     9181 tgttgacaag ctatttgaat cccaaacata aaagcaccag caaaatatat ttttatactc
     9241 caactagtca tactaagtaa ctgcggattc ttattaaata tactaacaaa aaatgtagga
     9301 aatatcatta ataaaagcca caaaatagta gagtatgtaa gacatgataa tagacataac
     9361 ttaaatgtct tttttactct atcaagttga cctgaaccat aattgtaact tattattggt
     9421 tgtgctcctt gtgtaacccc catattagga agcataagaa tttgcattat actgctcatt
     9481 atggccattg agcctactgc taaatctcct ccatacattt gtaatttact atttaaggat
     9541 ataagtacta aactttctgt agattgcatt ataaatggag agattcccaa gcctaacact
     9601 ggtaagacga tacttgcttt tggtatcata tacttctttt ttattttaag tatgcttttc
     9661 tttccaaaaa gaaacataag cacccaaata gcagaaactc cttgtgcaat tatagtagct
     9721 aatgcagcgc cttttacgcc catattaaaa ccaaatataa aaatcgggtc gagtattatg
     9781 tttatcaaag cgcctatagc aactgtcatc attccagttg tagcaaaccc ttgggtattt
     9841 ataaaagtat tcatacccat tgaaatctga acaaatactg ttcccattaa ataaatgttt
     9901 aaataatcca ttgaatatcc aattgtagat tcacttgctc caaatatcca aagtaaaggc
     9961 tcttttccaa tcttaaaacc tatagtcaaa agtattgcta aaattaccag tacagaaaaa
    10021 ctgttactca ttattttctc tgcctcatca ttgttttgct cccccatttt tatagcagct
    10081 aatggacctc ctcccatacc aattaaataa ctacaggcca ttattataat tattattgga
    10141 aatgccactc caacaccagc catagcaact tctccatttt ccattctacc aataaaaatc
    10201 ctatcgacta tgttgtataa aacatttaca atttgtgcga taatagcagg tgtggcaagt
    10261 ttaaataata atttacccac actctcattc cctaggttag tgcttgaatt ttccatttta
    10321 aaacctcctc aatccaataa ttaaaatata tcactaaaat tttattctct ataaaacttt
    10381 cactttatca gcagacaaat ttagagtgct ttccctacaa gtaatactat actatatttc
    10441 ttttgccata gtaaacattt tagaactaac tgaatttaaa tactttatct cactttttat
    10501 ttgttcttaa aaatttttca catgtactca taaaaaaact atccaagtat attacttaga
    10561 tagttttata ttatatgtaa agaaagagct gaaactataa ctcctgatag caaattgcca
    10621 agaaatactc ccataaaagc atcttttatt ctcattttta atatggaagc tatagcagat
    10681 gcagtccatg ttcctgtagt tggcagtggt atacccacaa atagtattat accaattatc
    10741 gaaacacttt ttaatttctt cattcttgag tttattttcc tatcaattac atcagcaatc
    10801 cctttaaata gtttcttccc tcttaaaaat tgtaatatat gtctaaatgt caatatcaag
    10861 ggaatcgata ccaatgtaga acctattaca ctaaatacat aaacccatat aggatttaat
    10921 cccattgcaa tccctatagg aatggctccc cttaattctg ttacaggaac catagataaa
    10981 attaatatgt gtaaatatcc caaactattc cctcctattc taagtgacat taagttatga
    11041 atttatttgc tctataacct caccattctt tttactaact aaaaacgcat ttttagttaa
    11101 tgaaagcatt ggtaaatcac tcttagagtc tgaatatgca tatgaatttt cataatcaat
    11161 ctctatacca agcttaccta atacagcctt tattcttttt actttttcaa ttcccttaca
    11221 gttttctcca atgaattgat ttttatatct tgaattttca taaaaaaact cagtgcctat
    11281 aacctcatct gcaagaccat aatacttaaa atatttcata tatgcatatg gagatgcagt
    11341 tatcataatt ataacattat aaccttcttt atttttgtaa ataacctttt ttatagattc
    11401 attaattttt ttcattatgc aatcatcaaa aaaacttttt aaatcttctt cactaaaatc
    11461 tactattgga taaaagattg cctcttttgc tttcttcaac ggtaccattc taatcaaata
    11521 cataaataga ataaaaataa tatagggcaa ctttataata gttttaaaag ttttattttt
    11581 taaagagtaa ataaaaaaat ctatcatgct atcagtattt ataacagtat aatctacatc
    11641 aagtaataat acactttttt tcataagtat atccccatcc tatcactaaa aatatcacta
    11701 aaaaatcaat attattaaac atgtaattat ccacaagatt acgtttattt ttaaagcatt
    11761 atctgttagg ataatatcag taggattact ttcatttgta gtgttaatta tataattata
    11821 tctaagtaca ccataaacta caaatatggt tgtaaatatc atatgaggct tttctggatt
    11881 gcttgtagaa tatagtgcat aacatacaat tgtagaactt aatataacta tcatcatttg
    11941 atttagattt tctacgtcgt aatcttctag tatttttcta tgttcaactg catcacctct
    12001 taaagtttct atttcttttt tacgttttcc aagagctaag tacagagata agaaaaatgt
    12061 acatagaatt atccaatttg acaaactaac acttatggcc acacatcctg cataaaccct
    12121 aagtataaaa ccaagagata tggaaaacac atctaatagt attatattct ttagtttaag
    12181 agaatataat atattgttta tcaaataaag actgagaatt atagaaagat taaaactaac
    12241 aactgttgat aaacagactg aaattaaaga taatactaca aatagtatat aagcactttt
    12301 tttgcttatt cttccacttg ccactggtct attacatttt ttaggatgtt ttttatcctt
    12361 atccatgtct atagcatcat tcaacgcata tactgatgat gacattaggc aaaaacacac
    12421 aaatgcaata aacgtattta ataataaatt tatatttagt atgttgtttg aaaaaattat
    12481 agctgaaaat acaaagaaat tctttatata ttgctttatt ctcattaact ttatataatc
    12541 tgaaatttta gtaagaagcc ttatatttgt attggtctgt ttcataaact tactaccctt
    12601 tcaatattat tttttaaaca ggatatgatt ttatccctat gtttaaaatt tctataaatc
    12661 catttatatt actgattatt atagtaacca ataacaatga tagaaatata ttataaatat
    12721 ttttttcatt ttttctaaga gcttttgtaa agtaagccaa taagattata actgcaaaac
    12781 taaaatttat agccataata cttgaattga ctagattata tcctactata ccatgtaaag
    12841 ttagattgaa tataattatt cccatacata gcttaggaac tcttttttct gaattttcaa
    12901 taactgataa aacacaaaaa cataaagcca ataatgtaag aattactggt attgtagcaa
    12961 cttctccatt ttgcaaaaat aacccttttt taatacctgc tccaggaaaa taaaaaaccg
    13021 aatacaacag tctctctata atcattgcaa tcttttctaa aattggttct ccacttataa
    13081 atttcgtatc atactgtatt acatttccaa ggttcataaa actctcataa gacttcgttg
    13141 aaaccacaca aatatatgcc attaagataa atgtaagtaa atgttttaac atttttccta
    13201 ttttcaattt aaactcaaat atcatattaa aaaaatacaa atatatatta ctaatagtta
    13261 ttccaaatag aggaatagct gctaattcta ctaaataaga atttttccct ttcatcttgt
    13321 ttgcaataac tatgaaaaat attaatgaaa attgactata tatgaatctc tctacattta
    13381 aagtcataaa tagttgtgga aaagaaaata tcattatcat agtaagcaaa attaaagtta
    13441 gtctctgcat ttttattttt ttaatatgat taaaaactaa agttattgac atagcactag
    13501 ttattatttg tagacaaatt agtccaaacc cataagcact attaaaatct gttgtcccag
    13561 gatttgctat tgaagtacaa aataggtata ttggatatgc aaaaattgat ataattgctg
    13621 aaaataatat gtgcttacta ttatcttgac ttatagcaaa tacatttaaa ttgaataaaa
    13681 cccctgtatc tgtatctaac aatacatcat aggtattgtt taaatcataa aatatagtag
    13741 tcttagaaaa aaatatctta gttattatta agtaagttat tacaactaat ataaataaaa
    13801 atatctcgtt tttatatttt aatattttac tcatcaatat atcaccatcc attcattata
    13861 taggatttgc tgacacctac aatttattcc ttatatttaa tagccctctt cataatgttc
    13921 ttacttgttt tattgtaatt gaatagctct ttatttacaa ccacaatatt tgctttattg
    13981 atgttataat cagtcaaacg ttgtttatat aacatagatt agcgttgtat aaagtgagaa
    14041 tttgtaagca atattaatgt aaaatatttg aaaaatttta tataaaaata taaaattttg
    14101 cttatgtaat taaactataa caaattaaag cataatttaa ggagacaaaa ttataatctg
    14161 tctccttaaa cactaaaact atgttttaaa gaattactaa atactataca aatttcaaat
    14221 ataatataaa attaattaca ctttaacagt attagctcca tcttcttccc attttttaat
    14281 ctcttttagt ctatctatta taggtaaatg ctgagtacat tcaacctcac attttccaca
    14341 agatatacat tctttagctt ttgccttact attcattgta taattccaat gtcctaagcc
    14401 ataaactaac tttgtcattt cttcatcgct ctttttaaac atctgttttt cattgtaaaa
    14461 aagcatataa gctggggtat taataccttt tggacatact ttacaatacc cacagcctgt
    14521 acatatttcg ttcatattct tatttagtct agtctcaata gcatgtatat cctcatctga
    14581 ataaattcta ttttcgtctg caattttaca tgcatcatct atatcttgtt ttttattaaa
    14641 ccctatcaaa gatatactta tttgtgaaat tcctatgttg aatcttaaag ctgattctat
    14701 agtactttca tcatcttttg atagaaaaga aagctcttta ttatactttg gaatagttcc
    14761 accacctaat ggattcattg ccacaacacc atatccatta tcaacagcat actttgcacc
    14821 ttttaatcta tatggaaagt tcaatatatt aattcccata gttacacctt caaacttatt
    14881 ttcacttaga atatctataa cctcatctcc tggttgatga gatgaaaata ctatatggtc
    14941 tatcaatcct tcttctttac acttcaataa tccttcatat tgacctccag gtctcattgc
    15001 tagttcataa tgttccatct tcctaatatt ccaaacatga taaaaattta ttttagatac
    15061 gcctaatcgt tctattgatt tttttacagc ttctatagct ttttctgatg tatcacatgc
    15121 ttttggtttt tctttagttg atacataaaa atcggtctta ccttccttta tcatttgtct
    15181 aaaggctagt ccaaatatat cttcacttct gtcatcacaa taccctggtg ctgtatcaaa
    15241 gtaattaata cctttttcat acgcatattt gactaaatca gcattttctt gattactttt
    15301 ttctaaatca aaccttaatc ctccataacc aactactgaa accattttcc ccgtctttcc
    15361 atattcaacg tatctcattg taaattcctc cttagatata ttaagactta atttctttat
    15421 agaaaatatt ataccataaa taatttttat aaattattta tggtataata ttttttttat
    15481 aaattatatt tttttataac ttgcatttta tagctaaatt aaaaactata aaaaggttca
    15541 atatggacta ttacttgagc attctcatta atttcttcct gtatttgttt ctcaatttta
    15601 tgtgttaaat catgagattt ctctacactt ataaatgggt ctaccattac atgcatgtca
    15661 atataaatgg aacttttgct acctctactt ctgatattgt gaacatccct tacctcatta
    15721 aattcaaata caatttcttt tatataatcc tcatctacag ccactctatc taccaaaatt
    15781 ccaatagatg gcctaaatat tccataagct gaataaatta tgaatacaga aataataata
    15841 ggaactatag attctattat tactggaaat cctaacttga caccaaataa agtaaccaat
    15901 acgcctagag atacaaatat atcacttctt gtatgcaatg aatccgaaat caaaacataa
    15961 ctatttagtt tagtcccaac tctatattca tacatacata caaaaatatt tataaaaagt
    16021 gtaataatta aagcaattaa actttctata gttatagcag gtacaactgg attagctatt
    16081 cttgacactg cactaagaat tatcttaatt gctataacaa acaacatacc acttataaat
    16141 aaactggtta taatttcaaa ttttttgtgt ccataaggat gatttttatc ttttggtttg
    16201 gatgcaaaga aaatacctat aagccctact atattagatg ctccatccga caaagaatga
    16261 aatccgtctg ctgtcatact gtaactttta atttgattac caataatgat ttttaataat
    16321 gctactgcaa aattagcaaa taaaattatc cataatactt gcttcacttt cttatagtta
    16381 tccataacaa attccccctc ccttcgattc aataaaaaag ctatctatca atatcaatca
    16441 accagataat aaaatcgtat ataagcacat ttactatatt tatattcaaa attggttgta
    16501 cttgttcatt caatttgaaa cttaattata aataaaaata aacttctttt ttattattaa
    16561 aataataaaa tatattttat ttcatacaaa aaaagagtgc ctcatgaact aaaaagttca
    16621 ttttgagaca ctccattaga gttttgataa acaattaatt ttttatacta aaccttcttt
    16681 aactaagaat gaatgagcaa catcttctgg tgatttacca agttcatcca cttgatagtt
    16741 aagttctctc atagtttctt catttatttt tcctgaaagc atatttaaaa ctttttctaa
    16801 ttcaggatat ttcttcaaag tatcttctct cactaatgga gctgcataat atggtacaaa
    16861 gaagttttta tcgtcctcta atatcttaag attaaacttt ttaataagac catcagttaa
    16921 aaatgcatct ataacctgag attcattatt atctaaagct gtatatctta aacttccatt
    16981 cattgctttt acatctttga attttaaacc ataatctcta gaaagtccta caagaccatc
    17041 ctctctattt tcaaactcca aagttggtga gaatgtaaat tctttaccat atttagtcaa
    17101 atctgaaata gtatttattc catacttttc agcagtttca ggcatcatgg ctaatgtgta
    17161 agtattatta aatccaagag ggtcaagtaa agttaactga ttttcttttt ccatagtatc
    17221 ttttacaaca ttatatgctt catcagcatc tttaattgga gtatgtctca ttacatttac
    17281 caaaagtgta cctgtataat caacatacat gtctaactca ccagatttaa ttgcattaaa
    17341 tgcaacagat gaaccaccta gatttaaatt cttattaact ttaagattcg tatttttttc
    17401 tactaattca gcataaacat taccaagtat aagttgttct gtataatctt tagaaccaat
    17461 agttattgta tcttttttac ttgaaaaact agaactaaaa acaacgaaca acattgaaac
    17521 aacaactact acagatatta ctttaagtgc tatataattt ttcttaggtg cttttggatt
    17581 taatcctttt ggagtaactg caacctcaat tttgctaaat aaataatcaa ctaacagtgc
    17641 aattatacat gatggaattg cccctgctaa aatcatatta ttatttacag tttgaactcc
    17701 tgaaaatact aaatatccta aaccaccagc acctataaat gctgcaagag tcatcaaacc
    17761 aacagctgta actgcagata ttctaactcc acccattatt ataggaagtg ctaaagggaa
    17821 ttgtatttta aataatgtct gatttcttgt aagaccaata cccttagctg attccaatac
    17881 tactgggtca atacttgtta tacctatata cgtatttttt acaattggaa gtaatgaata
    17941 aactactacc ataaatatag ctggtttact tcctatacca agtataggta caagaagacc
    18001 caaaagtgcc atagatggaa ctgcttggac taagtttaca aatccaagta caagttttct
    18061 aagagatgag attcttgaaa taattattcc aagaggaaca ccaactatta tagctattaa
    18121 aatagatgta acagttagac tcatgtgttg tattaaaagc tctataatct tatctttttg
    18181 taatagtata aagttaaaaa aattagccaa gtacactcac ctccatttct aggaattgtt
    18241 cacttaaaac agaaagtaaa ctacttctag taataagccc taccagctta ttttcatcag
    18301 aaataactgg aatatatcca accgaattac gattcattac atttagtatt tctactaaat
    18361 tatcaccttc atttacatgc aaaggctcac ttgacataat atcagcaagt aatattgact
    18421 tatcatcaac atctttcata tcttttacag taacaatacc ttttaatgtt tttgctctat
    18481 ctataattag taagctgtca accttactcg ttctcataat ttctatccct tgagttacag
    18541 ttctagtgga attaacagca ataggatttt ttatcataat atctcttgct tttatatatt
    18601 ctggattatc ccaaactcta tcttctccga taaaatcttt tacaaagtcg tttgcaggtt
    18661 ttctaagtat attttcggga gtatcatatt gtgctattct tccatccttc ataatacaaa
    18721 ttttatcagc tatctttaaa gcctcatcca tatcatgagt tacaaatatt atagtttttt
    18781 tcaattcatc ttgaagagaa aataattgtt cttgtaaaga tgttctcgtt attgggtcta
    18841 aagcactaaa aggctcatcc atcaatatta tttcagcatc agtggcaata gctcttgcaa
    18901 caccgattct ttgttgctgt ccaccactaa gctctgaagg atacttattt aaaaattcac
    18961 cactatcaag tcctaccatc tcaagtagtt gcaatgttct tttttcaatt tcttcaacgg
    19021 atttttcctt ttttaggcgt ggaattagct ctatattttc ttttattgta agatgaggga
    19081 ataggcctgt gttctgtata acatatccta tttttcttct aagttctatt tcattttcct
    19141 gagaaattgg tttgccatta atatatatct caccagatgt aggttttatt aatttgttta
    19201 taagcttcaa ggtagtagtc ttcccacaac cacttgaacc aatcaaaact accaatgtac
    19261 ctgtttctac aactagagag atatcatcta atataacatt atttcctatt ttttttgtta
    19321 catttcttat ctctatcata atttatcatc ccctttttcg aacaagcaac cttcttcaat
    19381 attataagtt gtttttataa ttatatcata ctattttatt tatgtaaaca aagtacgact
    19441 tttagagcaa tatttgtctg aatattgaca caaatataaa gtatttatat atagtgtgta
    19501 atatataata tcgaaaagca aaaaatataa aaatattcat aaaataatac tagcttcaat
    19561 tatataatat taaaactagt attattttat ttaaacaaaa ctcacatttg gatactatcc
    19621 atgtattcaa ataaggtaat atctttataa gtttcttttt tatcacttaa atttgatatt
    19681 gtcactccca atagcctaat ttgttttcca ttcatctcaa catttctcaa taaattatct
    19741 atacctcttc ttatatcatt gtattcgtca atataatgtt ctaaactaag gcttctagtt
    19801 atctttgtaa agtcctcata tttaattttt aatgttaatg tttttccaaa cttctcagaa
    19861 ctttttaatc tatggcacac ctcttcacaa agttcatcaa gaatgttgcg tgtttcttct
    19921 tcatcaatat tcaaattatg acttaatgta gtctctgccc caacagattt tctaacccta
    19981 tttggttcta ctggtctatc gtctattcct ctagcaaatt tatataattc ataacctctt
    20041 tttttaaata tattttcaag ctcaaataaa tttaaacacc ttaaatcata gccagttttt
    20101 atacctaaat ttttaagagt atttgaagta acttttccaa caccaaaaaa tttattcaca
    20161 ggtaatttgt ctaaaaagtc ctgggcattt tcttcggtta ttacagttaa tccatttggc
    20221 ttttttaagt ctgatgcaat cttagcaaga aacttattgt atgatacacc agctgaagaa
    20281 gtgagaccaa cttctctaaa tatatctttt tttatttgct ttgctatctc aattgatgaa
    20341 tcaatatttt ttttattttt tgttacatct aagtaagctt catctaaaga taatggttca
    20401 actaaatctg tgtatctata aaatatatct cttatttgct ctgacacact cttataaaca
    20461 tcaaatctag gtcttacaaa aactgcatat ggacacctat tgtaagctac agctgatggc
    20521 atagctgaat ggataccaaa ctttctagct tcataagaac atgttgcaac cacacctctg
    20581 ctttgtggat ttccaccgac aataacaggt cttcctttta attttttatt atctctttgt
    20641 tctatagatg cataaaaagc atccatatct atatgaatta tttttctatg aagttccatc
    20701 atatcacctt taaagtataa atagattaat tttatttata ttacttttta attataccaa
    20761 acttatgttt ctttttggta ttttttcatt attttattta tatattcata ttttcaataa
    20821 ataaaaccac ctataatcca ttaacttcaa tataatttat aaaaaatcct taaaaacact
    20881 tgcaaaaact atataaaagc tttacaatag gtattccaag gaactaaatt taaaattcct
    20941 tatggataac agtatatata tttaatttaa atgggatgtt ttaaaaacaa aagactgtct
    21001 tatacaagac agcctactga ttttattata attgtaatgg actgctcaaa ctattacttt
    21061 aaaacagccc atattttttt atataatttt aactttaact atagtaaaga ttttgtgaag
    21121 gatactctgg gtcacaagtt acatctatac caagctttct aaatgtttgt tcatcatccc
    21181 tatttaatat agtagtagaa tgagcttgac acccttttaa taaggctaac ttttccatag
    21241 caaattgtgc agttggatta gttacagcac aaatactaag tgcagttaac acctcttgac
    21301 aactaagtga aacatttcta ctagctaaag tttttgtttt taagtttatg ataggctcta
    21361 atattacagg tgatattaag tgcatatcat cagatatatt agctaaatgt tttattgcat
    21421 ttaatactgc tgctgctgtt ccattcatta aatcagaacc cttaccagtt aatattgtac
    21481 catcttctaa ttctaatgca actacaggac aagtgtcatt cttgtcagca ctttctttca
    21541 atttattact gtaatttcta gcaggtaata caacatttct atcttctggc tttaagttaa
    21601 gttcttccat aatcattttc attctgtcat aagcaccttt atcaacttgt ccttttttat
    21661 actcacaagc agttttaaag tatcttctta ttatttcttc tatagaagct tttcttatta
    21721 cttcatcatc tattattcca aagccaactc tattaacacc catatcagtt ggagattgga
    21781 aaattgattc ttttccagtt atcttttcta ttattttttt aagtactgga aataattcta
    21841 aatctctatt gtagtttact gacatttcac catacgtttc aagatggaat gaatctatca
    21901 tattcacatc ttttaaatca actgtagctg cttcataagc tatattcaaa ggatgtttta
    21961 caggcacatt ccaaactggg aatgtctcaa acttagaata tccaactaca ttacctcttt
    22021 tatgttcatg atacaactga cttagacaag taccaagttt tccactatta ggtcctggtg
    22081 ctgtaactac tactattggt tttgtagttt ctatatattc atttgctcca tatccctcat
    22141 cactaactat agtctcaaca tctgacggat atccctttgt tggatagtgc ttataaactt
    22201 taattcctct acgttctaat ttatttataa atacagttgt agctggttgt ccttcaaatc
    22261 tagtaattac tacactattt acatctagtt cataacctct taaatcatct attaatctta
    22321 aaacttccat atcataagtg ataccaaagt ctcctcttat tttgtttctt tctatatctc
    22381 ctgcatagac acaaattaca acttcaactt tttctttcat atgttgtaat acttttatct
    22441 ttgcattttc atcaaatcct ggaagcactc tttttgcatg aaggtctccc atcaattttc
    22501 ctccaaactc taaataaagc ttgtcaaaat tatttactct ttctaagata tatttagact
    22561 gctcttctaa atattttttg tggtcaaatc ctactttcat cttaacacct cactaaatta
    22621 aatttcttgt tgataacagt atatttatat atattttttt agtaaaacat cttataaaaa
    22681 aatgttaata ctttttcact caaagtaata ttatacaatt aagattaatt tttttcaaga
    22741 tagttttatg gtaaataaga tatacttata taactaataa aataagaata ttatattttt
    22801 atatataagt ataatattct caccttagta ttaatttatt tcaatatttt catatatctt
    22861 ttactctaaa ttcaacactt tcctaatatc ttctatccct tgataaactt cttgtttaga
    22921 aaaattcata gcttctaatt cttcatcagt tttctccata agcatagtat aaaattctat
    22981 tgctatatat tgcatatttt cactatttac cgtcttagct ttatcaaata aaatttcttc
    23041 tgcttcgttt acttttccct gaaaaactaa tctttttaaa gttataagta taacttcttc
    23101 ttctgtatta tacactaaat ctttttcttc atattcactg tattttttcc ccaataaaag
    23161 tcttgcaacc ataagtttta tctcattgaa aatcttatcc actatagcca tgtttacatc
    23221 tcctataaat aaaatttatt ttctttttaa ttatatcaga ctttatagta aaaattatta
    23281 tatgtaaatg aaataataca aaaataaatt ttaacaataa aaaaagaagg tagcggggga
    23341 tttccgacta ccttcaaaca ctagattgtg attggtttgg tattttgttt atacccacca
    23401 aaagctttgt taaatcatct aaattgattt ttttagcatc cacatcctcc atcaaaaacg
    23461 tcaccacatt cacaaagtat taacaataat attaagaaag gtatccatgc acataagtcc
    23521 atttcagccc aacaatcttg gaatgctaag aataagaaga ataatattat tatccaccaa
    23581 gcacatccac caaatccgcc ttcaccaaaa aatctatcca acataatatg ttacctccta
    23641 gccattttgt atgtggagtt tcagctccta aaatatacta tgtttaaggg gtaatatttg
    23701 ctaatgattt tttaaaattt ttttagatat tttaaaaaaa attattgatt cacattcaac
    23761 gtcttaccat cactgttgat aactacctct ccattagaag tcaaaaatgt ttttatattc
    23821 aatttttcca gtatttttaa aatctcttca tctggttctt ttttctccga acaggtaata
    23881 acagcatatt taggtgaaat agattctaaa aatgcttcag ttaatttgtc atatcttcca
    23941 tggtggggta ttttaacaaa atcatatttt aaagtatttc catttataaa ctctgacaac
    24001 cttttttcct ctgcatctcc tgcaaataaa aacttatgtg ctccatagct tatactagta
    24061 attattgaat aatcatttga actttcgtac ttgcttttta aagctgattg tatgtttatt
    24121 tctgcactat taattacaat attcatattc tcttttagta gtactggttc tatatcagcc
    24181 tttttaagcg agtcaatata ttcatcatat tgcttactat ctttcttata atttgcctgt
    24241 ataatattct ttactttaac actagataac acactatcta ctccacctat atggtcttta
    24301 tctaaatgag ttaaaatcaa atagtcaagt gtatttattc ctttttcttt taatctatca
    24361 actatttgtt ttccattttt atcttctcca ctatcaatca ttatgacttt gtcacctatc
    24421 tcaactaata tacaatcaga ctttccagtg ttcagtatag tagtcttaaa ttcagtattt
    24481 tttgtgctac tctgaatatc attttgactc acactatttt tgtcactttt taaataagat
    24541 tttattgttg attttatctt accattagca gttatattta ctacaatcaa taatattact
    24601 attagagata atgtagtaac taaaaacagg atgtctttta atattatttt tttcatccaa
    24661 aagctcctat aaattttatt tattacatat ttatagtata gcaatcttaa tgttttaaaa
    24721 tacacatata aaaggggcaa attttaggca tatttgtggc atgataaaaa aatgactgta
    24781 tcaaaatgat tatctttcta aatttacacc ttcaaattct ttaatcttat cattagataa
    24841 aacactcttt ttagtagatt gtccattttt tctatcaaaa gtatttttag cttttacacc
    24901 tgataaaaac ttacctgctc cattaataca accttcttca cacatcattc cttctataaa
    24961 attaccttct aatcttccca atttagccat tgtcattgtc tttttaattt gtcttggacc
    25021 agatactttt ataggttcaa aatcaaaact aagccccttt tcagcaatat agttctgtat
    25081 tgatttagca agtccaccag atgctccaaa tccccttcca aaaatcgaac catcatttat
    25141 atcttcattt gaacattttg ataaatctat attaaatgct tcaaataatg ccattagttc
    25201 ttcaaaagta agtacataat ctactgaatc ttttaaacat ttctctgttg cctcactctt
    25261 tttagctgta catggtccta taaatacaac cttggcatca gaatctattt tttttactaa
    25321 tcttcctgtt gctaccatag gagacactgt agatgatatt ctatcttttt catctgggaa
    25381 tatcttctct atatacttaa aaaatcctgg acaacatgaa tttgtcatgt aattatctcc
    25441 tgattttatt ctttcaataa gctcattact ttcatgaact gtaactgcat ctgctccaca
    25501 agaagcttct atcatatcta taaatcccaa tgattttata gcatttttta actgaccata
    25561 tgtaatatta ctatctattt gacctgttat agctggagct actattgcat acatattttc
    25621 cttttttgca agtttttttg caacaggtgc tattaaactc ttatcagata ttgctccaaa
    25681 tggacatgct gacatacatg ctccacaatt tatacaatta tcttcatgta tcatggcttt
    25741 catagtgttt ctattaaaat ccaaagctcc tgttgggcaa acactcttac aaggtcttac
    25801 aacttctgat atagcatcat attggcatga acttttgcac attccacaag ccttacataa
    25861 atcatgattt atatatgctc tcccatttat ataggaaatt gccccaaatt tacatgcctc
    25921 cttacatcta tgagccaaac atcctctaca aagttctgta actgtaaatt catttattgg
    25981 acatctatca catgctgctt ctactatata tataatttgc tcgtctgtgt ccaaaccaac
    26041 tgaatctgga tttatatctc catatttact atttggagtt aagcttaatg caagttgcgc
    26101 cctttcaaga actacctctc tctctttata aacacagcat ctatatctcg ccttttctcc
    26161 tttaattatc tcatatggaa ttttttgtat attttctttt gttaaatttt cttcaataga
    26221 tagttttgct actgctgtca aaacttcatg tttcaatctt tttagttgcg tttcaaactc
    26281 aaacataagt aatcctccct ataaatcaca tctttttgct atatttctac tgttatgtat
    26341 attgtatatt ttatttattt ccataaatta ttataacata ggcatatact ttgttaagtt
    26401 atatacaaaa tattatatat gtaattttta ttttaagcgt atataatttt tattttagtt
    26461 gttcaataat taaatatata aataaaaata gagttgtcct aaagttaaac ttcaacttag
    26521 aacaactcta ttaacaatat gtatttttgt tgctttgtat tatctctatt tttctaaatt
    26581 tatattacta aaatctttta tctttgtatt tgctaagaca ctcttcttag ttgattgaga
    26641 attttttcta tcaaaagtgg ctttaacctt tgcacctgat aagaatttac cagaaccatt
    26701 tatacaacca ccttcacaca tcattccttc tataaaatta ccttctagct ttcctaaatt
    26761 agccatagtc atagttttct tgatttctct tgaaccagaa atctttacag gttcaaaatt
    26821 aacaccaata cctttttcag caagataatt ttctatagcc tttgtaagac caccagcagc
    26881 tccaaaacct cttccaaaaa ttgatgcatc atctatatct tcatttgaac acttagccaa
    26941 gtctatatca aatgcctcaa ataatgccat tatttcttca aaagtaagta cataatcaac
    27001 agctcctttt aattctttct tagctgcctc actctttttg gctgtacatg gtcctatgaa
    27061 tacaacttta gcatctggct catgactttt tataaatcta ccactagcta tcataggaga
    27121 tactgtagaa gatatttttc ctacatgatt tgggtatgtt ttttctatat agccaacaaa
    27181 tcctggacaa caagaatttg tcataaaatc ttctccattt tccattcttt cagcaaattc
    27241 taagctttcg tgaactgtaa ctgcatctgc tccacaagct gcttctaata catcaacaaa
    27301 tcctagtgat tttatagcat tttttatttg accatatgta acatttgctt caacttgacc
    27361 tgttatagct ggagctacta ctgcatacat tttctctttt tgaactaatt ttctagctac
    27421 aggagctatc aaactcttat cagatattgc tccaaatgga catgctgaca tacatgctcc
    27481 acagtttaaa catttctctt catgtatcat agccttcata ttttctctat caaaccctaa
    27541 ggcgttagtt gggcaaacac tcttacatgg tctaacaacc tctgagatgg catcatattg
    27601 acaagctttt ttacacattc cacaagcctt gcataattca tgatttatat aggccattcc
    27661 tccgacatgt gttatagctc caaatttaca agcttcttta catctatgtg ctagacatcc
    27721 tctacaaata tctgttactg taaattcatt tattggacaa ctgtcacatg cagcttccaa
    27781 aatatatata atttgttcat tttcttctat atccattgta tctaaatcta tcttatcttg
    27841 acctggttgt aatcccatac ttacatttac tctctcagat attatagctc tttctttata
    27901 aacacagcat ctgtatgtag gtttctcacc ttttattatt tcatactgaa tattatccat
    27961 tgcttctctg gtaaggttat cttcttttcc gagtctagct attgcagtta aaacatcatg
    28021 tcttaatttg tgtatttgtg tttcaaattg aaacatttta ataccacccc cgtaaatgat
    28081 tttatacgat accattataa cacaatattt ccttttatta atatatacat aaaatattat
    28141 atgtttaaat tttattttat cgaatatata tagtattttt tcatcttatt atataatatt
    28201 attgatattt ctgaaatttc actattttta tttttacttt tcaatcttat atataatatt
    28261 ttttgttata tattttaatt ttacttttta cttacatacc tttttattac attttttgat
    28321 attaaattta ttatttgtat ttacatttcc gtcaaatttt gtaatacttc tcactatata
    28381 atttaacaat ttttattaaa ttctaaaagt aattaatttt tttaaaaatt ttatttgctt
    28441 ttatttaaat tttatgttat attaatagaa cagacttatt tttttagttt gtatttttta
    28501 ataatgcgga ggttttctta taatgaaaaa cggaatagta aaatggttta acaatgaaaa
    28561 aggatttggg ttcatatctg tagagggaga agatgatgtt ttcgtacatt tctcagctat
    28621 acaaaatgat ggatacaaaa ctttagaaga aggcgaaaag gttagctttg atataactca
    28681 aggtaataga ggacctcaag ctgaaaacgt aaatagaata taattattta atttaaatat
    28741 atatatttta gaaaaggagc tatcgagggc tcttttttgt tgtttattat atttattttt
    28801 aattacaaaa ataagaagtt actttagaaa aacgttaaac ttaaactaat ttttttaaaa
    28861 taacttctta tattataata catattattc atataaaata ctctatttaa ttttctcaga
    28921 tattatcaag cataactctc ttaagatttt acaggctata actgtcgata cacctgtagt
    28981 atcataatca ggacttaact ctacaatatc acaaccaact atatttatat tagaattttt
    29041 tataatctta aaaatctctt gaaactctct atagtttaca ccaccaggtt ctggtgtacc
    29101 tgttcctgga aatacagatg catccaatac atctaaatct atagtaatat aaatattctt
    29161 tccatttagc atattaacta tcttttcaaa tgtatcaatt ccgcctattt ccatgtatgt
    29221 gtgtttttct tctgtagcaa atttaaattc ttcttttgtc ccagacctta taccaaattg
    29281 aaatatttta ttatcaccta caatatccca tattctttta attactgttg catgagaatt
    29341 tttgctatta ttatattctt ctctcaaatc cgtatgggca tcaaaatgaa ttacatatat
    29401 atcattgtac ttttcatata ctgctttaaa ggctggtaat gtgactaaat gctctcctcc
    29461 aatcataaat ggcacttttg aatctctaac aatcttatat gtttcttgat agatttcctt
    29521 taagacttgt tctgtacttc caacactaat ttctaagtct ccataatcac atatattata
    29581 atcctctaag tccaaatcta aaaaaggact atacgtctca agaccgtaaa attcttttct
    29641 catagagcta cttgcaaatc tagctccagg tctattagaa gtagttccat caaatccaac
    29701 accaaatact ataagattag attcttcata atttttgtcc atactcataa aggtattcat
    29761 atgatagaaa ttattcttca tctagcatct cctttacata ttgaggtaac ataaacgaac
    29821 ctaaatgtat atcactatta taatatttag tttttaaact taatttctcc cacccatttc
    29881 tatcttgatt ttttactgga ttaagttttt ttgaagcaaa tccaaacaac cagtgaccag
    29941 atgggtatgt tggtatatgt gcttgataga cttctgatat tggaaatatt tgttttaact
    30001 ttttattagc tcttttcatc tctttagcat taaagtcaaa atacggactt tcactttgat
    30061 ttactaaaat tccatcatca gtcagtattc tataacaatc cttataaaaa tcagttgaga
    30121 aaagtccttc gcctggtcct attgggtcag ttgaatctac tattattaag tcatatgatt
    30181 tatcatgtgc acatttaaca aaattaacgc catcttcaaa atataaattc actctttcat
    30241 catctaattt acatgagcat atatccatat acttcttaga tacatctaca accattttat
    30301 ctatttcaac catatcaatc ttttctattt gaggataacg gcttaattct cttactgtac
    30361 ctccatcacc tccacctatg actaatactt tctttatgtt taaatttgta gccataggaa
    30421 catgagttat catctcatga tatataaatt catctttata tgttaccatc attaacccat
    30481 ctattgtcag aaattttcca aattcatcac tatcaaatac gtctatcctc tgaaattgag
    30541 attttccttc aaatagatgt ttatttactt ttatagaaaa tcttacattt tctgtccatt
    30601 cttctgtata ccaaagttcc atataataat cctcactttc tcttgtatgt tttatactgc
    30661 ttctggttta tgagtaatct tacctaaatc tttttttgaa tattttttga tttttgtagt
    30721 taaacctctt ggaatttctg tagactcgct tctttcagat tttaaaagtt gctctaataa
    30781 ttcaaaactt ttccatgggt ctatatcacc acaagtaaaa aaatctgctg ctgcataacc
    30841 atattcaggc catgtatgta ttgtcaaatg agattcagca attataacag ctcctgaaac
    30901 cccccaagga ttaaaatggt ggaatacaga atcaacaatt gtcgcctttg catttagtgc
    30961 tgaatcattc ataaaatttt ctataagacg tgggtcttta agtacgtttt catcacaatt
    31021 atagtattct actagtatat gtcttcctag tgtttcaaat ttcatttcaa ccccaactcc
    31081 tttatacttt taataagttc tatctgataa aatctattta acaaacactt gcccttgcaa
    31141 aataaaataa tttattttat tactagtatt ctatctaagt ttttatcttg tacatctgta
    31201 agatatgcat tgctattttt taagaaaata atatactcca taatttcttc tgttatcaat
    31261 tctcctggag caataatcgg tatacctggt ggatatgcca ttattgattc tccacaaatt
    31321 ctatctatac atgaattaat ttctacactt tcctttggtg cataaaaagc ttctcttgga
    31381 ttaagtttaa taattggatt tatttgaacc atattaaatt catttaatgt tgattctttt
    31441 ctataaatct ttgcaattat agaaagtgct tttactaatc tatctatatc tgatttattt
    31501 gttccaatag acacaagtgc cagaatatta tatgaatctc ctaattctac ttgaattgaa
    31561 aaatttttat ataataaatc gtatacttca aatccagtca aatttaattc ttttacattt
    31621 atacacaatt ttgtttcatc tataaattta acaccttttt gttttaatat ttctgtagat
    31681 aaaactttta ttccatcaat tttgttcaat ttagattttg catacctaga taggtttaat
    31741 gcttttgaca gttgcttttc accattctca actagattaa ctctagcacc atctatactt
    31801 gccataagca aataagatgc tgatgttgat tgtagcatat tgattgattg aagaaccttt
    31861 tcaaagctta cattatctct attcaataag agtgctgaag attgtgtcaa tgctcctcca
    31921 gttttatgta tacttacagc taccatgtca gctcccaaac ttattgcaga aggaggtaaa
    31981 tctaaatgaa atggaaaatg tgcaccatga gcttcatcaa ctaacactaa aacattattt
    32041 ttatggcata gttctataat actctcaagg tctgcacaaa ctccatagta agttggatta
    32101 aggaaaaata atgcttttat attacaatct ttttctatct cttttctaac tttttcaaaa
    32161 gtaatattta aacttatacc taatttttca tcaaattctg gttgtataaa tattgggatt
    32221 cctccacaaa gtattaatgc atttatcact gatttatgta tatttcttgg aagtaatact
    32281 ttttcacctg gttttataac actcaaaatc attgcatgta ttgcttgagt tgtgccattc
    32341 gttataaaga atgcctcatc agccatatat gcttgtgcta gcaaatcctg tgcatttttt
    32401 ataatcccat ttggggcaga tacattatcc atagttggtg atgaatttat atccattttc
    32461 atgagacttt cacctagata tttatttaat atagctacac ctctattttt tacatgtcca
    32521 ggtacatcaa aacaagctat gtctttctct gagtattcct ttaacgactc taataatgga
    32581 gcacactcat agcttaattc aactgcattt gcaaactcgc tatccatgat ttttaaattc
    32641 atctcctctc tatattcaaa catttttaat atttattatt tattatttct attttttatt
    32701 tttactttta ttaaactcat gatatttatt atattaacat tccagcattt ttacaagtgt
    32761 ttttttgaag ttaatcaaaa acacatatat cag
//
LOCUS       DAMJVM010000038        32347 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.038, whole genome shotgun sequence.
ACCESSION   DAMJVM010000038 DAMJVM010000000
VERSION     DAMJVM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32347
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.038"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            349..1110
                     /locus_tag="PC523_001861"
     CDS             349..1110
                     /locus_tag="PC523_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2475386.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFIGVSIAVGMFLVLGLYFKLGGI"
     gene            1111..1605
                     /locus_tag="PC523_001862"
     CDS             1111..1605
                     /locus_tag="PC523_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2475387.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTRIKENEKYKNSINKI
                     KQQK"
     gene            1829..3007
                     /locus_tag="PC523_001863"
     CDS             1829..3007
                     /locus_tag="PC523_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475388.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVGAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            3263..4870
                     /locus_tag="PC523_001864"
     CDS             3263..4870
                     /locus_tag="PC523_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HDF2475389.1"
                     /translation="MKKERLQHEDLKEYDYDEDVYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGIACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESS
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPVYDEEGKDKWK"
     gene            4861..5565
                     /locus_tag="PC523_001865"
     CDS             4861..5565
                     /locus_tag="PC523_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2475390.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQGNINNDAPKSFEVKNLKIDYENHTVLINDEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(5854..6012)
                     /locus_tag="PC523_001866"
     CDS             complement(5854..6012)
                     /locus_tag="PC523_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475391.1"
                     /translation="MKIFSFMLYVVGICFCIAGFIGHYPAFAGATLSFFATTMIAVNQ
                     GKTKKKMK"
     gene            6717..9815
                     /locus_tag="PC523_001867"
     CDS             6717..9815
                     /locus_tag="PC523_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2475392.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKALRINDISNMK
                     CYEAIQGRDSPCEFCTNSILKEDGYYVWENTNTLINRHFILKDKLIKWEGKLARIEIA
                     LDITEKENISKSISEKLEIKELLVGCIKHLITESSFSDAVDLVLSSIGKFHKADRAYV
                     FEVSEDRKKVINTYEWCNEGVDEQKQLLTNLDIKELKNWFLLFKQKGFIIIDDVEDIK
                     EEHPMEYEILKPQKIKSLMASSLKEDDKIIGFIGVDNPRKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTSAYNRNRYIKYIDTFQKDKVKSIGVVFIDINGLKDIND
                     NFGHASGDAIIMSVSVMIQKYFHSYYVYRIGGDEFVVLCEDISKQLFEELVSWLTEEL
                     KDNMECSAAVGSIWSEDDIDIHKLIKVADERMYQDKKRYYNKIELRNNEMKVVSDLSD
                     STNNISLKCRDLDNEVYFDDLMIKGYSKAYEKLAYNTYDIILSICMDTNLITPIYQSE
                     SNILNFPIKENIEKIFSYIKKHLIHPDDIILFDDNLLLKSIDSPNNSKQGDLELNFEY
                     RHLGIDEKYHWALLYILKISSKNNLGEKQTHILVAIKNIDYLMQIHKNQKDTLTGLYN
                     YESFCEQTSLMLKRYPGKRYAIIMMDIDKFKVINDIYGIRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDREIHKVIYNIKNNLNRKKFEYMLVPCFGVYKILDNH
                     IPITSACEMAGYAHKKVKKQSINHYIFYDDTIRNDALEEKHMEREMEYALENGQFHVY
                     LQPKYNLNTNKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIKLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYGIPKSFLELELTESLFIKN
                     IKLFTKVIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNGEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSDDFEKLLY
                     QK"
     gene            9938..11224
                     /locus_tag="PC523_001868"
     CDS             9938..11224
                     /locus_tag="PC523_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2475393.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDNDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFKLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRYSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAISENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(11484..12590)
                     /locus_tag="PC523_001869"
     CDS             complement(11484..12590)
                     /locus_tag="PC523_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2475394.1"
                     /translation="MLDKNRLINNFIDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPVLDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPENLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGKIEGGGATNIVTDRVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHNMSYPSFELSRDSHVFKLSEEAIRQVGIVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(12625..14166)
                     /locus_tag="PC523_001870"
     CDS             complement(12625..14166)
                     /locus_tag="PC523_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2475395.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SVVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAKLDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMIFNIPLGPGIL
                     PSYSM"
     gene            complement(14721..16031)
                     /locus_tag="PC523_001871"
     CDS             complement(14721..16031)
                     /locus_tag="PC523_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2475396.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYNNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKEGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHSSDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVKITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIVPAK
                     AMALTVVDLLFDNAKNAKDILDAFTPVMTKEEYLTFLEKHDKTL"
     gene            complement(16044..16514)
                     /locus_tag="PC523_001872"
     CDS             complement(16044..16514)
                     /locus_tag="PC523_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475397.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            complement(16518..17369)
                     /locus_tag="PC523_001873"
     CDS             complement(16518..17369)
                     /locus_tag="PC523_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HDF2475398.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVIGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKNTKEI
                     KSTKGEC"
     gene            18096..19007
                     /locus_tag="PC523_001874"
     CDS             18096..19007
                     /locus_tag="PC523_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF2475399.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMMKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNIINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKEEVFSRKVIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDNTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLKELSKIYSL"
     gene            19164..19661
                     /locus_tag="PC523_001875"
     CDS             19164..19661
                     /locus_tag="PC523_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDF2475400.1"
                     /translation="MNYEIVEISEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKSDKTIGLYTDYQGDFTSPYNFVACCEVNSNSDKDKNLEELNTNNTVGEIII
                     SKVIPAGKYAKFVIAGGQKEVGDFWLEFWQMDFDRTYVSDFEEYQSNTFDTNKHEIHI
                     YIGIK"
     gene            20396..20758
                     /locus_tag="PC523_001876"
     CDS             20396..20758
                     /locus_tag="PC523_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2475401.1"
                     /translation="MGNKILIVDDAIFMRMIIKEALGKSGYNNLVEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVDAIKLGALDFI
                     VKPFKTETLVKVVNNALK"
     gene            20823..21440
                     /locus_tag="PC523_001877"
     CDS             20823..21440
                     /locus_tag="PC523_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HDF2475402.1"
                     /translation="MINYLELDDAHIDALREIGNIGSGNAITSLASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEVP
                     LDDIEEIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFVENKFKMSDNYFTSHILMIPEMSSLREILVKLGLEI"
     gene            21437..21922
                     /locus_tag="PC523_001878"
     CDS             21437..21922
                     /locus_tag="PC523_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HDF2475403.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKYKFADTGIKELIKSMENIGANRIYIKAKIAGGAQMFLGNSN
                     SAIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELIVDSINKGKKK
                     L"
     gene            21965..22483
                     /locus_tag="PC523_001879"
     CDS             21965..22483
                     /locus_tag="PC523_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HDF2475404.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMITNCMYIVVTEIKDLVVGFV
                     VDRVDDIISLEKSEISPPPRVTIEYSDYFVSGIGELDGKVITLLDLEKLLTDKDEILI
                     DELINGFDCTIM"
     gene            22619..23578
                     /locus_tag="PC523_001880"
     CDS             22619..23578
                     /locus_tag="PC523_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2475405.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKALTKDNFKVKKAFNAIEAKK
                     FIEEFIPDIILLDIILPDNSGFDFCKEVKSNARTADIPIIFITSKNADTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTIISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEDAKILSERIRKEIKEFDFNYKENKIKCTITLG
                     VVEVNSNISLEENISLADKVMYEGKNLGKDCSVVSTMKGLKKI"
     gene            23604..25307
                     /locus_tag="PC523_001881"
     CDS             23604..25307
                     /locus_tag="PC523_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDF2475406.1"
                     /translation="MKREVKLSKLAIVLLVIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQNIIFSSDYINSQEKMDSLI
                     NEFQEDIVARSNAKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVDFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVKIAKETANALELVVNGVEKTTDYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKEREVRESSW
                     V"
     gene            25478..27580
                     /locus_tag="PC523_001882"
     CDS             25478..27580
                     /locus_tag="PC523_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDF2475407.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEDYVSTLELSSSMKSKDNASLESTDEIEVIDLNFAYREG
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFLDFYPKDVETNAESTEYIKNYGFYIQ
                     FVAKEDNSIVYKIIEENLYVKEYINISKKEIEMKSTSNNNEVENKLKDSNKVSKSYKN
                     NYTEDELQEDVLNEKTDIEKDGLSGENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKSTRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLTEPFMHLIRNAMDHAIEPIEERIAKGKKEVG
                     KIVLSAENLGGEAVISISDDGRGIMKDKILAKAKEQGLLKHQTNEYTDEEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKPDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEENKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMHGNMIIIENENKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            27593..28033
                     /locus_tag="PC523_001883"
     CDS             27593..28033
                     /locus_tag="PC523_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDF2475408.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPEADYNERTCIIITKVDEYQVGIIVDRVIDVIPI
                     ENLNLLATTNSNNININKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            28069..28872
                     /locus_tag="PC523_001884"
     CDS             28069..28872
                     /locus_tag="PC523_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDF2475409.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIERKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSIFPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSNTKYKYVKPAIYKR"
     gene            28912..29508
                     /locus_tag="PC523_001885"
     CDS             28912..29508
                     /locus_tag="PC523_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HDF2475410.1"
                     /translation="MGRFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGCQMKLCADKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGANGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            29614..30471
                     /locus_tag="PC523_001886"
     CDS             29614..30471
                     /locus_tag="PC523_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HDF2475411.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLKNASIN
                     LNNKEAVSVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSNKI
                     VERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSIKV
                     RYYTYSGGAHGFYQDTAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELEKK
                     DEENIGIYNFKGIKENQNFYLQDDNLVIYFDLYDITPYAAGIPEFSINKKLISSMLKP
                     EYVDLFDLK"
     gene            30539..31528
                     /locus_tag="PC523_001887"
     CDS             30539..31528
                     /locus_tag="PC523_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDF2475412.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNIPV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGVGGQGEPQLEAMIEARNLDEVRVFSR
                     NKENREKFVEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVFNGNLVKKG
                     ALISAVGSYMPDMQELCPICLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGMDWKW"
     gene            31620..32231
                     /locus_tag="PC523_001888"
     CDS             31620..32231
                     /locus_tag="PC523_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475413.1"
                     /translation="MKMVKKLILSSIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGIADALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANNLVKSYVTDEKNYDKKEKEVQDDMAK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT        12551 a         3619 c         5568 g        10609 t
ORIGIN      
        1 aaattatatt ttgtatcata aaaataatat tttatattgt acatatataa atatgaagtt
       61 gctgtaaact taaagagcca agagaaagaa taaaaagaga tataatttta tttgtagtaa
      121 aaagttatac aatttagaag gttatataaa tggttgaaag tgtttgtcaa atattaattc
      181 agccatttta tggtgtaaac aaccttaaaa tttggttttg acaagcataa aaaacaagtt
      241 ttttcataaa atgtaaatat tggatattat ttatttgaaa tagtttaaat aaataataac
      301 ttgtattaaa atatgaataa tactataaaa gaatgggaga tatgttttgt ggatatagat
      361 agaattttaa acttttcctc aaatgcaggt aaagctatgt tgcaaagcgg aggagaaacc
      421 tatagagttg aagaaacgat agctagaata tgtcaatctt ttggaataga tcatgtggac
      481 gtatttgcta ctccaacagc tgtgatggca tctgtatttg tagatggaaa attacattca
      541 gcaattaaaa gaataagctc aagaagagta gacctaaact tggttcatga agttaactca
      601 ttatcaagag caataagtat aaataattta gatattgaat tatgtgagaa aattttggat
      661 gaaataagtg aagataacta ttactcagat ttaataacaa tattttttgc aggtattgca
      721 gcagcaactt tttcgatatt atttggagga aatgtggaag aatttatagc tgcatttact
      781 gttgggattt tgactaagtt tgttattatg tacttaagta aatcttccct taatgacttt
      841 tttgtaaatg caattggagc agttattata gcttcttgtt caattttgat tttaaagctt
      901 ggatttataa agacgctaga ccatctaata gcaggagcaa taatgctttt agttcctggg
      961 cttgctttga ctaatgcact aagagatttg ttagatggac agttgatttc tggtcttgca
     1021 aaattagcag aagtattttt tataggtgta tctatagcag taggaatgtt cttggtactt
     1081 ggattgtact tcaagttagg ggggatataa gtgcatttgt ttatagaagt aatagcagct
     1141 ttttttactg ccttaagctt tggagtgcta tttaatatga aaggaaaaaa cttaatatta
     1201 gcaggaatag gtggaagtat agcttggttt tcatataaat ttcttttaaa tatgggagta
     1261 acagaaaact tatgtttttt tatagctaca gtttgttttg cgatatattg tgagttatgt
     1321 gcaagaattt atatgactcc agcaactaca ttaagtgttt gttgtttaat aatattagta
     1381 cctggttatg gaatatataa tactatgtat tctgttttaa ccaataatta tataaaagct
     1441 gtagaatatg gtgtgagtac attatcatgt gcaagctcaa ttgcattagg tttggtattt
     1501 ataactactg tatttagaaa agtcaactta tatggagtgc ttacaaggat aaaagaaaat
     1561 gaaaaatata aaaatagtat aaataaaatt aaacaacaaa aatagaaaaa attgactttt
     1621 aaacaaaagg atgataatat aataacataa aatagaataa gagtatccaa aataaagatg
     1681 atattcttat tctatgaacg taattttttt atttcaaaaa actgacaact tgcattacga
     1741 gtgtaagttt tagaactcag tttgtaccaa aaagtttgta ccaatgttag gaaccttaat
     1801 ttaaaattaa aagggggaag atttcaaaat ggaaaagctg tcggataaga gaagagtcaa
     1861 ggcagtaagt atggaaacct tcgtatttat tgtactattg gcggtaggat ttggatatgt
     1921 aggaagcatc atgggtgcag gaatgatgtt taaagtaata atgagtacag cacatgcgct
     1981 tttattagac acagtatttt taataatggc tatggcagtt cttgcaggtg cacttagtgc
     2041 gttgctttca gagtttggag taatatcttt agttaataag atatttaagg ggcttatgag
     2101 acctatatgg ggcttacctg gtgctagtat agcaggagta gttgcaacat atttatctga
     2161 taatccagct attataccat ttgctaaaga caaaacattt acacaatatt ttaagaagta
     2221 tcaagtacct gctttatgta atttaggaac agcctttggt atgggactta ttgtaacaac
     2281 ttttatgata gctcaaggta aagaatatgt actaccagct ataataggta atgttggcgc
     2341 aataataggt agtattatta gtgttagaat tatgttgact tttactaaaa agtattataa
     2401 ttatgaccct aaaaatgata ctgaaaagca aataaatgat aaaggtgcaa aacttgaaga
     2461 gtttagagaa ataagagatg gtaatgtatt ccaaagaaca ttggatgcta tacttgaagg
     2521 tggtaagtta ggtgttgaaa tgggtatggc tataatacca ggcgttttag tagtttgtac
     2581 attagttatg ttattaactt ttggaccatc tacagaccct gctacaggtc aggctgttta
     2641 cacaggagct gcatatgaag gtataaaatt attacctgct attggtgata agattagctt
     2701 tataatagag ccattatttg gatttacatc accagaagca atagcattcc ctgtaactgc
     2761 attaggagct gttggagcag caatatcttt agtaccagag tttataaaat caggcgctat
     2821 aactcctaat gatatagcag tattcacagc aatgggtatg tgttggagtg gatatttaag
     2881 tacacacata ggtatgatgg atgctctaga tgcaagacca ttagctggta aagcgatact
     2941 ttcgcatact ataggtggtt tatgtgcagg tatatgtgcc cactttatat ttatgctcgt
     3001 tggataagaa tattcgataa atcaagtatt ataatttatg aaattaaata aaactaataa
     3061 tttaagtata taaaaattaa attatcaatt taaatatata gaggtgctgt tttgaaatag
     3121 agatttattt tcattttaag acagcacctt ttagtaattt aaatacatta attaaatgta
     3181 ttgattgaat ttattatgat aaaattgtat tatataaaat agagccaatt taaacagtaa
     3241 caataaggag gaattgtagt aaatgaaaaa ggaacgacta caacatgaag acttaaaaga
     3301 gtatgattat gatgaagatg tttatctcag taaaaagata attattaagg acttcataaa
     3361 aactatatta gttatgacaa ttgctactgg aatatcagta gtttttgaaa aagtaggttt
     3421 taatgaatct aatattatac tagtatatat attaggtgta ctatttgcta caagtataac
     3481 ctatggatat ttttttggaa tactttcttc agtaatagga gtgctatcat ttaatttttt
     3541 ctttacacat ccattatata cttttttagc ttatagaaaa gattacccta tgaccttttg
     3601 tattatgctg atggcagcaa ttataacaag tactttaact tctaggataa aaagagaagt
     3661 gagactttct cataagagag aaaaaatgat aagattactt tatgagaata acaaaacgtt
     3721 gcttaaagca aaaaataaaa atcaaattat tgaattttgt gggtctaatt tagtaaatat
     3781 attaaataga actgtaatag tgactattgc agatgaaaat aataatctag gtaagccaag
     3841 tatttatatt tgtaataatg atggaagtga gaatatattc cattcagtat tagaaaagga
     3901 agctataaaa gaagctttta ggacgggtaa cccagttgga attgggacaa aatattgtaa
     3961 aaacagcagt gcatattatt ttcctataaa aggtcaagaa tcaaatgttc ttggtgttat
     4021 aggtattgcg tgttttaaga tagatattat tacagaaaat gaaaagatac tattagaagc
     4081 agtttcaaca caggttgcgt tggcaattga tagagaaaat ttatttgaaa aaaataaaaa
     4141 agcaaatctt gaggcagaga gagagagatt aagaggaaac ttgcttaggt caatttctca
     4201 tgatttaaga actccactta caagtatatt gggttcatct tcaactatac ttgaaaatga
     4261 tgatgtgata gataaagaaa ctagagtgga attattaaaa aatatatatg aagatacgag
     4321 ttggctaact cattctgttg agaatatact tagtatgacc agaatagatg aaggaaaatt
     4381 ggatattgaa aaacgaccag aggttgttga tgaaataata gcagagtcta tattaagagt
     4441 caaaaagttt gccaatagta gagatataaa aaccaatatt ccagatgaga taataatcgt
     4501 gcatgtggat gtattattga tagaacaagt tttagtaaac ctaattgata atgctataag
     4561 gcatacacct aaaaattcta agatagaact aactgtaaaa aaagagtcaa gtcaagtgct
     4621 atttgaagtt gcagataatg gaaaaggaat accaaatgaa gatattggta atatttttaa
     4681 taggttttat actaagaata agtctagaaa tcttgaaaga agaggtattg gtcttggatt
     4741 ggagatatgt aaatctatag tagaagctca tggtggagaa attgtcgcct ttaataatcc
     4801 ttctggagga gctacattta gatttagcat acctgtgtat gatgaggagg gaaaagacaa
     4861 gtggaaataa agccattagt tttaattgta gaagatgata agccaatttg taaatttata
     4921 aaagtatctc ttgaaactca aaattatagg tgtgtagaga cagataatgg aggaacagct
     4981 atatcactta tacattctct tgacccagat ttgataattt tggatttagg actaccagat
     5041 attgatggaa ttgaggtaat aggacgagtt agggcatgtg ccaagactaa taaaataatt
     5101 gttgtttcag caagggagca tgagagggat aaggtggagg ctttagatgg aggggcagat
     5161 gattatttaa ctaaaccttt tagtgtaaca gagcttttgg ctagagtacg tgtagctctt
     5221 agaaataaag ctcaacaagg caatataaat aatgatgctc caaaatcttt tgaagtcaaa
     5281 aacctaaaga tagattatga aaatcatact gtgttaatta atgatgaaga aatacattta
     5341 actccaattg aatataagat tattgagttg atgtctaagt attcaggaag agttttaact
     5401 cataagttca taatagataa agtttgggga aattactatg aaagtgaaaa tcagtcactt
     5461 agagttttta tggcaagtat aagaagaaaa attgaaaaga atcctgctca gccagaatat
     5521 attttgactg aggttggggt aggttaccgt atggcagatg aatagatact aaatgggtag
     5581 ttgatgagtg gtcaccaaat gaatatgagt agtaatcaga tagaaattat ttgttatata
     5641 agaaaatata agtattttaa atcgaataca aatagctatg tatattttat aatgaaatgg
     5701 atagagagtt agtctatcta atattgaatt tacatagcta tttttatttt tccaagagaa
     5761 tgtaaagttg atgatgtatt tctataatag aaaaagtaag ccaattttag actaactact
     5821 tgtgtaatac tagcttgtaa agttggcatt tacttatttc atttttttct tagttttgcc
     5881 ttggtttacg gctatcattg tagttgcaaa aaaactcaat gttgcaccag caaatgctgg
     5941 gtaatgtcct ataaatccag caatacaaaa acatattccc accacataca acatgaaact
     6001 aaatattttc ataactattc tccctttaaa atttaattta ttaagcatat tttatcatat
     6061 attaatagtg aaatcatcag ttttgatgac gtgaaaaagt atgtcatatt ttatggttta
     6121 tcgtgtattc tttgtatgat tacttatcag accatatttt ttaaggtgtg ctaagtgtta
     6181 gtttgtaata agctctcgaa aactatatgc taataagtgt aagaaaagta agtagaattg
     6241 ttagatataa tatttatatg gataaatcaa tttaactctt ttttgatata aaaaaattgc
     6301 cctttttatc tttttatgtc agcctttgtg atattatata atatataaaa gtaacaaact
     6361 aaataactta ttaatattga gaaatcttaa tatataatag ttttatattt ttaaatgaat
     6421 gaagtattat ctttctatat gattggaata cttaattgaa gtggagattt attatttata
     6481 tgatgattaa ttgtaaatac acaatacgat aattaattat agatatatta aattaagtat
     6541 tacaaagctc tactgtacaa ttgacacgat aataactcta ttgactaaaa aatagaactt
     6601 tattaaggtg ctattttaaa atataaagaa gcataacatc attgctaaat acatagcaaa
     6661 attttaaaag atttaaaatt caatctggga aaatgaataa aaggagatat atataaatgg
     6721 atgtaataaa tttaatagat gaattggatg aaattgtata tgtttcttct ttagatacat
     6781 atgaactttt atatattaat gaaattggga aaaaggcact gaggattaat gatatatcta
     6841 atatgaaatg ttatgaagca atacaaggta gagactctcc ttgcgaattt tgtacaaatt
     6901 ctattttaaa agaagatgga tattacgtat gggaaaatac aaatactcta ataaatagac
     6961 actttatatt aaaggacaag ttaattaaat gggaaggtaa acttgcaagg atagaaatag
     7021 ctcttgatat tacagaaaag gaaaatataa gtaaatcaat atcagaaaag ttagagataa
     7081 aggagctatt agttggatgt attaaacatc ttataacaga atctagtttt tctgatgctg
     7141 tagaccttgt attaagcagt attggtaaat ttcataaagc tgatagagca tatgtttttg
     7201 aagtatcaga agatagaaaa aaagtaatta atacatatga atggtgtaat gaaggtgttg
     7261 atgaacaaaa acagttacta acaaatcttg atattaagga attaaaaaat tggtttttat
     7321 tatttaagca aaaaggattt ataataattg acgatgtaga agatataaaa gaagagcatc
     7381 caatggaata tgaaatttta aaacctcaaa aaatcaaaag tttaatggcg tcttcattaa
     7441 aagaagatga taaaataata ggatttattg gagtcgataa tccacgtaag gctgttggag
     7501 acacctctct tttgacatcc ctatcttatt ttttattaac tgaaatgaaa aaaagaaaag
     7561 tagaaaataa attaaattat atgagctact atgatttctt aactagtgca tataatagaa
     7621 atagatatat taagtatata gatacttttc aaaaagacaa agttaaatct ataggagttg
     7681 tattcataga tataaatgga ttaaaagata ttaatgataa ctttggacat gctagtggag
     7741 atgccatcat tatgtcagtc agtgtaatga tacaaaaata ctttcattct tattatgttt
     7801 accgtattgg aggagatgag tttgttgtat tatgtgaaga tatctcaaaa caattatttg
     7861 aagaattagt aagttggcta actgaagaac ttaaagataa catggaatgt agtgcagcag
     7921 taggaagtat ttggagtgaa gatgatattg atatacataa gttaattaaa gttgctgatg
     7981 agaggatgta ccaagataag aaacgatact ataataaaat cgaacttaga aataatgaaa
     8041 tgaaggtagt tagtgattta agcgattcaa caaataatat ttcacttaaa tgtagagatt
     8101 tagataatga agtgtatttt gatgatttaa tgataaaagg gtattctaag gcttatgaaa
     8161 aattagctta taatacatat gatataattt tatctatttg catggataca aatttaatta
     8221 ctccaattta tcaatcagag agcaatattt taaattttcc tataaaagag aatattgaga
     8281 aaattttctc atacattaaa aagcatttga ttcaccctga tgatataata ttatttgatg
     8341 ataacctcct tttaaaatct attgatagtc ctaataatag caaacaaggt gatttagaac
     8401 taaattttga gtatagacac ttaggaatag atgaaaagta ccattgggca ttactttata
     8461 ttctgaagat tagcagtaaa aataatttag gtgagaaaca aacacatatt ttagttgcta
     8521 ttaaaaatat agattatctt atgcaaatac ataaaaacca aaaggataca ttaacaggat
     8581 tatacaatta tgaaagtttt tgtgaacaaa caagcttaat gctgaaaaga tatcctggca
     8641 agcgctatgc aattataatg atggatatag ataagttcaa agtaattaat gatatttatg
     8701 gaattagagg aggagataaa gccttaatat atgttgcaga attgattcaa aaaaacattg
     8761 gttctaatgg tatatgttgt cgtatgtatg cagatgtatt ttgcattttt tatgagagtt
     8821 actctgatag agagattcat aaagtaattt ataatattaa aaataacttg aataggaaaa
     8881 aatttgaata tatgttagta ccatgttttg gggtttataa aattttggat aaccatatcc
     8941 caattacaag tgcatgtgaa atggcaggat atgctcacaa aaaagtaaag aaacaaagta
     9001 tcaatcatta tatattttat gatgatacta taagaaatga tgcacttgaa gaaaaacata
     9061 tggaaagaga aatggaatat gcacttgaaa atggacaatt tcatgtatat ttacaaccta
     9121 agtataatct taatacaaat aaaattgttg gggcagaggc attggttaga tgggttcatc
     9181 cagaaaaagg tataatacct ccaattaaat ttatttcact atttgaaaag aatgggttta
     9241 ttatcaaatt agacatgtat gtatgggaac aagtatgttc tttgattaga aaatggatag
     9301 atttaggtga agagccagtt cctatctctg taaatgtatc aagattacat ttgcataatc
     9361 cacattttaa agaaattgtt ttaagcttaa taaataaata tggtatacca aaatcttttt
     9421 tagaattaga gcttacagaa agtctattta ttaaaaatat aaaattattc actaaggtaa
     9481 tagctgacct tagaatggat ggtattgttt taaatatgga tgactttggt tcagcatatt
     9541 cttcattaaa tatgttaaaa aatattaata ttgatacatt aaagatagat tgtggtttct
     9601 ttaatggtga aggaatcaca gatagagaaa aaatagtttt aaagcacact atagccatgg
     9661 caaaagattt agatatggat gtagtggcag aaggtgtaga gacaaaagaa caagctgaat
     9721 ttttagtaag tttggattgt gtagtaattc aaggttatta ttattgtaag cctattccaa
     9781 gtgatgattt tgagaagtta ctttatcaaa aataagcgaa ttattccatt ctaaacgtag
     9841 gtaggatgga gtagttcaaa ataattaaat agaagcaaaa aatagtgagg atataatttt
     9901 taaaaagtat aatatttatg gattaggagc gaagtttatg atagtgggta ttataggtcc
     9961 ttcagactct gggtttaaga taaaggatga tttaaaacag atagacaatg atttaaagac
    10021 taaaatatat gtaagagaaa aagtggtaga tacaatagaa gttatttcta aatgtgaaga
    10081 tgaatgtgat gctattatat ttaccggatg tgcagtttat gaatttataa aatctaagta
    10141 tgagataagt aagccacatt catttgtacc gagaagtggg actagtataa tgaaggcatt
    10201 ttgggcaata aaaagtgcaa atataaaatt agataaattc agtatagatg tagttgatag
    10261 atatgttgtg gaagatgcgc ttaaagaatt tgatattaaa gcaactagtg tattttgtaa
    10321 tccattctct ttggaagtag gagagtctga acttgtagaa tggcatataa aattatttga
    10381 agaaaataag acagatataa tgttaactgg ttttggtgca gtatataatg aattaaaaga
    10441 aagaggctat cctgtatttc gtttacaagc tactatacaa ttaatcaaag aaagttatga
    10501 taaggtaaag agtgaatatg ctctaagcaa ggcaagattt tctcaaattg ctgtcgaaat
    10561 attgagtcta atagattata aagagaagat agataactat tattctgaca tgataaaaaa
    10621 atctgatatg gataagcttg tagtaaacta tgtaagaagt attcaaggtt ccttattttt
    10681 acttggaaga aatgattatg ttgtatttgt tcataaaggg gtagctgata atgagtataa
    10741 ttatgataag ctattcaagc taaagagaga gataaaagac atgggctttt ctcttagtat
    10801 aggaattggt actggagtaa ctgcgtatca ggctgaaaat aatgcacata aagcattaag
    10861 atactccata gattcaaaag aaattggtat atatttagta gatgaagatg aaaacataag
    10921 aggaccttta gcttcagaaa atgaattaaa ttattctttg atgctatctg atgaaaaggt
    10981 aatagaaata tctaaaaaga ctggaatgag ttgtgagtcg gttgcaaaaa ttattgctat
    11041 aagtgagaat agaaaaagta agatttatga ttcaaaagag ttagcagagt atctaaatgt
    11101 aagtgaaaga agtgcaagac gtatattaaa taaaatagta aatgcaggtt taggtcgaat
    11161 atgtgcaaaa gagactagta ttggtggagg aagaccaaaa aatattactg aaatactgtt
    11221 ttaagatact ttataaagct gtataaatca ttatatcata taaatttcta caacaaatta
    11281 ctatataata tattaaagct tattataaaa aatactataa caaattacta tataaaaaac
    11341 taaagcttat tattaaaaat gctagaataa attactatat aaaaaactaa aacctatgta
    11401 aagcaacttt ttaatatata acttgtaaag ttgaagatac ataggttttt atttttataa
    11461 tctaaagagt tatattatga tatttataat ttaatcatat gataaactat tttggcagta
    11521 tcatataatt catctatatt tacatattca ttaaccgtgt gcacatcata catacctaga
    11581 cttaatatag cacaattata tccaagatta gctaatatat ttgcgtcact tccaccacca
    11641 attaccattg gatttggaac aataccgact tgtcttattg cttcttcact taacttgaaa
    11701 acatgactat ctctactaag ttcaaaacta gggtaagaca tattatgttc aaaagtataa
    11761 gtagtattaa atttagatgc agcatcctta caacattttt ccatatgatt aagctcatac
    11821 tctaaagttt cagggatatg agacctaatt tcagcagtca atgtaaccct atcagttact
    11881 atgtttgtag ctccaccacc ttctatttta cctatatttg aggtagtcaa tgaatctatt
    11941 ctgcctatgt gcatattgct tattgcatga gaggctacta atatagcatt taaccctttt
    12001 tcaggttcta taccagcatg tgctttcttt ccatgaaaag agatttttat agattgttga
    12061 gcaggagctt tgtatgctat cgaaccaata gctccaccag aatctaaaat caccatatct
    12121 ttacaaggca agttttcagg attaaagttt ttgacaccat gcataccagc ttcctcacag
    12181 attgtaaaac aaagataaat atctctatgt ggtatttttt ctgtaataac atgttctaaa
    12241 gcttcaagta tagcagctat tccagcttta tcatctgcac caagtgtagt agttccatca
    12301 cttctaacta cattaccatc tagtactggc tttacaccta aacaaggttg aacttggtcc
    12361 atatgtgcac ataaacaaag tggtctactt ccttcttcac cctttatata ggcaatgaca
    12421 ttacctgtgt taccaccata tttttcacct gcatcatcta taatggcatc tatatttctt
    12481 tcttttaagt agtttaccaa ccacttagac atttctaatt cttgattaga tgggctatca
    12541 attctaacca tgtctataaa gttatttata agtctatttt tatccaacat aaattatttc
    12601 tccttttaga aagatttaaa taaattacat gctatatgaa ggaagtattc caggtcctag
    12661 tggaatatta aatatcataa acaatatcaa tagtatagtc cacactatta agaatgatat
    12721 agaatatggt atcatattag caattacagt acccatacca gcatctttat cgtattttct
    12781 tgtgaatgct agtataatag ggaagtacgg aaataatggg ctaagtggat ttgttataca
    12841 atcacctata cgataagcca tctgagtaag tgctggattg tatccaacca tcataaacat
    12901 tggtacaaat ataggtgcaa gaatagccca ctttgcagat gcactaccta taaataaatt
    12961 tataaagcat gataatataa tgaatcctat caataataaa ggtccattta ttcctgctga
    13021 ttttaaaaat tctccaccct ttacagctaa tatcagaccc aaattactat ttgtgaataa
    13081 ttgtaaaaat tgagaagcag caaatgctag tattatatat ccacccatat cactcattgc
    13141 actagccatt atagagacta catccttatc cttctttatt ttacctatag tttttccgta
    13201 tacaacacca ggtataaaaa atgctagtgc tattataggt accataccct tcataagagg
    13261 agcattgttt gatagtaaat ctcctgtttc cgggtcagct aagaatggtt ttttaccgat
    13321 tacactaaga gctactataa tagctacata tattaatata cttataccag cttttttaag
    13381 ccctttcttt tctacatcac taaggcttcc atcaacatct aattttgcat ttccttcata
    13441 tttaccaaat ctaggagcta caattttttc tgttacccat gttgccaaaa gtattaggac
    13501 aaaagttgat gctatcataa aaaataagtt cattgttgca ttggcttgaa atccagaatc
    13561 aatcatctga gctgctggaa ctgtaaaact tgcagcaagt atatcattga cgcttatcat
    13621 aatgtttgca gcaaatccta gacagactgc tgcatatcca gaaaataaac ctataagtgg
    13681 atgtctacca acaccaagat atacaagtgc agctaatggt ggaaatagta ttgtacctgc
    13741 atctgaagct atattggcaa gcatacctat agtcattaca acaagagtta ctcttgtttc
    13801 aggaatcttg cttactgatg ctttgattgt tgctgtcata agtcctgttt tatcacatac
    13861 accagcacct atcatagtta ctaaaactag acctaaagga gcaaaacctt ggaaattgct
    13921 aactatgttt cctaaaagaa tttgaagttg ttctacagtc aataaattta cagcagttat
    13981 agtttctcca gtaccaggat gaactactga tatccccata gaaccaacta cactagatag
    14041 tataagtaaa agtacacata accctaaaaa tatcgtaacc gggtctggta gtttattacc
    14101 aacagtttct attctgttta atgttctgtc aaaaaagttt ttcttcttaa ctacgttatc
    14161 attcataata tcctccctaa tatttaaaaa tataattaaa aaataagtat tgtggttaag
    14221 ctttcatgta aattctgctt gttgatttaa gacgtgtcca caatatgtcc taaaaaaagt
    14281 ataaaataaa ctttttccta tgtcaatgca gttttctgaa ttttctggtt gagtagtgtt
    14341 agttaaatat actttaagta gatttatgat gaattgtatt taatatatct ttgcagtata
    14401 taatctttaa aattaatttt aaaacaaaat tttataaaaa gctagtggaa gattaagtta
    14461 ttgattttat tgagttttat taaagataat attttttatt ttaattccta tattagggta
    14521 tttattttgt ttaaaagtta aaaggacaaa tagtaggcag atataacaat aaatgataat
    14581 aaggatatct ttctcaaaat agatatagac ttttttataa ctaaaactat aaaaatatat
    14641 ttgatttcat acaaaaaaga gtgtctaata aactaaaaaa ttcatcttta gacactcaat
    14701 tttgtattat aaaaatagac ttataaagtt ttatcgtgtt tttctaggaa tgttagatat
    14761 tcttctttag tcataactgg agtaaaggca tccaatatat ctttagcatt cttagcattg
    14821 tcaaaaagaa ggtctacaac tgtaagtgcc attgctttag caggaactat gtaagctaaa
    14881 tcttcatcaa ctatttcaaa atcccttgta tgaagagctc cttttacacc accaatcata
    14941 gggtgaagag taggcattat atgagacaca tctccaaaat caaaagaacc tgtaaaatct
    15001 ccaccatcaa taacttttcc ctttagacct aaatcttcaa gattgttttt aaataaatta
    15061 tcaaggcttt tgtatcttaa tattggaaga tatccaggaa tttcagttat cttaacatca
    15121 gctccaactg ccatcccacc agccatcaaa gccttattta ttctttcatt agcatcaatc
    15181 ataccattta tagttctagc tctaacatat gattccatat gaacgtcagc aggaacaaca
    15241 tttactatat ctccaccttt tgtaatgata gggtggaatc taactctttc agactcttta
    15301 aaagtttctc taagtgcatt tacattattt atagcaagca ttgctgcatt taaagcattt
    15361 attccatcat gtggagcaga accagcatga gattctttac ctataaattt aacttttttt
    15421 ccaacaaatc cattactttc aggaccaact agagccttat cttctcctaa atcagaagaa
    15481 tgaaacatca tagatatgtc tatatcatca aaataacctc tttttattaa ctcttgtttt
    15541 ccaccaaaat aagttatttt tccttccttt tttaattgtt ctctgtattc caactcaata
    15601 aactcttcag ctggagtagc cataaaagat actttaccat ctaaatgttc taaaacacca
    15661 cttttcacta aaccaacagc ggcacctaac attccagcta tttgaatatt atgtccacaa
    15721 gtatgagaag cacctatttc attagcatct ttatgttctt tacatgatat accatccaat
    15781 tctccaagta tagatatatg aggaccttcc ttgctagaat ttgcatctgc tttacatcct
    15841 gtaactgcta tgtttttttc tacagataag tttaattcct gctcaaaaaa gtttgaaaca
    15901 gcttccgtag ttttaaattc tttatatcca tattctggat tattgtatat tccttgacct
    15961 atttttaata ttttttctct attttcatct atagttttta ttactagaga ttttaattgt
    16021 tctttattca taataaattc ctcctatatt ttaccttgaa tacttaaaac tatatgagct
    16081 atactcgcac atgcaaagaa tatagttgta aatacaataa gtgagattat aactattttc
    16141 catgacattt cttttaaagc ttctattttg tttccaacag aaattcctgc aaaagctagt
    16201 agtggagtag ttatagccat aaagttaata ttattagtgt tagttaagaa tacttcagat
    16261 gttggactat atggcatact aagtaaaagg cctattatag ttgcaaatcc aaaagctgga
    16321 aagattgatt taggaaataa atcttttata atcatagccg ccatagcagc taatattagc
    16381 atcaccatac caggaagagc tgtaactata gatgtaccaa cgctaattct ttgtccaata
    16441 agaattataa ccccaataac agacataacc aataattgat atgaactttt cttcaaattt
    16501 aaattagcaa acatttgtta gcactctcct tttgtagatt taatttcttt agtattttta
    16561 tttctcgaaa atttaggttc taaaatctta tacataaaat tagtaaatgg taaagataca
    16621 aaaactaaga atataattcc tgttatgcta gacatcatat tgcttgttgc agcataggca
    16681 agtattgtat ctgccatttc aggtggtacc atggtagaca atgaaccagc agctgccgtc
    16741 atcatacttc cactaccaac accacaggcc attcccaaag catatgggtg aagaccactg
    16801 tagatagata ttgaacccag gacactaaag tatatagtac caagaactgt tcccattaaa
    16861 tatgtaccaa gtacaccact accttctgga gagtttatcc catatttttc tgatataact
    16921 cctaaagaag gttcacggct tatacttgca gtagctccaa cagcttctct tttcattcca
    16981 aacattaatg caagaggtaa tgcaacgatt ggtgcaagta tatgaccaaa ttcttgggca
    17041 atgaatgctg gtccggcttg aattatcttg tcaatgtttg gtccaactgt agtaccatac
    17101 tttataccaa gcattactag tgcaattcca acaacttcac cagcaatatt tatttctttt
    17161 tctccaatta cttttttaag tgctttaatt tttttaccta ataaatctgg tgttattata
    17221 agtccaatta taactgcata aagcataggg aacagtacta tagaagcttt tccaatagct
    17281 atagttcttt gccctataaa ttcacatatt actactaaga ttaagcaagt taaaaatact
    17341 tgaataaaat gttttttatt aatttccatg atttcctccc caaaagaata aatattgtac
    17401 ttatttgaaa tgataaaata cgttttctta agtataatac gttttcctaa atgaaataaa
    17461 aaaactattt caaataagtt tcagaaatgt caataacatg tccttaatta aggataaaac
    17521 aaaaatttaa cattgtcaac gaaaaatatc taaaatatta catattttga atatattgta
    17581 atatggcttt attatgaaaa agttgtttaa atattgtatg agtaataatt aaaaagcttc
    17641 aaaattagtg atataaaatt taaaaataat aacatagaat tgagaaatag taaattgaaa
    17701 ttttaaaata aatgagtgag agtggtttag aaagcattga aatgaatagt ataataaaaa
    17761 ataaatacta tggaaagact ttgaattaat tgtaagtatt aagtatagat actaattttt
    17821 gatatagaaa tttaataaag taaatgataa taagcaagtt aagtaaaatg atgacaaatg
    17881 atattataat aagggaattg acatagcaat aagtaaatta agtaagatag taataagtaa
    17941 attaagataa taatatccaa agtgagaaag ataatagata gtaaataaaa tcatataaga
    18001 ataatctaga taagattcta agattttatt tgcttaaaaa ttggtataat taatttagat
    18061 taaataagtt taataatttt agtaggtggt aatgaatgaa aataaataga ttaacagaaa
    18121 taatagtaat tctattaaat aaaaaactag taacagctaa agagttggca gatagatttg
    18181 aagtttctac aagaaccatc tatagagata tagaaactct gtctatgtca ggagtaccag
    18241 tatatatgat gaaaggcaaa ggaggaggta tatctttaat agaagaatat tctatagata
    18301 aggctatatt gtcaaaaaaa gataaagaga gtttaatagt ggcgctgaaa acattacagg
    18361 cgactaagta tccagaaata aattcagtag taaataaaat tggttcaata tttggagagc
    18421 agaatttttc aaactggatt gaaatagact ttactgaatg gggaagcaat tttaatgaag
    18481 atgataaatt tacaaaaata aaagaagcta ttttaaggcg taatattata aattttaatt
    18541 atgtaaatag tttgtcaagc cagacaaaca gaactgtcga accacttaag cttatgtata
    18601 agagtaagac ttggtacttg tatggatttt gtaagctaaa agatgatttt aggatattta
    18661 gaattagtag aataaggaat ttatctataa aggaagaagt gttttcaaga aaggtaatag
    18721 aagaagtatg cttgaatgac tctaaagtaa ttaaggaaaa tactataacg ttgaaattga
    18781 ggtttaaaga aaagatgctt tttagagtat ttgatgattt taataaagat ttaataacta
    18841 aaaatgaaga taatacatat gatgtaatta cggaatttcc tattggtgaa tggatttatg
    18901 gatatatttt atcctttggc gacaacgttg aagtacttga acctaaagat gttagggata
    18961 atgtaataac tagactaaag gagttgtcta aaatttattc cttataatat aatgctagat
    19021 taaaaagttt attaggtata ttataatatc tcactgagtt taattatatt aaaaatatga
    19081 cataaaggtg tcgtatatta taaattataa ttgaattagt tcaaagtaaa atatattaat
    19141 aaaaacaaag tgaggtataa agtatgaatt atgaaatagt agagataagt gagaaaatag
    19201 ttataggaat atcaaaggaa actacaaata aagatggaca agcagtaaat gatatagggg
    19261 aattgtggaa aaaatttatg ggcaaaggta tatataatgc tataaagggt aagaagagtg
    19321 acaagacaat aggtctatat acagattacc agggtgattt tacaagtcca tataactttg
    19381 ttgcatgttg tgaagtaaat agtaattctg ataaagataa gaatttagaa gaattaaata
    19441 ctaataatac tgttggagaa attattatta gtaaagttat accagcaggt aagtatgcaa
    19501 agtttgttat agcaggaggt caaaaagaag taggagattt ttggttggag ttttggcaaa
    19561 tggattttga caggacttat gtaagtgatt ttgaagagta tcaaagtaat acttttgata
    19621 caaataaaca tgaaattcat atttacatag gaataaagta gatttgttac tattatattg
    19681 aatgaaggaa gtatcgagta tagtagattt attatagaaa gtttaattat taaaaaagca
    19741 gtaaatcttt tacagattta ctgctttttt aataattaac agtatcatta tcactttgaa
    19801 tatttagaca tattcttcat ttaatattgt ttgttgtaaa atgatacttg taaatataat
    19861 gacttgtcat ttaatttgaa atatgaaaaa aagcattata agtatgtgat aacacttttg
    19921 gatttatgag tatgcaattt gaaaaatata aaaaataatg tataaagcta aatataattt
    19981 aatcgataat acatatatag aaatataatt aagtaaataa acaactatct attatatatt
    20041 aagaaagtca aatcttatat aaatctacag aaatgatttt aatgaatctg aaaattattt
    20101 ttatacaaat ataaataagg tataaagtta tgttttatta taatttgatt acttttgatg
    20161 ttttatacaa ttatattttt aataactcgt caaaacacca aacatatatt acaatagata
    20221 tataagtttt tttgttgaaa aattatgtta agagaattaa gcataatatg acataagtaa
    20281 ttacttttat ttatcaatga aatgtattta ttttgtagcg aaaaatagta agatagtata
    20341 gataataact gaaaataaaa gcgtttattg ttgtacatat ggaggagtta gttttatggg
    20401 aaataaaatt ttgatagtag atgatgctat ttttatgaga atgataatta aagaggctct
    20461 gggtaaaagt ggctataata atttagtaga agcaaatgat ggagaagaag cgtgtaatat
    20521 atttaaatat gaaaagcccg atttgatgtt gcttgacatt acaatgccaa aaaaagatgg
    20581 tttagaggtg ctaagagaaa taaaaaagtt ggattcaagt gctaaggttg taatgtgctc
    20641 tgcaatagga caagagaata tgattgtaga tgccataaaa cttggagcat tagattttat
    20701 agtgaaaccc ttcaaaacag aaacattagt taaagttgta aataatgctt taaaataaaa
    20761 gtgtaaatat atactgatac aataaaaatc aaataaattt aataatagag ggaggggttt
    20821 atattataaa ttatttagaa cttgatgatg ctcatataga tgctcttaga gaaattggta
    20881 atataggctc tgggaatgca attacttcac ttgcatccat gataaatagt aatgtagaag
    20941 tattaatacc tatggtcaag atacttgaat ataacgaagc gacaaatttg cttggtggtc
    21001 cagaaaacaa agtagtatgt attttactcg atatgaaagg cgatattaat ggaatgttta
    21061 tgtttttgtt ggatgaatca ataactcaac ttatgctaag ttctcttttt aataaagaag
    21121 aagttccttt agatgatatt gaggaaattg aaatttcagc aattaaagaa ataggaaata
    21181 ttatggcaag ttcatatgta aatgctattg catctatgtt aaacatgact ataagtgttt
    21241 caattccaga tatatgtatt gatatggttg gagctgttct aaatgtgcct atgataagat
    21301 tttcagatgt tggtgacaaa gttttatttg tagaaaataa gtttaaaatg agtgataatt
    21361 actttactag tcacattttg atgattccag aaatgagctc acttagagaa atactagtga
    21421 agttgggatt agagatatga atagggagat agtagtgaac attgcagata tgaagatagc
    21481 ctatagacca aatgttttag ttacttatgc acttggttct tgtgtaggag tatgtttgat
    21541 agataaagta gctggaattg gtggaatgtt gcatgttatg ttgccatata gtaaagatgc
    21601 tatcaacatt gaaaataagt ataaatttgc tgacacagga ataaaagagc tcattaaatc
    21661 tatggagaat ataggtgcaa atagaatata tataaaagca aaaattgctg gaggggctca
    21721 gatgttttta ggaaatagca attcagcaat tgcaagtata ggacacagga atgttgaggc
    21781 agtaaaaaaa actcttttag gattaaatat tccaatatta gctgaggaca caggtatgaa
    21841 ttatggaaga acaatacgtt tttatactga aacaggtgaa ttaattgtag attcaataaa
    21901 taaggggaaa aagaaactat aatatagatt ttatagaaag tatattttag tagggaggta
    21961 ttaaatgaga tttccagatg acccatattt agattatgga ttaattgatg aaggaattaa
    22021 gtatttaaag tttaaagtag atgaacaaga ttttggggtt gaacttgaaa aagtaataga
    22081 aattactgga aatcaagaaa ttatatttat tccagaacta cctacatatt ctaaaggtat
    22141 aataaatcta agggggaaaa ttattccaat aatagatatg cgtttgagac ttaaaaaaga
    22201 tgaattaatg attacaaatt gtatgtatat agtagtaact gaaataaaag acttagtagt
    22261 tggatttgtg gttgatagag ttgatgatat aataagccta gaaaaaagtg aaatatctcc
    22321 tccaccaaga gttactatag aatattcaga ttattttgta agcggtattg gagaattaga
    22381 tggtaaagta attactttac tagatttaga gaaactttta acagataaag atgaaatttt
    22441 aatagatgaa ttaatcaatg ggtttgattg cactatcatg taatataggg atgtagttgg
    22501 aatgttatta taaaaagatt gttatgagaa catttaagaa tatagtggta taattattaa
    22561 gtataaaaat tttaaataat taaaatttaa atatattatg tttaagagag gtttgtatat
    22621 gaaaaataag caacagggaa caattttagt cgttgatgat agtgttctga tgtgtgactt
    22681 aatagataaa gcacttacaa aagataattt taaggttaaa aaagcattta atgcaataga
    22741 ggcaaaaaag tttattgaag aatttatacc agatattatt ttgcttgata taattttacc
    22801 agataactct ggttttgatt tttgtaagga agtaaaatct aatgctcgca cagcagatat
    22861 acctataatt tttataactt caaaaaatgc agatacagac atagtaaaag gatttggtgt
    22921 aggagcaatt gattatatgg tgaaaccttt tagtatgaca gaattaaaag ctcgtgttat
    22981 agctcatctt gaagtaaaga aatctcaaga taaattaaaa aagataaata atgaactgga
    23041 atcttctctt gaaaaattga atagactcgt tgtaagagat tatcttacag gtctttacaa
    23101 taggcgacat ataattaatc aacttatgga gcaaagaaga attaataaat atagtgaaac
    23161 atcaatatcc cttatccttg gtgatattga tgactttaag gttatcaatg atacttatgg
    23221 acatgaagcg ggggacttcg tgttaacaat aatatcaagt ataataaaaa agaattgtag
    23281 acaaattgac actgtatcaa gatggggtgg agaagagttt ttgataatgc tcacaaatac
    23341 tccaatagaa gacgctaaga tactttcaga aagaatccgt aaagaaataa aggagtttga
    23401 tttcaactat aaagaaaata aaataaagtg cactattaca ttaggagtag tagaagtaaa
    23461 ctcaaatata tcattagaag aaaatatatc cttagcagat aaagtaatgt atgagggtaa
    23521 aaacttagga aaagattgta gcgtagtgag tacaatgaaa ggattaaaaa agatataaac
    23581 ttactttgta aaggagtaca gatatgaaaa gagaagtaaa gctttctaaa ttagcaattg
    23641 tattgctggt tattatagta ttggctgttc tagcagggat tttttcttct atttcatctt
    23701 ataaagcatt tgaaaaagta gaaaaaagta ttaatgatat gaataatttg agaactcaaa
    23761 gtcagtatat aaaagataca actagagaga taagtgatgt attaagaaga tatgtattta
    23821 caggagaaaa aaagaataaa gatatttatg aaaaagaatt gtacggaaaa acaagagaat
    23881 cagctttgaa caaattagag aaaattggtc ttacaaagag cgagtcaaat aaaatcttag
    23941 aagcaaaaga aatatcagat tctttggtac caacagaaca aagtgcaata aaggcagtcg
    24001 aaagtaatga taagagtaaa gctcaaaata taatttttag tagtgattat ataaactccc
    24061 aagagaaaat ggattcttta ataaatgaat ttcaagagga tatagttgcc cgttcaaatg
    24121 ccaaaattgt agaagtaaaa aaagaattac aaagtctaat attaagaaca atatttatac
    24181 tttctctctt aataatactg acaataatag aatatacagt aataaaatta aaaatagtta
    24241 ttcctattaa taaagtagaa aaacactttt caagaattgc atctggggat ttaagaacaa
    24301 gtatagacgt tgaagaaagt aaatctgaaa tagggctgtt agtgagttct gtcaaaaaga
    24361 tgcaaagtat gcttttaaat tatatagacc ttatagacag tacattaact agtattgcaa
    24421 aaggagattt aagagtagaa atagaccaag aatttatagg tgattttaaa tcaataaaaa
    24481 tatctttaga aagtataata aactctttta atggtgattt ttctgaaata aatttatcag
    24541 cagagcaagt tgcaagtgca tcagaacagg ttgcagcagg tgcacaagct ctttcacagg
    24601 gtgctactga acaagctagt tcagtagaag aattggcttc cacaattaat gaaatatcag
    24661 atggaattaa gaagtgtgca gatagtgcta gtcttgcatg tgaggtatct gaccaatctg
    24721 catcacaagt tgattttgga agtagatgta tggatgatat gatgacatca atgggtgaaa
    24781 taagtgaaaa atcgagtgaa atatcaaaaa taattaaaac aatagatgag atagcatttc
    24841 aaactaatat tcttgcatta aatgcagctg ttgaagcagc aagagcaggt caatatggta
    24901 ggggatttgc agttgtagca gatgaagtta ggtctttggc tggaaaaagt gcaaaagcag
    24961 ctcaaaatac agctattctc atagaagaag ctattaaaac agtagaaaat ggtgtcaaaa
    25021 ttgctaaaga gactgcaaat gcattggagt tggtagtaaa tggtgtagaa aaaactactg
    25081 attatattaa tggtatttct tcagctgtac atagtcaaga agtagcaatt acacagattt
    25141 tatctggaat agagcaaatt tccatagtag tccaaatgaa ctcagcaaca gcagaggaaa
    25201 gtgcagcagc ctcagaagag atgtctgctc aagcgcaaat tttaaaagaa ttagtagaaa
    25261 aatttcatct aaaggaaagg gaagtaagag aatcatcatg ggtttaaatt atgaggtgca
    25321 tatataacga aataagagag ttattgtata tttaaattat gaggtacata tatagtgaaa
    25381 taatagaatc atcaaggatt taaattatga gatgcatata taatagaaaa ttatttgtag
    25441 gtcgtaggtg ataaatgtga attatattga accgagtatg agacaaatgt tagatatgtt
    25501 tatatttgag acaagtctat tactcgaaca attagatgaa attatgattc aaatagaaaa
    25561 agagaaagta tttactcata acaatattaa tgaaactttt agaataatgc atacgataaa
    25621 aggttcagca tctatgatgg ggttagacaa tatatctgct ttagcacata gtgtagaaga
    25681 cttgttttat gctatacgtg aaggtaaaat agaagtttta gacaatgacc tattatttga
    25741 tttagttttt caatctttag attttataag aagagatata gatgagttac aatcaagagg
    25801 ttgtatatct tctgattact ctaattttat cgaaggaatt gaagattatg tgagtacttt
    25861 ggaattatca agttctatga aatcaaagga taatgcaagt ttagaaagta cagatgaaat
    25921 agaagtaata gacttaaatt ttgcatacag agaaggttct gatgtagtaa aaataatctt
    25981 tgaacaaggt tgcatgatgg aaaatgtgag agctttcatg cttgtatttg aacttagaga
    26041 ttactgtgaa tttctagatt tttatccaaa agatgtagaa acaaatgcag agtctacaga
    26101 atatattaaa aattatgggt tttatataca atttgttgct aaagaggata attcaatagt
    26161 ttataaaatc atagaagaga atctctatgt aaaagaatat ataaatatta gtaaaaaaga
    26221 aatagaaatg aaaagtacat caaacaataa tgaagtagaa aacaaattaa aagatagtaa
    26281 caaagtaagt aaaagttata aaaataacta tacagaagat gagttacaag aagatgtttt
    26341 aaatgaaaaa actgatatag aaaaagatgg tctaagtgga gaaaatgaac atttaccaat
    26401 aagtaagcag agtattatga gtgtaaattt agaaaaattg gacttacttc aaaatatagt
    26461 tggagaaatt gtaattatgg aatcaatggt tgtaaatagt gtaaattcta caggtgttga
    26521 tatagataat ttcaataagt caacaagaca attacacaag cttacagatg aattgcaaga
    26581 tatagtaatg tctatgcgta tgattcaaat tgctggtgta tttcaaaaaa tgcatagaat
    26641 tgtacgagac atgaataaaa aattagataa aaatgtagag ttaataacta taggagcaga
    26701 gacagaggta gataagagtg taattgataa tttaacagaa ccttttatgc accttataag
    26761 aaatgctatg gaccatgcga tagagcctat cgaagaacgt atagctaaag gcaaaaaaga
    26821 agtgggaaag atagttttat ctgctgaaaa tttaggtgga gaagctgtga taagtatttc
    26881 agatgatggt agaggcatta tgaaagacaa gattttagca aaagctaagg aacaaggtct
    26941 ccttaaacat caaaccaatg agtatactga tgaggaggtc aattctttga ttatgactcc
    27001 aggtttttct acaaaagaat ctataactga attttctggt agaggagtgg gtatggacat
    27061 tgttcgtaaa agtattgaaa aggttggagg ttcaattgaa gtaaaaagta aaccagatga
    27121 agggacaact tttactataa aaataccact aactctttct atagtagagg gtatgaagtt
    27181 taaagttgga gattcactat ttactttacc aatatcatct ataaggagaa catttaaact
    27241 tacagactta aatcaaataa ttacagaaga aaataaaaca gagttaatta caatatatgg
    27301 agcatgtatt cctattataa gattacataa aatatataat attcaaacaa aagtaaccaa
    27361 tctgatgcat ggtaatatga taattattga aaatgagaat aaagcagtat gcatttttgt
    27421 tgatgaaata ataggtaatc aacaagttgt tgtgaaacca tttccagttt actttaaccg
    27481 attcaatata aaaaataagg gtttatcagg ttgcacaata cttggggatg gaagtataag
    27541 cttaattata gatgcagata gcattataga aatatattag gggatttaaa gtatggctaa
    27601 agatgaaatt attaaacaag tattaacatt ttatgtcaat gatgttattt atggaataga
    27661 attagaaaat gtgattgaga ccataagatt tcaatcaata acgtatgtac catgtcttcc
    27721 aacatatata agtggagtta ttaatttgcg aggaagaata ataccagtta ttaatatgca
    27781 tataaagtat aatcttcctg aagctgatta taatgagaga acttgtataa taattacaaa
    27841 ggttgatgag tatcaagttg gaattattgt agatagagtt attgatgtta ttcctataga
    27901 aaatctaaat ttgctagcta caactaattc taacaatatt aatattaata aagatataaa
    27961 agaaattgcg aaagttgaag ataattctat attaatttta gatattcgaa agtttttgat
    28021 taatagcata tagttagttt tgataaatta gcaggaggaa gtttttgcat gataaaactc
    28081 acagatgaag aatttatagt tcttgtaaac catgtaaaaa aagaatatgg tattgattta
    28141 agcaaaaaaa gagcgttaat tgaaggtcgc ttatacaata caatgataga gagaaaactt
    28201 agttctttta gtcaatatat gaatctatta tttaaagata agacaggaaa tgaagctata
    28261 aacctaataa atagattgag caccaatcat acatttttta tgagagaacc tcaacacttt
    28321 gagtttatac agaatagtat atttcctttc tgggaagaaa ataataaaat aagaaacata
    28381 aatatatgga gtgcaggctg ttccagtgga gaagaagcgt attcaatagc tatgactttg
    28441 gatgattatt ttggatataa taaagagttg tggaacatta aaatcatagc cacagatatt
    28501 tcaactaact cacttgaaaa agctaaaaga ggtatataca tagaatcaaa tgttaacaat
    28561 gttcctgagt tatggaagaa gaagtatttt atagacaata aggatggtac atttaaaatt
    28621 tgtgataaga ttagaaaaag tgtaattttc aagccattta atttaatgaa tgatttttca
    28681 tatcaacatt ttgatttgat tttttgtaga aatgttatga tttattttga tttaaaaaca
    28741 agagaagaac ttataaataa attttataat gctaccaaaa aagatagttt tttgtttgta
    28801 ggacatgcag aggtaattaa cagaagtaat actaaatata aatatgtaaa acctgctata
    28861 tataaaagat aaaatgctag ttttgatata tgattttgtg gaggcggtaa tgtgggacgt
    28921 tttaataata tggttattgc tataggcgca tctgtaggtg gtacagaagc aatacttgaa
    28981 attataaaag acttaccaaa atcaacacca gggattgttg tagttcaaca tatgccagct
    29041 atatttactt atatgtatgc acaacggctt gacaagcagt gtattatgaa tgtaagagaa
    29101 gctaagaata atgatagagt cgaacaagga aatgtattaa ttgcacctgg gggttgccag
    29161 atgaaattgt gtgcggataa acaaggatat tatgtaactt gtgaaaaagg agaaagaata
    29221 agtggtcact gtccatcagt agatgtattg tttgattcag tagcagaagt agcaggtaaa
    29281 aattcgatag gaataatact tacaggtatg ggttatgatg gagcaaatgg acttttaaaa
    29341 atgaaagaaa atggttcatt tacaattgga caggatgaaa attcttgtat agtatatgga
    29401 atgccaatgg tagcttttga taggggttct gttatgttac aattacctct tggtgaaatt
    29461 tcaaactgtt tgataagaca tattaatatt ttaaaacaaa aagattaatt ttttataaat
    29521 aaaaattaat cttttattaa attatatgga taaaaaaccc tagaaactcc aaatataata
    29581 agagagattt atatttagga ggaaaatgat aatatgaaat tttttaaagt ggatacaaaa
    29641 gctaaagttt taagatatgt atttgtttta accttgacta taatggctgt tttggcaatt
    29701 aaacatgact ataaatataa taatttgaaa aatgcttcaa ttaatttaaa taataaggaa
    29761 gcagtgagcg ttatgagtaa tcagtcatta ccaaagggta atacaaatag taaacaaact
    29821 ttatcagtaa gtacaatagg aagtaaaaca atagtgaaaa atgatgagta tttagaaagt
    29881 actataaaca tgcctgtaat aacaaattca aataagattg ttgaaaggtc tacaaatgat
    29941 aagataaaaa atgacatatt tgaattttat aataaatcat ataaagaagc aaagcagtat
    30001 cttaaggata atcctgatga gaaaaataag tttgttgcaa atgtagattt tgaacttaaa
    30061 aagaatactg attctgctct tagtataaaa gtgagatatt atacttatag tggtggagca
    30121 catggatttt atcaagatac agcatataat gtggatatga gaactggaaa atttttagaa
    30181 ttaatggact tatttaagga taatactaaa tataaagaag ttatagatga agaaattaaa
    30241 agacaaatag ctgaacttga aaaaaaggat gaagaaaata tcggaatata taattttaaa
    30301 ggtataaaag agaatcaaaa cttttatttg caagatgata atctagttat atattttgat
    30361 ttatatgata taactccata tgcagcaggg attccagaat tttcaataaa taaaaaactg
    30421 ataagctcta tgctaaaacc agaatatgta gatttatttg atttgaaata gaagattaaa
    30481 ttaaatgtta tgctataata tttaacatag agacttatta aaattggggg gtataaaaat
    30541 gctactttta aaaaaagacg acataaaaaa agtatttaca atgagagatg caattgaagc
    30601 tgataaggaa gcttttagaa tttattgtga aggaaagagt gtaaatccct tgagaaccaa
    30661 tataccagtt ccaagtcaag atgctagtat gttattcatg ccagggtatg tagaagaatt
    30721 agggtgtggg ggaataaaaa ttgtatctgt atttcctaaa aatgctcaaa aaggcaagcc
    30781 agtaattcca gcaacagtac tactattaga tggtgaaact ggagaagtaa gtgcagtatt
    30841 ggatggtact tatgtaactc aaattcgtac cggagcagca tctggagcag ccatagatgt
    30901 tttggctaaa aaagattcta agattggagc tttgataggt gttggaggac aaggtgagcc
    30961 tcaacttgaa gctatgatag aagctagaaa tttggatgag gtaagagttt ttagtagaaa
    31021 caaggaaaat agagaaaaat ttgtagaaga aatgaattta aaattaagta aatataatac
    31081 taaaatagtt gcagttgaat catctgatga agccatagat aatgcagatg taatagtttt
    31141 agctactcca tcaaaacaac cagtatttaa tggaaactta gtaaaaaaag gagctttaat
    31201 aagtgcagta ggctcatata tgccagacat gcaggagttg tgtccaattt gtttaactag
    31261 agcatctaag atattttttg aatcaacaga tgcagtttta tctgagtcag gagatatatt
    31321 gattccatta aaagaaggga agataagtaa agatgatttt agtggtgatt taggaaatgt
    31381 tataaatgga actattcctg gcagagaaga tgatgatgag ataattgtgt ttaaaactgt
    31441 cggaataggt gtacaagacg tcgtaacagc aaaaagaata tacgacaaag caaaagaaaa
    31501 cggtatcgga atggactgga aatggtaaaa tattcaaaat aattctatag ttagataaat
    31561 ttatgttata atgttgataa ataaattttt tattatttta attgagggag gatatttgta
    31621 tgaaaatggt aaaaaagtta atattatctt ctattatggc tctcgtttta gttctaacta
    31681 tagggtgttc taatgcagaa gaaaaagtaa agtcaaattt tgaagctaat ttagctgcat
    31741 tagaaatgta taagtatgat gaaaaaggaa ttgctgatgc attaacagaa gctgaatata
    31801 aacagattca aacttataac agcaaggata aggtttcaat gattgctcag ttgacacctc
    31861 tttatggtaa agaaaatgct gaaaagatat acgaaacaat tgttaagtta attcaaggtg
    31921 ttgaaaagac tgtaacagtg gaatcttcta gtaaggataa gataactttg aaagttgtaa
    31981 gcaaagcttt agatactagt aaagtaatga gtgaagcgaa caatttagta aaatcttatg
    32041 taactgatga aaagaattac gataaaaaag aaaaagaagt tcaagatgat atggcaaaaa
    32101 tgattataga cctttacaat aagaagccga ctaaggaagt tacaactatg attacatata
    32161 ctaaaaaaga tggtaaatgg caaccaccta gtaatataca aaagttatgt gaaggaatat
    32221 atacaattta atttatttta aagacacaaa actatctttt aagcatataa taaaatgttt
    32281 ggaagatagt ttattttatt ctcaaagtat aaattatatt ttataagtga tttattttat
    32341 aaataat
//
LOCUS       DAMJVM010000039        31053 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.040, whole genome shotgun sequence.
ACCESSION   DAMJVM010000039 DAMJVM010000000
VERSION     DAMJVM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31053
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.040"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(89..2059)
                     /gene="ftsH"
                     /locus_tag="PC523_001889"
     CDS             complement(89..2059)
                     /gene="ftsH"
                     /locus_tag="PC523_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDF2475414.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(2093..3481)
                     /gene="tilS"
                     /locus_tag="PC523_001890"
     CDS             complement(2093..3481)
                     /gene="tilS"
                     /locus_tag="PC523_001890"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDF2475415.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERSEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVSEE
                     IEFYYNIPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(3664..4092)
                     /locus_tag="PC523_001891"
     CDS             complement(3664..4092)
                     /locus_tag="PC523_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDF2475416.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVV
                     YKEKEENIEITNTIKILKNEVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RKLDINIQEGEHIKLKIDYNINVQDLFLGRNKIDIYIDTK"
     gene            complement(4109..4915)
                     /locus_tag="PC523_001892"
     CDS             complement(4109..4915)
                     /locus_tag="PC523_001892"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDF2475417.1"
                     /translation="MSNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKV
                     LEVKNIINNTEIHGTYQYYVSDIPEKFTDIAREFLKKKIDRIQKVEIQKY"
     gene            complement(5117..5602)
                     /locus_tag="PC523_001893"
     CDS             complement(5117..5602)
                     /locus_tag="PC523_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HDF2475418.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTAKIEKINKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(5653..6312)
                     /gene="spoIIR"
                     /locus_tag="PC523_001894"
     CDS             complement(5653..6312)
                     /gene="spoIIR"
                     /locus_tag="PC523_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HDF2475419.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILENGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVIEVVKDLEKK
                     F"
     gene            complement(6458..7174)
                     /locus_tag="PC523_001895"
     CDS             complement(6458..7174)
                     /locus_tag="PC523_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2475420.1"
                     /translation="MLLIAVKKGLKFVENLTKLNLDNYKPLRDVVFENLREAILEGKL
                     KPGQRLMEVQLAEQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLE
                     VRASLEGLAAYLAAERISEEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKA
                     TNNKKLIQLINSLWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYAT
                     EHIEKAEQFMIDKAMISKDL"
     gene            complement(7122..8012)
                     /locus_tag="PC523_001896"
     CDS             complement(7122..8012)
                     /locus_tag="PC523_001896"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDF2475421.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELEEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNQLWKLRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTSIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTISKHKEISDIKQIMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(8130..9680)
                     /locus_tag="PC523_001897"
     CDS             complement(8130..9680)
                     /locus_tag="PC523_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HDF2475422.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVVQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(9773..10039)
                     /locus_tag="PC523_001898"
     CDS             complement(9773..10039)
                     /locus_tag="PC523_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDF2475423.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(10187..11047)
                     /gene="yabG"
                     /locus_tag="PC523_001899"
     CDS             complement(10187..11047)
                     /gene="yabG"
                     /locus_tag="PC523_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HDF2475424.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(11131..12069)
                     /locus_tag="PC523_001900"
     CDS             complement(11131..12069)
                     /locus_tag="PC523_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HDF2475425.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMATKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLNKFYDLGVRMMTISWNHVNELSFPHNKLEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDEIEKIYYKNALRVIKEVL"
     gene            complement(12179..12340)
                     /locus_tag="PC523_001901"
     CDS             complement(12179..12340)
                     /locus_tag="PC523_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475426.1"
                     /translation="MFILRFADDDDNLSVKDFKSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(12350..12847)
                     /locus_tag="PC523_001902"
     CDS             complement(12350..12847)
                     /locus_tag="PC523_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDF2475427.1"
                     /translation="MSLYTEWRNLSDNHENQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEDVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(12962..13672)
                     /locus_tag="PC523_001903"
     CDS             complement(12962..13672)
                     /locus_tag="PC523_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HDF2475428.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNNVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWIKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYNVDINNIKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFGLDVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     DQEYAIKVLKELFNYDII"
     gene            complement(13845..14375)
                     /locus_tag="PC523_001904"
     CDS             complement(13845..14375)
                     /locus_tag="PC523_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475429.1"
                     /translation="MDIRALPTKITKEEAIDIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIVTLKIHKTKSIFKSRRYSNGVNELKILVNGSTGSGSIVQDFPTYEIIKNVDCN
                     IVQYSDKDERRVKAKANKIAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(14392..14622)
                     /locus_tag="PC523_001905"
     CDS             complement(14392..14622)
                     /locus_tag="PC523_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475430.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGRIIPYGVMAVVIIIFFDYIYRVSKMTRKK"
     gene            complement(14637..16031)
                     /locus_tag="PC523_001906"
     CDS             complement(14637..16031)
                     /locus_tag="PC523_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDF2475431.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWRIDKELIGLIMVGI
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(16157..17410)
                     /locus_tag="PC523_001907"
     CDS             complement(16157..17410)
                     /locus_tag="PC523_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2475432.1"
                     /translation="MENKSNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALSLS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGALGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGLLLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKSLNNKIA"
     gene            complement(17461..18615)
                     /locus_tag="PC523_001908"
     CDS             complement(17461..18615)
                     /locus_tag="PC523_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2475433.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKDAMKPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEEKVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
     gene            complement(19176..19748)
                     /locus_tag="PC523_001909"
     CDS             complement(19176..19748)
                     /locus_tag="PC523_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2475434.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFARKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSIQ"
     gene            19951..20178
                     /locus_tag="PC523_001910"
     CDS             19951..20178
                     /locus_tag="PC523_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2475435.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(20398..21141)
                     /locus_tag="PC523_001911"
     CDS             complement(20398..21141)
                     /locus_tag="PC523_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2475436.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEQEYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILSLMGFANNFGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDSGIVIYFGVDEIAPESFGIPKFKLKFSD
                     FGNFINSRFYCISPDIYVQSRGRVKYFMG"
     gene            21308..22180
                     /locus_tag="PC523_001912"
     CDS             21308..22180
                     /locus_tag="PC523_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2475437.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGNYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGYIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            22180..22845
                     /locus_tag="PC523_001913"
     CDS             22180..22845
                     /locus_tag="PC523_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2475438.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKNHKDAKEAELNYNVLNFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEIYHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            22866..23723
                     /locus_tag="PC523_001914"
     CDS             22866..23723
                     /locus_tag="PC523_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2475439.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIGLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSAKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKYITEISAGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(23860..26406)
                     /locus_tag="PC523_001915"
     CDS             complement(23860..26406)
                     /locus_tag="PC523_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2475440.1"
                     /translation="MNIFNKVALQGLKKNRTRTFVTIIGVVLSAAMITAVTTFGVSLM
                     NYMINGAIIKYGDWHVEFLDVDSSFVQEQRVNDEVENTVTFENIGYATLEGSRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSGKVATDGGVKFAVGDTISLDVGKR
                     MSENENLGQSDPYTAGSEAFIPQNKRTYTVVGICRKPVFEESSAPGYTLITKEDEQVK
                     ADSLSLFVTLKNPRHVRSYTSSAREHDYIFNDNVLRFMGISDNNLFNMFLYSVGGVVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGVIGIPIG
                     VVIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIVVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQQIYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVKDLKQMSNQRSEVTDQDIIFSTQGMKDSEMLKLYDKLKTASGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYNGLHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPTEEYTGQNAKMIAVSMLTKDNHIIDIFTNSSMDVSLTPEVNGKLDTPERQNVNIS
                     FVKVVPPDSPPGIRNSDMKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     SEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWVSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(26406..27092)
                     /locus_tag="PC523_001916"
     CDS             complement(26406..27092)
                     /locus_tag="PC523_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2475441.1"
                     /translation="MAFLKIENLCKVYGKNENQVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(27175..28164)
                     /locus_tag="PC523_001917"
     CDS             complement(27175..28164)
                     /locus_tag="PC523_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2475442.1"
                     /translation="MFRNREFRRFAILFLLITILTVALGFAISTMTGIISIVSATTFG
                     TAFFVFTKNRYKSIAQISEQINLVLHNANHLYITESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIQ
                     MDWLITSLLKLSRLDAGIVVFQKEEIDVDNLISSALHQLLIPMELHNITLHINIPKGV
                     KILGDLNWLSEAIQNIFKNCMESVGDNGKIDIVCEDNFLFTQLTIHDNGEGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAENHPQGGAIFVIRFPK"
     gene            complement(28151..28837)
                     /locus_tag="PC523_001918"
     CDS             complement(28151..28837)
                     /locus_tag="PC523_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2475443.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKENQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(28834..28971)
                     /locus_tag="PC523_001919"
     CDS             complement(28834..28971)
                     /locus_tag="PC523_001919"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475444.1"
                     /translation="MEVLCIDCIRTKETNENCVIISKIVVYYYSAGIYKTNKIKEDES
                     K"
     gene            29255..29584
                     /locus_tag="PC523_001920"
     CDS             29255..29584
                     /locus_tag="PC523_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2475445.1"
                     /translation="MDLNKEVLKGHIDTLILSILNKGDSYGYEIAKIVINETTFELKE
                     STLYVSLKRLESKGLIKSYWGSDQGLGSRRKYYNITDEGKENLELKIQEWNFIQSVMN
                     KFLKRGE"
     gene            29589..30038
                     /locus_tag="PC523_001921"
     CDS             29589..30038
                     /locus_tag="PC523_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475446.1"
                     /translation="MKIIEEYLEALYKNDTSKEIQDLKEELKEHLIDSTNEFIENGYD
                     INEAQNKAIERFDGSNGVSVELRSIFKQKIEPKKVLLRKLSNSRIVFLNLFGWLLAAT
                     AYTINRGFGHIAPPWIIILLIISIVILIILTIIIKGLKKEIENDYNI"
     gene            30105..30482
                     /locus_tag="PC523_001922"
     CDS             30105..30482
                     /locus_tag="PC523_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDF2475447.1"
                     /translation="MVKKILLEKFLDTSGVSYINEKLNLFKDTKWISFGTYIMTVDSF
                     CYREDSKHYNPIMSTFDYESEIFIVNALYKENIYSIKSKKLYRNVSKGDKIIVTIKYS
                     YDKYENLIHEEIHDYHLYPLSLD"
     gene            complement(30667..>31053)
                     /locus_tag="PC523_001923"
     CDS             complement(30667..>31053)
                     /locus_tag="PC523_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDF2475448.1"
                     /translation="VVLATVNNHDKDEFIEIAKDMKELGYTFVATEGTAKSLRENGIE
                     ADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRTAIEFSTEIMTSL
                     DTLKALVEVKKKHLNKDKLKVYNIAE"
BASE COUNT        10228 a         5562 c         3588 g        11675 t
ORIGIN      
        1 actttgctta ttaatttaat attatacttt cagttttagt atttaatcca tatcagataa
       61 taatcttaaa atgaccaaag tcttaaaact atttatcttt atcagattta tcttctaaat
      121 ttttatttat atcaataaca ttgtttcttt cttcttcatc tttttctaat tttatagtta
      181 attgctcttc aacttcttta gaagaatttt cctctgtaac tgcattttca atttcattca
      241 gaggtaactc tttattaaat gcttttacaa attgttctgc atctagagtt tcatatataa
      301 gtaaagcttg tgccacatat tctaatctat ccatattttc ttgtaatata cttcttgtct
      361 tatcatatgc accatcaact atacgttttg tttcctggtc tatctcaaaa gcaacttctt
      421 cagaataatt tctcttactt gaaaaactat tacctaggaa tacttcatca tcactgtcaa
      481 atgacattgg accaagttta gaactcattc catactttgt taccatactt cttgcagtag
      541 ctgtaactct ttctaagtca tttgaagcac ctgtagatac atccttaagt actagctctt
      601 ctgctactct accaccaagt aaaactacaa tattttcttt catttcattt tttgtagcat
      661 agaacttatc ttcaacagga agttgcattg taaaccctcc agctcttcct cttggaacta
      721 ttgtaacttg atgaacagga cttacttctt caagtacatg ggcacaaact gcatgccctc
      781 cttcatggta tgctgtaagt cttctttctt tctcacttat tactttagat tttttagcca
      841 cacctgctat aacctttgtt atagcttctt ctatagtttc catcttaatt ttcttttctc
      901 tttttctagc ggttaatatt gcagcttcat tcattaaatt ctctatatca gcaggagtaa
      961 atcctggtgt tcttctagcc aagacatcca tttttacatc atcacttaat ggcttatttc
     1021 ttgagtgaac tttaaatata gcttctctac cttttacatc tggtgtacca acaacaactt
     1081 gtctgtcaaa tcttcctggt ctaagtaacg ctgggtcaag tatatctggt ctatttgtag
     1141 ctgccataat tattatacct tgatttactc caaatccatc catttcaact aggagttgat
     1201 taagagtttg ctctctttca tcatgacctc caccaagacc tgcacctctt tttctaccaa
     1261 ctgcatcaat ttcatctatg aatatgatag ctggagcact tttcttagcc tgttcaaata
     1321 aatctcttac tcttgaagca ccaaccccaa caaacatctc aacgaaatca gaaccactta
     1381 tactgaaaaa aggaactcct gcttctcctg caactgctct tgatagatat gttttacctg
     1441 ttccaggagg ccctaccata agcattcctt ttggtattct tgcacccagt tcaatatatt
     1501 tcttaggatt ctttaagaaa tctactacct cttgtaaatc ttctttttcc tcatctagtc
     1561 cagctacatc tttaaatgta actctagttt tttcatcatc tttatgaacc ttagcttttg
     1621 atttaccaaa gctcataacc tttccgcctc caccttgaga ctgattcata aacacgaacc
     1681 aaataatcac cataaagaat attaacagta atgtaggcaa catttcgaca aaccaaggtg
     1741 tagatggctt agcttcacca ccaaaagtta gtttcccagc cttagcttgg tctaacacct
     1801 catctgcaag tttatttccc atcacttctg ttggaacata cgacttaaac tttgtatttg
     1861 tatcttttat agtaccttct acagaagttt gatttacaaa gtataatctt gatatattct
     1921 catctgttaa ttctctatat acttcagaga attttaaatc ttttattttt tctgtaggct
     1981 tacctgagaa ttgaactata cctactataa ttataaaaac aagtagataa aaacctgccc
     2041 cttttagcaa cttattcaat atagaagccc tcctttcata cattgctttg ccctataatt
     2101 tgttaaaatt aatttttaat acttcttttg tgttttcatc aattttataa ttttcactga
     2161 ttttataatc tccaacacat attattcctt cactatctgt gattatagga attttacatc
     2221 tttcttctct tgggattttt aaatctatga ataaatcttt cactttttta cttcccatag
     2281 caagctttat cttatcacct tgccttctac tccttattac tataccccct tttaccttat
     2341 taaaatcaaa ccctttagat gaattgtcta atttcatact cttataccta tctatactca
     2401 ttacttgggt ctcaataatt tgttttaatt cttttatttt aataaaccca ttactaggta
     2461 tattgtaata aaattcaatt tcctcactaa caatttcctc attcgttaat attatactgt
     2521 ctttttttct ataaacaaat atatttcttg gaagagttaa cattttattt acttttgaat
     2581 tatcttctaa agacattata tcctctatat gtctttggtc aacaaaatta gtatctccta
     2641 gtatatgttt tatagaattt ctaagaaccc taactttgat agcatcatgt aagcaaacaa
     2701 aatcgtctaa atttatctct acaaagcctt tttcttttat atttgaaact tctctaaatt
     2761 tagcttctgc ctctttctca atataatcgt tatcactttt taaactattg ctcatcctca
     2821 ctatagattc tattacattt gaattaaaat tatccttcat atatggtaaa aggtctaacc
     2881 ttatcttatt tctagtgtat atattttcta aatttgtttt atctatcctc ggatttaaat
     2941 tataagcttc acaatactcc tctatctcac ttctttccac atctaatatt ggtctaatta
     3001 tgcaattatc tctaatatag tcaattccct ttaaaccttt taaacctgtt cctctcatga
     3061 tacgcatcat aacagtttcg gcttggtcat tgagattatg acctattgct attttattag
     3121 ctttaatctt atctttgatt tcataaaaca tttcatatct tagcttcctt gctccttctt
     3181 ctaatgataa tccctcattt tcacaatatt ttggtacatt aatagacttt acaaagaata
     3241 taattcccat atcctcacaa agtttagata catataatgc atctttttga gcttctatcc
     3301 ctcttatttg atggtttaaa tgtgctgcat atatttctat attaaaatct ttttttaatc
     3361 tatttagtac atgcaataaa caaactgaat caggccctcc agaaagacct aaaactattt
     3421 tatcgccttt ttgtattaaa ttatgtttat ttatagtact tagtacctta tcaaatatca
     3481 tatttatccc tcttacactt agttttattc acttttaagt atactttcta taaaaaaacg
     3541 ctaaaccctt gtatcaattt taagaaatta aagtttaata taaatataat aataccaaat
     3601 aaattaaata aaaggtagtt ttttcactac ctttataaaa tttctggaaa atagctatag
     3661 ttattattta gtatctatgt atatatctat tttattccta cctaaaaata aatcttgtac
     3721 atttatatta tagtctattt tcaattttat gtgttcccct tcttgtatat ttatatccaa
     3781 ttttcttgta tcaatgtcaa ttaataaagt accttgtggg gttacatact ttgtggtact
     3841 actctctcca catttaaaca gcattgttga gttaatagca ccaaattttt tgatactaac
     3901 ttcattttta agtatcttta tagtattagt tatttctata ttttcttcct tttccttgta
     3961 aacaacatat attccatcat ttttataaaa aattttacca aaggcagtca tctctatggt
     4021 gtccatattt cctttttcat catattgtct agtatttata cttattttta cactcaacga
     4081 tttttcctcc atagattctt catataaact agtatttttg tatttctact ttttgaatcc
     4141 tatcaatttt tttctttaag aactctcttg ctatatctgt aaatttttca ggtatatctg
     4201 atacataata ttgataagtt ccatgaattt ctgtattgtt tataatattt ttcacctcta
     4261 aaaccttttt taaatccttt gcagtctctt tggctggatt aacaagtttt atatgctctc
     4321 caacttcttc gcctattgtt ctctttaaaa taggatagtg agtacatcct aacactaaag
     4381 aatctatatt tttttctttc aattcttgta aatatatctt tgctgtcaat ttagctactt
     4441 ctgtatttgc ccatccttct tcgacaatag gtacaaataa agggcatgct ttattaacaa
     4501 tttctatact ctcatctatt ttagatattt ccaaattgta tgcttttgaa cttatagttc
     4561 cttctgttcc tattattcct acaattttgt tttttgtaga acttacagct gttctagctc
     4621 ctgcttctat aacaccaatt atcggtatat catacttttc ttgagcttct tttaaacttc
     4681 ttgcagtggc agtattacat gctataacta tagcttttac accttttgtt tttaaaaagt
     4741 ttatagcttg aaaggtatat ttaattatag tttccttaga tcttgaacca tatggtattc
     4801 tagcagtatc tccaaaatat attatatcct catttggtaa tatcttcatt atctctttta
     4861 aaactgttaa tccacctaat cccgaatcaa aaactcctat tggtttattg ctcatcttta
     4921 tacaacacct actttacatt tttgtgttta acaacaactt atttatcact aaaatattta
     4981 tttatattca taattaatat attattacct aaatgattat tatagtttat tttacacaaa
     5041 aaataaagga gctatcttaa attagaaaat attttctaga aaaagacaac tcctttcact
     5101 acttaaaact ttaaatttat tttgcaaaca catgatttcc tattctcata taaggattca
     5161 atctatttat ccatttgcaa gtggctattt ctggattcca gaaatatact gctttattag
     5221 ttgggtcagt tccataaaga gcttcttgag ctgctttacg tgctgagtct gttggtgcca
     5281 tctttattga accatcaagc acaactgaga aagcattttt ttgatatata acctcttcta
     5341 ttgtgtctgg aaatctaggg tccaatactc tatttattac tacagctgct acagcaacct
     5401 gtccctcata gctttctcct ctagcttctc ctgcaaccaa cttagataat aacatcaagt
     5461 cattatcaga taaatttatt acttctttct ttgctatatt tttttcttgt ttgtttattt
     5521 tctcaatctt tgctgttggt tcatatgcaa atacctcttt aataggcata aatataaaag
     5581 tcaaagctaa tagtgtagcc atcagtttac tccacctgtc catgattaaa accactcctt
     5641 acattcttat ttttaaaatt ttttctctaa atccttcact acttcaatta ctttaaattt
     5701 aatttttact ctacttgttt tttttggtgt atcttgtttt attaacttat attctttatc
     5761 agttagaact tccttcaact tatcatctgt agatttatct attactccat tagattcatc
     5821 aacaaagcat agtggaggga acataacaca ccaccaattt tttccttcac cttttccaat
     5881 gataatcttt aatgccttat attcaccagc tggaagaact atattagaat attgtttagt
     5941 aggaaaattg ctatattgta tacctacctt tacactgtaa ttatatccat tttcaagaat
     6001 aatattttta cttatttctt ccagactact atattccttt tttataatag cttcactttc
     6061 ttttatactt tttgaatttt gaagctttgg ttgtaaatat tttataactt catctctaac
     6121 ctttagttta agttcttggt cttcatctgt attactattt gctataacat gaaatcttat
     6181 taatttatct ttgtaatcct ttgatatatt gtctactttt tttacctcac catttattac
     6241 tattgtcatt attgatatga ctgatataag acttaatatt aatagtccca atctaatctt
     6301 aatttttctc attgaaaatt ccccctttaa gataaattta tatcatcaat tatgtccaat
     6361 taaatttaat ttattcataa tattttttaa tactaataaa aaaagctatc ttaaaatgat
     6421 tacaatcatt ttaaaatagc tttttaaacc ttatatttta taagtcttta ctaatcatag
     6481 ccttatctat cataaactgc tctgcttttt ctatatgctc tgtagcatac ttcttggata
     6541 gctcactatc acctttaatt atagcatcaa gtaaatattt atgctcttca acaatattat
     6601 gtgatgtatc ataagcagac atataggtaa ctctaaacct atatacttgt tcccataatg
     6661 agtttattag ttggattaat tttttattat tagttgcttt aaatatacat tcatgaaatt
     6721 ctacatcaag ttttaataac gcatctacat cggattccaa agtgcttttt ttaaattctt
     6781 ctgatatatc tttaagcttt ttaatatctt cttcactaat tctttcagcg gcaagatatg
     6841 ctgctaagcc ttctaggctt gctcttactt ctaatacatc cataatatcc tttaatgaca
     6901 tatttgccac atacgcacct tttctaggca acataacgac taaaccttct aattcaagct
     6961 ttcttattgc ctctctcact ggagtcctac ttactcccaa ttgttctgct agttgcactt
     7021 ccattagcct ttgaccaggc tttaattttc cttctaagat tgcttctctt aaattctcaa
     7081 agactacatc tcttaatggc ttgtaattat ccaagtttag tttagttaaa ttctccacaa
     7141 atttcaaccc ctttttgact gctattaaca acatacacct gtttatattt ttttaataat
     7201 tctttttttc caattagagc atcttcttta tttttaaata gtccaaaaac tgtaggacca
     7261 cttccactca tcatagaacc taaagcatta tttttcatca ttatttgttt tatatcactt
     7321 atttctttgt gtttacttat agtaacattt tcaagaatat ttaccatgct ttctgataca
     7381 gcttttatat cttcagattt taaacactct atcaaatatt tattatttgg tcttttttta
     7441 atattattca aatctaatcc ttgataaact tctttagtag aaacgaataa gtctggtttg
     7501 catatcaata tatttgtatc acatggtaaa cctttgatgc ttgttagctt ttctccaata
     7561 ccttgagcta gggcaggtct tcctgatata caaaaaggaa catctgcgcc taaattaagt
     7621 cctatttctt ttaattcatc ttcagataat ctaagtttcc atagttgatt taaacctact
     7681 aatactgcgg ctgcattaga acttcctcca gccattcctg cagctacagg aatatttttt
     7741 tctatgaata tctctacacc cttttttata taaaatttat ttttaagtat tttggcagct
     7801 ttataaacaa tattatcttc atctaaagga atatctaaac tagtgctttt tacctttatt
     7861 tcatcttctt ctaattcttt tatctttact atatcgtata aatctatcgt ttgcattatc
     7921 atttcaacaa aatgataacc gtcttgcctt tttcctaaga catcaatgga taaatttatt
     7981 tttgctctac tctttagtct tataaaatcc attctatcct ccttgtttaa agaataatgc
     8041 atcactaaca ataatattat actacaaaaa ataaaataat agggtattat aataccctat
     8101 tatacttaat tactttaaat tttttaatat taatctacta atacgacttt tttttctaaa
     8161 ataagacact taccaggctt aattggttca tcaagtttta tatcattaag ctctgcaatt
     8221 tcgttttctg ttgtattgta tttttttgct atattccaaa aagtatctcc ttctttgcat
     8281 atatatacga ttatgctagg agctttagat aaatcataca caccttggtc ttcacccttg
     8341 ataataaaat tttctgcttt cttatctaat gcttcagtaa atctcttaat tttaataatc
     8401 aagtctatct ggtccctatt caaatcaact tcaactttat caatacatgc tgtattaaac
     8461 acagatgctg tatcagttag attatccatt tctatatcat gttcaaaagg aatttcctca
     8521 ctaattttat aaaccagttt taaaccttca actggtacaa ataaaatttc aacttttatg
     8581 attccttgta taatgctttt atttccttca atataactat tttctataga catggttgga
     8641 caaacgctta caatatcttt tatttgtata tcatcattat catttcttat gccttctcta
     8701 accataaatg tttcttcact acgtcttaaa gtcttattta attgaatagg tttatggtca
     8761 aattttatta ttttttgagg cgaatatgca tcttgtaata cttctcttgt aacttcatct
     8821 gtaactttta ccttacaaca agccatacaa tctatttcta aaagacctgt gttactttca
     8881 ctattttgct taaaaatatg attaaaatct gacatagaca acaatacttc ttcagtcata
     8941 ccatcactaa ccccaggtac ttctacaaat tgggtaaact caatacctac tctatctaac
     9001 tctacaagtt caccctcgta tgtacaagct aatggattta tttctaatac tccaccaata
     9061 ataactttat tgtcagttac tctactttct tttactttaa cacatggatt taaacttatt
     9121 atagattgaa tttcctctgt gtttattgtt atagtatctc ttatagaact ttctgccttt
     9181 tctattccaa ctatatcttg aaaacaaatt tcctttctgt gcttttgtat gccttctacc
     9241 tgtgccacat cttttacaat gtctagcctt tgcttttcaa acaaacttcc ccttatattc
     9301 ataagagctc ctactttaat ttttctctca ttcattatag tacaatccat atgctctact
     9361 tctgaaaata acatgtattc catatcttga acaacattgt ctttttctat tacttcgttt
     9421 atatctactt ttccatctac attagaaata gtatttttat catctgcaat atagattaca
     9481 ttataattaa aacttcctct gcaaagtatc ttcccatctg ccatttcaat ctttttaagt
     9541 gatatgtatc cttctgtttt tacaatttca tatacatcta attttttgtc tggtacaact
     9601 gcctccgctt cgataaatgt ttgaaatttt ccaaagtcta ttctattgtc aaccttaatt
     9661 acatctttaa ttaattccat agtctacctc cttaagtatt ataaaaatat cacactatat
     9721 ttatatttaa ggagcaaaaa aaaaattaca cctattttta ataggtataa ttttaactta
     9781 tctgtaattt atcttgatct ctaaaaactt gcaactttac attagatgtt aataaatctg
     9841 agtaactata agatacccta ggatatccat tactttcatc atctaatttt ataacaaaca
     9901 cgcttggata aactttttct agtataccct cttttgtaat aatctgcttt cttcctttat
     9961 tagctttcaa caatattttc tttcctatat gtctctctaa gcttactctt atcttatcta
    10021 gagtttgaac agtagccaca atatcacctt ctttaacgtt tatatgttta cataatatca
    10081 catataaaca tttttgtcaa atgtttaaac taattatttt atatattttt tgcaaaaatg
    10141 tcaatactat ttttaaagat atacagtata attatgctaa tttttgctaa tgtaatattg
    10201 tttttggcat tgcccatcta aattttcctc ttgtttcaga accaccaatc cccttaactc
    10261 ctgtaatagt ctgctctata acctcatcaa taggcactac tacatcaatt cttgaacaag
    10321 caattttctc cactataaaa acagggtcta atgcatgtat cattattctt ccaggcgaac
    10381 ttgcatagtt tgctcctgca cttattattt tttcataatt tgattggcat gctcctgcaa
    10441 atatgacaag attatccaaa tttggttgcc atttgcgtgc ttcttttact gttttaacaa
    10501 aatttgaaga gtttctatag ttactcatat cttctatatc tcctcttttt attgtcattg
    10561 catcatgacc tgttattact agtatatccg gattatattt ttctagtagt gctcttactt
    10621 ctttatactg attttgttct gaaacagcta cacctactgc aggtattcca agttgagcat
    10681 atacatccaa gcaaatcttt aaatattctt tatctccatc tatctgtaat acctttccag
    10741 gcattccata tgtgttagga tttaattgaa gttttggaac tgctcttgtc attctttttt
    10801 gtctttcctt agcctttctt actgacttat ataataaatt ttctacattt ttatctatta
    10861 atatatcttt tatatcaggt gcttttacta attctaggtc atcaatataa gcatctgcta
    10921 ttactctaaa tgcaattccc tttagtatag ctattttctc attattttca tctacaccaa
    10981 aagagactat tttaaatact atgtctttgt tatgtgactt tctagctaca atgtctccta
    11041 ccttcatagt taaacctcca ataaattttt atcttagttt atactatgtt cgatataaaa
    11101 ctttgtttcc taatgtcaaa aaaacatttc ttataatact tcttttatta ctcttaaagc
    11161 atttttatag tatatctttt ctatctcatc ttcactaaac ccttcttttt ttagaggttc
    11221 atacaattta cccatctgag atatatcttc aatctcaact tttgaatcta ttccatcaaa
    11281 atctgaacct agagatacaa catctattcc tccaacattt acaatatgct ttatgtgtct
    11341 aaccatatct tcaagtttac tttcgctttt atcacttaaa aataagtgga agaagtttat
    11401 acctgtaact ccacctttat ttgctaatac cttaatcata tcatcagtta aatttctaga
    11461 atgattcatc attgctcttg aattagaatg tgtagcaatt attggtttag aagaaatctt
    11521 cgcaatttca taaaatccac catctgaaat atgagaaaca tcaactaaca ttcccaattc
    11581 attcatctta tgtactactt ctctaccaaa atcagtaagc cctttttctc tatattctaa
    11641 tttattatga ggaaaactca attcattcac atgattccaa cttattgtca tcattctaac
    11701 tcctaagtcg taaaatttat ttaagttttc taatttacct tcaagcactg caccttcctc
    11761 aatagataaa agagctgtaa gtttaccttc tgattggttt ttcataattt cttcatagtt
    11821 agtagctaaa gctatttcat cactattttt tttcatttcc tcatgaaatt tagttgccat
    11881 actcatgcaa taatcaaaag gatgttttac ttcttcagta tcaacaaaaa gagcaaatgt
    11941 ttgagctaat gaatccccct tttttaatct gtctatatca acagaatatt tattagactt
    12001 aagctcactt gtttctacat tttccattaa ttttgctata gtatcacaat gtaaatcaat
    12061 aaatttcata tttatacccc ctgtatattt tatatttata tctaaaaata gaaatagttg
    12121 tccttataga caactattga caactatttt ttaatattct ttttgatatt acttaatcct
    12181 attcttcttt taagttaggt attacttttt taacttcttc aatttgatgc catgttttat
    12241 ctatattatt ttcactaata tattttttta aatcagttaa agatttaaaa tctttaactg
    12301 ataaattgtc atcatcatct gcaaatctta aaataaacat gagactacct tactttccac
    12361 aacatttctt atattttttc ccacttccac atgggcatga atcatttctt cctatttttg
    12421 cttctttaac tattgtttta gacttcttat aagcctttat tatttctttt ctttggtctg
    12481 ctggtagtat atcttcccaa ccttgtaaat tatatagcca atgggcttca acagttacca
    12541 tattgttata taatttttcc caatctattt ttatagaaac ttctttatca gcttctacat
    12601 cttctaaaac tatatcttct tttaaacttt cacttattcc atctatgaat cccatgaaaa
    12661 attcattagt agttccaaat ttttcggcta attcgcttac tttaccttca acaacatcca
    12721 ttttattatt taatatttca ttgtatattc cagcttcaac tttcaaatat tcttcccaga
    12781 attttacttc tgcttcttga ttttcatgat tatcacttaa atttctccat tcagtatata
    12841 aactcatact ttttcctcct aagtatacct atatttttat agtttttata gtataaaatc
    12901 ttttagttag attggaagtt tcttcttttt gattatacca tattctattt aaagttaact
    12961 cttatattat atcataatta aacaattctt ttaatacttt tatagcatac tcttggtcta
    13021 taaaataagg gcttgtcgca attgttatta cttttgcttt tttaattaaa ttttttatct
    13081 tgtaaaattt gagttcaaat ggtatatagt ccatatcact tgaaaatata tccaaatcta
    13141 tatcaagaac aaactctgat tctacatcta aaccaaatcc atataggcta tctattatta
    13201 ttaattctga aaatatgtta tattttaagg caggttttat aaaacttccc acatttaaaa
    13261 cttcatttgt atacctaaat acatctttta tgttatttat atctacatta tagttttcag
    13321 gttctctagt atccttatgt tggtctacat gcactaactt acatcctttt gtaaactcac
    13381 cctttaacaa agattttatc caaaaataaa atgcatggtt atgattatca aatacatata
    13441 tctttttatt tcccaggaca tattcgacca tattctccaa tccttttgct tttatttcct
    13501 tattttcatc tacttcatta aagactacct cattgcccaa tcttacatta tttaatgtac
    13561 catttataag ttttggaaca tatatactct tattttctct tttatcatac gaaaatacgt
    13621 tatttcccac cggtttttct atataaaatc cactatattc ataattttcc ataaaacttt
    13681 acttcctcca ttgttataat atataatcta taattttaaa aattaaaatg cacttattgt
    13741 aaatcatatt atctactata gaaaacatct tatataacta aatataatat cttctctcaa
    13801 tatgtattat acaataagtg caaaatatat ctatataaaa tatactacat tcctataaca
    13861 aaaccatcag cttcaattgg aaggtaacta actcttctat tcaactccac ttttccaaaa
    13921 aatactaccc aatatggtct aaaaatactt tcaattttga ctatttcaat ctctgggatt
    13981 ccacccataa acctatgagt tagtctcata gctattttat ttgcctttgc ttttactctt
    14041 cgttcatcct tatcactata ttgcacaata ttacaatcca cattttttat aatttcatat
    14101 gtaggaaaat cttgtactat actaccagaa cctgtagagc cattaactaa aattttcaat
    14161 tcattcacac cattagaata tctcctactc ttgaaaatgg atttagtttt gtgaatttta
    14221 agtgtgacaa ttttaaactc tatataatga agtctaactt catgagcata tttattcttt
    14281 aatattagct tactaaataa agaatttttt ccacttttag ctatatctat agcttcttct
    14341 tttgtaattt ttgtaggtaa tgcccttata tccataaaat cattctcttt cttatttttt
    14401 tcttgtcatt tttgaaactc tatatatata atcaaaaaat attataatta caactgccat
    14461 aactccatat ggaattattc ttccaactat aatatcattt ctagtcaata taccataaaa
    14521 tataaatcct gttattaaaa agtacaataa tactagaaaa ccatctattc caagccattt
    14581 tatttctaaa tcttttttca ccttagatac taatttagac atagcagttt ctccttttat
    14641 agattatttt gtgtatttaa attttcccct attctattgc caatcatcca tgcaataaat
    14701 gaaaaaggta tagctatcac aagaggttct aactcaaaac tctgaactgg gaatatttta
    14761 aaataaccaa aagtcactcc acctacaatc attcctaaaa taccacccat agatgtcctt
    14821 cttccatttc caagaagtcc acctactata ggtacaaatg ctgaacccat ccaaatactt
    14881 cctacaaata tacatgcatc atatacattt tcaaatctaa atgctacact aagacccata
    14941 actgctgcta agcatacacc tattctagta aactttacta attgtttctg agataactct
    15001 ttttttgtaa ttcttccata tatatcattt gctaatgtag ctccagctgc tagataatag
    15061 ctatctaatg ttgatattat agaacctaaa attcctatta taaatattac tcttattcct
    15121 tgtggtaatg tatctagaac taaagttatg tatccagcgg ctggagccat ttctggataa
    15181 gtagctctta ctataagtcc tgtcatagtt aacaccatgt caaaacatat ccatagtgaa
    15241 aaacaagtta ataaagctct tcttccaacc ttaggagaat ctgctgctga aaatctttga
    15301 taaaaagttg ggtctgaata agctccaaaa gaatatacaa ttaacatcaa tgctttcata
    15361 gctgacatac cttttgtagg gtgcataagc tgtggtgcat ctttgagtgc ttctgtgaga
    15421 ccttgaaatc ctcctatttg ttcaaattgg cctggcataa ccatagttaa accaaatacc
    15481 atacagaaaa acaataccat atctgtaatg gcaactccca taagtccacc aagtactgtg
    15541 agtacaacaa ctattcctac cattattaag cctattaatt ctttatctat tctccatgct
    15601 acgttgccta tagttcccat agcagttatt tctccaagtt gtgcacaata tataaacatc
    15661 aaaattgccc caattatagc aacatgcttg ccataaaaac tttccagtaa atcaggaact
    15721 gttatattgg ttcttatatg aatccttgga ccaacccaca atgctaaagg cattctccca
    15781 atatgtgcag ctattgacca aactgcccat gcagatattc catatatagc tgcatactct
    15841 acactaccta ctgtaccaac tcctccatac catgatgcaa ccaaagttcc tactatcaag
    15901 gaccatggta aacttcgatt tcctaggtaa taactttcca tatcttttac atttctagaa
    15961 aaataataac ctattcccaa taatataaat gcaaatataa ctataatccc attctcccaa
    16021 aatgttaaca tatctaatct ccacattctt atattttaat ttttaacttt aatatctaaa
    16081 tatgaacaac tctacccatt tccataaaaa taggtagagt tatataataa ttataagtat
    16141 ttaaacaatc ttgttattaa gcaattttat tgttcaagct cttattttct cttttattta
    16201 tcttatttaa agcataagta gcaaatatac ctataactgc acatactaca gataaaataa
    16261 atatatatgt atatcctgtt tgtccatatt tatctaacca attacctgca atagtgtaat
    16321 aaaatgcatc tggagaaaac cctataaatg aagcaaatcc aactactgct cctgtagttt
    16381 ctaaagatat attagactct gaaacagatg caaagtatat acctctaaat ccaaataaaa
    16441 ttacacttag tattatcatg tttattacag ctatccatat aaatcctgta ctcttaggaa
    16501 taaccaagta agcagctgta cttactgtca ttagtataaa tcctacaaac attactctta
    16561 tagatgaccc aactttatct gttattacac cagcaactgg agatgctccc atttttataa
    16621 cataagttct aattattgat agagcagaaa ctagagtcat tggcattaca taaacttctg
    16681 ataggtatgg acttaaataa gttaaacttg agaatactaa ataagttgaa aatattataa
    16741 gaccaattag ccaagccttt ggcattgtaa ctacctttag caatgactta atgcttaatc
    16801 cttcatctga tgctccacta tcttttttct cttctactaa gaaaaataga agtaaaccac
    16861 atattataga tgctgcagaa tataaccata ctacatattt gaatcctact gttatttcag
    16921 caaatttacc aaagaaataa agacctataa aagaaattaa tgtacctgat acacctgata
    16981 atccttcata aaaaccaaat attcttcctt gctctgactc acttccttgc attctaacaa
    17041 ccttaacaga tgctgagaaa aaagttaata tggttgtaaa tccccataat acaaatatta
    17101 aaagtatcat actatatgat gggaatgtag aaaaataaaa cccaagcgct cctgaagcaa
    17161 ttaaagaaaa agttatcagc tttctaactg agaatctatc tgcaaaccat cctcctataa
    17221 aataagatac cattcctact aatccatata aacttaataa gttccctatt tgtgtatgac
    17281 tcaacgataa agcttgttgc attgggtcat aaaatgatga tttcatatat ggtaaactat
    17341 acatagctgt tgttccaaat gccaatatga atattatcaa aaatctttta acattacttt
    17401 tgttttccat tgatactctc tccttaaaat tgtattgttt ataatctcat tcatgtaatt
    17461 ttattttaat ttttctattg cttgtttaaa gtcatttatt atatcatttg aagattccaa
    17521 ccctacagct attcttatta gattttctcc attcatttga accaatgtat gctcttcccc
    17581 taagcttgtt gctattgtcg caagctctaa agagttcatt aatcttctac tagcttggtc
    17641 tcttgttaat ccattgatac catccttaag cttaaatgaa actacacctc cacctaaacc
    17701 attcatttga gttttacaaa gctcgtattg aggatgagat tttaatgctg gatattttac
    17761 taattccacc ttctcttctt cctctaaaaa ctctgctact gctaatccat tttcacaatg
    17821 ctttttcatc ctcatatcta acgttttcat acctcttaaa atcatccatg cactcattgg
    17881 tggtaaagac gctcctgtaa aacaacataa acttggccat ctaactaaat ctattaattc
    17941 ttttgaacct gctactgctc cccctaaagt atccccatgt ccatttataa atttagtaag
    18001 actatgtatt actaaatctg ctcctaaatc tattggtctt tgcacaattg gtgtaccaaa
    18061 agtactatca actatggcta agcttttatt ttcatgagca atttctgcta ttgtttttat
    18121 atctactaaa tctaatgatg gatttaaagg actttctata taaactatct tagtatttgg
    18181 tttcattgca tcttttacat tttgtggatt tactgcatct acaaatgtaa cttcaactcc
    18241 aaatttagat aacaactcag acataaataa tttagtgtgt gagaatattc catctgaact
    18301 aactatatgg tcaccacttt ttactaatgc taatatagta gatgttattg cccccatacc
    18361 tgacgtacta gatatagcac tttctgcatt ttcaagcata gccaacttag cttctaactc
    18421 cgtaagagta ggattacact ctcttgtata cacatatccc catgtcatac acaactcttc
    18481 aaaatgctct actgtctttg cagcaaatgt tgctgtttga catattggtg tagccaatgc
    18541 acctgttgtt gggtctgcac tatgtcccca atgtattatt tttgtctcta aatcttgatt
    18601 aatattgtta ctcattttga tttcctccca ttttgactta tttatacaac gttttcccta
    18661 ccaaatttca tcaatttagt ttagtcttaa tacatttttt caagtatact tttatatttg
    18721 ttataatttc taaaaatatg acacttattt tgtcttttta cagttctaat tataaactct
    18781 atagtaacta tagagtcaat atgtttttga tatttttttt acaatgttta aaatagttgg
    18841 tacaaaaata ggtatatctt agaataatta aagtcatcct aagatatacc tattttcata
    18901 ccataattta aacaattata taaagcatta atttttttac cataaataag tttttcaaga
    18961 gttatcatct tttaataaat atattttagt taaatatttt aatattattt aatctctcca
    19021 acaaaataca ctctaaaaac aaaattatat aattaaaaaa atatcctata aaaagctaaa
    19081 aagtttttct atttactaca actaaaaata agatttgtta gaggtataaa tgtatttagt
    19141 ttatttacac ctctagtatt ttacaaatta tacctctact gaattgattt tttagcacga
    19201 ataagtgtgc tctttgagat accagtcatc ttcacaactt ctctatatgg tttagaattt
    19261 aataatccta atgcatattg tatctgttga ttgctgtatt tctttggcct accatcttta
    19321 aagtcctttt tcttcctagc aaattctttt cctaattgag tacgttctat tatcatattt
    19381 ctctcaagtt ctgcaactgc aagtaacgta gtcaaaaaga acttacccat tgaagtattt
    19441 tctaagagac ctatgtttaa aatatgcaca cttacttctt tagcaaataa accttctatt
    19501 agttgtatcc cttctgatgt acttctagct aatctatcca acttagttgc aacaagtaag
    19561 tctccctttt ttagagatga tataattttt tcaaatttag gcctatttgt agtagtgcct
    19621 gtataagttt cctttattat tttagcatca ttatatttat tcaaaatttc ttgctgttgc
    19681 tgatacaaac tgttgttgtc tgtttgattt tttgtactta cacggcaata tccatatata
    19741 ataatcatca cttcctaaca taaaaatttt gtacagaagt tacccagaag cttgtttttg
    19801 cttatcttta tccattttac taaaacggtt ttttagtgtc aatactgtta acttttgaca
    19861 ccataataat tcctttataa tttgtttgac tatgtcaaaa attaccaata taatgaaatt
    19921 aatgagttta tacaaattgg aggaaataat atgaatagtc tgttaaaaga agcgatagat
    19981 gaattaaaaa acatccattg cggagaagtt tttttattaa aagaattgtt caaaggctat
    20041 gaatggaaca ggttggagat gaacataagg ttgaaattag gaaaactttt tttgtatgaa
    20101 gtggtaacta atcccaattt aaatgtagag gtcatcaata aaaactctgc ttatcagcaa
    20161 atgtataaaa aaaaatagtg acttaaattt atataatagt ataatcttct tattgattca
    20221 aaagttaaaa cttttaatac aaaacaatca ttctatttta gtatttatca atatgtctaa
    20281 atttatataa ttaacacata aaaatagtac ttgtttaaat cacattcttt aagatagata
    20341 aatcttaacc ctactccagc ataaatacta aaatagggct tagaattatc tatattttta
    20401 tcccataaaa tacttaactc ttcctcttga ttgaacatat atatctgggg aaatacaata
    20461 aaaacgtgaa tttataaaat ttccaaagtc agaaaacttc aacttaaact taggtatacc
    20521 aaaactttca ggtgctattt catcaacccc aaaataaatt acaattccac tatctgtaag
    20581 ataaaaagct tggtcttcag gtataactaa ttcaacatta gaataataca tttctctatt
    20641 ttcatttatt tgagatttaa tgtagtcagt aattatatta atataatcaa ctccatctct
    20701 aaaaatatcg ttaagtaaaa gttcttttcc agttgtcaaa tctatattgt aattattcaa
    20761 tgtattgtat ttaggcccaa agttattggc aaatcccata aggcttaata tcatactcag
    20821 tacataattt ttattaaaag taactgcata acttgtagat acccagtaac tttgttttgg
    20881 aagtgaattt tgagaagctg cgttattata ttcttgttct tcttcctgca agccattttt
    20941 aaaattattt acagtcattc ttacagcatt gtttatgtta tttaaaataa ctgggtttac
    21001 atacgtagat tgatttctaa tattgtatat aacaggatac tttacatcat atgaaaaata
    21061 cttacttctt tcaaaatctt cattaaattg tacatataat atattatatg tacaggtatt
    21121 tgtagattga taattttcca ttaaaatcct ccccttttat aaaaaaatca gatacaatat
    21181 aatatgctta aaacagtaat aatgttaata gtaaatgtaa agaatagaaa gtatctaaaa
    21241 cagcaaaaaa tgactaattt tgtggtataa tacataggat tgagagttat tttataggag
    21301 gaaaaatatg ttattattag cagataaatg gaaagattat gaacttatag atatgggaaa
    21361 tggagaaaaa ttagaacgtt ggggcaatta tgtacttaga agaccagacc cacaggttgt
    21421 atggccaatg gaatctgaat gggctttatg gaaaaaccct catgggcact atcacagaag
    21481 caataaaggt ggaggtcaat gggaacacaa aaaaagatat ccagaacaat ggactataaa
    21541 ttataaaaac ttaaagtttc acattaaacc aactggattt aaacatactg gattattccc
    21601 agagcaggca gctaactggg attggatgat agaaaaaata aagaaagcta acagacctat
    21661 aaaagtgttg aatctttttg cttatacagg tggagctact gtagcatgtg catctgctgg
    21721 tgcagaagta tgtcatgttg atgcggcaaa gggtatggtt aattgggcaa aagaaaacat
    21781 agaattatca ggtcttaaaa atcaaacagt aagatttata gtggatgatg ttgtaaagtt
    21841 tgttgaaaga gaaataagaa gaggtaaaaa atatgatgca ataataatgg accctccttc
    21901 ttatggaaga ggtcctaagg gagaagtgtg gcaaatagaa gaaaagttat atagttttgt
    21961 agatttatgt atgggagttt tatcagataa ccctttattt ttcttgatta attcttatac
    22021 aacaggattt tctccaatag ttcttgaaaa tgtattgtct acaacaatag gtaaaaagac
    22081 aaatacagga tatatatatg gtggggaatt aggaatacca gcttccagag atggtaagat
    22141 acttccttgt ggtatatttg gaagatggga gaaaaaataa tgataaaggt catatatgaa
    22201 gataaccatt tattagttgt agaaaaacca gttaacatat tatctcaagg agataatact
    22261 aatgataaag atatggtaaa tcttttaaaa gactatataa aaaaaaagta taacaaacct
    22321 ggaaatgtgt atattggttt aatacatagg ttagatagac cagtgggtgg agttatggtt
    22381 tttgcgaaaa catcaaaggc agcatctaga ttgtctgagc aggtaagaag taagactttc
    22441 aaaaaaactt atctagctgt tgttcatggc aagataaaat ctaactcaga tatactaaag
    22501 gattttttat ataaaaataa aaaaactaat atggtaactg tagtgaaaaa aaaccataag
    22561 gatgccaaag aagctgaact taactataat gtcttaaact ttaaagaaaa tttaagctta
    22621 gttcaagtta atttaaagac tggtcgctct catcagataa gagttcaatt tgcaagcaga
    22681 aaacatccat tgtatggaga ccaaagatac ggcaaggaaa taaataagat tggacagcaa
    22741 atatctcttt ggtccaataa gatagaaatc tatcatccta ctacaaaaga aaaaatggaa
    22801 tttacatgtc atccccctga caaatatcca tggaatatat tttaagtctc aaatgaggtg
    22861 attatatgag caaaaaattt tcaataggag aaatgtccaa gcttcataaa ataagcatac
    22921 agacactaag atactatgac caaataggtt tatttaaacc tgttcatgtt gaccaagaaa
    22981 gcaattatag atattactca attgaccaat tttcacaatt agatattata aaatatttaa
    23041 aatatttagg tatgccttta aaagaaatta aatcaaagct tgatgaaaat aatgaagaaa
    23101 ttttaaagct actaacaaat aaaattaata tagtggatga aaaaattaaa gaattagaac
    23161 ttataaaaaa agtactaagc gcaaagaaag agaccatgga aaaaggtatg aaagtagatg
    23221 aagttggtaa aatagtgaga aaacatattc cacgaagaag tatcttatct atagagtatg
    23281 aagaaggatt aagtagtgaa gaaactatgg aactttcaag aagacaaata gcaaatatat
    23341 tagaagataa tatatctgta ttctatggtg gcgtaagtgg acttgtatct atagataaat
    23401 ttttaaagga agataaagtt atttatgaca atagttttgt tactgttgaa agagatttat
    23461 ttgatataaa atcacaaaaa tatataacgg aaatttcagc tggtgatttt atttgtatga
    23521 catataaagg accttacaaa gaaaattata ccgcttttaa gaagctagtt gattatgttg
    23581 aatataaaag aatacctatt gaaaattata tatatgaaat acctataata gaccctctgt
    23641 ctacaaacaa tgaagaagaa cttctaacag aaattcaaat acaagttaaa aagagagaaa
    23701 ttgacttaag atgtctagtg tagtaaccta gtttacttac aaatcttaat ataattgtct
    23761 attatcaaca tacgattttt ttagataatg attcctaaca gtgagatttt caaaaaatag
    23821 gcgggagcat ggctaatgtc aattcccacc tatttttaat taagccatgt catcacgaag
    23881 tgcatcaata atattttctt tttttatctt attaatagta tacaacattg taataaatac
    23941 gataaataga acactaaata tacttattcc aatactaacc catggaatca caaaatcgat
    24001 atcatctgta accattactt tatgaattag ccatgaagaa attactgcta taggaagacc
    24061 aaagaacaac gccctcatac cataaaaagc acactcaaaa ttcatcatct tttggaaatc
    24121 atgctcagac attcctacag agcgaagcat ggcaagttct ctacgacgta acttaatatt
    24181 agttgaaatt gtattaaaca catttgcaat cgaaatcaga gaaatcataa ttatgaaagt
    24241 atacgcaaaa acattggcaa taaaaatgta attacggttc tcatccatca ttccatacat
    24301 attgtacaga ttgtactcat tcgtaactcc ctcactctca ataatttgct ccatttctga
    24361 aactgactga gttggatttt ttgattgaaa gcttaaactt tcaattcctg tagaaacacc
    24421 aggaatctca aacttatctt tagctgaata aggagccaat actctgaagc ttgctttctt
    24481 catatctgag tttctgattc ctggaggaga atcaggtggc accactttga caaatgaaat
    24541 atttacattt tgtctctcag gcgtgtccaa cttgccatta acttcaggag tgaggctaac
    24601 atccatagaa gaattggtaa aaatatcaat aatatgatta tctttcgtta acatagaaac
    24661 ggcaatcatt tttgcatttt gcccagtata ttcttctgtt ggcaatccaa gacttttgat
    24721 aatattcaga taagttctat catcaagaaa ctgcatatct actggcaatt caagcatttc
    24781 actcatagaa tgtaaaccat tatagttcaa ataatcgtca gtaagctcac ttactttggc
    24841 aacacatgaa taggtcatca gtttctgata caaaccttcg taaacaccac ttgcagtttt
    24901 tagcttatca taaagcttca acatttcgct gtctttcatg ccttgtgtag aaaaaataat
    24961 gtcttggtca gtaacctctg acctctgatt tgacatctgt tttaggtctt ttactaaagc
    25021 acttgtagag atgaatagaa ctatgcttaa aacaagcgac aatacaatac ttcgataacg
    25081 tttcttgttt cttttaaagt ttttcatagc aagaactccc tcaagaccat aaatttgctg
    25141 tgttagcttt gatgtcttca cagatttaga ttcaattttt acctcatttg tctgacgaat
    25201 acactccatc actggtgtgt tggcagcctt tcttgctggt atgtaagcag aaattaagat
    25261 tgtaaccatg cttaccactg ctgcaacgac gattgcaggt atagataatg tcagtgttaa
    25321 tggaacattg acgtagagaa tatttgcaaa gtttttagct attacagaaa tcacaagtcc
    25381 aatactgact ataccgatta caacaccaat tggtatacca atcacaccaa tacaaattcc
    25441 ctcaaataac actgaatttc gtaactgttt tgctgtagct ccaacagatg aaagtattcc
    25501 aaattgatgt gttcgctcgt tcaatgacat attaaatgaa ttatatatta gaaaaattga
    25561 gcctagcata attatggcaa gcacaacgcc accaaccgag tataaaaaca tattgaacaa
    25621 attattatca gaaataccca tgaaacgcaa cacattatca ttgaaaatat aatcatgctc
    25681 tctagcacta cttgtataag agcgaacatg acgtggattt ttcaatgtga caaaaaggct
    25741 aagactgtca gcctttactt gttcatcttc tttggttatc aaggtatatc ctggtgcaga
    25801 agattcttca aatacaggct ttctacagat acccacaact gtataagttc tcttattttg
    25861 tggaataaaa gcttcactcc cagcagtgta tgggtcactc tgaccaagat tttcattttc
    25921 actcattcgt ttccctacat ccaaagaaat agtatcacct actgcaaatt tgacaccacc
    25981 atctgtcgca acttttcctg aaacaagaat ctctctacta ttttcaggta atcttccaga
    26041 aattaaagtt ataggcaaag tatcaaaagc ttctttgctg aatccagcaa taaaaagata
    26101 tggtttatct ggatttctgc taccctcaag tgttgcatat ccaatatttt caaatgttac
    26161 agtattttca acttcatcat tgaccctctg ttcctgtaca aaagaagaat caacatccaa
    26221 aaactcgaca tgccaatcac catattttat aatggcacca tttatcatat agttcatcaa
    26281 agaaactcca aaggtagtga ctgctgtaat catagcagct gacaaaacaa ctcctataat
    26341 tgttacaaaa gttcgagtgc gattcttttt caacccttgc agagcaactt tgttaaaaat
    26401 attcattaac ggttcacctt cctctcatca cgcactactt ttccatctga aatacagata
    26461 attctatccg cctgtaaagc aatgttttca tcatgtgtga caatgatgag agtttggtga
    26521 tatttactat ggctttcttt aagtaactgg ataatttctt gtccattttt gctgtctaaa
    26581 ctacctgtag gctcatcagc aagcataact gctggtgcat tcatcaatgc acgaccaata
    26641 gcaacacgtt gctgttgacc cccagaaagc tgatttggta aatgtgtttt tcgttctttt
    26701 aaaccaagta actctagcaa atcatttaac ctttcttggt taaccttacg cttatccata
    26761 agtataggta aggtgatatt ttccactaca tttagtgttg gtataagatt gtgaaactga
    26821 taaatcaatc caacttgtcg cctacgaaaa atagcaagtt tttcattgct ctgaccatat
    26881 acatcttgac cctccagata tatctttcca cttgttggta catctactcc tgcaatagaa
    26941 tgtagtagtg tagatttacc agaaccagaa gaaccaataa ttgcagtaaa ctctcctttt
    27001 tcaatagtaa gtgagacatg gtcaagtgca gtaacctgat tttcattctt accataaacc
    27061 ttacataagt tttcaatctt taaaaatgcc attatataag tctcctttca tatttcataa
    27121 atatatcata gaactttcaa cttacattta agtgacattt aaatgagaga ttcgtcactt
    27181 tggaaaacga ataacaaata ttgcaccacc ttgtgggtga ttttctgcag taattgttcc
    27241 tccttgcctc ataataatcg tcttacaaag tgccaatcct attccataac cagctgtgct
    27301 tgaatttttc ccacgataaa atctgttaaa cagacatggt aaatcttctt ttttaaagcc
    27361 ttctccattg tcatgaatag taagttgagt aaacaaaaag ttgtcctcac aaacaatatc
    27421 aattttccca ttatccccca cactctccat gcagttttta aatatatttt gaattgcttc
    27481 tgaaagccaa tttaaatcgc ccaaaatctt tactcctttt ggtatattaa tatgtagagt
    27541 gatattgtgt agttccatag gaatcaaaag ttgatgaagt gcagaactaa tcaaattatc
    27601 cacatcaatt tcttcttttt gaaaaactac aatacctgca tctaaacgtg ataattttaa
    27661 aagtgaagta atcagccagt ccatttgtat aagcaattct tctgtttctc gtatcaactc
    27721 ttttctctca tcttcatcag aagtattctc taacagtgac aaaataagag ttactgatgt
    27781 aagaggagtg cgaagctggt gggctatatc ggctaatgaa tcagcaagat gttctttttc
    27841 ttttttcagt gcataatttt gttctctaat acgtaacgtc attttagtta tttcactttg
    27901 caaaatagaa agctcaccct catctgattc agtaatatac agatggttag cattatgaag
    27961 cacaagatta atttgttctg aaatctgtgc aatacttttg tatcgatttt tagtaaacac
    28021 aaaaaatgct gttccaaagg tagtggcaga aacaatagaa attattccag tcatagtact
    28081 gattgcaaat cccagggcta cagtgagaat agtaattaat aaaaataaaa tagcaaatct
    28141 tcgaaactct ctatttcgaa acataacttc ctcccaatct ataccctgtc ccacgaacag
    28201 tcaaaattat ttgcggactt gaagggtcat cttctatctt ctctcgcaag cgtttgatgt
    28261 agacagtcaa tgtattgtca ttaacaaact cacctgctgc atcccacaat tcatcaagta
    28321 atctcactct tgtaataata cttttaggat tgctaataaa caccaataac aaacggtatt
    28381 ctaaagctga aagaaaaacc tcattgccat tctttttcac aacaccggtt gccgtatcta
    28441 cataaagccc acaaatttca aaagttgacc caaaacgccc actttttcgt agtgctgttc
    28501 taattcgcgc aatcagttca cgtggacgaa aaggtttagt gatatagtca tctgcaccca
    28561 tattcagccc agtaacaaca cttgattcat caccagaagc tgtaagaaag ataacaggaa
    28621 tatcttgatt ttctttgatt tctgtacaaa tcgtaaaccc atttccatca ggtaaagaaa
    28681 tatcaataag tgccaaatca aatttattct tagcaatctc tacaagtgct tcacttcttg
    28741 tggatacatg ggttactata aatccttctg aattaagcaa aagaataaga tttttggcaa
    28801 ttgctttatc atcctcaact aaaaatatcc gtttcattta ctttcatcct cctttatttt
    28861 attagtttta tatattccag cagagtaata atacactact attttactta ttattacaca
    28921 attttcatta gtttcttttg ttcttataca atctatgcat aatacctcca ttgatttatt
    28981 atatttctta taacagaaat acacaagata ggataaaaaa cataatgtaa gtgcaactat
    29041 tttttaattc actaattcac taacttataa acacacattc caatgcacgc cacctccctg
    29101 atgatgtact tatcaatcta catgtttact atggtactcc catgttagta tacactttat
    29161 aatcaatact tgaaaaacta agctcaatgt tttattatat acttatatat accttatatt
    29221 ataaggtata tgataaaata aagtaggtga atctatggac ttgaataaag aagtacttaa
    29281 aggtcatatt gatactttaa tattatcaat actaaataaa ggggattcat atggttatga
    29341 aatagcaaaa atagttataa atgaaacaac ctttgaatta aaggaaagta ctctctatgt
    29401 atctcttaaa agactagaat ctaagggtct aataaaatca tattggggaa gtgaccaagg
    29461 acttggaagt agaagaaagt attacaatat aacagatgaa ggaaaagaaa atttagaatt
    29521 aaagattcaa gagtggaact ttatacaaag cgttatgaac aaatttctaa agagagggga
    29581 atagatttat gaaaataata gaagagtatt tagaagcact atataaaaat gatactagca
    29641 aagaaattca agatttaaaa gaagaattaa aagagcatct aatagattcc actaatgaat
    29701 ttattgagaa tggatatgac ataaatgaag cacaaaataa ggcaatagaa agatttgatg
    29761 gcagtaatgg agtatcagta gaattacgtt caatctttaa acaaaaaata gaacctaaaa
    29821 aagtactatt acgaaaatta tcaaattcaa gaattgtatt tttaaattta tttggatggc
    29881 ttcttgcagc tactgcatat acaataaata gaggttttgg tcatatagct ccaccatgga
    29941 taattatact tttaataata tcaatagtta tattaataat cttaactatt ataattaagg
    30001 gattaaaaaa ggaaatagaa aatgattaca atatatagtt atacagttat attgtaatat
    30061 attatttatt cttataaaca catattttga aaggagcttt ttatatggtc aaaaaaattc
    30121 tattagaaaa atttcttgat acctcaggag tttcatatat caatgaaaaa ttgaatctat
    30181 ttaaggatac taaatggatt tcatttggca cctatataat gaccgttgat agtttttgtt
    30241 atagagagga ttcaaagcac tataatccaa taatgtctac ctttgattat gaatcagaga
    30301 tttttatagt taatgcctta tacaaggaaa atatctattc tattaaaagt aaaaaactct
    30361 ataggaatgt atctaaagga gataagataa ttgttacaat taaatactct tatgataaat
    30421 atgaaaatct tatacatgaa gaaattcatg actatcatct ctatcctctt tctcttgatt
    30481 aaaataaaag gctatagcct ctatagctaa cgcctttaat ttaaactact ttgaatacta
    30541 tataattttc ataaaagact gtaaataaat ttgtgcaaaa tatataaaat ttatctacag
    30601 tcttttgtta tacaaaattt ataatctctt atagaacaaa tttgccattt ctcaacttta
    30661 tcacttttac tcagctatat tataaacttt taatttatct ttatttaaat gtttcttctt
    30721 aacttctact aaggctttta gtgtatccaa agatgtcatt atttcagtag aaaattcaat
    30781 agctgttctt ctcattttaa atccatctct tgtagaatca tttcctttag ttggtgtatt
    30841 tattactata tcaacttgtt tatttcttat agcatctaat atatttggtc taggctcttc
    30901 aattctattt actatatcag cctctattcc attttctctt aagctcttag ctgttccttc
    30961 tgtagcaaca aaagtatatc ctaattcttt catatctttt gctatttcta taaactcatc
    31021 tttgtcatga ttgtttacag tagcaagtac tac
//
LOCUS       DAMJVM010000040        28578 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.041, whole genome shotgun sequence.
ACCESSION   DAMJVM010000040 DAMJVM010000000
VERSION     DAMJVM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28578
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.041"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            192..413
                     /locus_tag="PC523_001924"
     CDS             192..413
                     /locus_tag="PC523_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HDF2475449.1"
                     /translation="MGYLKEDRQDYCCEFARPYVNRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
     gene            413..676
                     /locus_tag="PC523_001925"
     CDS             413..676
                     /locus_tag="PC523_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HDF2475450.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESRYGALTNFGYESSEHPWSWISEPWPWDKSFYK"
     gene            740..1312
                     /locus_tag="PC523_001926"
     CDS             740..1312
                     /locus_tag="PC523_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2475451.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGSCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            2104..2757
                     /locus_tag="PC523_001927"
     CDS             2104..2757
                     /locus_tag="PC523_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HDF2475452.1"
                     /translation="MNFIYSNAEIEYLKHKDKILSDIIDKIGHIEREIDTDLFSSVIH
                     HIIGQQISTKAQATIWKRMKEHLKEINASSILSAGTTGLQSFGMTYRKAEYLTDFATK
                     IQNGEFDLQSITYMSDKEAIDKLTSLKGIGVWTAEMILLFCLQRPNIFSFDDLAIQRG
                     LRMVYHHRKITRKMFEKYQKRFSPYCSVASLYLWAVAGGVIDGMRDYAPKGKVRKNG"
     gene            2919..3452
                     /locus_tag="PC523_001928"
     CDS             2919..3452
                     /locus_tag="PC523_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDF2475453.1"
                     /translation="MQYINYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGEEPNFTPSLHMVGTPFQTAVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIVPCHRVVGTNRSLTGYAGGIDRKIKLLSL
                     EKVSMQHYFIPKKGSAL"
     gene            complement(4006..4791)
                     /locus_tag="PC523_001929"
     CDS             complement(4006..4791)
                     /locus_tag="PC523_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HDF2475454.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTISDMQGNIGDMQGSI
                     SGIEGTISGMQGTISDMQGNIGDMQGSISGIEGTISDMQGNIGDMQGSISGIEGTISS
                     VQGTIIDMQGTISDMQGNIGDMQGSISGIEGTISGMQGNIGDMQGSISGIEVTISGMQ
                     GNIGDMQGEIKGVNSRLDNMEPNIKDLKTGQVETSSKLNTIQSDVTEIKSDVKINRNT
                     LDTIIKHTSSLVEDTSDIKNDLNNIEIVTSKNWNDIAKLKSVK"
     gene            5255..5668
                     /locus_tag="PC523_001930"
     CDS             5255..5668
                     /locus_tag="PC523_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2475455.1"
                     /translation="MKMPKENVDMIMFAPCGMNCIVCYKHCYHKKPCAGCLNSNMGKP
                     EHCRKCKIKDCVCQKGLFYCFECFDFPCKLIKNLEKSYNKRYQVSLLENSKFVQKYGL
                     EKFMEKQRERYTCSKCGGIISIQDRECSECREKMK"
     gene            5742..6278
                     /locus_tag="PC523_001931"
     CDS             5742..6278
                     /locus_tag="PC523_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDF2475456.1"
                     /translation="MIGLKCGMVKLLKHQIIWDKSAKDVIILLKSILDKTAIDIQHIG
                     SSSIPSVSAKPIIDIVVGVASLEEAKLYIKKLEQRKIIFRGQDIPEQLLFVMGDLKKN
                     IRTHHIHVVEWNSFAWNNYINFRDYLNTFTEKAKLYDSNKQKLALQFEDNRKIYTQRK
                     QELISTLLEEARLWRMNT"
     gene            6439..8013
                     /locus_tag="PC523_001932"
     CDS             6439..8013
                     /locus_tag="PC523_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HDF2475457.1"
                     /translation="MKKDTSHIIKRAEHFQVILIGEGLMVGAVAGLIVLLYRILLGQA
                     GTWLSQILKFVEGSTIKIIAWFAVLAVLAWIVSKLVDWEPMISGSGIPQLEGEMTGKI
                     KQVWWRVLPTKFIGGFLSLMGGLALGREGPSIQLGAMAGKGISRALDRGKTEEKFLLT
                     CGASAGLSAAFHAPLAGVMFSLEEVHKNFSVSVLISVMTASLTADYISANFLGMESVF
                     QFDIGNVLPQDYYGLIVILGVILGVMGAFYNWFTLYVQYLYKKIPKIGTFGKLLIPFI
                     MAGILGLVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGSGAPGGIFF
                     PLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTGIILIFEMT
                     GSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGEKILDQFSV
                     MFGSPVANKAIQDIQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMTDERDSGAV
                     YDKMENLCKEHTSHIF"
     gene            complement(8702..9307)
                     /locus_tag="PC523_001933"
     CDS             complement(8702..9307)
                     /locus_tag="PC523_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HDF2475458.1"
                     /translation="MINWNLWRTLNMEYIINPCSGIKIKVTKGQKIEIVDIEGGQVVD
                     FFAEMQDDSAEFISPGVTIDCNESLRLQVGNLMYSNKYKPMFKILYDDVREHDLLHPC
                     CKLEMYDFFYNNGENHPNCFDNINQALGTSHQIIHPINLFMYTKLNTDGSIEVCEPLS
                     KANDKIVLEALENMTLGIAACSVSESSCNSRKCTSIKIVVD"
     gene            9678..10247
                     /locus_tag="PC523_001934"
     CDS             9678..10247
                     /locus_tag="PC523_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDF2475459.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTEILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESSGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPSVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKMLYSNRYN"
     gene            complement(10584..11858)
                     /locus_tag="PC523_001935"
     CDS             complement(10584..11858)
                     /locus_tag="PC523_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2475460.1"
                     /translation="MTFLLYKLTSYFIDFRNHWIFKIILIYGYSIISQMIIWISDPVN
                     ILFTLFGYFVLLIICSKSRLIPIVSVIMILYPIIVGINFLFINNPLYRELIISKEYIL
                     ILSIVQIVSALIKLLFWFLIHIFLKNRLVNIKQYLSNKIWIFIDMVCISSFLSILIAI
                     ILPPTNLIPMGSNKNIILGNLSYGTYLIILSAILANIGVILLLQPLIENVKMKIEKQA
                     NHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLEIEDIAGAKKYFNQLKIGIGTL
                     SGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDNHLGIEPIDLCTIFSNSLDNAI
                     EASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPINRKQELFISDKKDSNSHGYGI
                     ENIREIVNKYNGKLDISYTENEFSLFLYIRLF"
     gene            complement(12152..12328)
                     /locus_tag="PC523_001936"
     CDS             complement(12152..12328)
                     /locus_tag="PC523_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475461.1"
                     /translation="MFFTFIGVISAILLLCTIIYGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            complement(12432..13040)
                     /locus_tag="PC523_001937"
     CDS             complement(12432..13040)
                     /locus_tag="PC523_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2475462.1"
                     /translation="MEKFLRGKIMKADSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINTSIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENHNNKKTEAEYRTIAYQITSF
                     IQLIFLRSNAFLKYSGIDILNVEQRNGLIDFQLDLFLVDITK"
     gene            complement(13136..13960)
                     /locus_tag="PC523_001938"
     CDS             complement(13136..13960)
                     /locus_tag="PC523_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2475463.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISTLQRIVLLRDSKFSIAEIANIIHDWNDEFVIKELNRKKNEIQKEIKQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISIREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQPNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHRGEHNEINCENYLTEIQIPLIQV"
     gene            complement(14126..15019)
                     /locus_tag="PC523_001939"
     CDS             complement(14126..15019)
                     /locus_tag="PC523_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2475464.1"
                     /translation="MQLYNTPFNTNKNLIYFFTLTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEMGIKWPLLTILFFALTFIT
                     SLTIGNYFNVKTSDIDWRQIVILRPSQIAYMLLVSFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFSVQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDL"
     gene            complement(15273..15710)
                     /locus_tag="PC523_001940"
     CDS             complement(15273..15710)
                     /locus_tag="PC523_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HDF2475465.1"
                     /translation="MKIHVEQGINCDDIEITIKCSTLDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLRNNEKVIINRCYIKNIKEKLNI"
     gene            complement(15967..16116)
                     /locus_tag="PC523_001941"
     CDS             complement(15967..16116)
                     /locus_tag="PC523_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HDF2475466.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LLFKK"
     gene            complement(16121..16360)
                     /locus_tag="PC523_001942"
     CDS             complement(16121..16360)
                     /locus_tag="PC523_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HDF2475467.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIRIGG"
     gene            complement(16685..16906)
                     /locus_tag="PC523_001943"
     CDS             complement(16685..16906)
                     /locus_tag="PC523_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HDF2475468.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVIKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            17485..17742
                     /locus_tag="PC523_001944"
     CDS             17485..17742
                     /locus_tag="PC523_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2475469.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVTKLYGGTNTYIPTYNGL
                     FRYARNREIVKQFNGVNYNELAIRYNMCVSNIKRILNENSV"
     gene            18190..19428
                     /locus_tag="PC523_001945"
     CDS             18190..19428
                     /locus_tag="PC523_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDF2475470.1"
                     /translation="MNNGFYKLNSAVLNFDIKSDVNKIRFNHYIMRKENIQRLQGTLP
                     RGQFYMTVRRAATDLQLSISTISRLVREFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKHMELDCVCNDEFVDNSDIELDCNSKDNSFNGDINVETEYCSNEYLFNNDVE
                     HNYDNFYNKDTIKNKSIFNISTIKKYINESFDTNKSENSNLQQESINFNFNDTCNAID
                     KRTNNSIKSNQNKKAMKLSDINVLSDKDITNKKNYFNHTHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNIHPCKEGNSNDSSNYIHSNYTTYDD
                     SVRERKKYENVRYWNPICEI"
     gene            19897..21198
                     /locus_tag="PC523_001946"
     CDS             19897..21198
                     /locus_tag="PC523_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2475471.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDKKSLAIKTSDVTNIITWGQNFGLDGHIKILKENLA
                     RRSINQTCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKVD
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            21274..21549
                     /locus_tag="PC523_001947"
     CDS             21274..21549
                     /locus_tag="PC523_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2475472.1"
                     /translation="MLELTKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELIIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            22607..22837
                     /locus_tag="PC523_001948"
     CDS             22607..22837
                     /locus_tag="PC523_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2475473.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            22830..23411
                     /locus_tag="PC523_001949"
     CDS             22830..23411
                     /locus_tag="PC523_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2475474.1"
                     /translation="MNNKKHWYDYLWIFSSVYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMMLFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(23985..24662)
                     /locus_tag="PC523_001950"
     CDS             complement(23985..24662)
                     /locus_tag="PC523_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2475475.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTIRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            25193..25714
                     /locus_tag="PC523_001951"
     CDS             25193..25714
                     /locus_tag="PC523_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2475476.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKEVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPETAKAVLEQQLVLAQSL
                     ETWINSLKDNMGN"
     gene            26097..26894
                     /locus_tag="PC523_001952"
     CDS             26097..26894
                     /locus_tag="PC523_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2475477.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKRVVLIGQSAGGF
                     VAQSFIVKYPNMVEGIMMIGTCPYGIDYYSKLDIFWLKQTEWMLKLFPDRLLRNIISK
                     MCGATSEGRRSMLKMLEGYEKKELCHLMYLGFAGLIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQL"
     gene            27274..28164
                     /locus_tag="PC523_001953"
     CDS             27274..28164
                     /locus_tag="PC523_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2475478.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKKAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFEAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTVQNKDGCEKLLREECARYGILTD
                     MKEIIKAYKRSESYTQIKFF"
BASE COUNT        10652 a         3288 c         4639 g         9999 t
ORIGIN      
        1 ctatattact catttaaaat agatatttat gaaagattaa ttagaaaatt ttagaggtaa
       61 aataagaaca aaaaaatact tatttaatct tattaaagac ctattaaaac tactgattat
      121 aatatagaac aagtatacaa tttttaagaa taatgctatt ttaaaattct aaaaattaag
      181 gaggataatt aatgggatat ttaaaagagg atagacaaga ttattgttgt gagtttgcta
      241 gaccatatgt caatcgtcaa atttatgatg acacatacaa tccaaaaaca gcattaaaaa
      301 gaggtacttt atttcctgaa ttagatttaa tagagtcaaa taactataat gattggctat
      361 atgcaaatcc taaaaataga gttagagcta aaaaaggggg taacatgtac taatgtcatt
      421 aaatagaaga gaacttttag agagaatttc agaatatcaa tttgcttgta tagaattaaa
      481 tttatattta gataacaacc caagggataa gaaagctctt gattcatata acagatactg
      541 cgataaattt acacaagctg tgtgtgatta tgaatctaga tatggagcat taactaattt
      601 tggatatgaa tcaagtgaac atccttggtc atggatatct gaaccttggc catgggataa
      661 atcattttat aaataaaatt attaagattt aaaattagaa tttattatta aataatatat
      721 taaggaggag taagataata tgtggattta tcaaaaaaca ctggaacatc cagttaacat
      781 aagacaagca gaccctagaa tggcaaaata tatcatgact cagttgggag ggcctaatgg
      841 tgagctggca gctgcaacta gatatcttca acaaagatat actatgccaa ctggaaaatc
      901 tcgcgcactt ttaactgata taggtacaga ggaaatggct catgttgaga taatttcttc
      961 agtgttatat caattaatag gcagttgtac tccagaagaa cttaaggctg ctggacttgg
     1021 cagtaattat gctaattttg gacatggtct tcagccagta gattctaatg gagtaaattt
     1081 tactacaagt tatattaatg tctttggcga ttcggtaact gatttacatg aggatatggc
     1141 tgctgaacaa aaagcattgg ctacgtacta tcaattaata aatttaactg atgaccccga
     1201 tttaaaagat atattgagat ttttgggtga aagagaagtc gttcattatc aaagatttgg
     1261 tgaagcacta atggatgttt acgagtttac agagtgtaag catcaattct aatgaactat
     1321 taaaagttga cagagagtaa gccttagttt taatgaacta ttaaaagtga accagagagt
     1381 aagcattaat tctaatgaac tattaaaaat aagaggtaaa ggttttagtt gatttccttt
     1441 atctcttatt ttttactgat taaacagatt actttgctat ttaaacatat tattttatca
     1501 aagaaaagct caatcaaaaa ttcaaagcat caccatagtt caaggtatta ccgttgttgg
     1561 caagttttct acaatatgtt ttctttaaaa catttactaa aataacttat ttgatgaaaa
     1621 acaattttta tagcagtatt acttattagc tcatttgtac attttagaaa ttgagtttct
     1681 tttaataagc gaaactgagt aaaatactta tggggtgtaa tatttagact tagtttaaag
     1741 catatggagt actatgattt acttatatta acactgtttg ataagcattt caaagttaag
     1801 ttttcacaat agtgtttcta aatataacgt aaaatttttt gcatacagat attaactgta
     1861 ttttgtattt tattagtggc aaaacaatat tttttatcat aataagccaa atggaacaaa
     1921 tagaaactga tgtttcatac atataaagtt cattttttct ttttcacagc ctattcttac
     1981 ttcctacatt tgagagtatt ataatattat ttgatgttat atcgatattt ttttgaccta
     2041 tttagtgata aaattataat atgagcttat ctaaaagtta atatagtagg agggctatga
     2101 tatatgaatt ttatatatag taatgctgaa atagagtatt taaaacataa ggataaaatc
     2161 ttgagtgata taatagataa aatcggtcat atagagcgtg aaatagatac agacttattt
     2221 tcgtcagtaa tacatcacat tattggacaa caaatatcaa caaaggcaca ggctactata
     2281 tggaaaagaa tgaaagaaca tttaaaagag ataaatgcaa gctccatctt atctgctggt
     2341 actacaggat tgcagtcttt tggtatgaca tatcgtaaag ccgaatattt aacagatttt
     2401 gctactaaaa tccaaaatgg tgaatttgat ttacaaagta tcacttacat gtctgataaa
     2461 gaagcaattg ataaattgac ctcattaaaa ggtattgggg tttggacagc agaaatgatt
     2521 ttattgttct gcttacagcg tccaaatatt ttcagttttg atgatttagc aattcaaaga
     2581 ggattacgaa tggtttatca tcacagaaaa attactagaa agatgtttga aaagtaccaa
     2641 aaaagattta gcccttattg tagtgttgca agtttgtatc tttgggcagt tgcaggtggg
     2701 gtgatagatg gaatgagaga ttacgctccc aaaggtaaag tgagaaaaaa tggttgaata
     2761 tatgcaggta aagataaaac tagtatgata tgaggataga tattataacg aaagtgcaat
     2821 caggaggaaa ttatactagt atgtttttac ataatggcag gtagaaaaga agtgtctaat
     2881 gactcgaatg aaattttcag aatataagga ggcattgtat gcaatatatt aactattacc
     2941 atagtccaat tggaaatatt cttttagcat cagataatat tggattaact ggtctttggt
     3001 ttgagaatca aaagtattat gcttataatc ttgaccaaga acatcaggaa aaagaattgc
     3061 ctattttcga gcagacaaag aaatggctag atatttattt ttcaggagaa gaacccaatt
     3121 ttacaccatc actccatatg gtaggtactc cttttcagac tgcagtgtgg aagattttac
     3181 aacaaatacc atatggtaaa acaatcactt atggagaaat cgcatataaa gttgctcaac
     3241 aaaaaggaat atctaaaatg tcagcacagg caattggtgg tgcagtaggg cataatggaa
     3301 tctctattat tgttccatgt catcgtgttg taggaacaaa caggagtttg acaggatatg
     3361 ctggtggaat cgatagaaaa ataaaattgt tatctttaga aaaagtgtct atgcaacatt
     3421 attttatccc aaagaaaggg agtgcattat aaagtcatca aatttagaaa ttcttaatca
     3481 ttcctttgat atacaagtat ctgattttga aagcaataac ttgcaattta aaaaaagatt
     3541 ttttagacta tatgttatac aatattatcc atagtaaaca ttatacaatt ttagagatag
     3601 gttttggagt ttttatttta tcattgtaca aatgttcaaa atatgttttt ggaaatgata
     3661 agagtgttaa atccaaagga aaaaatgaaa acaagatttt atccttttat aagtgataag
     3721 ggtatgtgtt ttaatcaaag atggactttt actttaatgc taaaaattat atagccatat
     3781 tataatatat aagggagagg cacacctttt tagtgttcaa tctcccttgt tttttattat
     3841 tatatcaaga ctcattcagt cgtgttaatt ttatgataaa atgagtattt tttaataaat
     3901 tatttataag taaagtgtgt tatcggataa tcagtatttt tgtataaaat ttggtataaa
     3961 tttaaagaag tatgtgaaat tttcaggtgt ttttactata gtatattatt ttactgattt
     4021 taatttagca atatcattcc aattcttaga tgtaactatc tctatattgt ttaaatcatt
     4081 tttaatatct gatgtatctt ctactaatga agaggtatgt ttaattattg tatctaatgt
     4141 atttctgtta atttttacat ctgacttaat ttctgtaaca tcactctgaa ttgtattaag
     4201 tttactacta gtttcaactt gtccagtttt taagtcttta atattgggtt ccatattgtc
     4261 taatctacta ttgactcctt taatttctcc ttgcatatca ccaatattac cttgcatgcc
     4321 actaatagtg acttctatac cactgatact gccttgcata tcgccaatat taccttgcat
     4381 gccgctaata gtgccttcta taccactgat actgccttgc atatcgccaa tattaccttg
     4441 catatcgctg atagtacctt gcatatcgat gatagtacct tgcacgctgc taatagtgcc
     4501 ctctatacca ctgatactgc cttgcatatc gccaatatta ccttgcatat cgctaatagt
     4561 gccctctata ccactgatac tgccttgcat atcgccaata ttaccttgca tatcgctgat
     4621 agtaccttgc atgccgctaa tagtgccttc tataccactg atactgcctt gcatatcgcc
     4681 aatattacct tgcatatcgc tgatagtgcc ttctatacca ctgatactgc cttgcatatt
     4741 actaatatta ccttgcattt cctgcaataa ctctaatatc ttttcatcca tcactatcac
     4801 actcctattt tattattata acataattta ggagttgtat tccacaaacg ctatatcata
     4861 tttaattttt aatcatatca ttatctaata ctcaaaacat ggttaaatat gtttatattg
     4921 aaaacttttt tattaatgaa ccttatattg tctaagtgtc taatatacta tgataagtgc
     4981 ttttgaggta tgtgtggtta catctgtatt taaagttgct ggtactatca tatactttta
     5041 aataccactt aagctattta tattgattac ttgtgtttga atattttgat tagtagattt
     5101 acttttatct ttttttattc ttagttttaa actttcatat ttccttgaaa atttattgac
     5161 aattaaagtg catgaatttt ttagttactt tttatgttac aatatgaata tataacaatt
     5221 cgacaaattg gatttttgaa aggagtaaaa tgttatgaaa atgccaaaag agaatgttga
     5281 tatgattatg tttgctcctt gtggaatgaa ttgtattgtt tgttataagc attgttatca
     5341 caaaaagcca tgtgctggtt gtttgaacag caatatggga aagccagagc actgccgtaa
     5401 gtgtaaaata aaggattgtg tttgtcaaaa aggattgttt tattgttttg aatgttttga
     5461 ttttccttgt aaactcataa aaaatcttga aaaaagttat aacaagagat atcaggtaag
     5521 ccttttggaa aatagtaaat ttgttcagaa gtatggttta gagaagttta tggaaaagca
     5581 gagggagaga tacacttgtt ctaaatgtgg aggaattata tctattcaag atagggaatg
     5641 tagcgagtgt cgagaaaaaa tgaaatagct aaatacctgt ttatcaaaat agaaaaaatt
     5701 gtagtatgta tataaaggtt gattatgttg aggtggttat agtgataggt ttaaaatgtg
     5761 gtatggttaa attattgaaa catcagataa tatgggataa gagtgcaaaa gatgtaatta
     5821 tattgttgaa aagtatattg gataagactg ctattgatat tcagcatatt ggaagtagct
     5881 ctattcccag tgttagtgca aagcctatta ttgatattgt tgtaggtgtt gcttctttgg
     5941 aagaagctaa attatacatc aaaaagttag agcaacgtaa aattattttt cgaggtcagg
     6001 atataccaga acaattactt tttgttatgg gagatttaaa aaaaaatatt cgtactcacc
     6061 atattcatgt tgttgaatgg aatagttttg catggaataa ttatatcaat tttcgagatt
     6121 atctaaacac ttttacagaa aaagcaaaac tctatgatag taataaacaa aaattagcat
     6181 tacaatttga ggataatcga aaaatctata cacagagaaa acaagaacta ataagtactt
     6241 tgctggaaga agcacgactg tggagaatga atacttaaaa agaataaatt ccaatttact
     6301 gagaaattta acgataattt tcttatactt agtaaataaa aatctatctt tggtgattaa
     6361 aaaatagctg taaacactcg tgactttggt catgggaaat ttagattgtt tagaattatt
     6421 ggatgaagga ggaaacgtat gaaaaaggat acatctcata ttataaaacg ggcagagcac
     6481 tttcaggtaa ttttgattgg agaaggactt atggttggtg ctgtggctgg tttgattgtg
     6541 cttttataca ggatactttt agggcaggct ggtacttggc ttagccaaat tttaaaattt
     6601 gtagaaggtt ctacaataaa aataatagct tggtttgcag tgcttgcagt acttgcatgg
     6661 attgtatcaa agttggtgga ctgggaacca atgatttctg gaagtggtat acctcagctt
     6721 gagggtgaaa tgacagggaa aataaaacaa gtatggtggc gagtgttgcc aacaaaattt
     6781 attgggggat tcttgtcact tatgggtggt cttgctttgg gaagggaagg tccttccatt
     6841 cagcttggtg ctatggcagg taaaggtatt tctagagcac ttgaccgagg aaagaccgag
     6901 gaaaaattcc tgttaacctg tggagcaagt gctggattat cagcagcctt tcatgcacca
     6961 ctggcaggtg ttatgttttc acttgaagaa gtccataaaa acttctcagt atctgtttta
     7021 atttctgtaa tgacagcatc tttgacagca gactatattt cagccaattt cctaggaatg
     7081 gagtctgtat tccaatttga tattggaaat gtattgccac aggattatta tggattaata
     7141 gtgattttag gtgttattct tggagttatg ggagcatttt ataattggtt tactctatat
     7201 gtgcagtatt tatataagaa gattcctaaa attggtacgt ttggaaaact actgattcca
     7261 tttataatgg ctggtattct tgggcttgtt atgcctgatg tgctgggtag tggacacaat
     7321 ttagttgaag aacttacaag tcatgaatat atttttggaa tggttgtttt gatttttgtc
     7381 atacgtttta tattttcagc tgtaagtttt gggtcaggtg caccaggggg aatctttttt
     7441 ccactacttg ttttaggtgc atttataggt ggaatatttg gtatggtagg tgttaaagtt
     7501 tttggaatga atccagatta tgtaaataac tttgtgcttt tggctatggc aggatacttt
     7561 acagctattg tgcgtgctcc tctgactggt atcatattaa tttttgaaat gacaggttct
     7621 gttagccaaa tgctttcact atcagtaatt tcaattgtgg catatattgt ggctacactt
     7681 atgaagtcta aacctattta tgaaagcctt cttgagaggt tacttgaaaa aaatggagaa
     7741 cctgtacata aagagagggg cgaaaagata ctagaccagt tttctgttat gtttggttct
     7801 cctgttgcga acaaggcaat tcaggatata cagtggcctg aaaattgtct tttggtagca
     7861 attcaaagag gaggagaaga aattattcct agaggtaaaa caattcttag accaagtgat
     7921 gtaattgtga ctatgacaga tgagagagat agtggtgctg tgtatgataa gatggaaaat
     7981 ttatgtaaag aacatacatc acatattttt tagggataat gatgaatgtt taataatgat
     8041 agaatattta atgaagattt gataattaaa aattggtaat ttgaaagaga attaatgagt
     8101 taatataata aaatgaaaat gtgatatttt agatgtaaaa gagtttgaag aaaagagctg
     8161 gatgtatttg ttaatgagat tatgtggatt aatggtatat ttatattgtg ctaaaagtta
     8221 gtctattgta tgagtaatgg gaacaaaggg cttgaaaaaa ataggtagaa gattttttag
     8281 atattcttat tgggatattt taattaatat attttgattg cgatattttg ggaagaatag
     8341 aatttattta taaatgaaaa taattaaaag gggttaaggt tagatgaata tcgataagga
     8401 agtattatta gataattaac ttaacatcta acagatagag tttttatgaa aacagtgtgt
     8461 attgaaataa aaaggtacac gctgtttttt cgttctgtta cgcaatagaa tgctattttt
     8521 tagagtacga gtataaaacc catattccct tgtaattgtg tctgaaaaac atcataaaat
     8581 caagggattt ttacacataa atgtgtaaca taccataatt aattgaggac gcaagtgctt
     8641 attcttttgc gcccttgatg gtattttctg atttttatat ttatttgata aatgaaattt
     8701 gtcaatcaac cacaatctta atagaagtac attttctgct attgcaagaa ctttctgaaa
     8761 cactgcacgc agcaatccct aaagtcatgt tctccaatgc ttcaagaaca attttatcat
     8821 tggcttttga taaaggttca catacttcaa tacttccatc tgtattgagt tttgtataca
     8881 taaataaatt gatggggtga atgatttgat gggaagttcc cagagcttga ttaatattat
     8941 caaaacagtt tggatgattt tctccgttgt tataaaagaa atcgtacatc tcaagtttgc
     9001 aacatggatg caataagtca tgttccctta catcatcata taaaatttta aacataggtt
     9061 tatacttatt agaatacatt aaatttccaa cttgtaatct taatgactca ttacaatcaa
     9121 ttgtaacccc tggagatata aattcagctg aatcatcttg catttctgca aaaaaatcaa
     9181 cgacctgtcc tccttcaata tccacaattt caattttttg ccctttagtt actttaattt
     9241 ttataccact acaaggattg attatatatt ccatatttaa tgtcctccat aaattccaat
     9301 ttatcattct gttctatgtt ctcattttaa cacatactct gtctcttttc tatttgtttc
     9361 ttgacgttta taacatagtc tatcattttt taattttcat aaaggtgttg tagtcttttt
     9421 tcaagattac agcatttttt tgcttaaatt tggcattttc gtaatattac tgtatttaga
     9481 agtaagaaaa aatattctat atttaaatag atataaaagt gtaagaaagg taaaaatctg
     9541 ttataataca ttaaaaacta aatttagaaa aatttatata attagaaaaa tgatttgata
     9601 aaaaataaag ttaaatacta actaagtaaa tttgaagaag aaaaaatatg aaatacaaat
     9661 ttaaaaggag gtaactaatg aaaaagaagt tggaagatat gactttagag gaattatgga
     9721 acttgtttcc tatatttttg actactcaca aagaatgttg gaaaaattgg tttaaagaag
     9781 aacaatatag actaactgaa atattgccaa tgcaaaaaat tgttagaata agtcatattg
     9841 gaagtacttc tgtgagtacg atttatgcca agcctattgt tgacatactg attgagataa
     9901 ttcctgatta taatatgaaa gatattaaag atttgattga gtccagtgga tatatttgta
     9961 tgtctgagag cagtagtagg ctttcactta ataaagggta tacagtagat ggatttgaag
    10021 aacgagtttt tcatttgcat ataaggtatg taggtgataa tgatgagttg ttttttagag
    10081 attacttaat tgaccaccca agtgttgcaa aggagtatga aaaacttaag ttggatcttt
    10141 ggaagaagta tgagcataat cgtgatgctt atacagatag taaactagat tttgttaggg
    10201 agtttactga aaaggcaaag atgttgtatt ctaatagata taattagaaa tagatataat
    10261 tagagattta tcttatatat cgcattgtat tttttaagaa agtaattaat agaaaaaggg
    10321 attgtttcaa aatggagaga taaatccctt tttcattgtt ggaatggtaa aaatatattt
    10381 gatttcatat aaaaaagagt gcctcatcaa ctaaaaagtt tattttgaga cacatacatt
    10441 tttactaagg taaataaaaa tatcacattt gattaaattt taagttaatg aatatatgtg
    10501 caaatattta attataagtt agtacaattt taatcagtat aaaacaatct aatttatatt
    10561 atataagata atctaatttt agactaaaat aatctaatat aaagaaataa agaaaattca
    10621 ttctcagtat aagagatatc caattttcca ttgtacttat taacaatttc tctaatattc
    10681 tcaataccat atccgtgaga attactatct tttttatccg aaataaatag ttcctgtttt
    10741 ctattgatag gatttacctt gttgtttgtc aataaatagc taaaatactc cttctcacaa
    10801 cgtgccttta ataccacttt tctatttgac aaatcaggaa ttttcaaaga tgcttctata
    10861 gcgttatcta aagaatttga aaaaatagta cataaatcta taggttctat acccaagtga
    10921 ttgtctatat ctatattaaa gtgcacatca acaccatttt cttccaaact tgtatgacga
    10981 agatttaaaa ttgagttaag agcttggttt ttgcaaaatt gtttgccact aagagtacct
    11041 atacctattt ttaactgatt aaaatacttt ttagctccag caatatcttc aatttctaaa
    11101 tacgattcaa tcatttgaaa gtgattattc atatcatgtc gcattttgcg tatttgaatt
    11161 tgttgatttt ctagtaagtt atagtatttt tcttttaggt gatttgcttg tttttctatc
    11221 ttcatcttta cattttcaat caatggctgt aaaagaagta taacacctat atttgccaga
    11281 attgctgaca agatgattag ataagtgcca taagataagt tgcctaagat aatattctta
    11341 ttactaccca tagggattaa attagtaggt ggcagaataa tagcaattaa tattgataaa
    11401 aatgaagata tacataccat atcaataaaa atccatattt tatttgataa gtattgcttt
    11461 atattaacca atctattctt taggaaaata tgtattaaaa accaaaatag taatttaata
    11521 agagcagaaa ctatctggac tattgaaagt attaaaatat actcttttga gattataagt
    11581 tccctataca aagggttgtt tataaaaaga aaattaatcc caactataat agggtaaagt
    11641 atcatgataa ctgatactat gggaattaat ctgcttttgc tacatattat aagtagtaca
    11701 aaatatccaa atagagtaaa taaaatattt acagggtcag aaatccatat tatcatttga
    11761 ctaataatag agtaaccata aatcaatatg attttaaaaa tccaatgatt tctaaaatct
    11821 ataaaatagg aagtcaactt ataaagtaaa aaggtcatag agtaaggagt gttgtattgt
    11881 aaaaaatagt gtaaataatg tagagtaaat ggaagaaaag gagttataga gcagtaaggt
    11941 tattaagact ctgcaataca acaaaatact ataataaaat aaataagcct ctgtaatata
    12001 ataaaaatta gtaataaata agattctaca atataataaa aatactataa taaaataata
    12061 agcctctgta atataataaa aatactgtaa taaataagac tttgcaatat aataaaaatc
    12121 tataataata aaacccaaca taataaagac attaatttct tactaaaaaa gccactacaa
    12181 tcaacagtga aaaagcgatt gaaagtattg aaaaagtaaa gtgagaatta gagtcattat
    12241 tacgattatt aagttttatc cataaaccat atataattgt acacaataat aaaattgctg
    12301 atattactcc tataaatgta aaaaacatca aaattcctcc cccttatcta attcaagtat
    12361 ttttagtcta tgagaatatt ttcaagcatt accataaaat aaaaaaatgt acataaaaaa
    12421 tcaatatagt attattttgt aatatctacc aagaataaat ctaattgaaa atctattaaa
    12481 ccatttctct gctcaacatt aagaatatca ataccactat acttaagaaa agcattagaa
    12541 cgaagaaaaa tcaactgaat aaatgaagtt atctgatacg caatagttct gtattcagct
    12601 tcagtttttt tgttattatg attttcatag tgagccttaa ttaaaggtag tatctcaaat
    12661 ggataaacaa tttcttcttg tattaatata tagggcacag aaatctttat aaattgacta
    12721 tgctcaccta taaaatcaaa aaatgcaatt agtatttctc ttaattgctg tttaggtgct
    12781 aagtttttag tatcatgtgt agccttatat tcatccatgt gacgctttaa aaatttttta
    12841 atagaagtat taattaactc ctctttagat ttaaaataat aattaatcat agctaaattg
    12901 acatttgctt tagccacaat ttctctagct gtaatacttg aaacatctct atgtgaagat
    12961 agtaactgga ttgtagcatt aattaattgt tcctttgttg aatctgcttt cataattttt
    13021 ccccttaaaa atttctccat aaaaaaatta aacatgttta acacacgttt agatactatc
    13081 atgaaaattt tgggatgtca atgggttatt agtacttata tacttaagta ctaatttaga
    13141 cttgtataag agggatttga atttctgtaa gataattttc acaatttatt tcattatgtt
    13201 cacccctatg accgatttgt ctatttatac ccgatatttg ataatcacta tttttatcta
    13261 accaataaca gaacgattca tatgcaccag ctatattttc atatggacca tacaccatgg
    13321 tacatgccat catatcaatt tcttctgtct cacgatatat aaatccagat ttgttttttc
    13381 caattttctt tacaaccatt ccaacttcaa tatctacacc attatctttg acttcttcat
    13441 catggtaaaa agctatatta ttgttgggtt gacgtgatac ttctatatgt tcctctttaa
    13501 taaatttaga taacttttcc cagagtatac cttcagattg gtagtctggg attatctctc
    13561 taattgatat gattttataa cttggaattt tcttaaatac aacattgtaa tgaatttcat
    13621 ctttatcaca gtttatagct tctataaatt tatcaatttt atttattctc tgttgttcct
    13681 gctttatttc cttttgtatt tcattctttt ttctattcaa ttccttaatt acaaattcat
    13741 cattccaatc atgtataata tttgctattt cagcaatact aaattttgaa tctctcaata
    13801 atacaattct ttgtagtgtt gaaatctgtt caactgagta taatctatga cctgtatatt
    13861 tatctatttc tgccggtttt aaaataccaa gttcatcata ataacgcaac atacgaatag
    13921 aaatttgtgt tagttttgaa acctctccta ttttaaacat gaaaatatga cctccttttc
    13981 acttataatc agtatatatt ttgtttttat atacttcaag agtttgtgga ggtacaatta
    14041 ttttccagat gtcgcaataa attatattta agtagtaatc gtatcaaatt atagattatc
    14101 ttaaatttta taattcatat taaaattaca agtctaactc aaattcaaaa tcatccgatt
    14161 ttatctgttc aatttcagaa tttacctttt gtttaaattt attagaagaa atcatatata
    14221 caagaaatat tagaccaaat attatttgta catattgagc tattacacac aatttaatag
    14281 ggtagtgtag aagtaattga acgctgaaaa aatagtacat catatgaacc aacccagccg
    14341 ataaaatgct tctactcgta tttatatata caaaattaat tataaagttt aaaagtacaa
    14401 tgtttggtat aaataaaaaa gcatcaaata gggagtattg tgccaaatta tatggtattt
    14461 gattaggtgt aaaatacgac cctagatacc agattccaca aacaagccct aaaataattg
    14521 aagagcctat aaatccaaat ctaactaata atttgtcaat ggcgtatcct ctccatccag
    14581 cttcttgact aagtatactc aatataaaac ttactaatag catataagct atttgagaag
    14641 gtctaagtat tactatttgt ctccaatcta tatcagaagt ttttacatta aagtagtttc
    14701 cgattgttag agaagtaata aaggttaaag caaaaaataa tattgttaat aatggccatt
    14761 ttattcccat ttctttaaag cttaggtatc tataaatgaa gtcttgtttt gcactttgtg
    14821 gatagattgt agaaataaaa aatagaccta caatagctgg tgatgtatac gcaagataaa
    14881 aaatgatttc tccaagtatc gtatttgtaa ttccgagtaa tgcaggtgta agactgaata
    14941 tccagttcaa tactagagta agtgtaaaga aatagattaa atttttgttt gtattaaatg
    15001 gggtgttata tagttgcatt ttttcactat cctttcgttt tataatggaa tttgaatttt
    15061 tattgattgt ttatttattt ctatatgtat tgagttatcg tttaatgtta gttattgatt
    15121 ataataatat cctattctta ttttgattaa ttgtttttac actaaatata atcaattaat
    15181 atttaatatt gaacatatta tattattttt aattattaaa taggtcaata aatttgcaat
    15241 tagtgatatt aataaagtac tttacatttt tattaaatat ttagcttttc tttaatattt
    15301 tttatataac aacgatttat aataactttt tcgttgtttc ttaaagtagc ttgcatacga
    15361 ttatgtgata ttacttttat actttttatg tagtccatat ttaaaatcat ggatttggat
    15421 attcgtgaga aggatgtatt agctaataat tttgataatt tatatatttt taaatcaatt
    15481 tcaaatacat catcttcata ataagcaaat gttttatttc caactgtctc aaagtataaa
    15541 atatcacaga cattgagctt ataaatctct cctgattttt taccctttat atcaagtaat
    15601 ggtgtactca ataatgaaat tattttttca atgttattat ctagagtaga acattttatg
    15661 gttatttcaa tatcatcgca attaatgcct tgttcgacat gtattttcat taatcttcac
    15721 ctcattttat tactgtcatt agaatagcat tatatcttat tgagtccaac tagatgcgtt
    15781 ctaaatttca aattaagtat agtgaaattc aaaaatgaaa cggtgaaatt caaatttgag
    15841 gtggtaaaat tcaaaaaaat actatggata tttgggtaaa ttatccaaat atcctagtat
    15901 ttttcataga atatatctaa ctattttaca aatagactta ttttatgact tacatatatt
    15961 gtcaaattac ttcttaaata gcaaaatatt tttggatagt gtatcaatac tgtttgataa
    16021 agtatttaat ttaccctcaa ttctaaccaa caaatacatg gacagagcta taggaaaacc
    16081 aacattggtt attaacagct gtaaactatc atccataaaa ttagccccct attcttataa
    16141 ctaaatcata aggagtggta tttgtttcca caactttggc ttcaacaaca gaagctaggt
    16201 caaatcctcc aactgaaaaa atatttttag atactatcaa atccatgaca gattttatat
    16261 tttcttcagt taaatcatcc tttggatttt ctatagaaaa agaaacttgt ttatccttta
    16321 catttttaaa actcatgact aattttttct taatttccat ataattacct ccgatataat
    16381 aatttatatt ttaattgttt gattgttaat attaattgtt tatactagtt attgctgata
    16441 tagaaaatgt ttaatttatt tcaattaatt agattgaagt agggtagcat ttaaaatagc
    16501 taacatgttt aagttgaaat acgtttaata tattaatttt taaagtatca atatattaaa
    16561 gttataactc aaattgaaat tataacctgc caaatctaca gcgtattaag tgcgttacat
    16621 aactaaatct tttatataaa gagttaattt tttaatttgc aaatcagatt atgtttaaaa
    16681 gacactattt agatagtgat gtgctatcta attttaaaat atctacaata gggttctctt
    16741 gaagacttgc tattgcttct gcaacagcat aaacatcctc atttaatgcc tctgctttta
    16801 cgtttgcaaa tgatttactt ttaataactg ccttaccctt atcgtcaaaa ccagcatcca
    16861 attttagttt tatactagat aaattttttg ttgaaacaac ttccataaaa ttacctccta
    16921 aaaatttaat ttcaaagatt tgtcgacttt ctttgttagc tataatacta tcatctaaga
    16981 taatatcgga gttaaacagt aatttctaaa gattttttgt gtaaattttt ataggaaaaa
    17041 ttttccttat gaacaataat aagtttacaa attattcatg aagatatgtt tttagcgttt
    17101 tgaaatagta tttgtatata ttcctaattt agttgggata gttttgtata gaattttata
    17161 tgtatagagt taagtgttag aggttaaagt ttgtataaaa ttaaatgtta aagtctatgt
    17221 taaaaatttt attaaattta atctgtaaat aaaaaagtta aaagtccaat attaacattt
    17281 tacttttaaa ccattaaaat ctttattaaa gctaacaaag ttataagtta taagaaattt
    17341 tattaaagtt aatacagaaa ttaattttaa agctaagaaa ttttttgtta ggtccaataa
    17401 aactaataaa tatagtttag tcaataatgt attaaacaaa aactttgtta agtctataaa
    17461 aatatattaa aggaggtgta atttatgaag ataaaagtgg aagatatacc agttcaattt
    17521 catgcaatgg tagaaataat cggaattgat aagttcatag aggttaccaa gttgtatgga
    17581 ggaacaaata cttatatacc tacgtataat ggacttttta gatatgctag aaatagggaa
    17641 atagtaaaac aatttaatgg agtcaactac aatgaattgg ctattagata taatatgtgt
    17701 gtaagcaata ttaagcgaat actcaatgaa aatagtgttt aagtataatg aaaatagttt
    17761 tattaaatgt aagtgtaatg ataaagaaga tgttttataa agttttttta gacaaattaa
    17821 ctaaatttac tacttattaa tttaaataca ttgtatattc tgtaaaaaaa tttgttagat
    17881 actgaagata cattgagaat acaaagaaaa tagtcgaaaa tatttgttgg ctaaaaaata
    17941 gataaaatga tacaattgct tgtataaaag ggaaaaataa ataaataaac tatataaata
    18001 aagaaatatc aattaatatg acataatata tttataatat taggaatata aatagatatt
    18061 ttaaggaatt agttgtgaaa ttagaaaata ttaaaaaaaa tatatattta tatttaaaaa
    18121 aatttgttat ttatataaaa atacttatct agttactagc aattattagt agaaatgggg
    18181 gaaatagaga tgaataatgg attttacaaa ttaaattcag cagtattgaa ctttgatatt
    18241 aagagtgatg ttaacaagat aagatttaat cattatataa tgagaaaaga aaacatacaa
    18301 agacttcaag gaactctacc aagaggacaa ttttatatga cagtgagaag agctgctaca
    18361 gatttacaac tttctatttc tactataagt aggcttgtaa gagagttttt ggatttgggg
    18421 ataataagat tagtgtctaa gggtgtaaaa ggtaatcgtt ctgtatatag ttatgtaagt
    18481 atagagttta atggaagttt atttgatgat agcaaacata tggaacttga ttgtgtatgt
    18541 aatgatgaat ttgttgataa tagtgacata gagcttgatt gcaatagtaa agataattct
    18601 tttaatggag atataaatgt agaaactgag tattgtagta atgagtattt gtttaataat
    18661 gatgtagagc ataattatga caatttctat aacaaagata ctataaagaa taaatccatt
    18721 tttaatatta gcactatcaa aaaatatatc aatgaatctt ttgatacaaa taaatcagaa
    18781 aattctaact tacaacaaga atcaataaat tttaacttta atgatacatg taatgcaata
    18841 gataaaagaa cgaataatag tataaaaagt aatcaaaata aaaaggctat gaaattgagt
    18901 gacatcaatg ttttaagtga taaagatatt acaaataaaa agaattactt taatcatact
    18961 catggcatac aaaaaggcac taaatctgta acaaagaaaa aagaatcctt aaataaattt
    19021 aataaaaaaa atatacagca aagcattata aatatgctaa ataaaaaatc aggtaagaat
    19081 tttaaaatcg attctccaat aagcataaaa cttatcaatg aaaggctaaa ggagggctat
    19141 gtcttagaag atttctacaa agttattgag gtaaaagtgg ctaagtggaa aaacaccata
    19201 atggaaatgt atataagacc agaaaccttg tttagctata agtttgagtc ctatgtaaat
    19261 gaaaacatat ctatgaaaag tcaaagtaat attcacccgt gtaaagaagg caatagtaat
    19321 gatagcagta attatataca ttctaattat acaacgtatg atgacagtgt gcgcgagaga
    19381 aaaaaatatg aaaatgtaag atactggaat cctatatgtg aaatttaatt agtctaagaa
    19441 tgtatttatg ataaataaga tgttaatagc gaatagtgag ctactgcaaa tgatatatca
    19501 ataactagta gtgaattata ttgaatgata tatcaataat tggtagtaag ttatatcaaa
    19561 taagatatta ctatcgagta gtgaattata ttgaacaata tatcaataat tggtagtaag
    19621 ttatatcaaa taagatatta ctattgagta gtgaattata ttgaacaata tatcaataat
    19681 tggtagtaaa gtatatcaaa taagatattg ctattatgta gtaaattata taaaacaatg
    19741 tatcagaata aagagtatat taataatttg tagtatatca tgacgaataa tatagtcata
    19801 acaaatagtg tgtcataaca aataatgtat ttataattga taatatattt aaaaatcaaa
    19861 atataaaaac ttatgaggaa agtgagatta atttatatgg gtaaatcaaa agatgttgaa
    19921 atagagaagt ctattttagg cacaattctt ttagacaata aattaagcta taagctagat
    19981 gaattaaatg aaaacatgtt tatgaatgat atatgtcttg agatatttaa aattatgaaa
    20041 gaacttaaaa aagaaaacat agtgattgat gtggccactg tgaaatcaaa gatagataaa
    20101 aaatctctag caataaaaac gagtgatgtg actaatataa ttacttgggg tcagaatttt
    20161 gggcttgatg gtcatattaa aattttaaaa gaaaatttag ctagaagaag cataaatcaa
    20221 acctgccaaa accttttaca cagcctaaat cttggagaaa atatcgatac ttgtatttac
    20281 aaatttgaaa gtaatataaa ggagattttg gacaaggata cttatgaaaa tgatgatgta
    20341 aatagtatag cagggaaggt gcttgatttt ttagaaaaca aaaaagatat tggatttaaa
    20401 tttggtataa aattacttga taccactata ggaggtcttt ttaaaggaga gttaactaca
    20461 atagcagcta aatcaggggt tggtaagact gctttggcac ttcaaataat gttaaattca
    20521 tttaagcaag gaaaaaagac tttatttata agtagagaaa tgaccagtga gcaagtattt
    20581 atgagaaata tatgcagagt aactggtgta agtacaagga atatgaaatc taaagaaata
    20641 gatgaaaatg actggaaact tattgtaaat gcgataggtg atttaagcga aaataactta
    20701 atttatatca atgataaggt agatacaatt agtgcaatta gaaagaggat aaggcaggta
    20761 aaaccagatt tattgatagt ggattatgtg caactcctta catctcagaa aagcatggat
    20821 aaaagagaaa gggaggtagc tactttttcg agggaactca aaaatatgac tttagatttt
    20881 aatatacctg taattcaatt aagtcagtta aatgatgaaa tgaaagattc aagaccttat
    20941 ggagatagac ctatgaggga tagtaaagcc atctaccacg atagcaacaa tgtggtttat
    21001 atacatcaat taaaggggag tgattatgaa gaagcagtga gagatattgg agaaagtgag
    21061 gaggcagtta gagcatcaga atacagggga attaagatgg ttgatttaat agttgcgaag
    21121 tgtagagatg gacagactag gcataagcat ttttgttact ttggggataa gttacatttt
    21181 caagagctaa attattaggt caataaagta gtaataacaa gcttattaaa agtcgggaaa
    21241 tagcaaatag attataaaat atgaggtgaa aatatgttgg aattaacaaa aattatagaa
    21301 ttacttgaag aacacaagat agaagtaagt attccaaagg agttacataa gtatagttta
    21361 ttggaatacc ttgaattgtg tcaacaaagt gggggaacat atttttatat taagacactg
    21421 gaaggggctt taaaagaggc tagaaatgag cttattatta aattgtttaa tgggaagaat
    21481 tatatggaat tagcgaggat gtttgaaatg agtgttatta atgtcaggca tattgttaga
    21541 aaaaaataga tatttttgga gaatacaagt gtgatatact tctgaaatag aagttattat
    21601 aattgaaata aaattttggt taatagatta aagttgtgta taagtttaag attatagagg
    21661 agtataagtg tgattaacag catgattttg tgaataaaat tgaggatata tatgagctaa
    21721 atgtgtgatt aataaggtaa ggttgtgtat aagattatga gtatgtaaaa ggatagtcta
    21781 taattaatag ttcagagttg tgtgtttggt taagattata taagaattta atagattaaa
    21841 ctatactaat aaggtaaaat cgtgtgttta gttaaagctt attataaaat cttgatacat
    21901 gattaatgat gtaatatgtg aataaagtta agattataat aggtttaaat tgtgactaat
    21961 agaataaaat tgtgaataaa gttaagattg taataggttt aaattgtgac taatagagta
    22021 agagtgtaaa cagagaaaaa cttcaatatg agcttaactt gtgattaata gagtagggtg
    22081 taacaaaaga aaaatttcaa tatgagctta acttgtgatt aatagagtag ggtgtaacaa
    22141 aagaaaaatt tcaatatggg cttaacttgt gattaataga gtaaggtgta actaaagaaa
    22201 aacttcaata tgagcttaat ctgtgattaa tagagtaagg tgtaaataga aataaacttc
    22261 aatatgagct taacttgtga ataatagaat aagattgtga ataaagttat agttttagat
    22321 gggtattaac ctgtgactaa tagaatatga ttaggtgtaa aattatagag ttgtataaga
    22381 aaatattata gatatattaa atattagata ataaatattt atatagatac acaaaagaaa
    22441 aggcgatatg aagttaaact taatgacttc atatcgtttt ttatttgaaa ttcctctgta
    22501 tttagagtgt gatttttaat attgaggttt tatgtgatta agtcacagat aaaaagtaag
    22561 tatcctgata taattcttat agattaataa tattggggag gttattatgt ctaataaaaa
    22621 aaagaagtat gcaaatattt tagcaaatga gtgcgttgct tgtggtagct gtataaaagc
    22681 atgtccaaga agtgctatct cagttccatg tggcattagt gcaaaaatta atagagattt
    22741 atgtgttgga tgtgggattt gtgaaaagat atgcccagca tctgtaattg agattataac
    22801 aatattaaaa gaggaagaag gtaagtgtca tgaataataa aaaacattgg tatgactatt
    22861 tatggatttt ttcttcagta tatttgatat tgggatttgt aaatattatt ttcgcttgga
    22921 ttggactaat ttgtttcttt gttccactag ctatttctat tgttaaggga aataaagctt
    22981 attgtaataa gtattgtgga agagggcaac tgtttaattt gcttggaaat aaattgaagc
    23041 tgtcaagaaa tagagatatt cctaaattta ttagaagtaa gtggtttaga tatgggtttt
    23101 tgacattttt tatggtaatg tttgccaata tgcttttttc aacttattta gtatttgaag
    23161 gaagtagaaa ttttaaagaa gtagtgactt tgttgtggac tataaaaatg ccttggcaat
    23221 gggcgtatca tggaacttta gtttctccat gggtggtaca gtttgcattt ggtttttata
    23281 gtgttatgct cacatcaact gttcttggtt tgattactat gatgttgttt aagcctcgtt
    23341 catggtgtgt ttattgccct atgggtacta tgactcagat gatatgtaag gcaaagagta
    23401 agtctgtata gaaagaattt atgtgatatt taagagtttg ttcaagtaga gatgagtagt
    23461 aggtttgaat atatgacaaa tatatgagta taggggcata gatttagttt gtgtaaggta
    23521 aactgattta tgcttctttt ttatgtctta gattattggc taagtggaaa taattaattt
    23581 agatattaag aattattcag ttatttgaag aaaatataat aattaacatt atatgaatgg
    23641 atattcatta catataagcc agttaatagc aagatttatt taatatgaag taggtcaagt
    23701 atttaaacta aataagatca tgacaattaa tataagtata gatataaaaa gattaaaagt
    23761 tataacttat aaaaggtatt ctatataaat agtatgggaa aaatagtcaa attaacttat
    23821 tagaaacaaa aaatatacat aaaaaataaa tgtaaatatc aaataaggta ctatatcaaa
    23881 atagatataa gtatcttttt tattattgaa attatgaaaa tatatttgat tttacacaaa
    23941 aaagagtgcc tcatgaacta aaaagttcat tttgagacac acttttacag tgtcaaagaa
    24001 cgaagtttat ccttatccaa tacttttata ccaccacgag ataaagacac aataccttct
    24061 ctagcaaaat atttcaacat tctcgataca acttctctgg cactacccat atacttagca
    24121 atttgttcat gagtcatatt tatagtgcta ctaccatttt tagctatctc atcaattaaa
    24181 aaagatgcca aacgcttatc aaaactcata aaaagaattt gttgcatagc ccacataaca
    24241 tcagaaaaac gttcagtagc taatttatat gaaaataact ctgcatgaat attttctgta
    24301 gataattttg caaaaacaga ggaactaatc tgtataatat cgcagtccgt ttcagcatct
    24361 acatagacat caaaagttat tgtttttaga atacaggaag ctgataaaat acatacatct
    24421 ccatcatcaa gtcggaacag tgtaacctct cgaccttcat ctgaaagtat gtatgtgcgt
    24481 attgttccag actttataat caaaatacca atacagtcac tatcaccaca atgtacggga
    24541 ttcccttttt tgtaatgcga ggtgtttgct gaagaaatta ataattcttt ttgtaagtca
    24601 gtaagtttat cccaaaaagg caagctatgt gtaagtatat caatttcatt ttgtgaaatc
    24661 ataccatatc tcctttctta aattgttata atcccatgat acaagtgaat agataaattg
    24721 acaagtagtt tgttaagagg attatttagt cagatagtaa tagtatgatt tatgtagaaa
    24781 aaatatgata atatatattc ttgctattaa tgaaaaagtg atatattttt gatgatagta
    24841 gatgtgtaca actattgcat acagtttatt tttaaaagat taagttcctt atgtaacctt
    24901 atagacataa aacctgcaat tatcgtagct atcaaatcag cgattggttg tgcatataag
    24961 ataccattta tcccataaaa taatggtagg taatcccata gataacaatg cttttgggat
    25021 tggggcagac cctaataatt caattctttt gttactttct ttcatattca acacttccct
    25081 tctattgaca ttaagtctaa attataatac tataaattat agtgaatatg gaaaatagta
    25141 ctataattta tagtatttgt caatagtaag aaaactaaag gagatgattt ttatggctac
    25201 aatagactta attgtattgg gaatagtaaa gcaacaaccc aagagtgctt atgatattca
    25261 aaaggaagtg gaatacagga atatatcacg ttgggttaaa ataagtacac catcaattta
    25321 taaaaaagta attcaattag aacaaaaggg atatattgaa agtaaaactg tgaagaatgg
    25381 aaaaatgcca gaaaaagcag tttattcatt aactgaatta ggtgagacac atttcttaga
    25441 cttaatgttt gatacatcta gtaaaccaat aaatattctt ttagatttta atgctgttat
    25501 agtaaattta aatagcctta caccagaaaa acagaaatta tgtctcaata atattgaaga
    25561 gaatgttcaa gagttaaaga aaactattga ggaaaatata agtataaaga cagaagtacc
    25621 agagacagca aaagcagtgt tggagcaaca gttggtacta gcacaatcat tggaaacatg
    25681 gataaattct ttaaaagata atatggggaa ttagaattag agaagctaac ttaataatca
    25741 ttaaacaaaa accagcatat ttaataatga gatattagaa aaaatgaaac aaatgatata
    25801 aactgaaaag tatatttaaa ataaagctca cttaattgag ggtgaatgtt aaactttcta
    25861 agtgtaagga ggcattattc aggaatctac tcgactactg ttattgttaa tggtgtagaa
    25921 tcaggtaacc ttaattctgg attaggcata tgatttgatt ttgaattagg tgtataattt
    25981 tgttaataag tagaaaaatt ggaatctgta gtcgtggttg taaatttcac tttatcattg
    26041 attaacatag ctgttgatag aatatttcaa aaattagaat ttggagagtg agcactatgg
    26101 aagaaaaata catcttatct gaccgaggga aagtatatta ttggataaaa gaaagtaata
    26161 gtgtgtttgc aaagaactta atttttttgc caggactaac agcaaatcat catctttttg
    26221 atttccaaat acagtacttt tcaaaaaatc ataatgtatt ggtatgggac gctcctgctc
    26281 atggaaaatc aagaccttac ttagatttct catattctaa cttggtagaa gaattgaaag
    26341 taattttaga taaacaaaat atgaaaaggg ttgtacttat agggcaatcg gcagggggat
    26401 ttgtggcaca gtcctttatt gtaaaatatc caaatatggt agaagggata atgatgattg
    26461 gaacttgtcc atatggaatt gactattata gtaagttaga tattttctgg ttaaaacaga
    26521 ctgaatggat gttaaagttg ttcccagata gattgttgcg aaacataata tctaagatgt
    26581 gtggagctac gtctgaaggc agaaggagta tgctgaaaat gttagaagga tacgaaaaaa
    26641 aagaactgtg tcatctcatg tatctgggat ttgctgggct tataccagaa attcaagact
    26701 taaatataca ttgtccagtt tgtttagtag ttggagataa agacaagact ggtaaagtaa
    26761 gaaaatataa tgagcagtgg cacaataaga ctggatatcc attgcacatt ataaaaaatg
    26821 cttcacataa tgtaaatgtc gataaaccta atgaactaaa tcagattatg gatatattta
    26881 ttaagcaatt ataatatata aaaattacat acccattagg taaattatgt agtatatgtg
    26941 tttatagatg gttatagaaa taaatatgta ataaactact taacacttaa aaagtagact
    27001 tgtctagtgc ataatacaaa gcatgatata tatttttaag cgaaatacat taaaagaaga
    27061 actagtggag ataaaacaga tattagatat tataaatgat taacttaaaa gtactataag
    27121 tgtagagaaa gatgactcat acgttattaa gggattgtaa atgattagtt taaaagtact
    27181 atcgtatagg attctgcata tttagaccct agtaaagata gttaaagtag tttaaaaact
    27241 aaaaaattat ataaaggaat taggtataag aaaatgattg agagtatacc agcaaaacaa
    27301 attttacaaa aagtgaaatt tgacaataca agatggtttg gaattgacta taatatgaac
    27361 ctctatcgtg gttgttctca tggatgtata tattgtgata gccgtagcac aatatacaac
    27421 atagagaatt ttgacaaagt tagatataaa gaaaatgtga tagaaatttt aagtaaagaa
    27481 ttgcgttcaa aaagaaaaaa gggggtagta ggcattggag ctatgtctga cacatataat
    27541 ccttttgaaa agcaactttg tattacaaag aaagcactag atttaatttc ggaaaatcat
    27601 tttggagtgt ctattgatac taaaagtagt ttggtggtta gagatatacc gattttgcag
    27661 aagattaaaa aaaataactc tgctattgta aagttgacta taaccactgc aaatgatgaa
    27721 ttatcaaaga aaatagaacc ttatgtaaat ccaagttctg tacgatttga ggcagtgaaa
    27781 gaacttaatg attctggaat tttttgtggc atattattga ctcctatgct tccattttta
    27841 actgataata aagatgaaat acgagcaatt gtagaaaaag cacacaaggc taatgcaaaa
    27901 tttatttatt gcatgtatgg tgtaacaatg cgtagtggtc agagagagtt tttttatgag
    27961 catttaagag atatatcacc aaaattggta cttaaatatc agaaaacata tggtttaaac
    28021 tatgtatgta ctgtacaaaa taaagatggt tgtgagaagt tgttaagaga agaatgtgca
    28081 cgttatggta ttttgacgga tatgaaagag attataaaag cgtacaagag gtcggagtca
    28141 tatactcaaa taaaattttt ttaagataag ctttggataa gatataatag tgatgattga
    28201 tgtgttgata ctctaggtaa ttactagagc tgacattata attgaatatt agtattgata
    28261 ttctaggtaa ttgttagtga tgatattata gatgaatatt aatattgata ctctaggtaa
    28321 ttgctagagc taatattata gatgaatatt agcattgata ttctaggtaa ttgttagtga
    28381 tgatattata gatgaatatt aatattgata ctctaggtaa ttgctagagc tgatattata
    28441 gatgaatatt aatattgata ttctaggtaa ttgctagagc tgatattata gatgaatatt
    28501 aatattgata ttctaggtaa ttgctagagc tgatattata attgaatatt agtattgata
    28561 ttctaggtaa ttgttagt
//
LOCUS       DAMJVM010000041        28124 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.042, whole genome shotgun sequence.
ACCESSION   DAMJVM010000041 DAMJVM010000000
VERSION     DAMJVM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28124
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.042"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(156..2756)
                     /locus_tag="PC523_001954"
     CDS             complement(156..2756)
                     /locus_tag="PC523_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDF2475479.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLYKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSSKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKVKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARMH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLLNTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISERLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDAIIIEETYVKPRENVLEETPAGNVFIEEPGYYILKVR
                     INIQLPALGYKVVSFESSDKEMVSLNKSNDSFISNDSYKVIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDEDIKLSFDRAYTEKALGYEKLVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVIIILSKENPLMDVKLCINNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIKDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQIFKKLEFSFAIYMDSKS
                     FDELEIANFTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIINKTVSRELEIISLPENY
                     LISACYPSYYSKDKFVIRLENQSSKRMILDEQIFKNINGKIVNAIEDVEEEQLYEIAP
                     FDVISILLDL"
     gene            complement(2786..3901)
                     /locus_tag="PC523_001955"
     CDS             complement(2786..3901)
                     /locus_tag="PC523_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2475480.1"
                     /translation="MEGNVMLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGS
                     FYNIPDIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPG
                     LVGGMIAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLIT
                     VVLINYVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTS
                     LALLTSGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSE
                     GAIPFAVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSI
                     VVGTVIVAGLVIVLRGNPVIEDDEFEDDDFDESEWEA"
     gene            complement(3902..4222)
                     /locus_tag="PC523_001956"
     CDS             complement(3902..4222)
                     /locus_tag="PC523_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2475481.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            complement(4225..4686)
                     /locus_tag="PC523_001957"
     CDS             complement(4225..4686)
                     /locus_tag="PC523_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2475482.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHRIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            complement(4686..6650)
                     /locus_tag="PC523_001958"
     CDS             complement(4686..6650)
                     /locus_tag="PC523_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2475483.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIASDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLVRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIEILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKEEYIEESLYIGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVAADQHRDTNELKNTSTNPCETNTNNVSNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDNECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSGCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKCFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            6816..7670
                     /locus_tag="PC523_001959"
     CDS             6816..7670
                     /locus_tag="PC523_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar uptake protein"
                     /protein_id="HDF2475484.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMVSGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVSRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKENKNKKEWFSILIGIMLIMSG
                     VFMLSIIK"
     gene            complement(7912..8379)
                     /locus_tag="PC523_001960"
     CDS             complement(7912..8379)
                     /locus_tag="PC523_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HDF2475485.1"
                     /translation="MKTKEEYLKEVRTTAEECFRRGEFFCSEAVLHTINNALGQPLSP
                     EITKLASGFPVGLGKAQCLCGAISGGEMALGLVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRVITKKWDGDNFMSPERKEHCIKITGQVAEWVADKLIEDEKLNIK"
     gene            complement(8483..9415)
                     /locus_tag="PC523_001961"
     CDS             complement(8483..9415)
                     /locus_tag="PC523_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDF2475486.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGVIGFILY
                     AIYIIKIVLLRKGVKEQLENPLISSTFPTITMATMIFATYVKPLSLELGIMIWSIGVV
                     GHLVLIVLFSKKFLIKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLINGHLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIHLSKSETTSKAK"
     gene            complement(9584..10528)
                     /locus_tag="PC523_001962"
     CDS             complement(9584..10528)
                     /locus_tag="PC523_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HDF2475487.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMICDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELARKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGITLEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRGDAKISIRDMKTIKVDKNKQISIFD"
     gene            complement(11168..11842)
                     /locus_tag="PC523_001963"
     CDS             complement(11168..11842)
                     /locus_tag="PC523_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2475488.1"
                     /translation="MNDELKTILYSKGVDIVRFVDISEFPTSQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTGSDEFLEKEEKVEGLADWISKYIQSKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RHPLIDSKCLDCNICVENCPAKAIHGNEWTLPGERESIIDVSKCFCVLRCMMSCPWSL
                     RYANQK"
     gene            complement(11902..12648)
                     /locus_tag="PC523_001964"
     CDS             complement(11902..12648)
                     /locus_tag="PC523_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDF2475489.1"
                     /translation="MKIENLSINDIKRGYIFNDDLNCYSCIKCTKIYEIGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQMINSDSKYNTLTENQKQLFQLFISDLPDKEIAKKVG
                     VSTSTIRHQKFMFREKAKQAKLYLALYESVFEDKSNKGSTIISIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKQYSEKEVNQILES
                     IYGDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            complement(13126..13812)
                     /locus_tag="PC523_001965"
     CDS             complement(13126..13812)
                     /locus_tag="PC523_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2475490.1"
                     /translation="MAILELINVNYKYEGSSKFVLKDVNAEFEIGKMYVIVGKNRAGK
                     STLLSVISGLDDCTSGSIIYKGEDLKNINKNIYRSKYIGSIFKNHNLLNDATVLDNIV
                     LTMLIAGNKAKNKEDIVYDILKKVGIDKEKANCKVKDLSQDDIRRVCIARALSNNPEL
                     IICDDIIENTDDKGEKSLVDIFYKLASEDNKCVIITTTFDRIALYADELWGITNGNLM
                     FVKSLEKLKY"
     gene            complement(13823..15181)
                     /locus_tag="PC523_001966"
     CDS             complement(13823..15181)
                     /locus_tag="PC523_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2475491.1"
                     /translation="MYILNNAIKNLIRNKVHSLFSIVIILLMVLSIAISSIINLNMSI
                     ITQKYEKKLESEVSIKIDEKKVVQNDLTESLIGYDPLNLEDYLNFSKSKYVKKMSLTG
                     NLALYVDNKKYAPSNDKKLDYNDITTNCIVMGFTDKNKSLKYYNLNISKGRVFQKDNE
                     CIVSEDFLKAGNFKVGDTISLLNDYNTNVKLNLNIVGVYKTKDSNKLESVQDVSIYNN
                     ILTSYNTLVTFEKRVRPESKMIYTSASFLLRDSNSRKAFEKELRNKGLSDIYKVTIDE
                     LLYHKLMAPIERLSGVVFTYTLGILVLGSILLVISSAISIKKIKYDICVLRLIGMSKF
                     KIIRSFIYESIMIILISTVLGLGIASISEKSISNYVFEYQYKYNITYNDFLFNKGSYK
                     DVNTNDINTEQEMKNLKDKSTLNFILKIIFISMILILLTSTVSIYNAIMCKPIQIIYD
                     RN"
     gene            complement(15243..16289)
                     /locus_tag="PC523_001967"
     CDS             complement(15243..16289)
                     /locus_tag="PC523_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2475492.1"
                     /translation="MQGLKDKKTIALILYSVIVFIIFTVGLKYIINISDIDKNLISRD
                     TTLSKSEIPLNNSYVLDKGLMTNYNLILKNNFNVVIILFFAFIVTSNFILLLILNRLD
                     FKERIEILNNINNIEDENEILSIDPIMSRVYKDLKNKFDSHIEDYKRLNSYLSHEQKN
                     AISILRTSLELDGNKELLNVLDKVSNSVEDVLAISYIKNNEDMYEVDIALICAQVCDN
                     YSKVYSNLKFDFDEDENMSIFGREKWIYRGVSNLLDNAIKYGEGKEIYINVKNQKGSV
                     IVSVKDNGIGMNQHHIDKIFYDRYRINELNKDGYGIGLSLVKHVCNLCNGFIWVESEE
                     NKGSTFYLVFKEYK"
     gene            complement(16277..16948)
                     /locus_tag="PC523_001968"
     CDS             complement(16277..16948)
                     /locus_tag="PC523_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2475493.1"
                     /translation="MRVLIIEDNKELSYNIKSGLENAGFVVDIANCGLDGEEKAFVND
                     YDAILLDLNLPDKDGIEILEFFRSNNLNTPVIIVTARDGVNELAKGLDLGADDYIVKP
                     FQIIELRARIQAVIRRFYGRTNPNIESGPITINPSSRKVYVYNEEVTLGTKEFDIVEY
                     LASRYPDIVSGIDISEHVYDEYFDPLSSVLRVHIARLKKKLREVAGKEIIITTRGIGY
                     SICKD"
     gene            complement(17335..18219)
                     /locus_tag="PC523_001969"
     CDS             complement(17335..18219)
                     /locus_tag="PC523_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2475494.1"
                     /translation="MRYDIAIIGSGPAGLAAAINAKIRNKNIILFGTEDISQKLIKAP
                     SVENYLGISNISGLELKEKFKNHIDDMGIKITQNRINNVYSMGEYFTLVSGNHVYEAT
                     TVVLATGIEYGKMIKGEDAYLGKGVGYCATCDAMLYRNKKVAIIGYNYEAEEEANFLS
                     EIASNVYYIPMYKLENKINSGINVIDDKPLEIKGGKLVNKLVLRKSELDIDGVFIIKD
                     SISPKYMVPGLEVEGSHIKSDKNMNTNIEGLYVAGDCAGKPYQYLKSAGQGQTAVSSA
                     ISYLDKQKIKEFMLVDAH"
     gene            complement(18385..18702)
                     /gene="trxA"
                     /locus_tag="PC523_001970"
     CDS             complement(18385..18702)
                     /gene="trxA"
                     /locus_tag="PC523_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF2475495.1"
                     /translation="MIKIINHNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     DEMAEKAKFLKVDIDQSIELAQKFEVSTVPTMMIFKDGKPVDRLIGFMPKDNLKNKVE
                     SYM"
     gene            18954..19706
                     /locus_tag="PC523_001971"
     CDS             18954..19706
                     /locus_tag="PC523_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HDF2475496.1"
                     /translation="MNDIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYVPISPNELLKNKKEEFNNSMDKLKNNLQILNKG
                     KNIEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKNLEGLDNIHIHGGNKTTLENKMLYKGIILIVDNNEILTGNLSEGNEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQD"
     gene            19693..20574
                     /locus_tag="PC523_001972"
     CDS             19693..20574
                     /locus_tag="PC523_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDF2475497.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTNASNISDEIKQNVAKEMN
                     LSETAFVSESNVADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLDSNKSIFYQETK
                     AGVLSVELKEINNENIFMMEQNTPEFENLDNYRKELAQMIGVHEDRLLEYPIMKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVNQNILDKTSFIGEEGNFINRPGKVYVNIAKENNEITS
                     VKIGGSAYTVLSGEIIY"
     gene            complement(20801..22156)
                     /locus_tag="PC523_001973"
     CDS             complement(20801..22156)
                     /locus_tag="PC523_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2475498.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYSAKFLIYIFLTTAV
                     IHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDNQEELDTAFSVQYSFVNIGSFI
                     GTTAVGILYAKTFAKNGVLGFSQCFFIAAVLCVIGAIWFTYGWRFLGNAGKRPFKEGV
                     VAEKVEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVDMTIG
                     GFDIPLAWFDSLNALVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFTRGVGAPETAKASIMWIIMFGILLSFGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGQGYGYIYAFTLKFEMIKVYTIIPVILFVAAILLFLFDKTLNRLVEDD
                     K"
     gene            complement(24018..24527)
                     /locus_tag="PC523_001974"
     CDS             complement(24018..24527)
                     /locus_tag="PC523_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HDF2475499.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLNPI
                     YSNNTIIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
     gene            complement(24738..26147)
                     /locus_tag="PC523_001975"
     CDS             complement(24738..26147)
                     /locus_tag="PC523_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475500.1"
                     /translation="MENYFDKQAKELYDKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMLREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKSELPWNLYL
                     NRMMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKAEIAVALDISGSISDEEFKQAI
                     KEVLSIVKNYNHEIIIIECDNEIRRVYKVKTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVETNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            complement(26151..27281)
                     /locus_tag="PC523_001976"
     CDS             complement(26151..27281)
                     /locus_tag="PC523_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2475501.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LVAIDGNLLKEGEIGGLPTIESYVGVNSSGYKTEKKTTVYAVHNKLREIDEEISKGKI
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKENNNSIPRAVFLNVIKGNVGKVIAEEFISFIESDSKPLISYEEV
                     FLGESIDESIVERVKKESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIKFLKMY
                     PVDLMIGIMKDIRNSYTEVYKKAIENEEFVKSYFESYSLIRG"
     gene            complement(27293..28096)
                     /locus_tag="PC523_001977"
     CDS             complement(27293..28096)
                     /locus_tag="PC523_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475502.1"
                     /translation="MEKSRVIEMIKNNPNIISTIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEEMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYVKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYSAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYIVKKISKDELEEVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRHSENIPIDKIMFIYKYGSKTAKKIAVDEKLKIV"
BASE COUNT         9514 a         4657 c         2825 g        11128 t
ORIGIN      
        1 taaattgttt atcctgttaa actaattatt ctacttctat aaattctata ttaaataata
       61 aatccataca ttaacattta cccaaattta tccaaattaa aaagtctggc agattaatat
      121 ctgtcagact ttaaacaata aacttacgtt tacacttata aatccaataa tattgaaata
      181 acatcaaaag gtgctatttc atataattgt tcttcttcta catcttctat tgcatttaca
      241 attttcccat ttatgttttt aaatatttgc tcatctaata tcattctctt actactttgg
      301 ttttccaatc ttattacaaa tttatcttta ctataatagg aaggataaca tgctgatata
      361 agatagtttt ctggtaaaga tattatttct aattctctac ttacagtttt attgatgatt
      421 gatttttgaa tcttattgtc tatacgatat aaaaagaaat tataatcctg taattgatag
      481 tttacacatt cccttagata attgtgggta aaattcgcaa tttccaattc atcaaatgat
      541 ttagaatcca tatatattgc aaatgaaaat tctaatttct taaatatttg agctttatct
      601 gttttaattc ttatatgacc ttttttagtt gtatcaccag aagcacgtcc tggtctatat
      661 aataaatcag gctttcctag ttcatctgta gtagcaagca aggtcaatgc aatttcatct
      721 ttaatatgtt gatactcttt tattcctcta gtaaatacag tacaacttct attttggttt
      781 gtcagcgtaa tattactttc caaaggttct aaatctatag gcatctcact gtaaatatct
      841 tcccagttgt ctaaaactcc attttctctt gttatatatc caaatggcaa tgatgctata
      901 tttttattat cttttatatc agtttttata tgaacacgca atctatgatt atatatcgtg
      961 ttattaatac aaagttttac atccatcaat ggattttctt tggataatat aataattact
     1021 tttacattta atttaccatt aagatatttg ttttttctat cttctaatgt aagcggtagt
     1081 aaagtagtac ctttaactac caacttttca tatcctaaag ctttttctgt atacgctcta
     1141 tcaaatgaaa gtttgatatc ctcatcattt tttaatggag aaaaatcata ggtgtcacct
     1201 gcattaccac tatcttttaa tgttaaaaag tttgatatgt aagttccatc tttaagttgt
     1261 aaatttaaac ttccatcttc atagattacc ttataactat cattagatat aaaagaatca
     1321 ttagatttat taagtgatac catttcttta tcacttgatt caaagctaac aactttataa
     1381 ccaagtgcag gaagctgtat atttattctt acttttaata tataatatcc tggttcttca
     1441 ataaatacat tgccagcagg tgtttcttct aatacatttt ctcttggttt tacataagtt
     1501 tcttcaatta taatagcatc taaattatct ttaaaataaa tatttttact atcacttaca
     1561 acttgtatct ctttatatcc attaaagcgc ttggcttctg tattaaatac taaaatgtca
     1621 tttttagata atttaagcct ttctgaaatt tttttgacaa tagtattttc aatactgtca
     1681 catatttcat ttgcttctct tatacggtga agaatatcat ctgttacagt atcagttaca
     1741 catccagcta aactatcgtg tgcttgccct tccaaaagtt ttttccatgt atttaaaagt
     1801 aactgattgc ttatttcaat tttactcttt ttagcaatta ctagtaatgg ttctattctt
     1861 ttaatcagct tgttttctag tttatcacat gctaatttta tatccattct actagaacca
     1921 attgatttat gcattcttgc caatacaggc tctctaaatt cacctctata gctttctagc
     1981 gattctatgg attttatata ttttaaaaac tcttgatatg aactcgttat atatgaatac
     2041 tttccgatat cattaatcgc tttaacttta tttttgaaat cacctattat atttagctgg
     2101 tcatttccag aaggtattaa cacctcatct acatcagtat atgatttaat aaaatcaata
     2161 gctgggtcta agcgtccatt aacatacttt gatgaaggtt ctaaaagcat accagttcca
     2221 tatccatgtg gcatatttac tgcatatata taactttctc catttaagct tttccactta
     2281 aaatatggtg attgaacctg tttaccaaga tgtatacctc tccaaaagaa tatattatca
     2341 agacctacat gctttaatag tataggcatc tgtgcattaa atccaaatgt atctggcaaa
     2401 tatccaattt tcatatactt accatacttt ttactttcaa aaattccaat cattaagttt
     2461 cttaaaatag actctcctct agcaaagaat gcatcagttt gagtaaacca tggtccaata
     2521 tgtagtctat cttcttttat caacttttta atgatttcta ttttttcttt gtataattgt
     2581 acataatcat ccaaaataga aagttgtcca tcaagtacaa aacttacatc tgggttacgt
     2641 tctaattcat caattacatc tgtaaatagt tgttcactaa gtaccaatgc atctccagaa
     2701 gtaaaatacc attctctatc ccagtgtgta tgattaacaa tatgtgcttt tatcatttat
     2761 tctcctctgt tctttatctt taattttaag cttcccactc tgattcatca aaatcatcat
     2821 cttcaaattc atcatcttca atcactggat ttccacgcaa tactataact aacccagcta
     2881 caattactgt tccaactact attgataaca catacattgg ccaattctct actgtaaacc
     2941 atccataaaa tccactaatt ggagttgcat ttactgaacc taaaccaact gccatagctg
     3001 aagcaattgc agttccaatt acgcatgaag gtattacttt taatggagat tctaccgcaa
     3061 aaggaattgc accttcactt actccaatta accccattat aagagatgac ttcccagctt
     3121 cacgtaactg tttattatat ttttgttttg ctagtatagt agcaagtccc aatcctagtg
     3181 gtggtataac tattcctacc attgcagcag tattaggtgc ataaattcca cttgttaata
     3241 atgcaagtga tgttgtcaaa gctgctttat taactggtcc acctaggtca actgccatca
     3301 tagcccctat aatagccgcc attaatatct gatttgtacc agataatcca tttaaccaat
     3361 tttctaatcc agtatttaat gtggcaaaag gtatacctat cacatagtta attaatacaa
     3421 cagtgattaa tgttccaaat actggtacta taaatattgg tactattgaa gctgctgatt
     3481 ttggtaattt gacatgattt ttaataaata aacatgtata tcctgctata agaccaactg
     3541 ctaaagctcc taaaaatcct gcacctatat ctttagcaat cataccccca acaagacctg
     3601 gtacaatagc taatttatca gctatagaaa atcctataaa tgcagcaatt acaggaaaca
     3661 ttaatcctaa tgcagtacct ccaaatccat ctaaatcatg caataatcta acaatactac
     3721 tagcactttc tgcatattta gcatcccata tatctggtat attatagaaa gaaccaccta
     3781 cacgagatat tgccattatc atagcacctg caattactag tggaatcata tatgagattc
     3841 cagttaagac atgctttttt aactcagaga ctatcttttt caacattaca tttccctcca
     3901 tttatttagt gtcaattaac tttaatgcat catttattac tttctctgca tcctttatag
     3961 gcgcattaac tggtacttct agtatttctt ttccctcaaa acgttcagca tttctgactt
     4021 tagtgtctac tgcaaatatt actacttctg caatttgtgc atcctttgta gttagctcat
     4081 tatcgattcc tgtagctcct tgtgtttcaa cttttataag gtgacccatc ttttttgaag
     4141 ctttctttaa tgcttgagct gccatatatg tatgtgcaac tcctgttgca catgctgtaa
     4201 ctgctataat ttttcttttc atctctacat ctctccttct tcactaaata tattgattat
     4261 ttcttgttct gaattggcta attttagtgc atctacattg tcatcatcca taagtttagt
     4321 tgccaattta gataatagta caagatgatt gctatcctta tcaatattgc ttatagccaa
     4381 taagaaaatg ctatctactg gtttttcatc taaagcatcc cactcaattc tatgcttagt
     4441 ctttgcaaat attatagaag atttcttgac agcattactt tttccatgag gaatagcaat
     4501 accattacca atacctgtag ttgaatgctc ctctctttcc aaaacagact taacatattc
     4561 ttcttcattg tccacaatcc cattttcaac aaatatacta cttaattttt ttattgtttc
     4621 ttcctttgta tttgtatcaa gattaaaaac aatttgttct tttttcaata catctgatat
     4681 tttcactata tactctcctc cactagcaaa tcgtacactt cctttgtaga agttgcactt
     4741 aatatgtcat ttatattttt tttattgtca atgaagtcat aaaaaacttc atatatttca
     4801 tcaagctcta ggtttttgtc attttctaac gaaataaaga aaataacttc taccttttca
     4861 tttccccatg taataggatt ttttaacgtt gcaacagata tattagattt tttaatataa
     4921 ctaggattag catgaggtgt agctattgat tggaaagatg tgtaggataa attttctctc
     4981 tcaattgcac ttttagctat accttctttt gcaaaacatt tttttattag gttatctgtt
     5041 atatacttaa ttgcatcttc ataatttgag gcttcaatat ttaacaatat attttcttca
     5101 aagattaaat ttctgaaaga tttatacttc ttagaagtac aacctgaaac attttgtata
     5161 tttctttcca aggttcttat atcactttca tttaaaatag ggctaactac tatcgttgga
     5221 atagtttcta gctctaaata aatagtactt attactaagt ctgcatcaat tttactagat
     5281 actaattctt gtacagaaag cactttttca atctttatca ttggaaagta tttttttatt
     5341 cttgcagaca ataacctaga tgaacctagc cctgtactac atactagaat tactctaata
     5401 ttttgtggaa gttctttcat tctttctcta aaagcttcaa aatgtaatgc tatataagca
     5461 cattcattat catttatcct aatatcatat ataaattcaa ttactttctt taaatctaaa
     5521 gcttgctcaa atgataaaga aaagttttgt ttgattgtat ttacatatgg atttttaata
     5581 ctaagtccat atttcaatct atttattgat gaatgtacat gtgaagtcaa tccatcaatt
     5641 aaatccttat cataagctgt ctcatacaat gtatttttta taatttcccg aagatttgat
     5701 acattatttg tatttgtttc acaaggatta gttgaagtat tttttaattc attagtatct
     5761 ctatgttggt cagctgctac taaatgtaaa tttatatatc caatttcaga ttttggtaat
     5821 aatattgaaa atttttcttc taattgtttt gctaaataat tggtatttga catttgatta
     5881 ttttctatat taccaatata caaactttct tctatatatt cttcttttct aattctctct
     5941 acagctattg ccaaatgaat aactaacgac tgaaattcat aatcagagaa ctcaaatttt
     6001 ctttctctgc tgaaattcct aagaatttca ataattattg ataaatcatc ttcagaaaaa
     6061 ataccattta catctgcctg tatcttctca aatgcttgat aaaagctttc ttgctcttgc
     6121 ttaatatacc agtttctact ccaaaattta tgtataaact tagcagtaag ttgtctttta
     6181 tctctttcac ttatatttaa ctttattcct ttactaggtt tcttatatat ggaaatccct
     6241 tcatttttta acaatttttc gacttcggct aaatcccttt caatagttcc acgacttata
     6301 tataattcgt cagaaagttg ttgtatagtg atatagtcat tactcttcaa aagtactgaa
     6361 tatatataga taactctctc ctctctggtt gtaggaattt gacttccttt attatctagg
     6421 taaggaagta ttttttttaa atctccatca atataaacac ctatcttagg ttttcttact
     6481 aaagaaagac ctatcttttt aagcactgct tctacttctt taagagattt tgaaactgtt
     6541 ttttctgaaa cgtctaaatc acttgctatt gcttttacag ttgtatgacc ttcttttaat
     6601 aaaaacttta ttattttaat ttcacgattt tgaatcatat cagttaacat tttcaatttt
     6661 actccttccc tttgtcttta ttctaatgga aaatgttttt ctaaaaaact actaatattt
     6721 cctgattaaa tacggtaatt tttatgtatt gaatacttta attttagatg ataaattaaa
     6781 aactgtctaa atttaatatg aaaggaaata gtttaatgct gacaataatt attgctttac
     6841 ttcctgtaat gggatgggga ttgatgccca tagttgcaaa tctaaagaaa agttctcctc
     6901 atgaacaact tttaggtaca tccataagtg cgtttatatt ttccactata ataactctag
     6961 ttatacatcc tgaaattaca tttttttcat tttgtataag tatggtttct ggcatattct
     7021 ggagtttagg acaattaatg caatttaaag caattcaaat tgctagtgta tctaaagtaa
     7081 tgccgatttc aaatggttca caattaactt ttacgacatt actagctgta atcttattta
     7141 atgaatggac aagttctaaa atgttactga ttggttcttt atcaatatta tgtataatac
     7201 ttggtatact tctcacaaat tatcaaacta aaaaatctac agataaaaat atgttaaaat
     7261 gtgttggagt tgtattatta tcatctctat ttttatcatt atatgttgta actaatcaaa
     7321 tatttttaat tgaaggatat agaattatat tacctcaatc tgttggtatg ctgataactt
     7381 catctattat tgtcaaatat ttttctaaag agattgtatc cagggaaaac gtcttattca
     7441 atttaattac tggaatatta tggtcaatag caaatttagg tatgttcata acgactaata
     7501 cattgggagt tacaataagc ttttctatct ctcagagttg tgtgatagta gccactttag
     7561 gagggattat attttttaag gaaaataaga acaaaaagga atggttttct attcttattg
     7621 gtataatgct aatcatgtct ggtgtcttta tgttgagtat tataaaatag gtatttattt
     7681 acacaaatca tatacatttt tatacatata aattaactgt ttctatttat taataaattt
     7741 acctatatac ttaaaataat agcatatgta tttatgaaat taaactatac tctaaatgta
     7801 agtatatttc attaaataca tatgctataa atttttctgt caataagaga gtacaaaaca
     7861 tcttatattg taaataaatt taactttata aatttaccaa ctaatattgt attatttaat
     7921 attaagtttt tcatcttcta taagcttatc tgcaacccac tctgcaactt gacctgttat
     7981 tttaatacag tgttcttttc tttcgggaga cataaagtta tcaccatccc acttctttgt
     8041 aataactcta caacatgtcg caccatattc ttttttaata taatcatgca accctttagc
     8101 atgttcaaac atctttggat tcatagcttc accatgaact cttccatata ctaaacctaa
     8161 cgccatctca cctccagata ttgctccaca aagacattgt gcttttccaa gtcctactgg
     8221 aaatcctgat gctaacttag ttatctctgg acttaatggt tgtcctaaag cattatttat
     8281 tgtgtgaaga actgcttcag agcaaaaaaa ctctcctctt ctaaagcatt cttctgcagt
     8341 tgttctaact tcttttagat attcttcttt tgttttcatt ttttccctcc aataaaatta
     8401 agctaaatta agtttctatg gtttgtctta tgttcgtata tttatataaa tattcctacc
     8461 aaagacacat atttaataat tcttatttag ctttagatgt agtttcagat ttactaagat
     8521 gtataataaa taaaactaaa acatataaaa taatcactgt agcaattatc tcttctaatt
     8581 tagaagcata tgaaatccaa cttataaaat gacctgattt agttaaatga ccatttatta
     8641 gtttcatccc cattgcactt attgccatag ggaatgtaaa tgcagcaaaa ctcggataaa
     8701 aaggcaatct taataatttt ggtaaatatc ccaagactat aatataaaac acaattgata
     8761 atataaataa caagtataat actacattat ttttatctga aaaagaatta atatatccag
     8821 acaataatag tgaccctggt gcagctaata tagctatact tggtaaagat ggttcaggca
     8881 tttccttgat tatccatact ctcttcaaaa ttactggtat aagaactata taagatacaa
     8941 atcctatcca aaaagcaatt ttgccaatca aaagttgatt aaatgctgga gctgtaacac
     9001 tagctgcaac tatcccaaca tagactatat accaagatgg aaatactgtt tttattgaga
     9061 atttaattag gaatttttta ctaaatagga ctattaacac aagatgaccc acaactccta
     9121 tgctccaaat cataatacct aattctaaag ataatggttt tacatatgtg gcaaatatca
     9181 ttgttgccat tgttatagtt ggaaatgtac ttgatattaa gggattttct aattgctctt
     9241 taacaccctt tctaagtaat actattttga ttatataaat tgcatataaa ataaatccta
     9301 taactccaat tacattacgt aatgattcac tataagattg taataagtta cctaaagtaa
     9361 atattgacaa aatcaaactt gcaataggta taggatattt ttttaatagg ttcaaattat
     9421 gcctcctttg ttactaaatt atttatgttt taattatatc aaaaagatgg tcaaattgac
     9481 atattatgac catctttctt atatagaaaa tatctatcaa atttaatttt attattataa
     9541 ttatctatat tcttaatata ttaagttaat tagtatattt attttaatca aatatactaa
     9601 tttgtttatt cttatctact ttaatagtct tcatatctct aatgcttatt tttgcatcac
     9661 ctcttaaagt atctatgagt aatttattat cttctttata aagcctacag ttttcttcta
     9721 cttttgcata attaaagtta gcataacaat acagacaata atgtctgcaa gtattgtatt
     9781 gccctatatc gacacttttt acacatccac aaatatctct ttgtgtacta tcttttttta
     9841 cctctacttc acatcctata atttttgata ttaatctatc atcaatacac ttactttttc
     9901 caatcccaaa tttacttaaa ttatattctt cagagcacat ttcaagtgtt atcccatgct
     9961 tatgagcaat cttagaaatc tcttttgata atgtttctat ttctgtaatt tctattggct
    10021 ttatatgtaa tgctttagta ttaaattttg ttttcttata taaatcgata aaacttatta
    10081 tacatttttc tgtaaatcca tcaagctgat tacataaact cgcaaatgct ttgatatgat
    10141 aatctactgt gtacttttca cttaaaaaaa tcgggtcata ccttaaaatt actctttctt
    10201 tgcctatttt tctagccaat tccttaaatg aatctattac ctttctttta tctaaaatgc
    10261 aggtctctac ttctttgcca tatgatgtta tagtatagtg gaaataatat ttatagtctt
    10321 ttaatttatt taaatcacat atcattggag tcacatcctt tgtccaaaat acaaaacaat
    10381 caacagtatg tggatttaac tctatcttac taacttgctt tggattcatt ggattaatta
    10441 cataaacaaa accttcttta agtctgttaa aaaaccactt actatagaaa gcaggtatat
    10501 cagttcttct actcacactt actatcattt ttaacctcca tagtaatata attatgctct
    10561 aatatatttt taatataatt atagctctaa atacttaatt taatcaaaac atttaagata
    10621 attccagtat gatttactga ttcatttcta gcatttaaaa tatccttaat aagtttacaa
    10681 ttctttaaaa ataaatatat attattccta aacttctaaa actaatttta taatgaaact
    10741 aaaaaaggtg ctattttcaa gcaccctaat ccaatctgga gttttagttt caatttccat
    10801 gattaattcc tcctttttaa atctaaattc ttctcacaga aacaattttg aactataact
    10861 gaagtgcctt ttactatctt tgggaatttt gagaatagca ctttcatttt cacaatatta
    10921 ttataatttt attatatttt tttataatct cattatattt tcactattac taaattactc
    10981 ttaatttttt aatataaatt ttgtacaatg attaaaatag ttaagcttta tatcaatata
    11041 atctaatccc aataaaaaaa acactcattt ctgagtgttc tatacaagtt tttattttga
    11101 gacatcttat ataatcttat attttaaaaa gtttataata tccaatttaa tatcttcttt
    11161 agtaaagtta tttttgattt gcatatctta gtgaccatgg acaactcatc atgcacctca
    11221 atacacaaaa acactttgaa acatcaataa tagactccct ttctcctggc aaggtccact
    11281 cgtttccatg tatagctttt gcaggacagt tttctacaca aatattgcaa tctagacatt
    11341 ttgaatcaat caaaggatgt ctttcaacag aaattggagc gtctgtaagt actgtacaca
    11401 tactaaaagc acatccatat tcctctgtta caaacaaatt atttttcccc ataaatccaa
    11461 taccactaat atttgctatt gtcttatgtg gcaaaggact aatccctgat tgcatttctg
    11521 gattaatata agctttttca aaatatccat gttctagatt atttttctct gattgagaat
    11581 aagctctata tcctttactt tgaatatatt ttgaaatcca atcagctaac ccttctactt
    11641 tctcctcttt ttctaagaac tcatcactac ctgtaggtaa gttattgtat atatctgtaa
    11701 taaatttttt tgataaccct atacaaaata aaattgcttt agagaaaccc tgtgtttgac
    11761 ttgttggaaa ttcagatata tcaacaaaac gaacaatatc tacaccttta ctgtataaaa
    11821 tagtctttaa ttcatcattc atgtttgact ccttctatag gtaaaaacaa ccttatatat
    11881 taataaaatt aactattcta attatgttaa ccaatacttt gtacattctt tatttctact
    11941 cataaaacca tactcaatta aatatcttct aattgttgca aaatcaccat atatagattc
    12001 taaaatctga ttaacttcct tttccgaata ttgcttccca cgttcaaatt gttccaaaat
    12061 cttagataga attacgacct ttttcttttc ttttgaagaa aatactttta attttaaagg
    12121 attcatactt tcaaatgatg tatctaaaat gtgttttctc tcttcttcag ttactacata
    12181 tctttcatct accattcgag catgttcatg aattgatatt atggtgctac ctttattaga
    12241 tttatcttca aacacagatt catatagtgc caaatataac ttagcttgtt ttgctttttc
    12301 tctgaacata aatttttgat gacgtatagt agatgtagac actccaacct tttttgcgat
    12361 ttccttatca ggtaaatcac taataaacaa ttgaaataat tgtttttgat tctcagttaa
    12421 tgtgttgtac tttgaatcac tattaatcat ttgctctaag taatcactat gttctaattc
    12481 catatgaagt tcaatagctt ttgaagcttc aaagaatcta ttacctattt tatatatttc
    12541 tcctatttca tatatttttg tacatttaat acaactataa caattcaaat catcattaaa
    12601 tatataacct cgtttaatat cattaataga aagattttca attttcatat tactgtctcc
    12661 atttcatatt ttttcatttt tataaatata tcatatcaaa gtttattaat ataatcaaca
    12721 tttttaattt ttgtttataa aatataatta tttattaatt taaaatcaca atatatttcc
    12781 cacctttaat atcattacct acattattat gtattcatat tgtgatttga atgattagtt
    12841 attgtaactg gagtaataag tgatatcccc tttaattcaa tcttagagtg gtcaattttc
    12901 aaaattggag ttccttcttt ttaaatttac atcagctaaa tcaaaggcta tcattagttc
    12961 taattaatat ttaacatttt atgtaaaatt tataaaaaaa tgtatataaa ttaagataag
    13021 tgactatact aaaaatatga tacggacaaa caataagttc aacctatctc ccccaaaata
    13081 aaatgagcta cagtttcccc caactgtagc tcattttcat gtaatttaat attttaattt
    13141 ttctagactt tttacaaaca tcaaattacc attagttatt ccccataatt catcagcata
    13201 taaagcaatt ctatcaaaag ttgtagttat tatcacacat ttattatctt cagatgctag
    13261 tttataaaat atatctacca aactcttttc acctttatca tcagtatttt ctataatatc
    13321 atcacaaatt attaattcag gattattaga taatgctcta gcaatacaga ctctcctaat
    13381 atcatcttga gacaaatctt tcactttaca atttgctttt tctttatcaa ttcctacttt
    13441 ttttagaata tcatatacaa tatcctcttt attctttgct ttatttccag caatcagcat
    13501 agttagaaca atattatcta acacagtagc atcatttaat aaattatgat ttttaaatat
    13561 cgaccctata tacttacttc tgtaaatatt tttattaata ttttttaaat cttctccctt
    13621 atatataata cttccactag tgcaatcatc aagacctgat attacagaca ataaagtact
    13681 ctttcctgct ctattttttc ctacaattac atacattttt ccaatttcaa attctgcatt
    13741 aacatctttt aatacaaact tactgctgcc ttcatattta taattaacat taattaattc
    13801 aagtattgcc aatctatatc acctaatttc tatcataaat tatttgtatt ggcttacaca
    13861 taattgcatt atatatagat actgtactag tcaataatat caatatcatt gatataaaaa
    13921 ttatcttaag tataaaattt aaagttgatt tgtcttttaa atttttcatc tcttgttcag
    13981 tgttgatatc atttgtattg acatctttat aactaccttt attaaacaaa aaatcattat
    14041 atgttatatt atacttatac tgatattcaa aaacataatt tgatattgat ttctctgata
    14101 tacttgcaat tcctaatcca agtacagtag atattaagat tatcatgata ctttcataaa
    14161 taaagcttct aattatctta aacttagaca ttccaattag tctcaacaca catatatcat
    14221 attttatttt ttttattgat atggcagaac ttatcaccag caatattgaa cctagcacta
    14281 atataccaag agtatacgta aaaaccacac cagataagcg ttctattggt gccattaatt
    14341 tatgatataa taactcatct atagtaactt tatagatatc acttagacct ttatttctta
    14401 attctttctc aaaagccttt ctactattag aatctctaag taaaaaacta gcacttgtat
    14461 aaatcatttt actttctggt ctaacccttt tttcaaacgt tactaaagta ttatatgagg
    14521 ttaatatatt gttgtatata cttacatctt gaacactttc taatttatta ctatcctttg
    14581 tcttatatac acctactatg tttaaattta acttcacatt agtgttataa tcatttaata
    14641 aagatattgt atctccaacc ttaaaattac cagcctttag aaaatcttca cttactatac
    14701 attcattatc tttttgaaat actcttccct tacttatatt taaattgtaa tacttaagac
    14761 ttttattttt atctgtaaat cccatgacta tgcaatttgt agttatatca ttatagtcaa
    14821 gttttttatc attactaggt gcatattttt tattatcaac atatagtgca aggtttcctg
    14881 taagtgacat tttctttacg tattttgatt ttgaaaagtt taaataatct tctaaattta
    14941 aagggtcata tccaattaaa gattctgtta aatcattttg aacaactttt ttttcatcaa
    15001 ttttaatact aacctctgac tcaagctttt tctcatattt ttgagttata atggacatat
    15061 ttaaatttat tattgaggaa atagctattg ataagaccat taataatatg ataactatac
    15121 taaatagact atgaacttta tttctaatca agtttttaat tgcattattt aaaatataca
    15181 ctttatatct cctcttatta attaaattct atattaaata tttttataat tcttattaac
    15241 tcttacttgt attctttaaa cactaaataa aaagttgaac ctttattttc ttcactttca
    15301 acccatataa acccattaca taaattacat acatgtttta ctaaactaag acctatacca
    15361 tacccatctt tatttaattc atttattcta tatctatcat aaaatatttt atctatatga
    15421 tgctgattca ttccaattcc attatctttt actgatacaa tgacacttcc cttttgattt
    15481 tttacattta tatatatctc tttaccttca ccatatttta ttgcattatc caaaagatta
    15541 gatacacctc tataaatcca tttttctctc ccaaagatac tcatattttc atcttcatca
    15601 aaatcaaatt tcaaattgct atacacttta gaataattat cacatacttg tgcacatatt
    15661 aaagctatat caacttcata catatcctca ttatttttta tataacttat tgcaagtaca
    15721 tcttctacgc tatttgaaac tttatcaaga acatttaata attccttatt tccatcaagt
    15781 tctaaactcg ttctaagtat tgatatagca ttcttttgtt catgtgataa atatgaattt
    15841 aaccttttat aatcctcaat atgagaatcg aatttatttt ttaaatcttt atatactcta
    15901 gacataatag ggtctatact cagtatttca ttctcatctt caatgttatt tatattattt
    15961 agtatttcta ttctttcttt aaaatccaat cgatttaata ttaataaaag tataaagtta
    16021 gaggttacta taaatgcaaa aaatagtatt ataacaacat taaaattatt cttaagtata
    16081 agattataat tagtcattaa tcctttatct aaaacataag aattgtttag aggtatttca
    16141 ctcttagata aagttgtatc tctagaaatt aagttcttat ctatatctga tatatttata
    16201 atatatttta aacctactgt gaaaattata aaaactataa cagaatataa aatcaacgct
    16261 atagtttttt tgtcttttaa tccttgcata tagaatatcc tatccctcta gtagttatta
    16321 ttatctcttt acctgcaact tctcttaatt tcttttttaa tcgagctata tgtaccctta
    16381 atacagatga taaagggtca aaatattcat catagacgtg ttctgatata tctatacctg
    16441 acacaatatc aggatatctg cttgcaaggt attctactat atcaaactct tttgtcccta
    16501 aagttacttc ttcattatat acatatactt ttctagagga tggatttatt gtaattggac
    16561 ctgattctat attggggttt gttctaccat aaaatcttct tataaccgct tgtattctag
    16621 ctcttaattc tataatttga aatggtttta ctatataatc atcagctcct aaatcaagtc
    16681 cttttgcaag ttcgtttact ccatctcttg ctgttacaat tattacaggt gtatttagat
    16741 tattacttct aaaaaattct aatatctcta ttccatcttt atctggcaag tttaaatcta
    16801 ataatatagc atcatagtca tttacaaatg ctttttcttc tccatctaat ccacagttag
    16861 ctatatctac tacaaaccct gcattttcca aacctgactt tatattataa gaaagttcct
    16921 tattatcctc tattattaat actctcatat aatcatctcc taaataaaat atcagtttat
    16981 ttattatata taatgaatgt ttattgaatg ttaactttat tttataagta tttatatttt
    17041 aaacaaaaca caattttatt attattcaaa tgcttgtatt tgtcaaattt aagatgaatt
    17101 tatctaatca tttagttatt atcagcaatt taaaaaggtg aattcatcta atagaaccca
    17161 cctttttcaa ttaaatcttt attttgaaat agcatttctt agtattgagt gtatgtatta
    17221 aaaaaacaat ccctgtaatc tttattaatt ttaaatatat ctttcagtat tgaatccaca
    17281 atcaataaca acttataatg atttattgtg gatataaaaa cttcatagtt ttatctagtg
    17341 agcgtcaaca agcataaact cctttatttt ctgcttatct agataagaaa ttgcactgga
    17401 tacagctgtt tgaccttgtc ctgctgattt taaatattga tatggtttac cagcacaatc
    17461 tcctgccaca tataaacctt caatatttgt gttcatattt ttatctgact ttatatgaga
    17521 gccttctact tctaatcctg gaaccatata ttttggtgat atactatctt ttataatgaa
    17581 aacaccatct atatctaact cactctttct caatactaat ttattgacta gttttccacc
    17641 ttttatttct aaaggtttat catctattac atttatacca ctattgattt tattttctaa
    17701 tttatacata ggtatatagt atacattact tgctatctca cttaaaaaat ttgcctcttc
    17761 ttctgcttca tagttgtacc ctattatagc aactttttta tttcgatata acatagcatc
    17821 acatgttgca caatacccaa ctccttttcc aagatatgcg tcttctccct ttatcatttt
    17881 tccatattca atacctgtag ctaatacaac tgtagttgct tcatatacat gatttccaga
    17941 aactaatgta aaatattcac ccatactata tacattgttt attctgtttt gagttatctt
    18001 tattcccata tcatctatat gatttttaaa tttctcttta agctctaaac cacttatatt
    18061 actaattcct agataatttt ctacagaagg agcttttata agtttttgac ttatatcttc
    18121 agttccaaat aaaattatgt ttttatttct aattttagca tttatagcag ctgcaagccc
    18181 tgctggacca cttcctatta tcgcaatatc gtatctcata atctctccta ttttcaataa
    18241 cctttccttt tttatctaaa taatatatcg aaagtcaatc tcattagcaa ttggatactt
    18301 atttataacc acacaaactc ttatactata taaacaaaaa aactcattat aatgagtttt
    18361 ttgtttatat atgatttatt ttaattacat atatgattca actttgttct ttaaattatc
    18421 cttaggcata aaacctatta atctatctac aggttttcca tctttaaata tcatcatagt
    18481 tggcactgtg gatacttcaa atttttgagc tagttctata ctttggtcta tatctacctt
    18541 taaaaatttt gctttttcag ccatttcatc tcctaaagct tcatatattg gtgataacat
    18601 cttacaaggt ccacaccaag ttgcaaagaa atctaccact actaatccat ctttattttc
    18661 tacttcgttt ataaattcat tatgatttat tatttttatc ataatttaca tctcctttat
    18721 tattttcttg atattaatat actacgacct gtgtaatact tcaattagtc acttttttgt
    18781 aaccttctat ttttgctggc tttaatatta tgataagcat atttataaga ttaaatgttt
    18841 ctaatatatt ttaatttttt cattaatata gttgacattt tttactttag attataaaat
    18901 caaattaata actacatagg tagttactaa acttttatat aaggagttta tctatgaatg
    18961 atataattaa tgaattacaa aatataggat ttactaaata cgaatctcaa atatacatat
    19021 cattattaaa ggaaagccct ttaacaggat atgagataag taaattatca ggtgtaccac
    19081 aatcaaaagt ctatgaaaat ataacaaagc tattaaacaa taatataatt atcaatgttg
    19141 gaactgaccc tattaaatat gtacccataa gtcctaatga attattaaaa aataaaaaag
    19201 aagaatttaa taattctatg gataagttaa agaacaactt acaaatatta aataaaggta
    19261 aaaatataga atacgtttta aacataaagg gagataaaaa tatattaaaa aaagcaatag
    19321 aaatgataaa aaatgcaaaa caagaaatta taatttcttg cccttatgaa gaaattcttg
    19381 aattaaaaaa agaactccta attgcttaca aaaaaaatat taacattgaa atactacttt
    19441 tagaaaataa aaatctcgaa ggtctagata atattcatat ccatggaggt aataaaacta
    19501 cacttgaaaa taaaatgctt tacaaaggaa ttattttgat tgtagataat aatgaaatat
    19561 tgacaggaaa cttatctgaa ggcaacgaag ccatttcaat ttggacaaaa aactctaaca
    19621 ttttatatat tggagttcaa tacataaaac atgaaattta tatttcagaa caaataaaag
    19681 gaggttataa aaatgaacaa gattaagatt aaacaagttg atgcttttac aactatacct
    19741 tttggtggaa accctgctgg tgtagttact aatgcctcta atatttctga tgaaatcaag
    19801 cagaatgtgg ctaaagaaat gaatttatca gaaactgcat ttgtttcaga gtcaaatgtt
    19861 gcagatttta aggtacaatt tttcacacca ggtttagaag ttgatttatg tggtcatgca
    19921 acaataggta ctttccatgc acttttggaa gaaggaaaac ttgattctaa taagagtata
    19981 ttttatcaag aaacaaaagc tggtgtatta tctgtagagc ttaaggaaat aaataatgaa
    20041 aatatattta tgatggagca gaatacacct gaatttgaaa atttagataa ttacagaaaa
    20101 gaacttgcac aaatgatagg tgtacatgaa gatagacttc ttgaatatcc tattatgaag
    20161 gtaaatactg gactttggtg gcttgtgttt ggcttaaaat ctttagatga tttaaaaaat
    20221 atctctccag atttaaataa aatcaaaaaa tttagtggtg ataataatat aattggcata
    20281 accccatttt gtatagaaac tatagataaa aagtgtgact accatttaag atctatcgca
    20341 ccatatgttg gagttacaga agaccctgta tgtggaactg gaaatgcttg tgtatcatct
    20401 tatatagtaa atcaaaatat tttagacaaa actagtttta ttggtgagga aggtaacttt
    20461 ataaacagac ctggaaaagt atatgtaaat atagctaaag aaaataatga gataacttct
    20521 gtgaaaattg gaggaagtgc ttatactgtc ttaagtggtg aaattatata ttaatctcat
    20581 ccctaataaa aacaaataaa aatactagct aactaatgct agtattttta tttgtttatt
    20641 tttatttaat aataatactc taatacatat tataatcaca tatattataa cattatattt
    20701 agaagtaaaa ttttattttt actattaatt agttataaat caatctaata caatataatt
    20761 acaatagatt aatccataaa acacaataat tacttactga ttatttatca tcctcaacaa
    20821 gtctatttaa tgtcttgtca aataagaata ataatatagc tgcaacaaat agtattactg
    20881 gaattatagt atacacttta atcatctcaa atttaagagt aaatgcatat atatatccgt
    20941 atccttgacc agctatgaaa gtagcaaatg tccatactcc cataagaaca cctaggagtt
    21001 tcttaggtgc atacttactt acaaaagagt taccaagtgg tgagaataac atttcaccaa
    21061 aacttaatag tataccaaac atgattatcc acattatact agctttagca gtttcagggg
    21121 ctccaacacc tcttgtaaac tctgccccaa caagcattaa aaatgcaaga cctaagaata
    21181 ttaacccaag acctgtcttt ttaaataaac tcatatctcc ttgtggtcta ctagcaagct
    21241 taaaccataa tgctccaaga actggtccta atactataca tacaagcgca tttaatgagt
    21301 caaaccaagc aagaggaata tcaaatcctc caatagtcat atctacaaat gctgcaccat
    21361 aatcataaac tgcaagataa gttagatacc agaataccca gaaaattaca gagaagaatg
    21421 atataagtat aatagcccaa attctcttct tatccatact tgtaagagga gacttatctt
    21481 tttcttcaac tttttcagca actacacctt ctttaaaagg tctctttcct gcatttccaa
    21541 ggaatctcca tccatatgtg aaccatattg cacctattac acaaagtaca gctgctataa
    21601 agaaacattg agaaaatcca agcacaccat tttttgcaaa agtcttggca taaagtatac
    21661 caactgctgt agttccaata aaagaaccta tattaacaaa agaatactgt actgaaaatg
    21721 ctgtatcaag ttcttcttga ttatcaaaaa gttgtccatt tactgcagac acatttcctt
    21781 taaagaatgc agtaccaatt gaaactaaga ttatcattgc atttaccata gaaactgtag
    21841 ttgcaataga accaagatag tatcctacac ccattataag catacctata ggtatagtat
    21901 acctagctcc tatccatcta tctgatatat accccccaat tataggtgca agatatgtga
    21961 atgcaacaag gttagcttgc attatagctg cttgtccctt atctattcca agtccaccat
    22021 gaattacagc tgttgtaagg aatatataga ttaaaaactt tgcagaataa tatgctgctc
    22081 tttcaaatga gaaagttgtt gagcagatat aaaatcccat aggaaattta cccttctttt
    22141 ttactgttga gctcataagt ttaaatcctt tcttaattat atttttatat ttttacgttt
    22201 tatccaaact ctttctttta atgaaagata tggttatatt attttgtaaa acaatttgta
    22261 attattatta agataattgc gcatttattt gtatagtata tattttcatg gaataaaatt
    22321 aatatacttc tttagccata aatttaaatt atataaatta cttgttttca tatatttata
    22381 aattagtacc ttaatcatct aataccatga aaaaaacttc gccatatgca attcttattt
    22441 ttttacgtga tagtatttta caaactaaaa tccccctttt gtagcaactt atgtaattac
    22501 attttagcgc ttttttaaag gagtctagaa taaagcaaca tatatttatt caaataatgt
    22561 ttttatttac ttttaaattt atatattact tcacccaacc aattctcaac ttaaataaat
    22621 aaactactat ctattcacag attatattta tcttttactt attagattta taataaacac
    22681 tgtgaatcta aacataaaaa actgttattt taaagtgatt acccatatat tcgcttcgat
    22741 actatttgtt aaggttatac tatttattta taatatatta ttcataaaat accttaatct
    22801 gaaaactttt tcacgaatat ttattttaca cctcccacta tttatttcac catgtaaatc
    22861 gtaccataaa tatatactta tatgcaagaa aaaaatctat taatcgacag taaataatta
    22921 gaaaaattta aattatattt gtcaagtata ttatataaaa tattttctat gaaaatataa
    22981 gttaaattaa tgttcagaat agttttttag atatgaaatc ttgattttct tattacttta
    23041 cttacttgct caaatatatt tttgtatgca gtatataata gtcaaatcgt acacttctta
    23101 taactgcgtt gatttgaaaa taatattaat agttaataga agaaaaaatt caaattaatt
    23161 tttaaaaaaa ctataagtta attgaatact tttttatgta cttaattagt tttataaatt
    23221 caattatttt ctaaatttag gtgtgattta ccatataaaa atgatttttt atcttaattt
    23281 tctaaatata agtgaatgct atcattttaa gtatcttata agcaatgtaa ctatatatat
    23341 ctaataacta cttttttaat atgcttaaac tatttataag caattatatt tatctgttaa
    23401 cctaataact ttttcactaa aaaataacaa tctattcaaa aataaactat agtgcttctt
    23461 ctactttgtt tcaataaaat actaaataaa tttcgtacat tcacaatttt tcaggctaca
    23521 ctataaatat acaatattta tcttagttat aagtctaaga atatttttag tcttattttg
    23581 tcgaatattt ttaataaaac ttcataattc taagcatatt ttttacactg tgttttcatg
    23641 tacaaaaata atacttacaa ataaacaaca taaataaaat ttaaataaga tggattctat
    23701 tcaacatagt atccatctta ttcattcaaa acctaaaagg attttttata atattataca
    23761 ttttaaaatc taaataactc attatagtat caatttcact taaagcttac aatacactta
    23821 ttaatcttac tttataataa aaagtataaa aacatctaca ataacaattt aaatctttaa
    23881 gaaattatat cttatagtaa ttcttcaata caatcaccct taatttttta tataatttaa
    23941 ttatatactt tattttacta gaactaatac acataaataa tacttaaaat agattaaata
    24001 aaggatacta aaacacttta ttgaggtata tgacttgaaa tatatgaact aacttcatta
    24061 ggagaaatat ctaaaatagt tgcaaattct tcatttaata acctttcagc ttctttcatt
    24121 atattatcat ctgcttgatg tgttttttta ccaatagatt ttacatactc tctgttattg
    24181 tctatgcttc ttactaattt tattaactct tcacatttac cacctctgag tattttttta
    24241 aattgctcac ttcttaattt ttcattatct atccatgagg tatccatatt aggaatatca
    24301 tttattaatg aaagtacatt aattttagaa agcaactttc tcattggtac tttttcatta
    24361 tctacaggaa tttttattat tgtattatta gagtaaatag gattcaatac ataatactct
    24421 ttttttattc catttgtaaa tcgttcattt gttatatcca tgactttgca gactcctgtc
    24481 ataccataca ttatataatc atcaatctta aacaaaaaca tcaccatcct taataaaaaa
    24541 aaaagttttg acaactaaat ccaccttgcc aaaactatct gctatatatt atatattaac
    24601 acattttaag actttaatgt aagtaaaact tatattatta aaattaaaaa atacatcaag
    24661 cattgattaa tgtatttttt agatttttat aacaagcata tcaaaataaa ttaagacagc
    24721 ttatctcctg taaataccta taaaattggt gcttggctat tcattgagta cccatcattt
    24781 cttacatcat ttatttctaa tatttcatca tttgtttcaa ctttagtaag ttttttaact
    24841 gctccataag gttcttttaa tgaaatttta tctcctcttc cagaaataac ccataaaatt
    24901 ttataccctc taggtataac ttgtagctta ttttcacctt taccatcagt aaagtagact
    24961 aacaagttaa tctttttatt attagcatac tcaaagactg gagaaaatct agtacctcca
    25021 cctatcctaa ttctatcttt tatatctttt tcagttttca ctttatatac acgtctaatt
    25081 tcattatcac attctataat tataatttca tgattatagt ttttcactat actaagaact
    25141 tctttaatcg cttgtttaaa ctcttcatca ctaatacttc cacttatatc aagtgcaact
    25201 gctatttctg ccttatgact tctaagttct cccctcaaat ctaaacgatt tggttgtctt
    25261 ctatttcttc ttgttatagt cttcttttta ttattttcaa ctgttcccat cattctatta
    25321 agatataagt tccatggcaa ttcactctta ctatttttaa gtgatgatat catactttct
    25381 aaataagcag gaactttacc tttttgagaa ctattaataa atttttcagt aaattctcta
    25441 agggtatttt catctatctc attagattca tcccatatat catgagttct ttctggacta
    25501 taatcaattt ctatatctcc atatttatca ctatcatctt cctcaccatc ttcatccact
    25561 tccatcaaat ctaattcagt ttgaatcttt tccacataat attcaaaagg ttcatatggc
    25621 tcaagtttta aggaatattt taaatttacc cactctaagg tagttgcata tggtggtaaa
    25681 ttgtttagaa atttatttac tactatatcc attgccatat taattgccaa tgtactatac
    25741 tttcctttta actcctttgc tctcatcaga tgcatagata atatatgatg aatttcatgt
    25801 ttaattgtag tctccatttg ttttatatca agcgttaaaa aaattattgg attaaaatat
    25861 ataacatatt tagccccctt aaaatttaca cctgtaggac tacttatatc aaatcttatc
    25921 tctcttaaca tctgaaacaa aaaatatcca taaaaattat cctcttcttc cataagactt
    25981 aaattcactt tatctacaag actaaaaaac tcatacttaa aatcctgtgg tatatctata
    26041 tcaaatttct cattagcacg atttgccttt aacatagaat gagtatttat aatttcactt
    26101 gctttatcat aaagctcttt tgcttgttta tcaaaataat tttccataca ttatcccctt
    26161 attaaactat aagattcaaa atatgatttt acaaactctt cattttctat agctttttta
    26221 tacacttcag tataactgtt tctaatatcc ttcataatac ctatcattaa gtccacagga
    26281 tacattttta aaaatttaat aaatctatta atatagtgat ttgattcata tttatcattc
    26341 ttaatattta gctctaaatc ttttagaata ttcatagctg atagataaag tcttgtatga
    26401 ctctcttttt ttactctttc tacaatagac tcatcaatag attctcctaa aaaaacttct
    26461 tcataggata ttaatggttt agaatctgat tcaataaaac ttataaattc ttctgcgata
    26521 accttaccta cattaccctt tataacattt aaaaatactg ctctaggaat agaattatta
    26581 ttttctttat aaactttata aattttagat actctttcat aacttctagg agtagctctt
    26641 acatcatctt catttatttt atgcagatat tctggaaaag tggaaataaa ctcgataact
    26701 tttctttcaa tcccttcatc tattgcccat tttatccatt gtgtatggtc actctccata
    26761 tttaaccaaa caaatctatt ttcttgagct gcatccatat ctaccacttg ataatcaaaa
    26821 tctgaaccat atttacttga tgggttcatt gccgctaaaa tctttacatc atcatgcaat
    26881 ttatatccat taatctctct atttaaaatt agattcataa gttcttgctg tactgtatgc
    26941 tcacaacgat ttatctcatc tataaataaa agaactatct tacctttaga tatctcttca
    27001 tcaatttccc ttaatttgtt atgaacagca tatacggttg ttttcttttc agtcttatat
    27061 ccactagaat taactcctac ataagattct atagtaggaa gacctcctat ttcaccctct
    27121 ttaagtaggt ttccatcaat agcaactaaa ctccaatcat tttctttggc aagcttattt
    27181 gccaaagctg tttttcctat cccactttca cctactatta aaggtacttc tccagtggat
    27241 aatattaagt caacgctttt taatgtgtct ataaaattca tttttatctc tcctaaacaa
    27301 tctttaattt ctcatctaca gctatttttt ttgccgtttt gcttccatat ttataaataa
    27361 acataatttt atctattggt atattctcac tatgtctatc tataacacta caatttaaaa
    27421 aatctctaat atacttctct tcaacatctt cttttgataa tacttctttt aatacttcct
    27481 ccaattcatc tttagatatt ttctttacaa tgtatttaat cactaagata ttcactttta
    27541 aaaattccaa cattttattc ttatctaaat cagttataaa gctaatagct gcttcattat
    27601 tttttatagc tataagttct gcactgtaac taggtgaatc tatatatctc aaggcatcat
    27661 aacttattct cacagcctcc tcttgtacac tttcataagg ttgttttata aactttatcg
    27721 aatcataatt ttttcttact gctaataact gtaactcttc acttggattt ttaacatatt
    27781 taattgccca acctgcttga tttatagcca tttcaataac tttatcagtt ggatttttga
    27841 tataatccag tatactccat ccctcattta ctgcttttat catcatctct tctgttggat
    27901 tatctataaa tttaattgct ctaatattat tatctattgc tagttcttgc atctctctag
    27961 ttggattatt tatatattct agtacaagtc cattttcttt aatagctaat aatttcattt
    28021 catcagttgg attatctata gttgatataa tattaggatt attttttatc atttcaataa
    28081 ctcttgattt ttccataaca tgcctttctt tatataatta tttt
//
LOCUS       DAMJVM010000042        27828 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.043, whole genome shotgun sequence.
ACCESSION   DAMJVM010000042 DAMJVM010000000
VERSION     DAMJVM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27828
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.043"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            152..248
                     /locus_tag="PC523_001978"
     tRNA            152..248
                     /locus_tag="PC523_001978"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:186..188,aa:Sec,seq:tca)
     gene            399..1106
                     /locus_tag="PC523_001979"
     CDS             399..1106
                     /locus_tag="PC523_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HDF2475503.1"
                     /translation="MENNFTTRTSFLVGDDGIEKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NQDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIINELLGQ"
     gene            complement(1248..2084)
                     /locus_tag="PC523_001980"
     CDS             complement(1248..2084)
                     /locus_tag="PC523_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2475504.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEYSKNIINSYLRQKRIKTIDIIIATHPDSDHIGSLDNVIKKFNVNSIY
                     MPEQSTNSESYQNLINSCTDKNLAIQYLYKDDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNNNSFLFMGDAEEENEKEILRSFELNNINFIKIGHHGSNSSSSLEFIKKIN
                     PDIAAISCGYKNQYGHPHKEVIDNLKQSHVSIYRTDRIGDIVFYSDGEIIFTKYNYEV
                     DT"
     gene            2271..3176
                     /locus_tag="PC523_001981"
     CDS             2271..3176
                     /locus_tag="PC523_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HDF2475505.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPDRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYESKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            3185..4369
                     /locus_tag="PC523_001982"
     CDS             3185..4369
                     /locus_tag="PC523_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2475506.1"
                     /translation="MEIRYAKEEDIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNNKVYNTSYVVGVSTLPEGRGTGYMSKVIKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     RIDRAFLNEVNGNVLKDEHYYKNLFKEIQSEDGFLYIHEGNEKDGYIVYFINEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPIIDKIRFILDNPKDSEIKIKPFMMG
                     RIINVKKFIEDVDIENDINSSFNLLIEDKFINENNGLFKISIQNKKVSVEQLYKKDEE
                     KPQEDFDVKLDINTLTQLAFSYIDVNEAIFLNDIKDVSEETLEILNCIFSKKNNYINE
                     YI"
     gene            4484..5785
                     /locus_tag="PC523_001983"
     CDS             4484..5785
                     /locus_tag="PC523_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HDF2475507.1"
                     /translation="MGVIIIQIRRPLLIILVFVIAISFIITNNTKNNEEINDKIITIQ
                     GIVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKFYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLSEEQKEIFTRTGTSHVISISGLHTGILCIIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIIYLSIASLIVFKVSVVIAKIISFFNSTLIESIFFLLEKISNLSFAYINIEKPKF
                     YIVIIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKF"
     gene            5739..6782
                     /gene="holA"
                     /locus_tag="PC523_001984"
     CDS             5739..6782
                     /gene="holA"
                     /locus_tag="PC523_001984"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDF2475508.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGSNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(6972..7238)
                     /gene="rpsT"
                     /locus_tag="PC523_001985"
     CDS             complement(6972..7238)
                     /gene="rpsT"
                     /locus_tag="PC523_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDF2475509.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
     gene            7560..8189
                     /locus_tag="PC523_001986"
     CDS             7560..8189
                     /locus_tag="PC523_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2475510.1"
                     /translation="MNLIKNYKNTEDTADKLISYIDENCSGHLFALCGVSLGGQIVME
                     LLSRKPNITKKAIIDGSICYPRPLMARFCITGIRFFGGLLFSKKACRFQIAMMPKLLP
                     ENMQYPDEIKAYYMTDMPHVRKETFYNMYRTYMMNYILKENVKKTTAQVMYWYGEKEM
                     KCVKKSAKMFQSYVPSCEIYEAKGYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            8531..9484
                     /locus_tag="PC523_001987"
     CDS             8531..9484
                     /locus_tag="PC523_001987"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HDF2475511.1"
                     /translation="MINVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDEQGSEIMNKDIGKYITLESNLMKFDDDESREEMISYLKDELVDIFGQDENKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            9502..10521
                     /locus_tag="PC523_001988"
     CDS             9502..10521
                     /locus_tag="PC523_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HDF2475512.1"
                     /translation="MFKKCIKVVTLTFILACILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTEDKKNNKNKETSKENKEESKEEKTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNATSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            10552..10803
                     /locus_tag="PC523_001989"
     CDS             10552..10803
                     /locus_tag="PC523_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475513.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRIMFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPNIKFDITKFDFDIPNFDIHEIIRKFNK"
     gene            11085..12890
                     /gene="lepA"
                     /locus_tag="PC523_001990"
     CDS             11085..12890
                     /gene="lepA"
                     /locus_tag="PC523_001990"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDF2475514.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDERRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            13039..14376
                     /locus_tag="PC523_001991"
     CDS             13039..14376
                     /locus_tag="PC523_001991"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDF2475515.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMVEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSETGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFNIDEGTEQSIHKKSDLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGHILNNMLEIVINNPELNEKEILKEKALNIYTF"
     gene            14609..15922
                     /locus_tag="PC523_001992"
     CDS             14609..15922
                     /locus_tag="PC523_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2475516.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIIFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITMGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKALLKVNSGGSPVNSI
                     LLITALACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLYRPYRVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            16094..17269
                     /locus_tag="PC523_001993"
     CDS             16094..17269
                     /locus_tag="PC523_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDF2475517.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDLKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFMSIKEMFYIDDN
                     AEITIECNPGTLTLEKLKTMKDIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENIDIEMYEYTINYLMSKGYNQYEISNYSKEGFNCKHNILYWECKPYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFKDKYSKQIEMLSARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            17372..18391
                     /gene="hrcA"
                     /locus_tag="PC523_001994"
     CDS             17372..18391
                     /gene="hrcA"
                     /locus_tag="PC523_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HDF2475518.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLVNQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            18421..19041
                     /gene="grpE"
                     /locus_tag="PC523_001995"
     CDS             18421..19041
                     /gene="grpE"
                     /locus_tag="PC523_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDF2475519.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSNEKT
                     DEKEVDDENVTDINSKLAEKKLQNELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGIEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            19133..20980
                     /gene="dnaK"
                     /locus_tag="PC523_001996"
     CDS             19133..20980
                     /gene="dnaK"
                     /locus_tag="PC523_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDF2475520.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSSEKEEIEKAIADLKTVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            21191..22345
                     /gene="dnaJ"
                     /locus_tag="PC523_001997"
     CDS             21191..22345
                     /gene="dnaJ"
                     /locus_tag="PC523_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDF2475521.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRTRRRGPQRGADIRQSVTIYFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSTCNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            23320..24255
                     /locus_tag="PC523_001998"
     CDS             23320..24255
                     /locus_tag="PC523_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2475522.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            24288..25661
                     /locus_tag="PC523_001999"
     CDS             24288..25661
                     /locus_tag="PC523_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2475523.1"
                     /translation="MSKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMYLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGRWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(25849..26616)
                     /locus_tag="PC523_002000"
     CDS             complement(25849..26616)
                     /locus_tag="PC523_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2475524.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNSIYVIDF
                     LDKNNLDNLKGNFYLIYQPHIEFEYLKNIFYNLFEDINIIQHNSFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGKLDSKADFDFRLQLCSDLLPHIIKDNAENKFLNLFDLI
                     RYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGLKFINNDLNISKTSTSMFLHRNTL
                     VYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
     gene            complement(26646..27755)
                     /gene="ugpC"
                     /locus_tag="PC523_002001"
     CDS             complement(26646..27755)
                     /gene="ugpC"
                     /locus_tag="PC523_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDF2475525.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYDNMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVTLIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRIHIFDKETTLAIR"
BASE COUNT        10922 a         2991 c         4732 g         9183 t
ORIGIN      
        1 ttattttata tataataaaa aaggtaaatt ttaaccatgt atagataaaa cctatttaaa
       61 ttataattta ctatcagata aatctataaa aattgttgca aagagtcaaa atatatgata
      121 ttatagattt cgttgagaag gatttcatta tgggagtgga tgggtgccgg tgtgccctct
      181 agtcttcaaa actagtatga ggggttaaga gcctcttagg tgggttcgat tcccacgcat
      241 tcccgccaat atgtaaaagg tatcttttaa ttaaaagata cctttttaaa tttattttaa
      301 gatagtttat ttttaataat atataatatt ttgtattata taatgtaaat tttctaatac
      361 tattatatat ttagataaaa atctgggagt gtggaaagat ggaaaataat tttacgacga
      421 gaactagttt tctagttgga gatgatggaa tagaaaaact taataattca aacataatag
      481 tatttggagt tggaggagtt ggtagtttta ctgttgaagc tttagcaaga gctggagtag
      541 gtaatattac cattgtggat tttgacgatg tagatataac aaatataaat agacaaatac
      601 cagcactaca ttcaactgta ggaaggtata aagttgatgt tatggaagaa agaatattag
      661 atataaatcc taatatcaat ataaaaaaaa taagaagtct ttacaatcaa gatactagtg
      721 atgaaatact gactgaaaga tatgattatg ttgtggatgc gatagacatg gtaagttcca
      781 aaatacatct aattgaaact tgtgagaaaa aaggattaaa aataataagt tctatgggta
      841 tgggaaataa attagaccca accaaaatag tcgttacaga tatacacaaa acaagcactt
      901 gtcctctagc taaagttatg aggaaagaac ttagggatag aggtatcaaa aagttaaaag
      961 tagtatattc tactgaacaa ccaatagaac ttaagaagaa agtaatgaat ggtagaaagg
     1021 taacacctgg tagtgtatca ttcgtaccat ctgtaggagg tttaataatc gcatctgtaa
     1081 taataaatga gctattagga caataagttt taaatatttt aatttataaa ttttatatat
     1141 aaacttgaag gctagaagaa ttaatataaa atagttttat attaattctt ttagcctaat
     1201 tattttacat taccttattt tatgtagttg tagttttatt tttttgatta tgtatctact
     1261 tcataattat attttgtaaa tataatttct ccatcactat aaaaaacaat atctcctatt
     1321 ctgtctgttc tataaattga tacatggctt tgttttagat tatcaataac ctctttatgt
     1381 ggatgtccat attgattctt atatccacat gatattgcag ctatgtctgg attaattttt
     1441 tttataaatt ctaaagatga tgaagaatta cttccatggt gacctatttt tataaaatta
     1501 atattgttta attcaaaaga acgtaaaatt tctttttcat tttcttcttc tgcatcaccc
     1561 ataaataaaa aagagttatt gttaaaagtt aatttgaaaa ctatagaatt taaattagat
     1621 tcttcttgta tatatgatgg acttaataca tatatattta tattgttatc tatgtttaaa
     1681 acatcatctt tgtagagata ctgaatagct aagtttttat ccgtacaaga atttattaga
     1741 ttttgataag attcactatt agttgattgt tcaggcatat atatggagtt tacattaaat
     1801 ttttttataa cattatcaag acttcctata tggtctgaat ctggatgtgt tgctattata
     1861 atatcaatag tttttattct tttttgtcta agataactat taattatatt tttactatat
     1921 tcatcacctc catcaattaa tatattctta tttgtgggcg tttgaactaa aatactatca
     1981 ccttgtccaa catcaatcat atgtatagat agtagagatt ttttgtcaca gccgattaaa
     2041 agtgatatta taataagtat tgataaaaaa attttatttc tcaataaaaa atcactccat
     2101 tcattacatg tttatataaa tttatgatgg gtaatataag atatatatgg cttattatat
     2161 tttatatttt aaacaatata aatgaaatta atactataat aaaattggag ttttttatat
     2221 atacaaaata aaatttttaa aattaagcaa taaaaaggca ggaggatttt atgtttttca
     2281 aagatattga actaaattca aaaaaagagt tagacccata ttttgattta gtagattatg
     2341 aagcttgtga gtactgtttt agtactttat atatgtggca acatgtttat aagacaggat
     2401 attatatagg tgaagatttt gccgttttag ttggggaata tgagggagat agcttctcaa
     2461 tattaccact tgctaagaaa gataaacttc cagaagttgt agattttgtc cttgaatatt
     2521 tttctaaaaa taataaaaaa atatacttaa gaggtattac tactgaagtt gtagaatttt
     2581 taaaagaaaa atatccagat agatttgagt atatagaaga aagagattta tttgactata
     2641 tttatgatgc agaaagttta agaactctag ctggtaaaaa gaatcaaaag aagagaaacc
     2701 atataaacta tttcttaaaa gagtatgcag gtagatatga atctaaatta ttagataaag
     2761 aaaactttga tgagtgtcta gtattaatga aggaatggga aagtaataag gaagaaaata
     2821 atgaatttga tgaaagtatg gatgatgaat taataggtat caaaaagata tttaaccatt
     2881 atgacatatt aaaagataag gtaaaagtat ttggtgttta tgtggatgga aaattagaag
     2941 cttttagtat aggagaactt ttaaatccaa atatggcatt gattcatata gaaaaagcta
     3001 atccagatat aagaggtctg tatccattta taaatcaaca gtttttggta agtgaattta
     3061 aggatgttga gtttgtaaat agagaagaag atttaggtat tgaaggatta agaaaagcta
     3121 aactttctta tcatccttgt agatttgttg aaaagtacag tgttagggag gcttagtaga
     3181 ctatatggag attaggtatg ctaaagaaga agatatagaa agtataaaag agatttggag
     3241 ttattgcttt aatgatactg agagttttat gaaatattat tttaatgata aatataaatc
     3301 tgaaaatact gtagtagctt tagatgaagg aaaaattatt tcctctcttc agctaaatca
     3361 gtataagtta ctattaaata acaaggtata caatacatca tatgtagtag gcgtttctac
     3421 acttccagaa ggaagaggaa ctggatatat gagtaaagta attaaattta ctttaaacga
     3481 attgtataaa aaaggacaat tagtttctat attgatgcct attgactata ggctttatag
     3541 aagatttgga tatgagcatt gttatgacca gattgaatat actattaaca cagatgattt
     3601 aaaaaatttt aagtcaagtg gaaaaatgat aaagtctaat ttatcacaaa ttgatgattt
     3661 gatacgaata gatagagctt ttttaaatga ggttaatggt aatgtgttaa aagatgaaca
     3721 ttattataaa aacttattta aagagattca aagtgaagat ggttttttgt acattcatga
     3781 aggaaatgaa aaagatggat atatagttta ttttattaat gaagataaaa tgtttgtcag
     3841 agagttattt tataaaaata tagatgccct taaatcaatg cttaaattta tatacaatca
     3901 caatactcaa tgtaagatag ttactatatc tactcctata atagataaga taagatttat
     3961 attagacaac cctaaggata gtgagataaa aataaaacct tttatgatgg gaagaattat
     4021 aaatgtaaag aagtttattg aagatgtaga tattgagaat gatattaata gctcatttaa
     4081 tttattaata gaagacaagt ttataaatga aaataatggt ttatttaaaa tttctataca
     4141 aaataagaag gtatctgtag agcaattata taaaaaagat gaagagaagc ctcaagaaga
     4201 ttttgatgtt aaattagata taaatacctt aacacagtta gcattttctt atatagatgt
     4261 aaatgaagct attttcttaa atgatataaa agatgtttca gaagaaacac tagaaatact
     4321 taattgtatt ttttctaaaa aaaataacta tataaatgaa tatatataaa tagagtacgt
     4381 taaaagatta taaaaatctt ttgacgtatt tttttgttta tataataatt taccttgttt
     4441 aaaaattttc tatttgaaaa taatctaaat aatatgcttt tatatgggag tgattataat
     4501 tcaaataaga agacctttat taattatatt agtgtttgtg atagctatat catttatcat
     4561 tacaaataat acaaaaaaca acgaagaaat taatgataag ataataacta tacaaggaat
     4621 tgtcaaggga aaaattgaaa agaaaagata caatcagtat aaggtaggag tttttttaat
     4681 aaatgattat acaaaaaata aaaatttgaa gattggtcag aaagttaata ttacaggaaa
     4741 atttaaaagt ttagataaaa tgaagtatga cgattttgat tatggaaggt atataaaaag
     4801 cactggatat aaaggtatag tatatataaa taactataaa attattgata aaaataaatt
     4861 ttattcttta ataggagaaa taaagtttta tattagcaag acttataggt acttatataa
     4921 gaaaaactcg gattttataa attctatact attagcagaa gttgaaaatt tgagtgaaga
     4981 acaaaaagaa atttttacca gaacaggaac tagccatgta atatctatat caggtcttca
     5041 tacaggaata ttatgtataa ttatatcctt tctattaaga ggtataaata aattgtataa
     5101 attattaata ctgtcgattt ttataacttt atattgtata atggtaggtg cgtctccatc
     5161 cataattagg tctatagcat ttgttatgat tttttattta tcaatattta ttgatagaaa
     5221 gaaagatgga atatcggcat tgtctctaat tgggataatt ttaattatga ataaccccta
     5281 tgttatatat aatgtaagtt ttcaattgtc ttttttagct acactttcta tattaatatt
     5341 ctataataaa ataaatagta ttataaaatt aagtatggta tcattgacaa tatcatcaaa
     5401 tatattaact ttacctataa tttattatac atttaaaggt ataccattac tttcaattat
     5461 aggaaattta attatagtac catttgtggg agtaataatt tatttaagta ttgcaagttt
     5521 aatcgtattt aaagtaagtg tagtaatagc taaaattata tcctttttta atagtacatt
     5581 gatagaaagc atattttttt tactagaaaa aatcagcaat ttaagttttg catacattaa
     5641 tatagaaaaa cctaaatttt atattgttat catttattac ataggagtgt ttttttacat
     5701 attttacata gaaggaaaag agataaagga gcaagagaat gaattacaag gatattataa
     5761 ggaatgtaaa agagaaaaat tttgaaaaaa tgtatctttt ttatggaaga gagtattatt
     5821 taattgagaa tgcaataaaa gcatttaaag atagtctaaa cgagggaatg cttgatttta
     5881 atttagatat aatagatgga aaggaaatag tcttaaatca gcttataagc tctatagaaa
     5941 cacttccatt tatggatgat agaaaaatag taataataaa agactttgaa ctattaaaag
     6001 gaaagaaaaa gaattttaca gatagtgatg agaaatactt aatagagcat ttagataata
     6061 ttccagacac tacaactata gtttttgttg tatatggtga tgttgataaa agaaagtctc
     6121 tagttaagaa aataggaagc aatggaatcg tttttgattg tgacaagctt tctgatatgg
     6181 atttatttaa atggataaaa aaatcttttg cattaaatga tgttataata gataactctc
     6241 aaataatgta ctttatcgaa caagaggggt atagagataa gagcagtgaa aagacattat
     6301 ctgatttaga aaatgaaata aataaaataa gttcatttgt tggtaaaggg aataatgtaa
     6361 ccaatgatgt aattgataaa ttatctcaaa aaaaagttga gaatgatata ttcaaattaa
     6421 tcgattacat tggagagcaa aatgcttcta atgcaatgaa aatattaaat gatatgatac
     6481 aagaaggcga atctgtatta ggtatatttt ctatgatagc aagacaattt aaaataatta
     6541 tgcaagtaag acagttacaa cttgatggat attcaacaaa acttatagct gataaattaa
     6601 aaatgcatca atttgttgta ggaaaagcat taaagcaaac taaaaatttt tctgatgata
     6661 taattgtgga aatattaaac tatatactag aaagcgatta taaaataaag acaggcctta
     6721 taagagatac cttagcagtt gaaatgttgg ttagtagata ctgtaaaagg gaagcaatat
     6781 aaaaaatcta tatattaata aaaaataaat agcaagatat gttatagttt agagtagatg
     6841 taagctaaat taagtttaag tatatacaat caactataat atgtctaact atttattttt
     6901 tattgtataa atttacaaaa taaaaaaccc ctacataggg gttattatat ataagaaata
     6961 taaactggta attaagcgtt cataccgttt aatttttgag ctaatttagc tacttttcta
     7021 gctgcagcat tcttatgtat agttccttta gaagctactt ggtatattct tttttgagcg
     7081 tattggaact tagcaacagc atcctctctg tttccagctg caacagcatc ttcgaatctt
     7141 cttattgcag tttttatttg agattttcta actctattta aagcagtttt cttttcgata
     7201 acacttattc ttttcttagc tgattttata tttgccaccg tgttcacctc cctgtatttt
     7261 tttatcatat tcatcgtcaa catattagat tttaacagaa ataaatctaa aaatcaagga
     7321 ttattattta tatattttaa taatgttttt tattttgatt ttttagactg tagattgtgt
     7381 ataaataatt tgcaatatct tgtttttagg ttggtatatt ttgtgtttta aatattttga
     7441 catatataaa ttaatatttt atttttgtaa caaatatttt gagataatac aaattattaa
     7501 catacatttt gatagtattt ccaataatta taagataata caagaaatta ctcatttttg
     7561 tgaatttaat aaaaaattat aaaaatacag aggatacagc agataagttg atatcatata
     7621 tagatgaaaa ttgcagtggt catcttttcg ctttgtgtgg ggtatcactt ggtggtcaaa
     7681 ttgtcatgga acttttatct cgaaaaccta atattactaa aaaagcaatt attgatggta
     7741 gtatttgcta cccaagacct cttatggcaa ggttttgtat tactggtatt cgattttttg
     7801 gaggactttt atttagtaag aaggcttgtc gctttcaaat tgctatgatg ccaaaattgt
     7861 tacctgaaaa tatgcaatat ccagatgaaa taaaagcata ttatatgaca gatatgccac
     7921 atgtaagaaa agaaacattc tataatatgt atcgtactta catgatgaac tacatactta
     7981 aagaaaatgt taaaaaaacc acagcacaag taatgtactg gtatggagaa aaagaaatga
     8041 agtgcgttaa aaaatctgca aaaatgtttc agtcttatgt accctcttgt gagatttatg
     8101 aagctaaggg ctataatcat gggtatttat cgctttacct tccaaatgaa tggttggaaa
     8161 ttgcggaacc attttttcaa aaggaataaa ggtgttaggt gattattgta taattattgc
     8221 tgttttttta tatagtaaat aaattaggta tgatataaaa aaattatttt ggtcaatata
     8281 ttagtgaaag cttaatagga aaattataaa tagattaaag gtagggccta tataaatagt
     8341 ttctaccttt ttctgtttgt aaataaatga tagataagaa agtttactga caaatcactg
     8401 accaaaccca attttcttac attgaaagcc ttaattttac ttgttttaaa taaatttaca
     8461 atttcttgtt ataaaatact aaattttgtt aacaattata attagcattt aatttatact
     8521 ggaggtttat atgattaatg taagaacaga tttagcttta gaagctagtg aaatgtgtga
     8581 aaagtctcaa gaaggaagta gcattcctgg tgtaaaaata gaaactaaag aattagaaaa
     8641 ttgtattgta actaaggttg aagtaataga cgaacaaggt tcagaaataa tgaataaaga
     8701 tataggaaaa tatataactc tggaaagtaa tcttatgaag tttgatgatg atgaatcaag
     8761 agaagaaatg ataagttatc taaaagatga actggtagac atatttggac aagacgaaaa
     8821 taaaaaaacg ttagtaatcg gtcttggaaa tagaaatatt acttctgatg cactaggacc
     8881 caaaagtgta tctaaaacac ttgtaactag acatcttttt aaaaattata ataaagatta
     8941 tgatgatgat tttactgagg tatcagcttt aagcccagga gttatgggag ttactggaat
     9001 agagactagt gaaatagtaa agtcattggt tgaaaaagta aaaccagata gagtagtagc
     9061 aatagatgca ttggcttcga gaaaaatgga aagagtaaac tctactatac agatatcaac
     9121 agcaggaata tcgcctggtg gaggtgttgg caatacaaga aaatctctaa ctaaagaaac
     9181 tttaggtgtg gatgttattg caataggagt gccaacagtg gttgatgcag caactcttac
     9241 aatagatgtt ttggacatgg caatagacaa tttaatagct cagtcagaag aaacagaaag
     9301 tttttacgaa atgttgaaaa aacttaaaga agaagaaaag tatcatctaa taaaagattc
     9361 attagaccca tatgacaaga acttgatagt aacaccgaag gatatagatg atacaatcga
     9421 aaatctatct ataataataa gtgaaggtct aaataggtct ttacatccag gtagattggt
     9481 ataaaataag gaggagcaat tatgtttaaa aaatgcatta aagtagtaac cttgactttt
     9541 atattagcat gcattttacc aggaaaatca ttagctttga atcaggatga ttttttaaag
     9601 tttttagtaa attcatctta tccagaagct aaagtcgaag gaaatgatac agaagataaa
     9661 aagaataata aaaataaaga aacaagcaaa gaaaataagg aagaaagtaa agaagaaaaa
     9721 actaaatcta aagatgcaag taaagttgat aataaaaaag agagtgaaaa agaatatata
     9781 aaactttatg ttggtaaaga aaatgttcca gatattgagt caaaaaattc agacacaact
     9841 gaaactaacg caacatctag ttcagattat aaggatgacc taagagtaac taaagaaaat
     9901 ccaagaattt tgatatatca tactcatgga tgtgaaactt attcaaattc accagatgga
     9961 aactaccatt caagagataa gaaaaattca gtaatggaag taggaagtgc attaactagt
    10021 gctttagata gcaagggatg gggtgtagtt catacaacta agtaccatga ctatccatct
    10081 tataataact cttatgcaag tagcctaaaa acaatacaaa gtatattacc taaatataat
    10141 tcagtggaca tagcgataga cttacataga gatgctaggg atttaactaa tccagccact
    10201 aaggaaaaag accatttaaa atatacaact atgataaatg gagaaagggt atcaaaattc
    10261 ttctttgttg taggaggtaa aaatacaaat agaaaacaac ttagagcttt agcagaagac
    10321 ataactgctt ttgctgagaa gaagtatcct ggtttggtat ctccaatagt agaaaaagac
    10381 tatgctagat tcaatcagtt tgcagttaag aatcatatgt tagtagaaat agggaataac
    10441 gcaacaagcg tagaagaatc taaggctact acaaagtatc tagctgaaat tttggatgaa
    10501 tattttaaac aaaaaaatta gagttttaag gtataattag gagcatgtta aatgagccga
    10561 ttagaaaaaa atatagaaag taaaagtaaa ttaagaaaat acaagtatat tatgagaatt
    10621 atgtttttga ttactatgat tatatgcaca tcagtttgtg tatttatagt agatagtaat
    10681 gcaaaaagta tgctaggaat gaaacaatat gattttccca atattaaatt tgatataaca
    10741 aaatttgatt ttgatatacc gaactttgat atacatgaaa tcataagaaa atttaataag
    10801 tagtaataaa tgaataaggt ttattgatga aaataagcct tattttagtt atatatcttt
    10861 aatgtataaa ataaaaattc actaactgta ttttatagga tttactttaa gtttatgata
    10921 ttatataata tttagtgtta gacttgataa tccacatata atgtggataa ttttgtttag
    10981 tgccaattag tttatattca aaaattttaa atttggtata taaagagttt ttagataatg
    11041 taaaataata actataagaa taatcaagag gagggaactt taaggtggac aataaacaaa
    11101 gtagaactag aaattttagt ataattgcac atatagacca tggaaagtct accttagctg
    11161 atagattaat acaacaaact gggcttgtta gtgaaagaga catgaaaagt caattacttg
    11221 acaatatgga ccttgaaaga gaaagaggaa tcactataaa gcttcaaaac ataagattaa
    11281 tgtataaagc gaaggatgga aacgaatatt atttaaatct tatagataca cctggacatg
    11341 tagactttaa ctatgaggta tcaagaagtt tggctgcatg tgagggagct ttattagtag
    11401 ttgatgcagc tcaaggtgtt gaggcacaaa ctttagctaa tgtttatctt gctatagacc
    11461 aagatttaga aatattacca ataataaata aaatagattt accaagtgca agaccagagg
    11521 aagtaaaaaa tgaaatagaa gatttaatag ggcttgactc aagtgaagca cctcttatct
    11581 cagctaagac tggtttaaac atagaggatg ttttagaaga cattgtaaaa aatgttcctc
    11641 cacctaaagg agataatgaa gctccattaa aggcattaat attcgattct tactatgatg
    11701 cttacaaagg tgttgtagcc tatgttagag tatttgaagg aactgttaaa aagggtatga
    11761 caataaaaat gatgaatacc aataaaaagt ttgaggtaac tgaagttggt gttatggctc
    11821 caggtcaaac tgaacttagt gaattatcag caggtgatgt tggttatatt gcagctagta
    11881 taaaagatat aagaagttgt cgtgttggtg atacaataac agattcaaat aatcctacag
    11941 aagaaccttt accaggatac aaaaaagcta ctccaatggt atattgtggt atatatccag
    12001 gagaaggaga aaaatatgaa aatgttagag atgctcttga aaagcttcaa gtaaatgatg
    12061 ctgcacttga atatgaagct gagacttcag ccgcactagg atttggtttt agatgtggtt
    12121 tcttaggtct tttacatatg gagataatgc aagaaagact tgagagagaa tttaatcttg
    12181 atataattac tacagcacca tctgttatat atagagttac aaaaatggat ggagaagtcg
    12241 taatgataca aaatcctgca aacttgccag aaccatctga gataaagatg atagaagagc
    12301 ctatagtaaa aggagatata atagttccta aagactatgt aggtgttgtt atggaacttt
    12361 gtcaggaaag acgtggaaat atgttgaaca tggagtatat agatgagaga agagtaatgc
    12421 ttcactatga ccttcctctc aatgaagttg tttacgattt ctttgatgcc ttaaaatcaa
    12481 gaactagagg ttatggttct cttgattatg aggttaaagg ttatgtagca tctacacttg
    12541 taaaactcga tatattaatt aataaagaac aagtggatgc acttagtttt attgtccatg
    12601 aaacacgtgc tttccctaga ggaaaggcaa tgtgtgagaa attaaaaggg gaaattccaa
    12661 gacatcagtt tgctatacca atacaagctg ctgttggtaa taaggttatt gcaagagaaa
    12721 caataagtgc tctaagaaaa gatgtccttg ctaaatgtta tggtggggat atatctcgta
    12781 agaagaaact tcttgaaaaa caaaaagaag gtaagaagcg tatgagacaa attggttctg
    12841 ttgaagttcc tcaaaaagca tttatgtctg tgcttaaatt agatgaataa atccttataa
    12901 atttagataa agatatttaa aagctagagt gattttagaa ttatttctaa attagtctag
    12961 cttttttatg gtatatttta agtaaactca taaagaatat aagtaagaat attaaagaag
    13021 aataaaaagg tgatatttat gattaatata gaaattccta aaaaagtaga ttatataatt
    13081 aaagaattag aaaagaatgg ttatgaagct tatatagttg gaggatgtgt tagagattgt
    13141 ttattagaaa gagttccaaa cgattgggat ataacaacta gtgctagacc agaagtggta
    13201 gtagaattat ttgaaaaaac aattccaact ggtatacaac atggaactgt aacagttatg
    13261 gttgaacatg aaccatttga agtgacaact tatagaattg atggaaatta tagtgatgga
    13321 agacatccag attcaataga atttacaaat aacatagtta aggacttatc aagaagagat
    13381 tttaccatca attcaattgc atacaattca gaaacaggat tagtcgaccc atttaatgga
    13441 tatgaagata tacaaaataa atatatacgt tgtgttggaa atccagtaga taggtttgaa
    13501 gaagatgcgc ttagaatgct tagagcagtt agattttcag cacaacttaa ttttaatata
    13561 gatgaaggta ctgagcaaag tattcataaa aaatcagatt tgataaaaaa tgtttctatt
    13621 gaaagaatcc agacagagtt taataaaatc ttagtatctg attcatctaa attaaaccta
    13681 ttaaaatcaa caggtttgtt gaaatttata attcctgaaa tatgtgaatt ggaagatgtc
    13741 actcaacaca atccatatca tatctatgat gtacaaaaac atactttaat agctacagaa
    13801 gtaattgaag atgaactgta tttaaaactt acaatgttgt ttcatgattt aggtaaaaaa
    13861 gtaactaaaa caactgataa aaatggtgtt gaccattttt atactcatag tagagaatct
    13921 gtaaagattg caaagaagat tttgaaaaga cttaagtatg ataattatac tattaataaa
    13981 gttttgatat tgatacaata tcatgattat agaatagaac ctaaaaggaa gataattaaa
    14041 aagttattaa ataaacttga agatgttgaa ttatttgaag atttaataaa agttaattgg
    14101 gctgacacac ttgccaaaaa tccaaaatat gcaaaacaaa agatattaaa tcttattgag
    14161 tgtgaaaaag aatttaaaca tataataaat caaaaggagt gctttaatct taaagattta
    14221 gctattaatg gaaaagattt gatatctata ggtataaaac caggtaaaga cataggacat
    14281 attttaaata atatgcttga aatagttata aataatccag agctaaatga aaaagaaata
    14341 ctaaaagaaa aggcattaaa tatatataca ttttagattc aaatataaaa aatttaatag
    14401 atttaagaaa tatagaagaa aatatgtatt tagataaaat aaataatttt tagagaatat
    14461 tttagagatt acgattgtat gagttttaaa tgcataggga tttactctag gattatagac
    14521 taatataaaa aaaaactttg ggaatactaa aaccataaat ttataaaatt aatattttat
    14581 atactgtaaa acagtgagga ggaacagtat gcatactcaa ctacaaaaga ctattggtct
    14641 ttcagcagct ctttctactg ttgtaggtat ggttataggt tcgggagttt tttttaagcc
    14701 acaagcaata tacactacaa ctaatggagc tccagggcta ggtattatag catggctttt
    14761 aggtggtttt ataacaataa ctgctgggct tacagctaca gaaatttcag ctgctatacc
    14821 gaaaactggt ggtatgatga tttatatcga agaaatatat ggagaaaagt taggtttttt
    14881 aactggatgg atgcaaactg tactattttt cccaggtaca tcagcagcac taggagtaat
    14941 atttgctcaa caagcatctg aacttttagg aatgagccca aataatatgg caaatgtact
    15001 tcctatagcg attggagtta tactattttt agcattatta aatataatag gttcatcttt
    15061 aggaggaaaa gttcagacag tagctaccat tggaaagatg atacctttaa ttttaataat
    15121 aatctttgga tttattaaag gtcaaagtag tgaagtgtta aatccatttg taggagatgg
    15181 tgtaaatgca tcaaatgcac ttggacaagt attaatagct actttgtttg catatgatgg
    15241 ttggataaat gttggagcta tatcaggaga aatgaagtct ccagagaaag acttaccaag
    15301 agcaatagtt ggtggtttat cattagttat ggcagtttat attataataa atatagctta
    15361 tctttgggta gtaccagcaa gtgaattagc aacagttact tcaccagcta cattagtagc
    15421 aacaagattg tttggaaata taggtggaaa agtaataact atgggtatat taatatcagt
    15481 atttggcaca ttgaatggat atttattaac aggttcaagg ataccttata cattagctga
    15541 aatgggaact ttaccagcat ctaaagctct tttaaaagta aattctggag gttctcctgt
    15601 aaattcaata ctacttataa ctgcgttagc atgtgtatat gcattatctg gtcaatttaa
    15661 tcttttgaca gatttaacta tattttcaat atgggtattt tatgtattaa cttttatagg
    15721 tgtcatgagg cttagaagac aaaagccaga tttatacaga ccatatagag tccctttata
    15781 tccaataata ccgattatag ctatattagg cgggctgttt gtaataataa atcaaatatt
    15841 aacttcaaca gttatatctc taggcggaat atttataaca ctattaggtt taccagttta
    15901 ttattatatg aaaaaaagat aactacttaa tttaagttag atgaatttgg gtaaaagtaa
    15961 aaattaactt ttatccaaat tttttatact attacacaat ataagtatta tatattactt
    16021 gatgtgctat tttgtttgat ttataattta gtaattgatg ttttaagata atttaagaaa
    16081 tgaggaataa aaaatgctag gactttatgt acatattcca ttttgtgtga aaaaatgtaa
    16141 gtattgtgac tttaattctt ataaaatgga catagattta aaaaaaagat atatagaaga
    16201 tttaaaaata gaaatggaat tatatagtaa taaattatat aaagataata aatataaaaa
    16261 taaagaatgt tgtagtctaa ataaaaatga caaaataacg agtatatttg tagggggagg
    16321 tacacctagt atactaacat ctgatgaaat aagagaagta tttatgagta taaaagaaat
    16381 gttttacata gatgataatg cagaaataac tatagagtgt aatcctggta cattaacatt
    16441 agaaaaatta aaaactatga aagatattgg tataaataga cttagtatag ggctacaagc
    16501 tattcaagaa aagcatttga attttatagg aagaatacat acttatgaag aatttgagaa
    16561 aaactataaa gatgcattaa gtgtaggatt taaaaatatt aatatagatt taatgtacag
    16621 tcttccaaat caaactctat gtgattggaa ggaaactctt gaaaaagttg tacacttaaa
    16681 tccaacacat atatcagctt attctcttat actagaagaa ggcactgaac tatacaatat
    16741 gtatgaaagt aataaatttg aacttataga tgaaaatata gatatagaaa tgtatgaata
    16801 tacaataaac tacctaatgt caaaaggata taatcaatat gaaatatcaa attactcaaa
    16861 agaaggcttt aattgtaagc ataatatttt atattgggaa tgtaaaccgt atatagggat
    16921 tggagcagga gcttctggct atattaatga gaacagatac aataatgtag aatctttaga
    16981 agactatcat ttaagtttgg taaaaaggga aaaaccaata caagaaaatg aaattctttc
    17041 tgagaaagat atgatagaag aaaagatatt tatggggctt agaatgaata aaggtataaa
    17101 atttgaagat ttcaaaaaaa aatttggaat agattttaaa gataagtata gtaagcaaat
    17161 agaaatgcta tcagctagaa agcttataaa tcagagcttt gaaggtatac aattaactca
    17221 aaaaggaaga gaaatctcta atagtgtatt tatagaattt atggaataat tattaaggtg
    17281 ttgacaaaat aaaattataa tggtatataa tatatataat ctttttagca ctcgaactta
    17341 gtgagtgcta acagctagga ggtataataa tatggaatta aatgaaagaa aattaaatat
    17401 cctcaaagct atagttaaag attatataga aacagctgaa gctataggtt ctagaacaat
    17461 atctaaaaga catgatttag gtgttagtgc tgctaccata agaaatgaaa tggctgacct
    17521 tgaagaatta gggtatttaa ttcagcctca tacttctgcg ggcagagttc catctgaaaa
    17581 aggctataag ttatatgtaa actctcttat gagtaaaagc gagttggatg ataatgataa
    17641 gattcttatt gaacaatgta tgaatcataa tataaatcat ataaaagaat tgattcatga
    17701 aacttctaag ctattatctc aactaacaaa ctatacaact gtagcagtta ctaagagttt
    17761 agtaaatcaa agtgttataa agcacataca gttggttgct atgaatgata ataatatttt
    17821 acttatagtt gtaacagata aaggggactt gaaaaaagct aaccttacaa ctaatgtata
    17881 tttagatcaa tctaagttaa atttgatttc tgataatctt acaaggaagc tcttaggtaa
    17941 aagtataaca gatttggatg ataatctaat tgcttttatt aaatacgaaa taagtgagta
    18001 ttctgggctt atagatgagc ttttaaatgc tttgaactca aatatgaaag aagaagattt
    18061 ttctctatca ttaaatggag ctactaacat atttagttat ccagaattta atgatgtatt
    18121 aaaagcaaag tcatttttaa atatgttgga gaaaaaggaa acaatagctg acataattaa
    18181 atcaaaggga atacaaaaag acaatctaaa tataataatt ggtagtgata atgattgtga
    18241 actggcacag gattgtagta tagttacagc aacttataat gtagatagag acttggttgg
    18301 acgaataagc ttcataggtc ctaccagaat ggattatgct agaatttact ctataataaa
    18361 ttatatgagt ttattaatta atagaaagta aagaaatttt aaattgcgag gtgtaaagtc
    18421 ttggataaaa aaaatgagca acaggaagta agagaagaaa atgatacttc tattaaccaa
    18481 gaatcggaaa ctcaagtaga gttggaagag gaagttgtta atgaagaatg tgaaacatca
    18541 aatgaaaaaa ctgatgaaaa agaagttgat gatgaaaatg taacagatat aaattctaag
    18601 ttagcagaaa aaaaattgca gaatgagttg gatgaattaa atgataaata tcaaagactt
    18661 caagccgaat atgcaaatta cagaagaaga actcaacaag agaaagagac aataggtgta
    18721 tttgcaaatg aaaaaataat aactgagttg ataccagtta tagatagtat ggaaagagcc
    18781 ttagatgcat gtgaagataa ggaagatact atgtataagg gtataagcct tgtacataaa
    18841 caattaatag acactttagt aaaatttgga gtagaagaaa tagaagcaga atctaaggaa
    18901 tttgacccta atttacatct agcagttatg caagaaagtg tagatggaat tgaagcaaac
    18961 caaatagtaa tggtacttca aaaaggatat aagctaggaa ctaaagttgt aagaccttca
    19021 atggttaaag tttcttgcta agtataaatt aaaggttaca ttatttaacc taatataaaa
    19081 tattaaaata agtaaaactt aattatataa ttactcgagg aggattttag tcatgggaaa
    19141 aataatagga atagatttag gaacaacaaa ttcatgtgtt gcagtgttag aaggtggaga
    19201 agcacaaata atagcaaata gtgaaggtat gagaactact ccatcagtag tagcatttac
    19261 aaaagatggt gaaagaatag ttggagaacc tgcaaaaaga caagcagtta caaatgcaga
    19321 taaaacaata acttctataa aaactcatat gggaactgat tataaagtaa atatagatgg
    19381 aaaatcatat actccacaag aaatatcagc aataatatta caaaaattaa aatcagatgc
    19441 agaaagttac ttaggacaaa cagtaacaga agcagttata acagttccag cttactttac
    19501 agatgctcaa agacaagcaa ctaaagatgc tggtagaata gcagggcttg atgttaagag
    19561 aataataaat gagccaacag cagcagcact tgcttatggt atggataaat tagaccaaga
    19621 aaagaaaata ttagtatttg acttaggtgg aggaactttt gacgtatcta tacttgaaat
    19681 aggagatgga acttttgaag ttttagctac agctggtaac aatagattag gtggagatga
    19741 ctttgaccaa atagtaatag attatttagc agaggaattt aaaaaagctg aaggtgtaga
    19801 tttaagaaat gataaaatgg ctcttcaaag attaaaagaa gcagcagaaa aggctaagaa
    19861 agaattatca tcaacaatga gttcaaacat aaacttacct ttcataactg ctacagcaga
    19921 aggaccaaaa cacttaaata tagacttatc aagagctaaa tttgaagaat taactagagg
    19981 tttagttgaa aaaactatgg aaccaactaa gacagcttta caagatgcag gactttctac
    20041 aggtgatata gatgatgtat tattagtagg tggttctaca agaataccag cagtacagga
    20101 agctgttaag aagtttatag gaaaagagcc tcacaaaggt ataaacccag atgaatgtgt
    20161 tgcagcaggt gcttctatac aagctggagt tttagcaggg gatgttaaag acttattatt
    20221 actagatgtt actccattat ctcttgggat agaaactatg ggtaatgtaa tgactaagat
    20281 aatagaaaga aatactacaa taccaactaa gaagtctcaa atattctcaa ctgctgctga
    20341 taatcaaaca gctgttgata tacacgttct tcaaggtgaa agaagtatgg cttatgacaa
    20401 tacaacttta ggaagattcc aattaacaga tataccacca gcacaaagag gaatacctca
    20461 aatagaagtt acttttgata tagatgcaaa tggtatagtt aatgtttcag ctaaagattt
    20521 aggtacagga aaagagcaaa aaataactat aacatcaaac actaatttat cagaagcaga
    20581 aatagagcaa aaaataaaag aagcagaaat gaatgcagaa gctgacaaac aaaagaaaga
    20641 aaaaatagaa gcattcaatc aagctgaatc tactatatat caaactgaaa aaactttaaa
    20701 tgaacttgga gataaaataa gttcaagtga aaaagaggag atagaaaaag ctatagcaga
    20761 cttaaaaact gtaaaagata atcaagatgc tacagcagaa gaacttaaga aagctacaga
    20821 tgaagtaatg actaagttcc aaaaagtatc tcaagagatg taccaaaaag cagctcaaga
    20881 gcaacaagca gcacaaggtg cagaacaagc tcaagacaat ggacctaaag atgacaatgt
    20941 agtagatgct gactttaaag aagtggatga agataaataa gctatataat atgtaacact
    21001 aaatgatagt tagagcatat atgatttgtg aaaatcatat agatgttaaa gatatatgga
    21061 caatacacat cataaagaat ataatatatc atagattata taacttaaag tagaatataa
    21121 gaattaatga tagcttgtag tttattattc tacaagctat cgttttgtaa aatgaaggtg
    21181 gtgtcagttt ttgagtacaa aaagggatta ttatgaagtc ttaggtatca gcaaaggtgc
    21241 agaagctcaa gagataaaaa aagcttacag aaaattagca atgaagtacc atccagatag
    21301 aaatcctgga gataaagaag cagaggaaaa atttaaagag ataaatgaag cttatgaagt
    21361 cttatctgat gatacaaaga gaaaaactta tgaccagttt ggacatgatg ggctaaatgg
    21421 tcaaggtggt tttggtggtc aaggaggatt tggcggtcaa ggttttggtg gttttgaaga
    21481 tatgttcgga gatatatttg gtgacatgtt tggaggaagc ttcggaggag gaagaacaag
    21541 aagaagagga cctcaacgtg gtgcagatat aagacaaagt gttactatat attttgaaga
    21601 agctgcattt ggtaaaaaga tgtctataaa agtaaataga agtgaagagt gtgaagaatg
    21661 taatggaact ggagcaaaac caggaacatc aaagaaaact tgttcaactt gtaatggaac
    21721 aggtcaagtt agaactgttc aaagaactcc ttttggtaat atagcaagtt caagaccatg
    21781 tagtacttgt aatgggactg gagaagtgat tgaatctcca tgtagtaaat gccatggaac
    21841 tggaaataca agaaaagtta agactataga agtagatata cctgctggta tagatgatgg
    21901 tcaaatgata aaactttctg gtcaaggtga agtaggagaa aaaggtgctc caagaggaga
    21961 cttatatatt gtagttaatg ttaagtcaca tccactattt actagagatg gaaatgatat
    22021 ttattttgaa atgccaatta catttgttca agcaacttta ggtgatgaga tagaagtacc
    22081 tactttggat ggaaaagtta aatatagtgt accagagggg acacaaactg gaactgtatt
    22141 tagacttaaa gaaaaaggaa tccctagaat tagaggtaat tcaagaggag accaatatgt
    22201 taaggttgta gttgaaattc ctaaaaaatt aaacgataag caaaaagaac tattgagaga
    22261 atttgcgaag gagtgtgggt cgaacgtcca cgaaaagaag aaaaccttcg gacaaaagat
    22321 agaagatatg tttaagaaaa agtagttaaa gtagccaata tataatcata tttttgaaat
    22381 cttagttatt tataagattg tcaaaggaga tattatgatt tttatattgg ctatttttgt
    22441 ataaaaaata aatatgcata aaataaaact gaatataaat aattttctga aaaatacttg
    22501 tcctaaaata tataatatgc tataatataa tcaaataaaa gaaaggggtg attccagtgg
    22561 gaacggatgt ggcactgcca gatgacgaag aaaactttat tgtaatctac aaaataaaat
    22621 tagatgcttt tagtgctaga ttaagtactg gaaaaactca tgagctggga taattattat
    22681 aattacctag acttaaattt ttagtagtaa aatagtaata ttatatagac aaggtatcta
    22741 atttttaaat cttttgtatg aattaaaatt tttataaagg atatatctga ttagaatgta
    22801 attagggaaa cctagttgta aattgctttt agtatataca tgtgtaaaga aatatgtata
    22861 tggtattggt tattgtgcta gatgcatatg taaaggaata tgtatttagt tgagtatatg
    22921 taaagaaata tacttataat ctatgaaaaa attttagtat aaattgctaa aacattgaga
    22981 ttgtttttta tagaaagtta gtttatacca attaaaaatg tgcttaagat ttgtttgatg
    23041 tttaattata aataagattg tagataaaaa aataagtaga gacaaaggtt taaaatactt
    23101 gatttaaata tagtttgttt tgaacacttg ccaaattaac caaattaaaa taattaataa
    23161 aatagacatc ttctcatagt aaaatatact gtgagaggat gtttttgctt tgttcataaa
    23221 cttttatgaa aaatttgcat agtgtcacat tttaatatac tatatatata ataaaatata
    23281 gataaaatta aatgattcaa aagagaaagg tgaaaagaga tgtattatat aggtgtagac
    23341 ataggaggaa caggtataca agcaggagta gttgataact atgggaagat aatttttagg
    23401 tcagagtgta aaacagtaat tgaaaaaggc tttgaaggaa tacttaatga cataaaaata
    23461 atgatttata agttgttgga agataataaa cttactatga gtgacataaa atctattgga
    23521 tttggtgttc caggttttat aaataaagaa ggattagtaa cttgtgtaaa tttaaaatgg
    23581 aataaaaaag cttttaataa agagttaaaa agaagatttc cagatgttga aattcatgga
    23641 gaaaatgatg caacagtagc agcacttgga gaagcaaagt ttggaagtat gaaaggtgct
    23701 aatgttggag tattatatac tttaggaact ggtgtaggtg gaggaatagt aattaatcaa
    23761 aaggtctttt ctggagcaca tggattgggt tcagaaatag gtcatcaaat tataggagaa
    23821 aactatttta attgtaattg tggaaataat ggttgtgtag aaactttttg ttcagcaact
    23881 gcaattataa agtattctca aaagctgata gaagaaggag aaaagagtag gattttggat
    23941 ttggcagagg gcaatttaga aaatgtaaat gcaaagatgg tgtttgatgc atatagagaa
    24001 aatgatttag tggcaattaa agtaataaat agatttaaag aatatttggc aaaaactttt
    24061 gcaaatacaa taaattcttt agacccagaa attatatcca taggaggagg tatttctaaa
    24121 tctagcgata taatacttga tgggatagaa gatttagtaa gaaaatttgt attatacaaa
    24181 acagaagata tagctacaat tacatgtgca actttaggtt cagacgcagg aattatagga
    24241 gcagcttttt tataaagtaa atgaactgct tattggaggc gggatatatg agtaaaacaa
    24301 atgctagaag aacgcttttt ttgatagcca taggttcagg aactatgctg aatcctctta
    24361 attcttctat gatttcactt gctttacata gtatacaaaa tgattttcat attagtttta
    24421 caactgtatc ttggattgtt tcagcatttt acctagctag tgctattgca caaccagttt
    24481 caggtaaaat aggagattta ataggtagga aggtcttatt tttgagtggt ttgatgcttg
    24541 taattttatc agcatccata gtgccacttg ttcaatcatt ctttatattg atttttataa
    24601 gagtaattca ggcaattgga acgagtacac tatatccatc tggagttgct cttatacaaa
    24661 ataatataaa agaaagacag tcttctgcat tagcagttct aaccattttt gcttcaacta
    24721 cagcaggatt aggtccaacg cttggaggat tattgttgga tttaggagga tggcatgcta
    24781 tatttcttgt aaatatacct gttgttttgg tttctatatg cttagggtat tttttattcc
    24841 caaaagaagt aaaagaaaag aaatctataa aagaaactct gaaaaatgtt ttgtcaagat
    24901 tagatattat tggcatactt ctattttcaa ttgcaatgat ttttatatta ttatttttgc
    24961 tatctatgaa ggagtctttc aacttagaac aacttgtatt tggtataata ctgatgtatc
    25021 tttttatttg gcatgaattg agaacaaaat taccatttat agatattaag ctttttgtga
    25081 gtaaccctaa attgtcaaaa gtttacttgc aatttattat attaaatttc tttaattata
    25141 ttttgttttt tggtcttcct agttattttc aagatgcatt acactatagt gcaaaatcga
    25201 ctggattatt tatgctattt atgtctggta taggtatatt tatatctcca ttaactggaa
    25261 gatggataga taaatctgga actagatttc ctcttgttac atcatcaatc tttatgttta
    25321 ttagtgcagt acttctttca gtagttttta ttcatatacc agtaatagga aaaggtatca
    25381 tattatcatt agcaggtatt agttatggag ttggtaatgt agccctgcaa tcctctatgt
    25441 ttgaagaaag tcctcgtgac agtataggga cagcttctgg cctttttcaa actagtcgtt
    25501 atttaggttc tattttagca actgttttac ttggaatggt atttgaagta aatataacat
    25561 cagaacaatt tcagatatta ggttatgtga tggtggtctt aggtacaata agttttttat
    25621 taaattttat actaaagaaa gaattaagaa aagtagaata gtggatttat ggatttaata
    25681 agtctaataa ctgataagtt taaaaacatt gtttgtaaaa tataactagt ttgatattta
    25741 ttaattctaa gctttgaagc atagagtact cgataagtat acaatatttt gaattaaaat
    25801 taagcagaaa tttgaactag tctttcaaat ctctgcttaa tttagtggtt aaattttttt
    25861 atagagaaaa taagatttga cactcagata aaaaatcatt gcattttcaa aattctttaa
    25921 atcaaaccca agtatctcat taatcttttc caatctatat actagcgtgt ttctatgtaa
    25981 aaacatacta gttgatgtct tagaaatatt taaatcatta tttataaatt ttaatcctgt
    26041 atatagaagt tcttcatcaa tagatttaat cttattaaaa tctattaatt tattgagtat
    26101 attgtcctca ttaattaaat ctaaggtttt atatctaata aggtcaaaca aatttaaaaa
    26161 cttattttca gcgttgtctt ttataatatg aggtagtaaa tcagaacata actgtaatct
    26221 aaaatcaaaa tcagctttac tatctaattt accaagataa aaaatataag tactttgata
    26281 agtttcagtt tcaattatat tttgtgtagt tacattaata tctaagttat atttagaatt
    26341 tactataaaa aaactattgt gttgtattat atttatatct tcaaataaat tataaaaaat
    26401 attttttaga tattcaaatt ctatatgagg ttggtaaatt aaataaaagt tgccttttaa
    26461 attatcaaga ttatttttat ctagaaaatc aataacataa atagaattat tgaaagtaac
    26521 taaatggcta ctagcatcta acttttggtt ttcatatgga tagatattta tttttttatt
    26581 aagttgtctt tctaaaaaat cctttaactc ttccataaag tctccttaaa aataagatta
    26641 tattcttatc ttatagccaa agtagtttct ttgtcaaata tgtgtattct actgttatca
    26701 actgaaagtg ttaagtcatc accaacatta attctgtttt tactgtcaaa tctaattgtt
    26761 atagtatttt cgcctagctt taagtgagca taagtttctg ctcccataag ttcttttatt
    26821 tcaattcttg atttaaaaca agtatctgga ttagatttaa taaaagtatc ttctacatga
    26881 atatcttctg gtctgatacc tataacaact tcattattaa tataattgtc ttttattaaa
    26941 tcatagtgtt tttctttatt taatttaagc ttaatatgtt cattttgtgc ataaatttca
    27001 tcattttctt ctattaatgt tacatcaata aaattcatct gtggtgctcc aataaatcct
    27061 gcaacaaata tattagctgg ataatcgtat acatcttgtg gagttgcaat ctgttgaacc
    27121 actccatctt tcattactac gattctatca gccatagtca tagcttcaac ttggtcgtgt
    27181 gttacataga taaatgtagt tccaagctgt tggtgtagct tagttatctc tgttctcata
    27241 gcagttctaa gctttgcatc taagttggat agaggttcat ccattaagaa taccttagga
    27301 tttctaacta ttgcacgtcc taaagcaacc ctttgtcttt gtcctccaga caatgtttta
    27361 ggttttttat ttaaaagagg tagtaaatct aatattttag cagcctcttt aacttttttg
    27421 tctatttcat ctttaggcaa ttttctaagt ttaagtgcaa atgccatgtt atcatagacg
    27481 gataagtgag gatatagagc atagttttga aaaaccatag ctatatctct atcttttggt
    27541 tctatatcat tgacaagttt atctccaatg tatagttctc cttcagagat ttcttcaaga
    27601 ccagcaatca ttctaagtgt agtcgattta ccacaaccag atggtcctac taaaacgata
    27661 aattctttat ctttaatgtc tatgtttata ttttttactg cattaaatcc atttgagtaa
    27721 agttttgata tattttttaa aattacttct gacataatat tctccttgat atttaaattt
    27781 taattatata gtatcaaatt tttttattat attaaatgtg taaaatga
//
LOCUS       DAMJVM010000043        27230 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.044, whole genome shotgun sequence.
ACCESSION   DAMJVM010000043 DAMJVM010000000
VERSION     DAMJVM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27230
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.044"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            <1..745
                     /locus_tag="PC523_002002"
     CDS             <1..745
                     /locus_tag="PC523_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2475526.1"
                     /translation="FKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEHPEVDFVQLQI
                     NYLDWDNESIQSRKCYEVAQKHNKPVIVMEPVKGGTLAKIPEKAEKLLNDYNSDMSIP
                     SWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFIQEEYNIIDEAVGIINESI
                     LVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNYTKTYGKASDC
                     IECKQCEESCPQHIKIIDALKNVAEIFEK"
     gene            781..1194
                     /locus_tag="PC523_002003"
     CDS             781..1194
                     /locus_tag="PC523_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2475527.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIQARKELLIEQRNQLAKRMEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEEVSYE"
     gene            1187..1726
                     /locus_tag="PC523_002004"
     CDS             1187..1726
                     /locus_tag="PC523_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2475528.1"
                     /translation="MSKHVLIVSASPRKGGNSDLLCDQFTQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYKKEASCPQKDDMGEILEKMIEADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVSAADENIADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNI
                     GDIKGSEAMKQAYEMGKMA"
     gene            complement(1916..2401)
                     /locus_tag="PC523_002005"
     CDS             complement(1916..2401)
                     /locus_tag="PC523_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475529.1"
                     /translation="MNKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEKCSKTIKNSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEIDWKNLEKLITKENNIMQES
                     V"
     gene            3253..3630
                     /locus_tag="PC523_002006"
     CDS             3253..3630
                     /locus_tag="PC523_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2475530.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            4030..5838
                     /locus_tag="PC523_002007"
     CDS             4030..5838
                     /locus_tag="PC523_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2475531.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEVSKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHVKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNICKNKDINIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQS"
     gene            6321..7535
                     /gene="dpaL"
                     /locus_tag="PC523_002008"
     CDS             6321..7535
                     /gene="dpaL"
                     /locus_tag="PC523_002008"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDF2475532.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLRDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSDEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGAEASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            7780..8967
                     /locus_tag="PC523_002009"
     CDS             7780..8967
                     /locus_tag="PC523_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HDF2475533.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGINAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKSV
                     YKK"
     gene            8983..10566
                     /locus_tag="PC523_002010"
     CDS             8983..10566
                     /locus_tag="PC523_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HDF2475534.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDDNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDVIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIRDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            10594..11922
                     /gene="ssnA"
                     /locus_tag="PC523_002011"
     CDS             10594..11922
                     /gene="ssnA"
                     /locus_tag="PC523_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDF2475535.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTNEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISSEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            12067..13419
                     /locus_tag="PC523_002012"
     CDS             12067..13419
                     /locus_tag="PC523_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDF2475536.1"
                     /translation="MSQDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLRFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVILNIFFNELGSKKEKNNKVEEAK
                     "
     gene            13609..14718
                     /locus_tag="PC523_002013"
     CDS             13609..14718
                     /locus_tag="PC523_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2475537.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKKTGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMTARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            14997..16388
                     /gene="hydA"
                     /locus_tag="PC523_002014"
     CDS             14997..16388
                     /gene="hydA"
                     /locus_tag="PC523_002014"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HDF2475538.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGATKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     EGLEEWHEKAKDKCYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKAENNLSPKYHQLTRPAILEAE
                     ATSRLMKIAEVANSKVYVVHLSCRESLEEVLKAKQRGVDVIVETCPQYLLLNDGLYDL
                     DGFESAKYVMSPPLRDKENNEILWNAISDNQIDTIGTDHCSFNYRGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGAIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            16382..18943
                     /gene="xdh"
                     /locus_tag="PC523_002015"
     CDS             16382..18943
                     /gene="xdh"
                     /locus_tag="PC523_002015"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HDF2475539.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVREHLVRGNAEEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAIAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     VTQVVCETANLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASSKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVMLDENGRLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVKSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            19095..20441
                     /gene="rpoN"
                     /locus_tag="PC523_002016"
     CDS             19095..20441
                     /gene="rpoN"
                     /locus_tag="PC523_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDF2475540.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            21187..22209
                     /locus_tag="PC523_002017"
     CDS             21187..22209
                     /locus_tag="PC523_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2475541.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSSL"
     gene            22269..23276
                     /gene="gap"
                     /locus_tag="PC523_002018"
     CDS             22269..23276
                     /gene="gap"
                     /locus_tag="PC523_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDF2475542.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            23498..24700
                     /locus_tag="PC523_002019"
     CDS             23498..24700
                     /locus_tag="PC523_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDF2475543.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEEMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEGYMGLDMGPKTIANFVKTIKESKTIVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            24726..25469
                     /locus_tag="PC523_002020"
     CDS             24726..25469
                     /locus_tag="PC523_002020"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDF2475544.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCRVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            25478..27010
                     /locus_tag="PC523_002021"
     CDS             25478..27010
                     /locus_tag="PC523_002021"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDF2475545.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKATSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAVKAVETVDTCVGKLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYENAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT        10936 a         3076 c         5283 g         7935 t
ORIGIN      
        1 ctttaagttt attgaagaaa agaaaaagga aggaaaaata aagaatattg gtttttcttt
       61 tcatgatagt gcagaattat tggataaagt cttaagtgag catccagaag tagacttcgt
      121 gcaattacag attaattatt tagactggga taatgaaagc atacaatcta gaaaatgcta
      181 cgaggtggca cagaagcata ataaacctgt aattgttatg gagccagtaa aaggaggtac
      241 acttgcaaaa ataccagaaa aagcagagaa attattgaat gactacaatt cagatatgtc
      301 aattccatct tgggcaatac gttttgcagc aagtaaagat aatgttatga tggtcttaag
      361 tgggatgtca aatatggaac aattattaga caacacagga tatatgcaaa atttcaaacc
      421 attcatacaa gaggaatata atattataga tgaagcagta gggattatca acgaatcaat
      481 tttagttcct tgtactgcat gtcagtattg tgtagaaggc tgtccaaaaa atatcgctat
      541 tccaaactat tttgcattgt acaatgcaga aaaacaagct cttaatactg gattttcaac
      601 acagatggtt tactataata attatactaa aacttatgga aaggcttcag attgcattga
      661 atgtaaacaa tgtgaagaaa gttgcccaca acacattaaa attatagatg ctttaaaaaa
      721 tgtagcagaa atctttgaga aataattcaa tctatacaat atcaagaggg gaagatatat
      781 atgacaattt cagaagttag taaaaaatac gaactttcag cagatacttt acgatattat
      841 gaacgaattg gtttgatacc acctgtaaat agaaataaaa gtggaattag gtcttttaca
      901 gaagaagatt gtgagtgggt taatttcata aagtgtatga gaggtgcagg tctttctatt
      961 gagacattga ttgagtatgt agctatgttt caacaaggga gtagcactat acaagctaga
     1021 aaagaacttt taatagagca gagaaatcaa ctagcaaaaa gaatggaaga aatgcaaaaa
     1081 actttggaac gattaaattt taagattgat agatacgaag aaggaattat tgagaaagaa
     1141 aaagtattaa agagcagtag aaataagaaa atggaggaag tatcttatga gtaaacatgt
     1201 attgatagta tcagctagtc ctagaaaagg tggaaattct gatttattgt gtgaccagtt
     1261 tacacaaggg gtattagaat ctaaaaatca agtggagaag atatttttaa aagataaaga
     1321 tattaactat tgtactggat gtggtgcttg ttataaaaaa gaggcaagtt gcccacagaa
     1381 agatgatatg ggagaaatcc ttgaaaaaat gattgaggca gatgtgattg ttttagcaac
     1441 tccagtttat ttttatacta tgaatgcaca aatgaaaact atgattgata gaacttgttc
     1501 tagatatact gaaatatgtg ataaagattt ctattttatc gtttcagcag cggatgaaaa
     1561 tatagctgat atggaaagaa caatagaagg atttagagga tttacatcat gtttaaatgg
     1621 ttccaaggaa aaaggtatag tatatggtgt aggggcttgg aatataggtg atattaaggg
     1681 aagtgaggca atgaaacagg catatgaaat gggtaaaatg gcatagtata tttgtataaa
     1741 atagaatata tttttatcat ttcaacaata aaaaagaggc ttatctccat tttgagacag
     1801 cctctttatg ttatatcatt tatttttatt ctgtatcctt ttatatcttc tattttttga
     1861 tgtctattta agtatctgtt gttactcata tattttaata attaccctta aaaaactata
     1921 cagattcctg cataatatta ttctctttag taattaattt ttctaggttt ttccaatcaa
     1981 tttcttccat atttgcaaaa taatgaccac ttcctatgca agttaggtaa gactttccat
     2041 ctaattcaaa cgaagaacca ccaatacact ttgaattaca ttttttacaa gtacgagttt
     2101 tagttatgct atttttaata gttttagagc atttctccac agcatcctta tacgtattta
     2161 aatgaaaaag atttgcttta actctccatt tcttttcatt acattcaata gtaaataagc
     2221 tacgttttgg cttcttagtt gaatatacta ttttgtaaga acgagcacct gtctttggtt
     2281 caattttaca atctaatgat ttaccaagtt cgtgtaaatg acatgcaata tcataatctt
     2341 taccacttag tacttctaac atttcttctg ttgtctctgc tgttaattca cttttgttca
     2401 tattaaaaaa ttccccctat ataaataata atttcatata aaaataatta atatcagtat
     2461 attaaatgaa attattaaaa tcaatagaat tactataatc ttacattatt gggcatagaa
     2521 tgagagctaa tgatttatca gaaataagac ttaatagtat gaaaatttac ttagacgtgg
     2581 tacaattcta taaaagattt aaaactagga aaggagaaag ttgttgagat atagccaata
     2641 taacaattta tttatagatt gcaaatttat tatgagatta attagattgt taagtaagac
     2701 tattaaaagg tagtttttgg gattctcagc aacagtggta ggatattgat aattttttgg
     2761 ttactattat atttagctat tatattttcg ataggttcta tgataactag ggtaaataag
     2821 gtatgggtaa aggttttatt atttgtaata gtgggattgt caactatagt tctttcatat
     2881 gtaataactt gataatttta gagtaaaaaa caactttaat ctggaaattc tcaaaacaag
     2941 aattctcatg ttggtaaatt tctagatatg agaaaaaatt tgacattata aaaaagtaat
     3001 tttaaagcat ttttttacga aaaaattcag aagcaactat tttgaatgac ttcacaaaat
     3061 tttatatata aatgaagttt ggcatgttaa ttgctttaat aaaagttaac aagacacaaa
     3121 taaatataat catctaagta ttgattattt gaagttttag atttgaaatt taatctaaaa
     3181 gttcgtatta atattggtgt ttaggaaatt ttattttcct gtaaatataa cttaataata
     3241 ggaggtgaag tcatgtcaaa agtagtaata gctacagaga aagctccagg agcaattgga
     3301 ccatattctc aagcaattca agttggaaac atagtttcta catcaggtca acttccaata
     3361 gatatgaaga ctggcgagtt agtatctgac ccaaaagaag caacaaaagc ttctttagat
     3421 aatgtaaaag caatattaga acaagctggt acaagtatgg ataaaatatt taagactaca
     3481 gtattcgtaa aagatttaaa ggattttaat gatattaatg aagtgtatgc tacatatttt
     3541 aaagaaaatc caccagcaag gtcatgtgta caagttgctc aattaccaaa agatgcagtt
     3601 ttagaaattg aagcaatggc agtattataa gtattataac aataaaacca ataacaaaac
     3661 taatgaaaat taaaaactaa taaaaaaagt ctacaattaa aaaagtttat attaaaaaag
     3721 tctatataaa aatctatcta aatatttaac tattgagctt ttctcatata atgtatattt
     3781 ttatgttaaa aatatatttg ttatacgagg aaagcttttt ttattttatt attgtattgt
     3841 gatttatttt attgtctgca tattataatt aagattacta tataaaacaa actttttata
     3901 aattaatatc atgtgtttta gcgcaaatat ataaataagt aatgttttat atatatttta
     3961 aagagtgact atatgttaaa atatagctaa actgataatt tggtaaatta aattctaatg
     4021 ggggaaacta tgttaagtaa attaaaagaa tttcaacaag aaatgataaa atacactgaa
     4081 acagtagcga gtgtgttaga tgtagatatt gaaatagttg atgatagatt aataagaatt
     4141 agtggcacag gtctgtataa atcaaagata aatgaaagcg ttgttacaga aggctttatt
     4201 tacgacaatg ttatacagac aggtcaggaa ttagtcgtat tggatatatg tgataatcag
     4261 ctttgtatag aatgttccca ttacatgaaa tgcctaaata aagtaataat agcagttcca
     4321 ataaagtata ataatcgaac tataggagta attggtgcta ttagtacaga taaaactaaa
     4381 aaagtagaaa taagtgcaaa aatagataat tatttaaaat ttgtcaatca tatatgcgat
     4441 ttaatttcta tgaaaataga agaacatgaa gtgtcaaaaa atagcagtag aaaaatggat
     4501 atgatgatag aaatcattga aaatgttgaa aaaggcgtta taatactaga cataaatagc
     4561 aaaatatctt atataaacaa tatagctcta aaaaaattag atatagataa aaatataata
     4621 gaaaatatag taaatatagt atctgttgaa tcttcctcaa atggacatga attactagaa
     4681 attgatattg ataacaagat ttataatata aatgccaaaa taattccagt atatccatat
     4741 ataaatcaat acgacaaaat aataatattt gataagacat atatcaatca taaaggacat
     4801 gtaaaagtta atagtggctg gggaaatagt gatatagagt ctataattgg aaattcagaa
     4861 gcaatgttaa aagtaaaaga gagaactaag aaattagcaa agtcaaattc aactgtactt
     4921 ataactgggg aaagtggcac gggtaaagaa ctaattgcta gagctataca cgctgagggt
     4981 agtagatgga ataagccatt tattgcgata aactgcgctg ctattccaga aaatctcctt
     5041 gaaagtgagc tatttggcta tataaaaggt gcatttagtg gagctagtag tggaggtaaa
     5101 gtaggtaaat ttgaacttgc aaatgaaggt gttatatttc ttgatgaaat aggagattta
     5161 tcaatgcctt tacaagcaaa gttacttaga gttttgcaag agagaaaatt tgcgagaatt
     5221 gggtcaaata aacttataga tttagatata agagtgattg ctgctacaaa taaaaattta
     5281 ttgaaattag taaatgaagg taagtttagg gatgatttat actatagatt aaatgtaatt
     5341 cctataaatt tgccacctct tagggaaaga aaggatgata ttgaggcaat aatgatgaag
     5401 tttgcttcta aatattcttt agagttaggt attcagttaa ataaaataga agaaaatgta
     5461 atgaatatgt tgattaacta taattggccg ggaaatataa gggaactgga aaatgcagtt
     5521 gaatatatga tgaatttggt tggagatgat ggaataatat ataaagatat gcttccattg
     5581 gatattttaa attattataa tattaatgga aacatttgta aaaataagga tataaatata
     5641 atatttgaag atgacatagt tggaggaata gtagagaatc aagaaaggat actttcaatt
     5701 aaagaattag agttgactta cataaataag ctgttgaata agtatggaag agatacgaag
     5761 acgaagaaga aaatagcaaa agacttaggt ataggtttag ctacattata taggaagttg
     5821 gaagaagaac agtcgtaata taatagaaaa ttaaaaccat aaatctttat atattttaaa
     5881 actaaataaa atacacatat aattaaaata gaaataaaac ataaacttag aactaaataa
     5941 aatataatca aataaaatta cagaaggtta tagtcatata tttatagaaa ataaaagtag
     6001 taatatatct gataaaacaa tagacatata aaaaataaga gcataataaa ggaaaaatta
     6061 agacaaataa acgatttctc gaattgataa ttctcgtatt gataaaaatt tcccaatatg
     6121 agaatttttt cttttataaa tcttgttttt tgcactcaaa acttataaac tttaaagaaa
     6181 ttttataaaa gtatgtaatt ccaacataaa aaaattgttt ttagaataaa taatattggc
     6241 atgctaattg ctttactata agacacaaag ataaaaaatt aaaatgacat cgaaaaattt
     6301 gaaaggggaa tataaaataa atgagagaaa taaaatggaa attaaataac ttacctaaat
     6361 cagaggaaaa agaaaaagga attgaatttt taagagatga agagattgca aaggctaaag
     6421 cttttcatga gagctttcca caatacgaaa agactccttt agttaaatta gataacttag
     6481 caaaatcact aggcgtaagc ggaatatatg ttaaagacga atcttataga tttggtttaa
     6541 acgcatttaa agttttaggt ggttcttact ctatgggtag atacctagct caaagattag
     6601 atacagacat atctgagcta ggttatgata aattaacttc agatgaaata aaagaaaagt
     6661 taggagaaat aactttcttt acagctacag atggaaatca tggtagagga gttgcttgga
     6721 cagctaataa attaggtcaa aaatctgttg tattaatgcc aaaaggttct tctgaattta
     6781 gattaaataa aatcaaagga gaaggtgcag aagctagtat aacagactta aactatgatg
     6841 atgcagtaag attagctaat gattatgcag aagctgatga ccatggagta atggttcaag
     6901 atacagcatg ggacggatat gaagaaatac cagcatggat aatgcaagga tatggaacta
     6961 tggctaaaga agctatagag cagttaaaag aatatggagt agatagacca acacacgtat
     7021 ttgttcaagc aggagttggt tcacttgctg gtgcagttca agggtatgtt gcttcaatat
     7081 atgatgaatg cccaataaca gtagtagtag aggctgatga ggctgattgc tactataaat
     7141 cagcagaagc tggagatgga aaaccaagat ttgttggagg agatatgcca actataatgg
     7201 cagggcttgc ttgtggagaa ccaaacacta taggttttga agtgttaaaa aattattcat
     7261 cagcattcgt ttcagcacca gactgggtat ctgctaaagg tatgagaata ttaggaaacc
     7321 ctctaagagg agatgagaaa gtaatctctg gagaatctgg agcagttaca actggagcat
     7381 tagtttcaat ccttgaaagt gaagatttaa aagacttaag agaagctttg aaactagatg
     7441 agaactctaa agtattatta ataagtacag aaggagatac tgaccctgat aaatatagag
     7501 atatagtttg gaatggtgaa tgccaaagta aataaaagac ttaaggaaaa gaaacattta
     7561 acaaataaaa gaaaaattta agcaaatgaa aaactaaagc aaatgaaaaa ttcaaaataa
     7621 aaactaaata aaattaaaat aaagtctaaa taaaaattta ataaataaaa tataaaggaa
     7681 taaaaaatca atagattaag tgaaatgcta ataaattaaa aaagtgaaat atcaaaataa
     7741 aacgtcaaca aattaaaaat tatatggggg aatatcaaaa tgttaaatga aacaagaaaa
     7801 aaacaattaa ctgaagtatg ccaagactta ataagaaatg ctagttattc aggtcaagaa
     7861 gaaaacgtag taaaagctat agaagagaat tttgctttac taggttttga tagttggtca
     7921 agagatagat atggaaatat agtaggatgt ataaaaggaa ataaaccagg taaaaaaata
     7981 ttatttgacg ggcatataga tacagttcca gttccagatg caagtaaatg gagtgttcct
     8041 ccttttgaag cgagaatagt agatgggaaa atatatggta gaggaacttc tgatatgaag
     8101 gggcaagtaa gtgctatgat ggctgctgtt tcatactttg cagaagatac taataaagac
     8161 tttgaaggtg aattgtacgt agcaggtgtt gttcatgaag aaatatttga aggagtttca
     8221 gctagagaga taagtaaagc tgttcaacct gattatgttg taataggtga atcttcagaa
     8281 ttaaacttaa agataggtca aagaggtaga ggagaaatag tagtagaaac ttttggtaaa
     8341 ccagctcact ctgctaatcc agaaaaaggt ataaatgcag tttataaaat ggctaatgta
     8401 atccaaaaga tacaacaatt agtacctcca actcatccag ttttaggtga tggaatatta
     8461 gttttaacag atataaaatc ttctccatat ccaggagcat cagtagtgcc agattattgc
     8521 aaagctactt ttgacagaag attattagtt ggagaaacaa gagaaggagt attagctcca
     8581 atacaagctt tattagatga aatgatgaaa gaagacccag aattaaatgc aaaagtttct
     8641 tatgcagttg aaaaagctga ttgttacaca ggaaatacta tagaaagtga aagattcttc
     8701 ccaggttggt tatatgacga agaagatgaa tttgttcaag cagcgtataa gggactaaaa
     8761 gaagctggaa tagattcaga aataactcaa tattcattct gtacaaatgg aagtcactat
     8821 gcaggtgagg ctggaataag aactatagga tttggacctt ctaaggaaaa tcttgctcat
     8881 acaatagatg agtatataga attagaccaa ttattcaaag gtgcagaagg atattatggt
     8941 atattaaaat cagtttataa aaaataatta ggagatggtt ttgtgagaac tttaataaaa
     9001 catgggctta tagtagatgg aaataaaaca ccagcatatg aaggggatat tttaatagaa
     9061 aacgagaaaa tattaaaaat atcacaggat ttaaacgaag aagctgataa agttatagat
     9121 gcaaagggta gagttatatg tccaggtttc atagatactc atagccattc agacctagtt
     9181 attttagtta atccatataa tgaggtaaag attagacagg gaattacaac agaagtgttg
     9241 ggccaagatg gtatatctat ggcccctcta cctcaagagc atatatcttc ttggagaaaa
     9301 aatttagcag gtctagatgg tgaaagtgat gaaattgatt ggaaatatga aacaacagag
     9361 aattatttaa aaatgatgga tgataacggg gtaggtttaa atgaaactta tttagtacct
     9421 catggaaacg ttagaatgga agcaatggga cttgaagata gacctgctac taaagaagaa
     9481 attcaaaaaa tgtgtgaaat cacagagaga gaattaaaag caggagctat aggattgtct
     9541 actggactta tatatatacc ttgtgcatac tcattgactg aagaaattat agagatgtgt
     9601 aaggtagtag caaagtatga cggagtattt gtagttcacc aaagaagtga agcagataca
     9661 attttaacat caatggaaga aatcatagaa attggaaaac aatcaggagt aaaagttcat
     9721 ttttcacatt ttaaggtttg tggtaaagca aactggaagt atataccaca agttatagag
     9781 cttttagaaa aagcagaaaa ggaaggcata agagtatctt ttgaccaata tccatatgca
     9841 gcaggaagta caatgcttgg agtagtttta cctccttggg cacattctgg aggaactgat
     9901 aagttaatag aaagattaag tgacgaaaat gaaagagcaa aaatgaaaaa agatatagca
     9961 aatggaatag aaggttggga taattttata gaatttgctg gaatagacca aatatttgta
    10021 acaagcgtaa agacagaaaa gaataaggat gtaattggta agagtctatt agaaattgga
    10081 aaaatgagag gaaaagaccc attagatgca acgtttgatt tattaaaaga agaagaaaat
    10141 gcagttggaa tggtagattt ctatggatta gaagaacata taataggatt tatgaaaaga
    10201 gacgagcaaa atgtgtgtac agatggtctt ttagcaggta aaccacatcc aagagcttat
    10261 ggttctttcc ctaaaatact aggaagatat gttagagaat tgaatgtact aacaatcgaa
    10321 gaagctgttt ataaaatgac taaaaaagca gctacaagct ttagtataag agatagaggc
    10381 gaattaaaag aaggatattt tgcagatata gttatatttg acaaggatac agttagtggt
    10441 tgtgatgatt ttatcaactc tatgcagtac cctacaggta tagattatgt aattataaat
    10501 ggaaattgtg ttgttgaaga aggaaaatac aataaaatta aagcaggaaa agtacttaaa
    10561 aattaaatct tagctttaag gaggaataaa tttatgattt tagtggggaa tgggaggcta
    10621 ataagccaag attctttaaa tccctatatg gaagatggat gtgtagtaat aaatgataat
    10681 ctaattgaag atataggtac tacaaatgaa atgaaagcta aatatccagt acatgaattt
    10741 atagatgcag gtaagaaaat aattatgcct ggtctaataa attcacatat gcatatatat
    10801 agctcatttg ctagaggaat ggcagttcca ggaaaacctt ctgagaattt tttagagata
    10861 ttaaacaatt tatggtggag acttgataaa cagcttacat tagaagatac aaaatacagt
    10921 gcatatgcaa cgtatataga atgtataaaa aacggtgtaa caacagttta tgaccatcat
    10981 gcaagtccaa atgcaattga aggcagtttg tttacaatat cagatgttgc aaaggatttg
    11041 ggaattagaa catgtctttg ttatgaagta tctgatagag atggtggaaa tacaatccaa
    11101 caaggtatca atgaaaacgt aaactttata aaatacgctc aaaaagatga tacagacatg
    11161 ataaaaggaa tgtttgggct tcatgctgca tttacactat ctgatgatac attaaataag
    11221 tgtgcttcag agatggctgg tcttgataca ggttaccatg tacatgttgc tgaaggacta
    11281 gcagacttac aatataatgt agataaatat ggaaaaagag ttttagaaag attcaatgat
    11341 tttggagtac taaaagaaaa aacaatagca gttcattgta tatatataag ttcagaagaa
    11401 atcagtcttt taaataaaac aaatacattt gtagttcata atccagaatc taatatggga
    11461 aatgcagttg gatgttctcc tgcaatagag ttattaaaaa ggggtgttac agtaggtcta
    11521 ggaactgatg gatatactag cgatatgttt gaatcattaa aagttgcaaa tataatccac
    11581 aaacatcatt taaaagaccc aagagttgga tttgaagaat cacaaacaat gttatttgat
    11641 aacaatagaa aaatagcaaa agcttattat aaaaatgacc taggagtact taaaaaaggt
    11701 gcttatgcag atgttatagt agttgattat ataccacata ctccattgac agaaaacaca
    11761 ataggaggac acactatttt tggaatgagt ggaagaagtg tagatacaac tattataaat
    11821 ggaaaagtac ttatgaaaga tagaaagtta ataaacatag atgaagaaag tattctagca
    11881 aaaagtagag aattaagtca aaatctatgg aatagaattt aaataattga tattaatata
    11941 caagtaagaa acaagaattt agatgttaaa agcagagctt aaacaaaaat aggaatttaa
    12001 atgaagtata tgaataaaaa atatacggaa tagaatatta aattaaatat agaagggaga
    12061 cccaaaatgt ctcaagataa aagccctaaa atagctccag tagatgaaag gttacctgta
    12121 tccaaatctt gggtattttc actacagcac gttttagcaa tgtgtgcagg agcagtagca
    12181 gttcctatga tgataggaag tgctgcacga ctagatcatg ctagtattgt ttttttaata
    12241 aatgcttgct tgtttatggc aggtatagga acactcatac aaagtgttgg tataggaaat
    12301 attgtaggtg ctaaaatacc agtaatagaa ggaactagtt ttgccgctgt ggcaggtatg
    12361 acagcaatag cctcatcata tagtgattca caagttgcaa tacagacagt atttggctca
    12421 gtaatagtag ctggattgtt ttgtatacta cttgctccag tatttgaaaa actaataaga
    12481 tttttcccta gtgtagttac tggaacagtt gttatggtaa taggtatttc actatttcca
    12541 gttgggataa aatggataac tgacaacaaa ttggaagcgg ttgaaccaaa ctcattagca
    12601 ttagctttag cagtattggc aataacttta ctttcattta aattcttaaa aggaatttgg
    12661 aatagcacag ctatattggt aggtatagta cttggtacac tactttccat ggcagttgga
    12721 atggcagatt ttagtgcagt aaatggagtt ggttggctta acttaaatgt acctttaaga
    12781 tttggaatgc ctagatttga accatctgca atactatcat tgtgtttaat tatgttggta
    12841 cttatgacgg aatcagttgg taatatgata gctattcatg aaatggtaga caaagaagta
    12901 acaggcaaaa atattaaaag aggtctattt ggagatggaa tatctacagc attatctgga
    12961 atattcaatt catttccaat aactccattc ggtcaaaata caggaatagt aggtcttaca
    13021 ggtataaaat caagatttat agcagtttac gcaggggtta tattgctagt acttggattc
    13081 ttaccaaaat ttgcagcaat aataggagca atacctaagc ctgttcttgg aggcgtagga
    13141 tttgcaatgt ttggaatggt aatggttgga ggagtaaaaa ctttaagcaa agtaaaattt
    13201 gatggaacta aaaacggggt tatagtagca gttagtgttg gtttgtctat gataccacta
    13261 acaaatcctg atttctatca taatttccca acttgggtac aaacaatatt ccacagtgga
    13321 ataacaacag gtagtttatc agcagtgatt ttaaacatat tcttcaatga actaggttct
    13381 aaaaaagaaa aaaataacaa agttgaagaa gcaaagtaaa aataagacaa gtaaaaatct
    13441 atatttatta aaagtcatta agcattgata aaagtaattc agagatatta ataattcaac
    13501 aaaactatct tatattttaa ataataaaaa agtaaataaa taacgaatac aaataaaaaa
    13561 atacaatcta aatattaaaa atatttaatt taagggagag ataatgatat ggctaattta
    13621 aaagttaatt tatatggaat ggaattttta aacccaatta tgacagcagc aggtcctgga
    13681 gcaaaagatg gtgctctctg tcaagaggca gcaaaaggtg gagcaggagc tatatgtaca
    13741 aaaactatat ctgtattacc agctgatgta ccaagaccat gtatggcaaa aactaatagt
    13801 ggtttcttaa acacagaatt atggtcagag cttccaaaag agcaatggat agaagaagaa
    13861 tacaaaaagg taaaaaaaac agggcttcca gtaatagtaa gtatgggata ttcagcagag
    13921 caaatagctg aggttgctcc tttagtaaaa ccttatgcag atgcggttga attatctact
    13981 cattatgtag gtacagatgt tgcgccaata gtaaatgctt taaaagctgc aaaagctgtt
    14041 ttagatgtac cagtatttat gaaaatgagt cctcatactg acattcaaac tattgcaaaa
    14101 gctgttgaag aagcaggtgc agatgggctt gtaatgataa actcatttgg accatgtatg
    14161 gcaatagatg taaatacagg ataccctata atgggaagta aagctggata tggatggtta
    14221 tcaggtgcac caataagacc tctagcaata agatgtatat acgaagcttc tcaagttgta
    14281 aatataccaa taatcggtgt tggtggaatc acttgtggaa aagacgtagc tgaaatgatg
    14341 atggctggag caagtgctgt tcaagtatgt acagaagcaa tccttaaagg accttctatc
    14401 tatggaaaaa ttgctaaaga attaaatgaa tttttagatg aaaatggata tgctgatgtt
    14461 aatgaaataa aaggattaac tcataagaaa atgacagcta gaggatttag aactcattca
    14521 gtagctccag tagtggatga taacaagtgt atcaaatgta agcaatgtgt aacaagctgt
    14581 gtatatgaag ctttagaagt tacagataag ttgaacatag atgcagacaa gtgttttgga
    14641 tgtggattat gtgtaactag atgtccaaaa ggagctttaa gcatctgcta caatcaagta
    14701 gaagctatga atgagtaata attatataga attttggtaa ttaacttaga gtaataggta
    14761 ttaggataat aaaatatatc agataaacac gcattatgca ttaataatac agtctaaagt
    14821 taataatttt atgatattga tttaactaaa tattagttta gctgaatatt aatttaatta
    14881 ggtattggtt taatcttaat atagatttac tacgtataag tgaactaact agattacttt
    14941 aagttaattt tttcatataa aataaattga tacgttataa gtaaggtgga gtctatatgg
    15001 gaattgtttt aaaaggtggg aaagtagtaa caagtgaaaa aacttactgt gcagatgtaa
    15061 gaatagaaga tgaaaaaata gtcgatatag gtcttgatat atcaaaagaa aatgatgaaa
    15121 tagttgatgt tagtgattct tatgttatac caggaggtat agatacacac actcactttg
    15181 acttagatac aggtgctact aagacagcag ataactttga gacaggaaca aaagctgcta
    15241 tagtcggagg aactactact atattggatt ttgctacaca ggctaaggga tgttctttga
    15301 aagaagggtt agaagaatgg catgaaaaag ctaaggataa atgctattca gattatgggt
    15361 ttcatatggc tataacagac tggaatgact cggtttgtaa tgaaatggaa gatatggtga
    15421 aagagggagt atcttcattt aaattgtata tggcatataa aggaagtctt caagttgatg
    15481 atggtgtcat atttgaagcc ttaagaaaag cagaagagat aggtggaatt attggttttc
    15541 attgtgaaaa tggggatata atatgtgaac ttgtggataa agcaaaggca gaaaataatt
    15601 tatctccaaa atatcatcaa ttaactagac cagctatact ggaagcagaa gctacatcaa
    15661 gacttatgaa gatagctgaa gttgcaaact ctaaagtata cgtagttcac ttaagttgta
    15721 gagaatcctt agaggaggtt ctaaaagcta agcaaagagg tgtggatgta atagttgaaa
    15781 cttgtcctca ataccttcta ttaaatgatg gattatatga tttagatgga tttgaaagtg
    15841 ctaagtatgt aatgtcacca cctttaagag ataaagaaaa taatgaaata ttatggaatg
    15901 ctatttcaga taatcaaata gatactatag gaacagacca ttgttctttt aattataggg
    15961 gacaaaagga acttggaata aacgatttca gtaagatacc aaacggttct ccaggtgtag
    16021 aacacaggat gagtctttta tacacatatg gagtctgtaa aaataaaata tctatcaaca
    16081 agatggtaga agtaacatca actaatgcag caaagttgtt tggaatgtat cctaataaag
    16141 gagccataga aataggcagt gatgcagata tattagtctt aaatccaaac aaaaaatcta
    16201 aaatagaagc aaaaaaacaa ttacaaaatg tagattatac accttatgaa gggtatgaag
    16261 tggattgtca aatagaacat gtttatttaa gaggaaaaga agtagttaga gatggagaat
    16321 taatctgtaa tactccaagt gggagatata taaataggcg tctaacaaag ggggctagag
    16381 aatggtagaa tttatattaa atggagagaa agttgttgca aaagaaggca aaaaactttt
    16441 agacttcctt agagaagata tgaatataac ttcagttaaa aatggatgtt cagaaggtgc
    16501 ttgtggaaca tgtatggtta taattgatgg taaagcgact aaagcatgtg tatttaaaac
    16561 tgataaatta gctgggaaaa acataacaac tatagaggga ttatcagaga gagaaagaga
    16621 tgtttttgca tattcttttg ctagtcaagg agctgtacaa tgtggatttt gtattccagg
    16681 tatggtaatt agttcaaaag cacttataga cgttaataat aatcctactg aagatgatat
    16741 aaagaaagct ttaaaaggta acatatgtag atgtacaggg tatgtaaaaa taattaaggc
    16801 tgtcgattta gcagcaaaga tttttagaga aaatatagat gtgccaaaag taaactgtaa
    16861 aggtctagta ggtgaaaatc ttcctagagt tgatgcagtc ggtaaagcac ttggatattc
    16921 tgaatatgta gatgatatga gaattgaggg tatgatttat ggttcagcag ttagagctaa
    16981 gtatcctaga gctttagtta aaaaaataga tatagaagaa gcaaaagctt atccaggtgt
    17041 tgttgcagtt ctaacagcta aagatattcc aggaactata aaggtaggtc acttaaaaaa
    17101 agattgggac actttaatac cagaaggtga aataactaga tacttaggag atggaatagt
    17161 tttagtagca gcagaaacta gagaagcgtt agaagaggcc aaaaagctag ttaagataga
    17221 ttatgaggaa ttaacaccat taagtactac agcagatgct cttagagaag atgctccaaa
    17281 gatacatgag acaggtaata tacttgttag ggaacattta gtaagaggaa acgctgaaga
    17341 aaagatagaa aattcaaaat atgttgtaac taaaagatac actacacctg ctacagaaca
    17401 tgcattttta gagcctgaat gtgcagtagc tggtccttat gaagatgatg gagtaataat
    17461 ctattcaaca gaccaagggg tgtttgccac tcaacatgaa tgtgcagata tgttagggtt
    17521 gccatatgag aaggtaagag ttataaacaa aatggtaggc ggaggatttg gtggaaaaga
    17581 agatatgagt gttcaacacc atgcagcttt acttgcatac catactaaga gatttgtaaa
    17641 agtacagtta acaagaaaag aaagtattat tgttcatcca aaacgacatg ccacagaaat
    17701 ggaatttacg acagcatgtg atgaaaatgg atacttaaca ggtatgaagg caacgataat
    17761 atctgataca ggtgcgtatg cctctcttgg aggaccagtg ctacaaagat tatgtactca
    17821 tgcagcagga ccatacaatt atcaagatat tgatataaca ggaacagcag tatatactaa
    17881 taaccctcct ggaggagcat ttagaggatt tggagttacg cagagttgtt ttgcaacaga
    17941 atgtaatcta aacttacttg ctgaaatggt tggaatatca ccttgggaga taagatataa
    18001 aaatgcaata agacctgggc aagttttacc aaatggtcaa tttgcagatg aaggaacagc
    18061 aatagcagag acactagaag ctgtaaaaga agagtatgaa aagcatccac gtgcaggtat
    18121 agcttgtgcc atgaaaaata gtggagttgg agttggtata cctgatgttg gaagatgtag
    18181 attggttata aaagatggca aagttcacat aagaacaagt gcttcttgta taggtcaagg
    18241 gcttggtact gttgtgactc aagtagtttg tgaaactgct aacctacttc ctgaaaatat
    18301 agtgcatgaa gttccagata ctcatttaac accagactct ggtaatacta cggcttcaag
    18361 acagacagta tttacaggag aagctactaa ggttgcatca agtaagctaa aagaagcttt
    18421 agactctggt aaaactttac ttgatttaga gggagaggag ttttatggag aatatgaaag
    18481 tattacagat aaaatgggtt gtgacaaaga aaatccagtg agtcatgttg cttatggata
    18541 tgcaacacaa gttgtaatgc tagatgaaaa tggaagattg gaaaaagtag tagcagcaca
    18601 tgacgttgga agagctataa atccaatcag tttagagggg caaatagaag gtggagtagt
    18661 tatgggtctt ggatatgctc taaccgaaga ttacccagta gttaaatctg tccctacagc
    18721 taagtttggt acattaggtt tatttaaatc tacagatata ccagaaatac aagctttaat
    18781 agttgaaaag aacaaagatt tattggctta tggggcaaaa ggtgtaggag aaatttgtac
    18841 agttcctact gcaccagcag tacaaaatgc ctattatgta tttgataaag aatttagaac
    18901 tgaactacca ctacaaaata ctccatatag taaaaaaaag taacaaaaaa aagaaatatt
    18961 aaataaaaaa atagatgaaa ttgtaaataa aaaaaaccct tttgcataag gtatatttta
    19021 aatgatagaa tatacatata caggagggtt tttcttttat aaaataagtt taataaaatt
    19081 gagaggtgag cagaatgaat tttaataata gtctagaatt aactcagtct cagaagctaa
    19141 taatgaccac acaacttaag caatcattat caatattaaa tatgagtaag ttagaattag
    19201 aagaagagat taaaagagaa gcagagaata acccattggt tgaagttgag aagagtgggg
    19261 agataaattg ggaagaatat ataaaagata tggataggtc aagacgttta gatagaacag
    19321 agataagcta taatcctgat aatgaagtta atctagaaaa tttagttaaa tattcgtcaa
    19381 atttatatga ggatttaaaa tttcagataa gcctgtataa acttacagat aaagaattgg
    19441 aagtctgtga gtatataatt gatagtttag atgaggatgg atatttaaga actgaggaaa
    19501 aagtaattgt tgatacctta aagatagatg aagaactttt tgaaaaatgc ttgattagtg
    19561 ttcagcagtt ggaacctagt ggagtagggg caagaagtct ttcagaatgt ctgatgatac
    19621 aaatggctag tttgggaata tataatgaaa ttttagagga aatcgttaca aaagacttaa
    19681 atttaattgc aaataataaa tacaaagaga taagtaaaaa gtacaatatg tcattacaga
    19741 aatgcgttga actaataaac ataattaaga cattagaccc caaaccagga agaacttgtt
    19801 cagtagagaa aagtgtatac attcaacctg atgtaacagt agaaaaaatt gatgatgaat
    19861 ttattgtata tttaaatgaa aaagattctt atcaaataag aatcaatagt tattataaag
    19921 acatattaaa aaattcacag tctgatgaaa gtgcaaaaga atttataaaa gaaagactaa
    19981 attcagcaac aggacttatg aaaaatatag agagtaggaa aactacagta cttaagattg
    20041 cagaagagat agtaaaagct caagatgaat ttttgagaaa agggacaaaa tatataaaac
    20101 ctttgaaaat gaaagacata gcagaaaaat tagaatttca tgaatctact ataagtagag
    20161 gtgtaaatgg taaatatatg ctaacacctt ttggtgtata tgaatttagg tactttttca
    20221 gtagtgccat tgagactgag aataatgaaa tggcatctag tacaagcata aaaaaaatta
    20281 taaaggaaac aataaaagac gaaaataaaa agaaaccttt aagtgatgac catatatcga
    20341 agttactaaa agaaaaagga ataaatgttg caagaagaac agttgctaaa tatcgtgaag
    20401 aattaggaat cccatcttcg agtaaaagaa aagaatacta ataagtaaaa gacaaatatc
    20461 agtatagtaa ggggaaaagg ctaattaaaa gatgaatgtg gattaggcta aaaattttat
    20521 gaaataaaat aataaaatat ggaaaagtat taaaatgcta taagatatta aaaaactggg
    20581 gaacattaaa acattaaaga acattagagc tctaaggaat attagaattt taaagaagat
    20641 taaaatttag aaaatataaa aatactaaaa agagctgaaa aactggggaa cattaaaata
    20701 ttgtaaaatg ctaaaaaatt agaaaacata attaaataag tgcttataaa gaatgattaa
    20761 attaaataag tgtttgtaaa gaataattaa atattagaaa aagaagaata cctagataaa
    20821 taaactatta ttaaatttaa taatcacaaa aggaaaacac atagggtata aataaaaatt
    20881 ttagctagac agaagtaaac ttagctggaa ataaaagtaa ctagaaataa aattatttat
    20941 agtaataaat attataacag aaaatattaa aataaaaaaa tatatataaa aagccttata
    21001 taaaaagaaa aaatactgtg atttgaaaat ggtggagaaa tattttataa aataaaaaaa
    21061 taaaaaagtt tttgaaaaac tattgaaatc tatacgcaaa tcctataaaa taaaaatatc
    21121 ttgtgggact taaataaaca ctagggacga aaaaagtccc gaaaggaaag caataaggag
    21181 tatgttatga agaacttatt agaaattcaa aagaaattga ttccacaggc aattgaactt
    21241 atggaaagaa gatactctat tttaagacaa atatctttaa gtgaaccaat aggtaggaga
    21301 acattatcaa atgtattaga gattagtgaa agaataatca ggtcagaaac agagttttta
    21361 aaagaacaag gacttataga cgtagctgta tctgggatga ctatcactaa agaaggttct
    21421 gatttgttag ataaacttaa ggacatcatg agtgacatca tgggattgtc taagttacaa
    21481 gatagagtca gagaaaagct tggaataaaa agggtaatac tagttcctgg aagctgtgat
    21541 gaaaatggaa gtttattaaa agatgtaact agatatggat gcgagtattt cttaggtatt
    21601 ttaaaagatg gagatatcgt ttcaataaca ggaggaagca ctatgttgga gttttcaaac
    21661 gttattaaaa ctgataagag atacccaaat tctacaatag ttccagctag aggaagcatg
    21721 ggaactgatg tagagatgca atctaataac atagttgctt caataagtaa gaaattacat
    21781 tctaattgca aacttttaca tataccagat gagttggatg aaagtgcaat gaaaactctt
    21841 agccaagtgc cagagattaa gacaactttg gagtatatta aaagaacaga cattttagtt
    21901 tttagtatag gtagagcaga tgtcatggca aagagaagaa aacttccaga agacaaagtt
    21961 gatgagatat tatctaaaaa tgcagttgga gaagcatttg gatattattt caacaaagat
    22021 ggagaaatag tttacaaact gaatactgtt ggaattgatt tagaaacagt caaacaagta
    22081 aaagaaacta ttgcaatctt tgcaggtact gaaaaagtag aagccttatt agccatatca
    22141 aatatgcata aaaacatggt tcttgtaaca gatgaagaaa gtgcttataa gatattgtca
    22201 tcattataaa agtcaagacc tagcacagag ctagaaaaca tacatttact ttataggagg
    22261 tagcgaaaat ggttaaagta gctataaatg gatttggaag aataggaaga ttagcattaa
    22321 gaaaaatgat ggagcaacaa gataagtttg aagttgttgc aataaacgac ttaacagacg
    22381 ctaaaatgtt agctcattta tttaaatatg atacagctca aggaagattt aacggagaaa
    22441 tagaagttaa agaaggtgct tttgtagtaa acggaaaaga aataaaagta acagcagaaa
    22501 gaaatcctgc tgacctacca tgggctgaat taggagtaga tatagtatta gaatgtacag
    22561 gattctttac atctaaagat aaagctgaag ctcatataca agcaggagct aagaaagttg
    22621 ttatatctgc accagctact ggagatttaa aaacaatagt attcaacaca aacagtgaca
    22681 tattagatgg ttctgaaaca gttatatcag gagcttcttg tactactaac tgtttagctc
    22741 caatggctaa agtattaaac gataaatacg gtatagaaaa aggattaatg actactatac
    22801 atgcatacac taatgaccaa aatactttag atggtcctca tccaaaaggt gatttaagaa
    22861 gagctagagc tgctgctgga aacatagttc caaacactac aggagctgca aaagcaatcg
    22921 gtttagtaat accttcttta aaaggaaaat tagatggggc agcacaaaga gtaccagttg
    22981 taactggttc aataactgaa ttagtttgta cattaggaaa aaatgttaca gttgaagaaa
    23041 taaatgcagc tatgaaagaa gcttcaaacg aatcttttgg atatactgaa gaaatgttag
    23101 tatcttctga cataatcgga ataagctacg gttcattatt tgacgctact caaacaaaag
    23161 ttatggaagt agatggaaaa caattagtta aagttgtttc ttggtatgat aatgaaatgt
    23221 cttatacttc tcaattaata agaactttag gatactttgc acaattagca aaatagtcag
    23281 ttttgagaaa atagaaaagt agattgacaa agtccgagtt tgtagtttaa tactacggac
    23341 tcaggacttt ttttaagaaa aaatatataa aatacaccta aataatgtta catactatat
    23401 ataaaaatat gataatatga tatatttaat agggtaaaag tatgtactat attatacttg
    23461 gcaataaaaa attactttat taaaaggaga gatatttatg tcaatgctta acaaaaaaac
    23521 tatagaagat attgatgtgt gcggaaaaaa agtattagta agatgtgact ttaatgtgcc
    23581 attacaagat ggtgtaatta ctgatgaaaa tagattgaac ggagctttac caactattca
    23641 atatttaatt tctaaagggg caaaagttat attatgctct cacttaggaa agccaaaagg
    23701 agaagctaaa ccagaattat ctttagcacc agtagcgaag agattatctg agatgttagg
    23761 aaaagaagtt gtatttgcag cagacgataa tgttgtagga gaaaatgcaa agaaagcaac
    23821 tgaagaaatg gaaaacggag atgtagtatt attagaaaat actagatata gaaaagaaga
    23881 aactaaaaac gaagagaatt tctctaaaga actagcatca cttgctgaaa tatttgtaaa
    23941 tgatgcattt ggaactgctc atagagcaca ttgctcaaca gttggtgcag gtgagttctt
    24001 acaagagaga gtttgtggat atttaattca aaaagaacta aaattcttag gagaagctgt
    24061 tgcaaaccct gtaagaccat ttacagctat acttggagga gctaaagttt ctgataaatt
    24121 agccgttata aatgaattat tagaaaaagt tgataatcta ataataggtg gaggaatggc
    24181 ttatacattc ttaaaagctc agggttatga agtaggtact tctttacttg aaatagacaa
    24241 agttgaatat gctaaagaaa tgatggaaaa agctaaaaat aagggtgtta acttattatt
    24301 accagtagac gttgttatgg cagaccactt tgcaccagat gctactccga tagtaacaga
    24361 agatgctaat gtaaaagaag gttacatggg actagatatg ggacctaaga ctatagcaaa
    24421 ctttgtaaaa actataaaag aatcaaaaac tatagtatgg aatggaccaa tgggagtatt
    24481 tgaatttgaa aactttgcaa atggtacatt atcagtagct agagctatgg cagagctaac
    24541 tgatgcaact actgtaattg gtggtggaga tagtgcagca gcagttaatc aattaggatt
    24601 tggtgataag atgactcacg tatcaacagg tggaggagca tcactagaat ttttagaagg
    24661 taaagaatta ccaggtattg cagcattaga taataaataa actaatatat agaggagatt
    24721 gtaaaatgag aaaacctata attgcaggaa actggaaaat gcataaaaca ataaaagaag
    24781 ctttagaatt tgtaaatgaa gttaaagata aagtaaattc tgataaagta gaagctgtta
    24841 tatgtgcacc atttacttta ttaaaggatt taaaagaagc tactaagggt acaaatataa
    24901 agataggtgc tcaaaatatg cactttgaag aaaaaggagc atttacagga gaagtttcac
    24961 ctcttatgtt aaaagaaata gatatggact atgttgtaat aggacattca gaaagaagac
    25021 aatacttcaa tgaaactgat gaaactgtaa ataagaaagt tttaaaagca ttagaagtag
    25081 gaatagaccc aatattatgt gttggagaaa ctttagagca aagagaagct ggaaaaacta
    25141 aagacgtttg tagagttcaa gttgaaaaag ctttagaaaa tgttcttaag gatgatttag
    25201 caaaagtagt tgttgcatat gagccaatct gggctattgg aactggtaaa actgcaactg
    25261 ctgaagatgc taatgacgtt atatcttata taagagaagt tataaaagga ttatatggag
    25321 aattagctaa tgaggttaga atacaatacg gtggaagtgt taaaccttca aatgtagccg
    25381 aaataatggg tcaaagtgat atagatggag ctttagtagg aggagctagc ctagctagta
    25441 atgattacct agaccttgtt aatttctaag gagtgaaatg atgaaaaaac cagttgcttt
    25501 aattattatg gacgggtttg gatacaataa ggatgttaaa ggaaatgcaa ttgctgagtc
    25561 caaaactcct aatttagata gaataaaaaa agagtatcca aacacattga taaatgctag
    25621 tggtcttgat gttggtctac ctgatggtca gatgggaaat tcagaagttg gtcacactaa
    25681 tataggtgca ggtagaatag tatatcagga tttaactaga ataacaaaat ctataaaaga
    25741 tggtgacttt tttactaata aagttttatg tgaagctatg gataatgcaa aagaaaattc
    25801 tcttcatgta atgggacttt tgtctgatgg aggagtacat tcacatatag accatcttaa
    25861 ggctataatc aaaatggcaa aagataaagg tgtacaaaag gtgtatgttc atgcatttac
    25921 tgatggtaga gatactgacc ctcaaagtgc attagaatac gccaaagaag tacaagctag
    25981 tatggatgaa attggagtag gtgaatttgc tacagtttct ggtagatact acgctatgga
    26041 tagagataag agatgggaaa gagtagagct tgcatacaat gctatggtta gaggtattgg
    26101 tgaaaaagca acttctattg aagaagctat tcaaaactct tatgatgatg gaaaaaatga
    26161 tgagtttata atgccaacag ttattatgaa agatgataaa ccagttggaa gtataaagga
    26221 aaatgattca attatattct tcaactttag accagataga gctagacaaa taactagagc
    26281 tttagtttgt gaagagtttg atggatttaa aagagaagat attaaaaact tctttgtatg
    26341 cttaacagag tacgatataa ctatagagaa tgtgcatata gcatttggac ctcaatcttt
    26401 agccaacact ctaggtgagt atctagctaa aaatggaaaa actcaactta gagctgctga
    26461 aactgaaaag tatgcacatg taactttctt cttcaatggt ggagtagaag agccaaataa
    26521 aggtgaagaa agactattaa taccttctcc aaaggttgca acttatgatt taaaaccaga
    26581 aatgtcagca tatgaattga cagataaagc tttagataaa ttaggagaag ataagtttga
    26641 ctttatagta ttaaactttg ccaatcctga tatggtaggt cacacaggaa gtatagaagc
    26701 agctgtaaag gctgttgaaa ctgtagacac ttgtgtagga aaattgatag ataaaatagt
    26761 agagttaggt ggaagtgcaa taataactgc tgaccacgga aatgctgaat atatgttaga
    26821 cccagaaaca ggaaaaactg taacagctca ctctattaat ccagtaccat ttattgtagt
    26881 tggtcaagaa tatgaaaatg caaagttatt ggatggagga agattatctg atatagcacc
    26941 aactgtttta gatatgatga agttagaaaa gccagaagaa atgactggtc attcacttat
    27001 ttcaaaatag tcgattatga taagtataaa ataagatata agctacaata taaatttaaa
    27061 aataacacag tttaggttta ttttgtaagg tctagatttt atgatataag atttagcttt
    27121 tgtaaggtct ggatattgta aggtgtagat gaaagtatat tttataatat ttacaatata
    27181 gacaatttat aaaataaaaa attataatat agataatgat ataaataata
//
LOCUS       DAMJVM010000044        26321 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.045, whole genome shotgun sequence.
ACCESSION   DAMJVM010000044 DAMJVM010000000
VERSION     DAMJVM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26321
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.045"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            257..595
                     /locus_tag="PC523_002022"
     CDS             257..595
                     /locus_tag="PC523_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2475546.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            617..934
                     /locus_tag="PC523_002023"
     CDS             617..934
                     /locus_tag="PC523_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2475547.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVSILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLILQIIDIIKK
                     NKI"
     gene            1084..1764
                     /locus_tag="PC523_002024"
                     /pseudo
     CDS             1084..1764
                     /locus_tag="PC523_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905814.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2105..2872)
                     /locus_tag="PC523_002025"
     CDS             complement(2105..2872)
                     /locus_tag="PC523_002025"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDF2475548.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKEDIIKEAKELKGVCLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYISDIPLLFTFRSLAEGGEKVISKDYYTTLN
                     KEISSTGLVDLIDVELFMGDEVIDEVINFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQDEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELSSVLNLLHKSIN"
     gene            3633..4265
                     /locus_tag="PC523_002026"
     CDS             3633..4265
                     /locus_tag="PC523_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDF2475549.1"
                     /translation="MTIPNSTKVYPRNNDYQTVYLKNVITRENIEVGDYTIYNDFCND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHTLKSKSTYTFPIFYEEW
                     GLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVHIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNNTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDELV"
     gene            complement(4638..5507)
                     /locus_tag="PC523_002027"
     CDS             complement(4638..5507)
                     /locus_tag="PC523_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2475550.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELIELRKLGVKMIYLGLESGSNEVLEDIKKGFNSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGKISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(5603..6460)
                     /locus_tag="PC523_002028"
     CDS             complement(5603..6460)
                     /locus_tag="PC523_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2475551.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKKNNRKFN
                     IKNKIFKGIGILFVLILLSFFIWINKTYEPQKLAKEALISNSKVEVTVKENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVVASNFARDNKLIDGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNLS"
     gene            complement(6675..8693)
                     /locus_tag="PC523_002029"
     CDS             complement(6675..8693)
                     /locus_tag="PC523_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2475552.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYIFSYDLK
                     RAVYVPFGLMYIFMVSIPVGISEFPMRLSALAAGAVIIMIAQFVMNRNRMKNIGDKEL
                     ISICDELLEKISLLKNIINDDSSISKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERINLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEVNYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKI
                     KSRDEKKAERKSNIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIDMYKGIVKNIIDEVNKSIENVADVYYIKNLLLVPSLIEDRIMLINAI
                     YKDKYQEDFLENQKLLISNMYNLYINVNKNKIQDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKTLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEVA"
     gene            complement(8965..9741)
                     /locus_tag="PC523_002030"
     CDS             complement(8965..9741)
                     /locus_tag="PC523_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF2475553.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALILGLIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     VLCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSIANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            complement(9798..10769)
                     /gene="asrC"
                     /locus_tag="PC523_002031"
     CDS             complement(9798..10769)
                     /gene="asrC"
                     /locus_tag="PC523_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HDF2475554.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLTIVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTKFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDGDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDENSIIKIILNTYKYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGVKYL"
     gene            complement(10782..11573)
                     /gene="asrB"
                     /locus_tag="PC523_002032"
     CDS             complement(10782..11573)
                     /gene="asrB"
                     /locus_tag="PC523_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HDF2475555.1"
                     /translation="MNPYIPVNAKILDIVKHTDKEWTFRVNCDTKGVLPGKFYEISIP
                     KYGESPISVSGYGENYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIAGFKSPKDVLFADDLKRWSKKLDI
                     LVTVDGADEGYSGNIGLVTKYIPELDIKNINNTSIVVVGPPMMMKFTVGEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDNTYICLDGPVFDYSYAKNLID"
     gene            complement(11574..12581)
                     /gene="asrA"
                     /locus_tag="PC523_002033"
     CDS             complement(11574..12581)
                     /gene="asrA"
                     /locus_tag="PC523_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HDF2475556.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVSIPFKGTFSDTDVIRYK
                     EVNSFEEMEFTKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIREKVKFVLVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEIFNGETTEFNIDYVTDNLISVDVPENIDSNEMIRNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVTSKEE
                     I"
     gene            complement(12972..13661)
                     /locus_tag="PC523_002034"
     CDS             complement(12972..13661)
                     /locus_tag="PC523_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2475557.1"
                     /translation="MSKLKDYNIDKVMIFNKLSTKTKNQLKEKAKLKRLQKKEILFYE
                     KDSLDSVYMLLEGKVSIFKISENGERKVIFILNSGEVINDITFDSSKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQSDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
     gene            complement(13724..14572)
                     /locus_tag="PC523_002035"
     CDS             complement(13724..14572)
                     /locus_tag="PC523_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2475558.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            complement(14591..15478)
                     /locus_tag="PC523_002036"
     CDS             complement(14591..15478)
                     /locus_tag="PC523_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2475559.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGLIIDKSSLDTEAKEKGGEGIL
                     SKIKGIVKKYIKENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEIEKEF
                     NIKCEVENDVNCAGLGEMWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNINGSSFQELASTSSLIKKVAKIKNLNENELNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            complement(15536..16339)
                     /locus_tag="PC523_002037"
     CDS             complement(15536..16339)
                     /locus_tag="PC523_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475560.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESVIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NAGCATLSKHYNIENKYVIWIQGESDAKYGSDALYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACNECDILCVGSKIAMQFHDKGLTIDDVHYTQE
                     GLNILGKDLCKNIYKYHTTKEKELLSDTIDLSEARRYIYELEKISKKFV"
     gene            complement(16499..16951)
                     /locus_tag="PC523_002038"
     CDS             complement(16499..16951)
                     /locus_tag="PC523_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDF2475561.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEEGDFL
                     PLDGKNNGYVELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            complement(17081..18583)
                     /locus_tag="PC523_002039"
     CDS             complement(17081..18583)
                     /locus_tag="PC523_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDF2475562.1"
                     /translation="MVGFTWIDFTILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIVVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YCMPEVTSWAYSLITITTSVVVGTIFSAIEAKVKAKVSLPRAETTVYYEK"
     gene            complement(18616..19482)
                     /locus_tag="PC523_002040"
     CDS             complement(18616..19482)
                     /locus_tag="PC523_002040"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDF2475563.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            complement(19567..20238)
                     /locus_tag="PC523_002041"
     CDS             complement(19567..20238)
                     /locus_tag="PC523_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDF2475564.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCISTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKRVFELGVYSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            complement(20514..21005)
                     /locus_tag="PC523_002042"
     CDS             complement(20514..21005)
                     /locus_tag="PC523_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2475565.1"
                     /translation="MEGNINNKVDDKFSKVKKKFGNGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSIDDNSFDKFCDELLELDKLIYEKNMEINEMEMVESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIVCGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            complement(21027..22391)
                     /locus_tag="PC523_002043"
     CDS             complement(21027..22391)
                     /locus_tag="PC523_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2475566.1"
                     /translation="MGEKKINNMNYSHNNYSKTTGEPTRKIKNSHITLKNKEEKICSS
                     CNKENNTKDNYCKFCGNELYEITSLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKSVLANSIGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIKTFIL
                     GYVLVLIILFVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIKPIVVFSIYY
                     AFLMGMLALFSTFILDSNINFFNTTSYGATLIMQFKVLQAIVISFIYSFVISLIGYKL
                     NSAD"
     gene            22810..23985
                     /locus_tag="PC523_002044"
     CDS             22810..23985
                     /locus_tag="PC523_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2475567.1"
                     /translation="MNLEKIKKFWGFPLDKKHKENVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFATPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKLGESVIVFGGCFLF
                     LNFFLPGIQTIPGNEFSNIVNTFFITSLAVFVSTMLYRSRVFTYYDKLLISQQYEEIY
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCDEEDAKLKSECLRKII
                     ETCNFERDDIPLKCVTVSIGVYTNKIERTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            complement(24158..25555)
                     /gene="asnS"
                     /locus_tag="PC523_002045"
     CDS             complement(24158..25555)
                     /gene="asnS"
                     /locus_tag="PC523_002045"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDF2475568.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFVRITYTKAVELLIESGHDFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     misc_binding    complement(25632..25865)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(26023..26217)
                     /locus_tag="PC523_002046"
     CDS             complement(26023..26217)
                     /locus_tag="PC523_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HDF2475569.1"
                     /translation="MENKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVTEAKDWVDNGSKL"
BASE COUNT         8965 a         4347 c         2638 g        10371 t
ORIGIN      
        1 aataaaaaat gctataatta acatattatc aattcgtttt attattgtag tacggctatg
       61 aaattataaa aatatttgtc taattaaaaa tatgatttat ccatatttgt ttattgtaga
      121 tacagactat acaaaaataa atagcactta tttcagtata aaaacatata tagtctatat
      181 ttaaactaaa aaagtataaa tagtctatat aaaacaactt aaactatgaa tataatctaa
      241 ggtggaggga taataattgg caaatatagg aaaaataata ggagatagaa taaaagtcta
      301 tagaacaaaa ttgggatata gtcaagagtt tctagcagaa aaggcaggtt tacatccaac
      361 atatattggt cagattgaaa gagctgagac aaatacatct atcaatatta taatgaaaat
      421 tgctactgct ttagacatac cacttgaatt gttatttgca aatattatta caacagaagg
      481 cgttgataac tctgtacctt tagaatgtta tgaaatgatt aataaactaa ctgaaaaaga
      541 acaaattgca atgctagagt taataaagtg catcattaaa tacaaagaaa tataagcgag
      601 ggaaatagta acaataatga attatatagg taaaagactt aaagaagaac ggaaaaaagc
      661 aaatttaact tcaaaagaat ttgcaaatat ggttggagta tcgccttggt atataactca
      721 aatcgaaagt ggtaaaaaga atccaagtct taaaacattc ataaaatttg taagtatatt
      781 aaatatctct gcagatgttc ttataaaaga tattacttcc actggaaaga catatttaga
      841 aaatgatata aatgaagagt taaaagactt aaattcaaga gaactaaatc tgatattaca
      901 gattatagac atcataaaaa agaataaaat ataattcttt atattttata taaaaaaaaa
      961 ggttatttga aataaataac ctttttttgt ctttttgtgt taacattgtc ttatagtgct
     1021 aatgagtact attagaccat gttctatggc cttattttaa tataaaacga ttggagatag
     1081 gctatgattg tgattgaagg tagcgataaa tttaaaattg caaaagaata tattgatgta
     1141 gaatatactc tttttagcaa agtaactttt aagtatgaaa agttaaaatt taaagataat
     1201 gctgaattag aaaaaattaa aatgtttaaa tataaaaatg gctacatccc taataaatta
     1261 aacctttctt taggatatgg attctcttct tataaaaaac aaataattag agaaactgta
     1321 gatactttaa gattgacaga aattttttca agcgagaaca tagaagatat taaatttata
     1381 aaagatggta caaaaaaatt agaaattagc atagagaaag ttgtgaaatt taaacgttga
     1441 aaaaaaagaa attatgtttg ttgctattgc cctgatatgt atagagacat aaaactcgac
     1501 aaagaatcta tcgataaaat atacaacaga agaaaaataa aaatagaaag agaagttaat
     1561 atttttgaag atgaggatgt tataataaac aaaaaagtat tgaaatttcc aaaatcttgg
     1621 acaaagaata tgcaaaaata ttggctaagt gaaaataagt atcccataca ttctactgta
     1681 attgatgatg atagatataa atgttgtaat gtacaatata caaagaatag agtgataata
     1741 ttatattaca tatataacct ttaatataca taattacaaa aaagtaatta caaatacaca
     1801 taatacaaat atatcttcta ctttagataa aacagttatt ataaaaactt actatctata
     1861 aataatagaa atgctagtat accatctata atccaatgtc tagtaggttt taatggcata
     1921 ttttaaatat gccatagttg atttataaga tttttatacc actttcaaaa tacacactaa
     1981 atagttgctt ttcagatatt acacttaatt aaccaattta aattttacta tctgaaaagt
     2041 tttttatatt taatttatat atcttcaaaa ataactccta atatttctat ataattatat
     2101 taaattaatt aatactctta tgtaataaat taagtactga gcttaactct ttaaatgata
     2161 tttgacctgg tgctgaaaca ctttttgcag ctccaaaagt taatgcagaa ccaaatattt
     2221 ccccacacaa tctgcttata actcccattc ccgacattga cattgttatt attggtctat
     2281 ctgcataaat tttaaacatt tcatttgtag cttcaagtag aactaacaca tctttttcat
     2341 cttgtggcat aacagctatt ttgggtaaat ctgcgcctaa ttcctgcatt ctacataaac
     2401 gagatacaat ttcttctttt tttggagttt tattaaagtc atgattagat ataattactt
     2461 tcacttcttt tttatgagca aaattaatta cttcatctat aacctcatct cccataaata
     2521 actctacatc tattaaatct actaaacctg tactagaaat ttctttattt aaagtagtat
     2581 aataatcttt tgatattact ttttctcccc cttctgctaa acttctaaat gtgaataata
     2641 aagggatatc agatatataa cttcttaatt catagagaac ttccttaact tctttaatat
     2701 tttctacatt ctcaaaaaaa tcaactcgcc actctataat atccaaacat actcctttta
     2761 attctttagc ctcttttata atatcttctt tatttttacc gataatcggt acacaaattt
     2821 taggtctacc ttctccaata gtaatatttt ttacttgaac ttttcttttc atattctcct
     2881 ccataatcag aaattctata tgattttaaa ttctattttc tgcataattt aattatacat
     2941 tataaatgct acacattata aaaatactaa tttaaaaata atttttattc taattcttaa
     3001 aacaatccca catattttag aaaagatact ctataatata aaaacacttc tttcttatct
     3061 aaatttattt taacattcta taattttcat aacattactt aagttttatc tatataatca
     3121 atattattct ggatgataat agaattaata tataaattat agtataatta tatagaaata
     3181 actcgaattt tactacttat gaagaaattt gatatcaata aattcaaatc atttgcatta
     3241 cttcagagtt ttggctaaag ttaaacacca tactaaaccc aaaatagatt ttacagctat
     3301 acttcaagag taattgattg taatagtacc atataatcat cctttagcat taagtgacag
     3361 tgtagattaa aaggataaca atgttaatgt tctttaaatt aaaaattatt ttgtatataa
     3421 aatatattaa attattgcaa gctttattgt tttgatatac aatcaactaa gcatatagcc
     3481 tttataaaat actaaatagt taaaagtctg tttttaaaaa caaaatgtat aaattaaaat
     3541 atagatacta tcaaggagtg attctgtgaa gagatgtatt gtaatagtgg aatagtcaaa
     3601 aacataatat atatcaaagg agagatttaa atatgactat tccaaattca actaaggtat
     3661 atccaagaaa taatgattat caaactgtat atcttaaaaa tgtaataaca agagaaaata
     3721 tagaagttgg agattacaca atatataatg acttttgtaa tgacccaaga gattttgaga
     3781 aaaacaacgt attatatcat taccctatta acaatgataa attaataatt ggtaagtttt
     3841 gctcaatagc atgcaatgca aaatttctaa tgactagtgg aaatcacaca ctgaaatcaa
     3901 aatccacata tacctttcct atcttttatg aagaatgggg actcgatatc aatcatataa
     3961 cggatgcttg ggacaataaa ggtaatatta taattggaaa tgatgtgtgg ataggctatg
     4021 attctataat tatgtcaggt gtacatattg gcgatggtgc aattattgga acaagagcag
     4081 ttgttactaa tgatgtccca ccatattcta ttgttggagg agtacctgca aagattataa
     4141 aaaaaagatt taataataat acaatttcaa aattattgga aattaaatgg tgggattggc
     4201 catataaaaa aatccaatca aacattaagc atattcaatc aggaaatata gatgaattag
     4261 tttaatcttt tataatttat atatattaaa ttttacttta ataatccttt ttataagaat
     4321 tttttcaaga tttgtatatc attttaatat atataaattt aatacaatac taaacaaaaa
     4381 taaaaagtaa agttgcttaa tataaagtta ctttactttt tattatttaa attcatgggt
     4441 ccattataat atgtaaaatt ctattaaacc agttcacatt ttcatcatta ttgatagcct
     4501 atatatgaac ataccatttt gttttttaac aaaaaaatca tgctatatta atagtaaaaa
     4561 actattaata tagcatgatt ttttgtatct aaacaatctc agtatttatc tataaatata
     4621 gattgatata tcaaaaacta cagtcttcta tattcttctc tttttaactt ttggttactt
     4681 agatagtaat caatttcatc aattagacgt tgtttgtcat caggtaatac accttttaaa
     4741 ttagcataat ttgatgcatg attacttcta aatacaattt tttcattggc atctatattc
     4801 tttatcatct ctttaatttc ttgtaacaca gccttatcct ctagtattgt aaactctcct
     4861 gccttaagta aatcgtaaag ttcagtatta ggttctacca ttaaactaag tacacctaca
     4921 taatctggtg aaatttttcc taacattttt cctgtatcaa cggcatgctg atgtgttttt
     4981 ttagttcctc caagacctgc tattacagtg gctgataatt taatcccact ttttttaact
     5041 tttctaccaa cttttattaa ttgttcacta ttaaaacctt tcttaatatc ttccaatact
     5101 tcattgcttc cactttcaag ccctaaataa atcattttaa caccaagttt tcttaactct
     5161 ataagctcat catcagattt tttctctata gatttaggag aaccatacat agtaattctt
     5221 tcacactctg gaaaaacttc ttttatatat aaaattatgt ctcttaattt ctccattggt
     5281 attatcaatg catctccatc tgctaagaat attctttcta catgtttata atatcttcta
     5341 aatatatcaa tttcattttt tattacttct agtggcttta ttttaaattt tttagatttg
     5401 tacatactac aaaacgtaca tttgttgtga gaacatccta gtgtgacttg tattataaga
     5461 ctataagcct cacttggcgg tctgtaaagt ggcatatcgt attccatatt aaaatccccc
     5521 ttgtaataaa atatttattc tattatatta ttaatattta ctttaaataa taacttataa
     5581 aattatattt tttaatttta gtttaagata aattctttaa aaatttaatg atactattgg
     5641 cagtaatatt aagttgtttt tcttgactta taatagcatc attatctccc ttttgttttc
     5701 catagtctcc aaattgtgca tgatttcctc cttcaatttc aacataagtt gtatcatcag
     5761 gaagtttttg ttttgattct acaagacttt taaaattgac tacaccatct ttgctccccc
     5821 atatagatat aacttttttt cctaattctt tcaactcatc tcccattgga taagaagcta
     5881 aaaatacaac tccatcaatt aatttattat cacgtgcaaa atttgatgct acaactcctc
     5941 ctaatgaatg acctcctatt acccaatgtt ctatgttgtt gtaagaatcc ataactttct
     6001 gcgccttatt agttccaagt atagctagat taagtggcat ctttacaatt acaacctcat
     6061 atccactttc tgctattttt cttgcaagtg gtgcataaga ttctatttct actttagctc
     6121 ctggatacaa aattaaacct ttagaaactt ttcttccttt tggtgtaaat gaaatgtttt
     6181 cttttacagt tacttcaact ttactattgc tgattagagc ttcttttgcc agtttttgag
     6241 gttcatatgt tttatttatc catatgaaaa aactaagcaa aatcaataca aatagtatac
     6301 ctattccctt aaatatctta ttctttatgt taaattttct attattcttt ttatcatctt
     6361 tgttttcaac ctcatcataa agtaaaggtc tttcgagttc catagttata ctaaatgtat
     6421 catatttctt attgcgtttt acctttacat ctttactcat aaatctaacc cccataatat
     6481 ttgaatacta ttatagttta tcctaagtaa attgtttttt caaccaatga aaaaaatcaa
     6541 tattttaact attttcatat aaaattcata aatatatgtt ttttgttttt tatatattat
     6601 tttcttttaa tattttataa aacaaatagc catatacttt attttaaata tatggctatt
     6661 ctataattta atatctaagc tacttctttt attgaaactt tagttatatt acttattcta
     6721 tacatttctt tgacaccatt tagagtttgc agtaagttta gacatattat cttgtctcct
     6781 agagttttag tatttacaat actctctaaa attacgcttc ttccctcatc atacttatct
     6841 acattgtaat ttgaaatata attagtatct ctcaatattt tttcaacatc ttcatcttgt
     6901 attttatttt tattaacatt tatatataga ttatacatat tacttattag taatttctga
     6961 ttttctaaaa aatcttcttg atatttatct ttatatatag catttatcaa cataattcta
     7021 tcttcaataa gtgatggtac caataataaa ttctttatat agtaaacatc agctacattt
     7081 tctatagact tattgacttc atcaattata tttttaacga ttcctttata catgtctatc
     7141 acatgttgat agcctgtttt agcatcatat ggtaatataa acttatttac tattaatgca
     7201 ataattgctc ctaatgctac atatgaaatc ctgcttattg ttaaaacatt tgggtctccc
     7261 ataactacag ctgaaccaag agctgaaaca gttacacata ccattaattt tctataatca
     7321 actacataag agtttatata tcctgctaaa agaactatta acgaccttaa tgtactatct
     7381 tttattattg aaaataatac aataaatatt acagctccta taaatgtacc ttctattctt
     7441 tttctagacc tttgtataca attttctgcg taaggttgtg ttaatgaaaa aacagttaac
     7501 attatccatc ttccatccct taaatggaaa taatccataa taaaacccgc tacagctgta
     7561 gctaaagcaa tctttactgc ataagaaaat cttatagaat ttaaattaaa gtttttctta
     7621 tatattgata gtcgtttaaa ttcatgaggt atatttgatt ttctttcagc tttcttttcg
     7681 tctcttgatt ttattttttt ataatctact aaaaactcat ataaattttc taaaacacta
     7741 taaatttctt ttaaatttat atttttagta tcctgagcaa taacataatc tctaaatcct
     7801 aatatctctt tatcagttat ggtatcttta ttttctaaac acatcttaat atagtttact
     7861 tcttttgttg ctacaactag aacatctgat ttactattta tgctactttc ttctaaaata
     7921 ttttttatat cttcatcttc aaattcttta ctatcctttt tgtatctttc taggattaaa
     7981 tttattcttt ctaatgaaaa taaaatattc attatgtcta tacctctaat acttatataa
     8041 aagtcatctt ttctattatc aaaaataacc attttcaaat ttttagatat agaatttatt
     8101 ctataattac agctatctat ttttctcata ctagatttac ttatagaact atcatcatta
     8161 ataatatttt ttaaaagact aattttttct aataattcat cacatataga tatcagttct
     8221 ttatctccaa tatttttcat cctatttctg ttcataacaa attgagcaat cattattata
     8281 actgctccag cagctaaagc cgacaatctc ataggaaatt cacttattcc tacaggtata
     8341 cttaccataa atatatacat taggccaaaa ggtacatata ctgctctttt taagtcataa
     8401 ctaaatatat accctattat aaataatgct ataaaattta ataaaacgcc taaatacata
     8461 ttttgtactg caaaaaatga tagtattcct aggattatat ttattgctaa atactttagt
     8521 aaatttttaa ttggagaaat agttaaatct ctttccaata gtgttagcat agctgttact
     8581 ataccaacac ctataagtgt gtttttactt ccaaataaat atatgaatat agaaataaat
     8641 gctattataa aaataaacaa ttttgttttt gatattacta gtttctttgt cattatatcc
     8701 cccctaaaaa tttcaacaag accaatttta tcatatttgt tatcttattc aaaatactct
     8761 tttttacggt aggactttaa ctttttttac attttgaact atataatcta tatttaaacg
     8821 tattttattg catatatagt ttatatattt tcgtatgctc ccatatattt tttctttcaa
     8881 atattttttc aaaaatataa aaataactct ctcaacaata ctaaaactgt atttattaag
     8941 agagttataa tttatacaat aaatttaatt aagcttttct ttacccaata tataagttcc
     9001 aagacccata aataaagctc ctccaacaat atttccaagt gtcacagcta ctaaattata
     9061 aatagctcct ccaatagtaa ctgtacttat tgttggtgaa aataatgaaa cactataaat
     9121 tgtcatattt gctatactat gctcaaaacc tgatgttata aaggcaaata aacataagaa
     9181 tatcataatt atttttgcag tatcactatt agttctaaac gaacacaata ctgatacaca
     9241 taccagaata ttacataaaa cccctctaaa gaaaagagct gtaaatggca tggatgcttt
     9301 actagctgct gtatttgcaa aaaattcagc tacaggtcct ttatctacta gaccagttcc
     9361 tacaaatata agacctaaaa ttaaagctcc aattaaatta cctacccaac tgtatgccca
     9421 tattttagaa gtatctttta tacttacacc tttatttaac ataccagcag acataaccat
     9481 attgtttcca gtaaataact ctgttcctgt cattataact aaacttagtg ctattgcaaa
     9541 ggataacccc attactatct tagtcatagg tgaacctgca tcagtcaaaa gtcctccaat
     9601 agtaaatatc aaaagtatac ctattcctac ataaagtcct gcaaacgcag aagataccaa
     9661 atattttact ttacttgtgt ttaataaatt tatcttattt atagctgcat tagttaattt
     9721 atccaaagtt tctttatgca taattccctc ttccttctat taaattttaa tttattcaaa
     9781 tatatgtttt actcaattta tagatattta actccactcc agtatatgtt ttcatacatt
     9841 ttagttattt ctggaagttc tacaccttca agcgcccatt ttttaaactc cataaatcct
     9901 gttctatcaa ttatatatcc tatatgttct tttcctcctg gtgcatcttt atctatatac
     9961 ttttctacat atttgtaagt attaagtatt atctttatta tgctattctc atctacccat
    10021 aaaagaaaat cttctgctag tctagggttt ttctttcctg ttctacccat tatagctaat
    10081 ctatagtatt ttttctcatc tctagtccat gcatttgttg ggcaatttag tacacattct
    10141 ccacatccta tacatttatc tccatctctt accaccttat tattttctgt ttttaatgct
    10201 ccaacagata ttttagaaca tttttttaca cacgctccac aatttacaca tctatctttt
    10261 tcatatatgg gtaaagccat accaataatt ccaaaatcat tcattctagc ttttatacaa
    10321 tcatttggac agccagttaa agccacttta aaatgtaaat catttggaaa tatagctttt
    10381 tctattcttt tagcaaattt agttgtatta tattgagcct ttggacagac tttatttcct
    10441 atacatgctg ctatatttct tgtacccgct gctggataac ctgcattttt ctcactttga
    10501 tttatattca ttttttcaat aacaggttgt ataattttat ttatttcttc catatcttcc
    10561 atatttattc ctaatatttc aaagccctgt cttgtagtta tgtgtacctg tccattacca
    10621 tactcacttg caattttaga aactatagtt aaactctcag ggtcaataag cccaccaggt
    10681 actctaaccc ttaaagaagt ctcatatttt gtttttgtaa tcctataagc atttttcatt
    10741 acttttttgg tatttaaatc tcttatcaca aacttcgccc cctaatcaat aagattttta
    10801 gcatatgaat aatcaaatac tggtccatct aaacatatat aagtgttatc cattttacag
    10861 tgtccacatt tacctatacc acaacacatt tttctttcat aagaaaccca aatattcttt
    10921 tcatctaaat ctctttttaa aaattctcct actgtaaact tcatcatcat tggaggtcca
    10981 actacaacta tagaagtatt atttatattt tttatatcca attctggtat gtattttgtc
    11041 actaatccta tatttccact atatccttcg tctgctccat ctacagtaac taaaatatca
    11101 agttttttac tccatctctt caaatcatct gcaaataaaa catcttttgg tgatttaaat
    11161 cctgctatta atttaaactt ttcgcatttt tctttattgt tgtaaaaata ttcaactatt
    11221 cctcttacag gggctaatgc acttcctcct gccactacta caatttctct tccttcgtaa
    11281 agagatacat caaatccatt tccataaggc cctcttataa aaaaagaatc tccttcttta
    11341 taattaaaca actcactagt tacttttcct acatttctta ttgtaaagtc aatatagttc
    11401 tctccatatc ctgatactga tattgggctt tcaccgtatt taggtataga tatctcataa
    11461 aattttccag gcaatacacc ttttgtatcg caatttactc taaaagtcca ttctttatca
    11521 gtatgcttaa ctatatctaa tatttttgcg tttactggta tatatgggtt catttatatt
    11581 tcctccttac tagttactaa atcatttatt ttttctatac attttgaaaa tgatatgtat
    11641 tgtggacagg catcatcaca tcttccacaa ccaacacaca tatgatatcc gaatcttttt
    11701 ttaaaatcat gtattttatg catagtttta aatctcattc tttcaccttg tttttttcta
    11761 aaagaatttc cacctgctat atctgtatat ccatctactt gacaagaagc ccaaactctt
    11821 cttctctcac ctacattctc attttcctta tagaaaatat cctgcattgt aaaacatgaa
    11881 catgttggac atacaaaatt acatctacca catcctatac atcttgtatc atattcatcc
    11941 catatagaat ttcttatcat ttcatttgaa tcaatatttt ctggcacatc tacagatatt
    12001 aaattatcag ttacataatc aatattaaat tcagtcgttt ctccattaaa tatttcaaat
    12061 tcactatcct ttatgtctaa atacagtgta tctccttcta tatttaatcc aattgaataa
    12121 ttatcagttt tgttagaatc catactcaca caaaaacagt ttctaaaact ttccttacac
    12181 cctacaagta caaactttac tttctctctg atatttttat aaaaataatc tttttcttcg
    12241 ccattattta gatatatttc atcaagtctt tttatcccat ttatgtcaca agctctcaag
    12301 aaaattaaaa ttttcttgtt gtctaaatcg ctttccttaa actctttttc agtaaaataa
    12361 aataatactt gatttatagg aagtacaact tcttttggtg agaaatttgc tttcttggta
    12421 aattccattt cttcaaaaga gtttacctcc ttgtatctta taacatcagt atctgaaaaa
    12481 gtacctttaa atggtattga aactggtgca aatatcttgt agtctttttt tagataattc
    12541 agaccttcat tgaatttaga tgaatctaat tttaatttca ttcttatcac ctttcttata
    12601 ttttagtttt acttacttct tatcttgttt aaatcttaac attttatttt ctatgcttct
    12661 gtgataatta tcacagtttc aaaaataata aaaaaatatt aatagagttt attaagctct
    12721 attaatattt ttttattatc tatattattc gattacaaat ctagtcactt aaatacaatc
    12781 attaaataca ataaatctat tatctactca actttttgaa taatagagag tcttatttgc
    12841 ttttttatac aatttaggaa aggtaaaata aggattaata aattagacaa taaaaaagaa
    12901 tctataaata gattctccca ataatatttt taattaatta aaatcctaat aataccctta
    12961 tttaatcata actaattatt tttaaaatac cttgataatt cagtttcttt aacgtaaaaa
    13021 cctttattat caatcttaat aagttcttct ttttgtaata cctttatcat tctagaaaca
    13081 gtctctcttt tacagccaag catatcagct aaatatgtaa ctgtaatatt tacatttagt
    13141 aaagtccatt ctccacagca aactccaaat tctctactta acctatataa ttttgctgcg
    13201 agttttttat ctacttttat agatatagaa tttttcaact gtctatataa acgtcttatt
    13261 cttctttcca tataagaaat aatatctttt gtaagttgaa aatcactttg cataatttct
    13321 aaaaactctt caatactata ataagctaca attgagtttt caaatgcttc acagccaaca
    13381 gtggagtttt tagaagagtc aaatgtaata tcatttatga cttctccact atttaatatg
    13441 aaaattactt ttctctcacc attttcactt atcttaaaaa tagatacctt tccttctaaa
    13501 agcatataaa cactgtctaa gctatctttt tcataaaata aaatttcttt tttttgtaat
    13561 ctttttagtt tagctttttc tttaagttga ttcttcgttt tggtacttaa cttattaaaa
    13621 atcattactt tatcaatatt ataatctttt aatttagaca tgtaatatct ctccattcct
    13681 aagtttttaa gagagctcta taaaacatta caaatttctt attttattca tttttaaaca
    13741 attttatagc atttgtagtt ttaatttttc tttcaatagc ttcattagaa agttctttta
    13801 ctacttgtgt atatactaaa tcaataatga agaattgtgc taatttagaa gatattgaac
    13861 cggtttccag tactgtttct cctgatacat aagataagtg tacatctgaa atatccttta
    13921 attcagaatt tttattttca gtaattgata taattttagc attattggct cttgcaagct
    13981 ttacagtttt tataatctcc tctgtttcac cagaatgtga aattgcaatt attaaatctc
    14041 cttcttccat tatggaagac atcattatca tggtatgact actgtcaaaa cttacacaat
    14101 taagacctat tctcataaat ttatagtttg aatcctgtgc tattatacca gaatatccaa
    14161 tacctatgaa gtaaactttc tttgcattta ttataagcct tgcacagtca tgtacatcct
    14221 tactattgat tatctccact gtgttctcta aagtggttac atttgaactt aaaagttttt
    14281 ttgctgtatc taaagctggc tcatcatttt gtatattttt atttataata ttttttttat
    14341 tgatagttga tatttcttgt gctaaagtaa ctttaaaatc ctgtaatcca ttaaaattca
    14401 ttttttttac aaatctagtt atggttgctt caccaatatt gctttcttta gctatttgtg
    14461 atattggttt ataaaacaca tcatctatat tttcttttat ataagctatt aataatttat
    14521 ctgactttgt gactttaaaa gttggtgatt ccaattgttc taaaatactc attaaaaacc
    14581 cttctattct ttatattttg tttaaaaaat tatataatgc cccaagcatt cccgcatcat
    14641 tttgtctctt tgcaaattct atattagtgt ttttgtaaac tctctctata agaacctcct
    14701 ttaatgcttt gtctattttt ggttttagga acttttcttg tgccataatc ccaccaccta
    14761 aaattactac ctctggattt attacataac atatatttgc gattccaact gctaatgatt
    14821 ttatcatatt atcgagttct tttaaacaat cttcgtcatt attctttgct aaatcaaaaa
    14881 ttatttttcc atttaattca ttttcattca aattttttat ttttgctact tttttaatta
    14941 gactgctagt agatgccaat tcttgaaaag aacttccatt tatattcata tatcctactt
    15001 cgccagcact attactaaac ccatttatca gtttattgtt tattataata catccaccaa
    15061 ttccagtacc tattgttaaa catatagaag atgttgcacc tctacctgct ccaagccaca
    15121 tttcaccaag tccagcacaa ttaacatcat tttcaacttc acattttata ttaaactctt
    15181 tttctatttc tttttttaat tgcataccct tatagtttgg aattaattct tctaatgcaa
    15241 atagtatttt accttcaact gggtcaacca tacctgcagt tgatatgcat ataccactta
    15301 tttcattttc ttttatatac ttttttacta taccttttat tttagataat attccttctc
    15361 cacctttttc ttttgcttca gtatcaagac ttgatttatc aattattaat ccatcttctc
    15421 taataaatcc atattttatt gaagtacctc caacatctat acaaacgtaa tttttcataa
    15481 catatctcca tataaattat ttttataaat tgataacact acttaaactc ttatattaaa
    15541 caaatttttt acttatcttt tccaattcat atatatacct tcttgcttct gataaatcta
    15601 ttgtatcgct taataattct ttttccttag ttgtatgata tttataaata tttttacaca
    15661 agtcttttcc taatatatta agaccttctt gagtgtaatg tacatcatca atagttaacc
    15721 ctttatcatg aaattgcata gcaattttgc ttcctacaca taatatatca cattcattac
    15781 aagcagactc ctgtgctgct gcaattcgtc ttgttctaat aaaatattca tcttctccaa
    15841 gccaataacc agttaaactt acaaacattt tatcaatcat acattcttct ttaagtctat
    15901 aagcaatctt ttttagactt tctttatagt acaatgcatc actaccatat ttcgcatcac
    15961 tttcaccttg aatccaaata acatatttat tttctatatt ataatgttta cttaaggtag
    16021 cacagcctgc attaaatttt tctatagtgt catttaggaa ccagttatta tcatgattcc
    16081 agttttttat tcttgaacca tcttttgcat tgtgaataaa acaaacttta tttccagtaa
    16141 gcttattcca ttcatttgaa aatgcaggtg ctatagtacc atcaccttct gaagtttcta
    16201 gctcacatct catatttata atttcatcag tacttaaata ttcaaaacaa ttttcatttg
    16261 gaatgacact ctctttggga ttccctaatc ctctagcatt gctttgtcct attaataaga
    16321 atacatctat attttccata agttaactcc tttacaaaat tttaaacttt taattatttt
    16381 caatttcctt ataaaaaact aaggctgaaa atagtaatct aactaaaatt tacgaagtct
    16441 cactaatcgc tgaaagttaa atcactattt tcagccctag tggttatata aattataatt
    16501 atctaaccaa aaccttaaaa atagcttttt tgatattttc tttttctaaa acttttattc
    16561 ctgtcatatg agcatcttct ggataacaaa ctaaaaaatc gccttttcta agttctacat
    16621 atccattatt tttaccatca agaggtaaaa aatctccctc ttcttcatat attttttgtt
    16681 ctaaattttc tataaaattt atatctattc gttcacatcc atcaagcatc aaatgaacat
    16741 ctatatactt cttatgagct tcccagaatc tatcttcttt ttcacaagtt ttatactcta
    16801 caatattcac aaaaatatca tcaccatcta ttgtataagt acctttttca taatctatta
    16861 atgaattatc tttagcatat tcaaaacatc taagtaaatc ttcatgcaat gttgaatatg
    16921 tcttactatg atttatattt ccaaatatca taagcactat ctccctatat aattataaag
    16981 tctattttta tacttttata tataaagtat attatacaat cttatagagt aactaaatta
    17041 tttatctaat cactctataa gacaatttat catttaaata ttatttttca taataaacag
    17101 tagtctcagc tcttggtaaa gatacttttg ctttaacttt agcttctatt gcactaaata
    17161 ttgtacctac cactacagaa gtagttattg ttattaaaga atatgcccaa gaagttactt
    17221 ctggcataca gtattttaaa taaacaacca aaattgctga tactataaac ccaacatatg
    17281 ctcccatctt agttgctttt ttacaaaatg caccaagtac aaatattcct gctaagacac
    17341 ctagtactag acccataaaa ctattgaacc attcatatgc tgactttatt tctccatttg
    17401 ctaatactat tgcaacaact atagaaagta tacctacacc taatgatata tattgtgcta
    17461 tcttagtttg tttttccata cttatatctt ttgttatgat agtttgtata tctaaagtcc
    17521 aacttgtagc aactgagtta agccctgttg ataaagttga ttgagaagct gcatatattg
    17581 ctgcaagtag tattcctgtt atacctactg gtaactcata agctatatat gatgcaaata
    17641 cttggtcttg ttgaactgtt tgagctaaat ctggattttg agtatagtat ataaataaac
    17701 aagttccaat taaataaaat actgttgcaa gtcccattga taaaacacca tttccatatg
    17761 tcattttatt aagttgcttt atatcagttg ttgtagtaaa cctttgaact atatcttgac
    17821 tcgatacata tgatgaaaaa gtattaagtc cagctccaac aaatattata aatgcacttg
    17881 tagatagtat atttttatca aacataactt cattacttgc taagaacttg tgtccagttg
    17941 taagagtatc cattactgtt ccaaaaccac catttatact tgctattaaa tagaataatg
    18001 ttcctgtaac acctactatt agtaccattc cttgtataaa atcggtccat aaaacagctt
    18061 ttaatccacc tgtatatgag taaattattg ctattacacc cataactatt atcagtacat
    18121 ttacaggtat tcctgtaaga ccagccagaa ctattgatgg aagatacatt ataatagaca
    18181 tacgtcctat ttgatatatt ataaacatta aagctccaag cactctaaga cctttgcttt
    18241 cgtacctcat ttctaagtac tcataagctg tatcaatttc taacctacta tatagaggta
    18301 agaaaaattt tattgttaaa ggtatagcta taaacatacc taattgtgca aaccacaaaa
    18361 tccaagtccc accataagaa tttcctgcta atgataaaaa cgatattgga cttaataatg
    18421 tggcaaatat tgatactgat gtaacccacc acggaatagt tccatcacct ttgaagaact
    18481 cttttccctt catttccttt tttgaaaata acaatcctgc aaataaaact gccaataagt
    18541 aaaccactag tattgtaaaa tctatccaag taaatcctac catttgacta atcctcccat
    18601 actaaaagta ttttattaaa aatactttct gtatatttct tttgctcttg atttcatttc
    18661 atcagttgct tctttcatag gttgtctaca gtatccagcc tcaacacctt gttcttcaag
    18721 tagcaattta attgtttgat ataatccatt tcctaatata tctgttatta aatcattagt
    18781 cacatgttgt acttcaagtg cttctgatat tttttcattc ttagttaatt caaatatttg
    18841 tctagctctg acaccgttta cattaaatgt agaaccaatt gccccatcta cacctaaaac
    18901 tgtagctggt aacatcattt catcaaatcc tgcaaatatt aatttatttg ggaatgtttt
    18961 tctcattctc tctaataagt agaaatctgc tgctgtaaac ttaacaccta ttattttttc
    19021 attttcaaat aattctccaa actggtctaa actcatatca actccagtta aaaatggtat
    19081 agagtatatt attaatctat tatctacaga atttattatt gtattataat agtgttttat
    19141 ttcttcaaaa tcaaacttat agtaaaaagg tgtaacagca cttattgcat catatcctaa
    19201 atcagttgta aatttagcaa gctctacagc ttcttttaaa ttgactgaac caacttgagc
    19261 tattaatttt atttcttcct tcacttcatc tttagctatt tcaaatattc ttttcttttc
    19321 atcagttgaa agcataaagt tttcccctgt acttcctcct acataaagac catctacttt
    19381 acaaacatct atattatgtc taattatttg tcttaaccct ttttcattta tatttccttc
    19441 cttatcaaat gaaactaata atgctgaata aattcccttc atatctgttg ttctcatatc
    19501 tttacctcca attttaattt tcaagtatag ttaaatataa tagtttttta atttataaat
    19561 taatttttaa ttaatttcaa ttgctttaac aaatttttct gttattaatt gtggtcttgt
    19621 tattgctgaa cctacaactg atgaatagac tcctagttca aaaacccttt ttaaatcttg
    19681 aggagtattt acctttcctt ctgctattac aggtatttct aaatctttaa ctagtctttc
    19741 cattaataca aaatctggac cttctaacgt tttagtatat ggagtatatc cagatagtgt
    19801 agttgaaata caatctacac catattcttg tgctttgata gcctcatcat aatttgaaat
    19861 atctgccatt accagaacac cattttcttt tatatacttt ataagttctt ttaagtcttc
    19921 attatttggt ctaactctat tagtagcatc taaagctatc atttcacacc ctgttgtcaa
    19981 aagttcatca acttcttttt tagttggtgt tatatatata tctgaatctt cataatttct
    20041 ctttataata cctataacag gaagtccagt aacttttttt atctcaataa tatcttcaac
    20101 tccttgagcc ctaattccaa cagctccacc ttccatagca gccttagcca ttctccccat
    20161 tataaaagga ctatgaagag gttcattttc taaagcttgg catgatacta ttaatcttcc
    20221 ctttacctta tctagcattt tttcaattac ctcatttctg aaatttattt tcttgattta
    20281 tgtattaata ataacatgaa aactttttct ttgcaatatt ttttcatatt tttttcttaa
    20341 ctctatattt ttactgattt attctatata ttagtctgtt ttttatactt aaaagaaatt
    20401 ttatttcatt tttttggtgt ttattttcat tttgtataac cactattata tgtattctat
    20461 ataaataatt tcaataaaaa aacccatgta tactatacat aggtttccca atattattct
    20521 aaatccatta taatttttct tccacaacat ggacaaaatt cagcctctgt atctatctca
    20581 ctttcacaat aaccacaaac tataaaatca tcacattcat tttccaattc aaattttttg
    20641 ccacattcag cacaatattt atcatttaca tttccaacat agccacattc acaagttaca
    20701 ttactttcta ccatttccat ttcattaatt tccatatttt tttcataaat taacttatca
    20761 agttctagta actcatcaca gaatttatca aaactattat catcaataga atcttctcta
    20821 attttttgat atgtcaatat tcccatttcc aagaatatct ttgctttctt ttcttggata
    20881 tcatcaatct ctctatttaa ctttgatttt tctttatata attcaacttt aaattttcca
    20941 ttaccaaact tcttcttaac cttagaaaat ttatcatcta ctttattatt aatatttcct
    21001 tccacagatg ttccctccaa tcatatttaa tcagctgagt ttagcttata acctattaaa
    21061 gatatcacaa aactatatat aaatgaaatt acaatagctt gtaagacttt aaattgcatt
    21121 attaaagttg ctccataact agttgtatta aagaaattaa tattactatc tagtataaat
    21181 gtactaaata aagccaacat ccccattaaa aaagcataat atatactaaa tactactata
    21241 ggttttatac tatccgtatt atacttaaat cttagcttgt atccatttaa taataatatt
    21301 aacatagata atgctcccat tgcataaaac attaatttag tatctccaaa taaagatgaa
    21361 gaactcaaag taaacattga tactgttgaa tttattatgg ttactgaaat tccatttgca
    21421 aaagcccaca tatagctagc tatttgtggc agtatagtaa ataaatttaa tccatttgag
    21481 taactactca tatctagttc atttaagtaa ctactatcag atatagttag tacaaatagt
    21541 attattaata ctaaaacata acctaatata aatgttttta tagaatttga aaagagagat
    21601 aaatacatat tttccttttc ataacttttt ttataagtag ttatgtatgt acatataaac
    21661 cctaatacaa atccatttaa aaggacacta aaaaattcat aactatattc taatgcatat
    21721 ccatattcta acatactatg tgaactagtt tttacattag taaaaataga taaaatagct
    21781 aaaactaatc cataaaaaat tccaacacct atagaatttg ctaatacaga ttttgaatct
    21841 ctacatcttt ttctcataaa aattaagttt gatattaata ataccaaaat aggaattata
    21901 agtaatatta acaaacctat attggcattt ataaacccag aacccatcat ggtagacata
    21961 gatacactaa tttgacctaa gttgagagct aggattatat gtgctggatt tattaaataa
    22021 ctaatatcat taaattgaat agttataata gctttaaata tcaatgcaat aataaataat
    22081 ataaatatgg ttgtaaatgt tgttaaaaat actcctcttt tatttgcatg atatgaaagc
    22141 tcttttattt tagattttaa gtctagtttt gtttcaagtg gtcttaatga tgttatttca
    22201 taaagttcat ttccacaaaa tttacagtaa ttatccttag tattattttc tttattgcaa
    22261 ctagaacaaa ttttttcttc tttattttta agagtgatat ggctattttt tatttttcta
    22321 gttggttcac ctgtcgtttt ggaataatta ttatgtgaat aattcatgtt atttattttc
    22381 ttttcaccca tttttcccct ccaagtttta ctattgtaaa ttattaaata caactatatc
    22441 atataaaact taattatacc ttaattggat atatttatct attttatttg tttattgctg
    22501 ttgtttttta acaggtcttg atatttaaat atcttgttaa tgctaacttt attatttaca
    22561 ttttattatg cttaatttaa atttatttta ttctttattt tcagtagcaa actatcttta
    22621 aatcttaatt ataataaagt attaaccaat aaagttattc aacattagct aataaatttt
    22681 atccattaca attttagatt gttacacaca atatattttg atataatcaa attataaaca
    22741 aagtattcca agtatctaaa gacataaagg aatacttatt tttatgcttt atttttaatg
    22801 gaggcttata tgaacttaga aaaaattaaa aaattttggg gatttcctct agataaaaaa
    22861 cataaagaaa atgttgaaaa agaactttgc aattatcttt accatactgg taaaatcatt
    22921 tgcataataa tagtaatatc tcaaataatt atgatgatat caatctccgc tagagaggga
    22981 ggtccttttg ccacaccaag acgccaatgg tatttttata tgtatgtaac actattttgt
    23041 gtatcttctg tctttatgtt tgtattttct tatctttgga ataaaaaaaa ggattgttgt
    23101 actttttata taaatttatg gataatttat gtaacttttt tctgcctatg gggttgtgta
    23161 attactctac ttgaccagtt aggtggaaat gacctaagtg tatttagtta cattatgcta
    23221 gcagctgcag ctttctcagt attaaaatta ggagaatcag taatagtttt tggaggatgt
    23281 tttttgtttc ttaatttttt cttacctggt attcaaacta taccaggcaa tgagtttagt
    23341 aatatcgtca acacattttt tataacatcc cttgccgttt ttgtttctac aatgctatat
    23401 agaagtcgtg tatttactta ctatgacaaa cttttaatta gtcaacaata tgaagaaata
    23461 tatcatataa ataatttact taacgacaaa gtcatgacag atgaccttac taaaatgaat
    23521 aatcgtagat accttgaaga tattctacaa aaaaaattaa tcagccatgc aaatcaagaa
    23581 cctataactg gaatgatgat agatatagat tatttcaaac aatttaatga caattatgga
    23641 catcaaaagg gagactattg tttacaaaat attgccaaag taattttgga ttttacaagt
    23701 tctgaaaatg aacatgctat acgatatggt ggtgaggaat tttttatatg tatttttaac
    23761 tgtgatgagg aagatgctaa acttaaatca gaatgtttac gaaaaattat agagacctgt
    23821 aattttgagc gtgacgatat ccctttaaaa tgtgtaactg ttagtatcgg agtctacaca
    23881 aataaaattg aaaggacaat aacgctaaat gagtttataa gtaattgtga taaggctcta
    23941 tatactgcaa aagaacaggg acgtaattgt gttgcatttt attaaaaagt tacatactat
    24001 tacataaacc tataagtatt atattgcaaa ataaaagtaa ccaaattata tatttatagt
    24061 acaataaaaa taacttgcat atctctaaaa tagataaata tgcaagtcat tttagtgttt
    24121 acacttaaaa ttatataagt tttattatat ataattatta aaattcagca tttttaggag
    24181 ttcttgggaa aggtataacg tctcttatgt tagacatacc tgtcatatac ataattattc
    24241 tttcaaatcc aagaccaaat ccagagtggg tagcagtacc aaattttcta agttctaaat
    24301 accaccaata atcttcttct cttaatccca tttcattgat tctatctagt aatacatctt
    24361 ctctttcttc tctttgagaa cctcctacga tttctccaac tccaggaact aataagtcca
    24421 ttgctctaac agttttacca tcttcattca ttctcatata gaaagctttt atatcttttg
    24481 gataatctgt aacaaacact ggacaattat atatttgctc cgttatatat ctttcatgtt
    24541 cagtttgaag gtcacatccc cactcaacag gatactcaaa gtcatgacca gattcaatta
    24601 ataattccac tgctttagtg tatgttattc taacaaactc agagtttact atcttattta
    24661 atctttctaa caatccttta tctataaaac tattaaaaaa ttccatttct tcaggagcat
    24721 tttctaaaac atagcttata acatatttta tcatatcttc tgcaagttcc atgttatctt
    24781 caaggtctgc aaaaactatt tctggctcta tcatccaaaa ttctgaagca tgtcttcctg
    24841 tataagagtt ttcagctcta aatgtaggtc caaaagtata aacatttcta aaagaaagtg
    24901 ccattatttc ggcatttaat tgaccactaa ctgttaaatt agcctctttt ccaaagaaat
    24961 cttgagtata gtctattttg ccatcttctg tttttggaat atcatttaaa tccatagttg
    25021 ttattctaaa catttctcct gcaccttcac agtcactacc tgttatgatt ggagaatgtg
    25081 catatacaaa gtttctctct tggaaaaatt tgtgtattgc atatgcagct attgatctta
    25141 ctctaaatac agctgaaaaa gtattacttc ttggtcttaa atgtgctatc gttcttaaat
    25201 attcaagtgt atgtctcttc ttttgtagcg gatatgaact atctgattcg ccttctacaa
    25261 ctatgctttt tgcatgtatt tctactggct gtttagctcc ttctgtagat actaattctc
    25321 cctctacaag tatagatgag cttattgcta attttcctac ctctttaaaa ttttctactt
    25381 tctcatcaaa tactatctgc atatttttaa agaagcttcc atcatttagc tctataaatc
    25441 caaaattttt agatactcta gacgttctta tccatccagc tacttttaca gtcttcccta
    25501 tgtactgttc tttttctcta tacaattctt ttactgttat aaattccttt tgcattttaa
    25561 atcctcctag tataattgtt attaatttct tttaaatata gtttcaataa aaaaagccct
    25621 tcatccctat ataaagggac gaaagactat tactctcgcg gtaccaccct agttgtcata
    25681 atgacctctt agttaagcaa tgcttaaagc ctataacggt gccttccgtc taagtctact
    25741 tactaaataa gctttcgatt agaaactcca agatgttctt caatataaac ctcgtattga
    25801 gctttcacca tcctcaactc gctagaacta cattttatat ctactcttct cttcatagta
    25861 tttatctttt attatttctg ctaattaaca aaattatacc aatttataaa tttttagtca
    25921 ataaatttat ataattgata ctgattgcta tttaaaatat aataactcta ccttgcaaaa
    25981 tgacaataaa cacttatctt tactacttca gtttcaaaac ttttataatt tacttccatt
    26041 atcaacccag tcttttgctt ctgtaacacc cttaattgaa ggtattgaaa ctttactatg
    26101 aggttttaat ttttcaacat cagcccatgc tgctgtcgac tcatttccaa ctaaaagtga
    26161 acttttaagt cttttattat ctttaccttt taatgctctt gcttttttat tttccaaaac
    26221 tatcacctct attatttatt ttgtaatagt tttagcaatt tttcaaataa aattcataag
    26281 ttaaaattat aatgattttt aagtcaataa catatctttt t
//
LOCUS       DAMJVM010000045        26074 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.046, whole genome shotgun sequence.
ACCESSION   DAMJVM010000045 DAMJVM010000000
VERSION     DAMJVM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26074
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.046"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            287..1576
                     /locus_tag="PC523_002047"
     CDS             287..1576
                     /locus_tag="PC523_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDF2475570.1"
                     /translation="MRELLNVTLIIILVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIISIFNLSESIVYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLVVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRILVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
     gene            1605..2285
                     /locus_tag="PC523_002048"
     CDS             1605..2285
                     /locus_tag="PC523_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDF2475571.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWIETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTNIYDSSVPIIDLYDEKTKNMIEFMDREGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PQNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     SSKECHNL"
     gene            2386..2925
                     /locus_tag="PC523_002049"
     CDS             2386..2925
                     /locus_tag="PC523_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HDF2475572.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKEGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFTLLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSIYWTILTFIGVRYFQVYIVDSLYSLIIPYWPILVIILLATLF
                     VDEVLTIKFLRGNKDIVER"
     gene            3101..4120
                     /locus_tag="PC523_002050"
     CDS             3101..4120
                     /locus_tag="PC523_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2475573.1"
                     /translation="MMGKVRFGIVGTSNIANIFLKAASKVKDFELVAIYSRNLEKARE
                     FGMSHGASIFFDDLEQMAKSKDIDAVYIASPNALHSRQAIICLKHKKHVLCEKSLASN
                     LKEVKLMIKTAKDNNVLLMEAMRITCVPNFRAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKSVKAVSHVLQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSYENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            4410..5600
                     /gene="dltD"
                     /locus_tag="PC523_002051"
     CDS             4410..5600
                     /gene="dltD"
                     /locus_tag="PC523_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDF2475574.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTLLEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKSPINWSHERKKAIEDAKKRVGKNPLNIDNYYYK
                     QHFKDGIDQYKGRDKDVNLLTSKEFESYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQNIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            5721..7235
                     /locus_tag="PC523_002052"
     CDS             5721..7235
                     /locus_tag="PC523_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HDF2475575.1"
                     /translation="MKIIEGIKKYSNTDRIALICNGDKLSYKDLNEYSDAISIFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDEKDFGDINVIDMDKLKDIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPKLESMIFIGEALSKNLT
                     KELMSRFPNTRVINGYGPTEATVGVSVNDMTQKAIEDEKSLPVGYPMSNCKIKILDEA
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGIKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNVVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            7235..8383
                     /gene="dltB"
                     /locus_tag="PC523_002053"
     CDS             7235..8383
                     /gene="dltB"
                     /locus_tag="PC523_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDF2475576.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYVYWSFLFISMLPIIINKISP
                     VTSFGMIGFIGISYLNFRAIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSIIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKEKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            8402..8632
                     /gene="dltC"
                     /locus_tag="PC523_002054"
     CDS             8402..8632
                     /gene="dltC"
                     /locus_tag="PC523_002054"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDF2475577.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            8847..9632
                     /locus_tag="PC523_002055"
     CDS             8847..9632
                     /locus_tag="PC523_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2475578.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNSTNLDKKQSIAKYASKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASLYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            9922..11832
                     /gene="phnW"
                     /locus_tag="PC523_002056"
     CDS             9922..11832
                     /gene="phnW"
                     /locus_tag="PC523_002056"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDF2475579.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNLWSDKFGKVPIEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVIETIEKLRENGLKIGSTTGYTREMMDIVQPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTE
                     EEVENMDKEELKIKMNTVSKKFKEAGAHFVIETMAELEDVLVKIENKTIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNDLVQDVRRRLISLGTKNTDKYTSVLMQ
                     GSGTFSVEATIGSTIPENGKLLVIANGAYGKRMKDICNYLNIEFVDCTFKDVEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKVYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEELSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKANGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIERFINR"
     gene            12121..12942
                     /locus_tag="PC523_002057"
     CDS             12121..12942
                     /locus_tag="PC523_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475580.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNIAGTGKIEGSVKCSKFNSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGDINSVEIDTSGIINVEG
                     SIKSDIIRGEGYLKANENIDCENINLSGIMECNGFLNCEEVNISLNGISNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYIESKLFANIIEADEIS
                     LENSEVKVVRGKNVKIGKGCYVESLEYSEIIEIDEDSIVGEIKNINEKVKLKKDK"
     gene            12983..13750
                     /locus_tag="PC523_002058"
     CDS             12983..13750
                     /locus_tag="PC523_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HDF2475581.1"
                     /translation="MEKGSIKILGEGFISSGEYESIRIVGSAKSKGNIKSDEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLSIYGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVSENMYAQDIKIYGEMRNKSNIECEDIKVYGVIECDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDVFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSIFLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            13758..14378
                     /locus_tag="PC523_002059"
     CDS             13758..14378
                     /locus_tag="PC523_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HDF2475582.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSTITFEELKLIVNIIEENFTSIYNENGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEIDLTKEINDISIKLS"
     gene            complement(14680..15267)
                     /locus_tag="PC523_002060"
     CDS             complement(14680..15267)
                     /locus_tag="PC523_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2475583.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENINISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHVGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(15353..15790)
                     /locus_tag="PC523_002061"
     CDS             complement(15353..15790)
                     /locus_tag="PC523_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2475584.1"
                     /translation="MEDKYIVHFISKTKTNMIKFIENKLSKNDLDELIPTHGNILTAL
                     YENNGILTMKEIATKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            15894..16892
                     /locus_tag="PC523_002062"
     CDS             15894..16892
                     /locus_tag="PC523_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HDF2475585.1"
                     /translation="MNKKFKSLYITEEEGNFKRNIVERDVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVRLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKDMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKQALLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGTHVGRTVVDLEKI"
     gene            complement(17013..17939)
                     /locus_tag="PC523_002063"
     CDS             complement(17013..17939)
                     /locus_tag="PC523_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDF2475586.1"
                     /translation="MNKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMKGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSIGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            18325..19926
                     /locus_tag="PC523_002064"
     CDS             18325..19926
                     /locus_tag="PC523_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2475587.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKNVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETYDPEGGHIGRVEGSEEPNGYLEENAFFNVSSKIKPPSSNEIFNSIEKAQDIYLS
                     YGITTAQEGLMEENQFNLLKAMANQNKLKIDVVGYVNLKKSKPVADNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSKPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAAEQLIDVFEKVLTLKEQSSNNMRPVMIHAQTVREDQIDDMKRINMIPSYF
                     VAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAVN
                     RITKKGVQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPLT
                     IDPMKIKDIKVLQTIREGEVLYSLK"
     gene            20713..21984
                     /gene="serS"
                     /locus_tag="PC523_002065"
     CDS             20713..21984
                     /gene="serS"
                     /locus_tag="PC523_002065"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDF2475588.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKAFNLDEVVKLDDERRKILQE
                     VEVMKNKLNTASKNIPNLIKEGKDVENEKIKLKDLSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPGVPQGETDDDNVEVRSWGETTKFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYAHTLNGSGLAIGRS
                     VAAILENYQQEDGSVIVPEVLRPYMGVSVIK"
     gene            22257..23762
                     /locus_tag="PC523_002066"
     CDS             22257..23762
                     /locus_tag="PC523_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2475589.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELVVSNIV
                     NNFETSEILEFNKNNKIENTPVNYSENTLNLYAAVESIRGQFIDPFKIPLIIYDDYWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSANQFTCKYGITIYNISIIYKNNSIGV
                     IRGGYILMSDSSLGVGHRNLYDIPEGAARTMKRLLNQIAKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMVSIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIENYLNLQKLRYGDALKVKYLIPKKLYNLSVP
                     FNFIQPIVENAFTHGSRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDKNNIDKIKAG
                     IRINNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPSENYKEYV"
     gene            23876..24574
                     /locus_tag="PC523_002067"
     CDS             23876..24574
                     /locus_tag="PC523_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2475590.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     TGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFDYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIK
                     KGMTPKVYKLNTKN"
     gene            24858..25946
                     /locus_tag="PC523_002068"
     CDS             24858..25946
                     /locus_tag="PC523_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475591.1"
                     /translation="MSDKVNIKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     VGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIISGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNIAPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCREKSTQGQE"
BASE COUNT        10341 a         2665 c         4457 g         8611 t
ORIGIN      
        1 atttttaatc attaaattat gtaaaaaaaa tggatgcata gataactaaa tcatttaaga
       61 aaagattttt tagaaataaa aaatgataac taaagaaccc ctttaatatt tatcaaatag
      121 acaatcataa atattaaagg ggttctttag tttataaaaa ttatttgtaa taaagtagga
      181 tatattaata aatatgatag aatatatatt ataaattaaa tttataaata taagggggga
      241 actttgagtt cttatagatt tcttacacta taagaaccaa tactttatgc gggagttatt
      301 aaatgtaaca cttataataa tacttgtttt gataaatgct ttttttgtag cttgtgaatt
      361 tgctatggta aaacttcgta gttctaggat tgacaccatg ataactgaag gtaataataa
      421 tgcaaaaaga tgtaaaataa taaaagacaa cttaaattcc tatctttctg catgtcaact
      481 tggtataaca ctttgttcac ttgcattagg ttggatgggg gaatctacag ttaaagagtt
      541 gattttacca ataataagca tatttaatct tagtgaaagt attgtatata ctatatcaat
      601 ttccatttct tttttaatta taacaatgat ggaggttgta ataggagaat tggttcctaa
      661 agctttagca ctttataata cagaaaaaat aatgttgagt acatcttttt tagtagtagg
      721 attttataag ttaacatatc caataatata ttttttcaac ttaagtactg atttatttct
      781 taaaccattt ggatattcac aagctgatga agttgatgaa ccacatacag gtgatgaaat
      841 aagaatacta gtggaagaaa gttataaaag tgggcttata gatgaatcag agcaaagatt
      901 agttgataat atttttgaat ttgaggaaaa gaaaattaga gagataatgg ttcctagaac
      961 cgatatggta tgtatatatg aaagtgactc tgaagaaaag atattagcta ttttaaaaga
     1021 agaaggtgtt acaagatatc cagtatgtag aaaaaacaaa gatgatatat taggttttgt
     1081 acatataaga gatttataca atcaaaagat aaatgaaaat aaaattgaac tagaagagat
     1141 attgagagat attatatata tttctgaaaa tttgactata gataaagctc ttgagaggat
     1201 aagaaaggaa aaacttcaac ttgctatagt tgtggacgaa tatggtggta catctggtgt
     1261 ggtaacgata gaagatatac tagaagagat agttggagaa attcaagatg aatttgacaa
     1321 agaagagact catattaaga aaataggagc aggttcttac ttagttgatg gaactgaatc
     1381 cattaactat attaataagt attttggact tgacatagag catgatggat ttgacagtat
     1441 aggtgggtgg atatcatata tgcttggttc aaatattaaa gtaaatcaaa gtgtaacttt
     1501 tgaaaattac aatttcacaa tattagagtt ggataaattg agggttgtaa aattaatcat
     1561 aaaaagtgta atataattat atcaaaaaat attggaggaa ttgtatgcaa aaagtagaag
     1621 gaattatttt tgacatggat ggagttttat ttgactcaga gagaatttct cttgaatttt
     1681 ggatagagac gtttgaaaag tatggatata ctatgactaa ggaaatctat acatcagtta
     1741 tgggaagaaa tcgtaaaggt ataatagaag gtctcacaaa tatatacgat agttctgtac
     1801 caataataga tttgtatgat gaaaaaacta aaaatatgat tgaatttatg gatagggagg
     1861 gagctcctat caaattagga gtcaatgaac taatctcttt tttaaaggaa aatggataca
     1921 aaatggctgt tgcaacatca acaaagagag aaagagcagt aaagagatta gctaaggcaa
     1981 atttaaaaga ttactttgat gctatagttt gtggggatga tgttgtaaac tctaaaccaa
     2041 atcctgagat attcttaaaa gcagctaaaa agataaatgt taatccccaa aattgtattg
     2101 tgatagagga ttcgcctatg ggagttgaag ctgcatacaa tggtggtata agatgtataa
     2161 atgtaccaga tttaaaagag ccagatgagc aaattaaatc acaatcacat aagatacttg
     2221 aaaatttatt agaagttaga gaatacttaa aaagtttgag ctctaaagaa tgtcacaatc
     2281 tataatataa aatattaatt aaataaatta aatataattt ctttgattta gaataaatcc
     2341 atgaatatga gaaaaaataa gtataaacag ctaggaggaa tattcatgga taatgtaggt
     2401 tctctattat attttttatt taattttttc ttgtatggtt ttataggttg gataattgaa
     2461 aatttatata gttattataa agaaggacat tttcaagaag atggattttt aaatggtcca
     2521 tttaaaccaa tgtatgcaat tgcaatgtcg attataatag aggtatcaag atttattgac
     2581 ccaaataata tatttacatt gcttttatta tgtctagtag tgccaacagt agtagaatat
     2641 attacaggaa tacttatgaa agtatatttt aataaggtct attgggatta ttcagattat
     2701 aagtacaata gtagaggaat agtatgtcta aagttctcta tatattggac tatattgacc
     2761 tttattggag ttaggtattt tcaagtatat atagttgata gtttatatag tcttataata
     2821 ccttattggc caattcttgt aattatactt ttagcaactt tgtttgtgga tgaagttctt
     2881 actataaaat ttttgagggg aaataaagat atagtagaaa gataacttta aaaatataag
     2941 ttgaatttag attgtagtta aaagaaagct atgttaaaat ggtgaaatca tttaaaatta
     3001 gctttctttt ttatttgata aaattatttt ataatatagc tataagttgt aaagttttct
     3061 attaaaatta aaataaagtg tagaaaatgg ggagtgattt atgatgggta aagtaagatt
     3121 tggtattgtt gggacaagca atatagcaaa tatatttcta aaagctgcat ctaaggttaa
     3181 agactttgaa ttggttgcta tatattctag aaatttagaa aaagctagag aatttggaat
     3241 gtcacatgga gcaagtattt tttttgatga tttagaacaa atggctaaat cgaaggatat
     3301 agatgctgta tatatagctt ctccaaatgc attacattca aggcaagcca ttatttgttt
     3361 aaaacacaaa aaacatgtac tttgtgaaaa atcgttagca tctaatttaa aagaagtcaa
     3421 attaatgata aaaacagcca aagataataa tgtattgctt atggaagcta tgagaattac
     3481 atgtgttcca aactttagag cagttaaaga aaacttgtat aaaataggga aaataagaag
     3541 attttttgga agctattgtc agtattcttc cagatatgat aaatataaaa atggaattat
     3601 agagaatgca tttaaaaaag agttatcaaa tggagcttta atggatatag gagtgtattg
     3661 tatacatcct atggtaaatt tatttggggc tcccaaatct gtaaaggctg tttctcatgt
     3721 acttcaaact ggagtggacg gagaaggttc agctgtattt caatatgatg atatggatgc
     3781 tataattcag tattctaaag tagctgattc ttatatacca tctgaaatac aaggagaaga
     3841 gggaagtata ataattgaaa agcttaatct atttgaaaaa gctattatta aatacagaga
     3901 tggaagagaa gaagatatat ctattccaaa agaaaaagca gaggaaaaac ctagagaaat
     3961 agaaggaatg tattatgaac ttgtagagtt tataagtctt ataaataaca ataaaataga
     4021 atcaaatata aattcttatg aaaactcaat aatagtaatg gaaattatgg atgagataag
     4081 aagacaaagt agtattgttt atccagctga tagtatatag tttatattat aaagtatata
     4141 agaatttata atatatattc ttaaataaat ttaattttaa gttaaggtaa gatgtatact
     4201 tagttaagtg ttaaaatgtc aaattataag tatgaaaaag tgctaaaaag aaatttattt
     4261 ttggcgcttt ttttcttttt tttacaattt tgtaacagta tcaaaaaaag ttttgacgat
     4321 tttattacaa ttttgtagtt gaatatacat atcaaaacta atatgattaa taataacata
     4381 aatttgaata tttttagagg agagaaaata tgagaaaatt gatatacttt attacacctt
     4441 ttataatagg cgtagtattt ttatttggac tagacaaatt tcttgattct aaaactgatg
     4501 aacttttgag ggagaaaaat ctacttccaa ttatggatga tactttaagt gatataaaag
     4561 ataaaggagt tacagcaaat aatcattttt taagagaaaa agatattatg attttggggt
     4621 catcagaact tagtaattca acaaaacaac atccaaagta ctattttaat acgaatagga
     4681 gtaaaaataa ggtgtttgca attgggagag catatactca gacattacaa gatgctgcaa
     4741 tattaggaag tatgaatcct aatattgata ataagaaagt tgtcttgtta atttctatgc
     4801 aatggtttat ggaaaaagat ggagtcacaa gtcaccatta tcagtctaga ttttcaccaa
     4861 tacaatttta tcgattttta gataatccaa aaatatctaa acaaaataag attgagtatg
     4921 caaaaaaatc aagtaagtta ttatggggaa gtgacgagta taaagcagaa gcattatatg
     4981 ctaagctata tgagcctaag acgttgcttg aaaaagctga aaaggtgctt ttagagcctt
     5041 attttcaagg aagaaagtac tgtatagctc ttaaagaaaa gggtattctt tataaacgat
     5101 taattaagct agataaaaag agagcaacaa aaagaaaaag cccaataaat tggtcgcatg
     5161 agagaaagaa agccattgaa gatgctaaaa aaagagttgg taaaaatcca ctaaatattg
     5221 ataattatta ttataaacag cattttaaag atggtataga tcaatacaaa ggaagagaca
     5281 aagatgtaaa tttattaaca tctaaggagt ttgaaagtta taaacttatg ctaaatgttt
     5341 gtactgactt aggtattaag ccagttgttg tattaatccc aagtatggat aaattctata
     5401 atttgactgg tatttctgaa aaagaaagaa atcaatacta cgataaagca caaaacatag
     5461 cagaaagcaa aggctttgag gttctaaatc ttaaagataa agggtcagat aagtattatt
     5521 taagggatgt aatgcatctt ggcacgaaag gatgggtaga tgtctgtgag agattattta
     5581 aaatatttaa agaacaataa ggatttgtta actagtattt gtttaactat ggttataata
     5641 ttggcgatag gagttctaac atttactcca tttaaagaat tgccatttat ttataacgga
     5701 ttttaaagga gcattcttat atgaaaatta ttgaaggaat aaagaaatat tcaaatacag
     5761 atagaatagc actaatatgt aatggagata aactttctta taaagattta aatgagtatt
     5821 cagatgctat atcaatattt ttaaaagatg tatacaaaga agaagatact cctatagtaa
     5881 tttatggaaa taaagaaaat atgataatgg catgtatgat aggagcttta aaatcaggga
     5941 gagcgtatgt gcctcttgat attagttttc caattgatag agtttttgaa gtcactaaag
     6001 agataaaacc aaaagtatta tttaatttta gtgatgaaaa agattttgga gatattaatg
     6061 taattgatat ggataaacta aaggatataa taaatgaata tcagggaaaa tcactagata
     6121 aagaaaactg ggtaaaagat gatgaaaatg catatatatt atttacctca ggaagtacag
     6181 gaaaaccaaa aggggttcaa ataagttcaa acaatttgga tagcttttcg gattggataa
     6241 gcccatatct aaacatagat ggaagtgaaa aagtaataat gaatcaacca gcatattctt
     6301 ttgacctttc agttacaaca atatatcctg gtcttatcca tggagctaca ttattttcaa
     6361 tatcaaaaga tgtattggca gactataaag aactatttag acaatttagt atatcagata
     6421 tagctgtatg ggtgtcaact ccttcttttg caggggtatg tataactgaa aaagagttca
     6481 atagtaaaat gcttcctaag cttgaatcaa tgatattcat aggagaagca ctttcaaaaa
     6541 atcttactaa agaacttatg agcagatttc caaacacaag agttatcaat ggatatggac
     6601 caacagaggc tactgttggt gtgagcgtaa atgatatgac tcaaaaagct atagaggatg
     6661 aaaaaagtct tccagttgga tatcctatgt caaattgtaa aataaaaata cttgatgaag
     6721 ctggaaatga acttaaagaa aatgaaaagg gtgagattat aataattgga ccatcagttt
     6781 ctaaaggtta cttcaataat aaagaaaaga ctgatgaagt atttttctat gatgaaatag
     6841 atggtataaa gtggagagct tataaaactg gagatatggg atatctttta gatggaaata
     6901 tatattattg tggaagaaaa gattttcaga ttaaattaaa tggatttaga attgaaatag
     6961 aagatataga aaataatttg agaaaagtac ataatgtaaa aaatgtagtg gttttaccag
     7021 tatataagga cgaaaaaatt gcttacttaa aaggtattgt tgagttaaat gaaaagaatg
     7081 atttaagtaa tattaaaaat gggatgataa taaaaaagga acttggaaag tatatacctt
     7141 catatatgat tccaagaaat atttcaataa tctctgaatt tccaacaaat atcaacggta
     7201 aaatagacag aaagaaactt atggaggaaa tttaatgatt ttttcacagt atggagatta
     7261 tttttatctt tatatattat tattaacatc aataccagca gtaattttag gtctaatggg
     7321 taagaatata aagtattatg gtatgttggc aagtttgttt atgatattcc taatagtagg
     7381 aattgatgtc caactaaagt acttagtaat atttattatt cttgaagtaa taatagttaa
     7441 aggatatgaa tatgtaagaa gaaaaactaa aaataaatac gtatattgga gctttttgtt
     7501 tatatcaatg cttccaatca taatcaataa aatatctcca gttacttctt ttggaatgat
     7561 tggatttatt ggaatatcat acttaaattt cagagctata cagatggtta ttgaaattta
     7621 tgatggtgct ataaaagaag ttaaaatttc aaagatgctt tattttatgt tattttttcc
     7681 aactttaagt tctggaccta ttgataggtc aagaagattt gaacaagact tggaaaaaca
     7741 aatttctaga aaagagtata tagaagaata tttactgcca ggaataaaaa atattgtcat
     7801 gggagtaggt tataagtttg ttatagcatt tttgataaat acatattggg tatcaataat
     7861 acctaaagat atgagtttta taaatatttt aagttatatg tacgcatata gcttgtatct
     7921 attctttgat tttgcaggat atagtttatt tgctataggt acaggttaca tatttggaat
     7981 acaagtacct ataaactttg acaagccatt tatcagtaaa gatatgaaag agttctggac
     8041 aagatggcat ataagccttt caagatggtt tggagattat attttttcta gatttgttat
     8101 gtcatcaatg agaaagaaga gatttaagaa aagaactact gctgctcatg tggctcagat
     8161 gataactatg ataacaatgg gattttggca tgggctaact tggtactacg ttgcttatgg
     8221 tgtatatcaa ggtttggcac ttgttctaac tgatatatat cagagaaaat ccaaatttta
     8281 taaaaaacat aaaaaagaaa aatggtttga gagagtacag attttcatta cattccacat
     8341 tgtatgtttt ggattactga tattctcagg ttatttagct taactttaag gaggaaaaat
     8401 tatgcaagag acagttatag aaatatttga agatgtatta gggactgatg agataagaga
     8461 agatttagac cttaacttat ttgagacaga gcttttagac tcattagcta taattgaagt
     8521 tttacttgaa attgaaaata gattaggaat agagcttcaa ccaactgatt tagagagaaa
     8581 agatatgtct actgtaaata atttagtaaa attcttggaa acaagaaaat aaattagtag
     8641 ttagattaaa tattaattta taaaataaaa ttttacaaag tccatctgat actaatattt
     8701 gatggacttt tttaatgata atacatagtc ttttgaagac aactttaatt aagtaaaaaa
     8761 tttaatatat aaatatgatt ttgtaaatct taggtatcat tgtaatatta taggagaaaa
     8821 atatattata tttaggagga aaatgaatgt ctttaaattc aatagataga aaaaaagaaa
     8881 tactgaaatt attagattca aaaggtaagg taaatactaa agagttagta aaaagactag
     8941 aagtatcatc agaaacaata agaagatatt tggaagaatt agaaaatgaa aataaattaa
     9001 aaaaggtata tggtggtgct ataaaaataa attatggggt gattgaacca gaacatataa
     9061 agagaaatag tactaattta gataaaaaac aaagtattgc taaatatgca tctaagtttg
     9121 tgaatgacaa tgagtcaatc atcatagatg aaggtactac caatttgcaa attgtagatt
     9181 atataatatc tgtaaacaat ttaaaaatta ttacaaattc ttatccagtt ctatcaaaat
     9241 tgatttctta tgaaaatcaa ggcttatttg atggggaagt tatatttatt ggtggaaagg
     9301 taaatttaaa acatcaaaga acagcgtgtc caatgtcaat agatactatg aagaacttat
     9361 acgtagataa agcttttata tctagcgaag gtatacttga tagttttgga attagtagta
     9421 tagaccaaaa taaggcattg ttgtcaaaag aatatataaa aaattcaaag gaaagttttg
     9481 tattgtgtga tagttctaaa atcggaattg ctagtctgta taagatagca gatttagagg
     9541 aattagattt tgttatttgt gatgtagagc ctccgaatga atggaaggat aagttaaatg
     9601 agataaatat aacatggata gaagcaaaat aaacccttag ttttatagaa tacaactatg
     9661 cttaatacaa ccaaattcat aaaatatagt cagtttatag attaatatta gaaagtaaat
     9721 tttagattgt aaatttagaa ttttatagaa atatgacata gaataaaaac aacttaaaac
     9781 aaataaaaac aactaaaaac agaaatttaa tatatattta acttaaatat gatgtataat
     9841 taacatatga aagttatact ttaaaagtaa aaacaattaa aaacacaaaa attctaaaaa
     9901 gaatctatgg aggctataaa tatgaaaaag atatatggag aaaaaataaa agcagtagta
     9961 ttcgattggg ctggaacaac agtagattat ggatgttttg cacctcttaa tgtatttata
    10021 gagatattta aaagaagagg catagatgta acaatggaag aagcaagaaa acctatggga
    10081 aaattaaaaa tagaccatat aagagaaatg tgtgaaatgg atagaataaa aaatctttgg
    10141 tcagataagt ttggtaaagt gccaattgaa gacgacgtaa atgaacttta tgctgaattt
    10201 gagccaatgt tatttgagac attagaagag tatactactc caatccccca tgttattgaa
    10261 actattgaaa aattaagaga aaatggatta aaaataggct ctacaacagg ttatacaaga
    10321 gagatgatgg atatagttca accaaacgct gcaaaaaaag gatactcacc agattttctt
    10381 gtaacaccat cagaagtatc acaaggaaga ccatatcctt ggatgtgcta taaaaatgct
    10441 gaagctttag gagtatctcc tatgagttct atggtaaaag taggagatac aataagtgac
    10501 gtaaaagaag gcgtaaatgc aggaatgtgg agtgttgctg taataaaggg ttcaagtgag
    10561 ttagggctta cagaagaaga agtagagaac atggataagg aagaattaaa aattaaaatg
    10621 aatacagttt caaagaaatt taaagaagca ggtgcacatt ttgtaataga aactatggct
    10681 gaattagaag atgtattggt aaagatagaa aataaaacta ttaaatcaga ctttgtacca
    10741 gaaaatgatt acatattact tacaccaggg ccactatcta ctactaaatc ggtaagagca
    10801 tctatgttaa aagattggtg tacttgggat gttgaatata atgacttagt acaagatgtt
    10861 agaagaagat taatttcttt aggaacaaag aatacagaca aatacacaag tgtattaatg
    10921 caaggaagtg gtactttttc agttgaagct acaataggta gtactattcc agagaatggt
    10981 aaacttcttg taattgcaaa tggagcatat ggaaagagaa tgaaagatat atgtaattat
    11041 cttaacatag aatttgttga ttgcacattt aaagatgtag aagcggtgga tttaaatgta
    11101 gtagaaaatt tattaaaaga aaataaagat ataacacata tatcaatggt tcactgtgaa
    11161 actactacag ggagattaaa ccccatacaa gaggtaggga aacttgcaaa agaatataac
    11221 aaagtttata tagttgatgc tatgagtagt tttgggggaa ttgagattga tgttgaagat
    11281 tttaatatag attttttagt tagtagttct aacaaatgta tacaaggagt acctggattt
    11341 ggttttataa tagctaataa agaagaatta tctaaatgta aaggtatagc taaatcatta
    11401 tctcttgatg tatatgcgca atgggagact atggagaaaa ataatggtaa atggagattt
    11461 acttcaccta cacatgttgt aagagctttt tatcaagctt tattagaatt agaagaagaa
    11521 ggttctgttg aaaaaagata tgctagatat aaagaaaatc aatttacaat agcttcaaga
    11581 ttaaaatctt taggttttga tactttagtg aatgataatg ctcaatcacc tgtaataaca
    11641 actttcttat atcctaaaaa tgcaaagttt gaatttatgg aattttatac ttatttaaaa
    11701 gctaatggat ttgttatcta tccaggtaaa ttgactgata tagatacatt tagaataggt
    11761 agcataggtg aagtttatcc aactgatatg gagagactag cagatgtaat agaaagattt
    11821 atcaatagat agaagaagat agtggcttgt aggttactat agaatttaaa gacttatatt
    11881 ttagatgttt ataaaatgaa tatataaaga ttgagtaata gggatatatt ttacttaaac
    11941 cacaatctaa gaaattagat ttatagttta gataaagtat ttttcctatt attttttgtg
    12001 tttatttata taaaataaat tatggttatt ttattaagta ttattatcat acttgacaac
    12061 gtaacaatgt tttgttatat taagcttaga gataaaatac ggtaaagaaa ggatatcatt
    12121 atgaatgaag aattaagttc actaagattt gatggcgttg gaaaatgtaa aggtggaatt
    12181 tttgataatg ttaatatagc aggaacagga aaaatagaag gaagtgtaaa atgtagtaaa
    12241 tttaattctt ctggttcagc aaaagtgttt ggtacaacag aatgtaatga attttttaca
    12301 tctggtttaa gtaaagtaga aggaaatata ctggcatcaa aagttgagat atcggggctt
    12361 cttagatgta caggagatat taattctgtt gaaattgaca ctagtggaat tattaatgta
    12421 gaaggaagta taaagtcgga tattattcgt ggagaaggat atttaaaagc aaatgagaat
    12481 atagattgtg aaaatatcaa tttaagtgga atcatggaat gtaatggatt tttgaattgt
    12541 gaagaggtta atattagttt aaatggaatt tctaatttta atgaagttgg agcaagtagt
    12601 ataaatataa aaaaaggtca tggagattat tgcaaaaaat ttcgtatgct agaaaaaaag
    12661 catatcaact ttgaaaaggt atcttcaaat ttaataagtt tgggtttatt atcaattaga
    12721 aagtatatag aaagcaaatt atttgcaaat attatagaag cagatgaaat aagcctagaa
    12781 aatagtgaag tcaaagttgt aagaggaaaa aatgttaaaa taggtaaggg atgttatgta
    12841 gaaagcttgg agtatagtga aattatagag attgatgaag attcaatagt tggtgaaata
    12901 aagaatataa atgaaaaagt aaaattaaag aaggataagt aataagtagg tttaaaaata
    12961 aaatttaaaa tgaggtaata atatggaaaa agggtctata aaaatacttg gagaaggctt
    13021 tataagtagt ggtgagtatg aaagtataag aatcgtggga agtgctaaat caaaaggaaa
    13081 tatcaaaagt gatgaagtaa aagtccttgg agatgcaaaa tttgaaggtg acatcaatat
    13141 aaaaaatttt gttgttaatg gaaaaactat tataagaggc aatcttaaag cagagcgtgt
    13201 aagagtgaat gggcaactta gcatatatgg agatgctgaa atagatgact tagttataac
    13261 tgctcaacag aaagtggatg gcaaacttaa atgtaataat gttacaataa gaggacaatt
    13321 aagtgtaagt gaaaatatgt atgcacaaga tataaaaatc tatggcgaaa tgagaaataa
    13381 atcaaatata gaatgtgaag atataaaggt atatggagta atagaatgtg atggcctttt
    13441 aaatggagaa aatatatatg tttattcagg aggaggttct tattgtaaag agataggtgc
    13501 gactaatata aaaataggaa gaagcaaaga tgttttttca ttaattaaaa ttattaatat
    13561 tttttcagga aagtttaaat gcaacttgat agagggagat actattgaat tagaagatgc
    13621 atcaataaaa gatatacgag gtaaaagcat atttttagta gataattgtg atgttggaaa
    13681 tattgaatac agtgataatt tgtcagtttc agataattct actgtaaaaa acagtataaa
    13741 ggtgggctag aagagttatg gaagaattaa tatctaagaa agatttattg agcaaaacta
    13801 atatatctta tgggcaatta tatagatgga aaagaaaaaa cataatacca gaagaatggt
    13861 ttataaagaa atctgctttt acaggtcaag agactttctt tccaagagat aaaatactag
    13921 aaagaataga gttaatattg tctatgaaag aagagacttc tcttgatgac attgcaaata
    13981 tgtttatgaa aaaagatatt aataaagaat ttgatattga ttttattctt agtaaagaag
    14041 ttatatgtaa ttacaccaaa gaaatatttc aaaatttata taagaaagag caaagtatag
    14101 gtaaaaaaga acttcttata ttgagtataa tagagaagtt tttagttaaa tctacaataa
    14161 cttttgaaga gttaaaattg atagtgaata taatagaaga aaattttaca tcaatatata
    14221 atgaaaatgg aaagatttat ttgtttagaa agtttggagt tccatttgta gttggatgtc
    14281 tggattataa aaaagtaaaa tttgaaaaag atgtagttaa gattattgag attgacttaa
    14341 caaaagaaat aaatgatata agtataaagt tatcgtagtg tgtattatct tatataatgt
    14401 attacaataa tctacctaaa atacacttta tttaaatatt gggtttaaag atgagttttt
    14461 atgtgaatat gtaaaactta aagatagatt taagcaatct atagggatta gtaaacaata
    14521 aaatgaacca tgatgaagta ctataaattg tatttaaact acattttcta cttatagtaa
    14581 tatcaatggt tcattttatt tttattaaga taatacagta tttataaaaa tacttaatat
    14641 aaatatagca aattgtttaa ataaaatgat tatcttagtt tattcaaata atgagttttt
    14701 aaaatctaag aaatatcctt gtggatgaag acatacagga caagcttttg gagcttcttt
    14761 acctacatgt atatgcccac aatgtgtaca tatccattgt atatcttcat ttcttctgaa
    14821 taaagtacct tcttctaaat cagtagcata tctaccaaat ctatcaccat gaactttttc
    14881 tatttcagct atattttcaa ataacttagc tatagattca aatccttctt cacgtgctgt
    14941 ttttccaaag tttttatata catcatccca ttcttgaagc tcattatgtt gtgcagcttt
    15001 aagatgttgt gctgttttat catataaatc tacagggtat gcccctgata tatttatgtt
    15061 ttcaccagca aattctttta gtttagaaaa gaattgtttt gcatgtgatt tttcttggtc
    15121 agcagtgtaa ttaaataaag attctattat atataatcct tctttttttg ctacagatgc
    15181 agaaatattg tatctatttc tagcttgact ctcacctgca aaagctctca ataagttttc
    15241 tttagtttta ctatctttta aattcatatt ctacctctct atcacaataa atttttacaa
    15301 tatttgattc tttttattta ttattccaat agaagatata attatgctta atttagtcag
    15361 catttttaaa attatcatta atttttttta aaattctaag aaattcttgt ttttcatttt
    15421 ctgtaatatt atggtatgct gtttctttaa cttgtgttga gatactatta aatgtatctt
    15481 ctatgagtag tgcttttttt gtaagcttta tatatgtgat acgtttgtca ttggtacatt
    15541 tttgtctttc tagatatcct agatttatga gtttattaac caacactgta acagtggatt
    15601 tgtcctttcc tattttagtt gcaatttctt tcattgtaag tattccatta ttttcataaa
    15661 gtgcagttaa gatatttcca tgagtaggta tcaactcatc taaatcattt ttactgagtt
    15721 tattttcaat aaatttaatc atattagtct ttgttttact tatgaaatgt actatatatt
    15781 tatcttccat aaattattct ccaatcttta atctatttaa attagtttga cataaaacta
    15841 tttataaact ataattagtt taacataaaa ctaaatgtat tgggaggaaa tttatgaata
    15901 aaaaatttaa atcactatat attacagaag aggaaggaaa ttttaaaaga aatatagttg
    15961 aaagagatgt agatacattg ccagagggag aagttttaat aaaagtgaaa tattcttctc
    16021 taaattacaa ggatgcactt tctgccaatg gtaataaggg tgttactaga aaataccctc
    16081 atacaccagg aattgatgca tctggtatag tagaatctag tagttgtgat gattttaaaa
    16141 ttggagaaga agttttagta acaggttatg acttgggaat gaatacttct ggtggatttt
    16201 cagaatatat aagagttcct tccagttggg tagttagact tcctaaaaat ctttcactaa
    16261 aagaagcaat ggattatggc acagcagggt ttacagcagc tttatctgtg tacaaattta
    16321 tagattctgt agacaaggac atgggagatg tattagtaac tggtggtact ggaggagttg
    16381 ctgtaattgc aaccaaaata ttgattaaat taggttattc agttgtagtt tcaacaggaa
    16441 aactagaaga acaaaaacaa gcattactaa acttaggtgt taaagatgtt atacataggt
    16501 cagaagtgga tgataactca ggaagacctc tacttagacc aagatgggct ggagtaatag
    16561 atacagtagg aggaaatacc ttagcaacag caataaagac aactaattat tgtggagcag
    16621 taacaacttg tggtaatgca ggaggagttg attttacaag ttcagtatat ccatttatct
    16681 taaagggaat taccttgtat ggtatagatt ctgtggactg cccaatgaat ttaagattaa
    16741 aaatatggga tttattatca aatgaatgga aattagatga tttagacgat atggtaaatg
    16801 tggtatcatt agaagaatta aatgacagta tagattcaat aatggcagga acacatgtgg
    16861 gaagaacagt agttgattta gaaaaaatat aggtgactaa gtttgcaatt aggattttgg
    16921 gttttaagca aaaatgagat acttgtaatt tacttatgag tatctcattt ttttatgtaa
    16981 aaaaatttta tgtagaagtt tgtgtaaaca ggttattcat gaacgattaa ttcaaataca
    17041 tctggtcttg aatagtgccc ttctgaatca aaatctagtc tgctaagaac tattttttct
    17101 aggtctaaat cagctattag catttcttct ttattgaaaa tagggccagc tacatatttt
    17161 ccaaaagggt caactataca actccctcct ggacacataa tttctggttc tgcatctagt
    17221 tctttatagt aattaaggtc agtaggatac atgttttttt ctacatattg attacatcca
    17281 attacaaaac atcttccctc taaagcaata tgtttcatag ttgcttgcca ctcttctctt
    17341 gaatctgcag taggggctat ataaagtttt acacccttag cataaagagc tgttctagct
    17401 aatggcatat aattttccca acaaatcaat gaacccattt ttccatatgg agtatcaact
    17461 actgtaagag tacttccatc tccttctccc catatacatc tttctgttcc tgtaggttta
    17521 agttttctgt gtttaccaat tagcttacct tctggagaga aaaataagtt tgtgcaataa
    17581 agggtacaat ttatatcatt accatccctc tctgttatac ctattgatag atatactcct
    17641 gcttcttgtg ctgcttttcc taataaatct gttgtagcac taggaactgg tacagaatta
    17701 tcatagtatc ttttccaatc ctctctacct ttcattgttc tacttccaac aacaaatcca
    17761 aatgaaagac ctcttggata tgcaggtatg aatgcctcag ggaaaactac gatatttgcc
    17821 ccttttctac cagcttcttt aattaatccc aatgcttttt caacagtttt ttctaaatcc
    17881 attatgactg gtgatgcctg gacaacagca attcttacat ttttattttc cttgttcata
    17941 aaaaacctcc taaaaaatat ttaaatttgt tgcattgaat agactacatg agatgttatt
    18001 tgagaacgaa gatatttata ttgtgaataa taatataact gtatatttaa aataatacat
    18061 catgatttaa aaaattacaa ttgtacatat ataattgagt ttagatattt aaatttaata
    18121 acttataata ttttttcaat tagtataaag cctattaatt tgtcttattt acaatgaata
    18181 gtatttactt cttggcaaac aataattaaa aataaaactt ttatataatt aaaatctaaa
    18241 gaaaaagttt ataaagaaga taatataatt aagtagaaaa taaagaaaaa aatttattaa
    18301 agtagacaac ataaggagaa ttttatgaaa gaaaaaattt attacaatgg aaatatcatc
    18361 acaatggaag attcaatatg tggagatgcg attcttataa aagataagat aataaaaaag
    18421 ataggaacaa aagaagaagt atttgctttg aaaaataaag atacagaaat tatagatttg
    18481 caaggtaaga cattgatgcc atcttttata gactcacata gccatttgat agcttttgct
    18541 actactctaa agttagttcc tttagaagat gcaactagtt ttaaagacat agtaaaaaaa
    18601 atacaggatt tcaaagagtc aaataatata aaaaaaggag actggataat tggattttca
    18661 tatgataata attttttaga agaaaataaa catccagata agaatgtact cgacagtgcg
    18721 tcttcagaaa atccaatact aatttcgcat gcttctggtc atatgggagt tgcaaataca
    18781 ttggggttaa aacagcttgg tgttactaat gaaacatatg acccagaagg tggacatata
    18841 ggtagagtag aaggcagtga agaaccaaat ggatacttag aagaaaatgc attttttaat
    18901 gtatcatcta aaataaaacc accatcatca aatgagattt ttaattcaat tgaaaaagca
    18961 caggacatat atctaagcta tggtattaca acagctcaag aaggtctaat ggaagaaaat
    19021 caatttaatc ttttaaaagc aatggcaaat caaaataaat taaaaattga tgttgtagga
    19081 tatgtaaatt tgaaaaaaag taagcctgta gcagataaca atagagagtt tataaaaaaa
    19141 tatataaaca gatttaaaat aggaggctat aagatattct tagatggttc tccacaaggt
    19201 aagacagcat ggttgagtaa accgtatgaa aattcagatg atggttattg tgggtaccct
    19261 atatacaaag atgaagaagt agaaaagttt atagatattt cactaaaaga aaagatgcaa
    19321 ctcttaactc attgtaatgg agatgcagca gcagagcagt tgatagatgt atttgagaaa
    19381 gttttaacct taaaagagca aagtagtaat aatatgagac cagttatgat acatgctcaa
    19441 acagtaagag aagaccaaat tgatgatatg aagagaataa atatgatacc ttcatacttt
    19501 gtagctcaca catattattg gggtgacatt catatcaaaa accttggaga agatagagca
    19561 tttaagatta gcccacttaa aacaactata gaaaaggggt taatctacac tcttcatcag
    19621 gatacaccag taatagcacc taatatgctg gaaacagtat ggtgtgctgt caatagaatt
    19681 acaaaaaaag gtgtacaaat aggagaaaat gaaaagataa gcccattaga tgccttaaag
    19741 ggtgttacta taaatgcagc atatcaatat tttgaagaag ataaaaaagg ctcaattaaa
    19801 gaaggcaaat tggctaattt gataatatta gatgaaaacc ctcttacaat cgaccctatg
    19861 aaaataaaag atattaaagt attgcagact ataagagaag gagaagtatt gtattctttg
    19921 aagtagatgc aagttagata ttttataatc tataaaaagt agttttatat aataagttag
    19981 gtagaattaa ataagattgc agttaaatca acaattatat ttattattaa attttaatta
    20041 ttgtattgaa gatttaccag tataatttta ttgattctac ctttttaaag aactatcaat
    20101 ttattaaagc tatttaaaat acattaaaca attaagtaat tattagttgc agttattagt
    20161 ataaaacgaa tatgtatatt actttgcagt agattttgga gctaagttta taatagcaaa
    20221 tttaaattca ttaaatagat taccttacaa catttcatat aaaacatatt gtgaatctgg
    20281 ggaaattact acttttcttt gagtatttct tataatcagt tctaaaaaat ttaatttcat
    20341 acctatactt aaatcattta ttaaaataat actatccaaa taagagatat ataggtctat
    20401 attataatat aaaaaaactt atatgaaagt taataactat acaagattga taaagaaaaa
    20461 gtcaaaatag ataattgatt agaaaaatcg atataattat aatgaagtta aatctagaaa
    20521 tattctcttg aaattaaatt gtatttattt aattaaatat aatttgacaa tagataaaaa
    20581 agtgagtaaa atataaataa aaataatagg ttcggataaa aaaaggtttc ttaacctcaa
    20641 gcataaaaca tattatctaa gttatttgag attaagagaa aaaatccaat cttaggtact
    20701 gaggaggaaa aaatgcttga tataaaaaga ataagagaaa atttagattg tataaaaaaa
    20761 gcaatggaaa gaaggggaga aaaagctttt aaccttgatg aagtagttaa gttagatgat
    20821 gaaagaagaa agattcttca agaagttgaa gttatgaaaa ataaattaaa tacagcatct
    20881 aaaaatatac caaatcttat aaaagaaggt aaagatgtag aaaatgaaaa gattaaacta
    20941 aaagatttat ctgataaaat taaggtaata gaccaaaatc ttaaagaagt agaagacaag
    21001 atggaatatc tattaatgag aataccaaac gtaccacacc caggagtacc acaaggtgaa
    21061 actgatgatg ataatgttga ggtaagaagt tggggagaga ctaccaaatt tgattttgaa
    21121 tctaaggctc attgggaaat tggaacagaa ttaggtatac ttgattttga aacagctagt
    21181 aaaataaccg gttcaagatt cactctttac aaaggcttgg gtgctagatt agagagagca
    21241 ttattaaatt tttacttgga cacgaataca aaggtaaatg gatacacaga agttattcca
    21301 ccatttatgg caaatagaaa tagtttttta ggtacaggac agttacctaa gtttgaagaa
    21361 gatatgttta aaatagaagg actggactat tttatgattc caacatcaga agttcctttg
    21421 actaatatac atgctaatga aatacttaaa tttgaacaat taccaataaa ttatacagct
    21481 tatactcctt gttttaggtc agaagctggt tctgctggaa gagatacaag aggtttggta
    21541 agacaacatc agtttaataa agtagaaatg gttaaaatag ttgcaccaga agaatcttac
    21601 aatgagttgg aaaagcttac taataatgcg gagactatgc tacaattatt gaatctgcct
    21661 tatagagtag taaagatatg tacaggtgat ttaggattta cggcttcttt taaatatgat
    21721 gtagaagttt ggatgccaag ttacaatagg tatgttgaga tttcatcttg ttcaaactgc
    21781 gaagatttcc aagctagaag ggcaggaata agatttaaaa gagataaaga ttctaaagct
    21841 gaatatgctc acacattaaa tggttctggg ctagctattg gaagaagtgt tgcagctata
    21901 ttagaaaatt atcaacaaga agatggctct gtgattgttc ctgaagtttt aagaccatat
    21961 atgggagttt ctgttattaa ataagattca aattatgtat ttttaagcaa cacttctaat
    22021 atagaagtaa taaaaaataa ttaacataga agtgagacta taatctataa aaaataatta
    22081 atataaaagt aatacaataa tatagaagta attaaaaaat aataattaat ataaaaatgt
    22141 gtacaaacta atgaatattg atatagtaag ctatcatatg aaagagtatg tacacatttt
    22201 tgtttactaa ttttttataa tatgaacata aataatttac aggaaaggaa tattttatga
    22261 aaattcaaaa tactgagtgg ggatatattg aatggaaaca tacttatgat gaaaataatc
    22321 caaaacaagc tatgaatata tacattgcag taactatgcc tggtaagaaa cattttaatc
    22381 atgtacatta tggtcaagaa cagatgatat atattttaga gggagagggt ttgtatatta
    22441 taaatggggt ctggaagcca ttttatcaag ggatgatttt ttatatagaa tctggtagca
    22501 cacatgaaac tataaataca ggagatagag aaataaaaga gttagtagta tcaaatattg
    22561 taaacaattt tgaaacaagt gagattcttg aatttaataa aaataataaa atagaaaata
    22621 ctccagtaaa ttatagtgaa aataccttaa atctatacgc agcagttgaa tcaatacgag
    22681 gtcagtttat agacccattt aaaatacctt tgattattta tgatgattac tggaatatag
    22741 ttttaaaaaa tccatatttc cctttatttt gttttgaaaa atgtaatcca atgaaattcc
    22801 cacaaaattg tgattgcatg aatcaaaaaa gtgctaatca atttacatgt aagtatggta
    22861 taaccatata taatatttct ataatatata aaaataatag cataggggtt ataagaggtg
    22921 gatatattct aatgtctgat tcaagtttag gggttggaca taggaattta tatgatatac
    22981 cagaaggtgc agctagaact atgaaaaggt tactcaatca aattgctaaa aatataatta
    23041 atttttgttc atttaatgat ataagaaagg atttacaaga aaaagagaag actatagcaa
    23101 gaacatatca ttatggagag caacttgaaa tgaatttaaa ggtagctcaa gatatggtta
    23161 ctaatttaag aataaatcat cactttttat ttaatacctt aaattcaatg gtaagcatag
    23221 cactagatga tggtagttat gatttgtata gtgcaataat agatttatca agaatgttta
    23281 gatatacaat gaggtctgat ttaagatttg tagaattaga atctgaaata ttatacattg
    23341 aaaattattt gaatttacag aaattacgtt atggagatgc acttaaagta aaatatctta
    23401 ttccaaagaa gttatataac ctttctgtgc cttttaactt tattcaacct attgtagaga
    23461 atgcatttac acatggatct agagatattg acacagaaaa gagaattgaa ataattgcaa
    23521 gattagattc tcagtatgct attatagaga ttcataataa tggaactatt ttagataaaa
    23581 ataatataga caaaataaag gcaggtattc gtattaataa tggtcatggt ttgtctttaa
    23641 tatatacaaa attcacatct gcttatggaa ataattttga tatggatata aagtcgagtg
    23701 ataatgaggg aacttacata atgattaaaa ttcctagtga aaattataag gagtatgtat
    23761 agaacttgtg aaaaattaat aagttttata aatgttaaga aatatattag atgtttttat
    23821 gttgttttat atatcttact tttttatatt cttaagctaa aagggaggta ttctcatgag
    23881 aagagcacta ataatagatg atgaagaagc tgttctaaaa ataataacac attttataga
    23941 aatgaaagat atgccaataa aaattgttgg taaagcaacc tctggtgatg aagcagtaga
    24001 taaaatcact ggtttagagc cagacatagt ttttatagat attcaaatgc caatatataa
    24061 tgggttagaa gtaatagaaa aaacatctca cttatctaaa aaaataaact ttgtagtaat
    24121 aactgctttt gactattttg aatatgctca aaaagctttg agattaaatg taaaagatat
    24181 actccttaaa ccattggaca tgaaagaatt tactaaatct gtagaaaaaa ttattggata
    24241 tcaatatacc aataatgatt tattaaatga gatactggaa tatataaatc taaattatag
    24301 taatgactta aagttaaatg attgtgctag attattttta accaatccat ctaatatatc
    24361 taggattttt aaaagtaatt tgaatacaac ttttatatca tatctaaatc atataagaat
    24421 agaaaaatct attgagttac ttgaaaatac tacaaaaagt ataaatgaga ttgcagaaat
    24481 agttggatat aatagcctaa ataactttta taaaaacttt aagataaaaa aaggaatgac
    24541 tccaaaagtt tataaactga atactaaaaa ttgaaattga taaataaaaa atcaatatag
    24601 ataattgttt gagtatgtct gaataagtat actttaatta taataaaaat taaagcgctt
    24661 taaacatatt taataatatt aaaccttata aaagctgtac ctatttatat aaaaaaatat
    24721 atataaataa aaagaaaaag atatacaaaa taaaaaagta aatcaaatac tattctaaaa
    24781 agacagaatt ttcatttaat ttactgtgag tttatatttc ttagaggaag gtaactttat
    24841 aatggaggta atttaatatg tcagacaaag tgaatataaa gaatattatc tgtattgcag
    24901 gtgcttattt agcgacagca attggttctg ggtttgctac tggtcaagaa attctacaat
    24961 tttttgcctc acaagggtat atgggtctag ttggagcatt aatatcaatg gtgttgtttt
    25021 catttcttgg tgctgaagtt ataactagag gtagagaatt aaaacttaaa gaaccaacaa
    25081 gaatatacac tttttattgt ggtaagattt taggaaaatt ttacgaatgg tttagtccaa
    25141 tatttttatt tgggattctt atagtaatga tttcaggtgc aggttcaact ttaactgaat
    25201 attatggtat aaatccttac ttaggaagag tgttgatggc tgttgctact ttaattacag
    25261 tgtctatggg tttagaaaaa ttagtaagta tacttggtcg tattggacca gtgatagccg
    25321 tatttacaat tgctgttgga ttaataagct tgtttaaaca ttttgatggt ttatctatgg
    25381 ttggagaagc tatgaaaaac attgaagtta caaaagcatc agaaagtcca atcatttccg
    25441 gtgtaatata taatacatta aatattattg taatgattgt ttttttaaca ggaatgggag
    25501 cttccattga aagtaaaaag gatgcttttt ggggaggttt attgggtgga atcgtgttta
    25561 tgactgctgg tatagttatg tatttggcta tgatttcaga tataggaaat ttgtacatga
    25621 aagaaatacc atcactttat ttagcagata atatagctcc tattgtaggt gtatgttttt
    25681 cagttgcact aatattaggc atctatacaa cagctgttcc acttttatgg tctgtaacca
    25741 atagaattgt tgaagatgaa caccctaaat ttaaattagt aacaacagta atagctattt
    25801 tggcttgtat aggtggtttt ttaccgtttg gtaaactagt aaacatttta tatccatata
    25861 caggttatat gggactttta attttagtct gtatggtgta taaagcaatt acctgtagag
    25921 aaaaatcaac acaaggtcag gagtagagcc acttattagt aatttttagt taaagaaact
    25981 ttttatataa tcgaatttaa aaagtcaaaa tagttaaaaa aattgtaaaa tatgataata
    26041 tatttaatta aaataaaata gtaaaatata aata
//
LOCUS       DAMJVM010000046        25766 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.047, whole genome shotgun sequence.
ACCESSION   DAMJVM010000046 DAMJVM010000000
VERSION     DAMJVM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25766
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.047"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(268..444)
                     /locus_tag="PC523_002069"
     CDS             complement(268..444)
                     /locus_tag="PC523_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475592.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(497..593)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1387..1788
                     /locus_tag="PC523_002070"
     CDS             1387..1788
                     /locus_tag="PC523_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HDF2475593.1"
                     /translation="MNYKNRKRAYYQENSCLDNFICKNCGCIVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNP
                     VKLMSIALKPLAQPPFPLERITEKMEKEETE"
     gene            1785..2852
                     /gene="rsgA"
                     /locus_tag="PC523_002071"
     CDS             1785..2852
                     /gene="rsgA"
                     /locus_tag="PC523_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF2475594.1"
                     /translation="MNGMKEIDLQDYGLNSNFITQSMKYHDLYIARIISQEKNLYQAV
                     CTGGIVFAEVSGKLRFEATIPSDFPTVGDFVILDRCNDKNGNAIIHHVLHRKSLFVRK
                     AAGKANEEQVVAANIDIVFLCMSLNKDFNTHRLERYLSLTWESGAVPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSAKESDGCQQISKYIKPGYTIALLGSSGVGKSSLINC
                     LLGGEFLNTKEIRIADDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGVDKS
                     FADIEAYFGKCHFRNCSHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKTIRR"
     gene            3395..4093
                     /locus_tag="PC523_002072"
     CDS             3395..4093
                     /locus_tag="PC523_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475595.1"
                     /translation="MKISSQYRSQYSFRHESNINNTRINESIVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKDRQEVYTKKINEAIKNLYKSEIRETTNNFSNIEIGIKNSIIED
                     KNKSTMLYENSTYLNTSDEKESLITKEFNEKVEEEMLNDEKLEELEQKKDYKEDSNKK
                     EKISEDLSLAGKTREELENMLKNFTNLTQEEIMKLESRIEKLDKDAEEYKQNSKTNIF
                     DKTDEQKKHINVLL"
     gene            complement(4672..5628)
                     /locus_tag="PC523_002073"
     CDS             complement(4672..5628)
                     /locus_tag="PC523_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2475596.1"
                     /translation="MKNNTTQKRLRDLSKKIWNIYILNPEEEGIKFIISLLDDNLTLI
                     GTGKHEFYETLEEFIIGLEKDQVESNQIDFEIIDEWYSCTKVTDETFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVKDDIIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHI
                     RGDEILIHFSNILTKVFISDAIIGRLGGDEFAVFEYSNQSKEDVENKAKKVLELCLEN
                     NSCSIGISRLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            6331..7326
                     /locus_tag="PC523_002074"
     CDS             6331..7326
                     /locus_tag="PC523_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2475597.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFIGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAIV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSEYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAVAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEELFNHFQKYKKITVSFSNETNIVFSKDCKVVEQT
                     NHKTSVVVPKERGNETIKWIFDRYDVKDVMIEEEDIVNIVERIYKKGSDEYYEQL"
     gene            7304..8167
                     /locus_tag="PC523_002075"
     CDS             7304..8167
                     /locus_tag="PC523_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF2475598.1"
                     /translation="MSIMSNYNEASSNSSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMMFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLSGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKINFAFT
                     SNPIQILISIFMIFLGLIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPL
                     VLLPETIVKVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            8164..8958
                     /locus_tag="PC523_002076"
     CDS             8164..8958
                     /locus_tag="PC523_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF2475599.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPIDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVIIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFSFTLFMWRFWKLGLRHYKSASS"
     gene            9241..10620
                     /locus_tag="PC523_002077"
     CDS             9241..10620
                     /locus_tag="PC523_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2475600.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCHSYIFIILIFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTISGMVFFSKNKGVLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLISEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNMLNINGIWLAVPSAEILTLFLTVFCIC
                     KHKNRYKYM"
     gene            11070..11783
                     /locus_tag="PC523_002078"
     CDS             11070..11783
                     /locus_tag="PC523_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2475601.1"
                     /translation="MDTYFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIVFRVKAQLRRQQYQSIVPSDSSVIKIGDITIDIEGNRVYKDRKEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(12060..13598)
                     /locus_tag="PC523_002079"
     CDS             complement(12060..13598)
                     /locus_tag="PC523_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2475602.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVIPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQESAISGNEQHLIKASDYQEQLIANNFYSIDNEVNKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHEVLLLDEPTNFLDKEHVEWLSNYLNTFSGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKLHLREDYIRRYQSQQKKIEKEEAFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPGFTGKPNIKFSEIEISAQNALTVTNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPCISGDFQFSEQVKLAYYEQDL
                     KWENPTKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLFPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            14606..14892
                     /locus_tag="PC523_002080"
                     /pseudo
     CDS             14606..14892
                     /locus_tag="PC523_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            14880..15206
                     /locus_tag="PC523_002081"
     CDS             14880..15206
                     /locus_tag="PC523_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HDF2475603.1"
                     /translation="MSSYLNLIREFNLKEKIIAFICVSALLIGKSCVLKNRKATTYHL
                     KNSKRQRQLSEFDVNVVNEPTVVNQNIITSYYTETAPHVAFKLLEMLISKKQLDEVKL
                     AMGFKL"
     gene            complement(15211..15534)
                     /locus_tag="PC523_002082"
     CDS             complement(15211..15534)
                     /locus_tag="PC523_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2475604.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLEKL"
     gene            15686..16204
                     /locus_tag="PC523_002083"
     CDS             15686..16204
                     /locus_tag="PC523_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2475605.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLDKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGLNKLSKAANIYDAPLAILVCGDKEKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKVADSPDRHEKTR
                     VPLSEIVSYEIL"
     gene            complement(16395..17063)
                     /locus_tag="PC523_002084"
     CDS             complement(16395..17063)
                     /locus_tag="PC523_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475606.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLILKNSTIKN
                     IIEKHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKIELPLKIDLANGSEHFYGIGAKEQILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKYYISSAFPDLKINS
                     IDIF"
     gene            17178..18593
                     /locus_tag="PC523_002085"
     CDS             17178..18593
                     /locus_tag="PC523_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2475607.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILNGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNYIFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRAEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGVKVDDKGINTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKYATENGYYII
                     EDDYDSEFRYEGQPISSLYELNPNKVIYIGSFSKILSPAIRVGFILLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIEKILEYDHEIILGYAHLS
                     FDEIEKGVKILSKIIHINQGT"
     gene            18816..19274
                     /locus_tag="PC523_002086"
     CDS             18816..19274
                     /locus_tag="PC523_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2475608.1"
                     /translation="MEIIDYIEENLTENLNLDKIADIANYSKYHLHRMFTNIVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITAGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKYISYANMNCKQRIDEINSYLNFNINCEVKVA"
     gene            19271..19855
                     /gene="clpP"
                     /locus_tag="PC523_002087"
     CDS             19271..19855
                     /gene="clpP"
                     /locus_tag="PC523_002087"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDF2475609.1"
                     /translation="MSLVPHVIEQTSQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSVTAGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRYALPNAEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDRIFSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            20158..21234
                     /locus_tag="PC523_002088"
     CDS             20158..21234
                     /locus_tag="PC523_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2475610.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGSPTLSYYRERLFKRSSNHKWVDPIHEVVPLSGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKVLEPRHQFYYSRELYYNAKYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPYIQLSVCYDRLGESNKAIYYHEK
                     TKEIKPNDSAVLHNENYFNKFKNS"
     gene            21301..23349
                     /locus_tag="PC523_002089"
     CDS             21301..23349
                     /locus_tag="PC523_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore surface glycoprotein BclB"
                     /protein_id="HDF2475611.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNSGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGGTGRGGPT
                     GPTGPTGPTGPTGPIGFGITGPTGATGATGATGADGVTGPTGPTGATGANGITGPTGA
                     TGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGVTGPTGAVGATGIGITGPT
                     GPTGATGADGATGATGPTGATGTTGADGVTGPTGATGATGFGVTGPTGPTGATGVGVT
                     GATGLIGPTGPTGATGLIGPTGVTGATGADGVTGPTGPTGATGADGATGVTGPTGATG
                     ATGADGLVGPTGATGATGADGVTGPTGATGATGVGVTGATGATGATGPTGADGLVGPT
                     GATGNTGADGVAGPTGATGATGNTGADGATGPTGATGNTGADGATGPTGPTGATGVAG
                     VTGATGPTGATGATGADGATGPTGATGATGADGLVGPTGATGATGITGATGVTGPTGP
                     TGASAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGVSIVGGVIDLTNAAGTLTN
                     FAFSMPRAGTITSISAYFSTTAALSIVGSTITITATLYQSTAPNNSFTAVPGATVTLA
                     PPLTGILSVGSISSGIVTGLNIAATAETRFLLVFTATASGLSLVNTVEGYASAGISIN
                     "
     gene            23518..24279
                     /locus_tag="PC523_002090"
     CDS             23518..24279
                     /locus_tag="PC523_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HDF2475612.1"
                     /translation="MKNKIETLMNNFVKDYHKFNSITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLKNGKTIITYFIPFDESINNSNIGGFECSEIWARAYIETNMLIS
                     NLNSYLCEELEKIGFDSYAIPGSYNFDSEKLVSDWSQRHVAFISGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDRFDRNKCY
                     EMCTINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            24584..24862
                     /locus_tag="PC523_002091"
     CDS             24584..24862
                     /locus_tag="PC523_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475613.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGEYIYIPRKSNNKKEWGSNTS
                     TREELAIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            25157..25732
                     /locus_tag="PC523_002092"
     CDS             25157..25732
                     /locus_tag="PC523_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HDF2475614.1"
                     /translation="MQKITWEVEYTSQLPVIRYCKKCGKKTEYVCSGLFRVNAQHKYL
                     DIWLIYKCLNCDSTWNSTIYSRVNPNNLSPEVLEQFHTNDDNLANQYAMNIELLHRNG
                     AEVGIPEYKIVGQEIDINDLTQIRIVSKYPSQLKVSTLLREKLGLSRKVFDEMLDCGT
                     IKSVSGVDLRRSKLNYEIVLEVGKEKQKSSN"
BASE COUNT         9557 a         3126 c         4245 g         8838 t
ORIGIN      
        1 aatttataat tttgttaatt taatccttca aacttaaaaa tcatttatgt ttacagaatt
       61 tcattttaag agatatttat tttcatatag ataagaaaat ttaagttatc atatttcttt
      121 tttgatacaa atttacatct ttattacatg tattttaaat aaaaaataaa actatgaata
      181 ctagaaatct attttaatta gttttctaac ataagactat ttaaagtaga taaaactaaa
      241 aagggttctc tagaaaacca actctattta attgtactta cttactaata ccatcacttg
      301 atgcagacaa gattctacta cattttctcc gtactctagt ataaaatcat ctagttgtgt
      361 atctataaaa tcacaataat catatgaact accaatataa gcagtcaaca tatgtgtaca
      421 tctatccatt ttatctctaa acattatatt attatccccc taaaaaactt tcataaaaac
      481 aaaaagaata tatctttttg gcaactggct gacataactc atattttatt ttataagcgt
      541 tagtccctat agctttgcgt cactaaattt ctctagtttt gccgatttag ttttattcta
      601 caattacaat aatacaatag aattgattat tattcaatat acttgtttga aaattaaata
      661 aatttataaa tatgaacgtt attttatata aaaaaatata ctattcatgt aagaatatat
      721 ggaagagctt gattatacgt tttgtttaaa agacctttgt tattaactta aattagataa
      781 attgagggag agatataaga tgataaaaga aataatagcg gttaattttt atgattaata
      841 ttgaatgaat attgaatttc aaaagattac tagattctat agaataaaaa aataagataa
      901 acataaatat atgaatatgt taaattatac ttgaacatac tcatatttta tgataaaatt
      961 tcaataataa ttattagtaa aggagtgtct tgcctggtgt cagagttgta tataatggta
     1021 accaacaaat aaatagttgg aggcgcatat gggttctgtt taatcagaat ttttaagtgt
     1081 atttttgata catttaattg ttgtgtctta ctctgccttt atagataact aacattcgac
     1141 aacaaataat aatattacat attactttgt taaattgtgg tgtagtgggc tttaatctgc
     1201 tacactttta ttaattttac atagtattat tttgaatata gttatgttga aatagaagat
     1261 attaaatttt ctagtccaca ggagttttct gtttttgtgt aggcaaatat atataccaca
     1321 caaaaaggca gatataaata cctgtggatt tttttgtata aaacacaaga tatggaggcg
     1381 ataaaaatga attataaaaa tagaaaacga gcttattatc aggaaaattc ctgtttggat
     1441 aactttattt gcaaaaactg tggttgcatt gtagtacctg aaggagctgg tacacaacat
     1501 aggaatcatt gtccacattg cttacatagt cttcatgtgg atatcattcc tggtgaccgt
     1561 gctgcagatt gtgatgctaa tatggaacct attggagtgt gggtgagaaa aaatggcgaa
     1621 tgggcaatta ttcatcgttg tacacgatgt ggacatctta gttctaaccg tattgcagct
     1681 gatgataatc cagtgaaatt aatgtcaatt gcattaaaac cactggcaca accacctttc
     1741 ccactagaga gaatcactga aaagatggag aaggaggaaa cagaatgaat ggtatgaaag
     1801 agattgattt acaagattat ggtctaaata gtaattttat tactcagagt atgaaatatc
     1861 atgatttgta cattgctcgc attatatcac aggaaaagaa cctttatcaa gctgtatgta
     1921 caggtggtat agtttttgca gaggtttcag gaaaactacg ttttgaagct actataccat
     1981 ctgattttcc aacagttggt gattttgtta tacttgaccg ttgtaatgat aaaaatggta
     2041 atgctattat tcatcatgtg cttcatagaa aaagcttgtt tgtgcgtaag gctgctggaa
     2101 aggcaaatga ggaacaggta gttgcagcta acattgacat tgtttttttg tgtatgtcac
     2161 tgaataaaga ttttaacact catcgattag agagatattt gtctttaaca tgggaaagtg
     2221 gagctgtccc ggtagtagta cttaccaaat cggacttatg tgacaatgta catcgtaaat
     2281 tacaagtagt taattctata gccttgggtg tagatgtagt tgttacatca gctaaagagt
     2341 cagatggttg tcaacaaatt tctaagtata tcaaaccagg ttatactatt gctctgcttg
     2401 gttcttcagg agttggaaaa tcttcattaa ttaattgtct gctgggtgga gaatttttaa
     2461 atacaaagga aattcgcata gctgatgata aaggaagaca tgccacaact agacgagagt
     2521 tatttttaat ttcaggtggt ggtattgtta ttgacacacc aggtatgaga gagttgggaa
     2581 tgtgggatgt ttcggaaggt gtagataaaa gttttgcaga tatagaagct tatttcggaa
     2641 aatgtcattt tcgtaattgt tctcatactt cagaaccagg ctgtgctatt tgggaagcta
     2701 ttgctcatca agaactttcg cctaaaagat gggaatccta tcgtaggctt aaaactgagg
     2761 cagtatttgc agacaataaa agcgaatact tagcaaaaaa agaaaagaaa tttaaaaata
     2821 tctcaaaaat aaataagacg attagaagat gagtgtagtt tttttgttct gttcgttcta
     2881 tgactctata tattattaat atattagatt tgccatcaat ttattacttt aatgtgattt
     2941 tgatggcaaa tttgatttat ggtattattt aaggcaattt attaattttt ttaataaagt
     3001 ttgacttttt atataatgat atatacatat gataatgtat acatgtttgt tatgataggt
     3061 tcttttataa agagttctta tgtgagataa gaagaggcat atttatacta atacataata
     3121 gagtatatgc ttatgtatga tagagatgtt tcttaaaagc aaaactgtaa tattgataat
     3181 ttctaaagta aaaatatata aattataaaa tataaataag atatttataa aaattattgt
     3241 tttaatttat aataaaaatt ttttgttgaa aataatgctc gaacctatag aactattaac
     3301 taatatttgt aaatgtatac ttttgatgat aaaatataat taagttaagt tggcttttta
     3361 taaaacataa atttgtttta gagaggtgaa ataaatgaaa atatctagtc aatatagaag
     3421 tcaatattca tttagacatg aaagtaatat aaataataca agaataaatg aaagtatagt
     3481 taagaaaaat gaaactgtag gaaaagacac ttatttatct aatattatga aacaaaagca
     3541 agaacttaat gatagaatta gagatttaaa agatagacaa gaggtttata ctaaaaaaat
     3601 aaacgaagca attaagaact tatataaatc agaaataaga gaaacaacta ataacttttc
     3661 taatatagaa ataggtatta aaaatagcat tatagaagat aaaaataaaa gtacaatgtt
     3721 atatgaaaat tcaacttatc taaatacaag tgatgaaaaa gaatctttaa tcactaaaga
     3781 gtttaatgaa aaagttgaag aagaaatgtt aaatgatgaa aaattagaag agttagaaca
     3841 aaaaaaggat tataaagagg attctaataa aaaagagaag atatctgagg acttatcctt
     3901 agcaggtaaa actcgtgaag agcttgaaaa tatgcttaaa aattttacaa atttaacaca
     3961 agaagaaata atgaaacttg agtcgagaat agaaaagtta gataaagatg ctgaagaata
     4021 caaacaaaat tcaaagacta atatatttga taaaacagat gaacaaaaaa aacatataaa
     4081 tgtactgctt taaacaatat gttttaataa ataagtttat tatattatga aaacataaag
     4141 gtacaatttc aatacacttc tatttttggt ggaacattag attttgatta ccttaagtta
     4201 aaggatgcga cgagagagat gactacttac tatgatgcta aggcattgtc tatattagga
     4261 tttaatcaat aaaaatatgg aaggcttctt gtggaatctt ttttttatta atttgaaatt
     4321 taataaggaa ttttatatta aatttaatca ataaaagtaa aaaaggctat ttatagagcc
     4381 tttttaagta tttttttagt agaatttttt atatcagcat cagttttaat caaaaaatat
     4441 aatattttgg tgttattttt aaaaataaaa ttattaaaat ataatattaa tcatgttaat
     4501 aatattttta gtttataata ttctataata gtgggtatga tgactattgt agctctatta
     4561 ataatagttg gtttatataa atatatgcaa agataggttg ttgtttacct tacctattag
     4621 tatgtaaaac aagatactct gagaaatttg cctattttaa tatcttttgc actaaaacca
     4681 actaaagttt cctttattat ttcgttttga ataatataaa gcataatccg attgtttgta
     4741 taaagattgg aaagtatcag agttattttc taatctagat atacctatag aacaagaatt
     4801 attctctaag cacaattcta aaactttttt tgctttattt tcaacatcct cttttgattg
     4861 attactatat tcaaaaacag caaattcatc tccaccaagt cttccaataa tagcatcact
     4921 tatgaagact ttagtcaaaa tatttgaaaa gtgtattagt atttcatctc ctcgaatatg
     4981 accaaagata tcatttacct gtttaaaatt atctaaatca aagatataaa agtatccgtg
     5041 tgaaacggtt ttaagcattt cttttatgtg tacttcaaaa aaatgtctgt tgtatagtcc
     5101 agtcataagg tctttttctg attttagttt aaattcattt gccattgcta gtgcttcttg
     5161 tgctttttca gttattgttt tagggtaata ctcatctttc atttggtcaa agtttggaat
     5221 agaatggtga acatggataa gtttaattat atcatcttta actctgtata ccatagtaaa
     5281 gcgagtatcc atttctacat agatatcttt ttctaggtaa tctttttcta ttacccaaag
     5341 agtaccatat actataaaag tttcatctgt gactttggta catgaatacc actcatctat
     5401 aatttcaaag tctatttgat ttgattctac ttggtctttt tcaagaccta taataaattc
     5461 ttctaatgtt tcgtaaaatt catgttttcc tgttccaatt aatgtaaggt tgtcatctaa
     5521 caaagaaata ataaatttaa tgccttcttc ttctggattt aaaatataaa tattccaaat
     5581 tttttttgat aaatctctta atcttttttg tgttgtatta tttttcatag attaccccct
     5641 taagatttac agtttatata ttaatgtatt ataagttgaa cccttattac aatcttatta
     5701 taatatagaa ctgaagaaat tacatcctgt ataggtaaaa agtatatctt ttatgataaa
     5761 aagattaaag aaaaataaca agttttaata tgcatattat attcttaatt tataaatgaa
     5821 tattataaat gaaatttaaa ttcaaccctg aggaaataaa ctaaatatat tagtatatga
     5881 atctatactt gaacataaat aacctttatg atatgatgtt gataataata ttatgagagg
     5941 agtgccttga tgtcggagtt gtataatggt aaccaacaat taaatagttg gagacacata
     6001 tattctgtat aatcagaaat tttaagtgta tttttaatac atttaatttg tgtgtcttat
     6061 tctgccttta tagataactg acatatgaca acaaatagta tttaaaatat atattacttt
     6121 gttaaattat ggtgtagtag gttttaatct gctacacttt tattaatttt gtatagtatt
     6181 attttgataa ctatgttgaa atagagagca ttaacttttc tagtccacag gagttttctg
     6241 tttttgtgct gttaaatata agcatagaaa aaggcagata tatacctgtg gattttttta
     6301 tataaattat gaaatatgga ggcgattaaa atgagcataa ttgaacttaa tgaactgact
     6361 aaatcttata gaagatttga gaaaaaggca ggatttatag ggagtgtaaa gtctttattt
     6421 aataggaaat atacagaaaa aacagctata tcatcatttg atttaaagat agaagaagga
     6481 gaatttgttg gattaatagg tcaaaatgga gctggaaaaa ctactcttat taagatgtta
     6541 actggtatta ttgcaccatc ttctggagaa atatctgtta tgggttatta ccctaataaa
     6601 ttagaaaata attttaaaaa acaatatgct attgttatgg gtcaaaaaag tcaactattc
     6661 tttgaattga caccagcaga tacgtttctt ttatttaaag aattatatga tatatctaat
     6721 gaagaatata aaaaaaattt ggattacttt atcgaattat ttgaggtatc tgaatattta
     6781 aatgttcaag ttaggacact ttccctagga gaaagaatga aaatggaact aatagtagcc
     6841 cttctacata atcccaaaat attatttttg gatgaaccaa ctataggttt agatgctgta
     6901 gcccaaaaac aaattaggta ttttctaaaa gaggtaaatg aaacaaaagg aactactatt
     6961 attcttactt ctcattatat ggaagatatt aagagtctct gtagtagaag tattgttgta
     7021 gaaaatagcc gtaaaatata tgatggctct acagaggaac tatttaatca ttttcaaaaa
     7081 tataaaaaaa taacagttag tttttccaat gaaactaaca ttgtattttc aaaagattgt
     7141 aaagttgttg aacaaacaaa tcataagacc tcagtagttg tgccaaaaga aagaggaaat
     7201 gaaacgatta aatggatttt tgatagatat gatgttaaag atgtaatgat tgaagaagaa
     7261 gatatagtta atattgtaga gagaatatat aagaaaggaa gtgatgagta ttatgagcaa
     7321 ctataatgaa gcaagttcaa attcttcaat gttttataaa tttaaacaga gtattagaag
     7381 gtattatgaa gtagccaaaa ttaactttaa agtgcaactt atctggagat ttgatgtggt
     7441 agttaatatg atgtttacga ttgtaaaaat tttatttgct tatattttat ggaaagcaat
     7501 ttttggcgaa aataaaatag tctctggttt tacatttaac tctatgttgt cttactatat
     7561 aataagctca tttttatcag gtattgatat gtcgaagagt accagtgaag agatatgtaa
     7621 tcgagtaaaa ttaggcacat tttcaaagta catgattatt ccaattaatg ttcaaaatta
     7681 ttttattgct aaaaattttg gaatgacatg tttttatttt gtatttaata tgatttctgc
     7741 atttgtatgg atttttatat ttaaaataaa ttttgccttt acatcaaatc caattcaaat
     7801 attaatttca atttttatga tttttctagg acttattttt atgatgaaac tccacttctt
     7861 tattgggatt ttgtcattta aatttcttga gattagtttt tttagaatga taatagaaaa
     7921 tattgttgaa tttattacag gaacaatgat accacttgtg cttttaccag aaactatagt
     7981 aaaagttatg aaggtatttc cattttatta tacaacatat ttgccatcta tgttactaat
     8041 aggtagaaac agcaatgaag caatacaagg attaattatt ttagcattat gggttttatt
     8101 cttttctgta gtaaataaag ttatgtataa aagaatgaga acattatttg atggggtggg
     8161 tatatgatga aaaaaaattt gaaaataata tttatgttat taaaaatgaa attatcaaag
     8221 atgatggtat ttagatttga tttttttgga gcattttttg tagatagttc attatttata
     8281 ttacaacttc taatgtttaa tagtatatat tctcatgtag actcaatagg gggatggcaa
     8341 caaggagaga tgttaatatt tataggaact ttctcattaa taaatgcgat aaatatgacg
     8401 attttctttt ttggtattta tgatattcca cgaaaaattc aggaaggaga attagactac
     8461 tatattacaa aaccaataga cccactattt aggttaacat ttgaaaatat taatattggt
     8521 tcatcaccat taattttagc aagtatactt attgttttat atggaatctc tgaattaaac
     8581 tcagagataa ctttagtcat aataataata tatattttca tggtgctatt gatgacatta
     8641 cttttttatg atgtatgtat tatacttaga acagtttcat tttttgttat atcatcttct
     8701 gcgataatga gattagaaga taatttattg ccaatgaata tgaaaattcc tggtgtaata
     8761 tatgaaggtg tttttaaagt attattctat ttaatcttac catatggaat tatgtccaca
     8821 attccaactc aaattttggc tggaacaata actattaatg gattaatata ttcaatttta
     8881 attgtatttt cgtttacatt gtttatgtgg agattttgga agttaggtct gagacattat
     8941 aaaagtgcaa gtagttaata aattggcgaa aatagacaat ataattatat ataaatacgt
     9001 ttcacatgac actagactat gtaaacgagt atttacacta aaggcattga aagctataaa
     9061 atgattaaag taaggattta aaagttactt tagtataaat ataattgtgt aaaaaagcaa
     9121 gattaattaa aactatatcc aaataattag atataatttt tatgcagaaa ggaggtctgt
     9181 aaatgtctaa ttgtttggaa tatattttaa aaataattaa tgtacagaaa aataaacaaa
     9241 atgagtgatg tatattattc agaaaatcat gaacaagata atctttatga gcaagataat
     9301 ttactggcac aaagatttaa tttatttact ctattgaagt ttgcatttcc tactattttt
     9361 atgatgattt ttatgggatt gtatacaatc gtcgatacta tatttgtatc aagatttatt
     9421 aatacaaatg ctctatcagc aattaatatt gtatgtccaa ttattaatct tatagttgga
     9481 attggaacta tgcttgccac tggaggaagt gctattgtag cacgtaagat gggaaatggt
     9541 gatattgagg gagcaaataa agctttctct ttaatcgtat tatcaggagt tgtaattggg
     9601 gtaataatta ctggtatagg tattgtattt atggattata tcattatatg gttaggagca
     9661 agtgaagttt taattaaata ttgtcattca tatattttta ttatcttgat ttttgcttct
     9721 gcgaatatac tacaagtatt atttcaatgt ttatttgtaa cagctggaaa gcctcaattt
     9781 ggattatggc tagtgttagg tgcagggtgt gctaatataa ttcttgacta tgtgtttatt
     9841 gttctgttac aaatgggtat tagtggagca gcacttgcaa ctggagtagg atacctaatt
     9901 cctaccattt caggtatggt tttcttttca aaaaataaag gggtacttaa attttgtata
     9961 cccagactag ataaaagaat attatttgaa gcttgtttta atggttcatc tgaaatggtg
    10021 ggtcaactat caacagcagt tactactttt ttatttaatg ctatgatgat gaaattaatt
    10081 agtgaagatg gagttgcagc tattacaata attatttatt ctcaatttct tttaacttcg
    10141 ctttatatag gtttctcaat gggtgttgca ccaataataa gttataatta tggaagtgaa
    10201 aataatacac agttaaatca tgtttttaag atttgtatat tatttatttc agtggcatct
    10261 ttattggtat ttatcttttc tatatcatgt tcaccaatca ttgtagagat tttttctcaa
    10321 agaggaagcc gagtttatga aattgcaagg tctggatttt atatattttc ttttagtttt
    10381 attttttgtg gatataatat ttttacctca tctatgttta cagcattgtc aaatggcaag
    10441 gtgtctgcaa tattatcttt tctacgtaca tttggattta taactatagg tttattgata
    10501 ttgccaaata tgttaaatat aaatgggata tggttagcag taccaagtgc tgaaatttta
    10561 actttatttt taaccgtttt ttgtatatgt aagcataaga atagatataa atatatgtag
    10621 atattaaata aatagtttag gtagatattt tatgttttaa taagtaattc aattagtggt
    10681 agatagatac tattgaaaat taaagttata attgatataa agaattaata aaaataacat
    10741 ataaaagttt aaaaggggag ttgaacttaa agtttgatta gaaaaagtcg attttaagac
    10801 aactcctttt attttactat tgtattaatc tgtatctgta agtgtattag tctaatatta
    10861 taaactttgg atgaaattaa ttttctatag gttttaatgt atcatatact ttattttaag
    10921 gaaacgtatc ttaattttca tagtgattga atttgtatat tagagatgtt actattagta
    10981 atgtaaaagc aagagtagtg aatataaaaa aacaagatag tgatactgca agattagaat
    11041 ataataaatt tttatggaga gatttcaata tggatacata ttttaataaa aaaatattac
    11101 ttgttgatga tgaaaaagat atagtagatt taattgaaga agtacttata aatgatggtt
    11161 ttaaaaacat tattaaagct tataatggtt tagatgctat ttcgctgtgt aagatagcat
    11221 gtccagatgt tgtcattctt gatataatgt taccagatat agatggcata gaagtgtgta
    11281 aaaaaattcg agagttttca tactgttcta ttctttttct ctcatcaaaa aatgatgata
    11341 ttgacaaaat actaggtctt agtagtgggg gagatgacta tataacaaag ccattcagtc
    11401 caagagaaat tgtttttcga gttaaagcgc aattacgtag acaacagtat caaagtattg
    11461 taccatcaga tagttcagtt attaagatag gagatattac tattgatata gaaggaaacc
    11521 gtgtatataa agatagaaaa gagattgagt taactggaag ggagtatcat cttttaagct
    11581 acatggctca aaatgtaaat aaaattatag gaaaagaaag actctatgaa caagtatggg
    11641 gagtatacag tagtatttgt gacaatacaa ttatggtgca tatacgacat ataagagaaa
    11701 aaattgaaga taacccctca aatcctaaaa tacttataac agttaaaggt ctaggatata
    11761 aacttgtgaa tagaatcgat tagaatagat cttagtatca atgaaggtgt ctcaaattca
    11821 atttgttagt taatgagaca ttcttttttg tgtgaaatca aatgtatttt tgttatatca
    11881 ataatgaaga agaggcttat ctctattttg agatagcctc attaaattta taccgtggta
    11941 atgcacttta tgctctaata aatatttatt ttttagttta cttgcagtga aacattaact
    12001 aggagattaa aatttgcatt gaaatgctaa ttaaacaata tcgcatattg tatataaacc
    12061 tacactaatt gtttttctat attaaatact ttgtcaatcc agcctttata aaatttttct
    12121 tcatgggaaa ctagaattat acttcctcta aactgtttca aagcattttg taaagcatcc
    12181 tttgtttctg catcaaagtg gttagttggc tcatctaaaa tcaaaaaatt acaagggaat
    12241 aacatcatac aacataattt taccttagat tgttcaccac cacttaatgt agatacactc
    12301 cttgaaacat gttcttcctt tacgccacat ctggctagat gacgtctaat ctcttttgta
    12361 ttcaatttcg gatatttatc tgctactatt tgaagcggtg ttttagtagg attttcccat
    12421 tttaaatctt gctcataata agcaagtttc acttgttcag aaaactggaa atctccagaa
    12481 atacaaggaa tatctttaac taatgttttt aacaaagtag atttaccaat tccattaaaa
    12541 cccgttataa ctattttttg acccccatca acagagaaat ttaattttgg aagcaaagaa
    12601 tagtagtagc caacttcaag atttgttact gtaagagcat tttgagcaga tatttcaatt
    12661 tctgaaaatt ttatatttgg ttttcctgta aaaccaggag gagctattct ttctatttta
    12721 tccaattgtt tttgtcttcc tcttgcaatt tttgaattta ctccagcttt gtttttacgt
    12781 ataaatgcct cttctttttc tattttcttt tgttgtgatt gatatcttct aatataatcc
    12841 tctcttaaat gtaatttttg tttaaggaac tctgaatatt ttccatgata tttcttaatc
    12901 ataccaaatt ctatatcaag aatacctgta gaaattttct ctaaaaagtc aaaatcgtga
    12961 gaaactacaa taaatgctcc actaaatgta tttaaataat ttgagagcca ttcaacgtgt
    13021 tctttatcta aaaagtttgt tggttcgtct aataataaaa cctcatggtt agaaagtagc
    13081 aattttgcta aaattacctt tgccctttgc ccaccgctaa gctccccaac aacacgattc
    13141 attcctattg aatctaatcc aagaccattt gctattttat ttacttcatt atctatagaa
    13201 tagaaattat ttgctattaa ttgttcctga taatctgatg ctttgattag atgttgctca
    13261 tttccagata tagcactttc ttgatagagt aaattcatct ccttttctat tttataaagt
    13321 tcttcaaatg cagtgtgtaa atagagtgat attgttgtat ctctattgat ttctgcatat
    13381 tggtctaaat ggcctatatt gatattaggt tgccacttga tttctcctga atctggaata
    13441 acttctccca ataaaatttt aattaatgta cttttgcctg taccattttg gccaactaca
    13501 cctatatgtt cacctttgaa taaatcaaag ttagctttct catacagtga tttatccata
    13561 aaacattgtg ataaattagt tacttttaat aaactcataa ttcaaactcc ttttctttta
    13621 ttaaaaaatt tatggaaata aaaaaagcag aggaatgaca ttataaaatg ccgacctctg
    13681 cttttggaaa aacaaaaggt catagacaag cattgttgtc tatgaccaga aaatctatat
    13741 tatctatcgg caaataggta attttaaata agcatagccc aaaaactaca ctaaacctat
    13801 atttgccgat gaagatagaa agctttaagc tttcagatta acctctacac aacgcttcat
    13861 cgtatacatt tgtgtagagt taagttttct aacaagttta actaattcta tataaatcat
    13921 aattattcac atcctaatct tagtattaat tctagtttat ctatatttaa attgtacttt
    13981 tgtactttgt atattgcctt atttacttaa aatatatttt attaattata gcttaaatca
    14041 tgttatcata atacttagtt atagtcaata agaaagttat atttgctaca taacctatta
    14101 atttagtaaa tctatcatta caaagttatt tgaagactca ccaccaataa aattttataa
    14161 tctattaaaa aattaagatt acgagattta taattttgat acaggtatac tatctttatt
    14221 aaatattgca acatgtagaa gtagatggat aatattatta aattttcaat gatatgctaa
    14281 tgtacatata taattattag aaataaaaat ataatagtat gaaagtaact gatataataa
    14341 ctaaatgatt aaacatatag taaaagtact tcataaatta tggagtatct tttattgtcg
    14401 caaaaataag caatatatta tacaaaataa aaacattaat tcaacttaaa taattttaag
    14461 tgtaatatgt attttgatta aataagctaa aattctaaga aaaaataata tatttgtatt
    14521 aagttttatt gaaataatta aaattattta cttaatgtaa attgttagct taaattatat
    14581 taaataatta aggcggtgag tgggtctgga tgagatagat ttaaaaataa ttaattctct
    14641 aagaaagaat ggtaggtttc aacttctgaa ataagcagaa aaattaattt atcaatacca
    14701 gcagtctcag agcatataag gaaaattgag caattaaatt taattcaaaa gtacactatt
    14761 caaattaatc aggaaaaaat aaaatataag cttttggctt ttgtatttat caatacggat
    14821 ttatctaaaa gtatggaaga atttaagaaa actatattgg aatatgatac tgcattagaa
    14881 tgtcatcata tttaaatcta ataagggagt ttaatttaaa agaaaaaata atcgctttta
    14941 tttgtgtgtc agcattgctg attggaaaaa gttgtgtttt aaaaaatcgt aaagctacta
    15001 catatcattt gaaaaatagt aaaagacaaa gacagttaag tgagtttgat gtaaatgtag
    15061 ttaatgaacc aactgttgta aatcaaaaca ttataacctc atattataca gaaacagctc
    15121 cacatgtagc ttttaagctt ttagaaatgt tgatatctaa aaaacaactg gatgaagtta
    15181 aattagctat gggatttaaa ctgtaatagt ttagagcttt tccaagtaat cagaacccca
    15241 gtcgcacatg gaatctaaaa ttggaataat actctttcca aaatcagata gtgagtattc
    15301 tacttttgga ggaacaacag gataaaccac tcttatgatt aaaccatctg attctaattc
    15361 acgtaattgt tgagttaaca ttttaggtgt tgccttaggg attaattttt ttaattcatt
    15421 gaacctcaat atagtgtctt taagatgcca aagtataagt gtcttatatt taccaccaat
    15481 caatgcaagt gttgcttcta ttgggcaact gtaattatct aaacattttt tcatttttgg
    15541 actcctttat ttattttaaa gtatcttttt aggtactata ttacaaaaaa gtgcattatt
    15601 gtaatatatt accatagtgc taaaattata gcataggtta aataaatcat caacttagtc
    15661 taataaaaca ataggaggta aataaatgaa tttcgttgaa cttgcaaaaa aaagatattc
    15721 ttgccgtaat tatcaagata gaaaggttga aaaagaaaaa cttgataagg ttttagatgt
    15781 tgcaagaata gctccaactg gtggtaatcg tcaacctcaa agattgattg ttattcagga
    15841 aaaagagggt ctaaataaac tttccaaggc tgccaatata tatgatgctc cattagcaat
    15901 tcttgtgtgt ggagataaag agaaggtttg gacaagacct tttgatggga agcaacttac
    15961 agatattgat acaagtatag ttacagacca tatgatgcta caagcaactg aacttggatt
    16021 ggcaagtgtt tgggtatgct attttaatcc agatataatt agagaagagt ttagtttgcc
    16081 agataatcta gaaccaataa atattttatt gatgggatat gaatctaagg tagcagatag
    16141 cccagataga catgaaaaaa ctcgtgttcc tttaagtgaa attgtttctt atgaaattct
    16201 ttaattataa aattaagtta ttcataggtt aaatgtaaca aagtgtttat tataaataaa
    16261 actcaaaata aataatatcc ttagataaat aatatctata taattaattt tagtgaagta
    16321 tatctagttt aactagaata tatacttcac taatttattt gttcaaaata tttgtgagga
    16381 ttttaatata ttaactaaaa tatatcaatt gaattaattt ttaaatctgg aaaagcactt
    16441 gaaatatagt attttatgtc atttaaatgg tcttgaattt gtttttctga tattttatca
    16501 ggtccatata caaaatacat aatattttta gtatcttttg ttatagctgt tcgattatca
    16561 ggtccattaa gaatgctaga taatatacca atattatccg ataaaaataa atcatctttt
    16621 gtcaaaatct gttctttagc acctatacca taaaaatgtt cacttccatt tgctaagtct
    16681 attttcaaag gtaattctat tttatcaagg tcatgacctg ctgttagaag aagatttttc
    16741 aattctgcta aaaacattga ctcaattgtt acacctacat ctgggatacc ttttgatttt
    16801 aatagaatag attctaattg aagcaataca gggtaagttt tcttgaattt tttgtaatac
    16861 tttatataac ttgaaacagg ctctgtttta attttttctt tacgattgta ttctggatgt
    16921 ttttctataa tatttttaat tgtagagttt ttaagaatta aaaaattttc tttattaggt
    16981 tttgaatgtg taatatttat aaccataact ccaaatttaa tattgggata aatttctttc
    17041 actttatgtg atatttctaa cataatatag actccttttt attttttgtt ttatgatata
    17101 atcttagcaa gttaaaagta gattttgtag ttccattttt atagtaattt tatagaacca
    17161 cttttggagg tattagaatg tggattacga ttaatagaaa tagtaatata tcattatcaa
    17221 gacaaattta ttgtgagata aaagatatga ttctgaatgg aagcttaaag tttgagcaaa
    17281 aattgccatc atctaggact ttatcaaaag aactatctgt atctcgtaat acagttttag
    17341 atgcatataa tcaattaatt gcagaaggat atttggaaac aaggaatgga tatggaacaa
    17401 ttgtagctaa aggaatatca gggcataaag ttgattttaa gttaaattat atttttagag
    17461 atgaaaaaga tttacatact aataaacaaa aaaattatat agattttcgt tcaggagttc
    17521 cagaattaaa actatttcca cgcaaagaac tttcaaagct atataaacaa atactgtatg
    17581 atttatctga ttcggatttc agatatggaa gtacagctgg tgtatgggtt ttaagagcgg
    17641 aaatatctaa gtatttattt cgtactagag gaattagatg taatcccaaa aacattatga
    17701 ttgtttctgg ttcaacacaa ggtttatctt taatttcaca gcttttatac aaaagtaata
    17761 aaaaggttgc tgtagaagac cctattcata aaggcttatt aaaggttatt tcatctattg
    17821 gatattcagt tgttggagtt aaagtagatg ataagggaat aaataccaac ttaataaaat
    17881 caataaaggg gatttctttt gtttatacta caccttctca tcaataccca ttaggaggga
    17941 ttttacctat tcaaagaaga ctggagctta ttaaatatgc aacggagaat ggttactata
    18001 taattgaaga tgattatgat agtgaattta gatatgaggg tcaaccaatt agttctcttt
    18061 atgaacttaa cccaaataaa gtaatttaca taggttcttt tagtaaaata ttatctccag
    18121 ccattcgtgt tggatttata ttattaccag acaatatttt agaaagctat aaaaccttaa
    18181 aaatgtactc tgatgttcat acagaaacaa tattacaaca tgtattagct gaatttattg
    18241 gaaatggagg tttagagaag tacatattta gaatgaagaa gttgtataat agaaaacgta
    18301 agtacctcat aaaagtttta aacacggaat ttaaaaatga atttaaaata aaaggtgaag
    18361 cagcagggct tcatatagtt gctcatttct ataatgttaa atttacggaa agtttaattc
    18421 ataaaatttt tgaagcaggc ataaaagttt atcctataca acaatatgca attgagaaaa
    18481 tattagaata tgaccatgaa attatactag gttatgctca tcttagtttt gatgaaattg
    18541 aaaaaggtgt aaaaatactc agcaaaataa tacatataaa tcaaggtaca tagataaaac
    18601 atatagcaat ctacatataa tagatgcaat gaagtttaaa ctttaaaaga aatatatatt
    18661 aatatttttg tataaaaata cctgatattg tgttatcgat actttgttgc tatacactaa
    18721 attaagatag caatttatat taagaaaaaa ctttttgcat aggttataat acttaaaagg
    18781 agaggataga gttgaaaaca aatacagatg ttattatgga gataattgat tatatagaag
    18841 aaaatcttac agaaaatcta aatttagata aaatagctga tattgctaat tattcaaaat
    18901 accatttaca cagaatgttt acaaacattg taggatgtac tattcatcaa tacatccaga
    18961 ggaggagact cactgaagca gctagacagc ttatatttac agacaaatat attatagaca
    19021 tagctattac agcaggatat tctacacatc aatcttttac actagcattt agaaatttat
    19081 ataaagaatc tccacaagta tatagaaata aagatgaatt ttatcctatt caacagagat
    19141 ttgttttaaa tctaaatcat cagattcaaa gcgaaaaata tatttcttat gcaaatatga
    19201 attgtaaaca aagaatagat gaaattaatt cttatcttaa ctttaatatt aattgtgagg
    19261 tgaaagtagc atgagtttag taccacatgt aattgaacaa acaagtcaag gggaaaggtc
    19321 ttatgatata tattcaaaat tattaaaaga tagaattata tttataggag aagaaattaa
    19381 tgatgctatt gcaagtttag tagtagctca attactgttt ttagaatcag aagacccaga
    19441 ttcagatata attatatata taaatagccc tggtggctct gttacagcag ggtttgctat
    19501 atatgatact atgaattatg tcagatgtga tgtatcgact atttgtattg gtatggctgc
    19561 aagtatgagt gcttttttac ttgcaggagg aacacatgga aaaagatatg ctttacctaa
    19621 tgcagagatt atgattcacc agccaatggg aggagctaaa ggtcaagcaa cagatgtaaa
    19681 aatagctgta gataatatat tgaaaataaa agaacgatta gatagaattt tttctgaaaa
    19741 tacaggtaaa tcaatagaag atattagaag agatacagat agagataatt ttatgactgc
    19801 actagaagct aaagagtatg gactgattga ttatataatg aataggaatg aataaagtat
    19861 catattaaaa tatctgtttt ataaatatat atagaatatt aaataagagt gtctcaaagt
    19921 gaacttttta gtttatggga tgctcttttt tgtatgaaat taaatatagt tttattattt
    19981 caataactag tatcaaattt caagtttaat aaattctacc ataaaaatac ataaaagata
    20041 aggtttttta gtaactttct gtcgtcaaaa gtttttataa atttaataac aaaacaagag
    20101 aaaataccat atattactta tgtctgcaat atatagaaga caaaggagtt gtacattatg
    20161 attacaataa gtttgtgcat gattgttaaa aatgaggaag aagttatagg cagatgctta
    20221 gaatgtgtaa aggatatagt agacgaaatt ataattgtag atactggttc tacagattcc
    20281 acaaaagaaa tagtctcaaa atatacagac atggtgtacg atttcgaatg gatagatgat
    20341 ttctctgctg caagaaattt ttcattttct aaagcttcta aagactatac gatgtggctt
    20401 gatgcagatg acataatttt agaagctgat agagaaaaat tattaaagat taaagaaagc
    20461 ttggatactt ccattgatat tgttatggca aaatacaatg tatcctttga tgaaaatggt
    20521 agcccaactc tctcgtatta tagagaacga ttgtttaaaa gaagttctaa tcataaatgg
    20581 gttgacccta ttcatgaagt agtacctctt tctggagagg tattttattc tgacattgca
    20641 atatctcata aaaagcttca tagacaagac cctctaagaa atcttagaat ttttgaaaaa
    20701 atgatttcag aaggaaaagt attggagcca agacaccaat tttattactc tagagagttg
    20761 tactataatg ctaagtataa agaagcaata gaaggtttta ctaagttttt agactcatca
    20821 agaggatgga ttgaggattg tatcagtgct tgcagagatt tagccacatg ttattactta
    20881 attaatgatg aaaagagtgc cttatattct ttgtttagaa gttttgaatt tgatgagcca
    20941 agagcagaaa tttgttgtga cataggaaag catatgtttg atagacaaaa atataaagaa
    21001 gctatttttt ggtataaggt agcactaaca agagataaga atgatactaa tggaggtttt
    21061 aagtcaaatg attgttatgg atatatacca tatatacaat taagtgtctg ttatgacagg
    21121 ctaggagaat caaataaagc tatttattat catgaaaaaa caaaagaaat caaacctaat
    21181 gatagtgctg ttttgcacaa tgaaaattat tttaataaat ttaaaaatag ttgataggtc
    21241 acaataatac taaatttgac atattatata tagagcttag aagctcaaat atatcaggag
    21301 gtgcttttaa taatgagtag aaataaatat tttggaccat ttgatgataa tgattacaac
    21361 aatggctatg ataaatacga tgattgtaac agtggtcgtg atgattataa tagctgtgat
    21421 tgtcatcatt gctgtccgcc atcatgtgta ggtccaacag gtccaatggg tccaagaggt
    21481 agaacaggcc caacaggccc aacgggtcca acaggaccag gagtaggtgc aacgggtcca
    21541 acaggtccaa caggcccaac aggtccaaca ggaaatacag gaggaactgg aagaggaggc
    21601 ccaacaggtc caacaggtcc gacgggtcca acaggtccaa caggcccaat aggatttgga
    21661 ataacaggtc caacaggcgc aactggagca acaggtgcaa ccggagcaga tggagtaaca
    21721 ggcccaacag gcccaaccgg agcaaccgga gcaaatggaa taacaggtcc aacaggtgca
    21781 accggagcaa caggatttgg agtgacaggt ccaacaggcc caaccggagc aacaggagta
    21841 ggagtaacag gagcaacagg attaataggc ccaaccggag caacaggaac acctggagta
    21901 acaggtccaa caggggcagt aggagcaaca ggaataggaa taacaggtcc aacaggtcca
    21961 accggagcaa ccggagcaga tggagcaaca ggggcaacgg gcccaacagg agcaacaggg
    22021 acaaccggag cagatggagt aacaggtcca acaggtgcaa ctggagcaac aggatttgga
    22081 gtgacaggtc caaccggccc aaccggagca acaggagtag gagtaacagg agcaacaggg
    22141 ttaataggtc caacaggccc aaccggagca acagggttaa taggtccaac aggagtaaca
    22201 ggggcaactg gagcagatgg agtaacaggc ccaacaggtc caaccggagc aaccggagca
    22261 gatggagcaa ccggagtaac aggtccaaca ggtgcaacag gggcaaccgg agcagatgga
    22321 ttagtaggtc caacaggggc aaccggagca actggagcag atggagtaac aggcccaaca
    22381 ggggcaaccg gagcaaccgg agtaggagta acaggagcaa caggggcaac tggagcaact
    22441 ggcccaaccg gagcagatgg attagtaggt ccaaccggag caacaggaaa taccggagca
    22501 gatggagtag caggtccaac aggagcaaca ggagcaacag gaaatactgg agcagatgga
    22561 gcaacaggtc caacaggagc aacaggaaat accggagcag atggagcaac aggcccaaca
    22621 ggtccaacag gggcaaccgg agtagcagga gtaactgggg caacaggccc aacaggagca
    22681 acaggggcaa caggagcaga tggggcaaca ggcccaacag gagcaacagg ggcaacagga
    22741 gcagatggat tagtaggtcc aacaggggca acaggagcaa caggaataac aggagcaact
    22801 ggagtaacag gcccaacagg tccaacaggt gctagtgcaa taataccttt tgcatcaggt
    22861 ataccactat cacttacaac tatagctgga ggattagtag gtacaccagg atttgttggt
    22921 tttggtagtt cagctccagg ggtaagtata gttggtggag taatagatct tacaaacgca
    22981 gcaggaacat tgactaactt tgcattttca atgccaagag ctggaacaat aacatctatt
    23041 tcagcatact tcagcacaac agcagcactt tcaattgttg gttcaacaat tacaattaca
    23101 gcaacacttt accaatctac tgcaccaaat aactcattta cagctgtacc aggagcgaca
    23161 gttacactag ctccaccact tacaggaata ttatcagttg gttcaatttc tagtggaatt
    23221 gtaacaggat taaatatagc agcaacagca gaaactcgat tcttactagt atttactgca
    23281 acagcttcag gtctttcatt agttaatact gtagaaggat atgcaagtgc aggaatttca
    23341 ataaattaga ttaaataatt aaaaggcttc tagtatctta ttgatattag aggcttttta
    23401 agactgattg ataaacatag ttttaatatt aattagtgag cgtaatatag agtattgtta
    23461 tataatataa aataaaaacg ttaaattaga aatagagaat tatctagggg ggatactgtg
    23521 aaaaacaaga ttgaaacatt aatgaacaac tttgtaaaag attatcataa atttaatagt
    23581 ataactacaa agtggcaaga gcttttgttt gcttatgcat ctgccgagga tgatatgttt
    23641 cagaagttaa aaagtgttgt tggaacatct catgctatgc caaaagattt tctgaaaaat
    23701 ggaaaaacta taattacata ttttatacct tttgatgaaa gtataaacaa tagtaatatt
    23761 ggaggatttg aatgttctga aatatgggca agagcgtata tagaaactaa tatgttgatt
    23821 tcgaatttaa atagttattt atgtgaagaa ctagagaaaa taggatttga ttcatatgct
    23881 attcctgggt catataattt tgatagtgaa aagttggtca gtgattggtc acaaagacac
    23941 gtagcattca tatcaggtct tggaactttt ggtttaaaca atatgcttat tacagaaaaa
    24001 ggatgttgtg gaaggatagg aagtataatc acaagtttag aattagagcc aactaaaaga
    24061 ccagaaaatg aatattgttt atataaacat aataatagtt gcaaaaaatg tgttaataaa
    24121 tgtgtaaaag aagctttaaa agaagataga tttgatagaa ataagtgtta tgaaatgtgt
    24181 acgataaatg aaaagaagta cacttatata ggtggatgtg atatttgtgg aaagtgtata
    24241 gttggagttc cttgttcagt aataaaccca tcaagataat ggatttatgt ttattaataa
    24301 ttttaaatat aatttataat gaaaaaactt tagctatcaa aataaatata aaattaaata
    24361 aaatataatt taaaagttag ataaccaatt tatattgatt gacaattatt tatactgttg
    24421 atagacttat atgtatcgct gtgatatata aggagggttg agactttatg tttgatttaa
    24481 tctttatttt tgcatagcaa gggctattgc aactataaaa tttaatatat aaatagccct
    24541 ttgctattcc taataaaaaa ttactattta ataggagagc aaaatgagct ataaaaaggc
    24601 aatacatata ttaccagaag aattacttga actgatacag gaatatgtag atggtgaata
    24661 tatctatatt cctagaaaat ctaataataa aaaagaatgg ggaagcaata cttctactcg
    24721 tgaagagtta gcaattagaa atatgcagat ttacgaagac tatcaagttg gatatgattt
    24781 gcaatattta tcggaaaagt attatttatc tttgaaaagt atccaaagaa ttgtattaca
    24841 agaaaaaaga aaagatatct aaggatgagc cagtatagtt ttataattat actggctcat
    24901 tttcttttga taaaagtgtt tgaggtatag tttcctataa gtagatgtta taatttaagt
    24961 aatttcaata acttatacaa cagataagca atagagttgt aaagtgcata tataagagat
    25021 aatagctttt tatatgtatg aaattaaaat ttatcaaaaa ctaggaggtc ttgttttgaa
    25081 aaaaatactt gattatattc caccttttca taactttcta ttagtttagt aactttatag
    25141 aaaggaaatt atatttatgc aaaaaattac ttgggaggtg gagtacacct ctcaattacc
    25201 agtaattagg tattgtaaaa aatgtggaaa aaagactgaa tatgtatgtt caggtttatt
    25261 tagagttaat gctcaacata aatatttgga tatatggcta atatataagt gtttaaactg
    25321 tgactcaaca tggaactcaa ctatatattc tcgtgtcaat ccaaataatt taagtccaga
    25381 agtactagaa caattccata caaatgatga taatctcgct aatcaatatg ctatgaatat
    25441 agaactttta catagaaatg gagcagaggt tggtattcca gaatataaaa tagtaggaca
    25501 agaaattgat attaatgacc ttacacaaat tcgtattgta agtaagtatc catctcaatt
    25561 aaaggtatca acattattac gtgaaaaatt aggtttatct aggaaagtat ttgatgaaat
    25621 gttagattgt gggactatta aaagtgtatc tggagttgat ttaaggagaa gtaaattaaa
    25681 ttatgaaatt gtgttagagg taggcaagga gaagcaaaag tcttcaaatt gattaaaaaa
    25741 tagctacgca tactcgtgac tttagt
//
LOCUS       DAMJVM010000047        25639 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.048, whole genome shotgun sequence.
ACCESSION   DAMJVM010000047 DAMJVM010000000
VERSION     DAMJVM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25639
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.048"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            89..970
                     /locus_tag="PC523_002093"
     CDS             89..970
                     /locus_tag="PC523_002093"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2475615.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENDTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPEDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VAKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKHKLTNFGFKF"
     gene            1400..2401
                     /locus_tag="PC523_002094"
     CDS             1400..2401
                     /locus_tag="PC523_002094"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDF2475616.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            2518..3405
                     /locus_tag="PC523_002095"
     CDS             2518..3405
                     /locus_tag="PC523_002095"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2475617.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            3473..4222
                     /locus_tag="PC523_002096"
     CDS             3473..4222
                     /locus_tag="PC523_002096"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDF2475618.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDSELELVCVAARGLDSYEDDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDEEMNKIHEAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            4404..5120
                     /gene="dapD"
                     /locus_tag="PC523_002097"
     CDS             4404..5120
                     /gene="dapD"
                     /locus_tag="PC523_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDF2475619.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     CNGSYIFVGDYEDIMEVLDKHGNDIIDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(5403..6392)
                     /locus_tag="PC523_002098"
     CDS             complement(5403..6392)
                     /locus_tag="PC523_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDF2475620.1"
                     /translation="MLKEEKTYQEKEAKVFNGVAALIMIVLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLIFGLIFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAASRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            6835..7590
                     /locus_tag="PC523_002099"
     CDS             6835..7590
                     /locus_tag="PC523_002099"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDF2475621.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELKLVCGVARSADEKSHHS
                     DTKLYKTMTDVTEKADVIIDFSHHETLEPILSYALKNNTPVVLATTGYDESDLSKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIKEVLPESTNTHGRYGRDCKRKANEIGIHSIRGGNIISDHDVIFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLVGKQSGLYKMSDILG"
     gene            complement(7864..9549)
                     /gene="bclA2"
                     /locus_tag="PC523_002100"
     CDS             complement(7864..9549)
                     /gene="bclA2"
                     /locus_tag="PC523_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HDF2475622.1"
                     /translation="MSDISGPNLYQDVGPTGPTGPTGATGPTGPTGPRGATGATGANG
                     ITGPTGNTGATGANGITGPTGNMGATGPNGATGSTGPTGATGATGANGITGPTGNTGA
                     TGANGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLT
                     GATGATGANGITGPTGNTGATGANGVTGSTGPTGSTGATGPTGAVGATGANGVTGATG
                     PIGATGPTGADGEVGPTGAVGATGPDGVIGPTGPTGATGATGANGLVGPTGPTGATGA
                     NGLVGPTGPTGATGVAGAIGPTGTVGATGPTGADGAVGPTGATGATGANGATGPTGAV
                     GATGATGVAGPIGPTGPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGPIG
                     PTGPTGANGVTGATGNTGATGANGATGPTGATGATGVLAANNAQFTVSSSSLGNNTLV
                     TFNSSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVAQNNF
                     IAKAVNTLTSSDVSVSLSFLVDTRAAAVTLSFTFGSGTTGTSPAGYVSVYRIE"
     gene            complement(9906..10445)
                     /gene="hpt"
                     /locus_tag="PC523_002101"
     CDS             complement(9906..10445)
                     /gene="hpt"
                     /locus_tag="PC523_002101"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDF2475623.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLKELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGKVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(10774..12081)
                     /locus_tag="PC523_002102"
     CDS             complement(10774..12081)
                     /locus_tag="PC523_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDF2475624.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDSAEKVESLMDTVTIQELIKNVEGIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(12505..14280)
                     /locus_tag="PC523_002103"
     CDS             complement(12505..14280)
                     /locus_tag="PC523_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2475625.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKDLFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GVSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDNITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLKERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVRSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(14277..14414)
                     /locus_tag="PC523_002104"
     CDS             complement(14277..14414)
                     /locus_tag="PC523_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475626.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(14492..15061)
                     /locus_tag="PC523_002105"
     CDS             complement(14492..15061)
                     /locus_tag="PC523_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2475627.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(15245..15880)
                     /locus_tag="PC523_002106"
     CDS             complement(15245..15880)
                     /locus_tag="PC523_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2475628.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
     gene            complement(16546..17427)
                     /locus_tag="PC523_002107"
     CDS             complement(16546..17427)
                     /locus_tag="PC523_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDF2475629.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWVNVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
     gene            complement(17547..18554)
                     /locus_tag="PC523_002108"
     CDS             complement(17547..18554)
                     /locus_tag="PC523_002108"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HDF2475630.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            complement(18574..19056)
                     /locus_tag="PC523_002109"
     CDS             complement(18574..19056)
                     /locus_tag="PC523_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HDF2475631.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIM
                     GVDGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            complement(19073..19540)
                     /locus_tag="PC523_002110"
     CDS             complement(19073..19540)
                     /locus_tag="PC523_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HDF2475632.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            complement(19555..20313)
                     /gene="prdD"
                     /locus_tag="PC523_002111"
     CDS             complement(19555..20313)
                     /gene="prdD"
                     /locus_tag="PC523_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HDF2475633.1"
                     /translation="MEEKIIRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKEQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            complement(20393..21118)
                     /gene="prdB"
                     /locus_tag="PC523_002112"
     CDS             complement(20393..21118)
                     /gene="prdB"
                     /locus_tag="PC523_002112"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(20666..20668),aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HDF2475634.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            complement(21156..21440)
                     /locus_tag="PC523_002113"
     CDS             complement(21156..21440)
                     /locus_tag="PC523_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475635.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            complement(21521..23401)
                     /gene="prdA"
                     /locus_tag="PC523_002114"
     CDS             complement(21521..23401)
                     /gene="prdA"
                     /locus_tag="PC523_002114"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HDF2475636.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            complement(23830..25590)
                     /locus_tag="PC523_002115"
     CDS             complement(23830..25590)
                     /locus_tag="PC523_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF2475637.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSGQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISERVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKEVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKINVSKI"
BASE COUNT         7976 a         4562 c         3402 g         9699 t
ORIGIN      
        1 actataccta ttaaaattaa ttaatttgta ctataattat tattgtactt aaaaaattaa
       61 atttagataa aaaaggagat ttgattatat gttatttaaa ggttctgctg ttgctttagt
      121 taccccattt acagaggata ataatgtcaa ctttgaaaaa ttaggtgaat taatcgaata
      181 ccacattgag aatgacacag atgcactagt tgtttgtgga acaacaggtg aggcgactac
      241 tatgagtgaa tctgaaattt ttgctgtaat aaaatataca gttgaaaaag ttaataagag
      301 aatacctgtt atagcaggta ctggttcaaa caatactatg ttgtctgttc atatgagtca
      361 agaagctgaa aaattagggg tggatggtct tttaataata actccttact ataataagac
      421 taatgaaaaa ggtcttaaac ttcactttga aacaatagca aatagtgtta agttgccaat
      481 tattttatac aacgtaccag gaagaactaa agtgaatata aagccatctg ttgtggctga
      541 acttgctaaa atagataata tagttgctgt gaaggaagca agtggagact tagctcaagt
      601 tgcagaaata gctaaactag tacctgaaga ctttgctata tactccggta atgatgatac
      661 tatactacca ctattatcat taggcggaag tggtgttatc tctgttcttg ctaacatatg
      721 tcctaaagaa acacatgatt tagtagctaa attctttgaa ggtgatattg aaggctcaaa
      781 aaaattacaa cttgatatgg atgctttaat tgctgcctta tttatagaag taaatccagt
      841 tccagttaaa actgccatga acattttagg ttttaatgtt ggtgatttaa gacttcctct
      901 agctgaaatg gaagaaacta atcttaatgt gttaaaacac aaattaacaa attttggatt
      961 taaattttag caatatttat ataaataagt tgattttttc ttaaatatgt attattatta
     1021 gaataatcaa attttaatat cctattaacc tagggtagag gtagctgtaa ctaagagtat
     1081 ccaatagtag ctggcaggtg ttaatgattg gaaaaaggat ttacagccga agaaaatatt
     1141 tcttgcaata atattttctg ggattgcaca taacatgtgt aatactgtca cttatttttg
     1201 taggtgatga gctacagggt ttactttgag tgataaatta gacgtacaaa gtataccttt
     1261 gtacgttttt tgtatataat taaaattgtt aatataatat taaagttttt ggatatataa
     1321 aaatattaac atatcttact tgtattacta aaatcaaata aaacaatgtt tttataattt
     1381 aaaattgggg tgaatttata tgaaaaaagt aaatgtagca atagttggtg ctactggaat
     1441 ggtaggtaga acatttttaa aagtattaga agaaagaaat tttccaatag aaaatctatt
     1501 tttattctct tcttctaaat cagctggttc taaagtaatg ttttgtggta aagaatatat
     1561 agtagaagaa ttaaaggaaa cttcatttac tgatagaggc atacaaatag cattattctc
     1621 tgctggtgga agtattagtg aaaagtatgc tccaatagct gcttcgaatg gaatcttagt
     1681 ggtagataac tctagccaat ggagaatgaa tccagatgta cctttagtag ttcctgaagt
     1741 taatccagaa gccataaaaa atcataaggg tataatagct aatcctaact gctctactat
     1801 acaagctatg gtccctctta aagctcttga tgataagtac aaaattaaga gagttgttta
     1861 ctctacttac caagctgtat ctggttctgg tgttaaaggg gttgaagatt tagagaaagg
     1921 aatgaacggt aatcctactg gattctaccc tcatcaaatc tcttacaact gtcttcctca
     1981 tatagattca ttcactgaaa atggatacac taaagaagag atgaaaatgg taaatgaaac
     2041 tatgaaaata cttgataact atgatttaaa aataactgca actactgtaa gagttccagt
     2101 aaaaaatggt catagtgaat ctataaatgt tgagtttaac aatccatttg aattagatga
     2161 tttagtaaaa acacttgaag aagctccagg tgttgtagta gttgataatc ctgctaaaaa
     2221 cgaatatcca actgctgtag aatttgatga tagagatgaa gtatttgttg gaagaataag
     2281 aagagatttt agtgttgata atggtgtaaa cctttgggtt gttgctgaca atataagaaa
     2341 aggtgctgca actaacgcaa ttcaaatagc tgaaatggct ttatcatacg atttagtata
     2401 atatacaaac ttgtataaat atataatttt gtaaaatatt aatagtaact caaaaacaag
     2461 cattagaagt aactgtaata taaaacacaa aaataaaaaa gaggggattg attttatatg
     2521 atatttaaag gttctgctgt tgctctagtt acaccattta caaaggataa caaagttgat
     2581 tttgacaaat taggtgaatt agttgagtac caaatagcta atggtactga tgctatagta
     2641 tcttgtggta ctactggtga agctaatact atgactgatg aagaacaact tgctactatt
     2701 aagtatgttg ttgaaaaagt taataaaaga gttcctgtta tagctggaag tggttctaat
     2761 gatactatgc actcagttaa cttaagtcaa gaagctgaaa aattaggagt agatgctcta
     2821 ttaataataa ctccttatta caataaagct aataaagctg gacttaagcg tcattttgaa
     2881 acaatagcta acagtgttaa acttccaatt atactttaca atgtccctgg aagaacttgt
     2941 gtaaatataa gtcctagcct tatagttgaa cttgctaaaa ttgataacat agttgctgta
     3001 aaagaagcta gtggagacct aggacaggtt gcagaaatag ctagtttagt tccagatgac
     3061 tttgctatct actctggtaa tgatgatact atacttcctt tattatcttt aggaggtcaa
     3121 ggagttatat cagtacttgc taatgtttgc cctcaagaaa ctcacgattt ggtagctaaa
     3181 ttctttgaag gcgatataga aggttctaga aaattacaac ttggtatgga tgctctaatt
     3241 gcagctttat ttatagaagt aaatcctata ccagttaaga ctgcaatgaa tttattggga
     3301 ttcaatgttg gagatttaag attacctcta gcagaaatgg acccagcaaa tttagaagtg
     3361 ttaaagaaag aattagttaa ctttggacta aaagttaatg catagtaaag attaatgcat
     3421 ggtaaaatga taagtttggt caaaagttag cccttaatca taggaggaaa ttatgttaaa
     3481 agttatagca actggttatg atggaaaaat gggtaagatt ttagccgata ctataagaga
     3541 agatagtgaa ttagaattgg tttgtgttgc agctagaggt ctggatagtt atgaagacga
     3601 ccttaaaatc tatgaagata tgagcactat agttgaagaa gcagatgtag ttatagattt
     3661 ttctcatcac tctaatttag ataatatctt atcttacgtt ttaaagacta agacaccttt
     3721 agtaattgct actactggat ataatgatga agaaatgaac aaaatacatg aagcagctaa
     3781 agttattcct gtattccaat catataatat gtctcttggt gtaaatgtat tagtaaaatt
     3841 ggttaaagat gcagctaaat tactagaagg atttgatata gaaataatag aaaaacatca
     3901 caacagaaaa gtagatgccc caagtggcac agctgttatg attgctaacg ctataaaaga
     3961 agttttgcca aacgttgaaa ataattatgg tagatatggc cgtagtgcga aaagacaacc
     4021 aaatgaagtt ggtattcatg ctataagagg tggaaatatc gttggcgaac atgatgcttt
     4081 atttgctgga gacaatgagg ttcttacaat aagccatcaa gctcaatctc gtggtatctt
     4141 cgcaagtggt tctatagctg ctgccaaata tttagttaaa caaaaaccag gattttataa
     4201 tatggacagc atgttgggat aataatataa gtataataat gcacaatata aatcaaacat
     4261 atataataga taaatttact taatactatg tctatataat aaaaactaat taaaatataa
     4321 ataataagaa gtaaatatat actaaaattg acaaacaagt atataataaa agaaatataa
     4381 tgaaatacaa ggagaattaa caaatggtaa atttaaacga tgcttatgaa atagcaaggt
     4441 atataaaaga agttaaaaag aaaacacctg ttaaggtata tgtaaatact aactctttac
     4501 atttccaatc aactgataaa tttaaggtat ttggatgcaa tggttcttat atatttgtag
     4561 gtgactatga agatataatg gaagtattag ataaacatgg gaatgacata atagatactc
     4621 atgtagaata tgatagaaga aattctgcta ttcctctaaa aaatacttta agtgaacatg
     4681 caagaattga accaggtgca ataataagag atatggttac tatagaaaaa aatgctgttg
     4741 taatgatggg agctgttata aatataggag ctgttattgg tgaaggttct atggtagata
     4801 tgaatgctgt tattggagct agaggaactc ttggtaaaaa tgttcacctt ggtgctggtg
     4861 ctgttgttgc tggtgtttta gagccacctt ctgctactcc tgttatagtt gaagatgatg
     4921 ttttaatagg agctaatgca gttattcttg aaggtgttag aatcggtaaa ggtgctgttg
     4981 ttgcagctgg tgctgttgta actactgatg tagaagctgg tgctgttgtt gctggttctc
     5041 ctgctaaagt tataaaaatg aaagacgaaa aaacggctga caagactaaa cttatggaag
     5101 accttagaaa tttagattaa ttaaatgaca atatattaat tgaaaagatt aattaaataa
     5161 taatatacta atttaaaaat taattaaaag gtgtctctat atgaatttaa ttcattagag
     5221 acaccctttt ttataatatt tttatctttt atttattttt ataattctct tattcattct
     5281 catatcattt ttatttcttt ctgataagta tttttatttc cttcaatttt tcaatatatc
     5341 aatgaaacaa actagcgaac tttatataaa ccaataaagt aaactactaa actttatata
     5401 atttaataaa tagaactgct gtttactact ggttgtgcat ctttattgcc acaaagaaca
     5461 actaacagat tacttaccat agctgcttta cgttcttcat ccaaagttac aacattattt
     5521 tcacttaaat tagtcaatgc catctctacc atacttacag ccccttcaac aatcttttta
     5581 cgtgctgcta ttatagcctc tgcttgttga cgttgtaaca ttgctgctgc aatctctgga
     5641 gcataagaaa gatgagtaat tctcacttcg catacttcaa ttcctgcaat atcaaccctt
     5701 gattgtaatt catcttttaa tctatctgct atttcctgac tactaccacg aagagatttt
     5761 tcatctccat cttcagacac atcatatgga taaagtcttg aaacattacg aatagtagaa
     5821 tcgcactgaa tagataaaaa tgtgttataa ttatccacat taaatactgc ctttgttgca
     5881 tctataacct tccaaattac aacaactcct attataattg gatttcccaa taaatcatta
     5941 actttctgtt tttcattatt aagagtcatt gtctttaaag atactttctt acctcttgaa
     6001 ttagttgata tatctacttt ttcgtcagat gaagattttg atacattaat tcttgatgct
     6061 gctgggttta ttgctgaaca aaatggattc acccaatagt acccttcttt ttttatagtt
     6121 ccataataat taccaaacaa aactaaaact atagcctctt taggattaat cattttcagc
     6181 ccaaagaata gaataaaatc tactactata aatataagcc caaaaattaa cataagtgaa
     6241 cctattccag taaatccact atcaagtttt actatcccaa aaaccataat acctattgaa
     6301 actactaaaa ataacacaat cattatcaaa gctgctactc cattaaatac ttttgcttct
     6361 ttctcttgat aagttttttc ttcttttaac atatctatca ctcccatctc agtatattaa
     6421 gttaagatgt ttctaattta ataataacac ttttaacact taaattgttg aatttgaaat
     6481 taatactata atgtatttat atatttttca tacatcttta tacatacatg tgcttagtta
     6541 ttttaatgta ttaatattga tttttaaata aattatacga tatatgaatg tggtatctca
     6601 aaatgaactt ttttgattca tgaggttctc ttttttgtat gaaaccaaat atatttttat
     6661 catttcaaca gtgaaaaaaa gcttatctat actttgacaa agctacttca attttttaat
     6721 gtaagataaa ttgtaaacct attatcacta atattatttt atatagataa tattagtgat
     6781 aaaataaact tatagaaata caacaaattt ttattaattt tataggagga atttatgttg
     6841 aaagttataa ttagcggcta taatggaaca atgggaagag tgttagcaga atgtgtaaaa
     6901 aaagataatg aattaaagtt ggtttgtggt gtagctaggt ctgctgatga aaaaagtcat
     6961 catagtgata caaagcttta caaaacaatg actgatgtaa ctgaaaaagc tgatgtcata
     7021 atagactttt cacatcatga gactttagag ccaatattgt catacgctct aaaaaataat
     7081 acgcctgtag tattagctac tactggatat gatgaaagtg atttaagtaa aatagaggaa
     7141 gcatcaaaaa atattcctat attattatca tcaaatacat ctcttggagt taatatatta
     7201 ataaaattag ttaaggacgc ttcaaagatg ttagaaggtt ttgatattga aatcatagaa
     7261 aagcatcaca atagaaaaga agacgctcct agtgggactg ctactatgat agctaatgca
     7321 attaaagaag tcttgcctga aagcacaaat acacatggga gatatggtag ggattgtaaa
     7381 agaaaagcaa atgaaattgg aatacactcc ataagaggag gcaatataat cagtgaccat
     7441 gatgttatat ttgcaggtga taatgaagtt cttactttaa gccatcattc tcaatctgat
     7501 gaagtatttg caaacggttc tatagttgca gctaaatttt tagttggtaa gcaatctgga
     7561 ctttataaaa tgtcagatat attaggataa attttgtaca taataaatct caatctattt
     7621 ggatattcat attcatcaat ttgaatatct aattgattga gatttctact ctaaaagttt
     7681 ataaatttta tacctacttt ttaatatagt cattctgcta aagattatat tattttagta
     7741 tatagaacta tattgttcat tattttacag tattttcacc atatagactt attaaaaatt
     7801 atattattta ttagtatata aaatcatatt gtttattatt ttatagtcct ttcattatat
     7861 aaactattct attctataaa ctgatacata tccagctgga gaagtgcctg tcgtgcctga
     7921 accaaatgta aaacttaaag taacagctgc tgctctagta tcaactaaaa aacttaaact
     7981 tacacttaca tctgatgaag taagcgtatt tactgctttt gcaataaaat tattttgagc
     8041 tactccattg aaattagtag taattgacat aaatcctgca agtgaaagtg tggcacgtat
     8101 accaaaagat acattatata ttcctccaac tgcaagattt atagtactac ttgttggaaa
     8161 agttatatta gttccattta taaatgatga attaaatgtc actaatgtat tattccctaa
     8221 acttgaagaa gatactgtaa attgtgcatt atttgctgct aacactccag ttgctccagt
     8281 agctcctgtt ggaccagttg ctccgtttgc tcctgttgct cctgtatttc ctgttgctcc
     8341 agtcactcca tttgctccag ttggacctgt tggacctatt ggacctgcta ctccatttgc
     8401 tccggttgct cctactgctc cggttgggcc tgttgctcca tttgctccgg ttgctcctgt
     8461 cgctccggtt gctcctgcta ctccatttgc tcccgttgga cctgttggac ctattggacc
     8521 tgctactccc gttgctccgg ttgctcctac tgctccggtt ggacctgttg ctccatttgc
     8581 ccctgtcgct cctgtcgctc cggttggacc tactgctcca tctgctccag ttgggcctgt
     8641 tgctcctact gttccggttg gacctattgc cccagctact ccggttgctc ccgttgggcc
     8701 tgttggacct accaaaccat ttgctccggt tgctcccgtt gggcctgttg ggcctaccaa
     8761 accatttgct ccagttgctc cggttgctcc cgttgggcct gttggaccta ttactccatc
     8821 tggacctgtt gctcctactg ctcctgttgg acctacctct ccatctgctc cagttggacc
     8881 tgttgctcct attggacctg ttgcccctgt cactccattt gctccggttg ctcctactgc
     8941 tccggttggg cctgttgctc ctgtacttcc tgttggacct gtagaacctg ttactccatt
     9001 tgctcccgtt gctcctgtat ttcctgttgg acctgttatt ccatttgctc cagttgcccc
     9061 tgttgctccg gttagtccag ttggacctgt tgctccggtt gctcccgtat ttcctgttgg
     9121 acctgttatt ccatttgccc cggttgctcc tgtatttcct gttggacctg tagaacctgt
     9181 tattccattt gctcccgttg ctcctttgtt tcctgttgga cctgttattc catttgctcc
     9241 cgttgctccc gtatttcctg ttggacctgt tattccattt gctcccgttg ctcccgttgc
     9301 tccggttgga cctgtagaac ctgttgctcc atttggtcca gtcgctccca tatttcctgt
     9361 tggacctgtt attccattcg ctcctgttgc tcctgtgttt cctgttggac ctgttattcc
     9421 atttgctccc gttgctccgg ttgcacctct aggtccagtc ggacccgttg gaccagtagc
     9481 acctgttgga cctgttggac ctgttggacc tacatcttga tataaatttg gacctgaaat
     9541 atcactcata taattatcct ccctttttaa agttagagta atactctata aatattgagt
     9601 attactctat ataattatat gaatcgacaa tttcttatgt gataatgtat ctatttactt
     9661 atatcaacat caattctttt attcttagat gtcatcacat ttttataaaa tattttacaa
     9721 ttataaatac aattaaaaaa ttcatttccc accaatcatt tgaaattttt ttattatgtt
     9781 ataaaaaaga tatagttgaa ctgcacaaga ttaatttaat gctactttaa ttgcttttta
     9841 aataaaaaaa cctcaacacg attaataaaa atcatattaa ggcatccaca aaatcatata
     9901 attgcttatc tatcttcatc agtgacattg aatatataag gaacatttct ataataatcg
     9961 ccaaaatcaa acccatatcc aaccacaaac ttatcttcta tttcaaatcc acaataatct
    10021 ggcactaaat cgacttttct tctacttggt ttatccaaaa gaacacagca ttttaaacta
    10081 gcaggatttt tagcttttag atgttttaac acaaaatcca tagtaagtgc agaatcagta
    10141 atatcatcta caactaaaac atcatatcct gctatatcag tagtaacatc tgaaactact
    10201 ttaacctttc cagtactttc ttcattattt ccataactag atgtagtcat aaaattgact
    10261 ctaagtggta aatttatatt tctaactaag tctgcacaaa atataaaact accttttaat
    10321 agagaaacta ccataagatt tttatcttta taatcttttt ctataattgc tcctaattct
    10381 tttaatttat ctttaatttt ttcttctgaa tacaacactt cccatttttt tgtttctata
    10441 tccataaaaa gacctccaat tgtaataatc cttcttaatt ttataagaga ttgctcactt
    10501 tttcaatact cttattcaat attagccaat attattgagt ttttatgttc attcaacaat
    10561 tattaacaag gtatcttata ttttaagtag ttcatatatc catactaaat tacatactat
    10621 atattttaga caaaaaaaga aaaaacttgc actaacaagt tttttctatt atacataaat
    10681 ttatacataa tcatatttta ttagagggta ctaattaaat ttatttatgc tgtttttgtt
    10741 attcttcata atacaaaacc atattataaa gtactagtca actttattca tatcattcat
    10801 tacatttaag attatttcat ctgttataga catacctttg tcacttactc tacctaagtt
    10861 ttgtatactt tgctcaacac ttgaaccaac tataccattt agtggtggta caaaacaatc
    10921 ttgcttggct attatagcac tttgtatagc tgcagatgca gcagaagata attttagtgc
    10981 acaaccagct ttcgcaccat cacatatcat tccacttaag tcagctatca tattttctat
    11041 agcaccttct atttgctttt cagtaccatt cataagccaa gagataccag cagttgctcc
    11101 agtagatgca gcaacaccac atccacatac agcagataat cttcctgtat aattcttaat
    11161 ataaccagtt actaaatgag atatagctaa agcttttgct agtctttcat cagattgtgg
    11221 gaatttttta ttgtaagcaa cgattggaag aattgctgtt aatccgtggt ttccacttcc
    11281 atttgaactc ataacaggca tttttacccc tgccattctt gcatctgaag ctccagcagt
    11341 taacatcatt gcataattta taacatcatt gcctaaaaga ccttcttcta tagacttttt
    11401 aattccatat ccgacaccaa taccagtttt ttgttttaat ccatattcag ccatttcttc
    11461 attcattttt actccatcta gtaaaaattc tatatcttta aaatcaatgc cttctacatt
    11521 ttttattaat tcttgtatag ttactgtatc cattaaactt tcaacttttt ctgctgaatc
    11581 atttgaagct gatacttttg gttcattgtc taataatact tctccatctt tttctaagaa
    11641 cgtaaaatta tcatgtttag ttcttattct aactttagca atatgatttt ttccttttaa
    11701 tacaacttct ataaacactt tctctcttgt atcagcaggt gttattgata gaggagtatt
    11761 atccatataa tcctcagcta ttttaacttc ctcttttgtt agagtctcta acacacttaa
    11821 tccgttttca gaatgtccac ctactatacc tagagctgct gcgattttta accctaatct
    11881 ttcagtacct ggtataccta cgcacatacc atttttatag atactagggc taactaacat
    11941 tcggttttca actatttctt ctcctaataa ttcctttgcc tttgcacatg ctagagctaa
    12001 agctactggt tcagtacatc ctactgctgg cttaacttct gcctttaaca tttccactaa
    12061 ttcttttctt atatctctca ttatatataa acctcccaat taagtttaga tttagtactc
    12121 aaataattaa ttaaataatt tcttatctat atgtatagca aaatacatgc catatttttt
    12181 atatctgttt atatttttaa ttttatattc tatagccatt ggtatgacta cttctattta
    12241 tgcatttttt ttatttgcat aggatattta tattctttaa aatggagaaa catatgacaa
    12301 tttcgcaaaa agacaaaaaa aagtctcaaa atgagattct atatcatttt gagacgactc
    12361 aaacatatta ttttttaact tataaaaact attttttaat tcataaaaaa gattatgcaa
    12421 aacacttaca atagaaattt aaatccttct ttaaagttat acatttagaa aaatttaaat
    12481 tttatacagt tttttattat attactatga aattatatta tatttttcaa tttttctata
    12541 taatgtagct cttgatattc caagtgcttt agctgttaat tctttatctt ttttatagtc
    12601 tttgtattta tttaaagctt ttattatctc aattttctct agttcatcaa gtggaattat
    12661 cctatcgctt ctaacttcca actcttctac agaggtattc ttaatttttt gaggaattac
    12721 attcattgtg attattgaag aggaggacat attcacacta tattcaataa tattctgcaa
    12781 ctctcttata ttccctggcc acttatattc catcatagca tccacaactc tgctatctat
    12841 gtcaataaca tttttatcta atttttttga atactcttta accatatgtt tgattataag
    12901 tgggatatct tcttttctct cttttaaact tggtaatttt ataggtataa catttaatct
    12961 ataatataag tcttctctaa aagttccatt ttgaaccatt ttttctaaat ctttatttgt
    13021 tgcagcgata attcttacat ctattgaaac atttgattta cttccaattt tattgagttc
    13081 cttttcttgt aatactctca ataactttga ctgtaaatgc aaactcatat ctcctatttc
    13141 atctaaaaat atacttcctt tatgagctac ttcaaacatt ccaagctttc cacctttttt
    13201 agctccagta aaagctcctt cttcatatcc aaataattca ctctctaata aattgtctgg
    13261 tatagcagca caatttacag ctataaaagg attatctacc ctattgctat ggttgtgtat
    13321 ggctcttgca aatagctcct tacctgttcc actttcacca gtaataagca ctgttgatgt
    13381 tgatttagaa gctattaaag cctctttttt agaatttctt atagctacac tttccccaat
    13441 tatattttcc aattttatcc tataatcttt attcatctta ttgtagtttt ttatagcatc
    13501 tcttttatct ataaaatcaa taacatatcc ctttaactca ttttttaaga ctattttatt
    13561 gatgttatat attccctttg aatcatatcc gtatctttta taaaaaaaac tacaggattt
    13621 atccttctta aagttattta tagaaggttt ctttataaaa tctaattcga caaatatgtt
    13681 tttaccagtt atattatctt ttatattgaa aagctcttta aatttcgaat tatatttatc
    13741 aatctttccg tctacatcaa tagacacaac agctttgtcc atattgttta acagtgtctc
    13801 taattttttc ttttctaatt caagctcata cgttttaatt tgagctttta atttattaga
    13861 tattaagtct gccattttgc ctaaaaagtt tataagacca cttttattat tttctataat
    13921 ctgtgcttgt tcctttgtaa aggctataag gcctataaca ccatgagaga ctccatcaca
    13981 tataatggga caacacacct ctgcaaactc tacacaagcc ccacttttat agcattccct
    14041 acatatatca ctactctttg ggtcatcaat tagtattgta atctgctctg taagtgattt
    14101 tttaaaagcc gtatatttat ctactttctc acctattttg tctatatata ctcctgtacc
    14161 tgcaatccta aataagtcct tatttactat agttacatct atattgagta ctgatactat
    14221 agcttcagct atattttgaa tatcagattt tatatattct aaacctatca taatcatcat
    14281 tccttatttt tctttaacat aagtatttgt cttatttctt taagaatcaa gtatatctta
    14341 tttaaagtaa attggattga cattaggatt acaaagagca taacaattat tattaactca
    14401 taatttattt ccatctaaac atctccttat aacaaaagac taggcttaac ctagtctttt
    14461 atattatatc catatttata ttttaactca tttatcttct ttttcccaca taaatatatt
    14521 ttaatttatt tgactcttta atcataccat caaagtttaa tcttgaaaat atattgaaca
    14581 cttcatgtgg atagtgaatc tttgcaacag aaccacaaaa tttatgttta agataattta
    14641 acttaacttt taaattcttt aagtttaaat cctctaatat aacctctcca ttgcccttta
    14701 gtactctttg tatttcagat gaagcttttt cacaatctgt aataacatta aattggtcaa
    14761 ctattattat cttgttgaac tgattatcat caaacggtag aaactctaac ttacctttaa
    14821 ctacattgca attttcatta tacactagag aataacatac atcttcatag ttactattct
    14881 ccagtatagt tacagaagtt ccaagacctc tcaatctctt gcctaggttt cctatatgac
    14941 caacaagcaa tattttatca cctacattat actcaattgt atccaaaaaa cattctaatt
    15001 catcagatgt atatttagct ttaactggct gtttatttat ctgacataat atgcttttca
    15061 caatgcttca cctcttcatt tcctcaatta taaattatgt acagattaca ttataataaa
    15121 tggcgagcaa ctattcaaga gatatttcat tagtattttt ttaaacaatc tttatatatt
    15181 ttaatatgat attataccaa acttattagg attttattca taaaagtcag tctacttttt
    15241 atgtttattc attgttattt tcatccaatt tttttatttt cgatatagat acttcatatg
    15301 ccattttttt aaccacatta ttttcatcta tttttttctc atattctcta ctttgaaccc
    15361 taccccaaag ctgtatccta tcaccaacct ttagattctt tgcaaactta gcatttcgtc
    15421 cccaaactat tgatggtata taatcagatt tattataagg tctgtttata gcaactaata
    15481 aatcggttat ttcccttcct aaaggtgtct tcctatatac aggttcctta catacatatc
    15541 catctaaaaa tatactatta gggtctttat tttcctcgtc tatgcttttt atttctctga
    15601 caaacactgt aagcactaat ctatttttat tatttaatgt tttattatat gatcttaatt
    15661 gacctacgac attgactaaa ttttgtttat caaggtcaat atcttggaga agtctttctg
    15721 atacagtcat tggaagtata tcaaaagaat cgcttaatct gtttattttt attttcatat
    15781 tataaaattt ttctccaaat atctcatggc taaattctag cttattgtct aactcccctc
    15841 ttaagtttac tacgttattc tcatctttaa cagctatcat attttatccc tccacaattg
    15901 ttaattctta tatttctggt atcagcttat ataataaata tatttataag ggatgtattt
    15961 tattacaaat ctttgattgc tgtttaaaag tttttatttg gttataaatt tcatttaata
    16021 tttcactttt ataattataa aattactatc tttttgtacc atatatacta attaaactat
    16081 ctatttcagt tttgtattaa ttataaaatt actgttatac aattaataag tcatatataa
    16141 cttaaattta tttgctctat ataagcaatt tcaaggaatt aaacttattt aaataattca
    16201 gataagtatt aagtcattat aaacttattt ttcttaacta taagttatat tagacttatt
    16261 acggcttata tttttattaa tctgtattaa tatatgaaca tttattaaac ttattgatta
    16321 taaaatttaa atatttagct ttaaattatg ctttattatt taaaaataca acgtttttta
    16381 cattaaatct actaaattac tatatatatt acataattgg catgttaatt gctttatata
    16441 ttagtaaata aaaaaactgc tctaaaatat taattcaata ttttaaagca gtttataatc
    16501 tttattatta gctgtaatat ttgataaaca ctaagtagtc tttaactaag cagtcttttt
    16561 gtttttattt gcagccatta tcattgtagc agaagtataa gctataacta ctattactaa
    16621 tgcttttaat atatccattg gtagagtttt tactatatat gcagctacga aaactcctat
    16681 taaaccacat atagctatta taactgatgc ttttttagta tatgcgtctt cttttatgaa
    16741 tttcatactt gcaactggca ttaataatgc acaagaaccc ataactattg ggaatgcaac
    16801 ttttgcagac attcctaaga aataaaccat tgccatacct ggagcatata gacctatacc
    16861 agctgtcatt aaagctccta gtataaagtt acctattaca cctattacta atttaacacc
    16921 agtaagacct aattcatttc ctccacctgg taatacgtta acccaaggat gacctaataa
    16981 cattaatatt gctgttaatg ctaaagttac acccataaca agttgtattt ttctctcgtc
    17041 catcttagct attattcctg cacctatgta tgaaccaact gctgctgcca caaccataga
    17101 tactaaagta acaccatcaa ctggtatgat tgatgtaaat atgaatgctt ctgtaacaac
    17161 tggaatagta tgtgctacgt ttaatgtacc aggcattttt ttatctggta tatttagctt
    17221 taaagctttt gacattgcta ttgttggagc aaatgaccct attcctaatg tatcaaagaa
    17281 gtctgttata aatcctgtta cagcaacttt tggagtactt tgttcttcta gtttcccctc
    17341 tttgtttgct ttagctacgt ctttaccaaa cacaactgca aagattgctg cgaatactac
    17401 gtataatgcc tttaagattg ctaccataat ccatcctcct atttttttat tttttcatat
    17461 attctgaatc ttatagccta aaagctatag aatagtagcc gataagtggc tactattcta
    17521 ttttaatttc ctaatttatt tattacttat ttaagaataa atccatgttt aagtggatct
    17581 tcttcatcta ttacaaaatg attaaatcca gttatataag cagaaccagt tattttaggt
    17641 actacagcat tgaaatctgc aactttagtt tcttcaacta tttcaccttt gaataaagtt
    17701 cctaatatac tttcatatac gaatttttct cctactttta attcaccttt agcatgtaaa
    17761 gtagctaatt tagcacttgt tccagttcca catggagatc tgtcaacttg accttgacca
    17821 aatataacta cgtttttgta agtagcttct ggatgagttg gctcatcata tatttcaact
    17881 aagtctacag tttttatatg agctaaagtt ggatgttgta tttctatttt ctcatttatt
    17941 atatctctaa gtttcatagc tagttcagtt aatttaccag cattttgagg ttcgattttt
    18001 aaacctaatt gacttgcatg tattatagca aagaagcttc caccaaatga tatgtcaaat
    18061 ttaacagttc caacacctgg taagtcaact tcaacacctt ctttatataa gaaagctggt
    18121 acgtttaaga atgatacttc tttagctttt ccatctacaa ctgtaacgtc tcctcttatt
    18181 attcctgctg gagcttccat aactacatga gttacaggct ctactgctgg aactacacct
    18241 gtttctatag ctgctgtcat agctcctatt gtaccatggc cacacatgtt aaggtatccg
    18301 ccaccgtcca tgaagattat tccaaagtca gcatctgggc aacaaggttg agtcattaca
    18361 gaaccaaaca tatcattatg tcctcttggc tctaacatta tagcagttct taaataatct
    18421 agattttctt ctaaatattc ttttttctca ggcatagaat ttccttttat gttaggtatt
    18481 ccacctacaa ctattctagt tgcttcacct gctgtatgag agtctatagc ttgtatactt
    18541 ctgctaaatt tcatatcaaa ttccccctta ggtttatttt gcgtctttac gcatttttaa
    18601 ttttaggaaa gccaatgctt ttttaacatc aacttcgtcc atatgattgt ctccaactac
    18661 ttctgtttct atgtagcctg ttgtcttttt ataatcaatt atggtagctt tatctaaata
    18721 tggattggta attactaatc catctactcc cataatagta agacctactg taggtatatc
    18781 tgattttaca aactctgcta tagcatttgt aaaatcaata tgctggtttc ctgccatatc
    18841 taaagttata atcactccat caggtcgcaa tgattcaatc catcttccaa gtctagttgc
    18901 taaaaatgtt ttccattcat tgtgctctga tgtcccaact acaaccactc ctaacaagtc
    18961 tatgtcagaa tcattatcta tcaaatctag taatgggtct ctaaatatat gaagtgatgt
    19021 gtttttcatt gatgtagaaa ttcccacaaa actcattcct tacttaaatt ttttacccat
    19081 gttttctcat tttaagtttt aaaaatgcca aagctttttt agcatctatt tcatttgtgt
    19141 tgttttctcc aacaacttct gtctcaatac cttcttttga tttgttcata tcaactatgg
    19201 tatccatata ttgattgctt acaacaaact tagcttgggt accattaaat gtaactccaa
    19261 ctacaggtat atctcttttt cctatttctt caaatgtgtt agcgtagtca acatgtgagt
    19321 ttccccaacc atctgatgaa actatagctc catccactct catggcttcc agccaagcgg
    19381 ccgttctttg tcccacaaag gttttatttt catttccatc tggagttcct actactatta
    19441 ctccaagtaa atctacatcc ttatcacttt caactatgtc taaaagtggg tctctaaagt
    19501 gatgaagaga tgtttctttt gttgaaggtc ctattcccat aatatccacc tctcttaagt
    19561 catagctcta agggcaccat ctctgtattc attaggagat ataatcatag gtacatttcc
    19621 catatctata attgatgttc ctccttcaaa tccactaggt tcctttgaga ataactgatt
    19681 gtcatacata gcaccttgac ctgctatttg ttttactatg acaacctttt ttgcatttgg
    19741 tcgaattttg tcaaaatatt cacgaacttg agtagcattt cttccgtctt ccattttcaa
    19801 agacgctctt atttcttgta taaaatcatc acatgctctg aatgctgcaa gtggaagtgt
    19861 tctttcaaaa ggtaaaccac cctttaaagt tacatctaca tggattatat aatcttcaac
    19921 tgatggagtt ccatatctcc caaataccat ttgttctttt aaaattcctt ctgaagaacc
    19981 aaattcatgc atttgatttc catcttcatc tgcaccagtt aacattacat atacaccagt
    20041 taaagtatgt gtaattccct ctcccagtct gcctaaaact tttgttgata ttggtataat
    20101 gtccataata gtattaattt ctctgtcata atctccaggt tttataatgt ctacttttat
    20161 gtctgttatt aactcttctc tttcttttat ttcatctata cttgataaat ctattgtaag
    20221 tacatctttc tttatagaag tttttgaacc gaaattaact tcattcatat gaaacggttt
    20281 tataacaagt cttcttatta ttttttcttc cataatatcc acctttgttc ttaaaacccc
    20341 tctcctaact gtgtaggaga agggttctat attttcaaat ataaattaca ttttaaacgt
    20401 gagctttata ttcgtatggt aatggaacta tcttaccagc agatggtatt gcaactaatt
    20461 gctctaatgt atctcttaat atatgtgttt gcatttctat gttatgtggt tcaccagcat
    20521 tagcacccat tggaactaat ggagcaactg ctctaggagt tccgttttgt ctaactactg
    20581 gaggaagagc tgcgattatt attgtaggta ttccagcttc ctctattgct ctctgcacga
    20641 tcacggcagt tctatggcaa gtacctcagc cagctgtcat aactacaccg tctactccct
    20701 cttccttaag tttagcagcg atagcaggac cagtttcttc agtgaagaca tgttggtttc
    20761 ctccaccacc catgaatgca tagtgcatag gagctactcc tttgataaat ccttctgcag
    20821 ctaattcatg taatctatct atagggaaca tacagtttat atctttattt acatctccat
    20881 tgtcatatcc accatgtgat accatcattt catcaactgt agctgtatta ggtatagctc
    20941 taaatgtagt atctcctgct agattaaatc tcttgtcaga ttttaaatga acaccagctg
    21001 ctgtagctaa agctatagtc atatctttta attctttagt tacaggagtc caaactggag
    21061 gaggtgttat tggaacgaat atttcagatt gaagtccttg tactgttgta aggctcatat
    21121 atttcctctc ccttctattt tcaaacgatt atttattatt tttcttcttg ttttgattgc
    21181 atttctgctt gtttttcttt ttctctccta atattttcta catactcttg attaggctct
    21241 ggacttaata cctcaggata gcttagcatg aatccaactt cttgacctat cttaagttcg
    21301 tagtcagtta taagcattct attaggtatt ttaaattgtc caagtctacc ttttaggtct
    21361 atagtcactc caccaccttt gatgtcaact ataatacctt cataaaaagt ctctgatgag
    21421 atatatttta acctatccat tataattcta cctctctata ctgttttaaa cttggatcta
    21481 aatttatgtt attgtctaaa tcttagttaa tatctaaatt ttagttttcg tctttttcgt
    21541 atatttctct tctcttctta ctcattggta aagattgctc attttcttct aatactattt
    21601 tttctcctgt aactttttct atagcttcta tgttatttaa tttaacattt ggattccatt
    21661 ttctttcagg agctttaact tctacacctt ctatagcatt tttaagcata gccattattc
    21721 taacagcatc ttctggagct aaagtattgt tagataatat ttcattctct ataccttgct
    21781 tagacttatt gttgtctacc atgtgagtca tgtatttatt accaacaact agagcacctt
    21841 gaacagctga gaaacttaca ccaactactg gtatacctct catacctatt tgctctatgt
    21901 gagatgcgaa atctatatgg ttgtttccaa aaccttcagt agttactaca gctccatcaa
    21961 cttccatagc ttcaactaac atacctaatc tcttagatac atagaatttc tcagcattta
    22021 cttgtggaga accaacaaat acaacaccta ctaaatctat ttcttcatct tccatagctc
    22081 ttattactaa tggctctctc cagtaatgtc ttgacatttc ttttgatgca ggtcctatac
    22141 aagttaatgc atggatacca ccatctaata cttcaagtgg agataataca actggcatat
    22201 ttcctaagtc aacgtttggt tgagctccta atgtaccaac tggctcaact ggtaatatta
    22261 ggttatcatg cattgcacct tgtcccatta tttcttttat aacaacaact ttctttttgc
    22321 cttctcttct gtattgctct attacttctt catcaactac taaagagttg tcaactttct
    22381 ttaatgcttc tcttatttct tgagttacat agtcaaatgc acggtgagca gctaaaggtc
    22441 ctggtctttc catgttagtt cctgctttta ttgttacttg acctttgatg aatatttcac
    22501 ctttgtcagc agcacctggt ctaccccaca ttatagtagt atttaactca ccttctgaag
    22561 aaccaaattc acctatttga actccatctt catctgtacc agttaatacc attacagctc
    22621 catctataac tctagttata cctgaaccta attcgccttc ttctttagta gcgattggtt
    22681 gtatatctaa tacagcttca ctgtaagttt catatttatc aggagttatt atttctaact
    22741 tcatatctac aactaactct tgagtttcat tagcttcttt acatatttct tctgcatttc
    22801 tgatgtataa agtagttcct tctattttag tttcatcagc aaattcaact ttatctattt
    22861 taaagtgttt tttagttaag cttcttaaaa gtttcttttc ttcaactttt ggagctactt
    22921 ctgctgctgc acttgccact ggagcagcaa cgtttgctac tggagcagca actcctgctt
    22981 gaccagctac tgataaaggt atctctaagc ttatgttctt accttcacct atgtgtattt
    23041 taactgtttc acctgcaact cctgtgctta ctggaactac agctgcttct gatactggag
    23101 ctgcttcttc tactgtttct tctactactt cttctttagc ctctcctgct ttgtatcctt
    23161 ctacgttatc tgtagtcatt ggagaaagag catctaaagt ttcttttaac ttagctccta
    23221 gtacttgacc tatagttaat ccattttctg gtattgttaa taatccagaa tcctctaagt
    23281 ctgcaaatat tgctggatct tctaagtttt ctggcgctat aattgttccc gcttcaaatc
    23341 tacaacaaca aactgctggg tcatttgcat gggcttgagc tgtttctaaa gttattgaca
    23401 tagtataaaa tacctcctta cattattttc ttatttatta ttttaaatct caattcctca
    23461 acaggtaacc ccttaactga gaaattaagt aaattaaaac ctacataatt tgttatgatt
    23521 aatattattt ttttctcact taaagtcact ttatattata agcaattcct atgccaactt
    23581 attaacaaaa agattttttc tataatagat tcttcatcta tcgctatttt tagccaaata
    23641 ttatataaat atttatttta aatatgttat tttttgtatt tgtaaacatt ttctcaaata
    23701 aaaaaagtgt caattaatca acactttttc atttttttta acacatctat atatacgaca
    23761 ctcatacaag cattggtctc actaggtttt aaattaaagt ttatcatttt gtcaaaattt
    23821 agtcatgtgt catattttag acacatttat tttgtattgg tctatcttat aatacaatgt
    23881 tgttctgggt atatttaata tcttagctgc tttagcttta tttccattag acattttaag
    23941 tgccttggtt atattcttta tttcaatttt ttgagtggcc ttcgttaaat ctaagggata
    24001 ctcttcttct tcagtactat tatttgtaga atccatcata taacttggta catcatccac
    24061 atctatttta gaattttgtg acaatacaac aatattttca atcgtatttt ttaattctct
    24121 gatattgcct ttccatctat aattctgaaa tatatctatt acctctttgc taattgtaag
    24181 tacaggctta ttattttgtt tgcatatctc ctccaaaaaa ctatgaacca ataatcctat
    24241 gtcttctttt ctctctctaa gtggaggaat ctttatttca acaacattta atctatagta
    24301 taaatcttct ctaaattttt ctgcctttac cattttactc aaatctttat tggtagctga
    24361 tattattcta ggatttattt ttatcgtagt atctccacca actcttctta tttctttttc
    24421 ctgtaacact ctaagcaact ttgcctgcat actaagaggt aaatcggcaa tttcatctaa
    24481 aaatacagtt ccatcctttg caagttcaaa tatgcctatt cgaccttttt tacttgctcc
    24541 tgtaaatgca cctgattcat acccaaaaaa ctcactttca aataactcat ttggtatagc
    24601 actacagtta acaggtataa attgaccttt tctctcacta taatcatgaa tagctcttgc
    24661 aaatacttct ttacctgtac cactttcacc ccaaataaat atactactat ttgttctagc
    24721 cacttgtttt gcaatcgctt tcgatttttc cagtttataa cttttaccta aaatcttatc
    24781 aaaactacct ttagataaat ttttgacttc gttttttaga tatttcaatg tatcatttac
    24841 tttttcaagt tcaagggcta atatcttagc ttctgtaaca tcaacttctg tacatacaac
    24901 accgataaat tcttctttat aaaatatagg atttgcatgg actaatgcat acatattttc
    24961 ttttttatcc gtaaaataca ggtcactcat tccaacttta ctatttagca ctctttctga
    25021 aatagtattt tccaaaaact cattcatcgg tcgaccaact atgtcttcag actttatatc
    25081 atacctttct tccataaact tattccacaa gataacaaca ccttcattat ttatagcaca
    25141 aattccctct ttaatttgac ctataatata cttcaaagtc aatccataat cttctaaatg
    25201 catatataaa tagtctctaa tatgctcttg ttttagaact ccaataattt tattatctct
    25261 aagaacagga agaatcccta tattttttaa tatcattata tctctacatt catctaaggt
    25321 taaaccttca ttgacatata ttacatcctt cttcattata agttttactg gttggccttc
    25381 atacttttta tgcatctcat aaagattgtg tatatccgtc atggatataa tgccttttaa
    25441 ttgaccagat gaatctatta ccattagagt ttttgtatta ctttttatca tttcttttat
    25501 tgcatcttca attcttgtgt cttcgtctat agttgtaaat tttgtatcca ttacctcttc
    25561 tacttttttt acttctggta tcgaaaacat aaagtccccc ctttataata gtacatcatc
    25621 aaaacttatt tatatatat
//
LOCUS       DAMJVM010000048        25439 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.049, whole genome shotgun sequence.
ACCESSION   DAMJVM010000048 DAMJVM010000000
VERSION     DAMJVM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25439
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.049"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(121..423)
                     /locus_tag="PC523_002116"
                     /pseudo
     CDS             complement(121..423)
                     /locus_tag="PC523_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895500.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase CAT"
     gene            complement(416..994)
                     /locus_tag="PC523_002117"
     CDS             complement(416..994)
                     /locus_tag="PC523_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2475638.1"
                     /translation="MPTKGEQTKQFIIDTSIMLFSEKGYSNVTMKDICEACNLSRGGL
                     YRHFNSPKEIFISILNTHKHYSQNLLDKGIKENISAKHMLEYFFQQQKADINGKYCGF
                     DFAIHEFAFIEKDQRDYMNKRYSSAITMLSNLIDYGQSKSEFKKCDSKSVAIHIMFLF
                     DSMKTSASILKLTNKDIDNQIKIIKDMVILYE"
     gene            1355..3451
                     /locus_tag="PC523_002118"
     CDS             1355..3451
                     /locus_tag="PC523_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HDF2475639.1"
                     /translation="MIKVVYMRKNKKARKMIIFGFLMCLLIVIIVFLAKFILDGLNKN
                     KPDEVFKQYMSFANKKQYEKMYDLLDEKSKSENKKEDFVLRNKKIYEGIDAHNISIKI
                     NDIKKNKNNDKLINYDSKMDTLAGEILFSNEVLLTRDRQKNYKIKWQSSVIFPELEED
                     NTVRVSKLKGKRGRILDRNGVMIAGQGLASMIGLVPGKMSDNIEDLKKLSTLLNVSVE
                     QIEKKLNASWVKENSMVPIKTIEKIKENPDGTVKEEDKELQESLLSIPGVKISNTEVR
                     VYPFGEKTGHLTGYVQNVNAEILKEKEGKRYNANSIIGKIGLESLLEDRIRGIDGYEI
                     IIADRYGDKKETLVTEPKVDGEDVKLTMDSKLQSKLYDQMKNDKGCAVVMNPKTGEVL
                     SLVSSPSYNPNEFILGMSEDRWNELNKNENKPMYNRFKARLCPGSSFKPVTAGIGITT
                     GKINPNENFGHSGLSWQKDSSWGSYKVTTLKDYGNTVNMENALIYSDNIYFAKAALKI
                     GEDVLSKELLKLGFDESMPFEFGLSSSKFGTDNKFETEIQLADTGYGQGKLLVNPVHM
                     ASIYSAFVNDGDMIKPHLEFKKNLQAEFWKKGVFSKEAVKIIRDDLLQVVESPNGTGH
                     SAMIKGIRIAGKTGTAEIKASKDDVTGTELGWFNAFTVDGNNDKQYLAIAMIEDVKSR
                     GGSHYVMPIVKSVFED"
     gene            complement(3804..5603)
                     /locus_tag="PC523_002119"
     CDS             complement(3804..5603)
                     /locus_tag="PC523_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HDF2475640.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSNTNRTSMIKNSPNSIINNSNALNDF
                     SVSLNQVNFDILNILLLILWIIGILIMIVITIHCNIKIKSMKKSIQILRDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNRDMLINYIMCFFQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDNHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNKQTMYEDLSKYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRDNSHIPWDGKHHSNISWNEDQDLFSAMENS
                     VNWYFQTLDKKTGIKNIQTYLEQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFNFEDKNIETVKDSLLISKKDGVSLSGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGISASKITLSILNDKKIYNP"
     gene            complement(5606..5992)
                     /locus_tag="PC523_002120"
     CDS             complement(5606..5992)
                     /locus_tag="PC523_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2475641.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            complement(6363..6989)
                     /locus_tag="PC523_002121"
     CDS             complement(6363..6989)
                     /locus_tag="PC523_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HDF2475642.1"
                     /translation="MKINIKSTLNEFIKQNNLFDDNRIISYLPNITIDTDKIKTIMIN
                     EVVPTNTNDDFYSIDKDADYLKTTIPLFNNAGIEVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(7132..7389)
                     /locus_tag="PC523_002122"
     CDS             complement(7132..7389)
                     /locus_tag="PC523_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475643.1"
                     /translation="MILNRTELFRGYSLDRFNEICNILSVNKIKYKYKVKNNTYSKKP
                     FYNEITLGNLGQKEDFSYEYLIYVHKDDYEQAEALINSKLR"
     gene            8167..8871
                     /locus_tag="PC523_002123"
     CDS             8167..8871
                     /locus_tag="PC523_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2475644.1"
                     /translation="MKDLIIETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTDGRIIFDRKPWKRESLEKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLNDPELLILDEPTNGL
                     DPFGIQELRELIASFPEKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            8868..9638
                     /locus_tag="PC523_002124"
     CDS             8868..9638
                     /locus_tag="PC523_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF2475645.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QINSWAWESLLALIFNWWSMMFLSIGFALFATLVASQEKKAGNYRALRTHNVSPMTIW
                     INKIIAMAVYSFISTLVLIVVTIMTGLILKAGPIPFGQIIGASIVCWVGSLAILPIQL
                     WLATWKGMFLSMGVGALGMIFGVLAASKPFWVAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            9635..10414
                     /locus_tag="PC523_002125"
     CDS             9635..10414
                     /locus_tag="PC523_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF2475646.1"
                     /translation="MIKLLSSEWLRAKRTAVQGLTLYMPIIFSLCVVAYLTIKSGSTQ
                     EFAFEGFFTIWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMNIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGAKVWAFVPWTWPVKLGMLPGTYFIKEAGTISTEV
                     FYSKMMQSATVVFIVVAIGLIIFLIGGVIWFKIWEGRKSYE"
     gene            10466..11158
                     /locus_tag="PC523_002126"
     CDS             10466..11158
                     /locus_tag="PC523_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2475647.1"
                     /translation="MSKILIIDDEIDLVMLLEDELKAKGHEVLIAYDGQAGIELSKNE
                     PDLIILDIMMPKMDGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFEKLCLYMKERAIKIDGKDIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            11177..12601
                     /locus_tag="PC523_002127"
     CDS             11177..12601
                     /locus_tag="PC523_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2475648.1"
                     /translation="MKNQSLATQFRRTFIFIIIASIVATVITYVFAFYLFIYSLNKDI
                     YPPNYYERQVPRIKKYIDEKNIALLSQSNEEGLKRTIRGDGMLYQVVDDNGNILYGNN
                     PKKPFKTKEELFNNFINTTVRKGGYIYTVPIKGTNGKIEGAVILFYQVKMTFANSMGR
                     LTFAVIVIAMFSPFLYVIGFTIWLSKQFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKNSLSAQWKMEQERVEMVEALAHDLKSPLSIVLGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNPVPINLPEFLVQ
                     KVQDYELQAHQKDVELILKMYGNIQSPIQIDLDRLTRIFDNIISNSLQYTPSGGNISI
                     IAKAEKNCISYEICDSGSGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(12842..15310)
                     /locus_tag="PC523_002128"
     CDS             complement(12842..15310)
                     /locus_tag="PC523_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2475649.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITLGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLIGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGASENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRGIGAEPNQIYKIVILQILILCTIAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTSISLI
                     FSFISALPSAIYASHVSPKIAMAGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSILDKIDNM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSTLEDFK
                     NGSICFIKNPLAISTPGVKTKYTNLKPKDNITINKKQLDIYSTVDKMFFLQGNGWVNG
                     VDIIVYDSVYNTLTNKNKFNQINIYAKDKSDLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIELLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNSSLY
                     KMFLWEGVYYGIFAAILGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISILAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(15313..15981)
                     /locus_tag="PC523_002129"
     CDS             complement(15313..15981)
                     /locus_tag="PC523_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2475650.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSIIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHSEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKDYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            complement(16155..17315)
                     /locus_tag="PC523_002130"
     CDS             complement(16155..17315)
                     /locus_tag="PC523_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2475651.1"
                     /translation="MDKINNMSLKKVFFVLTLSSLIIASILTTFSYILLNNLYNYIQD
                     KYLKTPINTGFITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIKNLDENISIIAKQEGKSFTLKSQINNVNLL
                     FDENIILRVVENIISNAFRYARNNVSILIYIEQELLTFVIEDDGIGFSKESLKSALRP
                     FYRDKTLNESNAHFGMGLYISKILCEKHGGSISIENNLTNSAKITAKFSTKN"
     gene            complement(17303..17977)
                     /locus_tag="PC523_002131"
     CDS             complement(17303..17977)
                     /locus_tag="PC523_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2475652.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNSYEVYTAQSGKDALKKISK
                     NPDIILLDINIPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIDNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETIWGV
                     GYKWIK"
     gene            complement(18770..19597)
                     /locus_tag="PC523_002132"
     CDS             complement(18770..19597)
                     /locus_tag="PC523_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDF2475653.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGAKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGDLIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGVAICYDIRFPE
                     LSRLMALKGAEIVILPASFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGKIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            19979..20299
                     /gene="sugE"
                     /locus_tag="PC523_002133"
     CDS             19979..20299
                     /gene="sugE"
                     /locus_tag="PC523_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDF2475654.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            complement(20429..21109)
                     /locus_tag="PC523_002134"
     CDS             complement(20429..21109)
                     /locus_tag="PC523_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDF2475655.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSIKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            21505..22557
                     /locus_tag="PC523_002135"
     CDS             21505..22557
                     /locus_tag="PC523_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2475656.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGTEVKSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDRDGADIVIESAGTPMTCGQVLLLAKKGGTILYAGVPYGDVVLTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSTGLYHMKKGAMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIGN"
     gene            22603..23019
                     /locus_tag="PC523_002136"
     CDS             22603..23019
                     /locus_tag="PC523_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2475657.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAVIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLNL"
     gene            23048..23521
                     /locus_tag="PC523_002137"
     CDS             23048..23521
                     /locus_tag="PC523_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2475658.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDENLIVLVKYPKTVLGLVNNGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELIAKDVKCFIQIVPDAKKIDVGTLL"
     gene            23578..24336
                     /locus_tag="PC523_002138"
     CDS             23578..24336
                     /locus_tag="PC523_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2475659.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNENVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            24360..25199
                     /locus_tag="PC523_002139"
     CDS             24360..25199
                     /locus_tag="PC523_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2475660.1"
                     /translation="MSDIKNEVAKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAISGIV
                     GLF"
BASE COUNT         8935 a         3168 c         4179 g         9157 t
ORIGIN      
        1 ataacttatg taagaaccgt aggaactatg gggatagcct gtggagaatt agtaagacat
       61 attttagtat gcaaaattct atgaagcagg aactctgtga ctttagtcat tggaggttca
      121 ggagaaagtc ttattatcat catgaaaaat cgcataaagt ggcgttaacg tgttccaata
      181 tccaagttca tcttcatcat atgcgatttt tagttctcta tatcgattta agcaagtagt
      241 tattgtgtat aaatacgctg gataaaattt aatatttctt tttttcgttt ccttaagtaa
      301 attagtaata tctatttcta tagtcaaatt aaaccctaca tttgtcattt ttgaaaagta
      361 gtaaaaatgt gtatatcgtt cccagttttg taaatcaatt ttatagaatt tttgtttatt
      421 catataaaat caccatgtcc tttataatct ttatttgatt atcaatgtct ttatttgtaa
      481 gtttcaaaat ggaagcagat gttttcatgc tatcaaataa gaacattatg tgtattgcaa
      541 ctgatttaga atcacatttt ttaaattcac ttttagattg tccataatcg attaaatttg
      601 aaagcattgt gatagctgaa ctgtatcttt tattcatata atcacgttgg tctttttcta
      661 taaaagcaaa ttcgtgtatt gcaaaatcaa atccacagta tttgccattt atgtcagcct
      721 tttgttgttg gaaaaaatat tctagcatat gcttagctga tatattttcc ttgatacctt
      781 tatccaataa attttgagag tagtgtttat gagtatttaa tatactgata aaaatttctt
      841 ttggtgaatt aaagtgtcta tatagaccac cacggcttaa attacatgct tcacatatat
      901 ctttcattgt cacattacta tatccttttt cagaaaatag cattattgat gtatcaatga
      961 taaattgttt agtttgttct ccttttgttg gcatatgttt taacctcctt tttaacgaca
     1021 caagtgtcac ttttaattat gagacatttg tgtcgttttg tcaagttaaa gttatatact
     1081 ataaaataat tacaatacaa taaggttaat ttataaaata gttgaagaat acaaaagaat
     1141 ataatggaac tgatttattt ttaagttgat gtaaaaaata attaaataat aatattatta
     1201 ttgactttta aaacttacat gtgtaatata taaattacta tcaattatac tgcttacata
     1261 tttttatgag atactaaaaa acataataaa aacatagatt agttacgtct ataagattac
     1321 tttaacctta aagcttacgt atgtaatata ttaaatgata aaggtggttt atatgagaaa
     1381 aaataaaaaa gcaagaaaaa tgataatttt tggttttcta atgtgtctac ttatagttat
     1441 tattgttttt ttagcaaaat tcatactaga tggactcaat aaaaacaaac cagatgaagt
     1501 gtttaagcag tatatgtctt ttgcgaataa aaaacaatat gagaaaatgt atgatttatt
     1561 agatgaaaag agcaagtctg aaaataaaaa agaagatttt gttttaagaa ataaaaaaat
     1621 ctatgaagga attgatgcgc acaatataag tattaagatt aatgatatta aaaaaaataa
     1681 aaacaatgat aaattaataa actatgattc taagatggat actttagcag gtgaaatttt
     1741 attctcaaat gaagtactat taactagaga tagacagaaa aattataaaa taaaatggca
     1801 gtcaagcgtc atatttccag aacttgaaga agataacact gtaagggtgt caaagctgaa
     1861 aggtaagaga ggacgtatct tagatagaaa tggtgtgatg attgctggtc aaggtcttgc
     1921 atctatgata ggattagtcc ctggaaaaat gagtgataat atagaagact taaaaaaatt
     1981 atctacctta ctaaatgtat cagttgaaca aattgaaaag aaattaaatg catcttgggt
     2041 aaaagaaaat tcaatggtac ctataaaaac tattgaaaaa ataaaagaaa atccggatgg
     2101 tactgtaaaa gaagaagata aggaactaca agagtcgctt cttagtattc ctggagttaa
     2161 aatatctaat acagaggtta gagtatatcc atttggtgaa aaaactggtc acttaactgg
     2221 ttatgttcaa aatgtaaatg cagagatact aaaggagaaa gaaggtaaga ggtacaatgc
     2281 taatagtata attggtaaga ttggtttaga aagtttactg gaagacagaa tacgtggtat
     2341 tgatggatat gagattatca tagctgatag atatggtgac aagaaagaaa ctttagtaac
     2401 tgagccaaag gtagatggag aagatgtaaa gctaactatg gattcaaaac tacagagtaa
     2461 actatatgac caaatgaaaa atgataaagg ttgtgcagta gttatgaatc ctaaaacagg
     2521 agaggtttta tcacttgtaa gtagtccgtc gtacaatcca aatgagttta tattgggaat
     2581 gtcagaagat agatggaatg aactcaataa aaatgaaaat aaaccaatgt acaatagatt
     2641 taaagcacga ttatgtcctg ggtcttcttt taaaccagtt actgctggta ttggtataac
     2701 aacaggaaag attaatccaa atgaaaattt tggacacagc ggtctaagtt ggcaaaaaga
     2761 ttctagttgg ggaagctata aggtaaccac gttaaaagat tatggaaata ctgtaaatat
     2821 ggaaaatgct ttgatttatt ctgataatat ttattttgct aaggcagcct taaaaatagg
     2881 tgaagatgta ttgtcaaaag agttgttgaa gcttggattt gatgaaagta tgccatttga
     2941 atttgggtta tcctcttcaa aatttggtac tgacaataaa tttgagactg aaatacaatt
     3001 agcagatact ggttatggac agggtaagct tcttgtaaat ccagtacata tggcatcaat
     3061 atattcagcc tttgtaaatg atggagacat gataaaacct catcttgaat ttaaaaagaa
     3121 tttacaggct gaattttgga agaaaggtgt gttttctaaa gaagctgtaa aaattattcg
     3181 tgatgaccta cttcaagttg ttgaaagtcc taatggcaca ggtcatagcg ctatgattaa
     3241 aggaattaga attgctggga aaactggtac agcagaaatt aaagcatcaa aagatgatgt
     3301 tacaggtact gaacttgggt ggtttaatgc ttttactgta gatggaaata atgataaaca
     3361 atatctagca attgctatga ttgaagatgt aaagagtcgt gggggaagtc actatgttat
     3421 gcctattgtt aaatcagtat ttgaagatta aatttactca atttgaaaat taatcttatt
     3481 cagttgagaa taatatttta gataaattgt ttttagactt tttagtaaat tctaaaacaa
     3541 ttaaagaggc tgtttcaaaa tagagatatg cctctttttc attgtttaaa tgataaaaat
     3601 atattttact taatacaaaa aattaaatag tggcaacaat tgaggatgtg agggagagtt
     3661 gccactattt gtatagggta ttatattatg tttttaagct agttttacta tcttttatgt
     3721 ttatataata agccttgtat atgaatttaa tagataatta atatgaaaat tatgtgaatt
     3781 ttttagaatg tatagtaaat atattaggga ttatagattt ttttgtcatt taatatagat
     3841 aatgttatct tagatgctga aataccatta gaataatctt tgctttgaat atttgtagca
     3901 aagaaatagg tattttcatt tttttctaca tagcctataa accaaccatt cacatctttt
     3961 ccatttatat tagcagtacc tgttttacca gaaagtgata ccccatcttt ttttgatatt
     4021 aacaatgaat cttttacagt ttctatattt ttatcttcaa aattaaactt atttgtataa
     4081 aaactttcaa gtaattctac ctgttcaata ggtgatattt ttaatgaaga ttctagccaa
     4141 aagtttgaga taccacctga aacatcataa ttaccatatc caatttgctc aagataagtt
     4201 tgtatgtttt ttatgcctgt ttttttatct agagtctgaa agtaccagtt tacggaattt
     4261 tccatagcag aaaataaatc ttggtcttca ttccaagaaa tattagaatg atgttttcca
     4321 tcccaaggta tatgtgaatt atctcttgtt attatcccat tttccaatcc caataatgca
     4381 ctataaatct tataggtaga atttggagaa attcttgttt tacttttttc tttattgtaa
     4441 atatgatatt gcttggattt taaatcatac ataacaaaac ttccatcaaa acctttaaaa
     4501 tacttactta aatcctcata catagtttgt ttatttgtag gtctatattt ttcgttgata
     4561 tctgccataa tagatagttt aggaatattt ccaaatacaa ttaaagctat caatgtaaaa
     4621 attaaaatgc ttttgaattt taaagtattt gattcattat gaaaatccat tatcttcaat
     4681 agtcgttttt ttatttgttt ttttgaccca ccaatatctg atgtaagtgt aagatttgat
     4741 ggtcttgaaa tattatctac aaagtttatt agagtttgtc catattctat atgattatct
     4801 ttatcaagct tttttaaaac tgaaatatca catgcaattt cacggtctat acgcatttct
     4861 ttaaatgcat accatacaag aggattaaac caatataata tttgaaaaaa gcacattata
     4921 taatttatca gcatatctct atttttaaaa tgttgcaatt catgaagtaa gatatatttt
     4981 atatctttta aagataattt agatatagac ttatctggta atataatgtg tggtttaaag
     5041 tatccaaatg taataggcgt atttataata ggtgatttgc atagtataaa atccttattt
     5101 ataccaatat cttttttaca cttttcaaat ataaaaacta tctcctcatc tcttaaaatt
     5161 tgtattgatt ttttcatact ttttatttta atgttgcaat gaatagtaat aactatcatg
     5221 attaaaattc caattatcca taatattagc aataaaatat ttaaaatatc aaaattaact
     5281 tggtttaggg atactgaaaa atcgtttaat gcatttgaat tgttaattat agaatttgga
     5341 ctatttttta tcatagaagt cctattagta ttgcttccaa gttgattaaa agtatataaa
     5401 tagttaaata catttccaag attaatatat ttaagaggga caaatggaat tattaacatt
     5461 ataagcaata taaatccaat attgtattgg tactttgcag aaatatgttt tttaaatact
     5521 tttttggcaa gtaaaattac aacaattaat attgataagg ttaatgtact gagtaaaaat
     5581 cgtataaaga atggggcata catatttatt tatccccctt agtcgtcctt ttatttaaga
     5641 tttctctcag ttcctcaata tcatcttcag atagtttatc attttctaaa aagtttagta
     5701 ccatagaatt aagagtgcca ttataaaaac gatttaaaaa agaactactt tctgtagcta
     5761 cgtattcttc ttctttaaca agtggagtat aaacgaatac tctgctattt ttttcatatg
     5821 ttaaagctcc ttttttaact aatctcaata acattgtttg aattgtttta ggactccatg
     5881 tacttgtttc taccagcttt tcaattactt cagtagtgct gataggagca tatttccaaa
     5941 taattttcat aacttgatat tctgcttctg aaatatgtgg taactttttc atactatacc
     6001 tccttctact tacatttgta atacataaag tatattatca ttaatttagt ttgtcaattg
     6061 ttaaaaataa ttcatttata gttgattaaa taaacgtata tagttaaata aaatttttat
     6121 ttaagctgtg caagtatagc tttaaactaa ataaaaggtt tatattgata gctaaaaata
     6181 tagttgataa aaagccaaag tggggtaatg agtgtgaaag atatattttt agaattatgt
     6241 taatagtata tagttatatt aatagcatat agttacacta atataaaata aaagtgcctt
     6301 ataaatagaa ttttttgagg cacttattat ttgtctaaat tagtttttat tattttgcaa
     6361 tctcaaccgt ttataatctc gaacattttt tttatatctt ctgatgccat ttcaaatttt
     6421 gatttttcaa tcagtatgtt tccaccagtc ataatatatg atggaaatac tcttaaatta
     6481 tcataataaa gttcattatt acgaagttta tatgttgaaa tggaagggat gacattcttt
     6541 tttatatgtt ttttagtaat catattaaaa gatttctttg caacatctcc cattaacatt
     6601 actacctcta ggtttttaaa caatttaatt tcttcttcta aaataggcat atgtaattta
     6661 attgtatcac gagttattgt atattctgat ttaggaagtt ttacggcatt agttatatag
     6721 atacccatat ctaggatatc atttatatta gatacttcta ttcctgcatt gttaaataaa
     6781 ggaatagtag tttttaaata atcagcatct ttatctatac tataaaaatc gtcattggtg
     6841 ttagttggga caacttcatt aatcattata gtttttattt tgtctgtgtc tattgttata
     6901 ttaggaaggt aggatattat tctgttgtca tcaaaaagat tattctgttt aataaattca
     6961 tttaatgttg attttatatt tattttcata taacgagtct ccttagtttt aagctttaaa
     7021 ttactataaa agtatagttt taaggtatat aattttgaaa atatagtaac atatttgaaa
     7081 gtataataac atataaaaca tgacaacaga atgtcatgtt ttatcaataa tctatcgcaa
     7141 cttagaattt attagagctt ctgcctgttc ataatcgtct ttatgaacat aaattaagta
     7201 ctcataagag aaatcttctt tttgacctaa attgccaagg gttatttcat tataaaaagg
     7261 ttttttagag taagtattat ttttcacttt atacttatat ttaattttat ttactgataa
     7321 tatattgcat atctcgttaa atctatctaa agaatatcct cggaaaagct cagttctatt
     7381 taaaatcata aggaaaacct ccttttaaaa gtaatatatt gaaaagcata ggagaaatgt
     7441 actataagtt gttataaata aaaggtagca aatttaaatt gatttgtaaa gtttttatga
     7501 gtattatatt agtgataaaa caaataattt ttagaatcta actaataaca atttaaaata
     7561 gataacttat gtaagaacca taggaactat gggggatatc ctgtggagaa ttagtaagac
     7621 atattttagt atgcaaaatt ctatgaatta ggaaccttgt gactttagtc atggaggttc
     7681 agaaggatat ttaagtattg aaaggagagt ttaattgaaa aaatatctca agaaaaaata
     7741 agtacactta ctcaagaagt atcacaagaa aaaagtgagc agtggaaata tgtatatgaa
     7801 aaataatgat gaaataactg cttttaaagg attagataaa tatgatataa ataattattg
     7861 tttagtagca gactatatta gatgtctaaa gaaatataaa ttaatagatt agtgtttgat
     7921 taataaatta gtgcttgatt taaggattat agagtaaatt tgaagttagt gtgataatga
     7981 aactaagctt aaatctaata taactgtaaa acataaactt aaatagagtt gccaaaataa
     8041 aattggtaac tctatttttt attttaattt ttattaaaat tattaggtgt tatagaattg
     8101 ttattttttc atgttagtat tctcttagta atagatttaa agtattacaa gaagggagaa
     8161 ataaatatga aagatttaat tattgaaaca aaaaacttat caaaaagata tggagaacaa
     8221 atggcagtta acaatgtttc tctacaaatt gaacgaaata gtatatatgg acttttagga
     8281 ccaaatggtg caggaaagtc cacaacactt aaaatgcttg ttggtcttct tcgcccaaca
     8341 gatggacgta ttatttttga tagaaagcct tggaaacgag aatctcttga aaaaataggt
     8401 tctttaatag aatctcctgc attgtatgga aaccttacag cagaggaaaa tttgttagta
     8461 catacaaaaa tattgggaat acctaaagat aaaatttatg aggtgttgga aatagtaaat
     8521 ttgaaaaata caggtaaaaa gagagcttca caattttcta tgggtatgaa acaaaggtta
     8581 ggaattgcta ttgcactttt gaatgaccca gagttgttaa tactagatga gccaactaat
     8641 ggtcttgacc cctttggcat tcaagaattg cgtgaattga ttgcttcttt tccagaaaaa
     8701 ggtataactg taattctttc tagccatatt ctatcagagg ttgctcaagt tgtagataat
     8761 ataggaatta taaatggagg aagactacta tttcaaggaa tgcctgaccc taatgaaaat
     8821 ctagaagaat tttttacaga tgttattctt aaaggaggac aaaacagatg aatgctttac
     8881 aatcagaact tctaaagtat aaaagaacct ttatgggtaa acttattgtt tttttcccag
     8941 tatcttttgc agcatatgca tttataatgc aatcaacact tatgcaaaat ccactatctc
     9001 aaataaattc ttgggcatgg gaaagtcttt tagcattgat ttttaactgg tggtcaatga
     9061 tgtttctgtc tattggattt gcattgtttg ctactttggt agcttcccag gaaaaaaaag
     9121 ctggaaatta tcgtgcttta cgtacacaca atgtgtctcc catgacaatt tggattaata
     9181 aaattatagc tatggcagtt tacagtttta tttcaacgct agttttaata gtcgtaacaa
     9241 ttatgactgg tttaatattg aaagctggac caattccttt tggacagatt ataggggcaa
     9301 gtattgtatg ttgggttggt tctcttgcta tacttccaat ccagctatgg ctagctacat
     9361 ggaaaggtat gttcctaagt atgggagttg gagctttagg catgatattt ggtgtccttg
     9421 ctgcgtcaaa accattttgg gttgcagtac cttggagttg ggcagttaga atggtatgcc
     9481 caatcatcaa catacatcct aatggtacta tattagaagc tggagaccca cttttgaata
     9541 cttcagtgat tcctattggg attgttgttt ctttggttgt gtttatagta ttaacagcac
     9601 ttactgcagc ttggttcaat aggagggatg acaaatgata aaactattat catcagaatg
     9661 gcttagagca aagaggacgg cagtccaagg tcttactctt tatatgccta ttattttttc
     9721 attatgtgtg gttgcttatc tcacaattaa atctggaagt acacaagaat ttgcttttga
     9781 aggatttttt acaatatgga ctgtattcat cattcctatt ggagtaggta ttttagcagg
     9841 atttatagtg caggaggaag agcttgctgg aaattttaat ggttttttat gtgtaggtat
     9901 ttctcgtgtt agattatatc tgggaaaatt tctttttctt ctgttttgcc taactatatg
     9961 cacatttatt gctacgttga tactctgcat aggaatgaat attgcagtgc catctggagc
    10021 aacaatatgg ttattcctgt cagcagcagg acttgtggtg attgggactc ttccactatt
    10081 agctattcat ctttgggtga gttttgcatg gggtatgggt gcgtctattg gtattagtat
    10141 tggtggaatt ttgatggcag caatacttgg attaactagc gttggtgcaa aagtctgggc
    10201 atttgttccc tggacatggc cagtaaaact aggtatgctt ccaggtactt attttattaa
    10261 agaagctgga actatttcaa ctgaggtatt ttactctaaa atgatgcaat cagctactgt
    10321 agtatttata gtagtagcaa taggtttgat tatattcttg ataggaggcg ttatttggtt
    10381 taaaatatgg gagggaagga aaagttacga atagacgaat attgttttaa tactaacctt
    10441 cccataagag aaaggtggtg ttatattgag taaaatattg attattgatg atgaaataga
    10501 tttagtaatg ctacttgagg acgaattgaa ggcaaaaggg catgaagtgc taatagctta
    10561 tgatggtcag gcaggtatag aactatcaaa aaatgagcct gacctcatta tactggatat
    10621 tatgatgcct aaaatggatg ggtttgaggt atgccaggca attagagatg atgttttgtg
    10681 cccaattata ttcttaagtg caaaacaatc agaaaccgac aaaatcaaag ggttgacctt
    10741 tggaggggat gattatgtaa caaagccatt tggattgcgt gaacttatgg caaggattga
    10801 agctaatctg cgtagggaaa agcgttcaca gtatatcaat gaggagaaca agaggtctac
    10861 tttgtatttt gaaaagttat gcttatatat gaaggaacgt gcaattaaaa ttgatggtaa
    10921 ggatattgat ttaacaaaaa tagagtatga tattgttgaa ttactagcac tccatgcagg
    10981 acaagttttc tcaagagaac aaatctatga aaaagtatgg gggtgtgatt ctgaaggtga
    11041 ttcatcaact gttgttgaac gagtcaaaaa aatacgagct aagttttctg ctgtgacacc
    11101 agaaaaagaa tacatttcca cagtttgggg gattgggtat aagtggaata aaatgtaaag
    11161 ggagagaggt cattaaatga aaaatcaatc acttgccacc caattcaggc gtacttttat
    11221 ttttattata atagctagta tagttgccac agtgattact tatgtatttg ctttttattt
    11281 gtttatttat tccttgaata aggatattta tccaccaaac tattatgagc gacaagttcc
    11341 tagaattaaa aaatacattg atgaaaaaaa catagctttg ttatcacaat caaatgaaga
    11401 aggtttaaaa agaactattc gcggggatgg tatgttatat caagttgtgg atgataatgg
    11461 caatattcta tatggtaata accctaaaaa accatttaaa acaaaagaag aattatttaa
    11521 taattttata aatacaactg ttcgtaaagg tggttatatt tatactgttc caattaaagg
    11581 tactaatgga aaaattgagg gagctgttat tctattctac caagtgaaaa tgacctttgc
    11641 aaatagtatg gggaggttga cgtttgcagt tattgtaata gctatgtttt ctccatttct
    11701 atatgttata ggttttacta tatggctttc taagcagttt gtaaagaata tcaatcaacc
    11761 attacatcta ttgattgatg catccaagaa gataaaagaa aaagaccttg attttgaaat
    11821 cgactattat tcagataatg aactcggtaa actttgtagt gccttttctg aaatgaaaga
    11881 cgaattaaaa aactctttat ctgcacagtg gaagatggag caggaacgtg ttgaaatggt
    11941 ggaagctcta gcacatgatt taaaatctcc tctttctatt gttttgggat atacggatgc
    12001 acttattggt aataatacag atgacaatga aaaacttcat aggtatctta cagtgataag
    12061 agaaaatacg gaaaaaagtg ctgctcttgt ccaaaagatg caatatacgt ctgatttaga
    12121 aaagtcaaat atacaattaa atcctgttcc tataaattta cctgaatttt tggtgcaaaa
    12181 ggtacaagac tatgaattac aggctcatca aaaggacgtt gaacttatat tgaaaatgta
    12241 tggtaatata caatcaccaa ttcaaataga cttagataga ttgacacgaa tttttgataa
    12301 tatcatatct aacagtctgc aatacactcc gtcaggtggt aatattagta ttattgcaaa
    12361 ggctgaaaaa aattgtattt cttatgagat ttgtgattct ggcagtgggt ttagctctaa
    12421 agatttaaaa aaggctcttg ataaatttta tcgaggagat gaagctagac agactaaagg
    12481 tgggcattct ggtcttgggc tttacattgt caaacaatta gtggaacagc ttggagggtc
    12541 tgttaaaata gaaaattcaa aatcaggtgg agcatgtgtg aaattttggc atagcattta
    12601 actatatatt ttaaattaaa ggggagtgtc tcagaacgaa atttttagtt cgtgaggcac
    12661 tctttttttg tgaagttaaa tatattttca tcattccaac aatgaaaaaa ggtttatctc
    12721 tattttgaga caggcctttt tttaaaactt atattatagt tggaaaggtt tattagttag
    12781 atttgaaaga aaatggagac agtcattgat aatctgattt atattattat ctgtaaaata
    12841 gttataaatt gatatctata cagtcaatta tattcatttt tttaactttt cttaatggaa
    12901 taagagtagc tataatacat gcaagtatag aaataatagt agcttgtaaa attgaagtaa
    12961 ttggtatatt agtaaaatca agcttttcta tagttgccat gtttataata atagcactag
    13021 catacccagc aattgaacca agtatagcag caaaaattcc ataataaaca ccttcccata
    13081 aaaacatctt atataagcta ctattactca taccaatagc tcttttaact ccaatctcat
    13141 tagtcctagt atttatattt gtatgagttg tgttaatgat gtttaaagta ccaatcaatc
    13201 ccacaaataa tataactgcc catgctagaa gttctatttg tttaaaactc tctttaagtt
    13261 gcttatctga ttcaatatat gatatccaat gagagccagg attattttca caaatctgtt
    13321 caatagacaa tcttatttgt tctaaatctg atttatcttt agcataaata tttatctggt
    13381 tgaacttgtt tttatttgtc aaggtgttat aaacagaatc atatacaata atatcaacac
    13441 catttaccca gccattacct tgtaaaaaga acattttatc taccgtacta tatatatcta
    13501 gttgtttttt gttaattgtg atattatctt taggttttaa atttgtatat ttagttttta
    13561 cacctggtgt agaaatagca agtggatttt ttataaaaca gatagaacca tttttaaagt
    13621 cttctaatgt agaatctgta atagaaggca ttaagtcttt taaagtttgt tcatcaattc
    13681 caatgatttt taacatctca ccactatttt caaaatttat atctgtatct atttcgccct
    13741 gtttgtattc agagtacttt ataaaagaag tgcgattgac gtttttcatg ttatctattt
    13801 tatctagtat tgacttatca aaaccagata tttcatttgt taaggaaaaa tctccttctt
    13861 ttaaattact tactgaccta gatacatcta atacattaga gaactcactc agtgcaacga
    13921 atactgttat actcataaat aatgatagta tggttattat agttcttcct ttatttcttt
    13981 ttaagtttag ccatgctaga tgtctttcaa agttttttat tgttttttct cgtttaattt
    14041 ttcttttgat tttggtagta ctgccagcca tagcaatttt gggagagaca tgtgaagcat
    14101 atattgctga tggtaatgct gaaataaatg aaaaaatcaa agatatacta gtgcttaata
    14161 ctaatgggaa catatatgct gttgtgtttt tatgtataag ctcagtaatt tctttattat
    14221 catttgctag aagtatatct ggatttaaga tattagtgac cattccagtt atgcctttag
    14281 aagatataat tccaattaca gctccaatag gtatggcaat agtacataaa attaagattt
    14341 gtaaaataac tatcttatat atctggtttg gttcagctcc tattcctctt aaacatccat
    14401 actctttaat tttctttgta acagagattt ttaaaatatt gtagattaca agtccagaag
    14461 ccagtaaaac caataaggca acaaataaag ataccaatat aatcatagat ataccatcac
    14521 tattagaact attttcatct ttttcaaact gtattttgag agcatttaag taaatccaat
    14581 tatattgaat tgaattgtga gaaagattta tttttttagc taattgattt actatttctt
    14641 gaaacttctc ttgctgtttt attttaaaat ccaaagagga taaaatatat ctcttaggta
    14701 atagattttc tgatgcacct ttgcccacaa taccgtttac tataccactt acgtatcctg
    14761 tataatcatc ctccaaaata ccactcaatt taaagttagc tgtgtaatta tatggtggta
    14821 ttgtatcatt taaaagtgaa atatctaaat tcataggtat agtcactcct agtcttggtt
    14881 ttatacctat aagctttagt gtgttttcat ctaaggctat ttcgttttta tctttaggca
    14941 aatgtccaga ttttaatttc attaatttgg ggtagttaga aagtcctgtt ttatcatact
    15001 cttttacaag tacagagata ccagaatcct ttattttact actgccaaga gttattgaag
    15061 taccagcttg ataaactcta tcatctttct ttatgtcatc aacttgattt ttagtgagtt
    15121 ggtgaaagga tacatgtcta tcaccattaa aagaacgggc ttgttcaatt cttaaatttt
    15181 gtaggatacc aattgactgt ccaaccacag ttgtcataat agttgatagt actattgcta
    15241 taatgatgaa tgtagtcata agtttctggc tttttaattc ttttattgaa agtgaaaggt
    15301 aattcttcat ttctatttca cctccataag aacaccatca ataattgaaa tttttctatc
    15361 agcaagtttt gcaattctat catcatgagt aattaccact aaagtctttc caaactgttt
    15421 tgaagttttt ataagaagat tcataacttc actgccactt ttactatcca agtttcctgt
    15481 aggctcatca gcaaatatta tatctggttt tgcaataagt gctctagcaa ttgcaactct
    15541 ttgttgctga ccacctgaaa gttcatgggg aagatgattg agtctatctg caattccaag
    15601 taatgatgaa atttcattta aatagtcttt atcaggttgt tttttatcta gaagtacagg
    15661 cattgaaata ttttcagatg ctgtaagaac aggtaaaaga ttaaactgtt gaaaaacgaa
    15721 tccaaaggat tttcttctaa gtgcagatag ttctttttca ctgtgtttat atatatcttt
    15781 atcatcaagt aacacttgtc cagaggtagg attatctaat ccacttaaaa tatgtaaaag
    15841 tgtgcttttt ccacttccag aaggtccaat tatagaaaca aattcacctg agtttatttc
    15901 taaatttaca ttattaagtg cgataacttt attgttatta gaaccatata tttttgataa
    15961 ttgttttgct tttataatca taaaaaacac tctccttttc tatataagaa gagtgtaagt
    16021 tttaaaactc aattttttct caacttttta attttgttta ctaaaaattt atttcaaaaa
    16081 aacactttgt aaactctaag ttataaagtt tataaaagag gagtgaaagc ctaaaaaatt
    16141 gatttaatat tgctttaatt ttttgttgag aatttagcag taattttagc actgttagtc
    16201 aaattatttt caatagaaat agagccacca tgtttttcac ataatatttt tgaaatataa
    16261 agacccatac caaagtgtgc attgctttca tttaaagtct tatctctata aaaaggtctt
    16321 aaagctgatt ttagagattc tttgctaaat cctataccat catcttcaat tacaaaggta
    16381 agaagttctt gttcaatata tataagtatg gatacattat ttctagcata tctaaaagca
    16441 ttcgatatta tattttcgac gactctaaga atgatatttt catcaaacaa tagatttaca
    16501 ttatttatct gacttttaag tgtaaaagat tttccttctt gtttagcaat gatagatata
    16561 ttttcatcta agttttttat aaacttgttt atatttgaca tagattttga aacaactaag
    16621 tcttctaatc gttgggcatt actcatattt tctacatagt attcaattct gttaatatgc
    16681 tcagacataa gctgtgtggt tgataaaatc ttctcatcac taagcttgcc tgttggtatg
    16741 tattttgtta aatagtttga gtaacctttt agaactgtta ggggatttct taaatcatga
    16801 gaaaaagcag cattaagttg ttttctatca ttaatcatag tccacatctt tatgttgttt
    16861 ttatttagtt cagacctcat tttttcaaaa gcattacata gattacctaa ttcatcatta
    16921 ttattatatt ctaaacaaaa gtctaaattg ttgttggata tttccattgc acctttattg
    16981 agtatttcta tgggtgtttt tagttttacc ttgtaaaaaa ttatatcagc taataataaa
    17041 agtcctataa aaaatataag tggaagtaca agctgaagta tgttgattat attaagtaac
    17101 gttttatcat aatcactaaa ttggttcaca ttgttttgaa ctatggttat aaaacccgta
    17161 tttataggtg tttttaaata cttatcttgt atataattat atagattatt aagcaaaatg
    17221 tatgagaaag tagtaagtat tgatgctata attagtgaag ataaagtaag aacaaagaaa
    17281 acttttttta atgacatatt atttatttta tccatttata accaactccc cagattgttt
    17341 ctatataatt ttcaagagta tgttttgata gtttgattcg tatctttcta atgtgttcca
    17401 ttataacatc actatttcca ttgctattgt atccccaaac tctatcatag attttttctt
    17461 tatcaaatac ttgtccagga ttcatagaga gaagttctac tatatcaaat tccttttttg
    17521 aaagtgaaat ctgattgtta tctatacaaa ttaacctttt ttcgtaatct atcgttaaat
    17581 cattataaaa tttaatttta gtttgatttg acttcctttg ttcacgtctt aaatgggcag
    17641 atactctagc tccaagttcg tctaaatcaa aaggttttac aatataatca tcagcaccag
    17701 cacgaaaacc atttatcttg tcagtatttt caattctagc tgtaagaaat agaatagggc
    17761 atgatacata atctctaatt ctattacaaa cttcaattcc atctatttca gggatgttta
    17821 tatcaagtag aataatatct ggatttttgg atattttttt taatgcgtct tttccacttt
    17881 gagcagtgta aacctcataa ctattcattt caaagtaatc ttttaagagt gttattatac
    17941 cttcctcatc atctacgacg agtattttat tgttcataag aatgctccct tctaaattaa
    18001 gtttgtatag tatgtatact ttagtataaa aatctcaata aattctcaaa ttcttagtta
    18061 tatttcaaaa cttaatcatt aaagttagta tatttttata tgttaaataa atcaaggatg
    18121 aaattattag aaattttaat agaatatata tttgatattt aatacttatt ggaatgcaaa
    18181 aacacagctg atgaaatctt ataatttaag tttaaaatgg ataaatagaa aggaaggatt
    18241 taattaaaaa ggttaaaaag tatataaatt ataaaaatga attaatctac attaattagt
    18301 tgatatatca attattattg tttttttcag ttgcttaaaa catactcgat atatcaacga
    18361 atgttgtttt ttaattaaaa gaaattttag gagagataat atcaacattg aataaggtag
    18421 agcaagattt ttacataatt gtgaacattg tctggcatgt atgtattggt gttttaagca
    18481 agtaattcaa tagaaagatg tgactaaaag tagaaagaga tgccataatc caaatgtaaa
    18541 gctagaagac ataataagta aaaaaagtaa atatatttaa taaaagaaat aactaatagt
    18601 gattaaatta gtaaatactg ataattagca taatcattag tattattagt attatttaat
    18661 taaattaata ttagtattat ttttatgaaa ttaattttaa atcaaattaa tgagaacccc
    18721 ataaattttt ttaacactta atttatgatt tatggggtta tgttctgttt tatttcaaag
    18781 tattaagttc atataaatca gttcttctgt gtttaagaag tggtaactgt tgtcttatgt
    18841 caggaattaa atctctatca atgtcagcat aaataataca ttctttttca tcagcttgag
    18901 caatgatttt tccccaaggg tctgaaattc ttgaattgcc aaaagctatg taggaggcat
    18961 tcatatttct agcaggtgca gcaccaacat agaatatctg attatctaaa gcacgcattc
    19021 ttatagataa ctcccaatgg gcaggaccag tagtcatatt aaatgaagca ggtaagataa
    19081 cgatttcagc acctttaaga gccattaaac gtgataactc agggaaacga atatcataac
    19141 aaatagctac accaactttt ccatattcag tatctattac agtgacatca tttccagctg
    19201 taagagtatc tgattcttta aaactaactt ttcctgtaac ttcaatgtca aataagtgca
    19261 ttttacgatg ttttccaatt agatctccat ttttatcaaa tacatagcaa gtattataga
    19321 tttttccatc ttctaactca ggaatactac cagaaactaa ataaattcca tgtttctcag
    19381 ctaatttact caatatcgtt gttgtttcac ctggatattc ttcagcaaat ttaggaaaat
    19441 atttattttc atatgggcag ttaaacattt ctggcaaaac tacaatttta gctccattga
    19501 tggcagcttc tgttaccata tctacagcat gattgatatt ttcaatttta ttttctgtag
    19561 taatcatctg gcatactgca attttataga aattcataga aattactcct ttcaatattt
    19621 ttatgttatt ataacatatt aaataaaaat tagatattct tagattgacc taaatgggta
    19681 tggagagaag tgaataagaa tattaaaagg tatataaaag ttatttttat tatagatgga
    19741 acccatatat tgactttttt tatatatgtt gatagtatta ttatagataa aatagaataa
    19801 ttaatagagt tttatagggt tccgcaatta ttaatgattg gtctggtccg agataaaacc
    19861 cacaacatag tttttgttgt gaacacggaa ggacaaaaac ctgggagata ttttataata
    19921 tttctcaggt tattttattg gaaaaataag agatatattt gaaaagaggg atgagtttat
    19981 gaaatggtta tttttatttg ttgcaggttt gtttgaagtg ttttgggctt ttcaactaaa
    20041 aaattcacat ggattttcta aattaactcc aagtatactt actgttgttg gaatgattgt
    20101 aagtttttat tttttgtcat tagcactaaa acatcttcca cttggaactt catatgcaat
    20161 atggactgga attggtactg ttggtacagc tattgtaggc atggctatct taaatgagcc
    20221 tattagtata tcaagaattg catgtatagg ttttattgtg gtgggaattg tagggttaaa
    20281 gttattgtct accaattaga gtataattta aataagctat gaaattaaag gcgagaaaat
    20341 gtttaaaaat aatcaacatt taaaaagtct ttacttcata gcttatctta tttataagtt
    20401 tattatattt gaaatttata tgtatatact atttatctaa taaaagagca gttaaatcca
    20461 tagggtcaac tattacacca tctttataaa cacgcatatt tccagatgct atttcatcta
    20521 ttaagattac ttcatcattg tagaatccaa attcaaattt gatatcataa agttcaagac
    20581 ctttttcagc tatagtatca cgaactatgt tagaaatttt ttgagtcatg cttttcattg
    20641 aatcaaattg agcttgagtc ataatgttta aagcttctaa accttcagat gttataagag
    20701 ggtcacaacg gtcatcatct tttaaagttg cttctacgta acaatctagt ttagctcctt
    20761 cttcaacaca tgaaccataa cgacgtaaga aactaccaac agctctataa cgacaaataa
    20821 cttctaatcc attaccaaaa acttttgctg gtagaacttc catagttgcc tcttctaaat
    20881 tagcacttac ataatgagtt ttaattccag cattgtttag aatttcaaag aacttaatag
    20941 atgtctctaa attagctcta ccaattccat ctatacttag acctacacta ttttcgcctg
    21001 ggtcaaatac tccgtcttta ccagttacat cgtctttaaa tttaagtaag taatttccat
    21061 tatctaattc aaaaacatct ttagtctttc cttgatacac ttttttcata aacaacctcc
    21121 aaaaagttaa attattatat tgaataaggc attagtgtca aaaaagttct gatattcgta
    21181 tgaaaattta tattgatacc ttatatttaa tattatatca caaatagagg gggaatttcc
    21241 gaacaatttt ataaaaataa caaaaaaagt acattttata aaacttataa taggagaact
    21301 agtataaaat gtgagtgtcg tttatattga cacacataag ttttcgtata aaaaagtttt
    21361 gacacacttt gacacacatc taatgataat tttataaagt gcatgaaaac aatacttaca
    21421 tatattggca tgaaagttgc tttagatatt taacatataa aaaataagta tatattctca
    21481 aataaaaagt aggggtggtt aatcgtgaag tcagttagat tctatggtat aagagacaca
    21541 agagtggagg atgtagatgt accaaaaata ttagaaaaag atgatgtaat aattaaagta
    21601 aaagtagctg gaatatgtgg ctctgatata tcaaaatatt caaagacagg tccacatatg
    21661 gttggagaga ttttagggca tgaattttct ggagaagtag cacaggttgg tacagaggtg
    21721 aaaagcttta aaataggaga tagagttgct gtttgtccag ccatgccatg ttttgaatgt
    21781 gatgaatgta aaaaaggatt gtattcaaga tgtaataacg tagcaataat aggaaataaa
    21841 gaacttggag gttgttttgc agagtataca aaggtaaaag agagaaattt aataaaaata
    21901 ccagatgaga taagttatga aactgctgct gcactagagc cagtatgtat agcaggacat
    21961 ggtcttttta ggtcagaagc taaagttgga gatactgttg tagtacttgg aacaggtcca
    22021 ataggattat tttcaataca atgggcaaag atatttggct cgacaaaaat aatagctgtg
    22081 gatgtatttg atgagaagtt agacttggca aaagagttag gggcagatat ttgtataaat
    22141 gcaaaagaaa agaatattgt agaagaaata aaaaggttaa ctgatagaga tggtgcagat
    22201 attgttattg aatcggctgg tactccaatg acgtgtggtc aagtgttgtt gttagctaaa
    22261 aagggaggga caatattgta tgcaggtgta ccgtatgggg atgtagtttt gactagagaa
    22321 caatttgaaa agatagtaag aagtgaatta actgtaaagg gaacttggtt tggaaactcc
    22381 tttccatttc caggaaaaga atggagtact ggactgtatc atatgaaaaa gggtgctatg
    22441 aatgttgaaa aattagtaac acatagaata aatttagaag aagtaccagc gtattttgaa
    22501 aaagtatata aaagggatat tttctttggt aagataatga ttaatatagg taattagagt
    22561 actaaagaat aatccatata aaaaagttta gttaagaaag gtatgattca tatggcagga
    22621 ataattatag ctacacatgg aaatatggct aaagcgatgt tagaaagtgc tgaactaata
    22681 gtaggtaaac aggagaatgt agagacttta ggattaaacc atggtgatag catagatgag
    22741 tttagtagaa agttagaaga tggaattgag aaatataagg atgaaggagt tttagtattg
    22801 cttgattttt atggtgggac tccatttaat acatctgctg ttataataaa taaatttgct
    22861 aaggtatgtg agttagaatg tttgacaggt gtaaatttac ctatgctttt agaattattt
    22921 ttaaacagag atactatgaa gttgaaagac ttgaagaatt tatgtgagga agttgggata
    22981 tcaggaatca aagatgttaa aactgtttta aatttataga ataaattaaa aatctggagg
    23041 aattattatg agtaatatag cattaacaag aattgatgat agattaatac atgggcaagt
    23101 tattactgct tggtgtaaga ttacatcagc aaaaaggata gtaatcgtgg acgatttagt
    23161 agtaaaggac ccatttatag ttcaagtttt acaaatggca gctccatcaa ctgttaaggt
    23221 tgaagtacat gatgtagaat caggtgctga agttttaaaa tcacataatg gagatgaaaa
    23281 tttaatagta ttggtcaaat atcctaaaac tgttttaggt ttagtaaata atggggtaga
    23341 tttaaaggaa ttgaatgttg gaggtatggg agctggactt ggtagaaaaa gtttttataa
    23401 aaatatatct gtgtcagatg aagaaaaaga aatatttcga gagctaatag caaaggatgt
    23461 taaatgcttt atacaaatag taccagatgc aaagaaaatt gatgtaggaa cattattata
    23521 aagctttata aagaagatta tcttgtaaat ctttagaaga aaataaggag gaaaagtatg
    23581 catatttcac taattcaagc agtattgata gcaatttttt attatctttc ttggagtcca
    23641 tggttgactt atgttggatt ttttacatgg aatagacctc tattggctgg atttgtaacg
    23701 ggaattatat taggagaccc tgttcaagga gcaattatag gagcaggtat aaatatgata
    23761 tatctaggtt ttatatcagc aggaggagct cagatgggag acccagcttt tgcaggatat
    23821 gtaggcactg ctttagctat agcatctaag ctagatgtaa gtactgcaat ggcaatagct
    23881 gtaccattgg gaactgttgc aactgtactt tggattggaa agatgacagt aaattctttt
    23941 tttgcacatt gggctgatag agaggttcaa aaagggaatg ttgatagagt agcatttata
    24001 aatatagtac caccacagat actgttattt gcaatgagct ttataccagc actattagtt
    24061 gtttattttg gtccaaatgc gatagatggt atgttggaag taatgaatga gaatgtgtta
    24121 catgtattta atgtaattgg ggctatgttg ccagcacttg gaattgctat gaatttaaaa
    24181 ttgattggaa acaaatttac tatgcccttt ttcatattag gaatactaat ggctgtttat
    24241 tttaaagtag atataatagt aatatctgtt attggagtaa tacttgctct tacaattact
    24301 tcaattaagt acggaaaaga tagtgctaca tcttaaatac atgtgagggg gaaaatatta
    24361 tgtcagatat aaaaaatgaa gtagctaaga aaacgttaag caagaaagat gtaataaaat
    24421 cgtggttaag atggtttttc tttgcacaat ctaattacaa ttatgaaaga ttgcaatcaa
    24481 cagctttttc acattcaatg ttaccggtac ttaaaaagtt atatccagat aaggaagagc
    24541 taaaacaaga agtggagact catctagcat tttttaatac agagcctata tgtggatgtg
    24601 ttatacatgg aataacaata gctatggagg aagaaaaagc taatggagca gatgaaataa
    24661 gtggagatgg tatgaatgct atcaaaacag gcttgatggg tccactagca gggattggtg
    24721 atactttaac tcaaggagtt ataactccta tagtattagc tgtttgtatt ggtttaactg
    24781 agggaggagc atcaattgca gggcctatat tatttgtaat agctcaatat ataataatga
    24841 cttcaatttc ttttggaatg tggacgaatg gatataagta tgggaaaaaa gctgttgaaa
    24901 gtatactcca aggtggaata gtaaataagg ttatagaagg tgcttctata ctaggtactt
    24961 tagtcatggg gggacttgta ggtaggttta ttaatttgtc tacaccaata agttatacta
    25021 gtggagattt taaatttagc cttcaaacgg atttacttga taagatattt ccaggattaa
    25081 ttcctttggt acttacatta ttagtacttt ttgccttaaa gaaaggactt tctccaataa
    25141 aaataatgtt tatattaata gtagtgggag ctatttcagg tattgtggga ttattttaaa
    25201 taaatatata tgttggtgtg taaattatga ttctacatct attgattata tttgtaagat
    25261 gtaggaaaac ttatggtaaa aagtttagca attagggtaa ttttataata gattaaaatt
    25321 taagcttata taagctatat ttagtattta atattttaaa aagtatctca gaaagatttt
    25381 taatcagaat gaggtgcttt ttcattttca tataaaatat taagtaattt cacaaaaat
//
LOCUS       DAMJVM010000049        23685 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.050, whole genome shotgun sequence.
ACCESSION   DAMJVM010000049 DAMJVM010000000
VERSION     DAMJVM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23685
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.050"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            52..780
                     /locus_tag="PC523_002140"
                     /pseudo
     CDS             52..780
                     /locus_tag="PC523_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            908..2173
                     /gene="gluD"
                     /locus_tag="PC523_002141"
     CDS             908..2173
                     /gene="gluD"
                     /locus_tag="PC523_002141"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HDF2475661.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            2674..3813
                     /locus_tag="PC523_002142"
     CDS             2674..3813
                     /locus_tag="PC523_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HDF2475662.1"
                     /translation="MSNFILNGIFRTVIVSSLSILMILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKDININNINLEGFNNISTVISSILLYTWITSVVIY
                     LIYTIFKYIKLKNLISDLSYEVDDEEIINLYRSILKEFDITKDIKLKYSYEVETPAFF
                     NSCVLLPPHDYKLKELDWIFRHELMHFKSKDLYLKYVILFLKIVYWFNPFVYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTIINAMRKGTNTSNKFIAGLHKQSDIKKRVKN
                     MFNEKYKNGILMALTLCLLSSITFLKVNTTSVINFNVLPKGLEDAHVIGSITVTTIEF
                     TYADAPKNVREEHKRNCEELGVIPKDSDQIGVGVEYYNSLSKRSR"
     gene            4272..5294
                     /locus_tag="PC523_002143"
     CDS             4272..5294
                     /locus_tag="PC523_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2475663.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEENGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQINPSVNQNVDSTNTNQQNNANTSQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVRVTSINSAYYTARFV
                     TAKRIL"
     gene            5589..6509
                     /gene="pyrB"
                     /locus_tag="PC523_002144"
     CDS             5589..6509
                     /gene="pyrB"
                     /locus_tag="PC523_002144"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDF2475664.1"
                     /translation="MLESRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMKIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLENHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDG
                     DDRAVYFKQAKYGMYVRMALIIKLLGISED"
     gene            6548..7240
                     /locus_tag="PC523_002145"
     CDS             6548..7240
                     /locus_tag="PC523_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDF2475665.1"
                     /translation="MYKILENIYIGEDMYRMKVKGTFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDTIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEEEYKQVCNEVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNEGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            7233..8135
                     /locus_tag="PC523_002146"
     CDS             7233..8135
                     /locus_tag="PC523_002146"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDF2475666.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDNELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVEMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            8220..8804
                     /locus_tag="PC523_002147"
     CDS             8220..8804
                     /locus_tag="PC523_002147"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDF2475667.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            9021..9947
                     /locus_tag="PC523_002148"
     CDS             9021..9947
                     /locus_tag="PC523_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HDF2475668.1"
                     /translation="MKNQLNKSQKKVVFFGVGAVGATFAEQFLNSKYDFKILCNKERK
                     KRYLEDGFIINGKRYDFDYVTRDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYVLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKGISLTEDDVENSLHRILEHSKEGRTSMLQDVEAHRPTEVDMF
                     SKNICKLGKKYSIPTPINQTFFYMIKVIESRF"
     gene            complement(9998..10888)
                     /locus_tag="PC523_002149"
     CDS             complement(9998..10888)
                     /locus_tag="PC523_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2475669.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSQKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQDQKWIVREDGSGTRDYLDMFLSVKEIIPRSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            11076..12107
                     /locus_tag="PC523_002150"
     CDS             11076..12107
                     /locus_tag="PC523_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDF2475670.1"
                     /translation="MLKKKSILTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKILRVIFLVVVVLVFGHMKNQSNKEILEEEKDEIKKKKVTIPWYVIGFFVTCALF
                     SMNIIPKEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFIGIVQVVS
                     AVVLIKIFI"
     gene            12401..13276
                     /locus_tag="PC523_002151"
     CDS             12401..13276
                     /locus_tag="PC523_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine DNA glycosylase"
                     /protein_id="HDF2475671.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGKEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDKVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            13294..14784
                     /gene="cls"
                     /locus_tag="PC523_002152"
     CDS             13294..14784
                     /gene="cls"
                     /locus_tag="PC523_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDF2475672.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGIGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNIGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTEDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            14977..15261
                     /locus_tag="PC523_002153"
     CDS             14977..15261
                     /locus_tag="PC523_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDF2475673.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            15294..16922
                     /gene="groL"
                     /locus_tag="PC523_002154"
     CDS             15294..16922
                     /gene="groL"
                     /locus_tag="PC523_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDF2475674.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEERVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVIKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            17184..17924
                     /gene="larB"
                     /locus_tag="PC523_002155"
     CDS             17184..17924
                     /gene="larB"
                     /locus_tag="PC523_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HDF2475675.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYSHAEYEDLSK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLAAMINKL"
     gene            18008..18742
                     /gene="larC"
                     /locus_tag="PC523_002156"
     CDS             18008..18742
                     /gene="larC"
                     /locus_tag="PC523_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HDF2475676.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLKMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDESNLNENVKSKAKKIFMA
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATMEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDEFEFSPRQI
                     GYGIGHKKFEVPNMLRTVLGEKKKKN"
     gene            18772..19191
                     /locus_tag="PC523_002157"
     CDS             18772..19191
                     /locus_tag="PC523_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HDF2475677.1"
                     /translation="MSSEIYSYLYEKILGEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGELVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      19251..19352
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            19573..21108
                     /gene="guaA"
                     /locus_tag="PC523_002158"
     CDS             19573..21108
                     /gene="guaA"
                     /locus_tag="PC523_002158"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDF2475678.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIETVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            21454..21864
                     /locus_tag="PC523_002159"
     CDS             21454..21864
                     /locus_tag="PC523_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475679.1"
                     /translation="MGKINSYNKEMESILIFDIGGISVKNTFSEVKLFQVKPDRLDDF
                     EKLIVLVAEEQKQQTGCIDIKYLKRFYIFDNIKEQPRELTKIVKCVKYYSYWEFDTLE
                     NYSNATHWFFDTYSKEVFKLLIMPFDINCGNSIY"
     gene            22008..22289
                     /locus_tag="PC523_002160"
     CDS             22008..22289
                     /locus_tag="PC523_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475680.1"
                     /translation="MLFIVIYILILFTIYLVKKNEFIGYILARGMLKLCFIIGIMLSI
                     QTVFQLVNKIEIPVPALITKVLIFVVLAVFARYIERNLKGNFIYSNSDF"
     gene            complement(22328..22900)
                     /locus_tag="PC523_002161"
     CDS             complement(22328..22900)
                     /locus_tag="PC523_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2475681.1"
                     /translation="MINGDKISLIPATLDDRQKVYEWCFQSETTKSHSGLPNYPNIPI
                     ATYEEFCEEDYEEYFFTGAKPKDGRGFLIVSNTEPVGFISYSCFHLKQALAEIDIWMN
                     CEANCGKGFGVDAIISLGDFLNKTMDICELIIAPSRKNIRAVKAYKKAGFKPSTKPMS
                     DYLLDKYIPLYGTGDYGIDETVILVKQLDT"
     gene            23322..23564
                     /locus_tag="PC523_002162"
                     /pseudo
     CDS             23322..23564
                     /locus_tag="PC523_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
BASE COUNT         9346 a         2463 c         4321 g         7555 t
ORIGIN      
        1 gcaaaattct atgaagcagg aaccctgtga ctttagtcat gggaggttca ggatggaatt
       61 ttaatataca aaaacaaggt tttagctgga gtatcagcaa aacaagcaaa tactactata
      121 gaagcagtta aaagggctat tttttataat ctggagcata gacaagaagt tagagaaatg
      181 attaaaaaag aaaatataag aaaaattctt attattggaa catcaaaaaa gatggttaat
      241 caaattgtag atagattgtc aattggaagg gtttgtaagt ttataaatat agaagatata
      301 agtactaaat atgaaataga agtagcaaaa caatctagaa aagaaggaaa tcatgtaatt
      361 cctgttccag cagttgagat aaaaagtatg gcaagtggtc ttagtataaa ttctttgaag
      421 agacttttta ggaaaggaaa taataggaat atggtagttt tagaaaaaac aattataaga
      481 ccaaccttta gttatatagg aaagttttat attagtgctg atgtaattaa acagattatt
      541 gaatatgaag tatacagctt tggtagcatt gatagaataa ataaaatcaa tatagagaat
      601 tgcaataatt ttatgaatat ttttgtaagt ataaatatca atgatttaga aactataaaa
      661 aaaattgaag atatacagca gataataaaa aagtctattg agaaaatgac tttagttaat
      721 gtacaaaaaa tagatattaa cattcataaa attaaaaaaa taaacttcct ccaatgttaa
      781 aaaataaact gagaaaatga tatactaatt tgcaataaag tgtgctataa ttaaactgta
      841 aatttaggaa atggtttttc taaatggagt atacataatt ataacgtatt tataaggggg
      901 ctttcaaatg tcaggaaaag atgtaaatgt cttcgagatg gcgcaatctc aagtaaaaaa
      961 tgcatgtgat aaattaggta tggaaccagc agtttatgaa ttattaaaag aacctatgag
     1021 agttatagag gtttcaattc cagtaaaaat ggatgatggt tctataaaaa cttttaaagg
     1081 atttagatca caacataatg atgcagtagg gccaacaaaa ggtggaataa gattccatca
     1141 aaatgtttca agagacgaag taaaagcttt atctatatgg atgactttca aatgttctgt
     1201 aacaggtata ccatatggtg gaggtaaagg tggaataata gtagatccat caactttatc
     1261 tcaaggtgaa ttagaaagat taagtagagg atatatagat ggaatatata aattaatagg
     1321 tgagaaagtt gacgttcctg caccagacgt aaatactaat ggacaaataa tgtcttggat
     1381 ggttgatgag tacaataaat taactggaca aagttctata ggtgttataa ctggtaagcc
     1441 agttgaattt ggtggttctt taggaagaac agctgctact ggatttgggg ttgctgttac
     1501 tgcaagagaa gctgctgcta aattaggaat agatatgaaa aaagctaaaa tagctgttca
     1561 aggtatagga aacgttggtt cttatacagt tcttaactgt gaaaaacttg gtggtactgt
     1621 tgtagctatg gctgaatggt gcaaatcaga aggttcttat gctatataca atgaaaatgg
     1681 tttagatggt caagctatgt tagattatat gaaagaacat ggtaacctat taaacttccc
     1741 aggagctaag agaatatctt tagaagagtt ctgggcttca gatgttgata tagtaatacc
     1801 agctgcatta gaaaactcta taactaaaga agttgctgaa tctataaaag ctaaattagt
     1861 ttgtgaggct gctaatggac caactactcc agaggctgat gaagtatttg ctgaaagagg
     1921 aatcgttctt actccagata tattaactaa tgctggtgga gttacagttt cttacttcga
     1981 gtgggtacaa aacttatatg gatactactg gtcagaagaa gaagtagagc aaaaagaaga
     2041 aatagctatg gttaaagcat ttgaatctat ttggaaaatc aaagaagagt acaatgttac
     2101 aatgagagaa gctgcttaca tgcattcaat taagaaagtt gctgaagcta tgaaattaag
     2161 aggatggtac taggatttac tcttaataaa taataggata taattattta agatataaag
     2221 gggctgtaca tagtacagcc cttaaattat gtggctcagg taatgtgagc cattttttat
     2281 tttaatttat aaattatata actcaaaatt taaaacgcta taagtaagac gagtgataat
     2341 ttatggttaa tttaatttta agtaggcttt ttagaactat tctagtgaat agtttatgta
     2401 ttcttttaat attagcattt aagaaaagta tttttgttct attttaactt tgcttttata
     2461 tatttataac tgtcaaagtt atatggagtt aaattaattg atgatgttta atgcacagta
     2521 atcattacta agctataatt aactataaga taataaaaca tagttattta ttacagaaaa
     2581 tatatatttt aattaataaa tatgatatga aagtgttaag agataactaa aattaactat
     2641 aaaacactat taaataagat aggggaaaaa catatgtcta attttattct aaatgggatt
     2701 tttagaactg ttatagtgag tagtttaagt attcttatga tattaatatt caagaaaaat
     2761 gtgtttaaaa gatttagtaa aaaatttaac tattatatat ggatgatagt tgttattaaa
     2821 ttatttctac catttacata ttatactttt acaataaata tattaagaag caaaaaagac
     2881 attaatatta ataacataaa cttagaggga tttaataata tctcaactgt aattagtagt
     2941 attttactgt acacttggat tacatctgta gtaatatatt taatttatac tatattcaaa
     3001 tatataaaac ttaaaaattt aataagtgat ttatcttatg aagttgatga tgaagaaata
     3061 ataaatcttt atagaagtat attaaaagaa tttgatataa caaaagatat aaaactaaaa
     3121 tactcatatg aagttgaaac accagcattt tttaattcat gtgtgctttt gcctccacat
     3181 gattataaat tgaaagaatt agattggatt tttaggcatg agctaatgca ttttaagagc
     3241 aaagatcttt atttaaaata tgtaatttta tttttaaaaa tagtatattg gtttaatcca
     3301 tttgtatata taatggataa acatatagat ttagattgtg agctttattg tgatgaacgc
     3361 gttttaaaaa atagaaactc agaggaaaaa aaagattacg ctttgacgat tataaatgcc
     3421 atgagaaaag gaacaaatac ttcaaataaa tttatagctg gtctacataa gcaatctgat
     3481 attaaaaaga gagtaaagaa tatgtttaat gaaaaatata agaatggtat attgatggca
     3541 ttaaccttat gtctgttatc ttctattact tttttgaaag ttaatacaac tagtgttatt
     3601 aattttaatg tattacctaa aggcttggaa gatgcacatg ttattggtag tataactgtt
     3661 acgacgatag aatttacata tgcagatgct cctaaaaatg tgagagaaga acataaaaga
     3721 aattgtgaag aactaggagt gattcctaaa gactctgacc aaataggtgt tggtgtagag
     3781 tattataaca gtttatcaaa acgatcaaga tagtaatatt ttaatagatt gtaatttata
     3841 atagttataa agttttaaac agttatttat taagaaaaaa tatattttta taaatgtata
     3901 tacaaaatca caaaatgact aactttatag ttattccctc aaaaaacata tgccacatag
     3961 aaacgcataa aaaaacacat aaaacaatat ttatgcgtac tttagcctct aaaggtacaa
     4021 aattctttac ttttacagac tcgtattata taattaagta aataatgtat atttaggtga
     4081 catattggat aaactaattt atttatttta ttaatattat aatactataa aagttatata
     4141 aagtggttta caaaaaagtt acagatagag cttttattaa tgtattgtgt attagtattt
     4201 tatccttagg tacacttcac ctacaaaata gaatcataag ataattatgg gaaaatttta
     4261 ggaggtttat cgtgaagaaa aagatattaa taccagtatt tgcatcagtt atggcattat
     4321 cagtaagttc aattgtaaat gcagatgaag taaatgattc atctcaaaat aaggatgata
     4381 aaacaaatac tgaattaaat ttaggtgaat ataaagaagt aaaatataaa gttgcaaaaa
     4441 taaaagatgg agtagctata aagataagag aagaaggtca agttcaaaat atagcttatt
     4501 ctggagatga gtttactgtt ctaggaactc aaggagaatg ggttaaggtt aaagttgaag
     4561 atggcgaagg ttggttagct actagatatg tagatatatc tgaaggtgta ggatatacta
     4621 acgctgataa agtaaattta agaaaagata agagtgaaag ttcagaagta attgaagagc
     4681 tagaaaaagg aagctcttta ttggtcttgg aagaaaatgg agattggtta aaagttaaag
     4741 atggggaaac cgaaggatat gtaaaatcat cttatatatc tgataaagct cctgtaatag
     4801 aagagccaca aataaaccca agtgttaatc aaaatgtaga ttctacaaat actaatcaac
     4861 aaaacaatgc aaatactagt caacaaaaca ataattcaaa tgtgccaaca gctaactcta
     4921 atgcagttca agctgtactt aatttggcat attctaagca aggttgccca tatgtttggg
     4981 gagcagaagg accaaacact tttgactgtt ctggatttac acaatatgtg tatagaaatg
     5041 cagtaggaaa gaatatacca agaacatcta aagcacagtc taaatatgga caaactgtaa
     5101 gtaaagcaaa tttacaacca ggagacttag tattcttcac aactaatggt tctggttctg
     5161 ttagtcatgt tggaatatat gtaggtgggg gtaatatgat acattcacca tcaacaggaa
     5221 aaacagttcg tgtaactagt ataaattcag catactatac agcaagattt gttacagcaa
     5281 aaagaatatt ataatcagtt tgtaatatga caaattgata aatacataaa tatatataaa
     5341 cgctctatat tatatttaat ttaatcatgg aaataaaaaa tgattatatt ttaaatgtac
     5401 tatagagcgt ttttgtatca ctttaaatct aatcaggatt cagggtgata ttactttaaa
     5461 taggagcatt ttcatatgat gattatttgc gcaatccctt gacaggatag ggcaaaggtg
     5521 gtaagctact aacagtacaa aaatactttt aattttagtt cagagaggct aaaaaaggag
     5581 gacgaaagat gttagaatca agaaatttaa tccaaccaga agacttttca atagaagaaa
     5641 tagatgaaat attagagtta gctcaaaaga taattgacaa tccgtcaaaa tattctcgta
     5701 tatgtgaagg aaaactatta gctacattat tttatgaacc atcaacaaga actagattaa
     5761 gctttgaatc tgctatgaac agactaggag gtcgtgtagt aggcttttca gagccaaatt
     5821 catcttctgc atcaaaaggt gaaaccctag gtgatacaat gaagatagta tctggttatg
     5881 ttgatataat agctatgaga catcctcaat caggtgcagc atcagaagca gctagatata
     5941 cagaagtgcc atttataaat gcaggagatg gcaaaaatca gcatccaact caaacactta
     6001 cagacttact tactataaaa tcattaaaag gaactttaga aaaccataca ataggattat
     6061 gtggtgattt aaaatatgga agaactgtgc attctttggt taaggctatg gcaagatata
     6121 aaaatacaaa gtttgtattt atagcaccag aagagttaaa aatgccagat tatataaaag
     6181 aagctataaa gggacatgca tattatgaaa ctaataattt ggatgatgtt ataggaagct
     6241 tggatgtttt atatatgact agagttcaac aagaaagatt tgaagataaa tcagaatatg
     6301 aaagacttaa aaactattat attttaaata aagccaaatt agaaaaagca tctaaagata
     6361 tgttagtaat gcatccatta ccaagagtaa atgaaataga catagacgta gatggtgatg
     6421 atagagctgt atattttaaa caagctaaat atggaatgta tgttagaatg gctttaataa
     6481 taaaactttt aggtataagt gaagactaat agttatcata taatagtaaa ataaaggaga
     6541 ttttgggatg tataaaattc tggaaaatat atatataggc gaagatatgt atagaatgaa
     6601 agtaaaaggg acttttgaag gtaaaatggg acaattctat atgctaaggg catgggacac
     6661 ttatccagta ctttcaagac ctataagtat acatgatata gatgaagaag ggataacttt
     6721 tctttataaa gtagtaggag aaggaactca aattttgagt aacctaaaag taaatgatac
     6781 tataaaatta gaaggccctt atggtaatgg atatgctaag gttgatggaa aggtagctct
     6841 agtaggagga ggaatcggtg tagctccact atatttggtt gctaaaaata taaaaaattg
     6901 tgacgcatat ctagggttta gagaagatgt tatattagaa gaggaatata agcaagtatg
     6961 taacgaagtc tatacaacag taggaaatac ttttgtaaca gatataattg atgtggagaa
     7021 atatgattat atattaacat gtggtccaac tccaatgatg gaaaaattag ttaagatggt
     7081 agaaggtact aaaacgagaa ttatggtatc tcttgaaaat catatggctt gtggagtagg
     7141 tgcttgttta gtgtgtacat gtaaaactaa tgaaggaaat aaaaagactt gtaaagatgg
     7201 tccagtgttc tggggagagg acgtgatttt taatgggtaa tttaagtgtg aaatttggaa
     7261 gtgtagaatt taaaaatcca gtaataatgg cgtctggaac ttttggattt ggaaaagaat
     7321 ataatgaaat ttatgatata caaaaacttg gtggtataag tagcaaaggt cttactttaa
     7381 ataaaaaacc tggtaacaat ggtatgagag ttcatgagac accgtctggt atgatgaata
     7441 gtgttggtct tgaaaaccct ggagttcaag gttttataga caatgaatta ccatttttca
     7501 gtaaactaga tttagtaaga atagctaatg ttggaggtgg gacattagaa gactacttat
     7561 taggggttca aatgttaaat gataaaccta tagacattat tgaattaaat atatcttgcc
     7621 caaatgtcaa agctggtgga atggcttttg gtataaaaaa tgaagtggca agagaagttg
     7681 ttcgtgaggt aagaaacata acgaaactgc ctttagtaat aaaattatca ccaaatgcag
     7741 aagacattgt agaaatggca aaagtttgtg aagaagaagg tgctgatgga gtttcgttag
     7801 ttaatacatt taaagcaatg gcaattgata taaaaaatag aagaccagta tttgaaaatg
     7861 tatatgctgg gttatctgga ccagctataa aacctatagc tttaagaatg gtacatgaag
     7921 tgtgtaaaaa tgtaaatata cctgtaatgg gaatgggcgg aataactaaa gcaacagatg
     7981 ctatagaatt tataatggca ggagccactt gtattcaagt tggaactgca aactttatga
     8041 atcctagaat cggtattgaa ataatagatg gaattaatga atttatggat agagaaggaa
     8101 taaaaagttt agacgaaata agaggaatta tataaggtag aataaatagt attttaaaga
     8161 taaaaaatat agattaaata aacaataatt ttataacatt aacatggagg aaaaataata
     8221 tgagtaaagt agatgtagta gatatattaa agaaaagtga tgcattgttg gagggacatt
     8281 ttttattatc ttcaggaaaa cacagtaata gatatgtaca atgtgcaaaa gtattaagat
     8341 tcccagaata tgctgctgag gtattaagta cagttgttga acaaataaaa gacttagata
     8401 tagacttagt agtagggcca gctatgggtg gagtaatagt ttcttatgaa ttgggaagac
     8461 aattaggaaa agaagctgta tttactgaga gaaaagacaa tacaatggag ttaagaagag
     8521 ggtttgaagt taaaaaagga gcaaagataa taattgctga agatgttgta actactggta
     8581 aatcaactat ggagacaaag agagtattag aagccttagg tggagaagtt gtaggtgttg
     8641 catgtatagc agatagaact aatcatgata taggtatgcc tatatatagt gctataaaac
     8701 ttgatattca agtttatgaa tctgatgagt gtcctttatg taaggaagga aaattaccag
     8761 ttgttaagcc tggaagtaga gagttcaagg aattaggaat gtaataaaaa cttaattatt
     8821 tatagtagta ttaaaaaaaa gtgaattttt cagaataaaa atttggaata taaaatataa
     8881 attaaaaata aagagcagtt agccttataa atgaaggtta gctgtttttt tgttaaaaat
     8941 atatttacaa aataagagat agtagcagta caatataagt ataaaataaa aaccattgta
     9001 tgcattaggg aggggtaagt atgaaaaatc aattaaataa aagtcagaaa aaggttgtat
     9061 tttttggagt aggagctgta ggagctactt ttgcagaaca atttttaaac tctaaatacg
     9121 attttaagat tctttgtaat aaggaaagaa aaaaacgata tttagaagat ggatttataa
     9181 taaatggaaa aaggtatgat tttgattatg taactagaga tgaatataaa caagaagctg
     9241 actttataat tataggttta aaatataata atttgaaaga aaatataaaa gaattagatg
     9301 gattagttgg aaaaaataca gtcataatgt ctctgctaaa tggagttgat agcgaagaga
     9361 taataggtga aagatttgga attgaaaaaa tggtatattc atatgttacc aatatagatg
     9421 caaagaaaat caataataat attatacaca ctactaatgg gataattgta tttggtaaca
     9481 aagataacag tgaagataga aaaactaata taataactga ggtttttgat gatgtaaata
     9541 tagaatatgt tttatcaaaa gatattcaac gagatatgtg gtggaagtac atggttaata
     9601 ttggtgtaaa tcaaacttca gctatacttg gtgcacctta tggagttttt cagagttctg
     9661 aacatttaag agaattagca aaatctgcaa tgagggaagt tgttgctata gcccaagcaa
     9721 aaggtatatc tcttacagaa gatgatgtgg aaaattcatt acatagaata ctagaacatt
     9781 caaaagaagg aagaacatca atgcttcaag atgtagaagc tcatagacct acagaagtag
     9841 atatgttttc taagaatatt tgcaaacttg ggaaaaaata tagcatacct actcctataa
     9901 atcagacctt tttttacatg ataaaagtaa ttgaaagtag attttaaggg tttttgtaag
     9961 aaactttgat aattattgat tttaaatttt attaatgtta tatactattt aagttactaa
    10021 atatttttaa ctcttcaatg aaaatagttg ctgcttttga taaagttata tcttttggaa
    10081 atatatatga gaaatgtctg ttgtagctgc ttccaagctc tatgacagac aactcattgt
    10141 ttaaaacagg taagcttgtc actagattag agactattgt aattcctaaa ttatttctaa
    10201 cagattcttt gacagcataa ttacttccaa aaaccatcat actccttgga ataatttctt
    10261 taacacttaa gaacatatct aaataatctc tagttccaga cccatcttct ctgactatcc
    10321 atttttggtc ttgaagttta tcaaaactaa aatcttttaa caaatgactt ttgtatggaa
    10381 gtgctagaac catcttatct tcaaaaaaat attcttgaat aaatgaaggg gaagagcaag
    10441 taccttcaat aagaccaatg tctaaaatat aatccttaac atgagaagat acgattgacg
    10501 tattttttat aaaaacttct acatctatgt caggatactt ttgagaaaat aacgctagaa
    10561 aatttggtag aatatactct ccaatagtta aacttgcgcc aatctttaag tgaccagtta
    10621 tagcatctga aacttggctt acatcatgat aagtaatatt taaaagattt aatatttctt
    10681 tagctctttt atacaatatt tgtccacttt ctgttataaa aatggttttt tgtttaatag
    10741 acctgtttat aagcacaact ttaaaataat tttctaaatt ttttatgtgt ttacttacac
    10801 ttggctgtga taggtttaag tattccccag ctttagtaaa atttttgtat tcaacaacag
    10861 ctacaaatgt ttttaattct tcaaacaatt ttttgtctcc tttacctaat taaattttac
    10921 attttttata tactttctac agataattat actatatata ataaccatta aaaacattaa
    10981 tcattacgat attatatatt tattttactt atggttatta caattatata ctattgctat
    11041 gaagaaaact atattaatta taaggtaggt atattatgtt aaagaaaaaa agcattttga
    11101 cattaaaaac tataagggaa atattaccag ggttgtttgt atcagtatta gtaggttata
    11161 ttagtatatt tatttctatt ttaattccta aggtaggagc agcatcaatt tctatatttt
    11221 taggtatgtt tgtaggaaac ctatttttaa atcaaaaagt gtttcaaaaa ggttataaat
    11281 tttcagagac agatttgtta tcatattcaa tagtactatt aggagcaaca ctcagtgtat
    11341 ctaccttaat agacttaaaa gtttcaggaa ttttatttat aatattgcaa atgacaataa
    11401 caataatagc tgcactttat ataggtaaaa aactaggatt tgaagaaaac tttagatttc
    11461 taatggcaag tggaaatgca gtatgtggtt cttctgctat aggagcaaca gttccagtgg
    11521 ttaatgcgac agataaagaa aagggaattg cagtaactat tgtaaatgtt actggtatat
    11581 tccttatgtt tttacttcca ataatatctc aatttttata tagccatgaa cttgtaaaaa
    11641 cgtcagcaat gataggtggg actttgcaat caattggtca ggttgtagca agtggagcaa
    11701 ttgttggaga aaatgtaaaa gatttggcta ctatttttaa gatattaaga gtaatatttc
    11761 tagttgttgt tgtattagta tttggacata tgaaaaatca gtctaataag gaaatccttg
    11821 aagaagaaaa ggatgagatc aaaaagaaaa aagtaactat accatggtat gtaattggat
    11881 tctttgtaac ttgtgcactt ttttcaatga acattatacc aaaagaagta tctgtgttgt
    11941 gcaaagaaat aagtaacaaa ctagaaataa tagctttagc agcaattggt ttaaaggtta
    12001 atgtaaaaga tttagtgaaa caaggaaaag aagtttcttt atatggttta tttataggta
    12061 tagtacaagt tgtatcagct gtagttttaa taaaaatatt tatatagcac aaaaatagta
    12121 tttgtaatat caatttcatt aattaaaata atatgatact aataatttat aatataaata
    12181 aaaaatgaat ttcgataagt ttaagtgggt ataaacttat cgaaattcat tttttattta
    12241 aggtaaaaat ttaaagtaaa atattatttt acataataat aaccttataa attttgaaat
    12301 tacttatctt tgtgttaaaa taagtaaaat gcacatatta aagctaagtt tttaagtaaa
    12361 taaacaaaat gaaaatataa atattaagga gatttagtat atgaatgttt atgaaaaagg
    12421 taatggagtt atactagaag gagtaacaga ttttgaccca gtacatatat ttgagtgtgg
    12481 tcaatgtttt agatggcaca aacaagaaga tggctcatac actggtgttg caaagggaag
    12541 aatattaaat gtcaaaaaag agaatgataa aatatattta aataatacga atttaaagga
    12601 gtttaatagt atctggtata attactttga tttaggaact gattatacag aaataaagaa
    12661 taagctaaaa aatatggatg aatatttaaa taaggctact gaatttggat ggggaataag
    12721 aatattaaga caagatggat gggaaatgct catatcattt ataatttcct ctaataatag
    12781 gattcctatg atacaaagag ctatagaaaa tttatcaaga aaatttggta agtatatagg
    12841 tgaatatgaa ggtaaagaat actatgcatt cccaacacca gaagagttaa ataaggcctc
    12901 acaagaggaa ataagagcat gtcaaacagg ttttagagat aagtacataa aaagtactac
    12961 acaggcagtt atagagaata atgataaggt atccgaatat acaaatttaa gtacagaaga
    13021 ttgtagaaaa gagcttttaa aatttaatgg agtaggccca aaagtatgtg actgtattgc
    13081 attatttggt atgcaaaaat atgattcttt tccagttgat gtttgggtta agagagttat
    13141 gcaagaattt tatatagatg aagacatgag tttacctaaa atgagaactt atggaatcga
    13201 taaatttaaa gaaatgtctg gatttgctca acagtactta ttttactatg caagagaact
    13261 cggtatagga aaataaatta agggggagaa attatgggag taataggaac aatatttctg
    13321 ttctatttaa ttatatcata tctggctgga gccataattt ctgtgattat attacttgaa
    13381 aatagagatc ctgcaaaaac aatgtcttgg ctattaatgt ttattatatt tccaggtata
    13441 ggtttaatga tatatgctat ttcaggtaga aatattagaa aaagaaaatt atttaaaact
    13501 caaaaactag ctaataatat aaaagaaaag aaactttttg atacattaga aaagataaca
    13561 gaaatagttg aattagaaaa agaatctata aaacaaaata agcttttaag agacgaagaa
    13621 gatggtagtt atagaaaaag ggtaataaat atgctattaa agactggtat gtttcccttt
    13681 actaaaaata ataaagtgga tgtgtttgta gatggaaatg aaaaatttaa aagacttata
    13741 gaagatataa gagaagctaa agaccatatt catttagagt attttatcat caaagatagt
    13801 gaaataggta gggtgttaaa agaggaactt ataaaaaaag ctaaagaagg tattaagata
    13861 agaattttat atgatgatgt aggatgctgg agattttggt ttaatagaaa gtttttccga
    13921 gaaatgaggg aagtaggtat agaaattgca gcatttttac ctacaaaatt tcctataata
    13981 ggaggaaaac taaactatag aaatcacaga aaaattgttg ttattgatgg aataattgga
    14041 tatacaggtg gaataaatat aggagatgaa tatttaggaa aaaatgataa gtttggatat
    14101 tggagagata ctcatattag gattaagggt atttctgtat acatgcttca aatgacattt
    14161 ctgattgatt ggtattatac gacaaaagaa gtattagtga ctaaaaatta ttttccaagt
    14221 gtagggaata ttggtgaaag tatgatacag gttgttgcca gtggtccaga tagtgactgg
    14281 gaagatattc attatgctta tttttctgca atatgtcaag ctagaaaaaa tgtttatata
    14341 gaaactccat atttcattcc agatgaaagt ctattaaaag ctataaaaag tgctgctctt
    14401 agtggagtag atgtgagaat aatatttcct aaaattgcag accacaagat agtaaacata
    14461 gcatcttatt cgtattttga agaaatatta agagcaggtg gtaaagttta tttatataat
    14521 aaaggattta tacactctaa agtcgtaata atagatgata aaattgcttc tgcaggtaca
    14581 gccaatatgg acttaagaag ttttatgctt aactttgaag taaatgcatt tatctatgat
    14641 gaagaagtta ttagagtaat gacagaggat ttctttgagg atttaagcca ctgtgaagaa
    14701 cttaatttgg aggtatttaa gaatagaaat atcatacaaa aaatcaaaga atctgtagca
    14761 agattatttt caccaatatt ataaaaaatt attaaaaaag gtattgaaaa ttatgtgtga
    14821 ttggtatatt ataataattg ttagcactct attagataga gtgataataa ataaaaagta
    14881 tatatataaa tctacatata aaagtagtat taatttaaaa ataactaaaa acataggcga
    14941 actagtctat acttaatatt ttaggaggcg taaataatga aaataagacc attagctgac
    15001 agagtagtaa ttaaaaaagt agaagcagaa gaaaaaactg caagtggaat agttttacca
    15061 ggagcagcta aagagcaacc tcaaatagct gaagttgtag aagttggacc aggtggaata
    15121 gttgaaggga aagaaataaa aatggaattg acagtaggag ataaggttat attccaaaaa
    15181 tattctggaa cagaagttaa gatagaagga caagaataca caatactaag acagagtgat
    15241 gtattagctg ttattgaata aatatagaat aaatttatta ggaggagttt aaaatggcta
    15301 aagaaattaa attttcagag gaaacaagaa gagctttaga agctggtgta aataaattag
    15361 cagatacagt aaaagtaaca ttaggaccta aaggaagaaa tgttatatta gataaaaaat
    15421 ttggttctcc acttataact aatgatggag taactatagc aaaagagata gagttagaag
    15481 atagatttga aaatatgggt gctcaattag ttaaagaagt agcaactaaa actaacgatg
    15541 tagctggaga tggtactaca actgctacag ttttagcaca agctataata agagaaggtt
    15601 taaagaatgt aacagcaggg gctaacccaa tacttttaag aaaaggaata caaaaggcag
    15661 taacagtagc agtagaagaa ttaaaaaatc aatcaagaat agtagaaaca caagaggcta
    15721 tatctcaagt tgcttctata tctgctggag atgaagaggt tggaaaatta atagcagaag
    15781 ctatggaaat agtaggtaaa gatggagtta taactgttga agagtctcaa actatgaata
    15841 ctgaattaga tgctgttgaa ggtatgcaat ttgatagagg atttgtttct gcatatatgg
    15901 ttacagatgt agataaaatg gaagcagttt taaatgatcc atatatatta attactgata
    15961 aaaaaatatc taacatacaa gagttattac cagttcttga gcaaatagtt caacaaggta
    16021 aaaagttatt aataatagct gaagatgtag aaggtgaagc gttatctaca ttagtagtaa
    16081 ataaattaag aggaacattt gatgtagtag cagttaaagc tccaggattt ggggatagaa
    16141 gaaaagaaat gcttcaagat atagcaatac ttacaggtgc tcaagtaata tcagaagaat
    16201 taggctatga tttaaaagaa gctgatttat ctatgttagg tagagcttca tctgttaagg
    16261 taactaagga aagtactaca atagtagatg gttctggaga taaaaaagct atagaagaga
    16321 gagtaactca aattaaacat caagtagagc aaactacttc agattttgat agagaaaaat
    16381 taatggaaag attagctaaa cttgctggag gagtagctgt aataaaagtt ggagctgcta
    16441 cagaagtaga gttaaaagaa agaaagttaa gaatagaaga tgctcttaat gcaactagag
    16501 cagctgtaga agaaggaata gtagctggtg gaggaactgc ttttgttagt gttataccag
    16561 caataggaac gctaattgag agtttagaag gagaagttaa actaggtgct caaatagtta
    16621 aaaaagcgtt agaagaacca ttaagacaaa tagctataaa tgctggtctt gaaggtgctg
    16681 taattgtaca aaatgttgtt aattctgaag ctgaaactgg atttgacgca ttaaatgaaa
    16741 aatatgtaaa tatgatagaa gctggtatag ttgacccaac taaagttagt agaagtgctt
    16801 tacaaaatgc tgcatcaata gcaagtactt tcttaactac tgaagcggct gttgctgacc
    16861 ttcctgaaaa agaagacgca ggaatgccag gaatgggtgg cggaatgcca ggtatgatgt
    16921 aatttaaaaa atttaaaata taaattttaa tataaagaac tatttcgact tagattttaa
    16981 actctaagtt gaaatagttc ttttgtatat atctagtttt tgtatattta taaattcaat
    17041 acacatattt agataatcag tatctaggtt gtgataatat cttagagtag tattgttatt
    17101 ttttaggtta tgattttaga tatatattaa ttaaggtata attgttgata gagactaaat
    17161 caaatttaaa aggatgcgat aatatggact taagaaagtt gcttgaagaa gttaaaaatg
    17221 aaaatattga tatagatttt gcgttagaaa aattaaaaga tttaccttat gaagaccttg
    17281 gatatgcaaa tatagaccat catagagaat taagaaatgg ttacccagaa gttatttatt
    17341 gtgaaggtaa gagtgatgag catattttag ggattattga taaaatgaat caaaaaagtt
    17401 ctaatatatt ggggacaagg tgtagaaaag aaacttttct aaaaattaaa gaaatctata
    17461 gtcatgctga gtacgaagat ttgtctaaaa ttcttaagat tcaaaatcat gatatagaga
    17521 atataggaaa aggtaaaatt gtgattgcta cagggggaac ttctgatata ccagtagcag
    17581 acgaagccta tcatacagct aagtttttag ggaatgatgt tgatagaata tatgatgttg
    17641 gagtggcagg tatacatagg cttttaaata aaagacataa aatagatagt gctagagtaa
    17701 tagtagctgt tgcaggtatg gaaggtgctc ttgcaagtgt aattggtgga ctagttgatg
    17761 ttccagtaat agctgttcca acatctgttg gatatggagc aaattttgga ggattagcag
    17821 ccttactagc tatgttaaat agctgtgcat ctggaatatc tgttgtaaat attgataatg
    17881 gatttggagc aggatattta gcagcaatga taaataaact ataaatacat tgatataaat
    17941 taaacaaaac tatataataa aatttaattt ctattatatt tttactatta aaattgagga
    18001 gacatatatg agtgaaaaaa tactttattt tgatataata agcggtatat caggggatat
    18061 gacccttgca agtttattaa atttaggtgt gcctaaggaa atttttttgg aggaaattca
    18121 taaactaaaa atgagcgatg aatttaatat aaacatcagc tctaaaactg aaaatggaat
    18181 agtaggtaca aaagtagaag taattacaaa agaaaaacat acacatagaa atttagtgga
    18241 tatatttgag gttatagatg aaagtaatct aaacgaaaat gtgaaatcta aagcaaaaaa
    18301 aatatttatg gcaattggag aagcagaagc aaaagttcat ggaactacaa tagacaagat
    18361 acattttcat gaggtagggg ctatagattc aattgtagat atagtaggag cttctatctt
    18421 agttgattta ctttgtgtag ataaagtttg tgctagtaca gtgtctgttg gctcaggatt
    18481 tgtaaaatgt gaacatggaa ttatacctat acctgcacct gcaactatgg aaattttaaa
    18541 aggtgtaccg attaaattaa ataatgtaaa tggagaatgt acaactccaa caggagctgc
    18601 aatcataaaa gtcttgtgtg atgagtttgt agacgagttt gaattttctc caagacaaat
    18661 tggatatggt ataggtcata agaagtttga ggtaccaaat atgctcagaa ctgtattagg
    18721 tgaaaaaaaa aagaagaatt aatttatgaa attagtgcaa acattgatga tatgtcttca
    18781 gaaatctatt cttatttgta tgaaaagata ttaggtgaaa aagcattaga tatttataca
    18841 gagagcattt atatgaagaa gaataggcca gcaacaaaag tatcaatttt atgcaatgaa
    18901 tcagatttag aaaaatttat taaaatatta cttttagaaa ctagtacttt tggagtgaga
    18961 tataatgcat ataatagaga aattctagag agaaaatttg tgaaattaga tacaaaatat
    19021 ggagtggtta cagtaaaatt agggtattat aaaggagaac tagtcaaggc gactccagaa
    19081 tataatgaat gtaaaactat agcaaaaaat gaagatattc ctataaaaaa agtgtttgct
    19141 gatataaatt ttagaataaa agaagaattt agtgaaaact tattgacata agtaataaat
    19201 tttgataaat taatattcag ccttattaaa taaaagtaaa aagaaaaaaa tatataaaat
    19261 ataaactcac tcgtatatgc tcagaaatat ggtctgagag tctctaccaa gataccgtaa
    19321 atatcttgac tatgagtgaa attattatac caaaagacct agttaagtaa atatgtaggc
    19381 tgagttgtac tccctatgtg ttggctataa gataaaggta ttttggaagc ctgtatagta
    19441 atttcacttt tgctagaaat tatggaagtt actatacagg ttttttgtgt aaaaagattt
    19501 aatataattt agactgtatt gtgttaaaat aaatatataa aaactatatt atacaaggag
    19561 agatgtgaat ttatgaaaca tgaattagta cttgtaatag attttggagg gcaatataat
    19621 cagttgatag ctagaagagt tagagaaaac aacgtatatt gtgaaatact tccatgtact
    19681 gcaagcatag aaagaataaa agaaaaaaat cctaaaggga taatatttac aggtggtcca
    19741 aatagcgctt atttagaaga ttctccaaca atatcaaaag aaatatttga actaggagta
    19801 ccaatacttg gaatatgcta tggtattcaa ataatgtctc atgtattggg tggagttgta
    19861 agaaaaggca ataaacaaga aaaagaatat ggaaaaacag ctataactta tggaaaatca
    19921 tcattatttg aaggaataac tacaaatagt gtatggatga gtcatacaga tttaatagaa
    19981 acagttccag aaggatttac tatagttgca aataccaatg attgtccagt agcagctatg
    20041 gaaaatgtag aacgaaacct atatggagtt caattccatc cagaggtaga acactgtttg
    20101 gaaggggaca agatacttac aaacttctta tataatattt gtaaagtaaa aggtgactgg
    20161 actacagatt cgtttataga agataaaata aaagaattaa aagaaaaaat aggcgataaa
    20221 aaagctttat gtgctttaag tggaggagtt gattcttctg ttgctgcagt tcttattcat
    20281 aaagcgattg gggacaactt aacttgtata tttgttgacc atggtttact tagaaaaaat
    20341 gaaggaaatg atgtagaaag aatatttaga gagaaatttg atataaattt aataagagtt
    20401 aatgcagaag atagattctt atctaaatta aaaggagttt ctgaaccaga agctaagaga
    20461 aaaataatag gtgaagaatt tataagagta tttgaagagg aatcaaataa attaggaaaa
    20521 atggatttct tagtacaagg aacaatctat ccagatgtta tagaaagtgg acatggaaat
    20581 gctgctacta taaagtctca tcataatgtt ggtggaatcc cagaagatgt agacttccaa
    20641 gaaattgtag aaccattaag agagttattt aaggatgaag ttagaaagat aggattagag
    20701 cttggtatag aagaaggatt aatatttaga catcctttcc caggaccagg tctagggata
    20761 agagttatag gtgatgtaac taaagaaaaa tgtgatatat taagagaagc tgatgctgtt
    20821 tatatggatg agcttagaaa agcaggttta tatagagaaa tatggcaagc ttttgcaaca
    20881 ttaccagatg ttaagactgt tggagttatg ggtgatgaaa gaacatatgc ttatctagtt
    20941 ggtttaagag cggttacttc ttctgatgga atgacttctg actggtataa gatgccttat
    21001 gatgtattag aaagaatttc taatagaata ataaatgaag ttgatggagt aaatagagta
    21061 gtttatgata taacttctaa accacctgga actattgaat gggaataaaa tatagaatga
    21121 taaaaatccg tatgaacaca ggatttgctc tatcttctta tcctatgcta tactagcttg
    21181 atattattca tatttgcaag tatcttgtag aataataagt aaaaagagca aaaatgctct
    21241 gctgaacaat aaattcagca gagtattttt ttattttatt ttgccgtgct cttgagctcc
    21301 aagcattaga catagatgat tttgttagat tttggtttta ttgtatcagc tctgaaaagt
    21361 gaattgatac aataaaaaga ggcatttatt gattattgat ggagatatat gttacgttac
    21421 ttttgttgga aatttgagag atagcgtggt ataatgggta aaataaattc atataacaaa
    21481 gagatggaga gtatacttat atttgatata ggaggtatat ctgtgaaaaa tactttttct
    21541 gaggttaaat tgtttcaagt taagccagac aggctagatg attttgaaaa attgatagtt
    21601 cttgtagctg aagaacaaaa acagcaaaca ggttgtattg acattaaata tttaaagagg
    21661 ttctatattt ttgacaatat aaaggaacag cctagggaac tgactaaaat tgttaaatgt
    21721 gtaaaatatt attcttactg ggagtttgat actttagaaa actattcaaa tgcaacacat
    21781 tggtttttcg atacatattc aaaggaagta ttcaagcttt taattatgcc ttttgacata
    21841 aactgtggaa attcaattta ttgaattgaa tagcaaatga gtagtatcac ctttggataa
    21901 tggaaagtca aggtattttc ttatggattt tgtggagtta agacactcga agttatgtac
    21961 aggattcttt ttttaaaaaa cctattttta ttggtttaaa tttgacaatg ctatttattg
    22021 ttatctatat attgattttg tttacaatat atttagtcaa gaaaaatgaa tttatagggt
    22081 atattttagc tcgaggaatg ttaaagttgt gttttattat aggtattatg ctttcaattc
    22141 aaacggtatt tcaacttgtg aataagattg aaataccagt tcctgcttta attactaaag
    22201 tgttaatttt tgttgttttg gcggtatttg ctagatatat tgaaagaaat ctaaaaggaa
    22261 atttcattta tagcaacagt gatttttaga tgtagtaatg aatgttgatg ttaaaaacaa
    22321 cctctgacta agtatccaac tgttttacta aaataacagt ttcatctatt ccataatctc
    22381 cagtcccata gagaggtatg tatttatcca ataaataatc actcattggt ttagtagatg
    22441 gcttaaatcc agctttttta tatgccttga cagcccttat attttttctt gatggtgcaa
    22501 taattagttc gcaaatatcc attgttttat ttaaaaaatc tccaagtgaa ataatggcat
    22561 ctactccaaa accttttcca caattagcct cacagttcat ccaaatgtca atttctgcaa
    22621 gagcttgttt taaatgaaaa caagaatagc taataaatcc aactggttct gtattactta
    22681 ctatgagaaa acctctgcca tcttttggtt tagcaccagt gaaaaaatac tcctcataat
    22741 cctcttcaca aaattcttca taagttgcaa taggtatatt tggataatta ggtagcccag
    22801 aatgagattt tgtagtttca gactgaaaac accattcgta tactttttgt ctatcatcta
    22861 atgtggcagg aattaaactt attttatcac catttatcat aatttttctc tccagtcttc
    22921 acatagttta atatttactt acagtataag cttaaattga taaattctaa cttacatata
    22981 ttgtaacata tccaaaatac ataatcactg aatattgtta tgtcgtaata gatttatgta
    23041 atacagtgtt agaaaatatc acatatcaaa aacatcagaa ttttactaga aatagtatat
    23101 tttatttata tttaatatta ttcataattt tttatattcc cctaattaaa aactctttgg
    23161 tatacttata aataaactta atgatagaat aaaaaagctt tataactatt agatgcatag
    23221 aaaagcattt tatgtctatg aaatgtatat ctataaaaca tatagatgac ttttatatat
    23281 taatgtatta atattgatat tatagaagaa ttgagttgtc aataatacta aatcaaatat
    23341 ctatagaata tggagggatt atgaaaaata aaataattat aggaattact ttttcacttg
    23401 tattactaat gtctggatgt gtttcaaaac aaacagcaga aataaacgta ttagcaacta
    23461 ctgatttaca tggagttata ccttatgagt taacttctta tgtaaaagaa gaaagaaaaa
    23521 aagataaaaa tctaacttta gtagatgcag gagatttttt tgattaaaaa atagctacat
    23581 acactcgtaa ctttagttat gagttagtag ctaaaatagt cagcatatag ggaaacttat
    23641 atgtagagat aggataacac ctatccaata ctacttgaat tgcta
//
LOCUS       DAMJVM010000050        23594 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.051, whole genome shotgun sequence.
ACCESSION   DAMJVM010000050 DAMJVM010000000
VERSION     DAMJVM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23594
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.051"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            13..480
                     /locus_tag="PC523_002163"
     CDS             13..480
                     /locus_tag="PC523_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDF2475682.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALITRILYFSDYFVVILFLIFIINSTKYSPFILENVKRFKMMGCCLLVNTIIECVVG
                     YKSNTSLIMIFGDNSGGISPLMVISFIFALMCFVIAETFDKAIKIKKDNDLTI"
     gene            491..706
                     /locus_tag="PC523_002164"
     CDS             491..706
                     /locus_tag="PC523_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2475683.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDENE"
     gene            849..1022
                     /locus_tag="PC523_002165"
     CDS             849..1022
                     /locus_tag="PC523_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HDF2475684.1"
                     /translation="MKRKLSTYFKGYVLGLCIAFLLFSFILKKLMISNIIGIVIGTGI
                     AFLLICLFNKKRK"
     gene            complement(1931..2215)
                     /locus_tag="PC523_002166"
     CDS             complement(1931..2215)
                     /locus_tag="PC523_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475685.1"
                     /translation="MIVYNKNFYPNDIFSRLDFSKIKRQLKLIDNELSDFGNICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYGIVEEIEKLFKSQVGKFSISTYRN"
     repeat_region   2931..3819
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3135..3163
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            4117..4302
                     /locus_tag="PC523_002167"
     CDS             4117..4302
                     /locus_tag="PC523_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDF2475686.1"
                     /translation="MKIHNLRYTGASLLHLVGTNMKIVSERFCHTDLKLTMNIYSYVL
                     EEMYKKTSNNLSKILFR"
     gene            complement(4455..5327)
                     /locus_tag="PC523_002168"
     CDS             complement(4455..5327)
                     /locus_tag="PC523_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2475687.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     gene            complement(5721..6299)
                     /locus_tag="PC523_002169"
     CDS             complement(5721..6299)
                     /locus_tag="PC523_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2475688.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            complement(6356..7072)
                     /locus_tag="PC523_002170"
     CDS             complement(6356..7072)
                     /locus_tag="PC523_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDF2475689.1"
                     /translation="MRINKYIASCGIASRRKAEEIILEGRIKVNGSIVKELFFNVDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVKERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISGDEIRKFETGLKIEDYTT
                     APAKIKVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            7263..8348
                     /locus_tag="PC523_002171"
     CDS             7263..8348
                     /locus_tag="PC523_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2475690.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEVKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYINSFIEKNMSNIDFLKTLIPHIQHSLDNVLKEASNFIGKISKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTTRNKADSIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            complement(8502..9176)
                     /locus_tag="PC523_002172"
     CDS             complement(8502..9176)
                     /locus_tag="PC523_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475691.1"
                     /translation="MTRRDLMRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLE
                     KHNYKKAMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEK
                     IKNGSSVIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYS
                     GSSKNNKSSSKNNKKSDSDKSDNNNNSSQNQEKPNSSNPTPAPTPQDTSGGSDSSGNS
                     QNSNNQ"
     gene            complement(9373..10665)
                     /gene="lysA"
                     /locus_tag="PC523_002173"
     CDS             complement(9373..10665)
                     /gene="lysA"
                     /locus_tag="PC523_002173"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDF2475692.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIKYFRAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSDYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIHLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHKAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEV"
     gene            complement(10683..11876)
                     /locus_tag="PC523_002174"
     CDS             complement(10683..11876)
                     /locus_tag="PC523_002174"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDF2475693.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            complement(12237..12854)
                     /locus_tag="PC523_002175"
     CDS             complement(12237..12854)
                     /locus_tag="PC523_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475694.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            complement(13024..14724)
                     /locus_tag="PC523_002176"
     CDS             complement(13024..14724)
                     /locus_tag="PC523_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475695.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVFHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQNFITDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNELKDEVIEGTITRISPDVRGGNTNYYVTLDTNKGIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEGTGTDENTDSQDSENQGNV
                     "
     gene            complement(15029..15592)
                     /locus_tag="PC523_002177"
     CDS             complement(15029..15592)
                     /locus_tag="PC523_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475696.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKSISDYRE
                     DYYYDTMALIYLPMNIHRHSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(15753..16466)
                     /locus_tag="PC523_002178"
     CDS             complement(15753..16466)
                     /locus_tag="PC523_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HDF2475697.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLINSQYELELRPTLQEGNLVVSLENVKLGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            16758..18422
                     /locus_tag="PC523_002179"
     CDS             16758..18422
                     /locus_tag="PC523_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HDF2475698.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPENKTEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDLPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPAK"
     gene            complement(18529..19428)
                     /locus_tag="PC523_002180"
     CDS             complement(18529..19428)
                     /locus_tag="PC523_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDF2475699.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEQNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELNIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVYIECADSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(19455..20093)
                     /locus_tag="PC523_002181"
     CDS             complement(19455..20093)
                     /locus_tag="PC523_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HDF2475700.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KVGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKSTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(20433..21431)
                     /locus_tag="PC523_002182"
     CDS             complement(20433..21431)
                     /locus_tag="PC523_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HDF2475701.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYNYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KEFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADFIQNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(21529..22104)
                     /locus_tag="PC523_002183"
     CDS             complement(21529..22104)
                     /locus_tag="PC523_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475702.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            22421..22684
                     /locus_tag="PC523_002184"
     CDS             22421..22684
                     /locus_tag="PC523_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475703.1"
                     /translation="MIKNTKQITVEVNEILLQEISKITNDENNILTDLINESIVNYIL
                     ENKKHNVEDKDLEEVIKFYKKENTEKTKSAARAIAEAKTHTLY"
     gene            complement(23104..23481)
                     /locus_tag="PC523_002185"
     CDS             complement(23104..23481)
                     /locus_tag="PC523_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2475704.1"
                     /translation="MTITKIPPAELKVMKFIWISDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
BASE COUNT         7996 a         3662 c         2472 g         9464 t
ORIGIN      
        1 agatgaaatt ttatgattac aaaatttttt agtaatatat tatcagtatt tctatatttt
       61 ctattatcac ttttaggtgt tgctggtata atattcttat gtatgatacc tatgttttta
      121 tatgatttta gttctgacaa agtaattgct ttaattacaa gaatactata tttttctgac
      181 tactttgtag tcatcttatt cttgatattt ataattaata gcactaaata ctcccctttt
      241 atacttgaaa atgtaaaaag atttaaaatg atgggatgtt gtttattagt aaatactata
      301 attgaatgtg tagttggtta taaatcaaat actagtctta taatgatatt tggagacaat
      361 agtggtggta tttcaccact tatggttatt tcttttatat ttgcattaat gtgttttgta
      421 attgctgaaa cgtttgataa agctataaaa ataaaaaaag ataatgactt gacgatatag
      481 aggtgatgtt atggcaattg tagttaatct agatgtaatg atggcaaaaa ggaaaatctc
      541 acttaaagat ttggcagaaa aaatagatat tacaaatgca aatttatcga ttttaaaaac
      601 tggaaaagca aaagctattc gatttactac tctaaatgaa atttgcaaag ctttagattg
      661 tcagccaggc gatatacttg aatatgtaga agatgaaaat gaataaaaaa gagtgtttaa
      721 aaacactctt ttttattaca atatttactt actctatcta atagttgtaa tcttttagat
      781 actctttgga gttacaacac tgctatactc taattaaata agtcaaatct atacaagttg
      841 gtgattatat gaaaagaaaa ctttcaacat attttaaagg ttatgtttta ggtttatgca
      901 tagcattttt attattttct tttatattaa aaaaattaat gatatcaaat atcattggaa
      961 tagttattgg aactggaatt gcatttttat tgatttgcct atttaataaa aaaagaaaat
     1021 aacattctat aagaaggttt ttagtaagtt tacaagagaa atatattaat ttattaaact
     1081 tagtaaatat caaatataaa tcaaaaaaat aaaattcaaa ctataatatg tttctataaa
     1141 atttggtata acttatttac ttggtttatt attgtatttg aaaaaattta ttaaacctaa
     1201 aagtttttca aaatttatga tggttttatc actatcttca gttgctattg caaagtttta
     1261 tatgttgaga aaaactaatg aaaattataa attttatcga ttagtaatat tgttttttat
     1321 tgattgttgt ttacttttta tttttcaatc atttaagcta ataaatctta ctataagtat
     1381 cattagttta tatttattct tttttatctg aggaatatgg ttagagatgt ttcttacaaa
     1441 atgttttatt tgataaattc gttaaaaaaa taggtattat agtggtagat atttgttgaa
     1501 gtttatgtat ttacccttac aattcacatt gtataatcta gacactcaaa caatttaaag
     1561 tttaattcat ttgatatatg gaatcggact ttatatattt ttaggttatg tttatataaa
     1621 agccaaaaat attctgtttt gttctataat tcataatttg gttaatagtt tagatattat
     1681 ttgtaacttt tctaaaataa cgcttagtta ttatattatt ttagaaatac ttatatttat
     1741 atcattgttt tttcttcatc ttctctttac aaaaaaatat aaaacaaata taaaaatata
     1801 aattagggta tatcaaaatt aataaaaata atatatactt atttgaaaaa ggtaacaact
     1861 ctttttagtt gttacctaat aatttatcta tactttagta ttaaatcttt taatttctaa
     1921 ctttcaagta ttaatttctg tatgtagata tgctaaactt tccaacttga gatttaaata
     1981 acttttcaat ctcttcaact attccataca ccttgttttc atactctaaa tcataataca
     2041 catttatctc acctatacta tttacactta ttgtataatg ctctttctct attatacata
     2101 tgtttccaaa gtctgacaat tcattatcta ttaattttaa ctgtcttttt atttttgaaa
     2161 aatctaatct tgaaaatata tcattaggat agaaattttt attgtataca atcatttcat
     2221 ccacccctta tcattctatt tatgatactt tataccctaa ttaataatgc attaatcata
     2281 tttttccata ttattcaaat tttcttatat aaaaacagat tgcaaatcga tttttcaatg
     2341 ttttatatta ttatgtttag atgtatgtca gtaaaatgaa agtatgagtt tttatccaca
     2401 gattttatgt aagttttcaa tacctcataa aataattatc ttccacaata aaagtatggt
     2461 gcttatataa aaatgctctt tttatcaaac tatacttttt taggagatag ttaaaacatg
     2521 taaaatcatc tagctaggag agttttatta tgtctagaat taatgtatca ttagaataaa
     2581 aattaaagta gttgagtcat attttttaaa gagtttaatt tatagtagaa aaacatttta
     2641 ttttatgtaa tcaaattaga gtatgtattc ttaaatataa ataaaatgta taaattaatt
     2701 agagaacaag gaggtaaaat aaaaaaagaa agtaagttaa ttcaattata ttagataatt
     2761 ttggaattga aagtttaaaa taataaaaac aaattacaac tggacaatac atacttaaaa
     2821 aagttacaaa tagaaataag ataataatta tattatcaca taattaagat aaatataaag
     2881 tgattcaatc tttactcact gaaaaaactt ccgttgtcca atagtgaaac atttaaattc
     2941 aatatagcta atataaaact atataaaacg aaagttatct cttaataaat gttcaataga
     3001 ctatgcttta tataccactt agttaatata aaacgcaact gcaagagtag aaataacatt
     3061 aataatttca cttacatacc acttagttaa tataaaacaa tacaaataag attttgtttt
     3121 catatgattc agaaatttac ataccactta gttaatataa aactgtggat taccagttcc
     3181 taaaaaacta aaagaaaaaa tatttacata ccacttagtt aatataaaac ctatcattag
     3241 attgtgaacc tatctctatt ttcctatatt tacataccac ttagttaata taaaactaaa
     3301 ttttgtattc ttgacattga catccaatat ataatttaca taccacttag ttaatataaa
     3361 actgatagag aaacttgaaa gcgaagatgt gctattaaat ttacatacca cttagttaat
     3421 ataaaacgga aacttgtata atcacaataa ctagcttcgt ttcatttaca taccacttag
     3481 ttaatataaa acttcacttt gatttaactc atctcttttc acacctttat ttacatacca
     3541 cttagttaat ataaaacaat gctgataata gtaaaattag ctcaatttct cgatttacat
     3601 accacttagt taatataaaa cctgtatatt tacaagtttt atataaatat tagtttccaa
     3661 tttacatacc acttagttaa tataaaaccc agttgaaagg tatcctatta tctgcatttc
     3721 ttaaatttac ataccactta gttaatataa aacttaacta aaacttgaaa accataatct
     3781 ttaagcttaa atttacatac cacttagtta atataaaacc ctaaaataaa cgtagaattt
     3841 ccaatactca cacatatgca attctcttaa atttgcagtg agcgaacagt agtgcaaatg
     3901 ataatgttta tcaatcacta tcaatacctt acatttcaat gcttaaacat attttcttca
     3961 aaaaatcgaa cactgcaaaa tttctatatc tttattatat cataaatata ttatatattt
     4021 ttgtatacaa ttactctatc atcaatagta tttataactt agatttcaaa taataaaatc
     4081 atagttatat aatgtattaa aaatacctaa tgacaaatga aaatacataa tttaagatat
     4141 acaggtgcta gtttgttaca tttggtagga actaatatga aaatagtttc tgaaagattt
     4201 tgtcatacag atttaaaact aactatgaat atatattctt atgttttaga agaaatgtac
     4261 aaaaagacct ctaataattt aagtaaaata ttattcagat aaacaaaaaa ctgactgtca
     4321 gttaaatgtc agttaaacct aaaaaaggaa tttgccagtt aaatgccagt cagttatttt
     4381 ataatatctt tgcttaaata gggctagatt tgaacacatt ttaactcttg aaactattta
     4441 ttaatataca aactttactc atacacagaa tatcttttcc aaatctgctc taaatcatca
     4501 aaatgttgtt taatatcttc tttttctttc attccaacag atatagcaag agcatttgaa
     4561 atactaagag caggaactag agaatctaca aacgaagcca tattactttt aactaaaaga
     4621 gtattgtctg caagtgatgc tactggtgca aataaactat ctgttaatga tattacatga
     4681 gctccttttt ccttagcata attgacaatt tgataagatt ttttagaata acgagggaaa
     4741 ctaatagcaa ctattacatc ttcttcattt attcttacaa tctgctcaaa agcatctccc
     4801 atatccattc taattatatg aacattgtca agtattatat ctaagtaaaa acctaaatat
     4861 tgagctacta caaaagaact tctcattcca agtatatata tttttctagc tcttaaaagt
     4921 ttattagaag cttctttaaa tgctctttca tcaatttctt ctaatgtaga cctgatatta
     4981 tctatgtcac tctttaaaac tttgtttagt attgcaaaat catcagaata atcatgtgcc
     5041 atttcaactc tttgtactgt agttagttta ttttttatga gttcttgaag tgcagcttgt
     5101 aatttaggat atccactata acctaatgca tttgcaaatc ttacaactgt agactcgctt
     5161 actcctacag tttcacccaa tttacatgca gtcataaatg caactttatc ataattattt
     5221 aaaatgtatt gtgctataag tttttgaccc ttactcaatc tcgtatattg agattgtata
     5281 tttgatatca aatgtttact gtccttcatt tcatttgttt cttccatttt tttctccttt
     5341 tagttatata aactattttg ttttattaac actgtctttt tctctaaaaa ttttattttt
     5401 tatctcaatt tcatcttagt ttttatcata gcttcttaaa ctcataaaat tctaactttt
     5461 ctgtacctta tacttcacat agtattttcg attaaattta ttataccatc tttccttgca
     5521 ttttttaata tttatccaat atttctttta aatttttcaa tttttactca tttaaaagtt
     5581 atctatctta ttttgcatat ttagatgtat attttgtttt gagtataagt tattgtatac
     5641 ttattattta aagtattttt aataggtcac actatatata ttaactctta atagaatctt
     5701 gtcaagtttt ttagtattta ttatgctttt ttcttttcaa ttaaaatcaa ttgtggtggg
     5761 ttattgactt gatttaaaaa cttagattct aaaacattaa attcatcttg atttaaatta
     5821 ttaacaaggt tatatataca atctttttct tcttgacctc catcatgtcc tgtatatatg
     5881 gcaatgctta ttactccatt ttcttttaac atttctaaac tcaactttat agcctttaaa
     5941 gttgtatctg gttttgttat aattgaatgc ttcgctcttg gaagataacc taaattaaat
     6001 aaaacacatg aaacttcttc ttttatatat tgattcattt tttcatgccc atcaaatatc
     6061 aattttactc tatctgtata tccttctttc tctaattttt ttcttgtaga ctttattgct
     6121 tcttcttgta tatcaaaagc atatacaaag cctttttctc caaccttctc agctagatac
     6181 cttgtatcat acccatttcc catagtagca tcaattacta tatcaccttc atttataata
     6241 ttttctaaat aaaatttatt tatatctgta attttagtta aatactttgc atatgccata
     6301 atactatttc tccctactat taactttatt tcttttttct caatttattt ttatcttatt
     6361 ttattgattt taaatatttt atttcatctt ctgttaggta tctatattct ccaactttgg
     6421 tgtcttttaa tactattttc cccacagaaa tccttcttaa gtttaaaact ggatgattta
     6481 tagctttaca catttttctt acttgtctat ttcttccctc atgtatagtt atttcacaca
     6541 ctgaataatt tttttcttta ttttctttag ttacctttat tttggcaggt gctgttgtgt
     6601 agtcttctat ttttagccct gtttcgaatt ttcttatttc atcaccactt ataattccct
     6661 tcacactggc tacatacgtt ttatcaattt catgctttgg atgggtaagc ttatatgtta
     6721 aatctccatc attagtaagt attaataacc cactagtttc ataatctaat cgccctattg
     6781 gatatactct ttcctttacg tctttaagta tgtctattac acttggtcta ttaaattggt
     6841 cttttactgt agttatatat ccctctggtt tattcaatac tatatatata taattttcac
     6901 ttggctttac ttttttatta tcaaattcta ctatgtcttt ttcttcatca acattaaaaa
     6961 atagctcttt tactatactt ccatttacct ttattcttcc ttcaagaatt atttcttctg
     7021 cttttcttct ggaagctatt ccacaagaag ctatatattt attaattctc atacttaatc
     7081 cttccttatc ttaaattatg tgctgtttaa tatttttatc aatattaatt ttgctacatt
     7141 attatactat actagacctt aattaaaaac aagccaaaaa attatttaat tttcctaaat
     7201 aattgtaatt tcttttcaca tactattaat atgcaatatc tgactagata ctatttaagg
     7261 aagtgattat tatttcaaaa gaaaatataa aatattattt aatagtcgtg tttattggaa
     7321 ttttattttt taaatttatt aatactccat cagactttat atctagtatt gaaggtttct
     7381 taaagttttt tagcccattt ttattagcta ttttacttgc tttactttta aatccacttg
     7441 tgatgctttt tgaggtaaaa ttcaaagcac atagactttt agctattttt ttatcttata
     7501 tatttatagg ttttatttta gcctttgcca ttaggttatt gattccatca atagctaata
     7561 cattaaatag attgataaat gaaatgccta tgtatactga ttatataaat agtttcatag
     7621 aaaaaaatat gtcaaacata gattttctaa aaactttaat tcctcatatc caacatagtt
     7681 tagataatgt attaaaagaa gcaagtaatt ttattggtaa aatttctaaa aacttcttaa
     7741 tttatacact tagcatatct tctatgctat ttaatatgac aatgggattt atactatcta
     7801 tatacattat atatgacaaa gaaaaaattg cattgggatt taagagattt ctatattctt
     7861 ctactactag aaacaaagca gatagcatta tagaattttt taggactact catgatattt
     7921 tctatgatta tttacttggt agaatattag attctcttat tattggtata attgcatttt
     7981 taggatttca atttgttatc agaatagaaa atgctttatt tttagcttca ataatattct
     8041 taggtaatat aatcccatat tttggtcctt ttattggtgc aatccctcca atagccatga
     8101 ctatattata tagtccacaa aaaactatat gggtcattgc atttctattt atattgcaac
     8161 aattagatgg aaattttata gaaccaaaag ttatgggtaa tcaagttggt ataggagcta
     8221 tctgggtaat atctgctatt ttagtaggtc aatctctatt tggtttcata ggagtttttc
     8281 tttcagtgcc aatagctgct gttgtaaaaa cttacgttga taaatatata gacaatcggt
     8341 tgcaatagtt tatctctact gttatttagc ttttagttta aattttaact gttatttgtc
     8401 aaattatttt taaaaaaata cctcttagta agaaaaactt actaagaggt attttttata
     8461 atatatattt tgttcatcaa atttatgtat atattttata cttattgatt attcgagttt
     8521 tgagaatttc cactactatc tgaaccacca ctagtatctt gtggggttgg tgctggtgtt
     8581 ggattactac tatttggctt ttcttgattt tgagaagagt tattattatt gtcagattta
     8641 tcactatctg attttttatt atttttagaa ctagatttat tatttttact acttccacta
     8701 taagaagatt ttcttgcatc tgattcaact ctatacctat cttctccagc tgtatctttt
     8761 gcctttttct ttctttgttc ctctgcttca ttttgttctt tttctaattt agttaactct
     8821 tctttctttt tagttgcctc ttctttaata acagaggaac catttttgat tttctctata
     8881 agtttaagtt ccttgattgc cccctcataa ttttttaatt cttcaaactc tgtcatattt
     8941 gacattctca tagcttgcat atacatagct cttgcattct cattagtact atcttcttcc
     9001 agtgcagagg ataatgattc catagctttt ttatagttat gtttttctaa atacgttttt
     9061 ccttgctcta ctaaatttat ttcatctttt ttattaaatg aacatcctac aagaaatata
     9121 gataatatcg atattaataa aattactctt cctctcatta aatccctcct tgtcatatac
     9181 tttaaatttt atcataatta taaagttttt aaaatatttt atatttatta caagaaattt
     9241 gttagtaaaa cgaaaaataa gcagatatgc tacgaacaat ataataaacc aaaaattaat
     9301 ttagatatat atcatagcaa atctgcttat aataaacttt aatattcaat ttttactata
     9361 tgcttctcca ttttatactt ctaaagctaa taagtcctca tagctttgtc tcttacaaat
     9421 tatcttatca tttccgtcaa atactgatac tactgctgcc cttggtattt tattataatt
     9481 tgaagacatt gaatatccat atgcaccagt tgatgttgtt ataagtatat ctccactttt
     9541 aatgtccatt atatttatat ctccaattaa tatatctcca ctctcacaac attttccagc
     9601 tattgttaca tgattcatag ccttgtgatt aattttattc acaatactac actcataact
     9661 agcttggtac aacgaaggtc ttacattatc tgtcataccg ccatctacac ttacatatac
     9721 cctaacatct tttatatctt ttatagaacc tactgtatac aatgtacttc ctgcatttgc
     9781 aactattgac cttcctggtt ctattaccaa tgtaggtact tctattccaa gctctttaca
     9841 tgaagtttct gctttagtga ttatagtttc acaaaactct tttatagttt taggtttatc
     9901 tccttcgcta taataaactc caacgcctcc acctaggtca atctctgtta gatgtatgtt
     9961 gaatttttct tttatttctt ttactaagtt catcattata tctactgtat ctatgtatgg
    10021 ttctacatca aatatttgtg aacctatatg agcgtgaaga cccacaagtt tgatattttt
    10081 atagtcactt aatttttcta ttgctctaaa taaatctcca tttactaaag caaatccaaa
    10141 tttagaatct atctgtccag tttttatata atcatgtgta tgagcttcta taccaggagt
    10201 tattctaaaa tatatctctt gagttttacc tttatcttca cataatgctt caagtaaatc
    10261 taactcataa aaattatcca ctacaaattt acctacacct aaatctaatc ccatgtttat
    10321 ttcttcttta gtcttattgt ttccatgaaa gaaaactttt tccataggaa aatttgattt
    10381 ataagctgta tacaactcgc ctcctgaaac tacatctaga cacatttgtt cttcatttat
    10441 aagattacac atataaagag gtaaaaaagc tttccccgca taagcaacct tattttttcc
    10501 ctctctagct ctaaaatatt ttatatattc tctacaattt tgtctaacta aagcttcatc
    10561 aaatacataa agtggagttt gatatttttt actaagttct aaacaactca ctcctccaat
    10621 atataattca tcattattta cagtcatagt tccatgaagt ttcataatag cctccccaaa
    10681 ttttatatat taaatacttc tgctattttc tctatagcta cttgcttatc atcagaattt
    10741 atagcacaag ttatacgaat ttctgatgta gttataagtt ttatcattat accattttca
    10801 ttgaatatct taaatacttt agcagcaact ccagaagtat ttttcatacc aagacctact
    10861 aaggaaaatt tagtgatatt attatcaata actacatgtt cttcatctgt atatttagat
    10921 actatctttt tacattctcc caattcttct tttggaactg taaatgatac acttatctta
    10981 tctaaaatag gtgcagtttg gcttatcata tctacactta tacctatagt tgctatatct
    11041 tcaaataagc tagatatatt ctctgcttta aaatctttta ttgttataga actgtcatca
    11101 tcactagtag ctaatcctgt tattacttta tcctctaatt tcatgtcttt tccccctctt
    11161 atatatgttc ctttttctgt accacatgta cgtccaacat aaatttcaac accatatttt
    11221 tgtgcaagct ctatacttct tgaatgcatt acttgtgctc ctaaacttgc taactcaagc
    11281 atttcttcat attcaatctc atcaagcttg ctagcctttg aatattttct tgggtctgta
    11341 aaataaattc catcaacatc tgtatatatc tcacattttc catttaattt aactgcaaga
    11401 gctacagcag atgtatctga accacctctt ccaagagtag ttacatcacc atcttcgttg
    11461 attccttgaa atccagttac tatcacgaca tttccttcat ctaaactatt ttttattctg
    11521 tttacgttta tatcatcaat ctgagacttt ccatgaagac cactcgtttt tatatttaac
    11581 tgataagcat tataacttat agctttacat ccaagtgcat ttaatgctat tgataataaa
    11641 gatgctgaaa tcatttcacc agtagacatt aaagcatcta attctctctt actaggttca
    11701 ttactcaatt cctttgccaa agttatatat tcatcagtgg attttcccat ggcagacaca
    11761 actattacca tttgtggatt ttcttttctt cgttctatta tgttttctgc tattgatttt
    11821 attttttcag tatctgctac tgaacttccg ccatattttt ggacaattat actcatgctt
    11881 tctccccctc aagctaaatt ttgataaata aaaacgccca aaggtatact ttggacgtgt
    11941 aaatttcata gaccaagtaa ccttgtagct caccacttta aaagtgacag ccttatgcat
    12001 attctgcata agtccagaaa atttgtccgt caacaaaatt tcttcggcta tatacccttt
    12061 catcaattat caatgcttac cagctcctat tgaatactat tgaatacagc tacctctacc
    12121 ctaggctcat tatttttaat taatttatta atatagtttt atcatattct atataatatt
    12181 gcaacaatta ttttacaaaa tgtggcaaat caatagtttt cttttctaaa attagcttat
    12241 atacaattaa atagtttatt taatcccaaa tctataaaat ttatactata ctcattatca
    12301 tatctctcta aatcatttaa ttcagataag ttatctggtg taactaccaa aacgtttggc
    12361 actttaaaaa atggaattgt agatttatct atttttttag aaagattttc gtatttagaa
    12421 atgtctattt cattatttaa gtcaacatct actagaactt gctttatatt accaaattgg
    12481 tctgtatatt caataaataa atcgtattta acatcatcta cactaacatt tcggtaaaat
    12541 ctttttacat cacaacctgc agtattgatt ctaacaacta attctgatcc tactagagcc
    12601 tttattaaat catctccctt aaaaagctta ctaccattta aatagtatac ataagagagg
    12661 tctttataat tataataatc tacatcacaa ggatattttt ttagtgtata ttcactacac
    12721 atttttctca aagttttttt aaaatttttt ttattgtatc ttttttggtc tccataaatt
    12781 tttctaaatt gagactctgt aacacatcta accctgctaa ttaaaagtaa tatctctttt
    12841 tccatattcg tcatagtaca ttctccttga agtattattt gcaaagttgt actaatatta
    12901 tattaatttt atgacattaa atttacaaag aattaaaata aaatagctaa tttttataga
    12961 attaaattct ataaaaatta gctattacta tgttaaatat tgttataagt ctttttattt
    13021 aaattataca ttaccttgat tttcagaatc ttgagaatcc gtattctcat cagttcctgt
    13081 tccttctttt gcttttttat cttcaaagtt tgcaatattt agattatcaa attctgacat
    13141 ttctattaca ccagattctt cttttgaata cgatattttg actttatcac caacctgagt
    13201 caatggcaat tcgctagata cttttgaagt agccctaaat attatacctt tatttgtatc
    13261 taaagttaca taataattag tgttaccacc tcgtacatct gggcttattc ttgtaattgt
    13321 accttctatt acctcatctt ttaattcatt atcaagtttc aaatcattcc ctttagattc
    13381 taatgcatct ttatagtttc ttaatgcttc attcttatct tcacctattc caagtacatt
    13441 ataatcttca acagatacaa atgcaactct cttaacaaga ccagccttat cttttaatga
    13501 tgatacatat gttggctgac ccaatatatt atacattaca gggaatgttg cttcataatt
    13561 tttttcttga acattacctt cagctgattt cattgcagcc gtttcagtag ctcctggttg
    13621 tttatataat ctagcttctt ttgttctaga atcaactaac ataaatccaa ttgtagattc
    13681 atctccacct gaggatgtta tacctgtata ccaataagac ctattatcat taccataaac
    13741 taatgatgta ccttctgttg gtactaaaac tcctttttct gatataacag aatttaaaaa
    13801 tccattaaca taaacgcccc agtctttaat ctggtctgtt ataaagtttt gtggttgtat
    13861 tctatccacc catttaggtg tgtttttaac atcataaaca tttatttttc cagtttctgc
    13921 atcaacagtg gctattcctg tggcatttgc tcctccatat ccaattttat gttcatataa
    13981 acttacaacc caataaggtc ttccttcatc atttatttca agagtgaaat ctgtcatacc
    14041 aacatttaca ataccatgta tgtataaatg tctttgtaaa tcttgatgta aataggcttc
    14101 tggttggtaa actatcttta ttggctttcc atctatttct tgaactaatt ggacatcttg
    14161 aggattacta gctgatactt ttacataacc acttgtacca cttagagatg ttatccattt
    14221 aattatgtcc ctgtgaacaa gaggtgctac ccagtacagc tctccatcta cattttgtat
    14281 atgaaatttt cctaatttgg atacactccc tattgcagga acttctccaa gctttttatc
    14341 gcccaatttc attgccatat cttcatctac aagacgaatg tcatttacac tgacagggct
    14401 aatgtcatca gtaaatttac tttcagttac ttttcccaat agttctctat atgattttgc
    14461 atgaaataca ggagtagaag ttataaaggg aactaccaca atatacacaa gcaaagctat
    14521 tgctattgaa aaattatatt tagccatttt agatgctctt tcatttctat ctaacatcat
    14581 atctagtatt cctgctacaa caaagaaaat tattcctact gaaaataaag atgtaaaatc
    14641 taatcttagt acaggtaatt taataaatgc ataaattatt acaattatta atgcaactcc
    14701 atatgtttta agatatcgtt tcatttaata ctcctctcta aaaaatgtta ttattactat
    14761 tatatccata tatttacaaa aaataaatat ttatcatatt tattatgtat taagatatct
    14821 ttatatattt atagttctac ctattcaatt atatgtaata tatatatcca aaaatattat
    14881 aaaaatacaa ataatagcag aaatttaaaa actgaagaaa gtattctaac taagatttac
    14941 aaattcttaa tcctatcgaa gttaaactac tatcttcagc ctaattgtct agcaatattt
    15001 tttatatgaa aaactctaaa tcaagttttt aaatgtttgc gatttttgat acataagaat
    15061 gttgtctaga attatattct gataataaac cttcatactc taaggttaaa ccagataaat
    15121 tataaaaatc atttggtgtt acttcaaatg cttgtttatt taaaatgtct atctcacttt
    15181 tttctaacat gtcagcaaca aaatttgaac atacatattt atattcagag tgtctgtgaa
    15241 tattcattgg taaatatatt aatgccattg tatcgtaata gtaatcttct ctataatcac
    15301 taattgattt tatattttta tatagatttt catattgtaa attatctact tctaacgaat
    15361 ataccctaca cacagtgttt tttcttatag catataatcc ttgatttata ttttcttcaa
    15421 caaaacctcc aataaaggga ttccttggat ttagtctccc aaaagaatac attggtttta
    15481 gattttcatt taatgatatt gacacatgtg tatacagttt tttacttata ttccttatta
    15541 agtaagataa tatagttcct gtatatgttg ttacaatata gactttattc atcatttccc
    15601 cccaatctag taactaaata atattataca tttacaattt aatatcataa gatattctaa
    15661 cataataaat gtagttttac aaattttgat tttaaggtac tttaacatta tataaaatat
    15721 aataaaaata taaatataag ttttctatat ttttaaccaa cagacgtttt cttcaaatta
    15781 atcagatgat ttagtatatc ttgaaccttc ccagaatcct ttaaatatcc taatgccccg
    15841 tttatttgtg attttaaatc tatttctact tccatatcta agtcaacatt tccttttttt
    15901 atagatatat ttttaagcgt tattgggtaa agataacttt tttctaaagt aatcttattt
    15961 ccattaacct caaatggaac ttttttattg tagttactta atattttttc taacatttta
    16021 tcacttagct taaattttcc aagtttcaca ttttcaagac ttacaactaa gtttccctcc
    16081 tgtaaagtag gtctcaattc aagttcatac tgactattta tcaaaccaaa taccttatat
    16141 ggtcctgcta cctttatttt accatttttc atttcaacaa aattattttc aagttcatta
    16201 ataccatgtt tctgcatcaa tgtatagatt aaatttttaa actcttcctc tgaaacagat
    16261 atttttccaa ccattctaag tggatttttt actacttcca tagaatttat atatgaacct
    16321 tttgataaat attcttcatc tataatttta ttattttgtg aactaacatc atatctttct
    16381 tttggtgtca agatatatac aacaatacct gcaagtacta aaacaataac aattaaactt
    16441 aataaaatct ttgcaacctt tcccataatc gtctccttta caaatctgtc aacagattta
    16501 aaataaattt agataactaa cttcacataa tattcttaac aatatacata ctataataca
    16561 gtcttttaaa aattaattca actaatctca ttttttgtaa ataaactgta aaaacatgtt
    16621 tttagtataa aagttaatat tttgcataat actcttaatt acacctttat ttttttctaa
    16681 tcttggatta aaattaatat tatgcaatac agtgtagtgt tctgtattat aatcaaatta
    16741 aataggaggt atagaatatg tcaaatgaaa cgaactcatc taactttata aaaaatatta
    16801 ttataaacga cctagaaaca ggaaaacatg acagtataat aactcgtttt ccacctgagc
    16861 caaacggata tttacatatt ggtcatgcaa aaagtatatg tcttaatttt ggattagcta
    16921 aggaatttaa tggaaaagct aatttaagat ttgatgatac aaatccatta aaagaggatg
    16981 ttgaatatgt agaatccata aaagaagatg tcaagtggtt aggatttgat tggaacgagt
    17041 taaattttgc atctaactac tttgatgaaa tgtataagag agctttaata ctaataaaaa
    17101 aaggtaaagc ttatgtatgt gatttgacac aagaagaaat gagagaatat cgtggtactc
    17161 taactgaacc aggaaaagaa agtcctcata gaaacagaac gattgaagaa aaccttgatt
    17221 tatttgaaag aatgaaaaat ggtgaattta aagatggtga aaaaacatta agagccaaaa
    17281 tagatatgtc ttctccaaat ataaatctta gagacccaat tatatataga atatctcatt
    17341 caacgcacca caatacagga gataaatggt gcatatatcc tatgtatgct tttgctcatc
    17401 caattgaaga tgcaattgaa ggaataactc actctatatg tactttagag tttgaagacc
    17461 aaagacctct ttatgattgg tttgttaaag aatgtgagat ggaaaacata cctagacaaa
    17521 tagaatttgc tagacttaat ataaataata ctgtcatgag taaaaggaaa ttaaaacaac
    17581 ttgtagatga aggtattgtt gatggttggg atgatcctcg tgttcctaca atatctggaa
    17641 tcagacgtaa gggatatact gcagaagcat tgcgcaattt ctgtagtgaa ataggtgtat
    17701 caaaagtcaa ctctacagta gatagtcaaa tgcttgatta tttcctaaga gagaatttac
    17761 aaccaaaggc tcctcttact atgggagttt taagaccttt aaaacttatt ataactaatt
    17821 acccagaaaa taagactgag atgcttgaaa tagagaataa tgctaaagat gaatctcaag
    17881 gaaaaagatt agttcctttc tctagagaat tgtatataga acaggatgat tttatggaag
    17941 aacctgttaa gaaatatttt agatttttcc caggaaatga agtccgttta aaaggtgctt
    18001 attttgtaaa atgtactgat gtaattaagg atgaaaatgg aaatgtagtt gaaatacatg
    18061 gtacctatga ccctgagact aagagtggct ctggatttac aggacgtaag gtaaaatcta
    18121 caatacattg ggttgatgct aaatcagcta taccatgtga atttagattg tttgaaccat
    18181 taattcttga tgatttacct gaaaacgagg gcaagcattt cttagaacaa ataaatccta
    18241 attcattgga aatattacaa ggttttgttg aacctacaca gataaaagat gctaaacctt
    18301 ttgataaatt ccaatttgtt agaaatggat tctttagtat agataataaa tatacaactg
    18361 atgaaaaatt tgtatttaat agaatagttc ctctaaaaag ttctttcaaa ccggctaaat
    18421 aatcttacaa tcaaaaaatg aagacttaaa atataaatta cttctactat aaagaaactt
    18481 agttagaagt aattttttat ttcagccttc attatttact ttaaatgatt aacttttttc
    18541 actacagatt cccataactt ctattccatc ttttatacat tctatgttaa ggggaaaatc
    18601 tggtcctttt tctccatcaa tatccgttat aagtgaatct gcacattcta tatacacctt
    18661 tttagtttta aagtatagta tctcatttcc attatattga tctaaataat cacctctaag
    18721 cacacttatc aaaactggta ttgattttgg tattggcatt cctttaaaaa taatcacatc
    18781 cagataccca tcatctattt cagccttata tgctagattt atatttcctg ctgttttgcc
    18841 attaaaaatc aacataagat acatatctcc atcataatac atttcctcag aagatacttt
    18901 tatcttaaat tttctcatat acaatgcttc ttctattccc tttatataat aagaaattct
    18961 tccaaatgaa ttttttaagt ctgtatttat cctttgtgat acatctgtaa acataccagc
    19021 gcttgcaaca tttataaagt acttattatt gactttccct atgtctattt tcttgggttt
    19081 tgaatttatg atttcattta tagcttcttt tatatcaaat gaaatttgaa gagcatttga
    19141 aaaatcattt gcagttcctg taggcaaaat tccaattgga atttttatgt ttaattcttt
    19201 cattgcattt actactaaat ctacagtacc atcaccacct gctatcaata tgtgttcata
    19261 agaactatca acttccttaa atgcattctt tacaggttct ttaatagtca atctataggg
    19321 tatcaataag taattttgtt cttgatataa ttctatgata gtgtctaaat ttgataatat
    19381 ctttttttca cctgaattag gattgtatat aagttttact tttttcatta tttctcctta
    19441 ctcattttat tcttttagta tccaaaatct tcattcaaaa aaatcacatg tattctttct
    19501 ggtgttaatt gtctttttat aagtgtataa ctacagcata ttcttcctgg acttgaacag
    19561 tctatacata taccagtact cttacatcca gtatttgtac ttaatctttt acaattggct
    19621 ggagcagcta ttgtttttgt tctttttatg gcttcatcta catctttaac tattttattt
    19681 actcctgcta taactatcac tttatcaggt ccatatatca tggcagcaac tctattacca
    19741 ttcccatcta gattgtaaag ttctccatcc tctgttattg cattagtact tgtaaaataa
    19801 gcatctgcag aaaatgactt tctgtatatt tctttcattt cttctggttt gatatctttt
    19861 gcatatctgt caagaaaatt atatttttct cttataaaat caatcactcc tgtttcaaat
    19921 aaggtcatag aacctccaca tcctactaga gaaccaactt taactatttc ttcaattttt
    19981 ttaagaagct cttctttatt acttactaaa taccctttca tattattatt ttctaaagat
    20041 tttattgttt tttctattct tttttcatta agccaattta aattttgatc cattttaact
    20101 tcccccttat ttttaatatg tacctaatta tttataaact ttattttctt tataagttta
    20161 ttttagtaaa tctaaaaaaa aatacaatat aaaacttaaa ttgttttcca taattttttc
    20221 caaagtaact tactcaatca aaaaaggaaa gtagatgtac tattctactt tccctatatt
    20281 gtatgtagta ttatatattg cttaaaatct atatattaat tatatgtttc atattataga
    20341 atatcacagt taatttaaat gtaatactat attcttttca aacatataaa ttgtattatt
    20401 aaatttaata tatccaactt cagtaagtct tattaatttg atgaagaaga tgctatagct
    20461 gcacttgtcg ctgccattac acatgcaatt tgaatagcaa caacaatatt taaaaatata
    20521 ttattagtta tagagcctat attattctca ttttgtatga aatctgcata aattgaagct
    20581 acaatagcag aactaatcat tactctatta ctttttccta aagaccaatt tccaaagcct
    20641 ttttcttctt taagtttatt tgaaacattt tttatctctt ctattatttc atcttctcta
    20701 taatctaata gtgaaattgc tcctattatt ggatatccat aataattcat tttacaatca
    20761 ttttctttaa attctttttt gagttcaata gtctttttac atttaagcat agtcctatca
    20821 tcactaaata aaagaatttg ggataatgct tgtaaatcat ttcctttata aaaaccattt
    20881 tcatttagaa attcataaca tttttctatg tattctaaat cttcgtctaa attttttgaa
    20941 tgaattgcaa taagtgctat attacaatag tcatcactag aagttagaaa tggatgtttt
    21001 ttcctcatat agttatatat tattttcatt tttgatatac atgtaagata atccattttc
    21061 tctctatttt cataaactat tgatgctgta agtggtaaat atgtatcgcc ccaaaatttt
    21121 tcacttttaa gttctttgta tatatctaaa atcatcttaa aactactttc tggatttgat
    21181 tcaaatgaaa gtagtgttgc catataaaac atattatatc ctctaaaatt agagaataat
    21241 ccagtatttt ttttaataag ctctattcgt tcttctacta gtctaggact tattctctta
    21301 ttcttaagag taaatgcaag tgcacaacta tgttgtatca atcccatttg ccaagaacct
    21361 ttaacttcat ttaatgcttt gtaattttct attagtaagt ttacttttcc atttataaaa
    21421 gactgttcca tattgccctc ctagtaatat atctcattgt attaatagtt ttatatttat
    21481 tatatatata ttaaatatta caatcaatac atattacttc aaagagcatc attcttcaac
    21541 aataatgtaa ccttttttgt aatgtcttcc catattattg actctatcat agtactctga
    21601 ttcactttca ttactccatt ttggcacaaa tacatctatt gcattagaat tgctaaaata
    21661 cttactagac cccctatcca taacagtttt taattttcca tctacaataa tcttagttcc
    21721 aaaactcaag tgattgtttg ctacaaaacc atcttctaaa tcatctcctg tagcagttat
    21781 cgctccatat ccattttctt gattgagact tgtgtaataa ctaacttcaa aagttatagg
    21841 tactagcttc tttattttaa tacctaactg attttgtaat ttctcctttt gtactttcag
    21901 cttatactca tcactatttt tatagtcttc cataccttga ttatacttgg aattaaagct
    21961 tttacctgac tttactatag tatcgatatt tactatagat tctttacaat gatctgcatc
    22021 tatttttgtt tctccattta ccaataatgg agttaaacat acactacata caacacatgt
    22081 aatcaatgct tctttaagct tcataaattc cccccttatt attttgatat attagcatat
    22141 ttaatattat atgtctacat ttagatttaa ataccacacc catttgcttc aagaagttgt
    22201 cttatattta attcacctta aacacttata aatcctttat ctcagaatca tatagaataa
    22261 agctttttct gttaaatata ttttgttaat cttttaaaac ctaaaaccac ttttatttaa
    22321 ttttctattt tttagaattt aagaattaaa attctataat tttaaaacat attgaattta
    22381 tttttttgag atgatataat ttttttaaga ggtggttgat atgataaaaa atacaaaaca
    22441 aattacagta gaagttaatg aaattttatt acaagaaata tctaagataa ctaatgacga
    22501 aaataatatt ttgacagatt tgataaatga atcaattgta aattatattc ttgaaaacaa
    22561 aaaacacaat gttgaagata aagatttaga agaagtgatt aaattttaca aaaaagaaaa
    22621 tactgaaaaa actaaaagtg cagcaagagc tatagctgaa gcaaaaactc atactttgta
    22681 ttaataaaaa aaagttccta aaaattagga acttttttat tttaataatt attaatatat
    22741 ctcaatattt atcaatataa acatgtttaa tacaatctat tgtatactta tcttacccta
    22801 gaaataatca aaacaaaaat aaaattatgc cctttatatc taaaacaact aatttacatg
    22861 attaatatac aaaaattctg acttttaaat tcgtatacaa tatcaaacta aaataatatt
    22921 aatgacccca atataaatta aattctaaca ggtttgattt tacatcaaac ttattgtatt
    22981 aatattaaat aacaagacaa aaatacttgt aaaaatcaca aattttacat aaaactttat
    23041 atttgattca tttaactaat tattgtaaaa actatttatt ttatgataat taactacacc
    23101 aaattattca tcttttttaa tatctttaac ccatttttca acagagttca attcttcttc
    23161 atttaccccc tttttgaaca aagatttcat aaaactctca atagaacttc catgcatatt
    23221 atctaaaaaa gtttttgttt caaaatgtaa atactcttct tcgccaataa gaactgtata
    23281 atgtgtatgt ctatcaatct ttcttgcact taagaattgc ttatttacta atcttgataa
    23341 aagtgttagt gttgtagttt gcttccaatt atatagtttt tccatagctt caatgacatc
    23401 ttttgatgct actgtaaaat ctgaaatcca aataaatttc atgactttta attctgctgg
    23461 tggtatctta gtaatagtca tatgatttta cactcctctc aaacttctac ataattaaat
    23521 actatattaa tagacttttc gagtcaaagg ttttatttaa aatatctcat ccaaatttat
    23581 taatacaaaa aatt
//
LOCUS       DAMJVM010000051        23527 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.052, whole genome shotgun sequence.
ACCESSION   DAMJVM010000051 DAMJVM010000000
VERSION     DAMJVM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23527
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.052"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            115..453
                     /locus_tag="PC523_002186"
     CDS             115..453
                     /locus_tag="PC523_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HDF2475705.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(578..1867)
                     /locus_tag="PC523_002187"
     CDS             complement(578..1867)
                     /locus_tag="PC523_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2475706.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPSIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKNLPQIDGSSMYLKEGE
                     SFTVKQLLNALLVHSANDAAFVLARYVGGGDVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELVYEPKNSYRIFLEKNESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNIYNNGKLENSIDLIAKNNLDSSIPFLTGNNILMT
                     FVKIIAGILVLLVLFIITSNIKRKKRKKARSKRNMKK"
     gene            1947..2651
                     /locus_tag="PC523_002188"
     CDS             1947..2651
                     /locus_tag="PC523_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDF2475707.1"
                     /translation="MKKKVKFISFFILFILIISSIIVYKSLIFKDDIYVVSKKVSSNE
                     KTHAVEAEIKNEDNSHNKATKDIDKNFQNNKITIYISGAVNRPGIVTIEPDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEAISVTSNDDSNKSNQKNESRSEPK
                     NKSNKNISNKSKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            2743..3789
                     /gene="selD"
                     /locus_tag="PC523_002189"
     CDS             2743..3789
                     /gene="selD"
                     /locus_tag="PC523_002189"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2791..2793,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDF2475708.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFAKVKESGALLVGGHTVDDKEPKYGLSVSGIVNPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFDK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEIVGNVEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            3807..5219
                     /locus_tag="PC523_002190"
     CDS             3807..5219
                     /locus_tag="PC523_002190"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDF2475709.1"
                     /translation="MIILSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDISNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVIAITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVDELRKAFN"
     gene            5333..7216
                     /gene="selB"
                     /locus_tag="PC523_002191"
     CDS             5333..7216
                     /gene="selB"
                     /locus_tag="PC523_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDF2475710.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNESSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIENGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKKALETLISGDKVFIIGNMYMHVNQYNKLKENTIKLLS
                     EYHKKYRLRKGMLKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNEKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNTLINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            7321..10059
                     /locus_tag="PC523_002192"
     CDS             7321..10059
                     /locus_tag="PC523_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HDF2475711.1"
                     /translation="MKSIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDTDKYETLL
                     HTIYTIFLVATGVIVFNTTKFKVDSFSTFLGLIFIATGALECIYLFNSLEIKTQFVRE
                     LNITISAITDLFPILGVYLSFKFVKDNKQIYSSIILFIVTVTLTIISLFIISGICEYL
                     GNGTSQYALEVIISIFIIIISIVSGIELNLSSSEGKWEYNEKKIFNRIIVIIILSRVP
                     NLLHIVIDDRNIERILSHIIINVAIYYLYNYIVSKNIKKTALELHDINEELTLKTESL
                     KEKNKKLIYETEKIEELKGILIKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESINYVFVYEKNVEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSREFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTDKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYNDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRSEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTELEEINIYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVISEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(10178..11122)
                     /gene="manA"
                     /locus_tag="PC523_002193"
     CDS             complement(10178..11122)
                     /gene="manA"
                     /locus_tag="PC523_002193"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDF2475712.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGIYKGKKLSELWDKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVDMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNLGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFRCTTFVSNEFFSVYKLDIFGKCTFTHKTPFSLYSVLDGNGK
                     LIHNAVEYNLKKGMHFILPNNFGEFGFEGNLEIICSHI"
     gene            11295..12656
                     /locus_tag="PC523_002194"
     CDS             11295..12656
                     /locus_tag="PC523_002194"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDF2475713.1"
                     /translation="MELFKEEIKKYEKEFLKLLEEWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLLSNIDDKSLYISGGIEFNTIPDKVYI
                     KNIEKLGKDNICFDINKISISYDNDNYIVHGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSARYPEIL
                     SIEEIMKKFNLYMEKNNINKFELIGENLKQANYIDKNSKLVKSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHGANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            12744..14633
                     /locus_tag="PC523_002195"
     CDS             12744..14633
                     /locus_tag="PC523_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2475714.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIDSSRGIGYKLSGSKNNIARAIKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIYCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIESLMKEISDKIGFKLIEDEVIYISNYSGV
                     IACKGTQGIKNTSELEERINSVMSSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFNIKANTK
                     EEVILSLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            14648..15103
                     /locus_tag="PC523_002196"
     CDS             14648..15103
                     /locus_tag="PC523_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2475715.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKELNLAEDESLVY
                     EGLMSREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKDFKDSLLKSDNVEEISNLINEALGL"
     gene            15161..15478
                     /locus_tag="PC523_002197"
     CDS             15161..15478
                     /locus_tag="PC523_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2475716.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            15503..16765
                     /locus_tag="PC523_002198"
     CDS             15503..16765
                     /locus_tag="PC523_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2475717.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKKLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAVWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            16870..17934
                     /locus_tag="PC523_002199"
     CDS             16870..17934
                     /locus_tag="PC523_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2475718.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     EAQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIIDFIKVGMTE
                     KQVENEIVRVIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITKDGCMRLTTSTKDLIVVS"
     gene            17988..19166
                     /locus_tag="PC523_002200"
     CDS             17988..19166
                     /locus_tag="PC523_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HDF2475719.1"
                     /translation="MYNFDNKPDRVSEKCRKWDLNIIRDKFGDIREDFVPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVDVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEAQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTKVATICKENNVILVADEVHAEHIHYGKFNS
                     ILKIEKELLENVILLTSPNKGFNLGGLKTSYSIVINKNIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLNGVNEYIKSNYELLETWVNKYSKIKLMKMESSYLAWMDISGL
                     GISASEFTDRLAIDTGVLLEDGSHFVQDGDKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            19362..19559
                     /locus_tag="PC523_002201"
     CDS             19362..19559
                     /locus_tag="PC523_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005809912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2475720.1"
                     /translation="MKNCVEALRKKKGLNQEDFAKILKVSRQTISSIENGKYNPSLEL
                     AFQISDFFEMSIEEIFLWKEI"
     gene            19563..19997
                     /locus_tag="PC523_002202"
     CDS             19563..19997
                     /locus_tag="PC523_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerC"
                     /protein_id="HDF2475721.1"
                     /translation="MEKRNLRIGIVYVVLGVTFLFSCLMTKDKLSSLLVGLAAGFGFN
                     GISIIYRYFYWNKHKEEYREKLEEEKINLQDERNVMYRDKAGRYAYIVCMIIIPISAF
                     VFAVLNALDIYNSLVIIIYLSVLWIVLYVVGVIYYRKLKNNE"
     gene            complement(20173..21228)
                     /locus_tag="PC523_002203"
     CDS             complement(20173..21228)
                     /locus_tag="PC523_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDF2475722.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSGQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVKVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLNKENMKIDLGAIAKGYAADKIVSYLKSEDIKKGLVNLGGNIFTLEDSKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMDLINRIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKAFSIEKLNK"
     gene            21295..21666
                     /locus_tag="PC523_002204"
     CDS             21295..21666
                     /locus_tag="PC523_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDF2475723.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            21703..22377
                     /locus_tag="PC523_002205"
     CDS             21703..22377
                     /locus_tag="PC523_002205"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDF2475724.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            22625..23464
                     /locus_tag="PC523_002206"
     CDS             22625..23464
                     /locus_tag="PC523_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDF2475725.1"
                     /translation="MNFKKLSNVKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDKNAKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
BASE COUNT         9407 a         2475 c         4029 g         7616 t
ORIGIN      
        1 gttattaaaa tgataattaa tatcaataag tattgacaaa attattttta gatactatta
       61 tatataatat aaataataat gaaaatcaat gtcaattaaa tgggaggaaa tctaatgaac
      121 ttattagtga ttggtggtca tgaaagaatg gaaaaggatt atatggttat ggccaagaaa
      181 aaaggttata agacaaaggt atatactact atgtcatcta aattaaataa ttctatagga
      241 agaccagatg cagttgtcat acttacatca actgtttcac ataagatgtc tagaacggtt
      301 gagtctcaag ctaaaaaaca agatatatta atcgtaaggc ataaaaatag cagtaaagta
      361 gcctttaatg aatgtttaga tatggttgat gaatgtctgg gaaattgttc aaaatgtaag
      421 cttaaagata aacaaaaaca aaaaaatata taagtataaa aaatacataa acataaaatt
      481 aaaggtactg agataatagt tataattatt atctcagtac ctaattttat aagtagaagt
      541 atattataaa atttttatta taatttctaa ctaattgcta ctttttcata tttcttttac
      601 ttctagcttt ttttctcttt ttcctcttta tatttgaagt tattataaat aaaactaaaa
      661 gtactagaat acctgcaata atcttaacaa aagtcattag aatattattt ccagtcaaaa
      721 atggaatact gctatcaaga ttattttttg caattaagtc tatactgttt tccagtttac
      781 cattattata tatatttaaa gtaccaacct tagcaccttt ttttataggc aaatcaattt
      841 tatttaactc tgttttggta tcatagttgc cttgagattc atttttttct aagaatatcc
      901 tataactatt tttgggttca taaaccaatt catgctgttt tgtaaataat acttttttag
      961 tatctgtgta tttttcttta tcaacaatag tagcacactt aaagttatca aatccataat
     1021 caataagaat acgtgagtct aaatataaat cagcattagt tgaattaaat acagcaacga
     1081 taactcttct gccatctttt acagcactag ataaaagaca tttaccagca gcatcagtat
     1141 atccagtttt tataccatca actatatcat attttatatt tataggttga cctttgtatg
     1201 taatcttatc atgtgatgtc aaaaacttat tagtgtttac aaagtatctc tcataaggat
     1261 atgcttttgt tgcttcaaat ttaagtgact tagtttttac aatttgtcta aaagtatcat
     1321 tattcatagc ttctctagct atgagagcca tatcatgagc tgttgtgtag tgatttgggt
     1381 ctggtaatcc atgaggatta ttaaaatgag tattagtagc tccaattttt tttgcctcag
     1441 agttcataag gtcaataaat ttttgtacat cgccaccacc aacatatcta gctaatacaa
     1501 aagcagcatc attagcagaa tgaactaaaa gagcatttaa taactgcttt acagtaaaag
     1561 attctccttc ttttagatac atactagaac catctatttg aggtaaattt ttaatttcta
     1621 ttacttgatt taaatcttta acctccttta aaactaaaca agctgtccat actttagttg
     1681 ttgaagctgg ataaagctta gaatttccat ccttattata taatattttg ccagattcat
     1741 agtccattaa aactgcatgt ttagcaacaa tagatggttc attatctgca aaagagatat
     1801 tagagcttcc taaaaatgaa gtaaatatca ataaacagat taataaaagt gataaatttc
     1861 ttttcattgt gagcctccaa attttaaatt catcttttag tatagcatat atttgtcaat
     1921 aattaaaaga aaggagtgat taatatatga aaaaaaaggt aaaatttatt tcatttttta
     1981 ttttgtttat tttaataata tcatcaataa tcgtttataa atcgcttatt tttaaagatg
     2041 atatttatgt tgtaagtaaa aaagtttcgt ctaatgaaaa aacacatgct gtagaggcag
     2101 aaattaaaaa tgaagataat agtcacaata aagcaactaa agatatagat aaaaattttc
     2161 aaaacaataa aataactata tacataagtg gagcagtaaa tagaccaggg attgtaacta
     2221 tagagccaga caagagactt tatgatgctg tagaattact aggaggaact acaaaagaag
     2281 ccgatttaaa tggagtcaat ctatcagtaa gattagaaga tgagcaacat tacataatac
     2341 ctaaaatagg tgaagcaata tctgttacaa gcaatgatga ttccaataaa tcaaatcaaa
     2401 aaaatgagtc aagaagtgag cctaaaaata aatctaataa aaatatatct aacaaatcaa
     2461 aagtaaatat aaatgtagcg actatagaag aattggattc tctacctgga gttggagaag
     2521 ctacagccaa taaaatttta caacacagag aagaaaatgg acagtttagt tcaatcgagg
     2581 aaataaaaaa tgtaaatggt attggagata aaaaatatga aaatataaaa gatttaattt
     2641 gtgtagacta aaatataata caaaacatac ttatagatat ggtattataa atatgtatgt
     2701 atatgtgtgt atatgtggaa atttagtaaa ggaggcactt ttatgaaaaa agaacaaaaa
     2761 aaacttacag agatgacaac agcaggtggt tgagccgcta aaatagggcc agaggttctg
     2821 gcatcagttt tatctcaatt gcctaaaaat gataatatag aaaatttatt agtaggacta
     2881 gatacagcag atgatgctgc agtttataaa ttaaatgatg atatggcact tattcaaact
     2941 ttagacttct ttacaccaat ggtagatgac ccatatgtat ttggtcaaat tgctgcatca
     3001 aattcactat cagatgtata tgctatggga ggcaaacctt tggttgctat gaatattgtt
     3061 tgttttccat catgccatga tatggatgta cttgcagaaa tattaaaagg cgggtttgct
     3121 aaagtgaaag aaagtggagc acttttggtt ggagggcata ctgtagatga taaagaacct
     3181 aaatatggtc tttcagtttc tggaattgta aatccaaata aagttttatc aaatgctact
     3241 gcaaagcctg gtgataagtt aattataaca aaaccaattg gagtgggtgt attaaacaca
     3301 gccatgaaag aaggtatggt agaacaacat atagcagata aagtaataga aataatgata
     3361 catttaaata aatatgcagc aatgagtttt gataaatttg atgtaaattc agttacagat
     3421 ataactggtt ttggtctact tggtcatact ttagaaatgg ccaaagcttc cgaagtaagt
     3481 attgaaattg aatcaaaaca tgtgccaatt attgaaggtg caatagaaat ggcacaaatg
     3541 ggaattatac ctgctggtat gtataaaaat atgcattatg tatctaaaga tgtagagatt
     3601 gtaggaaatg tagaagttgc agttcaagat attttatacg acccacaaac atcaggaggg
     3661 ctgttaattt cagtaaaaga agaacttgca gaggagcttg taaaagatat gaaattaaat
     3721 ggagcaatag aagctaaaat tataggttct gtagttccaa aaggggaaaa atatattaca
     3781 gttttataaa atattataga gaaagagtga ttattttgag caaaagagaa ttgtttgcaa
     3841 tgttgccttc agttgatgaa gtattaagtg ataatagaat tgtagaaata ataaatgaat
     3901 atcctagaag tttagtttta gaatctgtga gagaagtaat agatttaaaa agacaattta
     3961 tattaagatt aaaagaagat atatcaaatt ctgtaacaat agagtttggt gaaataatag
     4021 aatctgcaat agagagagtg aagttaaatt actcattatc tttaaaaaaa gttataaatg
     4081 caactggtac agttattcat acaaatttag ggaggtcttt acttagtgaa gatataaaag
     4141 atgaattatg gtgtgctgct tcaaggtatt caaacttaga atacgattta gataatggag
     4201 aaagaggttc aaggtactct catttaacta gtacaataaa aagacttaca ggagcagaag
     4261 atgtactagt tgtaaataat aatgcagcag cagtacttct tgtacttagc acaatggcta
     4321 aaggaggaga agcaattgta tctcgtggag aattagttga agtaggtggc tcatttagga
     4381 ttccaagtat aatggctctt agtggtgcag aattagttga agttggttca actaataaaa
     4441 ctcacctgaa agattacaaa gaggccatta cagaagatac aaatgtgctg atgaaggttc
     4501 atacaagtaa ctatagaatc atgggattca cagaaagtgt ttctatagaa gagttagtta
     4561 atttaggtaa aaaatataaa ttgcctgtaa tagaagactt aggaagtgga gtatttatag
     4621 atttatcaaa atatggttta tcttatgaac caactgtatt agattctatt cgacaaggtg
     4681 cagatgtggt aacttttagt ggagataaga tgcttggagg tccacaagca ggtattatag
     4741 ttggaaaaaa agaatatata gaaaaaatga agaaaaatca acttactaga gcccttagag
     4801 tcgataaact tacaatatgt gctcttgaag ctacattgag aatgtattta gatgagacaa
     4861 aagctataga aaatatacct actttaaaaa tgttgacgta taaaattgaa gagttagaag
     4921 taaaagcaaa taaattattt gaaaaaataa ctgctttaaa cttaaatgca aacataaata
     4981 tagaagatgg attttcacaa gtgggaggag ggtctatgcc tcttgaaact ataagtacaa
     5041 aggtaatagc aataactcct gaacatatga atgtttcatc tttagaaaag aaactaagac
     5101 ttagtgaagc tcatataata gccagagtgt atgataataa atatgtacta gatgtaagaa
     5161 ctatatttga tgatgaattt gatgtaatag ttgatgaatt gagaaaagca tttaattaaa
     5221 taaatttgag ttaataaata ttaaatttag aagtataagt atgaagtttt gattaaaaaa
     5281 tagctacaca cacttgtgac tttagtcata ggaggtttag agggggcaaa aaatgaaaaa
     5341 tgtagtaatt ggaacagcag gtcatattga ccatggaaaa actactctta taaaagcatt
     5401 gactggaaga gagactgata cattagatga agaaaaaaag agaggtattt caataaattt
     5461 aggatttact tattttgatt taccaaacaa tagaagagct ggaattgttg atgtaccagg
     5521 acacgaaaaa tttataaaaa acatgttagc tggagcatca ggaattgata tagttttact
     5581 tgttgtagca gcagatgaag gggttatgcc acagacagtt gaacatttag atattttaag
     5641 ttttttaaat atcaaaaatg gaatagtagt tctaactaaa tctgatactg tagatgatga
     5701 atttagagag ctagtaaaag aagatatttg tgaaaaaata aaaggaactt ttcttgaaga
     5761 tgctgaaata attgaagttg attctatatc caaaagaggt atagacaccc ttattgaaaa
     5821 aatagataaa atgagtgatg atatagaaga taaaaatgaa agctctccag caagacttaa
     5881 tatagacaga gtattttcaa taaaaggatt tgggactgta ataacaggta ctttaataga
     5941 aggaaagata agtatagaag atgatttggt tatatatcca caggtattaa aaacaaaaat
     6001 aagaagcata caagttcatg gagaaaataa agatacagct tatgcaggtc aaagaacagc
     6061 aatcaatata tcaaatgtaa aagtggatga tataaaaaga ggggatgttt tagctgcacc
     6121 aaattcatta gaggaatcaa tgatgttaga tgtaaaatta tcattggtaa atcatttaga
     6181 taaaggatta aaacactggg atagattaag attgtaccat ggaaccagag agatactttg
     6241 tagagcagtt ccactagata aagaattaat tgaaaatgga gaaagtggct atgttcagtt
     6301 gagacttgaa gaaagcatag tatctaagaa aggcgataca tttgtagtga gacgttattc
     6361 tccaatggaa actataggcg gaggtgtaat aattgaccca tctcctaaaa aacataagaa
     6421 atttgatgaa aaagttatag aagcattaaa aataaaagaa aaaggtgaac taaaagatat
     6481 aatagaagag tacttaaaaa ggaatctaaa aaattatcca aatataaaag aaataatgag
     6541 ctatagtggt gctcatgaag aagatgtaaa aaaagcgctt gaaacactta tatcaggaga
     6601 taaagtattt attataggaa atatgtatat gcatgtaaat caatacaata aattaaaaga
     6661 aaatacaata aagcttttaa gtgagtacca taagaaatat agacttagaa aagggatgtt
     6721 aaaagaagaa gtaaggtcta agatagagag taattttaag actagggaga tggatatttt
     6781 attagaaaaa ttatctacag aaaatgctat aaaaatagaa aataatatag tatctttatt
     6841 agattttgag gttattttaa atgagaagca aaaagaaata gcaaaaaaaa tagaaaaaag
     6901 attaaaatca tgtggagttt cttctatact gacaatagat gaagtttctg aaggaaatca
     6961 taactatgct gaagtattgg agtcaatgat aggtaataaa gtagagaaat tagatgattt
     7021 gtatataatg gacaaagata tttatgaaaa tgcaaagaat actttgataa attatataaa
     7081 agaaaataaa gaaataacat taggagaata cagagatttg attgattcta gcagaaaaaa
     7141 ttgcatgatt atattggaaa acttcgatag aaacaaaata actaagagag tagaaaacaa
     7201 aagaattttg ttttagcaga aaatgtctgt ttttgcttta aggtataaag aaggaattat
     7261 aatgtcgagt atcgtataat taattagtat aaaatattaa ttaagaggaa gtgtaaagta
     7321 ttgaagagca taatacctga tgtaaaacca gatataaaag aaagagaaga catgagcctt
     7381 ttatttataa taatgggctc agtactttta ttatttatta taaaagatac agataaatat
     7441 gaaactttat tacataccat atatactata ttcttagtag ctacaggagt aattgtattt
     7501 aatactacaa aatttaaagt agattcattt tctacatttt taggattaat ttttatagct
     7561 actggagcat tggagtgtat atatttattc aattcattag aaattaaaac tcagtttgtg
     7621 agagaactta acattacaat atcagcaata acagatttat ttcctatatt gggagtatac
     7681 ttaagtttca aatttgtaaa agataataaa caaatttatt caagtataat tttatttata
     7741 gtaactgtta cattgacaat aatatcttta ttcataatat ctggaatttg tgaatattta
     7801 ggaaatggta cttcacaata tgctttagaa gttataatat ctatatttat aataattatt
     7861 tctattgtta gtggaattga attaaactta agttcaagtg aaggtaaatg ggaatataat
     7921 gaaaaaaaga tttttaacag aataatagta ataataatct tatcaagagt tccaaatcta
     7981 ttacatatag ttattgatga taggaatata gagagaatat tatcacatat aataataaat
     8041 gtagctatat attatttata taattacata gtatctaaga atattaagaa aacagcatta
     8101 gaattacatg acataaatga agagttaaca ctaaaaacgg aaagtttgaa agaaaaaaac
     8161 aaaaagttaa tttatgagac agaaaagata gaggaactta agggaatact tataaaaaga
     8221 gaatctagac tgcaatctac attagatgta gcagtaaata gtatagttgt atttagtaaa
     8281 gatggagaaa ttacatatgc aaataaaagc tttagaaata cttttggaga ctataaagaa
     8341 caagataaat tgactgataa aataaaaaac ttcaatgatt tgatagaaag cattaattat
     8401 gtgtttgtct atgagaagaa tgtagaaaaa cttattagta caagcgataa taaagtctat
     8461 caagctatat ttgcaccttt aattattgct tcacaaacag aaggagtatt gtgtgttctg
     8521 gaagataaga ctaagaaaaa agagtatgaa cgcaaactta ttgaagcaaa taagagaagt
     8581 agagagtttt tagaaagtat tggagatggc atagtagtgc ttgaaggtaa taataaaata
     8641 tatgttaata atgcatgtag ggaaattttt aaaaataatt tagaaagtat tgatttttca
     8701 ttagtatgta aaagtgaaga aagtatagaa aaaagatatg taatagatgg tgaagtaaag
     8761 tatgtagaga tgagtttttc tcaatatact aatgctggta ctgataaaac tattattgta
     8821 atacgtgata caacctctcg aaaaatatcg caaataaaac ttgaggaaag tcaatcatca
     8881 tattcaagat ttatagatat attacctgat ggcatatgct tattaaaaga agatttaagt
     8941 ataaactatg caaataagtc gtttttagat atgctagctt ttacaaacat agatgatata
     9001 aaagattcta atattaagct attaatgaat gcgaatactg aagaaaaaat gaaatttact
     9061 gataatatgg ctaaagtttt aaatgaaaat agaagtatac ttttactaga gtatgaattg
     9121 ataaatagtt ataatgatat agtagaagta gaattaagtg cattaccttt tgctatatac
     9181 aatactagat atataatgct tattataaaa gacttagtac ataagaaata ttccgagcaa
     9241 gcggaaaaag aactacttga aagatttaaa acagataaaa tcaaaacgga gttttttgca
     9301 aatatgtctc acgagttaaa aactccacta aatgttatat ctagttcgaa tcaactagta
     9361 gattctttct atagaagtga aaagataaaa gattacaata ataatattaa atctcatgtg
     9421 gatttagtta gacaaagttc ttataggctt caaagattaa taaataatat tatagattta
     9481 acaaagatgg agtctggatt ctatacgtta aaacttgcta aatacaatat tgtatctgta
     9541 atagaagact tatttatgaa tatagaagag tacgcactta gaaaagatat aaaaattctt
     9601 tttgatacag aacttgaaga aattaatatt tatatagaca aggttgaaat tgagagaatt
     9661 atgttgaatc tattatcaaa ctgtattaag tttactgata atggaggctg gatatatgta
     9721 agtattcatt ataaaataga taaagtaatc ataagtgtaa aagatactgg agttggaata
     9781 ccacaagata aattagaact gatttttgaa gaattttcac aagttgataa gactttatct
     9841 agaaatactg aaggaagtgg aatagggctt gctatagtta agaatctagt aagtcttcat
     9901 ggtggagata tagaggtaat aagtgaagtg aacaaaggca cagagttctt aatttcactt
     9961 ccaatgaagg gatttagtag tgaacattac acagaagata agagaatata taatattcaa
    10021 gaaaaaataa aaatagaatt ttctgatata tactattaga agtgattatg agtaaaaata
    10081 aaataaaaat actatctatt taaatattta agctataaga aataggctat cataagataa
    10141 attattatga tagcttattt ttagttaggc tttatatcta tatgtgagaa catataatct
    10201 ctaaattacc ttcaaaacca aattctccaa agttgtttgg tagtataaaa tgcataccct
    10261 tttttaaatt gtattctaca gcattgtgta taagttttcc atttccatcc aatacactgt
    10321 ataaagaaaa aggtgtcttg tgtgtaaaag tacactttcc aaaaatatct aatttataaa
    10381 cgctaaaaaa ttcatttgat acaaatgttg tgcatctaaa gtctgatgtt gacacaattt
    10441 tatagtcagt atcaaaattt atatgaggaa cattggtaac atctattgat ttttgaacgt
    10501 gaagttcacg tttattacct aaattgtcag ttctatcata atcataaact ctataagtag
    10561 tatctgaatt ttgttgagtt tctaaaatta atgttccttt acatatagca tgtatagtac
    10621 cacttggaac atagaaaaag tctccctttt ttatattaac ctttctaagt agtttgtccc
    10681 attcattgtt atttaccata tccacaaatt ctttatgaga ttttgcatta tgacctatta
    10741 taatttctgc atcatcagaa caatctatta cataccaaca ctctgtctta cctaactctc
    10801 cattttcatt ttttttagca tattcatcat ttggatgaac ttgaactgat aaattatcat
    10861 ttgcatccaa tatttttgta agtaaaggaa atttatctcc aggtgtgttt ccaaataatt
    10921 ctctattttt atcccacaat tcagatagtt ttttaccttt atagattcca ttttctatta
    10981 agcaatctcc atttttatga gaacttatag cccaacattc acctgttgtt ggcgaatcta
    11041 tttcataatt aaatttatct ttaagtgcag taccacccca tattctatcc ataaaaatag
    11101 gttttaaaaa taatggttcc ataatcctgt tcctcctatg atattaaatt tatcctacaa
    11161 ataattatat ataaatacat tataaaatca atagtaaaaa agttttgtcc cttgaaatga
    11221 cataaagtat aaggagttat taaataagat aaactatgat atataatata aagttctaaa
    11281 ttggagtgat aattatggaa ttgtttaagg aagaaatcaa aaaatacgaa aaagaatttt
    11341 tgaaattatt ggaagaatgg gtgagcatac cttcatttta tgatagaaaa actgtatcta
    11401 aagatatgcc ttttggaaaa ggggtatatg atgcattaaa ctggtttgaa aatctaggta
    11461 gagaaaacaa tttcaaagtg aaaaatatag ataatcatgc tgttcaaatt gaatatggaa
    11521 atggcaaaga gtatgtagat atatttggac attgtgatgt tgtaaatcca ggtgaaggat
    11581 gggatagtga accttttaaa ttaaatatta taggtgataa attagttgct agaggtgtat
    11641 ctgataataa aggacctatg atagtgaatt ttttagcact aaaaatgata aaggatttag
    11701 atattaattt aaaaaggaag gttagattaa tagctggagg aaatgaagag agtggattta
    11761 agtgtataaa gcattattat agcaaagagc catatggggt atgtggattt actccagatg
    11821 ctaaatttcc tgtattaaat ggggaaaaag gtggagcaat aataaagttg ctttcaaata
    11881 tagacgataa atctttatac ataagtggtg gaatagagtt taatacaata ccagataaag
    11941 tttatataaa gaatatagaa aaattgggta aagataatat atgttttgat attaacaaga
    12001 tatcgataag ttatgataat gacaattata ttgtgcatgg aaaaggtgga cattcatcta
    12061 aacccgaaaa atcaataaat cctatattag ctacaattaa acttttgtca gaaaatattg
    12121 atgaaaaatg gacaaaagat ttgtataagt tgataaatca agataatata aatggaaatt
    12181 tatttggttt aaatattgaa ggaaaatgtg ggattttatc aatggtgcca acaataataa
    12241 atatagtaga cggaaaatta gaagtagttt taagcgctag atatccagaa atattaagca
    12301 ttgaagagat aatgaaaaaa tttaatttgt atatggagaa aaataatatt aataaatttg
    12361 agcttattgg ggaaaattta aagcaagcaa attatataga taaaaattca aaactcgtaa
    12421 aaagtttgca tgatatatat ataaagtatt ctggagattt aaaaaatgat gttagagtaa
    12481 ctagtgcagg aagttatgct tctgaaatga ataattcagt aatatttggt tgtgaatttc
    12541 ctgatggtag ctttggtaat gtccatggtg cgaatgaatt tgctagctta gatagattta
    12601 taactgcaat aggtatatat gcagaggcaa taattacatt atgcaacaaa atataaatac
    12661 aaaatgtccc ttatatagac atattatata tacaattaaa attttacttg atatataatt
    12721 gaaatcaaag agaaggtgat acaatgataa ctaagaaaca tgcaataata gtaaagagtt
    12781 taagcaataa agatggatat atgacatcaa atgaattggc tatcaaattg gatgtgtcta
    12841 ctaaaactat taaaagatat atagcagatt taaatagtgt tttaagcaag tatgatttgg
    12901 taatagattc ttctagaggt ataggatata agttaagcgg ttctaaaaac aatatagcca
    12961 gagctatcaa agaagcaaat aaatatattg atggtttttt agatgattct gaagagtcaa
    13021 gaatgtcaaa tataatatgc atgcttatta atagaaatta tatgagtata gaagcaatgg
    13081 cagaagaatt aaatctaagt atagccgcta taaataaatt gtctagtaag cttaaaagaa
    13141 aacttgaaaa gtatgactta gttataaaat ctaaacctta ctatggaagc tatatacatg
    13201 gtgaagaaat taatataagg caattaatta ctgactatgc aataaaatta gatgaaaaaa
    13261 gtaaagtgaa ggtgttttta gatgacatta gtgaaaacga tatatactgt atagaaagta
    13321 ttttagaaaa acacttaaga gaaaaagata caataatttc agataaagat tttaatcttt
    13381 tgctttcaaa aataatcgtt tctgtattta ggagtaagag aggtcatagt aacaatataa
    13441 acttaatgga tacaagttat agatttcata actactactt tatagaaagt ctaatgaaag
    13501 aaatatctga taaaattgga tttaaactta ttgaagatga agtaatttat atttcaaatt
    13561 attctggtgt aatagcctgt aaaggtacac aaggaataaa aaacacaagt gagcttgagg
    13621 agcgaataaa tagtgttatg agcagtgctc ttcaagatat attccttatt tcgggcagtg
    13681 attatacaaa agatgatgag tttatggttg caatatctga ccatataaaa agatttttaa
    13741 atagggctag agctaatgta aaatcaaata atcctttact tcatcaaata aaagagaagt
    13801 ttcctatagc ttttaattta gccgtgtttt tatcaaataa actagaaact gagtttaatt
    13861 taaaacttga tgaagatgag cttggatata tagctattca ctttgctgct tcaaatgaaa
    13921 gaatgaaaaa aaatactagt aagaaaattt gtatagtttg tcattatggt ataggaacag
    13981 gacaacttat ttctgaaaag ctaaaacaga atataagtga tttaagtgtt gtaggtgttt
    14041 atccagttag atatctcgat atggctataa gtcaagatgt cgatttaata gtatctactg
    14101 tcgaattaaa aggatatgaa aaacctgtct tatatataga aaatatattt gatgatagct
    14161 taattgaaaa cgtaaacaaa gctttttatg aaaaggaaga aagaagaaag ataataagta
    14221 acatgtttga tgaaaaagct tttttcaata taaaggctaa tactaaagaa gaggtcatac
    14281 tttcactttc aaataaacta aaagaaagag actttataga ggaaagttct ataaaaagca
    14341 taatagatag agaaaatatt tcatcaactg aaataggaaa tctagtagca atacctcaca
    14401 caatagtgaa gggagataaa aaatctataa taggagttgg aatacttgaa aatccaataa
    14461 tttgggataa gcaagaggtt cagctagtat ttatggtatt ttttaatact aaagaaaagc
    14521 ataatttctc gatatttaaa tacctttata attttataaa agatgaagga ggagtaagag
    14581 ggattattaa aatatgtgac tttaataaat taatggcact gataggtaat taaggaggaa
    14641 attaataatg ggaacaaaca tatttaacaa agaatatgta tttttaaatg ttgatgctaa
    14701 gtcaaaggtt gaagttttaa aatttatatc aaaaaaagct aaagaattaa atttggcaga
    14761 agatgaaagc ttagtatatg aaggtttaat gtctagagaa gaacaattta ctacaaattt
    14821 aggagaatct atagcaattc ctcatactaa aaatgatgca attgaaaatc cagcagtagt
    14881 agtgttgaag tttaatgaag atgttgtttg gaatgaaggt gaggataagg ttaaattagc
    14941 aataagctta cttatgccag gaaaaagtaa agaaaatatt catcttaaat tgttatcatc
    15001 tctttctaga aaattaataa ataaagactt taaagattct cttttaaaga gtgataatgt
    15061 tgaagaaata tctaacttaa taaatgaagc tttaggttta taattaaaca ctaaggttat
    15121 aaaataaaaa ataattaact aaaaatagga ggaatttaaa atgaataaaa aattagtagc
    15181 attatgtgct tgcccaatgg gattagctca cactttcatg gcagcagaag caatagagca
    15241 agcagcaaag gctttaggtt atgaagcaaa agttgaaact caaggtgcag atggtgtaca
    15301 aaatgaatta acaagagatg atatattagg agcaactatg ataatacatg cagtagcaat
    15361 aactccagaa ggtatggaaa gatttgatgg gtgtgaagtt tacgaggttg agttacaaga
    15421 agctataaaa aatgcagagg gtgtcataaa agaaatagaa gaagatttag gaatataata
    15481 aatttattgt ggaggaaata gaatggcaat taagaaaaaa gttataaaca gttcaggtgc
    15541 ttctacaaca ggaggaaata atgtaggaaa atctactcca tctagtccaa ctcaatcaaa
    15601 gggaaatgga aaatggaaag aaataagtaa acatataatg acaggtatat catatatgat
    15661 accagtttta gtaatgggtg gacttatagg agctttatct caactaatac catatgctat
    15721 attaggactt gacccatctg taggtatagt tgatgctatg aactcaggag aatttacagg
    15781 gttcaagctt agtcttttaa atatagcaca attaatgtca aatttcggat ttactttgtt
    15841 tggatttgca ataccactat ttgcagcatt ttgtgcaaac tctataggtg gaaaaacagc
    15901 cttaattgca ggatttatag gtggatatat agctaataaa cctgtaggtg taccacaatt
    15961 tgttgatgga caatggactg aagttgttcc agttgcatca ggatttttag gcgcaatatt
    16021 aatagcattc ataataggat attttgtaaa atggttaaat aaatctataa aagtttctca
    16081 taactggtta gcttttaaaa caacattttt aataccacta atagcgtcat tagcttgtat
    16141 ggtattgatg atatttataa taactccatt tggtggacta attaatgaaa gtatgaaaaa
    16201 cttcttaaca gcagcaggag cagcaggaga atatgtatat gcaacagctt tggcagcagc
    16261 tacagcattt gacttaggag gaccaataaa taaagcagca ggattcgttg cacttggttt
    16321 aactacagag aatgtacttc caataacagc aagaacaata gctatagttg ttcctccaat
    16381 tggattagga ttaacaacat tattagataa aaaactagta ggaagaagag tatatgatag
    16441 acaattctat caagcaggaa aaacttctat attcttatca tttatgggta tatctgaagg
    16501 agcaatacca tttgcacttg aaagaccagg ctttgttata cctttaaaca tagtaggaag
    16561 tgtaatagga gctattacag gtataattct aggagcaatt caatggttcc cagagtcagc
    16621 agtatgggca tggcctctag tagacaactt atttggatat ataataggta tagcagttgg
    16681 tgctatattt atagcagtag gaaatatatt ctatagaaat aaattaataa aagatggtaa
    16741 acttgttgta gattatatcg actaatattt aatttttact atctataaat aaccttggct
    16801 ggtaatatgg ttttaatcaa tattaccagc cttacctata tgattaatta gaggcggtgg
    16861 agataagata tggataagat agaaaaagta agagagtatt ttagagaata tgatatagat
    16921 ggatttctta tcaatagtcc tacaaataag ttttatgttg gaaatttatt tagttcttca
    16981 ggttatgttt ttataacgaa agaagcacaa tatataatag tagattttag atattttgaa
    17041 gaaattaaaa gaaaatcgag cttgtttaat gtagtattaa tggacaaaac tagaactcat
    17101 tttgatataa ttaatgatat ttgtagagaa cagaatataa aggaaattgg atttgaggga
    17161 aatgaagtat cttttgattt atatagaagt atgagtaata agttaagtgc aaccttaaaa
    17221 tcagttgatt tatctacatt aagggaaaca aaaaatgaag acgaaataaa atatataaaa
    17281 aaagcttgtg aaatagttga tgctacattt tatcatataa tagactttat aaaggttggt
    17341 atgactgaaa agcaagttga aaatgaaatt gttagagtta taaaagattt aggtggtcaa
    17401 aaagaatctt tcgatacaat agttgcatct gggcttagag gagcattgcc tcatggaaaa
    17461 gcaagtgaaa aagttataga gtatggagat tttgttactt ttgactttgg tgctaagtac
    17521 aataattatt gttcagatat tactagaacc atttgtatgg gtactataaa taaagagtta
    17581 gaagaaatat ataatatagt aagaaaagct aatgaagagt gtataagggt tttaagacca
    17641 ggaatgacta ctggagagat agataaagtt gcaagagata taattggttc atatggatat
    17701 gccaataatt ttggtcataa tctgggtcat ggtgtaggaa ttatggttca tgaatatcct
    17761 gcattagctc ctgaatcaaa tgaagtatta aaagaaggaa tgattgtaac tatagagcct
    17821 ggtatatatg ttccaagtct tggaggcgta agaatagaag atgatgtact tataacaaaa
    17881 gatggatgta tgagattgac tacatctact aaggatttaa tagttgtcag ttaaaaataa
    17941 attaggtaaa tattttataa caataagaaa agaagaggta aatttaaatg tataattttg
    18001 ataataaacc agatagagta agtgagaagt gtagaaaatg ggatttaaat attataagag
    18061 ataaatttgg agatattaga gaagattttg taccaatgtg gattgctgat atggatttta
    18121 aaataccaac agaaatagaa aacaaattta ttgaagctgt aagaagaggt gtatttggat
    18181 atacatactg ttatgatgaa ttttatgatt cagttattgg gtggcaaaaa aatatgcatg
    18241 aggtagatgt agaaaaggaa tggattacat taacttatgg aactgtatcg acacttcatt
    18301 atgtagtgca agctttttgt aaaaaaggcg atagcataat attaaacaca ccagtttatg
    18361 acccttttga atcatcagca aaaaaacagg gtgtaaatgt aatttgtaat acattagatg
    18421 ttgtaaatga tagatattat ataaattttg ataaattaga agcacaaatt aaggaaaata
    18481 agcctaaatt aatgatgttt tgtacaccac ataatccatc tggaaggata tggacgattg
    18541 aagaaatgac aaaagtagca actatatgta aagaaaataa tgttattttg gttgcagatg
    18601 aagtacatgc agaacatatt cattatggta aatttaattc aatacttaag attgaaaaag
    18661 agcttttaga aaatgttatt ctattgacat caccaaataa aggatttaat cttggtggat
    18721 taaagacctc ttattcaata gttataaata agaatataag agacaaattt agaaataaac
    18781 taaagcaaaa ttctataact tctccaaatg tgtttggaat aatagggttg ataactgctt
    18841 ataatgaatg tcatgagtgg ttaaatggtg taaatgaata tataaagtca aattatgaac
    18901 ttttagaaac ttgggtaaat aaatatagta agataaaatt gatgaaaatg gaatcatctt
    18961 acttagcttg gatggatata agtggattag gcataagtgc aagtgagttt acagataggt
    19021 tggctataga tacaggagta ttattagaag atggaagtca ttttgtacaa gatggtgata
    19081 agtatgttag aataaattta ggaactcaaa aagaaaatgt aattgaagct ttaaatagaa
    19141 tggatttgtt tttaaaatct ttataaaaat ttggttaaat tatttattga aaatataaaa
    19201 aagtagctta ttcaattata aaaataaata tatttaatgg taggcatttc ttatgaaatg
    19261 tctattttta tatgattatt gtaaagtttc ctttacaata aaatagtaat atgatatatt
    19321 atatgtgtaa aggaaacttt acaatgaagg aggagatatt tttgaaaaat tgtgtagaag
    19381 ctttgagaaa gaaaaaaggt cttaatcaag aggactttgc aaaaattctt aaggtttcaa
    19441 ggcaaacgat tagttctatt gaaaatggaa aatataatcc atctttagaa cttgcatttc
    19501 agatatctga tttttttgaa atgtcgattg aagaaatctt tttatggaag gagatataat
    19561 ttatggaaaa aaggaattta agaataggaa tagtatatgt agttttagga gttactttct
    19621 tgttttcttg tttaatgact aaggataagt taagttcttt gcttgttggt ttagcagcag
    19681 gatttggatt taatggaatt tctataattt atcgatattt ttactggaat aagcataagg
    19741 aagaatatag agaaaagcta gaagaagaaa aaataaacct tcaggatgaa aggaatgtaa
    19801 tgtatagaga taaagcagga cgatatgctt atattgtgtg tatgattata attcctatat
    19861 ctgcttttgt tttcgctgta ctaaacgcct tagatatata caactcatta gtaatcatta
    19921 tatatctatc tgttttatgg attgttttgt atgttgtagg agttatttac tatcgaaagt
    19981 taaaaaataa tgaatagtat ttatataaat ttaagctatt aatgtggtat atacataagt
    20041 acaatttgtt ttagactact taagtatata ccacatttta tataaatttt aaatttgcat
    20101 tagttattta ataactattt aaattcatat gtattttgta tttataatag gtaaagttat
    20161 tatttaaact tattatttat ttaatttttc aatagaaaag gctttatctg taagtttaaa
    20221 attatctttt atacccttag ttaagtatac tttcttatcc ttagttatga atatagcatc
    20281 tacattatca attctattaa ttaaatccat acctttttca agtccaagtc caaatgtaga
    20341 agtggataag gcatcacaat taatagattt atcagagatt attgtaacac tacttaaatt
    20401 gttttcaaaa ggataccctg taaatggatt taacatatga tgatatattt ttccgtcttt
    20461 ttcgataaat ctttcataaa ttccagaagt aacaacagat ttatttgttg tttctatgtt
    20521 tcctatagat tcaccatttt tactagttgg gtcttgaata cctattttaa aaggtttatc
    20581 atttttactg tcttctaaag tgaatatatt tcctcctaag tttactaaac ctttttttat
    20641 atcctctgat tttaaatagg atacaatttt atcagcagca tatcccttag ctatggcacc
    20701 taaatcaatt ttcatatttt ctttgtttaa ttttatagat ttattttttt catccagtat
    20761 gatgttctta taatcaataa gaggcagaag tttttgtatc tcagaatcac ttggaacttt
    20821 agctttatcc gttccaatcg cccataaatt tgataatgga ccaattgtta tatcaaaata
    20881 tccatctgat aatttggaat attgtatagc ttcttttacg acgaaaaatg tatcatcgga
    20941 aactttgaca aatttcttac cagcattatc atttatttta gaaacatcac ttcctggtat
    21001 atgagtgctc attttgttgt caatatatct taaaattgaa tcacattcat ttaagatttt
    21061 atcacttttg gattctttta cattaaatac agtaacttcg tttactgtac caaggtagta
    21121 gctagtctta ctatatttag tattctggcc tgaactccta aaaaatgaaa ataaaattaa
    21181 tgctaaaatc aatatcacaa aaataaatgt gatttttttc ttattcatgg tatattcctc
    21241 ctatgaaatt gtgttattat tttattataa cataatttca taggaggaca aaaaatgaag
    21301 aaaaaagaca ttcttcttat tttaggcatt ttggttgtta ttacagcatg ttatgggata
    21361 ataaatgtta taaattctaa aaatgctgga aatattgaga tttatgtaga taataaattg
    21421 tataaaactg tttcaataaa tgcaaaagaa gaatttaaaa tagagaatag aggggggtat
    21481 aacatagtaa agattcatga taaaggggtt gaaatagtag atgcttcatg tcctgataag
    21541 gtttgtgtac atactggatt tataaacaag ccaagtcaaa gtatagtttg tatacctaat
    21601 agagttagca taaaaataaa tacaaatgaa aagaatgata atcaagaaga tataatttct
    21661 aaataacata ataaaaataa ttgaaaggaa ttggtgatag ttatggttaa cttaggaaac
    21721 gattgggatg aattattgaa agaagaattt gaaaaagatt actacttaaa tttaagaaaa
    21781 tttcttatag aagagtataa aacacgtcaa atttttccaa atatgcataa tatatatgaa
    21841 gctttaaagc acacttcata caaagacaca aaagttttaa tattaggaca agacccttat
    21901 catggagata atcaagctca tggtttagct ttttcagttc aaccacaagt gaaaacccca
    21961 ccatctctat taaatatgta caaagaatta aaagatgatt tagggtgttt tatacctaac
    22021 aatggatatt taatgccttg ggcagaccaa ggagtgttac ttttaaatac tgctcttact
    22081 gttagagcgc atgaagctaa ttctcataag aacaaaggat gggaaatttt cactgataga
    22141 gtaatatcta ttttaagtga gcgtgaagac ccagttattt ttgtattatg gggaagtaat
    22201 gcacgtaaaa aagtggaatt gattgataca agtaaacatt atattttaga agcacctcat
    22261 ccaagcccat tgtcagcaag taaaggtttt tttggatgta aacacttctc taagataaat
    22321 gaaattttga aaaagctagg aaaagaacct attaattggc agatagaaaa catataaaag
    22381 taaaaggtaa caaaatgata acaaaacaca ataaacaatg acattgttgt aactaataga
    22441 aatatgctta aatagttgaa aaaactatat atgcatattt atattttttt gtcaagttga
    22501 caactcttga aaaaatagtt acaatttagt tacacaaaaa acacaaataa aaaaagaaac
    22561 ttacaaagca gaattttatg aagcaaaata tatagtgtaa aaaatcttag ggaggaaatt
    22621 ttatatgaat tttaaaaaat tgagtaatgt taaagtctcg gtaatggcag ttgttttatc
    22681 agttggaata ttatcgggat actctgcgct taataaacaa gtaacattag tagtaagggg
    22741 agaagaaaga actatatcaa cattcagttc taatgtagga gaccttttag aatctcagaa
    22801 cattagctat gatgcaaatg atatagtaac tttagataaa aatgcaaagt tatctaatgg
    22861 agataaaata gaagtaattg atgtaaagga aaaaacagtt gtagaaacta aagacattcc
    22921 atttgaagtt acagttgttg aagatggctc tttattaaag ggtgattcta aggttaaaga
    22981 agaaggaaag tctggtaaaa gtgaattaat atataaagaa acttatcaca atggaaagaa
    23041 agtagaaaaa gaatttgtga aaaaggttgt taaagctgag ccagtaaata aggttgtaag
    23101 taaagggaca aaggtagaaa tgcaagtagc ctcatcaagg ggagaaagct caagaagaat
    23161 ggcttcatca tcatctagta gcagtagtag taacaatggt aaaaatatga gagtggtagc
    23221 aactgcatat actggtcaca gtataactgc aacaggaaca aaacctaagt ggggaacaat
    23281 cgctgtagac cctaaggtta taccatatgg aactaaagtt tatatacctc aatttgatat
    23341 ggtgtttact gcagaagact gtggtggagc gataaaagga aataaaattg atatctacat
    23401 gaatgattca aaaacagttt ataactgggg aagaaaaact atagatatac gtatattaaa
    23461 atagttaaat aatttatatt tacactggaa attatatttt gattatatag agaagaaatt
    23521 ctttata
//
LOCUS       DAMJVM010000052        23241 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.053, whole genome shotgun sequence.
ACCESSION   DAMJVM010000052 DAMJVM010000000
VERSION     DAMJVM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23241
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.053"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(183..935)
                     /locus_tag="PC523_002207"
                     /pseudo
     CDS             complement(183..935)
                     /locus_tag="PC523_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
     gene            complement(1223..3610)
                     /locus_tag="PC523_002208"
     CDS             complement(1223..3610)
                     /locus_tag="PC523_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2475726.1"
                     /translation="MINTTKIAKNNLKQNKSKSILIIITIILSTLMLSSIGIYIVNAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFVKGKLPTKENEIVLDSGALKALGYKNKDLGERIKISYNDYKNDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRNMSQEDFNIYVKFNDKSNLSI
                     EQIKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGISIPIGIILGYVLANIIIK
                     SFMNIDAKSSQLPVILLVAVISFISVVLSLLKPMKVASKVSIVDAVRYSGNKISNKNK
                     RKGYKNINLNRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKNNLNILQMDNPLSKDFFNQIKNIEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLSKNLIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTKEG
                     KYQEVKDSIKKIAKSNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAISYTSGMMIGSILFGGILGYIAV
                     MVLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            complement(3603..4286)
                     /locus_tag="PC523_002209"
     CDS             complement(3603..4286)
                     /locus_tag="PC523_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2475727.1"
                     /translation="MKILYTENLSKHYGKGESLVKALDNVNLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDETFIKELLKSLGLENKRDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGK
                     VLKVGDKND"
     gene            complement(4403..5713)
                     /locus_tag="PC523_002210"
     CDS             complement(4403..5713)
                     /locus_tag="PC523_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2475728.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSILSILIVTIIFLLFEYSR
                     LYKLYEHQIKNTQNIAGILIEKYPNDEVDIVKSIYNSNYSKVPIGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKNLSKIYFIVDKMAQE
                     NYLKDTTVSEETGYLVEYNEYFKEGTFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILDAVKSVTPKAKNKSITLNLNEFDSRIVPHDKKWTEESI
                     FNVLDNAVKYTQINGEINISVEETANYFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            complement(5707..6414)
                     /locus_tag="PC523_002211"
     CDS             complement(5707..6414)
                     /locus_tag="PC523_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2475729.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFSIEEAKSIFAKE
                     EICLIISDIGLPDGSGFDFCEEVRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIVRADNKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSAPKYIK
                     TVRGVGYIWGERSIKKC"
     gene            complement(6720..8561)
                     /locus_tag="PC523_002212"
     CDS             complement(6720..8561)
                     /locus_tag="PC523_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDF2475730.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLDRFEEITIFHTNDIHGRY
                     AEGDEHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMGKGKNAVELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGRKVFDSNDIKEINGVKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVSELKEKGADVIVAICHIGTNTLSKPN
                     SIDIANKVQGIDLIIDGHSHTKFENGKQVGDTLIVSTGQYLENIGEVKLTLDTDDVGN
                     IKIENKSARLIGKEEALKYKLDSSVSKKVDEIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITIRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKIYPEKSSAFLQVGGISYYFNPKQKEGERITSIQKEGKPLDLD
                     KKYVVATNDYIASGGDEFPCFSKEPILKEFGNLESAVTEYIEYKREISKNVDGRIGIK
                     SKDELISENQESDLILSENKESINDNEENLSNNIKSSDSDKNKILDKTRTRESSNLGI
                     SKREAREKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKNKKSK"
     gene            complement(8774..9658)
                     /locus_tag="PC523_002213"
     CDS             complement(8774..9658)
                     /locus_tag="PC523_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HDF2475731.1"
                     /translation="MKVLKKITILSLIILTLVGCSNGEDKKVVEGSKKIQNSKKNEQP
                     IIVHKDANILKDRYEPPKGFKRVNVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWVQGY
                     RISVNDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            9866..10783
                     /locus_tag="PC523_002214"
     CDS             9866..10783
                     /locus_tag="PC523_002214"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDF2475732.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILVDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPIGIVDCIRSMR
                     NLVEYIPYTLKIIENPQGSFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKKYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKNSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDTP
                     SIYLENATNVFENTILGEDRLSLHLNFDE"
     gene            complement(10912..12246)
                     /locus_tag="PC523_002215"
     CDS             complement(10912..12246)
                     /locus_tag="PC523_002215"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HDF2475733.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFDDADELLARTPEKLREAGIDLNIFREVIEVDSESKKIKVKNVKTGEIYED
                     YYDKLMIATGARSIMPPIKNINLKNISTLKSLYDGEYLKKLLSNDENRRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEITDVLEEEINRHNVNLHLDEVVVELY
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSSTNIKMLKNGAIIVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITERKAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVVLGGQV
                     AGFKDAVQRANVLAAAIFGKMTTSQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            12586..13974
                     /locus_tag="PC523_002216"
     CDS             12586..13974
                     /locus_tag="PC523_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDF2475734.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVTWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKENNLGTLTFKSLGMLLATTAIAAIVGIVVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLVFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     INKEVFSKKNIS"
     gene            complement(14055..15263)
                     /locus_tag="PC523_002217"
     CDS             complement(14055..15263)
                     /locus_tag="PC523_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475735.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNICYSGKDMIQEIKNSVAE
                     KSSLKDTISSIDNKVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGIIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNEAA
                     DNFLNQLKKYDVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKENYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLNLMDGQSDKYFTYLLGNRPLVKIKNIENPE
                     GLKVLFVKDSLIVPTAAFFSSVCSNIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(15279..16697)
                     /locus_tag="PC523_002218"
     CDS             complement(15279..16697)
                     /locus_tag="PC523_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HDF2475736.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYTFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMITSIIMNYIFGLLVDKYRESKIKVRIFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELINTQLTIPNIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRAPNLKEAYNYFKAMFGG
                     NGVIWSDYTYMFLKEYWIFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            complement(16715..17830)
                     /locus_tag="PC523_002219"
     CDS             complement(16715..17830)
                     /locus_tag="PC523_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475737.1"
                     /translation="MSLNPWNIFRRDKMKTLKVFFCSAKYFILVFIFFINASFISLAT
                     GDKILDLSFKKIETDLSSKITYEDTGVKIEIDSSKRDKERYLYIYQNIKENWSMYHNF
                     CIEIQNKNKSSQRINLSIQSKNMLEFRLKEGSEIFLEDRNIIYSDKINDGCIEVPGEF
                     EGKIYVNFNNLINEKSNVVLDSNTLSNIVSWGITFIPSDEEHNIVTIKKLSLLSQEKL
                     RFLNNIKIIGDEEVQIPILGQSISQYEVLGLGSNSKIKYSLIGKQNNVNLSQNGKLTL
                     SNKAKPGQIILQVNVDDNFKFGKKITLTESWSINKKDKDGVPYTLVSPEQSPTVQDMK
                     EINFMNSIITFIRILFVSLVVVCFGIYFYWRKCSKTK"
     gene            complement(17802..20108)
                     /locus_tag="PC523_002220"
     CDS             complement(17802..20108)
                     /locus_tag="PC523_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2475738.1"
                     /translation="MKKNSKKILITLTIITNIVYILWRIFYTVPKEEGMFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDRKKVHIYICDDGNREEMHVLAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPDVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLIFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKNKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYTAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEIEFRE
                     KSGNEMYIANMKAKIVNVVELNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRVNMNFQMDIKNIGKLRIVNFNYQYVLLTF
                     ENKNIYPKEIALEINEGIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTIIVSKPGEKKEKSIDEFEPMEYI"
     gene            complement(20113..21099)
                     /locus_tag="PC523_002221"
     CDS             complement(20113..21099)
                     /locus_tag="PC523_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HDF2475739.1"
                     /translation="MMMFFLGIVLIVFYFVIRGNSDTVKMQRGINIGNALESPKDFHW
                     DVKMSNKFFDDIKDADFDTVRIPIRFSDYTSDSDDFKIDEEFFKKIDGYINYALDKDL
                     IVVLDLHHFEEIMKEPGAHKEEFLKIWEQISNRYQKYDKKLVFELLNEPKENLSSQLL
                     NEYIEEAIKIIRKTNSKRTIIVGPYNFYQIDYLNELNIPKDSNIVVSFHYYEPNDFAF
                     QGNIYHKGFEHLRNITWEGTNEQMNYLKKRFDIVENWANKNKVKIFLGEFGITKEAPE
                     ASRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGNWDRDILSALIEKR"
     gene            complement(21394..22971)
                     /locus_tag="PC523_002222"
                     /pseudo
     CDS             complement(21394..22971)
                     /locus_tag="PC523_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
     gene            complement(23089..>23241)
                     /locus_tag="PC523_002223"
     CDS             complement(23089..>23241)
                     /locus_tag="PC523_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2475740.1"
                     /translation="NKCGYKNEEVKNLNIREWICPSCNETHDRDINASINILKEGLRL
                     TTIQNK"
BASE COUNT         7625 a         4038 c         2365 g         9213 t
ORIGIN      
        1 aactttcaca cttaggttta tttcatcctt atgttttagt ttggttagct ttaggggttt
       61 ccagcaattc aagtagtatt gaataggttc tatcctatct ctacatatag gtttccctat
      121 atgctgacta atttagctac taactcatga ctaaagtcac aagtgtgcat agctattttt
      181 taatcaagcc ccattatgtc acttattggc attgtgtaag ggaagtattt tttttcataa
      241 tagtcttccc atatatttaa attacaataa aaacctatta catcagtttt tctatttttt
      301 atacaatttt ttgcatcttg acttattcca actatagtaa gacctgctgg tgcagaaata
      361 gctttttgcg aaccgcctaa tattatgtct attttagatt catctacact taatctttct
      421 cctaccatac ctgctacaga atcaactaca gttaatattc catattcctt aagtaatgga
      481 catattttgc ttacatcatt taatacacca gttggagtat cacaatgcac tactgttgca
      541 tatttaaagt tactatcctt atctaaaaat gctttcaatt catcaatatc tatactttta
      601 gtatattcac ttgaaaaaag cacatactcc ccaccataca ttgttacaaa atctttaaaa
      661 ccttctccat atataccatt atcaattaca agtactctat ctcccttttc tgtaagtgat
      721 gcacatgctg cttcaagtcc taatatccct tcaccactca atatgtaaac atcgtttttt
      781 gtgccaacta tattgccaaa cttctcgcat gcctttttat aaaattcaac aaaatcaata
      841 tctatatctg gatttgttgt tttttctgct cttgctaatc ttacattttc cctagtttca
      901 gttggtcctg gtgcataatt aatcttctca ttcatatata tcagctccta tttattttct
      961 atacgattat tatacaaaat tttcatttta tatggtacaa ttttatactt ttttagaatt
     1021 gtaccaatac attttctgac ataaataata taaatctatt atatatatta tttattgcac
     1081 aataaaatac agagcaaaca tcagtttaaa ttgtttgctc tgtaactata ctttaaccta
     1141 tttaattatg aatctattta accatctttt ttatatggtt tttgctagtt aaactaacca
     1201 taaatacata aatatttata ttttattcac tgtatcttac tctatcaata agagattctt
     1261 tattaaaact tcgtcctatc aaataagttg ttataaattg agctataaga atacatacaa
     1321 tcatcaacaa gattggtact attggcaaag aatacgttgc ataagataat ccagtcttct
     1381 ttagaaccat aacagcaata tatcccaata tacctccaaa taaaatactt cctatcatca
     1441 ttccagaagt gtaagatatt gcctctgagt taagcatttt caccaattgt ttattagtaa
     1501 gaccaatcgc ttgaagcatc ccaagctctt tctttctagt aactatgcta gttatcattg
     1561 tgtttacaag attcataaat ccaatcacac ctattatgcc tgttaatgta taacccatta
     1621 tttttataaa cgaaatagta gccttatttg attcaattct actatctata aaatctgtct
     1681 ctaaaaatcc attagattta gcaattttct ttatactatc ttttacttct tgatactttc
     1741 cttcttttgt atatattcct aaactacttg ttgaatcgta tttcaaggtt ctatctataa
     1801 aatcctttcc tataccaaaa cttggaactc ccatagctat tgcttgaact ttaaattctt
     1861 tttttatctt tttatctcca tcatataaag ttaatcttat tttatctcct gcttttactc
     1921 ccatttcttt tgctgtatca acatgagtaa ttactatttc atctccagtt tgcaatcttt
     1981 caagatttat ttttccatct attagattct tgcttaatga ttttacatca ttttcgttta
     2041 tactttgtaa aaggtcatat ttaaattcaa catcatagtc ctccatacca atttttacac
     2101 ttcttgcaga ctctattctt ttaattcctt ctatgttctt tatttgatta aaaaaatctt
     2161 tacttagagg attatccatt tgtaaaatat tcaaattatt tttaggattt ttatcactgt
     2221 tcatttcata accagataat ctaacttcta tatcataagg aaagtgttct cttgccatct
     2281 tctcagcatc aaaactttct aaagcagtcg atactgttat aaatatagtc ccactaagta
     2341 taagtgatgc caatgtcata taagtacgtt ttttatttct ttctaaattg gcatgtgata
     2401 atctatttaa atttatattt ttataacctt ttctcttatt tttattgctt attttatttc
     2461 cagaatatct tactgcatca acaatagata cttttgatgc cactttcatt ggttttaaaa
     2521 gcgataaaac aacagatata aaacttatta ctgcaaccaa taatataact ggtaattgtg
     2581 atgattttgc atctatgttc ataaatgatt ttattataat gtttgcaaga acataaccta
     2641 aaattattcc tattggtatt gatattcctg ctaatataaa tccttcttta aatactatat
     2701 tttttatttg ttttttagtt gcaccaattg ctctaagctt tccaaattct tgaacttttg
     2761 taactattga aatataaaat atattgtata ttactaaaat actagataat acaattacta
     2821 atcctacaaa aactccaccc ataatcgttt ccatatctgg ttttaaagca tttatataat
     2881 tttcatttac agcagtatta atagtatcca atcctatatc attagcaatt ttatctaatt
     2941 tttcttttat ttgctctatt gataaattac ttttatcatt aaattttaca taaatattaa
     3001 aatcttcttg tgacatattt ctagtatttc tcatatacga ttctgatata atagcataat
     3061 aatatttacc tgcttcagat atttcgctag tttttaagat tccacttata atgaactctt
     3121 tttcaatttt tttatcattt ttgtagtcat tataagatat ttttatcctt tcacctaagt
     3181 ctttattttt ataaccaagt gcttttaaag ctccactatc taacacaatt tcattctctt
     3241 ttgttggtaa ttttcctttg acaaattcaa aactatttaa tttcaaagca tcttcattca
     3301 tatatgccag agaaatattt gaatcatctt ccagtttttc aacacctact ccattcattt
     3361 caccagttaa ttcaacatct gcatgattac tcaatatatc agcttgtttt tcatccacat
     3421 ttgctaatat accttgataa ttacctgaat actttatagt attttctttt tgatatgctc
     3481 ctgcattaac aatataaatt ccaatagaag aaagcattaa ggtagaaagt attatagtga
     3541 ttattattag aatacttttt gatttatttt gctttagatt attttttgct attttagtag
     3601 tattaatcat ttttgtcacc aacctttaat accttgccat cttctatgaa tataactcta
     3661 tcagccattt gggctatact gtcatcatga gttatcataa ctagagtttg actatacttc
     3721 ttagacatag attttaatat actcataacc tcatcagatg ttttagaatc taggtttcca
     3781 gttggttcat cagcaagtat tatagctggt cttgtagcta atgctcttgc tattgctact
     3841 ctttgttgtt gaccacctga taatgtattt ggtaaaacgt cgcgtttatt ttcaagccct
     3901 aatgatttta aaagttcttt tataaaggtc tcatctactt ctctaccatc tagaccaatt
     3961 ggtaacacta cattttccca tacattaaga gatggaatta aattatagct ttgaaatata
     4021 aaccctattt ttcttcttct aaacacagca agttcttctt cttttaatgt aaatatattt
     4081 ttattatcta tatacacctt cccagatgta ggtatatcaa gacctccaat catatgcaaa
     4141 agtgtacttt ttccagaacc tgattttcct attattgcaa caaattcacc ttcatttatt
     4201 tctaagttaa cattatccaa agcttttact aagctttctc cttttccata atgctttgat
     4261 aaattttcag tatatagtat tttcataaaa ttatcctctc attctaagtt atattacccc
     4321 atattttatt aatttcttat ttctggagtt gtttatactt atataataaa tgtagaggct
     4381 tacattttcc tttcatttaa tattacatag ttgtaagaaa caaagagaac ttgctcccta
     4441 tccctacttt tgaagaaact ataatatttc caccttgttc ttccaaaatt ttcctactca
     4501 aatacaatcc taccccacta ccttctacag attcaatttc atcagcagaa cctctataaa
     4561 aacgtttaaa gatattatta aactcatttt tttcaattcc tataccatta tcctctatta
     4621 taattttaaa gtaattagca gtttcttcta cagatatatt tatttctcca tttatctgtg
     4681 tatattttac agcgttatct aatacattaa aaatactctc ttcagtccac tttttatcat
     4741 gtggtacaat tctactgtca aattcattta aatttaatgt tatgctttta ttcttagctt
     4801 taggggtgac actttttact gcatctagaa tagtattttt tatatcatta tcttcttttt
     4861 ttatgtttat catactaatt tctaaccttg atatgttcac taaagaattt attaaatttt
     4921 ctaatttatt tatagaagct ttgttggtta ataagaactc gttggcttca tcttcttcta
     4981 attcctcttc tataagtaat gtattgtata attttaagga tgctaaagga gtttttaatt
     5041 gatgtgaaat atctgttact aatgatttta cactttcttt ttctttttcc aacttaataa
     5101 actttatctt taaacttcta ttaagctcat atagacagtt atttatctta ctaaatgtac
     5161 cttctttaaa atactcatta tattctacta gataaccagt ctcttctgat acagtagtgt
     5221 ctttcaagta gttctcttgt gccatcttat ctactataaa atagatttta ctcaaatttt
     5281 tatttatata tcttataaaa taatatatta tacctataac tactataaac atcattaaaa
     5341 aaataaaaaa tgattcctta aaaaaagatt ttaagtataa gctaaaattt ttatcatttg
     5401 acattttatt ttccaatcca tagccaaatt tcttaaatgc cttttctcca ataggcacct
     5461 tactataatt agaattataa atagacttta ctatatctac ttcatcatta gggtattttt
     5521 caatcaatat tccagcgata ttttgagtat tttttatttg atgttcatac agtttatata
     5581 atctactata ttcaaaaagt aaaaatataa tggttactat tagtatagat aaaatagaat
     5641 atacaataac tcttctcatt atataatcat ctttcacact taaaccatct ttgaggttca
     5701 tattttttaa cattttttta tactcctttc tccccatata tatccaaccc ctcttactgt
     5761 ttttatgtac ttaggtgcag atggattttt ttcaaccttt tgtctaagtc ttcttatatt
     5821 tactgcaatc gtattatcat ctacaaaatt accactacta tcccacaaag attctaacag
     5881 ttgctcttta gtcaatgtct gcatagaatt ttccatcaaa tattttaaca atttagtctc
     5941 tgttttactt aaaattatct cttcttcctt attatctgct cttacaatca atttattttc
     6001 tatatagtaa aagaataaat catcacaaac caataaagta taatttttta ctgtatttac
     6061 ccttttcata agcgcattca cttttgatat taataccatc aaactaaaag gctttgtgat
     6121 ataatcatct gcacctaaat catatcctgt gacaatatct acctcttcat caagagctgt
     6181 caacattatt atgtaaacat cacttttctt tctaacctcc tcacaaaagt caaaaccact
     6241 accatcaggc agacctatat cagaaataat taagcatatc tcttcttttg cgaaaataga
     6301 ttttgcttct tctatactaa atgcactaaa aacattgtat ccttcttttt ttaatttaaa
     6361 actaattcct ctatttaaag atttgtcatc ttctaaaagt aatatattat tcatccacat
     6421 gtcctccatt atcatacgta ctagctaaat tatatcatac tagtaaaacc gtttttttat
     6481 caatttataa atatatcaga ggttgttaat atgatttcat ttacttgaat aaaaactaaa
     6541 aatactcata gtaccgtact atttagtgta attatttttc ataaaataca actttaatta
     6601 aaagtaatcc tgttaaagca taaaaacaaa ggaaattagt atttatataa ctaatttcct
     6661 ttgctgttta ctattatcta aaattaaatt actatctact aacttttgct cattaacttt
     6721 tacttggact ttttattttt taactctttt tgattaaaat tcaataagca tattaatgta
     6781 cttgaaacta taaatataat tatactattt gaaaaaccta aatctcctgt tttaggagat
     6841 ttttctctag cttctctttt agatattcct agattagaac tttctctagt ccttgtttta
     6901 tctaagattt tatttttatc actatcagaa ctttttatat tattagatag attctcttca
     6961 ttatcattga tactttcttt attttcactt aatatcaaat cactttcttg attctcactt
     7021 attaactcat ctttagattt aataccaatt cttccatcta cattttttga tatttctctt
     7081 ttgtactcta tatattcagt aactgcactc tctaagtttc caaattcctt taagattggc
     7141 tcttttgaaa aacaaggaaa ttcatctcca cctgaagcta tataatcatt cgttgcaact
     7201 acatactttt tatctaaatc taatggcttt ccttcttttt gaatacttgt tatcctttca
     7261 ccttcttttt gtttaggatt aaagtaatat gaaattcctc ctacttgtaa aaaagccgaa
     7321 cttttttctg gataaatttt tactccatgc tccaatacat ctttaatctg tgctcctgta
     7381 agttctttgg taactattgt attgctaaaa gggaatacat ctacaatatc tcttattgtt
     7441 atattgcctt tttctatcgt atctctaata ttccctccat taaacaaaga aatatcagcc
     7501 ttagttttat ctagtaaaat gtcacttatt aaattaccta aattagtttc ttttgtcctt
     7561 acattttcat atgaaccatc tagagtattt gtagttacac ctataacttt atctaatatt
     7621 ttttcttgtt gctcttttat ttcgtcaacc tttttactta cactggagtc aagcttatac
     7681 tttaatgctt cttcttttcc aatcaatcta gctgatttat tttctatttt tatatttcct
     7741 acatcatcag tatccaaggt tagttttacc tcgccaatat tttctaaata ttgccctgta
     7801 cttacaatca atgtatcacc aacttgttta ccattttcaa acttagtgtg gctgtgccca
     7861 tcaataatca aatcaattcc ttgaacctta tttgctatat ctatactatt tggcttactt
     7921 aaagtgtttg taccaatatg acatattgca actattacat ctgcaccttt ttcttttaat
     7981 tcacttactt gctctgttgc actttctata gggtttttaa actctaaccc ctccacattt
     8041 ttaaatccag ttttgctctt tgtttcctgg gttgagagtc caaaaaatcc tatttttact
     8101 ccatttattt cttttatatc attactatca aaaacttttc tcccattttc aaaaatattt
     8161 gatgataaaa attttaaagt attctctcct aaattagcac ttttagataa ctcaaacagt
     8221 ctatcttttc cataattaaa gtcatgattt ccaggagtaa tatagtcata tccagcacat
     8281 ttaatcaatt ccactgcatt tttcccttta cccatattta ctattggaag cccatgcaaa
     8341 caatctcctg catctactaa aattgagttt ggagtttctt ttttcaatgt agctagattc
     8401 cctatttgta tatgctcatc accttcagca tatctcccat gtatatcatt agtgtgaaaa
     8461 atagttattt cttcaaatct atctagccca taagaaataa aactctgact tgttgtaatt
     8521 agaattataa ttaccaaaat taaatatctt ctaatcttca atataaatta gcctcctcaa
     8581 attagtttta gctttaatgt aaaatataaa ttttacatta aactttttag ataaatatta
     8641 aactatagta aaattaatta gtataatttt taatatttat ctatcaattt aattttgtcc
     8701 atatccaatt agttttaatc taattacatt ttttcacatc tatatttttc ttttattata
     8761 taaaattatt attttaaaat cttttcaact ggtctctttt aaaagtccat tgaggtattg
     8821 tcaaatcacc ttcaaaatct aaactatacc aaggactcaa atttggattt ttacgattga
     8881 taagaagttg agtttgttgt gcaggcatat agctttgggc tatcataaat accttttctc
     8941 cagtcttctc attttctgcc atatccataa ttatacttgc atgtcctgga cttccaccaa
     9001 ctataaatac atctcctatt ttcatatctt caacttttac aggctccaat tctttttcaa
     9061 gagataacgt tcctgaatag gcaaacacta tatccataaa ctttctgaag ctctcataag
     9121 atgttgaata ttcagttgct ttataccagc ttgaaccact atcgtttact gaaattctat
     9181 atccttgtac ccattttgaa tacttcgcct taaaaccatc aacaaaatca aaacttattt
     9241 catcatatag tttattctga tacaagtatt ctgctctcat aagcataatt gcgtctgcac
     9301 attgatgtaa atctctatta ccaatatcaa catcaaatac actatcataa ataccttttg
     9361 attccttagt tcttccatca tagtacttta ctttctctcc ataatttttt agcttagtat
     9421 tcctaagaaa tgattcaaaa cttccttttt ctacattgac tcttttaaat ccttttggtg
     9481 gctcatacct gtctttcaat atatttgcat ctttatgtac tattataggc tgttcatttt
     9541 ttttagaatt ttgaattttc ttagaacctt caaccacttt tttatcttct ccgttactac
     9601 aaccaactaa agttaatata atcaatgaca aaatggtaat tttctttaat actttcatta
     9661 atttctcctc ctttaaattc tcctaaatat aatttttact ttttgctaca caaattttta
     9721 tacattgtta tataaaaact ctatttgaca atatttcttg actcaagtgc acatttattg
     9781 ttatatttaa tgttaaaata attataatag gatatcataa taaaaacaaa caacagattt
     9841 gtgttaattt tagaaagggg aaaaaatgat agatttaact ttacttggat gtggtggaaa
     9901 tgtacctatg ccaaacagat ttctatcctc tgtatttata aactataaag gtagaaaaat
     9961 attggttgac tgtggtgaag gtactcaggt atctatgaag ctaaaaaaat gtggctttaa
    10021 agatatagac ttaatctgta taacccattt acatggtgac catatatttg gacttttagg
    10081 gcttttatca acaattggaa atagtggtcg tacaagtgat ttaactatag ttggtccaat
    10141 tggaatagta gattgtatac gttctatgag aaatttagta gaatatatac catatacttt
    10201 aaaaattata gaaaatcctc aaggcagttt ttctttagat aataaagttt taaggaattt
    10261 agaaatttca acaatcagtt tagaacactc tatagagtgc ttaggataca gttttaattt
    10321 tagaagaaac cctaaatttg atatagataa ggcaactaaa aatgaagttc ctaaaatatt
    10381 atggaataag ctccaagaag gccaaaatat agttttagat tcaaaaaaat atactccaga
    10441 tatggtctta ggggaattga gaaaaggagt taagataagt cttacaaccg atacaagacc
    10501 tatagaatca ataccagatt tcataaaaaa tagtgattta tttatatgtg aagccatgta
    10561 tggtgatgac ttagatatat caaaagctgt tagaaacaaa catatgactt ttagagaagc
    10621 tgctaatctt gcaaaactag gaaatgtcaa acaactttta ctgactcatt ttagcccttc
    10681 tttagataca cctagtatat atttagaaaa tgctactaat gtttttgaaa atactattct
    10741 tggagaagat agattaagct tgcatttaaa ttttgatgaa taatttaata taatacttgc
    10801 ttttaaatat ttgaattaaa aatttaggaa tgaattttac catgacaaaa atattttagg
    10861 taaattcatt cctttaattg cttttcaatt atattagcta taatctaaac attatttaga
    10921 tacattccca gctacattta aaacatccca ggttcttgca aatggtggag cataacataa
    10981 atctaacatt ccaagttgac ttgtagtcat tttcccaaat atagcagctg ccaatacatt
    11041 tgctctttga acagcatcct taaaaccagc cacttgtcca cctaaaacaa cttttgtatc
    11101 tgcatcataa ataagcttta catatatttt atttcttcct ggataataat gagtttgatt
    11161 atagtcagaa ataaatttag atttaacatt tatacctaaa tctttagctt ttctttctgt
    11221 aattccagtt gatgctgctt ccatatccat aatttttata caacttgaag atagtgagcc
    11281 ttgataagat acttctcgtc ctgctagatt ttctcctact atcctaccca atttattagc
    11341 tccagtagct aaaggcacat atacattttc attgcttact atattcttta tagttgcaca
    11401 gtctccagca gagtatatat cctccacaga ggttctccca tattcatcaa ctattatagc
    11461 tccatttttt agcattttta tattagtact tgacaagaac tcggtgtttg gtctaacacc
    11521 agtagcaatt attactacat ctgtatctat ttctcctttg tttgttataa ctttttctac
    11581 tttatcttct ccataaagtt ctacaaccac ttcatccaag tgcaaattaa cattatgcct
    11641 atttatttct tcttctaata catccgtaat ttccttatca aatacttgtg gcaatattct
    11701 atcttctaac tgaatcacat ccacatcttt tcctaacttc ttacatgcct caactgcttc
    11761 taatcctata aaaccagctc ctattattgt tactcttcta ttttcatcat tacttaaaag
    11821 tttttttaga tattctccat catataaact ttttaatgta gaaatattct ttaaatttat
    11881 gttttttata ggaggcataa tacttcttgc tccagttgct atcatcaatt tatcatagta
    11941 atcttcatat atttcacctg tttttacgtt ttttactttt attttcttag attcactatc
    12001 gacttcaata acttctctaa atatatttaa atcaattcca gcttctctaa gtttctcagg
    12061 tgttcttgct aataattcat cagcatcatc aaaaaatcca ccaacaaagt atggtaaccc
    12121 acaagcacca aaagatacta tttcggtttt ttcataaaca acaacttcat actcaggttt
    12181 tattcttttt aattttgctg cagcactcat accagctgca acaccaccaa ttattattac
    12241 tctcatttta tctctccttt gatgtctagt taatttcttc acttatttta agctttttag
    12301 tttatatata ttctatgtaa acatttttta taataatttt tagttattta tttaacacat
    12361 ttttatttga aatcaaatct tatcaaagct attttttatt ttgttagcac aattcaaatt
    12421 ccttaaatag ttattatacc ctatttttct atatttaatc acatttttca aaattatttt
    12481 tttactctat ttaagcaaat tattgatata atattgacat ttttttaata tattgatatt
    12541 atttaaactt agaaaaattt atttttaaat aaaggagaaa aaacattgga aagttcattt
    12601 ttatcacaat ttcttatgat tacagatttt aaaacgattt ttttcataat cttacttcta
    12661 ggtacattct ttattgttag acaatttgaa aagaaaaaaa tcaaattttc attgagaaca
    12721 atatatgcta cagtactagg acttatattg ggaattataa tccaagcttt tgctggtttt
    12781 cctaatgata ccactcaagt cacttggcta caagaagtaa gcaagtggta tagtctattt
    12841 ggttctggat ttatggactt attaaaaatg ttagtagtac ctttagtttt cttatcaatt
    12901 ataaaagtaa ttataaatat gaaagagaac aatcttggaa ctctgacttt taaatcttta
    12961 ggtatgcttc ttgctaccac tgctatagct gcaattgttg gaattgtcgt tgcaaatata
    13021 atgaagttag gtgtaggagc taatatgcct actctagttg aaaatcaaga acttaaagaa
    13081 ataaactctc ttgtggatac tttaagagga ttactaccat ctaatcctgt aatggctatg
    13141 gcaaatggaa atattgtagc tacaataata tttgctacat ttatagctac ctcaataaaa
    13201 agattgagca aaaaacattt tgaaacaata aaaccattta tagattttat agaagcctct
    13261 tacaagataa ttgtaagtgt gtctatgact gttattaaat ttatgcctta tgctgtggtt
    13321 gctctacttg caaatacaat aacttcacaa ggtattagct caatagtatc tgttttacag
    13381 tttatattag ctctttatat atcagttgca atattattta tcatacattt agtttttata
    13441 actttaaatg gtctaaatcc tattacttat ataaaaaatt caactgaagc tttacttcta
    13501 gcatttactt cacgttctag tttaggaaca ttacctgtaa cgatagaaag cctcgtacaa
    13561 aaccatgggg ttgatgaagg tgtatcaagc tttacagcaa gtttaggtgc aaatatgggt
    13621 atgaatggtt gtgcgggtat atatcctgct ttaatggctg taacacttgc taatatggct
    13681 ggtgtaaaca tggatatgag tttttatgtt atgttgctaa ttgttataac aattagttcg
    13741 tttggtatag ctggtcttcc aggaactgct acaatggccg tttcagttgt tatctctgga
    13801 gttggtcttg gttcttattt tccattagtt ggtggtatac ttgcaataga ccctatatta
    13861 gatatgggac gtactatgat aaatgttaat ggtgctatgg tatcatcaat tactgtagga
    13921 aaatcacttg gaaaaataaa taaagaagtt tttagtaaga aaaatatttc ttaaaataag
    13981 tatgaaaata aatggctaac cttcaaccaa ttagccattt attttttatt ttgttagaaa
    14041 atttttttag gaatttactc acaaaatgga aaaaattctt ttgtaagatt ttggggatat
    14101 acagatataa aaacaaagtc atagtcatga gactttgcat attctagtat atctccatca
    14161 tagtatctag ggtctatcat atctatattt gaacaaacag aggaaaagaa tgctgctgta
    14221 ggtacaatta aagaatcttt aacaaataac acttttaatc cctctggatt ttctatattt
    14281 tttattttaa ctaatggtct attccccaac aaatatgtaa aatacttatc tgattgaccg
    14341 tccattaaat taaggtctgc attaaaagga tacgataata tcagactctc ttcaaatctt
    14401 ccttttagtt caaatttttg agatttagaa tctgtataat aagtataatt ggtcttaaat
    14461 ttaggataaa ttaatgtaaa atcatctata ccaccatata aaataccagt ctttcttccc
    14521 atagaaccaa gaaatgactt gggatataca attgaattat aattttcttt atctctataa
    14581 taatgatttt catctagatt catattgtat tttttattaa gttgctcaac taattctcca
    14641 aatgcccaaa aagaagtttc tactttccag tgatggtctg ttttaaaaaa taaatcatct
    14701 ttgggaatac cactctttaa gatattttct ctaaaatcaa tatagtccac atcatacttt
    14761 tttagttgat ttaaaaaatt atctgctgcc tcattgctat aagaataagg tatccctttt
    14821 ggaaattttg tcactccttt tatatactta tctggagtca ttaaataaac caatttagtg
    14881 ttttgttttt ccatttcatc agaaaacttt cttactctat ctgctatgat gccaactgga
    14941 tttggagctt tagcaaaata tgtataatgc atttttccag aagtatcttt tacaacacta
    15001 aattttgaaa actcattttt acccattaat ttttgtaaat atccataagt ttcaatgaat
    15061 gtatttttat atattacttt atcatttact ttattatcaa tagatgatat tgtatctttt
    15121 aaactacttt tttcagctac tgaatttttt atttcctgta tcatatcttt tccagaataa
    15181 caaatattaa agatagagaa agcgaataaa ataattaaaa acataattgc agttattaat
    15241 cttactttaa aaaatctatt catacttttc cctcccaact aaaaattaaa ataaataaat
    15301 ggattatagc ttccagttac taaatatgtt acagaaataa agaacaataa aatagctgat
    15361 ataggataaa atacattaaa tatcatacta tatttagctt gttcaacaac aaacttattg
    15421 attttcttcg ctataggtac actaaaaata aaagcaaata taaagaaaat ccagtattct
    15481 tttaaaaaca tatatgtata atcactccat atcactccat tgcctccaaa catggcttta
    15541 aaataattat atgcttcttt taagttaggt gctctaaaca atacccatcc aaaattaact
    15601 actaataacg cataaatatg ttttataaaa ttatgatttt caatcttttc aaacattata
    15661 aatcgctcta taattagaaa aacaaagttt aaaatccccc aaattacaaa tgtccattct
    15721 gctccatgcc atattccagt aaataaccat actataaaca tatttctaat cataatatct
    15781 ttatttgcta ctctagagcc tccaagcgga aagtatatat aactcttaaa ccacattccc
    15841 aaagagatgt gccatcttct ccaaaactca ctaattgatt ttgatatgta tggataatta
    15901 aagttctctt caaatttaaa accaaacata agacctaatc ctatagccat atctgaatag
    15961 gcagaaaaat caaagaatat ttgcagagta taggctattg aaccaagcca tgcaagtgat
    16021 gcagctattt ctccttgact attcatagtg taaatatagt ctgctactat agccatgtta
    16081 tttgaaatta acactttttt acctagacct gttataaacc tacaagtacc aatagaaaat
    16141 ttattccaag tttcttttct attgagaatt tgttctgcta cactctcata tctaactata
    16201 ggacctgcta ttaattgtgg gaaaaatgct atatataatc ctacataaaa aggattcttt
    16261 tggacttctc catgtctacg atatacatct ataacataag acattccttg aaatgtaaaa
    16321 aatgaaattc ctataggtaa aacaatatta ggtatagtta attgagtatt aataagttca
    16381 ctaatatttc ttagtgcaaa agccagatat ttaaatataa ataaaacacc tatattgtat
    16441 gcacacatca aaactaaaaa aattcttact tttattttac tttccctata cttatctact
    16501 aataaaccaa atatatagtt cattattata gaagttatca taataacaac aaatttcggc
    16561 tctccccaag cataaaaaaa taaactaaca gcaagcaata tcatgttctg tatcgttcgg
    16621 ttaaatctaa atgtataata taaaactaaa gttataggta aaaaataaaa taaaaaaatt
    16681 aaacttgaaa ataacataat tcaacctcct ctctttactt agtttttgaa cattttctcc
    16741 aatagaaata tattccaaaa cataccacta ctagagatac aaataaaatt cgaataaatg
    16801 ttatgatact attcataaag tttatttctt tcatatcttg tactgttggt gattgttcag
    16861 gagataccag tgtatatgga acaccatctt tatctttttt attaatactc caagattcag
    16921 ttaatgttat tttctttcca aatttaaaat tatcatcaac attaacttgt aaaataattt
    16981 gtccaggttt ggctttatta cttaatgtca atttgccgtt ttgagagaga tttacattat
    17041 tttgttttcc tataagagaa tacttaattt tagaattact tccaagacct agtacttcat
    17101 actgacttat gctttgaccc aatattggta tttgtacttc ttcatcacca attattttaa
    17161 tattatttaa aaatcttaat ttctcttgag acaataaaga taactttttt atagtaacaa
    17221 tattatgttc ctcatcagat ggaataaatg taattcccca actaacaata tttgaaagcg
    17281 tatttgagtc tagcacaaca ttacttttct catttataag gttattaaag ttcacataaa
    17341 ttttgccttc aaattcacct ggtacttcta tacatccatc gtttatttta tctgaatata
    17401 ttatatttct atcttccaaa aatatttcac taccttcttt taatctaaat tcaagcatat
    17461 tcttactttg tatacttaaa tttattctct gggaactttt atttttattt tgaatctcaa
    17521 tacaaaaatt atggtacata ctccaatttt cttttatatt ttggtaaata tataaataac
    17581 gttccttatc tcttttagag ctgtctattt ctatttttac acctgtatct tcatatgtaa
    17641 ttttagaact taagtcagtc tctatcttct taaaggataa atctaaaatt ttatcacctg
    17701 ttgctaaact tataaaacta gcatttataa aaaaaataaa cactaaaata aaatattttg
    17761 cagaacaaaa aaacactttt aaagtcttca ttttatctct cctaaatata ttccatgggt
    17821 tcaaactcat ctatactttt ttccttcttt tcaccaggtt ttgaaacgat aattgtctta
    17881 ttttgtaata tccaatccat catttcatct cttaaaaaga aattttgcat tatgctatca
    17941 atattattca ctttatataa gatacctctt tcatcaattt taccctcaca taaatcgcat
    18001 tctaatacaa taccttcatt aatttccagt gctatttctt taggataaat atttttattc
    18061 tcaaaagtta ataatacata ttgataatta aagtttacta ttcttaattt acctatattt
    18121 tttatgtcca tctgaaaatt catattgacc cttggacttc ttcgtgataa tgttctagtt
    18181 ttttcaatac gtttttttac attaatttgc aaatcatcaa aaaaacctag ttgattagat
    18241 attgtcattg gtaaagtagg tattctatca tgaacaatac acatccaatt atctatttca
    18301 ctatcttcaa tatgtgtaat gtaagctgca tatttccact ttgaatttag ttccacaaca
    18361 ttaacaattt ttgctttcat attggctata tacatttcat ttcccgactt ttctctaaac
    18421 tctatctcaa actcttcttc tggagaaata tattctggat tttctagcaa aaatgcaaac
    18481 ccattttctg atatatcaat cgtttttgaa gaaagaacat aagaattttg ctttaattta
    18541 aaatcaattt cagcagtata acgctcagac tttcttaact gttgtcttcc tgatataaat
    18601 agcgtagcca taacaaggtt aaaaaggttt ccaatcaacc aaaataatac aactgaatat
    18661 gtcattgaac tcattttaaa tattgctaca aacattttta aaattcctat aatagataag
    18721 accatataaa taaaatacgg tattccttgt aaaaacttat acattctatt ttcttctaac
    18781 ttattcttat ttgttactga aaatttattt ttagatattg caaatgtttc aagtattact
    18841 gctggcatta aagactgaaa cattatagtt tcatatatat tagtccatct agtattcctt
    18901 atattttgag aaaatatttt aagagataaa ctacttaaaa tatacattgg caaccaaaat
    18961 atcaatactt gaagtaatgt acattttact acaatcacat taaatacaga aaacaaaata
    19021 ggtgccatta tatatacaaa gcgttttata cttgcatacc aataagtaat tgaagaaata
    19081 taacttattt tctgccaaaa tcctaaacct ctcctaaaca aaatgtttag gcgtctacca
    19141 gtttgtatac atccacgtgc ccatctttca cgttgcttaa taagactttt taaatctgta
    19201 ggagataaac ctgaggcatg tacatctgga atagcataac atctataccc cttactttga
    19261 attaagatac ctgttgcaaa atcttctgta atagaataag tataaaatcc atcaacttct
    19321 tcgagtgctt cccttgaaat aactgtatta ctaccaccat aaattacaga atttgtccta
    19381 tttctagcca attgaatgtc tctgtaaaaa taatcttgct catttggaat ccttccctca
    19441 gagtgcagat taaattggaa caagtctgga ttataaaaac tctgtggagt ttgcacaaaa
    19501 ccaacctttt catactcttc tttttctcca tcttttttag cttgctcatt ttttaaaaaa
    19561 tatggtactg tagcaattaa aaagtcatgc attggtatca tatctgcatc aaatgtagca
    19621 atcaaaggtg agtttgtatg ttgcattgca ttatttaaat ttccagcttt tgcaccttct
    19681 ctttctgttc tagttatata atttacaccc attttttctg ctagaacatg catttcctca
    19741 cgatttccat cgtcacatat gtatatatgc acctttttcc tatctggata ctgcatatga
    19801 atacatccat ttacagtttt tctcaccaag tcaacagatt cattatatgt tgcaatgaat
    19861 acatctacat gtggataaag ttcttcactt ataattggtt tttcaggata ctcaatattt
    19921 gacataccat aataatgcac aaacatttcc atcataccca ttatctctac aaaaagaagt
    19981 attatcgcac agattaaggc aaacatccct tcttctttag gtactgtata aaaaattctc
    20041 cacaatatat atacgatatt agtaataata gttaaagtta taagtatctt ttttgaattt
    20101 ttcttcataa aatcacctct tttctattaa cgcgcttaat atatctctat cccaattccc
    20161 ttcaatttga ttgtatatac caaatccaga agcaagctcc caataagccc atgaaaagtt
    20221 tcgtttttca gcttcttctc ttacagcttt tatccaagct cttcttgaag cttctggtgc
    20281 ttcttttgtt attccaaatt cacccaaaaa tatttttacc ttatttttat ttgcccaatt
    20341 ttctacaata tcaaaccttt tttttaaata attcatttgt tcatttgttc cttcccaagt
    20401 aatatttctt aagtgctcaa atcccttatg atagatattt ccttgaaatg caaaatcatt
    20461 tggttcataa tagtgaaagg aaactactat attagaatct ttaggaatat ttaattcatt
    20521 caaataatca atttgataga aattatatgg tcccactata atagttcttt tagagttagt
    20581 ttttctaatt atctttattg cttcttcaat gtattcattt agcaattgtg aggataaatt
    20641 ttctttaggt tcatttaata attcaaatac caatttttta tcatattttt gatacctatt
    20701 agatatctgt tcccatattt ttaaaaactc ttctttatga gctcctggtt ctttcataat
    20761 ttcttcaaaa tgatgcaaat ctaatacaac tattaaatct ttatctaatg cataatttat
    20821 atatccatca atctttttaa aaaattcttc atcaatttta aaatcatcag aatctgatgt
    20881 atagtcagaa aaccttattg gtatacgtac tgtgtcaaaa tctgcatctt ttatatcgtc
    20941 aaaaaactta tttgacattt ttacatccca gtgaaaatcc tttggtgatt ctagtgcatt
    21001 acctatattt atccctcgtt gcatcttaac tgtatcacta tttcctctta ttacaaaata
    21061 aaatacaata agaactatac ctaaaaaaaa catcatcata atacctattc tttttatctt
    21121 actctccccc taacactgct atcttatata taatattatt caatccctat tataacaaat
    21181 taaattatat catgttatat acaattattg taatttacaa actttatagt gttgatagtt
    21241 ttaaaacatt aaatgcaact aaaaaacaaa aatctatctt gaaatagaaa taatcctttt
    21301 tcattattaa agtgatgaaa ctatatttga cttcacacaa aaagagtgtc tcatgaacta
    21361 aaaagttcat tttgagacac tccattatat tttttataat cgttctaaca actctagcac
    21421 atatttttta actttatcat atccacaatt atcaagtgaa tatcccagtt ctatgtcacc
    21481 aaaaccttta atttcagcat tttctttttt atttaataat tcttgcataa gctcaacttc
    21541 tatatactct cttccctttt ctttttctaa cattgtaaat aactcaaagt cctgtattcc
    21601 aaaccttaaa ttctccactc taacacttct aactggcttt aaatcttttc caggataaac
    21661 aaagaacata tctccagctt tccaaatagg aaatttgtag cttgaatctt tccaagggtc
    21721 ttctgtccat aaattatagt cccatcttaa aaaaccatca agaccaaaat agtaagtata
    21781 ccatcctatt attctatttt ctacaaaagg agaacttata aaactatttg gtttctctgg
    21841 aaaacaacat acaaaccaag tcaatattcc accattgtta ttgatttttt tcttaagatt
    21901 ttctatatct ttataatttt ggatagtaag ttttaaattt attgacatat catcaatttc
    21961 atctttagct ctatctaaaa aattttggtc atgggttgct gatttatatt taacttgatg
    22021 agttcctaca gtagaattta taaattctat acactctttg actacttctg gattatttgg
    22081 ttcgtcagct ataatcctta ctctgtccca taatccacac tctattaaat gatttaacac
    22141 taatcctata tattctttta aatcatctgt attatttata aatttgaata ctttatcact
    22201 ttcatcaaaa tatcttactc ttatagggtc cttataacct tctacaggat ttccaaactc
    22261 accagcacac caattaccta atagaccaaa taaatctatc tcctgagcca ttttatgctt
    22321 atcagctatg cttatatact tatccatact ctcaaagtta cacttaatct ttccatccaa
    22381 tccttttgat acgcttacca tgttatattc atataagttt gatggatttt tataaacttt
    22441 ataacaactt tgtcctgccc atggataatc tgaaactata actgttgcaa ctttttctcc
    22501 taaagatgct aattctttta aatagttatc aattatttca aaatgtatat cagaccataa
    22561 ctcaacttta tacattctag ctaagcaact tggatgttgc cataaatcta aaaagaactt
    22621 actttcattt aaaggcttta acacgacatt ttttactttt acaggaacat caattgtaca
    22681 aaatttttct tcatcttcat atccaaagct tttaaatata tcaataccta tattaaggtt
    22741 attttcttta aaatcttctg gtacttgacc ttcaatccaa aatgtttgag gcaaatattg
    22801 ctctactaac acatctttat cccttaaaat agcatcattt acaaatgctt ttgtatcatc
    22861 ttgaacatac cccaatatat ttatactaaa attattttct aagctattag gtacatttaa
    22921 tgctaatctt actctatttc ccagaccctt ccatgatata gtactgcttt tctgaacctc
    22981 ccatgactaa agtcacaggg ttcctgcttc atagaatttt gcatactaaa atatgtctta
    23041 ctaattctcc acaggctatc cccatagttc ctacggttct tatataagtt atttattttg
    23101 aattgttgtt agtcttaatc cttcttttaa tatatttata cttgcgttta tatctctatc
    23161 gtgagtttca ttacaactag gacaaatcca ttctcttata tttaagttct taacttcctc
    23221 gtttttatac ccacatttat t
//
LOCUS       DAMJVM010000053        22478 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.055, whole genome shotgun sequence.
ACCESSION   DAMJVM010000053 DAMJVM010000000
VERSION     DAMJVM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22478
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.055"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(53..964)
                     /locus_tag="PC523_002224"
     CDS             complement(53..964)
                     /locus_tag="PC523_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HDF2475741.1"
                     /translation="MIKKLDDILDQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKVKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGDNVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(1079..1657)
                     /locus_tag="PC523_002225"
     CDS             complement(1079..1657)
                     /locus_tag="PC523_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2475742.1"
                     /translation="MNINEFLTHVNQGKPVSSKSEIHKFMVKLSNEAMRITSELNSYY
                     HEPEEIHSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKDVAENTIVGGVPAKILRVIDKITE"
     gene            1883..2821
                     /locus_tag="PC523_002226"
     CDS             1883..2821
                     /locus_tag="PC523_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2475743.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIIELEEELG
                     VKLLVRGNRKITLTNEGKLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGGGETEA
                     MRLIARTAHNFHQKYPNVKYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYTQI
                     PATDVWGLLMRKDSTLASQNTINPKDLCNIPLIISRQTLVDKAISKWLQDSTEKLNIV
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSILLDTSELDISKLNTPEVK"
     gene            complement(2995..4137)
                     /locus_tag="PC523_002227"
     CDS             complement(2995..4137)
                     /locus_tag="PC523_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2475744.1"
                     /translation="MDFKIEKYLLRKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     AYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEEVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEIQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(4155..5909)
                     /locus_tag="PC523_002228"
     CDS             complement(4155..5909)
                     /locus_tag="PC523_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HDF2475745.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVDMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYDAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQNLDE
                     IPSPYENLNPREYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLERIKRDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKQSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREAENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            6045..6251
                     /locus_tag="PC523_002229"
     CDS             6045..6251
                     /locus_tag="PC523_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475746.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(6256..7179)
                     /locus_tag="PC523_002230"
     CDS             complement(6256..7179)
                     /locus_tag="PC523_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475747.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLIIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVTYFKKE
                     KLLFEYLLSSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKFEDYDEESILLNILNVLYDVYMEDRTVEDEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKKIDPRSLIRLEEGNTIIDPIFNQ
                     ITVISKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(7433..9133)
                     /locus_tag="PC523_002231"
     CDS             complement(7433..9133)
                     /locus_tag="PC523_002231"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDF2475748.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFAK
                     LENGDEEAKELWQWFRDESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKH
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLRLLGMHAPERM
                     "
     gene            complement(9459..9872)
                     /locus_tag="PC523_002232"
     CDS             complement(9459..9872)
                     /locus_tag="PC523_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDF2475749.1"
                     /translation="MILVSACLIGINCKYSGDNNENEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGINVKSEKDL"
     gene            complement(9940..12318)
                     /locus_tag="PC523_002233"
     CDS             complement(9940..12318)
                     /locus_tag="PC523_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDF2475750.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKAEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTTDSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKSGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIERLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTHKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            12635..14086
                     /locus_tag="PC523_002234"
     CDS             12635..14086
                     /locus_tag="PC523_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HDF2475751.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLITVNEEDGMNGADALDPSL
                     IKGQYILNLDSEEEGYLLVSCAGGKTCVVSLPVEYKKVEGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLDVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKDSKLEKICCETYERLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANIWDYVIEILKSMNQY"
     gene            complement(14290..16806)
                     /locus_tag="PC523_002235"
     CDS             complement(14290..16806)
                     /locus_tag="PC523_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2475752.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSYIFSFSINAIANDNRTIRIA
                     YPIQKGLTEIDEQGNYYGYTYEYLLEIAQYTGWNYEFVQVPGDINESITKLMKMLENG
                     EIDIMGGTLYNESLQKIYNYAGNSYGVANTVLQALYENTDIDNIMSSQGVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNEEQLEALKNGKADVLLNVSMNPIEDVRTV
                     AKFSSRPFYFITPKGSTDLTQRLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETNYIKDTEKLKVGVLLNKPPFQNIDKNTGELKGISIDLLNHICDLTGLEFELVPIKS
                     QKDLEKMTKNHEIEIVAGIIYDYEYSRKHNIIMSRPFVSTQYAMMINNKLNENSLDGK
                     CLALTRGLNYEGQFKENIVWYDTLEECIEAVNKGDVDYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYEAQSICFGIIKKDKQELLNIFNKAITSISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDRESENNNNIANEIDKIRREYNKKFFDVIKSQ
                     ENGTTEMYSIVFDDTFHWIRITSKIIYNDRKIPMYVIGKISNIDYEKEEKNRLLDKVQ
                     RDSLTNIYNAAYIRKLTAQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLVGDKILID
                     VAKVLDESFTKDDIVGRLGGDEFSVYINDIINNEELINKCNDVYNRIHLIKLPNGKSL
                     SISMGVVIAKCGQEYDELYQIADNALYDSKREGRNRFKIV"
     gene            complement(17028..17849)
                     /locus_tag="PC523_002236"
     CDS             complement(17028..17849)
                     /locus_tag="PC523_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2475753.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVVEYNRESFELEPEKLLNFIKFRRSIRQYKDIEIEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIEGLYNLALNTDDNDPVRSMYKNT
                     FKKMYKRYKENGKDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVI"
     gene            complement(18383..18859)
                     /locus_tag="PC523_002237"
     CDS             complement(18383..18859)
                     /locus_tag="PC523_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HDF2475754.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYNNGKLKDYNVSFLD"
     gene            complement(18893..19471)
                     /locus_tag="PC523_002238"
     CDS             complement(18893..19471)
                     /locus_tag="PC523_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDF2475755.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRDLAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(19575..22130)
                     /locus_tag="PC523_002239"
     CDS             complement(19575..22130)
                     /locus_tag="PC523_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDF2475756.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELDVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNISAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDGERIKIKD
                     RKFKKNILKYQPNRVNKQKKLNTVPKIRVKVKNLEQLEAVLNYDVDLIYYEDINTLSK
                     ALQMVSSISIDSSISTDLDTSSSSSISKELMGSNPNNIDTLNNKRSNTAVTDKKLIYS
                     APRIVRNKEYKQFEILDNIKGINNVQIGNWGCMEYFKDKDLYIDCYLNAFNSESINHF
                     KEEGASTVCLSQELNLNEIKQTLKYTDVETEAVIYGYAPMMVTEYCPMGVVVRDCKKD
                     KRVAKCKESNYALRDFTDASYRITQDVFCRSTIYNSKVTCMLDNLCELDEIGIDVFRI
                     DFTVEDSEMVKKVIEAHIEVLSNNYKLGEKATDLYKKLDEIGTVAGHYYKGVE"
BASE COUNT         7057 a         4047 c         2506 g         8868 t
ORIGIN      
        1 gacattttta ctattttttc tactttaatt tatttatttc aaaatattta tcctatttca
       61 tatttttagc tacaatagca ccatatgcaa tagaatataa tttagattca tgtgaatcag
      121 ctcttgacac taaaactata ggagcttttg cacccataat tatacctgct gatttagagt
      181 ctgccatgta tgtaaaggcc ttccctattc cattaccaac ctctattgtt ggaaccaata
      241 tgatatctac atctccagat acttcacttt taaacccttt tatctcacta gcttcttttg
      301 aaacagccaa atcaaaagcc agaggacctt ctacaattac attgtcacca aattctcctt
      361 ctttgcaagc ctttgctaac atatctgcat ctacagttgc ttgcatctta ggatttactt
      421 tctctttagc tgccaaacac gcaactttta cagtttccat tcctaaagct tttgcagctt
      481 ttatagaatt ttttaatatc tttacctttt gttcataatc aggagctata ttcatacctc
      541 catctgtagt tataagtaat ttatggtatt tttctaactc atataccata acatgactta
      601 ataaactatc agttctaaga ccatagtctt tatttaaaac ttcttttaat atgacagatg
      661 tatctaataa tcctttcatt acaaagtcgg cttctttact tgatactaat ttaactgcta
      721 tttctgcaca ctctttgata tcatccttat ccactatttt gataccactt aaatcaaatc
      781 ctatttcttt tgatatttct cttattttgc cttcatttcc aaccaatata ggagtaatta
      841 tatttctttc tacagcatct tttattgcca ttaaaacttc tttatcatga gctgctgcta
      901 cagagagtac aattttctct cctcctttaa gttggtctaa aatatcatct aattttttta
      961 tcaaaagtaa cccctccctt atatatattt aactaaataa taccatattt aatatacctt
     1021 ttctcaattt atttttatgt tataaagaat ttatataact aaaagaccgt ttaatatttt
     1081 attctgtgat tttatcaata actcttaaaa tctttgcagg gacaccacct acaatagtat
     1141 tttctgcaac atcttttgtg acaactgctc cagcagctat aattgcatta tctcctatag
     1201 taacgcctgg aactacagtt acattagcac caatccatac attttttcct attgtgattg
     1261 gagaaggata agttgtacca cgtttttctg gtgcaatgcc atggttaagt gtagccaata
     1321 ccacattatg cccaatttga gtgttatctc caatctcaat accaccctgg tcttgaaaat
     1381 ggcaactgct atttataaaa acattttttc ctattgtaat gttttttcca caatctgtat
     1441 aaaatggtgg aaacataaaa aatgattcat ctactttctt tccaattaac tcagaaaata
     1501 aactatggat ttcttctggt tcatgataat aagagtttaa ctcacttgtg attctcatag
     1561 cttcattact caacttaacc ataaatttat gtatttctga tttactagag acgggctttc
     1621 cttgattgac atgtgttaaa aattcattga tattcatttt atacatccct tcaatatgcg
     1681 ctttaattta tttgtacctt ctaaataata ttttaataat aactaattta atttattttt
     1741 taggcaatat tattgctcct tttagtgtaa tatataattc tatctatatc aaatatctat
     1801 attaaatact taactatact taaaaagcat aataattgat ataatcatat aataaacaaa
     1861 ttaatattga ggagtgataa ttatggaaat aagagtattg agatattttt tagcaattgc
     1921 aagagaagaa agtatatctg gtgcagcaac ctttttacat ataacacaac caacactttc
     1981 tagacagatt atagaacttg aagaagagtt aggagttaaa ttattggtta gaggaaatag
     2041 aaaaatcacc ttaacaaacg agggtaaact tttacataaa agagcagaag aaattatatc
     2101 attagttgat aaagttgaat tagagtttga caatcccaat gaaattatta gtggtgatat
     2161 atatatagga ggtggtgaaa ctgaagctat gagacttata gctagaactg cacataattt
     2221 tcatcaaaaa tatcccaatg taaaatacca cctttttagt gggaatgctg atgatgtaac
     2281 tgaaaaattg gacaaagggc ttcttgattt tggtatttta attgaacctg caaatataca
     2341 gaagtatgat tatacacaaa ttcctgctac tgatgtatgg ggtttactaa tgcgtaaaga
     2401 tagtaccctc gcatcacaaa atacgataaa tccaaaagat ttatgcaata tacctcttat
     2461 tatttcaaga cagacactcg ttgataaagc aatttctaaa tggcttcaag acagtactga
     2521 aaaactaaac attgttgcaa catacaattt agtttataat gcttctcttt tggtagaaga
     2581 aggtctgggc tatgctctgt gtttagatag actcgtcaat actacagaaa atagtaacct
     2641 ttgttttaga cctctatctc ctcctttaga agctaaatta aatattatat ggaaaaaaca
     2701 tcaaatattt tcaaaagcag cagaaaaatt tttacttcag atacaaaaag atttttcttc
     2761 tatcctgttg gatacatctg agttggatat atctaagttg aatacacctg aagtaaaata
     2821 aataataata aaaaattaag gtgtctcaaa atgaactttt tacttcataa tggctctttg
     2881 ttgtatgaag tcaaatatat gttaatcatt tcagcaataa aaagaggctt atctctattt
     2941 tgagacagcc tcatttaacc aataaaatca aatacaacct aacaatatcc ttatttattt
     3001 atttattcca agctctctta aacaagcaat cgtaatttca tcttgtggat ttaattcata
     3061 agctctttct atatagtaag ttgcatcatc aatatcacca ttatttaaat aagccattcc
     3121 taaattgcaa agtatttctg ggtcttcctt tatcttagct gcttgttcaa aatactctat
     3181 tgctttatct agattattct tataagcctc acaaagccct aattccacta tagtatccac
     3241 ctgattaggc tttattatta atatcttctc aagatacatc ttagcctgtt ctatttcgcc
     3301 cataccctta tatccaagtg ctatcatgaa cagcaagttc caccaatcca tatgttcttc
     3361 ttctaatgga agtaactttt ctaatccttc tttaaactta ccttggaata caagtgtata
     3421 tccttcttca tattgaactt taaaatccat cttgcctaaa ttctcttgta tctcagctac
     3481 caaatcagca tctagcccta gtcttaacgc ctcttcccaa gtcaacttag tctttaaata
     3541 ttgaccttga ttataataat ggtagcctaa gtgataataa cctaatgcaa agttctcatc
     3601 aacatcaatt acctcttcaa gcttagccat tgcttcaagt aagaatgcat tcattgcctt
     3661 atcatccata tctttttgat actgttttgc tatctcttga caaacaatcg cataattata
     3721 cattgcatct aaatcatctg gatacattgt tataaatgct tttatatata ttaaagaatc
     3781 tgtatgttca ccgatttcaa agtattttct cgacatatat cccatatacg ctttcaaatc
     3841 caaatcaata tcttttgata aagcatttaa aaacttctca tactctgcat tgtttttaaa
     3901 cttactatct atcccaatta tatatatcat tgcatctgct atagacattg aatttaactt
     3961 atcttgagca gtcttttctt ttatgccttt tactaatacc tcgtttttta taggtacatc
     4021 taatccacct tttggtactt catagctttt taatttaaac tctccaccat gttttatagt
     4081 tataaaagct aattcctctg cttttcttag caaatatttt tctatcttaa aatccatttt
     4141 atcctcccaa atacttaaat ctctacttta tgagttttag atttctcaaa tattttttta
     4201 tcatcatact caaataagac tacattttct gcttctctta tttctgtatt tatatcattt
     4261 tttaaatcca caacattaaa tctaaatggc tcaaaatgaa catattttat tatgtgctta
     4321 gctggtttat caacaaaact aggtaaatat gttgataagt tatcattatc ttgtaaaaat
     4381 acatggcatc tattcttaaa atcatccaca tctagcttat taaaaaactg tggtatattt
     4441 gatgttttgc ccagtgatat atagtcatat tttaatatat cattgaatac atcattatct
     4501 atattgattt tttcattata gaaatccatc aatattttat ataattgatt ctttccttgt
     4561 gccaaatcaa agtagccatt ttcatcaaag tacttagcaa aaacttcaaa gaatttaaaa
     4621 ggactttgtt tataaaatct gcctataata tatctcatag ataacacaaa atttttagaa
     4681 ttgtagtatc tctccagtat atcttctata tcctttagct tcaaagtttc ttcatagctt
     4741 atataattat tgtataaaaa ctcatatgga gcgtaatctt tatatttgaa accatgctca
     4801 tctgcaactt ttctcatacc tgtcccttta atcatcttta aaaatcctaa ttgtagatgt
     4861 tctataccta gattaaatac atcattaaaa gaattttcaa agctcttata atcttcataa
     4921 ggcagtcctg cgattaagtc taagtgctga tgtatatttc tataagatgc tacagtctgt
     4981 acaacatggg acaacttttt aaaatcatct cttcttccta ctgcatcaag tgttttttca
     5041 ttcgttgact gaacacctat ttcaaattga aaaagacctt ctttgcaatc agccaaaaat
     5101 tcaagcatct tatcatctat caaatacgct gtaacttcaa aatgataagt tgtatatcca
     5161 ttatcatttt tcataagaaa atccataatt tccatagcca cttttctatt tgcattgaat
     5221 gtcctatcaa taaactttat ctgtgatact cttgcatcta ttaaggcttt taaatctctc
     5281 tttatcctct ctaaactaaa ataccttaga cctggaatag tagatgataa acagtactgg
     5341 cagttaaaag ggcaacctct tgatgattca taatacacta ttctattttc atattctctt
     5401 ggattcaaat tttcataagg actaggaatc tcatccaaat tttgaagtaa ttccataggc
     5461 ttattcacta ttatctcatt gccttgtcta tatacaagtc catttacatc atttatttcc
     5521 atctttcctt gcaaatgaag tactaagtct ctaaaaatta attctccctc tccataaagt
     5581 atataatcca caaaatcata ctttttcatt gcatcataac tatcataact aacttctggt
     5641 ccgcccaaag ctattttagt attaggtcta attttcttta gattatcgca aagcttaact
     5701 atgtctccca cattccagat atatgttgaa aacaatatta tatcatattc gtttttatat
     5761 atatccttca aaatatagtc caaatcgttg tttatcgtgt attctttcat atcaacttct
     5821 atcaaatctc ttacaaattc ttttaaatat cttatagcca aatttgtatg aataaactta
     5881 gaatttaatg ttgttaataa aattttcaaa tatttctcac ctctttttaa gattatactg
     5941 tctttttgta ccccttatag gttaaaatag tatatgtaaa gtctaaataa tatctatata
     6001 attaatttat taatttcaag taatgtgagg aaaggaagtt ttttatggaa ttaattgaaa
     6061 aagtatctat caactcttta tcaaaaagag atttactact tattataaag tctcttgaat
     6121 acactaatga aaatactcaa attagtgatt tcattgacct taggaataat atagtaaagg
     6181 aattatgttt tttaactgac actaaggaaa aagatttttt agattatcta gaaaaaaata
     6241 gcacactcta atcacctaaa cctttttaaa ctttaatata taaatacctg atttagaatt
     6301 tatttttatg cttaatatat tatcattaaa ggtttttgat ataactgtta tttgattaaa
     6361 tatggggtct attatagtat taccttcttc aagtcttata agacttcttg ggtctatttt
     6421 tttattgtat gccttaactc caattttata ttttctcaat ttaactacat attcagacat
     6481 agaaaaaata aaatctatat tatcttcatt taatttacta tcttcaccta ggatggataa
     6541 tatagatttt tttatgctat taattccttc atcttctaca gttctatctt ccatatatac
     6601 atcatataga acatttaaaa tatttaataa aatactttct tcatcataat cttcaaattt
     6661 caaatcaata tatttatcaa ttaattgtat tgctttaatc cttgccatct gaaactttac
     6721 aacatctgat ttgtctaatt ctatagaaaa tcctattatc ttgtctttaa tactctgtag
     6781 taattcgaca tattctattt tactattatt tataagattg tgcataatgg aattatacac
     6841 tacagaactt aataaatact caaataaaag tttttctttt ttaaaataag taacatattt
     6901 ttttattagt ttaataacct catcttcgct tttttgaata ttagtatttg ataaaattag
     6961 tggtaataaa atatttaaaa aattgatatt gcctattttt tctccataaa atctataata
     7021 aataagttca tctctcagtc tattttcgtc tatatacaca catctttgcg ttttatcatc
     7081 tattaaggat tttgtattta ttataatcaa atctttaaga gccctacttg cactactaaa
     7141 agaagatgta tagacttttt ttagtaattc gtcattcata aatatatcct ttctgaaaaa
     7201 ttgaatgtaa ataaattgtt attaaaaaaa aagagacagc ccatttaaga agctgtcata
     7261 tataggggga tagtagtgta caaaaatcat tgtaatctac tatctatata ttattatacg
     7321 atactacttt tttatatgta aagcatcagt tttcgacatc cctatatttt acagttatac
     7381 aaattgcgta ctaagttcaa ttttattctt tttatttcaa cattttgtca tgctacattc
     7441 tttccggagc atgcattccc aacaatctca gagaattttc taaagtttgc tttgttgcta
     7501 aaactaacgc aagtcttgct tttcttattt ccaaattttc tactaaaatt ggattgtcat
     7561 gataaaattt attaaacgct tgagctaaat ctagcataaa tctagtcact atatgtggct
     7621 catttttctt taaagcaagt aaaatattct tattgaatga ttctataact ctaagaactt
     7681 ctgcactatc ttcatcagct aatagactat aatctatatc agaagtcact tctacttctg
     7741 cttttcttaa aactgcacag catctagcat gtgtatactg aacatatggt ccagtctcac
     7801 catcaaaact aagcgttctt tcccaagaga aagtatagtc tttaattcta ctattagata
     7861 actcttggaa tactactgcc ccaacaccca cttgcttaga tatctcatct acgtttttgg
     7921 catttgggtt ttttgctagt ataatctcct tagtcttatc tatagcttga tttaaagcat
     7981 cttctaaaaa tacgactctt ccttttctag ttgacatagt tccttcttca agagctacca
     8041 taccaaatcc aacatgaatt aagtcttttg cccaatcata tcccataagc tcgattacct
     8101 taaaccattg ttggaaatgt aagttttgtt gagaaccaac tacatatata cacttgtcaa
     8161 aatcataagt ttcttttcta tatattgcag cagctaaatc tcttgtcata tataatgttg
     8221 aaccatcatt cttagtaata agtgctggtg gcattttata ctcttctaaa tcaacaattc
     8281 ttgctccctt agactctttt aaaagatgtt tctcttccag tagttcaata accctgttca
     8341 ttttatcaga atagaaactc tcacctgcta aagaatcaaa ttctatatct aaaagtttat
     8401 acactctgtt aaactctttt aaactctcat ctctaaacca ttgccataat tcctttgctt
     8461 cttcatcacc attttctaat ttagcaaacc atgctcttgc ttcatcttcc atttctggat
     8521 gttgttcagc ttcatcatgg aatcttacat ataattttag taattctgga attggattag
     8581 actctactac ttctttttct ccccactttt taaaggccac tattaactta ccaaactgag
     8641 ttccatagtc acctaaatga tttattctta tagtcttata tccttgtgag tcatatatct
     8701 tatataaagc attacctata actgtagttc ttatatgccc tatatggaaa ggttttgcta
     8761 tatttggaga agaatattca actattacag ttttgtcttt tccaaaatca ctatgcccgt
     8821 aattttcttt ttcatctaaa acttttttaa ttacagtttc tgctaattga gatttattta
     8881 caaagaaatt tacatatccg cctaattgta ttacttttgt tatctctcca cttggttcta
     8941 tactttccgc caattcactt gctatcatgt ttggagcttt tctaaacact ttggctaatt
     9001 taaagcatgg aaaagcatag tcccccatat ccttatttgg tggaacttct ataagtgcct
     9061 ctatctcttc tttcgataaa tcttctattt tttctttaag gcaatttgat attgccacct
     9121 tgaaatcttg cattctgttg ctcctctcta cattgttcct ggtttatatt gtgcttatct
     9181 ctttatgtta aatcaaattt cgtgtttttt caagtatttc attacagtgc aaaacacttt
     9241 aataattatg ttttctgtat atattaaaaa tactataatt acaattttta atcacacgat
     9301 tattggtttt ttataaactt ttttctatct tattgtttct tatactaccc gtttgaattt
     9361 acatactttt ttactctttt gccatttttc atacatcaat gttgacttta tatacttttt
     9421 tactctttta tcatttttta tgcatcagtg ttaatttgct ataagtcttt ttcactctta
     9481 acgtttattc cttcttcttc cagtaatctt gccgttatgc ctttgcctgg tatcttttta
     9541 cctgaaaaac taccatcata tattagatta catccacaag aggggctacc ctcttttagt
     9601 attgcctcgc tacatacata taaatttgct atttttagag tttcattagc ccctcttaca
     9661 aagttttctg ttacatcctt attatcttta gtaattactt ttccatcaac tatctcagca
     9721 ggtggtcttg gtgtagatag accacctaac tgctctgggc atacaagtgt aaattctttg
     9781 ccttttaaat attccttaac tttttcattt tcattattat cgccactata tttacaattt
     9841 attcctatta aacatgcaga taccaatatc ataaaaatgt gctcctatac aaattattaa
     9901 tatattctca tacaaattac taatgtattc ttatacaaat tattttaatt taacaattgt
     9961 tactcctgct ccaccttctc catattgacc acctctttgt gattttacat gtctatttct
    10021 tcttaaaatg tcttgtagcc ctgcttttaa tactcctgta cctattccat gtattactgt
    10081 tacagactct aaacctgcca catatgcatc atcaagatac ttttcgactt ccattatagc
    10141 ttcttctagg tttaatcctc taaggtcaac ttcatttttt acactaccag attttgacct
    10201 tataatattt ctagttgatt tggttacatt tgttgaaaca tctttgtgtg ttttttgtaa
    10261 agacttaaaa ggtagagtca tcttcattat accaatttgc actactgctt cttttctctt
    10321 tttatctact gaaactacac taccattttg atttaaagtt attaccttaa cttcttcacc
    10381 tggctttaag tcttttatct ccttatttga tacctttgga actatcatac tttttacggt
    10441 tggctgaaga cttcccatag aactagtcaa ttcttttcta agttcttcta tcttacgatt
    10501 cttttctttt gaagctcgtt cttgttcaag acttctaagt tcttttatta taatgtctac
    10561 ttcttcttta gcttgtctta taattgaaaa tgcttctgat ttagcttttt caatcatctt
    10621 atctctttga ctaaccaatt tttcaagttt ttcatcatac tctactttca gtctttcaat
    10681 ctcttctttt aatctttcag cttcttctct atcttctact gctttgattc tatttttttc
    10741 aacattctgt aacacatctt ctaaagctat gttttctgta tttatatatt ctttagcacg
    10801 tgaaatcaca taatcactta gcccaagttt tctagaaatt tcaaatgcat ttgattttcc
    10861 gggaacacct ataagtaatt tatatgtcgg gcttaaagtt tctatatcaa attcaactgc
    10921 tgcgttttct acacctgatt tagtaagagc ataattttta agctcactat agtgagtggt
    10981 agctatacat ttagctccta cagaatttat atcttctaat actgctatcg caagggcagc
    11041 tccttcaaca gggtctgttc ctgctcctaa ctcatcaaat ataactaagg agtctgttgt
    11101 tacgttttgt aatattgata ctatattcgt catatgagat gaaaatgttg atagactctg
    11161 ttcaatactc tgttcatctc ctatatctgc aaatacatta tcatatacac acatactact
    11221 tccataatca gcaggtatat gaagtccact ttgtgtcata agtgcaaata gacctacagt
    11281 ttttattgta acagtcttac cacctgtgtt tggacctgtt atcaccaatg tgtggaaatc
    11341 tcttcctaaa taaatcgtat ttgctactac tttcttttta tccaacagtg gatgtcttcc
    11401 attttttatg tttaaatatt tatcctcatt taaagttggt tctattcctc tcatctgtat
    11461 agaaagtttt ccctttgaaa atgcaaagtc caatcttcca agtatctctt gatttgagat
    11521 taaatcttca ctcacttctc caaccatagc agaaagctct gataaaattc tttctatttc
    11581 ttcttgctct cctagtttca actgtctcaa ttcattgttc atctctacta tagtcattgg
    11641 ctctataaaa agtgtcgcac cagaagatga ctggtcatgc actataccag ctacttgcga
    11701 cctatattca gcttttacag gtactacaaa tctatcacct cttagagaaa ttattgcatc
    11761 ttgtaagtat ttttgataag tagttgaact tattattgaa tttagctttg acctaatgga
    11821 ttgatttttt tgagctattc ttcttctgat atctctaagt gtacttgaag cattgtcaga
    11881 tatttctact tcactaatta tagcattgta tatctggtct tctatgtcct tatatacata
    11941 tagagaattt gatagagatt gtattattgg ataattaaaa tcttcttcat cactgctaga
    12001 taagctattt cttaatcttc tagccactct taaagtatct gctatcataa tcagacttcc
    12061 tgggtcaaga gaagcaccta tataagctct ctttaccttg tcttctatat catgaatacc
    12121 ttccaagcct actgaacccc tctttataat aattgattga gcttcactag tttcttctag
    12181 catagatttc acttctacaa aatcagtatt tggtactaaa ttttctatat attttagacc
    12241 taaggaagat gaggctttct tctttaacaa atctattatt ttattgtatt ctagaactct
    12301 taacgatttc tcattcattt tctatattca ccatcctcga tattttaact tttttattta
    12361 attttaaaaa tgtatgtttt caagcatttc caataaaaac tgcaagtttt atttcttttt
    12421 catttctaaa attattatat tttttctctt ttagatatat tttacacata ttttgtatta
    12481 tactatgtat gtagctatct atatattata cataaacatg ctttatttgt aatataatct
    12541 gttaaaaaat cttctttaat atttttaaag gagtttattt tagcttaaaa aaaaatagct
    12601 acttatatag ataaattaag gaggaacatg agtcatggga aacgtattag aaggattaaa
    12661 accagaaagt gtttttaaaa actttgagaa aatatctcaa attcctagag gttctggaaa
    12721 tgaaaagggc ataagtgatt ttttacttag ctttggtaaa aatttaggtc ttgaaactat
    12781 tcaagatgaa agcttaaaca taataataag aaagcctgct acaaaaggat atgaaaattg
    12841 tccaggtgta gttcttcaag gacatatgga tatggtttgc gaaaaagaaa agaatgttga
    12901 acatgatttt cttaaagacc ctataaaatt aagaattgat ggtgacatga tatatgctac
    12961 tggaactact ctaggagctg acaatggtat agctgttgct atgggtatgg ctatattaga
    13021 agataatact ttagagcatc ctgcactaga agttttaatt actgttaatg aagaagatgg
    13081 tatgaatggt gctgatgcat tagaccctag cttaattaaa ggtcaatata tcctaaatct
    13141 ggactccgaa gaggaaggat acttattagt tagctgtgct ggtggtaaaa cttgtgttgt
    13201 tagtttacct gttgaataca aaaaggttga aggtgacaaa caaggattat tagttgaagt
    13261 tactggatta ttaggtggac actctggtat ggaaattgtt ttacaaagag caaatgctaa
    13321 caaagcaata gctagagtat taagtgtatt agatgttgat tatgaattag cttctgttga
    13381 tggtggtact aagcacaatg ctatacctcg tgaagctaag tgtgtaatag ctgttaataa
    13441 agctgatgtt gaatctgcta aaaaacaaat aaatgacatt ttaactgcat ttaaacatga
    13501 gttcactact tctgaccctg gcatgactta ctcagtagct gaaacttctg tagacaaagt
    13561 tcttactaaa gattgtaaag aaaaggttgt tcaaatgtct tgcttaactc ctcatggagt
    13621 tcaatcagta agtcttgata tagaaggatt agttgaaagt tctactaact tcgctataat
    13681 tgaaactaaa gaatctacta ttgaattttt aacttctgtt agaagttctg ttatgtctat
    13741 tagagatgaa attgctgata gaataagact tttagctcaa gctttaggtg ctaattacga
    13801 ccttatagct caatacccag cttgggaatt taaaaaagac tcaaaattag aaaaaatatg
    13861 ctgtgaaact tatgagagat taactggtaa agttcctact gttatggctc tacatgctgg
    13921 tcttgagtgt ggattattac ttgataaact tcctcatgct gaagctatct ctataggacc
    13981 agatatgttt gatgttcata ctccaaatga acatgttagt atcccttctg tagctaatat
    14041 ttgggattat gttattgaaa tattaaaatc tatgaaccaa tattaaattt aagttttgat
    14101 tctaaataga ttttagtctt aagtaatcta tataaaatta actctgtaag ttaatactac
    14161 tgttagtata acatttactt tcaatattaa cttatagaaa tagagcctaa tgatagatag
    14221 taacatgtat cattaggctt tattgtaatt taaatattat taagctttat tctgttttaa
    14281 aattaattct tatactattt taaatctatt tcttccttct ctcttagaat cgtaaagagc
    14341 attatctgct atttgataca gttcatcata ctcttgacca cactttgcta taactacccc
    14401 catgctaata gacaaacttt ttccatttgg caatttaatg agatgaatac gattgtatac
    14461 atcattacac ttattgataa gttcttcatt atttattata tcattgatat aaacactaaa
    14521 ttcatctcca cctaaacgac ctactatatc atcttttgta aaactttcat caagtacctt
    14581 tgcaacgtca atcagtattt tatctcctac caggtgacca taagtatcat taattgactt
    14641 aaaataatca atatctataa tcaataaagc accatttttt tcagttgata aattcaataa
    14701 attttgtgca gttagttttc taatatatgc tgcattataa atattggtta aactatccct
    14761 ttgaacttta tctaataatc tattcttttc ctctttttca taatcaatat tagatatttt
    14821 cccgattaca tacattggta tttttctatc attataaatt atttttgaag taattctaat
    14881 ccaatgaaaa gtatcatcaa acacaataga atacatttca gtcgttccat tttcttgtga
    14941 ttttattaca tcaaaaaact ttttattata ttctcttctt attttatcta tttcatttgc
    15001 tatattgttg ttattttcac tttccctatc atttttatct aaaccataat aagtctcaat
    15061 acccttttct ggcatagaaa ttatcaattt atcttgctta taatcatact caaaaaaata
    15121 ttcacttgtc atttcataca actgagtatg ttttttgagt tccaaagata tcttctcgtt
    15181 catactcgta cgagtatgta aaccaaatgc cataaatcca ataatacaaa ttaacaaact
    15241 agctacaata aaaatagtct caattggatt ctgttgcata atataaagta atgaaaaatc
    15301 ttgtctatat gtagtatttt ggtagacaat tgattgcatt ttttctgatg aaatacttgt
    15361 aatagcttta ttaaatatat tcagtaattc ttgtttatct tttttaatta tcccaaaaca
    15421 aatactctgt gcttcataag tttgaggaac tagacgcaca tttttatact taggttgatt
    15481 catatagtac tgcatagaat aaccttcacc ataagtataa tctacatctc ctttattaac
    15541 agcttctata cactcttcta aagtatcata ccacacaata ttctctttaa actgaccctc
    15601 ataattaagt cctctagtta gagctagaca ttttccatca agactattct catttaattt
    15661 gttattaatc atcatagcat attgagtaga aacaaatggt cgagacataa ttatattatg
    15721 tttacgagaa tattcataat cataaattat tcctgctaca atctcaattt catgattttt
    15781 ggtcattttt tccaaatctt tttgagattt tataggtaca agttcaaatt ctagccctgt
    15841 taaatcgcat atgtgattta acaaatcaat tgatatacct tttaactcac ctgtattttt
    15901 atctatattt tgaaatggtg gtttatttaa aagtactcct acctttaact tttctgtatc
    15961 tttaatgtag ttagtttctt catcagataa aaacatctga ttattctctg gtgtaaaata
    16021 tttctcatat aaagtagttg aaaaatatgg atccttctgt tcaatattat taattgctga
    16081 attgagtctt tgagttaagt ctgtactacc tttaggagta atgaaataaa acggtcttga
    16141 tgagaatttt gctacagtac gtacatcttc tattggattc atactcacat taagaagtac
    16201 atctgcttta ccattcttta gtgcttctaa ctgttcttca tttgttttac agtataccaa
    16261 ttcaggtgac atcaaattca tcttacaata ctcatctaac tccttaagcc tagtatcaga
    16321 attttctatt acagcaatac gaattttttt tacaccttga gaactcatta tgttatcaat
    16381 atctgtattt tcatataatg cttgtagtac tgtatttgct actccataac tattccctgc
    16441 ataattatat attttttgca atgactcatt gtataatgta cctcccatta tatcaatttc
    16501 accattttct aacatcttca ttaattttgt aatagattca tttatatctc caggaacttg
    16561 aacaaattca taattccatc cagtatactg tgcaatttct agtaaatatt catatgtata
    16621 tccataatag ttaccttgct catctatttc agttagacct ttttgaatag gataagcaat
    16681 ccgaatagta cgattatcat tagctatagc attgatacta aaagaaaata tgtaagaagt
    16741 gaatatagat gtaattacaa taattaataa aagtaatttt tttaaattca ctacacaatc
    16801 ctccatatag ttaagattta cagtataaat tactgcataa acttgtagac atgaaaaaat
    16861 agattagtat ataaaaggat atataaattt actcagaaat ttaaatatcc ttaatatata
    16921 ctatatattt aaattataac tcatttatat atctattaca acaaatttac cctgctaaat
    16981 acctacccta tatcccataa ttttacaaat tgtagtgagg ctaaattcta tataacctca
    17041 aatttagcag tgtttctatt tacagttctt ttatatttca catctggata acccagtata
    17101 aatgaagtta taactgcttc attctcgctc attcctaaca attcttttat ctttggctct
    17161 acatttgatg ccataacaaa aaatccatta taacaaattc ctaatccttg agaataagcc
    17221 ataagttcca tattagatgc agctaatcct ccatctacat aagcacttcc acctagagac
    17281 atatcaccta caactaccat aagtagagga gcatcataaa ataaagaatc ctttccgttc
    17341 tctttatatc ttttgtacat tttcttaaat gtgtttttat acatagaacg tacggggtca
    17401 ttgtcatcag tatttaatgc taaattatag agaccctcta ttgctagttc ttttacttct
    17461 tttagttttt cttttactaa tatgtatctt ataggctgtc tattcccacc tgttggtgtg
    17521 tatctaccag cttccactat gttttttatt ttttcttctt ctatctctat atctttatat
    17581 tgtcttatac ttcttctaaa tttaataaaa ttcaataact tttcgggctc taattcaaaa
    17641 ctttcacgat tgtattctac tacttcattt ttatcaaatc cctgcatttc tattgcatct
    17701 gttggacaaa ttgccataca gtgaccacag ttaatacaga gaatattttt cacctttgct
    17761 ttatcatcct taacttttat tgcatttaca agacagtctg attcgcacat cccacagcct
    17821 acacattttt ctaaatttat gtctatcatt ccaaattatt cctcctttta aaaaatttac
    17881 tttagtataa aacaaatagt tgtctaagtc aatttttatt tttttaacaa tatattagtc
    17941 ttaactacac tttaattgag aaatatatat tagaacttat tcaaaatgaa agataagaaa
    18001 agtaggtgtc acaaagtgaa ctttttagtt gatgagatat tctttattgt gtgaaatcaa
    18061 ggtattttta tcatttcaac aataaaaaag gcttatctct attttgagat aacccttttt
    18121 ctcaactaat gttatttatc ttaatattat gccaatcaat gtgaactacc atccacttaa
    18181 ctacctacgg taatgtttga agtggtggct tcttgttcaa gtaactattg ttatcagtat
    18241 ctccaagcta ttaggctagt tcctagctct tatgattgcc atttatatgg ctaagtttag
    18301 tagattgata cttgcattga tatctctatc ttaaataatc taagaaacta caccttaatt
    18361 aatctaaaaa ctacaatctt aattaatcta agaaacttac attataatct tttagtttcc
    18421 cattattata tacaatcttt gcactaaaaa tgtttcctgt cttagcccaa tttaagaaca
    18481 acctgtcgcc ttcccacata tttaaatcga atattttatc attatcaacc caaattaaat
    18541 ttccttcttt acagtcattt aaatcttttt cagataattc accttcaaat tcatctgaga
    18601 aatacaaaaa catataccaa tcttcttcat ctttaaattt tggaaaagtt atcaatcctt
    18661 tatatgacaa agacttaaga gtaagaccag tctcttcttt tacttccctg attacacatt
    18721 cttctggagt ctctccttgc tcaaactttc ctccaactcc aacatactta ccttcatgta
    18781 tatcatcttt ctttttattt ctgtacaaca tcaaagtctt gtcatctttt tctatataac
    18841 aaatagttgt aagtttcata tatctcgcct tactttctct ttatattcta aactaattat
    18901 tttcagcact ttcaagcaca tcacttatat tcttcattgc attaagagaa agctctgcaa
    18961 attcatcaaa tggaatccct atatcttcta tacttctcat tgcatctcta tttacatttg
    19021 aagcaaatct cgtttctttc attcttttaa tcacagactt agtttttaca ctagtaagtt
    19081 ttttatctgg ataaactaaa gctatagcag ttataaagcc agttataggg tctgctgcat
    19141 atattgcttt ttccattagg ctagttcttg taactcccat cccatcatga tgaccaagta
    19201 ttgctctata catttcttca tcaccgaagt tttctttttt aagcagttct actgttgttg
    19261 tagcatgtcc ttcacatatt tctcctggct ttcttccaac tatatctgaa tccaaatcat
    19321 gtaaaagacc tgccactgcc cactcttctt ctttttctgg ctctaatctt ttggctaagt
    19381 ctctcatgac agcttcaaca gcataacaat gttttctcat tctatcagtt tctaaatact
    19441 taaataaaat ttctaaagct tcttctctca ttttcactaa tccccctgtt ttttcatata
    19501 tatgatattt ttatatatta atacacataa attcaagttt ttatatatat tcatgtatat
    19561 ttttcatgtt atatctattc tacaccttta tagtagtgtc ctgcaactgt tccaatttca
    19621 tccaattttt tgtacaagtc tgtagccttt tccccaagtt tataattatt actcaagacc
    19681 tcaatgtgag cttcaataac ctttttaacc atttcactat cttcaactgt aaaatctatc
    19741 ctaaatacat ctattccaat ttcatctaat tcacataaat tgtccaacat acaagtaacc
    19801 tttgaattgt aaattgtact tctacagaat acatcttgtg ttatcctata acttgcatct
    19861 gtgaagtctc ttaaagcata attactttcc ttacatttag caactctctt atccttttta
    19921 caatctctaa caactactcc cataggacag tattctgtaa ccatcatagg agcatatcca
    19981 tatattactg cttcagtttc tacatctgta tattttaaag tttgtttaat ttcatttaag
    20041 tttagttctt gtgacaaaca aactgtactt gctccctctt ctttaaagtg atttatactt
    20101 tcactattaa atgcatttaa gtagcaatct atatataagt ctttgtcctt aaaatattcc
    20161 atacagcccc agttaccaat ttgaacatta ttaattcctt ttatattatc caatatctca
    20221 aattgcttat actctttatt tctgacaatt cttggtgctg aatagattaa tttcttgtca
    20281 gtaactgctg tattagaacg cttattattc aaagtatcta tgttgtttgg atttgaaccc
    20341 attaattctt tagaaatact tgaagacgaa gaagtatcta aatcagtaga aatacttgaa
    20401 tctatagaaa tacttgagac catttgtaaa gcttttgata atgtatttat atcttcataa
    20461 tagattaagt ccacatcata atttaatact gcttctaatt gctccaaatt tttgacttta
    20521 actctaatct taggaactgt attaagtttt ttctgtttat ttactctatt tggctggtat
    20581 tttaatatat ttttcttaaa ctttctatct tttatcttta ttctttctcc atctaaaagc
    20641 tcaatacaat ctcttctcat ttgatttaac acacttatag gcatacttac accttcgtcc
    20701 aagtcaattt ctatctcttt taacttgtat ggcgtatttc caagcttttg aatttgtgtt
    20761 tctatttttt ctttacttaa agccaccttt atagcttttt caactatttt gtctccttct
    20821 actacagatt tatttccaca aaaatcactt ataaaaagtt ctggtttttt acctagttta
    20881 atactcactt ttgcacttat attagttttt atgaactctt tatcttgctg gaatgtagct
    20941 tgtactctat ccattaattc gctatcagaa gttttatata taagctgatt tcgctttgct
    21001 tctccaacaa aatctaattc tatcaaatcc cctttatggg ctattgtagt tatctctcca
    21061 tcctttatta tccttccaat agtacctccg cctaagttga ttccatcacc ttttttaagc
    21121 gtattttcta gcattactct aagtcttttg ttctttctat tataatctaa aacttttcca
    21181 atatatagac cttggttatt aggtctatta gagttcatta tatccttacc aacttcacct
    21241 aaaatatatc cttttgtaaa ttttctatta aatatagtat acaaattatt tattatttcc
    21301 tcagaaatac ttattttttt cttatctaca aattcattaa tcgcttctct atatcctgaa
    21361 accacagtag ctacatactc tggctttttc attctccctt caatttttaa agataataca
    21421 cctgcttcaa ttattttatc taaatcttct atagtactta aatctctagg actcaacaga
    21481 taatctccgt tactttttac tacttctcct gtatttatat ctatcaactc atacttttgt
    21541 ctgcatggtt gtgcacatct acctctgttt ccagacctat tgcctatcat actactcata
    21601 agacattgac cagagtaaca tacacataaa gctccatgta caaaaacttc tatatctaca
    21661 gttgtatttt cacaaatcca ttttatctca tcaacatcta attctcttgc taaaacaact
    21721 ctatcaaatc ctacactctg aaggtatttc acatcttcta aggaatgtgc taccatttga
    21781 gtgcttgcat gtagttcaaa gtcaggtaat agttttttta taagtcttgc cattcctatg
    21841 tcctgcacta ttaaagcatc aatatctata tcatataaaa attttacata ctctacaaaa
    21901 tcttctatct catactgttt tatcaatgta tttacagtaa caaagacttg cactcccctt
    21961 atgtgagagt atttaacagc ctcaatcagc tcatttctat caaagttatt tgcagatgct
    22021 ctggcactaa agtctttacc acctaaatac acagcatttg ctccgttttg aactgctgct
    22081 tttaatgaat caaaagagcc tactggtgca agtaattcta tttttttcat atttttctcc
    22141 tctatttata cttgttaggt taatataatt atatctataa ctcaaatatt tatattaaat
    22201 ttacatattt cttactatgt ttaaaatatt tgagttaatt tatatattag ttatttatac
    22261 ttggttgatt tgagttaaca tatacattat ttactatact taggatattt ctattaactt
    22321 atgcactgat taatatactt aaaatacttt aattacttta tataatattt gctatatata
    22381 aagtatctga acctcccatg actaaagtca cagggttcct gcttcacaga attttgcata
    22441 ctaaaatatg tcttactaat tctccacagg ccatcccc
//
LOCUS       DAMJVM010000054        22387 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.056, whole genome shotgun sequence.
ACCESSION   DAMJVM010000054 DAMJVM010000000
VERSION     DAMJVM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.056"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            114..230
                     /gene="rrf"
                     /locus_tag="PC523_002240"
     rRNA            114..230
                     /gene="rrf"
                     /locus_tag="PC523_002240"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            684..1754
                     /locus_tag="PC523_002241"
     CDS             684..1754
                     /locus_tag="PC523_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2475757.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGKLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGNPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVITKEVLGNETIYCLSVLNSEIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDLKICKNMYSKIKGDIYEKAI"
     gene            1738..2607
                     /locus_tag="PC523_002242"
     CDS             1738..2607
                     /locus_tag="PC523_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDF2475758.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2651..3511
                     /locus_tag="PC523_002243"
     CDS             2651..3511
                     /locus_tag="PC523_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDF2475759.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIIQFLVMVP
                     AAYAFSKIKFKGEKVLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQVSDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3542..4846
                     /locus_tag="PC523_002244"
     CDS             3542..4846
                     /locus_tag="PC523_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2475760.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNPNDSNASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQKRFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4909..6171
                     /locus_tag="PC523_002245"
     CDS             4909..6171
                     /locus_tag="PC523_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HDF2475761.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKKLPNFKSILEDCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKVKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNKHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNEEVDKALKMNDKWLG
                     DIIQATKDAGTYEDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKNFDAYV
                     DSSGISAQIIVNNLDRLTELRELLEEFKEELFIEKIFTKEEASNLGLEGDFEMVIEGI
                     EGISFGNDLEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKSGVVIEEGD
                     LRAHAATILKIFDLKLDGVEKEAFDFIV"
     gene            6357..8327
                     /gene="uvrB"
                     /locus_tag="PC523_002246"
     CDS             6357..8327
                     /gene="uvrB"
                     /locus_tag="PC523_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDF2475762.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIIEIEDELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNITPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8346..11171
                     /gene="uvrA"
                     /locus_tag="PC523_002247"
     CDS             8346..11171
                     /gene="uvrA"
                     /locus_tag="PC523_002247"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2475763.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLIATLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFAATNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPREVSKVDGSYTGQFLKKYFS"
     gene            11434..13251
                     /gene="uvrC"
                     /locus_tag="PC523_002248"
     CDS             11434..13251
                     /gene="uvrC"
                     /locus_tag="PC523_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDF2475764.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSCILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEAN"
     gene            13251..14180
                     /locus_tag="PC523_002249"
     CDS             13251..14180
                     /locus_tag="PC523_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDF2475765.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            14394..16190
                     /locus_tag="PC523_002250"
     CDS             14394..16190
                     /locus_tag="PC523_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HDF2475766.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGFAI
                     GFYNKNIVGIFVGVVSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVIIE
                     RQMAIKELLSKDDFSIHIQTLSNIIGKEQKSNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTAIILSFIFCVLGFLPVLPVFLLFIIQLGFSGFGYPKLMQTLSPLFSFSRSIQ
                     VYEKMFEVLEKETFESVYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQIIYIIA
                     CGVLMWNYHCAEALERWKGVYGNQIRDWFESIGEFEALISLTVISHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSRPWVISMVTTHDFELCDLSGNGDVEVVNYHFSEY
                     YVDDKIHFDYKIKYGRCKTTNAKQLMRMAGIL"
     gene            complement(16330..18111)
                     /locus_tag="PC523_002251"
     CDS             complement(16330..18111)
                     /locus_tag="PC523_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HDF2475767.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCIYGECPQECACEEAK"
     gene            complement(18134..20020)
                     /locus_tag="PC523_002252"
     CDS             complement(18134..20020)
                     /locus_tag="PC523_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDF2475768.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(20037..20534)
                     /locus_tag="PC523_002253"
     CDS             complement(20037..20534)
                     /locus_tag="PC523_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HDF2475769.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            complement(20852..22333)
                     /gene="cls"
                     /locus_tag="PC523_002254"
     CDS             complement(20852..22333)
                     /gene="cls"
                     /locus_tag="PC523_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDF2475770.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKIGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
BASE COUNT         8590 a         2992 c         3750 g         7055 t
ORIGIN      
        1 tcattcatta agatatatgt gtagttttta gagtgttaac tctaaaagaa ctagtcttac
       61 tagattctaa aaaattatta aaaacttaat agaaatatta agcataaaga ttatgtggtt
      121 gcaatagcag agaggataca cctgttccca ttccgaacac agaagttaag ctctctagcg
      181 ctgatggtac ttggtgggaa actgcctggg agagtaggac gtagccacgt aatttttttg
      241 ggtaaattta taaaaattaa aaaagtttta ttttttatgt ttagaccttg taaatttggg
      301 acataatata attgtaaaac ttaatatagc aaaaataaat tactcaatct cccccactta
      361 aagacctcta tcccccatag aggtcttttt tattttcaaa ccaaatcata taaaagaata
      421 aatagtttta tatgactctt cataaattta aattacattg tgatagcaaa ccatgaatat
      481 gaaaatatag aaaattatag ctaatataag ctgtatatca tcttaacttc agtatattta
      541 acaaaaaatg tttataattt aacctttatt ttaataagtt taccatatat tcacttagtc
      601 ttaatatatc ctttacaaat taagagtatt attaatctat aagtaaatag tagaattaaa
      661 ttaaatattt tgggaggata aaaatggcag atataagact agaaaatgta acaaaatctt
      721 ataataaaaa aaatacagtt atagaaaatt taaatcttac tatagaagat ggttctttta
      781 cagtattggt aggcccaagc ggatgtggca aatctactac attaagaatg attgcagggc
      841 ttgaagatat aacttcaggt aagttgaaaa ttgaagaaaa tgacgttaca aaaacggatg
      901 ctagtaaaag ggatatagct atggtctttc aaaattatgc attatatcca catatgactg
      961 ttagagaaaa tatagaattt ggtcttaaaa ataaaaaagt agaaaaagaa aaaagagaaa
     1021 aacttataac agaagttatt gatgtagtag gattaagaga atatttaaat gtgaaaccag
     1081 ggaacttatc tggaggtcaa agacaaagag tggcacttgc tagagctatg gttaaaaatc
     1141 caaaggtatt tttaatggat gaaccattgt caaatttaga tgctaaatta agaaatcaaa
     1201 tgagaacaga attaatacag ttacatcaaa aactaaaatc tacatttgtt tatgtaactc
     1261 atgaccaggt tgaggcaatg tcgatggcag ataagatagt tataatgaac aaaggaaaga
     1321 taatgcaaat aggttctcca aagaagatat atcaaaatcc taaaaattta tttgtagctc
     1381 aatttatagg gaatccttgt atgaacatac tagatataaa aaatgatatt aagtttggat
     1441 ttagaccaga aaaagcaaca ctagaaagta taaatataga tggaaaaaat gatgatgtat
     1501 ttagcttatt tggaaaagtc attactaaag aagtgttggg aaatgaaact atatattgtt
     1561 tatctgttct taatagtgag ataagagtca aaacggaaaa tgatatattt gatattggaa
     1621 gaactttaag aataagtgta ttagaaaaag atttatatta ttttgataaa aatggagaaa
     1681 gaatagaaga tttaaaaata tgtaaaaata tgtattctaa aattaaagga gatatttatg
     1741 aaaaagcaat ttaataaagc atatttatat ataacacctg ctattatagg aactttattg
     1801 tttatagtct atccaataat taagacgata tctctaagct tcaaatctgg tagtcttctt
     1861 agttcaacct ttgagaatgt gggcttgaac aattatacag aacttttaaa gaacccagag
     1921 tttatacaaa ctataacgaa tactgcaatt tatacttttt ttatggtgac tatatccata
     1981 tcattagcca tagtacttgc tgtttggcta aataaaaatt ctataataca caatttaacg
     2041 caaagtataa tctttacacc acatgtaatt tcactagtat ctgtagctgt attgtggctt
     2101 tggattatgg agccacaata tggcttttta aactggatac tatctacatt acatttacca
     2161 acatcaaagt ggctttcaag tgaaagctct gctttaatgt cactaattct aataggaata
     2221 tggaagactc ttggatacaa tattttaata gtattatcag gtcttaaatc cataccaaat
     2281 tatatatatg aagcatctaa gctagataat gcaaacaaga taactaattt ctttaaagtg
     2341 acattgccac tattatcacc aacaatattt tttcttatga taacaacaac aactgcatct
     2401 tttcaagtat ttgatgagat aagtataatg acacagggtg gtccagttgg ttcaaccaat
     2461 atgctatctt attatatata tgagtctgga tttatatatt atgacatagg gcaagcgagt
     2521 gcagcgagta tgtttttatt agctatagtt atgattgcta catatgctaa ctttagacta
     2581 ttatcaaaaa aagttcacta tcaataaaga agaaatagat attataaggc ttatcaagaa
     2641 ggaggagaaa atggatatta tacaacctat caaaaaagac aaagttgata taaaaaaaat
     2701 agcatcaaaa tcaatagaaa tattaattct attactttta tctgcaatat ttatatttcc
     2761 ttttatatgg atgttatcga catcctttaa aaatccgcaa gaaatgatgc aattaccacc
     2821 tacaattata cccaatgata tagtgttaga aaattatagt acagcatggc attcaggtcc
     2881 attttttaga tatttgctaa acagtgtatt tgtaacagga agtgttttaa ttatacagtt
     2941 tttagttatg gttccagcag cgtatgcatt ttctaagatt aaatttaaag gtgaaaaagt
     3001 gctatttgga cttttgcttg taggacttat gattccacca caagtaactt ttctaccagt
     3061 ctatatgatg atgagtaagt ttggatttat aaatacgtat gttccgctta taattccatt
     3121 tatgacatct tcatttggga tatttcttct tagacagaat tttatgcagg tttcagatga
     3181 aattattgaa gcagctaaac tagataatgc ttctgactta aaaataatgt ttagaataat
     3241 ggttcctatg gcaaagccag cagtaataac atttatatta tttaatttta tataccattg
     3301 gaataattat ttctggccct tagtaatgac aaatacagat gcaataagaa ctttaccaat
     3361 aggagttgca ctccttaaat cttcagaagg aattactgca tggaatgtaa taatggcagg
     3421 aaatataatt ctaattttac caataattct tgtttatata tttgcgaata aaaaagtaaa
     3481 agaagctttt atgtattcag ggataaaata aaaaattaaa gactaaattt ggaggatgaa
     3541 aatgaagctt aaaagaaaat tagcaacagg attagcaata acattattat taggttcttt
     3601 agttgggtgt tcaaatccta atgattcaaa tgcatctaaa ggagaaaaga ctgatagcaa
     3661 tattacaaag atagatttct gggctccttt aggtggaact aatggagaaa ctgctcaaaa
     3721 aatggttgac cagtttaata gtgaacataa ggatgtacaa gtaaatatgt taaagcagaa
     3781 agattactat gaaaatgcaa caaaactaca agcagcctta acatctaaag accaaccaga
     3841 tgtaacatta ttagaaataa ctcaaacagg tacattcgca tcagcaggtg ctttagtaga
     3901 tatgagtaaa tattttgata aaacatatca aaaaagattt ttttctggtc ttttaacaaa
     3961 ttcatattat gaggataagt ttgttggtat gccatttaat agaagcactc caatattata
     4021 cataaataaa gatatggcaa ctaaagcagg tcttgaccct tcaggtccaa aatcatggga
     4081 agagcttaag gattatgcta gcaagatgac aaataaatca gaagacactt atggttttga
     4141 aacaccaata gatatttggt tctatgaagc tatggtaatg caaagtggag gagaaataac
     4201 taatggtaaa aaagtagcat ttaataatga agcaggacaa gctccagtta agttttggca
     4261 agatatgatg aaagctggaa taatgaaaat gcctcctgga gaagattata atgcttggga
     4321 tgtagcaaaa caagattttg taaatggtaa agtaggtatg atatttacat caacagcaga
     4381 tttagcagga cttatgcaac aaacagaagg aaagtttgaa atatcaacag cattcttacc
     4441 aaaaaatcaa aaatatgcaa ctcctacagg tggtgctaat ctagttatgc ttgaaggtgg
     4501 aacagataaa gaaaaagaag caagtgcaga atttatagag tggatgacac aaacagataa
     4561 aatcgttcag tttagttcat caacaggata cttaccaaca actgaagatg caacaaactc
     4621 agaaaagtta caaacattat ataaagaaaa acctcaatat aaggttgcaa ctgaccaatt
     4681 acagtatgct gttgcacttc caatgttaaa tggatataag gaagcaactg ataagctaat
     4741 ggacgagatt aaaaagggac ttactgattt aaatgcaaat ccaaaagatg tagtaagcaa
     4801 aggaacttct gcaatgcaag cagtaataga taaaacaaat aaataattat atagacttgt
     4861 aagttgatta gaagtattca ataatatata aatgaagagg taatgtaaat gacaaaggtt
     4921 atagtattat cagtggattc tttatttgaa aaagatttgg aatttgtaaa aaagctacca
     4981 aactttaaat ctatactaga agattgttcc atagtgaaaa atataaattg tgtttatcct
     5041 actctgacat atccatgtca tactagtatg gttacaggag tatatcctaa aaaacatggt
     5101 atatatcata atgaaaaatt tgacccaaat aaagaaaata aagattggta ttggtattca
     5161 aaggacataa aagtaaaaac tattatagat gtagcaaaag aaaataatct aactacctct
     5221 agtgtattat ggcctgtaat gggagcaaat ccaaatatag attacaatat tgcagaaata
     5281 tgggctccta gtagagaaga tgacccaaga gaaacgtttg aacgaagttc cagtaaagtt
     5341 ataatggatg gtatttacaa taaacattgt cattatattg attggaaact agaacccaat
     5401 atggatttgt ttggagtaaa ctgttcagta gatataataa aggaatataa gccagattta
     5461 atgttaatac atttagctac attggaccac acacgacaca attatggctt atttaatgaa
     5521 gaagtagata aagcattaaa aatgaatgat aaatggctag gagatattat tcaagctaca
     5581 aaggatgcag gtacatacga agatacaaat tttataatac ttggcgacca tggtcatctt
     5641 agagttgata aagttataaa tccaaatgtc ttgcttaaat caaatggatt tataaaagtt
     5701 gaaaatggag aagttaagaa ttttgatgcc tatgtagatt cttctggaat atcagctcaa
     5761 ataatagtaa ataacttaga taggttaaca gaattaagag aacttcttga agaatttaaa
     5821 gaagaattat tcatagagaa aatttttaca aaagaggaag catctaacct tggtcttgaa
     5881 ggtgattttg aaatggtaat tgaaggtatt gaaggtattt catttggaaa tgatttagaa
     5941 ggaagtatta taaaagattc tgatataaaa gattataagt ttgctgttgc gacacatggt
     6001 catttaccta cgaaaggtaa tagacctcca tttatagctt ttggaccaaa tattaaaagt
     6061 ggtgtggtta tagaggaagg agacctgaga gcacatgcag caactatatt gaagatattt
     6121 gatttaaagc tagatggtgt tgaaaaagaa gcatttgatt ttattgtata agttgaataa
     6181 agtatttatt ttccatgaat tatagacttc tattgcaaag atagaagtct ttttttatgt
     6241 tttagattta ggtaaaattt aaatgggtaa aatatttaaa ataacttaaa caattttata
     6301 aaggtatgat ataataatac atgtcaggaa ctaatgttcg aaaaaggagt gatactatgg
     6361 attttaaaat aaaatcagac ttcaaaccta caggtgacca accagaagct attaaatcaa
     6421 tagtggattc tataaataga aatgagaaat tctcaacatt attaggagta acgggttcag
     6481 gaaaaacatt cacaatggca aatatcattc aacaagttaa gaaacctacc ctaataatgg
     6541 ctcataataa gactttagca gcacaacttt acagtgagtt taaagaattt ttcccagaca
     6601 atgcagttga atattttgta agttactatg attactatca accagaagca tatgtagctc
     6661 acagtgacac ttatatagag aaagatgcaa gcataaatga tgaaatagac aaacttcgac
     6721 attctgctac agcatctatt ttagagagaa gagatactat aataatatca tctgtatctt
     6781 gtatatatgg acttggtgac ccaaaagact ataaagaact tatgttatct ataagaccag
     6841 gtatgcaaag agatagagat gatgtaataa aaaggttaat agagatacaa tacgaaagaa
     6901 atgacataaa ttttacacga ggtactttta gagttagagg agatatacta gaaatattcc
     6961 cagctagtaa tgatgaaaaa gcaataagaa tagaattttt tggtgatgag gtagatagaa
     7021 taacagagat tgattatgta acagggaaga ttgttggtac tagaaatcat gttgttatat
     7081 ttccagcatc tcactatgta acaacaccag aaagaattga aaaggcaatt atagaaatag
     7141 aagacgagct tcaagaacaa attaaatttt tcaaagaaaa tgataggcta ttagaggcac
     7201 aaagaatcga acaaagaact aagtatgata tagaaatgtt aaaggaaata ggattttgtc
     7261 aggggataga gaactattct agacatatta caggaaggtc agaaggagaa agaccatata
     7321 ctttaatgga cttctttcca gatgactatt tgataattgt agatgaagca catgttacta
     7381 taccacaagt aagaggtatg tatgcaggag ataggtctag aaaaacatcc cttatagaga
     7441 atggttttag attaccttct gcacttgaca atagaccatt aaattttcaa gaatttgaag
     7501 gaaatataaa tcagatgttg tttgtaagtg ctacaccagg accttatgaa attcaacact
     7561 cagagacaat agcagaacaa ataataagac caactggact tttagaccca attgtagaag
     7621 taagacctat aaataatcaa atagatgatt tggtaggaga aataactaaa actatagaaa
     7681 aaaatgaaag agtgcttata accactttaa ctaaaaaaat gagtgaggac ttgactaatt
     7741 atcttaaaga aatagggata aaagttaaat atttacactc agatatagtt acattagaaa
     7801 gaacagaaat aataagagat ttaagattag ggaaatttga tgttcttgta ggaataaatt
     7861 tacttagaga aggattggat ataccagaag tttcacttat agcaatttta gatgcagaca
     7921 aagaaggttt cttacgttcc gaaacagcct taatacagac tattggacgt gcagctagaa
     7981 atgaaaatgg aagagtaatt atgtatgctg atagaataac tgactcaatg caaaatgcta
     8041 ttgatgaaac aaagagaaga agagatatac aaaatttata taatgaagag cacaatatca
     8101 ctcctaaaac tatacagaaa aatataagag atagtataga agctactaag gtggcagagg
     8161 aagaagttgt atatggaata tctgatacag atgataaaga tgaaataaga gctaatatag
     8221 ataaattaaa atcagagatg atggaagctg ctcaaaactt acaatttgaa agagcagcag
     8281 aacttagaga taaagtgaaa caattagaag aaaaattgga aaaatagaaa ggtaaaaaca
     8341 acattatgga agataaaatt ataataagag gagcaaaaga gcataattta aaaaatatag
     8401 acttagaatt accaagagat aaatttattg tttttacagg tttgtcaggg tctgggaagt
     8461 cttctttggc atttgataca atatatgcag aagggcaaag aaggtatgta gaaagtctat
     8521 ctgcttatgc tagacaattt ttaggtcaaa tggaaaaacc aaatgtagag tatattgagg
     8581 gtttatcgcc agctatctca attgaccaga agactacttc aaaaaaccca cgttctactg
     8641 ttggaacagt aacagaaata tatgactatt taagactatt atttgcgaga gtaggagatg
     8701 tgtattgtcc tacatgtggt gaaaaaataa gtcagatgac tattcaagaa atagttgata
     8761 aaatgttaga ttttccagat agaacgaaat tacaaatact ttcaccaatt gttagaggac
     8821 aaaaaggtac acataaaaaa gtttttgata atattaaaaa agaaggattt gtaagagtta
     8881 gagttaatgg tgaaaactat gaagttagtg atgatataat cctaaataaa aatcaaaaac
     8941 acaatataga agttgtagta gatagaatag taattaaaga tggaatagaa tcaagactag
     9001 cagattcaat tgagactgca gttaagctat ctgatggact tgtaatagcc caaataatag
     9061 atggagaaga aattcttttt tcaactaaat ttgcatgccc agaacatggg attggtatag
     9121 aagaattatc tccaagaatg ttttccttta atgctccttt tggtgcctgt gatgtttgta
     9181 atggtcttgg ggaaagtaaa gaagttgacc cagaattagt agtaccaaat aaagatttat
     9241 caataaaaca aggtgcagtg gcagcatggg gttcaacagg tgttaatgat gacacttatt
     9301 acagtaaaat gataaaaagt ctagcagagc attttaatgt atctttgaca acaccattta
     9361 aagatttacc agatgatttt gtggaagaat tactttatgg taaagataat ataattgtag
     9421 agtttactta tgagtcaaaa tttggaggaa ctagaaatta taagtcatac tttgaaggtg
     9481 taatagtaaa cttagagaga agatatagag aaactaattc agattacatg agagataaga
     9541 ttgaagaata tatggctgaa agaccatgtc caaagtgtaa aggtatgaga cttaaaaagg
     9601 aagttttatc tgttttagtt gatggaaaaa atataatgga agttacaaac ttatctgtaa
     9661 atgaattaat cgactttatg gaaaacataa atcttacaga aaaacagaga tttatagctc
     9721 atgagattat aaaagagata aaaggaagag ccatgttctt aagagatgta ggtctcgatt
     9781 atttaaattt atcaagaaaa gctggaactt tatcaggagg agaagcacaa cgtataagat
     9841 tagcgactca aataggttct gcattagttg gagtcttata tgtacttgat gagccaagta
     9901 taggacttca tcaaagagat aatgataggc ttatagccac tcttagacat ctaacagact
     9961 taggaaatac cttaatcgtt gtagagcatg atgaagacac aatgagagaa gctgattatg
    10021 ttatagacat tggtccaggt gcagggatac atggtggtga aatagttgca cagggtactt
    10081 tggatgaaat aattgaaaat gagaactcaa taacaggtca gtatttaagt ggtaaaaaac
    10141 aaatattgct tcctgagact actagagaag gaaatggaag ttttatagaa atagttaaag
    10201 catcagaaaa taatctgaaa aatattgatg taaagttccc attaggtaag tttacatgca
    10261 taacaggtgt ttcaggttct ggaaaaagta cactaataaa cgatatactt tacaaaggtg
    10321 ttgcaagtaa agtgaataga ctcaagcaaa gagctggtaa gcataaagag ataaaaggaa
    10381 ttgaaagtat agataaagtt ataaatatag accaaagtcc tattggaaga acacctcgtt
    10441 caaatcctgc tacttataca ggtgtatttg attttataag agatattttt gcagctacta
    10501 atgaagccaa agcaagaggt tataaaaaag gtagatttag ttttaatgta aaaggtggta
    10561 gatgtgaagc ttgtaagggt gatggaataa taaaaataga aatgcatttc ttaccagatg
    10621 tatatgttcc ttgtgaaatt tgtaaaggtg aaagatacaa tagagaaaca ttgcaagtaa
    10681 aatacaaaga taaaactata tctgatatat tggatatgaa tgtggaagaa gctgttgagt
    10741 ttttcgaaaa tataccaaat ataaaaagaa aattagaaac tttaatggat gtaggacttt
    10801 catatattaa gttaggacag ccttcaactc aattatcagg aggagaagca caacgtataa
    10861 aattagctgc tgaacttagt aaaagaccaa caggaaagac tttgtatata ttagatgaac
    10921 ccactactgg acttcatatg gcagatgtag acaagctaat ccaagtattg caaagactag
    10981 cagatacagg aaatacgatt atagtcatag agcataatct agatgttata aagacttgtg
    11041 actatgtaat agatttagga ccagaaggtg gagataaagg tggaactata gttgcaactg
    11101 gaacaccaag agaagtatct aaagttgatg gttcttatac aggtcaattc ttaaaaaaat
    11161 atttttctta agtcatataa attgtgaaaa attttagagc tagatatata cgctaaactg
    11221 ctcttttata agtaaattag taaaataaaa gttgtaaata gtaatacaaa gtaaagtcca
    11281 tattactctt cattgttaaa tttgtttttt aaatcaattg tagaagggaa tatggacttt
    11341 aacttgtcta aagtactgga aattgtataa ctttttaaat ttcatatttt agggtataat
    11401 tttagtataa aatttaatgt tttggagggc gagatgtttg atatacaaga gcatttaaaa
    11461 aaattacctt ctgagcccgg agtatattta atgaaagaca aatacgacca tataatatat
    11521 gtgggaaaag ccatatctct aaaaaatagg gtaaggcagt attttcaatc ttcaaaaaac
    11581 catacatcta aagtaaaatc tatggttaag aatatatata agtttgagta tattataacg
    11641 gattcagagt tagaggcatt aatacttgaa tgtaatttaa taaaaagata cagaccaaag
    11701 tataatgtag tgcttagaga tgataaaact tatccatata taaaagtaac tacaaatgaa
    11761 gactatccaa gaattttaaa agttagaaga gttcttaaag ataaggcaaa gtattttgga
    11821 ccctatacca atataactgc tgtgaatgat actttggagc ttataagtag tacttatcca
    11881 ataagaagct gtaaaattga tatagataaa gcaataaaga ataagacaag accttgttta
    11941 aatctacaca taaataaatg tttaggacct tgtacaggaa atgtttctaa agaagagtat
    12001 ggaaaaatga tagaagagat tataatgtgt ctatctggca aagaagaaaa attgatggag
    12061 cttttaaagg aaaaaatgaa tgagagttct atgaacttta gatttgaaga ggctgctgta
    12121 tatagagata agataaagag ccttgaagag atgatacaga aacaaaaaat agatgctact
    12181 gtaagtgact taaatcaaga tgtggttgca atggcaagag ctcataatga agcttgtgtc
    12241 caggtatttt tcataagaaa tggtaaaatt gtaggcaggg aacattttat attagaaggt
    12301 gtaatggata gtccaagggc ttctatatta agttcatttg taaaacaatt ttataatgaa
    12361 caagagtata tacccaaaga gttaattata gaagatgaga tagaagatag ttgtatatta
    12421 gaagaatggc tgtcatctaa aaaggggcaa aaagtaacaa taagagttcc tcaaaaggga
    12481 gagaagaaaa gcttagttga aatggttagg aaaaatgctg tagagtatct tgaaaaattt
    12541 tctgatatga ataaaagaaa atatgaaaaa agtattggag cattggaaga attaaagcaa
    12601 atattaaatt tagaaaaatt gccaataaga atagaagctt atgatatatc aaatatacaa
    12661 ggtgtagatt caattggttc tatggtagtg tacacgaatg ctaaaaaaga taagaaggaa
    12721 tataggagat ataaaataaa gacagttatt ggacctaatg attatgattc tatggctgag
    12781 attgtagata gaagattaaa acatggaaat ttacctgact tgatattatt ggatggtgga
    12841 aaaggacaag ttagtgcagt aaaaaaagta ctagaactta atgatgtaga tatacctctt
    12901 tggggaatgt acaaagatga taagcataga actaaaggat tgatatgcaa agaaaaagaa
    12961 atagagctag acaaaacaac taatttatat agatttattg ctagtataca agaagaagtt
    13021 cataattatg caataacata tcatagaagt ctaagaaata aagctttgac aaaatctatc
    13081 ttagatgata tacaaggaat aggtgaaaaa agaaagaaat cattattaaa tcactttaaa
    13141 gatgtagatg ctataaaaaa agctactatg gaagaacttt tagaagtaga tggaatgaat
    13201 aaaagcattg cagataatgt ttataatttt tttagaaagg aagcaaatta atggaaacta
    13261 gcaataaagt atcaataaga gagataataa aagagttgaa gttaaaagtg gtatacatgc
    13321 ctgatgatgt agattattat gtctactctc aagatataaa tagaccagga ttacagtttg
    13381 caggatattt tgattatttt gcatatgaaa gaatacaaat agttggaaag acagaatata
    13441 atttttttgg actaatggat agtcatattc gagaagaagt actagataga tttttctcat
    13501 atgaaatacc agcgttaata atttctaggg acttggaaat taagccagat gtaattgaaa
    13561 aggctaaaaa atataaaaga atacttttga gcacaaatag aaatactact agacttataa
    13621 atagactgtc aaattatttg gataataagt tagcaccaca cacaacaatt cacggagtgc
    13681 tagtagatat ttatggtata ggtgtactga taaaaggtga aagtagtata ggaaagtcag
    13741 aaacagcatt agaattgatt caaaggggta atagattagt tgcagatgat gctgtagaaa
    13801 taagaaaatt agatgaaagt atgcttgtgg gtcagtcacc tgaattgata aggcattttc
    13861 ttgagataag aggaataggg ataatagatg ttagaagctt atatggagta ggggcaatca
    13921 aaaattctaa aatgatagat ttagtagttc atttagaagc ttgggatgaa actaagtact
    13981 atgatagact tgggctggat aaagaatatg aagaaatact tggtgttaat gtagagaaat
    14041 tggttgtacc tgttaaacct ggaagaaata ctgctatgat attagaagta gcagcaatga
    14101 attttagaca aagaggaatg ggatacgacg cagctcaaga atttactaaa aaattatcaa
    14161 aactaattga tactaagtag tactgaataa taatgaacta aacatcattt aattaaatat
    14221 tgattaggat tatgtcctac accttataaa ttaaagtcaa gttgtatata tataatgttt
    14281 attaataaga tatattatat aaggataatt taaagtacag cgttagatta taataatact
    14341 agattaattt tatatttgaa tttcattata taacaagaaa gttggaattt tttatgggaa
    14401 tagaatattt tagtaaactt ttagaaagta gtactgaaaa agaattgctc ttaagtaaaa
    14461 agtcaaataa cataagtaca gcaagattaa tatctttttt gatagtgata gcagggtttg
    14521 ctattggatt ttacaataaa aatatagtag gtatttttgt tggagttgta tcaataataa
    14581 tatttatagc actcttagtt attcatagta aagtaaaaga agaagaaaca tattttaaaa
    14641 gtaagagcga agtattaaat aagtatatta agagatttgg agatggttgg aaggaattta
    14701 aaatagatgg taaagaatac ttaaaagatg aaaactcgca agcaaaagat ttagacttat
    14761 ttggaagagc atcattgtat caatatatct gtgtagctaa tacatcttat gggaaaaatt
    14821 ttttagccaa atatttgtgg aatgaaaatc cagatgagaa tgtaatcata gaaagacaga
    14881 tggctataaa agaactttta tcaaaagatg atttttctat tcatattcaa acactcagta
    14941 atataatcgg taaagagcaa aaaagcaatt cagatagtag tatagaatcg tttatagagt
    15001 atggagaaaa taagaatgtg tatataccaa aatggatgca tatttttact tggggtttac
    15061 caactgcgat aattttatct tttatatttt gtgtgttagg atttttacct gttttacctg
    15121 tttttttact ttttataatc cagttagggt ttagtggctt tggatatcca aaacttatgc
    15181 aaactctttc tccattattt tcatttagta gaagtataca agtatatgaa aaaatgtttg
    15241 aggttttaga aaaagagacg tttgagagtg tatatttgaa agaattacaa gacaaactct
    15301 ctaagggaag tggagtttca agaggtgtta aacaattaaa ttcaatagga aatgccgtaa
    15361 acctaaggta taatcagata atatatataa ttgcttgtgg tgttttgatg tggaactatc
    15421 attgtgcaga agctttggag cgatggaaag gtgtatatgg aaatcaaatt agagattggt
    15481 ttgaatctat tggggaattt gaagctctaa taagtttaac agttatttct catgtgaagg
    15541 aaaatacatg ctttccagta ataaaatatg aagatactcc aagattgaaa gttgaagaag
    15601 tatatcaccc attaatagca gaaaaaagtg taatagcaaa ttcaatacaa ttaaattctc
    15661 agacttgtat tatcacaggt tcaaatatgt ctggaaaaac aacattttta agaagtatag
    15721 gtgtaaactt agtgctggca tatgcaggtg ctccagtatg tgctaagagc tttgatgcaa
    15781 cctgtatggc tatatttacc tctatgagaa tacaagatga tgtaagtcaa ggaatatcaa
    15841 ctttctatgc tgagattctt agaataaaat caatgattca atatagttta aaagaactgc
    15901 caatgttggt tctagtagat gaaattttta agggaactaa ctctgctgat aggattattg
    15961 gagcaagtga ggcagtaaag aaattatcaa gaccatgggt tataagtatg gtgacaacac
    16021 atgactttga gctatgtgac ctttcaggga atggagatgt tgaagttgta aactatcatt
    16081 tctcagagta ctatgtagac gataaaattc attttgacta taagataaaa tatggaagat
    16141 gtaaaactac aaatgcaaaa caacttatga gaatggcagg gattctttaa ttaataaata
    16201 ggctaattaa taaatacatc tatttagaaa cataatttaa tttatgaaat taataaggat
    16261 tgtcttaaat tacataaaag tattttaaga caatcctttt ttaagaataa attttatact
    16321 ttgttaagac tacttagctt cttcacaagc acattcttgt ggacactctc cataaataca
    16381 actcttatca aagtaatgag tatgaaggta tttatgagct acatcactat ttggttctcc
    16441 aaagaagtct ttataaatag cttgtaaatc aggattttca tgagatttac gaatagcctt
    16501 atttctatct atttcatata acgcatttgc acgtctatca actatttcag tattaccatg
    16561 gtgataaggt tgaccagcac ctccaacaca tccaccagga cacgccatga cttctatgat
    16621 atggtattta cattctccac ttctaacctt atccataatc tttctagcat ttttaagtgt
    16681 atttacaata catacattaa ctgtagttcc atcaacatct atacttgctt ctctaattcc
    16741 tttaagacct ctaacattag tgaagtttac attatctaaa gtcttattag ttatcttctc
    16801 atatgaagta cgtagggcag cttctaaaac tccaccactt gctccaaata tagatgcagc
    16861 acctgtagat tttcctagag gattatcaaa atcttggtct tgaagattag gtaaatctat
    16921 agcagcttct tttatcatct tagctaactc tctagtagtt atagaaaggt caacatctag
    16981 tattccagat tcacttaatt cttctcttga agcttcatat tttttagcta cacaaggcat
    17041 tacagataca acataaagtt cctcaggatt gattcctaat attttaggtg catagtagtt
    17101 tttagctata gaaccaaaca taccttgagg agatttacag ctagatgcta agtttaactt
    17161 gtctggataa ttgtgttcta agaagtttac ccatgcagga caacagcttg ttaatatagg
    17221 aagattttca cctttttgga ttctttctat aaactcagta gcttcttcca ttatagtaaa
    17281 gtctgcacca aagtttgtat caaatacatg ttcaaatcca agtgctttta aagcagctac
    17341 catttttcct gtagagattg accctggttc taatccaaac tcttcaccaa tagcaaccct
    17401 tacagcagga gcaacttgca ctactatagt cttttctttt ttatttaaaa catcccaaag
    17461 tttaggtaca ttatccactt ctgttaatgc tccagttgga cagacagata tacattgacc
    17521 acagaatgta cactctgttt caaccatatc agagttaaag aatgtactaa ctagagtatt
    17581 aaatccacga ttaactccag ataatgctcc tactgtttgt atgtcattac atacagtctc
    17641 acatcttcta cataatatac acttagaatg gtctttaact aaagatttag tagaagtatc
    17701 tttacctgca aaagatttag aaccttgata tcttattttt cttactccta aatcagcagc
    17761 tatagtctgt aattcacaat ctccattttt ttcacataca aagcaatctt gtggatggtc
    17821 tgatagtaat aattcaacaa tagttctacg agcatttaat gcttttgcac tattagtctg
    17881 aactttcata ccttccttta taactgttcc acaagctgga actaatcctc tctctgtctc
    17941 aaccatacaa acacgacaag aagcacaagt atcaagctta tctatttcat tcatatgcaa
    18001 atgacaaagg ttgtgtattt ttatatttat taattttgca gcatctaaga tagaagttcc
    18061 actaggtgct gaaacgtgtt tgccattaat agttaaatta actaaactca ttgttattcc
    18121 tcctccaaat aagttatttc ttgattatag cgttaaatgt acaattatct atacaagcac
    18181 cacattttag acacttagaa gtatctattg tatgttgttc tttaacagaa cctgttatac
    18241 aaccagcagg acacactcta gaacattttg tacaaccctt acagtcatct gttataaagt
    18301 aagaaagtaa gttctgacat ttaccagcag gacatttctt atctacaaca tgagctatat
    18361 actcatcttt gaaatattga agagtactta aaacagggtt tggagcagct ttaccaagac
    18421 cacataatga agcagtttgt atagtttcag caaggttttc taggtcatct aaatcttgta
    18481 gagtaccatt accttctgta attttagtta atatttctaa caatcttgta gtacctatac
    18541 gacaaggtgt acatttacca caagattcct caacagtaaa ctcaaggaag aatttggcta
    18601 tatctaccat acagtcagat tcatcaagaa caagcatacc accagagccc atcattgaac
    18661 cgatagaact taatgaacca aagtctatag gagtatctaa atgttcagtt ggtatacatc
    18721 caccagatgg gccacctgtt tgaacagcct taaatttctt accttcaggg attccaccac
    18781 caatttcaaa tacaatatct cttaaagtag tacccattgg aacttcaaca agccctacat
    18841 tttctacttt tccaccaaga gcaaaaactt tagtaccact agaatcttca gtacctaagt
    18901 tggcaaacca atctccacct ttaagtatta ttgcaggtat attagcaaaa gtctcaacgt
    18961 tatttaagca agtaggagat ttccatagac cactcttaga agaactataa gtcttcattc
    19021 taggttcacc acgtctacct tcaattgaat gcattagagc tgtaccttca ccacaaacga
    19081 aagcacctgc accatatttt aattctaatt taaagttaaa tccagtacct aagatatttt
    19141 caccaagtaa accgtatttt tcagcttgag ctatagctac ttttaatctt tctatagatt
    19201 ttggatattc tgctcttata tatatgtatc ctgtatcaga acctatagca tatccacata
    19261 ttgccatagc ttctagtaca ctatgagggt caccttcaag tacagatcta tccataaatg
    19321 cacctgggtc accttcatca gcattacata caacaaactt tttatctgtt ggaccagctg
    19381 gattttttga agcagcttcc catttagagc ctgtagggaa accagcacca cctctaccac
    19441 gaaggccaga agtttttacc tctgcaataa cttcctgagg agtaagagaa gttaaacatt
    19501 tgcctagagc taaataacca tcattggcaa tatattcttc tatactatct gggtctataa
    19561 gaccacaatt ttttaaagct atacgtaact gttttttgta gaaagacatt tcttcttgtt
    19621 cttctacttt tttacttaaa gattcttcct catataataa ttcctcgaca actgtatttc
    19681 taattaagtg gctttgaact atcttttcag catcagatgg ctcaactttt acgtagaaaa
    19741 cgttatcagg ataaactttt acgataggac cttgagcaca gaaaccaaaa caacctgtca
    19801 aacgaacact aactttatct tggattcctg atttttttat ttcattttct aattcactaa
    19861 ctatttctag actgtttgaa gatgtacatc cagtatcaca acaaacaagt aattctcttc
    19921 ttagtacatt ttccttagaa taattttttc ttaagctaag attaggtttc agctcatcag
    19981 ctaaaacttt taattcatca aatgaattta ctttacgcat tatttattca cccctcttaa
    20041 caattcaatt ctaattctct ataagtatct aatatttcgc ttacttggtc aggtttcact
    20101 ttaccataaa ctttgccatt tacagtaaca actggagcta aaccacatgc ccccaaacat
    20161 cttaaacctt ctaaagaaaa tttaaaatca ctagtagttt ctccaacttt tatgccaagc
    20221 tgtttttcaa aagcacttaa gattttgtca gcacctttta caaaacatac agtccctaga
    20281 cagacactga ttttatactt acctacaggt tctgtagtaa aataagaata gaatgtaaca
    20341 accccatata cctttgcagg agctactcca agttttctag caacatgaag ttgaacttct
    20401 tttggaagat acccaaatat accttgggct tcatgtagaa cttgtattaa cgcaccttcc
    20461 tttgttgcaa gagagtcaat aaatatatct agttcgtcaa ataattgctt gttgtgtgaa
    20521 ataaaatcgc acataacaaa tccccctttt ctttttaaag ttattatttt tcaaaaaata
    20581 attaaccatg agttgacccc aacattatgt gggtgaacaa aaccaaaaca ataaagaaag
    20641 aataatatac aacaatttct ttatttataa aaacaatgca aaaaagttac ctattaaaaa
    20701 taaaaactat actagtctat tataatataa aataattcag aatagttaaa aaaaataaaa
    20761 aaaatgagct aaattcaata aaaaaaaaat aatatatata aaaaacttta aaaaataaac
    20821 caaaatgtaa aatttttata aaataaaaga tttataaaac tgctgataat agtcttgaaa
    20881 ttgattcctt gatttttata taagtagacc tagaattata tatatctaat gttatttcct
    20941 ttgaatttaa aatatcattt tcaaatataa ttctttgttc caatgctttt tttgaagagt
    21001 acataaatgc attcacttcg aaattaagct caaaacttct gatatccata ttagctgttc
    21061 ctatagagca tatagagtca tctataacta ttgtcttagc atgtaaaaat gcgttttgac
    21121 cataagtgta gattttagct ccaaacttta aaagctcccc agcatatgaa tatgatgccc
    21181 agtatacaaa tggatggtca ggtttagaag gaatcataac tctcacatca acaccagata
    21241 aacaagctat ttttaaagta tctataaaag ttgagtcaag tattaaataa ggactctgta
    21301 tgtatatata ctttctagct ttctgaatca ttttaaggta accatattta atctcatcaa
    21361 gttctgatat atctggacca cttgatacta tctgaatccc aatattttca gaaggatgat
    21421 tagaatttgt atcttcttct ataaagtact tttctaaatc taaatcttct ttagttgtgt
    21481 atctccaatc gaggatgaat ctcatattta ggtcaattac acaatcacca gtaagcttag
    21541 tatgtgtatc tctccataga ccaaattttg gatttctacc taaatattca tcaccaacat
    21601 tatatccacc tacaaatccc accttaccat caatgacaac aatttttcgg tgatttctat
    21661 agtttaagtt aaaatttaca attttcaaga atgatgggaa gaagcttcca attttaacgc
    21721 cactttcttt aagtctggaa agtgtactat tttttagtgt tctaccgcca actgcgtcaa
    21781 ataaaagtct tacttcaaca ccttctttag ctttgtctat aagaatgtct attatttcag
    21841 ttcctatttt gtcatctttg aaaatgtaaa attgtatgtt tatatatttt ttagcttttt
    21901 taagttcttc aagaagactg ttaaaaaatt gactagattc agcataaatc cacacatcgt
    21961 tattattggt ataatgagca ttatttgaat tagccaatgc ataaatcata tccttatgtt
    22021 gatgtatatc tgaatcaatt tcagatgtag attggagttt aacctgagta tctagtatat
    22081 tgcttttgat tatcttatca tccttttctt tcaatctaaa catgttgttt tttgaaatat
    22141 ttctaccaaa agctatataa agtatgaatc caagagcagg aatcaatact aatataagaa
    22201 gccatgttaa agttgtattt atgtttcttt tttctctaaa gataaggttt agtataacca
    22261 tgaaattaat tatatatatt gtagtaactg ctatttcata tattgacatt tcgaaaaatg
    22321 atatatcaaa cataaaattt cctcctttaa atatttatta aaattaagta attatatata
    22381 acaatac
//
LOCUS       DAMJVM010000055        21942 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.057, whole genome shotgun sequence.
ACCESSION   DAMJVM010000055 DAMJVM010000000
VERSION     DAMJVM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.057"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            366..677
                     /gene="rpsJ"
                     /locus_tag="PC523_002255"
     CDS             366..677
                     /gene="rpsJ"
                     /locus_tag="PC523_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDF2475771.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            769..1398
                     /gene="rplC"
                     /locus_tag="PC523_002256"
     CDS             769..1398
                     /gene="rplC"
                     /locus_tag="PC523_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDF2475772.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1428..2051
                     /gene="rplD"
                     /locus_tag="PC523_002257"
     CDS             1428..2051
                     /gene="rplD"
                     /locus_tag="PC523_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDF2475773.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNIIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2051..2341
                     /gene="rplW"
                     /locus_tag="PC523_002258"
     CDS             2051..2341
                     /gene="rplW"
                     /locus_tag="PC523_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDF2475774.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTASFKKAVVKLTADSKEIEFFQGM"
     gene            2371..3201
                     /gene="rplB"
                     /locus_tag="PC523_002259"
     CDS             2371..3201
                     /gene="rplB"
                     /locus_tag="PC523_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDF2475775.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3236..3517
                     /gene="rpsS"
                     /locus_tag="PC523_002260"
     CDS             3236..3517
                     /gene="rpsS"
                     /locus_tag="PC523_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDF2475776.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3549..3884
                     /gene="rplV"
                     /locus_tag="PC523_002261"
     CDS             3549..3884
                     /gene="rplV"
                     /locus_tag="PC523_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDF2475777.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3908..4723
                     /gene="rpsC"
                     /locus_tag="PC523_002262"
     CDS             3908..4723
                     /gene="rpsC"
                     /locus_tag="PC523_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDF2475778.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4759..5190
                     /gene="rplP"
                     /locus_tag="PC523_002263"
     CDS             4759..5190
                     /gene="rplP"
                     /locus_tag="PC523_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDF2475779.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5192..5395
                     /gene="rpmC"
                     /locus_tag="PC523_002264"
     CDS             5192..5395
                     /gene="rpmC"
                     /locus_tag="PC523_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDF2475780.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5419..5673
                     /gene="rpsQ"
                     /locus_tag="PC523_002265"
     CDS             5419..5673
                     /gene="rpsQ"
                     /locus_tag="PC523_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDF2475781.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5699..6067
                     /gene="rplN"
                     /locus_tag="PC523_002266"
     CDS             5699..6067
                     /gene="rplN"
                     /locus_tag="PC523_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDF2475782.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6091..6396
                     /gene="rplX"
                     /locus_tag="PC523_002267"
     CDS             6091..6396
                     /gene="rplX"
                     /locus_tag="PC523_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDF2475783.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6426..6968
                     /gene="rplE"
                     /locus_tag="PC523_002268"
     CDS             6426..6968
                     /gene="rplE"
                     /locus_tag="PC523_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDF2475784.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEIISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6986..7171
                     /locus_tag="PC523_002269"
     CDS             6986..7171
                     /locus_tag="PC523_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDF2475785.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7204..7602
                     /gene="rpsH"
                     /locus_tag="PC523_002270"
     CDS             7204..7602
                     /gene="rpsH"
                     /locus_tag="PC523_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDF2475786.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7633..8175
                     /gene="rplF"
                     /locus_tag="PC523_002271"
     CDS             7633..8175
                     /gene="rplF"
                     /locus_tag="PC523_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDF2475787.1"
                     /translation="MSRIGVKPIIVPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8211..8579
                     /gene="rplR"
                     /locus_tag="PC523_002272"
     CDS             8211..8579
                     /gene="rplR"
                     /locus_tag="PC523_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDF2475788.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8600..9109
                     /gene="rpsE"
                     /locus_tag="PC523_002273"
     CDS             8600..9109
                     /gene="rpsE"
                     /locus_tag="PC523_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDF2475789.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9124..9309
                     /gene="rpmD"
                     /locus_tag="PC523_002274"
     CDS             9124..9309
                     /gene="rpmD"
                     /locus_tag="PC523_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDF2475790.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9342..9785
                     /gene="rplO"
                     /locus_tag="PC523_002275"
     CDS             9342..9785
                     /gene="rplO"
                     /locus_tag="PC523_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDF2475791.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9829..11097
                     /gene="secY"
                     /locus_tag="PC523_002276"
     CDS             9829..11097
                     /gene="secY"
                     /locus_tag="PC523_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDF2475792.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGQVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11114..11764
                     /locus_tag="PC523_002277"
     CDS             11114..11764
                     /locus_tag="PC523_002277"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDF2475793.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVNYYSKQGIIADIKGDQAIDKVFEDIVTALGSGK"
     gene            11765..12511
                     /gene="map"
                     /locus_tag="PC523_002278"
     CDS             11765..12511
                     /gene="map"
                     /locus_tag="PC523_002278"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDF2475794.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12533..12802
                     /locus_tag="PC523_002279"
     CDS             12533..12802
                     /locus_tag="PC523_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HDF2475795.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12840..13058
                     /gene="infA"
                     /locus_tag="PC523_002280"
     CDS             12840..13058
                     /gene="infA"
                     /locus_tag="PC523_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDF2475796.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13094..13207
                     /gene="rpmJ"
                     /locus_tag="PC523_002281"
     CDS             13094..13207
                     /gene="rpmJ"
                     /locus_tag="PC523_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDF2475797.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13360..13731
                     /gene="rpsM"
                     /locus_tag="PC523_002282"
     CDS             13360..13731
                     /gene="rpsM"
                     /locus_tag="PC523_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDF2475798.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13767..14165
                     /gene="rpsK"
                     /locus_tag="PC523_002283"
     CDS             13767..14165
                     /gene="rpsK"
                     /locus_tag="PC523_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDF2475799.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14197..14820
                     /gene="rpsD"
                     /locus_tag="PC523_002284"
     CDS             14197..14820
                     /gene="rpsD"
                     /locus_tag="PC523_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDF2475800.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14898..15845
                     /locus_tag="PC523_002285"
     CDS             14898..15845
                     /locus_tag="PC523_002285"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDF2475801.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICSPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15866..16207
                     /gene="rplQ"
                     /locus_tag="PC523_002286"
     CDS             15866..16207
                     /gene="rplQ"
                     /locus_tag="PC523_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDF2475802.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16327..17160
                     /locus_tag="PC523_002287"
     CDS             16327..17160
                     /locus_tag="PC523_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDF2475803.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDGMNTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISGDILTVDEMVMELCR
                     L"
     gene            17148..18014
                     /locus_tag="PC523_002288"
     CDS             17148..18014
                     /locus_tag="PC523_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDF2475804.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDISFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEEFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKSLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFIGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18008..18811
                     /locus_tag="PC523_002289"
     CDS             18008..18811
                     /locus_tag="PC523_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDF2475805.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMISIFVVNKFWP
                     YIVVLLCLLAMIKLANIPIKYIVKGVKPLKWIILFTFFINIFFLPGDEIWSFGFLTIT
                     SQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18844..19575
                     /gene="truA"
                     /locus_tag="PC523_002290"
     CDS             18844..19575
                     /gene="truA"
                     /locus_tag="PC523_002290"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDF2475806.1"
                     /translation="MRNIKITIQYNGKNYCGWQKQLDSLGIQGTIERAIYDITKEKVS
                     LIGSGRTDSGVHAIGQIANFKTNSSISIESIPMALNAKLPKDISVIDACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKLLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDIIRKDDLITFEISGNGFLYNMVRIIVGTLVDIGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19695..20126
                     /gene="rplM"
                     /locus_tag="PC523_002291"
     CDS             19695..20126
                     /gene="rplM"
                     /locus_tag="PC523_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDF2475807.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20155..20547
                     /gene="rpsI"
                     /locus_tag="PC523_002292"
     CDS             20155..20547
                     /gene="rpsI"
                     /locus_tag="PC523_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDF2475808.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
     gene            21056..21760
                     /gene="cwlD"
                     /locus_tag="PC523_002293"
     CDS             21056..21760
                     /gene="cwlD"
                     /locus_tag="PC523_002293"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HDF2475809.1"
                     /translation="MRKYIKHIIFSFAMICLVVVSIFEIKNISEDVIKYMPVTNKTII
                     LDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGLVILTREDDSSLYKEEN
                     NKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYGAQTFYPKDKQDSKELS
                     KCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGFLSNEKESKLLTDETYQ
                     EKIAWAIYIGIQKYLS"
BASE COUNT         8799 a         2565 c         4504 g         6074 t
ORIGIN      
        1 ctttactagc taatggattc aatataaaaa agagggggta acacctctct tttttgtttt
       61 ttattgacaa gtagctctaa agcatatata ctaaactagt gccttgagat agattaacaa
      121 tcaatttaag gttatgatgt tgtaatagaa aataaaattg aaaaaaagat aaaaaaaact
      181 tgaaaaaatg taacagatga tataaaatat aatagtgtgc ttgagagatt aagaaaataa
      241 gaattaaatc ctagtgaaat acgaactgat atcgatttga aagttacaac acacccggag
      301 cagtgtatcg gtattggttg tacgcagctt gaaaattaaa cacgtgataa aggagggaaa
      361 acaaaatggc taagaatgaa aaaataagaa taagattaaa atcatatgat cacaaattat
      421 tagatttttc agcaggtaaa atagttgaaa ctgctaaaaa ggctggatca caagtttcag
      481 gacctgtgcc tctaccaaca gaaaaacaag ttgtaactat attaagagct gtacataagt
      541 acaaagactc tagagaacaa tttgaaataa gaactcataa aagactaatc gacatagcta
      601 atccaacacc taagacagtt gactcattaa tgagattaga cttaccagca ggtgtagata
      661 tagaaataaa attataagaa cccctaagat attatcctat agatgattat cttagaatga
      721 ttactctaaa agatgtaatc cgctgtaaga attataggag gtgctaatat gaaaggaata
      781 ttaggcaaaa aagttggtat gactcaaata ttcactgata aaggtgtagt tatacctgta
      841 acagctgttg aagcaggacc tatggtcgtt actcaaataa aaacagttga taaagatgga
      901 tacaatgcta tacaaatagg ttttgaagat gctaaagaaa aagctttaaa caaacctaaa
      961 aaaggacatt tagctgctgc taatgtttta aagaaacatt taaaagaatt tagagtagat
     1021 tctgtagaag gatacacagt aggacaagaa ataaaagctg atgtatttga agcaggtgca
     1081 aaaatagatg ttactggaat aagtaaaggt aagggattcc aaggtccaat aaaaagacat
     1141 ggacaaagta gaggtcctga aactcacggt tctagatacc acagaagacc aggttcaatg
     1201 ggagcttgtt cttacccagg tagagtattt aaaaacaaaa aattagctgg acacatggga
     1261 agcgtaaagg taacagttca aaacttagag gttgttaagg tagatgctga taagaacctt
     1321 atattagtta aaggagctat accaggagct aaaggttcag tagtaactat aaaagaagct
     1381 ataaaggttt ctaaataatg actaacctag agaaaggagg aataacaatg ccaaaattaa
     1441 atgtactaaa tgttagtgga caaaacgttg gagaaataga attatcagat tctatatttg
     1501 gcgtagaagt taatggacat gttttatatg aagttgtaaa aaatcaatta gcaaataaga
     1561 gacaaggaac tcaatctgct aaaactagag cagaagttag aggtggcgga agaaaacctt
     1621 ggaaacaaaa aggaacaggt agagcaagac aaggttctac aagatctgta caatgggtag
     1681 gtggaggagt tgcttttgca cctaaaccaa gaagttacaa atatacatta cctaagaaag
     1741 taagaagatt agctatgaag agtgctttat cttcaaaagt tcaaaatagt gaagttatag
     1801 tattagatgc attaaacatg gatgctccta agactaaaga atttgctcaa atattaaaca
     1861 atataaatgc tgctaaaaaa gctttagtag ttatagctga caaaaacgat aacataataa
     1921 aatcagctag aaatatagaa ggtgttcaaa ctgcattagt taatactatg aatgtatatg
     1981 atatattaaa atatgattca tttataataa ctacagatgc tgttaaaaaa gtggaggagg
     2041 tgtacgcata atgactaatc cacatgatgt aataataaga ccagttgtaa ctgagcacag
     2101 tatggctgaa atgggtgaaa aaaaatacac ttttgttgtg gctaaagatg cgaacaaaac
     2161 tgaaataaaa aaagcagtag aaaaagtatt cggagttagt gttgataaag taaatacttt
     2221 aaactacgat ggaaaagtta aaagaatggg tagaactcaa ggaagaacag caagctttaa
     2281 gaaagcagta gttaaattaa ctgctgatag taaagaaata gaattcttcc aaggaatgta
     2341 gtattataac aaacgaagga gggaaaacag atggctataa aaaagtttag accaacttct
     2401 cctgccttaa gacaaatgac agttttagtt tctgatgaaa taacatgcaa tcaaccagag
     2461 aaatctcttt tagttaactt aaagaaaaat gctggtagaa acgtacatgg tagaataact
     2521 gttcgtcata gaggtggagg acaaaaaaga aaatatagaa taatagattt taaaagagat
     2581 aaagatggta tacctgctaa ggttgctact atagaatatg atccaaacag aactgcaaat
     2641 atcgcattat taaactatgc tgatggagaa aaaagatata tattagctcc agttggaatt
     2701 aacgttggag atactatatt atcaggatta ggtgctgata taaaaccagg aaactgttta
     2761 gcattaaaag atatgccagt tggtactatc attcataata tagaattaaa gccaggaaaa
     2821 ggagctcaat tagttagatc tgctggagtt tctgcacaat taatggctaa agaaggaaaa
     2881 aatgctttat taagattacc atcaggtgag atgagattag taagcataaa ctgtaaagct
     2941 actataggtc aagttggaaa tatagagcat ggtaacgtag ttatcggtaa agcaggtaga
     3001 aaaagacata tgggaataag acctactgta agaggttctg taatgaaccc taatgaccat
     3061 ccacacggtg gaggggaagg tagatctcca ataggtagac catctcctgt tactccatgg
     3121 ggtaaaccag ctcttggata caaaactaga aagaaaaata aagcttcaaa taaactaata
     3181 gtatcaagaa gaactaagta gtaaatttat ttttgctcgg aaaggaggaa tataaatgtc
     3241 aagatcaact aaaaaagggc cttttgtcca tgcaagactt ttaaagaaga tagaagctat
     3301 gaatgctagt ggaaataaag aagttataaa aacttggtca agatcttcaa ctgtatttcc
     3361 acaaatggta gaaaatacta tagctgttca tgatggaaga aaacatgttc cagtatatat
     3421 aacagaagat atggttggac ataaattagg tgagttcgtt cctactagaa ctttcaaagg
     3481 acataaggat gacgaaaaat ctaataagag aaaataatta aattatgact aagaaaggag
     3541 gaataacaat ggaagcaaaa gctactgcta aatacgtacg tgtatcacca agaaaagcag
     3601 gccaaatatg tgaccttgtt agaggaaaga atgttgatga agcattagca atattaaagt
     3661 ttactccaag aggagcagct tcaataatag ctaaagttgt aaaatcagct aaagctaacg
     3721 cagaaaacaa tcacgaaatg gatactgaaa aattatatat agcatcaata gttgctaatc
     3781 aaggaccaac aatgaagaga tttatgccta gagctatggg tcgtgcaact acaataagaa
     3841 aaagaacttc tcatatagag gttgttgtta aagagaaaaa ataattaata gataggaggg
     3901 aaagttaatg ggtcaaaagg ttaatccaca cggcttaagg gtcggtgtta taaaagattg
     3961 ggactcaaga tggttcgcta ctgataaaaa agagttcgga aacttattat tagaagacca
     4021 taatatacgt aaattcttaa agaaaagatt atactcagct ggagttgcta agatagaaat
     4081 agaaagatca gctaacaaaa taaaaatgga cttacatgtt gctaagcctg gtgtagttat
     4141 aggaagagct ggtgcaggaa tcgaagcatt aaaagctgaa ttagaaaaaa tgacaaagaa
     4201 aactataata gttaatatag tagaagtaag aagtacagat aaaaatgctc aattagtagc
     4261 tgaaaatata gctttagcta tagagagaag ggttgctttc agaagagcaa tgaaacaagc
     4321 tatacaaaga gcaatgaaat ctggtgctaa agggataaaa gtttctgctt caggaagatt
     4381 aggtggagct gaaatggcta gaactgaagg ttatagtgaa ggaaatgtgc cactacaaac
     4441 tttaagagca gatatagatt atggtttcgc tgaagctgat acaacttatg gaaaaatcgg
     4501 tataaaagtt tggatatgta atggtgaagt tttaccaact agagacggtg taaatccaag
     4561 agaagaaagc agaaagagcg atagaagaga caacaagaga gataatagaa gaaacgatag
     4621 aagaggaaat gacagaagag gaaatgataa cagaggaaac tacagaggac aaagacctca
     4681 aggtggatca agacctcaaa gaactgaaaa caaaggaaat taataatagt taggttcaag
     4741 gaaggaggaa aaatactcat gttaatgcca aaaagagtaa agcgtcgtag agttcataga
     4801 ggaagcatgg ctgggcaagc tcataagggt aataaagtta cttatggtga gtttggatta
     4861 gtagcattag aagcttcttg gataacttct aatcaaatag aagctgccag aatcgcgatg
     4921 actagatata taaaaagagg gggaaaggtt tggataaaaa tattccctca taaaccagta
     4981 acaagaaaac cagcagagac tcgtatgggt gcagggaaag gttctccaga atattgggta
     5041 gctgtagtta aaccaggaag agttatgttt gaattagcag gtgtttctga agataaagca
     5101 agagaagcta tgagacttgc tgcacataaa ttaccaatca aatgtaaatt tgttaaaaaa
     5161 gaagatttag aagtaaaggg tggtgaatag gatgaaagct aaagaattaa gagatttaac
     5221 aagcgaagag ctaatgaaca aattaaacga cttcaaaagt gaattattta gcttaagatt
     5281 ccaattagct actggtcaat tagaaaatac agctagaata aagtttgtta agaaggatat
     5341 agcaaaagtt aaaactgttc ttgctgaaag aaagttatac gaaactagag cttaatttgg
     5401 aaaggaggct ttttaaacat ggaaagagga agaagaaaag ttagaatagg ccgtgttgtt
     5461 agtgataaga tggataaaac aatagtagta gctgttgaag aattcgtacg tcatccacta
     5521 tataataaac gtgttaagag aactaagaaa ttcaaggctc atgatgaaaa gaatatatgt
     5581 aacatcggag atagagttaa aataatggaa actagaccat tatctaaaga taagagattc
     5641 agactagttg aagttgttga gaaagttaag tagtttttga agaaggaggg attacgatat
     5701 gatacaacaa gaatcacgtc taagagttgc tgataactca ggagctaaag aacttttatg
     5761 tatacgtgtt ctaggcggaa gtaaaagaag atatggtaac ataggcgacg ttatagttgc
     5821 tactgttaaa agtgcaacac ctggtggagt tgtaaaaaaa ggtaaggttg ttaaagctgt
     5881 tatagtaaga agcaaacaag gcgtaagacg taatgacgga agctatatat cttttgatga
     5941 aaatgctgct gttataataa aggacgataa aactccagta ggaactcgta tattcgggcc
     6001 tgttgctaga gagttaagag acaatgaatt catgaaaata gtttctcttg ctccagaagt
     6061 actataatta aggaggtgca ataggacatg atgcgtgtta aaaaaggtga cactgttgta
     6121 gttatagcag gtaaagataa aggtaaaaaa ggttcagttt taaaggtata tcctaaaact
     6181 agcaaagtgt tagttgaagg tgtaaatgta ataactaaac atcaaaaacc aagtgctatg
     6241 aaccaacaag gtggaataat aaataaagaa gccccaatac acatatctaa tgtaatgcca
     6301 tttgatcctg aaacaggaaa aggcgttaga gtaagatatg aagtaaaaga tgggaacaaa
     6361 gtaagagtat cagcaaagag cggaaaagaa ttataataaa tattgcttga aaggagggac
     6421 cttaaatggc ttctagatta caagaaaaat acatgaaaga agttgctcct gctttaatgg
     6481 agaaatttgg atacaaaaac gtaatggaga tacctaagtt aaataaaata gttattaaca
     6541 tgggtatagg tgacgctaga gaaaatccaa aaggattaga aaaaggcgtt gaagaattag
     6601 aaataatatc aggacaaaag cctgttataa ctaaagctag aaaatctgtt gctaacttca
     6661 aattaagaga aggaatgcca ataggaacaa aagttacatt aagagctgat aaaatgttct
     6721 actttatgga caaattagtt tcagtttctt taccaagagt tagagacttt agaggagtta
     6781 accctaatgc ttttgatggt agaggaaatt atgctttagg agttaaagaa caattaatat
     6841 tccctgaaat agaatatgat aaaatagata aagtaagagg aatggatata atatttgtta
     6901 ctacagcaaa gactgacgaa gaagctcgtg aattattaaa attattagga atgccatttt
     6961 ctaagtaagc aaaggaggga ttcccgtggc tagaaaagcg atggttgtta aacaacaaag
     7021 aaagcaaaag tacgctacta gagaatacac tagatgtact atatgcggaa gaccgcattc
     7081 agttttgaaa aaatttggta tatgccgtat atgctttaga gaattagctt ataaaggtca
     7141 aatacctggt gttagaaaag caagctggta gaagcttgat tagaatggaa ggagggcaac
     7201 aaaatgacaa tgacagatcc aattgcagat atgcttactc gtataagaaa tgctaatgtt
     7261 gttaagcatg aaactgtaga tgttccagct tctaatatga agaaagaatt agctagaatc
     7321 ttattagaag aaggtttcat aagaggttat gatgttatag aagatggaaa acaaggtatc
     7381 ataagaatac aattaaagta cggacaagaa ggcgaaagag ttataacagg tcttaagaaa
     7441 atatctaagc ctggaatgag agtttacgct gctaaccatg agataccaaa agtattaaac
     7501 ggattaggaa tatcagttat atctacttca aaaggaatat taactgacaa gcaagctaga
     7561 aaagaaaatg ttggtggaga agtaatctgc tacgtttggt aatcaatatg aacaatgtaa
     7621 ggaggtgcga ccatgtcaag aataggtgtt aagccaataa tagtaccagc aggtgttgaa
     7681 gttactatag ctgaaggaaa tttagttaca gtaaaaggtc caaaaggaac tttaactaaa
     7741 caattaagtg ctgagttaaa cataaaaaaa gaagaaaata caataatggt tgaaagacca
     7801 actgataata aaaaacatag atctttacac ggactaacta gaactttatt agataatatg
     7861 gttgtaggtg taaatactgg tttcgaaaag aaattagaat taaaaggtgt tggatataga
     7921 gctcaaaaac aaggaaagaa attagttatg aacttaggtt tctctcatcc agttgagatg
     7981 gaagatccag aaggaataac tgttgaagct ccaaaccaaa cagagttaat agtaaaaggt
     8041 atagacaagc aattagtagg gaactatgct gctaagataa gagcttggag aaagccagag
     8101 ccatacaaag gtaaaggtat aaaatacgtt gatgaagtaa tcagacgtaa agaaggaaaa
     8161 actggtaaaa aataatacca aaacttaaac gctagaaagg agtgagctag gtgttaaaaa
     8221 aggctgataa aaatgctaat agacttcaaa gacataagag agttcgtaga aaaataagcg
     8281 gtacttctca aagaccaaga ttatgtgtat ttagaagtgc taacaatata tatgctcaaa
     8341 taatagatga cacaaaaaga gtaactctag ttgctgcatc ttctttagaa gcagaagtta
     8401 agagtgctgt caatcataca ggaaacaagg aagcagctaa aaaagttgga gaattagttg
     8461 ctaaaaaagc tgttgaaaaa ggaatcactg aagttgtatt tgacagaggt ggatacttat
     8521 atcatggaag aattcaagaa ttagctgaag gtgctagaga agctggtctt aagttctaat
     8581 acgaaggagg gaaaaataca tgctacgtcg taagccgata gatgcaggac aacttgactt
     8641 acaagaaaaa gttgttgaag taagacgtgt tactaaggtt gtaaaaggtg gtagaaactt
     8701 cagatttgca gcattagtag ttgttggaga tgaaaacgga cacgttggta taggtgctgg
     8761 gaaagcaatg gaagtaccag acgctataaa aaaagcagtt gaagatgcta agaaaaatct
     8821 aatagtagtg ccaatagttg ggactacaat tcctcacgaa gtacgcggac actttggtgc
     8881 tggaaatata ttaataatgc ctgctgtgga aggtacagga gtaatagctg ggggacctgc
     8941 tagagccgta cttgagctag ctggactaaa agacgttaga gctaaatctt tagggtctaa
     9001 caatccaaga aatatggtaa atgctacaat agaaggactt aattctctaa aaacagttga
     9061 agatatagct aaacttagag gtaaaaaagt tgaagaactt ctagggtaag gaggtagtgt
     9121 aaaatggcta aattacaaat aaagttagtt agaagtgtta taggaactac tcctaaccaa
     9181 aaaaagaatg ttgaagcgtt aggattaaga aaaagagaac aagttgttgt aaaagaagac
     9241 aatgctcaaa caagaggtat gataaataaa gtaagtcact tattagaagt aactgaaata
     9301 gctgaataat ttttataaag tagtgcaagg aggtgcaatc catgaagtta catgagttaa
     9361 aacctgctga aggtgcagta agagctaaga gaagattagg tagaggtact gcaactggac
     9421 aaggtaaaac tgcaggacgt ggacaaaaag gtcaatggtc tcgttctggt ggtggagtaa
     9481 gagtaggatt tgaaggtgga caaatgccac tagctagaag acttcctaag agaggtttca
     9541 ataacatatt taagaaagtt tacacagaag ttaatgttga agttttaaac agatttgaaa
     9601 atggaacaga aataactgca gaattattaa aatctactaa aactataagc aaaataggta
     9661 aagatggaat taaaatctta ggtgaaggta atttagagaa agctttaact gttaaagctg
     9721 ctaaatttac agcttcagct caagaaaaaa tagaaaaagc tggtggaaaa gcagagttag
     9781 tataataacc tgctaatctc ctagccagta caggcggggt gaattagcgt gctgtcaaaa
     9841 ttaaaacaag cttggaagat taaagcagta agaaaaaaag taatgtatac acttatgatg
     9901 atcgtgatat tcagaatagg tactactata ccagttccag gcatagacac tagtattata
     9961 caaaagatgg taggcggcaa tagtctactt tctctgtata atatgtttac tggtggagct
    10021 tttagtaatt tttcactatt tgctttaggt ataagtccat atatcactgc atcaatcata
    10081 atacaacttc taacagtagg ttttgaaagt ttagcagaac ttcaaaaatc tggtgaagaa
    10141 ggaaagaaaa aaatcaataa gtacactaaa tatacagcac tagctctagc agttgtacaa
    10201 gctttgggaa ttacattagg aattgtaaga agtgctttaa tatcaaatag tgtgtttttt
    10261 atcacaactg ttgtaataac attagtttca gcaagtatgt tagtaatgtg gattggggac
    10321 aaaattactg aaaaaggaat aggtaatggt agttcagtaa taatatttgc aggtataata
    10381 tcaagaatac ctacagacgt tattaaaatc tcgcaacaag ttaaatctgg ccaagttgca
    10441 ccttgggtta tagtaattct agccgtagtt atattactta cagtaacagg agttacattt
    10501 atacaagaag caactagaaa gatacctgta caatatgcta aaagagttgt tggaagaaaa
    10561 atgtatggcg gacagagttc tcatatacca atgaaagtta accagtcagg agttatgcct
    10621 ataatttttg ctagttcact tttagctttc cctcaaacaa tagctatgtt tatgggtcca
    10681 aatgctcagg cttttgttca aaaatactta agtatggcaa cagaacaagg attttggaca
    10741 tatagatcaa ttgagattct acttataatt ttcttctcat atttttatac gactgtatca
    10801 tttaatacag aagacatatc taaaaatatg aagaataatg gtggatttat accaggtata
    10861 agaccaggtg aacctactat ggattattta aatagaattt taactagatt aactttagct
    10921 ggagctactt tcttagcaat aattgctatg gtgccagctc ttacaacaca ttatatgaaa
    10981 gtaaatatga gtctagctgg gacttcattg ctaatcgttg ttggagttgc tttggagctt
    11041 aagagacagt tagagtcaaa cttagttatg agaagctatc aaggtttctt aaaataaaat
    11101 ggagatggat aatatgagaa taatattact tggacctcca ggtgctggta aaggtactca
    11161 ggcggcagga atagtagaaa aatacaatat acctcatata tcaacaggag atatattcag
    11221 aaagaatata aaagagggaa cagaacttgg aaaaaaagct aaagaataca tggaccaagg
    11281 tttattagta ccagatgagt taactgtagg tttagttact gatagaatat ctcaagaaga
    11341 ttgtaaaaat ggatttatgt tagatggatt tccaagaaat gtagcacaag gagaacattt
    11401 agatatcttc ttaaaaaatg ctggtatatc actagataaa gttgtcaata ttgaagttga
    11461 taagagtata ctagtgtcta gagcagttgg tagaagaata tgtaagtctt gtggagctac
    11521 ttaccatgtt gagtttaatc ctcctaaagt agaaggtgta tgtgatgtat gccaaggaga
    11581 attatatcaa agagctgatg ataatgaaga aactgtatct aagagaatac aagtttatct
    11641 agatgaaact aagcctctag taaactatta tagcaagcaa ggtataatag cagatataaa
    11701 aggtgatcaa gcaatagata aagtatttga agatattgtc acagccttag gaagtggaaa
    11761 ataaatgatt atcttgaaat caaaaaaaga aatagaactt ttaagagaag caggcaaaat
    11821 agttgctgac actcatgaag ttttaagaaa agctatttct ccgggaatat ctactttaga
    11881 attagataaa atagctgaag agaatataag aaaatacaat gcagaacctt cttttaaagg
    11941 atatggaggt tttccaggaa gtatatgtgc ttctatcaat agagaggtag tacatggtat
    12001 tccaggagaa actatattac aagaagggga cattgtaagt ttggatatag gagcctatta
    12061 taaaggatat catggtgatt ctgcgaaaac tcatggagta ggcatgatat ctgaagaaga
    12121 taggaaattg atagaagtaa ctagagaaag cttctatgaa ggaataaaat ttgctaaatt
    12181 aggatataga ctttcagata tctcacacgc agtacaaaca cacgttgaga aacatggttt
    12241 ctcggtagtt agagatttag tgggtcatgg agttggagca aatcttcatg aggacccaca
    12301 agtacctaac tatggactcc caggaaaagg accaagactt agagaaggaa tggttattgc
    12361 tatagagcca atggttaatg caggtcgtta tcatgtaaag actctatcag atgggtggac
    12421 gactgttaca atagatggaa aaaaatcagc tcattatgag catacgatag caattactga
    12481 acatgaacct ttgatattga caaagttgta atttaaaaag taggtgaaaa aagtgctatc
    12541 agataattta agtataggtc aagttgttaa agtttcatta ggacgagata aaggaaattt
    12601 attctttgta gtcaaaataa ttaataatga atatgtatta atagctgatg gaaaaaagag
    12661 aaaacttgac aaacctaagt taaagaaagt gaagcactta aaaaaatata attttattaa
    12721 tgatgaagtt agaaaaagag tagtatctgg acaagagatt acagattctt ttttaagagc
    12781 tgaacttact aagttaaatt agataattta acttattgaa atggagggct ggttagttaa
    12841 tggccaaaaa agatgttata gaattagaag gtacagtttc agaagcttta cctaatgcta
    12901 tgttcaaagt taaactagaa aatggacatg agatattatg ccacatttct ggaaagctaa
    12961 gaatgaactt cataagaatt cttgaaggtg ataaggtgaa tgttgaactt tctccgtatg
    13021 accttacaag aggaagaatt acttggcgta agaagtagac tatattaata tttagtctaa
    13081 ggagggatta ataatgaagg ttagaccatc agtaaaacca atatgtgaaa aatgtaaagt
    13141 aataaaaaga aaaggaaaag taatggttat ctgtgaaaat ccaaagcata agcaaaagca
    13201 aggataagaa attattacta gcattttgtt aaattttata gtataatact atattgtgat
    13261 gttaacaaaa gaacgttaaa atcgttgaac aatctggcgt aggattggca tatgatgtga
    13321 agttttaaac actaaaaact atgtgtagga ggtgcgatca tggcaagaat agctggggta
    13381 gatttaccta gagaaaaaag agcggagata ggcttaactt atatatatgg tataggtaaa
    13441 gcgactgcta acgaaatatt agctaaggct gagataaatc cagatactag aataaaagat
    13501 ttatcagaag atcaagttaa tgagttaaga aaagttatag atgacgattt cttagttgaa
    13561 ggtgacttaa gaagagaaat tgctttaaac ataaagagat taagagatat caaatgttat
    13621 agaggaataa gacacgctaa aggtcttcca ttaagaggac aaagaactaa gactaatgct
    13681 agaactagaa aaggtcctag aaaaacagta tctcgtaaaa agaaaaaata aataaataat
    13741 aactagatga aggagggaaa aggaaaatgg ctaaaccaaa aaagaaagtt acacgtatta
    13801 gaagaagaga acgtaaaaat atagaacgtg gtcatgctca tatacaatca actttcaata
    13861 atacaataat aactttaact gacgttcacg gaaatgctat atcttgggca agttctggac
    13921 aattaggatt caaaggatca agaaaatcaa ctccatttgc atctcaaatg gctgctgaaa
    13981 cagctgcaaa agctgcgatg gaacacggac taaaaagtgt tgaggtattc gtaaaggggc
    14041 caggttcagg aagagaagct gcaataagag ctttacaagc aactggacta gaagtaacta
    14101 tgataaaaga tgttactcca atcccacata acggatgcag accaccaaag agaagaagag
    14161 tgtaattaat aagagtacaa taggaggtgt agataaatgg caagatatac aggtgcatca
    14221 tgtagacaat gccgtagaga gggaatgaaa ttattcctta aaggtgatag atgttataca
    14281 gataaatgtg ctatagtaaa aagaaactat gctccaggtc aacatggcca aggaagaaag
    14341 aaagtttcta actatggatt acaattaaga gaaaaacaaa aagttaaaag aatatatgga
    14401 gttttagaga ctcaatttag aaatttatat gaacgtgctg aaaatatgcc tggtaaagca
    14461 ggtgagaact tattaagttt attagagaga agattagata atgtagttta cagaatggga
    14521 ctagcatctt ctagaaaaga agctagacaa ttagtgactc atggtcattt cactttaaat
    14581 ggaaataaag tagacatacc ttcattaata gttaaggttg gagatgttat agaagttaaa
    14641 gagaaatcaa gatcttctgc aaaattcaaa aatttagtag aagttaactc aagaatagct
    14701 cctaagtggt tagaagctaa tgtagaagga atgacagcaa aagtagttgg tgttccaaca
    14761 agagaagata tagatcttga gatagcagag cacttaatca tagaacttta ctctaaataa
    14821 taagcacact tttaaaaatt attttaaaat tttgtttacc ctcagtggat tactgaattt
    14881 taaggagggt tttgtccatg atagaaatag aaaagccaaa agtagatata gttgagctta
    14941 gcgaagacta tagatatggt aaatttgtta tagagcctct agaaagagga tatggaataa
    15001 ctataggtaa cgcattaaga agaatattat tatcatcact accaggtgta gcagtgaacg
    15061 ctataaaaat agatggagtt cttcatgaat tttcaacaat acctggtgtc aaagaagatg
    15121 ttactgagat aatattgact ttaaaagagc tttcagcaac tatagatggt gaaggaagta
    15181 gaacacttaa aatagaagct caaggaccat gctctatcac aggtgcagat ataatctgtt
    15241 ctccagatgt agaaatatta agtaaagatt tagcaatagc tacattagat gataatgcta
    15301 agcttaatat ggagatattt gtagataaag gtagaggtta tgtttctgct gaagaaaata
    15361 aaacagagaa tgttccaata ggtgttttac ctgtggattc aatatatact cctgttgaga
    15421 aggttagtta tcatgtggaa aacacaagag ttggtcaaaa aacagattat gataaattag
    15481 tactagaagt ttggactaat ggtagtataa atcctcaaga gggtatatca ttagctgcaa
    15541 aggtactagt tgagcattta aacttattta tagacttaac tgaacatgta agtagtgttg
    15601 aaatcatggt agaaaaagaa gaagatcaaa aagaaaaagt tcttgaaatg actatagaag
    15661 aattagattt atcagttaga tcatataact gtttaaagag agcgggaatt aatacagttg
    15721 aagaattagc taataaatct gaggatgata tgatgaaggt tagaaaccta ggtaagaagt
    15781 cattagaaga ggtaatacaa aaactagaag aacttggatt gggattaaaa ccaagtgaag
    15841 aatagcgagc aaaggaggga taagaatggc taagtaccgt aaattaggac gtgaaacagc
    15901 tcacagaaac cttatgttaa gaaacttagt aacttgttta ctaagaagtg gaagaataga
    15961 aactacagtg actagagcga aagaaactcg tagaatggct gaaaagatga taactcttgc
    16021 taagagagga gatcttcatg ctagaagaca agttttagct tatgttatgg atgaaacagt
    16081 agttaataac ttattcacag atttagctcc aaaatatgct gagagaaatg gtggatacac
    16141 tagaataata aaaatagggc caagaaaagg cgatgctgct gaaatggctt ttatagaatt
    16201 agtatagtaa ggaaaggatt aggcttgatg gcttaatctt ttttttattt ttttgactaa
    16261 ggtatgatat aataatgtgt aaaatgattt ttataagtta ttgcttacta aaaggagtat
    16321 gaattaatgg ataatatagt aaaggtaaat aatatttcgt ttgagtatat tacagatgaa
    16381 gcaaaactta aagcaataga taatttaagc ctagatgtta aaaagggaga atttgttgcg
    16441 ataataggac ataatggttc tggtaagtct actttatcta aaaacttaaa tgctattctt
    16501 atgcctactg aaggaaatat tcttattgat ggtatgaata ctaaagaaga agaaagatta
    16561 tgggatataa gacaaactgc aggcatggta tttcaaaatc cagacaatca aatagttgct
    16621 actatagtag aagaagatgt tgcatttgga cctgagaact taggtataga accaaaagaa
    16681 ataagacgaa ttgtagagga gtctttaaaa agtgtaggta tgtatgattt aagagataga
    16741 cagcctcact tgttatcagg aggtcaaaag caaagagttg caatagcagg aattattgct
    16801 atgaggccta aatgtataat atttgatgaa gcaacagcaa tgttagatcc atctggaaga
    16861 aaagaagtta tgaaaacaat aaaaagactt aataaggaag aaaatataac tgttatacat
    16921 ataacacact ttatggaaga agcagtagaa gctgatagag ttgtagttat ggaaaaaggt
    16981 aagaaaatct tagagggaac tcctagagaa gtatttagta aaataaagat gttaaaggaa
    17041 atcggtttag atgtcccttg tatgacagag ttatctagtt tattaataga agaaggtata
    17101 aatattagtg gtgatatatt aactgtggat gagatggtga tggaattatg tcgattatag
    17161 taaagaactt aacacatata tataatgaag ggatgccttt tgcaagtaag gcacttgatg
    17221 atatttcttt tgagattaaa gatagagatt ttgttggtct cataggtcat acaggctcag
    17281 gaaaatctac attaatacaa catttaaatg gattactaaa gccttcctca ggagaaatat
    17341 ttataaatga ttttaatata acagataaaa acttgaattt aactgagatt agaaaaagag
    17401 taggggtcgt atttcaatat ccagaatatc aattgtttga agaaacgatt gataaagata
    17461 tagcctttgg accctctaat ttgggcttag aggagtccga gatacataat agggtaaaag
    17521 catcgatgga agctgtagga cttgattatg aggaatttaa agataaatct ccttttgaat
    17581 tatctggagg acaaaagcgt agagtagcaa ttgctggagt tatagccatg aacccagaag
    17641 ttctcatatt ggatgaacca actgctggtc ttgaccctgg tggaagagat gaaattttta
    17701 acttaataaa aagcttacat gaaaaaaaga atatgactat aatactatca tcacatagta
    17761 tggatgatat ggcaaaatta gcaaaaactt taattgttat gaatcatggt agtgtagaat
    17821 ttataggaac tcctagagaa gtatttaaat caaacgctag taaattaaaa gatataggat
    17881 tagatattcc acaagtttta gagcttgcat tgaaattgag agaaaaaggc tttgacatca
    17941 gtgaggatat cttgacttta gaagaagcca aacaggaaat attaaaagtt gtgagaggac
    18001 gaggattatg ttaaaagata taactatagg gcaatactac ccaacaagtt ctgctattca
    18061 taaattagat ccaagaataa agcttgtagc aacaattgta tttatgatat ctatatttgt
    18121 agtaaataaa ttttggcctt atatagtagt tttattatgt ttattagcta tgataaaatt
    18181 ggctaatata ccaatcaaat atattgttaa gggtgttaaa cctcttaagt ggattatttt
    18241 gtttacattt tttataaata tttttttctt accaggtgat gaaatatggt catttggatt
    18301 tttaactatt acaagccagg gtctaagaca agcaattttt atggcaataa gattaatatt
    18361 tttagtagtt ggtacatcat tgcttacttt aacaacttca ccaatagaat taactgatgg
    18421 aatagagaga ttgttgaacc catttaagaa aattggtttg ccagttcatg aacttgcaat
    18481 gatgatgaca attgctttac gttttattcc tacgttatta gatgagacag ataaaataat
    18541 gaaagctcag atgtctagag gtgcagattt tgaaagtaag aatcttataa atagagcaaa
    18601 gaatttagta ccacttttgg tacctttatt tgtgagtgca tttagaaggg ctgatgaatt
    18661 ggctatggcc atggaggcca gatgttacag aggtggacat aatagaacta agatgagaga
    18721 gtctgttata tcaagaagag attatatggc atgtgtattt caggttgtct atctaggagc
    18781 aattatagct actagattta tagcaattta attaataagt tatagatata aatgaggtaa
    18841 aatatgagaa atataaaaat tacaatccaa tacaatggta agaattattg tggatggcaa
    18901 aaacaacttg attctttagg gatacagggt acaattgaaa gggctatata tgatataaca
    18961 aaagaaaaag taagtcttat agggtctggg agaactgatt caggggttca tgctattggt
    19021 cagattgcaa actttaaaac taattcaagt ataagtatag aaagtatacc aatggcacta
    19081 aatgccaagt taccaaaaga catatcagtt atagatgcat gtgaagtaaa tgatgatttt
    19141 cactcaagat atagtgcaaa aggtaaaaca tataaatatt tagtatataa tagcaagttt
    19201 agaaatccaa tacttagtga gatatcatat caagtaaaat atgaacttga ttttgataaa
    19261 atgtgttcag aagcaaaact tttattagga acacatgatt ttaaaggctt tatgagttct
    19321 ggctcgtctg taaaagatac agttagaacg atatatgata ttgatataat tagaaaagat
    19381 gacttgatta cttttgagat tagtggtaat ggttttttat ataacatggt gaggattatt
    19441 gttggaacac ttgttgatat aggtcgtggt agaattaacg aaccattttt agatataata
    19501 caatcaaaaa ctaggtcaag atgtggacat acagctccag cacaaggatt atttctcaaa
    19561 aaagttcatt attaacttga cacaccaggc tacatgtatt aaaatatatg agaatgtgtt
    19621 aataagtcca cttgccccgg acttgacata aacggtaaaa aaattttatg aaaagtagtt
    19681 aaggagggac agttatgaaa agttatatag ctaagccagc tgatgtacaa agaaaatggt
    19741 acttagttga tgctgaagga aaaacattag gtcgtttagc aacagaaatt gcgacagtat
    19801 tgagaggtaa acataaacca acatttactc ctcacgttga tggtggagat tttgttgttg
    19861 tagtaaatgc agaaaaaata gttttaagtg gtaagaaatt agatcaaaaa tactacagat
    19921 accatactgg ttatgtaggt ggattaaaag aaatatctta cagagatatg atggataaga
    19981 aaccagagga agttatatca catgctgtaa gcggtatgtt acctaaaaat aaattaagaa
    20041 gtagaatgat gactagatta agagtatttg caggtgctga gcacactcat gcagctcaaa
    20101 atccagaagt tttaaacttt aagtaagatt aataggtgaa aggaggagtt tataatggct
    20161 aacgttcaat attatggaac tggaagaaga aaaagttctg ttgctagagt aagactagtt
    20221 gcaggtgaag gaaatatatt agtaaatgga agagcattag aaaattattt taactatgag
    20281 acattaataa gagatgttaa acaaccatta gttttaactg gtaatgaaaa taaatatgat
    20341 gttatagtaa aagttgaagg tggaggattc actggacaag ctggagctat aagacatggt
    20401 atatctagag ctttattaaa agctgattta gatttaagac ctgctttaaa gaaagaaggt
    20461 ttcttaacta gagatgcaag aatgaaggaa agaaagaaat acggattaaa agcagcaaga
    20521 agagctccac aattctcaaa aagataatta cttatttttt tggaaaaaca aaatccttta
    20581 tttactgcta ttcagaaaat attggtattt taaaaatgtt gggttaagtt aattattaat
    20641 atataataaa ataataagat atcttattag gatgtttttc attaagaaaa gcatcctttt
    20701 tattttgact atctaataaa gaatatatgc taataatatt tagattttat ttttataata
    20761 ttgtgattta aaagtttaca taagatgata tattttaaga tattatttaa caatataaat
    20821 tatgaatata tcttatcaaa gtttataata taaactaaat ataatttatt gtatagtatt
    20881 gaatgttaga taaatatagt gagttaaaga ggggtaggga catagtatca ttacataata
    20941 atgtattaat actaataaat agatatatat aaatattaaa agaaaattta aatgtttgta
    21001 ctaatatttg gaatattata gtgtttttat aagtatacat atacagggtg atgttgtgag
    21061 aaagtacata aaacatataa tttttagttt tgcaatgata tgtctagtag tagtatccat
    21121 atttgaaatt aaaaatattt ctgaagatgt tatcaagtat atgccagtaa caaacaaaac
    21181 tataattctc gatgctggtc atggaggtat tgatccaggc gcattaaata aggataagag
    21241 tacatctgaa aaggatatca acctagcaat aacacttaag cttagagagc ttatagaatc
    21301 aagtggtggt cttgtaatat taactcgaga ggatgacagt agcctttata aagaagaaaa
    21361 taataaaact acaagacaaa aatataatga gaatttgaag aatagaaaag aaataatatc
    21421 taattctaat gcaaatatgt ttgtctctat acatcttaat gcatttgagc aatcaaaata
    21481 ctatggagct caaacttttt atcctaaaga taagcaagat agtaaagaat tatcgaagtg
    21541 tattcaagaa gaacttaaaa gagtggtaga taaaacaaac aatagagaag tcaaaccgag
    21601 ggatgatata tatcttttga aagaaaataa tattccatca gtattgatag aatgtggttt
    21661 tttatcaaat gaaaaagaga gtaaactttt aactgatgaa acatatcaag aaaaaatagc
    21721 atgggcaatt tacataggaa tacaaaaata tttaagttga aataactata aaaataggtc
    21781 taaatatgta aatagcttgt attaaagcag ttaaaatgca ttaatatagg ctatttttat
    21841 tttgacaaaa aaatatttaa aataaaagtt aaaaaatagt tgacttagaa tggtatagat
    21901 gatattatat atgagtgccc aaaaggagca ccaaaataag ac
//
LOCUS       DAMJVM010000056        21777 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.058, whole genome shotgun sequence.
ACCESSION   DAMJVM010000056 DAMJVM010000000
VERSION     DAMJVM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21777
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.058"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            95..436
                     /locus_tag="PC523_002294"
     CDS             95..436
                     /locus_tag="PC523_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HDF2475810.1"
                     /translation="MKEENFINIEYEDKRFSINSKEVLYIEKQEYEVKIYTENGNYEV
                     GENAPCIRKLMKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            587..1000
                     /locus_tag="PC523_002295"
     CDS             587..1000
                     /locus_tag="PC523_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HDF2475811.1"
                     /translation="MLELKNNSGTSERKLKYPCIYKHFKGKYYATMGLSKAIDNVENI
                     CEIYSKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFGRPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1200..1841
                     /locus_tag="PC523_002296"
     CDS             1200..1841
                     /locus_tag="PC523_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDF2475812.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILADKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIIKMNDTSHIKNHVKINNSALE"
     gene            1865..3118
                     /locus_tag="PC523_002297"
     CDS             1865..3118
                     /locus_tag="PC523_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2475813.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNRDIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEALNKDLLNHNVKIIYNKKVIDIITEEIAIKDDSNKNKSKYLIKGI
                     ILDNGDKILGDKIIISTGGVSYSKTGSDGLMYKILKNHGHTLNKLYPALVPLTIEEKW
                     IKNLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSSDEISSIIREFPNKNVLNNLKQILPQNFLKEILSLLSLSDKKASDLSK
                     ADEIKIIEYIKNMKLTCTGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3164..3814
                     /locus_tag="PC523_002298"
     CDS             3164..3814
                     /locus_tag="PC523_002298"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDF2475814.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGVDVNNEKEVIQIAENSDIDFKDNSIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVQVQRKIGMKSSVILDGRDIGSYVFPNADYKFFLVATPEERGNRRYKELCDKG
                     YNTTLKAVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVISKINL"
     gene            3865..4461
                     /locus_tag="PC523_002299"
     CDS             3865..4461
                     /locus_tag="PC523_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDF2475815.1"
                     /translation="MNFYKFVINIFKGFTRIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAVENREIAAIAKKELFKVKPLGFILKKMHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLTNDDHERISQEILEVIKQNYFKYSK"
     gene            4474..5313
                     /locus_tag="PC523_002300"
     CDS             4474..5313
                     /locus_tag="PC523_002300"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDF2475816.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKSDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKNKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKKYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVNVCEELVPTFAIETKDELDVNMLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5566..6720
                     /locus_tag="PC523_002301"
     CDS             5566..6720
                     /locus_tag="PC523_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HDF2475817.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDTSKNNSKENKEKHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIILIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMVITTIPAIIVGMIVYAFLG"
     gene            7019..8716
                     /gene="ade"
                     /locus_tag="PC523_002302"
     CDS             7019..8716
                     /gene="ade"
                     /locus_tag="PC523_002302"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDF2475818.1"
                     /translation="MGNLKKQCDVASGREKADIVLKNGTIINVFTEELIVGDVAIVGD
                     TIVGIGDYKGTVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGASAIDFLLESTKDIPLNVYIMIPSSVPATSFETNGIGEFSAKDMESYINN
                     PRILGLGEVMCFNDVINSDNNILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVNGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPIRAIKVATYNSARAYGLKRIGAIGAGYKADIVVLNDLE
                     KMEVDSVYKDGNLVKEEMFSNYNYDIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKLYESLPCVEGYFIPSEEYSKLCVVERHRMTGNIAIAPLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDIIVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTLT
                     AKEVQETTDNMLEIARKMGVPKYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
     gene            8958..9416
                     /gene="bcp"
                     /locus_tag="PC523_002303"
     CDS             8958..9416
                     /gene="bcp"
                     /locus_tag="PC523_002303"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDF2475819.1"
                     /translation="MLSIGTRAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDVQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEK"
     gene            9432..10175
                     /gene="yaaA"
                     /locus_tag="PC523_002304"
     CDS             9432..10175
                     /gene="yaaA"
                     /locus_tag="PC523_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDF2475820.1"
                     /translation="MIIMLSPAKNMKNIEVSDRDLSLPCFIDNTKEIVENINTFSVED
                     FKKKMKINEKLAILNKNRFESIKFDKLGSPAILTYDGIQYKNIEAENFTRQDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQAKLRVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGIFKVESTQAKKARGMMTKYIIKNRIRD
                     IEELKKFNLEGYKYQENLSNNSEYIFVKE"
     gene            10303..12963
                     /locus_tag="PC523_002305"
     CDS             10303..12963
                     /locus_tag="PC523_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDF2475821.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPNRTTLQMLWSQITDAMVLILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKEYVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     NTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMSKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEILEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITELDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGIVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTISYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSALLIAVILFVPVFRNAFNLTILNSL
                     EWLITIALAIMSVVQVEVGKIIKRKKLKNS"
     regulatory      13013..13115
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            13223..14488
                     /locus_tag="PC523_002306"
     CDS             13223..14488
                     /locus_tag="PC523_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HDF2475822.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVASVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENAFKIAKFLEKHENVDWINYPGLEDNKYYENAKKYLSKG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     gene            14518..15408
                     /gene="metA"
                     /locus_tag="PC523_002307"
     CDS             14518..15408
                     /gene="metA"
                     /locus_tag="PC523_002307"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDF2475823.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKKLTYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKSKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKNSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINRGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            15702..16145
                     /locus_tag="PC523_002308"
     CDS             15702..16145
                     /locus_tag="PC523_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2475824.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLTKFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQENISSSIFDNYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            16175..17992
                     /gene="ftsH"
                     /locus_tag="PC523_002309"
     CDS             16175..17992
                     /gene="ftsH"
                     /locus_tag="PC523_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDF2475825.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLNILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALSEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKTCH
                     KNAIEILENNIDKLHELAKYLLEKETITGDEFMEILNLN"
     gene            18170..20872
                     /locus_tag="PC523_002310"
     CDS             18170..20872
                     /locus_tag="PC523_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HDF2475826.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYASGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKKFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     VSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETASKREENTKMIYQIIKGFLRLSNKDDIINKGIELLSLSLSRDVICYLNVDKYNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCVDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVEDNKDKGSTFKFSLPKENE"
     gene            20906..21604
                     /locus_tag="PC523_002311"
     CDS             20906..21604
                     /locus_tag="PC523_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2475827.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEVQQKDDVKSTFEVDKLLIDFDKRKVIVDDIEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
BASE COUNT         8725 a         2352 c         3927 g         6773 t
ORIGIN      
        1 tatattaagt aaatcagtct tacatatatt tccgatagat tatattgata aaaaacacta
       61 aaattaaatt aataaagtac ttggagggat ggaaatgaaa gaggaaaatt ttattaatat
      121 tgaatacgag gataaacgtt tttcaattaa tagtaaagaa gttttgtaca tagaaaagca
      181 agaatatgaa gtaaagattt acacagaaaa tggtaattat gaagttggag aaaatgctcc
      241 ttgtataaga aaactaatga aagaagaaaa ttttttcaaa tgtagtaata gttatattgt
      301 aaatttagat aagtgtgaat tttatctcag caataattgt atttttgtga ataataattt
      361 tataagaatt ttaggttcag aaagaaaaaa gaagttaaaa aagaaattag aaaaattata
      421 tgattatgaa gattaatatt gagaggctgt attttataca gcctctttat ttattcattg
      481 tattattaat aaattttaaa ttatgataaa atataaaaca agtaacttat ttcagaaaaa
      541 aatacttttt aaaagaaagg gacgagatat gggagattat aataaaatgc ttgaattaaa
      601 gaataattca ggtactagtg aaagaaaatt aaaatatcct tgtatctata aacatttcaa
      661 agggaaatat tatgctacaa tgggtcttag caaagctatt gataatgtag aaaacatatg
      721 tgaaatatat agtaaagaaa atttaatcca aaatagaaat aaatataaat tagttattag
      781 acatactgaa agagaagaag atatctatgt ctatagagat ttagatggaa acttttatca
      841 taaaaaagaa gaagatacca atgatttagt tttatacaag actttatatg acgatacagg
      901 aatatttgga agacctttgg atatgttttt agaaaaagta gatacagata agtatgtcaa
      961 ttcaattcaa gagtacagat ttgaagaagt atataaataa tataagagaa atatgtttat
     1021 aaatttagaa aaattattta agagtataat aatttgcaga aatacaaatg agatttattg
     1081 taaacaatat gttagattgg attataaaat agaaacaaag taaagtatta tatgatataa
     1141 ttaagttttg agaagtgata ttatatttaa taaaaaatag gcatatatta ggaggacata
     1201 tgggaaatac tttttatata gtaagacatg gacaaacaga ttggaatata ttaggaaaaa
     1261 cacaagggca tggtaattct gaccttacac ctcaaggtat agaacaggca aaagaacttt
     1321 ctgaagatat tggcaagtat tcaattgatt atatttttag tagtgacctt ggaagagcaa
     1381 tgcaaacagc tcaaatttta gcagataagc tcaatattga agttcaaaaa actgaagcac
     1441 ttagagaaat gggattcgga gtttgggaag gtcttttaat aaaagaaatt caaaaggatt
     1501 attcagatat atatgcaaca tggagaaatg agccacattt agtaaatata ccagaaggcg
     1561 aaacgcttaa aattataaaa gaaagagtag atgcttttat aaaggaatta aatgaaaagt
     1621 acgataataa aaatataatt cttgttacac attcaattac attgagggtt atgttgttgt
     1681 catttttaga atcagggatg gaaaatatat atagaattaa gcaggataac actgctttaa
     1741 acatagtaga atttaaagat tacggacctg ttataataaa aatgaatgat acaagtcata
     1801 taaaaaatca tgtaaaaata aataattctg cattggagta ggaatcaatt ataaggagaa
     1861 atttatgagt aaagttatag tagttggtgg aggagcttct ggaatgatgg cagctttgtc
     1921 agcttcaaaa aataataatg aagtaatatt agttgagaga aatggagaat taggaagaaa
     1981 actgagagca actggtggag gtagatgcaa ttttaccaat aacagagata tagaagattt
     2041 ttttgataaa gtcgttagca ataaaaaatt tctctatagt agtttctata catttacaaa
     2101 taaagacctt atttcatatt ttgaaagtag aaatttggaa tataagattg aagaagaaaa
     2161 tgaccacaaa gtatatacta aaaatgataa atctatagag gtaatagaag cattaaataa
     2221 agatttactc aatcacaatg taaaaataat atataataaa aaagttatag atattataac
     2281 tgaagaaatt gctataaaag atgattctaa taagaacaag tcaaagtatc taataaaagg
     2341 gattatttta gataatggag acaagatact aggagacaag attattatat caactggagg
     2401 agtgagttat tcaaaaacgg gttcagatgg tttaatgtac aagattctta aaaatcatgg
     2461 acatacatta aataaactat atcctgcttt agttccactt acaatagagg aaaaatggat
     2521 taaaaatttg caaggaatat ccatgaagaa tgtggaaata tcttgtaaga ttaagaaaag
     2581 gaaaatatca aaatcgggag acatgttatt tgcacatttt ggaataacag gaccttgtgt
     2641 gcttataatg tcttcttata ttaataagat tattgaaaaa gaaaaggtag aattaaatat
     2701 agatttttta ccaaatttaa gtagtgatga aatttctagt ataatcagag aatttccaaa
     2761 taaaaatgtt ctaaataatc ttaagcaaat actacctcaa aatttcttaa aagaaattct
     2821 atctctatta tctttgagtg ataaaaaggc aagtgatttg tctaaggcag atgaaattaa
     2881 aattattgag tatataaaga atatgaagct aacttgcact ggaactacag gtataaacac
     2941 tggtatggtt acttctggtg gtatttcagt taaagaaatt aattcttcta ctatggaatc
     3001 taaattagtt aagaacttat tttttacagg ggaagtaatt gatatagatg cagaaactgg
     3061 tggatacaat ttacaaatag cattttcaac tggatatctt gcgggtatta gtgtataaat
     3121 ataatttttt aataattaaa tatgaataaa tagaggtgta tatatgggta atttagttat
     3181 agctgtagat ggtccagcag gagctggaaa aagtacaata gccaagatag tagcaaaaaa
     3241 gctaaatata aattatatag atacaggtgc tatgtacaga gcagttactt ataagtgttt
     3301 aaaaagtggg gttgatgtca ataatgaaaa agaagttatt caaatagctg aaaattcgga
     3361 tatagatttc aaagataata gtatatattt ggacaaagaa gttataaatg aagaaataag
     3421 aacgatagaa gtaagtaata atgtttctaa tgtggcaaaa ataaaagaag taagacagtt
     3481 gatggttcaa gttcaacgaa aaataggaat gaaaagttca gtaatactag atggtagaga
     3541 tataggttca tatgttttcc caaatgcaga ttacaaattc tttttagtag ctactccaga
     3601 agaaagagga aatagacgct ataaagagct ttgtgataaa ggatataata ctactttaaa
     3661 agcagtaatt gaagatataa taagacgaga tgaaattgat tcaaatagag aatttgcacc
     3721 attagtaaaa gctaatgatg cactagaaat agatactaca ggaaaaacca tagaagaagt
     3781 tgtagaagaa gttatatcta aaataaatct ttagtttttt atgtattata ataaaaataa
     3841 atgtagtgtt gaagggagat aaacgtgaat ttttataaat ttgtaataaa tatttttaaa
     3901 ggttttacta gaatattttt taaatatgaa gttataggtg cagaaaatat tccagataga
     3961 gggaatatag taatagcatc aaaccataaa tcgaatctgg accctatatt cttagctgct
     4021 gccgtagaaa atagagaaat agctgcaatt gcaaagaaag aattgtttaa agtaaaacca
     4081 ttaggattta tattaaaaaa aatgcatgtt atgcctataa atagagaaaa gccagatgta
     4141 tctacaataa aaactatttt aaggtcagtt agagatggtt atgtgttagg catatttcca
     4201 gagggtacta gaataaaggg agattctttt ggtaaggcta aggcaggtct ttctgtattt
     4261 acaataaaaa gtaaatctaa agttgttcca gtatcaataa tatccaaata caaacttttt
     4321 agtaaagtaa ttgtatatat tggagaacca atatcatttg aagaacattt taaggaaaaa
     4381 ttgactaatg atgaccatga aaggatatca caggagattt tagaggtcat aaaacaaaat
     4441 tactttaaat attcaaaata agggggtaaa tttatgaaca taaaaatagc aaaaaatgct
     4501 ggattttgtt ttggagtaaa aagagcaatg aaaatggcat gggatgaagt tgaaaaaagt
     4561 gattctggca tatatgcttt aggtcctctt attcataaca agcaagcagt agcaaaatat
     4621 gaagaaaaag gtcttaagac tgtgaatgaa attgatacaa taccaaatca tgaaaatatg
     4681 ataataaggt ctcatggagt tcctgaaaat atatataaag aagctaagaa caaaaaatta
     4741 aaaattgtgg acactacatg tccatttgta aagaaaatac atacagtagt aagtgaatat
     4801 cataataaag gctatgagat aatagttatt ggagatatga aacatccaga agttataggt
     4861 ataaatggat ggtgtgaaaa ttcagctata ataataaaga ctttagaaca aatggaaaat
     4921 atggaatttg ataactcaaa aaaatattgc cttgttgcac agactacaat aaatccagag
     4981 ttgtatataa gtatagttaa taaactatca gataaattag aagaaatagt atttaatgat
     5041 acaatttgtt cagctacaaa aacaagacaa gagtcagcta aggagttagc taaagaagtt
     5101 gattgtatga tagttatagg aggcaaacac agctcaaata ctcagaagtt agtaaatgtt
     5161 tgtgaagagt tagtaccaac ttttgcaata gagactaagg atgaattgga tgtaaatatg
     5221 cttaaaaaat ataaaaattt aggtattaca gcaggagcat ctacacctaa ttggataata
     5281 gaagaagttg taacattcct tgaaaattta taaatttgat agataataaa aataggtgga
     5341 tattattata acataaaact tattataata tctgcctttg atttcatatt acatgtggca
     5401 aatagataac ctaatattaa aaagtgtgta tcttaagatg aaaaactaat agtataagat
     5461 gcgcactttt taatattaga aatttaaaat aaatattgat attgattatc aatagaaaat
     5521 ggaatataat ataattattg caaaattagt ctaggaggta aattaatgga aatgttaata
     5581 tccgcttctg aagaggcgtt tttacatgta ggttctatga ttggattttt tattttgtta
     5641 tttggatata ttaattataa aactagtgga aattttacta atattatatc aaaaaacaga
     5701 aaatttcaac ctttaatagg tgctttaatt ggagctatac caggttgtgg gggaagtttg
     5761 gcaataatgc cattgtatat aaatggaaaa ttaagctttg gagcaataat tgcaagttta
     5821 atagctagta tgggagacgc tgcatttgta cttatttcat caaatattaa aatgtatttt
     5881 tttgtcacta tagttagtac tattacagga ataataactg gacaattagt ggattacttt
     5941 aaactagaag aaaaattagg tctaaaaaac cgtagaaaat cggataaata cgatacaagt
     6001 aaaaataact ctaaagaaaa taaagaaaaa cataatcatg atgaaataat tttggatact
     6061 ctggctaaat ctcatggaaa tacaaataga cttgcattca taataacaca tggaaaggga
     6121 tataaaattt atataggaat tatattaatc ggttttatat ttatgtcact tgctcactct
     6181 ggacttaatc ttcctataat tgagaagtta cattcattgg aagaagtaat agcagtgatt
     6241 ggaatattat tttcaataat ttatatgtgg tgttttaaaa aggtatttaa aaattctaat
     6301 cagaaagagg aagaaaataa aaagatttct ttacgagaaa tgctgataca ttctgttggc
     6361 gaaatatcat ttgtaattac atggattttt attgcatacc ttatttacga tttgataata
     6421 ttaattttag gtggagatga atatttagtt aaactagtat tatcaacagg tgtaataagt
     6481 gtatttatag gtgcaggtct tgggcttatt ccaggttgtg gtatacagat tgtacttatg
     6541 tcattttatt tgaagggaac tataccactt ggggcagtga tagccaactc tatttctcaa
     6601 gatggagatg cacttttccc acttttagca atggataaaa aatcttcttt atgggctatg
     6661 gttattacta ctataccagc aataattgta ggaatgatag tttatgcatt tttaggttaa
     6721 atataaataa aaaagtatta aaaaattaat aaatcacata aatttatttg caagaatagt
     6781 aaacttaaat atataaataa aaaattcaat ataacttaaa ttattactta aatattaatg
     6841 agaacatggg agataatatt attccttatg ttctctctat ttaattaaat gaattatgga
     6901 acaaatttca agtgatactt tttattaaaa ctcatatata atatatatta ataaattaaa
     6961 ctataatatg taatacagcc agtatattga tataaaaaac ttaagaggag ttgtaaaaat
     7021 gggtaattta aaaaaacagt gtgatgttgc aagtggaaga gaaaaagctg acattgtact
     7081 taaaaatggg acaatcataa acgtatttac agaagagcta atagtaggag atgtagcaat
     7141 agtaggagat actattgttg gtattggcga ttacaaaggt actgtagaaa ttgattgtag
     7201 caacaaatat atatcacctg gttttattga tgcacatatg catattgaat ctacaatggt
     7261 aatgccaatt gaattatcaa aaaaattatt aaaatcaggt acgacaacca tcatagccga
     7321 cccacatgag ttggtaaatg taaaaggagc tagtgcaata gattttttat tagaatcgac
     7381 aaaagatata ccacttaatg tatatattat gataccttca tccgtacctg caacaagttt
     7441 tgaaactaat ggaattggtg aatttagtgc aaaagacatg gagagttaca taaataatcc
     7501 tagaatttta ggcttaggag aggtaatgtg ctttaatgat gttataaatt ctgataataa
     7561 tatcttggat aaactagagc tttttaaaaa taaagttgta gacggacatg cacctaatat
     7621 aaatggtaaa tcgcttcaga catatgtatg cgcaggaata gaaaatgacc atgaatgtat
     7681 aacttttgat gaagtatatg aaaaattgag agcaggttta aaaatattaa taagagaagg
     7741 aagtgcagca aaaaatttaa aatctatagt aaatggtatg ctaaagcata acttgccaat
     7801 tgaagagttt atgttttgca cagatgataa gcacttagat gatatagaaa aacaaggtca
     7861 cataagatgg aatataaaat gtgcgattga tttaggtatg gaacctatta gagctataaa
     7921 agttgcaact tataattctg caagagcata tgggctaaaa agaattggag caataggtgc
     7981 tggttataag gcagatattg ttgtattgaa tgatttagaa aaaatggaag tggatagtgt
     8041 atataaagat ggtaatttgg taaaggaaga gatgttttct aattacaatt atgacattaa
     8101 agataaagaa ttgcttaata cagtcaaatt taaatatatt aataaggaaa aaatacagtt
     8161 aaaagtaagt gaaaaaaatt atgtaatgga gattgtacct tatcaaattt tgacaaataa
     8221 gttatatgaa tcattaccat gtgtagaggg atactttatt ccaagtgaag agtattcaaa
     8281 actttgtgtt gttgagagac atagaatgac tggaaatata gcaattgctc cactaaaagg
     8341 atttggcata aaaaatggag caattgctac tactgtggct catgattctc ataatattat
     8401 agttgcaggg gataacgatg atgacattat agttgccata aattacttaa aggaaataca
     8461 gggtggttat gtaattgtat caaatggaaa agttcttgca cacctaagtc ttcaagtggc
     8521 tggattaatt agtactctta cagccaaaga agtacaagaa actactgata atatgttgga
     8581 gatagcaaga aaaatgggag ttccaaagta cgtagaccct tttattacac tttcttttat
     8641 ggcacttcca gttgttccac aaataagact tacagattta ggtctatttg atgttgagga
     8701 atttaaattt atataggcac cataataata aattaggata ttcaaaaata gagattgtac
     8761 tacaccccaa ttgttggact aaaaacctaa tgattggggt gtttatatat aactaaatac
     8821 aatgcatata aatacatgaa aattatatat ttatgaaact ttatacataa aaaataaaat
     8881 attttattta tatatagatt tatagggtaa taatataagc atagtataga attatcaata
     8941 aaaaaggaga gatatttatg ttgagtatag gaacaagagc accagaattt acactagaag
     9001 acaaagatgg aaataaggta agtatgtcag attttaaagg taaaaaagta gtagtatatt
     9061 tttatcctaa agataataca ccaggatgta cacgtcaagc ttgtgctttt agaaatgcgt
     9121 atgatggatt taaaaaggag gatgtacaag ttattggtat aagtaaagat agtataaaat
     9181 cacatcaaaa atttgcagaa aagcatgaac ttccttttat tttactttct gaccctgatt
     9241 tagtcgctat aaaagccttt gatgtatgga aagagaaaaa aatgtatgga aaaactgcat
     9301 tgggagttgt acgtgcgaca tatatcatag atgaaaatgg aattattgaa aaagtttttg
     9361 aaaaagcaaa accagacaca aatgcacaag agattcttga atatttggaa aaataggagc
     9421 aataagaatt tatgattata atgttatcac cagctaaaaa tatgaagaat atagaagtgt
     9481 ctgatagaga tttgagtttg ccctgtttta tagataatac aaaagaaata gtggaaaata
     9541 taaatacatt tagtgtagaa gattttaaaa agaaaatgaa aattaatgaa aaattagcta
     9601 tactaaataa aaatagattt gaaagtatta agttcgataa attaggaagt cctgcaatct
     9661 taacttatga tggaatacaa tataagaata tagaagcaga aaattttaca aggcaagatg
     9721 aagagtttgc aaatagttgt attcgaatca taagtggatt gtatggagtt gtaaaaccat
     9781 atgatagtat atatgaatat agattagaaa tgcaggcaaa attacgagta ggtgagttta
     9841 agaatttata cgaatattgg ggaaatcgta tatataaaga actaattaaa gaaaaaacag
     9901 ctattgtaaa tctttcatca aatgaataca gcaagtccat agaaaaattt attaaagata
     9961 gtgatacata tattacatgt acttttaaag taaataaaaa tggtatattt aaagtggaat
    10021 ctacacaagc aaagaaagct agaggtatga tgacaaagta cattataaaa aatcggataa
    10081 gagacattga agaactgaag aagtttaatt tagaagggta taagtatcaa gaaaacttat
    10141 ctaacaatag tgaatacata tttgtaaaag agtagaaatt ttattaaatg ttaaatgaca
    10201 ttgttaaatt taggtaaaat atacttagtg tgtattgaat tgaacttgat tttaaaatat
    10261 aattattctt aaatcaaaaa aagaaaggag tggtcttcca aaatggaaaa tcacaaacag
    10321 atgccttggc attcaagagc aactcatgaa attttagaaa gtctagattc atcagaggag
    10381 gggctcaccg ataccgaggc ggaaaaacgg cttaaagaga atggtttaaa tgaacttaga
    10441 aaaaaaccaa atagaacaac tttgcaaatg ctttggagtc aaattacaga tgctatggtt
    10501 ctgatactaa taggagcagc aatattgtcc ttgatatttg gagaatttac agaagctttt
    10561 gtaatactaa taattgtaac agtaaatgca gttattggaa ttgttcaaga gaaaaaggca
    10621 gaatcatcat tagaagcatt aaaaaatatg aactcaccaa cagctcgtgt tatgcgtgat
    10681 ggtgaagaaa atgttattcc agcaagtaat cttgtagtag gagatattgt ttttttagaa
    10741 gatggagcga tggttccagc agaccttcgt ttgatagaaa catctagttt aaaaattcaa
    10801 gaggcttctt taacaggaga atctgttcca tcagaaaaag atgcagatac aatcttacct
    10861 aaagaatatg tattaggaga ccgtagcaat atggcgtata catcttcaat tgtcacatat
    10921 ggtagaggtg taggtgttgt agtagcaact ggtatgaata cagaagtggg taacatagca
    10981 catcttttag attcacaaga tgattttgat actcctataa aacgtaaact taatactgtt
    11041 ggaaagacat taagtgtaat aggtattatt gtatgcgttg ttatatttgc aataggtgca
    11101 ttttatcaga gacctctctt gccaatgttt atgattgcaa tttcccttgc catatcaatt
    11161 attccagagg gattacctgc aacagctact attgtaatgg ctttaggcgt acaacgtatg
    11221 tctaaaagaa atgctttaat tagaaaatta cctgctgttg aaacattagg aggagctaca
    11281 gtaatatgtt gtgataaaac tggtacatta accttaaata aaatgactgt tacacatatt
    11341 gctgtcaatg gagattttga atctgggaaa gtaacatcaa tagatactgc atctgaaaaa
    11401 catccgttag tgtataaaga gattgtttat gctggtgcac tttgtaatga tgcatgcatt
    11461 aatccagata aaactgatga aatattaggt gacccaacag agggtgcatt aatttttatg
    11521 gccaaaaaat ttggaataga tcaggaaatt cttgaagaag aatatccacg tgtatttgaa
    11581 cagccttttg attctgatag aaaatgtatg acaactgttc ataatataga tgaaaagatt
    11641 attgcttata ctaaaggagc agtagacgaa attcttgacc tatgtactaa aatattgact
    11701 tcaaaaggtg aaagaaacat taccgaattg gataaaaaga atatacatga gttatgtctt
    11761 aacatgtcta aagatgcatt gcgtgtactt ggttttgcta agcgtgagat ttcatctata
    11821 ccgaaagaag atagtgaaaa catagaatat gatttgacat ttataggtat agttggtatg
    11881 attgacccac caagaactga ggtaattgat gcagtaagta cttgtaaaga agcgggtata
    11941 agaaccataa tgataacagg tgaccataaa gttactgcca caacaatagc tcatgagtta
    12001 ggaatatgga gtgaagaaaa tactgtgata tctggtgatg aacttgataa tttgagtgat
    12061 gatgaactag atgaagctgt taaaaatact acagtatttg ctcgtgtatc cccatttgat
    12121 aaacttagaa ttatacaatc tttaaaaaga attggagaag taccagcaat gactggggat
    12181 ggtgtaaacg attctcctgc cttaaagtct gctgatattg gtatagctat gggtataagt
    12241 ggtacagatg ttgcaaaaga ttcttctgac atgattttaa tggatgatag ctttacgaca
    12301 atttcttatg caataaaaga aggtagacgt gtatatagaa acatacaaaa agttatacag
    12361 ttcttgcttg ttggtaatat agcagagata cttacattgt ttgtagcaat attattaaat
    12421 tgggatactc cacttcttgc tgttcatatt ctgtgggtaa accttgcaac tgctactctt
    12481 ccagcccttg cgcttggagt tgatccacca agcagaaata taatgaagca tcagccagtg
    12541 aaatcaacca ctttatttga aaaaaatctt atacaaagag ttgttactca aggtatattt
    12601 gtggcaataa tgactatttt agcatataaa attggtctta atatagaaaa tcattctgta
    12661 ggtcaaacaa tggcattttg tgtattagca ttttcacaga tgttacgagc atttagtcag
    12721 cgttcaaata cagattcaat gtttaataga aacaatggta aaaatccatt tcttttaatt
    12781 tcatttttaa cttctgcatt gctaatagca gtgatactgt ttgtacctgt ttttagaaat
    12841 gcttttaact taacaatttt aaatagctta gaatggctta taacgattgc tttggctata
    12901 atgtctgtag ttcaagtaga ggttgggaaa attattaaaa gaaaaaaatt aaaaaatagt
    12961 tgacaaaatt aggaaaaata tataagatat aaaacataga aaaattaaat aaatcttatc
    13021 cagagaggtg gagggactgg ccctatgaaa cccggcaacc agtatattta tatacaatgg
    13081 tgctaaatcc tgcggcgaaa gctgagagat gagtataatt aagcttatac agagcctgag
    13141 ttatatactc aggctttatt tattttaact ctcatctgat aaatagttta attttttact
    13201 aaaatctaag ggggatacaa gaatgtataa taaagaaaca atatgtgtgc aaggaaatta
    13261 taaaccaggt aatggagaac caagagtatt acctttatat caaagtacaa cttttaaata
    13321 tagcagtata gaccaacttg ctgaattatt tgacttaaaa gttgatggac atatatattc
    13381 aagaataagc aatcctacta ttcaagcttt tgaagaaaaa ataagtttac tagagggagg
    13441 agtagcttct gtagctgtat catcaggtca atctgcaaat atgttagcag ttttaaatat
    13501 atgtaaatca ggagatagta tactttgttc ttcgaaagta tatggaggaa cattcaattt
    13561 attaggacct agtcttaaaa aatttggtat agatttaata tcatttgact tagattcaag
    13621 tgaagatgag atagtagaac ttgcaaagga aaatactaag gttgtgtttg cagaaacact
    13681 tgcaaatcca actcttgaag ttatagattt tgaaaaaata gcaaatgtag ctaaaagaat
    13741 taatgttcca tttattgttg ataattcatt agcatctcca gtgctttgta atcctttaaa
    13801 gtatggagca aatatagtta ctcattctac tacaaaatat ttagatgggc atgcttcaag
    13861 cgttggagga attatagtgg atggtggaaa ctttaactgg gataatggaa aatttccaga
    13921 attagttgaa ccagacccaa cgtatcatgg aataagctat actcaaaaat ttggaaatgc
    13981 tgcatatgca actaaagcaa gagttcagtt gcttagagac tatggaaatt gtttaagccc
    14041 attcaatgca tatcttacta atttaaatgt tgaaacacta catcttagaa tggagagaca
    14101 tagtgaaaat gcatttaaaa tagctaaatt tttagaaaaa catgaaaatg tagattggat
    14161 taattaccca ggacttgaag ataacaagta ttatgagaat gccaaaaagt atttatcaaa
    14221 aggatgtagc ggagtcttat catttggagt aagaggtgga ttagaaaatg ctaaaaaatt
    14281 tgtggaaaaa ctacagatag catctttggt tacacatgtt tcagatgtaa gaacttgtgt
    14341 tatacatcca gcttcaacta ctcatagaca attaacagaa gaacaattaa ttgcatctgg
    14401 agtattgcct tcgctaataa gattgtctgt tggaatagaa aatgtggaag atttaatagc
    14461 tgatttaaat caagctttaa atttttaata ttctaagaga aagagaagtg aaaagatatg
    14521 gctttaatac taccaaaggg attacctgtt attaataaat tattagatga aggaatagat
    14581 gtaatttaca aagaagattt taaaaagaaa ttaacatatg aagaaaacat agatactaag
    14641 atagccatac tgaatctaat gcctataaag atagatacgg aattagattt attaagaagg
    14701 atagataaaa caggattcaa tgtgtctgtg gaatttatta aaatttcaac aagagaaagt
    14761 aagagaagtt gtaatgaata tgtcaaagaa ttttataaaa catttgatga agccaaagga
    14821 gagtactttg atggttttat aataacagga gctccagttg agcaaatgga gtttgaagaa
    14881 gttgattatt ggaatgaatt agaagaaatt atggattatt ctaagagtaa aactaaatct
    14941 acattatata tatgttgggc agctcaagca agtctttaca aatactacaa tgtaaaaaaa
    15001 cttccattaa gtcaaaaatg ttttggagta tttaagcata aggtagataa aaatagtaaa
    15061 atagtagatg gatttgaaaa tgaatttttt gctccacatt caagacatac tactgtaaat
    15121 attgaagctc tgaaagagaa taaagagttg agcattgtga gtcattctaa agaagctggt
    15181 ccttatataa ttacaaattc aagagacgtg ttcgtaatgg gtcatagcga gtatgacaaa
    15241 tacacccttg acaaagagta taagagggat ataaacagag gagataaaat atcgatacct
    15301 caaaattact atatcaatga cgacccttca gaagagccaa ctgtaaaatg gaaaaaacat
    15361 tcggaactac tttttagaaa ttggataaaa aactatttga tacaataaat tttaatagta
    15421 aaatatcaac taaataaaat ataaaaataa taaggtataa agttaatttt aacgattctt
    15481 tataccttat tattttaaaa aaattgacct tataatagta gttttatata taagataata
    15541 aaattccaac tgaagttaca atattgtaat tgacaataga tacttaatat tataatattt
    15601 aatacattaa acattatagt attaagtatt tatttacaag atacacttaa gcctaaagtt
    15661 aatagttagt gaaataaagt atttttgtaa ggagcgtttg tatggatgag atgagtttta
    15721 agaaagagct actagagttg actagagata taaatatgaa atttacaact ttattaacta
    15781 aattttatca gccacttggt attacagcag ttcaagcttt gattttgtct gagttgtgtg
    15841 aacatggaga gaaaaaaata agcgatttag ggaaaaactt aaacatgaca aatagtaatg
    15901 tatcagtaat ctgtcaaagg cttgaaaaaa atggtttttt aaatcgtata agagatatag
    15961 aagaccaacg aattgtaaaa gtaaaagtta ccaataaatc attggatata caagagaata
    16021 tttcaagtag tatttttgat aattattttg aaaatatgac tagtgaaaaa ttgcaagaca
    16081 tggaagatat aatagaaggt ttagagaaat taaataaact tttaacctac attgattgca
    16141 aataacataa aataatttaa aggagaataa aattatggat gaacataaac cccaaaaaag
    16201 gtctgcactc tattattata cagttataat aatagtagta atgtttttaa atgctttaat
    16261 atttccaagt ttaatggata agaaaatggt tgagattgac tatgggaagt ttcttacaat
    16321 gttggacaca ggaaaggtta aatctgttga gatagaaaac aatagaatag caataaatcc
    16381 atctagtagt aaggataaga atatatatat aactggtaga atggatgacc cagaattggt
    16441 agatagacta aaaaatgcaa aagtagaatt tactaaggtt attcctaagg aaaattcacc
    16501 tcttttaaac attttactta catggataat acctataggt attcttatgc tatttggtaa
    16561 tttaatgatg aagtctatgc aaaagcgaat gggtggtaat gctatgcaat ttggaaaaag
    16621 caatgccaaa atgtatgtat ctgctcaaag tggaaaaaga tttaatgatg tagctggtca
    16681 agatgaggca aaagaagctt taagtgaaat agtagatttc ttacacaatc cagaaaagta
    16741 taaaaaaata ggagcacaaa tgcctaaagg tgcactttta gtaggacctc caggtacagg
    16801 aaaaacacta cttgcaaaag ccgtagcagg tgaagcaaat gtaccatttt tctcaatatc
    16861 tggttctgag tttgttgaga tgtttgtagg aatgggagct tctaggattc gtgatttatt
    16921 taaacaagca aaagaaaaag ctccatgtat agtgtttata gatgagattg atactattgg
    16981 taaaaagcgt gataatggaa gtggactagg tggaaatgat gaacgtgaac aaactttaaa
    17041 tcagttgcta agtgagatgg atggttttga tggtggaatt ggagtagtaa tacttgctgc
    17101 tacaaataga cctgacagtc ttgacaaagc attacttcgt ccaggaagat ttgatagacg
    17161 tataccagtt gaacttccag accttggagg acgtgaagct atattaaagg tacatgctca
    17221 aaatgtaaat gtaagttcag atatagatta taatgcaatt gcaaggtcta catcaggagc
    17281 atcaggtgca gaacttgcaa atattgtaaa tgaagcagca cttttagcag ttaagtctgg
    17341 tagagatata gtagagcaac aggattttga tgaatctgtt gagactgtaa ttgctgggta
    17401 tcaacgtaaa ggagctgtaa tttcagacaa ggaaaagaaa attatagcgt atcatgaaat
    17461 aggtcatgca cttgttgctg ctatgcaaaa acattctgca cctgttcata agattactat
    17521 aatacctaga acttcaggag cattgggata tactatgcaa gttgctgaag atgagagcgt
    17581 ccttatgtct aaagaagaag caattgacaa aataactact tttacaggtg gtcgtgctgc
    17641 ggaagaggtt atttttaaca cttgtacatc aggagcatca aatgatatag aacaagccac
    17701 taaaattgca agagctatgg tcactcgtct tggtatgagt gaagaatttg atatggttgc
    17761 tcttgaaact gtaagcaatc aatatttagg aggagatact tcccttgctt gttcttccga
    17821 cactgctgct aaaatagatg aagaagtatt taaaatagta aaaacttgtc ataaaaatgc
    17881 aattgaaatc ttagaaaata atattgataa gttgcatgaa cttgctaagt atttattaga
    17941 aaaagaaact ataacaggtg atgaatttat ggaaatactt aacttaaact aaaatgatta
    18001 tacacaaagt aaaaatgcct attgttaggc atttttattt tgtaattaag ttttttaaat
    18061 tcatatttaa atctttacat aatcttaatc actaaaaata catcttaact acaattttat
    18121 attggaattg atattataat agtaatgaat gatgtaagga ggttttttaa tggatagacc
    18181 gaatcctgat attctattag aaaaaataaa aaatgaagaa gaaaaactaa gtagaggtca
    18241 attaaaaata ttctttggtt atgcatcagg agtaggaaaa acctattcta tgttggaatc
    18301 tgcacaaaat ttaaagaaag taggtgttga tgtagtagtt ggatatattg aaccacacac
    18361 tagaccagaa accttggctt tacttgatgg attggaaatt ctccctgtaa aggagataga
    18421 atataaatct ataaaattaa aagaatttga cttagatgct gctttagcaa gaaaaccaga
    18481 agttatatta gtagatgaat ttgcccattc taatgttgct gggttaaggc atacaaaaag
    18541 atggcaagat atagaggaat tgctgttagc aggcataaat gtctacacta ctgtaaatgt
    18601 acaacatcta gaaagtctaa atgacattgt tgagataatt acaaacgtat cagttaaaga
    18661 gactatacca gataagtttt tggatacaaa tacacagtta gaacttatag atgttgaacc
    18721 tgatgtactt cttgaaaggt tcaatgaagg taagatatat aagaaagaac aagctactag
    18781 agcaaaaaat aattttttta taaaagataa cctagttgca cttagggaaa ttgcacttag
    18841 aaaaacagca gaaagagtca ataaagaagt tcaaatgacc agactatcaa agggtgatgt
    18901 aactgtaata cctacatcag atacgcttct tgcatgtata agtccatctc catcttcagc
    18961 aaaagttatt agaactgcat ccagaatatc agacagtaca tttgcaaaat ggatagcttt
    19021 atatgttgag actccaaaca cagcaagact tccatttgac gaacaaaaac aactacaaaa
    19081 taatctaaaa ctagctaaga agctaggtgg agaaataatt gttttacatg gtgaaaatat
    19141 aatagagcaa atacttagaa ttgcaaaact gagaaatgta acaaaaattg taataggtag
    19201 aaatcattca agtaataaaa ggtttagtaa aaagtttaaa aaagatatag ttgacaaatt
    19261 aattgatgaa gttgattata tagatataca tataattcca tataaatttg cctcagatgt
    19321 taaatacaaa cctaaaaagg ataaagtgtc aataaaatct aaatttaaaa ttagtaaagt
    19381 tgattttata aaacttatat ttattacttt gatgataagt attttagcat atgcagtaca
    19441 aagtatgggc tttataagag aaaatatatt gttaatatat atgttgggtg tggttttagt
    19501 ttctatgtgg actaaaggtt atagtacagg tataataagt tctgtattta atatagttct
    19561 gttaaattat ttttttacag caccattata tacattatct atagcagatt caaactatat
    19621 agtaacacta gttgtatttt caatagtagg tataataact agtactctta catctaagat
    19681 acaacatgaa gcagaaactg cttctaaaag agaagaaaat acaaaaatga tatatcaaat
    19741 aattaaggga tttttaagat tatcaaataa agacgatata ataaacaaag gtatagaact
    19801 tttgtctctt agcttaagta gagatgtgat ttgttattta aatgtagata agtataacaa
    19861 atcgaagctt tataaaaaaa atactaataa taaggataaa aatgatttgg atagtgaaga
    19921 tgaaaaagct gttgcaagtt gggttttatc caattcaaca gtagcaggaa atgatacgga
    19981 tacacttcct ggttcaaagg gatactatat accaattgta gggatgaatt taactttagg
    20041 agtagtagga atatcatgtg ttgattcaaa attggataca gaagatataa gcttaattga
    20101 aacaataatt gctcaaatgg caatagcact tgatagagaa atcttgtcag aagctaaaga
    20161 aaataccaat ctcgagattg aaagggaaag gctaagaagt aatcttttaa gagcagtttc
    20221 gcatgacctt agaactccac tagcaggaat atctggcgca gtaagtacaa ttataaaaaa
    20281 caagggtact ataggtcaag atataataga tgaattgctt agtggtgtat atgaagatac
    20341 tcaatggtta ataagacttg ttgaaaattt acttagtatg accaagatag atgaaggaaa
    20401 attggaagtt aaaaaacatt ctgaactagt tgaggaaatt atatcagaag cattacaaaa
    20461 aataaaaaaa agaattgaaa atgcacttat agatatcaat ataccagaac agatattatt
    20521 tgttcccatg gatgcaaaat taatagaaca agttttaatt aatttaatag acaactcttt
    20581 gaaatattca aaggaagatt gtaagataga gataaatgta tatgagaaag atgattatgt
    20641 ttggtttgaa gtatctgata atggtcctgg tatatcaaag gaacttaaaa agcatatctt
    20701 tgacagattt tttactggag aagaaggagc taaagattcc agaaagggtg ttggacttgg
    20761 attatcaatt tgtaagtcta taattcaagc tcataaaggt gaaattatgg tagaagacaa
    20821 taaagataag ggctcaactt ttaagtttag tttgcctaag gaaaatgaat agttttaaat
    20881 tttaaaatta gttttggagg aagaaatgag taaaaaactt atattgttag tagaggatga
    20941 taagacaata agaaaattta tatctactgc acttttaaca caggattatg atgtcaagga
    21001 ggctataact ggaaaagaag gtatatctat tgctgtttca tattctccag atgttgttct
    21061 cttagattta ggcttagaag atatggatgg aatagaggta ataaaagcta tcagacaatt
    21121 ttcaaatata ccaataattg tagtttcagc tagagagcaa gatagagata aggtagaagt
    21181 atttgatgca ggggcagatg attacttaac aaaaccattc tctatagtag agttattagc
    21241 aagagtaaga gtcgcattta gacattcaca agtagaagtt caacaaaaag atgatgttaa
    21301 atctacgttt gaagtagata aattattgat tgattttgat aaaagaaaag ttattgttga
    21361 tgatattgaa gtccatttga cacctattga atataatatt ctttcattac ttgcaaagca
    21421 tcatggaaaa gtgcttactc ataactttat aataaaagag atatggggta gtgttatagg
    21481 gaatgagact aagtctttaa gggtctttat ggcaacatta agacgaaaaa tagaaaaaca
    21541 acctgcaaac ccaagatata taatcacaga agttggtgta ggatacagat taaatgatga
    21601 atagttgtaa tatatattaa ttattagact atgtttttat taatatatta aaattatata
    21661 gtttagttaa aataagaatg aagtgtatgt gctgttgtca gtttagcaca tgcattttat
    21721 tttttaaatt tttttagtat caataatttt ttagtattaa taatatgaaa tatgttt
//
LOCUS       DAMJVM010000057        21693 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.059, whole genome shotgun sequence.
ACCESSION   DAMJVM010000057 DAMJVM010000000
VERSION     DAMJVM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21693
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.059"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            277..2511
                     /locus_tag="PC523_002312"
     CDS             277..2511
                     /locus_tag="PC523_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2475828.1"
                     /translation="MQNHDYIKMLNKKVKLLSKELDNRNFKLKSSNEIDFFSKMPNRE
                     FMYQYIDDCISKNVHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQKLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELSTMGTTIVESARELKIQLPNEKEIYSNITFSIGGAI
                     WNGESNMTTLMLFNQVYSALKKAKSEGKNRYTLFDFKDNTFYNKLENDKEFLSEISYA
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     YLFEENCRNIRKWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLTELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKQFEKLLSKGNNSEKMFIKYSDIGKALIEKIFDGFFIMQDMKKLEANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEAAEHQ
                     KYSNFYFSANCIISENKLLLSKLHLDLINTAGYINEFESYSIPKMANTHLDESDILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMLDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLEMIDTNKIEPFLYRFIKRDGQIATVMYTQCNLSAIDGRSIAQGMYVDINKIKN
                     LTKI"
     gene            2542..3387
                     /locus_tag="PC523_002313"
     CDS             2542..3387
                     /locus_tag="PC523_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2475829.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRITFINDNLKNLIKSYMQDNPLG
                     MTNVEIAKKLPDNIAKFFTDYHNIQLNWEKDYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKSYIITMANDITKLYEENKKNLHYSITDPLTGAYNRKYLNDRFDMFIGDYIVL
                     IDLDNFKMVNDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQIEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKKKKKF
                     NKNNY"
     gene            3627..5360
                     /locus_tag="PC523_002314"
     CDS             3627..5360
                     /locus_tag="PC523_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2475830.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDIDEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQYEIPSCDINNDIFEIVSEYENRCMVLGKYTVRTKENAQMIL
                     EVNQRCTFEIIEDREKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYNEFMEMSGGSAVGAVYPPD
                     LPKALEDCEQCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDMENDNFIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLRGNLHETIEIREINSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIQEYLCSSDSKNND
                     ALLIIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTYDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSVYE"
     gene            5827..6171
                     /locus_tag="PC523_002315"
     CDS             5827..6171
                     /locus_tag="PC523_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2475831.1"
                     /translation="MKNELYLICLNKRFNLWVQPTFVAPFKELLIDNNLRDIKLHKLR
                     HANATLILLSSTNIKSISEILGHADIKITMNRCSHLLYEMDNEASENTIQIMFSFTSI
                     KCQLKHFFLLIS"
     gene            6362..7717
                     /locus_tag="PC523_002316"
     CDS             6362..7717
                     /locus_tag="PC523_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2475832.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISAITNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIIFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATVIASISVFAVCI
                     AFSEPIVSLFIHPEETILFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(7835..8503)
                     /locus_tag="PC523_002317"
     CDS             complement(7835..8503)
                     /locus_tag="PC523_002317"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HDF2475833.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKDAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLVKDGYGY
                     LEIK"
     gene            complement(8570..8998)
                     /locus_tag="PC523_002318"
     CDS             complement(8570..8998)
                     /locus_tag="PC523_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2475834.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGIFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            9110..9703
                     /locus_tag="PC523_002319"
     CDS             9110..9703
                     /locus_tag="PC523_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDF2475835.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENATKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWIL
                     IIACILLFVFPQKIQSIFSLLLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            9823..10758
                     /locus_tag="PC523_002320"
     CDS             9823..10758
                     /locus_tag="PC523_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDF2475836.1"
                     /translation="MNKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SIGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVVGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMDVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKSRNSYQGCKFV"
     gene            complement(11060..12004)
                     /locus_tag="PC523_002321"
     CDS             complement(11060..12004)
                     /locus_tag="PC523_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2475837.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLASEQVAFTRLF
                     FGFIVLGIYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIVGVSIAAVL
                     LYTSPLFLAIFSKVCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGI
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSKPWEILNYANNLDVLFC
                     MLMLGIVPAALAYIFYATGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSSKLKQIEKTESI"
     gene            12322..13764
                     /locus_tag="PC523_002322"
     CDS             12322..13764
                     /locus_tag="PC523_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2475838.1"
                     /translation="MNLNLELDLEFYKKILEASHDEICVSDDKGIIIYCNKAFEENYG
                     LKKEDILGKNVSFLEDSGYSTKSPIPVVLKTKSKFSLEQDTQTGKKLIITATPIFDEN
                     GNLEFTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVM
                     RPIINTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRANGPFITINCATISPQLL
                     ESELFGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFT
                     PVGSLKNKNVDIRIISATNADLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVE
                     IIKYYFNRYSSDFNLNKTISKEAMETIANYRFPGNIRELQNIIQKILLTCTDNHITIH
                     NLPNILTKNINITNNGNKTHISQINKVITPDSKSINYKNKNFDTLIKEYEKNIILDAY
                     EKFGSSYKVAKHLEISQSKANRLIRKYTNT"
     gene            13964..14548
                     /locus_tag="PC523_002323"
     CDS             13964..14548
                     /locus_tag="PC523_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HDF2475839.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(14642..15421)
                     /locus_tag="PC523_002324"
     CDS             complement(14642..15421)
                     /locus_tag="PC523_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2475840.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDMVAIEEIEKKGVQFNVATGRI
                     FKQARNIIEESLDMNGYYICENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(15479..16735)
                     /locus_tag="PC523_002325"
     CDS             complement(15479..16735)
                     /locus_tag="PC523_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDF2475841.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFAESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILRKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEGEAFKLIKPVEEKIKSRVGKYVYTEGDISVSEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVAYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVRKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            16993..17892
                     /locus_tag="PC523_002326"
     CDS             16993..17892
                     /locus_tag="PC523_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDF2475842.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVGEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            17916..19364
                     /gene="ddlR"
                     /locus_tag="PC523_002327"
     CDS             17916..19364
                     /gene="ddlR"
                     /locus_tag="PC523_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HDF2475843.1"
                     /translation="MIFSNLKLNDNEPIYIQIKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSDGLIYSISGKGTFVKSKNKSSNTTWSLNWDYLENTYSKK
                     ASELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLKYMTNKGVDISNKDILITNGFTEGFDIVMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFDMLEYNLIKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYECVKKHIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            19423..20229
                     /locus_tag="PC523_002328"
     CDS             19423..20229
                     /locus_tag="PC523_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2475844.1"
                     /translation="MNLILGDNMSKKSKRKKINKLKLTIVITLLFLALLSIYEILFSN
                     PNKKDNVKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     INLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVISNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIRKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            20255..21439
                     /locus_tag="PC523_002329"
                     /pseudo
     CDS             20255..21439
                     /locus_tag="PC523_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861202.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
BASE COUNT         8266 a         2892 c         2940 g         7595 t
ORIGIN      
        1 catctctcaa tctatctatt tatgaaaaaa tatgatataa ttgcattcaa aaaggaaagc
       61 tttcaatata ctagaattta agcattaact aattttatct aatattatta ctaggttaaa
      121 aaacatttgt aacatttgaa caaataactt aatgtatatt aattttaaat acgtatttaa
      181 aattaattgt ttaaattatt tatagattaa aatttaaata cttctagcat aaatacagtt
      241 gtcgtcaaaa tttaaagtat cttatggagg aataccatgc aaaatcatga ttatataaaa
      301 atgttaaata aaaaggtaaa actattatct aaggaattag ataatagaaa ttttaaatta
      361 aaatcctcaa atgaaataga ttttttttct aaaatgccaa atagagaatt tatgtatcaa
      421 tatattgatg actgtatttc gaaaaatgta catataggaa tcatgttgtt aaatattgat
      481 aattttaaat tatttaatga tatatatgga tatctagtgg gagaaaattt attaattact
      541 ttaactcaaa aactattaca ctttattcca gaatcaaaag ggatagttgc ttatttaaaa
      601 ggagaagggt ttttaattgt acttccaaat gtatcaaata aagaattaag tactatgggt
      661 acaactattg tagagagtgc tagagaattg aaaatacagc ttcctaatga aaaagaaatt
      721 tactctaata ttacatttag cattggtggt gctatctgga atggtgaatc aaacatgact
      781 actttaatgc ttttcaatca agtatatagt gctttaaaaa aggctaaaag tgaaggtaaa
      841 aatagatata ctctatttga tttcaaggat aatacctttt acaataaatt ggagaatgat
      901 aaagaatttt tatccgaaat atcatatgct ttacaaacta aacaaattga agtattttac
      961 caaccacttt ataatataaa aaataatact atagttggat gtgaggctct cgcacgatgg
     1021 agacacccta ttaaaggact tctcactcca gataagttta ttcctttatt tgaacagttt
     1081 ggtttgataa cagaactaga tttatattta tttgaagaga actgtagaaa tataagaaaa
     1141 tggattgata acaatgatgt ttttgttcca atttgttgta acttttctcg tcttcatttt
     1201 tttaatccta attttccaag tatgctaaaa ggtattactg aaaagtacag cgtctctaca
     1261 tcaaatttca gcattgaaat aacagaaaat atattaatag aaaatacaaa tacaattatt
     1321 caacaactaa ctgaacttcg ttcctatggt ttttctgtat caatggacga ttttggttct
     1381 ggatattcat cttttggtat gttgcaaaat ctgccttttg atataatcaa aattgataga
     1441 atatttttat ctcataattt aaatgatttt aaaaatacaa ccattttgta ttctataata
     1501 aacattgcaa gagctcttgg gatgcttaca gtatgtgaag gtgttgaaac agaacaacat
     1561 gtagaattta ttaaaagtat aaattgtgat atagctcaag gttattatta tgcaaaacct
     1621 atggagtgca aacaatttga aaagttacta tctaaaggta ataattcaga gaaaatgttt
     1681 ataaaatatt ctgatatagg aaaagcttta atagagaaga tatttgatgg attttttatt
     1741 atgcaagata tgaaaaaact agaagcaaat gttactgaag acattcaatg gtttgatttt
     1801 tacaacaaaa ctgaaattac atcttttcct aaagtaatag aacattttga aaaacacatt
     1861 aaaggtaaaa aattttctat cctttacaaa agtataaccc cacataatac tgaatcagat
     1921 tcattattga ttagtggaga agctgtttta tcagacgaag ctgctgaaca tcaaaaatat
     1981 agtaacttct actttagtgc aaactgtata atttcagaaa ataaattact cttatcaaaa
     2041 ttacatctgg atttaattaa tactgctggc tatataaatg aatttgaatc atattcaata
     2101 cctaaaatgg caaatacaca tcttgatgaa agtgatattt tatcacaatt ttattcaatg
     2161 ttgcctgtag gtataattcg atacgattta tttggtgata tgattattac ttatatgaat
     2221 gaagaaatgc ttgatattat aggttataca aaagaacagt tttttggtga gatgggcgga
     2281 aatttaagag caattgttca tccagatgat ttagactttg tttataaaaa aagtttggaa
     2341 atgattgaca caaataaaat tgaaccattc ttatatagat tcataaaacg agatggtcaa
     2401 attgccacag ttatgtatac acaatgtaac ctttctgcaa ttgatggaag gtcgattgct
     2461 caaggtatgt atgtagatat aaataaaata aaaaatttaa caaaaatcta gtacaaagta
     2521 ttttattttg gagaaattaa tatgtttaaa gaaatttttt taagaacttt tccaggattt
     2581 ctaattataa gagattctaa ctatagaatt acatttataa atgataattt aaaaaattta
     2641 attaaaagtt atatgcaaga taatccactt ggaatgacta atgtagaaat agctaaaaaa
     2701 ctccctgata atattgccaa attttttacg gattatcaca atattcaatt aaattgggaa
     2761 aaagattatc cttatgataa aatatcaaat tggattttag aatttaaaaa agatactaca
     2821 agttattgga atgttttaga atacaaagta gatgttgatg aaaaatccta cataattact
     2881 atggccaatg atataacaaa actgtatgaa gaaaataaga aaaatcttca ctactcaatt
     2941 actgacccat taactggagc atataataga aaatatttaa atgatagatt tgatatgttt
     3001 ataggagatt atattgtact aattgactta gataatttta aaatggtaaa tgattatgaa
     3061 ggtcataatg taggagacaa gattttatgt gattttgttt cacttttaaa taaagaattg
     3121 ataaattcta cttctattat aagattagga ggagatgaat ttattgttat attctctagt
     3181 gatgttgaca aaaattatgt ttattcacag atagaagcct taagagaaaa ttttttaaaa
     3241 gtattctcta agtataagta tctttcattt agttatggtg ttgatacagt aaaaagaaat
     3301 ttaaaattga caatagttga attggataaa aaaatgtata aaaacaagga aaaaaagaag
     3361 aaaaaattta ataaaaataa ttattagcat tgtcatttta aaataaatta aaattagctt
     3421 tcttttttaa aacttttacc ttagcttata aatatgtatc tatacaaggt attaatatta
     3481 aatactgaaa catatattgt ttcagttctt ttttgttaaa attaacttat tcaataaaga
     3541 tatcatagtt caacaaagct tttaatttaa atatattgat gtataatata attaaaatat
     3601 aatagcaaaa agaaaggagg aatatcatga gcatactcct aaaaaaagca cccaaattag
     3661 caaaacatat tataacaagt ttttatataa atcgagatat tgatgaagta ttaaaatacc
     3721 tttgtgaaaa tgttacatgg attggaccag gagaacagga atttctcact tcttttaatg
     3781 aaataaaaaa ttatttttat gcaggtcaat atgaaattcc ttcttgcgat ataaataatg
     3841 atatatttga aattgtaagc gaatatgaaa atagatgtat ggtattagga aaatatactg
     3901 ttcgaactaa agaaaatgct caaatgattt tagaggtaaa tcaacgttgt acatttgaga
     3961 taattgagga tagagaaaaa ttattagtaa aacacatgca catatcaaac ccttatggtg
     4021 aaatgcaact tgatgaatac tttcccacta aaattggaac tcaaagttat gattatttgc
     4081 aaagattact aaaggaaaaa acagaagtta ttgaaatgat tacaaataat attaatggtg
     4141 gcttaaaagg tagcaatgat gatagtacat actcattttt ctatgtcaat gaaggattgc
     4201 ccaagatatt aggatacacg tataacgagt tcatggaaat gagtggtggt tcagcggtag
     4261 gtgctgtata tccaccagac ttacctaaag cactagaaga ttgcgaacaa tgttttgcaa
     4321 aaggacctac atattcatct gaatatagaa tacgaaaaaa agatggaaca ttaatgtggg
     4381 tattagattc tggaatgaaa tcattaaata gtgatggcat agtaaaaatt aatagtatta
     4441 ttactgacat aacccaacta aaaaacatag aatctgaact aaaattagaa agagaacgat
     4501 atcgtattgc tttgcaaaat attacagata tcatgtttga gtatgacatg gaaaacgata
     4561 actttataaa atatcaacgt gtagaaattg acaaaaaaat tgaattggaa aattttgaaa
     4621 caaaaaatta ctctaagcta ttagaatctg gtaaaattat tcaccttgat gatattggaa
     4681 aactactaga agtacttcgt ggaaatcttc atgaaacaat tgagataaga gaaataaatt
     4741 cattaactaa aaatgaatgg agatggattc gtgttcaatg ttcagttata tatgatagtg
     4801 accataaccc catcaaaaca ataggcgttt taaaagacat aactgaagat aaatctaaat
     4861 tagaaagctt aattaatcag gctcaacgcg acccactaac acaattgtat aatcaaagag
     4921 tatcacaaaa tcttattcaa gagtatctgt gtagttctga ttccaaaaat aatgatgctc
     4981 tattaattat tgatattgat gattttaaaa ctgttaatga tacttttggt catttagagg
     5041 gaaatgaagt tctagttgct gtatcaaaaa tactattaca taacacatat gataaagaca
     5101 tagttgctcg catcggtgga gatgagttta caatatttat aaaatcatta acaaaggatt
     5161 taataataaa aattactaat gatattttaa atgatgcaag taaaataaaa gtaaaggaca
     5221 atcataaaat aacattaagc attggtattg cttttactga tgattcaact aaattatata
     5281 aagatttatt ttctaaagct gataaagctc tttatttatc aaaagcagat gggaaaaatt
     5341 gttatagtgt gtacgaataa agccatttat atggctaaaa atgaaccttt aaattcaaat
     5401 tcatagtcta acaatcaaag agtcaaagct aatcttatag atagccaact agttactaga
     5461 aaaaatttag gcactatatt tattgatact gctcattttt ccaaagagca aatttatata
     5521 aaaaattaaa cttttatgta tgcacaaata ctaatgataa acattttgtt acaaacattt
     5581 atcattagta tgttctatct taaaacaaaa tgataattta tctatataaa aaagtgcttt
     5641 gttgacagag ttcacttttt tatatagata gtttaaatat gaatacatgt tttatcccat
     5701 acattttggt taatataaaa ccttgtatct tattatattc attcatctgt tcttaaaatt
     5761 tacataccac atagctaata taaaactaaa atcacccctt taattaactc actttctaca
     5821 tgtatattga aaaatgaatt atatttaata tgcttaaata aaagattcaa cctatgggtt
     5881 caaccaactt ttgttgctcc ttttaaggaa ctattaatag ataacaatct tagggatata
     5941 aaattacata aattaagaca tgcaaatgca actttaatat tactttcaag tactaacata
     6001 aaatctattt ctgaaatatt aggacatgca gatataaaaa taactatgaa tagatgttct
     6061 catttattat atgaaatgga taatgaagca tctgaaaata ctattcaaat aatgttcagt
     6121 tttacatcaa ttaaatgtca gttaaagcac ttttttctac ttatcagtta aatgtcagta
     6181 atatatatag taagatatac ttataaaacg tttgaaatca ctattcctta ataaatgtta
     6241 taattattta ctatttgctg ttgacattgg tattatacca tagtttatca tttatattag
     6301 aaattaaatt attaattaat agtttcgtaa ttttacaaca gatttacagg gagtgataat
     6361 aatggataca aaaatatcta taaacgaaaa catgagtcta ggtaaaagat tttttaaata
     6421 cctagcacca tctgtagttg caatgtgggt tttctcacta tatacaatgg ttgatggaat
     6481 atttgtaagt aaaggagttg gagaattagc tttagctgct gtcaatatat caatgccttt
     6541 tattaatttt atatttgcag tttctctatt attttcaact ggagcatcta ctataattgc
     6601 aatttatctt gggaaaaaag atattaagtc tgctaacgaa gtcttttcat ttaacttagt
     6661 atcaataatt atattatcaa taattatatt agctataact ttttttaatt tagacagatt
     6721 agcactattt ttaggtgcta cagaatctac aattggtatg gttaaagatt atttaggaat
     6781 aataatattt tttaacggat tctttatagt gtcttattct ttagaagtta taataaaaac
     6841 tgatggcttt ccaatattag ctactgttgg tgtaataata tctgctatta caaacatcat
     6901 acttgattac ttgtttgtaa ttgaatttgg atggggtgta aaaggtgctg gtattgcaac
     6961 tggtttgtct caagtctttt ctactatttt cttcttaata cattttttaa gaaaaaactc
     7021 tactttgaat ttttcaaaat ttagaattga ttttaagaca cttagaaaaa ttatatttat
     7081 aggttttcct gattcaacta ctgaattaag ctgtggtata gttgtcttac tttttaactt
     7141 aagtcttact aaatatattg gagaaaatgc tcttatttat tatagtgtca taaattatat
     7201 aaatacatta gtacttatga ctatgatggg tataactcaa ggtatgcaac cacttactag
     7261 tttttattat ggtgcaggaa atatagataa tgtaaaaaaa ctattaaaaa tggggataaa
     7321 agccacagta atagcatcaa tttctgtatt tgcagtttgt attgctttct cagaaccaat
     7381 agtctcttta tttatacacc cagaagaaac aattctattc aatgaaggag ttcgtgtctt
     7441 taaaatattc tctatatctt tcttattggt aggaattaat gtaataattt ctggattctt
     7501 tgtatcagta gaaaaaccat caatttctac tgttatatct cttggtagag ggcttgtaat
     7561 agttgtttta agtctaattt caatgatact aatatttggt ggtcaaggaa tatggatgac
     7621 tactattgtt tctgagttta tatgtcttat tttatcatta gtttttttaa aaaagaattt
     7681 ttctacatta gactctaatc taaataaggt tgcatagatg ataagagtgg caatatatga
     7741 aaatatataa ataaaaaata cctattatac aatcttgcaa acattaaacc tttgcaaata
     7801 ttaggattat ataataggta ttttatttat gtaattattt tatttctaaa tatccataac
     7861 catcttttac taaagttcct tttacttcat attctacagg ttttttaaat attggaccgt
     7921 ctacgacaaa tgtatgatac tctccatgtt ctccacatgg gtctgcacca gcatctttta
     7981 tcttctccac aagttctcta gttaattttt ttcctaaaaa ttcttctcct agatgcttta
     8041 agtttacttt tgttacaaca ctacaaaatc cactgtctat aaattcatat actaaatcct
     8101 ctctactctc ttgccataat ggtaaatcta ctttcatacc aactgcttca catctatcag
     8161 tatcccattt tctatggctt tctatatcta tatctccata tgcgcatatt gttgctccca
     8221 tgtttttagc ttttattaaa gccctctcaa aactctcttc atatccttct actccacact
     8281 cagcaactaa taaaggtaat cctatattgc tacttacttg tttaagcata ttatattcta
     8341 aattatgtgt ccatgatttt ccatgttctt cacttatagt tgtcaagagt gccacaggct
     8401 tatatccttt tttcaacatt ctatttaatg ctaatatact atctttacca ccactaaatg
     8461 acattacaaa tttttcatca gccaatttta acacctcttt catctaactt tgttttatta
     8521 acataattat accaaatttt tgagttgcat agcaaattat attaaattgt taattcacta
     8581 taaaaaatgt tcctaatatc aaaagtataa cccctatgta tttgtataaa tgcattgttt
     8641 cttgtcccaa aattacgcta tcaataaaaa atccaactaa aacttgggac acaagtgtta
     8701 atataagtgt atttgatact cctaaatttg gaattgcctt tacagtaaaa tatattatta
     8761 gacctccaaa tattcctcct aataacaact tataattaaa accatttata gatactaggc
     8821 actttaggtc acctgtaact attatacttg ccaaaaaaaa tattgctcct acagttaaac
     8881 ttataaatgt ggccactaat gctgttgtac tttttcctag ctctccattt ataaaagctt
     8941 caagagctgt cgaaccacct gctaataatg caaatattat tgcccataat tttgtcatgg
     9001 acaaacctcc ttctatataa ttctattata ttaatagaag tttatgatac aacctattaa
     9061 ttaattacta agtatagttt aatttttata cactaggctt tatttggata tgaatttctg
     9121 ttataattgt tctatcgatt ttggaggtga ttttatgtac acaaatttta atatcgattt
     9181 taataatttt aataaaaaag aaaatgctac taaatttatg ttaatgggag tattactcat
     9241 tatattagga ttattatgcc ttacatttaa gacattaggt ataaaattaa tttcttggac
     9301 ttttggtatt gcacttttat tctttgcata tttaaattta aaaaatatta atgaactaaa
     9361 aagatatgct actaaagaag aaattaaacc ctctattaat attcaatgga ttctaataat
     9421 tgcatgtata ttactttttg tatttcctca aaaaatacaa agcatatttt cattattatt
     9481 aggattttat ttaatcttta atcaactagt agcacttgta aatagcaaga ataatcctta
     9541 ttctaaattt acaacttgga atatagttaa gattctattt ggtatatgcc ttatattatc
     9601 tccacttttt ttatctagat ttatagtgtc gataatgtct tttttcataa tattatttgg
     9661 actagttcta ttcttctctg gaaatactgc gagaaaatat taattatttt ctcgcagtat
     9721 ttttatattg cttaaaatag aatttgttta agttatttat ttatggtaat atgttttttg
     9781 tacaatctca ttgtacaatt ttattagaaa taggtgattt ttttgaataa atttaaatat
     9841 gcttttgata ataaaaggta tcatacttgg aattactatc ttagaaatac ttttggagaa
     9901 aaagttttta aggtatctat aaatgcagga tttacttgcc caaacattga tggaagtatt
     9961 ggttatggtg gatgcacata ttgtagcaaa gaaggttcgg gtgattttgc tggaaatcct
    10021 aaagataatc ttatcagtca gttttataat ataaaagaaa tgatgttaaa aaaatggcct
    10081 catgcaaaat atattggcta ctttcaagcc tatacaaata cgtatgctcc tcttgaagtt
    10141 ttaaaagaaa aatatgagac tatattagaa cttgaagatg tagtaggtct ttcaatatct
    10201 acaagacctg actgtttacc agatgatgtt gtagaatact tatctgaact aaataaaaga
    10261 acaaacttat gggttgaact tggacttcaa acaattcatg attccacatc taaaattata
    10321 aatagaggtc atgattataa aacatttgtt gaaggtgttg aaaaattaaa gtctaaaaat
    10381 ataaaggttg ttgtacacat tataaatggt ctccctggtg aagattataa tatgatgatg
    10441 gaaacagcaa aggcagttgg taaaatggat gtagacggca taaaaatcca tctattacat
    10501 gttataaaag atactcctat ggaaaagatg ttacaaaatg gtatgttaac tcttatggaa
    10561 caagatgaat atatcaatct tgtctgcgac caattagaaa tacttcctga aactatgata
    10621 atccatagac tcactggtga tggtaaaagg gatgaattag taggtcctat atggagccta
    10681 aaaaaatggg aaattttaaa tcaaattgat gacaccttaa aatctagaaa ttcttatcag
    10741 ggttgtaagt ttgtttaata ctaaataatt attacaaata aaacaatatc tacaaaataa
    10801 taactataga aataattata taaatataat tatttctata gttatttagc ctttgcaaag
    10861 caaaatatta atttgtatat ttacacaata ataataaaaa atagataatc taaactgtag
    10921 atacttatat tttatttgta ttgtccactt tattctataa actttataaa cagttatttt
    10981 tttatactaa atataatttg gtataatttt tgttagactc tacagtgtat accaataaat
    11041 aatttttaag tatctagttc taaatacttt cagttttttc aatctgcttt aacttgctag
    11101 acttataaaa tattcttatt tcatcataag aaagattaag tgctactact gcagatatca
    11161 acatcaacat cactccaaat aactttccta aggaaaaact ttctcctaat atagtgcaac
    11221 caattattac tgaaccaacc aactctactg atgccacaac acctgcaacg gataactcta
    11281 ctcctttaga gatacctgtt gcgtaaaaaa tatatgcaag tgcagctgga acaataccta
    11341 acattaacat acaaaataaa acatctaaat tattagcata attcaatatt tcccaaggtt
    11401 ttgaagaagg aatcataaat atagctccaa ataagaaact gtatactaat attgttgaac
    11461 ttgaaaattc ctttaaagca tttttactaa tagtcggcat aagtgcatat gctattgctg
    11521 ataatatccc aagtaaaagt ccaaaagcgt ttaaaccttg aaaatccaaa cgtccaccag
    11581 tcactgccat aatggcgcca ataaaacaaa gaataagtga aaataattta cttctagtaa
    11641 tattttcttt ataacatact tttgagaata ttgctaaaaa caatggtgat gtatatagta
    11701 aaactgctgc aattgataca cctacaatgt caattgcttt aaaataacat aaattaaaca
    11761 ttgcctgaca tataatacct attattactg aatatataat tccttttttg cttattttta
    11821 agatttgtgg tgttcttata gatgaatata ttcctaaaac tataaatcca aaaaataatc
    11881 ttgtaaatgc cacttgttct gatgccaatc catttcccat taaaaacttt ccaaataatc
    11941 caagtgtagc ccataataac cctgctattg ctataaatac atacccttta cttttttctc
    12001 ccattttaag ttcccttcta tttttattta gtcaatttac actattcttt ttaagcaaaa
    12061 tttatgccat cttaaaccaa tatattccga ctctaaaacc ctagtctttc caacaatact
    12121 aataaaactt caattttttt atatttattt ttcacttact ttaagttgat ttagacttat
    12181 tttaattgta aaatgagtta tatataactt atttaaagtt aaaatagact gaaatttata
    12241 tataaatgtc aaattttaag tatattaatc aattaaactt attttaatat atcattaaaa
    12301 ggttaggagg aaaatagaaa aatgaattta aatttagaat tagatttgga attttacaaa
    12361 aaaatattag aagcttccca cgacgaaata tgtgtctctg atgataaagg aataataata
    12421 tattgtaata aagcatttga agaaaattat ggtctgaaaa aagaagatat tttaggaaaa
    12481 aatgtttctt ttttagaaga ctctggatat tcaacaaaaa gccccatacc agttgtatta
    12541 aaaacaaaat ctaaattttc actagaacaa gatactcaga caggaaaaaa gctcatcata
    12601 actgctaccc ctatatttga tgaaaatgga aacttagaat ttactgttga aaattgtaga
    12661 gatataactg aacttaataa tataaaaaat aaacttgaag acacaaaaaa acaagtaaaa
    12721 aaatataaat ctgaagtaga aacactatac agaactgctc ttagaattga agatactgta
    12781 attatggatg gaatagttat gagaccaatc ataaacacag taaatcatgt ttctaaaacg
    12841 gatgtaagtg tacttttatt gggtgaatct ggaacaggaa aaagctcact tgctagatat
    12901 atacaccaca acagcaatag agctaatgga ccatttataa caataaattg tgcaactata
    12961 tctccacaac ttcttgaatc tgaacttttt ggatatactt caggtgcttt tacaggtgca
    13021 agtactaaag gtaaagttgg tctcgtagaa ttagctaatg gaggaactct ttttttagat
    13081 gaaataggag atataccaca aaatctacag gcaaaatttt tgcaattaat acaggataga
    13141 acttttactc cagttgggag tttaaaaaac aaaaatgtcg atataaggat tatatctgct
    13201 acaaatgctg atttagtttc aaaagtaaaa gaaaaaaaat ttagagaaga tttatactat
    13261 agattaaacg ttatagaaat aaaattgcct cctcttagag aaagaagaga taatctagtg
    13321 gaaattataa aatattattt taatagatac tcaagtgact tcaacctaaa taaaactatt
    13381 tccaaagaag ctatggagac tattgcaaat tatagattcc caggtaatat aagagaatta
    13441 caaaatataa ttcaaaaaat tttacttact tgtacagata atcatataac aatacataac
    13501 ttaccaaaca ttcttacaaa aaatataaat atcaccaata atggaaataa aacacatata
    13561 tcacaaatta ataaagttat tacacctgac tcaaaatcta ttaattataa aaataaaaat
    13621 tttgatacct taattaaaga gtacgaaaaa aatataattt tggatgctta tgaaaaattt
    13681 ggtagttctt ataaagtagc caaacacctt gaaatttcac aatctaaagc caataggttg
    13741 attagaaagt atactaatac ttaattttaa atttgataaa taaaaactta acactattta
    13801 catattgtaa atttggctat ttattggtat tatgaatatg gttgaaatta tttaaataaa
    13861 tcctattgac ttagtttcat atatttacca ttaaactttt atcataaata tcatatcata
    13921 ataatatatt taataagtct aataaactat acgaggtgta aatatgttaa tgaataccca
    13981 ttttattatg gcaaaatcaa tacttgataa tatagatgaa aataaaacat tctttataag
    14041 cgaaaaaaat tttatatatg gtaatataaa gcctgatgct ttttctaaat ataaattaaa
    14101 aaaacattat ttagatgagt catttaatat gattgtttct aaaataaact atctatgtag
    14161 tcttaatata gatagtcttt ctaaatactt ttcagtaagt agacttagtc aggaactagg
    14221 agtcatatgc cattttttat gtgatttttt ctgtgtagct cacagtgaac gttgggaatt
    14281 taaacacagt ttaaataaac atgttactta tgaaagagaa cttggagcat ttgcaaaaga
    14341 tgttgattta tctaaaatta aatgtggtac tataaatagt agctttgaga agttttttac
    14401 taaactttat agtgactaca aaaagaaaac tgattatatg aatgatttaa aattttcagc
    14461 ttatacttgt aatagtgtca taaattatat acttgactct atcttgtcta atacaataaa
    14521 atcatatcat atagcaaatt gtggttaatt taagaatttt taaacttaca attgaatata
    14581 attaaaatga ctgctctata gaatataatt ttataattct ctagaacagt cattttttat
    14641 attaactatt tatctccatt ataacatcta tagcactttt tacactatct actacataat
    14701 ttgcactttg ctttacatct tcccttgcct tagacattgc aaaactatat ttaaatcctt
    14761 ctaacataca aatatcattt ggagaatccc caatagtagc aacttcatct ggacttacat
    14821 ttaaagtttt tattacatgc tctatacttc ccctttttga cacactttta ggaactatat
    14881 ttaaagtata tgttccagaa aaatatattt ctaatacttc actaaactcg ctttttagat
    14941 aaagttctat tctggataat tcctccaaat tttcagaaca tactcctata tttcctataa
    15001 ggttatcaaa actacttctt ttttcaaaat ctgggtcaac tataaaatca ctagaataat
    15061 gtctaaaagc agtggtatca ctacccacaa ttacatcacc cttatattta aaataaagtt
    15121 gtgcatcact tgactcaaat ctatcaatga cttttttaac taaattatca tctatcgttc
    15181 ttttaaaaat aacattataa tctttatcat atataaatga accattttca catatataat
    15241 atccattcat atctaaactc tcttcgataa tattacgagc ttgtttaaaa attctacctg
    15301 tagcaacatt aaactgtact cctttttttt ctatttcttc aattgctacc atgtcctctt
    15361 ttgttatagt tccattttca tataaagttc catctaaatc acaaaatata tgttttatca
    15421 taaattaaaa gcctccaaaa acaattttat attatactaa tattttaatc aaaataactt
    15481 aaagcttttc taattgtatt ctcaattcgt taagtaaatc tctagtagct cttaatctta
    15541 tctttcttct atctccatta aatatatttt tccttactat tgtttttcca tttatatata
    15601 ttgcagtata aactagtcct acaggcttct ctttagttcc tccaccaggt ccagctactc
    15661 cagttgtaga tatacctata ttagctttaa gtcctttagc aacgccctca gccatttcta
    15721 ttgctgtttg ctcacttact gctccaaact cttccaaagt ttctcttttt actcctagtc
    15781 ttttcatttt agaatcatta gaatatgcta cacaaccttc aagaaataca gaggatatac
    15841 ctggatagtt tataagagat gaagaaacca aacctccagt acaagattca gccactgcta
    15901 ttgttagatt tttttctacg agtaacttag aaacagcatc ttctaatgca gtctcccctt
    15961 ctgatactga aatatctcct tcagtatata catatttgcc aactctgctt tttatttttt
    16021 cttctactgg ttttattaat ttaaaggctt ctccttcatt ttcagccttt gcagttattc
    16081 ttattgtaac ttctaattct tttgcatata aagcaactgt tggattagtc tgctcattaa
    16141 ttatatcgag aatttctaat tctagatttg actcacctat tccatataat cttaaagtct
    16201 tagatactag catttcattt gtaaattgtt gtaagtaagg tttgacactc tcattaaaca
    16261 tagctttcat ttctcttgga ggtccaggta atactattat aaatttgcca tcttttctta
    16321 aaattgcccc tggtgctgta ccattattat tttttagtac tatagcatct tttggaaagt
    16381 acacttgttt catattattt tcggccattt tttttcctgt tttaacaaag aaagattcca
    16441 aaacctctag tgatggttta tgtagttcta atttctgacc aaagtattct gccgcagttt
    16501 cttttgtcat atcatctcca gttggtccaa gaccacctgt agtaattaca aaatcacttc
    16561 tctttaggct ttcagcaaaa cattctaaaa gtctttgttt attatctcca acagtactct
    16621 gatggtatac ttctattccc aatgatgcaa gttctttagc taaaaattga gaatttgtat
    16681 ttactatatc tcctaataat atttctgttc ctacacttat tatttcagcc ttcaaaattt
    16741 atcactcctt gatttatagc cattaagact agtcttaagt tcataacaat aattttagca
    16801 aaaataataa cataaataaa gttaaaaaat catttaaaca tatctttaaa ctagagtact
    16861 gctctaaaac cacacataat acaaacttcg tatatagtgg tactttcatc gtcaactgtt
    16921 aattcatata attaagtatg taaataaaaa atttacttta aaataataaa aatcaaaata
    16981 ggagtgtata atatgaaaat agcagttata atgggtggaa tatcatcaga gagagaagtt
    17041 tctttaaatt caggaaaaga gatatataat aaccttgata aaaataaata cgaagttgta
    17101 aaagtaatta tagatgataa aaaagatata tttacaaaga taccagaaga tatagatttt
    17161 gcaatacttg ctttacatgg aaaatttggt gaagatggat gtatacaatc aatacttgaa
    17221 actatggata taccatattc tggatgtgga cctctttgca gtgggatgtg catggataaa
    17281 aacataacta aaaaaatgct tagagattca aatctaccta ctgctccatg ggttctagta
    17341 aaatcagttg atgaaataga ctatgatgaa atagataata taggctaccc agtatttata
    17401 aaaccaaact caggaggttc aagtgtagcc acattcttta ttcattctaa ggatgaagtt
    17461 ggagaagcag ttagaaaagg tctggaagtt gatgaatttg taatgattga aaaatatata
    17521 ccaggtggag aatatacttc attcatatta aatggagaag tattccctac aatctctata
    17581 aaatctgatt caggattctt tgattatgaa gctaaatact cagttgaaaa aggtgcaaaa
    17641 gaggaagttg tttacttaga tgaagaactt caaaaaagag taaacgaaat ttctgagact
    17701 tgttggaaaa tttttaattg caaagcatat gtaagagttg atatgattat aagtgaaggt
    17761 attccatatg ttcttgaact aaatactctt cctggaatga ctcaaactag tttaattcct
    17821 agaagtgctg ctgctagagg tattaagtac agtgaactat tagataaact tatagaatac
    17881 tctttaaatt agatgaaata atttgaggtg gtaatttgat attttcaaat ctaaaattaa
    17941 atgataatga accaatttat atacaaatta aaaattatat aagtgatatg atatcaaaag
    18001 gactaattcc agacaatagt aagctccctt ctacaaggga gcttagtcag cttttacaag
    18061 ttagccgaaa ttctgtagtt ttgacttatg aagaattaaa atcagacgga ttaatctact
    18121 ctatttcagg aaaaggtaca tttgttaaat caaagaataa gtcttcaaat actacatggt
    18181 cattaaactg ggattattta gagaatacat actctaaaaa agccagtgaa ttggatatta
    18241 taaaaagtga aattccttgg agttctgacc taatttcctt taaaagtatt tctccagacg
    18301 gagatttatt cgacatggaa gaattaaaaa aatccttttt aaatagaatt tcactagaag
    18361 gacataaatt actcaactat ggatatgcac aaggttataa gccccttatt gactatttac
    18421 taaagtatat gacaaacaag ggtgttgata taagtaataa agatatatta ataacaaatg
    18481 gattcacaga gggttttgat attgttatgt catcttttac ccaagaaaaa gactacataa
    18541 tatgtgaaaa tcctactcat aatacagcta taaaaatcat gaaatctcat aatctcaata
    18601 tcttaggtgt agatataaat gaagatggtt tggacttcga tatgctagaa tacaacttaa
    18661 ttaagtacaa aaataaaata aagtttgcat acattacacc ctcttatcac aatcctacag
    18721 ggattgtaat gactcctgaa aatagataca aattttataa tcttatgaaa aaatacaata
    18781 tagcaattat agaggatgga tttaatgaag aattactcta caatagctct catatatttc
    18841 ctatctgttc cctagataat atgaataatg gcgttgtata tattggtagt ttttcaaaaa
    18901 ttttatttcc tggaatgaga attggatgga tatttgcaga taaaaaagta atcaataagc
    18961 tagaaagtgt aaaaaggtgt agaaacatac acgtatcctt tttagaccaa ggaattttat
    19021 ttgattactt aagtaatgga ggttttgaaa aatacattaa aaaaatacgt aaattctatg
    19081 gagacaaatt taactttgca tatgaatgtg taaaaaaaca tataaaaaca gaatacatct
    19141 tgggtgatgg tggtttgcat atatttataa aactaaaaaa tatagatact agagttctat
    19201 tagaaaaatg ttatgaaaaa aatgttatat ttatgcctgg agatttattt tttacagata
    19261 actctggttt tgatacttta agacttggat tttctaggtt gtctttcgaa gacattgaaa
    19321 ttggaataaa aataattgga gaaactataa atgaaatgtt ctaggttaac taaatattgt
    19381 tagcctagtt tatttttatg atgtataata tatatagttt atatgaatct aatactgggg
    19441 gataatatga gtaaaaaatc aaaacgaaaa aaaattaata aattaaaatt aacgattgta
    19501 ataactttat tatttcttgc tttattatct atatatgaga tactattctc taatcccaac
    19561 aaaaaagata atgtaaagag ccaagaaact atgcaaaata aaaataatac aacagatgat
    19621 tctaaaaata atcaaagctc taccaaaaag caggaagata acaatatcaa gcctactttt
    19681 tcatattctt caataccaga taatataaaa aataaaatga ttggtaaatc tatgcctact
    19741 gatgagccta taagctttga tagtctatct tatttaaaac ttacatacta tggatttgat
    19801 gaaaaaactc atcaaggaga aatgatagta aacagtgagc tagctccaga agtagtggat
    19861 atttttaagg aattatacga aaaaaaatat ccaattgaaa aaatcaatct aatagatgac
    19921 tatgatgctg tagatgagaa atctatgtct gataataata cctcatcatt ttgttataga
    19981 actatagctg gtacaaatgt catttcaaat catggtaaag gtcgtgctat agatataaac
    20041 ccattacaga atccccaagt ttctggaaat gatgttactc ctaaagtaag tacagtttat
    20101 gctgataggt ctagcacaaa atttggaatg ataagaaaag gtgatgattg ttataatgct
    20161 tttgtaagca gaggttggag ttggggtgga tattggaaaa acccagatta tcaacatttt
    20221 gaaaaataat agattagaaa taaaggtgaa ttatatgaat ttaatgacat tggaaaacat
    20281 aagtaaaagc tattctgaaa aaaaattact tgaaaatata tctcttggaa taaatgaagg
    20341 agaaaaaata ggacttattg gtgtaaatgg tactggtaaa tctaccttac tcaaaattat
    20401 tgcaggagtt gaagaaagtg aaactggcaa tataacaaaa gctaatggta ttcggataga
    20461 atatctacct caaaatccaa cttacaatga gaattcaact gtacttgaac aagtatttaa
    20521 tggaacttct gaagaattaa agctacttgg tgaatatcaa gaaattctag aagaacttaa
    20581 ttcaaacttt acagaggaat taaataaaaa attattatcg gtacacgaga aaatagatgc
    20641 cttaaatctt tgggatttag aaagtgaagc taaatcagta cttacaaagc ttggtataaa
    20701 tgactttaat caaaaaatag tagaactttc tggtggtcaa agaaagagag tctctctggc
    20761 atcagccctt attacacctt gcgagttttt gatactagat gagcctacaa accacctaga
    20821 caatgatact atagattggc ttgaagaata tcttaattca agaaaaggtt cattgcttat
    20881 gataactcat gatagatact ttttagatcg tgttacaaat agaattattg aactagacaa
    20941 gggtagatta tttagttatg atgggaatta ttcaatattt ttggaaaaga aaatggaaag
    21001 acttgcgtta gaagcaagta ttgaagacaa aagacaaaat ttaataagaa cagaacttgc
    21061 ttgggtaaga cgaggagctc aggctcgtag cactaaacaa aaagctcgtt tacaaagatt
    21121 tgacgaactt ataaataggg actcttttaa acctgatgaa aatctcgata tatctgttgc
    21181 atcaagtaga cttggaaata aaataataga aatacacaat atatctaaat catttgaagg
    21241 aaatactgtt atagataatt tagaatatac tttaactcgt acagatagaa ttggcataat
    21301 aggaaaaaat ggtatgggta aatcgaccct tataaacata cttaatggag aaatagaacc
    21361 tgacagtgga tatatttcaa ttggagaaac agttaaaata ggttgttttt ctcaagacga
    21421 ctctcatatg aatattgatt aaaaaatagc tacgcacact cgtgacttta gtcatgaatt
    21481 agtagctaaa atagtcagca tatagggaaa cttatatgta gagataggat aacacctatc
    21541 caatactact tgaattgctg gaaaccccta aagctaacca aactaaaaca taaggatgaa
    21601 ataaacctaa gtgtgaaagt tgcgaaagta gaaaaaattg gttagatggt ataaggttaa
    21661 atcctaagta ctttaaaatg ggcaatcagc agg
//
LOCUS       DAMJVM010000058        21626 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.060, whole genome shotgun sequence.
ACCESSION   DAMJVM010000058 DAMJVM010000000
VERSION     DAMJVM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21626
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.060"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            18..1298
                     /locus_tag="PC523_002330"
     CDS             18..1298
                     /locus_tag="PC523_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDF2475845.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPTEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKILNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIKNNICSA
                     TAIRTSLKENINFFHLKNVIPEKTYNILNEKIDNGFLPMFDDLFFDTLKTIILRDMDT
                     LKDYFEVNEGIENRIYRDIFTSEFLHELQLSIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREVLKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKSTNIYNSVYYKNNKDLVKGPMDFYTHPIYVK"
     gene            complement(1561..2952)
                     /locus_tag="PC523_002331"
     CDS             complement(1561..2952)
                     /locus_tag="PC523_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDF2475846.1"
                     /translation="MYKLVDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEVINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLVPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(2963..4183)
                     /locus_tag="PC523_002332"
     CDS             complement(2963..4183)
                     /locus_tag="PC523_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HDF2475847.1"
                     /translation="MSKIVVNQDKITDLKRVLEICPFGAIEEKSGVVEISAGCKMCKL
                     CVKSGPKGAFEFVESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCNNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFINKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSVPEKIENATGKVTLCKIEKKDLSSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMMYKLANKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDDNQKYMASAAK"
     gene            complement(4197..4985)
                     /locus_tag="PC523_002333"
     CDS             complement(4197..4985)
                     /locus_tag="PC523_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2475848.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKQEKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEAKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLDTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSSALVNKLEELKLV"
     gene            complement(5002..6276)
                     /gene="larA"
                     /locus_tag="PC523_002334"
     CDS             complement(5002..6276)
                     /gene="larA"
                     /locus_tag="PC523_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HDF2475849.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRKINPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHVSTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSILPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSEVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMIAACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTVPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEKATSIVGKDSKVVVIPDGVSVIVK"
     gene            complement(6394..7107)
                     /locus_tag="PC523_002335"
     CDS             complement(6394..7107)
                     /locus_tag="PC523_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF2475850.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLGDILEL
                     RIILEIEIAKLASKRITSSEAIELKNIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEK"
     gene            7232..8491
                     /locus_tag="PC523_002336"
     CDS             7232..8491
                     /locus_tag="PC523_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HDF2475851.1"
                     /translation="MKKNKIIAFFAPAIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNRQISKHEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNESLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSAPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGILAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVIIIYLISAVFIAKK
                     HMKALSTNGKLMRYKNTFFIKKFSRKK"
     gene            8758..9324
                     /locus_tag="PC523_002337"
     CDS             8758..9324
                     /locus_tag="PC523_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2475852.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKHLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(9523..10728)
                     /locus_tag="PC523_002338"
     CDS             complement(9523..10728)
                     /locus_tag="PC523_002338"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDF2475853.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNASKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNDVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDDNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRNSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILGCLDKYVDDYDIIDDEELAIFVSAKSEHEIDRFLEDVKRNNYSE
                     SVKKLLKID"
     regulatory      complement(10831..10937)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(11029..11397)
                     /locus_tag="PC523_002339"
     CDS             complement(11029..11397)
                     /locus_tag="PC523_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HDF2475854.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDAKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(11449..12726)
                     /gene="obgE"
                     /locus_tag="PC523_002340"
     CDS             complement(11449..12726)
                     /gene="obgE"
                     /locus_tag="PC523_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDF2475855.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELESRGYTVFKMSAATRQGVDDIIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(12837..13127)
                     /gene="rpmA"
                     /locus_tag="PC523_002341"
     CDS             complement(12837..13127)
                     /gene="rpmA"
                     /locus_tag="PC523_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDF2475856.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(13141..13458)
                     /locus_tag="PC523_002342"
     CDS             complement(13141..13458)
                     /locus_tag="PC523_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDF2475857.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECMVKDTHIKDAQLLLNHFQLAVEEIKREYPKNIKI
                     LKK"
     gene            complement(13473..13784)
                     /gene="rplU"
                     /locus_tag="PC523_002343"
     CDS             complement(13473..13784)
                     /gene="rplU"
                     /locus_tag="PC523_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDF2475858.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(13885..15306)
                     /locus_tag="PC523_002344"
     CDS             complement(13885..15306)
                     /locus_tag="PC523_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HDF2475859.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNVYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSAIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCNHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYRTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     NEVLNILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDNIEKEIMRISEHTVYKNIIVEFNDFIFEQIKGYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(15324..16025)
                     /locus_tag="PC523_002345"
     CDS             complement(15324..16025)
                     /locus_tag="PC523_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HDF2475860.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPEGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKINLNTNILIPKILEIFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(16027..17871)
                     /locus_tag="PC523_002346"
     CDS             complement(16027..17871)
                     /locus_tag="PC523_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HDF2475861.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            18190..19383
                     /locus_tag="PC523_002347"
     CDS             18190..19383
                     /locus_tag="PC523_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HDF2475862.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDADVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(19595..20159)
                     /locus_tag="PC523_002348"
                     /pseudo
     CDS             complement(19595..20159)
                     /locus_tag="PC523_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422542.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20360..21151)
                     /locus_tag="PC523_002349"
     CDS             complement(20360..21151)
                     /locus_tag="PC523_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475863.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLERHKSIVMWFKKVLGYISIIAFLIS
                     GFMWYYNNNLQHNMYNLGKELDGEIKQLAERHDMTKIDEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYDREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFALGLLCIIAYFILRFKISLIKNNN"
     gene            complement(21155..21484)
                     /locus_tag="PC523_002350"
     CDS             complement(21155..21484)
                     /locus_tag="PC523_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2475864.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
BASE COUNT         6976 a         3825 c         2387 g         8438 t
ORIGIN      
        1 gataaaagga gtattttatg aacatacttg gattaatagt tgaatataac ccatttcata
       61 atggacacat atatcattta tctaaatcta tagaaaagac aaatgcaaca catactgtag
      121 caataatgag tggaaatttt ctacaaagag gcgaaccagc tttatttgat aaatacacta
      181 gagcaaaaat tgctgtcgaa aatggtgtcg acttagttat agaattacca actttatttg
      241 cttgccaaag tgctgaaatt ttttctcatg gtgctataac aattttaaat tcactaaact
      301 gcataaattc tatatgtttt ggcagtgaag aaggaaatac agaaattctt tataatatag
      361 ctgaaatttt agcaaatgaa ccaactgaat ttaagttact cttaaaaaaa tatttagatg
      421 atggtctgtt atttccaacg gctagagcta atgcactttt tgattatata catcaagaca
      481 aaaaaaataa ttttatacaa aattattcaa aggaacaatt atcaaagata ttaaattcat
      541 caaacaacat actgggtata gaatatataa aaaacctcat ccatttaaac agtagtataa
      601 aaccatttac tatatccagg gttcaatcag aatataattc tgaagaaatt aagaataata
      661 tttgttctgc tactgctata agaacttcac ttaaagaaaa tataaacttt tttcatttaa
      721 aaaatgttat tccagaaaaa acttataata tcttaaatga aaaaatagat aatggttttt
      781 tacctatgtt tgatgacttg ttctttgata ctttaaaaac aattatactt agagatatgg
      841 acactttaaa agattatttt gaagtaaatg aaggcataga aaacagaatc tacagggata
      901 tttttacgtc tgaattttta catgaactac aattatcgat taaatccaag cgatacactt
      961 taaccaaaat caaacgaact ctaaataata tattgttggg tataaaaaaa agcgacatgc
     1021 aacttataaa aaatatagat actatacctt atataagaat acttgctttt aatgacaaag
     1081 gaagagaagt actaaaagag ataaaaaata aatctgaaat aaaaataata aacaaatttt
     1141 caaatatatc attttcatta gatgatacga tttttaaaac actcataagt tatgatataa
     1201 aatctacaaa tatatataat tcagtatatt ataaaaacaa taaagattta gtaaaaggcc
     1261 ctatggattt ttatactcat ccaatatatg taaaataatt ttttaaaata actatattca
     1321 taaaaactat tttattaact ctttattata taagatacaa taatatcact ttatctatgt
     1381 acacatgttt ttaggatgac aacatcaact taaatattat gttactttct aaatttaagt
     1441 aaatattgcc atcctaaaac ttttaaaatt tagcttcaaa tttaaaggta aggtttaaaa
     1501 tattacattt aaatctaatt taatattttt tgttttacaa acattattaa attaatattt
     1561 ctaatatatt tttcctgggt ttaaaatatt tttagggtca aaagctaact ttatattttt
     1621 aattaacatc atataagcat ctgaatttga ttcatgtaag tactcttttt tagcatatcc
     1681 tataccatgc tcaccagaaa cttgaccact aagttctcta gattttttat acatatagtc
     1741 aaaagtttct tttaatctta ttttccatgt attatcatcc ataccatctt ttaaaatata
     1801 tatgtgtaga tttccatctc ctgcatgacc aaaacttttt attcttattt ttaatttgtc
     1861 ttgaagctcg tgagtgtatc ttataaactc tgctatctta tctcttggaa caacaacatc
     1921 acattcatcc atttgtgtcg tagatgcctt tatagcctct aaaaatgcac ctctggctga
     1981 ccatatagaa tcatttcttt cttgtgtatc tgatataaat acatctaatg caccattttc
     2041 tagacataaa ttagctactt tttcatattc tttttctata tcttcagtac tatttccatc
     2101 aaatgtaagt aaaagataag catctgatgt attatctgga aactttttcc ctaaaaactc
     2161 ttctgctgcc agtattacat ctctttccat aaattctata gctgttggta ttgattttga
     2221 ttttattatt ttaggaacag tttctattgc cattgataaa tctggaaatg gtacaagtag
     2281 acttatactt ttctttggta aaggaagcaa ttttaatata gctttagtaa ctattcctaa
     2341 agtaccttct gaacctacta ataagtcctt tatactatat ccagaactat ttttaaccac
     2401 ctttccacct acatttataa cttctccatt tggtagtaca acttcaagac ctcttacata
     2461 atctcttgta acgccatatt ttactgccct cataccacct gcattagtgt ttatattacc
     2521 tgcaattgta gcactttttt cacctgggtc aggtgggtaa aataaatctc tatcttgaac
     2581 atattgacct attgtcatca acaaaacacc tggttctact gtcaatgtta aattttcata
     2641 atcaacctca agtattttat tcattttaca tagattaatc attattccac catttatggc
     2701 aacagcagca cctacaagac cagtaccttg acctcttggt gtgactggta tattattttt
     2761 atatgcgtat ttcattattt tagatacttg ttcagtttca agcacattta ctaaaacttc
     2821 agggtacttc tcaatccctc caagttcatc atgtgaaaaa tcttcattta tatcacttcc
     2881 aactaataca ttttcttctc cacaagtatc tattaaaaaa tctatatctt ttttatcgac
     2941 taatttatac atttctatcc ccctatttag ctgctgatgc catatatttt tgattatcat
     3001 ctttgttatt ttctattttt tcaatcaatt taggaataat atcataaata tcacctatta
     3061 aagctagatg tgctacatca agtataggcg ccttatcatc tttatttatt gctactatat
     3121 agtcagcccc ctgcattcct gcaacaaatt gaactgctcc agatacacca caagttatta
     3181 tcagtttagg ttttacagtt ctaccgctaa gacctatttg ttttttagca tctatccagc
     3241 ctgcttctat tacaggtctt gttcctgcca cttgagcatt taacttatta gctaacttat
     3301 acatcatctc catatcttct tgttttttta ttgctctact tgccacaact atcacttctg
     3361 cttcttccag accaacttct ttattttttt cttttacact tagtacttta attttagaac
     3421 ttaggtcttt tttttctatt ttacataaag ttacctttcc tgtggcattc tctatttttt
     3481 ctggtactga aaatatttta tatcttacag ttgcaaattg tggtctatta ttaggtgtat
     3541 ttatatgagc cattatattt ccaccaaatg ctggtcttat ttggtctaaa tcagtatttg
     3601 attgaatatc aagtattgta caatcggctg taagacctgt tctaaatcta gcagcaagtc
     3661 ttggtgctaa tgacctacct aatgtagttc caccaactaa cactatcgtt ggttttattt
     3721 tatttataaa gttttctatt gcttttgaat atggttctat cctaaaatct ttaaattcat
     3781 cttcatcata tacaaatact tcatccacac cataagaaat caaattatta catttatctt
     3841 ttatgtcttt tccaacaaat acacaataca caggttgttt tatttttgat gccatctcac
     3901 gagctttacc tataagctca tatgttacag gatgtatatt tccattatga tgttcaacat
     3961 atactgcgat tcccctccat tcatctttat taatttgtac tttactactc tctacaaatt
     4021 caaatgcacc ttttggtcca cttttgacac acaatttaca catcttacag ccagcactta
     4081 tttctactac acctgacttt tcttctatag ctccaaaagg acatatttct aatactctct
     4141 ttaagtcagt tattttatct tgatttacaa caatctttga catttattaa tcccccttac
     4201 accaacttta attcctctaa tttatttaca agtgcagaac ttagttcatc tgcatttcct
     4261 cttacaatat ttttatctgt attcggcttt ggaggaaata ttcttacaac ttgagtagga
     4321 gaaccattga gtccatagtt attttcatct ttatcttcaa agtcatctaa gctatataca
     4381 ggtatttctc tatctttagt atcaagtttt aatttaaatg aaggtagtct tggttgaaaa
     4441 atatcttttt ctacagttat taaacatgga tattcaattt cacatacttg taaatcattt
     4501 ggcatatcta tttcaacaac tatactttca tctttagctt caattaattt tgtaacatta
     4561 gtaacatgag gtatatctaa aaactcagct acttcaggtc ctacctgtgc tgtatcacca
     4621 tcagtagttt gttttccaca aattattagg tctacctttc ccatcttctt aataccctgt
     4681 gatatggtat aggaagtagc caatacatct gcccctccaa atctcctatc acttattaat
     4741 gctccttcat cagctcccat cataaaactt tcttcaatta ctttctttgc ctgaggagga
     4801 cccatgctaa gtacttttag attagctttc ttctcttgct ttattctaag agctgtttct
     4861 aaagcatata ggtcataggg attcattttt gaatctactc catctctttt tagtactcct
     4921 gtagtttcat caacttctac tgaagtcgtt ccaggaactt gtttaataca tgtcaaaatt
     4981 tccataaagt acactccttt tttatttaac tataactgat actccatcag gtataactac
     5041 taccttgcta tccttaccaa ctatactagt tgccttttct aaagcttctt taaaattaaa
     5101 tgcatgtttc atatgcattt tttttatcat ttcaggatta cacttatctg ttaccattat
     5161 cacagtatat ttagataata ttctcgctag tatctgaaat tcccattggt ctggaaccgt
     5221 atctactcta tcaacacttc taattttatc taaaagttgt tttggagaat ctgcgtttgc
     5281 tacattttca taaaaagatt gaccaccatg accatcatta catgctgcaa tcattattat
     5341 tactccacca tctttgcagg tagcttcagc cgctgtcatc cctttaacag attgataaat
     5401 attttggtcc agtggatatc caccatttgt agttacaaca atatcagatt ttatagaatt
     5461 tacttctgat agctctgtta caaatctaca tcctttagtg tgtgcttttt cgacatcacc
     5521 agcaaaagaa gcaattattt ttttatcttt atctattaca acattcaata tgaatgctaa
     5581 ttttgcaact ttagctgcat ataccatatc ctcatgaata ggattattgt tcaatttccc
     5641 agttcttgaa ttatcactgt ctataaagtc actacaatgg ttgtacatta tagtctttgc
     5701 agatgctatt cctggaagta tgctttttct cccacctgaa aaacctgcaa agaaatgagg
     5761 ttctataaaa ccttctgcta ttaaaagttc tgcctcatat gctaatttgt ttatatataa
     5821 atctccacct gaaggtaata ttcctacttt gcacatagat ttatcatcag tagatacatg
     5881 cataactata tcttcattat ctactatttc ttctcccatt ttataaatta gctcttccct
     5941 tgtacttggc ctgtgaaatc cagttgccac tataatcttc acatcaattt ctggattaat
     6001 cttcctaatc cttcttaata taattggcat agttattcta cttggtacag gtcttgtatg
     6061 gtcacttgta attatgacta cattcttttt ccccttaact agttcttcca gagaattaga
     6121 gcctatagga ttattcatag attcttcaac tatctcttct tgggtcttat gttctatata
     6181 attatccgat tttgactcca aaacaccgag aaaacgttca tcaggtattt ctaaaatcat
     6241 tccttgtttt gaatacggta cttttaactt taccattttt aatctcccat tataatagac
     6301 tataaaattg gtatgtggtc ataccagttt tacatttata tgatagtacc attatctaat
     6361 attttcaaga ataatttatt ttataacaaa cttttatttt tcattttttc tataattttt
     6421 acttataaaa tccatatgtt cctttgcttt ctcacatgca agttctacat cattttctac
     6481 aactgcattg tatatagctt catgctgttt aagtaatatg ttttcattga aaggttctgc
     6541 gattattttt tctcttgcat cttctataaa tccatcaaat aattttgatg ctgtcataaa
     6601 aagactttga attaaataat ttttacttaa tgttgctagt ttactgtgaa atttttggtc
     6661 taataaaacc agtactcgat tattatcttt tttattagtt tctaccctca tttcatctat
     6721 tatatttttc aattctatag cttctgaaga agttattctc tttgaagcaa gttttgcaat
     6781 ctcaatttct aggattattc taagctctaa tatatctcca agtgtaccat tattaagttt
     6841 aaaaatcata gataaaggtt ctattaaagt tttttctata tttgtacata taaaatttcc
     6901 ttccccttgt ctgctttcta tcactcccat agtctctaat actctaatag cttctctgat
     6961 ggaagttcta ctcacattca tttgttcaga tagttctctt tctgatagta atttatcccc
     7021 tttttttaac tccccattta gtattttata ttgtatttgc tcaattactt gttcataaac
     7081 ttttttacta gctatactat taaacatttt tccctccagt atgataatcg ttgttgtaac
     7141 atatatctat tatatatctt aagccattac attcatatat ttttattact tttaatataa
     7201 tatcttataa ctcaaattta caggtgatat tatgaaaaaa aataaaatta ttgctttttt
     7261 tgctccagct attatagttg gagttctagt tttagggatt atcctatacc caaatgaagc
     7321 tataaaatct gcaagagaag gcttctctat atggtctaat gttttagttc catcactact
     7381 accatttata attggagcaa acttaattgt tgatttaaaa atagtggata ttatagggtt
     7441 tattatcaat cccataacta gatttgtatt taatgtttca ggaaagagcg ctttagcttt
     7501 tgctatatct acagtatctg gttatccagt aggagtaagt ttagcttctg aatttaggag
     7561 taataggcaa atttctaagc atgaagcaca aagactagtg tctttttgtt ctacatcagg
     7621 acctttattc ataataggct cggttggtac aggtatgttc caaaattcaa acttaggtta
     7681 tttaatgatt ctttgtcatt atctagggac tatattggtt ggtcttttct ttagaaatta
     7741 tggtaatgaa agtcttccaa agacgaaatt agatttaaaa acaaacataa aaaatgtaat
     7801 caacattaga aattcaagtg ggaatggttt ttttgtttta tttggaaatg ctgtatttaa
     7861 cggagtaaat acattactta cagttggtgg atttgtaatt gttttttcag tggtatttaa
     7921 aatactctct ctttttaatg taatctcatt aatagcatca gttatatatt taccgctttc
     7981 atttttaggt gttagtaaag aactctgtca agcatttgta agtggtctat ttgaaattac
     8041 tataggatgt aataaagtat ctagtataac aagtgctcca gaaattctaa gagcatcttt
     8101 agctagcttt ttgataggat ttagtggatt atctattcta gctcagtgtt gtacatttct
     8161 atcaaaaaca gacatagatt taaaattata catactaagt aagtttgcac atggaatttt
     8221 agctgctatc tttatattta tactttatcc aattgcaaac tctgctgtat tagtttctag
     8281 ctttgctgat acatacaacg ttatatacaa taatttaatc tggttttatt atcttagcta
     8341 ttacgataca attcttcaaa ttgtaataat aatttatcta atctcagccg tatttattgc
     8401 aaaaaaacat atgaaagctt tatctacaaa tgggaaacta atgagatata aaaatacttt
     8461 tttcatcaag aaattttcac gaaaaaaata aaaataataa aactttacta aatacttcca
     8521 tttttttatt atatttgtca taattttaat aaccttttta attagtttaa caacatagtt
     8581 atttcatcac aatttaataa ttttattaga aatatttttc aaaaattctc cttaataaca
     8641 tatatcacac ttgtataagt ttactggcaa atttttccat tatataatat ttcatttata
     8701 ttatataata aataccgacc taaacttacc aaatcaaact aagaggagtg gttgaaaatg
     8761 gcaacaagac ctatagaaat agacgaatat aaaaagataa tggaactatt acatactggc
     8821 tttacttata gtgaaaatgg cgttgaaaaa agatttagaa aaaatcctaa agttgcattg
     8881 gcactaatgt tagaagctaa tttaggtcta agaatatcag atattttaag attaaagata
     8941 ggtaatttta aaggtaatat gttagaaata aatgaaaaaa agacaggcaa attacagtat
     9001 agaccagtta ataaaaatat aattgagtcc ataaatgaac atgcaagaaa gtataatctt
     9061 aaatcaaatg actacttagt aaatataaag acaaaagcta ttcagaaaca gttgaggata
     9121 atttgtaaac acttaaatct gtataatata agtacacata gctttagaaa gctctatgct
     9181 actactcaat ttgaaaagag taacaataat ttagagttgg ttaaggagct tttaaatcac
     9241 agctctgtgg ctactacaca gcgatatata agggtaactc agcaagctat taatgaggct
     9301 agtgaaaatt tctttattgg ttaaattata acgtatacat ttttctatta tcttattaat
     9361 tcaccagata taaaaaaaga gtgtcttatg aactaaaaag ttcataagac actctttttt
     9421 tataaagtca aatatatttt cattatttca acactggaaa agaggcttat ttctctattt
     9481 taaaacagct tcttaaattt tcataattcc tgcataaaaa tattaatcta tcttcaataa
     9541 ttttttaacc gattcactat aattatttct ttttacatct tccaaaaatc tatcaatctc
     9601 atgttctgac tttgcactta caaaaatcgc taattcttca tcatctataa tatcataatc
     9661 atctacatat ttatctaaac atcctaaaat tccatcttta ttgttataac caccaaatct
     9721 aactactgca ctacactcat cttctataag tccacttaaa tctaattttc cttttgaaaa
     9781 agactttata tttgaatttc tattatctat tatatctata atatctgcat aaacagcact
     9841 tcctgttgca cctcttccag ctcccttacc tacaaaagaa agttcaccta ctgaatcacc
     9901 tactaaaata actacgttga actcattatc tatctttgaa agcatattat tatcatctac
     9961 taatgctggt ctaacaaatc cacttacttc tcctgattca tacttacttc tagcaaccaa
    10021 ttttatctta caatttaatt tttttgcata ctctatatct ttttcatcta ttgaagttat
    10081 tccttctaat aataaatctt tccaatatac tatttttcca taagataaag tagacaatat
    10141 agataacttt ctagcactat catatcctaa aacatctgca tctggattaa cttctgcaaa
    10201 tcccaactct tgtgcttgtt ttaaagccac atcatatggt aaattttcat catacatctt
    10261 tgataatata aaattacaag tcccatttaa aatagcattt atactcttta tattattgcc
    10321 ttccagtgat tctatcaatg gttttaaaac tggaatacca cctgctacag atgcttcaaa
    10381 cttgatgcta acatcattta agttagctag ttctataagg tcgctacctt tttcagccag
    10441 taaatcttta ttagctgtta ctacatgtat ttttttattt aatgctcttg ttacataatc
    10501 aaaagctgga tttaatcctc caattacttc aactagtata tcaatatcct tgtcaaatat
    10561 atcctctata tcagtagtta tcttatcaaa atacttctta tacttgtgtt tttctttatt
    10621 tttaactaaa atcccaacaa cttctatatc tctttttgaa gcattatttt ctattaaatc
    10681 aattaatcca cttcctactg ttccataccc taatattgct atcttcactg taacataccc
    10741 ccatcatttt gattatatta aattatttat atatataaat ataccaatta aaaaagcttg
    10801 agtataaact caagccatat gacgggttta tactcatctt tcagcataaa tgctgcagga
    10861 tttagcacca atgtataatt aaatatactg gttgccgggc ttcacagggc cagtccctct
    10921 gccactcttg ataagattgc taatttaaat ttatgtttaa tagtataata caatttctcc
    10981 caattgtcac tattttttta acttatttct ttaaaaggat ataaactatt atctaacagc
    11041 tcttttagtt ggtttaccct tttttgtaac attattattt ttagtctttg ctttcgcctg
    11101 ttcatgtcct ggaaaaagta ttttaggctc ttcaatgttt ctcttgtaaa tagtaaattt
    11161 aaaaccaata gcctgcacaa attcagctct taaagcttca catactgcat tagcagtttc
    11221 tttagcatca agacctgcat tttctaatat agtaactttt accaattccc tagttcttaa
    11281 cataccatct aattgctcta agaagctctc tgtaacacct tctttaccaa tttgtgtagt
    11341 aggctttaaa gtatttgcca aagatcttaa atacgccctt tgtttccctt ttaacatatt
    11401 ctcacctcta taattctata cttaaattat ctgtaatcta tatttaaatt aattgtaaaa
    11461 ctcaaattct aattcataaa ttttaactac atctccatct tctattccca tttctctaag
    11521 cctgtcaaaa acaccttgac tctccatagc tttttggaaa tattgaagtg attccatatc
    11581 atcaaaattt acagaataca ttattcttct aagtgcttta cctgtaacta catacacacc
    11641 atcttcaata tctatactta gctcttcttc agttccaata tcaagttctg gtctatacat
    11701 ttcttcttct gaaaccagct ccacatcttc tacttctttt agcattttag aaacatatgc
    11761 tattatatca tctacgcctt gacgagttgc tgctgacatt ttaaatactg tatatcctct
    11821 gctttctaat tcactcttaa acttctcaaa cttactttca tcttctaata tatcaaattt
    11881 atttgcaact acaacttgtg gtctctttga aagcttttca ttgtacaatt taagctcatc
    11941 atttatcttg tcaaaatctt caatagggtc tcttccttct aacccagata tatctacaat
    12001 atgtatcaat acctttgttc tctcaacatg tcttaaaaac tcatgaccta atcctatgcc
    12061 ttcagaagca ccctctataa ttccaggtat atctgcaagt acaaaactat ctccaaactt
    12121 agtttgaaca acacctagat ttggtgtaag tgttgtaaaa tgatagtttg caatttttgg
    12181 tttagcttta gttacaactg atagaaatgt tgatttgcct acatttggaa atcctaaaag
    12241 accgacatct gctatcattt taagttcaag agttatccat ctttcttctc catctgtacc
    12301 agattttgca aaagctggag cttgccttac tgcatttgca aagtgttgat tacctttacc
    12361 acctatacca cctttggcaa ctatagctgt atctccttct ttttttaaat ctgctagtac
    12421 aagacctgta gcctcatctc ttattactgt acctgctggt acttttaata ctaaattttc
    12481 tccattttta ccagctctct tacctttgct tccgtcgcca ccattttgag cttgatattt
    12541 tttctggtac ttaaagtcca ttagagttct aagacctaaa tcaacttcaa atatgatgct
    12601 tgctccacga ccaccatctc caccatctgg accaccagct ggaacgtatt tttctcttct
    12661 aaatgctacc gaaccatttc caccatttcc agccttaaca aatattcttg ccttatctat
    12721 aaacaaattc ttcacctact taattaaaaa cttttaatta gcattaccaa aaataccatt
    12781 ataaattcat cagtattata agaaaaagga gtgcctcaac actcctttta tgtatattac
    12841 tcagctattg ctactggata tatactaact ttttttctct tcttgtcttt tctttcgaat
    12901 ttaacagtac catcaactaa tgcaaataaa gtatcatcgc taccttttcc aacattagtt
    12961 cctggatgta ttttagttcc tctttgtcta actattatac ttccagcagt tactaattga
    13021 ccatcgaatc tcttaacacc taatctttta gatatagaat ctctaccatt tttagaagaa
    13081 cctacccctt ttttagatgc taataattgt aaattcatgt ttaacatatg gagtcacccc
    13141 ctactttttt aaaattttta tattttttgg gtattcccgt ttaatttctt caaccgctaa
    13201 ttgaaaatga tttagtaata attgtgcgtc ttttatatgc gtatctttaa ccatacactc
    13261 tatatgaccc tcttctccta ctactttttc aaattcaatc ttttgtaatt tgtctaaaga
    13321 atttataact gcaatagcat tagatgtaac tgcagcacat actatatcat agcctatttc
    13381 agcaaaatca gcatgtccct ttaaacaaaa accttgtaac aagaaatcat cattataata
    13441 atatttaacc tttatcataa cttaaccaat tactaagcgt ttattttttc tacaactatc
    13501 ttagtgtatg attgacgatg tccttgtttt cttctgtaat ctttttttgc tttatacttg
    13561 aaaactataa ctttcttagc tttaccttgt tcaacaactt tagcttgaac agatgctcct
    13621 tctactactg gagaacctaa ttttaattct ccatctttag agcaagctaa tacttcgttt
    13681 aaagtaacaa catcacctgc gttagcttct aatttttcaa cgaataatac atcaccttca
    13741 gaaactttat attgctttcc accagtcttt actatagcgt acattaaata cacctcctcg
    13801 gcctcaaact cgccagtaca aggtacttca tttgaagatt taaaccttta ctgtgcggca
    13861 ttacaattat taattataaa atatttacat tttattgtca actattttat cgaaaataac
    13921 attggtttta ttgtgcttta atgtactatt tgcgtttaaa gaaatcttta tattatattt
    13981 ttcaccaatt ttatcaatta tatccatata atatcctttt atttgttcaa aaataaaatc
    14041 attaaactct actattatat ttttgtaaac agtgtgctca cttattctca taatttcttt
    14101 ttcaatatta tctagtatat aatgtacaga ttttattgtt tgttcaccat cacaacatgg
    14161 acaagacatt aaataatact tatcaatgga ttctttttct ctacgcctag caacttctac
    14221 taaaccaagc tttgtcatac ctaatacttc agcttttctt ttatctttat taaattcttc
    14281 ttctaggatg tttaaaactt cattttgatg cttcttttta tgcatgtcga taaaatcaat
    14341 tattattatt ccagctatat ctctaattct aagttgcctt acaatttctt ttgcagcctc
    14401 aagattcgtt ctataaacgg tttcgtcaag tttaccagtt ccaataaact tacctgtatt
    14461 tacatctata actgtaagag cttctgtttt ttcaattatt aaataacctc cactctttaa
    14521 ccatactttt ttatttaata atttttctat tttagattct attctgtata aatcaaatac
    14581 atctctattt tcctcaagac acagtttatc aacataagtc ttatctatac ctcttaaaat
    14641 agacttaagt tcagagtact tatcgtaact attggtaatt atagattcaa tatcatcatt
    14701 cacattatct tttacgtatt tagttgcaaa atctaaagac ttatataata atttcggacc
    14761 tataccaagt ttatattctt ttaaaatgtt ttcatacttt aattttaact cttctatatc
    14821 tttctttatt tcatcatgat tacaaccttg agcctcagtt cttattatta atcctagatt
    14881 ttccttattt actgccttag taattttttt taatctaaat cgctcttttt catcagtaat
    14941 tttatttgat attgtagttc tatcattgga aggtatatag acaatatacc ttccagaaat
    15001 actaatctca gtgtttaatt ttgcaccttt agttcctatt tcttctttat ttatctgaac
    15061 caaaatatcc tgaccagact ttatagcact tccctttttt aattgtaaat aagcaagttt
    15121 cttaaatcca atatctacaa aacaagcttc tattcctggt ataacttttt ttacgattcc
    15181 acgatataca ttggatacag tcttcttatt taaattgtct tcaacaaaaa gttctgttaa
    15241 tctttcatct tcaagtactg ctgttttctg agaccctatt agggactcaa taactatctt
    15301 tttcacaact tcacctctcg agcttacata ggagtcacta attccccatc ttctaataca
    15361 tataaatctc ttctcaatat atcaacatct aatgggtcaa tatcaagttc aaatatctct
    15421 aatatctttg gtataagtat attagtattt aaattaattt ttgagcctgt agctatagta
    15481 gcagtaagag taatatgttc atcttctaaa tttaaaacat caaaggttct tatcatgggt
    15541 cttatatcta cttcaaccat cttacctttt ttattctttt ttgttatgat tatttcttta
    15601 ttattcataa agtcaattac ttttcttttt acaaactctt tggtcatagg tcttttcaaa
    15661 tctatattaa acaaatattc tccataatct ataacagaag aaagagaagg tgtttgtcta
    15721 tcaatttctt ttgctttgat aaaatctatc ccctctggaa gttggattga tactttattt
    15781 aaaaattcct ctactgataa atcatcttct tctatctcaa tatctacata ctcaccctga
    15841 ctttctgttc ctaaagccaa agcattacca taactcatct tagggtgagg attaaatcct
    15901 tgagaatgtg ataagtttat ttcagctctt ctaaatgctc tttgtaataa tctctgaagg
    15961 tcaagatgtg atatatatat catatcgcct tcctttttaa attttactct tattatctta
    16021 ctcatattag caaagccccc ttcctatatc atgtatattt attccacagc cattgcagtt
    16081 atgtctacaa tcagaagtga ttttttcttt tttagcattt tcatcttctc taattaagaa
    16141 ttttttacta atacctacat ctatatggtc ccaaggaaat acctcttcat aatttctatt
    16201 tctatgtgca taaaaatcta tagaaagatt attcttttcc atagcttctt tccatatatc
    16261 taaattaaag tgctctgccc atccatcaaa tttagcgcct agtctaaacg catcatatat
    16321 cacttttcct attcttctat ctcctctagc tactactgct tccatcaaac tagtctttga
    16381 atcatggtaa ttgtatttta tattgttgtt tcttaattta tttactaaat gtctttgctt
    16441 attaatcact tcttctgtag tgtcttgacc atgccattga aaaggtgtaa atggctttgg
    16501 tacaaatgta gaagtactca ctgtaacact aaaacttctc tttagctttc ctccatttac
    16561 ttttctataa atatcaataa ctttataagc taatttagct ataccatcta aatcttcata
    16621 agtctcagtt ggtaaaccaa tcataaaata caacttaaca ctattccatc ccatctcaaa
    16681 agctctttct gttgcatttt ccaaatcttc ttccgtaaca cctttattta taacatctct
    16741 aagtctttga gttcctgctt ctggagcaaa agtaagccca gactttctta cttgctgtat
    16801 tttgtcagca atttccattg agaagttatc taatctaagg gatggaagtg aaataccaat
    16861 attattagat gcatattcat ccaccaaata atcagtaagc tctgataact tactataatc
    16921 actcgtacta agtgatgata atgatatttc atcatatcca gtattcttaa ctaatttatc
    16981 caatatttct ttaagtcttt tgacactctt ttctcttatt ggtctgtata tcatacctgc
    17041 ttgacagaat ctacatcctc tagtgcatcc tctaaataat tctagtacta ttctatcatg
    17101 aacagtatct atataaggta ctattagttt ttctggataa tctacagtct caacatcttt
    17161 tattatcctt ttagtaggat ttttagtaat cccttccctg ttaggagtta cactttgaac
    17221 tgtaccatct tcattgtact tgacatcata aaaacttggt atgtatactc cttctatact
    17281 agatatttta tataaaaaat cttctctagt cgttttattt ttcttccatt ctttatactc
    17341 atttactact tctaaattaa cttcttcacc ttcacctaat attactacat ctacaaaatc
    17401 tgctaagggt tcagaattat aagcacaagg accaccaacc attataaatg ggtcttccaa
    17461 agttctttct tcttttaata ttggtatatt ggctaaatct aatatattta atatattagt
    17521 atatgaaagt tcatattgaa gtgtaaaagt aacaaaatca aagtttgtta taggctctct
    17581 tgactctaat gcaaataatg gtatactttt ttctctcatt atattttcca tatcaactgc
    17641 aggagaatac actctctcac aaaatacatc ttcatcctta tttattacat catataatat
    17701 atgactccct aagtgactca taccaacttc atataaatct ggaaagcaat gtgcatatct
    17761 tatcaattca ctattactag tatctttatg aattgaattt atttcatttc caagatatct
    17821 agcaggcttt tctacttttt ttaagatttt tttcaagtct actttattca tttattaaaa
    17881 ctcctccaaa caagctcttt atttttattc ttatttataa gtaatattat acacgataaa
    17941 atattttcta tcaaaaatat aactaaaata ttgaatttaa aatacatatt attcacaaaa
    18001 tagaatagtt aaaaatattt aagaatattt tctattaaat ttttattgaa atacaaattt
    18061 tttacaaagt atgatatatt ataaacataa agctattgtt ttacattact tatcatgaca
    18121 tctcttgttt tattcacctt aaaatggggt atgttactta tataatgata aattttagga
    18181 gggttaaaaa tgagtaaagt ttttgaagta aaaaaagata tctactttac tggcgtagta
    18241 gatgaaggtt taaaagtatt cgacataata atggaaactg agtttgggac aacttacaac
    18301 tcttatttaa tcaaggacga aaaaacggtt ttatttgaca cggtaaaggc aaattttaaa
    18361 gatgagtttt taagtaattt atcagaagtt actgatatag ctaaaattga ctatgttgta
    18421 attcatcata cagaaccaga ccatgcaggt agtttaaaat acttattaga tataaatcct
    18481 aatatagaag tttattgtac taaagctgct aaactatact tagatggtca aataaataga
    18541 ccttttaact gtcatgtaat aaaagatggt gaaattttaa atattggtaa aagaaatctt
    18601 agatttataa ctgctccatt cttacattgg gttgatacaa tgtttactta cattgaggag
    18661 gacaagactc ttttaacttg tgatgcattt ggttgtcact ttgcaagtgt tgatgcagat
    18721 gtagtaaata gcgaagatta cctaaaatca gctaaacact actatgattg tatagttaag
    18781 ccatttgcta aacatgtttt aagtgctgta gataaggtgg taggactaaa catagaattt
    18841 gatactatat tgacttctca tggtccaatg cttactaaag acccaatggc tgctgttaaa
    18901 agatatgttg agtggtctac agaagcggtt aatacaacta atcaaaatca agtttctata
    18961 ttctacttat cagcttatag caatacttta gagatggcta aaaagataaa agaagggctt
    19021 gataaagaag gtgcaaaggc tgaattatat gatttagaag atatgacatt aacagaaatg
    19081 cacgatacac tagtagtatc aaaagtaata ttattaggtt ctccaactat aaacaaaact
    19141 atggttaaac ctatgtggga tttattctct gttatagacc caatggctaa ccaaggtaag
    19201 atagcaggag tatttggttc atttggttgg agtggtgaag gtataactat ggctgagact
    19261 ttacttaaat ctatgtcatt caaaatgcct gtagaatctc ttaagaaaaa attcttccct
    19321 agtgaagaga cattaaaaga gtgtatggca tttggtgcag aatttgcaaa attagttaaa
    19381 tagccattaa tcacacatac tattatttaa aataaatgat tttatattta aaaataaatt
    19441 aaaatggagt tgtctcaaaa ttaatttttc taatctaatt ttaagttcaa ctcctttttt
    19501 attttccaaa atgactaata taatgataaa tgcaccctca ccaagagggt gcattgttta
    19561 taatctgttc tatttgtata ctagtataat tgtattaatt cacgtatcta gcatatccta
    19621 cttgccctat tccagaatta gatgttgttt caccaacttc tactttaata attacattat
    19681 tttctttaca cttttttaat aattctgttg tagaaaggtt cttagcagta aaatctaaag
    19741 aaaaggggtt atcatattca aaaggataac taacaggttc accatctgga gctaatgcaa
    19801 ccataccata tggtatattt ataaacccta ttacagaaat tgtatgcttt gatgggtcta
    19861 aatttgaatt tttgcttata aactccctct gctgcgttcc agttaacata tgttgtgttg
    19921 tttttaatgt gattgtaagc aactctaatc ttagcatctg tggtcatgtt ctttgtaact
    19981 ttcttattca taaagtctgc ttttgcttgt gatactgaat taagttgagc ttataacaac
    20041 tttggagcat atatactgtt ggtttcagct acatagcttt tcggtgtctt tgcagtattg
    20101 tcaataatac atttaaaatt aaagttgtat acacttcata cattctcccc taacaacatt
    20161 atacagatta taatttgtat attcagtaca ttttttgaaa agtataatta tatcaaaata
    20221 tgtgattatg tatagataca atccactatg gaaaaatata taataaaaga gtataacaaa
    20281 caataaaaaa taagatgatt caaattttat tttgaaacat cttatttact ctacaaatta
    20341 aaattacttg gtgaattatt taattattat tttttattaa agaaatttta aatcttaata
    20401 taaaatatgc tattatacat aataatccaa gtgcaaatgc tactggaatt aacatctccg
    20461 attcaaagta aaagaaatat tctaagaaaa tatctggatt tactatattc ccattcttgt
    20521 ctaaaaaatc tttcccatta tatcctaagt gctgtataaa attacttatg ttattataat
    20581 ctgcttccct atcatacacc atattagcat tcaatccaga agaacttaaa ttagtgtttc
    20641 catctttcat atctattaca tataatctaa gagctttgac tttactatac ttatctttat
    20701 caagtatttt ttcaagttct aacttatatt catcaatctt tgtcatatca tgcctttctg
    20761 ctaattgctt tatttctcca tcaagttctt ttcctaaatt atacatatta tgttgtaaat
    20821 tattattgta ataccacata aatccagaaa ttaaaaatgc aattatactt atgtaaccta
    20881 atactttttt aaaccacatt acaatggatt tatgcctctc caatgataaa gatagttctt
    20941 ttattatatt acataattca tactgcattt catcaccatc atcaaatctt tcaattgctt
    21001 ttttacatgc ttcttcttca tctaatcctt ctaatttaaa ttcatttgca gattctataa
    21061 gatggcatct catttcctgt tttaattcta tagtactttt attgtcacat tttttataca
    21121 gtttatctaa atattcatct attagtttca tgaattaatc cacctctccc aaaaattgat
    21181 tcataacctt tttaataaat ctccattctt gtttctttat ttctagaaaa tctttacctt
    21241 cattagttaa attataatat tttcttcttc cacctgaact ttgttcattg ctataataag
    21301 atttaattaa atttttagat tccattcttt taagtgcaag atacattgtt ccttctttta
    21361 attcaaactc acttctttct cttacttgct ttgcaatttc atatccataa caatctttct
    21421 tttctaatat agaaacaatt aatatatcga tataaccttt taacacttct ttatttaact
    21481 gcattatgta ccaccgcctt ttcacctact actctataat gtatagtaat tatatttact
    21541 ataataatac atagtactat ataatacaag gtttatattc tataatagac ttaaaaataa
    21601 taaatagctg tatattaata tttaat
//
LOCUS       DAMJVM010000059        20942 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.062, whole genome shotgun sequence.
ACCESSION   DAMJVM010000059 DAMJVM010000000
VERSION     DAMJVM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.062"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(159..1001)
                     /locus_tag="PC523_002351"
     CDS             complement(159..1001)
                     /locus_tag="PC523_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2475865.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKNK
                     IERTFIIQDESFLDKIGKLASQVDEKFFAISTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENIIIKNELLHEIKRIHKKEYEIGKWAVDYINKEFGVKFPLDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     REKINDTSNEIIDIIKVKYEKDYNCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(1261..3303)
                     /locus_tag="PC523_002352"
     CDS             complement(1261..3303)
                     /locus_tag="PC523_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDF2475866.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWNNIPLAIYQIVLGIILSILP
                     FKFLFSFNPEIFVICIIAPLLFSEAQNVSRKELLELRKPILLLAFGLVLTTVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFIITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFIYFVAEEVGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNEDMTLKLTFISALIVFILFVIRFIWVYLWH
                     DSFIKTKKNPLNNFFVGFLGFKDEEAVKENISRCKYSLIVATCGVHGTFTLATALSIP
                     FHLANKDVFPMRDTVLFISSEVILISLILATVLLPRLLKSNSQESEHLLSDKEAYKLI
                     LKEAILKLSNEKAQEAQLVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADKQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIIKLKISMWTIDRKVKKEVKEKIT
                     KIVSENKEIIKEFHYAYALSTKTAISFINKNTDENNRHEALISIRFYNRYLNWITRKI
                     QNDDKFESRVEYYRVMAIQSQRDSVQQLVENKQISREVAKELLENILYDEMMMIES"
     gene            complement(3454..4425)
                     /locus_tag="PC523_002353"
     CDS             complement(3454..4425)
                     /locus_tag="PC523_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDF2475867.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFGKEIKFVKAVDGISFDLKKG
                     ETLAIVGESGCGKSSAGRTILRLIEKTEGKVEFNGIDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHDLVPKNEIKDNILNIVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKEELYLHSQHPYTKALMSAVPVQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEVPVLKEISRNHFVACHLI"
     gene            complement(4426..5403)
                     /locus_tag="PC523_002354"
     CDS             complement(4426..5403)
                     /locus_tag="PC523_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2475868.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNGVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFQGKDILKMNENELMRIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMLHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVVVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPVLGKNKDKRLYSIPGQV
                     PNPIGIPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            5622..7181
                     /locus_tag="PC523_002355"
     CDS             5622..7181
                     /locus_tag="PC523_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2475869.1"
                     /translation="MKFKKLASLILVSALMLTFTACASNDKEKSNGGKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNED
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQNTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAKIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIEDAKPSPAI
                     LFEDWSKLYVE"
     gene            7287..8246
                     /locus_tag="PC523_002356"
     CDS             7287..8246
                     /locus_tag="PC523_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2475870.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     AQDIENIRVNMGLDKPVYVRYLSWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKISSIFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVVTAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            8249..9061
                     /locus_tag="PC523_002357"
     CDS             8249..9061
                     /locus_tag="PC523_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2475871.1"
                     /translation="MFTNILKELKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKISTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQKYIFDKLYLAVFPGLLIFLTVMSFTIISKKFSSNT"
     gene            complement(9276..10178)
                     /locus_tag="PC523_002358"
     CDS             complement(9276..10178)
                     /locus_tag="PC523_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2475872.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKIRIGIPPL
                     ILGIVFSDVVAQLVANNPDIEFDIVERGAYDLSRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNYPITWKVVLCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            10531..11709
                     /locus_tag="PC523_002359"
     CDS             10531..11709
                     /locus_tag="PC523_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDF2475873.1"
                     /translation="MKDVVIVSAVRTPIGNFGGVFKNTSAVQLGTITVKESISRIGLN
                     LSEINEVIIGNVLQAGLGQNVARQIAINAGIPNSVPSYTINKLCGSGLKSVQLAAQSI
                     ASGENDVVIAGGTENMSQAPYIVPTARFGNKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAEKAIKNKRFKEEIIPVNILIRRGKMETIDTDE
                     YPKLGMTFEGLSKLKPAFKKDGTVTAGNASGINDGAAMLILMSKQKADELGIKPLVKI
                     KSYASAGVEPEIMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVKTGLATLCIGGGQGISMVVSR
                     "
     gene            11718..12374
                     /locus_tag="PC523_002360"
     CDS             11718..12374
                     /locus_tag="PC523_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HDF2475874.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKTTIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            12374..13039
                     /locus_tag="PC523_002361"
     CDS             12374..13039
                     /locus_tag="PC523_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HDF2475875.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDDVNV
                     ILQSENGFLGLGAAEDGNSNDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISSTEAPLILSDSLNGNI
                     SVV"
     gene            13144..13920
                     /locus_tag="PC523_002362"
     CDS             13144..13920
                     /locus_tag="PC523_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HDF2475876.1"
                     /translation="MLKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNKT
                     TTELQQEGYDCMNVKCDVTKEEEINQAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            13982..15316
                     /locus_tag="PC523_002363"
     CDS             13982..15316
                     /locus_tag="PC523_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2475877.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVLLNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSVAGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLARELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            15817..16770
                     /locus_tag="PC523_002364"
     CDS             15817..16770
                     /locus_tag="PC523_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HDF2475878.1"
                     /translation="MRKNIPTKLKTILALGVLTLTTASSAVNASALSLENNVNTSNKN
                     LVYSNTRYKFSAKPNCSIKPELKPDKDNSCKDKNHSDKNCNNTNKPDNNKPESNKPDD
                     NNNSGSTDKPDNNKPESNKPDDNNNSGSTDKPDNNKPESNKPDDNNNSDSTDKPDNNK
                     PESNKPNDNNNSDSTDKPNDNNNSGSTSENFSAYQKEVVDLVNIERSKAGLNPLTLDS
                     SISNVATKKSQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVV
                     NAWMNSEGHRKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(17174..20713)
                     /gene="nifJ"
                     /locus_tag="PC523_002365"
     CDS             complement(17174..20713)
                     /gene="nifJ"
                     /locus_tag="PC523_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDF2475879.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLVAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     SIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGVTMSHLRFGDSPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHACIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRIQV
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKVDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVLEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         7244 a         3276 c         2940 g         7482 t
ORIGIN      
        1 taaactatta taattttttt ataaacataa gtcttgcctg cattatcagt aacaatctta
       61 atgttttaaa attattgttt tatattaaat tttaaataat atattaaaat taaacataat
      121 atactaaacc tatacatttt gtattaaact actctttatt ataaattcaa tacagatata
      181 actcttttga tatgtaatgt taaataaagt agttcatctt gacttacata gtaatcataa
      241 ttcttttcta catgagtttt tattttgtat gcacaattat aatccttttc atactttact
      301 ttgattatgt ctataatttc attacttgta tcattaattt tttctctttt tactaaccgt
      361 tttgcaaaga attttaaatg agtcagaagc ctatcataat ttatatcatc ttctttaaat
      421 tcaactctat aataatatct tataatattt agtatgtctt ttacaatctt agtaattaac
      481 aatgattcct ttgaacttcc tttatagttt gaatttacta tatgcattgc tataaaacca
      541 gcttcatcca atggaaactt taccccaaat tctttattta tgtaatctac agcccacttc
      601 ccaatctcat attctttttt atgtattctt tttatctcat gtagtaactc attctttata
      661 attatatttt catgatatct tttaattgca aatgcaatat ggtcagtaag tgccacataa
      721 atatattcat aaagcttagt attcaaattc tcttctgcat aagcaattat ttcagtacta
      781 atcgcaaaaa atttctcatc aacctggctt gctagtttac caattttatc taaaaagctt
      841 tcatcttgta ttataaaagt tctttcaatt ttatttttat ctatttcttg acctttcttt
      901 ttttgaaacc caatcccaca tcctgttagt attaattctt tttttatatt ttcatctaaa
      961 actaatacga cattattatt ataaatttgt tgaataatca taatcaatat ctctcccaaa
     1021 atacaatttc ccttattcat aaaaaaatac ctaaattaag atacaattca cctgtatacc
     1081 ttaacctagg tattgcctgc ttaccagtaa caatccagcc tgttaacgct tatatattgt
     1141 attcaattta tggttttatt ctattataaa taaatatata tgtcaagtaa attattgaaa
     1201 actatttcct attttttgta ttaattctaa catcaaaaat caaattttat tccattatat
     1261 ttatgattct atcatcatca tttcatcata tagaatattt tccaataact ctttagccac
     1321 ttctcttgat atttgcttat tttcaactaa ttgttggaca ctgtctcttt gactttgtat
     1381 tgccattact ctgtagtatt ctactcttga ctcaaactta tcatcatttt gaatctttct
     1441 agtaatccaa ttcaaataac gattatagaa tctgattgat attaaggcct catgtctgtt
     1501 attttcatca gtatttttat ttataaatga tatagccgtt ttagtagaaa gtgcatatgc
     1561 atagtgaaat tctttgatta tttctttatt ctcagacact atttttgtaa ttttttcttt
     1621 tacttctttc tttacttttc tgtctatagt ccacatactt atttttaatt ttataatttt
     1681 aaatatagat ttttgaacag attttcttga ttttgtaata taaaatctat acaatttaaa
     1741 tgctctatca gaaattttac catcttctaa tagtttcaaa acagccaata atccctgttt
     1801 gtctgcttct gcaatcatca tacttataag tttattatct ggagtattta atattccttt
     1861 ttctgagtct actaactgtt catttaaatc atgtataact aattgtgctt cttgtgcttt
     1921 ttcattacta agttttaaaa tggcttcttt caaaattagt ttatatgcct ctttatcaga
     1981 taataaatgt tctgactctt gtgaattact ttttaataat cttggcagta aaactgttgc
     2041 caatattaaa cttataagaa taacttctga tgatataaat aaaacagtat ctctcattgg
     2101 aaatacatct ttattagcca aatgaaatgg tatagataag gcagtagcga gtgtaaacgt
     2161 accatgaaca ccacaagtag caactattag tgagtattta cacctagaaa tattctcttt
     2221 tacagcttct tcatccttaa aacctaaaaa acctacaaaa aaattattta aaggattctt
     2281 tttagttttt ataaaagaat catgccataa atatacccat ataaatctta taacaaataa
     2341 aataaataca attaaagcag aaataaaggt caatttcaat gtcatatctt catttttact
     2401 actaagtcct gtatatatac ttggtaacag aaaaccaagc agtgtaaaga caaacccatt
     2461 caatacatac tccaaaacat accaagtgtt gtctgatatt atccttaatt tagtagttgt
     2521 attttgtaaa tgctcttttt caattccatg agctatccct gccatcacaa cagctaggat
     2581 tccagatact cctacttcct ctgctacaaa atatataaaa agtggagtca ttatttgtat
     2641 aactattacc attggaattt cttccaaatt ccacttatgt aggctaagtc ttatttttat
     2701 aattatgtat cctaaaatac ttcctactat tattcctcca aaagctgtaa ttataaattg
     2761 aatccctgcc tcttctattg aaaaaacgcc tgtaactgtg gcaagtactg caaccttaaa
     2821 tgctactact ccagcagcat catttaataa agattctcct tcaagtatac tcatcatatt
     2881 tttaggaaaa tttaatcctt gtgtgattga ttttactgct actaaatcag ttggagatat
     2941 tactgctgcc aaagcaaatg aaacagataa tggcatccca ggaataagaa aatgtataaa
     3001 aattccacct gcaaacacag ttgttaaaac taaaccaaat gcaagaagca aaattggttt
     3061 tctaagttct aacagctctt ttcttgatac attttgagct tcactaaata gtaatggagc
     3121 tataatacat atcacaaata tttctggatt aaatgaaaat aagaatttaa atggcaaaat
     3181 agataatata atccctaata caatttgata tattgcaaga ggaatattat tccacttttt
     3241 gctaaataca gttgaaatcg ttgtacaaac caataatccc aacaatgtta ctaaaattga
     3301 cataactacc tctcttttca ataataatat tatagattaa acttctgtat caactaactt
     3361 aatattaaca acacgaaatt ccatcttcat caaaaaattt tcaatttatt ctattattct
     3421 tattatatac attaaattta atttttgaaa tatctatatt agatgacatg caacaaaatg
     3481 atttcttgat atttctttta aaactggaac ctcttttttg catatttcct tacaataagg
     3541 gcatcttgta tgaaacttac atccacttgg tggatttgtt ggagatggta tgtctccttt
     3601 taatataatt ctatttttct taattgtagg gtcttgtacg ggtactgcac tcataagtgc
     3661 ttttgtatat ggatgttgag aatgtaaata aagttcttct ttggttgcaa tttctattat
     3721 agaacctagg tacataacac aaactctatg tgcaatatga tttactacac tcaaatcatg
     3781 agatataaat aaatatgaga gtttatattt gtcctgtaaa tccataagta aatttataat
     3841 ttgagattga atagaaacat ctagtgcgga aacaggttca tcacaaacta taaattctgg
     3901 atttaatgct aatgctcttg ctatacctat tctctgtctt tgacctccag aaaactcacc
     3961 tggttttcta tttaaatggt acttagaaag accacaactt tctacaatat ttaatatatt
     4021 atcttttatt tcattctttg gcactaaatc atgttctata actgcctcag atattatttg
     4081 ctccacattt aatcttggat ttaatgaact atatgggtct tgaaatatca tttgcatttt
     4141 gggtctcaac tttctaagtt cttccttctc taacttatat atatctatac cattaaattc
     4201 aactttacct tcagtttttt caattaacct aagaatggtt cttcctgcac ttgattttcc
     4261 acatcctgat tctccaacta tagccaaagt ttctcctttt tttaaatcaa aagaaattcc
     4321 atctactgcc tttacgaatt taatttcttt gccaaataat ccagatttaa cttcaaagta
     4381 ctttttaaga ccttctactt ttaataaact ctcttctttt ttcaattata tctcctcctt
     4441 tttatataac caacatgcaa tactatgacc actattcaat tctattaaag gaggtatctg
     4501 agttctacat ttatcacata ctttttcaca tctatcacta aaataacatg aatctggtat
     4561 tccaattgga tttggtactt gtcctggaat tgaatataat cttttatctt tgttttttcc
     4621 aagaacaggc tttgatttta gaaggccaat agtataagga tgctttggat ttttaaacaa
     4681 ttcaacaacc tctgctactt ctataatttt gccagcatac atcactacta ctttatcaca
     4741 catttctgca actactccca aatcatgtgt aatcatcatt atagatgtat ttaatttttc
     4801 ttttatattt ttcattatat ctagaatctg agcttgaata gtaacatcta aagctgttgt
     4861 tggttcatca gctataagta attttggatt acatgataaa gccatagcta tcattattcg
     4921 ttgtctcata ccacctgata attcatgtgg atatgaatta tatatttctt cagctcttgg
     4981 tatttctacc agttttatca tatctattac ctttttctta gcttcttcct ttgataaatc
     5041 ttgatgtaac ataatacctt caatgatttg acttccaaca gtaaatgctg gatttaaaga
     5101 tgtcattggt tcttgaaaaa tcattgatat ctcattccct cttattctca taagctcatt
     5161 ttcattcatc tttaaaatgt ctttgccttg aaagattatt tctccacctt ctatttttga
     5221 tgttggaagt aacttcatca aagacatggc tgttacagat ttaccacatc ctgactctcc
     5281 aactattcca atagtttcac cttcatttat attaaaactt actccattta cactctttac
     5341 aattccttta tctgtatgaa aataaacttt taaatcttta acttcaatta gtgctttact
     5401 cattattttc tcctctcagc aaaattgctt aaaataacat acaccttttg attgtatttt
     5461 ataaaaattt aaattacaac attttattac attttattat ttttttctat taattataat
     5521 attgtaatag ttaatttcta tatatattga tttaataatg attattatta tacaatgaaa
     5581 aatatgtgtt aaatattatt ttatggaggt tttataagaa tatgaaattt aaaaaattgg
     5641 catcattaat cttagtttca gcattaatgc ttacatttac agcttgtgct tctaatgata
     5701 aagaaaagtc taatggaggc aaatctggtg gaactgttgt aatccctatg gcaagtgacc
     5761 ctgatataat caatggagct tttgcaaatg ttaaagaaga ttctcttgca tcaaatatag
     5821 tttatgctcc tctttatact tatgaaaaag ggaatttagt aaattatcta gcagaaaaag
     5881 tagattttaa agatagtaag gaattaacta taaaattaaa atccaactta aaatggcatg
     5941 atggaaaacc aataactgct gaagatgttt tatttacatt taatacagtt ttagacgaaa
     6001 aacaaaattc accatcaaga caatacttat tggtgggtga gaaaccagtt aaagtagaaa
     6061 aaattgatga tttaacagtt aaaattactt tgccaactgc aagtgaatct ttcttatatg
     6121 gtatatctaa aatttctcct ataccaaaac atgtgtttga aggagaatca aatatagcta
     6181 agtcagaaaa aaataataat ccagttggtt ctggtgcatt caaatttaaa gaatggaaaa
     6241 aaggtgaaag catagtattt gaaaagaatg aagactattt tggtggagaa cctaaggcag
     6301 attctatagc actaaaaata atacctaatg aagcatctca agaagcagct ttaaataatg
     6361 gtgaaatttc acttatgaag acatctgcag aaggatatga aaaggcaaaa tcaaactcaa
     6421 acttacaaac ttatacttat agtgaagaaa gattaaacta cattgtattt aaccaaaata
     6481 tatcaaacat ggctaataaa gaagttagac aagccttatc atatgcatta aatagaaatg
     6541 aaatgataga atcagcgtat ggaaaagaag gctctgtacc tgctaaatct atattagttc
     6601 cagaagcaga cttctataca gaagaaggtg ttgaaggata tgaccaaaat acaaataaag
     6661 ctaaagactt attggataag agtggtgtta aaattgataa attaaaaata gggtataaca
     6721 caggtagatt tgggcataaa aactatgctt tagtagctca acaagagttg aaaaaaattg
     6781 gtatagaagc tgaaatagtt ccttatgaaa gtaaggcatt cttcaatata ctatttagta
     6841 atagcactga atgtgatatg tatgtaaatg gttatgcttg gggattagaa ccaaatccat
     6901 atagaggtat gtttgaaact ggtcaatatt gtaaccaaac taaatattct aatgctaaaa
     6961 tagatgcttt atgggaaaaa ggatttactg agttaaacaa ggaaaaacgt gaagaaatat
     7021 ataagcaaat acaacaggat atatctaagg atgcacctat ttacactata gattatgaac
     7081 aaaatttaat ggcagctcaa aaaaatctaa aaggaataga agatgctaaa ccatctcctg
     7141 caatattatt tgaagattgg tctaaattgt atgtagaata attgatgtaa aattttatat
     7201 tattaattag aaaaggagat gtctaaaaat aatattgttt tattatttta aaatgagaca
     7261 tctcttttaa ttaggagaat ttacttatgt taaaattaat attgaataga gtgaaagttt
     7321 taatacctat gttaatctta atatctatat tatcatttgg acttttagaa ttagctcctg
     7381 gtgacccagc agatgcatat ataaatcctt taatgtctgc acaggatatt gaaaatataa
     7441 gagttaatat gggactcgat aaaccagtat acgtaagata tttaagctgg ttgaaaaaca
     7501 ctttaagcgg aaatttaggt atttcttata taaaccatat gcccgtaaca gaacaaatta
     7561 tggaaaaaat gggaaataca tttattctta tgggtacttc tttaatattt tcaatattgg
     7621 tagctatacc tcttggaata tttttagctg taaataaaaa ttcaataact agtaaaatat
     7681 catctatttt taattatata ggtgtttcta tcccttcttt ttggataggt atgatactca
     7741 tttcaatttt ttcagttaaa cttaacattt ttccatcagg aggtatgcat actattggaa
     7801 atgatagtat tggagattta gtaaaacatc tagttttgcc tgtaataact ttaggtctat
     7861 ataatactgc tatttttaca aattatgtgg aagctagtgt aaatgaacaa ttaaaaaaac
     7921 agtatgttgt tacagcaaga gcaaaaggat tatcagaaaa agtcatacta tttaaacatg
     7981 tcttgaaaaa ttcactgaca tcattggtta caatcctagg catgagtata caaaaattag
     8041 ttactggcgc atttgttaca gaagtagttt tctcttggcc aggtatggga agattaatga
     8101 ttgattcaat tttttcaaga gattatactg taattatggc aataactatg ttatcagcac
     8161 tatttttaat tttaggtaat ttggttgctg atatactata cttattgatt gaccctaaaa
     8221 taaaatctag caagggagga ttctagttat gtttacaaat atcttaaaag aattaaaaaa
     8281 agacaaatcc tttgcttttg catgtattac tcttagtata ataatcatat taattatagc
     8341 ttcttttata ttaaatatag actctaatgc aattgatact acttctaaac ttcaagaacc
     8401 tagcttaaag catatctttg gtacagatga acttgggaga gattacttct ctagagctct
     8461 ttatggtgga agaatttcac ttagtgttgg tattttatca atgcttattt ctgtagtatt
     8521 tggtactgct gtaggtgtta ttagtggata tattgggggt aagctagacc aattcttgat
     8581 gagactagta gatatgctta tgagtatacc aagcttctta gttttgattg tattaaatac
     8641 ttatctaaca cctaaaattt ccactattat actaattatt ggattcttaa gctggatgga
     8701 agtttcaaga attgtacgtg gcgaaacatt aaaaataaag gaaaatgaat actgtctagc
     8761 tgctaaagct accggtatca agactagaaa tatactatta agacatatag ttttaaactt
     8821 aaaagagact attgtagttg ctggctcatt aaatattgct aatgctatct taactgaatc
     8881 tgctcttagt tttttaggtc ttggaatcca attgcctctt gcatcatggg gtaatatgct
     8941 tcaagatgct caaaaatata tttttgacaa gttatattta gctgtatttc caggactact
     9001 tatattttta acagttatga gttttactat aataagcaaa aaattttcta gtaatactta
     9061 ataaatttta tatagaaaat aaaaacagtg attatccaaa gatatacact gtttttattt
     9121 ttcgttttaa aaagcacata ttttgtataa tataacttta aatactactc tagcatttat
     9181 gtaaaacctg ctattgctct caagcacgta gatgaaaaac taatagttta ttataataaa
     9241 aatggtttta tttatttgtt attttaataa cacttttaag agttcttttt actaaagaaa
     9301 tctataataa atttaaatac atgttggtct acatggctat aacgttcttt tttaggtcta
     9361 catagtacaa ctttccatgt aataggataa ttgaaaggtt tttctataat tttgctattt
     9421 tcaaaaaaat catgaactgg tgatggtaga atggttataa aatcagaatt tgtagtcgat
     9481 agcaacaaaa aatcccatga accagacatt atatatctct ttagtttaac attttcatca
     9541 gtaaattttt gcattagctt atgatgtatc ataaaagtag gattaaatat tgcaagtaac
     9601 tgctcattca aatcgttcca atctatctta tccttcttag caagtggatg atttatattt
     9661 aaaaatgctg taagttcatc ttcttgaagc acatgttcag tcactacact tttatcaatt
     9721 ttatgtggat gtaatagcac tgcataatct aattcttgta agataagcat tctacttaaa
     9781 tcatatgctc ccctctctac tatgtcaaat tctatatctg gattatttgc aactaactgt
     9841 gcaactacat ctgaaaatac tattccaagt ataagtggag gtattcctat tctaattttt
     9901 cctttaaatt gaactgaatc ttctcttaaa tcttccatca tatttttgta ttcatttata
     9961 atatgttctg catttacata aaatctttcc cctgctggtg ttaacccatt taacctacct
    10021 ttataccttt caaataaata tacatcttca ctttcttcaa aattttttat aatttgactt
    10081 agtgctggtt gagatatatg aagtgtctta gatgctattg ataaattaaa accagatttc
    10141 actatttcaa caaaatattt taactgtttt atatccattt taacccccct tccccaaaag
    10201 tataatttag attatatttt ttcttataat gatattttat aggaaaatca acataatttc
    10261 attaatattt taacaaaata tttttctaaa tctgctctaa atttttgtcc tataaatttt
    10321 tcttatacac tatataaaaa attagactta gacaaaacgc ttttttcgac gtaatctata
    10381 attagaaaca ggaaaaccta aggcattggg aataaaaata attgaaatac ttaaaaacaa
    10441 gcttgtatta gaagtacttt attatagtgt tccatatttt tcttattcag aaaattttta
    10501 attatcattc aagaaaggta actattagat atgaaagacg tagtaatcgt atcagcagtc
    10561 agaacaccca ttggaaattt tggaggtgta tttaaaaata cttcagctgt tcaacttgga
    10621 actatcactg ttaaggaatc aatttcacgt ataggactta atttatctga aataaatgaa
    10681 gtaataattg gaaatgtact tcaagcagga cttggacaga atgttgctag acaaattgct
    10741 ataaatgcag gtatcccaaa ttctgtccca agttatacaa taaataaatt atgtggttca
    10801 gggcttaaaa gtgttcagct agctgctcaa tcaattgcat ctggtgaaaa tgatgtagtc
    10861 attgcaggtg gcactgaaaa tatgagccaa gctccttata ttgttccaac tgcccgtttt
    10921 ggtaataaaa tgggcgacat aaccatggta gacagtatgc taacagatgg tcttattgat
    10981 gctttcaatc aatatcacat gggaataaca gcagaaaata tcgcaactaa atttgaattt
    11041 actagggaaa tgcaagataa attagcatta gaaagtcaaa ataaggctga gaaggctata
    11101 aaaaacaagc gttttaaaga ggaaatcatt ccagtgaata tcttaatacg tcgtggaaaa
    11161 atggaaacta ttgacacaga tgaatatcct aaattaggta tgacatttga aggtttgtct
    11221 aaacttaaac ctgcattcaa aaaagatggt actgtaacag ctggaaatgc atctggtatt
    11281 aatgatggtg cagccatgct aatactaatg agcaaacaaa aagcagacga actaggaatt
    11341 aaaccacttg ttaaaataaa atcttatgct tcagctggtg ttgaacctga aatcatgggt
    11401 acaggtccaa tccctgcaac tagaaaagca ctgaaaaaag ctgggcttag tatcaatgat
    11461 atagacttaa tagaagcaaa cgaagcattt gctgcccaag cattagctgt taaaaatgaa
    11521 cttcaaatag attcatcaaa gttaaatgtt aatggtggtg caatagcatt aggacatcca
    11581 ataggagcaa gtggagctag aatacttgtt accttaattt atgaaatgca aaaacgtaaa
    11641 gttaaaactg gacttgcaac cctttgtatt ggtggtggtc aaggaatatc aatggtggtt
    11701 tcacgatagg aggaaatatg aataaaatag ttagtattga tgaagctcta tctcatgtaa
    11761 aagatggaga tacaataatg gttggtggat tcatggccaa tggttcacca gaaaatttaa
    11821 ttgattattt atgtagtaaa aatataaaag atttgacact aatttgcaat gatactggct
    11881 ttattgacaa aggagttggc aaaatggttg taaataagca atttaaaaag ataattgctt
    11941 ctcatgtagg attaaataaa gaaactggaa gacaaatgaa caacaatgaa acagatgttg
    12001 agttagttcc tcagggaact cttgctgaac aaatacgtgc agctggttat ggtctaggag
    12061 gtatactcac accgactggt attggtactc ttgttgagga gggaaagcaa aaaattgttg
    12121 tcgatagcaa agaatacctt cttgaaaaac caatttatgc agatgttgct cttctatttg
    12181 catcaaaggt cgataaagca ggtaatctag tttataaagg ctctatgaac aattttaata
    12241 atttaatggc atctgctgcc aaaactacaa tagttgaagc agatgaaatc gttgaaattg
    12301 gcgatattga cccaaatgaa gttaatacac caggtatttt tgtaaattac atcgttgaag
    12361 gaggtaattt ataatggata aattagaaat gcaagaatat attgcaaata gagtatctaa
    12421 agaattaaaa gatggagctg ttgtaaacct tggaataggt ctgccaacca aagtagcaaa
    12481 ttatattcca gatgatgtaa acgttatctt gcaatctgaa aatggatttt taggtttagg
    12541 tgcagctgaa gatggaaatt caaatgatga aactattgta aatgcaggtg gtcaaccagt
    12601 tactatactg cctggaggat gtttctttga tagtgctact tcttttggaa taatcagagg
    12661 aggtcatgtt gatataactg ttcttggtgc acttcaggtt gacaaatatg gcaacatagc
    12721 aaattatatg attccaggta agatggttcc aggcatgggt ggagctatgg acttggtgac
    12781 tggagctaaa aaagttattg tagcaatgga acatacttca aaaggttctg caaaaatatt
    12841 aaataattgc actcttccac ttactgcaac aaatgctgta gatttgatag tcactgaaat
    12901 gggggttatg gaagtaacat cagatggaat tttacttaaa gaaataaatc cagctttcac
    12961 attggatgat gttatttctt ctactgaagc acctttgata ttgtcggatt cattaaatgg
    13021 aaacatatct gttgtttaaa ataattaaaa ctagaaaata aaaatgtctt agatgtatta
    13081 gatataaatt tatattactt aaaatattaa tataaaaata aaataaaatt ggaggaaatg
    13141 aaaatgctta aagataaagt tgtattcgta acaggagctg caagtggaat aggtaaacaa
    13201 atagctgaat cattcttaaa aaatggtgct aaagtaatgt tctctgatat aaatcaagaa
    13261 gctttaaata agaccacaac agagttacaa caagaaggtt atgattgcat gaatgtaaag
    13321 tgtgatgtaa ctaaggaaga agaaattaac caagctatag acaaaactgt tgaaaaatat
    13381 ggcagattag acatattaat aaacaatgct ggacttcaac acgtttctat gatagaagat
    13441 ttcccaacag ataaatttga atttatgata aaaataatgc taacagcacc ttttatagct
    13501 accaaaaaag catttcctat aatgaaaaaa caaggctttg gtcgtataat aaatatggca
    13561 tcaatcaatg gtgtaatagg atttgctgga aaggctgctt ataactcagc aaaacatgga
    13621 cttattggac taacaaaagt tgctgctctt gaagctgctg attctggtat aacagttaat
    13681 gctttgtgcc ctggatatgt agatactcca cttgtcagag gtcagtttaa tgacctttca
    13741 aaaactcgta atataccatt agaaaatgtc ttagaggagg ttttataccc acttgtgcca
    13801 caaaaacgcc tattagatgt tcaagaaatt gccgactatg ctatgttcct agcaagtgat
    13861 aatgctaagg gtattacagg acaagcttgc ttactagatg gaggatatac tgcacaatag
    13921 ttattatatt tagggcttga agaacttatt cttcaggtcc ttctactaag gaggaatatt
    13981 tatgagtatt atcgcaccaa ttggtataat tattggtatc atagctatta tatatttttc
    14041 agttaaagaa attcatataa ctatagctgc tccaatagca actttaatag ttgttctctt
    14101 aaataaaatg gatgttgtaa catcaatgct aggtgctgac aaaaacaatt atatgggggc
    14161 acttggaaac tatataatga gttattttgc aatattctta ttaggttcaa tacttgctaa
    14221 atttatggaa gaaagtggag ctactgtatc aatagctgat tttattttaa ataaatttgg
    14281 ttctaatcat ccttatagag ttttagttgc tatatttata gtaagtttta tattaacata
    14341 tggtggtata agcttatttg tagttatgtt tgcagttatt ccacttgcaa gaactctatt
    14401 taaaaagctt gatatttctt ggaaccttat tcagatacca ctttggcttg gtattgcaac
    14461 aattactatg actataattc ctggaacacc agccattcaa aatgttatac ctattcaata
    14521 tctaaataca tctcttactg cagctactat accaagtgtt gcaggtagta taggttgtgc
    14581 tacgtttggg cttgtctaca tgaaatatgc tctaaacaaa agcattagaa aaggtgaaac
    14641 ttatgcaact tacacttcag aattagaaga agaatcttta gctagggaat taccaggatt
    14701 ttttgcaagt atttttcctc tattagcact cgtaattata gctataactg gaagtacttt
    14761 tggttcagaa tttatgaaaa aaaatgtaat ttacatagct ttaatagttg caattctttt
    14821 atctgctttt ttattccata aatttttatc atctaagata cagacattga gtataggtgc
    14881 tagtggttct ataggtccta tatttgctac atcttctgca gttgcatttg gagcagtaat
    14941 aatggctgca cctggttttg atgtatttag caagcttata atgtcaatac caggtagtcc
    15001 acttataagt cttactgttt tgacttcaac aatgtcagca ataacagggt catcttctgg
    15061 tgcattagga atagtaatgc caaactttgc agattattat ttaagtactg gtctaaatcc
    15121 agaattaatt caccgtgttg ctacaatagc ctcaaatata ctaactatag ttccacaaag
    15181 tggtgttttt cttacattcc taagcctctc taagttaaca cataaaactg gatttaaaga
    15241 aactttcatt gtagttgcag ttggttcact aatagcagaa atcattgtaa ttacattagg
    15301 attattaatg tattaatata aaataaatca gaaattacaa tataaaaggg aatgtctcaa
    15361 aatgaatttt ttagttaatg agacattctt ttttgtatga aatcacatat acatttatta
    15421 tttcaacaat ggaagtgagg ctttctctat tttgatgtag acccttttat tccttaaaga
    15481 tttaaataat tataatttgt tactaatatt aaagcattta tttaataaat ttaactaaat
    15541 aaaaaaaaaa tttatcataa ctataaattt gcattataga attattataa aaatattatt
    15601 tgctctaaat aagtaattcc atactataca cacttctaat acaaactgtc aaacactctg
    15661 aaatatgcct aattattcca tatatgtctt ttttacatat ctttacagta aaaaaatact
    15721 tgaaattgtg ttagtataga ttcacgggta ccccctacac taaaggttac aaattagtaa
    15781 catttttaga atttttagat tgagaggtgt ttagtaatga gaaaaaatat accaacaaaa
    15841 ttaaaaacta tattagcttt aggagtgtta accttaacaa cagcttccag tgctgttaat
    15901 gcaagtgctt tatcacttga aaataatgta aatacaagta acaaaaattt agtatacagt
    15961 aatactaggt acaaattttc tgcaaaacca aattgttcta taaaaccaga acttaagcct
    16021 gataaagaca actcttgcaa agataagaat catagtgaca aaaattgcaa caatactaat
    16081 aaacctgata acaataaacc agagtctaat aaacctgatg acaataataa ttcaggttct
    16141 actgataaac ctgacaacaa taaaccagag tctaataagc ctgatgataa taataattca
    16201 ggttctactg ataaacctga caacaataaa ccagagtcta ataagcctga tgacaataat
    16261 aattcagact ctactgataa acctgataac aataaaccag agtctaataa acctaatgac
    16321 aataataatt cagactctac tgataagcct aatgataata acaattcagg ctctacaagt
    16381 gaaaattttt cagcttacca aaaagaagtt gtcgacttag tgaatataga aagatctaaa
    16441 gctggattaa atcctcttac tcttgattct tctatatcaa acgttgctac aaaaaaatct
    16501 caagatatga ttgataacaa ctatttttca cacaattctc caacttatgg ttctccattt
    16561 gatatgttga aaaaatttgg aataagctat aaaacagcag gtgaaaatat agctatgggt
    16621 caaaagacac caaaagaagt tgtaaatgcg tggatgaatt ctgaaggtca cagaaaaaat
    16681 ataatgaatc caaatttcag taaaataggt gttggagtag cacaaaaaag tggtggttca
    16741 atttattgga cacaaatctt tgtaggataa aatactcttt ttccatacat atcaaaaggg
    16801 tatctctaag tgataaaaaa cacacaaatg agatactctt ttgtatctat atttaaaatt
    16861 attaaataca aacatatata gtataaatag tattgttctc taccagtgat tttgatgtac
    16921 agaacaatat ataaaaagta ttggcaatat gaaaatggaa aacttaaaaa aggtatttag
    16981 cagataaaaa caaaatctta atatcaaaaa tgaaaaagag tcttgtctta aaatagagac
    17041 aagacccttt ttcattgttg atatgattaa aatatatttt attttatata aaaaagagtg
    17101 cctcatgaac taaaaagttc actttgagac actcttttta tcaattctat ataagcatta
    17161 taatacttta atattattca gctaattttt tgtatccttc atatctttct ttagcatcag
    17221 cttcagcttt agcaaataat ccatcagctt cctctgggaa ttgtttagct atagcagaat
    17281 atcttacttg tgcaagtatg aaatctttga agcttgcagt tggttctttt gaatctaaag
    17341 tgaatgggtt tttaccttca gcttttactt ctggattgaa tctatataaa tgccaatatc
    17401 cgcaagcaac agcttgtgct tcgttagcta cagttcttcc cataccttct tttaatccat
    17461 ggtttataca tggagcataa gctattatta gagatggtcc atcatgtttt tcagcttcta
    17521 gaacagcttt gataaattgg tttttatcag cacccatagc aacttgagct acatatacgt
    17581 ttccgtatgt catagccatc attcctaaat ctttctttct agatttttta cctgaagcag
    17641 caaacttagc catagcagca gctggagtag ctttagaagc ttgacctcct gtatttgaat
    17701 atacttctgt atcaaataca agcacattta catcttcacc agatgcaaga acatggtcta
    17761 atccaccata accgatgtca tatgcccaac cgtctccacc tagtatccat tgagatctct
    17821 taactagata atcttttcta tcttttatag cttttactaa ttctttagct ttttcatctg
    17881 tacaatcaca agctggtttt tctaataatt caacaacctt agcacttgct actttagaag
    17941 cttctccatc atttctagaa tctaaccact ctgtaaatac tgctttagca tcttcacaga
    18001 tatccataga aactagctct gtcatagcat ctactatttt gtttcttatt tgcttaacag
    18061 ctgtgtacat accaaatcca tattcagcat tatcctcgaa taaagagttt gcccaagatg
    18121 gacctttacc ttcatggtta caagtataag gaattgatgg tgcagaagct ccccatatag
    18181 aagaacatcc agtagcatta gctatcatca ttctatctcc atataattgt gtaactactt
    18241 taatgtatgc agtttctcca catccagcac atgctcctga gaactccatt aatggttgtc
    18301 taaattgact tcctttaaca gtatttgcag gcataacgtc aacttttgga gctacttcat
    18361 caggatttac tgcaaatgcc caattatcta actctgtatc aagttgagta tctattggct
    18421 tcataactaa agctttttct ttagctggac atatatcagc acagtttcca catcctgtac
    18481 agtcaagtgg actaacttga attctatatt ttaatccttc taatccttta cctaatgctt
    18541 ttttagcttc aaatccttct ggagcattag ctagttcttc ttcagtaact aatactggtc
    18601 ttatacatgc atgaggacat ataaaagaac attggttaca ttgaatacag ttatctatta
    18661 tccattcagg aacatcaaca gctataccac gtttttcata agctgcagta ccacaaggga
    18721 atgttccatc ttctatacca ttgaatgtac ttactggtaa gttgtttcct tcttgtgcag
    18781 tcattggtct tagtatattt tttataaatt ctggttcttt aacttctcct ttatctgcat
    18841 caacagcatt ttcccaagaa gctggaactt caactttaac taaagcattt attcctgcat
    18901 caacagcagc atagttcatg ttaactattt tttcaccttt tttaccataa gctttaacta
    18961 tagaatcttt taagtattta acagcatctt cttctggtat tatttcagct aatttaaaga
    19021 atgcagattg cattatcatg tttattctgt ttccaagacc tatttcttgt cctagtttaa
    19081 cagcgttaac tgtatagaat tttatatcat ttttagctat atattgcttc atatgagctg
    19141 gtaaatgttc ttcaagtcct gcttggtccc atatagtgtt aagtacaaat gtaccacctt
    19201 ttttaagacc ttttaataaa tcatattgat aaacatatga ttgtttatga caggcaatat
    19261 agtcagcttc atctattaaa taagtcgctc ttattggact atcaccaaat cttaagtgag
    19321 acatagttac tccaccagat tttttagagt cataatcaaa gtaagcttga gcgtattttt
    19381 cagtatggtc accgattatt tttatagctt gcttattagc tccaacagta ccatcagaac
    19441 ctaatcccca gaacttacat cttatagttc ctttagaagc tatttttatt ggttcagatg
    19501 gagttaaaga agtattagtt acatcatcaa ctatacttac tgtaaatcca tttttaggtt
    19561 gttctgaaac taagttatca aaaacagttt ttatatctga aggagtagtg tcttttgaac
    19621 ctaatccgta tcttccacct attatcatag gagcattttc ttttccatag aatacatcac
    19681 gtacatctaa gtataatggt tcaccagttg aacctggctc tttagttcta tcaagtacac
    19741 atattctttc aacagtagat ggcatagcat ctaagaaatg tttcattgag aatggtctat
    19801 ataaatgaac tttaactaaa ccatattttt ctcccttagc atttaagtaa tctatagttt
    19861 cttctattgt ttctgttaca gaacccatag ccactaatac gtatttagca tcttctgctc
    19921 cgtagtaatc aaataaacta tgttttctac ctgttaaatt actcattttt tccatgtatt
    19981 tttcaactat acctactata ttttgatagt atttattaga agcttctcta gtttggaagt
    20041 atatatctgg gttttgagca gtaccacgag caactggatg atttggagat aaagcattat
    20101 ctctaaatgc ttgaactgct tcaaagttta ataaacttgc ataatcttca ttttctaata
    20161 attcaacttt ttggatttca tgtgaagttc tgaatccatc aaagaaatgt ataaatggta
    20221 atctaccttc tattgcagct aaatgtgcaa caggtgctat atcagctact tcttgaactg
    20281 aaccagaagc taataataca catccagttt gtctagcagc cataacgtct tggtgatccc
    20341 caaatattga taaagcttga gatgctaaag cacgagcact tacatggaat actcctggta
    20401 ataactctcc tgcaacttta tacatgttag gtatcattaa taataaacct tgagatgctg
    20461 taaaagtaga agttaaagct cctccttgta aagaaccatg gaatgttcct gatgcacctg
    20521 cttctgactg catttcaaca acattaactg tttgtccaaa tatatttttt cttccttgtg
    20581 atgcccactc atcaactact tcagccatag ttgaagatgg tgtgattgga tatatagctg
    20641 ctacatctgt aaacgcatag gcaacatgag ctgcagctgt atttccatca agtgttttca
    20701 taaacttagc cataatatgt atcctccttg atagctacaa ttgtacttgt ttataacaag
    20761 tctatttttt tatatttaca tataatttac acaacaaaaa tataaacaca catatagtcg
    20821 cgcaaatact ctatcttcta atataatact acagattaga taaaattcct ttgaaaattg
    20881 taaattaatt acatatttaa gtagattttt ttttaaatta aatatgttaa gtttttttca
    20941 ta
//
LOCUS       DAMJVM010000060        20637 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.063, whole genome shotgun sequence.
ACCESSION   DAMJVM010000060 DAMJVM010000000
VERSION     DAMJVM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20637
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.063"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            <1..922
                     /locus_tag="PC523_002366"
     CDS             <1..922
                     /locus_tag="PC523_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2475880.1"
                     /translation="LLIAFTSIFLFLSISKPNNSELISSFSILFMYIPAFSAIVVLKK
                     VSNYKFDSTVDKFLKFFAITTIVRIVISIAETFLIYSSIISSTIDTLTSVYLLAVVLY
                     NEFQFEVLNLSLSKNFKKVLGILLIFLILIIVRNIIDKEGLSINVVNKFVTVFINIII
                     NLCFGFNLFFGEEFGWRYFLQPRLQKIYGKKFGVLILGFIWGIWHLPLCVTLYSPETP
                     FYCVISQISYCTFIGIFLGYTYMKTKNLWAPILIHLVNNTVSIILGGSIESIFTLESL
                     LWSILVNAILFLPFLFAKEYKANNYDESSN"
     gene            complement(1138..2013)
                     /locus_tag="PC523_002367"
     CDS             complement(1138..2013)
                     /locus_tag="PC523_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HDF2475881.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLD
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTVKKMSNYLYNDCFKIHYAVVDALIENRITE
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(2195..2881)
                     /locus_tag="PC523_002368"
     CDS             complement(2195..2881)
                     /locus_tag="PC523_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDF2475882.1"
                     /translation="MFGVIANSLAIAGGCIVGLVIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(2930..3469)
                     /locus_tag="PC523_002369"
     CDS             complement(2930..3469)
                     /locus_tag="PC523_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDF2475883.1"
                     /translation="MSDLNNKIIKERDENEASMNETKIYDILNKLNVEYDVVEHEAVY
                     TAEQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFA
                     SPERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVLKEDYLNFHPNINTKTVNISR
                     KGFEEFLEYLKYPIQSIDV"
     gene            complement(3515..4147)
                     /locus_tag="PC523_002370"
     CDS             complement(3515..4147)
                     /locus_tag="PC523_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDF2475884.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNKNIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(4234..5316)
                     /locus_tag="PC523_002371"
     CDS             complement(4234..5316)
                     /locus_tag="PC523_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDF2475885.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLKNDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLKFIKNIY"
     gene            complement(5449..6795)
                     /locus_tag="PC523_002372"
     CDS             complement(5449..6795)
                     /locus_tag="PC523_002372"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDF2475886.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGNGEKMIGILGHVDVVPEGDLESWNYPPF
                     EGIVEDGKLYGRGTQDDKGPTISAIYAVKVLMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDSEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTILVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKAAQIYALSIYELLKI"
     gene            complement(6864..8144)
                     /locus_tag="PC523_002373"
     CDS             complement(6864..8144)
                     /locus_tag="PC523_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HDF2475887.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEDIKGKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(8271..8528)
                     /gene="hfq"
                     /locus_tag="PC523_002374"
     CDS             complement(8271..8528)
                     /gene="hfq"
                     /locus_tag="PC523_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDF2475888.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNR"
     gene            complement(8642..9577)
                     /gene="miaA"
                     /locus_tag="PC523_002375"
     CDS             complement(8642..9577)
                     /gene="miaA"
                     /locus_tag="PC523_002375"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDF2475889.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEAEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIYEINEKGKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQNQLAEKGIDYMHNRLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSNDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMLKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLEQYENIDELKNEIILYIKDSIK"
     gene            complement(9598..11562)
                     /gene="mutL"
                     /locus_tag="PC523_002376"
     CDS             complement(9598..11562)
                     /gene="mutL"
                     /locus_tag="PC523_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDF2475890.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGMGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIRDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNDNLLESTPKNSNTTKSKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASEDEVNNINYLSEDSANDNIQEEFQVDGIRNEGNYYLGDSIKD
                     SEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKNDSMYLLDQHAAHERILYERYMEKF
                     YRQDINMQILLDPVIIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIFG
                     VPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLEK
                     CENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(11573..14416)
                     /gene="mutS"
                     /locus_tag="PC523_002377"
     CDS             complement(11573..14416)
                     /gene="mutS"
                     /locus_tag="PC523_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDF2475891.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSGMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAEYADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKVLNDEVALMKQNDDKEKTNQDDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(14579..15313)
                     /locus_tag="PC523_002378"
     CDS             complement(14579..15313)
                     /locus_tag="PC523_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2475892.1"
                     /translation="MLTQMNFYQNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLVL
                     KKEKRNKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRNLNEDNLENIIKLKKEIISLL"
     gene            complement(15282..16295)
                     /locus_tag="PC523_002379"
     CDS             complement(15282..16295)
                     /locus_tag="PC523_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2475893.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKNASSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVAAVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEEASKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPK"
     gene            complement(16317..17087)
                     /locus_tag="PC523_002380"
     CDS             complement(16317..17087)
                     /locus_tag="PC523_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2475894.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSVVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHSVVTIGEAVLGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MNSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQFVKKIVIRHRLTD"
     gene            complement(17062..17352)
                     /locus_tag="PC523_002381"
     CDS             complement(17062..17352)
                     /locus_tag="PC523_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HDF2475895.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(17484..17583)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(17708..17842)
                     /locus_tag="PC523_002382"
     CDS             complement(17708..17842)
                     /locus_tag="PC523_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2475896.1"
                     /translation="MNKNKNLAQCLDGIDIYPNMLEIVKQKLENYVNLVLGDTEKLSF
                     "
     gene            complement(17857..18030)
                     /locus_tag="PC523_002383"
     CDS             complement(17857..18030)
                     /locus_tag="PC523_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475897.1"
                     /translation="MIFLYYSGCEPQEEPCVVEINQESDYEITYLEYNFKEFIEGIFV
                     YKDECSIKTMLNQ"
     regulatory      18552..18641
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            18694..18870
                     /locus_tag="PC523_002384"
     CDS             18694..18870
                     /locus_tag="PC523_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475898.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(18985..19341)
                     /locus_tag="PC523_002385"
     CDS             complement(18985..19341)
                     /locus_tag="PC523_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2475899.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLESDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            19597..20517
                     /locus_tag="PC523_002386"
     CDS             19597..20517
                     /locus_tag="PC523_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HDF2475900.1"
                     /translation="MNKKFRLYQVDSFTKEKFTGNPAGVITNADGLSESQMQKIAREL
                     NNSETAFIFNKLAKNHQENYDVHIRFFTPTNEVPICGHATIAAHYVRAVENQLNNKTV
                     FQKTGAGILPVDIIKKDNDYEIIMTQGKIEVSPPLQQTYQERLLNAFGILKEDIRTDC
                     PIAISSTGHSKVMIGIKELSTLNSLSPNLYELSKISQEIKCNGYYIFTLHDKETLQVH
                     GRMFAPAIGISEDPVTGNANGPLGAYLVHYKVIPIPNTEFIFEAVQGEAIKRKGKMLV
                     KVIISDNKPVEVKIIGKAVIAFSTNIEIEL"
BASE COUNT         6641 a         3450 c         2215 g         8331 t
ORIGIN      
        1 tttactgatt gcttttacat caatattttt atttttgtca atatcaaaac caaataatag
       61 tgaacttata tcaagcttct ctatattatt tatgtatatt cctgcatttt cagcaattgt
      121 tgttctaaaa aaagtttcta attacaagtt tgattcaaca gtggataaat ttttaaagtt
      181 ttttgctata actacaattg tgagaatagt aatatctata gctgaaacat ttttaatcta
      241 tagtagtatt atttcttcaa caatagatac attaacatct gtttatcttt tagcagttgt
      301 tctttacaat gaatttcaat ttgaagtttt aaatttatct ttaagtaaaa atttcaaaaa
      361 agttttaggc attctattaa tattcttgat attaattata gttagaaata ttattgataa
      421 agaaggttta tcaattaatg ttgtaaataa atttgtcaca gtatttataa atataattat
      481 aaatttatgt tttggtttta atttattttt tggagaagaa tttggatgga gatactttct
      541 tcaacctaga ctacaaaaga tttatggcaa aaaatttggt gtattaattt taggttttat
      601 atggggaatt tggcatttgc ctctttgtgt cactttgtac agtcccgaaa ccccatttta
      661 ttgtgttatt tctcagatat catattgtac ctttatagga atatttttag gctataccta
      721 tatgaagacc aaaaaccttt gggcgccaat attaatccac ttagtaaata acactgtttc
      781 tattatactt ggaggaagta ttgagtctat atttacatta gaatcactat tatggagtat
      841 tctagttaat gctatattat ttttaccatt cttatttgct aaagagtaca aagctaataa
      901 ttatgatgaa agttctaact agtctacaaa tacatgattc tttagacata gaagcttttt
      961 atatttcata acaaaaccct cctagtttta gacagtttta cacattttaa ctgtctccta
     1021 agaggggtct agttcatttt gagaatggtt tttaatataa acgatttatt attaataagg
     1081 atattttttt atttaaattt atataatcga acatctaata tttaatgttt aataaaatta
     1141 tgcatttgca aaagtatata tggcattaac ccttgctaca tataaattct catcactaca
     1201 tccaatatat ctagcatatt tttcaatctt ttcctttgca ttttcaacat cattttcgtc
     1261 gctctccatt aactcattta tataaatatc cattaatgat gctttatatt ctcttatatc
     1321 ataacctaaa aattcagtca atctctcaat ttccttacta aaatctccat cttcttttac
     1381 caaaaattcg tatacatctt ccctatatgg atttaaagtt attatttgtt ttactacttt
     1441 acattcgtca gccttcccaa taaaaccatc tttataatta ttaaataagg cattagattt
     1501 ttttatagat tcttcatcaa tataagaatg tacctcagtt atacgatttt ctataagagc
     1561 atctacaaca gcataatgaa ttttaaaaca atcattgtat aaataattac tcattttctt
     1621 tactgtcgat tctatttcta aaaaactagc atgaccgttt tttttatttt tatttggtat
     1681 aataaaggtt ttatttaaat tattaaaaag tctcttaaaa tctaaatatt tttcacaata
     1741 aacactttta aaggtatcga tatctatagt tataattcca aaactcacta tagtctcaat
     1801 gcctttctta aaagactctt ctatatattt ttctcctaga cttaaacctc tatcagatat
     1861 ttgctttaga ttttggaaac tatctaagca atctctctca aagtttttag atatttctaa
     1921 agcatataat ttaaatattt tccttatctt attataatta ttttttgtat catcaaacct
     1981 gataatagct ccatttacat caaaagtaat cattgagttt taacctcccc attagtttca
     2041 tcaataaaat aaatttaata taaactttat aatcataata ttaaacttat ttatactata
     2101 attattaatt tgatgataaa agctattttc cttctaaata aaaaatttgt ctaaagtttc
     2161 taaaaaattt agacaaatct taggttatta ctttttaaat tattccaaat actccaaata
     2221 atattggtat aaatatagct ggtaacaaat tggctacttt tatattcgtc aatttaagta
     2281 catttagacc aagacctata attaataaac ttccagctgc tgtcatttct gtaactacag
     2341 gatcacttaa aaatccattt aaaaatgatg ctcccacaca tataatacct tcgtacaaaa
     2401 atacagatat tgcagaaaac attacaccta tacctaaaga agcagtaaat ataattgaag
     2461 ctattccatc tataacagat tttacaaata aagtatcatg acttcctgta agaccacttt
     2521 ctaatgagcc aactactgcc atagctccta tacagaataa taaactagat gatataaaac
     2581 cctctgctat agaatctttt ttatttcctt tagaaaattt actttgtaaa aaagcaccca
     2641 acttatttac ccatttatca atatctatta attctcctat caaagcacct acagctacgg
     2701 atattattgt cactaatgtg ttttttcctt ctagtgctcc tgatatacca atgtacaata
     2761 cacatagggc aattccattc attatagtat cgctcactct ttgaggtaat cctcctttta
     2821 ttacaagacc tactatacat ccacctgcaa ttgctagact atttgctatt actccaaaca
     2881 ttttttctcc cctcgattag ctaattgaaa tttatcctac tattaaaact taaacatcaa
     2941 tactttgtat tggatatttt aaatattcca aaaattcttc aaagcctttt ctagatatat
     3001 tcacagtttt agtatttata ttaggatgaa aatttaagta atcttcttta agtacatctt
     3061 tatctatgta aagctcaact tttctttctg tatcatttat taatgaaaaa ggatttacac
     3121 ttccaggaag aagtttaagt acattgtata gtctttcagg tgaagcaaat gataatctag
     3181 agctccctat cttatcttta agtgaattta aatcgacttg tttttctcct ttaactacaa
     3241 taagataata cttatttccc ttagcatttc ttaaaaataa atttttacat tggcatccct
     3301 ttgttattcc atctaaaatt actaattgct ctgctgtata tacagcttca tgttctacta
     3361 catcatattc aacatttaat ttattcaaaa tatcatatat tttagtttca ttcatgctcg
     3421 cttcgttttc atctcgttct ttaataatct tgttatttaa atcactcata ctttcttctc
     3481 cttaaaccct ccatcatcat atcattgtga gatattattc tactatagct attgctctta
     3541 ctggagctcc gtcagcatca ttatacttaa atggcgctgc tataaataga aatttttctg
     3601 gaacattttc taaatttgta agattttcta tgattatttt tcctttctca aataaaatat
     3661 gatgcacttc aaatactgat gtgtcatatc tatccactga taacatgtct attcctatcc
     3721 cttttatatt agtattagct atataatttg cagcttcttt tgttaatgta ggatagccta
     3781 catagtactg ctttttatcc caaaactttg accaacctga tttaaatatt ataaaatcac
     3841 tattttttat ttcatcttca tattttttta gatattctaa ctctatattc ttatttccct
     3901 tttcaagctc caatacataa gctttcccaa caaaattctc tatatccaac ttatctaggg
     3961 tctcaccttt agtgtacata tgtcttggtg aatccatatg agttccgttg tgagaaaata
     4021 cactaattaa tgtttcttga taaccatttt cttcaataat agctactttc ttgatatctg
     4081 gtctattagg ctctgcatat acaggcatgt cattatgtgt cacgtgagtt aaatcaaaaa
     4141 ccttcatctt tatatacccc ctatgttttt attttctatt atatacctaa attcatttta
     4201 tttctatcag attttattcc attatatacc atactagtat atatttttta taaacttcaa
     4261 aggatttacc gaaaaatctt ggaaagaatt atcagtaaat ataacatctt cacccaaaaa
     4321 acgtttatac cttccctctt ctttctttaa agtaattctc tgtttttgtg aatggttatt
     4381 aaaaaccatt atcttaagtg gaggtttttt tctattagtc tgaactcttt ttaacatgta
     4441 ttttatgtgc atgtcagctt cgggaaatga atatccacaa aatattaaaa ggtcagtatc
     4501 tcttaaagtt ttttctgtct cattccaaac attacttaag aatatattgg acatattctt
     4561 aaaatacgtt ggtggcacta taataggttc tgtcaattca ccacactcta aacattttgt
     4621 ttcagaacta ttttcaataa gtttcataac accaccttca tgtggtgtaa gagttacact
     4681 attacaaata ggacaataaa gccaattcaa tgacccatgt atcttatata atttgattaa
     4741 tggctctttc ggttttctcc aatttaattc ttctttaaaa tttgtaaact ccacaccata
     4801 atcaagcata attggcaaat cattcttatc tcttaagcca gtaactgtat tatctatatg
     4861 tatatcataa tttgcactga taaaagtagt atctaaaagc aaatcatttt tcaaaagatt
     4921 ttcaactaaa agtttatgat atttattatc tgttctaggt gtattatgaa ttgcctcagc
     4981 cattagcagt attaaatatt gtcttaaaaa acgtatacta tcacttctgt tattaagatt
     5041 ttctagtcca aaatttctaa atgcttcttt tctttgctct gctaagtcta aaagccctaa
     5101 aacttcttca aaggtaggaa aatttgcatt atctatatta tcttttacta tatcaatgtt
     5161 aaacatctgt ttaaagaaaa tatacaattc tctattcatt ttggtattat aattggtagg
     5221 atttagtttt ttaaaatatt cactaaacag ttcattttga ataggtagat tctctgctgc
     5281 tgaagctcct gctcctaaaa atattgctat tttcatatag ttcctcctaa tataaatatt
     5341 actcctattt ttatatccgt taaatatcca tatcatttgt tttatgatac aataataaag
     5401 ctgtccctta ctagaaaaaa tcctattaca agacagcctt atattatatt aaatctttaa
     5461 taattcataa atagaaagag catatatctg agcagccttt ataatatcct ctactattaa
     5521 gtattcatta gcttggtgtt ctgtttctgg ttttcctgga aaaatagctc caaatgcaac
     5581 acaattgtct aaagctcttg cataagttgc tcctccagat gaaagtggtg ttccatcaag
     5641 accagtttct tcttcatata cttttctaag tgtcttaaca agaattgtat cttctggaac
     5701 atagattgaa tccaagaaat catattcttc ataatttaaa ttgtatttat ccgtcatttt
     5761 ctttaattct tttacaaaat catctttctt gtatgttact ggaatcctta catctattcc
     5821 tgcaaattct ttttcgctat caatagttac tctacctata tttactgtta gtttacctga
     5881 cacatcatcc ttgcaattag gaattatatt gtttccatta gcatcttcac caataacttc
     5941 tgctaaaaat ttaattatat tagaatctat acctatatta ttaagagcta tacaaagtct
     6001 ggccactgca tttataccag tatcactcgc tgctgaatgg acacttttcc ctattataca
     6061 gattttatta ccttcaacag tatactcaaa atttaattta tcaagttctt tttttaattt
     6121 atcagagtat tttccaatat agattgcttt acctggaact gcatttagag cctttccaac
     6181 tgataattct atatcgcttt tgctatcaca agtcaaatga acttgtaaaa gacctttttc
     6241 tgcatttgtt attgggaatc tagaatctgg agtaaatcca tagtttggta tttcttcatt
     6301 attttcttta tatttattta tacatctcca aagattttct tcgtcagccc caaaaatgaa
     6361 tcttattctc ttattaaaat ctacatttaa atccataaga actttaactg cataaattgc
     6421 tgatattgta ggtcctttat catcttgtgt tcctcttcca tacaatttac catcttctac
     6481 aataccttca aatggaggat aattccaact ctctaaatca ccttctggaa ccacatcaac
     6541 atgaccaagt atacctatca ttttttcacc gtttcctatt tctgcatatc cataatatcc
     6601 atctttgtat actgtcttaa aacctagaga tttacataag tctaatatac ctcttaaagc
     6661 tttatctaca ttttcaccaa atggacaatt ttcagttgct tcagatataa cactaggtat
     6721 ttttacgctt tcttgtattg aagaaatcat ttcatcttgt agagaattta ctttttcttt
     6781 aatttgctct ttcatattat cacccttcaa ttttcatcat aatatactaa taaatatttt
     6841 gaccttttta aaaaaaaata tgcttatttt ttcagttttt ttataaattc aattgctttt
     6901 aaagtcccca tttttgaatg gtcaaatgta agtcctcctt ggaaataaac attataagga
     6961 ggtcttattg gagcatcggc acttaattct atagaagaac cttgtataaa tgctcctgct
     7021 gccattatta cttcactttc gtatccaggc atgtcccaag gaacaggttt cacataagaa
     7081 tcaactggtg cagcttcttg tataccttca caaaaactta ttacttcatc agcattatta
     7141 agtctaatac attgaataat atcacttctt aagtcatcgt attttggtaa tacatcataa
     7201 cctaattttt caaaagctct tgaacaaaag atagctccca ttacagcctg agatactatg
     7261 taaggtgcta agaagaaacc ttgaagtaca tttctagtag ttccaaaagt aaggccacac
     7321 tcttttccta ttccaggaga agtcattcta taagatataa gctcaattaa atcttttcta
     7381 ccagctatat atccacctgt caatgcaaga ccaccaccag gattttttat taaagaacca
     7441 gccataacat ctgctccaac atcagtaggc tctttggtat ctaaaaactc accataacaa
     7501 ttatcaacca ttactatagc ttctggttta acggacttta ttacctctat agcttctttt
     7561 atatcactaa ttgaaagcga ttttctccaa gaataccctt ttgacctttg tatcataaca
     7621 agtttagttc tatcatttat tttgttttta attccttcta aatctaagtt tccatcttct
     7681 aaaaaatcta catcatcata tgtaacacca tactctttca atgaaccttt ttcttctctt
     7741 atacctataa ctccctctaa agtatcatat ggtctaccag taacagataa aatttcatct
     7801 cctggtctaa ctataccttg tatacataaa gtaagtgcat gtgtaccatt tacaattatt
     7861 ggtcttacaa gagcatcttc tgtattaaat actttagaat aaatttcctc tattttttca
     7921 cgaccaatat cattatatcc atatcctgta gtccagttaa agtgcatatc acttagttta
     7981 gattcttgca tagcttttaa aactttatat tgattgtatt ctcttatttc ttttatttct
     8041 tcaaactttc cttttatatc ttccattatt tcttgagata gtttaaaagt atcatcatct
     8101 atcccatagt agtctttcaa taattctttt gtctcattaa gcattttatc acctgtctct
     8161 ttattgtttt atttaaatat gtaaaggaga ctattttact aaaacaaata aattatagtt
     8221 tatcagttag tataagtctc ctctcaatta tcttaaatta aattagttat ctatctgttg
     8281 ttattattat tattttgatt ttgatttgtt agattaatat actttccagg ttgtatagtt
     8341 gatacagcat gtttataaat catgttttgt tgtctattat ccccttctat taatattata
     8401 taactatcaa acccctttac aagcccttta acttgcactc cattgactaa gtatatagta
     8461 acgggtatcc tttctttcct tgcattattt aaaaacaaat cttgtaaatt taaaactgta
     8521 tttttcatta gatttgtacc ctcccaagat taaattgaac ttctaaaata ttcctacctt
     8581 atattctata taagttatga caaattttca atattaagtt gtatacttaa aaatttatat
     8641 attattttat agaatcttta atatataaga ttatttcatt ttttaattcg tctatatttt
     8701 catattgctc taaatcaaac cactttgctg ttttatatct tttaaaccat gttatctgac
     8761 gttttgcata ccttctagaa tctcttttaa ttatttcaat agcttcttca tatgtatatt
     8821 ctccatccag atactttaat atctccttat agcctatacc ttgcattgat atcatatctt
     8881 ttttaaatcc catacttaaa agttttttta cctcttcaac caatccattt ttaagcataa
     8941 tatcaactct catatttatt ctttgatata aatgttctct atctctattt agaactatta
     9001 ttatcggttg atattcttca ttaaatttta aatcattgga aaaatcattc attttcttgc
     9061 cacttaggca aacttcaaga gctcttatga cccttttagt attgttttta tggattctat
     9121 tagcagattc ttcatcaagt tcttttagcc tattatgcat gtaatctatt cccttttcag
     9181 caagttgatt ttgaagttct tctcttaatt cattattagc atcagactgt gcaaaatcca
     9241 tattatatat taaagagttt aaataaagtc ctgtgcctcc agttactaaa acacattttc
     9301 ccttttcatt tatttcatat atatattttt ttgctctttg ttgaaactct gaaactgaaa
     9361 atggatagtc cggtttaact tcatctataa ggtaatgctt tactccttgc atctctgcct
     9421 ctgtaacttt tgcacttcca atatccatat attcatatat ttgcatagaa tcagctgaaa
     9481 ttatctctgc attcatatct tttgctaatt ttattgacaa atctgttttt ccaacagcag
     9541 ttggacctgt aagtattata agtggtattt ttttcatagc attcctttcc atatatttta
     9601 cataattctt ttaaacattt tctctatttc tgtcttagat atttcaacca ttataggtct
     9661 tccatgagga catgtaaatg gattctcaca cttttctagc tgttccaata aactttttat
     9721 ttcaatatca tatattttgt catttgcttt tatagcagac ctacatgcca tagatgcaaa
     9781 cctctctcct tttaaatcat aattacttgt aatttcttct atattatcaa ttatttgaag
     9841 tataaatttc tcagtttcag gcactccaaa tatagtagga acacatctta ccattatatg
     9901 attatttcca aatatctcta attcaaaacc aaatttcata aataactcca aattattttc
     9961 aatttgaagc atatctacat ttgatacttc aataatcact gggtctaaaa gtatttgcat
    10021 attaatatct tgtctataga acttctccat atacctttca tacagtattc tttcatgtgc
    10081 agcatgttgg tccaacaaat acatagaatc gtttttgctc aaaattatat aggtattaaa
    10141 tacaactcca attacagaat atccatataa ggaaaacttg cgttttgagc tacacaagta
    10201 ctcttcttca ctatccttaa tactatctcc taagtaataa tttccttcat ttctaattcc
    10261 atcaacttga aattcttctt gtatattgtc atttgcactg tcttcactta ggtaattaat
    10321 gttatttact tcatcttcac ttgcatttaa gacttcactt actgattgaa attcatttat
    10381 tggtttttca ctcgataaag ttacaacttc aataacttca tccttacttt tagtggtatt
    10441 actattttta ggagtagatt ctaataaatt atcatttctt aatttataat ctgtactttt
    10501 ttctctactg ttaatagcga ttctaggctg tgttttttta tcactataag tagcatattt
    10561 tccaataaga tttgagtgta ttaacttaac ctttaaaaaa tctctaagtt ctatatacac
    10621 ttcttgttct ttttcaaatt taatttcaag tttattggga tgtatattta catctataca
    10681 agatggatct acttctatat ttaagaaaca aactgcatgt tttcctattg gaataataga
    10741 tttataactt tctgttattg catctattat tattttactt tttacaaacc ttttatttat
    10801 atagatatgt tgtaggtttt tattagaacg gtatatatta ttattaccta tgtatccatt
    10861 cattttaaag tgattgcact taaattctac atctattata ttctctgtta tttcttttcc
    10921 atatatactt ctaatcgtat ttacaagctt cccatctcct ggagtattta gcatctgctt
    10981 attgttattt gtatatttaa attgaatgtt tggattacct attgcaagtt tatttatcaa
    11041 atcacttatg tttatagttt ctgcatgagt agattttaaa aatttttgtc ttgctggtgt
    11101 attaaagaaa atatctctta taatgattgt agtcccatta gtagagccta tgggttcttt
    11161 tgatattatt tttccacctt caacatatat tttagttcct attatttcat ctttagtttt
    11221 ggttgtcatt tctaattttg atacagctga aatagatgcc aatgcttccc ctctaaaacc
    11281 aagagagtat aaatcataca aatcatcaat ttttttaatt ttactagtag catgtcttaa
    11341 aaacgatttt tcaacttcac ttgaaggtat tcccattcca ttatctgtaa tcttaattag
    11401 actttttcct ccatcaatta tatctataga aattttattt gcacctgcat caatggagtt
    11461 ttcaataagt tcttttacta cagaagaagg tctttctaca acttcacctg ctgctatctt
    11521 atttatagtc aaatcatcta ggatgttaat tatatttttc atttttacac ctctaagatt
    11581 ttatttcttt tgcttttctt tgaagattat aaagagagtt tatagcatct aatggagtca
    11641 tatttaaaat atccagattt aaaatttctt ctgatagtat gtctctattt acactatcaa
    11701 aagatatttg agttaaggct acttccttta cagaatcatc ttggttagtt ttctctttat
    11761 catcattttg cttcattaaa gcaacttcat catttaatac tttaaattta gaatttaatt
    11821 tatcataatc cttttttata tgcttgtagt cttttacgat aagttcatgt tctattttta
    11881 gagattcata tttattctta aaattacttt gatttacctt aactaattct tcgtctaaat
    11941 tactattaat tatattatta ttttctttat ctccatttat tgcaacactg ttgtaaacat
    12001 gatttttttc caagtcttta agtatatatt ttgctctttc tataacttcg tctggtaatt
    12061 tagccaattt agcaacatat attccataac tcttatctgc tccctgagga attatttttc
    12121 ttaaaaatat tattccttcc ccatcttctt tgactgctat tgaataattt ttgacctctt
    12181 taaactcttc ttctaaatca gtaagttcat gatagtgtgt tgcaaataat gtcttgcatc
    12241 taatgttttt ttgaatatat tctactattg accatgctaa acttatccca tcataggtac
    12301 tagtcccacg acctatttca tctaatataa ctaaacttct ctctgttgca ttttttaata
    12361 taagggatac ttcgttcatc tctaccataa atgtagattg accttgagat aaatcgtcac
    12421 tagctccaac tcttgtaaat attctatcta gtattggaat atcagcatac tctgctggta
    12481 caaatgagcc tatatgtgcc ataagagcta ttatagcagt ttgtctcata tatgttgatt
    12541 tacccgacat attaggacca gttattatgt taataatgtt ttctccacga tttaaataag
    12601 tatcatttgg aacgaaattt tcctcaccta ctatattttc gacaacagga tgtctaccat
    12661 ttcttatatc aagtttatta ttttcattta tcgctggttt aacatagtta tttatatgtg
    12721 caaccgttgc aagagagaca aagacatcta tattagctat agtcttagct actttttgta
    12781 ttctatctat gtttttatat attgtatctc ttatctctac aaatatctca tattctaaag
    12841 atttaatctt ttcctctgca tgtagtatct tttcttctat ttcctttaat tctggagtta
    12901 tatatctttc tgcattacta agtgtttgtt ttcttatata tgtctcatct agctttgcct
    12961 gtttaaagtt agcttttgtt atttctatat aatacccaaa aactttatta aaccctattt
    13021 ttaatgattt aactcctgtt ttttctcttt ctctgttttc tatctccttt acaagaaagg
    13081 ctccattctt agaaatctct cttaattcct taagttcatc actaaaatct gatttaatta
    13141 tgtttccatc ttttatggtt attgtaggtt cttctaaaat agcttcatct attaaattgt
    13201 atatatcatc taatttatcc attcctgaaa catactcttt taggattttt gcatttgata
    13261 gatttatagt atcttttaaa ttaggtaact tttctatcga attttttaag tgaattaact
    13321 cttttggtgt tactctctca aatgctatct ttccacatat tctttctata tcgtatatat
    13381 ttttaagtat atcatttagg tcttctctta atataaagtc atcttttatt tcttctatta
    13441 catccaatct attttcaatt ttagatttgt ttatcaaagg ttcttctaca tattttctta
    13501 aaagtctacc tcccattgct gtagaagttt tatcaagtac atgtaataat gaaccttttt
    13561 tcttattccc tcttatagtt tgggtaagtt ctaaattagt cctagtaaac atatctaaaa
    13621 ccatatattc tgatgaattg tatatattta tattattaat gttagatgtt atttgttttt
    13681 gagtattgta tatataattc agaagaattg ataagcttga ctttattaga cctttgtcat
    13741 caaactttag tttttgtaga taaacatctg gaaaatattc ttttaatata tttatgtcca
    13801 aatagttatc gctaaaactt tcatttatgt atatattact tacagtagct atatctctaa
    13861 gcttttctat aaagtctaaa tcatttataa ttatttcagt tggatggatt tttgcaattt
    13921 cctcaattac cttatcttca tttaaacatg ttgcattagt ttctcctgta cttatatcaa
    13981 cataagttag acctatatta gtaccatcct tatacaaaga taacaaataa ttattctttt
    14041 tattttcaag taaatttcca tctaaaactg ttccaggagt gatgactctt ataacctctc
    14101 ttctaacaat tccttttgct gtagatgggt cttccatttg ttcacctatt gctactttat
    14161 atccattttc aactaatttt gatatatatg aattggctga gtggaaagga acaccacaca
    14221 taggagcacg ttcttctaaa ccacaagctt ttcctgtcaa tgctatttca agagcttttg
    14281 aagctactaa tgcatcttca aaaaacattt cataaaaatc tcccagtcta aaaaacagta
    14341 tacagtcttt atatctattt tttacttcaa gatactgttt catcatagga gtcaatttat
    14401 tcatatctat ttgcattatc aaacctcctt cttttctaat taaatattat atcatagtag
    14461 atgattatta ttaacacaat atataaaatt ttgacgtata actaaaaaca atccaaaatg
    14521 tatactcaca ttttggattg ctaagtttaa tacttattaa ataaaaatat atataatttt
    14581 acaataaaga tattatttct tttttaagtt taatgatgtt ttcaaggtta tcttcattta
    14641 aatttctact atctataaca aattctttct ttactattga tggttttttt gaaataacat
    14701 aaattctgtt tgatagcatt atagcttcgt caatatcatg agttataaga agtattgttg
    14761 aatttacttt tttcttaaca tctaaaagcc atttttgcat agatgtttta gttaatgaat
    14821 caagtgcgcc aaaaggctcg tctaaaagca ttatgtcatt agaatttata aaagttctca
    14881 agaaattagc tctctgtctc attcctccag aaagttctga agggtattta tcttcatatc
    14941 cacttaatcc aaatacctca aaataaggtt ttaccattga cctagcttta ttcctttttt
    15001 ctttttttaa aactagtggt agtgatacat tatctattat agttttatat ggcaagagta
    15061 aatctttttg atacatatag ctatatttac catttatatt cactttgcca ctatcataat
    15121 ctgtaagttt agttataata ttaaaaatag tactttttcc acatccactt ggccctaata
    15181 tagaaacaaa ttcaccttca taaacatcaa tacttatatc ttttaaaact tcaatatctt
    15241 tataagtttt tgatacattt tctatagata cttttacttt tttattttgg taaaaattca
    15301 tttgtgtaag catcacttgc cttcatatcc ttatctatta atttatattc ttttaagaat
    15361 gatgtatagt tgttccatac actatctttc atttctcccc atcgtggtgc atcatctttg
    15421 tatttgcttg ctaaatattc ttgactcttt aaggctagtt tttcatctac ttcaggtgca
    15481 tgttttacta aaatttttga tgcttcttct ggatttttta ttgcatattc ataacctttt
    15541 gttgtagctt ttaagaattt tttagcaagc tctggattat ctttaagcac agtttcattc
    15601 gaaattataa gtggtgtata ataatccaat ctctcatcca aatcttttac tggtataaaa
    15661 tttaagtcgt atccaattaa atcagcttta actgcatccc aaccttcaaa tatccatgca
    15721 aaatctacag ttttcatagc tgcaaagaaa tcatcttgac cagtattgac tatttttaac
    15781 ttattaaaat cagctccatt tttcttcata actgctttaa aaacagcttc ttctgatgga
    15841 gaaccccaac caccataagt ttttccttca aaatctttag ctgttgttat attcttttct
    15901 tttggtgatg caaatccaga agtattatgc tgtataacag ctgctacagc ttttattgga
    15961 agtgggtctt cactagtctt tgcataagta acctgctctt gataacttat accaaagtca
    16021 gcttttcctg ttgctactaa agtctcggct ccactttcag gtggttgtac tatttcaaca
    16081 tctaaacctt cttctttata ataaccttta tcaagtgcta caaataatcc agtatgattt
    16141 gtatttggtg tccaatcaag taccatagtt acttttttgg aagaagcatt tttatcattg
    16201 tttttatctt tattattatt tgaagagcat ccagttattg ttgccaatgt aaaaacactt
    16261 actaaaccta atattcctaa ttttttaaaa ttcataattg cctccctcaa atacctttaa
    16321 tctgttaatc tatgtcttat aacaattttt tttacaaact gtacaattcc tacaaacatt
    16381 aaactaaata taactactaa tatagtagat gcaaatactt tatcaagtgc atatgcactt
    16441 tttgacctaa gcatataaac accaagtcct ttcgttccac ctagccattc tgcaacagtt
    16501 gctgccatta cagcatacgt tgcagatatt ttaagtccag aaaaaaactt atccattgcc
    16561 ataggaaact taaggtgaat gaatgtatta actttatttg agttcatagt cttaaataaa
    16621 tttaaataat ctttatcaat attctcaata ccatctacaa aacttattaa tataggaaaa
    16681 aaacaagtaa gcattaccat caatactttt ggcattgttc caaatccaaa ccatatcata
    16741 aaaagtggtg ctataattat tgtaggtatc atctgagtaa ccaacataat aggataaaga
    16801 cattttttta ttattgatac aaaatccatc aatattccga ttaccaatga tattataatt
    16861 gcgactataa aaccaagtac tgcttctcct atagtaacca cagaatgcat ggataaattt
    16921 tgataatctt taaaaaacac attaattata tctactggtg ttggtagtat atactgagga
    16981 acttctaaaa ctcctaccac agattgccaa agaataagta taaacaaaaa tgttgctatt
    17041 ggatatatac tatctttgac tttatctatg atttctaagt ttttcatcta tagttagacc
    17101 tccagctttt tcatctactc tagctactgt tatgactctt tcacatccaa gattaaaagg
    17161 gattttatgt acctttttta ataattcaaa aacttcatca aaatcacctt caacagtagt
    17221 gctcattgct cctatttcac atttaagacc acttttttca atttcagcaa tacaagcatc
    17281 tactacttta tacatttctt cttcaccttt atagggtctt agtggcatta ctgctatgtc
    17341 tgcgattacc atttaaatca ctccttttaa aataattaat acctactagg tatattttgt
    17401 tttttattaa aaaatttttt ttactgaaaa gatattatat ttaaataaaa gaaaagtctt
    17461 atccaaaata ggataagact tatttttatc ttaactggtc tccctacact ggcattatcc
    17521 aaacaggttc aaagggtcga gataaaatct cctctcagca cattagctcc ccgaaatata
    17581 ttatatcttt tctacttgat tatactacac ttatcatatt ttaacaaatt ttttatttat
    17641 attagatatt ttatctgttt aaaaatatct aatataaatt gcacattatt atatcaaata
    17701 aattatctta aaacgataat ttctcagtat ctcctaaaac tagattaacg taattttcta
    17761 atttttgttt tactatttca agcatatttg gatatatatc aattccatcc aaacattgtg
    17821 ctaaattttt atttttgttc atttaagtta caatcattac tgattaagca tagtttttat
    17881 actacactca tctttatata caaaaattcc ttcaataaat tccttaaaat tatattctaa
    17941 ataagtgatt tcatagtcag attcttgatt aatctctact acacagggtt cttcttgtgg
    18001 ttcacaacca ctatagtaaa gaaatatcat ttcgtgccca ccagaaatgg tattatatat
    18061 gaccatacct atatagggtt aacacattct actatccaaa aacgactgcc agcattgcta
    18121 cataaggaat atgttttttc tgcgtcaatg acatatagac tctctaagta gaaaatgtta
    18181 gatgccattc gttttgtatg gtattatcaa attttttttt agatttttat ttcttcttat
    18241 ccttgtccat tattcctttt atgacatagt atgcttcgtg agtgaataat tgtaaataaa
    18301 taatattcat taagctaaaa atagacttct atctggattt tcaatatata aaccttttag
    18361 cagtattttc tgtgataata taaatattac tcgtccttat aatcaaaatc ttcagcaatt
    18421 ggccttttat ataatataat tattttattc aatttatttt tgaaaaataa aatatttttt
    18481 taatttttaa aatctttaat atgtagcttg aaaaacaaaa cgttttccta tatgatggta
    18541 atagtagaat aaaactaaat aggcaaaact agagaaattt agtgacgcaa agctataggg
    18601 actaagactt atgaaatgag ttatgtcagc cagttgccaa agagatattt tctttttgtt
    18661 tttatgaaag ttttttaggg ggatatatga tatatgttta tggataaaat ggatagatgt
    18721 acacatatac taactgcata tatatgcagt tcacgcgatt actgcaattt tatagacaca
    18781 caattaaatg attttatact agaatatgga gagaatgtgg tagaatcttg tttgcatcag
    18841 gtgatggtat tggtaagcag atataattaa agaaatttta ttttctagaa aacccttttt
    18901 agttttatct acgttttata attcattaaa aaactaatta aaataagctt cattatctat
    18961 ttttcatgaa gtaaacagtt gagtttatct catatactgt ttaccccaat tacataattc
    19021 taataatata gattccaatg atttaccacg tttagtagac aaatattcaa cttttggagg
    19081 aatagtagga taagaaatcc tcttaataag tccatcgctt tcaagatact ttaattcttg
    19141 tgacagtatc ttatgactta tatgaacaac ttcttttttt aattgactat aacgtattgg
    19201 ctcttgtttt aaaatatgcc ataaaataat tgccctccat ttacttccta tcacagtcat
    19261 ggtaaaacca attgaacaat taaaatcttt ttgtctttct tcaatatttg gaaattcttc
    19321 aaaattatta tctatatcca tctcaatcct ctctttcaaa atagtaagta tctaactaaa
    19381 aagtgcattc ttttttaatg tactaatttg taatataatc ttgattgtag catagaacct
    19441 tacataatac tactttttta gatgtatatg tttaaatttt aatatagata tatgttattt
    19501 tattaatttt atttaaacag ataataataa ataataattt atctcaagtt ttctatgtac
    19561 ataaactaat ctaatgttta gaaaggatta tataatatga ataaaaaatt tcgtctttat
    19621 caagttgact catttacaaa agaaaaattt acaggtaatc cagctggtgt aattacaaat
    19681 gctgatggct tatctgaatc acaaatgcaa aaaatagcaa gagaattaaa taattcagaa
    19741 actgctttta ttttcaataa attggctaaa aatcatcaag aaaattatga tgtacatatc
    19801 agatttttta caccaactaa tgaggttcct atatgtggtc atgctactat tgctgcccat
    19861 tatgttagag cagtagaaaa ccaactcaat aacaaaacag tatttcaaaa aactggtgct
    19921 ggaatattac cagttgatat tataaaaaaa gataatgatt atgaaataat tatgacacaa
    19981 ggaaaaattg aagtatctcc accattacaa caaacctatc aagaaagatt acttaatgca
    20041 tttggtattt taaaggaaga tattcgtaca gattgtccta ttgccatatc atctacagga
    20101 cattcaaaag ttatgattgg aataaaggaa ttatcaacat taaatagttt atctccaaat
    20161 ttatatgaat tatcgaaaat aagtcaagaa ataaaatgta atggatatta tatttttaca
    20221 ttacatgata aagaaactct acaagttcat ggaagaatgt ttgctcctgc aattggtatt
    20281 tcagaagacc ctgttacagg aaatgctaat ggacctttag gtgcgtacct tgtacattat
    20341 aaagtaatac ctatacccaa cacagagttt atatttgaag cagttcaagg agaagcaata
    20401 aaacgtaaag gcaagatgtt agtaaaagta attatttcag ataataagcc agttgaagta
    20461 aaaataattg gaaaagctgt aattgcattc tcaacaaaca tagaaattga attataggtt
    20521 ccttctaagt aaatttttga actttttaat actaatttaa taacaaacta atttatatac
    20581 aatcaaatta atttttttat tatttgttaa tatatattta tcattatatt atataat
//
LOCUS       DAMJVM010000061        20561 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.064, whole genome shotgun sequence.
ACCESSION   DAMJVM010000061 DAMJVM010000000
VERSION     DAMJVM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20561
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.064"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            59..1015
                     /locus_tag="PC523_002387"
     CDS             59..1015
                     /locus_tag="PC523_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HDF2475901.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYSFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFVGIIIFKIKKLL"
     gene            1477..2490
                     /gene="aroF"
                     /locus_tag="PC523_002388"
     CDS             1477..2490
                     /gene="aroF"
                     /locus_tag="PC523_002388"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDF2475902.1"
                     /translation="MIVVLKIGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKIVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEEQIVSIAEDVKKSGAGFLRGGAFKPRTSPYAFQGLRYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            2512..3576
                     /locus_tag="PC523_002389"
     CDS             2512..3576
                     /locus_tag="PC523_002389"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDF2475903.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILNNVGEEIKRIYFGNKIFIVTD
                     ENVAKLYLDKLKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGINFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKHYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVEAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            3576..4889
                     /gene="aroA"
                     /locus_tag="PC523_002390"
     CDS             3576..4889
                     /gene="aroA"
                     /locus_tag="PC523_002390"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDF2475904.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSIAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGVKRFIGRGNLGKRPLDTYYEIFNRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTMKDFGIEI
                     INNNYKEFIIKGSQTYKARDYKVEGDYSQGAFYLSADAIGEDINILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNSTLIDASQCPDIIPVLSVVAALSRGKTTIIN
                     AGRLRIKECDRLHAINIELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFEMLGGRIDEWNMGK"
     gene            4867..5946
                     /gene="aroC"
                     /locus_tag="PC523_002391"
     CDS             4867..5946
                     /gene="aroC"
                     /locus_tag="PC523_002391"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDF2475905.1"
                     /translation="MSGIWGNNLKISIFGESHGNAIGINIDGLPSGVELDLEKIDREM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEVGAHIKKIK
                     NIEDMSFNYVNISKQQLSNLQTLELPLLDLSKEEKMKNTIIDAKNQRDSVGGIIECAV
                     VGVNVGFGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKEKKDDILSIKGRHDP
                     CIVQRVIPVIEAVTAIGIFDLMKGR"
     gene            5949..7145
                     /gene="pheA"
                     /locus_tag="PC523_002392"
     CDS             5949..7145
                     /gene="pheA"
                     /locus_tag="PC523_002392"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDF2475906.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETIQFFNSIMEISRGLQKRLMDNDSEQKIEFRLDS
                     SKKKFDLSSVNKYKSLKEELNKKNILVGFPGKSGSFTEEALNKFFNKKTAKKQFKEFE
                     DVFIALKNKKIDYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLKDIKEIYSHPQGIGQCSEFLKQNSAWKLIPFNNTATSAELVKKLQDKTKAAIASKK
                     AASIYGLEIISPCINDITNNYTRFVVISNQIHIEEESNKMSVVFSVEHKAGKLYKVLG
                     YFAENNINMTKIESRPMKNTSWRYFFYIDFECSIYNSKVYNLLELIELNTAYFKFMGV
                     YKNKTK"
     gene            7185..7994
                     /gene="aroE"
                     /locus_tag="PC523_002393"
     CDS             7185..7994
                     /gene="aroE"
                     /locus_tag="PC523_002393"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDF2475907.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDVS
                     KLASAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLREDMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAAITYLLDSGIEKVYVSTRKKDDKKLLNS
                     KAILVDYEELKCITGDIILNATPVGMYPNVGISPVSKSIIQNFDTLVDLIYNPGETEF
                     LRIGNSIGKKTCDGLYMLVGQAIKSQEIWQNAKIDNSILDVIYNELKLEFL"
     gene            8006..8539
                     /locus_tag="PC523_002394"
     CDS             8006..8539
                     /locus_tag="PC523_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDF2475908.1"
                     /translation="MTNKTKEKLILIGMPGSGKTTIGKLLAREYNCSFCDMDDYIVKI
                     AQKSIAELFSEGEDIFRNYETQACRELSISNKAVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNTAVY
                     NIINAVSEKFKFEFKEK"
     gene            8560..9399
                     /locus_tag="PC523_002395"
     CDS             8560..9399
                     /locus_tag="PC523_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDF2475909.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKRGIDFASEQVFNGANYIITPTGRNNIRNLELVENLI
                     LKLGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEDRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLIKEAIFNNDKSKLIEYFKKSSIRREI
                     LEK"
     gene            9678..11147
                     /locus_tag="PC523_002396"
     CDS             9678..11147
                     /locus_tag="PC523_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2475910.1"
                     /translation="MLIVRNVSIAFIVLFFFSVFAFFYVANINRVLEYETNNLMTITI
                     TGWIILSFLFLGIIIYILYSKAKSQKTIEKVAYTDFVTGYSNWRKFELDVTNLLKKTS
                     QNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELININETFARVSADNFNI
                     LLTYNKKEDIINIIKKIMVNNELVNLSFGIYEIKDKALSVSVYSDRASLAKSSIKNNS
                     DVNFAFFNDELREKLLLEDKIEKEMEYALESGQFVMYLQPKYNIKLNKFCGSEALVRW
                     QYTEKEVIYPGDFIPVFEKNRFIRKIDMYILEQACKEIRSLFDKGISPLPISVNFSRV
                     DFLKKDFISNIVNVCDRYKIPYGLIEIEITESSMFGDTDILFNVSRNLQDIGFIVAMD
                     DFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRVVAEG
                     IETKSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            11180..14617
                     /locus_tag="PC523_002397"
     CDS             11180..14617
                     /locus_tag="PC523_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF2475911.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSMANMVSYFNTIDNSKIIPLLANISKESDFQRISIV
                     SPDGISHTSDGKIIDVSNYQYFKKAISGESNISEVVKSPIDGKDTFVIATPIFNKNNK
                     IIGILYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYKDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIILIIYIIYKNNKSQRDILKANKEISSITNSIPGG
                     VQKCTLGETCKINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEISKQL
                     YKNKTIELEYRIITKSGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENILGGILITELNEDFTIKYTNSGYLSMIGYTREQLENELKYKACDLVFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIIDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITENV
                     PYSLVESGVIHKDFSNDFINMYEKVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNENW
                     QKPFGIKPDNNYSQMAKTIANSIVHKENKEVFLNTFLCENLLDAYKNNITEIKLEYQR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYN
                     KGTTEKLINDFLNSEKASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKHLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENAKKKAEKICDIFRNSYTKNKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKVANK
                     CEKSNPVLKEVLDSIDNYIYIVNPNTYELLFVNNKVSRSGLEIKLGQTCYKSLLKSDR
                     PCSNCPLNGLEENKHSKFKIETNTFGTNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            14634..15380
                     /locus_tag="PC523_002398"
     CDS             14634..15380
                     /locus_tag="PC523_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDF2475912.1"
                     /translation="MLNGITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIILIIYQ
                     IFKKKIKEKKVFYRVLKIIISVFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TIPSTTLKGRLNTALKYLKVNDNCYVVVSGGKGSGENITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKFKIEERSERKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIFFDR"
     gene            16260..20426
                     /locus_tag="PC523_002399"
     CDS             16260..20426
                     /locus_tag="PC523_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HDF2475913.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANVPVVLQDIATGVRLTVL
                     TNASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFAAAIVGPVPIAEIPPISSVINPP
                     IGSTNLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIQNILDSCVSVSNINLIIDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNFLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEIGTVINSQNNNQLTVEFFS
                     EGESVIGNDYAIDDVSLREITVPTFTPIKTSNTETANIGDIVTYTVTLDNTCNSPLTN
                     VFFRDIIPNGLKFIPGTVTVDGISVPGVDPNIGFQLPDIGGGTRIEVTFNVVVESIPN
                     PNPTNNIATIDYSYTPVEGGIPNNFSVDSNPVPVEVISADIEVTKLSEPSIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPDSIINSEYSLDGGVSFQPWNGSLNIGTLELGATR
                     VIIIRGIVNPTTVGIIRNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVANLGPANAENVILNDDIPDVILNPEYSLDGGVSFQPWNGSLNIGA
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVANEDVAVPGEEFVYTIEIFNEGPSNAMNVVLTDNIPDVILNPEYSLDGGASFQPWN
                     GSLNIGTVAPGQLIRIIIRGLVSSTATGNITNTAEVSAEVPEPVTDSSTVITPVVASA
                     DIEVIKTSNMDTAVPGEIFSYTITVTNLGPSAAQSIVLTDDIPDIILNPEYSLDGGVS
                     FQPWNGNLNIGTLDTGEIRSIIIRGTINQTSVGTIINTATVSSTTPDPNPDNNTSTVE
                     TDISPLADISVIKGNEPLAIQGERFIYGIEIVNAGPSFSENIVLTDNIPASILNPEYS
                     IDNGVTFQPWTGSLNIGTLDAGEIRNVIIRGTVSQTAVGTIINTATVSSTTPDPNLNN
                     NTSTSEAEVSSSADISVVKRSNQNVVVPGDVLNYTIEVRNAGPSTAQNVTLTDNISAS
                     ILSPEYSIDNGVTFQPWTGSLSIGTLYAGEIRNIIIRGIASETAIGTIINTATVSSTT
                     PDPNPNNNTSTSEIDISSSADISIIKLANKTEACVGEQIDFVIVVSNAGPENAQNVTL
                     IDNVSDKLKKTVFSLDRGVSFQPWTGSINIGILPAGTLRVILLRGLIKSTCLDKLTNI
                     AEVTSTTPDSDLNNNISRVQVEIKQCCSNDCCCNCCCNNCCNCFFNDCCNDCGYKDCC
                     CNCCKNDFWC"
BASE COUNT         8377 a         2230 c         3432 g         6522 t
ORIGIN      
        1 ttacagtaag gattataaaa aatttttaga atgtaaattg aaatttagga gtagatgtat
       61 gtatatactg aatttaaata caagagagac tattgaagat tttagagata aattttatgt
      121 agcagaaaat tcttatctta tattaagtgc tccaaaaaat ttaaaattgc ttaaagaaac
      181 attggatata gatgaaataa cttttaatga ttgtcttaaa tttgatgaaa taaccaaact
      241 tgatttattt gataattacg attttttaag cctaaataca tttgaattaa gagatggtga
      301 agctgttata gaagaggtaa atatgtattt gtctgataat tttatattgg ttgtagttaa
      361 tgaagaacac tttttatttg aatttgtaaa aaatattatt ttgaagaact ctcaacttga
      421 aaaaaatcca gttattaacc tatttaagat aaactacttg attctcagag aagttataaa
      481 aaatggtttt gagtctttgg aaaaagttga agagttgatt cttcaaatag aagatgaaat
      541 gatggacaac attaacaaga atcatgtatc tagaataagt gatgtaagag gattaacaag
      601 gattatagtt aagaatacta gacctctttt gtatattggg gatagaatag ttaaagaaaa
      661 tataagatat ttaaaatatt caaatgtaaa aaaatataat ttagaaaatt ttcaaggtat
      721 agattttgga attgataaac tatacagttt cgcattgtca acacgagagt tagcagataa
      781 attattagat atttattctt ctagagtagg tgaaaaaaca aataatttaa ttacaaaatt
      841 aaccttatta acagccatat cagcaccact tacaattata actggtatat atggaatgaa
      901 ctttagatat atgccagaat taaactggat ttatggatat ccagcaactt tattttttat
      961 gttatgtata atttttgttg gaattataat atttaaaata aaaaaattat tgtaaattac
     1021 agatgatttt taattaattt tataaaaata catcaattga gaagccgatt taagtaaata
     1081 aaaaaatatt ttaaattgaa aatatttata gaaaatattg acaattgtat tttctttgtt
     1141 ataattagta aacaagaaga ataataaatt ataaaaatgg aggataatac tatgaaaggg
     1201 atactaaaag aactagcagc aaaactttat agcttttttt actttatgtt ctggggcttt
     1261 tattttagcg ctggctattt cttttacaaa aatatatgta attcctttct ggtgaggcct
     1321 tattttagta attttgagca taagtaaatt ttataaaaat gattatttta tatatttatg
     1381 taagatttaa tgcttgatag attattttag agaactcatt agtgagttct cttttttatt
     1441 ttaaatataa aattataata ttgggaggta gtaaaaatga tagtagtatt aaaaatagga
     1501 gcagataaaa atgaagttaa aaaattaatt gaggctattg ggagagaagg agtagaagtt
     1561 aatcccatag atgggacaga attaactgtt ttaggtttag ttggagatac tagtaaaata
     1621 gatgccaaaa gaatagaagc aaataagata gttgaaaaag ttatgcatgt agtagagcct
     1681 tttaaaaagg ccaatagaaa atttcatcca gaaccttcta taataaatgt taatggaatg
     1741 gaaattggtt cgaaaaaaat tgctatgata gctggacctt gttctgttga aacagaagag
     1801 caaatagtat ctatagctga ggatgtgaaa aaatcaggtg caggattttt gagaggtgga
     1861 gcattcaaac caagaacatc accatacgct ttccaaggat tgagatatga tggattggac
     1921 ttattaaaga aagcaaaaga aaaaacaggg cttcctatag ttacagaaat aatgtcaaca
     1981 caagatatag atatatttga agaaaatgta gatgtaattc aagttggagc tagaaatatg
     2041 caaaactttg atttattaaa agaattgggt aaaacaaata aaacaatact tttaaaaaga
     2101 ggattatctg ctacaattga agagtggtta atgtcagcag agtatataat ggctggagga
     2161 aatgaaaatg tagttctttg tgaaagaggt ataagaactt ttgaaacata cactagaaat
     2221 actttggatt taagtgcaat tttagctgtg aagaaactta gtcatttgcc agttatagtt
     2281 gatccaagtc atgcagcagg caaatcatgg atggttgact ctttatctaa ggcagcaatt
     2341 gcagttggag cagatggtct gataatagaa gttcataatg acccagcaca tgcactttgt
     2401 gatggaaaac aatcaattaa accaaatgaa tatgatgaat taataagtga attaaagact
     2461 attgcatcag cagtaggaag agaaatataa tctataaaag gatggttaaa cgtggaagaa
     2521 aaactattag taaatttaaa agaaaatagc tacaatatat ttattaaaaa aaatatacta
     2581 aacaatgtag gagaggaaat aaaaagaata tattttggaa ataagatttt tattgtaact
     2641 gatgaaaatg tagcaaaatt atacctggat aaattaaaaa ataatttaag tgaatctgga
     2701 tttgaaataa aatctttaat attaaaatct ggtgaagaaa caaagtcatt taatactctt
     2761 cctaaaatat acgatagttt acttgacttt aagttaacta gaaaagattt gattattact
     2821 ttaggaggag gggtaatagg tgatttaggt ggatttgcag cagcaacttt tttaagaggt
     2881 ataaatttta ttcaaattcc aacatcttta ttagcacaag ttgatagttc tgtaggtgga
     2941 aaagttggag ttgaccttga gagaggtaaa aatttagtag gaagctttta tcaaccaaaa
     3001 gctgtattta ttgacccaaa tgtattatta tcacttccag aaaaattcta tagagatgga
     3061 atggcagaag ttataaaata tggatgtata aaagataaaa ctttttttaa taaactttta
     3121 agtttaaaaa atagagaaga agtaatgcaa catatagaat acattataca taaatgttgc
     3181 tttattaaaa aaactgtagt tgaagctgat gaaaaagatt ttggggatag aatgctttta
     3241 aactttggtc atacattagg tcatgctatt gagaaacact acaattttac aggttatact
     3301 catggagaag ctgttgccat aggaatgtat aagataactt tagaaagtga aaagatagga
     3361 attacaaaaa aaggtacatc agaacttata aaatcaattt taatgaatta taatttgcct
     3421 tatgagattg aactaagaga taataaatct atagtagaag ctatatctct tgataaaaaa
     3481 aatctaggtg atacattaaa aataatactt ttaaaagata ttggagagag tattatatat
     3541 aatactactt ctaaattttt tcaggaggta agataatggg aagtctaaaa atatatccaa
     3601 gtaaattatc tggagatgta aaaataccac catcaaaaag tattgcgcat agggcagtta
     3661 tttgttcatc tctaagcaat ggaaaaagta gaatttctaa tatagatttt tctgatgata
     3721 taatagctac tataagagca atgacatcat taggagctat tatagaaaag aaagaagata
     3781 ttttagaaat ttcaggaatt ttttcgaagg aaggtatttt aaatagagag aatcagttga
     3841 atcaacctaa attgacaata gattgtaatg aatcaggctc tacattgaga tttttagtac
     3901 ctatatcttt agcatttgat ggtgtaaaaa ggtttatagg tagaggtaat cttgggaaaa
     3961 ggccacttga tacttattat gagatattta acagacagaa tataaaatat agttacaaag
     4021 aaaatcagtt agatttgatt atatcaggta agcttaaacc agatgagttt agagtaaaag
     4081 gaaatataag ttctcagttt atcacaggtc tattatttat attgcctaca ttagaaagtg
     4141 attcaaagat aataattact actgagttag aatctaaagg atatttagat ttaactcttt
     4201 caactatgaa agattttggg atagaaatta taaacaataa ttataaagaa tttattataa
     4261 aaggaagtca aacgtataaa gcgagagatt ataaagtaga aggagattat tctcaaggag
     4321 cattttattt atcagcagat gctataggag aagatattaa tatattggac cttaaagaag
     4381 attctcttca aggagatagt gaagtagttg aaattttatc taggatggga atggaaatat
     4441 taagagaggg taacaaaata aaaggaataa caaatggttt aaatagtact ttgatagatg
     4501 catctcaatg cccagatata atacctgtat tatctgtagt agcagcttta agtagaggga
     4561 aaactacaat aatcaatgct ggtagactta gaattaaaga atgtgataga cttcatgcaa
     4621 taaatattga gttaagtaaa cttggagcga atatagagga gaaagaggat agtttgataa
     4681 tcgaaggagt aagtaagtta aatgggggag tagaggtgtg gagtcacaaa gaccatagaa
     4741 ttgctatgac acttgcaatt gcttcttgta gatgtgataa accaataata ctaaaagatt
     4801 ttgagtgtgt atcaaaatct tatccacatt tttttaaaga ttttgagatg ttaggaggac
     4861 gaattgatga gtggaatatg gggaaataat ttaaaaatat ccatttttgg agaatctcat
     4921 ggaaatgcta taggtataaa tatagatggt cttccttcag gagtggaact tgaccttgag
     4981 aagattgata gagaaatgaa gagaagagca cctggaaaaa attctatatc tacttcaaga
     5041 aatgaaagtg atataccaga aattctaagt ggatatttta atggaagaac tacaggaacg
     5101 ccattatgtg caatcataag aaatagtgat actcgttcaa aagattatgg agaacttaaa
     5161 aatctaatga gaccaggaca tgcagatttc acaggaaatg tgagatactc tggatttaat
     5221 gactacagag gtggaggtca tttttcaggt aggataacag ctccattagt attttgtggg
     5281 gctatatgta aacaaatttt gtcacaaaaa ggaatagagg taggagcaca cataaaaaaa
     5341 attaaaaata tagaagatat gagttttaat tatgtaaata tttcaaaaca acagttatca
     5401 aatttgcaaa ctttagaatt acctttactt gatttatcaa aggaagaaaa aatgaaaaat
     5461 actattatag atgcaaaaaa tcaaagagat tcagttggtg gtataattga gtgtgcagta
     5521 gtaggtgtta atgtagggtt tggaaatcca ttttttgatt ctgttgaatc tactttatca
     5581 catttattgt tttctgttcc tgctgtaaaa ggcgtagagt ttggtttagg ttttgaactt
     5641 gcagatatgt atggttctca aagtaatgat gaaatgtatt atgaaggaaa tcaagttaaa
     5701 agcaaaacaa ataataatgg aggaattatt ggaggtatta cgactggaat gccaataatt
     5761 ttcaaggtag caatcaagcc aactccatca attagtagac aacaaaacac agttaatata
     5821 aaagagaaaa aggacgatat tttatctatt aaaggcagac atgacccttg tatagttcaa
     5881 agggttatac ctgtaataga agcagtaacg gccattggaa tttttgattt gatgaaagga
     5941 cgataataat ggaggattta acgagatata gaaatgagat tgacaatata gataaagagc
     6001 ttattcaatt atttgaaaag agaatgaata cagttcttga aattgccaga cataaaatga
     6061 aaaataacac tactatactt caaaaagata gagaagaaaa agtactaagt agagccgtag
     6121 ataatttaga caataaagcc tattcacaag aaacaataca attttttaat tctataatgg
     6181 aaattagtag aggattacaa aaaaggttga tggataatga tagtgagcag aaaattgaat
     6241 ttaggctaga ctcatcaaag aagaaatttg acttgtcaag tgtaaataaa tataagtctt
     6301 taaaagaaga attaaataaa aaaaatatcc tagtaggttt tcctggaaaa tcaggttcat
     6361 ttacagaaga agcactaaat aaattcttca acaaaaaaac agcaaaaaaa caatttaaag
     6421 aatttgaaga tgttttcata gcacttaaaa ataaaaaaat agactatgga ataattccca
     6481 tagaaaattc atccactggt gctatctctg aaacttatga ccttttaaga aaatacggtt
     6541 tttatatagt tggagaagaa tgcataaaaa tagaccaaaa tttaattggg ataaaagata
     6601 caaaacttaa agatataaaa gaaatatatt ctcacccaca aggtatagga caatgtagtg
     6661 aatttttaaa acaaaatagt gcatggaagt taataccatt taataacaca gcaacaagtg
     6721 ctgaacttgt aaaaaaactt caagataaaa caaaagcagc aattgcaagt aaaaaagctg
     6781 caagtattta tggattagaa ataataagtc cttgtataaa tgatattaca aacaactata
     6841 caagatttgt agtaatcagt aatcaaatac acattgaaga agagtcaaac aaaatgagtg
     6901 tagtgttttc tgttgaacat aaagcaggaa agctttataa agtacttgga tactttgctg
     6961 aaaataatat aaacatgaca aaaatagaat caagacctat gaaaaatacc tcatggagat
     7021 atttctttta tatagatttc gaatgttcta tatacaattc taaagtatac aatttgttag
     7081 aattgataga acttaataca gcgtatttta aatttatggg agtatataaa aataaaacta
     7141 aatagataag ataaattttt atttaattat taggaggtgt aaatatgaat ttttttggtt
     7201 tagtaggtga aaaactctct catagtgttt cccctcaaat ccacaagaga gtattcgaaa
     7261 tattgaatat tgaaagtgcc tataaaaact ttgaaatttc aaaagaagat gtatctaagc
     7321 tagctagtgc tataaaatta ttaggaatac aaggagtaaa tgttactgta ccatataaag
     7381 aacgtattat gaaatatctt gattttatat caccagaggc aaagcgtata ggagcagtta
     7441 atacaatttt actcagagaa gatatgttat atggttataa tacagattac tttggtcttg
     7501 atagtatgtt taaaatggct aatattgatg ttcaaggaaa agtggcagtt atattaggaa
     7561 ctggtggtgc ttctaaagct gctattacat atcttctcga ttctggaata gagaaggttt
     7621 atgtttcaac tagaaaaaaa gatgataaaa aattattaaa tagtaaagct atattggtag
     7681 attatgaaga acttaaatgt ataacaggag atataatatt aaatgctaca ccagtaggta
     7741 tgtaccctaa tgtaggaatt tcaccagtgt caaaatctat tatacaaaat tttgatacat
     7801 tagtagattt aatctacaat cctggagaaa cagagttttt aagaattggt aatagtattg
     7861 gtaaaaaaac ttgtgacggt ttatatatgt tggtagggca agcgataaaa tctcaggaaa
     7921 tttggcaaaa tgctaaaata gataatagta ttttagatgt tatatataat gaattaaaat
     7981 tagaattttt gtaagggaga ttgtcatgac aaataaaaca aaagaaaaat taatattaat
     8041 aggtatgcca ggtagtggaa agactacaat tggaaaactt ttggctagag aatataattg
     8101 tagtttttgt gatatggatg attatatagt taaaatagcc caaaaaagta tagcagagtt
     8161 gttttcagaa ggagaagata tttttagaaa ctatgaaact caagcatgta gggaactttc
     8221 tataagtaat aaagcagtga tatctacagg tggaggtgta ataaaaaagg atgtcaatat
     8281 ggaaatatta aaagaaacag gtataataat ttttattgat agacctatac aaaagatttt
     8341 agaagatata aatattaatt caagaccgtt attaaaaaac ggaaaagata gattgtataa
     8401 tctttataat gagagaataa atctatataa aaaattttca gatatagaga ttttaaatga
     8461 caaatcatta aatactgctg tatataatat tataaatgca gttagtgaaa aatttaaatt
     8521 tgaatttaaa gaaaaataat ctaggaggaa ataaaaataa tgaatattgt tattgtagga
     8581 ttaggagtta taggaggttc ttttgctaaa gcattaaaaa aagctggata tgaaaatgta
     8641 tttggagtag atgtagattt agaaacttta aaaaaagcag aaaaagcaaa aatcataaaa
     8701 aaaggatgta ctacaggtaa agagttattt aaaaaagctg atttaattat actatctata
     8761 tatcctagat tagttgtaga ttttctaaat aataataaaa acttttttaa aaaaggaact
     8821 ataattactg atactacagg aataaaagaa acacttatca atgatgtgtt gcaaattatt
     8881 ccagatgata tagattttat atttggccat ccaatggcag gcagagaaaa aaggggaatt
     8941 gattttgcaa gtgaacaagt ttttaatggc gcaaattaca taattactcc aactggcaga
     9001 aacaacataa ggaatttaga gttagttgaa aatttaattt taaaacttgg atttaaaaga
     9061 gtaaaaaagt taacttcaca aaaacatgat gagattatag cttttacttc tcagttacct
     9121 catgttatgg cagttgctct tataaatagt gatgaagaag atagagatac aggtaaattt
     9181 attggagata gttatagaga tttaactaga attgcaaata tgaatgaaga cttatggagt
     9241 gaactttttc tagggaatag agataattta ctaaaagtta tagaaaactt tgaatcagaa
     9301 gtaaacttaa taaaagaagc tatatttaac aatgataaaa gtaaattgat agagtatttt
     9361 aaaaaatcat caataaggag agaaatttta gaaaaataaa tattgatatt taagctgtat
     9421 atagatgggt ataaatataa gcgatagtgt atttaattgt attaataaat atggattcaa
     9481 tataattaaa tatgctattg atatatttgt acccttaaat attgttatct tttaaatgat
     9541 aatgacattt acctaaatac tctttattaa caatttataa aacttaattg taaaaaatac
     9601 ttattaatag taaaataaag tgaatcaatt atattataat tgctaaagag ggtgaggtgt
     9661 agctatttga aaaaaatata ttaatagtaa ggaatgtatc aatagcattt atagtactat
     9721 tcttttttag tgtatttgca ttcttttatg tagccaatat taatagggtg ttagaatatg
     9781 aaacaaataa cttaatgaca attacaatta ctggatggat tatactttct tttttatttt
     9841 taggtattat aatttacata ttatattcaa aagcaaagag tcaaaaaact atagaaaaag
     9901 tagcatatac ggattttgta acaggttatt ccaactggag aaagtttgaa ttggatgtta
     9961 caaatttatt aaagaaaaca tcgcaaaata ataaatatgc aatggtcata tttgatattg
    10021 ataaatttaa agcaattaat gatatatatg gccataaaaa gggaaattta atattaaaag
    10081 atattgcaga tactttaaat gaattgatta atatcaatga aacttttgct cgtgtaagtg
    10141 ctgataattt taacatatta ttaacatata ataaaaaaga ggatataata aatattatca
    10201 aaaagattat ggtaaataat gaacttgtaa acttatcttt tggaatttat gaaattaaag
    10261 ataaggctct atctgtaagt gtatatagtg acagagcctc acttgcaaaa tcatcaataa
    10321 aaaataatag tgatgtaaac tttgcctttt ttaatgatga attaagagag aaattattac
    10381 ttgaagataa gatagaaaaa gaaatggaat atgctcttga aagtgggcag tttgtaatgt
    10441 atttacaacc aaaatataat attaaattaa ataaattttg tggaagtgaa gctctagtca
    10501 gatggcaata tacagaaaaa gaagtcatat atccaggtga ttttattcct gtatttgaaa
    10561 aaaataggtt tattagaaag attgatatgt acatattaga acaagcttgt aaagaaatcc
    10621 ggtctttatt tgataaagga atttcacctt tacctatatc tgtaaacttt tcaagagttg
    10681 attttttaaa aaaagatttt atttcaaaca tagtaaatgt atgtgataga tataaaatac
    10741 catatggttt aatagaaatt gaaattactg aatctagcat gtttggagat actgatattc
    10801 tatttaatgt gagtaggaat ctacaagata ttggctttat agttgcaatg gatgattttg
    10861 gttcaggata ttcttctgta aatatgctta aaaatatacc attaaatgta ataaaattag
    10921 atagaggatt ttttgttgat gataaagatg ttgacaagag tcaaattgtt ataaaaagca
    10981 tagtttctct cattaagcaa ttaggaataa gggttgttgc tgaaggaatc gaaacaaaaa
    11041 gtcaaataga aatgttaaaa aaagcaaatt gtgatattgt tcaaggatat tatttttcaa
    11101 aaccattacc aattaaagaa tttgaaaaat tagtctataa gatatagtca gcgagtctta
    11161 aatgggaggt ggtgattaag tgataagagc aaaatcgtta aaaaatgtac tgagtattgt
    11221 tttaatattt ttatttacaa taatttcttt ttctgatttt gcatttaaaa ctaatgtcca
    11281 agaagaagaa aatagtaaaa ttaatttaag ggaaatatcc ttacaaagtt ccaatgctat
    11341 aaaaataaaa ctagacagtt ctttgtcctt tattaaaagc atggcaaata tggtttcgta
    11401 ttttaatact atagataact caaaaataat tccattatta gctaatatat caaaagaatc
    11461 tgattttcaa agaatttcta ttgtatctcc tgatggaata agccatacca gcgatggcaa
    11521 aattattgat gtttcaaatt atcaatattt taagaaagct attagtggag aaagtaatat
    11581 atctgaagtt gtaaaatctc caattgatgg aaaggatact tttgttatag ctactcctat
    11641 atttaataag aataacaaaa taattggtat tttatatggt tcttactaca atgataaatt
    11701 agctgaattt atagatataa caagttttaa tagtgaaggg tacatagata tatttgaatc
    11761 tagtggacaa tttgttctaa aatcttcaca tacaaacaag ttgattaagt caagtagtaa
    11821 tatatatgat atatttaaaa aagcttcttt tgatgatggt tatagctata aaaaaatata
    11881 taaagataca aaaaataaca agagtggctt tacgtcatat gaatataaag gagaaaatcg
    11941 ctatgctact tatacaccaa ttggtgtaaa tgattggtat gtaatttctg tgataccaca
    12001 atatactgta aggagaaaca ttgataaaat aaatcaatta gcattttctc ttattttcaa
    12061 ggtattcttg ttatttatta tacttataat atatattatt tataagaata ataaatctca
    12121 gagagatata ctaaaagcta acaaagaaat ctcatctatt acaaatagca ttcctggagg
    12181 agtacaaaaa tgtaccttgg gtgaaacatg caaaatcaat tttttaagtg atggatttat
    12241 agagttgttc ggttatacaa gagaagaaat aaaaacattg tttaataacg atttttatag
    12301 tttaatacat aaagatgatg ttgaaagaat aaaaaaagaa atttctaaac aactttacaa
    12361 aaataaaact attgaattag aatatagaat tataacaaaa agcggtaata cagtatgggt
    12421 tttagataag ggagagctta taacagaacc tgatggaaaa gaatattttt gttgtgtaac
    12481 tgtagatatt actagaacaa aacaattaga acaagagatg gaacaaaaaa atctagaact
    12541 tgaaatgata tcagaaaata ttttaggtgg tatacttata acagaactta atgaagattt
    12601 tactataaaa tacactaact ctggttatct ttcaatgatt ggatatacac gtgaacaatt
    12661 agaaaatgaa cttaaatata aagcatgtga ccttgttttt tatgaggata gaaagaaggc
    12721 tctggaaagt atacaacatc aattaaatac aaaaggaata gctgtatatc aacatagaat
    12781 aagaagaaga gatggtaata ttatctgggt ttctataaaa ggaaggctta ttattgatga
    12841 tgatggtatt gaaaaaggta tttggattct aaatgatata acagatttaa aaaatgcaga
    12901 acaaaaactt aaaataaatg aagaaagatt tagaattgca ttaaaacaaa catcaaacac
    12961 tatatttgag tatgatataa aaaccaagtc acttttaaat actagcaaag agcttgataa
    13021 gtataatatt tataaaatca ctgaaaatgt tccgtattct ttagtagaat caggagttat
    13081 tcataaagat tttagtaatg attttataaa tatgtatgaa aaagtaataa atggagcaaa
    13141 aaaagcaagt tgtgtagtta agatgaaact tccagatgaa aattatatat ggaataaagt
    13201 tactctaact actatttttg atgaagatgg taatcctgta agagcaattg gtatacagga
    13261 agatataagc aaacaaaaag aagcagagat aagatacaat caagaaggga agtatcattc
    13321 agctatattg tcggaagcaa tgataactta tgaaataaat gtatcaaagg atacttttat
    13381 caaaggcaat gaaaattggc aaaaaccatt tggaattaaa ccagacaata actattctca
    13441 aatggcaaaa acgatagcaa atagcattgt acataaggag aataaagaag tatttttaaa
    13501 tacattttta tgtgaaaatt tattagacgc atataaaaat aatataactg aaataaaatt
    13561 agaatatcaa agaatgaatc aagaaaataa aatgatttgg gtcatttgca caatgcactt
    13621 aataaaagat tctattacga atgatataaa agcacttgct tatgttaaag atatcgacaa
    13681 acaaaaaaat gaagaaatta gattaaaatt aaaagctgaa agagatactc ttacaggatt
    13741 atataataaa ggtactacag aaaaattgat taatgatttc ttaaactctg agaaagcaag
    13801 cttaggaatg catgcatttt taattgtcga tatagataat tttaaatctg tgaatgataa
    13861 tttaggtcat gtatttggag ataaagtatt atttgaagtt tctaaacacc taaaacctat
    13921 atttagaaaa gatgatatag taggtcgaat tggtggagac gaatttatag tattcttaaa
    13981 aaatattcaa tcttttgaga atgccaaaaa gaaagcagaa aaaatatgcg atatatttag
    14041 aaattcttat acgaaaaata aaaagacata taaaatatct ggaacagttg gaatttcatt
    14101 tgtaccagaa catggaactg tatttgaaga actttataaa aaagcagaca tagcacttta
    14161 ttatgcaaag agcaaaggaa aagataagta tgcactttat aatgattgca tctaccaaaa
    14221 tgaacttgaa aataatataa gtgacaataa tataaaagta gcaaataaat gtgaaaaatc
    14281 caatccagtg cttaaagaag ttcttgattc tattgataat tatatatata tagtgaatcc
    14341 aaatacttat gaacttcttt ttgtcaataa taaggtgtct cgctcaggtt tagagataaa
    14401 attaggacaa acctgttata aatcactatt aaaatctgat agaccatgta gtaattgtcc
    14461 tcttaacgga ttagaagaaa ataaacattc aaaattcaaa atagagacaa atacatttgg
    14521 tacaaatatc agtatagcca gttggattaa ttgcatagac ggagaatata attgtcttat
    14581 agagtgtata gattcttctt tatacaaaaa taaataaaaa ggactatgaa gatatgttaa
    14641 atggtataac tttgatactt ggtataatat ttacaattta ctatttttat ctcaaaatag
    14701 tttttggagg aatttcattt agtgagattt tcttagttgt aggaattata ctaattatct
    14761 atcaaatatt taagaaaaaa atcaaagaaa agaaggtttt ttatagagtg ttaaaaataa
    14821 taatcagtgt ttttttaata gtatttgtag ttactgagag tctaattatt ttttatccta
    14881 aaaatagttt agagtcaaaa agtgattatc tcttgatttt aggagcttct gtaaaaaaga
    14941 ctataccaag tacaacccta aagggtagat taaacacagc attgaaatat ctaaaagtta
    15001 atgataattg ttatgtagta gtatctggtg gtaaaggaag tggagaaaac ataactgagg
    15061 caaaagctat gaaagattat cttataaaaa acggtataga taagaatagg ataatagaag
    15121 aagataaatc cactaatact tatgagaatt ttaagtattc aaaatttaag atagaagaac
    15181 gtagtgaaag aaaattaagt gatttaaaag taaaaatagt aacaacagat tttcatgttc
    15241 ttaggagcaa aatattagca tatagaaatg gatataaaaa tacgtctttt tatgctagta
    15301 aatcaaaact ttcatttgta cctacttact atacaagaga attttttgca ttatggaaaa
    15361 caatcttttt tgatagataa taaatagaag gactagatat aaattataaa tttatatcta
    15421 gtctttttta tgtagaaagt aagagttaaa aattattttt tcaaagcttc ctgccattaa
    15481 attagaaaat ttagtctcag tattttagaa acattaataa tagtaatata aatcaatata
    15541 aaaataatta caagattata ttaattattc aaattatata taaaattaag aaaaattctt
    15601 ttagtttaca taataaaaat aatgttaatt aaaattatta gttttaggta aaagtaccaa
    15661 attatgcatt taagtagatt ttaagctcga tttgattaga aaggtacaaa ctatcgtagt
    15721 aaaaatttaa acctatttaa aaaccaatat aaaagcttgt tatttttgag aaaaataaac
    15781 tctctaaacc caattagaca aaaataaaat acagaaagtt atatctcaga atgtcttaag
    15841 taattaagtc taaaatttat gatattttgt ttggtgattt gtaatataaa agcctttttt
    15901 aaatttgttc aaaataaaaa agtaaaaaat aacaaaaaaa tcactgacat ttcactgaca
    15961 aaacacagtt ttataattat gtatttattg aaaatacttg attattaaaa aatggcataa
    16021 aataatacat tttatgccat ttgctattat ttgataattt ttaagttata atacaatata
    16081 ctttctttac atatataata aaatattaaa aaaccgatat cgatatttca aacagcaaaa
    16141 atagatgtta gctattatag aaaaaaagtg cacttatagg cttaaaatgt cacataaaag
    16201 aattttaaag aataatatat tatgaacaca aatttttata atctcaaagg agtgataata
    16261 tggcaacagt atcaggacaa atagtttttg atagaaacag aagtgctacc attgattctg
    16321 gagattctgg aatagccaat gtcccagttg tattacaaga catagcaact ggagtcagac
    16381 tcacagttct cacaaatgca tcaggaaatt atgtttttac taacgttcca aatggtaact
    16441 atagaattgt agaagctttt ggaactacag gaggagtacc aacaccaggg aattttgcag
    16501 cagcgatagt aggtccagtt ccaattgcag aaataccacc tattagttct gtgataaatc
    16561 ctcctattgg ttcaacaaac ttagatggta caacacccaa cacactatta ataacagtag
    16621 caggttcaga tataacaaat caaaatatat taaatggtcc tgtgatttat acacctatac
    16681 agaatatatt ggattcttgt gtatctgtat ctaatataaa ccttattata gacgcagata
    16741 atggaacatt cggtttcttc ccaccaggca caccaagtaa tacagggcct gagatagaac
    16801 cttacccaaa tgtgacacca gattttacgt atgttgtacc agacccaact aagtttacac
    16861 ctttagatgg agaatataca gtacaaaata ttatgacaaa tgcaatgagt aatgttatag
    16921 gagcttggtg gcgtattgct gaccatacta caggaaatga aacaggaaga atgatggtgg
    16981 taaatggttt taatcctgga gctgtatttt ttaaatcaac agtacaagtt acacctaaca
    17041 ctaatttttt atttagtact tggattttaa acctttttaa ggtaacagga tatccaccac
    17101 cacagctagg tgtaagaata tttgaccaaa ataataatat actatatcaa gcaacattag
    17161 gagctcaaat tccagttaat acaaatgcac cagagtggaa agaaataggt actgtaatta
    17221 attctcaaaa taataatcaa cttacagtag aattttttag cgagggagaa agtgttattg
    17281 gaaatgacta cgctatagat gacgtttcct taagagaaat tactgttcca acttttactc
    17341 caataaaaac aagtaacaca gaaactgcta acataggcga tatagtcaca tatacagtta
    17401 cactagataa tacttgtaat agccctttaa ctaacgtatt ttttagggac ataattccaa
    17461 acggtttaaa atttatacct ggaactgtaa cggttgatgg aatatctgta ccaggagtag
    17521 accctaatat aggtttccaa cttccagata tcggaggagg aacaaggatt gaagtaactt
    17581 ttaatgttgt agttgagagt attcctaacc caaatcctac taataatata gctactatag
    17641 attactccta tactcctgta gaaggtggta taccaaataa tttttcagta gattctaatc
    17701 cagttcctgt tgaagtaatc tcagcagata ttgaagttac aaaattatct gaaccttcta
    17761 tagtgaatcc aggagaggaa ttaatataca ctataaaagt agtaaataac ggaccattcc
    17821 catctgaaaa tgtggtttta acagatgatg taccagactc tattataaat tcagaatatt
    17881 cattggatgg aggagtaagt tttcaaccat ggaatggaag tttaaatata gggacattag
    17941 aattaggagc gacaagagtt attataatta ggggtattgt aaatccaaca acagtaggaa
    18001 ttataagaaa tacagctgta gtatcttcaa ctacagcaga cccaaatctt aataataata
    18061 catctacaat agagacagaa gtgaatttat tggcagatat tttggtaatg aaaacagcag
    18121 aacctaattc agctgttcca ggtactttat taagatatac tattcaagta gcaaacttag
    18181 gaccagcaaa tgctgagaat gtaatattaa atgatgatat accagatgtt attttaaatc
    18241 cagaatattc actggatgga ggagtaagtt ttcaaccatg gaatggaagt cttaatatag
    18301 gagctttaaa ctctggaatt tcattgacag ttttaataca aggaacagtt agtttaaatt
    18361 cttcagaata tatagtaaat acagcaacag tttcctcaac tacacctgac ccagatttaa
    18421 gcaacaatat atctactatt atcacgccag ttaatccaca ggcaggtatt tctattataa
    18481 aagtggctaa tgaggatgta gctgttccag gagaagagtt tgtatataca attgaaattt
    18541 ttaatgaagg accatctaat gctatgaatg tagttttaac agataatata ccagatgtta
    18601 ttttaaatcc agaatattca ttggatggag gcgcaagttt ccaaccatgg aatggaagtc
    18661 ttaatatagg tacagttgca ccagggcaat taataagaat tatcataagg ggattagtaa
    18721 gttcaactgc tacagggaat attactaata cagcagaagt aagtgcagag gtaccagaac
    18781 cagttactga ttcatcaaca gtaataactc ctgtagttgc atcagcagat atagaagtaa
    18841 taaaaactag caatatggat acagcagttc caggagaaat ttttagttat actataactg
    18901 ttacaaattt aggtccatct gctgctcaga gtatcgtttt gacagatgat ataccagata
    18961 ttattttaaa tccagagtat tcattagatg gaggagtaag ctttcaacca tggaatggaa
    19021 atcttaatat aggcactttg gatacagggg aaataagaag tattataata agaggaacaa
    19081 taaaccaaac atcagtaggc acaattataa atacagcaac agtaagttca actacacctg
    19141 acccaaatcc agataataat acttcaacag tggaaacaga tataagtcca ttagcagata
    19201 tttctgtgat aaaaggcaac gaaccacttg ctatacaagg tgaaagattt atttatggaa
    19261 ttgaaattgt aaatgcagga ccatcttttt cagaaaatat agtcttgaca gacaatatac
    19321 cagcttctat tttaaatcca gagtactcaa tagacaatgg agtgactttc caaccatgga
    19381 ctggaagcct taatataggt actttagatg ctggagagat aagaaatgtc ataataaggg
    19441 gaacagtaag tcaaacagca gtaggaacaa ttataaatac agcaacagta agttcgacta
    19501 cacctgaccc aaatctaaat aacaatactt caacatcaga agcagaggta agttcatcag
    19561 cagatatttc tgtagtaaaa cgaagcaatc agaatgttgt tgtaccagga gatgtactaa
    19621 actatacaat tgaggtgaga aatgcaggac catctactgc tcagaatgta actttaacag
    19681 ataatatatc agcttctatt ttaagtccag aatattcaat agataatgga gtgactttcc
    19741 aaccatggac tggaagtctt agtataggca ctttgtatgc tggagagata agaaatatta
    19801 taataagggg aatagcaagt gaaacagcaa taggtacaat tataaataca gcaacagtaa
    19861 gttcaactac acctgaccca aatccaaata ataacacttc aacatcagaa atagatataa
    19921 gttcatcagc agatatttct ataataaaat tagcaaataa aacagaagca tgtgtaggag
    19981 aacaaataga ttttgtaata gtagtatcaa atgcaggacc agaaaatgct caaaatgtta
    20041 ctttaataga caatgtttca gataagctta agaaaacagt attttcactt gatagaggtg
    20101 taagtttcca accttggact ggaagtatta atataggtat actaccagct ggaactttaa
    20161 gagttattct attaagagga cttataaaat caacatgtct tgataaatta acaaatattg
    20221 cagaagttac ttcaacaaca ccagattcag accttaacaa taatatatca agagtgcaag
    20281 tagaaataaa acaatgttgt tctaacgatt gctgttgtaa ttgttgttgt aataactgtt
    20341 gtaattgttt ttttaatgat tgttgtaatg attgtggtta caaggattgt tgttgtaatt
    20401 gttgtaaaaa tgacttttgg tgttgatttt atacaataat tttgtatatc ttaaattaaa
    20461 ctgaaataaa aaattcatat atgagtaaaa tattttttaa atattaataa gttttctatt
    20521 ttcgatatgg accacattcg taatactagt tattaaaatg a
//
LOCUS       DAMJVM010000062        20509 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.065, whole genome shotgun sequence.
ACCESSION   DAMJVM010000062 DAMJVM010000000
VERSION     DAMJVM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20509
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.065"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            74..748
                     /locus_tag="PC523_002400"
     CDS             74..748
                     /locus_tag="PC523_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2475914.1"
                     /translation="MFKIMVVEDDVSLKNIIAECLTKWGHNVHQIENLENIIEEFKNY
                     KPQLVLLDINLPFYDGFYWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSIDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIN"
     gene            772..1776
                     /locus_tag="PC523_002401"
     CDS             772..1776
                     /locus_tag="PC523_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2475915.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTIAVIIFSPVDVFLIDTILYI
                     LVINVVFLFLYLFISYIKKNKFLDKIKNDTFKININTTELAKCKNEEKIYLNIIKNHQ
                     YQCNNIINKNIKKFEENKEILNMWVHDVKIPIAIIKLTLEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILNLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRSKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIAVDSEYNQYTKFTITFKCDL
                     "
     gene            1887..3119
                     /locus_tag="PC523_002402"
     CDS             1887..3119
                     /locus_tag="PC523_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDF2475916.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRSSSFGSSGFNSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTKLQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENRDAIASINAQITTLNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVSNSSFKIPASCVLKDKKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     NGDTTPNKKVEEVNMDVN"
     gene            3127..3801
                     /locus_tag="PC523_002403"
     CDS             3127..3801
                     /locus_tag="PC523_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2475917.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKSLKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            3804..5036
                     /locus_tag="PC523_002404"
     CDS             3804..5036
                     /locus_tag="PC523_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2475918.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLIPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVISKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIIATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(5321..6586)
                     /locus_tag="PC523_002405"
     CDS             complement(5321..6586)
                     /locus_tag="PC523_002405"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDF2475919.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMVKAKELDVPLSFHEEDPSLVGNPGVNAGKIASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSREEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSTNPAKLYNLNTGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKINYTICNGKIVYNA"
     gene            complement(6639..7787)
                     /locus_tag="PC523_002406"
     CDS             complement(6639..7787)
                     /locus_tag="PC523_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDF2475920.1"
                     /translation="MKSVSVRSCKTYNYEEVRLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKVHSEKAHVLKYMDIIKPVVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLIPNRVATIRGAIKRGYIREDFSDIEILG
                     EDINDLIIKNYKVPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPAATIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(7878..8423)
                     /locus_tag="PC523_002407"
     CDS             complement(7878..8423)
                     /locus_tag="PC523_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HDF2475921.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(8646..9590)
                     /locus_tag="PC523_002408"
     CDS             complement(8646..9590)
                     /locus_tag="PC523_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2475922.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVIVQAFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            9976..10857
                     /locus_tag="PC523_002409"
     CDS             9976..10857
                     /locus_tag="PC523_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HDF2475923.1"
                     /translation="MVKKRKKKTVALTIIVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAIKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPGRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(10918..12126)
                     /locus_tag="PC523_002410"
     CDS             complement(10918..12126)
                     /locus_tag="PC523_002410"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDF2475924.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKDGSVNPRDIKMNLAREIVTLYHGEESAREAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVVNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(12199..12451)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(12602..13591)
                     /locus_tag="PC523_002411"
     CDS             complement(12602..13591)
                     /locus_tag="PC523_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HDF2475925.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIISVSTGFGYAIYLDF
                     IVPIFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWATQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKLLIFKNFRTLNQFLCCSKKVFYICV
                     SYLTIFEVVKLLNVKVDFVYLKTVFISIAYLCVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RISFLIIILLLFFMFDVTVISLIIINAFLNKKINIRLSQNNIVNKQINLLIEK"
     gene            13743..14183
                     /locus_tag="PC523_002412"
     CDS             13743..14183
                     /locus_tag="PC523_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2475926.1"
                     /translation="MFKIKHFNDLNLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKN
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSRHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            14510..14737
                     /locus_tag="PC523_002413"
     CDS             14510..14737
                     /locus_tag="PC523_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2475927.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            14763..16883
                     /gene="feoB"
                     /locus_tag="PC523_002414"
     CDS             14763..16883
                     /gene="feoB"
                     /locus_tag="PC523_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2475928.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKHKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEAIKKGITIDKKLLEDSLC
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(16996..17814)
                     /locus_tag="PC523_002415"
     CDS             complement(16996..17814)
                     /locus_tag="PC523_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2475929.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKSEEYLVLSSDLSNMSNGDFMMELIDFTNYCK
                     AKNLYQGYELGVIVSNENLKNGDYLSISSFYLKIDKKIKDKRLYIKPEGMYACIKHIG
                     KYENSYKSYEKLKKYIYENEYKIIGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            complement(18268..20361)
                     /locus_tag="PC523_002416"
     CDS             complement(18268..20361)
                     /locus_tag="PC523_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2475930.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIVFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVVELLKNDKKGVLIKWYEKCNKLNCVFENYDSLITWQD
                     GTVVHMHQSIDITNSTSLNKQIKPNEFHKISNTEEEKGVFNVSIDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVENPLIYWEKIVHPEDWKRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGKPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLNIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGIIVDNVEEKEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKYASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTQSHKIIGVEVLLRWTNDKYKAISPLEFIPILEEHDMIN
                     TVGAWVLRMALRTFKGLIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDEKFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDVVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQMKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT         7217 a         3134 c         2603 g         7555 t
ORIGIN      
        1 aggtagaaat attttagttt atcaagtaaa atatagttat gttaatataa ctattttaaa
       61 ttgggaggta agaatgttta aaataatggt agttgaagat gatgtctcat tgaaaaatat
      121 catagcagaa tgtcttacta aatggggaca taatgttcat caaatagaaa atcttgaaaa
      181 tataatagaa gagttcaaaa actataaacc acagttagtt ttattggaca taaaccttcc
      241 attttatgat ggtttttatt ggtgtaatga aataagaaaa atttcaaaag ttcctataat
      301 ctttatttct tcaagaaatt ctaatatgga tgtaataatg ggtgtaaatc taggtgcaga
      361 tgattatata caaaaacctt tttctataga tgtcttagtg gctaaagtta atgccctttt
      421 aagaagaact tataattttg ttgataataa ctcaaatcag attattcaca atggtgttac
      481 tttagattta tcaactgcaa caataaacta tgaagataat accattgagc taacaaaaaa
      541 tgaaatcaaa attctccatg agcttatgaa atataaaggt cagatagtaa gtagaaataa
      601 actcatgaaa aagttatggg ataatgattg gtttgtagat gataatacat taactgtcaa
      661 cgtaaataga atacgctcaa aattaaatga aattggtcta gaagatttta tagaaacaaa
      721 aagaggattg ggttatataa tcaattaaaa cgattctaag taggtgaatt tatgagcttt
      781 aaagattttc tcaaagataa gttaggtttt ttagtataca attttacttt cttaattttt
      841 acaatagcag ttattatatt ttctcctgta gatgtttttc tgattgatac tattttatat
      901 atattagtta taaacgttgt gtttttattt ctctatctat ttattagcta catcaagaag
      961 aataaatttt tagataagat aaaaaatgat acattcaaaa ttaatataaa tactacagaa
     1021 ttagctaaat gtaaaaatga agaaaaaata tatttgaata ttattaaaaa tcatcaatat
     1081 caatgtaata acataataaa caagaatatt aagaagtttg aagaaaacaa agagatttta
     1141 aatatgtggg tacatgatgt aaaaattcct atagctataa taaaactcac cctcgaacaa
     1201 aatgaaaatt taattgatga aaaaatttca gatgacatag atagtgaaat atttagaatt
     1261 gaaaactcag ttgaaaaaat tttatattta tcgagacttg aagattttca taaggatttt
     1321 ttaattcaag aggtaaatat tgaaaaaata attagaaata ttataaaaaa atactcaaaa
     1381 tattttattt caaaaaaaat tattttaaac ttaaataatt tgaattttaa agttttatct
     1441 gacgaaaaat ggctctactt catattcgag caattattga gtaattccct aaaatactcc
     1501 tcattaggag ataatatatc tatctattgc aaaagaagca aaaattattt acaattaagg
     1561 gtggaagata ctggctgtgg tataaaaaag gaagatttaa atcgaatatt caataaggga
     1621 tttacaggta ataatggaag aaacaattct aaatctacag gacttgggct ttatttagta
     1681 aaagaacttt gtgataagct tggccataaa attgctgtag attcagagta caaccaatat
     1741 acaaaattta ctattacatt caaatgtgat ttatagtatc ttattattga tagtttattt
     1801 tcattaactt atattcaact tcttatagta attctatatg tacatcccag tatataataa
     1861 gtagttttta taaaaaggag gaagacatgt cttttctaaa aaaatttaaa aataaaagat
     1921 ttagaaataa cagttttaaa agtaacaact ttagaagtag cagttttgga agtagtggct
     1981 tcaattctaa gaataagggt tttaaaaaca atccttttaa aaataagaga ttcaaaatat
     2041 atgcaggaat agctgtaatc atacttctaa ttctaggagt tcttgcttat gttgattcaa
     2101 aaaacactaa attacaagct aatgaaaact ttatcgaaac atatactatt cctgaaaatg
     2161 aaaaaatatt tataaatggg atggtcgttc ctaagcaaac taaggatttc aatatatctg
     2221 gagattatga gttaagtgat gttaatgtta caaatggtca aaaagtaaat caaggagacc
     2281 ttctttttac tgctaaaaat ccaactataa tagcagaaat agatagttta aaatcacaac
     2341 ttagtcaata caaaaaacaa aaaatatcac tttctgatat tgctgaaaat agggatgcta
     2401 ttgcctctat aaatgcacag ataactactt taaactctca gatagcttct ctggataaaa
     2461 aagcgtatga cagagttact gcaccttttg atggtacagt atacttgaat gaccaaactg
     2521 gaaacccaga ccaaccagtc tcatttatga ctatccaagg tctagagttt tatatgaaag
     2581 gacaagcaag tgaacaagat ttacctaaaa tgaagattga ccaaatggtc aatatattag
     2641 ttttttcaac tgaccaaaag cttactggta gaataagttt tatatctgat aaaccatcta
     2701 ctccaaacac tgaaatggga gctcagcaga atacactgtc ttattatgat ataaacatag
     2761 catttgataa tcaagaagga ttagtaaatg gattccatct tcaagcatca cttgaagtat
     2821 ctaattcaag ctttaaaata ccagcatctt gtgtgcttaa ggacaaaaaa cactcatatg
     2881 tatttaagga tttagatggt atattgaaaa agcaaattgt agatgtagca agtcaaaatg
     2941 atgattttgc tgtagttaga ggcggtcttg aacaaggcga tataataata aaacacccta
     3001 ctaaagaaat gaaagaagga gacccagttc aaggtgatgg cgtaactgct ggttctaata
     3061 ataatggaga tactactcca aacaaaaaag tagaagaagt taatatggat gtgaattaat
     3121 ttacatatgt taattaaatt agaaaatatt caaaaatatt acaaagtagg gaaagatgaa
     3181 ttacatgttt taaaatcctt aaatttagaa attgaatctg gagaatttgt catgataatg
     3241 ggtaaatctg gtagtggtaa aactacttta cttaatatct taggttttct agatgtattt
     3301 gatgaaggaa gatatatatt tgatggaaca gatgtaacta atttgagtga aaatgaacgt
     3361 tctgttttta gaaatattaa tattggattt gttttccaac aatttaattt aatagaaact
     3421 ctaaatgtat atcaaaatgt agagttgcct ctaatttaca ataaatcgct aaaaaaatct
     3481 aatagagaag aaattgttaa agataaatta agttctgttg gacttttgga taagcttaaa
     3541 caaaaaccgc ttcaattatc tggtggtcaa caacagcgtg tagccattgc acgttgctta
     3601 gcaaatgacc ctcaaattat ctttgcagat gaaccgactg gggcattaga tagtgaaact
     3661 agcagagaaa ttatggagct tttaacaagg ctcaacaaac aaggtaagac tataataatg
     3721 gttacacatg atcaagattt aactaagtat gcaactaagg ttatacgact taaagatggt
     3781 gtgtttactt cggaggtgta atcatgagtt tattaaaaaa ttctttggcc aatttaaaag
     3841 gtcacaagct tcgtgtattt gtagctttac tctggattat aataggaata acttcagtga
     3901 tacttgttag ttctataggt aatggatttc aaaaagaaat taaaaaatct gtaaacaatg
     3961 taaaccctaa taaaactact atttcatttg agtcagctga taacacaggt cttactgatg
     4021 atatgagtat ttttttaaaa ccatttaacg cgaaagactt agaagagtta tcatttgttg
     4081 aaggagtaga aagaatagct ccttcaagag atgggtttaa ccttgattca gtttactctt
     4141 cacaagcatc ttttgataaa aaaactacgt atgttgatgt tggaccagta aaaaaagatt
     4201 ctaaaataaa tttaatttgt ggtagagatt tttctttaga tgatgaaaaa agaaaagtaa
     4261 ttttacttac attacaaagt acaagtgaaa tttttgaaaa tccagaagat gccttaggtc
     4321 atggtatcaa cataaatgga actatatttg agataatagg tgtattggat gattctcagc
     4381 aaaaccaagc tggaggattc tttggaggtt accaagatat gcaatttact acttccctta
     4441 tacctaaaaa agcttttgac actttaatga gtcaaaattc ttattcaaat gaaatttacc
     4501 aattagactt agtttcctct aaaggctata atgtaaatga agttgcaaac aatgttatat
     4561 ctaagttata tgaaatgcac cctggtatca atggttctta tactacacca gacccaactg
     4621 aacagacagc ttatcttgaa agcattaatt ccaatgtaaa caaatatgta tctataataa
     4681 ctgtagttgc aatgtttgtt ggtggtattg gtgtaatgaa tataatgtat gtatctgtta
     4741 tggaaagaca aagagaaatt ggtataagac gtgctattgg tgcaaagcct aggtctatat
     4801 tatttcaatt tttagttgaa gctgttttta taacagtatg tggtggaatt ttaggaacta
     4861 tagttggatt tgctgctaca aactatgttt ctaaatatat aggctttgaa gcaataccta
     4921 gcttaaatag cttgttttat gcaattatag ctactatatt gactggagtt gtctttggtc
     4981 ttataccagc ctttaaagcc tctaaactag accctataaa ggcaatttat aagtagaaat
     5041 tgtataaaga ttttcgattt ttaaattagt ttatataaaa catacttaaa tatataacgc
     5101 actattatat atgatagatt aataatttaa tctttccaaa tataatagtg cgttctttca
     5161 taaaagggat tctcttcctt ctatatctaa attaccaata tgtcataccc taactgttca
     5221 agctaatatt tccaatttgg attaatcaat ctccttttcc taatatacaa tcttttgctc
     5281 tcaacaattg tatcacccac cttaaaaaaa taatcttaaa ttaggcgtta tatacaatct
     5341 tcccattaca tattgtataa ttaatcttgc catacaaaga tttaccttta aacggtgaat
     5401 tatcagcttt agaaacaaaa ctttcaactg tccattcttc ttcagggttg aatataacaa
     5461 tgtccgctac agccccttct tctataaacc cagtatttaa attgtacaat tttgcagggt
     5521 ttgtagacat tttttctatt aactgcatca tactaagatg atttttatgc actaaatttg
     5581 ttattccaag agctaaggaa gtctcaagcc caataattcc acttggagct tttataaatt
     5641 ctctatcttt ttcttctcta ctatgaggcg catggtctgt tgctattatt tcaatagtac
     5701 catcatttaa tccctctata attttatctc tatcccattt acttcttagg ggtggattca
     5761 tttttgcatt tgttccaaac tccaatacat cttcctctgt caatgtaaaa tgatgtggtg
     5821 aagcttctgc aactacattt gcacctaagt attttgcaaa tctaaccata tcaacagata
     5881 cacctgaact tatatgttgt atatcaacct tagcaccagt ctttagtgcc aacatacaat
     5941 ctctagctac cataacatct tctgctacac ttgatgcacc ttttaatcct agttctgaag
     6001 ctatctttcc tgcatttaca cctggatttc caactaaaga agggtcttct tcatgaaaac
     6061 taagtggtac atctaactct tttgccttaa ccattgcctc cataattaga ttactatcca
     6121 ttaaaggtaa tccatcatca gtaaaccctg gtacaccagc ttttttaaga gcttccatat
     6181 caactaactc tttcccttca aaacctttag ttatagctgc tgcttgtaaa acatttatac
     6241 atgcattttt tgatttttct ttaacataat taaaagtatc ctcattatct acaataggat
     6301 tagtgtttgc catacatata acagttgtaa atccacctct agcagcagat ttagctccac
     6361 tttctatatc ttctttatat gtaaacccag ggtctctaaa gtgtacatgt acatccacta
     6421 aacctggagc aactacatta ccactagcat ctattatttt ttcatattca tcactttcat
     6481 caaatttacc tattttataa attttatttt cttttataat taagtcaata ttctcgtctc
     6541 tttgactttt agggtctatt actctaccat ttttaattaa aatcatatta tttctccttt
     6601 atatttttaa tttatataga ctcatataat attatacatt attttacata cttaaataca
     6661 aaattacgtt tgacatcaat agccttttta ggacaaagtt catggcaaca gtaacatctt
     6721 atacatttgc ttaagtctat aatagcaccc ttagtcccta tttctatagt agctgcagga
     6781 caagcttcct tgcattttcc acattttata caatcttgaa atctaactac aggttttggt
     6841 gttataagct ttgaaatagg ctcatttaaa aattttggta gctttacgga aaacaatctt
     6901 aaatctttac ttacatttgg aaccttgtaa ttttttataa ttaaatcgtt tatatcttca
     6961 cctagaattt caatatctga aaaatcttct cgtatataac ctctcttaat agcacctcta
     7021 attgtagcaa ctctgttggg aattaaactt ataatcttac acgcaacaac gtctatagca
     7081 tatggatttt gtgatgctaa taaaactcct atttctctag tagtgccagc tgagggtcca
     7141 tcaccttcca tacctactat accatccata attgtcaatg tcggttttat ataatcacaa
     7201 atatctaaaa gtacattttc acaaaacaac tcttctgtag gaaatctata atgtatttct
     7261 gcttttttta atccagctat acatccatat aagttttttg taccaccagt aaaagttgcc
     7321 attatatgag tctttaattt acaaagattt attatgtaat ctgaatctac tactggttta
     7381 ataatatcca tatattttag aacatgtgct ttttcactat gtaccttaac atctgaaaca
     7441 tcataattta acttaatgcc caattcattt gctacttctt caattccaga tgccttataa
     7501 atactcttaa gagaactttg attatatgga ccaccagggc tatctgctat tgtaatatca
     7561 caattcaact ctaaaagttt ttcacataca acttttacaa ccataggatg tgtagttgta
     7621 gcttcttcag gctttttttt catcaataaa tttggtttta taagtacttt actccctttt
     7681 ttaacaaact tgtctatacc accaatatcc tctaaatttt tttcaataga taaccttact
     7741 tcttcataat tgtaagtttt acaactccta acagatactg acttcatatt atctccatcc
     7801 ttctatcaca acagtttatt tgtataattt aaaaaaggag aaaccaaagt ttctcctttt
     7861 aatctatgtt ttaattatta ttttccaaaa tatctgttta ataatcctaa gaatgcttta
     7921 ccatgtctag cttcgtcttt acacatttca tgtactgtat catgtatagc atctaagcct
     7981 aactctttag ctctcttagc taattttaat tttccatcag ttgcaccata ttcagcatca
     8041 actctaactc ttaagttttc ttttgtatct gcaactacaa cttctccaag aagttctgca
     8101 aatttagcag catgttcagc ttcttcaaaa gctattctct tataagcttc tgctacttct
     8161 ggataacctt ctctatcagc ttgtctactc attgctaaat acattcctac ttctgtacat
     8221 tcaccagtaa agttcgctct taatccttca attatttctg catctacacc ttgagctact
     8281 cctattctgt gttcatcagc ccaaaccatt tcgcctttca tttcttcaaa tttatcagct
     8341 ccaactttac atactggaca ttgtgctggt gcagcatctc cttcatgtat atatccacat
     8401 actgtacaaa caaatttttt cataattaac ttcctcccaa ttttatataa ttaaattttt
     8461 atttttaagt ttaataactt gctacaagaa tatatatacc ccagactttg tagcttaaac
     8521 actaaaaata aaatttattt cctaattaat tttatcactt tgaaacaaca gtatcaacca
     8581 agttttgatg tcaaatttct acataataca taatttattt aattaattag gaaataattg
     8641 taatattaaa tactttccaa tgaagactct tctgaatctt taagcttcac ttttttgctc
     8701 tttaaaaaag ataatttggt ttctgcagtt atgattgcaa caaatatcaa gatactacca
     8761 ataaccaatt ttgctgtaat aagttccttt agaattatta tagaaaatat tgtaccaaat
     8821 acagcttctg tggataatat tatagcagtt ctagttccat caactctttt ttgacatatc
     8881 gtttgaaata aaaatccaat tgtagtacta aatacagcta ggtacatagt tcccatatag
     8941 ccagataact cagcctccat ctttaattga cctgcaaatg cttgaactat cacagacatt
     9001 aaaaatgcta ctgtaaattg aatggctgtc aatgccattg gattattatc ttttgcaaac
     9061 tcactcgtaa agaatatatg aaatgcaaat ccaaatgaac atataagtgt tagaaaatct
     9121 ccaaaattaa tagagaaatc agcttctaaa gaaagtatcc caattcctat aagagctacc
     9181 aagctactta ttatccctat cttatcaagt tttcttctat aaagtataaa tcctatgaat
     9241 ggaactatta caacattagc tgctgttata aacgcattct tagatggtgt agtgtacata
     9301 agtccaatag tttgtacggt gaaggctaga aataaaaata ttcctaaaat ccctcctgct
     9361 tttaataatt ttttacccat attggcacga atctgtttaa aaaacagtaa attcataatt
     9421 atagctgcca agaaaaatct taaagttata atttgtagag gtgtaagccc accgtctaat
     9481 gcaaattggg ttgctacaaa gccacttccc catataattg ctataaatat tagtcctatt
     9541 tctccaaagt attttttcaa taaagccccc tcctttttat ttttttgcaa aattataaac
     9601 cccctaaaca gcaagagagt ttttctataa gcattactat aaattaatat agtaaatttt
     9661 ttcagtattt taaatctaaa tttacatata ttttgagctc aaatttatat ccaccattta
     9721 acagtaatat attaatcatt ttttgtaaac acctttttga taaatttttt tgttttttat
     9781 atttttttac ctatactaaa tttttttata gataaatttg atatatgtga tactttaata
     9841 ttatgcaaat aatgtcatat aagttacact ttagttatct tagttacaac tttgtcggtt
     9901 tactttttct actttcttcg atataattat aatatatttt tgatacaatt cgacaaaagg
     9961 gggaatgctg taattatggt aaaaaaaaga aaaaagaaaa ctgttgcact tactataatt
    10021 gttgtaatag ttattggtat tttttctatg gcatgggcgg acatgtctag aaaaaaagaa
    10081 atgaaagaaa ctcaagaaag acttaaagct gaaagattag aaaaagaaag aattgaaaaa
    10141 gaaaaaaatg aacctattat tggtgcaaaa aaagaagcta taaaagaata tggatatgat
    10201 gcaaaaatag tttgggacaa acttaacaaa tacgactatt caaacaacgg agaaaagata
    10261 gtctttctta catttgatga tggaccatct actactaata ctccacaagt tctagatatc
    10321 ttaaaaagac atggtgttag aggaactttc tttattaaag gtgattctct tgaaagaaaa
    10381 ggcgctaatg aaattttaaa aagaacattt gacgagggaa atgcaatagc ccatcactct
    10441 tatactcatg actataaaaa actatatcct ggcagaagtc taaatctcga tacattcgta
    10501 aatgaactta acaaaactga tgaagctatg aaaaaagtct taggaaagaa tttttcctca
    10561 aatgtggtta gatgcccagg tggttatatg tcatggaaaa atatggaacc cctaggaaac
    10621 tacttaaaag agaaaaacat ggcatcaatt gattggaatg ctttaaatgc tgatgcagaa
    10681 ggtaaaaaga aaaatgcaca agaattattt gaacatgcaa agaaatcttc tgaaggcaaa
    10741 gaaatggttg ttcttttaat gcatgatact tatggaaaac aagaaacagt aaatgcattg
    10801 gaccaaatta taacgtattt taaagacaat ggttatcaat ttaaaatttt aatatagtaa
    10861 aaaagcaagt ttattgagta tacattttat actcaataaa cttgctttta ctttactcta
    10921 ttttactagc tctattttta caaatttttt ctttcctatt tgaacaacta attcactttc
    10981 ttttacaata gtgcttaagt cctctacttt ttcaccattt acctttacgc caccttgaga
    11041 agctaatctt ctaacttcac tcttactctt aacaatttca ttaacaacta gtacttcgat
    11101 taagtcaaat ccatcttcct taacttgaat tgtttgtata tcttcaggta tttgaccttt
    11161 ttggaataca gatttaaatc tttcttcagc ttctctagca gattcttctc catgatataa
    11221 tgttactatt tctcttgcta agttcatctt tatatctctt ggatttacag aaccatcttt
    11281 taattgagat tctattttgt taacttcatc aggatgaaca tctgttacta aattgtagta
    11341 tttaattata agttcatcag gaatttccat agatttttgg tacattattc ccgcttcttc
    11401 atctattcca atatagttac ctaaactctt actcattttt tcttttccat ctaatccttc
    11461 tattaaaggc atcattatta ctatttgaga ctccatacca aattctcttt gtaacgatct
    11521 tcccattaat aagttgaatc tttggtcagt tccaccaagt tctatatccg cttctaatgc
    11581 tatagaatca tatgcttgca ttaatggata gaaaaactca tgtactgata ttggcatttg
    11641 tccttcatat ctctttttaa aatcttctct ttctaacatt ctcgcaacag taatagttgc
    11701 tgctaatttt attacatctt caaaatttaa cttagctaac cactcactat taaatcttac
    11761 aatagttttt tctttatcta aaactttgaa aatttgttct tcataagttt ttgcatttgc
    11821 aagaacttgc tctgttgtta aagcttttct agctttagac tttccagttg gatctccaat
    11881 ttttcctgta aagtctccaa ttattattac tatttgatga cctaaatctt gtaattgttt
    11941 catctttcta agaactactg tatgccctaa atgtatatct ggagcacttg ggtctagacc
    12001 caattttact atcataggct ttccttcttt ttctgattta ataagttttt cccttaactc
    12061 tttctcatct attaaatcat ctacaccttt cataattatt cgcatttgct cgtctatcga
    12121 tttcattttt cattcctcct aaaatattta tataatctat aatttttatt tttataataa
    12181 aaaaagcttt catcctatta aaaggacgaa agcattgctt acgtgttacc accttagttc
    12241 atcaatatct cacgatatta atctcttaga gtataatggc atatactcgt gtactataac
    12301 gtgtactact ccgatatctc ttaatggttg ttatctttca gagatagtgc tctgagatgt
    12361 attcaaacta aatcaattta ttccatttca ccaaactgga actctctgtt aaataaaatc
    12421 tagctctact tcttctcttc atagcattta tatattcaga ttatttcatc aattacaaga
    12481 aatttttctt aacttactca taatatatta ttatttttct attttgtcaa cctttttatt
    12541 attatataca tgttttttta cattatacta cttttttata ttttttacca atacctaaag
    12601 tttatttttc aatcaaaaga tttatctgtt tattgactat attgttctga ctcaatctta
    12661 tatttatttt cttatttaaa aatgcattta taattattaa actaatgact gttacatcaa
    12721 acataaaaaa caacaataaa attattatta aaaaagaaat tctagcatat aataattttc
    12781 taaacttagc tattataaaa ttttgtaatg acatacatga gtctctaata ataaaataga
    12841 cacataaata cgctattgat atgaagactg tttttaaata aacaaaatca actttaacgt
    12901 tcaacagctt tacaacttca aaaatggtta gataagaaac acatatgtaa aaaactttct
    12961 ttgaacaaca taaaaattga tttaaagttc taaaattctt aaatattaaa agctttttta
    13021 aagaattaga atctaaaata tgcaatttat tccctaaaat caaacttaat aaatatatgc
    13081 aaaacaccat accaaaagga aacaatgcta tcattaaata ataaataaaa tttgtatacc
    13141 cattaaaata aatccatttg ctatatcctt gaaattcttt cataaaacta taacctatta
    13201 atgttgaagc atatatatca ataaaaacca ttacaataac tccaaacact gatccatata
    13261 ctaaatcatc cataaccatt gtaggcttat ttattagata gccacacatt ataccaagta
    13321 atacactctg agttgcccaa atagcagttc ctaaatttcc aagtaccaga gaaattatta
    13381 aaagtgaagt tattccagat aaaattgtat actttaaatc acatttcaag caaattatac
    13441 aaaaaaatat tggcactata aaatctaaat aaatagcata tccaaatcca gtactcacag
    13501 atataattgt tactactatg aataaagatg acaataaagc tgcttcagtt aattttttag
    13561 tagtattcaa aacgctacct ccataaatca taaattttct aactaaagtt ttgtttttaa
    13621 tatagtatat cactttttaa aaatattttt aataaatata aattttaata aaggtattta
    13681 tgttaaaaat gatataatgt atcttaatat tcagtttttt aagatactag gaggtaatta
    13741 ttatgtttaa aattaaacac tttaatgatt taaatttaga tgaattttat gaaatagcaa
    13801 aatcaagata tgaagtattt gcttgtgaac agaagatatt ttcgttaaac gattatgatg
    13861 atatagataa aaactcttat catatattct taaaggaaaa tggattgatt tgtgcttatg
    13921 ctagaataat tcctaaagaa tacagctctt ataatgatgt ttccattggt agagttttag
    13981 ttctttctcg tcatagaaga aaaggtttag caaaacaaat gatggattgt gctatagatt
    14041 ttataaaagt taacttacac gaaaacaata taaccttatc agcacaaact tatataaaga
    14101 acctttactt atcttgtggt tttaaagaaa tctcagaagt atacgacgaa gcaggtattg
    14161 aacatattaa aatgagatta tagtgttgac aagctaatat tgttaatata taatttaatt
    14221 ataaattaaa tattgaattg atatttaggt atttgaaaat accttacata gagtcaggag
    14281 gcttttgctt tctggctttt ttattatcaa aattcaataa ttatatggat tcaaagtgtt
    14341 aatttttatt ctatattcta ctttccccaa aagtaagaat tatacccaac tttgtatgat
    14401 aggcatcttg cctatctttt ttttgtaaaa gtaacaaggt gataatataa tgcatagttt
    14461 agaataagaa gattagccca ggctaaattc caagcatgga ggtgctacta tgaaaaacct
    14521 taacgatatt caagtgaaaa aaactgtcaa agttgaagat atattatcta gtggtaattt
    14581 aagagaaaga atgttagcgc ttggtcttac tagaggagct gttatagatg tagttagaaa
    14641 aggtccaaaa aataatttaa ctgtctataa aatcagaggt agtaagatag ctctacgaca
    14701 agaagaaagt agcttaatat tagtctctga catttaaaaa tttctagcag gaggatatta
    14761 atatggggtt aacacataac tcaactaaaa tgagttcttt aaaagatatg tttgatatag
    14821 acaacaaaga agaccagttt gtaattgcat tggctggtaa tccaaataca ggtaaaagta
    14881 ctgtatttaa tcatcttaca ggacttagac agcatactgg aaactggcca ggaaaaactg
    14941 tagctacagc tcgtggtaat tttaaacata aaaataccga atatgcatta atagatttac
    15001 caggaacata ttctttattt gccttatcac aggaagagat tgtagctcgc gattttatat
    15061 gttttggtaa tccagatgca gtaatagtag tatgtgatgc tacatgtctt gaaagaaatt
    15121 taaatttagt atttcaagtg atggaactta cagataaagt tattttatgc attaatttaa
    15181 tagatgaagc tataaaaaaa ggcattacta ttgataaaaa acttctagag gattctcttt
    15241 gcattccagt tattctaact gctgcaagaa acgggtctgg tatggatgag ctattggata
    15301 ctttaaatga tgtatctttt gacaaataca aattaaataa taagcctgtt agatacaatg
    15361 aaaatataga gaatgtggta aaatcaattc aaccagaatt ggataatatt ataccgggta
    15421 taaattctag atggttggga cttagactta ttgatggtga tgaaagtata tttgaatcta
    15481 tgagtaatta tatagacaaa gattccatag atgctattaa tgaagtaaag aaaaagattc
    15541 cagataatat aaataaacag aaaataagag atgagtttac caaaatcaac tatgattatg
    15601 ctaaaaaact gagtgatgag tgttgttcta atgttgctaa aaaatcaact gatagagaag
    15661 aaaaagtaga taaaatatta acatctaaaa tatttggact tccaataatg ctcttattat
    15721 taggaaccat attgtggatt accatagaag gtgctaacta tccatccact ttattgtcta
    15781 atttgttact cggatttgag ccaagtattt cgggtatatt aaatagtatt aattgtccat
    15841 catggttaaa tgatatgttg gttttaggat tatacagaac attagcatgg gtaatttctg
    15901 taatgctacc tcctatggca atatttttcc cattatttac cttactcgag gattttggat
    15961 atttgccaag agttgcattt aacttagacc acctatttaa aaaagcttgt gcacatggaa
    16021 aacagtgctt gactatgtgt atgggatttg gatgtaatgc agctggagtt attggttgta
    16081 ggataattga ctctccaagg gaaagactta tagctatact tacaaacaac tttgttcctt
    16141 gtaatggtcg ttttcctact ttgatagcta tatctacaat attttttagc tcagttataa
    16201 ccaactcttt cgtatcaagc gtagcaactg cactttgcat aactttactg ataattctag
    16261 gtgttataat aacattatta gtttcttata ctctttctaa gacactatta aaaggagttc
    16321 catctacgtt tacattagaa cttccacctt atagagttcc tcaaatagga agaactttat
    16381 atacttctat aatagataga acaatatttg ttttaggaag agctgtaatg gtagccatac
    16441 ctgctggtgt cataacttgg atatttgcaa atatatatat aggagattta agcatattat
    16501 cacatgttgc aaacttttta gaccctttag ctaagttaat tggtcttgat ggttttatat
    16561 tacttgcttt tattcttgga ttcccagcaa atgagatagt tgtacctata cttttaatgg
    16621 cttacctagc aactggttct atgatagaat tagatagctt tagtgcttta ggtcaagttc
    16681 ttagagaaca tggatggact tatttgacag ctttaaatgt tatgctgttc agtttactac
    16741 attggccttg tgcaacaaca cttttaacaa taaaaaagga aactggtagt ttaaaatgga
    16801 ctgccttagg attcttaatg cctactatac ttgcttttgt tgtttgtttt ttaacaacta
    16861 cagtttacaa tttatttata taattaacat taactgacat tagacatata ttagaatact
    16921 ttattataaa gcatagagat atattcaatg ataagtaaaa ttacttagta tttggatata
    16981 tctctatttt aactcttatc tggacacttg gattgaaatt tcagtcatat aattatcttc
    17041 attactttca caaaaaaagt caagtatact ctcttcatat gaatttccaa taattttata
    17101 ttcattttca tatatatatt ttttcaattt ttcataagat ttatatgaat tctcatactt
    17161 tccaatatgc tttatgcaag catacattcc ttctggcttt atatataacc ttttatcctt
    17221 tattttttta tctatcttca aataaaaact tgatatactt aaatagtccc cattttttag
    17281 gttttcatta cttactatta ctcctaactc atatccttga tataaattct tcgctttaca
    17341 ataattggtg aaatctatta attccatcat aaaatcacca ttactcatat tactcaaatc
    17401 acttgataaa actaaatatt cttcagactt tttggatata taaatttctt cactattcat
    17461 tttagaaatt ccttcgactg ttatatttat tctattttgc atcttagaac ttatctttcg
    17521 taattttttt attttttctc ttatcttttt ttcttcttct ttaagcattt tcacacaaag
    17581 ctgaggagtt ctattttgta tatactcttt tatttcctct aaactcattc ctatttctct
    17641 taatgattgt aatatatcca atgtatcata ttgataaact gagtaatatc tataattatt
    17701 tttatcttta tattcaggag aaaaaatccc tattttatca tagaaaatta atgtctgttt
    17761 agatatacca cacaatttag caaattcacc tgttgtaaaa tactgttttt ccaagggtta
    17821 tgcactcctt ttatttttat tatatatata gttactatat actttagtat ttactaaatc
    17881 aagtagttat tattttagaa aatcaatatg tttttgattt taaagattaa atgtcagtta
    17941 aatattttat ccatcccagt aataaaaaat atacaccctc tgaactttta gatattatat
    18001 ttatgcttgt tagagcggaa attgtaaatt gtatctacct cttactattt tatttatgat
    18061 atattacttt ttaatctaaa aagagatatc tatttttatt ctagttatct ctttttagat
    18121 tcctttaagc atatgaatat tccaatttaa cacctattga agtgctattt tcttatatca
    18181 caatttataa ttgatatatc ttcaatataa taatctaatg tatttttgat tttaaagctt
    18241 ttcagtaata ttattgataa attagaatta attatctagc tttaaaaggt caaaaatttc
    18301 agttgccgtc tgtggttttc caaataaata tccttgtatg taatctggct tcatttgttt
    18361 aactaaatca tactcttctt gtgtttctac accttctaaa catactttta ttcctacatc
    18421 atgacaaatt gcaacaataa aatgtataaa tgtagcatca aacttactct ttaatatatc
    18481 tttaacaaat actctatcaa ttttcactac atctattgga gaaaatttta aaacttctaa
    18541 agacgaatat ccagtaccaa aatcatccat agcaatataa attcccaaat cttgtaaagc
    18601 cttaaactta aactgtaaaa tactcatatt ttgtactgta tgactttctg tcaattctaa
    18661 aacaagattt tgataaggaa acttttcatc atctatgatt tttactatat cttcaataaa
    18721 tgtatcttct aatatttgaa cagctgaaac gtttacactc actttaaaaa aaggataata
    18781 atcaattaat cccttaaaag tacgtaacgc cattctaagc acccatgctc ctacagtatt
    18841 tatcatatca tgttcttcta atattggaat aaattctaag ggagaaattg ctttatattt
    18901 atcattagtc catcttaaaa ggacttctac acctattatt ttatgacttt gtgtatctac
    18961 ttgtggctgg aatcgaagac taaatcccct aaaatcatca ttaattgatg cttttaaatc
    19021 ccgcatcatt tctaatgaat acattttatt ttttaatatt tcttgtgaga aaaaaacaag
    19081 cctgttttta ccatgctctt ttgcatattg taaagagtaa ctagcatatt tataaagttc
    19141 ttttactgta tctccgtgct ttggatatat tacacaacca gcagaaattg ttatatttaa
    19201 tccatatttt ctccatagtt gaaggtgtat aatcatattc tgtatttgat tgtataaagt
    19261 tagaatttct ttttcttcaa cattatcaac aataattccc atttcatctc catcaagttt
    19321 ataaagttca gcattatctg gtaatataga ttgtatagac tgtgctagtg tttttattat
    19381 attatctcca aaatctctat catacatctc attaacattt ttaaaatcat caatatttaa
    19441 taatactatg cataattttt caatcatagg atttgaaatt ttatcttcaa atacactcat
    19501 aaattcatga tagttcaata actgtgttaa tgaatcaatc ttattttgtt tacctaattg
    19561 tgtcattatt cctgcaaaaa tacttggttt tccaaattca tcacgcatta gttgccctct
    19621 acatcttaac catatatatt ctccactgcg atttttagcc ctaaattcca ctgtatgata
    19681 gtccatctgg tttttaccta tttcagtatt tgacttataa aaacgtttcc agtcttctgg
    19741 atgtacaatt ttttcccagt aaataagagg attttcaaca atttctcctg gtaagtcaaa
    19801 taattcaact tgtgatggtg aatatcgaaa tactcctgtt ttcatattac aaatatatat
    19861 atattcatct gtacctctaa tcaaagcatc ataaagcaat gtagaattat aatcaaaatt
    19921 gtctattgag acattaaaaa ctcctttttc ttcttcagta tttgatattt tatgaaattc
    19981 attaggctta atttgtttat ttaagcttgt tgagtttgta atatctattg attggtgcat
    20041 atgaacaact gtgccatctt gccatgtaat taaactatca taattttcaa aaacacaatt
    20101 tagcttatta catttttcat accattttat taatactcct tttttatcat tctttaacaa
    20161 ttctacaact ttacaaaaac tacaagtaga atttttttca ggatataata cttgccaaca
    20221 aacttttcct tctggatcta aaatgttata ttcttctttc atttttttat tcataaaaac
    20281 aatttcatta gtatgaatat tcgtaacata tatgttaatc tgtagttgat ttaatataac
    20341 ttcaaaatta tgtttgttca tatacttatc tcctccatag ttattgctct ttataacaac
    20401 attgttaaaa ttattctttc cagaagattt acttataatt tatatatata cacaatacct
    20461 taatttaatt agaaaattta ttacttcata ttttaaatta taattgatt
//
LOCUS       DAMJVM010000063        19880 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.066, whole genome shotgun sequence.
ACCESSION   DAMJVM010000063 DAMJVM010000000
VERSION     DAMJVM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19880
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.066"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            321..779
                     /locus_tag="PC523_002417"
     CDS             321..779
                     /locus_tag="PC523_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2475931.1"
                     /translation="MEFINFVSDKVIELKMDCKTKENFFKEIHNKVFDLGFVKEEFGE
                     KILERENVFPTGLNLGDYGVAIPHTDAEYIKEQFIAVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            832..1107
                     /locus_tag="PC523_002418"
     CDS             832..1107
                     /locus_tag="PC523_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2475932.1"
                     /translation="MKKILVACGAGIATSTVVCDKVEKLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            1166..2440
                     /locus_tag="PC523_002419"
     CDS             1166..2440
                     /locus_tag="PC523_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2475933.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKLVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLLLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            2571..3623
                     /locus_tag="PC523_002420"
     CDS             2571..3623
                     /locus_tag="PC523_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2475934.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYANIKATNVLPIGDMDFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDVNPVEK
                     IKELTDGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            3623..4654
                     /locus_tag="PC523_002421"
     CDS             3623..4654
                     /locus_tag="PC523_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDF2475935.1"
                     /translation="MKAVVKTKPGYDNVEVLDVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCQTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMASGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            4745..5557
                     /locus_tag="PC523_002422"
     CDS             4745..5557
                     /locus_tag="PC523_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDF2475936.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVMVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            5560..6480
                     /locus_tag="PC523_002423"
     CDS             5560..6480
                     /locus_tag="PC523_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDF2475937.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGVNVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            6655..7101
                     /gene="rpiB"
                     /locus_tag="PC523_002424"
     CDS             6655..7101
                     /gene="rpiB"
                     /locus_tag="PC523_002424"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDF2475938.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEIEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            7131..7796
                     /locus_tag="PC523_002425"
     CDS             7131..7796
                     /locus_tag="PC523_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF2475939.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVRADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYIGLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIKELKE
                     SIK"
     gene            7906..9408
                     /locus_tag="PC523_002426"
     CDS             7906..9408
                     /locus_tag="PC523_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDF2475940.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVRKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSAVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGSIMITASHLPYYYNGLKFFTESGGLEKADIKEMLDIADKNDSVDYEKANKKGKV
                     ISKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRYKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAREEKEVRIGINKEDFRPYAEDILKDLDSHVRSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLRGL"
     gene            complement(9720..11312)
                     /locus_tag="PC523_002427"
     CDS             complement(9720..11312)
                     /locus_tag="PC523_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2475941.1"
                     /translation="MVTKLKNLIINDSKAKFILLLLLFLLISILFEAVYDLNNNFFYN
                     INYYYKEFTIPYFYFFGTPLHLSKVELEFVCIGVILSSFLLIVLCFILYKKNKQTKVK
                     FLENVISLFSKVKIELTLILILILLILRFHAFTSNKMFYNTNLIIYNFIIISIVYIIY
                     ENIKYNNYNLKDMSITYVFFSNLLSVYNRKTINKKLAIVFLISIVMQAIIQNMVLIFF
                     MYIYYAEAIAIVILFTLVNTVFHLYIYNFLSAKFNYINNISKNVKNIENGDLKYKLEV
                     IGNDEISDLSKSINNITDGLENAVDSSIKNERMKTELITNVSHDLKTPLTSILSYVDM
                     LKNNDFDKETVNDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQ
                     SIVEFEDKIQNSNLDFIIETPEHAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYID
                     MFIKEENIILTFKNISNDKLNLKPEELVERFRRGDVSRKTDGSGLGLSIAQNIIELEN
                     ADMEIIIDADLFKVMLTFRRVN"
     gene            complement(11281..11997)
                     /locus_tag="PC523_002428"
     CDS             complement(11281..11997)
                     /locus_tag="PC523_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2475942.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLKPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKVIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSREIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            complement(12540..13340)
                     /locus_tag="PC523_002429"
     CDS             complement(12540..13340)
                     /locus_tag="PC523_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2475943.1"
                     /translation="MTFKNKKFTISKYRILSIILALCLILTAFSSYMNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTDIQK
                     SEYRNREFLASLNSLGVRRENIILLPETNTNGNTDFNYMEKIAINFEENFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEDREGIGYKSDHKSFDKLLRNKDVPAILHTPNL"
     gene            complement(13652..14383)
                     /locus_tag="PC523_002430"
     CDS             complement(13652..14383)
                     /locus_tag="PC523_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475944.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIIGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLYDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEICAKNFPKFIKREFDNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTVSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            complement(14370..15065)
                     /locus_tag="PC523_002431"
     CDS             complement(14370..15065)
                     /locus_tag="PC523_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2475945.1"
                     /translation="MLKIRNLSLDLPNFKLQENSLNINNHEYFVLLGSSGSGKTLFLE
                     TLAGRYPSAKGSVSFKKNLISSLPPENRNIGFVYQNFELFPNMNVEDNISFPLKLRKI
                     PIDEQILKVNHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKHKGESFFYEYI"
     gene            complement(15067..15858)
                     /locus_tag="PC523_002432"
     CDS             complement(15067..15858)
                     /locus_tag="PC523_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2475946.1"
                     /translation="MKNKFFTILLTGFFAVVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKILKLDFVFTKQGIILAQVFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFKFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            complement(15868..16680)
                     /gene="modA"
                     /locus_tag="PC523_002433"
     CDS             complement(15868..16680)
                     /gene="modA"
                     /locus_tag="PC523_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2475947.1"
                     /translation="MFKKFSVLILSMIISLSITACNSKENKANNESNAKVTTANDSKE
                     KETLFVYSGAGLKKPMDEIAQKFEKENNIKIEYSYAGSAQLIAQIETSHKGDAFIVGS
                     EPIYSKAFEKKLVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNRDGVKLIFGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIASDEAKQIFKKYGFEPVK"
     gene            complement(17291..17491)
                     /locus_tag="PC523_002434"
     CDS             complement(17291..17491)
                     /locus_tag="PC523_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2475948.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(17716..17892)
                     /locus_tag="PC523_002435"
     CDS             complement(17716..17892)
                     /locus_tag="PC523_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475949.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFIIEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(17946..18035)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(18318..18518)
                     /locus_tag="PC523_002436"
     CDS             complement(18318..18518)
                     /locus_tag="PC523_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2475950.1"
                     /translation="MPKFICNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(18520..18870)
                     /locus_tag="PC523_002437"
     CDS             complement(18520..18870)
                     /locus_tag="PC523_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HDF2475951.1"
                     /translation="MIQKKFFMGFCGFIGFLSLRYFSSGNIADLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKSAMAFTGQFAIIELFILWCITIVSRNVELMCVLLSIAYAIT
                     LNVYAIKLYILEEK"
     gene            19232..19453
                     /locus_tag="PC523_002438"
     CDS             19232..19453
                     /locus_tag="PC523_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475952.1"
                     /translation="MNKYNKTLKKNKIFQGISRKATFFDNSPMKNSFGVMKRECIIKK
                     YKQLFESFFVIIKRIMRGLRNLDMKVLLS"
     gene            19384..19761
                     /locus_tag="PC523_002439"
                     /pseudo
     CDS             19384..19761
                     /locus_tag="PC523_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427607.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
BASE COUNT         7605 a         2433 c         3043 g         6799 t
ORIGIN      
        1 tcataataaa taattttata aaatagttgt taaacataaa aaaccattta taaaaaacaa
       61 aaaaatcatt tctctaacaa aaaaaaccac aaaaaatata aaaaagtatg gctagttaag
      121 tgattaacaa gtaaaaatta aaataaaaat caaaaaaaat ttatcaatga aagtcagcta
      181 aaatcctaga agagagcatt attttaagga aaacattttc ttttgtttga caaaaaaagc
      241 cactttttgg cacacaacat gctttatata aagtcaagaa aaactaaggt acaagacaaa
      301 ggcaattagg aggtgtaaat atggagttta taaattttgt aagtgataaa gttatagaac
      361 ttaaaatgga ttgtaaaacg aaagaaaatt tctttaaaga aatacataat aaagtttttg
      421 atttaggctt tgtaaaagaa gaatttggag aaaaaatatt agaaagagaa aatgtatttc
      481 caacaggatt aaatttaggt gattatggag tagccatacc acatacagat gcagaatata
      541 ttaaagagca atttatagca gtctgtactt ttaatgaacc agtagtattt agttctatgg
      601 aagaccaaga tgaaaaagta cctgtaaatt tggctttcgt attaggactt aatcaaccac
      661 acagtcaatt atctgtatta acagaattaa tgggaattat gcaaaataaa gagttagttg
      721 ataagttgat gagttctact gataaagaag aagttttaaa gacactaaag tctttataaa
      781 aataaaaaag tactaaaaat aaaaaataaa aattattagg aggaaataaa aatgaaaaaa
      841 atattagtag catgtggagc aggaatagca acatcaacag tagtttgtga caaggtagag
      901 aaattagtta aggaaaataa tatacaagca gaagttatac aatgcaaaat agctgaatgt
      961 gcaacaaagc aagaaggagc agatttaata gtatcaacaa ctatattacc aacaacttat
     1021 aatataccag caataaaagc aacagcatat ataactggag taaatacagc agcattagat
     1081 aaaaaaatat tagatgcttt aaaataatcc tatcatagat acattgaaat aatattttat
     1141 aaaacagaaa aaggaggatt tataaatgga taaattatta gcagtagtac aatacatttt
     1201 aaatatggga ccgacagtaa tactaccaat aacaattgta gtactaggaa tgatatttag
     1261 aattaagttt acaaaagctt taaagtcagg tcttacaatc ggtataggat ttgtgggtat
     1321 aaacttagtt gtaaacctat taacatcaac attagggcca gcggctcaag gaatggttga
     1381 aagattcgga cttaatttaa cagtaatcga tgttggttgg ccaacaacag catcagcaac
     1441 atgggcttca ccagttgcac caatattaat accagtttgt ttattagtaa acttactatt
     1501 attatacttc aataaaacaa agatattaaa cgttgatata tggaattatt ggcactttat
     1561 agtagcaggt gcggttggat atatagtaac aggaagctat gtttgggcta tattctgtgc
     1621 aatattaatg gaattattag tactatttat tgcagaaaag acagcaaaaa atgttcagga
     1681 gttttatgat ttagaaggta tagcattacc aactggttca acagcttcat ttgctccact
     1741 tggatattta gtaggtaaac ttgttgaaaa aataccagga ataaataaaa tacaagcaga
     1801 cccagactct atacaaaaaa gatttggtat atttggagaa ccaatgatga tggggattat
     1861 actaggtata atacttggag cacttgctgg atatgatgta gcaggaatat ttaatgtagg
     1921 tatatcaatg ggtgcagtaa tgttacttat gcctagaatg gttagaatat taatggaagg
     1981 tttaattcca atttcagaat caataagaga tttcttacaa gcaaaatatg gagacagaga
     2041 actttatata ggactagatg cagcagtagc aactggacat ccagcagtta tatcaacagc
     2101 tcttatacta gttcctataa ctttactttt agcagttata ctaccaggaa acaaagtctt
     2161 accatttgga gacttagcaa caataccatt ttatatggca tttatagtgt gttttagaaa
     2221 aggtaatata gttcaatcag ttataacagg aacaatagta atagctatat cactttatat
     2281 ggctacaaac tttgcagatg ttcatacaca attattacaa caagcaaata tgatgccaga
     2341 aggaacaaca atggttacaa gtatagatac aggtggaaac ttgctaaaat ggggaatatt
     2401 gaaattatct caattagttt cttcatttgg aatattctaa tatgtagtta atatagatta
     2461 ctctttttta gtaaagtatg cgaaaattta atatttagca actactaaaa aagagtaata
     2521 ttttttataa atttttaaag aatacaaaat acaagaggag gagatttatc atgaaagcag
     2581 cagtattaca tggcacaaat gatatgagat ttgaagatat agaaataaaa ccatgtgaaa
     2641 gtgatgaagt aaaaataaaa gttatggcag caggaatatg tgggtcagat cctccaagag
     2701 tattaaaaca ttggaaatat ccagttccag ctataccagg acatgagttt tctggagtaa
     2761 ttgctgaagt tggtaaggat gtaaaaaatg taaaggttgg agatagagta gttgcaattc
     2821 cttttatacc ttgcaatgaa tgtgaatatt gtaaaagagg attattttca ttatgtgatg
     2881 accatggaat gttaggagct aaaagttttg gagcttttgc agagtatgca aatataaagg
     2941 ctacaaatgt acttccaata ggagacatgg actttgaaga tgctgccatg atagagccac
     3001 tagcagttgc tatgcatgga gtacttaata taggtgttca agttggagac actgtagcag
     3061 taatgggtag tggaacaatg ggacagttag taatacaagg acttaaaata gcaggagcag
     3121 gaacaattat agcagtggat atatctgata ataaattaag agaatcaaag gaattaggtg
     3181 ctgatataat aattaatgct aaagatgtta acccagtaga aaaaataaaa gaacttactg
     3241 atggaaaagg tgttgatata gctcttgaat gtgcaggttc aaaaataact caagaacaat
     3301 gtttacttat aactaaaaag aaaagtaaga taggattttt aggaatagca tattcagata
     3361 taactttatc agaagaagca tttgaaaata tatttagaaa agaactagag ttaaaaggtt
     3421 tttggaactc ttattctgca ccattcccag gacaagaatg gactaaggga atcaatctag
     3481 ttaatgaagg aaagataaaa ttaaaagaaa tggtatctca tagattctca cttgaagata
     3541 cttacaaagc atttgaaatg ataagagata gaaaagaaga atttaataaa atattaattt
     3601 taccacaagg ggttgaaaaa taatgaaggc agtagttaaa actaaaccag gttatgataa
     3661 tgtagaagtt ttagacgtag aagaaccaaa agctacagga gacaaggtta aaataaaagt
     3721 agaatatagt ggtatatgtg gttcagatat acattcattt aaaggtgaat atgcaaatat
     3781 aaaagcccca gtaacattag gccatgaatt ttctggaatt gttgtagaag taggagaaga
     3841 tgttaaaaat ataaaagtag gagatagagt tacatcagaa actacttttg cgacttgtga
     3901 aaaatgtata tactgtgaaa ctaaagatta taacttatgc caaactagaa aaggtattgg
     3961 tactcaagta aatggaagtt ttgctgagta tgtactatca agagaagaaa gtatacatgt
     4021 acttcctgaa aatgtatctt tattatcagc agcccttaca gagccactag cttgttgtgt
     4081 tcatgcagct cttgaaaaga ctactataga aagctctgat acagttttaa taataggacc
     4141 aggaccaatt ggtttattat tagctcaagt tgttaaatct caaggagcta cagttataat
     4201 gtctggtgtt acaaaagaca aggaaagatt agaaatagct aaaaatcttg gagtagatag
     4261 aactgtaaat gtaatggaag aaagtttggc agatgtagtg aatgaaatga ctgatggaaa
     4321 tggagtaaac aaaggatttg attgttctgg atttatgcca gcagttaacg aagctttaag
     4381 attgcttaga aaaaaaggta ctttcgtaca agttggaatc tttgctaaaa aattaaatga
     4441 aatggaccaa gaagcaataa tccaaagaga gttacaatat ataggaagta gatctcaaaa
     4501 gccaagttct tggattacag cattagattt aatggcaagt ggaaaagtaa atacagaagc
     4561 tttagttact agaactgttg gtttagacga atttaaagaa ggtattacag ctttaatgaa
     4621 cggggaagaa ataaaggtag cagtaaaatc ttaaaaaaac atttgtatta ttttaaaaaa
     4681 aattgtaaaa tctactgtag gataaaaaat gtaagatctt aaaaaataac aggaggaaga
     4741 aagtatgagc actcttagag atcattctaa taatattaga aaaaacataa taaaaatggt
     4801 tgcagaagct aaaagcggac atccaggtgg ttcattatca atagctgata tgttgacagt
     4861 attatatttt gataagatga acgtatgtgc agaaaatcca aaaatggctg atagagatag
     4921 atttgttcta tcaaaaggtc atgcagcacc agcactatat gctacactag cagaaaaagg
     4981 atttttccca gaagaagaat taataacact aagaaaattt ggttctaagt tacaaggtca
     5041 cccagacatg aaaaaagtag caggaatcga tatgtctact ggttctcttg gacaagggct
     5101 atctgcagca aatggtatgg cacttgcagg aaaattagac aacaaagact atacagtata
     5161 tactatatta ggtgatggag aaatccaaga aggtcaaata tgggaagctg caatgacagc
     5221 atcacattac aaattagata atttaattgc atttgttgac ttaaatggat tacaaataga
     5281 tggttctaat gaagaagtaa tgaatgtaaa tccagtagat gacaaattca aatcttttgg
     5341 atggaatgta atggtaataa atggacattc ttttgaagaa ataggaaatg cagttgatga
     5401 agctaaaaaa gttacaggaa aaccaactgt aataatagct aaaacagtaa aaggtaaagg
     5461 tgtttcattt atggaaaata atgctgcatg gcatggaaca gctccaaatg cagaacaagc
     5521 agagcaagca ttgaaagatt tagaaggagg tctataggaa tgggaatagc aactagagaa
     5581 gcttacggac aagtgttaaa agaacttgca gaaaataaag atatagtagt attagatgca
     5641 gatttaggaa aagccacaaa aagtatatca tttaaagaag ttgccccaga tagatttttt
     5701 gatatgggga tagcagaagg tgatatgatt ggtacagcag caggtcttgc tacttgtgga
     5761 aaaatacctt ttgcaagtac gtttgcaata tttgcagcag gaagaggcta tgagcaaata
     5821 agaaactctg tagcatatcc aaatttaaat gttaaaatag cagctactca tgcaggtgta
     5881 acagtaggtg aagatggtgg tagccatcaa gctatagaag atatatctct aatgagaggt
     5941 ataccaaata tggttgtatt aaatccagca gatgctttag aagcaagaca agctattctt
     6001 gcatctattg attataatgg acctgtttat ataagattag gtagagctgc aactcctgat
     6061 gtaaacagtg aaaattataa atttgaaata ggaaaaggaa ctgttttaag agaaggttct
     6121 gacataacag tgatagctac aggaataatg gtagcaaaag ctttagaagc agcagaagaa
     6181 cttgctaagg aaggtgtaaa tgtagaagtt gttaatatat ctacaataaa accattagat
     6241 gaagcattaa taaaagaaac tgctaagaaa actggaaaag tagttacagc agaagaacac
     6301 agtataatag gtggtttagg ttcagctgta tgtgaagctt tagcagaaac taaagatgta
     6361 gtagttagaa gaattggtgt taaagatgta tttggtcaat caggtacacc agctgattta
     6421 ttaaaacact atggcttaac tacagaagat atagtaaaaa atataaaaga attgttatag
     6481 atataaatat atctaaataa caaattgatt tgacataaat caattaaagc tgtcttaaaa
     6541 atagaaagaa atttctacaa tgggacagct ttatttttac aatactttat ttttatgata
     6601 attgtaaaat agtataaaaa taatcaaaaa aattatagaa agaggtgcta tttaatgaaa
     6661 atatctattg gatgtgacca tggtggttat gaattaaagg aatatataaa ggaaaactta
     6721 ataaataaag gtatagaaat tgaagatgtg ggtacaaatt caaaagaaag cgtagatttc
     6781 ccaatatatg caaaaaaggt aagtcaaaat gttcaaagta aaaaatctga tttaggtata
     6841 ctttgttgtg gaacaggaat aggtatgtct attgcagcaa ataagtttaa aggaatcaga
     6901 gcagctgttg tttctgactg tttttcagct aaggccacta gagaacacaa taatacaaat
     6961 gtactttgtt taggggaaag agttattgga aaaggattag caatgagaat tgttgaagaa
     7021 tggttaaatt caagttataa tggagaaaga catgcaagaa gattacaaat gattgatgat
     7081 atagaaaaag aaaatttata aatatataat ttataataag ggggaacgtt atgtctatta
     7141 tttgtgcttc aataatgtgt gcagaccaaa tgaagttaaa agaggaatta gaagctcttg
     7201 aagaagcagg agtaaaatta cttcattgtg atgttatgga tggaatattt gttaaaaact
     7261 tggctatggg gccagagtta ttaaaatcaa taagtgaaaa tacaacaata cctttagaca
     7321 tacacttagc cacagaaact ccagataagt atattgatat gatgtcttat ataaagccaa
     7381 aatatatttc ttttcatgta gaatcaagca caaatgtaag agctgatata caaaaattaa
     7441 gaaattatgg tatagggcca gtattagcaa taagccctca aacaagtgtg gataaaatag
     7501 aagaatatat aggtttagtt gagggtatct tagtaatgac agtaaatcca ggatttgcag
     7561 gtcaaaagtt taatctttct gtattggata agttggataa acttacagag atactaaaag
     7621 attatgataa tcctccattt atagaggttg atggaaatat aaataaggat actattaaac
     7681 ttatgaatgg taaaaaagta gatatatatg ttgttggtac ttcagcttta tttaatgaca
     7741 agcctccaat ttcatataaa gataaaataa aagaactaaa agaaagtata aagtaactat
     7801 gtaataaaat tataatatgt ttctatttaa tggtaaaatt ttaggtagaa acaagaaatt
     7861 ttgtaatatt taataaaaat tttggttgga taaggagagt tttttatggg agacctatat
     7921 aaactgcaaa atggtactga tattagagga atagcatatg aaaatgattc taaagaagtt
     7981 aacctaactg tagaagaagt aagaaaaata gctaaagcgt ttcatatttg gctgaaagaa
     8041 aagactaaga aagataaagt aactgttgct ataggaactg attctaggat aacaggaagc
     8101 caatttagaa gtgctgtcat tgaaacttta actaatgatg attgtaatgt aattgattgt
     8161 gagattgcaa caactccagc tatgtttatg acaactatta tggatggata taattctgat
     8221 gggtcgatta tgattacagc aagccactta ccttattact ataatggttt aaagtttttt
     8281 acagaaagtg gaggcttaga aaaagctgat ataaaagaaa tgttggatat agctgataaa
     8341 aatgatagtg tagactatga gaaagcaaat aaaaaaggta aagttatatc aaaaaactta
     8401 atagaagatt attctaattt attaatagat aaaattagaa aaggtgtaaa ttcaagtaaa
     8461 aattatgaaa agcctttttc tggacttaag atattggttg atgcaggaaa tggagctggt
     8521 ggattctttg ctgaaaaagt tttacacata ctgggagcag atacaacagg tagtcagttt
     8581 ttgaacccag atggtatgtt tccaaatcat atacctaatc ctgaaaataa agaagcaatg
     8641 gaaagcatat gtaaagctgt gttggacaat aagagcgatt taggaataat atttgataca
     8701 gacgtagaca gagctgctat tgtaggtaaa aatggaaaac caataaataa aaatgctctt
     8761 atagctgtta tatcatcaat agtattagaa gaacatccaa aaacagctat agtaacagat
     8821 tcgattactt ctgagggact tgctaagttt ataaatgaat tacaaggaag acatcataga
     8881 tataaaagag gatataaaaa tgtaataaat gaagctataa gattaaatag tgaaggtgaa
     8941 gagtgccatt tagctataga aacatctgga catgctgctt taaaagaaaa ttatttctta
     9001 gatgatgggg catatttaat agctaaaatt ttaataaaag tagcaaaact atccttagaa
     9061 ggaaagacta tagaagaatt aatagaaaac cttgaagaag ctcgagaaga aaaagaagtt
     9121 agaataggca ttaataaaga agattttaga ccatatgcag aagatatatt aaaagattta
     9181 gattctcatg ttaggagtat tgatggatgg tctgtagcac caaataattt tgaaggtata
     9241 agagttaatt gtgatgaatc aaatggagat ggatggtttt tacttagaat atcactacat
     9301 gaaccattat tagctttaaa tattgaatca aataaaattg gtggagcaga acaaatttat
     9361 tctaagttaa aacttttctt agaaaaatat gatttaagag gtctttaggg taaatagaaa
     9421 gaatatattt atatcgaaga atatatgtaa atttgtacct aagattacaa atattacaaa
     9481 attgtaatct taggtaacag agatgtaact gattatggaa acactatgtg atattataaa
     9541 tacagagaga tgaaatacat gctgaattat agagaaatat aatttaaaat gtattaatat
     9601 cacaccaaaa aggtataatc aaaaaatatg taaatataaa ccccaacatt tagaaaaaga
     9661 cctcgaagga ggtttttttc ttgtttaaat aacttaaaat atatttttat attaatgtat
     9721 caattaactc ttctaaaagt aagcattacc ttaaataaat cagcatctat aattatttcc
     9781 atgtctgcat tttctaattc aattatattt tgagcaatgg ataaaccaag accagagcca
     9841 tctgtttttc tagatacatc accccttcta aatctttcta ctaactcttc tggctttagg
     9901 tttagtttat catttgaaat atttttaaat gttaatatta tattttcttc ctttataaac
     9961 atatctatat agactctagt attaggcata gagtatttta gtatattgca aattagattt
    10021 tcaaaaactc tccaagtttt ttttccatct atcatggtaa aaacagcatg ctcaggtgtt
    10081 tctattataa aatctaaatt gctattttga attttgtctt caaactctac aatactttga
    10141 attaaaagtt ctcttatatc tgtttttgtt atattaaatt ctacatctcc actacttagc
    10201 ttagatgctt caaatatgtc atctattaat aattttaatc tttgtgattt tttatctaaa
    10261 atatttatat agtcatttac agtctcttta tcaaagtcat tattttttaa catatcaaca
    10321 tagcttaata tagatgtaag tggagttttt aagtcatgag atacgtttgt gataagttct
    10381 gtcttcatcc tttcattttt tatactagaa tctacagcat tttcaagacc atctgttata
    10441 ttatttatac ttttagataa gtcagatatt tcatcatttc ctattacttc tagcttatac
    10501 tttaaatctc cattctcaat attttttaca ttctttgata tattatttat gtaattaaac
    10561 ttagcagata aaaagttgta tatatataaa tgaaaaactg tgtttacaag tgtaaataaa
    10621 attactatag ctattgcctc agcgtaatat atatacataa agaatatcaa aaccatattt
    10681 tggataatag cttgcataac tatagatatt aaaaatacta tagctaattt tttatttata
    10741 gtttttctat tatacacact aagtaagttt gaaaagaata catatgttat agacatatct
    10801 tttagattat agttattata tttaatattt tcatatatta tgtagactat agatataatt
    10861 ataaaattgt agattattag attagtgttg taaaacattt tattacttgt aaaggcatga
    10921 aaccttaaaa ttaatagtat caaaattaat ataagtgtaa gttctatttt taccttagaa
    10981 aaaaggctta tgacattctc taagaatttg actttagtct gtttattttt tttatacaat
    11041 ataaaacata gtacaatcaa taaaaaactt gataatataa cacctataca gacaaactct
    11101 aattctactt ttgacaaatg taaaggagtg ccaaaaaaat aaaaataagg tatagtaaac
    11161 tctttataat aataattaat attataaaaa aagttattat tcaaatcata aactgcttca
    11221 aataatatag aaattaatag aaacagtagt aataataaaa taaattttgc tttagaatca
    11281 ttaataatta agtttttcaa ttttgtaacc aatgccccac accaccttta aatattttgg
    11341 ttcttttgga ttaatttcaa ttttttctct aattcttcta atatgaacag caatagttct
    11401 ctcacaacta taagcatcct cattccagac attttcataa atctcacgag aagagaaaat
    11461 tttacctttt cgtgacataa gaagagtaag tattttatat tcaagtggtg tcattttaat
    11521 ttcttcccca tctaaagaaa ctatttttgt atctgtattt agctctagtc caccactttt
    11581 tagaatataa ttagtatcat ttgagttatt tgtattacta aaatcaccta aatttatgta
    11641 tcttctaagt tgagatttta ctctagcagt aagttctaat ggattaaatg gtttagttat
    11701 atagtcatca gcacccatat taagtcctat aactttgtcc atatcttctg attttgcaga
    11761 taagagaatg attggtatat ttcttttttc tcttatttta agtgtagttt taagcccatc
    11821 taaattgggc atcattacat ccattattat taatttaata tcattttcga ttagtatatc
    11881 caatgcttca agtccatcat atgcctttaa aacattaaag ttatcaggtt ttaaatagaa
    11941 ttctatagca tcaactatct ctctatcatc atctacaacc aatatatttt gattcaattt
    12001 attaacctcc ttgaaatttt atttttaacc aatctttatt ttataccaaa aagaagtatt
    12061 agaaaacagt aagcatattt gttatgaaat aaaaactaat acttttaata tattgtcaat
    12121 gcatatagta atttttaaag catagatgta tatattaata tgaagttaaa agtttatgaa
    12181 aatatctagg ataatttatc acactcctta tgtcatttta tgtttattaa ttttttaggt
    12241 ctaaacatat actaacaaat ataaataaca aatgagtttc ataattatta agaatttata
    12301 acaaatattt attttgatgt attttaatat aaataggtag tttaatgtag atatgcataa
    12361 aacctttaag aagaaaactt ataggtttca ataataatat ttttatatat tttaagacat
    12421 gatgaatcta gatatgggga atagttggat gagaaaaagt agtatagcac aaaaaagtgc
    12481 tatatcacaa tctcaaataa tttacttttt ataatttaag ctaaaagaat aaatatttct
    12541 tataaattag gtgtatgaag tatggcagga acatctttat tccttaataa tttatcaaaa
    12601 gatttatgat cagatttata accaatacct tctctatctt ctaactcatc aacaattttg
    12661 tattgttgac aggcattttt taccttttca tagtcttcag tagaatgagc tttataaact
    12721 attttattat taaaagttat tttatcagca aattttggtt ttacaaaata tttggcatct
    12781 tttattttac cagcattata taaattttgt ataacagaac cattttttaa atgttgcaaa
    12841 tggtcgtcta acttataagt atgtgctata tgagttacat ttttaaaatt ttcttcaaag
    12901 ttaatggcaa ttttttccat gtaattgaag tcagtattac catttgtatt tgtttctggt
    12961 aataatatta tattctccct ccttacacca aggctattaa gagaagctaa aaattctctg
    13021 tttctatatt ctgatttttg tatatctgtt agattttcat actttttata tttatcaaat
    13081 acagatatgc cacatccagt agataccaaa accacaaata catgattttt tcctctttct
    13141 tttatagcat taacaatagc actcccagcc cacaacactt catcatcttg atgttgaaca
    13201 tagaagacaa cataatcttt aaatttttta tgactataat tcatatagct gctaaaagca
    13261 gttaaaatca agcataaagc tagaatgata gataaaattc tatatttact tatagtaaat
    13321 tttttattct taaatgtcat ttaatcatct cctgaaaagt tgtcttagtt atattatcaa
    13381 acaaagtgct aaaaattatt tcttaaatac tgaaaaatat agagtagatt aatggaataa
    13441 aattttttta ttaatttaaa attaagaaaa tatttatgta taaaaccaat caaaatatta
    13501 attagtgata ggaattgaga tttaaaggga ttgtacattt atttgagatt catttatata
    13561 gcattacttt agtattgaga ataaaagtaa taactattta aatttaaaga gtacacattg
    13621 atatttgtgt actctttaat gatgtgattg cttatttact acaaaaacat agtcttttta
    13681 aacctaaaat atttgatgca ccagctatcg tagtgccata atagtaaact ggtttattaa
    13741 gtgatagaaa atttataatt gagttattac ataatgtact tactgtgcaa atcactaaat
    13801 cagcccaaga tattacatct tcataatctc gtataccatc ttcaatctta acattatact
    13861 gaatagtatc tacaaattca ggatttaaat ctaatactct tgtttcgaaa aagtccttaa
    13921 tattatcaat tatggctggt tgatatccta taattgctac ttttgggtta tcaaattccc
    13981 tttttataaa cttagggaag ttttttgcac atatctctgg ttcattattt ttacaatgaa
    14041 tcgttctatc cgtcttacct aagtatttca taacagcatt taatgatgct ataaaaagac
    14101 ttctgtcatg aggattatca tataaatcta atgataatat atcttttaaa gaaccttcaa
    14161 acgtagatgg agtagaagta aatgcttgtc ctttagctcc tttaaagtca gcttcaagca
    14221 ttatttcttt accaattata attggaaaat ctttacgctt agtatttcca attgcttctt
    14281 ttgaagatag cgatttggat acaatactta tcatatccat atttaaatta tatttatcta
    14341 cttgtactaa tagataatct ctcaaaatat caaatatatt catagaaaaa agattctcct
    14401 ttatgtttta tttcttcatt taaatcaaaa atatgattta tttttccatt gttcattatt
    14461 cctatcttat gagcaaaaaa cattgcttca gaaatatcat gagtaacatg aatcaccgtt
    14521 ggtttgtatt ttatgtagac atccttcaaa attttgctta tatgtttttt tgtaatataa
    14581 tctaatgaac tcataggttc atctaaaagt aatatatcag gttcagctat taaagcacgt
    14641 gcaaaagcaa ctcgttgctt ttcccctcca cttaaatttt taggatatct attttttata
    14701 tcctctatct gcattaattt agacaagtgg ttaactttaa gaatctgttc atctataggt
    14761 atttttctta atttaagtgg aaagcttata ttatcttcaa cattcatatt tggaaataat
    14821 tcaaaatttt gatacacaaa tcctatattt ctattttctg gaggtaagct tgatattaaa
    14881 ttttttttaa atgatacact tcctttagca gatggatatc ttcctgctaa tgtttctaga
    14941 aatagagttt tacctgagcc actagatccc aataaaacaa agtattcatg gttattaata
    15001 tttaaactat tttcttgtaa tttaaaatta ggtaaatcaa gagataaatt tcttattttt
    15061 aacatattaa tttttatcaa cctctctatt aaaaaatatc tcaaaaagca tcaagcatgt
    15121 aagtgagata aatatcaaaa ttacagctat tccaattgca atatctatat caccagtaga
    15181 catatttaaa tatatagatg taggaataat ttcagttttc atacgtgtag aacctgctat
    15241 catagctact gcaccaaatt caccaagtgc tcttgaccaa gtcattacag ttgcagatat
    15301 gattccagtt tttagatttg gaagaattat aaatttaaat gcttcccaac tattacatcc
    15361 aagagtacgt gcaacaaata tcatcttttc gttactttca tttagtgaag tgtgaagtat
    15421 tttaatagta agtggaagat ttacaaatac ttgtgccaaa attatacctt gctttgtaaa
    15481 tacaaaatca agcttaagaa tcttgtaaat ggaatctcca atccccattc ttccaaacaa
    15541 aagcaagaga gcaattcctg atactaggtg gggaagagac atagggagat ttattatctg
    15601 tataatcagt tttttaaatg gtaatttagt aatatgtaaa aagtatgcta ctggaatagc
    15661 taacaaaagg catactatag tagatattaa agatgtttta atactaagtg ttattgcata
    15721 ctgtacctcc actgatttta agcatatagg aacataactt atccctttaa tgattaacat
    15781 aagaagtggt gatacaataa ataaaaagac tacagcaaaa aatccagtaa gtagtatagt
    15841 aaaaaattta tttttcaagt ttaatcacta cttaacaggt tcaaaaccat attttttaaa
    15901 tatctgttta gcttcatctg atgctatgta gtttataaat ttattagcta aatctttttt
    15961 atcactatag tttacgattc ctgctgatat gagttggtct atgtttttat cttgagcaat
    16021 ttctataact tctacatcct cattgccaaa gactgaatct tttgtggcta ttgtagcatc
    16081 tgctttactt gatgttaact gtacaatcat ttcatttaca gttgcagctg tagagacaac
    16141 attatcattt atagatttta aattattttt ttcaagtatt ttttgcgtag tcttacctat
    16201 ggcattagac tctttgtctc caaaaataag tttaactcca tctctattta agtcttccag
    16261 tttttgaata ttagctggat tccctttagg tacaactatt gcaggagtat gatgtgcaac
    16321 tgttttatgt tcacctacaa gttttttttc aaaggccttt gagtaaattg gttcagaacc
    16381 aacaataaat gcatctccct tgtgacttgt ttcaatttga gctattagct gtgcagagcc
    16441 tgcatagcta tattcaattt tgatattatt ttctttctca aatttttgag ctatttcatc
    16501 cattggtttc tttaaacctg cacctgaata tacaaaaaga gtttcttttt cttttgagtc
    16561 attagctgtt gtcactttag cattagattc attattcgct ttgttttctt tagaattaca
    16621 tgcagtaata cttaatgaaa taatcataga taaaattagt actgaaaatt ttttaaacat
    16681 aatatattcc cccttggtaa attagtgtaa attctttttt tagtatatca tactaagaaa
    16741 acgatgtcta agcttttgtt atgttttata atgttttgtt atgttttgtc atgtgtggtt
    16801 aagagaaaat ctatctataa tgtcattttc atagagagtt atttcatttt taacttttat
    16861 taatacttta aagtatttta aatttaaaaa ctatttttaa attaatcgat tgtagtcttt
    16921 ttatttttta aatgaattta ttttatattt atacatattt cattaacaaa tatagctctg
    16981 aaatatataa atatgtttta attacttttt attgataatt tattagaatt gaatagaaga
    17041 gaaagtatat gtgtttagga gttagttgaa agttttggta tgttacaatt aaattattct
    17101 attatatagt ttaactaaaa aaatatttgt aagtttaagt atatacttta taaatatttt
    17161 tttgtgagac ttattttata aaaagtgttt agactaagta ttctcttatt aagggatttt
    17221 tttacttatt tacagaatat tatcaataaa aaaaagaccc tcaaaagagt ctttccgaaa
    17281 aatctatata ttataatata gttatatttt ctgcttgagg acctctagcg cctttaacta
    17341 tatcaaagct tactttttga ccttcttcta atgatttaaa tcctgaagtt tgtatagctg
    17401 agaaatgagc aaatacatca tctccacttt ccattgatat aaatccaaat cctttttcat
    17461 tattaaacca ttttactatt ccattattca ttaaaaatac tcctattaac ctatatcaag
    17521 atatacttga tttacttgtg tattaccaca aatactattg tatcatagat taatagaaat
    17581 gtctatttaa ttataatttt tagttaacat ttttatttat tcttaatatg aaaataaatt
    17641 taaacatttt atattaccaa tatattaatg taaatgtaga taaaactaaa aagggttttc
    17701 tagaaaaaaa tctgtctaat tatacttact taccaatacc atcacttgat gcaagcaaga
    17761 ttctacaact ttttctccat actctattat aaaatcatct aactgtgtat ctataaaatc
    17821 acaataatca tatgaactac caatataagc agttaacata tgtgtacatt tatccatttt
    17881 atctctaaac ataatgtatt tatctcccta aaaaactttc atgaaaaaca aaaagaaaat
    17941 atctttttgg caactggctg acataactta ttttataagc gttagtccct atagctttgc
    18001 gtcactaaat ttctctagtt ttgccgattt agttttattc tacaaactaa taatacaata
    18061 gaattagtta ttattcaaca tatctagctg aaaaattaat aaatttatat attaatgtta
    18121 aattattttt tcaatattaa aaagagaggt gaacacacat tgataatttt ttacaaggta
    18181 tactagtaaa ttcagttgcc agtttaatat tttacttaac taataaatta ttaaaaaaat
    18241 aaaaagcccc tctgggagga gtgactaaaa attgaattta agtttgaatt taaaacgtgt
    18301 ttttaataat gcaaaaacta atcaaaaata aacaattcat caataggagt atctactatt
    18361 tttgatatat ctatcgctaa ttttaaagaa ggattatact tacctgcttc caaacgcata
    18421 atggtttctc ttctaactcc tactttttct gctaattgtt cttgagttaa ttcttttaat
    18481 tgtctgtatt tttttagatt acaaatgaat ttgggcatgt tatttttcct ctaaaatata
    18541 aagttttata gcataaacat ttaaagtaat tgcatatgca attgatagta aaacacacat
    18601 taattctaca tttctagaca ctatagtaat gcaccataaa ataaataatt ctataatagc
    18661 aaactgtcct gtaaatgcca tagctgattt ttcatcttgt acataccttt catctgcttt
    18721 atcaccgttt atttttgcta taataaagtt tgagaaaaaa gcaaaaaagc caatatatgt
    18781 aaggtcagca atattaccag atgaaaaata tctaagactt aaaaatccaa tgaaaccaca
    18841 aaaacccata aaaaattttt tctgaatcat ttcttatcct ccttagtatt ttaaaaagag
    18901 atatatttgt atctttatga tataaatata tctctttttt atttttttgt caatataaaa
    18961 gcagtgttat acaatatttg taatgtacca taattactag accaaaaatc taatgattga
    19021 ggtgtttatg tatgactaaa tacgatttta aatttaaatt aaaagtagct aaattttatt
    19081 taaatagaga agaaggagaa gttatgtcat tcagtgtctg atatatggct aactttaaat
    19141 caatagtgat gttttaaata aagctatcaa tattacaaag gattgtcctt atagaaggac
    19201 ttttaactca aaccaaggtt ggccatataa aatgaataag tataacaaaa cacttaagaa
    19261 aaataaaata tttcaaggta tatctcgtaa ggctacattc tttgataact ctcctatgaa
    19321 aaattcattt ggagtaatga agcgagaatg tattataaaa aaatataaac aattatttga
    19381 aagttttttt gtaataataa agaggattat gagggggcta agaaacttag atatgaaggt
    19441 attgctaagt taattgtaga aggtgtatta aataaaaata taaataatga gggagttaaa
    19501 cagatgtaca aacatacaat agtttatgat ggtgaagttg ataaaatccc tgcaactgtg
    19561 cttggttggg gttatggttc aaataaaatt ttaatatgta atataaaaga ttatgtacca
    19621 ggaagaactg aaaatcttta tgttgttgtt ggtggagcat gtgagaagat aggttctata
    19681 actaaagaaa actataccat gataaagggt agtgatagat ttgatacact ttacaaagta
    19741 ttagatttta taaatagata gattaaaagg tagcaactag taggttgtta ccttcctttt
    19801 tctagctttt tatatctgac aatttttgtt atttgtataa ctataaagtt aatataagaa
    19861 gatatttgta agtaagagta
//
LOCUS       DAMJVM010000064        19410 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.067, whole genome shotgun sequence.
ACCESSION   DAMJVM010000064 DAMJVM010000000
VERSION     DAMJVM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19410
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.067"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            41..430
                     /locus_tag="PC523_002440"
                     /pseudo
     CDS             41..430
                     /locus_tag="PC523_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
     gene            453..977
                     /locus_tag="PC523_002441"
     CDS             453..977
                     /locus_tag="PC523_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2475953.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIV
                     WANGKNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFVVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            1077..1895
                     /locus_tag="PC523_002442"
     CDS             1077..1895
                     /locus_tag="PC523_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2475954.1"
                     /translation="MLSVTRYSIKNIRLKPWVKFIWFLETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKFENQDYTASNMHFNGIRDKHGFIVQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSKLFAKKLEESLNPTKAIEKVVLCLEEVLLSILEEDLISNKSV
                     ELLNSFIYVNRYSNIKAFCDDTNINIKTLERTCLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFIGENKTIKENIKYSYL"
     gene            1973..2284
                     /locus_tag="PC523_002443"
     CDS             1973..2284
                     /locus_tag="PC523_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDF2475955.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKSLKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(2461..3144)
                     /locus_tag="PC523_002444"
     CDS             complement(2461..3144)
                     /locus_tag="PC523_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475956.1"
                     /translation="MQKEVAMNKLIKDCVNFFNDCGFSYSICGGYALELFANMHIRPH
                     SDIDIWISDTDKEKSIQYMLNQGWDIYEPLGNYLLRPILDLNKQHIEQICVFAIKPDC
                     SFIELKKVEDDNYKMNIQCDEQLQFDFIELIYNPQKDGKFLFSQNQRIIRDMDKARLI
                     TSDNIPYMAPEVVLFCKSIYIDRVGYQKDFDVTVPLLSQEQRDWLVKALITAYPDGHA
                     WIDQLANQH"
     gene            complement(3237..3878)
                     /locus_tag="PC523_002445"
     CDS             complement(3237..3878)
                     /locus_tag="PC523_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2475957.1"
                     /translation="MGRKKEPDNTIQKILDISHKLFLEKGYEQTTIQDIVDNLDGLSR
                     GAIYHHFKSKDEIIKAITSRLYNDGFYGYDVKNDEGKTALEKLQYRLLHAYEQHNKQD
                     IFLMSSSLLRNPKYLATQFDEIINDIVPWIKALIEEGNQDNSMQVKNPEIAAQMFGFL
                     LNTWCIPTVYPGNPQQMVERFTLFGEQAAFMGISFMNDKIMCEYQKFVNKFSK"
     gene            complement(4052..6055)
                     /locus_tag="PC523_002446"
     CDS             complement(4052..6055)
                     /locus_tag="PC523_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HDF2475958.1"
                     /translation="MKSQKRFKILDIDIYLQPFESDIKKRMKHYEYMKSKILKNCKDF
                     SSFANAHLYYGFHKTNDGWVYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTTNGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTSFIWTDN
                     DFDLKNIISPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSVNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLSMGVPDFWIKTISNLSDENWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNPVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYIKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDI
                     VEFGGNTKKENLRDYDVYFSSTPPSSKIPIASRTAIVLLDNGI"
     gene            complement(6208..6846)
                     /gene="recX"
                     /locus_tag="PC523_002447"
     CDS             complement(6208..6846)
                     /gene="recX"
                     /locus_tag="PC523_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDF2475959.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEDELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENTIDKVIDFLKS
                     YNLINDSILAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTIDEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
     gene            7122..7811
                     /locus_tag="PC523_002448"
     CDS             7122..7811
                     /locus_tag="PC523_002448"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDF2475960.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
     gene            7812..8384
                     /gene="yqeK"
                     /locus_tag="PC523_002449"
     CDS             7812..8384
                     /gene="yqeK"
                     /locus_tag="PC523_002449"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDF2475961.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDALRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            8501..8851
                     /gene="rsfS"
                     /locus_tag="PC523_002450"
     CDS             8501..8851
                     /gene="rsfS"
                     /locus_tag="PC523_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDF2475962.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            9205..11625
                     /locus_tag="PC523_002451"
     CDS             9205..11625
                     /locus_tag="PC523_002451"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDF2475963.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            11966..13027
                     /locus_tag="PC523_002452"
     CDS             11966..13027
                     /locus_tag="PC523_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475964.1"
                     /translation="MYQRIIFPMLKREVNIPGNLNYQYKYNGVKESKSVKTSWEFKDE
                     DKRKFRLMCLPNSKTVSAIINDLIYFHSTEEYKLQFIGMRMNVLVDIWAHMYFIGKPE
                     WYINDIKEFIAEESKYIAENKWTKSEEYRNGWHAINVSGTLDACGYESISYLGYGRMG
                     NVPDYGYLNYRYVPNWSCTDDKVKIQKNNQDNFFKAFCQMFYALKCIKNGNDFNINQY
                     DNLTAQQKTEVTKVIATRKTDQSDAWEKVIKVLGYSSLEKFDKNKWKNEFEKFSNKKK
                     INYYYFNLVAKKHIDYVNNFLEENRLKLDGMCSEYCELKVFDDTQFAKNKNRVIKIVL
                     RGDFIVDAIQLVYDGKCKT"
     gene            complement(13756..14316)
                     /locus_tag="PC523_002453"
     CDS             complement(13756..14316)
                     /locus_tag="PC523_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475965.1"
                     /translation="MKLRNPKIKKLLSVALLAPLVGIPIGQTANAMEISNDIPSYIEN
                     NPITIEINDSQLPKPGTVTYIDMNKLIKNLKENGIDTTIIENELYYRKAGTNKIVWTK
                     MGFDLYLSKGVALTLAGAGTGVAGILAGAIPGIGQYLAAAIVGAITMHLGSNITSGKI
                     FKFKKMRYNKGGTYVTKYYLQSVRNQ"
     gene            14754..15425
                     /locus_tag="PC523_002454"
     CDS             14754..15425
                     /locus_tag="PC523_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475966.1"
                     /translation="MKKYIRYQSRSELKQLRKLFEYKIDDIVGYLFCSRRTFERIERD
                     NGTSNICLANRIAELYFQRFDELFIEVDLEVIKFINSIREYTLSIEDSYIIENEIYYC
                     IFLKKLDTCRDQIWGSTYWLEDYNKYKDIRKLREANVAYLLKNTNHKITIINTENQWY
                     RIYFGLCIGKFHEILISKSCAEKCVPYILKRHVVESSLLFNNSKISDLMLLGKYDDKR
                     NILID"
     gene            15620..15814
                     /locus_tag="PC523_002455"
     CDS             15620..15814
                     /locus_tag="PC523_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475967.1"
                     /translation="MKGGEYMKTLMFVFVIMYYITIVIKSITTIVKSITRLLDSISRL
                     KESLHKLRNRPDGNQDDFNK"
     gene            16317..16406
                     /locus_tag="PC523_002456"
     CDS             16317..16406
                     /locus_tag="PC523_002456"
                     /inference="COORDINATES: protein motif:HMM:NF024835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2475968.1"
                     /translation="MPPMATMKKICLALDCNIEDIVEIKKDED"
     gene            complement(16593..17588)
                     /locus_tag="PC523_002457"
     CDS             complement(16593..17588)
                     /locus_tag="PC523_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDF2475969.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTNNLDEIKDFFEKGIYC"
     gene            complement(17709..19337)
                     /gene="aspD"
                     /locus_tag="PC523_002458"
     CDS             complement(17709..19337)
                     /gene="aspD"
                     /locus_tag="PC523_002458"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HDF2475970.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAINETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFIHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKNASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
BASE COUNT         7247 a         2488 c         2987 g         6688 t
ORIGIN      
        1 tgaagcagga accctgtgac tttagtcatg ggaggttcag aaagcacttg ataatatatt
       61 agaagaggga atagaaaatg tattaaatag acatgaaaag atagcttata gtgttagaaa
      121 atctattgag gaatatggtt tagaactatt tttagaagaa ggatattcta acacagttac
      181 agctataaaa attcctgaaa gcataggagc tttaaagctt actgattaca tgcttaaaaa
      241 ttataataca cttgttgcga catcactaaa tcaatatatg gatactattt taagaatagg
      301 tcatatggga gaaaacgcaa atctaaacaa aatagagcac gtactaaatg ttttggataa
      361 aagtttaagt gcattaggat ttaaagaaaa tggaactttg ttaaacttat ttaataagta
      421 ttatttttaa taaataaaca gggggagata gtatggaagt tacagctaag caaaaattta
      481 caacgaaaga catggtggaa acctcactat taatagcatt agtatttatt gcaactaaat
      541 ttataaatat aagattgcca atatctataa atggtgggct tgtacattta ggaactgcaa
      601 tgttgttcat atcagcaata gtatttggaa gtaaaaaagg tgctttatca ggagctattg
      661 gtatgtcact ttttgattta atatctgggt ggactttgtg ggcaccattt acctttatag
      721 ttagaggcgt tatgggttat ttacttggaa agattgtatg ggcaaatggt aagaacggga
      781 ataatttttt aatcaatgta ataggaatat gtgtttcatc aatttggatg ttatttggat
      841 attatgtaac agaagttata ctttatggta attttgtagt acctcttact tcaataccgg
      901 gtaacttaat gcaagtattg ataggtctta taatagcgct tcctatttct aaagtattaa
      961 aaaaatgtat aaaataaata taaagtgaac taaaatttac gatagcataa attatactgt
     1021 ataatattaa gatggagtac ataattaaaa aattttagta tagaggttag ttcacaatgt
     1081 tatcagttac aagatattca atcaaaaata ttcgtttaaa gccgtgggtg aagtttattt
     1141 ggtttcttga gacaaaatca aatatactgt taaataataa gttacttccg acagacagta
     1201 tagatattat tcttaatctt tctgatgtta tggaatataa atttgaaaat caagactata
     1261 ctgctagtaa tatgcatttt aatggaataa gggataaaca tggatttatt gttcaacatg
     1321 gaaatatacg tgttataggg atttcttttt atccatttgg tctatatcca tttttaaaga
     1381 taccgatttc agagtttaac agacaaattg ttgacttaga agcagtatca aaactatttg
     1441 caaagaaatt agaagagtca ttaaatccaa caaaagcaat agaaaaagtt gttttatgtt
     1501 tagaagaagt attattatcc atattagaag aagatttaat ttcaaataaa tctgtagagc
     1561 ttttaaattc atttatatat gttaatagat atagcaatat aaaagcattt tgtgatgata
     1621 caaatataaa tattaaaaca ttggaaagaa cctgtttaaa atatacaggg tatacaccta
     1681 agattttaaa gagaatttac agatttaaaa tggcaagtaa tcaacttatt tataattata
     1741 aagatgatga actttttgat tttatttatg aaaatgagta ctatgaccaa gcgcacttta
     1801 taaaagaatt taaaaaattt tcagggacgt caccaattaa atttattggt gagaataaga
     1861 cgattaaaga aaatataaag tacagctatt tataattata ctgtcgattt tttacaatac
     1921 ctccaattta ttttttgata tgatatagga aaataaattg gaggttttat atatgaaaat
     1981 tattacagaa atagttgagt ttaatgtgga agaaaatttg tcaaaagaag agtttataga
     2041 aattgtaaat gacttagaat taaaatttca ttctattcaa tccggtttta ttgatactga
     2101 gcttttatat gatgaaaagt ctcaaaagtg gattatgata caacattggg aatctataga
     2161 agacctcaag agtgcttcta gtaaaatgtt taaagattct tcaactgaga aatttagaaa
     2221 atctttaaag aatcaaactg ttaagatgaa tataatacca caaattaaaa gttggaaatt
     2281 atagaactga atgtaaaaga tagttaaata gtatcaaact aaattagcca aataacattt
     2341 tgtatttaaa ttgttatttg gcttttagtg tttattttaa tatgagtgtt atatactaga
     2401 aaaaccaaca aactgttaga gtgtggatta tcataatgtc atattcggga gctaaaaggc
     2461 ttaatgttga tttgccaatt ggtcaatcca tgcatgtcca tctggatatg ctgttattaa
     2521 agcctttaca agccaatccc tttgctcttg tgataatagt gggactgtta catcgaagtc
     2581 tttttgataa ccaactctat ctatgtatat tgatttgcaa aacaaaacca cttcgggagc
     2641 catatatgga atattatctg atgttatcag tctggcctta tccatatctc ttataattct
     2701 ttgattttgg gaaaaaagaa attttccatc tttctgtgga ttatatatca attcaataaa
     2761 atcgaactgt aactgttcgt cacactgtat attcatttta tagttatcat cctcgacttt
     2821 ttttaattca ataaatgagc aatcaggctt aatcgcgaaa acacaaattt gctctatatg
     2881 ttgcttgtta aggtcgagaa taggtcgtaa taaataatta cccaagggct catatatatc
     2941 ccatccttga ttaagcatat actgaatgct cttttcttta tctgtgtcag aaatccatat
     3001 gtcaatatca ctgtgtgggc gaatatgcat attcgcaaat aattctaggg catatccacc
     3061 acatattgaa tatgaaaagc cacaatcatt aaaaaagttt acacaatctt taattagttt
     3121 gttcatagca acctcctttt gcatgtaccg aaataaatca aatttttaaa aataccaact
     3181 aaatgtttgt tttgtgtaaa tataaaactg ccaatattaa ggcagtttta tatttactat
     3241 ttagaaaatt tattaacaaa tttttgatat tcacacatta tcttatcatt cataaaagaa
     3301 atacccataa aagcagcttg ctcaccaaac aaagtaaaac gttctaccat ctgttgtgga
     3361 ttacctggat aaactgttgg aatacaccat gtgtttagaa gaaaaccaaa catttgagca
     3421 gcaatctctg gatttttaac ttgcatagaa ttatcttgat ttccttcttc aatcaatgct
     3481 tttatccaag gcacaatatc atttataatc tcatcaaact gcgtagctaa atacttagga
     3541 ttacgcaata gtgatgatga cattaaaaaa atgtcttgtt tattgtgttg ttcataagca
     3601 tgaagcaacc tatattgtaa tttttctaat gcggtctttc cttcatcatt ttttacatca
     3661 tatccataaa atccatcatt atacaaacga cttgtgattg ccttaattat ttcgtctttt
     3721 gatttaaagt gatgataaat agcacctctt gaaaggccat ctaaattgtc tacaatatct
     3781 tgaatcgttg tctgctcata ccctttttct aagaacaact tatgtgaaat atcaagtatt
     3841 ttttgtatag tattatcagg ttcttttttt cttcccaaaa agctcactcc tcaaaacaat
     3901 catttggtat gtttatctta gcaaatacat aatgatttgt caatgtgtat ttttagcatg
     3961 ttgaacaggt atattgccat gctttcatta ttttattttt ttgccttata ctggattata
     4021 tttgcagttt cctaatattt ttggctcata tttaaatacc attatctaaa agcactattg
     4081 ctgtacgaga ggcaataggt attttagaag aaggtggagt tgagctaaag tacacatcat
     4141 aatctcttaa gttttctttt tttgtgtttc ctccaaattc tactatatca gtatttaata
     4201 tcgttttgta gtttgtattt tgaaactttg aaatatcaac tgattgtatt agattagggt
     4261 ggaaattaaa tataaataaa tatttgccct taatgtacac caaaatcttt ttatcattat
     4321 ccatatgtat aagttcatga ctattttgga taaaaatatc actgtatttt gtaagttcaa
     4381 gcatagattt atcaaaattt aataattgtt tatactttaa gttgttatct tctgaaaggc
     4441 tccattgtct tctagcatac ttatagctcc aattgttgcc ttctcgtgga aaatcaatcc
     4501 actcaggatg tccaaattca tttcccataa aatttagata cccttcacct gctagagaaa
     4561 aagttattaa tttaattaat ttaataagag atattgctct attgataaca gggttatttg
     4621 agtttatttc catattccag tacatttcct tgtcagctag ccaaaaaata attgttttat
     4681 cgccaactaa tgcctggtca tgagattcac aatatcctat atttttttct ccaggtcgtc
     4741 tagtagtaag ttcataccac attttgccta aatcccagtt ctcatcagat aagttagaga
     4801 ttgtttttat ccaaaaatca ggtactccca ttgaaagtct atagtcaaat ccaattccgc
     4861 catcttttat agggatacac ataccaggca ttccactcat atcttctgcg atacttatcg
     4921 aattaggttt aatttctttt attaattcat ttgcaaattg gagatatgtt atagcttcaa
     4981 tatctgtatt cacactaaaa tatttttcat aactatcaaa tgaaactcca agaccatggt
     5041 tatgatataa cattgaagtt acaccatcaa atctaaagcc atcaaaatgg tactcattta
     5101 accaaaattt tatatttgaa agcaaaaaat gtattacttc aggtttacca tagttaaaaa
     5161 gttttgtacc ccaagcagga tgatttccct ttgaacctga gtggaaaaat tgatgttcac
     5221 ttccatcaaa ctcatttatt ccttctaaag tattctttac agcatgagaa tgaactaaat
     5281 ctaataaaac agcaatcccc attgaatgag cagtatttat gagatttttt aagtcttcag
     5341 gagtaccaaa tctagatgag attgcataaa agtttgaaac ttggtatcca aaagatgcat
     5401 aatatggatg ttccataata gccataagct gaatggtatt gtatccagct ttttttatct
     5461 taggaagtat tttttcagta aattcattat aagttccaat agattcgctt tcagtagaca
     5521 tacctatatg acattcatat attagtggag aaattatgtt ttttaagtca aaatcattgt
     5581 cagtccatat aaatgaagtt tttggttgcc atatttgtcc attaaatcca ccaaaatctt
     5641 tttgaattac tctttttata tataatggta tacggtcaaa tgtttttcca tttgtagtaa
     5701 cttgtacctt tacctctgat ttgtgaggta agctattttg accaggaata aatatttccc
     5761 aattaccaga accaattttt cttaggggat gagattttct attccaatta ttaaagtcac
     5821 caattagtga taaactatct gcatttggag cccattccct atagacccat ccatcatttg
     5881 tcttatgaaa tccatagtaa agatgagcat ttgcaaaaga actaaaatct ttacagtttt
     5941 tcaatatttt tgatttcata tattcataat gtttcattcg cttttttata tcagattcaa
     6001 atggttgtag gtatatgtca atatctaaaa ttttaaatct tttttgactt ttcattgtat
     6061 ttctcctcca tagtaaatat tacatctaaa aaataatatt tatctcatac tatcttctct
     6121 atataattga tatatattat ataatcccaa taaaaagctt aagccaccaa tgattaattt
     6181 cataatttac ctccctagta tctatactta tatatcgtat tcatcaaaat ttaaaagttt
     6241 gtttaaaact cttttaataa tatcataatc aaagccttta taggaaagat gttgagaaat
     6301 tttttgatat attttctttt tatcttcctt cttcactctt tcataccttt tttttgctag
     6361 atacatagca ttttcaaatt cagtgtcctt atctaattca gatactgctt catcaattgt
     6421 acttctattg atacctttat tatataaatt ctgcttaatt ctgttttttc cgcatttatt
     6481 tagattaaca tttgtgttaa ctattttttg agctaatata ctgtcattta tgagattata
     6541 acttttaaga aaatctatga ccttatcaat tgtattttca tcaaattctg atgacagttt
     6601 ttcttttaat tttttttcag actggtcagc atgagataaa atatttagtg ctttattttt
     6661 agcttttata tacatttcat catctaaaat aggttttaat tcatcttcat ttatttcatt
     6721 tccttttttt aaattaaatg tataaacaag ttctttaaat atagcaataa aaaatttatc
     6781 atccacataa atattaactc tatcatcatt tcttttttgt tgttctattt ttgtaattat
     6841 acccataaat cctccttttt tatatgtaat tatattaaca aaagcaacta ataaaattta
     6901 ataaaaaact tatatttgta tgttatattt tatcaaaatt aagacaaaat agtaaaaagt
     6961 tatttttatt aaaaagtaaa gtggaatcat gttaaataat tattttttaa agcgtttttt
     7021 agcatataaa tataattata taccaaaaaa aacaaattat aagctttaag aaataaaaaa
     7081 taatggtaca atagtattat aacattaaaa ggagacaaaa tatgagaagc gaaaatctag
     7141 ttaagatggc agagttgaaa aatactgaaa aactagagaa atttaatcgc cataaaggca
     7201 aaatcaaaat aggtatattg ggaggtacat ttgaccctat acactatgct catttagcaa
     7261 cagcagaatt tattagagac aagtatgaca ttgacaaaat aatctttata ccatcaggaa
     7321 atccaccaca taaattatgt atcactacag ataagtatga tagatataat atgacacttc
     7381 tagcaactga aagcaacgaa gattttttag tttcaaaagt agaaatcgaa agaaagaaaa
     7441 ggacttatac aatagataca ttaaaatact taaagaaaaa atacaaaaat gcagacattt
     7501 attttataac aggtgcagat gcgatttgta gtgttgaaga gtggaaagat gtaaagaaaa
     7561 actttgagtt agctactttt atagcagcaa ctagaccagg aattagtttg ttaaggtcac
     7621 aagagactat agaaaaacta actaaaaagt ataatgctga cattataaca gtttatgttc
     7681 catcactgga tatatcgtcc acatatataa gggaacagct aaatgaaggc aagtcaatac
     7741 gttacttagt acccgaaaat gtagaaaatt atttatatga aaataaacta tatcaatatg
     7801 gagatgatta aatgaactta gaaaatatta accagaggct aaatcaaatg ctaccagtgg
     7861 ggaggcttag ccattcaaaa aatgttgcaa aatgtgctga aaaattatgt gagatttatg
     7921 gatgtgacaa agagaaagct tacttagcag gaatgattca tgattgtgca aagtatttaa
     7981 gtgacaagga aatagaggat tatgtaaata agtatgaaat atatttagac ccaatagagg
     8041 atggaaaccg ttccttgtct catagtgtaa ttggggcata catatgcgag tatgagtttg
     8101 aagtagaaga tgaagatatt ataaatgcaa tcaaatatca tacaacaggg agagaagata
     8161 tgtctctttt agaaaaaata atttacatag ctgatttgat tgaagaagga agaaaattcc
     8221 cagttgtaga tgcattaaga gaattagcat atggaggtaa acttgatgaa gcacttttaa
     8281 cttcttttaa taatacttta atgtttgtca tcaataaaaa agaagagata catccaagaa
     8341 cagttatggc aagaaactat ctaattaaag aaaagttatt ataaaaaacc tatataaagg
     8401 taaacatatt aataaattta aagttgacta tgttacaaaa tcgtattaat atgttacaat
     8461 aactaagtgt attttaatta ggaaagtgag gaatttttat atgacagtgg aacaaatgac
     8521 aaaaatagca tatgatgcca tagaagataa acttggtcaa gatacagtta taataaatat
     8581 aggaaaagtt tctagtttgt gtgattattt tattattact acagcatctt ctcaaagaca
     8641 ggtaaaagct attgctgaca atgtggaaga tgaattggca aaactaggat tagagccaag
     8701 aggtaaggaa ggacaaggga ctcaaacttg ggtgctcctt gattatggag acataatggt
     8761 tcatgtattt aatgaggaaa atagagggtt ttataattta gaaaaattat ggaaagatgc
     8821 accttatata gatattgaca cattagcata aacatgataa tatatgttat tataattaga
     8881 aaatttcaaa aattatatta aattgaatag actagagcag taaaagatta tattttttca
     8941 gagagttagt ggaaggtgag aactaatatt atataacttt gaacttacta gattaaaatc
     9001 tattctccat ggctggatta aaagctatct aagagggtct atgtatatag tagactaatt
     9061 agggtggtac cgcgaagatt tatcctcgtc cctaaacgta agtttagtga cgaggatttt
     9121 ttattttcaa agaattattt ggaaattata agtaaataat actaaacaag gtagttatac
     9181 acaactaaaa ataggaggta aaatatgagc gtatataatt ttaaagaagt agaatcgaaa
     9241 tggcaaaaaa tttggaaaga caataaccag tataaaatgg atactgcaca aactgaaaaa
     9301 cctaattatt atacattaga aatgttccct tatccatctg gaaaaataca catgggacat
     9361 gttagaaatt attctatagg tgatgttgtc gcaagattca aaaaaatgga aggttataat
     9421 gtacttcatc caatgggatg ggattctttt ggattaccag ctgaaaatgc agctataaaa
     9481 catggaatac atccacataa atggacaatg gaaaatatag aagaaatgaa agagcaatta
     9541 aatttattag gtattagtta cgattgggac aaagaagttg caacttcaac tccagaatac
     9601 tatagattta cacaagaaat atttttgaag tttttagagc atgggcttgc atataagaaa
     9661 aaatcatatg ttaactggtg tccttcttgt gagactgttc ttgcaaatga gcaagttgtt
     9721 caaggagcat gtgagagatg taaagcaaca gtattaaaga aagacttaga acaatggtat
     9781 tttaagacta ctgaatttgc agaagaattg cttaatgact tagatacatt agatggatgg
     9841 ccagaaaagg ttaaaacaat gcaaaaaaat tggataggta aaagtacagg agcagattta
     9901 gtttttgata tagatggaac tgataaatct atgacagtat ttacaactag accagataca
     9961 acttatggag ttacttatat ggtacttgct ccagagcatg aattagttaa agagttggtt
    10021 gcaggtactg aatatgaagc agatgttgaa gcttttgttc aaaaaatgca tactatgaca
    10081 gaaatagaaa gaacagcagc tgatgtagaa aaagaaggaa tgtttatagg tagatatgtt
    10141 ataaatcctt taaatggtaa gaaagttcct ttatggatag ctaattatgt attagttgag
    10201 tatggaacag gtgccataat ggcagttcca gcgcatgatg aaagagatag agattttgct
    10261 gaaaaataca atttagatat aattgatgta ataacagaag ataacaaaat gataaattcg
    10321 gaagagttta atggattaga tgcttcagaa ggatttgaag gaataataga taagttagaa
    10381 aaagaaggta gaggaaagag aacaattaat tatagattaa gagactggtt agtttcaaga
    10441 caaagatact ggggttgtcc tatacctgta gtttattgtg atgaatgtgg aatagttcca
    10501 gttaaaaagg aagatttgcc agttctttta ccaacagatg ttgaatttac tggaaaaggt
    10561 gaatctccac ttactacttc aaagcaattt atgtcaacaa cttgtcctca ctgtggaaaa
    10621 ccagctagaa gagaagttga tactatggat acatttgtag attcttcttg gtatttctta
    10681 agatatgtgg atagtaataa tgaaaatgaa ccatttagca aagaattagt taatagatgg
    10741 catccagtag accaatatat aggtggagta gaacatgcta taatgcactt actttatgca
    10801 agatggtttg taaaggcatt taagagtatg gggatggtag acttcaatga gccatttaaa
    10861 aacttactta ctcaaggaat ggttcttatg gatggttcta aaatgagtaa atctaaaggg
    10921 aacacagtat ctcctatgga tataatagat gagtatgggg cagatactgc aagacttttc
    10981 gtattatttg cagcaccacc agaaagagat ttagattggt cagagcaagg tgttgatgga
    11041 tgctttagat tcttaaatag agtttataga ttggttgacg agttggcaga cgtggttaaa
    11101 aaagatgtag aatttggaga attaaattct caagataaag atatgagata tactatacac
    11161 tctacactta aaaaagttac tgctgattta agtgaaaaat ttggatttaa tactgctata
    11221 tcagctctta tggaattaat aaatgatatg tataaatata aagagttaga taatataaac
    11281 gaagcagtta taaaagaagg tgtacaaact atagtaacta taattgcacc atttgcacct
    11341 catttaggtg aagaactatg gactatgata ggtaaagaag gaagtgtatt tgacatagac
    11401 tggcctaaat atgacgaaaa agctctagta aaagatgaaa tagaggttgt tgtacaagtt
    11461 aatggaaaag ttagaggaaa attaactgtt aactcaaata tatctaaaga tgagatggaa
    11521 aaggtagctt tagaagatga aaaaataaaa ggattagttg aaggtaaaac tatagttaaa
    11581 gttgttgctg tacctaagaa gttagttaat atagttgtta aataacttaa aaatataaat
    11641 ttataaaata aaggaggctg tctcaaaata gagataagcc ttttttcatt gttgaaatta
    11701 taaaaataga tttgatttca tacaagaaaa gttcctaatg aactaaaaag ttcattctga
    11761 gacaccctca ttttcatctt gaaaattatt acaattaatg ttaaatatcg tgaatacatt
    11821 gagagagtat ttgctgtgac ttctgtagat attaaagtat aagaagaaat aaaagaatat
    11881 taaagtaact agaagaatat aaagcgtagg taaaatatgt aagaattata tgctattgta
    11941 acttacaatt tatagagtgg agtttatgta tcaaagaatt atcttcccaa tgctaaaaag
    12001 ggaggtgaat atacctggaa atttaaacta tcaatataaa tataatggag ttaaagagag
    12061 taaaagtgta aaaacaagct gggaatttaa agatgaagat aagagaaagt ttaggttgat
    12121 gtgtttacca aatagtaaaa ctgtttctgc cattataaat gaccttattt attttcatag
    12181 tactgaagaa tataagttac aatttattgg aatgagaatg aatgtacttg tagatatctg
    12241 ggcacacatg tattttattg gaaaaccaga atggtatatt aatgacatta aagaatttat
    12301 agcggaagaa agtaaataca tagcggaaaa taaatggact aaatcagaag aatatcgtaa
    12361 tggttggcat gctattaatg tttctggtac tctcgatgct tgtggatatg agagcatctc
    12421 atatcttgga tatggaagaa tggggaatgt accagactat ggatatctta attacagata
    12481 tgttccaaat tggagttgta ctgatgataa agtgaagata caaaaaaata atcaagataa
    12541 tttctttaaa gcattttgcc aaatgtttta tgcattaaaa tgtattaaaa atggaaatga
    12601 ttttaatata aatcaatatg ataatctaac agctcaacaa aaaacagaag ttacaaaagt
    12661 aatagcaaca agaaaaactg accaatcaga tgcatgggaa aaagtaataa aagttttagg
    12721 atatagttct ttagaaaaat ttgataaaaa taaatggaaa aatgaatttg aaaaattttc
    12781 taataagaaa aaaataaact attattattt taatttagta gccaagaagc atatagatta
    12841 tgtaaataat tttttagaag aaaatagatt gaaactagat ggaatgtgta gtgaatattg
    12901 tgaattaaag gtttttgatg atacccaatt tgctaaaaat aaaaatagag taataaaaat
    12961 tgtacttaga ggagatttta tagttgatgc tatacagctt gtatatgatg gaaaatgtaa
    13021 aacttaacta catggagaac cttttggagg tatcatagtg gatatggatt tagatgcgga
    13081 tgattatata attaaaataa gtggctctat agggtggttt acctatacca gctagaacaa
    13141 taggtaaaat aactttttta actaaaaaag gaaaaactat agcaacagga aaagaacgag
    13201 catttaagaa atatggaaac tttactctag agccagaacc aggtaaacaa atatttgccc
    13261 ttcagggttc ctactcaata tataaattta gttcatagta tagcaaaaga tatttagatt
    13321 ctctagggat tgcttgtatg aaataattat ataaaaaaat atttaaaatt caaattagaa
    13381 gtttttgttt aatagatata ttagaaggta gcaactagtt agttgttact ttccttttta
    13441 atttaatcag tttttacagc gtttataata taacaaccaa ttcatatatt agatttactt
    13501 ttccgaataa aagtatggca gtataacaag tatctaaaat attacaaata ttaaatgaga
    13561 aaaagatggt aagttaactc caccatctta ttttttatct attaatgaat gatgctacaa
    13621 aagtcagtat aaaaaatgaa acaaacaata taatgaactt gtgtaattta cttagttgaa
    13681 acactttatt agaaacgaat aaaattaatg ctgatacaat tgcaaaaagt agatatttta
    13741 taatcatttt atcacctatt gatttcttac agattgtaag tagtatttgg ttacataagt
    13801 cccaccttta ttataacgca tttttttaaa tttaaagatt tttccagatg ttatatttga
    13861 cccaagatgc attgtaattg ctccaactat agcagcggca agatactgtc ctataccagg
    13921 aatagcacca gccaaaatac ctgcaacccc tgtaccagca ccagctaaag ttaatgcaac
    13981 tcctttagaa agatataaat caaagcccat cttagtccaa actattttat ttgttccagc
    14041 ttttctataa tataattcat tttcaataat agtagtatca ataccgtttt ccttaaggtt
    14101 tttaattagt ttattcatat ctatgtatgt aactgtgcct ggtttaggta attgagagtc
    14161 attgatttct attgttattg gattattttc tatataactt gggatgtcat ttgaaatttc
    14221 cattgcattg gctgtttgcc cgataggtat acctacaaga ggtgctaata atgctactga
    14281 taacaatttc ttaatctttg gatttcttag tttcataaaa tacctccatt ttaaattagt
    14341 tatatatcaa agatatatat tattattgta gaatatagtt attaataatt aactatatta
    14401 aatattaata aaaaatatag taaaacttaa gaatacatac aataaaactt aatataaatt
    14461 aagtctatga tataaagatg gaagtgtact atcaatatat ctttgctaaa tcaactgtta
    14521 tcaaagagag tattcttcat tttgaaaggt gattttcaaa tataagaagt aataatattt
    14581 atattcttaa aaaagtaata tatttttaaa gtttttagca acaggggacc ttttagaaaa
    14641 aagctgcaag agttgataat gctaatgttc atgtataaaa tctacaaaaa taaaatgtcg
    14701 tagtaaaaaa agaataaaac tattaaacta aaatcaggag gtgataataa aatatgaaaa
    14761 agtatataag atatcaaagt cgaagtgaac ttaaacaact tcgtaagtta tttgaatata
    14821 agattgatga tattgtagga tatttatttt gtagtagaag aacatttgaa agaatcgaga
    14881 gagataatgg aactagtaat atatgtttag ccaatagaat tgctgaattg tattttcaaa
    14941 gatttgatga actgtttatt gaggttgact tggaggttat taaatttatt aattctattc
    15001 gagaatatac tttaagtatt gaagatagtt atattataga aaatgaaata tactattgta
    15061 tttttctaaa aaaattagat acatgtagag accaaatatg gggaagtaca tattggttag
    15121 aagattataa taaatacaaa gatattcgca aattaagaga agcgaatgta gcttatttac
    15181 ttaaaaatac taatcacaaa ataacaatta taaatacaga aaatcagtgg tatagaatat
    15241 attttggctt atgtattggt aaatttcatg aaattcttat ttctaaaagt tgtgcagaga
    15301 aatgtgtgcc gtatatatta aaaagacatg tagttgaatc aagtttattg tttaataact
    15361 ctaaaatatc agaccttatg ttattaggta aatatgatga taaacgaaat attttgatag
    15421 actaaacatt acattttata aataaaacgg taatatttat tttttggaat caaggatata
    15481 aaaaatatat aaattaatta agaataaaca agaataaata tttggtaatt ataaaattat
    15541 gttataatta aaattcaaga ataatcttgc aagagactac aatctaaggg cgaaagtttt
    15601 caggtacaat caccttcgta tgaaaggggg tgagtatatg aaaacactta tgtttgtatt
    15661 tgttataatg tattatatta ctattgtaat aaaatctatt acaacaatag taaagtctat
    15721 aacaagatta ttggattcta taagtagatt aaaagaatct ttacataaac taagaaatcg
    15781 tcctgacggc aatcaagacg atttcaataa ataaatcatt acttaaatta ttttgaattt
    15841 gaaacttcgc tctaggctaa tagattgtag tttctttttt tgtttttgat actatttcat
    15901 acctttattg tatatcaaat cgtcaatgat gacaatactg acaacattga caattttata
    15961 tagttgtaaa ttatttttat atggaataaa ttaggaaaat ataatttgaa tattctaatt
    16021 tattataaaa aatatttgtt ataatattac atatgattta gtatgtaaga atttagaaaa
    16081 aaaggtgcag actatgaaaa ttagtaaatt accagaagca gaattaaaag ttataagata
    16141 tatttaggaa tcagacaagg tattaacatc aagagagatt gttgaaacta tggagcagga
    16201 tgataagaaa gtagattaaa aaaattaaat gaattagaag catattttaa gagtttggga
    16261 caatatgatt aataaaaaat atagtactat ttagtaaaaa atggagtatc aattttttgc
    16321 cacctatggc aacaatgaaa aagatatgtt tagcgttaga ctgtaacata gaggatattg
    16381 ttgagattaa aaaagatgaa gattaataag taaaaggtag ctactagtat aagtgctacc
    16441 tttttttgtg ggtaatattg tggataagtt gtgggaaact tatataaaaa ttgtggataa
    16501 aatattttac tgacataaat attggacttt gatttttaaa tgttgaaata tcaatgaaaa
    16561 aatacataaa atcataaaag atattatttt aattagcaat agattccttt ttcaaagaag
    16621 tctttgattt catcaaggtt attagttttt ccaagtgcta agataagttt tattctagct
    16681 ttttgacctg gaaggtctcc accaaatata cagccaagat tttttaaatc tctaccagaa
    16741 ccaaagtaac cataactatc gaatactcta cctgaatgac acctagaaac tataacaaca
    16801 ggaagccctt tttctctagc atattcaact ccagaaagca tttgaggagg aatattacct
    16861 ctacccattg cctcaataac tataccttta tagccactgt caacagcaaa tcttataaag
    16921 tcagcatttt ctcctacata tgctttaaat aaagcaacct tagattctac cttatcagta
    16981 tctataattg ttctatttac aatatctctt gtaaggacaa gttcattgca atcaattatt
    17041 cccaatggac cactagttag agatttaaat gtatttagag aaagagtatt tgttttagta
    17101 gcttcagaag ctaatagtac ttcattgttt aaaactacaa ggactccttt tcccatagca
    17161 tctttggata ctgcagtaca aactgatgca gataaattac ttgaaccatc atatccaagt
    17221 tcagaactac ttctcatagc acctgtgact attacaggtt tgatgttatc aattgtaagg
    17281 tctagaaaat aagctgtttc ctctaaacta tctgtaccat gcgttattac aacacctgta
    17341 atatcttttc ttgctaataa atcatttaca taatttttca attccatcat tctataaggt
    17401 gttatatgag gtcctggaat ttcatcaaag ttaaaaactt caacgtctgc aactttatca
    17461 atgttggtaa ccattgataa tatttgttcc cctgataaag ttggtatagc agctccgact
    17521 ttatcgtcta ctgtcataga tatagttcca ccagtaaaaa ctatggcaac ttttttctta
    17581 atactcatac tattctctgc ctttcataaa taaaatttat aattccgata ggattatatc
    17641 aaaaaaagta gttaaggtct acataaattc ttttattcat ataatttaga tatttcagtg
    17701 taatataatt atttcttaat tttttgccat tcaacaatat aaccatttag tatttctctt
    17761 atggcttttc ctatcttagt ataagcttca tcatatagat ttgctagaga tattctaatt
    17821 gaccattcag gaccatagaa tccatttcca ctaagtaaaa ctactgaata gtcattagct
    17881 aatttataaa gtacatctac tggagatgca tttttactta taaatttaaa taagtcttct
    17941 ccatagttta atttagccca ttcttcaata tcaaattgag tgtaatatgc agcattattt
    18001 ttattttcat taattggaag ctctaaagct tcaaataata gtttttgacg tctatggcag
    18061 atactcatat taagtttctt gtaggcatct actttatcaa tcaatgcaaa agcagagaaa
    18121 aatgccattt gtacttgttg tggagtagaa agaccagcag tatggttaag agcaacaagt
    18181 ctactatcag caacaactct ttccataaat gaaagactag aagggttaag actcatatca
    18241 ctgtaacgtt tatctactga tttttttagt tccccagtta aatcatttat tttcttatca
    18301 aaaacatttt tcttgtgaag agcaaacgta ccaagcctcc atcctgtaac tccaaagtat
    18361 tttgaataag aataaactcc aacagtgttg taaggtagct ttgacatcaa cgacgaaaaa
    18421 tcctctacaa aagttccata tacatcatct gatattatca tcaaatcttt attataattt
    18481 tcaattacat ctattagatt attacaagat gtttcatcca tagctataga agcaggatta
    18541 ttaggattaa caacgaataa agctttaatg gacttgtcac gaagtttctc taattctttt
    18601 tttgtatact gccatgtgtg agcacctttt tcgtcaactt cattagcatt tatatttaca
    18661 actttaaagt cataacgagg aaggttaggt atttcaagat aaggtgtaaa tattggagtc
    18721 attaaggcta tggtatctcc tttttttaga agttcatttg ccattaatga atcaaaaata
    18781 tagcacatag cagctgtagc accttcaaca gcaaaaattt ctacatcacc atatttacct
    18841 gatatatcat atttcatttc ttgaactagg taatcgtgta ctattttttc catatgaggt
    18901 aacattctat ctggaagtgg atagttatca ccaataattc catcagctag ttcatgaata
    18961 aactcatctc catcaaatcc aagttcattt ataccaaagt ttataatatc tctagctaaa
    19021 tatgctccag gcatatctat attttcattt ataaatttaa aaaatctttc tttaataccc
    19081 tttttttgag gcattccagc taaatgacct aaatcccaat tagaacgagt ttcattaatg
    19141 gcgaaatgac caaaagtaaa aaaggcttct ctggcagttg aacaagtcca gtttggattt
    19201 ccacgacctg catcaagtaa ttccctgtta ttttcctttg cagtatattt tgcaatatct
    19261 attaatttgt ccttaaattc aaaagggctt atttttccat atatgttttt gatttcttta
    19321 taagtatgat ttttcatgtt aaattcctcc ttatgtaata agtttcacat ttttataata
    19381 aaataaacaa gttacaaaaa tgattatact
//
LOCUS       DAMJVM010000065        19363 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.068, whole genome shotgun sequence.
ACCESSION   DAMJVM010000065 DAMJVM010000000
VERSION     DAMJVM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.068"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            130..498
                     /locus_tag="PC523_002459"
     CDS             130..498
                     /locus_tag="PC523_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2475971.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIRKCCKRREK"
     gene            501..872
                     /locus_tag="PC523_002460"
     CDS             501..872
                     /locus_tag="PC523_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HDF2475972.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTTVDAVIVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(1016..1471)
                     /locus_tag="PC523_002461"
     CDS             complement(1016..1471)
                     /locus_tag="PC523_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475973.1"
                     /translation="MNINTVQGDSIEVLLRQLGATKISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(1510..2775)
                     /locus_tag="PC523_002462"
     CDS             complement(1510..2775)
                     /locus_tag="PC523_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HDF2475974.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNEKAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
     gene            3244..4695
                     /locus_tag="PC523_002463"
     CDS             3244..4695
                     /locus_tag="PC523_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDF2475975.1"
                     /translation="MDFFNGILTSINNAVVFVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFSNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLENVSEWHRELPEEHLG"
     gene            complement(4905..5333)
                     /locus_tag="PC523_002464"
     CDS             complement(4905..5333)
                     /locus_tag="PC523_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2475976.1"
                     /translation="MKTYSIQDLTLDKRNLIKKYSLTLNEDLIWEFKHSKYHTIKYFT
                     HKFAVKESVLALLFNIHKLCYAKIKYFESNFSKYEPYKYSYQHGFVKCEMYDMNFILH
                     KYSNIMIDLRNLQEIKTIEEFRLFCDYLESFEDNLQDLII"
     gene            complement(5735..6196)
                     /locus_tag="PC523_002465"
     CDS             complement(5735..6196)
                     /locus_tag="PC523_002465"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDF2475977.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVST
                     KNIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDNELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
     gene            complement(6302..7495)
                     /locus_tag="PC523_002466"
     CDS             complement(6302..7495)
                     /locus_tag="PC523_002466"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HDF2475978.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDEGRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINGENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRVPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(7518..8183)
                     /locus_tag="PC523_002467"
     CDS             complement(7518..8183)
                     /locus_tag="PC523_002467"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDF2475979.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTTLLAKNVGDFVNLESDVIGKYVNNFMVNNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(8217..9329)
                     /gene="ribD"
                     /locus_tag="PC523_002468"
     CDS             complement(8217..9329)
                     /gene="ribD"
                     /locus_tag="PC523_002468"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDF2475980.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDDYI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLRDNNIDVTVGTLEEEARDLNEVFFYYIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESKKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNRLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLAINNVTLIDGDILIESDVLN"
     regulatory      complement(9461..9582)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            9771..12254
                     /gene="recQ"
                     /locus_tag="PC523_002469"
     CDS             9771..12254
                     /gene="recQ"
                     /locus_tag="PC523_002469"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDF2475981.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILNEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKAMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFLELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKSLPKRPIVTAFTATASEEVRIDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILNYFGENYMDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFINTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNVLRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFETVIKEYVEKNNINKKINEKNVDLKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPEYMILANKTLKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILEEVNKYVKENDINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHRISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      12343..12454
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            12596..13897
                     /gene="thiC"
                     /locus_tag="PC523_002470"
     CDS             12596..13897
                     /gene="thiC"
                     /locus_tag="PC523_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDF2475982.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKVKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            13951..14145
                     /gene="thiS"
                     /locus_tag="PC523_002471"
     CDS             13951..14145
                     /gene="thiS"
                     /locus_tag="PC523_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDF2475983.1"
                     /translation="MKVNGKEIEFEENLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            14138..14941
                     /gene="thiF"
                     /locus_tag="PC523_002472"
     CDS             14138..14941
                     /gene="thiF"
                     /locus_tag="PC523_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HDF2475984.1"
                     /translation="MVKIKIFVNESLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMPLCENDKLTLIQKGIKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DVFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            15004..15774
                     /locus_tag="PC523_002473"
     CDS             15004..15774
                     /locus_tag="PC523_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDF2475985.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPIQMA
                     RAFKLAVEGGREAYIAKTGSVSEFANASSPLTGFLGNL"
     gene            15791..16894
                     /gene="thiH"
                     /locus_tag="PC523_002474"
     CDS             15791..16894
                     /gene="thiH"
                     /locus_tag="PC523_002474"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDF2475986.1"
                     /translation="MSFYDVIEKYRNFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEAMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPYIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            16895..17584
                     /locus_tag="PC523_002475"
     CDS             16895..17584
                     /locus_tag="PC523_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDF2475987.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELKN
                     LYMKIKTKINCIDFQEQISDESLKTNINQKGCGNKFKVNFIINSNIEFFEKMDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVDEVEYLEKLIRKQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAVMSTIMKSKDIK
                     KTISNYNEKLN"
     gene            17770..19164
                     /gene="yfcC"
                     /locus_tag="PC523_002476"
     CDS             17770..19164
                     /gene="yfcC"
                     /locus_tag="PC523_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDF2475988.1"
                     /translation="MTDSKKKRFVIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLAGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
BASE COUNT         7203 a         2357 c         3117 g         6686 t
ORIGIN      
        1 ttaaaagtta tattagaaag aattagaatt atttataata tataccaaaa tttaaaaata
       61 atggttgaaa aatctaatta agagtactat aatttaatta agtaattact taatcaaaac
      121 gaggtactta tggaaaactt ggttaaaata tttaaagcat tgagtgatga aactagactt
      181 aacatactta tattagtatc caagagaaat atatgtcaaa aggggatttc taaatatcta
      241 gggatttcag attctgctgt ttcacaacat ataaaaatat taaaagatgt tgggataata
      301 acaggatata aagaaggata ctatgtttta tatcatatta ataaagaatc atttaatata
      361 tgtgtagatt ttataaattc tatgttaagt aattctagtg aaagctttat agatgtattt
      421 gatgtaaata caatacatct aggatgcagt aaagaatgta aatctataag aaaatgttgt
      481 aagagaaggg agaaataata atgaaagtat gtataccagt agaagagaat aagggattgg
      541 atagcaaacc atatgggcat tttggttctg ctccaatttt tgttgtgtgt gatttagaaa
      601 gtggagaagt taagtcttta gataatggcg atttagatca tgaacatgga aaatgccaac
      661 cattaaaagc attgtcaggt acaactgtag atgcagtaat agttggagga ataggacaag
      721 gagctataat taaattaaat ggaatgggaa taaaagttta cagagccgaa ggcgatacta
      781 taagtgctaa tttagaccta cttaaaaatg gaaaattagt agagtttcca tcagaccata
      841 cttgttcaca tgatggatgt ggacatcatt aaaaaaaata gctgatgaaa catatgcttc
      901 atcagctatt ttttctttta ataaatacga ttttattctt ttaaaaccaa aatttagatt
      961 tttatgtttt ttgtaatatc ctcgataaca tctcgagttt ataagtttac tacttttaga
     1021 tttttttcgt atgtttttct atatagtcaa tatcatttaa tatgtcatta agctctttat
     1081 taagtgtttt taaatcatca ccatctacat tgtagtttaa aaatagttct tctacattat
     1141 caatgataga attaactttt ctagtaactt caacaaatgt tttaaagtta ctactatttt
     1201 tagcatttag aaattttttt aaatcctttg atataaatga tactatttca tattcgtcat
     1261 taaatagacc tctaccaata ggatatggtt caattctctg taaaaaatat tggtctttag
     1321 cattaatatt ataagtataa gatatttccc aaccgtctcc taaatcaaac ttaataaaat
     1381 ataatgtact acttactttt gatatttttg tagcaccaag ttgtcttaaa agaacttcaa
     1441 tcgaatcacc ttgaacagta ttaatattca tatatacaca tcccctttag tattattata
     1501 ctatattttt taaaatttta taattaaata aacagtagat ataataatag atataatcat
     1561 aagtggaaaa cctattttga aatactcttt aaaagagata ggatgcccgt gtttattacc
     1621 tatacctgct agtacaacat tagcagatgc tcctattagt gttccatttc ctccaagaca
     1681 agctccaaga gatgttgccc accatagtgg cattacatca ataccttcag cttgcatagt
     1741 taaaataagt ggaattagtg ttgcaacaaa tggaatatta tctaagaaag aagagactat
     1801 cgctgataac catagtatca acaacattgt aaatacaaga tgtccttctg taagtccaat
     1861 caatgcctga gccaatttat ttattatacc aacttctact aatccaccaa ctactacaaa
     1921 taatcccata aagaataaaa ttgtagacca ttcaatacct gccataattt catctacatc
     1981 ctgttttcct attagaagca ttatagatgc tcctgtcagt gctacaacag aagagtctat
     2041 atggattgta ctgtgaaaca taaaaccaaa aagtatgaat gctattaata taagactttt
     2101 tataagtaga ggtttatctt taacagattt tttttcatcg agtttcaata ttgcgttttt
     2161 agctttttca tttacaacaa gttcttttcc ataaataaat ctaaagcata ttatagttat
     2221 agcaagtatt actataacag caggacctaa atttattaca aaatccataa aactaagatt
     2281 ggcagcacta ccaatcataa tatttggagg gtctcctata agagtagctg ttcctcctat
     2341 atttgaagct aaaatctgtg ttattaaaaa tggaacagga ttaagtccta atatttgtgt
     2401 aataactata gtcattggtc caataagtaa gacagttgta acattatcta atacagctga
     2461 taaaatagct gttataatag caaaacaaat cattatcttc catgggtcac ctttagcttt
     2521 ctttgcagtc cagatagcta tgtattcaaa taatcctgag tttttgacaa cagctacaaa
     2581 tagcatcata ccaactaata caccaatagt gttgaaatct atatggctta atccttcatc
     2641 aagcgtaagt atattgaata ttatcattaa tagtgcacca ccaacagctg ctacggatct
     2701 gtttaccttt tctgtaataa tagctgccat aactattaaa aatatacata tagctattat
     2761 ttgttgaaat tccataatat aagtacctcc atttgtatta ttttgagcct aatgctagta
     2821 ctatatatag tattactttt ttattgtttt ttcaatggat gtatgaaagt atattattta
     2881 acaaagtttt tgaaaaattg acagaattta aagaaaattt ttgttattta aataaatttt
     2941 aaaatcattc cattgataat aatattaaac gacaaacgaa aaaatatacc cttcaaacta
     3001 gaatttattg taacaaataa caattataaa atattaaata tatctatatt ataaaaacag
     3061 taaaaaaagg tttaatctga atataaaact gcaatatatt aaaaaaatta ttcattttaa
     3121 gccttgtaat ttgattttat aaatattata attaaattaa tgaattttta acaaggaatt
     3181 attcattttt ttgattttaa gatttattga gaaaacaaaa ctaaataggg ggtaattaat
     3241 ctaatggatt ttttcaatgg tattttaact agtatcaata atgcagtagt atttgtaaac
     3301 aattacttat ggtcgtatat cctaattata atgttagttg taattggtat ttactttact
     3361 atcaaaacaa attttgtaca attcagatat tttatagaaa tgtttagact tctgggtgat
     3421 ggtacagcaa ataaaaatgc aaaaaaggaa ggaaaaatat cttcattcca agcattttgt
     3481 ataagtacat cttcaagagt tggaactgga aatatagctg gaatagcaat tgcagttgta
     3541 gcaggaggtc ctggtgctgt attttggatg tggcttatag cattgattgg ttctgcatca
     3601 agctttgttg agagtacatt agctcaaatt tataaagtta aagatggtca agcttttaga
     3661 ggtggaccag cgtattatat ggaacaaggt ctaaataaaa aatggatggg tattttattc
     3721 tcaatactta taactatatg ttacggtttt gtatttaatg cagttcaagc aaatactgtt
     3781 tcacttgcat ttagtaatgc atttggaata agtaaaatgg ccatgggaat aggtcttgga
     3841 attttaactg caatagttat ttttggtgga gtgcatagag ttgcaaaagt ctctgaaata
     3901 atagttcctg tatttgcagg attatatata ttagttgctt tagctatagt tgtaatgaat
     3961 ataacagaga taccaagtgt tatagcgttg atagtggaaa gtgcatttga ttttaaagga
     4021 atggcaattg gtacatttat gggagttgta atgactggag tgaagagagg tctattttct
     4081 aatgaagcag gtatgggtag tgctcctaat gctgctgcaa cagcacatgt aactcatcct
     4141 gtaaaacaag gtctaattca atctttgggt gtttttacag atactataat aatatgtagt
     4201 tgtacagcat tcatggtatt attatattca ggttatagta gtagtggagc aactggaatt
     4261 gaattagctc aagaagcttt aacacatcat ataggaccag ttggaaatat atttatagca
     4321 gtttgtatat tcttgtttgc gtttagctct attgttggta attactatta tggtgaatca
     4381 aacatggagt ttatgagtgg aagtaagact aaacttaatg tatttagagt ttttgttgta
     4441 ggtatggttt tatttggttc acttactcaa gtagatgtag tttggaacct tgcagactta
     4501 tttatggcat tgatggcgat aataaactta atagcaatag cacttttagg aaaatatgca
     4561 tttattgctt taaaagatta tacaagtcaa aaaaaatcag gaataaaaga ccctgtattt
     4621 attgcagatg aaatagaagg tctagaaaat gtatcagaat ggcatagaga attacctgag
     4681 gaacatttag gataaaaaaa tagtataaaa ttatgaaatc attttgaaag attagagtat
     4741 ggatatactt tacatgaata tagtattgaa ttaatttata ttactggaac ttttaattcc
     4801 agtaatattt ttttattatg taatttaact ttattaaaag ttttttatca ttgaagatag
     4861 attgtatatt ataaatttta tctttgggtt tcttcttaaa aatattatat aattaaatcc
     4921 tgtaaattat cttcaaagct ttcgaggtaa tcacaaaata atctaaactc ttctatagtt
     4981 ttaatttctt gtaaatttct caaatctatc attatattac tatatttgtg taagataaaa
     5041 ttcatatcat acatttcaca tttgacaaat ccatgttgat aactatattt ataaggttca
     5101 tattttgaaa aattcgactc aaaatatttt attttagcat agcacaattt atgtatatta
     5161 aacaagagtg caagaacaga ttccttaact gcaaatttgt gtgtgaagta ttttatagtg
     5221 tgatatttac tatgtttaaa ctcccaaatt aaatcctcat ttaaagtaag agaatatttt
     5281 tttataagat tacgcttatc tagtgttaaa tcttgtattg aatatgtttt catagtttat
     5341 tctcctcata aatatctaat aagtattaag caatagtatt ttattattaa ttactcttaa
     5401 ttatatttta tggtaaatta aaaaagtaat aaaatagata attagtatac tattagtctg
     5461 ttctataaat aaaaacaata gcttaaatac taatatgctt acttttatgg tattttatgt
     5521 ttggaattat atttgtaatt actgtaagat tttcaaagta gatttgatgg ataatgtata
     5581 attttagaaa gggatgtaaa ttgttttgtg agatttatat acatgattat attaaaaata
     5641 ctaagtatca aaagaagaaa tatagcttct ctctttggta cttagtattt tatatctttt
     5701 acactaattt ttaatattta attaataata ttatttaatt tagagactta aataaatttg
     5761 ccatttctaa tgcattcata gcacaatcat aacctttatt tccagctttt gtaccagctc
     5821 tttcaatagc ttgttcaata ttttctgtag ttacaattcc aaatataact ggtaattcat
     5881 tgtctaaaga tacttttgct atccctttag agacttcact acatacataa tcataatgag
     5941 aagtagctcc tcttataaca catcctaaac agattatagc atcatactta ccacttttag
     6001 ccattttttg agcaacaaga ggaatctcaa aagctccagg tacccaaact atttctatgt
     6061 tttttgtaga aacatcgtgt cttaaaaggc aatcttcagc tcctgataaa agttttgatg
     6121 ttataaattc attaaaacga gaatttatta tacctatttt taaatctttt ccaataagtt
     6181 ttccttcata tatcattttt atttcctcca attttattta tgttatagat ttaaatttat
     6241 aaatatcttt aagtttaatt ataaaaaatt acttagtaga aaaatatccg catataatcg
     6301 cttatataat atctaaaaga tgtcccattt tatctttctt agtttttaga tatagcttat
     6361 taaagtcatt aatttcttca ttaataggga ctctattttc gatttttatt ccatatttct
     6421 ctaattgatt tattttatca gggttattac ttaaaagatt tatacttttt atgtttaaat
     6481 tttttaatat ttgagcagcc atatagaaat ctctcatatc ttcttcaaat ccaagcatta
     6541 aatttgcttc aacagtatca taaccttctt cttgaagttt ataagcttta attttattta
     6601 aaagaccaat acctctacct tcttgcttca tataaattaa aactccactt ccattttcta
     6661 caattttctt catagaagat tctaattgta atccacaatc acattttaaa gagtgaaaca
     6721 catctcctgt taaacattca gaatgaagtc taactagagt attttctcca tttatatttc
     6781 cataagtaag agcaatatgt tcttcatttg aataagtgtc tctatatcct atgatttcaa
     6841 agtttccata ttttgttgga aggaaagcac tagatacttt ctctatagta gtttcattta
     6901 ttttgcggta ttctataagg tcttttatag tgatgatttt taaattatgc ttttctttga
     6961 aaatcatcaa atcatcaact cttgccattg taccatcatc tttcattatt tcacatataa
     7021 gaccaatctc tttaaaacct gcaagtctag caaggtctac tgaagcttct gtatgtccat
     7081 ccctaacaag aactccacca gtttttgcga ttaaaggaaa gatgtgacca ggagttttaa
     7141 aatctttact tgtagaattt tcgtcacaga acaattttac agtttcagct ctttcatatg
     7201 cagatatacc agtcagagta tctgccccat ctatagtaac agtaaaggct gtttggaatg
     7261 tatctgtatt ttctctaacc ataagaggta tattaaggct tttaaatttt ttttcagtag
     7321 caggtagaca aattagacct ctaccatatg tagccataaa atttattgat tcaggagtag
     7381 ccatttcagc tgccatcaat aaatctcctt cattctctct accttcatca tcaactacga
     7441 ttaccatttt cccatttttt atatcttcaa tagcttcttc aatagtatta aacatgtaat
     7501 tcactccttt attattttta aaaaccattt ttaaataaaa aatctttatc tatatttgat
     7561 ttatggttac tactattaga atttaattcc ttgtagttat taaccataaa attattaaca
     7621 tacttcccaa ttacatcact ttctaaatta acgaaatctc caacattctt tgccaagaga
     7681 gtagtgttaa ttttagtatg aggtataata gaaaccttaa atatttcatc atctaaatat
     7741 gatatagtta aacttacacc atcaatagca acagaacctt taagtatcat tgatgataaa
     7801 agttttttat caggtcgtat actcactaaa actgcatttt catctttttc aaatttacag
     7861 atagttcctt taccatcaac atgacctgtt actatatgac caccaaatct cgaagaaaga
     7921 ctcatagctc tttcaagatt taccttgtca tttgcattta ataaacctaa atttgtggaa
     7981 cgaattgtct ccatcataac atcagctgta aattcatttt ttccgagatt tgaaacagtt
     8041 agacaaactc catttacagc tatactatct ccaagatttg tgccatctaa tattttattt
     8101 gatagtatgg ttacttttcc actcttacca ttagcagtaa tttttctaag tattccaact
     8161 tcttctacta tgccagtaaa catcctgacc tcctatatac atgtattaat aaaaatttaa
     8221 tttaaaacat ctgattctat aagaatatct ccatctatca aagttacatt attgattgct
     8281 agctgtgttg aatctgacaa atgatttatt ccttctccag aaacgaatgt aggtgcatct
     8341 ttaccaccta taatcttggg ggatagaaat aatttaacct tatcaactag tctatttcta
     8401 aagagactgt cattaagtgt agcaccacct tcaactagaa cagaatcaat gtttaattct
     8461 cctaatttat acattacaaa ttccaaatct actaagttat tttttgaagg agaaattatt
     8521 gtttcaacat tattttcttt taacatagat aattttttac tttctttgca acatgtgaaa
     8581 actatagttt tactggtttt atctttaacc aaattactat taagtggcaa ctttaggtgt
     8641 gtatctatta ctattcttgt aggatgagat actttttctt gattaagtct acaagttagt
     8701 aagggattat cgttcaaaac tgtatttatg ccaaccatta tactttggta tttatttctg
     8761 tatttatgag ttaggtatct tgatgattca ttagatatcc actttgattc tagtgatttt
     8821 gtagcaattt taccatctag acttactgct gattttacaa tgcaaagagg tcttttgttt
     8881 tttatataat aaaaaaatac ttcattcaaa tctcttgctt cttcttctaa tgttcctaca
     8941 gtaacatcaa tattattatc tcttaatttt tttactccat taccacaaac aagtggattt
     9001 gggtctaatg tagatatgac aactctttta atcttatttt gaataatttt atcaacacaa
     9061 ggaggcgttt ttccataatg agaacatggt tctaaattta cataaagagt agagtctttt
     9121 agactttgtt tagcactgtt tatagcattt acttcagcat gattagagcc aaatttctca
     9181 tgatatcctt tacctattat gtaatcatct ttaacaatta cacatccaac taatggattt
     9241 ggatttacaa agccttctcc atttttagcc aattcaatag cttttttcat ataataaata
     9301 tctttttctt tttgattcac aaaattcaca atatgtacac ctcctgttta attggataat
     9361 ttttaaacaa aaaaccccga gaattaactc agggtttaga ataatctaga ataaatgttt
     9421 aataaatgta caacaaaatt gctctacaaa aacactcatt aaatatcttc ttccatccag
     9481 actttactgt cggctttgga atttaaccaa atcaaccaag ttttacattg gctcgcgggc
     9541 tataccgccg attgggaatt tcaccctacc ccgaagatta agtattaaat ttatatttag
     9601 aatgtatcat aaattatttt actttgcaaa ctattttttt taaaaatata tgttaatata
     9661 taatatagaa tgtctataaa ctttgaaata ccacgattgt gaatataaaa ataaaaattt
     9721 gaacataatc aggaaagaag tctaattaat ttaaattagg agagtaatat atgaataaaa
     9781 aacttttaga aatactatca aaatattatg gatatacaag ttttagaaaa ggtcaggaat
     9841 ctataataaa ttctatctta aacgaaaaag atgtattagc tattatgcca acaggagggg
     9901 gaaaatcaat atgttatcag attccagctt tgattttaga tggaatgaca atagtaattt
     9961 ctccacttat atctcttatg aaagaccaag tagatgcatt aaaagctatg ggaattagtg
    10021 ctgcttttat aaatagttca ttaagtagca aagaatttaa tgaaatatta aacaatatta
    10081 gaaaaaataa ttataaaata ctctatattg ctccagagag attagatgca caggagtttc
    10141 ttgagctaat aaataataac aatgtaagtc aagttgcaat agacgaagca cattgtgtat
    10201 ctcaatgggg acatgatttt aggttgagtt atagaaggat ttctgatttt ataaaaagtt
    10261 tgcctaaaag acctatagtt acagcattta ctgcaacagc ttcagaagag gtaagaattg
    10321 atataataaa tttactgtgt ttagaaaacc cagattatta tataactgga tttgatagag
    10381 agaatttatc gataaatata gtaaaatcat ctagtaaaaa taagtatatt ttagattata
    10441 ttcaaaacca taaaaatgag tctggaatta tatatgttgc aactaggaag gaagttgaaa
    10501 atatatataa tggtttatct aagagaaata tttctgtgtc aaaatatcat gcaggtctat
    10561 ctaaaaacga aagaagtaaa aatcaagaag actttataaa tgataatgtg gatataatgg
    10621 tagcaacaaa tgcatttgga atgggtattg ataaacctaa tataagatgg gttatacatt
    10681 acaatatgcc tcaaagtata gaaaattact atcaagaaat aggtagagct ggtagggatg
    10741 gagaaaaaag tgagtgtgta cttttatttt ctccaggaga tgtacatata caaaaatatt
    10801 taattgatat tggagttgaa aatcctgaaa gaaaattatt tcagcataag aagcttcaat
    10861 atatgattga cttagtttac agtaattcat gctatagaaa gacaatattg aattattttg
    10921 gagaaaatta tatggacaat tgcaataatt gtagtaattg tttagtagag ggtgaaatag
    10981 tagataaaac tatagatgca caaaaagtca tttcatgtgt agctagaatg aaaaggagtt
    11041 atggtgttac tactattgta gatgtactta gaggttctaa aaataaaaag attttacagc
    11101 ttggactaaa tactttgtct acatataata ttatgagaga ttattctagt gaagatttaa
    11161 agaattttat aaatacatta gtatctcatg gttttttaga tttagttgag actctaggta
    11221 atggaaatag aggtagtttt ccaactataa gattaaatga aatgtctatg aaagtgttaa
    11281 aaggtgaagt gaaagtagaa tttaaagaga ttgtgatgac aaaatcattg gaagtagaag
    11341 atgagttata taatgtttta agagaactta gacattcaat tgcaagtgaa gaaaggatag
    11401 ctccatatat ggtatttgga gatggcacgc ttagagccat gtctagtagt tatccaatta
    11461 ataaagaaga aatgctagat atctctggag taggagaaat taaatatcaa aaatatggta
    11521 gagattttga aacagtaatt aaagaatatg tcgaaaaaaa taatataaat aaaaaaataa
    11581 atgaaaaaaa tgtcgattta aaaaacgtta atagtgattt cctagaggtt aatacagata
    11641 aagttctata tgaaaaactg ttagtcatta gagaggaata tgcaacgaag gaaaaattac
    11701 ctgaatatat gattttagct aataagactt tgaaagaaat aagtggaagg tatccacttg
    11761 atgaagaaca attaaaagat atttctggaa taggtggggt aaagtttgaa aagtatggag
    11821 ctagtatttt agaagaggtt aataaatatg taaaagaaaa tgatattaat gttatttggg
    11881 agaaaaaggg gaaaaggaag ttaatattag atggagaaag tagaaaaaat aatgaaatag
    11941 ccttagattt actgaatcaa ggaaaagaca tacaaagtat aagtcaagaa atagaagttt
    12001 cactgtcaac cattattgga tatgtgcatg actatgtaaa ggatggacat agaatatcat
    12061 ttgaattagg gttagaaaat ttttataatg aagataaaaa gaagatgatt ttagaagttt
    12121 gcgaaaaagt aggttatgat aatttaaata acataaaaag aaaacttcca gattcaataa
    12181 agtatgaaaa tataagagct gtgatacttg actcttatct tgaaaactta aaaaataata
    12241 ttatagcaga gtaattttta acttaataaa aaataaaatt tgtattatta tgttgaacta
    12301 atatttttgc tgtgttatac tgaatttgta aaaataatga aaaattagag ctaggggcgc
    12361 tagaattggc tagctgagag ataaagccgt aacttacaaa ccctaataac ctgatcaaga
    12421 taatgcttgc gaagggaagc tttttaatat aattttatta tataaatagg caaattattt
    12481 aatgttaggg taaatttgtc taagtgtata tacgattata agagtgcttt ccaaacaagg
    12541 aaaagcactt tttttatttt caaaaactat aactttaatc ttggaggatt gtaatatgaa
    12601 ctatacaaca caaatggatg ctgctagaaa aggcataata actaaagaaa tggaaatcgt
    12661 ttctcaaaaa gaacaggtag atgtaaatga attaagagaa ttaatagcaa atggacaagt
    12721 tgttatacca gccaataaaa atcataaatc tttaagtgca gaaggtgtag gtaaaaattt
    12781 aagaacaaaa ataaatgtaa atctaggaat ttcaagagac tgtaaggata tagaaaaaga
    12841 acttgaaaag gttagagtag caatagacat gaaagctgaa gctataatgg atttaagtaa
    12901 ttatggtaaa actagagaat ttagagaaaa agttgttgaa atgtcacctg caatgatagg
    12961 ttcagtacct atgtatgatg cagtaggata tttggaaaaa gaattaaaag atataacaga
    13021 agaagagttt ttaaatgtaa taagacaaca tgctattgat ggagtagact ttattactat
    13081 acatgcaggt cttacaagaa gtgtttgcca aaaagtaaaa aatcatgagc gattaactca
    13141 tatagtatct cgtggaggtt ctttattgtt tgcatggatg gaattaaata ataaggaaaa
    13201 tcctatttat actaattttg ataaaatatt ggatatatgt gaggaatatg atgttacatt
    13261 gagtttagga gatgcttgta gaccaggttg tataaaagat tctactgacg gtgttcaaat
    13321 tcaagaactt gtagtattag gtgaacttac taaaagagcc tgggaaagaa atgtacaagt
    13381 aatgatagaa ggtcctggac acatggctat tgatgaaata gaagcaaatg tagtattaga
    13441 aaagagatta tgccatggag caccttttta tgttcttgga ccattagtta cagatatagc
    13501 accaggttat gaccatataa caagtgcaat aggaggagct ttagcatgtg ctaaaggagt
    13561 ggatttttta tgttatgtaa ctccagcaga gcatttaaga ctgcctaatt tagatgatat
    13621 gaaagaaggt ataatagctg caaagatagc tgctcatgca ggagatatag ctaaaaatgt
    13681 taaaggtgct cgtgaatggg ataataagat gagtaaagct agagctgatt tagactggtg
    13741 cgagatgttt aggcttgcaa tagaccctga aaaggctaag agatacagag atgaatcaac
    13801 tcctactcat gaagatagtt gtactatgtg tggaaaaatg tgctcaatga gaacggttaa
    13861 gaaaatatta aacaatgagg aacttaattt aatataattg ttggatagta tgactaaagt
    13921 aaaaagaagt tgaaaagagg aggtgattta gtgaaagtta atggtaagga aattgaattt
    13981 gaagaaaatt tgactgttat agatttatta aataaatata atttaaagag tgatagagtt
    14041 gtagttgaag tgaatttaga gataattgaa gaatctaact acaatacata tgtattaaaa
    14101 gacgaggata ttgtagagct tattagcttt attggaggtg gttaaaataa aaatatttgt
    14161 aaatgaatca ttaacagatg ttgaggaaaa tacaactgct tataaagtta gagatagcta
    14221 taacaataaa tgtgatgtat tagtgttaaa tggatatcca attaaaacag atatgcccct
    14281 ttgtgaaaat gataaattaa cacttataca aaaaggcatt aagccatcac ttgatgagtt
    14341 ggaaagtttg ctgattgcta gacatactcc aaatatacat aataagttaa aaaaagggaa
    14401 ggttgctata ttgggattgg gaggacttgg ttccaatata gctatctctt tagctagaat
    14461 tggagtggga gaacttttac ttatagatta tgatgttgta gaaccatcta acttgaatag
    14521 acaacagtat tttatagatg atataggaaa actaaaaact gatgccatga tagaaaacat
    14581 aaaaaaaata aatcctttta taaaattaaa taaacgagat gtttttttaa acaaaaataa
    14641 tatggacact attaaagact cagatttaat aattgaggca tttgatgatg cttcatgtaa
    14701 ggcacaggta tgtaattatg tattaataaa tttaaaagat aaatatctaa tagcatcatc
    14761 tggtatggca ggttattatg attcaaatat aataactact aagaagataa aagacaggtt
    14821 ttatatatgt ggggactttg tgaatgaagc taaatttggt gaaggactta tggctccaag
    14881 ggttgctatc tgtgcaaatc atatggcaaa tttggcaaca caaatactta tagatatgta
    14941 aatattaaaa ttagacacta aaaagattga atattaaaaa aatttaatat ggagggaaat
    15001 aatatggata aattggtact tggtggacat gaatttaata gcagattatt ggttggaaca
    15061 ggtaaatatg gttcaaataa tatattgcct gaggtaataa aagaaagtgg aagtgaaata
    15121 ataacaatgg cacttagaag agtggattta gacaataagc aagaaaacat attaacatat
    15181 attccaaaag aaatgacaat acttccaaat acatcaggag ctactaatgc agaagaagct
    15241 gtaagaatag ctagaatatc tagaaaaatg ggatgtggag attttataaa aatagaagta
    15301 atatcagata ctagatatct acttcctgat aatgaagaaa ctataaaggc tacaaagata
    15361 cttgctgatg aggggtttat agttcttccg tatatgacac cagaccttta tgctggaaga
    15421 aggcttatag aagctaatgc agcagcagtt atgccacttg gagctccaat tggttcaaat
    15481 agaggtcttc aaatgaaaga gatgataaga ataatgatag atgagttgga tataccaata
    15541 atagtagatg caggtattgg aaaaccatct caagctatgg aggctatgga aatgggagct
    15601 gatgcagtac ttgtcaatac tgctatagct tcagcaggag accctataca aatggctaga
    15661 gcatttaaat tagctgtaga aggtggaaga gaagcatata tcgcaaaaac aggaagtgtg
    15721 tcagaatttg caaatgcttc ttctccttta acaggttttt taggcaattt atagaaagtg
    15781 gtgattggtg atgagttttt atgatgtaat agaaaaatat aggaattttg attttgatgg
    15841 atatttaaat aatgttactg ataatgatgt tttaagaagt ttatcaaaag acaagcttga
    15901 ggattttgat atattaaatt tgctttctaa aactgcagta aaacatttag aagccatggc
    15961 acaaaaagca cataaattaa gtgttcaata ctttggtaaa acagtatgtt tatatacgcc
    16021 aatgtatata gcaaattatt gtgttaatca atgtgtttat tgtagctata atataaaaag
    16081 tggaataaaa agaaaaaaat taaccatgga tgaaattaga gaagagggag aagcaatttc
    16141 aaaagaaggt tttaaacatc tacttgtctt gactggagaa agtagttttc actcaagtgt
    16201 agaatatata ggtgaggcta tagaaatatt aagagaaaaa tttccctcta tagggataga
    16261 agtttatcca atggaagtag aagaatataa gtacattgta gataaaggtg ttgaaggact
    16321 aacagtatat caagaaactt atgacgagga aatatataag agagtacata taaaagggcc
    16381 aaaatcaaat tataaattta gactagatgc tcctgaaaga ggggcaaaag caggtatgag
    16441 gactttatca ataggtgcac ttttaggatt aaatgatttt agaaaagaaa ctttttttac
    16501 aatattgcat gggaaatact taaaaacaaa atatccttat attgagcttt catattcaac
    16561 accaagaatg agacctttta aaggttgttt tgaggaattg gtagatataa gtgatactga
    16621 tttagttcaa gctatggtat gtatgagatt atttgaccct catgcagcta taaatatatc
    16681 aactagagaa aatttagaaa tgagaagtca tataatacct ttgggagtaa ctaaattgag
    16741 tgcaggtgta tctactgatg taggaggtca ttctcaagat gaacatgata cagctcaatt
    16801 taagataaat gatgaaagta ctgttaaaga tgtggagaaa atgctaaact caattggtta
    16861 tcagcatgtg tttaaagatt gggaaagatt ttaaatgtat ttgataacaa acagaaagtt
    16921 atgtagtgaa gaacgatatt tagaagttat taaagagtct atattgagtg gtgtagaaaa
    16981 tatcataata agagaaaagg atttagaata tcaagagcta aaaaatttat atatgaaaat
    17041 aaaaactaaa atcaattgta tagattttca agagcaaata tcagatgaaa gtttgaaaac
    17101 taatataaat caaaaagggt gtggaaataa attcaaggtt aattttataa taaatagtaa
    17161 cattgaattt tttgaaaaaa tggattgtca aggtatccat cttcctttta aattattctt
    17221 gaatttaatt gaaaataagt ataattttaa tgaaaataaa atactaggat tatctttaca
    17281 caaagtagat gaagtagaat atttagaaaa attaataaga aagcaaaata taaaaataga
    17341 ttacataaca ttatcacata tatatgaaac taaatgtaaa gaaggattaa atccaaaagg
    17401 aattgagctt ttaaaagaag ctaaaaagat tactgatatt aaaataattg cacttggggg
    17461 aattttacca tctaatgtca aagaaacatt aaaatactgt gatgattttg ctgtaatgtc
    17521 gactataatg aagtctaaag atattaaaaa aaccatatca aattataatg aaaagttaaa
    17581 ttagtaaata tagtatgttg aatttttaac ttaaataaaa ataagaaagc tatcttttaa
    17641 attttaaaag gtagcttttt attttgtatc tttgctaata aaaaattgta aaaagggaaa
    17701 actttagtca gagagattaa caatatccta gaaagattta aaatttagta aagtatggag
    17761 gacattataa tgactgacag taaaaagaaa cgatttgtta taccacacac ttttacaata
    17821 atatttttac taatagtctt aatggcaata ttgacttgga tagtaccaag tggtgaattt
    17881 agtagagaag atgtagaagg cagaatggta gttgtaccag ggacttatca aagtgtagaa
    17941 agcaatccac aaggatttgc agatgttttt aaagcaccaa tagaaggatt tatcgattca
    18001 gctgaagttg taggttttgt tttaatagtt ggaggtgctt ttggtattgt aaataggact
    18061 ggagctatag aagcaggaat agcttcggtt atctctaagt ttaagggaaa agaacttctt
    18121 ataattccaa tatcaatgat tttatttgga cttggaggta caacatttgg gatggcagaa
    18181 gagacattac cattttatat gatatttgtt ccacttatga catccttggg atatgattct
    18241 ctaacagcga tagcaattgt atttatagga gcagcagcag gagccgcagc ttcaacaata
    18301 aatccatttt cagttggtat tgcacaggca ctagctcaac ttgctcctgg ttcaggagta
    18361 acttatagag tagttatttt tatagcatat atactaataa gtatagcatt tgtcatgtac
    18421 tatgctaaga aggtaaagga aaatcctgaa agttcaatag tatatgaaat agataaaaat
    18481 aataaatcaa tacttaatca aacttcaaca gaaataaaag aatttacttt taaagaagta
    18541 tctgttcttg ctatatttgg aatagggatg gcaataatga tttggggtgt tttaagtcaa
    18601 gggtggtaca ttccagaaat atcaatgtgt tttgctgcga taggtgtgct ttctgggctg
    18661 gctggtagat tatctcaaat tgaaatttca gaaagtttta tagatggagc aaaagattta
    18721 gcatcagcag ctcttgccat agctttagct cgtggaatcg taataattgc tcaaaatgga
    18781 tttataatag atactatatt aaattccgca gcagcttttt taggtgggct tccaaaggtt
    18841 atatttgtat atttatcatt tttcttggaa gctgcattgg catttttaat tccatcatct
    18901 tcaggacttg cttcacttac agtacccgta cttgctcctc ttggtgattt ggttaatata
    18961 tcagcacaag tgatagtaac tgcttatcaa tttggtgtag gtcttacaaa ctttataact
    19021 ccaacttcag gagttttgat gggagcactt gcagtagccc atataccatt ttcaaaatgg
    19081 gtaagattca taatgcctat tctgttgatt ttaacagctg tatctctagt ctttttaaca
    19141 ataggcattt atatagggtt ttaattaagt atatttttat ttaagatatt tagtaatgat
    19201 ataaaatgat aatatgtttt caataaatta ccactaattt aattagtggt aatttgtttt
    19261 ttgtatttag aaataaatgt atttacttgt gttttgaatt attatctact tagacatagt
    19321 tttaattgtt atatttttat ttgattaagc attgatttgt tat
//
LOCUS       DAMJVM010000066        18583 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.069, whole genome shotgun sequence.
ACCESSION   DAMJVM010000066 DAMJVM010000000
VERSION     DAMJVM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18583
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.069"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            98..1699
                     /locus_tag="PC523_002477"
     CDS             98..1699
                     /locus_tag="PC523_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HDF2475989.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADRQTYETVILVNTEKSLADGLSASGLSGVTKAPILFTQKNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELNSKNIEVKRIGGEDRLKTSYLMAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKGIPFDVKNVQSYCIGSEEIMSNSLVKNTNSVR
                     IEGTDRFETNKNVIDYFFKNADGFYVSDGYQLVDAIAAAPLTKDSPMVLVNDGSDKTV
                     LEGAKNITSVGEIDEKVVQQCTNASKSNGQPPTITIGNIEIYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTEKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKETLLVSSKLEDMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKKVQTTQDAKDLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
     gene            1917..3506
                     /locus_tag="PC523_002478"
     CDS             1917..3506
                     /locus_tag="PC523_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2475990.1"
                     /translation="MKVNKRILSIGLTISLIMAGAPNINALSSIEKIQGKDRYETAAK
                     IAQKQTYENVVLVNTDNTLADGLSASGLAGAVKAPILLSQRNNIPPDTEKMLKDVKKA
                     YIIGTEDSIGKSVENELKQKGIEVKRIGGDDRIETSYLIAKEIASIKSINKVFITNGY
                     TGEADAMSASSVASRDGAPIILTDGKNVPFEKKEGVQCYALGSEEIISNDLVKKTNAV
                     RLAGEDRFETNKKVIKHFYSSAKEFYLSKGYQLVDAVAGSSIAKNAPIVLVDGNSDKS
                     VLRSADKITALGGIDEKTLEQCLSASSLDASAPTITVGNLNIYQGDKFDISKLNILAK
                     DSNGNDLTPELIGNINTDKVGKYKVTIKATDIGGKTTSINVEVNVLEYKTNDMNSSEF
                     KRMVSSEMYNLVNSYRKEKGKEPLQVSKNLEGLSNSWSKYMADKGQFSHVIDGKKSVE
                     VFTGYGLRSEENIAFVPLVTKSTYTTKDAREVANVIFTVWKKSDKYNENMLNSDFVYT
                     GFGLYILSNGEVYATQEFLNK"
     gene            3719..5899
                     /gene="slpA"
                     /locus_tag="PC523_002479"
     CDS             3719..5899
                     /gene="slpA"
                     /locus_tag="PC523_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HDF2475991.1"
                     /translation="MNKKNLAMAMAAVTVVGSAAPVFAADEQVKYQNTYTVVQSKYEK
                     ALKDMQKGITDKKIKSIAISYEGKPVTTITVADMDTKGKTSTKEELASALLKTTVNDK
                     LDNLGDGDYVDFDITYVGDADRLTAGDLNTFAKGIADSTEKKIPAAKGSNYGVAKTNS
                     GTGKLTTDTEAVISTSIEGKVEGNNLTISLKDAPSKVGVIGANNDTLADVTFADDAKL
                     TVSVGDPKIDLAKSFIFDTKTGKLGGIVEKENDATEHAYVRVINAKEQTIDLDASSYK
                     SAEDLAKAYAFDVNELKTLYTEIEAYQKDSNNKTDKVQIVDGKYQTILYAEGKRLTTK
                     SATQASKLADENSPLKLVIKADKLKDLKDYVEDLRNANNGYSNTVTVAGDDRIETAIE
                     LSSKYYNNSDEDNAITEDAVNNVVLVGSQAIVDGLVASPLASEKKAPLLLTSKDKLDS
                     NVKSEIKRVMNLKTTTGINNSKKVYLAGGVNSISKEVENELKDMGLKVTRLSGDDRYA
                     TSLEIADEIGLDDDKAFVVGGTGLADAMSIAPVASQLNEKGDATPIVVVDGKAKELSS
                     AAEDFLDDSQVDIIGGKNSVSKDMEDAIDDATGKSPNRVSGDDRQETNAEVLKESDYF
                     PDGAVNYFVAKDGSTKEDQLVDALAAAPVAANFGRTYNIKDNDSSGTVSPAPIILATD
                     SLSSDQNVAISKALPSGKSGDNLVQVGKGIANSVITKIKDLLDM"
     gene            6099..8444
                     /gene="secA"
                     /locus_tag="PC523_002480"
     CDS             6099..8444
                     /gene="secA"
                     /locus_tag="PC523_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDF2475992.1"
                     /translation="MSVLDTILDKADEQEIKKLNLIVDKIEALEEKIQNLSDDELKEM
                     TNVFRNRLNNGETLDDILMEAFAVVREVSKRKLGMRQYRVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVITVNDYLAERDKELMRPVYESLGMTVGVIIANQD
                     QNIRKQQYKCDITYGTNSEFGFDYLRDNMVPDLSHKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGDGDEDLKLYELANSFIKTIKEEDFEMDRKDKTIALTASGISKAESFFGITN
                     LTDIKNIELYHHINQALRGHKLMEKDVDYVISNGEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEINNESKTMATVTYQNFFRLYEKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNKPVIRADLHDKVFKTEEEKYNAVVEEIIRIHKTRQPILVGTVSVEKSEKLSRMLKK
                     QGIKHQVLNAKQHDKEAEIISKAGKLDAITIATNMAGRGTDISLGAGDREEEQQVKNL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKTIEKLMKR
                     TSSKENTAIESKALTRAIERAQKGVEGKNFEIRKNVLKYDDTINEQRKVIYNERNKVL
                     NNEDIQEDIQKMVKDIIQEAGENYLVGRKRDYYGYFKYLYSTFMPADTLLIPGVDKKS
                     VQEIVDSTYEISKRVYDLKKMMLGIDKVSELEKTVLLKVVDQYWIDHIDAMEQLRQYI
                     GLKSYAQKDPFKEYALEGYDMFEALNKNIREATVQYLYKFN"
     gene            8858..10729
                     /locus_tag="PC523_002481"
     CDS             8858..10729
                     /locus_tag="PC523_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HDF2475993.1"
                     /translation="MNRKNLSVIMATAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRDLMSQKYTGGSQVGQPIYEIKVGENLSKLKVITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEAIEKYENSADLSAEANSITEKAKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTGTVTSKTIEVGIGDEKVDLTANPIDSTGTNLDPSAEGFRVNKINK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVVNGTSKSISDISA
                     KDSETGKYKFTIKYTDASGKAIELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNVVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGYDRHTTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSSDAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKTDITDSQKSALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            10836..11543
                     /locus_tag="PC523_002482"
     CDS             10836..11543
                     /locus_tag="PC523_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2475994.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGTCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELNKEKAREIILKYLEEYPDAKVKTVTPFKASGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKRWNPESGHYAIGYHSVK
                     SHFDELATNKIQYVHAP"
     gene            11576..13408
                     /locus_tag="PC523_002483"
     CDS             11576..13408
                     /locus_tag="PC523_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2475995.1"
                     /translation="MKISKKIVSLLTMTFLTVTLYGNTSNASTKDTLTGSGRWETAIK
                     ISQAGWSKSDTAVLVNDNSIADALSATPFAKAKDAPILLTQSNKLDSRTKAELKRLGV
                     KNVYLIGGSIALSSEIEKQLNAENINFERISGNSRYDTSLKLAEKLNKEKSISKIVVV
                     NGEKGLADAVSVGAIAAQENMPIILSDSENGTEVADNFIDSKDIEKSYVIGGTYSISN
                     SVERNLPNATRIAGSSRSETNAKIIEEFYKDTDIKNIYVTKDGTRNKNDLIDSLAVGV
                     LAAKNSSPIVLAGNKLDTTQKDVLNTKVIDKVTQIGGLGNEDAVKSIIDMQEKTKYTI
                     ETIDELNVAIKKADANDVIIFEPEKDTSISDSFKIETNKAITVEFDGVFKKSITIDMP
                     NGDVKNFGEISDDIRIDNIKKGTLINEGSIQGIDIYSKNGCKIENTSDGDIWIITIDA
                     DAKDVYIENDGDITKISNNAPGVIIKNSGKIDLVNGNEQPAISGKKPTTNDTEYNDER
                     ARGLSVSTKPCSIPEKDRVRVTISSEPKSSRYKIYYRVVEDKPSAMYVGEKISVRSWD
                     LASKSDGSFVEKAKNGSYIEVVEINTSTNKVSRWGRSNVTDDGF"
     gene            complement(13455..13856)
                     /locus_tag="PC523_002484"
     CDS             complement(13455..13856)
                     /locus_tag="PC523_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDF2475996.1"
                     /translation="MILKKHKETILYLFFGAFTTLVNIVSYLFFTRVISFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIELRFLFKEFFSFVSFRLLSGVIEMVIMYIMIDLLF
                     VNDIIVKVFTNIVVIVLNYLFSKMIIFKNKK"
     gene            14084..16495
                     /locus_tag="PC523_002485"
     CDS             14084..16495
                     /locus_tag="PC523_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HDF2475997.1"
                     /translation="MRKYKSKKLSKLLALLTVCFLIVSTIPVSAENNKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRSKSRSSLPSSYKSSVAYNPMNLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATRPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHSVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETN
                     IDAYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKSELQRLGTKKVYLIGGEDSLSKNVQAQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKRQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKTVLKSKKIETPIRVGGNGNESAFNELN
                     TLLGK"
     gene            16798..18375
                     /locus_tag="PC523_002486"
     CDS             16798..18375
                     /locus_tag="PC523_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HDF2475998.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLVGNGRYETAVK
                     ISQKAYSSSSNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKGNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERLSGKDRNETNAKVIEKFYSDTSLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLSTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKEDTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKIN
                     KIQDILDKLGFLKKFLS"
BASE COUNT         7722 a         2077 c         3435 g         5349 t
ORIGIN      
        1 aatatcatat atactctata atatgtggaa aataaagaaa aaataaatgg aaaataatcg
       61 taatgtaaat ataaaatata aaaatcatca gggggatatg aaaatgaaca aaaaaatatt
      121 atcattaggt ctagcagtat cattaatctt agtaaacttc aaaagtgtaa atgcatcatc
      181 agtagtagaa aaaatatatg gtaaagatag atatgaaaca gcagcaaaga tagctgatag
      241 acagacttat gaaacagtaa ttttagtaaa tacagaaaaa tcacttgcag atggattgag
      301 tgcaagtgga ttatcaggtg taacaaaagc gccaatacta tttacacaaa aaaataagat
      361 accagcagac acaaatagat gtttaaaaaa tatcaaaaaa gcatacataa taggtacaga
      421 agatactata agtaaatcag tagaaaagga actaaattct aaaaatatag aagtcaaaag
      481 aattggtgga gaagataggc taaaaacaag ctatttaatg gctaaagaaa tagcaactat
      541 aaagaaagtg gataaagtac tattaactaa tgcatatagt ggagaagcag acgcaatgag
      601 tgtatcatca gtagctacta gagatggagc tccaatcata cttacagatg gaaagggtat
      661 cccttttgat gtcaaaaatg ttcagtcata ttgtataggt tcagaggaga taatgagtaa
      721 ctcattagtt aaaaatacaa actcggtaag aatagaagga actgacagat ttgaaaccaa
      781 taaaaatgta atagactact tttttaagaa tgcagatgga ttctatgtat cagatggata
      841 ccaattagta gatgcaatag cagctgcacc acttactaaa gactctccaa tggtgctagt
      901 aaatgatgga agtgataaga ctgtattaga aggagctaag aatataactt ctgtaggtga
      961 aatagatgag aaggtagtac aacaatgtac aaatgcatca aagtcaaatg gacaacctcc
     1021 aacaattaca ataggaaata tagagatata taagggtgaa aagtttgaca ctggcaaatt
     1081 aaatatagtg gctaaagata atactggaaa ggtattacca atagaagttg atgggtttat
     1141 agatacaaat agggtaggta catacatatt gacattaaaa gctacagatg aatggggaaa
     1201 aagtacagaa aaaagagtag aaataaaagt gttggatgat aaatctcatg attacaacag
     1261 cccagaattt aaaaaaatgg tatctacaga aatgtataac ttaatcaatt cctatagaaa
     1321 agaaaaaggc aaagagactt tgttagtgtc tagtaagtta gaagacatgg caaatgcatg
     1381 gtctaagtat atgatggata aaaaagtatt tgcacattat atagatggta aaaatgctcc
     1441 acaagtattt tctgagtttg gaatgagaag tgaggaaaat atagcatata tttatataga
     1501 ttctaagaag gttcaaacaa ctcaagatgc aaaagatttg gcaaaggcta tatttgaggt
     1561 gtggaagaag tctccagagt ataatgcaaa tatgcttagt gatgagttct atagtactgg
     1621 atttgggctt tatatattat ctgatggtca agtgcatgct actcaagagt ttttaaatgg
     1681 caatgaaggt agcttgtaaa aaattccata aatgttgttt ttttgttaat ttactgatat
     1741 tataaaatca aaatcacatc taaagtactt tttgtaatct gttttgttat tgctttatag
     1801 gttatggtat tttagatgtt ttttgtttta gtagtttgta aattttgagg gaatcactac
     1861 tggggatgta atttatgatt agctgaatta tataaaaaat tagtaggagg agttcgatga
     1921 aggtaaataa gagaatatta tcgataggat tgacaatatc attaataatg gcaggagcac
     1981 caaatatcaa tgcactttca agtatagaaa agatacaagg gaaagataga tatgagacgg
     2041 ctgccaaaat tgcacagaag caaacgtatg aaaatgtagt attagtaaat acagataata
     2101 cactagctga cggactaagt gcaagtggat tagcaggagc agttaaagca cctatattat
     2161 tatcacaaag aaataatata ccaccagaca cagaaaaaat gttaaaagat gtaaagaagg
     2221 cttatataat aggaactgaa gattcaatag gaaaatcagt tgaaaatgaa ttaaagcaaa
     2281 aagggataga agttaaaaga ataggtggtg atgatagaat agaaacaagt tatcttatag
     2341 caaaagagat agcttcaata aagtctataa ataaggtttt tataactaat ggttatacag
     2401 gtgaagcaga tgctatgagt gcgtcatcag tagcatcaag agatggtgca cctataattt
     2461 taactgatgg taaaaatgtt ccatttgaga aaaaagaagg tgtgcaatgt tatgctctgg
     2521 gttcagagga gataataagt aatgatttag ttaaaaaaac taatgcagtt agattagctg
     2581 gagaagatag atttgagact aataaaaaag ttataaaaca tttttatagt tcagcaaaag
     2641 aattttatct ttctaaaggt taccaattag tagatgcagt tgctggttct tctatagcta
     2701 aaaatgcacc tattgtattg gttgatggaa atagtgataa gtctgtgctt agaagtgctg
     2761 ataaaatcac agcattagga ggaatagatg aaaagacact agaacaatgt ttaagtgcat
     2821 catcactgga tgcaagtgca ccaacaataa ctgttggaaa tttaaatatt tatcaagggg
     2881 ataagtttga tataagtaaa cttaatatat tagcaaagga tagcaatggt aacgatttga
     2941 caccagagct tataggaaat attaatactg ataaggttgg taaatacaaa gttactataa
     3001 aagctactga tattggtgga aagactacat caataaatgt agaagtaaat gtacttgaat
     3061 ataagactaa tgatatgaat agtagtgagt ttaaaagaat ggtttctagt gaaatgtata
     3121 atttggtaaa ttcatataga aaagaaaaag gtaaagaacc attacaagtt tctaaaaact
     3181 tagaagggct ttctaattcc tggtctaagt acatggcaga taaaggccag ttttcacatg
     3241 taatagatgg taagaaatct gttgaagtat ttactggata tgggttgaga agtgaagaga
     3301 atatagcttt tgtaccactt gttacaaaaa gtacttatac gacaaaggat gctagagaag
     3361 tagcaaatgt aatatttaca gtttggaaaa agtcggataa atataatgaa aatatgctaa
     3421 atagtgattt tgtatatact gggtttggac tgtatatatt gtctaatgga gaagtgtatg
     3481 ctactcaaga atttttaaat aagtaaaatt aaatttttta agttattaaa tattgaaatt
     3541 taagggtata aaaactcata tacttgggaa aaataataat ttcaaaaaaa tgccttgcaa
     3601 aaagaattaa aatggattat tatagagatg tgagaaatat taggaatata tggatgatta
     3661 ttctatgtac ataataaaga gatgtaattt taatataatg ttgggaggaa tttaagaaat
     3721 gaataagaaa aatttagcaa tggctatggc tgctgttaca gtagtaggtt cggctgcacc
     3781 agtatttgca gcagatgaac aagttaaata ccaaaacaca tatactgtag tgcaaagtaa
     3841 atatgaaaaa gctttaaaag atatgcaaaa ggggattaca gataaaaaga taaaatcaat
     3901 agcaatatct tatgaaggta agccagttac tactattact gttgcagata tggatacaaa
     3961 aggaaaaact tcaacaaaag aagaattagc aagtgcttta ttaaagacta ctgttaatga
     4021 taagttagat aatttgggtg atggagatta tgtagatttt gatataactt atgttggtga
     4081 tgctgataga cttactgcag gggatcttaa tacttttgca aaaggtatag cagatagtac
     4141 tgaaaagaaa attcctgctg ctaaaggttc taactatgga gttgctaaaa caaattctgg
     4201 aactggaaaa cttactacag atacagaggc agttatatct acttcaatag aaggtaaagt
     4261 tgagggaaat aatcttacaa taagtcttaa ggatgctcca agtaaagttg gtgtaatagg
     4321 tgctaataat gatacacttg cagatgttac atttgcagat gatgcaaaat taactgtatc
     4381 agttggagat ccaaaaatag atttagcaaa atcatttatt tttgatacta aaactggtaa
     4441 attaggtgga atagtagaaa aagaaaatga tgcaactgaa catgcatatg taagagtaat
     4501 aaatgctaaa gaacaaacaa tagatttaga tgctagttca tataaatcag ctgaagattt
     4561 agctaaagct tatgcatttg atgtaaatga gcttaagact ctttacactg aaatagaagc
     4621 atatcaaaaa gatagtaata ataaaactga taaagttcaa atagttgatg gtaaatatca
     4681 aacaatactt tatgctgaag gaaagagatt aactactaaa tcagcaactc aagcttctaa
     4741 attagcagat gaaaactcac cacttaagtt agttataaaa gcagataaat taaaagattt
     4801 aaaggattat gttgaagatt taagaaatgc taataatggt tactcaaata ctgttactgt
     4861 agcaggtgat gatagaatag aaactgcaat agaattaagt agtaaatact ataataattc
     4921 agatgaagac aatgctataa cagaagatgc tgttaataat gttgtattag ttggatctca
     4981 agcaatagtt gatggacttg ttgcatcacc tttagcttca gagaagaaag ctcctttatt
     5041 attaacttca aaagataaat tagattcaaa tgtaaaatct gaaattaaaa gagttatgaa
     5101 tttaaaaact acaacaggaa taaacaattc taagaaagtt tacctagctg gtggagttaa
     5161 ttctatatct aaagaagttg aaaatgaatt aaaagatatg ggacttaaag taacaagatt
     5221 atcaggagat gatagatatg ctacttcatt agaaatagct gatgaaatag gtttagatga
     5281 tgataaagca ttcgtagtag gtggaacagg acttgcagat gctatgagta tagctccagt
     5341 tgcttctcaa ttaaatgaaa aaggtgatgc tacaccaata gttgtagttg atggaaaagc
     5401 taaagaatta agctcagcag ctgaagattt cttagatgat tcacaagttg atataatagg
     5461 tggtaaaaat agcgtttcta aagacatgga agatgctata gatgatgcta caggaaaatc
     5521 tccaaataga gttagtggag atgatagaca agaaactaat gcagaagtat taaaagaatc
     5581 tgattatttc ccagatggtg cagtaaatta ctttgttgct aaagatggat ctacaaaaga
     5641 agaccaatta gttgatgcat tagcagcagc accagtagca gccaactttg gaagaactta
     5701 taatataaaa gataatgata gttctggaac agtttctcca gcaccaatca tattagcaac
     5761 tgattcatta tcttcagatc aaaatgttgc aataagtaaa gcattacctt ctggaaaatc
     5821 aggagataat ttagtacaag ttggtaaggg tatagcaaac tcagtaatca ctaagataaa
     5881 agacttatta gatatgtagt ataaatttaa aatacaaagt aaaaaagatt cctattttag
     5941 gaatcttttt tactttgtat tttaaaaaat acctaactga ttaagagatg taattcattc
     6001 aagtatgatt attatactat aaataaaaaa gttgtctact attccacatt tttaaggtat
     6061 aataatagtt gtatgataaa aaaggaggta agaaacacat gtcagtttta gatacaatac
     6121 ttgataaagc agatgaacaa gaaattaaga agttgaattt aatagtagat aaaatagagg
     6181 ctttggaaga aaaaatacaa aacttatctg atgatgaatt gaaagaaatg actaatgtat
     6241 ttaggaatag attaaataat ggagaaactt tagatgacat attaatggaa gcttttgcag
     6301 ttgtaagaga agtatcaaaa agaaaattag gaatgcgtca atatagagta cagttaattg
     6361 gtgggatagt aatacatcaa ggtaaaatcg ctgagatgaa aacaggtgaa ggtaaaacgt
     6421 tagttgaagt agcaccagta tatctaaatg ctcttacagg taagggtgta catgtaatca
     6481 cagtaaatga ttacctggca gaacgtgata aagaacttat gagaccagtt tatgaatctc
     6541 ttggtatgac agtaggagta attatagcta accaagacca aaatataaga aaacaacagt
     6601 ataaatgtga tataacttat ggtacaaata gtgaatttgg atttgattat ttaagagata
     6661 atatggttcc agatttatct cataaggtac aaagagaact aaactttgcc atagtagatg
     6721 aggtagactc aatattaata gatgaagcta gaactccgct tattattgca ggagatggag
     6781 atgaagattt aaaactttat gaattggcaa atagctttat aaaaactatt aaagaagaag
     6841 actttgagat ggatagaaaa gataaaacta tagcattaac agcaagtggt ataagcaaag
     6901 ctgagtcatt ttttggtata acaaacctta ctgatataaa gaatatagaa ttatatcatc
     6961 acataaatca agctttaaga ggtcataagc ttatggaaaa agatgttgac tatgttatct
     7021 caaatggaga agtaatgata gttgacgaat ttacaggaag agtaatggat ggtagaagat
     7081 atacagatgg acttcaccaa gctatagaag caaaagaagg tgttgagata aataatgaat
     7141 ctaaaactat ggctactgtg acttatcaaa atttcttcag actatatgaa aaactttctg
     7201 gtatgactgg tactgcaaag acagaagaag gggaatttga gtcaatctat aaactaaatg
     7261 ttgttcaaat accaactaat aaaccagtga ttagagctga tttacatgat aaggtattta
     7321 agacagaaga agaaaagtat aatgctgttg tagaagaaat aataaggata cataagacta
     7381 gacaaccaat acttgtagga acagtttctg ttgaaaaatc tgaaaaatta tctagaatgc
     7441 ttaaaaaaca aggtattaag catcaagtct taaatgcaaa acaacatgat aaagaggcgg
     7501 agataatttc taaagctggt aaattagatg ctataacgat tgctacaaat atggcaggta
     7561 gaggaacgga tatttctcta ggtgcaggag atagagaaga agaacaacaa gttaaaaatt
     7621 taggtgggct ttatgttata ggaacagaaa gacacgaatc aagaagaatt gataatcagc
     7681 ttagaggacg ttctggtcgt caaggagacc caggtacatc aagattcttt gtaagtcttg
     7741 aagatgatgt aataaagctt tatggtggaa aaactataga gaaacttatg aagagaacaa
     7801 gttcaaagga aaatactgct attgaaagta aagcacttac aagagctata gaaagagctc
     7861 aaaaaggtgt agaaggtaaa aattttgaaa taagaaaaaa tgtccttaaa tatgatgata
     7921 ctattaatga acaaagaaaa gttatatata atgaaagaaa taaagtttta aataatgaag
     7981 acattcaaga agatattcaa aaaatggtta aagatatcat acaagaagca ggagaaaatt
     8041 atttagttgg aagaaaaaga gattattatg gatattttaa atatctatat agtacattta
     8101 tgccagcaga tacactatta atacctggag tagataaaaa aagtgttcaa gaaatagttg
     8161 atagtactta tgaaatttca aaaagagttt atgatttaaa aaagatgatg cttggtattg
     8221 ataaggtttc agagttggaa aagacagtac ttttaaaagt agttgaccaa tactggatag
     8281 accatataga tgctatggaa cagttaagac agtatatagg tcttaaatct tatgctcaaa
     8341 aagacccgtt taaggaatat gctttagaag gatatgacat gtttgaagct ttaaataaaa
     8401 atataaggga agcaacagtg caatacttat ataaatttaa ctaaaaaagt gcatataatt
     8461 tttattaaat gaaaaataaa gtattttgat tctttatatt gtaattacaa ttaaataatt
     8521 aaatatataa atgaaaaaga agctgaaaat cagcttcttt ttctgtgtga gaaatacgga
     8581 aaaaagagat gcttgaaatt tctttcttcc atctaatatt atactataaa gaagatgaaa
     8641 agtctattag ttataaactg gattattaaa aaaaattaca gttttgttac ttttttgtat
     8701 acataaatat taaaaattta aagtattaga aaaaatttat atcttttggt taattattac
     8761 aataagtctc atttattgaa ataatatcaa atatatatta taattggtaa ataaggaaaa
     8821 aatgataaaa aattgaattt tttaggggga aaataccatg aatagaaaaa atctttctgt
     8881 aattatggct actgcaatga taagtacatc agtagctcca gtttttgctg cagaaactac
     8941 acaggtaaaa aaagaaacaa taactaagaa agaagctaca gaactagttt caaaagttag
     9001 agatttaatg tctcaaaagt atacaggagg ttctcaagtt ggacaaccaa tatatgaaat
     9061 aaaagttggt gagaatttat caaaattaaa agtaataact aatatagacg aattagagaa
     9121 attagtaaat gctttgggag aaaataaaga acttattgta actataacag ataaagggca
     9181 tataacaaat agtgcaaatg aagtagttgc agaagcaatt gaaaaatatg aaaattcagc
     9241 agacctttcc gctgaagcta attctataac agaaaaagct aaaactgaaa ctaatggaat
     9301 ttataaagtt gcagatgtaa aagcttcata tgatagtgct aaagataagt tagttataac
     9361 tttaagagat aaaacaggaa cagtaacttc taaaactata gaggtaggta ttggtgatga
     9421 aaaagttgat ttaacagcaa atccaattga ttcaactgga acaaacttag acccttctgc
     9481 agaaggattt agagtaaata aaatcaataa actaggtgta gcaggagcta aaaatattga
     9541 tgatgttcaa ttagctgaaa taactataaa aaatagtgac ctaaatacag tttcaccaca
     9601 agatttatat gatggatata gattaactgt taaaggtaat atggtagtaa atggtacatc
     9661 aaagtcaatt agtgatattt cagcaaaaga ttcagaaaca ggaaaatata aatttactat
     9721 taagtatact gatgcatctg gaaaagcaat agagcttact gtagaaagca ctaatgaaaa
     9781 agatttaaaa gatgccaagg ctgcattaga aggtaattca aaggttaaat tgatagctgg
     9841 agatgataga tatgcaactg cagtggctat agcaaaacaa acaaaatata ctgacaatgt
     9901 agttatagtt aattcaaata aactagttga cggattagca gctacaccac tcgctcaatc
     9961 taaaaaagca cctatattgt tagcatctga taatgaaata ccaaaagtaa ctttagatta
    10021 tataaaagat ataattaaga aaagcccatc agctaaaata tatatagtag gtggagagtc
    10081 agcagtatca aatacagcta aaaagcaatt agaatcagta actaagaatg ttgaaagact
    10141 agctggatat gatagacata cgacttctgt agcagtagca aaagctatgg ggtcttttaa
    10201 agatgcattt gtagtaggtg caaaagggga ggctgatgct atgagtatag ctgctaaagc
    10261 tgctgaactt aaggctccta taatagtaaa tggctggaat gatctttcat cagatgctat
    10321 caaactgatg gatggaaaag agattggcat agttggtggt tctaacaatg tatctagtca
    10381 aattgaaaat caacttgctg atattgataa agatagaaaa gttcaaagag ttgaaggaga
    10441 aacaagacac gataccaatg ctaaagttat agaaacatat tatggaaaat tagataaact
    10501 atatatagca aaagatggat atggaaataa tggtatgcta gtagatgcat tggcagcagg
    10561 acctctagca gcaggtaaag gtccaatact tctagctaaa actgatataa cagactcaca
    10621 aaagagtgca cttagtaaaa aattaaatct tggtgcagaa gtaactcaaa taggtaatgg
    10681 agttgaattg acagtaatac aaaagatagc taaaatacta ggttggtaat aacttatact
    10741 gtggagaaga ttccttaata aatacaggaa tctttttcta taactatgct atttattgta
    10801 aattataaca ttttatgaag taggaggatg tagtagtggt agatgcaact ttatttttta
    10861 caccacatca agatgatgaa actttaagta tgggaagtgc tataattgaa catgttgaaa
    10921 aaagtgatac acatgtcata ttatgtacag atggtagtaa atctataata agaaaagttt
    10981 tggatgatgg aggcacatgt tcttatcata ttaaagatgt acataaatat tctttatcag
    11041 aaagtgaatt ttcaaaagat agagatgaag aatttaagga tagttgtgaa gcaatgggtg
    11101 taaaagaaag taatatacat atagaagata atagagccca tgatggcgag ttaaataaag
    11161 aaaaggcaag ggaaataata cttaagtact tagaagaata tccagatgca aaagtaaaaa
    11221 cggttactcc gtttaaagct agtggaatac atgaagacca tagagcatta ggagaagctg
    11281 cactagagtt atatagagaa ggaaaaataa aagatttaag attttatgta gaaccatatg
    11341 actataagga ttttaaaaaa gttaatccaa atgtagaggt atggaaggta ttacctagtc
    11401 aagaagagaa gttattaagt gcaatgaatg catataagag atggaatcct gaaagtggac
    11461 attatgctat aggataccat tcagtaaaat ctcattttga tgagttagct acaaataaaa
    11521 tacagtatgt acatgctcca taaaatatta cgaaagaatt aggaggtaag aattaatgaa
    11581 aatatcaaaa aagatagtgt ctttgttaac tatgacattt ttaactgtta cattatatgg
    11641 aaatacatct aatgcatcta caaaagacac attaacaggt tctggaagat gggaaacagc
    11701 aataaaaata agtcaagctg ggtggtcaaa gtctgataca gctgtattag taaatgacaa
    11761 ttccatagca gatgctttat cagctactcc atttgcaaaa gcaaaagatg caccaatatt
    11821 attaactcaa agtaataaat tagatagtag aaccaaagca gaattaaaaa gacttggtgt
    11881 aaaaaatgta tatttaatag gaggttcaat tgcattaagt tcagagattg aaaagcaatt
    11941 aaatgcggaa aatataaatt ttgaaagaat atctggaaat agtagatatg atacttcttt
    12001 aaaactagct gaaaagttaa ataaggaaaa atctatctct aaaatagtag tagtaaatgg
    12061 agaaaaggga cttgctgatg cagtaagtgt tggagctata gctgctcaag aaaatatgcc
    12121 aataatactt tctgattcag agaatggaac tgaagtagct gataatttta tagatagtaa
    12181 agatatagaa aaatcgtatg taataggtgg tacatattct atttctaatt ctgtagaaag
    12241 aaatttacca aatgcaacaa gaatagcagg cagtagtaga agtgaaacaa atgcaaagat
    12301 tatagaagaa ttttataaag atactgacat aaaaaatatt tatgttacaa aagatggtac
    12361 aagaaataag aatgatttaa tagattcttt agcagtgggt gtattagcag ctaaaaatag
    12421 ttctccaata gtattagcag gaaataagct tgatactact caaaaagatg tgttaaatac
    12481 taaggttata gataaagtta ctcaaattgg tggtttaggg aatgaagatg ctgtaaaaag
    12541 cataatagat atgcaagaga agactaaata tactatagaa actattgatg agttaaatgt
    12601 tgctataaaa aaggcagatg caaatgatgt aataatattt gaaccagaga aagatactag
    12661 tataagtgat tcattcaaga tagaaacaaa taaagctata actgtagaat ttgatggagt
    12721 atttaaaaaa tctataacta tagacatgcc taatggagat gtaaagaatt ttggagaaat
    12781 ttcagatgac ataagaatag ataatataaa gaaaggtact ttgattaatg aaggtagtat
    12841 acaaggtata gatatttatt ctaagaatgg gtgtaagata gaaaatacaa gtgatggaga
    12901 tatatggatt ataactatag atgctgatgc taaagatgta tatatagaaa atgatggaga
    12961 cataacaaaa atatcaaata atgctccagg tgttataata aaaaattcag gaaagattga
    13021 tttagtaaat ggtaatgagc aacctgcaat tagtggaaag aaaccaacaa ctaatgatac
    13081 tgaatataat gatgaaagag cgcgtggatt gtctgttagt acaaagcctt gctcaatacc
    13141 tgaaaaagat cgtgtaagag tgacgatatc tagtgaacct aagagttcaa gatataaaat
    13201 ttattataga gttgtagaag ataaaccttc tgctatgtat gtaggagaaa aaattagtgt
    13261 aagaagttgg gacttagcat caaaatcaga tggatctttt gtagaaaaag ctaaaaatgg
    13321 atcatatata gaagttgttg aaataaatac ctcaactaat aaagtttcta gatggggaag
    13381 atcaaatgta actgatgatg gattttaaat caccttaaat aaagttaaaa aagtagatta
    13441 ctatttctag tagtctactt tttattttta aaaattatca tcttactaaa caaataattt
    13501 aaaactataa caactatatt agtaaatact tttacaataa tatcatttac aaaaagtaaa
    13561 tcaatcatta tatacataat aaccatttct ataacaccag aaagtagcct aaaactaaca
    13621 aaagaaaaaa attccttaaa caaaaatctt agttcgattc ttttactttc aaacacaaaa
    13681 aacttattag taacataagc aaataaaaca gcaagtatcc atgccaaagc atttgcaacc
    13741 ataaaattaa atgaaataac tctagtaaaa aataaatacg aaactatatt tacaagagta
    13801 gtaaatgctc caaaaaataa atataatatt gtctccttat gcttttttaa aatcaaatat
    13861 aatcctccta agctaattta atataaatat acttaaagat actaacaaac attagtatag
    13921 cacatctatc aataaatcaa tatatcttaa aatattataa aaaataaaac tataatattc
    13981 tataattaat acgtactcat aatccatatt ttatcaaaat aaaatatgac cttaatttag
    14041 aaatctgata taataaaaat taaatacata taagggggta aacatgagaa agtacaaatc
    14101 aaaaaaattg tcaaagctac tggcattgtt gacagtttgc tttttaatag tgtcaacaat
    14161 acctgtctca gcagaaaaca ataaaactct agatggagta gaaactgcag agtattctga
    14221 aagctatctt caatacctag aagatgtcaa aaatggagac acagcaaaat ataatggagt
    14281 aatacctttt ccacatgaaa tggaaggtac aacacttcga agtaagagta gaagtagtct
    14341 tccatcatca tacaaatcaa gtgtagcata caacccaatg aatcttggtc ttactacacc
    14401 agcaaaaaat caaggaagtc ttaatacatg ctggtctttt tcaggtatgt caactttaga
    14461 agcatatctt aaattaaagg gatatggaac gtatgactta tcagaagaac atttaagatg
    14521 gtgggcaact ggtggaaaat atggatggaa cttagatgat atgtcaggta gttcaaatgt
    14581 aacagccata ggatatctga cagcatgggc aggtcctaag ttagaaaaag atataccata
    14641 taatcttaaa tctgaggcgc aaggtgcaac tagaccttca aatatggata ctgcacctac
    14701 acaatttaat gtaacagatg ttgttcgtct taataaagat aaggaaactg taaaaaatgc
    14761 tataatgcaa tatggttcag tgacatcagg ttatgcacat tattcaacat attttaataa
    14821 agatgaaaca gcatataact gtacaaataa gagagctcca ttaaatcact ctgtagcgat
    14881 agtaggatgg gatgataatt attcaaaaga taactttgca tctgatgtaa aaccagaatc
    14941 aaatggagca tggttagtaa aaagcagttg gggagagttt aattctatga aaggattctt
    15001 ctggatttct tatgaagata aaactctact aacagataca gataactatg caatgaaatc
    15061 agtatcaaaa ccagatagtg ataaaaaaat gtaccaactt gaatatgctg gtcttagcaa
    15121 gataatgtca aataaagtaa cagcagcaaa tgtatttgat tttagcagag attctgaaaa
    15181 acttgattct gttatgtttg aaacagactc tgtaggagca aaatatgaag tatattatgc
    15241 accagtagta aatggagttc ctcaaaataa ttcaatgaca aaacttgcaa gtggaacagt
    15301 atcatattct ggatacataa atgtacctac taattcttac agcttaccaa aaggtaaagg
    15361 agcaatagta gtagttatag acaacacagc aaatcctaat agagaaaaat caactttagc
    15421 atatgaaact aatatagatg catattattt atatgaagct aaagcaaact taggtgaaag
    15481 ttacatactt caaaacaata aatttgaaga tataaataca tatagtgaat tttctccttg
    15541 taactttgtt ataaaagcta taacaaaaac atcttctgga caagctactt caggagaatc
    15601 tttaactgga gcagatagat atgaaacagc agttaaagtt agtcaaaaag gatggacttc
    15661 ttcacaaaat gcagtattag taaatggaga cgcaatagtg gatgctttaa cagctacacc
    15721 atttacagca gcaatcgact ctccaattct tttaacagga aaagataact tagattcaaa
    15781 aactaagtca gagttacaaa gattaggaac taaaaaagtt tatctaatag gtggagaaga
    15841 ttctttaagt aagaatgtac aagctcaact tagcaatatg ggtatatcag tagaaagaat
    15901 ttcaggtagt gatagataca agactagtat atctctagct caaaagctaa atagtataaa
    15961 atctgtttca caagttgcag tggcaaatgg tgtaaatgga cttgcagatg caataagtgt
    16021 tggtgcagca gcagctgata acaatatgcc aataatactt actaatgaaa agagtgagtt
    16081 gcaaggtgct gatgagtttt taaattcatc aaaaataact aagtcttata taattggtgg
    16141 cacagctact ttatcatcaa atttagaaag taagctttca aatccaacaa gacttgcagg
    16201 aagtaataga aatgaaacta atgctaagat aatagataaa ttctacccta gttcagattt
    16261 aaaatatgct tttgttgtta aagatggttc aaagcgtcaa ggagatttga tagatggact
    16321 agcagtagga gcattaggtg ctaaaactga ttcaccagta gttctagttg gaaataagtt
    16381 agatgaaagt caaaaaactg tacttaagtc taagaaaata gaaactccta ttagagttgg
    16441 tggaaatgga aatgaaagtg cttttaatga actaaatact cttttaggaa aatagtgaat
    16501 agaaagatag taaggatata cattttatta ttaaaaaata ttcaatagaa gtttataaat
    16561 ataaaataac aaaaaggggt gtctcaaaat gaacttttta gtccatgagg cactcttttt
    16621 tgtatgaatt caaatatatt ttcatcattt caacaatgga aaataggatt gtctatattt
    16681 tgagatagcc tctttattat tgatgaaaaa tgtaataatt ttaaactgta aaatagtgta
    16741 aagttatttt ataatataaa tatagtaaga ttttaaacaa aataaggagg aataataatg
    16801 aaagcaccaa aaactatttt aacaatactg acaatagcgc ttactttaag tagcatttct
    16861 ataataccat catatgcact tacagaggaa aaattagtag gtaatggtag atatgaaact
    16921 gctgtaaaga taagtcaaaa ggcatacagt tctagttcta atgtagtgtt agttaatgat
    16981 aattcattag ctgatgcact ttcagctaca ccatttgcaa aggctaaagg agcaccaata
    17041 cttttgacag aaagtgataa actagatgat agaactgaaa aagagataaa acgtctagga
    17101 gctaaagata tttatttaat aggaggtact gctgtactta ataaagatat agagaacaaa
    17161 ttaaaaggta atggacttaa tgtagaaaga attaatggta aggatagata tgaaacttca
    17221 ctaattttgg ctaataagct taaggatata aaagatatta aagaagttgc tgtagtaaat
    17281 ggtgaaaaag gactgagtga tgctgttagt gttggagcac cagcagctca aaataagatg
    17341 cctataattt tgtctagtcc taaggatgga gtagaagcat ttgataaatt tataagagat
    17401 gagaaagtta taaaagcata tgtaattgga gggacaaatt cagtttcaag agctgtagaa
    17461 aagagtcttc ctaatgcaga aagattaagt ggaaaagata gaaatgagac taatgctaaa
    17521 gttatagaaa agttttattc tgatacaagt ttaagtaatc tatatgtaac taaggatgga
    17581 agcaaaaatg aaaatcaact gatagactca ttagcagttg gagtgctggc tgcaaaaaat
    17641 gaatctcctg ttgttttggt tggcaataaa ttaaacacaa aacaaaggga tatacttagt
    17701 actaaaaaat taagtactat aacacaggta ggtggaaatg gaaatgaaga agcttttgat
    17761 gagattaaaa gtttacaaga aaaaactgtg tttgaagcaa aaactgttga ggaattaact
    17821 gatatgataa atatagcaag tcctaatgac attataaatt ttaaaccaaa agaagatact
    17881 gtaaatgaag cttttagaat ggtgacaaat aaaccaataa ctgtgaatat aaaaggagat
    17941 tgttcaaaaa ctcttacagt agatatgcct aatggtgaag tgaataacta tgctactttg
    18001 gtaaatgtta tagttagaaa tattggagaa ggtggattta ataatcatga tacaatcacc
    18061 atattgtcag ttcgtgacaa aaatggtaga gttatagaaa atactagaaa ttcagatata
    18121 gatacgttga tgatattagc ttctgctaat gatacaaaat tgattaatga tggatatata
    18181 ggaaagctta tagataattc atctaatagt gatataacta ataatggtac tatagataaa
    18241 aaagttaatc aggttgagga tttagaggct aaggttgatt caattgaaaa agctatagat
    18301 tctataagtc agaaaatcaa taaaatacaa gatatactag ataagcttgg atttctaaag
    18361 aaatttctta gctaaaaata tgtatattta agtgaactgt gtgttggaat tatatctgaa
    18421 gatatatagt taaatgggtt tatagataaa taggcttata gctaaatata tagttcaata
    18481 tatagttaaa taaatcttgg ggaggtaaca tggggagcgg aattgaatgt aacaaaagaa
    18541 tacataaaag tattgacaaa actagaataa agaaaaataa tct
//
LOCUS       DAMJVM010000067        18575 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.070, whole genome shotgun sequence.
ACCESSION   DAMJVM010000067 DAMJVM010000000
VERSION     DAMJVM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18575
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.070"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            215..1432
                     /locus_tag="PC523_002487"
     CDS             215..1432
                     /locus_tag="PC523_002487"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HDF2475999.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKSYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDRYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKVVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1527..1907
                     /locus_tag="PC523_002488"
     CDS             1527..1907
                     /locus_tag="PC523_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2476000.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2193..2675
                     /locus_tag="PC523_002489"
     CDS             2193..2675
                     /locus_tag="PC523_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2476001.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNCINIEKVNDSVFSKRLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVNLQGDGFKNEVSQGD
                     NVKKGSPIISFEREKINSQGIDCTIIMVVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2696..3511
                     /locus_tag="PC523_002490"
     CDS             2696..3511
                     /locus_tag="PC523_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2476002.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKANEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEVIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSINLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNIYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3564..5114
                     /locus_tag="PC523_002491"
     CDS             3564..5114
                     /locus_tag="PC523_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2476003.1"
                     /translation="MKDKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     FPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQIAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGVDTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEGQSKQLNLDTGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEIY"
     gene            5161..6513
                     /locus_tag="PC523_002492"
     CDS             5161..6513
                     /locus_tag="PC523_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2476004.1"
                     /translation="MVITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKGYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLENFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6644..7402
                     /locus_tag="PC523_002493"
                     /pseudo
     CDS             6644..7402
                     /locus_tag="PC523_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7449..8168
                     /locus_tag="PC523_002494"
     CDS             7449..8168
                     /locus_tag="PC523_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF2476005.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKKWLELDDFYKIKSIYEFV
                     QNDILFGYNASDILSATQVLNDGMGQCNTKATLLMALLRAVNILCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFSNYGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8292..9692)
                     /locus_tag="PC523_002495"
     CDS             complement(8292..9692)
                     /locus_tag="PC523_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDF2476006.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADVGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLVARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLLLSPIAILDVILSFILLKNVGETQKLKLDIPSIILSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPIFIGIISLIFFTLRQLKSREPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPVAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLLHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINSTLRQISGAIGSAVLITIMTSATKKAHMSSAILSNIHGIDVAFSIAATLAFVG
                     LIVSICFIKRHQIIRS"
     gene            complement(9696..10604)
                     /locus_tag="PC523_002496"
     CDS             complement(9696..10604)
                     /locus_tag="PC523_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2476007.1"
                     /translation="MKEGNKVTEKQRLIITIAQKIFDQKGFQNTSISDIVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWENIFSRT
                     TINTYIRENFSEVQRKTTSKLQTKSRANLLNEYKLSLFDNYGDKIENIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFVAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEAYF
                     SLYNKSLFFKTIQDIEELIKTDTSLLENSKLLEATKKLYVEGKNQQYDSLIMDSMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            10866..11513
                     /locus_tag="PC523_002497"
     CDS             10866..11513
                     /locus_tag="PC523_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2476008.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARNAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGNDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKEHITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11838..14477
                     /locus_tag="PC523_002498"
     CDS             11838..14477
                     /locus_tag="PC523_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDF2476009.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQTLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGSVVVYGRGSFIVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMVGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEDDFVPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     DGDLALTGKELDALSEEELYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSILFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLRAVAIRGCIIGIVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14535..15638)
                     /locus_tag="PC523_002499"
     CDS             complement(14535..15638)
                     /locus_tag="PC523_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HDF2476010.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFLTLDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVNPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQVIDNIE"
     gene            15999..16820
                     /locus_tag="PC523_002500"
     CDS             15999..16820
                     /locus_tag="PC523_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2476011.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKNGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTTTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIIRDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    16900..17110
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17197..18513
                     /gene="argH"
                     /locus_tag="PC523_002501"
     CDS             17197..18513
                     /gene="argH"
                     /locus_tag="PC523_002501"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDF2476012.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCILYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLALDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVDLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELYKFSDAFKEDIYDFIDYESILNKGIKKNLK"
BASE COUNT         7110 a         2146 c         3280 g         6039 t
ORIGIN      
        1 atgagaatgt aaaaaatatt tttaaattat catttaatgt aacttaaatg aattgaagat
       61 atatgtttat tgtattcaat tgttgtattt ttaggttttt tgtgattaat taaaaaatta
      121 taaaaatcgt ttacatgttg atttttaaaa aaaaaagata tagaattata tagaaagtaa
      181 taaacaaaaa ttggatttac gggagtggaa tgaaatgatg aatgttacct taacagatgt
      241 gaaagaagct agagagacca ttaagaacat agtaaaaaga actgacttac ttgaaagtgt
      301 taaacttagc gaaaaaacag gagcaaatgt gttctataaa tgtgagaatc ttcaaaaaac
      361 aggttctttc aaattaagag gagcttgtaa caaaatagca agtttgacag atgaagaaaa
      421 ggcaaatgga gttatagctt cgagtgcagg gaatcatgct caaggagttg cattgggggc
      481 aaaaatgact ggtataaaat ctacaatagt aatgccagca actgcacctt tagcaaaagt
      541 tagtgcaact aaaagttatg gggcagaagt agttttaaat ggtgcggttt atgatgatgc
      601 atatgcaaaa gctgttgaaa tacaaaaaga aacaggagca actttcttac atccattcaa
      661 tgatagatat gttatagcag gacaaggaac tatatctctt gaaattttcg aacaattaga
      721 caataaagtt gatactatac tatgcccagt tggtggagga ggaatcatct ctggtgttgc
      781 tgttgcagca aaagcattaa atcctaatgt aaaagtagtt ggtgttcaaa ctgcaaatat
      841 accttcaatg aaagagtcaa taaaaaatgg aaaagtaaca acagcattta atgatactac
      901 aatagctgat ggtatagctg ttaagactcc aggagattta acttttgaga taataaatga
      961 attggttgat gaaatagtag tggttgaaga gacagaaatt gcagaatcaa tattatttat
     1021 gatggaaagt caaaaaatag tttcagaagg tgctggggct gtatgtacag ctgctatatt
     1081 aagtggtaag tatgtgccag caaaagatga aaatgtggtt tgtataatat ctggtggtaa
     1141 tatagacata aatactttat atagaataat aggtgttgct ttagctaaag aaggaagaag
     1201 atattcattt agtacaatta tggaagataa gccaggaaac tttgctgagt taactagaat
     1261 aataagtgag aatggtggaa acattttaag tgctaatcaa ggtaaattat cagcagggga
     1321 agctctagga aaacaaagtg cggaatttat attagagaca atagattatg accatatagc
     1381 tagaataaag aaagctatag aagaaaaagg ttttaaaatt atagaattat aataaaaggt
     1441 gtatataata tacaagaaaa atagtataat agtgtatgaa gatacatttt atatgtatta
     1501 gattaacgaa ttaggaggat tttaaaatga aacatgaagt aatacatact aatgatgcac
     1561 cagcagcttt aggaccatac tctcaagcta taaaagctgg aaacttatta tttgtatctg
     1621 gacaagttcc tcttgtacca gagactatgg aagtagttga aggagatgta caagctcaaa
     1681 ctgctcaatc tttaaaaaat cttaaagcta tattagctga aagtggagct gatttttcaa
     1741 atgtagttaa aactacagta tttataaaag atatgaatga atttggagct ataaacgaag
     1801 tttatgctga atattttgga gaaaataaac cagcaagagc ttgtgtagaa gttgctagat
     1861 taccaaaaga cgttaaagtt gaaatagaag taatagctgt attataaaaa tacaatattt
     1921 ttaatttgaa aaggctgaag aactatattc tagtgattca gcctttttat aaaaaataaa
     1981 tcaaaagctt aaagagcttg ataaatattg tcgaattaaa aaataaaatt aaaataaata
     2041 ttgcaatatt tttgagaagg ttttataatg agtatataat ttgaaacgtg taactattaa
     2101 attaggcatg agttcttgga tatttttatc ctgaggattc atgccttttt tgtattgaaa
     2161 atcaaaaatc tatttaataa ggaggaagta ctgtgttaaa ttttttcaag aaaaataaaa
     2221 gctataagtt acatgcagta gtgagtggta actgtattaa tattgaaaaa gtaaacgatt
     2281 ctgtgttttc aaaaaggtta atgggagatg gagttgcaat tattcctaat agtaatgttg
     2341 ttgtagctcc ttgtaatggt aaagtaactg tgcttacaga gtcaaagcat gcatttggta
     2401 tggtctcaga tgagggtgtt gaaatattag tccacatagg aattgataca gttaatttac
     2461 aaggtgatgg gtttaaaaat gaagtgtctc aaggagataa tgttaaaaaa ggtagtccaa
     2521 taataagctt tgaaagagaa aaaataaatt ctcagggaat tgattgtaca ataataatgg
     2581 ttgtattaaa tcatagcgaa ttttcagaaa taaattgcat ggtagaaaat gaagttgttg
     2641 cagggcaaga tactgttatt gaaattatga aatgatagga agggattaat tcaatatgag
     2701 agtagtaaaa atctttaata ataatgcatt atctacagta actaatgata ggaaagaagc
     2761 tattttacta ggtcttggta taggttttaa taaacgtcca ggagataaag ctaatgaaga
     2821 taaaatagaa aagatttatt atgttcaaga ccacatgcaa acaaaatttt tagaattgtt
     2881 aaaaaatgtt agtccagaag tgatggatgc ttcacaacaa attataagtc ttataccagg
     2941 gaatgaggga aaatttaata ataaagggat attatcttta gtggaacata ttagttttgc
     3001 aatagataga atgaaaaata atgtttttct acctaattta atgcttttgg atataaagat
     3061 gatgtattca aaagagtttg agttaggagt aaaagcttta gaagttatat acagagtatg
     3121 tcatatccat ttgcctgaag atgaagcagg ttacatagct ttacattttg tcaatttaca
     3181 atcaaatgat aatttggcat atgatacttt aaaatttgta aaaggtagta ttaatttgat
     3241 taaagaatgt tatggattag aattagacga atcgagtttg agtacattac gttttaggac
     3301 tcatcttaaa tttcttgctc aaagaatttt taagaatgaa atttgtcagg atgataaaat
     3361 gattgaaatg tatgattatt taattaacaa tcatcctaaa aataaagaat atttagaaaa
     3421 gttaaatata tatattgaaa aggagtttag gtataaatta gataaacctg aaaaaattta
     3481 tctactgcta cacttaacaa aaatcttata agaatgagat gaattagtaa tagaaatata
     3541 tataatttta aggggggata agattgaaag ataaactact tggaggactg gaaacatttg
     3601 caaaggcaat ggttcagcca ttaatgtatt tatcaagtgc agggatatta atggtggtag
     3661 gtgtactact tacaaatgat atgttactag gagtatttcc attttttaaa tgggagccaa
     3721 ttcaaatatt tggaagccta atttataact gtataatggc tattattaat aatcttgctg
     3781 ttatatttgc tataggtatt cctgctgcac tagcaaaaaa agagaaacac aaagcagcat
     3841 taataggatt tatgagttat ataatatacc ttacagcttc taacactatg ttaacatcac
     3901 taggtcaaat agcaaaagaa agcaagatgc ttgggttgat agggactgga caagctacta
     3961 ttttaggtat tcaagttgta gatacaagtg tatttggtgg aattatttta gggtgtatag
     4021 ctggatatgt atttaatcgt acaagtaaca aaacatttaa aggagcttta cagatatact
     4081 caggagcaaa ttttagtttt atatgtatgt tctttgtatc cattttaatt ggcgttgtga
     4141 gtaactttat atggccatta gctcaacaag gaatagcttc tcttgcaaat gtcatcaaag
     4201 atacaggtac atttggttta ttcttatatg gttttttaga gagaatatta gtaccaactg
     4261 gacttcatca tttaatctac acaccatttc agttttctga tttaggagga gtgcttaaag
     4321 ttggtgtaga tacagtttca ggagcttatg ctatagttat gacagaaatg aatttaccag
     4381 ttgagcgttt ctcagatagt atatactaca tggcaactgg atttacaaag atgtttggtt
     4441 atataggtat aggagctgcc tttatttata ctgcatataa gcaaaataag aagaaggtta
     4501 aggcaacgat tattccacta ataattacgg cagtattagc ctctgtaaca gaaccattag
     4561 actttatgtt tgtattctgt gcaccacttt tattctttgt acatgcagtt atagctggtc
     4621 tatttatagc tttattaaaa atatttgatg ttactgcctt atgtggaggg aacttattag
     4681 tatcaattat aatgaatact acagtaggtg tagaaaaaac acactggcca gtaatgatga
     4741 tattaggact tgcacaaatt attgtatatt ttgtagtatt tagtttttta attaagaaat
     4801 ttaattataa aactccaggt cgtgaagatg agagtacagc tacaggcgaa ggacaatcaa
     4861 aacaacttaa tttagataca ggtatagaaa atataattga tgggttaggt ggaaaggaaa
     4921 atattaatac agtagaaaat tgcatcacta gattacgtgt taatgtaaaa gatgaaagta
     4981 agattaatga agatataatt aatttaacgc caaatagcgg gattgtaagg aaaggaaaag
     5041 atatacaaat aatttttgga cttcatgtgc atgaggtaag acgtgcagta gaagattttt
     5101 tagaaattta ttaaagatac atataattta tatagagttt atagaggagg aataattatt
     5161 atggttatta ctattgttgg aggaggaagc acatttactc caggtattgt taagtcaatt
     5221 gcattaagag aaaaagagtt agagcttgaa gaaattcgtt tatttgatat tgataaagaa
     5281 cgtcaagata aagtagctgt agttgtcaac tggatactaa aagaagatgt taaaacagat
     5341 gttaaactaa ctgtaactaa ttgtgaaaaa gaagcttata cagatgctac atttatattt
     5401 gcacaaatgc gtgttggtaa atatgaaatg cgtgagcaag atgaaaaaat tccattgaaa
     5461 tatggttgtg taggtcaaga aacttgtggt tgtggaggga tggcatacgg attgcgtaca
     5521 atacaaccaa tgataaaggt tattgatgat gtagagaaat atgctaaaaa aggttattgg
     5581 atattaaatt actctaatcc tgctgcaatc gtttctgaag cttgtcgtaa gttaagacca
     5641 aatgcaagga ttataaatat ctgtgatatg ccaatagcta ttatagatat gatagcgggg
     5701 tcattaaata tcaatgacgt ccataatata cgctatgatt attttggtct aaatcatttt
     5761 ggctggttta cttcaataga ttataaacat agagacttaa tggaagagat taaggaatac
     5821 ataaaagaaa ataaaatatt actaccaaaa tcttatatag aaaacttaaa taaaagtcgt
     5881 agtacaggaa atcgtcatgc tgtaagtagc tggatacatg tttgggaatc tgtttataca
     5941 atgttagaaa acttcccaaa atatctacca aacacttatt taacatacta tcttttaagt
     6001 aaagaaacag tggaacattc aaacataaat cgtactcgtg caaatgaggt tatggaaagc
     6061 cgtgaaaaag acttatttga aggtgtgaga cattacttag aaactggaga aatttctgaa
     6121 aaagcatttt atgcaggctc acatggagat tggattagtg atttggcagt ttcaattaaa
     6181 aatgatacac gtagtcgttt tttagttata actgaaaatc gtggagctat cccaaacatg
     6241 ccatatgatg caatggtaga aattccagca tatattggta aagatggtcc agaagttatt
     6301 gctcgaggtc caatacctct attccaacaa ggattaatga tgcaacaact taacagtgaa
     6361 aaattgatag tagaagctta tatagaaaat agttatgaaa aagctttaca agcattcaca
     6421 ttaaataaaa ctattccaag tatgaatgtt gctaaagaaa ttttggatga aatgatagaa
     6481 gttaataagg aatactggcc agagctaaga tagtaggata gaagaaaagg tttttattta
     6541 ataattaggt atgttttcag agtcttttat caaaatgtat tcacaaacat atggcaatat
     6601 attaaagtaa agagtgatat ttttagaaga atggggtgat gcaatgaaca ttataaataa
     6661 aattttttgg caagaagata ggattggcat gattacaaat aatttagagg cagatacaca
     6721 tagtcactgc atgttacaaa tgttcttagg gatagaagaa aacattgaaa taatagtcaa
     6781 tgaaaaatta gttaaatgta attgtatcgt agtagataaa aatatctctc actcattttc
     6841 agcaagaaaa aaagtttatt actctgctat tattgaacct acatctatat atgcagaaca
     6901 gcttacctca aaaatgagtg attttggcta ctggatatgt gacaatgatg gattagagaa
     6961 attacgtcaa caaggaactt ttttgatata taattctagt aaagaacaat atttaagatt
     7021 tatggaaatg cttaataact atttgaatat acctataact ctcaaaaatt atgatgacag
     7081 aattactgaa ttgctcaatt tattacgtac ttgcaattgt gataatcata ctatttcaag
     7141 ttttgcagat aaagtgtccc tatcagcaag taggctatca catctgttta aataacaaat
     7201 agggatacct ttaaaaagct atatcttatt tcatcaaatg gagtgtgctt ttagagaatt
     7261 actttctgga aaaaatgtta ccgaaacttc tatgactgct ggattcgata ctccatcaca
     7321 ctttgcaggg acagttaagc gaatgatggg aatgcccgtt tctttatctt tgaaagatag
     7381 cgagtttttg aaagtatatt gaatattctc gttatataat gaagttgtat taagaaaggg
     7441 gctttattat gaataaagaa tatttagaag aaacaaaaat gttaaattat aatgaaccac
     7501 aacttaagct cttggtatca tcaaagaagt ggttggaatt agatgatttt tataaaataa
     7561 aatctattta tgagtttgta cagaatgata ttttatttgg atataatgct tctgatatat
     7621 taagcgcaac acaggtatta aatgatggta tgggacaatg caatacaaaa gctactcttt
     7681 taatggcatt attacgtgca gtgaatatcc tttgtaggct tcatgctttt gatgtaacaa
     7741 aagattttca acgaggtgct acatccaaat tgatttcact attagcacca aaatatattc
     7801 ttcatacttg ggtagaggta ttttatcaag ataggtggat tgctctggag ggtgtcataa
     7861 ctgataaaaa atatttagaa gcaattcaga agaaattttc taattatggt ggaacgttta
     7921 aaaagtatgc aatagctaca aatgatttga aaaatacatc tattgattgg gatggtaaag
     7981 atacttttat ccaaaaagaa gctatcgtgt atgattatgg tatttttcca tcaccagatg
     8041 tatttttttc tactcattca caacatatgt caaaattaaa gaatttcata tatgtacatc
     8101 ttatcagaaa aataatgaca aaaaatgtat gtaaggctag aaataattat attgataaaa
     8161 acgaatgagt gtttaagaag tgactatata ggaaatatat agaacaatta aaactgtatt
     8221 ggttaaaatg aataaaaagg agttatcaaa gagcaaactt aaattgttca taggataact
     8281 ccttttttat attaacttct aataatttgg tgtcttttta taaaacatat agatacaatt
     8341 aaaccaacaa atgcaagagt agctgctatt gaaaatgcaa catctattcc atggatatta
     8401 gacagtatag cagaactcat gtgagctttt ttagtagcac ttgtcattat agtaataagc
     8461 acagcagaac ctatagcacc agatatctga cgaagtgtac tattaatcgc tgtggcatga
     8521 gatatgagtt ctctgtctag tgttttaatt ccccatgtag ttaatggcat caaaaccatt
     8581 gagataccaa tcatacgaat acaatacatc aatgatacat gaagtaaaga tgtatcccta
     8641 cctaaaaaag caaaagatag agttcctaaa aataaacatc cagttcctaa tatcgataga
     8701 gcatgaggac catatttatc taaatgacgt cctgcaactg gatttaatac aaccattaat
     8761 atagcgccag gtaacattaa tgaacccgaa gacaatgcag aaaatcctct tacagactga
     8821 acatatagag gaatcattaa tgtagcagac atcattgaag catatacaat cataattaat
     8881 attgtagaaa tgagaaatgg tttattttta aaaaccctca attctaaaaa tggttctcta
     8941 gattttaatt gacgtagtgt aaagaaaatt aatgatataa ttcctatgaa tattggtaaa
     9001 tatgtggcaa tattagtcca cccataatta ccttgattag taaatccaat taacaaacca
     9061 ccaaaaccta gagatgacag tattattgaa ggaatatcta attttagttt ttgcgtttct
     9121 ccaacatttt ttagtagaat aaatgaaagt ataacatcta gtatagctat aggagaaaga
     9181 agcaaaaata aactatgcca gccaaatgaa tctactaacc atcctgataa agttggtcct
     9241 attgctggag caaatcctac tgtaatacca actaaactca tagctgcacc acgtttttcc
     9301 tcagggcata aatatagtat aataacttgt aaaagtggca ttagagaacc agaacccatt
     9361 gcctgcaaca cacgagcaac caacatagtt gaaaaattat tagaaaaaag tgcaataata
     9421 catcctatag aaaatataga cattgatgta atgaataact ttctagtact aaatctattt
     9481 atcaaatagg ctgttgtggg cacaataata cctgcaataa gtagatatat tgtcgttaac
     9541 cactgaccaa catcagcagt aattttaaaa tctgacatta tattaggtaa tgctgtagat
     9601 aatagtgttt gactaagaga acttataaaa gcaccagtca ttacgattgc tattattaaa
     9661 tttccttttt tattgttact ttgggtttcc ataatttaca acgtctcctt ttttaattga
     9721 tttttaatgc tgttcaataa aagtaccttt ggtctcaaag tgtcttcttt ttccaaatat
     9781 gcaatcattg aatccataat taaagaatca tattgttgat tttttccctc gacatataat
     9841 ttttttgttg cttctagtaa tttagaattt tcaaggagag aagtatctgt tttgattagt
     9901 tcttctatat cttgtatcgt tttaaaaaat agcgatttat tataaaggga aaagtaagct
     9961 tcatcttctt gtttatgtaa aaacattgat ttttcaatta aaggatttag attatccata
    10021 ttttctacaa ctaaatctag aatttgtata gtaaatttag caacaaagta tacatttatt
    10081 tctcttttct gtacatgaat aatgtatata aactgatgaa ttagagcatc caataagaaa
    10141 actaaatcaa atatgatgtt ttctatttta tcaccatagt tatcaaataa gcttagcttg
    10201 tattcattca ataagttagc tcttgatttt gtttgcaatt tacttgtagt cttacgttgc
    10261 acttcagaaa aattttctct tatgtaggta tttatggtag ttctagaaaa aatattttcc
    10321 catacagcaa ttatctttct gtttaacttt tcagatgatg gaatatcatt attttcattg
    10381 atagaatgta taatttctaa aaacttttca tcataataat tgattatttc gcagataaaa
    10441 ctttccttag tttcaaaatg cttgtaaaaa gtagctttag acattttgca ctcttttaca
    10501 atgtccgaga ttgaagtatt ttgaaaacct ttttggtcaa aaattttttg tgcaatagtt
    10561 ataattagcc tttgtttttc tgtcacttta ttaccttctt tcatataaaa aacgtttagt
    10621 actatatagt acctataagt tttaataaat atattataca tcatttggaa aaaaaatcaa
    10681 tatatacact gtaatatgaa atttttgaaa aatttattgt tatttttagt cgtataaaaa
    10741 agaaacttaa gagtttctta agaattgttt aaataaattg caataaatta gtggtatgat
    10801 tatagtatga aagagaatgt cttgaaattt ttctgtatca aattccaatt atggaggtgt
    10861 agtatatgaa aaagaaaatt tgtgtagcca taatttttgc tctatttata gtttttgtag
    10921 aaatgtttat tgatagttct agtgaaggaa gtaaaattat gacagctgaa ttaagtaaat
    10981 acaggaaggg cgtaaatgta ttatcaataa acaatattat agtagcgaat aaaaaatata
    11041 gtttacctaa agactattct cctcaggaaa gtagtgaagc gagaaatgct ttttataaaa
    11101 tgaataaaga tgctcaaaaa tcaggattaa atttaaaagc ttttagtaca tatagaagtt
    11161 atgaatacca agatagatta tttaagtcat atgtaaaaga acatggagaa aaagaagcaa
    11221 atagattttc agctaaacct ggagaaagtg agcatcaaac tggacttgct tttgatattg
    11281 gggggaatga ccagtcttgt tgggcaaata aaaagtttaa caatacaaaa gaagctaaat
    11341 ggttgtatga aaatgcatat aaatatggtt ttatacttag atatccagaa ggcaaagagc
    11401 atataacagg atatatgtat gaatcttggc actatagata tgttggaact gaacatagca
    11461 aaaactttgc aatgaataac ttgactctag aagaatattt acatataaat taagagatag
    11521 tttgttaaaa ttaggttaaa ataaaaaata aactagacta ttaattgtgt aaagtgatat
    11581 aatgtaaata taatttaaga taactactcg aaggggagta gcttacacga aagtggtaat
    11641 taatcgtcaa gacagtagaa atactcggtt aattagcaag ggattccttg atagcaagac
    11701 cttcaaaata agcattaggt atgtttgttt tggggtcttt ttttattcaa ggaattttta
    11761 tttaaaaaaa tttaaatttc aagtatacat aagagccccc aaacataaaa taaatttatt
    11821 gaagaggagg gcaacaaatg tcttacaaaa gtacttatca agaagtaatt aaaaatttag
    11881 actcaagttt atctggtttg agtaaagaaa gagcagaaga gttgttagaa aaaaatggag
    11941 ctaatgaatt aaaagaagca gataaggttc ctacttataa gcttttccta gaaagtttta
    12001 aagacccatt agtaataatt ctattgatag ctgctttagt acaaatattt ttaggtgaaa
    12061 cagtggagtc tataataatt tttgcagtta ttattataaa ctctgttctg ggtgttgtac
    12121 aaacaaaaaa agcagaaagt tcattagaaa gtttgaaaaa tttatctgca ccaaacgcaa
    12181 aggtaataag agacaataaa aaaatgacaa ttccagcaaa aaatctagta gttggagata
    12241 ttgtattctt agaagctggc gattacatac cagcagatgg aagacttata gaagcacaaa
    12301 ctctcaaggt tgtagaaggt atgctaactg gagaatcaga accagtttta aagcatacag
    12361 aaaaaataag tgaagatgta agtttaggtg accagaaaaa tatggtattt agtggctctg
    12421 tagttgttta tggtagaggt tcttttatag tgaccggaac aggtatggat acagaaatgg
    12481 gtaaaatagc tgggctttta gaaagtgctg aaaataaact tacaccatta caacaaaaaa
    12541 tagaggattt tagtaaaaaa cttggtgtag gcattgtaat acttgctctt ttaatattta
    12601 taattcaagt agcaagaaat tactttatgg ttggaaatgg agaaatgaca caaattattt
    12661 tagattcatt catgttctct gtagcagtag cagtggcagc tataccagaa gctttatcgt
    12721 caatagtcac tatagtattg gctgttggaa caaatagtat ggcaaaaaaa caagccataa
    12781 taagaaaatt accagcagta gaaactttag gttcaacaag tgtaatatgt actgataaaa
    12841 caggtactct tacacaaaat aaaatgacag tagttgatta ttatatgtat ggaactaata
    12901 atgatgagtt agataaagaa aaagtaaact attcttatca tgctaaatta ttgactatgg
    12961 tttctacatt gtgtaatgac tctcatataa ctagtgatgg taaagaagtt ggcgacccaa
    13021 cagaagtagc tttaataaat tactcaagta aaaatgattt agattatgat aaattaagaa
    13081 gtaagtatat tagaataaat gaaataccat ttgactcaga tagaaagctt atgtctactg
    13141 taaatagtat atatggagac tatataatgt ttacaaaagg tgctccagat attgtgttta
    13201 gtagatgtaa atatgctttg aaaaatggtt caaaagtaga tataacagat gagataatag
    13261 aagaatataa acataaaaat gaagagtttt caaatagagc acttagagta ttagcttttg
    13321 caataaaaga tgttccagaa gatgattttg taccttcaat tgatgatgaa catgacatga
    13381 cattggttgg aataatggca atgatagacc caccaagaga agaagtaatg gatgcagtaa
    13441 aagaggcaaa gagtgctggt ataaaaactg ttatgataac tggtgaccat aaaacaacag
    13501 cagcagcaat tgcaaaagag attggtataa tggaagatgg agacctagct cttactggaa
    13561 aagagcttga tgctttgagt gaagaagagc tatatgaaaa actagaaaat ataagtgtat
    13621 atgccagagt ctcaccagaa aataaaattc gtatagtaaa agcatggcaa cataaaaata
    13681 atataacagc aatgactggt gatggtgtta atgatgcacc tgccttgaaa caagctgata
    13741 taggaatcgg tatgggaagt ggtactgatg tagcaaaaga tgcttctgcc atggtattgg
    13801 ttgatgataa ctttgcaagt atagtaaatg ctgttgaagt tggtagaaca gtatacagta
    13861 atataataaa atcaattact tatctatttg caggaaactt aggagctata gtatccatat
    13921 tatttgcagt atttgctggt tggtcaaatc catttacagc tttacaactt ttatttataa
    13981 acttagtaaa tgactcttta ccagcaattg cattagggtt tgaaaagtca gaaaaaaata
    14041 taatgaataa acctcctaga catccggatg aaagtatact agcaggagga acattgaggg
    14101 cagttgctat aagaggttgt ataataggta tagttacaat aattgcacaa tatataggat
    14161 tgcaagtatc tccagaactt ggagtagcaa tggcattttc aacattgata ttagctagaa
    14221 taatgcaaac attacctgca cgttctaata atcaaacagt aataaagtta ggttttttta
    14281 gtaataaata cgttatagga gcagttctag tctgtcttgg gctttattct ttaacactaa
    14341 taccagtgct aagaggaata ttctctatac caacaacatt tggattgtat cagttgggaa
    14401 tttgcttagg tttagcattg atttcatcaa tattaatgga acttaacaaa ttaataaaca
    14461 gaagtgaagt aaaataaata aaaaattaaa gggcatattg attttaacaa tatgcctttt
    14521 ataaatagtc taacttattc tatattatca attacttgga atgcctttat aacttcaggt
    14581 aattcttttt cttcaggcat tctaaatctt atgaaatttt tatcaagtcc agtaaaatca
    14641 cttccagata taactcttat ttttaattta gcaaactctt gttgcaaatc tatgtccttg
    14701 ttcttatgtt caactgtcat aattgataca gtatcactag tttcagctat attaaggttt
    14761 ttccatggaa ttaataattg attttttata tcttttgttt tttcttttaa ttcttctata
    14821 aattggacat catctaataa attggctgca atactacgag atatttcaga tacttcgtaa
    14881 ggagtagaaa tctttttaat agggttaaca agttgttctg gaagaactgc atatcctgct
    14941 cttagtcctg ctaaaccaaa accttttgaa aatgttttta aagcaattac attatcataa
    15001 ttatttaaaa gcttaacagc tgaattttct tttggcatgt attcaccata agcttcatct
    15061 accataactg ctatatcata tttagctgct tcctttacta tattttcaat tgatgataaa
    15121 ggtataattt gtcctgtagg attatttgga ttgtctatat aaattacttt atattctgga
    15181 tttatttttt caataaattc tttttcatta aacttgaaat tatcctcttt ctttaaaaga
    15241 acatagtcat aagtagcacc atgcattttt atatctgttt catattctga aaactgaggt
    15301 gaatatccta aaactttatc tcccttttca ataaataatc tatttaaaag gtaaataaca
    15361 tgtatagaac catctgccaa acaaattctg tcagtgtcta atgttaagaa gtctttccaa
    15421 taatcgacta tattgtcttt taaagcaatt gaatgaggat atcctcttat catatcaaat
    15481 tttaaagcat taaaagcttc tacagctttg tttgagtaag atactgtatt tataccttca
    15541 ccacagtcta tctcgaatac atcttctgaa tgtccagatt ctattgcata actttttttc
    15601 aactgcattt caatagcttt gttaattttt aatttcatag tttatcactc cttatttagt
    15661 caataataaa tgttataaaa gttgctagat ttaaaatatt atgaaaatat ataatagtaa
    15721 aaaatgaata taaatattat aatagctcac tttatttaat atgattatat atcatatatt
    15781 gtaaaatttc tagcattata agactataaa ttgaaaatat tcagaaaaat actacaagaa
    15841 acgatttatt ttgctcttaa aataagttaa atgaatgtta ttaattttgt aaattgaaat
    15901 agtttaagta taaagttatg atataatcta ataagggtaa ttctttagat tatattaagg
    15961 aaatataagt atacaataaa aagttatgga gttggattat gggatataaa ttaattgtaa
    16021 cagatatgga tggaacttta ttaggaaata atcataaagt tacagatgaa aataaaactg
    16081 cactacaaaa agttataaaa aacggtatta atgtaacttt agcaacagga agagccttcg
    16141 attcagctaa atgcaatgta gattttctta aagaggacat gccaataata gcttgtaatg
    16201 gttcattgat aagagagcaa aacggaaaca taatatattc aaataagatt gatacaacaa
    16261 cttgtttaaa tatcttagat gtattagata aatacgatat ttattatcaa tgtaatagta
    16321 tagattctat gctttcaaaa aaaatagaag gacgagaaga tagattaagt gtatttttag
    16381 gctctgagac agaagtaatt ataagagatg atttgagaga agaaattttt aaaaaagata
    16441 tactaaaatt tgtaatcata gaagaaaaga atccatctat tttggatgaa ataagaaaag
    16501 aattgagaaa agttcaagga ataaagataa cctcttcatg gccaaacaat attgaagtca
    16561 tgaatgaagg tgttgataaa ggtaatgcag ttaaaatttt agctgaaaaa atgaatatag
    16621 atagagaaga tataattgca tttggagata attacaatga catagaaatg ataaaatttg
    16681 ctggtcttgg agtagctatg ggaaatgcag aagaattgat taaacaagaa gctgattatg
    16741 ttacagatac aaatcaagac agtggagttg caaaggcaat ttataaattc ctagagttaa
    16801 aagaatcact tagttcttaa aatgatatat aattaggaaa aaataggttg actattttgt
    16861 agatagtaat tataatacaa atagataaaa cataaaaatt tagattcagt gaaaaagaga
    16921 gtagatatgg agaaaatctc agcgaattgg ggatggtgtg agcctaatga taacacttta
    16981 tattgaaaag aactttggag aatactactt gaaattcagt atggtagtag gtaatctcgc
    17041 cttaagagaa tagagtgggt aaacattgtt tgccaactag agtggtaaca cggaatattt
    17101 cgtctcttgg tctttttgac caagagattt tttagtttca ttaagctata agaattataa
    17161 ttaaaataca aaaaaataaa atatgaggtg aaagttatga aattatgggg cggacgcttt
    17221 agaaaagcag aaaatcaact tatggaagaa tttaataaat cttttggcta tgattgtata
    17281 ctgtacaaaa aagatatcga aggaagtgta gcacatgttc atatgcaagt aaaatgtgga
    17341 cttttaacag aagaagaagg aaagtctata acagaaggat taaaagggat acttgaagat
    17401 gtagaaaatg gaaagttagc attagatgga gagtatgaag acatacatag ttttactgag
    17461 ataaatttaa ttcaaagaat aggagatgtt ggaaagaagc ttcatacagc acgtagtaga
    17521 aatgaccaag ttgcagttga tatgagattg tatgccaaag aaaaagcaaa tgatttggta
    17581 gatttaatat ctgaatttaa agcgactata aaagatgttg ctgataaaaa cccggtgatg
    17641 atgccaggtt atactcatct tcaaagagca caagtagtga catttaagca tcacttaatg
    17701 gcatattaca gtatgtttga tagagatgag aaaagaataa aaaatgctat agaaatatta
    17761 gatgaatctc ctttaggttg tggggcttta gcaggaacta cacatgatat agacagaagt
    17821 ataacttgtg aacaattagg ctttaaaaaa gtagtagata attttatgga tggagtaagt
    17881 gatagagatt atttattaga actaatgagt gacttttcaa ttataatgat gcacttaagt
    17941 agattaagtg aagagttaat actttggagt tcacaagaat ttggatttgt tgagatagat
    18001 gatttatata caactggaag tagtataatg cctcagaaga aaaatccaga tggtgcagag
    18061 cttataagag gtaagactgg cagagtatat ggaaatttat ttggtctttt tacagtaatg
    18121 aaaggaatac cattagcata taataaagac atgcaagaag ataaagaagg tttcttcgat
    18181 agtgtacata ctcttgaaat gtgtatacaa ataatggata gaatgatagc tacactaaag
    18241 gtaaatgagg ataaaatgaa acaagcagta aagaatggtt ttttaaatgc aaccgaagtt
    18301 gcagattatt tagttaaaaa taatgttgct tttagagatg cacatggtat agtaggaagt
    18361 atcgttatat attgtgaaga taataaaaaa gcaatagaag atttaacatt agaagaatta
    18421 tataagtttt cagatgcttt taaggaagat atttatgatt ttatagacta tgagagtata
    18481 ttaaataagg gaataaaaaa gaatttaaaa taatatactt atttctaaaa gctgtagatt
    18541 tgaatgttat cattcaaatc tacagctttt tattg
//
LOCUS       DAMJVM010000068        18358 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.071, whole genome shotgun sequence.
ACCESSION   DAMJVM010000068 DAMJVM010000000
VERSION     DAMJVM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18358
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.071"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            164..1324
                     /locus_tag="PC523_002502"
     CDS             164..1324
                     /locus_tag="PC523_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HDF2476013.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGIYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGTGSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPRFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISRLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLS"
     gene            1664..1825
                     /locus_tag="PC523_002503"
     CDS             1664..1825
                     /locus_tag="PC523_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476014.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            2018..2647
                     /locus_tag="PC523_002504"
     CDS             2018..2647
                     /locus_tag="PC523_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDF2476015.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLNCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTFKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVVLCGDFNEKNVFLSMFNDMA
                     ISLNKSYLSTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            2887..4146
                     /locus_tag="PC523_002505"
     CDS             2887..4146
                     /locus_tag="PC523_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDF2476016.1"
                     /translation="MGRRATRVSRLGKEKKRNIVIVLVVLIIAMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKSEALRAITDKYTPEDLNLTYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLNYTDSVIGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLAGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            4315..5310
                     /locus_tag="PC523_002506"
     CDS             4315..5310
                     /locus_tag="PC523_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HDF2476017.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNL
                     EEKMLQSIDRGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVVQVKKDYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            5556..6623
                     /locus_tag="PC523_002507"
     CDS             5556..6623
                     /locus_tag="PC523_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDF2476018.1"
                     /translation="MSNIKIIASNKCWIEDIAKKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGILKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYAKDGLEVESEKAIDYIKNHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTKISLYSLSH
                     GAGRKWSRSVCKSRLKSKYNKDTIKQTKFKSQIICSDLNLLFQEAPEAYKNIEQIIES
                     LMEHKLIQVVATMKPLITYKG"
     gene            7137..9047
                     /locus_tag="PC523_002508"
     CDS             7137..9047
                     /locus_tag="PC523_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDF2476019.1"
                     /translation="MNSNTFERLQLNEVKELIKIHCVSSLGKNLIDKLNPSGNIGVVR
                     RKLAENKEARKIIENSNHVPLEGLFNAGSTIDKIEKGMVIEPIELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKNIEDEINYCIKSNKVDSNASKELKKVRRNIEIT
                     EAKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVDGTILDTSSKGNT
                     VFIEPISVSNLSTELIMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMNLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNVEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPIDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDTLFTNFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(9110..9733)
                     /gene="ytaF"
                     /locus_tag="PC523_002509"
     CDS             complement(9110..9733)
                     /gene="ytaF"
                     /locus_tag="PC523_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HDF2476020.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFFLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLFGCLLGNMFVKNINLNLSWLSGLILLVIGFIKIC"
     gene            9921..10565
                     /locus_tag="PC523_002510"
     CDS             9921..10565
                     /locus_tag="PC523_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2476021.1"
                     /translation="MEQHSKVGNFGDLLKILLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFKNNNNQIFNTDSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            10770..11828
                     /locus_tag="PC523_002511"
     CDS             10770..11828
                     /locus_tag="PC523_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HDF2476022.1"
                     /translation="MNCKWISKIDERKKCDREADSSGYCIFHKENKSDEEIQLMVDTL
                     NKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FKDKVLFNGCVFLENCDFNRTIFSKDYINDRIFEKVKFKGSDLIVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIVIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFRMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            11932..13116
                     /locus_tag="PC523_002512"
     CDS             11932..13116
                     /locus_tag="PC523_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2476023.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDNYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTSSGDLLGSVDIVSNNSIDNVPLKTKLKMKF
                     AN"
     gene            13323..14036
                     /gene="rpsB"
                     /locus_tag="PC523_002513"
     CDS             13323..14036
                     /gene="rpsB"
                     /locus_tag="PC523_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDF2476024.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            14115..15026
                     /locus_tag="PC523_002514"
     CDS             14115..15026
                     /locus_tag="PC523_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDF2476025.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            15110..15814
                     /locus_tag="PC523_002515"
     CDS             15110..15814
                     /locus_tag="PC523_002515"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDF2476026.1"
                     /translation="MDKPMYKRVLLKLSGEALAGEKGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            15840..16397
                     /gene="frr"
                     /locus_tag="PC523_002516"
     CDS             15840..16397
                     /gene="frr"
                     /locus_tag="PC523_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDF2476027.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEDFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            16400..16603
                     /locus_tag="PC523_002517"
     CDS             16400..16603
                     /locus_tag="PC523_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476028.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQDINYTIKSIEGKKDKDKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            16632..17366
                     /locus_tag="PC523_002518"
     CDS             16632..17366
                     /locus_tag="PC523_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDF2476029.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            17379..18158
                     /locus_tag="PC523_002519"
     CDS             17379..18158
                     /locus_tag="PC523_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDF2476030.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVNPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINEFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
     gene            18133..18306
                     /locus_tag="PC523_002520"
     CDS             18133..18306
                     /locus_tag="PC523_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA helicase"
                     /protein_id="HDF2476031.1"
                     /translation="MRLNFLLDKDWLDIKEQSIIASNENSYVTFKSRDILQLLVTWLF
                     SLFDNLENIKEIL"
BASE COUNT         7571 a         1840 c         3184 g         5763 t
ORIGIN      
        1 tatgcaaatc tttattaata tgtgtcaata gttaggtata tatgtattta attagcatga
       61 tatagataag agtatttatt ttaattgtct attatctgaa aaagtcttta cttaaaaatc
      121 ataaaaaatc aaattaatta ggaaaagaaa gggtgtgtat aatgtgattg agactggatt
      181 tgtatattgt agtttcttgg ttgtattcgt aggtgtaatt ttctttatgg ttgataaatt
      241 gaagtggaag gtatttgatg ttattaatcc aatgctttta atatatttag gttcagctat
      301 acttggatca tttggtttat ttgcacagaa tgaagatgtt ggaatatatc aaaatataat
      361 aacagtgaac ttccttccat taatgttagt gttcttgtta attcaatgtg atttaagaaa
      421 aatactaaag atgggaccta aaatgttatt atcatttttc tgtgctacat ttacatttat
      481 gcttgggttt gttatagcat tcataatttt taaaaattca atgggtacag gttcatggaa
      541 aattcttggg gcagcatctg gttcatggat aggtggctca tcaaatatga ttgccgcagc
      601 taatgctcta ggagttgcag aagaaggtct tagttatgca atacttatgg gttctatagg
      661 ctatacagta tggatgtctg tttgtctaat gtctgtgaaa tttggaccta gatttaacaa
      721 atggacaaag tgcgatacaa gttttataga tgaggttaca tcaaaacttg aaaaagaaaa
      781 taaagataat acagcaccta cgtttgtaga acttatgatt ttactttctg ttgggtttgg
      841 tgttgcttct tttgcacaaa ttgtatctaa gatacttcca acttcaggga tacttaatga
      901 aactatgtgg gtagtacttg ttgcatctac agtgtcaata ttattgggta tgacaaaaat
      961 atcaaggctt aaggggagca cattggtagg aaatgtgttt atgtatttac tccttgcagc
     1021 cacaggtagt aaggcaaatt tctttggatt gtcagaagca cctgtttaca taatgtttgg
     1081 attggtagta gtccttgtac atgctatttc atttataggg cttgctaaac tatttaaact
     1141 tgatttattt acttgtgagg taggaagtat agctaatatc ggaggagtaa ctacagctcc
     1201 tatgattgct ggggttcaca atcctgtatt aataccagta ggtgtactta tggggcttct
     1261 aggaaatatt ataggtactt attgtgctct tatggtaact caaatactac atatgttatc
     1321 ttaataaaaa tataatataa aattaatcat ttaaataaga aaaaacattt caatttaagc
     1381 ataagctttt attgaaatgt tttttatttg acaagataat ttatttcaag aagatatttt
     1441 gacatattta gcgtataata ttgtaaaatt tactaaaaat gagatatatt taatttttat
     1501 attgaatcga acgtctgtat gatgtataat aaatatatag aacgaatgtt tgcagaacgg
     1561 atgttttttg aatttatttt aataatttat gaatatatat cagaatgcat aaatatattt
     1621 aaatataaag attaaaaaga atttaaagga gtgttgataa cttatgataa ttccatcaag
     1681 aagaatacaa gatataacat taacaaattt aaaaaatggt gaagtaactt taattgaatt
     1741 ggatgaaata tacaagaaga tgggattttt atttgttgtt agtgaaggca aattaaaaaa
     1801 gataaaaaag gagaatcatc attagaaatg aatgaaataa taaatattat aatagtttat
     1861 aaaacctaac ttatgtagaa tataaatact taagttaggt ttttttgtga taaagtttat
     1921 aagtactcaa ttttataaga taggtaatag tcttaagtga aaagtaaata agtttaaatt
     1981 ttaataagta ttatattaca taagaggtga tattattatg aaaatagtaa cctacaatat
     2041 tcataaaggg atggattcaa ataatagatt aactctaact aaaatggggt tatatttaaa
     2101 acaattgaat tgtgatgtaa tttgtttaca agaagtattg tatcctcagt ttttagcttt
     2161 aaaagctgtt ttaaatatgg atggtgtctt tgccacaaat gtaaaaaaag taaatatgat
     2221 atatggtatt tgtacattta caacatttaa aatgttaaac agtaatcatt ttttcttaac
     2281 tagcaaaaaa gaacaacgag gagctctttg tataactata gatgcatatg gaagaattat
     2341 aaatgtaata aatactcact tgggacttga tagacaggag agagcaaaac aattagacga
     2401 aataatagac tataggaata gattagtggg gatagttgtc ttatgtggtg attttaatga
     2461 gaaaaatgtc tttttaagta tgttcaatga tatggctatt tcactaaaca agtcttactt
     2521 gtcaaccttt gaaaaatcaa actctagaat agattacatt tttgttaata aaaatactga
     2581 actaaaagga tatacagtag aaaaaattta cctgtcagac cattatcctg taataggtta
     2641 tatttgaatt tcaatataaa aaaattaatt attataagat aaacaatttt tttgttaaga
     2701 aataataaaa aaaatataat aaaaaaatat caaaaataaa actaattata agacataaat
     2761 ttacaataaa gttacatttg attaaattcc ttgtttaaca aaagctattc aaatataatc
     2821 taaggtagtt ttgaaatggg aactctatta tgtaaaatta agttagtgtt aaaatgaggt
     2881 gaatttatgg gcagaagagc tacaagagtt tcaagattag gtaaagaaaa aaaacgaaat
     2941 attgtaattg tattggtagt tttaatcata gcaatgggtg gatttactta tttttttaac
     3001 agtaaatttc tgtacaatgg gaaaatcgct aaaaatgtat atatagaagg agtaaatgta
     3061 tctgatatga ctaaatcaga agcattaaga gctataacag ataagtatac tccagaagat
     3121 ttaaatctta cttatgatgg gaaaaaatat acaatatcac caaaagatat tgatttaaaa
     3181 tatgatacag aagatgttgt aaaagatgct tatgaaagta caaaaaaagg gtcctatttt
     3241 caaaacttaa aaaaatatat tgatataaga gtgaataagg caaatatgaa gataaaagct
     3301 gaatatgatg aggcaaagct tagttcgaag gtgtcttcta tagcagatag tataaatgtt
     3361 aagatgaaaa atgcttctat tagcgttgga agtggtggac taaattatac agactctgtt
     3421 ataggtagag aatttgattt ggcagcaaat aaagagtcta tatataatat gataaaaaat
     3481 aaagagcata aaactttaga acttaaagta aatcttcaaa aaccagatat aacaactgag
     3541 caagtaaaaa ctgtaaattc tgttataggt caatattcaa ccacttattc acaagctgta
     3601 gagggtaggt catataatgt aggattatca gcaagaaaaa ctagtgatgt attactaatg
     3661 ccaggtgaag aattttcata caataaactt acaggaccta gtaataaagc aaatggttac
     3721 aaagatgcac ctgttatagt ttatggtaag ctagaacaat cagcaggagg aggtgtttgt
     3781 caaacatctt caacagttta taatgctgct cttctcgcag gtatggagat aactcaagtt
     3841 acaaatcatt catctgcatc cacttatgta cctaaaggta gagatgctac tgttagtgat
     3901 gggggattaa acttaaagtt taaaaatcca tataagcatc ctgtatatat taaaaattat
     3961 gcaggtggag gaagtgtatc atcagtaatc tatggtaatt ctggtgacaa accgaatata
     4021 tctatagagg taaaacaaac tggtcagaat aaatattcta catatcgaat atttaaagat
     4081 tcaagtggaa aggtcataaa aaaagaacat atatcaaata gttcttataa agaattaaaa
     4141 aaataaataa ttttttagaa aaatatttgt aatataaaaa caaggaagtt tataacttcc
     4201 ttgttttttt gtaaaaatca acactattag ttcttgtttt aatattacaa tcattaatat
     4261 atagaaaata ttcctatata ttggtataat tgtaaataga tttagttata taaaatgtat
     4321 gaggaggtga catggttgaa ttttataggc aatagatatg aggtagtaaa ttctgatgac
     4381 gttttagaaa ttaacaaaat ctataaagct agagatgttt tttacagaaa aaatgttcta
     4441 ataaaagtta taaagcacaa taattatatc tgtgaagatt ttgtttcaaa tttaattgat
     4501 gaaagtactg ctttagatga gataaactct ccttatatac taaaaataat agatgttggg
     4561 attcattgta cagaagaaac aacattgtat tatgttgtaa gtgaagactt taatggtatc
     4621 ggactagatg aattaattct aggtaattat cttcatttag aagcaattgt aaacataatg
     4681 atacaagttt taaaagcttt agaaatgata cataggaatc attcatatca tggtagttta
     4741 aaatcaagta gtataatagt agatacagaa tacaatataa aaatttgtga ttttggaatt
     4801 acaaaagcaa ataatggggt taatacaaga agctatggaa acaggagata tttatgcccg
     4861 catcaactat gtataaatta taccgataaa gaaagtgatt tttttgcaac aggtcttatt
     4921 ctatttgaat ctatatttaa aaaattgcca tttggagaat caaatttaga agaaaaaatg
     4981 ttacaatcta ttgatagggg attagactgg aatagtataa aagcaattaa tggaaataca
     5041 gaacttataa atgttataaa aaggcttctt ggaagaaata acaagtatac tagagccaat
     5101 gatattatta ttgatttaag taaagtaatg tatgaaaaag catatataga agttgaagat
     5161 gagaaagaag aagaacagaa acaagataaa gttgtacagg ttaaaaaaga ctataaaagt
     5221 aaaatgagaa aaaaattaca taaaaaactt gttgttgctg gtctagctat agtcatgatt
     5281 tcaatgatta ttatcagttc cataatataa aaattgtata ttaagcattt taaaatcaat
     5341 atctataaat gatatacata gaaattttag tgtagtctta aattatgaaa ggaagtggtt
     5401 attatgttaa caaaaaataa tgaggtatta aatgtttagg agtattttaa caaatagcca
     5461 cctgctttta cagtttagaa taaaaaagct gtggctaaag gtcacagctt tttgctactt
     5521 tttaaaggca ggagcatatt aaaaaggagc aaattatgag taatataaaa ataatagcaa
     5581 gcaataaatg ttggattgaa gatatagcaa agaaacaact tgaagatatt tcaaagttaa
     5641 atggaatttt aagagtagta ggattacctg acttacatgc aggtaaaata cctgtaggtt
     5701 tagcagttga gactaaaaat attatttatc cacatataat tggaaatgat ataggatgtg
     5761 gaatgacttt atttaaaact gggattctaa aaaagaaatt taaaaaagat aaatggatta
     5821 agtcacttag caagattaaa gatttgtctg atatagaaat aaaaaacaca tataaagaag
     5881 aatgcccaat tttaaattta ggaactatag ggagtggaaa tcattttgta gaaattcaat
     5941 gtataagtga aatttataat aaagaacaat ttgaacaatt aaaattttca tctgatgata
     6001 ttatgatgtt agttcactgt gggtcaagaa attatggaga agatatttta aaaaaatttt
     6061 atgctaaaga cggacttgaa gttgagagtg agaaagcgat agattatatt aaaaaccatg
     6121 ataatgcttt aatgtgggca gagcgtaatc gtgaagctat cagtaaaaaa attatgcagt
     6181 caataggtac gtctggagat gttgaaacaa tattctctat aaatcataac tttatagaga
     6241 aaagggaaga taaatttata catagaaaag gtgctgtttc aagtgaaaga ggagctgtga
     6301 ttataccagg ctcaagaggt agtctatcat atatagttat gcctactgaa aatacaaaga
     6361 tatctttata ttctctatca catggagcag gtagaaaatg gtctcgctca gtttgtaaat
     6421 ctcgccttaa aagtaagtat aataaagata ctataaaaca aactaaattt aaaagtcaga
     6481 ttatatgtag tgatttaaat ttattatttc aagaagctcc agaggcatat aagaatatag
     6541 aacaaattat tgaaagcttg atggagcaca aacttattca agtggtggca actatgaagc
     6601 cattgattac ttataaagga taattttgaa agcaaaaaaa ttaaaataat attatagcta
     6661 gtcaataata aaacctataa aaatgtttta ttattggcta ttttttcttt gtagaaatga
     6721 taattttgag agttaaattc tctaacatat atatggttac aaagtaaggc tatattaaaa
     6781 attgaatata catgaattga tgccagcata aatgatttca aaatataaat taaataataa
     6841 atctattttt aatatgtaaa aatgtcaaat aaattatttt tattgacatg aatatctagt
     6901 ttatgaaata atattaggag cacttatata agtgtttaaa taagagtaga aaaggagcag
     6961 tgttttatga aaaaaaatac taacattaat gagtccggag ttaaacttct taataaataa
     7021 ggagtttgat gtatagtaac tccttattta ttaagtcgtt tacaaaataa tcattatcaa
     7081 aataaatggt atggtgattt aaaaaccaat gacttataaa taaaggagaa aaaataatga
     7141 atagtaatac atttgaaaga ttacaactaa atgaagttaa ggaattaata aaaatacatt
     7201 gtgtaagttc gcttgggaaa aatttaatag ataaattaaa tcctagtggc aatattggag
     7261 tagtaagaag aaagttagca gaaaataaag aggctagaaa gataattgaa aatagcaatc
     7321 atgtaccact tgaaggattg tttaatgcag gttctactat agataaaatt gaaaaaggaa
     7381 tggtaataga acctattgaa cttgtaaaca ttgaagattt tttaagaggt tgtaggaaga
     7441 tgaaagcttt tatgttagaa aaagaatttt attcacctac attaagttct tatgctttaa
     7501 atataacaga atgtaaaaat atagaagatg agataaatta ctgtataaag tctaataaag
     7561 ttgattctaa tgcaagtaaa gaattaaaaa aagtaagacg aaatatagaa attacagaag
     7621 caaagataaa agatagatta aataaattta taacatcaac tgtaaataaa aagtatatac
     7681 aagagtttat aataagtaaa agaaatgata ggtatgtaat accaattaaa tcttcttata
     7741 aaaatgaagt tgatggtact atattagata catcatcaaa aggaaatact gtatttattg
     7801 aacctattag tgtttcaaat ttaagtacag agttaattat gctaaaagct gatgaaacta
     7861 ttgaagaata caaaatatta tcttatttaa cagaacttat atttgagaag ataagtcaga
     7921 taaaattaaa tatagagata ctttcagaat acgatatggt atttgcaaaa gctaaatata
     7981 gtcaaaaaat aaaaggaatt actccaaaaa taaataataa tggctatata aaaataatta
     8041 aaggtaaaca tccacttcta acaggagatg cagtaccttt agattttgaa ataggcaaaa
     8101 attatagaag tcttataata acaggtccta atgcaggagg aaaaactgta acccttaaga
     8161 ctgttggtct gctaacttta atggttcaat gtggatttga tatttctgca aaagaaggta
     8221 gtgaatttag tgtatttgaa aaagtttttg tagatatagg ggataatcaa agtattgaaa
     8281 atgccttaag tactttttca tcacatataa aaaacattgc agagattatg aacttatcaa
     8341 ataattctac ccttgttctg tttgatgaaa taggaagtgg aactgaacct aatgaaggag
     8401 ctggattagc aatatcatta ttagaagagt tttacaaaat gggatgtata actatagcat
     8461 caactcatta tggagagata aaaaagtttg ctacattgca tccagagttt gaaaatgctg
     8521 gtatgatgtt tgataaagaa acgttagaac cattatataa gttaactatt ggaaaatctg
     8581 aagatagtaa tgcccttttt atttctaaaa aaatgggaat aaaaaataga gttttagata
     8641 gggctaaaga atatgtaatt gatagaaact acaatttaga tttagttaaa agaagtaagc
     8701 tacaatctaa tgttgaagac aaaaatagtt ctgaagtcat tttaaactat acagattata
     8761 atgtagggga taaggtaaaa ttactagatg aagatgattt tggaattgta tataagccta
     8821 tagacaagtt taataatgta gaagtattgt ttaatgataa ttttatttca gtaaatataa
     8881 aaagattaca acttagcata aaagcatcag aattgtatcc agaagggtat gatttagata
     8941 cattatttac taattttaaa gaaagaaaat tggaaaaaga tattcaaaga ggttcaaaaa
     9001 aagctcttaa aaagattcag aaagaaatta gagagaatag aaaataaata taaaaaacat
     9061 aaaagaaagc aatctaaaga gcaaatttta tctttagatt gctttcttat taacatatct
     9121 taataaaccc aatgactaac aagattaagc cacttagcca agataagttt aaatttatgt
     9181 ttttaacaaa catatttcct agtaaacaac caaataatac tgcaattata tttgatatta
     9241 aagaaaatag aataacctgt atataattca cacctacaag accactacca aaaccaatag
     9301 ctaatccatc taaacttaaa gcaagtgcaa gtgtaaatgc ttcagcagat ttaagcactt
     9361 tagatttgct tgtatctaag acgtcaggtt caatacttac attaaaattt aatttaaggt
     9421 ctaacatatt aaaattataa ttctttgagt gtatagattt tttcttaaaa taagatttaa
     9481 aaaagctttc aattagtcta gcgcttccta aaaaaaataa gataaagaaa catattagca
     9541 ttgttatatt ttgaggtaaa aagtctctaa ctattgtacc tgcaaaaagc gatattgcca
     9601 aaatagctga acaaactacg ctgataatta tatttgaata taagggaact ttaactttat
     9661 ttacgccata tccaaagctt gtcacaaagg catctataca aagagaagta actaataaaa
     9721 ttgattcaat cacagtagat taacatcctt tctcttaaat tttatactta tattagttat
     9781 agtattgtaa aaatggataa attgttacag ataatccaat aaattgttga taaaatttca
     9841 acatcataca acacctaaat aaaaaaagaa tcatatcata taataatata aaaccaatca
     9901 taataaagca ggtgaaaata atggaacaac atagcaaggt tgggaatttc ggtgatttgt
     9961 tgaagatttt attagatgaa aaagaattat caatgggaga attaagtaaa aaaagtggaa
    10021 ttgataaatc tactatatct cgtatagcta ataataaaca aaagccaaat ataaatcatt
    10081 tagaaaagat ggcaatacat ttaaatatta acctagaaga actgttgaaa gcttcaggat
    10141 atgaatttaa aaacaataat aatcaaatat ttaatacaga tagtgatttt agtaattttg
    10201 atgatattct tggatttgca aatttaatca atgataagaa ctttaatgga aatattgaaa
    10261 aagagttaag taagtgtaag ttgtatgtcc aaacagatga aggtaaaaaa ttactttttg
    10321 ataattttaa taaaaaaata gattctattg aaaaacaagg gcaattcaca gatagattaa
    10381 gacaaatgta tgctgatttt tgtacagacg gacttacaat aaagaaatat ttattgattg
    10441 gaagtatgct actttacttt gttatatcaa cagatgtaat tccagatttc gtgttcccaa
    10501 taggatttat ggatgattta gtggcattaa atatagttac aaaattactt aaaaatgata
    10561 agtaagtttt agaatcatat taaatatata aataaaaata tataaaagtt attatttttt
    10621 ataagaaaca aattaaaagt acaagaaaca gattaaaaat ttaaaactaa gaatctaata
    10681 tatattttgt aactatttaa gtatataaaa taaaataata tccaaactaa tacaaaatag
    10741 taataattgt tttgtactgg aggatattta tgaattgtaa atggatatct aaaatagatg
    10801 agagaaaaaa atgtgataga gaagcagatt cttctggata ttgtatcttt cataaagaaa
    10861 ataaatctga tgaagagata cagttgatgg tagatactct taataaagaa gaaataagtg
    10921 aatttaatgg atttgtgttt gaaaatgaat ttaatgctga agaaatatta acttacaatt
    10981 ataaaatact ggatttttct gaatccatat ttaaacaaaa agcaaatttt aaaaaatata
    11041 tttttaaaaa gaacattatt tttaattata cagaatttaa agataaagtg ttatttaatg
    11101 gttgtgtatt cttagaaaac tgtgatttta atagaaccat atttagtaaa gactatataa
    11161 atgaccgtat atttgaaaaa gtgaaattta aaggttctga tttgatagtt aataaagtag
    11221 aaaattttcc acgaatggat ggaataattt ttagtatgtg tacaaagttt gtgttaaaga
    11281 atgttgaata tggaaaaagt gagtatgagc atggcaagat aaattataga atagctagaa
    11341 atcaagcaac aaaaattgga gaatatgaga tgataggatt ttactattat aaggaaagaa
    11401 tttatagtag taaaataatg aaacgttcta attatcctac tttcagtgac tatttggttg
    11461 aaaaattttt tgaccaaata gcacgctaca cgacaggtta tggagaaaag ccatggaata
    11521 tattattagt gattatagtg ataataagtg tttttgcctt attatactta tttgtaggta
    11581 tagaaagttc aaatagtaca cttgtagctt tagatataaa caatataggt gattactcac
    11641 taagtgagat tttcagaatg tatatggatt tatggtattt tagtatggct acatttagta
    11701 cagtaggata tggagatatg gttgctacaa gtttaattgg gaaagcatta gcaggcatag
    11761 aagtattttt tggagttaca ataggagcaa tttgggcatc tgtgattatt aaaagaatga
    11821 taaggtaata tctgtttaaa tatagatagg tattaattac tagatataaa ttggactaaa
    11881 tagggaaaat ttaaatataa atatacttaa taatccaaga aggtgaaaaa tttgaaaaat
    11941 gcactaaaaa aaattatatc attagccatg attctagttt ttataatacc aaattattca
    12001 tatgcagatg ataaatctgt agacaaatat tcaaaaacat ctatacttat agaccaagaa
    12061 acaggtagag ttttatatag taaagaacct gatatgaaag taccactagc tagtttaagt
    12121 aagatgatga cttttttact tgcaatagaa gctattgaaa ataaagaagt aaaagaaact
    12181 gataaggtaa agatagacaa atctattgca tcagtaaaag ggtcaagtta taaacttaaa
    12241 gatggtgaag aagtgccact tatagaactt atgagaggtc ttatgattgt atcaggaaat
    12301 gatgcagcaa ttgctatagc caaacatatt tgtaaaacaa aggaagcatt tgtggataga
    12361 atgaataaaa aagcaaaaga aataggaatg agcaatacac attttctaaa tccaaatggt
    12421 cttccaatat acgatttaag taatcccaaa aaaccagcaa aagaaaatat atctactgca
    12481 aaagatatag ctattcttgg aaaatacatg tttgacaatt atgaaaaaca agtaacagct
    12541 ataactgata tggagacata tacaaattca gatagaagtt ttgaaaaaag caatacaaat
    12601 gcattattga gaattatacc agaagtagat ggtataaaaa ctggatatac tggcaatgct
    12661 ggatattgct tatcattttc tatggttgta aataaaaatg ataaaaatga gaaaaaacgt
    12721 agagtaatag gagttgtatt aggtactaat cataaaaata aaagaacatc tgcttcatta
    12781 gctctactaa aatatggaaa agaaaatttc aatatgaaaa aagtaattga taaagatagc
    12841 tttattggta agaaatatat aaaaggaatg aaagagctag aggttacatt aaaagcaaaa
    12901 gatgaacttt atacaatatt aaaagatgat gaaagtataa aatcagaagt taagattaaa
    12961 aatatagagt accctgttaa aaaaggcgat actatgggta ttataaaata ttatactagt
    13021 tcaggtgact tacttggaag tgtagatata gtaagtaata atagtataga taatgtaccg
    13081 ttaaaaacga aattaaaaat gaaatttgca aattaaattc aaaaaatggt ataaaataca
    13141 caaaaaaata taaaaataga aaatatggaa aaaatattgt tatttcttca cctctgtgat
    13201 atactactag aggtgaaaaa ataaaataaa tcacacacag cattgggatc cttaaaaggt
    13261 gcctattaca tggtttttta gggagaagat caagctggag gtataaaacc aggaggtaag
    13321 atatgtcagt aatatcaatg aaacaattat tagaagcagg tgttcacttc ggtcatcaaa
    13381 caagaagatg gaatcctaaa atggctaagt atatattcac agagagaaat ggaatatata
    13441 taatcgactt acaaaaaaca gttaaaaaag ttgaagaagc ttacaaattc actaaagaag
    13501 tagctgaaac aggaaagcca atcttattcg taggaactaa aaaacaagct caagatgcga
    13561 taaaagatga agctgaaaga tgtggaatgt actttgttaa tgaaagatgg ttaggtggaa
    13621 tgttaacaaa ccacaaaact ataaaaacaa gaataaacaa attaagagaa ttagaaaaaa
    13681 tggaagaaga aggcgtattt aatgttcttc ctaaaaaaga agttataaaa ttaagagctg
    13741 aaaaagaaaa actagaaaaa tacttaggtg gaataaaaga catgcctgaa ttaccaggtg
    13801 caatgttcgt agttgaccca agaaaagaaa acatagcaat tcaagaagct catagattag
    13861 gtataccagt agttggtata gttgatacta actgtgaccc agaacaatta gactttgcta
    13921 taccaggaaa tgatgacgct ataagagccg taaaattaat aactggtgct atggcaacag
    13981 ctgtaattga aggtagacaa ggtgctgaag aagaagtagc tgaagaccaa gaataataat
    14041 ttgacaatta aatggtaagg gtcgccctta ccatttataa tatggaatag aataagtagg
    14101 aggaaactta aaaaatggct aatataactg ctcaaatggt aaaagagtta agagaaagta
    14161 caggcgcagg aatgatggac tgcaagaaag ctttacaaga agctgaagga aacatggaaa
    14221 aagcagtaga tttattaaga gaaaaaggat tatcaaaagc tgctaaaaaa gctggtagag
    14281 ttgctgctga aggtctagtt gcaatagaaa tgaatgatga taatacagtt gcttctatgg
    14341 tagaagttaa ctcagaaaca gatttcgttg ctaaaaacga agacttcaaa gtgttcgtta
    14401 aagatgctgc ttgtatggca ttagctactg ataaagaaga tatagcttct ttattaggtg
    14461 aaactcataa agagggaata actttacaag aagttttaaa taatagggtt gcaaaaatag
    14521 gtgaaaaatt agactttaga agatttgcta aagttgtaac taatggacag gttgctggat
    14581 acattcacgg tggcggaaaa ataggtgttc ttgttgaaat ggaaacagaa gctagagatg
    14641 ctaaagtttt agaattaggt aaagatgtag ctatgcaagt tgctgctatg aatcctaagt
    14701 atgtttcaag agatgaagta gatgctgaat acatagcaca tgaaacagaa gtattaactc
    14761 aacaagcttt aaatgaagga aaaccagcta atatagttga aaaaatggtt aaaggaagat
    14821 tagaaaaaga attaaaagag gtttgtttat tagagcaaac tttcgttaaa aatccagata
    14881 taactgttaa acaattagtt gctgatgttg ctaaggcagt aggttctgat ataaaagttg
    14941 ttaaagttgt gagattcgaa gttggtgaag gtatacaaaa gagagaagaa aactttgcag
    15001 aagaagttgc taagcaactt aagtaataac caaagagaga acacgcaggt gttctctttt
    15061 ttaaaaaata gaaaaaatgg atatgaagga ttgaaatagg aggtttctga tggataaacc
    15121 aatgtacaaa agggtattgt taaaattaag tggagaagca ttagcaggag aaaaaggttt
    15181 tggtataaac aatgatgtag ttaatgatat tgctattgct ataaaaaaaa tacaagaaat
    15241 aggtgttgaa gtagctgtag tagtaggtgg aggaaacttc tggagaggta gaactagtga
    15301 aggaatggat agaactactg ctgactatat aggaatgtta gctacagtta tgaatgcaat
    15361 ggcgctacag gatgcactag aaaatataga tgttgctaca agagttcaaa ctgctataga
    15421 catgagacaa attgcagaac catatataag aagaagagca gttagacact tagaaaagga
    15481 aagagttgtt atatttggtg caggaacagg aaacccttac tttacaacag atacaactgc
    15541 tgctttacgt gctgctgaaa tggaagcaga agttatcttg ttagctaaaa atgttgatgc
    15601 agtatatgac aaggatccaa aagtacatgc agatgctaag aaatttacag aattaagtta
    15661 catggaagta atacagaaag aattaaaagt aatggattct acagctacat ctttatgtat
    15721 ggataataaa attccaataa aggtatttga gcttacaaca gagaatataa taagagcagt
    15781 aaaaggcgaa aatataggaa ctactgtaaa ataatgaaga atgaaggagg agtaaaatta
    15841 tgaaactaga aatacataag caattagaag agaaaatgaa tgggacaatt gatgctttaa
    15901 agtttgaatt tggaactata agagcaggaa gagccaatgc acaaatgcta gacaaaataa
    15961 gagttgatta ttatggaact ccaactccaa taaatcaaat tggtgctata tctgtaccag
    16021 agccaagaat attaatgata tctccttggg ataaatctgc tatgcatgaa atagaaaaag
    16081 ctatagctaa ctcagactta ggattaaatc catcaaatga tggtgaagtt ataagacttt
    16141 ctgtacctgc tttaacagaa gaaagaagaa aagaattagc taaaaaagct agtaaagctg
    16201 cagaagactt taaagttaga ataagaaatg aaagaagaga cgccaacgag aaaattaaaa
    16261 agatggaaaa aggcggagaa ttaactgaag atgaattaaa aaaagcacaa gatgaagttc
    16321 aaaaaatgac tgataaattt ataaaagaaa tagatacatt attatctaaa aaagaaaagg
    16381 acataatgga ggtataatat tgaaaaaata ttataacctt ttaggtcttc atgtagatga
    16441 ggtaaaacaa tattttgatg agcaagatat aaattatacc ataaaatcta ttgaaggtaa
    16501 aaaggataaa gataaacttg tagttcctag agttataaaa atatctgaga taggtgatag
    16561 tgtagaactg ataatcacat acttttctga ctccttagtt taaggagtca gaacaatatt
    16621 ggaggcaaaa tatgaataac aacattatat atgatataga cttaaataac atacctactc
    16681 atattgctat tataatggat ggaaatggaa gatgggcaaa ggctagattt cttccaagaa
    16741 ctgcaggaca taaggctgga gtagaaacta taagagatat agtaaaagaa tgctcaaaat
    16801 taggtgttaa gcacttaaca ttatatgcat tttcaacaga aaactggaaa agaccgaaac
    16861 ttgaagttga cacacttatg aacttattat ctacatactt aagaaatgaa atagctgaac
    16921 ttcaccaaaa caatgtaaaa gtaactgcta taggagatat aagtgcttta ccaaaaactt
    16981 gtataagaga attaaactca gctaaggaac tgacaaaaaa caatacagga gttaacttaa
    17041 atttagcatt aaactatgga agtagagcag atataaaaaa tgctcttata gatatagtaa
    17101 aaaattgtga aagtggtaaa atagatataa acaatattga tgaagatatt ataaaaaact
    17161 atttaagcac aaagtctata ccagatccag acttggtaat aagaacaagt ggtgaacaaa
    17221 gattgagtaa ctttttactt tgggaggttg cttattctga gttttacttt actgatatac
    17281 actggcctga ttttaaaaaa gaagaactac agaaggctat atacgtatat caaagaaggg
    17341 atagaagatt tggaggactt aagtaaggag attattttat gcttacaaga attattgctt
    17401 cactagctct ggttccatta tttctatttg ttgtatatgg agggattcct ctatatatag
    17461 ctgagacagc aatagtttat attgcattac atgagtttta taaggcattt aaaataaaag
    17521 atgtaaatcc aatattcatt ataggatatt tattttctat ttatttagct gtaaaaaata
    17581 tttttaattt accattggaa tatacttatg cagtaatttt tattttattt ttagcaagta
    17641 taatttatat gttgatgggc aaaaacaatg ttatagatgt ttcgattacg tttctaggag
    17701 ttttttatat aggagtattt ttagatttta taattataac tataaatgaa tttgaaaaag
    17761 gcagtatata tgtatggctt atatttgtga tatcttttat gacagatata tttgcatatt
    17821 ttagtggtta tttacttgga aaacataagt taattcctaa agttagtcct aaaaagacta
    17881 tagaaggagc tattggaggg ataataggaa gtacactttg ttgtatttta tttggatatt
    17941 tatttggtat tgatttgctt caattagcga taataggaag tataggaagt gtaatagctc
    18001 aattagggga tttatttgca tcttctataa agagatatgt agggataaag gattatggaa
    18061 agataatacc tggtcatggg ggtatattag atagatttga tagtgttata ttagttgcac
    18121 cttttgtata tagtgcgatt aaatttttta ttagataaag attggttgga tataaaagag
    18181 caatctataa ttgctagtaa tgaaaatagc tatgtaactt ttaaaagtag agatattctg
    18241 cagttattag ttacatggct attttcattg tttgataatt tagaaaatat taaggaaatt
    18301 ttataaaatt atagagtact ttatagaaat atactatatt tgtagtatga atatttta
//
LOCUS       DAMJVM010000069        17970 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.072, whole genome shotgun sequence.
ACCESSION   DAMJVM010000069 DAMJVM010000000
VERSION     DAMJVM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17970
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.072"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            78..803
                     /locus_tag="PC523_002521"
     CDS             78..803
                     /locus_tag="PC523_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2476032.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNTGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGANFRFCDLS
                     NSDIRGANLENSNLEYATLNGVISDKSTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAKDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
     gene            complement(1013..2209)
                     /locus_tag="PC523_002522"
     CDS             complement(1013..2209)
                     /locus_tag="PC523_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2476033.1"
                     /translation="MLKTNNLSVGYNNKVVVSDINIEVKSGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINHISRKALSKKMALVLTNRLLADLMTVQDIVNIGRYP
                     YTGFFGNLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKNLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDENIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFESISDESFDLAIRNLNASKIIIDT
                     GFSVGETNKRNIDIVKEALKLDKKVYSFRNRDESKKYYDSLDNKIEHVDKVSQIVDSI
                     NMNNLL"
     gene            complement(2212..3237)
                     /locus_tag="PC523_002523"
     CDS             complement(2212..3237)
                     /locus_tag="PC523_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2476034.1"
                     /translation="MDFINKMRIRNTTIFIVLIISIILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGSCLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVRNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILITVGVLGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIKFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQVS
                     KLIFKTSDNRILIPVVIFLGSIVTALCDLISRVVLSPVELPISTITSLLGAPIVVMLL
                     MKRKDRA"
     gene            complement(3309..4439)
                     /locus_tag="PC523_002524"
     CDS             complement(3309..4439)
                     /locus_tag="PC523_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2476035.1"
                     /translation="MKLKSNVLKQFFLIILAIVMVGGLIGCSKNDKENKQVSNGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDIA
                     TSELNKTVQKIEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     regulatory      complement(4669..4850)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(4903..5796)
                     /locus_tag="PC523_002525"
     CDS             complement(4903..5796)
                     /locus_tag="PC523_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HDF2476036.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDREGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVDKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKAEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMDKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     gene            complement(5876..6778)
                     /locus_tag="PC523_002526"
     CDS             complement(5876..6778)
                     /locus_tag="PC523_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF2476037.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNISSEDIIKLKTIINESTFYNYDVFKRERCTIHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDNSFEDPQKTVYFTKRVIKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSSVPFPIK
                     LKVCSRVNGEERQSSNTRLFISDVSDIVSELSKGMTLESGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            complement(6828..7988)
                     /locus_tag="PC523_002527"
     CDS             complement(6828..7988)
                     /locus_tag="PC523_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2476038.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQINKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            complement(8143..8448)
                     /locus_tag="PC523_002528"
     CDS             complement(8143..8448)
                     /locus_tag="PC523_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2476039.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTIKTITALDDI
                     TIYHKLAIKDIAKGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            complement(8546..9520)
                     /locus_tag="PC523_002529"
     CDS             complement(8546..9520)
                     /locus_tag="PC523_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDF2476040.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            10110..10886
                     /locus_tag="PC523_002530"
     CDS             10110..10886
                     /locus_tag="PC523_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDF2476041.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADAINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPE
                     GLKGFTEKSITDFDVLYDQLLEIQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            11218..12363
                     /locus_tag="PC523_002531"
     CDS             11218..12363
                     /locus_tag="PC523_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2476042.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKNSIKSLGTKKAFVVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYNEVKQNPTVTNVNKGLEILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIVAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDNNSLSTITVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSSPSKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENAMKDACAGANPVFFDKKKIIELFMKIS"
     gene            complement(12544..13413)
                     /locus_tag="PC523_002532"
     CDS             complement(12544..13413)
                     /locus_tag="PC523_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2476043.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDNKTGYVFNYFLSTDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPDTPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(13529..14242)
                     /locus_tag="PC523_002533"
     CDS             complement(13529..14242)
                     /locus_tag="PC523_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HDF2476044.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELIRLIKAKEEIEWLLNRDYKIDP
                     IVNFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKENTVNIDGFNLIISI
                     EVALSGGTLVIGSDGNIRDLAGLRGTYKIINKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKDCNIVNLNK"
     gene            complement(14611..15999)
                     /locus_tag="PC523_002534"
     CDS             complement(14611..15999)
                     /locus_tag="PC523_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2476045.1"
                     /translation="MKKIFDAIIIGFGKGGKTLAGNLANRGLKVALIEKSNKMYGGTC
                     INVACIPTKSLENSANNVKTKNINSWDEVQVEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENKIYELVADNIFINTGSRPFIPNIKGIENKNVVYD
                     SESLMNLRTLPKKMTIIGAGFIGLEFAGIYSSFGTEVTILNSNNGILPNEDVEDSEEI
                     IKLLEKRNVKIINNANIKEIKEVSKLAIVEYEVDGKSKELASNIILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIIINQLFGD
                     KTRTTSDRKNIPNSIFISPAFSRVGLNVKQAKEKGYKVLVANMPIEAIPRAKQIGKTD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPSMIKEV"
     gene            complement(16120..17397)
                     /locus_tag="PC523_002535"
     CDS             complement(16120..17397)
                     /locus_tag="PC523_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HDF2476046.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMRAITAFLLGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNMSQNSKIESKQEENKRENSTNKEERSEGNDSQKDSNNENNDEG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPHIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            complement(17534..17869)
                     /locus_tag="PC523_002536"
     CDS             complement(17534..17869)
                     /locus_tag="PC523_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2476047.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQRDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNIVESTYFLSESGLLLLPVMEKMIMWS
                     NKNLNCDKY"
BASE COUNT         5963 a         3125 c         1976 g         6906 t
ORIGIN      
        1 tacttattgt tatataatta aatatatgac tattttgcta ctacatatat aaaaataaat
       61 ttgtgaaggt gattttaatg aaaactattt ctaaacaaga attagaaaaa acacttgaga
      121 aacattattt atggcttaaa aacaataata ctggagaaaa ggcagattta aaagaattaa
      181 gattggaaaa catggattta agaggatact gcttaagtaa tattgacttt tcgtggtctg
      241 accttatcaa tttaaattta gaaaaagcag atttagaaaa ctctattttt aataattctt
      301 atctttcgga ttgttcatta aaaaattcta tattaaaaaa tgcagactta agtggtgcaa
      361 attttagatt ttgtgactta tctaattcag atattagagg agctaattta gaaaattcta
      421 atttagaata tgctacttta aatggagtaa tatcagataa aagtactcgt ttttttaaat
      481 tgtactgtcc tgaaacagga gcatttatag gctataaaaa atgttttaat tttagaatgg
      541 tacaattatt aatacctagc gacgcaaaaa gagtatctgc tacatcaaat gcttgtagat
      601 gtgataaggc aaaagtacta tcaattacta gtatagatgg taaagaatct tttaaaagcg
      661 ctcgtgcata tgctgatgaa aattttatat atagagtagg agaaacgatt gtagctaaag
      721 attttaatga aaatagatgg aaagaatcaa ccacagggat tcactttttt ttaactagag
      781 aggaagctat tggttatctg taaagtaact tatttttata acgacacata taaaatacat
      841 aaatgataaa aaaaggtctg tctcaaaata gagataagcc ttttttcatt gttgaaataa
      901 tgaaaatata tttaatttca tacaaaaaga gtgcctcatg aactaaaaag tccattttga
      961 gccactcatc tttttatatt ttgtaattta ctggtatatt tgattttaaa ggttatagta
     1021 aattattcat attaatacta tctacgattt gagaaacttt atctacatgt tctatcttgt
     1081 tatccaagct gtcataatac ttcttactct catctctatt tctaaaagaa taaacttttt
     1141 tatccaattt taaagcctct tttacaatgt ctatattcct cttgtttgtt tctcctacac
     1201 taaatcctgt atctattata atcttagaag catttaaatt tctaatagcc aaatcgaaac
     1261 tctcatcact tatcgactca aatggatttt ctgtaaacat ttttatcccc atagttctac
     1321 caatttcata atcaatatca ttttcatgta ttattccaga atatagacct atacctttct
     1381 tagtaaaagc tctataaatt ggtgtcgctt tacctccacc accaattata aaaacttcat
     1441 ttttagattt attactaatt tctactgaac ccaaaagact attaaaattt ttatcatcta
     1501 attcataaag tgaatttata atattctcat caactacatc ttcaggctga ccataagcta
     1561 tgactttatt attttttata agagcaacct tatcacaact ttttaatgct atgtctattt
     1621 catgcaatga aagtattact gatattgact tttctttact taaatttttc agtatattta
     1681 tgagttctaa tctatgtttt atatccaagt gagttgtagg ctcatccaat atgattattt
     1741 ctggttcttg aacgagagct cttgcaacca ataccttttg tttttctcca tcacttagtt
     1801 catcaaaata tcttttcttt aaatgtaagg catctacaga ctccaatgct tcatctacca
     1861 ttattaaatc tttcttagat aaattaccaa aaaatcctgt atatggatac cttcctatat
     1921 tgactatatc ttgtacagtc attaaatcag ctaataatct atttgtaagt actaaagcca
     1981 ttttttttga taatgctttt cttgatatat gatttatgtt tacaccgttc aaatatatct
     2041 caccttttat tggtgatata agcttagata aggacctcaa tagagtagtt tttccagctc
     2101 cattacttcc caaaagacat agtatttctc cactttttac ttctatattt atatcactta
     2161 ctactacctt attattgtat cctacactta aattattagt tttcaacatg tctaagccct
     2221 atccttcctt ttcatcaata gcatcactac aattggagct cctaataaag acgttatcgt
     2281 acttattggt aattctactg gcgataaaac tactcttgat attaaatcac aaagagctgt
     2341 aactatactc cctaaaaata ttactactgg tatcaaaatt ctattatctg atgttttaaa
     2401 tatcaatttt gaaacttgtg gcactgcaag tccaataaat cctataggcc ctgcaaatgc
     2461 tgatactatt gctgccaata tgctagatat aaatactatt aaaactctaa aaaattttat
     2521 atttatacca atactttttg catactcttc tcctaataag aaagcattta aaggttttat
     2581 tatgaataca gtcatcaaaa gacctaacac accaactgtt ataagtattt ctaccttttc
     2641 ccatgtaaat ccagaaaaac ttcccataga ccacatagta aattccttta atttatcact
     2701 atttgcaaat gattgcaaca catttgttat accactacac acatatccag tcataattcc
     2761 tattattaaa agagttgtta tatttcttac cttatttgca aatagaatga ctatagacat
     2821 tacaattaaa gctcctataa atgctgctac aaatatactc attgaattta cactctcaaa
     2881 gcccaatgtg tatcctccca aaatcatgag tgctactgat aaagtagagc ctgaagatac
     2941 ccctaatata tatggttcaa ctattgggtt tttgaaaaat atttgtaata aaatacccga
     3001 tactgctaaa catgacccac ctaccatggt tgcaagtact ctagggattc ttatattgta
     3061 tattatttta taatgttctg tattatccat tccccttaat acaacatcta ttacttcttt
     3121 aaattcaatt tttacagaac caaatgctac acacactaaa aaagtaagta taatacttat
     3181 aattagcact ataaatattg tagtattcct tattctcatt ttattaataa aatccatttg
     3241 tatactcctc ttagcaaagc tatattaact aaaatctata aaaccaatct ttttttgtcc
     3301 tttctgtact atttatcata atttatataa tgtttgattt tttcacctgt agtacctggg
     3361 tgaaacatct caactaaatc tactattaat tcgtccgtct tatctactga ttgataataa
     3421 tctggagcaa acacccataa gtttccattt tttacaacat ccaaatcagc caatacaggt
     3481 gcactagatt ttatagaatc taagtttggt gcataatctg aacttgatga gtatacaaat
     3541 atatctgcat cctttgcaac ttcatgcaat tgttcaatac tcactcgtcc ttcatttgaa
     3601 tctattgaag cacatgcatt tattccacca gccattttta tcatattatc cacatatgaa
     3661 tccttaggcg ctatataagc acttccttca tatacagatg cccatacaac tttaggttta
     3721 gctttgtttt ttattttttt acttacttct tctatctttt gtacagtttt atttagttca
     3781 ctagtagcta tatcttcttt atcataaaat gcagctatta aacttgtcca ctccattctt
     3841 ccaaatggat tttgctcttt atactcattg tctacaacat aattaagacc taattctttt
     3901 aatttgtcaa taaattttgt atcagcctct gataagccac tactcactat agttaagtca
     3961 ggatttaaat ctactatttt ttcataatct ggtgctaaat ttgaaccaac ataatgtatt
     4021 ttcccatctt tcattaaatc tttaatttct ttaatagtcc aatactgctc ttctgttgtt
     4081 actgctttta tactatctag ttcatctatt gttctcagag aacaagcctg tgttgtggat
     4141 gctattaata cattatccac aggcgttcta actatattag cgtttttata tttttcagga
     4201 attttaattt tctttggtat taaaatcagt tctcgattgt ctccatcagt tactttttta
     4261 attccatctt ctaaatcttc aacactaaat ccactagcat attttaattc taatttctta
     4321 ccattactaa cttgtttatt ttctttgtca tttttactac atcctataag acctcctacc
     4381 ataacaattg ccagaataat taaaaagaac tgctttaaaa catttgattt aagtttcatc
     4441 tctttcactc caatatctta ttttttatga gtggctaaaa aactataata agatatgctt
     4501 tttaaagtat atcttattat agttttccta aagtcatctt taataagtat aaccttttaa
     4561 taaaataaaa aacttctcct agtttaataa ggagaagttc atatatcaac tttacaaaaa
     4621 atgaattgat acttcatttt attataaatt acaatctcca ccctaaattg aacttatata
     4681 atactgggca ggtctcctga ctcacggctt ttcctccatc acccttccca aaatttttat
     4741 aagtttcagt ggattttgat ttcgtatccg tatacagtag cgggggctgt agtaaatttt
     4801 catcactttc cctattaaac taatatagta ccctagtatt attttaattt aatttttatt
     4861 aagataattg taatttattg ttgacctctt tgcaaggtcg atttaacata tatttaattc
     4921 attcatcaat tttttaattt cacatacttg tttttcattt ggttttacaa acggagcagt
     4981 catataatcg cttatttgta gccctctaat attcatggct tttttcattg ctggtataaa
     5041 tggattggtt attaagtaaa aatccattgc tttgttaata tatttttgaa tttctataac
     5101 cttatccata tctctattat tgtaagcttc agcccatttg gaacaaactt ctggaattag
     5161 atttgatagt ccccctatac aaccagctcc tccagacatt agattatgta taaagttttc
     5221 atcataacca ctataaacct taaattctgg gaactctgct ttcatagtat ttattaaatc
     5281 tctggtatgt cccatcaaag ttactgtatc cttatatcca actatgtttt tatgttttct
     5341 tgcaagtctt aatgtaagtt ctgggcttaa atcataacca gttctatctg gataattgta
     5401 taaatatatt tcagcttctg tattcttagc tatttcgcta taaaataact caatgctatc
     5461 atcatcaaga gagaaataat aagggcttat tatcataact ccatctgctc cactattatg
     5521 tgcatagttt gaaagttcta tagattcatt tatacacata cgacttgtac ctataaatac
     5581 ttttactctt ttatctacat gactagttac taaatctata agttcttttt gcatatccat
     5641 agacatatta aagaactctc cagtacttcc cattatgact agtccatcta cacctccatc
     5701 tataagatgg tcgtacactt ttttattacc ttctctatct aaatttcctt cttcatcaaa
     5761 tattgtaaca actggtgtca aaaattctgc tttcataatt taaactccta aaatttattt
     5821 taatatgttc attaatacat cagatttagc tttaatatta attatattaa aacatttact
     5881 taatgtaatt ttttaaaatt ccaatactct caatctcaca ttcaaccaca tctccactac
     5941 tcatatatcg aggaggtgaa aatcccatcc ctactccaga aggtgttcca gtagctataa
     6001 tgtctccaga ctctaaagtc ataccttttg aaagttctga aactatatct gaaacgtctg
     6061 atatgaataa tcttgtatta gaactttgtc tttcctcacc atttacccta ctacatactt
     6121 taagttttat aggaaaagga actgaacttt tatgtaaaat acatggacct aaagaggtaa
     6181 atgtatctaa tccttttcct ctaaaccatt gaacatgttc tttttgcaac tttctagcag
     6241 aaatatcatt tataacagtg taaccaaaga tataatcttc tacctcttct tttctaatat
     6301 taacacctgt tttacctatt actactccaa gttcaacttc atagtctagt tgattattta
     6361 tctcaaaatg accatctatc tcatcttctg gacctattgc ctttatcaca cgttttgtaa
     6421 aataaactgt tttttgtgga tcttcaaaac tattatcaaa gtgagtttgt gtttcttcaa
     6481 gatggtcttt atagttcact cccacacata aaatatcatg tattggtttt tctataggaa
     6541 catgtatagt acatctctct cttttaaaaa catcataatt atagaatgta ctttcattta
     6601 taatagtttt caacttaata atatcttcac tagatatatt ttgaattaat tcatttaaat
     6661 ctttaaagtt tctactaaga tttaaagaat taacatctat aataaataat tcatctttag
     6721 aaaaaactcc agttctttta cattcatttt ccccttcagt aaaactgaca aacttcataa
     6781 tctcaccctt tatcaaaatt ctatgtattt agtttcttac ttaaagatta tacatagtta
     6841 catactctag ctattgccat ttcagtatat ccaagagatt ctgcttttga ttgttttcca
     6901 tttgctacat ctacaaccat atttagcaaa tcatctgtta gttcttccat tgtttgtgga
     6961 ccgtatataa ctggactagc atcaaaatcc atattatctt ccatatttgc aaaggttatt
     7021 ttatttccag ttatttttat tactggagct attggatttc ctgatggtgt tcctcttcct
     7081 gtactaaaca ccactatttg tgctcctcct gctaccatac cagcaacaga tgaagcatca
     7141 tttccaggtg tatccattat aacaagacct ttcttattaa tttgtttagc ataatcatat
     7201 acttcactta tttgtctatg accaccttta tgtatacagc caagtgattt ttcttccaaa
     7261 cttgttaaac caccagctat atttccagga gatggattac cttctctaac ttcttctcct
     7321 accaatttta atgaattttc atatctatgt actatatgta atattttttc ttttacttcc
     7381 tcatttactg ctcttctagc taatatatgc tctgcaccta taaattcagt agtttcactt
     7441 aaaattgatg ttgctcctaa atcaacaagt ttatcactta attcacctat caacggattt
     7501 gctgctaatc cacttgttgg gtcagaccca ccgcattcag ttcctagtat aagttcagat
     7561 attggaaatt cttcttttct aagcaaagaa gcttcttgtg ccatttctct agcataacga
     7621 acagcctttt ctatagtttt taaagttcca tgttcttctt gtattacaag agtttttatt
     7681 ggcttatttg ttctctcttt tattgcatca actaccaaat cattttggca attttcgcat
     7741 cccaaagata caactattac accatataca tttggatttg ctgcataacc agccataaca
     7801 tccatagtta attgttggtc tgaatgtact tgtgaacatc cattctgatt attaaaggta
     7861 actgcccctt gaacttgtga tgctattatt ctagttgtat ctgatgcaca cacactagta
     7921 ggcaatatta atatgtggtt tcttatacca actcttccat ctggtctttt atatccataa
     7981 aatttcatac tgatttcctc ctaaaatagt ctatatactt tttataattt tcatatatta
     8041 taatttttat tacactcaat aatcaaacaa ttaaaatcct atttaacaac cattcaacac
     8101 caaaagcaat gaatacaact tttataaatc cataaatatt atttatatat tagtcataac
     8161 ttgtttatcc aagtcctctc taacacttct aacattatga atatgcacat gttgaccact
     8221 ttttatttcc tgatttgctt ctcctatatg ttcaccatat ttagtaactt tatcaccttt
     8281 ggctatatct tttatagcta atttatgata aatagttata tcatccagtg ctgtaatcgt
     8341 ttttattgtt ttgtctttta atttaaaact aatttcacta cctttagcta ttgcttcaat
     8401 agctactgca acattatctt tttcatcaat tataactgca tttatcatta ttcttgctct
     8461 cctttataaa taatataatt ttattaatat cctaaaacct aattaaagaa tttactattt
     8521 taccaacaac tcactgtaat ataaattaag ctgttattgt accatcctca ttttgttttg
     8581 ggaacttagg acatccatac ttcttagccc accaactagt tataagaggt accaatattg
     8641 cagtgacaac tactgcggct gccacctgtg ttgtagctgt tgcaacatat ggttgccatg
     8701 atgggtcaac taatgcaaca gcagcaggta cagcaattgc atttccagca gttgtagcta
     8761 cagcccatcc tgcatatcct ggacgttttc ctataaattt atcacaaaat actataaata
     8821 tacctcctac aaatacagat attaatccaa gtagtactcc tgataaacct ccttttaata
     8881 tacttgcaag atttatgcta ctaccaagtg caaatcctac aaaaggtacg agtaaattac
     8941 accctggttc taaaaactct ttcatttttt tgtcaaaatt tcctattatc ataccaacga
     9001 gtattggcac tattgcagcc aataatgaca acaaaggtac atttgcaaga cctgaagtcc
     9061 caagagctat cagtgtaaaa aatggaccat catttaatgt aagtaaactc atagttgcac
     9121 aatctgtttc atctccatat gaagtcatca atgaaagata tatactacca ttactatttg
     9181 taactgcgct tataactgct aaagttgtaa gaccaagtac tccatccatt ccaaataact
     9241 ttcctataag tatacctatc aaagcaccta tagcaaattt agaaccaaga agtataccac
     9301 ccctttttaa aacttttggc atttctctaa attgaagctg agttcccaag cataccagtt
     9361 gaactccaat tatggttgca gacccaacac ttgaaaatac tgctgtcgta aacgaaccta
     9421 tttgtacaat atctgggcaa aaggtattaa tgaaagccgc tataaacatt ggcactatca
     9481 tcatacctgc aggaattttt ttaataaaat ccattatcat aaagttaatc ctcctaaaac
     9541 atggtattta attactctta ttaagttttt agctttaatt ctaacggccg ttttagttcg
     9601 tatttaagaa ctacgttttt aattacgaat ctttgaatta atgatagcat tttcctattt
     9661 atgagtcaat aaaaaaaata aatttttgtc aaaatttttt caacttttct gataagacat
     9721 tatgtattat caaatgactt ccttatatgc aaaaatacat agtatttaaa attatatggt
     9781 attcaaaact ataaaatttt tatacaatct aaaaaataga taaattataa aatataaatt
     9841 cataatattt tgatatttat atataaaaat aactattaca tatatttact taaattatat
     9901 aactaaatat cacttgcttt ctttaggtat attttggaaa ttagttactc cttcctatac
     9961 aacctaaaaa aatatctata ttcatctaaa ttaagagctt atttatatat aaatttacat
    10021 atttattttc tacattgtaa aatatcgtat agtgtgctaa aatcaacata ttctaatact
    10081 ttaaactaat aaaaagaggg gggaaattta tgacttcaga taatcatcgc ccaacagcta
    10141 ggatattaga tattttagaa gcattagcag aatccgagaa tggatatact cttacagaaa
    10201 tagccgatgc tatcaatgct ccaaaaagta gcatctttcc tatagttcat acacttaatg
    10261 ctagaaaata tattactatt gataaaaaca catccaaata ttctataggg atacgttctt
    10321 acacattagg gtcttcattt tttgaaaaaa gaactatttt taactatata attgatgaaa
    10381 tgactaatat agttaattcc tgttctgaaa cttgtcaact tggtatactt gacaataatg
    10441 aagttcttta tattggaaaa gtagattctc cagaaccaat tagacttatt tcttatgttg
    10501 ggaaaaaaat acctgctaat tgtacaggtc ttggaaaggc tcttttatgt gattttgata
    10561 aagaaaaact aataagctta tatccagaag gtctaaaagg ctttacagaa aaatcaatta
    10621 cagatttcga tgttttatat gaccagcttc ttgaaattca aagaacacaa atagcttcag
    10681 agtgcgagga atcaatggaa cacctaaaat gtcttgccgt accaataaga aaaaatggat
    10741 gtataagagc tgctgttagt gtaacactgc ccttatttag agctaatgac gaaaaaatag
    10801 aactaataaa aaaattactc ttaaatgctc agaaaaatat tcaatccatg atggaagaac
    10861 gtaatgaaga tattgtaata aattagatta atattctgta tacggaagat attaaaatat
    10921 aataattaaa aattttattt taaactaatg tatttatttg atttatacta taaaaacaaa
    10981 gttgctgatt taactacagt aactttgttt ttttataata ttttttatat tttcattgac
    11041 acgataaaaa tactatgtta aattctaatc ataatcgtaa ttacgaatct agttcttata
    11101 taagaaccac caaacctata tcaatctaac atacgatata ttatgggtaa attagtgaaa
    11161 attgttagat atatattttt acataaaaat caatgaaaat aaaaggagat tttagttatg
    11221 agcatttatt atgtaccacc aataaattta cttgggaaag gatgtcttaa tgaagctaaa
    11281 aattcaatta aatcattagg aactaaaaag gcatttgttg ttagtgataa atttctagtt
    11341 tcaaatggta ccgtaaaaaa agtaacagat attttatctc aaatagatgt ggattttgtt
    11401 gtttataatg aagttaaaca aaatccaact gtaactaacg tgaataaagg acttgaaata
    11461 ttaaaatcag aaaattgcga cttcgttatt actataggag gaggttctcc acaagactgt
    11521 ggtaaagcta tctctatatt agctactaat ggaggagata ttagagacta tgaaggtatc
    11581 aacaaaactt ctaaaaaatg tcttcctatc gtagccataa ctactactgc tggtacatct
    11641 gctgaagtta caataaacta cgttataact gatgaagata gacatgtaaa gatgataatg
    11701 gttgataaca actcactatc tacaatcaca gtaaatgacc cagaattaat gattagtaaa
    11761 ccatcaaatt taacagcagc aactggtatg gatgcactaa ctcatgctat agaagctgta
    11821 gtagctaatg gtgcttatga tgttacagat tcaactgcct tatatgctat aaagcagata
    11881 tttaaatatc ttccaagagc tgttaaaaat ggtaatgaca tagaagctag agaacaaatg
    11941 tgttatgctt gttttttaaa tggaattgcc tttagtaatg caggtcttgg aaacgtacat
    12001 gctatggctc atcaattagg aggtttatat gatttacctc atggagtatg taatgccatg
    12061 ctacttccaa tagttgagga ggaaaatgct aaatcatccc ctagcaaatt ccgcccaata
    12121 gctgaagtta ttggcatgga tgtaaataat aaatcagata aagagtgtgt tgactttgta
    12181 atagataaga taaaagctct ttctgaagag gttggaatcc caaaatcttt aaaagatgta
    12241 ggtgttgata atcctaattt tgaattgctt gcagaaaacg ctatgaaaga cgcttgtgct
    12301 ggtgctaatc ctgttttctt tgataaaaag aaaattatag aattgtttat gaaaatatct
    12361 taattataac aatctatccc ataaattaat tctgtatatt tataagagag tacctcaaga
    12421 tgtctttatt ttgttaatgg cactctctta ttacactttt taaatagtac tctttatgag
    12481 aagttaatat atagccttac ttatttaaaa ataattatct caataaaaaa cttaaatttt
    12541 ttcttaatgg attataacag ttgttgtatc tggtatatta tcatatatcc actttgcatt
    12601 ttccgtactt aatcttatac atccatgact aagagcttca ccaagtctgc catctattat
    12661 ataacttcct gttgaatcat atagtacaga atgatagtaa tatccacctt taattctagt
    12721 agcatactta actgaatact catccgttcc gaaagaaggt tttcttccac ttatataaaa
    12781 tatacctgtt atagttggag tgtcaggctt acctatagta cattgccact tataaagttg
    12841 tccccatcct ccattatctt tcttaaaaat ataagtatat ttatttctaa ggtctgttac
    12901 aattagataa tcgctagtac ttttaatatt attttcgtta acaaatttaa tcatatcagt
    12961 ataaaaatac ttataatcta attcatttgg tttatttcca tcagttgata aaaaataatt
    13021 aaaaacatat cctgttttat tatcatatac aaccttactc caatctccat ctacttgaag
    13081 gacttcaacc ctagattttt caggaattac tgtaattata ttggaagttg tagatttatc
    13141 ttctcttaag tttaagttac tccaagcata ttcgcttact gataccaaat ccttatacac
    13201 atatccttct tgagaattat acatcacctt aatccagtca tcttcttcat ccaatacttc
    13261 cattttagct ccctgaggta ttactgttat gatacttgaa ttagtagatt tagcagacct
    13321 caaatttaca tttgctaaag catatttata tataggtgct ctatattttt taatatttag
    13381 tttagttaat ttcacacata tccatcctct catatgtctt tttacaacat tttatgaaat
    13441 tcgatatgta ttcgttctcc tattttcaaa ctttacttgt attactaata atattgctat
    13501 atatacttat attctcattt ctatttatct acttatttaa atttacgata ttgcagtctt
    13561 taatatactc ttcaattata cctctagcca cattaaaata acttatacac ttatcaacta
    13621 taactgcatc actactaact actctatcta atttttccaa tacaacatct gcatttttta
    13681 ctaattctac ttcagtttca atcccccaag tttttgcatg ctctaatatc ctatacttta
    13741 aatttcctga attagaaact ggagcatcta aataaaattt aagattctgt gcattgtatt
    13801 tattaaaaaa tttacctatc agattcaaag cttcttctgt tttatttata atcttatagg
    13861 ttcccctaag acctgctaaa tctcttatat tgccatcact tcctattact agagtaccac
    13921 cagataatgc tacctctatg gatattatta aattaaagcc atctatattt acggtatttt
    13981 ctttgatttt atctaaagaa agcctctttg attgtcttat aatatttttt tcatcagtac
    14041 agactgccct ttttaatgca tctctttgtc ttatagaaaa ctggtatcta tcaccaacaa
    14101 aattaacgat tgggtctatt ttgtaatctc tatttaataa ccattctatt tcttctttag
    14161 cctttataag tcttattaat tctttacttg aaaaccatct tttatcacta tcatcaaacc
    14221 ctcttttaga aatcttattt atatcatttc accaccttac tttaaatatc taactatgct
    14281 atattaaata cagattattt caccttttag gataattttt aataccatta tcttatccat
    14341 ttaggttatt caatatttat aattaattat aacgcttaac tccacttaat aatacaaaat
    14401 aatagattct atattatatt tataacttta taacataaat aattagtgtt tcaaaataaa
    14461 tttttagttc ataacgtaat cttcttttat aaaaacaaat atatttttat catttaaaca
    14521 gtaaaaaaga gacttatctc tattttgaga tagtctcttt ctaaaatcta tttataatga
    14581 taattttaag ctttatatat taaacttata ttatacttct tttatcatac taggagatag
    14641 aatatcatta agagcttctg tcatagttgg atgtgcataa acacggtctc ttaagtatgt
    14701 atactctact tctaaatcca cagctaattg aatcaaatgg ataatctcac ttgaattttc
    14761 acatatcata ctagctccaa gtatcttatt ggatttttta tctatcacta tttttataaa
    14821 cccatccgtc ttgccaatct gtttagctct tggaattgct tcaattggca tatttgctac
    14881 taatacttta tatccttttt ccttagcttg tttaacatta agaccaactc ttgaaaatgc
    14941 tggacttata aatatagaat ttggtatatt ctttctatca ctagttgttc tagtcttatc
    15001 tccaaataac tgattaataa ttatgcgata atcatccaat gaaatataag taaattgtgg
    15061 acctccattt atatctccta tagcccatat atgctcttta ttagtcttaa gagtttctga
    15121 cactttaata aatcctcttt catttaattc tatacctgca ttttctagtc ctaacccttc
    15181 agtatttgcc tttcttcctg tagctacaag tattatatta cttgctaatt ctttagattt
    15241 accatctact tcatattcca ctatggctaa tttagaaact tctttaatct cttttatatt
    15301 tgcattattt ataattttta catttctctt ttcgagtaat tttattattt cttctgaatc
    15361 ctcaacatct tcatttggta aaatgccatt attagagttt aatattgtaa cttctgttcc
    15421 aaatgaacta taaatacctg cgaactctaa acctataaat ccagctccta taattgtcat
    15481 ttttttaggt aaagttctta gattcataag agactcacta tcatatacta cattcttatt
    15541 ttcaattccc ttaatattag gtataaatgg tcttgaacca gtattaataa atatattatc
    15601 tgctactaac tcataaattt tattttcagt tttaacttga actgtttttt catcaatgaa
    15661 agttcccatt cctgtgaata tagttacatt ctcatttgaa tttaatttat tatagttagc
    15721 ttctctaagt tttgtaatta aggtttcttt tttatctata gccttctcat attcaacttg
    15781 tacttcatcc caagaattta tattctttgt ttttacatta tttgctgaat tttctaagct
    15841 ttttgttggt atacatgcta catttataca tgttccacca tacatcttgt ttgacttttc
    15901 tattaaagct acttttaatc ctctatttgc taaatttcct gctaatgttt ttccaccttt
    15961 tccaaatcct attattatag catcaaatat ttttttcata taaatttcaa tcctttcttg
    16021 tattgtttat acttttacta taacaagatt acctaagtag tacaattagg cacttttttg
    16081 taaccttaaa ctgatttata aacttctact taaatacttt tatttatttc tctggttttc
    16141 aataaaggat gccatagttg ccttatccat agctcctgga atatactgag ttacatatcc
    16201 ttccttgtca attataaatg tagttggaaa agcattaatt ccatattgat atgctaatgc
    16261 tccatcttca tcaagcacaa cagggaaggt ataaccttgg tcttttaaga agttaactac
    16321 atgttccctt gaaccttctc tacctaaatt aggtgaagct actcctaaaa ttactacatc
    16381 atcattattt ttattatagt ctttatacag ctgttctata tgaggcattt cttctttaca
    16441 tggaggacac catgtagccc agaaatttaa gaatacaact ttgccttcat aatcacttaa
    16501 tttatgtgtt ttaccatact ggtctgttaa agtaaaatca atagacttta ttctatcttc
    16561 atcattagac ccttcatcat tattttcatt attagagtct ttttgactat cattaccctc
    16621 agaacgttct tctttattag tagaattttc tctcttattc tcttcttgct tactttcaat
    16681 tttgctattt tgtgacatat taaagtgttt gcttatactt ccaaatccat ttaccatcat
    16741 taatattcct gatactataa gtataatccc acctattttt ttaataattt ccatattaga
    16801 ttttatctta tctatagttt taaatagttt actgtaaaac atagctgtaa ttatgaatgg
    16861 caaaataaat ccaatggtat atactgctat taaaagattt gcactaagat gattacttga
    16921 acttgaaacc attactaata cagatgccaa tattggacca atacaaggtg tccatccaaa
    16981 actaaatgta aaacctaaca ggaaagctgt tattgctctc atctctttaa acttaacatt
    17041 aaatcttttt tctctattca atattgaaga tttaattatt cccatgtaaa ataatcccat
    17101 aataataatt ataatcccac caataaacat tattaaatct ttattttcat taaaaaacat
    17161 actcaatact tttactgatg aacccaatat aaaaaatgtt gtcgagattc caagagcaaa
    17221 aaatatagta tttttaaata aagaacttcc tataaaactc gtctttcctt ctttcaaatt
    17281 ctctacacta ctatttgaca atatgctcaa ataaataggc aaaattggca atatacatgg
    17341 tgaaaagaat gatactatcc cttctataaa cactaaaaat aaatttacat tttgcaaatt
    17401 aaaattcctc cttattttat ttccaatagt acaatctcat ttctaaatta aaatctgact
    17461 aaaaaattat gtaatttcaa tacatttttc tacttataat ttaattaact cttcactact
    17521 tccagagtaa attttagtat ttatcacaat ttaaattttt attgctccac ataatcattt
    17581 tttccatgac aggaagtaat agtaatcctg attcacttaa aaaatatgtt gattctacaa
    17641 tattattaac caactttttt tggttgacta taataccctg ttcaattaaa aaatctagtt
    17701 gttgaactaa cattttctta ctacagtctg gtatagctct ttgtaactct ccaaatcgtt
    17761 ttttatctct ttgtattttc catataatta tagccaccca ttttttacct aaaatatttg
    17821 ataaagcttc tactggacaa ttatatatca tatcatctat gttgtacatt ttattctcct
    17881 cttttcctta tttaatttat taaacaaact tttatagcaa tatatttatt aatattataa
    17941 atatacctag ctttatttaa ttaaaatttt
//
LOCUS       DAMJVM010000070        17306 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.073, whole genome shotgun sequence.
ACCESSION   DAMJVM010000070 DAMJVM010000000
VERSION     DAMJVM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17306
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.073"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(383..760)
                     /locus_tag="PC523_002537"
     CDS             complement(383..760)
                     /locus_tag="PC523_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2476048.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMEQKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFENHFKNLEEE"
     gene            complement(947..1916)
                     /locus_tag="PC523_002538"
                     /pseudo
     CDS             complement(947..1916)
                     /locus_tag="PC523_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(1950..2147)
                     /locus_tag="PC523_002539"
     CDS             complement(1950..2147)
                     /locus_tag="PC523_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HDF2476049.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            2633..3235
                     /locus_tag="PC523_002540"
     CDS             2633..3235
                     /locus_tag="PC523_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2476050.1"
                     /translation="MEVCILMGSPKKKGNTATILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKVLMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWSGKPVALITTCGYKPENGADIWENGMRRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYSILDNSIKVDNY"
     gene            complement(3511..4482)
                     /locus_tag="PC523_002541"
     CDS             complement(3511..4482)
                     /locus_tag="PC523_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDF2476051.1"
                     /translation="MKKVISIGEALIDFIPNQNGCKLKNVSGFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHSLHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSDQSCKNAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGIKVNAIDTTGAGDSYIGSFLYTLLCKQITLN
                     DIKEIKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEISEFIKNL"
     gene            complement(4508..5989)
                     /locus_tag="PC523_002542"
     CDS             complement(4508..5989)
                     /locus_tag="PC523_002542"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDF2476052.1"
                     /translation="MYKEPKYRTILESTKEELNDLRNISLNDKYKPIFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDVTHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNSIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMDKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSNKKGAIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDGKDILIFSPQGLEPKGFNYQNIYNVVYAIGNMDLDNLTFEIDIMK
                     EMEKGFDFYAPQTFIKDSQTILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELVKLRYDEISGQNTIKKNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKVENTVTLDRSNFKKVFSVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NNGEAVFTSRIFAKENSNQIKIYSDKIVCYKYTKFKLKQGIEL"
     gene            complement(6081..7511)
                     /locus_tag="PC523_002543"
     CDS             complement(6081..7511)
                     /locus_tag="PC523_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2476053.1"
                     /translation="MSAKTIDYNKVSKQLLEYIGNEQNIKGVAHCATRLRIVLDSNNK
                     ANIKAIEELEGVKGVFIASNQLQVVFGAGIVNNVYKEFSKLSGFENMSLSDVKSESTQ
                     KQNKFQQAIKSLSDVFVQIIPGLLAAALLMGITGLLGQRGIFGELSIIEMYPQLAGLN
                     RFISIASTGIFTILPLLVVYSSTMRFGGSPVLGLILGAIMLHPELTNAYSVGNGSVVP
                     EIIDLFGLKVELVGFQGGIIVALMMGFVVAKLDKFFNSKIHDMLKLFLAPICTVIVAS
                     FLLFVIVGPLGRGLANIVTSSLLWITENLGIFGYMIFAGVQQVIVITGLHHVIGAVEA
                     QLIADTGTNFIMPLMSVALIGQGGAILGYLVLNWKDIKARQIGISSFASVLFGISEPA
                     IFGVTLKYKYPLIAGCIGGAIGGAYIYMTKVTALAFGATAVPGLAIVSTQGGGHVQYI
                     LAHLVTIVVAMITTILLGKLQKKSVN"
     gene            complement(7680..8666)
                     /locus_tag="PC523_002544"
     CDS             complement(7680..8666)
                     /locus_tag="PC523_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2476054.1"
                     /translation="MKKITINDIAGLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIIAPCLDSFVKSKIMMAIDEELKELKYTSLIINTSRKI
                     RSEIDSISKLARLKVDGIILVGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIATKGYKNIVYLGVDESDISVGSNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQKSGEMLENENPDMIICATDTIAIATMKEINKRGKNIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENESAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            complement(9149..10042)
                     /locus_tag="PC523_002545"
     CDS             complement(9149..10042)
                     /locus_tag="PC523_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2476055.1"
                     /translation="MAKLTLEHIKGNTYYIQLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLIPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVKTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKNLI
                     DINKKKMEEIIDNIKKVCCEPVMIDKVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKVLQ"
     gene            10191..11051
                     /locus_tag="PC523_002546"
     CDS             10191..11051
                     /locus_tag="PC523_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2476056.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPDAFIIITDIHE
                     ALGNGFKKIE"
     gene            11320..11886
                     /locus_tag="PC523_002547"
     CDS             11320..11886
                     /locus_tag="PC523_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2476057.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLIVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNARGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLENIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            11925..12506
                     /locus_tag="PC523_002548"
     CDS             11925..12506
                     /locus_tag="PC523_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2476058.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTNGKVTNDGDFIFYNNLKHVSGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(12568..13485)
                     /locus_tag="PC523_002549"
     CDS             complement(12568..13485)
                     /locus_tag="PC523_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2476059.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     LISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVKYGKKQARKKSRSVNSL"
     gene            13615..15243
                     /locus_tag="PC523_002550"
     CDS             13615..15243
                     /locus_tag="PC523_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2476060.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKVIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENVKRAVVIGGGFI
                     GLEIAENLSAMGIRVSVIDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIEG
                     ENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNKTLIADEYMLTNDKDI
                     YVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPEL
                     NAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQVM
                     GPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGEL
                     DSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGKN
                     SYMAQNRLKRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            15246..16193
                     /locus_tag="PC523_002551"
     CDS             15246..16193
                     /locus_tag="PC523_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDF2476061.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTIELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALAAEIHQEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEACPVKAAKLTDNGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQINRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGVDKFIEMLLSDEVLERKEDILDAPLHLTGGAVC"
     gene            complement(16470..16733)
                     /locus_tag="PC523_002552"
     CDS             complement(16470..16733)
                     /locus_tag="PC523_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HDF2476062.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQKNYD
                     YYGGFAKKNPCRMRVPNTGTWYIVVNQDGNSGIVNFSINTIQN"
     gene            complement(16832..17077)
                     /locus_tag="PC523_002553"
     CDS             complement(16832..17077)
                     /locus_tag="PC523_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476063.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTIYKDESSDSG
                     YYALLENEEERVVWDGEVVANNIFNKLWIVVNKVKTG"
BASE COUNT         5930 a         2595 c         2208 g         6573 t
ORIGIN      
        1 tgacagctaa gttactaata ataaagctta aaaatataaa aatcatttct tacttttagt
       61 ttacctataa taacaaaaga tagatttatg ttgtaataat taaaaatagt caaatatatt
      121 ttgatatggt taagtagaaa taatacttta gattaatgaa tattgataag ttataaaatt
      181 attttaaatt ttaaaaatca ctacttacta agataagtgg tatcatttaa cttgatactc
      241 cctattccaa ttgttttagg tattttacta attagaacct atttaatatt actttatgaa
      301 tagtgagtag ctgattaaat aataccattt atggtttgca gccatagatt aattaaattc
      361 ttaaagttct atcctaatat aattactctt cttctaaatt tttaaaatga ttttcaaata
      421 aatccaacat atctttatca acaacttcat catcatgtag tgctgaaatc aaactctgta
      481 aagaattgtc gtgtatatta cttaaaaaat cttttgtttc aacacttaaa tactccttat
      541 gtccaacaac tattgtataa tgagtgtatc tatctatttt ttctgcactt aaaaaatgtt
      601 ttttaacaag tcttgataaa agtgtcaatg tagttgtttg tttccaacca tatttttgtt
      661 ccatagcttc aatagtttct tttgatgtta ctgtatcatt tttttcccaa atgaacttca
      721 taacttttaa ttctgcttgt ggtatttttg aaattgtcat aaccttacgc ccctctcaaa
      781 cttttattaa attaatacta tcgcaatata gtttttaagt caaagatttt gtcgataata
      841 atatattcca attttttcca aataaatatt tcttatataa ttctataata aattacataa
      901 gaaatattca ttttacatca taaaattaaa aatataaata gtataattat ttagtcttat
      961 ctttaataaa caatccagta aatttataca tccaaagtgc attcataaac aacaatagac
     1021 ttgttgaaaa aaatactgtt ctaatgccta aatgtgctga tatttgtccc cctaaaaatg
     1081 ctcctgatga acagcctata tactgggcag atgcattata tccaaatatt ttccccacat
     1141 attcttctgg tgcaattttt taagcaagct gttcacagaa ggattaagcc ctgcaaccga
     1201 caaccctaat aaaaatctta acaacatcag ttgccatgca gtttttacaa aagcttgtgg
     1261 tatgaatata atcccagccc ataaaagtga aattaacaat actttttgag gtcctatctt
     1321 atctgacatt tttccaagtt tttgggctgc taataaattt gctaatcctg atgcagaaaa
     1381 tgtcaatcca gctattaaag ctacatttga tgcaaaattt gtgagctgtg atatatatat
     1441 ggttacaatt ggttctatag agtataaagc taattgagta acaaatgtag ttacaaaaat
     1501 agatgcaatt aaaaacttat ttggcaatat atctaattgt tttttaggtg tagaaattga
     1561 ttcttttatt tctttaggct taaaattatc tactacaaat aaaatagaga tgataaatga
     1621 tacaaacaac aatcctcctg ttataataaa tgtactttta agacctaaga ttgattctat
     1681 atatcctcct actgttggcc caattaagaa ccagtagttg atgcagttgc aagagtacct
     1741 aaagcccatc ctgaatgatt tttatctgtc tgtgttgcaa taagagtagt acatgcagta
     1801 gcataacctg taaaaactcc ttgaagtatt cttaatccta ataactgata aacattttga
     1861 acaaacgcca tggtaaatat aactattccc atcccaatac ttgcacgcaa aagcattgat
     1921 tttctcccat atttatcagc agctttccct caaattggtg aaaaaaatgc tgctactaca
     1981 aaagtacatc caaaaattat acctgaatac tgttgtatta aactaacatc agttaccccc
     2041 aattgtttaa tatataatgg aagtataggt gctatttggc tcatccctat agaactaaaa
     2101 aacataccta tccaacatac aaataaattt ctcttccaca attccatata gaattacacc
     2161 ccctgtattt taattttttc aataatacta taagtttatt aatttatgct attgattact
     2221 caatatattc atctcttctt ttataatatt gtttaggaaa ttcataacta gaaaataatg
     2281 gtatcgataa aaaataggag taaacatggt gcattttctt ctataaattt atagtcctca
     2341 tttaagtaca tattttttta cctaaaatat ttttaatata atcataaatt aaacaattat
     2401 aattctataa gatgatttta caataaattg ccatagttaa ctacacacat gtatgtaaaa
     2461 acatacctaa aatattttta attataaata catacagact tttatgacta aaataatttg
     2521 tttcaatttt attacaaata tacaaagtat attgttgtta acgtattccc atagtatcct
     2581 aaaactatat taagcactaa aaactatttt aaaatactgt ggaggtatca atatggaagt
     2641 atgcattctt atgggaagtc ctaagaaaaa aggcaatact gctactattt taaaaccttt
     2701 tattgaggaa ttagaaaact ataattcaaa tattgaagta atctggttat atgattgtaa
     2761 gatagagcca tgtattgctt gtaaaaaatg tcaggataac ttatttgatt ttggatgtta
     2821 ttgtaaagat gatgttcaga aaattttcaa gaaagttctt atgtgtgatt taattgtttt
     2881 tgctactcca atttattctt ggtactgcac ttctccaatg aaagcactac ttgatagatt
     2941 ggtgtatgga atgaataaat actatggtaa tgaaaaaggg cctgctctat ggtcaggaaa
     3001 accagtagca cttattacaa catgtggata taaacctgaa aatggtgctg atatttggga
     3061 aaatggtatg aggcgctatt gtaagcattc acagttaaaa tacataggaa tgcttgttga
     3121 acgtgattta ggtaaaccta attttatgaa taaagaaaaa gaagaacatt ctcgtcaatt
     3181 tgcaaaacaa atttattcta ttttagataa ttcaattaaa gtagataatt actaaatgtg
     3241 taatgccata agtaaagcat tgataatata aataaatcct acataattta cttatttaaa
     3301 ataaaatatg aagtctttaa aataaagtgt gtgttttcta aaaagtgaca ataattaaca
     3361 tgttttttat catttcaaca atgataaaga ggcttacctc tattttgaga tagcctcttt
     3421 attacttttt atatatcaat tattttcata gtaaccatca tcaaaaactc cttacatatt
     3481 acttctttca aatcctatta cttgttctta ctataaattt tttataaatt cggatatttc
     3541 ttcctttgtt gcatatgaac ttatcgcacc cttaatagtt gtactacgag ctgcataata
     3601 attagaaaac tctaaatact catttaacgt ttcttgtttt atttctttta tatcattcaa
     3661 tgttatttgt ttacacaata atgtgtataa aaatgaacct atataagaat cccctgctcc
     3721 tgttgtatct attgcattta ctttaatacc tttgcacata ccttttactt ttttagtata
     3781 ggcgtctact ccatcttttc cttttgtata taatactagt tcaacattcc ctacaaagag
     3841 tttatgtagt gatttttcta tatcattttc tcctgttata aattctaatt cttcatcaga
     3901 tatcttaaca atatgagcaa aaggtaaaaa ttctgatatt gcatttttac aagattgatc
     3961 actatcccat aaaggtaatc taatattagg gtcaaaactt attatactat tactttcaag
     4021 tgcatattct attgcttttt tatgtgctaa tttcattgga ctatctatta aatctactga
     4081 acaaaaatgt aggctatgac aattattaaa ccattctttt ttaacttcat cagcttctaa
     4141 aagcatatct gcacttggat ttctataaaa tgagaaatca cgattaccat cttcttttaa
     4201 tgatacaaaa gcaagtcctg tatttgcttt acttgttctc aatacataat cagtatttac
     4261 atttacttca tttaatacat ctattatata atcaccaaat gcatctttcc ctaacttaga
     4321 tataaaactt gattttcctc caagctttgc tacaacagca ctaacatttg ctggagctcc
     4381 tcctgctact cttctaaatc ctgatacatt ttttaattta caaccatttt gattaggtat
     4441 aaagtctatt aaagcttctc ctatactaat tacttttttc atcttttcta ccccttataa
     4501 tatgttttta taattcaata ccttgcttta atttgaattt agtatactta tagcatacta
     4561 ttttatcact ataaatcttt atttgattgg aattttcttt agcaaatatt ctactagtaa
     4621 atactgcttc tccattattt ataaaaattt ctaaaatact tctatctgct aatactttga
     4681 tgttagtatt ttcatcaata tttatatatt cttttctatt tgttccatac tcaacactga
     4741 aaactttttt aaagttgctt ctgtctaaag ttacagtgtt ttctacctta ttaaattcta
     4801 attttacacc ttcatcttgt gacgaaaata attccaatcc aaatttattg ctgtcaatat
     4861 tttttatatt aatattaagt tcatatacat tttcatcatt ttcgataata ttaatattct
     4921 tttttatagt attttgtcca cttatttcat catatcttaa tttaactaat tcatctacag
     4981 gcatttgtaa tagtttatta ttttttatat ttaattttct tggaacagtt agacaatgtg
     5041 cccatttatt tttatctgta gggtataaaa cttctcccat tccagcccat gcaaatagta
     5101 tagtttgtga gtcttttata aatgtttgtg gtgcataaaa atcaaatcct ttttccattt
     5161 ctttcattat atctatttca aatgttaaat tatctaaatc catattgcca atagcatata
     5221 ctacattata tatattttga taattaaatc ctttaggttc taatccttgt ggtgaaaata
     5281 ttaatatatc ttttccatct atattaatat aatctggaca ttcccacatg taaccaaaat
     5341 cttcatctat attttttaca tttaattcac ctttaaaatt ccaatctata gagtttttag
     5401 attcatatac aattatagct ccttttttat tacttgtttg agctccaagt agcataaagt
     5461 atttatcctt tcttttaaat acttttgggt ctcttacatg tcctgtatat ccttttggta
     5521 tttcacttac tattggatta ttttcatatt tagttatttt accatccttg tccattatag
     5581 ctaaattttg atatgcatat ctatcttctg cactatattt tatatttcct gtataataca
     5641 tatgcaattt tccatctact tctatcgaat tacctgaata tgctccatga gattcataac
     5701 tttcaattgg tattaatgct acatcttctc tattccaatt tacaaaatca tctgaactta
     5761 cataagccca atgcttcatc ccatgagtta catcatatgg ataccattga taaaatacat
     5821 ggtacttacc attataatat gctaatccat ttgggtcatt aagtagccca tgttgtggat
     5881 gtatatgaaa tattggttta tatttatcat ttaaagatat atttcttaaa tcatttaatt
     5941 cttcttttgt cgattctaat attgttctat atttaggctc tttgtacata tttttattcc
     6001 ttttctttta tagttttata taatcttaga agtcattagt atatatcact ttttatagtt
     6061 atttgattat ttataattta ttaattaact gattttttct gtaattttcc aagtaatatt
     6121 gttgttatca tagctacaac tattgttact aaatgtgcta atatatattg aacatgtcct
     6181 ccaccttgag tagacactat tgctaaccct ggtaccgctg ttgctccaaa tgccaatgct
     6241 gttacttttg tcatatatat gtatgcacca cctattgctc cacctataca ccctgctatt
     6301 aatggatatt tgtactttaa agtaactcca aatatggctg gttctgatat tccaaataat
     6361 actgaagcaa atgaagatat tcctatttga cgagctttta tatctttcca atttaatact
     6421 aagtatccta atatagctcc accttgacct attaacgcta ctgacataag aggcattata
     6481 aagttagttc ctgtatctgc tataagttgt gcttcaacag ctcctataac atggtgaagt
     6541 cctgttataa ctataacttg ctgtactcca gcaaatatca tatatccaaa tattcctaag
     6601 ttttcagtta tccataataa agaggatgtt actatatttg ctaagcctct tcccaatggg
     6661 ccaactataa caaatagtaa aaatgatgct actataactg tacatattgg tgctaagaat
     6721 aacttaagca tatcatgtat ttttgagtta aagaatttat ctagtttagc tactacaaac
     6781 cccatcataa gagcaactat tattcctcct tggaatccca ccaactcaac tttcaatcca
     6841 aataaatcta ttatttctgg tactacacta ccatttccta ctgagtatgc atttgtaagt
     6901 tctggatgaa gcattatagc tcctagtatc aaacctaata ctggacttcc tccaaatctc
     6961 atagttgaag aatatactac taacagtggc aatatagtaa atattcctgt tgatgctata
     7021 cttataaatc tattaagccc tgctaattga ggatacattt ctattattga caactctcca
     7081 aatattcccc tttgacctaa aagccctgta atacccatta acaatgctgc tgctaatagt
     7141 cctggtatta tctgcacaaa tacatctgat aaagatttga tagcttgttg gaatttgttt
     7201 tgtttttgag ttgattcaga tttaacatca cttaatgaca tattttcaaa tcctgatagt
     7261 ttactaaatt ctttataaac attatttact attcctgctc caaatactac ttgaagttga
     7321 ttagatgcta taaatactcc ttttactccc tctaattctt ctattgcctt tatatttgct
     7381 ttattattac tatctaaaac aattcttaat ctcgttgcac agtgtgctac accttttata
     7441 ttttgttcat ttcctatgta ctctaataat tgctttgata ctttgttata atcaattgtt
     7501 tttgcactca tctttatctc ctctcgattt aattaaaccg gtttcgaaac cggtttaatt
     7561 aaagactatc ataaatttat tttagatgca atatttcttt cgaaaaagtt ttcctaattt
     7621 ttggaattat agtttttcta ataaaaaaat acacattact aatatatgta atgtgtatct
     7681 taatttttat ttattgtact ttcaccttct attaattcaa atttaatctc ctttaatagt
     7741 ggttcttttc tttcttgtat taagtttact atcgtatttg ctgctacttt acctgcactt
     7801 tcattttcaa attttattgt tgtaagttta gggcttataa tagacaatac atcatatccc
     7861 ccaaatccag ctacagatat gtcttgtggt atatttttac ctctcttatt gatttccttc
     7921 attgttgcta ttgctatagt atcagttgca catattatca tatctggatt ttcattttct
     7981 agcatttctc cacttttttg tatagacgtt tcttggtcaa aatcagtata aaatactttt
     8041 gcatcatatc ctttatcttt taacccatta agtactccat tttttctatt tgaaccaact
     8101 gatatatcac tttcatctac gcctaaatat acaatgtttt tgtaaccttt agttgcaata
     8161 tattgcccca ttttatatcc tgctccataa tcatcattca ctatgctatt gataccatca
     8221 actttttgtc caactacaac tattggtata tctaactttt caatttcatt cttatgttct
     8281 ttagttattt cggtaccaac taatattatt ccatcaactt tcagtctagc taattttgat
     8341 atactatcta tttctgacct tatttttcta ctagtattaa taattagtga tgtatatttt
     8401 aattctttga gttcttcatc tattgccatc ataattttag attttacaaa agaatctaaa
     8461 cacggtgcta taataccaat aaagtatgtt tttttagctc tcaaactttg agcaaatgca
     8521 ttcggctcat atccatattc atctattatt gtctttattt tctctttagt agaatcactt
     8581 atatctttat tatttagata tctcgataca gtacttttag atactcctgc taatccagca
     8641 atatcattta ttgttatttt tttcataggt tatcaccttc tacatttgat atattttatt
     8701 tcattatatt atatatttaa aataaaatat agttatttat ctcttcaatg ctttttatac
     8761 attacttgta ctatctatac aacacttaga aaagtgtttt tttcactaag ttataatttt
     8821 tatttttgtt gttatattta attatatcta ctataaattg ataaatatct taaattattt
     8881 atcacataaa aaacttgtaa aaaaaaaata tatgaaaata aggctatgat tttcactgcc
     8941 atagccttat caataaatgc ttaatctcca tttaaaaaat aaattcttaa attataacat
     9001 agtattgcgg tcaaatattt attattctag aattatcagt aactttatat taattatttt
     9061 gccttattta tataaaatct atttttatat tattttttaa ataatagcat aactaaaata
     9121 gttttatcat ataatttgaa aatttcgact attgcaaaac ttttatcatc atcttaccac
     9181 catcaaatat gtattcaacc atattatttt catataaata agttatataa gaacgtattg
     9241 tactcccaac taatacatat tgattagcat caagcttgac attgtacaaa tcacacatct
     9301 tttcaataac tttatcaatc ataactggct cacaacaaac ctttttaata ttatctatta
     9361 tttcttccat cttcttctta ttaatatcaa tcagattttt tatatctgta gttttgacag
     9421 agtgacttgg aacaaagaag tctgcctcta acattctcaa tttttctagt gtcttaaact
     9481 gtgaatctat atccaataga aagaaaaaat gatatttagt aattatattc tctggtgtaa
     9541 gactatctgc aacaaacaaa acattatctg gagttttaac accaatcatt tcaaaataat
     9601 gacctggaag agaaatcgct tcaagttctg tatctacaat tttgccattt gaaggtataa
     9661 tataatcaac ttcactttcc tttgctaata aaaatttatt cttcagtgct ttacttggat
     9721 aaccaccata taaaaatgct gattctaata ttggattttc tgtaaaatat ccttcaattt
     9781 tagttgtggc aatcttgcat tttgtttgat tcttcagata tgcatttcct cctatatggt
     9841 ctgcatttga atgagtgttt attattaatt taggaatcaa attatgttct tctaataatc
     9901 ttaaaacttg tctagctgta tccttattgt ttccactatc aattaaaatt gcatctttac
     9961 catctacata tacacctact attgtaggga gttgaatata atatgtattt ccttttatat
    10021 gttctagagt taatttagcc attttataac ctccctttaa atttactttt aattatagca
    10081 ttaattttct gaaaattcca atcaaattat ttttataata tatttttatt tcttttatta
    10141 aaaattggaa aaactatata atatatatta taatctatat ataaggagtg atgttattga
    10201 aaaaagatag aataactttt tcagaaattt taatattaat tgtaggctgt atattaatgg
    10261 caatttctct taacttattt tttaacccac atgcaatcgc tgctggtggt ataacagggc
    10321 ttggagttgt tttaaattca ctattcggtg tagaactttg gattgtcaat ttacttttaa
    10381 atgttccatt attcatattt gcttacaaaa tactttcaaa gaaagattgt tttaaaacag
    10441 ttttaggaat aatttttctt accatagcac ttaagcttac tgcgaatatg gctacattag
    10501 atataacaaa cgatatgtat cttgcaatta tatctggttc tattttaatg ggagttggtc
    10561 aaggtcttat ttttagaata aatggctcta caggtggaac tgatttaatg gcactcctac
    10621 taaataaata ttttcctaca tttagtatac ctgttttaat gggtattgtt gactgtgttg
    10681 tagttgtttt atcaggtata gtaaatagac aggttgaaat tgcactctat tcaactgtgg
    10741 ctttatatat tttagtaaaa gttagtgatt tacttattga aggatttaat tattccaaat
    10801 cattcactat tatatctgac ctttcaaaag atataagtaa aaaaataatg gaagatttag
    10861 atagaggtgc aactattcta aaaggtgaag gtgcatatac tggagaaaat aaaaatgtac
    10921 ttttagtagt agttgaaaag aaagaagttg tcgaattaaa aaaattagta aaaaatgtcg
    10981 accctgatgc attcattata attacagata tacatgaggc tttaggtaac ggatttaaaa
    11041 aaatagaata attaacagac aaatagcaag tataattaca tatataaatg caacttttta
    11101 tatgtcgtat tacacttgct atttatatat tataaaacta caaaacaatt taaaccatat
    11161 agtggcaata tgtttaaatt ataaaatatt catgtagtta tataataaat aatttataaa
    11221 tatagccaaa ttggtctaaa tacatatata atatagctaa tatacttctt atacttgata
    11281 ccatcaataa aactaaaaat ataaaaagga gaacttaaaa tgagtgttag tttacaaaaa
    11341 ggacaaagag ttagcttagt aaaagacaat aatcctgtaa aaaatctaat tgtaggatta
    11401 ggatgggata tgaataaatt gggcaaaaaa aattacgacc tagatgcatt tgcaatagca
    11461 ttgactaacc aagataaaat gaacgctaga ggtgacttag tttattttgg aaatcttaaa
    11521 cacccttcca aagctattat acacactgga gataacttaa ctggaaaagg cgaaggtgat
    11581 gatgaacaaa taattgttaa tttagaaaac atacctgaat atgtacataa aatagttttt
    11641 ggagtttcta tatacaaagc taaaaaaaga gaccaggatt ttggtcaaat gaacaactct
    11701 tttataagac taattgattc aaattcaaaa caagaattat ttaaatacaa tcttcaagaa
    11761 gacttttctg gtaaagtaac agttttggca ggagaaattt atcgtagaaa tgaagaatgg
    11821 aaatttaatg ctttaggaat tggtcaaaat gaagagcttc gtgaattaat taatacatat
    11881 aagtaataat tacaataaaa atttacaatg aggagttgat ttaaatgggt ataactttag
    11941 caaaaggaca aaaagtaagc ttaacaaaaa gtaaccctgg cctaaaaaaa gtaatagtag
    12001 gactaggatg ggatattaat aaatatgatg gtggttttga tttcgattta gatgcttctg
    12061 catttttaac tggaactaat ggaaaagtaa caaatgatgg agattttata ttctataata
    12121 atttaaaaca tgtttctgga gctgttgaat atatgggtga taatagaaca ggtgttggtg
    12181 atggtgatga tgaacaaata aatgtagact tatctaagat accccaaaat attgctaaaa
    12241 tatcatttag tgttactata aatgaagcaa ttacaagaag gcaaaatttc ggacaagttg
    12301 aaaattcata tataagaata tacaacgaag aaactaatga ggaacttatt aaatatgaat
    12361 tgggtgaaga ttttagcata gaaacagcaa tagttgtagc agaactttat agacataacg
    12421 gagagtggaa atttaatgct ttaggttctg gatttgaaga tgggcttgca ggattatgta
    12481 aaaattttgg agttaatata ggctaatgta atatatctaa ataaaaataa gagtatatta
    12541 atatactctt atttttattt agatatatta taaactgttt acactcctac tcttctttct
    12601 agcttgtttt tttccatatt ttactgaaat atcaaagaat gctgaaatat tttctgtaaa
    12661 aaacttactt ctatgatatg caatatcaaa tgttctatgt aaattaatac cttcaatatc
    12721 acaagcccac aaaagatgct tttttaaata atcctcaata agtaactcag atataactgc
    12781 aatgccaaaa ttatctacaa ctgctcttaa tatagcttca gagttataac atgaccatct
    12841 aatattcatt gggattttta attcttttaa ttgtttttct aattgtgctc ttgtaccact
    12901 tcctaattca cgcataatta gtggttgatt tgaaagttct gaaactttta tagagtctct
    12961 tttgtagaac tcatgcttat gtgaacatat aagtacgaga ttatcattaa taatgctttt
    13021 tgtaacaata tccgggtgct taatttttcc ttctacaatt gcaatatcta attcactatt
    13081 aagtaatttt tttgcaatca atcttgtatc ttctacaaat acttcaggtt ctataagagg
    13141 atttaccttg tataattcta acattattgg gcttattaga catgtaccaa cagttactgt
    13201 tgcccctact cttatacgga tattttttgc acaatgcttc atacaatcat tcatttcttc
    13261 atttacagct aaaagatgcc tcccatatcc tagaagtttt tttcctgcct cagttaaata
    13321 taattttttt gaaattctat caaataactt aacaccatac tccttttcta tttctgaaat
    13381 agcttggcta actgatgact gggtaatata catatttctt gctacttcac tcattttacc
    13441 acattcagca accttaacaa atatctcaag actacgaatt gtcatattat cacaacctta
    13501 gcaaacatta gtaaatgctt atgattttat taatatttta gtattttaca aatacattgt
    13561 caagaagtat aattttatta tagagttata aagtagattg gagtgtgata tattgtgaaa
    13621 gtattaatta taggtggagt tgctgctgga acaaaaactg ctgcaaaaat aaaacgtgaa
    13681 atgggtgata attgttctgt tacaatacta aataaaggtg atgatatttc ttatgcaggt
    13741 tgtggtcttc catattacgt tggtaaggtc attgaagata aatcatcttt aatcgtaaat
    13801 acgcctgaaa gtttttctaa actaacagga gcagaagttc gttgtggagt tgaagttaca
    13861 tctattgata gaaatgcaaa acaagtaact ttgaaaacgc tttcaacagg agaaaaagaa
    13921 aatctaagct atgataaact tgtaattgct acaggggcgg atccaataaa accaccaata
    13981 cctggaatag atttacctgg agtattcttt atgcgtactc caaatgatgc tataaaacta
    14041 agagattcta ttgaaaatgt taaaagagct gttgttattg gtggaggttt cattggtctt
    14101 gaaattgctg aaaacctttc tgcaatgggt atacgtgtaa gtgttattga tatggctgaa
    14161 catgttatgc ctggatttga tactgatttt gctgaatatg ttgaaaatca tatggctgat
    14221 aaaggcataa tgatttttac aggtgaccag gttgtgggta ttgaaggtga aaataaagtt
    14281 gaaaaacttc gtactaaaaa tagagctatt aaaacagatt tagtagttat gtcagttgga
    14341 atccgtccta acacaggttt tctaaaagac acaggtttag aatttgctcc taataagaca
    14401 cttattgcag atgaatatat gcttacaaat gataaagata tatacgtagt tggagattgt
    14461 gcttttgtaa aaaattcttt aacaaataaa cctacatggt cacctatggg gtcatctgca
    14521 aatcatgaag gtagaatttg cgcacaaaat atatctggaa aatctaaaac ttacaatggt
    14581 gttcttggaa caacagtagt aaaattacct gagctcaatg caggaaaaac aggtctaagt
    14641 aaagaaatag ctgaaaaaga gggttataat gtttgctctg taaccatagc tactgatgat
    14701 aaagctcact attatccagg tgcatctaat tttattataa gaatggttgc tgaaaaaaat
    14761 actagaaaat tattaggtgt tcaagttatg ggacctggag cggttgataa aatagtagat
    14821 atcgctgcta ctgcaataac tttgggagct gacctttatt cattagaaag tatggacctt
    14881 gcatacgcac cacctttttc aactgctatt cacccttttg tagtggcagt aaatgtttta
    14941 caaaataaac ttaatggaga acttgatagt gttttacttg acgaaattga agattttgac
    15001 agttggacta aacttgatgt ttcaaaagct ccttctattc ctagtttgcg ttatattcct
    15061 gttggtgaaa ttaacggaga aattgaaggt cttgctaagg atgaaaaaat tcttcttata
    15121 tgtgctaaag gtaaaaatag ctacatggcg caaaatcgct taaaacgctt tggctataca
    15181 aatacgaaag ttcttgaagg tggaatacta ttttatccaa atcttaaggt aatggaggag
    15241 taaatatgag taatgttaca gcagaacaaa aaaaggaagt aaaaggtaga ggttttctac
    15301 caagcagaga tggagaacat tttgcagcac gtataataac tgtaaatggt gtaataaatg
    15361 catcacaagc aaaaaaaatt gcagaagctg ctgaaaaatt cggaaatggt caattagcat
    15421 ttactactag actaacaata gaattgcctg gtgtaaaatt tgaagatatt gaagctttaa
    15481 gtgaatttat cgctagcgaa aatttaataa caggtggaac tggctcaagg gttcgtcctg
    15541 ttgtagcttg taaaggtact gtttgtgttc atggtctagc tgatacacag gcacttgctg
    15601 ctgaaataca ccaagaattt tacaaaaatt ggtacgatgt aaaactacct cataaattta
    15661 aaattggtgt tggtggatgc cctaataatt gcattaaacc tgatttaaac gatcttggaa
    15721 ttgttggtca acgtgtacct gattatgact cagaactttg tgttggttgt aaaaaatgtg
    15781 ctgttgtaga agcctgtcct gttaaagcag ctaaattaac agataatggt aaacttgaaa
    15841 tagatagtaa tctatgtaat aactgtggta agtgtataga aagttgtaat tttgatagta
    15901 ttgaagaaaa agaatctggt tataaagtct atataggagg gaaatgggga aaatcagttc
    15961 gtcctggaac acaaattaat agattatttt caaaagaaga acttatgact ttaattgaaa
    16021 aagcaattct tttatttaga gagcagggta acacaggtga acgttttgga ataactatcg
    16081 atagaatcgg tgttgataaa tttatagaaa tgcttttatc agatgaagtt ttggaaagaa
    16141 aagaagacat acttgatgct cctttacacc ttacaggtgg agctgtatgc taacaataaa
    16201 tattttagtt gtataaatat cattaacata ttgaaaaaat tttagttaag aataatcaaa
    16261 attcttatga taaatgaggc tatctcaaaa cacagataag cctctttttc attgttgaaa
    16321 tgataaagta tatttgagta taaaaaagag tgtctcataa actataaaat tcattttgag
    16381 gcacccaaat tattttataa taattgctta ttaattttat tttaaatata taaaatgcac
    16441 aaaaagtaag aatatcttat attctaaaat taattttgaa ttgtattgat tgaaaagttt
    16501 actattccac tatttccatc ttgatttact actatatacc atgttcctgt attaggaact
    16561 ctcattctac atggattttt ctttgcaaat ccaccatagt aatcataatt tttttgattt
    16621 ttaaatttca cataatttat atggtctaac aatattacat tggcttgttt ctctaaattt
    16681 acctctacaa tatccccttt ttttaggttc atcttactat aactatattc cattgaatta
    16741 ctcctccagt atcgaataat ctttgttatt tactcatctt aaatatgtaa aaattcattt
    16801 aaaatattac aaatactata caattggact attatccagt tttaacctta tttactacaa
    16861 tccaaagttt attaaaaata ttattagcaa cgacttcccc atcccataca actctttctt
    16921 cttcattttc taataaagca tagtacccag agtcagaact ttcatcttta tagattgtaa
    16981 cataaaactc ttcatcacta tattcaggta tatttttaaa gtcttcagat attttacatg
    17041 ttaatatata tccttctagt tcttcatcta taaacaaagc aattcctcca atctctatat
    17101 ttttactaat tataccatat tatgtcataa actttttatt tcgttgtaaa attctatttt
    17161 ttctttactt tataaacaat ttatatattt ttttagtttt ttactttaca ttacactaat
    17221 taattctttt gttttcttat atattccttt ataattcata aatttaacca ttagtttata
    17281 cgactttata tacgtaaaca tataat
//
LOCUS       DAMJVM010000071        17073 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.074, whole genome shotgun sequence.
ACCESSION   DAMJVM010000071 DAMJVM010000000
VERSION     DAMJVM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17073
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.074"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            <1..859
                     /locus_tag="PC523_002554"
     CDS             <1..859
                     /locus_tag="PC523_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HDF2476064.1"
                     /translation="NAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNV
                     GANYLREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVA
                     QGAAMMTETEMEYKVTDGIYDYIPNQCLTDLVYNNMVFVGCPKTTPEEEEFAKKLCDT
                     LTREERLGVATSFQNDKSIVESYIHKGIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMA
                     AWPVGIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSL
                     DGQKFKPLV"
     gene            894..1118
                     /locus_tag="PC523_002555"
     CDS             894..1118
                     /locus_tag="PC523_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HDF2476065.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(1441..1839)
                     /locus_tag="PC523_002556"
     CDS             complement(1441..1839)
                     /locus_tag="PC523_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HDF2476066.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKNDEE
                     KKKFFHKFSIVVWTIWLIPYFIGMFIGMAG"
     gene            complement(1842..2243)
                     /locus_tag="PC523_002557"
     CDS             complement(1842..2243)
                     /locus_tag="PC523_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2476067.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDEKELSDLVKNLRTILAKVKER"
     gene            2604..3590
                     /locus_tag="PC523_002558"
     CDS             2604..3590
                     /locus_tag="PC523_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2476068.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYNQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRVICKLGTITNCIPSFWVALVLILVFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLVLSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEAAI"
     gene            3574..4485
                     /locus_tag="PC523_002559"
     CDS             3574..4485
                     /locus_tag="PC523_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2476069.1"
                     /translation="MKPQYSDFEIVGENYILVAKDEHVETKKNLFRKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASEFVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIRNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            4598..6166
                     /locus_tag="PC523_002560"
     CDS             4598..6166
                     /locus_tag="PC523_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2476070.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADKPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGILPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQVTPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNHTIDKI
                     LQKARETENKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            6241..7266
                     /locus_tag="PC523_002561"
     CDS             6241..7266
                     /locus_tag="PC523_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2476071.1"
                     /translation="MEHLLEVNNLSVNFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSNLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLHPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPKIDVPVRVNC
                     GRVISNE"
     gene            7259..8203
                     /locus_tag="PC523_002562"
     CDS             7259..8203
                     /locus_tag="PC523_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2476072.1"
                     /translation="MNREKMLEIKNLKQYFHLDKNTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIVRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGIYKNKTDRMNKVYEMLNLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRTRIEYNSN
                     VYDCSNEKSLSWIEVSDGHFVYSSKADINKYQQNLKVV"
     gene            8563..11220
                     /locus_tag="PC523_002563"
     CDS             8563..11220
                     /locus_tag="PC523_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2476073.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQNELLGIKKGTTVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAVLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERIELIKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLKYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHRIEYFVFTSLG
                     EEEVINYNDINIINAVNTNINKSVEMNITDASLFNNVKFKNMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGNCNAISFDL
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVMVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKYYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMNRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVALINEQRINIRGIDY
                     ISKKDIDKDKSINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKMLNYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            11434..12063
                     /locus_tag="PC523_002564"
     CDS             11434..12063
                     /locus_tag="PC523_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476074.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAIIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEIMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEQYNSEEVIDTAFRFNHWQY"
     gene            12181..12507
                     /locus_tag="PC523_002565"
     CDS             12181..12507
                     /locus_tag="PC523_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2476075.1"
                     /translation="MNILLVCANGASTGVLVEKMKNFCSEHEKLKTKTINIEATSFEN
                     LKSYIQANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            12523..13812
                     /locus_tag="PC523_002566"
     CDS             12523..13812
                     /locus_tag="PC523_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2476076.1"
                     /translation="MEALQNNLRKFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVIAQLVVCGQVIDGALDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVLGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSLNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATALDDDSFDF"
     gene            13836..14165
                     /locus_tag="PC523_002567"
     CDS             13836..14165
                     /locus_tag="PC523_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2476077.1"
                     /translation="MVPEIEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            14313..15641
                     /locus_tag="PC523_002568"
     CDS             14313..15641
                     /locus_tag="PC523_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2476078.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKNPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLESRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TKERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGEIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPELN"
     gene            16059..16919
                     /locus_tag="PC523_002569"
     CDS             16059..16919
                     /locus_tag="PC523_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HDF2476079.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCN
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
BASE COUNT         6794 a         1884 c         2993 g         5402 t
ORIGIN      
        1 aaatgcacct tggagaggtg gaagtctagc aaacttatct gtaaaattta aatttaaagg
       61 tataacagcc catgcagctc aagctcctca taatggtaga agcgcacttg atgctgttga
      121 aattatgaat gttggagcta attatttgag agagcatgtt attgactcta taagaatgca
      181 ctatgttata actaatggtg gtggaagacc taatgtagtt cctggctttg ctgaaagttg
      241 gtactttatt agaggaaaaa aagcaaagga cgcagaacac gttcttgata gattaataaa
      301 agtagctcaa ggagcagcaa tgatgacaga aactgaaatg gagtataagg ttacagatgg
      361 aatatatgat tatataccaa accaatgttt gactgatttg gtttataata atatggtttt
      421 tgttggatgt cctaaaacta ctcctgaaga agaagaattt gcaaaaaaat tatgtgatac
      481 tcttactaga gaagaaagat taggtgtagc aacatcattc caaaatgata aatccattgt
      541 agaaagctat atacacaaag gtatagttga tggagacaaa gataaaggac tagctggttc
      601 tactgatgtt ggagatgtaa gttatgttat accagtggct caatttgcaa tggcagcatg
      661 gcctgttgga attgcaagtc atacatggca gtcatgttca tcagcaggtt caaatatagg
      721 attttctgct atgataaatt cagctaaagt attggcttgt agtgcctatg atgtatttat
      781 ggatactaaa attattgatg aagcaaaaat cgaatttgat aaatcattag atggacaaaa
      841 atttaaacca ttggtataat tttggtaata aaatatttct ggggggaata gctatgagag
      901 aaaaagttga aaaagtgctt gaagaaaaga taaaaccagt attgcaaaga gatggtggag
      961 atgtagagct tatagatgta aatgaaaatg gtgtagtttt agttagacta caaggagcat
     1021 gtagtgggtg tccaggagcg actatgacaa tcaaagctat tattgaaaat gttttggtga
     1081 gtgaagttcc aggagtaact caagtattag gtgtttaaca gttagagttt attaaatata
     1141 tgttatacat aaatatttaa taaagaagta tgaaaaaaat agtgaaacgt tatatataaa
     1201 aatatgaaaa agataataaa acattataga aacaattaaa tattccatat agatatgaaa
     1261 aattgtgaga aaataataaa atattatagg aataattaaa tattctatat taggtatgaa
     1321 gaaatatgaa agacataatg aagtattata gataaatatg taaaagtgtg aaagaaaggt
     1381 gtagataaat ttgttttata caaatttacc taccatattt cttacacact ttttattttg
     1441 ctatccagcc ataccaatga acatacctat aaagtatgga attaaccata tagtccatac
     1501 aactatacta aacttatgaa agaatttttt cttctcctca tcatttttgt ataggacaaa
     1561 agtagcccaa cttgcatgaa aaagcataag cacaattgca agaagacctg taatactatg
     1621 aaggttttgt gataaagcgg attttacttc aaaagtatgg ctgttggcta tgttactcat
     1681 tgtaaatgtt cctaaagtgt caaatataaa accaagccaa aatataatta catgtttctt
     1741 tttcaaaatt ttagcctttc tttctccaaa tacacctata gtgtagaaaa ttaaagctga
     1801 tgttataaat actattgcaa gtattaattt ataattcata attatctctc ctttactttt
     1861 gccaatatag ttctaagatt ttttactaag tcactcaatt ctttttcatc acaattttta
     1921 aaaatatcat caaaactttc ttgcatctta attttaaaat ttgttgcaaa ttcaaatcct
     1981 gttgttgtaa acttaacgta tgtatttctt ttatcattag atttcttgaa tttttctata
     2041 tatccaatct gttctaatct gctgattatg cctgaaaccg tacctttagc caaagacatt
     2101 tcatcacaaa gttgtgatat tgtaagttcc tgattatgag ctattagctt tataactata
     2161 atctgttggt gtgttaatcc attttcttta aaattttctt ccactgtgta catagtttta
     2221 gaataaagtt cttttaagag catggctatt ctaaaacagt cataattagt tttcaataaa
     2281 atcacctctt aaatggtttg catacaaact aaatataata tacttttatt atatagtcaa
     2341 cctaaaattt tataatttca tataaaataa tgaagaatag aacataaaaa ttttatttta
     2401 gatataaata aaataatttg atgaaatttt aacaattttt aaaaagtttg tttacacagt
     2461 taataaatga tgctaaaatt aactcataga taaatattga aaatttaaat ttgtttaatg
     2521 ataatagaac cacgaaggtt ttaacgttac tttacgtttg cttttatgtg gtttttttta
     2581 ttgcaacaaa ggggggttgg ggtttgttaa aggatatact gaaaaagttg ttgcagttta
     2641 ttttagtaat gtttttatta tcttttattg ttttttacat ggcaagactt gctccaggag
     2701 acccattaat ctcttattat ggggatggag tagaaagaat gagtacacaa gaaaaagaaa
     2761 atgctatgaa aaaacttggt ttagatgaac caatatacaa tcagtatata aagtggattt
     2821 cgaatgcatc aaaaggagaa tttggaattt catttaaata taaacaaaat gttacttctg
     2881 ttataaatga tgtctatata aacactataa tattaggtgg ttcaggatat atacttacct
     2941 ttgtactagc cttattgtta ggaatatttt gtacactaca tgaagataag ctcattgata
     3001 gagtcatatg taaattagga acaataacca actgtattcc atcattttgg gttgctttag
     3061 tactcatact tgtatttagt ataaacttaa gtatacttcc aagtagtggc gcttattcaa
     3121 tgggagaaga atccagtata ttaagtagaa tatcacattt aattttacct cttacagtac
     3181 ttgtattgag ccatttatgg tattacacat atatgataag aaataaatta ctagaagaaa
     3241 taagagaaga ttatgtactt ctatgtaaag caaaggggtt aaataataga actattgttt
     3301 ttaagcattg cctgagaaat ataatgccat catatatcag tataatggct atttcgatac
     3361 ctcatatact aggcggaact tatgtagttg aaaaagtttt ttcttatcct gggctaggaa
     3421 cactttgttt tgaaagtgca aagtatcatg attataatat gttattagtt ttatgcttaa
     3481 taacaggggc tttagtagta tttgggaata tgttagctca aattataaac aataaaatag
     3541 accctagaat gaaatatgat agaggtgata ctaatgaagc cgcaatatag tgattttgaa
     3601 attgtaggag aaaattatat tttagtagca aaagatgaac acgtagaaac aaaaaaaaac
     3661 ttatttagaa aaattaaaga attaccatat atatcaatta ttattttatc aattatagta
     3721 attggaagtg tattctcatc ttttataatg acacatgagc cgacatatat ggatttagca
     3781 agctctaatt tagctccaaa taaaactttt ttatttggaa cagactcaat gggtagagat
     3841 atatattcta tgatatggta tggaggaaaa atatctttat ttataggatt attttccact
     3901 gtaatatcaa ctgttatagg aattgtttat ggtagtataa gtggttcagc atctgaattt
     3961 gtagatgatg caatgatgag atttacagaa atcatactta gtataccttc aatattaatt
     4021 ataatatttg tacaagctat acttggaaat tcaaatccag tatctatgtc tatagtaata
     4081 ggtataacaa gttggatgaa tatatctaag atagtgagaa cagaggttag acaaataaga
     4141 aatagtgaat acatactagc agctaagagt atgggaggag gattcttcta tgtattaaag
     4201 caacatttac ttccaaattt tgtggcatca ataatgttca tggttgtaac aaatataggt
     4261 gcagctattg gtactgaatc aacacttagt tttttaggaa ttggtttacc aatagaaatt
     4321 gtttcttggg gaagtatgtt atctttatca gaggaagcat tactttcaaa cagatggtgg
     4381 attatactga ttccgggtat atttttggtt accacattgg tgtgtataac aaatataggt
     4441 aattacataa gaaagaataa taataaaaaa tctagtaatt tataaatcag catttgagta
     4501 taaaataagc aaatactgag gtatttgaaa tatctattta aataaatggg gtcaaagctt
     4561 tgaaaaatat tttataaaaa gcaaaagggg gagcatcatg aaattaaaga agttaaaagt
     4621 tttaagttta gtaatgatac ttagtctaat ggcaggttgt tctagtggag gagacaaaga
     4681 taagaaagca gataagccta aagatggaaa ggtacttgtt tatggaagta atgactatac
     4741 aagtataaat cctgcactat atgaacatgg agaaataaat tcattaatat ttaatggatt
     4801 gacagctcat gatgaaaaca ataaggtggt tccttgtcta gcaaaagatt ggaaatttga
     4861 tgaggcaaca aatacatata cttttaattt aagagatgat gttaagtggc atgatggaga
     4921 gaaatttaca gcaaatgatg ttaagtttac aatagaaact ataatgaatc cagacaatgc
     4981 ttctgagata gcatcaaatt atgaagatat aacaaaaatt gatgtagtta atgataacac
     5041 tataaaaatt actttaaaag ctccaaatac agcaatgctt gattatttaa cagtaggaat
     5101 attaccaaaa catgctttag aagggaagga tatagctact gatgaattta atcaaaagcc
     5161 aataggcact ggtccattta aacttgaaaa atgggacaaa ggtcaaagta taacacttgt
     5221 taagaatagt gattattttg taaaagaacc aggattagat aaagtagtct ttaagattgt
     5281 tccagatgat aaggctaagg caatgcaatt aaaatcaggg gaattagact tagctcaagt
     5341 aacacctaaa gatatgtcta actttgaaaa agacgaaaag aattttaaag taaatataat
     5401 gaaaacagcc gattatagag gtatattata caattttaat tctaaattct ttaaagataa
     5461 aaaagctaaa ggattgccaa atgctttaag ttatgcaata gatagaaaag caatcgttga
     5521 tagcgtatta ttaggacatg gtgttccagc atattcacct ttacaaatgg gaccatataa
     5581 taatcctgat atagaaaagt ttgaatataa tccagaaaaa gctaaacaag aaattgaaaa
     5641 gctaggttgg aaattaggtt ctgatggaat atatgaaaaa gaaggtacta agttagcttt
     5701 tgagataaca gcaggtgaga gtgaccaagt tagagttgat atggctaaga tatgtgcaca
     5761 acagttaaaa gaaattggtg ttgatgctaa agctgtagtt gtaactgaaa cagactgggc
     5821 taatcaagat gctcatttaa taggttgggg aagtccattt gacccagatg accatacata
     5881 taaagtattt ggaacagata aaggtgcaaa ttacagtgct tactctaatc atactataga
     5941 taaaattctt caaaaagcaa gagaaacaga aaataaagat gaaaagttaa agttatataa
     6001 acaattccaa gtagaaatga ctaaggatat gccttacaca tttattgctt atatagatgc
     6061 aatatatgtt ggaaaaccaa atatcaaagg attaacacct gatacagttt taggtcatca
     6121 tggtgtaggt atattctgga atatagctga ctggacaatt gaatagtatt gaaattaatt
     6181 agaaacctta gggataaaag tatatcccta aggtttgaat agcatagggg ttgatggttt
     6241 ttggaacatt tattggaggt taataatcta tctgtaaatt ttaaagtaga agaaggagaa
     6301 gttcaggctg taagaaacgt gtcttttaat ctgaaaaagg gtgagacatt agctatagtt
     6361 ggagaatctg gttgtgggaa atctgtacta tgcaagagtt tgatgaggat actcccatac
     6421 aatggatata ttaaaaatgg agaggtttta cttaaatcaa gtaatttggt taagaagtct
     6481 gaaaaagaga tggaagatat aagagggaaa aatatatcaa tgatatttca agaccctatg
     6541 acatctctca atccaactat atcaatagga aagcaaattg cagaagctgt aatcattcat
     6601 caaggtataa gtaaaagtga agcaaaaaag agggccatag agcttattga attggttgga
     6661 atagataatc ctgagaaaag atttaaacaa tttccacatc atttttcagg tggaatgaga
     6721 caacgtatag taatagctat agcacttgct tgtaatccag atgtattgat tgctgatgaa
     6781 cctacaaccg ctttagatgt aactatacaa gctcaaataa ttgatttaat aaaggattta
     6841 caacataaaa taggtttgtc aataatattt ataactcatg atttaggagt agttgcaact
     6901 attgctgata gaatagcagt tatgtatgca ggaaagattg tggaaatagg aactgtggag
     6961 gatatatttt atgaccctag acatccatat acttgggggc tgttaggttc attacctact
     7021 ttagattcgc aggatgacta tttgtataat atacctggaa tgcctccaaa cttattgcac
     7081 cctccaaaag gagatgcttt tgcaataagg aataaaaatg cacttaaaat agattatgaa
     7141 aaagagccgc caatgtttaa aatcaatgat acacatagtg cagcaacttg gttattacat
     7201 ccagatgcac ctaaaataga tgtaccagtt agagtaaatt gtggaagggt gatttctaat
     7261 gaatagagaa aaaatgctgg aaataaaaaa cttaaagcaa tattttcatt tggataaaaa
     7321 tactactgta aaagcagttg atgatataag ttttgatata tataagggtg aaatatttgg
     7381 attggtagga gagtcgggtt caggcaaatc tacaactggt aagactatag taagactaca
     7441 tgaatctact ggtggagaag taatttacaa aggaaattgt atatccgata agaagacata
     7501 caaatttata aaaaaagatg taaacaaaag tatgcagatt atttttcaag attctacttc
     7561 atcattaaat ccacgtatga caattgcaga tataatttca gagccactaa aaattcaagg
     7621 tatctataaa aataaaacag atagaatgaa taaagtatat gagatgctaa atctagtagg
     7681 acttgataga agttatgcaa ataagtatcc atctgatttt tcgggtggac aaagacagcg
     7741 tattggcata gcacgagctc tttcagtaga cccagaattt ataatagctg atgaacctat
     7801 agcttctttg gatgtgtcta tacaagcaca aatagtaaat ttatttaaga agttacaaca
     7861 agaaaagaat ttgacttgtt tatttatagc ccatgattta tctatggtga gacatattag
     7921 tgaccgtata ggagttatgt ataatggaaa attagtagaa ctggcagatt caaatgaatt
     7981 gtataataat cccattcatc catatacaaa atcactactt tctgcaattc ctgttcctga
     8041 ccctagatat gctaagtcaa gaactagaat agagtataat tctaatgtgt atgattgctc
     8101 aaatgaaaag tcactaagtt ggattgaggt ttcagatgga cattttgtat attcttctaa
     8161 agctgacata aataaatatc aacaaaattt aaaagttgtt tagtataaaa aaacttgaac
     8221 atgtatttat ttgaaataca aactaaaacc ctatttttgg ataaatttta aaactactcc
     8281 aagatagggt tttttattta cacttttcaa atataataaa ttcaaatata ataaaattaa
     8341 aaacaagtaa agtatattgt taagaaagta ccataaaatt aaaaaatagt attttagaaa
     8401 aaattatcaa taaccttatt ttttctacaa taattttata taatttaaaa taagacaagt
     8461 ttgataaatt ataatcttag ttaaagaata agagtttatt aaaaaacaaa gtgagtataa
     8521 ggattttagt taacaaaata ttataatgta ggggagcttg taatgatata taaaaaagat
     8581 aaagtaatga aagttttaga atcatcatat agggaaaatg aaaaagggat tacagctaac
     8641 caattatcag aaaagacagg aattgcaaga aacaatataa gtacatattt aaaccagcta
     8701 ttcaatgagg gtatggttac aaagataaaa ggaagacctg tgtattatat ccctgcaaat
     8761 cttaacctta acaagaatga attgcttgat acacaaaatg agttgcttgg cataaaaaaa
     8821 ggaacaacag tagaaaacaa gataaaaaaa gatagttttg aaacactaat aggtaaagaa
     8881 catagtttaa gacctataat agaaaaatgt aaggttgcag tgctttatcc tacaaatgga
     8941 cttcatacca ttatctatgg agaaacagga gtaggaaaga gtatgattgc tagatatatg
     9001 tatgactatt ctatagatag tggaattagg aaaaataaag ctcctttcgt aacatttaat
     9061 tgtgctgatt atgctaataa tgcacaatta cttatgggac atatctttgg agtagaacaa
     9121 ggtgcttata caggtgcaga atcttccaga aaaggacttt tggaaattgc aaatgggggt
     9181 atattatttt tagatgaaat tcatagatta ccagcagaag gtcaagaaat gttgtttact
     9241 tttatagata aaggaacatt taaaagacta ggagatgcaa cgaaagaaag agagtctaag
     9301 gtattaataa tatgtgctac cacagaaaat ccgacatcaa cactattaga tacattcaat
     9361 agaagaatac caatgaaaat agaaatacca tcactgagag ataggacaat gattgaaaga
     9421 atagagttaa tcaaaaattt tttcaaagaa gaatcaaaaa gcatttctat ttcaataaaa
     9481 gtacataaag aaataattaa atcactatta ctttatgatt gtaaaaataa tgtaggtcaa
     9541 cttcaaaatg atataaaatt agctgttgca aatggatact taaagtataa aagagataat
     9601 aaagaaccag tttatataga gaaaaaatat tttgataaat ctataaatga agtgagtgat
     9661 gattatagag agaagtctat taaagcagat gaagttataa cacatagaat agaatatttt
     9721 gtgtttacgt cattaggaga agaagaagtt attaattaca atgatataaa tattattaat
     9781 gcagtaaata ctaatattaa taagtctgta gaaatgaata taacagatgc ttctttattt
     9841 aataatgtta agtttaaaaa catgtgtgaa tcaataagac aaattattaa agaagatgaa
     9901 gaaatagata taagtgaatc aatatttaag tcaatggcaa tttatataaa aagcgtattg
     9961 gataaaagta gctctacaaa caacatagat ttgaatcaaa ttagaagaaa taatagaagt
    10021 gaatttaaaa cagcattaaa aatagttgga ataattgaaa atgaatttga tttattttta
    10081 tctatagaaa ctgtagcata tattacatta ttcatagtac aagctaaaca agatgagcaa
    10141 aataaaatag tgaataacat taatattata gttgctatgc atggagaaac tacagcaagt
    10201 agtatggtca aagcagttgg tgatattatt ggaaattgca atgctatttc ttttgatttg
    10261 aagttagata aaagctatga tgaagttatt gtagatttta aaaatttaat taataatata
    10321 gacaacaagg atgtactact atttacagat atggggtcat taaactcctt tgatgaaatt
    10381 ataaagaaag aaaaaaagtg tggagtaatg gtaattccta tggttacaac attaactgtg
    10441 ttagaagcag ttcagaaagc taatatggga ctacctttaa atgatgtata caactctatt
    10501 actaacacga gaaagtacta ttttggaact aatgaaattc aaaataaaga aaatctgtct
    10561 aaaacaatta ttatagcatc acacgtttca gaaggtgtag ataataaaac aagaaaaata
    10621 ttagaagaaa agatgaatag gtatttagat ggaatagata ttatatctgt cccatataaa
    10681 acagaaaaag acttaagttt aaatatcaca aaacttaaag aaagtagtaa tattgtagca
    10741 ttaatcaatg aacaaagaat taatattaga ggaattgact atattagcaa aaaagatata
    10801 gataaggata aaagtatcaa taaattaaaa aatattataa agataagtat aggttatgat
    10861 gatgtagttg aaggtctaaa gacatcctta aaaagctcta attataatag gatatttaaa
    10921 gatataaaat atgtgtctga cgaattgttt ttagtattta acatagaaaa gaaatatgat
    10981 aaagtaattg gacttatgat gcatttagct tttatggtag atggattgat tggaaatact
    11041 agagaaattg aaaagcttga taaagaaaaa atgcttaatt atcacaagag cttaagtaaa
    11101 ataaaagata tagtatctca attagataaa aaatataata tagaaattaa tgaaaaagag
    11161 tgttatcaaa tattgttaat attagaatat gcagaaataa tcgaaaaaga ttaccaatga
    11221 gtataaaaac ttatttggta atttttttag tctaaaataa tattaatatt agacacatat
    11281 gaatatactt agacaaagta tctaagtttt tgtgctaaaa atgactgaaa taacaccgtt
    11341 ttaaagtgtc taaaaaaatt ggcataatac ttgcttatat tactaaatta gtaatataaa
    11401 taattaaaat ttaaataaaa aggagtaaat aatatgcaag ataaaatatt aagagataaa
    11461 aaggcaaatg acccatgggc aagaatgact acaagaaacg gatttagtgc agacgaaata
    11521 atatcatcat ttcaaaaagc tataagaaga aatatggtgg aagaagcttg tgaatttgct
    11581 tatgaactat atatatcatc accagaatta gaagataagc tatggagaag aatacttaca
    11641 atatcagttg aagatatagg aatgggggac ccatcagcta taataatcat aaataattta
    11701 aatcaagtaa gaaaggaata ttcatatgca gatggagata gacctctatt tttcatacat
    11761 gcaataagat atctttgtgc atgtgagaaa gatagatcaa gtgacttatt aaaaaatata
    11821 attataaaag gatttgcaat gggtaaagtt ccagaaatta tggacgtggc acttgataaa
    11881 catacatata gaggattaga aatgggaagg gattcattcc acttcttaaa tgaagctagc
    11941 atagtaatac cagaaaaaga agtagataat gattataaag aaagatactt aaaaatatta
    12001 gaacaatata attcagaaga agttatagat acagctttca gatttaatca ttggcagtat
    12061 taagaattag agtaaatatt aataagaatt aaaataaaaa ttaataagaa ttagagtaat
    12121 aattaattaa taaaaaatta gaaaataaaa gaggttagat attgcaggag ggtaattatt
    12181 atgaatattt tattggtatg tgcaaatgga gcttcaacag gggtgcttgt ggaaaagatg
    12241 aaaaattttt gtagtgagca tgaaaagtta aaaactaaaa ctataaatat agaggcaaca
    12301 tcatttgaaa atctaaaatc atacattcaa gctaatgata cagatgtagt acttgtagca
    12361 ccacaaatta ggttcaagga agatgatgta gttgaagctt gtaaaaatta caaaatagca
    12421 gttggtttaa tagatactaa gcattatggt cgtatggatg ctccttctgt tatgaagtcg
    12481 gcaatcgaat tatataaaaa tagataattg gggggaataa atatggaagc acttcaaaat
    12541 aatttaagaa aatttcttct tccaatagca caaaagattg aaaaacaaag gcatcttcaa
    12601 gctataaaag aaggtatgat aagtattacc ccaataataa ttgttggttc tttaagtatg
    12661 ttatttatgg cattaaacaa tatgttacct gaaggaagtg caaaaacatt attatctgaa
    12721 aatatggata cattacttat ccctaataaa tttactatga gtttattatc catatattca
    12781 gcatttttta tagctcaggc actagctaaa aaatataatc ttaatcatgt agaaataggt
    12841 atgacagcag ttatagcaca acttgttgta tgtggacaag taatagatgg tgcattggat
    12901 acaagttatt tagatgcaca aggtttattt gtaagtatat tagtagcact tttagtagtt
    12961 gatattacta aatttatgaa tgataaaaat ctcgtaatta gatttccaaa agaagtacct
    13021 agtgtagtaa ataaaagctt tagaaactta acaccaatga tagtatgtat aatgttattt
    13081 acagcaatag cagcaataac taaaaatgtc ttaggtcaac cgttaccagc tattataatg
    13141 aatttcttag caccagctat aagcagtgta gataatgttt ttgcagtaac tataatacta
    13201 tttataactc aattattatg gttctttgga cttcatggag cagcaataac ttcaagtata
    13261 tggatgccaa tagcagctac atatatggca gaaaatgcaa cactaatagc cgcaggagga
    13321 gacccaaaat atgtatttac aataggtttt tactatggtt tcttgcaagt aactggttcg
    13381 ggaataactt tagggttagt gtaccttatg tcaagaagca agtgtaagag tttaaatagt
    13441 atgggaaaag ttgtgatttt accatcatta tttggaataa atgagccagt aatatttgga
    13501 acaccaatag ttatgaaccc atatatgttt gtaccatttg tatttggacc tgtattggtt
    13561 ggagcactta actttatggc attaaaggta ggattagtag gattacctat tgcagaacca
    13621 ccaggctttt tacctccagg ggttgcagca tttttaatga cattagattg gaaggcaatt
    13681 gtattagtat ttgcaagtat aatacttatg accttgatat attatccatt ctttaagata
    13741 atggagaagg aagaattaaa taaaaatgca gaattagcta cagcattaga tgatgatagc
    13801 tttgattttt aacaagtcat tagaaggaga aggatatggt acctgaaata gaagaaaaag
    13861 tatttagtat aatatcagaa gcaggagatg ctaaaagtga tgtaatgatg tctcttaaag
    13921 aaataaaaaa aggtgactac aacaaagcaa aacacctttt aaattcagct tcagaaaaaa
    13981 tacaaacggc atcaaaacat catttagaat tactatcaaa tgctatgaat agtgaagata
    14041 gtggaactga ttttttagtt gtgcattctg aagaccatta ttcaaatgca ttatttgctc
    14101 attctttagt atctgaactt gtagatatat ttgagatgat ggacccaaga attaataaga
    14161 attaaatttc ttgtaaatag tgcttgaagt ttggctatag tttattaaat tagtagattt
    14221 aataaatgaa ctccctttta ctttaaagta ctagtattta taaatttatt tataaataca
    14281 gtaaattttt aaatagaaaa ggagatatta ttatggaaaa attttcagta gtaatagctg
    14341 gaggaggaag tacatatacc ccagaaatca tattaatgtt gctagataac ttagataggt
    14401 taccattaag agcaattaaa ttatatgata atgatgaaga aagacaaaat aaggtagcta
    14461 aagcttgtga aatactaata aaggaaaaga atcctaatat agagtattta gcaacaacat
    14521 gccctaaaga agcttataca gatgtagatt tttgtttagc tcatataaga gttggaaaac
    14581 ttgaaatgag agaattagat gaaaaaatac ctctaaaaca tggggtagtt ggtcaagaaa
    14641 cttgtggacc aggtggaata gcttatggaa tgaggtctat agaaggtgtt cttgaaaaca
    14701 tagactatat ggaaaagtat tctccaaatt gctggatgtt aaattattct aatccagcat
    14761 caataatagc agaagctgtt agaaggttaa gacctaattc tagggtgata aatatttgtg
    14821 atatgccaat aggtatggaa cacaatatag caaggattgc agggcttgaa tctcgtaaac
    14881 atatggatat aaggtacttt ggattaaatc actttggttg gtatacttcg attaaagata
    14941 aaaaaggaaa tgaactttta ccaaaattag tagagcatat aaaagaatat ggttttataa
    15001 atggtgaaga aggaatgaaa aatgacaaga aagatagttg gtttgaaact aatttattta
    15061 ccaaagaaat agtaaagaca gatttaacga caataccaag cagttactta aaatactatt
    15121 tatttccaga ttatgttgta aatcattctg atgtaaatta tactcgtgcc aatgaagtta
    15181 ttgatggaag agaaaaagaa gtttttggtt catgtgcatt gatagaaaag caaggtcatt
    15241 ctaaaggtag tgggcttaag ataggaatac atgcagagtt tatagtggat ttagctacag
    15301 ctctagcttt taatactaaa gagcgtatgt tattgatagt tcaaaataat ggagctatag
    15361 aaaatataca agatgatgct atggttgaga ttccatgtat agtggggaaa gatgggtatg
    15421 aaccattatc aataggggaa atcccaacat tccaaaaagg tctaatagag caacaattag
    15481 cagttgaaaa attagtggtt gatgcttgga ttgagaagtc gtataataag ttgtggcaag
    15541 cacttatgct atctaagaca gttccgagtg caacagttgc aaaagaatta ctagatgatt
    15601 taatagaagc aaacaaaaat tattggccag aacttaatta aaaataatat attttttaaa
    15661 gttcctacag tttagtacat agcatagtta atgtatttgt aggaacttta tttatgattt
    15721 tataagtttg tctatgacaa tcttatttct tcaagacttg taaataattt gttatttata
    15781 gtattaataa acttgctaaa caagtagtat ccgtatttac agatgatata caagtagcaa
    15841 atatagtgta ttttacagtt aggatggtag gatttagtta cttgttagtt ggattaaatt
    15901 tatgtacaat agtttacttc aatcactaga aatagtttga gaagttgtat aataaaaaat
    15961 ttacactttt aacaggattt atgtaggtgt ttatagaaat aaataaatat gtacataata
    16021 cataattatt tttaatccta aaatacaaga atggagagat ggctatgaag tggagagatt
    16081 atgctgatga tgtaaattta tttgaggatt ttgataaaac tatcaagcct ctaactgacg
    16141 aacaaagaag aaaaaatata aacactttaa ttgcatattt ttcaaaagaa gttccttcta
    16201 aaatatacaa tttatcaaat gatgaaatta agcctagaga tatacttaga ggacttttga
    16261 atgtatatcc accaaaagaa attgctccag agatacttaa tatgttacat aatttattgt
    16321 tgattgaatg tgaagaaaga gagttagtag atgtaaatga tatagaagaa gttgaggaag
    16381 ggattgccat atggagaggt aatataacga atttaagagc agatgcaata gtgaatgctg
    16441 caaataacaa gttattaggg tgtttacaac cattacatct atgtgtagat aatgagatac
    16501 attcatgtgc aggacctaga cttagagaag attgtaacaa aataataaaa aaacaaggac
    16561 atttagaata tactggggat gctaagataa ctagaggtta ttgtttacct gcaaaatttg
    16621 tagtacatac tgttggacct attgtgtctg gtggtcagcc aagtaaagag caagaaaagc
    16681 aattacttca ttgttataaa tcatgtttaa atacaataaa agaaattgat gaaataaaaa
    16741 atattgtttt ttgtggaatt tctacaggtg tatttggtta tccaaaaaaa gaggcagcta
    16801 atcttgctgt aagtagggtt agattatggc taaaagaaaa ccctgaaaaa aatttaaaag
    16861 ttgtatttaa tgtttttact gaagaggaag aagagaaata tagaagaata tttaaataat
    16921 attatataac aaatcctaaa aatacttatt atataatcct ataatacaaa ttcattgtat
    16981 aagataataa tttgcaagta gtaaaaatta ataaaatata atttgtattt ttaataaata
    17041 agtaggtata atagaaatta taagaataat ata
//
LOCUS       DAMJVM010000072        16720 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.075, whole genome shotgun sequence.
ACCESSION   DAMJVM010000072 DAMJVM010000000
VERSION     DAMJVM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16720
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.075"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            85..1038
                     /locus_tag="PC523_002570"
     CDS             85..1038
                     /locus_tag="PC523_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF2476080.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RVPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFMFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            1028..1984
                     /locus_tag="PC523_002571"
     CDS             1028..1984
                     /locus_tag="PC523_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF2476081.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFVIYGIDINHLEYALSQRIPKI
                     FAMILGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAIMIGASLLLYKMLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKVVKKMIILIAILVSISTALIGPMTFLGLLLANIAREIFKTYKHTYLILGSMLIGM
                     ITLITGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            1981..2736
                     /locus_tag="PC523_002572"
     CDS             1981..2736
                     /locus_tag="PC523_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2476082.1"
                     /translation="MIEIKNIFKKYKNKNVVDDVSFNIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRGLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMDFKIKNINGNRICIYF"
     gene            2828..3793
                     /locus_tag="PC523_002573"
     CDS             2828..3793
                     /locus_tag="PC523_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2476083.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKNNESKTSENSNNTIKIIH
                     NLGETDVKLNPKKVVVFDYSALDTMDTLGVAENLVGLPKASLPASLEKYKDEKYADLG
                     GLKEPDLEGIKSTNPDLIIINGRQEDFYEQLSKIAPTISTSKDDKEYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFKNKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDIKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
     gene            4247..6106
                     /locus_tag="PC523_002574"
     CDS             4247..6106
                     /locus_tag="PC523_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2476084.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHKPNDELIEYIDEILQELITGHGNYGLVLTDCNKYNEDLLLLEKKW
                     EDLNSEIKKVRMKEPNNQLLSISEEFFSLANDTVFEIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMIKLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICMHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YIDKEDLVVRQRIVDSELINTTNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHNQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQNHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDALKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIEYEEQVEFLRDIGCDLVQGFVFYKPMPILEFEEILDKEFVYNS"
     gene            complement(6306..6905)
                     /locus_tag="PC523_002575"
     CDS             complement(6306..6905)
                     /locus_tag="PC523_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2476085.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNAQGENLRIN
                     GNKIKEIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(7008..7805)
                     /locus_tag="PC523_002576"
     CDS             complement(7008..7805)
                     /locus_tag="PC523_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2476086.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(7918..8023)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(8115..9101)
                     /locus_tag="PC523_002577"
     CDS             complement(8115..9101)
                     /locus_tag="PC523_002577"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDF2476087.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMNYLSKTNTDNTNYCLSELD
                     IEQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVSFVENKLIGIRHEENALRNTLNSIEINDYFLGADTDILVSGILGVK"
     gene            9585..11315
                     /locus_tag="PC523_002578"
     CDS             9585..11315
                     /locus_tag="PC523_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDF2476088.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIIISALLLKEFGPMRKAEIKSRSRKIDI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGEDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVIKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKR
                     VDEAEIELVE"
     gene            11425..11505
                     /locus_tag="PC523_002579"
     tRNA            11425..11505
                     /locus_tag="PC523_002579"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11459..11461,aa:Leu,seq:tag)
     regulatory      12284..12388
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            12598..15072
                     /gene="gcvPA"
                     /locus_tag="PC523_002580"
     CDS             12598..15072
                     /gene="gcvPA"
                     /locus_tag="PC523_002580"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HDF2476089.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHDMVRK
                     SAGIFDVSHMGEVQIKGVESEKFIQNLVTNDISTLNINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSERYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSVKVCGCPCLVSRTGYTGEDGFEIYCENKYAEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPVEGGLSIFVKTNKESFIGKSILSK
                     EKENGAKRKLVGFEMQGKGMPRNGYDIRIGDKIVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIAKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATSAIEACIMAMNQTRKNKIVVSKTTHPETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDTEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAIQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            15072..16529
                     /gene="gcvPB"
                     /locus_tag="PC523_002581"
     CDS             15072..16529
                     /gene="gcvPB"
                     /locus_tag="PC523_002581"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HDF2476090.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIEDMIDQNMIRKSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLSEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGNMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVSFLPIPVIEKREDKYILNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIDAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
BASE COUNT         6431 a         1903 c         2730 g         5656 t
ORIGIN      
        1 atgataatta ttattgataa actattgaag tgaaaatgat aataattttc attttaatta
       61 ataaaagatt tttaaggagg gagaatgaaa aaaaggtatt tagtcatagg aataataatt
      121 ttatcttatt tttctttatt tataggagct gaagatataa atataatgca tatatttgca
      181 aaaaatcaac ataagcttat gatatttatc atgagtagag taccaagatt aataagtata
      241 ttgattgctg gtgtaggaat gagcatagca ggtcttataa tgcaacagat aagtaaaaat
      301 aaatttgtat cacctacaac aggagctaca atagacgcag ctcaatttgg gatagtgata
      361 tgtatgcttt tagtaccaac tgcctctatt tttacaaaga caataatagc atttgtattt
      421 tctcttgtag ggacttttat gtttatgaaa ataataggta agttacaatt taaaaatata
      481 atctttgtac cattagtagg tataatgttt ggaaatataa taggttctat gacagatttt
      541 atagcatata aatacgattt gagtcaaaat gttagctctt ggatgcaggg tgacttttct
      601 atgatactta aaggaaatta tgagatatta tatataacaa taccccttat aatactagca
      661 tatatttatg caaacaaatt tactgttgtt ggtatgggta tggattttgc aacaaactta
      721 ggtcttagtt acaaaagaat tgtcaatata ggtctgataa tagtagcact tgttacagta
      781 tgtgtagttg ttacagctgg taatatacct tttataggtc taatagttcc aaatatcgta
      841 tccttatata tgggcgataa tataagagca agtatatggt atacaggttt acttggagct
      901 atatttgtac ttatatgtga tatttttgga agaattatta tttatccata tgaaatatct
      961 ataggactta cagttggagt tataggaagt atactattcc tttatttaat attaaggagg
     1021 aatgtaaatg aagcttagtc tcaatcaaaa aatttacttt gtaagtatat tactattcat
     1081 atcaatagca ctttttgtaa tttatggaat agatataaat catttagaat atgcattatc
     1141 acaaagaata ccaaaaatat ttgctatgat attgggtgga ggatgtatag catttactac
     1201 tgtagtgttt caaacaatta caaacaatca aatattaaca ccaagtgtac taggtttaga
     1261 ttcattatat gtaatgattc aaactataat agtatttata tttggttcaa gtagtagtct
     1321 gattataaat gaaaattata attttattat taatgtagct attatgattg gagcttcatt
     1381 attattatat aagatgctat ttgaaaaaaa taaaaataat attttctttt taatcctagt
     1441 aggaatgata tttggaactt tatttaaaag tgcaacaaca tttattcaag taatgataga
     1501 cccaaatgaa tttttagctt tgcaaactag cataatggcc agtttaaaca atataaacac
     1561 taatgtattg ttaatagcat ttataataat tattgccata atacctttta tatatgatga
     1621 aataaagtat ttagatgtat tgtcattagg taaagagcaa gcaattaatt taggtgtaga
     1681 ttttgataaa gtagttaaaa aaatgataat attaatagca attttagtct caatatcaac
     1741 agcacttatt ggacctatga catttttagg acttttactt gccaatatag ccagagaaat
     1801 ttttaaaaca tataaacata catacttgat tttgggctct atgttgatag gaatgattac
     1861 attgataaca ggtcaatttt ttatacaaca tgtatttaaa tttgatacaa ctttaagtgt
     1921 agtaataaat tttataggag gaatttactt tattcaatta ctattaaagg gggcaaatag
     1981 atgatagaga taaaaaatat atttaaaaaa tataagaata aaaatgttgt agatgatgtc
     2041 tcttttaata tagaaaaagg aaagataaca tcttttatag gaccaaatgg agctgggaaa
     2101 agcacagtac tatcaatagt tacaaggctt ataggaggtg atggtggaga agtaataata
     2161 gaaggaaaaa gcttgactaa ttatagcaat aaagaattgg caaaaaaaat agctattctt
     2221 aagcaatcta acaatataac tttaaagctt acaataagag aattagttgg atttggaaga
     2281 tttccatata gtgaaggtaa tctaactaaa gaggatgaaa attatattga tgaagcaata
     2341 gaatatatga aattgactga tatacaacac aagtatttag atgaattaag tggaggtcaa
     2401 agacaaagag cttatatagc aatggtaata gctcaagata cagagtacat attgcttgat
     2461 gaaccactca acaatttgga tatgaaccat tcagtacaga tgatgaaagt acttagaggt
     2521 ctttgtgatg agttagataa gactatagta ttagtaatgc atgatataaa ttttgcatca
     2581 tgttattcag ataatatagt tgcattaaaa aatggaaaag ttgaaaaagt aggtcgtaca
     2641 gatgagatag taaatgaaaa agttttagaa gatatttatg agatggattt taaaattaag
     2701 aatataaatg gaaatcgaat ttgtatttat ttttaaaata ttatatataa aaataaactt
     2761 gctatattat tttttataaa atttttaaag taaaataaag attaatagaa aagaggaaaa
     2821 tattgatatg aataaaaaag cagcgatagt agcagcagta gctattatag gtttagtgac
     2881 agtgtttgca ttaggtggaa gtaaaaataa tgagtcgaaa acatcagaaa attcaaataa
     2941 tactataaag ataattcata acttgggaga aacagatgta aaattaaatc ctaaaaaggt
     3001 agtagtattt gattattcgg ctctagatac aatggataca ttaggtgtag ctgagaattt
     3061 agtaggactt ccaaaggcga gtttaccagc atccttagaa aaatataagg atgagaagta
     3121 tgcagattta ggaggtttaa aagaaccaga tttagaaggt ataaagtcaa caaatccaga
     3181 tttaattata ataaatggaa gacaagaaga tttttatgag caattgtcaa aaatagcacc
     3241 aacaataagc acaagtaaag atgataagga gtacttagaa tctgttaaaa ataatataga
     3301 taaaatagca aaaatatttg gagtagaaga aaaagcaaat caagaattta gtaaaattga
     3361 aaagaaaata gaaactttga ataaaaaagt aacagataaa aatttaaatg ctttaactat
     3421 aatggtgaat gaaggtaatt taagtgtatt tggtgaagaa tcaagattta gtatattata
     3481 caatagtttt ggatttaaaa ataaagataa aaatataaaa gaatcaagtc atggacaaaa
     3541 tattactttt gagtatatag ctaaacaaaa tccagaagtt atgttcgtta tagatagagg
     3601 aatagctact ggtagtgata taaaagagag ctcaactgca aaatctgttt tgaacaatga
     3661 tataataaaa tctatggatg catataaaaa tgataatata atatatttag attcaccaac
     3721 atggtatgta aatgatggtg gtctaacatc tttaaataaa atgatagatg atgcttcaaa
     3781 agcggtgaat taataatctg atataatcaa aatgtgtttt ttatgaaatg attatcaaat
     3841 aaactaggag ttcttaagaa ctcctagttt attttttgca taataaagat atagtttaaa
     3901 aaataatatt atttacaata agataaattt attcaaaatc taataaaata tgaagttata
     3961 ctagacaaaa agaataattc ttaaatataa ttaaattata tggaatatat aataatataa
     4021 ttttaataag ttgtttataa tatataaatt atattaatag taataattta tatattatga
     4081 gaaatttttg tatatataga gcactctaaa gatgaactat gtaaaacaag gtttatatat
     4141 tctaattgtg tagaaaattt tattggaaat gaatgcatat tattagaaaa atagtttaga
     4201 gatatccaat aaacataaaa aataatatag gggataaggg tgttttatga agagattttt
     4261 gaggagaatt atcttagttt tatttatttt attgattttt ataagtttca tttctattaa
     4321 attaattcat aatgttggag attatgggaa actgattaat tatgttggta ttgttcgtgg
     4381 agctagccaa cgcctaacta aactagagat gaatcataaa ccaaatgatg aattaataga
     4441 atatattgat gaaattttac aggaactgat tacaggtcat ggaaattatg gcttagtact
     4501 cactgattgt aataagtata atgaggactt gcttttattg gaaaaaaagt gggaagattt
     4561 aaatagtgaa attaaaaaag taagaatgaa agaaccaaat aaccagttat taagtataag
     4621 tgaagagttt ttttctcttg caaatgatac agtgtttgaa atagaaaact tttctaaaga
     4681 aaaaagtaat tacttaatga ctttaataat aattatatct attataggca tattagcatg
     4741 tatcatattg attttacagt atagtaaaaa aatgattaaa ttagaaaaat taaatgtaga
     4801 tttaaaaaat attgcatata aagatgaact aacaggagta aatactatag aaaaatttaa
     4861 attggatgca aatcaaaata tttgtatgca taaggataag aaatttgctg tgttctatat
     4921 tgattttgaa aactttaaat acattaatga tatatttgga tatgattatg gtgacatgat
     4981 actgaaaaga tatgctaatc ttatgatgaa tgatatagga aaatatgaaa tatttgcaag
     5041 agaaatagca gatagatttg ttgcattaag atgctatata gataaggaag atttagtagt
     5101 aagacaacga attgtagata gtgaactgat taatacaaca aatgagatta aaaataaaca
     5161 tagcattact gttgttagtg gaatttgctg tattgaagat gtaaatgaaa aattaagtat
     5221 agatggctta ataaatagag cgaactttgc acaaaaaact gttaagaata aaccaggtac
     5281 taattatgct ttttataatg atagtattcg taaaaaaatg attgaagaaa atactatcaa
     5341 gagtagaata catgaagcta ttgaaaaaag agaatttata gtttatttac agcctaaagt
     5401 caatttacat aatcaaaaaa taaattgtgc agaagcctta gttcgttggt tgactccaga
     5461 caaaggtatt atatctccag caatatttat tccagtatta gaaaagaatt tttttattgc
     5521 tttagtagat aaatatgttt ttgaggaagt ttgcaaatgg attagaaaga gattagatga
     5581 aaataaacct tttgttcaga tatcagttaa tgtttccaga atacaatttt ataatactaa
     5641 gttcgtagaa acttattcca atatacaaaa taaatacagg atacctaaaa atactataga
     5701 aattgaattt acagagagtg ttgcttttga gaatcaaaat catcttttgg aaattataca
     5761 tgacttgcac gaaaatggat ttacttgttc tttagatgat tttggaaaag gttattcgtc
     5821 tttaagtgtt ttaaaagatt tgccatttga tgctttaaag cttgactcta tgtttttcaa
     5881 ggctagttta gataaagata aagaaaaaat agtaataaaa aatattgtac atatgttaaa
     5941 ggagcttaat atcacaactg ttgctgaggg tattgaatat gaggaacaag ttgaatttct
     6001 tagggatatt gggtgtgatt tagttcaggg atttgtattt tataaaccaa tgccgatatt
     6061 agagtttgaa gaaatattgg ataaagagtt tgtttataat agctaaaatg tactttgtaa
     6121 aatacaaact aattaagtaa aattattcta cactgaacaa attttcattt tatttaccat
     6181 ataagaatgc gtaatgatat aagtatttga aaagccacca ttatatgaaa attaaaaaga
     6241 aaataaataa tttttcgata aaaaagagac tatgagaaaa gtcacccata gtctcctaaa
     6301 tatagttatt ttcttcctgc aacccatttt aagccccaat gaaattcagt atccatttct
     6361 ctatgacctt tgaagtattt aactactttt tcaacactaa atgtctcatc atttacattt
     6421 tctatcaatg ctattgaaca catattatag ccatttttat gttcatcaag tttgacaact
     6481 aagtctgaat cttgtttttg tttgattgtt acaattccat cagcttcaga ccagttagcc
     6541 acaccttcat atataaaagc ataaataagt attcttttta tctcttttat tttattacca
     6601 tttattctta aattttctcc ttgtgcatta cttcctgttc ggtcatctcc atctaattga
     6661 gcaaaaggtg gattgtctaa actaccaaaa gcatttccta atgcttgaac agcacctttt
     6721 acaccatttt tcatctcaaa taggcaaccc aaatctaaat ctatattact gcttcttaaa
     6781 gagccaaaaa aacccttttt ttgtactttt tgattccaat ttaaattaac taagatttca
     6841 cctaaatcgc tattttcctt ttttgtaagg tttatttttt gtcccttttt taattctata
     6901 gccatagtaa tcttcctcca caaacataga tttatttaaa tggtagcact taataagtca
     6961 atattcaaca agattagaat aataatatta aaattatact aaatatacta aaaagtagga
     7021 attacaatac ctttgtattt ttcttcaatt aagcttcttg ctttatctga tgtaagaaca
     7081 tcttgtagaa ctttaatctt ttcactatct ttatcttcag ttctaaatgc taaaagattt
     7141 gcatactcat catttatagg agctgtataa attccgtttt tcttggcatc aagaccagca
     7201 ggtacagcat aagtagtatc tataaatgca gcagttacat ctggaagaga agatggaatt
     7261 tgagcaatat ctaactcttt gaattgtatt ccctttggat tttctattat gtccttaggg
     7321 ctaactaatc catcaccttt aggcaatgtt aaaagtccaa tactttcaag gtatctaagg
     7381 ttcttagaac aagaagaagg attattgctt attgcaattg tatcaccttt tttaaattca
     7441 tcaacagatt ttatcttata agagtaaagt attgctgggc atacatatag ttttttacca
     7501 gccattattt tgtaaccttt ggccttaaca gcttctttta gataaggttc atgttgaaat
     7561 agatttgcat caatctcacc attatttaaa gcttcgtttg gaagtatgta atcatcaaag
     7621 tttttaacct ctaaagtata tcctttttcc ttaataagtg gttttaattc ttctaataac
     7681 tctccacctg gaactaaagt agcaccaaca acaatttttt tatcatcttt agctttagaa
     7741 catccaacca cagctaaagt taatactaga cataataata aacataataa ttttttgaat
     7801 ttcatataaa ttccctcctg aatataattg attagttaaa aataaaatac tattctttat
     7861 cagactgcaa aataaaaata agcctgagtt taaactcagg ctttaaaagc taatttagac
     7921 tcatctttca gtataatact gcaggattta gcaccaatgt atataaaaat atactggttg
     7981 ctgggtttca tagggccagt cccttcacca ctctggataa gaagtatcaa tttttaatta
     8041 tgttataaat attatcatga ttttttacgt ctgtaaatat aattttataa atattttaaa
     8101 atctttagtg tattttattt aacacctaaa attccagaaa ctagaatatc agtatcagca
     8161 cctaagaaat agtcgtttat ttcaatagaa tttagggtgt ttctcaaagc attttcttca
     8221 tgtcttatgc caataagttt attttctaca aatgaaacat cacattttcc aaaaaaatct
     8281 ccaaaaaatt ttatgctagt tattatgcct ttatcaacat ttaaattaaa ctcaacattt
     8341 ccaccagaat attttaattc atttgaaaga gtatattttg gtgaattacc aaaattccaa
     8401 tcccaagttt catattttga tttagtaagc ttttctattt gttctatatc aagttcactc
     8461 aaacaataat tagtattatc agtgtttgtt ttagaaagat agttcattat agcatcttta
     8521 aattctaata tgtccatttt agagtgtaaa tgttcactta tattggttac acgagatttt
     8581 acagatttta ttccttttcc ttcaaatttt ataggtttaa cttttaatgc ttttgagagg
     8641 tcatttattt cagatgaaaa taaaagtgta ccatgatgca ttactttgtt tttgtagttg
     8701 tactgagcat ttccagaaaa tttcttgccg tctataagaa ggtcatttct tccagaaaat
     8761 tcagcattga catctaatgt tttaagtaag tctattattg gttgagtgaa tcttctgaaa
     8821 tcactaaaat tattattgtt acaagctata aatgtaaaat ttatatttcc taaatcatga
     8881 aatacagccc caccaccaga ctgtctccta acgacaggta tagaatattc ttttatatat
     8941 tctaaattta tttcagaaag tgtgttttgg ttttttccaa caattataga aggttcattc
     9001 ctccataaaa tgaataggtc ttcatcaaaa ttttttagaa gatattcttc cattgctaaa
     9061 ttaaaataag ggtttgtttt ttcattataa attaataaca ttaaagtagt tcctccatat
     9121 aattgataaa atttttatta tgagtacaat taattttata attcgacaat aatgtaaaaa
     9181 ctcctttaaa ttagaaattt taaaaactac cttgcaaagt ttaaataaat aatatatact
     9241 caaaatatgt ttaataaaag tgaatagaag ggtagaggcg caataatcta tcagtactaa
     9301 taaggagtta agcacataga gttattagga aaggagatat tgccgaagtt ttataatcga
     9361 tgctttaaga ttatattgct gggggtacat agaaaatatg tacaactgtc acaggtttat
     9421 ttgtggtgag ctatcattta aggaattata ttatatttat ttttactatt gttgatatat
     9481 tgtaatgttt aagccttgag tgtttaccac tcaaggcttt tttagtttta ttgtaacata
     9541 ataccctata cagttcacaa aattaaaagg agagggagat ttatatgagg aacaaaaaaa
     9601 ttaatattat ttttctgacg acaatcatgt tcataatgtc aacagtaatg gtttttgcag
     9661 aagaggatat agacactatt gcactagcta atgcagaaaa atttggaatt ttaactttaa
     9721 taccaccatt agttgctata atacttgcat ttataactaa aaacgtaata atttctttac
     9781 ttataggtat tctttctggt agttttataa ttaaagctag tggtattaat gtttttgcga
     9841 catttataca agcattttta gatttagtag acagagcttt agtatcttta gcagaccctt
     9901 ggaatgcagg aataatacta caggttttgg caattggagg agtaataaat cttgttgcta
     9961 aaatgggagg agctaaagct atagctgaag cacttgctaa aagagcaaag tcagcaaaag
    10021 ggacacagct tataacatgg tttttggggc ttttagtatt ctttgatgat tatgccaatt
    10081 cattaatagt aggtcctatg atgagacctg ttgcagataa gatgaaaata tcaagagaaa
    10141 aactagcctt tataatagat gctacagcag caccagtagc tggtttagct ataatatcta
    10201 catggatagg tcttgaggtt ggtttaatac atgatgcttt tgaaagtata agtatagatg
    10261 tagatgcatt tggtatattt ttaaatacaa taccatttag attttacaat atattaatat
    10321 tagcattcat tataatatca gccttattat taaaagaatt tggaccaatg agaaaagctg
    10381 aaataaaatc tagaagtaga aaaattgata tagaccttga tgaaggcgta gaagagttgg
    10441 atgatttggc tccaaaaaat ggagtgaaat tgagtgtatg gaatgctata atcccaatag
    10501 gaactttgat aatagttgca ttagcatcat tttattatag tggatacaca tctataatgg
    10561 gaggagagga taaggctctg atacaactat ttacaaattc tccatattct tttgaagcaa
    10621 taaaagaagc atttagtgca tctgatgcat ctagagcatt attccagtct gcacttgttg
    10681 caagtttagt tgctattata atggctgtaa taaaaaagat ttttacaata tcagaagcaa
    10741 ttgatgtttg gatagatgga atgaaaagtt tagtaatcac tggtgtaata ttaatacttg
    10801 catggtcatt aagttcagta ataaaagagt taggtacagc taaattttta atacatttat
    10861 tgtcaggttc actaccacca ttcttattac caagtttaat atttggttta ggagcaataa
    10921 tatcttttgc tactggaaca gcttatggaa caatgggtat acttatgcca cttgcaatac
    10981 cattagcata ctcattaaat cctgatatgt cttatgtaat agtaagtact agtgcagttt
    11041 taacaggtgc aatatttgga gaccattgtt ctccaatttc agatacaaca atactttctt
    11101 caatgggagc tgggtgtaat catatagacc atgtcaacac tcaaatgcca tatgctatat
    11161 ttacagcagt aattactatt gtatttgggt atataccagc tggacttgga ttgccaatat
    11221 atatagtatt accagttgca atagctgcta tatttgtagg tattcaaata attggtaaga
    11281 gagtagacga agcggaaata gaacttgtag aataaagata taaataagtt tcacctttta
    11341 ttatcattat tttgttagtt tttagataat tttaaaaagt tattgaaaat aatagcgaaa
    11401 tatagtaaaa taaaagagtg ataagcggat gtggcggaat tggcagacgc actagactta
    11461 ggatctagcg ccattggcgt gggggttcga ctcccttcat ccgcacttta cagtacttaa
    11521 ttgtgctgaa aaaaactggg gttgctggct ttcggagtgg gctatcccgt ttttattttt
    11581 tggtatatat gaaagattgg cactttaata tgaagtgctt tttttatagg caaaaaacag
    11641 tacatttatt tctgttttat tgtcattaat ttattagaac atgctttagt taagaatatg
    11701 ttaataagca atgttcacat tcttgataaa atatggaata tatgtatctt tactaaggca
    11761 gaaatgaaat aactaaacta ctaaatgata ttataagatg ataaataagt ttcagtttat
    11821 tggatggttg aagtaatcag aaattggaat tttatagtct taatataatg tatgaaattt
    11881 tatagcaatg tatttcataa gtataaagtg actaaaattt taaaaaatat acaaattcta
    11941 tagaagctaa ataatttcaa ctctatttta aaaatcttat ttttcacaag tttaaaattg
    12001 taaacgattt catcaagtct atgaatatac ttttaacatg ttatagtaaa tctcaatata
    12061 aatttacatt tgcttccttg tatgttattt taataataaa ttaagtgaaa atgttatttt
    12121 acattaatat ataatatttt tgcataagaa tgaataatat aaaagcgaaa taattgataa
    12181 taaaaactta acaaaaaaat taatacataa aaaaaaataa atataatgtg ttgacattta
    12241 attgaaatat tcatacaata aaattgaaaa taaatatttc caaatgagat tagcgagaga
    12301 gtcctttata cgaaaaggcg ccgaagaaga aatttaagaa atgccaattt ttagagaagc
    12361 tttcaggcaa aaatatcgct aattgatggg actctggaga gacttaagtt aagatactat
    12421 aagtcaccga agaagcaaaa taatattaaa ttattaaatc tttcaggtat acaaacaggg
    12481 atatataggc taggattatt ttccatgctg atatatcttt tttttatgaa aaagtacagg
    12541 aaataatatc aaagttactt aaatacagta ggtctaattt tatagggagg gttatatatg
    12601 aatgaattga aaagagtatc tttgtataac atacacaaag agcttggagc aaaacttgtt
    12661 gaatttgctg gctgggaaat gcctttagaa tatgaaggta taaataaaga gcatgatatg
    12721 gtcagaaaaa gtgcaggtat atttgatgta tctcatatgg gagaggtaca gataaaggga
    12781 gtagagtcag aaaaatttat acaaaaccta gttacaaatg atataagcac attaaatata
    12841 aatgacatta tatatacacc tatgtgttat gaaaatggtg gagtagttga tgatttatta
    12901 atatataaat ttggtgaaga agattattta ttggtaataa atgcaggcaa tatagataaa
    12961 gatgtagcat ggataataaa acaaagtgaa agatacaatg tagatataaa aaatatatct
    13021 agtgaagtga gtcaattagc aattcaagga cctaaagcag aagaaatcct tcaaaaaatc
    13081 acagatatag acttaaactc aataaaattt tataagtcaa ttccatcagt aaaagtatgt
    13141 ggttgtcctt gtttagtttc aagaacagga tatactgggg aagatgggtt tgagatatac
    13201 tgtgaaaata aatatgcaga aataatttgg aatgaagttc taaaggttgg aggagaagat
    13261 atatgtccag ctggactagg ttgtagggac actttgagat ttgaagcagc tctacctcta
    13321 tatggacatg aaataaatga acatatatct ccggttgaag gtggtttatc tatctttgtg
    13381 aaaacaaata aagaatcatt tattggcaaa tcaattttat caaaagaaaa agaaaatgga
    13441 gccaaaagaa agttagtagg atttgaaatg caaggtaaag gaatgcctag aaatggatat
    13501 gatataagga ttggtgataa aatagttgga tttgtaacaa caggatgtgc atctccaaca
    13561 acaggaaaaa ttttaggtat gggaattata gattctgagt atgcaaaagt aggaaatgaa
    13621 ataggtatag caatcaggaa aaaagttgtt cctgctgtga tagctaagaa acctttttat
    13681 aaaaaacaat ataaaaaaga taatattata ttaaacaaag aaaataaatt ttcatatata
    13741 cctgctacaa gtgaagataa atcaaaaatg ttaaaagtag ttggattaaa ttcagttgat
    13801 gaattatttt cagatatacc agaagaagta aaattaaaaa gagatttaaa tttggaaatt
    13861 ggaaaatctg aattagaggt aagtaaaata gtaaaaagat tgtcagaaga aaatctaagc
    13921 ttagaagacc tgacttgttt tcttggtgct ggtgcatatg accattatat accatcaatt
    13981 ataaaacata taacgtcaag atctgaattt tatacagcat acactccata ccaagctgaa
    14041 ataagtcaag gaactttaca agtagttttt gaatttcaat ctatgattgc agaaattaca
    14101 ggtatggaaa ttgcaaatgc atcaatgtat gatggagcaa cttctgcaat agaagcatgt
    14161 ataatggcaa tgaatcagac tagaaaaaat aaaatagtgg tgtcaaaaac tactcatcct
    14221 gaaactttgt cagtattaag aacttattta caatataaag attgtgagat agtagaaatt
    14281 gatttttgta atgaatatgg gactacagat atagaaaaat taaaagcttc tgtagataaa
    14341 gacacagcat gtgttcttat acagactcct aatttctttg gaattattga agaaatggaa
    14401 gaaatagaaa agataactca tgaaaacaag gctatgttaa ttatgagtgt agacccaata
    14461 tcattaggag ttttaaaaac accaggtgag ataggtgcag atattgttgt tggagaagcc
    14521 caatcacttg gaaatcctct taattttgga ggtccttatg taggattttt agctagcaaa
    14581 tctaaatata cgagaaaaat gccaggaaga atagttgggc aaagtttaga tacagaaggt
    14641 aagattgcat atgtattaac tttacaaact agagaacagc atgtaagaag agaaaaagca
    14701 acttctaata tatgttcaaa tcaagcttta aatgctcttg tggcttcaat atatatggct
    14761 actatgggta aagaaggatt taaagaggtt ggtatgcagt ctatgaaaaa agcacattat
    14821 acttataata agcttgtaca aactggaaaa tataagccaa tatttaaagg caagtttttc
    14881 aaagaatttg ctatacaagg aaatctcaat atagaaacta taaatgataa actcctagaa
    14941 gaaaatatat taggtggtta taatttagaa tacaattatc cagaattaaa aaattcaact
    15001 ttactttgtg tgactgagaa aagaagcaaa gaggaaatag ataagttagt tggaataatg
    15061 gaggggttat aatgaaagaa tataatagtt tattgataga tatatctaaa aaaggtagaa
    15121 aagcatactc acttccaaaa ctagatatag atgatataaa aatagaagat atgattgacc
    15181 aaaatatgat aagaaaaagt gaattgaatt tgccagaagt cggtgaatta gaattagtta
    15241 gacattatac attattatca aataaaaact ttggtgttga tacaggtttt tatcctttgg
    15301 gttcttgtac tatgaaatat aatcctaaaa taaatgaaga tatggcagca cttccaaact
    15361 ttactggaat gcatccatat caatcttcag atacagctca aggttctctt tccttgatgt
    15421 atgatttatc aagaagactt tcagaaatta caggtatgga tgaagttaca ctacaacctt
    15481 cagcaggttc acatggtgag tttacagggc ttatgattat aaaagcatat catgaaaata
    15541 gaggtgacca taagagaacc aaagtgataa ttccagattc tgcacatgga acaaacccag
    15601 caagtgcagc tatggcaaac tttgatgtga ttcaaatagc atctgataaa aatggagctg
    15661 tagacataaa tgcattaaaa gaagttttaa atgatgaagt agcagctctt atgcttacta
    15721 atccaagtac tcttggtttg tttgaaaaaa atataaaaga aatagctact cttgtacatg
    15781 aagcaggagg gcttttgtac tatgatggtg caaacatgaa tgctattatg ggtataacaa
    15841 gacctggaaa catggggttt gatgtggttc atcttaatct tcataagact ttttctactc
    15901 ctcatggagg tgggggacca ggagctggtc ctgttggagt taaaaaagag ttggtatcct
    15961 ttttaccaat accagtaatt gaaaaaagag aagataagta tatattaaat tatgatagag
    16021 aaaagtctat tggaaaaatt aaaaactttt atggtaattt tggagtgctt gtaagagctt
    16081 acacttatat attaactatg ggaagagatg ggcttaaaga agctagtgaa atggcagttt
    16141 taaatgctaa ttatataaag gaaagtataa aagatgatta tattttgcca atagatactt
    16201 tatgtaagca tgagtttgta ttaggtggat taccaagagg agaggcaaat ataaaaacta
    16261 ttgatattgc aaaaagacta ctagattatg gataccatcc tccaacaatg tactttcctc
    16321 ttattataaa tgaggcactt atgatagagc ccacagaaag tgagagtata gagacattag
    16381 atagctttat agacgctatg aaaagtgttg ctaaagaagc aaaggaagaa ccagaactat
    16441 tgaaaactgc tccacataat actttagtaa aaagggtaga tgatgcaagg gcagtaaaaa
    16501 aacctatact gacatggtca caaagataaa taattaaaaa atagagattt aaagcataaa
    16561 taaatttgaa tttgcaaaaa aagctatcta acttaatcta gtttaggtag tttttttgta
    16621 ttggtagata taagattttc tattaaaaat ttaaattata ttattactca tataaaatat
    16681 taatgtaatg cttatgtaca aatattcata agaattattg
//
LOCUS       DAMJVM010000073        16349 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.076, whole genome shotgun sequence.
ACCESSION   DAMJVM010000073 DAMJVM010000000
VERSION     DAMJVM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16349
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.076"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(602..1240)
                     /locus_tag="PC523_002582"
     CDS             complement(602..1240)
                     /locus_tag="PC523_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2476091.1"
                     /translation="MLIHLVKKDVLIVKKYVLFILFLAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFIMVFIYCTLVY
                     WIENLLIPQMGTFDLTYILIVLLISSIIYGVYMPVQYKLGYETTKAFFVVIIMASSFV
                     LPLIIGHSNFEVPFIYQYSSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            complement(1243..2100)
                     /locus_tag="PC523_002583"
     CDS             complement(1243..2100)
                     /locus_tag="PC523_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2476092.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVIKRDSGKIKIFGKDFNGNEKELKNRIGVVFDDSYFYDELSMAEMKSIIAP
                     SYDNWCEEDYQKNMKHFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTEYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     EEHRKVKGDIKFLNDEIKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            complement(2102..2476)
                     /locus_tag="PC523_002584"
     CDS             complement(2102..2476)
                     /locus_tag="PC523_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2476093.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAS
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYEED"
     gene            complement(2927..4117)
                     /locus_tag="PC523_002585"
     CDS             complement(2927..4117)
                     /locus_tag="PC523_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF2476094.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTSAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAESTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVLVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(4133..6088)
                     /locus_tag="PC523_002586"
     CDS             complement(4133..6088)
                     /locus_tag="PC523_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDF2476095.1"
                     /translation="MEKIKIENSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGIDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENTTKAITPKFCEVAGIDVSSDKKRILYVGQIYEDCATPFS
                     GLWEYHIESGETKELVPQGKYQISLAKYIGENKVMLQASTLDRSITQNHDIFILDLST
                     SEISMIASLDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFDEFDTNTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFIAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDICIEKYPGIDAERLGVA
                     GGSYGGYMTNWIIGHTDRFKAAASQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIVRVNAIVDWFERYL"
     gene            complement(6107..8173)
                     /locus_tag="PC523_002587"
     CDS             complement(6107..8173)
                     /locus_tag="PC523_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDF2476096.1"
                     /translation="MKEVVRSLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENAYTSQVYILNNKSGELKQITSINSVGAYAWEDENTILFPALRN
                     EKVKEAVKDGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLSEEEQQKKINAYNNRHYEHFKEIPYAVNGEGYVSRKRKRLYLYDSNINELKAI
                     TAPMFNVVGMKISDGKILYVGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIVKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSEHQHHCIGNTVTSDVKMGV
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVAFL
                     GDRLPEVYTVENGTLCKKTHLNDHILEEYKISEPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLVIHGGPRLTYGPYFMHEMQVFASAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            complement(8464..9114)
                     /locus_tag="PC523_002588"
     CDS             complement(8464..9114)
                     /locus_tag="PC523_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2476097.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEKEDV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            complement(10242..10835)
                     /locus_tag="PC523_002589"
     CDS             complement(10242..10835)
                     /locus_tag="PC523_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2476098.1"
                     /translation="MNYTIEPTQLNKMYKDKAVINNINIHVRKEVIYGFVVQNRAGKS
                     TVMKMLLNLTKSNSGEIIIFGKKLEETDFEMLKKISTIIENTYFYENLTDKQNLDLHC
                     EYMGYYNKEHISEILKCVGLSKQSSKKVSKYFLGIKQCLLIARAILTKPELLILDESI
                     NALDPEGIREMRELFNKLNTDYITTIFTSSHILSEVE"
     gene            complement(11531..11836)
                     /locus_tag="PC523_002590"
     CDS             complement(11531..11836)
                     /locus_tag="PC523_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476099.1"
                     /translation="MPSMIMEIVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     IGIIAAGIALIFFRNGIDSIGSIATYGIFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
     gene            complement(12561..12779)
                     /locus_tag="PC523_002591"
     CDS             complement(12561..12779)
                     /locus_tag="PC523_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2476100.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENDI"
     gene            complement(12780..13187)
                     /locus_tag="PC523_002592"
     CDS             complement(12780..13187)
                     /locus_tag="PC523_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476101.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKLLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            complement(13273..13761)
                     /locus_tag="PC523_002593"
     CDS             complement(13273..13761)
                     /locus_tag="PC523_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HDF2476102.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLYNVKLIKEFV
                     AK"
     gene            complement(13941..15038)
                     /locus_tag="PC523_002594"
     CDS             complement(13941..15038)
                     /locus_tag="PC523_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2476103.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTVIEEKDREWTKKLNDILSRDDFS
                     NEKLDKIKNLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKPFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEESNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILSHVKYNHIEDYVNCLRLT
                     IDGHPDIFSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RVLDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGVEKGIAKGKREERFNIII
                     KLKNKGYNLSEICDIIDDLNKSEVEKIYNQN"
     gene            complement(15411..15581)
                     /locus_tag="PC523_002595"
     CDS             complement(15411..15581)
                     /locus_tag="PC523_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476104.1"
                     /translation="MDSNNDTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            complement(15753..16196)
                     /locus_tag="PC523_002596"
     CDS             complement(15753..16196)
                     /locus_tag="PC523_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2476105.1"
                     /translation="MIEIKTIEINKKGQIDLLMLADPCEEMIDKYLNKGTMYALYDNE
                     ELICIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMIVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
BASE COUNT         5540 a         2782 c         1721 g         6306 t
ORIGIN      
        1 actatttgag aagaataaaa tattgtttaa taaaaattgg gttcaaaata aacttttagt
       61 tcataaagta ctctttttat atgaaatcaa ctgtattttc attattttaa caatgaaaaa
      121 gaagcttatc tctattttta gacagcctct tattattcat ttcctatttt tattagtttt
      181 ctacttttta ttagttttct aagattaaca aactttattt aaaggagtcc ttagatttat
      241 attttgacga tataaatttt caaaaacacc ttgaaagata atacacaatt atacccataa
      301 ttgacaaata atcaatgaaa aataaaatta cttgacatcc tctatcaaag aatacaaatt
      361 gattcacaag aaacatataa tctataatat tatgatgtta ctctcacaca ccaagtaact
      421 ttattttcaa gtaaccttct aaattcataa catctaactt cactcaataa aaatgtcaat
      481 atacatatgt actctatttt ttacttttaa atatctgaca agctttatat actattggaa
      541 gtaagataca aagtaaaaca gcccaatctg gcaaatgtgg catataaaat ttccccttac
      601 cttatacaag ttccttatta ctaaaaattt taatagaaat aaacattgaa gctaacagta
      661 taattaagct taaaaggaac aatacaatat tcactatcat ggatgaatac tgatatataa
      721 atggaacttc aaaattgctg tgacctataa ttaatggtaa tacaaatgat gaagccatga
      781 ttatcaccac aaaaaaggct tttgttgttt catatcctaa tttatattga acaggcatat
      841 aaactccata aataatagaa cttattagta aaacaatcaa aatatatgtt aagtcaaaag
      901 ttcccatttg tggaataagt aaattttcaa tccagtaaac taatgtacaa tagataaata
      961 ccattataaa taaaacatac ttagattgaa ctaagccacg tctaggataa ggagttgaac
     1021 aaagtaatac agatgctttt gaatattgac tctcctttaa taagagatac tggcacagca
     1081 taaattctga aaatataatt gacagtacaa atcccaatat ttgtgcaaac ggaggtaaac
     1141 gccataatat aaataatggt attcctatag caagaaataa tataaacaat acatattttt
     1201 ttacaatcag aacatctttt ttaactaaat gaatcaacat aattactctc tcctttcaat
     1261 atacgcaagc ataatatctt caattgtagg acgctctatc aaaatatctg gcatattttt
     1321 ttgaacttca ttaatttttg aggtaatccc tataaaacca taacctgtaa tttccaaatt
     1381 taaaaataat tttttgattt catcatttag gaacttaata tcccctttta cttttctatg
     1441 ttcttccaag agagtatctt tttcacgttc taatataata cgcccattat caatcataac
     1501 aatcatatct gctattttgt ctaaatcaga tgttatatga gtggaaaaga aaacaccacg
     1561 accacatttc ttcatgtatt ctgttagtat gtcaagtatc tgacttcgga ttaatgggtc
     1621 taagccactt gtaggttcat ccataattaa aaattcagct ttatgtgata atgctaatgc
     1681 taaagcaaat ttcattttca ttccttttga aagtgtatca atcttttgtt ttggattaag
     1741 attaaaatgc ttcatatttt tttgataatc ttcttcgcac caattatcat atgaaggtgc
     1801 aataatactc ttcatttctg ccattgacaa ctcatcataa aaatatgaat catcaaagac
     1861 aaccccaatt ctatttttta attccttctc gtttccattg aagtctttac caaagatttt
     1921 tatttttcca ctatcgcgct ttataacatc caatatcgaa cggattgttg ttgttttccc
     1981 tgctccatta acacctataa atccagttat gcaatcttct ggtaacgcaa agcttatatt
     2041 agataatgaa aaatcggtat aagtcttatt taagttataa acttctaaaa tattttccat
     2101 attaatcctc ctcatataga atatccatca tttcttgtaa ctctatctgt gtaatcccta
     2161 gtgcttttgc atcttttatc atatctgcca ttttttgttc aactatacgt ctcttcgaat
     2221 ctcttaatat ttctaaattt ccagttgaaa taaaggtccc tattccaact tgacgtgtaa
     2281 gaaagccttc attctcaagt tcttcataca ctcgtctaat agttaaaatg ctaactttta
     2341 aatcactagc aaaatttctt atggagggca ttgcatcccc ttcttttagt tcttcagtaa
     2401 atatggcttc tttaatttgc tctttaattt gttgatataa tggcttatct gatgtgttag
     2461 aaattaggac tttcattttt tatcacctcc tttttatcac tttcaatgtg atgttattat
     2521 atacacacta tacacttagt acactttttt tgtcaataca aagatttaca ctcaatataa
     2581 gttaaacttt tcattactaa atttaactat accttatcta ttagtaaagc tgttaaaagc
     2641 atatgttatg cttatataat ttggaaatta attaaaaaag ttatattaaa aataaaattg
     2701 tatacttgaa aagtataaaa ttatgcaaat aagataaaaa ataactttaa taattaaaaa
     2761 gacttatctc aaactagata taagtctctt tatcaatatt aaaatgatta aaatatattc
     2821 aaattcatac aaaaaagtgc cttatggact aagagttcac tttaaggcac ttcttttttt
     2881 atattctact ataaattaaa tttacttttt taactataaa atctatctac aacattggta
     2941 ccataagacc tgctactaca atagaaatca tacaagccat accaccaact accagcttag
     3001 gcataatttt agacatcaaa tatgctcttt cttcttcagt ttctccaaca gcattacata
     3061 cttcatttga aattaaatat gtagttggga atcccaacat ctgacagaag cctactccaa
     3121 aagcaagagg tttagaacca ataattttcc aacctggtaa taccttcatc ataaggaata
     3181 taccaatgat agaggctgca aacataccta caattggtac aaaaagacta attaagtctg
     3241 tcaaagagac ttttgctaaa gaaggaataa cagctgcaaa cacaaccatg ttgataaatc
     3301 ccttgatttt tcctttttcc aaaatatctt ttggcacaat accaatacgt gttacaagaa
     3361 caccaacaac aagacataca atagaataat ttacatgaat aatctctgaa agctcatatc
     3421 ctagataaga gaatagtgct ataaaaaata tacagacatt tgtagagtaa tatttatcat
     3481 atttttcaca gaaagcttgt tttacttttg tccctgtact ctctgcttct ttcttatcag
     3541 tatcaacatt tgctaaatca atgccttttg attttgcttc atgatattct actaaaagac
     3601 ggtttgcctc ttgtagtgct gctcttgaag ctattggtgt accaataaac ttctgaattg
     3661 caaatacaac tgttgctgta gctgctgcaa gtgttaatcc tttttctaat gctgcttgtg
     3721 tcataatagt agtagcaggt aaagctccat ttataactgg tatacttgtt aatactgttg
     3781 attttccaaa caaaggaata cataatgaga cacaaacaat aactgctaca attccaagcc
     3841 atgctgtcaa tacggttctc cattcgtcta taaattgttt aacattcaac attgttccca
     3901 tactaaacat aatcattgga acactccaac tagctgctga ggtcatacct gctttctcaa
     3961 taatatcagc tggtattgct tttgtgacaa agagaattaa aaataaaagt aatgtaacaa
     4021 atacagctga tacttttgct tttgttttac ttgcaatcaa atctccaata gcaaataaaa
     4081 ctaatactac actaaaccat aataacatat cactcataga ataaccccct tattataaat
     4141 atctttcaaa ccaatctaca atagcattta cacgtacgat tctattagat ggtcttccat
     4201 ttctagacaa tccatgtgta tctccgtgga acaaatacat ttcggtatct acaccaagct
     4261 gttttaatgc tgtaaacatt tggaaagtat cccccataaa acatctataa tccctatcac
     4321 tatgcaagaa caatgttggt gttttcacat ttttcacata cttaataggt gaatgattcc
     4381 aaagaacctc ctcatgattc caaggtgtct tatctccaca agtctgcttc ataattctat
     4441 ccccaaagtc aataattaaa gaagctgtaa tcaagttact aatacctctt tgagaagcag
     4501 ctgccttgaa acggtctgta tgaccaatta tccagtttgt catataaccg ccataacttc
     4561 ctcctgcaac acctaagcgt tctgcatcaa ttccaggata tttttcaata caaatatcta
     4621 caaactccat aagatgttca taatcaacat atccccatct ttctaccatt tccaggaatt
     4681 tttcaccata gccatcaccg ccatgagggt tacaataaat cacatacatt ccctttgatg
     4741 ttacacactg ctttaggtgt gtaaacatat agccatatgc ccatttaggt ccaccatgaa
     4801 tgaacaatac agcaggatat ttttcgcctt ctttagcatc tataggtggt ataacaaaac
     4861 catcaacttc ttctttattt tttgctataa acgtcaattt ttcaggcaat gaaaccttat
     4921 gagtgtctaa atagggttta gaaaaagcag tcattttttt cattgtacct gtagtcatat
     4981 caatgctata tatttctgcc aactcataat ctttcaacat tactgtatac atagtgttat
     5041 cataaatatc aaaagatgta aaagcatcta cttttgtgat aatacgtata ttacctgtat
     5101 ttgtatcaaa ctcatcaaac tctgtcatag ttctgcaaat ccttgcacca atgaatttat
     5161 ctcctataac tttattacta cgtccaccac catatactgc atctacatcc agtaatgtag
     5221 catacatacc atctagacta gcaatcatac ttatttcact agtgctcaaa tcaagtataa
     5281 aaatatcatg attttgtgta atagaacggt ctaatgttga agcttgcaac attactttat
     5341 tttcgccaat atattttgcc aaacttattt ggtattttcc ttgtggtaca agttcctttg
     5401 tttcaccact ttctatatgg tactcccaaa gcccagaaaa aggagttgca caatcttcat
     5461 aaatctgacc aacatataaa attctttttt tatcagaaga aacatctata cccgccactt
     5521 cacaaaattt tggtgtaatg gcttttgttg tattttcacc ttcttgatat agttctaatg
     5581 ttacacgtct tctttgagaa aagttttctc catctcttat aaaaggttta tctgtaaatg
     5641 tccaatagtc tataccctct actgccctat ctggctctga ttttttagaa tctgttggat
     5701 tttttgttgt caataaccac aatttatttc caagtgatac aggtacgcta caagcttttg
     5761 ggatagaaaa tgcttttttt gcttctcctc cttctataga aatagtgtaa aaatctgttg
     5821 tacctgcttt tggcttattt cttttagatg taaaaagtag ttctgtatta gaaatccaac
     5881 taaatgatgt tacatctttt gtttgagtca tatgtatttg tttttctgtt ttgatgtcca
     5941 tcacatataa gtcatgttca tagcagtctt tttcctcatt gatatttgat ataatataag
     6001 caatatgctt tccatcaggt gaacaagaaa tatcatgagg gaatatatat tcaaaaatat
     6061 cagaattttc aatctttatt ttttccattt ttatattctc ctttctctac tgtcctatgt
     6121 tatctttttt tatgtacaca gaaaaccaat ctagcatttc aactaatctt tgtagctttt
     6181 gttttggtct accagaacga gacagttcat ggttttcccc ttcaaataga caaagttttg
     6241 taggacatcc atgaattttc aatgctgaaa acatctggaa tgcttcttgc atatagcatc
     6301 tatagtcttt ttcaccatgt aaaaataaag tcggtgtaac acatttatca gcataagcaa
     6361 gaggtgactg tttccattga agctcgtggt tttccatatg tgaagcacct gtttgtcctt
     6421 tgcagaaata ataaccaatg tctgttgtac cctccatgcc actccagttt gcaatagagc
     6481 gctgagcaca agcaacagca aaacggtttg tatgaccaat tatccaattc gtcataaatc
     6541 caccataact tccaccttct acggccaatt ttgttttatc aatgtctgta tatttttcaa
     6601 gtacagtatc tgtaaattcc ataaaatcta tataatcaat atctccaaac tgtttcctaa
     6661 tatctgcaaa ggcattgcca cgaccttcag acccccttgg gttacagaag aatacaaagt
     6721 aacctgcact tgcaaatacc tgcatctcat gcataaaata tggtccatat gtcaatctcg
     6781 gtcctccatg aatcactaat acagctggat attttttccc aacttcataa tctactggtt
     6841 tatatacata gccttgtatt tcccatttag aacttccttt tgactctatg tattctggtt
     6901 cagaaatttt atattcctct aaaatatggt catttaaatg tgttttcttg cacagtgttc
     6961 cattctctac cgtataaact tctggtaatc tatctcctaa aaaagctaca catacaatat
     7021 tgccatcttt tacatctata aaatcaactg aacctgtctg tgttactttt tcttgttttc
     7081 ctgttggaat atgtattctt tcaataatac aatccatatc tactgtagaa gtgtaataaa
     7141 tatattctcc gtctaccttt gttgtctgac caacacccat tttaacatca gatgttactg
     7201 tatttccaat acaatgatgt tgatgctctg aaagaagttt catttctttt gtatcaatat
     7261 caatagtata aaaatctccg ttttctccat ttccataaga taaagcatca gttaggttta
     7321 gtatcacttg attttgatat aattcaaaac cttttacaat atacttatct ttttctaata
     7381 tacaaacatt tgtgtttgtg tttgtatcaa atacatagac tccatttttc aaacctttta
     7441 catctttaaa ctcttgccct acatataaga tttttccatc acttattttc atacctacaa
     7501 cattaaacat aggtgctgta attgccttca actcatttat attactgtca tatagatata
     7561 agcgttttct ttttctacta acataccctt ccccattaac agcataagga atttctttga
     7621 aatgttcata gtgtctgttg ttataagcat ttattttttt ctgttgttct tcttctgaca
     7681 atccctctac aataggtcgg tcgttatcat aagaatcaat aactgcatac aaaccttttc
     7741 ctatagggtt caatttacca cctttgatag ggagtctaaa aagttcttct gcttctccac
     7801 catttaaaga taaagcatag tagctcatac attgtttacc atctttgaca gcttctttta
     7861 ctttctcatt tcgtaaagca gggaacaaga tagtattttc atcttcccag gcatatgctc
     7921 caacagagtt tatagacgtt atttgtttta attctccact tttattgttt agtatataaa
     7981 cttggcttgt gtaagcattc tctttgatat cagctttttt tacaataaat gctgtatatc
     8041 ctccatctgg tgatatctgg caatcactta taaattgata ttccaaaaat gtttcacttg
     8101 taaatagctt tttcatagtg ttttctatcc tttctttagt aatttttttc aaacttctta
     8161 ccacctcttt cattttttat tttaaattta aaataaaaaa ttttaaattt aaaataaaaa
     8221 attttataaa ggttattttt ttaggaagcc ttatggcttc cttataatat ttgtaattta
     8281 aagtatattt aattcatgct taattggtac ttaatccata cttaatccat acttaatcta
     8341 tatttaattt atacttagac ttatttacac tgtatttaga ttatgtttaa actacattta
     8401 gattatactt agactacatt tagcaactta atagataagt atatatagta acttcaaaac
     8461 ttattattca tcttcacaga atcttttaat agttccttga atatgatttc ttaatttatc
     8521 tactgctttt tttgtatctc tgtcttcaat gaattttaat atttcaaaat ggtcttgtac
     8581 tgttccttca taattacgat tttttaatgg tcgaattaaa tagttcacta ctataaatct
     8641 tccttgtata ccaacatata catcatttaa aatctcatta tttgcccctt ctacaatggc
     8701 tttatgtaat tccaaatctg ctagtgcaag ttctacagat ttttcctgac gatagtactc
     8761 ctcacattta tcagagtatc tttttatcac atctaacttt tgttgaaaaa cgtcttcctt
     8821 ttcttgcatc attttttcta aagcgtatgt ttcaatcagt gttctcgttt cacaaacttg
     8881 ttttaccata tcatattcat actctggtac gtacacttta ccatttgtca taatagtaag
     8941 cacaccatct ttatttaaca tttttactgc ttgtacaact ggtgttctgc ttacttcaaa
     9001 tttttcacaa aggtcattta gtaatatttt ttgacctaca gagtattcac cacgaataag
     9061 actttcaata atcttatgat ataaaatttc atctaaattt gactttactt tcactactct
     9121 caccttattt ctattctttt gccatattat attgaactac ttgaccttaa ttacttttag
     9181 taacgttttt aaagtagaaa ttcttatttg atagtacttt tggactatag attaaattat
     9241 tgtactgcaa gtcaagttat tgtactacag tttaaaccat tgtactataa gtcatgctat
     9301 tgtactatgg attaaatcat tgtactacaa aacaagctat tgtactatag cttaaaccat
     9361 catactgcaa atcacgttat tgtactatag tttgaaccat tgtactacaa gtcatgctat
     9421 tgtactatgg attaaatcat tgtactgcca attaagctat tgtactacaa attaaactat
     9481 aaatgcttac tatctcttaa taattggaat aatatgttac aaaaagtata acaccttttt
     9541 atttttttcg caataaataa aaaatttaaa attttttatt ttttattttc ttgtattata
     9601 agtttgaaac ataaaagatt aacactaata gaattgattt agctagttga aaaaaagttg
     9661 aaaaaaatag gtaactttta tgaaaattat aatgataagc atttttttga ggtaatagca
     9721 agttataaat gataaaatta aaaaccactt ttagttttat ggactatccc cctattaggg
     9781 aacaattaag atatgtaaaa atagttataa ataactagtt aaactatacc cactatttta
     9841 tatggttaaa atagtgggta tagttcaaac taagaatgat taagaaatta gcattaaata
     9901 ctataagtaa aaacataatt gtataaaaaa catatttttt tgctacaaac aattatttcc
     9961 ataacatttt atatacttct ctaaaatctt ctaaacttaa taaatattca attaaaggga
    10021 cataattacc tatactataa gaatttaagg agtttctata caagtcctca ctacctcaag
    10081 gtacaatttt tataggaaga ttatttttct aacaagtatc caacaagctc cacattatct
    10141 acatctactt caacataatc tgtttgatat ttgtgtatat ctgacataga aatttcttta
    10201 ataagtttac catcttgaat aatgccaata gtatccgcaa tttattctac ctctgaaaga
    10261 atatgactag aagtaaatat agttgttata taatctgtat ttaacttatt gaataattct
    10321 cgcatttctc ttattccctc tgggtcgagt gcatttatag attcatcaag aatcaataat
    10381 tctggcttag taagaattgc tctagctatt aaaagacatt gttttatacc taaaaagtat
    10441 tttgaaactt ttttgcttga ttgttttgac aatccgacac atttcaatat ctctgatata
    10501 tgttctttgt tataatagcc catatattca caatgtaagt caagattctg tttatcagtc
    10561 aaattctcat agaaataagt attttcaata atagtactaa tcttttttaa catttcaaaa
    10621 tcagtttctt ctaatttttt accgaatata attatttctc cagaatttga cttagttagg
    10681 tttaacaaca ttttcatcac tgtactttta cctgctctat tttgtacgac aaatccgtaa
    10741 atgacttcct ttcttacatg aatattaatg ttattaataa cagctttatc tttatacatt
    10801 ttatttaatt gagtaggctc tatggtataa ttcataatac ttcttttcta aaaatagtat
    10861 acaaaataaa gttatctttt ttagctttca aatctaactt ttttctaatt tttcaatttt
    10921 actgtaaaaa cggtttttat attaggtcta ctatttatat taaatagata taaaaactag
    10981 tcaatctcat agatgtaaat aattaaatta gatacaaaaa catagttaac cttattactc
    11041 caaatcatta agttaaatac aaaatacagt tagacttatt actctgaatt attaggttaa
    11101 atacaaaata ccattagaac tttactctaa aacgttaaat taaatacaaa atgccattag
    11161 aattatttct ccgaatcgtt aagctaaata caaaatacta ttagaattat ttctccaaat
    11221 aattaaattt ttaaaaaatt ctttatctat tatctaaaat ttattgtcaa gactctaaag
    11281 ggtttagaca tcataatttt catattatct tgtatgtaat tatcaaatat agtaatcaaa
    11341 atagtaccat ctatctttta aaatactttt taacttttac taaaaaatta ttttatacat
    11401 aaaaaagaaa ccacatccca gttttataca gaacatagtt tcttcaaaaa taatcaaatt
    11461 tggattttaa ataattaaaa acaaactttt atttgttttt aattttccaa ttttatagta
    11521 gataatttag ttaattagtt cttaaatgtt tatcttcttt agtaaatttt tttcttatta
    11581 agaaatatgc aactattaca acaagttcta tagctaaaaa tataccataa gtagctatag
    11641 aacctatgct atctatgcca tttctaaaga aaattaaagc aattcctgct gctataatac
    11701 ctatagcatt tttccctata gaattttgac caaaaccata aaaagaaggg aaatttcctt
    11761 ttatttcaat gaatgtcaag attaatccaa tagctataaa tagtactcct acaatttcca
    11821 taatcatact aggcattttg aacactcctt attaataatt aactactact ataaaaatat
    11881 ttattatttt tataattata ttatatatgc gattagatgt ttttggaagt atatttggaa
    11941 gtaatttcat atttatttaa cttaattttt ttgtgataaa atatttctaa ttttcatata
    12001 gatattatta taaattttct agtttatgga gctattcatt ctataattca caaatatata
    12061 ttttttaatc tattaaacta ttcattttct gcttataagc atggacttat tatctataag
    12121 cttggttttt catctataag tcatttattc tttaatctat gagcttgatt tttcatctat
    12181 aagtcattta ttttttaatc tatgagcttg gtttttcatc tataagtcat tcatttttta
    12241 atctatgagc ttggtttttc atctataagt catttatttt ttaatctatg agcttgattt
    12301 ttcatctata aatcattcat tttttaatct ataagcttgg tttttattta taagttattt
    12361 attctctaat ctataaggtt gcttcttcat ctataaatca ttcattcttt aatccattag
    12421 cttggttttt catctattaa tcatttattc tctaatttat aagcttggtt ttttacctat
    12481 aattaattca ttctctaata cataaacttg aattttttat ctattaattc attctctaat
    12541 gcataagcaa tacatgaaat ttaaatatca ttttcttcaa taaattcaaa taactcttct
    12601 acagttgtat caaatacctt agcaatatcc attgccagtt ttaaagaagg attgtagcga
    12661 ccattttcta aatgtacaat agtttcacgt cttactccta ctgactttgc aagttcacct
    12721 tgtgtcataa ggttcttttc tctataggat ttaatttttg tctttagtaa tactgacacc
    12781 taattgtata tcccctttct gtcaaaatat ataaataaaa ttaacttcaa caaggattga
    12841 ataaacaaaa tgactaataa agacattcct atatttaaat ttgacacatt tatgtccaaa
    12901 gttacaagaa atatagatac tatagctaca gaaaaaaaca ataattttat tgcaatatca
    12961 ttaactcttg atagtataca ttttgatgcc tcatcaatta aatcatcata ctttttatta
    13021 tagacccact gagctcccat agctaatacc caaataatta taggaagata ttcaatacta
    13081 cttcctttaa aaaaatactt atttactaat gtagctccaa tacaataaga aattcccaat
    13141 atttctgaaa taagtgttga tttaatacca gtttgttgtt tagacatata aaatccccct
    13201 taaaaagtta tatatttata acacctcttg ttataaatat ataacttttt tgaataaatt
    13261 tcaataaaaa tattattttg caacaaattc ttttattaat tttacattat atagaggaac
    13321 tttagatgtc actccttgca atgtcaaatc ttctatatta acttcattta attttgaaca
    13381 cagttctaag tcaaatattc tttcccaact taccttcttt tcaatttgtt catcttcata
    13441 aagctctaca gtaatatcca ttaaaggtaa atgccataaa tcaaattctg ataataacac
    13501 attatcttca cttatttcta tttcaagtaa aacacctttt tttccttttt ctaaataacc
    13561 actacatctt aaatcaggtc tcttcgtcca aacccatatc atactatcat caaattcata
    13621 tattctattt gtcatttgac tatacatcca tttgtatgga atatcaaagt cttctaatat
    13681 aaaagaataa tcatttgata gatatttatt gtttttattg ttgttccaaa attctaaagt
    13741 ctgtatagta taaaatttca tatataaatc aatccctttg tttttctata agaaggagat
    13801 ggttcaaact tttattttaa atcatctcct tagcttatat atctctttgt atctactata
    13861 gttaaataac aaggttttat attaatttaa atttaaaaga ggaagctttg tatttatcta
    13921 gtgccatctc aattatagta ttagttttga ttatagattt tttcaacctc tgatttattc
    13981 aaatcatcta taatatcgca tatttcactc aaattgtatc ctttattttt taattttatg
    14041 atgatattaa atctctcttc tcttttccct tttgcaatac ctttttcaac accttcttga
    14101 aaaatttctt ctttaaaatt tctactttta ttcataatat acgcacctct ctttaaattt
    14161 aataaatagt cctcattact gcctaattct tttaatctat caagtactct tttaataaca
    14221 taatccgttt tagatagtat tctaatatcg atatgagaag gattatcaat aagtttaccc
    14281 catattagaa ctctatttgc tatacaactg gatttacgaa tttttggaag ttctataaaa
    14341 tataatgcag ttttactaga gaaaatatct ggatgcccat ctatcgttaa tctcaggcaa
    14401 tttacataat cttctatatg attatatttt acatgactta gtatatgtat tccaacagtt
    14461 tttccaattt ctttatattc tttaccttct tctaactttc taccatgtac acgactaaga
    14521 taatattcag ctctttccaa ataatcctct tcaagagctc tttgcatctc aatatttata
    14581 tgattacttt cttcatcttc agccaaaata tcaagtctag gtcttttctc accaatgtaa
    14641 ttaattgttg ggtcggtttc aaccttaatt attcttttta tttgagtgaa cggtttgata
    14701 ttcctattag catatggagc atctttgaga atatcattta taaaatttgc aagtaaatcg
    14761 taatcattat ttaaagtaaa taatgattta aatacctcat catactcagg cttagcatac
    14821 ccatcttcag ttacaacttc tccaacattt tcactagtca atatttcttt tggaattaaa
    14881 ttcttgattt tatctaattt ttcattagaa aaatcatctc tactaagtat gtcatttaat
    14941 tttttagtcc attctctgtc cttttcttca attacagttc tcttatactc ttttgatgtt
    15001 ttaataatct tagatttttc ttctaatttt tctttcatat caacacctct tttttattat
    15061 tacccatttc ttcataccaa agtctgcact tctaagtaaa atttgcatta ttgaaataag
    15121 aacatgataa actcatatat cattactaaa gttaagactt taaagagaat cacttagatt
    15181 acaatagatt ttagataact ttctttttaa aactattgtt gataaatgaa tgaatataaa
    15241 ggtatgtatt aaaatagaga taataatctt tttcattgtt taagtaatga aaatatagtt
    15301 tatttaatac aaaaaagagt gccttataaa ctaaaagttc acttcgagac actcccttac
    15361 attttgtttc ttaaacttca caatattaca cacttattta ttaatttaat ctatgcatac
    15421 ttaacccaat aataaagtac atatatccaa ctcattaagc catgaaaaat agcccatgga
    15481 atagatttcc atgcagtata agaaataatc attgctaaac aagaaccaaa agaaataccc
    15541 tttttaacag ttgattttat agtatcatta tttgaatcca taattaatcc tccaaaattc
    15601 aaaataaaca agttattata tatattattt gaatctaata caatataaca gcttctaaat
    15661 aaattatact cttattatac ataatttata aattatagtt ccaataatta tatgaataaa
    15721 ttatttatat taacctaaaa atattatcct atctaaaagt tatatgatag atacaacatg
    15781 tcaacacatt gtaattctcc ctcaaagatt ggttctggat aattatctac aaaaaagttt
    15841 ttaattttat gtgaataaac aaatccaaac ttctcataaa aaggaactcc gctttcagat
    15901 gtaccaacaa tcattgatga atatttttta ctataaacat ctaaaagatg ataaatcatc
    15961 tttgaggcat aacccatatt ttgaaaatgc tcatatgtag caatgttttt taattcacat
    16021 atttctttgg aaatttcatt tacaactgct atacaaataa gttcttcatt atcatacaat
    16081 gcgtacatag ttcctttatt aagatattta tcaatcattt cctcacatgg gtcggctaac
    16141 attaaaaggt caatttgacc ttttttattt atttctatag tttttatttc tatcataagt
    16201 tctccaccct cactttattc aaaccttaat ttagcaaact tcttacagta cttatttaat
    16261 tcattttgat atatattttt acttttttaa aatatagtga taattcccta gaatattaat
    16321 actaataata aattataata tcagcacta
//
LOCUS       DAMJVM010000074        16257 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.077, whole genome shotgun sequence.
ACCESSION   DAMJVM010000074 DAMJVM010000000
VERSION     DAMJVM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16257
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.077"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            172..318
                     /locus_tag="PC523_002597"
                     /pseudo
     CDS             172..318
                     /locus_tag="PC523_002597"
                     /inference="COORDINATES: protein motif:HMM:NF013180.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            408..506
                     /locus_tag="PC523_002598"
     CDS             408..506
                     /locus_tag="PC523_002598"
                     /inference="COORDINATES: protein motif:HMM:NF013180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2476106.1"
                     /translation="MSVSIGISLYSKYGEQFYKLYRHADSALYRVK"
     gene            704..2524
                     /locus_tag="PC523_002599"
     CDS             704..2524
                     /locus_tag="PC523_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2476107.1"
                     /translation="MKKKYDFLIYAAIWLVAIIIFIFSLYHSIEHIKIINYTGIISSE
                     TQKVTKQELNNKRNDKLISQLDSILIELRTGNGKHRLQRCDNKEFQEKLIEMDAMWDS
                     MKKEIRKVRNGGSGDKLYKLSEKYSSLSNQVVFISEKHSDIKLHFFATVLFIYLIIST
                     ISLLIWEYYNKRKFDKIFYTDNLTKIKNQVAFENRAIEILSHAYNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLVIVANVLAKTFNTKETCARVGSDNFVILAKYRESLLYEISKT
                     LTDAIISELNMNVTQTISFCIGAYLVEIEKSNYKYINSIMDKANIAHKVSKMRGISST
                     VWYNENLLKQLQMENSIYNHMYKALENEEFQMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDQFIPLFEKSGFIIELDFYMLKKACSFIKKTFMKKNKYTYPIAVNFSRVTIY
                     QNDFYQRFLDTVREYEIPFKYIEIEVTESAFNEISEPVISILDKLEKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKEKFNRAKVYSIIKCIIEMSHSIDMSIVCEGIET
                     KKDLEFLKTVKCDLGQGFYFSKPIEEKEFFNKYVMDKQYN"
     gene            complement(2609..3190)
                     /locus_tag="PC523_002600"
     CDS             complement(2609..3190)
                     /locus_tag="PC523_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476108.1"
                     /translation="MSYLLKINQFERSYDIGIFKNEQSIHKFIEKIPFVKKDSSNFNY
                     TNYYMEFEDIPEYYEVNFNNYLYIITKFSFIPDGTEIFFTWDEIHCWDEKVATDKTFI
                     NGETLVDSYSFPNNEVKNYIQKRESLYRETKKYYKEKGLKIKRHALGSEDGEYVELVD
                     DSILYLLDVHAVEIWEQSKNIEEFLYKYKKYFE"
     gene            4376..5071
                     /gene="cas6"
                     /locus_tag="PC523_002601"
     CDS             4376..5071
                     /gene="cas6"
                     /locus_tag="PC523_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF2476109.1"
                     /translation="MIKCLEMSVKVYLLENISLDNTSKEIRKLIDDTLIIEEKYKEFH
                     NKAGFKNYSFNLLYPLEKDKVYKKDCIYTFIIRTVDSELGMYFMNNLKNQYSDKIKVI
                     TMSKKVIPKKHIESIYSVTPIIAKFEDGYWRSNKTVEDLEKRIRENLIKKYNEFTNSK
                     IDEDFELFQVIKFINRKPISTKYKNISLLGDKISFKVSENDMAQEIANFALGVGVGEI
                     NSRGFGFVNYRWL"
     gene            5062..6822
                     /locus_tag="PC523_002602"
     CDS             5062..6822
                     /locus_tag="PC523_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cse4"
                     /protein_id="HDF2476110.1"
                     /translation="MVIGGENLLKDCLEIFEKEYKSLGNRLITDSYVLSYGTYILVKN
                     FSNIDILEVSKNNVDSTEDIYHKFCELDYLSKLVSIDKSVDSKKIIHSNNYLSFFVKK
                     ENLRPDKNNNVKLTEEIIDNYYDALLNPRIKYKSDKRKKEIYDSIENKYGKVDENKLN
                     KIRLWIKTNIFDLVESVKKDKNYLKIFFDEKIELYNDENQKYLYPNIYNKNDFNVIID
                     GKNYGLPNDNMQLNEKKPFLKLKTRIRNSTPYLISDDEVLLQKAFFDYLMNKAANGEE
                     VVYISSEKGIHRKTDIPNEFSGYILRIKKGKELEIHDFDTVVNLNTKIKRINVECIIP
                     IEYERHNLKPNLEIGYINNLIKLEKTIDTIFFNKFMCNSYFKEIKNINLSDLVLRDIL
                     IRHREGLYTWFYKGRDNIVKQTFSNYSMGIIENSIKNNNYIRAKEQYNLRIAIINYFH
                     GGSNMADKIKVISESLFEKINSIKTKCIQSDEEYYFAVGQLASYFISKNKSKNKNHSL
                     INPILNAQNRKKIMLEIEKLFKKYNYDISYDNNRRFKNLYSMIVGYEVEEKKVNRDLL
                     IGGYLYYSLIYTKSEENENE"
     gene            6809..7804
                     /locus_tag="PC523_002603"
     CDS             6809..7804
                     /locus_tag="PC523_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I CRISPR-associated protein Cas7"
                     /protein_id="HDF2476111.1"
                     /translation="MKMNRRVYGVLGIKSIMSNWNADFSGYPKTTSTGEVFGSDKALK
                     FPMKKMWENEGEKVLYVKSLKIDESKKGEIKIRPKSLKERYEELFDIEELKLEKNQQK
                     VLENLFKAIDVKNFGATFAEEGNNIGIMGAVQIGQGFNKYEDINVEEQQILSPFRDPR
                     AKDKKKSTNEEEKEDANSTTLGKKIVSDEAHYFYPFSINPKAYEVFVNMGVTDGYTKN
                     DYKKFKEAALVSATAFNTNSKLGCENEFALFVETDEELYLPDLAQYITFEKREKSKDL
                     ICINIIHLIAELKDDIKSVEIYYNPYKIEIKPFKDEILPKIDKVKFMNIFTRKEV"
     gene            7806..8546
                     /gene="cas5b"
                     /locus_tag="PC523_002604"
     CDS             7806..8546
                     /gene="cas5b"
                     /locus_tag="PC523_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HDF2476112.1"
                     /translation="MEVLKFNLSGKTAFFKKPDVNSIIYFTYGNIHKIALMGILGACL
                     GLGGYNQQSKEDEYPEFYSLLKDIKMSIVPKHEFGYINKKVHIFNNSVGYASKEEGGN
                     LIIKEQWLENVSWDIYIQLEDSTTSRELKRRFLSNKFVYIPYLGKNDHIANISEIEII
                     TDFYKTEDINRVDSFFIREDFEFEKSYFDDNDDYEEPFKYQEKLPYRLHRMTNQYILE
                     TIIFTNMSVKLINKKEVYQVKGLNLIFI"
     gene            8570..11128
                     /locus_tag="PC523_002605"
     CDS             8570..11128
                     /locus_tag="PC523_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF2476113.1"
                     /translation="MYFKKVELFDISNNINNCKNIYAHINKDRKPETLKEHSDLSYSY
                     LMKLCVKKDLSSCLERIENKFFKKDSFLSKNFYREIILNMVYMHDIGKINSSFQFVKM
                     QNKNFKKISLRYTKHSFISSMLYFDYYFEKLKKYDFEEKKILSLFLMINTYTISKHHG
                     VLKSFKEFLFLFKDEFINIKENFKDLFKNNYNLELKSVPKTIEKMVRGVEGYLDDSHI
                     KDNLAIDLYIYSKLMFSIVTSCDFYATSEYNSGNSIDGFGTIDNYNKYYDVLKKSEIY
                     KGMKKYREYLNNNSECPYKENDINRLRTEMSIEAEDAITHNTRKRIFYLEAPTGSGKT
                     VTSINLALKSIELNQSLKKIFYIFPFNTLVEQTKDVFINEIFNSVKDIQKDVVVINSI
                     TPIQTEDKEEDNKNVEYCVTKQEIDYDKSLLNRQFLHYPIVLTTNIGFFNYLFGVSRE
                     ECFPLIHMINSVIILDEIQNYKNEIWKEIILFLEKYADILNIKFIIMSATLPRLNKLG
                     CNDDNFAYLVKNREKFFKNHLFKDRVNISFEMLNSEINDIEEISEKVIEEAEIYNKIL
                     IEFIKKSSALEFYKNIKEKLKYKLEDVFLLTGDDNKLERKKIINKIKDMNSVILVATQ
                     VVEAGVDIDMDLGFKDISTIDSDEQFLGRINRSCKKLNSKVYFFNLDDASKIYKKDVR
                     KERHLTLNEESNRKIILDKDFEKYYDKIIARIEENKHKHNDKNIEVFIKDIVGNLNFT
                     EVKKWMALIPDLKEYTIFLNTIVKDESGNMIVGSDVWYEYISLLKNDNVEYSEKRVKM
                     SEIMEKLDYFTYKVQKFDNSFNDLVGDIFYIDDGSKYFTEGKFDRSKFNQNEFL"
     repeat_region   11253..13916
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=11318..11347
                     /rpt_unit_seq="gttaaacagtaacatgagatgtatttaaat"
     gene            14001..14495
                     /locus_tag="PC523_002606"
     CDS             14001..14495
                     /locus_tag="PC523_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HDF2476114.1"
                     /translation="MKITGTLINYYFHCKRQCWLLGNRINLEENSEDVKIGRLLHELK
                     EEKSKYKEIAIENIKIDKLTREYLVEVKKSDADIEAVKWQVLLYLKKLKEKGIVRKGK
                     IEFIEKNKTNKKIVFVDLSEVSEEQLISIEREIVNLINSDKAPCPDLKKGCKKCAYYE
                     YCYI"
     gene            14530..15528
                     /gene="cas1b"
                     /locus_tag="PC523_002607"
     CDS             14530..15528
                     /gene="cas1b"
                     /locus_tag="PC523_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HDF2476115.1"
                     /translation="MGKTKYITSMGELTRKDNSICFRKNGRNVYIPVENTSEIFCLNE
                     VSLNSKLLDFLSRANIVVHFFNYYGGYSGTFYPKEYLISGKVTVAQVKTFEDRREEIA
                     KSIVNGIRINAINTLSHYYKHGNSEIQPLLSHLKKDSINKLEKSNNIKEILSVEGDIW
                     QTFYTSFKYILREEFIFNKRVKRPPDNPLNAMISFGNSILYSKTVTAIYNTHLNQSVS
                     FLHEPSESRFSLSLDLSENFKHVITFKTIFDLVNNRKINIEKHFDKNLNYCVLNDEGK
                     QIFINSLEERLESVKEHPKLKRKVSLKTCIKLDAYKMLKCIMEEKSFIPYNIKDGL"
     gene            15739..16029
                     /gene="cas2"
                     /locus_tag="PC523_002608"
     CDS             15739..16029
                     /gene="cas2"
                     /locus_tag="PC523_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HDF2476116.1"
                     /translation="MKKKNNLNYNYVFVVYDVGEKRVSKVFKVCKKYLSHFQKSVFRG
                     EITPSKIMSLKRDLENIIDKDNDFVCIIKLMNSNTFEEEILGNNSNCENMIL"
BASE COUNT         6725 a         1441 c         2411 g         5680 t
ORIGIN      
        1 attattgtat ttattgtata aacataggcg taattcaagt aaaatttcaa aaaaaaggaa
       61 tatatttcca taatatgata aaataaattc aaagagttaa attctagtca aatattactt
      121 taattattta ttaaaataga ttagatattc catattcaat accattgagt tgatgtgtat
      181 aattttaaat atgtaaagga ttctatgggg tatgtatttg gagataaaat attaattgat
      241 ttagctgaat gtttaaaaag atattttaga gaaacggaca ctgttggtcg tgtaggaggt
      301 gatgagttta taatatagtg aagaaagaga aagtgtaaaa aataaaggct ttgataatat
      361 taaaaaggct ggacaataag tttacgtatg atgaatgata ttttgagata agcgtaagta
      421 taggaatttc tttatattca aaatatggag aacagtttta taagttatat cgtcatgcag
      481 atagtgctct atatagagta aaataagaag gaaaaaacac gtattgtatg tttttctatc
      541 taaaaataaa atctatatta taaaggattg ttctatctta ggtatataaa tgattgagat
      601 atttaagata aatacatatt actgcaaagc attgataatt atttttgatg agagattgat
      661 tatttgcaaa ttatatagat acattataat tgagggaata ataatgaaga aaaaatatga
      721 ttttttaatt tatgctgcta tttggctagt tgctattatt atttttatat tttctctata
      781 ccatagtata gagcatatta aaattataaa ttatactggt attattagca gtgaaactca
      841 aaaagttact aaacaagaat taaataataa aagaaatgac aaattgataa gtcaactaga
      901 tagtatttta atagaactaa ggacaggcaa tgggaaacat aggttgcaaa gatgtgataa
      961 taaagaattt caagaaaaat taattgaaat ggatgctatg tgggattcta tgaaaaaaga
     1021 aattagaaaa gttagaaatg gtggttcagg tgataaatta tacaaattga gcgaaaagta
     1081 ttcttcgttg tctaatcaag ttgtttttat atctgaaaaa cattctgata taaaactgca
     1141 tttttttgcc actgtattat ttatttatct tataatttca acaataagtc tcttgatttg
     1201 ggaatattac aataaaagaa aatttgacaa aatattttat actgataatt tgacaaaaat
     1261 aaaaaaccaa gttgcatttg aaaatagagc tattgaaatt ttatctcatg cgtataataa
     1321 ggagtatgtg ctactaaaca ttgacattga taattttaaa tatattaatg atacacatgg
     1381 atatgaatat ggtgataaag ttttagtaat agttgcaaat gtacttgcca aaacttttaa
     1441 tactaaagaa acttgtgcaa gagttggttc agataatttt gtaatacttg caaaatatag
     1501 agaatctctc ttatatgaga ttagtaaaac tctaactgat gcaataatat ccgaattaaa
     1561 tatgaatgtg acacaaacta tatctttttg tataggagca tatttagttg aaattgaaaa
     1621 atcaaattat aaatatataa attctataat ggataaggca aatattgcac ataaagtaag
     1681 taaaatgaga ggaatttcat ctacagtgtg gtataatgaa aatctattaa agcaattaca
     1741 aatggaaaac agtatctata atcatatgta taaagcacta gagaatgaag aattccagat
     1801 gtatttgcaa ccaaaatttc aaatatcttc tttaaatgtt gtaagtgcag aagctcttgt
     1861 acgatggttt tctccagaac ttggtttttt gtcaccagat caattcattc cactatttga
     1921 aaaaagcgga tttattattg aattagactt ttatatgtta aagaaagctt gttcattcat
     1981 aaaaaaaaca tttatgaaaa aaaataaata tacttatccc atagcagtca atttttcaag
     2041 agttactatt tatcaaaatg atttctacca aaggtttttg gatactgtta gagaatatga
     2101 aattcctttt aagtatatag aaatagaggt aactgaaagt gcttttaatg aaatatctga
     2161 gccagttatt tcaattttag ataagcttga aaagttaggt ttcttaattt ctatggatga
     2221 ttttggctca ggatactcgt cattaagcct tttatgttct ttatcgatta atggattaaa
     2281 gttagataaa agcttactta aagagaaatt taatagagcg aaagtatata gtattattaa
     2341 gtgtattatc gaaatgtcac atagtataga tatgtctatt gtatgtgaag gtattgaaac
     2401 taaaaaagat ttagagtttt taaagacagt taagtgtgat ctagggcaag gattttattt
     2461 ttctaaacct atagaagaaa aagaattttt taataaatat gtaatggaca aacaatataa
     2521 ttagttttat tgttaatctt atgcattgag ttattttata tcttattaaa tagctcaatg
     2581 catagttatg caggtcaaaa aacataaatc attcaaaata tttcttatat ttatataaaa
     2641 attcttctat attttttgat tgttcccata tttcaactgc atgtacatca agtaaataaa
     2701 gtatggaatc atccacaagt tcaacatatt cgccatcttc tgaaccaagt gcatgtcttt
     2761 tgattttcaa tcctttttct ttatagtatt tttttgtttc tctatatagt gattctcgtt
     2821 tttgtatata gttttttact tcattatttg gaaaactata tgaatctacc aatgtctcac
     2881 catttataaa agttttatca gtagcaactt tttcatccca gcaatgtatt tcatcccaag
     2941 taaaaaatat ctcagttcca tctggaataa atgaaaattt agtaataata tacaaataat
     3001 tattgaaatt tacttcataa tattcaggaa tatcttcaaa ttccatatag taatttgtat
     3061 aattaaagtt actactatct ttttttacaa atggtatttt ctcaataaat ttatgtatag
     3121 attgttcatt tttaaaaatt cctatatcat agcttctttc aaattgatta atttttaata
     3181 aatatgacat atattctcct tatttgttga aatataattt ttataactct actaatttta
     3241 aacattttta aataaatatt tgtatatacc ttatataata ttatagtaat tagttttaaa
     3301 tcaatagaat agtatgtatt tatctaatgc ggcaatataa tttaaagctg gtgttgtaga
     3361 aaaactaaga gtgaatatag caaagagaag caatagtatt ttaaactttg tctctcttat
     3421 tttttgaaaa ctaacattaa aacataaggt ataaataata ttatcatata aatgaaaaca
     3481 aatttgaatt tttctctggt cgatgtatat agtaagtttg ttgataggag aaaaattatt
     3541 attgataata tacatgcata gaaaggaaat ggtatataat attttcttaa gtttcttttt
     3601 taggcatttc tattggcatt ttaggctata gtactaaata cattaaccaa caaaataatg
     3661 aaataaatat atatatagaa aacttaattt tcgtgggaag aaaaaaagca ataatagcaa
     3721 gtattattag agaataacga ataatacgag taacatttct ttctttttct atgatagtta
     3781 aatcccagtg attttttata aattcaaggt ctttttcttg actctgattc atttaatcat
     3841 ttcctgttaa tagtataatt ttaaagctat tactttgttt tattttaaca tatttataca
     3901 ctaaagtgaa ttttatgatt agatttattt aattatttct gaattaaaac ttattagaat
     3961 tctctatttt gataatttca aactctattg attgtatgat aattgaaact ataggatatg
     4021 taggataaca agtttcaaag tagttaaaat aaaaaagata atgatattat taatttagaa
     4081 taaattttat atattatatt taattattta cttgttttaa attttattaa cttaaaaaat
     4141 gtaagaaatt tattatcaat aatctaaata tagaaattaa aaatgataaa gagattgtaa
     4201 ttatattttt ttgaaacttt gtgattttat ttgtcgaata aaaaataaaa aaatttaaag
     4261 gaaaaagtta aaaaataaag aattttataa ctataaatta tattttagag tttattgtta
     4321 ttaaatagat gaaaaaatcc taatgtggat tatcagaaaa tgaggaggtt gttaattgat
     4381 aaaatgtctt gaaatgtctg taaaagtgta tcttcttgaa aatatttctt tagataatac
     4441 atcaaaagaa atacgtaaat taatagatga tacattaatt atagaagaaa aatataagga
     4501 atttcataat aaagctggat ttaagaatta ttcttttaat ttattatatc cattagaaaa
     4561 agataaggtt tataagaagg attgtatata tacttttatt attagaactg tagatagtga
     4621 attaggaatg tattttatga ataatttaaa aaatcagtat tctgataaaa taaaggttat
     4681 tacaatgagt aaaaaagtga tccctaaaaa acatatagaa agtatttact ctgtcactcc
     4741 aataatagct aaatttgagg atgggtattg gagaagtaat aaaacagtag aagatttaga
     4801 aaaaagaatc agagaaaatt taataaaaaa atataatgaa tttactaaca gtaaaataga
     4861 tgaagatttt gaattatttc aagttattaa atttataaat aggaaaccta tttctactaa
     4921 atataagaat atatctttac ttggtgataa aataagtttt aaagtatcag aaaatgatat
     4981 ggctcaggaa attgcaaatt ttgcacttgg agttggagtc ggagaaatta attctcgtgg
     5041 atttggtttt gtaaactata gatggttata ggaggtgaaa acttgttaaa ggattgttta
     5101 gagatttttg aaaaagaata caaatcttta ggaaatagat tgataactga tagctatgtc
     5161 ctatcctatg gaacatatat attggtgaaa aatttttcga atatagatat attagaagtt
     5221 tctaaaaata atgtggatag tacagaagat atataccata aattttgtga actggattat
     5281 ttatcaaaac tggtgtctat agataaatca gttgattcca aaaaaataat acatagtaat
     5341 aattatcttt cattttttgt aaaaaaggaa aacttaagac ctgataaaaa taataatgta
     5401 aaacttacag aagaaattat tgataattat tatgatgctt tgttaaaccc aagaattaaa
     5461 tataagtctg ataaaagaaa aaaagaaatc tatgattcaa tagaaaataa atatggtaaa
     5521 gtggatgaaa ataaattaaa taaaataaga ctatggataa aaacaaatat atttgattta
     5581 gttgaatctg ttaaaaaaga taaaaattat ttaaaaatat tttttgatga aaaaatagaa
     5641 ttatacaatg atgaaaacca aaaatattta tatccaaata tatataataa aaatgatttc
     5701 aatgtaatta ttgatggtaa aaactatggg ttaccaaacg ataatatgca attaaatgaa
     5761 aaaaagccat ttttgaaatt aaaaacaagg attagaaatt caactcctta tttgattagt
     5821 gatgatgaag ttttgttaca gaaagctttt tttgattatc taatgaataa agcagcgaat
     5881 ggagaagaag tagtatatat aagtagcgaa aagggaattc atagaaaaac tgatatacca
     5941 aatgaattta gtggttatat actgagaata aaaaagggta aagaattaga aatacatgat
     6001 tttgatactg tggtaaattt aaacactaaa ataaaaagaa taaatgtaga atgtataata
     6061 ccaatagaat atgaaagaca taatttaaaa ccaaatttag agataggata tattaataat
     6121 cttataaaat tggaaaaaac gattgatact atattcttta ataaatttat gtgcaatagt
     6181 tattttaaag aaattaaaaa tataaactta agtgatttag ttctaaggga tatacttatt
     6241 agacatagag aaggtttata tacctggttt tataaaggga gagataatat agtaaagcaa
     6301 actttttcaa actattctat gggtataatt gaaaattcta taaaaaacaa taactacata
     6361 agagcaaagg agcaatataa tttaagaata gctataataa attattttca tggaggaagt
     6421 aatatggcag ataaaataaa agttattagt gaatctttat ttgaaaaaat aaattctata
     6481 aaaacgaaat gtatacaaag tgatgaagaa tattattttg cagttgggca attagcatca
     6541 tattttatat ctaaaaataa gtcaaaaaat aaaaaccatt ctctgattaa tcctatacta
     6601 aatgcacaaa atagaaaaaa aatcatgtta gagatagaaa aattatttaa aaaatataat
     6661 tatgatataa gttatgacaa caataggcga tttaagaatc tatattctat gattgtagga
     6721 tatgaggtag aagaaaaaaa agttaataga gatttattaa ttggtggata tctatattac
     6781 agtttaatat acacaaaaag tgaggagaat gaaaatgaat agaagagtat atggagtatt
     6841 aggtataaaa tctataatgt caaactggaa tgctgatttt tctggatatc caaagacgac
     6901 atcaacagga gaagtttttg ggagtgataa agcacttaag tttcccatga aaaaaatgtg
     6961 ggaaaatgaa ggtgaaaaag ttttatatgt taaatcatta aaaatagatg aatctaagaa
     7021 aggagaaata aaaattagac caaaatcatt aaaagagaga tatgaagaat tgtttgatat
     7081 agaggaacta aaactagaaa agaatcaaca aaaagtactg gaaaatctat ttaaggctat
     7141 tgatgttaaa aattttggtg caacatttgc agaagaagga aataatatag gaattatggg
     7201 cgcagtacaa attggacaag gatttaataa gtatgaagat attaatgttg aagaacaaca
     7261 aatattatcc ccatttaggg atccaagagc aaaagataag aaaaagtcaa caaatgaaga
     7321 agaaaaagaa gatgcaaatt caactacatt aggaaaaaaa atagtaagtg atgaggctca
     7381 ttacttttat ccattttcaa taaatccaaa agcttatgaa gtttttgtta atatgggtgt
     7441 gactgatgga tatactaaaa atgattataa aaaatttaaa gaagctgctt tagtaagtgc
     7501 aactgcattc aatacaaatt caaagcttgg ttgcgaaaat gaatttgcat tatttgttga
     7561 aactgatgaa gagttatacc taccagatct ggcacaatat ataacttttg aaaagagaga
     7621 aaaatcaaaa gatttaattt gtataaatat aattcatctt atagcagaat taaaagatga
     7681 tataaagagc gttgagatat attataaccc atataaaata gaaataaagc cttttaaaga
     7741 tgaaatatta cctaagatag ataaagttaa atttatgaac atatttacaa gaaaagaggt
     7801 ataatatgga ggttttgaaa tttaacttaa gtggaaaaac tgcttttttt aaaaaaccag
     7861 atgtaaatag cataatttat tttacgtatg gaaacataca taaaattgca cttatgggta
     7921 ttttaggagc ttgtttagga cttggtggat ataatcaaca gagtaaggag gatgagtatc
     7981 cagaattcta ctctttactt aaggatataa aaatgtcaat agttccaaag catgaatttg
     8041 gatatataaa taaaaaagtc catatattta ataattctgt aggatatgca tcaaaagaag
     8101 aaggtggaaa tttaataata aaagaacagt ggcttgaaaa tgttagttgg gatatatata
     8161 tacaattaga agattcaact acaagtagag agcttaaaag aagattttta tctaataaat
     8221 ttgtatatat accttactta ggcaaaaatg atcatatagc taatataagt gaaatagaga
     8281 taattactga tttttacaaa acagaagata ttaacagagt agacagtttc tttataagag
     8341 aggattttga gtttgaaaaa tcttattttg atgataatga tgattatgaa gagccattta
     8401 aatatcagga aaaacttcct tatagactgc accgaatgac taatcaatat attttggaaa
     8461 ctatcatatt tactaatatg tcagttaaat tgattaataa aaaagaggta tatcaagtaa
     8521 agggtttaaa tttaatcttt atttaaattt ataggtttag gagaattata tgtactttaa
     8581 aaaagttgag ttgtttgata taagtaataa tataaataat tgcaaaaata tttatgctca
     8641 tataaataaa gacaggaagc cagaaacttt aaaagaacat tcagacttat catactcata
     8701 tcttatgaaa ttatgtgtaa aaaaagattt aagctcttgc ctagagagaa tagagaataa
     8761 attttttaaa aaagattctt ttttaagtaa gaatttttat agagagatta tcttgaatat
     8821 ggtttatatg catgatatag gtaagattaa ttcttcattt cagtttgtaa aaatgcaaaa
     8881 taaaaatttt aaaaaaattt cacttagata tactaaacat tcttttatct cttcaatgct
     8941 atattttgat tactattttg aaaagcttaa aaaatatgat tttgaagaaa aaaaaatatt
     9001 gagtttattt ttgatgataa atacatatac aatatcaaaa catcatgggg ttctgaagtc
     9061 atttaaagaa tttttatttt tatttaaaga tgagtttata aacattaaag aaaattttaa
     9121 agatttattt aaaaacaatt ataatttaga gcttaaatca gttcccaaaa ctatagaaaa
     9181 aatggtaaga ggtgtagaag ggtatttaga tgatagccat ataaaggata acttagctat
     9241 agacttatat atatattcaa aattgatgtt ttcaattgtt acatcttgtg atttttatgc
     9301 tacatcagaa tataatagtg gtaattctat agatggtttt gggacaattg ataattataa
     9361 taagtattat gatgtattaa aaaaatctga aatatataag gggatgaaaa aatataggga
     9421 atacttaaat aacaatagtg aatgcccata taaagagaat gatataaaca gattgagaac
     9481 agaaatgagt atagaagctg aagatgcaat tacacataat acgagaaaaa gaatatttta
     9541 tttagaagca ccaactggaa gtggaaaaac tgttacatct ataaatctag cactgaaatc
     9601 tatagagtta aatcaatctt taaaaaagat attttatata tttccattta acacattagt
     9661 agagcaaaca aaagatgtat ttataaatga aatatttaat tctgttaaag acatacaaaa
     9721 agatgttgta gttataaatt caataacacc aatacaaaca gaagataaag aagaagataa
     9781 taaaaatgtt gaatattgtg ttactaaaca agaaattgac tatgataaat cattgttaaa
     9841 tagacaattt ttacactatc ctatagtact tacaactaat ataggttttt ttaactattt
     9901 gtttggtgta tcaagagaag aatgttttcc attgatacat atgataaata gtgtaattat
     9961 attagatgaa atacaaaatt ataaaaatga gatatggaaa gaaattattt tgtttttaga
    10021 aaaatatgca gatatattaa atataaaatt tattataatg tctgcaactt tacctagatt
    10081 aaataaatta ggatgtaatg atgataattt tgcatatcta gtaaaaaaca gagaaaaatt
    10141 ttttaaaaat caccttttta aagatagagt aaacataagt tttgagatgt taaattcaga
    10201 aattaatgat attgaagaaa tatcagaaaa ggttatagaa gaagcagaaa tatataataa
    10261 gatactgata gagtttataa agaaatcatc agcactagaa ttttataaaa atattaaaga
    10321 aaaattgaag tataagttag aagatgtttt tttattaaca ggtgatgata ataaattaga
    10381 aagaaaaaaa ataataaata aaattaaaga tatgaactct gtaattttgg ttgctacaca
    10441 agttgtagag gctggagtag atatagatat ggatttggga tttaaagata tatctacaat
    10501 tgattctgat gaacaatttt tgggaagaat aaatagatct tgtaaaaaat taaattcaaa
    10561 agtatacttt tttaatttag atgatgcaag taagatatat aagaaagatg ttagaaaaga
    10621 gagacattta actcttaatg aggaaagtaa tagaaaaatt attttagata aagactttga
    10681 aaagtattat gataaaataa tagccagaat agaagaaaat aagcataaac ataatgacaa
    10741 gaatatagag gtttttataa aagatattgt gggaaatttg aattttacag aggtaaaaaa
    10801 atggatggct ttaataccag atttaaaaga gtatacaatc tttttaaata ctatagttaa
    10861 agatgaatct ggaaatatga ttgtaggaag tgatgtatgg tatgaatata tatcactact
    10921 aaaaaatgat aatgttgagt attctgaaaa gagggtgaaa atgtctgaaa ttatggaaaa
    10981 actagattat tttacgtata aagttcagaa atttgataat tcatttaatg atttagttgg
    11041 agatattttt tatattgatg atggaagtaa gtattttaca gagggaaagt ttgatagaag
    11101 taaatttaat caaaatgaat ttctataatg tatttagatt atatagagaa tatttatccc
    11161 aagttaatta attagaatta gtttaaagct attgaaatat agatatttta tatcttatat
    11221 atattttcta ggattagctg ggataaaaaa tctgtatatt agtgttttca gtgtatttaa
    11281 gggaattata tttttaagaa gtactgattt taaagtggtt aaacagtaac atgagatgta
    11341 tttaaatatg tttaagtcag atggctcacc aaatcaaatg tttgttaaac agtaacatga
    11401 gatgtattta aattttattg tagctgttat aacttcttta tttgtattag ttaaacagta
    11461 acatgagatg tatttaaatt gatttattag aggaaaatag agaattaaat attaagttaa
    11521 acagtaacat gagatgtatt taaattattg ctctaaattt agtagaccaa gttcaatttc
    11581 tgttaaacag taacatgaga tgtatttaaa tttaaatgct ttataaagta ttaattcatt
    11641 ttgtaacgtt aaacagtaac atgagatgta tttaaattat taatccttct attggaaaat
    11701 atcgtcttca agagttaaac agtaacatga gatgtattta aattatagtt ttgtagctct
    11761 ttcaagttca tctgcatttg ttaaacagta acatgagatg tatttaaata tatatctaaa
    11821 tggattatta tatctaactc catctgttaa acagtaacat gagatgtatt taaatatttt
    11881 ataatggtaa aaaagagttt tacatagctg gttaaacagt aacatgagat gtatttaaat
    11941 ttttgaagtt tagttatatc tgggtcacca gtctgtgtta aacagtaaca tgagatgtat
    12001 ttaaatcagc atctaaacct tctcttcttg cttcctctga agttaaacag taacatgaga
    12061 tgtatttaaa tttaaaattc aatttagtaa atgaagttaa ttctggagtt aaacagtaac
    12121 atgagatgta tttaaatttt tcttttgcag cacttcctag aacatctcca tcagttaaac
    12181 agtaacatga gatgtattta aattcaaatc taactgatgt tcttttatta tcaaaaggtt
    12241 aaacagtaac atgagatgta tttaaattct ttagcttttt ctatttcagc aattatctct
    12301 actgttaaac agtaacatga gatgtattta aatggattat ccaatcttta atcttattac
    12361 cataccattg ttaaacagta acatgagatg tatttaaatt atctcttaaa gcactatcta
    12421 attcaacaat agttggttaa acagtaacat gagatgtatt taaattattg aacttagcat
    12481 ctaatttagt atttttagta ggttaaacag taacatgaga tgtatttaaa ttagaataaa
    12541 aattacttgc agaataaaaa atgtcttcgt taaacagtaa catgagatgt atttaaatta
    12601 gaaaaaagtg tacattgaca actgaatatc ccctgttaaa cagtaacatg agatgtattt
    12661 aaatacactt attttgattt tatttatttt aattttttat gttaaacagt aacatgagat
    12721 gtatttaaat tccatctact tttttacttt tagctttttc tttagtgtta aacagtaaca
    12781 tgagatgtat ttaaatagtc tgaagatgag cgaatgtgct atatctgtaa tagttaaaca
    12841 gtaacatgag atgtatttaa atttaaatat aataaatcgt aatgtacaaa aataacttag
    12901 ttaaacagta acatgagatg tatttaaatt ttcttgagtt aattgaaccc ccatttttaa
    12961 gctccgttaa acagtaacat gagatgtatt taaatttttc ttctagctct tttttcttag
    13021 ccattttacc agttaaacag taacatgaga tgtatttaaa tatgtttata catgagttta
    13081 gtgataggtt cttggtcgtt aaacagtaac atgagatgta tttaaataag gcaagtgatt
    13141 tgctatttag aataggtgta aacgttaaac agtaacatga gatgtattta aatatttctt
    13201 tttgtgaagt gttaagagat tgctcttcgt taaacagtaa catgagatgt atttaaatac
    13261 agtagctatt ccaatttctt ctgcttgtgg catagttaaa cagtaacatg agatgtattt
    13321 aaattcaaag atgcccctct agctggtttt tgggtatcta gttaaacagt aacatgagat
    13381 gtatttaaat tgtgttgagt cgtgatattg actttttcgg aaacgggtta aacagtaaca
    13441 tgagatgtat ttaaatttgt agtccttcca tttccatcac acaaaaagca tgttaaacag
    13501 taacatgaga tgtatttaaa ttctatgtta tgtacatgat taccttgttt gagtaggtta
    13561 aacagtaaca tgagatgtat ttaaattaag agtctctttg tatatcaatt tctctagtag
    13621 atgttaaaca gtaacatgag atgtatttaa attaaattat tagttttaaa atttaaaggg
    13681 ggaaaaaagt taaacagtaa catgagatgt atttaaatgc taacttaatt acttgttcca
    13741 catttgctaa taccgttaaa cagtaacatg agatgtattt aaataaatat tcaatatagt
    13801 ttttaaatac actttccatt gttaaacagt aacatgagat gtatttaaat tcattagtac
    13861 ttgttattcc agtaaaagct actttggtta aacagtaaca taagatgtat ctaaattact
    13921 ctatcataaa attttaatat attaaacaaa ctaatatgtg atatacttat gtataaataa
    13981 caaaataaat aggtgatgtt atgaaaatta caggaacttt gataaattac tatttccatt
    14041 gtaaaagaca atgctggttg cttggtaata gaataaattt ggaagaaaat agtgaagatg
    14101 taaaaatagg tagacttctt catgaactaa aagaagaaaa atcaaaatat aaagaaattg
    14161 caattgaaaa tataaaaata gacaaattaa caagagaata tttagtagag gttaaaaagt
    14221 ctgatgctga tatagaagct gttaagtggc aagttctttt atatttaaaa aagttaaaag
    14281 aaaagggaat tgtaagaaaa gggaaaattg aatttataga aaaaaataaa actaataaaa
    14341 aaatagtctt tgtagatttg agtgaagtat ctgaagaaca gcttatatct atagaaagag
    14401 aaattgttaa tttaataaat agtgataaag caccatgtcc tgatttaaaa aaaggatgta
    14461 aaaaatgtgc ctattatgaa tattgttata tataaagagg tataagaaac atataaggag
    14521 gtgttaatat tgggaaagac aaaatatatt acctcaatgg gtgaactcac aagaaaagat
    14581 aattctatat gttttagaaa aaatggaagg aatgtatata taccagtaga aaatactagt
    14641 gaaatattct gtctaaatga ggtatcttta aattcaaaat tactggattt tttatcaaga
    14701 gcaaatattg ttgttcattt ttttaattac tatggtggat atagtggaac tttttatcct
    14761 aaagaatatt taataagtgg aaaagtaaca gtagcacagg taaaaacctt tgaagataga
    14821 agagaagaaa tagcaaaatc catagtcaat ggtataagga ttaatgcaat aaatacatta
    14881 tcgcattact ataagcatgg aaattcagaa atacaaccat tattatcaca tctaaaaaaa
    14941 gattctataa acaagttaga aaaatcaaat aatataaaag aaatattatc agttgaagga
    15001 gatatttggc aaacatttta tacttccttt aagtatattt taagagagga atttattttt
    15061 aataaaagag ttaagagacc accagataat cccttaaacg caatgatatc atttggtaac
    15121 agtattttat attctaaaac tgtaactgct atatataata cacatctaaa tcaaagtgta
    15181 agttttttac atgaaccaag tgaaagtaga ttttctttaa gtctagattt aagtgaaaat
    15241 tttaaacatg ttataacttt taaaactata tttgatttag ttaataatag aaaaataaat
    15301 attgaaaaac attttgataa aaatttgaac tactgtgttc taaatgatga aggaaagcaa
    15361 atttttataa attctttaga agaaagatta gaatcagtca aagaacatcc aaaattaaaa
    15421 cgaaaagtaa gtttaaaaac ctgtataaaa ttagatgctt acaaaatgtt aaagtgtata
    15481 atggaagaaa aaagttttat tccatataac ataaaagacg gattataatt taaacataaa
    15541 gtaagtattt taatatagaa ctttgatata tataagaatg ataattatgc tattataagt
    15601 atacataata tattattgaa ataatataag tttttagaag tatttcaaga tgagaaagga
    15661 gaaaatactg aaaaataatt attctattat aatttgccaa ggttttgtct atttttcagt
    15721 ttaaatagta tattacatat gaaaaagaaa aacaatttaa attataatta tgtatttgta
    15781 gtctatgatg taggagaaaa aagagtaagt aaagttttta aagtgtgtaa aaaatatctc
    15841 tcgcattttc aaaaatcagt ttttagaggt gaaataactc catctaagat tatgagctta
    15901 aaacgtgatt tagaaaatat tatagataaa gataatgatt ttgtatgtat aataaagttg
    15961 atgaattcta atacatttga agaagagata ttagggaata atagtaattg tgaaaatatg
    16021 atattataaa tctaattact atatttgtaa aaaataatag cttattaaag agtagtaaaa
    16081 ttgggatata atttatgata gagactgcaa gtagattttt gtaaaaagga tttacttgca
    16141 gtatttttgt tatgtataac tgttaacatt agtgtacaaa acattatcaa tagcattttt
    16201 ctataatgaa gaaatactat tataatggga aaattttatt aatactaggt aataata
//
LOCUS       DAMJVM010000075        16112 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.078, whole genome shotgun sequence.
ACCESSION   DAMJVM010000075 DAMJVM010000000
VERSION     DAMJVM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16112
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.078"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            41..487
                     /locus_tag="PC523_002609"
     CDS             41..487
                     /locus_tag="PC523_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2476117.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNRIKKEFSDEEYKELVKKLHVVKDIKTAMEEE"
     gene            489..1583
                     /locus_tag="PC523_002610"
     CDS             489..1583
                     /locus_tag="PC523_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HDF2476118.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFCDIAKS
                     GVALITLGDVSVRPSFHNMAMSLTDEDGVMKYKKIVDEIHNSGAKVSAQIFCSDYDVN
                     LIKDTMKMGITSHVEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLIGSFSSSIFNNRNDEYGGTCENRSRFASEIISSIRNEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLFSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPINNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMISMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTHSQFGCILD"
     gene            1665..2195
                     /locus_tag="PC523_002611"
     CDS             1665..2195
                     /locus_tag="PC523_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2476119.1"
                     /translation="MKSLILYSSLTGNTKKIACSIYEAIQGKKEIKNISELVDDSIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASTKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKVKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EANVFAKNMMIKASTM"
     gene            2522..3355
                     /locus_tag="PC523_002612"
     CDS             2522..3355
                     /locus_tag="PC523_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2476120.1"
                     /translation="MTIKEVEEKTGLTRSNIRFYENENLIKPLRNERNGYREYSDENI
                     EDIKKIAYLRTLGITIDDIRNIILQKVSLVEVVEKQEKVLETKISDLENAKTMCNKML
                     ESDELSYLNLKVEQYITELPEYWNKNKPVFKLDSVSFLYIWGRKITWGIITIACLLIA
                     IIVFPKLPHQIPIQWNNGVANYVDKKFIYAYPAICVVFRILLRPFIWRWLQIHAFYSD
                     TIADYLTNFMCFVALSIEIFSILFIYNIARHVTIIIFIDIFVFIALLIIGVYKLFKKY
                     N"
     gene            complement(3605..4813)
                     /locus_tag="PC523_002613"
                     /pseudo
     CDS             complement(3605..4813)
                     /locus_tag="PC523_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5002..6114)
                     /locus_tag="PC523_002614"
     CDS             complement(5002..6114)
                     /locus_tag="PC523_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDF2476121.1"
                     /translation="MKISEVSTPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKLECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     AEAVSKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIERVIEVDIRENSKQIIL"
     gene            6412..6993
                     /locus_tag="PC523_002615"
     CDS             6412..6993
                     /locus_tag="PC523_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDF2476122.1"
                     /translation="MLEVVNMRGVVNMRKVILYIATSLDGYIAEEDGSVSFLDEISSV
                     FKNEQEDDYNTFYDTIDTIIMGNSTYVQIANELSPNEWYYKGKECYVYSNIINEITEN
                     VKYTNLEPKKLIEQIGREKDKKDIWIVGGGDIVKLFMKDNLIDDYYIYILPIILGRGI
                     SLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            7238..8965
                     /locus_tag="PC523_002616"
     CDS             7238..8965
                     /locus_tag="PC523_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2476123.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSVLLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKENENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            8965..10755
                     /locus_tag="PC523_002617"
     CDS             8965..10755
                     /locus_tag="PC523_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2476124.1"
                     /translation="MKKSKSFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGIPGASVNFDYVTKILVILACIYVANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQNRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADTESE"
     gene            complement(10916..11224)
                     /locus_tag="PC523_002618"
     CDS             complement(10916..11224)
                     /locus_tag="PC523_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476125.1"
                     /translation="MFNKVYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(11486..12034)
                     /locus_tag="PC523_002619"
     CDS             complement(11486..12034)
                     /locus_tag="PC523_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476126.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILKKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            complement(12136..12807)
                     /locus_tag="PC523_002620"
     CDS             complement(12136..12807)
                     /locus_tag="PC523_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2476127.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEIIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIIEIFQIRSAIEGFCTFLITSQYKEA
                     RAIETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYKTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            13191..14096
                     /gene="garR"
                     /locus_tag="PC523_002621"
     CDS             13191..14096
                     /gene="garR"
                     /locus_tag="PC523_002621"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDF2476128.1"
                     /translation="MEVYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDIDKSAVDEL
                     ISCGAKYASVLEMGRECDIVFTILPNGTIVQDVLFGADGLAKTLKEGSIVVDMSSVTP
                     TESVLCANKLKERGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SLLIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHNN"
     gene            14232..15614
                     /locus_tag="PC523_002622"
     CDS             14232..15614
                     /locus_tag="PC523_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2476129.1"
                     /translation="MITGYGLLVAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFRISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            15686..>16112
                     /locus_tag="PC523_002623"
     CDS             15686..>16112
                     /locus_tag="PC523_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDF2476130.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPVETNLLKSEVERLSE"
BASE COUNT         6021 a         1914 c         2580 g         5597 t
ORIGIN      
        1 tatagtcaaa tatattataa ttatagaggg gaaatttaag atggatttta aaaatttgca
       61 gtatgaaagt ttgtcatata ttatttgtaa tttacaaaaa aactttaaac tttattgtga
      121 aaaatgttta aaaccatata aacttactaa tggtctatat ttttatttaa tctatattaa
      181 taaaaataga aattgttctt tgaatgatgt ttctacagaa tttgaagttg ataaggctca
      241 tacaacaaga actatatcta gattagaaca agatggatac atagaaaaaa tccaaaaccc
      301 taatgatagc agagcttttc agttgagagt aacagataag ggagaggaag tattaggaga
      361 tataaaaaat atattttcaa aatgggataa ccggataaaa aaagagttct cagatgaaga
      421 atataaagaa ttagtgaaga aattacatgt ggtaaaggat ataaaaacag ctatggagga
      481 agaataggat gtattgtaat ttgttaaagc caattaaaat tggaaatttg gaattaaaaa
      541 atagagtatc atttgcacct acaagcatgg gacttaaatt agaagaaaaa attaaaaaat
      601 tttgtgatat tgctaagtca ggagtagctt taattacttt aggagatgta tctgttagac
      661 ctagctttca taatatggct atgagcttga cagatgaaga tggagttatg aaatacaaaa
      721 aaatcgtaga cgaaatacac aattctggtg caaaggtatc agcacaaata ttttgttcag
      781 attatgatgt taatttaatt aaagatacta tgaagatggg aattacttca catgttgaga
      841 taaaaaaaat aatgaatgat ggagtaaaag attacataac aaatatgccc aaagaagaaa
      901 ttaaaaatat tataaattta tttaaagtaa cagcattaaa tgcaaaaaaa gcaggatttg
      961 acatgattca aattcatgga gataggctta taggaagttt ttcatcaagt atatttaata
     1021 atagaaatga tgaatatggt ggaacatgtg agaatagaag cagatttgca agtgaaataa
     1081 ttagtagtat ccgaaatgaa gttaaggata ttccaataga ctataaattt gcaataagac
     1141 aagaaaatcc tcattatggg aatgcaggag ttttattttc ggaagttgag tattttgtta
     1201 aaaaatttga atccctaggt gtaaacagtt ttcatgtaac acttgcaaat cattcaaaat
     1261 tggaagatac tataccaatc aataaccatc cttactttaa agatgaaggt tgttttttat
     1321 atttagctga tgaagtaaag aaacacacaa atcttccagt atgtggggtt ggaaaattgt
     1381 ctagcccaga ttttatagaa agtattattt caaataatag agtagatatg atttctatgt
     1441 caagacaatt actagcagat agtaattggt tacagaaagt aaaagatgga agagtagatg
     1501 agattaaaaa atgctgttat tgtaataaaa agtgtgcaga tgccttgcag acacattctc
     1561 aatttggatg tatcttagat taaattgtgt ttgtagaaat aaaaaatctg ttaatatttt
     1621 tcttgtaagt tagataatta tttagataaa taggaggata catcatgaaa agtttaatct
     1681 tatattcaag tttaactgga aatactaaaa aaattgcctg ttctatatat gaggcaattc
     1741 aagggaaaaa agaaattaaa aatataagtg aattagttga tgatagtatt gactatgaaa
     1801 attatgatat agtattctta ggctattggg tagataaggg aatttgtgat aaaaattcaa
     1861 aacaagtact tgaaaatatt cacaataaaa agatagcact atttggaaca atgggagcaa
     1921 gtacaaaagg aagttatgga gctagtataa tagaaaagat agaatcaatt attccaaaag
     1981 ataatgaaat attaggttca ttcatatgtc aaggtaagat agcagaagga ttgaaagtaa
     2041 aatataaaga aatgttaaaa ctttctcctg ataacgaaca tattcgccaa caattaaata
     2101 accatgagga atctcaaagt catccagatg aacaagaaat atatgaagct aatgtatttg
     2161 caaaaaatat gatgattaaa gctagtacta tgtaacagtt aaataaagag tttatatagg
     2221 atatagaatc aataatccaa taaaaagtat agcacatgat ttgctatgct ttttttatga
     2281 aattaaaaaa ataataaaaa tacttgactg taaaccttgt ttatacatta ttttatagaa
     2341 aataagatag ttttaaatat agaaaaagag atattattga aaggagatat tattgtatga
     2401 aaatttataa aaggtaatta gtttgatttt aagacataat aaacatacaa ttttatagag
     2461 tgaaagtatt taagactata ttatagctat aaattaatag aatagagagg tagtgtttaa
     2521 aatgacaatt aaagaagttg aagaaaaaac aggattgaca cgctctaata ttcgttttta
     2581 tgagaacgaa aatttaatta aaccccttcg taatgagcgt aatggctata gagaatattc
     2641 agatgaaaat atagaggaca tcaaaaaaat tgcatattta cgtacattag gaattactat
     2701 tgatgatatc cgtaatatta tattacagaa agtatcttta gttgaagttg tagagaaaca
     2761 ggagaaagtt ctcgaaacta aaatatcaga tttagaaaat gcaaaaacta tgtgtaataa
     2821 aatgttagag tctgatgagc taagttattt gaatttgaaa gtagagcaat acataactga
     2881 gttgccagaa tactggaata aaaacaaacc tgtttttaaa cttgactctg taagtttctt
     2941 atatatatgg ggaaggaaaa ttacctgggg aattattact attgcttgtt tgttgattgc
     3001 aataatagta tttccaaagt tgccacatca aattcctatc caatggaata atggtgtagc
     3061 taattatgtg gataaaaaat ttatttatgc ctatcctgct atttgtgttg tattcagaat
     3121 ccttttacgt ccatttatat ggagatggtt acaaatacat gctttttata gtgatactat
     3181 tgcagattat ttaacaaatt ttatgtgttt tgtagcgttg tctatagaaa tattctctat
     3241 actttttatt tacaatattg ccagacatgt tacaataatt atttttatag atatatttgt
     3301 atttattgct ttactgataa taggcgtgta taaattattt aaaaaatata attaactttt
     3361 aaaaataagt aataagtgat agtgtaaaaa gtagccactg caaattatgg attgtagtgg
     3421 ccattttatt atttgtatat gaaattattt tatttttcta ataagctacg atatattatg
     3481 gtttatttca ctgataaaag atttaggtat tttaaagtaa aaagttgtat attctaataa
     3541 aatttaaaca ttttgattct ttaaaattag agaaaataca ttgcttttat taaaaattta
     3601 aatattaaga atttaatttc tgcatcttat ttagaaaaga taagaaatca attttatata
     3661 ttttttttgg tcttcctctt tgattatcga gttttgtatt tacaatagtt gcaattcctg
     3721 ctttatataa gatactcaat attctattag cacttctaag agttatgttc atatagtctg
     3781 ctaatatttc tgctgacaca ttatttgaat ttctatcatt aatcatacac atgattttag
     3841 acacatttgt attagataga ggtataaaag aagcaacttt attggctatt gagctatctt
     3901 caatagtatt agaattttta tttgaataca aaggtcctat tgtatcatta gtatcattta
     3961 ctataaaagt acagtttcca tttagagcag cagatttacc attagcttga ctagcatttt
     4021 gttctgcatg aatatttgta tttccaatac cccaacctat atttaagcta tatttaattt
     4081 cttttaaatc ttctgaaata aaaaaatctg tatagttatt ggtcatattg tttaacattt
     4141 ccttagtagt atgtatctct atcttcatat catttttttt gacctggact ccataaaatc
     4201 catgtcttga tagagaattt aagatagtgt tatatatttg gttttgtatc tcatcatttt
     4261 taaatatcaa atcttcattt atgtactctg aactatttac tgtaactata caagttgcgg
     4321 atttattttt gtctaagttt tgtaatgtta tttcattcac cagagattta aatgtattca
     4381 agattgattc tgtagatgga accatgtaaa tacaatcata tccaagctta ttaagttcac
     4441 aactaatgat gtaatttcta gtaaaagaaa ggttaatctt attatctcgc aataagtcta
     4501 aatgtatttc caataattta tcatttaatt ttttaaagtc atacacaggt tgagggatgt
     4561 taaaatcttt gattgtatat ggacatttat tagaatctat taatgaatat atatcataat
     4621 aattgttgtc ttcttttaaa aaatccataa atgattttgt aaagtcaata tctttttgac
     4681 tatttaatag tttgaataag tatttataaa aatctctttc atcaagcttt aaaaagttta
     4741 atggtacttt tatgtctttt atatttatta taagttcact atatggaact ataccactac
     4801 atacaaatcc atttatatga aataaagtat attttcataa tttcaacaat gaaagagagg
     4861 cttatctcta ttttgagata gcctcttctt ttaaatttat atttctagaa tttatgtttt
     4921 tatttataca acattgacat tagttattaa caataatttt gagaaaaata tactttcgct
     4981 aaaaccaaaa taaatgtatt tttaaagtat gatttgcttt gaattttcac gaatatctac
     5041 ttctatgact ctttctattg tatctcctct aaaacctaat aaattactcg tcaagtttgc
     5101 agtggaacaa gaatgattag gtataattct tattctatca ccaattttta tatcagtttc
     5161 accatttact ttaatttttc caacttcttc tgaaagtgaa tcaatagtaa gttctggatg
     5221 tccacatacc ctaccataac caacaatact actgtttcca tgtgcacctt tatcaagtcc
     5281 aaggcactta cttccacaat caattaaata gtatccactg ctattttttg agattacagt
     5341 gcacacaaca gtaagagcac acatatcttc agtagcacaa cctaaaccta tctgtatagc
     5401 atcatagtat acatagtttc caggtcttac agcagttata gttttagaaa cagcttcagc
     5461 ttcaactgtt ggagtactac cagaagcgac aatttctata ttaaatccat tatcagttag
     5521 agctttgtat gctttctcca tagaagaatc ttcttggaca caaacatctg gaacaccttc
     5581 tggagagcta caaccataca cttgacctgg atgtgagctt ataccaacaa tcttgatgtt
     5641 ttctagctta gaaatttcag tagcaagttc aaccactttt tctggttcaa ccccaaaacg
     5701 gtgtaagcca caatctacaa taataagtag tttacactct agttttgatt tagaaaaagc
     5761 ttcactataa gccttagcac attctatatt atctgtagca caaattaaat ttatttttga
     5821 tgccatagta acaattctat ttatatttac ctctcccatg taaggataag ccaacataat
     5881 atcttcaaaa cctgctttta tcaaagcttc accttcatca agtgttccaa ctagaaaacc
     5941 actagcacct gcattttttt gaagtctcgc tatgtaagta cttttatgtg ttttaagcat
     6001 aggccaaagt tgtttattat tatttgtaca aatttgttga attttatcta tattcttttc
     6061 taactggtct aaatctagca aaaaatttgg agtagaaact tctgaaattt tcatgttatt
     6121 taccaccttt caacaaaagt aaattaacta taagtattat aattattata ttagtaatat
     6181 cggctttata atattaatag cttataatta tattataaaa ctagcatgaa cagagagcaa
     6241 tgtttatttg taataattag aattttcaat aaattattgg agttttaaat tatttatata
     6301 acatattttt aattttatgc aaagtttatt taaattagtt tgaggattta ttaatagggt
     6361 atcattgaat gagtaaaaaa gtgatgtttg catagaataa ttacactata aatgttggag
     6421 gtagtaaaca tgcgaggggt agtaaacatg cgaaaagtaa tactttatat tgccacaagt
     6481 ttagatggtt atatagctga agaagatggc agtgtaagtt ttttggacga gatatcttca
     6541 gtctttaaga atgaacagga agatgattat aatacttttt acgatacaat tgatactatc
     6601 attatgggaa attctacata tgtacagatt gcaaatgaat taagtccaaa tgaatggtac
     6661 tataagggga aggagtgcta tgtatactca aatataatta atgagataac tgaaaatgta
     6721 aagtatacga acttagaacc taaaaaatta attgaacaaa ttggaagaga gaaagataaa
     6781 aaagatattt ggatagttgg gggaggcgat atagtaaaat tatttatgaa agataactta
     6841 attgatgatt attatatata tatattgcca ataatattgg ggagaggaat ttcactattt
     6901 aagtctggta tagacaagac taacttgaat tttaagaaag ctagtaatat tggagaactt
     6961 gtaaaattgg aatacagtaa aaaacaaaaa taaattacat gccaatttgt tacaatgtta
     7021 tgaaatgaag catttaaaag acttttggct atatgaattg acattacttg tcaaatatgt
     7081 taacatataa gcgacttgtc ggagggtgta ttaaaaagaa tatgctggag aagagagtgc
     7141 gttaactctt ttctcctttt tttataaaat taagcattta taaatttagt attattatat
     7201 gattacacta gctcaaaaat taagaaagga gaaaaatatg aaattaattt taagttattt
     7261 aaagaattat aagttattaa tagtattaaa catactggct atatttagtt ttgcattagt
     7321 cgaattgggg attccaacca taatagctaa aataattgac aatggtattg caaatcagaa
     7381 tatagcttac ataaaacaaa tggggatagt aatagttatt atttctatta taggggttgt
     7441 aggtagtgta ttgttgggtt attgttctgc taaggtatca acaagtgtaa caagagatat
     7501 aagaaatgat atatttgaga aatcacaaga attttcacat actgaatata ataaatttgg
     7561 aatttcttca atgattacaa gaacaacaaa tgacgcattt caaattcaac aatttgttaa
     7621 tatactacta aggactgcct tattaactcc agttatgttc atcataagta taataatgac
     7681 aattagaaca agtgtggaac tttctatagt attggctata tcagtacctt ttataattat
     7741 tggagtagca ataatagcaa aagtatctca ggcaatatct tcaaagcaac aaaaaggact
     7801 agataaatta aaccttatat cgagagaaaa tttaactgga ataagagtaa ttagagcatt
     7861 tggaaatgat gattatgaaa ctgagcgatt tgaaaaaaca aatacatatt atgctaatgt
     7921 ttctaagaaa ttatttaagc ttatgtcaat tactcaacca gcattctttt tactattaaa
     7981 catagctgtt ttagctgtat tttggatttc aagtgagaag ataaacattg gagaacttca
     8041 agttggacag ttggttgcat ttttggaata tcttttccat gccatgtttt caattatgct
     8101 tttttcaatg gtatttatta tgtatccaag agcagaggta tctgcaaata gaataaaaga
     8161 acttttaaat gaagaaccat taataaaaaa tcctgaaaat ggaattaagg agaatgaaaa
     8221 taagggtatc atagaatttg ataatgtaac ttttacctat ccagatggag aagcttctgt
     8281 acttaaggat atttcattta ctgcaaaaac aggtgaaaca gttgctttta taggaagtac
     8341 tggtagtggg aaaagtactc taattaactt gatacctaga ttttacgatg taacagaagg
     8401 tagcattaag ataaatggag tagatataag agaatatgat ttaaaagctt taagaaaaaa
     8461 gataggattc atacctcaaa aatcactgct ttttactggt agcatagcaa ataatattag
     8521 atttgggaaa cataaggcag gagagtcaga attagaatat tccgcaaaag ttgcacaagc
     8581 atatgagttt atatcaaaaa aacctagaaa gtttgatgaa ttgattagtg aaggtggagc
     8641 aaatgtatca ggaggtcaaa aacaaagact ttctattgca agggctatta taagaagacc
     8701 tgaaatatat atttttgatg atagtttttc tgcacttgac tttaaaactg atgcaatact
     8761 tcgtgctaaa ctgaagaagg aaacaaaaga tgcaattgta ttgattgtcg ctcagagaat
     8821 tagttctatt attgatgctg ataagattat agtactcaat gaaggacaag ttgtcggaat
     8881 gggaactcat aaagaattat taaaaaactg tgaaatatac tatgaaattg caacatctca
     8941 gttaaaaaag gaggaattag aataatgaaa aagtctaaaa gttttaagaa aacaattggt
     9001 agacttcttc catttgtaaa gaaatataaa ttttccttta ttatagctat tatatgtata
     9061 atttcagcag ctactatgaa tgcacttgca cctaaaactg agggattgat aataacacaa
     9121 ttgactaaag atgtaattag tatagcaaaa ggaataccag gagcatccgt aaattttgac
     9181 tatgtaacaa aaatattggt gattttagca tgtatatatg ttgccaatgc aatttttacg
     9241 tatacttcaa gttttctact tacgaatgct attcaaaaca caatgagaga tttaagaaat
     9301 gaagtagaaa ataaaataag aaggttgcct attagttatt ttgatagtaa tagttttggg
     9361 gatgtattaa gtcgtatatc aaatgatgta gatacaatat caaatgcttt gcaacagagt
     9421 tttatgcaaa ttgtaaattc aattttagtc ataattttgg cactttcaat gatgtttaca
     9481 attaatatat atatggctct tatagctctt tttataattc caattagtta ttttgtatct
     9541 aaatttgtag taaaaaaatc tcaaaataga ttttcattac aacaaaatgc tcttggaaaa
     9601 ttaaatggaa aagtacaaga aatgtatact ggatttaatg aaataaaact atatggtaaa
     9661 gaagaagact ctataaaaga gtttaaaaag gtaaatcaag aactttgtga aaatggattt
     9721 aaggcacaat ttatatcaag tatgatgaat cctatggttt cattagtaac ttactttgga
     9781 attgcagcag tggcagttgt aggttctata tatgcagtaa gtggagggat aacagtagga
     9841 aatttacaag catttgtgcg ttatatatgg cagataaatc aaccactttc acaaatgact
     9901 caattatcca cagtaataca atcttctttt gcagctatag agcgtgtatt tgaaatttta
     9961 gacgaagaag aggaaatacc tgatgttgaa aatccagtta agatagaaaa tgtaaaagga
    10021 aatgttacat ttgagcatgt taattttgga tatggagaaa attcaacttt aatagaagat
    10081 ttaaatgcag aagtaaaaag tggtcagatg gtagctattg ttggacctac tggagcaggt
    10141 aaaacaacat taataaactt attgatgagg ttttatgatg taaagaaagg tgctattaaa
    10201 attgatggag tagacataag agatatgaaa cgtaaagatt tacgctcaat gtttggtatg
    10261 gtacttcaag atacatggtt atttaatgga accatttttg aaaatataga atacggtaga
    10321 tttggagcta ctaaagaaga gattatacaa gcagcaaaag tagcaaatgt tcatcacttt
    10381 ataaccactc ttccagatgg atacaatatg tttttaaatg aagaagcatc taatatatct
    10441 ttaggagaaa aacagctttt aaccatagct agagctttta tatcagaccc ttcaatattg
    10501 atacttgatg aagctactag ttctgttgat actaggttgg aacttatgct tcaaaaagca
    10561 atgagaaatc ttatgaatgg tagaactagt tttgttatag cacatagact ttctacaatt
    10621 cgtaatgctg acttgatttt agttatgaat aatggaagca ttatagaaca gggcaatcat
    10681 gatgaattga tggaaaaagg tggattttat gaaaagcttt ataatagcca atttgcagac
    10741 acagaatcag aatagataac ataaactttt gtatgaaatt ttgtatcaaa ttcaaatatt
    10801 aatcatataa ttaaaaagag tatcagtaag ttgtttttta tagatttact gatactcttt
    10861 gattttaaga ccaaaggtac ttatggttgg tacttataat tcgtaactag tcttgttaca
    10921 tctgataacc agaattacag tacttttctt ttttaccttt ttgttctcta acatataaca
    10981 attcgcttaa cttatcaatg taataaccat catcacttat acgaattaaa ctagtcagat
    11041 aatatccatc tttgcttcta ggaatctttt tatttattgc taaatcaata gctgtctttg
    11101 ctgcttcaaa atgctttaaa tgtgtatgac cttcttcaaa actctttcta gtattataaa
    11161 ctacataacc tttttttact tgaaaaacta taaattcttt tttttcatat actttattaa
    11221 acattcaatc agcccttctt gagatagttt tatagcataa cattagaagc ttctaaaaaa
    11281 ttttacaaag aacataaata ttttataaat tataaatcta aaactataat ttataaagat
    11341 aactttctga ttatttatat gataattcat atagattaaa agtaaattat ctttaggtta
    11401 aacaaaagta aaataaaaga ctagttataa tatttatatg aaaaagtaca aaatatagta
    11461 tatgaatata gaaaatttaa attatttatt tacagttttt atttccataa gtatatttac
    11521 cttcagatac cttgtcatca tgaaaatata ttctttcaca acttttatct ccccataaaa
    11581 tgttttttgt tattttatgg gaggtatctc ctgtaaatac ttcagctaca gtagtatctg
    11641 taacatcaca ataaagagaa gcaaactttt taaaccaagg attttcctca aatcttaaaa
    11701 gccaggaagc tccataaggg caatgcaact cttcaaattc aggaacccat cctagaaatg
    11761 gaacatcagt tgtgctaata atattgtctg caattaattc atctccattt tcatgagctc
    11821 tttctctcat agcaatagct ctttcttcag acctttcaaa taaaactttt tttaatattt
    11881 cgctagtttt atcttcacca aactcttcaa ataacacttt ggaaaaataa aaatataagt
    11941 caccaaaatg cttgcatgcc atacgaactt cttcaatagc ataatctttg tcgatagttt
    12001 cattagttac aacatcttcg ttcaaaaaaa tcactcctta gaatagtatt agttacaata
    12061 taattataga atatttttaa taaattagga aattaattta agatatatat ttttttagta
    12121 aataaaagta aaatgctaaa ataacttatt gttacagaaa tcttctaaat taatgtattt
    12181 aggagtttcc atatgaatta gagtagtttt gtaaatatcc tcaatacatc catttgcaat
    12241 attattaaaa atatctgtgt gttcttttaa tgttgtttca agtcttccct catgtgacaa
    12301 tgaatcgaga gctaattttt ttattctgta catataattg ttaaacattt tgtcaaattc
    12361 agtattttga gcatatgaaa ctatagttgt gtgaaataat gtatcgtatt ctgcaaatga
    12421 gtttaaattt ttatcaccca gtaaaatatc tttttgttta tctaaaaggt gttttaattt
    12481 acttatagtt tctattgccc tagcttcttt gtattgactt gtaattaaaa aagtgcaaaa
    12541 cccttctatt gcagagcgta tttggaatat ctctataatt tcatttgctg taatttgatg
    12601 gattctaaat cctttgcttg ggattatatc tataaatcca tcttgactaa gtctttgaag
    12661 agcatccctt ataggtgttc tagagatgcc tatttcacta gcaagttttg tttcagaata
    12721 tatttcattt tctattattt ctccagatag tatcaattct tttaagtagt catatgcttg
    12781 ttcttgtaat cttttaaatg tagccatact taataatacc cccctaaaca atataaaaat
    12841 acaaaaactt atataatttt aagcatacaa catagtaaaa ataaggtcaa tcttttgaaa
    12901 tttaaattgc atataagatt aaaaaatata aaaaattgtt gaagtgtgat atatgattaa
    12961 cactaaagtc agattaatta aaattagttc aaatacaaaa ctgtaaaagt agtatatgat
    13021 atttaaaaaa tagtgtatat taaaacttat atagaataac taaaaataat aaataacttt
    13081 aaaaattact atttattgtt aaaataaata cttttatatt ttatattgac cggtatactg
    13141 gtatgtagta tactctaaaa cataaaaaac gttttcaaaa atgtgaagat atggaggtct
    13201 ataaaatgaa attaggattt attggattag gaataatggg aaagccaatg gcaaaaaatt
    13261 tattgaaaga tggatgtaat cttttggtgt atgacattga taaatctgct gtggatgaac
    13321 ttatatcatg tggagcaaag tatgcaagtg ttttagaaat gggtcgagaa tgtgatattg
    13381 tatttacaat ccttccaaat ggaactattg tgcaggatgt attatttgga gcagatggat
    13441 tggcaaaaac tttaaaagaa ggaagtatag tagttgatat gagttcagtt actcctacag
    13501 aatccgtatt gtgtgcaaat aaattaaaag agaggggctt agaatttata gattcaccag
    13561 taagtggagg ggagccaaaa gcgattgatg gtacgcttgc ttttatggct ggtggtaagg
    13621 aagaaattta taagaaagta gagccattct ttaatataat gggaagttca tctcttttaa
    13681 ttggagataa tggtagtgga agtgttacaa aacttacaaa tcaagtaata gttaatttga
    13741 cgatagcggc agtctctgag gcatttgtac ttgcggcaaa agctggtgca gacccagaaa
    13801 aagtatataa ggcaataaga ggggggctag ctggtagtac aatattggat gcaaagatac
    13861 caatgattat gaatcgtgat tttaaaccag gtggaaaaat atctattaat ttgaaggata
    13921 ttaagaatgt aatgcaaaca gcacataatt tagatgtacc actacctatg acaagtcaat
    13981 tattagaaat tatgcagaca ttaaaagtac atggacattt ggaagatgac catagtggaa
    14041 tagcacagta ttttgaaaag ttagcaggtg ttgaaataaa aaaacacaat aattaatact
    14101 taaatatatc aatataacta aatacatact attaggatta gattatataa atacagcaag
    14161 gttaaattat catattaaaa gaaaaataag attgataaat aataaagtag aatgataaag
    14221 gggtaaagat tatgattaca ggatatggac ttttggtagc atttgttata tctataggaa
    14281 ttttacttgt atctattatt aaatttaaag taaatccatt tttggcatta cttataacat
    14341 ctataattac aggctttatg gttagaatgc ctataaatga aatctcaacc acaatatcga
    14401 ctggatttgg aaatacatta gggagtatag gtattgtaat tggacttgga ataatattcg
    14461 gaaatattct ttcagaatca agagcaacag aatctattgc aaaggggttg ttagctaaga
    14521 ctggagaaaa aaacagtgct ttagcagtaa ctacagcagg ttttttaatt tcaattccag
    14581 tttttatgga tgcagctttc gttataatga tgcctataat taagtatgtt tcgagagtaa
    14641 ctaagaaatc tctaatggtt tttgtatgtg cattaggagt tgggactata gtaggtcatg
    14701 cacttgtaat acctacacca ggacctttgg cagttgcagc aaatgtaaat gcaaatgtag
    14761 gtagttttat actttattct ataattgttg cttttccagc agctcttata ggtggatgga
    14821 tttatggaaa acgttttgaa aaatatccag cttatgctat tgatgagaat gatagagaaa
    14881 aaaatttaga acagggaaaa gatagcataa aaattgaaga tgatgattct tctaaagtac
    14941 caggttttag aatttctatg ttttctttat tgttcccaat acttcttata cttatatcaa
    15001 atgtatttag tatgttctta gaaaaaggtt ctacaatgag tggagtgctt gcatttatag
    15061 gagataaaaa catagcaata cttcttggaa tattggtggc aataggattt ttaaagaaat
    15121 acataaataa acctatggga gatgtagtta ttgaagctgc tgattcagca gggctaatat
    15181 tattgataac aggttcaggt ggtgcgtttg gcagtgttat aaatgcaagt ggtataggta
    15241 acttcttagt tgatactatg tctggtctta gtatatcagt tgtagtgctt ggatttttat
    15301 taagtgcctt gcttagaatt tctcaaggtt ctgctactgt agccttagta acaacatcat
    15361 caattttagg acctacaatt ttggcaactg gaatgtcacc agttttagta gggcttgcta
    15421 tatgtgctgg aggagttggt ttctcccttc caaatgattc tggattctgg gtactttcaa
    15481 gatttagtgg attatcagtt aaagatactt taaactcttg gacaattgga ggaaccattg
    15541 ctggagttgt agcatttata atggttttat tgcttagtgt tataaatgga attataccac
    15601 ttccaggtct ataaataaca aactattgtt tatattatat aaaattacat ttagactata
    15661 caaattacat aaaggggcgt tttaaatgag agaagtaaaa ccaattagca tagatatact
    15721 taatactttt aaacaagtag atgaggacag attaaataaa cttcttgcag atgagcttaa
    15781 gcatttagat agaaaaattg ttgtattaga tgatgaccca acaggtgtac aaacagttca
    15841 tgatatttct gtttatactg attgggataa ggatagtatg gaacaaggtt ttaatgagaa
    15901 aaatagtatg ttttttatac ttactaattc aaggggattt actgttgctc aaactacaaa
    15961 agcgcataaa gaaatttcaa agaatattgt ggatgtatca aagaaagtta ataaggattt
    16021 tataatcata agtcgtagtg attctactat gagaggacat tatcctgttg aaactaattt
    16081 attaaaaagc gaagtagaga gattatcaga ga
//
LOCUS       DAMJVM010000076        15993 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.079, whole genome shotgun sequence.
ACCESSION   DAMJVM010000076 DAMJVM010000000
VERSION     DAMJVM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15993
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.079"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            167..805
                     /locus_tag="PC523_002624"
     CDS             167..805
                     /locus_tag="PC523_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2476131.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            812..1654
                     /locus_tag="PC523_002625"
     CDS             812..1654
                     /locus_tag="PC523_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDF2476132.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGILQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            1715..4342
                     /locus_tag="PC523_002626"
     CDS             1715..4342
                     /locus_tag="PC523_002626"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDF2476133.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRKEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDNALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGVEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            4556..4801
                     /locus_tag="PC523_002627"
     CDS             4556..4801
                     /locus_tag="PC523_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2476134.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKR"
     gene            complement(5091..6401)
                     /locus_tag="PC523_002628"
     CDS             complement(5091..6401)
                     /locus_tag="PC523_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDF2476135.1"
                     /translation="MNKKVSKIVAIGCGVGIITANLIPVYADNSTSSINKVDNSIVSD
                     STKRTSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFP
                     YDQIGLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMK
                     LKKDLGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEY
                     RFESFRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVA
                     PTKPEQGNLTDDEYKVLMDKYGKDYDRYKKEVEEYNLERQTYAAGLTTYANYLQQKYN
                     TENGLVTLEDNKKALKKGLIDSYAQLLALEDTIQITKKQLELSEKQLKNTKLRYDLGL
                     ITLTDYKKQVVSNEDAKNNYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(6423..9557)
                     /locus_tag="PC523_002629"
     CDS             complement(6423..9557)
                     /locus_tag="PC523_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDF2476136.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQIGQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     NNLLISSVIAFIVIFAFLKSVRASLVVAIAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSIDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTDGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDTPLFDK
                     VSELYKRLLSVCLRHQRLVVISSLLIFIASLFGMSFIGMDFMPAADKGELAISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNNTANIAIELESQKNRKRSTDE
                     VEKEIIDRVATVPDCKINVSQNSSVMGGTSGADFTLEMKGPDLDVLKILGKEAEKQFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGINTAEIAGLLRTAINGDSVTTATIDD
                     YKVDVNLKFKADSIDNLEDIKQLKVNSTTGKEVPLGAFSDIKMADGLQSISRTDGNFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGQTEMMNESMASLGFSMLIAI
                     VLVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTISTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGDI
                     KRKIIKKPKLKAKV"
     gene            complement(9561..10784)
                     /locus_tag="PC523_002630"
     CDS             complement(9561..10784)
                     /locus_tag="PC523_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDF2476137.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEEPEPEKPIAVSVQKAVGGEIE
                     NTNAFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPDLENSVKQSK
                     AALDLAKASYSSTTGGSLEAQVNQAKTALDNAKIQYDESQRNYDNNKILYEQEVISLD
                     QFKKIEFALEQSKQQLDSAQRSYDTVTSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTAPVDGTITAKNFDAKEMITQSQPAFIISNPNILEVNLNVAESDIDKFKKDGNVE
                     VIIEDQRILGKIDYVPSVVDPQTSLYPIKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEEKGKKYVYIANKDNIAKKKLVQTGIVTEDKIEIKSGVSEKDTVVIGGIS
                     LISDGTKIFPVEKEK"
     gene            11036..11521
                     /locus_tag="PC523_002631"
     CDS             11036..11521
                     /locus_tag="PC523_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2476138.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFRESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFELFGAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            complement(11632..13860)
                     /locus_tag="PC523_002632"
     CDS             complement(11632..13860)
                     /locus_tag="PC523_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HDF2476139.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFIPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAETGEYKGKYIKGKESKFDTLEDANKIIASIASKTGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKNLSKDEQLVYDA
                     IKDRFIANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKINQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEEYTFDSKTKKFTKTKEKKQKKDTTNA
                     EKEVAISKDSNNKKESKNGDENQPLGKCPVCQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDALLKYNKNPDTGYFNWKIEF
                     DN"
     gene            13973..14404
                     /gene="dut"
                     /locus_tag="PC523_002633"
     CDS             13973..14404
                     /gene="dut"
                     /locus_tag="PC523_002633"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDF2476140.1"
                     /translation="MYTLKVKKINDDAIIPNFAHKGDAGMDLYSIEEVVIPSGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIIKPIYDINIEEVEELSDSERGKGGFGSTGF"
     gene            14664..15854
                     /locus_tag="PC523_002634"
     CDS             14664..15854
                     /locus_tag="PC523_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2476141.1"
                     /translation="MNFNQKRIAASVMATAIIIPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKDGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTSENTGNSSNDIVKGTKVVTAKSLNLRTGPGTSHSKIATL
                     SYGTEVGRISENGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGAESVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSAGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLIFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
BASE COUNT         5552 a         2275 c         2529 g         5637 t
ORIGIN      
        1 taaagaaata tcccccatta atatataata aatatcttta agcgagagaa cttagtaaaa
       61 aatatatcgt attcaataaa aaatctgttt acagaatggt atatatagtt atataatata
      121 ctatataaga tgcgaaaaga taaaaagtat agcacaatga aagaaggtga ttattattca
      181 gcttaatgaa agacaactaa aaattataga tatagttaag gaaaatgaac caataacgag
      241 tgaaagtatt gcctccagct tgaatgtgac tcgtgctaca cttagatctg atttagctat
      301 attaacaatg acaggaatat tagacgcaag accaaaagtt ggatattttt attcaggagt
      361 tagtgaaata aatttaattg gtaagagtgt aaaagaaaaa acagtagaag atataatgag
      421 catgcctgta ctagctaaga aagacgaaag tatatatgat gtaatagtca ctatgttttt
      481 atctgatgta gggagtatag ttattataga tgagaatgaa gagttatgtg gagttgtttc
      541 cagaaaagac ttgttaaaag caactatagg aggttctgat ataaataaga tgccaatagg
      601 tatgataatg acaagaactc caaatgtagt aactttaact aaaggagcaa gtgttttatt
      661 agcttcaaga aaaataatag aacatgaagt agattcgatt ccaattgttg aatataaggg
      721 agaagacaaa aatcatatga gagttgttgg gagaatatca aaaaccaaca taactaagct
      781 attcttagaa atagttgaca attaaggagg tatgctggtg aaaaacctaa ttatatatgc
      841 agtgtcagat tcagtagggg agactgcaca acaagttgca aaagcatgta tgtctcagtt
      901 ttatgtgaat gaaacttatg aaataaaaag atttccatac atgattaata aaggagtact
      961 acttgaaact ttggagaatg caaaagctga aaacgctttg attgtatata cattagttga
     1021 tgaagaatta tgcagtattg ttgaaagata ttgcgaaaga gaaggactta gttgcataga
     1081 cttaatgaca gatatactaa gagaaataag caagagaaca ggaagaaaac ctaaaagaga
     1141 agctggtata attagaaaat tagacgaatc ttattttaag agagttgaag ccatagagtt
     1201 tgcagtaaaa tatgatgatg gaaaagaccc tagaggtatt cttcaagcag acataatttt
     1261 agttggaatc tcaagaacgt ctaaaactcc tttgagtatg tatttagcta ataaaaatat
     1321 aaaagtagct aatgttccat tagttccaga aataccaatt cctaaagaag tgtttgagat
     1381 agatactaag aagataatag gtcttactaa ttcacctgaa aagctgaatg agataagaac
     1441 tcaaagatta aaagccttag gtttatctag taaagcaaac tatgctaatt tagaaagaat
     1501 acttcaggaa ttagattatt cagaagaaat aatgaaaaga attggatgcc cagtaataaa
     1561 tgtttcaaat aaggcaatag aagaaacagc aggtataatt ttagacataa tgaaagaaaa
     1621 tggtctaaag atatacaaag aaatagaaat ttaataatgt ctattaataa aaatgatata
     1681 aattaaaata aaaagattaa tgggggattt aacaatggaa actaagtatg tttatagttt
     1741 tggtgaagga agcaaagaca tgaaatcttt actaggaggt aaaggtgcca atttagcaga
     1801 aatgactaag ataggcttac ctgtccctcc tggattcact ataacaacag aagcatgtaa
     1861 tgattattat gttaacaatg aaagtataag aaaagagata ataaaagaaa tagaggagca
     1921 tttagctact ttagaaaaag atttaaataa aacattagga tgcaataaga atccattatt
     1981 agtatcagtt cgttctggtg cagtattctc tatgccagga atgatggata caattcttaa
     2041 cctaggtctt aatgacaaca gtgttgttgg tctagctgag gctacacaaa atgaaagatt
     2101 tgcatatgat agttacagaa gattcataca aatgttttct gatgttgcta tggaagttcc
     2161 aaaatataaa tttgaaaatg tattagacag agtaaaagaa gcaaaaggat atacagttga
     2221 tacagagctt acaactgatg atttaaaaga aatagtaaaa gaatttaaag caatttacaa
     2281 aaaagaaata aaaaatgatt tcccacaaga cccaaaagag caattaatgc ttgcaataga
     2341 ggctgtattt agatcatgga ataatcctcg tgcaatcgta taccgtaaat tgaatgatat
     2401 agctcataac ttaggaacag cagtaaacat tcaatcaatg gtatttggaa atatgggaga
     2461 aactagtggt acaggtgttg catttactag aaatccagct acaggagaaa ataaattatt
     2521 tggtgaattt ttaatgaatg cacaaggtga agacgttgtt gcaggtataa gaactcctca
     2581 aaatatatca actcttgcag acgtaatgcc agctgtattt gatgaatttg taaaaattac
     2641 tcatatactt gaaggtcact ataaagatat gcaagatata gagtttacta tagaaaatga
     2701 aagattatat atattacaaa ctagaaatgg taagagaaca gcagcagcag caataaatgt
     2761 agcagttgat ttagttgaag ctggaataat tgatgaaaaa gaagctataa tgagaattga
     2821 acctaatcaa ttagaccaat tattacatcc aaaatttgaa gataatgcgt taaaagaagc
     2881 taaggttata gcaaaaggtt taccagcatc accaggtgca gcaagtggta aagtttattt
     2941 caatgctgat gatgtagtaa aagcaaatga aaaaggagaa aaagtagttt tagttagact
     3001 agagacatct ccagaagata tagaaggaat ggttaaagca gaaggaatcc ttacagctag
     3061 aggtggaatg acatcacatg cagcagttgt tgctagaggt atgggtaaat gttgtgttgc
     3121 tggatgtgga gaaataaaag ttgatgagtt taataaagaa ataagagcac ttgatgatgt
     3181 agttataaaa gaaggagaat atatttctat agatggttct acaggaaatg tgtacctagg
     3241 agatgttaag aaaacagaag tatctttaac tggaaacttt gaaaaattaa tgaactgggt
     3301 ggataaacac aagtgtatga tggttagaac taatgcagat aatcctagag atgctcgtgc
     3361 agctatagaa tttggtgctg aaggtatagg tttatgtaga actgagcata tgttctttga
     3421 tgaagctaga ttgccagcag ttagagaaat gatattatct aacacagtag aacaaagaga
     3481 aaaagctctt gaaaaaatat taccaatgca aagagaggat tttgtagagt tatttaaagt
     3541 tatggatgga aagccagtta atataagatt attagaccca ccactacatg agttcttacc
     3601 tcatgatgat gagactatag aagaactttc aaaatctatg ggaataaaag taagtgatat
     3661 taagaagaga atagttgatt tagatgagtt taacccaatg cttggacaca gaggttgtag
     3721 acttgcaata acttatcctg aaatatgtgt aatgcaatct aaagctataa tacaaggtgc
     3781 tatagaagca atcaaagcag gggttaaagt ttctccagag ataatggttc cattagttgg
     3841 agaagtaaat gagttaaaaa taataagaaa aatgatagta gaaactgttg atgctataat
     3901 aaaagaagaa ggcgtagaag ttccttatac tgtaggtact atgatagaaa tacctagagc
     3961 ttgtttaact gctgatgaga tagcacaaga agcagacttc ttcagttttg gtactaatga
     4021 cttaactcaa atggcatttg gatattcaag agatgatgct ggtaaattct taggtcaata
     4081 tgtagatgaa gagatattag aaaaagaccc attccaagta ctagaccaaa atggtgttgg
     4141 taagttagtt aagatgggag ctaaattagg tagagaagtt aaaccagaat taaaattagg
     4201 aatatgtgga gagcatggag gagaaccatc ttcagtagag ttctgttata gtgtaggact
     4261 taactatgta tcttgttcac catttagagt gccaattgct agacttgcag cggctcaagc
     4321 atctataaaa aatcctagat aataatagaa tgtaaaatat gtaactaaat aaaaataata
     4381 tcccctattg ttcaatgtat caaatctagt atttgtaagt tgaatatagg ggatttttat
     4441 tataaaaatt taacttatat ggttgtaagt gaaacgtatt taattatatt gcatatatta
     4501 ttgtagagag aaattttaac tattaattaa attgagttta ctggagggga taaagttgac
     4561 aaagtgtcct aaatgtggaa aggaagtatc atcacgtcca ggtagtatat gcccacgatg
     4621 tggatttaag gtagcagatg agagtctaaa aataagatgt ccagaaccta gttgtagggc
     4681 tttggtatct aggaaactgg attattgtcc taaatgtgga tgcaaactta gaggatataa
     4741 tatttctgaa tttgttaaca tggtgagatg taaaatagat gagacatttg ataaaagata
     4801 attaaattta aagttaaact tttgagggtg tctcaaaatg aactttttag tttatgagac
     4861 actctttttt gtatgaaatc aaatatattt ttatcattta aatagttaaa aagaggctta
     4921 tctccatttt gagatagcct ctataaaagt atacagtttt ataagtaaat atatttttgc
     4981 taatctattt ctattatttg ccttatgaat cttccatagc taaagtcata agtgtgcata
     5041 gctatttttt aatcaaatgt atgttaaagt aatctttcta tttaccagaa ttactttcca
     5101 gtattaagta tccaaggttt ctctatacca ttttttagtg agttataatt cacaataagt
     5161 gtgtcatagt tatttttagc atcttcattg cttacaactt gttttttata atcagttaga
     5221 gtaattaatc ctaaatcata tcttaattta gtatttttta attgtttttc tgatagttca
     5281 agttgttttt tagtaatctg aattgtatct tctaaagcaa gtaattgagc ataactgtca
     5341 attaatcctt ttttaagagc ttttttattg tcttctaacg ttactagacc attttcagta
     5401 ttgtattttt gttgaagata attcgcataa gtagtaagac cagcagcata agtctgtctt
     5461 tcaagattgt attcttcaac ttctttttta tatctatcat aatcttttcc atacttatcc
     5521 ataagaacct tgtattcatc atcagttaga ttaccttgtt ctggttttgt aggagcaact
     5581 ggtcttgtaa gattaggtct atctggtaaa tctgctttgt tctcatcttt atcattaaaa
     5641 ctttcttctg tacgttctaa aatcaattgg tcgtatttta aatattcatt aaccttgcct
     5701 tccatatatg aatcaactga gccacctact ctgaaagatt caaatctata ttctttatct
     5761 aattgataat tatttaaatc tatattagtc aataatttaa aatagtcttg attatttttt
     5821 agttcttgaa gcttagcttt ttgagtattt tgtagtgttt gaagttcaag ttcagctgac
     5881 tcagtttcta aagatgttac tagccctaaa tcttttttta atttcatatc tttaatttct
     5941 ttagttttta tctcaagatt attttttatc ttatctaact cattttctct tgaaactaaa
     6001 tcgttaaatt tattagtaat atcattggct atttggtcct ccataaaacc tttagcctgt
     6061 tcactttgat ttttaagaag tccaatttgg tcatatggaa atgagttgtc acttccagta
     6121 aaactatcaa attcatcttg cacttctaac ttttctttta acatttttat ttcttctgat
     6181 ttcattttta atttttcact atttttaagt gcagcactaa cagcttctct agcagttaaa
     6241 atttcctttt cagatgttct tttagttgag tcactcacaa tggaattatc aactttattt
     6301 atagaagaag tactattatc tgcataaacg ggtattagat tagctgttat tataccaacg
     6361 ccacagccaa ttgcaactat tttgctaact tttttattca atacaatcac cctccattct
     6421 atttaaactt tagcttttaa ttttggtttc tttattattt ttctttttat atcacctatt
     6481 ttatctgtta ttttaccaaa tatcttaggc attttgtctg aaaacttatt ttcccattta
     6541 tcaaatatca agtatatagt aggtataagt accaaagtta taagtgtaga tatagtcaga
     6601 cctcccatta taactattgc cagtggttgc attgtttctc caccttcacc aagagatagt
     6661 gccgttggta acatacctaa aattgttgtt gctgtagtca ttaggactgg tcttaatctt
     6721 gctgcacaac caattgaaac caaaccaatt aaatcattat tacgatttgt ttttctaagt
     6781 tgctctatgt agtcaataag aactatacca ttgttaacaa caatacctat aagtaagatt
     6841 atacctagca tacctacagt acttaaactt tgtccactaa ctagaagaga aagcacaact
     6901 ccaacgaatg caaatgggac acagaacata attataaatg gtttactaaa tgattcaaac
     6961 tgagctacca ttaccatata aactaatact atagctatga gcattgaaaa tccaagactc
     7021 gccatagatt cattcatcat ctctgtttga ccaccaacac caatagaata atctttagga
     7081 attaccattt tattcgtttt ttctagtgct agttttgtag cagtaccagc atctaaattg
     7141 tcaagtgtag cagttatatt tacagagaaa tttccatcag ttctagatat agactgtaaa
     7201 ccatctgcca tttttatatc tgaaaaagca ccaagaggaa cttcttttcc agttgtagag
     7261 tttactttta attgttttat gtcttctaga ttatcaatac tatctgcttt aaattttaaa
     7321 tttacatcaa ccttgtaatc atcaatagtg gcagtagtaa ctgaatcacc atttattgct
     7381 gtacgcagta atcctgctat ttcagcagtg tttataccat attcttgagc tttgtgtttg
     7441 tctattgtaa attgagcttc ttgtgttgta tctgtaagag aagtctcaac atttctaaat
     7501 ccttttatag agctgaattg tttttcagct tcttttccta atattttcaa tacatccaaa
     7561 tcagggcctt tcatttctaa tgtgaaatct gctcctgaag ttccacccat gacactacta
     7621 ttttgactaa catttatttt acagtctggg acagtggcaa ctctatcaat tatttctttt
     7681 tctacctcat cagtacttct ctttctattt ttttgtgatt ctaattcaat tgctatattt
     7741 gctgtattat tatttgaatc tgatgtcaaa gttgttatta aagttttaat ctctggaata
     7801 tctgaaactt ttttttcagt catagataca tagtaatcat tagattctag actaagacct
     7861 tttggaagtt ctatagaaat agccagttca cctttatctg cggcaggcat aaagtccata
     7921 cctataaagc tcattccaaa taaagaagct ataaaaataa gtaaacttga tattactact
     7981 aagcgttgat gtcttaagca tacacttagt aatcttttat ataactcact tactttatca
     8041 aataaaggag tatctttttc ttgcattttt tgactattct tgcctctact taattttgca
     8101 aatacactag ggacaagagt aattgcacct atgaaagaga aggctaggga agctattagt
     8161 gttttaccta atgcaccaaa ggttattttg gctataccat ctgtaaacat aataggtaaa
     8221 aatattgcta tagtagtcaa tgttgatgca agtattgcat ttgttacagt tcttgttcct
     8281 tcaatagcac aatcatcaat actcaatcta ccgcttttcc tatatttaaa tatattttca
     8341 attacaactg tggcattatc aacaaccata ccaaccgaaa ttaccagtga acttgcagta
     8401 ataagattta aactttcacc agtaaagtag agaattgcta tagcacctat tatagaaatt
     8461 ggtattgcaa tcgcaacaac cagtgaagct cttacacttt tcaagaaagc aaagattaca
     8521 ataaatgcaa taacagaact tataagcaag ttgttcatta cactgtttat tgattcactt
     8581 atgtatgaac ttgtgtcgtt agcaatttta attttaaatt ggggattttc tttatttaat
     8641 tcatccacat atttgtatgc actttgcata acttttacag tattggcatc ttgttgttta
     8701 gaaatatcta ttaccaaact gtcttttcca ttgtatctgt aaatactatc tttgtctgca
     8761 tatccataat ctacactaca aagattatct aatctaactg tatttccacc ttttacagga
     8821 atttgaatct gttttatatc atctagcgat tccaacttat caattgctct taaaactatt
     8881 ttatcttcac cttgagtaat tgaaccataa gggaatgttt tattactaga ggatagtaca
     8941 ccttttatag tggataaact aactccataa tttgaaagaa cagctgggtc agctattaca
     9001 ttaacctgtg ctttttctcc acctttaagg tctgctgatg taacaccatc tatggattct
     9061 attttaggtt gaatcacatc ttcagcatat tttcttaatg cttgtgaact attttcattt
     9121 cctccagata taacaagttg acctatagct tgtgcattca tatccaactt taaaacagtt
     9181 ggtttagctg catcatctgg aagactagtt tgtaatgcat caactttaga cctaacatca
     9241 ttcattattt catctaaatt agttccaaat tcaaattgag ccactaccat agaaacactc
     9301 tcttgtgaac ttgatatagt agattttaca tttgaaacag ctgataaact ttcatcaact
     9361 ttttgaccaa tttgttcatc aacatcttga ggaccagcac ctgtccaagt agtcatgacc
     9421 attacaacgg gtatttctat atctggcatc agatttattg acattttctt atatccaata
     9481 ccaccaaaaa ctattaatac taaaaatacc attataattg tgagaggacg tttaacggaa
     9541 gtttgtgtta aattcatagc ctatttctcc ttttctacag gaaatatctt agttccatca
     9601 gaaataagac ttatcccccc aataacaact gtatcttttt cacttacacc acttttaatt
     9661 tctattttgt cctctgttac aattccagtt tgaactaatt ttttctttgc tatattatcc
     9721 ttatttgcta tgtatacata tttctttcct ttttcttcaa atatagcctt tttaggtact
     9781 gtaacggcac cattttcttt ttcaacactt aagtttactt gggcagacat tcctgcttta
     9841 aatttgttgt ttgtgttatt aacaagcact ttaataggat ataaagatgt ctgtgggtca
     9901 actactgaag gtacataatc tatttttcct aaaattcttt ggtcttcaat tataacttct
     9961 acatttccat cctttttaaa tttatctata tctgattctg ctacatttaa attaacttct
    10021 aatatgttag gattagaaat aataaaagct ggttgtgatt gtgttatcat ttcttttgca
    10081 tcaaaatttt tggctgttat tgtaccatct actggagctg ttaatgttaa tttatctaaa
    10141 ttactcatag ctaaattata tgatacttgt gcttggtcaa gttgtttttt agcaagagct
    10201 ttagcttgtg gtatactctt tgaagttact gtatcgtatg atctttgagc agaatctagt
    10261 tgttgctttg attgttctaa agcaaattct atttttttaa attggtctaa actaattact
    10321 tcctgctcat aaagaatctt attattgtca taattccttt gagattcatc atattgaatc
    10381 tttgcattat caagtgctgt ttttgcttga tttacttgtg cttctaatga accacccgtt
    10441 gtacttgaat aacttgcttt agccaaatct agtgctgctt tactttgttt tacactattt
    10501 tctagatctg gactgcttat actcaataat tcatctccct ttcttacatt ttgcccaagt
    10561 tttacataaa cttcttgaac tgtgccaaca gtctgtgcag taacagctgt ttcatcttta
    10621 acctttgtag ttcctgaaaa agcatttgta ttttcaatct ctcctcctac tgctttttga
    10681 acagaaactg ctattggttt ctcaggctca ggttcttctg tttttccaca tccaactaaa
    10741 aacagtgaac tacacattaa gaaaactatt aaatatcttt tcatatctcc tcctattata
    10801 tattgcacac taacttgttg tagaatttaa agtgtctcat aattcaagtt tatttttagg
    10861 ctaattaaaa aactttcttt tattggcaat ttataattct ctaatttgat tataaattta
    10921 taattattat gagtcaataa ctttttcttg ttttaaattt gttaatagtt tataatttat
    10981 attatataag taacaaatgt tttattacat catttattga agggatgtta caaatatgga
    11041 taataacaat atacttaaaa tagcagactc ttttttagac tttttatttg tgcttgaaaa
    11101 taatatattt agagaaagtg atttgttaaa aaaatttcaa aataactctg atataatgaa
    11161 agcgtatttt gaagaatgtc caatggctcc atcacatgct aaggtaattc tttatctcat
    11221 gacatcaaat tcatcatcaa tatctcaaat agcttctaat ttaggaattt taaaatcaaa
    11281 catgacaccg attattgaca gattagtgga acatggttta gtaaataaat ttcctgaccc
    11341 aaaagataga aggattctta gggttgaatt aacggataaa gcatttgaat tatttggcgc
    11401 agtaaaagct attctcaaag aatctctagt aaaaaaatta tctaatcttt ctgaggagga
    11461 actaacttta ttagatgaac atacattaaa gttatcagaa atagtaaaga aattaggtta
    11521 gcatgtggtt gtaaaagtca ttattaaaaa acttaaaact tcataagaaa agaaatagca
    11581 gtagtataat atagaatatt atactactgc tatttctttt cttatgaata tttagttatc
    11641 aaactctatt ttccaattga aatagccagt atctggattt ttattatact ttaataaagc
    11701 atcaaattta ttaccatttt tagcagttaa agattttact ttagtttcac ctttttttaa
    11761 aatatttttg accattgttt tgttaacttt cttcttataa aggcttaaat acttgtcatt
    11821 tttccaaata acaaatttac attctttatg atttttacaa gcaaagcctt tgtcaaattc
    11881 caatacatca ccttgacaaa cagggcattt accaagaggt tggttttcat caccattttt
    11941 agattctttt ttgttgttag aatcttttga tatagcaact tctttttcag cattagttgt
    12001 atcttttttc tgttttttct cttttgtttt agtaaatttt ttagtttttg aatcgaaggt
    12061 atattcttct gtgtttatat tttttgaagc atcttgtttt ataatattga cattttgata
    12121 tataaaacta atgatattag taaggtattc ttttcttgta tactttcctt tttgaatatc
    12181 actcaaactt ttttcaagtt tcccggtata atctacatca aataagtctt taacaggaaa
    12241 aatttcaact aaatttttcc caaggtctgt tatagaataa gattttcctt ttttctttac
    12301 atagcctact tgatttatct tctttaatac atcagcacgt gtagctgatg ttccaatcga
    12361 atatccggac aacactgttg tgtcttcttc aggcacattt ttaccacaat ttttcatagc
    12421 ctttaaaaga gtgtcctcag tatacggttt aggtggagta gtttgcttag ttaatggttt
    12481 tatttctaac acatctacaa catcatcttt atttacaagt ggaagtaaat cgtttttttc
    12541 ttccttatta tatacttcaa gatatccttt tgatttaagc accttacctt ttgttaagaa
    12601 agtacaatta tctacttcag tcttaatttc tgtattctca tattcagcag gaggcataaa
    12661 gtttgctatg aatctatctt ttattgcatc ataaactaat tgttcatcct tagataagtt
    12721 ttttggaatt atataagttg gaattatagc actatgagag tctaccttag aagaatcaaa
    12781 gactctttta gttttagaaa atttaatttt attttcatat tcaagaccaa cctttaattt
    12841 atctagagtt tgagaagctt ttgaagccaa actttcttct aagaaaatag aatcagttct
    12901 agggtaagtt atgtatccac ccttaccatt accctcataa agagattgac agacatttaa
    12961 aaccttatca gatgtaaaat tggaatactt agaagttata tatccttgaa gtgatgttaa
    13021 actaaaaagt tttggtgcat attcttttga catagtaact tttttatcta gtattttacc
    13081 agttttagat gcaattgaag ctataatttt atttgcatcc tcaagtgtat caaatttact
    13141 ttctttgccc ttaatatatt tacctttgta ttcaccagtt tcggctttaa aatgaccctc
    13201 tatttcataa taagttttag gaacaaagtt tagtatttcc atatccctat catagactaa
    13261 tttaactgtt ggaagaatta ccctacctat gtttaatagt ttcccatttc catatttcag
    13321 tgtagcaaca gaagtgaaat ttattccaat caaccaatca gtaataagtc ttgtatatcc
    13381 agcagcttga agatttctca tctcatcttc gtcctttaag ttttttatac ctcttgtaat
    13441 gtcttctggt gtccattcat ttactaagat tctttttact ggttttttat tttttgcaag
    13501 taaaaatatt aaaaatgcta taagttctcc ttctcggtca ttatcagtag catttattac
    13561 atattccaca tcttttctat tcaaaagctt ttttacagtg ttgaattgtt tggttttaga
    13621 actatcaatt ttaaatttaa atttatcctc aggaataaat gggaaattgt tcattttcca
    13681 agagccagag tatttatctt tatcataatc cttcatatca tataatgata ctaagtgtcc
    13741 aactgcataa gtaattatat aatcatttcc ttcaaagtaa ccatcttgtc ttgtctttgc
    13801 acctaaaaat gatgctatag tttttgccac agatggtttc tcagcaagta caagtttcat
    13861 aaaaaatcct cctttaaaac actaataatt atattaccac aaaaaaataa aaaacttcaa
    13921 gaggtcgaaa tttaagttat aatatttata gaaagttgaa aggagaaata agatgtacac
    13981 tttaaaagta aaaaaaataa atgatgatgc tataatacct aatttcgctc ataaaggtga
    14041 tgccggtatg gatttgtatt ctatagagga ggttgtaata ccttctggag agactaaact
    14101 tatcaaaact gggatatgta tagaacttcc tactatgaca gaagcacagg taagaccgag
    14161 aagtgggctt gccttaaagc attctgttac tgtattaaat acaccaggaa ctattgatga
    14221 aggatataga ggagagctta aaattatatt aataaatcat ggtaaaaatg attttaaggt
    14281 tgaaaaacat atgaaaattg ctcaaatgat aataaaacct atctatgata ttaacataga
    14341 agaagtagaa gaattaagtg actctgaaag aggaaaaggt gggtttggtt caactggatt
    14401 ttaaaaagtt acaaaaaagt taaaaaatat agctatttta aaaaaaagca aaatatggct
    14461 atattgctaa attacaggct gttaaggatt aaaatttata catggtaatt tatggtaaac
    14521 aaatattact atagacaaat agttacaaat ttgtaataat attaatataa aagaaataat
    14581 tgatatttta agagtgtcaa ttatttcttt tatattttga aatgaaataa agctagtaga
    14641 aaagaggaga tgagtggaaa gttatgaatt ttaatcaaaa gagaatagca gctagtgtaa
    14701 tggcaactgc gataataata cctactatgg ggaatttagc atatgctaat gagtctgaag
    14761 tagaaagtgt gagtatagaa agcagaacaa taacagggaa tgcagtaaat tttagaaaag
    14821 gaccaggtac aaaccatgaa tcaatgggga aactttacaa aggtgataaa gttgagtatg
    14881 taggaaaaga tggttcttgg gtaaaagtta aatataatgg aaacacaggt tatgtgcatg
    14941 gaaattatgt agcaataaat agccttggaa gtagtaatga aagtagtgat acttcagtta
    15001 agtcgacaaa agttgtaaca gcaaaaggct taaattttag aactggacca agcacaagta
    15061 gttctaaaat atcaacacta ggatacggga cggaagtagg atacatatct gagtcaaatg
    15121 gatggtcaaa aataagttct aatggtagag tgggttatgt ttcaagtaaa tatttaggaa
    15181 ctagtgtaaa tgactctaca agtgaaaata ctggaaacag ctcaaatgac attgtaaaag
    15241 gtacaaaggt ggtaacagca aagtcattga accttagaac cggaccaggt acaagtcatt
    15301 ctaaaatagc tacattaagt tatggtactg aggttggtcg tatttctgaa aatggagggt
    15361 ggacaaaagt aagttatggc aatcaaacag gatatgtatc aagtcaatat cttgctgaaa
    15421 aaggttctgt tgatacttca attccatctt attcgactaa ttctccaagc caaggagctg
    15481 aaagtgtaat aagttttgca aagacactac ttggaaaacc atatgtgtgg ggagcagaag
    15541 gtccaaatag ttttgactgt tctggattca cacaatatgt tatgaaaaag tctgcaggag
    15601 ttagtatacc gagagtatct agagaccaaa gcaagtatgg aacatatgta aatagaggag
    15661 accttagaag tggagattta atattctttg atacacaagg ttcaaataat ggttctgtaa
    15721 gtcatgttgg aatatacata ggaaatggag atatgataca tgcatcttca ggttcatcaa
    15781 aaaaggtaac aatatctaat ataaatagta gttactattc aagtagatat gtaaatgcaa
    15841 gaagagtttt ataattattt tttattacaa aacctaataa acagattttt ctttattgct
    15901 tataatatga aagcaggtac aatattgata gtaatgtatg ttataataaa tattactatt
    15961 tgatttgtac ctgctttttt atttattttc tct
//
LOCUS       DAMJVM010000077        15831 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.080, whole genome shotgun sequence.
ACCESSION   DAMJVM010000077 DAMJVM010000000
VERSION     DAMJVM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15831
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.080"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(<1..2558)
                     /gene="carB"
                     /locus_tag="PC523_002635"
     CDS             complement(<1..2558)
                     /gene="carB"
                     /locus_tag="PC523_002635"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDF2476142.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIAENEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVAKELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGV"
     gene            complement(2611..3654)
                     /gene="carA"
                     /locus_tag="PC523_002636"
     CDS             complement(2611..3654)
                     /gene="carA"
                     /locus_tag="PC523_002636"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDF2476143.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKETIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     TENIKGTGAKVTVIDFGVKNSVLESLKACNCDITIVPAMTTAEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            complement(3680..4396)
                     /gene="pyrF"
                     /locus_tag="PC523_002637"
     CDS             complement(3680..4396)
                     /gene="pyrF"
                     /locus_tag="PC523_002637"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDF2476144.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDKLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVRAAVRDNVWLMTIHVSDMEGMRQCAL
                     VAKEEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKRGAHYLVVGRPI
                     TKAKDPRESAINIVREIENA"
     gene            complement(4772..5407)
                     /locus_tag="PC523_002638"
     CDS             complement(4772..5407)
                     /locus_tag="PC523_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HDF2476145.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     KKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKVFEHH
                     GFEVFSVICKNGAIPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEARVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            complement(5431..6345)
                     /locus_tag="PC523_002639"
     CDS             complement(5431..6345)
                     /locus_tag="PC523_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476146.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKSFEEDEEDDLDYLVGIKTEEEDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKKPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEEIQEENVSLEKEELQDE"
     gene            6947..8176
                     /locus_tag="PC523_002640"
     CDS             6947..8176
                     /locus_tag="PC523_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2476147.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLSDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNKNLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPEGHVWLSGASKDPNGVSFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDKYGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGDWAF"
     gene            complement(8381..10762)
                     /locus_tag="PC523_002641"
     CDS             complement(8381..10762)
                     /locus_tag="PC523_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HDF2476148.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGIQNPEKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSEYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVESPTVVGERLNPTGRKALQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLKSILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVDRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAIAMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNECSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKLDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDKI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(10734..11459)
                     /locus_tag="PC523_002642"
     CDS             complement(10734..11459)
                     /locus_tag="PC523_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HDF2476149.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKDTIDECVAIT
                     KNKINPRYLSRIYPIRINKDNIVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSDLTKGLIIDSCGTTAIEEVCDIVQDNVEKVFLKQNKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISDTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            complement(11449..11904)
                     /locus_tag="PC523_002643"
     CDS             complement(11449..11904)
                     /locus_tag="PC523_002643"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDF2476150.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            complement(12186..13367)
                     /locus_tag="PC523_002644"
     CDS             complement(12186..13367)
                     /locus_tag="PC523_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDF2476151.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLIVTSMYIIKSLYFNGAI
                     SVINQLVYQLRDFRSLIGESLFYPFLFTITSLAIYSIISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDNEKDDIDELAYKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVISQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGLYVDIVTEKKGDIAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHGGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            complement(13357..14049)
                     /locus_tag="PC523_002645"
     CDS             complement(13357..14049)
                     /locus_tag="PC523_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2476152.1"
                     /translation="MKNTILVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMVLDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIEVGAVIIDKEKHSVTVAGNDIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            complement(14183..14899)
                     /locus_tag="PC523_002646"
     CDS             complement(14183..14899)
                     /locus_tag="PC523_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2476153.1"
                     /translation="MKKFKLIVAIILVVFISVFISKDRLLKKDKEPTVIAQHYSKQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            15025..15759
                     /locus_tag="PC523_002647"
     CDS             15025..15759
                     /locus_tag="PC523_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis protein TtcA"
                     /protein_id="HDF2476154.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRKEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMIYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
BASE COUNT         4850 a         2858 c         1755 g         6368 t
ORIGIN      
        1 actccactta ctttgcttat atatggaact gttctactag ctctaggatt aacttcaatt
       61 atatataatt cattttgaaa ttctataaac tgtatattaa ccataccaag cacattaagg
      121 tctaaagcta tttttttagt atattctact attttttctt ttatttcatc agatatattt
      181 tgacttggat acattgttgt actatctcca gagtgaactc cagctctttc taaatgctcc
      241 attataccag gtattaatat atcttcctta tcacatatag cgtctacttc tatttctcta
      301 ccagttagat atttatctat aagtactgga ttcttatggt ctctatcaaa tgcatcttgt
      361 aggtattgag ataatttttc ttcgttataa gttatttcca taccttgacc accaagtaca
      421 taagatggtc taactaatac tggataacct aattccttag caacttcaat accttcgtta
      481 agtgaccata caccttttcc ttttggtcta tttatatcta gtctctctag taaatcatca
      541 aacttctctc tatcctctgc tgcatctata tcatcaaaat ctgttcccag tataggtata
      601 tttttttcat gtaagaactt agccaatttt atagctgtct gtccaccaaa ctgtagtatt
      661 acaccttctg gtttttcttt ttctattata cttaaaactt cttcttctgt taatggttca
      721 aaatataact tatctgatgt atcaaaatct gtacttacag tttcagggtt attgtttatg
      781 attatggttt ctatgtccat ttttcttaaa gattttacac aatgaactga acaataatca
      841 aattctatac cttgacctat tcttattgga ccagagccta gtactatcac ctttttctta
      901 tcactaacaa ctacctcatc atattgctca taagttgaat aatagtaagg tgatatagcg
      961 tctatctcac ctgcacatgt atcaaccatt ttatatgctg gcttgatatt gtatagactt
     1021 ctcaattcat ataatttttc tgggcttatc ttcattaagt cagatatacc tttatccgag
     1081 aatccctttt tctttaattc aagaagatag tctttagtta aatcttcaat tttcataact
     1141 tttaattttt cttcttgctc tactatccat ttgaatttat ttataaacca tttatctata
     1201 cctgtaattt gttctatcat ttctacttta tagcctcttc ttatcatttc agctaagtca
     1261 aataatctct catcatcagg cactacaact cttttcttta attcctcaat acttctctcc
     1321 tcagatggtg tgtgaaccaa tgaatatttt cctgtttcaa gtgaccttat ccctttaaga
     1381 atagcagcct caaagttact tcctatactc atgatttcac cagttgccat catcttagtg
     1441 cctaattttc tatttgcttt tttaaattta tcaaaaggcc attttggtat ttttgctacc
     1501 acataatcta tagttggttc aaaacaagca taagtttttt tagttactgc attttctatc
     1561 tcatctaaag tataacctaa agctattttt gacgaaactt tagctattgg atatcctgta
     1621 gcttttgatg ctaaagctga tgatctactt actcttggat ttatctctat aactgcatat
     1681 tcaaaactat gtgggttaag tgcaaattga acgttacatc caccttgaac ttctactgca
     1741 tttaatatat ctaaagatgc ttttttaagc attgcacatt ctttgttact taaagtttga
     1801 gttggagcaa caactatact atctcctgta tgaactccga ctgggtctat gttttccata
     1861 ttacatacaa ctatacaatt tccctttgaa tctcttatta cctcatattc tatttccttc
     1921 cagcctttta tactcttttc tattaatact tgtccaacag ggcttaattg tagtccatgt
     1981 gaaagtattt ctcttaattc ttcctcattt tcagctatac ctccaccagt tccacctagt
     2041 gtgtatgctg gtcttacaac tactggataa ccaattgtaa gtgcatagtc aagacctgct
     2101 tgtaaatctg taactatatc actaactatt acaggctgat ttatttcttt cattacttct
     2161 ctaaatatat ctctatcttc accttttttt atagattcta ctgaagtacc tattattttt
     2221 actccatatt tgtctagtat acctttttca taaagttcaa ctgctaagtt taatcctgtc
     2281 tgaccaccca tacctgcaag taaactatct ggtctttctt tttctattat tttttctata
     2341 aattctattg ttaatggttc tatatatatt ttatctgcaa tctctttatc agtcattata
     2401 gtagctggat tactatttat taatacaact tcaattcctt cctctttcaa agattggcaa
     2461 gcttgtgttc ctgaatagtc aaactctgca gcttgaccta ttattattgg tcctgaccct
     2521 aatactaaag ttttctttat actatctaat ttaggcataa atttttctcc ttatctaaat
     2581 ggtttttact atctgaaaat aatactaaat ctatattaat gctaagaatt tattaaatat
     2641 acaatctaaa tcttcttttg ttgtgtattc tggaatgtac tgaacactat atattggtaa
     2701 ttccttatgt ctcattcctt ctatagtatt gtcatttaaa tttatgtgag ttacctctgt
     2761 attttctggc atttctgaaa cattatatcc atggttttga gaagttataa atacctttcc
     2821 agtttctaaa catttaactg ggtgattgcc tcctctatgt ccaaatacaa gttttgaagt
     2881 ctttcctcct aatgccagtg ctaaaatttg atgtcctaaa cctattcctg tcaatggctt
     2941 tttgcctatt aattttttta tattttcaac tatatcattt atatcctctg gatttcctgg
     3001 tccattagat aataaaatta agtcaggatt tatatttaaa atttcttctg ctgtagtcat
     3061 tgctggtact atagttatat cacagttaca agctttaaga gactctagta cactattttt
     3121 aacaccaaag tctataactg taaccttagc accagtacct tttatatttt cagtttcttt
     3181 tcttgtaaca ttttttactg aatctttgtt acaatgctct tctaacttag actgaacatc
     3241 ttgtaatgat acattttcta aagttataat tcctctctta gttccattgt ttctaataat
     3301 tttagttaat gctcttgtat ctattccttc aagtcctatt accttatttt gctttagata
     3361 tgtgtctata tccatttcac atctaaaatt atttgggtta tcacttttct ctcttacaat
     3421 aagtcctttt acttgcacct tatttgattc aacatcttct aaatttattc catagtttcc
     3481 tataagtgga taagtcatag taactatttg tccataataa gttgcatctg tcaatagttc
     3541 tccatatcca gccattgaag tattaaatac aacttctcca atagtttcct ttagatatcc
     3601 aaatgctttt ccttcaaata ctgttccatc ttctaaaatt aatttcccct tcatataagc
     3661 tacctcccaa atattttatc taagcatttt ctatttctct tacaatatta atagctgatt
     3721 ctcttggatc ttttgctttt gttataggtc ttccaacaac taaataatga gcacctcttt
     3781 ttattgcatc tgatggagtc acaactcttt tttggtcccc aacctctgct gatttaggtc
     3841 ttattcctgg gcaaactgtt ataaaatcag ttccacatgc ttctactatt ttatctactt
     3901 cttgcgctga acaaacaacc ccatctattc ctgattcttt tgccagttta gctctcttta
     3961 tagctagctc ttctgtcgtc atattacatc ctatgtcctg taaatcttgg ttgcttaatg
     4021 aagttagaac tgttactcca actactaatg gcttttctat atttaatctt tctgcttctt
     4081 ctttagcaac tagtgcacat tgtctcattc cttccatgtc tgatacatgt attgtcatta
     4141 accaaacatt atctctgact gcagctctca ctgcactttt catagtattt ggtatatcgt
     4201 ggaatttaag gtctaaaaat atttttttgc ctttttcttt aagataatct atcgttttac
     4261 cttttgtggc aacatattgc tctaacccta ctttaaatat atctacacta tcttctaatt
     4321 tgtctattag ttctttagct ttatcaaatt cgtcagtatc aattgcaact atcaactttt
     4381 ctttaccatt tatcattttt acatctcctt tttaataaag cctaaatctt cataaaaaaa
     4441 tcctcacacc aaaagaggca caaggatttt atctatacat aactctattc attagctatg
     4501 agatatcttt cccagtctct ctggactagt ttaaaagact ttattatatt taaattttta
     4561 ttttatactc atcaaaaacc tctagaagat ttactagagg ttatatgcta acactatcta
     4621 taacccttta gtctctctgg actactttaa ggaatttagc tgtaatattt ttctttattt
     4681 atataatttt atgtatctat aagataactt aaagttttat cttttttata attaaaccac
     4741 taattgcttt ttatgtcaat aagtctttga attattcttc atctttatca aacaattttt
     4801 tctcaaaata tgattctgca ttatatattg cagcaactgg attatgccca gttactctat
     4861 ctttgacaac taaagttgtt ataggagctt ccatatattt catggctaaa gtatcatgac
     4921 caacacataa tccaagaatt acattaaggt caaccctagc ttcatttaaa aataaagctt
     4981 gtcctattgg attgcacatt acctcatctg aacattttga tattgtttga gatgcttcta
     5041 gtccaattgt actcttaggt attgccccat ttttacatat aacagaaaat acttcaaacc
     5101 catgatgttc aaatactttt tgtactgttt gcatttctct ttttagccca atacaaaatg
     5161 ctataccaat ctttttataa ccacactttt tagcaaactc tataatctct tttagtctag
     5221 tatattgaca atacccctct gcttcaacta gtccagcatt ataagctatt tttttattct
     5281 cttcttcttc ataaagttgc tttgcttttt gttgtagctc aatttctcta gttggacata
     5341 tcttcattaa tttatccata tttccattag aacagcctct aagattacat tttccacact
     5401 tatacatatc caatatcccc ttccaaaatt ttattcatct tgtaattctt ctttttctag
     5461 acttacattt tcttcttgga tttcttcttc tggtttcaca tgctcaacta cagttataaa
     5521 atatgatttt cctattttat tccttatagg aagtttttca tatgaaacat ctattttatt
     5581 ttttatacaa gctatatcaa aatgtcttaa ggctacacct aagctaacct tattcatatc
     5641 ttcacagtgt ttcttaggtt tcttttcaaa taaatgaaga cctcgttcat ctttatggaa
     5701 ataccaaggt tgagaatttt ttacagatgg agctcttctt gtcaattcta aaatatcttt
     5761 ccactttcta ttcctattta tcttatttat catatctttt actggctttc tatctggtct
     5821 agcatatcta gtcctaaaca actcttcatt ttttgctgga tatccaaatg ctattattat
     5881 ataaggattt tctattttgt tttcatcttc ttcttctgtt tttattccaa ctaaataatc
     5941 taagtcatct tcctcatctt cttcaaagct cttattccca atatctacaa attccaagat
     6001 atcttctctc tttaagtttc cttcaagcca acaggtagct attcccaatg ttgtaagacg
     6061 taaaactaca ccttctgtag caaaccctat attctgtaaa tagtcctcac ctttatctga
     6121 tgtgactact atataatgag gtgcctttac cttacattcc ttacccatca aaaaatgtat
     6181 tatatgacct ctatcaacaa catgtgcttt aatatttaaa tcttcattta agtattctaa
     6241 atttttacaa cttcttttta tttcttccat taatggcttt tttatcttct tactggaaaa
     6301 atttcttaca gattttctat aaaaaatggc ttcataaagc tccatttatg ccctcctaac
     6361 tgaactcttc tgacctataa actctataaa tttatataaa tactttatat tttttatagt
     6421 tttatttcct ttttcctcct gctctatttt gaaattcata agaatatcta ctctagttta
     6481 atatagcagt ctaaaggctt aaattctatc ttgtcaatgt ctatatatta gcacaatttt
     6541 tttatttgat tctctatgct aatttaaata acaacactag ctattacttt tataattaag
     6601 ctttgccaga aaaatttttt ttcatttata aatatctatc tcattattat attataagtt
     6661 tttttcggta ttcttgtata gcttttcact aaaaaagtat ttttatttct actttaatac
     6721 tattatcaac aaaaaacata ctgcttaaat aggtttgcta tcttcacaat ctgttttaat
     6781 taaaatatat ttttttattt ctcaatctat aaagtataaa tattttgaat atgacctaaa
     6841 atctaagtaa agttataata ttttataagt tgttcaatat taaaaacata agtaatgtta
     6901 taatacttta taagttattc aatattaaaa atataggagg ataaatatga atttaaatag
     6961 aaatttagaa aaatacgctg aattagcaat aaaagttgga gttaacatac agccaggtca
     7021 aattctactt ataaaatcac caatagaatg tgctgatttt gcaagaaatg cacttaagga
     7081 agcctataaa tgtggtgcta aaaatgtgta tatagagtgg tctgacgaag aaagcactct
     7141 tattaagtac ttatatgctc cagatgaggc atttcatgag ttcccaaagt ggacagcaga
     7201 acaatatgta gatatagcaa aagaaggtgg agcattttta tcagtctatg cccaaaatcc
     7261 agatttatta aaagatgttg accctgaaag agttgcaaat tttcaaaagg catctggaaa
     7321 agctttaaaa gaatggcgtt cttacactct aagtgataaa tgtaaatgga gtatagtctc
     7381 tgtgccaaca aaagattggg caaaaaaagt gtttccaaat ttatcagaag ataaggcaat
     7441 agaaaaattg tgggatgcta tatttaaatg ttcaagagta gatggtcaag accctataaa
     7501 agcatgggaa gaacataata aaaaccttaa atctaaaatg gactttctaa ataaaaataa
     7561 ctttaaaact ttaaaatata aatcctcaaa aactgatttg actttagact taccagaggg
     7621 tcatgtctgg ctaagtggag catccaaaga ccctaatgga gtaagtttta atccgaatat
     7681 tccaactgaa gagatttttg gaatgccaca taagtttaaa gtaaatggaa ctgtttatag
     7741 tacaaaacct cttgtttatg gtggaaacat aattgataat ttcttcttaa catttaaaga
     7801 tggaaaaata atagattttt ctgctgaaaa gggactagat acactagaaa aacttattga
     7861 aacagatgaa ggttctcatt atttaggaga agttgctctt gttccataca actcaccaat
     7921 atccaatacg gatataattt tttataatac tctatatgat gaaaatgcat cttgtcactt
     7981 tgcaattggt tctgcttata aaacttgtct agaaggtgga aacaatttaa aagatgaaga
     8041 attagataaa tatggtgtaa atgacagttt aacacatgta gattttatga ttggttctgc
     8101 tgatatggat attattggtg aaacttatga tggaaagcaa atacagattt ttaaaaatgg
     8161 tgattgggct ttttaacata tgattttaac taaaaagggc tgttggaagt tataccagac
     8221 ttcccagccc tttttaatgt tgatgataaa tattttttat attaattaat ctcattatta
     8281 tagattaatt cctattgttt taaacataag tatctaaaac ttatatttat attatatata
     8341 aaatatccta tttagtaaaa cctttatttt aatttacatg ctaaaaattt aactttgcta
     8401 tttcaacagc agactttgca tcctttgaat aataatcagc gcccatttct tcagcgtatt
     8461 cttctgttaa aactgcaccg cctacaaata cctttgcatt tatctcatta tctctcagtg
     8521 cctgaattgt atctttcata ctttgaacag ttgtagtcat aagtgcactt aatcctacta
     8581 attttatatt tcttttttta gcaacttcca ctacttcttc aatagggaca tctttaccta
     8641 aatctataac ctcatatcca taattttcta gcattatttt tactatattt ttcccaatat
     8701 cgtggatgtc tccttttact gtagcaacta ttatttttcc tttagataca ttattgctac
     8761 ttttacttaa taaagtttcc tttattgtat ttaatgaaac ttttaccgtt tctgctgatt
     8821 gaatcaattg tggtaaaaat atatctccac tatcatatct ttttcctact ttatcaagag
     8881 caggaattaa aatcttatct aatatataat tttcatcctt ctcttttaaa agattcagtg
     8941 ttacattttt tgcttcatct tttaatcccc tttcaactaa aatatccaag ggcaaatctt
     9001 tgtcaatctt tgtagatgaa tccaattttt ttgaattatt tatattacta tattgattta
     9061 tatagtttat acatccttta tctgtgttat ttaatatttt aaaagaattg atagcttcca
     9121 tcatactaca ttcatttgga tttataattg ctaaatcaag ccctgctcct aaagctaatg
     9181 ttagaaaaga agcattaagt gcttttctat tcggaagacc aaatgaaata tttgaaacac
     9241 ctaatatagt ttttactcct aatgtcttta ccattgctat ggcttttata gtttctattg
     9301 cttcttcttg ttgtgctgaa acagttaatg ataaacagtc tataaatatg tcttttggtt
     9361 ttatgccata agaaactgct ctatcaacta tcctttttgc tatttcaaat ctttcttcag
     9421 cctttttagg tatccccttt tcatcaagtg taagtccaac tacacacgaa ccatactttt
     9481 taactatcgg taatatgctt tttaaagact cttctttgcc atttacagag tttactattg
     9541 ttctaccatt ataatatcta agaccttgtt ctaaagcctc tacattggaa gaatcaactt
     9601 gtaaaggtgt atctactact gcttgaatct ctcttattaa ttttggcatt aatttttttt
     9661 catcaatctc tggaagacca acattcactc caagtatttg tgctccagca tttacttgtt
     9721 ctaagcctaa atttatagca taatcaacat tttcattttt taatgcttct tgtagagctt
     9781 ttctacctgt aggatttaat ctttctccaa caactgtagg tgattctact tcaacaaatt
     9841 ttgatggtga acaaactaca catttatcaa ttttctcgat acatcccttt gtaacagaat
     9901 ttatattctc agatatttct tttataaaac ttggattcgt accacaacat ccgcctatta
     9961 tagtagcacc aacctcaacg aactttttaa ccccttcaaa aaattctttt gcatcaacat
    10021 catatatagc ctctccatca actatatttg gaagacctgc atttgcttga accataacag
    10081 gtactgtagc tcttgatgct attttttcaa ctattggtaa aagttgttta ggtcctaatg
    10141 aacaattaac tcctattgca tctacaccta acccttctat agtagccacc atacattctg
    10201 gcatacatcc agtaaaactt cttccacctt catcaaatgt catagtacag aaaataggta
    10261 aatcagaatt ttcttttgct gctaatacag ctactttagc ttcatataaa tccatcatag
    10321 tctctattac tatcacatct actccagatt taacgccttg cagtacttgc cttttaaata
    10381 tttcatatgc cttatcaaaa cttaaagtac ccataggctc tagcatctct cctataggac
    10441 caatatctaa agctacatat cttggtttag tattatctac attttctata gctttcctag
    10501 ccaccaaaac agcattatca atcacttctt ctacagtata ctcactatct aattttagtt
    10561 cattacatcc aaaagtattt gtaagaatta catttgaacc tgcctctaag tatgctgtgt
    10621 gtatttctat aagtttctct ggattttgaa taccaaatac ttctggattt tcacctagct
    10681 ttaacccttt ttgttgaagc attgttccca ttgcaccatc gaatattaat atattatttt
    10741 tcagatattg tcttatttcc acaagagttc gctccttttc tatatgtaca gtttctatag
    10801 tttttacaat tttcacacga cttttctttc ttagatttat ttattcttgt atcagaaata
    10861 cctataatag ccactaccga ctttcttggt atcataattc cattagaagt aattgttaga
    10921 cctatttgtt tttgagcatt taaaacgttt aatatatcta tatttttttc tattggtaaa
    10981 tctccatatc ctggactata tcgcatagtt ataaatttat tttgttttaa aaaaaccttt
    11041 tctacattat cttgaacaat gtcacacact tcttctattg ctgttgttcc acatgaatca
    11101 attattagtc cttttgttaa atctgaataa gagtgtcgcc ttatttcttt ttctatatct
    11161 atccctatag tagctgccat aattacacat tcattacatt cttttaaaag ttcatataca
    11221 tctttactct ctaaagttaa atttgttccc tctaattcta caatattatc tttatttatt
    11281 ctaataggat aaatcctaga taaatatcta ggatttattt tatttttagt tatagctaca
    11341 cattcatcaa tagtatcttt aaggtcttta tctatctctt gacctctgta ttgtaaatat
    11401 cttaaaactt cactttgatt tatgcttata tcttctcttc caattaaatt attctccata
    11461 tatatttaaa gaaaatcctt tatctaaaac atcttttgca tattcctttg ctccaaataa
    11521 agataagtct ctgtagtttc cacattctat agctgtagct gctggcattt tatctgattc
    11581 aagaactttt tttaatgctt cttctaaacc aatcttaact gttttggcat ctacatctcc
    11641 ccatagtatt aaataaaatc ctgttctaca tcccattgga gataaatcta ttacaccatc
    11701 taatgtatct cttaaatagg tagccaaaag atgctctaaa ccgtgcatag cagcagttcc
    11761 aaaactttct acatttggct gtaaaaatct caagtcaaat ttagttactt tatctccctt
    11821 tactccatca agtacactac actttcttac aaaaggagct tttacttttg tatggtctag
    11881 cttaaaactt tctacttttt ccattattta atcctccatt atactttaaa atttgttaca
    11941 ttaattctaa aaaaaactct tcctatctat tattgataag aagagtatat tatttcaagt
    12001 catctcatcg ttgttagcag gaccacctga taaaaggttg gtataggatc actgagctaa
    12061 ctctctcccc taactcttga taagtatctt attaaactga cttaaattat atattacttt
    12121 tttatttttg taaacacttt attgttataa tatttatatc acgtaatcgt acttttattt
    12181 tataactaag ctgattgtat tattggcagt tgtacctcaa atacagtttg atatttatca
    12241 cttgaaacag taatttctcc accatgtaaa tttacaatat tttttgtaat tgcaagaccc
    12301 aacccagaac ctccaacatt tctatttctc gatttttcaa ctctataaaa tctatcaaat
    12361 atatttggta aatctaacac aggaattggt tctccataat ttatcacttt tacaaccgct
    12421 atatctccct ttttctctgt aactatatct acataaagtc catcttttcc atattttaca
    12481 gcattagtta taaggttttc aaatgctctt actaatttat tcggatcagc ttttacaatt
    12541 aatttttctt cattaaaatt aattctacta accataaatt cttgtttaaa ataaatttca
    12601 aattgagata ttacctgact taacaattct actaaattta tttctatttt gtttaatttt
    12661 attgtattat tctgcatttt agtcaattca aacaaatcat ttataagaac gttcaaattt
    12721 ttcgattttt catatgcaat atcaacataa tacatcatct gaacttcatc tttatactta
    12781 ccttcttcta taactcctaa ataacccatt atggatgtca aaggtgtcct taagtcatgt
    12841 gacacatttg ttattaaatc tgtttttgtc tgctgagctt gtctctcttc aatagttata
    12901 tttctaagtt gatgaacgat attattgatt ttatatgcta gttcatcgat atcatccttt
    12961 tcgttatctt ttaatatatc taagtcatat tcaatatcta tacttttttc taaattacct
    13021 tcatccatta aatttacagc actcttaatg atttctaatt tttgattttt tctattagtc
    13081 tttcttataa aatctacaat ttgcttattt ctcttgtacg atattatgga ataaattgct
    13141 aatgatgtga ttgtaaataa aaatggataa aataaactct ctcctataag acttctgaaa
    13201 tctcttaact gatatacaag ttgattaatt acagatatcg caccattgaa atataatgat
    13261 tttattatat acatacttgt tacaataagc attgaagtta aagcacagct tattataaga
    13321 tctaatatag gtcgaaacat atgatttaat aagcttttat ttttcaattt tatatcctac
    13381 tccccataca gtatgaatta ttttttctcc atctgtatat ttttcaattt tatcccttat
    13441 tctgctcata tgaaccatta cagtattatt agattggtaa tatttctctt tccacacttt
    13501 ttcaaatatt tcttctgtac tatatactct acctttattg tttgctagta aatataatat
    13561 atcaaattct cttgatgtca ggattatgtc atttccagct actgttacac tatgtttttc
    13621 cttatctata attactgctc caacttctat gacatcttta tcttcattat tagcaccact
    13681 aaaatagtat gctcttctaa tcagtgactt cactctcaca gtaagttcaa gtgggttaaa
    13741 tggttttgtc aaataatcat ctgcaccagt cattatccct tgtattttat ccatgtcttc
    13801 tgattttgca cttaacataa gtattggtat attcaaactt tctctaactt ttctacaaac
    13861 ttctattcca tccattttag gcatcattat atccaatacc attaaatgaa tattttcctt
    13921 ttttaaaata tctagagcct cttttccatc ttcagcttta attacattat atccactatt
    13981 taaaagatat atctctataa ggtctcttat ttccttttca tcatccacaa caagaattgt
    14041 attcttcact tttacacatc ctaacttaat ctttgtatat agttttattc atataaaatt
    14101 atactatatt ttagttagtg taatctataa atttattcat ttctaaattt ccaatataat
    14161 acaacttatt tacatattta tattatttgt tttgtaaata ttcttctaaa gttaatcctt
    14221 ttgaataaat ttcctcggca gcatccttac caacatatct aatatgccat ggctcatatg
    14281 ctgtacctgt tatatcttct ttaccttcag gatatcttat tataaatcca aacttgtgag
    14341 cattttctgc caaccattta gcttcttttg tacttccaac aaaccatctt tctggatttg
    14401 ttaaatcaat acaaagtcca gtttggtgct cactgtaacc aggctttgct acatatgcat
    14461 ctgccttttc tcgcccctga gattttaccc ttcttgtata agtatccagt tgagtatcat
    14521 aagatctgta tgctgaagac cctaaaaact gtattccttc tgaattagct tgcttaacta
    14581 aatcttcaac tgcctttgca ggttctcctg ccatttgttt ttcttcctct gttgcttctt
    14641 caacaaaagg tatgtttacc ttagtaatat tttcaggagt atagttttta ctaattccat
    14701 ttgtcttgtt taccaataat atatttgata tactatttga gtgattttta ttttgagaat
    14761 ttgcctcaac atcttgtttt gaataatgtt gagcaattac tgttggttct ttatctttct
    14821 taagtaatct atctttagat ataaatacac ttataaatac taccaaaata atagctacta
    14881 ttaatttaaa ttttttcata attaaatatt ctccttttca ttatgtttgt ctataatgtt
    14941 gattttagag tataagactt aagtatttta taagtaaatc ttaaataaat cttaaattta
    15001 atattattaa aggatggtga taaaatgtct atacaaaaac ttctaagtaa agctcgtaag
    15061 gctatacaag attttgatat gatacaagaa aatgataaaa ttgcagttgg actttctggt
    15121 ggaaaagata gtttaactct acttcatata cttaaaagtt atcaaaggtt ttctccacaa
    15181 aactttgaat tgatagccat aacattaaat actggaggtg ttgataattc tccactagat
    15241 aaattgtgta aagagataaa tgttcctttt tatgaatttc aaactgacat aaaagaaatc
    15301 gtatttgata taagaaaaga aaagaatcct tgtgcattat gtgctaatct aagaagaggt
    15361 gctttgaatg ataatgctaa aaaacttgga tgtaataaag tagctcttgg gcatcataaa
    15421 gatgatgcaa ttgagacatt tttaatgtct atgttttatg aaggcagagt aaattgtttt
    15481 tccccaaaga catacctaga tagacaggat ttgacggtta taagacctat gatttacata
    15541 gaagagtata tgactaaaaa aatctcaaaa gattctaact atcctattat aacaaatcca
    15601 tgccctgcaa atggtcatac tagaagagag tacataaaaa atcttattgc taatttaaat
    15661 aaagaaatgc ctgattttaa aagaaatgta tttggtgctc taaataattc tgagaaatta
    15721 tttatatggg ataaagagaa gataaaaaat tttaaatagt aattaaaatt tttaaataaa
    15781 aaagaaagat tgtattaatt gtaaataatc tttatataat ctacacaatc t
//
LOCUS       DAMJVM010000078        15827 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.081, whole genome shotgun sequence.
ACCESSION   DAMJVM010000078 DAMJVM010000000
VERSION     DAMJVM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15827
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.081"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            113..250
                     /locus_tag="PC523_002648"
     CDS             113..250
                     /locus_tag="PC523_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476155.1"
                     /translation="MSNQREDFDTTNDMFQESIPITTKIILDDIPSHDELNHVFSKGC
                     E"
     gene            254..751
                     /locus_tag="PC523_002649"
     CDS             254..751
                     /locus_tag="PC523_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476156.1"
                     /translation="MKKRKIVLITLLLIGVLLLGGILYNLFLVKTANISMLKESWNLD
                     IPIPNKEIEVFDTQDSINGDGQSYFIQEFSEKNFKKVFNLKGGIVVSKDNINEIEKYI
                     DKFKRDSVNINKSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYS
                     LIWNQ"
     gene            complement(1228..2586)
                     /gene="rlmD"
                     /locus_tag="PC523_002650"
     CDS             complement(1228..2586)
                     /gene="rlmD"
                     /locus_tag="PC523_002650"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDF2476157.1"
                     /translation="MLSKNEVYIVDIVDIGQGGVGVGKYNGITVFVEGGLIQDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGVPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGKENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYADLNETDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNMHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMSPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVAKII
                     KQ"
     gene            complement(2761..4521)
                     /gene="pyk"
                     /locus_tag="PC523_002651"
     CDS             complement(2761..4521)
                     /gene="pyk"
                     /locus_tag="PC523_002651"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDF2476158.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIICVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRVIKNGDTCVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSATDITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(4573..5532)
                     /gene="pfkA"
                     /locus_tag="PC523_002652"
     CDS             complement(4573..5532)
                     /gene="pfkA"
                     /locus_tag="PC523_002652"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDF2476159.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFVGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(5741..9310)
                     /locus_tag="PC523_002653"
     CDS             complement(5741..9310)
                     /locus_tag="PC523_002653"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDF2476160.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     FDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSDEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKIAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFLE
                     LEDLYGAIEIVVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFVDRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(9455..10408)
                     /gene="whiA"
                     /locus_tag="PC523_002654"
     CDS             complement(9455..10408)
                     /gene="whiA"
                     /locus_tag="PC523_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDF2476161.1"
                     /translation="MSFSTETKNELARVVSGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILENKEGFFTINKISDNLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKSSQ"
     gene            complement(10425..10850)
                     /locus_tag="PC523_002655"
     CDS             complement(10425..10850)
                     /locus_tag="PC523_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2476162.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(10919..12025)
                     /locus_tag="PC523_002656"
     CDS             complement(10919..12025)
                     /locus_tag="PC523_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDF2476163.1"
                     /translation="MAKLLIIVGILGWIALIISLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDKNLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(12037..12894)
                     /gene="rapZ"
                     /locus_tag="PC523_002657"
     CDS             complement(12037..12894)
                     /gene="rapZ"
                     /locus_tag="PC523_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDF2476164.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(12925..13638)
                     /locus_tag="PC523_002658"
     CDS             complement(12925..13638)
                     /locus_tag="PC523_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF2476165.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYNDINILLGMECNILDDKGNIDMDDKFIRYFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVANAISNNDIFIITHPGDKGDV
                     YIEEIAKTAKKTDTRLEINSSHSFLNLNQLNEIKGLENKFIIGSDAHCPERVGDFELA
                     IKTISDSNLDISLVENIII"
     gene            complement(13642..14556)
                     /gene="murB"
                     /locus_tag="PC523_002659"
     CDS             complement(13642..14556)
                     /gene="murB"
                     /locus_tag="PC523_002659"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDF2476166.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKKESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRVMDM
                     EGNIFELSNKQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(14829..15599)
                     /locus_tag="PC523_002660"
     CDS             complement(14829..15599)
                     /locus_tag="PC523_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF2476167.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMSTASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSMVVGGIYYLCYVKNS"
BASE COUNT         4888 a         3021 c         1701 g         6217 t
ORIGIN      
        1 attttttata taacttattc ttttataact tttaatttta aaagaggaga atgtatataa
       61 gaatattcaa tgtactagaa aaaatcaaaa gaaatattag aagaaatgga tattgagtaa
      121 tcaaagagaa gattttgata ctacaaatga tatgtttcag gaaagcattc ctatcacaac
      181 aaaaattatt cttgacgata taccttcaca tgatgaatta aatcatgttt tttcaaaagg
      241 ttgtgaatga aaaatgaaaa agagaaaaat tgtattaatc acattactat taataggtgt
      301 tttgttatta ggaggtatcc tatataatct ttttttagta aaaacagcta atatttcaat
      361 gctaaaggaa tcttggaatt tggatatacc aatacctaac aaagaaattg aagtatttga
      421 tactcaagat agtattaatg gagatggaca aagttatttt attcaagaat tttctgaaaa
      481 aaatttcaaa aaagttttta atttaaaagg tggtatagtt gtaagcaaag ataatattaa
      541 tgaaatagaa aaatatattg ataaatttaa aagagactct gttaacatta ataaaagtaa
      601 taaaaataaa attgaagaag attttaaaaa atataagtta gaagttaaaa aagatgataa
      661 gtatatatat aagagaaatt atgaaaacta tgtagtttta attataaaaa aagatgaaca
      721 aaaattatac agtttaatat ggaatcagta ggtacatatg tattattaag aattataaat
      781 aataaaaata aattttagtc aatatacaaa agccttttaa attgtctgga aatcacctct
      841 tcatttgtta taaaaacata caacaaatga agaggtgatt ttatggtttt taaaaaaata
      901 attataagtg gatatgtgct ttcacatgtt tatcaacatt tgtaatgcct atatttacta
      961 atgaattata atataaaaat attaaataat attcaactat agattatgta aattattcta
     1021 tgaaaaaaat gagttgtata aaatttggaa actgtagatt gacgatttct tttaaaatta
     1081 caattataat aagttctata gagttaaaaa taaaacttta gagattttta taacattgta
     1141 gactaaattt gccaccaaaa ttactttaca gtggcaaatc ggtggcaaat acaatatatt
     1201 attatcatat aacctatata aacattacta ttgcttgatt atcttagcta cagtttccac
     1261 atgagtagaa tgagggaaca tatctacagg ttgtgcctca acacatttaa atccgtgaac
     1321 atttagataa tctaaatctc ttgctaaagt agaaggatta caagaaacat agacaattct
     1381 ttccggactc attgaaacaa tagtctctaa taccttttcg tcacatccct ttctaggtgg
     1441 gtctacaact actacattag cagtcttacc ctctctatac atcttaggta caatttcctc
     1501 tgccttccca acataaaatt cagcattatg catattgttt aatttagcat ttatcttagc
     1561 atcttttatg gcatcatcta taatttctat tccataaacc tttttagcct tttgtgccaa
     1621 aaataaagat attgttccta tcccacagta aatatcaaaa acagtatcag tctcatttaa
     1681 atctgcatat tcaagagcct tatcatacaa tactttagtt tggacaggat ttacttggaa
     1741 aaatgataat ggcgaaatct caaacactaa gtcacctata taatcattta tttttccatc
     1801 tccatatata actatatttt cttttcctag aatggcattc gtatcttttt gatttacatt
     1861 tacaaccaaa gtcttaaatc caggtatatt ttccttaaga actgaagcta attctttaag
     1921 ataaggaagt ttgtttccat tcgctactaa tacaaccatt acttctttag ttgtaaaacc
     1981 tattttggtt actaaatgcc taatcaatcc tgtatgagtc ttttcatcat atatacttac
     2041 cttataagct cttatatatg tttttattat tttaacaatt ttatcattta tctcatgttg
     2101 tattacacat ttttctgtcg atattacatc atgagtcttt tttttataaa aaccaataat
     2161 tggaacacca ttattctttt gtattggaaa ttgtgcttta tttctatatc tatatggatt
     2221 atccattcct atagtgtcat gaacttctac atcttctaac ttacctattc tcgaaagtac
     2281 ttgtttaact tcatttgttt tcatttcaag ttgtttatta taatctaaat cttgtatctg
     2341 acaccctcca caatccctga gattgtcact acacactctt ttcaccctga atggagactt
     2401 ttctattatc tcaacaatat ctcctacagc ataatttttc tttactttat taatcctaac
     2461 tttaactttg tcctgtatta atccgccctc tacaaaaact gtaataccat tgtactttcc
     2521 tactcctact ccgccttgac caatgtctac tatatctaca atatacacct cgtttttcga
     2581 aagcataaat caccctccaa tcaatctatt atatttaaat ctttacaaac ttttttcttt
     2641 aaatttatat taaaatattg ctattaatta aactatattt tttactaagc tacaatattt
     2701 tattttacca aaaaaaaagg agcttataaa gccccttggt aaataaattt attatttttc
     2761 ttataaaact ctagttgaac ctctatatac cacacctctt gatgcgtcca cagttacaac
     2821 ttctccatct tttactagat ttgttatgtc agttgcagac acaataactg gtatatctaa
     2881 atttatacct acaatagctg tatgtgatgt cattccacca ttctctgtta ctacagctcc
     2941 tgctttttct atataattgt tcatttcagc atcagtagca gtagaaacaa gtatgtctcc
     3001 ttcattaaag tctacacaag tatctccatt ctttatcaca cgaactttac cttctactgt
     3061 ttgagtacct acacctatac cttgcacaat ttcttcgctt attacatgaa cctttattaa
     3121 gttagtagtt ccacttactc ctactggtac tccagcagtt ataactacta attctccatt
     3181 atctatataa tcagcttgtc tagctgcctc aattgccttt tctataactt catctgtatt
     3241 tcctgcaaca gaagacttaa ctggatatac tccccatgta agagctaatt tattcatagt
     3301 tttttcatta tttgtagtag ctattattgg agattttggt ctaaatttag ataccattct
     3361 agcagtatat cctgaagaag tagatgttat tatagctgat gcatttaaat ctactgctgt
     3421 tgtacaagtt gcatggctta ttgcatctgt tacagtaaca ttgttagtac cattttcctt
     3481 taataatcta tcataatcta aagtctcttc tgttcttttt gctattgtag ccatcatttt
     3541 tactgcttct acaggatatt ttccagcagc agtttctcca gataacatta tcgcatctgt
     3601 tccatcatat atagcattag caacgtctgt aacttctgct cttgttggtc ttggatttct
     3661 tatcatagaa tcaagcattt gagtggcagt gacaactggc tttgctaatt cattacattt
     3721 ttttatcatc attttttgaa ctattggcat ctcttcagtt ggtatctcaa cacctaagtc
     3781 acctctagct accattatac catcagaaac ttttaatatt tcatctagat tctctacacc
     3841 ttcttggttt tctattttag atattatttg tatatcagtc gcattattgt tctctaatac
     3901 ttctcttatt gctagtacat cagatgcctt tcttacaaaa gaagcagcta tataatctat
     3961 tccttgactt attccaaatt ctatatcgct tatatcttta ggagttattg ctggtaaatt
     4021 tattttaaca ccaggtacat ttactccttt atgattttta actattcctg aattttcaac
     4081 aacacaaatt atatcttctc catttatttc ttttactctt aagccaacta aaccatcatc
     4141 tattagtata gaatctcctg atttaacatc ttcagcaaga cctttatagc ttactgtaca
     4201 catttcttta gtccccataa tatctttcat agttatagtg aacttttgac cttcttctaa
     4261 aaatacctct gggtcttcaa agttacctgt tctaatttct ggaccttttg tatctaataa
     4321 tatagcaact ggcttattta atttttctct aactttcttt gccatatcta ttctttcttt
     4381 atgttcttca tgtgaaccat gagaaaagtt aaatctacac acatttagac cattttgcat
     4441 aagttgagtt aaaacttcct cactttgtga tgctggacct agtgtacaaa caatttttgt
     4501 cttctttaca ttatttaaca tatttgctcc tcctgagata ttattccaca gatttatttt
     4561 taatactaaa acttatatag ataatatttt tgccatctca taagcttctt tatcaaatac
     4621 tttcttttga gctaaagctt cacttatttc taagtcaatt attttgtttt cttttattcc
     4681 tacgactcta gctgattttc catctaataa taattctact gctcttactc caagtctact
     4741 tgctagtatt ctatcagata cagttgggct tcctcctctt tggacatgac ccaacacagt
     4801 tactcttaaa tctgctcctg actctttttt aagttccttt tctaaatcgc tggcacttcc
     4861 aactccttct gctaaaacga ttatactatg tctcttacct cttttttgag tagtctttaa
     4921 tcttaaagca acttcatcta ctgtaatttc tacttctggt actattatag tttccgctcc
     4981 tcctgcaaga cctgcatata atgctaaatc tccacagtgt cttcccataa cttctactat
     5041 attaactctt tcatgagacg atgatgtatc tcttatctta cctatagcat ctataatagt
     5101 attcattgca gtatcaaatc ctatagtata gtctgtatat gctaaatcgt tatctatagt
     5161 tcctggtata cctatagctg ggaatcctaa gtcactaagt ttttgagctc ctacgaatga
     5221 accatctcca ccaattacaa ctaagcaatc gattttgtac tttttaagaa tttttactgc
     5281 ttttaatcta ccttcttctg ttttaaattc ttcacatcta gaagatttta atattgttcc
     5341 tcccctgtgt ataatatctc caacagaaga taggttcatt tcagttaaat cttcttctag
     5401 taatccctta taccctctat tgattccata tactttgcat ccataatata ttgctgatct
     5461 tacaaccgct ctaatcgcag cattcattcc tggagcatct cctccacttg ttagtaatcc
     5521 tatagttttc attttttacc tccttggatt ttatgtcaat tttttattat aatcattgtt
     5581 attgtattct aatggacttt ttttgtccct atatcacttt ttaatcccat caagactaaa
     5641 aaaattaaat gtccttaaat gtccattttg tgatattata tcacaaaacg gacaatattt
     5701 ttcatattat tataatatat ttagattttt atataaaaaa tcaagctttt acttttatgt
     5761 tatctttagg aagtaactct tctaagttat ttataaattc atcacatata caagttaatg
     5821 cccctgtcat tttatatgct ttacgtttgc catttatatt ttcatccata gggtacaaat
     5881 atatactgtc attacccgga tattgttttg ctatttctac aatattatta gttgtatcgg
     5941 tgtcagacat actatctatt ttcaaataca attttttacc actttctggc ttaatatttt
     6001 ccacatttct agttcttcct gaattgtaat ttctatctac aaagtcagta tcatcattta
     6061 tatcttttat ctctctagct ataagttttg cattttcgcc ttcttttata cttaatgttc
     6121 cttttacata tattatatta tcttcattca aaatgacatt gtacttttgt aataattgag
     6181 gaaaaactac tatttctata gcaccataca aatcttctaa ttcaagaaat gccataattt
     6241 catttcgttt tgttgtttta atagttttag ttacaatcat tccacccatt ataacatctg
     6301 tttcattcat ttgcaggtat gactcttctt cctcttttaa tgaatttaat tttccattat
     6361 ccatagatgt tttttcttgt aattcttttt caaattgtga caaaggatgt ccacttacat
     6421 acattcccaa gacttctttt tcaaggttaa gtctctctct ctcttcgtac tcttgccttt
     6481 taggcaaact atatatttgt tgaaaatcac tatcttcctc actatctcca aaagcatcaa
     6541 ataaagaaat ttgaccttga acatttttct ttctatccat agatacactt tctaataaac
     6601 tttcataacc agccatcaaa ctagctctat tttcacttat attatcaaaa gctccacatt
     6661 ttattagact ttctattact ctcttatttg tatctttttg gtctaatctt ttaactaaat
     6721 ctactaaatc tacaaataat ccattagatt ctctttcagc aattatattg tttattatat
     6781 ttacgccaac attttttaca gctgctagac caaatcttat attgtcacct tcaactgaaa
     6841 actttgaaaa acttttgttt atatctggag gtaatacatc tatttctagt gctttacatt
     6901 ctcttatata ctcaactacc ttatcagtat ttcccattac actggttatc aatgctgcca
     6961 taaattcaac tggataatat gtctttagat aagctgtctc ataggctaaa accccatatg
     7021 cagctgcatg tgatttatta aatgcatatc gagcaaagtc tatcatgtca tcaaatattt
     7081 tattggctat ttcctcttct acaccatttc tgatacaccc tgcaatttct atattaccat
     7141 cttcatcaaa tttcccatga ataaaatatt gtctttcttc ttccatcaca tccatctttt
     7201 tcttactcat ggctcttcta actaagtcac ttcgtccgta actataacca cctaaatctc
     7261 taacaacctg cataacctgc tcttgataca ccaaacaacc atatgtaact tccattatag
     7321 gctttaattt ttcatgtagg tatgttacat cttttggatt gcttttattt tttatatatg
     7381 caggtataga atccattgga cctggtctaa atagggatat tcccgctaca atatcttcaa
     7441 aattgtctgg ttttagctgt ttcataaaac ttctcatccc agcactctcc aattgaaaca
     7501 cccctaatgt attcccagat gaaagcattt catatacttt cgggtcgtca tagtccatct
     7561 ttgaaaaatc tattttttct gtgtagtttt ttataactct atttttttct attaaatcaa
     7621 gagtatctct tataactgta agagttcgta gcccaagaaa atccatcttt aaaagcccaa
     7681 gttcttcaag tgttgtcata gtaaactgtg ttgtgattgc atcttgatgc ttgtataaag
     7741 gtacatactc atctacagga ttttttgata taacaactcc tgctgcatga gtagaagcat
     7801 gtcttaacat accctctatc tgttttgata tatttattat ttcttttgtt tctgggtctt
     7861 ggtcatacaa ttctttaaga ttaggatttg tatctaaagc cttatctatt gtcattccta
     7921 aagcaaatgg tatttccttt gctatcttgt caactttatt gtaacctata tccaatactc
     7981 tcccaacatc tcttattgca gcttttgcac ccattgttcc aaaagttatt atttgagcta
     8041 catggtcatc accatatttt ctctttacat aatctataac ttcttctctt ctctcataac
     8101 aaaaatctat atctatgtcc ggcattgata tacgttctgg atttaaaaaa cgctcaaata
     8161 atagagaata ttttattggg tctatgtcag ttatcttaag tgtatatgct actattgaac
     8221 cagccgcact acctctacct ggccctacta tgatattatt ttctttagaa aaatttataa
     8281 aatcccaaac aattaaaaaa tactctacat aacccatttt ttcaattaca tctaattcat
     8341 aatttaatct ctctagtatc tcatcactag gattatcata cctctctttt aatccattaa
     8401 aacacaactc tcttaaatag gtttctggtg tatacccttc tggaacatca tacttaggaa
     8461 gatgtatagt gttaaaatca aactccacat tacacatatc agctattttc acagtattgt
     8521 caatagcctc taatgcatat ggaaataatt cttccatttc ttctctcgat tttaaataaa
     8581 actcatcact accaaatctc attctatttg ggtcatttaa tgtttttccc atttgaatac
     8641 acataagaac atcatgtatt ttcgaatctt ctttatttac atagtgaaca tcattagtag
     8701 caactagtgg tataccagtc tcttttgata gttttactaa tccagagttt acttcttttt
     8761 gctcaggtaa attgtgatct tgaatctcca aaaagaaatt ttcttctcca aatatatctc
     8821 tgtagtcaaa agcaaattgt tttgcttttt cataatttct attcattaaa gcacgtgcta
     8881 catctcctgc aaggcatgca gaaagtgcta taatcccttt gttatgtttt ttaagttctt
     8941 ccatgtcaac tctaggctta tagtaaaaac cttctacgta tgaagttgaa actattttta
     9001 ttaaattttt atatccttcc atattcttag ctaacagaac taaatgtcct tgatatttat
     9061 catagtttgg gtctttatct ctaagtccac gtgcagctgt atagacttcg caccctataa
     9121 tcggctttat tccttctttt atagccgttt tgtagaaatc tataacacca aacatacaac
     9181 catggtcagt tatagctatt gagctcatgt ttagttcctt agcccttttt ataagttttt
     9241 tcaccctcga aaacccatca agtaaactat actcagtatg tacatgaaga tgagcaaaat
     9301 ctttgttcat taaatcacct cctgctttat aattttacca caaatatatg tcctaaaaca
     9361 aaaacagaga cttttatctc aaaataggaa ttaacttcaa catataccat tatatgttat
     9421 aaattaatgc ttattctgag attgtcccct caatttattg tgatgatttt tttttcaaat
     9481 ctgtcgcaat ttcttctatt tttctaagcc tatgattgac tccagatttt cctattggtg
     9541 gttctaacat ctctccaagt tctttgagag tcatatcctc atactcgatt ctaatcttag
     9601 ctatttcttg taaattttct ggcaaactgc taattcctat agtttcttgt atgagcttaa
     9661 tattttctac ttgtcttact gctgcattta ctgttttaga aaggttagct gtctcacaat
     9721 ttactatcct gtttacatta tttctcattt cttttactat tttagtattt tgaagactta
     9781 aaagagcatg gtgtcctcct ataatactca acaaatctga tatttgctca ctctctttga
     9841 tataaacaac gtgattattt tttctagcca caattttact attaaatcct aaagagttta
     9901 taagttcttt tagagaatct gcaaactcct cattattagt tacaaattcc atatgatagt
     9961 tcttttctgg gtcgcttata gaacctccac caagaaaagc tcctcttatg aaagctctct
    10021 tacatccttc atgcttaatt aagttatctg aaattttgtt tatagtaaaa aatccttctt
    10081 tattttcaag tatacctaac ttttttaaca acatttctga acccatttca cttgttacca
    10141 ttaatacata actgttattt ctctttagca tttgattctt atttaccgag attgtagtat
    10201 tgatattaaa attcttcttt aataatttaa atactttacg ggctattgaa tttaactctg
    10261 ttgttatctt tagatttaac ttcttatatc caacaatttg tatacttccc cctgatttaa
    10321 ctagagctga caattcagct atattacaac attcattccc agatacaact ctcgctagtt
    10381 catttttggt ttcagttgaa aaagacatat ctatcaccta ttaactaatt tttctttaac
    10441 ctttttttta tttcttgtaa ggcaaccttt attatctcct tttcatcatc atacctagct
    10501 aggtctacta ctctatctag atggataaat ttagcatcaa taaagccttc ctctttttgt
    10561 ggaatagtat ccatattctt tgcttgcatc aaataccaaa aaactgtttt atgaactgct
    10621 ctattttcat cccagttttc tttgaatgta tagtgtattt cacctaaata tttcaatata
    10681 tctgctttta ctccagtttc ttcactcact tcacgtaatg ctgcctcttg gttattttcc
    10741 ccttcttcta cttttccctt gggtaacacc caatctccat tgaacttcct tagtaaaaga
    10801 attgtattgc caaacaggac tacaccacca gcactaacct cttctctcat cgatgttcac
    10861 tccttaaaac taacataatg ataaggattt ctacacaatt ttttaaaatc cttttttact
    10921 aacttttgtt gtaattatga tttagggcta actcaataac tatgttagat atatactttg
    10981 catcatgtct tatataatta ttttttattt caattaaatt cttctctaca gtttttatac
    11041 ccatctcttt taatttatct ttttgctttt tatctaacaa aacttgttct gctccatctt
    11101 gtttgtataa gtcaagcatt tcttctggca gtatctcatt atttgcgatt acataatcta
    11161 ttaaattctt atcagtatgc tttactatag cattaacatg ctctaataca ttgtaccctt
    11221 ctgtctcacc gggttgagtc attatatttg ctatatatac ttttggagct acagacgatt
    11281 taatagcgtc tacaatcccc tctacaagta aatttggtat tatactcgta tataagcttc
    11341 cagggcccat aattataatg tcagcatcgt aaatagatgc aataacatcc tttaatggtt
    11401 tcacatcttt tggatttaaa aaaattttat caatagagct tttttgaatt tttgactctt
    11461 caggtatact agattctcca tttattatat tgccattttc taatttggca actaaattta
    11521 catcttccaa ggttactggc aaaactcttc ctgttatagc aaaaatctca cttaatttat
    11581 atacagcttt ttcaaagttt ccatacaacc cattcatagc agccaaaaac aaatttccaa
    11641 aactctgacc ttttaaaagc ccttctgtaa acctatattg cataacttca ttcattgttg
    11701 gctctatatt tgcaagtgct aatagacagt ttcttatgtc tcctggtggg agcattccta
    11761 agtcttctct caatacacct gaacctccac catcatcagc aacagttaca attgcagtta
    11821 tattttgcgt tgtgtgttta aggcctctta aaaatactga ttgcccagta cctccaccta
    11881 ttacaaccac atttggttct ctattttcaa caatagacct ttgaaataat tttacttttt
    11941 cattttttgc ttttttatat ataaataacg atataattaa tgcaatccaa cctaaaattc
    12001 ctacaataat aagtaattta gccatgtttg cctccattat cgcaacatag agtctctatg
    12061 tttctttaca actctatatc ccttattaga taaatcctcc gctataagat ttgttatagt
    12121 aactgaccta tgtcttccac ctgtacatcc aacaccaatt actagatgtt gttttccttc
    12181 ctctatatat tgaggtacta aaaaatgaat catatctaaa agtttcacat aaaattcttc
    12241 actgatttta gaattcataa cataatctct aacttcttta tcatcaccag ttttagctct
    12301 taactcttca acataatagg gatttggaag aaatcttaca tcaaatacta aatctgcatc
    12361 agcaagtata ccatgtttaa atccaaaaga tactacagaa atagttaggt ttggattatc
    12421 ttcaccagaa gaatatattt tctttatttc ttccttaagg tctttgggct tcatgttagt
    12481 tgtatcaatt atacatgtag ataattcctt taaaggttcc attatctttc tttccatttt
    12541 tataccttct ggaatctgca tatcttttgc caatggatga tttcttcttg tcattttata
    12601 tctctttaac aatacatcat cattacaatc caaatatacc atttcatatt cataattttc
    12661 tttttctaaa taatttaagc tttcatgaag cgcttcaaaa aactttctcc ctcttatatc
    12721 tatccctaga gcaaccttat caatacttga atttggttga taacataatt ctgcaaattt
    12781 agttatcaaa gcaggaggta gattatctac acaataaaat cccatatcct ctaaagctct
    12841 cattgtctca ctttttccag atcctgaaag acctgtaact ataacaaatt tcattgctaa
    12901 ctcctccctt ttatcttatg tttattatat tataatgttt tctactaacg atatatccag
    12961 attagagtca gatatagtct ttatagctag ttcaaaatcg ccaactcttt ctggacaatg
    13021 tgcatctgag cctattataa atttattttc tagccctttt atctcattta attgatttaa
    13081 gttcaaaaaa gaatggctac tgtttatttc aagtctagtg tcagtctttt ttgctgtctt
    13141 tgctatttcc tctatataca catcgccttt atctcctgga tgagtaatta taaaaatatc
    13201 attattactt atcgcatttg ctacagcttt tgtattatat tcttttgctt tttcagtttt
    13261 aaaaatatag ttattacaat gatttaataa acttcttaat gatgttggag tagaaccaaa
    13321 atgatatcca gccataatat agtcaaaata tctaatgaac ttatcatcca tatctatatt
    13381 gcctttatcg tctaatatgt tacactccat acctaaaagt atatttatat cattgtactt
    13441 atcatttaaa taatctactt cttctctcat tttaagaata tcttttttct ttacaccata
    13501 tgcaatatgt ttataactat ggtcagatat tcctattgtt tttattcctt tagaaatggc
    13561 cacctttaca ttatcctcaa tagtcccttt gccatgacta tataatgtat gtgtatgata
    13621 atcagccagt attttcattt gttaatcctc tccaagtatt ttgacttctt cttccaacat
    13681 aaccccaaac ttggcatata cagcactttt tataacatat attaaatcca aaatatcttt
    13741 agcacttgct tctccttgat ttactacaaa tccacaatgc ttctcagaca cttgtgctcc
    13801 tcttaaagta agacctctca atccagtttc ttctattaat ttacctgcaa aatatcctgt
    13861 tggtctctta aatgtacttc ctgcactagc aaaatttaat ggctgtttag ttattcttct
    13921 cgtcgctaaa tcatccatta aactttttat ttcatcataa ttgccttctt gtaattctaa
    13981 ttctgctgat aaagctatat atccattttt cgatattata ctttttctat acccaaattc
    14041 catctgttta tttgacaatt caaatatatt gccttccata tccatcactc ttactgactt
    14101 tactatgtcc ttcatttcac caccatatgc tccagcattc atggctaatg ctcctcccaa
    14161 tgtcccaggt attcctgatg caaactcaaa cccttttaat tcttgctttt ggagagcttt
    14221 acctaaaact gataataaag ctcctgactg agcagtcatc ctagtatcat ttatctcata
    14281 agagttaaaa ttatcagcta attctattac cactcctctt attccaccat ctttaattaa
    14341 aatgtttgaa ccatttccaa ttattaaata cggaatagat tcttttttta ccaacttaag
    14401 tacatttttt aattgttctt ctgttctagg tctaactaat atatctgctg gtcctcctac
    14461 cctaaatgaa atatgttttt tcataggttc atcgactttt atatcctctt tacttaatat
    14521 atttaataaa tttatatata tatcttgatt attcattgta tttcctccca aattatactg
    14581 aactttagct gtcattatta tttaaattat attagtatat agtattaact atattcaata
    14641 atatatgatt ttgcttatca tatggatttt atattttaag cttcttaatt acaaactata
    14701 ttcaataaat atataataaa gtaaaaaaaa aaaaataaca acatataaaa gaatataaat
    14761 aatttaaaaa aagcatcaat atcactgata tcaatgcttt aaaaggcaat ttaattcata
    14821 ttatttatct aactattttt gacatagcaa agataataaa tccctccaac taccatggaa
    14881 cctccaacga tatttccaag cgttacaaat accaaattag ttatgaattg aaccatagtt
    14941 atatttgctc ctagtgccat tcccattggt ataaaaaaca tgtttgcaac actatgttgg
    15001 aatccacata atacaaatag cattattact gtccaacatg aagccaattt tgacattata
    15061 tcttgagatg ctgtagacat ccatacagct aaaacgacca gaatattaca aaggattgct
    15121 tttaaaaaca ttgatgctat agttgaatta accttagctt ctgccacagc tatagctttt
    15181 gtagctgcat ctcctgtcaa tgtagaagaa taaaacacaa gtgcgactaa caaaattgaa
    15241 cctactaaat ttgcaaaata aaccagtccc cagtttctta acatttttcc taatgttatt
    15301 tttttgtcca taagtgcaat agccattaaa gtgtttccag taaacaactc tgctcctgct
    15361 actactacaa gcataagacc tactggaaag accatagcac ttaaaaagct agctacccct
    15421 gggtctatct tgccaaaagt ttgactaact atcatactcc ccaatgcccc aaaacctata
    15481 aatgctcctc caaataaacc taataaaatt gcattttgtg tagaaatgtt tgctttttta
    15541 atgcctgcac ttatagttgc ttgtgcagtt tcaccaggtg tcaagaatcc tttactcata
    15601 aatccttccc ccttaaaaat tatactttcc cttgtctttt tatggcaata attatataat
    15661 aacaatatga tatataataa attttattaa cataaaaaat atactatatg cttatatata
    15721 cttgtcttat ataattatac tatttattat ataacaactt tgttaatttc gaaataattt
    15781 atattgaatt tgaaaaattt accttttgat tatttatttt acttttt
//
LOCUS       DAMJVM010000079        15805 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.082, whole genome shotgun sequence.
ACCESSION   DAMJVM010000079 DAMJVM010000000
VERSION     DAMJVM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.082"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            132..686
                     /gene="tcdR"
                     /locus_tag="PC523_002661"
     CDS             132..686
                     /gene="tcdR"
                     /locus_tag="PC523_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HDF2476168.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLNYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKTKFNFVEFDE
                     NILNMKNNYQSKSVFEEDICFFEYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            1004..8104
                     /gene="tcdB"
                     /locus_tag="PC523_002662"
                     /gene_synonym="toxB"
     CDS             1004..8104
                     /gene="tcdB"
                     /locus_tag="PC523_002662"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HDF2476169.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTIVESAINDTLESFREN
                     LNNPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKIYLSNEYSKEI
                     DELNTYIEESLNKIKQNSGNDVRNFEEFKNGESFKLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPGYTSEH
                     FDMLDEEVQSSFESALASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTVNLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWTFDDARAKVQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSILFQKNIENSEVAYYYNPG
                     DGEIQEIDKYRIPSIISDRPKIKLTFIGHGKDEFNTDIFAGLDVDSLSTEIETAIDLA
                     KEDISSKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINNEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNLSDIELEEKVMLAECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     NEFKLIESISDSLYDLKQQNELDDSHFISFEDISKTEDGFSIRFINKETGESIFVETE
                     KEIFSEYANHIEREISNIKDTIFDTVNGKLVKKVNLDAIHEVNTLNAAFFIQSLIGYS
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     VIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTAATTAIITSSLGIASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHISLVETEGAFTLLDDKIMIP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTNDIDHFFSSPTITYIKPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENEGTKLLDRIRDHYKGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNNRSFIVPIITTEHIREKLSYSFHGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIDSDKIKKGDLIEGILSTLSIE
                     DNKIILNHHEINFSGDVNGSNGFISLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSFTLQDEKTIKL
                     NGVHLDESGVAEILKFMNKKGSTNTSDSLMSFLESVNIKSIFVNFLQSKINFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETTYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNNYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIISAFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPTNGGAASIGETIINDKNYYFNQSGILQTGVFS
                     TEDGLKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFEDNYRGAVEWKELDGEMYYF
                     SPETGKAFKGLNQIGDDKYYFNSDGIMQKGFVSINDKKYYFDDSGVMKVGYIEIDGKY
                     FYFAENGEMQIGVFNTSDGFKYFAHHNEDLGNEEGEAISYSGILNFNNKIYYFDYSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYVGLSLINDGQYYFNDDGIMQVGFVTINNKVFYFS
                     DSGIIESGVQNIDDNYFYIDEKGIVQIGVFDTSDEYKYFAPANTVNDNIYGQAVDYSG
                     LVRVGEDIYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEN
                     GIMRTGLISFENNDYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTQDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            8226..8726
                     /gene="tcdE"
                     /locus_tag="PC523_002663"
     CDS             8226..8726
                     /gene="tcdE"
                     /locus_tag="PC523_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HDF2476170.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAVLLDAMTDEM
                     NAKDEK"
     gene            8805..8966
                     /locus_tag="PC523_002664"
     CDS             8805..8966
                     /locus_tag="PC523_002664"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476171.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYMDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            9055..9237
                     /locus_tag="PC523_002665"
     CDS             9055..9237
                     /locus_tag="PC523_002665"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476172.1"
                     /translation="MRDEGQNDRENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            9454..>15805
                     /gene="tcdA"
                     /locus_tag="PC523_002666"
                     /gene_synonym="toxA"
     CDS             9454..>15805
                     /gene="tcdA"
                     /locus_tag="PC523_002666"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021394011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HDF2476173.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKNSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFVNYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     LLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNEVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKDSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDTSVSPDTKFILNNLKLNIESSVGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDLLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKEYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNAIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIKTENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENNGTKLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNGSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQAKV
                     NGLYLNESVYSSYLDFVKNADGHHNTSNFMNLFLDNISFWKLFGFENINFVVDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLVYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEASKLVKGLININNSLFY
                     FDPIESNLVTGWQTINGKKYYFDINTGAALTSYKIINGKHFYFNNNGVMQLGVFKGPD
                     RFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSGSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAE"
BASE COUNT         6391 a         1652 c         2469 g         5293 t
ORIGIN      
        1 tgtttataat gaatattttc atataaaact taatttattt tctaatcata caattataat
       61 gactgattta atgcatatgt tgtcaatttt ttttaaaaaa tcatcattat aaaataagag
      121 aggatgattt tatgcaaaag tctttttacg aattaattgt tttagcaaga aataactcag
      181 tagatgattt gcaagaaatt ttatttatgt ttaagccatt agtaaaaaaa cttagtaggg
      241 ttttaaatta tgaagaggga gaaacagatt taataatatt ttttattgaa ttaataaaaa
      301 atattaaatt aagtagcttt tcagaaaaaa gtgatgctat tatagttaaa tatattcata
      361 aatcattatt gaataagact tttgagttat ctagaagata ttctaagact aagtttaatt
      421 ttgtagaatt tgacgaaaat atcttaaata tgaaaaataa ttatcaaagt aagtctgttt
      481 ttgaggaaga tatttgtttt ttcgaatata ttttgaaaga attatctggt attcaaagaa
      541 aagttatttt ttataaatat ttaaaaggat attctgatag agaaatatca gtgaaattaa
      601 aaatatctag acaagctgtt aataaggcta aaaatagagc atttaaaaaa ataaaaaaag
      661 actatgaaaa ttattttaac ttgtaattaa tgagtttaaa gaaatattta caatagaaat
      721 caaattttag aattaacttt attgtaaaat caataactta atctaagaat atcttaattt
      781 ttatatttta tatagaacaa agtttacata tttatttcag acaacgtctt tattcaattg
      841 aagagcaaat taatcaactg agtgtcttcg atttaaaatg ttaggaagtg aatgtatatg
      901 aaaacctaag tagatattag tatattttat aaatagaaag gaggatatat aaaagagttt
      961 tagcatttag atgtaaaaat attatagtaa aggagaaaat tttatgagtt tagttaatag
     1021 aaaacagtta gaaaaaatgg caaatgtaag atttcgtact caagaagatg aatacgttgc
     1081 aatattggat gctttagaag aatatcataa tatgtcagag aatactgtag tcgaaaaata
     1141 tttaaaatta aaagatataa atagtttaac agatatttat atagatacat ataaaaaatc
     1201 tggtagaaat aaagccttaa aaaaatttaa ggaatatcta gttacagaag tattagagct
     1261 aaagaataat aatttaactc cagttgagaa aaatttacat tttgtttgga ttggaggtca
     1321 aataaatgac actgctatta attatataaa tcaatggaaa gatgtaaata gtgattataa
     1381 tgttaatgtt ttttatgata gtaatgcatt tttgataaac acattgaaaa aaactatagt
     1441 agaatcagca ataaatgata cacttgaatc atttagagaa aacttaaata accctagatt
     1501 tgactataat aaatttttca gaaaacgtat ggaaataatc tatgataaac agaaaaattt
     1561 cataaactac tataaagctc aaagagaaga aaatcctgaa cttataattg atgatattgt
     1621 aaagatatat ctttcaaatg agtattcaaa agagatagat gaacttaata cctatattga
     1681 agaatcctta aataaaatta aacagaatag tggaaatgat gttagaaact ttgaagaatt
     1741 taaaaatgga gagtcattca aattatatga acaagagttg gtagaaagat ggaatttagc
     1801 tgctgcttct gacatattaa gaatatctgc attaaaagaa attggtggta tgtatttaga
     1861 tgttgatatg ttaccaggaa tacaaccaga cttatttgag tctatagaga aacctagttc
     1921 agtaacagtg gatttttggg aaatgacaaa gttagaagct ataatgaaat acaaagaata
     1981 tataccagga tatacttcag aacattttga catgttagat gaagaagttc aaagtagttt
     2041 tgaatctgct ctagcttcta agtcagataa atcagaaata ttctcatcac ttggtgatat
     2101 ggaggcatca ccactagaag ttaaaattgc atttaatagt aagggtatta taaatcaagg
     2161 gctaatttct gtgaaagact catattgtag caatttaata gtaaaacaaa tcgagaacag
     2221 gtataaaata ttgaataata gtttaaatcc agctattagc gaggataatg attttaatac
     2281 tacaacgaat acctttattg atagtataat ggctgaagct aatgcagata atggtagatt
     2341 tatgatggaa ctaggaaagt atttaagagt tggtttcttc ccagatgtta aaactactgt
     2401 taacttaagt ggccctgaag catatgcggc agcttatcaa gatttattaa tgtttaaaga
     2461 aggcagtatg aatatccatt tgatagaagc tgatttaaga aactttgaaa tctctaaaac
     2521 taatatttct caatcaactg aacaagaaat ggctagctta tggacatttg acgatgcaag
     2581 agctaaagtt caatttgaag aatataaaag gaattatttt gaaggttctc ttggtgaaga
     2641 tgataatctt gatttttctc aaaatatagt agttgacaag gagtatcttt tagaaaaaat
     2701 atcttcatta gcaagaagtt cagagagagg gtatatacac tatattgttc agttacaagg
     2761 agataaaatt agttatgaag cagcatgtaa cttatttgca aagactcctt atgatagtat
     2821 actgtttcag aaaaatatag aaaattcaga agttgcatat tattataatc ctggagatgg
     2881 tgaaatacaa gaaatagaca agtatagaat tccaagtata atttctgata gacctaagat
     2941 taaattaaca tttattggtc atggtaaaga tgaatttaat actgatatat ttgcaggtct
     3001 tgatgtagat tcattatcta cagaaataga aacagcaata gatttagcca aagaggatat
     3061 ttcttctaag tcaatagaaa taaatttatt aggatgtaat atgtttagct actctatcaa
     3121 cgtagaagag acttatcctg gaaagttatt acttaaagtt aaagataaaa tatcagaatt
     3181 aatgccatct ataagtcaag actctattat agtaagtgca aatcaatatg aagttagaat
     3241 aaataatgaa ggaagaagag aattattgga tcattctggt gaatggataa ataaagaaga
     3301 aagtattata aaggatattt catcaaaaga atatatatca tttaatccta aagaaaataa
     3361 aattacagta aaatctaaaa atttacctga gctgtctaca ttattacaag aaattagaaa
     3421 taattctaat ttaagtgata ttgaactaga agaaaaagta atgttagcag aatgtgaaat
     3481 aaatgttatt tcaaatatag atacacaaat tgttgaggaa aggattgaag aagctaagaa
     3541 tttaacttct gactctatta attatataaa aaatgaattt aaactaatag aatctatttc
     3601 tgattcctta tacgatttaa aacaacaaaa tgaattggat gattctcatt ttatatcttt
     3661 tgaggatata tcaaagacag aagatggatt tagtataaga tttattaata aagaaactgg
     3721 agaatctata tttgtagaaa ctgaaaaaga aattttttct gaatatgcta atcatataga
     3781 gagagaaatt tctaatataa aagatactat atttgatact gtaaatggta agttagtaaa
     3841 aaaagtgaat ttagatgcta tacatgaagt aaatacttta aatgctgcat tttttataca
     3901 atcattaata ggatatagta gttctaaaga atctcttagt aatttaagtg tagcaatgaa
     3961 agttcaagtt tatgctcaat tatttagcac tggtttaaat actattacag atgcagccaa
     4021 agttgttgaa ttagtatcaa ctgcattaga tgaaactata gacttacttc ctacattatc
     4081 tgaaggatta cctgtaattg caactatcat agatggtgta agtttaggtg cagcaatcaa
     4141 agagttaagt gaaactagtg acccattatt aagacaagaa atagaagcta agataggtat
     4201 aatggcagta aacttaacag cagctacaac tgcaatcatt acttcatctt tagggatagc
     4261 tagtggattt agtatacttt tagttccttt agcaggtatt tcagcaggta taccaagctt
     4321 agtaaataat gaacttgtac ttagagataa ggcaacaaag gttgtagatt attttaaaca
     4381 tatttcatta gttgaaactg aaggagcatt tactttatta gatgataaaa taatgatacc
     4441 acaagacgat ttagtgatat cagaaataga ttttaataat aattcaatag ttttaggtaa
     4501 atgtgaaatc tggcgaatgg aaggtggctc aggtcataca gtaacaaatg atatagatca
     4561 cttcttttca tcaccaacaa taacatatat aaaaccacac ttatctatct atgatgtatt
     4621 ggaagtacaa aaagaagaac ttgatttgtc aaaagattta atggtattac ctaatgctcc
     4681 aaatagagta tttgcttggg aaacaggatg gacaccaggt ttaagaagtt tagaaaatga
     4741 gggtacaaaa ctgttagacc gtataagaga tcactacaaa ggtgagtttt attggagata
     4801 tttcgctttt atagctgatg ctttaataac aacattaaaa ccacgatatg aagatactaa
     4861 tataaggata aatttagata gtaacaatag aagttttata gttccaataa taactacaga
     4921 acatataaga gaaaaattat catattcttt ccatggctca ggaggaacat atgcattatc
     4981 tctttctcaa tataatatgg gtataaatat agaattaagt gaaagtgatg tttggattat
     5041 agatgttgat aatgttgtga gagatgtaac tatagactct gataaaatta aaaaaggtga
     5101 tttaatagaa ggtattctat ctacactaag tattgaagat aataaaatca tcttaaatca
     5161 tcatgagatt aatttttctg gagatgtaaa tggaagtaat ggatttattt ctttaacatt
     5221 ttcaatttta gaaggaataa atgcaattat agaagttgat ttattatcta aatcatataa
     5281 attacttatt tctggcgaat taaaaatatt gatgttaaat tcaaatcata ttcaacagaa
     5341 aatagattat ataggattca atagcgaatt acagaaaaat ataccatata gctttgtaga
     5401 tagtgaagga aaagagaatg gttttattaa tggttcaaca aaagaaggtt tatttgtatc
     5461 tgaattacct gatgtagttc ttataagtaa ggtttatatg gatgatagta agccttcatt
     5521 tggatattat agtaataatt tgaaagatgt caaagttata actaaagata atgttaatat
     5581 attaacaggt tattatctta aggatgatat aaaaatctct ctttctttta ctctacaaga
     5641 tgaaaaaact ataaaattaa atggtgtgca tttagatgaa agtggagtag ctgagatttt
     5701 gaagttcatg aataaaaaag gtagtacaaa tacttcagat tctttaatga gttttttaga
     5761 aagtgtgaat ataaaaagta ttttcgttaa tttcttacaa tctaaaatta actttatatt
     5821 agatgctaat tttataataa gtggtactac ttctattggt caatttgagt ttatttgtga
     5881 tgaaaatgat aatatacaac catatttcat taagtttaat acactagaaa ctacttatac
     5941 tttatatgta ggaaatagac aaaatatgat agtagaacca aattatgatt tagatgattc
     6001 tggagatata tcttcaactg ttatcaattt ctctcaaaag tatctttatg gaatagacag
     6061 ttgtgttaat aaagttgtaa tttcaccaaa tatttataca gatgaaataa atataacgcc
     6121 tgtatatgaa acaaataata attatccaga agttattgta ttagatgcaa attatataaa
     6181 cgaaaaaata aatgttaata tcaatgatct atctatacga tatgtatgga gtaatgatgg
     6241 taatgatttt attcttatgt caactagtga agaaaataag gtgtcacaag ttaaaataag
     6301 attcgttaat gtttttaaag ataagacttt ggcaaataag ctatctttca actttagtga
     6361 taagcaagat gtgcctgtga gtgaaataat ctcagcattt acaccttcat attatgagga
     6421 tggattgatt ggctatgatt tgggtctagt ttctttatat aatgaaaaat tttatattaa
     6481 taactttgga atgatggtat ctggattaat atatattaat gattcattat attactttaa
     6541 accaccagta aataatttga taactggatt tgttactgta ggtgatgata agtattactt
     6601 taatccaact aatggtggag ctgcctcaat tggagagaca ataattaatg acaaaaatta
     6661 ttatttcaac caaagtggaa tcttacaaac aggtgtattt agtacagaag atggacttaa
     6721 atattttgcc ccagctaata cacttgatga aaacctagaa ggagaagcaa ttgattttac
     6781 tggaaaatta attattgacg aaaatattta ttattttgaa gataattata gaggagctgt
     6841 agaatggaaa gaattagatg gtgaaatgta ctattttagc ccagaaacag gcaaagcttt
     6901 taaaggtcta aatcaaatag gtgatgataa atactatttt aattctgatg gaattatgca
     6961 aaaaggattt gttagtataa atgataagaa atattatttt gatgattctg gtgttatgaa
     7021 agtgggttat attgaaatag atggcaagta tttctacttt gctgaaaatg gagaaatgca
     7081 aataggagta tttaatacat cagatggatt taaatatttt gctcatcata atgaagacct
     7141 aggaaatgaa gaaggtgaag caatttcata ttctggtata ttaaatttca ataataaaat
     7201 ttactatttt gattattcat ttacagctgt agttggatgg aaagatttag aggatggttc
     7261 aaagtattat tttgatgaag atacagcaga agcatatgta ggtttatcat taatcaatga
     7321 tggtcaatat tattttaatg atgatggaat tatgcaagtt ggatttgtca ctataaataa
     7381 taaagttttc tacttctctg attctggaat tatagaatct ggagtacaaa atatagatga
     7441 taattatttc tatatagatg agaagggtat agttcaaatt ggcgtatttg atacttcaga
     7501 tgaatataaa tactttgcac ctgctaatac tgtaaatgat aatatttacg gacaagcagt
     7561 tgactatagc ggtttagtta gagttggtga agatatatat tattttggag aaacctatac
     7621 aattgagact ggatggatat atgatatgga aaatgaaagt gataaatatt atttcaatcc
     7681 agaaactaaa aaagcatgca aaggtattaa tttaattgat gatataaaat attattttga
     7741 tgagaatggc ataatgagaa cgggtcttat atcatttgaa aataatgatt attactttaa
     7801 cgagaatggt gaaatgcaat ttggttatat aaatatagaa gataagatgt tttattttgg
     7861 tgaagatggt gtcatgcaga ttggagtatt taatacgcaa gatggattta aatactttgc
     7921 acatcaaaat actttggatg agaattttga gggagaatca ataaactata ctggttggtt
     7981 agatttagat gaaaagagat attattttac agatgaatat attgcagcaa ctggttcagt
     8041 tattattgat ggtgaggagt attattttga tcctgataca gctcaattag tgattagtga
     8101 atagataaaa atatgttaaa tatatcctct tatacttaaa tatataaaaa taaacaaaat
     8161 gatacactac ataaagtgtt ctatctaata tgaagattta ccaataaaaa ggtgaactat
     8221 gatgaattca cagtagttca cctttttata tttctaatgg taacaaaata tttttttata
     8281 taaacctagg aggcgttatg aatatgacaa tatctttttt atcagagcat atatttataa
     8341 agttagtaat tttaactata tcatttgata cattattagg atgtttaagt gcaataaaaa
     8401 gtcgtaaatt taattctagt tttggaatag atggaggaat cagaaaagta gcaatgatag
     8461 catgtatatt ttttttatca gtagttgaca ttcttacaaa gtttaacttt ttatttatgt
     8521 taccacaaga ttgtatcaat tttttaagac taaaacatct tggaatatct gaatttttct
     8581 ctattttatt tattttatat gaaagtgtaa gtatattaaa aaatatgtgc ttatgtggat
     8641 taccagtacc taagagatta aaggaaaaaa tagcagtttt actagatgca atgacagatg
     8701 aaatgaatgc taaggatgaa aagtaagtac tggtagatat aataaagata ttaacaaata
     8761 aaaagtgtta tccaaataag aatagctgaa agttatcata attcatgaaa ctgataatga
     8821 aaacgaggga gcagatgcca agagacacac aagtattaaa tacatataat ttcgaagcaa
     8881 gtgttcatta ctatatggat gacaaggtag tatatcaaac attggttcac aaagatggtg
     8941 catggtcagt tggtaaaatc tattaagcta cattagttac agatatcaca aactataata
     9001 gttaaacata gaaatatgtg taaattgtga tggaaattat tcaaaaacac aaaaatacgt
     9061 gatgaaggac aaaatgatag agaaaataag tatcaaacct taataaatga tttaattgat
     9121 agtttaaaag ttataggaaa aatatataaa gaaataaaaa cattaaaaaa atataagata
     9181 tgtttacaaa ttactatcag acaatctcct tatctaatag aagagtcaat taactaattg
     9241 agtatcttta aattgaaatg ttaggaagtg atttaaatat gaaaacttaa attataaaaa
     9301 atcaatatta atttattttt aaaaaataga aaggagtgta taagatttat tttcaaagtt
     9361 taaaaaaaag aaaatcaatt taaatttcag aaggaataaa tgtggttata gaagtggatt
     9421 tattatcaaa aataataata ctaggaggtt tttatgtctt taatatctaa agaagagtta
     9481 ataaaactcg catatagcat tagaccaaga gaaaatgagt ataaaactat actaactaat
     9541 ttagacgaat ataataagtt aactacaaac aataatgaaa ataaatattt acaattaaaa
     9601 aaactaaatg aatcaattga tgtttttatg aataaatata aaaattcaag cagaaataga
     9661 gcactctcta atctaaaaaa agatatatta aaagaagtaa ttcttattaa aaattccaat
     9721 acaagccctg tagaaaaaaa tttacatttt gtatggatag gtggagaagt cagtgatatt
     9781 gctcttgaat acataaaaca atgggctgat attaatgcag aatataatat taaactgtgg
     9841 tatgatagtg aagcattctt agtcaataca ctaaaaaagg ctatagttga atcttctacc
     9901 actgaagcat tacagctact agaggaagag attcaaaatc ctcaatttga taatatgaaa
     9961 ttttacaaaa aaaggatgga atttatatat gatagacaaa aaaggtttgt aaattattat
    10021 aaatctcaaa tcaataaacc tacagtacct acaatagatg atattataaa gtctcatcta
    10081 gtatctgaat ataatagaga tgaaacttta ttagaatcat atagaacaaa ttctttgaga
    10141 aaaataaata gtaatcatgg gatagatatc agagctaata gtttgtttac agaacaagag
    10201 ttactaaata tttatagtca ggagttgtta aatcgtggaa atttagctgc agcatctgac
    10261 atagtaagat tattagccct aaaaaatttt ggcggagtat atttagatgt tgatatgctt
    10321 ccaggtattc actctgattt atttaaaaca atatctagac ctagctctat tggactagac
    10381 cgttgggaaa tgataaaatt agaggctatt atgaagtata aaaaatatat aaataattat
    10441 acatcagaaa actttgataa acttgatcaa caattaaaag ataattttaa actcattata
    10501 gaaagtaaaa gtgaaaaatc tgagatattt tctaaattag aaaatttaaa tgtatctgat
    10561 cttgaaatta aaatagcttt cgctttaggc agtgttataa atcaagcctt gatatcaaaa
    10621 caaggttcat atcttactaa cctagtaata gaacaagtaa aaaatagata tcaattttta
    10681 aaccaacacc ttaacccagc catagagtct gataataact tcacagatac tactaaaatt
    10741 tttcacgatt cattatttaa ttcagctacc gcagaaaact ctatgttttt aacaaaaata
    10801 gcaccatact tacaagtagg ttttatgcca gaagctcgct ccacaataag tttaagtggt
    10861 ccaggagctt atgcatcagc ttactatgat ttcataaatt tacaagaaaa tactatagaa
    10921 aaaactttaa aagcatcaga tttaatagaa tttaaattcc cagaaaataa tctatctcaa
    10981 ttgacagaac aagaaataaa tagtctatgg agctttgatc aagcaagtgc aaaatatcaa
    11041 tttgagaaat atgtaagaga ttatactggt ggatctcttt ctgaagacaa tgaggtagac
    11101 tttaataaaa atactgccct cgataaaaac tatttattaa ataataaaat tccatcaaac
    11161 aatgtagaag aagctggaag taaaaattat gttcattata taatacagtt acaaggagat
    11221 gatataagtt atgaagcaac atgcaaccta ttttctaaaa atcctaaaaa tagcattatt
    11281 atacaacgaa atatgaatga aagtgcaaaa agttactttt taagtgatga tggagaatct
    11341 attttagaat taaataaata taggatacct gaaagattaa aaaataagga aaaagtaaaa
    11401 gtgaccttta ttggacatgg taaagatgaa ttcaacacaa gcgaatttgc tagattaagt
    11461 gtagattcac tttccaatga gataagttca tttttagata ccataaaatt agatatatca
    11521 cctaaaaatg tagaagtaaa cttgcttgga tgtaatatgt ttagttatga ttttaatgtt
    11581 gaagaaactt atcctggtaa gttactatta agtattatgg acaaaattac ttccacttta
    11641 cctgatgtaa ataaagattc tattactata ggagcaaatc aatatgaagt aagaattaat
    11701 agtgagggaa gaaaagagct tctagctcac tcaggtaaat ggataaataa agaggaagct
    11761 attatgagcg atttatctag taaagaatac attttttttg actctataga taataagcta
    11821 aaagcaaagt ccaagaatat tccaggatta gcgtcaatat cagaagatat aaaaacattg
    11881 ttacttgata caagtgttag tcctgataca aaatttattt taaataatct taagcttaat
    11941 attgaatctt ctgttggtga ttacatttat tatgaaaaat tagaacctgt taaaaatata
    12001 atccacaatt ctatagatga tttaatagat gagttcaatc tacttgaaaa tgtatctgat
    12061 gaattatatg aattaaaaaa attaaataat ctagatgaga agtatttaat atcttttgaa
    12121 gatatctcaa aaaataattc aacttattct gtaagattta ttaacaaaag taatggtgaa
    12181 tcagtttatg tagagacaga aaaagaaatt ttttcaaaat atagcgaaca tattacaaaa
    12241 gaaataagta ctataaaaaa tagtataatt acagatgtta atggtaattt attagataat
    12301 atacagttag atcatacatc tcaagttaat acattaaacg cagcattctt tattcaatca
    12361 ttaatagatt atagtagcaa taaagatcta ctgaatgatt taagtacctc agttaaggtt
    12421 caactttatg ctcaactatt tagtacaggt ttaaatacta tatatgactc tatccaatta
    12481 gtaaacttaa tatcaaatgc agtaaatgat actataaatg tactacctac aataacagag
    12541 gggataccta ttgtatctac tatattagac ggaataaact taggtgcagc aattaaggaa
    12601 ttactagacg aacatgaccc attactaaaa aaagaactag aagctaaggt gggtgtttta
    12661 gcaataaata tgtcattatc tatagctgca acggtagctt caattgttgg aataggtgct
    12721 gaagttacta ttttcttatt acctatagct ggtatatctg caggaatacc ttcattagtt
    12781 aataatgaat taatattgca tgataaggca acttcagtgg taaattattt taatcatttg
    12841 tctgaatcta aagaatatgg ccctcttaaa acagaagatg ataaaatttt agttcctatt
    12901 gatgatttag taatatcaga aatagatttt aataataatg cgataaaact aggaacatgt
    12961 aatatattag caatggaggg gggatcagga cacacagtga ctggtaatat agatcacttt
    13021 ttctcatctc catctataag ttctcatatt ccttcattat caatttattc tgcaataggt
    13081 ataaaaacag aaaatctaga tttttcaaaa aaaataatga tgttacctaa tgctccttca
    13141 agagtatttt ggtgggaaac tggagcagtt ccaggtttaa ggtcattaga aaataatgga
    13201 actaaattac ttgattcaat aagagattta tacccaggca aattttactg gagattctat
    13261 gcctttttcg attatgcaat aactacatta aaaccagtgt atgaagacac taatattaaa
    13321 attaaactag ataaagatac tagaaacttc ataatgccaa ctataactac taacgaaatt
    13381 agaaacaaat tatcttattc atttgatgga gcaggaggaa cttactcttt attattatct
    13441 tcgtatccaa tatcaacaaa tataaattta tctaaagatg atttatggat atttaatatt
    13501 gataatgaag taagagaaat atctatagaa aatggtacta ttaaaaaagg aaagttaata
    13561 aaagatgttt taagtaaaat tgatataaat aaaaataaac ttattatagg caatcaaaca
    13621 atagattttt caggcgatat agataataaa gacagatata tattcttgac ttgtgagtta
    13681 gatgataaaa ttagtttaat aatagaaata aatcttgttg caaagtctta tagtttgtta
    13741 ttgtctggag ataaaaatta tttgatatcc aatttatcta atactattga gaaaatcaat
    13801 actttaggtc tagatagtaa aaatatagcg tacaattaca ctgatgaatc taataataaa
    13861 tattttggag ctatatctaa aacaagtcaa aaaagcataa tacattataa aaaagacagt
    13921 aaaaatatat tagaatttta taatggcagt acattagaat ttaacagtaa agattttatt
    13981 gctgaagata taaatgtatt tatgaaagat gatattaata ctataacagg aaaatactat
    14041 gttgataata atactgataa aagtatagat ttctctattt ctttagttag taaaaatcaa
    14101 gcaaaagtaa atggattata tttaaatgaa tccgtatact catcttatct tgattttgtg
    14161 aaaaatgcag atggacacca taatacttct aattttatga atttattttt ggacaatata
    14221 agtttctgga aattgtttgg gtttgaaaat ataaattttg tagtcgataa atactttacc
    14281 cttgttggta aaactaatct tggatatgta gaatttattt gtgacaataa taaaaatata
    14341 gatatatatt ttggtgaatg gaaaacatcg tcatctaaaa gcactatatt tagcggaaat
    14401 ggtagaaatg ttgtagtaga gcctatatat aatcctgata cgggtgaaga tatatctact
    14461 tcactagatt tttcttatga acctctctat ggaatagata gatatatcaa taaagtattg
    14521 atagcacctg atttatatac aagtttaata aatattaata ccaattatta ttcaaatgag
    14581 tactaccctg agattatagt tcttaaccca aatacattcc acaaaaaagt aaatataaat
    14641 ttagatagtt cttcttttga gtataaatgg tctacagaag gaagtgactt tattttagtt
    14701 agatacttag aagaaagtaa taaaaaaata ttacaaaaaa taagaatcaa aggtatctta
    14761 tctaatactc aatcatttaa taaaatgagt atagatttta aagatattaa aaaactatca
    14821 ttagtatata taatgagtaa ttttaaatca tttaattctg aaaatgaatt agatagagat
    14881 catttaggat ttaaaataat agataataaa acttattact atgatgaagc tagtaaatta
    14941 gttaaaggat taatcaatat aaacaattca ttattctatt ttgatcctat agaatctaac
    15001 ttagtaactg gatggcaaac tatcaatggt aaaaaatatt attttgatat aaatactgga
    15061 gcagctttaa ctagttataa aattattaat ggtaaacact tttattttaa taataatggt
    15121 gtgatgcagt taggagtatt taaaggacct gatagatttg agtattttgc acctgccaat
    15181 actcagaata ataacataga aggtcaggct atagtttatc aaagtaaatt cttaactttg
    15241 aatggcaaaa aatattattt tgataatgac tcaaaagcag tcactggatg gagaattatt
    15301 aacaatgaga aatattactt taatcctaat aatgctattg ctgcagtcgg attgcaagta
    15361 attgacaata ataagtatta tttcaatcct gacactgcta tcatctcaaa aggttggcag
    15421 actgttaatg gtagtagata ctactttgat actgataccg ctattgcctt taatggttat
    15481 aaaactattg atggtaaaca cttttatttt gatagtgatt gtgtagtgaa aataggtgtg
    15541 tttagtggct ctaatggatt tgaatatttt gcacctgcta atacttataa taataacata
    15601 gaaggtcagg ctatagttta tcaaagtaaa ttcttaactt tgaatggtaa aaaatattac
    15661 tttgataata actcaaaagc agttactgga tggcaaacta ttgatagtaa aaaatattac
    15721 tttaatacta acactgctga agcagctact ggatggcaaa ctattgatgg taaaaaatat
    15781 tactttaata ctaacactgc tgaag
//
LOCUS       DAMJVM010000080        15799 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.083, whole genome shotgun sequence.
ACCESSION   DAMJVM010000080 DAMJVM010000000
VERSION     DAMJVM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15799
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.083"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            109..1299
                     /locus_tag="PC523_002667"
     CDS             109..1299
                     /locus_tag="PC523_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2476174.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTKENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALENAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTNIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPSGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            1469..2431
                     /locus_tag="PC523_002668"
     CDS             1469..2431
                     /locus_tag="PC523_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2476175.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALNLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILSKNKLNVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMIIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(2553..3215)
                     /gene="zmp1"
                     /locus_tag="PC523_002669"
     CDS             complement(2553..3215)
                     /gene="zmp1"
                     /locus_tag="PC523_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HDF2476176.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHAESTTIQQNKDILSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNVLNQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAEAFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(3315..3400)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(3585..6488)
                     /locus_tag="PC523_002670"
     CDS             complement(3585..6488)
                     /locus_tag="PC523_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HDF2476177.1"
                     /translation="MRKGNKKALLISLIMILSMVMSTIYPTVSYALELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEEEKQTDNKKPEQMPEKEKPTDNKNPEQTPEKEKPTDN
                     KNPEQTPVGEKPIDSKNPEHTQEDKSTDNKKSEQSLEDEKTLDNKNPEKTPKEEKLLE
                     EENLLKVLEDELNEENEDYGFIVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSIT
                     FKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSIMQGKIGLEAYVKKVDED
                     TNAKIHIETTGKIENGSGDVDVEIKPGDKTEVPDAKGTLKKLVEGRKSTTVFMPVKNK
                     DINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNITQSKNSIS
                     VNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSVFDRGDNY
                     LLTKGHSGATNITQVGQVINYQISVNDDKSPISNVVITDNIPEGMRLTTSGEAGHDFR
                     VVEVPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFFI
                     AYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLSSSDNQ
                     IVKYKINMSTYGVYDAGQVNLLDEVNPALKISNIKYSDNLELKKEAGDGKNTIRLVNK
                     YEFKQIKEGEPVQSWVTFDADFTNVKVGETIQNIAQINGSSPPGVETTKQGYAFEAKK
                     VDALDKNVLSGARFNLEDVSGNIVVKDLVSDEDGVIQSSVKKPGIYYLVEFRPPNGYE
                     KLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVPPNTDEPVVNPPVPPSTDKPKKPS
                     SPSDTDETVVINPPVPPSEDMTNPPIPEVVNPPIPPSEEVIETPVKQIVPIPEVVKPS
                     VSEEKNNKVKDDTLVNPPVPPKTGDNTTIIGEILLVLGAIVGLIVLKRNKNAN"
     regulatory      complement(6601..6686)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(6813..7991)
                     /locus_tag="PC523_002671"
     CDS             complement(6813..7991)
                     /locus_tag="PC523_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2476178.1"
                     /translation="MFSKILVKTFIRDSENVQNRDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPVPIKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEDMVNSIIELLLSYKHIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPVCLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYDCVIVVDKDFI
                     "
     gene            complement(8682..11456)
                     /locus_tag="PC523_002672"
     CDS             complement(8682..11456)
                     /locus_tag="PC523_002672"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF2476179.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAQALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGVIATLLCMFMIIQM
                     QRKGMLILDTSSVLSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITSKIEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAITSEGELNLIGSSRSYMKEDNLVFSGFVGIIDPLREGVK
                     ESIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFAQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRMMKN
                     SRTMNCNSKN"
     gene            complement(11627..12883)
                     /locus_tag="PC523_002673"
     CDS             complement(11627..12883)
                     /locus_tag="PC523_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDF2476180.1"
                     /translation="MLKTTKISDPELYKIVANELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSTVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECKPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIILCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLGT
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            complement(12955..14457)
                     /locus_tag="PC523_002674"
     CDS             complement(12955..14457)
                     /locus_tag="PC523_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2476181.1"
                     /translation="MRALNVIERAGNKLPDPVTIFLLLCIIVVILSAIISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDSSYQGNPAMNLYFAFVSAIILTFAGAF
                     VTEKFIAPRFSKYSDAVNDKDMEELSELENKGIKYALLSLLAIVVIIVALCIGENAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGRVSNKIKNDKELVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKVLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEVIILLLFMSIGIPLGPGGGISYVL"
     gene            complement(14556..15740)
                     /locus_tag="PC523_002675"
     CDS             complement(14556..15740)
                     /locus_tag="PC523_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2476182.1"
                     /translation="MHELISISEKYKEDILDFFTDIHMHPELSFKEFRTTKVIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPICEEVDIEYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEK
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYTIACKSVCDLYSEDAIVDPVPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCMYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KK"
BASE COUNT         4965 a         2734 c         1912 g         6188 t
ORIGIN      
        1 aaaagtttat attttcaaac aaataatttt ccgaaaattg tctaaattca gtaaaaatat
       61 ggtaatatat acataagcaa tataaatcta taaaaaggag tgttacatat gatatctaat
      121 aaaatgcaaa ctttagttgc aaatagttcc gttataagag ctatgtttga agaaggaaag
      181 aaactttctg atatttatgg tgaagaaaat gtttttgatt ttagtatagg aaatccaagt
      241 gttgaacctc cagagactat taaagcagtt ataaatgata ttttaaatga agaatctcca
      301 aatttagttc atggatatat gaacaactca ggatatgagg atgtaagaga tgctattgct
      361 gaacatataa ataaaaaaga tggattaaat cttacaaaag aaaatttaat tatgacttgt
      421 ggtgctgctg gtggtctaaa tattatttta aaaactcttt taaaccctgg tgatgaagtt
      481 attgcttttg caccgtattt tggtgagtat aaaaattata cagaaaacta tgatggaaaa
      541 ttaatagaag ttcctacaaa tatagagact tttgaaccag atttagatgc tctagaaaat
      601 gctattacac caaaaacaag agctttaata ataaacactc caaataatcc tactggtgtc
      661 atatattctg aagaattatt aaaaaatctt ggagaattat tggattcaaa acaaaaagaa
      721 tttaatacaa atatatacct aatttctgac gagccatata gagaaataat ttatgatggt
      781 gccaaagttc cttgcgtact aaaatactat agaaattcat ttataggata ctcttatagt
      841 aaatcattat ctctacctgg tgaacgtata ggatatattg ttgctaatgg acaaatggat
      901 gattttgatg atgttatgtc ttcattaaat gttgcaaata ggatacttgg atttgtaaat
      961 gctccatctc ttttccaaag agttattgca agaagtttag atgctgaagt tgatgtaaat
     1021 atttataaaa agaattttga ccttctatat aatagtttaa tcgatatggg atattcttgt
     1081 gttaaaccta gtggtacatt ctatttattc ccaaaagctc caattgaaga tgataaaaaa
     1141 ttctgcaatg atgcaaaaca atttaattta ttattagtac caggttcttc ttttggttgt
     1201 ccaggtcatt ttagagtatc atattgtgta tcttatgaca aagttaaaag ctctttacca
     1261 gcatttgaaa aattagccaa attatataat ttaaaataat taaatcaaag taattcaaat
     1321 aattaactac cttagtatag aatacttaca ttctatatta aggtagttta taattttatg
     1381 tactatattt tatacatacc atattattcc aatatacttg aatattctta tctatagtac
     1441 taatataaat tacaaggagg tgtttgactt gaaaaaaggt tatgtttata taatactgac
     1501 taccttattt tttagttcaa tggaaattag tctaaaaact gtcactaatg actttaatcc
     1561 cttacaaata actttatcaa gattttttgt tggcggttta gtcttactac cttttgctgt
     1621 aaaaagatta agagctctta atctttctat tacaaaatca gacttaaaat actttgcatt
     1681 tttaggtttt atgtgtgtag tagtaagtat gtcattgtat caactggctg ttctaaacac
     1741 aaaggcatct gtagttgctg ttttgttcag ttgtaatccg gtctttgtaa tgcttttagc
     1801 ttacttaatt ttgaaggaaa aaatatatag gcacaatgta atttcactta tattagaagt
     1861 tcttggaatt atagttataa taaatccatt gcatacaaaa ctaactctta gtggaatatt
     1921 cttaacatta tcttctgcta ttattttttc aatgtatact gtatttggta aaagaaaaac
     1981 cttaaaattt ggtggaatag ttgtaacttg ctttagcttt atatttggta gtttggaaat
     2041 gctgatattg gttttactca ctaaaattaa ttttatcgca gatatattaa gtaaaaataa
     2101 acttaatgta tttgcaaata tacctatttt tagtggatat accttacaca atatgccaat
     2161 tatgatttat gtattcgtat ttgttacagg tgttggttat gcactttact ttatggcaat
     2221 ggaagctaca tctactagtc ttacatcttt agtcttcttc tttaaaccag taatatctcc
     2281 aatactagca ttattaatac ttaaagaaat tatccctata aacatgatta taggaatttt
     2341 attgatagta attggttcaa taatttctat tatacctacg ataattactc aaatgcataa
     2401 taaacatgtt gaagccttgt ataaggagta gtaattgata ctctatcagt ttatacataa
     2461 gacaaaattt attagtttta aacgcaatat aaaagagatg ctattacaat agcatctctt
     2521 aatttatatt tattttaaat ttattaaatt atctatttag ctaggttttg taaaaaacta
     2581 taagtttgtg gacaagctga ttttaactta ctatttgtat cttgatttag atagtaatac
     2641 gcaaatgctt ctgcaaaaaa ttcttctgga tatacaccta agtagtttac attccctaaa
     2701 gaacgtcctt ctttagcaaa tatttgttta aattgtgctg atttagatat atcatttaat
     2761 actatatggt ctattgcatg tgctgtttca tgtaattcta agtttattgc atcatgacct
     2821 ttacctttgt tactaaatcc tattctaaga gctactgtgc ttccacctaa acccggaaca
     2881 tcatcccaag tttttccagt tccttcccat cccttaggaa ctacaccttt taaataagca
     2941 tattcttttt catctgttag ctttccactt aataatttta tttttaagtt attttgattt
     3001 aatacattta aatactttcc atctatatta gctagtctat ttaccatagc attagcttca
     3061 tttttatcgt aatttccagt tggaaaaact actatctgac taagtatatc tttattttgt
     3121 tgtatagtag tactctctgc atgtgcactt actggaactg aacttattaa gaatattgat
     3181 attatagcta ttaataattt tttacttggt ctcatattat tccctccttt gagttaggcc
     3241 gggagtaata aatttcaaaa taaaaaagat acccaagagt atctttgtat atacaaaaag
     3301 aaatttttac tcttttcggc aactggctgg catggtttac taaactttag cccctatagc
     3361 tttgcgtgac taggtttccc tagttttgcc tcatttaatt ttatatctat attatagctt
     3421 atatcttttt aattgtctat gctctttaga atttttttct aaaaagttat tattttttta
     3481 cataaatctt caaaaaaaaa tatcttctaa caatattttc actattaaat attattttag
     3541 aagatatttt ttaatattac ttacattttt tataatgtta gggtctaatt tgcattttta
     3601 tttcttttta atacgataag tcctacaata gctcctagaa ctaaaagtat ttctccaata
     3661 attgtagtat tatcccctgt ctttggtggt actggtggat ttaccaaagt atcatcttta
     3721 actttattat ttttttcttc tgatactgat ggctttacta cttctggtat tggaacaatc
     3781 tgtttaacag gagtttctat cacctcttct gatggtggta ttggtggatt aactacctct
     3841 ggtattggtg gatttgtcat atcttctgat ggtggtactg gtggatttat aactacagtt
     3901 tcatctgtat cagatggact tgatggtttt tttggtttat ctgtgcttgg tggcactggt
     3961 ggatttacca ctggttcatc tgtatttggc ggtactggtg gattttcttg tcttatgttt
     4021 tctatatttc caatatccac tattctacca atatctttat taccaatttc aaccttaatt
     4081 ttatccttca atttttcata tccatttgga ggcctaaatt ccaccaaata atatattcct
     4141 ggttttttta cactagattg aattacacca tcttcatcac taactaaatc cttaactact
     4201 atatttccag aaacatcttc taaattgaac ctagcacctg aaagtacatt tttatctaaa
     4261 gcatccactt ttttagcttc aaaagcataa ccttgcttag tagtctctac tcctggaggg
     4321 cttgaaccat taatctgagc tatattttgt atagtctccc caacttttac atttgtgaag
     4381 tctgcatcaa atgtaaccca actttgcaca ggttcacctt cttttatctg tttaaattcg
     4441 tatttattta ctaatcttat tgtatttttt ccatctccag cttctttttt aagttcaagg
     4501 ttatcagaat attttatatt agatattttt agagctggat ttacttcatc taaaagattt
     4561 acttgccctg catcataaac cccataagtt gacatattga ttttgtattt aacaatctga
     4621 ttatcactac tacttaaaac ctttttatca acttcttttt ttgcgcttat taatccagaa
     4681 tttttcttaa agtttatctc atcattttta tcaatttcac tattatttgc atcaagaaca
     4741 gctctattta tatagctatc ttcaatacta attaccttta atgtataagc tataaaaaac
     4801 tttgattcta cttcttcttt actaagatta attgtaaatc cagttataac tttatcgact
     4861 tgcccacttt catttctttt ttcttctact ttatatgaaa tattgttggc aattttttct
     4921 ttactccaag gagtccagct accattcatt ggaacttcta caactctaaa atcatgtcca
     4981 gcttctcctg aagtagtcaa tctcatacct tctggtatat tatctgttat aacaacatta
     5041 ctgattggtg acttatcatc atttactgaa atctgatagt ttattacttg accaacttga
     5101 gtaatatttg ttgcccctga atgtccttta gtgagaagat aattatcccc tctatcaaac
     5161 acacttacag tagcatcatc ttcttgtatc ttcttcccat cagattcaat cctagctaca
     5221 ttcttatatt tattgccatg ttttgcattt ttgtcactga ttctagcctt atatttaaca
     5281 gtatacgatt tatctatatt tccaaaatta actgaaatag agtttttact ctgtgtaatg
     5341 ttaaaatcac taacttcttt tccatcactt gttactacac ttacagagcc atttatcagt
     5401 gtcaatcctt caggaagctc atcatacaaa ataatatctt ttaactcttc ctgcttttca
     5461 tttacttgta tactgtaatt tatatcttta tttttaactg gcataaatac agtagttgat
     5521 tttcttcctt caacaagttt cttaagtgtt cctttagcat ctggaacctc cgttttgtct
     5581 ccaggtttta tttctacatc tacatctcca cttccatttt ctatttttcc agtggtttca
     5641 atatgtattt ttgcattagt atcttcatca acttttttaa catatgcttc caaacctatt
     5701 ttcccttgca ttatactttg gccattaggt aattctctaa atgtaagtgt atactctcca
     5761 ttactttctg ttttatcgaa acattcactt gtataatcta aattaacttt attgaataca
     5821 tctggaacct taaaagttat tgaatctcct gcttgtattc cttttgaagt tggagtataa
     5881 gttaaattaa atgatatttt tttcattgat tccgtttcta ttgtattgtt attaattttt
     5941 acaataaatc catagtcttc attttcttca ttaagttcat cttctaaaac tttcaataaa
     6001 ttttcttctt ctaatagttt ctcttccttt ggagtttttt ctggattttt attatctagt
     6061 gttttttcat cttctaaaga ttgttctgac tttttattat ctgttgactt atcttcttgt
     6121 gtatgctctg gatttttact atctattggc ttttctccta ctggagtttg ctctggattt
     6181 ttattatctg ttggtttctc tttctctggc gtctgttctg gatttttatt atctgttggt
     6241 ttctctttct ctggcatctg ttctggtttt ttgttatcag tttgcttttc ttcctctgga
     6301 gtttgttctg gttttttatt gtcgctttcc ttttcttctc cagtggatga attagttgaa
     6361 ccactttgaa tctgactatt ctctcctaat tctaaagcat aagatacagt tggatatatt
     6421 gtagacatca ccatacttaa aatcataatt aaactaatta ataatgcctt tttatttcct
     6481 ttcctcatat aaatttctcc cctctaggtt atctatactt tctaatcttc cattaatatc
     6541 atatataaat taatttttac taaaaaatta tacaaaaaaa gctatgaaaa taaataaatt
     6601 tatattcata gccggttgca ccactgactc agtatatcca taaggtgccc aataatagaa
     6661 tatacttcaa catctctata atatttcata aaaacaatta aaattgtttc atttatatta
     6721 aaaatacaat tatatacttc tattatatta tgcctttata ctattgtcta tcttttttac
     6781 attttttacc ttattatgag attaattaat aattaaataa aatccttatc tacaacaatt
     6841 acacaatcat attcaggatt tatctcctta actaattttg ttatatcttc ttttaagtca
     6901 tcctccgaca tgtttttgga aaattcggaa gggttaacta ctatatcaaa tattaaattc
     6961 ttcttgctcc cctttccaac tattctaaaa tcatgcatag attttacaag gctatttttc
     7021 ttaagtattt cttcaagttc ttttttggct gacataactt ctttatcttc aagacatact
     7081 gggtccatgt gaattactag atatatatct aatttttcag atatttctct ctctgcagta
     7141 tctataattt catgtatttc cattatatct atgttggatg gaatttctgc atgtatagat
     7201 gcaatacatc taccgactcc gtagttatga ataattaaat catgtgtacc tattatatgt
     7261 ttataagata acaatagctc tataatagaa tttaccatat cttcatctgg tgcttctcct
     7321 agaagaggat ttatagtatc ttttacaaga gagaaaccag cataaagtat cactaatgat
     7381 actacaatac caacatatcc atctatagga aaattagtaa atctagctac aataaacgat
     7441 ataactacac aacttgaagt aaatacatct cctaaagcat caagtgaaac tgcttttaat
     7501 gctgaagaat caattttatt tcccataaat ttattaaaac ggctcaacca gattttaatc
     7561 attatagatg caattaaaag tataagtggt attacttcaa attttatagg aactggattt
     7621 actatccttt ctatagatga ctttacaaat tgaagtccaa ccaacatcac cataaaagct
     7681 actattaatg ctgataaata ctctattctt ccatgaccaa aaggatgctc tttatcagct
     7741 ggcttacttg ctaatttaaa acctatcata gttattgctg aagatgccat atcagaaaga
     7801 ttattaaatg catcagccat tatagctata ctggatgtga gcattcctat gaatactttt
     7861 atcacaaata aaagtaaatt ggataatatg cctactacac cagccacata accatattta
     7921 tttctaacat ctctattttg aacgttttcg ctatctctaa taaatgtttt gactaggatt
     7981 ttggaaaaca tgaaacttac ctccttgtcc tttattttat taacaaattt tagaatctat
     8041 atctttattt atgataacat aatttatata taaaattctt aaaaaattac tatacttcaa
     8101 taaaatatta ttatatatca atttaaatac aaatcattat atttagcaaa gtttttatgc
     8161 cttaatatct gcttaataca ttctatgtca ttttttaaaa agattattta ttacgataat
     8221 atcatttttc ccaagtattc cttagaatat gattttttta ttttgtctat acatatttta
     8281 aaattatact gtatttatct atattttata accttatgat acactataac tataagattt
     8341 taaacggaaa ggaatgaaat aaatcatgtt ttttatcagt gatatttaca caaaatcacc
     8401 cattaaattt gatacttcat tgcaaaaaga ggtatataaa atattacaaa agttaaatat
     8461 tgacttttaa catattgaca cagatgaagc aattataatg gaagattgtg tttagattaa
     8521 taaaaaatta aatatgaaaa ttaaaacaaa acaaatcatt catgatttct tatcttttgc
     8581 aggtcataca ccatctataa ttgaattgta atataaaata gcataaaaaa aacacaaacc
     8641 gcaaagtcac aatatattgg tactttgcgg tttcataaga attaattttt actattacag
     8701 ttcatagttc tactattttt catcattctg agtattagct ttacaaattc atttgacaat
     8761 aggatagttg atgcaaatag tattattttt atccacatat caaaatccaa agaaacagaa
     8821 ttaaagaaac tctggaacac ttgtgtaaat attatttgaa ttatccctgt aactactatt
     8881 atttgcaatg ccagtttatt tttaaagaaa tttggtattg tactattcaa accaaactct
     8941 ctacaattta atgcattaaa aagggcactg aatgcaaata aagaaaatat aacagtacct
     9001 tgttcttctg atgtagctcc caatatattg aaagctgatt gagcaaatac aattgtaatt
     9061 ataagaatag catttataat aatatttaca aacatagacc tagctattat tccagaatgt
     9121 ctatttattg gctttctttt caatacataa tctctaacag gttctaatcc taatgctaag
     9181 gctggaggac catccattat tatatttacc cataataatt gtattgtagt aaatggcata
     9241 tccttaccag taagttgaga tattatagca attataaatg ctactatatt tacagtaagc
     9301 tgaaactgta taaatctttg aaagttctca tatatacctc ttccccattt tattccctct
     9361 actatagtac taaaactatc atcagtcaat attatatctg ctgcattttt agatacttct
     9421 gttcctgaaa tacccattgc aattccaaca tctgcttgac taagagctgg ggcatcattt
     9481 ataccatcac ctgttacagc tacaacttcg ccactttttt gaagtgctga aactattctc
     9541 atttttgtat ctggtttact ccttgcaaca atagatattc ctttgatttc ttctctcaat
     9601 tctttatctg tcagtacatc tatataggtt gcttcaactg cctttttacc atcatttaat
     9661 aatcctaatt catttccaat agctgttgct gtattgatat tatctcctgt aagcattttt
     9721 acatctactc ctgcattaaa tgctttatca atggactctt tgacaccttc tcttaatggg
     9781 tctattattc ctacaaatcc actaaaaact aaattatctt ccttcatgta acttcttgag
     9841 cttcctatta agttcaattc accttctgaa gttattgcaa cttctgtttt agaattactc
     9901 atttttttat aagcaaatcc taatgttctc atagatttaa tttgtaattt cttaatctca
     9961 tctaaaatac ttttctctat ttttgaagtt attggaacaa tattttttcc ttgttgcaca
    10021 taggaacatt tttttaataa tacttctggt gctccttttg aaagcaatat atctgaatcc
    10081 tcttgacgaa ttaatgttga catcttcttt tcttctgaac taaatggtat ttgtgatatc
    10141 aaatacgttt gttttctcat ttcattatag ttcttatcat tatgatataa aagtaatgca
    10201 cactctgttg cacttcctat atatttaaat gaattatctt ctttttctat atctgcggtt
    10261 gaatttacta tacagttttg ttcaaaatag ctgttacttt gatactctcc tccacttata
    10321 tatttaccat caacatatgc tacctctacc atcattttgt tttgtgttaa agtccctgtt
    10381 ttgtcagaac atattacaga aacagaacct atagtttcac aggcttcttt ctttgttact
    10441 agtgcattta tctttgccat cttttgcatt gttatagcta aagtaatgtt tatcatagtt
    10501 ggcaaacctt ctggaacagt tgcaactatt aaggcaatac aaaccatata tgcattttta
    10561 gcaggttcta atgattgtaa gaaagataat acactgcttg tatctaaaat taacatacct
    10621 tttctctgca tctgtataat cataaacata cacaataaag tagctattac acctgatatt
    10681 cctgctattt tagctcccaa tttccctaat ttaatctgaa gtggagtttg ctggtcttca
    10741 tttgataaat tctgtgctat cttacccatc tctgtcttat ctccagtatg agtaacgacc
    10801 attatgcctc taccatatgc tacaagagtc cccccaaata ccatatttac ttgctttgca
    10861 ggtattgcat cctgttcgat tatttctgtt ttagaataaa caacttccat atctaataca
    10921 gcatcagcat tcttagctac atcatcagat tctcctgtta acatatcttc tctaagtttc
    10981 aaatttatag attgtataag tcttccatct gctggtatca tatctccagt ttctatatat
    11041 acaatatcac ctggtactaa atcatttttt gatattttaa ttatcttccc atttctaagt
    11101 acttttactt ctatattttc tgttaatttt gacaaggctt gagctgcttt tttagatttt
    11161 ccttcagtta ttatacctat agaaattcca ataaaaatag cccctaaaat acctaatgca
    11221 tcatgtagtt ctcctacaaa agaacttatt actgctgctc ctatgagaat taatatcata
    11281 ggctctgtca aactttctcc caactcgtcc caaaatgttc taccttcttt tatagtaaat
    11341 tcatttaatc catatctaag cttcctttct tctacctcat tatcatcaag tccaatttct
    11401 ggatttgttt ttaagtattt taagacttct ttggtaggtt tattataata cctcataact
    11461 ttatccccct tgttaattat ctataaggaa ttatatgctt atgatagctt gtatattact
    11521 tttcaaaaaa ataaggtgtt gattaatcaa caccttatag tggttatata ttattaaatt
    11581 ttatgttata ggctttataa attgtgagtt atataaatca aaatatttaa tcttcaaagt
    11641 acccttctgg atataatggg aacttagata taagttcttg tgcttctgct ttacattcat
    11701 ctaatacttc cttattatca atattttctg ctaccttatt cattattcct gctatctgta
    11761 taacttcttt ttctttaaga cctctttgag atattgatgt aagacctata cgaacaccac
    11821 tagttacgaa tggtgattgt gggtcatcag gtataacatt tttattaact gttattccta
    11881 catattcaag agctttttca aattgttttc ctgtaagatt cttgcttctt aagtcaacca
    11941 ttactatatg attatcagtt cctccactta caatactaaa accatgtgaa atcagttcag
    12001 aggctagagt ctgagcattt actacaactt gttccattat ctttttaaat ttatctgtac
    12061 ctaaatacaa tagtgaaaag gcttttgctg ccactgtatt aagatgtata gaacctaatg
    12121 ttcctggaaa aacaccttta tcaagtttct ttgcatgttc tgctttacaa agtataatac
    12181 cacttcttgg tccacagaaa gtctttgttg tagatgaaga aacaaaatct gcataaggta
    12241 cagggctagg tattacttta gctgcgacta atcctgcaac atgtgccata tctaccataa
    12301 agtaagctcc tacttctttt gcaacctttg atattctttc ataatctatt aatcttgggt
    12361 atgaacttgc ccctgcaatt ataagttttg gtttacattc ttttgccttt gcttcaagag
    12421 catcataatc tatacgtcca gtatttggat ccaccccata aaattcatat ttataaatat
    12481 catgtaaaaa attaactgtg ctaccatgtg ttaagtgtcc accttggtca agacgcatac
    12541 ttaagacagt gtcatttggt tttaatattg atgaatatac acaatagtta gctgtagagc
    12601 ctgaatacgg ttgtacgttt acatgttctg ctccaaatac ttcttttgca cgttttattg
    12661 ctagagtttc aagtttatct gcttcttcag aacctgcttg aaaacgagcc cctggatagc
    12721 cttcttctgt tttatttgta aatacacatc cacttaattc aagaacttct gttggtgctg
    12781 tactttcaga agctatcatc tcaatattat gttcttgtct aaccaattcg ttagctacaa
    12841 tcttgtataa ctctgggtct gaaatttttg ttgtttttaa cataataatc ctcctcttaa
    12901 ttaaagaata tattttatta gattaatctt tattaataaa tttattataa tggtctataa
    12961 aacgtaagat attcctccac caggtcctaa tggtatgcca atactcatga atagtaacaa
    13021 tattattacc tctattaatg taaatactac tgagtatgga atcatattcg atatgacagt
    13081 gcctatacct atatctttat catatcttct ggcaaaagct aatataactg gaaaatacgg
    13141 aaaaagtgga cttattggat ttgttgaagc atctccaata cgataagcta tttgagttaa
    13201 tgctggatta aaatctagta gcataaacat tggaacaaat ataggtgcta aaattgccca
    13261 tttcgcagaa gcacttccta caaataaatt gataaatcca gataacaata taaatcctat
    13321 tatcaatcca taactaggta tccccgactc tgccaatact tttgctcctg ttattgataa
    13381 aattgttccc aagttactaa gattaaataa attaataaat tgagatgcta taaatgctag
    13441 aactatatat cctcccatat cactcatact actacccatc atagatacta attctttatc
    13501 attttttatc ttattactta ctcttccata tactaaccct ggagttaaaa atatgatagt
    13561 aattattgga actattccct tcattaaagg tgcattgctt gataatatag aaccagtttc
    13621 tgggtctttc ataaatgcat tttctcctat acataaggca actattatta ccacaatagc
    13681 taatagtgat aatagagcgt actttatacc tttattttca agctcactta attcctccat
    13741 atccttatca tttactgcat cactatattt tgaaaaacga ggagctataa atttttctgt
    13801 tacaaatgct cctgcaaaag tcaaaattat tgctgataca aaagcaaaat aaaggttcat
    13861 tgctggattt ccttgataag atgaatccat catttgagct gcaggtattg taaatccagc
    13921 taataataca tctgtagtac taagcataac atttgctgca aatcctcctg caacccctgc
    13981 aaaagctgca aacataccta caagaggatg tcttccaatt cctaaaaaca caagtgctgc
    14041 aagtggtggt aatattataa atcctacatc tgctgctgta tgtgataaca tacctgcaaa
    14101 tacaattgct acagttacaa gtttttgtgg aactttactt atcccttttt tcattaacac
    14161 ttccataaat ccagatttct cagcaacacc tgctccaagc attgtaacca gcactaatcc
    14221 taatggagca aatgattgga agtttgtaac tatactacct aagtatgatt gaatctgttc
    14281 tttctctaat agatttacta cctttacaac ttcttttgta gaaggatgta tctcttctac
    14341 tcctaaatta gatataattg ctgacaatat tacaacaatt atgcatagta ataaaaatat
    14401 ggttactggg tctggaagtt tatttcctgc tctctctata acatttagtg ccctcattaa
    14461 taatccacta tctttgtttt tttcctgtga attaattttc ctcaaactaa tcccccctca
    14521 taaactttat ctccagaaat cttctatctt aaattttatt tttttaattt atccatataa
    14581 tttataacag actgacatag tacatttgta cctaccaata tggaattttt atcagcttta
    14641 aatttaggat tgtgccactg atacatacaa tcttgctctt tgttgcctac tcctaaccag
    14701 taaaaaaatc ctggagcttt ttccataaat atagaaaaat cttctcctcc tgtagaaggt
    14761 actggatcaa ctatagcatc ttctgaatac aaatcacaca cagacttaca ggctattgtg
    14821 tacatctctt tactatttat aacggctggt aaatcaaata aataatctaa ctttgcagtt
    14881 gcttgatatc cacgagctat ctcacaaact attttttcta aattttcaga tattttcttt
    14941 cgtacttcat tactaaagct tctacatgta cctgtcatat ctactttttc tgaaattaca
    15001 ttatttgcag ttcctccatt tatagtacct attgatataa cagctgattc taatggactt
    15061 atgtttctac ttactatggt ttgaatgccc attataattg cacttgatgc tactatcggg
    15121 tctattgttc tgtttggtat acctccatga cctccaaatc catttacttc tatcttaata
    15181 gtatctactg ctgccattaa cccacctaac ttaacaccta tttttccaca aggtatatct
    15241 ggatgattat gaagaccaaa tatagcatca acttttggat tttccattac tcctcgttct
    15301 actaacatct ttgcaccttt attgacttcc tcggctggtt gaaatataaa ttttacattt
    15361 ccatgaagac tatccctaat atgagacaat acatatgcac atcctacaag gcaagatgtg
    15421 tgtatatcat gtccacatgc atgcattata ccatcatatc tggatttata ctcaatatca
    15481 acctcttcgc atattggcaa tgcatcaata tctcctctta gtgctactgt gggaccatca
    15541 tacttccctt ttaataaccc aacaactccg gtttccattc ctaagtctaa tatttctatg
    15601 tctaaactta ctaataaatc ttttatcact tttgtagttc taaattcttt aaaacttaat
    15661 tcggggtgca tgtgaatatc tgtaaaaaaa tctaaaatat cttctttata cttttctgaa
    15721 attgaaataa gttcatgcat actaacacct ctttaaaatt ttattttttg attttttgat
    15781 aatttaacta taaattata
//
LOCUS       DAMJVM010000081        15692 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.084, whole genome shotgun sequence.
ACCESSION   DAMJVM010000081 DAMJVM010000000
VERSION     DAMJVM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15692
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.084"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            219..1649
                     /locus_tag="PC523_002676"
     CDS             219..1649
                     /locus_tag="PC523_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2476183.1"
                     /translation="MSSQESKPKGNFISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAIAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIGDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            1752..2855
                     /locus_tag="PC523_002677"
     CDS             1752..2855
                     /locus_tag="PC523_002677"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDF2476184.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAMEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENKKFVAVLKEILNIKEEPSIG"
     gene            2870..3106
                     /locus_tag="PC523_002678"
     CDS             2870..3106
                     /locus_tag="PC523_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2476185.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            3123..4172
                     /gene="vorB"
                     /locus_tag="PC523_002679"
     CDS             3123..4172
                     /gene="vorB"
                     /locus_tag="PC523_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2476186.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREEDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RISENEQQWEDYQIEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4174..4914
                     /locus_tag="PC523_002680"
     CDS             4174..4914
                     /locus_tag="PC523_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2476187.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSANGRDISTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWSV
                     SLEKSIKWMEEEIVPYYTLGEFKQRDGE"
     gene            4917..5456
                     /locus_tag="PC523_002681"
     CDS             4917..5456
                     /locus_tag="PC523_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2476188.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENIA
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNIQNEAAFMEGYNNAI"
     gene            5580..7679
                     /locus_tag="PC523_002682"
     CDS             5580..7679
                     /locus_tag="PC523_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HDF2476189.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKDTDRVYYVN
                     PKREELFGRKCYKTIQEVPEIVDCVVVCTPRNVVPSVLKDSAELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDNYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPTNSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDSSVGFTMVGANIRDEE
                     NEMHETLCQAVSEAREQGLKKPVFAVPTLEGNRYLDYRNRLEYNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(7946..8461)
                     /locus_tag="PC523_002683"
     CDS             complement(7946..8461)
                     /locus_tag="PC523_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HDF2476190.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YSAPTNPPVTI"
     gene            complement(8553..9764)
                     /locus_tag="PC523_002684"
     CDS             complement(8553..9764)
                     /locus_tag="PC523_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDF2476191.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIKEIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVAFIKELSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTVSASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
     gene            10422..11864
                     /locus_tag="PC523_002685"
     CDS             10422..11864
                     /locus_tag="PC523_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2476192.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGSI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPSETKQRLKNVKKIY
                     IIGKELSISKSVENELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSVAQNNGDSSTKGDSPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            11906..12259
                     /locus_tag="PC523_002686"
     CDS             11906..12259
                     /locus_tag="PC523_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476193.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFVTDIFNLADVQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTSWILSLIVSLLTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HIAAKFYYKIKTFKN"
     gene            12449..13270
                     /locus_tag="PC523_002687"
     CDS             12449..13270
                     /locus_tag="PC523_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDF2476194.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNIPVPSKAYFKGLSKEDAYYKYFNSILETNREFRDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDIIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFINIEKVKSNNIA"
     gene            13528..15411
                     /locus_tag="PC523_002688"
     CDS             13528..15411
                     /locus_tag="PC523_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDF2476195.1"
                     /translation="MSNKNKDNITLSNQIKRTTQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIIMNKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQKEKILFTEKRIIELGNENDEKELCEISNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDSSINPEGTDEWGATPTRENFTGGDLQGV
                     IEHLDYLSDLGISGLYFCPITIGKTNHRYDTIDYMEIDPALGDKRTLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKDWFYIKDMNKIDIPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIEYLLDVGKYWIHEFDIDAWRLDVSNEVDHAFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVNEVIFNLLGSHDTTRILTYANGNVDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNRDIFNFMKKIIRLRKENKELRSISNEWVLADKE
                     NGAIILKKESIFIIINNSSKDIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
BASE COUNT         6069 a         1780 c         2827 g         5016 t
ORIGIN      
        1 ctaaattaag cttaaatggc tttttagtta attttaatag taaaaattaa acttaaaaaa
       61 tatttgacaa ttatgaattt tgagaatata attgaattaa aattagacac atataaaaat
      121 atactctgaa tcaagaagga tatatttaag attcaaaata tatattatta tacgtgaata
      181 ataaaaaagt aagcaaattt tataagggag gggtagatgt gagtagtcaa gaaagcaaac
      241 caaaaggaaa ctttatatca aattttagag atgatatgag agaccctaag aaaagagcaa
      301 tatacacata ttcaagttct ataaacctag cgtacaattt tataggttac tttgtatcaa
      361 ctgttacaaa tatcttttta accgatgtgg caaaacttgg tgtaattatg gcaggtggta
      421 tgcaaatcat acaatctttc ataaaaattg ctatagctcc tataacaggt acagtatttg
      481 acaaacaacc attcaaaaaa ggaaaatatt atccatggct tagatatgca ccagcagcac
      541 tagcattaac gtatggaata tttttcatag ttccattaat gaaaataagt ccagcattac
      601 ttttaccttt gatgacagct ttattattaa tagcatcttg tgctcagcaa gtagttttca
      661 ctataattta tgctatttac cctgtagttg ctaagacacc tagagacaga gttataggtt
      721 caactactac gaatatattc aaagaagttg gtaaattctt agtaggtttt tcatatccgt
      781 tattattagt tttatttgca gatgtattag gtggagaggg aatgggttat ttaggtacct
      841 atttattctt tgcagcagga tgtcttttag tattttggtt ttcttcaaca gagataataa
      901 aatctggagc agaagaagaa gttcttcaaa aagaagagaa gccaaagatt aaagcaagtg
      961 aaatggcaaa agctttattc actaatgtac cacttatgat ggcattttgt ctagaatttc
     1021 ttatatgtat aagatctata ggattaggac ctttggctcc atattatttt aaatatgtaa
     1081 taggggatga acgtggatta gcagtatttt tatcaatcat gcctttggta agtgtagcgt
     1141 tcatgttttt tgcaccagta ttcataaaaa tctgtagaga gcaaaaacta gcttcgataa
     1201 tttctttttc tatatgtgca ttatgccact tattggttgc atggactcct tgggggaaaa
     1261 ctactttagg agtaactatg ttgctggcaa ttggtggagg tttttcaaat gtagtatcaa
     1321 taataaatct taattttttt gcaggttctt gtgattatgg tcactggaaa tcaggaaaag
     1381 acttacctgg tttaagtatg tcattatatc cagttgcaat acaagtagga gtattacttg
     1441 caactactat aagaacagtt ttaatgaact caatgggata tcaagctgat atggtagtca
     1501 cagaagcagt taaatcaggt tttataaata tgatttctta ttctatggca atacctttaa
     1561 taatagctgt tgttatagca atactatatc cagtaagtga caagaaatta aatcaaataa
     1621 gagaagaact aaatcaaaga aatgcttagt taaaagaaag ttaagtagat gactaattta
     1681 agttattaaa gtaagtagac gcaatagcgt ttatataaat gaaaatacat atacagaggg
     1741 ggcttttaaa aatgatagaa aaattattga gaaatacagt aaaagaatta catcaatatg
     1801 ttccaggtga gccaatagaa aaagttaagg aaaagtatgg tgtaaaagaa ataataaaat
     1861 tagcttcaaa tgaaaatcct ctaggtccat caccaaaagc aatggaagct atgattgaaa
     1921 tgttaaaaca aggtcaactt tatccagaac cagaagctaa cgaacttaga agaaaattag
     1981 ctgaaaagct agacttaaaa ccagaaaatt ttattgttgc taatggtgct gataacgtta
     2041 tcacactaat aggtgaggct tttataaata gaggagatga agttatatat tgtaacccaa
     2101 catttccatc atatagaact gcaactataa aaaatgaagg gataccagta gaagtacctc
     2161 taacagaaga ttataagtat gatttacaag gtatattgga taaaattaca gataaaacaa
     2221 aattaatatg tgtatgcaac cctaataatc caacaggaac aattgtagat gataaagagt
     2281 tagaagaatt tttaaagaaa gttcctgaaa atattataac tatattggat gaagcatata
     2341 tagaattttt aacagttcct aattatgtgg atggacttaa gtatgtaagg gaaaattaca
     2401 atgttatagt aacaagaact ttctctaaaa tatatggtct tgcagggctt agagtaggat
     2461 atggtattgc taaagatgag ataataagaa ctctatttac agtaaaagag cctttctctg
     2521 caaaccgtgt tgctataagt ggagcaacag cagcacttga tgatgaagag tttataaaag
     2581 aaacctatga attaaataga gttggtatgg cttactttaa agaagagttt acaaaaatgg
     2641 gatttgatgt tgtagattca caatcaaatt tcctttatgt agatatgaaa acagatatac
     2701 ctaagttatt tgaagacttg aaaaaaagag ggtttgtaat aagaccaagt gctacacatg
     2761 cacgtgtaag tattggaact atggaagaaa ataagaaatt tgtagcagtt ttaaaggaaa
     2821 tattaaatat taaagaggag ccaagtattg gataatagat agaaatatta tgaaacgggg
     2881 tgtagatatg aacttaaaaa taaacgcaga gagatgtaaa agttgtgaat actgcgttat
     2941 atcttgtaaa aaaggagcat taaaaatatc aagtaagata aataaagaag gttatgctca
     3001 tgtagaagtt gatgagagta aatgcatact ttgtggaatt tgctatcaag tgtgtccaga
     3061 taatgttttt gaaataatta aagaagaagc atgtcaagag gcttagaagg agttgggata
     3121 atatgaaaaa aatgtggaag gggaaccacg ccattgcaga agcagcccta agaggtggtt
     3181 gtgaatttta tgcaggatat ccaattacac cgcaaacaga ggtaatggaa tttttatcac
     3241 atagaatgtc tgaacttgga agaactttta tacaatctga aaatgagatg gctgctatat
     3301 atatggtata tggagcatat gcaagtggaa tgaggtctat gacgagttct tcaggaccag
     3361 gaatatcttt aaaacaagaa gggatttctt acttatgtgc aaatcattac ccttgtgtaa
     3421 tcgtaaatgt acagagatgg ggacctggtc ttggttcatt ggattcagca cagacagact
     3481 acctaagaga tactagaggg ggaggaaatg gagattatca tttgatagtt tatgcaccaa
     3541 acagtattca agagactgta gatttgatgt ataactcatt tgatgtagcg gaaaaataca
     3601 gagtacctgt tgaaattttg acggaagcag ctttaggtca aatgatggaa ccagtagaat
     3661 ttccagagtt taagaagaga gaagaagatt taggttggac ttacgatgga agtaatagag
     3721 accatgcaaa ggttgcagat aaccaaaagc caactttttg tatggagaaa cgtatgagaa
     3781 taagtgaaaa tgaacaacaa tgggaagatt atcaaataga agatgcagaa tatgtgtttg
     3841 tagcgttcgg tataccatca agaacaacta tgaatgctgt tagaagactt agagaacaag
     3901 gtgaaaaagt tggaataata agaccaataa cagtttggcc tttcccatat aaagcatttg
     3961 aaaaggtaag caaagatgta aaaggattta tatcagtaga atcaacagac acaggtcaat
     4021 tagtagaaga tgttgcttta gcatcaaaga aagtatgcaa agaaaatgta cctgtgtatg
     4081 gtctatttag tggaaatcat ataccaaaaa cattgcaagt aatggacact tatagcaaaa
     4141 taaaatctgg cgaaatcaag gaggtgttct aatatggcta cagaaaaatt aacacctagc
     4201 gttgtttcaa taccaaacaa gttttgccca ggttgtggtc atggaatagt aaaccgtata
     4261 atagcagagg taatagaaga gcatggatat gaaaaaaatc atgtgttgac attaggagtt
     4321 ggttgtgttt gtaatatgaa ttttagctgg aatggtgata agatgcaaac tgcacatggt
     4381 agagcttcat ctacagctat aggagttaaa gtagcacttc cagatacact agtgatgact
     4441 tatcaaggag atggagatgc ttatgtcata ggtctttctg aaactttaaa tacagcatat
     4501 agaaatcata atgtgactgt atttgtaata aataataata attttgcaat gactggtgga
     4561 caaatgtcat ggaccactat gccaggtcaa gttacaacaa caagtgcaaa tggaagagat
     4621 ataagtacta ctggtagtcc actaaaagtt cctgagatgg tggcaaactt ctttgatgta
     4681 gcgtatgtag caagaggctc agttcatagc ccaaaagaaa taattaatct aaaaaaatat
     4741 ataaaaaatg caatagaagc tcaattaaat ggagagggat attccttagt tgaaatatta
     4801 gcgccttgtc caactaactg gagtgtatca ctagagaaat ctatcaaatg gatggaagaa
     4861 gaaatagtgc catattatac tcttggcgaa tttaagcaaa gggatggtga atagttatgg
     4921 aaaaagagtt agtatttgca gggtttggtg gtcaaggagt tttaactctt ggtttgattg
     4981 tagctgagtc agcacttgaa ttgggtaaac aagtaacttg gatgcctgca tatggtccaa
     5041 ctatgagagg tggaaaagcc tatagtgtag taaagttttc tgatgagagt ataggtggac
     5101 ctgatatgga agaaatagat gttttagtag caatgaataa accatcttta gattatataa
     5161 atttagtcaa ggaaggtgga acagtagtta ttaatacatc tgcaatagat gagaatatag
     5221 cattaagaga agatataagt gtagttaaaa taaattgtca agaattagct caaaaagtaa
     5281 ataatccaaa agcagcaaat atagtagtac ttggagcatt aattgctaag actggattgt
     5341 ttgaaaaaga attagcttta aaaactatgt gtgatttctt tgaggaaaaa ggaaaaggaa
     5401 agtttaatat tcaaaatgaa gcagcattca tggaaggcta taataacgcc atttgataaa
     5461 ttacccttat cttaagtgat ttatctcgag atttaataat ctttagtgcc aagagaaatt
     5521 tcatcttgag ataaaattca cttaaaatca caactataaa catataggag cgtgaattta
     5581 tgagtttgga aaaacttatc agaccaaaaa gtattgctat agtaggagtg actgataaac
     5641 ttggttttgg gagaagtgca gcacttagta tagtaaaaag taaggacaca gatagagtat
     5701 attatgttaa tcctaaaaga gaagaattgt ttgggagaaa atgttataaa acgatacaag
     5761 aagttccaga gatagtagac tgtgtagtag tatgtacacc tagaaatgta gttccatctg
     5821 tattaaaaga ttctgcagag ttaggaacaa aggcagcagt agtatatgcc agtggatttg
     5881 cagaggaagg tacagaggaa ggtacagatt tagaaaatca acttatagaa atatcaaata
     5941 catacgatat gaaaatattg ggtccaaact gcatgggatt gctaaattgt atagataaag
     6001 ttaatttgtg ggcaggaggt tctaaatggg atttagatac taaaaagcct ggtataggaa
     6061 ttgtagggca gagtggattt ataactgctg agattgtttc aagtgattat tttaatatat
     6121 cttatggttt ttcaacagga aatggaaaca tagttacact agaagatgca gtagatttcc
     6181 ttgtagatga taattatgca tcagtaatag caatctacct tgaaggttta aaaaatcctc
     6241 aaaaatttat agatgcttta aaaagagcag cacaaaaaag aaagccagtg ataatcttga
     6301 aatcaggaag gtcagaaaaa ggagctattt ctgctgcttc tcatacagga aatttagctg
     6361 gttcaagtaa ggcttttgaa agtatttttg aaaaatatgg agtaatttct gtagaaaatt
     6421 tagaacaatt tatgtgctta gcacaagctt ttagtgttct agatggcaat ttacctacaa
     6481 attctaattt tgcagcaata aacttctcag gaggagaaaa tacgatttgt gcggatttag
     6541 cagaagaaaa tggtgtagaa cttgcagaga tatcttctga aactaaagaa gaaatgaaaa
     6601 aatatcttcc gggatttgca acacctaaaa atccactaga tgctacaaca gcattattcc
     6661 acgaaaagga tatgatagta ggtctattac atacctttga aaaggacagt agtgttggat
     6721 ttactatggt aggtgcaaat atacgagatg aagaaaatga aatgcacgaa acactatgtc
     6781 aagctgtaag tgaagctaga gaacaaggat tgaaaaagcc tgtgtttgca gttcctacac
     6841 ttgaaggaaa tagatattta gattatagga atagattaga atacaatcaa gtgcctataa
     6901 tgtcatcagt tggaactaca tttacttgtt ttaataaaat ggctaagttt atagattatg
     6961 attattcaaa aaggacttta gaatttaaag ctgttaagaa aagagagtca aataatgtag
     7021 tagcattatc agaattagat agtaagattg agatgaagaa atatggagta ccagttccag
     7081 gacaaggtaa tgcaaaaagt attgatgaat tagatgaaat cttaaaatat attaaatatc
     7141 ctgttgtttt aaaaataaat tcttccgaaa tcttgcacaa atctgatgtt ggaggagtca
     7201 aaataggaat taagaataga gatgaggcag tagatgcata taatgagatt ttaactaatg
     7261 taaaaaaagc aaaaccggat gctaatattg atggtatatt agttcaagaa atggtagaaa
     7321 gtggtataga aattattatt ggtataacta atgatgacca atttggacca atgttgctag
     7381 ttgggttagg aggagtcttt gtagaagtat ttaaagatac tacattatat ccattaccta
     7441 taaatcacga tgaagctatt atgatgctta aaaaactaaa atcatttaag cttttaaatg
     7501 gttatagagg aagtgagcct tgcgatattg atgctcttgc agatatgatg gtcaaattag
     7561 gaaaatatgc ttacgaaaat aaggatgaag tcaaggaaat agacttaaat ccagtgtttg
     7621 tatatcctaa aggaaaaggc gtatgtgcag tagatgcctt aatagttaaa tataaatagt
     7681 ttataagttt tggtacttaa ccataattaa agcactgact gaaaatcaca ttttgtaagt
     7741 taaatagtgt gattttcggt caccccaaat ttactttaaa aaaattttaa agtaaaagta
     7801 tagttttaag tttcattgtg aaatagtact gtaaaaacaa taaaatacta catataaaac
     7861 gaataataaa aggtgtttta atgataagaa tcaattttga ctttcactaa aacaccttct
     7921 ttattatatg aaaaattttt aaatgttata ttgtcacagg tgggtttgtt ggagctgaat
     7981 atgtacgatt agcaaattct tggtcaacaa agaatagacc accttctcca gcaagtttaa
     8041 cacgttttat attgtcttca caatttgttt cttcttctgc ttgttcagat ataaaccaaa
     8101 gtaagaattg agaagttcta aagtctgact cagagtttgc tacagctgct aaattgttta
     8161 ttaaagctgt aacctttttt tcatgagcta acgtacgctc aagaatatcc attgcagaag
     8221 taaagtctga atctggcatt gggatagcat caagctgaac ctttcctcct acattgtgta
     8281 agtatttgta aaaaaacata gcgtggtctg tctcttcttt atattgaaca taaaaccaat
     8341 tagaaaatcc tttaagtcct tctgcttcaa ggtaagaaga catagaaaga taaagatatg
     8401 cagaatataa ttcatggtta atctgctcat taagtaattt ttccatttta gctgaaatca
     8461 tttttatacc tccaaatttt aaatgctctt ataaaatata ataatattaa atggaaaaat
     8521 attcaataaa agtgtttaaa tattttacat atttaataaa aatttatact accattaaaa
     8581 aagttataac ttacaatggc tatagagccg attaatggtg catttgctcc aaaatttgaa
     8641 tgtcttacac ttattttttt atatttagaa aaacttgctg aaacagtcaa tttttttaat
     8701 aatgtatttt ctataaatgt accaggaata ttaaagtcat agcctattat tatagaacta
     8761 caatcaatca acgtaagtgt attaaccaat gcatatgata cgtatgtaca aaattcatca
     8821 agcaaaaaca tagcgagact atctttatta tttgcctcat ctattaattt gactaaagaa
     8881 aaatctttac agttaattaa cggggaatta ggataaatat ggctcaatga ttcaattctt
     8941 ttaattagat tactgacact tgtataataa tctaaacatc cattgttacc acaactgcac
     9001 aatggtccac taaaattaat tgatgtatgt cctatctctc cactttgccc tagattgcca
     9061 gtatgaagtt tattttctag aactagacca gctccaatac cattcattat gtgtaggtat
     9121 atataatttg atatattttt acctaagcca tacatttttt ctgccaatgc gcccgaatta
     9181 gcatcgttta ttaaaaatat tggcaaatca gacagttctt ttataaaagc tactatattt
     9241 aggtttgata catttccaaa gtctggggga tttagtatgg taccatttat atcatctact
     9301 ggacctattg aagaaatacc aattccaatt atatctctct tgttgatttt cattattttg
     9361 gtaaacattc tttttattat atcttttaag acatcatttg atagacattt agggtaagtt
     9421 tcagaaagtt gttttacaat attacctttt aaatctgaaa taattacctt acatatattt
     9481 cttcttatta ggattccaca aatacaaggt gatatattag aaatatctaa taaaataggt
     9541 ttacgacctg atgaatgtgc atttaaagtt gatgactcta tttccttaat catgttttca
     9601 ttaataagtt cagttactag atttgataaa gtcattttag taagtcctgt agacttagct
     9661 aaatcaaccc tggacatagg tgtatttgta gaaagtaatt tcaatattat taacttattt
     9721 ttaattttta catcttttat gttagaacct tgtttgttac tcatcttaaa tctccttaaa
     9781 taaagttaag tgttttattt attttcaagt atgctatcac cttataatag agcaacattt
     9841 ttttacttga aaatatcatt aattagaata tatcacatta tatatcataa aacacaaaaa
     9901 aatgttaggc ttaaattaaa aattaaaagt taaaagttaa aagtagattg agatataaat
     9961 agtagatact caattaaaaa agataatgat aacaatatct gcttttataa agaaaccacc
    10021 atttcaagca ttactttaag tagttaagtt gatggtagtt catatataat aacttgataa
    10081 aaggtttact taataataaa tagattatgc aaatctacaa tctatttatt gtatacaata
    10141 aaatttcatt ttgtaaaaca gtgaattaaa tatgaaatac agagatagat ttttattaca
    10201 aattgttacc aaatatagat atattagaac agaaatctag taaattgtaa ctaaaatgta
    10261 aatttttcct ttaatctata caatattatg gaaacttttt gtaatctatg atataataac
    10321 aaatatataa ttattaaatt ataataaatg actatagcgt agatgtatta ctatgctata
    10381 gtcatttttg aattaaactt gatatatcaa gggggaagaa tatgagaaca attagaagag
    10441 ttttagcatt aggcttaaca ttagctatat ttctaataaa tgtacctaat gtagatgcat
    10501 taacatcaga tacaattaaa ggtaataata tatatgagac ggctggatca atagcagata
    10561 aaaagagtta tgatacagca attatggtaa atatggataa ttctatagca gatggtcttt
    10621 cagcaagtgg tcttgcaggt gctgttgatg ctccaatttt gcttgcacaa aaaaataaga
    10681 tacctagtga aacaaaacaa agactaaaaa atgttaaaaa aatatacata ataggtaaag
    10741 aattatcaat aagtaaatca gtagaaaacg aactgaaaaa tacaggagca caggtaacta
    10801 gattgggtgg agatgacaga ataaagacta gttatagtgt tgctaaagag gtaaatggta
    10861 tcaaaaaagt tgatgaagtg atactaacta atgcatataa aggagaagca gatacaataa
    10921 gtgctgcacc tgtatcagta agagatatag ctcctatagt acttacagat ggaaagagtg
    10981 tacctttttc aacaagtaat gtaaaaacat atgcagtagg tggaagtata tccatgagca
    11041 ctagtttagt taataaaact aatgctaaaa gacttggcgg ttcagataga tatgatacaa
    11101 ataagaaagt tataaaagag ttttatccag atgcatcaga attttattta agtgatggat
    11161 atgatttagt aaatgcactt acaggttcaa caattgctaa ggaaaatcca attgtattgg
    11221 tgtcagaaag tagtgataag tctatattag caggagcaga taagattact agattaggtt
    11281 caataagtga tagtgtgtat aataagtgcg tttctgttgc acaaaataat ggagattcgt
    11341 ctacaaaagg tgattcacct atgaaaaatg agacaagcat attaggtcag ccaacagcta
    11401 gtttagaagc ttgtttaaaa tgggccaaat ctaaaaaagc taatgattta tttatagagt
    11461 taataccaat attatatgat actgctgttc aggaaggtgt aaatcctgtt ttggcagtag
    11521 ctcaatctgc aaaagaaact ggtttttgta attttggtgg agtgttagat gcatcattta
    11581 agaatccttg tggacttaaa acttctgtag gaggttctga tactgataag aatgctcatt
    11641 caagatttga tacttgggaa gaaggaatat tagctcaaat tcaacattta tgcttatatg
    11701 caggacaaga tggttatcca ctttcaaatc cagtagaccc tagacatgaa aagtctttat
    11761 ttggtaaagc taagacagtt gaaagtctat ctaataattg ggctggaggt caatatggac
    11821 aagatttagt aagaatgatg ggagaaattg aggcaacaaa ataattgaag attatcaaat
    11881 atccaataaa taaggattga ttattatgaa aaagagtaat atttttagta ttatatttcc
    11941 tacagtcata atggtgctaa tgtttttaac ttttgttaca gatatattta atcttgcaga
    12001 tgttcaatct aaaagtattt ttgttgtagg aatggttttg atttttccaa tttctttttt
    12061 aatacaagga ataatttgtg tattaaataa gactagttgg attttatctc ttattgtgtc
    12121 tttattaact tatatagtat taatgtatat atttttaaat gattctgcat atatatatgt
    12181 tttactttat acagcttttt atatgattgg acatatagca gccaagttct attacaaaat
    12241 taagactttt aaaaattaaa tttaagtata taaaattaat attaagggaa gtatctttaa
    12301 tgattttcta aaatcaaaaa taggtgcttc tctttttgta aaaataaaga tatgtaaata
    12361 aaatttaacc attataattt tcttttaaca aatgtataca ataaacttgt tgtataatta
    12421 agatagataa aataaaggga gggataatat gaatttaata gataatcata ttcatactaa
    12481 tttttcaagt gatggaaagg attcaatgga agatacaata aaaaaagcta taagtattgg
    12541 tgttagatat ttaactttta cagaccattt agaacatgac gaagatagag gattttgcat
    12601 aaattataat gactatgttc cagtgtttaa caagtttaaa gaaaaatata aaaaagacat
    12661 agagttatta ttaggtgttg aagtaggtta tagaaaacat ctaaaaaatg aaatagaaga
    12721 aataataaat tctaatcctt ttgattttgt attgtgctca actcatacaa ttgacaatat
    12781 acctgttcca tcaaaggcat attttaaagg acttagcaaa gaagatgcgt attataaata
    12841 ttttaatagc atacttgaaa ctaatcgtga atttagagat tataatatat atggtcattt
    12901 agattatata tctaggtatg gaatatattc agataataga gttatataca atgactttaa
    12961 agatattatt gatgaggttt taaaatcaat aatcaataat ggtagtggta tagagctaaa
    13021 tacatctggt tatagatacg gactgaatgc tatacatcca aatgaagata ttctaaaaag
    13081 atataaagaa cttggagggt ttatagttac tgtaggttct gattctcata gagtagaaga
    13141 tatatgcaaa gattttgatg tggcctatga tatgcttaag tatcttgact ttaaatatgt
    13201 atctttattt aaagaaagag aaacttattt tataaatata gaaaaagtta aatctaacaa
    13261 tatagcttag agtttatttt gaagtaaatt aatggatagt taaaaagtca atagttataa
    13321 tattgtatcc tctagaaaaa tactagaggt ttttattttg caaaaataac taaatagaat
    13381 taaatttaaa caatatagtt tattttaaat tgaatagttt attgttgata tattttacta
    13441 ttatatattt ttattgtgat ataatagtaa aatatattaa cctatcttta gattatataa
    13501 attattataa aggagggaaa tttcaaaatg tctaataaaa ataaagataa tataacttta
    13561 agtaatcaaa tcaaaagaac aactcaaaat agtttaacaa aagaagctat attacatata
    13621 ccaatgagta attatgccta tggttatgat agagaaacac tacatataag acttagaact
    13681 aaaaaaaatg aagctaaaag agtaatttta cgaataggag accaatatgt ttgggataaa
    13741 ggtggtgcag gaggaggaaa tttaaatgca tctggagttg gttggtctgg tggaacaaat
    13801 ataataatga acaaagaagt agaaactgaa ttatttgatt attggatagc taagtgtaaa
    13861 cctttaaata agcgttcacg atatggcttt ataatagaag gccaaaaaga aaagatttta
    13921 tttacagaaa aaagaataat agaattaggg aatgaaaatg atgaaaaaga attatgtgaa
    13981 ataagtaatt tttttggata tccatattta aattatatag atatacctaa agtgccaaat
    14041 tgggtgaaag atactgtttg gtatcagata tttccagata gatttgcaaa tggagattca
    14101 tctataaatc cagaaggaac agatgaatgg ggagctactc caactagaga aaattttaca
    14161 ggtggagatt tacaaggtgt gatagagcac ttagattatt tgagtgattt ggggataagt
    14221 ggactttact tttgccctat tacaattgga aaaaccaatc atagatacga tacaatagat
    14281 tatatggaga tagaccctgc cttaggggat aaaaggactt taaaaaaact aatagaagaa
    14341 gcacataaga gaaatataaa aataatgttg gatgctgttt ttaatcatat aggatattat
    14401 tctaaacagt ggcaagatgt agttcaaaat aaggaaaatt ctcgatataa agactggttt
    14461 tatataaaag atatgaataa aattgatatt ccaatagaac agatagatga aaaaaatata
    14521 ccttatgaaa cctttgggtg tgaaaaatat atgcctaaac ttaatacaga aaattcagaa
    14581 gtaatagaat atttattgga tgtaggtaag tactggatac atgaatttga tatagatgca
    14641 tggagattag atgtttcaaa tgaagtagac catgcttttt ggagaaagtt cagagaagaa
    14701 gttaaaaaag taaaaccaga catctatata ttgggagaaa tttggcatgg tagtttacca
    14761 tggctaatgg gtgaccaatt tgattcagtt atgaattact tgatgtcaga agccatgaaa
    14821 aaattctttt gtacagatga gataaatgct gaagagttta agtatatgat aaatgatgta
    14881 atggtaagtt atccaataca agttaatgaa gttattttta atttacttgg aagtcatgat
    14941 actacaagaa tattgactta tgctaatgga aatgttgata gatttaaact tgcatatatg
    15001 tttatgtttg tacaaacggg atgtccttgt atatactatg gtgatgaaat tggaatggaa
    15061 ggagaattaa gttcaatatc tgaaggacaa agaaaatgta tggaatggaa tgaagaaaaa
    15121 tggaatagag atatatttaa ttttatgaag aagattatta ggcttcgtaa agaaaataaa
    15181 gagcttaggt ctatatcaaa tgaatgggtg ttagcagata aggaaaatgg agcaattata
    15241 ttaaaaaaag agtccatttt tataataata aataattcat ctaaagatat aattttgaca
    15301 ttaccagatt atttagcaaa taaaaaagta aaagatttat atgaagaaga aatagttgat
    15361 ttaaaagaag aactaatgtt agagaaatac aagttcatta ttttaaaata atacaaaagg
    15421 gtatttattt tagattttat gttaaattat tatacagttt aaacaaatct ttaagaagat
    15481 aaaaaaatat ggtctataag ggaatgaatt agaagtagat tttaaattaa agactagatt
    15541 agaatagata attttataaa taatatctta aatacaagaa agaaaataaa ttgattatta
    15601 gtctaaaaaa ttcttgtaga aagagggaat aagaatgatt gaagcattta tatttgatta
    15661 aaaaatagct acacacactt gtgactttag tc
//
LOCUS       DAMJVM010000082        15588 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.086, whole genome shotgun sequence.
ACCESSION   DAMJVM010000082 DAMJVM010000000
VERSION     DAMJVM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15588
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.086"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            78..1139
                     /gene="ytvI"
                     /locus_tag="PC523_002689"
     CDS             78..1139
                     /gene="ytvI"
                     /locus_tag="PC523_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HDF2476196.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIKLITNNTNLNRTLISIVILLAFYGLCIFLLISFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALMLGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            complement(1294..2547)
                     /locus_tag="PC523_002690"
     CDS             complement(1294..2547)
                     /locus_tag="PC523_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HDF2476197.1"
                     /translation="MDLSSIFKHYCKECENTWNNSSVELFDNIETYSKDSQKKMEKEL
                     DKLINTISVHLERYPSDIVLRKMWVKKGEIFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDSDLPISDIMQAMRNVWISNALQVLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTSIDKNDKILFNNWLEKRLLGEHIKSNDYHESKVSQMIDYIESVYPREKFIEV
                     YESLLLIFKSQVKSLKQHGEENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEID
                     FCIGYGFLLQISDDLQDVKEDLKYNHKTIITEMAKEGNLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVERFIPYTIDYSLEIEEKMK
                     EKFKSIDVLNNENEYKEMIDIICAE"
     gene            complement(2648..4768)
                     /locus_tag="PC523_002691"
     CDS             complement(2648..4768)
                     /locus_tag="PC523_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDF2476198.1"
                     /translation="MGKVLVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVVTWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKEGFAKLKSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSRICNKLLKSNITANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKVTSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPIDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQKKNQETFNNPFAEALAKLKNK"
     regulatory      5195..5283
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            5473..6840
                     /locus_tag="PC523_002692"
     CDS             5473..6840
                     /locus_tag="PC523_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDF2476199.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILVSFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     gene            complement(7013..7660)
                     /locus_tag="PC523_002693"
     CDS             complement(7013..7660)
                     /locus_tag="PC523_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDF2476200.1"
                     /translation="MLESLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKNTGYILITPS
                     GIGREELKVEHICVLDLDGNVIEFEKGIKPSSEVLMHIEIYKTRENVNAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKIVSKLLLDTDACLLERHGL
                     TTVGNDLAEALLKSRYVEDVAEIYYKSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(7678..8364)
                     /gene="araD"
                     /locus_tag="PC523_002694"
     CDS             complement(7678..8364)
                     /gene="araD"
                     /locus_tag="PC523_002694"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDF2476201.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNVVDGKLRPSSDTPTHLVLYREFEELGGIVHTHSSWATI
                     WAQIGKPIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNLMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            complement(8461..9519)
                     /locus_tag="PC523_002695"
     CDS             complement(8461..9519)
                     /locus_tag="PC523_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2476202.1"
                     /translation="MGNKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGECDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEKHVLKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIKAGDSVVVLGCGPIGQFSIQWARVFGASKVIAVDIFDEKLELSKFLGANYILNS
                     KEVNVIKEIKTITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATERILRGELTLKGSWNSYTSPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            complement(9864..11270)
                     /locus_tag="PC523_002696"
     CDS             complement(9864..11270)
                     /locus_tag="PC523_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2476203.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRITTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGIAVGIYFALWALFRKNKASIL
                     DYLEKQALKNVEEEPVAV"
     gene            complement(11449..11751)
                     /locus_tag="PC523_002697"
     CDS             complement(11449..11751)
                     /locus_tag="PC523_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2476204.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            complement(11872..12318)
                     /locus_tag="PC523_002698"
     CDS             complement(11872..12318)
                     /locus_tag="PC523_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2476205.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKDTYINAVLERE
                     KTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLNVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNVKLLKNIKNASTKEEVAKLLDFIEI"
     gene            complement(12351..15254)
                     /locus_tag="PC523_002699"
     CDS             complement(12351..15254)
                     /locus_tag="PC523_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2476206.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISTILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     EVVEETEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIVGESGVGKSLFS
                     RHMYEFAKQKKIIKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDAPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     VEYTEIRKYLKNAIFIPFNLEEALVKGNLLSDKYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSSEDIFSMSDLNSFVNDSLVKLVKELMNDIVEKNI
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKIPDDEIGFITLYIKSALKNEAKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNINTKTID
                     RVDLLMALEAARKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIVTLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENIKETSNILAIVGTINPKVPGINFI
                     PYNKEVFKNLDIYLSKSKQQDDRPIKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGFIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDEEFMNNANKCFNKDAFKKIIFDKFKHL"
BASE COUNT         4940 a         2691 c         1775 g         6182 t
ORIGIN      
        1 atcaatatac agtagtatat tttatgtaaa aaagaccata tttagaataa tattaaatac
       61 actggaggtt ttatattatg gacattcaaa agcaaaagaa ttttataatt aaatttacat
      121 atgtagtttt aataacaagt attgtatata ttgtattaaa attcttattg ccattactaa
      181 tgccttttgt aataagtttt atcattgcat ccatcctaag acctattata aaacttatta
      241 caaataatac aaatttaaat cgtacactta tttctatagt aatactgtta gctttttatg
      301 ggttgtgtat attcttatta attagctttg gtgtgaagat atttgcaagt gtaagtgatg
      361 tattttttag acttcccgaa atatataaat caagtataca accaacacta aatactttat
      421 tttcgaagat agatgctagt acacctaatg ttaacctagc attaatgctt ggttgggata
      481 atataagtca atctatgatg tctttagtag cttctatttc aacaaatgca cttaatgcta
      541 ttgctagtat tgcaagtaaa acacctgcat ttatgttaaa acttattatt actttaattg
      601 cttctttttt ctttactttt gactatcaaa aaatagtaaa ctttatctta aaacaatttc
      661 cagaaaaaag tcaatttatg ataataaata taaaaaatag cagtattaat gctcttttaa
      721 aactactaaa agcttacgca atactactat cagtgacatt catagaacta cttattggac
      781 ttactatcct aaaagttgaa aatgcattta ctatatctgt catcatagca cttgttgata
      841 ttttgccagt cttgggtaca ggaagtattc tgacaccttg gatgattata tcactcatta
      901 atggaaatat taatttagca ataggacttt taatactata cataattata actgttgtta
      961 gacaaatatt agaaccaaag gtagtgggac accaaattgg cctatatcct ttaataacat
     1021 taatgtgtat gtttgttgga gctcaactat ttggaatagc tggattattt ggattcccta
     1081 tcgcagctac aatcataaaa aacttacatg ataatgggat tattactgca tttaaatagt
     1141 ttaaccttga catttttatt taaattaaaa agggctgtct caaatagaga taagcctctt
     1201 tttaattgtt agaatgatga aaatacattt gattttatac aaaaagagtg ccttataaac
     1261 taaaaagtct attctaagac actccctttt tttctactct gcacaaataa tatcaatcat
     1321 ttctttatac tcgttttcat tatttaaaac atctatactt ttaaattttt ctttcatttt
     1381 ttcttcaatc tctaatgaat agtctattgt atatggaata aatctctcta cttcttttat
     1441 atatcctaca gaaaaaaatt cagcattata aactacagaa aataaaatta acattaagca
     1501 atcattcttt atcatagtta ttacagattt atttttattg ttaattttaa aactatctat
     1561 taactcaata gtaaaattta ttagtttatt tacaacttta tctaaattac cctcttttgc
     1621 catctctgta ataatagttt tatggttgta ttttaaatct tcttttacat cttgtaaatc
     1681 atcagatatt tgtaataaaa atccatagcc gatacaaaaa tctatttctt cctttgtcat
     1741 caatccactt attaaatatc catctactaa aacggatgaa ccaccttttt caatagaaat
     1801 ggataataaa tcttccttac acaaatgatt ttcttcgcca tgttgtttta aactctttac
     1861 ttgactttta aatattaata ataatgattc atatacttct ataaactttt ctctagggta
     1921 tacactttca atataatcta tcatttgaga tactttgctc tcatgatagt cattagattt
     1981 aatgtgttct cccaagagcc ttttttctaa ccagttatta aataaaatct tatcattttt
     2041 atctatactt gtattgtcta aataattatc agtatatgga tataacatac tatatgcaaa
     2101 gttagctttt gaataataaa cttctttacc aaataatact tgtaatgcat ttgaaatcca
     2161 tacattcctc atagcttgca taatatcact tataggtaaa tcactatcaa attttctagc
     2221 atctctaata aattgttttg ttatatctaa aaattttttt ctatcctcta catccatttt
     2281 ttctaactta aaaatatttt ctttttctaa tgtcttttgt aagaaaatct cgcctttttt
     2341 tacccacatt tttctcaata caatatcact tggatacctc tctaaatgaa ctgatattgt
     2401 atttatcaat ttatctaatt ctttttccat ctttttttgt gaatctttac tatatgtttc
     2461 tatattatca aatagttcaa cacttgaatt attccatgta ttttcacact ctttacaata
     2521 gtgtttaaat atactactta aatccattta acagccctac tttcatgcct aaactataca
     2581 ctatattata tcagtacata taactaatat acagttaaag ttataattgt gtataataac
     2641 tttaaaatta tttgtttttt agcttagcta atgcttctgc aaatggatta ttaaatgttt
     2701 cttgattttt cttttgattt tttaaatatt gattaacatc tttttttgat gccttctgct
     2761 tttcttcttg ctttctcttg ttaaaagaag atagcttttc tctgtatccg caactacaag
     2821 tgaagattct cccttctcct tctccacgaa gttctaattt tttatgacaa ttagggcatc
     2881 ttgaattggt tacttgtgat atagtttttc tagtattaca ctctctgtct tcacaaatca
     2941 acatcttacc ttttttacca tttacctcta gcataaattt accacactct ggacatctat
     3001 ttttagtcaa gttatcatgt ttaaatttat tatcactatt ttttacttct aatacaatat
     3061 cttttgaata atctttcata tcatttatga atgtattttt actaagtttt ccctttgaaa
     3121 tatccaataa tttcttttcc catgaagctg taagttctgg tgtcttcaag tctattggaa
     3181 ctaaatctaa taattgtctc ccttttgatg ttatatatat atctttattt ttcttttcca
     3241 ttaaaaatga attgaataat ttttctatta tatctgctct tgtagcaact gtccctagac
     3301 cgtttttttc catagcagtc agtaatgttc cctcatttaa atatggtggt ggatttgttt
     3361 ttcctgacgt tacctttaat cccttaacct taagtacatc tttttctaaa acatttggta
     3421 tactttcata ctcatcttcg tcttcaatat ctttataact ttctttccaa cctaaattta
     3481 ttactttgtt tcctttagcc ttaaatatct ctccttctac agttgcactt atagttgtct
     3541 gctcatattc aaaaggagga gatagtactg ctaaaaatct ttttactaca agattatata
     3601 tttttcgctc tttgtcacta aaatcactca taaatacctt ctgttctgtt ggtattattg
     3661 catggtgatc tgttaccttt gaattgtcaa caaatgattt attagctgta atattgcttt
     3721 ttaataattt attacaaatc cttgaataat cactaatatt tacagctttt attctatctt
     3781 ttaatgttcc aacaatatct gatgttaaat atctagaatc agttctagga taagtaagca
     3841 ctttatgatg ctcatatagc ttttgcatta tagataaagt ttcttttgct gaaaatccaa
     3901 acattttatt tgcatctctt tgtaattctg ttaaatcata gagtgcaggt gaatattttt
     3961 tcttatatga tttgtttact tcagatattt ttatgtctac accttttact ttttttaatg
     4021 tagattctat cttttcttta ctaaaagttg aagagttatt gtttttatca ttccaaataa
     4081 attttatatc tgaattgcct tttgtagcaa ttaattccaa tccatagtac tccttgggtt
     4141 tgaaatttct tatttcctct tctcttttaa gtaacattgc aagagtagga gtttgtactc
     4201 ttccacaaga aagttgtgca ttatactttg ttgtcaaagc tctggttgca tttattccaa
     4261 taatccaatc agcttctgct cgcgctattg ccgaataata caaattttca tattcttttc
     4321 cactttttaa ttttgcaaag ccttctttta ttgctttatc tgtacttgaa gaaatccaaa
     4381 gacgctttat aggttttttt acctgtgatt tttctattat ccatcttgct acaagttctc
     4441 cttcacgacc tgcatctgtt gctataacta tttcatctat atcatttcta ttcatttgcg
     4501 atttgactgt attaaactgc tttccactct tttttatcac aacagtctta aggtgcttag
     4561 gaagtattgg caagtcttct aaactccacg atttgtatct ttctccatag ctttcggggt
     4621 cagcaagtgt aaccaaatga cctagtgccc aagttaccac atactttggt ccctctatat
     4681 aaccattttt ttcatttttt gaccctaaaa cttttgctat atctcttcca acagaaggtt
     4741 tttctgctag tactaatact tttcccatct aaaaatacat cctttctcta tacataatct
     4801 catattacgc ttaaaatcta agtaaataga acttttctta atatcgtaac tataatatta
     4861 ccataattta atcatatcta attaattatt taagatttta ttgttttttt atttaaaact
     4921 tattttacac ctaatttgta tgtctattac aaaatttatt ttcacgtata ttactcatct
     4981 ttctaagttt ataattcgta aataggctta catttttatc ttttttcttt gttttttaat
     5041 ttttcagttg acaaaacata aaaacttata ttaatattat taatacaata agctatgaca
     5101 aatcaatatc cggttaagat tttatagaag agttgctata tggcacaccg aaggagtaac
     5161 actctcaggt acttttaaag taggactgta aaatttacgg aactctggag agacccatta
     5221 tgggcgccga aggggcaagg cttttatgct caatctctca ggcaaaagga cagggattat
     5281 ctaccaaaaa cagtatttac taatactttt attttggttg ctaattagtg tacatccaga
     5341 gctgaatctt ttttagattt agcttttttt attacactag aaattattaa taaattatac
     5401 ttatttcctg tcaattactt cattaaaaat tctattgatt atgtaaagca aaaaaaatga
     5461 gggagtgttt ttatgaatct aattgatatt ttaaacaaag ttgatgcttt tatttgggga
     5521 ccacccctac tagtactatt agttggtact ggtatactac ttactgttaa acttggagtt
     5581 gttcaaataa ctaaactacc aagagctctt aagttaatat tctctgctga aaacaaaggt
     5641 agtggtgatg tttcaagttt tgcagcctta tgtactgctt tagctgctac agttggtact
     5701 ggtaatattg ttggtgttgc tacagctatc aaagctggtg gacctggtgc acttttttgg
     5761 atgtggatag cagcattctt tggaatggct actaagtatt cagaaggtgt tcttgcaata
     5821 aaatacagaa ctaaagataa aaatggtcaa gtgtctggtg gaccaatgta ttacatagta
     5881 aatggtatgg gagaaaaatg gagacctctt gcaatatttt tcgctataag tggtatacta
     5941 gttgcacttt taggaatagg tacttttact caagtaaatt caataacaga cgcaataaat
     6001 aatagttttg gtatagaccc tagaattaca ggggttgtat tagcagtatt tgtagctttg
     6061 gttgtatttg gaggtcttaa aagtatatct aacgtagcta ctaaaatagt accttttatg
     6121 gcagttatat acgttgtaat ctgtggaatc attttagtat ctttttggaa taaaatccct
     6181 gaaactttta tgttaataat taaaagtgca tttacaccta ctgctgctac tggtggtttt
     6241 ctaggagcaa ctatgtcctt agcaattaga aatggtatcg ctcgtggtgt attttcaaat
     6301 gagtctggtc taggtagtgc tccaatagct gcagctgctg ctaaaactga atggccagcg
     6361 gaacaaggtc ttatatctat gacaggtact tttatagata ctattataat ctgtactctt
     6421 actggattct ctcttgttat ttcaggggta tggtgttcag acctaaatgg agctgttatg
     6481 acacaagcag cttttaacgg ggctattcct acatttggac caatattact tacagttagt
     6541 ttaacattat ttgcatttac tacaatttta ggatggagtt attatggaga aagatgtttt
     6601 gaatttctgt ttggtgtaaa aggtatgaat gggtatagaa ctgtatttgt ggctatggtg
     6661 ttattaggtg cattcttaaa gttagaagtt gtttggataa ttgctgatat cgtaaatgga
     6721 cttatggcaa tacctaactt gatagcttta cttgcattat cacctataat agtatctgaa
     6781 actaaaaaat attttgaaca tataaattca ccagaaaatc agataaagaa aaatgcataa
     6841 ataaaaaata tagaatccca agtcaatttt atatggcttt cggattctat atttttttga
     6901 aaaaatgtaa taaatatatt tacaaaagac gataattaac ataatacaat atagtaattg
     6961 atataaataa tacatatatt ttaattaacc acttcaataa aaattactta gattaaaact
     7021 ttatttcttc tgggtatttc caagatttaa actcatcaac atctactact tcaagttctt
     7081 taaattgatt taatactaaa gacttatagt atatttctgc tacatcttcc acatatcttg
     7141 attttaataa tgcttcggct aaatcattac ctacagttgt caacccatgt ctttctaata
     7201 agcatgcatc tgtatctaac agtaattttg aaacgatctt agctaatttt aaagttcctg
     7261 gtctttcgta tggagcaata tctacataac cgccataatt aactgattca tatacaattg
     7321 gtataattgg tttctttgcc actgaaaatg ctgttgcata taaagaatga gtatgcgata
     7381 tagcatttac attttccctt gttttatata tttcaatatg catcaatacc tcactgcttg
     7441 gcttaatacc tttctcaaat tctattacat ttccatccaa atccaataca caaatatgtt
     7501 caacttttaa ttcttctctt cctatacctg atggagttat aagtatatat ccagtattct
     7561 tatctctaat actaaaatta ccagtctttt ctttacataa accataatgc tctgctttaa
     7621 ttgctatttc cacaacttta ccttttaaag actctagcat aacttacctc cagacaatca
     7681 tttttgacca taatatgcat tttttccatg ttttcttgaa aaatgcttat tcataagaac
     7741 cttatccata agattatcag gactcattag ctctgtataa taagccatct ttgcgacttc
     7801 ttctaaaact actgcattgt gtacagcctc atttgcatct tttccccatg taaaaggtcc
     7861 gtggtcattt acaattattc ctggacaatg aattggattt cttcccataa attcttcaat
     7921 aattacattt ccagtttcct tttcatattc attgcaaatt tcttctctag tcatttttct
     7981 ggtacatggt atacttccat aaaaataatc tgcatgagta gtaccacaag atggtatagg
     8041 ttttcctatt tgagcccaaa ttgttgccca acttgaatgc gtatgtacta ttcctcccaa
     8101 ttcttcaaac tctctatata aaactagatg agttggagta tctgaagaag gtcttagttt
     8161 gccatcaact acatttccct ctaaatcaac tacaaccata tcttctacag tcatattact
     8221 atactctaca ccacttggtt ttatcactac caatcctttt tctctatcta tgccacttac
     8281 atttccccaa gtataagtaa ttagcttttt tttaggtagt tctaaatttg cttttaatac
     8341 ttcttctttt aaattttcta acacttttct tcctcctata ttttatttta aaaaattatt
     8401 tctattttgt ttaatgtcat aattatgaaa tttaattgtt ttagaatttt tattaaattt
     8461 ttatatttct actaggattt tattgaaatt aatctctctc ttgctcattt tacttaaaaa
     8521 gtcaccaacc tcattaagac ctattttatg tgaaatcata ggtttaaaaa taatatctcc
     8581 ttttcccata aaatccaatg ttgcagtcca tgctcttcca ggataagggc ttgtatatga
     8641 attccaagag cctttcaaag taagttcacc acgtaatata cgctcagtag cgtctgcact
     8701 aagtggtaat tctgtgtgtg atatacccac aaatactata tttcctctct tttttgctac
     8761 aaatagagat tgttcttgtg taaatcggct accagcagtc tctattacaa catcagcacc
     8821 accatttgtt attgttttta tttctttaat tacatttact tcttttgagt tcagtatata
     8881 gtttgctcct agaaatttag aaagttctaa cttttcatca aaaatatcaa ctgctatgac
     8941 cttagatgca ccaaataccc ttgcccattg tatagaaaac tgacctattg gaccacatcc
     9001 taatacaacc acactatctc cagctttaat attagacttt gaaatcccat gataagctat
     9061 agttgctggt tcaattccag cagcagtttc ataatccagt gtatctggta gtttcaagac
     9121 atgtttttct ggaactctta catactctgc aaatgctcca tttactcttg ttccaattat
     9181 attgtaatca tcacacaagc caaaattacc ttcattacaa taatcacatt ctccacaagg
     9241 tactaatggt gcaacggcta ctctatctcc tacattaata tgctttactt tttctccaat
     9301 tttgacaact tcaccagaaa attcatgtcc taaaatcaat ggatattttg tatttccact
     9361 caaaccactt accatagacc taggtaaatc tgaaccacat attccaacat attttacatt
     9421 tacaagcact tgtgtatctg ttatttctgg tatatctacc atttcatatc taacatcacc
     9481 tacattatac aatactgatg ctctcatttt atttcccata taaatatctc ctttcatgct
     9541 atatatttct atatagcaat atctatgcca tacaaaagat aaatggttta ttcaggtaaa
     9601 taccttatat acaaagtctt ttgccaatag gaattacatt ttataaaatc aaattacatt
     9661 actgtataaa tataaaagag tatgttttaa atgacaggtt atacagaaaa ctcaactata
     9721 aaaatacaat gctttacaat tacaaatatt acctaaataa aaagaagtct atacttcatt
     9781 taaatatttc tcaggtacta tctgatactt atataaaatc aattataaac tcctttaatt
     9841 attcctattt catttttata aacttaaact gctacaggtt cttcttctac atttttaagt
     9901 gcttgttttt ctagataatc aagaatactt gctttgttct ttctaaataa tgcccataaa
     9961 gcaaagtaaa ttccaactgc aatacctata taaattggat ttgcacttag aaatgcaaag
    10021 aaaactaatc ccatcaaagg ttttcctagt atattaaaac ttgttattaa cattgctcct
    10081 gcaggtatag ctacacctat atttgtagcc atatctgtaa ataatggtgc tgtagatgta
    10141 caaacatata atcctaatcc aaaccaaatt atacctgaaa ctagtacttt tggtatatta
    10201 ccattatgta tggcaactaa accttgaacc atatatggaa ttgctaataa atccacaact
    10261 ggtaatactt tattgccagg aagtaccata gctattacaa gcattattgg aattaatata
    10321 agacctgata ttaaagttgc aggttcacca tacccaacag catcatttac tgctatgtac
    10381 cactcacggt ttccagcttt ttgcataatc tttctagctg cttctgttat aggtgcaaat
    10441 gcttgagcaa acatgcttgc tacttttggg aatattgcca taactgcact tgtagatata
    10501 cctactttca ttatttctcc ccaagcttcc atagtagtta ttcttgtcat attacctaat
    10561 atacctataa acatacctaa aaaaagacct aaagtaattg gttcacctaa gaatcctaat
    10621 ttcttctcta attcttttgg atttaatttt attttattta atcccaattt attatatata
    10681 gggtctgcaa atactgcaaa cacagaagcc tcaacattat gcatagctat tatcgtacaa
    10741 ttaggatatc tatagtaact agaccatctc tttgcaacta actctgatat taacaaacta
    10801 tataagttta ataaaaccat acatgctata cctaaaggaa aatttcctgt tactcctata
    10861 accattgctc cccataccat gtaagaatag ttattccata aatccgatgg ttgaaacacg
    10921 tctgtccatt ttatcaaaaa taaaattgtt tgtaataata tcccaagacc caaatatatc
    10981 atacctgcac ttgttgaaaa tgcaaccaat gatgttgctt gccagccaac atcaaaaact
    11041 ggtaaattta ctcctgtact ctcaaccatc ctgttgataa ctggtgttat tattggtgta
    11101 aatgaattta aaatcaatgt aaaaccttgt aaagcaactc ctgcgtacac agcagaaaga
    11161 aacgatttct tagtagttac tttaaatatc tttgctatta tgaatattat aattggaact
    11221 ataattgcag aaccaaatgt atcgattata gatttcataa attctaacat ctttttcctc
    11281 ctatttataa atatttataa gcttagttta atatctaaat gactattttt ataatttttt
    11341 aatttatgac aacatataaa atgttttttt acaaatattt agttaatatt agcttattat
    11401 agtatctaaa tcattgattt aaatactata aattcacatc tccaaaactt acttatctat
    11461 cccttcaata acagtcaaaa cttcttcaaa gaatgcctct tcccctatac cagttaggca
    11521 agcaaatgca tttatagttg gtattccata atcgcctgtt tgaagtggac tagtaaatgt
    11581 tataaaatca aatcttcctg attgcgctaa atttagtgct tctgttggtc tagcttctgt
    11641 tgtagtaata gaaattccct tattccctaa tacttctttt aatttacctg ctaccataga
    11701 tgatgtaact gtccctgaac cacaaactga taatacatta aattgtttca taataatttc
    11761 ctcctaattt ttcactctaa atttttattt tatatatatt aaacctatta aaaaataaca
    11821 cttactttga ttgtttaaat tttaattttt tatttataaa attctcatac attatatttc
    11881 tataaagtcc aataatttag ctacttcttc ctttgttgat gcgtttttta tattttttaa
    11941 taactttaca ttttgaaaaa ctgacattaa atcttttaac agttttactt gtgaatctgg
    12001 gttctttact gccaataaaa atacaagttc tacattaagc ctatcttttg ggtctatcat
    12061 tgaattaaat tttacaggat ttttcaatat ccctacagct acattagatt catttacatg
    12121 ctttgaatct gtatgtggaa tagcaacaca catttctcca atatcgagac ctgttggtaa
    12181 tgtcttttct ctttccaaga cagcatttat gtatgtgtct tttacatatt cttttttaca
    12241 cattatactt cctaattctt caataacact ttcataatca ttagattcta aacctaatga
    12301 tattaaatct acattcatta ctctgactcc tctcaatttt tttgctatgg ctacagatgt
    12361 ttaaatttat caaatataat ctttttaaaa gcatctttat taaagcactt attggcattg
    12421 ttcataaatt cttcatcttt tagtatctca agaacattct taacaatctc tttatcatta
    12481 gcctgaaaaa ctggcataaa tatgaaattt acattgccta caccccaatc aatagggttc
    12541 tttagtttga caaaaacaaa cctggtacta tttacagtag aagaatctcc atgaggcaca
    12601 cctataaatc ctttagaata tgttggaagc atctcctctc tatgtaaaac agaatctaaa
    12661 taatcttttt ctacatattc tttatttata agaattgaac atacatattc taataaatcc
    12721 tttttaaaat atatatctgg ttcaaaaatc actaaatcct catcaagcat tctttcatac
    12781 gatttaattg gtctgtcatc ttgttgtttt gattttgata gatatatatc caaatttttg
    12841 aaaacttctt tattgtaggg tataaaattt atacctggta ccttaggatt tattgtccca
    12901 acaatagcca atatattgct tgtctccttt atattttcaa ttttacaaaa tagattttca
    12961 tccaatgcac tcatttgata gcatttagtg ttatcatact tttcttccaa tgttttactt
    13021 atatatttag cagttccttc tccagtaaga cataatgtta caattgcatt tgatttatct
    13081 atgtgctttt ctaattcata gttatatccc atcctatctt ttagtaaaga aaaaaatatt
    13141 tcatcaagtt cttcctctcc catagaaact tttctagctg cttctagtgc cattaagagg
    13201 tctactctat caatagtttt agtatttata ttggttctct tatttactat ttgccctatg
    13261 tttgtaagtg agccaatatc taccaaaaac aatattcctt ttccttggtc tattattttt
    13321 gacaattcta ctgccttatt gtatatattt attggttttt catctaaagg catatctata
    13381 gccacaggaa actttacacc taaaagttct ttaactacat ttacagtctc tgtagctata
    13441 cgtccatgag aaattattat taaccccact ttgtcttttt tcgcttcatt ttttagtgca
    13501 gactttatgt agagcgttat aaatccaatt tcatcatctg gaatcttaat acctattgat
    13561 tcctcaattt ttattgcaaa acttttacta atttcatatt cttttgaaaa atcaatcttt
    13621 attttttcta aatttacgtt tattatcttt tgatttagct taattctttt tatagcttct
    13681 tctaagtgta ttgctagata tttgaacata tttttattta cttctaaatt aatatttttt
    13741 tcaactatat cattcataag ttcttttacc agtttgacta aactatcatt tacaaaacta
    13801 tttaaatcac tcattgaaaa aatatcttca ctactatcga gattaaactt taagtcagca
    13861 aatctattta aaataaaagt ccataaaata ttctctatat ctatatcttc aatatctagg
    13921 gattttaggt cataatattt cttttcaatt gttttgtata tgtcctctgg taatatatat
    13981 ttatcactta aaagatttcc ttttactaat gcttcttcta agttaaatgg tataaatata
    14041 gcatttttca agtattttct tatctctgta tattcaacct cttgaaaggc agcatcttta
    14101 aaagaatctt taagctttaa aacttcattt atatctacta ccactatcga ttcactatct
    14161 tcatctttag aatttataaa cttcataaaa gctcttgcac aaagaacctg aatcatattt
    14221 tgtaattgtc ctatatttcc actaaacttt tttagtgcaa gtatttcaat cacattttta
    14281 tctacaaata tttttgcgtt tattctatta cactcttgtt gaaagatatt atatataagg
    14341 tctattttct cttttagtaa tctatcatgt aatggtggca aagttattag cattggtatt
    14401 cttcttctga atgttaataa taagttagtc tctacattct cagtagttgc cccaatcaac
    14461 ataatactta cttttctttc agcattagtt tcacctaatc tcctaaactt tcccctatct
    14521 aagatggaaa atagtatttc ttgacctttt ggaggcaatc tatgtatttc atccaaaaat
    14581 aaaactccct catcagcttc ttctacaaga cctggtgcat catattcagc tcctgtaaat
    14641 gcaccttttt tatatccaaa taataaagac aataataatt gtggattatc actatagtca
    14701 gcacaattaa atacaacaaa attggaactt tcttttatta tctttttttg ctttgcaaac
    14761 tcatacatat gtctagaaaa taaactcttg ccaactccac tttctccaac aatcaacgtc
    14821 ggtaaccctt taggtgggta caatatagca gatttagcct gttctacagc atttctaaga
    14881 ctccctttat tacctatcag agtttcaaat gggtcattcg attcttcttc agtttcctct
    14941 actacctcca tttctaaact ttctttaggt aaaagttcct ctacaactct tctagatata
    15001 ttaaaacctt ctgtcatcaa ttctcttgta atttctgata tagaaatatt cttattcttt
    15061 tgtaatattg tcgaaattgc tgattttaga taaggatatc ttctttgcct tgaattactt
    15121 atattaagtt cttttctcaa attagttata ttctctctag ttgtacttaa aaactttgct
    15181 atttgaacat ctgttaatgg atctttttta ttttctgtat ttatgatgtt tacaagttta
    15241 tctctcaact ccatgcttaa taactccttt cttgttaaat atataagcaa ctatcatgcc
    15301 atacttactt tgatattttt ttctactcaa accctgtaag taactagatg ccataagtat
    15361 ttatacattt tgataagcaa ttacattttg tatatttagt aatttactaa atgtattagt
    15421 cattcataat cttaaatagt ttaattcttt gtttaaattg caatgcaaaa tactttataa
    15481 atatattatt gaaaaactta ctcaatcata tataatagta cgtatctact caacaataaa
    15541 aattacaata aactatgttt ttaactcttt ctgttttaaa attttatt
//
LOCUS       DAMJVM010000083        15565 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.087, whole genome shotgun sequence.
ACCESSION   DAMJVM010000083 DAMJVM010000000
VERSION     DAMJVM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15565
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.087"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(91..780)
                     /locus_tag="PC523_002700"
     CDS             complement(91..780)
                     /locus_tag="PC523_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDF2476207.1"
                     /translation="MYNVLHTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIIGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIAGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGIESITVVAIIFTGIF
                     GGMIASTVFKLGNINHPVAKGIALGTSAHALGTTKAFEFGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            complement(773..1144)
                     /locus_tag="PC523_002701"
     CDS             complement(773..1144)
                     /locus_tag="PC523_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDF2476208.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            complement(1326..2672)
                     /locus_tag="PC523_002702"
     CDS             complement(1326..2672)
                     /locus_tag="PC523_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2476209.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKAFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDISKNEETKKVKRKKNILPYIIVGCVLIGMGLYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPERKITVADEEDLYYQAL
                     DLKKKKEYEKAIENFKSIVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            complement(2854..3447)
                     /locus_tag="PC523_002703"
     CDS             complement(2854..3447)
                     /locus_tag="PC523_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HDF2476210.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            complement(3512..4966)
                     /locus_tag="PC523_002704"
     CDS             complement(3512..4966)
                     /locus_tag="PC523_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDF2476211.1"
                     /translation="MSNMNKKLVIAIGAVALVAIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVEFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SGFFGNLSKTISANFGKKSNLVVVVNYDKNKLKAEMGKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDVSKSMETFDNQTKKGNYKNKLAVKATPAKVKKEQLANIDTNLGKY
                     STTFKTSQINRSINIKLATDSISNILLMPGETFSFNEHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIANVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPS
                     TDENKNNQNTNNQQKPETPPTDNKPNETPPQPQD"
     gene            complement(5081..6472)
                     /locus_tag="PC523_002705"
     CDS             complement(5081..6472)
                     /locus_tag="PC523_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDF2476212.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIVLMIVFFLSSNE
                     FSVGKEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELKKISIDVDDLLAQADRVREMYKEENTTYDIAINYIN
                     TVSLLNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKVYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKEDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNALDEKE
                     NKYKKIEILDIEKADRYSKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAVSENGLNEY"
     gene            complement(6638..6886)
                     /locus_tag="PC523_002706"
     CDS             complement(6638..6886)
                     /locus_tag="PC523_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476213.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTFGIVL
                     CNARKIFDSKTIHKTRMKNVSTNKKQKSHSRDIKRKIS"
     gene            7152..9281
                     /locus_tag="PC523_002707"
     CDS             7152..9281
                     /locus_tag="PC523_002707"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDF2476214.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGSIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNAEE
                     VNKKIDNTCSWKKEHTKNIENECNCEHEHHDYMNKALDCKQEHMTDIKDDCNQEKKHT
                     KNTNKTHSSKQNKFKDKSCDEMKFNYDKDESCDKINSSYDKDDSSYEYFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKAEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGIVSRPQDRENFTLLLTAIR
                     DVIGDEKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNARKHQSN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPFLVKDGEFAMAYDDSLSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            complement(9494..10093)
                     /locus_tag="PC523_002708"
     CDS             complement(9494..10093)
                     /locus_tag="PC523_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HDF2476215.1"
                     /translation="MAKQKFYAVKKGKNIGIYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIYGAEKFSFKEDKEFIEAYVDGSYEHSIKMYGSGVVILKNNEVIKTYSEKGKEE
                     TLVGMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(10110..13229)
                     /locus_tag="PC523_002709"
     CDS             complement(10110..13229)
                     /locus_tag="PC523_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HDF2476216.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPIEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYYMPKLKNKFIDLIELAVILEPYYKDYSLEYLKNTLTNFNSKVENRALSDAIDII
                     NIVNCLLVRFNSYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINLKK
                     EYNIFDSKEEKKKERETLKILNEEENHYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLE
                     RLVEGGKYGDIEEINYSVFDNFKEISIHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIEREDKQKIARSINLIIEEIDSIL
                     NFGSCNEYDSVSNYNLRWELNLQIDEILGKLKKDGVDTEISYRAYSEKIKLGCEKIIK
                     NLVLIIITIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISQITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYNIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRQHLNKSRSLILKSVIFDTIKA
                     LNVDSKMNNNDVDKDIIKKDINENIRQRAVKGEVYHIDILKKDIKVKYFNKNYLINLD
                     IFMREENKN"
     gene            13452..14276
                     /gene="pdaA"
                     /locus_tag="PC523_002710"
     CDS             13452..14276
                     /gene="pdaA"
                     /locus_tag="PC523_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HDF2476217.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYDAYYVGNPKEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPDLIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEREGYKLKTLDYLSNKK"
     gene            complement(14372..15157)
                     /locus_tag="PC523_002711"
     CDS             complement(14372..15157)
                     /locus_tag="PC523_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2476218.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVKDGNITSIILKNKSTLIIYSFKNNFDIEEMKNHIKNLDVESISGKKCVIDRLISE
                     YKNFYEKLDNKFCVLKEMKEIDFSNMKGYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKEHLKEGNVRAYFIRKNDTMISTVSTGMETSFLAMVVSISTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDIAGKIYHNIGYKEVNEWTILFK"
BASE COUNT         5139 a         2525 c         1561 g         6340 t
ORIGIN      
        1 atttgcattt ttttctttca gtttttattt taaatttttt ttattttatt ttgttttaag
       61 tattcaagat acttatctta attttatttt ttatataaaa ttcattataa ttggaattag
      121 tattactgta attgtaccag aaactccaat agaaacacca ctcatagctc cctctatttc
      181 tccaaattca aaagcttttg tggttcctaa agcatgtgca gacgttccaa gagcaattcc
      241 tttagcaact ggatgattta tattcccaag tttgaatact gtagaagcaa tcataccgcc
      301 aaaaatacct gtaaaaatta tggctacaac tgttattgat tctataccat ttaaagaatt
      361 tgctaaagat attcccattg gtgttgttat agattttgga atcagtgaat ttattatact
      421 tacatctgaa ttagctattt tgccaattat caatacagat acaaaagaaa tcactatacc
      481 acaacctatt cccgcaatga tctcaaacat atgcttttta aatagttcaa attgtttata
      541 aagcggaact gcaagcacta ctgtgacagg acctaaaaag aaatttagac tatctgcccc
      601 tatcttataa ttctcataag gaattttaga tatacttaaa aataatatta ttcctatgat
      661 tgcaattaat agaggattac atataggatt acttgttttt ctttgaatat atcttcctaa
      721 attaaaaaat actattgtta ctattatacc aaatactggc gtgtgcaata cattatacat
      781 ttttttgtcc ttcttccttc ttttttatca tcttttccac tataattcca gtagtataca
      841 ttactattag tgtacttaaa catatgacta ataaaagtac taatacattt tcccttatta
      901 aatctaatga ctttataagt gatacacctg ctggaataaa aaatatagcc atattatcga
      961 ggaaaaatcc acttacattt tctatactac tcaaatctaa aactttaaat tgtaatagta
     1021 gaaataatag cagcatacca actatactcc caggaataag tataattcct tgtataaagg
     1081 aacttatata ctctcctatt acccagatag ataaaattat tactaattga ttgaaaactt
     1141 tcattttcca cctccaaaaa ctaaagagat tgcttcaaat catatatttg tagttttatc
     1201 aacaagcaaa tatctttctt taaaatagaa agttataatt tgatacaatc tctttttaaa
     1261 tttattttta ttttaattta ataaaataca tacttggttg tttcaaaaaa cttaactata
     1321 taaacctatt tttcacttaa aatttctttt atttttgaat tgttatacat actgtctgga
     1381 acttcacttc tcaaactata aaaagtctgt tgtgcatttt ttaaatcgcc attatcataa
     1441 taaagcatac ccaactcgta atatgaatca tcataatact ggtcattttt agtataagta
     1501 tttatatatt ttttgtaata ttttatagct tcatccttat ttcccaataa cttatttgtt
     1561 attgctaatt gatatattga ttcagaaata tattttttag tttttccaga agaaactata
     1621 cttttgaaat tttctatagc tttttcatat tctttctttt tctttaaatc tagagcttga
     1681 taatataaat cttcctcatc agctacagtt atctttcttt ctggttcttt atttttaacc
     1741 tcatttaatt ccttattcgt tttttctaat tttttactcg tttcccctaa ctttttatca
     1801 gtttcactta acttttcttc tttgctactt atttgattat ttaaattttc tattcttttt
     1861 ttgctttgaa ttaaatataa acccatgcct attaaaacac aaccaactat aatatatggt
     1921 aaaatatttt tctttctttt tacttttttt gtttcttcat ttttacttat gtcttctttt
     1981 tcttcttcat ttttttcaaa aacaggatta tctttatcta attctttaag tttgtctaaa
     2041 tatccctctc tcttttttac attacctagt ttatcatata gcaaagataa aattacatat
     2101 ggttctatta gttctggttc ttcttcaatt atgttctcta gaatatgtat tgactcataa
     2161 tttaattctt catttataaa tcttatagaa tgattgtatc ttcctaaaaa agttttaaaa
     2221 tcttcagatg aaattagatt tacatacttt ctagatatat gattattttc ataacataaa
     2281 gcagtataaa aactctcaaa tgctttggca aaatcacatt ttaataactt taaaagacct
     2341 ctaagattta atatatcaat gtctttagga tttatctcta gagctttatt taccatatta
     2401 atggcactag ttatataatt tttatatgct aaatccaatg cattattata tcttttatat
     2461 gattctaatt ttaactcctt agatgttgtg tctgactttg aatttaaagc tccttgcaag
     2521 tttaataatg tttcttcttt ataattcgta gttacataac tctgttttaa tgcttttaat
     2581 gtttcatcac catcttcttc ttctatatta aaaaaagtta aatcactttt attatcttct
     2641 atcaaagata tatctaaaat ttttctaggc atgctttcta tatcttgttt tatatcttta
     2701 actaaaataa tactcacctc cactctaaaa tatatattta agataacata tttaatatgt
     2761 caagtcaaaa ctatacatat ttttccacta ttttcataat aaaaaaggta atcctcttct
     2821 tttcataaaa aggttacctc tttttcaata ctattaactt ttaatgttga atttttttac
     2881 ttttctatag agtgtagcta tacctatatt caatgcctca gctacttttt tcattccttc
     2941 tgtgttgctt ccatattttt ttatagcatt ttctatagct tccctctcaa ggtcatctat
     3001 agttttaact tcttgtttat aaagttcatc tagtttaaaa gatattaaat cagacatact
     3061 ccttacaaaa gcagttaact ccttatcttt tgaaagtatc ttttcttttt gttcttcatt
     3121 aaacgcagct atacccaaga ttccaagtat atgctctccc attttaacgg gagtacatat
     3181 atcagctaac tctttacaat tttctctatt tgcacattta ttacagttct cttctatttc
     3241 cttatttata acataggatt ttccatcttt taaaacaaga gagtacactg acttctctga
     3301 tattgtctct cctattttac ccctgaatat tcctgtacct gctatcctag ttaaatcttt
     3361 tgtaaccaaa gtaacatcta catccaagac attagaaata ttctcgcaaa tactttgaac
     3421 gtaattcgtt atattattta aattcaaagt aatccctcct atttagattt atatttttta
     3481 ttactctaca taaatcctat tcttgagaac tttaatcttg cggttgtggt ggtgtctcat
     3541 taggcttatt atcagttggt ggtgtttctg gtttttgttg attgtttgta ttttgattat
     3601 tcttattttc gtctgtactt ggtttttcct ctggttttgg ttccatagta ccaactaata
     3661 taataccttc ctttttagga taatatgaat tagctatttt ttcagtttta attttcttac
     3721 catttgcatc gttaaatgtt ctatatgttg atacagaata tgcatttctt ccacttttat
     3781 ctactttttc tttaccttta ggcattgtag ggtcattaac tcttttcatt gtagtctgtg
     3841 aaacaccatc taattttgtt gatattgtta tattttgttt atcttctgca cttccatata
     3901 tgttacaaac aatttgattt cctgaaacgt aattttttat gtaaaccgga tgctttagat
     3961 tatttttaaa caagaaatct ataccactat ctgcaacagt tgcatctctt ccttttggaa
     4021 catagcttga tggtatagta tgatttttta cattagcaat ttctagacct gcatataaca
     4081 cagaattgta taatgtagat gaaacttgac aaactcctcc accataatct tcttccatct
     4141 caccttccat tattacaggt gcactcttat acccattttc cttacttctt ttccctgtat
     4201 gctcgttaaa tgagaaagtt tctcctggca taagtaatat attacttatg ctatccgttg
     4261 ctaattttat gtttatactt ctatttattt gggaagtctt aaatgttgtt gaatacttac
     4321 ctaagtttgt atctatgtta gcaagttgct ctttttttac ttttgctgga gtagctttta
     4381 ctgctaactt atttttatag ttaccttttt tagtttgatt atcaaaagtc tccatactct
     4441 tggaaacatc cattttaaga ccatcagaat ctggtataac cttaactttt tcaccactta
     4501 tatctaatgt agcatctttt acatctacat ctatttcttt agctattttt cccatttctg
     4561 cttttaattt atttttatca taatttacca caactactaa gttgctcttc ttgccaaaat
     4621 ttgctgaaat agtttttgat agattaccaa agaaaccact ctttctattt aaattatatg
     4681 cgttttctac tgttttatcc aggtcagtag ataaatttaa atccttgctt tttactttcc
     4741 agcttttatc attgtaatta aactcaacat tctctaattt gtatttttta gctaattcct
     4801 gttttgcttg cgatttagtt aattttccaa tatttactcc attaacatat gtgttttttg
     4861 ctattttctc ccctttaaat tgcatgcaca ttatgcctat aaaaattatt gctactaatg
     4921 ccacagctcc aatagctata actagctttt tgttcatatt actcatccca tacttctcct
     4981 tacaataaaa ttatttacat atccctatta ttatattact acataatgcg ccttaaaacc
     5041 ccaaaaactt tttaattata tattaattta atattattaa ttaatattcg tttagtccat
     5101 tttctgatac agcatatatt ttttcattgt aaatattaat agcataaact tcttttcctc
     5161 taaataagcc tttatttact acagcataat aataaatatt tttatatttt ccaaacaatt
     5221 cttctaagct ctttttactt tttgaatatc tatcagcttt ctctatatct aatatctcaa
     5281 tcttcttata cttattttct ttttcatcaa gagcattagt taacacaaat ttagctttat
     5341 cttttgtaat accaaattga taacttccat cttctaatat tctaaagtat ccatcaccat
     5401 aatctacttt gtaatctttt ttcccatctt tatacgaata taaatcttta gtttttgcac
     5461 ttaccaaaac ttcatttatt aatttatcgt actcatatac ttcagcataa tagtatttat
     5521 tgttttttat catctgtgga agtgagttta tagataaatt tgccttgtct ttaccgcttt
     5581 ttttagctat tacatttata atgtcatcag atgttaaagt atacaatgat aaattctttt
     5641 tatatttttt ttctaaactc tgcacctttt catcatctga ataatcttct tttaaatatt
     5701 caatatattg taatgctctt tcataatctc cagaagtatt tgcctcttca gctctgctta
     5761 tatagtaatc atacatctct tcaatacatt gttctttccc attttgagcc atactgtaat
     5821 acttctcatc ttcttttaat actttattat acttttcaat agcttcataa tacttatata
     5881 ctttttggtc ttttactgct gagtcataaa catctctaga ctctttaatt gtttctatat
     5941 tctgtttgta tacatctagg ttactaccca taccatttaa tagcgaaaca gtgtttatgt
     6001 agttaatggc aatatcataa gtggtatttt cttctttata catttctcta actctatcag
     6061 cttgagctaa taagtcgtcc acatctatac ttattttttt taattctttc actgtattta
     6121 ttagtccaat aaagctttct tgacttatat ctccatctaa atatttatca ccactattta
     6181 atagtaattt atttattttt ttagaaacag acttattaaa tctttccatt tttgaatcag
     6241 aaaacttttt ttctatactt gtgtagtaat caacagctat agaatactgt cttttttcta
     6301 taatctttaa cagctcatta gcttcttttc ctacactaaa ctcgttacta cttaagaaaa
     6361 atactatcat caaaacaatt acacctaatg ttgataatga aattgcaact atagatttcc
     6421 ttttttcatc aataaaatct acagccttct caaataaatc tctaaaattc attatagttc
     6481 cctctccatt taataatctt tatattttat ggtaaactaa atttcttatt ggttattata
     6541 tcataattct tgaaaaaaaa taaaaacaca agttaaatcg tgtttttatt ttacctatca
     6601 atcattattt tatacataaa tatttccttt acataaatta tgatatcttt ctttttatat
     6661 ctctagaatg actcttttgt tttttattag tactgacatt tttcattctt gttttatgta
     6721 tagttttgct atcaaatatt tttctagcat tgcataaaac aattccaaat gttaaacaaa
     6781 ctgtaagcac acttcccatc aaaaagtaat aatccaatcc tatatggact cccataactg
     6841 aatttattaa tactaatgca aaagtcattg caaaacatat catcatggag ataactaaaa
     6901 ttcttagaat ttttttcaat ccactcacct cgtataatca ctaaaattga cactactcct
     6961 tatccattac tataccaaat tagtaaaaaa atagcaatta ctattgtcat ttatattagg
     7021 aaaatattaa atctttttaa taaatatctt tttttgacat aaggattact taacttttaa
     7081 taaagtagta acactaatat gtattaatta atagaataaa ataaatgata atttattgag
     7141 gggtgattta catgccaaat ttgccaagtt tagggtcaaa ggctcctgat tttaaagcca
     7201 atacaacaaa cggatctatt agactctctg attataaggg taattggatt gttttatttt
     7261 cacatcctgg tgattttaca ccagtttgta ccacagaatt tttatgtttt gctaaatatt
     7321 atgacgaatt taaaaaaaga aatacagaac taataggtct aagcgttgat agtaacagct
     7381 cacatttagc ttggatgtat aatatttctt tacttacagg tgtagaaatt ccatttccta
     7441 ttatagaaga tagagatatg agaattgcca agttatatgg catgatatca aaaccaatga
     7501 gtgatacatc aactgttcgc tctgtattta ttatagataa taatcaaatt ctaagaacaa
     7561 ttctttatta tccactaact acaggaagaa atattccaga aatacttaga atagtagatg
     7621 cacttcagac tagtgataga gataatatag ttactcctgc aaactggttt cctggaatgc
     7681 cagtgatttt accttatcct aaaaactata aggaattgaa aaatagagtt aacagttgta
     7741 ataagaaata ttcatgtatg gactggtact tatgttttgt accagataat tataatgctg
     7801 aagaagtgaa caagaaaatt gataatactt gtagctggaa aaaagaacat actaaaaaca
     7861 ttgaaaatga atgtaattgt gaacatgaac atcatgatta catgaacaaa gctcttgact
     7921 gtaaacaaga acacatgact gatattaaag atgattgcaa ccaggagaaa aaacatacta
     7981 aaaatactaa caaaactcac agttccaaac aaaataagtt taaagataaa tcttgtgatg
     8041 aaatgaagtt taactatgac aaagatgaat cttgcgataa aataaattct agctatgaca
     8101 aagatgatag tagttatgaa tatttctata aacacaatta taaaaactac gattatacta
     8161 gtgaaaaaaa tactaaaaaa atagctatga aaactttaaa agattcaaaa aaactagtta
     8221 gaccacaaat aacagaccca tacaacccaa tagttgaaaa tgcaaactgt ccagatataa
     8281 atccaattgt agcagaatat gttcttggaa atccaactaa tgtagatgct caactgttgg
     8341 atgcggttat atttgccttt gctgaaatag accaatctgg aaatttattt attccttatc
     8401 ctaggttttt aaaccaacta cttgctctta aagctgaaaa acctagctta aaagtaattg
     8461 tagctattgg aggttgggga gctgaaggat tctctgatgc agcattaaca cctacatcta
     8521 gatataattt tgcaagacag gttaatcaga tgataaatga atatgcttta gatggaatag
     8581 atatagactg ggaatatcct ggaagtagtg catctggaat agtatcaaga cctcaagata
     8641 gagaaaactt tacactttta ttaacagcta taagagatgt tataggagat gaaaaatggc
     8701 ttagtgtagc tggaacagga gatagaggat atataaattc aagtgctgaa atagataaaa
     8761 tagctccaat aatagattat tttaatctta tgagttatga ttttacagca ggtgaaacag
     8821 gtccaaatgc tagaaaacat caatcaaacc tttttgattc tgatttatcc ttaccaggat
     8881 atagtgttga tgcaatggta agaaatcttg aaaatgctgg aatgccttct gaaaaaattc
     8941 ttcttggcat accattttat ggaagattag gtgctactat aacaagaact tatgatgaac
     9001 ttagaagaga ttatataaat aaaaatggat atgagtatag atttgacaat actgctcaag
     9061 tcccattttt agttaaggat ggagagtttg caatggccta tgatgattct ttatccatat
     9121 tcttaaaaac tcaatacgtt cttagaaatt gtttaggtgg tgtattctca tggacatcaa
     9181 cttatgacca agcaaatata ttggctagaa ccatgtctat tggtataaat gaccctgagg
     9241 tattaaaaga agaacttgaa ggtatttatg ggcaattcta atagaatgta gtaagatttt
     9301 atagttatat aaaaaagcag attttattat attaaataag ctgtgtctaa ttaacactga
     9361 tgttaattgt ttataaatta gatacttatt ttctcataat ataatctgct ttttaaatca
     9421 aattttattt aattataaat taggggttta gatatactct tgtagcatgt cattatgtat
     9481 aaatattttt ttattataca ccaatagctt ttttagctaa tttatcagct tcttcattat
     9541 acttatttcc agaatgagct ttaacctttg taaattttac attaagctta ttcttaatgc
     9601 tatcataaaa atttttatat gctatagttc cttctttatt tgttttccaa actccagtac
     9661 accacttttc aataccttca tagtcaaaat ataaaatcaa gttttgaaca ttattgtcaa
     9721 tacagtactg cattgcaatt ttagaagctt ctatctctcc agctacattt ctcataccta
     9781 ctagagtttc ctcttttcct ttttcactat aagtttttat aacttcatta ttttttagaa
     9841 tcacaacacc tgaaccatac atctttattg aatgttcata acttccatct acatatgctt
     9901 ctataaattc cttatcttct ttaaaactaa atttttctgc cccataaata tattctttag
     9961 cttcttgaaa agtagaaaaa ctcttatatt cagctcctga gaatccattt acttgctttt
    10021 tgcactcatc ccatgtatta tatataccta tatttttacc ttttttcaca gcataaaact
    10081 tttgttttgc caaatatatc actccactct taatttttat tttcttctct cataaatata
    10141 tctaaattta taagataatt tttattaaaa tattttactt taatatcttt tttcaaaata
    10201 tcaatatgat atacttctcc ttttacagct ctttgtctta tattttcatt aatatctttt
    10261 tttataatat ctttatcaac atcattgtta ttcattttag aatcaacatt caacgctttt
    10321 atagtatcaa atataacaga ttttagtatt aagcttctag atttatttaa atgttgtctt
    10381 atataagtat aaaactcatt gctattaacc tttgaaattt tacaatcgtg taaatctttc
    10441 tctaatctct ttaacacact tatattagtt cctacatcaa aaatgacaaa acatccatag
    10501 tcgtatttac ttcttaaaat tctccccata gcttgcttta cctttatggt catttgagga
    10561 taatttatag tatagtagtc tatattatac ttcttcatta tagtaaaata caaagggtct
    10621 tttggattta gattcggaag cttgtctaaa gttacacaaa ccaaaccatc ccctggaata
    10681 tctactcctt caaaacatcc ttttgagcca agtactacac actttctatt taaatcgtta
    10741 aggtgcctaa ttcctttttt atcagcataa atttctaaat taaaactatg taagtatttt
    10801 ttcaatattt cataagtctt ttcttgtcta tctttactat taaaaagagc taatgtatga
    10861 ccttctgtaa tctgtgatat attagatata attttactca tttcatttgg aaattcagaa
    10921 tttctgtact cacaaatatc agaaaaccca actacagaga ctctattttt ataatcaaaa
    10981 attggttcta taatcttttc cacattctta actctgtcta ttcctaatgt atttttaaaa
    11041 taagacatac tttcagataa actcaaagtt gcagataaaa atattccttt ctctaattga
    11101 gataaaatat tttcttcaaa taaatctgct atttttaatg gcactactcg aaattcaaag
    11161 tcattataat tcttatcaat ttcaactatc cttgcataat catctttttc atcatactct
    11221 aaaaagattt caaatataat ctttatatcc tctaaatctc ttgtttttgc ctttccaaat
    11281 ttatatatat ctgcttcttt atctatacta tcatcatcaa tattcctata aattgttatt
    11341 attattaaaa ctaaattttt tattattttt tcgcaaccca attttatctt ttcactataa
    11401 gctctataac ttatctcagt atctacacca tcttttttta gttttcccaa tatttcatct
    11461 atttgcaaat tcagttccca tcttaaatta taattgctta cactatcata ttcattacaa
    11521 ctaccaaaat ttaatattga atctatctct tcaataataa gatttatact tctagcaatt
    11581 ttttgtttat cttccctttc aatttttaat acattataaa atttatcaaa agcctttatt
    11641 ttcctcatat ttctgctata ttttttatat ataaagctac tattttgaat aaactcgtaa
    11701 ggataaattt cttgtaataa ataccttaag gactttgagt ttataatgct tgaaaagaaa
    11761 tcatatcctt tttcagtcaa attatgtgct tcgtctacta ttatattttc taaaggtttt
    11821 tcatccttat acggccattt agctaatagt gaatgattta ctactgttat atgttcttct
    11881 ttcaattctt ctattctgtt tttatataga caatcttttt tgcatttttt aggtctacac
    11941 atatttgggt cacaacttac atttcttaag tgaatactta tttccttgaa gttgtcaaac
    12001 accgaataat ttatttcttc tatatctcca tacttaccac cttcaactaa tctctccaaa
    12061 aatattaaag ataaaatttc tcctttagta ggattttgag actcataatc atctatatat
    12121 gcttccagtc tatctataca aatatagtta ttttttccct ttatatatcc ataacttact
    12181 tttccattta gccctaaaga atctaacacg tttggaatat ctttatttat taattgtatc
    12241 tgcaattcct ttgtatcagt agaaattaat aatctttttt tattaattct agcttctaat
    12301 attgcaggca gtaaatatcc tacactttta cctataccag ttggcgcttc tatacatgca
    12361 attttttctt catcttcgct atttctaaac aactctctta ttgttttagt aagctcatat
    12421 tgaccaggcc tatattcata tgtaaatcct tctttattct cccatatagt tttgtatttt
    12481 aataattctt cataatgatt ttcttcttca tttaaaattt ttaaggtttc tctttccttc
    12541 tttttttctt ctttcgagtc aaatatatta tattcttttt tgaggtttat attatttgat
    12601 aaatcataat ttgcctcttc taaaaatttg ctccattccc atgttgggag attaaatttt
    12661 ttaagatatg agtttatttt aaaactcaat ggttccaaag tagttttttc atagctatta
    12721 aatcttacta gaaggcaatt tacaatattt attatatcaa tagcatctga caaagctcta
    12781 ttttccacct tagaattaaa atttgttaaa gtatttttta agtattctaa actataatct
    12841 ttataatatg gttctaaaat aactgctaat tcaattaaat caataaactt attttttaac
    12901 tttggcatat aatactcaaa aaactttttt ttcagactta aatcatgaca tataatgaaa
    12961 ctatcttcta caaaatttac aagtctatct tccaccagtt ctatttcatt agctatttct
    13021 aaatcatttt tatctaaatt tttacatact gagaatactt ctataggtac ctcaaatttg
    13081 ttttttatta atgattcata ggtatatatt ttccagtctt taaccttgac agctccaact
    13141 tctaaaattt ctgaatttaa acaatctaat ccactaactt ctatatctaa aaaaacaaca
    13201 tcattcaata cacttattat attatccatt tttatatccc ttcaagttaa acccgcctaa
    13261 cttatttaca atcatttctc tttatatttt atcatttata tacactttat ttaagataat
    13321 taatagtaac tttatatatc ttacagtaaa cttctttcat ttattatttg tatatatgaa
    13381 tatttttaac tataaatatt caaactatat tataagaaat atactttaaa ttttaaataa
    13441 gaggtgaaat tgtgaaaaaa gatagcctaa aaaaatacat tatgatagga gcatttgccc
    13501 taatactttt tggaattgct agtataaatt ttaaatcatt agataaaaca aaaactcaaa
    13561 tttctagtcc aacactcgac acacatgaat atgattggta ttttaaccct agagaggatg
    13621 gtaaacagcc atcacctata aaagaagctg atttttttaa aaaatatgat gcttattatg
    13681 taggaaatcc caaagaaaaa gttatatatc tatcatttga tgctggatat gaaagtggaa
    13741 acactccaaa attattagat acactaaaaa aacacaatgc taaagcacaa tttttcgtag
    13801 ttgaaagtta tataaaaagc aatccagatt taataaagcg tatggaaaaa gaaggacatt
    13861 tagtttgtaa tcattcaaaa agccatccat caatggcagg tattacagat tttgaaaaat
    13921 ttaaagaaga aattacaagc gtagaaaaag catacaaaga tgttactgga aaagaaatgc
    13981 ctaagtattt tagacctcca atgggtaaat ttagcgaaca atctttaaaa tatacacaag
    14041 atttaggata taaatcaata ttttggagtt ttgcttatgt agattggtat gaaaagaaac
    14101 aacctactca tgaatttgcc aaaaacaaaa tttattcaag aactcatcct ggtgcaatag
    14161 ttttactgca tccaaattca tcaacaaata ctgagatatt agatgaggtt cttacacatt
    14221 gggaaagaga aggttataaa cttaaaacac ttgattattt aagtaataaa aaataatatc
    14281 ataaattaaa aaaaactcca tcatgaatta tagtaaaata tagctattta taaatacttt
    14341 aattctcgat agagtttttt attttttata gttatttaaa aagaattgtc cattcattta
    14401 cctctttata tcctatatta tgatatattt tacctgctat atcattgtta taaaaaagac
    14461 atggaacttt tccttccagc aataattctt tactcaaatt gtaaaccatg taacttgcaa
    14521 gtccttttcc cctatatctt ttatctgtac ttatagagac caccattgcc aaaaaactag
    14581 tttccatacc agttgacaca gtactaatca tagtatcatt ctttctaata aaatatgctc
    14641 ttacattccc ctcttttaaa tgctcttttc tttcttctat ataattttta cttactttat
    14701 aatctgacct atttaaaagt tttcctattt catcaatatc tttttcctga gcattttcta
    14761 ttttataccc cttcatattg gaaaaatcta tttctttcat ttcttttaat acacaaaatt
    14821 tattatctag tttttcataa aaatttttgt attcacttat tagtctatct attacacact
    14881 tttttccact tatactttct acatctaaat tttttatatg attcttcatt tcttctatat
    14941 caaaattatt tttaaaacta taaattataa gtgtcgactt attttttaat attatagaag
    15001 ttatatttcc atcttttacc tgagaccata tatcttgata ttgagagcta aatccgtaat
    15061 tctcaacgta tccaattatg aataaattta aactttcttc atgtgataca tattgccaaa
    15121 attctttttc taaagtatta tctattttta caatcaagca gatatcctcc ttctatgtta
    15181 ataatgatat accttataat ttatagtatc atttaaaatc attatattct accaaatatt
    15241 ctaaattttt aaatatttat gcaatatgtt cgtatttatt tttatactct gttactgctt
    15301 cattccaagc ttcttctact gatatacctc tcttatttgc aattttttgt gctaattcta
    15361 ttattttcat agcttttctt gatatattca tatatattcc tcctctaaat tatcataagt
    15421 tattttaaag ttaaatttct atagtcatgt tatatattta tttacttata tcttactaat
    15481 tatttatgta ctaaatatga actacaatag aagaaattta gaacttaata aaacataaaa
    15541 tttatataca attcatatat atttg
//
LOCUS       DAMJVM010000084        15432 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.088, whole genome shotgun sequence.
ACCESSION   DAMJVM010000084 DAMJVM010000000
VERSION     DAMJVM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15432
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.088"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            205..762
                     /gene="efp"
                     /locus_tag="PC523_002712"
     CDS             205..762
                     /gene="efp"
                     /locus_tag="PC523_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDF2476219.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYDQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            854..1309
                     /locus_tag="PC523_002713"
     CDS             854..1309
                     /locus_tag="PC523_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2476220.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFAEAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            1471..2181
                     /locus_tag="PC523_002714"
     CDS             1471..2181
                     /locus_tag="PC523_002714"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDF2476221.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGV
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINEDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            2235..3305
                     /locus_tag="PC523_002715"
     CDS             2235..3305
                     /locus_tag="PC523_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2476222.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPTDTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYSPFTLEESVN
                     IVKKVLALFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKLGVILKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            3333..6887
                     /gene="smc"
                     /locus_tag="PC523_002716"
     CDS             3333..6887
                     /gene="smc"
                     /locus_tag="PC523_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDF2476223.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDIYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKILEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEINDIKEKLNE
                     NKQYIKELENNKLSSSGELSALQENIKVLEASKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNVRDENINLEITELNKNIDIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSTNLQDSISEHSKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KINNIKHEISQLELKRESLDKDIKNIKSEIDSHESKIKDLEKSIIIKSTSIENIESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKRDNVRKDIEELDNLYNQNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEAYNSIVRDIKRISGENYELEEKNKQLEESLNYEE
                     QEIIKLQDSIATEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRNLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAKEITDII"
     gene            6914..8185
                     /gene="ftsY"
                     /locus_tag="PC523_002717"
     CDS             6914..8185
                     /gene="ftsY"
                     /locus_tag="PC523_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDF2476224.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDEEEVVDSVDSLENLEETIFSGLEE
                     EVIDKDEDIEEKTEESVNNEGTEEIENFEKIDVEMDSDGKEVENISDNDSQEEIIEEL
                     ENYDEIESKEIEAKEEKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELLEDL
                     EEILITADVGVNTTMDIIERLRDKIKQKGIIEPIKVREELKSIVEDILTNENSTLDIE
                     PAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWANRTN
                     VDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKIVDR
                     EFPEAKREVLLVVDATTGQNAVIQAKTFKEVADITGIVLTKLDGTAKGGVVLAVKSEV
                     DVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            8288..8656
                     /locus_tag="PC523_002718"
     CDS             8288..8656
                     /locus_tag="PC523_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDF2476225.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            8653..10005
                     /gene="ffh"
                     /locus_tag="PC523_002719"
     CDS             8653..10005
                     /gene="ffh"
                     /locus_tag="PC523_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDF2476226.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEADVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIARGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            10043..10315
                     /gene="rpsP"
                     /locus_tag="PC523_002720"
     CDS             10043..10315
                     /gene="rpsP"
                     /locus_tag="PC523_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDF2476227.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            10380..10607
                     /locus_tag="PC523_002721"
     CDS             10380..10607
                     /locus_tag="PC523_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDF2476228.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            10715..11230
                     /gene="rimM"
                     /locus_tag="PC523_002722"
     CDS             10715..11230
                     /gene="rimM"
                     /locus_tag="PC523_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HDF2476229.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLSVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGIEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            11239..11934
                     /gene="trmD"
                     /locus_tag="PC523_002723"
     CDS             11239..11934
                     /gene="trmD"
                     /locus_tag="PC523_002723"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDF2476230.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSIAKH
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            12108..12458
                     /gene="rplS"
                     /locus_tag="PC523_002724"
     CDS             12108..12458
                     /gene="rplS"
                     /locus_tag="PC523_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDF2476231.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            12780..13676
                     /gene="ylqF"
                     /locus_tag="PC523_002725"
     CDS             12780..13676
                     /gene="ylqF"
                     /locus_tag="PC523_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDF2476232.1"
                     /translation="MSNEYEEYLMDDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADKDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALKLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEIPENVKR"
     gene            14065..15357
                     /gene="brnQ"
                     /locus_tag="PC523_002726"
     CDS             14065..15357
                     /gene="brnQ"
                     /locus_tag="PC523_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2476233.1"
                     /translation="MGKTKDIIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIKPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKVI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVV
                     NSLPLHSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         6532 a         1491 c         2924 g         4485 t
ORIGIN      
        1 gaatatagag gataataata aaaatataga ataagtaatt tgccaaatac ttgtttatat
       61 tgtataactt atgatattat gatattagta aaaatttgta atcctgactt gacagaattc
      121 aagtaagaga tataatttta aaggcatagt aatgtaaaat ttactaaaaa ataagaaatt
      181 tattttaata tggaggaaca aaaaatggta tcagcaggtg attttagaaa aggtgttaca
      241 tttgaaaaag atggacaacc atgtttagta gttgattttc aacacgttaa gccaggtaaa
      301 ggagctgctt tcgttagaac taaatacaaa aacttaaaaa caggagctat aagagaagaa
      361 agtttcaatc caagtgaaaa attccctaag gctgttatag atacaagaca aatgcaatat
      421 ctatacaatg atggtgagtt atattatttt atggatcaag aaaattttga acaaatacca
      481 ttaaattatg atcaagttga agatgcaatt aagttcttaa aagaaaatga agttgctaca
      541 ataagatttt accaaggaca accattccaa gtggaagctc caaactttgc agagctagaa
      601 gttacagata cagagccagg tataaaaggt gatacagcaa gtaatgtaac taaagcagct
      661 acagttgaaa ctggggcagt cgttcaagtt ccattattta taaatactgg agataaagta
      721 aagatagata ctagaacagg tgaatattta tcaagagtat aaaaatttat gtatataaaa
      781 gcttatatgt cttaataata taagatatat gagcttttag tttatatata aaaaagaaag
      841 cgaggggtgt tacatgaaac atttatatga agaagttgca tacttaaaag gcctagcaga
      901 aggactagaa ataagtgctg aaagtaaaga agggaaaatg atacataaaa tagttgatgc
      961 attagaagta tttgcagagg ctattgtaac attggatgaa gaacaagaag aacttcaaga
     1021 ttttgttgaa tctatagatg aagatttagc agatttagaa gaagatattg ctgacatgga
     1081 agaagatctt tatgaagaag atgacgatga ggatgatgag gacttcagct atatagaaat
     1141 ggaatgccca aattgtggag agttagtaga aatagacgaa gatttgttat atgatgatga
     1201 agtagatgtt gtttgtcctg attgtaaggc tgtgatatta tcatcagaag atgattgtga
     1261 tgatgattgt acatgtggtg gttgttcaag ttgtgatgat agagagtaaa aattcataag
     1321 aattaaaact gcctatgatg cctatgagat attttgcttc ataggtagtt ttatttttat
     1381 gaggaaggta ttatgtattt tggttttgca atacttggac aaaaatagta cactagaatt
     1441 tatagaatag gattttggag ggatgtaaat atgaaaataa gtaaaaaatt gctagataat
     1501 atacaaagat ttgaaaatgt tataaattat aaatttaaaa ataaagaata tatactagaa
     1561 gctcttactc atagttcata ttctaatgaa aataaaaaat ataattttaa cgaaaggttg
     1621 gaatttttag gagattcggt tttaggcata gtgataagtg attatttgtt taacgaagaa
     1681 gctaatctgc ccgaagggga actaactaaa cttagagcaa atatagtttg tgaagattcg
     1741 ttaagtgaag tagctaatga tataaattta ggtgtgcaca tgttattagg tagaggagaa
     1801 gaagcaacag gaggaagaca taggacttca atattagcag atgcatttga agctgtaata
     1861 gctgctatat atcttgatgg aggatttgaa agtgcaagac agtttatact tcatcatatg
     1921 gaaaatataa tttatgattc aagaaaagga aatatattta gagactacaa aacgcatctt
     1981 caagaagtgt tacaagggaa tggagaaaat aatatctggt acaggcttat agaagaaaaa
     2041 ggaccagacc ataataaaag atttgtaatg gaagttggaa ttaatgaaga tgtattgggt
     2101 ataggagaag gcaaaagtaa aaaagaagct gaacagttgg ctgctaaaat agctttaaag
     2161 aaaaaattat gggaaaaata aatataatat gtaaaaatat aaaaagaaat aattatatta
     2221 gaaggatgtt gtagatgaaa aagagaatta tacctatatt tgttcctcac aagggatgtc
     2281 ctcatgattg catattttgt aatcaaaaaa aaataacagg tgtgagtact gatgttacta
     2341 gtgaagatgc aagaaatata attgaagaat gtttagagac tatagataaa gatgcagatg
     2401 tagaaatagc attttttggt ggtagcttta ctgctataga tattgatata caaaaaagtt
     2461 tattatcagt tgctaaagaa tatgtagaaa aaggtcttgt aaaagatatt agaatgtcta
     2521 caagaccaga ttgtattgat gaagatatat taagtatgct aaaagaatat aagacaagta
     2581 taattgaact aggagtacaa tctttagatg aaaaagtatt attagatagt ataagaggtc
     2641 atcaatctga aattgtatac aagagttcaa agatgataaa aaatagtgga attaaactcg
     2701 gattacagat gatgattggt cttccaactg atacagaaga aaagtgtata tttacagcaa
     2761 aaaaatttgt agagctaaaa ccagattgtg ttagagttta tccaactttg gtagtaaaag
     2821 acacaggact tgaaaagtta atggaacaaa ataaatatag tccatttact cttgaagaga
     2881 gtgtaaacat agtaaaaaaa gttcttgcac tgttttatgt taataatatt aatgtaataa
     2941 gagttgggct tcaagctact gatgatatac aaattggaaa agctgtttta gcaggaccat
     3001 atcatcctgc atttagagag ttagtagaag ctgatatgat aaaagattat ttagaatttg
     3061 ttattttgca aaataaaaat ataaagcaga tgttggtcaa atctaataaa aagaatattt
     3121 ctaaaatcat aggaaataaa aaaacaaatg ttaaatatat ggaagaaaaa cttggtgtga
     3181 ttttaaaaac acaagaatca gatttagata taaatcaatt agaaattgtg ttagatggaa
     3241 aaagtttaat tagtgctaat atgagagaca ttcatagaaa attgtatgac atttatgacc
     3301 tttagtttta ataatgaagg gagagaaaag atttgtattt aaaaagacta gagttaaagg
     3361 gatttaaatc ctttccagta aaaacagata ttatttttaa agaaggaata acagccatag
     3421 taggaccaaa tgggagtgga aaaagtaata tatctgatgc tgtaagatgg gtattaggag
     3481 aacagagtat taagagcctt agaggtgata aactcgaaga tgtaatattt gcaggtacag
     3541 atacaaaaaa acctatgaat tattgtgaag tggctcttac tattgataat tctgaaaatc
     3601 aattggaatt agattttaca gaagtaacta taagaagaag ggcttataga aatggtgaaa
     3661 gtgaattttt tctaaataat aaaagttgca gattaaaaga tataaaagaa gtctttttag
     3721 atactggtat tggaaaagat ggttattcaa tcatagaaca aggaaaagta gatgaaatat
     3781 taagtaataa tcctttaagc agaagaaaag tttttgatga agcttgtggc atatcaaaat
     3841 atagatataa aaaacaggaa gcagaaagga atttaagcaa tacaaaggaa aatttagaaa
     3901 gaatagatga tatatatata gaaattgaaa atcaattaaa acctcttttt aatcaacaaa
     3961 ccaaggcaaa aaaatactta gaaataagtg aaaaattaaa aatacttgaa gtaaatagct
     4021 ttataagaga aattgaagga atagaaaaag aattaagtga ggttaatgaa catcgtaagg
     4081 ttattgaaaa ggaactcaat gaaaaagaag aacaaaaaaa tgttgtagag aaaaagcaag
     4141 aagatattaa taaagaggta gaggtattac aggatgtaat tgaaaaatct gtagattata
     4201 taaattcaat caaaggagtt atatcaaaaa aagagtccca aataaattta attaaggaaa
     4261 gaataagaaa ttttacgaat gaaattagta gaaaaaattt agaaataaat gatataaaag
     4321 agaaactaaa tgaaaataag caatacataa aagaattaga aaataataaa ttatcaagta
     4381 gtggagaact atctgccttg caggaaaata taaaagtatt ggaagcaagt aaagataaac
     4441 aaaaaattaa gttagaatcc ctaaataatg agatagaatt attgaaagaa agtataattg
     4501 atatattaaa taagaaacaa gagtttagta ataagttgtc cactttaaac gcaaataaag
     4561 aaaatatgaa tgttagagat gaaaatataa atttggagat aacagaacta aacaaaaata
     4621 ttgatataaa atcatctgaa ttagatacta taaataaaga gtttaatatg caaaatgaaa
     4681 atttaaagaa tgtaaataat aggcataaag aattatcaac taatttgcaa gattcaatta
     4741 gtgagcacag taaactagaa gatgaaatac agaaaagtaa atataatcta aatggatata
     4801 attcaaaatt aaatgtctat atagatatgg aaaatcatta tgaaggattt aatagaggag
     4861 taaaagaagt actaaaaaat aaaaatttaa aaggtgtaca tggagcatta ggacaaatta
     4921 taaatgtacc tgaaaaatat gagaaatcta tagaagcagc acttggtgct tatatgcaaa
     4981 atattataac tgataatgag tttagtgcta agtctgcaat aaactatcta aaacagaata
     5041 atttgggtag agtaacattt cttccattaa atataattaa atcaaataaa ataagcctag
     5101 gaaatctaaa agcaaatact aagttcatag gaatagcaag tgatttaatt acatttgatg
     5161 aaaaatatag aaatataatt gaaaatatac ttggaagaac aatacttata aataatatag
     5221 atgaggggat aaaatttgca aaagaaactg gacataggtt taaaatagta actttagatg
     5281 gtgaaatctt aaatccagga ggttctttga ctggaggaag tctaaagact aatgggaata
     5341 tactatcaag aaaaagatac attaatgaat atacagaaaa gataaataat atcaagcatg
     5401 aaatttcaca attagaatta aaaagagaat ctctagataa agatataaaa aatataaaga
     5461 gtgaaattga ttcacatgaa agtaaaataa aagatttaga aaaaagtata ataataaaaa
     5521 gtacaagcat agagaatatt gaatctgaaa ttgaatcgtt aaaaggtagt attactaaat
     5581 tagagaatga gaaaaatgat ttaaattcta acctaaatta tacacttgaa aaaagagata
     5641 atgttagaaa agatatagaa gagttagata atttatacaa tcaaaataaa gaaaaaattg
     5701 atgcattgaa tgaggaaata aaaagataca atgatttata tgataaagaa aaatctgaat
     5761 ttgatgaatt aaacttaagc ttagtgaaga aaacagaagc ttacaatagt atagttagag
     5821 atataaaaag aatatctggt gaaaattatg agctagaaga aaagaataaa caattagaag
     5881 agtcattaaa ctatgaagaa caggaaataa ttaaattaca agattctata gctactgaag
     5941 aaaaagaaaa agaaaatcta acaaaacaac taggagatag taatagaaat cttgaaacta
     6001 gaaaaatagc aaaagatgat ttgaaaaata gttttgatga aattaataaa gaattaaaaa
     6061 ctatagatag acagcatata gagcttaaag aaagtttatt taaagttggt ggaagattag
     6121 aaagattaaa aactagtcaa gacacatata taaataagtt gtttgagcaa tatgatatga
     6181 ctttagtaca agcattggaa attaaagatg aagatttgga tatagataga aaattcttag
     6241 aaagtctaaa gagagaaata agaaatcttg gaaatataaa tatagactct ataaaggaat
     6301 atgaggaaat aaaagaaaga tacgattttt atagtgaaca aaagcaagat ttagaagagt
     6361 ctatggaaga gatagaaaaa cttatacata ctttagaaga aaatatgaaa tctgaatttg
     6421 aaattaagtt tgaagaaata agtaaaaatt ttaaatatgt atataagaga ttatttggtg
     6481 gtggttgtgg agaattaact atcttagata aagaaaattt attagaaagt gatatactaa
     6541 taacagctca acccccaggt aaaaaaatga aaaatttaaa cctcttatct ggtggagaaa
     6601 aagctttaac ggcaataagt atattatttg ctatattgat tacaaagcca acaccgtttt
     6661 gtatattaga cgagattgaa gctccacttg atgatgcaaa tatatttaga tttggagagt
     6721 ttttgaagga tttatctaaa gagactcaat ttatatcagt aactcatagg agaggaacta
     6781 tggaagctgc tgattatatc tatggagtta caatgcaaga aaaagccatt tcaaaagtta
     6841 ttagtttaaa attaaaagaa gctaaggaaa taaccgatat tatataactt agattgacat
     6901 aggaggaata acagtgttaa aaaaattatt tggttttgga aaagacaagg aaaaggaaat
     6961 agaaaagaaa gatgaagaag aagtagtaga cagtgttgat agtctagaaa acttggaaga
     7021 aaccattttt tcaggacttg aagaagaagt gatagataaa gacgaagata ttgaagaaaa
     7081 aactgaggaa agtgtcaata atgaaggcac tgaagaaatc gaaaactttg aaaaaataga
     7141 cgttgaaatg gatagtgatg gaaaagaagt agaaaatatt tcagataatg attctcaaga
     7201 agagataatc gaagaattag aaaattatga tgaaattgag tctaaagaga tagaagctaa
     7261 agaagaaaag aaagttaact tgtttgaaag actaaaacaa ggattgacaa aagcaaagca
     7321 aggaataact gatagaatag atgaggtttt aaaatcatat acaaaaatag atgaagaatt
     7381 acttgaagat ttagaggaaa ttttaataac agcagatgtt ggtgtaaata caactatgga
     7441 tataattgaa agactaagag ataaaataaa gcaaaaaggt attattgaac caatcaaggt
     7501 aagagaagaa cttaaatcta tagttgaaga tattcttaca aatgaaaact ctacactaga
     7561 tatagaacca gcaccatgta taatattaat ggtgggtgta aatggggtcg gtaagactac
     7621 tacaataggt aagctagcaa atagatacaa aaaagatggt aaaaaagttt tattagcagc
     7681 agcagataca tttagagctg cagcaacaga gcaattagaa atttgggcaa atagaaccaa
     7741 tgtagacata ataaaacacc aagaaggagc agaccccgga gctgtagtat ttgatgctat
     7801 aaaagccgca aaagctagaa aaacagatgt attaatatgt gatacagcag ggagattaca
     7861 caataaggct aatcttatga atgaacttgg aaaagtattt aaaatagtgg atagagaatt
     7921 tccagaagca aagcgtgaag tacttcttgt ggtagatgct acaacaggtc aaaatgcagt
     7981 tattcaagca aaaaccttta aagaagttgc agatataaca ggtatagtac ttacaaaact
     8041 tgatggaact gcaaaaggtg gcgtagtgct tgcagtaaaa tcagaagtag atgtgcctgt
     8101 aaaacttatt ggagttggag aaagtgtgga agacctacaa gatttcaatg caaaatcatt
     8161 ttcagatgca ttatttggta actagtgttc ttttaaaaat aatggttgac aagagtgttt
     8221 ttatgatata aaatacaata gtgtaaagta aataaccttt acagtatact gctaggaagt
     8281 gtaagatatg aatatagaaa aaatggtcga aataggattg ttatttgaac agtataaaga
     8341 attattaact gacaagcaga aagaaatagt tgctctatac tatgaagaag attattcttt
     8401 aggtgaaatt agtgagaatc tgaatgtatc aagacaaggt gtgtatgata ccttaaagcg
     8461 ttcagaaaaa atactaagag attatgaaga gaagttacac ttagtatcaa agattcaaga
     8521 gcaggaaaaa aatattaaga ttataaaaga taaaattatt gacattaaag aagatttatt
     8581 gcacaataga gattgtgcta atttaatccc taagctagaa aatatagaag atgtatgtag
     8641 ggagatgata aaatgatatt tgaaggattg tctgataaac tacaaggggc atttagtaag
     8701 ttaaaatcta aaggaaaact tacagaagca gatgtaaaaa atgctatgag agaagttaaa
     8761 cttgctcttc ttgaggcaga tgttaacttt aaagtagtta aagatttcat aaaaaaagtt
     8821 caagagagat gtgttggaca agaagttatg caaagcttaa ctccagctca gcatgttatc
     8881 aaaattgtaa atgaagagct tacaagtcta atgggagatg tacaaagtaa agtaatgatt
     8941 tcctcaaagc ctcctacaat tttaatgatg gttggattgc agggtgcagg taagactact
     9001 acagcaggaa aacttggagg atattttaaa aagcaaggta aaaaacctct attagtagct
     9061 tgtgatattt atagaccagc agctataaaa caacttcaag ttgttggaga aaaattggat
     9121 atacctgtat tcaatatggg agacaaagaa aatccagtaa atattgcaaa agcaggttta
     9181 agccatgcaa ttaaaaacgc taatgattta gttattatag atacagctgg tagacttcat
     9241 atagatgaag tacttatgga tgagttaaag tctattaaat cagaagttaa gccacatgag
     9301 atacttttag ttgtagattc tatgacaggt caagatgctg taaatgttgc agaaagcttc
     9361 aatgaggcac ttggtgttga tggagtagta ttaactaaat tggatggaga tactagaggt
     9421 ggtgctgcac tttcaataag agcagttaca caaaagccta taaaatttat ggggatggga
     9481 gaaaagctag atgatataga acctttccac ccagatagaa tggcatcgag aatattaggt
     9541 atgggagatg tcttaagctt aatagaaaaa gcgcaagaaa acatagactt agagaaagct
     9601 aaggaattag agcaaaaaat taaaaaacaa gagcttgatt ttgaagattt cttacaacaa
     9661 atggaacaaa tacaaaatat gggacctctt gacaagatac ttggtatgat acctggtatg
     9721 ggaaatgtaa aagaccaact tggagatgta gatttgaatg gtaaagaaat gaagagaaca
     9781 aaagccataa ttcagtcaat gactacatat gagagacgaa acccaggagt attaaatgct
     9841 tcaagaaaga aaagaatagc tagaggcagt ggaaccagtg tacaagatgt aaatagactt
     9901 ataaaacaac ttaatgaaat gaaaaaaatg atgaagatgt ttgctggttc gcaaaagagt
     9961 atgaagaaaa ggggcggctt aggcggttta ccttttttta aataaaatat aaaaaagtaa
    10021 aaattattcg gaggtgactg taatggcagt taaaataaga ttaaaaagaa tgggagctaa
    10081 taaaaaacct ttctacagaa tagtagtagc tgattcaaga gctccaagag atggaaaatt
    10141 tatagaagaa ataggatact ataatccaat ttctgagcct aaacaagtaa gaataaacga
    10201 tgaaaaagct ataaaatggt tagctactgg tgctcagcca acagaagtag ttaaaaaatt
    10261 acttgtaaaa aacggagtaa tagaaaaatt cgaagcttct aaacaagcaa aataattttt
    10321 tcttggcaat cagtttaagt aagtactcat tgctaaggtt actttaggag atgaaatata
    10381 tgaaagagct agttgtagat atagctaagg ctctagttga taatcctgac tcagttgttg
    10441 ttgaggaatt tgaagataat gacggtatcg ttttaaagct tacagttgct caagaagata
    10501 tgggtaaggt tattgggaag cagggaagaa tagctaaagc tataagaact gttgtaagat
    10561 ctgttgcaaa tagagaaaat ataaaagttt ctcttgagat agtataaaat aggaggatta
    10621 ggtataatgc ctagtccttt ttatataaat ttgtacttta agattttatg atgatatgac
    10681 cacataagat taaatttttt aagaaggtga taacatgaaa gaaaaattaa ctcattttaa
    10741 ggtaggtcaa atagtaaata cacaaggtct aaaaggtgag gtaagagttt atccacttac
    10801 tgatgatata gagaggtttg atgaattaaa agatttctat ttgggaaaag atttaaataa
    10861 taaattgtct gttgaaaaag taagatacaa aggttctatg gttataatga agataaaagg
    10921 tatagatagc atagagaaag cagaaaaact aaaaaataaa tttatgtatg tatctagaga
    10981 agatagcaga gagcttgaag aaggcgaatt ttttatagca gatatgattg gaatagaagt
    11041 tttaaccgta gatggaaaat atgtaggaat attagaagat gttcttcagt attcagcaaa
    11101 tgatgtttat gtagtaaaag gtgaggaaga taaagaattt atgataccag ctataaaaaa
    11161 atttgtacct acaatagata ttgatgaaag aaaaatgatt atagacccta ttaagggaat
    11221 gatagattag gtggtaatat gagatttcat ataatgacac tttttccaga aatatttaat
    11281 tcgtatatgg atgaaagtat aatgaagaga gcagttgaaa aaggtataat tgaggttcat
    11341 atatataata taagggattt ttctaataat aaacataaaa aagtagatga ttatccattt
    11401 ggaggaggag ctggaatggt aatgactcct caacctatat atgacacgta taagcatata
    11461 ataacaactc ataatataaa taatccaagt gtaatttatc ttactccaaa aggtaaggta
    11521 tacaatcaat ctatagcaaa gcatatgtct ttaaaagaag atataatact gttatgtggt
    11581 cattatgaag gaatagatga gagaatcatt gatttaatag ttactgatga aatttctatt
    11641 ggagattatg tattaactgg aggagagctc ccagcactta taatgataga ttctatatca
    11701 aggttaatac caggtgtatt aaaccaagaa gaatcatttg aagaagaatc atttaaagat
    11761 aatttattgg aatatcctca ttacactaga cctagagatt ttgaaggatt aaaagtacca
    11821 gaagtgcttt tatcagggaa ccataaaaag atagatgaat ggagaagaga agaatctata
    11881 agaataacca aagaaagacg atttgattta tataaaaagt ctaatgaaaa ataaaaatat
    11941 aataaatatg tatacaataa agtacctata aatggaatta aaaacataaa agtgcttgta
    12001 ataactattg aagtatagta taatataaaa tgttgaaaat acgggcgttc ctctgtttac
    12061 actggtcggg tagttaagaa cgtctatgat atctaggagg aagaaagatg aatgaaatgc
    12121 taagagcaat tgagcaagaa caattaaaaa atgaagttcc taattttgga cctggggaca
    12181 cagttaaagt acacgttaga attatagaag gaaaaagaga aagaatacaa gtattcgaag
    12241 gtgtagtatt aaagagacaa ggtggaggag caagagaaac tttcacagtt agaaaaatgt
    12301 ctttcaatgt tggtgtagaa agaactttcc cagttcactc tccaaaaata gaaaaaatag
    12361 aagttactag aaaaggtaaa gtaagaagag ctaaactaaa ctacttaaga ggtagagtag
    12421 gtaaagctgc taagataaaa gaagctagaa ataaataatt aaaaacttta cttataaatg
    12481 gggggctgat ttatcagtcc tctttctatt ttaatgaaaa ttacagcaaa taattatact
    12541 aaaaataaat agaaatattt gttgaaaata tacattattt atctatgaat ttataacttt
    12601 taaaatgaaa aataaaattg gtaaatattg cattgtattt tgtaacaata ttaaaaataa
    12661 gagtacagta ttttgtaaag tatgacgtta aaaagatgac aatattatag tttgtgaaga
    12721 tatatttttt tagtgaaatt aggatataat aatataatat ttaataagga gtgatacata
    12781 tgtcaaatga atatgaagag tatcttatgg atgacaatct acatataaac tggtatccag
    12841 gtcatatgaa aaaaactaag gagttagtaa aaaacaactt aaaattgatt gatgtagtaa
    12901 ttgaactttt ggatgctaga ataccgttta gtagtaaaaa tccagatata gacaggcttg
    12961 taggagataa gccaagagtt gttgtactta ataagagtga catggcagat aaggataaat
    13021 tgaatcaatg gattgaatac tataaaaaaa ttaatataaa agctatacct gtagatacaa
    13081 taaaaggtgt tggtataaat aagataatcg aagaatgtaa gaatgtaact agagaaaaaa
    13141 tgagttcttt aaaagataaa ggtagaaaag aaagagctat acgaataatg atagttgggg
    13201 ttccaaatgt tggtaaatct tctcttataa acaaattaac aggtagaaaa agtacgcaaa
    13261 ctggagataa accaggagta acaaaaggga aacaatgggt tagattaaag ggaaatcttg
    13321 aacttttaga tacacctgga atactttggc ctaagtttga agaccaagaa gttgcattaa
    13381 atttagcatt tagcagagct ataaaggatg agattttaga tactgagact ttggctttaa
    13441 aacttataga aaaacttatg aaaatagaac ctgagaagtt gaaagccaga tataagctag
    13501 actgtctagg agaaactcca attgaaacta tggatatgat tggtcataaa agagggttta
    13561 taacaggtaa aaaagaacta gattatacac gtatagccac aactgtattg aatgaattta
    13621 gagacgggaa aataggaaat ataacattgg aaattccaga aaacgttaaa agataaattt
    13681 taaaacaaaa tcaaaaattt aatgttaaaa atttaaaaaa gcccttccag aaaggctttt
    13741 ttattttttt gaaatataga caagtatttc tgtataaaag tataaattag gttagaaaac
    13801 ctttaaaaat attaatttaa gacaaatata tctaatattt tgaaaattaa aatgagtgtg
    13861 attatttaaa atcttggcat attgacttgc ttgtttcgta taatatataa taagcacaaa
    13921 taaatacaat atattaaaaa tttaatttat ttaattttat taatttaata atgagttctt
    13981 attgtttaat ttaacttatt tatgcaaacg taaacaatcg agatatttta ttaatttaat
    14041 aaggttaaaa agggggattt tataatgggt aagaccaagg atatcatagt atttggattt
    14101 gcgttatttg caatgttctt tggagcaggt aacttaatat ttccacctta cttgggaata
    14161 attacaggtc cagaatggtt aattgccttc ttaggattta catttgctga tgctggattg
    14221 gcacttctag cagtaatggc tacagctaaa ttcgatggaa acgtagttga aatgtttaaa
    14281 agatgtggta tgaaattagg gattcttata ggttgtgcag atattttatg tataggacca
    14341 ttcttagcta taccaagaac tggtgcaaca acttatgaaa tgggtataat gcctttattt
    14401 ggaacttcaa ttccagtgtt attattctgt atactttttt ttgcgatttc atatgtacta
    14461 acaataaaac cttctaaggt cgtagatata gttggacagt ttttaactcc agctcttcta
    14521 atagctttag catttataat tataaaaggt attatatcac ctcttggaga tatagtagat
    14581 aaacctatga tacctaatgt atttgcagaa ggtataggtc aaggatatca gacaatggat
    14641 gcttttgcag caatagcact tgcatcagta ctaatagtat ctttaaatga taaaggatat
    14701 tctactatta gtgacaagtt aaaaatgatt ggtaaagctg gtgttcttgc ttgtggagga
    14761 ttagctctag tttatggagg attatgtttc cttggggcaa ctgtatcaac aatgtatgga
    14821 acagatgcag tacaatcaca agtaatagta aatataactg aagggttact tggaaatgtt
    14881 gggaaagtga ttttagcagt agtagtttca ttagcatgtt tgacaacatc tataggtctt
    14941 acatctgcta caggtcaata tttctctaga ctcacaaaag gaaagttgag ttatgaaaaa
    15001 atagtattag ctgtatctgt attcagtgca gtagtagcat cttttggtgt tggaactata
    15061 ataaagatag catctcctat actaagtata gtttatccac catcaattgt tttaataatt
    15121 ttagctttct tcaatgaaaa aattaagaat gataatgtgt acaaaggagc tgtatatatg
    15181 tcattgcttg taagtatact aacagttata tctagttatg gagtagctgt tccagtagta
    15241 aattctttac ctcttcattc attaggattt aactgggtgg tgccagttat aatagctgga
    15301 ataattggta attttatacc atcaaagagt caatctaaca cacttggaac aaactaagtt
    15361 ttgtttaatt aaaatctaaa agtaaaagga gttatttgta taaataactc cttttacttt
    15421 tagattttat aa
//
LOCUS       DAMJVM010000085        15125 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.089, whole genome shotgun sequence.
ACCESSION   DAMJVM010000085 DAMJVM010000000
VERSION     DAMJVM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15125
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.089"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            238..1602
                     /locus_tag="PC523_002727"
     CDS             238..1602
                     /locus_tag="PC523_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDF2476234.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSIFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLSAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKDATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
     gene            1626..2279
                     /locus_tag="PC523_002728"
     CDS             1626..2279
                     /locus_tag="PC523_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2476235.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(2398..3180)
                     /locus_tag="PC523_002729"
     CDS             complement(2398..3180)
                     /locus_tag="PC523_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDF2476236.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQTSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMVYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYAESLHVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(3297..4463)
                     /locus_tag="PC523_002730"
     CDS             complement(3297..4463)
                     /locus_tag="PC523_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2476237.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAMF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGILLTYATAMLIL
                     YLFMYFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIVGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVLFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVEGKLDKKGLPTDTSDYM"
     gene            complement(4602..5966)
                     /locus_tag="PC523_002731"
     CDS             complement(4602..5966)
                     /locus_tag="PC523_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDF2476238.1"
                     /translation="MDIKELVKIQRKYCNTGKTRDISFRIEQLKKLKSVVSQNEEKIL
                     LALKRDLDKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSIVKDIIENAFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIVMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDKKNELIEKIKYYTNKYFGDNPS
                     ANEQFPKIINQRHFSRILSLIDKDKVIYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKILEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKIL
                     KKIM"
     gene            complement(6282..6806)
                     /locus_tag="PC523_002732"
     CDS             complement(6282..6806)
                     /locus_tag="PC523_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2476239.1"
                     /translation="MKSIVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFFKKNYKELQKKNLVVFGVGAAPYDDEVIKRLREVNFKDE
                     LNKVPCFYCRGAWDETKMSFLDYTLCSFFKKSIAKKERQGLKPVEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
     gene            complement(6855..7352)
                     /locus_tag="PC523_002733"
     CDS             complement(6855..7352)
                     /locus_tag="PC523_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2476240.1"
                     /translation="MNVETLMKKFMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKGRVEEYSKKVRGIQEKVLQLLFKDFTEKEVEQLFFGIMKLSAGIENVKNYVEN
                     NDIEE"
     gene            complement(7585..8862)
                     /gene="hflX"
                     /locus_tag="PC523_002734"
     CDS             complement(7585..8862)
                     /gene="hflX"
                     /locus_tag="PC523_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDF2476241.1"
                     /translation="MDMAKKGITAGLNINNKSEDFNELMIELGNLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEELLNLIKKESIEVVVFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLVGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITFLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIILANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKNHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DEILDSIDKEGLFVSVKKDINIDKMIKCICDSIFKDYVRCKLLIPYDKGHVVSYFNEN
                     ASVISTEYREDGVILDVECSNIEYNKYKKYVLE"
     gene            complement(9591..11423)
                     /locus_tag="PC523_002735"
     CDS             complement(9591..11423)
                     /locus_tag="PC523_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2476242.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILMFLLCLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISEKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAILILPISMFVISRIVKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEDQIVENAVSIDGLKGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYNMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(11416..13662)
                     /locus_tag="PC523_002736"
     CDS             complement(11416..13662)
                     /locus_tag="PC523_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2476243.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELDKITLFMDKSSKDKVLDNYTLLNEKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNIIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFEGMPESMITQSSVSYVKSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMVSMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEEQEFKVDKKGLVEFRNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKEATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(13730..14191)
                     /locus_tag="PC523_002737"
     CDS             complement(13730..14191)
                     /locus_tag="PC523_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2476244.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNRVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEEEKYF"
     gene            complement(14496..15062)
                     /locus_tag="PC523_002738"
     CDS             complement(14496..15062)
                     /locus_tag="PC523_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HDF2476245.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT         5050 a         2567 c         1643 g         5865 t
ORIGIN      
        1 ctattgattt aaaaataaaa gcatgttaca attacaaaaa aagaatctac gcgataaata
       61 tcaatatgca aaaatttcag aattttcaat tttctatttt tatgtaattt ctatctaata
      121 tcataaatta ttatagctat attaatctaa agccaataaa acaaatatcg tactactttt
      181 atataaaata tatttaaaat atattttatt aaaatatttt ataaaggaga agagactatg
      241 ttacttttaa ttgttacgtg tgcaatatac atatggattg gtttttattt ttcaaaaaaa
      301 gttaaaacag catccgattt cctaattgca ggtagaaatc ttcctttacc tatactagct
      361 gcaaccattg ctgctacatc ttttggagga gggtgcttag ttggtggagt tcaatgggga
      421 gctgaaagag ggctttgggt tggaatgtat tctactatag gtgcagcttt agcctgcttt
      481 ataaacgcat tttttgcagg aaaattaaga agcttatctt gcgatataac acctgctgat
      541 tatattgaaa ctagatatgg tcatagtata tttttaagag cttatcaaag tcttgtaaca
      601 ccaatatcta tactagccat tatgggtagt caattaatct cttttggttc tgtgtctact
      661 gcatttggca ttccttatga tatagctgta attataggag ttatagtcat cattatatat
      721 acttatacat caggaatgtg gggagttgca gtatcagcat ttattcaact tgctatttgt
      781 atagtgtttt tgccaatagt agcttatata agcttaaaat tagttggcga caacccttta
      841 cttatgttac aatcaatggt aaaagaacca ttctttccag gaaataaatc gattagtgat
      901 tttatgtata cagttttacc attagtttta ggctcaatat tttcttatga aagtttttta
      961 agatatcaat gtgctgataa tgctaaaaat gcagttaaaa gtagtgtaat tgcaggattt
     1021 atactattat ttcttgcttt ccctatagga ataattggag ctattggtgg tagtttattt
     1081 ccaaacatat catctgttca ggttttacct caaatgatat cgactacttt gccaccttta
     1141 atctctgtat tgttcttatc tgctatattg gctgccataa tgtctactgc cgattcatta
     1201 atgacctcaa tgagtgctat catatcaaga gatatttata acaaattgtt acaccctaat
     1261 atagaattta atgatttaaa aaatactcta aagtattctc aaattgcatc agctctaaca
     1321 gctataatag gtgcaatcat agcacttaaa tttgatagtt tattagaatt attgttttgg
     1381 ccagctccac tatgtacagg tgtaatattt gcaccatttg taattggtct ttgctggaaa
     1441 gatgctacaa aaaaaggagc tatatatgct gttatagtaa gtgttatact ggcattatta
     1501 aatatgattg gtattcttac tgtatttgat agaattttgg ttcctataat aggtggttct
     1561 attacaatat ttatagttag caaattttca aaaaaagatt gaaaagagag gtatacacag
     1621 taaatatgtc aaaatcagaa aaaggcaaag aaacaaaagc tagtattata agtgctgcta
     1681 gagaattatt ttttgagcaa ggttatcata agacaacgac taggcaaatt gcagaaagag
     1741 ctaatataaa tttaggtcta ataagctatt acttttcaag taaaagcgaa ataggagcta
     1801 taatatatga agatataaga aatgaaatgc aatctttaat ctataactac catgaagaag
     1861 gtactatgat gatgttcttt cttgctatga atgctgacct ttgtttcctt ctaaaaaatg
     1921 aagcatatag aaatttttat ttggatatag cttatgaaga agccatttta agctatcaaa
     1981 gaagtgtaac cgaaatggtc atgaaaagat atgttaatga taaagaatct atagaacatg
     2041 atgagttaat actttcttgt attggtatac ttgctatgaa acctgaaata attagagttt
     2101 atacttcaca caagtatgat atatcttctc aagctgtaat aaagcatttt acaaaacagt
     2161 ttcttattaa tcttggtcat agtactgatt tatgcgaaga gattttagaa gaattgagta
     2221 aatactatat agatatagtt gacaatttta ctccaataat aactcgtgta agaacttaat
     2281 aaaattttat taaattctta gagactgaaa ttttaaaatc atgatttgat tttatgattt
     2341 cagtctttat catatttaaa gatattttat agtatctctg ttaataccat aagcatacta
     2401 cccaattata tctataaatt tctttatcca taaattccaa agacgccatt catgccctcc
     2461 tacacactct tcatattcaa catctacatg taaagactct gcatattttt taaacatctg
     2521 attattttta tataaaaaat ctctatctcc acaaaattga taaattttag gaatgtcttc
     2581 atttctctct ttaaggttct taagtatgta aagtaaatca ttctcactat ttctagcatc
     2641 ttctggtcta ccaaaatcat gaaatagaat atctgcattg gtttctattt gttgattttc
     2701 taactcatga atcatatcag gtgaaccaga aaaacttgca actgccgaaa actcttttgg
     2761 ttttgaaagt gctaacttta aagctccata tcctcccata gacatgccag ctacaaagtt
     2821 gtcctccctt ttatctgata aaggaaatat agctctaaca aatttaggta attcctcact
     2881 tacaaaagta aaatattggt gtccatatac catatctgtg taaaaactag tttctactct
     2941 tggtatcact agagctatat ttttattttt agcataatgc tctacattag tatatctaat
     3001 ccatgaggtt tggtcatctt ctaaaccatg aaataagtat aatgttttaa atttaccttc
     3061 aaaatcatat atctctttac cactaaaaag ttcttttcca tttgctacag gtaaaattac
     3121 aattatatca gtttctcctc cagtatactt agatttaaat ttacattcta taaatgccac
     3181 aaaaaacacc tcttctataa taattttata tctaaaaagc accaagagtt tgactttctc
     3241 agtgctttaa atattactta atttatatat ttttaattat acataacatt ttttagttac
     3301 atataatcgg aagtatctgt cggtaatccc tttttgtcta atttaccttc aacaatatcc
     3361 ttaattatag aataaattac tgcaaacatt ggaactccta taaccattcc aataaaacct
     3421 aataattttc ctgcaaccaa taaggagaac aaaatccaaa atgcagatat cccaattgaa
     3481 tctccaagta ttttaggccc tataatattt ccatctatct gttgaattat taatataatt
     3541 aacaataacc agaaagcttt tataggagat acaaacaata ctattagtgt agatggtatt
     3601 gctccaaata aaggtccaaa gaaaggaata atatttgtaa taccaattat aactgatatt
     3661 agtaatgtat atggcatttt aactaatgta agtattacaa atgttagaat acctacaatg
     3721 aatgaatcta atattttccc acctaagaat cttccaaatg tatagttact tctgtgagtt
     3781 aattctaaaa ttctagcagc ttgtttttct gtaaacactg cataagttat ttttttactt
     3841 aatccataga atttttcttt atcaattaat aggtatactg aaacaattaa tcctaataca
     3901 atattccata tactagaggc tacattcttt aacatacttc ctaatattgg gattaaatct
     3961 gttacaaact ttataatata tgtaacaaac tcattccatt tatcaactgc caaattaaag
     4021 tattgctcat caagatttaa atcagtcatt aatttatcaa taagcttagt tgcattgttt
     4081 acatacatag gtatatcatt tgctagtcca acaatactct caattacttg tggcaatacg
     4141 aaatacataa ataagtaaag tatcaacatg gctgttgcat atgttaataa aatacccaaa
     4201 cctctttttg aactttgttt tagttttttc aatttctcac tttttataaa aattctgtct
     4261 tcataaaact ttaaaataaa attaagaaga tatgcaatag caaatcctat tataaaaggt
     4321 tgtaatgttg taaacatagc gtctaaatta ttagaaaata catcaatttg tgatgatact
     4381 agataaaata taatactact agccgctatt ataaaggtat aaattgctat tgttgtgtat
     4441 tttgtattcc aattaatttt cattttttcc tccttatttc tatataatta aattttatca
     4501 taaaatcacc acttttataa caaatatgcc aaattaaaaa taaaatatat tcagaataca
     4561 aatattcaat tttatttata tttatttttc tacaatataa cctacattat ctttttaaga
     4621 atcttaatta atttattatc atatggagca tatctaatag gaacatccaa atttaatttt
     4681 tttaacacac tcttataatg agtaaatgta tcgaatccag cttttccatg atatgatccc
     4741 ataccacttt caccaatacc accaaaaggt aagtaattcg tagctatatg agtaactgtg
     4801 tcattgatac aacatccacc cgctggaatc atttctaaaa ttttattttc taggtactta
     4861 ttttttgtaa aaatataaag ggctaatgga tttggcattt gtataatttt ttgtattact
     4921 tcatccaaat ctttatatgt taaaattgga aatataggcc caaatatttc ttccttcatt
     4981 atattatcat cccaattaac attgtctact atagttggct ctatctttaa tgtctcctta
     5041 ttataatttc caccatagat taccttatcc ttatcaataa gagataatat acgactaaaa
     5101 tgtctctgat ttatgatttt aggaaattgc tcattagcag acggattatc tccaaaatac
     5161 ttattagtat aatattttat tttctctatt aactcatttt ttttatcttc atgcactaaa
     5221 acataatctg gagccacaca agtttgtcct gcatttatta gttttcccca agtaagtctt
     5281 ttagcagtaa ggtctatatt ggcatctttt aaaatgatac aagggctttt ccctccaagt
     5341 tctaaagtga taggagtcaa atgctctgat gctgatttca tgactatttt gccaacatta
     5401 gtgcttcctg taaaaaatat ataatcaaat ctttctaata gtaatttttc actttcttct
     5461 tgacttcctt gaacaactgc aacatattct tttgaaaaag cattttcaat tatatcttta
     5521 actatactag atgttgatac agaatattca gaaggcttta gtataacaca atttcctgct
     5581 gctaatgacc aaactaatgg tataagtgct aattggaatg gataattcca aggcgacatt
     5641 atgagagtaa caccataagg ttgtggaaca actttagata tagatggaaa attaaccata
     5701 cttgatttaa ctctttttat tttgctccat tttcttatat tctttatagc aaaatttaat
     5761 tcactataaa acatgcccac ctctgtcata aaaccttcaa aatccgattt atctaaatct
     5821 ctctttagcg ctaaaagtat tttttcttca ttttgtgata caactgactt aagtttcttc
     5881 aactgttcta tacgaaacga tatatctctt gttttacctg tattacaata ttttctttgt
     5941 attttaacta actcttttat atccattgtt aaaaatcctt tctttaagaa ttacacttat
     6001 acatttttag aaaaagattt aaatttatcc taacattatt ttttgtatca taataaattt
     6061 atctcaatat aacataaatc ttccaataaa aaaagccctc ttattaaata taagtaagct
     6121 tataaaataa tttattttag aataaacatt ttttaaaatt ataaccttat tgaaacaatt
     6181 aaattatccg tctttaataa tattattgtt ttataaataa tgtctaataa atttctatgg
     6241 aatttaactt tagataataa ataattataa atatatacct cttaaagttc tcttatataa
     6301 tcgagtattg gagttaaatt ttctttatta caccaatcaa atgcttctcc agacaataaa
     6361 ggtgcttcta ctggtttaag accttgacgt tctttttttg ctatagattt tttaaaaaaa
     6421 ctacaaagag tataatctag aaaagacatt tttgtctcat cccatgcccc acgacaataa
     6481 aagcaaggaa ctttgtttaa ttcatcctta aagtttactt ctcttaatct ctttattact
     6541 tcatcatcat atggagcagc tcctacacca aatactacta gatttttttt ctgtaattct
     6601 ttataatttt ttttaaagaa agaaattcct gctatgttac aagcataaag acctcctcct
     6661 aaaataatcg tatcatactt cataaccttt tctatattaa tattcttagt ttcaaaaata
     6721 tcacaagaca ataattctga aagccactta gcatattttt ttgttgaacc atatttactc
     6781 ttataaatca ctactatact tttcatataa attatctcct ttctgatgtc atacaaagtt
     6841 gattcgacac ttgcttattc ttcaatgtca ttattttcta cataattttt cacattttca
     6901 attcctgcac ttaatttcat aattccaaaa aataactgtt ccacttcttt ctctgtaaaa
     6961 tctttaaata aaagctgtaa tactttttct tgaatacccc ttactttttt agaatactct
     7021 tccactctac cttttgtcac aagacagata gctcgtgaat ctttttcatt ttgctcaatt
     7081 tgtataaaac ccttcttttc taaagcaata gctagttttt ttacattttg tcttgagcac
     7141 cccattaatt ctccaagttt agttaatgta agagtttcat ctacagagtc cgcaatagct
     7201 aaaagtaacc attgtttcat agttaattct gggtctaatt tatcacatgt agtttgaagt
     7261 ttattttgca ttacaaatat acttgcaaat atagcttgtt ttctactatc atcatctacc
     7321 ataaattttt tcattaaagt ttctacattc attattatac acttctcttt atcttagatt
     7381 ttaatttttt gataacttta aatagtaact agttacttta tattatagta attggttact
     7441 attatgtcaa caaatttatt tttatcataa atggtaaaac ggcatagctt gtggctatac
     7501 cgtttcataa aatcatattt tacaatcaat ttataattaa ttataaatta aatatatttt
     7561 aagctataat aattttttca aaatttactc taaaacatac tttttatact tattatattc
     7621 tatattgcta cattctacat ctaaaataac tccatcttct ctatattctg tacttataac
     7681 gctcgcattt tcgttaaagt aagaaactac atgcccttta tcatatggaa ttaaaagttt
     7741 acatcttaca taatctttaa atatactatc acaaatacat tttatcattt tatctatatt
     7801 tatatctttt ttaactgaaa caaataaacc ttctttatcg atactatcta aaatttcatc
     7861 taatacttct acatctatta aatcaatttt attatacaca tgtatcatag gtataccatc
     7921 tgcaccgatt tgttttaaag tatcttctgt cactttaata tgatttttat agcttggatt
     7981 tgaaatatca ataacatgta gcagtacatc tgcctcacat gcttcttcta atgttgacct
     8041 aaatgctttt actaaatcat gtggtaaatt acttacaaac ccaacagtat cagataataa
     8101 aaattctttg ttatttgcaa gaattatatt tctaacagaa gtttcaagtg tagcaaataa
     8161 catgtttttc tcaaatactt tcttttcttc agagttttta aatatatcta ctaatttgtt
     8221 cattattgaa gatttaccag catttgtata cccaactaaa gctacatttg gaagatttga
     8281 ttttcttctc atacttcttt gagtttctct ttggaatttt aagtcatcta actccctatt
     8341 tagaaatgtt atcttttctt ctattcttct acggtcaagt tctaattttt tctcaccaga
     8401 gcctctattt ttagtaccaa ctccaccact ttgacgccct aaattttcat ttgaacctac
     8461 aagtctaggt aacatatatt ttaaacttgc tacttctact tgaagtttag cttctctagt
     8521 ttttgccctt tgtgcaaata tatcaagtat caatgctgtt ctatctataa tttcacaatt
     8581 gagcttttct tcaagatttt ttagttgtga tgtagaaagt tcattgtaaa atacaactac
     8641 ctctatgctt tctttcttta ttaagtttaa taattcctca actttacctg tcccaatgta
     8701 aaatgctcta ttaacctgct ttgcattttg agttatactc ccaacaacat caatatcaca
     8761 tgctgaacat aagtttccaa gttcaatcat tagttcatta aagtcctcag atttattatt
     8821 tatatttagt cctgctgtta ttcctttttt cgccatatcc atattctcct tatctgctat
     8881 tttttattta atctatagca caagattatg gctctataat taaattcatc aagaatttta
     8941 ttttttatct tatccttaaa tgacacttat taaattataa attttaccat tataataacc
     9001 ataatcgtgc attattttct caatcttata taaaaaatca ctttcttaca cctcctccta
     9061 aatatattat gcagtttttt caataaaaaa actccttaca aatctaagta atacaaaaca
     9121 agctacttat atcataagaa ggattaaagt tctaaatata tattaaaaat acattagttt
     9181 aattaacttg tatgtaatta tcattttata tttaacccta ttcttaaatt taagtagtta
     9241 tttacatgat taataaaagc aataaaaata tctacttcaa tataatcatc aactattaaa
     9301 attaaatttt atttataata aaatttaaag aatataatta aatatacatt ttgataatca
     9361 cctccaacat tttccttact acatattata ataaaattaa aacgttgtaa agacataaaa
     9421 ttgtgtagat gtctcaaaat aaatagaatc ctctttttta ttgctaaaat gataaaaata
     9481 cattttgttt catacataaa agagtgcctt atgaaccaaa aagttcattt taagacactc
     9541 tcatttattt tatcttatta attaaacttc tatgctaaga tagttttttg ttacattgct
     9601 tcatcttctt caaattgaga attgtaaaga ttagcataaa atccaccttt ctttagaagt
     9661 tcctcatgat ttccttgttc aactatatct ccttcattca ttacaagaat catatcagca
     9721 tcacgtatag ttgataatct gtgggctata acaaaacttg ttctaccttc cataagatta
     9781 tccatagcct tttgaatcaa tacttctgtt ctagtatcaa cagaacttgt agcttcatct
     9841 aatattaaaa ttttaggatc agctaaaata gcacgagcta tagtcaataa ttgtttttga
     9901 ccttgagaaa cattacttgc ttcttcattt aatttcatat tatatccatc tggtaatgtc
     9961 ttaataaatc tatgaacatg tgcagattta gcagcctcta taacttcttc atctgtagca
    10021 ttcaacttac catatcttat attttccata attgaaccac taaataacca tgtatcttga
    10081 agaaccatac caaacatttc tctaagctca ctacgattaa aatctttaat attatgtcca
    10141 tcaatcagta ttgaaccgct attaacatca taaaatctca ttaaaagttt aactattgta
    10201 gttttacctg ctcctgtagg tccaacaata gcaacttttt gaccaggttt aacatttact
    10261 gaaaaatcat ttattatagt cttgtttggg ttatatccaa agttaacatt ttcaaaatca
    10321 attttacctt ttaaaccatc tattgaaact gcattttcaa caatttggtc ttcttcttcc
    10381 tcttctaaaa actcaaatac tctttcagaa gctgctgctg ttgattgcaa taaattggca
    10441 acctgtgcaa tctgagtcat tggctgtgta aagtttctta catattgtat aaatgattgt
    10501 atgtcaccaa cctcaattgt tttcttaatt gcaagccaac ctcctaagat tgataccatt
    10561 acatatccca agtttccaat aaacatcata agtggttgca ttatacctga taaaaattga
    10621 gacttccatg ctgaattata caggctatca tttaatttat caaactcttc aattacctca
    10681 tcttcacgat taaatgcttt cactattgtt tgaccactat aagtttcttc aacttgacca
    10741 tttacatttc ctaaatattc ttgttgatat ctaaagtatt tttgtgattt ctttactatt
    10801 cttgaaatta caaacattga tattggtaat attaaaattg ctaccaatgt cataattcca
    10861 cttatagata acatcattat caaaacacct atcatagttg ttactgatgt tataagctgt
    10921 gtcatacttt gatttaaact ttgatttaaa gtatctatat cattcgtaat tctcgataat
    10981 atttcaccat gcgtcttagt atcaaaatac ttcattggca atctatccaa tttttctgaa
    11041 atctcttttc ttaaattata ggatactttt tgagaaattc cactcattat aaatccttgt
    11101 ataaaagaga acagtgcact tattaaataa aggcaaagta aaaacattaa gatttttcca
    11161 attgcattaa aatctatacc tacatttcct ccagaaactt tactcactaa tccttcaaat
    11221 atctttgtag ttgccttacc taaaattttt ggacctacta tagaaaacga cgcactacca
    11281 atagcaaata caattactaa aattattgat attttaaatt tagataagta tgcaattagt
    11341 tttctcatag tccctttaaa gtcatttgct ttttgtcctg ctcccataga ttttcccata
    11401 ggacctcttc ccattctact cattagctag ctcctccttt gaaagttgtg ataaagctat
    11461 ttgctcataa acttcgcaat tttttagaag ctctttatgt gttcctattc caactatttc
    11521 tccctcattt aaaacaatta tctgttctgc atttaagata gtactaattc tttgtgcaac
    11581 tattaaaact gtactttcag cagtttttga ttttaaagcc ttacgaagag ctgcatcagt
    11641 ttttaagtca agtgctgaaa aactatcatc aaatatataa acttctggat tttttgctat
    11701 tgcccttgca atagaaagtc tttgtttttg acctccagat acatttgttc caccttgaga
    11761 aatttcagtt ttaaaagctt ctggtttaga atctataaat tctgtagctt gagctatttc
    11821 agctgctgtt ctcatttctt catctgttgc ttcttttcta ccatatttta aattggattc
    11881 tatagtacca gaaaacaata ctcccttttg aggtacatat ccaatttttt ctcttaaatc
    11941 atgctgagaa acatttttta tattaactcc accaagtaag atttctcctt cagtaacatc
    12001 aaagaaacga ggaattaaat ttataagagt tgacttacca ctaccagtac ttcctataaa
    12061 agcagttgtt tctcctggct ttgctgtaaa agatatattt gttaatatag gttcctctgc
    12121 gtctggatac atgaatgaaa catttctaaa ttcaacaaga ccctttttat ctaccttaaa
    12181 ttcttgttcc tctgattttt ctgggtcttt tatgcttata tctgacacta gtacttcatc
    12241 tatacgtgta gctgaaacgg atgctcttgg aagcatgata gataccattg atatcattaa
    12301 gaatgacatt attatttgca ttgtatattg tataaatgcc atcatatctc caacttgcat
    12361 tgctccagaa tcaacactat atgcacctct ccaaactata agaactgtga taaggttcat
    12421 tataagcatc atagccggca tcatacatgt cataactcta tttacaaaaa gatttgtttt
    12481 agttaaattt ttatttgctt tatcaaatct ttcttcttca tgtttttcag tactaaaagc
    12541 tctgataact ggaataccag taattatttc acgacttact aagtttaact tatctacaag
    12601 cttttgaact agtttaaact taggcataac cactgagaat aacactacaa ttaatgataa
    12661 aatagcaact actgctactg ctataatcca tgccattgaa gtatttgtgt ttaaaacttt
    12721 tattactcca cctatagcca ttataggtgc atagaatact attctaagca acataaccat
    12781 caacatttgt atctgttgaa tatcatttgt actacgagta attaatgaag ctgtagaaaa
    12841 ttcattcatt tcagtatttg aaaatgacat aacttttcta aagactccac ttcttaaatt
    12901 tctacctaca cttgctgcta ctcttgctgc taaaaatcct actgttattg tggctaccat
    12961 actaaccatt gctataccaa gcattttagc cccagtaaat aatatataat tattttgagt
    13021 tttttctgta tcaataccta ttttctcata ttctgacttt acatatgata ctgaactttg
    13081 tgttatcata ctctctggca taccttcaaa gcctttgctc atctctttag tcaatgtgtc
    13141 taactgttcc tttggcattg ctgatagcaa tttaaatata tcaacatctc cactttgagt
    13201 agcttgtggt ggaagtttac tcattaactg agctttcata gcttttacct ctttactatc
    13261 gccctcaagc cctgatacta tcaaaatagc cttaccaaaa ataatattta aatcatctat
    13321 agtatctttg tctttagtat ttaactcata aagagattca tcttttagac ctgggtactt
    13381 atctttatac ttaacataat ccttttcatt caacaaagta taattatcta gtactttgtc
    13441 cttactgctt ttatccataa ataaagtaat tttatctaat tcactctctc taatcacaga
    13501 tggaacagag ttttctactc caccttgttg tatacctaca tttactatat tggatgtgta
    13561 ttctggtaaa gaaaggtcac ataccgcttg aattaccaat aggcagacta ttattactat
    13621 tgataatgcc gatttcttta aatatttaat taatttaaac atacatttct tcccttctgt
    13681 aatattaatt agtctaataa cattaaattg aaaaatatta ttttaaactt taaaaatatt
    13741 tttcttcttc atgcttatga catcctgctt ttaaattatc tcgtacttgc attagaagtc
    13801 ttctaagtac tattttttct tcttcactaa atcctaaaaa aaactctctt tccaattcct
    13861 tgttaaattc ttcaactcta ttacatattt ctaaaccttt gtctgtcaaa aaaattctag
    13921 atactctttg gtccttttca tcttgttttc tttccacaag tccagctttt tccatcctct
    13981 ttatcataac tgtagttgta gatgctttta ctttcctttt atctgctaat tctttttgac
    14041 tttgcccatc tttcatcttt aaagaaaaaa gtatttgagg ttgaccatga tataagccta
    14101 atttatcaaa ataaaaatga gttcttataa aatatattcg cattaattgc aagaaaacat
    14161 catttaattt ttcttcagaa ttgctattca tacttgccct ccttattttt tatattactt
    14221 agtcggctaa gcattatttt actatactgc atgcattttt tcaatatatt tataaaaaat
    14281 tcatataaat ttcatttatt aaatattttc acatcatata gttatacttt taataaataa
    14341 tagtaagatt tttaattagt cagctacgta tattttttat tgcaaagctt aaaataaacc
    14401 ttttcatata ttttcaaata aaaaagttgt cccaaaataa attttgaaac aacatcttac
    14461 tcaacagtat cttgattata taacactata atactctatt tatcttcata tccatttaca
    14521 acaacatcta attttccagt ttctggtgat ataattagac catgtacaat aataccatct
    14581 ggaattaaag gatgcccttt tattaaatca atactatctt ttacagattc ttctgcagaa
    14641 tcgaatccat gtaaccattg ttttgtatca attccagcac tacgaagtgt aagtattaca
    14701 tcattagata ttcctctatc taaaatttta ccaagtaact tatccgtatc aacattacac
    14761 ataccacatc catgatgccc tacaaccatg acctcatcta catcaaattc gtaaattgca
    14821 actaaaacac ttctcataat acttccaaaa ggatgcatta tagtagctcc tgcatttttt
    14881 attagtttca catctccatt ttttaaattc atagccttag gcaaaagctc agttaatctt
    14941 gtatccatac aagataagac tactattttt ttatttggat gttttgatgt cacatattgt
    15001 tcatattctt tgttctctac aaatgattta ttatactcta atatctcctc aagttttctc
    15061 atatgtaata tcaccctctt taaaaatttt tttattatta tatcactatt aaactacaat
    15121 atata
//
LOCUS       DAMJVM010000086        14763 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.090, whole genome shotgun sequence.
ACCESSION   DAMJVM010000086 DAMJVM010000000
VERSION     DAMJVM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14763
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.090"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            111..623
                     /locus_tag="PC523_002739"
     CDS             111..623
                     /locus_tag="PC523_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDF2476246.1"
                     /translation="MSDFKEILAEEIKSNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QVGFNIEHLNNTPYFKEAKMALICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKEA"
     gene            complement(777..1838)
                     /locus_tag="PC523_002740"
     CDS             complement(777..1838)
                     /locus_tag="PC523_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDF2476247.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGKKIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(1962..2855)
                     /locus_tag="PC523_002741"
     CDS             complement(1962..2855)
                     /locus_tag="PC523_002741"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDF2476248.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIENGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPTGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLVSALINKNFELLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIRENDDSFVDNMRNYLQNL
                     KSDWEIKELYCDSNGAVLNIL"
     gene            complement(2865..4346)
                     /locus_tag="PC523_002742"
     CDS             complement(2865..4346)
                     /locus_tag="PC523_002742"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDF2476249.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISNDGGLYVPSKFPNVKNELID
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVDNGEINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLNDKGVYKISEDMEKNLASFHGEYSNEEEVKNTINSVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     misc_binding    complement(4454..4684)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5470..6378
                     /gene="trxB"
                     /locus_tag="PC523_002743"
     CDS             5470..6378
                     /gene="trxB"
                     /locus_tag="PC523_002743"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDF2476250.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKIKIVKTSSAEHKAKA
                     VILANGVERRKLGCVGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARKNIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(6468..8291)
                     /gene="typA"
                     /locus_tag="PC523_002744"
     CDS             complement(6468..8291)
                     /gene="typA"
                     /locus_tag="PC523_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDF2476251.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEELSPLFKTIVNHVEAYPDYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            8503..8745
                     /locus_tag="PC523_002745"
     CDS             8503..8745
                     /locus_tag="PC523_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476252.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            8893..9084
                     /locus_tag="PC523_002746"
     CDS             8893..9084
                     /locus_tag="PC523_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HDF2476253.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            9375..11876
                     /locus_tag="PC523_002747"
     CDS             9375..11876
                     /locus_tag="PC523_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDF2476254.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYNPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMKNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKALFSLSPNMDSLVAIGTLAAFLYSLYTTIQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAVVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINNENILLGNRKLMNDNNIKLGDLEEKSNLLAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNVKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            12036..12704
                     /locus_tag="PC523_002748"
     CDS             12036..12704
                     /locus_tag="PC523_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2476255.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            12688..13965
                     /locus_tag="PC523_002749"
     CDS             12688..13965
                     /locus_tag="PC523_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDF2476256.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEVKADQERITKAINNIINNAIKYSPEESEII
                     IRLYDKNKRISKKNSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVIFTVEFKN"
     gene            14368..14589
                     /locus_tag="PC523_002750"
     CDS             14368..14589
                     /locus_tag="PC523_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476257.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
BASE COUNT         5638 a         1883 c         2279 g         4963 t
ORIGIN      
        1 aataatatat ttaaaaaaat ttattttatt ttcaaaacta gacttcattt ataatttatc
       61 acgaaaataa aaggaatata aatataagag aatgagaggt tataagagat atgagtgatt
      121 ttaaagagat tttggcagaa gagattaaaa gtaatccatt tggattgata ggtaaagatt
      181 ggacacttat aacagcagaa aatgaaggca aagtaaacac aatgactgct agctggggag
      241 ggcttggagt tatgtgggga aaagatgttg cttttgtagt aataagacca cagcgttata
      301 ctaaggaatt tatagataat acagataaat tttctcttac cttttttgat gaagattttc
      361 gtaaagagct ttcatattgt ggaaaagtat ctggtagaga agaagataag atatcacagg
      421 ttggatttaa tatagaacac ttaaataata caccttattt taaagaagcg aaaatggctc
      481 ttatatgtaa aaaaatgtat agtcagaaac tggaacctca atgttttatt gctgaaggta
      541 tagatggaag atggtatcca caaaaagatt atcacacttt atatatagcc gaaataacca
      601 atgtacttgt aaaagaagct tagttataaa ggttaatact atctctaatt aaataaaatc
      661 tctataaaag gaaattggca caaaaaagct aaggaagtaa atatgttgat acagattaat
      721 aaataacgta tttaaactcc ttagcttgat aaaattttaa tttagatttc tttaaattat
      781 aattcagaaa ttaattcatt gataacgtct tcaacagtgc ttaatttgtc aattttatag
      841 gcatcatttc cacaaaataa aagtgaattt tcaacatctc ctttaactgc attgattaga
      901 gcttgagaaa tacagtaagg tgtttctttt ggattacaag gaattaaaca attatagcac
      961 ttctttattt ctggacgatt tattttaact tcttctataa atttatttct tatggcacgt
     1021 ccaggaagac ctacaggact tttaactata tgaacatctt cttcactagc atttatgtat
     1081 gccattttaa agttctcatg ggcatcacat tcataagtag caacaaatct tgtaccaact
     1141 tgtacaccac ttgcacccat gtctatatat tttttaacat cactactaga agttatacca
     1201 ccagcagcaa ctactggtat ttttttacca aatttttctc cataagtatt tgcaactttt
     1261 aaaatttcaa caaattcctt atcatagtct atagaatcta tgttatcaag ttcctcgtta
     1321 gagtatccaa ggtgaccacc agcttttgga ccttcaacaa ctattaagtc agcagtagtt
     1381 ttctcttttc tatcccacat ttttaaaatt acattggcag cttttgcagt tgaaactata
     1441 ggtgctattt ttacattact tccctttact aatgatggta acttgttagg taatccagct
     1501 ccggatatta taaggtctac accagcttta acagcttcct ttacgtatgt ttcatattct
     1561 ttcatagcta ccataaaatt aataccaata ataccgccat ttgagatttc tttagcttta
     1621 gaaatatgtt tttttatagc tcttaaattt gcatttatag tattagtttc aaaatcttcc
     1681 tcatcatatc caatttgaac tccagaaatt acaccaacac caccaagctt agcaactgca
     1741 ccagctagtg atgacctaga tataccaacg cccattccac cttgaattat aggaactttt
     1801 gcaactaaat ctctaattat taactctttt atgataatcc ctcctcaatg taaacatgtt
     1861 taaagaacta gattataata cttaatatta ttacctagtc ctaattatta tactacaaat
     1921 tataataaaa gagtagttat attttgtata gaagacgata attataatat atttaaaaca
     1981 gctccattag aatcgcaata taactctttt atttcccaat cagattttaa gttttgtaga
     2041 taatttctca tattatctac aaaactatca tcattttctc ttattaaaga cattatagta
     2101 ggtccagctc cacttaaaaa tactccaata ctatttaatt gttcagattt ttcaacaata
     2161 tcattgtagt tttcaattag agttcctcta taatcttgat gcaatttgtc ttgacaagct
     2221 actttaagaa gttcaaagtt tttgtttatt aaagcagata ctagtaaagc aactctacta
     2281 acattgaata ccccatcaga atatggtatt tcctttggta aaacacctct agctttttct
     2341 gttgataatt tgaaattagg tatcattgca caaaatttaa gttcttcagg aatttttata
     2401 atatcatagt gtatattttc attatcagta acagatacaa tcatatttcc taaaatagca
     2461 ggagcaacat tatctgggtg accttctatt tctgaagcta tatttagtaa ttgattttta
     2521 tctaaattag cacctgatag agcatttgca gctataacgc cagctactat acaagaggca
     2581 ctactaccaa gacctctaca tataggcact tcactttgga tttttatttt gataccagta
     2641 ggtatttttt ctggtttaac tctatcgaaa aagtacttca tagatgtata aactaagtta
     2701 ttttcgtttt tgtaagcgtc ttcacaacct tctatttcaa gaccattttc aatttcttca
     2761 acataaaatt tattgtatat atttaacgca attccaagac aatcaaatcc tggacctata
     2821 ttagcacttg ttgcaggaac tataatctct aacataataa tcacctatac ctttaagaat
     2881 ttttttatag ctagttttaa ttcatctttt tcataaatat ctttatgtag tatttctgca
     2941 tttttaagat ttttaatagg ttttggtata tctattttag aaatacttga taatctatca
     3001 attatagcaa agtcatcaag gtctttaaaa tcacaatcta tagattttag aacatcttca
     3061 gaaaacttga atggacttgc agtacttaca ataacagttt ttgtgttatc agaagtttct
     3121 tttttatatt tttcataaca attatatgca acagcagtat gagtatctat caaataatta
     3181 taatttttaa acacgctgtt tatcgtgttt ttaacttctt cttcatttga atattctcca
     3241 tggaatgagg ctaaattttt ttccatatcc tcacttattt tgtaaactcc tttatcatta
     3301 aggtctgata gcagtttatt tacaatatca gtatttctat ttgatatttc aaacagtaat
     3361 cgctctaaat tacttgagat taatatatcc atagaaggag aagtagttaa tttcaatgtt
     3421 ctatttttat catatacacc agttttaaag aaatcataaa gaacattatt atcatttgat
     3481 gcacatatca atttgtttat tggaagaccc atttgtttgg cataatatcc agctagaata
     3541 ttaccaaaat taccagttgg gacaacaaaa tttattttat catttaaatt tatttcccca
     3601 ttatcaacta atttcatata tgaataaaaa tagtatgcaa cttgtggaag aagtcttcct
     3661 atattaattg aatttgcgga agataatata taattattat ctaataattt ttgattaaat
     3721 tctttatcag aaaaaatctt ctttacacct gattgtgcat catcaaaatt tccttttata
     3781 ccaactacat atgtattttt tccagtttga gttttcattt gaagtttttg tactggactt
     3841 acaccatctt ctgggaagaa tacaattatt tttattttat ctatatcttt aaatccctca
     3901 agtgcagctt tgccagtatc tccagatgta gcagtaagaa tcacaacatc tttctctaag
     3961 ttatctttct ttatagatgt tttaagaaga tgaggcatta tagtaagtgc catatcttta
     4021 aatgctaaag ttggaccatg gtacagttcc aagaaataag tatcatttac tttatttaac
     4081 ggagctatac tactgcaatc aaacttttca tcatatgcat tttctataca atttttaatt
     4141 tcatcttcag taaaatcaca taaaaattta ctaagtacaa aaaatgctat ctgagaatac
     4201 gttaaattta ctaaatctat aagttcattt tttacattgg gaaatttact tggaacataa
     4261 agaccaccat catttgatat tccttttatt attgcttgtg atgcagttac tttttcttcg
     4321 gtgcctcttg tacttgaata atacattttt tataaccctc catatatttt aaatttttaa
     4381 ttagctaatt aagttagata cagaatattt taaaaactga atttaggtaa taaaaaaaga
     4441 ccctcatccc taagaaaggg acgagaatct atttcccgcg gttccaccca aattgattta
     4501 aaaaaatcca cttgatactt taacgtaagt aataaaacgt ctattcctac taaaatcata
     4561 aagataactt cagatagaag ctcataggta gttttcaatc cttctaatta tggaaacatt
     4621 tcagctagta gtttccttct ctggtaatcg attcagattt actcttccta atcaatgcct
     4681 ttcaatattc ttaatattat taattattct agtttataaa acagacaaag tcaagtatta
     4741 atataaaaat ttggaaaaaa tattttttaa attgtaaaaa aattgtaatc aaattgagta
     4801 ttagaaaaat aattttttaa cttttttaaa agcttaaaaa cctagatttt catagtgatg
     4861 aagctgtttt tagtaaaagt aatcaaaaga taactattaa atatctgtaa tattacaatt
     4921 ttatattaca ctatattaca aaaaaagttt acaaaacttt aatttgaaaa atgtaattat
     4981 atggtatatc atataaatat aagtaaatag tgctaatatt taaactatta atcataattg
     5041 gttcaacaaa agtatatagt taataatgtt taatttgaag aaattgtcag tgtaaaatta
     5101 catatatatt caaattaaat tcataaatat ttcacataat aatagtatac tgtaaacaaa
     5161 aggaggtatt aaatatggga atagtactta taacagcagc attagttttg gttatgtatg
     5221 gactttcaat tttatcgtat atatcagaaa gcaatatatt agaagacact gtaaatgata
     5281 accaataagg ttttgaatta tataaataag tgagaactgt aaataaaagt taatttttat
     5341 ttgcagtttt tttatatata tgtaaaataa agttaattaa taaaaaataa tgttttattt
     5401 atagtatata tggataataa tagtaaagta taagagtaat gagaataata tataagagag
     5461 gtaaacgtta tggtagatat cattgtaatt ggtgctggac cagcaggttt aacttcagca
     5521 atttatgcta tgagagcagg gctaagtgtt acagtatttg aaaaaaatat ttatgggggt
     5581 caagttgcaa gtacctctga ggtagaaaat tacccagcag ttcagaagat ttctggggta
     5641 gaattttcta ataatatata caatcaagca gttgctcaag gtgttgatat tcagtttgat
     5701 gaggttgaag aaataaatct agagggaaaa ataaagattg taaaaacttc ttcagctgaa
     5761 cataaagcaa aggctgttat tcttgcaaat ggagttgaaa gacgtaaatt aggttgtgta
     5821 ggggaacaag aatttacagg tagaggagta tcttactgtg caacatgtga tggagcattt
     5881 tttaaagata aagaagttgc tatagtaggt ggaggaaata ctgcattaga agatgcttta
     5941 tttttagcca acaattgcac aaaggtgtat ttaattcaca gaagagatag ttttagaggt
     6001 gaagaagtgt tggaaaaatc tgtgaaagca agaaagaata ttgagatact ttacagtcat
     6061 ggtgtagaaa aaatagaagg agaaaagact gtatcgaaaa tagaggtaaa aaacttaaag
     6121 actgaagaaa aaagaactat agatgtcagt gggattttta ttgctatagg tcttaaacct
     6181 aataataaaa tgtttgaaaa tgttctcgat ttggatgaag gtggatatat aatttcagat
     6241 gaatcttgta caacatctgt ggaaggtgta tatgtagcag gagatagtag aactaagttt
     6301 ttacgtcaaa taataacagc agcatcagat ggagctattg cagctgttca agcagctaat
     6361 tatataaatg ttgaatagta taaatttcaa ataaaaatag ccatataaac tgaaataatt
     6421 tttcagtatt atatggctat tttgctaact agaggtaaat gtatgtttta tttaccttgt
     6481 ctagatttat tatatcttaa tctatcatgt tcatttaaga atctttttct aagtcttata
     6541 gaatcaggtg taatttcaac taattcatca tcttctataa attctagagc ttcttcaaga
     6601 gtaaatattc taggaggaga taattttata gcatcatctg aaccagaagc acgtacgtta
     6661 ctcattttct tgtttttaca agggttgact accatatcat cttttctaga attcattcca
     6721 attatcatac cttcgtaaac ttctacacca gggtctacga acattacagc tctatcactt
     6781 aaggcattaa gagaatatcc catagtaaca ccaggtcctt gtgctattaa aacaccattt
     6841 cctcttgaag gtatttctcc tttaaattct tcaaattttt caaatgaacg aactaaagta
     6901 ccttctccac gagtagcatt tataaattca cttcgatatc ctaaaagacc acgagtaggt
     6961 gctaagaact ctatttttac atagtctcct tctattgaca tagactccat catacctttt
     7021 ctcatgttta attcatttat aatagtacca gagtaaactt caggacagtt tacaacaact
     7081 ctttctattg gttccattag ttttccatct tctttatgca ttaaaacttc aggcttagaa
     7141 acaccaactt cataaccttc acgtctcata ttttcaagta atatagataa gtgaagctct
     7201 ccacgtccag aaactttata tccatcagta gtatcaagag gttcaacttt aagaccaaca
     7261 tttacttcaa gttccttttc aagtctatct ttcaaatgtc tagttgtaac gaattttcca
     7321 ctttttccaa caaatggaga gtcatttact aagaagttca tagaaagagt aggctcttct
     7381 atttttatca tttccatagg tagtggacta tctaaatcgc atatagtctc acctatagag
     7441 atatcaggta taccagctat aacaactatt tctccactag tagcttcatc aacctcaact
     7501 tgttttaaac cttcatacac agaaagtttt gatacttttc ccttagatac aacagaatct
     7561 tctctacata tagcaacttg tgtattattt ttaagagtac ctttgtaaat tctacctata
     7621 ccaagtctac ctacataatc atcatatgca agagctgata tttggaattg tagaggttca
     7681 ttatcgtaat caggataagc ttcaacgtga tttacaatag ttttgaatag aggagaaagt
     7741 tcttcgctat catcatccat ttccagttta gcaatacctt gtttagcaat accatatatt
     7801 ataggaaatt cgcattgctc atctgttgca tttaagtcta caaataaatc gaaaacttca
     7861 ttaactactt cttccgctct ttggtctttt ttatcaatct tgtttataaa taatattggt
     7921 tttaaaccga actctaaaga tttttgtaaa acgaatctag tttgaggcat tgggccttca
     7981 ctagcatcaa ctaatagaat aactgtgtca acagttttca taacacgttc aacttcagaa
     8041 gaaaagtcac tgtgtcctgg agtatcaact atgtttattt tgtaatcctc atagtttatt
     8101 gcacagtttt ttgaatatat agttatgcct ctttcttttt caaggtcgtt actatccata
     8161 acgcagtctt taactacttc gttctttcta aaaacaccac tttgagaaag gaaagcatca
     8221 accaatgttg attttccagc gtcaacgtgg gctataactg cgatatttat aattttatgt
     8281 ttttgtgaca tttttgttgc tcccttcgat taaaataact tattaattat agcacaatat
     8341 gttattaggt acaaggagga atatattaaa ttgtattaat atagttcaaa ttggataaaa
     8401 atgttagttt tatatccgaa aattattctg tttaaaaaaa ttttttagac aaataataac
     8461 cttaatagat gaaaagatta ataaaagagt aaaggatgat atatgttgga agtaaccaaa
     8521 ataataatta aatattttgc aagtgaaagt gatacttttg taaaagaaga agtttgcaat
     8581 tttgaagaac ttcttaaatg gtctcataaa tttattgaag gtaaaaagat agaagatgtt
     8641 ttgtatataa aatctaaaga aaactataaa gtaaatgtaa ttaagtcttt agaaattaag
     8701 gaattggaaa tagtgagaaa acaacagata ccaccgtaca tgtagtatta ataatatata
     8761 aaattaatgt gtaatactta tattaggata aatatggtat gtatggtaac aatttatata
     8821 aaaaaaactt ttataaagtt tatatttggg tataaaagta ttaattagac attaattagg
     8881 aggaacttaa atatgataag agtatgtcca aactgttcaa atgtggatgt aaataagcta
     8941 aaaactttgg ttggagaaga aaatgtaaaa actggttgta taggtcagtg tagagcattc
     9001 aaaaaagaag ctgttggaat aatagacaat gagcttgaaa taaaggaaac agaagcggag
     9061 ttttttgaag cttgtaaaga ataaatttta aataaaatat aaattataat aaatagagcg
     9121 tataaattgt ataaatatta aacaatttat atgctctatt tttatgtata tatttaatta
     9181 taagtatact tttttattaa atacaaataa atataactaa tacatttatt tataaaaaaa
     9241 agattgattt aatataccta cagggggtat aataaaaata tagatttcta aataaataat
     9301 ttatcaatac tacatatcag aataaaacgt tacaaattag ttgaaaatat gttgaaagaa
     9361 aggaagattt tattatgagt tcaaataaag tggtagaaag ttacaaaatt acaggtatga
     9421 catgtgcagc ttgtgcaaag gcagtagaaa gagtaactaa gaagatggat ggagtgtatg
     9481 accaaagtgt aaatatagct actgaaaaac ttaaaataga atatgataat tctaaggtta
     9541 attttgatga tataaagcaa gttgtagaaa aagcaggata tggaattata aaggaagaaa
     9601 gtaataaaaa aattgatatg aaaattgatg gaatgacatg tgcagcttgt gcaaaagctg
     9661 tagagagagt ggtaaaaaaa ttggatggag tagaaagtat tagtgtgaac attgcaacag
     9721 ataaagctaa tatagactat aatccatcaa aagtaaagtt atctcaaata aaagcagcta
     9781 ttgaaaaagc tggatataaa ccaatagaag aggtaagaaa taaggttgat gtggatgaag
     9841 ataaattgag aaaagaaagg gaaatgaagt ctttatttgt taaatttata gttgcaatag
     9901 tatttgcagt acctttgttt tatatagcaa tgggacctat gataataaag cctatagggc
     9961 catggccttt gccagaaatt ataaatccaa tgaaaaatac ttttaactac gcattaattc
    10021 agttaatttt agtaatacca gtaatgatag ctggttataa attttatata aatggattta
    10081 aagcactttt ttctttaagc cctaatatgg attctttagt tgcaatagga acattagcag
    10141 catttttgta tagtttatac acaacaatac aaatagcaaa tggtcaaatt caaggtatgc
    10201 atcatcatca gttatattat gaaagtgcag gaataataat agcacttatc ttacttggta
    10261 aatatttaga atcaaaatca aaaggaaaaa catcagaagc aattaaaaag cttatggggc
    10321 tacaacctaa aactgcagta gtgttggttg atggtaaaga agttgaaact cctatagaag
    10381 aggtagaaat aggagatata ttattagtaa aacctggaac aaaaatacct gtagatggag
    10441 ttgtaataga aggatataca tcagttgatg aatcaatgct tacaggtgaa agcataccag
    10501 tagaaaagaa tgttggaagt aaagtgacag gagctagtat taataaaaat ggtgttatta
    10561 agtttaaggc tgaaaaaatt ggtggagata ctgctcttgc ccaaataata aagttagttg
    10621 aagatgcaca aggtacaaaa gcacctattg ctaaacttgc ggatactgta tctggatatt
    10681 ttgtaccaat agtaattgca atagctgtag ttgcatcatt attgtggttt ttaataggag
    10741 gaaaagatat agtatttgta cttactatat ttatatcagt acttgtaatt gcttgtcctt
    10801 gtgcattagg attagcaacg ccaactgcta taatggttgg aactggaaag ggagcagaaa
    10861 atggaatctt gataaaaggt ggagaggcac tagaatctgc acataaagta aatacagtaa
    10921 tatttgataa aacaggtact ataacagaag gaaagccaaa agtaacagat atagtattaa
    10981 ataataatgt aaaagaagag tatcttatca agattgcttc aagtgcagaa aaaggttcag
    11041 aacatcctct tggagaagca atagttaagt atggagaaga gaagaatata aaatttgaaa
    11101 aagttgacaa ttttaaagct ataccaggtg caggtataca agttactatt aataatgaaa
    11161 atatattact tggaaataga aagcttatga atgacaataa tataaagctt ggggacttag
    11221 aagaaaaatc aaatttattg gcaagtcaag gtaaaacacc tatgtatata gctgtagatg
    11281 gaaatttatc aggtataata gcagttgcag atgtagttaa agaaagtagt aaaaaagcaa
    11341 tagaaatact tcatgatatg ggaataaaag ttgcaatggt aactggagat aatgtgaaaa
    11401 ctgcaaatgc tatagcaaat caagtaggta tagatatggt tttagcagag gttttaccag
    11461 aagataagtc aaaagaagtt gaaaaactac aaaatcaagg caagtttgtt gcaatggtag
    11521 gagatggaat aaatgatgcc ccagcccttg caaaagcgga catagggata gcaataggaa
    11581 gtggaacgga tgtagctatt gagtctgcgg atatagtact tatgaagagt gatttaattg
    11641 atgttccaac agcaattaaa cttagtcatg aaactataaa aaatataaaa caaaacctat
    11701 tttgggcatt tggatataat acgattggta tacctgtagc agcaggaata ttgtatgtat
    11761 ttggaggacc tctgttaaat ccaatgattg cagcagcagc catgagctta agttcagttt
    11821 cagtcgtatc aaatgcactt agacttaaaa acttcaaggc atacaagaga gactagatta
    11881 acaagttaaa aagaatgtat ccaaaagagc tatctttaaa ttaaaaggat taatttacaa
    11941 attaagatag ctcttttttc atgtaaaatt cacaacaata agataaaata taagtaatat
    12001 atgaaatttt aaataaaata gaaaggattg atagaatgaa ttcgtcaata cttgtaattg
    12061 aagatgattc aaatatacaa gaattaatat cagaattttt aagtgcagaa ggttatcaag
    12121 tggatacagc aaatgatggg ttggaaggta tacaaaagtt taagcaagga agttatgatt
    12181 tagtaatatt agatataatg atgccaaatt tagatggtta tggagtatgt aaaatgataa
    12241 ggaagtcatc aagcgttcca attatatttt taactgcttt aaatgatgaa ggagaccaac
    12301 ttaaagggtt tgacttagaa tgcgatgact atataacaaa gccattttca tttaatcttc
    12361 ttataaaaag agtagaggca attttaaggc gaagtaacaa gactataaat gacaaattta
    12421 tagtttttga aaaattaaaa ttagatttaa acacatatat tgcagaaata gatggagaac
    12481 ctatagaact tacactaaaa gagtttaata tattaaaagc tttgatagaa aagtatccac
    12541 aggttataac tagagaaggt cttttggata gtatatgggg ttatgactat tatggagata
    12601 ctaggattgt tgatgcacat ataaaaaata taagaaaaaa aatatcatta ccatatatta
    12661 aaactgtaaa aggaatagga tatacacttg agaaggatat ttgataagtg ggaaaagtta
    12721 agtataaagt ataagttatt ttcaataacg acatcattat taatagcatt agctttaata
    12781 atatatctaa ttttatattt tttattgcca tcatattatc atgagtataa aattgagtct
    12841 cttcaagaaa gtttaaagtc tttagtggat agttcaattc atttcgatac atatactttg
    12901 gaagaaagac tttattatat ggctaaagac caaaatttgg ctattttatt aaaagataac
    12961 caagggaaaa ttgtatatgg taagaatgaa gtagttattt taagatatag taagtacatg
    13021 attaatagtc ttgaggatga gtataggact tctataccta tatatactaa agatgcaaaa
    13081 gatgggccat atactttaga gctcgttatg ccattacaac caattgacga agcaaacgaa
    13141 gttataagaa aactgatgcc gtacattata tcaatagcaa tattgatagc gattattggg
    13201 gcatatatat attcaattgt aataacaaaa ccgcttataa atataataga gagtgagaga
    13261 gagcaggaat atagaaggaa ggattttgtc gctacaatat cacatgaact aaaaactcca
    13321 ataaccataa taagtggtca aattgaaggt atgatttaca gtgtaggtaa gtataaggat
    13381 agggatactt atctaaaaaa atcgtatgaa tgtacccaag aattaaaaga tttagtaaat
    13441 gaaatgatag aggtttcaaa atcagaaata cttgaaaaag atttgaaatt ggtatcaata
    13501 aatataagtg aattattaaa tagacttgta aaaagacaag tatttttaat agaagaaaaa
    13561 catatgaaaa ctattttaaa aatagaagaa aatttagaag ttaaggcaga ccaagagaga
    13621 ataactaagg caatcaataa cataataaat aatgccataa aatattctcc tgaagaatca
    13681 gagattataa ttagactcta tgataaaaat aaacgaataa gtaaaaagaa ctctaatcaa
    13741 agagtggttt tagagattga aaatactggt gtaactatcg agaaacgtta tttagaagag
    13801 atatttaatc cattttatag aatagaaaaa tctcgttcaa gaaaaactgg aggaagtgga
    13861 ctgggacttt atatagtcag ccaaattttt aaaagtcatg ggtttgatta tagcataaaa
    13921 aacaaagaaa actctgtaat attcacggtc gaatttaaaa attaattact tattataaag
    13981 aatatataac tttcaataat agttcatttt aaaatcacaa aaaattcata ctgaaactgt
    14041 attatataaa tataaagaag ctttaaacca taaaatacac caactataat gatgagattt
    14101 ccttacatcc tccatagaag tcatcattat agtaacccaa caattaagtt actatgaatt
    14161 taaaaaataa caccagacat ataatctatg tttagtgtta tttttatttt gaaataaaaa
    14221 atttctaatt tattgaaatg tatagattta gatagtctta aactatattt aagggcttaa
    14281 aaattataat tgatattaca aataaatata tatactttgg gaaaaataaa tatagaaagt
    14341 attatattta aagaggaggt agttttgatg gacagattaa aaagagatat gaaaaatatg
    14401 aaagaagatt taaaagaagg agttaaaaag acagcttatg ttatgaaaaa tacagctgaa
    14461 gatttgaaag atgatgcaca aggtgctatg gtaaacttag aaatgaaaaa agatgagatg
    14521 atggaaagat atgaacaaaa gaaattttct aaagaattag aaaagcaaat gaagaatgaa
    14581 atgaaataag tttttattta tgaataacaa attattgtgt tcatataaaa agtagctagt
    14641 aatattaaat agtttattta aggaaataga ctttctaaaa ataactgtat tagatttaga
    14701 aagtctattt caaattagtt ttatgaatag acataaggat aaaatcgata ttatagaact
    14761 aaa
//
LOCUS       DAMJVM010000087        14571 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.091, whole genome shotgun sequence.
ACCESSION   DAMJVM010000087 DAMJVM010000000
VERSION     DAMJVM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14571
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.091"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(101..760)
                     /gene="deoC"
                     /locus_tag="PC523_002751"
     CDS             complement(101..760)
                     /gene="deoC"
                     /locus_tag="PC523_002751"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDF2476258.1"
                     /translation="MKHILQTVDHTMLKATTTLKDIKTLCDEAVRMNVASVCIPPSYV
                     KSATEYLNGRVNICTVIGFPLGYQTTKVKVFEAKDAIENGAVEIDMVVNISDIKNEEY
                     EKIENEIKEIKSVIGDKVLKVIIETCYLNEYEKIKMCEIVTNSGADFIKTSTGMGTNG
                     ATLEDIKLMKKHIGEGVKIKAAGGIKSISDAEKFIEAGAQRLGTSSICKILNGEKPME
                     Y"
     gene            complement(787..2331)
                     /gene="murJ"
                     /locus_tag="PC523_002752"
     CDS             complement(787..2331)
                     /gene="murJ"
                     /locus_tag="PC523_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDF2476259.1"
                     /translation="MSRVARAAFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDINSKKGEKEALKFLNNVLNIIVGICIIVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTRILIIGIIFIGITSVMSAFLQIKENFIVVGF
                     GSIPYNIVIIISIVLSTVLGPYVLPVGAVVAMVVQLFFYLFFVNKTKYKYLFYLDFKD
                     ESLIKLLALLSPVFIGVAVNQVNSLVDTTLASTLIKGSIPALTYADRLNGFVTGTFTA
                     SIVSVMYPTLSKLSAENNQKKFTISVKSSVNMIIISMIPISVASIVFAKPVVRIIFER
                     GAFDSRATQMTATALVFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIVSVGVNILL
                     DLALIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIRAIFKSLVSSCVM
                     SFIAYFIYKFIFGILGLGTLNELISLTISVVIGFSVYTLLMILFKVEEVDMILNIAKR
                     KLHLKK"
     gene            complement(2626..3390)
                     /locus_tag="PC523_002753"
     CDS             complement(2626..3390)
                     /locus_tag="PC523_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476260.1"
                     /translation="MKNINKECLIKLLTVMVVLIQIIYLLWKKEIVYYIHPRMIPYLV
                     ISICILLFIALSYIMILTKPKHIYQYGSTKYIILSFLITTLLISYSGLNIDKEKTKVE
                     KYKGKEKYQDKIYEKEEFLDRTLEERIYDEKFDVFDTEIYDFLNLLYTKQSVLKGKEV
                     TYVVFVHYDDSLEKNQFIASRSIMVCCAADMISSGPIVNMNQPPKWKEGEWILLTGKI
                     EYKEQKNSSNKTAFLNYIDAKPIEKPLEEVVYPNFG"
     gene            complement(3531..5087)
                     /locus_tag="PC523_002754"
     CDS             complement(3531..5087)
                     /locus_tag="PC523_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF2476261.1"
                     /translation="MSTRIDIVAGFLESGKTKFINEYLRNKQYDDKDNILLIVLEEGF
                     TEYSIPFSSKNYIKIERFKDINCLTKEYLKKCSNLYNYILIEYNGTWNIEEFLDIKLP
                     YSCYLGKIYCLVNADTFDVYFRNIDSMVEQIINSDEFYISGSNSIEKTEKITSYIRAL
                     QPSADIFYIDESGNYKKIFPLSLKLKNLIYRIIKFVIVILFFLLLLGVKIQIIKVQDI
                     NDFNRMFFGIILQAIPFLLIGIVLSSCIQFLVSEEKFIYFASRHKVLSYPIFLFMGCI
                     LPLCDCAMVPITEKFIQKGLKVSHAMTFFCISSSINPIVILATIYAFPENNKVIWIRI
                     LVSIMLSALVGISFYFVEKNKNFSPYKKNKIPLFNNIEYEITKITKKGKKFRFLALGI
                     HIGNEFLRIMMFIIIGAFISTIAQILKMNPAFSGYFTNDYAFLYMFFASVFLSICASS
                     NAFIAKGFTNSLPLMSVIPFMVFGPLLDIKNLIVMSSRFSKKFMTIFIIILLVCYIMY
                     FVFFNMEIKL"
     gene            complement(5088..6017)
                     /locus_tag="PC523_002755"
     CDS             complement(5088..6017)
                     /locus_tag="PC523_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein P47K"
                     /protein_id="HDF2476262.1"
                     /translation="MIKIDIISGFLGSGKTTFIKKLLSAYYKDKKVVLIENEYGEVGV
                     DGLLFKNENIQVRELYSGCICCSLNTDFTKSLKEVIELFKPDTVIVEPSGVGKLSEVI
                     QNCIPLVKQNLVSINHKFTIVDVMKYSVYLKNFKEFYDDQIKYCDAVLLSRVDVCDEI
                     KVREVISSIQNKYHKKYIVSLEQNLLDETIVNYIMSTKSVVHSENDMHCNCHHNHVVH
                     NADDVFSSWGIKLEKSYKYEYIKEILEQFNSGSFGNIIRAKGIFKCEDGLFYEFSFVP
                     DEIKVIPCNYISSGCICVVGFKPNKEKLERAFV"
     gene            complement(6073..6861)
                     /locus_tag="PC523_002756"
     CDS             complement(6073..6861)
                     /locus_tag="PC523_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDF2476263.1"
                     /translation="MLSYGFMQRALFVGLCVSIAIPCVGLILVLRKFSMLGDALSHTA
                     LAGVCFGLVVGISPTIGAIMSCIIASFSVSIIGKRMKHNEEIAIAVILSLGVGISGIL
                     SNYIKSSVNINSFLFGSIISISGREFALVIALTILILIIFFKYYYEFMFYAFNEKEAQ
                     KHYVNTRKIDILFTILIGLTISISARVVGALIISSLLVLPVACGLILGKSYKSTLLIA
                     IFLSMLFVLSGLTISYYIGLSPGGAIVILGSITLMIILLYKKRV"
     gene            complement(6854..7498)
                     /locus_tag="PC523_002757"
     CDS             complement(6854..7498)
                     /locus_tag="PC523_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2476264.1"
                     /translation="MKNIVEIKDGAFSYTDCNVFKSVNVNINKNELVGVYGENGSGKS
                     TLFKIITKEVKLQSGEIFTFDSLTTSFLGQNDRVMAEGSPLTLMEFLLMYQHKEKSKL
                     FKNKKNVMMLLRKYNMEHYAKKQLKSLSGGQLQKSMILKSIMNHPDIILFDEPLNALD
                     EVNKISVMDMIMDLHNNGHTILLILHDYEQLKKIADKILICENHTIKCEVLNYA"
     gene            complement(7501..8403)
                     /locus_tag="PC523_002758"
     CDS             complement(7501..8403)
                     /locus_tag="PC523_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDF2476265.1"
                     /translation="MKRFVLLFCCSLLLLTGCSFKDVKKTSSNKKLSVYTSFYPMYDF
                     AKKIGGTKVDVKNMTPAGIEPHDFEPTTNDIKGLSNADMFIYNGDNMEHWVNDVIQSL
                     DNRNILIVKASDGIDLMKGEKSSDSMDPHTWLSIRNAKKELEKIKNAFCKKDPDNATY
                     YEKNYYKYASEFDALDSQYKKELLPYKGRSIVVAHQAFGYLCKDYNLKQIAIEGLSPD
                     SEPSPARMAEIIKSAKKNKIKVIFYEELVNPKVAETIAKETGSETKVLNTLEELSDKK
                     IKEGEDYISVMKTNLQNLVYALSK"
     gene            8538..8969
                     /locus_tag="PC523_002759"
     CDS             8538..8969
                     /locus_tag="PC523_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2476266.1"
                     /translation="MVVLYMNNYLKKHNLKSTEKREAVIKVLENSNVPMTIESIHEKA
                     NTIVEMNLSTIYRTITTLCEKNITVLVAIHDGKSYYQLNKHRHEHVLICKECKDIIII
                     DECPLHELEHKLEDETGFDIIEHKLEFIGICPKCKRTNSRT"
     gene            complement(9444..10328)
                     /locus_tag="PC523_002760"
     CDS             complement(9444..10328)
                     /locus_tag="PC523_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2476267.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVMHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGRVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(10619..11095)
                     /locus_tag="PC523_002761"
     CDS             complement(10619..11095)
                     /locus_tag="PC523_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HDF2476268.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            11361..12536
                     /locus_tag="PC523_002762"
     CDS             11361..12536
                     /locus_tag="PC523_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HDF2476269.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQDYDYNSMKNTLDNVSDNVNEQLKKLGESVKSSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     SDSDTNNSKNNTSTNNENKDNSQTNENDTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(12617..13420)
                     /gene="proC"
                     /locus_tag="PC523_002763"
     CDS             complement(12617..13420)
                     /gene="proC"
                     /locus_tag="PC523_002763"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDF2476270.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQETLDRI
                     YANFGINTTKDSKEVTRSSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIVGDDKKIVRTMPNTPALVNEAMSSLSINKNINDEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(13460..14044)
                     /locus_tag="PC523_002764"
     CDS             complement(13460..14044)
                     /locus_tag="PC523_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2476271.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVAKILVVSVPNPFEKSNEVKEA"
BASE COUNT         5040 a         2443 c         1559 g         5529 t
ORIGIN      
        1 atctataata ctctatttaa aaattcactt tttttattat atctatttat aatcttttaa
       61 gttactactt gttatacagc tactagtata tataaaatat ttaatattcc attggttttt
      121 cgccatttaa tattttacat attgaacttg tacctaatct ttgtgcacct gcttctatga
      181 atttttctgc atcagatatt gattttatcc ccccagcagc ctttatctta acaccttcac
      241 caatgtgctt tttcataagt tttatatctt ctagagttgc tccatttgtt cccataccag
      301 ttgatgtctt tataaagtct gcacctgaat ttgttacgat ttcacacatc tttatttttt
      361 catattcatt cagataacac gtctctataa ttacttttaa taccttatct ccaatgacac
      421 tttttatttc ctttatttca ttttctattt tttcatattc ctcatttttt atatcagaaa
      481 tgtttacaac catatcaatt tcaactgctc cattttcaat agcatctttt gcctcaaata
      541 cctttacttt agtagtttga taccctaaag gaaaacctat tactgtgcaa atatttactc
      601 ttccatttaa atactcagta gcacttttta catatgatgg tggtatacaa actgatgcta
      661 cattcattct tacagcttca tcacacaaag ttttaatgtc tttcaatgta gttgttgcct
      721 ttaacatagt atggtctact gtctgtaata tatgtttcat aaaaacctcc taagtgtata
      781 attaagttac ttttttaaat gcaatttcct tttggcaatg tttaatatca tgtcaacctc
      841 ttcaacttta aaaagaatca tcagtaatgt ataaactgag aatccaatta ctactgaaat
      901 cgtcagagat attaattcat taagtgtccc taaacccaat ataccaaaaa taaatttata
      961 aataaaatat gctatgaaac tcataacaca tgaagatact aatgatttaa aaattgctct
     1021 tattatttta tcttgcccaa aatatcctac ttttctcttt aagtttaaaa aaagtaacaa
     1081 tatacatgct attgatgatg agctagttgc caacgcaaga cctccatggg ccataggttt
     1141 tatcaaagct aaatccaata atatattaac tccaactgat acaataccat ttaccatagg
     1201 tgtttttgta tcctgtaaag aataaaaaac ttttcctaat atgtctctta atccaaaagc
     1261 tgtcatccca actgcataaa aaactaaagc agtagctgtc atttgagtag ctctagaatc
     1321 aaatgctcct ctttcaaata taattctaac aacaggctta gcaaatacaa tagatgcaac
     1381 agaaattgga atcatagaaa ttattatcat atttacacta gacttaaccg aaattgtaaa
     1441 ctttttttgg ttattttctg cacttaattt cgataacgta ggatacatta cagatactat
     1501 tgaagctgta aatgttcctg taacaaaccc atttaaccta tctgcataag taagtgctgg
     1561 aatactcccc ttaatcagtg tagatgctaa tgttgtatct accagcgaat ttacttgatt
     1621 tacagctacg cctataaata caggtgataa tagagccagt agctttatta aactttcatc
     1681 tttaaagtcc aaataaaata aatatttata ttttgtttta ttcacaaaaa acagataaaa
     1741 aaatagctga actaccatag ctactacagc cccaacagga agtacatatg gtcctaacac
     1801 agtacttaat actattgaaa taattattac aatattataa ggtatactac caaacccaac
     1861 tacaataaaa ttttctttta tttgtaaaaa agcagacata acactggtaa tccctataaa
     1921 tattatccca attataagta ttctagtgaa ttttacagta agtaaaaatc tttctccctc
     1981 aaatcctatg gcaaatatac ttacaagttg ttttgaaaaa ataactccta atattgctac
     2041 aattatgcat ataccaacta ttatatttaa aacattgttt aaaaacttta atgcctcttt
     2101 ttctcctttt ttactattta tatcttgata cataggaata aaggtagtaa ctatagcagt
     2161 tcctattgct gcaaatataa tatttggtat attcatagct gttaaataac tttctgtata
     2221 caagccagtc ccataaatcg aagataacac tagttctcta cccattccaa gaattttaga
     2281 tattatagtg actatcatta aataaaaagc tgctctagct actctgctca ttttatcact
     2341 ccactcttct cgcgtttctt cataattttt ttagaagaaa agtacttata tcagctatat
     2401 ttctaaatac cattttttat tttttatcaa tcaataattt taaaagtagt ggacacagaa
     2461 aatcactact tattttcaaa gtatattcta tcatatattg ttcattatga aaacttttta
     2521 tcttttgtct tatttcttta ggtattttaa atatataaag actagaaatt ttactccatg
     2581 tgaaacttat aagcaataac aatgtttaca tggagtaaat aaaaactacc caaaatttgg
     2641 ataaacaact tcttctaatg gtttttcaat aggttttgca tctatataat tgagaaatgc
     2701 agtcttatta ctagaatttt tttgttcctt atattcaatt ttacctgtta atagaatcca
     2761 ttctccctct ttccatttag gaggttgatt catattgaca attggaccac tcgaaatcat
     2821 gtcagctgca cagcatacca taattgaacg tgaagctata aactgatttt tttcaagaga
     2881 atcatcataa tgaacaaata ctacatatgt tacttctttt cccttaagaa ctgactgttt
     2941 tgtatatagt aaattgagaa aatcatatat ttctgtatca aatacgtcaa atttttcatc
     3001 atatatccgt tcttctaatg ttctatctag aaattcttct ttttcatata ttttatcttg
     3061 atatttttct tttcccttat acttttcaac tttcgttttt tctttatcta tattcaaacc
     3121 actatacgat attagtaatg ttgttattaa aaatgataaa ataatatatt tagtacttcc
     3181 atactgataa atatgcttag gttttgttag tatcatgatg taagaaagtg ctatgaatag
     3241 taatatacaa attgaaatta ctaaatatgg aatcatcctt gggtgtatat aatacacaat
     3301 ttcttttttc cataataaat atataatctg aattaatact accataactg ttaataattt
     3361 tatcaaacac tctttattta tatttttcat aatcaatctt accttttctg aaccctccct
     3421 tgactaaagt cgcaaggttc ctgcttcata aaattttaca taatcaaact ttaagataac
     3481 tgacgtaaat aataaaattt attctttact ataaaaagat aaatgctaaa ttataatttt
     3541 atttccatat tgaaaaaaac aaaatacata atgtagcaaa caagtagaat tataataaaa
     3601 atggtcataa attttttgct gaatcttgat gacattacaa ttaaattctt aatatccaac
     3661 aaaggaccaa ataccataaa tggtattacg ctcataagtg gtagagaatt agtaaatccc
     3721 ttagcaataa aagcattact tgaagcacat atagaaagaa aaacagatgc aaagaacata
     3781 tacaaaaatg catagtcatt agtgaaataa cctgaaaatg ctggattcat tttaagaatt
     3841 tgtgcaatag tagaaataaa tgcgccaata attataaaca tcattattct taaaaattca
     3901 tttcctatat gaataccaag tgcaaggaat ctaaacttct tacctttttt agtaattttt
     3961 gtaatttcat attcaatatt attaaataaa ggtattttat tttttttata tggagaaaag
     4021 tttttatttt tttcaacaaa ataaaaactt attcctacaa gagcagaaag cataatacta
     4081 actaatattc ttatccatat taccttatta ttttcaggaa aagcataaat tgttgccaat
     4141 ataacaatag gattaatact tgatgatata cagaaaaaag tcatagcatg tgatactttt
     4201 aaacctttct gtataaactt ttctgtaatt ggaaccattg cacaatcaca taatggtaaa
     4261 atacaaccca taaataaaaa aattggataa gataatactt tatgtctact agcaaaatat
     4321 ataaacttct cttccgaaac aagaaattgt atacaagaag aaaggacaat cccaattaaa
     4381 agaaatggta tagcctgaag tattatgcca aagaacattc tattaaaatc attgatatct
     4441 tgtactttaa taatttgtat tttgaccccc aataataata aaaaaaacag tataactata
     4501 acaaacttaa taattctata aataagattt ttaagtttta aacttagagg aaaaattttt
     4561 ttgtaattac cagattcatc aatataaaaa atatccgcag aaggttgtag tgctcttata
     4621 tacgatgtaa ttttttctgt tttttcaatt gaattacttc cactaatata aaattcatca
     4681 ctatttatta tctgctcaac catggaatct atatttctaa aatacacatc aaatgtatct
     4741 gcattgacta aacagtatat cttccctaaa taacatgaat atggaagctt tatatcaaga
     4801 aactcctcta tattccatgt cccattgtat tcaatcaaaa tgtaattata cagattacta
     4861 catttcttaa gatattcttt tgtcaaacaa ttaatatctt taaatcgctc tatctttata
     4921 tagtttttcg aagaaaatgg aattgaatat tcagtaaaac cttcctctaa tacaattaat
     4981 aaaatattat ctttatcatc atattgttta ttccttaaat attcattaat aaacttagtc
     5041 tttccagatt ctaagaatcc agctactata tcaattctag tactcattta aacaaaagct
     5101 ctttctaact tttctttatt tggtttaaag ccaactacac aaatacaacc actacttatg
     5161 taattacaag gtataacctt tatttcgtca ggcacaaagc taaactcgta aaacaaacca
     5221 tcttcacatt taaatatgcc ctttgctcta ataatatttc caaaactacc actattgaac
     5281 tgttctaaaa tctcttttat atattcatac ttatagcttt tttctagttt aataccccaa
     5341 ctactaaata catcatctgc attatgtact acatgattat ggtgacagtt acaatgcata
     5401 tcattctcac tatgtactac agattttgta ctcataatat aattaacaat agtctcatct
     5461 aataaattct gctctaaaga tacaatatat tttttatgat acttgttttg tatggaactt
     5521 ataacttccc ttacttttat ttcatcacat acatcaactc tagataacaa aacagcatca
     5581 caatatttaa tctggtcatc ataaaactct ttaaagtttt ttaaataaac actatatttc
     5641 attacatcaa ctattgtaaa cttgtgattt atactcacta aattttgttt aactaatgga
     5701 atacaatttt gtattacttc actcagtttt ccaactccag atggttctac aattactgtg
     5761 tctggcttaa ataattctat aacctctttt aatgatttcg taaaatccgt atttaaagaa
     5821 cagcaaatac aaccagaata gagttctctt acttgaatat tttcattttt aaacagtagt
     5881 ccatcaactc caacctctcc atattcattt tcaataagta ctactttttt atctttataa
     5941 taagcactta gtaacttttt aataaaagtt gtcttaccag agcctagaaa tcctgatata
     6001 atatcaattt ttatcatttg cacctctccc ttttattttt tatttgttct taccttagtc
     6061 taacgttttt atttagactc tctttttgta cagaagaata atcattaatg ttatgctacc
     6121 taaaataaca atagctcctc ctggactaag tcctatataa tatgaaatag tcaatcctga
     6181 taatacgaac aacattgata agaaaattgc aataagtaat gttgatttat aactttttcc
     6241 tagtattaat ccacatgcca ctgggagtac taataatgaa gaaataatca aagctccaac
     6301 aactcttgca gaaattgaaa tagtaagccc aattaaaatt gtgaataata tatctatttt
     6361 tctagtattt acataatgtt tttgtgcttc tttttcattg aaagcataaa acataaactc
     6421 ataataatac ttaaaaaata taatcaaaat caatatggtc aaggcaatta ctaaagcaaa
     6481 ctctctccca ctaattgata taatactccc aaataaaaaa ctatttatat ttacactaga
     6541 ttttatgtag tttgatagta ttccactaat tcctacacct aatgataaaa taacagcaat
     6601 agctatttct tcattatgtt tcattctttt acctataata gaaactgaaa aagaggctat
     6661 tatacaactc ataattgctc ctattgtagg actaatgcca accacaagac caaagcatac
     6721 acctgcaagt gctgtatgtg acaatgcatc tcccaacatt gaaaattttc ttaataccaa
     6781 aattaaacct acacagggaa ttgcaatgct cacacataga ccaacaaata aggctctttg
     6841 cataaatccg taactaagca tagtttaata cctcgcattt tattgtatga ttctcacata
     6901 ttaatatctt gtctgcaatt ttttttaatt gttcataatc atgtaatata agaagaattg
     6961 tatgtccatt attatgtaaa tccattatca tatccattac agatatttta tttacctcat
     7021 ctagtgcatt taaaggttca tcaaataata ttatatcggg atgattcata attgatttta
     7081 atatcattga tttttgtaat tgaccaccag atagagattt aagctgtttt tttgcataat
     7141 gttccatatt atactttctt agtagcatca ttacattttt cttattttta aacaactttg
     7201 acttttcttt atgttgatac attagtaaaa actccatcaa tgttaatggc gaaccttctg
     7261 ccatcactct atcattttga cctaaaaaac tcgtagttaa tgaatcaaaa gtaaaaatct
     7321 ctccactttg taacttaact tcctttgtaa taattttaaa tagtgttgat ttcccactac
     7381 cattttctcc ataaactcca accaactcat ttttatttat atttacattt acacttttaa
     7441 aaacattgca atctgtatac gaaaatgctc catcttttat ttccactata tttttcatac
     7501 ttattttgat aatgcatata ccaagttttg aagattagtt ttcataactg atatatagtc
     7561 ctccccctct tttatttttt tatcagataa ttcttctaat gtgtttaata cttttgtttc
     7621 tgaacctgtc tcttttgcta ttgtctcagc tacttttgga tttacaagtt cttcataaaa
     7681 tataaccttg attttattct ttttagcaga tttaataatt tctgccatac gtgcagggct
     7741 tggttctgaa tctggagaca atccttcaat tgcaatctgt tttaaattat agtctttgca
     7801 aagataacca aatgcttgat gagccacaac tatagaacgt cctttatatg gaagtagttc
     7861 ctttttgtat tgtgagtcta aagcatcaaa ttcacttgca tacttataat aattcttctc
     7921 ataatatgta gcattatctg ggtctttttt acaaaaagca ttttttattt tctctaactc
     7981 tttctttgca ttacgaatac taagccatgt atgagggtcc atactgtctg aacttttttc
     8041 tcctttcatc aaatcaattc catcacttgc tttgacaatc aaaatatttc tattatccaa
     8101 gctttgtata acatcattga cccaatgttc catattgtct ccattgtaga taaacatgtc
     8161 agcattgctc aatcctttta tatcattcgt tgttggctca aaatcatgag gctcaattcc
     8221 agcaggtgtc atgtttttaa catcaacctt agttccacca atttttttag caaaatcata
     8281 cattggataa aaactagtat atacactcaa ttttttgttt gagcttgtct ttttaacatc
     8341 tttgaaacta cacccagtca atagtaataa actacagcaa aataataata caaatctttt
     8401 cataatcgcc ctcctttttg caaatgcttt gcatttgcat ttttataagg attatctcat
     8461 ttttttctct atttgtcaac taattgtgtt gttaaattat atcttatgat atatgataat
     8521 aatagaatca taaaagaatg gtggttttat atatgaataa ttatttgaaa aaacacaact
     8581 taaagagtac agaaaaaaga gaggctgtaa ttaaagtatt agaaaatagc aatgtaccta
     8641 tgacaataga aagtattcac gaaaaagcaa atactatagt agaaatgaat ttaagtacta
     8701 tttacagaac aataacaact ttatgcgaaa agaatattac agttttagta gctatacatg
     8761 atggaaaatc ttattatcag cttaacaagc atagacatga gcatgtatta atttgtaaag
     8821 aatgtaaaga tattattatc attgacgaat gccccttaca tgagctagaa cataaacttg
     8881 aagatgaaac tggttttgat attatcgaac ataagttaga atttattggc atatgtccta
     8941 aatgtaaaag aacgaactct agaacataag ctcatctctc ttttttattt aacttgtttt
     9001 tcttattaaa tatataatta tagattatat aaagttaata aggaaagata attgcaaggc
     9061 ttaatgaaat tatttttaga aaaagtttct actaagtctt gaaatactta tatatattat
     9121 ttcaataaaa ttttatttct aagataataa ttaaatctct atttattaac ttaaatttaa
     9181 atttatttct tcaaaataaa cacttaaata agctataagg aataacccta tctgtaattt
     9241 tagctactag cgaatgataa aatatatcaa ttctgtagat acttttaatg tgtattgata
     9301 aagacttgtt tctgttaaac ataaaaagtg tgctccttcc tttatatgat gatatttgat
     9361 attttaatta tcaccacttt tggaagaata cacactttat tttaaatacc ttttgtactc
     9421 tattttatat aatttctaac aacttaataa aaatgcttta attgttttaa cttacttgga
     9481 tgtcttaact ttcttattgc tttagcttct atttgtctta ttcgttctct agtaactcca
     9541 aatacccttc caatctgttc taatgtctta gggtcatcat catcaattcc aaatctcatt
     9601 cttaaaactt gctgttcttg ctctccaaga cctgtaagca attcttctat tttttctttt
     9661 aaattatgtt gttcaacctc atgcatcaca acatccttaa aatcagcatc tgatggtata
     9721 aattcaacta aactactatc ctcatcttct tttaatggag tatccaaaga tacaacattt
     9781 ttatctcgtc ttaataattc caatacttta tcaacttcta atccacatgc atctgcaatc
     9841 tcatctatag ttggctctct taataataca ttcaaaagtt cttgtttctt tttctttaca
     9901 aaatttattc tctgatgaag atgtataggt atcctaatag tattttcgca atcgtcgata
     9961 tatcttgtta tactttgttt tatccaccaa gttgcataag tgctaaattt gtatcctttt
    10021 ttataatcgt acttttctac agcttttata agtcctatat ttcctgcttg aattaaatcc
    10081 aacatatcaa tactatctct tttatatctc tttgctatgc tcactaccaa cctataattt
    10141 gaatttataa atttttcttt tgctactgaa gatgagttga ttatttcctt agccagctct
    10201 acctcttctc ctgctgacaa cattttgtat tcctctattt cctttaaata cattttcatc
    10261 gcatttgata catttgatga acccacacaa atataatttt cataattatt ttctttttta
    10321 gtatccataa tcaaacccct tctattcata gaatttgcta aaacaaaaat aaataattta
    10381 aacctacata agctacaatt acataatatc ccattcttca aatgaattgt tttgtatatt
    10441 atgtatacaa atttataaaa actataccta taatattatt ctacttctaa cagcaatatc
    10501 ctccttatat atataattta ttttattata tgttgttata ttcttagtct ttagaatgat
    10561 atatgtctaa aaaaagactg tttactaaac agtctttttt tatatatatg ctcaatattt
    10621 aattttcatg atgatgatta catccacatc cacaattttc atcatggtga tgatgttctt
    10681 ctaaatcttt tacctgttgt tccatttcat gaaactcttt taccattata tcataagttg
    10741 gaagagctgc ctcagtatcg tattcttcac ctctccaatc agcgtacatc atagcatctc
    10801 cattagttaa aaccaatcta cctgacatag ttatattgtc cacttcttct aaaaattcta
    10861 tttgtttttg taaaacttct ccttctaact ccacatcttc atctaaacat tgtactatca
    10921 ttacaggtgc ataataatct tcttcatctt taacatttac aacaatatct aaaattcctt
    10981 ctgcaccttc ttcaaactct gccaactcaa catttgtatc aatcatatct tgaggtacta
    11041 ataagtcttc tattaaatat tttattactc tatcttttac taattctttt gacatttatt
    11101 tttctccttc cgattctata acattatcat aatcatttct atattatagt atactacatt
    11161 aattgttttt tcaatctctt acaaaaatta tttttttgtt atcttcctta tatttccttt
    11221 tttattacaa agttgtaatc gcattgattt aaaaaatagt ttatatttag tataatagaa
    11281 atgtaacaca tatttttatt caatatatta ataaaaaata gattgaaaat gtaatgtatg
    11341 ttaaatctag gaggaatatt atgaagttta aaaaacaaat tagttctatt gttgtgtcta
    11401 ctatgcttgt acttggaagt atgaatcttt cttatgcaga tggtacaaat gtagttacac
    11461 ttggagctaa cttatcagag tctcagaagc agcaaatgct taattacttt ggagtaaaaa
    11521 aagaccaagt cttagtttta gatgtaacaa atgcagaaga aagacaatat ttacaaggtg
    11581 ttgcaacaga gggtcaactt ggaaaagtta ctatatcttg ttcatatgtt gaaccaacta
    11641 aaaaaggaaa tggaattaat gttaaaacag caaacttaac ttgggtaact agctcaatga
    11701 ttgcttcaac acttacaaca gctggtcttg aagatgcaaa tgttattgct gctgctccat
    11761 atcctgtaag tggtactgga gcattgacag gaatcatgaa agcatttgag gatgcttcag
    11821 gaaagaagct tgatgagacg aagaaagaaa ttgcttctga agaattggtg gttacaggtg
    11881 atttaggaga cgatataggg caagaaaaag caacaggtgt aatgaatgat ataaaaacag
    11941 aaattgtaaa gaatggtaca aaagatacca atcaaatagc aaatacaata aataatgttg
    12001 taaataatta caatattact attacaccag aacaaaaaga acaaattaaa ggtgtaatga
    12061 aaaaaatagc tgaccaagat tacgattata atagcatgaa aaatacacta gataacgtat
    12121 cagataatgt aaatgaacaa ttaaagaaat taggtgaaag tgttaagagt tctggtatac
    12181 tagatactat tggtggatgg tttagtggaa taggagactg gttctctggt ttattctcag
    12241 gtggagataa aaaagattta ggaatcttaa ataacactaa tgatgagtct cttggctctg
    12301 atgctgtaat aaattctaca gaaggtgttc aaataaaccc acaaaataat actaattcta
    12361 acagtgacag tgatacaaat aactcaaaga ataatacttc aactaataat gaaaataaag
    12421 ataattctca aacaaatgaa aacgacacaa caaataattc agaaaatcaa tcaaataata
    12481 atgactcagt tggtttcttt gaaaaaataa aaaattggct ttcaggccta ttttaaaaat
    12541 taaacaaata aaaagaattg cctaaaatgg aaattttcta taaaaggcaa ttctctttat
    12601 ttacaataaa tttttattac tcactcattt ttttagattt ttctatacat gcacatatcg
    12661 cttttatcac agaagctcta aaaccttctt cttcaagtac tttaactgcc tctatagtag
    12721 ttccaccagg tgaacatacc atatctttta actcaccagg atgtttccct gtctcaagta
    12781 ccatttttgc agagcccata acagcctgtg atgcaaactt atatgcttgt ggtcttggca
    12841 ttcctgcaat tactgctgca tctgctatag cttctataaa taaaaacaca tatgctggtg
    12901 cagaaccact tgcaccaatt acagcttcta taagatactc tggaactacc tcagtatttc
    12961 caaaagaatt aaatacttct gtaactgctt ctaagtcttc atcgtttata tttttattta
    13021 tagaaagaga agacatggct tcatttacaa gagctggagt atttggcata gttctaacta
    13081 tttttttatc atctcctaca atagactcta tatcctttat agattttcct gctgctattg
    13141 taactataat tttattgtca tctataaaat cttttatctc ttccaaaata tcatcatata
    13201 tatctggttt aacagccaca attactatat cagagcttct agttacttcc tttgaatctt
    13261 ttgtagtgtt tattccaaaa tttgcatata ttctatcaag agtttcttga ctatatgctg
    13321 atgctataac catattaggt tcaactaatt ttgaatttac tattccccct atcatagcgc
    13381 ttgccatatt tcctgcacct atgaatccaa tttttttcac tttagtacct ccttattagc
    13441 taatagtagt tgctaaaatt taagcttctt ttacttcatt agatttttca aatggatttg
    13501 gtacacttac taccaaaatt ttagctacct tattagtata gttaacccaa ttatgattgt
    13561 tcttttcact attatagtgt attgtgtctc ctggatacat ttctatttgc tcatctccca
    13621 aaaacactgt aagagtgccc tctaatacat acacaaactc ctcaccttca tgtacataac
    13681 aacaaatttc ctcactactc ttacttggaa gaatttcaat taatctcggt aacattgttt
    13741 tttcctttaa actagatgat aaatgataat gtataaatgt tgagttttct acatcaaata
    13801 cttctttttc ataacttctt aatacagctc ttttatgctc ttttggcttc ataaaaaagt
    13861 aagttaattc tacatctagt acacttgcta tttttcctaa ggaatctgtt gccacacttg
    13921 ttaatcctct ttcaagttgt gataaaaaac caattgacaa gtttgttttt tcactcatat
    13981 ccttcagtgt catttgcttt tgagttctga gttgctttat ttttgctcct atgtccttat
    14041 tcaaaaccat cacccctaaa taattttaat tttttcataa tataaataca aaaatataaa
    14101 tataggtatt aaacctacct ttatttcatt tttaaaaaat tttataagta ttactataat
    14161 ttattaatct ttttttgtct acatttttac gattatttac gatttatttt tcacacatgt
    14221 atatttaatt tcgccaacta aatatacctt ttttataagc atctactctt tatatataaa
    14281 acttaagtta ttcatacaat tttctttagc tttagttatt aaaatttcaa attatattat
    14341 tatttctaca cattattaca tcctttagta caattataac ataattttac aatttaatga
    14401 tatctattca atgtcttttt ttactctaca acatatttta ttttagagtt tagcactata
    14461 tatatttttt atatacagtc ttgtcaattt ttagattgtc aagaattatt gaagtatttt
    14521 atgaccattt aaattttatt ttctaattcc ttacttaaca aaaattgctt a
//
LOCUS       DAMJVM010000088        14254 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.092, whole genome shotgun sequence.
ACCESSION   DAMJVM010000088 DAMJVM010000000
VERSION     DAMJVM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14254
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.092"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            469..1362
                     /locus_tag="PC523_002765"
     CDS             469..1362
                     /locus_tag="PC523_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HDF2476272.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELKNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            1362..2756
                     /gene="gltA"
                     /locus_tag="PC523_002766"
     CDS             1362..2756
                     /gene="gltA"
                     /locus_tag="PC523_002766"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HDF2476273.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            3121..5340
                     /locus_tag="PC523_002767"
     CDS             3121..5340
                     /locus_tag="PC523_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2476274.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIETSKRELENYLEINISLLKALSKDDRFSDSETSLIEKGKLLRPYQKEYNLFMIGI
                     TDTKGNTSSTYREKVGSIKDRPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIIKN
                     NEMIGTIFASFYFQDVNNLISRSNLDDSIKFLMIDKNYTIISHPNKKYVNDKSKILDL
                     EGNIIGTTKSEILKNINEKCQGKFLYWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFQ
                     SLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACSTEDELLNRLSKINKEICNLSTTNSSKYKIVMSSGIYKINKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEETRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LNDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLREIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            5493..6239
                     /locus_tag="PC523_002768"
     CDS             5493..6239
                     /locus_tag="PC523_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2476275.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIDKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVILDGYDISKLEGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDINIQTRNISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTKINIEQNVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            6239..8119
                     /locus_tag="PC523_002769"
     CDS             6239..8119
                     /locus_tag="PC523_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2476276.1"
                     /translation="MDFKKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     MKSGIQSGFTAFMALMTSELIILGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTELYFPVKAILLTVVS
                     FMILFLITGPMSLKLLNKKSILELLLGTKKPKKEIKSSKLFGIAGIVCLILGYAMILF
                     GNKLKINGGMYTTTLLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLMYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGESSKNNFPMVYSYFSEHNNEKEYEE
                     LQKIRDTIKRYDYKELSFPILQYNREVLLNVKDYNKLSKELGLDKISSKLRKYEGIII
                     PSSNSQEHINSLKELRNYSIKNLNIKIKKSTNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKMNFHGFDYKNWQNSAKITENLKVKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEMEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFNLENLGILLLISSIYSLYFYLLKKFYTRKVWF
                     FD"
     gene            8238..9629
                     /locus_tag="PC523_002770"
     CDS             8238..9629
                     /locus_tag="PC523_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2476277.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAVLTIGYYIS
                     GKSNLRFSKSNLKLDTKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLAVATIILCMGMV
                     VIMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            9736..10776
                     /locus_tag="PC523_002771"
     CDS             9736..10776
                     /locus_tag="PC523_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2476278.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDESL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            11268..11921
                     /locus_tag="PC523_002772"
     CDS             11268..11921
                     /locus_tag="PC523_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2476279.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFIEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIDGIIDGMKF"
     gene            11997..12206
                     /locus_tag="PC523_002773"
     CDS             11997..12206
                     /locus_tag="PC523_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476280.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNVVMIDDGTGTLKQAIF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(12346..12912)
                     /locus_tag="PC523_002774"
     CDS             complement(12346..12912)
                     /locus_tag="PC523_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDF2476281.1"
                     /translation="MMNNELSTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNMPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDIAKK"
     gene            13072..13725
                     /locus_tag="PC523_002775"
     CDS             13072..13725
                     /locus_tag="PC523_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDF2476282.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVSLHETSLL
                     MIISVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(13883..14059)
                     /locus_tag="PC523_002776"
     CDS             complement(13883..14059)
                     /locus_tag="PC523_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476283.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT         5607 a         1422 c         2356 g         4869 t
ORIGIN      
        1 attttagata tataataaaa taagagtgtg ttttatataa tatgaaagct ggtattgcaa
       61 tagattaaag tcttatacac gtttagtttt taaagaaatt agtgttatta tgaaaaccta
      121 agttaactta ttaaataaat aattgaataa aaaaaattta ttatgtattt ataaaatttt
      181 tgttacaaat ttggcataat atagtatact ttaagtgtgt attaggttaa atctataaca
      241 aattagacgt atgatataga ctgagtgttt attggcattt acaagaaatt tgttaatatc
      301 aactatttgt agtttgggca gttataatta ctataggggg gatgtccaat acaaaacaat
      361 caaccaagaa ctaatattat attataaaaa gttatagttt tgtgtgttta tttttttaat
      421 taaatttaaa gtgggaaaaa tatatttgtt agaatggaga atagcgagat gagtaataaa
      481 atagttagta agagacaatt aacagatagt atttatttaa tggaaataga ggcacctaga
      541 gttgcaaaat cttctcaacc tggtcaattt ataataatta aaaatgacga aaaaggagag
      601 aggattcctc ttacaatagc tgactatgat agagaaaaag gaacagtaac tattgtattc
      661 caaacagttg gtgcttcaac taaaaagtta gcaatgtttg aagaaaacga ttttgtaatg
      721 gactttgtag gaccactagg acaagcaagt gaatttatac atgaagatat agaagaatta
      781 aaaaataaaa agatattatt tgtagctggt ggagttggtt ctgctccagt ttatccacaa
      841 gttaaatggt ttaaagagca tggtttagat gtagatgtaa taattggtgc aagaactaaa
      901 gaattaatta tattagaaga tgatatgaaa aaagtagcta aaaatgtata tgtatcaaca
      961 gatgatggta catatggatt taatggaaga gttacagacc tattaaaaga tttagttgat
     1021 aatcaaggta aaaaatacga ccaagcaata gtaattggac caatgataat gatgaaattt
     1081 atgtgccaat tgactaaaga actaaatata cctactatag taagtttaaa tacaataatg
     1141 atagatggaa caggtatgtg tggtggttgt agagttagcg ttggaaatga aactaagttt
     1201 gcatgtgtag atggtcctga atttgatggt catttagttg attttgatca agcaatgaga
     1261 agacaatcta tgtacaagac tcaagaggga agagctatgc taaaacttga agaaggcgat
     1321 agtcatcatc atagtaattg tggatgtgga ggtaacaaat aatggatgct aaaaaagtaa
     1381 aagtaccagt tagagagcaa gaacctgctg ttagagcaac aaattttgat gaagtatgtt
     1441 tagggtataa taaagaagaa gctatggcag aagctaatag atgtttagct tgtaagaaac
     1501 ctaaatgtgt aggtggatgt ccagtaggaa tcgacatacc agggtttata acaaaaatta
     1561 aagaagacga tatagaaggt gctgctaaag taatagctaa gagtagctca ttaccagccg
     1621 tttgtggtag agtgtgtcca caagaaagcc aatgtgaagg tgtatgtata cttggaataa
     1681 aatcagatgc tgtatcaata ggtaagttag aaagatttgt agcagattgg tcaaaagaaa
     1741 atgacataaa tttatctgat acagagccta agaaaaatca aaaggtagca gtaataggaa
     1801 gtggtccagc aggtctagct tgtgctggtg acttagctaa aaaaggctat gatgtaacaa
     1861 tctttgaagc aatgcatgag ccaggtggag ttttaactta tggaatacct gaatttagac
     1921 ttccaaaaca agctgttgtt caacctgaaa tagataatat aagaaaatta ggtgtaaaaa
     1981 tagagactaa tgtaattgtt ggtaaaacaa ttacagtaga tgaacttata gaagatgaag
     2041 gatttgaagc tatattcata ggttcaggag ctggtcttcc aatgtttatg aatataccag
     2101 gagaaaatgc aaatggagta ttttctgcaa atgagttttt aactagagta aacttaatga
     2161 aggcttatag agatgactat gatacaccta ttagttctgg taaaaaagtt gctgtagtag
     2221 gtggtggaaa tgttgctatg gacgctgcta gaactgcact aagattgggt tcagaatcat
     2281 atatagttta tagaagaagt gaaaaagagc tcccagcaag agctgaagaa gttcatcatg
     2341 caaaagaaga aggtattata tttaatacac taacaaatcc taaggaaatt ttagtggatg
     2401 aaaatggata tgttaaaggt atggtttgta taagaatgga attaggagag ccagatgatt
     2461 ctggaagaag aagacctata gaaatagaag gttctgaatt tgtacttgat gtagatacag
     2521 ttataatgtc acttggaaca agtccaaatc cactaatatc ttctacaaca aagagtcttg
     2581 atataaataa gaagagatgt ttaataacag atgaaaacgg tcaaacttct aaagaaggtg
     2641 tgtttgcagg tggagatgct gttactggag cagcaacagt aatatctgca atgggtgctg
     2701 gtaaaacagc agcagcatca atagatgaat atttaaaagc aaaagttaat gcttaatttg
     2761 taaattaaag agagggtgtc tcaaaatgaa ctttgtagtc catgaggcac tctttttgta
     2821 caaaatcaaa tatattttaa tcatttcaac gatgaaaatt tcattcctgt ataaagtcta
     2881 acctttaggt cttagcacaa tctctatttt gatgtatcca ctttttcaga ttgtttaata
     2941 atttataagt tattattgta atatttaata ctaagtaaca taactctgta taactatgat
     3001 aaaatagtta tacaataaag gtctataagg agtgaataat gtgtggctaa taaaatcaga
     3061 tttgtcaaat ctagcattag ataatagaaa aataaattgt ataattaatg gtattttaat
     3121 atgaaaaaaa gagaaaaagt atttgatgga ttagttttta agcatatatt aattttacta
     3181 atattattag ttttgattga tttaacaagc accatcatat atgcaagtaa ttctatggaa
     3241 attacttcta gaaatatgat agagacatca aaaagagaac ttgaaaacta tttagaaata
     3301 aacatatctt tattaaaagc tcttagtaaa gatgatagat tttctgatag tgaaacatct
     3361 ctaattgaaa agggaaaatt attaagacca tatcaaaaag aatataatct ttttatgatt
     3421 ggtataacag atacaaaagg aaatacttca agtacatata gggaaaaggt tggctcaatt
     3481 aaggataggc ctagttttga aaaagcaatt aaaacaaagc aagtagtagt ctcagatatt
     3541 gaagttagca atgtgacagg tgataaggta tttattatat atgtaccaat tataaaaaat
     3601 aatgagatga ttggaactat ttttgcttct ttttattttc aagacgtaaa taatttaata
     3661 agtagaagca atcttgatga ttctattaaa tttctaatga tagataaaaa ttatactatt
     3721 atttctcatc caaacaaaaa atatgtaaat gataaaagta agattcttga tttagaagga
     3781 aatattatag ggactactaa aagtgaaatt ttaaaaaata taaatgagaa gtgtcaaggt
     3841 aaatttttat attgggataa ttggagatta tataatgtaa aatatacgaa cataaagtgg
     3901 accaattgga ctttagtatc aaaatgtaat atatttaaga actttcaaag tctaatagtt
     3961 aattttatga taaagttgta tttttatata gtaatattta tgatattgtg gaaattaagt
     4021 aacgctaagt taatagaaca acttaaaaaa ttggcttatt atgattcttt aagtggaata
     4081 aaaaataaag aaaagtttag aaaagattct atgtatattt taaagaatta ttatcaggat
     4141 aatttttact tagtacaatt agatgtcaat aaatttaaat acataaatga aatgtttggc
     4201 tatgctgaag gaaataagat attaatacat atctcacagg ttttaaataa taatacaaat
     4261 aaatatgaaa tttgtgctag gatggataat gaccatttta tattgttgat tgcttgtagc
     4321 actgaagatg aacttttaaa tagattatct aaaataaata aagaaatttg taatttaagt
     4381 actactaact catctaagta taaaattgtt atgtcaagtg gaatatataa gattaataaa
     4441 aaggatgata ttaaaaagat tgacctgtta attgatagag caaatattgc agccaaaagc
     4501 aaaaaggaaa aatatgaaca ctcatattca ttttttaatg aagaaacaag aaatcgattg
     4561 tataaggaaa aacgattgga agataatatg aataaagctt tagaaaaagg tgaatttata
     4621 gtgtattacc aacctaagta tagtttaaat gatgtaaatg aaattgaagg agcagaagct
     4681 ttgataagat ggaatagtcc agaatttggg tttatttcac ctatagattt tgtaccttta
     4741 tttgaaaaga atggttttat tgtaaatatt gatatgttcg tgtttgaaga agtatgcaaa
     4801 acattaaata agtggataaa taaaggttat actcctgtac caatttctgt aaatatgtct
     4861 agagttcatt tatataggga caattttata gaaaatataa ctgatttgat aagtaaatac
     4921 aatatatctc ctgagtttat tgaacttgaa ctaacagaaa gcgttgtgtt tgataattta
     4981 aatatattga ttgatataat gaaaaagatt aaagaaatag gatttcttat atcaatggat
     5041 gattttggtt caggatattc atctttaaat ctgttaaaag acctttcttt tgacatttta
     5101 aaattggata gaggattttt aatcgaaaca acagatacaa aacgtggaaa aattattatt
     5161 tctaagattg tggagatggc aaaagcgatt gatattaaag taatctgtga aggtgtagaa
     5221 acttatgaac aagtagagtt cttaagagag ataggttgtg ataaggttca aggatactta
     5281 tttgctaagc ctatggtatt agatgaattt gaaaaacact taaactttaa atttgattaa
     5341 tcataatcat taataattat tatactatta gatactctaa caacttactt acaaatatgt
     5401 aagttaattg ttagggtatt tttatggtta aaatagtata ttcacgatat tatggtattg
     5461 tagtaaaaat aaaaagttat ggagaatata aaatgaacat attgagtatt agaaatatat
     5521 caaaaacata ttgtggaaat attccattta aagcattaga taaggttagt ttaaatatag
     5581 ataaaggaga atttgtgtct gttatgggac catctggaag tggcaaatct actcttttaa
     5641 atataatatc tacagttgat agacagagtg aaggtgaggt tatattagat ggatatgata
     5701 taagtaaatt agaaggagaa aaacttgctg aatttagaag gaaacaactt ggttttgtat
     5761 ttcaggattt caatttgatt gatactttaa ctgttggcga aaatattatg ttgccactta
     5821 cactagaagg ggcaagtatt aaagatatta atatacaaac tagaaatatc tctaaatttt
     5881 taggaataga taaaatttta gatagaaaaa cctatgaaat ttcaggaggg caagctcaaa
     5941 ggtgtgctat tgctagagcc attataaata aacctgccat acttcttgca gatgaaccaa
     6001 ctggaaatct tgactcaaaa tctactgatg atgttctgaa attatttact aagataaaca
     6061 tagaacaaaa tgtaacaaca cttatggtaa ctcatgaagc atatagtgca agttattcag
     6121 atagagtgat ttttataaaa gatggttgta tatatacaga aataaaaaaa tctgaaagca
     6181 gtaattcttt ctattctgat atactagctg tattgtctca aataggaggt gtaagatagt
     6241 ggattttaaa aaatttgctt ataataatat agttagaaat tttagagcat atttagcata
     6301 ttttttaagt tcggtttttt ctataatgat attttttgtc tttgcagtat ctatgtttca
     6361 tccaattata atgaaatctg gaatccaaag tggctttact gcatttatgg ctcttatgac
     6421 ttcagaatta ataatcttag gttttagtct actttttata ttgtattctt taggaaactt
     6481 tttaaaatat agattgaaag attttggagt attgatgata attggaatga gtaacagaca
     6541 actgaaaaaa ttgatattaa ttgaaaattt gataataggg tttttagcag tgatgttagg
     6601 aatattactt ggtttatcaa tttccaaatt gtttttattg tatttaagca aactgtttta
     6661 tatgaattta actgaacttt attttccggt aaaagcaata ctattgactg ttgtatcatt
     6721 tatgattttg tttttaataa caggaccaat gagcttaaaa ttattaaata aaaagagtat
     6781 tttagagctt ttactaggta caaaaaagcc taaaaaagag ataaaatctt caaagttatt
     6841 tggaatagct ggtatagtat gtcttatatt aggatatgct atgatactat ttggcaataa
     6901 gttaaaaata aatggaggta tgtatacaac aacactgtta attactttgg ggatattttt
     6961 atttttttct caatttacga ttttgatatt aaagtattta aaaaataagg aaaaatttta
     7021 taagaacaaa gtaaatatgt tacttataag taatttgatg tataaattaa aggataatac
     7081 acgattgtta tttttaataa caattctttt atcagccact ttagtagcat ttacaacaac
     7141 ttcaacactt gtaagttctc aaggagaatc ttctaaaaat aattttccaa tggtttactc
     7201 atatttttca gagcataata atgaaaaaga atatgaagaa ctacaaaaaa tcagagatac
     7261 aatcaaaaga tatgattata aggagttaag ttttcctata cttcaatata atagggaagt
     7321 cttactaaat gtaaaggatt ataacaaact atcaaaggaa ttgggattag ataaaattag
     7381 ttcaaagtta agaaagtatg aaggtattat aattcctagt agcaattcac aagaacatat
     7441 aaactcttta aaagaattaa gaaattatag tataaaaaat ttaaatataa aaataaaaaa
     7501 atcaacaaat aaaataattt ttcctacagg attgttcagt aaagtagtag ttataagtga
     7561 cgatttatac aatgaagtaa agggtgaatt ttcaaaaatg aattttcatg gattcgatta
     7621 taaaaactgg caaaacagtg caaagataac tgaaaattta aaagtaaaac attttaattt
     7681 ggatagagaa tataacaggt catatttttt aacattacca gatatgtatt tagtagagtt
     7741 acaacaaagt aaaattcttc aatttatggg tgtttttata ggagtcatat tatttatagc
     7801 agtttttagt tttttatatt tcagattata tacagatgag atggaagata atgtaaaata
     7861 taaaaaacta tcaaaaatag gtctatcttt taaaaatatg aataagattg ttagtataga
     7921 gataggaaca ttatttttta taccgtattt tatagcaatt ataaatagca taatttcttt
     7981 attgttttta aatcaagtat taaataatag ttttaactta gaaaatctgg gtatattact
     8041 actaatatca tctatatatt cactttattt ttatctatta aaaaaatttt atactagaaa
     8101 agtttggttt tttgattaaa gtggtatata atagatatat aaattaatat ttaaaaagta
     8161 gttaatatta ggggagggca tttgcacctc ccttgtaata tgtggaaaat tgaatattag
     8221 aagaaggagg agacttgatg gaaaatcaac aattattagg aacagaacga attagtaaat
     8281 tactactaaa atattctata ccagctataa taggtatgtt ggtaaatagt ttatataatg
     8341 tagtagatag aatttttatt ggaaatatac caggagttgg accacttgca ataacaggtc
     8401 ttggtgtgac aatgccaata atgacaataa tacttgcatt tggaatgttg ataggtatag
     8461 gtactactac taccatatca ataaaacttg ggcaaggtaa agtagaagag gcaagaaaac
     8521 ttataggaaa tgcaatgacg ttatctgtaa taacaggaat tatcataatg atacttggaa
     8581 tattatttgc aaataaaata cttacattgt ttggagcaag tgaaaatacc ttaatatatg
     8641 caaaatcata tataaatatt atattattag gtacagtagt aaatcttctt tccttttctt
     8701 taaaccactc aataagagct gatggtagtc ctaaaatttc agcaggaata atgatagtag
     8761 gatgtttaac taatatagtt ttagactgga tattaatatt tggattcaat ttgggaatac
     8821 aaggcgctgc aattgctaca gtaacttctc aagctcttac agctgtttta actatagggt
     8881 attatataag tggaaaatca aacttacgat tcagtaaatc taatctaaaa ttagatacaa
     8941 agctaataaa agcagtgttt gcaataggaa tgtcgccatt tgcgatgcaa cttgcagcaa
     9001 gtttagttca agtaatatca aatattgcac taaaaactca tggtggagat ttagctatag
     9061 gggctatggc tactatttct tctattgcaa tggtattttt gatgcctatc tttggaataa
     9121 atcaaggagc gcaacctatt ataggtttca attatggagc tgaaaaatat gatagggtta
     9181 aaaaagcata tttaggttca ttagcagtag ctactataat tttatgtatg ggtatggtag
     9241 taattatgct tttcccagaa gctattatag gtatatttaa taaagaccct gaacttatga
     9301 atatatctgt taatggatta agaatatact tacttatgtt gccaattgta ggcttatcag
     9361 ttacaggaac aaattttata caatctatag gaaaagcaaa aatggctatg ttattgagcc
     9421 ttttaagaca agtaatttta ttgataccag cagtattaat tcttcctaca ttcttaggtt
     9481 tacagggtgt atggacagct caaccagtgt ctgattttat agctacagta attacaggta
     9541 tagttgtatt tagagaatta aaaagatata ctcctaaaac tgaaaaatta aatgaaaatg
     9601 aaagattaaa tgagataact actgagtaaa aatataaatt cagataattg agcaagaaat
     9661 tgcaaaaaat acaattatat gataaaataa aaccgctgtc aaggataaga gaaattctaa
     9721 ttatggagtg attttatgga atttacaaag aaaagcaaat taaaaaatat gtatgtaaac
     9781 atgaatgtag ctagaatgtt gtctaagata aatgagtata agggtagaca actgctttac
     9841 aaaaaacaac caaaagaaat tttagaaaac ttagaaaaaa aatctttagt agattgttct
     9901 gaatctacat acattggaaa tcaaagagat agtagtaatt ttaatctaga aaaattgata
     9961 agtaatgaag ttacacctag aagtagagag gaattgagta ttgttgaata cagagatgta
    10021 gtaaaaacta ttaacagtgc ttatgagagt attccaatca gttcacaaac aattcttgag
    10081 cttcatggat acttatataa attttcatct actagaggag gaagttataa atcagataat
    10141 gattttattg aacacaattt aaaacctagc gaatttataa gtattgattc aagtgtaaat
    10201 aaagttgaaa aagctgtaga agaaatatgt gaagcttata atacattgat agaggaagat
    10261 gaaattgata tattaatatt aatttctgca tttgtgctag atttcatatt gatacatcca
    10321 tttaaagaag gtaatataaa aatggcgaga gtacttatcc tattgttact aaataaaaat
    10381 ggatatgaag ttggaagata tataagttta ggaaagatat ttgatgatag ttcatatgaa
    10441 tattatagta atttaaatta tttgaaagcg tctataggta gtgaaaaagc agatatgaat
    10501 gcatggattg agtatttttt agagactata ttgactgcat atgagaagct agatgagagt
    10561 ttaaatatat ctgataagaa aagacagaca aaaactagta gaatagagaa aataataaat
    10621 tctacccttg gatattttac aaaagaagat ataagagatt tatgtcctga tattccagaa
    10681 cctactataa atagagtttt taacaatcta agaaaacaag ataagataga agttgttgca
    10741 aggggaagaa gtgcaaaatg gaagaaaaaa tattaaaaaa tatttaaata actaaaatgg
    10801 tatagtgttt tgcactatac catttttagt atcactagca tgggcaataa tttaatagct
    10861 taagcaatat caattttatt tattgattat ttttgaactg aaaaaaggga ttatatggat
    10921 acctcaatgg taattaattt tggaggagaa gatataacat tacagaatat cttaggtgta
    10981 tatgtagaaa aaataaaata taataaacgt gtgaggttta gtaattaaaa aattatgtag
    11041 cttcgcactt ttttataaaa taaagatact tagcagaata atattttagt tgatatattt
    11101 ataaagaggt gcttgtttgg gtaaaagtat aaataataaa aagtagaagt catgtatata
    11161 aaaatataag ctatattcta taataataat tattttataa ttcaacaaaa gaatagaatg
    11221 ggtatatttg tgaaataata atataaaata atcttaggag tgagcttatg aagaaaaaat
    11281 tattatatat aaatgtaaat tcaaagcctg aagatttatc ttcaagtaaa actgtagcaa
    11341 gaaaatttat aaataaattt atagaaaaaa acaaagattt tgaggttgaa gagatagacc
    11401 tttataaaga acatatacca agacttgagt atcagtactt tgaaaagaga aatagcattg
    11461 ttagcgaaga aaatgctaaa aatctagacg acaaagatag aaaagaactt acaaagatta
    11521 gaaatctttg tgaccaattt gtgagtgcaa gtgtatatgt aatagcagca cctatgtgga
    11581 gtttgtcttt tccagcacca cttaaagaat atatagattg tataattcaa gatggaaaaa
    11641 ctatatcttt tgaaggaaat gataaaccac aaggaatttt aaatgatatt gatagaagta
    11701 tggtatatat acaatcctct ggtggacaca taccatgggt attgaagcca gttatgaata
    11761 aagggttaaa ttatgtggaa agtattatga aatttatagg aattaaaaag tttgatgaat
    11821 tgttagtaga tggaactgga actagtgaag aagaaagaca agctgcaata gaaaaagcat
    11881 ctgaaaaaat agatggtata atagatggta tgaagtttta gatataatat tttattaaaa
    11941 atataaagga aaaacctctt ttaatgtaga aacttatatt gaaggaggcg agaatcatgc
    12001 gagaagaaaa aagtaatgaa aagtatgatt gttattggtg taatcaagag aataatttct
    12061 gtgtagaaat aaaagataat gtagttatga tagatgatgg cactggtaca ttaaaacaag
    12121 ccattttcat agggtataaa caaatccaaa ttaatttaaa ttgttcacat tgtcaaaact
    12181 taaatagaat aaaattaaat ttgtagaatt ttaaaatgga acttattgaa ggaattaaaa
    12241 atagaaagtg ttaatattta gactgaaaat tatattttat acaataagag tttatttaaa
    12301 taacattgta tcttaatatt tattttcagt cttactttat taaaattatt ttttagctat
    12361 atctggaaaa tatctatcta ctaatttttc tgaaatgttt tttaattggt cagccattga
    12421 agttaaactt aataaggcta tcaataaatc atctttattt tctggattta tactgatatt
    12481 ttcaaaatta gactctataa agtctggcat attctttctt tgattttctt ttatcaacat
    12541 gaatttttcg taacatgaaa ctaaatcttt ctcactgaaa ttatcctttt catacacacc
    12601 atgtaagatt cttttaattt cttgtattgt aagtgtattt ttcatagaat aaataattat
    12661 catctgtaaa atttgttctt tagtatactt ttttccctta actggtttga ttaatccctc
    12721 tttgctgtaa ttatttatca tggttttagt aagaatttta tcactttcaa atcttttatt
    12781 agcagaaagc ttattgtcaa ttagagaaat aatttgatcc atatacaagt caatagaggg
    12841 aatatcgttt gaagttatat ctgcattatt tagtgtttct agtattatag tactaagttc
    12901 attattcatc atatgttacc tccataatat atataataca ctaaattaga aaataaaaca
    12961 atattaagac ttgtaaaaaa aacataaagt ggtataatta aatatataat atagtaatga
    13021 aaactatatt aatgagtgtg atttttaaat acataattgt aggaggatga aatgtatcaa
    13081 ttttttttaa agggtagaga cccaataagc agtttaactc attttatagg tgcttgttta
    13141 tctcttttag caactataat tttagttttt caatctgttt cattacatga aacttctctc
    13201 ttgatgataa tatctgtatc agtatttgga ctttcactaa ttgctttata cagtgcaagt
    13261 tcgtattatc attttttaaa gggtacacca gaacaagaat tgttttttag aaaagtagac
    13321 catgcaatga tttatgtatt gattgctggt tcttatacac caatatgtct aaattttatg
    13381 gaaaaaaaag aaggcataat atttgttaca gcaatatgga tagttgcatt cattggcata
    13441 ataataaaaa tattttggat ggatgcacct aggtggttat ctacgagtat atatttactt
    13501 atgggttggg caattgtttt tgatataaat gcgttcaatt caattcctaa ggattgcctt
    13561 agactattaa ttatggaagg tgtatcttat tctataggtg caattattta tataattaaa
    13621 aagcctaata taagtcctga atttggattt catgaaatat ttcatatttt tattatgata
    13681 ggttctttat ttcacttttt agcagtttta ttgtatgttt tatagtttta aaagattcga
    13741 ttaattgata ctaaaattct acaatgaata aaatggaact gccttatata gattatatct
    13801 tcgacaataa gacagttcct tattataaat attaaattct acttataata aaattttgat
    13861 aaaaatagtc cattcaaaat gcctattcca taattccttt aatgtacttt tcatttacga
    13921 catatagttt gaatttatgt actataatta tatcctctaa attctctgaa acactaacaa
    13981 ttctgacaca tttgtttcta atttctacag gcaaatctaa atcatttgtt aaattcacat
    14041 agtcatcata tttatacata aaatcatccc tttacattaa gatatactta ttatactata
    14101 ttttaataag tttttttaaa ttattacaat ttttcccgaa atttcatttt ttatacataa
    14161 aatttccaaa ttaggtgaag attaaatttt ttattgtata atcaaaagtt acatataaaa
    14221 tattttattc gattttttaa ataactattg ccaa
//
LOCUS       DAMJVM010000089        13986 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.093, whole genome shotgun sequence.
ACCESSION   DAMJVM010000089 DAMJVM010000000
VERSION     DAMJVM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13986
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.093"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            350..1543
                     /gene="nifV"
                     /locus_tag="PC523_002777"
     CDS             350..1543
                     /gene="nifV"
                     /locus_tag="PC523_002777"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HDF2476284.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1568..3493
                     /locus_tag="PC523_002778"
     CDS             1568..3493
                     /locus_tag="PC523_002778"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HDF2476285.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIDGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKENNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLSNGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3515..4510
                     /locus_tag="PC523_002779"
     CDS             3515..4510
                     /locus_tag="PC523_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HDF2476286.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHDVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIL"
     gene            4839..5312
                     /locus_tag="PC523_002780"
     CDS             4839..5312
                     /locus_tag="PC523_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2476287.1"
                     /translation="MLGSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNILKVDKATTTKAIQKLIKAGYVDKKQDEFDKRGYNLT
                     PTNKSLEVYELIIEEENRSIEICFKNFTDEEKQVVTKLLEKMSKNAENEWFKVKR"
     gene            5346..5840
                     /locus_tag="PC523_002781"
     CDS             5346..5840
                     /locus_tag="PC523_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2476288.1"
                     /translation="MIRKANMNDLESIMKIIKSTVKEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKKGI
                     ASKLVDFAEKLARENNVLYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5919..6458
                     /locus_tag="PC523_002782"
     CDS             5919..6458
                     /locus_tag="PC523_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2476289.1"
                     /translation="MKGMIYVIEFLKKRRSIRKYKDIEVEKEKLDKILKAALLAPSSK
                     GLRTWEFIVVDDKEKLIKLSQCRTKGGGFFLKNAPLAIAIIADKEKNDVWIEDASIAA
                     SYIQLQAHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPDKYSVECIISIGYPDEE
                     KKAYSDSDLDYEKVHFNNF"
     gene            6647..6958
                     /locus_tag="PC523_002783"
     CDS             6647..6958
                     /locus_tag="PC523_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2476290.1"
                     /translation="MGIFKTKIDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKNLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            6986..7672
                     /locus_tag="PC523_002784"
     CDS             6986..7672
                     /locus_tag="PC523_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2476291.1"
                     /translation="MKYSYKKCIIDSILLMLIVQLLRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNSILIEKCKLILFVVVLSLA
                     IISTYCTKGYVFFNVTMMTLSVFIVPIFEELFFREYIWNYLSNFIKSKIKIICITSIL
                     SGIYNIGYIDIIRNYIALYNNSSYTFEVIISKIIIGTVFGIVLGLVRYRFRDVRLCIL
                     LRSLFAIFIR"
     gene            7904..8788
                     /locus_tag="PC523_002785"
     CDS             7904..8788
                     /locus_tag="PC523_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF2476292.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YDYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEQPLKLDISSV
                     VNGEVRQNSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DVVECKVEGIGVLKNIVD"
     gene            8887..10062
                     /locus_tag="PC523_002786"
     CDS             8887..10062
                     /locus_tag="PC523_002786"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HDF2476293.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGIPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDNAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            10190..11044
                     /locus_tag="PC523_002787"
     CDS             10190..11044
                     /locus_tag="PC523_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476294.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWILDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINAGIIEVKKEYELRLNGIKVEKLDL
                     PSSSIAVVKFHENKVEYFLLGDCTLFIKNGESKVIKDRSICKFDNIVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKDGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKIPRFKVKDDSSC
                     LYLDIYND"
     gene            11087..12322
                     /locus_tag="PC523_002788"
     CDS             11087..12322
                     /locus_tag="PC523_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDF2476295.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSDVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINKLKYQFKINI
                     DRAMNDFKPELIICHHLYLLTAFVRETVKDIKVMSICHGTCLRQLNTIDLEKEYIISN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSRDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELHDFENNLHNAIDSKIQSLLHNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            12424..13365
                     /locus_tag="PC523_002789"
     CDS             12424..13365
                     /locus_tag="PC523_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HDF2476296.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIKGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEEMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLING
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNIIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVA
                     LVSNKSDKSILSGASKVTAIGISDNSIIEQCLDAVKK"
     gene            complement(13582..13935)
                     /locus_tag="PC523_002790"
     CDS             complement(13582..13935)
                     /locus_tag="PC523_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF2476297.1"
                     /translation="MDRKRELKQLYKERKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SIKFQLGAGSCMIKELNNEWKKYGENAFTFEVLELIKHDDNKTKKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
BASE COUNT         5544 a         1410 c         2604 g         4428 t
ORIGIN      
        1 ataaagatac tttaaaatga aatgtaactt aaattaaatt ttagtaggaa ttttataggt
       61 ttatttttaa aggttttttg aaaataaatc tatttaaaaa ttaaattaaa atgcaagaaa
      121 aacataataa ctttcatttt taagaagttt tcacaaaaaa gaaaaaatat agtgcaataa
      181 atgatacaaa gtaatataat agattacaag atatgaaaaa ctaaaaaaat aaattgcaaa
      241 tgaaattaaa atgaataaaa aataaaaaaa taatcataag ttagaggtat caatggaaaa
      301 taagacctaa tgtttttaaa attaaaagta tactaagggg ggcattaata tgtgcgttat
      361 atcaaaagat agagcaaagg aaataaaaat agtagatact actttaagag acggagagca
      421 aacagctggg gttgtatttg caaatagaga aaaaataatg attgctgaaa tgttaagtga
      481 cctaggtgtt gaccaaattg aagttggtat acctactatg ggtggagatg aaaaaaatgt
      541 aataaagcac atatgtagta gaaatttaaa atcagatata atggcttgga atagagcagt
      601 aataaaagat gttgaagaat ctatttcttg tggtgttgat gcagtagcta tatcaatatc
      661 agtatctgat attcatatag aaaataaatt gagaacttct agaggctggg ttttagaaaa
      721 tatggctaaa acagttgaat ttgcaaagaa aaatggacta tatgtttcag taaatggaga
      781 agatgcttca agagcagata tagacttctt aactgaattt ataaatgttg gaaaacaagc
      841 aggggcagat agatttagat attgtgatac agtaggagtt atgaatcctt tttctataaa
      901 gaatgcaata gaaactcttt atgaaagaac taattttgat atagaaatgc atacacataa
      961 tgactttggg atggcaactg caaatgcact tgcaggaata gcagcaggag caaattacgt
     1021 tggtgttaca gtaaatggat taggtgaaag agcaggaaat gcagcattag aagaagtttt
     1081 aatggcactt aagtgtgttt ataaatgcga tttaaataat atagatacta gaaaatttag
     1141 aggaatatgt gagtatgtag cacaggcatc aggaagaatc cttcctacat ggaaaccagt
     1201 agttggggat aatatgttta tacatgaatc tggtatacat gcagatggag ctttaaaaga
     1261 cccacataac tatgaaccat ttgacccaag tgaagtaaat cttgaaagaa aaatagtaat
     1321 aggtaaacat tcaggaagag ctgcagttgt aaataagttg agtgaatatg aaatgtatat
     1381 ttctccagaa aacgctacca aactattaaa tgctataaga gctacatcta taagattaaa
     1441 aagaagttta atggataaag aaattcttca attatattgt gatatactag cacatgagaa
     1501 gggtacaaca gaagaagaag cagttagagg ttcatatatt taagatataa ttgtttagga
     1561 ggaaaatatg ggagataata tagtttataa aataattaaa aaacatatag tagatggaga
     1621 agctgttgct ggaagttcaa ttggtataaa aatagaccaa actttaacac aagattccac
     1681 tggaacaatg acgtatcttc aactagaagc tatggggata gacaaagtaa agactaaaag
     1741 aagtgtggcg tttgtagacc ataatatgtt acaacaagga tttgaaaatg cagatgacca
     1801 taagtatata caaacagtgg cagataaata tggagtttat ttctctaagc caggaaatgg
     1861 tatttgtcat caagtatttt tagaaagatt ttcaacacca ggagatacac ttttaggttc
     1921 tgatagccac acacctactg ctggaggtgt gggaatgatg gctattggag ctggtgggtt
     1981 agatgttgct ttagctatgg caggaggagc atactatata aaagcaccaa aagtctgtaa
     2041 ggtgaatcta gttggaaaat taaataatat ggtatcatca aaagacataa tattagaagt
     2101 attaagaaaa caaactgtaa aaggtggagt aggtaaagtt tatgaatatg gcggagaagg
     2161 tgttaaaagc ctatctgttc cacagagagc tacaataaca aatatggggg ctgaacttgg
     2221 agcaactact tcaatcttcc caagtgatga aaaaacacta gaatttttta agagccaagg
     2281 aagagaagat gcatggatag agttaaaacc agatgcagat gcagtttatg atgaagagat
     2341 tactataaac ctggatgaat taaaaccgtt agctgcaaaa cctcatagtc cagacaatgt
     2401 agatgaagtt gaaaatatag gcaaaattaa aatagaccaa gttgcaatag gaagttgtac
     2461 aaattcttct tatgaagact taatgaaagt agcacaaatc ctaaaaggaa ataaagtaca
     2521 taaggatgtg agtttggtaa tagcaccagg ttcaagacaa gtgatggaga tgattgctag
     2581 aaatggagct ttagcagata taataagtgc tggagctaga atactagaaa attcttgtgg
     2641 tccatgtatc ggtatgggtc aatctccagg tactgactct gtgtctttaa gaactttcaa
     2701 tagaaatttc tatggtagaa gtggtactct atcagcgcaa gtatacttag taagtccaga
     2761 agtagcagca gtaagtgcaa tcaagggagt gcttacagac ccaagagaat ttgatattaa
     2821 atttaccaat ttagatgtaa atgaattttt gatagatgat agcatgataa ttaaacctgc
     2881 tgatgtagga agtgatgtag aagtagttag aggacctaat ataaaacctt tccctcttaa
     2941 tacagagcta agtcagtcta tagatggaaa agttatatta aagacagaag ataatataac
     3001 tactgaccat ataatgccat caaatgctaa gttacttcct tttagaagta atataccgta
     3061 tcttgcaaac tattgtttca acactgtaga tacagaattt cctcagagag caaaagagaa
     3121 taatggtgga tttatagttg gtggagataa ttacggtcaa ggttcaagta gagagcatgc
     3181 agctttagca ccattatatc taggtgttaa aggagtaata gttaagagct ttgcaagaat
     3241 ccacaaggct aacttgataa atagtggaat aattccaatg gagttttgtg atgagaaaga
     3301 ctatgaaaat atatctcttt tagacaattt agagatacca aatattttag acaatctaag
     3361 taatggaatt ttagaagtta agaatacaac taaaggaact tcttttaagg ttaaagtaga
     3421 attatcagca aaagaagttg atgtattaaa agctggtgga aaattaaact atactaaaaa
     3481 tcaagctaat taatatgaaa agagggaata ttgaatgtat aaagtgacac ttataccagg
     3541 agatggaata ggaccagaag ttgcgaaagc tatgaaaaag gtggtagaag ctactggagt
     3601 agagatagaa tgggaagaag ttaatgcagg agaggcagtt attgaagagt atggcactcc
     3661 acttcctgaa tatattatag acagtataaa aaagaataaa atagctataa aaggtccaat
     3721 tactactcct gtaggtaaag gatttagaag tgtaaatgta actttaagac aggcattaga
     3781 tttatatgtc aatttaagac ctattaaaag ttttaagggt ataaaatcaa gatatgaaga
     3841 tgttgattta gtagtggtaa gagaaaatac agaagattta tatgctggaa tagaacataa
     3901 aataggtgat tatgcagctg aaagtataaa gataataaca agaagtgcaa gtgaaagaat
     3961 cgttgatttt gcgtgtaact atgttaaaga taacaagaga aaaaaagtta cagctataca
     4021 taaagcaaat ataatgaaaa tgtctgatgg attattttta gatgtgttta gagaagtagc
     4081 atcaaaacat gatgtagaat atgatgattt gattgtagat gcagcagcta tgaacttagt
     4141 tttaaatcca gaaaattatg atgtaatggt aatgccaaat ctttatggag atatactatc
     4201 agatttaggt gctgggcttg ttggaggttt aggtataatt ccaagtgcaa atattggtaa
     4261 agattgtgcg atatttgaag ctgttcatgg aagtgctcca caaatagcag gtcaaaataa
     4321 agctaatcca acagcactta ttcagtcatc agtaatgatg cttagatact taggcgaata
     4381 tgaaaatgca caaaaaatag aaacagcttt agaaaaagta tttcttgaag gaagcaaatt
     4441 gactgtagat ttaggtggct cagcttcgac tacagagttt gcagatgagg tgtgtaagta
     4501 tattttatag attttgttta tggaaaaagt agaatataga ttaaaagaaa ggggctttct
     4561 caaaatagag agaagcctct ttctttgtta aaatgataaa atgtatttga ttctatgcaa
     4621 aagagagttc ctcataaacg aaaaagttca ttttgggatg ctcactttaa ttttaaaatt
     4681 atttgattaa taggcaaata aaggttaaat aaatttagtg tttttaattg aactttcaac
     4741 caatatatgg taaaatttaa aaaagttgaa ctttcaacta aaaacaaata tattgattat
     4801 gtgaaattct atgaataagg aactctgtga ctttagtcat gttaggttca gtggaggtgt
     4861 gtttgattaa gaccttagat agtaatatat taagagaagt aggtacttta tctagagcag
     4921 taaattcaat aaatgatatt aagtacaagg aattaaagtt acaaaaagga caatttacat
     4981 tcttgacaag aatttgtgaa aatcctggta taaaccttgt ggaattatca aacattttaa
     5041 aggtagacaa ggctacaaca accaaagcta tacaaaagtt aataaaagct ggatatgtgg
     5101 ataaaaagca ggatgagttt gataaaagag ggtataattt aactccaact aataaatctt
     5161 tggaagttta tgagttgata attgaagaag agaatagaag tatagagata tgttttaaga
     5221 attttacaga tgaagaaaaa caagtggtaa ctaaattatt agaaaaaatg agcaaaaatg
     5281 cagaaaatga atggtttaaa gtaaagagat agtaaataaa tacattaacc taaggagata
     5341 gttttatgat tagaaaagcc aatatgaatg atttagaaag tattatgaaa attataaaat
     5401 cgacagtgaa agaaatgaag acttataata atacacaatg ggatgaaaac tatccattag
     5461 aaaaagactt tgtttcagat attaagaaac aggacttata tatatatgaa gtggatggag
     5521 aagtagcagg atttatatgt ctcaattatg aagagccaga agaatatcta ggcttaaatt
     5581 ggtcatctaa taaaaaagca atggtaatac atagaatggc tgtaaatcca aattttagaa
     5641 agaaaggaat agcatctaag ttagttgatt ttgctgaaaa attagctaga gaaaacaatg
     5701 tattatactt aaagagcgat acgtattcaa taaattcaaa aatgaattta ttacttacaa
     5761 agtgtggttt tataaaaatc ggagaaatga gttttttagg aaaggagaaa tctttctact
     5821 gttatgataa aatattataa aaagcattta taatacaagg ggtaaataat aaatttttgg
     5881 tattagtaag ggtataatat aacctagtgt atgccattat gaaagggatg atttatgtga
     5941 ttgaattttt aaagaagaga agaagtatta gaaaatacaa ggatattgaa gtagaaaaag
     6001 aaaaattaga taaaatatta aaagctgctc ttttagctcc atcatcaaag gggcttagaa
     6061 catgggaatt tatagtggta gatgataaag aaaagttaat taaattatct caatgtagaa
     6121 ctaaaggtgg cggatttttc ttaaaaaatg cacctttagc tattgcaatt attgcagata
     6181 aagaaaaaaa tgatgtttgg atagaggatg caagtatagc tgcttcatac atacaacttc
     6241 aagctcatga actaggatta ggttcttgtt ggatacaagt aagaaataga atgcatgatg
     6301 ataatataga agctgacaaa tatattagag aagaattaaa agtacctgac aagtatagtg
     6361 tagaatgtat aatttctata ggatatcccg atgaagaaaa gaaagcatac agtgactctg
     6421 atttagacta tgaaaaagtt cattttaata acttttagta tataatatgt tgtattaaga
     6481 cattatattt ttttatattg ttaatatata cttaaaattg taaaaatata tctgaatttt
     6541 actaacttat ctatgggtta tgtacaagga ttttaaagat aaatatagaa attaatgttt
     6601 ataatcgata cataacttga gtaacaaaat ttaaggaggt taaagtatgg gtatatttaa
     6661 aacaaaaatt gatgaggact ggaaagtaaa ctacattaaa gagtttaatg aaatgagaga
     6721 ttcttatgag agtaaacttc aaaaaaaaca gtttgaagta gatagtttga aatcagaact
     6781 ggatagacta agaagctata aaaatagcct taagccaaaa gaaaaacaaa ttactgatga
     6841 tgatataaat aatataaaga atttaagaag agatggttta agttataaag aaatttcaaa
     6901 tcaaacaagt tggagcaagg caactgtaag tagagtatta aatggacttt acgattagca
     6961 tatacatggg gtgatttaaa ttgggatgaa gtatagttac aaaaaatgta taatagattc
     7021 tattttattg atgcttatag ttcagctatt aaggatgatt ttgaattatg tacttctaag
     7081 tcaatttgaa tttactcttg aaaattttaa tattataaac ttaatttctt ttacactagt
     7141 tggattatca ttaattctat ttttaaaaga taattctttg tataacaagg tgagaaatag
     7201 aaaaatcaca gaagcttttg aagaaaataa aaacagtata cttattgaaa agtgtaagct
     7261 gatacttttt gttgtggttt tatctttagc aataatttca acttattgta ctaagggata
     7321 tgtatttttt aatgtaacta tgatgacttt gtctgtattt atagttccaa tatttgaaga
     7381 gttatttttt agagaataca tatggaatta tctaagtaat tttataaagt caaagattaa
     7441 aattatatgt ataacatcta tattatctgg aatttataat ataggatata tagatattat
     7501 aagaaattat atagcattgt ataataattc aagctacacc tttgaagtta ttatttcaaa
     7561 aattattata ggtactgttt ttggtattgt acttggatta gttagatata ggtttagaga
     7621 tgtaaggctt tgtatactat tgaggagctt atttgctata tttattaggt agataatata
     7681 attagtttat aaggttgttt taaatgtaat tttaaatcaa ccttatttta tttgtgttct
     7741 ataaagtact tatgtagtaa taactaatga atttaataaa aattatttga atatttttaa
     7801 aatatataaa tatgtattga catataaata ttatcaatta caatttatat aaactagtaa
     7861 aaattaaata atattaatca acttattgaa tggaggaata aaaatgaaat ttgtgacttt
     7921 ttgtagtagc aatgaagaaa aaataggtgt atttaattca gaaacaaata gtatatatga
     7981 aattaatagt ttaggattaa gtaaattata tacagatatg aatgatttta ttgaaaacgt
     8041 atctacagga gatttagaaa aaataaaaaa caattcattt gaaaatgcaa agtgttataa
     8101 gttagaagaa gttaaattat gttccccaat tgtaagacct aaaaaagata ttatatgttt
     8161 aggtctaaac tataaagacc atgtcaatga aatcccagat ggagttataa aaaacgtagt
     8221 tatgccagat tatccaattt atttttctaa gagagcagat aaaataattg gagtagatga
     8281 taagataagt ttacatggag acttagtaga aaagctggat tatgaatcag aacttgcagt
     8341 aatcataggt aaagaaggta taaatatatc aaaagaagat gcttatgact atatatttgg
     8401 atatacaata gtgaatgata taagtgaaag agcactccaa gataaacatg ttcaatggtt
     8461 tagaggaaaa agcttagaca cacatacatc tatgggacct tgcatagtac ataaagaaga
     8521 atttgaacag cctttaaaat tagatataag tagtgtagta aatggagaag taagacaaaa
     8581 ttctaataca gaatacttta tatttgatat acctaccata ataaatgatt tatcaagagg
     8641 aatgacttta aaaccaggag atataatttc aactggaact ccagcaggtg ttgctatggg
     8701 tatgaatcct caagtatatt taaagcatgg tgatgtagtt gaatgtaaag tcgaaggaat
     8761 aggtgtttta aaaaatatag ttgattaatg gataaatatg ccatataatt ttattagtac
     8821 gaataattta attaatatag gattaatcaa acttatgatt aatttattat agagagggat
     8881 gagtatatgg caagagaact agaattaaag tatggctgta atccaaatca aaaaccatca
     8941 aagatttata tgaagaatgg agagcttcct atagaagtat taaatggaaa acctggatat
     9001 ataaactttt tagatgcatt taatagttgg caacttgtga aagagttaaa agaagctaca
     9061 ggtcttccag cagcaacttc atttaagcat gtaagtccag caggagccgc agtaggaata
     9121 cctttatcag atactttaaa gcaaatatat tttgttgatg atttagagct ttctccatta
     9181 gcttgtgctt atgcaatggc aagaggagca gatagaatgt cttcttatgg tgattttata
     9241 gcactttcag atgtatgtga taaagagact gcaactatta tagcaagaga ggtttctgat
     9301 ggtataatag ctccaggata tacagatgaa gcccttgaaa tattaaaagg aaagcgtaaa
     9361 ggtaactaca atattgtaaa aatagatgaa aactatactc ctgaacctat tgaaacaaag
     9421 gatgtttatg gaattacttt tgaacaaggc agaaatgaaa tattaataaa tgaagaccta
     9481 ttaaaagata ttccaactga taataaaatt tttacagata atgcaaaacg tgatttaatt
     9541 atagctctta ttacattaaa atatacacaa tcaaattctg tttgttatgc taaagatgga
     9601 caagtaatag gagtaggcgc aggtcaacaa tcacgtattc attgtactag acttgcaggt
     9661 aataaagcag atacttggta tcttcgtcaa catcctaaag tattaaactt aaaattcaaa
     9721 aaggatatag gtcgtccaga tagagacaac actattgatg tatatctttc tgatgattat
     9781 atggatgtgc ttgctgatgg catatggcaa aacttttttg aagaaaaacc agagcctcta
     9841 actggagaag gaaaaagagc atggcttaaa actttaacag gagtagcact tggttcagat
     9901 gcatttttcc catttggtga taatatagag cgtgcaaaaa ggagtggggt aagctttata
     9961 gcacaaccag gtggttctat tcgtgatgat aatgttatat taacttgtaa taagtataac
    10021 atagtcatgg catttactaa aaatagatta ttccatcatt aaaatactga aaatataaag
    10081 ataatataga ttaagtaaaa atctacaaca agttttgttg tagatttttt tatttgctgt
    10141 ataattttaa atagaacttt atttttagat attcaatttg gaggttgata tggctttaaa
    10201 tataagaaac tatagcctat tatgtaatca agggacagct aaatttaatg aagatgtagt
    10261 cggtataaat ccatttggag catggatttt agatggtgct acaggactta atggaaaaaa
    10321 tttagtgtca actgaaagcg atgcaaggtg gtatgttcag tggtggaata ggtatttata
    10381 taaaaatata aataaagaag aatcattaaa gagtattatt aatgctggaa taatagaagt
    10441 aaaaaaagag tatgaactaa gattaaatgg tattaaggtt gaaaaattag atttaccatc
    10501 tagttctata gctgttgtaa aatttcatga aaacaaagta gagtattttt tattaggaga
    10561 ttgtactttg tttattaaaa atggagagtc caaagttata aaagatagaa gtatttgcaa
    10621 atttgataat attgtatttg atgaaatgag caaactttct aacttggaag agatggcatt
    10681 tgatgagatt agaactagtg ttatggatac aatagtaagt aataggttga agaaaaatac
    10741 gaaagatgga tattggatat tggattttga tgaggaagct gtggaaaatt ctatatatgg
    10801 atacattgaa ataaaaaaag attttcaatt gatgctaaca agtgatggtt ttacaagtgc
    10861 atgtgataga tataatctaa taaaagaaga agaattgata cgtatagcag aaaaatttgg
    10921 tatagggtat atacacaata aagtaagaga ttttgaagat aatgactata aagcggttaa
    10981 aatcccaagg tttaaagtaa aagatgattc atcttgttta tacttagata tatataatga
    11041 ttagactgtg taaaactatt taattttaga agggaggtaa gtgaaagtga aaattcttca
    11101 tataataaca caaaaaccaa atagtacagg tagtggtata tatttgtctg gtatgataaa
    11161 aggattcgaa aagataggtc ataagcaagc tgtaattgca ggtatagatg taaatgatga
    11221 tgtaaattgt tttccaagtg atgtaagttt ttatccagta aagtataatt gtggggagtt
    11281 aaattttcct gttgtaggta tgagtgattc tatgccatat gaaagtacaa gatataaaga
    11341 tttaaatata gacatgataa ataaattgaa gtatcaattt aaaataaata tagatagggc
    11401 tatgaatgat tttaaaccag aacttataat atgtcatcat ttgtacctat taactgcatt
    11461 tgttagagaa acagtaaaag acataaaggt catgtctatt tgtcatggaa cttgtttaag
    11521 acagctaaat accattgatt tggagaaaga atacataata tcaaatataa gaaaattaga
    11581 tttaatattt gctcttcatg aaaatcagaa atatgatatt ataaagactt ttggagtaag
    11641 tgaaagtaaa gtagttgtaa ttggtagtgg ttataatgat gatatatttt ataataaaaa
    11701 ttataaaata aaggatgata aaataaaaat agtatttgca ggtaagatat gtaaatcaaa
    11761 gggactaatt ccatttataa aagctatatc taaattaaaa tatagtagag atttaataga
    11821 agtcaatttt gcaggtactg gaagtgatat agaatcatat aatgagatag ttaaactcgc
    11881 aagtaagtct ccatttaaaa tgaatttcct aggaaagtta gagcagagag atttagctga
    11941 gctattcaac aggtcacaaa tatttgtgtt gccatcattt tatgagggat taccagttgt
    12001 agttttagaa gccttatctt gtggaacaga tgtaatcact actgatattc ttggtgttaa
    12061 agagtggatt ggtagtgaga ttaataattc tggaaagata gaatatgttt ctctaccttt
    12121 tatggagaaa gaaggtattc caaaggatga agagttacat gactttgaaa ataatttgca
    12181 taatgcaata gattctaaga tacaatcttt attacataac tcaaataaaa aaacatctgt
    12241 agatatgtca aaaaaaacgt gggatggctt agcctataga ataaatgaag ttattttaat
    12301 ggatgagttg tgtttggttt aaatttattt agattataaa aaagtgtaac tatttttaca
    12361 ctttttttaa atgattttat attatactaa tactaaatat atttgttttt agggaggtaa
    12421 tttatgagga ttagtaaaaa ggtattggcg ttaggtattt cagcattatt gttaagtgtt
    12481 agttttccaa catcaataaa tgcactagat aagattgaaa atattaaagg agtagataaa
    12541 tatgagacag ctgggcttat ttcagataag caagatttta caacagcagt tcttatcaac
    12601 gcggattcta caatggtaga tggtatcgct gccagtggtc ttgcaggagt aaataatgca
    12661 gctattttat taactaataa ggatgatata ccagaagcaa cacttcaaag actgaataga
    12721 gttactaaga tatatgtaat aggtggagaa aattctataa gtaaagatgt agaagaaatg
    12781 ttacttatga gaagaatgca agttataaga attgatggtg tagatagagt agatacaagt
    12841 tataaaattg caggtgaaat agaaaaaatt aaaaacagcg ataagatgtt tttgataaat
    12901 ggatttaagg atgaagctga tgcagtaagt gttgcatctg ttgcttatag agatggagct
    12961 cctataatat tgacaaaaga tataccttca gcagaagaag atacagactt agacccatgg
    13021 tttggagttt ctcctgttcc agtatatgct attggaggag agtctacact aagtgattac
    13081 atagtgagta gatatagagc aactagaatt ggtggtgttg atagatatca aactaataaa
    13141 aatattattg aaaagttcta taatggagca aaagagttct atataacaag tggagacgat
    13201 ttagtttatg ctttggttgc atcaccactt gcaaaaaatg ctcctgttgc tttagtttca
    13261 aataaaagtg ataagagtat attaagtgga gctagtaaag taacagcaat aggtataagt
    13321 gataatagta ttatagagca gtgtttggat gctgtaaaaa agtaatagag tgtcattagt
    13381 aaaatgaata tataccattg aaaattagta gatagttcat tttataatgg tataaaatat
    13441 atgagaagag gctatcaaat agagataagc ctctttttca ttgttaaaat tatgaaaata
    13501 tatttatttc ataaaaaagt atcacatgaa ctaaatgtct attttgagac actatcttat
    13561 tttcttagat tagatgattt tttatatgga ataaatttca taagtattat cttccttcaa
    13621 tttttccaac caaaccatct ctaaaatatc tagttcttct aaatagtctt tctttgtttt
    13681 gttatcatca tgttttataa gttctaaaac ttcaaatgta aatgcatttt caccatattt
    13741 tttccactca ttatttaact cttttatcat acatgaacca gcaccaagct gaaatttgat
    13801 agagttaaat ttgcttttta tgtcattact ttttccaagg taaatctttt tagtaatatc
    13861 attttttatt atgaatacac ctgtgtcaaa tttcctttct ttgtataatt gttttaattc
    13921 acgttttcta tccatgattt tatatacctc tctttataat taatatgtaa tatgtaaata
    13981 taaaaa
//
LOCUS       DAMJVM010000090        13618 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.094, whole genome shotgun sequence.
ACCESSION   DAMJVM010000090 DAMJVM010000000
VERSION     DAMJVM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13618
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.094"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(230..1897)
                     /locus_tag="PC523_002791"
     CDS             complement(230..1897)
                     /locus_tag="PC523_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HDF2476298.1"
                     /translation="MKKINLKIEGKDKEYSLEENSHGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEVDVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDNNKRVILIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLED
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            complement(1971..2726)
                     /locus_tag="PC523_002792"
     CDS             complement(1971..2726)
                     /locus_tag="PC523_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDF2476299.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSDLCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELRGFDIRYFNLHLGYV
                     MTNRVRRNRDKYLENSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDDYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLDNIVGEG"
     gene            complement(2736..3272)
                     /gene="lepB"
                     /locus_tag="PC523_002793"
     CDS             complement(2736..3272)
                     /gene="lepB"
                     /locus_tag="PC523_002793"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2476300.1"
                     /translation="MNETIKEEIVEWIKIIITALFLAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            complement(3438..3974)
                     /gene="lepB"
                     /locus_tag="PC523_002794"
     CDS             complement(3438..3974)
                     /gene="lepB"
                     /locus_tag="PC523_002794"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2476301.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDVDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHAI
                     LGKVLVRLYPFSKIGTID"
     gene            complement(4074..5384)
                     /locus_tag="PC523_002795"
     CDS             complement(4074..5384)
                     /locus_tag="PC523_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDF2476302.1"
                     /translation="MTKLPKEFLEDVEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLGLFDLNQISWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINVSRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSHAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRRENLYLLPK
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKETSGVIKNHYPKGLRKVF"
     gene            complement(5463..6179)
                     /locus_tag="PC523_002796"
     CDS             complement(5463..6179)
                     /locus_tag="PC523_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HDF2476303.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVILLWKINLDKLE
                     KYMNNSKKVFFGNLRKKKKK"
     gene            6416..7687
                     /gene="sleC"
                     /locus_tag="PC523_002797"
     CDS             6416..7687
                     /gene="sleC"
                     /locus_tag="PC523_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HDF2476304.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTTYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRFFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVRIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            complement(8161..8817)
                     /locus_tag="PC523_002798"
     CDS             complement(8161..8817)
                     /locus_tag="PC523_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476305.1"
                     /translation="MKKGDFIWSGILICIIAVLAIPSSREVFMEATGAHPYIGGFFKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGIVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(10018..10428)
                     /locus_tag="PC523_002799"
     CDS             complement(10018..10428)
                     /locus_tag="PC523_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476306.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKLESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEKDL
                     EKIKTEGLKIMKPITIKNEDGSQISIAYNYAAID"
     gene            complement(10644..12002)
                     /locus_tag="PC523_002800"
     CDS             complement(10644..12002)
                     /locus_tag="PC523_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2476307.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNASTENYTLKKAGDSTGKNNSDKFEIMSSIE
                     NIMMLEMLGILSFIVTIIFSILLCFRYIKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGIGLVFATENKEINEVESF
                     GLTKNEKSVLSISSDSTELYTPKVDSNISVSMSKVSMNKSSESKSYEDISMNKVRNTE
                     NYSEVSVEKSSKSNDYEDVLYTPSAEYSIQKNNYNEGVLVISRKGNLLAVNNIQYRTS
                     IK"
     gene            complement(12013..12372)
                     /locus_tag="PC523_002801"
     CDS             complement(12013..12372)
                     /locus_tag="PC523_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2476308.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(12577..13356)
                     /locus_tag="PC523_002802"
     CDS             complement(12577..13356)
                     /locus_tag="PC523_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDF2476309.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKMGDIFVNGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICENKDNLGVFKSMSASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         4650 a         2440 c         1328 g         5200 t
ORIGIN      
        1 ttactctttt aaaaaattat cacacaaatt aacaaaatat taaacaaaga agtaaacttt
       61 aaaataaaaa gcttgcttca tacaaaagta taaaacaagc ctttttatat atcaactcaa
      121 actttaaaat ctgtatcaat ttaattttcc atttaatata cttcacataa accaatcgca
      181 ctatttacta tgccatttaa tcttcataaa ataatctata atacacatat taatcaacta
      241 ttttacttcc tccaataaat tccctaagaa ttgaaatacc tggtatatca gaagtgtcgt
      301 ccaattcaac aaaatactgc aaaaacttta gtaatctagt agcttcaata tgtgcaggat
      361 tatcttcctt tatatcttcc aacaaaggtt tcgcaaatcg ttttaaaact gccaattcat
      421 atacacaagc agagttaaat atagcatctg cctcttcttg gtatgggaat atattcttct
      481 tttctcctct tctaactgat gcccattgta aaatagtatt atcagcacta tatcctctaa
      541 aattatagtc tctaacaatt cttcttatca ttctcaaatc agcagtaggg attcgattat
      601 ggtcatccaa atttatttga gtaagggcac ttatgtatat cttaaattta tcttcatctg
      661 gtatagatga tgttaaaata ggatttagcc catgtatacc ttctaatata attggctgat
      721 ttgaatctat ctttattttt ttaccagtat actctctctt gccttcttta aaattgaatc
      781 ttatatcatc aatttcttca ccagctaaaa gtttctttaa gtctaagtta aacttctcta
      841 aatctattgc ataaattgat tcaaaatcat aatttccata ttcatctaaa ggggtatgtt
      901 ttctatctat aaaataatca tctaaagaaa tcgaaatagg gtttaaatta ttcactctaa
      961 gatgtattga taatctgtga gcaaaacttg tttttccaga tgaagatgga gctgctatta
     1021 aaattactct cttattgtta tcctttatca tatctgctat ttgagataac ttcttttcat
     1081 gcaatgcttc aaatgttctt attatatctc catattcatt gttttctatt atcttattaa
     1141 gagatactac cttgtcaatt cccataagtc ttgaccattc ttctgcttca taatatacac
     1201 ttgatagctt aggttgaggt acaaatttca taggaagagt cttatcttct tcactaggac
     1261 ctaaaattat aacacctcca ttataaagct taatatcaaa tgattttata taaccagttg
     1321 atggcaacat ataaccataa aaatggtcta tatagtttct acacttatat actttagcac
     1381 tatcatactc tttgtatttc aaaagctctg ccttttcata catttcatta ttttcaaaga
     1441 tagaaatagc ttcatttctt gtaacttcaa ttttttctat aacataatcg ttgtttacta
     1501 tctctttcat cttgtttttt atattttcaa catccacttc tttaagcttt ctatctatat
     1561 gaacttcaca atatagccca tctgataacg aatgttctat tgtaactcta ctatcccaaa
     1621 agatttctct acaagccatt acaaatataa acgacatgac tctaaagtaa actctctctc
     1681 catcttcatt agtagtatct aaaaaattta tctcacaatc cttcttaact ctacaattta
     1741 gctcttttag tttattgtct acaactgcaa gtgttatata acccttatgc tcatcacaaa
     1801 attctttggc tatatctccc aatctgatac catgggaatt ttcttctaat gaatattcct
     1861 tatcttttcc ttctattttt aagtttattt ttttcacttc aacatctcct tccttttgat
     1921 gaatataaat tttaattaat gaggcatatc aacaagataa agagaggtat ttaaccctct
     1981 cctacaatat tatccaattt acttatagta tcttcaatat cttcgtaatt tatttctaat
     2041 actaaatatt ctgcatctag tgttaagacc tctttaatcc cctcttcaga taaaattcct
     2101 cttcctatac caacatgtgt atcattgata ccatcattat cacttaagtg tatcagcctt
     2161 attttatcct ttaagttctt tacataatca tcctcatcaa ttaaacagtg tccagtatcg
     2221 taacaaaaac aaatcttaga atttttaatc ctcttaaata tatattcaaa atcataagat
     2281 atatttccaa tatttgaaaa atccccacct tttgaataag tattctctat acatacatat
     2341 gaatttgtat ttagtttgtc taaaaaatcc acactatttt ctaaatattt gtctctattc
     2401 cttctaactc tatttgtcat aacatatccc aaatgaaggt taaaatacct tatatcaaat
     2461 cctcttagtt caaattctat cttctcaata aaatttatcc atgaatttcg tatatactct
     2521 atgttttcgc aagtatttaa ctccataggc aagtgtatcc ctatagataa tcctaactta
     2581 gaaattctct ctttatattt acatagatca ctacattcag atatattgtc aattccaatc
     2641 tctaaatgag ttatttgctt tatactctca caaatgttta aagcctcctc aatattaaac
     2701 aataaggatg ctataccaac tgccatacaa atcacctact caaaaatacc tatatctgta
     2761 aaaggaaata ctcttaaaat aacttttcct ttaatatttt cttcatctac taatcccact
     2821 tctttatatc tactgtctaa actaacttct ctattatctc ccattgcaaa tacttttccc
     2881 ttaggaatca ccatatcaat atcgccttca gtataattat catgtatata tgaaacttca
     2941 tttaataact taccatttat ataaacctta gaatccttaa tttttagatg gtcaccagga
     3001 acacctataa ccctttttac aagctctttt ttccttccat tctcttgtaa taaatcagtc
     3061 ttaaatatca ttatatctcc acattttggt tctgataact tatatgtcat cctgtttgct
     3121 accaaataat catgtgattt aagtgttggg tacattgatt ctccatttac tagtgttggt
     3181 tttataaaac gagttataat aaatgccaaa aaaagtgcag taataattat ttttatccac
     3241 tctacaatct cttctttaat agtttcattc attcacaaat tctcctttat agttatatta
     3301 tctttattaa taatgtctct taaacactat cttttagata agtctactaa ataaggtgtt
     3361 gtctttttta tatagacaac tattgtgtat ggtctggtga tatcagttaa tcagcgattg
     3421 ctccaaattt tatatgttta gtcaatagtc cctatcttag aaaatggata caatctgact
     3481 agaacctttc ctaaaatggc atgctcgtcg accaatccaa cctcatcgta tctactatct
     3541 aaactttttt ctctattatc tcccattgca aataattttc cttctggaac tacgatatca
     3601 acatctccat cagtacgatt attatgtata taggaagttt catctaacaa tttatcattt
     3661 acatatacct tggagtcttg tatttttaca tggtcaccag gaaccccgat aactcttttt
     3721 actaaatctt ttttctttcc attttcctct gttaagtcgg ttttgaatac tattatatct
     3781 ttgtattttg gctctcctac cttgtacgca attctattaa ctatcaaata atcacgttca
     3841 accaatgtag gatacattga ttctcctttg actattgttg gcactataaa acgagttatt
     3901 gcaaatgcca aaacaagagc tatgacaatc acttttatcc attctacaac ttctttttta
     3961 actgcttcac ccactaaaat cgtctcctct gtattaaata ttaagtcaat ctacagcaaa
     4021 ataatctact ataaacctac aatataaata ttatacttct tttttctact agtttaaaat
     4081 acttttctaa gacctttagg ataatgattc ttaattacac cagaagtttc ttttccccaa
     4141 cctaaaggta tgccttcaac tgaaaccaaa acccatcctc tactatcatt agaatttaca
     4201 acatcccctt ttaaatattt aagaattgat tcactctgta cactgaagtt ttcaacattt
     4261 tttacatctt caacttttaa gtaatgagat agtgcatgag aaggctcaaa tctattcttc
     4321 tttaaaaccc ctaaatgcaa accgtatctc aatactttta gtttttttgt atctggatat
     4381 attttaggaa gtaggtacaa attttctctt cttaaataaa atttattttt tttattacta
     4441 taaaaatcaa cctttaaaaa tttcttttca aactccctat aatcctttat ttctttatct
     4501 aaatttttta tttttaattc tttgacatta cattcttctt cttctaattt ttttattttg
     4561 gcaacaaaat gcccttctcc ttttatctta tgaggccata atctttccat ttcaataagt
     4621 tctgcatgtg gatattcact gataaattca tttataacat gctcattttc ttctcttgaa
     4681 aaagtacaag ttgagtaaat taaaatacca tccttcttta acattttata tccatctctt
     4741 ataatctctt tttggatgga ctgacactct actacctttg catgactcca atctgatata
     4801 gcaacctcgt ccttcctaaa cataccttgc ccagaacaag gtgcatctat aataacctta
     4861 tcaaaatacc cattaaacgt ctttcttaag ctattagaat cagtgtttgt tataatacaa
     4921 tttctcgctc caaatcgctc tagattttcc cctagagcct taattctact aacattaatt
     4981 tcattagaaa ccaataaacc tgtatcattt agcttagata gtatatatgt agattttcca
     5041 ccaggagcag cacacatatc gagaactttt tcaccttctt taatatctgc ctttggtatt
     5101 acactcattg cagaaggctc ttgcaaatag tatgctccag cctcatgcag tggatttcta
     5161 ccaggtctat caattttatc atcataataa aaaccttctt ctgcccatga tatttgattc
     5221 aaatcaaaca gacccaaatc aaataatttt ttcttgtcaa tttttagagt atttattcta
     5281 agcccagtag tcttactttc ttcataactt tttataaaat catcatattc atcttttagt
     5341 atttcttcaa catcttctaa aaattctttt ggtaactttg tcaaatctat aatccccctt
     5401 caatctgctt tacttgtgtg tcatttatta tactttaatt atttatttct ttctcttaaa
     5461 ttttatttct tcttcttttt cctaagatta ccgaaaaaca cttttttaga gttattcata
     5521 tatttctcaa gtttatcaag atttatcttc cacaaaagta ttacataaac tataagtaat
     5581 ataatagccc atctatattc tatcatcaat atgaaatcaa aaactccatg taataaaatt
     5641 ggaattaaca ctgccataaa caaatactca cgtcttttat ttttatttcc ctcaaattta
     5701 tacttagaaa tatagtaacc catagtaatt gcaaacatta catgagctgg tatagagatg
     5761 actgctctat taataccaac ttgcagtgct agatttctac tttcagaaaa aatataaacc
     5821 atattttcaa ttgttgcaaa tcctaaagct aaaaatacag agtaaataat accatctaac
     5881 ttttcagtaa aatgtttttc tttaattaag gcaggaatca gtatcagagc ttttaccccc
     5941 tcttctacaa gtcctgcaac aacaaatgat acatatatta gttctgaata accttcaaat
     6001 acattaaact tcatcagtaa attttctaaa agtataatta taacacttag aaaagcaccg
     6061 attaaaaaaa atttaattag atgtattaga ggttctctac aatatctatc tttagacctt
     6121 atccaaaaca ttcctatcaa tataggaact attgctaata aaaataaatc tagcttcata
     6181 gattattttc ctttcaaaaa ttttgattta tttatgactt atatcatcta cttattttta
     6241 acttatttat gttttaaata cacactaaaa tatacattaa attattttaa attatttaaa
     6301 tacaaatata ctcaaaaaaa tttaaattta tatctatatt atttaacaaa tttcaagcta
     6361 tattcatatc ttgaaatata agggaactaa attcatttaa agaaagggtg atttgatgca
     6421 agatggtttc ttaacagtaa gcataattga tgctactaac aatagaccta ttcaaaatgc
     6481 agtagtaaac atatattcaa tgtctaatgg tagccaaagc tcatcaactt tataccaaaa
     6541 cctaagaagt aatgaatctg gtcaagtgac tgggttagta ttaccagcac cagatgtaga
     6601 ttactctcta caaccatctg atgttagacc ttacagccag tatatagtgg aagccatagc
     6661 agatggatat gagacagttg tcatagaggg aacacagtta ttagctacaa ttgaagcaag
     6721 acaaggagtt cccatgtctc ctagaacaag aagtaaacgt tctttctcaa gacaaagtga
     6781 gttgattttt gacataggag aacatacttt atatggtact tatcctccta agattccaga
     6841 aagtaactta aaaccattac cacctcctac tggtttcgtt gtattagata accctgtagt
     6901 gcctgaattt atagtagttc acgatggttt accagaagat tcatcagctc caaactattg
     6961 gattccattt aaagagtaca ttaaaaacat agcatcatct gaaatatatt caacttggcc
     7021 tgagcagaca atctatgcaa atgtaatagc tataatatca tttactttaa atagagtctt
     7081 tactgaatgg tatagaaata aaggatacaa ctttacaata acttcaacaa caacatatga
     7141 ccataaattt ataaataata gaaacttatt tgaaccaatt aacgtagtcg tagatgctat
     7201 ttttaatacc tttataaaaa gacctcctac atcaagacag ccactattag cacaatattg
     7261 tgatggacaa aaatcccaat gtcctgacca aatgactcaa tggggcagta aagatttagg
     7321 tgaccaagga tatgactatg agagtatact aagatttttc tatggagatg agatagtgtt
     7381 tgaaagagca cctatagtaa gtggtgtacc agtatcattc cctggtacta cactacaagt
     7441 tggttcttct gggcagtatg ttagaactat acaaaatcag ttaaatgcca tatctaattc
     7501 ttatccagct gtcccaaaag ttatagaaga tggtatctat ggaacagata cagaaaatgc
     7561 tgttaggata ttccaaggca tctttggttt acctcaaagc ggagtagtag attttaaaac
     7621 atggtatgaa atatccaggg tttatgtagc tacaactaga atagcttctt taaatccttt
     7681 aatttaaagc ttgatttaga atataaactc aaattattaa ataaaattat taacttaaaa
     7741 taaaaatggc tgttatgaac taacatatac cataagtatt tttacttata agtattgtag
     7801 tcttaacagc cattttttag ttataactta tttttttaga accttaaaac tttaaatggg
     7861 ggattttaag cttcttaaaa acagtgctta gcaactttaa tttctttaaa aatcttatct
     7921 ttatttttaa atttaataaa atgctatttt aatgaaaagc ttacttttta cttagagcct
     7981 caattcttat ttaaaatgct acttctcatt tagaatatta ctccttatta tttaaaacat
     8041 tatttctcac ttagaatatt acttcccatc taaattatta tttattcatt ataaatataa
     8101 acactattaa tttaaaaagt tattcctaat tttaataata ccatttcctt atcaatagac
     8161 ctaaaccgca actttagact tattagctaa agccaatatt agacctaaag ctatagatgc
     8221 aaatgcagaa gcaattactc tatactcagc agggaataag aatacaattg tatgacatgg
     8281 tatccagaaa aatggtatag tttttaaaac actaaaacca agataagcat tccagtccac
     8341 aatatttacc acatctttaa gttttacctt aatacccttt ccaacttcat attttttatc
     8401 tataaaacaa tccataacct tatggaataa aaatacagtt ggtgacatta gtatattcat
     8461 agttgcgctc ttaaatagag catttgcaaa agctattccc tcaaatggaa gcataccatc
     8521 cacttgtgct tgacctacac ctgatgagta aattgacatt ccatatgtgg taactatacc
     8581 tattattccc caaactattg ctctataaaa tactcctact ggtattttcc agtccttata
     8641 caaaattcta gcaccaagta aatctcccat tgtagctaat acagcaaact taaaaaaacc
     8701 tccaatatat ggatgtgcac ctgttgcttc cataaatact tctctactac ttggtattgc
     8761 taagacagca ataatgcaaa taagaattcc actccatata aaatcgcctt ttttcatatt
     8821 ataaatacta tttaatatat aaatagttct cctttctatt aaagatttat ttaatttaca
     8881 atctatctac aattatttat taatcactga ttattagata ttcattttcc atgtttaaat
     8941 ttattctagc cctttatcta tttattttct tacttttttt atccaccact atctatatat
     9001 ttattttacg attactcttt caccctaacc ctttaactat ctatttgttt tttactactc
     9061 ttatctacca gtatctatat atctacttta caagtactct ttcaccctaa ctctttaact
     9121 atctatttgt tttttactat tcttatctat cagtatctat atatctactt tacaagtact
     9181 ctttcaccct aactctttaa ctatctattt gtttttttac tattcttatc taccagtatc
     9241 tatatctact ttacaagtac tctttcaccc taactcttta actatctatt tgttttttac
     9301 tattcttatc taccagtatc tatatatcta ctttacaagt actcttttaa cccaacctcc
     9361 tatactttat atagtaatat gtttttaact tttttcatta ttctcaatat gttttataac
     9421 tacaaatact ctccctcctt aaaaataaga ataaaattaa ccaacaaatt tataatcata
     9481 ttattacttt atgtattaag caattactat gccaataaat tttaattttt ttaaacaact
     9541 cttaaaaatg ctaaaatgct aattttcttt aacttttaat gaatctaata gataacattt
     9601 aatttttgtt atatttttca gaatgtttca attattattt ttaagataaa aaaataatgt
     9661 taacatttct taacatacat taaaaaatgt taacattata aattgggttt aatactttta
     9721 ctttgactcg atttactttt attctacttc tttactttat tttaacttag actttatttt
     9781 tttatcactt atttgagtct tatttatttt attctcattc tatatgttca attaaatttg
     9841 tctaagttct ttgatatttt tattacataa ttttaacaag cttaagtgtt atttgggtag
     9901 tgcaattgat aattatttaa ataatcatca attgcatgtt tgtatcatca tagtagcttc
     9961 atgtatttaa tcatgtttac ttaggatttt gaaatatctt agagattata tataacatta
    10021 gtcaattgct gcataattgt aagcaattga aatttgagac ccatcttcat tttttatagt
    10081 gataggcttc ataattttta atccttcagt ttttatcttt tctaaatctt tttccatttt
    10141 cttaattgtt tcctcaagat ctttttgact tacatcttta accgcattag ggtctttagc
    10201 ataatctttt aattcttgaa tatgtttagc atattcttca taagtataat gctcaacacc
    10261 atatttttct aattcagtag ggtcagttat tcctgatttt attagagatt caagtttagg
    10321 taaagcaata ttagatgaat catttccatt tgcaaatact gctccagttc ctcctagtac
    10381 cattgcagat accatcattg cacttataag ggattttttt attttcataa ccaatcttcc
    10441 tttcttatat atttatttgg atagtgtagt ttaaaacgac tattgcaata gttatcaacc
    10501 acacatttag actaccacaa tagtcatatt tatgtcaagt atttcactaa aattttttaa
    10561 taaaaaatac tacctcaaaa caaacttaat tattttaaga tagtatttta attctaattg
    10621 ctttgtgcta ttatgatttg cacttacttt atagaagttc tgtattgtat attattaact
    10681 gctaacaaat ttccttttct tgatataact aaaacaccct cattataatt atttttctga
    10741 attgaatatt ctgctgaagg agtataaagt acatcttcat aatcattaga tttgcttgat
    10801 ttctctacag atacttctga ataattctca gtattcctta ctttattcat agatatatct
    10861 tcataacttt tagattcact tgatttattc atagatactt ttgacatact aacagagata
    10921 tttgaatcta cttttggagt atataactca gttgagtcac tacttattga aagtacactt
    10981 ttctcatttt ttgttaaacc aaaagattca acctcattaa tctctttatt ttctgttgca
    11041 aaaacaagac ctatcccaca aactaataca gttgaaacta aaaaagtaac ataagttgat
    11101 tttttaaatt tcataatatt aactattctc ttttctattt ttgtctttcc aaacccacta
    11161 catattgtca ttgaaaatgg attattctcc tctgctaaac ttattagtgt agttgcataa
    11221 tcagccctat attcctcacc aagtatactc ataacctttt catcacaact aaattctatg
    11281 tctttattta gtaaaaaaaa catcacccaa actaagggat taaaccaatg aacaacaaga
    11341 gctacaagca taaacagctt caacagatta tcaaaactat taatatgaac catttcatga
    11401 gttaaaacaa agtctaaagt ttgattatta gcataattca tttgcttcgg caaaataatt
    11461 cgtggtttta aaataccaga tgtaagtggc gttctagtaa attcagatat acaaacaata
    11521 aatcttctaa aacggctatt cttacacatc cattttttaa tgaactcgtg attttttata
    11581 tgaattgaag ttttaaaaag attaattatt ttaatatatc taaaacataa aagtatgcta
    11641 aatataatag taactataaa agacaaaatg cccaacattt ccaacatcat aatattttca
    11701 atactagaca ttatttcaaa tttgtcagaa ttgtttttac ctgtagagtc accagctttc
    11761 ttaagagtat aattttccgt tgatgcattc cccaattcta catcaaaagt cgattcaatt
    11821 atactataaa tactaaaacc agaatttaaa cttactggta ttagaagttt aaataaaaca
    11881 atcaaccaca ataatacaaa caccttttta ggtaattttt taaataaaca tgctcttata
    11941 aaaataacta ttaaaagtaa tactccagaa gtaaaactca tttctaacaa tttaatgaac
    12001 ataaatctta cctcacttta atttttcaac aatctgttta agttttgtaa tctcatcact
    12061 tgaaagattt tctttgttaa taaaagctga aagaaatttt tctgaagacc ctccaaacat
    12121 tttattaatt aaagtttctg tttcctgttt ttgtacttct tccttgctta ttaaagcttt
    12181 acaaacaaaa ttcggctcaa ttctctctat tgcacctttt gacaccaatt tttttataac
    12241 cgtatacgta gtgtttctat tccatcctgt ttcttctttt aaaattttta ctaactctcc
    12301 agccttgctt tctcctctct cccatagaac ttccattact ttaagttctg aatcaaataa
    12361 ttttatctcc atataaaatc gctcctcaaa tatagttatc acaatagtca ttactataat
    12421 aaaaaactat catagtagtc tatgctatgt caatccttta ttttttaata ataaaaactg
    12481 ccttttaaaa aatttcttat aaaagacagt tttatcaatg ttatactttg atgtttaatc
    12541 aaagccatat tttatttgaa tgtataacta tctatcttaa tacatagtat aagatttttc
    12601 tttatctaat agtatatcct ctacgtctgg gttttctttt actaattgtc tataaaagtt
    12661 acaattagta acacctacat atggaattaa ccatagtaga cctattccaa gagtagctat
    12721 tgaaagtata taccatccta taaaactaaa gtttagtaca aataatttaa gtacatttcc
    12781 tttcatcata tctaaactag cactcattga tttaaatacg cccagattat ccttgttttc
    12841 acaaattagg aaagtagctt gagaaacata taatagcaat atataaactg gtaacataag
    12901 aacgagtatt ataagaacta gagttattcc agtagaaata atagttccta ttccaacatt
    12961 gtcacttgat aaatattcaa ttgcaccact agtaagtaaa actccaaatg ataaaaatgc
    13021 tcctataatt gtgattataa aagaataaat ataaacaata attgattcaa ataaataaaa
    13081 accaaaactt cttaaataaa ttctcccatt tacaaaaata tctcccatct tgactttttc
    13141 atctttagta attttcaaga acatacatgt gaatcctgct gaaacacaga aagctattac
    13201 agccactaca atagtagtca atggttcaaa aggtaatcct acaattgata ttaacactat
    13261 caatgcaata tatactgcca tagaaattgc acatataccc cagtttccag acaactgagc
    13321 tttagatact ctttttaatt cttgccttga taccataaaa aacacccctt tcattttata
    13381 aaaaaacttt taaaaatata aataatcttt taacatataa tattaatata tcatattttc
    13441 gactatataa catacaatta ttcaccatgt tttatcaaga tatgtcataa ttgtgttact
    13501 agttttatat agtcaatatt accaagtaca tcaaataaat ttgactaatt atacgacaaa
    13561 taataaaata cattatacta attttacttt atttaataaa caaattatcc aataaaat
//
LOCUS       DAMJVM010000091        13584 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.095, whole genome shotgun sequence.
ACCESSION   DAMJVM010000091 DAMJVM010000000
VERSION     DAMJVM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13584
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.095"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            268..1197
                     /gene="glsA"
                     /locus_tag="PC523_002803"
     CDS             268..1197
                     /gene="glsA"
                     /locus_tag="PC523_002803"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDF2476310.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            1295..1858
                     /locus_tag="PC523_002804"
     CDS             1295..1858
                     /locus_tag="PC523_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2476311.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            2341..3177
                     /locus_tag="PC523_002805"
     CDS             2341..3177
                     /locus_tag="PC523_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDF2476312.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     NEKDIIKFQEIRKANNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIDRLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            3460..4455
                     /locus_tag="PC523_002806"
     CDS             3460..4455
                     /locus_tag="PC523_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2476313.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADDVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(4689..5093)
                     /locus_tag="PC523_002807"
     CDS             complement(4689..5093)
                     /locus_tag="PC523_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDF2476314.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYNISYNIFEK"
     gene            5851..6765
                     /gene="queG"
                     /locus_tag="PC523_002808"
     CDS             5851..6765
                     /gene="queG"
                     /locus_tag="PC523_002808"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDF2476315.1"
                     /translation="MNNRELKEFCNLIGLNCVGVAGVDKYDNLENILKDRYKKGYLTG
                     MEEPIIENRINPRNIMEDANSIIVCAFPYYINRQEKNKNSNLSKYCHGKDYHIVVKKF
                     LQQICDYISKKVTDFKYKIFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSQE
                     EKAVLKSGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGKNIIKRNMEIILDKYND"
     gene            6871..9108
                     /locus_tag="PC523_002809"
     CDS             6871..9108
                     /locus_tag="PC523_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2476316.1"
                     /translation="MKYNNTSEINPLDCILGQERAVEAMELGLKINNPAYNIYIAGES
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQDYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEGNEEGIARFIASQCDKNNLRHFTKSAVEEVVKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVPIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVQDGKFKIYTVERVEEAIEILTDVKFEEIRKLVKEKLISFSKIQ
                     TVSKE"
     gene            9306..10163
                     /gene="nth"
                     /locus_tag="PC523_002810"
     CDS             9306..10163
                     /gene="nth"
                     /locus_tag="PC523_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDF2476317.1"
                     /translation="MKNVNLKEINKKEVIDKEEINKKGIDKKDVTEKEKINKKSEHDK
                     KTKNSSSSKDTQKLSKGIKSKSKIKNESANEKDVNKILDELEKLYPDAKCELNYGTAF
                     ELLIATILSAQCTDVRVNKVTSELFKKYNTAKDFANLSIEEISKEIKSCGLYKSKSQK
                     IKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVFRVSN
                     RIGIVDEPNPQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASCPIKEDCN
                     YYKELNETK"
     gene            10357..10827
                     /gene="trmL"
                     /locus_tag="PC523_002811"
     CDS             10357..10827
                     /gene="trmL"
                     /locus_tag="PC523_002811"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDF2476318.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPETLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            10862..11710
                     /locus_tag="PC523_002812"
     CDS             10862..11710
                     /locus_tag="PC523_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2476319.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEKEYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEGREILYIAGSATATGTYQSAVM
                     AKNDVEGKIYTYDSNLLCMGTGLLVIKAGELASEGKTVDEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMEDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            11998..13002
                     /locus_tag="PC523_002813"
     CDS             11998..13002
                     /locus_tag="PC523_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2476320.1"
                     /translation="MRFKRILIFILTVAMTAGMLVGCDSNQSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFETGFKEGVKFSNSSIKDISVEYADVFKDRK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIGAVRTKNKKVIATNKDQHELAPENVISSI
                     VKDFENSTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVSKNNK
                     "
     gene            13128..13463
                     /locus_tag="PC523_002814"
                     /pseudo
     CDS             13128..13463
                     /locus_tag="PC523_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
BASE COUNT         5502 a         1270 c         2542 g         4270 t
ORIGIN      
        1 attgagttag aaaattttgg tgttataaaa atttaaatta ttggattatt agattactga
       61 attgctaaat tactaggttg ttaaattact aaattattga attactagat tactgaatta
      121 ttaagccatt taaactattt aaattgtttg aaaaatgaat tgattcttgt agaaaataac
      181 tttaaagtat aatttttttt aacactgttt tattatagta caaacttgtg ctaaaataga
      241 atatataaca taatataaga ggtgcatatg aatacgttag atgagacatt attaaaagaa
      301 attataagtt ctaataagaa gtatactaat tatggacaag ttgctagtta cataccagaa
      361 ttaaaaaacg ctagaagaaa tgatttagga atttgtatca tagatagcga aaataatctt
      421 tatagtgctg gaaactgtag cacaaagttt actatacaaa gtatttcaaa accaattgtg
      481 cttgctatgg cacttatgga caatgattgg gaagacgttt tttcaaacgt tggaatggaa
      541 ccaagtggag acccatttaa ttcaataatg aaacttgaaa ttaatgacac aaaaaaaccc
      601 tgtaatccaa tgataaatgc aggggctata gtaactactt cccttataaa tgggagttgt
      661 ttagaagaaa aagaagaaag aatgcttagt ttctttagaa agttagcaaa aaatgataat
      721 ataggaataa actatgatgt ctataagtct gaaaaaatga ctggagatag aaacagagct
      781 atggcttact tattgaaaag tgatggattt atcagaggta atgtagaaga tgttctagac
      841 ctttatttta aacaatgctc aattgagata gattctgtag atttagcgag aattggaata
      901 aatcttgcaa attatggagt ggatatagaa aatggcgagc atttaatgag tgaaatggtt
      961 tctagaattg tcaagacatt tatgatgact tgtggaatgt atgatgcttc tggggaattt
     1021 gctattaaag ttggcattcc tgcaaaatct ggagttgggg gtggtataat ggcttctgtg
     1081 ccgggacgca tggggattgg tgtttatgga cctgcgcttg acaaaaaagg taacagcgtc
     1141 gcaggagtta aagttttaga ggagttgtca aataaactca aattaaatat tttttaaaat
     1201 attaagaaag gatttttttg acaacaacat ataatttaat atataattaa attgtaaaga
     1261 aatatgaatt aaataaattt tttaggaggg gtcaatgaaa gaggtattgg tattacgaga
     1321 tttggagtgc ataaaggcta ttgcgcatcc acgtagaata gatattctaa aagcatttga
     1381 caaatcgcca ttgtcagcaa aacaattatc tcaattattg gaagaacctc atgcaaaaat
     1441 aaactaccat ataaaaatgt tatacaaagt tggtatttta gaattagttg aggaaaaaat
     1501 aaagtcgggg attgtggaaa agtactatta tccaagtgca aaaaatgtgg ttatagggaa
     1561 taggatactt aatttttctt tagacaatgg ggaagaaaaa gaagaattgt acatatcaaa
     1621 atttgaaaat atgagtgaag ttttttataa ggcagcagag gaagatgttt tagaaaatga
     1681 aaatatagtt gattatcatg atatttctct tactcatgat gaattagttg aacttagtga
     1741 cactatgaag tcaaagatag atgaaatttt aaataaaaga caacacaatg ttgaaggttc
     1801 taagtatgat atagctatgg ttatagttcc tactcttgag gaggaatgtc ctagttaaag
     1861 aattaattat ggcttaatta aagatataag attagagatt gaacatcaaa atttacttaa
     1921 taatcaaaaa atagtttttg gtatggtaaa gtaatgattt ttaatctcta tttttttgtc
     1981 tctttatact attgtgtttg taaatgatag atagtgtgta agatgtattc actatctaag
     2041 aagtgcttag ataatgtata caaagtttgt aagattataa tatttgaaat tgattagaat
     2101 gggtataatt aaaatgtaaa tattaaaatt aaaaattaat ataaattaaa tcaatggtag
     2161 aaatatatta aaagaggaat gaaattagtg gggatggaac atcccttaga gcctataaat
     2221 ttttagtgta aaagtactat aggaatcttc atctttagat gttggctgaa agtagctaag
     2281 gtggagtagt tcaaaattaa aattgagtac aagttgtagt acaaataaaa ggagattaat
     2341 atgattaaat taggaacaca tctttcaata gcaaaaggat ttgctaatgc agctgagaca
     2401 gctgtatata ttggtgcaaa tacatttcaa ttttttagta gaaatccacg aggaggaaat
     2461 gctaaagagt ttaatgaaaa agatataatt aaatttcaag aaattagaaa agcaaataat
     2521 tttgcaccac tgcttgcaca tgcaccatat actatgaact tgggtggcac aaaggatgat
     2581 gtatacgaat ttgcaacaag ggtgatagat gaggatataa gaaggatgga ttcattagga
     2641 attgaataca tgtgctttca tcctggaagt catgtaggtg gtggtgtaga ttttggtata
     2701 gatagaatag taaaaggatt gaacaatgct ataaagggcg atgaaaatat aactatactt
     2761 cttgaaacca tgtcaggaaa gggaactgaa ataggtttta aatttgagca attaaaaagt
     2821 ataatagata aaatcgaaca caaagaaaaa ataggtgtgt gccttgatac atgtcatatt
     2881 ttttcagctg gttatgatat agtgaatgat ttagatgggg ttttggaaga gtttgataag
     2941 gttataggta tagacaggtt gaaaactgtt catctaaatg atagtatgat gccctttgga
     3001 gataaaaaag acagacatgc tcctataggg gaaggaaaga taggattaaa agcattaatt
     3061 gattttatgg aacatcctag tttaaaacat ttaccatttt tcttagaaac tccttttgat
     3121 gatgaaggtc ataagagaga gcttaaaatg ataaaagaaa tattatctag taaataaaat
     3181 tttatttaaa catgtaaaat actccactct taacataatt agggtggaga tacaatcatg
     3241 aaatgtaata atttaaatta atagatagga ataacgaaaa ttgtatacga attaactggg
     3301 tataaagtat gatgatatta tgaatagact gatataaaat ataactatat tatgaatagt
     3361 atgtaaatat attgcaaaga ttctcataat ttgttgtata attaaaggtg acatatttat
     3421 attatataaa aaattattaa aagttaggtg gggaaagata tgagaaattt aggcaatact
     3481 aatatgaaaa taaagagagt tggttttgga ggtattccaa tacaaaggat tactcaagat
     3541 gataccaact tagttataaa tgagttagaa aagcaaggaa taaactttat tgacagtgca
     3601 agagggtata cgataagtga ggaagcaatc ggaattgcca ttgaaggaaa aagagataaa
     3661 tttttcttag ctactaaaag tatgtctaga gattatgatt caatgaagag agatgttgaa
     3721 ataagtttaa ataattttaa aactgatttt atagatttat atcaatttca taatgttaaa
     3781 gaagaggaat atgataatct ttttaaagat aaaatggctt atagtgccct tttagaggca
     3841 aaagagcaag gtaagataaa acatattggt ataactagtc acaatttaaa tactatagaa
     3901 aaagctatag aagatggaaa atttgacact atacaattcc cttacaatat agttgaagga
     3961 caagcagatg atgtgtttaa aaaagctcat gaaaaaggaa ttgggataat agtaatgaag
     4021 cctttagctg gaggtgcttt agataatgca acattggcta ttaaatatat actatctaaa
     4081 gattacatag atgtagttat accaggtatg gagagtgttg aacaggttag acaaaatgtg
     4141 gccgtacttg aaaatcttgt gctagatgaa aaagataata aggaaattga agaaataaga
     4201 aattctttag gtaaaaaatt ctgtagaaga tgtgaatatt gtatgccttg tgctgtagga
     4261 ataaatatac cattgagttt tttatgtgaa ggttattata ctagatatgg attaaaagag
     4321 tgggctaaag aaaaatatga ggttatggat gttaaaccta ctgagtgtat tgactgtggg
     4381 ctttgtgaga gccgatgtcc atatgaactt cctataagag aaatgctgaa gactgttgtc
     4441 gaaaaactag gatagatttt gtttattgaa atcggtgtag taaatttact ccgatttctt
     4501 tttattatat gtggtcttat atacaaatat taaatttaca tagttatagg cattatataa
     4561 atttaatatt tgtttaatac ttattaaata tttgcgtgag tatagatata acacaaatat
     4621 tatttgttgt attagtatag tttcaaatac tagagcatca atataatggc gaataacata
     4681 caaaatcttt atttttcaaa aatattgtag gatatattat atttagatac tggataaagt
     4741 tcaatttcaa ccctagggtt atctttatct ataaaatcaa ataaaagtac tggcttgagt
     4801 tgtttatcat tttttattat tccacttttt tcaataccat cacaaatact ttttggataa
     4861 ttatgtaaat cccccattct cttatttggg aagaataatt tcaagactgt tattagattt
     4921 tcttcaacag tttctccttg atactgttgg tttataaaac ctgcaatcat gttttcgtat
     4981 gcaagatact tagaatattt tccctttgaa ggcatccatg cttgaccatt tatgttgtga
     5041 agtttaaaat tagatttact aataggtcta cctggaattg ttattttaat caaatttttc
     5101 accatcctta tacagtattt attataatct gtatatggtt taaaagcaaa ttgttattta
     5161 aagtgttttt acagagctta tttgtgaact tttccacttt ggatgtagtg atataagaca
     5221 agtgttttat ttacaaattt taaaatctga tatatgctga atatattcat attaggctat
     5281 tataaactta tcacaatatt aatgaacata agtttagtga agagaaaaaa agagaacaat
     5341 ataagtcaag taaagactta ataaacagta atgtaaatgg aatattaaat ataagaaaaa
     5401 atcaatcttc ttacattaat tataggaaga atgtcaaaat ataactaatg atatttgaat
     5461 ttttagaatt tagattaaaa accaaaaagg ttaagtgggt aaaaaagatt gaatagatta
     5521 aaaaggctag gtgagtaaaa acattaaata gctaaaaaag gctaagtaga taaaagagtt
     5581 aagtagttaa aaaattgaag atctaaaaaa gactaagtag gatagaagtg ttaagtaagt
     5641 taaataaaaa agactaagta gataaaaaaa ttaaataagt aaaaaggttg aataagtaaa
     5701 aaatactaag cagataaaaa attaaatggg taaaaaggtt gagtatataa aaaaactaag
     5761 tagataaaaa aattaaatgg gtaaaaaggt cgagtatata aaaaagttaa ataaattaaa
     5821 aggttaaata gattaaaaag gaataatttt atgaataata gggaattaaa agaattttgt
     5881 aatttaatag gattaaattg tgtaggagta gcaggtgtag ataagtatga taatttagaa
     5941 aatatactta aagacaggta taaaaaaggt tatttaactg gtatggaaga accaataatt
     6001 gaaaacagaa ttaatccaag aaatataatg gaggatgcta actctataat agtgtgtgca
     6061 tttccatatt atataaatag acaagaaaaa aataaaaatt caaatttatc aaaatattgt
     6121 catggaaagg attatcatat tgtagtaaag aaatttttac aacaaatttg cgattatatt
     6181 tctaaaaaag taacagattt taaatataag atatttgctg ataatggacc attagttgac
     6241 aggtatttag cttatttatc aggaatagga tattttggaa taaataataa tatcataact
     6301 gatgaatatg gttcttacgt atttataggg tatatagtaa ataattataa gtttgaagaa
     6361 gataaaccac tagaaaaaag ctgttttaaa tgtggtaagt gtgtaaaata ttgtccggga
     6421 aatgctatat tgggaaatta tgatatgaat cctaagaaat gtttatcata tataactcaa
     6481 aaaaaaggag atttgtcaca ggaagaaaaa gctgttttaa agagtggcaa aaaggtattt
     6541 ggatgcgata tttgtcaaga agtatgtcca cataatgttg aaataccaac aactcatatt
     6601 ttggaattta aagaaaatat aatagattat ttggattatg atgagataca aaatatttca
     6661 aataaagaat ttaaaaggag atatggagat agggctttta gctggagagg gaaaaatata
     6721 ataaagagaa atatggaaat aattttagat aagtataatg actaataata agagtctgtt
     6781 tcatatactt taatgtgtat ttactttaac acttgttgtg tgaaagtcat attatatcat
     6841 taaagttaaa tatggggagg tgggggaata ttgaaatata ataatacatc tgaaataaat
     6901 ccactagatt gtatattggg tcaagaaaga gctgtagaag ctatggagct aggtttaaaa
     6961 ataaataatc cagcatacaa catatatata gctggtgaat ctggaactgg taagagtact
     7021 tatgctctaa aggttttaaa tgaatatgca tccaaaaaaa atacgcataa agactggtgc
     7081 tatatatata attttgaaaa ccctagagag cctattatag ttgagttaga aaaggggttt
     7141 ggaagagaac ttaagaaaga tatggagaaa ttaattgaga gtcttttgga agattttaag
     7201 aatgcttttg aaagtgaaaa ttttgaaata gaaaaaaata aacttttgga tgaatatgaa
     7261 atagaaaaag atatgctttt gaagcaaatt aagaaatatg gagaagaaaa aggatttaaa
     7321 ttaaaacaga gtaaaatggg aatagttttt atccctttga aagaagagga agatgaaagt
     7381 gatgagtctg atgaagagtt ttacaaagct aagagagaac tagaaaatat ggcgatacag
     7441 gtcgtatata aaattagaaa cttagaagaa atagctgaaa aagcagtgtt agagcttgaa
     7501 gaagagatag ctaagcttgt agtagaacct catatcaaga gattgtgtga aaaatatgaa
     7561 aattatgata agattcaaga ttatctggaa aatataaaga aagatataat agaatatatg
     7621 tatttgttct atttagatga agaagagtta aaagataagt atgataaaga gcattttatt
     7681 aaatataaaa ttaacttatt tgtagacaac ggaagttcaa aaaataaaga atcagcacct
     7741 gttgtagtag agataaaccc aagtccagca aatctatttg gaaaggcaga gtatgattat
     7801 gcaaatggaa atataaagac tgatttcaca aaactattat caggagcatt tcatagggct
     7861 aatggtggtt atttagtact ttatgcagac caattactaa aatatacaat gtcttgggaa
     7921 attcttaaaa agactttgca gactaaaaaa gtaatattgg aaacacaaac agcaattaaa
     7981 ccagaaaata tgccattaga tgttaagtta gtactgattg gaagtcacta tatatatgat
     8041 attttatata gatatgatga agattttgaa aaatatttta aagtatttgt agattttgat
     8101 agtgaaatgg ataaaaatga aggtaatgaa gagggaatag caagatttat agcatcccaa
     8161 tgtgataaga ataacttaag acattttact aaatcggcag ttgaagaggt tgttaaattt
     8221 agcactagat tgtccggtga tttagaaaaa ctatcaacta agtttaataa aataatggaa
     8281 gttgtaatcg aaggaagtgc ttatgcagaa tttagaaatt cagagtatac agagcagtgt
     8341 gatgttaaaa aagcaataag tgaaaagaga aaaagaatca atagagtgga aacacatatg
     8401 gatgaatcaa ttgaaaatgg atttactcta atagaaacag agggaagaaa agtaggagtg
     8461 ataaatggtc tgtcagtctt aagtacagga gaatactctt ttggtagagc ttcaaggatt
     8521 acagctacca ctagcccagg aagtaaaggt atagtaaata tagaaagaga agtaaatatg
     8581 agtggctcta tacataacaa aggagtgctt atattaggcg gctatctttc tgagaatttt
     8641 gcacaggatt tgctattgtc actaaatgca tatgtttgtt ttgaacaaaa ttatggtgga
     8701 atagatggag atagtgcatc ttctgcagaa ttatatgtac tattatcatc tcttagtgga
     8761 gtaccaatca aacaaaatat tgctgttaca ggttctataa atcaaaaggg tgatatacaa
     8821 gtagttggtg gtataagtga aaagatagaa ggattttatt ctatatgtaa aaccaaaggg
     8881 ttaaatggtg agcaaggtgt cataattcct agaaaaaatc aaagaaatct cgttttatca
     8941 gatgaagtta atgatgcagt gcaagatggg aaatttaaga tatatactgt tgaaagagta
     9001 gaagaggcta tagagatatt gacagatgtt aaatttgaag aaataaggaa actggtaaaa
     9061 gaaaaattaa tatctttcag taagatacaa actgtgtcaa aagaataagt ttattataga
     9121 atttaatggg tatttatgta taataaaaag caagaacttt aataagtttt tgcttttttt
     9181 gtgaaatcta tataaattaa attcttattt tgtatgtttt atatattgat gtttattttt
     9241 tgttgatatg attaaactat gtataaatag cgttttataa attagtattt taaaggggga
     9301 taattatgaa aaatgtaaat ctaaaagaga taaataaaaa agaggtaatt gataaagaag
     9361 agataaataa aaaaggtata gataagaaag acgtaactga gaaagaaaag ataaataaaa
     9421 aaagtgaaca cgacaaaaaa acaaagaata gtagttctag taaggatacg caaaaactaa
     9481 gtaaaggaat taaaagtaag tctaaaatta aaaatgagtc agcaaatgaa aaagatgtaa
     9541 ataagatttt agatgagtta gaaaagttgt acccagatgc taaatgtgag ttaaattatg
     9601 ggacggcctt tgaacttttg atagcaacta ttttatcagc acaatgtact gatgtaagag
     9661 tgaataaagt tacttcagag ttgttcaaaa agtacaatac agctaaagac tttgctaatt
     9721 tgagtattga agaaattagt aaggaaataa agagttgtgg tctttacaaa agcaaatctc
     9781 aaaaaataaa ggatacatct gaacagctat gtgaattata tgatggtgaa gtgcctgata
     9841 gcttagaaaa gcttataaaa ttacctggtg ttggaagaaa aacggcaggg gttgtactta
     9901 gcaacgcttt taatcatcca gcaatagcag tagatactca tgtatttaga gttagcaata
     9961 gaataggaat agtggatgag ccaaatcctc aaaaaacaga atttgcactc atggaagcta
    10021 taccaaaaga aagatggtca cactcacatc atgtattaat ttttcatgga agaagaatgt
    10081 gtaaagctag aaatccagaa tgtgcaagtt gcccaataaa agaagattgt aattattata
    10141 aagaacttaa tgaaacgaaa taacttttag aaagtaagtt gtacaagagt cattttctga
    10201 tataataata cttgtgaaaa tgttagataa aaaactatta tatacatgaa ataaaaatta
    10261 ttattaacat tattttatat aatagtataa tattattgtt attctattta taagataaaa
    10321 tttgataaat agaagcaata tttagggagg attaaaatgt cattaaatat agtgttagta
    10381 gaaccagaaa tacctcaaaa tacaggaaat ataataagaa cctgtgcagc aacaggttca
    10441 actttacatt tagtaaagcc actgggtttt tcattggatg ataaacacct aaaaagatgt
    10501 gggcttgact attgggatat agctgatata caatattacg atagctttga agaattacaa
    10561 gaaaagtatc caaacagtaa atactttttt tctacaacaa aggctaaaca gtcacattca
    10621 gatgtgaaat atgaagaaaa ttgttttata gtatttggga aagaaacaaa gggattacca
    10681 gagactttgt tgaaagcaaa tatggatact tgtattagag tgcctatgtt agatgttgaa
    10741 aaagctaggt cattaaattt atcaaattca gttgctattg tagtgtacga ggctttaaga
    10801 caaataggat acccaaatat gaggtagttt aacaagccta aatgagaaag gagcatactt
    10861 aatgaaaaat ctaaagataa tctgtgacag tttatcggac gtgccaaaag atttgttaga
    10921 taaatatgat atagatatag tttcgttaac agttatactg gaggaaaaag aatacaagga
    10981 tagaattgat atatctggag atgaatttta taaaaggctt agagaagaaa atgtatatcc
    11041 aaaaacttct caagctactt atgcccaatt taaggaaatt tttgaaaaat atacaaatga
    11101 gggtagagag atactttata tagcagggtc tgctacagct acagggacgt atcaaagtgc
    11161 tgtaatggca aaaaatgatg tagagggtaa aatatataca tatgactcta acttgctttg
    11221 tatgggaact ggtttacttg taataaaagc aggagaactt gcaagtgaag gaaagactgt
    11281 tgatgaaata ttgcctgttt tggatgaatt aaaagagaag gcttttgtta tattttcagt
    11341 agatacactg gaatacttac aaaagggagg tagaatatca tctactaagg cagcaatagg
    11401 aagtatatta aatataaaac cgattcttga aataagagat ggactagttt cgcaaatatc
    11461 tcaagtaaga gggaaaaaga atgtaatttc taagatgatg gaattgataa aatcaaattg
    11521 tggggatgat ttatcagacc aaattgtgta tataggatat agtgatgatt ttaaggaaaa
    11581 agaacaatta tctaaggtta tggaagatga attaagacct aaagaaatta gatttttcca
    11641 aattggggtg tgtataggtg cacatgctgg tcctggtgtc actggtatca tatgtttaaa
    11701 aaataaataa atatattttg aggtaatatt atttattttt ttattaaatt aggatagaat
    11761 actaaatatt gagatgctaa attggattta ttttggataa aattaagtgt tgatttatta
    11821 gatttagttt gtagggaagt gtgtaaatag ataagagtct attgcacact tttttatagt
    11881 aatatttatt aaaaaatttt caaaatgtac ttgtaaattg tatataaaaa aattataaat
    11941 aggatgtaaa agtaaataag agtaaaaata tcataaatgt atgaggagtg aaatttaatg
    12001 cgtttcaaaa gaatattgat atttatatta actgtagcaa tgacagctgg aatgttggtt
    12061 ggatgtgata gtaatcaaag taataatcct agttctagca aagataataa aagtgatata
    12121 agcgacaaaa gtaattcaaa aaaatctgta tctatgatac tagatattga aggaacaaat
    12181 aatgaagcta tgaataattc agctttatta gcattaaaca atgctcaaaa gaaacttaat
    12241 atagacacaa acaaagtgga atcagatgat tcatcaactt tttctaatag tatagatatt
    12301 ttatgtaatg acaattacga tttaattata gctgtaggag ctagatttgc aaaacctttg
    12361 gagatggtag ccaagaaata tccaaagcaa caatttgcaa ttatagacta tgagtatgac
    12421 aagcaacctt ctaatataac ttcaatttct tatgaagata ataaatctgg atacctagct
    12481 ggattaattg ctggaaagat gactgaaagc gataaggttg gttttatagg tggaacaaag
    12541 agttcgagta gagacaaatt tgaaacaggt tttaaagaag gtgttaagtt ttctaatagt
    12601 agcataaaag atatttcagt tgaatatgca gatgttttta aagatagaaa atcagtagaa
    12661 tctatagcta aaaagatgat ggataatgga gtggatatta ttttttctac tactgaagat
    12721 gattcaaaag ctgtaatagg tgcagtaaga acaaaaaata aaaaagttat agcaacaaat
    12781 aaagaccaac atgagttagc tccagaaaat gttatcagtt ctattgtaaa agattttgag
    12841 aattctactt ataatttgat acaaagcttt gtaaaaggaa attataaagg tggaaaagtt
    12901 atagaaaata cagttaagtc tggaagtact gggcttgccc aaaatagttc tcaaaatatt
    12961 cctccagatg tgttagaata tgttagtaag aacaataaat aatcttctat aatatccagg
    13021 aatacttatg actttagtta tgagttgaac tggctgttat aatagacata tatagaaagg
    13081 catatgtaga ggtaggtaat gcctatctaa atcagggagt gaattttgtg aaaataaaaa
    13141 ggatattgat ggtttgcttt gtggtagttt ttacaatttt tatattagct atatgtaatt
    13201 ttgattttgg gtttgataaa gtaaataaga ataattctaa tatagatagt gctaaatcaa
    13261 aacagagtga gtctaaaaat gtaccgactt tagcagaaaa ggataaatca aatagtaaaa
    13321 gtaagaataa agaagatgag acagaagatg atttatatgt aagaaaaact gttggagtat
    13381 tattagatac tgaaaactta aataatgaat catataataa taatgtacta tcagccttaa
    13441 aaagggctga cgaagatttt gattaaaaaa tagctacgca cactcgtgac tttagtcatg
    13501 agttagtagc taaaatagtc agcatatagg gaaacttata tgtagagata ggatagaacc
    13561 tatccaatac tacttgaatt gctg
//
LOCUS       DAMJVM010000092        13569 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.096, whole genome shotgun sequence.
ACCESSION   DAMJVM010000092 DAMJVM010000000
VERSION     DAMJVM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13569
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.096"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            240..1178
                     /locus_tag="PC523_002815"
     CDS             240..1178
                     /locus_tag="PC523_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2476321.1"
                     /translation="MFRKVLYSLTLLSISIIFLVGCSNSQNKQNANENKEIQSQEDTK
                     KIDSSKDTSNVIVSDGIDKPSKNATNNDNNKLDVSSLDNTALDWFYIPNNKHKTPEVN
                     TDIGFKFSDYDAIYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTY
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKN
                     MVKFFRPPMGKYSEKSLAMTKNLRYKTVFWSFAYRDWDTKKQPPHEEATQKIMANLHD
                     GSILLLHAVSKTSTEILNDFITNARKLGYEFELLEY"
     gene            complement(1535..2908)
                     /locus_tag="PC523_002816"
     CDS             complement(1535..2908)
                     /locus_tag="PC523_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476322.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISVAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYMFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFIPVITGLIVTT
                     IVAMFII"
     gene            complement(3074..4396)
                     /locus_tag="PC523_002817"
     CDS             complement(3074..4396)
                     /locus_tag="PC523_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2476323.1"
                     /translation="MISKIKPSKLGVILLMYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNRLMDDFQTYVNNNGIRADDGDDIAKWNSDNWFVSIKIYKGNRVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIIVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSN
                     TLEKQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            complement(4389..5093)
                     /locus_tag="PC523_002818"
     CDS             complement(4389..5093)
                     /locus_tag="PC523_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2476324.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LADSTIDLIILDVVMPVKTGFVACSEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEKEPNILKTKELCVNLDTQDVTLNGQPVF
                     LTTIEYSILVLMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            complement(5276..6340)
                     /locus_tag="PC523_002819"
     CDS             complement(5276..6340)
                     /locus_tag="PC523_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2476325.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFIMMFIYVACEGINIYLGMKALRQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGVMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVVGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVITSQI
                     EEYVKGAQYIKQNPKLVVQILVITIIQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     IVHVKSSEKVKSVKKVAYVR"
     gene            complement(6343..7041)
                     /locus_tag="PC523_002820"
     CDS             complement(6343..7041)
                     /locus_tag="PC523_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2476326.1"
                     /translation="MYVVGLIIIVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIIGLHSLEH
                     KNAWLYSYSYVKKDFIESTNIMKNLGVDVSYYRPPWGHTNIFSNLFVKKYNLKMTLWD
                     VMAEDWNKNSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKVAIPK
                     LKASGLKFVTPERM"
     gene            complement(7032..8129)
                     /locus_tag="PC523_002821"
     CDS             complement(7032..8129)
                     /locus_tag="PC523_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDF2476327.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEITIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDELLNEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHINHSTKKNILIMGGGLGLLPKSEQFYRELNDL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKGADLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDVTLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGECRECM"
     gene            complement(8454..9698)
                     /locus_tag="PC523_002822"
     CDS             complement(8454..9698)
                     /locus_tag="PC523_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDF2476328.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            10026..10814
                     /locus_tag="PC523_002823"
     CDS             10026..10814
                     /locus_tag="PC523_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2476329.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            10814..11287
                     /locus_tag="PC523_002824"
     CDS             10814..11287
                     /locus_tag="PC523_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2476330.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGITGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(11379..11819)
                     /locus_tag="PC523_002825"
     CDS             complement(11379..11819)
                     /locus_tag="PC523_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HDF2476331.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGLKTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(11834..12127)
                     /locus_tag="PC523_002826"
     CDS             complement(11834..12127)
                     /locus_tag="PC523_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDF2476332.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            complement(12170..12571)
                     /locus_tag="PC523_002827"
     CDS             complement(12170..12571)
                     /locus_tag="PC523_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476333.1"
                     /translation="MKKCLYCNRDIKSPNNNLSIKYDDNTNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILVFIVIITVLYFKQVGK"
     gene            12804..13448
                     /locus_tag="PC523_002828"
                     /pseudo
     CDS             12804..13448
                     /locus_tag="PC523_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT         4676 a         2229 c         1607 g         5057 t
ORIGIN      
        1 actcattcaa tgtctttgtg cttgttattt tgttacttct tgttgagcac atttattata
       61 ataacacctt ctaacaatat ggtcaactac ttttttcatt ttttttaaac tttattgatt
      121 attcacccat tgtcatacta ttgtgacttt atttttaaga atgtaactat tattaataat
      181 agtttacaat tataataata ctatatgtag ttgttattaa ttcagaaagg agcttcaaga
      241 tgtttagaaa ggtattatat tctttaacac tactttctat atctattatt ttccttgttg
      301 gatgttcaaa ttcacaaaat aaacaaaatg caaatgaaaa taaggaaata cagtcacaag
      361 aagatacgaa aaaaatagat agtagtaagg atacttccaa tgtaatagtt tcagatggaa
      421 tagataaacc atcaaaaaat gctactaata atgataataa caaattagat gtatcctctt
      481 tagataacac tgctcttgat tggttttata taccaaacaa taagcataag acaccagaag
      541 taaatacaga tattggattt aaattttctg actatgatgc tatatacaat gggcctacaa
      601 aagatggtca gaaaacacta tatcttacat ttgatgaagg ctatgaaaat ggatacacca
      661 caaagatact cgacaccttg aaacaaaatc aggttaaagc agtttttttc gttacatatc
      721 catatataaa ggaaaacaaa gatttagtaa aaagaatggt ttctgagggg catatagttg
      781 gaaatcacag taaaactcat ccatctatgc caacaaaaac aagcaattta aaaaacttta
      841 atgacgaatt atatgatgtt gaaaagttat ataaagatgt aactggaaaa aatatggtta
      901 aattttttag acctcctatg ggaaaatatt cagaaaaaag tttagcaatg actaaaaatt
      961 taagatacaa gactgtattt tggagttttg catatagaga ctgggatact aaaaaacaac
     1021 cacctcatga agaggcaaca caaaaaataa tggctaatct acatgatggt tctatcttgt
     1081 tactacatgc agtttctaaa actagtacag aaattttaaa tgattttata acaaatgcaa
     1141 gaaaactcgg atatgaattt gaactacttg agtattaatg tttagaatta catctaaaat
     1201 ctacaaacta gtatagttaa tatagtatat gtgataaaat aataatttaa aattagaatt
     1261 tttgtttggt aatagtatag gtaaaaaact tgtgatgtct tacatatgtt ggtagtacaa
     1321 gcaataaaaa ctcaataaat ttggcaagat actaaaatag ataatagtat tttagatgtt
     1381 atattagtat aacaaaaaat attattctat caaagaatac tattaaaaat atcaataaaa
     1441 atagatgtat ggcttagaat tagtaaaact gttatgataa attatagcta cacatctatt
     1501 tttagtttgt ataattaaag ttttatttaa ttcattaaat aataaacatt gctactatag
     1561 tggttacaat caatccagtt ataactggta taaaatttcg ctttgccaat tctattggac
     1621 taactccaca aattgccgct gctgaaataa gcccccaagg aactaaacag cctccaccta
     1681 cccatatacc actgatttgt cctaatgcag ccaatgcacc aacacttgca tttatagcag
     1741 ttccaaacac agatgcaagt gaaccaacta atgaaattcc agaaaatcca gaaccatcaa
     1801 gtccagttat tgctcccact acagtttcta tacctgatac agcaaattta ttaagtggta
     1861 ttgtctgaga aagagctatt cctatatctg ataataaacc ttgtgaacct ggtgctaata
     1921 cctttccaaa tacacctgtc aaaggtgcaa cttcacccat atagaaaaaa gctgctattg
     1981 gtataattgc gccaaatatt tttatcccaa acacaaaacc atcaattata ttctcacata
     2041 cattttcaag actatctttg ccaaaattta taacatttat aattattatt aaaaatactg
     2101 ctgttccacc taaaagagct gatgcatctc ctccttttag gtcaaacata tacattgcaa
     2161 ttatatcagc aagaaatcct aaagctacta aaatagttgc tattttagat tttccattaa
     2221 actcttctac ttcttcactt tcaaaacctt ttagttcttc tctaaacatt cctttgttaa
     2281 tgtctctttt caatgtataa aatgccacag atatggttac gattcccatc acccaaaaca
     2341 aaatcatacc atcatttata acatatgata ctgcaactcc tgctgcacca gctgttatac
     2401 ttggagctcc ttgaatgaca aagtctgtag ataaggcaag accatgacca aacaaattta
     2461 atgctactgc aacaccaatt gctggtagtc cagctctcat agcaacaggt agaaatacag
     2521 ctccaactaa ggcaactgct ggagttggcc agaaaaacca agaaagaact aacataacaa
     2581 atccagttac aaaaaatgct gtattagaat tttttataac ccttgaaaac ggtcttacca
     2641 tatactgtat agcattattg gcttctaagg ctttagataa tgcaaccata atagctataa
     2701 ttaagataat tggtcccaac tcatttaaag atataataaa agagttaaac actgctgtta
     2761 tagcaccaaa aatatttttt tcaaagaaca gtcccataaa aaaaacacct aatatacatg
     2821 gtactattgt atcctttttc ataatcatag taattaatat aattaccata aatagaatgt
     2881 aaatataatg tgctcctgtc aaaaccatat tattatgtcc ccttttttta agataataat
     2941 attatatggt tttgtaagta aacttgttac ttgcctagag ttacacaata tcgaaccttc
     3001 cataactaaa gtcacagagt tcctgcttca tagaattttg cataatcaat tacacaatat
     3061 ataatttaaa tttttatgat tcttttgctg gaatagatat ttcaatagca aattctttat
     3121 catctgagtt tacatttata cttccattat gatatgcaac tattttctca caattcttta
     3181 taccaattcc aaaactttct acattcttag aaaaattgct tactctattt ttttgtataa
     3241 ttaataattt agaatcctct actttaatta aaaatactat aggtttatct ttcctagcat
     3301 attttattat attagaacat aaattatcaa atatccttct aaattgtgta acatctacat
     3361 aaatatgata ttggttataa agttcatttt ctattgttat atgaaatcct ttctcctcca
     3421 atgtatatga actatctaaa agtaattgaa aaataaattc attagcattt attttttcat
     3481 acccttttga aacttcctct ccagaattaa caaaatgggt aaaaaggtca tctgttaaat
     3541 cttttatttg atatgcttta gaccttacat tttttatata ctgttccaat tgctcaggac
     3601 tattatattt atggtcttta attaaatcta aataacaaat tactgatgtt aatggagttc
     3661 taacatcatg tgatatcgaa gttactatat ctttattttt attcttcatc tctcgttctt
     3721 ttataagttg cttctccaat gtattgctca tctcattaat actagatgca agtgatgata
     3781 attcatcatt accttttacc gggattctat attttaaact gccactctcc actattttta
     3841 tacccatttc tattttcctt aggtacttta tctttctact aaacaatagt aagaatgtta
     3901 taataaatgt aagtgccata aatgtaatag aaattgcttc tataaaattt tgaaacctaa
     3961 atctaaataa agctactaca attatattag ctttaatatc agaaaattgt atttcataat
     4021 ttctagccat tggactgcta taaaactcat tttcctcact cttatcaggt acttcattag
     4081 gatagtataa tgtatcataa aaaaccctat ttcctttata tatcttaata gaaacaaacc
     4141 aattatcact attccatttt gctatatcat ctccatcatc cgctctgata ccattgttat
     4201 tcacataagt ttgaaaatca tccataagtc gatttacttc tcttttaaaa tatactttcc
     4261 cttctgatac atcttttact atagattcaa cagcagagta aaatggtata tttaaaatga
     4321 aagccactgc agctgagatt actacataca tcaatagtat aactcccaac ttagatggct
     4381 taattttgct aatcaatata ataccccttt ccccatgcgg tttttatata ttgcggattt
     4441 ttttggtctt tttctaactt ttttcttaaa tttctaatat gaaccattat tgtattattt
     4501 gctgtataaa aatactgctc atcccaaata gtttcatata aattttcaga tgagaaaact
     4561 ttttttctat ttttcaacat aagaactaat atactatatt ctatagttgt caaaaacaca
     4621 ggttgtccat ttagtgtaac atcttgagta tctaaattga cacataactc ttttgttttt
     4681 aatatatttg gctctttttc cttttcctta ccctggtata tataataacg tctaagtaac
     4741 gacttaaccc ttgaaattaa ttcagaatat gaaaaaggct ttgataaata gtcatctcct
     4801 cctgctgaaa atccaataac tttatcagaa tcttgagttt ttgcagttaa aaataatatt
     4861 ggtgctgtcg ttttttctct aatttcacta catgcaacaa atcctgtttt tacaggcatt
     4921 acaacatcca aaataatcaa atcaatcgta ctatctgcta agtttacagc ttcttctcca
     4981 ttcgttgcca taataacttc ataaccttca ccagttaaaa gtatttctac gatttctcta
     5041 atttcaggat tatcatcagc tactaaaatt ttttttggaa tttctttatt catcaccaag
     5101 ctcctttttt tgctatattt cttaaatatt tcaaactata taaaatatta tactttatta
     5161 gcaaaagctt agcaatgtca cacgttttaa taccttctac tttggtaaac atgtttaaaa
     5221 cttaattaaa atgtatacac aaattgacat attaataaaa ataatctata taatattatc
     5281 ttacataagc aacttttttt actgatttta ctttttcaga agattttaca tgtactatta
     5341 tacatattaa tccacttata gctacaaatg cgtaaaaact tattcctcta ctaagtaaca
     5401 ttgccggaac taataagtga ccagggaaaa atatcttaaa taatgtcata aaactatttt
     5461 cagaagctcc aacagcccct ggcaatggta atgatgaaac agcaatatta agcaaggatt
     5521 gtatcgctaa tatctcaaac acagtatatg ttgatagatg aaaagctttg tatacaaaga
     5581 aaggaactaa aaacatagct gttatttgta taatagttat gactaaaatt tggactacaa
     5641 gctttggatt ctgctttata tattgcgcac ctttaacata ctcttcaatt tgagatgtta
     5701 taacttttct tgaactttca acatctttta ttattcttaa cttacctaat aagtttgtta
     5761 ttgatattat aaaattattt acaagttttt ttgaaaatat aattgcaatt actccaacaa
     5821 caagagcaac atttgttatt acaccatata ttagtaaaat gttcataccg cttacattat
     5881 ttaatataaa ctcacgttcc ataataaaca ttactccact atatgccaat atgactactt
     5941 gatgaataac aacaatgact aatagtataa gtgaagaata tgatatattt atatcatctt
     6001 ttttcatata ataaacttgt gctggttgac cacctgatgc tgaaggcgta atagaactaa
     6061 aataaaatcc aataaaagca tatcccatac attttaaaag agttgttttt tgtcttaaag
     6121 ctttcatacc taaatatata tttatacctt cacaagcaac atatataaac atcattataa
     6181 atccaattag tataaacata gggcttgcac cttttaatga ttttatcaat gattcaactg
     6241 actgccctct aaaaacaaaa tatcctgtta tgcccattag taaaactaaa aaagctacac
     6301 tacctaggca tttatttttc ttttcagttt tacttatcat aactacatcc tttctggtgt
     6361 tacgaacttt aaaccactgg ctttaagttt tggaatagct acctttaagg cttcaatagt
     6421 acgctcaggt gcacctactg caccacctga attttcacct gcatcatgta aacaaattat
     6481 agaattttct tttgttctac tcattaattt gtttataatg atgtctactg tactattttt
     6541 gttccaatct tctgccatta catcccataa ggtcattttt agattatatt ttttaacaaa
     6601 caaatttgaa aatatattag tatgacccca tggtggacga tagtagctta catctacacc
     6661 taaatttttc attatattcg tactttcaat aaaatctttt ttaacataac tataactata
     6721 aagccatgcg tttttgtgtt ccaatgaatg taaaccaatt atatgatttt ctctcaacat
     6781 tctttttata atttcaggct ctttttcagc attctttgct actacaaaaa acatagcttg
     6841 tatatcattt tcttttaata cgtctagtaa tttttctgta tatctcttat ctggaccatc
     6901 atcaaatgta agtgcaattt cattttctcc agccatattt tttaatacct ctttatttaa
     6961 tagtttattg tagtatgtag gtataattga atggactaaa aaaatcaatg caacaatgat
     7021 aattaaacct actacataca ttccctacac tctccttgtt catcaagtga aaataaaata
     7081 ttttctaagg tattattatc aaactgtttt tttaattctt tcatgttact actcatttct
     7141 ttaagagtaa catcatcata aattaaatct tttatctcat ctacatagta ttttttattt
     7201 ttaccaagta ttctcccaat ttcattattt aatataaaac tagcattatc tatctcttgt
     7261 tgtaaaaatg gattaaatgc caatattgga agttctgaat aaattgtttc gaatagtgtt
     7321 attccacctg gtttagaaat aataaggtca gcacctttca tatatttata tacttcattg
     7381 gtgtaaccta ctacctctat attttcatat ctaccatata gtttatagta cattttttta
     7441 ttattaccag ttattacagt agtctttact ccttctaaat catttaactc tctgtagaat
     7501 tgttctgatt taggtagtaa tcctaatcct ccacccatta taagaatatt ttttttagtt
     7561 gagtgattta tatgttctat tcttttaaat tcttttttta ctggaatacc attaacttta
     7621 atcttagatt catctattcc tttaaaaaca agttcttcct tagtggattt actagctact
     7681 aaataacaat ctgtattttt actaatccat tctgagtgac tacttatatc tgttatgcat
     7741 gtaattaaag gaaggtttga gttatacttt tccttatatc ttgatactaa ttgagaacaa
     7801 aatggaaaag ttgaaataac tactgttggt tgtacttcat taagcaattc atctaattta
     7861 tttaaaaaat aatctgaaaa agtaaatttt atatctttct ttccattttc agcacactta
     7921 taaaacaaat tataaagact acttccccga tttacaagaa tagaaaaagt cttgtacatt
     7981 ttatctgaat agttaggcat aatatattca aatatatctt taattgttat ttctgaatta
     8041 tcaaattttg cttttatatc ttcacttaaa gaatttgatg ctgaataatg acccatacca
     8101 aattttcctg taagaattaa taccttcata agattccctc ctaattaaag cttttctttt
     8161 catataatta ctatacttat aagtatagac tttaaatctt tagaaaaacc tagtataatt
     8221 cttaagaaat cttaaagttt taattaaaaa atacaaagag accatctaaa aatagagata
     8281 accgtatttt ttattattta aataacacaa atatgttcga ttccatacaa aaaagagtgt
     8341 ctcatgaact aaaaagttca ttttgagaca ccttttttat atctacatgt attcaaatac
     8401 atacacaatc attgtaatta tttaaatgta ttttatttag ttgttcttta taattaatcc
     8461 ataatattat attcttcata taaaggatat ttttcagtta aaccaacaac caatgttcta
     8521 gccttagatt cttcatcata agttaaacat aggtctatag cttcagctat tattgccata
     8581 tcttcttctt tcatgcctct agtagttaca gctggagtcc caagtcttat tccactcgta
     8641 acaagagcac catttggatc aaatggaatt gtattcttat tagctgttat gtaagcatca
     8701 tccaatcttt tttctgctgc tttaccagtt atatttttat tagttaaatc taatagtatt
     8761 aaatgattat ctgtacctcc agaaatcaat ttaaaatctc ttttaataag ttcttcagct
     8821 aaggcttttg catttttagc aacttgtact tgatattctt taaactcatc acttaaagct
     8881 tctttaaagc aaactgcctt agaagcaatt atatgttcaa gaggtccgcc ttgaatacca
     8941 gggaatattg ctttgtctat atctttagca tatttttctt tgcaaagaat aacaccccca
     9001 cgaggtcctc ttaaagtttt gtgagttgtt gttgtaacaa aatcagccac ttcacatgga
     9061 ttctggtgaa gacccgcagc aacaaggcct gctatatgag ccatatctac cataagcaat
     9121 gctccaactt catcagctat ctctctaaac tttttaaaat ctatttctct aggatatgca
     9181 cttgctcctg caactatcat tttaggtttt acttcatgtg ctatctttct aacttcatca
     9241 aaatctataa gtccactgtc tttatctata ccatattcat aaaatttata atactgacca
     9301 gaaatattaa ctggagcacc atgagttaaa tgtccacctt gagataaatt cattcccata
     9361 acaacatcac ctggttttaa catagcaaaa tatacaccaa tatttgcatt tgctccagag
     9421 tgaggttgaa cattagcatg ctctgctcca aatatttttt tgattctatc tattgcaaga
     9481 gtttcaactt catctatata ttcacaccca ccataatatc ttttgcctga gtaaccttct
     9541 gcatacttat ttgtaagatg acttcccata gtttccatta ctggaactga tactatattt
     9601 tcagatgcta ttaattcaat atttctttgt tgtcttttta actctctttt catactttca
     9661 taaatctcag gatccgcttt tttcaagttc tcaaacatat tactcctcct agttattatt
     9721 taaattttat ttttacagac atttcataat tttcgttttt atcaatatga taatttatta
     9781 ttatagtcct ttagcaatta atagtcaagt gttttgccat ttttccatat ttaacataat
     9841 tactttcttt atttttaagt taaaatataa taaataatat tgtctaattc ttttaattgg
     9901 atataattta aatgtgacta ttttgaaaca aaaaaaagat aatatattta tatagatatt
     9961 aaggaatatt acttaaaaat ttgatataat ttcaatatta cgttttttaa tttggaggtg
    10021 tatttatgaa tcaaaatgag gaacttgaat ataagaaaaa tattctaaaa ctagctctat
    10081 ttgtagggga acttatgata aaaaatggag ctgaaacatc tagggttgaa gacagtgtcc
    10141 ttagaatatg caggtcaaga gggttttatc atgtaaatat ttttacaact cctacagtaa
    10201 taattatttc agatgagaaa tttgatggct tttcgtttat gaaaacgatt caatcaagag
    10261 gtataaactt aaataagatt tctttattaa atagtttttc tcgagaattt gttagtgaca
    10321 aagagtatga tattgataat gctattgcac gtctttatga aatacaagat gtaaaacctt
    10381 atcctttatg gttattttac tcttgtacag gaattgcatc tgcttgtttt gcttgtttgc
    10441 ttggagggaa ttatgtatta aattttatac ttacgtttat aatagcaatc tttgctgcaa
    10501 ttacttatga taaaacgatg aagataagtg caatatctgc gttttcctgt ttagtatctt
    10561 cttttttaat tgcactttca ggagttctgc tagttgaaat aggcatttta gatgacccta
    10621 aaatgcttat agtcggcgcc ataatgccac tactcccagg agttgctttt ataaaagcta
    10681 ttagagattt aatatcaggt aatttaatat caggaatttc tcgtgctttt gacgctgcta
    10741 tgattattat tgcaattgct tctggagttg gttttgttct tgatacatgg tatagaatag
    10801 gaggtccttt ctaatgactg atatgtcaat gattttacat tttttatttt caggcgttgc
    10861 aactgctgga tttgctgttt tctttaatgc tccactctat ctacttcttc cagctggaat
    10921 aactggagga attggttgga ttgtatatgt ttatttattt aattttacta caaatgccgt
    10981 ttttgcaggt tttatagctg ctgctttggt ctcagcatgc agtgagactt tggcaagaaa
    11041 actaaagcaa ccagctattg tgttcgtaat tccagggatt cttcctctaa taccagggat
    11101 tgggctatat aatacaatgt tgtctttgat tcagaagaat tattctttgg ctatgtcaaa
    11161 aggtacagat gcgctatttc taagtgctgc aatagcactt ggtgtattag ttgtaacttc
    11221 ttttgtaagg acattgaatc ttttgaaaat cagaaaaaac tttattcctt acagaaaatc
    11281 aaaataaaaa tataaaaata gcaatgtact taaactttat aaatacacat tgagtattta
    11341 tttaaaaagc acattgctat tttttaatta taattatctt aaaggtcaat taatattcgt
    11401 gttattactg gtataaactc tctactgtct ctgaattttt tgtcattctt ctttaagtat
    11461 aaataacaaa ttattgtaag acaaattcca actagtccac ttatagcttc aactccagtt
    11521 ttcagtccaa ttgcccccaa tatataataa gtccctattg ttcctacaag aagtgctata
    11581 agaggtatta tataagccat tacagctgct tttaatacat tcatattatc catactcact
    11641 tctacatggt caccaacttt tgcaccaatt gtgttgtcta cttctactaa tatttctttt
    11701 gactctgctg aatttgtagt agcacatttt ccacaagcag cacaagctga atgtctttgc
    11761 atttttaact ttgcagttct atcatcaaca atttccataa taaatccttg ttgattcata
    11821 actaaccgcc tccttatttt gtaaatttat ttatagtatc cataagctct tttatcatct
    11881 cttcagtttt atcagcatca tcatgtttga tactttctgc tacacatcct tttatatgat
    11941 tctctaagat aattgtgcct actctattta tagcagacct tatagcagat atttgaacca
    12001 atatatcaac acaataacgt tcttgttcga ccattttttg tatcccctta acttgacctt
    12061 ctattctttt taatcttttt atgagagctt ccctctcatt gtttggtgct aattttctct
    12121 cttccataaa ttcacctcat actctaatga tgtcttatgc aattatgctc tacttaccaa
    12181 cttgtttaaa atataataca gttattatta ctataaatac caaaattgat attgcaatag
    12241 acctgctttg attcttagtt tgttccatac ttaactttcc attcatctta aatcctgcca
    12301 atgggaataa aaatataacc agtgcatcaa ttccaagtac aacttgggct gcaatagtcc
    12361 atttgctcaa aaagcagaat attccaccca cacctaaaag aacctctaat atatttataa
    12421 acttatatgt aggttttttt gctatatatt cttcaatttt ttccttacat tctgcacgac
    12481 atggatatat attcgtattg tcatcatatt ttatagataa attattattt ggtgacttta
    12541 tatccctatt acagtaaaga cattttttca ttttatattc tcctctagta ttccttaaaa
    12601 aattttgcct aaattattat acgctataat actattatac caaatagata caaaaaacaa
    12661 aaataaaagg aataatttta gctatttctt ttatttcaat gtgtagtcta taataaattt
    12721 ataataacaa ttaaataata ttatttagtc taatttaatt gttatgtaat ttaaaataag
    12781 tcaaatttgt atgagaggtt aaaatgaaac aaatagaatt agctattgta agtttaaaaa
    12841 aagacgctgg agaaatttat gaaaatcaaa taaggcaatt cttaggtgat aatctaaaga
    12901 taaatttata ctcatttgaa gagggaaatt taaagttttt taaagaaaaa cttatactcc
    12961 tatctgctta cttaaaatat gatgaaatag taaaattatc tcactatgat gctcaaatca
    13021 ttgttcccaa gctgacattt gaaaagaaca gtattgatat gatatccaaa ttagaaaaag
    13081 ataaaattat ttatgtatat aacttaagta aagatatggc aatagaaaca atttctttaa
    13141 tacatagact aggaatagac aatatcaata tcctgccctg ctatccagag attgagttta
    13201 ctccctcaga tgctataatc ctaactcctg gtgaaaaaat cttgcctaag tttaaaaatt
    13261 gtgaagttat agatttaaaa tacagaataa tagatttaag ttgtattgtt gaaattgcta
    13321 caaaaaccaa attaaaacac ctaataaaag atgagctaat aaaaaaatac gtagaaaaaa
    13381 taattcctac aagttatagt acaggtgaat tattattaga tgcaaataaa tttgaaagac
    13441 aatttgatta aaaaatagct acgaacactt gtgactttag tcatgagtta gtagctaaat
    13501 tagtcagcat atagggaaac ctatatgtag agataggata gaacctatcc aatactactt
    13561 gaattgctg
//
LOCUS       DAMJVM010000093        13386 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.097, whole genome shotgun sequence.
ACCESSION   DAMJVM010000093 DAMJVM010000000
VERSION     DAMJVM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13386
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.097"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            212..1288
                     /locus_tag="PC523_002829"
     CDS             212..1288
                     /locus_tag="PC523_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2476334.1"
                     /translation="MKTQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(1391..2233)
                     /locus_tag="PC523_002830"
     CDS             complement(1391..2233)
                     /locus_tag="PC523_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2476335.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNKTFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKIEVIDSLSGSIGIGQLVLKAAILIEEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEVGSVMGTYTSKGAIL
                     ITAI"
     gene            2417..2989
                     /locus_tag="PC523_002831"
     CDS             2417..2989
                     /locus_tag="PC523_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2476336.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKNDIIYDIADTHLEVITSAILNCVKNVKSKNSQIDMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERMRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLINHWI"
     gene            complement(3152..3424)
                     /locus_tag="PC523_002832"
     CDS             complement(3152..3424)
                     /locus_tag="PC523_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476337.1"
                     /translation="MLRERFVVRNHHNPHTSECCGTGGKGCCGGDNNEHSHQHSHSHG
                     HEHEHINGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDEHTHEHK"
     gene            complement(3538..4410)
                     /locus_tag="PC523_002833"
     CDS             complement(3538..4410)
                     /locus_tag="PC523_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2476338.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIVAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(4697..7804)
                     /locus_tag="PC523_002834"
     CDS             complement(4697..7804)
                     /locus_tag="PC523_002834"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDF2476339.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEIYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAERAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVVKK"
     misc_binding    complement(7896..8128)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8303..8830)
                     /locus_tag="PC523_002835"
     CDS             complement(8303..8830)
                     /locus_tag="PC523_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDF2476340.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCKENTA
                     LREKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(8865..9650)
                     /locus_tag="PC523_002836"
     CDS             complement(8865..9650)
                     /locus_tag="PC523_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDF2476341.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITRKGKIKVQARLIV"
     gene            complement(9660..9917)
                     /locus_tag="PC523_002837"
     CDS             complement(9660..9917)
                     /locus_tag="PC523_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDF2476342.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(9936..10394)
                     /locus_tag="PC523_002838"
     CDS             complement(9936..10394)
                     /locus_tag="PC523_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDF2476343.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(10433..11137)
                     /locus_tag="PC523_002839"
     CDS             complement(10433..11137)
                     /locus_tag="PC523_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDF2476344.1"
                     /translation="MGSIKDNLSEIRSKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGNKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYDKK"
     gene            complement(11147..11782)
                     /locus_tag="PC523_002840"
     CDS             complement(11147..11782)
                     /locus_tag="PC523_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476345.1"
                     /translation="MKKVKYTRLVILGIFIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGAIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYREQKIDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVINEQSQSV
                     KFVILEGITYENESSVFVDYYKNDLNGVTSVNLYDKIILRPNSINTNIRIK"
     gene            12162..13202
                     /locus_tag="PC523_002841"
     CDS             12162..13202
                     /locus_tag="PC523_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2476346.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
BASE COUNT         4280 a         2261 c         1572 g         5273 t
ORIGIN      
        1 cataacttta tagacttaat ttattacttt tatagaattt tcacaataaa aagtcatttt
       61 acttctgtat ttaatgcaaa aacagagatt aaacttaata taaaaaatat ataacaaaat
      121 ttttttatat ttctaaaaga aaagtatact cttttttgtt taataatgct aaaattgatt
      181 tgtattgaat tttgattagg aggaaattat tatgaaaact caaagattaa atgctgtact
      241 tgaacaaatg aaaaaagatg atatttctca aatgttagta agtgacccta cttcgatatt
      301 ttatcttaca ggggtactaa tccatccagg tgaaagatta ctagcattat acttaaattt
      361 aaatggcaat aataaattat ttataaatga attattccca gtttcagaag atttaggtgt
      421 tgaaatggtt tggtttaatg atactcaaaa tccagtagaa ataataactg aacatataga
      481 caaaaatgct actatgggtg ttgataaaaa ctggccagct agattcttat taaacttaat
      541 agaattaggt ggaggaagta agtttgtaaa ttcatcttat ataatagata ctttaagaat
      601 gtgtaaagat gaagaagaaa aagagttaat gagaatagct tcaaaattaa atgacaaggc
      661 tatggaacaa cttaaagcta ctgtttctgg tgaacttaca gagaaacaac ttgttggtaa
      721 attatctaaa atatatgaag acctaggtac tgatggattt tcatttgacc ctattatagg
      781 ttttggtcct aatggagcta atccacatgg tgaacctgga aatgcacttg tgaaaccagg
      841 agatgcaata atattagata taggttgtat aaaagataac tattgtgctg atatgacaag
      901 aactgtattc tataaagaaa tacctgaaaa aggtagagaa atatttgaaa tagttcttga
      961 agctaataag agagctgaag ccattgtaaa accaggagtt agattctgtg atatagatgc
     1021 agctgctcgt gattacataa ctgaaaaggg atatggacaa tactttactc atagaactgg
     1081 tcactctata gggcttgaag ttcatgataa aggagatgta tcttctataa atacagatac
     1141 tgtacaacca ggaatgatat tctctataga accaggtata tatttaccag gtgagtttgg
     1201 agttagaata gaagatttag tacttgttac tgaagatggt tgtgagatat taaacaaaca
     1261 cgacaaagaa atatgtgtag ttggttaatt ctaaaatcaa tttgataaaa ttaaaaaacc
     1321 atataaaaat gtttaaaaca tttttatatg gttttaattt ctcaatctat gatttatttc
     1381 aatccatcat ttatattgca gttattaata ttgcaccttt tgaagtatat gtacccatca
     1441 ctgaaccaac ttcacatatt acaacatctt tatattttac atccttttcc ataacttccc
     1501 ttaccttttg agccttttca gggcaattag catgtcctat aaataaaacc ttttcttctt
     1561 tatctttaat ttttgactct actaaagtaa tcagcttttt caaactattt ccttcgcctc
     1621 ttgccttatc tataggagtt acaacgccat ctgcaacttg tattatcgcc ttaaaattca
     1681 aggtatttat aatttttcct gctattggat ttatcctacc acccttaata gcattttcta
     1741 aagtttcaag agttccaaaa aatggaggat tatctttata tttatttatt attgaaacaa
     1801 tctcttcaat ttcttttcct tcttctatta atattgccgc ttttaacact aattgtccta
     1861 ttccaattga cccactaagt gaatctatga cctcaatttt cttgttattt ccttcttttt
     1921 caaacatatc ttttgctaaa agtgcactat tataagtccc agataatcct gaacttatat
     1981 taattaccaa tacactttct tcctgacaat ggtatgcttc taaaaattta tctggagaag
     2041 gacatgatgt ttttggtagt actttgctct cactcatttt tctatagaat gttttattat
     2101 ctatttcctt tccagatata taactttctt ctccaaaaga tacatttaaa ccaacaactt
     2161 ctatgttgta tttttctatt atatcatctg gtaagtcaca tgcactgtcc actatcaatt
     2221 ttattttatc catattaacc tcactataag atattatttt atttattcag tcggtcgtac
     2281 gaccgattta attattattt tatatattga atgaaaaaaa gtcaatagat atagattctc
     2341 cctttattga caataaatat tcataagctt ataatgttat taatttgtta taagtataca
     2401 tggagaggta ggttttatga aaacaaataa tatgaaacag gctattttat catctgcaac
     2461 taatttaata gccaaaaatg gagttcaaaa tactagcctt gctgatattg caaaagatgt
     2521 aaatataagt aaaggcactt tgtattatca ctacgcatct aaaaatgata ttatatatga
     2581 tattgcggat actcatttag aagttataac tagtgctata ctaaactgtg taaaaaatgt
     2641 caaatcaaaa aattctcaaa tagatatggt taacttaatc ttagagaaaa tctctactat
     2701 agaaagtcgt ggtagggtac atatgtatct tatttgtgag gcaattactt caaataatga
     2761 tttaaaagaa agaatgagat taaaatatat tgaatggaga actactctta aatctgaaat
     2821 tgttgaatca ctagaaaaat ataatagtag cgaaaatgaa caggatgctg aatcattttc
     2881 ttttttactc atgtcaatag ttgatggttt agttgtccaa agtttattaa aaacagaaaa
     2941 aataccttat gaaaatatag cttctttttt aattaatcat tggatttaaa tttaatactt
     3001 agataattaa taaaagtgaa ctatttaagt tatctactct aacagttcac tttcttattt
     3061 attatcaata aattttactt aaaaccttca atttttattt aaaccttcat ggaatcacaa
     3121 aataaaaatc tcttataaat ttaaaatgtt tttatttatg ttcatgtgta tgctcatctg
     3181 aatgttcatg accatgtgta tgtgtatgtt catggctatg ctcatgagta cctgaaaaac
     3241 catcatcatg attatggtcg tgacctacac catttatatg ctcatgttca tgaccatgac
     3301 tgtgagaatg ttgatgtgaa tgttcattgt tatctcctcc acagcatcct tttcctccag
     3361 tcccacaaca ttcacttgta tgtggattat gatgatttct tactacaaat ctctctctta
     3421 acataattct tatacctcct agtatattat tgagtacttc atgtactcca ttaattacag
     3481 tatacatcta actaggtata atctcaatac atatggtatt ttttaacatc tatagtatta
     3541 aagaaactta tccttaaaac ctccaccttt tacttcttca acattattga cagaaataaa
     3601 agcttcacta tccatatcat aaattaaatc tttaaattta cctatttcat ttgcagatat
     3661 tatgcaataa atcatctttt tcttattctg tgtatatgca ccttctgcct ctaaaaaagt
     3721 gactcctctt cccattttat tcattattac tttagatatt tcatcatatt tatttgaaat
     3781 taaaagtatg gatttttttc tatcaaagca atcttttcct atatccatag taaaagaaat
     3841 tatatacatt gtaattaaag tgtacatcac caatttagct ccaaataaga aagcccctgc
     3901 acatactaca aaaaaattaa taaataaaaa tgtatttttc ataggtatat catactttct
     3961 tttcattata gctgctacta tatcaagtcc tccagcagta gctcttgctt taaaagtcaa
     4021 tcccatgcct ataccagtta atgcaccacc ataaatacac tgtagcatag tatcatctat
     4081 cgggaaatat ttagcaatat cttgtgttaa cccaagtgct aaagaaaata gtaacatatt
     4141 gacaaaactt atcaataaaa acttattatc aaaatacttt ctagctataa taaatattgg
     4201 tatgttcatc aagaaacttg atattccctg atttatgcca aacaatttat ataatataac
     4261 acaaattcca gctactcctc cacttaataa tttggctggt gctagatata aatttattcc
     4321 taaggttgat agaatcatac ccaatataat aagacaatat tgtaaaaata tgttattatc
     4381 aatacttagt tttttgcttt caccatacat caaaacgcac ccctttgcat aataaataaa
     4441 ctaccctgca aaacacacga taaatttata aataaattta tcagaagtag cttactaaaa
     4501 atattattca tcctcactta aacgtttatt cattaatatt ctatcacatg atatgaataa
     4561 tttatatgat ttatatagaa ttatttattg taaattttgg aatattaaaa cattttagtg
     4621 aatagataat tatattacac aaaaaatccc tatagaaatc tataaactaa tttctatagg
     4681 gattttgatt aactaactat ttttttacaa cttttatacc tgtcatatgg tcgtttaagt
     4741 tttgttcttc taaagattta tcatctactc tgtttatttc taaagctaaa gtttcagact
     4801 tgatgtattc attaaaatgg tcaacagcct tagcaatttc gtcatcacca cagtattcta
     4861 ttactatatt atctaatact tcaaagttat tagattttct caattgctgt actttagata
     4921 taaattctct agcataacct tcattaatca actcttcagt tagcttagta tctagtataa
     4981 caaaaagatt gttttcaaca gatacattaa atccttcctt agcagttata cctattaata
     5041 ctaaatcctt attaaattca aaagcttcac catctaaatc aactgttaga gtctcacctt
     5101 tttcaagttt attagcagtt tcatgagcat ttaatttact taaagctcca acgaataaat
     5161 tcatcttaga acctaataat ggtcctgctt ccttaaagtt tggttttaaa ctaaagttca
     5221 tatactcatc taaatcctta gcaaagatta cctctttaac attaagctct tcttttatta
     5281 aatcaactac atcgcttatt gtatcttcaa acttaccatc tactaaaact ttttgtaatg
     5341 gttgacgaac ttttattctt tcaacttctc ttgaagctct acctaaagta actaagtttt
     5401 tagctaaatc cattttttct tctaatttag tatccactaa ctcttcatta cacttaggat
     5461 attcagctaa atgaacagat acttctcctg ttaagtttct atacatttcc tctgacatat
     5521 atggtgcaaa tggagctata gctctacaaa gaccatgtaa caattcataa gttgtgttgt
     5581 acactgcttt cttatcttct gttaattcag ttgcccagaa tcttcttctt gaacgtctta
     5641 tataccagtt agataaatca tcatttataa aatcttgtat cattcttaaa gttttattta
     5701 actcaaatat agctaaattt tcttctacat ctttcattaa attattgaat ttagataata
     5761 tccatctatc aagttctggt ctatctttat attctacaaa aaattcagtt ggatttatat
     5821 catctgtatt tgcatataaa gtaaagaaat tatatacatt cttcatagta cctataaatt
     5881 tactttggat ttcttttaat ccatccatat caaatttagt tggagtccat ggtggagaaa
     5941 catatagtaa ataccatctt aatgcatctg ctccatattg gtcaaataat tccattggat
     6001 ttactgtgtt tcctcttgat ttactcattt tcttaccatc tttatcaagt acaaggtctg
     6061 gaactaaaac actcttatat ggtgctttcc ctaatacaaa tgttgatact gcaagtaatg
     6121 agtagaacca tcctctagtc tggtctatac cttctgatat ataatcagct gggaatagtt
     6181 ggtcaaagtt ttccttattt tcaaatggat agtggtgttg agcaaatggc atagccccac
     6241 tatcaaacca acagtctata acttctgtta ctcttgtcat tggttttcca catttttcac
     6301 attttaagtg tatatcatct acatatggtc tatgaagttc aactgtttct ggatttacat
     6361 cttctatagc tctttcagct aattcagctc ttgaaccaac tgaatctgta tgaccacatt
     6421 cacatctcca tataggaagt ggagttcccc aatatctagt tcttgatatt gcccagtcat
     6481 ttagattttc tagccagttt ccaaatctct tctctcctac aaaacttggg aaccatttta
     6541 caccattatt attttctatt aatttgtctt taagtttagt catctctata taccagcttg
     6601 gttttgcata gtatactaat ggagtttgac atctccagca gtgtggataa ttatgaacta
     6661 ctttttcttt gctatataat ttattttcag aagctagcca ttttattatt tcaacatcta
     6721 gcccatcttc cattacaaat ctaccttccc aaggagtaga tgtaaattta ccagtttcat
     6781 ctactggttg aataactggt aaatcatatc ttttacctgt gttgtagtca tcttcaccaa
     6841 atgctggtgc tgtatgaact ataccagtac catcttcagt agttacataa tctcctactg
     6901 taataaaaaa tgcttttttg tcaacttcta caaaaggcat taattgttca tattccaaac
     6961 cttctaggtc tttacctttc atttcttcta aaacttcata atcctcacct agtactttat
     7021 cagctaaagg tttagataca taatatatct catcaccttt tttaactttt aaataatcta
     7081 catcagcatt tactgttaac gaaacatttg atggtaaagt ccatggagtt gttgtccatg
     7141 ctaagaaata ttcatcagca tcttttttct taaatttagc tataactgta ttagttttta
     7201 cttctttata tccttgtgca acctcatgac tagcaagacc agttccacat cttggacaat
     7261 aaggaagtat tttgtgacct tcatagatat atccttcttt gttgaattta ttcagtatcc
     7321 accatacaga ctctatatag ttattatcta aagttatata tggattgtct aaatcaactt
     7381 cataagccat tctctcagtc attattctcc attgtttttc aaatgagaaa actgattctc
     7441 tacatttttg gttaaattca gctattccat actcctctat ttgttgttta ctagtaagac
     7501 ctaattcttt ttctacttgt atttcaactg gtaatccatg tgtatcccaa ccagcttttc
     7561 tttttacttg ataaccagac attgttttat atctacaaac tgaatcttta agtgttctag
     7621 ataaaacatg gtgtactcca ggatttccat tagctgttgg aggtccctca taaaatacga
     7681 aacttgggtc atctttccct ttttctaaag ttttttcaag tatatttata tctttccaat
     7741 aatcaaaaac ttgtgcttct gcttgtttta cagaactgtc taccaatggc ttaaatttcg
     7801 ccatgtaaat caccctttcc tataaatatt ataattccaa aattttttat tctttcacat
     7861 aaatttaaat actaaaaaac tcgccccctt taaaccaaag ggacgagtta taaatcgcgt
     7921 taccacccta attctatatg taaactaaat actagctaca tataacactc taatagtata
     7981 acggtactaa ccggcacagc ttagtagaat ttaatcgttc agcctgcagc tcaagagtga
     8041 tttttacctt tataaattca ttggttctca gcatacccaa ttctctgtaa aattatctaa
     8101 agataaacgt ctctatcaac gcttttaaaa atttatctgt gtttatatga aattctataa
     8161 caaataattt gaatagtcaa tatatttttg aaaaatataa taatttctat ttttcttaca
     8221 tttttatcct atttttaatt tttgttatag atttctcaaa cttacacaat gattctacat
     8281 aacaaataac caaattacaa gcttattcta aagttgtagc agcttcatca ttactataat
     8341 tatacactgt tgttccttca aatgcattta cacatttttc atctacatca taaaaaatct
     8401 catctacact tcttatttca tcttctaaca atgatttgaa tttatttcta aatatcttaa
     8461 attctttgac taaagcatca tactcttttc ttatctctat aacacggtta ttagcttgtt
     8521 ctataatttg tctagctttt aaatcagctt cttcaacaat tatcttagcc tttttattag
     8581 ccgcgctaca agtgtcttct gctgcacttt gagcagttat taaagtagct tttagtgttt
     8641 cttcaatatt ttcatactta tttacttggt cttgatataa ccctaatttt tctctaagag
     8701 ctgtattttc cttacataga gactcataat cttccttgat gatatctaaa aattcatcca
     8761 cctcttcatc tctatatcct cttaaacctt ttttaaactc tttattttct atctcaattg
     8821 gagttagcat tacatttcct cccagctata atttaaatta gtccttatac aattaatctt
     8881 gcttgaactt ttatttttcc ttttctagta atatctccta tttgtgttac cttagctctt
     8941 cctttacctc taatagaaat caaatcatca ttttttacta ttttagaagt tgaagttatc
     9001 ttttcataat tgacatggac tttttctcca tttatgtatt ttgcactatc ttgtctagat
     9061 atattgtaaa taccacttat cacacaatct agcctatctg aagatactgt aaatgacact
     9121 tctttatatt ttgaagaact attaacaata ttttctctgg aaatttcctt tacaattaca
     9181 ttatttcttg aaactttttc taagtttaca attataaaat cgcaaatatc actacttaca
     9241 actacttgac aaaagctatc atgtataatt atatctccaa ttttttctct ttttatacct
     9301 agacctagta ttgaccctaa gtaatcttta tgagatatat ttttaaattt aaaattccct
     9361 tctatttgta aaaatctaag agtatcttct atatcttcat attccatgta aaatggataa
     9421 ataaatacag tgcttctttc agcttgttca taacctccat ccacactgta ttttatttca
     9481 tttgtagaat ttaatattgc tacagcattt ttcacttcat atggattaag aaactccgta
     9541 gacttaacat catagttttt tatacaacta ttagccttgt ctatcacttt aaacatttta
     9601 tttttcaagt ctatatcctt tatgtgattt gttagtttta atttatccat cctatcacct
     9661 tagaatataa ccaatactaa ttgtcttagg aacatcaata atactattgc aattaaagga
     9721 gaaaaatcta taggcccatt tgtatattta tacattatgg accttattgg cccttctatt
     9781 ggctctgtaa gattttctaa aatatcaaaa attttacttt gtcttccatt tggaaaccaa
     9841 gtcatcaagc tttttataac tatcatccat gctattatat ctaaaagata ataaagtgca
     9901 attcttattg ttcccataca ttaaccgcct ttttactatt tattttgcca agggaagaat
     9961 gctttacttt ctaattcttt tttcatattt gcatctatat ccacattatt aggagctagt
    10021 ataaatattg atttagaaac cttctgtata cttccatcta atacataaac tgccccattc
    10081 ataaagttaa atatagattt acgaacttca gaattagtca atccttccaa attcactata
    10141 actgcccttc tttgttttag atggtctgct atagttgcag cttcatcata aaccttaggt
    10201 tcaactatta caactttcat ttgactagtt gtatgtaaat ttacaatttt atttgctttt
    10261 gcagtactaa atccactgtt catttcttct tcttgaacta tatcgtccat accactttca
    10321 tattcatctt caatgtaatc ttcttcttca tcaactatcc aattcttaaa tttagatatt
    10381 attccatttg acattattaa cttcctcctt aaaatttatt caatttgagt aattattttt
    10441 tatcgtagtt tctttctcca aagattgatg ttccaactct aacaatagtg gccccttcct
    10501 ctatagcaat ttcataatcg tgactcattc ccattgataa aacatccatt cctacattag
    10561 ggatatttag ttcttttatt tgttttgata aatctttaag acctttaaat acacctctta
    10621 tctcatcttc atcttctata aaaggtgcca ttgtcattag accttttatt tttatatttt
    10681 tatatttttc tgacacttct ttgacaaagt ctacaacttc ttctctatca agtccatgct
    10741 tagattcttc tttagatata ttaacttgta ctaagcaatc tatttctctg tttatttgct
    10801 ctgcacgttt ttgaatttct tcgcataatg caattctatc taaagagtgt atcatacaaa
    10861 ctttatcgat tatgtactta actttattgg tttgcaaagt tcctatcaaa tgccagttta
    10921 atttgttacc tataatatca tactttctag ctaattcttg tggtttattt tcaccaacag
    10981 aagtaattcc ttccctcatt gcctcattaa ctaagtccac atcaacagtc tttgtaactg
    11041 ccaaaagggt aatctccttg ctatctctat ctactttttt tgcagcttta tcaattttgc
    11101 ttcttatctc agacaaattg tctttaatag aacccattat tatcacctac tttatcctta
    11161 tatttgtgtt tatactgtta ggtcttaaaa ttattttgtc atatagatta acacttgtaa
    11221 ctccatttag gtcattttta taataatcca caaatacaga agattcattc tcataagtta
    11281 ttccttctaa tatgacaaat tttacacttt gtgattgttc attaattaca tatacaccac
    11341 gtttattgtc ttttgttttt atagaagttt taggtatttt taaacattcc atctgattat
    11401 atattatatc aaattcttcg acacttgtat cctgatttgc aacaatttgg ttacttattt
    11461 ttaattttac cattatttta ttattatttt catatatttt gtaaactttt gcatctattt
    11521 tttgctcacg atattcaatt attacatttt gatttttttc aaagttatta gaatcttctt
    11581 tactgatgta tgtagataga taattgcttt ggtcattggc atctttttct atagctcctt
    11641 ttacatgaat ctttgcatcg actttttcat tttttaacac caatgtatct acatttttgc
    11701 taattatcat tactaatata ttaaatacta aatatataaa aataccaagt atcactaatc
    11761 tggtatattt aacctttttc aaagactctc cccctataaa atttctttca ttataagtac
    11821 ttcttctaaa aaatgtttat tcctttttta taacctaata ttttacattc taataattat
    11881 tttttccata ttaaatttat tttttatcaa tcttcctata caagactgca ttatttatat
    11941 ataattatat tataaagtat aagtttaatt tacattttat acgttaagct tactttatat
    12001 gttttatagt tatttttatt tttagatgca atataaaacc aattaagatt taaaataact
    12061 tttgttctgt tgacatttat ttaggtaatt atttatgcat taggataacg ttgtcacaac
    12121 aaaagtttat gtcctaaaaa aaatacaagg aggattttct tatgtcagaa attttattag
    12181 gaacaggttt acttttattg gttctatgtt gtttcacaat ttttagttac aaggctccat
    12241 atggtatgaa ggctatggga gctttagcaa acgctgcatg tgctactttc ttagtagagg
    12301 cattcaacct ttctttactt ggtgatgttc tgggtatgga attttttaga agtgtcggag
    12361 ctgctaatgg tagtcttggt ggagttgctg ctgctatact tgtgccttta gctttaggtg
    12421 tatctccagt ttatgcagtt ctaataggat tatcttgttt agatttttct atattatctg
    12481 gattcgttgc tggatacttt gtttctttct taattaaatt tatagagaaa aaagtaccac
    12541 ctggagtgga tttagtggtt gtaattcttc tatgtgcacc actatctcgt cttatagcat
    12601 caaatgtaga cccattagtt aatagtacac tactacaaat tggacaagtt ttgatagaag
    12661 ctacaaacat tagccctata ataatgggaa ttatactagg aggtctaata acagtggttg
    12721 gaacatctcc acttagttca atggctctta catctataat tggtcttact ggccttccta
    12781 tggcgatagg agcattaggt gcttttggtt cagcttttat gaactcaacg ctatttgcaa
    12841 aattgaaatt aggttctaaa aaagatatta tatctgttgc tattgaacca ttgactcaat
    12901 cagacataat ttcagctaat ccaataccta tatattctac taatttcttt ggaggagcct
    12961 tttctggtat cattgtagcc ttgatgggtc ttgtaaacat gactccaggt acagctacac
    13021 ctgtggctgg ttttgctata atgtttgctt ataatccagt tcaacaagtt cttataacag
    13081 ctggtttatg tgctttattc aatgtaattg caggattttt aggttcaaaa atattcagta
    13141 cctttaaagt tcttactgct ggtgaaatac gtggtactga tgtagaagaa gaaattgctt
    13201 aaaaaaacaa taagatttat aaatttatat ctcaagtagt agagttttat attgtaggca
    13261 attttatcaa ttgcaaacgt tttatttttt atttatgttt atgattatta tgtgtgttga
    13321 atatttagat aaatttttat ttattatttt tgtttagttt tttatttggt tgatatatta
    13381 ggaata
//
LOCUS       DAMJVM010000094        13279 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.098, whole genome shotgun sequence.
ACCESSION   DAMJVM010000094 DAMJVM010000000
VERSION     DAMJVM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13279
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.098"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(88..2088)
                     /locus_tag="PC523_002842"
     CDS             complement(88..2088)
                     /locus_tag="PC523_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2476347.1"
                     /translation="MKQSIAIVTDKLTKLSSFLEENIRLVLGNYIDINHYYLEVLKED
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKEKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGVINTFNKKALKILGINENPQGKKIEDIISKDLNILLGDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMIKCIELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLPKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYISVEFANNYEDGFGYTKKEFKEILRILYERRESSSGVGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            complement(2119..3837)
                     /locus_tag="PC523_002843"
     CDS             complement(2119..3837)
                     /locus_tag="PC523_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2476348.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIVLFIKYDQNGLKEREIISKG
                     KYMSESLVADKSYDEAKKVVKTSFESIPAKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDAKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASILLKVGSYKTGEKLIKDSFKIDIDEGEKNGMVRLYALINLAEIYVEVGEY
                     DKAIEISSRVKDYKKFVNTDNYNDFEIMASIMQANAYIEKNNIQEAKNLLEKADYIIK
                     SDRTVHILDKDMYYYMAMGNLEYKNENYDSAIDNYKKSLDISTKRKLTQEKIKNLNKI
                     LNIYEKQGNVESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESELFEKTKKEIK
                     YYKILFTVILIAVLGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYRAKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDVVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGEEVADIVDNIRVAIANKKWNEDVTVTISAGVAHISDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            complement(4056..5294)
                     /gene="fabF"
                     /locus_tag="PC523_002844"
     CDS             complement(4056..5294)
                     /gene="fabF"
                     /locus_tag="PC523_002844"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDF2476349.1"
                     /translation="MNKRVVITGLGCVTPLGTGREEFWSNIKAGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAVVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            complement(5323..5547)
                     /gene="acpP"
                     /locus_tag="PC523_002845"
     CDS             complement(5323..5547)
                     /gene="acpP"
                     /locus_tag="PC523_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDF2476350.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            complement(5610..6359)
                     /gene="fabG"
                     /locus_tag="PC523_002846"
     CDS             complement(5610..6359)
                     /gene="fabG"
                     /locus_tag="PC523_002846"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDF2476351.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIENMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMASYITGQVINVDGGMVMQ"
     gene            complement(6359..7309)
                     /gene="fabD"
                     /locus_tag="PC523_002847"
     CDS             complement(6359..7309)
                     /gene="fabD"
                     /locus_tag="PC523_002847"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDF2476352.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNTVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAEIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPTGVGAMAAILGLDRNALESVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAIVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NMTKPKVDVVANVNADYYKNEGKELLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            complement(7325..8254)
                     /gene="fabK"
                     /locus_tag="PC523_002848"
     CDS             complement(7325..8254)
                     /gene="fabK"
                     /locus_tag="PC523_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HDF2476353.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            complement(8363..9349)
                     /locus_tag="PC523_002849"
     CDS             complement(8363..9349)
                     /locus_tag="PC523_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDF2476354.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVSEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            complement(9375..10397)
                     /gene="plsX"
                     /locus_tag="PC523_002850"
     CDS             complement(9375..10397)
                     /gene="plsX"
                     /locus_tag="PC523_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDF2476355.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVSLNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDIKQFISKYNKEN
                     KNNEDE"
     gene            complement(10408..10965)
                     /gene="fapR"
                     /locus_tag="PC523_002851"
     CDS             complement(10408..10965)
                     /gene="fapR"
                     /locus_tag="PC523_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HDF2476356.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            complement(11172..11348)
                     /gene="rpmF"
                     /locus_tag="PC523_002852"
     CDS             complement(11172..11348)
                     /gene="rpmF"
                     /locus_tag="PC523_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDF2476357.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            complement(11373..11897)
                     /locus_tag="PC523_002853"
     CDS             complement(11373..11897)
                     /locus_tag="PC523_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDF2476358.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            complement(11990..13186)
                     /locus_tag="PC523_002854"
     CDS             complement(11990..13186)
                     /locus_tag="PC523_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDF2476359.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEDALKECIDLAPLHNPANIMGIDACKAILPDVPMVAVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
BASE COUNT         3936 a         2627 c         1354 g         5362 t
ORIGIN      
        1 aatataaatt aatttctata aatataaaag tttattattt aaatatctaa attcaaaata
       61 tgtaatttca atatcaatat acttaactta atttatatta ttataaagtt ttataccctc
      121 tgaggttatt tgacttccac ctctaccaga tttagatgat atatatccac attcacttaa
      181 ataagatagc acctttctaa ctttgctttc agaaatctca atcccttcct gtataagctt
      241 ttcaattata aaccctcttc caactccaga agacgactct cttctttcat atagaatcct
      301 tagtatttcc ttaaattctt tttttgtata gccaaatcca tcttcataat tattagcaaa
      361 ttctacactt atatatggtg gtaagtcatc cctaaaaacc atactatcac acattagact
      421 tacgtaagaa gcaacatttt gtaactctct tatatttcct ggccaattat atttttctaa
      481 aatagttata acttctggtg aaagtcttat attatttttc aaaaagaagt tcataagtgt
      541 tattatatct tcttttctat tcctcaatga tggcacatct attggtaata catttaatct
      601 ataatacaag tcttctctaa attttccttc tctaatcatc tttggtaagt ttttgtttgt
      661 tgctgctatg accctaacat ctatatttat aacattatgg gaacctatag gcataacctg
      721 cttttcttgt aataccctta gtaactttgt ctgtagcaat ggaggcatat ctccaatttc
      781 atccagaaat atagttccat tattagcaag ttcaaataac ccctttttcc cttctcttaa
      841 agctcctgta aaagagcctt tttcatatcc aaacagttga ctttctaata gactatctgg
      901 aactgctgca caattaactg ctataaacgg ttgattactc ctattagaag cattatgaat
      961 tgattgcgat aataattctt taccagtacc actttctcct attattagca cagaaaggtc
     1021 tgatttagat atttttttag ccaattctat acactttatc atatttttat ttttagtttt
     1081 tatatcttta aacgtatatc ttgctatctg cccttgttct cttaatttat tagtaaggtt
     1141 ttgttctaat ttttttatat acgttatttc ttgcaagtta taatatatac cagctttatt
     1201 ccccatagaa aaaatacttt ttttatttac atttatatat tttttatttg taaatacaac
     1261 ttcatcaatt atttctttgt ctccaagtaa aatatttaaa tctttagaaa tgatatcttc
     1321 tattttttta ccttgcggat tttcatttat acctaaaatt tttaaagctt ttttattaaa
     1381 tgtgtttatt acaccatctg ctgaagtaaa tattatacca tcttttgata gattcaatat
     1441 aacatcttgt tcttctattt ttaaaaacag ttgtctataa ttatctttaa ctccattatc
     1501 taaacttatt aaactctcag aatatgccat taaattttta cgtatttctt taacatcaat
     1561 tctaagacca tttattattt ctataaaggt agatatatcg atatatctat taccaaggtc
     1621 gattacttta tttatataac ttggtacttt ttcctttact ccaggcgtta tagctatgtc
     1681 tatattttta taaatttttc cttcaatata tggagtaaac tttaaatttt ttactcctat
     1741 tttataaaat aaacttattg tttctaaggt agtttccttt gtatcattta caaccaatac
     1801 ttcagttcca tttggtattg aaaataatcc atatatttca ttttctttaa gggttctcct
     1861 tatcactata atcttactat cttgtactaa atgttttctc atactgttta atctatcatc
     1921 attcattact agtacaaaat caccttgtat tttatcatcc tctttcaaaa cttctagata
     1981 ataatggttt atgtcaatat aatttcctaa aaccaaacgt atattttctt ctaagaaaga
     2041 tgatagttta gttaatttat cagttactat agctattgat tgtttcatac ttatacctct
     2101 tatttatata gtatacagtt acttatatac tactttattc ctaccaagtt gttttgcttt
     2161 atataaattt ttatcagctt cttctagtac atctttgcta tccgaaatat gagctacacc
     2221 tgcacttata gtcacagtta catcttcatt ccatttttta tttgcaattg caactcttat
     2281 attgtcaact atatctgcta cttcttcacc agatttattt cttatcaata cagaaaattc
     2341 ttctcctcca tacctaaaaa ctttatcatc ttgttccaac ataacacaaa tagtatttgt
     2401 tatccctttt ataacaacat caccaaattt atgtccataa ttatcattta tgagtttgaa
     2461 attatcaata tctatcatta caagtgcaaa attatcatct ttagctctat acttttcata
     2521 cataatatca aagctttttc tattataaac atttgtaagc ccatcatgta aattttgtgc
     2581 tttaaaatac ttcaatctgt tgtataaaaa agtagaccct aatacagcaa ttaaaattac
     2641 tgtaaataat attttataat attttatttc ttttttagtt ttttcaaata gctcactttc
     2701 atttgatact ttatcaatta tatagaaact agtctctgaa tctcttatac ttttgttctc
     2761 tttatattca tttattaaca tcttttgata ctcatttaaa gattctacat ttccttgttt
     2821 ttcatatata tttaatatct tatttaaatt ttttattttt tcttgtgtta gctttctttt
     2881 ggttgatata tctaaacttt ttttataatt atctattgca gaatcataat tttcattttt
     2941 atattctaaa tttcccattg ccatataata gtacatatct ttatctaata tatgaactgt
     3001 tctatctgat tttataatat aatcagcttt ttctaaaaga ttctttgcct cttgtatatt
     3061 atttttttct atataagcat ttgcctgcat tatagaagcc attatctcaa aatcattgta
     3121 attatctgta tttacaaatt ttttataatc ttttactctg ctacttattt caatagcctt
     3181 atcatattct cctacctcta catatatttc tgccagattt ataagagcat ataatctgac
     3241 cattccattt ttttcacctt catcaatatc tattttaaaa gaatccttta ttaatttttc
     3301 tcctgtttta taactaccta cctttaaaag tatgctcgcc aaatcaacat ctagtcttgc
     3361 tttttcataa ttattcccca atttctcagc caattttata gactttacag ttaaatctac
     3421 tgctttagaa taattattat ccattatata cactgatgct aatctttttt ctatattaaa
     3481 cctgcactca tcagataatt ttgcatctga ttctctcatc ttttctaaag aatcaatgac
     3541 tatcttcgtt ccatctggaa catatgaaat agtctttaac atatcccaaa cttcattata
     3601 tactttattg tatttttttg ctggaataga ctcaaaagaa gtcttaacca ctttttttgc
     3661 ttcatcatat gacttatctg ccacaagact ttcagacata tattttccct tacttataat
     3721 ttctctttct ttcaatccat tttggtcata ctttataaat aaaacaatac taataagtat
     3781 tatacatata aaaattatct ttttaggtaa ccttttcttt ggtagtctaa aattcaaaat
     3841 tttagtcctc cttttgtata ttgtagaatg aaaacttgca tatataaata acctcttaat
     3901 aaataataat acaatatatc aaaatgattt acaacatatg ctagacttaa atcttaataa
     3961 aaaagtctcc taaaataatt taggagactt taaatttaat ccaatataaa tttatacagt
     4021 taaaataagt acttgttttt ttatttaata tttatttata catatttttt tagtactatt
     4081 gtagcattat gcccaccaaa tcctaaagaa tttgaaagtg catatctgat atttttttct
     4141 ttaccttgat ttggaacata atctaaatcc aaatcttcgc caacttcttg tatgtttata
     4201 gttggaggaa caaaactatc ttctatactc atagcacaaa caacagcttc tattgcacct
     4261 gatgctccta ataagtgacc cgtcatagat tttgttgatg acacacataa atcatatgcc
     4321 ttttccccaa atacagtttt tatagccata gtttcgtata aatcattgta ataagttgaa
     4381 gtcccatgag catttatata atcgacttcc tctagtggaa catttccatc tttcaaagcc
     4441 atattcatag acctagctgc tccctctcca ttttcagctg gagttgtcat atggtatgca
     4501 tcagcagtta atccatatcc aactacttct gcatatattt tagcacctct tgctaaagca
     4561 tgttctaagt cttctaaaat aacaatacct gctccttctc ccattacaaa accatctctg
     4621 tctttatcaa atggtcttga tgcagtttta gggtcatcat ttctagttga catagccttc
     4681 atattacaga atccagcaaa agcaagacct gttacagcag cttcagaacc tcctgctacc
     4741 ataatgtctg catcacctct ttgaattacc ttaaaagcat cacctattga atgagttcct
     4801 gatgcacaag ctgtacatac attagtgtta ggtccttttg ctcctaaaaa tattgaaact
     4861 tgaccagctg ccatatttcc tatcatcata ggtacaaaaa gtgaacttac tcttttattt
     4921 cctttttcca aaagaatttt atgttgagtt tctatagctt caactccacc tattccagaa
     4981 cctattataa caccaaaacg ttctctatca actttatcta aatcaagatt tgcatcttca
     5041 actgctaatt ttgcagatac tactgcaaat tgagcaaatt tatctaatct cttcaattct
     5101 tttttactta tatattcttc tggatgaaaa ttcttaactt ctcctgctat ctgagtttga
     5161 tacgtactag catcaaaatt agttatttta tctataccag atactccagc ttttatatta
     5221 ctccagaact cttctcttcc cgttcccaac ggtgtaacac atccaagccc tgttattact
     5281 actcttttat tcatcggata cctccacttt tttaaagctc gactatttat tagcttctat
     5341 atacttaact atatctccta tagatttaaa gttttctgct tctgtatctg gtatttctat
     5401 accaaattca tcttcaagtg ccattattat ttctactgca tctaatgaat ctgcttctaa
     5461 atccttcatt aatgatgtct ccatagttat ttctttatct tctactccta attgctctat
     5521 gattatttct acaactttat taaacattgt gtacctccta aaatttttat tttaatagat
     5581 ttctctactt tttaaattat tattttttat tattgcatta ccattccacc atctacattt
     5641 attacttgtc ctgttatata acttgccatg tcactaacta aaaatgctac agcattggct
     5701 acatcttctg gtttaccata agattttttt ggtatgtttg ctagtgttga ttcttttaca
     5761 tcatcactta gtactttagt catatctgtt tctataaacc caggtgctac tgcatttata
     5821 tttatgtttc ttgatgctaa ttctcttgca gaagcttttg taaatccaat tacacctgct
     5881 tttgatgcac aataattagt ttgccctgca ttccccataa ttccaactac tgaagtcata
     5941 tttattattc ttccatactt ctttttcatc ataggtttag taactgcttt tgtacaatta
     6001 aagacacctt ttaagtttat atctataact ctatcaaaat cttcttcttt cattctcata
     6061 agcaagccat ctttagttat gcctgcatta ttaaccaata tatcaatttt tccaaattcg
     6121 cttacaacag aatctatcat ttggttcact tcatcaaatt tagacacatc acattttata
     6181 gaagtacact ttacccccat attttctatt tcatttttag tttttagtgc ctcatcttct
     6241 ttagaagtat aattgattac tacatcagct ccaaaagatg ctagtttttt tgctatttct
     6301 tttcctatac ccctagagcc accagtaaca actgctactt gtcctgtaag atttatcatt
     6361 atatctctcc atttctataa ttttgtacaa attcattaag agaatctata ccatctatat
     6421 tataaatatt gacatttgct tttttatttg aagctatctt cttaataaat gcttttaaag
     6481 tttttccagg tcccatttct ataaaagtat catatccgtc atttaaaagc ctttctatag
     6541 aatcttccca aagaacagat gagcttactt gttttataag cagttcttta ccttcatttt
     6601 tatagtaatc tgcatttaca tttgccacca catcaacttt aggtttagtc atgttaactt
     6661 tttctaattc actagccagt tttactccag cttcttttaa cattgatgag tgaaaaggtc
     6721 cacttacatt taatttaact gccctttttg ctcctgcttc tttacatttc actattgctt
     6781 cttctatctt ctcattttca ccagatataa ctatttgtcc aggagaattg taatttgcta
     6841 cttctacaat accaccctgt atatctttaa ccacactttc aagtgcattt ctatctagac
     6901 ctaaaattgc tgccatagct ccaacacctg ttggaactgt ttcctgcata aacttccctc
     6961 tttttttaac taaacataca gcatctttaa agtcaattgc atctgctgca accaaagcac
     7021 tatactctcc taaagataat cctaagcatg cattatattt taaatctatt tcagcttgta
     7081 ctgcttttaa tactgctaca ctatgtgtaa ccatagcagg ttgagtgttt tcagttttag
     7141 aaagctcttc ttcatttcca ttaaacataa ggttttttaa gtccatgttt aaagcatcac
     7201 tggcctcatc tattacactt ttagcaacag tattattttc atataaatcc ttggccatac
     7261 ctacatattg agctccttga ccagggaata ctagtgcaac ttttcccatc ttgctcctcc
     7321 tgatttacag ttctattgct ggcataatct ctcttgcttg atttaccata gcttctataa
     7381 tttccttaca aggtgttatt tcttttacca tagcagcact ttgacctgcc ataaatgaac
     7441 ctttttctat gtctccatct actgttgcaa atcttaaagc tcctgtacct tttttatcta
     7501 attcttcagg agtagctcca ttttgttcca tctttaaaaa ttcttttgat aatttatttt
     7561 ttaatgttct tactggatga ccagtacttc ttcctgttac aattgcatct ctatcttttg
     7621 cttttagtac taggttttta tagtttgaat gtacagaaca ctcttcacta caaataaatc
     7681 tagttcctac ttgaactgca ctagcaccta atgcaaatga tgcagcaatt cctctaccat
     7741 ctacaattcc tccagcagct attacaggta tgtttacagc atcagcaact tgtggaacta
     7801 agaccatagt agtaagttct cctatatgtc ctccaccttc agtaccttct gctattactg
     7861 ctgtagctcc tagtttttcc attctttgcg ccaaagctat tgtaggtact acaggaataa
     7921 cctttgttcc agcttccttt aatctatcca tatactttgc aggatttcca gcaccagtag
     7981 taataacttg aactttttct tctataatta aatcaattat atcatcaaca aatggtgaca
     8041 taagcattac atttactcca aaaggcttat cagttaattt tttacactca acaatttctt
     8101 tctttatagc ttcttttggt gcgtttcctg ctgctattat gccaagccct cctgcattag
     8161 atacagcact tgctaatgat gcagtagcta cccatgccat tcccccttgg ataactgggt
     8221 attttatatt taatattttg caaattttat tcatacaatt ctccttcact tgaaatcata
     8281 aaagtaaact ttttattatt aatcaacttt attttgtgca taagtttaat tttgttaatt
     8341 aaagcatttt agatatttta tactataaag tccacttgac aactgaagcc ccccaagtta
     8401 gacctccacc aaagccaact aaaactacat tatcaccctt tttaatttta ccttctctat
     8461 aagcttcatc taacgctact gggatagatg cagcagacat atttccatat ttgtctatat
     8521 tcacataaac ttttttcata tcaagctcta atctcttaga ggaagcttgt atgattctta
     8581 tattagcctg atgtggcact aaatagtcta tatcagttgt atttaaattt gctttttcaa
     8641 gagctcttaa agaagtttct gccatctttc taactgcaaa tttaaataca tcactacctg
     8701 ccatttctat tgtatgtaga ttagcttcta cagtctcttt acttgcctta agtcttgaac
     8761 ctccagcagg tacttttaaa caatcttttc ctttaccatc agagcccata tgtgtagaca
     8821 gtattccacc ttcacttaca ggaccaataa cagctgctcc agctccatct ccaaataata
     8881 ctgctgttgt tctatcatca taatttaaaa acttagaaca agtttctgcc cctattacaa
     8941 gcacattttt atagcaacct gtttccacaa attgctttgc aatagttact ccatatacaa
     9001 atccagaaca agctgctgat atatcaaatg cagtagcatt tactgctcct attgcatctt
     9061 gtactataca tgcagttgaa ggcaaagaca tatcaggagt aattgttgct aatataatta
     9121 gctctatatc ctctacgttt aactttgcac attctatagc ttttaaggca gctttagatg
     9181 ctaagtcaga agttgcttca gactctttgg caaatcttct ttccttaata ccagtcctag
     9241 tacttatcca ctcatctgat gtatccatta ttttctcgaa gtgaaaatta tcatatgatt
     9301 gctcaggtag ataacttcct actcctaata ttccagcctt tgtattcatt atatattaaa
     9361 cctccaaaac gagtttattc atcttcgtta tttttatttt ctttattgta tttgcttata
     9421 aattgcttaa tatcttcaac gacttttccc tttgaaaatt ttattccttg atttatagca
     9481 ttttttatag ctttagcatc agaactacca tgagctttta taacgcctcc atttagacct
     9541 aaaagtggag ctcccccata ctcagaataa tctataaaac tttttaattt tcttaaatca
     9601 tcctttataa gtaatgctcc tattttactt tttgtactgg ccataaaagt ttcctttata
     9661 agactcataa ctgaaagagc tactccttca gcagatttaa gtaatatatt acctgtaaat
     9721 ccatcacata tgattatatc tgtgtatgca tttataactt ctctagcttc tacattacct
     9781 ataaaattta aatctaattt tttaatttct tcatatgacc ttttgactaa atcattgcct
     9841 ttaccttctt ctagtccaac atttgctaat gctaccttag gattttcaag acctaaaact
     9901 tttcttgaat aaatattact catagctgca aattcaacta gattcttagg tttacaatct
     9961 gcatttgcgc cacagtctgc tataagtgtc attcctcgct ttacatttgg tattgctgaa
    10021 cataagcaag gtctatcaat tccttttatt ctaccaacaa caaataatcc accagcaagt
    10081 aaggctcctg tatttccagc tgatattata gcatctgcct ttccttcttt tacaagattt
    10141 aatgatacta ccatagacga atctttttta cttcttatag cctttactgg tttatcttca
    10201 ttttctataa tctcagtagt atgaactatc tctaatttat ttctatcaaa ttcataattt
    10261 gaaaattcct tttcaagtaa atctttatct cctgtgatta ttaaatcaac ttgatattct
    10321 tttatagcat ttactgcgcc ttctacatta gatttaggtg cattatctcc acccattcca
    10381 tcaattacta ttttcataat ttccctccta atctaattct tcaaagataa actttcctct
    10441 aaatatttgc tcttgggctt tattattgat aacaactttt acataatgct ttttatctat
    10501 atttctatac acttctgctt ttgctataag cctatcttgt tgttctatca acttaaaact
    10561 ctttacattt gcaactctca taataacctt aggtgcatct attatagcca ttgccaatga
    10621 atctgctaga gaaaaaatat acgtatcttt taaagtatta gtcttagagt aaatcatgtc
    10681 ctgtgtaact tctaacatag atatacctaa tcgccctaca gataaatcaa tgatttctcc
    10741 tgtaatttcc tttactccta aagtctttac ttttgaactc tgagtttctg ctattgattt
    10801 gactctttct cttagctctg gtatgtttag actcattcta tctaatctta tggtctgtat
    10861 acttacatca aacagacttg ataattcttc atcagtataa aaaggatctg tctttagcat
    10921 atctataagc tctttttgtc tttgggcttt acttttcttt ttcatgttcc ctccaaattt
    10981 tatagctgta attagtacca gctactaata gtagtatata gtaacacaca aaaattttca
    11041 atacttatat tattattttt ctatttttta atatgcataa aatccactta ttttctctag
    11101 taaatcatct tcttttttta tatgtacaaa aaaaacatgt agagtatact ctacatgctt
    11161 tttagaagct attacttaga cacaacttct ttacctttat aatatccaca gtctggacac
    11221 actctatgtg gtaattttgg ctcatgacat tgtgggcaac ttacaaatcc tgttgcttcc
    11281 atctttgagt tagctgctct tctcattttt gtgtttgatt tagacgtttt acgctttggt
    11341 actgccattt acgccacctc cttagtcaaa atttaattaa ataaatcttt taatttagca
    11401 aaacgaggat ctatgtattc ctcatcgttt gtaatttcac tgcaagaaca ctcttcttca
    11461 tttaagtttg ccccgcatac ttgacaaaga cctttacagt tttcactaca aagaacttta
    11521 tgaggtacat taaaatctaa tgtctgttct attatatcaa taaggtcgat ttcttcacca
    11581 tcgaatataa aaggatcaaa ttcctcaaac tcatcttcat cataatcttc ttcttttact
    11641 aaaaaacctt gtatagaata ctctagtggt atttctactt ctttaagaca tcttgaacaa
    11701 ttgtcaacaa ttgtaaaatt gacatctata tctagataca aacctttatt agtctttgac
    11761 actttccctt ttaaatttac aggtgatact aatttgtaaa tctcatcaca ataactaata
    11821 gtatcaattt tttcacaaaa attcaagtct attttgtcag tttcttttct atttattttt
    11881 tctatgctaa ctttcattaa gttcacctca acatatcacc aaatttatta tacaaatcta
    11941 gcatttgttt gtcaagtatt tttacttgat aggcataggt ttaatttaat tattttacca
    12001 atcttaatgt atctctagct atcattaatt cttcattagt tggtattaaa agcattttta
    12061 ctttagaatc agcagtagat attactgttt cctttcctct aacatcatta gcttctttat
    12121 ctaatttcat tcctaaaaac tccatgttag aagcaatatc tgctcttata gcttttccat
    12181 tttcaccaac gcctgcagta aatactacga catccaatcc attcatttca gcagcataag
    12241 ctcctatata tttttgtact tttttaacat aagcttctaa tgtcgcttga gctctttcgt
    12301 ctccattttt agctgcatct tctatatctc tgaagtcact agaaattcca gtcattccat
    12361 atactccaga ttctttattc attaatttat ccaatccatc agcatctaaa ccttcttttc
    12421 tcattaagaa aggtaatata gctgggtcta tatctccaca tctagttccc attattaacc
    12481 cttctaatgg agtgaatccc atagatgtat ctacacattt tccaccatca acagcagcta
    12541 tagaagcacc atttcctaag tggcaagtta ctatttttaa atcttttata tcttttccta
    12601 atatctctgc tgctctttga gatacataat tgtgagaagt tccatggaat ccatatcttc
    12661 ttaccccata tttagtatat aactcatgag gtaaaccata taagtatgaa gatttaggca
    12721 ttgtttgatg gaaagcagta tcaaatacag ctaccattgg tacatctgga agtatagctt
    12781 tacaagcatc tattcccatt atgtttgctg gattatgaag tggagctaaa tctatacatt
    12841 cttttaaagc atcttctact tctggagtta ttactactga agttgcaaat ttttctccag
    12901 catgtacaac tctatgtcct actgcatcta tttctttcat ctctttaact gcaccaaatt
    12961 ccggatgagc aacagcctct aaaactaatg ctattgcatc tttgtggtcc ttcattggct
    13021 gttcaacaac gtatttatca tctctacctg ctttttcatg ttttaatata gaaccttcta
    13081 ttcctattct ctcaaccaat cctatacata aaacttcttc attattcata tctattaatt
    13141 gatattttaa tgaagaactc ccacagttta atactaatat tttcattatt atttcctcct
    13201 tagatttttt tataaataat atgtaataaa ttttgatttg taataaatgt ctaaataaaa
    13261 ctctatcttt tattatttt
//
LOCUS       DAMJVM010000095        13191 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.099, whole genome shotgun sequence.
ACCESSION   DAMJVM010000095 DAMJVM010000000
VERSION     DAMJVM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13191
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.099"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            268..1731
                     /locus_tag="PC523_002855"
     CDS             268..1731
                     /locus_tag="PC523_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDF2476360.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAVSWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTTLPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKYEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKENKHELN"
     gene            1787..2923
                     /locus_tag="PC523_002856"
     CDS             1787..2923
                     /locus_tag="PC523_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2476361.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKIAEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLIDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVEIFTKVMEEFGIYEDEQVLLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINPYAKDRVIDGIKILALTGFNLFENSELLKKMKEE"
     gene            2948..4366
                     /locus_tag="PC523_002857"
     CDS             2948..4366
                     /locus_tag="PC523_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDF2476362.1"
                     /translation="MTNKTEGKKKKLTFPDAYVLMIGLIVFAAIMTYIVPSGTYSRIE
                     DLNTGNMLVQAGSFKYGEQHPVSIMDVFRAIPSGLGKNSVTIFFVLIIGGAFGIVNFT
                     GSLDVLIGRVISKSQGSKKGEIIITSLVLTFGLAGGVVGMYEECIAFLPILMTLCIAL
                     GYDALVAAGILLLGLASGYGSATINPFTVGLAQGISGLPLFSGMWFRWIFWICTMILL
                     SGYIIFYCKKIKKDPSKSLVSDIDYSHWQLDESSIKEKLSSGNIRVLVTFFGGILVLM
                     IIIVKFSLGMSDLSAYFFALGILCGVVYGMSPNKIASAFVKGMQEMVYPAILIGFASS
                     IVVILEQGVILDSIVHGLSLVLNYVPRIFSGGVMMIMQSIINLFIPSGTGQAMVTMPL
                     MAPLADVIGVPRQVAVLAYQMGDGFANGIVPTLGVLMAGISIAHIPYTRWFKFASKII
                     LMELVLGFIFLMISVQINLGPF"
     gene            4578..5696
                     /locus_tag="PC523_002858"
     CDS             4578..5696
                     /locus_tag="PC523_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDF2476363.1"
                     /translation="METLKIRNIELSGTNYEIGYELGKLIANTPKILEGQINKSNVVT
                     KEEEKEMLELFDKYCPGLNEELKGFADAVQVNCNQILYYTMTYLKPGCSQVALAPELT
                     TNGHVLFARNFDFSHNMEDFVLCKTKVKGKYAHIGTTVMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSVGLRKPAVVGLQFWAVIRYLLENCKDVDEALEYIEDMPIAYNINLLLADK
                     SGNIALVETLDGKKGVNRINRLEKQREHFLHSTNHIHIDELHKLEPQSIKNSIHRYKL
                     IKEYINNSKKVGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNTGKFNILWGG
                     LESNNWESYYLKDDVKATTHLININIEKAPSDFFDFVE"
     gene            5847..6716
                     /locus_tag="PC523_002859"
     CDS             5847..6716
                     /locus_tag="PC523_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2476364.1"
                     /translation="MDYLEYFEKAVIYIENNLQENITVNDVAKESGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVNSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGNKKKLELDFMRHLIGNITIKPVIKEIDEIKIIGIKDKVILENNSLPE
                     LWDKFRKVHQIVPNTLPSERVFGICEATSEIHLISESMEFNEIIGVEVSSYESIPNSF
                     VSKTITEGKYAVFTHTGSLENLDKTYEYIWGTWFLNSKEEFDTRDDFEVYDERFLGPN
                     NINSQIDIYIPIK"
     gene            7091..7636
                     /locus_tag="PC523_002860"
     CDS             7091..7636
                     /locus_tag="PC523_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDF2476365.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNKVADKA"
     gene            7649..8662
                     /locus_tag="PC523_002861"
     CDS             7649..8662
                     /locus_tag="PC523_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDF2476366.1"
                     /translation="MDENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEVVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVSPNCISVVNGEDMQKKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     AKVISPLAGNIGGLLIISIICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPISVIIAYGVAVF
                     ALR"
     gene            8884..10857
                     /locus_tag="PC523_002862"
     CDS             8884..10857
                     /locus_tag="PC523_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2476367.1"
                     /translation="MSVTEKIPSIDHKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAIIKYIKGQGYKLEIKDEQILNKIIDINRKNVFSLNSKEDRVEF
                     ILNYLTELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFNNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKETKKVKDYDVKFDLLKSYDEYFIAREIKHIISEMFECILNDEE
                     IIYLTIPLVSGNAPASECALNSSRINKNIDELMESIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILDSLEKMMDNLMDLGCIDDKFRKNIFNREEKSPTVFD
                     KGLLFPHSVNEKSDKFLMAVGILEEPIKYANRNIKIILMTMFPCENKMDSDLLVKIYE
                     EILKIGQDVKLTNKISKCRSFLEFKKVLLKNLM"
     gene            10927..11286
                     /locus_tag="PC523_002863"
     CDS             10927..11286
                     /locus_tag="PC523_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476368.1"
                     /translation="MKVLVILFAAYILNTILIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSNGFIKFGEILKGRTVMAKFKEIEDIEGLDIYSAEQKFKNEES
                     IVQAIKFIKEKINVSFN"
     gene            11321..11677
                     /locus_tag="PC523_002864"
     CDS             11321..11677
                     /locus_tag="PC523_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDF2476369.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     ISTLRSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKNEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            11714..13006
                     /locus_tag="PC523_002865"
     CDS             11714..13006
                     /locus_tag="PC523_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDF2476370.1"
                     /translation="MLDLNRKLAKLEEEGKKIRVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNENDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAEIKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLEHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEYMDGYGAYTCYGTIEKYDVAKAMKAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
BASE COUNT         5029 a         1322 c         2502 g         4338 t
ORIGIN      
        1 tttcaggctt ttcattatat gtattccaat ttatattgtt tgattattgt tcaagcacac
       61 atcgttaaaa attttataaa tttatgttta gatatgatta atactcttga ttttagaaat
      121 aaatatttgt agattgtaga ttaaaaataa tataaataaa tagaaggtag attttttaga
      181 tatattaaga tgaagattat ataaaatatt gaaatatagc tgtttaaatt attgtctata
      241 aatataaaat gaatggtggt gagttacgtg accgatttag ttaacaccat taatggaatt
      301 gtttggtcac ctgtattagt tgcaatgtgt ttgtgtatag gattatattt ttcgttgaaa
      361 ttaaaatttt ttcaaataag agatgtaaaa gaaatgttta ctctcttgct tgaaggagat
      421 agttctcatg ttgggatatc atcatttcaa ggctttgcta catctatggc tggaagaata
      481 gggactggga atatagctgg tgtagctgta gcaatagcaa tgggaggtcc aggagcatta
      541 gtatggatgt gtataatggc tacattaggt tcagcaactg catttgtaga atctactcta
      601 gctcaaatat ataaagatga gcatgatggg cagtatagag gaggacctcc atattatttt
      661 gaaaaaggtc ttgggtggaa aaagtgttca gttttatttt caatagttgc aataataagt
      721 tatacatttt ttatgcctgg tacacaatca aatactttat atctagcaat gaatcaagca
      781 tttggatttt caaaaatgac aatagcagtc gcatcttcta ttgcattagc aataataatt
      841 tttggaggaa ttaaaaggat tggtttattt gctgaaaaag tagtacctat aatgggtgga
      901 gcatacctta ttatgacatt agtaataatt ataacaaata ttagtttagt tccagaagct
      961 attgtaacaa tgtttaaatc tgcatttgga tttggttcag tttttggagg aatgttaggt
     1021 tcagcagtat cttggggagt aaaaagaggt atatactcaa atgaagcagg agaaggttct
     1081 gggacttatg gtgcagcagc ggcagaagta agtcatccag ccaaacaagg attagttcaa
     1141 gcattttctg tttatgtaga tacattatta atatgtatgg ctacaggtat catgattgta
     1201 ataacaggaa tgtataatgt tacaggtgtt tgcacaacac tgccaggtgt agaacctggg
     1261 ccacaatatg tacagagtgc aataaataca ttacatccac aattaggtga tatttttata
     1321 gcaatagttt tattattgtt tacatttaca actttattag ccttttcata tatgatggag
     1381 actaatgtat cttacttagt aactagaatt aaagcagaaa aatatgaaaa gtacataatt
     1441 aacgtctgta gatttatgct tatagcatgt gcagcaatgt gttgttttat agaaccactt
     1501 acaagttggg cattaggtga tatatgtata ggtgtattaa catatataaa tttaattgcc
     1561 atctttacac taaaaaaacc agctataaag gcatattatg attatataag acagaaaaag
     1621 atgggaataa aaaggagcga tagaactttt aatcctatag aattagatat aagaaatgct
     1681 gagttttggg aaaatagata cttaaaagag aataagcatg agttaaatta aagttataat
     1741 aaaatataag aattactaaa taaacttagt gggaggtaag attggtatga aagaaagaat
     1801 aatacaagtg gcggattcaa agaaagaaaa aattctaggg ctatgtcaat ctttatatga
     1861 tgaaccagaa attgctttgc aagaatataa atctgctaaa aaaatagcag aatttctaag
     1921 agaagagggc tttgatgttg aggaaaactt ggcaggtatg gctacagctt ttaaagcaac
     1981 caaaaaaaat ggagatggtc ccaaaatagc atttatagca gaatatgatg ctcttccagg
     2041 aaatggccat gcatgtggac atcatttaat tgcatctatg ggagttggag ctggaatagc
     2101 tctttcaagt atactggata cttataaggg agaagtcagt ataattggaa cacctgcgga
     2161 agaaactgga gatggaaagc cttacttgat agaacatggt gtatttgatg gctatgatgc
     2221 agcaatgatg atacatccaa attcgaaaac ttgtgtgaca ccagaaatta tagcaatagg
     2281 aggattggac tttatattta ctggaaaagc atctcatgca ggagctaaac catataatgg
     2341 aataaatgca ctagatgcag tagtactact atataataat ataaatgcac taagacaaca
     2401 acttatagat ggaacgagaa tacatggtat tatactagaa gcaggaactg ctgcaaatgt
     2461 aatacctgat atgggaaaag taagattaga gataagagca aaagaacaaa attactttga
     2521 tgaagtagta gaaaaagtga aaaattgtgc tagaggtgct gcaatagcta ctggatgtga
     2581 acttgaattt tatcactttg aaccaacttg tcaaggatta aatgaaaata aagttttagt
     2641 agagatattt acaaaagtca tggaggaatt tggaatatat gaagatgaac aagtattgct
     2701 aggttcaaca gacatgggaa atttaagtca aataatgcca tgtatacatc ctttgatgaa
     2761 attttctgaa aatggagaag aattgcatac gaaagaattt ttagaagctt ccataaatcc
     2821 atatgcaaaa gatagagtaa ttgatggtat aaaaatattg gctttgacag gatttaattt
     2881 atttgaaaat tcagagttat tgaaaaaaat gaaagaggag tagactttaa acaaagggga
     2941 cttgactatg actaataaaa ctgaaggtaa aaagaaaaaa ttaacattcc cagatgcata
     3001 tgttttgatg ataggtctta ttgtatttgc agcgataatg acttatattg ttccctctgg
     3061 aacatatagt agaattgaag atttaaatac tggaaatatg cttgttcaag ctggaagctt
     3121 taaatatgga gagcaacatc cagttagcat aatggatgta tttagagcta taccaagtgg
     3181 acttggaaaa aattctgtaa caattttctt tgtacttatt attggaggag catttggtat
     3241 agtaaatttt actggttcac tagatgtttt aattggcaga gttatatcta aaagtcaagg
     3301 cagtaaaaaa ggtgagatta taattacaag tttagtatta acttttggct tagctggtgg
     3361 agttgtaggt atgtatgaag aatgtattgc ttttttacca atattaatga ctctgtgcat
     3421 tgctttagga tatgatgctt tagttgcagc tggtatttta ttgttaggac ttgcatcagg
     3481 ttatggttca gctactatta atccatttac agtagggcta gcacaaggta tttctgggtt
     3541 accacttttt tcaggcatgt ggtttagatg gatattttgg atttgtacta tgattttact
     3601 ttcaggatac ataatttttt attgtaaaaa gataaaaaaa gacccgagca aaagcttagt
     3661 ttcagatatt gattatagtc attggcaatt agatgagtct tctataaaag aaaaattatc
     3721 atctggtaac atcagagtat tagttacatt ctttggtggg atacttgttt tgatgataat
     3781 tattgtaaaa ttttctttag gtatgtcaga tttatcagct tacttctttg ctttaggaat
     3841 tttatgtggg gtagtttatg gaatgagtcc aaataaaatt gcttctgcat ttgtaaaagg
     3901 tatgcaagag atggtttatc cagccattct gatagggttt gcatctagca tagtagtaat
     3961 actagaacaa ggagtaattt tggattcaat agtacatgga ttatcattag ttttaaatta
     4021 tgttcctaga attttcagtg gtggagtaat gatgattatg caatccataa ttaatttatt
     4081 tataccttca ggcactggtc aagctatggt aaccatgcca cttatggctc cacttgcaga
     4141 tgttataggt gtaccaagac aagttgcagt tttagcttat caaatggggg atggttttgc
     4201 aaatggaata gttccaacac ttggagttct tatggctggt atatctattg ctcatatacc
     4261 ttacacaaga tggtttaaat ttgcatctaa gataatactt atggagttgg ttttagggtt
     4321 tatattttta atgatttcag tacagattaa tttaggtcct ttttaatgta tttacgtaat
     4381 atgtttatag aattttaagg taaattatat tgaggttaaa tattttgaat tataatatgt
     4441 taggtttaat tatggttttt gaaataaagg gtgaactttg tgaagttcac tcttttaatt
     4501 tgtagcacaa aatatcatta atgattacag ctttggtgat aaaattaatt tcaagatatt
     4561 tataggaggg gagtaatatg gagactttaa aaataagaaa tattgaattg agtggtacaa
     4621 attatgagat aggatatgaa ttaggtaaat taattgccaa tactccaaag atactagaag
     4681 gacaaattaa taaatctaat gtagtaacaa aggaagaaga gaaagagatg ttagagttat
     4741 ttgataaata ttgtccaggt cttaacgaag aactgaaagg ttttgctgat gcagttcaag
     4801 tgaattgtaa tcaaattttg tattatacta tgacctatct taaacccggt tgcagtcaag
     4861 ttgcgttagc accagaatta actacaaatg ggcatgtact atttgcacgt aatttcgatt
     4921 tttcacacaa tatggaggac tttgttttat gtaaaacgaa agtaaaagga aaatatgcac
     4981 atataggcac tactgttatg caatttggaa gaggggaagg aatgaatgaa tgtggattag
     5041 gcgtgagtca gtcatcatgt ggtattcctg taggtaattc agttggttta agaaaaccag
     5101 ccgttgtagg attacagttt tgggctgtta ttagatattt attagagaat tgcaaagatg
     5161 tagatgaggc gttagaatat atagaagata tgccaattgc atataatata aatttgttac
     5221 ttgcagataa gtctggaaat attgcacttg tagagacttt agatggaaaa aaaggagtaa
     5281 atagaattaa tagactagaa aagcagaggg aacatttttt acactctact aaccatattc
     5341 atattgatga acttcataaa ttagaaccac aatcaattaa aaattctatt catagatata
     5401 aattaattaa agaatatata aacaattcta aaaaagttgg agagaaagag ttaatgaatt
     5461 tgttgtcctc aaagtatcca aatggtttat cttgtaatta ctataatgat ttttttggta
     5521 ctttaaaaag tatagttatg gatttaaata ctggtaaatt taatatttta tggggtggat
     5581 tagagagtaa taactgggag agttactatt taaaagacga cgttaaagct acaacacatc
     5641 tgataaatat aaacattgaa aaggcaccat ctgacttttt cgatttcgtt gaataaaaat
     5701 gtgtttaatt agtttaaata attatgctct ataaatcagt taaaagtttt tatttgtctt
     5761 attgaattgg ttataatatt ttagtatagt taaaaatagt gttactttaa atatttaaag
     5821 attttaaata gaagagaggt gccatattgg attacttaga gtattttgaa aaagctgtaa
     5881 tttatattga aaataattta caagaaaata taactgtaaa tgatgtggca aaagagtctg
     5941 gatattctta ttatcattta acacgcttat ttaagtctat gtttggtgag agtgtaggaa
     6001 gctacataaa aaaaagacga ttagtaaata gcacaaaaga acttttatac agtgataaaa
     6061 aagtgataga tatagctatt ggtagcgggt ttgaatcttc agaagcattt agtagagctt
     6121 ttaaatctat atacaaagtt agccctatag agtatagaaa aaatagaatt gatgtatttg
     6181 ttggtaacaa gaagaaatta gaattagact ttatgagaca tcttatcgga aatataacga
     6241 ttaaaccagt tataaaagaa atagatgaaa taaagataat aggtataaaa gataaagtga
     6301 tactagaaaa taattcatta cctgaactat gggataagtt tagaaaggta caccaaattg
     6361 ttccaaatac tttaccatca gagagagttt ttggaatctg tgaagcaaca tctgaaatac
     6421 acctaattag tgaaagtatg gagtttaatg aaataatagg ggttgaagtt agtagttatg
     6481 aatcgattcc aaattcattt gtttcaaaaa ctattacaga aggaaagtat gctgtattta
     6541 ctcatactgg tagcttagag aatttagata aaacctatga atatatatgg ggtacttggt
     6601 ttttaaactc aaaagaagag tttgatacaa gagatgattt tgaagtttat gatgaacgat
     6661 ttttaggtcc taataatata aattcacaaa ttgatattta tatacctata aaataatcta
     6721 taagaaacta aaaaataatt tgattaaaaa atagatgctt gaaatagcat ctattttttt
     6781 atttttatca agtttaaata attttaacaa taaaagaaat agaaagaagt tcttaaattt
     6841 ctaataagaa tttagaatat agtcgaaata aaaattatat atttctgaaa attctaatta
     6901 gtacaatttt ttccgttgta aaattgaaaa aagatgtatt tatttatcaa aaatattata
     6961 ttaaaatgat attaataaaa aaaacattta taataatagc gtatagtaaa gaaaaaagaa
     7021 ttaatttgta aaatttatgg tgagtagtaa aattaaaaat ttaaaaacgt tatagggagg
     7081 ttataatttt atggaaaacg ttattacagg tctaagtaaa ggtgcagagt ggtttatagg
     7141 tttgtttcag aaagggggag aacaatttat aggtcttgtt tcagggactc ttccaacact
     7201 aattgtatta atggtagcta ttaactcttt gattaagata attggtgaag aaagagtcaa
     7261 taattgggct tctaagctag gaaaaaattc ttttacaaga tatattttac ttccgctagt
     7321 atcagtattt ttcttgggaa atcctatgtg ttatacattt ggtagatttt taaaagaaga
     7381 acacaaagca ggtttttatg atgcagcagt ttcatttgta catccaatta cagggctttt
     7441 tccacatgca aatgcaggag aactttttgt atggttagga atatcagcgg gtttgactac
     7501 tttaggtaaa gaaactacta cactggcatt atggtatttt atagttggtc taattgtaat
     7561 ttttataaga ggtatagtaa cagaaaaaat gtatgctttt ttaacaagaa aaaataaagt
     7621 agcagataag gcttaggagg tagataaaat ggatgaaaat aaaatattaa aaatagaaaa
     7681 aggaacttca ggttggggag gtcctttata tataaaaaaa gaaggcaata gaaataagat
     7741 attatcaatg actgctggtg gtatccatga agtaacatta aaaataaaag aactactggg
     7801 ttgtgaagtt gtagatggat ttaaaactgg agtttctgat gaagaagtgg cagtggttat
     7861 tattgattgt ggtggaactg ctagatgtgg agtttatcca aagaaaaaaa taccgacaat
     7921 caatgtaaat ccagttggaa aaacaggtcc tttagcaaaa tttataacag aggagtatta
     7981 tgtatcagat gttagtccta actgtataag tgttgtaaat ggagaagata tgcaaaaaaa
     8041 atctcaagaa aataaaagtg aaaataagag ttctattaga aagcctgata attatgatga
     8101 ggttaagtca aaagcacaag gagaatatgc taaaaagaat ataatacttt ctattggtca
     8161 aggagcagga caagtagtat caaaatttta tgatgcaggt cgagatacta ttcaaatggt
     8221 aatgaataat gtaataccat ttatggcatt tgtaagcatg cttatgggaa ttattttagc
     8281 atctggattg ggagattgga ttgcaaaagt catatctcca cttgcaggta atatcggtgg
     8341 attattgatt atatcaataa tttgtacact tccattctta tcaccaatat tagggcctgg
     8401 agcagttata gcacaagtgg taggaactct tgtaggtaca cagattggtc ttggagcaat
     8461 accagcatat ttagcgcttc cagctttatt tgcaataaat ggtcaagctg gatgtgattt
     8521 tgtcccagta ggattgagtt taggagaagc agaaccagaa actgtagagt atggtgttcc
     8581 agccctattt tactctcgtt taataactgg accaatatct gttattattg catatggagt
     8641 tgcagtattt gcattgagat aatatttaat gtcaatataa ggcttggaat taattactaa
     8701 aattgtaaat ataaatttaa gctagtcaag attttttgtt aatgaaagac tagcttttat
     8761 actattaatt agctgtaatt atcgagttag attaatacta aattttcctt tattaaagtg
     8821 aaattttatg tattaatttg gtttcaagtt tgtactagaa tgaaatcaag gaggagatac
     8881 aaaatgagtg taactgagaa aattccttct attgaccaca agatatttaa gatattgatt
     8941 atgtgcagta aaaaagagtt tgtgagtatt aattcaatag caaatgaatt gaatgtcaca
     9001 actagaagtg taaggacgta tataaaacaa ttgaataaag atttaggtaa tgatatagca
     9061 ataattaaat atataaaggg tcaaggctat aaactggaaa taaaagatga gcagatactc
     9121 aataagatta ttgatataaa tagaaaaaat gtattttcat taaattcaaa agaagataga
     9181 gtagagttta tactaaatta cttgacagaa ctagatggat ttattacact tgatagttta
     9241 gctgatgaga tgtgtgtagg aagaactact ttagtaaatg attttcagta tgtagaaaaa
     9301 gttttggctt catataattt aaacttaatt aaaaaacaga atacaggtat gaaattaaat
     9361 ggaaatgaac ttgatattag actgtttata ttaaatcagt tgtataaaaa ttcaaggaag
     9421 gatttcaata actcaaagta ttttaaagga atcaaaaaag aagaaatcat aaatttagaa
     9481 gaaaaactat tgactttatt taaaaaaaat aacttttatg ttacagatga aatgttaaga
     9541 gaggttatta actatatagt agttttagtc tatagagtaa aagaaacgaa gaaagtaaag
     9601 gactatgatg taaagtttga tttactcaaa tcgtatgatg agtactttat tgctagggaa
     9661 ataaaacata taatatcaga aatgtttgaa tgtattttaa atgatgaaga aattatttat
     9721 ttaaccatac cactagtgag tggaaatgca ccagcatcag aatgtgcttt aaatagtagt
     9781 agaattaata aaaatattga tgagttgatg gaaagtatat ttaatcaaat atatgttgat
     9841 atgggtattt ttataaatga ggatgaatta agagtaggat tgggatatca tcttagtttt
     9901 actttgaaca ggctattatt taatataaag ttaaaaaatg ttttgctaga agagattaag
     9961 caaaactata tacttccatt taaattagca caaatagcag gtaaagtaat agaaaaaaaa
    10021 tataatttag aagtttcaga agatgagatt gggtatatag caattcattt cagtggatat
    10081 ttagaaagaa atagtagtag attctattca ataaagaaaa tagcaattat atgtagtaca
    10141 ggtctaggaa cagcaaaact attaaaaata agggtagaaa aactgatagg aaataatcct
    10201 aaaattgaca ctctttcaag ctttaatttg agaaatatta atttagatga atacgatata
    10261 gtttttacaa cgattgattt agacacatct aatataaata caatagtatt aaaaataaat
    10321 acaatatttg atgaaaataa actaagagaa caattaaaaa cagtactatg cctaagagaa
    10381 ggaaatattg atactactaa ctctacaaat ttactaataa ataatttgtt agatgaagat
    10441 aaatttatga tattaaatga aaagactata cttgattcat tagaaaaaat gatggataat
    10501 cttatggatt tgggatgtat tgatgataag tttagaaaaa atatattcaa tagagaagaa
    10561 aaatctccta cagtatttga taaaggttta ttatttccac attctgtgaa tgaaaaatca
    10621 gataaatttt taatggcagt tggaatttta gaagagccaa taaaatatgc aaatagaaac
    10681 ataaaaataa ttcttatgac aatgtttcca tgtgaaaata agatggattc agacttattg
    10741 gtaaaaattt atgaagaaat tttaaagata gggcaagatg taaaattgac aaataaaata
    10801 agtaagtgta gaagtttttt agaatttaaa aaggtattac ttaaaaacct tatgtaacta
    10861 ctgtacagaa tctatagtta ttgtacagat taaaagaata atgtgatttt tggaaggtgt
    10921 ataaatatga aagtactagt gattttattt gctgcttata ttttgaatac catacttata
    10981 atgaagcaga ataagttgta ttttaaaact ttaaatagag ctaaaaaaaa gggtgatata
    11041 gtgtccacag gcaaaaagaa aagctatttt tctaaaggaa gtatagccat tatatcttca
    11101 gattcaaatg gatttataaa gtttggagag attttaaaag gtcgtacagt aatggctaag
    11161 tttaaagaga ttgaagacat agaaggatta gatatatata gtgcagagca aaaatttaaa
    11221 aatgaagaaa gtattgtaca agctataaaa tttataaaag aaaaaataaa tgtttctttt
    11281 aattaactca atataaatat aaattatcag gaggaatgtc atgaaatatg aagttaagat
    11341 aagtggaata ggaagtttag ttaaagagtt aatggatgaa agtaattgtc ttattatata
    11401 tgatgaaacc attaatgatg acgacttaaa ggatatatct gtaattcatt cgatatcgac
    11461 attaagaagt gatgttgaaa taggggatac acttactata ggaaatagag attattgtat
    11521 agtttcagtt ggtgatattg ctcaaaagac tcttagagaa attgggcatt gtacaatcaa
    11581 atttgatgga aaaaatgaag taaatttacc aggagaaatt catgtggaaa taggaaatcc
    11641 agatataact attggagatt taataactat ttcttaattt gaaaataaaa ttattataac
    11701 aggaggaatt ggtatgttag atttaaacag aaaacttgca aaattggaag aggaaggtaa
    11761 aaagataaga gtagctctta taggggctgg gcatatggga aatggaatgg taagccaaat
    11821 ggcaaatatg aaaggtattg aagcttctat tgttgtagat ataaatttgg agttggctca
    11881 taaagctttt actgatgcag gaatagatga agaaattata gacaatgtaa caaatgaaaa
    11941 tgatgcagaa ataaaattac aagaaggaaa agtattgaca tgtaatgatt ttcttgtagc
    12001 ttgtaaaact aagtcaatag atgtagttat agatgcaact gggggtatcg caattggtgc
    12061 agagatagca ttaaactcta ttttaaataa aaaacatata gttatgttaa atgctgaaac
    12121 tgattgtgtt gttggaccaa tacttaaaaa attggcagat gatgctggag taatatttac
    12181 aggttctgca ggagatgagc caggtgcagt aatggagtta tttgattttg cagatgcaat
    12241 gggatttgaa gttagagtga taggtaaagg taaaaataat aaattagatt tagattgtaa
    12301 ccctgataca gttagagaag aagcagaaat aaaaggagct tcacctcata tgatagcttc
    12361 ttttaaagaa ggaactaaaa caatggtaga aatggcttta atgtgtaatg caactggatt
    12421 tgtacctgat gtaagaggcg gccatggcat tgaagctact gttaatgaag tacctaagaa
    12481 atacgcatta aaaagcgaag gtggagtatt agataactat ggtgtagttg attttgtaaa
    12541 tggtatagca cctggagttt tcgtagtagt agcccataaa ttaaaagcag ttaatgatga
    12601 attaaaatat ttaagtatgg gtgatggacc taattacata ttatatagac catatcattt
    12661 atgtagctta gagactcctt tatcagcagc tatggcagtt ttagaacata aagctacaat
    12721 agttcctaaa gctggattag ttgcagaagt tatgactata gctaagaaag atttgaaaaa
    12781 aggtgagtat atggatggct atggagcata tacatgctat ggcactatag aaaaatacga
    12841 tgtagcaaag gctatgaaag ctgtacctat tggtttgata agtaaaaaga ctaaagttgt
    12901 caaagatatt aaaaaaggtg aagttataac ttacgatatg gtagagatag aaaaagatac
    12961 tactctatat catttaagac aattacaaga aaaaatattt gggtaagatt tgatagtaat
    13021 ataaatggat attaacaaaa agggttgatg attattttta aatcaaccct tttaaatttt
    13081 tgtatattct tgttaggata ctttctttag aattatatta taggttattt gtagttataa
    13141 aaattatttt taattagttc tgcaatttta tattcatatt aaaataatat t
//
LOCUS       DAMJVM010000096        13068 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.100, whole genome shotgun sequence.
ACCESSION   DAMJVM010000096 DAMJVM010000000
VERSION     DAMJVM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13068
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.100"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            161..1579
                     /locus_tag="PC523_002866"
     CDS             161..1579
                     /locus_tag="PC523_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDF2476371.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GIFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIVPMVIKQIVFGLAIGFVLAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYIGGNGYLSVYISGIIIGNSKIPHKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNIFGIAMVISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVIIENDIFHIIFFIALLSVGVQGTLIPAVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDDSSKWANKSIMDSNIPEDILIVMIKRKEQVLVPKGS
                     TVIKTGDTLVLSGDRIEELIQL"
     gene            1655..2467
                     /locus_tag="PC523_002867"
     CDS             1655..2467
                     /locus_tag="PC523_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2476372.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKEAIENPEEVENILKNKEKEMLDVIKSEEYKLIKLRN
                     YLVNLKGEVNMNDVIIKSLPKVKIASMRKVLKGYDELFNLFPNVMAEEMMKSECTCAV
                     PDYCFNIYHDGEYRETNMDVEMCQAINDLKEDTDILKFKELDEVESAVCILHNGSYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRIL"
     gene            2875..3492
                     /locus_tag="PC523_002868"
     CDS             2875..3492
                     /locus_tag="PC523_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDF2476373.1"
                     /translation="MEIFAIGHSNYPYDKLIDMIKKYDINCVVDIRETPYSKYNTQYN
                     KEAFNESLRNSGFVYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLKGIDRLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKILNKEGFDVKYIMHEGNLAYQQDIEESLLD
                     KYFADRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(3648..4100)
                     /locus_tag="PC523_002869"
     CDS             complement(3648..4100)
                     /locus_tag="PC523_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476374.1"
                     /translation="MVNLNENNLNVNMVYPISPTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTIGLGTMNIDKVYGQWAWFTDTIYDLNLFVFVGNYPYQIAKNRYDTFVNILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            4384..5742
                     /locus_tag="PC523_002870"
     CDS             4384..5742
                     /locus_tag="PC523_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2476375.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDNEKVNETIKQALVSGAILSFTITILM
                     WIFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIAIQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGVDLNPLGINLQTPSYGITGAAMSIAIARIIGCIVIVRAL
                     FKGNEFISIGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFVV
                     GAIFVPIAPWISSLYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     IA"
     gene            6015..6395
                     /locus_tag="PC523_002871"
     CDS             6015..6395
                     /locus_tag="PC523_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2476376.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRIFSFKDQDGNDYLVREDK"
     gene            6520..6720
                     /locus_tag="PC523_002872"
     CDS             6520..6720
                     /locus_tag="PC523_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476377.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            6916..7644
                     /locus_tag="PC523_002873"
     CDS             6916..7644
                     /locus_tag="PC523_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2476378.1"
                     /translation="MLEIKNLSFNVESNSEELGIINDVSLSFEKGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESDQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            7666..8583
                     /locus_tag="PC523_002874"
     CDS             7666..8583
                     /locus_tag="PC523_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HDF2476379.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTDTGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGKGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVVLEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            8720..9904
                     /locus_tag="PC523_002875"
     CDS             8720..9904
                     /locus_tag="PC523_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2476380.1"
                     /translation="MKNLLIRNGTIISPTDGIKFERRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPLNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESVVEKNI
                     EKIKGIKVKASKFQSNKNGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKKSGTLRKSVINAKDRGVLFDVCHGLDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHENKGRI
                     RIGGDGDFTIFTMEKSNDIIKDYNNNELILNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            10011..10199
                     /locus_tag="PC523_002876"
     CDS             10011..10199
                     /locus_tag="PC523_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDF2476381.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            10349..10999
                     /locus_tag="PC523_002877"
     CDS             10349..10999
                     /locus_tag="PC523_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2476382.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIEEDLENLE
                     QFIGPPLYDTFKEYYKFEDKKAKEAVEKYREYFTDKGIFENKIYENIKEILEMLYKKG
                     KILLVATSKPTVFAETILRYFDIDRYFKYVAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEELSESEPTYLVENVESIKDILL"
     gene            11102..12040
                     /locus_tag="PC523_002878"
     CDS             11102..12040
                     /locus_tag="PC523_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2476383.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYKGFK
                     KHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDSLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFVINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEVIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            12066..12917
                     /locus_tag="PC523_002879"
     CDS             12066..12917
                     /locus_tag="PC523_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2476384.1"
                     /translation="MNSKIKYFLKFFILIFILIFVYSVAIEPSLLIVKKYNISENGTL
                     IKNDIIESKSESIKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPNVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSDFNLLVNENKKIK
                     LKDNKYINILGVDEILNGHPNIKYLESQIDNKNFNLLLAHEPDLVDMLSKYTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
BASE COUNT         5215 a         1265 c         2314 g         4274 t
ORIGIN      
        1 taaatttaag tataacactc atattttaat gtattgtgaa atttttattg tactttatac
       61 actataactt ataaaaccta tgtcatatta tatttgaata agtaataaaa ttgtagatgt
      121 aaattaatct acaaaattta tatgaaatgg agttgatgct atgacagaat taatgattat
      181 aagtgggtta gtactattga tttgtataac ttctagcaag gtattgtata aatttggagt
      241 accaatatta ttgattttta tcttactagg gatgttattt gggtctgatg gaatggtagg
      301 aatctatttt gataactatg aattaacaaa acaattatgc tctattggac taatattcat
      361 aatgtttttt ggagggtttg gaacaaactg gaatatggct aaacctgttg ctataccttc
      421 tatattgatg tcttcactgg gtgtaataat tacggctggg gttacaggca tattttgcca
      481 tatggtaata ggaactagtt tacttgaagg tttattaatt ggggcaattg cagcttcaac
      541 agatgctgca tctgtgtttg ctgtgttgcg ttctcaaaaa ttaaacttaa agggttcact
      601 tgcctcatta ttagaagttg aaagtggaag taatgaccca atagcgtata tgttgacact
      661 aattcttttg acattgatga acaattctgg tgtagagctt attgtaccaa tggtaataaa
      721 acaaatagtt tttgggttag ctataggatt tgtactagca aaattatctg tttatatttt
      781 aaggcattca aacttcgaga tagaaggatt ttatactata tttataacag ctattgcaat
      841 actttcgtat gcatttagtg agtatatcgg aggaaatgga tatttaagtg tgtatatttc
      901 aggtattatt ataggaaata gcaagatacc tcataaaaaa agtttggttc atttctttga
      961 tggaatttca tggattatgc agataatgtt attctttatg ttaggattgt tatctttccc
     1021 ttccaagcta ccaaatatat ttggtatagc aatggtcata tctatattca tgatagttat
     1081 tgcaagacca ttagcaacat ttatagtact agcaaaattt aaattttcaa ataaagaaaa
     1141 actattcata tcatgggtag gtcttagagg tgcagcctca gtagtttttg caatttatgc
     1201 agttactcaa ggtgtaataa tagagaatga tatattccat ataatatttt ttatagcttt
     1261 actttcagta ggggtacaag gtacattgat accagcagta gctagaaagc tagatttagt
     1321 tgatgataat acacctgtat taaaaacatt taatgattat gatggagaaa ttagctctaa
     1381 acttatagaa gtaaatgttg atgatagtag taagtgggca aataagagta taatggattc
     1441 taatatacca gaagatatat taatagttat gataaaacga aaggaacagg ttttagtgcc
     1501 taaaggctca actgttataa aaacagggga tacgttggta ttaagtggag atagaattga
     1561 agaattaata caattataat ttatttcgta ttgactttaa cctaagggga aggtctaaac
     1621 ttaaattaat caaatctaat gcgcaggtga tgagatgttt tctataggaa tgttttctaa
     1681 aataaataaa ataactacaa agacattaag gtattatgaa gatatagggt tgttaaaacc
     1741 agagtatgta gatgagttta caaaatatag gtactatacg acagagcaat tacctaaatt
     1801 acatcagata ataactttaa aacaaatggg tttgtcatta gctgagataa aagaagctat
     1861 agaaaatcct gaagaggttg aaaatatttt aaagaataaa gaaaaagaaa tgttagatgt
     1921 aattaaaagt gaagaatata aactcattaa actgcgtaat taccttgtga atttgaaagg
     1981 agaagtcaat atgaatgatg taattattaa gtctttacca aaagttaaaa ttgcctctat
     2041 gagaaaggta ttaaaggggt atgatgaact ttttaatcta tttccaaatg taatggctga
     2101 agaaatgatg aagtcagagt gtacatgtgc agttccagat tattgtttca atatatatca
     2161 tgatggtgaa tacagagaga ccaatatgga tgttgagatg tgtcaagcta taaatgattt
     2221 aaaagaagat acagatatat taaaatttaa agaactagat gaagttgaaa gtgctgtttg
     2281 tatattacac aatggttcat attcaacatt gggaaaaact tacgcagatg catttaaatg
     2341 gatagaggat aataaatata aagtggttgg aaatcccaga gaatcttata tagatggtat
     2401 atggaataaa gagagtgaag aagattggtt gacagaagtt cagataccag tgaaacgcat
     2461 attataatat gtatacaaag tataaataag aataaaaaag tggtgtctca aaatgaattt
     2521 tttagtttat gaggcacctt ttttcatatg aaatcaagta tatttttata atttcaatga
     2581 tgaaaaaaag cttatctcta ttttgagaca gcctcttatt tttttattat aatagttttc
     2641 taaagatgtt ctatttatta aatttttata aataattata tttaatatac aaaatatgca
     2701 taaatcttga tgttttatat ataaagttgt atatttttta aggatataaa gtaaaaagtg
     2761 taaagtatca agaaaaaatc tattgggtat ttaaagtaat gctttattta attttaaata
     2821 aatcgggtat acatatatat aaatactaat tttaaagaat aggaggatat ttatatggaa
     2881 atattcgcaa taggacattc aaattatcca tatgataagc ttattgatat gataaaaaaa
     2941 tatgacataa attgtgtagt tgacattaga gaaactccat attctaaata taatactcaa
     3001 tacaataaag aggcattcaa cgaatctctt agaaattcag gatttgtata tatatacatg
     3061 ggaaaagaat ttggagctaa aagaactaat aaagatgtat atacacaaga aggttatgca
     3121 gattttgaga aagtagcaaa agaagatatt tttttaaaag gaatagatag attaaaaaaa
     3181 ggttgtcaaa tgggttatag gatagtttta ttaggagcta tgcaagaacc tataagatgc
     3241 catagaagta ttttggtagg aaaaatatta aataaagaag gttttgatgt aaaatatata
     3301 atgcatgaag gaaatttggc atatcagcaa gatatagagg aatcattatt ggataagtat
     3361 tttgctgata gaaagcaact gagtattgac aatttattag ggagtgcatt aacaagagaa
     3421 gaaatgatac aagaaggcta taaactggct aataaagaaa taggatatag aactgagaaa
     3481 ctaggtaaat aaaaatagat aagtagataa aacacagtac ttatcttaaa aaaataatat
     3541 tatgaattta taatagctaa gcatgatgtg ccaattaaaa atattctagt tatttaatag
     3601 tatcattaaa tttaggatat tttttaatag cacatcatgt tgtagtgtta aaaaataaaa
     3661 tctctaatat tgccataatc aactgttcta ttaagagtag gatgactaga tataaatcta
     3721 actgtaattt ttgaatttaa cagacaagga tttgaaatta aaaatactct atctccattg
     3781 acaatagaag taatagccac tggtaaaata tttacaaatg tatcatatct gtttttggca
     3841 atttgataag gataatttcc tacaaaaaca aatagattca aatcatatat agtatcagta
     3901 aaccaagccc actgaccata aactttgtcg atattcattg tgcctaaacc aatcgtatca
     3961 aagtatgttg ctatagttaa ataaatattt tccatgtttt ctaaatatga aacagtatat
     4021 cttctgaaac gtatgggtgc tgtgggagaa ataggataaa ccatatttac atttagatta
     4081 ttttcattta aattaaccaa agtatcacat cctaaaatta gtttataaaa tacaatattt
     4141 aaatggagac gattttgtta catctaaatg taatatttta aatgtggtag ccataaaaaa
     4201 taataaacat agaataatta taaagatata cggtgttcaa attatattaa acaagattta
     4261 tattatttga aagcaatata ttatgatgct aataatacaa ttttaaactt tgtattatca
     4321 atttacagtt cgataaacta attaaaaatt aaatttatat aaagagggaa aagaggaata
     4381 gggatgttta taaggaaaaa tgttataaag ttagcaattc ctataatgat agagcagacg
     4441 tttgtcatgc ttttaggggt atttaataca atgatggcag gtcatattgg ggaagaagct
     4501 gtatctgcag ttggaatggt tgattcaata aacaatatat ttatatctgt atttgctgca
     4561 ttatctgttg gagctactgt tgttgtagct caacaaatag ggaaaaaaga taatgaaaaa
     4621 gtgaatgaaa ctattaagca ggctctagtg tctggagcaa tattatcctt tacaataaca
     4681 atattaatgt ggatttttag aacaggtgta attaatttat tttatggttc agcagaagaa
     4741 ttggtaaaat caaatgcaaa attatacata gagtttacat tgtttacata cccattcatt
     4801 gcgatacaac aaatagcaaa tggtattcta aggggttgtg gagatgtaaa gaaacctatg
     4861 tatgtgacta tgtttatgaa tatagtaaat gtaacattag gatatatact tatatatggt
     4921 gttgatttaa atcctctagg aataaattta caaacaccat cctatgggat aacaggtgca
     4981 gctatgtcta ttgccatagc cagaataatt ggatgtatag ttattgtaag agccttattt
     5041 aaaggaaatg aatttatatc tataggtaaa atatttccat ttaagattga tgttgaaact
     5101 caaaagtcta tatttaatat agggatacca gctggagttg aacaactact ttttaatgca
     5161 ggtaaattga ttgttcaggt atttatagtt actatgggaa catcagcaat tgcatccaat
     5221 gcaataggaa tgtcaattgc atccataata aatgtaattg ggaatgctct agctttgtct
     5281 gccacaactt tggtgggaca gtatatagga cgtggagata ttaaaggagc taagagtact
     5341 ttattatatt taactaaatt tgcaacaatt tgtttatttg tagtaggagc tatatttgta
     5401 ccaattgcac cttggatttc aagtttgtat actcaaaatg ctagtgtaat tgacatttca
     5461 acacaattaa ttcgaagtga ttgtttagct atggtaatat ggcctatatc ctttgtatta
     5521 tcagcagggc taaagggggc tggtgatact agatatacaa tgatgactgc agtaataggt
     5581 atgtggattt ttagaatttt tacaggttac gtattggggg ttgtattaga atttggagtg
     5641 ttaggaattt ggataggtat gtatactgat tggcttgtaa gaggaacttt atattgttta
     5701 agattaagag gaacaaagtg gctaaaacac aaaattgcat agaatatatg aaatcacttt
     5761 tagatagaaa actctattta ggagtgattt tttatgtaat tattatcata attataagta
     5821 tgatatataa ggtgattaaa ttgcataaat aaagcctata ataatatgga tactataaat
     5881 tatatattga gcagaattac ataatttggt gataaatatt agaaatatat atctatagaa
     5941 aataatatat atgggtatat tattaaatat aaaaaatatt aggtatttat ttaataataa
     6001 aggtgggtat tgttatgata aataaaatag gaaaaataac tctctatgta aataatcaag
     6061 aggatgcaaa gttgttttgg acagaaaaat tgaactttgt ggtaacttat gaaaaggaaa
     6121 tgggtccaaa tatgaaatgg gtagaagttg caccaagtaa aaatgaattt acaacatttg
     6181 tgttgtatga taaaaatcta atgatgaagc aaaacaaaga gataaatgta tctcatccat
     6241 caataatttt aagtacaaga gacattaatg gaacttatga acaaatggca aataacgagg
     6301 ttgaggttgg agaattaatg gaaatgccat atggaaggat attttctttt aaagaccaag
     6361 atggaaatga ctatttggtc agagaggata aataatttta caaagtatta aatttactca
     6421 atatctgata taattagaga caataattat tataaactag tccagaatta gagttatata
     6481 attaaaaatt aagtgtaaat tttgagtaga ggtgataaaa tgtttaagaa aatggcagta
     6541 ctaaaagata tagcaactaa aataggtcgt aaaaaggctt atgaactatt agaaatggtt
     6601 gaaggtaatg atgcctttgt agctgaggta aagataaaaa agaatggaat agaatctaaa
     6661 aaagaagaaa ttatgttaaa agataatcaa aaaataatat tagagtatat agaaggttaa
     6721 tttttaaata tatataagat gatatactaa atttctaaga tgagtttgca taaaactcat
     6781 cttttttact tttcaataaa tttgttttat tatttaatac acattttatc tgcttagagt
     6841 tgctgatata taaaaattac tgatataata aaagaatgga tattaatgta atattttaca
     6901 gaaaagaggg gtattatgct tgaaataaaa aatctttcct ttaatgttga gtctaacagt
     6961 gaggaactag gaataataaa tgatgttagc ctttcatttg agaagggaaa acttattgtt
     7021 atcacagggc caaatggtgg aggaaaatct acaattgcta agcttattat gggaattgaa
     7081 aaagctacta gtggtcagat aattttagat ggagaagata taacaaatct tagtattaca
     7141 gaaagagcaa aaaaaggaat aggatatgca tttcaacagc caccaagaat aaaaggtatg
     7201 acagttgaaa atttacttac acttgctcat ggaaaaccat tgtctactga tgtatgttgc
     7261 caatacctta cagatgttgg attgtgttca aaagactatt taaatcgtga agttgataac
     7321 tctctttctg ggggagaaat gaaaagaatt gagatagcaa ctctttttgc tagagattta
     7381 aaggtttcta tatttgatga acctgaagca ggaatagacc tttggagctt tggcaaactc
     7441 aatgaaagct ttaagaaaat acatgaagag tctgaccaga ctataattat aatttctcac
     7501 caagaaagaa tattagagtt agctgatgaa ataattgttc tacaagatgg ttcagtaaaa
     7561 agccatggaa caaaagaggc tatattacct gaaatcatgt gtcaagttaa ttcaagctgt
     7621 gaattaatga aagatatgaa ttaattagaa aggaaggcaa attaaatgaa caaaatagat
     7681 ttaaatcttt tagaaaaagt tgctgaactt cgtggaactc cattaggagc atacaatata
     7741 cgtaaaaatg ggcaaggagt agagagacat tcaactgaaa atgtaactat aattcctaaa
     7801 actgataaac ctggaataga tattatagta aagccaaata caaaaggaga aaatgtacat
     7861 ataccagtaa tagtaacaga tactggtgta caggatatgg tatataatga ctttgaaatt
     7921 ggagaaaatg cagatgttgt tataattgct ggatgtggta tacataactg tggttgtgac
     7981 gatagtgaac ataatggtat acatagattt ttcttacgta aaggagctcg cctaaaatat
     8041 gtagaaaaac attatggtga aggtgatggt aaaggaaaga gaattttaaa tccagttact
     8101 attgttaata tggatgagga ttcatattgt gagatggaaa caattcaaat taagggtgta
     8161 gattcaacta aaagagaaat ggatgctaac ctaggtaaag gagctaaaat agttgtattt
     8221 gagaaattat taactcatgg taatcaagtt gcagaatcta atatgaaggt tgtcttagag
     8281 ggggaaaact cttctgctca agtagtttct cgtacagttg ggcaagataa ttctaagcaa
     8341 aaatttaatc cttgtgttat tggggattct ctttgttctg cacatgttca atgtgattca
     8401 ataattatgg gaaattctca aattagtgct atacctgaaa ttgctgcaaa tcacccagat
     8461 gctcttttag tacatgaagc agctattgga aagattgcag gagaccaaat actaaagatg
     8521 atgacattag gtcttactga agaagaagcc gagcaacaaa tacttaattg cttcttaaag
     8581 taaattaaag tttttattta atttaattaa tatagtataa tatagtgaat aagtctaaaa
     8641 tatttttatt tttaggctta ttttatttat gtaagaaaaa ctattagata aattatattg
     8701 taattaggat gtggaataaa tgaaaaattt acttataaga aatgggacaa taatatctcc
     8761 aacagatggt atcaaatttg aaagaagaga tatcctggtt ttacatggga aaatatatgc
     8821 tattggattg aaccttaaag aaaaaataag aaaagaaaaa ttacttacaa ataaagagtt
     8881 cgagataata aatgctgata atatgtatat ttccccagga tttatagatg ttcacaccca
     8941 ttgttataag agaaaattac ctacaggtat ggattctgat tcaattggaa ttgaaaaagg
     9001 ttctacagtt atatttgatg caggtacagc tggtccttta aactactatg attttaaaaa
     9061 aaagtatatg gatgagtgta aaacagaagt atattctttt ttaaatgtta caaatgaggg
     9121 actaaatata ttgaatgaat caacttcttt agatgttata aattttgaca aggttgagtc
     9181 agttgtagaa aaaaatatag aaaagataaa ggggataaaa gtaaaagcga gtaaatttca
     9241 atctaataaa aatggtatag atataattaa gaaatcaaag caagttgcta agaaattaaa
     9301 cttaccctta gtagtatata taggtgagta tccatcatat atagatgaag taatgaatat
     9361 tttgggaaaa ggagatgtgg ttacaccagt atatgggaat agtacaaatg gaatccttaa
     9421 aaaatctggt acattaagaa aatcagtaat aaacgcaaag gatagaggag tattgtttga
     9481 tgtatgtcat ggattagacc aatttgattt taaagtattt aaaaaagcaa taaagcaagg
     9541 tttagagcca gacctaataa gtactgatat gcatataaaa aacatgaaag acgtaaatta
     9601 ttctctttta aatgttataa ataaaattat ggaacttggc atatccttag aaaagtgcat
     9661 agaaaaagta actgaatatc caacaaaaat tttcgattta catgaaaata aaggaagaat
     9721 aaggattggt ggagacggtg attttacaat attcacaatg gaaaagagta atgatataat
     9781 aaaagactat aataataatg aactcatttt aaataaaaaa ttaagattac aatatactgt
     9841 aaaatcatgg atgaaaagca gtgaagttta tagacatgga tacgaaaata ctataataaa
     9901 ttaaacaata gtgataaaat aataaatata tgtttaaatt gtcatatatt ggataaatat
     9961 ctaaggaaac aacaattaaa aaataataat tttaggggag gtattttagt atgaaagcat
    10021 ttgttgataa ggatgtatgt gtagcttgtg gagcatgtat aggaacatgt ccagacgttt
    10081 ttagtatgag tgatgaaggg cattcagtag caattgaagg agaaatacca gcagacttac
    10141 aagatttatg taaagaagcc gaagaaggat gtccagtttc agctataaca gttaaataga
    10201 gttgtttaaa tttattttaa aaataaatat ataagattta attgagtact taagctatag
    10261 tattagaaat ttctattact atagcttttt aaaatagata aacttaaaat atagggtatt
    10321 attttttata agtatggagg actgaaatat gaagaaaaat tatgaaatag tattatttga
    10381 tttagatgga acacttacag accctaaaga aggaataaca aagtctattc agtattcttt
    10441 aaatagtttt ggtattgaag aagatttgga aaacttagaa caatttatag gaccaccatt
    10501 atacgatact tttaaagagt attataaatt tgaagataaa aaagctaaag aagctgttga
    10561 aaagtataga gaatatttta ctgataaagg gatatttgaa aataaaatct atgagaatat
    10621 aaaagagata ttagaaatgc tctataaaaa aggtaaaata ttattagtag caacttctaa
    10681 gccaacggtt tttgcagaaa ctatacttag atattttgat atagatagat attttaaata
    10741 tgtagcaggt agcaatcttg atggtacaag agtaaataaa aatgaagtta tacaatatgt
    10801 actagaccta tgtaatgtaa aagataaaga taaagttatt atggttggag atagaaagta
    10861 tgacataatt ggagctaaaa aaataggcat agattctatt ggagtgttgt atgggtatgg
    10921 ttcttttgaa gagcttagtg aaagtgaacc tacttatctt gtcgaaaatg tagagtctat
    10981 aaaagatatt ttgttataaa tatcttttat agacaaaaat attacaaaat ggtgtcgcat
    11041 atttaaaaat atgatattat agttaaggaa gtcatttaaa aaagaggagg atgcacttaa
    11101 aatgaaatct attttaatta tgggtggaag cgacttcata ggttctgcac ttgctaagcg
    11161 tttgattaaa tgtgggtatc aaattgatat acttacaaat ggtaaaaagg aaatagatta
    11221 taagggtttt aaaaaacatc taatttgtga tagaaaagtt cgaaaagata tggaaaacat
    11281 tatcacagga agaaaatatg attacatata tgatatgaca gcatatacaa aagaagatgt
    11341 aagtaatctg attgatttta taagtatgga tagcttaaaa aagtatatag tattgtcagc
    11401 aggagcagta tataaagata gtggaagaaa tataaaagaa gaaaatgaga aaggtgaaaa
    11461 tgaaaactgg ggaaaatatg ggttaaataa aaaagaggct gaagattttg ttataaatag
    11521 ccctatacca tatataatta ttagaccaac ttacatatat ggtgaaaata ataatttata
    11581 tagagagtat tatttctttg aaaaaataga aaaaaatgaa aagataccag taccaaaagg
    11641 aaaacaagtt agtaatcaat ttatatatat tggagatttg gtaaaggtat tagaaagtat
    11701 aatgaaaaat cctcatgtaa gagaggcata taatgtgacg aatccacagt taatttcttg
    11761 ggatgacctt atatatacgt gtggagaagt tattggcaaa gaacctataa taaaatatgt
    11821 agatatggaa aaagtagagt ttagagaaag aacgtatttc ccatttagaa atatagactt
    11881 taatcttgat ataaataagc ttattgaaca tgggctttat ataccaaatg tgttactaaa
    11941 agaaggtctt acagcaacat ataagtggtt ttcagcaaat aagccaaaaa tgcatgacag
    12001 aaaaatgaac aaagttgagt ctgtaatgca aatagtttaa aaaaataaat aagtaggtag
    12061 atattttgaa ctcaaaaatt aagtattttt taaaattttt tatcttaata tttattttga
    12121 tttttgtata ctcagttgca atagagccga gtctattaat tgtgaaaaaa tataatataa
    12181 gtgaaaatgg aacattgata aaaaatgata taattgagag taaatctgaa agtataaaag
    12241 tagttcaaat ttcagatact caaataggaa gtttttactc aactaagaat ctcaaaaaag
    12301 tggctaataa gattaatact ttgaatccag atataatagt atttactggt gatttgattg
    12361 attactccaa taaaaatcca aatgtagatg aaattacaac tattttatca agtatgaatg
    12421 ctaggttggg aaaattttca gtattcggta atcatgatta tatgtataaa cttcctaggt
    12481 attataggca gattatgaaa aattccgact ttaatctttt agtaaatgaa aataaaaaaa
    12541 taaaattaaa agataataag tatattaata tattaggtgt tgatgaaata ttaaatggac
    12601 atcctaatat aaaatatctg gagagtcaaa tagataataa gaattttaat ttattgttag
    12661 cacatgaacc agatttagta gacatgctta gtaaatatac aatgaattta gttttatctg
    12721 ggcatagtca tggtggtcaa ataagactac ctattaaggg agcattagtt actcctccat
    12781 atggaagaaa atatactaaa gggttttatg atataaatgg aaatcattta tatgtgagtt
    12841 caggtcttgg aagcacaaag ttaccattta gattttttaa tataccagag ataatagaat
    12901 ttaatattat aatctaacat aaaatattta gaaaatatca ttttgtttgt tataatatgt
    12961 aaatgtatat actgtaaaaa tagattaaac tagtagattg aatgaattaa tagattaaat
    13021 taatatataa aatatttaag ttataattaa tgtattagac aaaataaa
//
LOCUS       DAMJVM010000097        13015 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.101, whole genome shotgun sequence.
ACCESSION   DAMJVM010000097 DAMJVM010000000
VERSION     DAMJVM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13015
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.101"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(118..1425)
                     /gene="grdB"
                     /locus_tag="PC523_002880"
     CDS             complement(118..1425)
                     /gene="grdB"
                     /locus_tag="PC523_002880"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(376..378),aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HDF2476385.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEENPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVVTGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVNYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEAIEEQTVFE"
     gene            complement(1502..2788)
                     /locus_tag="PC523_002881"
     CDS             complement(1502..2788)
                     /locus_tag="PC523_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HDF2476386.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYINSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            complement(3067..4389)
                     /locus_tag="PC523_002882"
     CDS             complement(3067..4389)
                     /locus_tag="PC523_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF2476387.1"
                     /translation="MKILIVDDELEYGVVMKKILQKKGYLVDVTLSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKAINKDIEVILVTGYGSIENAVDAMKKGALSYFIK
                     SNPIENLLEEVEKVKTSKTSVSLQKNNLEFTLESKNRDFNDVIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLATLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIKP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKIAEYNIK"
     gene            complement(4389..6383)
                     /locus_tag="PC523_002883"
     CDS             complement(4389..6383)
                     /locus_tag="PC523_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2476388.1"
                     /translation="MNKKRIVIIGIIYSFLVIFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIVVDLINSLSIQIGRDFYFKP
                     NNWWKESFINSIDDSIKFFDLIPSKERASKFIFTDPIYTLSANILKDKKSEDINSYMD
                     LKGKTVAIPEGDYSINFLKQKVQDINILLTPDIKTGVNHLISGKVDAVVGDEPVLRYY
                     INNYGISNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEEVMENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDVMGEMSYKIADLIDMIKGVIENTFSENTN
                     KTSEIEVHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKILQLKNVEIKIDCEHNLVCYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDALRDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNREVTI"
     gene            complement(6470..7717)
                     /locus_tag="PC523_002884"
     CDS             complement(6470..7717)
                     /locus_tag="PC523_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2476389.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMATGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALLSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILFAMLGGGKAGNIISPNPNT
                     IAVSGNFSVNLSSVMIANIIPAIVGVIITVILASILINKGNKVQSYEILEKREDLPSL
                     FKSLCGPIIAIFLLFLGNVFPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIAALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIINSGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            7935..8480
                     /locus_tag="PC523_002885"
     CDS             7935..8480
                     /locus_tag="PC523_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2476390.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHNNRKKVILPGSKNVVYEILIPDSS
                     GNLEFAIMNLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLFKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            8494..9180
                     /locus_tag="PC523_002886"
     CDS             8494..9180
                     /locus_tag="PC523_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDF2476391.1"
                     /translation="MKTYKFIFFDADDTLFDFRKSESHAFKKLLSEFNLELNFENYIK
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKINLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILPYLKEKYTIVIITNGIYEVQKKRLENSRIKGYIDGIVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALMVGDSLTSDILGGINSGIDTCWLNSNNNINYTNHIPTYEI
                     NTLLELNKLL"
     gene            complement(9304..9924)
                     /locus_tag="PC523_002887"
     CDS             complement(9304..9924)
                     /locus_tag="PC523_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476392.1"
                     /translation="MNYNSNKDKFILADINLADIFIDKNIVLKFLGYGNRKAPDTISE
                     IVEREIRNINDILDIKIYISEVNIDKTPKECKKAFAVMYTIGDKIDIKMNDYMKNCNM
                     MAGLALDKIGIVCLDCINEKIKMYLKEKYDSLKISHEIYPGDKEFEVERQKDIYNYIK
                     NKYSSVEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(9914..10936)
                     /locus_tag="PC523_002888"
     CDS             complement(9914..10936)
                     /locus_tag="PC523_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HDF2476393.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDREKLRKL
                     DVPDPYTSGRMPLNIESLSLISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEYIVKNLNKIYDEL
                     DCWKGMHICGDTRELLDNMLDCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRGYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDINEL"
     gene            complement(10937..11575)
                     /locus_tag="PC523_002889"
     CDS             complement(10937..11575)
                     /locus_tag="PC523_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HDF2476394.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINILKKTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNRIKVDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            complement(11702..12853)
                     /locus_tag="PC523_002890"
     CDS             complement(11702..12853)
                     /locus_tag="PC523_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2476395.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIPYEIVEFDETNF
                     EKDDDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGTIAYNKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     IKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDSELLKYMEFKQVENKYIIKYKEEELV
                     IESLDKLNKLFFEKNEEQYNEFKHLKNIYKFATKAFPVDFPWTANLNYQ"
BASE COUNT         4199 a         2285 c         1305 g         5226 t
ORIGIN      
        1 atgtacaaat agtattcaat atttcgaatt tatctaacaa aaaaaggtat cgtaaaacaa
       61 ttttaaaaaa ctatttttcg ataccttttt taattttaat gttgtgttat atttttatta
      121 ttcaaatact gtttgctctt ctatagcttc tgttagtgca tttaaagctt ttaaaactat
      181 attctttcta atttttttac tttcctcttc tcctaacata ggatttccaa gtggatatgg
      241 tatccctact gctggaacaa ttctattagc tcctattgta agtgatattg gtactactgt
      301 acatatatga acaactgcta taccagctct ctcaatctct tttaccatcg ttgctccaca
      361 acgagtacaa gtacctcatg tagaagttaa aattacagca tctacatcat cttctaacaa
      421 tttcttagaa aactcttctc caaatctttt agaattacca acactcgttc ctgttcctgt
      481 agtagttata aaataattta ctaattctcc aattacacct tctttttcaa gttctctaac
      541 tacatctagt ggtacaacta aatttggatt ttcaagaaca tatgctctat catacccacc
      601 atgtatagtt gtaaattctt tatttgacat agaatccata tctgttaaat cataaattcc
      661 atatttagta gcacttgaag attcaatttt atctggatta tcaattggaa ctatacctcc
      721 agaagttact agtgcaagtt ttatcttaga taaatcttta acaggtttca taggtgtaac
      781 tctatcaaat tcaggcattg gatactcagt ttcaaagctt tcacctttca ttttcttaac
      841 taacatatca attgctcttt cagaccctct aagctcatgg aagaaattct ttcttattcc
      901 tctcattata tatccttctt cttcaggagg tcctatttcc tcacctctaa ccaatttaag
      961 acctagtttc gtcattattg gaactacctt tctaagactt gctgctgaat ttcctgtaga
     1021 tactatatgc aaatctaact taaacatatc tacacctgga ttttctttat acattcctgt
     1081 aactacagga atatttagct cttcttcaac tgccttacaa atagtcccac aagctactcc
     1141 atatctacct gcattaaatg ctggacctgc aataaataaa tcaggatttt cttcttttat
     1201 catttttaaa agttcttgag ttgcactctc aatattttca ccaaaatacg tatctccaca
     1261 aattacagtt gagattatct ctgcatcatc attaaattga ctctgaaatg ctattccagg
     1321 tcctactaca ccagcccttt tttctggcgg aatatctgct ttttcctctc cacctatacc
     1381 agcaaagaaa ttgtttatat aatgaaccac tctgatttta ctcatctttt aaccctccaa
     1441 atcactttat agttattaca tcaatgatta caccaacaga actttatatt caaatgctta
     1501 tttaataata tcttgctgtc aaatgattat agccatttgc tatagttgat gctattataa
     1561 tttgtaactc tgcatccatg cttccatctt catttaagca tcctttatac ccacctatca
     1621 tcatttcaac atagtctaaa gttcctataa ttttttccat tgctgggaaa tgaaccacta
     1681 gatttccttg accacaagaa acaactgcat ctgcttcctt agtagcatct gctatagatt
     1741 gactcttgcc atcttttcca ggaaattcat ctgttatcaa aactacattt gctccaactt
     1801 cactacattt tctacaattc atcattaggt cagtatctgg atttccataa ccttcttctg
     1861 tcactagaac tccatcaact cctaaaaatt ctattaattt agctaccata tcagagcttc
     1921 tctctttatc aactaaaaat acattctcat tagtcaaaat aactcctata aagtttaaat
     1981 cttttccatg tcttttatat aagtcatgta taactggatt atttaaatga tggaatgtcg
     2041 taaccttatc acatggtgct acacagtttc cagatattat agctccatcc attatttcag
     2101 ttggatacat aaatgtagga actatctgtt tagcatccac cccgtagtaa taagtatcat
     2161 gtaaaagtcc ttgtgattgt aacatatgaa catatgctac tgttggcagg tctggatatt
     2221 cttttacttg ttttaataat ggctttgtct catatacaat gacttcatct ggctcaactt
     2281 ctcttcctgc ttcgcctacg tatgcaccta tttttaatcc agcaattcta gctgcttctt
     2341 cataatcatg agtatctata ccatcctttg cttttataac aatacatata ttatttgttt
     2401 ttgaaaaagg tgtatattta gcagtaggac cactcatatc aattacgcct tcttgaaatc
     2461 caactatatt tccacaagtt acaacacaag ctcctaaaag tacatgggtt cttccacttc
     2521 ctacagtctt cactttattt ataattccag gaaatatttt gctttcatct ccaattttaa
     2581 ctctaggttc tattacatct ttaactggag ctattcttat tgattcgcca ggtcttgcaa
     2641 gttcaacatt tacacttaca atcctttcat cttctaatac tattttttct atttcttcgc
     2701 tattgatata tagtacccca tcttttactt ctgtcttttc tccaaattca acatttttta
     2761 taaatatatt ccctagctct agcttcatat gaaaatcccc cttaacatat ttaatcgcaa
     2821 ttaaaagtat ttattacgtt tattaatgta tgaagcattt tctatgccaa ctatttaaaa
     2881 tatatattct atgtattaat gtcatagaaa tttgcatttt taatttacat cagtattgaa
     2941 tttaccaata atcattgtaa atattaattt ttaaaattat aataaaaaaa gataaacaga
     3001 ttttttctgt ttatctttta gtacagtttg agaaattttt ttctcactta atgagaagtt
     3061 tttttctcac ttaatattat actctgctat tttattggtt aactgtcttc tactaatctc
     3121 caatttttta gctgtatttg aaatattatt tccacataaa gataaaactt tttcaatata
     3181 ctcacattca aactcttttc ttatttctct taaaggttta atacttattt tatcatctat
     3241 atacacatta tttgatatta aatttaaatt atcttttgat agattacctt cttcgcttaa
     3301 aactacaagt ctatttatta tattttttag ttctcttata tttcctggat aattgtaatt
     3361 taataaaaag tcttttactt ccttatcaat tgagtgtatc tttttatcat gttctatttg
     3421 atacttattt aagaaaaatt ctataagagt agctaagtct tctcttctct ttcttagtgg
     3481 tggtatagtt attgttatcg tacttattct ataaaaaaaa tcttctctta tatttccatt
     3541 tgatatttcc acttttggtt ccttattcat agcacaaatt aatctaaagt caactttaat
     3601 agatttattg cttcctatcc tctctataga tttgtcctca agtgtcctta aaagttttac
     3661 ctgaacattt aaaggtatat ctcctatttc atccaaaaat aaagttcctt tattcgaaag
     3721 ttcaaatcta ccttttctac tatctactgc tcctgtaaaa gaaccttttt catgaccaaa
     3781 taactcagac tccaatagat tttctgaaaa agagcaacaa tttacaggta caaatatctc
     3841 attctttctt ggacttatgc tatgtatata tctagctaat atatcttttc ctacaccaga
     3901 ttcacccaag attaatatat taacatcttt acaagcagct ttttttgcta tttttatgac
     3961 atcattaaaa tctctatttt tagattccaa agtaaactcc aaattatttt tttgtaaaga
     4021 aacagatgtt ttacttgtct ttactttttc aacttcttct aataaatttt caataggatt
     4081 actttttata aaataagata aagctccttt tttcatagca tcaactgcat tttcaatact
     4141 tccataccct gtaactaata taacctctat atctttatta atggctttaa ttctatctaa
     4201 tagctgtact ccatccatat ttttcatcat aacatcagat aaaaccaaat cataattttt
     4261 atctttctta attatattta tagcttcttc accacttagt gttacatcta caagatagcc
     4321 cttcttttgt agaatttttt tcattactac tccatactct agctcatcat caactattag
     4381 tattttcatt atatagtcac ctctctattt aatggaattt ttatatgaaa acaagttcca
     4441 acccccaatt tactttcaac acttatatcg ccattattct ttttaatctc attataagta
     4501 atgtataaac ctaaaccagt tccctcacca attggttttg tagtatagaa aggattaaat
     4561 atatccctta gagcatcttc ttttattcct tccccattat ctttaaagtc tataaataaa
     4621 caatgatttt tttcatagca atatataata atttttccat cttgatgtat tgcatctatt
     4681 gaattagaaa ttaaatttat aaatacatgt tttaatgatt ctccatttat atagcaaact
     4741 aaattatgtt cacaatcaat ttttatttca acattcttta actgtaatat tttatattga
     4801 agtttcacta tattttcaat aaaatttctt atatttatat gttctaaatt atcatctgat
     4861 attctcgcaa aatttaacaa attagttatt atgttacttg ccctatctac attactttct
     4921 attgatttaa cacaatcatt tacttcttcc attgtaacgt tatcttttaa gagatagcaa
     4981 ttatttctta ttatacctaa aggatttctt atctcatgtg ctaccccaga agctaattgt
     5041 cctatagata tcattttgtt ttctctaagt aatttcgcct ctactatttt atcattagtt
     5101 atatctttaa taagaactac tatgtactca gtattatttt ttttatactc tacaggaaaa
     5161 gtatttatct ttaaaatctt attgtgtact tctatttcac tagttttatt agtgttttca
     5221 gagaaggtat tttcaataac accttttatc atgtcaatca aatctgctat tttataactc
     5281 atttcaccca taacatcata tagtactttg ttcgattcta ctatattgtt attttcatct
     5341 acaagcatta ttatatctgt gatactatta aaagttgcct ctaaagtttt tttattttcc
     5401 attacttctt ctgtttttct ttttatttct atctttaaca aatatgtata gctataaaaa
     5461 acataaattg atattaaaat tatacctata aaaagataaa tactaggtac aattcctcgc
     5521 tcatacaata tatcatcaac ttcattatat gtagaatacc attttttctt taaatcttta
     5581 tacactccat ttttctgaag tttaaaaatg cctttattta aaatactgac cagctcttca
     5641 tattgtttag gtacagcaag aactgccttt ttagtatata taggattact tagaacactg
     5701 tatttatttg aaattccata attatttata taatatcgca atactggttc atcaccaaca
     5761 actgcgtcta cttttcccga tattaaatgg ttgactccag tctttatatc tggagtcaat
     5821 aaaatgttta tatcttgaac tttttgcttt aaaaaattta tagaataatc accttctgga
     5881 attgctactg tttttccctt taaatccatg taactattta tatcttcaga ttttttatct
     5941 tttaatatat ttgcactcaa agtgtatatt gggtcagtaa atataaattt acttgccctt
     6001 tcctttgatg gaatcaagtc aaaaaacttt atactatcat ctattgaatt tatgaaagat
     6061 tctttccacc aattattggg tttaaaataa aaatctctac caatttgaat tgacagagaa
     6121 tttattaaat caacaacaat tcccttatat tgtccatctt ctttaccctt atatctcaaa
     6181 ggaggtgaac tttggtcaga actataaatc aaattcttgt gtttttctaa ccactttttt
     6241 tcttcttctg taaacggaag tgatttctta atatattcaa atacatttag attatactcc
     6301 atatttacat acatatttgt aagtgaaaat atcactaaaa aggaataaat aatccctata
     6361 ataacaatcc tttttttatt cactttaaaa ctccaatctt cttttttact ataataaatt
     6421 caatatttaa taacaaaatc ctactttttt cattgaatat ttatatttat tataaaacta
     6481 attgaataga tgttgaaatt atagtcatta ctattcctat cagtgcttca tatggaatta
     6541 atttaaatct ttcacctata tccatattta tactacctgc actagtatga aatagtgagc
     6601 catgtggtaa ttgttcaaat acactagacc cagaatttat tattgatgca cctgaaattg
     6661 gtgataatcc accatttatt atagcatcat gaaaagtaga tgatgctata gtagcaccag
     6721 ctgttgatga tgctgttgct aacgacataa gtatacccga aactggtgcc aataaaaatt
     6781 gtggcatatt taaaaattgt agagcagcta ttgtgctctg ttgtaattca gacatctgta
     6841 taattcctgc aatagtccct gttccaagta ataatataca gaccccttgc atcttagata
     6901 atccaaaagc taaatattct cttgaattgt tcaaatttcc tgtgacaatt agagttacta
     6961 ttcctcctat tggaagagct atcatggggt ctataactat aggaaataca tttcctaaaa
     7021 acaatagaaa tatagctatg attggaccac ataatgattt aaataaactt ggtaagtcct
     7081 ctcttttctc taagatttca tatgattgta ctttattccc tttatttatt aatatacttg
     7141 ctaaaataac tgtaataata actccaacaa ttgctggaat tatatttgca atcataacac
     7201 tagagagatt gacagaaaaa ttacctgata ctgctatagt atttgggttt ggtgatatta
     7261 tattacctgc tttacctcct cccaacatgg caaatagtat tgacaattta gaataattta
     7321 attttctacc tatatataat cctataggtg ctacagttat tatagataca tccaaattta
     7381 ctcctactcc agcaagtacc atagttgaaa tggcaatcga aagtaaagcc ctcttttcac
     7441 caaatatttt tacaatctgt tctgatattt tatctactgc ccctgttttt attaaacttc
     7501 cagctaatac acctgatgcc aaagctctaa gaattgatgg tgatatattc atagctccag
     7561 ttgccataaa atctacagtt tgtgttatgt ttgctcctcc aactaatcct ccaataaatg
     7621 ctcctaacat taaactatat acagcttgaa atttttttat aattaaaata attgctatta
     7681 ataaaccaaa tatagctcca aaagtcgtta tttgcataat atcccctcct atcataaatt
     7741 aattatcttt tatttaaata tattttaagt attctgtatc cactttgtca attatatttt
     7801 aatttgttta attagattga acatagtttt ttcaaaacct cattctatat gttataataa
     7861 tttcatacta tataatcttt ttacttgatt attttaagtt aatatttttt aaattaaaaa
     7921 gagaggatat ttatatgaat gatttaaatt taggtgaaaa aatagctgaa gttcgaaaaa
     7981 aacaaaactt aagtattcga gatttagcaa aactagctga tgttacacca tcattattaa
     8041 gccaaataga acgaggtctt gctaatccat ctgtaaattc attaaaatcc atagcctctt
     8101 ctctaaacgt tcctttattt acattttttg tatctgaggt agataaaaaa aacttgatag
     8161 taagacataa taatagaaaa aaagttattt tacctggaag caaaaacgtt gtatatgaga
     8221 ttttaattcc agattctagt ggcaatttag agtttgcaat tatgaattta gctccaaata
     8281 cttcttcttg tgttgataga attactcata atggagatga aattgcatat gttttggagg
     8341 gagaggttaa acttttcatg gatgatgatg aatttacttt attcaaagga gatagtgtaa
     8401 aagttccttt aggaacaaag cataaatggc aaaatgactc aaatagtgaa agtaaagtaa
     8461 tttttgcagt tatcttgtaa aggagtaaaa accatgaaga catataaatt tatatttttt
     8521 gatgcagatg atactttatt tgattttagg aaaagtgaat cacatgcttt taaaaaactt
     8581 ctatctgagt ttaatttaga acttaatttt gagaattata taaaatcata tagaaatata
     8641 agtgataagc tttggcttga ccttgaaaaa aatattataa ctttgaatga acttaaatta
     8701 cttagatttg aactttttgc aaataaaata aatttggatg ttgattctga aacccttagc
     8761 aaaatgtact taaatttttt aggtgaatgt acatttttaa ttccaggtgc aatcgatata
     8821 ttgccatatc ttaaagaaaa atatactata gttataataa caaatggaat ttatgaggtt
     8881 caaaagaaaa gacttgaaaa ttcaagaata aaaggatata ttgatggaat agttgtatct
     8941 gaagaattaa aaatatcaaa accaaatcct gaaatattta aatacgctct caaaaagttt
     9001 aattgccatg ataaatcttc tgctttaatg gttggtgata gcttaacctc agacatctta
     9061 ggtggaataa attcaggaat agatacttgt tggttaaatt ccaacaacaa tataaattat
     9121 actaatcata taccaactta tgaaataaat acacttcttg aattaaataa actactatag
     9181 cttgtaaatc tgttcagatt aaacagaata ttattttgca ataatttttt tagtttatat
     9241 acaaaataac taaaaaaatt attgcaattc atacacagaa caaatctcag tcttgaaaac
     9301 tatctaaaaa cattttctag gacacttact acatcttgat tctaaatttt cttcatcccc
     9361 cattaaaata agcattgcaa cactttttat gggcgatagt tgatgatagt cattgatttc
     9421 tatttctaca ctactatatt tattttttat atagttataa atatcctttt gtctctctac
     9481 ttcaaattct ttatctcctg gatatatttc atgcgatatt ttgagactat catacttttc
     9541 ttttaaatac atttttattt tctcattaat gcaatctaaa catactatac ctattttatc
     9601 caatgctagt cctgccatca tgttgcaatt tttcatatag tcgttcatct tgatgtcaat
     9661 tttatcccct attgtataca ttacagcaaa tgccttttta cattctttag gtgttttatc
     9721 aatattaact tcacttatat atatcttaat atctaaaata tcatttatat ttcttatttc
     9781 tctttcaacg atttcactaa ttgtatcagg agcttttcta tttccatatc ctaaaaactt
     9841 tagtacaata ttcttatcta taaaaatatc cgctaaattt atatctgcta aaataaattt
     9901 atctttatta ctattataat tcattaatat caatcctatc taattcttta taactaattc
     9961 ttcctgcctt tatagctgct tgtaaatttt ctactggagt ggtatttaaa cagttacatc
    10021 caaaatcaca taaaaaatta tcatatcctc tcatcttctt taacaatttt agtgtctctc
    10081 ttctaatttt atctggttta gaacgtccaa gaagttttat tgggtctaaa ttaccaatca
    10141 acactatatc tctaggaatc attggagcaa tttcttcata gtctaaaatt tggtctaaac
    10201 ttactgcatc tatattgcaa tctaacatat tatctaataa ttctctggta tctccacata
    10261 tatgcatacc tttccaacaa tcaagttcat catagatttt atttaaattt tttactatat
    10321 attcatcaaa tctatcttta cttagagtta ctgatgttgg ctctgctatt gaaatatact
    10381 ttgctcctgc tttatttgca gccacagcat atctccttac caattcagtt gtaaattcca
    10441 acaattcttc tacaaatttt ttatcagtta ttatacatct taaaagttga gtagctccag
    10501 ctaattgacc tgcaagtgta aatggacctt gtatcgattc atatagtggc tttttaatac
    10561 tttttgatat cagtgataag ctttctatat tgagtggcat acgtccactc gtataagggt
    10621 ctggaacatc taattttctt aatttctctc tgtcagttat agtatgtgtc aatacacttg
    10681 gaaaatcata atctggtctt aatgtttcta aacccagagt ttcacaaaat gtaatcccat
    10741 cacagaatga gtatataaaa tctgattcaa aatatttgtc catagtcttt gctaattcta
    10801 attgcaaatc tacactatca tatacctcat atgccttttt atttattaag aacaatccat
    10861 ttgttcccat atcaggtaga aaaaaacctc tgtcttcact atttatatac tgaagtaaag
    10921 tcatatctga aaacatttaa acttcacctc ctataagttt atttattaat ttgactgctt
    10981 ttggagcatt tgaactatat ccatctgctc ctatttcttc tgcaaactcc atacttacag
    11041 gacctccacc aattattact tttggtcttt tatctacctt aatcctatta agttcatcta
    11101 taacagattt catattctcc atagtagttg tcatcattga agataagcct attatatcca
    11161 cattgttttt tatagcttct tctattatgt cttcactttt tctatttaca cctaagtcaa
    11221 taactttata tccagtggct tctaccatta cttttacaat attttttcct atctcatgag
    11281 tatctccttc tactacagac attagaatta ctccctttga ttttttcttt acttttccag
    11341 tttttttcaa aatattaatg cctttattta atgcatctgt gcatactata acttctgaaa
    11401 gatagtattc tttattttca aatttatcta aagctcctat catacctttg tttaaaccat
    11461 attcataaat atcttccaca tcaatatttt tatctatagc aatttttact aaattctcta
    11521 tattatctac acccatattt aatatacatt ctgctatatc atcaagtatg tccatataat
    11581 actcatcctt tctaattact tacaaaatta ccttttcaat atccaataat ttcttttatt
    11641 taacaaggac tagctctagg tatcaatcaa ttttatttaa tatcatctaa aagtacaaca
    11701 actattgata atttaaattt gcagtccatg ggaaatctac aggaaatgcc tttgtagcaa
    11761 atttatatat attttttaga tgtttaaatt cattgtactg ttcttcattt ttttcaaaaa
    11821 acagtttatt aagcttatcc aaactttcta ttacaagttc ttcttcttta tatttaataa
    11881 tatatttatt ttcaacttgt ttaaattcca tgtattttaa caattcagaa tctacatatt
    11941 gactaaaata acttcttaag ctatcacaca acttagtaaa atttattatt ttcatagttc
    12001 cttgttgata gtccaaagat tttatcccat tacatttcaa ctggtcttta agatttttca
    12061 catgtacttt ataatttaaa tattctaatc cgaatttatt tgcaacatat ttcaatatat
    12121 tttcaacatt tacagagttt aaccctattt cagcaacttc tcctacagta gagtcttctt
    12181 tttttattgt tcttattatc aaatatccta ttatattatt attttccttt ataaatacct
    12241 tattgtaagc aatcgttccc caggcaatag ttgctgcatg caatagtttt tgaaactcat
    12301 ctctcgttct tataaatcta gtactatttt gattatatag ttcaatcatt ttatctaaat
    12361 catcatcttt ttcaaaatta gtttcatcaa attcaactat ttcatatggt atttttacat
    12421 cttcaggttt tattgtatat ttatagaaac tctttaccaa actgcaattt cttctactgt
    12481 atagacttct tgttcctgat ataattacaa tatctacacc atcataaaac attttttctt
    12541 ctaccttatc caatatctta gatgaatagc ctctcttttc ataatctgga tgagtacata
    12601 ctccacctat agctgcaacc ttcaatctat ttccttgaat agatacatct tgaattagat
    12661 agtttacatc agaaaccact ttatcatctt ctgatattat aatcatattt tctatattat
    12721 ttttacttag taaaagagga aattcttcca tcatagttgg tttatgattt cttgatatcc
    12781 taaatacata gtttattaat tctattactt tatcaaattc ttctaatttt gcacttcttg
    12841 gtcttaattc cattcatatt cctccaaata ttatttaata tacttaaaac tttattatct
    12901 ataataaaac atttattact acagttctaa ttatatcttt tttctttaca atttttaatt
    12961 gtttttttca gcaaattttt ataaaatata aacctaattt ctagttaaac ttata
//
LOCUS       DAMJVM010000098        12905 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.102, whole genome shotgun sequence.
ACCESSION   DAMJVM010000098 DAMJVM010000000
VERSION     DAMJVM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12905
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.102"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            70..1863
                     /locus_tag="PC523_002891"
     CDS             70..1863
                     /locus_tag="PC523_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2476396.1"
                     /translation="MTIKDRLSGLRKLMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDGIWTDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYSDVYEFVKNIDKAEKVLVDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEIELENIRNSHIKDGVAFTKFMYWLKQNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKVKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(1945..3327)
                     /locus_tag="PC523_002892"
     CDS             complement(1945..3327)
                     /locus_tag="PC523_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2476397.1"
                     /translation="MSVLKTQEKLKQPKDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLSWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGASISMYGYRYVFLFSSITVLIGLFFFVFGNRKFYNSLN
                     NNHSNERESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVMQTGTDSNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISLLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSS"
     gene            3585..3932
                     /locus_tag="PC523_002893"
     CDS             3585..3932
                     /locus_tag="PC523_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2476398.1"
                     /translation="MIRKKSELQVEVKENLNGGIGNVKLENIIQNEELKGKGRLFKRV
                     SLPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            3950..4984
                     /locus_tag="PC523_002894"
     CDS             3950..4984
                     /locus_tag="PC523_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476399.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKEINSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLNESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARGKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(5033..6082)
                     /locus_tag="PC523_002895"
     CDS             complement(5033..6082)
                     /locus_tag="PC523_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2476400.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEIGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MIGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSVGKGGTSMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            6357..7892
                     /locus_tag="PC523_002896"
     CDS             6357..7892
                     /locus_tag="PC523_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476401.1"
                     /translation="MRFIKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKDPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNRVSAVGVGKATIYAKCGNKEVERTFRIEARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPIERAISLSSNGNIVGL
                     REGKDRVRITCGDKSKIITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNVDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMKVESISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNNSQNSDVI
                     NVR"
     gene            complement(8039..8389)
                     /locus_tag="PC523_002897"
                     /pseudo
     CDS             complement(8039..8389)
                     /locus_tag="PC523_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
     gene            8391..9452
                     /locus_tag="PC523_002898"
                     /pseudo
     CDS             8391..9452
                     /locus_tag="PC523_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            9853..11121
                     /gene="eam"
                     /locus_tag="PC523_002899"
     CDS             9853..11121
                     /gene="eam"
                     /locus_tag="PC523_002899"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HDF2476402.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEHIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTYIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPIYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(11214..11570)
                     /locus_tag="PC523_002900"
     CDS             complement(11214..11570)
                     /locus_tag="PC523_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476403.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(11721..12815)
                     /locus_tag="PC523_002901"
     CDS             complement(11721..12815)
                     /locus_tag="PC523_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2476404.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTIMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTTALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         5099 a         1602 c         2051 g         4153 t
ORIGIN      
        1 atttatgtaa taaactttac atacttatat atttagttat tcaaactatt atttttagga
       61 ggtcttgttg tgactataaa agatagatta agtggattaa gaaaacttat ggaagaaaaa
      121 aatatagacg cctatatgat tccatcttct gataatcacc aaagtgaata tgtaggagat
      181 tacttcaagt caagagaatt tatatcaggg tttaatggtt cagcggggac agttattgtg
      241 actaaagacg aagctggatt atggacagat ggaagatatt ttatacaggc agagtctcaa
      301 ttagaaggaa gtacaataaa attattcaaa atggggcaag aaggttgccc aactacagat
      361 gaatatttat acaaaaatat tcctgaagga ggaactttag gatttgatgg tagagttata
      421 tctgctagag aaggagctac attagctgaa aagttatcta aaaaaggtat aaagatagaa
      481 tatcaatatg atttaataga tggtatatgg acagatagac ctgctttatc agatagtaaa
      541 gcatttttat tggatgttaa atactgtgga gaaagtttta gctcaaaatt agctagacta
      601 agagaaaaaa tgagtgagaa aggtacttct actcatgtaa taactactct tgatgatata
      661 gcatggttat tcaatataag aggtggagat gttaaatata atccagttgt gctttcttat
      721 gcggtgataa ccttaaaaga agtttatcta tttgttgatg aaagtaagtt aaatgaagaa
      781 atattaaatg aactagctaa agaaaatgtt caaataaagc catatagtga tgtatatgaa
      841 tttgtaaaaa atatagacaa agctgaaaaa gttttagtag atggaactaa gcttagttat
      901 acaatttaca ataatatacc ttgtgaagtt gagaaagttg atgagtttaa tccagtaatg
      961 ttttttaaag ctcaaaaaaa tgagatagaa ttagaaaata taagaaattc acatataaaa
     1021 gatggtgtag ctttcactaa atttatgtac tggttaaagc aaaatgtagg aaagatggaa
     1081 atcactgaaa taagtgctac tcaaaaacta gaggacttaa gaagagagca agaaggtttc
     1141 tttgagccaa gtttcaatac aattgctgca tataaagaac atgctgcaat gatgcattat
     1201 agtgcaactc cagagtcaaa ttataaatta gaggcagaag gattattttt agtagattca
     1261 ggtgggcaat attatgatgg aactactgat ataactagga ctacagtttt aggacctata
     1321 agtgatgaat taaaactaca ctttacttct gtagcaagag gaatgataaa tctttctaaa
     1381 gttaaattct tgcatggttg tagaggatat aacttagata tattatctag aagttgcatg
     1441 tggaatatgg gaattgatta tcaatgtgga actggacatg gtataggatt tgtattaaat
     1501 gttcacgaag ctccaaatgg atttagatgg agagtagtac ctgaaagatt tgatagtgca
     1561 gttcttgaag aaggaatggt aactactaac gaaccaggta tatatataga aggttcccat
     1621 ggtataagaa cagagaatga aatcgtagtt agaaaagctg aaaaaaattt ctatggtcaa
     1681 tttatggaat ttgaggttgt tacattagct cctattgact tagatggtat agtaccagaa
     1741 ttaatgaata aggatgaaaa ggattattta aactggtatc ataagttagt ttatgataaa
     1801 atatcacctt tcttaactga tgaagaaaga gaatggttaa aagtttatac tagagctata
     1861 tagtttgtac tagatacaat aaaaaatcga ggtattataa tacctcgatt ttttattgta
     1921 tcttttatta attttgaaaa atttttaact tgataattct actaactttt tattcattat
     1981 aagtagtata attccaaata tcaataaaaa tgatggtatc actatgaata cttgtaacat
     2041 accaagttgg tctataatag cttgagtata accagataat ttatttgctg caaatttacc
     2101 taaaaaccaa acacccatta gtaatgaaat atacttttca ggcgaaagtt tatttaccat
     2161 accatatccg actggagaaa agcaaatttc tcctatactt tggaatgtta aaaacccaac
     2221 tacccaaaat acacttgctt tatgaattga attagagtcc gtacctgttt gcataactgc
     2281 tactatcata aataaaaatg ctatagctaa tagtataaaa cctaagctca tttttttggg
     2341 tacacttaaa tcgccttttt tagtttgaga taattttacc catactagag cagagatagg
     2401 tccaagaata acacaaagga gaccattata tgaatctatc catgaagtag gaatttgaaa
     2461 attgccaaca gtaagattta tatgctcttt tgtatagagt gcaatagaca tagaggcttg
     2521 gttatatgct atccaaaaaa atactgtgaa aaaagctaat accaatatta ctattattct
     2581 ttttttctca atcaaagtta atggtcttga agcgagcatt ttcttatatt ctagtgattt
     2641 tcttatagat tctctctcat tactatggtt attatttaaa gaattataaa actttctgtt
     2701 tccaaataca aagaagaata aacctattag aaccgtaatt gatgaaaata agaatacata
     2761 tctgtatcca tacatactta tagaggctcc acttttcata gcaaaccatt cattagcaac
     2821 taaaccacat attaaaggac caaataaagt acctagatta gtagccatat atgtaataga
     2881 gaaagctcca tcttttcttg ggtcatcatt ggtatataag tttccaacca tggtatgtaa
     2941 gtttccttta taaaaaccta ttcctaatcc agataaaaat atcatgatgt ttatatccat
     3001 cttactattt gctatgtatc ctagaaaata accaattgaa accattagca ttccaagtat
     3061 gatacagttt ttgggaccta gaaatctatc actgacccat ccaccgataa ctggagagaa
     3121 ataagtccat gataaaaaag atgcaagcat tgatgcggat tctgctggtg ttaaccctaa
     3181 accacctttg gagacttgtt cagctaaaaa taataataat accaatttca tgccatagta
     3241 tgcaagtcct acaaaggtaa atattgaaca gcagaaatat aaatctttag gttgtttaag
     3301 tttttcttgc gtttttaaaa ctgacatatg tcgagaacct ccagataaat agtttaacaa
     3361 aattttacca tactatatga gtatatgttt ttttcataga gacgtcaact tatgagataa
     3421 tttaattaag tcgaaatata tgtaaaaata gtatcatttt tgtgtattca ataatatttt
     3481 tttatgataa tatatgttat tattatatta aaatacaaac atatttatgg aaaggatatg
     3541 ccaatttagt agttagattg attgtttggc aggtgggatg agctatgata agaaagaaat
     3601 ctgaattaca agtagaagta aaagaaaatt taaatggtgg tataggaaat gttaagcttg
     3661 aaaacataat acaaaatgaa gagcttaaag gaaaaggaag actatttaaa agggtatctt
     3721 taccagtagg aagttcaatt ggtgtgcatg accacacaac agattttgaa gtatattata
     3781 tactaaaggg aaaaggcaaa gtatttgata atggggaatt tgtagaagta aatgagggtg
     3841 atgtagtgta tacagctgat ggcgaaaagc attctataga aaatatagga gaagaagatc
     3901 ttgagtttgt agcactagtt ttatatatgt aaaatatgga ggaggagtca tgacaggcaa
     3961 gatagaagaa attaaggaca agataaatag attttgcaac tccaatttaa atcaagaata
     4021 caaggatata agctttaaaa ttttgcaaaa ccttttagat aataataaag agataattca
     4081 ttcttctaga gctgatatat ggtcttcagc tatactaaat atagttttag aacaaaatct
     4141 tttatacaat aagaagcatc cactacatat aacaaaaaaa gagttttcaa aacaaatagg
     4201 tgtttcttta aatactataa ataataaatc atctttaata aaggatgttt gtagtattga
     4261 ctttttaaat caagatacta tagctatttc taatgtagaa ttttgggtaa aagagataaa
     4321 ttccaaatca aagtctgtag atttagattt agaaaagaag aaaatactat ataaaagata
     4381 tattaagcta tcacaagatg caaatagtga tattgaatca attagatata tggaggaagc
     4441 tgtaaatata ggtaaaagta tgattactaa agaagataaa gaacttggat tttggaaagg
     4501 tttatctacc agagcctata tgatagcact tgaatccctt gctaggaagt tagaaagtat
     4561 taataatcta aatgaatcca aaaaagtatt ggaatattta atagaaatta atcctagtga
     4621 tgagcaggga ataagatata aattattaaa tgtattaatt agattaaacg atagaacctc
     4681 aataaataaa ttatttgaac tttataaaga agaaaaatct gctacatgga tgtacagtaa
     4741 agctctttat tactttaaaa ataaaagtat gtttttggca aatgattcta ttaaggttgc
     4801 tagaggtaaa aataagtatg ttggattata tttgttagat tggcgaaatg cttttggtag
     4861 agaatttgtt acagaagaag aaaaggcaga agctgtatat tactatgatg aaaacatagt
     4921 gatttggaat gaagttaaag gttctatgga ttggctatta aaaaaaatgt tggaattttc
     4981 ataaaaaaaa ctactcctta cttttatata agtaaaagga gtagttttta atttacacat
     5041 ctagttttaa atcttcaggt tttattagac ctttttttct cataaattct gaaatcaaca
     5101 aagttacaat agcaggaagt ataaaattaa gtaaaagtat accaacccac atagatgtac
     5161 ctccctttcc aacactttgc atagcagtca ctgttccaaa ttgtccaacc aatccacaag
     5221 tacccatacc agagccgata ggaatattct ccattttgaa tattgttgta gataatggtc
     5281 caagtaatat tgaagctaca gtaggtggta tccaaatttt ccaatttttt attatattag
     5341 gtacttgtaa catagatgta ccaagacctt gtgccacaag gccaccaact ccattttctc
     5401 taaagctcat aacagcaaag cctatcattt gacaacaaca acctacagtt gctgccccac
     5461 cagcaagacc acccaaactt aacatcatac aaatagctgc actacttata ggtaatgtaa
     5521 gagctattcc gacaagtgca gagactaaag ctcccattat tataggttgc atagttgtag
     5581 cttgcattat tatatttcca aatgaagtca taaaagcaga aacagctgga ccaataagct
     5641 cagctacaag tactccaatt agtattgtta cagcaggtgt aaccagtata tctatttttg
     5701 tttcttttga aactaatttt ccaatctcta cagcaaaaag tgtagcaaca aatgctccaa
     5761 caggtcctcc taattcatta ccacaagctc caacaagtgc acaagaaaat agtacaagtg
     5821 ggggtgcttg aagtccatat gcaatagcca ctgctatagc tggaccggta acctgtttag
     5881 caagagggct tataacctca gtaaaaagag aaatattgaa attatctcct aatgtattaa
     5941 aaattgttcc aattaaaagg gaagaaaaaa gaccgagtgc catataactt agtgcatcaa
     6001 taagatacct ttgaacagaa atttcaatgt tttttctttt agcaaatgat ttgaaatttc
     6061 tttttttata attgatttcc aatttaaaat cctcctcata taatataagt ataatttctt
     6121 tttaattaat tatataactt ttcagctgac aagacaaatg ttatttcact aaaattagac
     6181 tattgcaata gtaagaaatt aacattaatt ttagttttaa attttaaatt tatggtttat
     6241 aattagtata gggatattct gaaatataat aattttccag tttatcccaa tatttaactt
     6301 aggtagattg cttacaaaac aaatgattat atattaagct aaaaggaagt ggatagatga
     6361 gatttataaa aaataagaaa tacagaaaaa ttgctataat tatatctatt cctatattgc
     6421 ttgttgcgca gtactacctt tttaaatttg gaatacttag accaatggta aagggagtag
     6481 aaattgaaat tgtagatggg gaatatgtta aagatataga taagtatgta gtaaaattaa
     6541 atgaaacagt tactttatcc actggtgaat atattaaggt tccttcgtat gctaaagatc
     6601 caaatatttg gtttaatgta ttggataatt caggaacttt aaaaatagat ggaaataaaa
     6661 tgacagcatt aaaggttggg tattcttctg tgggtatcat gaaaaattca agagtattaa
     6721 aaaaggctac tataaaagta gtagacccag aggtagagtc actagatatg gagattaatg
     6781 gagatttaaa acatgttggt gatagtgcta ctatagaaag cacaatagag gttgattatg
     6841 acaagtttaa aaaatcatat aagccagttt ataagagtag taatgaaaat attttaaaaa
     6901 tcaaaggaaa tagagtaagt gcagttggag ttggaaaggc tacaatatat gctaagtgtg
     6961 gaaataaaga agttgagaga acatttagga ttgaagctag agttgctgaa ataaatatgg
     7021 atgatataaa aatagaagtt gaccaaaatg aaaaattgaa accagatata ataacttctc
     7081 cacctggttt agaaccacca aaaatatatt atgagttagt agattcaaaa ttacctatag
     7141 aaagagcaat aagtttaagt agtaatggaa atattgtagg gcttagagaa ggcaaagata
     7201 gggtaagaat aacttgtgga gataaatcta aaataataac agtaacagta gtcaaagaaa
     7261 ctataacgaa tgactatata caaaatttgg ttggctcaca taaaatagta aataataaag
     7321 ttattattaa acttgaatgg gattatatga aagatgtctt tgattatgat atatatctta
     7381 aagataaatt gtcaggagaa tcatttaaga aaattaaatc tataactata aatgaaagtg
     7441 atttaggtaa gtctaacaaa gtatatgcaa ctatagaagt agatatgaag gggaatcaaa
     7501 acgtagattt tgatatctat gtcataggga aaaaggattc agatacaact aagccaagta
     7561 atgtaataaa tattactaat gggaatggaa attcaaataa tgataataac attaaagaca
     7621 tgaaagtaga aagtatatca gctaatgttg atagagataa taatgttatt agagtcaact
     7681 ggagtagtat atctcataag gattgtactt acagcatata tgtaaaaaat aatactaatg
     7741 gagatggaga atttactcta tatcaaagtg atatacaggg gaatgagcat actataagta
     7801 ttcctgaagg agacttaaat ctagatattt atgttgttgc aaattataat gggaataact
     7861 ctcaaaatag tgatgttata aatgtaagat agatttgtat acaaatgagt attaagttta
     7921 ttgaaaaatt aaattaagaa aaaggatagt gtgatagcaa tcatactatc cttttttgtt
     7981 aaaatataga ggtttcaaat ttttagttaa aaaaattttt ttattaattg atattgtatt
     8041 aaattgaact tagtgctgct tctattgcat gtattattgt tgtatcaaat agtggaatag
     8101 aagtatcatt ttgtttaatt aaaagacctg tttctgtaca acctaatata acaccctctg
     8161 caccttgctt gccaagctta tctataatag ataagaaagc tttctttgaa gattcagata
     8221 caattccaac acaaagttca ttaaatattg tattatttac tattatacgg tcttcttcat
     8281 ctggaattaa aacatcaatt ccattattaa taattatgtt tttataaaaa tcttgttcca
     8341 ttgtatattt agtacctaat aaagctactt tttttatttc tttttccttt gatgtaaatg
     8401 taagtaatac attattatat caaacagaat caaaagacat aattgaacta ggaacagttc
     8461 atcctccata tgagcaaaat acatatatta ttgattttat taaaagtata ctaaatcaac
     8521 cagaaataga taaattttta caatatatgt ctccatctgg tacgtacatg caaaaaaaat
     8581 caattgcaaa atggatagag agaaataata tatacactag tgaagaaaat atcttattat
     8641 ctacaggagg gcaaaatgct atatgtgcaa ccttactagg attatttaaa gcgggtgata
     8701 ggattgctac agattctttg agttttctgg aattaaatca atagctaaaa taataggtat
     8761 tcaattggtt ccaattcctc aagataataa tgaaatgtcg attgaacatc ttgaaagtta
     8821 ttgtaaaaat gaaaatataa aaggcatata tcttatacca gattatcata atccaacgac
     8881 acattctatg tctgatttat cttgtaaaaa gatagcaaaa caataaatat aataatcatt
     8941 gaagatgcca taaacagtat ttttagagat agtacacaaa ctcctatgtt ttctttggcc
     9001 agtgataata ctatatatat tttttgtaca tctaagtttt tatgtgctgg tcttagaata
     9061 gcatttgtag ttgccccaaa acgatatata gaaaacttag aagatgcact atataatatg
     9121 aatctaatgg tttctccatt tacggcagaa atagtacacc gtttactata ttctcctatt
     9181 ttagataaga ttataaaaga gaaaaaagat acaattatag aaggaaataa agttgctgat
     9241 aaagttttgt cagattataa tttgatgaat tggagggaaa gtcatttgaa atatctgcaa
     9301 aaaatctagg tgttcaagtg tattgtgcag aacgattttc agtaggcaac tctacagtac
     9361 caaaagcaat ccgtatttgc ataacagctc caaaagatgt agaagaatta gaaaaagggc
     9421 tcaatataat aaagtcactt cttaatagct aaatttattt tactatgatt taattaaata
     9481 aataaaaagg cggagaaatt tattataata aacctccgct tttattattt aatgaaatac
     9541 tttactttct attaaaaatt taattttaat aatcttgaaa aaataatcgt tttatgataa
     9601 aataattctt aaaaatagaa aaaattaatc atttgttaaa aaaaatgcat aatatgttgt
     9661 aatttattga cgaaagtaga aaaatattca taaatgtgca aaataataga aaaaatgcaa
     9721 aaataagttc aatttcattt gttataaaat ctatatggca aaatcaaatt tgcctatcag
     9781 aatgatataa acacatcaga aaggaggagg ctatcaaaca atataagtag ttaactttga
     9841 tagcaaaaaa ttatgaatga acaaactaga atatccttag agagagctgc tgaactaaaa
     9901 tcaaaaattg atgattatat tcaggctaga aaaacgatta acagaggtct tgaaaaagaa
     9961 gaagagataa ataaacgaaa acagaaaata ttgagtatat taaatggaac tgaagaggat
    10021 tggaacaact acaaatggca attatcaaat agaataacag atgtagatac tttatcaaag
    10081 attataactc taactaaaaa agaaaaagaa catataaaag aggttggtac tcaatttaga
    10141 tgggcaatat ctccatatta tttaagcctt atagacccag aagatatatg tgacccaata
    10201 aaattattgt ctataccaac atatatagag ttagaagatg agcaagaaga tttggaccca
    10261 atgggagaag agtatacaaa tccagcagga tgtataacta gaagataccc agatagatta
    10321 ataataaatg taacaaatga gtgtgctatg tattgtagac actgtcaaag aagaagaaat
    10381 attggacaac aagattctca taagtcaaaa gctattatcc aagaatctat agattatatc
    10441 agagaaaatg aagaaataag agatgtacta gtaactggag gagatgctct tacattaaaa
    10501 gatgattatt tagagtggat tcttagccaa cttaaagaga taccgcatgt tgattatgtt
    10561 agattaggta ctagaactct tgttacaatg ccacaaagaa ttacagatga attttgcaat
    10621 atgctaaaaa aatatcatcc tatatatata aacactcatt ttaatcatcc aatggaaata
    10681 actaaggagt ctaaagaagc ttgtgaaaag ttagcaaatg caggagttcc attaggaaat
    10741 caggcagtat tattaaatgg aataaataat gataaatttg taatgagatg tttaaatcaa
    10801 gaattactaa aaataagagt aaaaccttat tatatattcc aaagtaaaca tgtaaaggga
    10861 acaaaacatt tcaatacatc agtagatgat ggtcttgaaa tcatggagta tttaagagga
    10921 tatacatcag gaatggctat accaacatat atagtaaatg ctccaaaagg aggaggaaaa
    10981 actcctttgc ttccacaata ccttgtatca aaaggaacag attatgttat gcttagaaca
    11041 tgggaaggaa aagttataaa aatggaagat gaacctgctg tagatataaa gaaacttata
    11101 aaagaacaag cacaggatta aaacagtatt taaattctat aaaaagaaac aactttaaaa
    11161 taaaaagaaa atggatgcta aatttatatc aatttgcatc cattttttat tagttattta
    11221 ttattttgtt gttgattaac taattcaaga tatccacttg ggtggaagta atatatctca
    11281 gaagtatttt catttacaaa gtaaccaata tcgccatctg catctggtaa aaatacataa
    11341 ccattaagat ttttagcttt taatgcttca aaatcactag catttccttg atatatgtaa
    11401 tcaacttcat tgttgtattt tactaaaagt tctttagctt gctcttgagt caatttagta
    11461 ttttggctta caggaacatt cttttgagaa tctgcgtaag aagattcatt tactggtgca
    11521 gttaataaca aacaagataa aactaataaa gatataagtg cttttttcat actaaaaccc
    11581 tctttcatgt ttaattatat aagattcatt acttaataat attgtatgtt tcctataatt
    11641 aaatattacc attaaagaga ataatattac agttacacag ctgtaacttt tgattacaaa
    11701 atcgtaatta aattgtattt ttattccatt tgtattttat catgtctacg atttacaaac
    11761 tcacaaaaat atatttttat aacgcccata acaggagcag atagaatcat gcctggtata
    11821 ccaaagaatc ctccgccaac agtaacggct aagattgtaa agaatgggct aagacctagt
    11881 tgtccaccta cgatttttgg ctctataaat gtaacttcta cttgttgaat tattattaag
    11941 taaattaaag aagttaaagc agttgttgga ttggagaata aattaattat tacaactggt
    12001 gccataccta ttactggacc aaaatatgga accatattca tgacacccat aattgtacca
    12061 aataacagtg catattcaga ttttaaaaag taaagaccaa ttccagaaag tactccaact
    12121 ataaaagaat ctaaaatctt acctgtaaaa tatttaccaa tatttaaatt taagctttga
    12181 catagttcta ttataaagtc tccatatttt ttacctaaag aaatataaac tactttcttt
    12241 gtaaatagta agaatttttc tttttctaat aatatataga aacaaattat aaaacctaaa
    12301 agaaattgta ctataaactt tccaaccgag aatgtagtgc taaatattcc gccaagtgaa
    12361 ccaataagta agttactaaa tttaggcatt acactcataa ttttgtcacc atattctttt
    12421 aatgtattag ggtcaactga ttgtaaactc ttgcctaagt caaataaaaa ctgttctgtt
    12481 ttatctatgt atgtaggaat ttgagcaact aaatcagcta aactatttac tatggatgga
    12541 actgtaaata atataaagga aattagtata actatgagta caccataagt aagaagtaat
    12601 gaatatattc tcttaaaatt taatttactt tctaagaatt ttactagtgg gttgaatata
    12661 tatgctaata caaatgctat tataaatggt gttagtagtg acattaataa acttatcact
    12721 ccaaaaaaat atttgtaatt atcaataact ttaatcaaga tatatgaaac aactacagtg
    12781 agtacaatat ttagataaat ttttttatct tgcaaaataa cacaaccttt cttatattta
    12841 ttaaatttag atatacttaa atctaagtta aagttaatat ttatagctta ttatacaata
    12901 aaaag
//
LOCUS       DAMJVM010000099        12884 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.103, whole genome shotgun sequence.
ACCESSION   DAMJVM010000099 DAMJVM010000000
VERSION     DAMJVM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12884
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.103"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            219..929
                     /gene="rgaR"
                     /locus_tag="PC523_002902"
     CDS             219..929
                     /gene="rgaR"
                     /locus_tag="PC523_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HDF2476405.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
     gene            1957..2988
                     /locus_tag="PC523_002903"
     CDS             1957..2988
                     /locus_tag="PC523_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF2476406.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGLT
                     LDVDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLAIRNHWNSCDFDECSRIVNFKLDLYDKENDLLIKVADVVRDCL
                     NIEDNNEKLFKCSLFNKEIGLVYDNCFNLLKKLEYRFDVKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            3121..4419
                     /locus_tag="PC523_002904"
     CDS             3121..4419
                     /locus_tag="PC523_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDF2476407.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVREGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVNFDDVNIKKSDINSQIYDCTVMKENYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLIKTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKD
                     RKLTLLIGMLEDKDIDSVLEVLLPHFNKVITTTPINPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(4539..4784)
                     /locus_tag="PC523_002905"
     CDS             complement(4539..4784)
                     /locus_tag="PC523_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HDF2476408.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            4932..5798
                     /locus_tag="PC523_002906"
     CDS             4932..5798
                     /locus_tag="PC523_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDF2476409.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(5802..6338)
                     /locus_tag="PC523_002907"
     CDS             complement(5802..6338)
                     /locus_tag="PC523_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HDF2476410.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(6529..6732)
                     /locus_tag="PC523_002908"
     CDS             complement(6529..6732)
                     /locus_tag="PC523_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HDF2476411.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            7023..7787
                     /locus_tag="PC523_002909"
     CDS             7023..7787
                     /locus_tag="PC523_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2476412.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKKTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESRPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            8177..9490
                     /gene="prdC"
                     /locus_tag="PC523_002910"
     CDS             8177..9490
                     /gene="prdC"
                     /locus_tag="PC523_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HDF2476413.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQDDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      9893..9978
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            10509..12623
                     /locus_tag="PC523_002911"
     CDS             10509..12623
                     /locus_tag="PC523_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HDF2476414.1"
                     /translation="MNTIYVFSIRRILKTKIKKSSIISVVIAFSMVFTAFTPIVSYAD
                     EVIGNDTIINGEKPLEGQTPEGEKPLEGQIPEGEKPLEGQIPEGEKPLEGQTPEGEKP
                     LEGQTPEGEKPLEGQTPKGEKPLEGQTPEGEKPLEGQTPEGEKSLEEEIVIEEMSLKQ
                     DIDKILDMTLSQINKIVYNFWEDEENVKSDEQSEINQVFTSKDSFISLWYDKKAKVKN
                     TCLLKEIDGSDRPYHDLRFDDITKTLTFDYVIKGLVGASSKDNKYVIEGEDGEQTAFC
                     YNNHLRPPSSNGKSPYLPAEEFSGQENQNEDAVKSILYAGSEFDGFGYKQQFNLGGEE
                     NDIMTYSATQSAIWIILGQVDEQEKLKQYQGSINFCDFLIENAKNEEEKAEYQKKKEV
                     AINTKAYLEALLKAGREELKPNDTGKPSLSNGLTKINFEKNEDGTYETEAVALVGYSG
                     VVKLRLPDGVTAYDGDGNIIGTGEVEISTQQKFKLKSVGKPDAKANISAISYDYIFPK
                     AIQYYKAVIDLGQKDHSSSLPSSKQNLLSYTVEKKNGQEVNFNISLPTDDVVNPPVPP
                     TDDTVINPPVPPTDNTNGHKPKPSPPIDDTVINPPVPPTDDTVINPPVPPTDDVVLNP
                     PVPPTDDAVLNPPVPPTDDAVLNPPAPPVSDTVEKMPELSKYDTIVKSPKTGDETQLM
                     SYVFISIIAICGLAYQFKIKRN"
BASE COUNT         5211 a         1367 c         2158 g         4148 t
ORIGIN      
        1 acatattata tttattttac tgtatttttt tacataatat ctctagaaaa gtataggaat
       61 ttatcgtaat ttaagtttaa atattaaaga gaatttctat aggtagttta atcgacaatt
      121 taaatttata aagaaattta aatttgaaag ctagtaagta cagtttggaa attgaaataa
      181 agtaaactaa atgatatttt agatttggag gtgatattat gttcagggta gttatatgtg
      241 atgacgaaaa aatacaaaga agcatactaa aagagtttac agaaaaactt ttgtctgaaa
      301 gatgtctaaa ttatcaaata ctggaatttt cctgtgggga agatttaata tccaaatacc
      361 cagaaaaaat tgatattata tttttagata tccaaatgaa agatatcaat ggaattgagg
      421 tggctaaaaa aataagaaag tttgatgaaa aagtagaaat tatatttaca acagcttttt
      481 cagagtatgc tccccagggt tatgaggttc gtgcttatag atatttagta aagcctatag
      541 agtatagtaa ctttgcaatg ggtgtaaatc tttgtattga taaccttcaa agaaagaagg
      601 agcgttatat agttttaaat agtaaaaaag gatttaatag aatactgata gattctattt
      661 tatatgttga aactgtaaag agagatttag ttgtacatac aataggtaga gattataaag
      721 ctaatatgag catgaaacaa gcagagaaat tactgtgtga aaatggcttt tttagatgtc
      781 atacaagttt tattgttaat ttaagaagaa tagaagatct tagggataat atgattacta
      841 ttaatgaaca atttatacct ataagtaagt atagacttaa agatttaaaa gtagcattaa
      901 caagcttgtt atgtgatata atgtattaat tttaaaaagg taagtgaaaa tatcgtaaaa
      961 aatatttaat gtcatcacag ggctattgat taaaactgtc ttaatatata aaagattatt
     1021 tatattaaga cagttttatt atgtaaaata gtagtttata gacttttaat attgtttaat
     1081 atgtatatgt agaaattaca ttatagtagc ttgattatta atatgctatg attgattttt
     1141 atttatagta tatgcctttt tcgaatatgc ttactttttt aaatttatga ctatttataa
     1201 agctatatag atatttgttt cattttacta tgtatacttt aattaattat attaatattt
     1261 aaggaagtac aatataaata ttaaagtgat atcttatagt ttattaaact tatagtagtg
     1321 gactaataaa tagtttaatt ctatgaaaag atttcctttt atgcaattta tttattgaaa
     1381 tatgatataa tatatgaagg tataagtatt agttgttaag ataatattta tattagtttt
     1441 tatgtatact tagtgcaagt ttttcatata ttgattaaaa ttgatggtga tttattattt
     1501 ttagtatttt ttcgcctttt gattcatact ttgtagaatg gtgtattttg taaaactgat
     1561 tataagacaa gtttattcaa attagcattt ttatagcata taaatgattg ttccccctaa
     1621 ataagactta gatatatagt attaaaatca aaaagtataa aataaaattg atgcttaatc
     1681 atagataaat attaataaaa tatacaaggg gattgtctaa taaaaaagat aatctccttt
     1741 tttacaaata attctaaatt gtgatataaa tctaaaaatt ttatcttaac atttaaccat
     1801 ttaatgataa taattgatat aataaatata aattaaatat atagtgtact tattttatgt
     1861 ttttagcaag aaatatagca tttaaaagat aaattgtgca acttaaatca aagtttaaga
     1921 tgaactatta acatagatat attaaaaact tttgtaatgg agaatatatg tgatatgaat
     1981 aataaagtta aggttgcagt attagataca ggaatagaca aagaacatga ttacttaaaa
     2041 gataatttag ttggaggaat tgcttttgaa tgtatgcatg attatatctt tatatcagat
     2101 aaatttgatg atgaagatgg tcatggaact gcttgtgcat caataataaa gaaagagtat
     2161 gaagatgtag agttatttgt aataaaaatt ttgagaaaag atggtattac aaatataaaa
     2221 gttcttgaag aagcattaag atacctttta gacactaaca taagattaat taatttaagt
     2281 ttatcagtca taggagtaga aagtgttaag ggtttgtttg aagtttgcta tgaattgttt
     2341 agaaaaggaa agataatagt atgttcactc gctaatgatt ttgacttaag ttatccagct
     2401 atgtttaaca atgttatagg tgtaagggga cttactttag atgtagatga ttcattttgg
     2461 tacaataaaa aatatgatgt tcaatgtgtt atggatagca attcatatat aagttgtgac
     2521 ataaataatt cctatagatt gccaccaaaa tgtaatagtt atttgtcagc aaagcttact
     2581 ggtaaaatag ctaagatatt atcagaaaaa ccaggcataa caatatctga tttaaatgat
     2641 aagcttgaat cattagcaat tagaaatcat tggaatagtt gtgattttga tgagtgtagt
     2701 agaattgtaa actttaaatt ggatttgtat gacaaagaaa atgatcttct tataaaagtt
     2761 gctgacgttg ttagagattg tttaaatatt gaagataata atgaaaaact atttaagtgt
     2821 agccttttta ataaggagat aggtttggtt tatgataatt gttttaattt attgaaaaag
     2881 ctagagtata gatttgatgt aaagtttaat tatatggata tctcaaaata tgacttagtt
     2941 tcaatatata cactgactga attagtggaa agatataaaa acttataaat aaagatttgt
     3001 agctaaattt aatgtagaag gtattaaatt atatatatcc tttgtcagta gattaaaagc
     3061 aaaaaatata ctataattag atagcataag agaaaatgcc aatacagggg gaattagata
     3121 atgaaatatg aagaagcatt agagtatata tcacaaacta ataaattcgg tataagatta
     3181 ggtctagaaa atataggaaa gttattagaa ttattaggaa atccacaaga aactttgaat
     3241 ataatacatg tagctggaac taatggaaaa ggttctgtgt gttcttttgt ttctaatata
     3301 ctaagagagt gtggatataa agtcggttta tatacatctc catatttaga aacttttact
     3361 gagcgtataa gggtaaatgg acagaatata ccacaggatg atgttgctag aataattgaa
     3421 ttaataaaag aaaaaataga gattatggta agagaaggct atgcatatcc aacagagttt
     3481 gaagtggtta ctgcaatggc attttattac tattcagagc aaaaggtaga ctttgtggca
     3541 ttagaagttg gtttaggtgg cagatatgat gccacaaata taataacaaa atcacttgta
     3601 agtgtaatcg tatctataag tttggaccat acagggatac ttggagatac aatagaaaaa
     3661 atagcgtatg aaaaggctgg aataataaag gaaaatggag tagtactagt atatgaccag
     3721 actgatgaag caaaggatgt tataaagtca gtttgtaagg agaaaaaagc taaatatata
     3781 gaagttaatt ttgatgatgt aaacataaaa aagtcagata taaattctca aatatacgat
     3841 tgtactgtaa tgaaagaaaa ttatagagac cttgaaataa aattaatagg ggaacatcaa
     3901 ataaacaatt ctatattggc aattagtgta ataaaatatc taaaagacat aaataaatta
     3961 gcaaatataa atgaagaatc tataagaaaa ggacttataa agactaaatg gccaggaaga
     4021 atagaaaaga taaaagaaaa tccaattttt ataatagatg gtgcacataa tgaagatgga
     4081 gcaaaatctt tggcaaaagc acttgataag aattttaaag atagaaaatt aaccttatta
     4141 ataggtatgt tagaagataa agatattgac agtgttttag aggtattgtt accacatttt
     4201 aataaggtaa taacaacaac accaatcaat ccaagggcca taaattcaga tatattaagg
     4261 gaaaaagtat taaaatatgt agatgatgta acatcaaagc atgagataga ggatgcagta
     4321 aattatacat tagaaacgtc aagtgaagat gatattataa taagtgcagg ttcattatat
     4381 atgattggta cagtgagaac tttagtaaag aaattataat tttataaaat aaatatacaa
     4441 atataaataa aagtataaaa gagagttgtc taaaaaatgg acaactcttt ttatataaag
     4501 taagttttta ataattttat tgatatttat agaagcaatt atatagattg acgtatagct
     4561 ttagataatt ggtctgggca agaagtacct tttccaccac aatcaatacc gtttaattta
     4621 tcagcaactt caagagcatt ttgaccaatt attaagcttc taagtcctga taaattacca
     4681 ttacatcccc ctataaattc agcatcaaca atcacattat tttcatcaac ttcaaaaatc
     4741 atttctctac aacagacacc acttggatta aatgttactt tcattaaaaa gacctccatt
     4801 aaattttatt tcttttttta acattaataa taataccaaa tatatgagta ataatgcaac
     4861 tataaagcat aatacaagct aatgaaataa tatattattg tagtatatgt attgtagaga
     4921 ggaggggtta tatgaaaatc tatatttcta actatttatc aaagagcatt agcatagttg
     4981 attattctac acttgaacta gaaaaagaaa tagtattaga agataatata tatccacacc
     5041 atttttgcat agaaaaagaa aaaaatttaa tatacatacc tagttcaagt aatgggatac
     5101 tctatgtctt agatttaagc aatgataaaa taattgatac tgtttctata ggtggaagtc
     5161 ttagtcaagt tgtacttagc gataatgaat tatttgtagc aaatgaagat tctaatagta
     5221 tatatgtact agataaaaac acattaaatc caataggaat tattggagtt gataatatgc
     5281 cacatggatt tgactttgat agagaaagta aaaagttata tgtaccgtgt ataaactcga
     5341 tagtatgtat agatacaatt aataaaaata tacaaaagaa aataaatatg gactttagag
     5401 cttggcatat agaacttgat aaacaaaaga aagaaatgta tatatcaaca cttgatggaa
     5461 agcttataat aatagatgaa gtaagcatgg atataaaaaa ggtattatat gaatttttat
     5521 taccagtaga aataagattt aattatagtg gcaaaaaagt ttatgttgct gatttgggat
     5581 ataataatgt aagaatttta gattatacaa cgagtagata cattggaaat attgaaataa
     5641 atgggattcc tcagggctta gaaatatcta aagatgaaaa gttactattt gtatcagata
     5701 cacaagaaaa ttcagtaaaa gtttatgaaa ctgcaaataa caaattaata aaagaaatca
     5761 aggtaggaaa agagcctaca actataattt gtttgtaggc tttaatgtat gtctcttata
     5821 agtaagtcga taacttcggc atacatataa gatgcattat ttttccagtt attgcaaata
     5881 agttttgctt gtttctctga tgaaacattt agatttaaat ctattaagtt agactgattt
     5941 tctatgactc taaggttaac aataaattca ttatcacttt gttttacaaa actagacttt
     6001 acttgagtat ctgctagtaa attttctttg ttagaatcta tatacttgtc tattttttca
     6061 gtaacactag taggtattcg attaaagaag tattccagac tttctacgcc tctttgagta
     6121 agagcataat attctctgtc agagtcttta tatgtagtca aaaacttaga ttccataagc
     6181 tgagataaaa gctgttgtaa tgaaaaataa ttcatcattt cagtctctaa tactacttga
     6241 gttatttgag aattagttaa atccatttta attttttcta atatatataa aattaaaagt
     6301 ttgtgatagg ctaattcttc agacgagttt tcaaacataa atatcatcct ccgtcaaact
     6361 atttctatat ataatatatt aaactaattt gtacaaaatt aaaagaacaa tttgtattgt
     6421 attattaatc tttaataatt catataaaaa caattttata aataaatata gagaaacagg
     6481 gctactaatt agcagccctg ttgataccca ggtgtcaaaa tttactattt attttccagc
     6541 catttgtctt tcagccattt caactaattt cttagtcata tatccaccaa catatccatt
     6601 ttctctagct gttaagttac ctttgtctat gttttcatag ttagataaac ctatttcatt
     6661 agctatttct aatttcattt ggtttaaagc agcttttgct tctggaacta ctgttctgtt
     6721 tgaatttgac atagtaaatt tcctcctcaa ataattagtt tatgatgtct aacaacactt
     6781 ttatttatgt gatgttaata ctaatttctc caaaataaaa ataatatata cagaaaaata
     6841 aaggtaaaaa tgtaaaaaat ataaaaaaat taaacaaacg agtattttag agagtttaat
     6901 taggtcaata taggttttat ttttttagtt attgtcttat aaattaaata ttacagtact
     6961 tttttgagct gaaaaaaaat aaattttaaa tatagaagaa attatttaaa agggggaata
     7021 gtatgaaaag agaagttaaa aggactaaag ctttgatata tagtgtttta tttttttcat
     7081 tattattagt gttaatattt gtttttgttt atggaataaa atacatatgt tctaaatctg
     7141 acaattcaaa aatgcctata tataaagttg atacaaatga gaaaaaaata gctttgagtt
     7201 ttgatgtagc atggggaact gataatatgg atgatatttt aaaaatttta gataaacata
     7261 atgtaaaagc tacctttttt ttagttggta gttgggtaga tgataatgaa gaaattgtaa
     7321 aagctataga tgaaaaggga catgaaattg gaaatcattc aaatactcat gccaatttga
     7381 aggaaatatc aaaagaagac ataacaaaag agatagaaac tacatcagaa aaaatatata
     7441 atataacagg aaaaaagact aatttattta gacctccttt tggggatgta aacaacaaag
     7501 ctatggacat ttgtagtgat ttaggttata aagtaattaa gtgggatgta gattcgatag
     7561 attggaagga gcttggacca aatcacgtaa ttgaaaaagt aataaaagaa tctcgaccgg
     7621 gttcaatagt tttatttcat gcgaatataa atgatgtaga taactatttg gatactataa
     7681 taagtcgact aaaaaaagat ggatacagtc tagtcaaaat atccgatttg ttgtataaag
     7741 ataattatgt agttgattcc aatggagtgc aaaaaactaa gaattaataa gtaataaatg
     7801 tatattttaa gcaaagttgt ggtaaattat atagtatatg ttagaaaagt gtcgataaaa
     7861 agacggtgtc tattttttga cactttttat acatgtatgc aatatcaata attatagatt
     7921 aaattgattg aaaaaagtgt ctaataattg actttttttt gacgggctta aaaatttaga
     7981 agaattaata aatgctgtaa gatagtattt ccaaagggta taagtttatt tgaaagttgg
     8041 catagaaatt gctttgttaa taagcgatat gttctataga aataaacgat atgttaagac
     8101 cttataatag aaaaatagca ttaacattat ttgaacagct tataaaaaaa ttaatatact
     8161 taagggggcg tactagttga acaaactata ttctatttta ttaaaacaac acgttggtgg
     8221 tccagataag ccagtagtaa gtgttggaga tgtagtaaaa aaaggaactc taatcgcaga
     8281 accaactgga ttaggggcaa atatttatgc cagtgttagt ggaaaaataa gtgaaataaa
     8341 tgaccaagcg atagtaattg aagctgatga agtacaagat gatacttttg aaccattaaa
     8401 aggagaaggt atacttgact taattaaaga agctggagta gtaggaatgg gtggagcagg
     8461 attccctact catgtaaaat taaacataga tttaaatggt ggaacaatat tagcaaatgc
     8521 agctgaatgt gaacctttat tagctcataa tataaaagaa atagaagaaa gaccagaaat
     8581 agtttatcaa ggtataaaat atgctatgga agttactaat gctggaaaag gtatgctagc
     8641 aataaaaagc aaacatccaa aagcaataga agctttcaaa aaagtaataa agccaggaga
     8701 caacatagaa gttgcagaac tagttgatat gtacccaatg ggagaagaga gagcaatagt
     8761 aagagacgtt ttaggaaaat tacttgagcc aactcaatta ccttcagagg ctaatgcagt
     8821 agtaataaac gtagaaacat taactagaat agtagaggct gttgagcaaa aaagaccagt
     8881 tatatctaaa aatataactg tagttggtca attaaatagt ggaaaagagt ctatagtatt
     8941 tgaagatgtg ccaattggaa caactgttgg agaattaatt gaaagagctg gtggaataaa
     9001 aggtgaatat ggtgaaataa tcttaggagg acctttcact ggtaaagcta ctactttaga
     9061 tgctccaata actaaaacta gtggtggtat aatagttact atgccatttg ttaatgaaaa
     9121 gagaaaaatg ggtcttttag tctgtgcatg tggacctaat gaagagagaa tgagagatat
     9181 agcaactaaa atgggagtta ctgatatagt atcagttcaa aaatgtaaac aagctcaaga
     9241 aattaaaggt gcattaaaat gcgaaaatcc aggacactgt ccaggacaag ctcaaaaatg
     9301 tatagaattt aagaaagctg gagcagaagt tatattaata ggaaactgta ctgactgtag
     9361 taacacagta atgggttctg caccaaaatt aaaattaggt gtatatcata ttactgacca
     9421 tgtaatgaga acagttaatc acccattaat aagaagaata aaaggtgatg taagattaaa
     9481 aataaaataa tagataaata gaagctaaac atccttagaa gtaaaactta aaagttactt
     9541 ctaaggatgt tttttaatgt actttgaggg taagatatat tttaaaataa atggaaaaaa
     9601 tagacaaaat atactagata ttgtataatg ataacaatag ctattacaat taatattgga
     9661 caaaaataat gatactcaaa tatgtgattt gtattgatat gtatattata ttaagtcaac
     9721 tatccaatag agtaaaaggt ttttgtgaca tttgaagata ctctatagta tcttttttag
     9781 tgtaaaaact aagttttgtc ttttattaga taagtatctt ggatactagt ttatatttta
     9841 ttaagttgtt aaaaaagtgc acttaaaaag ttaaaaaaga aaaattaaaa aacctattta
     9901 tagaaactgt gaagtatatc ttaaacttgg gcacttaaaa gatatatgga gttagtagtg
     9961 caacctgcta taaatataaa taggagtaac ttttaattgt caaaaatata taaaatacat
    10021 aaataagtaa ttagtagaag atagatataa atatattttt gaaatgctcc ctacattcat
    10081 aacgagtgta gagtggcaga tacctgccca aaactccttt aattgctgga agttcctaaa
    10141 gctaactaaa ccacaacata atcatgaaat aaggataagt gtgatagagt gtgtatttaa
    10201 taagcactta aggttacgaa agtagaaaaa attagttaga tagcataagg ttaaatccta
    10261 agtgccttga aaatggataa tcagcaggta agcttcgaaa agaagaaact tcaacgacta
    10321 gagagaaatc tcgtacctac aagtgtgggg aaatggggag tacctaattt atggttaggt
    10381 aatgatatag tctgtgctta atagaaatat taagaagttc ataggagaac tggttaggag
    10441 taacgcccct aattgaacac actaaagata tattgttgaa taagattcat ataatattta
    10501 attattaaat gaatacaata tatgttttta gtatcaggag aattttgaaa acaaaaatta
    10561 aaaaatcaag tataatcagt gtggtgattg cttttagtat ggtatttact gcatttacac
    10621 caatagtatc ttatgctgat gaagtaatag gcaatgatac aataattaat ggagagaaac
    10681 cattagaagg tcagactcca gaaggagaaa aaccattaga aggtcagatt ccagaaggag
    10741 aaaaaccatt agaaggtcag attccagaag gagaaaaacc gttagaaggt cagactccag
    10801 aaggagaaaa accattagaa ggtcagactc cagaaggaga aaaaccatta gagggtcaga
    10861 ctccaaaagg agaaaaacca ttagaaggtc agactccaga aggagaaaaa ccattagagg
    10921 gtcagactcc agagggagaa aaatcattag aagaagaaat agttatagaa gaaatgagtc
    10981 taaagcaaga tattgataaa attcttgata tgactttatc tcaaataaat aaaatagtat
    11041 ataacttttg ggaagatgaa gaaaatgtaa agtcagatga acaatcagaa attaaccaag
    11101 tctttacttc aaaagactca tttatcagct tatggtatga taaaaaagca aaagtaaaaa
    11161 atacttgttt actaaaagaa atagatggtt cagacagacc ttatcatgat ttaagatttg
    11221 atgatattac aaagacttta acttttgact atgtaataaa aggtcttgta ggagcaagct
    11281 caaaagataa caaatacgta atagaaggtg aagatggaga gcaaactgca ttttgctaca
    11341 ataaccattt aagaccacca agtagtaatg gaaagagtcc atatttacca gcagaagaat
    11401 ttagtggtca agaaaaccag aatgaagatg cagttaaatc tatactatat gcaggttctg
    11461 aatttgatgg atttggatat aagcaacaat ttaatttagg cggagaagaa aatgacataa
    11521 tgacttactc agccactcaa agtgcaattt ggattattct tggacaagta gatgaacaag
    11581 aaaaacttaa gcagtatcaa ggctctataa acttttgtga ttttcttata gaaaatgcaa
    11641 aaaatgaaga agaaaaagct gagtatcaaa agaagaaaga agttgcgata aatacaaaag
    11701 cttatttaga agcattgtta aaagcaggtc gtgaagagtt aaagccgaat gatacaggaa
    11761 aacctagttt aagtaatggt ttaactaaga ttaactttga aaaaaatgaa gatggtactt
    11821 atgaaacaga agctgtagcc ttagtaggtt atagtggtgt agtaaagtta agactaccag
    11881 atggagtaac tgcttatgat ggagatggta atataatagg tacaggtgaa gtagaaattt
    11941 caacacaaca gaaatttaaa cttaagagtg ttggaaaacc agatgccaaa gctaatattt
    12001 ctgctatatc ttatgattat atttttccaa aagctattca atattataag gcagtaatcg
    12061 atttaggaca aaaagaccac tcatcttcat tgccatctag taagcaaaat ttattaagtt
    12121 atacagtaga gaaaaagaat ggccaagaag ttaactttaa cataagtcta ccaacagatg
    12181 atgttgtaaa tccaccagtg ccaccaactg atgatacagt aataaatcca ccggtgccac
    12241 caacagataa tacaaatgga cataagccta aaccttcacc accaatagat gatacagtaa
    12301 taaatccacc agtaccacca actgatgata cagtaataaa tccaccagtg ccaccaacgg
    12361 atgatgtagt gctaaatcca ccagtaccac caacagatga tgcagtgcta aatccaccag
    12421 tgccaccaac agatgatgca gtgttaaatc caccagcacc accagtaagt gatacagtgg
    12481 aaaaaatgcc agaactatca aaatatgaca caatagtaaa atcaccaaaa acaggtgatg
    12541 aaactcagct aatgtcatat gtatttatta gtatcattgc catttgtgga ttagcttatc
    12601 aatttaaaat aaaaagaaat taatctagat tattattaag tttaaaataa aaagtctcaa
    12661 attgatttat aagtcatttt gggacttttt tatgtaaata taatatgtaa atatagcttg
    12721 tttaaaagta tctattttaa ttatattctt aaacttatga ttagatatag gtattcatta
    12781 gttgtttgat gaatagaaaa atgttttgaa ggtgaaaata gtattgaaaa aataatatac
    12841 taagggtata attcatttga tttcatttgt ataaatttaa ttat
//
LOCUS       DAMJVM010000100        12833 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.104, whole genome shotgun sequence.
ACCESSION   DAMJVM010000100 DAMJVM010000000
VERSION     DAMJVM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12833
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.104"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            104..757
                     /locus_tag="PC523_002912"
     CDS             104..757
                     /locus_tag="PC523_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HDF2476415.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCILLNKQENNVCNIKA
                     IRNFSEIYSISRKSRYHSLLRLTEKGIIKKMGNTYEILNYDELHRLAFEVKEFLEDE"
     gene            complement(897..2273)
                     /locus_tag="PC523_002913"
     CDS             complement(897..2273)
                     /locus_tag="PC523_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2476416.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVGNIRKVTGLTCMLA
                     IILSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAISTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYFYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAVGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(2289..4202)
                     /locus_tag="PC523_002914"
     CDS             complement(2289..4202)
                     /locus_tag="PC523_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2476417.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIDMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELKPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLELEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            4338..4970
                     /locus_tag="PC523_002915"
     CDS             4338..4970
                     /locus_tag="PC523_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDF2476418.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            5109..5648
                     /locus_tag="PC523_002916"
     CDS             5109..5648
                     /locus_tag="PC523_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF2476419.1"
                     /translation="MIFVEELWEKAIEFHGHECPGLAIGFKAALAAREYLGCKQSGDE
                     EIVCISENDACGVDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FEMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(5814..5984)
                     /locus_tag="PC523_002917"
     CDS             complement(5814..5984)
                     /locus_tag="PC523_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2476420.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDASSCIDCGSC
                     AGACPVDAPQPE"
     gene            6269..7336
                     /locus_tag="PC523_002918"
     CDS             6269..7336
                     /locus_tag="PC523_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HDF2476421.1"
                     /translation="MKNKLIYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFKKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSVANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVVGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPHIIPTSITSSSSRNNYQPKVL
                     DGSEKDRVLNKLEKISKDLNQ"
     gene            7506..9407
                     /gene="cooS"
                     /locus_tag="PC523_002919"
     CDS             7506..9407
                     /gene="cooS"
                     /locus_tag="PC523_002919"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HDF2476422.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVIKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFRLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            9400..9873
                     /locus_tag="PC523_002920"
     CDS             9400..9873
                     /locus_tag="PC523_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2476423.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            9848..11074
                     /locus_tag="PC523_002921"
     CDS             9848..11074
                     /locus_tag="PC523_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2476424.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENIYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVNWIAGKTVKGIDVNKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLREGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIILDDSKNVK
                     GVAFSNGEILDADMVIVATGVKPNADFLDETGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMAGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGLNDYKKFIFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDVCY
                     ADFFKIKENGEFCYSV"
     gene            complement(11227..12414)
                     /locus_tag="PC523_002922"
     CDS             complement(11227..12414)
                     /locus_tag="PC523_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2476425.1"
                     /translation="MNITKNFLKDYLERFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            12687..12782
                     /locus_tag="PC523_002923"
                     /pseudo
     CDS             12687..12782
                     /locus_tag="PC523_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         4836 a         1597 c         2063 g         4337 t
ORIGIN      
        1 caagtttaat ttaactataa tcatatcttt tattaaaaat aatataaatt aaaacatata
       61 aataaaatat tttatacaat atattgtaaa ggaggtaact ttgatgaaag cagaaaaaac
      121 aagtatatat ccaattatta aaaataattt aaacaaaata tcaattgttg aatataaaaa
      181 gggtcaaaga tttgtagcat cagataaaga cttgcaagaa gtattcttta taatagaggg
      241 agttgcatta gtggagtgca ctactagagg agggaataaa tttcttgttg acattgtacc
      301 tgaaaatgaa tttataggca aaatcagtta tatatatgaa cataatttga aatgtgatat
      361 atttgcaaaa accaatataa aattatttcg atttgaaaaa aatgtatttg aagagtttta
      421 tcttaaacca gattttattg cactatttca cagaaaatgt acaagaagaa tatatgaatt
      481 atataaaact agaatggtaa gagaactatt ttcttgtaca gaggttattg cctattgtat
      541 attattaaat aaacaagaaa ataatgtctg caatataaaa gccattcgta atttcagtga
      601 aatctattca attagtagaa aaagtagata ccattctctg ttgaggttaa ctgaaaaagg
      661 tataataaaa aaaatgggta atacatatga aattttaaat tatgatgaat tacatagatt
      721 ggcatttgaa gtaaaagagt ttttggagga tgaataaaga ttattactta atcaaaataa
      781 ggtataataa tttctgtaaa aaatagaaat agtctcactg aaaatcaaaa atactttagt
      841 tgttttcaat gagactgttt agaaatattg tatacttaca atgtatagta tagttattag
      901 tcaatttcct ttactatgtt aatagcccat tttttagata atgctctagg aacaccaaat
      961 ataaacttaa caacttcttc actgtaagtg agtagaacaa gtacaaatat aggtgcacct
     1021 ttaaatgcag ctataaaagt tagaggaata gaagctagcc acatacatcc taattctaaa
     1081 aatagagcat atttagtatc tcctccactt cttaaaatac caattataac caatgtatta
     1141 aaagatttga gagccattag tattcccatt ataaagaata tcttagttat atcagaagct
     1201 aagctatcag aaacactaaa catttttaat aataatggta tatttaaaat taaaataatt
     1261 ccaagtatta aaccagctga aaatacaagt atagaaaact ttttagcata ttcaatggct
     1321 cttttaatat ggtcagctcc aagttcattt ccaagcataa tagatgcacc agatgcgata
     1381 catacagcag tcattatgaa gaaattgcct gtactggttg ctatctggct ggctgctata
     1441 gcagaagtac cagctcttgc atatgcaaca gaatacagca cagttccaac tgcccataca
     1501 gagtcattta caaatatagg aaagcttttt gatgaaaaag attttataaa aattttatca
     1561 atagctttta gatttttcag accaaatttt aatatatagt cttttttata aaagtaaaca
     1621 taagtaatca ttaaaattaa ttcacatatt ctagcaataa cagtagctag agcagcacct
     1681 tttacaccca gtgcaggaag accgaagtta ccaaagataa atccataatt taaaataaca
     1741 tttgttacaa gtgcaaatgc actacaaatc atacctaact taggatttct aacacctctt
     1801 gaaccagtac taaatacagt acttatagca attaatggat aactaaatac tgcaatacta
     1861 aagtaatcta tacaaagttt tacaacttca gaatcatacg aaaatatgtg tataattggc
     1921 tttggtgtta gtaaagcagg aataacaaat acaaaactta aaataatggc taacatacaa
     1981 gtaagacctg taacttttct tatattgcca acatccttct ttccataaaa ctgtgaaata
     2041 aaaactccag cacctcctgt gatgccagat aaggacatat taaaaagaaa gaaaaattgg
     2101 tttgcaactc caactgaagc tacagatgtt tctcctaaac tacttatcat aattgtatct
     2161 attacattta cagaagtaga aattaaattc tgtataataa taggtataca taaagataga
     2221 agtattttat aaaatctttt attttcgtct tttaaagaaa atagttcttg caaataatcc
     2281 tctccttttt acataaattc ttcccactca tcatatagag aggcaaggtt attttctaaa
     2341 ttaatttttt cttgactaac ttctttagcc ttatctggat tagagtatac ttcttcttga
     2401 cataaaagta tatctaattt ttcaatttca agctctaact tttcaatttc tttttctata
     2461 ttttgtcttt ttattctatt tttcttttct atttctcttt gttctctctc ttttcttttt
     2521 tcatctttta tttgagttct agttttttct tctttttctt ctataatact catttcttgg
     2581 atttgccttt ttttatctat ataataatca taatttccta gatattcagt aattccattt
     2641 tcatctaaaa ctaaaatttt atcaacaaca gtatttagaa agtatctatc atgtgatatt
     2701 gtaaatatag ttccatcata attagttaat gcctcttcaa ggacttcctt agaatcaata
     2761 tctaggtgat tagtaggctc atctaataat aataagtttg catttgatag tataagttta
     2821 agtattgcaa ctctagctct ttctccaccg ctcaatgtag aaattttctt aaaaacttct
     2881 tcatctacaa ataaaaatgc tcctaacata tttctcaaag tagtttgagt aagatgagta
     2941 ttattttccc aaatttcatc aattattgta ttatctaaat taagagtttt ttgttcctga
     3001 tggaagtagg aaacatggac attagttcca aatttaatat tgccactaag aggtttcagt
     3061 tcattagtta ttattttaaa taatgtagat ttaccaacac catttgcacc tataagagca
     3121 actttttccc ctctatatat atctaaatcc aagtctttaa aaagtattct ttctccataa
     3181 cccatagata catctctaat ttgaagaaca tcattaccac tctggacaga aggcgtaaat
     3241 tgtatacgag ctttttttct ataagcttct ggtttatcta aaacgtctac cttgtctaat
     3301 gctttttctt tacttcttgc acgctttagg tgtttttctc taccataagc ttttagtctt
     3361 tcaattgatt cttcttgctt ttttatttct ttttgttggt cttcaaattt cttaagttct
     3421 agttcttttt ctattttaga taactcaaca aatttagagt agttaccatt atatacttta
     3481 agttttttgt tatgaacttc aaaaattcta tttacagatt ggtctaagaa atatctatca
     3541 tgagatatta acatgacagt ccctttatat tgttttaaaa atacttctaa ccattctatt
     3601 gcttcagagt ctaagtggtt agtaggttca tcaagtaaca atagagttgg tttcttaaga
     3661 agtaattttc ctaaaagaac tctagttttt tcaccaccag aaagtatact tattggctta
     3721 tccatatcat tgtcactaaa tcctaatcct tttagaactc ctttggcttc agacttatat
     3781 ccatatccat ttaaatctga aaataattcc agtttatgtg aatattcatc cataattttt
     3841 tctataattg gagaatttgt ttttgtactt tcttcagata ttttaacctc caattctctt
     3901 aaataggact ccatatctat caaattttta aaaacttcta gtacttcttc taaaatagta
     3961 ttatccgaat aaaaattggt attttgttct aaatatccga tttcacaatc tttagaagtg
     4021 taaatatctc cactatcata tccataaatt cctgaaatta ttttaaataa agtggttttt
     4081 ccagtaccat taacaccgat tataccaatt ttatcgcctt catttactgt aaaactaata
     4141 ttttctaata tagaatctat accaaaactt ttatttaagt tattacatga caaaacaatc
     4201 ataaatttct acctccttga actatctaaa attataatgt tttgatataa tataatcaag
     4261 gttatatgta ttagttttga aaaagaaata ttttattgtg aatcaaacaa aaaagaggac
     4321 acttttagga ggtgaagatg ttgggaaata aaaatatatc aatggcagtt ataagaaggc
     4381 taccaaaata tcatagatat cttggagact tattagatag ggatatacaa agaatatctt
     4441 ctaaagaatt gagtgatata atagggttta ccgcttctca aataagacaa gatttaaaca
     4501 actttggtgg atttggacaa caagggtatg gttataatgt agaagctctt catactgaga
     4561 taggtaaaat tcttgggttg gatcgaccat ataacgcagt tcttgtagga gcaggtaact
     4621 taggacaagc tatagccaat tatgcaggat ttagaaaagc tggattcgag ataaaagctt
     4681 tatttgatgc aaatcctaga atgataggtt taaagataag agagtttgaa gtattagatt
     4741 cagatacttt agaagacttt ataaaaaaca ataatataga tattgctgta ttatgtatac
     4801 ctaaaaatgg agcacaagaa gttattaata gagttgtaaa agctggaatc aaaggtgtat
     4861 ggaattttgc acctttagat ttagaagttc cgaaaggtgt tatagttgaa aatgtaaact
     4921 taacagaaag tttatttact ttatcgtatt taatgaaaga aggaaagtag acaacttaaa
     4981 catattaata atgagtagtt gtgaagagta ttgagaaagt cagaagattt aatattaaat
     5041 catctggctt tttgaaatat taggataaaa atttaaataa tatatgtaaa taaatagtat
     5101 taaggagagt gatttttgtg gaggaattat gggaaaaagc aattgaattt catggtcatg
     5161 aatgtccagg tttagctata ggatttaaag cagcacttgc agcaagagaa tatttaggat
     5221 gtaaacaatc aggagatgag gaaattgtgt gtatttctga aaatgatgct tgtggagtag
     5281 atggcataca agttgtatta agttgcacaa tgggtaaagg aaatttacta cttaggatga
     5341 ctggaaagtc tgtatatcat ttctttaata gagaaaatgg gaaatctata agaatatatg
     5401 taaagtcaaa accatttgag atggatagag aaggatataa gaaatattta ttagaaagtg
     5461 attttgaaga aatatttgag attacagata ctaagttaag acttcctgaa aaagcaagaa
     5521 tatttaaatc tcttaaatgt gaggtgtgtg gtgaatttgc atctgaacaa tatataagga
     5581 ttgaagaagg aaagaaagta tgtcttgatt gctttaataa atatgaaaga ttttatgagg
     5641 agtattaaaa ttataaatta aaagtattag atatgcgcta acaatatttt aagatgaatt
     5701 aggtagtaac tataataaaa tatataaata atattacttg aatcatacat aattaaaaag
     5761 aggctgatta gtatttagaa aataaaatca gtctctttat gaataacgaa ttattattct
     5821 ggttgaggtg catctacagg acaagctcct gcacaagaac cacagtctat acaagaacta
     5881 gcatctataa catatgcgtc gtctccagct gatatacagc taacagggca ttcagcctca
     5941 caagcaccac agcttataca agcgtcagta attttatatg ccattttgaa atcctccctt
     6001 ttatatgtat tgtaattcaa taacattata acaaatattg ttgaatataa caattaaatt
     6061 aattctacaa caaaaacttt attatgatat tcttgtttta gagttttaca ataaagttta
     6121 atctgcttat atttttattt attttaaaga gatttatttt aaaaataaaa ttaaatttaa
     6181 aaagttatgt attgattata tttgaaaatg ttatactcta aaaaagtata ttttatttta
     6241 aaatttatta atacaaagga gatttactat gaagaataaa ttaatttaca tatgtatcat
     6301 gagtctactt ctatttgtag taggatgttc taataatcca aacaaagaaa gtactattaa
     6361 aaaagactct aagaataacc aaagccaagt taaagaagaa aaaagagaac ctgtgaaaaa
     6421 agatataact ataagtttcg ctggtgatgt aactatggga aactataaag gctcgtcata
     6481 ttatggtagt tttgaccatg aatttaaaag acaaggagaa gattataatt attttcttaa
     6541 aaatgtaaaa cctgtattta aaaaagacga cttaacagtg gttaatttag aaggtccact
     6601 tactacagcg tcaaatgcaa aagaaaaaaa atttgcattt aaaggaaagc ctggatatgt
     6661 aaatatttta aaatctggaa gtgtagaggc agtgagtgtt gcaaataatc attcagaaga
     6721 ttattttgaa gaaggaatga aagatacaca gtttatattg gatgagaata aaataaatta
     6781 ttttggatta gggaaagatg ctgttgtaga tgtcaaagga attaaagtgg gtctattagg
     6841 gtataatggc ttatcaactg agtataatga aaaaaactta aaacaaatgg aagatgatat
     6901 taagtcctta aaaaagaaat ctgatgttgt tgtagtgtac tttcattggg ggatagaatt
     6961 agattattat ccagataaaa aacaaaaaga ttttgctcat tatgcaatag acaagggagc
     7021 tgatttagtt gtggggtctc acccacatgt gattcaagga atagaaaagt acaaaaataa
     7081 gtatatagct tatagtttag ggaatttttg ttttggaggg aacaagaatc cttctgatac
     7141 agatagttat atatatcaac aaacttttac tttttcagat aataaattaa cttcaatcaa
     7201 agagccacat ataataccaa catcaataac atctagtagt tctagaaata attatcaacc
     7261 aaaagtattg gatggaagtg agaaagatag agttttgaat aaactcgaaa aaataagtaa
     7321 agatttaaat caataaaata taaaaaaaaa taaatatata tatttatttt aataatttat
     7381 attctgttta ggatatggga aatcgtggta ttaaataagt agattttaat agttaaatta
     7441 tagtcaatta taataaagct atttaatcta cttttttaat ataaaaatat aagggggaat
     7501 ttgtaatggc ttgtaacact tgtaaaatgt gcgaaagtgc agacaagaaa ttagaaagtt
     7561 ttgtagcttc aaaggatgtt gaaacagcat ttcatagaac agaagatcag aaagttaagt
     7621 gtggttttgg acttcagggg gtttgttgta gactctgttc aaatggacca tgtagagtaa
     7681 ctcctaaatc accaagaggt atatgtggag cagatgcaga tactatagta gcaagaaact
     7741 ttttaagagc agtagcatca ggagcagctt gttatttaca tgtggtagaa aatacagcta
     7801 agaatttaaa aaatgttgga ataactaaag gtgttattaa aggagaaaaa acattaaatc
     7861 aattagcaga gatgtttggg atagaaagtg atgaaaagta tgataaatgt ataaaggttg
     7921 cagacaaagt tatcaacgat ttgtataaat cgagagatga taaaatggaa ttagtagaaa
     7981 aaatagctta tgcacctaga gtgaaaaagt ggaaagagtt aggtataatg cctggtggtg
     8041 caaagtcaga agtttttgat gcaatagtaa aatcatcaac aaatttaaat agtgacccag
     8101 tagacatgct agttaattgt ttgaacttag gtatatcaac aggattatat ggtcttacac
     8161 taactaatct tttaaatgat gttatgttgg gagaaccagt tataagaatg gctcctgtag
     8221 gatttaatgt aatagaccca gactacataa atataatgat aacaggtcat caacattcaa
     8281 catttgcaaa tttccaagat agattaaaag atgaagatgt tataaaatta gctcaatctg
     8341 ttggtgcaaa aggatttagg ctagtaggat gtacatgcgt tggtcaagat ttacaattaa
     8401 gaggagaaca ttatcaagaa gtatttgctg gacatgcagg taacaatttt acaagtgagg
     8461 ctgttttatc aactggagca atagatatag ttctttcaga atttaactgt actataccag
     8521 ggcttgaacc aatagcagat aaatataaag taaaaatggt ttgtttagat gatgttgcta
     8581 aaaaagcgaa tgcagaatac ataggtttag acagaagtaa attagatgaa cttagtaaca
     8641 ctttaataga aaaagcattg gagtcttata aggagagaag aggaagtatt gaaatagata
     8701 taccaaaaga ccatggattt gaacaatctt tgactggtgt aagtgaaaag aacttgaaag
     8761 cgtttttagg agattcatgg aaacctctta ttaatttaat tgcagaagga aaaattaaag
     8821 gtgttgcagc tgttgtggga tgttcaaata tgacagcagg aggacatgat gtaaatactg
     8881 ttgagcttac taaagaattg attaaaaaag atataatagt actatctgca ggatgttcta
     8941 ctggaggatt agaaaatgtt ggtcttatgt cacctggagc agaagaattg gctggggaaa
     9001 acttaaaaga agtatgtaag actttaggaa taccaccagt attaaacttt ggaccatgtc
     9061 tagctatagg aagactagaa atagtagcaa cagaattagc agctgagctt ggaattgact
     9121 taccacaatt accactagtg ctttcagctc cacaatggtt ggaagaacaa gcattagctg
     9181 atggagcttt tggtcttgct cttggtttac ctcttcattt agctttacct ccatttataa
     9241 caggaggaaa attagtaaca gaagtattaa cagaaaaatt aaaggactta acaggaggac
     9301 atgttatagt gaatccagac cctaaatctt cagctaatca attagaagaa ataataatag
     9361 acagaagaaa aaatttaggg ttaaaggatg tggaatgtaa tgcatagaat ttttataaat
     9421 aaagatttat gtacaggatg taagagctgt gtactagcat gtatgctaaa acacaataaa
     9481 gattatgaca tgtacactct tgatttagaa aatatagata atgatagtag agggcatata
     9541 gaattagata gtaaacataa taatccagtt ccaattcttt gtagacactg tgatgaacct
     9601 gaatgtgtat tagcatgtat gagtggtgca atgcataaag atagtgaaag tggaatcgtt
     9661 tcatatgatg aagagaaatg tggttcatgt tatatgtgtg taatgtcttg tccatatgga
     9721 ttattaaagc cagatgatag aagcaagcaa aacatactta aatgtgattt atgtaaagat
     9781 gaggagtatc ctagatgtgt agctaattgt ccaagtggag caattgaatt acagaaggag
     9841 gaaaatgatg aattatgtag tgttaggggc tagtgcggct ggtataaatg cagtaaaaac
     9901 attaagagaa ttagataaag attcaaatat agtagtaatt tctaaagatg aaaacatata
     9961 ctcaagatgt atgttgcatc atgttatatc tgaacacaga actttaaaac aaataaattt
    10021 tgttgatgaa gattttatgg agcaaaataa tgtaaattgg attgcaggca agactgttaa
    10081 aggtatagac gtaaataaaa aagtagttca gacagaagac attactgtaa attatgataa
    10141 acttttaata gctacaggtg catcttctgc tattcctcct ataaaaaatt taagagaagg
    10201 aaattttgta tattctgtga gaaacataga tgacatttat aaaataaaag aaaaagcaca
    10261 aaattctaaa aatgtagtta taataggtgc tggtctagtt ggtatagatg cactagtggg
    10321 attatttaaa tatgagcagt tgaatatttc tgtagctttt atggaaaagt acattttaga
    10381 tagacaattg gatgaatata cagcatcagt gtatgaaaat aagttcaaag aaaaaggtgt
    10441 aaaattttat ccaagtgcat caatacaaga aatcatattg gatgacagta aaaatgttaa
    10501 aggtgttgca ttttcaaatg gagaaatttt agatgcagac atggtaattg tagctacagg
    10561 ggttaaacca aatgctgact ttttagatga aacaggaata gaatatgata gaggtataat
    10621 aatagatgat atgtgtcaaa caacacaaaa agacatatat gcagcaggag atgttgtagg
    10681 taaaaatgct atttggcctc tagcagtaaa gcaaggaatt gtagctgcat ataatatggc
    10741 tggaaaagat aagaagatag aagatgaatt tgcttttaaa aatagtatga atttcatgga
    10801 tataccaact atttctatag gaatgaatac acctgtagat gatagctata aagtattaac
    10861 cagacatgga cttaatgatt ataaaaagtt tatttttaaa gataatgtaa tctatggagc
    10921 agttatacaa ggagatattt catatgtagg tgtattaact tatttaataa aaaataaagt
    10981 tgaaatatat gatttagaaa acaggatatt tgatgtatgt tatgcagact tttttaagat
    11041 aaaagaaaat ggagaatttt gttatagcgt ataatatata tggagtaagg tttataaata
    11101 cagtaaatca ataaatacag taaatcaata aatagagtta ttctaaaata agaaaaatga
    11161 gaaatcttat ttagaaatag ctctattttt gtaattaagt ttaaattata tttaatttgt
    11221 aaaatattat tttgaacaat tatacatata ttctctagtt agaggtaaat cattttttat
    11281 gcctttagtg aaaagtattt gatgtatatc tataatacca gtattgaaag cagcggcaca
    11341 agaacaaagg tataaatccc acattcttat aaacttatca tcaaacattt ctttaatctt
    11401 tggaagattc tcagagaagt ttttttgcca gtatagtaaa gtttttacat aatgtaatct
    11461 tagactttcc acatctatag tatgatactt gtaatcacta caaatatgta tcatttctct
    11521 tagactagga ataactcctc ctgggaaaat atattttttt atccaagggt ctccataaga
    11581 ttcctgcaac ccacttatat agtgaagtaa aaataatccg ccatctttta aaacagcgtt
    11641 tacgttttta atgaaaagtt catagttttc tctaccaaca tgttcaagca tcccaacgct
    11701 tacaacccta tcaaacataa ggtctgattt ttttaattct ctgtagtcca taagtcttac
    11761 ctcaaggtag tcttgaaggt tctcttcttt gattcttgcg ttaaattcct taaattgctc
    11821 ttcgctaagt gtaattccaa caccatgaat tttatatttt tttgcagctt ctattagtaa
    11881 aaatccccaa ccacaaccaa tatcgagaag actcatacct tcctctagat ttagcttagt
    11941 taatatgtgg tgaactttat tgacttgtgc atcataaaga gtatcattat catttctgaa
    12001 gtatgcacaa gaataactaa gtgtcttatc tagccataag ctataaaaat cgttaccaat
    12061 atcatagtgg gcatgtattt cttttttctg atgtctcatt gaatttgatg agtgtaatag
    12121 atgttttaca gctgaattat caatattgaa tttgtctatt tgttttaaaa gtagatttaa
    12181 agttaagaat aaatctccct caacttcaat atctctattt atgtaggctt ctccaagtgc
    12241 taaagatgta ctttttaata gttcagactt gctaatatcg ttatttacta taactttgaa
    12301 ctgaggggag ccttctccaa ttagaaattc ttcctcatct ttaagcctaa ctagaaatgg
    12361 tgtttcacca aatctctcta aatagtcttt taaaaaattt tttgtaatat tcatattacc
    12421 agctccttct aatgtaaatc cagtaagttt ccctgcttaa aatatattat agtccttttt
    12481 ttaaaaaagt aaaatttttt caataaattt tttatttaaa ggaaatttat tttttataaa
    12541 gaaatttcat gttgtagtat attttttgcc tataaatttt ggtactggat ataatattaa
    12601 actgttaatg atataataaa tttaatctat aaagtaaata aaattttgta ataaatagtt
    12661 aaaactattt tataaggaga tataggatgg aagtatatgc tttggttggg tcaagtggca
    12721 caggaaagag ctttaaagct ttagagttcg cgtatgaaaa tgatatagaa tatataattg
    12781 attaaaaaat agctacgcac actcgtgact ttagtcatga attagtagct aaa
//
LOCUS       DAMJVM010000101        12705 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.105, whole genome shotgun sequence.
ACCESSION   DAMJVM010000101 DAMJVM010000000
VERSION     DAMJVM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12705
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.105"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            331..1266
                     /locus_tag="PC523_002924"
     CDS             331..1266
                     /locus_tag="PC523_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDF2476426.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSDDPNGFWIILLISAIICIVITFFMSRNKLL"
     gene            complement(1358..3229)
                     /locus_tag="PC523_002925"
     CDS             complement(1358..3229)
                     /locus_tag="PC523_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDF2476427.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKIESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFIRCEKWLKSAADNNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNVEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     AKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEDFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDN"
     gene            3422..4348
                     /locus_tag="PC523_002926"
     CDS             3422..4348
                     /locus_tag="PC523_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDF2476428.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTNKSFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLLLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            4487..4633
                     /locus_tag="PC523_002927"
     CDS             4487..4633
                     /locus_tag="PC523_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476429.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(4738..5262)
                     /locus_tag="PC523_002928"
     CDS             complement(4738..5262)
                     /locus_tag="PC523_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2476430.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     ESMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLARVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(5315..5539)
                     /locus_tag="PC523_002929"
     CDS             complement(5315..5539)
                     /locus_tag="PC523_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476431.1"
                     /translation="MIIKLTKDDFNFRFKQYEGMFFHAEIIESMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQIEAYIDLKSIK"
     gene            5736..6860
                     /locus_tag="PC523_002930"
     CDS             5736..6860
                     /locus_tag="PC523_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HDF2476432.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     VLSKVSGIFPFSLYEITMYIIVISIFLFLCCTLSTIFNKKKKLNIFLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLENNTSIQSKQHSVKELTKLYKFLIIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPSVSGSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESKFWQKYEGKID
                     EISNEFNNSYLKANGVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(7334..8158)
                     /locus_tag="PC523_002931"
     CDS             complement(7334..8158)
                     /locus_tag="PC523_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476433.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTSVYLASTVS
                     TITKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(8506..9561)
                     /gene="ispG"
                     /locus_tag="PC523_002932"
                     /gene_synonym="gcpE"
     CDS             complement(8506..9561)
                     /gene="ispG"
                     /locus_tag="PC523_002932"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDF2476434.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            complement(9744..10748)
                     /gene="rseP"
                     /locus_tag="PC523_002933"
     CDS             complement(9744..10748)
                     /gene="rseP"
                     /locus_tag="PC523_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDF2476435.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFWGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            complement(10761..11915)
                     /locus_tag="PC523_002934"
     CDS             complement(10761..11915)
                     /locus_tag="PC523_002934"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDF2476436.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELVNITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYADSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            complement(12079..12282)
                     /locus_tag="PC523_002935"
     CDS             complement(12079..12282)
                     /locus_tag="PC523_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2476437.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
BASE COUNT         4263 a         2116 c         1331 g         4995 t
ORIGIN      
        1 aaattaaata taacctatat attttatatg taaattatga taaaaaaatt atttaatcaa
       61 atcaaaactt gatttatata cctggaaaag gtattatatt aaagagtgct taaaatacgc
      121 atttcataaa taactatata aaaaataata aataaggaga gtgatgggca atggatacta
      181 gtccgttgcg gagagaattt tttacattct catcacttaa taatatctaa aatcaaaact
      241 aatacatata ttcagttata taaattagtt aattaataga tactattaaa tgatgagatt
      301 tatataaaac tgaataaagg atgtgttgcc atgataagat attacaaaac tatagattca
      361 aaattagaga aacttagttt ttttgaagat ggctgttgga taaatcttgt tgaacctaat
      421 catagtgaaa tcaatgaaat ttctaattta ttaaatatag atgtagaaag catagaatct
      481 gcactagatg aagaagaacg ctcaagaata gatgttgaag acaatcatac tcttatactt
      541 atagatatac cagtagacga aagtgactct aattcttctc attatactac aataccctta
      601 ggtattatac tgactgaaga agctattgta acagtttgtg atgcacaaac taagatttta
      661 aacgacttta tagttggtca tattaaagat ttttttacat ttaaaaagac tcgcttttta
      721 cttcaaattc ttcataaaaa cgcagcttat tacttgcatt acttaagaaa aataaataaa
      781 atgacaatca taatagagcg agaaatttac aaatcaatga aaaacaaaga attggttcaa
      841 ttattagaat tagaaaaatc tttggtatac ttctctacat cactaaaatc taatgaactt
      901 gttttaaaca aaatggttag aactgctggt ataaagaaat atccagatga tgaagaccta
      961 ttggaagatg ttatagttga aaatagacag gctcttgata tggctaaaat atacggagat
     1021 atattgagta gaattatgga tgctttcagt gcaattataa gtaataatca gaataatgtt
     1081 atgcaaattt taacggtagt tacactaatt ttctcaatac ctacaataat atcaggattc
     1141 tttggtatga atgtcatcaa catgccgttt tctgatgacc caaatggatt ttggataata
     1201 cttcttattt ccgctataat atgtatagta attacatttt tcatgtcaag aaacaagcta
     1261 ttataaaata ttaataaaaa agccattaga actaattgat tttcatttat cctaatggct
     1321 tttttatatt taaatatata taaccttata atacttttta attatcatgt tcttcatcta
     1381 taatcatttc agtttcaagc ccttctagca ttgaatctaa actttctttt atatttagta
     1441 tataaattgg tacatactct tcttcatcta ttacctcttc ttgcaactta aatggaggaa
     1501 ttaatacttc atcaaagtct atatttaaat taggaaagtc ctctactatc tcttcacaat
     1561 tatcgtattc ttcatttgct aaagaaatgt acatatctag gctcttttta taataaagac
     1621 ctgcaagcat gtactcacac ttcttacttc catatttaat accttcttta taatatttct
     1681 cagcttctgc gtcatcattt aattttaaat aaatttgacc taaattatat attgattcaa
     1741 cacaaccttg tgccctagcc ttttcatacc aaaatttaga atttacatag tctttataaa
     1801 acatatcata aattaatccc accatatgtt gagcatacac attattttca ttggcaggag
     1861 tatcaaacat cattttagcc tcttccaatt taccttctct aaaataaatt ctacctagat
     1921 gtatacgagc attcatatgg tttttgctac ttgccatttc aaaatacttt ctagcatttt
     1981 ttgtatttcc taaactctca taaatacatc ccaatctata actagacctc gtatccccat
     2041 tttcagatgc tttcttatac caacttatgg cttcttcaac attatttaaa tcttcataga
     2101 tatttcctaa tttgatttga caagatacat tatttggaat ccttgaataa tacgaaatcg
     2161 ctttttctat atcttgattc tgataatata aatcccctag attctcaagg gaacttttgc
     2221 atccaacagc tatagcatcc tcataatatt taatagcatt ttcataatct ttttcttcaa
     2281 aatatatacc tgccagatta ttttttgctt ctgttatatc atcttcagcc gccattttat
     2341 aataagtttt tgctttttct atctcatttt ctctattgta taaattagca agtctatact
     2401 tagaaagtgt atgatttttt tctattgctt tctcataata ataaattgca tctcccagtt
     2461 cttcttttct atcatataca tatgcaagtc tatactcaga cttcacatca tctctatcag
     2521 cactcttttt ataatatttt tctgcatctt ctaaattatc atttatctcc ataatcatac
     2581 ccaagttaaa gcaacctttc atatgccctg acttgtacaa ctgtttatat aaactcactg
     2641 ttgatttatt atctttcttt tttccattta atattgctaa attatatttc gcatcatcat
     2701 ctaaaagcac agaacctttt ttataatatt catatgcttc atcatctttc tccaaaaaag
     2761 tatataatac ccctaaatta aaacaagctt tacctaaatt attgtctgct gctgatttta
     2821 accatttttc acatcttata aaatctttca ttttaaaatg gcatacacct aaattatttt
     2881 gtgcttgcaa ataacctaaa tttgctatct tttcatacca atatatagca tattcataac
     2941 catctaactt atagtacata ttacctaaat caaaagcagc atcttcactt ccacttttat
     3001 atgccattga ataccacttt tctgcttctt taaattgttt tttactttca tatatatttc
     3061 ctaaagtata ttgagatttt acataattat ccaatgcagc actcttatac aactctattg
     3121 cgctttctat cttacccatc ttttcaagga gggcaccctt agtatgtacg actttcgggt
     3181 cattagatat attttttatt ttttttatag aaaattttgt atttttcatt attcgctacc
     3241 tacccatttt ataaaatata ttctctcaaa cattattata cattataatc tataattaat
     3301 gctataatat tgaattttta ccttttgtca ctatattgta acattaaatt ttaaatttta
     3361 taatcattag attttattat gtaattggta tataatgtat atataagggg agggacattt
     3421 tatgaatttc agcaatattt ttattcaagt tgcagtttta tttataatta ttttagttgg
     3481 gtattttgtc agaaaattca acttattaga tgaccactgc acatctaagc tttcaacttt
     3541 gacaatgaca gtttttttac cctcaatgat aattagctcc atgcaaatta actttgatag
     3601 taaaatgata caaaaaatac tattgttatt atttacatct ttgattatgt atatagtttc
     3661 aattataatt gcatttttgt taaaatacat tttaaaatgt aatgataaaa aagatttggg
     3721 aatatatcag tatatagttg ttttttcaaa tgttgctttt atgggttatc ctgtaattga
     3781 agcagttcta ggtcatgaag caatattcta tactgcaata ttcaatttgc cttttaactt
     3841 attttctttc actttaggaa tttatctttt atctaaaggc agtacaaata aaagtttttc
     3901 aataaaatca cttataagcc ccgctacaat agctattgtt attggacttt ttttatttgt
     3961 aacaggactt aggttacccc aatttataaa cgagccactt gagatgttgg gaagtataac
     4021 tacaccaata tccatgatta ttattggaag tttacttgct aattcttcag ctctcgattg
     4081 ttttgtcaac aaaaaattat atatagtcac ttttataaga ttattgctgc ttcctgtgat
     4141 tgtatatttt attctaaaag gatggataaa tgataagatg attttggcta taccagtagt
     4201 aatttcttca atgcctgctg ctgcaaatac tgctataatg gctaatcaat atgactccaa
     4261 tataacacta gcttcacaat gtgtgttttt tacaacacta ttttcagtta tatctatacc
     4321 ttttataagt attttactct tatcttgatt tattttttta tgtattatat aataacatat
     4381 tcaataaatt acaaatataa acattttatt gtagtatatt gatggaattt tttcctagat
     4441 atttgtcaat aatatctata tgtattatag aataggaggc ttaaatatgg atatggattt
     4501 actctttaaa atagtatttg ctatagttgc tataggtgtt tctgtaaaaa tcatcaaaac
     4561 tgttgctagt ttagctttta aaatagcgtt gatattgcta ataattttat ttgtatatag
     4621 aatgtttttg taaacattta aaaataagtt ttttaataaa atagagaagt aaaatagatt
     4681 atctttaatg taaatttttt attaaagaca ctctatttta cttctctatt agttttttta
     4741 aattaaattt ttattatatc ttcccataat ataaacatct tcaaattttc catctctgat
     4801 tacagcatat tttttagttc cctctatttc aaaacctagt tttttatata aatttaatcc
     4861 cttttcatta tccacaatta cttctaatcc tactctagct aacataatcc aattatctgc
     4921 tatgtctaaa agttgttcca taagcttttt acctactcct cttccctgat aattctctgc
     4981 aacacttatt cctatttctg cactatgtct caatcgtgga gaagaattta catgtaaccc
     5041 agcaaagcct attacatctt tttctccatt attattagtt tcagctacaa acatataatc
     5101 attttctccc aaagaagcta acatagattc agttttatcg actctgtcac tcattaatgc
     5161 caaagtattt ctcataacac tgtatgttct tctaatttca ttaaaagctt gtgcatcttc
     5221 tcttcttata ggtcttattg ttatatctat aggcttactc atattggatt ccccctcaaa
     5281 cataatcttt atttttaatt aaaattttac atctttattt tatacttttc aaatctatgt
     5341 atgcttctat ctgaggtaca tatatcatat tatcttttat atgtgtactc ccaaattctt
     5401 ttaattctgt accttcttta tccttagtta ttttatatct gatggattcc atactttcaa
     5461 ttatttcagc atgaaaaaac ataccttcat attgtttaaa tctaaaatta aaatcgtctt
     5521 tagtaagttt tattatcata tttttatcta ctccttatat aattaatatt tttcttatta
     5581 ttagttgtat acatattgct tattcatatt aatatattat aacttatcat ttatttagta
     5641 taaagttata aatagatata tttatactta ttttacaatc tttcttcaat tgatatataa
     5701 tatgtttata taaactaatg tatgtgaggt gaaatatgag caaaaaaaca aaatattttt
     5761 ctttaattct ctttcctatt gctttgcttt taaattttat agccagcaaa gttccaaata
     5821 ttgttgaaaa atattactcc caatttatag ataaaataat tgtacaagta ttgagtaaag
     5881 tttcaggaat ttttccattt tctctctatg aaattacaat gtacataata gttatatcta
     5941 tcttcttatt tttatgttgt actttatcta caatttttaa taagaaaaaa aaattaaaca
     6001 tatttttaaa aaattctatt ttaaatatat tatccattgt atcaatagtc tactttcttt
     6061 ttgtagtgct ttggggaata aactataata ggattccact agaaaccaca cttataaata
     6121 actataattt agaaaacaat acctctatcc aatcaaaaca acatagtgtt aaagagctta
     6181 ctaaattata taaatttctt ataattaaag caaatgaaac tagaaaactc acattacaag
     6241 ataaaaatgg tattgtaaaa tctaatacag attataatgg tgttataaat agagctcaac
     6301 taggatacga taatatcttg gatatattac ctagtgttag tggtagttat tctaagccta
     6361 agtatattat ttcatcaaat ctaatgtgct atacagggat aactggcata tactttcctt
     6421 ttacaggtga ggcaaatgta aatatagcaa tacctgactt gtatattcct tgtacagttg
     6481 aacatgaaat ggcacatcaa aggggatttg ctagtgaaga tgaggctaat tttatagcgt
     6541 atcttacttc tataaaacac cccaatattg attttaacta ttctggatat attttagctt
     6601 taaattatac tgcttctgct ttaagtaagg ttgattataa tgcatatgtt gatatttctg
     6661 ctggtatttc agattctgtc cgtagagatt taaaaaatga aagtaaattt tggcaaaaat
     6721 atgaaggtaa aattgatgag atttctaatg agtttaacaa ttcttactta aaagctaatg
     6781 gcgttactga aggcactcag agttatggta aaatggttga tttattgcta acttattatg
     6841 aattatatcc atataactaa acactacaat tcaaaacttt attcatatac agctggtatt
     6901 aaatcagctg ttttctattt aaataaatta tttagctcca agcaatctta tataatttga
     6961 aaatacttat actatctgta taacatttca attctaacta ttttagttaa aacatttcta
     7021 ttatcttttt taataaatat tttaactaaa atttttaaaa cataaaactt ccacattatt
     7081 tcactatcta tataaaaaga taaaaattta catataaaat tacataacta aaaaatcata
     7141 aatttataga aaacatatat tcaaaataaa aaagcatgta tcaaaacttg acacatgctt
     7201 ataatcttat gtatatatgc cattttgtca caataaaaat ttccttgtaa cactgacatt
     7261 tttaaacaaa atagttctta tttaattaaa ttaacattag ttaaattcgt acttatttta
     7321 ctatcattaa acattactta tagtaatttt ctaatacact atttgaaact tccttccctt
     7381 gaaaactttt caaccaattc tcatatgctg aatttttcca tacactacca tcattaaatg
     7441 ttacttgttt tacacaagat ataatgtact ttacaccatg ctctccagtt gattgtttcc
     7501 atttatcaaa caataaaaaa gcatcatttt gattcttacc tggtgctatt gcattttgct
     7561 ttgcatattc tttctccatt tgtttccatt ctttcaacca attttctacc catgccttgc
     7621 cttgttgttt ttcaaacata cttattatat cctgtggtgg tgattgttgc atttttctat
     7681 aagtatgtga atatgatttt ggagacacag gagatatgcc tgtaacaata ccataatcta
     7741 caccagctgg gctaaaccca acacttccta cttcgccatc tgctgccaca ttttgagcat
     7801 cccagtatag ctcaagtggt tttccatttt tatcatatgc taagcacgta atttcataat
     7861 ccataattgc tttttcactg ctatttgtta agcttataac attgctaggc caatgatatg
     7921 gagtatcatc atactgtatt ttgctattat ttataaataa tggaatattt tcattgtaaa
     7981 caagaccttt actactttga atatctgata ctttctttgt gatagtactt actgtacttg
     8041 ctaagtatac acttgttttt tctgaattgg tcgtactaag agaaaatgtt gttgtcgttc
     8101 cagtaatcag caaagcaaca actaaaatag gtaagctaga tattttttta tatctcacag
     8161 ttcttcaccc cttcttctat taacttataa taaaacaatt taccaaaaca ttaaaatcta
     8221 tgttaacata atttaccaga tgttctagta actatatcct atcatagtta ctatcaagtt
     8281 tcaatactat tgatggaaat tttcactata ttaaatcttt ttttaatttt ataaatttac
     8341 aatataattt aactcataca tatatagtca aattagagta taagtttctt acatttaatg
     8401 aagtttataa aataaaaaat gactagataa ttaaatactg taaataaaaa ctcattacaa
     8461 aagtatttaa aaatctagtc aattaatata tatctaaatt gtatactata atttctcaat
     8521 ctcttctagc aactcatcaa ctaatttgtt accatcaatc attctcacta tttcaccctt
     8581 tttaaataaa aggccttcac ctttacctcc agcaatacct atgtctgctt ctctggcttc
     8641 accaggtcca tttacagcac agcccataat tgcaacagtg atattttttt ccatactacc
     8701 aatcttttct tcaacctcat ttacaacact aattaagtct atattacatc taccacatgt
     8761 aggacatgat ataactttaa tcttatcatt taaaagtccc aaacttctta aaatttgttt
     8821 accaacaata acctcttcaa cagggtcacc tgttaaagat attctaacag tatctccaat
     8881 tcctttaaga agaagtgctc ctacccctat agaagacttt attgttcctt tgtgaacact
     8941 tccagattct gtaattccaa tatgaagagg ataatcaact ttttttgata ttaattcata
     9001 tgcatcaaca gttttataaa tatcggatga ttttagagat ataactatat tatgaaaatc
     9061 taagtcttca agaatcttaa tatgtcccat tgcactttca actaatgctt cagcagttgc
     9121 agagccatac ttttttaaaa gttctttctc taaagaacct ccattgactc caattctaat
     9181 ttttaaattt ctttccttgc atttctcaac taccatctta actctctcta tacttccgat
     9241 attaccagga tttattctta ttccatccac accttgttct atagcttcaa gggctagctt
     9301 ataatcgaaa tgtatatctg caataattgg tatatttaca ctagacttta tctttgctat
     9361 attcttagca gctttaatat caggaacagc aactctaact atatcacaac cagcttcctc
     9421 tagtctcttt atttgagcta ttgtagcatc tgcatctctt gtatcagtat ttgtcataga
     9481 ttgtatactt ataggatttt ctcctcctat ttttacagtt cctacactta cttctctcgt
     9541 ctttcttctc tcgtaactca tattccacct ctatatatta ttttatattg aatctcttta
     9601 aatattaaaa tattataacc tacatgtata ttataaatta aaaagctatc tagttaaata
     9661 agaataaatt tcatacataa aaattaattt attcttattt aaaactacat agcctatttt
     9721 cttaaaatct ctaaaaaact aaatcattga aataatctta gtatatcttt atatgtgata
     9781 aaaagcataa ataacattaa agctccaaaa ccaacaacat ttatcatgcc ttctttattt
     9841 gggtcaagtt ttttaccacc tctaacagct tctagtaata gcataagtat tctccatcca
     9901 tcaagcgctg gtattggtaa tagatttact atacctaaat ttaagcttat gacagctgct
     9961 aaatacacta catttatgat gcctgtacga gcagcatccg ataccatacc aataacacct
    10021 actggtcctg ctacagcatt gccaatccct cctggtacac gtcctgtaat catttgacct
    10081 agaaatgtta gcatctgttt tgtcatatct acagtagttt tacatgcatt tactattgac
    10141 ccccaaaagt ctttttccct ttgtggttga atccctattt catactttcc attgttattt
    10201 ttaggtgtta catttacaac tttatcactt ccatctctag ttatactaag ttttaattct
    10261 ccaccagaag aagtgtttat tatatttgca acttcatccc aagtcttaac agaattacca
    10321 ttaattttat ttatcttatc tcctacttga agccctacag cctgtgcagg agtatctggc
    10381 attatctttc ctaatttagt cgttggagaa cctatataca aaaatactgg tactaaaagt
    10441 attgctgcta atattatatt gaatattgga cctgctacaa tagtactaaa tctttgtaat
    10501 atagacttgt ttccaaaaga atttggactt atttgctctt catcttcacc ttccatactg
    10561 acatatccac caattggaag tgctcttata gaatactctg tatctttttt tacactatat
    10621 atcttaggcc ccatacctat agaaaactca tttactttga taccacttct ttttgcaaat
    10681 ataaaatgtc ctaactcatg tattaataca attatactaa atagtattaa tgcggctatt
    10741 atagtcataa attcaccttc ctattttttt atcagatttg caacataagc tctactccat
    10801 ctatctgttt ctaagatttc ttctaatgtt ggttcactta tgctactatg aacttctaaa
    10861 gtcttctcta tgtaatatgg tatgtcatag aaaccaattt tatcctctaa aaattcactt
    10921 actaaaactt catttgctga atttaaaaca gaagaataag ttcctcccat ttttaaacac
    10981 tcatatgcca attttaaaca tggaaaagtt tctaaatctg gttcttcaaa agttaaagta
    11041 ctaaattttg aaaaattcat tctttcaaag ctactttcca ttctatctgg atatgtaaga
    11101 gcatactgta ttggtagtct catatctgga catcctaatt gagcaattat tgagctatct
    11161 gcatactgca ccattgaatg aattatgctt tgtgggtgaa ctactacatc aatattttct
    11221 tgttctaccc caaacaacca tctagcttct atcacttcaa gtcctttatt cataagcgtt
    11281 gaagaatcaa tacttatttt tcttcccata ctccaatttg gatgcttcaa agcttcattt
    11341 ttagttatat ttacaagttc tccctttttc tttcctctaa atggaccacc agatgctgta
    11401 agtattatct tttctatatt ttttttattt tctccattta gacattgaaa tatagcacta
    11461 tgttcactat caacaggaag aatatttaca ttgtattttt ctgcttcttt cattacaagt
    11521 tcaccagcgg ttaccaacgt ttctttattc gctaatgcta tgtctattcc cttctttata
    11581 gcacaaagag ttgggactaa tcctatcata cctactacag cagttaaaag gacatccact
    11641 tcatcaagtg atgatattat ttttaatcct tccattcctg ctaagacttc tatttctata
    11701 tttttaggca acatttcttt taatttcaat gctttatttt cctcaaaaac tgttacatat
    11761 ttaggtttaa actctactat ttgttctagc aataattcaa tattactgtt agctgatatt
    11821 gcaactatct caaacttatc tctatgttct ctaaccacat ccaaagtttg tttacctata
    11881 gagcctgtag aacctaaaat tgatatcttt ttcatataat aatcctttct aataataaat
    11941 ataccctatc aaaacattat aacactacta aaacaattat ttttactatt catagaaatt
    12001 tcatatatta ttccacagat taaaattaat ctattatttt ttgtattttg tggaatatta
    12061 taaaatattc tagtatcatt aaatgccctt ttttatttcc acaacatctt ctattttaca
    12121 gtctagtgct aaacatatct tctccattgt atccatagaa acagatttat catttcctag
    12181 tctgcctaaa acagaaatac ttatacctga tatatttaca aattccattt tctttatttt
    12241 tctgtcaatt aacaatttcc ataatggagc gtatgaaatc atgttatcac ctcgcaaaat
    12301 atattctatc acattttatc tattttggaa aatattcatc tatttggtta aataatcacc
    12361 tgattaagtg tcaaataatt ttatccttct aatatgaaaa attaatacta tactaaatga
    12421 ttggtctata acacatatga tgcattatta ttaacatata aaaattttta ttggcgctaa
    12481 attaaagtaa tatactatta ttatatttgc agatatataa gaaagatttt attatatatt
    12541 taaagtactt gattataata agagacaagg gctatgatgg atactataga aagttagaac
    12601 tatactattt tagaatgaac aattaaaata attaaaatta gtttaatctt ttattcttga
    12661 taaaataata ttaaataaat gcaaaaagaa tcttattatt agaaa
//
LOCUS       DAMJVM010000102        12576 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.106, whole genome shotgun sequence.
ACCESSION   DAMJVM010000102 DAMJVM010000000
VERSION     DAMJVM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12576
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.106"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(189..713)
                     /locus_tag="PC523_002936"
     CDS             complement(189..713)
                     /locus_tag="PC523_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2476438.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVKTDAADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(778..1530)
                     /locus_tag="PC523_002937"
     CDS             complement(778..1530)
                     /locus_tag="PC523_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2476439.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPENIKKIYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1561..2859)
                     /locus_tag="PC523_002938"
     CDS             complement(1561..2859)
                     /locus_tag="PC523_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HDF2476440.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHTVESTKEILDKNRFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGLLNDTLEKDNFLIKQIASNLDVNMEDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISIGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2876..5140)
                     /locus_tag="PC523_002939"
     CDS             complement(2876..5140)
                     /locus_tag="PC523_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HDF2476441.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5394..6212)
                     /locus_tag="PC523_002940"
     CDS             complement(5394..6212)
                     /locus_tag="PC523_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2476442.1"
                     /translation="MFKINNLTYQYEKNANALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEIFFKEEKLKYDKKSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGIDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHKNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6205..6903)
                     /gene="cbiQ"
                     /locus_tag="PC523_002941"
     CDS             complement(6205..6903)
                     /gene="cbiQ"
                     /locus_tag="PC523_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HDF2476443.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLHSFEIFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6906..7190)
                     /locus_tag="PC523_002942"
     CDS             complement(6906..7190)
                     /locus_tag="PC523_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2476444.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7180..7932)
                     /locus_tag="PC523_002943"
     CDS             complement(7180..7932)
                     /locus_tag="PC523_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HDF2476445.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLVGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAMPVFLAAALGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8119..8311)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8443..10215)
                     /locus_tag="PC523_002944"
     CDS             complement(8443..10215)
                     /locus_tag="PC523_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HDF2476446.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKAIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYAFVLYSMDKHPALDSMTRRIVNDIRNIDTK
                     QDIMEVLKELDVIYLTYFEYILNEEESSLIDENNVKNVEVDFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKSSNLADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRSVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKTRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSLKNSENIFEYFCAGNNRDGLAIKSICSGLINRYEENKILIVLSDGRPNDIKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10229..11158)
                     /locus_tag="PC523_002945"
     CDS             complement(10229..11158)
                     /locus_tag="PC523_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDF2476447.1"
                     /translation="MNIIDNLRAQGVDEKLIEDVMYFRNYYGLEKDLEYRVTRSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIQFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            complement(11564..12172)
                     /locus_tag="PC523_002946"
     CDS             complement(11564..12172)
                     /locus_tag="PC523_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDF2476448.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVVSSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
     gene            complement(12349..12465)
                     /gene="rrf"
                     /locus_tag="PC523_002947"
     rRNA            complement(12349..12465)
                     /gene="rrf"
                     /locus_tag="PC523_002947"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         3982 a         2276 c         1312 g         5006 t
ORIGIN      
        1 gtaaaatttt tttaatagag gtttattttt tagttaaaat cctacaatat atagttttct
       61 ctaatacaaa aaaattaaaa agtgaaatgt atttgtctaa aaacataaca acatttcact
      121 ttattctcat tattaagtaa tatattttta atttttattt ataactcaat ctagttttta
      181 tataagtttt agtttttttc tacatctgaa taatctattc caaaagtttc tacagtaaca
      241 gatttcataa tttgtggagt ttgtggctta tcagctgcat ctgttttaac tgatgctatt
      301 tcatcaactg tatccatacc ttctatcact ctaccaaaac ctgcatattg accatctaag
      361 tgtggtgagt ttttatgcat tatgaaaaat tgagaaccag ctgaattagg tgccatagtt
      421 ctagccattg atatgactcc tctttcatgt tttaaattgt tagtaaatcc attaccacta
      481 aattcccctt ttattgagtg acctggaccg cccattcctg taccatttgg gcaccctcct
      541 tgtatcataa atcctggtat tactctatga aatattattc cattgtaata tccttcattt
      601 actaatgata caaagttttt aactgtattt ggagctatat ttggatataa ctcaatttta
      661 atttcttttc cattttccat ttctatagtt actataggat ttttattttc cataatttat
      721 taccttcctt ttcattagtt aaattacata attacattta ttatattata ctctaaatta
      781 aagtctatca agtatatttt ttcctatttc gtatccttcc tgataaatag ttttacaaaa
      841 ctcatcatca aagttaagtg tatctttagg agcaaattga gtgttaggtc taactactat
      901 tattttatct tcatcgtata tttttttaat attctctggc aactcataat tatattttgt
      961 ggatattaaa ataattttat ccacattatc cccaattaat ggttcaatta atgcactcct
     1021 aattaaacct ccatctaatt tgaaaccatc ataaattcct tcttgcacat ctttcacaaa
     1081 ttgttcttta aatccaagag ttgcatttgg atttgatggc aataacccac tgtatgaaat
     1141 actgataatt gcttcattac gaggcatttt tgatacatca acaatctttt ctgatactac
     1201 cttattatca acttcaacat aattaacata aaagttcatt tcttctatat tactatcatc
     1261 tagttccctt aggttgtcta tagcaggaga attatccaca gtattattta ctattttcac
     1321 attcccattc tggatatctt ttatatttgt ccacattttc tccaagttat caacattccc
     1381 tgtgtacaaa taatatccat ttattgctcc tattgatgtc cctgaaatag aataaaattt
     1441 atttattcct ccatcataca aggctttaac tactcctgct tgataagccc cttttgctcc
     1501 tccaccttct aagcataatc ctaatttcat agtctcatct ccaaattgtt tttcttatat
     1561 ttatatttca aaaaacttac taaatgtttt atatatacta tagtggtcct cgacatttcc
     1621 taattctctt atagagtgca ttgctaaaat tggacttccc acatccacag atggtatatc
     1681 tatatgagta gaagatattg gacctatagt acttccacct ctttcatctg acctattaac
     1741 aaattcttga tattcaacat tagcttctct acatatattt ttataaatag ctatagaata
     1801 tccatcacta gtatatgcct gactagcatt tatctttata actggacctt ttcccataat
     1861 tggtttatta gttgggtcat gcttttctgg aatgtttgga tggatagcat gagctaaatc
     1921 tgatgatatc ataaaagatg aataaataga ttcaaagaat ccttctctat ccttacctat
     1981 tgatatgcaa atcctctcca atatatttaa caacatgttt gaatctgcac cttgtttagt
     2041 agagctacca acttcttcat tatcaaatac agccattaca tttacaccat gttgaccttt
     2101 agtattaaca agagcattta agctagcatg taccattgca agattatctt gtctcccaat
     2161 agatataaat tcatcattag cacctattaa acttcctttt tcaaattcat ataaaaataa
     2221 atcaaaatct ataatatctt ccatatttac atctaagtta gatgctattt gctttataag
     2281 aaaattatct ttttctaatg tatcatttaa cagcccaact aaaggtaaca tatctttttg
     2341 cttattatat gaatgaccat catttatgct tctatttaaa tgtatcgcta gatttggtat
     2401 tatacaaatt ggctttttaa agtctataag cctttcattt ggttttaata tactatcacc
     2461 ttttaaaact actctaccag ctatcccaag aggtctatcc aaccatgtac ttaatattgc
     2521 tcctccatag cattctgtat ttaacttcaa ataagatttt tcagcttcta tttctgcatt
     2581 tggtttaata ctaaatgtag gagaatctga gtgagagcct attattctaa atccatctct
     2641 ttctatatct tctgaattta ctacaaaagc tactatagct gataaattct tagtaacata
     2701 atactttcca ccaaccctta gattccatct ctgattcaaa actaattctt caaatctatt
     2761 tttatctaag atttccttag tactctcgac tgtatgaaaa gacgacggac tatcatatat
     2821 gaagtctatt aagtttttag caaagtttat actatccatt cctcatttac ctccattata
     2881 aaatatttag tgttgtatat tctttattta tttttttaat tccctgttgg tcaaaagcta
     2941 tagtaacatc tgtcccaatt atagaaacta ctgtcccaac cccaaattta ggatgatgta
     3001 ctttagaacc aagttttata tcatctggat tagtttcttt attagaatct tttattgtag
     3061 cattaacttt atctgcaact ttagatttac ttatagtact catatacttt ttagtatatt
     3121 tatctaacat attataactt gctttagaat agcttagttc tttttttact ttgtacaatc
     3181 tttctataca ttcttctggc agttcttcca taaatctgga agctattgca acatttgttt
     3241 taccatataa tgttctcttc tccgtcaatg acatatatag ctcttcttta gctctagtta
     3301 taccaacata acaaagtctt ctctcttctt ctatttgtga atcactcata gattttatag
     3361 ctcttgatat aggaaataat ccttcttcca tacctgttaa aaacacaaca gggaactcta
     3421 aacctttgga tgtatgaatt gtcataagcg aaaccctatc attgtcttcc tcatcatttg
     3481 attcagatgt aagtgctata cttgttagga atgtctctaa gtctttattt tcataagtat
     3541 catcattact actttcaaac tccaaagcta tagatataaa ttctctcaaa ttatcaattc
     3601 tgtcttgagc ttcttcacct ttacctgtta aatctttttc atttttattt cttatctcta
     3661 caagctcgtc catatatcct gtagtgtcca aaactttttc tataagttta ctaacaggat
     3721 aaacttcttt tatagttcta agagtcccta taatatcaac aaatccactt atacttgctc
     3781 ttgccttggt agatatatct gaatttgttt ctatatcaat tagtactgag tatatgctct
     3841 cttgttttaa attagctcta tcttcaattt tttctatagt tctaagccct atacctcttc
     3901 taggaacatt tataatcctt tttatagata tatcatcttg tggattttga ataactctta
     3961 aatacgccaa caaatccttt atttcttttc tctcatagaa ttttgtacca ccatatatat
     4021 tgtatggaat ttgacttcta tttaaagcgt cctctacagg tcttgcctgt gcatttgctc
     4081 tataaagtac tgcaaaatcc ttataaggtc tattttcttt tctagctatt tgtgcaattg
     4141 aatcagcaat gaaatcagcc tcttctattt cgctaccagt taattgtatc tttataagtt
     4201 ctccttcttt tttttcactc caaagctttt ttctttttct ttctatatta ttagatataa
     4261 ctttattagc tgcatctaat atcacttgtg tagacctata attttgttct aattttacaa
     4321 catgtacatt gtcataatct ttctcaaatt caagtatgtt tcttatatct gcgcctctcc
     4381 atccataaat actttggtca tcatctccaa caacacaaat attttgatgt tctcttgcta
     4441 ataattttat caattcatat tgagctttac ttgtatcttg gtactcatct accattatgt
     4501 atctaaatct acttctataa tatgctaaca cttcatcatt agctttaaaa agttctacag
     4561 ttttaagtat taaatcatca aagtcaagtg cactatttct ttttaaacgg tcttgatata
     4621 acgcataaat atctgcaatc ttaaccattc tattatctgc catattgata tctttaaact
     4681 gctttggagt ataaagtttg tcttttgctc ctgaaattgc acttataact gcttttggtt
     4741 caaacacttt atcacttaaa tttaattctt ttagacagtc tttaaccaat gtaacttggt
     4801 ctgctgaatc ataaattaca aaacttctat tgtaaccaat cttatttata tcttttctta
     4861 gtatacgaac acagcaagaa tggaatgtac ttatccacat atccttagtt tcaggaccta
     4921 tgttttgttc aactctttct ctcatttcat ttgcagcttt gtttgtaaat gttatagcta
     4981 atatatttgg tgcttgtaca cctttatctt ctataagata tgctattctt gttgttaata
     5041 ccttagtttt acctgaacca gcaccagcca gtataagtac tggaccttca gtcttttcta
     5101 ctgcttctct ttgagcagga tttaatgtat ctaaattcat atttctcctc ctgttaacag
     5161 ccataaatat attttaatct aaaatatgaa ttacttcaaa ttataattat gtattatatc
     5221 aatagattat ttccaatata ttacaattaa ataacatttt tccatataat ttatttcatt
     5281 gtttaataaa aaaaataacc ttatgatttg gggaatcata aggcaccttt aaggggtcta
     5341 ggttaagggt tattatatat aaatctattt ttttaaaata ggtttagggc taattattta
     5401 tttgtattaa gttctctttc tttccaatac ttatacaaat cttcttcttt cttaaataaa
     5461 ggtaaattca tatttttatg tacttttact aaccatgggg attctagacc agcttgtata
     5521 atattatttt catttataaa tacattttct gcctttcctt catcaatcaa aatcccctta
     5581 tccaatacat atatgtaatc acatatttca tacattaaat tcatatcatg actggatatt
     5641 actattttta tattattttt atttaatccc tttattatat ctacgataga cctagtgctt
     5701 actgggtcta gaccagctgt aggttcatca agtaatacaa tttcattttc cattgctata
     5761 actgatgcta ttgccaccct ttttttctgg ccataactaa gaaaatgtac tggtctgtct
     5821 ataaaatcta ttccattaac agctactaga gctttgttta ttctctcttt aattgttttt
     5881 tcatctatac caatgtttct cattgcaaat gctatatcat catatacttt tgaataaaaa
     5941 atttgttttt ctgggtcttg aaacacaata cctacatttt ttcttagatt gtataaactt
     6001 tttttatcat actttaactt ctcttcttta aaaaaaattt ctccagaggt aggctttaat
     6061 atccccatta agttcataaa taatgttgat tttcctgaac catttgaacc tattatacct
     6121 ataacatttc cctttgaaaa atccatatta atgtttaata atgcattggc atttttttca
     6181 tattgatacg ttaaattatt tattttaaac atcattatcc cctactatgt gaaatttacc
     6241 atcatacaat tttatgtcta atgatataca catatcatca taccttgtca taagtctttt
     6301 gtacagcata cttccaagaa ttcctaagga tttatatgaa gtttttaaat ttatataacc
     6361 aaatcttagc tcttgtgact tgtatatttc tgaaaattct tctaaaaata tgaatataaa
     6421 cctatatatt aacattgata actcaacgaa agtgtcaggt aaatgaagtt ttttaaaaaa
     6481 gaatattaat tgattaaaag gtgttgtaag tatacaaaaa tacacacaag tcaaacaaga
     6541 catagctcta aataacacgt gaatagacat gtcaatagac gcctttgata ttcctacatt
     6601 aaaactaaaa atttcaaaac tatgtaataa atctgcttta ttaaaagata tatttacaag
     6661 agttattcct atactaagaa ataaaaagta cattggtatc ctaagtaatt ttaaatagtt
     6721 ttttaaatct attccagcca cacaaacaac cagaatactc attaaaatca taattccact
     6781 taaaataaat atattttgta ttaccataga agcaatcaga aagattactc ctatggctac
     6841 ttttttgttt ggatttactt tacttaatct atttgtatat gcatatttat caattataag
     6901 catgattaac ttctctttcc tttttgaaga cctaatatgt atcctattac accagcacct
     6961 aaagctgctt gtgcagagaa taataaactt tctatttctc cacttggtgg ctcatatgga
     7021 ctgctaaacc atggtttgta atttggattt atcttagtaa tttcactttc agcttgacca
     7081 tctgctcctc catattcagc acctgagttg acaagtaacg ggaatataat aagagccact
     7141 actaataaaa gtaataatat gttagttttt gtttttgttt tagcactcat tagctttaac
     7201 tcctttatca ttattttcta gtatgctttt ttctccattt tctgtaatta aattatatac
     7261 aattacagta agtataccct ctgctattgc tattggtatt tgagtcataa agaaaattcc
     7321 taaaaactta attgctgaag ccatcactcc acctgatgga tctggaaaag ctagtgctaa
     7381 ctgtattgaa gtaattgtat atgtagccaa atctcctagt gctgctgcaa gaaatactgg
     7441 cattgcatta tttccatctt ttttctttaa tgctttaaat attaaaaatg atacaattgg
     7501 tcctataata gccattgaaa aagcattagc tcctaaagta gttatgcctc catgtgctaa
     7561 tagtaatgct tggaatatta aaactatagt tccaagaaca aacattacgc ttggtccaaa
     7621 cattatagct ccaagaccta ctccagtagg atgtgagcaa cttcctgtta ctgaaggtat
     7681 ttttagtgct gacaatacaa acacaaatgc tccacatagc gctaataata ctttcttttt
     7741 agggtcttgc tttactattt ttccaattga ttttagtcct actaagaaaa atggtataaa
     7801 tataactccc catgctatag accattttac tggtaaataa ccttccatta tgtgcattgc
     7861 atttgatgta actggagtta aaatgattaa cataagtgca gctataaccc ctaatttgat
     7921 attttgtttc atttttagta tcccctctct ccctttctaa taatcaaaat ataagtgata
     7981 catgtctgat ttattttaaa taaaataaaa aaacctaact cgatggttag gcttctaatt
     8041 ttccacaatc caaatacaaa cacataaaac aaacatatct gtataattat tataagaaac
     8101 tttctccatc cccgaagaaa ttactttaat attttaggca ggtctcctga cttagcttct
     8161 tcctaatcct cttccttccc atgagatata aaaaatatca tcatagtggc taacaaaagt
     8221 ttcgtctgca tcacagtagc gggggctgta aaggaattta acctttttcc ctattaatct
     8281 tgcttgaaga acctataata ttatatattt aatttttaaa ttattcttaa attgtattct
     8341 atataaatca ttgtatacct tttctgtgaa aatttctaca ttttttataa attttattca
     8401 tttttatatt tttatgtata taaattcttt tttacaaact tattagtttc taattatctt
     8461 ctttaaatac attgaaacca catcagaaaa tctagttata tctcttgaat atataaaatc
     8521 atttccatat atcaatcttt cagcttcaag ttctgattct ttacctgtaa acactcctaa
     8581 tactaatata ttttgctttc tagcttttct cacttcattg gctgtatctc tcagtccaac
     8641 tatacctcta taagcttttt ctcctctcaa agtcctctca ctactttttc ctatctttat
     8701 atcatttggt cttccatcac taagaactat caagatttta ttttcttcat atctatttat
     8761 tagaccacta catatagatt ttattgccaa accatctcta ttgttaccag cacaaaaata
     8821 ttcaaatata ttttcactat tttttagact atcatcataa tctttaaatc tttttaatat
     8881 agtatagtct aaaaaactag aaaatcccat tactctattt ggtatgccaa cttctgttag
     8941 agcatttgca attatgtaag cttgaattgc cacatttcct tgatttctac tttgagagcc
     9001 acttgcatcc aataatatat caataacgta tttccctttt tcattttcta tatctttata
     9061 aaatatttta ttattattac ttctaccaac tctccaagct ctatttgcta atatagtacc
     9121 acaatcagaa tatactctag ttttagcatt ttcttctatt aaaattcttg acagactttc
     9181 ttttaatctc tttatttgct gtctatacat tctagcatgt tctctaaatt ttcctaaatt
     9241 attttctttt tgtcttgtta catattttag ttgaaataca ttattacact cacttctaag
     9301 aactccatct gtaaagtgta ttctacaacc ttcatgaaca cttctacaat tttgtgtctc
     9361 aattttttta atttcacctt tactcaaata agtttttcca taataatgtt ctatttttga
     9421 atatatctta tttgcagtgt cttcatcaac atatataact cggtctgcta aattgctaga
     9481 tttggaatca tcaaaatccc ctacaccttc tacaagcatg gatgaagata tcgaatctat
     9541 atcactttca ataatatttt cttcttcatc actataaagt tcttcataca tgaaatcagc
     9601 aaatgtatca aagtctacct ctacattttt tacattattt tcatcaataa gactactttc
     9661 ttcttcattt aatatgtatt caaaataagt taaatatata acatctaatt cttttaaaac
     9721 ttccataata tcttgtttgg tatctatatt tcttatatca ttaactattc ttctagtcat
     9781 actatctaat gctggatgct tgtccatact ataaagtaca aatgcatatt taattttatc
     9841 cataatatta tcattgtgta ttttggtaaa acgtgaaaca atatctttat aagcatttct
     9901 ccttatgtct tctattcctg gtctatcttt aatagccttt tcttcaacta ttgtatttaa
     9961 tattacttga attagaccca gtataatatc tttgttgtat ccttttctta ttctatttgt
    10021 aagatatttt ttaactatat cccaatcaac atactttctt ctagcacctg caattattgc
    10081 aaaatacaga cctatttcct ttgaagaata atctttttct gatatatcta tactctcatt
    10141 gtagtcgcca gctattgtcc aggataagtt attggctcta ttttcaaatt cataatcatc
    10201 atatatccat ttcataatct cacctatatt aaatatgact tattggaaac acatctatac
    10261 tctcccattt attaggtatt ctagttttaa ttacatctcc aaccatttct ttctcataca
    10321 catcaaaagt ctttcctgta agccccatat taattgcaag ggttggtttt aagcctcttc
    10381 ttatggtctt aagagatgcc ataagaccac gtaaatcaat agctttacta gatatctcgc
    10441 cattttgaga cttcaattgt aaatctaaaa atattcctgc aaattgtatt aatttttctt
    10501 catcagcatc tgaaaattca ttttttaaaa tcatcattaa cttatcttct tctataggag
    10561 gaatatctat taccataaat ctagatacca atgcctcatt aagttctttt gttcctgcat
    10621 attcataatt catagtccca ataaatcttg ttgcaggatg tagatttact ctctcatacc
    10681 caggtacatc aattatcctt ctatagtcta aagctgaatg taccacaact atagcatcat
    10741 tttttgccat attaatttca tcaaataccc caaaacctcc attaatagca cactcataaa
    10801 ctgaacctct tctaagttta acttcattgt ctataaacgt atctgtacct ataagtgttg
    10861 aactatctgt gtttatatga aatgatgtat tccactgagg tctattaaat atttccccta
    10921 aattatcagc aagtaggttt tttcctgttg cctttggtcc tgataataat atattttctt
    10981 cctctaaaat cgctgctata cacatagata atatgtcttt tccataaaaa taagttttag
    11041 accttgttac tctatattct aaatcttttt ctagaccata ataatttcta aaatacatta
    11101 catcctcaat aagtttttca tctacccctt gtgcccttag attatctatt atattcattc
    11161 ttcttacccc ttttatataa atagtatttg tttcatttct taacaccatt ttagcataaa
    11221 aggatataag ctagaattga tttcataaat taatgacaac tcttcctctc ttttaaaaaa
    11281 aactacctat atgtactttt tatgtattat ttttgttaaa tgtctgaaag ttatcagatt
    11341 actattcatt acaggattta taagttgagg cttgtgaatg tatttgatta ctatatattg
    11401 ttctagtttt gaatttatta tcttgacaat atttcatctg ttttacatca aaaatccccg
    11461 ctttcatata gaactagaaa cggggatttt gattattata aattttctca aacatatgaa
    11521 ctttagaata gctgtagaaa aagatacaaa actttttcat ttcttataat cttatcttgt
    11581 gatgtttttt atgtataaat gacttaataa caaatccaat aaaaattatg gatattggta
    11641 taattagaac taggaattta taatcattat aatgtgacat tattcttacc caactctcag
    11701 aaaaaatata ccctatactg attaatgaag tattccatat ggttattccc acgatagatg
    11761 atactacaaa agttataata ttttgacctg ctatacctgc tataaaagat atgtatgttc
    11821 tacataaagg gattaatcta catacacaaa cagaaataga agagtattta ttaaaataat
    11881 cttgagatgc aaatattcct ttctttgcct ttggacatat ctctattatt ttatttataa
    11941 tagatttacc tccccaattt cccactgtat aacaaaatat cgaaccaagt atacctgcaa
    12001 ttatacttaa aattaaaatt gtcaaaaatc caaaatgatt tcttgcagca attgctccac
    12061 ataaaggtaa aacaacttcg ctaggtaatg gaaaacaagc atactcaatc ataattaata
    12121 caaatacaga gattaatcca tattgtaaaa taaaactatt gattacagac acttacttat
    12181 ctatctcctt tcttatttaa aggttttaag ggtacatatt ctaaggattc tcaccctata
    12241 tttaaagaat caatctcttt aaactattat atgtatttac agaaagcatt tataacatac
    12301 tttaaaaata gtaccttaaa ataagaaact taataaataa aaaaaattac gtggctacgt
    12361 cctactctcc caggcagttt cccaccaagt accatcagcg ctagagagct taacttctgt
    12421 gttcggaatg ggaacaggtg tatcctctct gctattgtaa ccacataatc tttatgctta
    12481 atatttctat taagttttta ataagtttat agaatctagt aatactagtc cttttagagt
    12541 taacactcta aaaactacac atatatctta gtaagt
//
LOCUS       DAMJVM010000103        12457 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.107, whole genome shotgun sequence.
ACCESSION   DAMJVM010000103 DAMJVM010000000
VERSION     DAMJVM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12457
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.107"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            82..708
                     /locus_tag="PC523_002948"
     CDS             82..708
                     /locus_tag="PC523_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2476449.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMNNKLYSTVESNIIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKAIMHSRMNTIFASENTNDTPSNNGIKMLDKCLSTGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLTENFLDILVGGLYNENSQQI"
     gene            686..1090
                     /locus_tag="PC523_002949"
     CDS             686..1090
                     /locus_tag="PC523_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDF2476450.1"
                     /translation="MKIHNKSSNKKKKYLKISITIFFVFICIIILIVSNLKPENLVVK
                     DINIANIKNGIYTGSADNKLVKATVSVEVNNGKIQNINILKHDNLLGKPAEKITTNII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            1093..1566
                     /locus_tag="PC523_002950"
     CDS             1093..1566
                     /locus_tag="PC523_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HDF2476451.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNITIVDINNNNKIELSK
                     FDKIIIGSSIYAGSVSKKIRLLCNDNLELLNKKQVGIFLCCGFSEEADKYLKSNFPSS
                     LLESANAIGIFGGEARLEEMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLISLNN"
     gene            2116..2781
                     /locus_tag="PC523_002951"
     CDS             2116..2781
                     /locus_tag="PC523_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDF2476452.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGMATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKANIIMAVIALVVATIQFTLYDKKLNNS
                     HKK"
     gene            2803..3735
                     /locus_tag="PC523_002952"
     CDS             2803..3735
                     /locus_tag="PC523_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDF2476453.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVIFAKYEEPNENFGKEHADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFANSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            3756..4397
                     /gene="pcp"
                     /locus_tag="PC523_002953"
     CDS             3756..4397
                     /gene="pcp"
                     /locus_tag="PC523_002953"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDF2476454.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQKHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTVI
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIK
                     GGFIHIPYMTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(4517..4849)
                     /locus_tag="PC523_002954"
     CDS             complement(4517..4849)
                     /locus_tag="PC523_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2476455.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            5115..5735
                     /locus_tag="PC523_002955"
     CDS             5115..5735
                     /locus_tag="PC523_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2476456.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKEAI
                     PGCISCASCFETGKCILNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(6048..6872)
                     /locus_tag="PC523_002956"
     CDS             complement(6048..6872)
                     /locus_tag="PC523_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HDF2476457.1"
                     /translation="MDFLKKGISGFTIKVLALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMVVEGFCYTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGVIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIVLGIFTVTLLFKATNKII
                     ILFMILVPVPFLVEGGPVWIILGIIFYLCRGKKFSLSICYVLMCIFIFTAMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(6883..8286)
                     /locus_tag="PC523_002957"
     CDS             complement(6883..8286)
                     /locus_tag="PC523_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2476458.1"
                     /translation="MKWKITRNFIFTIVFVVISVVIINIISILYVISTDNFLKVVDSR
                     NNPEEFARSFEKDLYEKDGELKLSKIGADKLEKSNSWIQVLNDLGEEVFGVNVPKDTP
                     QKYTPFQMVNNYKYIETEYVNFVLEKYLNKKHLNIIVGIPSRDVSRIILTYSQNNTKK
                     TLNKIIIIILVIDGMVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FSEDEIREYAEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKVNLVGLVRNIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVKIVKKD
                     NIEIIIKDSGVGISKNDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYL
                     NSEIGIGTEIIIEINQN"
     gene            complement(8290..9015)
                     /locus_tag="PC523_002958"
     CDS             complement(8290..9015)
                     /locus_tag="PC523_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2476459.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLSNIYTAKTRKEGLEVFK
                     NINPDLIILDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDSQNENTDSDNNEEKTISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFANNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLSVKED"
     gene            complement(9127..9900)
                     /locus_tag="PC523_002959"
     CDS             complement(9127..9900)
                     /locus_tag="PC523_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2476460.1"
                     /translation="MMYLVKIEMIKILKSKSVWIIWLFLLFFGFVLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKNGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNEPIHNLWYFGNSSLNLTVIQMYIIVILTTIFAS
                     FVFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     IVRYNVPSILIGFSIVISIILMIVLTKITYKNFTSSKRF"
     gene            complement(9906..10793)
                     /locus_tag="PC523_002960"
     CDS             complement(9906..10793)
                     /locus_tag="PC523_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2476461.1"
                     /translation="MNKLEIKNLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQIFYNGEDIFKMDDDYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLKGKYLSSKIEELLDLVGLYDVRDKSIGKFSGGMKRRVGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKISRNKIIVLSTHIISDIESVAKETIMIKDGKLLMKGTHKE
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSIVLRIVSEDIPIGDNIQPVQ
                     AKFEDVYMFYFDLEDAREA"
     gene            complement(10806..11828)
                     /locus_tag="PC523_002961"
     CDS             complement(10806..11828)
                     /locus_tag="PC523_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476462.1"
                     /translation="MVNWEIKKLIKSKSMFISLGILILILSINIFVKPVLETENYYID
                     KERGRIEDTRNSEEIGNYKFQNKINVLKQLSSQKDMDVFSKKIKSISTNKLYNLIINE
                     YKDVSFWKVFNYRATNSLINVGMLIIIVIIISNIYVDELVFSVRDIILSSKEKKKALN
                     SKIVIAFLVPLTIYSVYLIGIFITTCMQQGMPINGDLEAFRIVDNIATLKCNSSIINY
                     VISNIGIEFLMFEGLAMIALLGSFISKSSITSISFFIFTIVITKIISVIHILPKNLLL
                     AISTVNYYDLIFGFNKIIGNYLGEIIIFKIHIDIVNLLIGVLVAIFIGAMSFCFIIIK
                     SDYISK"
BASE COUNT         4537 a         1766 c         1470 g         4684 t
ORIGIN      
        1 taatttattg acagtattaa acttaaatta tacaataaca gtgtaaatta aattaacact
       61 gttaagtgaa gaggtgatta aatgagtatt aaagaaagac gtaaaaatga aaaagaagaa
      121 atgaagaaaa agattatgga tgctagcatc gaaattataa atcaacatgg atatgaaaat
      181 ttatcaataa gaaaaattgc tacaaaaata gaatattctc caactacaat ttatctttac
      241 tacaaagaca aagcagagat tatcagcgat atgaacaata aactttatag cacagtagaa
      301 agtaatatta ttgacattat gaataattgt tcgtcgctac caatagataa acaaatcata
      361 gaaattatga ctagctttat aaaaactctt tctagtgaac cagagatggc aaaagctatc
      421 atgcatagta gaatgaatac tatatttgca tctgaaaata caaatgatac gcctagcaat
      481 aatggtataa aaatgcttga taaatgcctt tccactggga ttgaacaggg aatatttaaa
      541 aaaaatatag ataaaagctc atggatgctt ataagtgcat tgttaggatt cgttttatgt
      601 gttgttgaaa ataaattata ttctttacat aatttttctc aacttacaga aaatttctta
      661 gatattttag tgggaggact ttacaatgaa aattcacaac aaatctagta ataaaaagaa
      721 aaaatatctc aaaatatcta ttactatatt ctttgtattt atatgtatta ttattttaat
      781 tgtaagcaac ttaaaaccag aaaatttagt agttaaagat ataaatattg ctaatattaa
      841 aaatggcatt tatactggaa gtgctgataa taaactagta aaagctactg tgtctgttga
      901 agttaataat ggtaaaatcc aaaatattaa catcctaaaa cacgacaatc ttttaggtaa
      961 acctgctgaa aagattacaa caaatattat taaacaacag tctttagatg tggatgctat
     1021 aacttctgca acttatagca gtaatactat acgaaaagct gtagaaaatg ctttaagaaa
     1081 aggagaataa ttatgaatac tattattata tattcaagta aatatggttg tacaaaagac
     1141 tgtgcaaata tactaaaaaa taaactctca gataatatca ctattgttga tattaataat
     1201 aataacaaaa ttgaattatc aaaatttgat aaaattatta ttggaagctc tatatatgct
     1261 ggttcagttt caaaaaaaat acgattacta tgtaatgata atttagagtt attaaacaaa
     1321 aaacaagttg gtattttcct ttgctgtggc ttttctgaag aagcagataa atacttaaaa
     1381 tcaaattttc catcatcctt gttagaaagt gcaaatgcga ttggaatttt tggtggtgaa
     1441 gctcgattgg aagaaatgaa atttttagat aaacttatta tgaaagctgt ttcaaaaggt
     1501 aattatgaca gttttagaat atcacaggat aatatagata attttttaat aagtcttaat
     1561 aactaaaata aaactaatta tctctatgat aaactctttt atttctaagt aatattgctc
     1621 aaaatagtct ttagacttta ttaaaatttt atttttctta atgaaatcta ataaaactag
     1681 tactcaatat atgttttgtt aatcataata aaaaggtagt atctgatgaa ctttttagtt
     1741 cattggacac tacctttttg catggaatca aatatatttt tatcatttca acattaaaaa
     1801 atagacttct ctctattttg agatattctc tttttataat ataatattta atttaaaact
     1861 actattttaa atattaccac atacttaaat tataaacata tcaaaatcaa ttcatattag
     1921 attaaaatat aatcttactt attcaaaatt tctttacata acgcttattt actttgacga
     1981 tttaaaacat cttccctatt ttttattaaa ttttataata atagtcctat taacgcaaat
     2041 actataaaaa gagtttaact ttatccatta aataaattta aattaaaaca tatagaaatg
     2101 gaaggagaac aatttatgat aaaactaatc ggtatattga tagttgtaat tggatttata
     2161 cttaaaattg acacattatt tactgtatta ttagcaggaa tggcaacagg aattgttgca
     2221 ggattagatt ttaatcaaat ctttacaatc ttaggagatt cttttgtctc aaacagaggt
     2281 gtatcattat tcatattaac tttgccagta atcggtgtat tagaaagata tggtttaaaa
     2341 caaagagctg tttctttaat agaaaaatta aaaagactca caacaggaaa agtacttact
     2401 atatacatga tagctagaca aatagctggt gctctatcaa tcagaatgag tgggcatcct
     2461 caatttgtaa gaccacttgt taatcctatg gcacaagctg ctggtttaag taatagtgat
     2521 gaactaaaag aatcggatga agaagccata aaagcattgt ctgccgcgtc agaaaactat
     2581 ggaaactttt atggtcaaaa cctatttgca ggtagtagtg gagtgttact tatagcatct
     2641 actctcactc aatttggata caatgtaact ggtcttaata ttgtaaaagc taatattatt
     2701 atggcagtaa tagcacttgt agtagctacc atccagttta ccttatatga caaaaagtta
     2761 aataatagtc ataaaaaata gatttattta ggaggaataa aaatgcaaaa ttttataaat
     2821 atatcattag aaatttttta cgcactaatg ggatttttaa tgattgtcat agcatataag
     2881 tcttttacta ccataaacaa caataaaaaa tatggtacat cattattttg gatacttata
     2941 tcattgccat tcatatttgg tagattaatt ccagctaata taatcggaat aatattaata
     3001 ttatctagtt tattaacact ttctaaacaa gttatatttg caaagtatga agaaccaaat
     3061 gagaattttg gaaaagaaca tgcagataaa cttaaaaaca aaatatttat accatcatta
     3121 atattagcat ttgctgcagt agttgtggct atgagccttt ctaattttgc taattcttca
     3181 caatttgcaa taggtgtagg ttcaataatt gcattaatat cagcccttat aataactaag
     3241 gcaaaacctg ctacatcagt tcaagatggt tcaagattac ttcaacaaat gggtcctgct
     3301 agtatgttac cacaactttt agttgcattg ggagctttgt ttactcaggc tggagtcggc
     3361 gaagttattt caaccatgat atctggtgta gtccctgctg atagcagatt atttggtgtt
     3421 atagcttatg tattaggtat ggttatattt accatgataa tgggaaatgc atttgctgca
     3481 ttttcagtca taacggcagg aattggtatt ccttttgtat tgtctcaggg tggaaatcct
     3541 gctataattg gagcattagc attaacagca ggatattgtg gaactctact tactccaatg
     3601 gctgctaatt ttaatatagt tccagcagcc ttactagaat gtaaaaatga ttatattgtt
     3661 ataaaatatc aagctccagt tgctttggta ttaattatag cccacatatt agtaatgtat
     3721 tttttaggat tttaatataa ttaggaggta aaataatgaa aattttgtta acaggatttg
     3781 acccatttgg tggagaacca ataaacccgg ctcaagaagc agttgaaagg gtcaataata
     3841 atataaatgg tgcagaaatc ataaagataa ctataccaac agttatgaca aagtctgttg
     3901 aagctataga taaagctatt caaaagcaca atccagatat agtaatatcg gttggacaag
     3961 ctggaggtag atttgacatt acacctgaga gagttgctat aaatatagat gattttagaa
     4021 taaaagataa tgaaggtaac caggttatag atactgttat aaaagaggat ggtgaacctg
     4081 catatttttc aaaattacct gtaaaagcca tggtaaaaca tatgaatgaa aataaaattc
     4141 cagctagtgt ttcaaacact gcagggacat ttgtttgtaa tcatgtgatg tatggaatcc
     4201 tttatatgat agacaagaaa tatcctaata taaaaggtgg atttatacac atcccatata
     4261 tgacttcaca agtaatagat aaaaaaaata caccatttat gtctttagaa gaaatagtaa
     4321 aaggtttaga attggctata gaagcttgta ttatatacaa ggaagatgta aaggaaattg
     4381 gtggcgaaat ttcataataa attttttagt atttttataa attaaaaata ctaactatta
     4441 atttaaatat agactgtgag tagattattc aaacaatttt actcacagtc tttttatagt
     4501 atctacagat acaaaattaa ggtctattcc tctctcccca ctcacacatt gcatctaaaa
     4561 ttggaatcag agattttccc ctttctgata agctatattc taccttagga ggtatttgag
     4621 gatattcagt acgaataatc aagttatctg cttctaattc ttttaatgat aaactcaaag
     4681 ttttatagga gatagtattt atataacgtt ttaatgcatt atatcttaca acttcaaatt
     4741 ctacaagaca gtaaagaatt atcattttat atttaccact aatcagtgaa agtgtataac
     4801 taaatcctgt ttcacttaaa tctgtatttg atatacagtc tatactcata tattacactc
     4861 tccttttggt tagtattgaa caaaaatgtg cgtacttgtt tttagaataa tttataataa
     4921 aattatagtg taagaataat tgaaagtcaa ttttataata ggaggtgtat ttactaaaaa
     4981 aggagttatt ttaaatagga ttctaagatt agatatttct atataaattg ataatattca
     5041 tgaaaatcct attttataaa aaataatatc tcttaaaaaa tctattaaat aaaattcatt
     5101 ggaggaaatt tataatgaaa gttttattga taaacggtag cccaaaccaa tatggatgta
     5161 cttatacagc actgaatgag attactaaag tattgagtaa acacgatatt aaaacagaaa
     5221 tactgtattt aggtaaagaa gcaatcccag gatgtattag ttgcgcatcc tgttttgaaa
     5281 ctggaaaatg tattctaaat gataaagtta atgaattgat tgaagatctc gataatattg
     5341 atggtattat tattggttct cctgtttact tttctagtgc aactggtcaa ttaacttcat
     5401 ttcttgaccg tctatttttc atagctggaa gtcgtatggc tacaaaatta ggtgcttcag
     5461 ttgtgtcatg tagacgtggt ggtgcaagtg caacatttga ccaactaaat aaatattttt
     5521 caatcagcaa tatgcctata gtatcatcac agtattggaa tcaggtacat ggctttactc
     5581 cagaggatgt tatgaaagat gaagaaggat tacaaactat gcgaactcta ggagaaaata
     5641 tggcatggct tcttaaatgt atatcagcag gtaaaaaagc gggaattaaa gaaccacaat
     5701 atgaagaaac aattatgact aattttataa aataaataaa aaaatgaagt aatattaatt
     5761 aaaacactaa tctataaata agcataaata taggataaaa agcaataaaa cagaggtatg
     5821 aattttatat taaacctctg ttttattatt ctattaataa tcaaacccta tatcctatca
     5881 tatatttcta gatagattat aatataatat tctaatgtta ttggaattac aataactgaa
     5941 tttctgttca taattttaaa tattctacaa atctcatcta aataacaatt ttaattgacc
     6001 taaaacaatc ctcatattat tcattttgta ttaaatatca cttacaatca aaagccattt
     6061 attaagtata ttcctaagat atacaatata tatacatgga ttggatagta taaataaaat
     6121 aaatacttca tactcttccc tttttctcca ttgtaaagca acattaaagg tagagaagca
     6181 atcatcatcc attggtaatt ttgtaaaata gaatttttta aactataatc tccatttgac
     6241 attgctgtaa atataaatat acacattaaa acataacaaa tacttaaact gaacttttta
     6301 cctctacaca aataaaaaat aatcccaagt ataatccaaa ctggcccacc ttctaccaaa
     6361 aatggtactg gtaccaatat cataaataga ataataatct tattagtggc tttaaaaagt
     6421 aaagttactg taaatattcc taaaactatg ggaataatta tcatcaataa tcctataata
     6481 aaatatttaa tttctttctc ttttctaaat ttctttataa actctattgc ttgtaaataa
     6541 attgctatta aaaataatgt tgaaaaaatg ttatttataa ttataactcc ttctggatgt
     6601 gaaaatacat tattaattat ttgatttcct atagccataa taacagacca aatatacaat
     6661 ctacttatat atttttttct gttactagtg taacaaaatc cttcaacaac cataaatata
     6721 aatagtggtg ctgatattct gcctacccaa tgaaaccaaa ttggtatttg cattgtttgt
     6781 ggcataaatg ctgctatatg gtcaaaagtc ataaatatta atgctagtac cttaatagta
     6841 aacccactta ttcctttttt taaaaaatcc atatcatatc tcctaatttt gatttatctc
     6901 tataataatt tctgtaccta ttccaatttc actatttaaa taaattttac ctttatgaat
     6961 ctctattatt tctttagaaa tagccatacc aagacctgaa cctttatgca tctcacctgt
     7021 attagtaccc ctatagtatt ttttaaaaat atgtttcaaa tcatttttgc ttattccaac
     7081 tccactatct tttattataa tttctatatt atcttttttt acaattttta cacttatcaa
     7141 agttccttcg ctattatgaa ctattgaatt aaatataaga ttggttatag ctcttctaaa
     7201 taagattgaa tctacatata cttcaattaa atcttcattg ctttcaaact caatattcct
     7261 atttttatat ctattgtcat ttaaaatatc aattattata tttctgacta aaccgactaa
     7321 atttactttt ttcttgtcca aaactatagt attatttttt aatcgagtag ataaatttaa
     7381 atcatcaact aaatctttta tatacttaga cttattatat ataatttctg catactctcg
     7441 tatttcatct tctgaaaact cataatcttt gtcattaatt atctctgcat acccttgtat
     7501 agatgctaga ggtgtcttta tatcatgtgt tatatttgcc agccattctt ctctcaactc
     7561 ttcgttttct tttctctcaa cttcatttac tcttaaagta tcagctaaca tgtttatatt
     7621 tttaaaaact tcttcatata tacctctgtc cttaagatat aaactataat ttccattagc
     7681 catagtctca atactccata gtacacttga tattggtttt gtaagttttc tactaaataa
     7741 atatcctaca cctaaagcta ccatgccatc aattactagt attataatga taattttatt
     7801 taaagttttt ttagtgttat tttgagagta ggttaaaatt attcttgata catctctact
     7861 aggtatccct actattatgt ttagatgttt tttgtttaaa tatttttcta ggacaaaatt
     7921 aacatactct gtttctatat acttgtaatt atttaccatt tgaaatggtg tatatttttg
     7981 gggagtatcc tttggcacat ttactccaaa aacctcttct cctaaatcat ttaaaacctg
     8041 tatccaggaa tttgattttt ctaatttgtc agctccaatt ttggatagtt taagttctcc
     8101 atctttttca tataaatctt tttcaaaaga acgtgcaaat tcttcaggat tatttctaga
     8161 atctacaact tttaaaaaat tatcagttga aataacatac aatatagata taatatttat
     8221 aattactact gatatgacta caaacacaat tgtaaatata aaatttctag taatcttcca
     8281 cttcatattc taatcttcct ttactgacaa tttatatcca agacctttta ttgtcagaat
     8341 atattcaggt tttgaaggat tgtcctctaa tttttcccta atttttctta tatgaaccat
     8401 tatagtattg tcaaacccaa cataatcttc tccccacact ttatcccata gtttttcttt
     8461 acttataatc tgattttgat tatttgcaaa taaagatagg atttttaatt cctttgcaga
     8521 caatcctata gactttccat cttttataag ttctgcccta ctttcattta atatatacgg
     8581 gccaaatgat atagtttttt cttcattatt atctgaatct gtattttcat tttgagaatc
     8641 actaaaataa cttaatcttc tcaaatgagc tttaacccta aaagctactt ccttaggact
     8701 aaaaggtttt gtgacataat catctcctcc aattgctagt ccaagtaatt tatctaattc
     8761 ttcagtttta gctgataaaa atatgattgg agaattggag gtttttctta tttctttaca
     8821 aatatcataa ccttcaccat ctggtaacat aatgtctaat ataatcaaat caggattaat
     8881 atttttaaat acttctaatc cttcttttct agtttttgca gtatatatat tactaagacc
     8941 ctcttttttt aggacagttt ctaaaagctt taatatttct acttcatcat ctattattaa
     9001 aacacggttt tccataaata acctcctata ataatttacc tatatattag catatatttt
     9061 atctaaaatt catatatctt attttaaatt aaactaagat ataaataaaa ttactattat
     9121 ctttagttaa aatcttttgc ttgatgtaaa atttttatat gttattttgg ttaacactat
     9181 cattaatata attgagatta ctatagaaaa acctatcaaa atactaggta cattatatct
     9241 aacaatttgt tgactcatca taatccaatt tggagtcact gacataaatt ttatagcttt
     9301 atctggtata aaacctacac taaagtatgg aattgccatt atcagtccac ctagtataaa
     9361 tgggattaca gcagatttaa atatagatga aagtgttaaa cctatttgtg caaatacaaa
     9421 agaagcaaaa atagtagtca atataacaat tatatacatt tgtattactg taagatttaa
     9481 actactattt ccaaagtacc ataagttatg aatcggttca tttaaatttt taaaatcaaa
     9541 aaaactaaag cctctaattc ctactaacaa tataaaagaa atattaataa tggataaaat
     9601 taaaccatta gcaactaatt ttgataatac tactgatttt ttcccatttt tagttgtatt
     9661 tattaaacct gtcatattgg aatcatattc tttaatgtaa tttccagaag ttattataaa
     9721 cattatcaaa cctattagag gtattgagcc ttctatcttg taaaatatat ctgcataagt
     9781 atctaaaaca tcaaactttc taattagaac aaatccaaaa aatagtaaaa acaaccaaat
     9841 tatccaaaca ctctttgatt ttaatatttt tatcatttca attttaacta aatacattat
     9901 ttctactacg cctcccttgc atcttccaag tcaaaataaa acatatatac atcctcgaat
     9961 tttgcttgaa ctggttgtat attatctcca attggaatat cctctgaaac aattctaagt
    10021 actattgaat caatatcact ttgaatgctt acaaccttat atttagattg tatatcatca
    10081 acttcacatt catttttaac ttgaacatta tatactttat tttccatgtg gcttaatatt
    10141 tctttgtgtg ttcctttcat tagaagtttg ccatctttaa tcattattgt ttcctttgca
    10201 actgattcta tatcggaaat tatatgagta gaaagtacta ttattttatt tctagatatt
    10261 tttgctatta aatttctaaa tctagctctt tcctgtgggt caagacctgc tgttggctca
    10321 tcaaggacta ttatcttagg gtcgtttaat aaaacttgtg ctataccaac tcttctcttc
    10381 atacctcccg aaaattttcc tatggactta tctctcacat cataaagtcc tactaaatct
    10441 aatagttctt ctatcttact acttaaatat tttcccttta aaccttttaa agctccaata
    10501 tactgtaaaa attcttttgc tgtaaaattt ttataaactc caaattcttg tggcaaatat
    10561 cccaaaatgt ttctataatc atcatccatt ttaaatatat cttcaccatt ataaaatatc
    10621 tgaccttcat caggttttat caatgttgtt atttgtttca ttaaagtaga ttttcctgct
    10681 ccatttggtc ctaaaagacc ataaactcca ttttccagag ttactgtaat cttgtcgttt
    10741 gctacttttt taccatatat ttttgaaaga ttttttattt ctaatttatt catcttaact
    10801 ctcctttatt tacttatata atccgacttt attattataa aacaaaatga catagcacct
    10861 ataaatatag ccaccaaaac tcctattaat agattaacaa tatctatgtg tattttaaat
    10921 attataatct ctcctaaata atttcctatt attttattaa atccgaatat caaatcataa
    10981 taatttacag tagaaatagc taaaagtaaa tttttaggta atatgtgaat tactgatatt
    11041 atcttagtta taactatagt aaatataaaa aaacttattg atgttattga agatttactt
    11101 ataaatgaac ctaatagggc tatcatagcc aatccttcaa acattaaaaa ctcgatacct
    11161 atatttgata ttacataatt tattatgctc gaattgcatt tcaaagtagc tatgttatct
    11221 actattctaa aagcttctaa atctccatta attggcatac cttgttgcat acatgttgta
    11281 atgaatattc caattaaata aacagaataa attgttaatg gtactaagaa agcaataact
    11341 atttttgagt ttaatgcctt cttcttttct tttgatgata gaatgatatc tcttactgaa
    11401 aaaaccagtt catctacata aatatttgat ataattataa caatgataat taacattcct
    11461 acatttataa gtgaatttgt agctctatag ttgaacacct tccaaaaact aacatcttta
    11521 tattcattaa ttattaaatt ataaagtttg ttagttgata ttgatttaat ttttttagaa
    11581 aatacatcca tatctttttg acttgaaagt tgttttaaaa cattaatctt attttgaaac
    11641 ttataattac ctatctcttc actatttctc gtatcttcta tacgccctct ttctttatct
    11701 atataataat tttcagtttc taaaacaggt ttgacaaata tgttgattga tagtattaat
    11761 attaatattc ctagagatat aaacatactt ttagatttaa tcaatttttt tatttcccaa
    11821 tttaccaata tactaagcct cctaaatcat tttgactaca cttatatatt aaaacttaaa
    11881 tcttatatca atataatctt aaccttaaat aaagcttaaa ttatttactt tattgaaact
    11941 catcaaaaat atgcttttaa atttaatttt ttttaaaata gtctaaaata tatttatttt
    12001 ttattgtaat cttttagttt atctttttta taagatacct gctatacttt ttctaataag
    12061 attaagttta gtagttatat gagaaataca gatatttata taaagtttag aataaacctt
    12121 ttaacatcta taggcatgtt tatcattaca gttgtattta ttatttttgg tatttttatt
    12181 gagtgaaaat tttatctttg tctaaactgt aatcattgtt tagatttaag taatgagttt
    12241 ctaaagaatg gttcttattg tcctcattat ggaaagatta ttaagtagta attcttttgc
    12301 acattttatt tagtgttaat taataaaatt tgctctaggc ttatttctat acttcttgtt
    12361 aagttataaa agttttaaac atgttaaaaa gatataaaaa attattctag acaattctct
    12421 atatcttttg gtgtttataa atactttata tagcttt
//
LOCUS       DAMJVM010000104        12422 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.108, whole genome shotgun sequence.
ACCESSION   DAMJVM010000104 DAMJVM010000000
VERSION     DAMJVM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12422
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.108"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     repeat_region   66..2932
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=198..226
                     /rpt_unit_seq="ctttacataccacatagttaatataaaac"
     gene            complement(3211..3363)
                     /locus_tag="PC523_002962"
     CDS             complement(3211..3363)
                     /locus_tag="PC523_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476463.1"
                     /translation="MDNFLQGILASLVASLIVYLTSKLFKKVKSHSGRSDFSFELEIK
                     FKKNKH"
     gene            3883..4125
                     /locus_tag="PC523_002963"
     CDS             3883..4125
                     /locus_tag="PC523_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDF2476464.1"
                     /translation="MRNRQGIIKNQTFFEDVKLIDITPNLLKIYVNKIYKKYSLNSAK
                     NSIGFLNPLLHEAYILREIQEDVSKFVITSSKKIHL"
     gene            4191..4736
                     /locus_tag="PC523_002964"
     CDS             4191..4736
                     /locus_tag="PC523_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2476465.1"
                     /translation="MPIYLMLTLGLHFGEAVSLRWCDVELDEGIINIKQTMIYVREKV
                     TFKSPKTEKSKRILTTPIELINLLKEEKIRQNKLKLQDILKNELDLICLNKYFRPWKQ
                     QMFFRLFNRLLEKNNLRYIKLHELRHTNATLMLLSGTNIKTISERLCHADIKITMNRY
                     SLVLEDIDREASDNLSKILFK"
     gene            5281..6126
                     /locus_tag="PC523_002965"
     CDS             5281..6126
                     /locus_tag="PC523_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HDF2476466.1"
                     /translation="MNITKRMTLLLTLLIVILITGCSPKESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISLENLEDFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSTLYYAQACNYMNFDGFPDYPDATYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQDLNKSLYFNKDNEDPKSNLNTI
                     KKHLK"
     gene            complement(6350..6514)
                     /locus_tag="PC523_002966"
     CDS             complement(6350..6514)
                     /locus_tag="PC523_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476467.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
     gene            complement(7046..7377)
                     /locus_tag="PC523_002967"
                     /pseudo
     CDS             complement(7046..7377)
                     /locus_tag="PC523_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            complement(7637..8278)
                     /locus_tag="PC523_002968"
     CDS             complement(7637..8278)
                     /locus_tag="PC523_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDF2476468.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVILQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKQNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            complement(8259..8552)
                     /locus_tag="PC523_002969"
     CDS             complement(8259..8552)
                     /locus_tag="PC523_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2476469.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            8946..9005
                     /locus_tag="PC523_002970"
     CDS             8946..9005
                     /locus_tag="PC523_002970"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476470.1"
                     /translation="MVEYIYYYNNKRIKKIKLE"
     gene            complement(9521..10273)
                     /locus_tag="PC523_002971"
     CDS             complement(9521..10273)
                     /locus_tag="PC523_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDF2476471.1"
                     /translation="MFAIIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKVNNDLLKKLVPFTIVGSILGVRCVLSISEDILNVLV
                     VVMILIVAIYTFTSKNLGQEDNFESINNKNLRLGMLMAFIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYIGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(10334..11989)
                     /locus_tag="PC523_002972"
     CDS             complement(10334..11989)
                     /locus_tag="PC523_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HDF2476472.1"
                     /translation="MMDFSESNDEVQKFDWGEVTWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQIIDGKKISIKEKSIIYCPPYSEHEIINTGKVDLVI
                     LITYVPHKFSSLRQLPIVFSENTIQELINVNIIENLANQISNILKLRISVYDSKHKEI
                     FETKKETKFCDICKSIKECNRKLAKGMNNDNFEKMYHCRYGISSLEIPIVLEENIVGY
                     IECGNFIIYKSIDIDNNLSKLSSSSDVDIKYISKVYNELSLIPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKIRKENIRLEEALKKANSKLLEEDYIINPIDMFN
                     NNLKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLIYNDVRDIIKEMIFVLSRTALRD
                     LEDLKLISYLINKYNKKISLVDSDEDLWGVLFEFSNECIDKNRAFWRKDKGNLIENIN
                     KYIQKYYKENISLNSISEVFFISPNYLSSIFNERNKVSITEYINLLRVEESKKYLLDR
                     SFSISDICKKVGFNNSSYFSQIFKKFNNVTPNEYRKNILDNEH"
     gene            complement(12001..12363)
                     /locus_tag="PC523_002973"
     CDS             complement(12001..12363)
                     /locus_tag="PC523_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013281026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDF2476473.1"
                     /translation="MYESRENYLETILILEKKNGIVKSIDIAKQLNYSKASVSRAIGI
                     LKEYGFIIMEVDGDIVLTEEGRKKAEEIYEKHILITKFLVNTLGVSFDIAEKDACRIE
                     HVISKETFEGIKKYLDKN"
BASE COUNT         4763 a         1875 c         1201 g         4583 t
ORIGIN      
        1 aaatttaagt ttgtaataaa aaaagttaca ataaatagaa acattcttgt actcattgta
       61 acttcttaaa ttcaccactt agttaatata aagcgtaatt ttggaaaaaa taattacaga
      121 ggtaatacaa cctttacata ccacatagtt aatataaaat gaggcgtttt tttctgttcc
      181 gactcctgtt cctgtatctt tacataccac atagttaata taaaacaagg ctatacagga
      241 tgataattta aaagaaaact tcactttaca taccacatag ttaatataaa acagttcagt
      301 tggaaatata attgtagaac aatactctac tttacatacc acatagttaa tataaaactc
      361 attgatgcta acttaagtat tccttgtttt tgtgacttta cataccacat agttaatata
      421 aaacaaataa cttagtatgt ctaaatacaa attttagacc tctttacata ccacatagtt
      481 aatataaaac ttaatgatga aggaaaagaa tatgtagtat atgaatatct ttacatacca
      541 catagttaat ataaaacaag ttctatatac aactgtgcaa gttctgaatt aatctttaca
      601 taccacatag ttaatataaa acttaaccct cttgataaaa caaggaggta ttagatgaaa
      661 ctttacatac cacatagtta atataaaacg ataccgaagg taatttactt gcaatatcta
      721 aataccttta cataccacat agttaatata aaacgcggta aaatgttaga taaaactgtt
      781 acatttacaa actttacata ccacatagtt aatataaaac taagtttgaa cacacttatt
      841 ttcgacccct caatacactt tacataccac atagttaata taaaactttt tgagtatgga
      901 gataaagtaa aaattatact tccctttaca taccacatag ttaatataaa acttagcgtt
      961 gataaagatt ctatagtagt gagaggtgct ttacatacca catagttaat ataaaactaa
     1021 aaattgggat ataattcatc aacaaattta acatacttta cataccacat agttaatata
     1081 aaacattatc cttcatgtgc ttagctcttt tatatttttt ctttacatac cacatagtta
     1141 atataaaact ctagctatcc agaaacttat aaacctgctc cagattcctt tacataccac
     1201 atagttaata taaaacggat atgttgaagg aacacatatg ttgcaacaaa ccactttaca
     1261 taccacatag ttaatataaa actagcagga gatgatagag gggtatatca ccaggtgatc
     1321 tttacatacc acatagttaa tataaaacgt ttttctttat tagataaaag ttctccgtcg
     1381 aatctcttta cataccacat agttaatata aaacaatcat tccttttgtt tcatctttaa
     1441 gttttacgac ctttacatac cacatagtta atataaaact tagtaagcat aataattgct
     1501 tgtgtgatga tttgtttctt tacataccac atagttaata taaaacaaaa agaaactact
     1561 ttgtatcttt atgttggaat agactttaca taccacatag ttaatataaa actacttcgt
     1621 cttttttgat ttgaacgtct tattttcttc tttacatacc acatagttaa tataaaacga
     1681 gattttcata tagccataga ctgttttagt ttttctttac ataccacata gttaatataa
     1741 aactgcttgt gctgtaggtg ttccttgtgc tgtcatatct ttacatacca catagttaat
     1801 ataaaacttt aaataatacc ttagtagaat tatcagagaa aataacttta cataccacat
     1861 agttaatata aaactcctaa accttctatc ttctaaactc tcattacttt tctttacata
     1921 ccacatagtt aatataaaac atgataataa tgtagtgaaa cgaatagcaa aagagttctt
     1981 tacataccac atagttaata taaaacgagg cagaaataat taaatccaca atagatttag
     2041 ctatacttta cataccacat agttaatata aaacaaatcc ctgcaactgt agttggttgg
     2101 ggttataatg actttacata ccacatagtt aatataaaac taatgaaggt agcgttagta
     2161 ccaggacata cactaacctt tacataccac atagttaata taaaaccagt agctaattta
     2221 cttaatctat aagcatcaac ctcctttaca taccacatag ttaatataaa actatttaaa
     2281 tctgatgaat atactgctct tatttgccgc tttacatacc acatagttaa tataaaactg
     2341 tttgaacatg actttgaaca tgtttgtctt tcttctttac ataccacata gttaatataa
     2401 aacactaatt taaaaaacca cccacttgta gataatgcag ctttacatac cacatagtta
     2461 atataaaacc aaagtatagg tcagcatata aacctaaagt ggaagacttt acataccaca
     2521 tagttaatat aaaacgagag taaagattgt gatatagcat acaaaaattg gtctttacat
     2581 accacatagt taatataaaa cttaattgat tgttgagtaa atcctaattc ttttatttct
     2641 ttacatacca catagttaat ataaaactag tttattgtgt gtaaattctg cttcaaaact
     2701 tatttcttta cataccacat agttaatata aaactccact ccaagaatta ggaatagcct
     2761 tgctttcact ctttacatac cacatagtta atataaaact ctatgaccta ttggtgctaa
     2821 tccaagacct tctccacttt acataccaca tagttaatat aaaacttctt taggaatcat
     2881 cagtatggat gaatatcagt ctactttaca taccacatag ttaatataaa accccaaaat
     2941 aaacttagca tttccaacac ttacacatat gcaacactct aaaatttgca gtgagcgagc
     3001 agtggtgcaa atgataacac ttatcataca tcttcaacgc cttatatttc aatgcttaag
     3061 ccctatttta ttcaaaaaat cgaacactgc aaaatttcta tatctttatt atatcataaa
     3121 aatattattt ttgaatatct gcaccaattt gtggtataat aaaaacaaga agaactacaa
     3181 tctatttaga cgtagagtga agttcaaata ctaatgttta ttctttttga acttgatttc
     3241 tagttcaaaa ctaaagtcac tcctgccaga gtggcttttt acttttttaa ataacttact
     3301 agttaagtaa actattaaac tggcaactaa acttgctaat ataccttgta aaaaattatc
     3361 catacatatt cacctccctt ctatacgttg ggaggattat cttttgtatg aactccactc
     3421 tataaattgt agattacatc ttcttgctga aaagattata acatatattt ttacatattt
     3481 tccctactac attacttttt caaaaattca tctatatatt tatatacaac taccctatca
     3541 tcaaatagta tttataactt agatttgata taataaaacc ctatttattt aatgtatcaa
     3601 gaatacttat aaatgaagtg tacatatgaa tatcaaatca gcttttataa gaaaaaaagg
     3661 tgggaaattt catgtatatg tggaatatgt ggaagaagaa accgacaaaa agaaacaaaa
     3721 aagttatgga agttattaaa agaaaaagga tgctgaaaaa catttaattg aaataaaatc
     3781 aactatagac aacaataagt ttgttgctcc aagtgatgta acacttgtag aaaggtgcta
     3841 tagatacata gaggaaaaaa agataatatt tctctgtata ctttgagaaa tagacaaggt
     3901 ataattaaaa atcaaacctt ttttgaagat gtaaagttaa ttgatattac tccaaatctt
     3961 ctaaaaatat atgttaataa aatttataaa aaatattctt taaattctgc aaaaaattcg
     4021 attggtttct taaatccttt attacatgaa gcttacatac ttagagaaat acaagaagat
     4081 gtttctaagt ttgtaataac ttcaagcaaa aaaatacatc tgtaactaac ttttatacta
     4141 aagaaaaagc acagtttcta ttagaaaaat gtcttgaaac taacttagct atccctattt
     4201 atttaatgtt aacgcttgga ttacattttg gagaagctgt atcgcttagg tggtgtgatg
     4261 ttgaacttga tgaaggaatt ataaatataa aacaaacaat gatttatgtt cgtgaaaaag
     4321 taacttttaa gtcacctaaa acagagaaat ctaaaagaat attaacaaca ccaattgaat
     4381 taataaattt attaaaagaa gaaaaaataa gacaaaataa actaaaatta caagatatac
     4441 taaaaaatga attagattta atatgcttaa ataaatattt tcgtccttgg aaacaacaaa
     4501 tgttctttag actttttaac agattattag aaaaaaacaa tcttagatat ataaaattac
     4561 atgagcttag acatacaaat gcaactttaa tgttgctttc tggaactaat ataaaaacta
     4621 tttcagaaag attatgtcat gcagatataa aaataactat gaatagatac tctcttgttt
     4681 tagaagatat agacagagaa gcatctgata atttaagtaa aatactattt aaataatgct
     4741 aagttctatg tcagtaagat gtcagttaat cacatatttt tttaattgtc agttaaatat
     4801 caatcaaata tttattacta gatagtagca aaaactagta tttaactgct atctagtagt
     4861 attatataat agctaaacat aacataccta ttaatattac tgcttttagc aattttttta
     4921 ataaacatca atatgtaaaa attatcaata aatttttaca tcctcaatta atataaaaaa
     4981 ttactaaaga atatattctt tttaaaaata aaatatgaga acattattca ggtttcttag
     5041 atgcatctga agatgcataa agagtatgta taattggact attattgaag aagttaaatt
     5101 agacaaattt aatagtagca atttaaaata acaagaaaat aaacttataa actagttatc
     5161 taatttgttt ttttaatttt attaaatact gttctttatt tatactttaa agaagatgac
     5221 ttattcaata tttgatgcta gaatataatt aataaaaatt taattatagg tgattgtatt
     5281 atgaatatta ctaaaagaat gactttactc ttaacattat taattgtaat attaataaca
     5341 ggatgttccc caaaagaaag tgctagtgaa gttgcaaaaa aatacattga taagatgaat
     5401 tctggtgact atgaaacagc atatagttta ttaagtaaag atagcaaaaa aaaaattagt
     5461 ctagaaaatc ttgaagattt tcaaaatatt ttagttagta caaaaagaat aattagttat
     5521 aaaataggta aagaaaaaat cataaaaaaa tataagcatg atggaaaaga gtataaaaat
     5581 gttgttaaat tagaagaaac ttttaatata aaaaaccttc tttataaaaa agatgattct
     5641 ctaaaagtaa atagatattt tgtggttgaa aataataaat ataagctatt attgtatgaa
     5701 agtttcaaaa aagaatttgg cattaatagt atggaccttg ctcaacttaa gcttaatgat
     5761 tttggactaa aatactttaa agaagttgac ctcatactaa aaaaagctat tgctattaat
     5821 cctactgatt caactcttta ttatgcacaa gcatgtaatt atatgaattt tgatggattt
     5881 cctgattatc cagatgcaac atacaatgct ttagattcaa ttaataagtc tttaaagtat
     5941 ttagataaaa atgattctga ctacaaaaaa aatgcaagta atatatataa cttaaaagga
     6001 gttatactta tgtttcacaa aagatttgat gaagcacaac aagatttaaa taaatcctta
     6061 tactttaata aagataatga agacccaaaa tctaatctaa atacaataaa aaaacatcta
     6121 aaatagatat ttaatttttg aacctataaa atattttttt atatataatt cagtactgaa
     6181 tcataatctc tactaaacta attacaagtt taaattataa agacttattt ctatataaat
     6241 gttactgtat aacaataagt tttttattta aattttaaaa tattgcctaa aaatcataaa
     6301 aaagaaggta taaagagcta ccctcaagct caatatacct tataaatatc taaacttcat
     6361 tttttactaa tttttctata tctttgataa tatgttcttt agattttttt ccagctatgg
     6421 catcaaaaat tgcaacagcc tttttctctc caaacttttc tgctacatca ttaaagtcta
     6481 aaaatctaac actttctttt ggtttaccaa acatataaca cctctcatac atttttatat
     6541 atctattata acttctatag atttattttt caccaataaa cgttagacaa attagtccct
     6601 ttattttttt agactatctt taccttcaaa attcctaaca aaaccagttt attctactct
     6661 ataaataaaa atagaatatt taatttgtag aaaactgata atagaataca acaagtaaaa
     6721 ctttgtttaa tctatctgca aaagtcatct gttaaaactg ctatgaatta taaagagtat
     6781 tctagttcat aaaaatacaa atatgcaaat ataattacct taatattcaa tttatttaaa
     6841 ataaaaaata tacaaaagat ttaataataa caatatacaa ttctcaatac aatctatgat
     6901 gtaaataatt caagactact atatcatttt aaaatttata tatgcgattc taaaaactta
     6961 ataagaaatg aacttagaaa ataaaactta atttcaaata cttaaataga ctattattat
     7021 cctaaataaa ctacttctat caaaattata tattaaattc ttctaaaatt tttattgcaa
     7081 atcttgcgtt tggatatgta atagacccac caccaattac acctattttt atagcttcta
     7141 taatttcatc tttacttgct ccagaattaa cacaaccctc aatatgataa caaatatact
     7201 catcacatcg acttaaaatt gaaatagcta gcctcattaa ttccttattt ttttacttat
     7261 agccccttct gaataagtct tatcatcaag gcttccaaat actctaaaaa actcatttgt
     7321 ttcgcttaat tttttattgt aaaatcccct atattctttc atttctctag tattcatctt
     7381 ctacctacta tgtttttctt ataagaaaat cacactgtgc ttatattttc aacactaaat
     7441 acaaattttc aatttatgtt cattagccta acttatatta ttttttgaaa tctcaacaag
     7501 ttatcatata agtaagattt tttcagaact aaaatagaaa tcttagtaat ttctttatta
     7561 tctattctag ttctgaaata gattattttt atccaaatat tattatagtc attaattata
     7621 ttagttattt tttatattag ttatttttac ctgaaatttt atacatcaaa taagaatata
     7681 taaatggtag tattacaatt acaaagatta caaagatatt ataaaaagga atattaataa
     7741 atgcattaat aattaataat attccaccaa taagccaaat ccatccagct aaatggtgtg
     7801 tcttattcca attatcttca tcattaagag tccatggcaa aattattcct actgagtaat
     7861 tttgtttaca tttaggaaga tagtttccaa ataaaataac tattatccct aaaattaatc
     7921 ccgtataaaa attagattcc aaactaactc ctgttgaata taaaaccatc attatttgta
     7981 gaattactga aagtatagca actccccatt ttgataaatt ttgtataaca tttgaaaaat
     8041 tagctttctt aggctcattc tcaagtctaa accatgtata caaatggata catagcatta
     8101 acaatggtac accaaaagct gctataacct ttggagcata attatctgga tttccataat
     8161 tatcaaaatg aattgcaact tcatttggta atctattata aaaatatatt gatacaataa
     8221 atggtataaa acatattaaa gtagataaaa taaattgttt atttatcgct tttttcatta
     8281 tctttttctc ccttctccat aaattgtgaa aaccaaagca taacctcttc gaaaactgaa
     8341 atatttattt cgtagaaaat aaagttctta tactttgttt caaatatgag tcctgctttt
     8401 tttagttgag atagatgata agaaattgta gcattcgtca tattaaattt ttcactaatt
     8461 tctcctgctg acattttatt ctccctcaac ataaccaata tttctcttct tatagggtct
     8521 gaaagagctt tgaatgtttc tggaaacccc aacaccatca cctcactatt tagaatttca
     8581 tctaaataga atataacata acatccattt tatttcaata actatttaga aaaatttcta
     8641 aatagttatt tctaaatact tattgaaaat tttcatatta agattctcaa tttacttaag
     8701 tcgcttttat ttaattctta ttttatctaa ttttttaatt taacaaaagt gtactgcatc
     8761 acaattgtta aatcaaaatt ctaacaacag gtatacttct atatattatg tatatttttg
     8821 atttcaaatt aaaattagtt gaacctaatt taaaaggtag ttaaattcta tttaaataga
     8881 gaagaatgaa gcaaaaaaca tactgtagaa aaatacataa aaacttctat tagttagata
     8941 caactatagt agaatatatt tactattata ataacaaaag aattaaaaaa attaaattag
     9001 aatagccttg ttaaacgtat atttgaatat gaatataaaa ttgcataaaa ttaaaaatac
     9061 aggaatgaaa attccattcc tgtataaaat ctaacttttt agtctcagta caatcctatt
     9121 ttaagataag ctcttttgtt ttatattttc ttatatttaa aatataacgc atattttatg
     9181 caacatattc atatttttct ttatattctt tcatagcatc attccaagcg tcttgtactg
     9241 ttacacctct tttattagca attttttgtg ccagttcaat tatcttcata gctcttcttg
     9301 atatgttcat aaacattcct cctacaaatt accatatatt tcatacacat aaatcaagat
     9361 tatattttta tgatatataa tccttctatt gtaatattac aatttatatg tgtattatat
     9421 atgaactata ttagaagata attagaattt ttataaaact atatgattag gttaaatcta
     9481 aaagatttgc agttacttat aaatttaaaa gttctataaa ctaaactaat gtttgatata
     9541 ccaattttac gaccataaac aatgccatta ctaaaaatat tggctttatc atctttgaac
     9601 ctttctttat agcaacttta gcacctatat aagctcccat tatcatcact aatgcaaaca
     9661 ctatagcaat cttataatcg acttgaccat ttatcatgaa taataataat gaagttatat
     9721 tacttgttaa gtttaatatt tttgtatttg cagaagcatg taaaaaatca tatccatata
     9781 tttttataaa tccaaaagtc aaaaaagttc ctgtacctgg accaaaaaaa ccatcataaa
     9841 aacccattat aaaagccatg agcataccta atcttaaatt tttattatta atagattcaa
     9901 aattatcttc ttgtcctaaa tttttagaag taaaggtgta aatagctact attaaaatca
     9961 taactacaac caaaacattt aatatatctt cacttattga taatacacat ctcacaccta
    10021 aaatactacc aacaatagta aatggtacta atttttttaa aaggtcatta ttgacttttc
    10081 cagactttgc atatctataa gcgctactta tacatcctgc tgacgctgca aatttatttg
    10141 taccaatagc aagatgtact ggtactccaa ttgccataag aacaggcata cttataagtc
    10201 ctccacctcc tgctatagaa tctacaaaag ctgctaaaaa ccctcctata cataaaaaaa
    10261 taattgcaaa cataaaaatc ctccatcatt ctctttcaat ttttcttaca atatatatgt
    10321 tatatacttt tatttaatgt tcattatcta gtatattctt tctatattca tttggagtta
    10381 cattattaaa cttcttaaat atctgtgaaa agtaactact attattaaaa cctactttct
    10441 tacaaatatc actaatacta aaacttctat ctaacaaata ttttttagat tcttctaccc
    10501 taagaagatt tatatactca gttattgaga ccttatttct ttcattaaaa atagaactca
    10561 aatagtttgg acttataaaa aagacctcag atatactatt taaacttata ttttctttat
    10621 aatatttttg tatatatttg tttatatttt caatcagatt tcccttatct tttctccaaa
    10681 atgctctatt cttatcaata cactcattag aaaattcaaa taatacaccc cataaatctt
    10741 catcactgtc tacaagtgat atttttttat tatatttatt tattaaataa gatataagtt
    10801 ttaaatcttc taaatctcta agtgctgttc tggataaaac aaatatcatt tccttaataa
    10861 tatctctaac atcattatat attaatttat ttgtatctat tagattataa actccttcta
    10921 aattcatatt tttaatttct ttttcaatat aaagttctaa ttcaaaggga tattctttta
    10981 aattattatt gaacatatct attggattaa taatgtaatc ctcttctaat aatttactat
    11041 ttgctttttt aagtgcttct tcaagtctta tattttcttt tctaatcttc aatatttcct
    11101 catcttttat actaagttga ttttcaaaaa aattcctctt agctatttcc tgaataaatt
    11161 cagacatcat taataaattc tcttttaaca catataatct actctttgga attaatgaaa
    11221 gttcgttata aactttactt atgtatttta tgtcaacatc tgaactgctt gataatttag
    11281 ataaattatt atctatgtca atagacttgt atattatgaa atttccacat tctatatatc
    11341 caactatatt ttcttctaat actatgggta tctccaaact agaaatccca tacctacaat
    11401 gatacatttt ttcaaaattg tcattattca tacctttagc aagttttcta ttacactctt
    11461 ttatactttt acaaatatca caaaatttag tctctttttt agtctcaaat atttctttat
    11521 gttttgagtc atatacagat attctaagct tcaatatatt agatatttga tttgcaagat
    11581 tttcaattat atttacatta attaactctt gaatagtatt ttctgaaaat actataggta
    11641 actgtcttaa acttgaaaat ttatgaggaa cataagtgat taatattact aaatcaactt
    11701 taccagtatt tataatctca tgttccgaat atggagggca ataaattatg gatttttcct
    11761 ttatactaat ctttttgcca tcaatgattt gtattccttc tccctgaatc acatataaaa
    11821 gctgttcttc acttaaatga aaatgttttt cttgacagtt gcctggaaag aaccttacaa
    11881 ttccagcact aagtctatta aattgtgttt ttgatggttc atgtatccat gtaacctctc
    11941 cccaatcaaa tttttgtact tcatcatttg actcactaaa atccatcata aattcacctc
    12001 ttagttttta tctaaatatt tttttattcc ttcaaatgtc tctttactta taacatgttc
    12061 tattctacat gcatcttttt cagctatatc aaatgaaaca cccaatgtat ttactaaaaa
    12121 tttagtgatt aaaatatgtt tttcataaat ttcttctgct ttttttcttc cttcttcggt
    12181 taaaacaatg tcaccatcta cttccattat aataaaacca tattctttta aaattcctat
    12241 tgctctactt acacttgcct tactataatt taactgtttg gctatatcaa ttgattttac
    12301 aattccattt ttcttttcta aaataagtat tgtctccaaa taattttctc ttgattcata
    12361 cattttaaca tcctccatat ataacaactc tattatattc ttttaataat aattattaga
    12421 aa
//
LOCUS       DAMJVM010000105        12158 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.109, whole genome shotgun sequence.
ACCESSION   DAMJVM010000105 DAMJVM010000000
VERSION     DAMJVM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12158
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.109"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            162..950
                     /locus_tag="PC523_002974"
     CDS             162..950
                     /locus_tag="PC523_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2476474.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEDKIDILVNNFGSTNPSLDRDL
                     LTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPAFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            1423..5139
                     /locus_tag="PC523_002975"
     CDS             1423..5139
                     /locus_tag="PC523_002975"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDF2476475.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIMKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            5181..8666
                     /gene="rpoC"
                     /locus_tag="PC523_002976"
     CDS             5181..8666
                     /gene="rpoC"
                     /locus_tag="PC523_002976"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDF2476476.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNEKIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKSLGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREVDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            8956..9378
                     /gene="rpsL"
                     /locus_tag="PC523_002977"
     CDS             8956..9378
                     /gene="rpsL"
                     /locus_tag="PC523_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDF2476477.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            9501..9971
                     /gene="rpsG"
                     /locus_tag="PC523_002978"
     CDS             9501..9971
                     /gene="rpsG"
                     /locus_tag="PC523_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDF2476478.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            10020..12086
                     /gene="fusA"
                     /locus_tag="PC523_002979"
     CDS             10020..12086
                     /gene="fusA"
                     /locus_tag="PC523_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDF2476479.1"
                     /translation="MARKFPLEKTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFINKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         4712 a         1551 c         2488 g         3407 t
ORIGIN      
        1 aaatgtaaaa aattacatat tattttaaaa aaatacacga tagttttata aataaaatgt
       61 aataaataat taattaacaa atatgaattt taaaataagt taaacattta gaaaaatcga
      121 aatttgcagg atttaaagac atatttaagg aggagttaat aatggaaaaa ttacaaggaa
      181 aaattgcagt agttactgcg gcaacaaaag gtattggatt agcatcagca gagatattag
      241 caaaaaatgg agcaactgtg tatttagcag ctcgttcaga agaattagct aatgaagtta
      301 taaataaaat aagtgcagaa ggtggctgtg ctaaatttgt ctactttaat gcacgtgaag
      361 aagaaacttt tacctcaatg atagaagagg tagttaaaaa agaagacaag atagatatac
      421 ttgtaaataa ctttggttca acaaaccctt ctcttgatag agaccttttg actggtgata
      481 cagataattt ttttgataca gtaaatacta atttaaagag tgtatattta ccatgtaaag
      541 cagcaattcc tcacatgata aagaacggga agggaagcat agtaaatata tccagtatag
      601 gctcagtttt gcctgattta tctagaatag cttactgtgt atcaaaagca gcaatcaatt
      661 cattaactca aaatatagct acacaatatg ccaaagataa tgttagatgt aatgctgtac
      721 ttccaggtct tattgcaact aaggctgcat tagataatat gtctccagca ttcataaaag
      781 aatttttaaa acatgttcct ttaaatcgta taggagaacc agatgatata gcaaaagcag
      841 ttttattcta tgctagtgat gattcatcat ttataacagg agatttgctt gaggttgcag
      901 gtggatttgg tttacctact ccacaatttg cagataatat attaggataa ttaaattact
      961 aatatgtaat gaaggcttta tgtattagtt atagtatata aggatatttt tataaagacc
     1021 atagatataa tttatattgg aaaagtttgc ggttcaaaat tccaagttaa atttatttat
     1081 ggtttttatt ttgcatattc taataaattg tatatatttg tataaatgta attatttaca
     1141 atcattttaa tatagaatta taattaaata ggggggatgg tatttgtata aaattttata
     1201 tagaaaatta gtcctataaa tgaaaaatat ggattttata ttgggaaatt gtacataata
     1261 cataatatat gcttaaaata atttacaaaa aatcaaaaat aatgcatagt ttacatataa
     1321 gataaaaaat atattgaaaa atacttgaca ttatgctaaa attattagat gcgttagaat
     1381 gaactattgt tttattttta attattgaga ggtgaaaagt gaatgccaca tcctgtcacg
     1441 ataggtaaaa gaactagaat gagcttttct aaaattaagg aaatagctga tgtgccaaat
     1501 cttatagaaa tccaagtaga ttcctatgag tggtttttaa aagaaggttt aaaagaagtg
     1561 tttgatgata tttcaccaat agaagattat actggtaatc ttatattgga atttgtcgac
     1621 tattctctag atgataaacc aaagtacgat atagaagagt gtaaagagag agatgctaca
     1681 tattgtgcac ctctaaaggt taaggtaaga cttataaata aagaaacagg tgagataaaa
     1741 gaacaagaag tatttatggg tgacttccct ttaatgacag aaagaggaac tttcgttata
     1801 aatggagcag aaagagttat agtaagtcaa ttagttagat ctcctggtgt ttactatgcg
     1861 gaagaaagag ataaaacagg taagagattg atttcatcta ctgtaattcc taatagagga
     1921 gcatggttag aatatgaaac agattctaat gatgtaatat ctgtaagagt tgatagaaca
     1981 agaaaacaac cagttacagt tttacttaga gctttaggaa taggaacgga tgcagagata
     2041 attgaccttt taggagaaga tgagagatta tctgctacat tagaaaaaga taatactaag
     2101 acagtagaag aaggtcttgt agagatatac aaaaaattaa gaccaggtga acctcctact
     2161 gtagaaagtg catcatcttt actaaatgct ttattctttg acccaaaaag atatgaccta
     2221 gcaaaagttg gtagatataa attcaataag aaacttgctt tatgttatag aataatgaat
     2281 aaaatttcag cagaagacat aataaatcca gaaactggtg aagtatttgt aaaagctgga
     2341 gaaaaaataa gctatgattt ggctaaagcc atacaaaatg caggtataaa tgtagtaaat
     2401 ctactaatgg atgatgataa gaaagttaga gttataggaa ataattttgt ggatattaag
     2461 tctcacattg attttgacat agatgattta aacataaaag aaaaagtaca ttatccaact
     2521 ttaaaagaaa tattagatgg atatagtgat gaagaagaaa taaaagaggc tataaaatca
     2581 agaattaaag aacttatacc aaaacatata ttattagacg atataatagc ttcaataagt
     2641 tatgagttca atatattcta caatatagga aatattgatg atatagatca cctaggaaac
     2701 agaagaataa gatctgtagg tgaattgtta caaaatcaag ttagaattgg tctttcaaga
     2761 atggaaagag ttataaaaga aagaatgaca gttcaagata tggaagctat aacgcctcaa
     2821 gcgttagtta acataagacc agtttcagct gcaataaaag aattttttgg tagttctcag
     2881 ttatctcagt tcatggatca aacaaatcct ctatctgagt taacacataa gagaagacta
     2941 tcagcccttg gacctggggg tctttcaaga gaaagagctg gatttgaagt gcgtgacgtt
     3001 caccattcac actatggtag aatgtgtcca atagagactc cagaaggacc aaatatcggt
     3061 cttataaact ctctaggaac ttatgcaaag ataaatgaat ttggatttat agaatcacca
     3121 tatagaaaat ttgataaaga aacatcaaca gttactgatg aaatacatta tttaactgct
     3181 gacgaagaag atttattcgt aagagctcag gcaaatgagc cattaacaga agatggtaaa
     3241 ttcgtaaacc atagagttgt ttgtagaact gtaaatggtg ctgttgaaat ggttcctgaa
     3301 agtagagtag attacatgga tatatctcct aagcaagttg tatcagttgc cactgctatg
     3361 atacctttct tagaaaatga tgatgctaac cgtgccctta tgggagcaaa catgcaacgt
     3421 caggcagtac ctctagttag aagagaagca ccaattatag gaactggtat agaatacaga
     3481 gcagcaaaag attctggagc agttgttgtt gctagaaact ctggtatagc agaaagagta
     3541 acagcagatg aaataattat aaaaagagaa gatggaaata gagatagata taatctactt
     3601 aagtttaaac gttcaaactc aggtacttgt ataaatcaaa cacctataat aaataagggt
     3661 gaccagataa tgaagggtga tgttatagca gatggtccag caacagactt aggagaagtt
     3721 gcacttggaa gaaactgcct tatagcattc atgacttggg aaggttataa ctacgaggat
     3781 gctatattaa taaatgaaag attagttaaa gaagacagat tatcaacaat tcatatagaa
     3841 gaatatgaat gtgaagctag agatacaaaa ctaggaccag aggaaataac tagagatata
     3901 cctaatgttg gagatagtgc aattaagaac ttagatgaca gaggtataat aagaataggt
     3961 gcagaagtag attcaggtga tatactagtt ggtaaagtaa ctccaaaagg agagactgaa
     4021 cttactgctg aggaaagatt acttcgtgca atatttggag aaaaggctag agaagttaga
     4081 gatacttcac ttaaagtacc tcatggtgaa tctggtataa tagtcgatgt aaaagtgttt
     4141 actagagaaa atggagatga tttatctcca ggagtaaatg aattagtaag atgttatata
     4201 gctaaaaaga gaaaaataaa agttggagat aaaatggctg gtcgtcatgg taataaaggg
     4261 gttatctcaa gagtgttacc tgaagaagat atgccattca tggaaaatgg aacaccacta
     4321 gatataatac ttaacccaca aggtatacca tcacgtatga acattggtca ggttctagaa
     4381 gtccatttag gacttgctgc taaaacatta ggatggtatg tagcaacatc agtattcgat
     4441 ggtgctaatg agtatgatat aatggatgct cttgaagaag ctgggtatcc tagagatggt
     4501 aaattaactt tatatgatgg tagaacaggt gagtcttttg acaatagaat aactgttgga
     4561 tatatgtatt acttgaaact acatcacttg gtagatgaaa aactacatgc aagaagtact
     4621 ggaccatact cattagttac tcaacagcca ctaggcggta aagcacaatt tggtggacaa
     4681 agatttggag agatggaggt ttgggctcta gaagcatatg gggctgctca catacttcaa
     4741 gaaatactta ctgttaagtc tgatgacgtt gtaggtcgtg ttagaacata tgaagctata
     4801 gttaaaggtg aaaatatacc tgaaccagga atcccagaat catttaaggt tcttataaaa
     4861 gaacttcaaa gtttatgctt agatgtaaaa gtattgacag atgaagacca agaaatagaa
     4921 gtaagagaat ctgtggatga agatgataca ataggtgaat ttgagctaga tgttgtaaat
     4981 catatgggag aagttgaaga aagtaatata atagaagaaa ttgaagatga ttttgcagaa
     5041 aacgcagaag atgaggatat agagaattta gaagaattca ctgaagatga tttatttgaa
     5101 gaagaaatag attttgatag tgatgacttt gatatgtaaa tttaattaat gtaaatctca
     5161 aatagaaggg agagaactcc ttgtttgaat taaacaattt cgagtcgata aaaatagcat
     5221 tggcttctcc agaaaaaata agacaatggt ctaggggaga agttaaaaag ccagaaacta
     5281 taaattaccg tactttaaaa ccagaaaaag atggtctttt ctgtgaaaga atatttggac
     5341 cacaaaaaga ctgggagtgc cactgtggta aatatagaag agttagatat aaaggtgtag
     5401 tttgtgatag atgtggagta gaagtaacta aatcaaaagt aagaagagag agaatgggac
     5461 atatagagct agccgctcct atgtctcaca tctggtactt caaaggtata ccaagtagaa
     5521 tgggactttt acttgacatg tcaccaagat cattggaaaa aatattatac tttgcatcat
     5581 atgtagtagt tgatccagga gaaactggat taaacgaaaa acaattgctt acagaaaaag
     5641 aatacagaac tgctcttgaa aagtatggat atacttttac tgtaggaatg ggtgcagaag
     5701 ctgtaaagac gttactacaa aatatagatt tagaacaaca aagtaaagac ctaagagcgg
     5761 agttaaaaga tagtacaggg caaaagaaag ttagaacaat aagaagatta gaagttgtag
     5821 aagcatttaa aaagtctgga aataaaccag aatggatgat tttagatgcg ataccagtaa
     5881 taccaccaga tttaagacca atggtacaac ttgatggtgg aagatttgca acttcagacc
     5941 taaatgattt atatagaaga gttataaata gaaacaatag acttaaaaga ttattagagc
     6001 ttggagctcc agatataatt gtaagaaatg aaaaaagaat gcttcaagaa gctgttgatg
     6061 cactaataga taatggtaga agaggtagac ctgtaacagg acctggaaat agaccactta
     6121 aatctttatc agatatgtta aaaggtaaac aaggtcgttt ccgtcaaaac ttacttggta
     6181 agcgtgttga ctattcagga cgttctgtta tagttgttgg accagagctt aagttctatc
     6241 aatgtggact tccaaagaag atggcattag agctattcaa gccatttgtt atggataaat
     6301 tagttaaaga aggatatgca cataatataa aaagtgccaa atctatagta gaaaaagtta
     6361 agccagaagt ttgggatgtt ttagaagatg ttataaaaag ccatccagtt cttcttaacc
     6421 gtgcgccgac tctgcataga ttaggtatac aagcatttga acctatctta gttgaaggta
     6481 aagctataaa gctacatcct cttgtatgta cagcttacaa cgcagacttt gatggtgacc
     6541 aaatggcagt ccatgtacct ttatcagtag aagcgcaagc agaagcaaga ttcttaatgc
     6601 tttctgtaaa taatatactt gctcctaaag atggttcacc tataactact ccatctcagg
     6661 atatggtttt aggctgttat tatctaacaa tagaagcaca agacggagct aagggaacag
     6721 gtatggtatt taaagacttt aatgaactat tacttgctta ttacaataag tcagttcatc
     6781 tacatgcatt agtaaaactg aaggtaacac ttgaagatgg aagaagttca ttagttgaaa
     6841 gtactgttgg aagatttata tttaatgaaa aaatacctca agacttaggt tttgtagata
     6901 gaaaagaaaa cccatttgct cttgaagttg atttcttagc agataaaaag tctcttggta
     6961 aaataataga taaatgcttt agaaaacatg gtaatacaga gacagctgaa ttgctagatt
     7021 atataaaatc tttaggattt aaatactcta cattaggtgg tataacagtt gcagtagatg
     7081 atatgagtgt tccagaagaa aagaaagtat ttatagctga ggcagaagct aaagttgata
     7141 aatatgaaaa agcatacaga agaggtctaa tatctgatga agaaagatat gaaaaagtta
     7201 tagagacatg gacagaaaca actgataaag ttactgacgc tcttatgggt ggactagata
     7261 gactaaataa tatatatata atggcacatt caggagccag aggttctaaa aaccaaatta
     7321 gacagctagc aggtatgcgt ggtcttatgg cgaatgcatc tggtaaaaca gttgagatac
     7381 cagttaaatc taatttccgt gaaggtttat cagtattaga atactttaca tcttcacatg
     7441 gtgccagaaa aggtcttgca gatacagcta tacgtacagc tgaatctgga tacttaacaa
     7501 gaagacttgt tgatgtaagt caagatgtta ttgtaagaga agtagattgt ggaacagaag
     7561 atactacaga aatttatgct ataaaagaag gaaatgaagt tatagaagaa atatatgata
     7621 gaattgtagg aagatatact atagacccta tattaaatcc ggaaactgga gaagttatag
     7681 ttgaggctga ttcaatgata caagaagatg aagcagaaac tatagtagct ttaggcattg
     7741 aaaaaattag gataagaaca gttcttaact gtaaaactaa tcatggagtt tgttctaagt
     7801 gttatggtag aaacttggct acaggaaaag aagttaatat aggtgaagca gttggtataa
     7861 tagcagctca atctatcggt gaaccgggta ctcagcttac aatgcgtaca ttccatactg
     7921 gaggagttgc aggagctgac ataactcaag gtcttccaag ggttgaagaa ttatttgaag
     7981 ctagaaaacc aaaaggatta gctgtaataa ctgaagtatc tggtagagtt gaaattgatg
     8041 aaacaggaaa gagaaaagaa gtaaatgtaa taccagaaga aggcgagact caaacatatg
     8101 taattccata tggttctaga ttaaaagtta agcaaggtca aatgctagag gctggagacc
     8161 ctctaacaca aggatttata aatcctcatg atatcgtaag agtaaatggt gttaagggag
     8221 ttcaagaata tatagttaaa gaagttcaaa gagtgtatag acttcaaggg gttgatgtta
     8281 atgataagca tatagaagtt atagtaagac aaatgctatc taaagtaaaa gttgaagacc
     8341 caggagatac agatttattg ccaggtggat atgaagatgt attaacattc aatgaatgta
     8401 ataaagatgc tatagataaa ggtctaagac cagcagttgc taaaagagtt ttacttggta
     8461 taactaaagc atctcttgca actgattcat tcttatcagc agcttctttc caagaaacaa
     8521 caagagtatt aacagaggca gcaataaaag gtaaagaaga ccacttaata ggacttaaag
     8581 aaaatgttat attaggtaag ttaataccag caggaacagg aatgaagaaa tatagaaata
     8641 tagctgttga aaaaattgaa gattaatagc atatagagta aagaaatctt gacaaccatt
     8701 atttgtggtg ttaaaatagt aaagtgtgtt gtttaaaagt acactaaggg cttgaacaag
     8761 ctcttagtga cttttagatg gcaataaata agaactaaaa agcatcaagc tagaaaagtg
     8821 ctaatatcac atatttatgt gttatagaca ttaaattgta agtaacgaat gttacaatca
     8881 gtcagtatga ataattcata ttgatgtagt tggcggtcac tgataagatc gaaaatttgg
     8941 aaggaggtgc agatgatgcc aacaattaac caattagttc gtaaaagtag aaaagcgcta
     9001 gagaagaaat ctactgctcc agcattacaa aaaggttaca actctttaaa taagaaagta
     9061 actgacgcta gtgccccaca aaaaagaggg gtttgtactt cagtaaaaac agttactcct
     9121 aagaagccta actcagcttt aagaaaagtt gccagagtta gattaactaa tggtatagaa
     9181 gtttctgctt atataccagg agaaggacac aacttacaag agcatagtgt tgttcttata
     9241 agaggaggaa gagtaaaaga ccttccaggg gttagatacc atatactaag aggtactcta
     9301 gatacagcag gtgttgataa gagaagacaa tcaagatcta aatacggtgc gaagagacct
     9361 aaagaagcta aaaaataaaa caattaatga ttgattatag ttcggtgctt taaatatata
     9421 tatatttacg gcatcacggc attaaatgat ttatgtcgag tacctatgat ttaagttaaa
     9481 taattaagga gggaagcgaa atgccaagaa aaggtaatat tccaaaaaga gaagttttac
     9541 cagatcctat gtatgggagt aaggtagtta ctaaattaat aaataatcta atggtagacg
     9601 gaaaaaaagg aaagtctcaa agaatagttt atgatgcttt cgcaattgtt gctgaaaaga
     9661 caggagaaga agctttagaa gtatttaata aagcaatgga taatataatg cctgttttag
     9721 aagtaaaagc aagaagagtt ggtggagcaa actaccaagt gccaatcgaa gttagacctg
     9781 aaagaagaca aacattaggt ttaagatggc tagtaaaata tactagagct cgtggtgaaa
     9841 aaggaatggt tgaaaagcta gctaaagaga taatggatgc agctaacaac actggagctt
     9901 cagttaagaa aaaagaagat actcataaaa tggcagaagc taataaagca tttgctcatt
     9961 acagatggta ggatatatag atatcttata aattcaagga tatcataagg aggataacca
    10021 tggctagaaa gtttcctttg gaaaaaacta ggaatatagg aatcatggct catatagatg
    10081 caggaaagac tactactaca gaaagaatcc tgttttatac tgggcaaact cataaaatag
    10141 gagaaactca tgaaggagct tctcaaatgg actggatgga gcaagaaaag gaaagaggta
    10201 taacaataac ttctgccgct actactgctt catggaaaga ccatagaata aacataatag
    10261 atactcctgg acacgtggac ttcacagttg aggttgaaag atctctaaga gttcttgatg
    10321 gttcagttgc tgtattctgt gcaaaaggtg gggttgagcc tcaatctgaa aatgtatgga
    10381 gacaagctga aacttatgga gtacctagaa tagcttttat aaacaaaatg gatatattag
    10441 gtgcagattt ctacaatgtt gtaagcatga tgaaatctag attaaattct aatgcagttc
    10501 ctatgcaatt accaataggt aaagaagatt catttatagg tataatagac ttattaaaaa
    10561 tggatgctgt tatatacaaa gacgacttag gggttgaaat ggaagaaaca gacataccag
    10621 aagatatgaa agaattagct gctgagtgga gagaaaaatt agttgagtca gtagctgaaa
    10681 ctgatgaaga attaatgatg aaataccttg aaggtgaaga acttactata gatgaattaa
    10741 aagttgctat aagaaaagct actatagctt gtgaaatgaa cccagttttc tgtggtacag
    10801 cttatagaaa caaaggtgtt cagttagtaa tagatgcagt tttagattac ttaccagcac
    10861 caactgatat accagctata aaaggtatat tagcagatgg agaagaagct gaaagacatt
    10921 cttctgatga agagccattc tctgcattag cattcaaaat aatgactgac ccattcgttg
    10981 gaaaactagc gttcttcaga gtttattctg gaacattaga atctggttct tatgttttaa
    11041 atgcaactaa gaataagaga gaaagaattg gtcgtatatt acaaatgcat gccaatacaa
    11101 gagaagaaat aactaaagtt tatgctggag atatagcagc agcggtagga ttaaaagata
    11161 ctactactgg agacacatta tgtgaccctg ctaatccaat aatacttgaa tctatggaat
    11221 tccctgagcc agttatatct gttgctatag agccaagctc taaagcagca caagagaaaa
    11281 tgggtatagc tcttcaaaaa ctagctgaag aagacccaac tttcacagtt aagactgacc
    11341 aagaaacagg acaaactata atatctggta tgggtgaatt acacttagag ataatagtag
    11401 atagattatt aagagaattt aaagtagaag ctaaagttgg tgcaccacaa gttgcatata
    11461 gagagactat aactcaacca gttgatgttg aatacaagta ctctaagcaa tcaggtggta
    11521 gaggacaata cggtcacgtt aagattagag ttgctcctca agagccaggt gaaggataca
    11581 agttcacaaa caaaactgtt ggtggatctg taccaaaaga atatgttggt ccagttgaca
    11641 tgggtataca aggagctatg caatctggta tagttgctgg atatccagtt gtagacgttg
    11701 ctgttgagtt atatgatggt tcttaccatg aggttgactc ttctgagatg gcgttcaaga
    11761 tggctggttc aatggcattc aaagatgcta tgaagaaagg taatgcagtt cttttagagc
    11821 cttacttcaa agtagaagtt gttactccag aagattacat gggagatgtt atgggtgact
    11881 taaactcaag aagaggatta atccaaggta tggaagctag aagtggtgca caagttataa
    11941 atgcattcgt tccactttca gaaatgtttg gatactcaac agacctaaga tcttcaactc
    12001 aaggtcgtgc aacttacaca atgatattcg atcactatga gcaagttcca gcttcagttg
    12061 ctaagaaaat agctgaagga aaataattta aaaataatta ataatataaa tataaaaaat
    12121 aatcagctag atgagatata tctcatctag cacaagat
//
LOCUS       DAMJVM010000106        12129 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.110, whole genome shotgun sequence.
ACCESSION   DAMJVM010000106 DAMJVM010000000
VERSION     DAMJVM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12129
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.110"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            329..1528
                     /locus_tag="PC523_002980"
     CDS             329..1528
                     /locus_tag="PC523_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HDF2476480.1"
                     /translation="MEKLYYTDQYIKDFTAEIVEIKEVENKFHVLLDKTAFFPGGGGQ
                     FADLGKIDNYDVISVYEEGKKVYHVLDKKPIKIHRVKCSIDWNRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGSEYSTVDIEGNLDEDKIREIELLSNEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVVKIGDLDTNACCGVHPKATGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDDILKRDIYLTKICRYLSCNEEDAINGIVNLNSKVEEVLIKKRRLE
                     EVVSTYQVKEMIEKANKIGNISVVKKIYEDEDLKYVNKIANKITEHENNIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKILAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            1580..2080
                     /locus_tag="PC523_002981"
     CDS             1580..2080
                     /locus_tag="PC523_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2476481.1"
                     /translation="MEQQKDYIELYENEELIEIREACMNDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENMSAKATYESLNMYECDYNMY
                     EYEVMR"
     gene            2182..2499
                     /locus_tag="PC523_002982"
     CDS             2182..2499
                     /locus_tag="PC523_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476482.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASIFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     IIQ"
     gene            2657..3616
                     /locus_tag="PC523_002983"
     CDS             2657..3616
                     /locus_tag="PC523_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2476483.1"
                     /translation="MGESKKSENLLLSIIYIIFFVYILMIIRIILFRDVPIYAISKGT
                     FRTVNLIPFYTIYQFIVDSNIDFMKATINIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     TIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGITIYINMNKLFPNDIKRFKVII
                     ATSLILGVIGLGVISKNYHNLLTFKFRPDKKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            3731..4516
                     /locus_tag="PC523_002984"
     CDS             3731..4516
                     /locus_tag="PC523_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HDF2476484.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGIMSVLIIRFIHPFIKDFVGIIPYRILISMSIITTLWAILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            4808..6127
                     /locus_tag="PC523_002985"
     CDS             4808..6127
                     /locus_tag="PC523_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2476485.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(6201..6635)
                     /locus_tag="PC523_002986"
     CDS             complement(6201..6635)
                     /locus_tag="PC523_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476486.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RRKDDKKKGNKKRNTNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAIL
                     IITKLTDSTNKGTYSRNTVSNTHKNDIRNVVFNESNNFLICG"
     gene            complement(6726..8102)
                     /gene="hydG"
                     /locus_tag="PC523_002987"
     CDS             complement(6726..8102)
                     /gene="hydG"
                     /locus_tag="PC523_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HDF2476487.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLISEEIDKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(8103..9188)
                     /gene="hydE"
                     /locus_tag="PC523_002988"
     CDS             complement(8103..9188)
                     /gene="hydE"
                     /locus_tag="PC523_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HDF2476488.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDTYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(9224..10432)
                     /gene="hydF"
                     /locus_tag="PC523_002989"
     CDS             complement(9224..10432)
                     /gene="hydF"
                     /locus_tag="PC523_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HDF2476489.1"
                     /translation="MSLNSTPQSIRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGKLGELRISKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLAKNKVKCPVVSVSSTDKIG
                     IENLKNEIIRVLPKDGTEFRLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKENSLKETLSNLGKKPKLVITDSQVFPQVDKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALETLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGILIAYVKGI
                     LERSLELFNY"
     gene            complement(10447..10698)
                     /locus_tag="PC523_002990"
     CDS             complement(10447..10698)
                     /locus_tag="PC523_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HDF2476490.1"
                     /translation="MKKVAVISAILEEPKECQLKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDIINSLTGKLGNIPHVSVKTSISKKEI"
     gene            complement(11079..12119)
                     /locus_tag="PC523_002991"
     CDS             complement(11079..12119)
                     /locus_tag="PC523_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDF2476491.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLECKKDFEDLGLDTL
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAMVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVVTRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFETTESGFIKSRYIDNKSAVAMVLEICRYFKENNLAPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VNVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT         4446 a         1521 c         1831 g         4331 t
ORIGIN      
        1 gaatattaat gtgataaaca tatattgaaa attattaata atagatgaga ttagtaaaag
       61 tgattaaatt taatttgtag gtttaatata tttcggtaaa ttttttatat tgtaagaaga
      121 catggaagca tatacatttg taaaatatcg aaataatata cttataaatt tgttaacact
      181 aatatctatg tgttaaaata tagtataaga ttgttagcaa agaatgggat tagttctatc
      241 ctagtacaat taaattaaag ttatactaag atttgtatat ttaaaaacta aagtagttta
      301 gaactaagga aatggagata gaattataat ggaaaaatta tactatacag accagtatat
      361 aaaagatttt acagcagaaa tagttgaaat aaaagaagta gaaaataagt ttcatgtttt
      421 gttggataag acagcgtttt tccctggtgg tggaggtcag tttgcagact taggaaaaat
      481 agataactat gatgtaataa gtgtatatga agaggggaaa aaagtatatc atgtattaga
      541 taaaaaacca ataaagatac atagagtaaa atgcagtatt gattggaata gaagagaaga
      601 tggtatgcat caacactttg ggcaacatgt actgtcaggt tgttttttta agactttcaa
      661 tgcaaataca acaggttttc atctaggaag tgagtatagt acagtagata tagaagggaa
      721 tttagatgag gataaaatta gagagataga gttgctttct aatgaaatta ttcgtcaaaa
      781 tatacaagta gagacgctac ttccaacaaa aaaagaattg aaaaagattt ggttaagacg
      841 tgatttgcca aatactgatg aagaaataag agtagtaaaa ataggagatt tagatacgaa
      901 tgcatgttgt ggagtacatc caaaagcgac tggtgaattg agaatgataa aaataaagaa
      961 atgggaaaaa cataaatcta gtacaagaat agagtttttg gcaggtaaaa gggcagtaga
     1021 tgatatatta aaaagagata tatatttaac taaaatatgc agatacttaa gttgtaatga
     1081 agaagatgct ataaatggta ttgtaaatct aaatagtaaa gtggaagagg ttctaattaa
     1141 aaaaagaaga cttgaagaag tagtgtctac atatcaagtc aaagaaatga ttgaaaaagc
     1201 aaataagatt ggcaatattt cagtcgttaa aaaaatttat gaggatgaag atttgaaata
     1261 tgtaaataag atagcaaata aaattacaga acatgaaaat aatattgcat tattagctgt
     1321 taaatttaat gataaaataa atttagtttt tgcatgttct aaaaatttaa aagatattaa
     1381 tatgggagtt ttattaaagg atgcaataag ccttatagat ggtaaaggtg gtggaagtaa
     1441 aatattagct caaggaggag gtaagaataa tggaaattta gaagctacac ttgactatgc
     1501 atttatgaag atagaaaaaa ctctataaat atattatata aaaaatctta atagataaaa
     1561 tagatatgga ggaataagta tggagcagca aaaagattac atagagttat atgaaaatga
     1621 agaattaata gagataagag aagcctgtat gaatgatttg gatacaatag caaaatttaa
     1681 ttataatttg gccaaagaaa cagaaggaaa agaacttgac atggatgttt taactaaagg
     1741 tgtaaaagca ttgttattag atgagagaaa aggaaaatat catgtatata cagtatttga
     1801 taaggttgtt gcacagatta tgtatacata tgagtggagt gattggagaa atggaaattt
     1861 cctttggatt caaagtgtat atgtagataa ggaatataga agaaaaggta tctttaatta
     1921 tttatttaat tatataaaaa atatatgtga taaagatgaa aatattgttg gaatgagatt
     1981 atatgtagaa aaagaaaaca tgagtgcaaa agcaacatat gagtctttaa acatgtacga
     2041 gtgtgattat aatatgtatg aatacgaggt aatgcgttag taatataaat ttataatatt
     2101 aatattataa agtattgatt atattgacaa ttatgtggaa taaattggat attataatta
     2161 ggaattttag ggggtaaaaa aatgggcgtt ttaaaaggaa tgttttatgt atttttattt
     2221 ttagacttag tatcaatatt ttgtttcttt tttaataaag gaaaaatagc atctaataaa
     2281 attgtgttta atgcaatagg agtacttaca tttgtacttt gttttatgct ttttagttat
     2341 tatcctaata ataatcttat tggaaaattt attgcatcta tattttttat atttggagtt
     2401 gcaggagtta tgttgaaaga aaaaaatttt ttatatgcta gactcctttt aactgtagtt
     2461 attgtttttt ctacactaag attgttcatt attcaataaa atacatttgt atgaagtttt
     2521 gttaaagtct gcacatttaa gtgtaggctt ttttgtattt ttatttagaa gttaagggaa
     2581 ttttaagaaa attttaaaat cgtcttaaat atttattggt acacttgaaa tattaataga
     2641 tagaggaggc ttatcagtgg gagaaagtaa aaagtcagag aatttgttac tgtcaattat
     2701 atacataata ttttttgtat atattttaat gattataagg ataattttat ttagagatgt
     2761 accaatttat gctatttcca agggaacatt taggactgta aatttaattc cattttatac
     2821 aatatatcaa tttatagtag atagtaatat tgactttatg aaagctacta taaatattat
     2881 agaaaatata ggtatattta ttcctatggg aattttttta cctatagtat gtaaaaatct
     2941 aaataaaaaa actataatta taacaattat acttgttagc ttagcatttg aattaacaca
     3001 atatattttt gctcttggaa gtagtgatat agatgatgtt attttaaatt cattaggtgg
     3061 cataattgga attactattt acataaacat gaataaatta tttcctaatg atattaaaag
     3121 atttaaggtt ataatagcta ctagtttaat tttaggagtt ataggattag gagttatttc
     3181 taaaaattat cataacttac taacttttaa atttagacct gataaaaaaa tatcaaaaat
     3241 attaattgaa gaaaataggg aaataattaa ggatataaat aaagatacag tagacatagt
     3301 tgggacattt gagagcttta aaaaaggaat aataactatt aaagcaggct ctaataacaa
     3361 agtaaagaga ccagagaatt taattgattc tgatggaaat ataagaattt atttaaatga
     3421 aaatacaaaa ttagtatctt atataattac caaagaatca aaaatggata tagttaaata
     3481 tgaagaattt gatgtgaaga atttagattt attaaggaga tatgatacta ttaatgtttg
     3541 gattgataaa gaaaatcagc caaaggataa acatggtatt atggctagta aattattaat
     3601 tggattgtat gaataaaata ttaaacaaga ataaaagaat aacaaatata aaaaacgaaa
     3661 aaactatttc tataattatt tttacgttgt gcataaaata agaacactgg tgaaggagta
     3721 gagataaatt atggagataa taaaatatat cttgtacttt tttatatatt catttttagg
     3781 atggacagta gaaagtatag gatgttctat agcaagcaaa agaataatca acagaggttt
     3841 tttaaatggt ccaatatgcc ctgtttatgg ttttggagcg gtaattgtga tatcactatt
     3901 gggaagattt aataatgtag taatagtatt tctattagga atgattttaa ctacaatact
     3961 agaatatttt acaggattta ttttagaaac cttatttcat gctaaatggt gggattattc
     4021 tgacagaaaa ttcaatataa agggtagagt atgtttaaaa aatgcaatat actttggtat
     4081 aatgtctgtt ttaataataa gatttataca tccatttata aaggattttg taggtataat
     4141 tccatataga atattaattt caatgtctat aattacaaca ctttgggcta tacttgattt
     4201 gattgttact attataacac ttaaaaaatt agatataaaa ttaaacttac tagacgatat
     4261 aattacagac cttaatgata taaatgtgaa attggataag tttgatagag gagaaataca
     4321 agctttattt aaaacaataa ataacgacga attagaagtt agagaaaaaa taaacaaaat
     4381 aaatagtaag ctggaccgta tagagtctaa tattatttta caaaaaagag ttataaaagc
     4441 ttttccacat ataaaacata aaaaacaaca agaacaacta gaacatttta aaaaaattat
     4501 taatgagaaa aggtaattat acaatttaat gaaaaagaat agaataatgg tatttataag
     4561 atgattttca gagggctcta taaaaataga atagagtcct ttattttttg aaaaataatg
     4621 tttatttgat aaaaataaaa tatattgttt ctgaataaca aatattttga aaattataaa
     4681 aacatatatt gacaatttgt gtcaaaaata ctaacattaa gtatataaag aattttatat
     4741 gagaatatct ttataaatcg caataatttt aaaacataaa tttatttaaa gaggggatga
     4801 tttgccaatg agcgagataa ctagtcaaat gataagtaca gaagaaaaaa aatatgttgc
     4861 taaaactcaa aagatacctt attatccagt agcatttaaa tcaggtgatg gtgcaatgct
     4921 atatgattat gaaggtaatg aatatgtaga ttttttagca agtgcaggct ctgcaaatgt
     4981 aggtcatggt aataaggaaa tctcacaagc agttaaagaa caaatggatg atataacaca
     5041 atatacactt gcatatttcc atagtgatcc tcctgtaaaa ttagctgaga agcttgtgga
     5101 aatagcccct ggagataatg ataaaaaagt actatacagt gcaactggtt ctgcttgtat
     5161 agatgctgct ataaaattag caagagggta tactggaaga acaaagataa tatctatgtg
     5221 tgaatcttat catggaagca cttatggagc catatctata tctgctttaa gtacaaatat
     5281 gagaagaaaa atgggtccat tgttaccaga agtttatcac ttccattatc ctgataaaaa
     5341 tagaacagct aaagaatgtt tagatgagat agaatatgct tttgcacact atttaccagc
     5401 agaagaagta gctgcaatat ttatagaacc aattgctgga gatgcaggaa taatagtgcc
     5461 tccagtagag tgggtacaag gtttaagtaa aatatgtaaa gaaaatggga tattacttgt
     5521 aagtgatgaa atacaacaag gtatgggaag aacaggtaag tggtttggta tagaaaattt
     5581 tggtgttgaa gcagacctta tagttcttgg aaaatctgtt ggaggtggat tacctctagg
     5641 agcagttgtt ggaagaacag aaataatgca aagtcttgat gcaccagcac atttatttac
     5701 tttagctgga aatacaacag tatgtgtggc tgctttaaaa tctatagaga taattgaaaa
     5761 agaaaactta cttcaaaaga gtatagaaat gggagattat attaaagcag gatttgaaaa
     5821 actaaaagaa aaatatgata taattggaga aataagaggt ataggattat ctataggagt
     5881 tgatatagtt aaaggaaaag gttctaatga aaaacatcca gatgctacag ctaagatatg
     5941 ttatagatgt atacaaactg gattaataat gatattctta ggacaatcta ctttaagagt
     6001 gcaacctcct ctagtaataa ctaaggagca agtagataaa gcaatgaaca tcatagattc
     6061 tgcaatagat gattatttaa atggtagaat tggtgatgaa gtatatgaag taactcaagg
     6121 ttggtaaaat aaatattaaa taaaataaga gttatctcaa ttttagaaag taatttctga
     6181 aatgagataa ctcttttaag ttacccacaa ataagaaaat tgtttgattc attaaagact
     6241 acatttcgga tatcgttttt gtgagtatta gagacagtat tgcgagaata agtaccttta
     6301 ttcgtactat ctgttaattt agttataatt aagatagcta aaagaataag aattactgtg
     6361 tataaagcaa taacgtatgg ttttctttta tatttgtatt tattattaaa aaaagaatga
     6421 ccataattat ttttatttac tttagatgta tttgtatttg tattcctttt cttgttacct
     6481 ttctttttat catcctttct tctattttta gggtaaggaa tgactttgtg tgtattatca
     6541 agtttgatat ttttattttt ttgaatattg ttttccaggc tagccttttt ttctccaaat
     6601 tgaatagttt tattggtatt gttgttatct ttcaatgtta atctccctcc cttaaacttt
     6661 ccctttagat aatattacta ctaacctatg gaatatacaa taaataatat ataagtaata
     6721 aaattttata ggtataaatc tctttctcca cgctctaatg cagtcatatt gttagatact
     6781 atgtttctta tatcttctct ttctatctta tctatttctt ctgagataag ttttttaccc
     6841 ataattttaa gttcttcatc tccataatcg attagaaatt catttaatgt agtaagagca
     6901 tttggtgtac atacattatg aatttgtcga cttttagcta agctcataaa tctatcgcca
     6961 gttctaccct tgcgataaca agctgtacaa tagctaggta tatatccatc agtaattagt
     7021 tctttaagaa cctctacagg gcttctatgg tctccaactt cgaattgttc tgtattgtca
     7081 ccatcttcat atgctttata tcccccaact cctgttagag aacctgcact aatttgtgat
     7141 acaccatatt ttagtaactc atttctcatt tcagcagttt ctctagtgga cattatgatt
     7201 ccagtaaaag gaacagctaa tctagttata gctactattt ttttgaacat atcatcagaa
     7261 actaagtgag gaaaatcttc taagctcata ccttcagctt ttttcaatct aggaaaagat
     7321 atagtatgga aacctacacc aaatttttcc tctaagtgtt cgttatgcat cataagccct
     7381 agaacttcaa atttagggtc agataaacca aataaaactc ctgcaccaac atcatcaacg
     7441 ccagcttcca tagctctatc aaaagctgtt aaatggtaat aatagtcatt ttttatagat
     7501 tgaccatgca tttttataaa tgttggtttg tgataagttt cttggaataa tatataagtt
     7561 cctattcctt tttcttttag taatttatac tcatctacac ttgtagctgc tatgttaacg
     7621 tttacccttc ttatatttcc attttcgtta tatgttgaat aaattgcatc taaacaatct
     7681 aagatataat ttatatcaca atttttaggg tcttcaccag cttctagagc aagtctttta
     7741 tgacccattt tttctaatat tttaacctct tcctttatct cttccatggt aagttttttt
     7801 cttttaaatt tgttgcattt gctaaaaccg caataaacac attcatttac acagtagttt
     7861 gatacataaa gaggggcaaa taaaaccact ctattaccat atatttcatt ttttatttgc
     7921 ccagctatac taaataatct atctagttgt tttttatctt ctacttcaag aagtgtagct
     7981 atatctttat aactaagttt ttcccttcta tcagccttat caagtacctt ttcaatatca
     8041 tcagaagttg aattttttgc atcttctaat aaagaattaa taagttcatg gtttatgaac
     8101 atttacaaac gcctccattc aatattatca ccacgagaat agtcgacttc aagtcctaat
     8161 gacttgatgt tttcttctaa gctttggtga tattcacaag cttcttgacc agtaaaggct
     8221 ttgttatcgt aaagagaata ttgttttcta aattcttgag gagaaaggtt tggcataatt
     8281 acattgcaac cagctaaaag acctgcattt ctaccactag ggtttataga tgccaaggct
     8341 gttgtagcag gtaataaaac ttttggaagt agaagtcttg taattgaaag cataagtaat
     8401 gtcttttcta aatctccatg tttataatcc ttaaatattg tatcatggtg tggaataaaa
     8461 ggtcctattc caaccatatg aggatttaac tcttttaggt ataataaatc acgcacaagg
     8521 tcctcattgt tctgaaatgg tgactcaacc ataaatccag cacctacttg ataaccaatt
     8581 tccttaaggt tttttaggca ttcttttcta gttctaagct caatgtttgg tggatgtaat
     8641 tttttataat gactgtctgt agcagtttca tgtcttaaaa gatatctatc tgcaccacat
     8701 aaaaagtatt ttttatacga atcataactc ttttctccta gtgatagagt tatggcacaa
     8761 tcctcatatt tattttttat acttgaaaca atttcacata ttttatcatc agtaaaatat
     8821 gtatcctcac caccttgcaa aacaaatgtt ctatatccta ttttataacc tatatcacaa
     8881 cattcaagta tttcatcaag tgaaagtctg tatcttatga tatttttatt agaagattgt
     8941 attccacaat agtagcaatt atttttacaa tagtttgtaa gctctataag tcctctcaag
     9001 tagactctat ttccgtaatg tttatttctc atgtcgctag cttgtttata aagatagttt
     9061 cttaaatttt gttcgaagtt atattcttca ttatcgatat tttctattaa atatagtagt
     9121 tcatcaaaac ttaatgaatt aatgctgtaa agtttatcta taaagtattt aatcttattt
     9181 ttagtcatat tcaaatatgt aattattaaa tatagttaaa tttttaataa ttgaaaagtt
     9241 caagtgatct ttctaaaata ccttttacat atgctattaa aataccatag tttacaatcg
     9301 gtacattgaa agattttgcc ttttctattc tagataacat tccagctcta ttcatcatac
     9361 aagctccaca gtggactaca agggcatatt tatttatatc ttcagtgaag gatactccag
     9421 aactaaattc aaaagtaata tttttgccag ttttctttct aatcatatta ggaattttta
     9481 ccgttccaat atcatcagtt tgtctatgat gggtacatcc ttcggccatc aagattttat
     9541 caccatcttt taaagtttct aaagcataag ctccattaat caactcttta aggtctcctt
     9601 tttgtctagc aaataatata gaaaaagatg taagaggaat atcttttggt gtatctttat
     9661 ctacttgtgg aaatacttga gagtcagtaa taacaagttt cggttttttg cctaaattag
     9721 atagagtttc ttttaaacta ttttcttttg taactataga aattgcacca ttatccaaaa
     9781 tatctcttat aacttgttgt tgaggaagta taagtctacc ttttggagct gctttatcta
     9841 ttggaacaac taaaacaact aaatcatttg gttcaattaa atccgatacc aatctgaact
     9901 cagtgccatc tttaggcaaa acccttatta tttcattttt taaattttca atcccaattt
     9961 tatctgttga tgagacagaa acaactggac atttaacctt atttttagct aagtttaaaa
    10021 tttctgattg attcttaata gtatcaattt tgttaagaat taaaatatga ggtatattct
    10081 tatcattaaa cttttcaatt agtgataaat cttcttgtga tataccaatt tggcaatcca
    10141 ctactagtaa agctatatct gtcttttcta aaacatctag tgatttactt attctaagtt
    10201 caccaagttt accaacatca tcaagaccag cagtatctat aagaacacaa ggtcctatag
    10261 gaaggatttc cattggtcta aatactgggt ctgtagtagt tccagcaata tctgatacta
    10321 ttgcagcgct ttgatttgtt atagcattta ttatgctaga tttacctgca tttcttttac
    10381 caaagagacc tatatgcact cttattgatt gtggagtaga atttagactc atttgtactc
    10441 accaccttaa atttcttttt ttgatatact agtttttaca gatacatgag gtatgttgcc
    10501 caatttacca gtcaaactat taataatatc catttcgcca acaactgcta tacatacgat
    10561 tgatacacct tcttcttcgc aaggaatgcc cattcttccc ttaataatac ctttaaaacc
    10621 tgatacaacc tcattgaatt taagctgaca ttcttttggc tcttccaaaa tagcacttat
    10681 aactgcaacc tttttcataa atatcctcct aactcagaaa atatgtctta aacaaatcca
    10741 aaatcaaaaa acttttcttt gtaaagaaaa gtttctacaa aagaaaagtt attaattaca
    10801 caagtataat gtgctatatt aaactatttc cttacagatt agataactct tttctggtag
    10861 ggttagcttg taaaaattta atttaacaac aattaactga agaatatttt tatacaattt
    10921 atataaatta taataaaaat taactaaaga aaattgaagt tacttatata atagtagtaa
    10981 attgaaatta agtctataga aaaaccaatt tttgtataaa aataaaaatt aaagctagtt
    11041 atttgttaag aattgacttt ggtaaaatat atatttaatt attctttaag catataattt
    11101 ataagtaatt ttagtgtatt ttcaatagct tctatatgtg ttctttcata atggtgacta
    11161 ttgtttgttc caggaccaac acatgctatt tgaacatctt ctccccaacg aatagcttct
    11221 gaagcatctg aaccatagaa agtaaataca tcgacagcgt agtctaaatt attgtcctct
    11281 gcaacattca caagcttgtt tcttaagtta aaatcatata atgataaatt atcctttgca
    11341 actatagtta caccatgttc actagatgtc tgacttggtc cagtaggtgc tatatctatt
    11401 gctacaaatt cttttgtttt ttcaggtaaa catttagaaa caccatgtcc aatttcttca
    11461 taattgctta tataaaagtt agtagtatgc ttaggtgcta aattattttc tttaaaatat
    11521 ctacatattt caagaaccat tgctacagct gatttattat ctatatatct agattttata
    11581 aaaccagatt ctgtagtctc aaatcgatgg tctagacata caaaatcccc aacacttatt
    11641 cctaagtcta atacatcttg ctttgtaaat actttttcat caagtctaat aatcattgtt
    11701 tcttcatttc tttcagtagc ggctttggtt tgaccatata tatgagtaga agcatactta
    11761 aatacaacac ttcctctgat ttttttacct tttctagtta caaccgtaca gttttctcct
    11821 tctatagcag cccaacatcc accaccaact ttatggtatt taagagtacc atcagaggat
    11881 atttctttta ccatagctcc aagtgtatcc atatgtgcag atattgttat ttgatttgaa
    11941 tcatcttcac caggaagagt ggctataagt gcaccttttt ttgtaagaag agtgtcaaga
    12001 cctaaatctt caaaatcttt tttgcattct aaaactgcat tttcagtata tccagctggg
    12061 cttggaatgt ccaaatattt tttcattaaa tttatagtat tagttaaatt taaattcata
    12121 ttgtttcct
//
LOCUS       DAMJVM010000107        11910 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.111, whole genome shotgun sequence.
ACCESSION   DAMJVM010000107 DAMJVM010000000
VERSION     DAMJVM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11910
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.111"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(210..362)
                     /locus_tag="PC523_002992"
     CDS             complement(210..362)
                     /locus_tag="PC523_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDF2476492.1"
                     /translation="MATFIIAAIVVVLMALAVMYMIKNLKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            complement(388..2544)
                     /gene="feoB"
                     /locus_tag="PC523_002993"
     CDS             complement(388..2544)
                     /gene="feoB"
                     /locus_tag="PC523_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2476493.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKEAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISNIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKVPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSIKGVLIHM
                     WDRGKAFIVKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVANAFAFMAF
                     NMLCAPCFAAIGSIKREMGSWKWTWITLGFQTLTAYIVALLINQVGSLVLGTGGSIAG
                     AIISVVIAVGVVFAVLTYSNRNMKKEKMGKLSYMKN"
     gene            complement(2647..2868)
                     /locus_tag="PC523_002994"
     CDS             complement(2647..2868)
                     /locus_tag="PC523_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2476494.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEITVRDYELSIRKSDAEKIIVE"
     gene            complement(2881..3093)
                     /locus_tag="PC523_002995"
     CDS             complement(2881..3093)
                     /locus_tag="PC523_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2476495.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            complement(3414..6311)
                     /locus_tag="PC523_002996"
     CDS             complement(3414..6311)
                     /locus_tag="PC523_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2476496.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIKVGYCD
                     DYGIISSSKEHSYSGYGYDYLREISKYTRWEYEFIKGSWEECLDRLEKGEIDLLGPLQ
                     KDDERNKIFNFPKLSSGYEYSALYTDDSNNNMFYEDINSFNGMRVAVLRGNFHNIAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIIDAKGMKIVSKFSVEPFYFA
                     TAKDKPNLAKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEINFIKANPK
                     LTMVYDSEWSPVEYYDKKSNSFKGISSDLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKATMICGSINENDKAKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYNSVESCLNAINEGKANLMVLSSYQFDELLRKGKSENLSVISVL
                     DTSYGMRIGVSNKTNPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLKRIAYTDSLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYMDVDKFKYINDMFGYDMGNNTLIHISDTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNSIYNKVQIFSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFVVYFQPKYRLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQE
                     VVPISVNLSRMHFVNSNFIEKFKLIVDKYKIPTSLIELELTETAVLDNIEGLLDTMNN
                     LKENGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            complement(6637..7245)
                     /locus_tag="PC523_002997"
     CDS             complement(6637..7245)
                     /locus_tag="PC523_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDF2476497.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFIAGYSVGA
                     TIAWRCSEDSLCDGIICCYGSRIRDYMDVIPKCPTLLVFAKYDSFDVNSISCQLSRKQ
                     NIQIEILDANHGFIDIYSKNYSDIQTKAFTVKEKSFLSKCLV"
     gene            complement(7505..8050)
                     /locus_tag="PC523_002998"
     CDS             complement(7505..8050)
                     /locus_tag="PC523_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HDF2476498.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(8168..9991)
                     /locus_tag="PC523_002999"
     CDS             complement(8168..9991)
                     /locus_tag="PC523_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2476499.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKSLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPTVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYEIKSVNDFKEINDRLLKISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGVLASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEKEEQELDNMFKSELKDNKDGNSNSHFVKGEIELKNVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDSKDIYSIDRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANSHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(10004..11779)
                     /locus_tag="PC523_003000"
     CDS             complement(10004..11779)
                     /locus_tag="PC523_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2476500.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGVIMIGITFLGAIFAIIRSVISARVSQHFSYYLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKVGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENKSF
                     EELNYELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRYLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLIDEDEEVIERDKSSDIGEKDLKFA
                     NCDIIFENVDFAYNKNTGEKILKNISFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGHSIYSISEESLRERIGVVFQNSSVFGKSILENVVMGNRNIRKEDVIKSC
                     EIANANQFITKLENSYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKDHISNLRENEGKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFTLQNLVSEDVN"
BASE COUNT         3977 a         2148 c         1085 g         4700 t
ORIGIN      
        1 gtagctattt tttaatcaaa tttaaacata atatttactc atattgctta atttagcaat
       61 aatccctcct aatcatatat ttatttataa ttatacagta aaattacaac atagtctaac
      121 ttacttcaaa tatgaaattt gttatttata taaaaaataa gaacctgata ataaagtaat
      181 ctaaactttt tatcaggctc ttatcctatt tatctcttag cactatggca actcttacta
      241 cttgagcatc cagaacaacc acatccacaa ctgctaccat tcttttttaa atttttaatc
      301 atatacatta cagccaatgc cattaatact actacaatag ctgctattat aaatgtagcc
      361 atatttatca ctccttattt atatgaatta atttttcata taacttaatt tacccatttt
      421 ttcttttttc atatttctat ttgaataagt taaaactgca aataccactc ctactgctat
      481 aactacagat attattgccc cagcaatact tcctccagta cctaaaacta aacttccaac
      541 ttgattgata agaagagcaa ctatatatgc tgttaaagtc tgaaatccta atgtaatcca
      601 tgtccacttc catgaaccca tttctctttt tatagaccca atagctgcaa aacaaggtgc
      661 acaaagcata ttaaacgcca taaatgcaaa cgcattagct actgtaaaca tacttgcaac
      721 tgaagcaaga agtgttgggt cttgctctgt tgcatctgat attccaaata aaacaccaaa
      781 tgttccaact acattttctt tagctactag acctgtaact gttgctactg atgattgcca
      841 atttccaaat ccaagaggcg caaatattgg tgcaactata tttcctaagc tagctaatat
      901 actatctcca gcatctacca tttgtaatga ccaattaaat gattgtaaga accaaatcac
      961 tccacaagca acaaatataa ttgttcctgc tttgactata aatgcttttc ctctatccca
     1021 catatgtatt aaaacacctt ttatacttgg aatatgatat tgaggtaatt ccataacaaa
     1081 tggagatggt tctcctgcaa ataaactagt tttctttaat ataattccac aaatgattat
     1141 cattgcaata cctaagaaat acattgatgg agctacccat gaagcacctc caaataatgc
     1201 ccctgcaaat aatgctatta ttggaacctt agctccacat ggaatgaatg tagttaacat
     1261 aatagtcatt tttctatctc tatcattctc tatagtacga gttgacataa ctcctggaac
     1321 tccacaacct gaacttataa gcattggtat aaatgatttt cctgaaagac caaacttacg
     1381 gaaaattcta tccattataa aagctacacg tgacatatac ccacaatctt ctaatattga
     1441 taataataag aataaaagca tgatttgtgg tacaaatcct aatactgctc caactccacc
     1501 aattaaacca tcaactacaa gtccttgcaa ccactctgct acatttaaag aagctagaaa
     1561 attacttaca ttaccttgaa taatttctcc aaacagtaca tcatttgtcc aatcagttgc
     1621 tatagtaccc aatgaactta ctgctatata gtaaactccc cacattataa gtgcaaaaat
     1681 aggtaatgct agtatacgat ttgttacaat cttatctatc ttatctgata cagtttcctt
     1741 accttttcta ttcttcttta ttatatttga aattattgaa ctaataaact cataacgatt
     1801 ggctgttatt atactttcac tatcatcatc caattcatct tcacaatttc tagttatttc
     1861 ttcaatgcta ttcaatattg ttttagaaat atcaagtttt tgtataacat tttcatctct
     1921 ttcaaataac tttatagcta accagcgtgt ttctacatct atatatggag tcttttcttc
     1981 tattattttt tcaatttttt ctattgcttc cttactttca tcaacgaaag gtaatttgaa
     2041 atttaattta ttgttactac ttgcaagttc aactgccttt tcagcagcct ccataattcc
     2101 ttgaccttta actgctgtta cttctaccac tgggcaacct ataacttcag atagttcttt
     2161 aatatttatc ttatcaccat tcttattaac aatatccatc atattaagag ctataacggt
     2221 cggtataccc agctctaaaa cttgagttgt aagatataaa tttctctcta tatttgatgc
     2281 gtctacaata tttattactg catccggctt atcatctaac ataaaattac gtgtaactac
     2341 ttcttctaat gtatatggag ataaagaata aatacctggt aaatctacta tctccacatc
     2401 tttatttcct tttaactttc cacctttttt ttcaactgtt actcctggcc agttcccaac
     2461 atattgtgat gaacctgtaa gaccattgaa cattgttgtt ttaccacagt taggatttcc
     2521 tatgagacct atttttattg acatttccat tccctccatt attatttaag tacataacta
     2581 ttcatgatac agaaaatcat tctcattata taatcgcact aaacctattc taactaatta
     2641 attttattat tccacaataa tcttttctgc atctgattta cgaattgata gttcataatc
     2701 tctgactgtt atctcaattg ggtcaccaag aggagctact tttcttataa atatattaac
     2761 acctcttgta atccccatat ccattatacg tcttcgtgta gctccttcgc cttctaactt
     2821 tttgactttt acagtctctc cacacttaat atcctttagc ctgttcatat tattacctcc
     2881 ctatattatt attctacttg ccattttttt atctaatgca actctagtat cttttacatc
     2941 tattattaag ttaccatcta tcttagaaac tatcttaaca tttgtaccta ctacaaaacc
     3001 tatactattt aaaaaagtcc tcgtcttatc acttcctgaa attttcttta taactacttc
     3061 ttcacctgta tttgataata tcaatggcat catatagtaa ccctcctctt aaactgattt
     3121 aaatccacaa gcaatcatca tctaatatat aaattacaat atcttaaatc ttaatctatt
     3181 aaaataatcc tctatttttt tccttctttt tacaactcct aattttgatt taagtctacc
     3241 acaatagtat gttttttgca agtgattatt gtattctttt gatttttttt ctcatttgat
     3301 tcattatatt aataaaacag taaaaattaa tacaaaaata aagatgctct aaaatagtat
     3361 actcaactat attaaagcac ctttattata tatgtcatta cttaaacatt gctttattct
     3421 tttttaaagg caattttttc aaactctttt acaggcattg gcttagaaaa caaatatcct
     3481 tgtaccatat cacaaccaat ttgctttaga aattctactt gcgaaatagt ctctactccc
     3541 tctgatatta ctttcatttt cagttccttt gccattttaa tgacattaga tataacaatt
     3601 ttttcattat tactctcatc tttttctgta aaaaatgctc tatctagttt taatatatca
     3661 acaggcaact cttttaaaag attaagagag gaatatcctg taccaaaatc gtccatagaa
     3721 attacaaatc cattttcttt caaattgttc attgtatcaa gtaaaccttc tatattatct
     3781 aatactgctg tttctgttag ttctaattct ataaggcttg tcggaatttt gtatttatct
     3841 acaattaact taaacttctc tataaaatta ctatttacaa aatgcattcg ggacaagttt
     3901 acagatatag gtactacttc ttgaccttca tccatccatt ctctaatctt tttacacact
     3961 tcctcaaata catagaaatc aatatttaca ataaaaccat ttctctcaaa tacaggaata
     4021 aattctatag gtggtattag acctttttgt ggattatccc atctaacaag agcctctgct
     4081 cctataatct gataatctga aagcctatat ttaggttgaa aataaacaac aaattctcca
     4141 ttattaagag catctaccat tgagttttca atttctttct cttttaatat ttttttatgc
     4201 atttccttat cgtaaaaagc aaatgaattt ttatgaccac cttttatagt ttttcttgca
     4261 gtattagccc tatccatgat agtgtttata tcattatcac ttttacttat tttatatata
     4321 ccacaatcta aaattaattt ataatagttt attttaggat tactaaatat ttgaacctta
     4381 ttataaatac tgtttaacct tgttttaata tcatcatctg tcttatactt aataagaaga
     4441 acaaaatggt cagcagaaac tcttgcaaaa atttcatctt cctttaattc acttgcaata
     4501 gtatctgata tatgtatcaa agtattattt cccatatcat atccaaacat atcatttata
     4561 tatttaaatt tgtcaacatc catataaaac aacgcatatt cttctggatt attcttagca
     4621 aacagtttat tcgaatttat tttaaattta tctatactgt ctgctcctgt caatgaatct
     4681 gtatatgcta tcctctttag atagtcctct ttcttctttt tattataaat tatatattta
     4741 atagcaatta agaataaaat acatacaaaa gaaataattt gtattgaata ttctttaaaa
     4801 atcacaccaa atggtacttt ataaggtttg tcaatagtat tagaatatat gcaatcactt
     4861 aattcttctt cagtaatttt atctattgac ttatttaaaa tagacactaa tataggatta
     4921 gttttatttg atacccctat acgcatccca taacttgtgt ctaaaacaga tataacagat
     4981 aaattctcac tctttccttt tcttaaaagt tcatcaaact gataagaact aagtaccatt
     5041 aaattagctt taccttcatt tatagcgttt aaacaacttt ctacactatt ataagatgtt
     5101 gtcttagcat tttcaaaact cttttctata taagaatcaa tagacttata gctactagtt
     5161 aaagcaatat ttaaatcatt attgagattg gtatccaatt ttcctaccat aatcattgga
     5221 atatttatgt atggatttgt caatttcata ttaaatcgct ttgccttatc attttcattg
     5281 attgaaccac atatcatagt tgcctttcca ctttttatgt aatcaaggct ctcgttataa
     5341 ttttttgttt taatatattc aaattttatg ccacattttt ttgatataat tgacattaaa
     5401 tctgaactta tgcctttaaa tgaattactt ttcttatcat aatactctac aggagaccac
     5461 tctgaatcat acaccatagt aagttttgga tttgctttaa taaaatttat ttcttgtcga
     5521 gtaaatgcta tggaattgtt aacctctctt ttgaaatatt ttttatataa ttccagctca
     5581 taatacatat catttatttt taattctttt aaagcataat ctaattcttt tgccaaatta
     5641 ggtttatctt ttgcagtagc aaaataaaaa ggttctactg aaaatttact aactattttc
     5701 attcctttag catctataat acttccacaa acaaatgcat ctgctttttt ttcattgaca
     5761 gactcaatca attcatcaat agaattacag tatatatatt caactgaaaa attattttct
     5821 tctctatact tctcaaatgc aatattatga aagtttcctc taagtacagc cactctcatt
     5881 ccattaaagg aatttatatc ttcataaaac atattattgt tactatcatc tgtatataat
     5941 gcactatatt catatcctga acttagttta ggaaaattaa atattttatt tctttcatca
     6001 tccttctgta aaggtccaag taaatctatt tcaccttttt ctaatctatc taaacattct
     6061 tcccaagacc cctttataaa ttcatactcc catcttgtat attttgagat ttctcttaga
     6121 taatcataac catatcccga ataagaatgc tcttttgatg aggatattat tccatagtca
     6181 tcacagtatc caacttttat tttttttgaa ttttcagact catcagcaaa gcatagtata
     6241 ttattaaaac atgatataat aagtaaaaat atcaaagaaa gtatcaagaa gtgcttttta
     6301 aatgctttca tatttaaaaa cctccaattt gccaacttac tttaacttat ttggttttct
     6361 agttgtaact acttttattg taatggtaaa tatatcaaat tgcaatattc tcatataata
     6421 ttcttattta actataatga atttatcatt atctatttaa ataccatgtc taataccaat
     6481 ttcttataaa tcataaaagt ataattaaca ccataattct agtgattaat tttatttcaa
     6541 aaaatgtaat aacactacca atattttaaa tatcaatagt gtcaaaaatt ttttcagatt
     6601 aattaaatct ttatatatat tttataataa tccaaatcat actaaacatt tagacagaaa
     6661 acttttttct ttaacagtaa atgcttttgt ttgaatatca gaataattct ttgaatatat
     6721 atctataaaa ccatgatttg catctaaaat ctcaatttgg atattttgtt ttctagaaag
     6781 ttggcaagaa atagaattaa catcaaatga atcgtacttt gcaaatacta acaaagtagg
     6841 acattttgga attacatcca tgtaatctct gatacgagaa ccataacaac agattatgcc
     6901 atcacaaaga gaatcttcac tacatctcca tgcaatagtt gctcccacgc tatacccagc
     6961 aataaataca tatttatact ctgattttaa atcatttata agcttttcaa cttttttata
     7021 aatatcaaaa ccaacattat ttataaaaaa atcatatgcc tcttgaactt ttgaatatgg
     7081 aaaaactctt ttttctccca gaagttcagg acaaaataca tcatatccat atttataata
     7141 cttttgacaa acatcttcta taaagctatt aatcccatag atttcatgca aaacaacaat
     7201 agctacatca cttttatgta tatactttat cagattatcc accaccttta caaaatagca
     7261 aattttttat acttagttta atatattatt ctttacatgt caattcactt ttttaaaacc
     7321 aaatttattt ttggattaat cacttgtttt taaaaattgt gtattattac tcttaaatta
     7381 ttccctattg ttaaaaacat aattgttaga aataaaaaat atagtataaa taataaaaaa
     7441 atatctcact cataacttta aaaattataa gtgagatact cttaaaatct actttatatt
     7501 aaatttattt tccaaaatat ctatttaata atcctaagaa tgctttacca tgtctagctt
     7561 cgtctttaca catttcatgt actgtatcat gtatagcatc taatcctaac tctttagctt
     7621 tcttagctaa ttttaatttt ccatcagttg caccatattc agcatcaact ctaactctta
     7681 agttttcttt tgtatctgca actacaactt ctccaagaag ttctgcaaat ttagcagcat
     7741 gctcagcttc ttcaagagct attctcttat aagcttctcc tacttctgga taaccttctc
     7801 tatcagcttg tctactcatt gctaaataca ttcccacttc tgtacactca ccaacaaagt
     7861 tagctcttaa tccttcgatt atttcttcat ctacaccttg agctactcct attctgtgct
     7921 catcagccca atttttttcg cctttcattt cttcaaattt atcagctcca actttacata
     7981 ctggacattg tgctggtgca gcatctcctt catgtatata tccacatact gtacaaacaa
     8041 atttcttcat aattaatttc ctcctaaatt ttatatatca aattaaattt tttatttatt
     8101 aagttagtct atatataccc gcaccatata cctttaaaca atttttttta aagttttttc
     8161 taagtactta ttttccctgt gtcaatagta tgtttctata ataatgtcct tttaatttta
     8221 tcaattcttt atgatttcca gattctacaa ttctaccatt ttctaaaact attatattat
     8281 cacaatttga tatagtactt aatctatgag ctataattat acttgttttt ccataagtta
     8341 aattatttat tgcctcttga attttagctt ctgttgctat atctatgttg cttgtagctt
     8401 catccaaaat aagtattgga gcatttttta ataccgctct agcaatactt attaattgtt
     8461 tttggccttc acttaaatct tcacccctat ctgataatac agtactatat ccttcacgca
     8521 ttttaactat aaatttatgt gaatttgcta ttttactagc atatataact tcttcatcag
     8581 tagcctccag atttccatac cttatatttt ccataactgt atcattgaat atataacctt
     8641 cttgtgggac tacaccaaaa cacgttctca aatagtttct gtcaatagaa taaatatcct
     8701 tactatctaa aaatatactt ccttcatcta tatgataaaa atttgtaatt agatttataa
     8761 ttgttgtttt tccacttcct gtctctccta taatggcatt tgatgtacca gctttaattt
     8821 ttaggtttat attatctaaa attttctttt ttgaatcata agaaaaactt acatttttaa
     8881 gttcaatttc tccctttaca aaatgagagt ttgaattacc atctttatta tccttaagtt
     8941 cacttttaaa catattatcc aattcttgtt cttctttttc atctataatt tcaaaaactc
     9001 tttcacatcc agctatggct gaaagtaatg tattaaatag atttgccaat tcatttaaag
     9061 gtcttgtaaa ttgtcttaca taacttaaaa aactagctat aacccctatg gttataatat
     9121 ctttagatgc aagaacacct cctataatac atatagttgc aaatcctata ttactaatca
     9181 cattcataat aggcattaaa agagaagtat aaatttgaga tttaagggaa atctttagaa
     9241 gtctatcatt gatttcttta aaatcgttta cagattttat ttcataatta aaagatttta
     9301 ctacatttat gtttgatatc atttcttcaa tatgagcatt tagttttccc aactcaactt
     9361 gttgttgttt aaaaaaaact ctagttctct ttgcaataaa attactcaca aacattactg
     9421 tcggtattaa tattacactg caaaaagtta aaattggact taatacaaac atcataataa
     9481 aagtcatcaa tatgtttaag attcctgtaa ttaattgtac tagtgaattt gatattcctg
     9541 tactgatatt atcaacatca tttgttactc tgctcataat atctccagta gaactcttat
     9601 caaaaaaggt tattggtaga cttttaaatt ttaaaaataa attactcctc atctctttaa
     9661 ctatactttg ggataatcta gctgtacata tccccttaga cagtttaact gaaaactcaa
     9721 ttatataaaa tgaaagtaaa ataattattc cttttaagaa aatggatttt gaataagtat
     9781 caatatctat tatgtcaact acctttccaa tagtgtatgg gattacagtt gatataattg
     9841 aatcaattat tactagcaaa aaaataaatc tagtaatctt cttatctttc ttaaagtaca
     9901 taaaaattct tttaaaagtt tttttaaaat ctttaggctt ttcttttttc tttaatctac
     9961 tgaccttttg atttccagta cttaaattca atatacttcc tccttaattt acatcttcac
    10021 ttactaaatt ctgcaacgta aaaatttttt tataaattgg agaactaacc agtaaatctt
    10081 catgtgtagc aaatccaacc acctctccct catccaagac taaaatctta tcacagtcaa
    10141 ttatagaact aatcttttgt gaaatagtta ttactgtaca tttcttacct tcattttctc
    10201 ttaaattgct tatatgatct tttatattct ttcttataat tgtctctgta ttaatatcca
    10261 ttgcactaaa acaatcatca agaattaata tttgtggttt ttttgctatg gctctagcta
    10321 tagtaattct ttgtctttgc cctcctgata gatttttacc cctttgaaat aaaacttcat
    10381 cataagaatt ttcgagtttt gttataaatt gatttgcatt tgctatttca catgatttta
    10441 taacatcctc tttgcgtata tttctatttc ccataactac attctccaat atactttttc
    10501 caaacacact actattttga aatactacac ctattctttc tcttaaactt tcttcagaaa
    10561 tactgtatat actatgtcct ccaatcttaa tatcgccttt atcaattata tataattttt
    10621 gaattaaatt tactaaagaa gtctttcctg aaccagtttg accaatcaca ccaatatttt
    10681 gaccttcttc tattttaaaa gatatgtttt ttaatatttt ttcacctgta tttttgttat
    10741 acgcaaaatc cacattttca aaaattatat cacaatttgc aaactttaaa tctttttctc
    10801 caatatctga acttttatct ctctcaatta cttcttcatc ctcatcaatc aaaacctcac
    10861 ttattctctc ataagacgct tttgctctaa caaaaacatt aaaaacatgt gaaatcatta
    10921 taagagatgt taatattcta gtcatatagt ttacataagc aactataatc ccaattttaa
    10981 tttctcctga ataaattaaa tatcttccaa tcagtaatat tattattatt gaaacattaa
    11041 tgattattgt aattgtaggt ctaaaaaatg acatcatagt tgtagctttt ctactatttt
    11101 taaaaagctc ataatttaac tcttcaaaac ttttgttctc ataatcgtat ctgcaaaata
    11161 gtttaactac ttttattcca cttaaaaact gtcttaattt tgaatttact ttatctgtac
    11221 tttcttgtac atttttaaat aatctatacc ctaccttcat attaagcaat attataataa
    11281 atataattaa aactgcaatt agaataatta ttgacatctt taagtttaat tttatggcta
    11341 taattaaact tcctatgcac attattggtg actttataaa aactctcatc atcccattaa
    11401 cgaattgttg catttgatta atatcattag taattctcgt tattaacgaa gctctaccaa
    11461 atttatctat ttgttttaac gagtatgtat ttatcttttc aaatacatcc tgtcttaaat
    11521 aataagaaaa atgctgagaa actcttgccg agattacact tcttattatt gcaaatattg
    11581 cacctaaaaa agttatacct atcattataa ctcctgttga tagaatgtaa tctaagttct
    11641 tatttgaaac tcctatatct attatttttg ccattatagt tggttgagct aaatcacaaa
    11701 aagcttccag tgtcaaaaaa aatactgctg ttagaaataa tactatatat ttttttatgt
    11761 attttttaaa atacctcatt caatcatccc ttcatagaat tttctcatta ccaaaagtat
    11821 atcataatgg ctaatttaat tgattatatc aattataaat atttgattta ttattaattt
    11881 ttataccata tattttataa attatttgtt
//
LOCUS       DAMJVM010000108        11708 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.112, whole genome shotgun sequence.
ACCESSION   DAMJVM010000108 DAMJVM010000000
VERSION     DAMJVM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11708
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.112"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(<1..2039)
                     /locus_tag="PC523_003001"
     CDS             complement(<1..2039)
                     /locus_tag="PC523_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HDF2476501.1"
                     /translation="MKKFIISIISLVLFFSNISLIDKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEDVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKVKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKMENSINEYPYPYAEIENNLKLDTTIVVPDNMTRGESTAVFNLASEFGKIIKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSTEEQTLLSSNCLIKQVDSPYNKN
                     KKMMVVIGSNEENLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIVLNLRYSDNLHFEKSLVTVSIN
                     DVLVGSKKLDRQHSNNDKLELKIPKDIDNKNYYQVKLVFNLNIKNSNCVTRESNNPWA
                     YVSNSSYLALSTKENEALSFESYPYPFVRNDEFNDLTVIMPDYSDSQAMTWMSKLGVT
                     LGANINSHNGNMNVIRGKEFSDKYKDTNIIVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FLSNDKLSFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKPKKEVKNSSWNMSINKTTKVFLMISFITIIVV"
     gene            complement(2062..3318)
                     /locus_tag="PC523_003002"
     CDS             complement(2062..3318)
                     /locus_tag="PC523_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2476502.1"
                     /translation="MRDLNVSECLFVFSLCSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMVSILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILED
                     IKNKYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDRNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIKNIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            complement(3367..5031)
                     /locus_tag="PC523_003003"
     CDS             complement(3367..5031)
                     /locus_tag="PC523_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2476503.1"
                     /translation="MMFKIFNKSLLKLVAILSIVLIISNFSLSSYASEITSRTDNVLI
                     VYDSKKETDYNRDILNIMRTLLGRFSSDIKLLKLSNYDGEINKNYYSHIFILGINENS
                     YDNDNKTKNLISSLNLYKGTICWLGYGIENLLEHKKYNLDYIGKTNNIVSVNYRGKSY
                     NLEEHYIFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKRFDKGRIFVRIEDVHAFRDPKDLVNIADYLSSKDIPFTIALIPAYVNPK
                     NHKITTLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLSGLRVCIENGIYPLAFEAPHYAMESDGYKELKKYFSTYMGQHQNNN
                     KKFSTNTYPYIIRDTEEFNILIPENLGYIDPEDKFTFQHIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDRDLEE
                     NIKSDIRFKSISNISKILIFIVSISVLIFMIIFIYFKRIDKKKFLK"
     gene            complement(5021..5869)
                     /locus_tag="PC523_003004"
     CDS             complement(5021..5869)
                     /locus_tag="PC523_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2476504.1"
                     /translation="MNIVNKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSIVGLITSSIIIFMYTSYILYNNITYNMDVEFISYMWIIAIPISSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGESKTNKIVKYIGSNIIECTRNEDIIYSLQKDMLGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYRDSFGDSINFKNIVEE
                     ELQYDV"
     gene            complement(6128..7180)
                     /locus_tag="PC523_003005"
     CDS             complement(6128..7180)
                     /locus_tag="PC523_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2476505.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPIDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITENPVAYPSKEVLNKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            complement(7180..7854)
                     /locus_tag="PC523_003006"
     CDS             complement(7180..7854)
                     /locus_tag="PC523_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2476506.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGTK"
     gene            complement(7959..8795)
                     /locus_tag="PC523_003007"
     CDS             complement(7959..8795)
                     /locus_tag="PC523_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2476507.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYIKVFGRSILLAGGATLICLVVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            complement(8797..9840)
                     /locus_tag="PC523_003008"
     CDS             complement(8797..9840)
                     /locus_tag="PC523_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2476508.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKESN"
     gene            complement(9859..10398)
                     /locus_tag="PC523_003009"
     CDS             complement(9859..10398)
                     /locus_tag="PC523_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2476509.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            complement(10834..11523)
                     /locus_tag="PC523_003010"
     CDS             complement(10834..11523)
                     /locus_tag="PC523_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476510.1"
                     /translation="MLQKVLWKFLKLVFGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLILLAVGMVFYMGSGLGSGPRDGLMVALQKKTGKSINLIRGIIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAATQNIVK"
BASE COUNT         3895 a         1999 c         1140 g         4674 t
ORIGIN      
        1 atgactacaa ttatagtaat aaaagaaatc attaaaaata ctttggttgt cttatttata
       61 ctcatattcc aacttgaatt ttttacttct ttcttaggtt ttaaattata agctaaatct
      121 tctacctctc catattcatc aattatcaat gtatctcctt ttaaatcatt aactttactt
      181 ttattcaaca aataatccat tcctaaatat aagttttttt cattcattga actaattacc
      241 atgatatttt tttgattatt atatggtgat tttattaatt gaatggtaga aatatttttc
      301 ccataatcat caataaagct taacttatca tttgataaaa aattggagta atttttatcg
      361 aattttatat ttaaattatt atttaacatc tttataacag aattattatg cggtactcca
      421 aaaactataa tattggtgtc cttatacttg tcgctaaact cttttcctct aattacattc
      481 atatttccat tatgtgaatt tatatttgca cccaaagtaa cacctagttt agacatccat
      541 gtcatggctt gtgaatctga ataatctggc attatcacag ttaaatcatt aaactcatca
      601 tttctaacaa atggatacgg ataactttca aaagataaag cttcattttc ttttgtagat
      661 aacgccaaat aagaactatt tgatacatat gcccaaggat tattactctc tctagtaaca
      721 caattcgaat tttttatatt caaattaaat accaatttga cttgataata atttttatta
      781 tctatatctt taggtatttt taattctaac ttatcattat tagaatgttg cctatccaat
      841 tttttacttc ccacaagaac atcatttata gacactgtaa ctaaagattt ttcaaaatgt
      901 aaattgtcag agtatcttaa attcaagaca atctttgaac catcatctag taccttacct
      961 gtcggtattt tgacatcaaa caaagcttgt tgattaaatg ctccttccaa caaaaagtca
     1021 gaatatccta agtcttttaa agttaaatga cctaagttta gtttttgttc tctattgatt
     1081 tttatattag tctctttgtt tacaagtact gatgatgata aaacttgatt agaaagtcta
     1141 ttttctatta ataaatttga tgcctttatt aaattttctt cattactacc aattacaacc
     1201 atcattttct tattcttatt gtatggtgaa tctacttgct tgattagaca gttcgaagat
     1261 aatagtgttt gttcctctgt acttaaaatg tccaagattt cttctgctgt attttcaggt
     1321 ttaccaatat atataatatt atcatctgac caatttttca tttctgaata taatttaaca
     1381 tctaacttta aatcatcatt ttttattatt tttccaaatt ctgatgccaa attaaataca
     1441 gctgtagact ctcctctagt catattatct ggtactacta ttgttgtatc taatttaaga
     1501 ttattttcaa tttctgcata tggatacgga tattcattta ttgaattttc catctttttt
     1561 tgtttatatc taattgatat atatgattct ttgtgaataa ccatccagtt cccagtattg
     1621 gaatcatctt ggcaaatttt atcagatatt gttttatatg tttttacctt tatttcgtta
     1681 tagccttgaa ttatataatc ttttggaact aatacttcta gtgtattttt ataatttgtc
     1741 ttagcattta atttaataga ttttattggt acattattta tcaaaacagt taaacttgaa
     1801 acatctccat ttaatatttg gctcttacta aaattaagat gtaataaaac gtcttcaata
     1861 tcccaatttt tatttacttc aaaaaaagta ctattacttc ctataacacc atcaatagtt
     1921 atatctcttt caaacttata atttttaact ttagtctcgt cagcatttac tttgtctata
     1981 agtgatatat ttgaaaaaaa caatactaaa gatataattg atataataaa ttttttcatg
     2041 ttaattcctc ctgtacatga attaaaatct ttctgtttta taccatttaa cttctctttt
     2101 aaaaattata tcttttagat actctatcat tccttttata gcgacaaaca accatagttg
     2161 agagtatgta aaatacatta ttgcaactat gaatatattt ttaattgttg cttcaccttt
     2221 ttctatagta aggcttatac tcacttctat aataaataaa agataagata gtatccatat
     2281 tagaaaaaag ttaattggaa tagaaatttc tactagctta gatatactta aaacaaataa
     2341 aatatcggat ataatgacag atgttaaaaa tagaaaatat acagaaaaaa agtaagctat
     2401 atcaaataca atcttatttc ttccttgctt aaatatattt tttatgtatt tcattaatac
     2461 atatatattc ccttttgccc atctagtacg ttgttttatc cataccttaa cagtttctgg
     2521 ctcttgttcc catgttatag attgtggtac aagctttatc ttgtatccca atttatatat
     2581 cctaaaactt atttctgtat cttctgcaat tgctttacta tcccatcctc caatttcctc
     2641 tatgatactt ctcctcaata taaagttagt tcctggtatt gtacatagat taaaaagttg
     2701 ccatctacca gcttgtgaca tccattgaaa acttaatgtt tctatgttta taaatttggt
     2761 taataggttt ttatttttat ttcgagtcct aaatttccca ataacagcac ctaattcatc
     2821 attcattact atagtttgta ctaaatatct aagtgcattt ctatctggtg tgttatctgc
     2881 atcataaaca gctataaaat ctccttttga aatcgtatat ccaatattta atgcattaga
     2941 ttttcctttt ccacctgtta aactatctgt atttattatg gtgaaattat agttattgta
     3001 cttattcttt atatcttcta aaatttcttt actattatca gaagaattgt catttataac
     3061 aataagttcc attttactct taggataatt gagtaataat aaagattcca cagttctacc
     3121 tatcacttta gcttcattat gagcaggaac aagtattgat accataggat attcatcaat
     3181 ttcttttatt tctttatcaa agtttttaaa gtaaaatata tatccaccca tagctaaaat
     3241 aatatttata agcaataaag accatatact acacagacta aatacaaaca gacactctga
     3301 tacatttaaa tctctcataa atttctccta aaatatttta aaatattaat tctaatataa
     3361 aatcatttat tttaaaaatt tctttttatc tattctctta aaatatataa atataatcat
     3421 aaatattaaa acacttatag atactatgaa tattaaaatt ttactaatat tagatataga
     3481 cttaaatcta atatcacttt taatattttc ttctaaatcc ctatcataat tgactataat
     3541 ctcatctcca ttatttctta tttgtatttc atcaactttt acccaattat taaaatctct
     3601 taaattcata aattctatat tctgtttttc taaatattct atcgtgtttt ttagatactc
     3661 tatgttcaaa tatgaatgga agaaaaaacc tccactaaaa cctcttacaa tagataattt
     3721 atctaaattt tcttttatat gttgaaaagt aaacttatct tctgggtcta tatatcctaa
     3781 attttcaggt ataagtatat taaactcttc tgtatcccta ataatataag gatatgtatt
     3841 tgtactaaat tttttattgt tattttgatg ttgccccata taagtagaaa aatatttttt
     3901 tagttcttta tatccatctg attccattgc ataatgtggc gcttcaaatg caagaggata
     3961 aattccattc tctatacata ctctaagacc acttaaaact ctatctttga caaatatctt
     4021 catattttct ttcaaaggct catctttttt accatcccaa aattcatatc cttctccaga
     4081 aacttcttct ttcttatatt gatgagtata tccatgcaat attacagttc cacctttatc
     4141 ttgcatatat tttattgctt tgacaatttc agggctttct gataaagttg ttattttatg
     4201 attttttgga tttacataag ctggtatcaa tgcaattgta aatggtatat ccttactact
     4261 taaataatca gctatattta caaggtcttt tgggtctctg aaagcatgta catcttctat
     4321 tcttacaaat atacgtcctt tatcaaatct cttaatatta aaaatatcgt taagtgaatc
     4381 gcaaaatata taaaataaaa caccatctaa atctaatttc gatacataaa aaaggttttt
     4441 atcatttatt atatatggat acttatttaa ggtatcatta atacttccta ttactttatt
     4501 acttgtatct tttgattcta caatgttaaa aatataatgt tcttctaaat tatatgattt
     4561 acccctataa tttacactaa ctatattatt tgtttttcct atatagtcta aattgtattt
     4621 tttatgttcc aaaagattct ctataccata tcctagccaa catatagttc ctttatataa
     4681 atttaaagaa ctaatcaggt ttttagtttt attatcatta tcatatgaat tttcattgat
     4741 tcctaaaata aatatgtgac tgtaataatt tttatttatc tcaccatcat agttactcaa
     4801 ttttaatagc tttatatccg aactaaatct tcccaataga gtcctcataa tatttaaaat
     4861 atctctatta taatctgttt ctttcttaga atcatatacg attaatacat tatctgttct
     4921 agaagtaatt tctgatgcat atgaagatag actaaaattt gatattatca gtactattga
     4981 taatatagct actaacttta acagactttt attgaatatt ttaaacatca tattgaagtt
     5041 cctcttcaac tatattctta aaatttatac tatctccaaa actatcccta tattgtaaaa
     5101 atgcaatttt gacatctata tttacatatt ttcccctatt gtttaaatct aaatttagtt
     5161 cttttatcct tttctttatt ctgtctttaa caacttttga accttccaaa gatgtatttg
     5221 gcattagtat ccctagcata tctttttgta aactatatat tatatcctca ttccttgtac
     5281 attctataat atttgaacca atatatttta ctattttatt agttttgctt tcaccaaata
     5341 tagtctgtag gtttccatag tatggtaatt ttactatcat aagagaaaaa tcagtattat
     5401 gtctaataga tttacttatc tccatattta catcattata aaaaatcttt aaatttctaa
     5461 gtccagtttc actatcaata gtcactaatt ccttatactg ttcagatagt tttttatttg
     5521 tgttttgcaa ttcatttatg cttttgttca gattacccat tataattgaa gatattggta
     5581 ttgctattat ccacatatat gatataaatt caacatccat attgtaagta atattgttat
     5641 ataaaatata ggatgtatac ataaatataa ttattgaact tgttattaat cccacaattg
     5701 agtttgacaa ataagttaac attattataa aaaaaattat tgctagcatt ataaacacat
     5761 tcaatttatt ttctgtccta atggatagta ttactattcc aacaattaaa aatataaaaa
     5821 cgagcaaagc caatattaat aaatctattt ttttatttac aatattcatt ttttataaat
     5881 ttacacctcc aattattcta ttaagttgcc cattctttct aattattatt aaagtcatta
     5941 tacaaataag ttttcattta ttaacaaagt atttaaattg tgtatgaata taacttaata
     6001 aaaaaatact gtctcaaagt aaatttttta actcataaat cactcttttt tgtacgaaat
     6061 cgaatatgtt ttcaacaata aaaaagaggc ttatctcaat ttcgagacaa cctctttata
     6121 gttattatta ttttgatttt aactctatcc aagccttatc atataatttt atattttcac
     6181 caatatcttc aaaagtttca catttattaa gtacttcttt agatggatat gcaacaggat
     6241 tctcagttat ttccgggtct aataatttta gagctcctgt attaggtgtt gaatacccta
     6301 tgtactcagc atttactttt gcattctctg ggtccaataa aaagtttata aacaactcag
     6361 cttctttctt attttgtgct gttgtaggta cacacatagc atctacccat ttattagttc
     6421 cctctttagg tatagcataa tcaaggtcag gattttgttc cattagtgta actgcatccc
     6481 cagaatacag catacccatt gccacttctc caccaagcaa tctatccttg ccttcatcat
     6541 ttacataagc caatactaaa tccttttgtt tcattaaaag atttttagct tcatttattt
     6601 cctttggatt tacactgttc atgctatatc ctaatttttt aagagttatt cctatagtat
     6661 ctctaacaga gtcaaacatc attatatttc ccttatactt tggattccaa agtatattcc
     6721 agctatcaat agggtcttta acttctttct tattgtacaa tattccaaga gttccccaca
     6781 tatatgcaac actatattca ttagttttgt cgtatacagg atttaaaaaa ctcttatcta
     6841 aatacttcat attaggtata ttcttaaaat ctaatttttc caaaagacct tcatttttca
     6901 ttttctctac catatagtct gaaggaaata ctaaatcata gtgtgtacta ccacttttta
     6961 ccttttgata catcatttca ttagtatcat aagttgaata ttgaacatct attcctgttt
     7021 ctttttcaaa cttatctata agagactcat ctatataatc tccaacatta tatacattta
     7081 aaacttttgt agaatctgtt gctttattac atcctgtaaa taaagtaact gtcatcatac
     7141 ctacagctat aagggatatt gcttttttaa acttaatcat tatttagtcc cccttactat
     7201 agattctttt ttattagcca gtaaaagtaa ccctagaaca actgcaaaca ttattgttga
     7261 taatgcattt atttcaggtt ttatacctct tctagccatt ccatagattt ctatagaaag
     7321 attgcttaca ccatttccag cattaaagaa acttattata aagtcatcta ttgacatagt
     7381 aaacgcaatt aaaaatccag aaactatacc tggttttatt tgtggtaaaa ttacttttct
     7441 aagagcgtat ataggtgttg ctcctaaatc catagctgca tcctctatat tatcaggtaa
     7501 ttgcttcatt tttggcaaaa ctgaaagtat aacgtatggc aaacaaaaca ttatatgtgc
     7561 taataacatt gttgtaaatc caaattccat ttttacaaat acaaatagac tcataagagc
     7621 tactgctgta actatctctg tattcaaaat aggaagatag tttacattta gtattaattt
     7681 cttactctta cctctcatct tatgtattcc tatagcactt atagtcccta ctattgtaga
     7741 tattacagac gccaatatag ctattacaat agtataatat aaagcactca ttatactctc
     7801 attttgaagt aattgaccat accatttcca tgtaaatcca ttccatcttg ccattgattt
     7861 tgaatcatta aaagaaaata gtaccaaagc aaatatgggt gcatacaaaa ataagaatac
     7921 taatgctaaa tatatattag aagttatttt ctttaatttt aaaatagtaa cccaccgcct
     7981 tctttgtcag attcatctcc aaattttgac attatagaca tagatattaa tattactatc
     8041 atcataaata ttgatatagc tgaaccaaag ttccaatctc caacgactgt aaactgttgc
     8101 tcaatcaagt ctccaaccag catgatttta cctccaccta acagtcttga tattgcaaaa
     8161 gtagtgactg ccggcataaa taccatagtt attccagaca ttacacctgg caaactcaaa
     8221 ggaaatataa tttttctaaa cactgtcata ttatttgcac ccaagtcatg agcagcgttt
     8281 atcaaatcat tatccatctt tgacagtgct gtatatattg gaagaaccat aaatggtaaa
     8341 aagttataca ccatacccaa aagtatagca aagttagtat acaaaattgc taatggttgt
     8401 attccaaacc aacctaaaaa tgtatttaaa agtccatttt ttcctaatat agcaacccat
     8461 gcatatgttc ttaaaagaaa gttcatccac ataggaagaa taaacaataa tattaatgaa
     8521 cctcttctgc ttacacgagc ctttgaaatt atatatgcca caggataacc aactacaaga
     8581 catatcaatg tcgctcctcc tgctaaaaga atagaacgcc caaaaacttt tatatatata
     8641 gggtcgatta cttctttata gttctcaagt gtaaaagcat aacctccacc tgattctttt
     8701 gtaaaactaa aaaataccac tagtattaaa ggaattatta caaatatcgc actccaaact
     8761 acatatggat atgctaaaaa agattttctt ttcatcttag ttactctctt ttctcattat
     8821 atggatatct tctggatata tatccatgcc tagttcagtc ccaacctcag catttttagt
     8881 attatgaagt atccacaatc ttccattttc attcaattct atctcgtaat gcactccttt
     8941 aaacactgta gatgtaactt tcccctttaa cattccttct tctggcttta ccatttttat
     9001 atcttctggt cttattacta cctctatatt ttcgtttttt tcgaatcctt tatcaacaca
     9061 ctcaaaatcc ctattacaga aattaacttt ataatcctct atcataattc catcaaatat
     9121 gttactttca cctataaact tagctacaaa tgaattttta ggttcattgt atatatcttg
     9181 tggtgttccc atttgttgaa tttttccctt atacattaca acaatagtat ctgacatagt
     9241 aagagcctct tcttggtcat gagttacaaa cacaaatgtt attcctaatt ttttttggat
     9301 tctctttaat tctatttgca tctcttgtct taattttaaa tcaagtgctc caagtggttc
     9361 atctagtaac aatacttttg gttcattgac tagagctctt gctattgcaa ttctctgttg
     9421 ttgtcctcca ctcaatgatt caacttgtcg tctttcaaaa ccttctagtg caacaagttt
     9481 caaaacctct tttactttag catctattac atcttttgga gtctttttaa tttttagtcc
     9541 aaaagctacg ttttcataaa catccatgtg tggaaataaa gcatattttt gaaatactgt
     9601 gtttacttgt ctttcatatg gtggcaaatt atttatctcc ttaccttcaa ataaaacttt
     9661 accatcatca gcatactcaa accctgctat tatttttaat gttgtagttt ttccacatcc
     9721 acttggtcca agtaatgtta aaaactcatt tttctttata tctaaattga aattatctaa
     9781 aacagtaagt tcctcataat gtttagatat atcccttaat tctattatat tttctaccaa
     9841 ttattaaaca cctctattct aaaatgatgg tggcgtactt acccaaatca cttttgctgt
     9901 agttttgcct tcgtttgata tatagtgatt tgctttcggt ctaaagtaaa aactctctcc
     9961 tttttttact ttatttttct tttcgcctat atggacgtat attgaacctg ctaatacata
    10021 tccaaattct tcaccttcgt gtggcttttc ttctttatac tgtcctcctg gatacaatgt
    10081 tattattatt ggctccattt catttttttg tgaatttgga attaaccact ttaatttata
    10141 ttttaagtct tcatcttcag tttcaaacat atcttctttt gtaaaactga ttctttcatc
    10201 atctatttca ttaaaaaatt cccttaaatt tgtacctagt atttcaagta tatctataag
    10261 ggtagctatg gatggtgatg ttaagttatt ttctaattga gaaataaatc cctttgacaa
    10321 ttcacatcta ttagctaact cttcctgggt cagttgtttt tctgttctta atctttttat
    10381 cttttcacct atatccatgt tgcacctcct agtttctgtt ttattttact aaacattttg
    10441 tttactaaac attgagttta aaatttctaa acaacatagg taattatata taaaatttgg
    10501 atataataca agagtttttt tgacattttt ttattttttt atttagaata aatacaagca
    10561 ttttaaaggc tttggaaaat acacagttta aaggtgttta tattaatatc tataaagttt
    10621 agtatctcta attataaatt aattcattca ttaaatagag aataagactt ctaatactaa
    10681 aaaaacattg aacaaataaa aaattgctag aaatctatat tttatagtgt tctagcaatc
    10741 cttatatttt aatattcttt taatatttag tttatatctc taaacaaaat tttaatttaa
    10801 aatacctaac atttcgttga atttattttt aaattactta actatatttt gagtagcagc
    10861 ttttttctct ccactcatat acactttgat aaattttata tcatctacta taaatctatg
    10921 tttaatctta ctacaatcta atttaaataa gccaaatacc atttgtgtaa aataccccaa
    10981 tccaaatgca cttattatag ttcctatacc aacttttcca cctaataaaa atcctactat
    11041 taatgctcca acttctataa tacctcttat taagttaatt gactttcctg ttttcttttg
    11101 aagtgcaacc atcaaaccat ctctaggtcc acttcctaaa ccacttccca tataaaagac
    11161 cattccaaca gccaaaagaa ttaatcctaa tatcaacata attattccta caaataaact
    11221 atcagcctct ggaattaaat tgctatataa aagtaaatcc ataaaaaatc caattagtag
    11281 catgttaaaa acagttcccc atcctacatt ttctccaaga acgacatcag caataactac
    11341 tatagaaccc acaattatag aggcagttcc tatagttatt cctatatgat ttgaaagtcc
    11401 ttgatgaaat acatcccagg gagataagcc aaggttacaa tttatcgtca atactatacc
    11461 aactgaacat ataaataaac caaacactag ctttaaaaat ttccataata ctttttgcaa
    11521 caaattctac ccctcctaaa cataatttaa ctactcactc taaatatcga aagccagcag
    11581 ttttctcttg aactactcca cattaactat cttatgtgat atttaaatct ggagatttct
    11641 attaaataat acacttctat gatacatcat tttatgctaa ataactacca ttttattaca
    11701 atttagtt
//
LOCUS       DAMJVM010000109        11484 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.113, whole genome shotgun sequence.
ACCESSION   DAMJVM010000109 DAMJVM010000000
VERSION     DAMJVM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.113"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            21..797
                     /locus_tag="PC523_003011"
     CDS             21..797
                     /locus_tag="PC523_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2476511.1"
                     /translation="MEYIIFDLEFNQGFDKKFNKTISNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKEIHPFISRMTNIKDSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFILEDAKQRKNK"
     gene            complement(889..1641)
                     /gene="xth"
                     /locus_tag="PC523_003012"
     CDS             complement(889..1641)
                     /gene="xth"
                     /locus_tag="PC523_003012"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDF2476512.1"
                     /translation="MRFISWNVNGIRACAGKGFLDFFKEVDADVFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLISADIHTEVLGSDHCPVELEIK"
     gene            complement(1762..2712)
                     /gene="galT"
                     /locus_tag="PC523_003013"
     CDS             complement(1762..2712)
                     /gene="galT"
                     /locus_tag="PC523_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDF2476513.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDKEC
                     PFCKGNEAYATDALFEIEGEEGWLVKSVYNKFPIIDDMARDIYGVHEVMIESSKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLNRRVVYNGEAFLVIIPYATKYSGE
                     VRIIFKDKIKFGELNDNNIKELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEELTKKLKID"
     gene            3097..4293
                     /locus_tag="PC523_003014"
     CDS             3097..4293
                     /locus_tag="PC523_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2476514.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYNMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(4512..4964)
                     /locus_tag="PC523_003015"
     CDS             complement(4512..4964)
                     /locus_tag="PC523_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2476515.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(5265..6710)
                     /locus_tag="PC523_003016"
     CDS             complement(5265..6710)
                     /locus_tag="PC523_003016"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDF2476516.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYNSSYAVSGQTYTRKLDYQVVSILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINKRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            complement(6849..7760)
                     /locus_tag="PC523_003017"
     CDS             complement(6849..7760)
                     /locus_tag="PC523_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2476517.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKKY"
     gene            8017..8841
                     /locus_tag="PC523_003018"
     CDS             8017..8841
                     /locus_tag="PC523_003018"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDF2476518.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKRPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            8881..9411
                     /gene="lepB"
                     /locus_tag="PC523_003019"
     CDS             8881..9411
                     /gene="lepB"
                     /locus_tag="PC523_003019"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2476519.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRIIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKIFAMGDNRENSNDSRFPDVGMIDEDEILG
                     KVMVRLLPLDNIGKVD"
     gene            complement(9491..11272)
                     /locus_tag="PC523_003020"
     CDS             complement(9491..11272)
                     /locus_tag="PC523_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDF2476520.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINKDVKVVPKVTPFKGIN
                     TDLLYVKDGKILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLDEYLNDENDEINLNLFLLDVC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEEVQIKDVISIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLIIFTHTKQLRNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKAFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
BASE COUNT         3897 a         1708 c         1376 g         4503 t
ORIGIN      
        1 aaatttagga gataaacttt atggaataca taattttcga tttagaattt aatcaaggat
       61 ttgataaaaa attcaataaa actatatcca atgaaaagtg tccttttgaa attatacaaa
      121 taggagcaat taaattggat tctaaattca atataataga tacatttaat agctatgtaa
      181 agcctacgat ttataaagaa atacatcctt ttattagtcg tatgactaat attaaagatt
      241 ctgattttaa tgattcgcct actttccctg aagtttacaa caattttata aaatttatat
      301 catcacaaga ccctatatta tgtgtctggg gagctggaga tttaaaggaa ttatacagga
      361 atatcaacta ccacaaacta ccatcaaatt ccttacctaa atcttatatt aatatacagc
      421 aacatgcatc taaatatttt aataatccag caggaaaaag cattggatta caaaatgcta
      481 tttctatatt ggaacttgat gaaaaaatgt cttatcacaa tgctttaaat gatgcatatt
      541 atactgctaa agtttttatt aagatataca atccaagtat agtaccagat atatatcttt
      601 atacaagtat caaacctaaa actataagat attccaataa aaaaagagtt gattatgata
      661 agttatttga tgagtttagg aaaattttga atcgtgaatt gactaaagat gagaaaaaaa
      721 tcattaactt agcttataac atgggaaaaa caaatcaatt tatattagaa gatgctaaac
      781 aaagaaaaaa caaataaagt ttacttaggt taaaaatagg catgtagatt aaataattag
      841 ttagctttaa atttatatct acagcccaat ttttatccta attaatcttt attttatttc
      901 taactcaact ggacaatggt ctgaacctaa tacttcagtg tgtatatctg cacttatcaa
      961 tctatcttct aattttttgg atgcacaaaa ataatctatt ctccatcctg cattattttt
     1021 tcttgcatta aatctatatg accaccaact atagacacct tctttatcag gattaaaata
     1081 tctatatgtg tctataaacc cactatctaa taatttagaa aacttctctc tttcttcttt
     1141 agtgaaacct gcatttttca tattattttt aggatttttt aaatctattt ctttatgtgc
     1201 aacgttcata tctccacaca caattacagg cttatgatta tctagttttg ttaaatagtc
     1261 tataaagtcg tcttcccatc tagttctata ttctagtctt tttaattcag attgagaatt
     1321 tggtgtatac actgtaacaa agtaaaaatc ttcaaactct agagttatca ctctaccctc
     1381 tttgtcatgt tcttctatat ttattccata cattactttt aaaggctctt tttttgtaaa
     1441 tatagcagta cctgaataac cctttctttc agcataattc caatattgaa aatatcctgg
     1501 caaatccaac tctatttgtc cttcttgtaa tttagtttct tgtaagcaaa atacatctgc
     1561 atcgacttct ttgaaaaagt ccaaaaatcc tttcccagca caagctctta ttccattaac
     1621 attccatgat ataaatctca tactatactc ctcatctaaa cagtaaattt cttgttattc
     1681 ttcatttgct tttattttaa caataatata ctataatttc atctaaaatt actatttact
     1741 taatacctaa aaatatatgt tttaatcaat tttcaacttt tttgttagct cttcaggact
     1801 cattgaagaa acatacatgt ttgttccaag ttcaaatcct ccaaaattgt attttcttgg
     1861 gattatgtgc atatgaacat taaaatacga ttttgtttca atctttgttg gatgagtgtg
     1921 aatacataag ttaaatggca tatatccatt gatattgtac aatttcttaa acaacttttc
     1981 aaatattgta gaaagttcct ttatattatt atcatttaac tcaccaaact ttattttatc
     2041 tttaaatatt attcttactt ctccactata cttggttgca tatggtataa tcactaaaaa
     2101 agcttctcca ttataaacaa ctcttctatt taaacttatt tcattttcta ttaaatcatc
     2161 atacaaagag cttttattgc tgtcataata ctcctttgaa actaacagtt cattcgtaat
     2221 ttcaggagga ataaaagaca tagacaatat ttgagcatgt ggatgcatca aagatgctcc
     2281 tgcctttctt aagaagtttt tgaagatact aacatactcc actttatcat ctttggacaa
     2341 atctctaaat ctatttctat acataaaaaa aacatcttca aattcttttt gactcatgtt
     2401 ataaaaacta cgattatgtt tactgctctc tatcattact tcatgaacac catatatgtc
     2461 tcttgccata tcatcaatta tagggaactt attgtagaca gattttacaa gccatccttc
     2521 ttcaccttct atttcaaata atgcatcagt tgcataagct tcatttcctt tgcaaaatgg
     2581 acattcttta tcgtattcat tagtttcttc atcttcattt ggtatatctg ctgtatctaa
     2641 aggtcttttt aacctatcag tagcaaatat aacaacatca ttggtaattg gatctattct
     2701 taattctttc atagttaaca cctcttagtt aatagccttt aatataatta tatgatattt
     2761 atcgtatctg ttaaataaat ttataatatt ttttataatt ttgcatattt attattatat
     2821 attttgtcta tagctgaaag attataacta aaaaattact atcagtgtat cagaatatta
     2881 tcttaccata aattaattca taaatacata atttagtggc ataaacatgg gtatttaact
     2941 ataatttgtt aaaagtttat tttaaaacta tattaatttt ttttgatgat ttttacccat
     3001 aagtatgtta ttattatctt gtaaagatgt cttgtattca aacatacaat ttacataata
     3061 caatatacgt atttaaatta aatagggggt attaaaatga attattcaaa cagagttagt
     3121 gcaatgcaag cctctccaat cagaaaactt gttccatttg cacaagcagc aaaagataaa
     3181 ggaatcaaag tatatcactt aaacatagga caaccagaca taaaaactcc taagggattt
     3241 tttgatgctg ttaaaaactt tgatagcgaa gtcttagaat atgctacatc agaaggtatt
     3301 ccagaattat tagaagcact tcaaaattac tataaaactt acaatatgaa ttttgaaaaa
     3361 gatgaattac tagtaactaa tggaggtagt gaagctttac ttttcactat gatggcagtt
     3421 tgtgacccag gagataatct attagttcca gagccattct acacaaacta caatggattt
     3481 ggtcaatctg taaacgtaga agttaatgct gttacaacaa aagcagaaaa cggtttccac
     3541 cttccatcta aagaagaaat actgtctaag gtagatgata aaactaaggc tataattctt
     3601 tctaacccag gaaatccaac tggtgcaatt tatacaaaag aagaacttaa tatcctagct
     3661 gaaatagcta aagaaaaaga tttatggata atagcagacg aagtttatag agagtttgtt
     3721 tatgatgggc ttgagtatac aagctgtggt aacttagagg gtgtacagga tagagttata
     3781 ataatagaca gtgtttctaa gagatacagt gcttgtggtg ctagaattgg ttctatcgct
     3841 tgtaaaaata aaggtcttat agctcaaata cttaaattat gccaaggaag actttgtgtt
     3901 cctacattag agcaaatagg agctgttgaa ttatataaaa ctcctgtttc ttacttcaaa
     3961 gaagttaatg aagagtacaa aaaaagaaga gatgtattat acaatgaatt aatgaaggtt
     4021 gaaggagtta tatgtaaaaa accaactggt gctttctata tagttgctaa attaccagtt
     4081 gaaaatgcag aagacttcac aatttggatg ttaaaagaat ttaacaagga taatgaaaca
     4141 gttatggttt gccctgctga aggattctat gctactccag gtttaggacg tgatgaaata
     4201 agattagctt acatcctaaa tgaaaaagac cttcatagag ctgctacatt attaaaagaa
     4261 ggtttagagc aatatgtagc tttacaaaaa taatttttta aaattaattt atataaattt
     4321 aatgataaaa aatatcccct tttcagatat tatttgaaaa ggggatattt tttatagaga
     4381 ttataaaaaa gcacatcaag aaaatttaag atatactttt tagattgtat ttagatttat
     4441 aattatatag ctaaattcat aacaaaaagt taatcttaat actattaaat ataaaaattt
     4501 ttgtattttt tctatctaaa ttcttctttg ttcaatatat attgaagagt atttattgct
     4561 tctaaatatt tttttctgtc ttcttcagtt aatttagaaa gtttttctcc taaaccatca
     4621 cttaacttat ccataaaatc agttgctatc ttatctccct cttcagtaat atcaattaga
     4681 tattttcttc tatcaactaa atctcttttt cttgttacat acttcttatc agttaaatca
     4741 tcaaccatac tagttaaact gcctttttct atatttaact tagagcataa atcagtcata
     4801 cttattactc cataattttt tatgaaaact aatgctctta attgtgtttt atttacatta
     4861 taatctggtg caaatttttt taggtcttct aaatacaggc tagaataaat cttaggaaat
     4921 gtttttacta aaaaacttat ggtatctttt actatatagt tcatataatc acctcggttg
     4981 ttttaaaatt tattaaatta tctaaataac tttatttcag tttacattaa aaatattgca
     5041 agtacaatag tttttgctga atgtttaaat ttaattagta atcttatcat tttttatttt
     5101 tatttatatt aaatactatt tatatagcta ttttattaaa ttcatggtat agtaagtaaa
     5161 gaagaactct tcacagcggt gaagagaaga cgctagccca ttaaaacggc tatcttattt
     5221 ataaataaga tagccgtttt taattatatt ttatctgtat aattctatac ccttaagtct
     5281 acatctactt ttcctaagtc ttctttataa tcttttatag caggttctac aacttcattt
     5341 acaaactcta caacttgttc tggagctcta cctataaagt tttttgggtc cattatagat
     5401 aatatttctt ctttgctcat cttaaatgaa tcatcattca ttataagttc aatcaaatta
     5461 ttatccttac cttcatgttt tactctatag gcagctttca tagaatattc tcttattatt
     5521 tcatgtaatt cctgtctatc tccacctctt tttacagctt ccataagtat tgtttctgta
     5581 gccataaaag gaagttcttc atttacacgt ttatttatca tattttcata tactactaaa
     5641 ccatcagtta catttatccc tatttcaagt atagcatctg ctgccataaa tgcttgaggt
     5701 attgccaatc tcttattagc tgagtcatct aatgttctct ctaaccattg agtagcttgt
     5761 accattgcag ggcttataga ctcagagatt atatacttag ataaagatgc tattctctca
     5821 cttctcattg ggtttctctt ataagccatt gctgatgaac ctatttgatt tttttcaaat
     5881 ggctcttcta attcttttaa actttgtaaa agtcttatat cattagtcat tttatgcata
     5941 ctttgagcta tgcctgataa tatgcttact acttgataat ctaactttct agtatatgtc
     6001 tgtccactta ctgcataaga agaattgtat cccatctttt tgcaaatttt cttatctaac
     6061 tcttttactt tttcatggtc tccttcaaat aagcttaaaa aacttgcttg agtaccagta
     6121 gtacctttaa ctcctcttaa cttcatatta tctattctat agtttaaatc ttccaaatca
     6181 agaattaaat cttgagccca taaacaagct ctttttccta ctgttgtaag ttgagcagct
     6241 tggaagtgtg taaatccaag tgctggcata tctttgtact taagtgcaaa ttcttttaaa
     6301 ttgtttatta aatttacaag ctttactctt agaagtttta aagcttcatt catttgtata
     6361 acatctgtat tgtctccaac atatgcagac gttgccccta aatgtattat tcctttggct
     6421 ttttcacatt gtaacccata agcttttaca tgagacatta catcatgtct tacttctttt
     6481 tctatttttc tagcttcatc aaaatttata ttatctatat tagctcttag ctcatctaat
     6541 tgttcttcag ttatattgat tcctagctca tgttcacctt ctgcaagtgc aacccataac
     6601 ctcctccatg tagagaattt aaattgtgga gaaaatatat aactcatgtc cttgctacaa
     6661 tatctatctg taagtgggtt tgaataagta gtatatttat tatctatcat tatattagca
     6721 ccttccttaa atcttttgtc gtatgattac tgtgtacaca ttagtacata aataattata
     6781 tataatttta cgaactataa caagactttt ttattttttg ttcatattta tatagtgttt
     6841 tttatttatt aatatttttt atttatatcc aaattatcct tttcaattaa gtaatccact
     6901 atttcactat caacaaaatt taatacatac ttatatctaa ttgcatagta tttatcactc
     6961 catccaagct ggttttttac atcgctctca tctgccccaa gccttaaact tattgctgca
     7021 aatgaatgtc taaaatctct tgctgaatat tgagaaagcc cagctaaatc taatgctttt
     7081 ttgactataa gtctgacatt tctatctgtt atagaattac tcttttgagt tgtaaaaata
     7141 aaatcatcac taggtattat tacctctgga agcccagaat aattcctgta atcttctaaa
     7201 agtttaaaaa agtatggatg taatttaact actctttctt ttcttccctt acctaatctt
     7261 acatatgagt tatcgttttc atctaccata aggtctttcc actttaagct aactaattca
     7321 tttaataaac acccagtagt aactagacag actattatta ctctatctct tatatttaat
     7381 tttggtagga tacctataag tttatttatt tcttgtatac ttagaacgtc atcttttgtt
     7441 tttattcttg ttactgttgg tttttctaca tatctaaatg gatttatatc tatgtatcct
     7501 tcttttttta agaagttata aaaactatgt aaataactat atatcttttc acaagttgat
     7561 ttcgcatatt tagtctgtat ataatctaca aaatttttac aatcttcttt tgtagcataa
     7621 atcaattcct tcccttttaa aaactcttta aacagtataa cctttgataa ataatcgtgt
     7681 tttgtgtttg gatttaattt tgtagaaaaa acattaaaag tttcatactc ataatcatct
     7741 agtatttttg tactatacat aataactcct ttccttttca taaaataaaa taactatact
     7801 tttactatac catatatttc cccctcaaat atacccctta ttcaagggat gacttttaag
     7861 tttattttgt tatattatga aggttttatg aaaatgtcaa tcttccattg tgcactacac
     7921 agtactatgg tattataagt tagtaaaagg tggcattttt ttattaatat atgaataaaa
     7981 tatgctacta taaatttgta ttggaggtat tttggtttgg aaacatttag tttgcttgaa
     8041 atttttaagg cagtcatttt aggaatagtt caaggaataa cagaatggct accagtaagt
     8101 agtacagggc atatgatatt agttgatgag tttataaaat tgaatttttc aaatacattt
     8161 ataagtacat ttttagttgt aattcaattt ggttcaatat tagctgtact tgttatattt
     8221 tttagaaaat taaacccttt tgatagtgca aaaaatatta agcagaaaaa agaaacagtt
     8281 cgtttatggc ttaaggttat aattgcagtt ataccatctg gagttattgg aatactgttt
     8341 gaagatgata tagatagatt attctttaat tcaacagtag ttgctattgc tcttattgtc
     8401 tatggtataa taatgatagg gcttgaaaag agaaataaaa gacctaagta taaagatttt
     8461 tctcaagtta catataaatt agctctttgt ataggattat tccaatgtct agctcttata
     8521 ccaggtacat ctaggtctgg ttctactata ataggtgcag tattacttgg tacttctaga
     8581 tatgtagcag cagagttttc attcttctta gctatcccta caatgcttgg agctagtgca
     8641 ttaaaacttc taaaagctgg atttggattt actggatttg aatggcttat tttaggtgtt
     8701 ggttctgtgg tagcatttgt tgtttcaatt gtagttatta aattttttat ggactatatc
     8761 aaaaaacatg acttcaaagt atttggttat tatagaatag tcctgggtat agtggttctt
     8821 gcttatttct ttctattata atgtattcta atttaataac aaatgataag gagaaaagtt
     8881 atgagtgtta aaaaagaaat atttgattgg attaagtcaa tagctatggc tattgtactt
     8941 gcatttgtaa ttctacaatt tataagacct tctattgtca gtggagaatc aatgtatcct
     9001 actttagatg ataaagatta cctaatttta aataggatat catacaaagt tggtaaacct
     9061 gaaaaaggcg atattgtagt ttttaaaacc aatttagttg atggagaaac aggaaagaaa
     9121 aaagacttaa taaaaagaat tatagctact gaaggtgaca gaataaaaat atcaaattct
     9181 aaggtgtatg taaatggaaa attattaaat gagccatata tacataataa ctatacttct
     9241 ggagatatag atactgttgt tccaaaaggt aaaatatttg caatgggaga taatagagaa
     9301 aatagtaatg atagtagatt tcctgatgta ggtatgattg atgaggatga aattcttggt
     9361 aaggttatgg tgagactatt acctcttgat aatattggaa aagtagatta aaaaaaagag
     9421 actgaattgg taaaatttat tttacaaatc tcagtctctt tttatttact taaattaaaa
     9481 atatttttta ctaaatatct tttgattctg taataagtct tattatatca cttgcttctt
     9541 cattaaaagt tgttactata cttaagtagt ctgttgctct actcatagct aaataaacct
     9601 tatttaaatc tcctacaaaa tcatttactt gataatcttg ctcaatatca tttatagttt
     9661 gattgtataa catttctaat tcacatacta ttactgcttt atattctaaa ttctttattg
     9721 catatatatt tgctattgta gttcctacct tcttacttat atttgttaag ttatcttctg
     9781 cacatatata aggaattttt gcctcttcta atgctttttt taacatatac tggaaataaa
     9841 tagtttttcc actttttaat ttcttcttat tataaggata aacaatagca acttcggaat
     9901 aatcaagacc ttttttactt attaaatact caatttccca tattacagaa cttatttggt
     9961 catcaaggtc acttactttt attatatcaa cagacttatt tccacctcta agtgctttag
    10021 tataaaagaa agtactaaat tctgtatttg gtcttaattc gttgatatat gagttagaat
    10081 tatttgaaaa tttatttgta aaatctacaa tctctttggc ttgtctataa tttttactta
    10141 agataatctt atcatcaaat tctataccat tatataactt tttaaatata tttaatctat
    10201 tagagatatt caaagagtta caagaacaca cattaaatat aaattttgtt ttatataaaa
    10261 actctcttat aaaatctatt tcatactcta aaaagctttc tgcttcatct ataaaaatag
    10321 ctttaaacat atttttattt tttataatgt tctgtgcttg ttttacaaga ttattaaacg
    10381 ccttttcgta atcatttttt aacatgttat agtctacaat taaattgaat tttttagcta
    10441 gtttaaatat aaaagaacta aatgtgtgaa cttctaaatt attattatct ttatataaca
    10501 actctatcct ctctctaagc tcattgcgca attgttttgt atgtgtaaat atgattaatt
    10561 tatgatgtgg atatactttt gctaatttta tagctctaga aagcataatt gctgttttac
    10621 ctataccaga gccaccttca ataagtgtat tcccatattt aatagaaata acatctttaa
    10681 tttgaacttc ttccatcata gttgctgtat acttatagtc atcattgtag aatgaaattt
    10741 ttttaaattt ttcatttaaa tgaagtttgt catttattat atagtattct gaacatacat
    10801 ctaataaaaa taaatttaag tttatctcat cattctcatc attaagatac tcatccagcg
    10861 aacctttatt cattatatct tgaagcttat tcttatctat tatattgtta ttaacaaatt
    10921 cttcaaattc atatgtttcc tcaacttcaa cgtatggcat aatgtaaacg tagttataac
    10981 ttatatttct attcttttgt cccattttta gtttcaaaag ttcatattcc tcattcataa
    11041 cttcaagtaa ctcttcttct aaaataaaaa atatatcttc cgttgtatcc ataaatttta
    11101 taaataaaat ttttccatct ttaacataca acaaatcggt atttattcct ttaaaaggag
    11161 taacttttgg cactacttta acgtccttat tgatattctc gtaaaatatt ttctcaaatt
    11221 ctttcaattt tagggcgtct aactgagaat ttatatttat gtattggctc acttacatta
    11281 cctccttctg ttatacttaa ttataccatt ttgaactata tttttatata tactttttat
    11341 aggcaaattt agtaaataaa ttcaaaataa atacaaataa atacaaaaca gctaaaaaaa
    11401 ttttgcaata agtacagata tttattgtta tagaatattt tatgtaaata gtaaaatgat
    11461 gaatttcata attaatactt acaa
//
LOCUS       DAMJVM010000110        11130 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.114, whole genome shotgun sequence.
ACCESSION   DAMJVM010000110 DAMJVM010000000
VERSION     DAMJVM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11130
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.114"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            169..1575
                     /locus_tag="PC523_003021"
     CDS             169..1575
                     /locus_tag="PC523_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2476521.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYNNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGIDFYNKII
                     DKLIECDIEPFVTLYHFDLPFTLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMRYLEKRNIVPHM
                     EDGDKEVLKKAKMDFIAVNYYRTNCAGAFPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACRLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            1603..2451
                     /locus_tag="PC523_003022"
     CDS             1603..2451
                     /locus_tag="PC523_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2476522.1"
                     /translation="MYITKVLNNSLLLARDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLELPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSKEKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            3111..3803
                     /locus_tag="PC523_003023"
     CDS             3111..3803
                     /locus_tag="PC523_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2476523.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEHIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKNDNYSKVHGLRAGADDYLTKP
                     FEIAEFTARVLSLIRRYTRFNPKNEESQQLSFKGLLIDLDQHTVTTNGETMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            3800..4735
                     /locus_tag="PC523_003024"
     CDS             3800..4735
                     /locus_tag="PC523_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2476524.1"
                     /translation="MKMDEQTIILFLFLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGNENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIGQEREEYIEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLDFEIDIPENRLMVNLDSDGYSRVVNNLVQN
                     VLAHSKARRIKITMSEDSKMVLLRVEDNGVGIAKENLQHIFERLYKCDKGRSKKGSGL
                     GLSIVSQMVERMGGKISVESEIGKYTVFIVSLPLI"
     gene            4885..5808
                     /locus_tag="PC523_003025"
     CDS             4885..5808
                     /locus_tag="PC523_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2476525.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGSGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRKFIRNLCTERGKTILISSHILSEIALLADDIGIIDRGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLEIQLNLKNFKVADNHNIMLYDTDVSVAEINRAF
                     IQNNIDVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            5792..6562
                     /locus_tag="PC523_003026"
     CDS             5792..6562
                     /locus_tag="PC523_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2476526.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIVFGIMFFVTALPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICIIYKKQEN"
     gene            6572..7354
                     /locus_tag="PC523_003027"
     CDS             6572..7354
                     /locus_tag="PC523_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2476527.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQIVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFVITLIGGYLINREYTDQTLKNILTIPISLRKLLVGKL
                     IAVGGITVLLSIFSFLCTFILGLLFCNINISVILVAKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNSFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLAGYNNDDGSVYQPLI
                     GVMTLVVILILTAILLTFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            7732..8268
                     /locus_tag="PC523_003028"
     CDS             7732..8268
                     /locus_tag="PC523_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2476528.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            8300..8689
                     /locus_tag="PC523_003029"
     CDS             8300..8689
                     /locus_tag="PC523_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2476529.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLSQLTEHGFIKKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLKELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            8726..9112
                     /locus_tag="PC523_003030"
     CDS             8726..9112
                     /locus_tag="PC523_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HDF2476530.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNSVTVKVSSAIHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            9239..10681
                     /locus_tag="PC523_003031"
     CDS             9239..10681
                     /locus_tag="PC523_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2476531.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDENIAEDIVEDEPKLNTEKDGDLNYVSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAKSKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGKDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            10978..11097
                     /locus_tag="PC523_003032"
                     /pseudo
     CDS             10978..11097
                     /locus_tag="PC523_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
BASE COUNT         4025 a         1395 c         2097 g         3613 t
ORIGIN      
        1 aagaaaataa ttaatataga ataaatctat tgcaatgaag taacaaagaa aatagttaat
       61 ataaagtgaa cttattgtaa tgaattatta aagaaaataa ttaatataga ttaaacttat
      121 tttaatgaag tacaaagaaa ataatcaata taaaaaggag aaatatttat gaatacagga
      181 tttccaaaag attttttatg gggagcatca tcttctgcat ttcaagtgga aggagcttgg
      241 gataaagata ataaaggaaa aaccgtagct gactataatt cttttaaaaa gtctcattta
      301 caggcagata ctaaagtggc atcagacttt tacaataatt atgaagaaga tattgaatta
      361 atgaaagaat tagggatgaa aacatatcgt ttttcaattt catgggcaag aataatacca
      421 gatggtgaag gagagataaa ccagaaaggt atagattttt ataataagat aatagacaaa
      481 ctaatagagt gtgatattga accattcgta actttatacc attttgacct tccatttaca
      541 ttagtagaga aatataatgg ctgggaaagt agagaaactg tctatgcttt tgaaagattt
      601 gctaaaatat gctttaagca ttttggagat agagtaaagt attggcaacc tcataatgaa
      661 cagaatttaa ttgttagagt ggaagagaga attaatatat atgatgaaac agattcatgg
      721 aaaatagata aaatgcgtgc tcaaatggac tataatcttt gtttagcaca tgcattagca
      781 gtcaatgcat gccatgagat gataaaagaa agtaaaattg gagcagcagt atcatcatct
      841 gtaacatatc ctcttacttc caagccagaa gatgtatatg cagctagaat gaatgataat
      901 tttaaagtgt actatatgct tgatatgcat cactatggag aatatccagg gtattatatg
      961 aggtatcttg aaaaaagaaa tatagtccct catatggaag atggggataa agaagtactt
     1021 aaaaaggcta aaatggattt tatagctgta aattattata gaacaaattg tgcaggagcc
     1081 ttcccagagg attcacaaca tccatttggg ttaagagaag gaactgttga ttttagtatg
     1141 tatggattat ttaaaatgtc aatgaatcct aacctagaag cttctgagta tggagcagca
     1201 atagacccat caggtcttag agttgcctta aatgaatatt ggcaaagata tcacttacct
     1261 gttataataa cagaaaatgg tcttggagct aaagacatat tagaagatgg aaaaattcat
     1321 gatgactatc gtatagatta tttaaggagt catataaatg catgtagact agctattgaa
     1381 gatggtgtag agatgattgg atattgtcca tggtcattta cagacctttt aagctcttct
     1441 caaggtttta ataagcgtta tggacttgtt tatataaata gaactgacca tgaagtttta
     1501 gatttgaaaa gaatcaagaa agatagtttt tactggtaca aagaagttat agagaacaat
     1561 ggaatagtaa aataaacata aatatatatt gagggtttta gtatgtacat tacaaaggta
     1621 ttaaataatt cgttgctact tgcaagggat gataatgatg aagaaataat attgatggga
     1681 aaagggattg gccataatta taaggctggg tctgagttaa ataaaaaaga tatagaaaag
     1741 atttttgttc ttcatgatga aaatataaaa aagagtatta tacaactggc aacagaaata
     1801 gatgcagaat attttggtat agctcaaatg ataattgcat atggaataga aaaatataac
     1861 ttaaaactta tgaaccatat atatttggcc ttaactgacc acattgcctt tgcagtgaga
     1921 cgctttaaag aaggtataat gattgaaaat cactatttgt ttgaaatcaa ggagtttaat
     1981 ccaaaagagt atgatatagg aaaatatgca attactgttt ttaaggaggt tcttggttta
     2041 gaactaccag aagaagagat tggtaatata gcaggccatt ttataaatgc acaacaagat
     2101 aatccttata gtgatagaaa taagagaagt gctaaaattg taaatgctat tttgcaaata
     2161 gttcattatc acttttctat tgtatataat aaagagtcat tttactatag gagatttgta
     2221 atgcatctaa aggccttttc acaaagattt ctggctaacg aaccgtcaaa agaaaaaata
     2281 gattttatat atgagcaagt tcaaaaaaat tgtaaggaag agtatgaatg tgtgaaaaag
     2341 gtaggagctt atatgtataa ggaatactcc agagaattac caagacaaga ggagttatat
     2401 ttgatgattc atattcataa gattttagga gaattatgcg aaaatagtta gaatatctta
     2461 atatgctagt aatattataa aaaattaaaa attaattaca ctttaaatat gaataattgc
     2521 attacttaag ggggttgtct taaaattgat ttttttaatt aaattttaag ttcaactcct
     2581 ttttaaacct ttatatcatt acaactttaa tgtttatacg cctgtacttc agatgtgtat
     2641 ctatttttta catttctcaa catgaaaatt tatcattttt tgtagtgtgt taattgaaat
     2701 aaacctttga gttaacccta aatttattct ggacctacag gaatagttcc aaagatactt
     2761 taaaatattt tctggaatga ttgcttattt catatatatt ttattgtgat gttttgattt
     2821 aataaattct tcaactccgt atatagtaaa tgtattatca tgtttgtttt atttaagcac
     2881 acttataata tgcttgattt tttcaaaatg atgaaatata ctaatgggtg ttggatgtat
     2941 aagaatcaca ttatacttga aaaagtaagt gagaaatgag gtataattcc tcatatgata
     3001 aactactatt gtattgtgga acatattctc agtgatgtca agatattaca atatgactaa
     3061 attcctaatg ataaaaaagt ctacgaatga gtgtagaaag gggaactaag atgaataaag
     3121 ttttaattat agatgatgat aaagatcttt gtattttgtt aaaaaagagt atctcaattg
     3181 aacatattga agcaaactat tgccattctg ggagagatgg attggttgaa ctttcaaaac
     3241 aagattatca gttagtagtt ctagacgtga tgatgccagg tatggatggt tttgaaacta
     3301 tggaacaaat acgaaaatat agcagtattc ccatcttaat gctgacatcg aaaaatgata
     3361 attacagcaa agttcatgga ttgcgagctg gtgcggatga ctatctaacc aaaccatttg
     3421 aaattgcaga atttactgct cgtgtactgt ctttaatccg acgttacacc cgttttaatc
     3481 caaagaatga ggagtcacag caactttctt tcaaaggtct gttgattgac cttgaccagc
     3541 atacagtgac taccaatggc gaaactatgt tgcttccacc caaggaattt ggtgtgcttc
     3601 tttactgtgc aaggcatcag ggtaagattt tgacaaaaca gcagatttat gaagctgtgt
     3661 ggggtgagcc atatgtatat gatgacagca atatcatggc tattatcagt cgcctaagaa
     3721 aaaaaattga actagaccca tcatctccca tctatttgca aacgataaaa ggaatcgggt
     3781 atcaattcaa cacggaggta tgaaaatgga tgaacaaacg ataattcttt ttttgttttt
     3841 gctgtcgatt ggtctacttt tcctttccat atttgctttt tccaagcttg gtcatattca
     3901 caaacgtcta aaagatatag aagaaatact tgctgatgta caaattggaa acgaaaatcg
     3961 aaaaatacta attaagccat gtgatgttat ggctccattg gtttatcagc ttaatgagat
     4021 tgtctacgat tatgaaaata agttactttc tctgaaaaag tcggataaag ctagcaaaca
     4081 acttatgaca agtttatctc atgatgttcg tacaccatta acgactctga ttggttatct
     4141 ggatgctgta cacagtggta tagttatcgg acaagaacgg gaggaataca ttgaaatcgc
     4201 tcggcgtaga gcctacgacc ttaaggatta tatcgatgtt ctttttgact ggtttcgatt
     4261 gaactctgat gaatttacgt tgtcaataga atctgtggaa attgcagagt tgtcacgtaa
     4321 tattcttaaa gattggattc ctatcttcca tgaaaagaaa ttggattttg aaattgatat
     4381 tccagaaaat cgactaatgg taaaccttga ttcagatgga tattctcgtg tagtcaataa
     4441 tctagttcaa aacgttcttg cccatagcaa ggctcgccga atcaaaatta ccatgagtga
     4501 ggattctaag atggtattac ttcgtgttga ggataatggt gttgggattg ccaaagaaaa
     4561 tttacagcat atttttgagc gactgtacaa gtgtgataaa ggacgttcaa aaaaaggaag
     4621 tggtcttggt ttatccattg tcagtcagat ggtggagcga atgggcggta agatttctgt
     4681 agaaagtgaa attggaaagt ataccgtatt tatagtgagc ttaccattaa tataaaaatt
     4741 gtttttcccc catgtgatat atatacacag gggggatttt ttataagcta taaagttgca
     4801 aggttaatac aaggttgttg caaggttaat gtaagattga ggtggtatga taaatttaat
     4861 aaacaaagga ggattttact atttatgaat aatttaatta ttgaaacaaa aaatctcact
     4921 aaacaatatg gtgctcaaaa gagcgtttct aatttgaata ttcatgttca gaaaggtcgt
     4981 atttatggat tgctgggaag aaatggttcg ggtaagacga caactatgaa aatgttattg
     5041 ggtctaacaa aaccaactac tggagaaatt aaaatatttg gtcaggatat tcataataac
     5101 gagaaaaagc ttcttcctag aattggatgt ttgattgaat caccaggttt ttatcctaat
     5161 ctgacaggaa cagaaaatct caaaatcttt gcgaggctta gaggaatacc aaaaaaaaat
     5221 gcagtagaat cagcactgga tgtagtcggt ttgccaaaca atgacaaaaa gttattttct
     5281 caatactctc ttggtatgaa acagaggttg gcaattgctc ttgccattat gcatgaccca
     5341 caattgttga ttttggacga gcctatcaat ggacttgatc ctattggtat tgcagaagta
     5401 agaaagttta ttcgtaatct ctgcacagaa cgaggtaaaa ctattttgat ttccagtcac
     5461 attctttctg aaattgcttt gttggcagat gacattggca ttattgaccg tggcattttg
     5521 ttggaggaag aaagccttgc acaactggaa gaaaaaaaca gtaaatacat acattttgct
     5581 gtatctgatg cagcacaagc agcaagactt ctggaaatcc agcttaacct gaaaaatttc
     5641 aaagtggcag acaatcacaa tattatgctt tatgatacag atgtctctgt tgcagaaata
     5701 aatcgtgctt tcattcaaaa caatattgat gtatcagaat ctcatttgtg cgaagatact
     5761 cttgaagatt acttcaagaa agtaacaggg ggtgaaggaa ttgcttgata taatttcatg
     5821 cgaattttca aaactcaagc ggttaaaatt tatactgatt tctattttag gtgcttgttt
     5881 atttccaatt cccgcaacaa ttttgattgc aaaggacaac ttgccttttg agcaattgtt
     5941 caaattagtt gtcaattttg gatatttttt gctattacca attgtattga gtattgttgc
     6001 tagccaactc tttttcatag aaagagataa cgatgtatta aaaaatctag caacagtgcc
     6061 agtgccaaaa ggaaaactgg ctctggcaaa attagctgtg ttgctgttta tatcattgtt
     6121 ctactccgtt gcagggttag gagctactat cataggaggt ttcattgttg gaatagtaga
     6181 gggggttgct gtaaaattag gaatgagtat tgtttttgga attatgtttt ttgttactgc
     6241 acttcctgtt gtggtactca ttgtatattt caatcgcagc tatatttttt ctattatact
     6301 ttcttttgta tatgctattt tcaattttag tatatcctta aatattatca actttgaacc
     6361 aaataaccca ctgttaagcg tattacctgc tcctgttata atgcgttggt ggatggcatt
     6421 ttggggagat cccactggcg aatacacggc acttagacag ccatatttac tctcaactcc
     6481 tgcttgtgta ggtattttat gtttgataac aattattgca gtcctgttga tttgtataat
     6541 ctacaagaag caggagaatt aggaggtatc tatgcttgat ataattacta cagaatttca
     6601 aaaaataaaa agatataata ttctatggat tggtgttgtt gctgttctgt tttcagcact
     6661 attggcggtt tttcagattg taagttcaca cggcagtgac cctctaacgt atgagagctt
     6721 tgcaaatgga gttatatgga ataatttcag tcttagtttt ccatttgtga ttacgctcat
     6781 tggaggatat cttattaacc gtgaatatac agaccaaacc ttaaaaaaca tcctaactat
     6841 tcctatttca ttacgcaaat tgctagtggg aaaattaatt gcggttggag gaattactgt
     6901 attgttaagt attttcagct tcttatgcac atttatttta gggttactat tttgcaatat
     6961 caatatatct gttatactcg ttgcaaaatc cttagttcag attttgtctg ttaatctgta
     7021 ttgttatgtt gctgttttgc cgattattgc ttattttggt cgtaaacaaa attccttttt
     7081 gacaggcgta gggattgcat ttgtatatgg cttctgtggt gtatttgttg ctggaagaaa
     7141 tctaacagac ttttatccaa tcacagcagg tcttggtcta gctggataca acaatgatga
     7201 tggtagtgtt tatcagccac ttataggtgt tatgactttg gttgtaattc taatcctgac
     7261 agctattcta cttacattta cacctaatta cgataaagtt atgaccatat ctaagaaaaa
     7321 agggataaag cataaaaagt attctcataa ataacataaa tattgtttct attatttaag
     7381 ttgtcaggca gtgaaaagaa aaaatctgtc gtacagtggc tatatgctca tgtatttaag
     7441 aagctacagt tttgattttt cataggtgtg gcttcttttt gtatttatta aatcaacaac
     7501 tatcataata tttgcaatag gtatagtaac aagagataat actgtcatat gtgttttata
     7561 tattacatta agtatattct aaaatatatg ttttaaataa tatatttgaa aataagaata
     7621 cttgaataaa aaaaataata ttatatatag ttgacaaaaa gtggaaatta atatactata
     7681 taaatataga aatagtaatc attactaata ttaaaaaagg aggaattaat tatgaactta
     7741 aaaggaacta aaacagaaaa gaatttaatg gcaggatttg caggagagtc agaagcaaga
     7801 aataaatata catactacgc ttcaaaagct agaaaagaag gttataatca aatagctgct
     7861 atatttgaag aaactgctaa caatgaaaag gaacatgcta aattatggtt taaactttta
     7921 catgatggta tgccttcaac agaggaaaat ttaaaggatg cagcagcagg cgaaaactat
     7981 gagtggactg acatgtatgc gaaatttgct aaagaagcta gagaagaagg atttgacaag
     8041 atagcttatt tatttgaggc tgttggaaaa atagagaaag agcatgaaga aagatactta
     8101 aagcttttag aaaacttaaa tgaaggaaaa atatttaaaa gagatgaaga agttgtttgg
     8161 caatgtcaaa actgtggtca tgtgtatgta ggtactgaag caccagaaaa atgtccagta
     8221 tgtgaccatc caaaagcata tttcaatata aaagctgaaa attattaaaa attatcatta
     8281 ttaaaaagga atgttaacta tgaaattttc taaacaacga gaactgattt tgaatgaaat
     8341 attaaataat ccggttcatc ctactgcgga ttacctatat gaaaacttaa aaaaagataa
     8401 tccaaatcta agcttaggaa ctgtgtatag aaatttatct caactaacgg aacatggctt
     8461 tataaaaaag gttagtattc cagggtatcc agatagattt gatggtagaa tagataatca
     8521 ctatcatatc atatgtgaag tatgtggaga ggtatacgat ttagaatctg aagttcttaa
     8581 taatttaaaa gaattaatat ctgaggagac agacataaaa ataacatctt ataacataag
     8641 ttttaaaggg atttgtaata attgtaaaag atgtagccaa gtaggttagc ttttaaattc
     8701 taaaaataat taatgaggtg agtttatgtg tagcgaacaa aaatttttta tatgtgagac
     8761 ttgtggaaat cttgtaggaa tgatacaaag tggtggtgta cctattttct gttgtggaaa
     8821 acctatgaag gagcttgttc ctaatacaac agatgcagca gttgaaaaac atgtacctgt
     8881 gattgaagtg gatggaaata gtgttactgt taaagtaagt agtgcaattc atccaatgac
     8941 taaagaacat catatagcat gggtgtatct tatgactgaa caaggtggtc aacgcaaatg
     9001 tctagcagta gatggagaac ctgtagttaa atttgctctg aatgatgatg ataaagtgat
     9061 ttctgcatat gcttattgta atttacatgg tttatggaag gctgaactgt aatatttaaa
     9121 taaattgagt caaaaaggag gctataaatg tattttcata caaatagcct cctataaatt
     9181 tatataaatt cgagttttaa ttttatttaa tgtcaatata atataaggag aaaataatat
     9241 gtcaatttac aaatgtagcg tttgcggata tatttatgat gaatctaaga atgataaaac
     9301 atgggatgaa ttaagtgaag actgggaatg ccctgtatgt acaaaaggtc gtagttattt
     9361 tggaaagata agtactgttt actatgaaga agatgaaaat atagcagaag acatagttga
     9421 agatgaacct aaattaaata ctgaaaaaga tggagattta aattacgtaa gtacatattt
     9481 aagaagagat gatgaagttg agaaacatat ggatataatt cacgaaatgg ctgttacagg
     9541 aaaatctata atagaaccga tgagaactaa actaccagta atttcttggg atgatatttt
     9601 aattatgggt gctcaattaa atccattacc tttaaacgaa catgatgaag taaatacaac
     9661 aacaataata gggaaaaaag caaagaagcc tatgattata gaaaatccag tttatatttc
     9721 ccacatgtca tttggagctt tatctaaaga actaaaaata gctttagcaa agggtgccgc
     9781 taaaagtaag acagctatgt gtagtggaga aggtggtata ttaccagaag aaaaagaagc
     9841 ttcatataag tatatttttg aatatgtacc aaataaatat agtgtaactg aagaaaatct
     9901 taaaaattct gatgctatag aaataaaaat tggtcaagga acaaaacctg gtatgggagg
     9961 tcatcttcct ggtgaaaaag taactgaaga aatagcaaag gtaagaaata agcctgtagg
    10021 taaagatgta ataagtccat cttgttttga ggaaatacaa tccaaagaag atttaaagaa
    10081 acttgtagat gaacttagag aagtttcaga aggtcgtcca ataggtgtta aaatttcagc
    10141 tggtcatatt gaaaaagata tggaatttat agcttatgct aaacctgatt ttgtgactat
    10201 tgatggtcgt ggtggagcta ctggagcaag cccaaagttg cttaaagatg caacttcaat
    10261 cccaacaata tttgcacttt atcgtgctag aaaatacata gatacacatg gattagatat
    10321 agatttggtt ataacaggtg ggcttcgtat atctactgat tttgccaagg caattgctat
    10381 gggtgctgat gcagttgcta ttgcaagttc tgctcttatg gcagcagcat gtcaacaata
    10441 tcgtatatgt ggttctggta agtgccctgt aggagtagct actcaggatg aggaattgag
    10501 aaaaagatta catatagaaa atagtgctaa tagagtggca aactttttaa atgtaagttt
    10561 agaagaactt aagacttttg ctagaatttc tggtcataaa gatatacatg atttaagtgt
    10621 tgatgattta tatactgtta actctgaaat atcaaattac acaaacatac aacatgtata
    10681 agttgaagtt tgagactatt agattgtaga tagttataaa agtaaatgat aaatatattt
    10741 tataaaaagg agtgtcttaa aatgaacttt ttagtttata agtcactctt ttttgtatga
    10801 agccaaatat atttatcatt ttaacaatga aaaagaatct tatatcttta ctttgagata
    10861 gattccttct atttatgaaa attaaagata ttaaattgta aaacattctg tgatgtcgta
    10921 ctattaaact aaagatgctg tactttaaag taaataaaaa tttaaggagt gattattatg
    10981 gaaaaattga agattcaagt tctagttgac aataatacat atattgatag gtactttgtt
    11041 ggagaaccag cagtaagcta ttacattgag attgatggaa ataggatttt gtttgattaa
    11101 aaaatagcta cgaacacttg tgactttagt
//
LOCUS       DAMJVM010000111        11039 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.116, whole genome shotgun sequence.
ACCESSION   DAMJVM010000111 DAMJVM010000000
VERSION     DAMJVM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11039
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.116"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(91..408)
                     /locus_tag="PC523_003033"
     CDS             complement(91..408)
                     /locus_tag="PC523_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476532.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLELLYRYVTGLEYIAIGLFTRRLDITIITIILVSTITTALYYLIRKKY
                     ITI"
     gene            complement(786..986)
                     /locus_tag="PC523_003034"
     CDS             complement(786..986)
                     /locus_tag="PC523_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476533.1"
                     /translation="MCFKTYILKRVLNNKHLEITRSFDLDNLICTATEILKDNEIDLI
                     ITDEKNNVVWRNEKSTPQQENA"
     gene            complement(1005..1418)
                     /locus_tag="PC523_003035"
     CDS             complement(1005..1418)
                     /locus_tag="PC523_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476534.1"
                     /translation="MKVKSKRSFIVGIIVCMLCCASLIIYCILKEKRFLISSFLLIAI
                     AIFNFCNAFSRKGIVEELHDSTDERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLIVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(1402..1635)
                     /locus_tag="PC523_003036"
     CDS             complement(1402..1635)
                     /locus_tag="PC523_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2476535.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            1747..1914
                     /locus_tag="PC523_003037"
                     /pseudo
     CDS             1747..1914
                     /locus_tag="PC523_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984867.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            2500..3192
                     /locus_tag="PC523_003038"
     CDS             2500..3192
                     /locus_tag="PC523_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2476536.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIMGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            3388..5460
                     /locus_tag="PC523_003039"
     CDS             3388..5460
                     /locus_tag="PC523_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2476537.1"
                     /translation="MEKYRALKLVSLIIISISFPLTILLATIGLAEPKYNIQKTSTKS
                     GIFNISNDFYDSNIFDEGTVASLLSNFKISVDSQLTSEEKSKFQTKSKSIDLSSNDSE
                     YDKIMINKQASEEYLGYLKNIKVYVINTYTGVFYTNTSYDNLNDFKQETKNYCNIEII
                     NNVGKSSYIKTINGEKFELNNLSMELNGVDEAFEAYVSFPEEPTMEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLFIILYSRIKCEVLKRHNVFLLIYSKIPMEFNIILVS
                     ISIAILTIYALAVSYHIDVIVLVSLSLLIVSNNIFFINVQLSYLENKMYIFKTSIIVR
                     VILWLIRTVREVSNAIKKVSLARKVIVIVIISLALIMVISARSISMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVDLQGGKFYIEIDGDLFKANLEFQKYEEEV
                     TNMEKDGI"
     gene            complement(5653..5976)
                     /locus_tag="PC523_003040"
     CDS             complement(5653..5976)
                     /locus_tag="PC523_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDF2476538.1"
                     /translation="MNSNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLDLSIANPKIISFMIVLLVAYKFKSIGISIITGVVSIYL
                     FGIIM"
     gene            complement(5969..6670)
                     /locus_tag="PC523_003041"
     CDS             complement(5969..6670)
                     /locus_tag="PC523_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDF2476539.1"
                     /translation="MERTQQLRESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEDSYE"
     gene            complement(6948..8333)
                     /locus_tag="PC523_003042"
     CDS             complement(6948..8333)
                     /locus_tag="PC523_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2476540.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIVKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTADSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGVQLIPIK
                     QEHNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYRESLSDALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPEGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESIEELKAGL
                     SILKRLLEEKK"
     gene            complement(8437..9675)
                     /locus_tag="PC523_003043"
     CDS             complement(8437..9675)
                     /locus_tag="PC523_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2476541.1"
                     /translation="MELFIQVIKFNIYISPLILFFILFTKKISKYTFKFNYFICLLII
                     VRMLFISKINLNILNINNSTINIYKHNINNYIPYSQDIILDIFMYIWILGIILISFHY
                     LRTNLKFYNYVENFKKEILEIDITSILDEQVKELNINKKLKIYKVKGVYSPAIIGLTC
                     CEIIIPEKKYNNKELRFIFKHELIHYKRKDNLFKLLISIICIIHWFNPILYLLKKYFN
                     ELCELSCDEIVIDKCSISEIKEYAYIILDTIKYQKTLKYSSFVSQFLNKKNITLKKRL
                     NYMFNQDKKKINLGFKLLLTFIIIGSIFTINSNFKQNEDIEDTTGKINYNKNGETYGT
                     DIIDSNGNSIEPELIQAIGRGKKVGYVKKRDLYDCDNQPNTPEEAVLYTRKRNFNIFK
                     ITIPLYDKEGVKIIGRFEIN"
     gene            complement(9688..10065)
                     /locus_tag="PC523_003044"
     CDS             complement(9688..10065)
                     /locus_tag="PC523_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2476542.1"
                     /translation="MIILKKIPKSELLVMNFIWDKGIDKIPAREVANYMENSHGWTKG
                     TTGKVLSRLSDKGFLEFEKDGRNTIYTIKVKHEEYLKFETKDFLELIHKNSIHSFISS
                     LGCENEMSDDDIKELEQWLKKMK"
     gene            complement(10559..10981)
                     /locus_tag="PC523_003045"
     CDS             complement(10559..10981)
                     /locus_tag="PC523_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDF2476543.1"
                     /translation="MNTFKIEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESIITRYELIHIDLENTKLIFTENMSERNVF
                     GKINSVLVRILFGKRQPEEFNKFIKNLEMELENNSVNL"
BASE COUNT         4174 a         1315 c         1423 g         4127 t
ORIGIN      
        1 tttagtgaaa tagaaaattt agtaaaatag attagaaggt agcaaccgta actagttgct
       61 accttctctt agtattaatt ttattagatt ttatatagtt atatatttct ttctaattag
      121 ataatataat gctgttgtta tagtagatac taatattatt gttattattg taatatctaa
      181 tcttcttgta aataagccta ttgctatata ctcaagacct gttacatagc gataaagtaa
      241 ttctagttta acagtttttt tcttatctat atttccatat attgattcta atttattgaa
      301 atatttttta ctacaaaaag cagttttagg tttaattaaa tatattattg tagttcctat
      361 tataactatt attccaataa gtataagatt gatatttcca aaatccatag tagaaacctc
      421 ctgttagatt tgattttctt aatcaaagta tatcagtaac acaagtataa agagtatcta
      481 aaatattaca aatattaaca ggacgcataa aactatatag tataagttgc aataataaaa
      541 ctaacgaaaa ttatcatact aaaacataag acacgttata atcatgttag agaaattctt
      601 gaatatacac taaaagtact ctttctatag gagtgcttct ttttttaaat ttattaatgt
      661 ataaactatc aagactgtta ctcaaaaccc tttaaaattg atttaaatag ctctttttaa
      721 caacataatt tatatgatat aataaaaaat agaagtgtat tcgcagtaca cttctatagt
      781 tgtaattaag cattctcttg ctggggagtg cttttttcat ttctccaaac aacattattt
      841 ttttcatctg taattattaa atcaatctca ttatctttca aaatctctgt tgcagtacaa
      901 atgagattat ctaagtcgaa acttcgagtt atctctagat gtttgttgtt tagaactctt
      961 ttaagtatat aagttttaaa acacatttgt atcacctctt gtatttactc ttttttctct
     1021 aaaaatatat tgataagtaa atatgcaaca aataaaaaaa tctcaatgac acatagtgtt
     1081 atagctataa ctataagtga ttgatttttc catgcactat aagctatgat gaaaagaaat
     1141 gtaaaagtaa ataatgtata attcataatt ttaactaaga tatgacttgt tttcattgtt
     1201 aagtacaagt ctctttcatc tgtactatcg tgtaattctt caacaatacc ttttcttgaa
     1261 aaagcattac aaaaattaaa gattgctatt gcaattaaaa gaaatgatga aattaggaat
     1321 cttttctctt ttaaaatgca ataaataatt aaactagcac aacataacat acaaactata
     1381 atacccacta taaaacttcg tttactcttt actttcatgt tttttatcct ccatttcttt
     1441 attttccttt aaacaacata gttcttcaat agttgtacca aacacttgtg ccatacgata
     1501 ggcaagcata agagaagggc tatattgctc tttttcaata gaaataattg ttctgtttga
     1561 tacatggact aaatcagcga gttgttgctg tgttagaccc atatttgttc gaaattctcg
     1621 tacttttgtt ttcattccat aacctctttc tgtatgaagt taacttcatg tgaagttagc
     1681 ttcataatat cctaaaatga gtaaaatgtc aatcttatta gataagtatt ataaaaacct
     1741 aaaagaaaaa aatatctaag gtttgaaacg aaagaatttt tgaagaacat acataaaaat
     1801 tcaatttcaa gtttaatgtc agcgttacat ggtgaatacg acgaagtgga tgaagataaa
     1861 ttaattgaaa tcgaagaaca ttttaagagt ttgaaagaag aaaatgacag ctagatacat
     1921 aataaataaa atgaaggtat gaaattataa ctttttctac cttcattttg tttattattc
     1981 tgaacatgtt aagaaaatga aatagatttg ttttatattt atataaattt taatagaata
     2041 tgcatagtat atagtagata gtatgtataa aaatagaatt taaataagca gctattattg
     2101 cagcgaaata aatgtttcta agtatatttg ggtggaagaa aattagtgaa atgtaattct
     2161 tccaccactc ttccacccaa aaaataaaaa ctgttcaata agaattaata tacattaatt
     2221 cagctgaatc caatcaaaat acttaaataa aagtaatctg aaaacaacat aattcaaatc
     2281 aatttattca aataaacctc aatttctctt aaatcttaag aaatatttaa gaattaggat
     2341 aatattttct taagatttat attttattat agacttataa gagaaagtta caaaaatgta
     2401 agtcaaagta atataacaat ttagtcattg ttgagaaagg aagtgaaaga tattgtataa
     2461 tatagataag tattattata caatagagga agtgaaaaca tgtacaatat attagtagta
     2521 gacgatgata aagaaattgt tgattcaata gaaatatatc ttaaatcaga aggatttaag
     2581 atatataaag catatgatgg aatgaaagct ttggaaataa taacagagaa tgacattcat
     2641 ctaatactga tggatataat gatgcctaaa ttagatggaa taaaagcaac tgtaaaaata
     2701 cgagaagaaa gaaacattcc aataatttta atttcagcaa aaagtgaaga tacagataaa
     2761 ataatgggat taaatattgg agctgatgat tatataacaa agccatttaa tctattagag
     2821 cttatagcta gagttaaatc aaacttaaga cgatatgtaa ctttaggtaa ttatgaagat
     2881 acatgtaaag aaattttaaa aagtggagct ttggagttaa atacactaac aaaagaagtt
     2941 aaaattgatg gtgaaaacat aaaaatgact ccaattgagt ataaaataat tgaacttctt
     3001 ttagaaaata aaggacgagt tttctcaata gatgagatat acgaaaaagt atggaatgag
     3061 gaaagtttta actcagaaaa tacagtagca gtacatatca gacgaataag agaaaagatt
     3121 gaaataaatc caaaagaacc aagattttta aaggtggtgt ggggagttgg ttataagatt
     3181 gaaaaatttt agtggaaaaa ttataaaaga aaagagttta atatttaatt gcattttgat
     3241 tttagtcatt tttacattta cacaaatgtt aggaatttta atatgtttta attttttctc
     3301 tgagagttta atttactcta tggggattat gcttttctta aactttattt tatataaaat
     3361 actgacaatg tagatgaggt gaacaacttg gaaaaatata gagcattaaa gttagtttca
     3421 ttaatcataa tatctatctc atttccattg actatattgt tggcaacaat aggacttgct
     3481 gagcctaaat acaatataca aaaaacttct acaaagagtg ggatttttaa tatatctaat
     3541 gatttttatg atagtaatat atttgatgaa ggaacagtgg caagcttatt atccaatttt
     3601 aagatatctg tggatagtca gttaactagt gaagaaaaaa gtaaattcca aacaaaaagt
     3661 aaaagtatag atttaagtag taatgattct gagtatgata aaataatgat aaataaacaa
     3721 gcttctgaag aatatttagg ttatctaaaa aatataaaag tgtatgttat taatacatat
     3781 acaggagtat tttatacaaa tacatcatat gataatttaa atgattttaa acaagaaact
     3841 aagaactatt gcaatataga gataataaat aatgtaggaa agagctcgta tattaaaaca
     3901 ataaatggag aaaaatttga actgaataat ctgagtatgg aactaaatgg agtagatgaa
     3961 gcttttgagg catatgtatc ttttccagaa gaaccaacta tggaagatgg aatagtatac
     4021 actaattttc aaatatttaa acaagctaca gaaaaagtaa gattattttt tgtagctagt
     4081 ttaagtttag caattatatg tttattattt ataattttat atagtagaat aaaatgcgaa
     4141 gttttaaaaa gacataacgt atttttacta atatacagta agatacctat ggaatttaat
     4201 ataatactag tatctatttc aattgcaatt ttaactatat atgctcttgc tgtatcttat
     4261 catattgatg taattgtgtt agtttcttta tctttactga tagtatcaaa taatatattt
     4321 tttataaatg ttcaactgag ttatttagaa aataaaatgt atatatttaa gacaagtatt
     4381 atagtaagag tgattttatg gttaataaga actgtaaggg aggtcagtaa cgctataaag
     4441 aaagtatctt tagcaagaaa agttattgtt atagtgataa tatctttagc tttaatcatg
     4501 gtaattagtg ctcgaagtat aagtatggga ttactgtttt ctatatgtat ttttttagga
     4561 tttacaatat atataactaa aagactttca tatcttagtt acgtaataga aggtacagaa
     4621 agaataaaaa agggagaatt agattataag ataaaaatta ctgggaatga taattttacg
     4681 tctttagctg aaaatataaa taatattgga gaaggtcttg atagagctat agaagagcaa
     4741 gtaaaaagtg aaagaatgaa gtctgacctt ataacaaatg tatctcatga tttgaaaact
     4801 ccacttactt ctataataaa ttatgtagat ttaattaaaa aagaagaatc aatacaacca
     4861 gagtatataa atgattatat aaatgtgctt gagtcaaagt caaaaagact aaaattattg
     4921 atagaagatt tatttgaagc gagtagagta agtagtggca atatagaatt acaaattagc
     4981 aagattgatt tagtgcaact attaagacaa agtataggtg aacttgaaga aaaattatct
     5041 aaaaataatt tatatttaaa attaaatgtt ccaaacgata aggtgtatat atgggcagat
     5101 ggaagaagaa tgtacagagt atttgaaaat cttttatcta atatagcaaa atattcttta
     5161 gaagaaacta gagtctatat tgatgtatat gactatggtg aaaatgtaaa ggtaactatg
     5221 aaaaatatat cttcttatga attaaatttt gaccctagtg agattatgga aagatttaaa
     5281 agggctgatg aatcaagaaa tactgaaggt agtggattag gacttgctat tgcaagagac
     5341 ttagtagatt tacaaggtgg aaaattctat atagaaatag acggagattt atttaaagca
     5401 aatttagagt ttcaaaaata tgaagaagaa gtaactaata tggaaaaaga tggtatttaa
     5461 gtatacatac ttttgattgt aggaagctct tattataata tattagaata tttatagtag
     5521 aggatgaaga gtaaaaacag agtagattta agccactctg tttttttatt taaaatcaat
     5581 aatataacaa tttagattaa aattgtacat atgtttataa aataacttat aaaataaaac
     5641 attatctaaa tgttacatta taattccaaa taaatatata gataccactc ctgtaataat
     5701 agaaatacca atagatttaa acttatatgc gacaagaagt acaatcataa aagatataat
     5761 tttagggtta gctatagata aatctaggct atctccatta aaaaatacat ctttagcagt
     5821 taatgaagta agtattgcta caggtataaa tttcatgatt tttttcaaaa aatcaggcat
     5881 ctcttttttt gagaaaatta acataggaag cactcttggt agatatgtaa caatgtacat
     5941 tccaattata ataaaaaata tataattact attcataaga gtcctccttt ttacagtaag
     6001 attcttctat aaagaaacca attaaagatg ctaaaattga tgcaactatt attactaaat
     6061 tgcttttgaa taatgcatat aagataaccg aaataatgat tgatgatata aataccacaa
     6121 tataaatctt attttttatt tgagaaacta ttaaagctat aaacatagaa gtcaatataa
     6181 agccagaaat agaactgtct atagatatac tttctcctaa aaatgctcca ataaaattag
     6241 ctaaaactga tgaaattaag cagaagaaat tgagataaat agcatttcta tcagaccaat
     6301 ttccagcagt aaatttttca taattaactg caaaagtttc gtctgttata gtatgacaaa
     6361 atggcaatat aaattttgga cttttatttt ttatgtacat gttcaaatta gaactcatta
     6421 gaaaatgtct taagttaact ataaatgtag tcaaaacaat agatgttaca cttgcaccag
     6481 tagaaatcat tgaagctgca atgaattgag agcttcctcc aaatactaaa gttaccatta
     6541 gtgctatttg aaaaggtgaa agaccagatt tttgtaatag tattcctcct actataccta
     6601 taggtatata acctaagaat ataggaattg aagcaataaa gctttctctt aactgctgag
     6661 ttctttccac aataaatcac tccttttgta ttaaatgtat taaagtattt gtaaaacata
     6721 aaaatagatt aattttataa ttgcaattat aattaaaaat aaatttgaat taatatataa
     6781 attactgtat aacaaataga cagtcttaaa ctattattaa attttatcta tttgttacta
     6841 ttatgtaaca atacatatta atctctataa attaattact atctgttgta attttatttt
     6901 aacaacaaaa aaaaatacac acaattaaaa tattgtgtgc aatacaatta ttttttttct
     6961 tctaatagtc tttttaatat gctcagacca gctttaagct cttctatact ttctgtagca
     7021 catacagcta ttcttattgc agaaattggt ttttcttttc caacagcaaa acgttcagaa
     7081 gcataaacct gtacaccttc tttaagtgct tgtatttcaa atttttctgc cattattccc
     7141 tcaggaagaa tcaaccatct aaatatacat tcttcatttc ccaaaagatt atacccagat
     7201 aaatattgat taataatctt gtttcttctt cttgcagatt tacgacgtgc attaattaat
     7261 ttatcagctt cacctgatgt aatcatacga taagcaattt ctgttaaaaa ataagattga
     7321 gataaattaa tgttgtataa tgcgtctgat aatgattctc gatactgttt aggtgtagag
     7381 atatatgcta gccgtaaact aggtgcaata atttttgata aactcgtaat ataaattgtt
     7441 tggttaggaa gataggaagc tactggattt aaatgtgttt cattaagtaa actatgaata
     7501 gcatcctcaa taacaattaa attgtattta gatgcaatat ttgcaatcat ttttcgtcca
     7561 ttttgtgaca ttatatgtgt agttggattt tgataatctg gtattatgta tagaccttta
     7621 atattttcat ttttacaagc atacaatagt ccttcttcac taatttcatt atgttcttgc
     7681 ttaattggaa ttagttgtac tccaagcatt ttagctgctg tctttattcc tggataagtt
     7741 aatgggtcta cacctattct atctccatgt tgaaataaac ctgccaaaat agcaacaatt
     7801 gcattttgtc caccacttgc aggaagtaag ctatctgctg tagtttcaag cccagcttta
     7861 taaattaatt tggcaatagc ttctttttgc catttagcgc catctctttg tccatattgg
     7921 aatatagttt taaaatcgat ttctttaact atgtgtttaa ataaagtatt catttcttct
     7981 agtgtaaaat ctgggttcat tgtacccatt tcaataaatg taattttatt attactggac
     8041 attaagaata ggtttgaacg tgtatcataa gatacaaaag ttccacttcc tgtaactcca
     8101 cttatgagac cttttttttc acaaatttta aaagctctag agatagtact cacatttaca
     8161 tctaggaaat ctgccaattc acgttggggt ggcaatttag tacctggcaa taaaaaccca
     8221 ttgttgatat cttgttctag ttgttctgca agggccttat ataatatctg accttttgaa
     8281 ggtctctttg gcttccagtt cataggataa ttttcaaaag aattaattgg cataagtttt
     8341 cacctcacgt tattagttta atttttttct ttatctaaaa caataaattc taaggataca
     8401 aataaaaact gataggtaat aattctatca gttcttttaa ttgatttcaa atcttcctat
     8461 aatttttaca ccttctttgt cgtaaagagg aattgttatt ttaaatatat taaaatttct
     8521 ttttcttgtg tataaaacag cttcttctgg tgtatttggt tgattatcac aatcgtacaa
     8581 atctcttttt tttacatagc ctactttttt acctctacct attgcttgta taagttctgg
     8641 ttcaattgaa tttccatttg agtctattat atcagtacca taggtttctc catttttatt
     8701 ataattaatt ttaccagtag tatcttctat atcttcgttt tgtttaaaat tagagttaat
     8761 agtaaatatt gaccctatta ttataaaggt taacaaaagc ttaaaaccta aattaatttt
     8821 ctttttatct tggttaaaca tataattcaa cctctttttt aaagtaatat tttttttatt
     8881 tagaaattga gatacaaatg atgaatactt caaagttttt tgatacttta ttgtatctaa
     8941 tattatatat gcatactctt taatttcact aatgctacat ttgtcaatca caatttcatc
     9001 acaagataat tcacatagtt cattaaagta tttttttaat aagtataaaa ttggattgaa
     9061 ccaatgtatt atacatatta tactaattag aagtttaaat aagttatctt ttcttttata
     9121 atgaattaat tcatgtttaa agataaatct taactcttta ttattgtact ttttttctgg
     9181 aattattatt tcacagcagg ttaaacctat aatggcaggg gaataaacac ctttaacctt
     9241 atatattttg agttttttat ttatatttag ttcttttact tgttcatcta agatactagt
     9301 aatatctatt tctaaaattt cttttttaaa attttctacg taattgtaaa atttaaggtt
     9361 tgttcttaaa tagtgaaaag agattaatat aattcctaat atccagatgt acataaatat
     9421 atctaaaata atatcctgac tatatggtat ataattattg atattatgtt tatagatatt
     9481 aatagtgcta ttattgatat ttaatatgtt taaatttatt tttgatataa atagcattct
     9541 gactattatt aaaagacata taaaataatt aaacttaaaa gtatatttgc ttatcttttt
     9601 agtaaataaa atgaaaaaaa gaattagtgg acttatgtaa atattaaatt taataacctg
     9661 tataaataat tccataaaat atgtctccta tttcattttt tttagccatt gttcaagttc
     9721 ttttatgtca tcatctgaca tttcattttc acaacctaat gatgatataa agctgtgaat
     9781 agaattttta tgtataagct ctaaaaaatc cttcgtttca aattttaaat attcctcatg
     9841 tttaacttta atagtatata tagtatttct accatctttt tcaaattcta aaaaaccttt
     9901 atcacttaat ctagaaagta ctttaccagt agtgcctttt gtccatccat gagaattttc
     9961 catgtaatta gcaacttctc tagctggtat tttatctatg cctttatccc atatgaaatt
    10021 cataactaac aactcagatt tgggtatttt tttcaatata atcactccct ttaacttgat
    10081 tatattagac aaatgtctaa cagtcaatat aatatatcat catttataaa gaatatctat
    10141 attattttat acttaagaac taaatattat atatttatag ataaaaaaga tattaagagt
    10201 tttatggaat agaataatct acttcctact agatataaga atataacata tattttttag
    10261 tgaataaatt aatatctgtt ctaagttaga ataggataat cttattagat aaagattatc
    10321 ctatttattg gaagtttaag atgtattgga gattattaat ttttttcttt ttttaactaa
    10381 aagcaataat acataaaatt attattacta atgaaactcc taatgtggta tatagtcatg
    10441 gtgttatatg actatcaata gttttttaag taattattgt aacaaatagc agtatcccaa
    10501 aaatacttaa aaatatgttt ctagacagtt tttaaatata atataatgta acttatatct
    10561 ataaattaac actattattt tccaactcca tttctaggtt ttttataaat ttattaaatt
    10621 cttctggttg cctttttcca aacaatattc ttacaagaac tgaattgatt tttccaaata
    10681 catttctctc gctcatattt tcagtaaaaa taagttttgt attttctaaa tcaatatgta
    10741 tcaattcata cctagtaata atactttctc ttttattgct tgtttttatc tcatatactc
    10801 tatttttttc gaaattagtt atttcaacct taaatctttt attatttttt tgttgtacaa
    10861 atgtgcccac tgcttttttc tcattaaaat taggaaaatt tagtcttgca gattttgtaa
    10921 atatattaaa tatgtctttt gcagaatatt ttaataaaac ttcaatttta aatgtattca
    10981 tatcgttttt cctcctgtat ataaatttaa ttttataagt taatttttaa gttaaaata
//
LOCUS       DAMJVM010000112        10727 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.117, whole genome shotgun sequence.
ACCESSION   DAMJVM010000112 DAMJVM010000000
VERSION     DAMJVM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10727
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.117"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            77..358
                     /locus_tag="PC523_003046"
     CDS             77..358
                     /locus_tag="PC523_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476544.1"
                     /translation="MASKQTPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
     gene            623..877
                     /locus_tag="PC523_003047"
     CDS             623..877
                     /locus_tag="PC523_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476545.1"
                     /translation="MENNDFKLTICIFAFVTGVVVLFCCIGLGEFSVEHIIQQNGGTM
                     DTDQYNIYLDQSISQYRNFGSILALLGGLGILFDKYIRKY"
     gene            922..1245
                     /locus_tag="PC523_003048"
     CDS             922..1245
                     /locus_tag="PC523_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476546.1"
                     /translation="MATEIFTVKITPIEAQKIIKENLNADLVFSDSYNLGNDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKAISTGSAIGVLKIDWGAGGNLLKRVKNIL
                     ADYII"
     gene            complement(1515..1709)
                     /locus_tag="PC523_003049"
     CDS             complement(1515..1709)
                     /locus_tag="PC523_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2476547.1"
                     /translation="MINYMIEIIYSEKISEVENILKIENIQYEFTSRDGFIFFGFYDM
                     TDAYKFETLCSDEKIQFKRI"
     gene            complement(2081..3700)
                     /locus_tag="PC523_003050"
     CDS             complement(2081..3700)
                     /locus_tag="PC523_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDF2476548.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPILGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLILNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFKNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            complement(4009..4749)
                     /locus_tag="PC523_003051"
     CDS             complement(4009..4749)
                     /locus_tag="PC523_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2476549.1"
                     /translation="MKDFKDDIELIINDFFDCCNIPTKVFSKDLKEICSIGYDPTLET
                     YFSSLNIFSNINSTDFISNKNLFYIMLSFCDDLHFLVMPIHKFDVSSGYFIVGPFKSC
                     INNDIEFSNIPFKPLSCLDYISNLLSEICLDKMKHRPALSFYVKKTIDYIHKNYSEDI
                     TVSDICNDLQLNKSYFCSLFKKESGYTFTNFLNKVRVEKSKKYLLEKDLSILDVAVLV
                     GFNSQNYYSMVFKKFNNLTPIEYKNIYN"
     gene            4898..5647
                     /locus_tag="PC523_003052"
     CDS             4898..5647
                     /locus_tag="PC523_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2476550.1"
                     /translation="MKKIAIYGKGGIGKSTTTSNLSASLSHLGYKVMQIGCDPKSDST
                     KNLMKGKFIPTVLDVLKEKKEELKLEDIVFEGYGGVLCIEAGGPTPGVGCAGRGIIAA
                     FEKLEELKAFEIYNPDVVIYDVLGDVVCGGFSMPIRNGFANEVYIVTSGEMMSMYAAS
                     NISLAVDQFKNRGYASLKGLILNAKNVENERELVEKLADEIKSEVFHYIPRNKIVQQS
                     ENNGRTVIEEDENNEMSEVYIELANKIMKSN"
     gene            5689..6579
                     /locus_tag="PC523_003053"
     CDS             5689..6579
                     /locus_tag="PC523_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2476551.1"
                     /translation="MKLKKLCSVFLLSSLILTGCSKASDGEAKEINNNVNVVSATVAA
                     TQVLDKLDATLVGIPTTKSDLPEKYKNLPEVGQAMNPDLEIVASLKPDVFIVDSMFKE
                     NVEKSMKEYNLDTFYFETGTYTQFLKSIEDLGEKINKQSEAKKLIDELEGVEQSVAEK
                     AKKQDKKPKVAILFGGGNNFMLATKSSYLGDLVKTVGAENITDNLTDNVESDYIQFSL
                     EQILKENPDYILRFAHGNIEETKKAFDDSFDKNPAWATLDAVKNGKVIDLDSSIFNVS
                     ANIHVKDSINKLGDILYGDK"
     gene            6596..7576
                     /locus_tag="PC523_003054"
     CDS             6596..7576
                     /locus_tag="PC523_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2476552.1"
                     /translation="MDNKKILLIVTGLITIIVLLILFSTIGSVNLSLREIFDALINEN
                     NPMVTTIVYKMRLPRNILAALVGANLAVSGLLLQAVMKNPLADPGITGISTGASVAAI
                     IILLLFPQYTNILPIVAFIGGLISCAVVYLMAYKNGLRPERIILAGVAVNTILGGVIS
                     YLSTMYSERIQSAMLWLNGSLATKTWLDVNMLIWYSIVGLIGAIFLIRSANVLQLGDD
                     AATNLGFNVNKSRFIISLVAIFLAGVSTSIVGVISFVGLIVPHITRMLLGSDHKYTIP
                     FSIILGSIVLLIADTLARTIGGSIEIPVGVIMSIIGGPFFLYLLRKRGSY"
     gene            7576..8370
                     /locus_tag="PC523_003055"
     CDS             7576..8370
                     /locus_tag="PC523_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2476553.1"
                     /translation="MFNVKNIKIGYEDKIIIDNLSVSIKKSEIVSILGPNGSGKSTLL
                     KSLSRILKIKQGDIYIGKKNMKVMSNKEISRQVALLAQHNVSLKDIKVKDLIYYGRMP
                     HKGFFEGKNRNDDEIVTWAMRNTGLESYKDKLVSNLSGGERQRVWLAMALCQKPDILL
                     LDEPTTYLDISHQLELMELVRDINKKFKMTIIMVLHDINQASKYSDRLIIMNNGSIVA
                     DGHPNKVISEKIIKEVYKVKCDIDIDPISKKPRIHPIMLCRRTSSI"
     gene            8469..9746
                     /locus_tag="PC523_003056"
     CDS             8469..9746
                     /locus_tag="PC523_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate:formate antiporter"
                     /protein_id="HDF2476554.1"
                     /translation="MYTLDVLNRLKDIDCDKDIKSLSYAVFPGTHCPLFGVVLTASYI
                     RNMALVVVGTNECTYYSKNFAYHRQFGNDSVYSVVLKDKDIVFGAEKKVVEAVKYISE
                     IGEFDAIMIVTTCVPELIGEDYNALSSELEDMLRIPVLTVNTEHYTCNSHILGMTRAL
                     RSLSSVMESYKKKEGVNILGHRQENVEDTELVKLLVKKDIKINCVIPSKCTIKDIKNA
                     SSAKLNIVTDMIALDLAEDMKNKFGIEYVYFDKNMKESIIIENYNKLCKILDIDIQDE
                     VSKQRQRYNSLLNECRYLLDGKKLIYGNTPMMAFETVDFLTELGLVPEFIQVRELYEQ
                     DNLFKDNIIKKGYNPYVSRIANIAPLRSLYENINADIYIGHENPMLLREKGLMQITLD
                     EHAQKIGFELPIGIMKTLIKLFDVEKSTRREVI"
     gene            9748..10692
                     /locus_tag="PC523_003057"
     CDS             9748..10692
                     /locus_tag="PC523_003057"
                     /inference="COORDINATES: protein motif:HMM:NF012375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HDF2476555.1"
                     /translation="MEIYRYFPNPSDRMGIIFIVSSINEACVIEFGPSGTTHYAIEAI
                     GSLNGEDKAKIYSTHMSESDVTFGNYDRLEKAIIEVDSNIKPKYIFVMASSVSSIIGT
                     DIIGICNILKESVNCRLIPITTGGLRDDYNQGVEEFLYILAKEVVKESSEKFDSYNII
                     GCTIDQFNFLADCEEIKRMMKAFFKKEVNVTFTSYTSIDEIENASKSSLNIVLRKEGI
                     KAAIFMKEKYSIPYVYKKPYGIKNTEEFINEIQKVTEWDLDTNTYDDEISNIKRYIFN
                     VKRKLYFYEGSKKCAVFGDYDTALGFRDLLEELGLKID"
BASE COUNT         4257 a         1166 c         1711 g         3593 t
ORIGIN      
        1 aataaataca tataaagata tttttaagaa taaaatttac tattaaataa aaaataaagg
       61 agagtgattt aatagtatgg catcaaaaca aacaccaaga aacaatttgg aaaagttttt
      121 aagaataaga gtcttatcac atacaggaga taaaatacat ataaagttac ctgtaaattt
      181 tgcaaagaga atgattgaga ataatgcttt ggatttattt aatggaaaag atgatgtagt
      241 agatggtaaa aaagttactg aaatattagt caaagcattt gactatgatt taactggaga
      301 agtagttcac ttagagagaa aaaatggaga tattattaaa gtttcaatcg tacaatagaa
      361 ttattttata tgaattttat ttttaaatag ttataaataa ttagaaattt gtaaaaaagt
      421 ataatatatt attagtaatg atgtagaata cacatttata atttatagat gtgtattttt
      481 tgtttgaaaa atttaaatat ataaaactat aaaggttaca attttggaaa tagatgtaaa
      541 tatatgtttt atagtataat aagaagtaat ataccatcta gtaaaaataa ttaaataaat
      601 ttaatttatg agagggataa ctatggaaaa taatgatttt aaattaacaa tatgtatatt
      661 tgcttttgta actggagtag tagtattatt ctgctgtata ggtttaggtg aatttagtgt
      721 agagcatatt attcaacaaa atggtggaac catggatact gaccaatata atatttatct
      781 agaccaatct atttctcaat atagaaattt tggttctata ttagcgttgt taggaggact
      841 agggatattg tttgataaat atatcagaaa atactaaaga ttatgttttg tttaaaataa
      901 atttttatga ggagataaaa aatggcaaca gaaatattta ctgtaaaaat tacgcctata
      961 gaagcacaaa aaataataaa agaaaatctt aatgcagatt tagtttttag tgattcttat
     1021 aatttaggaa atgataaatt tatactgatt acaacttttg agaagtatta tgcaagaaac
     1081 aatagtgatg cagggttaat cgtagtttat gaaaatacaa caggaaaaac agttattaaa
     1141 gctatttcaa caggtagtgc aattggagtt ttaaaaattg actggggtgc aggtggaaat
     1201 ttacttaaaa gggtaaaaaa tatacttgct gattatatta tttaattatt ttatctaatt
     1261 gataacatgt caagaaaatc tcgtaaaaga ggttaagaac attcttataa tatatagata
     1321 aacaagaatt taaaggtaaa gcttttaatc tataataaat aatattaata tagtatgaat
     1381 aaaaagtagg agcatattaa tataatgccc ctactttttt attattaaaa ttgtttataa
     1441 tattaaaact acattaaaat acagtctaaa tttaacaaat aaatggtgta gaaatatgta
     1501 ctttttttaa tttattatat tcttttaaat tgaatttttt catctgaaca taaagtctca
     1561 aatttgtatg cgtctgtcat atcataaaaa ccaaaaaata taaagccatc acgagaagta
     1621 aattcatatt gtatattttc aattttcaat atattttcta cctcacttat tttttcacta
     1681 tatataattt ctatcatata atttatcatt gtagaatccc tcctattaat atgaaaatat
     1741 ccaaatatat aataacatat ttagatgata taatagtatc caaataaaaa ataaagttat
     1801 ttcattattg aatgaagaat taaaatgaag ataaataaag taaatattgg ttataataga
     1861 ttaaaaaatg gctatgtaca cttgtaattt tagtcatagg aggttaaagt tttatgaaag
     1921 aagtcgtata tatatggggt attctagtca ggaattgttt aataataaga taatactata
     1981 aaaatttgag tattatataa aaagtaagaa gccataaatg atataagcat tatctatagc
     2041 ttcttatttc tcattgatta agattatata aaaacaataa ctatattttt tgtttaagtt
     2101 ttaatacttg ttttactata tttgttgagt ggtcagaaat tctttctaga ttagtaagta
     2161 aatcaagata aagtacacca gcatcaattg tacaacttcc gttatttagt ctaatcatat
     2221 gtgaattttt aaaagttttt tctaaataat ttacttcatt ttcaacttct aaaactttat
     2281 ttgctaactc aaaatctttg acatctaaaa ctattaaggc atccttgaaa ttagtaattg
     2341 ttttattata tatttcatat atttctctta ttgcactatc agaaaaacta atattcattt
     2401 ccttaacact catggccaat tcagctatat tttccgcatg gtcagagact ctttcaatgt
     2461 catttacagt attaaaaagt aaatctgttc taaaacgttc atcatctcta agaggttctt
     2521 tagaaagttt taataaatag tttaatatat ctttctgtaa cttattgaca actttttctg
     2581 attcaaaagc tttattaata tcctcattgc tcttatttaa aaatcctgca attgaatgtt
     2641 ccaaagagtt tgtagcttta tgacccattc ttacaacttc ttgtacagta ttaccaagtg
     2701 ctatagatgg agtactaaac atcctttcat ctaagtattt agtgttattt actatttctg
     2761 attcagtttc tttaataggt ataagtctat ttgctaattt aactatgtat ttagaaaatg
     2821 gtaataatat tataacattt gtaagattaa ataaagtatg tgcatttgct atttgtctag
     2881 ctgtatcagt tgggtcaaaa tgagtaacta tagctgatat aggtttattt aaaataagca
     2941 taaatattat tgtaccaatg acattaaaac ttaagtgcat tacagctgca cgtcgagcat
     3001 ttctacttgc accaacactt gataaaagtg aagtaacaca agtacctatg ttatctccat
     3061 aaagaatagg caaagcagat gatagaggaa gtattccttg agaagctaat gctattaaca
     3121 tacccataga tgcacttgaa ctttgaacaa taccagttat ggcgaatcct gccaatatac
     3181 ctaatatagg atttttacta aaatataaaa gtgcatcagt gaatgcttta tattcagcaa
     3241 gtggagctac tgcatctttc ataaactcca ttcctgtaaa taatatacca aaacctataa
     3301 gtatttcaga taaatgcttt gttttttgat ttgatgtaaa taaatagaat aaaataccta
     3361 tacctagagc aattggtgca attccttcaa gattaaatga aactaattga gcagtaactg
     3421 tagtacctat atttgccccc attattacac caatagcttg acttagattc attatacctg
     3481 catttacaaa tcctacaacc ataacagtag tagcacttga gctttgtata atacctgtaa
     3541 caactgtacc tactagtaca cccataacaa cattacttgt taagagttca ataatttttt
     3601 ttaatttgtc tcctgctgat ttttgtaaac cttctcccat taaactcatt ccataaagaa
     3661 aaagtccaag tccaccgata aggctaatta ctatattcac gatatttcct cccttaagaa
     3721 attaatgatt tttttatctg aattataaac tttattaact aaaatttatt gtttccataa
     3781 tatagaataa caaattttgt caataagaat attaatgttt tgttaagtat acgttaagag
     3841 aatgttaaat ttttaagttg ttgttaattt tagaaaattt atgatgtata gcaatttaaa
     3901 tacttggact tagattttta gtattttatg tacaaacaat ttaacaaaga attaactata
     3961 attgtaaaaa atatgtaaaa aaacagcatc tttttagaat gatatttttt agttataaat
     4021 attcttatat tcaattggtg taaggttatt aaacttttta aaaaccatac tatagtaatt
     4081 ttgactattg aatccaacta gtaccgcaac atctaaaata gacaaatctt tttctaatag
     4141 atactttttg cttttttcaa ctctaacttt atttaaaaaa ttagtaaaag tatatccact
     4201 ttctttttta aacaaagaac aaaaataact tttattaagt tgtaagtcat tgcatatatc
     4261 acttactgtt atatcttctg agtagttttt gtgaatataa tctatagttt tctttacata
     4321 aaaactaagc gctggtctat gcttcatttt atcaaggcat atttctgata aaagatttga
     4381 tatataatct aaacatgata aaggtttaaa tgggatatta gaaaactcta tatcattatt
     4441 gatgcatgat ttaaaaggac ctacaataaa gtaaccactt gatacatcaa acttatgtat
     4501 aggcataact aaaaaatgta agtcatcaca aaaacttaac attatataga atagattttt
     4561 attagatata aaatctgtac tatttatatt gctaaatata tttaaacttg aaaaatatgt
     4621 ttctaaagta gggtcatatc caatactaca aatctctttt aaatccttac taaatacttt
     4681 tgttggaata ttacaacagt caaaaaaatc attgatgatg agttctatat catctttaaa
     4741 atctttcaaa aaatcacctc atttaaattt atacaaatat ttttataaaa aaacaaatat
     4801 agttaagaat aaaaaaaata tatcaattaa aatagttatt gcgaatgata attaatttcg
     4861 ttaaataata ttatgatgta agagaggtag aaagtcaatg aaaaaaatag ctatctatgg
     4921 gaaaggagga attggaaaat caacaacaac ttccaattta tcggcatcac tttctcattt
     4981 aggatataag gtaatgcaga tagggtgtga cccaaaatct gattccacaa aaaacttaat
     5041 gaaaggaaaa tttataccaa cagttcttga tgtattgaaa gaaaaaaagg aagaattaaa
     5101 gcttgaagat atagtgtttg aaggttatgg aggagtatta tgtattgaag ctggagggcc
     5161 aactccaggt gtaggatgtg caggaagagg tataatagca gcttttgaaa aattggaaga
     5221 attaaaagca tttgaaatat ataatccaga tgtagtaatt tatgatgttt taggagatgt
     5281 tgtttgtggg ggcttctcaa tgccaataag aaatggattt gcaaatgaag tgtacatagt
     5341 aacttctgga gaaatgatgt caatgtacgc agcaagtaat atatctttgg ctgtagacca
     5401 atttaaaaat agaggatatg cctctttaaa agggcttata ttaaatgcaa aaaacgttga
     5461 aaatgaaagg gaattagttg aaaaattggc tgatgaaata aaaagtgaag tttttcatta
     5521 cataccaaga aataaaattg tacagcagtc tgagaataat ggacgaactg ttatagaaga
     5581 agatgaaaac aatgaaatgt cagaagtata tatagaactt gcaaataaaa ttatgaaaag
     5641 caactaaaaa agtatattta aaattaagtt tattatggag gaaattttat gaaattaaaa
     5701 aaattatgct cggtattttt acttagctca ttaatattaa caggatgctc aaaagcatca
     5761 gatggtgagg ctaaggagat aaataataat gtaaatgttg tttcagctac agtggcagct
     5821 acacaagtat tagataaatt ggatgctacg ttggtaggaa taccaactac taaatctgat
     5881 ttaccagaaa aatataaaaa cttgccagaa gtaggacagg caatgaatcc agacttagag
     5941 attgtagcat ctctaaagcc agatgtattt atagttgata gtatgtttaa agaaaatgtt
     6001 gaaaaaagca tgaaagaata taatttagat actttttatt ttgaaactgg tacatataca
     6061 caatttctta aaagtataga agatttaggt gaaaaaataa ataaacagag tgaagcaaaa
     6121 aaacttatag atgaattaga aggagtagaa caatctgtag cagaaaaagc aaaaaaacaa
     6181 gataaaaaac caaaagtagc aatcttattt ggtggtggaa ataattttat gttagctaca
     6241 aaaagttcat atcttgggga tttagtaaaa acagtaggtg ctgaaaatat aactgataac
     6301 ctaacagata atgtggaatc agattatatc caatttagtt tagaacaaat tttaaaagaa
     6361 aatccagatt atatacttag atttgcacat ggaaatattg aggagactaa aaaagcattt
     6421 gatgattctt ttgataaaaa tcctgcatgg gctacattag atgcagtaaa aaatgggaaa
     6481 gttatagatt tagattcaag tatatttaat gtatctgcaa atatacatgt taaagattca
     6541 ataaataaat tgggagatat cttgtatgga gataaataaa ttttaggtga ataatatgga
     6601 taacaaaaaa attttattga tagttacagg tttgataact ataattgttt tgctaattct
     6661 tttttctact ataggaagtg taaacttaag ccttagagaa atatttgatg cactgataaa
     6721 tgaaaataat ccgatggtta ctacgattgt atataagatg agattaccta gaaatatatt
     6781 ggcagcattg gttggagcaa acttggctgt ttctggtcta ttattacaag ctgttatgaa
     6841 aaatccatta gcagaccctg gaataacagg gatatcaact ggtgcaagtg tagctgctat
     6901 tataatattg cttctatttc cacaatacac aaatatatta ccaatagttg cctttatagg
     6961 aggtcttata tcttgtgcag tagtatattt aatggcatat aaaaatggtc taagaccaga
     7021 aaggattata ttagctgggg tagctgtaaa cactatatta ggtggagtaa tatcgtattt
     7081 atcaactatg tatagcgaaa gaattcaaag tgccatgctg tggttaaatg ggagtttggc
     7141 aacaaaaaca tggttagatg taaatatgct tatatggtat tccatagtag gtttgatagg
     7201 agcgattttc cttattagga gtgcaaatgt actacaattg ggagacgatg cagctacaaa
     7261 tctaggtttc aatgtaaata agagtagatt cataatatcg ttagtggcaa tatttttagc
     7321 tggagtttct acttctatag taggggttat tagttttgta ggtctaatcg taccacatat
     7381 aacgcgtatg ttgcttggta gtgaccataa atataccatt ccatttagta taatactagg
     7441 aagcatagtt ttattaattg cagatacatt agcgagaaca ataggaggtt ctatagagat
     7501 tccagtgggt gttattatgt ctataatagg aggtccattc ttcttatatt tattaagaaa
     7561 gagaggaagt tattaatgtt caatgtaaaa aatattaaga taggttatga agataagatt
     7621 atcatagaca acctaagtgt aagcattaaa aaaagtgaaa tagtctctat attaggacca
     7681 aatggttcag gaaaatctac acttcttaaa tcattgtcta gaattctgaa aatcaaacaa
     7741 ggtgatatat acattggtaa aaaaaatatg aaagttatga gtaataaaga gatttcaaga
     7801 caagtggcat tattggctca acacaatgta tcattaaaag atataaaggt aaaagactta
     7861 atatattatg ggagaatgcc acataaaggt ttttttgaag gtaaaaacag gaatgatgat
     7921 gagatagtga cttgggcaat gagaaataca ggtcttgaat catataagga taagttggtt
     7981 agtaatttat ctggaggtga aaggcaaaga gtgtggttag caatggctct ttgccaaaaa
     8041 ccagatatac ttttattgga tgaaccaacg acatacttag atatatcaca tcaattagaa
     8101 cttatggagc ttgtaagaga tataaataaa aaatttaaaa tgactataat tatggtttta
     8161 catgatataa atcaagctag taaatacagt gataggctta tcattatgaa taatggttct
     8221 atagtagcag atggacatcc aaataaggta ataagtgaaa aaataataaa agaagtatat
     8281 aaagtaaaat gtgatataga tatagaccca attagtaaaa agcctaggat tcatccaata
     8341 atgctatgca gaagaactag tagtatatga aagtataata ttataaataa ttaaaaagca
     8401 aaagttatta tctatgtaaa tttataagtt tgaatttata tagataatag aagtagaaag
     8461 gaaaatatat gtatacttta gatgtattaa atagattaaa agatatagat tgtgacaaag
     8521 atataaagtc tttatcatat gctgtattcc caggaaccca ttgtccattg tttggagttg
     8581 tattgactgc atcatatata agaaatatgg ctcttgtagt agtaggaaca aatgaatgta
     8641 cttactatag taaaaacttt gcatatcata ggcaatttgg caatgatagt gtttattcag
     8701 tagtactaaa agataaggac atagtttttg gtgcagaaaa aaaagtagta gaagctgtaa
     8761 aatatatttc agaaattggt gaatttgatg caataatgat agtaactaca tgtgtaccag
     8821 aattaatagg tgaagattat aatgctctgt ctagtgaatt agaagatatg cttaggatac
     8881 ctgttttgac agttaacaca gagcattata cttgcaattc tcatatactt gggatgacga
     8941 gagctttaag gagtctatca tcagttatgg aaagttacaa aaagaaagaa ggagttaata
     9001 tattaggtca tagacaagaa aatgtagagg atacagaact tgtaaaattg ctcgtaaaaa
     9061 aagatataaa aataaactgt gtaattccat ctaagtgcac tataaaagac attaaaaatg
     9121 catctagtgc taaattaaac atagtgacag atatgatagc tttggactta gcagaagata
     9181 tgaaaaataa gtttggtata gagtatgtat attttgataa aaacatgaaa gagtcaatta
     9241 taatagaaaa ttataataaa ctttgtaaaa ttttagacat agatatacaa gatgaagtat
     9301 caaagcaaag acagagatac aattcattat tgaatgaatg tagatatctg ttggatggta
     9361 aaaaattgat ttatggaaat acaccaatga tggcatttga aacagtcgat tttttaactg
     9421 aattaggact ggttccagag tttatccaag tgagagaact ttatgaacaa gataatttat
     9481 ttaaagataa tataataaaa aaaggatata atccatatgt aagtagaatt gcaaatatag
     9541 ctccacttag aagtttgtat gaaaatataa atgcagatat ttatataggg catgaaaatc
     9601 caatgttatt aagagagaaa gggcttatgc aaataacatt agatgaacat gcacaaaaaa
     9661 taggttttga gcttccaata ggaataatga aaacattaat taaattattt gatgtagaga
     9721 aatctacaag aagggaagtg atttaatatg gaaatatata gatattttcc aaatccttct
     9781 gacagaatgg gaataatatt tatagtatca tcaataaatg aagcatgtgt tatagaattt
     9841 ggaccatcag gtacaactca ttatgctata gaagcaattg gaagtttaaa tggagaagat
     9901 aaggctaaaa tttattcaac acatatgagt gaaagtgatg ttacttttgg aaattatgat
     9961 agattggaaa aagctataat tgaagtagat agtaatatta aaccaaagta tatttttgta
    10021 atggcttcat ctgtatcttc aattatagga acagatataa ttgggatatg caacatttta
    10081 aaggaatctg taaactgtag actaattcca attacaacag gtgggttgag agatgattat
    10141 aaccaaggcg tagaagagtt cctttatata cttgcaaagg aagtagtcaa agagagtagt
    10201 gaaaaatttg atagttataa tattattgga tgtactatag accagtttaa ttttttagca
    10261 gattgcgaag aaattaaaag aatgatgaaa gcttttttta aaaaagaagt aaatgtaacc
    10321 tttacatcat atacatcaat agatgaaata gaaaatgctt ctaaatcaag tctaaatata
    10381 gtacttagaa aagaaggtat aaaagcagca atttttatga aagaaaagta tagtatacct
    10441 tatgtatata agaaacctta tggaataaaa aatactgaag aatttataaa tgaaatacaa
    10501 aaagtaactg aatgggattt agatacaaat acgtatgatg atgaaatatc aaatataaaa
    10561 agatatatat ttaatgtaaa aaggaagctt tatttttatg aaggaagtaa aaaatgtgct
    10621 gtgtttggag attatgatac cgcattaggc tttagagact tattagagga gttaggtctt
    10681 aaaattgatt aaaaaatagc tacgcacact cgtgacttta gtcatga
//
LOCUS       DAMJVM010000113        10400 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.118, whole genome shotgun sequence.
ACCESSION   DAMJVM010000113 DAMJVM010000000
VERSION     DAMJVM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10400
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.118"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(347..1054)
                     /locus_tag="PC523_003058"
     CDS             complement(347..1054)
                     /locus_tag="PC523_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2476556.1"
                     /translation="MIFTKSNKYDKEFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLANEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISVDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSIKWWNELWSDSESVTVEKCEALNCHDEAWQDWLNCDNSYARNDKKMME
                     VENGKYFNTISLVARVK"
     gene            complement(1968..2837)
                     /locus_tag="PC523_003059"
     CDS             complement(1968..2837)
                     /locus_tag="PC523_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDF2476557.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITTEFKEKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKSRRISFYVLHSPLDNYSYYSTSVSFAKALGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDVKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMSMVNYFKNLGLE
                     SEFLQGKYYLEDL"
     gene            complement(3107..3577)
                     /locus_tag="PC523_003060"
     CDS             complement(3107..3577)
                     /locus_tag="PC523_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HDF2476558.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYQELFEQEIELDLGKNVTFRGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDNFDKFIEKLNSYKEVEFVHKPLKYEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLAEGYSAEETSKIIQHPIEFVKQCENSKQ"
     gene            complement(4071..4460)
                     /locus_tag="PC523_003061"
     CDS             complement(4071..4460)
                     /locus_tag="PC523_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2476559.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYKWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEEDV"
     gene            complement(5543..6949)
                     /locus_tag="PC523_003062"
     CDS             complement(5543..6949)
                     /locus_tag="PC523_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2476560.1"
                     /translation="MDSVKYILKRFIGSSILISLLFIILNIAGFLLFSYMIFVSKYSV
                     DLDTSDSREIKSNLIMLEENLILKDGKFILNDSSKEVLHKNKIWAMLINNNGDRVWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEYPNGILVTGYPKDKYTKFNLTYTIKE
                     IKQFPMLIICMFLVNAIVIFLIALFIGLKMVKSIKPIINGIKLISNGEPVILKEKGIL
                     SDISKSINTVSKELQMKDEKLRNKDEARSNWIAGISHDIRTPLSMIIGYAGELEESRN
                     LSNKEQEQVSIICNQGVKIRELVNDLNLVSKLEYNMQVLNCDKIRVSTFLRELVSEFI
                     NDNLDNDFYIELDILNEDIIVDADKKLLKRAISNLIQNSITHNQQGCNIKVISKSDLD
                     FCYIIVEDDGKGMSQEKINSLLKSQNGLSEAYAKGQNHGLGILIVAGIINAHGGKLDI
                     TSIDGKGLKTILKLPIIK"
     gene            complement(6994..7701)
                     /locus_tag="PC523_003063"
     CDS             complement(6994..7701)
                     /locus_tag="PC523_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2476561.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRIFLASSCEEAV
                     RVFRNIKPDCAILDVVLPDGDGFSIMRKIRETSKMPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVSILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTSDSLCQAIWGDDMYGYENTLMVHIRRVREKIERTPST
                     PKYLLTVRGLGYKLMIN"
     gene            7862..8785
                     /locus_tag="PC523_003064"
     CDS             7862..8785
                     /locus_tag="PC523_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2476562.1"
                     /translation="MKHMEMIIRTQRLSKEYNKIFRVKDIDLRVPKGAVYGFLGPNGA
                     GKSTTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENM
                     IIMQRLLDLPKKNINEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKLPKLLILD
                     EPTNGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQD
                     IIKELHNRGQSQIAIKTNNIVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINNML
                     VHSNIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            8785..9546
                     /locus_tag="PC523_003065"
     CDS             8785..9546
                     /locus_tag="PC523_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2476563.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIINSNRAISRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVASLNGIYL
                     SKFLCSAILVSVPVLIQVLSIVLFGYFRGVIESLSVFLLLKYILGTVFVNIAIIALHL
                     WIAIVIPNQMVAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDTNFSITILVFLIGILIYYIGRNHLLKKEV"
     gene            9558..10295
                     /locus_tag="PC523_003066"
     CDS             9558..10295
                     /locus_tag="PC523_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2476564.1"
                     /translation="MLRKSVFGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSMLTAITQVFFVVFYIVAGNMFGFSGNIPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVANPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     AVCVSFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         3545 a         1635 c         1113 g         4107 t
ORIGIN      
        1 tttaaagtat aataaaatat ataaaaaaac atctaaaatg gtactataaa atctttaatc
       61 ttcatcaata tcttttttgc cttttattgt tataactgct tttttattaa ttttttctca
      121 taaaataaac atttagtatt aaaatatata tagcgcaata gtatttgaca tctaacctta
      181 gaaaaaataa aaatgcccaa ttgcactaaa aaattcgatt gggcataaat aatataaaaa
      241 atcatttttt atataaactc ttcttttaac ttacgttttt atttaacttc tgctttcatt
      301 caacttatat ttctattcgt ttaatactct cacttatctt atacttttat ttaactcttg
      361 ctaccaagga aattgtatta aaatactttc cattctctac ttccatcatt tttttgtcat
      421 ttctagcgta cgaattatcg cagtttagcc aatcctgcca agcttcatca tgacaattta
      481 aagcttcaca tttctctacc gtcacagact cagagtcaga ccaaagctca ttccaccatt
      541 taattgaatg gaagttcatg ttgtcttgcc aaaatggaat gagttcatct ggtatacaat
      601 ccacaaagtc ttcttttaat ccaggcattg ccatagcaag tattcccccc tcttttacaa
      661 gaggtgatat atgattatcc aaaaaacctt ctttgtttcc aaaatagtga taagaatcta
      721 cacttatcac tgcatcaaag aaaccttctg cataaggtaa ttcatgtgcc tctgcctgta
      781 ttggaaatat tttgtcatcc aatttaaact ctttaaatct tttatagttt tcagttggtt
      841 ctatccataa atctgttgca aatacagtag catcaaattc atttgctaga aatatcgagg
      901 atataccttt cccacatcct aaatctaaaa tacgcatccc cttttctagt tttatcttac
      961 ttgttaattc ttccaatatc tttatacaat taggtcccat catatttttc ataagaaatt
     1021 ccttatcgta tttattactt tttgtaaata tcattctatc tttcctttcc ctatcaatta
     1081 ttatttatta aattttatac gtttgatttt ttatttttta tatgcttctt ataatgagca
     1141 atctcttttt tattcattct ttaacaggta attttaatga gtaattttat ccactctaca
     1201 ataatcactt taatgagtaa ttttatccac tctattatag ttattttaat gggtaatttt
     1261 atccactcta caataattac tttaatgagt aattttatcc actctattat agttatttta
     1321 atgggtaatt ttatctactc tacaataatc accttaatga gtaattttat ccactctatt
     1381 atagttattt taatgggtaa ttttatctac tctacaataa tcaccttaat gagtaatctt
     1441 atccactcta ttatagttat tttaatgggt aattttatct actctataat agtcacttta
     1501 ataagtaatc ataattttta tatcatttta gatacttact ctaataagaa gtcgttttct
     1561 gatacttgat aagttttaat tacttccact ttaacctata tactaattaa cttatttaat
     1621 ttagtgtata tttgacactt taccttttat tataccattt attctcataa atataataat
     1681 tctatttttt taaaataaat atttataata atctaaaata aatatttata aattctaatc
     1741 aatcataaat aataatctaa aatatatcaa aaaataaact taaaaacctc gaaaaagaac
     1801 ttaaaagaca tcaaaactaa agcttctaat cagcaattaa aagtctattt tcttagttaa
     1861 aaaaataaag atagattttt tcaaaacatt ggataaatgg gtaaaaaaaa taaacagtca
     1921 gtaaaacaaa tttttaccaa ctgtttctct ttattattta atatatccta taaatcctcc
     1981 aagtaatact ttccttgtaa aaattctgac tctaaaccta gatttttaaa ataattgacc
     2041 atgctcatac atgcaaattt ttctgtagaa tagtgagttg cacctataac attaattaaa
     2101 ttatcttttg ctatttgatg aaactctaac actggttcaa aatgttttaa aggtttagta
     2161 aatccagtaa tatataggtt tataccaagt tcagcaactt ctcttgctac aaatggataa
     2221 cttcctcccc ctgctgcaat agcaactaaa ccatctttta atacagaatc tccataatca
     2281 tacaatttaa catcatgacc aacagatttt ttaattaatt ccttaatctc ttctattgat
     2341 tttagtgatg tcttacatat aactcctacc tcaattttat cgtcatattt gcaaaatggt
     2401 ttgactattt ctaaacctag ggcttttgca aaactaacag aagttgaata gtaactatag
     2461 ttgtctagtg gagaatgtaa tacataaaaa gaaattctac gacttttcat ctcttccata
     2521 taagatagtg gtatgtcgtg aaatggaaaa ccttcatctg atgctatgta tcccattgca
     2581 tgatgtgaaa aaattaaaat atctttttca tctttatcta taacctgttt aattatctct
     2641 ttatcaggaa aagtcgttgt gtatacttta ttaatatttt gagcattatc taatactaat
     2701 cctatgtact tttctttaaa ttctgtagtg ataaaatcat tttcgaaatt cataaaactc
     2761 caatcatcat taatattttt aatattaaat tctaagtcta attttttata aagttctatt
     2821 gccttgataa taaccactcc tttataacaa caattgatta atacattact aatataggaa
     2881 tttcaggata ataaacttta tatatttaag tatttctact tcaaatttaa aatattataa
     2941 atattcaaat ttattaatat attttaatat cacatttatt tttcctttat taatcttttc
     3001 tctattttaa tttaatttat aacattagtc aaatactaat cctatatatt atcttaaatt
     3061 agttccacta caattaattt ctactaaata attataattt aaacaatcat tgcttactat
     3121 tttcacactg ctttacaaat tcaattggat gttgaattat tttagaagtt tcttctgcag
     3181 agtatccctc agctaaatac cttctagcta taacttccat agactctcct atctcaataa
     3241 tatgtttatc tgggtcataa atacgcacta ccctttgttg ccattcatat tttagtggct
     3301 tatgaacaaa ctctacttct ttgtaagaat tgagtttttc aataaatttg tcaaaattat
     3361 caacctcaaa ataaagttcc atattatttg atttttgtat aattgtatct ggattaatcc
     3421 ctgtaagcca tgcgaagtct tcttgtatgg caaatcctcc tctaaatgtt acatttttac
     3481 ctaagtccaa ctcaatctct tgctcaaata attcttggta aaattttttt gatacttcga
     3541 tatccttcac tgctaataat gaaacctgat atttcataaa ttttcctcct aattgtttat
     3601 ttgttcttta tttctatttt tttcaatatc ttctattaac acttttacct atgtaacctc
     3661 aatcccttct attaatcctt catttttact tttcagtatc ttttattaag cccttatttt
     3721 tactttttca acatcttata ttaacacttc acttctgtct cttcaatatc ttcattaata
     3781 gctatttatt ttatcatctt aagcttttac agtttatttt taaacttcta tttaaatctc
     3841 attgtcttca ttaaaaaagc tacctagtac ttatataatt ataggtagct tttttaaatt
     3901 taattaaata gaatatccat tactaattta tgtttacatt taacctgttt tagattcaca
     3961 ttataatgtt ataactcact tataataagt tattatatta cttaagtaga ctaattatat
     4021 aaatatatct acacacgatt tatattgatg ctatacaaaa atacgtttgc ctatacatct
     4081 tcctcctctt cttttagatt ttttatccac tcttcaataa gtaagatatc atctttgctc
     4141 agattaatat tttcatgtaa tgataataat aaacttttta tagaagaatc atgaatattt
     4201 ctaaaaaaat ctctagtttc aactcctatg tattcctttt ctttaattaa aacttcataa
     4261 tgtgtatatt tatctatctt ttgagaattt agaaatcttt ttactactaa tctagataaa
     4321 agagtcaaag ttgtcgtttg tttccattta tatttttgct ccattcctaa aacaatatct
     4381 ctagatgtta ctttggaatc tgatttccat ataaatttca ttatctttaa ttctgattgt
     4441 ggcaactttt ttatagtcat gataaaacct tgctactcag aatatagaca agcaatatcc
     4501 ataataaaat tcttcgatta aatacatctt atacggattt atataagctc agatttataa
     4561 gatatttttc aatcttaatc ctaaatttag ctatttgtac atggtaaata tatataaata
     4621 atgtcaaaat tttcgtatta tattgcttaa ttttatgaaa tattttgtac tcagctatct
     4681 attttggata cttgtccagt agctctcctt tcttttaaat tatttgattt taaatatttg
     4741 tattccagtg aatcctactt cctaaatttc ttacttaata caacttatta ctaaacttcc
     4801 tacttcctaa atttcttact tcttaagaat agtgttaatt gtaattttaa gattataatt
     4861 tttgccttgt ttaattgaaa cattctatat agttatatcc ttctatttta gagttttttt
     4921 atttatatct tacaaagttt aaattttttc attaattata ttataaatat tctatatatt
     4981 ttcatttaaa tatatatata tatatatgtt atttctaaaa atactacaca aaactctact
     5041 ttagtagaac ttaaagacgc aaaatacttc aatttaaaac atttatcgta ctttaaataa
     5101 ataactataa taaaatatac atacattagt tacataaaag ctattataac tacacgtaac
     5161 agcgtacaat taaaactttt ttagcatgtc attatatttt ttcttaaaat taaaaaacac
     5221 tatatcataa ttctatttta gtagaatttt tttgtcaata ataaaattaa atattaatta
     5281 aaaaataaaa aaaatggaat tatctaaaga atttaataac cttacaataa ttccaaaata
     5341 ttatttctat gaaaattttg ataccatgag taacttagct ataagcgcat aaagcacaag
     5401 ttactcttta gtttaataaa acttgacaag aaaagaattt atccttattc tactctaata
     5461 gagttacttt gtcaataaag aataaaatta ttttcaaaat atttccattc ttcattttta
     5521 tctgtcatta ttttataaaa tatcacttaa ttataggtaa ttttagtatt gtttttagtc
     5581 ccttaccatc aatactcgta atatccaatt tgcctccatg tgcattaata ataccagcca
     5641 ctatcaaaat acctagacca tgattttgac ctttagcata tgcctcactt agtccatttt
     5701 gactctttaa taaactatta attttttctt gactcatacc ttttccatca tcttcgacaa
     5761 ttatatagca aaaatctaaa tcagacttag atataacctt tatgttacac ccttgttggt
     5821 tgtgagttat actattttgt attaaattac taatagccct ttttaataat tttttatctg
     5881 catctacaat aatatcttca tttagtatat caagttcaat ataaaagtca ttatccagat
     5941 tatcatttat aaactcagaa acaagttctc tcaaaaacgt agaaactcta atcttatcac
     6001 aattcaatac ttgcatatta tattcaagtt tagacactaa attgaggtca tttactaatt
     6061 ctctaatttt aaccccttga ttgcatataa tagacacttg ctcttgctct ttatttgaaa
     6121 gattacggct ttcttcaagt tctccagcat atccaattat cattgaaagt ggtgttctta
     6181 tatcatgaga tattcctgca atccagttgg acctagcttc atctttgttc ctaagctttt
     6241 catctttcat ttgcaattct ttagatacag tattaataga ttttgaaatg tcactcaata
     6301 ttcccttttc tttgagaatt actggttcgc catttgatat aagctttatc ccattaataa
     6361 ttggttttat agatttcacc atttttaagc ctatgaatag agctattaaa aaaataacta
     6421 tcgcattaac cagaaacata catattatta acataggaaa ttgctttatt tccttaatcg
     6481 tataagtaag gttaaactta gtgtacttgt cctttggata acctgttact aatattccat
     6541 ttggatattc ccatacatac acaggataat cctttaaaaa gaaccttgaa aactttgcta
     6601 catctgtcaa agtataatga cttggaattt cttttggtaa atcaaatccc catactctat
     6661 ctccattgtt attaattaac attgcccaaa ttttattttt atgtagtact tccttactag
     6721 aatcgtttaa aataaacttt ccatctttta aaatcaaatt ttcttcaagc ataatcaagt
     6781 tggattttat ttccctacta tcacttgtat ctaaatcaac agagtatttg gatacaaata
     6841 tcatatatga aaacagaaga aaacctgcta tatttaatat aataaaaagt aatgaaatca
     6901 ggatacttga acctataaat cgcttcaata tatactttac actatccaaa attaaatcac
     6961 tcctcttgat ttcttttatc ctaatagcta aaattaatta atcatcaact tatatccaag
     7021 acctcttact gtaagaagat atttaggtgt agatggagtt ctttctatct tctctctaac
     7081 ccttctaata tgaaccataa gggtattttc atatccatac atatcatccc cccaaatagc
     7141 ttgacatagt gaatcactcg taacaattct acctttattt tcatacaact ttaaaagcag
     7201 tgaatgctct ttagctgtta gagaaatctc ttgcccatct ttctcaacac atgcactatt
     7261 taaatttaca acaacagaat ctcccagttt aaatactggt aactcttctt cttctatagg
     7321 cacatataca cgatttaata tgctcacaag tcttaaagta agttcctttg gtaaaaatgg
     7381 ctttactata taatcatcag ctcctaagcc caaaccaatc aatctatcct catcttcgcc
     7441 tcttgcagaa agaaaaataa ctggcatttt agatgtctct ctaatttttc tcataataga
     7501 gaaaccatct ccatctggca atacaacatc taaaattgca caatctggct ttatatttct
     7561 aaatacccta acagcttctt cacaacttga tgctaaaaat attctactaa atccctcttt
     7621 tcttaggatt tcatctatca tttttaataa ttccttgtgg tcatcaacca caagtatttt
     7681 tttatctttt attgactcca caatcatcac cctcctttag tataccatat accattttca
     7741 cagctttgcc taaatagact atttaaggct attgtaaggt aaacttcagc taattgaaat
     7801 gtagttcatt tattatactt atataagcta aaaaaatcaa taaacacaga ttaaaaaggg
     7861 gatgaaacat atggaaatga taattagaac acaaagactt tcaaaagagt acaataaaat
     7921 atttagagta aaggatatag acctaagagt tccaaaagga gctgtatacg gttttttagg
     7981 tcctaatgga gccggaaaat ctaccactct taaaatgctt cttggacttg ctaaacccac
     8041 acaaggaaat attaatattt taggtaaaga attaaatgaa aaaaatagaa tatcaatttt
     8101 aaaagatata ggttctttaa tagaatctcc atcatactat ggtcacctga cagggcttga
     8161 aaacatgatt ataatgcaaa gactcttaga tttgcctaaa aaaaatataa atgaagcatt
     8221 aaaaattgta aggttagaaa atcaaaaaaa taaaaaagtt agtcaatatt ctttaggtat
     8281 gaagcaacgt cttggcattg ccatggctat aatgaaactt cctaagttat taatacttga
     8341 tgaacctaca aatggacttg acccagcagg tattgaggaa attagagaat taataaaatc
     8401 tctaccagaa aaatacggta tgacagttct aatctctagc catcttttaa gtgaaattga
     8461 ccaaattgcc acatcaatag gaataataaa tcatggcgaa ctggtttttc aagatatcat
     8521 aaaagaactt cataatagag gtcaaagtca gattgctatc aaaactaata atattgtatc
     8581 tgcacaaaag ctattgaata ctaaaggata tatgtcactt atacaagatg gatatttaac
     8641 tattgaaaat ttaaaagact cagatgttgc taaaataaac aatatgcttg tccattcaaa
     8701 tatagatgta ttcagaatag aagaacataa aaagagcttg gaagatatct tcctagaact
     8761 tactggtaag gcggtgactt tataatgaaa taccttggtc tagaatttta taaacttaaa
     8821 agaaagaaga ttattctgat gaccttttta tttgtagctg ttgaaataat gtggtgcatc
     8881 ataaattcaa atagagctat ttcacgtaat ccagatatgt taggtggatt tgaatattca
     8941 tatatgctta tgagttttgc aagtttaaat ggtctattct ttcctatctt aatatcaata
     9001 attacatcac gtataagcga tatagaacat aaaggagata catggaagct tttaaaatct
     9061 tctgtggcat ccttaaatgg tatttattta tctaaatttt tatgctctgc tattttggta
     9121 tctgtacctg tattgataca agtgttgtct attgtgctat ttggatattt tagaggagta
     9181 atcgagtcac tttctgtctt tttactttta aaatatatat taggaacagt ttttgtaaat
     9241 attgctatca ttgcactgca cctatggatt gcaattgtaa ttccaaatca aatggttgca
     9301 attacatttg gtatgattgg cagttttata ggtttgacat caggattgtt ttatcaaggt
     9361 attcgtagat tgtttatatg gtcttattac ctagagttat cacctttaag ttacgcttat
     9421 gataatgttt taggttcatc tgtttataga attgatacga atttttctat tacgatttta
     9481 gttttcttaa ttggaatatt aatttattat attggaagaa atcatttatt aaaaaaagaa
     9541 gtataggagg gatttaaatg ttaaggaaat cagtttttgg agaaattcta aagttaaaaa
     9601 attcttttat atggtatgta ttattagctt tgccacttat aagtgtattg attggctctg
     9661 gtaattttta tctaaatcaa ggcatactca aaaaagaatg gtacagctta tggactcaag
     9721 ttagtctttt ttatggggaa tttttctttc ctatcttaat cgcaatcttt tgtgcatata
     9781 tttgtaggct tgaacatatg aatcataatt ggaataatgt aatgacattg ccagttaatt
     9841 taaaaaatat ctttttatct aaacttacag tagtttcaat gcttacagct attacacaag
     9901 ttttctttgt tgtcttttat attgttgcag gaaatatgtt tggtttttct ggtaatatac
     9961 ctaaagaatt gactggttgg attgctaaag gttggattat ttccatttca atagcttcta
    10021 tacagttata catgtctata aagattagaa gttttgcaac acctattggt attagtcttt
    10081 gcctttcttt atttggtatg ggattaatag tagcaaatcc tgctgttggt atatttttcc
    10141 cttattctat gttaggtagt gctatggggg ttataaagca atctgcttta aatgttaatg
    10201 acactatagt tgcagtatgt gtttcattta ttactactat agtattttct gaaattgcca
    10261 atagaaattt tagaaagaat gatgttattt cttaatggta aacgttagat atttttttgt
    10321 ttaaaattta tattcttttt agttgaaata tatatccctt ttagttaaaa atctatatct
    10381 tttattttaa ttttatatgt
//
LOCUS       DAMJVM010000114        10303 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.119, whole genome shotgun sequence.
ACCESSION   DAMJVM010000114 DAMJVM010000000
VERSION     DAMJVM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10303
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.119"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(157..300)
                     /gene="scfA"
                     /locus_tag="PC523_003067"
     CDS             complement(157..300)
                     /gene="scfA"
                     /locus_tag="PC523_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HDF2476565.1"
                     /translation="MKNKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(366..728)
                     /locus_tag="PC523_003068"
     CDS             complement(366..728)
                     /locus_tag="PC523_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HDF2476566.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(786..1079)
                     /gene="yajC"
                     /locus_tag="PC523_003069"
     CDS             complement(786..1079)
                     /gene="yajC"
                     /locus_tag="PC523_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDF2476567.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLGV
                     GDKVVTIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(1213..2334)
                     /gene="tgt"
                     /locus_tag="PC523_003070"
     CDS             complement(1213..2334)
                     /gene="tgt"
                     /locus_tag="PC523_003070"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDF2476568.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHNFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPADREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITSIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(2413..2898)
                     /locus_tag="PC523_003071"
     CDS             complement(2413..2898)
                     /locus_tag="PC523_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476569.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKAQQNKEDEDKS
                     I"
     gene            complement(2910..3935)
                     /gene="queA"
                     /locus_tag="PC523_003072"
     CDS             complement(2910..3935)
                     /gene="queA"
                     /locus_tag="PC523_003072"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDF2476570.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLMGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLSKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGNNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(4253..5272)
                     /gene="ruvB"
                     /locus_tag="PC523_003073"
     CDS             complement(4253..5272)
                     /gene="ruvB"
                     /locus_tag="PC523_003073"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDF2476571.1"
                     /translation="MQGFEDENRIITSTMKVEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSNILDAKIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(5379..5981)
                     /gene="ruvA"
                     /locus_tag="PC523_003074"
     CDS             complement(5379..5981)
                     /gene="ruvA"
                     /locus_tag="PC523_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDF2476572.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLAESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(5974..6483)
                     /gene="ruvC"
                     /locus_tag="PC523_003075"
     CDS             complement(5974..6483)
                     /gene="ruvC"
                     /locus_tag="PC523_003075"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDF2476573.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(6795..7424)
                     /locus_tag="PC523_003076"
     CDS             complement(6795..7424)
                     /locus_tag="PC523_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476574.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDTGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            7627..7950
                     /locus_tag="PC523_003077"
     CDS             7627..7950
                     /locus_tag="PC523_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HDF2476575.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            8214..9068
                     /locus_tag="PC523_003078"
     CDS             8214..9068
                     /locus_tag="PC523_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476576.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEVYFILDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKFITQETIENKLESLVENRLDTLEDRLE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(9254..10255)
                     /locus_tag="PC523_003079"
                     /pseudo
     CDS             complement(9254..10255)
                     /locus_tag="PC523_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
BASE COUNT         3296 a         1836 c         1162 g         4009 t
ORIGIN      
        1 gatatttttg cataattaaa cattaagaaa tcaaatttaa ttttatataa atagtttaaa
       61 tattttatat acaaacaaaa ataaaagagg atacctccta atgataaaag aatcacattg
      121 agataccctc ttacaaacaa ctaaatcaat aattttctat ctttcacact cttgatttgc
      181 aactgtacaa gaagttttac aagctgattg acaagaagtt tgacactctc cacatccacc
      241 ttttgcagca ctttgcttta aagtagcttg agataaagtt tttacatgtt tatttttcat
      301 tttaaacgcc tccatttcta attgtttcct attgattata tcataaaact accttaatat
      361 aaactctatt taaagttgac acccaatacg cctccaatgc ctcctgctgc tgatattagc
      421 aataacttat aaatcattcc tacttcgaat acaaactttt cttgtgctaa aaacacagtc
      481 aaaaaaatac atactatgta caaaagtcct actaaaagcc catacataag tcctttttct
      541 ttcatgtgtt ttgaagtata aaaccctcct atagctgcag aaatagtagt tataaatgat
      601 gaaaccatag ttatattatc cccaccaata tctgtaaatg tcaaaaataa gttatacact
      661 aataaaacag ccaaagttat tatatatgca tatcctagac ctttaaaaat ataattagat
      721 tttcccataa aaaaacctcc tctcaaatta atatatattc gagaaaaggc ttttaaatta
      781 ctaatttagt cttcttctat ttcttctttt ttagatttaa cagttcctat agcccatttt
      841 tcaaatggta ctttagttct atttggtcct aattctaata ttacaacatc atcttcaact
      901 ttagctacat tagctactat tccacctatt gtaacgacct tgtctcctac acctaggcta
      961 cttctcattt cttttaattg tttgtctttt ttcttttgag gtctaatcat taggaaataa
     1021 aagattgcga ttactactac ccatagacct atactcataa ttatttgttg ctgtggcatt
     1081 aatttttcac tcctttgtaa attatactct tttgtaaatc ataataaatg tttatataac
     1141 aatttacttt ctctatcttt gagtactatt gaatattata tattaaaatg attataatta
     1201 atagtgcaaa atctatattt catatccata ttttgcaaaa aattcatttc taaaatcaag
     1261 taatctatct tccattatcg cctgtcttat ttgtttcatt aaatttaata agaagtgaag
     1321 gttgtgagta gttattagtc ttgcacccag tatttcattt gcttttataa ggtgtcttat
     1381 ataagctctt gaatagtttt tacaagcata acaatcacat tcagggtcaa gtggagtaaa
     1441 gtcttcagca taagttgcat ttctaactac tactttacct tggctagtca ttgcagttcc
     1501 atttctagcg atacgagtag gtaagacgca atcaaacatg tctactcctc ttataacacc
     1561 ttccactaaa tcatctggac taccaactcc cattaggtac cttggtttat cctttggcat
     1621 aagtggagtt gtatgctcca gcacatcata cataagtggt tttggctctc caacactaag
     1681 cccaccaata gcatatcctg gtaagtcaat actagttatc tcttttgctg attgctctct
     1741 taaatctttg tacatcccac cttgtattat accaaataaa gcttgcttat ctgtattatt
     1801 atgggcatct ttacatcttt ttaaccatct tgttgttctt tctaaagaat tttttacata
     1861 ttctctatct gctgggtatg gtgcacactc atcaaacgcc atcattatat ctgaacccaa
     1921 cgcattttgt atttccatag ctttttctgg agttagaaaa tgttttgaac catctaagtg
     1981 tgacctaaat tccacacctt cttcttttat ctttcttaaa ggacctaagc taaacacttg
     2041 gaatcctcca ctatctgtaa gtattggttt atcccaattc ataaaattat gaagaccacc
     2101 agctcttttt ataagttcat gacctggtct catatataaa tggtatgtgt tactaagtat
     2161 tatttgtgaa ccaatctctt ttaattcttc tggtgtcata gattttacag ttgcttgagt
     2221 tccaactggc atgaatattg gcgtttcaat aattccatgt ggagtgtgta atcttcctaa
     2281 ccttgcgcca ctctgcttac aagtctttat caattcgtat cttactgcgt acataaagtt
     2341 ctccttaatc atatttattt ctcattattt cttaagatat atttgtaata agctattcat
     2401 ataatttatt gtctaaatag atttatcttc atcttctttg ttctgttgcg ccttaattgc
     2461 ttcttcatca tgaatactca ttatattaag agcactcttg tcttttgtta ctaaaaactt
     2521 ttttatagca aacagccctg caatagctat aactcctatt aaaacatcta tcataccttc
     2581 atttttaggc agtaacaaca tttttctagc tatagcgtaa agtaatgcct caataaatgc
     2641 tcctggtata tgaagagata acattactac aagttcaaca cctatcacta agagtaatat
     2701 ttgtgctaaa aaatcattaa gctgactgta ttgtactgga ttttgaaaat caactatata
     2761 cgctccccac ataagtctta gaacatctat agttcctaaa aatactgcta ttagaactac
     2821 tactgctaaa acacgttcaa atataaaagc aatctttaat atagggttat tctttttttg
     2881 tttttgtaaa ctatgcatca attacctccc tactttataa tcatagcatc tccaaaactg
     2941 aagaatctat atctttcttt tactgcctca ttatatgcat ttaaaacatt atcctttcca
     3001 caaatagcac ttactaacat tattagtgta gactctggca aatgaaaatt agtaataagt
     3061 ttatctaaaa ctttaaattt ataacctggg tatataaata tttctgtcca tccactggtt
     3121 tctttcattt ttccatcctc attacaagct gattctatag ttctacaact agtagtacca
     3181 acacatatta cattatttcc attttctttt gctctattta ttttgtcagc tgcttcttgg
     3241 ctaaccatat aatattctga atgcatcttg tgatttaaaa catcttctac ttttactggt
     3301 ctaaaagtgc ctaaaccaac atgtagagtt acaaaagcta tatctacacc tttttctttt
     3361 attttactta acaattcttc tgtaaagtgt aatcctgctg taggtgctgc tgctgaacca
     3421 ttatgctttg aataaacagt ctgatatctt tctttttcat ctaatctagc agtgatatat
     3481 ggaggtagtg gcatatttcc aagttcatcc aagatttctt caaaaattcc atcataatga
     3541 aattttataa ttcttgaacc ttcatctgat aaatctacaa cttctccaat taactttcca
     3601 tttccaaaag aaaatttagt ccctactttt gctctttttc ctggttttac taaagcctgc
     3661 caagtatctt cctctgttct ttttaataat aaaaactcaa ttttacctcc agtttcaagt
     3721 ttctctccca tcaatctggc tggtataact ctagtattat ttaatactaa acaatcccct
     3781 ggatttaaat attctattat atctctaaat actttatgct caatattgcc tgtctcttta
     3841 tctaatacca tcaatcttga actagctcta tcttctatag gaacttgagc tataagttcc
     3901 tgtggtaagt caaatttaaa atcacttgtt ttcaatgtta aacttcctct cttaaaattt
     3961 ctccagctac aataatatca aaaaaataac ttattttcca caatattacc ataatctatc
     4021 aagaaattgc tatttttaac agacatcata tccacaatat atagatattt ttccacgata
     4081 tgcacgtatt atccacaata tatagataca atacgcacaa attttatcgt atttatccac
     4141 agtatctaca gatataatta ccattttgtg gatatatttg aatttttagc cattttagaa
     4201 atattttaat ttaatgacta ttctatttta taaatacttg cataatctat ttttattttt
     4261 cattaggata tggaattttt aagtgttcat atgctaatgg catggctacc cttcctctag
     4321 gtcctctatt tataaatcct aattgaagca aatatggctc atatacatct tctattgtat
     4381 ttctatcttc tccaatagaa gctgctaatg tatccagacc tactggacct cctctaaatt
     4441 tttctattat agtcataaga agcttctcat ctacaaaatc aagacccaaa ctatcaactc
     4501 ctaaaagttc taaagcatct tttgccacct tatcagttat ttttccatca gctctaactt
     4561 gtgcaaaatc tctaactctt tttagtaatc tatttgctat ccttggagtc cctcttgaac
     4621 gtttagcaag ttctaacgct ccattagact gtatctttgc atctaaaata tttgatgacc
     4681 taagtactat ttttgaaagc tcatcaactg tataataatc cagtttacaa ataacaccaa
     4741 atctatctct aagtggattt gtaagcatac cagctcttgt agtagctcca ataagtgtaa
     4801 atttaggaag gtcaagtctt atgcttcttg cagatggccc ttttcctatt attatatcta
     4861 ggcaaaaatc ctccattgct ggatataaaa cttcttcaac acttctattg attctatgta
     4921 tctcatctat aaataaaaca tcattttcat ttaaatttgt aagtatggct gccaaatctc
     4981 cagctctttc tattgcaggt cctgacgtaa ttcttaaatt tacccccatc tcattggcta
     5041 ttatacttgc tagtgtagtt tttccaagac ctggtggtcc atataaaagc acatggtcta
     5101 attgctcatt tctcgattta gctgcttcta taaatatact aagttgctct ttggattttt
     5161 cttgccctaa ataatcttct aaagtttttg gtcttaaact gttttctata tctatatctt
     5221 ccaccttcat tgtagaagtt attatcctat tttcatcttc aaaaccttgc atttataccc
     5281 ctcctttcgt acttttgaaa atctacttta tatattttca aatttacata aactccttaa
     5341 atattgtttt aaaatctaaa tattataaaa tccaaatatt attttaagga tttactcatt
     5401 atatatgtaa gtgctttctt tataatagct tctgtattca taccatcttt tttacatttt
     5461 tgaactgcct cttttgattc cgccaaagta tagcctagag ctacaagtgc atctacagac
     5521 tcatcttgag atatcaaggt aggtttttgt gataataatg ttggttcaaa ctctatgtta
     5581 tttttatcta ccttatcctt aagttctaag acaattcttt ctgctgtctt cttaccaaca
     5641 ccaggagctt tggataactt tccaatatct tcacttaaaa tatatgcgcc taattgtgct
     5701 ggtgatgcaa atgatagtat tgaaagtgct accttaggtc ctatttttga cacagatgta
     5761 agtagttcaa acatcttaag ttcttctcta cttacaaaac cacaaagact catatcatct
     5821 tctcttacaa ttaatttagt atatattttt gtggcttctc ccacttgtaa attcattatt
     5881 gtattggatg atacatttat tttatatcct atcccattat tttcaacaac tatactgtct
     5941 atataaattt cttctactgt accttttata taactataca tatttaccac ctatattctt
     6001 tagagttttt tcaagtttgt ttgagtgagc atggcaaata gccacagcta aagcatctgc
     6061 aacatcatct ggttttggaa ctttttttag acttaaaaat gaagttacca tctgttgaac
     6121 ttgtgcttta tctgctctcc catatccaac tacaccttgt tttacttgaa gtggagtgta
     6181 ttcatataca ggcttcccat tatgagcaca tgcaagcata gtaacacctc tagcttgtgc
     6241 aactgttata gctgttttta cattcttgtt aaagaataat tcttccattc caacttcatc
     6301 tatattgtaa ttctttacta ttgtatcaat tcctttatat acaagttcta atcttctcga
     6361 tatattcata tgggcaggtg ttgtaactgc tccataatcg attactttaa acttgctatt
     6421 tttgtattca attataccat atccaactat agctatacct gggtcaatcc ccaatattat
     6481 cattttttaa aattccccct aaaaataaaa gaaataccaa aatgtatttc tcataaaaac
     6541 ttatgttcta aatcattata tcataaataa tattaacaaa tcataaaatg ctctatgtaa
     6601 aatataaaat caaataaaaa agttatatca aagcaagatt ttacctcaat ataactttta
     6661 tatttagtac aaataaatta ttgcattttt atatgaatta aatgtgacta caattttaat
     6721 actaatctat ctaatattaa attatttatt attaacttac ttagtattta acttgaaata
     6781 gtattaattt actattaact tgcaaagtct tctaatcttg aataggcctc ttcttcagta
     6841 tccataacta cacctttttt gatttcatct tgaactgttt taggaagtga atctactctt
     6901 atttttgttt tttctactaa tgttttactt ccagtatcat catatttata tagtgctata
     6961 tttccattat cattttcaat agtatattta ccttttatag taacatccat tgcttgtaca
     7021 tctttattat cactatattt agattctacc aatatgattt catttttatc catactcttt
     7081 atttttttag ttggatattc ttttttaaaa tatgatacta tttctgactt tgtcttatct
     7141 aataatgact ctggaactat tcctgccatt gtaagtgttc tcttatagtt ggtttcattt
     7201 tcgccttcac tttgaagcca aacttcacaa tttttattta atccaaaagc ttcttttgac
     7261 ttttcattca tattcgcact agtacttaca acttcaccag tttgaactcc attgtgtttt
     7321 aatcttgtcc ctatataaac tccactactt aatacaaata ctccaactag tattaaggca
     7381 gatattttcc aaggaaataa ttcttttttc ttcttttcaa acattgatta cgcctccttt
     7441 ttctattatt ttctccaagc tgtaagtttt tatacaattt aggtaaattt tagaagaaaa
     7501 tatatttata tttcaaatta ctctaagaat agatattttc taaatggtag ttttctctta
     7561 atagttatat tcttgtaatt atatggacat aatttataat aaataatcgt ttaggaggta
     7621 ataactttgg aaagaataaa ctgtaatgta attaactgtt cacataacaa cagtcatata
     7681 tgttatgcag ataaaatttt tataaatggt gaaaaagctc acaatgacag acatacttgt
     7741 tgtagttcat ttttagatga atctatttat agcaatctat ctagcaatgt taataataga
     7801 aattgtgagg aattagtttg tactgtaaaa aattgtaaat acaataaaga tggtagttta
     7861 tgtacacttg ataatatatt ggttgctcct gaaactgata gagtgaacct ttatattgaa
     7921 acatgttgct ctagtttcga atgtaaatag tcatagtata aatataaggg tgtcaaattt
     7981 aattaaacta acatagtgta gagtatctac aactattagt ttgattatac ttgacaccct
     8041 tattttaaat aaaattttat tattataaac atgtttataa tgttctaact gtaattaaat
     8101 ttgataaatt tctataaatt gatataatca atatgtaaat tgaatttagt aaattaatca
     8161 taaaaataaa atatcgagta tatagtgtta tactaactag aaatgaggtg cttatgagta
     8221 cattttatat tcaatttctt tattttgtaa tttatagttt tataggatgg tgttgtgaaa
     8281 ctacatattg ttcagtttta caaaaacaat ttgtaaacag aggtttttta aatggtccat
     8341 tctgtcctat atatggattt ggagcattag caatagttgc tactttgact ccttttgtac
     8401 acaatattcc tttgttattt ttgtttagta taattataac tagcataatg gagtattgta
     8461 cgagctttat tttagaaaaa atatttaata tgacttggtg ggattattct aaacacaaat
     8521 tcaatattca tggaagagtt tgtctggaaa actcacttat gtttggtgta ttatcactga
     8581 ttgtaatgct tatagtacat cctattgtag tagattttat aaactctatc tctaaaaatg
     8641 tgctatttat atttgctata tctatagaag tttattttat tttagattta gtaattacag
     8701 tccatacaat tttacaatta aatggtaagt taaaacaaat caatcttata attaaggaat
     8761 taaaagataa gaaagaatac tacaaattta ttacacaaga aactattgaa aacaaacttg
     8821 aatctttagt tgaaaataga ctggatactt tagaagatag gcttgaaata cttaaggaaa
     8881 aagaaaatta tcgctatgct aaaaatcgta ttgatgagct aaaaaataaa cttaatactt
     8941 tgctgtctaa ccacaaatta cttcatagac gtataatcaa agcattcccc aatataagct
     9001 ctaataaaca tacagatatt ttaaataaaa ttaaagaaaa tattaaatca ataaaaaaag
     9061 ataaataatc aaataaacac ataagttcaa taataaaaat actaatacat aagattaagg
     9121 tttagaatat tttttgtaaa atactctaaa ccttaattaa tatactatta tttttaatct
     9181 taatttttaa ttttagttta tttcactttt aactcagtaa atctgagtgt ttaaacttca
     9241 atacactgat atgctatccc atcaatatct caactgattc cctaagtgtg ctaatctctc
     9301 ccacatttcc agggaaaatt atatatggca tattaggaaa tttactttct ttgcctgtca
     9361 tccatactgg tataccaggc tttatttgtc ccattactgt agcttttttt actcttagtg
     9421 ctttagtgcc aacatcacta gaagtaattc cacctttagc tataataaag ttgggtctta
     9481 cattaagcat accaattata ctggtaactg catctgatat ttctacagaa atcataagct
     9541 gtttatcttt atcattcgta tccaggtcaa aacgctctcg tctagtataa acagcaacag
     9601 ttttaccact gcttattttt ttctctacaa cttttataac tctttttacc tcatttttta
     9661 gaccattttc ctgaagaact aagtgttgat taaattcaat aaattctatt ggatatttac
     9721 aatttttaag ttcttctaac tgttgggtag tcttatttac atgtgaacca acaagtataa
     9781 ttccaccatt agtattacct tttgacacta actcatcctt agtaagcagt tccttatcac
     9841 tcactccacc aagtactttt gttatggcag cagcgcttct aaacataaat tccttaccac
     9901 tatttactgc tcgtataaat gcaattgtaa atacctttac atctacatag tcggttgcat
     9961 ttacaataat tttattgaat ccttcagcca ttttaagttt ttctgttatg gcttcaatat
    10021 ttagttctct aagttcttca gaagaaatat atatcatatc actagatttg tactttccat
    10081 ttgtcttttc ttcacaccac tcacctatgt gggatgattt gtatccaaaa gatttatcct
    10141 ttgcaaattc agtcattcct gctggtacaa gtatttctcc ttctttaaca taatgtacat
    10201 tatctatagt aaacctacca ccttctttaa aaaatggcat tattatttca ccctgaacct
    10261 cccatgacta aagtcacagg gttcctgctt cacagaattt tgc
//
LOCUS       DAMJVM010000115        10077 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.120, whole genome shotgun sequence.
ACCESSION   DAMJVM010000115 DAMJVM010000000
VERSION     DAMJVM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10077
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.120"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            145..495
                     /locus_tag="PC523_003080"
     CDS             145..495
                     /locus_tag="PC523_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDF2476577.1"
                     /translation="MSAKVTNQYGSIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            514..2157
                     /locus_tag="PC523_003081"
     CDS             514..2157
                     /locus_tag="PC523_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDF2476578.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKDLVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVETDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGDFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGNNIEIKNADLDTNI
                     STSMDFAKSTISTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKDINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALSSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            2238..4295
                     /gene="recG"
                     /locus_tag="PC523_003082"
     CDS             2238..4295
                     /gene="recG"
                     /locus_tag="PC523_003082"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDF2476579.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDSEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKDLIIQEYMPQRIIDKYRLCSIDFAVRNIHSPNSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIINALPFKLTKAQNRALGEIIQDMDSEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQVKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGVNPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETLAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMGLFKNKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            4354..4908
                     /gene="rsmD"
                     /locus_tag="PC523_003083"
     CDS             4354..4908
                     /gene="rsmD"
                     /locus_tag="PC523_003083"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDF2476580.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKACTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            4910..5407
                     /gene="coaD"
                     /locus_tag="PC523_003084"
     CDS             4910..5407
                     /gene="coaD"
                     /locus_tag="PC523_003084"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDF2476581.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            5411..5935
                     /locus_tag="PC523_003085"
     CDS             5411..5935
                     /locus_tag="PC523_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476582.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLKEIL
                     EEVKRDKAELRPKK"
     gene            6364..7656
                     /locus_tag="PC523_003086"
     CDS             6364..7656
                     /locus_tag="PC523_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2476583.1"
                     /translation="MEGVKVGMLKKLCSLAMVAIMTTTLITGCTSGNGKDSKKDGEKE
                     KLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISAGEGPDV
                     GYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAANPGVLY
                     YNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFGNLNWNW
                     YPYLWQAGGDIFNNDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEMIESVFG
                     PGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSSAKNKEL
                     AYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKDVYRPLVVGPHG
                     VEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            7754..8671
                     /locus_tag="PC523_003087"
     CDS             7754..8671
                     /locus_tag="PC523_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDF2476584.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVTFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTIVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGAIWSLQVFDLVYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVKRDNSDF"
     gene            8687..9496
                     /locus_tag="PC523_003088"
     CDS             8687..9496
                     /locus_tag="PC523_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDF2476585.1"
                     /translation="MKTTRRILSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFLNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            9553..9726
                     /locus_tag="PC523_003089"
     CDS             9553..9726
                     /locus_tag="PC523_003089"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476586.1"
                     /translation="MEISYVLKDCTFKHNKYSVIETETWEDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTID"
BASE COUNT         4023 a         1108 c         1883 g         3063 t
ORIGIN      
        1 attttatcca aaaacttttc taggtttaaa ttataccata tttatagtat gatatgatta
       61 taattttata taataaataa gtaagaatag taaaaaataa attaagaaat gtttttaata
      121 aaaataagct taagggggtc aaccatgagt gcaaaggtaa cgaatcaata tggaagtata
      181 gaaatagata atcaagtaat agctcaagtc gtatatagag ctgcaatgga aagctatggc
      241 ttagtaggtt tagcatataa gtctaaagga atagtagagc tattaaaagg tgagaacgca
      301 actaaaggtg taagagttga ggaagtagaa gaagatgcca tagcaataga actttatgtt
      361 ataatacaat atggtacaaa tatatctact gttgccaata atataataga tagagtaaaa
      421 tatgtagttg aaaaaatgac tggcgtaaga gttactagaa ttgatattaa tgtacaagga
      481 ataagagtta agtaatttca taggaggaaa caaatgattc agtatataga tggtaaaagg
      541 ttaagggaga tgtttatttc aggtgcaaac aatctccaaa acaataaaga tttagttgac
      601 aagttaaatg tattccctgt accagatgga gatacaggaa ctaatatgtc attaacaata
      661 tcttatgcct taaaagaatt agcaaaagtt gaaactgata atatatcaga catcggaaaa
      721 gcattatcta aaggctcttt aatgggagct agaggaaact caggagttat actttcacaa
      781 ataatcagag gtatagctaa atctatagaa ggaaaaagta aattatcaac agaagattta
      841 gcaaaagctt ttaaaaatgg ttcagacaca gcatataaag ctgtgataaa accaattgaa
      901 ggaactatac ttactgtggt tagagaaagt ggagattttg caataaaaac tgcaaaaaaa
      961 gaaaaagatg tagtaaagtt tttaagtatg cttgtgaagg agtccaattc ttcattagag
     1021 agaactccag accttctaaa gaatctaaaa gaggctggtg tagttgactc tggtggaaag
     1081 ggtcttgtac ttatatatga aggaatgtta gcttctataa aaggcaataa tatagaaata
     1141 aaaaacgctg acctagatac taatatttca acaagtatgg attttgcaaa gagtactatt
     1201 agtacagatg atataaaata ttgttattgt acagaattta ttctagaaag ttctaaggta
     1261 gaagatacta aaataagaga cataatgatg gcttatggtg atagcctagc agtagttgga
     1321 gatgatggag tcataaaggt acatgtacac actaatgacc caggaaatgt acttcaagaa
     1381 gctctaaagt atggtcaatt attaactata aaaatagaaa atatgaagtt acaacatgaa
     1441 aatacattac tagatgtaga agaaaagaaa gaaaatgata gtgaaccttt agaagaagaa
     1501 aaagagtttg gatttatagc tacttctatg ggtgaaggtt tggcaaatat atttaaagat
     1561 tttggagttg accatataat agaaggtggt caaactatga atccaagtac tgaagatttt
     1621 atgaatgcta taaaggatat aaatgctaaa aatatattta ttttcccaaa taacagtaat
     1681 ataataatgg ctgctaatca agcaaaagaa ttgagtgata aaaatataat agttatacct
     1741 acgaaaaaca cacctcaagg gtttgctgca ctagtaacat ttaatggaga attaagtgaa
     1801 gatgaaaata aagaagctat gatgaatgcc ttaagctcag ttaaatcagg tcaagtaact
     1861 tttgctgtta gggatacagt gatgaacgaa attgatgtta aagaaggtaa tataattgga
     1921 attgcagaag gaaatttatt atcagcagga gattatgtgg atgaagtaac atcaaacctt
     1981 attgaaaagt tggttgatga agatactgct ataataacat tattctttgg tgaagatgtt
     2041 acagaatctc aagcaaatga actacgtact tctttagaag aaaaatttga agatgtagat
     2101 gtagagttat attatggagg acaacctctt tattattact taatttcagt tgaataatga
     2161 ttataagaac ctgtatgacc aagtagcttt tataaaattg gttgtacagg atttttgtgt
     2221 agtaggtgat atatttgttg gatttatata aagatgttca atatgttaag ggaataggac
     2281 ctaaaaaagc agataagtta aataagctgg gtatatttac actaaaagac ctattgtatt
     2341 attttccaag acaatttgaa gatagaaata atctaaagaa aatagcacaa ttagaagata
     2401 gtgagaaagt tacaataaaa gcagtaatat caagtataaa tacatttagc ccaaaagaag
     2461 ggatgacttt aacaaaaatt gatgtaaaag atgagacagg ttcggctaaa ttagtatttt
     2521 ttaataagag ttatataaaa aatacttttc gtccaggaga ttcaatatta gtttttggga
     2581 aggttaagaa aaagtttaat aacttagaac ttacatcatg tgaattagaa tatcttacta
     2641 attcacctaa gaatacatgt agatttatgc ctgtgtacca gcttacatat ggtgttacaa
     2701 ataaagaaat tatgagtata attagaacag tacttgaaga taaagactta attatacaag
     2761 aatatatgcc acagagaatt atagataaat atagattatg tagtatagat tttgcagtta
     2821 gaaacataca ttcaccaaat agtaaggaat cattaaaaat agctttatat agaattgtat
     2881 ttgaagaatt acttatacta caactagggt tgttcgtatt taaaagtgga agaaacaaag
     2941 aagatggaat aaaatttgaa acaagtaaag atttaaaaaa aataataaat gctttaccat
     3001 ttaaactgac taaagctcaa aacagagcat taggtgaaat tatacaagat atggactcag
     3061 agaaaataat gaatagattg gttcaaggtg atgtaggaag tggtaagaca gtagtagctc
     3121 tcttagcact tgcaaattgc gttttaaatg gttaccaagg tgctttgatg gctcctacag
     3181 agatattagc agggcaacat tatatttcac ttactgagtc tctaaaagat tttggaataa
     3241 atgtaggtct attaataggt agtcttacaa aaaaacaaaa ggatacagta ttggaacaag
     3301 taaagaataa tgagattgat atattaatag gtacacatgc actaattgaa gacaaggttg
     3361 aatttaataa tattggttta gttataacag atgaacagca tagatttggt gttatgcaaa
     3421 ggagcaaact ttcattgaaa ggtgtaaatc ctgatatact agtaatgaca gcaacaccaa
     3481 taccaagaac tttagcactt atactttatg gagacttaga tatttctatt atagatgaac
     3541 ttcctccagg aagacagcca atagaaacac ttgctattga aaaaagtaag agagatagag
     3601 catataacaa ccttgtaaga agagaggttg aatcagggag acaggtgtat attgtatgtc
     3661 ctctggtaga agaaagtgaa gccatagaag ccaaatcagc agttgaactt gttgaggagt
     3721 taagagcgga atattttcac gacttaaggc ttgggttgct tcatggcaaa atgaagtcaa
     3781 gtgaaaaaga tgaagtaatg ggacttttta aaaataaaga aatagatatt ttagtttcaa
     3841 caacagtaat agaagttggt gtcaatgtgc caaatgcaac attaatgata atagaaaatg
     3901 cagaaagatt tggacttgca caattacacc agcttagagg gagagttgga agaggaagtc
     3961 ataaatcgta ctgtgtacta atttatgatt ctaaaacaga tgtatgtaga caaagaatgg
     4021 ctataatgga agagaccaat gatggtttta agatttcaga gaaggattta gaaataagag
     4081 ggcctgggga attttttggg acaaggcaac atggtcttcc agaacttaag gtagcaaact
     4141 tatttaaaca tataaaaata ctgaagttag cacagcaaga agctcgatat atattgggtg
     4201 aagataataa tctacaattg aaagaaaaca tggcactcaa aaaagaaatt atagataaat
     4261 ttaaagatac actgaaagaa atttcattaa attaatttta aattatgtta aaatattact
     4321 aatgtaataa tgtgctgaag ggagattgtc atattgagag taatttcagg aaaagcgaga
     4381 ggattaaaac taaatactcc aaaaaatgaa gatgtaaggc caactacaga tagagtaaaa
     4441 gagtctctat ttaatatgat aaattcatat ataatggaaa gtgaagttct agatttattt
     4501 gctgggactg gctctttagg aatagagtgc ttatctagag gtgccaaggc atgtactttt
     4561 gtggatatca gtaaggaaag catagatatt gttaagtcca atataaaaaa ggcaagagtt
     4621 gaaagcgaaa gtattatttt aaaccttgat tttaaaactg caatagataa acttaaatta
     4681 caaaatagta aatttgacat aatttttatg gaccctccgt attataaaaa tatgtttata
     4741 gaagctattg aaaaaattga taattccaac ctattgaacg aagatggaat aatagttgta
     4801 gaacatgata cgaatgattt atttccagat aagatatgta aattagagaa aactagagat
     4861 aagaaatatg gaaatactac attaactttt tataagatgg aggcataata tggaaaataa
     4921 acctagaaaa gctatatttg caggaagttt tgacccaatt actaatggac atttagatat
     4981 tatttgcagg gcttcaaagt tatttgacga attacaaata ggagtattaa ataatcctaa
     5041 taaaaagggt ctatttagct ttgatgaaag agtaaaactt atagaaaaaa gtacaagcca
     5101 tttaaataat ataaaagttg tgacttttga tggtttatta ataaattact gtcaagaaaa
     5161 tggaattgga gcactagtta gaggcgtgag aagtggtgct gatgttgatt atgaacttca
     5221 gatggcgcat atgaatagag agcttaatcc agatatagag actataattt taccaagttg
     5281 tacaaaatat tcatttataa gttcatcttt aataaaagag gttttgctct ttgatgctga
     5341 tataaaaaat ttggttccaa agattgtttt ggaagagtta aagaaaaaaa ctagtggggg
     5401 taattgatat atgaagatag atttagaaat gattgaacta tttgagcagt tagaggagat
     5461 atttagaagt gcttctacaa ttcctttctc acataaatgc acagtggata aagacgaagt
     5521 tttagcaata attaatgata taagaacttt aataccagaa gaagtaacac aggcagtatg
     5581 gattaataaa gaaagaaata aaataataag tcaagctaag caagatgcta caaatatagt
     5641 tgaacaagct aaaaaagaag ctgagagaat acaacaagaa tatcaagaca acatagagga
     5701 attaaagaaa aattctgaag atgtagtaaa ggcatatgta gaatccagtg aacctgttgt
     5761 tcaagcagac caaagagcta aagatatagt tgatagagct gagagaatag ccaatgaaat
     5821 aagaattggg tcaattgaat atgcagaaga tgtgctttct agtgttgagt ataatttaaa
     5881 agaaatatta gaagaagtaa aaagagataa agctgaactt agacctaaaa aataaatttt
     5941 aaataaataa aaatggagct atcacacaaa aaattctaat tgatgtgtga tagctctctt
     6001 ttaatacaac aattttttgt tatctaattg tacttttcat atcacttata tttcattttg
     6061 atattataat gcaatttaat cttttatttg tttaaatcag atgctgtatt aataggattc
     6121 ttaattgcta tgttaacatc tacagctttt tattattgta tattaaaaag atgagtaatt
     6181 agtttttaaa tgggtgaaaa tagattggca aatatcaatt gtaaatatta gaaacgttgt
     6241 catagaaaaa aactatatta aaaaaaagta catagaaaaa actgattaga caataaatac
     6301 atgctttgat aatattttaa atggaataaa aatgtgtaga attataatga aaaagaaaat
     6361 aaaatggagg gagtaaaagt gggtatgtta aaaaagttat gttcactagc tatggtggca
     6421 attatgacta caacacttat aacagggtgt acaagtggaa atggaaaaga ttcaaaaaag
     6481 gatggcgaaa aagaaaaact agttctttgg atgccaccag tagaaaataa tatgaaggaa
     6541 gtttgggacc ctattttaga taaatttgag gaaaaaaata actgtgaagt agatttacag
     6601 ataattcctt gggataatta ttctgaaaaa tttgcaacag ctataagtgc tggcgaaggt
     6661 ccagatgtag ggtatatgta tgcagaaatg tatccacagt ttatagaaag tggagcagta
     6721 gaagatttga ctaattatgc aactaaagaa gataaagagc aatctattta tataaaaaca
     6781 tctgaaatga tgggtggaat gtatggtatg ccattccaag cagcaaatcc aggggtacta
     6841 tactataaca aggatatttt agagaaatta ggagaaaaac ctcctaaaac atgggaagat
     6901 tttaaaagaa tttgtaaaaa agcaacacaa gatactgatg gtgatggaaa gatagaccaa
     6961 tggggattag ctcagggatg gggagctaaa acttttggta acttaaattg gaactggtat
     7021 ccttatttat ggcaagctgg tggagatata tttaataatg atttaaaatc agttaaattt
     7081 aatgataaga gtggtttaga agcagctaat ttcttaaaag aattacaagc atatgtgcct
     7141 gaagattctt tatctaaaga tagtaatgaa atgatagaaa gtgtatttgg cccaggaaaa
     7201 gcagcattta caataatgtt atcttctgct gcaacaagtg tatttgatga atcattccca
     7261 gacttaaatt ggggatttgt aactggacta gaagataaga aagcagcaac ttttggagca
     7321 gtagaccatt tatctttgat gtcatctgcg aaaaataaag aacttgcgta caagttaata
     7381 caacatatgt taagtgttga atcaatgaca gaatttcata aagcaatacc aagagcacca
     7441 ataacaaaag gtgaaccata tcaaggtgat gaaagattca aagaaatggt ggaaaatgac
     7501 aaggatgtat acagaccatt agtagtagga cctcatggag ttgaaatata tgaatattta
     7561 tggaaagaac ttcaaactat gatttcagga gataaaactc caaaacaagc attagatgat
     7621 gctgcaaaat attcaaatga tttattagca caataaaagt ttaaagctgt aatatgttgt
     7681 aaagtacttt acagattaaa attagcattg tatttataaa tacaatgcta atatttatat
     7741 tggagaggat aaaatggaaa acttagttag agagtttgaa gaaaattcaa aaagaaaaaa
     7801 aacaaaatta aatattaaac catatatgta tatattgcca ctgggaataa ttttagtgac
     7861 gttttatgtg ataccaataa taatgtcaat atactttagt tttacaaagt ataatattat
     7921 tagtccagct acttttatag gtttagaaaa ttataaaaaa ttatttacag atgaaacatt
     7981 aaaagtatct attataaata ctattaaatt tactattgtt gtagtacctt gccaaactat
     8041 attatcattg attttggcag tttggattac aggaaaggga aatagtaaaa tagcttcatt
     8101 tgctaaaggt gctatattta taccagtatt atcatctatg gttttgatag gtatggtatg
     8161 gagagcttta ttgaatggag aaggttctat tatatatcag gtattaggtg cttttggaat
     8221 agagtcatct aaacttttag gagatagtaa gacagcatta cctacactta tgtttatatc
     8281 tatgtggaaa aatatagggt atttcatggt gatttatatt tcagctataa tgaatttacc
     8341 aaaacactgc tatgaagtag ctaaggttga tggggctaca aaacttcaag aatttataaa
     8401 aataactgta cctttattga aaccaactac aataatggtt gtatttttag gggcgatatg
     8461 gtcactacaa gtatttgatt tagtttatac tgtgacaggt ggaggcccag gtatatctac
     8521 tatgagtata gttatgcatg catttaactt aaactttaaa aactttaatt ctggatatgc
     8581 aatgactgta gcaaacgtat tatttttatt aatagcagta gtttctattt tacaaaataa
     8641 acttgtaaaa cgagacaatt cagattttta gaaaggtagg tataatatga aaacaacaag
     8701 aagaatattg agtgatggtt tacttttatt tatagcgtgt gcaagtttgg taccttttat
     8761 atacatgttg ataatatcat tgaagataac atataactca tatagtttgg atatatcttt
     8821 ctcaacagta acccttcaaa actatataga tatctttact aaaaaagggt ttgcacaata
     8881 ttttttaaat acagcaatag tatctttttc aggtgtatta ttaaatttag tatttagtac
     8941 attagcagga tattcttttg cgaagatgga ttttaaaggc agtgataagt tatttttatt
     9001 tatgataatg actttaatta taccttctca agttacaatg ataccactat atataataat
     9061 gaaacattta ggatggataa atagttatct tgcacttatt atgccaatac caactgcatt
     9121 tggtgtattt ataatgagac aagctatttt aggagtgcca aaagagttat tagaatcagc
     9181 aaagatagat ggttgctcgg actttagaat tttaatacag atagttttac cactaatcaa
     9241 accagcatta attacactag caatatttac ttttatggga gcgtggaatg agtttatgtg
     9301 gcctcttata gcgacaacta aggatgcaat gagaacatta acagttggat tatctactct
     9361 taagacattc cagataacta attatggaca gatgatggca ggtgctacaa taacattttt
     9421 acctccattt attttctatt tgattttgca gagtaaattt gttgaaggtg tgtctttatc
     9481 tggcataaaa ggttaaatag tataatttaa taatataaaa taaacttata taaatatgga
     9541 ggaatttaaa ctatggaaat ttcatatgta ctgaaagatt gcacatttaa acataataaa
     9601 tactcagtta tagaaactga aacttgggaa gataaggatt tggatattgt agtagctaaa
     9661 ggtgaaaaat ttgcatttca aattatgcta aaagcaacag aagaatttaa ttgtacaatt
     9721 gattaaaaaa tagctacgca cactcgtgac tttagtcatg agttagtagc taaattagtc
     9781 agcatatagg gaaacctata tgtagagata ggatagaacc tatccaatac tacttgaatt
     9841 gctggaaacc cctaaagcta accaaactaa aacataagga tgaaataaac ctaagtgtga
     9901 aagttgcgaa agtagaaaaa attggttaga tggtataagg ttaaacccta agtactttaa
     9961 aatgggcaat cagcaggtaa actccgaata ggagaaactt caacgactat tcctcttgag
    10021 ggaagtacac tacaagctat tggtagtgga agtgggtaga ccttaacgga taatgcc
//
LOCUS       DAMJVM010000116         9717 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.121, whole genome shotgun sequence.
ACCESSION   DAMJVM010000116 DAMJVM010000000
VERSION     DAMJVM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9717
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.121"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            256..1317
                     /locus_tag="PC523_003090"
     CDS             256..1317
                     /locus_tag="PC523_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HDF2476587.1"
                     /translation="MRDFLKNYKSSIILLACTIIGAIVGLVLGPKASILKPFGDLFIN
                     ALLMIVVPLVFLSVSSGVASIKNMERFGKIMKSTAIVFMGTTLVIAFLGITSALLFNP
                     SKGLDVSSVKSLMVAQDAETLESKGTLLDQVVSSITVKNFGDLFSSSNLLQLVIFAVL
                     VGTAIAMMGDRGNTIRKLLSEGTEAILKVVSIVMLYAPIGLGCYTATIIGELGSKIIQ
                     GYVRVFVIYSLTAVMYFLLFYSLYAFLSAGKQGFRQFWRNAIKPIATALATSSSSACI
                     PSNLEASKNIGVPDDISETVIPLGINIHKDGSVLAVIIKVAFLSGILGSGMANPATIG
                     QLFITVFVVGVFMAPVAGG"
     gene            1330..1527
                     /locus_tag="PC523_003091"
     CDS             1330..1527
                     /locus_tag="PC523_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDF2476588.1"
                     /translation="MLVVSIFGFPVEAIPLLTVITTITDIPNTILNTTGNTVSSMLVS
                     RLVEGKDWLIDKTAITTKKIS"
     gene            complement(1677..2381)
                     /locus_tag="PC523_003092"
     CDS             complement(1677..2381)
                     /locus_tag="PC523_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2476589.1"
                     /translation="MQQGSDYLKKILEKHIDLLKKIPLFEGIKSDELEAMLECLGVVD
                     KIYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEILFIKFKSVTGICSSTCVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSNKFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFYKNKFEIFCFQK"
     gene            2542..3267
                     /locus_tag="PC523_003093"
     CDS             2542..3267
                     /locus_tag="PC523_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2476590.1"
                     /translation="MMRKIIKIDEKKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKTQNKNLACGCPGSQSKSINRE
                     GSNNASISNDVVETKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEIDYTEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            3326..4918
                     /gene="hcp"
                     /locus_tag="PC523_003094"
                     /gene_synonym="priS"
     CDS             3326..4918
                     /gene="hcp"
                     /locus_tag="PC523_003094"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDF2476591.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGDTANLQDELTGVLIGL
                     SRATIGNKNRPTSKTDKIMIEGLFTTITNVSFDDEAIKRQIEKTEIEKSKIVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYKEDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGDIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            5296..6201
                     /locus_tag="PC523_003095"
     CDS             5296..6201
                     /locus_tag="PC523_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2476592.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKHIFNKSYTEMLRPKYENKNLKEVLLRIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            6325..7278
                     /gene="msrA"
                     /locus_tag="PC523_003096"
     CDS             6325..7278
                     /gene="msrA"
                     /locus_tag="PC523_003096"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDF2476593.1"
                     /translation="MVKLATFAGGCFWCMVKPFDKYDGVIKVISGYTGGHIENPTYQE
                     VCSGTTGHVEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESKEHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPKEDMETLGYGEYLKLFE"
     gene            7431..8561
                     /locus_tag="PC523_003097"
     CDS             7431..8561
                     /locus_tag="PC523_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2476594.1"
                     /translation="MNLKDFNGKKLKSARTFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINVVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPSRKVMSDININDIESLAYKTREFWDLNSTPIVNMVNLLES
                     KGIIISGMNVDRKWATIFTQKQRISKESKYIISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPNKQFSEDEYACAFLLPKDIFLEDLIHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LGKINYKKYNYLVNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLDEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDSKK"
     gene            complement(8678..9646)
                     /locus_tag="PC523_003098"
     CDS             complement(8678..9646)
                     /locus_tag="PC523_003098"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDF2476595.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFIVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRLLSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPNTYSNLDLP
                     SIVEKNKKTGINIINGKDCTEFGIGTALVEIVKAILHNEKKILPVSTLLENQYNERNV
                     FASVPCVIGKDGIEEIIEINMTEDEQNEFNKSCSVLREYIALSKTL"
BASE COUNT         3730 a         1126 c         1639 g         3222 t
ORIGIN      
        1 gtttgtttta ttaaaatcaa tctagaaaaa aagttttaaa agcagaattt tattaatatt
       61 tatatatagg tatgaactat taaaaaaaga ttaaagatac aaaaaaacta tagaaattga
      121 catatattcc agtatttgct aaagtagctg ttggggaaaa ttacaaaaac tattaatgcg
      181 cattagataa tttactttaa tttttttagg gtttggggta atttataata taaaatatta
      241 gaaaggaaaa tataaatgag ggacttttta aaaaattata agtcatcaat tatactttta
      301 gcatgtacta taataggggc gatagtagga ttagtacttg gtccaaaagc ttcaatacta
      361 aaaccatttg gagatttatt cataaatgct ctcttaatga tagtagtacc attagttttt
      421 cttagtgttt cttcaggtgt tgctagtata aaaaatatgg aacgctttgg aaaaataatg
      481 aagagtacag ctatagtatt tatgggaaca acacttgtaa ttgcattttt aggtattaca
      541 tctgcattat tatttaatcc atctaaagga ttagatgtat caagtgtaaa aagtttaatg
      601 gtggctcaag atgcagaaac tttagaatca aaaggaacat tattagacca agtagtatca
      661 agtattacag ttaaaaattt tggagattta ttttccagta gcaatttact acaattagtt
      721 atatttgcag ttttagttgg aacagcaata gctatgatgg gagatagggg aaatacaata
      781 agaaagcttt tatcagaagg aacggaagca atattaaaag tagtaagtat agtcatgctt
      841 tatgctccta tagggctagg atgttataca gctacaataa ttggggaact tggaagcaaa
      901 ataattcaag gatatgtaag ggtgtttgta atttattcgc taacagcagt catgtatttc
      961 ttattatttt acagtctata tgctttttta tctgcaggaa aacaagggtt tagacaattc
     1021 tggagaaatg ctataaaacc aatagcaacg gcactagcta cttcttcaag ttcagcatgt
     1081 ataccatcaa atttagaagc tagtaaaaat ataggagttc ctgatgatat atcagaaaca
     1141 gttatacctc ttggaataaa tatacataaa gatgggtcag ttttagcagt aataataaaa
     1201 gtggcatttt tatcaggtat tttaggtagt ggaatggcaa atccagcaac gataggtcaa
     1261 ttatttataa ctgtatttgt tgttggagta tttatggctc ctgtagcagg tggctgactt
     1321 attggtgaga tgctcgtggt tagcatcttc ggttttccag tggaggcaat tccattgtta
     1381 acagttataa ctactataac tgatattcct aatactatat taaatacaac tggaaataca
     1441 gtatcatcaa tgttagtttc aagattagta gaaggaaaag attggcttat agacaaaaca
     1501 gcaattacta caaagaaaat aagctaatta tatacttaat taataaagtt aatagatatc
     1561 ttaattttaa taaattagat atctattaac tttttaaata aaaatataaa tttaagattc
     1621 aaatatgata tttataattt gaattaacta atataatata tgtttaaata tctagtttat
     1681 ttttgaaagc aaaagatttc aaacttattt ttataaaaag ataacaagcc ttcatcccgc
     1741 atcttaccaa gttctcttga catagaactt ctgtcaacac acaaaaaatc agccatttca
     1801 tttcttgaaa aagggattgt aaacttgttg ctacctgttt gttctatctg ctttgaaaaa
     1861 tacataagca atttttcacg agttgtttta cgagaaagca attctatttt attgtggagt
     1921 aaaatatttt tttcggctat taattgtagc atgttttcaa caagtctatt atgaaataca
     1981 catgtagatg aacaaatacc agttacactt ttaaacttta taaataaaat ttcacaagaa
     2041 gatgttgtgg tcactgtgac attactttta tgtagtctag tacatgcaaa cacctctcca
     2101 aataaatctc caggtaagag ttcagctacg atatttctat taccttcaat atcttccttt
     2161 attatatgtg cacttccttt aagcataatt cctattgaag taacctcact tccaacagag
     2221 aatattatgc tatcttttgg ataaatcttg tctacaacac ctaaacattc taacattgct
     2281 tcaagttcat cacttttaat tccttcaaat agaggaattt tttttagtag gtctatgtgt
     2341 ttttctaaaa tttttttcaa ataatcactt ccttgttgca tatgcaaccg ataatttgtt
     2401 tttattgtac tatactaagt ttataaaagc aaggagataa aaatactact ataagaaatt
     2461 tgaaataaaa agatgtagtc ttaaaaataa atatataaga agtaaatatt taaaattaaa
     2521 aaaatttatg aggtgatata aatgatgaga aaaataatta aaattgatga gaaaaaatgt
     2581 aatggttgtg gactatgtgt tgaagcttgt catgaagaag ctataggaat ggtaaatgga
     2641 aaagcaaaac ttttaagaga tgactattgt gatgggctgg gagattgcct accaacttgt
     2701 ccaacgaatg caatttcatt tgagtataga gaagcagcag aatatgatga agcagcagta
     2761 aaagcgaata tggaagcaaa aaaaacacaa aataaaaatt tagcatgtgg atgtccaggt
     2821 tctcagtcta agtccataaa tagagagggt tcaaataatg catctataag caatgacgta
     2881 gtagagacaa aaggttctca acttaaccaa tggccagttc aaataaaatt agtaccaact
     2941 aatgcatctt atttaaagaa tgcaagcctt ttaatagcag cagattgtac agcatatgca
     3001 tatggaaact ttcataataa atttatgaaa aataaggtta cattaatcgg atgccctaaa
     3061 cttgatgaaa ttgactatac tgaaaaactt acagagattt taaaagaaaa tgatattaaa
     3121 aatatagtag taacacgtat ggaagttcct tgctgtggtg gtatagtaaa tgctgttaag
     3181 acagctctac aaaatagtgg aaaaatgata ccatggcaaa tagttactat atctgtagat
     3241 ggaaaaattg tggatggaga aatttaatta agtgaattac agaaaaattt aataaatttt
     3301 aataaacaaa agggaggaat aaaaaatgga aaaaatgttt tgttttcaat gtgaacaaac
     3361 agcaggaggt aaaggttgta ctataaatgg tgtttgtggt aaaaaggggg atacagctaa
     3421 cttacaagac gaacttacag gagtgttgat tggtctttca agagcaacta ttggaaataa
     3481 gaatagacca acaagtaaaa cagataaaat tatgatagag gggttattca ctactataac
     3541 aaatgtaagt tttgatgatg aggctataaa aagacaaatt gaaaaaacag agatagaaaa
     3601 aagtaaaatt gtacctagat gttcagattg ctcaacaact tgtaatagaa atgataacta
     3661 tgatatgaaa gatttatgga atgataatga agatatacgt tcattaaaat cattaatatt
     3721 atttggatta agaggtatgg ctgcctatgc atatcatgcc atggtactag gttatacgga
     3781 caaagacgta aatgagtttt tctatgaagg attaatagca gttggaggag acttgtctat
     3841 tgacgaactt ttaggtttag ttatgaaaac tggagaaata aacttaaaat gtatggaatt
     3901 gcttgataga gcaaatactg aaacttatgg aacgccagaa ccgactcagg tatctatgaa
     3961 aatcgagaaa ggaccattta ttgtagttac aggacatgac ctttatgatt tacacaaatt
     4021 attagaacaa acaaaagata agggtataaa tatatatact catggtgagt tattaccagc
     4081 acatgcatac ccaaaattaa aagaatataa acatctaaaa ggtaattttg gtacagcatg
     4141 gcaaaatcaa caaaaagaat ttgataatat tcctgctcca atactattta caacaaattg
     4201 tttaatgcct gttaaagata gttatagaga tagagtattt actacagaag tagttgctta
     4261 tccaggtatg gttcatattg atgaagacaa agatttttct agtgtaatag aaaaagccct
     4321 tgaacttgga ggatataaag aagatgttca tatgactggt attaatggag gagatacatt
     4381 aactactggt tttgcaagag gtacagtttc atctgtacaa gataaagtag tagatgctgt
     4441 aaagagtgga gatataagac actttttatt ggttggaggt tgtgatggtg caaaacctgg
     4501 aagaaattat tacactgaat ttgtaaaaca agctcctaaa gatactgtta tattgacact
     4561 tgcatgtgga aaatatagat ttaatgattt agaccttgga gatataggtg gcttacctcg
     4621 tttaatggat atggggcaat gtaacgatgc ttatagtgca attaaagtgg cagtttcatt
     4681 ggcaaatgct tttgaatgtg gagtaaatga tttacctctt agtatggtac tatcatggta
     4741 tgaacagaaa gctgtttgta tacttcttac attacttcac ttaggtatca aaaatatcta
     4801 tataggacct tctttaccag cattcttatc taagaatgta ttagatatat tggttgataa
     4861 atttgcattg acgcctatat ctactcctga agaagatatg aagaagattt taggataaaa
     4921 aaataaatat atctatactt aaatctattt atagttaaaa tatttttaag tatagatgca
     4981 attaagataa ggggttaaac ttaaaatttg attaaaaaaa tcaattttga gacaaccctt
     5041 tatttatttt ttgtaataat tgaaaattaa acctgctttt agtatattct ttttaggaat
     5101 ccttgtatta aacttataat gcttagatat ataatatctt atagatttta ttttaagtag
     5161 taaaattttt aatggaattt aaatattaaa aaaggaacat tgtaatatgt aacttgcata
     5221 aaatatatat actatagtga aattatttta attaaaattt aaaatagaaa gtgttttatg
     5281 tgggggtata tgtatatgaa aaattctaat ttcaatatat tggcatttga tggtggtgga
     5341 ttaaaaggag cattgagtat atccatactt gagaggatag taaaagagta tccaaatcta
     5401 ttaaataata taaatatgtt tggaggaaca tctacaggaa gtctaattgc attaggactt
     5461 gcatatggtg taagcccgaa agagataaaa gagctttact caatcgaaaa ttcaaaacat
     5521 atttttaata aaagttatac agaaatgctc agaccaaagt atgaaaataa aaatttaaaa
     5581 gaagttttat taaggatatt tccagaggaa ctagaattaa aagatttaaa taaattagta
     5641 atgataccaa gtttttatat aggaaatgaa gaaaatgcat ggaaaccagt gttttataac
     5701 aatatgccaa attcttttac aaagactagt aaagtagtag atgtagcaat ggcaagcagt
     5761 gctgcaccag tgttttttcc aacatataat cgccatgttg atggaggaat aatagcaact
     5821 gacccaagtc tagcgtgtat aattcatgcc attgacagtg gatttaagct taaaaataca
     5881 agactttttt cgataggaac tggatatgta tataatagta taaaggcaga cactactgaa
     5941 tggggagcta ttgactggat aattaataaa gaacctgatt taccaattat atctattaca
     6001 ttagaaggaa attctcaaat gagtcagtta ttttctcaaa aacttcttgg agataattac
     6061 tatagattaa atcctaagat ggaaaaagat gtagctatgg atgattgtga tgcgttagaa
     6121 tatttaatgt ctttaggaga aaattgtgat ataaaagatg cttttgggtg gataaagaaa
     6181 aagagctttt atagaaatta gattcaattt aaaattgtat ttttacacat gatttcattg
     6241 aatatgtaaa aataataaaa tgcagattat taagttactt cttagggaat aaataaaata
     6301 taatttattt aaggagtgat gaaaatggta aaacttgcta cttttgcagg tggatgtttt
     6361 tggtgtatgg taaaaccttt tgataagtat gatggagtta ttaaagtaat ttctggatat
     6421 acaggtggtc atattgaaaa tcctacatat caagaggtgt gttcgggaac aactggtcat
     6481 gttgaagcta ttcaaataac ttatgatgat gatataataa gttatgatga gctactgaat
     6541 atattttgga aacagattaa tcctactgat gatggtggac aatttggtga taggggaact
     6601 caatataaga ctgctatatt ttatcatgat gaagagcaaa aaaatatagc tttagagtca
     6661 aaagaacatc tagaaaatag taaaatattc gatagtaaaa tagtaactga tataattcca
     6721 gcaactaagt tctatgaagc agaagaaaat catcaggatt attataagaa aagaccatta
     6781 aactacaata tgtattataa agggtctggt agatataatt ttgtaaaaag tcattgggat
     6841 ggaaacaata taaatagaga agagttaaaa aagactttaa ctcctataca atttgaagtc
     6901 actcaaaatg atatgacaga ggtacctttt gaaaatgagt attatgataa taacaaagaa
     6961 ggaatttatg ttgatattgt aagtggagaa gtgctatttt cttcaaaaga taaatttgat
     7021 tctggatgtg ggtggccaag tttttcaaag ccagttatag ataaatctat tattgagaga
     7081 agtgattttt ctcaaggtat gtacagagtt gaggtaagaa gtgctaaagg aaattctcat
     7141 ttaggtcatg tatttgatga tggtccaaaa gagcttggag gtttaagata ttgtataaat
     7201 tctgcttctc ttaaatttat acctaaagaa gatatggaaa ctttggggta tggagaatat
     7261 ttaaaacttt ttgagtaatt ggaactattt attataaact tagctaaaag gtgaaaatat
     7321 aagaaaatat tttcaccttt tgtttttttg tcaataatat gataaattat taattgtaat
     7381 cttatttata ccagtagatt aagaatttaa ttttgaaaga gagaaaacaa atgaatttaa
     7441 aagactttaa tggaaagaaa ttgaaatcag ctagaacttt tagagcaaag acgatagaac
     7501 agttgtctaa agaaactaaa ataaacaaaa aagatttaaa agcttttgag gaaaataagt
     7561 atgtcccaaa tatagaaaat acattaaaat tatctaatat attaaatttt ccaaaagagt
     7621 atttttataa aaatgaaaat ataaatgtag ttgtagagga tagccacttt aatcctcaat
     7681 ctaaactacc tagagtagaa gaaatttctt atagagaaaa aataattata atacataaaa
     7741 tatattcttt tatggagaat tatattaagt ttccaaaaga aaacttacca agtagaaaag
     7801 taatgtcaga tattaatata aatgatatag aatctcttgc atataagact agagagtttt
     7861 gggatttaaa tagtactcca atagtaaata tggtaaattt gttagagtct aaagggatta
     7921 taatatcagg tatgaatgta gatagaaaat gggcaactat ttttactcaa aaacagagaa
     7981 ttagtaagga atctaaatat ataatatctc tcggtaatga taaaaagtca gcatcaatta
     8041 gaaattatac tttggcatgt gagcttgggt atataatatc aagtgaatta agaataccaa
     8101 ataagcaatt ttcagaagat gagtatgcat gtgcattttt attgccaaaa gatatttttt
     8161 tagaagattt gattcatcca gaagatttgg attattatgt agaactaaag aaaaaatgga
     8221 ttgtacctat atctgctatg atttttagag cgtataatct aggaaaaata aattacaaaa
     8281 aatataatta tcttgtgaat gaaatggata aaaaaggctg gttaatagaa gaaccactag
     8341 acaaaatgaa aggttcaagc ccaacgtatt taaaaagagc agttgaatta ttgatagaaa
     8401 ataaaataat gagcgtaaat agtatagtat caagtttaga tgaatttgga ataaatcttt
     8461 atccagaaga cttagaatta ttgatgggat taaaaaaagg actgttatcg caagaggtaa
     8521 ataaaaaaag taaagttata aaatttgata gtaaaaaata ggttgatatt ttagcattaa
     8581 aattaaaagc aggtataaat gatttaaaca ttaatttaat taaaagtctt ttcaatgagt
     8641 catttacctg cttttagtgt aaaaaagaat cttgaatcta aagtgttttt gaaagtgcaa
     8701 tatattctct taaaacagag caagatttat taaattcgtt ttgctcatct tcagtcatat
     8761 tgatttcaat aatttcttca ataccatctt ttcctataac acatggaaca cttgcaaata
     8821 catttctttc attatattga ttttctagta atgtagaaac tggaagaatt tttttctcat
     8881 tatgtagtat agcttttaca atttctacta aagcagtacc tataccaaat tctgtacaat
     8941 ctttaccatt tattatattt atccctgttt ttttattttt ttcaactatt gatggtaggt
     9001 ctaaattgct ataagtattt gggttatcct ttatcatgtc caagattggt ttaccaccaa
     9061 ttgaaacatg agaccaagga accatttgag aatctccatg ttcacccata gaataagctt
     9121 gaattgattg ttgagcgatt ccagtttctt cagataaaag ccttctaagt cttgttgaat
     9181 ctaaagctgt gccagttcca atgactttgt ttttaggaaa gccagatttt tcccaaatat
     9241 aatttgtaac tacatcaaca ggattagata tgactataaa tataccatca aagccagaat
     9301 caactatggg ttttactata gattttatca ttccaatagt tggtcttagt aaatctaatc
     9361 tagttgtatt ttgtctacgt aaaggacctt ctgaagagtc tgcaacactt atcactacta
     9421 tatcagaatc tttacagtca gaaaaagttc ctttttctat atgtactttg tgtggcagat
     9481 atgaaactgc atcagctaaa tctaaagctt gagcttttga tttagattca tctatatcaa
     9541 tcataaagag ttcatcacaa actccttgag ttattaaact gaatccacaa tgagagccaa
     9601 catgccctga acctacaata gatatttttc ttggctttat tgccatttta aatcactccc
     9661 cctaaaataa cgatacattt ataagaatat tatatattat aaagagataa tgtataa
//
LOCUS       DAMJVM010000117         9664 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.122, whole genome shotgun sequence.
ACCESSION   DAMJVM010000117 DAMJVM010000000
VERSION     DAMJVM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.122"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            139..1029
                     /locus_tag="PC523_003099"
     CDS             139..1029
                     /locus_tag="PC523_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2476596.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            1247..2533
                     /locus_tag="PC523_003100"
     CDS             1247..2533
                     /locus_tag="PC523_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2476597.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKKDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHINDLCDKYKDRLFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTKENLLDILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKAFGKNEEIVINPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDNAKQMTKEIEETIF"
     gene            complement(2622..3065)
                     /locus_tag="PC523_003101"
     CDS             complement(2622..3065)
                     /locus_tag="PC523_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HDF2476598.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFSITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDKDKKKP"
     gene            complement(3089..3883)
                     /locus_tag="PC523_003102"
     CDS             complement(3089..3883)
                     /locus_tag="PC523_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HDF2476599.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEIAAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKRIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(4449..4925)
                     /locus_tag="PC523_003103"
     CDS             complement(4449..4925)
                     /locus_tag="PC523_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2476600.1"
                     /translation="MKEILINPIGKIRIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTTVQIISIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSIDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(5026..6195)
                     /locus_tag="PC523_003104"
     CDS             complement(5026..6195)
                     /locus_tag="PC523_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2476601.1"
                     /translation="MLNIRTLCNEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVNPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLKNPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIKGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIKE
                     ICDFTCKTFGGSISVEIEEGYPALINSNYLVDYVKQNAVELFGKENIILKDAPTLGAE
                     DFSYFLKHCEGAFYHLGCANREKNIISPLHTSTFDIDEGCLITGVMLHVKNVLAF"
     gene            6358..7221
                     /locus_tag="PC523_003105"
     CDS             6358..7221
                     /locus_tag="PC523_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDF2476602.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLSYKGYL
                     AGNDDIRARDIEGMFLDKDVDMIMCLRGGYGTTRILDKINYEIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVAKWDEFTYKSLVNALEFKDDLYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEEKDLIELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            7292..7885
                     /locus_tag="PC523_003106"
     CDS             7292..7885
                     /locus_tag="PC523_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HDF2476603.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(8137..8772)
                     /locus_tag="PC523_003107"
     CDS             complement(8137..8772)
                     /locus_tag="PC523_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2476604.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAK
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTSKSIELLSYPVHCLENDIES
                     ELVDELKSIENLKILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(8852..9583)
                     /locus_tag="PC523_003108"
     CDS             complement(8852..9583)
                     /locus_tag="PC523_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDF2476605.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLEIDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLKKLKTLNSSFEGYQNNKECKIPTLEEVLILISPTD
                     LILNIELKTDKVNYPNIEKDVLELILKYNIKNRVLISSFNSNSLKNFHKLDPSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIEICHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLQSKQYR"
BASE COUNT         3644 a         1165 c         1440 g         3415 t
ORIGIN      
        1 aagatttatt agttacttga catagtttac acttttaatt atagtataaa ttggaatatt
       61 tagataatta ttatttaatg tacaagctaa gtttttaatt aatacggctt tatacattta
      121 tatagaaaag aggtacgtat gagtagcaca atcaaatcaa atcgaataaa aggtataatg
      181 tttataatag catcagctct tggctttgca atgatgtctg catttgtaaa actagctgga
      241 gatttaccaa gttttcaaaa agtgttcttt agaaatctgg tatcagctat aatagctttt
      301 tggcttatca taaaacataa aggtagtcta actgggaaaa aagaaaattt aaaagtactt
      361 ttatataggt caatatttgg aactctagga gttatattta actactatgc aattgacaag
      421 ttgatgcttt cagacgctaa tatgctaaat aaaatgagtc ctttccttgt agttatattt
      481 tgtgcaattg ctttaaaaga aaaaataaat gtaaagcaga taggagctat tataattgca
      541 tttataggag ctttattcat aataaaacca acatttagtg tagaagtaat tccatatctt
      601 ggaggaattg caggagctgt atttgcagca atggcatata cttgtttaag agttcttgga
      661 gatagagaag attactatac aatcgtattt ttcttttcgg ttttttcatt agttactgta
      721 gggccaattg cttttgcagt atatgaacca atgactctta tgcaacttat atatctatta
      781 ctaggtggag tatttgcaag cctaggtcaa tttggaataa ctctagcata taaatatgca
      841 cctgctaagg aaatatctat attcgactac agtaatataa tcttttcagc aattttaagt
      901 atattcttat tcaatgttta tccagatatg ttaagtgttg ttggatattt aatagtattt
      961 tcagcagcat tttatatgtt tttatataat aagaaattag ataaattaga caaagaaaag
     1021 aagaagtaag ctagataaaa gtgtttttat aatttgtatt gaatatttaa gtctttgtat
     1081 taaaattaaa gaaaatttaa ttaaaaatgt ttggtatatt aacttataac agcagttaaa
     1141 attgaaaaat tataactgct gtttctttta ttaaaaatat ccaaatatcc gtaaaaatag
     1201 actataatag agtagtataa ataatataaa ggggataaat taaagtatgg aaaaagtgaa
     1261 agttgctgtg attggagctg gtaatagagg aacgtatgca tatgctccat atatttatga
     1321 aaattctgat gtatgtgaaa tagttgctgt agcagagcct aaaaaaggaa gacgagaact
     1381 atttacacaa aaatataacc tagatagtaa aaatgtattt gaaactttag aagacttttt
     1441 taaaaaggac aagatggcag atgcagttat aatagctaca aatgatgata gacattatga
     1501 tgtagcaaaa ctagctcttg aaaaaggtta tcatgtatta ttagaaaagc caatgtctaa
     1561 tagtttagat ggtttggtac atataaatga cctatgtgat aagtataaag ataggctttt
     1621 tatgatttgt catgtattga ggtattctcc attttataac aaattaaaag agattgtaga
     1681 aagtaaaaaa ttaggagaac tagttagtat acaatataat gaaaatatag gatattggca
     1741 ttttgcacat agctttacta gggggaattg gagaaatagc aatgaaacaa gtcctttaat
     1801 tttagccaag agttgtcatg atatggatat tttattatat ttagtaggaa gtagatgtaa
     1861 aaaaatatct gcatttggga gcttaaagca tcttaataac caaaatgcta gtggagagat
     1921 ggcacaaaat tgtcttcagt gtttagtaga aaaaaaatgt ccatattcag caaagagaat
     1981 ttatttagaa aaagatagaa gtataaatag agctgtccat ataaatccaa ctaaagagaa
     2041 tttattggat atacttaaga caagtccata tggaagatgt gtttatagat gcgacaataa
     2101 tgttgtagat aatatggtaa atatattaca gtttgaaaat ggagtgacgg ctacttttaa
     2161 tttatgtgca tttactaaag aaaatggaag aactataaag ttaatgttta gccatggtga
     2221 agttggtgga gatttaaata aaaatgagat aagaattaaa gcatttggaa aaaatgaaga
     2281 aatagttata aatccatcaa atcaacagaa tatggtagag gaatacgata gaaatcttat
     2341 agctgaattt attaaattag tttcaaataa agaactagaa aagggtagag tagcagcaaa
     2401 agaagctata caaagtcatg taatggcatt tgcagcagaa tactctaggg ttagtgatga
     2461 ggtagtgtat atagaagaat tttttgataa tgctaagcaa atgactaaag agatagaaga
     2521 aactatattt taattaataa aaatttatta aataaaataa agatagatta agtaatagaa
     2581 atacgaaaat atgtattcta ttaagaaatc tatctttttt attaaggttt tttcttatct
     2641 ttatcaaaaa aatccataca ttcggacaaa agataagaaa gtaaagcgaa taagatttct
     2701 gtacttaaag gaatcgttac tgaatccata aaggtatcaa ttatgttcat agctagaaaa
     2761 gttagtataa aatcaattat aaattcgcaa agtttatata ttgaatatgg taatttgttt
     2821 acatatctga atatatctaa aatagtagtg catagaaaat caattggagt ggttatacaa
     2881 aaatatatta aaaagaaaat taatacagct ccaagagact tatattcaaa tcctaagaat
     2941 gataagaagc caattcctat aaccgtgaaa atacaaaaag ttatactaaa aaaaacagca
     3001 aaaaataaaa atacagaaat aaattcgcct aaaggaatcc ttcttttatt acttatctta
     3061 atcaacccct taatttatga catgaaactt atataaataa taacattctt aaaaattcat
     3121 aatagtcctc tgtttcaaat agtactgtct gagaatctat ttttttcata tttggataaa
     3181 aagaggcctt aaaagcttta taatgttctc taaattttat ttctatctta aatttatctg
     3241 gcaattttat taagtgtcta gataaatctc cagataaagc ttttttgaca cctttttcga
     3301 ttctttttag tgcaaggttt ggatggattg aaattgaacc atttccaatt ccttttgaca
     3361 caggaacagt gactatattt ggatttagtt tttttgcact ctcacaaagc atttcatctc
     3421 cacttaaaaa tgctacagga acattataat atgcagcaat ataagcattt atagtaaatt
     3481 cagatgctat ttcatcattt attttaaaat aatcataatc aatatccata gtatgagaaa
     3541 gtggattacc attacaagta gctccagaat gatagccagt gaatatacat gcatcaaagc
     3601 tttcatcaat cccagccatc ataactagtg ggtctttggt ccatccccta agtattttga
     3661 cgttttcagg taaaatattt ggattaatat ttctaccact atcatgtgca tctttaacaa
     3721 gtatctcatg cacaccagat tgagtagctc catcacatgc tgctgctatc tctttactca
     3781 tttgtgttct atagtgttgt gaaaattcac attcaagttt tgtctcatcc caatttacaa
     3841 taccacaagt accttctata tcagcactta aatataattt cataatatat acccccttat
     3901 gtttattatt aatgataata acagataaca gaaaatacat aatcaataaa gggcataaaa
     3961 acattatgtc ttactattaa ttattattaa atctgtaact gaaaaataac taaactggta
     4021 ttaaaaaata taacactatg tgtcatttat ttatatagtt ttttatatct gatgtttata
     4081 taaaattgta acatataagt ggttacattt actagtttaa catagttaaa atagttaggt
     4141 attattttta atttaaattg attattataa aatcgtggag taaaagaaaa atttaattta
     4201 ttaaaaatgc acgaaatgtt ttatccttaa cacaatgcta aattaaactc aaaacgaaat
     4261 aatagaaaaa tcttatgttc ttagaaagaa tgtgaatgat ttaagggaaa aatagaaatt
     4321 tattatataa tattaggctg tattatataa tacagcctaa tattatatgt atatgtgaca
     4381 taaaatcttg gtaagtgtaa ataaaataaa tgtggaaaac aagttaatga aatcaagaaa
     4441 aaagtaattt aaaattcaat ttcatcttcc caattaaatt ctcctgattt ttctacactc
     4501 ttaggccagt gactacacca ttttggaact gacggatttt caaccctatc aatgcttgga
     4561 gtatatggct ttaagtcaat aacaggacta ttatcattag catcaatata tggtattttt
     4621 attataccat tttcatggtc tatactgata atttgtacag tagtcaatgc tattgggttg
     4681 ggtcttatag gtgaccttgt tgcaaatatt cctataacat ctggcccact tttataaggt
     4741 ttaggagttt ctaaaatact tcttagttca ggattatcga acccatcagc ccaccaaaat
     4801 acatttaagt ggctgaagtc gtcgagttca tttaatgcag gtacatactt tttgtctaat
     4861 ttaataaaga cctcctcgcc ttgaatacgt attttaccta taggatttat taatatttct
     4921 ttcataatat tacctccaat taattaattt atcatttgta attttagggt ataacatgac
     4981 atcatgtgag ggtcaatata aaataataaa taaaaatatt aatatttaaa atgctaatac
     5041 attttttaca tgaagcatga caccagtaat aagacaacct tcgtcaatat caaaagtaga
     5101 ggtatgcaga ggggaaataa tgtttttttc tcgatttgca caaccaaggt ggtaaaaagc
     5161 tccttcacaa tgttttagaa aatacgaaaa gtcttcagcc ccaagagtag gtgcgtcttt
     5221 aaggataata ttttcttttc caaacaattc tacagcattt tgcttgacgt aatctactag
     5281 atagtttgaa ttgataagag ctgggtaacc ttcctcaatt tcaacggaaa tactgcctcc
     5341 aaatgtttta caagtaaaat cacaaatttc tttaattcgt ttttttgaaa attctctagt
     5401 ttcaggagtc aaggttctca aagtacccct tataactact ttgtcacaaa ctatattttc
     5461 tttgattcca cctttaattt ttcctagaga aataactaca gaatcagttg gatttgtgtt
     5521 acgactaacg attgtctgta gagctgtaat aatatgagca gatataacga tagcatcaat
     5581 accttgatgt ggataagctc catgacattt tgaaccatgt acagttaatt gaagtgtatc
     5641 ggtagaagca tttagagtgt tgtattttaa ttcaatcaga tttttgttga tattgggatt
     5701 tacatgaagt ccaaatatat attttacatt tgggttttta agacatccat cttctatcat
     5761 aagttgagct ccaccaatag tttcttcagc aggttgaaaa aagaatttta cgtttacatt
     5821 taagtaatcc tttaatttaa atagtatatt acaagtacct agtaaaatag cagtgtgtgc
     5881 atcatgaccg catgcatgca tttttccaat attaatagat ttataagaat aattaagttc
     5941 ctctgttata ggtaaggcat ctatatcagc tcttattgca actgtttcaa agtttggatt
     6001 tacgttgatg tatgcagtta tccctgtatg attcttatat tcaatataat ttatacctat
     6061 ttcatttaag tacttaataa tttttttctt agtttgaaat tcttctagtc caagttctgg
     6121 agttttatgt aaatctcttc ttatatttat aacccaatca tttatctcat tacaaagagt
     6181 tcttatatta agcataaaac acatcctaac catattaaaa atttattttt ccttattaat
     6241 tatattattt tatgtaaatc attattctgt atcaagtaca aataaaatat cttataatta
     6301 tattagagat atttagtatg gagaagaata tatcatttta taagaagagg tgtttaaatg
     6361 ttaggaataa ttgcgccatc tgggccatta agaaatacaa gtttggagga aataaagttt
     6421 aacttagaaa gttatggtta cgaagttaag ttttctgaaa gttgtagttt aagttataaa
     6481 ggttatttag ctggtaatga tgatattaga gcaagagata tagaagggat gtttttagat
     6541 aaagatgtag atatgattat gtgtttaaga ggaggttatg gaacaacaag aatattggat
     6601 aaaataaact atgagataat aaaacaaaat ccaaaaccat ttataggttt ttctgatata
     6661 acaggtttaa atttagcttt ttataaaaat tgtggtcttt taccatatca tggaataatg
     6721 gctgccaatg tagctaaatg ggatgaattt acctataaat cattggtaaa tgctttagaa
     6781 tttaaagatg acttatattt agaaaatcca aaagaagaaa aaatttatac tgtatgtgaa
     6841 ggtaaagcag agggaataat aatgggagga aatttatcct taataattgc tacaatggga
     6901 acaaaatatg aaatagatgc aaaagataaa attttgttta ttgaggaaat tggagaacct
     6961 caatataaac tggatagaat gttaacacaa ctatattcct caggaaagct tgaggaatgt
     7021 aatggtataa tttttggaga ttttaaggat tgtattgaag aaaaagattt aatagaatta
     7081 ctaatagaat ttgcaaataa agtcaataaa ccttctatat ataatcttca atcaggacat
     7141 tgtataccaa tgataactat tcctttaggc agaatgtgcg aattagatgc tacagaaaaa
     7201 atcgttaaat taaaaaaata gtgtgtatat aaatataata gaaatatggt ataatcttat
     7261 acaagtaaaa atttagctag gaggatgaaa aatgtctgat gatagaataa tagactttaa
     7321 cgaactaaag aacaaagtta aagattctga tgtagataaa tttgagcaat acatatataa
     7381 cttatatttt tctgttatgg atggaaagat gtctatggct gagttttcaa gaaaaatatt
     7441 cgattatatg agagataaca acatatctca agaaaaattt atgaaaatac aaaagcaatt
     7501 tatggagaga tatggtatgg acactgaaga agtagaaaaa caacttagaa actttggaat
     7561 cgacccttct acagcaggtt ttatgagtaa taatacttcc agcaaagtat ctacagaaga
     7621 tttagagtca tttaagaaga gtgcaggatt ttatgaaaaa tatggagaaa aaatccaacc
     7681 aaagagttgt ataacaacat ttataaaaaa tgacttaaat gatattaatg taataatcga
     7741 ccaagaaaag ataatgcttt gtagtgatag aaaaataaat cttatggatt cagaactaaa
     7801 tgagttttta ttagaatata agaatatgtt taataagaaa ataaaggtag taatgtgcga
     7861 aactacaaat aagtatgact attaaaaagt gaatttaaat actgaagtag tatagattaa
     7921 tggtattatt taaaaataaa aatcgcaatt tattattcct tataaatttt tataaggagt
     7981 atctaaactg cgatttttta tattgtgaag attagtcata gtagataata tttttaaatt
     8041 gtaatcttat cattaaaact gtgtttttct attagaggtt ttattaaaaa tagttatctg
     8101 ataattgtat catatgtgat gcaaagtatt agagtattaa cgaatctctt ttaatacatt
     8161 aactccattt tgaatcatac tattaaaaaa tactaagttt aaaatctcag cagggtgaac
     8221 gttaggaata tcataagtat ctactaaatt cataggtaca actatatttt tttcaatatt
     8281 atgctgatta aaatatgatt ttagagttat tgcaaattga tatatacata tatctgtaca
     8341 atcgccaact ataattatat tgtctatatt atcaaaatcc aaattttcta atgcaaaaaa
     8401 tccattagta gaatttttag gaagaatttt taggttttct atcgatttaa gttcatcaac
     8461 caattcactt tctatatcat tttccaaaca gtgaacagga taactcaaaa gttctattga
     8521 cttagaagta tgagaatcag taaatgctat tactctattt aatttgtttg atattttttt
     8581 tgtaaacatt tcgattggct ttattaaaga ctctactcta ggagagtata atgcaccttg
     8641 ttttgcaaag ccattattaa catcaattat aaataaagcg gttttactca agtcataatt
     8701 ttctattttt tctattggta gattatctaa attactcttt aaagcttcaa gttcatttaa
     8761 taagttatcc atagttatac ctccaagata aatttaataa aattgtacat attaattata
     8821 taaaagtcaa ggagtgcaaa ctattgtgta attagcgata ttgttttgat tgtaaaagat
     8881 ttgaagcaat gtctggatag ttagtaaaaa taccatctac attgagtttt agacagtgta
     8941 gtatatcatc ctcttcattt acagtatata cattgacacc aagtaaattt ttatgacaaa
     9001 tttcaattag agattcattt actaaaacta gaggtggatg aaggtaacta ttacccagaa
     9061 atttagcaaa attgactaca ttgtttatag gtaaataaca caataatcct gtttttacac
     9121 tagggtctaa tttatgaaag ttttttaaac tattgctgtt gaaactagat ataagaactc
     9181 tattttttat attatacttt aaaataagtt ctaaaacatc tttttctata ttgggatagt
     9241 ttaccttatc agtttttagc tctatattta aaataaggtc tgtaggtgaa attagaatta
     9301 atacttcttc taaagttgga attttacatt ctttattgtt ttgatatcct tcaaaagatg
     9361 agtttagagt ctttaacttt tttaaagtaa agtcttttac aaaaccatgt ccattaaaag
     9421 ttctatcaac cttttcatca tgaataacta caatttttcc atccttagtt ctgtggacat
     9481 caagttctat tccatatata tctatctcta aacatttttt aaaagctaga atagtatttt
     9541 ctggatactt tccactaaac cctctatgag cataaatatt catttatgtg gctcctttca
     9601 ataaatataa cttatcttat aaaaatttaa ttaatataat atatcatatt ccaaaaggta
     9661 ttgt
//
LOCUS       DAMJVM010000118         9552 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.123, whole genome shotgun sequence.
ACCESSION   DAMJVM010000118 DAMJVM010000000
VERSION     DAMJVM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9552
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.123"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(88..585)
                     /locus_tag="PC523_003109"
     CDS             complement(88..585)
                     /locus_tag="PC523_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HDF2476606.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELKENESVTATDFVQGTTSWHGKLGG
                     LDRLDASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
     gene            complement(691..1053)
                     /locus_tag="PC523_003110"
     CDS             complement(691..1053)
                     /locus_tag="PC523_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2476607.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYKGYPLKVEYSLTPIRGKQILDALAIMQDIGIN
                     FMLDNGMEDILKEKGVLK"
     gene            complement(1071..1685)
                     /locus_tag="PC523_003111"
     CDS             complement(1071..1685)
                     /locus_tag="PC523_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2476608.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     LGNALRDISKKYPDLIKKELDSWDISNKKVEFVYKLASKRILAK"
     gene            2286..4421
                     /gene="rnr"
                     /locus_tag="PC523_003112"
     CDS             2286..4421
                     /gene="rnr"
                     /locus_tag="PC523_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDF2476609.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIISPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLS
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEVLARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYVYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
     gene            5560..6051
                     /locus_tag="PC523_003113"
     CDS             5560..6051
                     /locus_tag="PC523_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDF2476610.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDSNENSTINIDKA
                     WHAIHFTLTGCPFGGDEDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQEFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKRFIQEASDDGQAVIF
                     FIM"
     gene            6099..6251
                     /locus_tag="PC523_003114"
     CDS             6099..6251
                     /locus_tag="PC523_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2476611.1"
                     /translation="MKTIEKYIKEMIGESISEINSLWKTGESIKINYSKVSSERFEEI
                     IQRVTP"
     gene            6324..6735
                     /locus_tag="PC523_003115"
                     /pseudo
     CDS             6324..6735
                     /locus_tag="PC523_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(7010..7474)
                     /locus_tag="PC523_003116"
     CDS             complement(7010..7474)
                     /locus_tag="PC523_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2476612.1"
                     /translation="MNLNKVKFTEEHAIEVINWKYEGKYSIYNLPPWEEIKKKNFSLA
                     KEDRRKNFISFINDNKELIGFINLLDEGSSVFFGIGIKPEYCGMGIGKEIISLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYESQGFKIVETKRQKTYLGDGEFFVMRYQ"
     gene            complement(8486..9313)
                     /locus_tag="PC523_003117"
     CDS             complement(8486..9313)
                     /locus_tag="PC523_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HDF2476613.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPMMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGTMPTNDNMNNKVSKQK"
BASE COUNT         3682 a         1174 c         1331 g         3365 t
ORIGIN      
        1 aatatgtaaa aaatattcta ataacaatta agctaatcat caattaaatg taggcactta
       61 attaatgatt agctatagtc aatctaatta attagaataa cttcctttta gtgtaaatgt
      121 atctaaaaca tgtccttcaa tagctttgaa aaattcattc atatagaatc ctttttcaag
      181 gtcaaggatg gtatcaagtg caaatacatg tatttcatag acatgaggtc tgtctggagg
      241 tgccattcca ccatatacac tggcatccaa tctatcaaga cctcctagtt taccatgcca
      301 gctagtagtc ccttgtacaa aatcagtggc tgttacactt tcattttcct ttaactcatc
      361 ttttgttata tttacagcta accagtgtat ccatgcaaac ccacacacag gaacagaatc
      421 tttatcttct aaaaacacag caaaggatac tgtatttttt ggagcatctt caaactttag
      481 aggtagtgaa tatgtaggca taccaccttc attgaactga gttcctcttt taccatattt
      541 atcttctata acaccattta ctatacctgt actagttact ttcataataa tttacctccc
      601 aatataataa attaatagaa tatctctata attcaattat ataccagttg aaaataatgg
      661 taaattactt acttttttgt gggtatctat ttactttaaa acaccttttt ccttaagaat
      721 atcttccata ccattatcca acataaagtt aattcctata tcttgcatta tagccaaagc
      781 atccaatatt tgtttccctc gaataggtgt aagtgagtat tcaactttaa gtgggtaacc
      841 tttatatgtc tttttatcta caagaccaaa ttcaattaat tctttaagtt gttcaagtaa
      901 catcttttga ttaataccat taatttcctt ttcaagtttt gataaggaag ttggtcctat
      961 gcgtaatctc cataatataa tagatttcca tttgcctttt atcatatcat gtgttagttc
     1021 taatgggcaa gtatactcat cacgaagttt caaataagtt cacccccatt ttactttgcc
     1081 aagatacgtt ttgaagcaag cttatataca aactcaactt ttttatttga tatatcccat
     1141 gaatctaatt ctttttttat taaatctgga tattttttac ttatatctct aagagcatta
     1201 cctaaagact ttctcaagta ttcgctatca tcttctttta aactagagag tagtgatata
     1261 gcgacatcag gattagattt aaaatagtct ctactagtcc aaattctaag accttctgtc
     1321 acagctcttt ttatgtttgg actatcagaa ttaagccatt cttttataac aggaagtgcc
     1381 ttttcatatc ctacttcact gcaataataa tcaaatgatt tagctaatat ttcttgtact
     1441 ctccagtttt cgtctttact cacattgtct tttaaaaata acaaagcttc agaaatagtt
     1501 actgatataa aaccaagtat aaacacagct aattctctta cttgataata ttctgaccta
     1561 tataagtaga ttgatagata ataacacttt tttgagtcat tatttttcac aattttttta
     1621 gcaatatttt caatttcttt aaagccattt tctatagtaa taatttttgc aattacttct
     1681 tccaatttta tctcctccaa taagtagatt agtttaaatt ttatgtattt atttgtaaaa
     1741 aggtttgttt aatttagaca aaataaagac ttgtcaaaaa acgtttgcaa gtctttagta
     1801 ctagcataaa taaaaaataa taaaaaatca acctcattta acatataaat ttacttttat
     1861 tataagtttt atattataaa gttgtattta aaaaattaat tgagttatat aaattttaca
     1921 tgatttgagt atactaatag aataaattat tatatagtaa tacttatatg agatgaatat
     1981 tttaccaatc ttatttgata gtgtataatt aggtgataaa gagtaactta agcaattatg
     2041 ataaagaata atttaagtaa ttataatgat atttacaaat aatttataat ttacaaatca
     2101 tcataatttg tttgaaatta gtatgataaa gcttacatta aatcaaaaat ataaatatgt
     2161 aattttaact tgtaacaata ataaaaaata tagaaagcta tgtgataagt aaaacatgtt
     2221 ttgtctgaaa aatataaaag acaatctaaa taataatata atgaaaagta aaataggagg
     2281 gaaaaatgat accaggactt aaagaaagtt tgctaggatt aataaatgaa agtgcatata
     2341 atcctctaaa aaaagaagaa ttagcagaaa tatttgatat acattcatca gaaatgccaa
     2401 tgttttataa ttttttaaat gaattagaag aagatggtta tatctgcttt actaaaaaag
     2461 gaaaaataat atctccaaat caaatgggat actttgttgg aaaatttgta tcgcacaaaa
     2521 aaggttttgg atttgtagaa tctgatgttg aatacacaca ggatttattt atttcaagtg
     2581 acaatataaa tggtgctatg cataacgata gagttatggc agaaatagta gtacctgcaa
     2641 ctgaagaaaa gagagctgaa ggaagaatag taaaagtaat aaagagagaa ataactagaa
     2701 tagtaggtac tttccaatca agtaaaactt ttggatttgt aacaccagac aacaagaaat
     2761 ttactaagga catatatatc cctaaaaaac attttagtgg agcagtagat aatgataaag
     2821 tagtatgtga aattacagta tggccacaag aggatagaaa accagaaggt aaaattatag
     2881 agatactagg tcaaaaaggt gaaagaggcg tagaaataga ttctataata agagaacatg
     2941 gtcttccaga agaatttcca aagaaagtat tgcaagaagc agaagctgta gctgttgaga
     3001 ttcctcaaga agagataaaa agaagaagag atttaagaga tttaaatata tttactatag
     3061 atggagatga cgcaaaagac ttagatgatg ctatatcaat agaagtatta tctaatggaa
     3121 acttcaaact aggagttcac atagctgacg ttacacacta tgtaagagag aaaaataaac
     3181 ttgacaaaga agctttaaaa agagctacat cagtttattt agtagataaa gtaatcccaa
     3241 tgttgccaaa gactctttca aatggagtat gtagtttaaa tccatttgaa gataagttga
     3301 ccctttctat atttatggaa atagaccata aaggaaatgt ggttaaacat gaaatatgtg
     3361 aatctgtaat aaattcaaaa gctagaatga cctatacaga agtatctgat atattagaga
     3421 aagatgatga aaaacttaaa aagacttttg aacatgttgt tgaagatttt aaaaatgctg
     3481 aagttttagc taggattctt atgtcaagaa gggaaaagag aggggctata gactttaatt
     3541 tccctgaggc aaagataatt ttaaatggaa aaggtgaagt tgtagatata aaaccatatg
     3601 aaagaagaat atctaataag ataatagaag aatttatgct tataagtaat gagacaattg
     3661 ctgaacattt ttattggatg ggaataccat ttgtgtatag aatacatgag acaccatctt
     3721 tggaaaaaat ggaagaattg agtaagttta tatctacatt tggatatata ataaaaggtg
     3781 ataaagagga agtacatcca aaagcattac aaggtataat agagaaaata aagggcaaaa
     3841 aagaagaagg agctatcagt actataatgc ttcgttcttt aaaacaagct aagtactcac
     3901 cagaatgtag tgggcacttt ggattagcag ctaaattcta ctgtcacttt acttcaccaa
     3961 taagaagata tcctgacctt caaattcata gaataataaa agaatcttta aataataaaa
     4021 taagcggaaa aagacaagaa cagcttacta ctatagtgga ttatgcatct gttcaatcgt
     4081 ctgagaaaga aagaaaggca gagttagctg aaagggatgt acatgatttc tataaggctc
     4141 tttatatgaa ggataaagtt ggtcaagaat ttgagggtgt tgtatctagt gtaacttcat
     4201 ttggaatatt tgttgagtta gataatacta tagaaggctt gataagactt gcaaatatga
     4261 atgatgatta ctatgtttat gatgagagtt cttatagtgt tttaggagaa agaactaaga
     4321 agtcatataa gataggtgac cttgttaaga taaaggttga gagtgtgaat gtagatttta
     4381 aggaaattga ctttgagata gttgaaaaga tagaggagta gataaaacag ctaaaataag
     4441 agataacaaa ttgtggtgtt tgtgatatgg ataaatatat agtgcacaat actcgataaa
     4501 ataaattata aaaacaggta attaagaaat gtttacatgg aagtagccaa ctgaaagata
     4561 agaaattaag aggtatttaa tagaaataga taaatgagaa ataagaaact aagaagcatt
     4621 tagtagaaat agctagctga aaaataagaa attaagaagc atttaataga aatagctaac
     4681 tgagaaataa gaaattaaga aatatttaat agaaatggct aactgaaaaa taagaaatta
     4741 agaagcattt agtagaaata gctagctgaa aaataagaaa ttaagaagca tttaatagaa
     4801 atgtataact gaaaaataag caactaagaa acatttagta gaaatagcta gctgaaaaat
     4861 aagaaattaa gaagcattta atagaaatgt ataactgaaa aataagcaac taagaaacat
     4921 ttaatagaaa tagataaatg aaaagcaagt aattaagaac tatttaatgg aaacagataa
     4981 tttaaaatta cctaattaag aatagatata aaaaaatata ttttcgataa aaaataagtg
     5041 tatcatttaa aatttaagat acacttattt tttatttaag agaacttttc cttttatata
     5101 atttataaca agaggtttaa tatatttata attaatttca aaattattca tatctgccaa
     5161 attaaattct tacatatata attattggta tgcttattaa cacttcagaa atcattatag
     5221 tttaaatttt gagtttattg ttattactta tttaatttga gcacattatt tatttttatt
     5281 tgcaactaat aaatagagta tgattttata atacagtttc ttaaattttt tttaaaagta
     5341 atagttctta tttgtagcca atattgctta tagaacttat tgattcattt aatttttaga
     5401 aaaaataata atacttacac aataaacaca gaattttata tagataatat aggtatttaa
     5461 atttaaggtg aaatgatact tttcttaata tattttattg taaataagct tgattataag
     5521 ttaaaattaa aaaaaacact acttaggagg aatgtaatta tggggatgat aggatgctat
     5581 acaaaaataa gtgaggagaa tgtttttaaa ttacagcaag cagaagaaaa tttacaagat
     5641 ttcgtttttg aagattccaa cgaaaatagt actattaata ttgataaagc atggcatgcc
     5701 attcacttta ctttgacagg ttgtcctttt ggtggagatg aagataatat atttagcaaa
     5761 ctagtgctga gtggaaatat acttatggaa attgatggag aatttcctgt catgttaatt
     5821 acagctaatg atgtaaaaaa actatcaaaa gcaatgaata gtttggaaga acaagagttt
     5881 agaaaaagat ttaatattaa tgagatgtta gaaaacaata tttatcctgt tatgaatgat
     5941 gagagtgaag atgatttttt tgaatatgta tgggcaaatc taattgagct taaaaggttt
     6001 atacaagaag catctgatga tggacaagct gtaatatttt ttataatgtg ataatcacta
     6061 aaaaattgtg aatattagtg tcatcacata tcttagatat gaagaccata gaaaaatata
     6121 taaaagaaat gattggtgaa agtattagcg aaataaattc actttggaaa actggtgaaa
     6181 gtataaaaat aaattattct aaagtatctt ctgaaagatt tgaagaaata attcaaagag
     6241 taacacctta ggaggagtgt ctacatattt tttgacaagt tacaaatatt tggagcagtt
     6301 tcagctacct ataatgaatc tatgatgata tagtatcctc aaaatcatta tctcaagtta
     6361 ttgtacaatt aattaggata ctaatgtcaa ttataagtat agcggtatta atcataggtt
     6421 ttgtaaatat tttaatatat cttggaggcg attttttagt agttgagtat atttggaaat
     6481 ttcgcattgg ctatgtctat aggaagttta ggaatgtttt tctcaaattt atttaataat
     6541 acaatcatag gttatatggt atctatggga tatttaattt taaatatgat gactaaaggc
     6601 aagtatgttg gaaatttttt tataatgtct atgagtcaaa gtagttttga tgagaaatat
     6661 tagttgatac ttggaagtat tgtgttgata attgaatcta tcttaataaa accaataaaa
     6721 aggaagatta gataataaaa tgaggcttag ataaaataaa ggtaatacaa acaaaaagta
     6781 catttatgaa atatgaattt atttttacat acacaaacct acttataatc tctttatgca
     6841 tgtagataga tttatatatc agtagtataa aatacatatg tataccgaat tttatcaaaa
     6901 gttcccaaat ttatatttca aagaccccaa agacacttat tgctatgacc tacgtacatg
     6961 tattttttca ttttaatttt tatattagca atatgactag tgtcgtaaac tattgatacc
     7021 tcataacaaa aaattctcca tcccctaaat atgttttctg tctcttagtc tcaactattt
     7081 taaatccttg agactcataa cattttacag ctctcatgtt ccaggttctt acatttaata
     7141 caattggttt agtactatat ttatttctac attcttgtaa tcctaggcta attatttctt
     7201 taccaattcc cattccgcaa tactctggtt ttataccaat accaaagaat acagagcttc
     7261 cttcatctaa taagtttata aaacctatca attctttatt gtcgtttata aaacttataa
     7321 aattttttct tctatcttct tttgctagtg aaaaattctt ctttttaatt tcctcccatg
     7381 gaggtagatt gtatattgaa tatttacctt catatttcca attaataact tcaatagcat
     7441 gttcttcagt aaatttaact ttatttaaat tcatatgtcc tcctattaaa atatttaagg
     7501 taattaatgg tattttaaaa ttttagaaaa taagtaaaac tatttatatt tatccatgaa
     7561 atctttaaaa attttactaa caattcttgt accctgtatt ttttattgga ttcacagaag
     7621 actataaact aattttgaat taccttctaa ttcaaaaacc tcacaatata tgtaacttct
     7681 tagcttgatt aaattctgtt aaatctgttt cttatagatt tcttatagtt gtataatcaa
     7741 catcaatata tttatgtgta ttagttaatt tattgacttt aggaacttat tattaatatg
     7801 ttatatttgc ttcaatactc caatagtagg gtaaattgta ggcaaattat caaatgtata
     7861 tctatccatg cttaaacttt agagatacaa taactactaa attagtattc tagtagttat
     7921 aaaaaagaat ctttaaaaat attaatttaa cttatatttt ttaataaatt ttctaaaatt
     7981 taatcagtta atttaattat agctctaaat gatattttct acagatagaa tttcaaaaag
     8041 atgtgtataa gaacaagttc acttaaattt ttaaaatcaa ataaagttga atctcttgat
     8101 taccttgatt attttatgaa cttaatatag atgtaaactt tactattaaa aaaatacgag
     8161 attttagata agttaatgct aatttaaata ataagataag aaatataata tgacataaga
     8221 gctaggaata agataaatat aatcaaagaa taaggcatga gaataattac caagagaaat
     8281 cgttacttta atatgtttat gaatgttaga ctatgttcct aaattgaatt ttatatatca
     8341 aatataataa cttacagaat gtggaaattt ttatttaaag aatacacata aacatttcca
     8401 cactctgatt ttataataat aatctaataa aatttatagt gactttataa actcaataat
     8461 gatttaataa atatttataa aacaactatt tttgcttaga aactttatta ttcatattat
     8521 cattagtagg cattgttcca aaacttagtt tttcatacat tacttgagtt ggagctagcc
     8581 aaactgtacc attaccagta aatgtattta agaaaccttc accatttaaa gcagaaccaa
     8641 ttaaagattt atctgacttt gtaacagaaa aattaacacc agatgttctt gctattgcaa
     8701 agttaccatc tacctttaat tcttcaccat ttttcaattc atactctaca atttcacttt
     8761 gaggaacaac acattccaga acgattacac cagtaccact taattctatt tggaagaagc
     8821 cttcaccacc aagtaaagca gatgagaagt tcttttgtgt aaatgctttt acttctattg
     8881 tatcagagca acaatagaat aatcctttat caacaattat ggaatcattg tcaagactca
     8941 taaataaata gtgcctaaaa gaaggttcca aataaacttc tccagtaccc ttgtatacag
     9001 gtttcatcat tgattcatta gtaagtgctc ctgatattcc ttttttgaaa agacctccaa
     9061 caccaccaat cttagtttca gaagatatat ttcctttata atagtataat gcaccagctt
     9121 ctgtttttat tgatgaatta tttaatttta tcttaatttg tctagctttc aaaccagctt
     9181 ctctagcaaa atataatccc attgccatca ttgggctaga agctccctca agtttttcaa
     9241 attctaatat ctcaaattcg cttttgccat ctgatgcatt ttctaaaatt tttacttttt
     9301 tgcttaaatc catacaaatt tctcccgact atgttaagat ttatctctaa aatattataa
     9361 gatataatta tatattacat tttttttgaa aaaatggcta ctttatcatc aaatttttaa
     9421 atatttttaa taagtttttg aaatgtttag atttaagtta aattgtttaa gaacatatac
     9481 ttgtttcaat aggtattttg agtattattt attgcttttg ggtattatat tttatgggta
     9541 tgttaaaatt at
//
LOCUS       DAMJVM010000119         9535 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.124, whole genome shotgun sequence.
ACCESSION   DAMJVM010000119 DAMJVM010000000
VERSION     DAMJVM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9535
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.124"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(137..790)
                     /locus_tag="PC523_003118"
     CDS             complement(137..790)
                     /locus_tag="PC523_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2476614.1"
                     /translation="MEERLMKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIG
                     VSRTPAREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQN
                     LTESDLVKMEELIEMMAVSIKYKNYSSYLKLSNEFHDIYITKSNNQVLVNLLNSLRYN
                     FMSKSYTSDNEDELYKMLTYSNNQHIEVVKFMRENDLENIERVLREHWKTIPIAEMK"
     gene            complement(1021..2886)
                     /locus_tag="PC523_003119"
     CDS             complement(1021..2886)
                     /locus_tag="PC523_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HDF2476615.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELNICVKVNEGYNVKSIFL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDIPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELINKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDK
                     DSVINKWINMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFKSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYKDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRILILVN
                     RSENVESISLCMDSSFIEEIPIKYSLKNVNSTIQIENNELKVDLESKSFRIFRVN"
     gene            complement(2891..5311)
                     /locus_tag="PC523_003120"
     CDS             complement(2891..5311)
                     /locus_tag="PC523_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HDF2476616.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLDIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFINGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRVKRMSIIDYDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKRDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNIDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(5325..6767)
                     /gene="glgA"
                     /locus_tag="PC523_003121"
     CDS             complement(5325..6767)
                     /gene="glgA"
                     /locus_tag="PC523_003121"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDF2476617.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTNAVLESISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQTYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDASIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(6796..7926)
                     /gene="glgD"
                     /locus_tag="PC523_003122"
     CDS             complement(6796..7926)
                     /gene="glgD"
                     /locus_tag="PC523_003122"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HDF2476618.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(7928..9088)
                     /locus_tag="PC523_003123"
     CDS             complement(7928..9088)
                     /locus_tag="PC523_003123"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDF2476619.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
BASE COUNT         3017 a         1750 c          924 g         3844 t
ORIGIN      
        1 atatactaca tattgtagtg tcttagagct tttatttgta ttttaaatta aatttattct
       61 tgtttttcct ttaataatta atagtaaaac aaaaaaaata taaaaaaaat aaagagaaac
      121 tttataaaag cttctcttat ttcatctctg ctataggtat cgttttccaa tgttctctca
      181 aaactctttc aatattttct aagtcattct ctctcataaa ttttactact tcaatatgtt
      241 gattattgct gtatgtcaac attttatata actcatcttc attatcacta gtatatgact
      301 ttgacataaa attgtatctt aaagagttca atagatttac taacacctga ttatttgatt
      361 tagttatgta tatatcgtga aattcattgc ttaattttaa atagctactg taatttttat
      421 actttataga gaccgccatc atttctatta attcttccat tttcactagg tctgattcag
      481 ttaaattttg taatgctgaa gaaccggcta acgcatccaa tactccaatt acagaatagg
      541 tgtctaattt ctccttttct tgaaattctt ttactacaaa tcccttatta ggtatttttt
      601 ctagtaaatt ttcacctgct aacctagtta atgcttctct cgctggtgtt ctactaactc
      661 cgatagattt acaaatctca gattcagtaa ttctctgatt agcttcatac tctccatttt
      721 ggattttctt tgatatatag tcatatactt gagttgtaag tggatctacc ttcataagac
      781 gttcctccat attaagatta taaaagttaa atctccctac aactttggta gagagttcag
      841 ttttatctta ctaatttata taatatcaca attatgatat tttttatact tctttgtaat
      901 attttgaaat actaaaattc ttatgataat ttatacaaaa aaatgtagaa aaagttttga
      961 taaaacaaaa ctctttccac attcccctta ttttctattt atttttctaa acaaatatat
     1021 ttaatttact ctaaaaattc taaatgattt agactctaaa tccactttca actcattatt
     1081 ttcaatttgt atagtactat ttacattttt aagactatac tttataggta tttcctcaat
     1141 aaaactacta tccatacata atgatatact ttccacattt tcacttctat ttactaaaat
     1201 taatattcta tcattatttt cattaactct aataaaggaa aatacagaat cattattagt
     1261 atatataaat tctgtatttc cagaacttaa aagtttattc ttatttctaa tttttataac
     1321 atctttataa aaattaagca tatcttcatc ttcatttttc catggatatg ttcttctatt
     1381 atctgggtca acacctccac aaagacctgc ttcatctcca tagtatatat atggtactcc
     1441 ttcaaatgtc atctgtatag ctacagcaag cttcactaaa tctacatcat catttaattc
     1501 tgttttagct cttgtaacat catgtgttcc aattaaattt aaattagact taaatgattc
     1561 ctttggataa ttttctttaa tctgcatata tctattacaa agttcgtatg aattaatttc
     1621 tccttttaag aaagaaaaca tgttatttct aaaaggataa cccataacag aatctaattc
     1681 ttcacctaat aaataacttc ttctctgacc atagctaatc ttatttgatg catcttccca
     1741 tacttcacct ataagaattg agtcatcatc cgcctttttt aattcctttt ttagttctct
     1801 tataaactta gttggtagtt cgtctgcaac atctagtctc cagcccttaa tacccatatt
     1861 tatccattta tttataacgc tatccttatc atatattata taatccatgt agctatgttc
     1921 taattcattt atatttggca atgtcttaac atcccaccaa cttttatact tttggggaaa
     1981 ttcttcaaac atataccatg aataatatgg agattctttt gattgatatg ctcctaaact
     2041 attatagttt ccatacatat taaaatattt actatctgct ccagtatgac taaaaactcc
     2101 atctaatact attgatatac ctttttcttt tgctttatta ataagttctt taaatgtatc
     2161 ttcatcccca aacattgggt caattttttt ataatcacct gtgtcatact tatgattact
     2221 agaagcttca aatatagggc ttagatatag tatacttaca cctaattttt ttaaatatcc
     2281 aagtttattg attatccctc ttaaatttcc accatggaaa tcccacctta taacatcccc
     2341 ttgactatcc tttatgtaca taggtatatc ttcccaattt ccatatatga aactattttt
     2401 tttaggatta tcaaccttac cactacgatt cccattatta aatctatcta caaatatatg
     2461 ataaagtatt ccttctttgt accacgttgg tactttaaaa tctttgtaaa cagtgatttg
     2521 atatttatta atatcactat aattatattc acaagagtat ccgttatctc tgttttttcc
     2581 ataaaataaa gtctttatct gaccatccat ttcaacatca actttaaagt aataaaaata
     2641 aacacctgtc ccatcaaact tttcaatctt accacaaaag cactttccaa gtttatcatt
     2701 atctaattct tcatttaaag ttattgttct catttcttct tctctattta tttctaggaa
     2761 aatagacttg acattgtatc cctcatttac tttaacacaa atgttcaatt cctcatcaaa
     2821 ttttaaagct ccaaacggag ccttaaaatt tttatcccaa gaattatatt ctattacact
     2881 ttgcatttaa tcacaccctt ttaaaccaaa tgtcttttgc atactcagaa attgttctat
     2941 cagaagaaaa aaatccagag ttagctatgt taactaaaga cattttattc catttttctt
     3001 tatctctata taacctgttt atatctgctt gagcttgacc ataattttca aaatctctca
     3061 atacaaaata ctcatcattg taagtagtga gtgagttata aatacttctt ccttcttcgc
     3121 caagattagg aataaatcca tttattaaat catctactac cctttttata tccatattag
     3181 aatgatatag gtcaagtgaa gaatatccac catatttatt ataatttagt acttgttctg
     3241 cactaagtcc aaataaaaac atattttctt taccaacctg ttcacatatc tctacatttg
     3301 caccatcaag tgtagccaaa gttatagcac cattcatcat aaacttcata ttacttgttc
     3361 cagatgcttc ctttgttgta gttgaaattt gctcactaac atttgctgct ggtattatta
     3421 tttctgctaa tgatactcca taattctcta aaaatactac ttttaacttg tcctttactc
     3481 taacatcatt atttatagta cttgcaacag aatttataaa tttaattata catttagcca
     3541 aataatatcc tggtgctgct tttgcaccaa atataaaagt tcttgggtca agatttaaat
     3601 taggattatc aagcaattca tgatacatat gaagtacatt aaatatattc agtaactgtc
     3661 ttttatatgc atgcaatctc ttaacttgaa catcaaatat tgaacttggg tctatattta
     3721 aatcgtattt atcttttata aactttgcta gatttgcttt attattgtat tttatatcat
     3781 caagtctttg taagacagaa gaatcatttt taaatttttc aatatttttt aattgtaagg
     3841 tatccctctt ccatgaatca ccaattaaat ctgtaattaa attgctaagt tgaggatttg
     3901 aacttataag ccatcgtcta tgtgcaattc cattagtctt attattaaat ttatttggtt
     3961 catcttggta gaaatctttt aatacctctg tttcaagaat atgagtatga agcttagcaa
     4021 ctccatttac actatgactt gtcacaatac ataaatttgc catattgata ttgtcatagt
     4081 ctattatact cattcttttt actctatctt ggtcatatcc tttattattt aattcctcta
     4141 catatcttct atttatttct tctattatca tataaattct tggtaatagt tctttcatca
     4201 tacttacagg ccatttttcc attgcttctg acattatagt atgattagta taagacactg
     4261 tttttttagt tatttcccat gcttcatccc aagataaatt ttcttcatca agcaaaattc
     4321 tcataagttc tggtatgcat aaggtaggat gtgtatcatt tatatgaatt gctaccttat
     4381 cgaataaatt attgatattt aatttgttct ttttatattt tcttattata tcttgtatcc
     4441 ctgcacttac aaaaaagtat tcttgtttca acctcaacaa cttacctgca taatttgaat
     4501 catcaggata taacacctgt gatatttcct cagtatagta cttatgtttt aaagcctctt
     4561 catagctacc agagtaattt agagcatttg aggtaagctc accaaaatct ctctttggta
     4621 tttcactctt aaacaatctt aaagtattta tacattggtt ttggtatccg attattggta
     4681 tatcataagg cattgccatt acaggaaggt agtttttatg aattactttt aaatgactac
     4741 cttccttgat taattctacc tcaccaccaa acttcaccat tgttgcttca tttggtctta
     4801 cagtttccca tgcatatcgt ccttctgtaa gccagttatc aggcacttct acctgataac
     4861 catttataaa tttttgttca aacagaccgt atttatatct tattccatat ccttgaccac
     4921 ttatgttcaa agacgccata gaatctaaaa agcaagctgc taatctacca agcccaccat
     4981 ttcctaaggc tgggtctact tcagcttcta ttaaatcatc caagttgatt cctaactctg
     5041 acaaaccttc tctaacttct tcttcaatat ctaaatttaa taaattagat tttaattgtc
     5101 tccctattaa aaattctaaa ctaaaataat aaacctcttt tttcttgtct aactttgtag
     5161 caacccattt ttttgctatt tcatctttca atagactaca taatacattt aacaattgtt
     5221 catcagtagc ttccttgata gattgtgcat ataagctata catttttact tcaaattttt
     5281 gcttgaattt ctttttgctt attgttacca aaaaaaccac tcctttaatc acatatgtct
     5341 ctatatgttt ctatatactc cttagctgat tttttccaac tattatctgt tttcattgca
     5401 ttttcaacta atttaatcca tttttctttg tccttaaaca ctttgattgc atttttcaaa
     5461 cagaaaaaca tctcatgagc attgtaattt ttaaaactaa atccattgcc ttctccagta
     5521 aatttattat aaggtgttac tgtatcccta agccctccag tctccctaac tattggtaaa
     5581 gaaccatatc tcatagctaa catttgacct ataccacaag gctcaaataa tgatggcatt
     5641 aagaacatat cacttgcagc atatatctgt tgagccaatg atgcattgaa agtaattcta
     5701 gctgatactt tatctgaaaa attagaatcg taatagttga acattgattg atattgatgt
     5761 tctccagttc ctaaaacgac aatctgtaaa tcttctctca taagttctgg catcatgtat
     5821 gctattaaat caaatccttt ttgagaaact aatctcgaaa ctatacctat caacggaata
     5881 gatgcatctt ttttaagtcc caatatatct tgtaatctca atttattttc tacttttttg
     5941 tcaattgagt ctacatcata atgaacaaat atatctttat cttttgcagg gtcatttaag
     6001 tcataatcta taccatttaa tatacctttt aattttcctg actcttttct aagtaatcca
     6061 tctaattgtt caccataaaa agatgtttgt atttcatttg cataagtagg actaacagtt
     6121 ataattttat ctgcaaaatt tataccagct ttcataaaat ttattgagcc ataatactct
     6181 atatctccat tatcaaagta tgtttgtggt aagtccaata tatcatataa aacatgctta
     6241 tcaaatacac cttgatattg gagattatgt atagtataca tagtttttat atttttatac
     6301 ccctcaaggg ttgcatatct ttcttttaga attaatggta acatccctgt atgccaatca
     6361 tttatatgta ttacatctgg ataaaaatca attctactta tagattctaa aactgcattt
     6421 gtaaagaaag tatatctttc tgcatcatct ccatatccat agatgctctt tctttcatct
     6481 tctcttctaa aataaaactc attatctata aagtaaaatt ttacaccatc aagttccatt
     6541 tccaaaagac cacagtattg atttctccat ccaacacgta cactaaacac agctatttct
     6601 tttagttgat cttttaaata acttggtata gttgaatatt tgggcattat tacccttaca
     6661 tctacacctt ctttttttaa ttctttaggc aatgcataag aaacatctcc taatcctcca
     6721 gtttttgcaa agggccaaca ctctgctgtt acataaaaaa ctttcattag tcattccccc
     6781 ttctaaaaaa tttatttata tatttgtatt cttttccact acaagaggat atttttcatc
     6841 tcctcttaat tctttcccat ttgatacact cgtattttta tctaatattg cattatataa
     6901 ttttgcattt gactttatta cacaattttg cattactatt gagttctcta taactgttcc
     6961 tttttcaaca tttacctttc taaatatgat actatctttt acagtccctt caattaaaca
     7021 accagatgct ataaagctgt tttctacttt agcactatct gtataaattg taggtgattc
     7081 attcttttct tttgtaaata tctttctttc agaatataat aattcatcag caacttctaa
     7141 atttaataaa tctttgctca tttcaaaata tgattcaaca gaatttacac attttagata
     7201 tcctgtatat tcataagctc ctattttcat gttttctgct tcttcaataa taaattcttc
     7261 taaataacaa taattgcctt tatttacaat gttatatata cattcaacaa aatcttcacg
     7321 tttcataata tacgtttcca tacatatatt tgctctaggg aatttaccca aattatttcc
     7381 tacatttatc actctattgt tactatctaa atttagagtt aagttaccag caaaatcttt
     7441 atttgcatta tttacatttt tatatattat agttatatca ttatgagatt ttttgtggta
     7501 ttccaatgct tgagaataat ttaaattaca tagcatatag cttggagcta ttaaaatata
     7561 ttcttcttca ctcttttcta tataatctag attagctaag atagtatata tatcaccttc
     7621 tctatgtgag taagtatttc tatacttagt gttttctgga ctaaaaattg ataggccacc
     7681 tttctttcta cttaaatccc aatctttacc actacctata tggtctgtca atgatctgta
     7741 tttttccttt gcgaatattc caaccttagt gattcctgaa tttatcatat tagataaagc
     7801 gaagtctatt attctatatc ttccagctat tggtgtagat gctattggtc ttccatagtt
     7861 aagtttgttt attgctgggt ctcctttttt gtttaaattt attatcccta aacattcatt
     7921 tctcatctta aagacctcct tctattaata cgcatctagg ttctatattt tcatactcag
     7981 aaactacatt tatttcatta tttacattct ttatctgaac attatcattt atagttgcac
     8041 actcacctat catagctttt tctatagtta cattttcacc tattacaaca tttggcatta
     8101 ttactgagtc aattacttta cttcctttac caacaacaac tccatgtgat agtacagaat
     8161 tacctacttc acctaaaatt ctacaaccat cagctatcat tgatctatga acatttgcat
     8221 ttttacctat atattgtgga ggcattgcca tagtatttgt atatatcttc catttagggt
     8281 ctgctaaatc aagtgtttct ggagatttta ttatatccat attagcatcc cataagcttt
     8341 gtatagttcc tacatctctc cagtatcctt taaatggata ggcatacatt ccaacattat
     8401 cttctaacat tttaggtatt aaattttttc caaaatcatc ataatttatt tccggatttt
     8461 tttctgcttc cttaaaataa tttctcaaca ttttccaatc aaatatgtat actcccatag
     8521 atgccaaatt actcttaggt tcacttggtt tttcttcaaa ttcatagata cttgaatctt
     8581 catttgtatt cattatccca aatcttgaag cttcatccca aggaacttca attactgcta
     8641 ttgtagcttt agatcctttt tccttatgaa attttaacat tttactataa tccatcttat
     8701 atatgtggtc accagataat atcaatacat attctggatt ataggtatct acaaagtcca
     8761 tattttgata tatcgcatgt gctgtaccat tataccaatt tccttctttt tcattcataa
     8821 atggttgtaa aacatagacc ccaccattta ttctgtctaa atcccaatga cttcccatac
     8881 ctatatgtga atttaatatc aacggtctat attgagttaa aacacctaca gtatcaatac
     8941 cagagtttga acaattactt agcacaaaat ctataattct atacttacct ccaaatgata
     9001 cagctggttt agcaattctt tttgtaaaga cgcctaatct tgaaccttgc cctcctgcca
     9061 aaatcatagc taacatctct tttttcattt aatgacactc ccctcatcta gtaattagta
     9121 tttataattg catttttact tttataaact ttatcagtca ataaatgttg tgtataaatg
     9181 ttgcttaaat aataccttaa taaaaataaa ataatatcaa ctatacaatt gatacaatgt
     9241 ataccccata atgcacttgc cgacgtatac attattttcc ataatcacta tagtattaat
     9301 taatattcaa ttatcagtta aaaaataata caaattttat tttataaata taaaaacatt
     9361 ttttataaat ctcttgtgcc atacaaacat acttgttttt taatttgtta aattcataat
     9421 agcaaaatac aatctaacac ttgttaaata attatccaaa taaaatcaag taattattaa
     9481 tcaattttat ttttaattga tacttctttg tctatcattt aaacaagaat atata
//
LOCUS       DAMJVM010000120         9410 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.125, whole genome shotgun sequence.
ACCESSION   DAMJVM010000120 DAMJVM010000000
VERSION     DAMJVM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9410
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.125"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            73..573
                     /locus_tag="PC523_003124"
     CDS             73..573
                     /locus_tag="PC523_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2476620.1"
                     /translation="MIEGIKFIKAEKKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNTITKNLPQLFVIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIINLSKEYGYEKIELDVLKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(814..1593)
                     /locus_tag="PC523_003125"
     CDS             complement(814..1593)
                     /locus_tag="PC523_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476621.1"
                     /translation="MNKNILNNSFPEVPESFHNSLNKTLNSLPEREENYKMINNKTHK
                     LPFGKGLVATLAITLILGTTALAVGQISSLVAKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNEEALDKEDNVLGELKALSFEYKNGNDEISLKTANNVLGNS
                     SDNLKVVDTYKDINISYKAFAQKFVPEGYKMTEQDKVDEKSGKYTFSVGPNNESGKVE
                     VNEFKFLTWKQDGVYYSFTVQDSNLSMNELVKMVHEVIDAK"
     gene            complement(1590..2069)
                     /locus_tag="PC523_003126"
     CDS             complement(1590..2069)
                     /locus_tag="PC523_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDF2476622.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKFLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            complement(2598..4001)
                     /locus_tag="PC523_003127"
     CDS             complement(2598..4001)
                     /locus_tag="PC523_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2476623.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEVCYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     misc_feature    4706..4848
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            4870..5379
                     /locus_tag="PC523_003128"
     CDS             4870..5379
                     /locus_tag="PC523_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDF2476624.1"
                     /translation="MSKELAINEQIKDKEIRVLSQTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            5407..5601
                     /gene="rpmI"
                     /locus_tag="PC523_003129"
     CDS             5407..5601
                     /gene="rpmI"
                     /locus_tag="PC523_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDF2476625.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAYKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            5657..6013
                     /gene="rplT"
                     /locus_tag="PC523_003130"
     CDS             5657..6013
                     /gene="rplT"
                     /locus_tag="PC523_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDF2476626.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGIEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            6314..6859
                     /locus_tag="PC523_003131"
     CDS             6314..6859
                     /locus_tag="PC523_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476627.1"
                     /translation="MNDKLMDCFVNPIKCKLLLEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSVFSVCPVYASIDEIVEASKKIASILEPLKNNSHE
                     EGRRMHSIGVIITPPKEDEGK"
     gene            7044..7961
                     /locus_tag="PC523_003132"
     CDS             7044..7961
                     /locus_tag="PC523_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HDF2476628.1"
                     /translation="MRLITRSDFDGLVCGALLKEAGIIDSWKFAHPKDLQDGLVEVTE
                     DDCLANVPFVEGCGLWFDHHSSEFERNKLEGRYKGESRIAPSCARIIYEYYGGNERFS
                     HFNEMLEAVDKVDSGNLTIEEVQNPSGWILIGYLMDPRTGLGRWRNFTISNYQLMEKL
                     IDACRTLSTKEILELPDVKERIEVYFEQTNKFKEMIAKYTRVEGDVIISDLRGVEPIY
                     TGNRFMIYSMYPEQNISAWIVNGKGGKGCSVAVGYSILNRTSSVDVGSLMLKYGGGGH
                     KAVGTCQFNDEQIDELLPKLLDELVNTKN"
     gene            8349..9377
                     /locus_tag="PC523_003133"
                     /pseudo
     CDS             8349..9377
                     /locus_tag="PC523_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
BASE COUNT         3539 a         1097 c         1538 g         3236 t
ORIGIN      
        1 atagcattat aaattagaat catataaaat aagttaacta caatcataaa ttaaaaaagg
       61 ggaaatgtta agttgataga aggcataaaa tttataaaag cagaaaaaaa gtatatttat
      121 tcgtattggc aaacttttga taaaatagca aaagagagga aatatctagc tatggatgag
      181 gcctttcctt ttgaagaaac agttgaattt ataaaaaata ctataactaa aaacttacca
      241 caactgtttg taattgattt agaatcagat aactgtattg gttggtgcga cgttttacct
      301 aaaactgaaa aagttggtta tttaggcatg ggtattctta aagagtatag ggaaaaaggc
      361 attggaagta gtcttttaaa acaaattatt aatttatcaa aagaatatgg atatgaaaaa
      421 attgagcttg atgtgttgaa aagtaatagt agagctattc atgtttataa atccttaggg
      481 tttgtagagg taaatacaat atctagtggt tttacatgga atgatagacc tgtaaaagaa
      541 gaggtaattc aaatggagct tacacttata taaaataaag ttaaacccct taaaattcca
      601 tatacactta tataaaataa aattaaagcc cttaaagttc catataaatt taagggcttt
      661 ttaatagtgc ttcttttgaa agtagttaaa tatctaaaat tttatatatt aaatttaaag
      721 aaaatataaa gatggaagat acaaccatgt ttgctaataa aaaaattaaa gtcgtaaata
      781 ataaactcat aaacaactta ttattactta tgtttatttt gcatctatta cttcatgaac
      841 cattttaact aattcattca tagataaatt tgagtcttga actgtaaaag aatagtaaac
      901 tccatcttgt ttccatgtca aaaacttaaa ttcattaact tcaactttac ctgattcatt
      961 atttggacct acactaaatg tgtatttacc tgatttttca tcaactttat cctgttctgt
     1021 cattttataa ccttctggta caaacttttg tgcaaatgct ttataagaaa tattaatatc
     1081 tttgtaagta tctacaactt ttagattgtc agaactattt ccaagtacat tatttgctgt
     1141 tttaagtgat atttcatcat taccattttt atactcaaaa ctaagtgctt ttaattctcc
     1201 taaaacatta tcttctttgt caagtgcttc ttcatttaca gtatatgcac ctttaaattt
     1261 atatccatta ctaaattcag aaactatatt aggggtaaag ttaaagtctt tttttacttg
     1321 ttctgtagtt gggatagatg tataggtagc tttagtactt gattttgcaa ctagactaga
     1381 tatctgccct actgcaagag ctgttgtgcc caatattaaa gtaatggcca aagttgcaac
     1441 taagcctttt ccaaatggta atttatgtgt tttattatta atcattttat aattttcctc
     1501 cctttctggt aaactattta gtgttttatt taaactatta tgaaaacttt ctggaacttc
     1561 tggaaacgaa ttatttaata tatttttatt catatgatta cctccgtgtt ttctaattta
     1621 gtttttaata atcttcttcc tcttgataat ctacttttta cagttccttg aggtatatct
     1681 aatatttcct taacttcatc tactgaataa ccttcaatat agtaaaggac tattggaatt
     1741 ttaatttttt taggtagact ttgaatagca gtatacaaat cactataatc atctttttca
     1801 tcaatttttt tgtttttgaa gacgtcttca aatgatagaa attttaatcg tttacgtctt
     1861 aatgaattac attcatttat gactattcta atcaaccagg ttttaaagta ttgttcttct
     1921 ttaagagaat ccaacttttc atatgctttt aaaatagcat tgttgacggc atcttcacag
     1981 tcttggtcgt ttcctagaat agacttagat actcgataca aagtctgttc tgattcaagt
     2041 atattgtttg taaatgtagt tttatccaat tgagttccta cctttctatg caaatcgtag
     2101 cgctactttt ttgcctttca cttattagat aaaattttac tatagatggt tcactttctt
     2161 tataattatt tgataaattt aagtttaaaa gttttgtcta ttctatacaa atattctata
     2221 atatattttt ttacagtcat atataaagcc atgtatatat gaaactttag aagctattta
     2281 tatatttagt tttatagtaa gtgtttatat tgagtaatta gaaaataata agtatttatg
     2341 ttgagtagtt ggaaaataaa taatattttt gaattattta attcatgtat agatttaaaa
     2401 taagttcttt aaatagcgac tttaaataga tataagtcat aaattaaata gacaaaatta
     2461 aaagatggaa gtatatcaaa atgaattttt tagttcataa ggcgtttttt gtatgaaatc
     2521 aaataagttt ttataatttt aacaataaaa agaggcttat ctctattttg agatagcctc
     2581 atatttttaa aactatacta gtttacagct tcctttccta tcaaatcttt taactcttca
     2641 tgagtcatag tttctttttc aaataagaat ttagaaacag aatgtaaatc ttcaagatta
     2701 tcctttaaca tctctaaagt ctctttataa caaacttcaa ctatacgatt tgcttccttt
     2761 tgaactttat cacataagaa agtcttatca tttccatcaa tagtcataaa ccctaaatta
     2821 ctcataccgt attcacaaac catttgatta gctatttcag tgactttctt taagtcgtcc
     2881 tttgcaccag tagatttatg attaaatatt aattcttctg aagcctttcc agcaagtaat
     2941 atttttattt ttccaatcaa ttcatcctta gtataaatat atctatcttc atcaggtaat
     3001 tgaagaacat aacctaatgc ttgtcctcta ggaactatag atattttttg tataggacaa
     3061 attcctacta tatcacttac aagagcatgt ccagcttcat gatacgctac tatttttttc
     3121 tctttttcaa ccaatgcaga gtttttagat tctagccctg caataactct ttcaagtgcc
     3181 ttgtcaaaat gttctgatgt aatacactca ctgtcatctc taactgcaaa tatagctgct
     3241 tcatttgcta tatttgaaag atgagcccca ttaaaaccat gagttttttt agctaaaagt
     3301 tctagattta cagatttgtc tataggtttg tcatctgtat gtacctttag aatttcaaat
     3361 ctagtgtgat aatttggtgc acctatatga atgtgtctgt caaatcttcc tggtctaagt
     3421 aaagcttcat ctaataagtc aagtctattg gtagcaccta ttattaatac attggaatct
     3481 ttattaaatc catccatttc aactaaaagt tggtttagtg tttggtcttt ttcgttgtta
     3541 ctttcaagat gtctttttgc tcccacagca tctatttcat ctataaatat tatacttgga
     3601 gcttcttttc tagctttttc aaatagagtt ctaactcttt ttgctcctac acctacatat
     3661 ttttcaacga actctgaacc agtaacattg aaaaatgatg agtttgtttc accggctaca
     3721 gcagatgcaa gtagagtttt acctgttcct ggaggaccat aaaataatac tcctttaggt
     3781 atttttgcac ccattttttg atatttttgt ggtgatttca taaaatcgac tatttcaaat
     3841 agttcttctt ttacttcgtc taatccagca acgtctctaa atgtagtctt tggttttgta
     3901 gaaagtgaat catctgtttg attgtctctt tccatttttg tttgtgcaaa gacaggagct
     3961 ggattgttaa ttttcttaaa aaagttgttt agcattttca tattcacacc tcaggtaatt
     4021 aattattgtt aatcaatttt atattttcca tatttactat tttttattat ttatttttca
     4081 tggaaaaaat atacatgtta tctaaataaa aataaacttg taagatgtat gaaaaagtga
     4141 atttatgtat atagagattt ctatttgtgt attgaataaa gtaatacaaa ttataaatta
     4201 tgtattgatt atatgttgag taaagtatta taaagcatgt attgattaag tattgaataa
     4261 aatattacaa atttagatga aatgaaaatg ataaaaaatg ttgtactata ctttacaaat
     4321 catataaatg gaaaaattgt tatagcacaa aatagtgagt agcatattag aatataatat
     4381 agaatttgtt gtgcagtata gtagtatatt agaatataat gtagaatttg ttgtgtagta
     4441 tagtagtata ttagaatata atatagaatt tgttgtgtag tatagtggta tattagaaga
     4501 caatatagga tttgttatgt agaatatatg gagtcattta gaatatacat attataaaag
     4561 gattttgggt ataaatttaa aaattaatag caaaatgagt agtatagaca tataattagt
     4621 agtaggaagt aattaaattt ttcctaaacc aaaaaaaaca gttgcaaata atttgattaa
     4681 gtgatatagt aaataaggta agaataaaac aaagaagaag tccgcttctc accttacgcc
     4741 agaggttagt aaggtaaata taagttaaag cttaatttca atttaagctt gtttaaattt
     4801 ggtaacttat attgcggatg attacgtcat ccgcttttta ttttataaaa attttggagg
     4861 tgtcctacaa ttagcaaaga attagcaatc aatgaacaaa taaaagataa ggaaataaga
     4921 gtcctttcac aaacaggtga acaacttgga gtaatgccta ctaaagaagc tcaagcaatg
     4981 gctaatagca aaaacttaga tttggttcaa atctcgccaa atgcaaatcc accagtatgt
     5041 aagataatgg attatggaaa gtttagatat gagcaagcga gaaaagagaa agaagctaag
     5101 aaaaaacaaa aaactatagt tgtaaaggaa gtaagactta gaccaggtat tgaacaaaat
     5161 gatttaaata cgaaagctaa taatgctata aagttcctta aaaaagggga taaagtcaaa
     5221 gttgaactta gatttagagg aagagaatta ggacataaag acataggtaa agaagttatg
     5281 cttaagtttt tagatataat aaaagagttt ggagaaccaa caaaagcccc tgcatttgag
     5341 ggtaataata tggttgtaat tatagatcca aaaaaataga tgattaactg agaggaggaa
     5401 tttgtcatgc ctaaaatgaa aactcataga ggtgcagcaa agagattaaa gaaaacagga
     5461 actggaaagt taaagagagc taaagcttat aaaaaacata tattaactaa aaaatctgca
     5521 aaaactaaaa tgaacttaag aaaatcaact ttagtaagtg atggagatgc taagagaata
     5581 gcacaattat taccatacta ggatacagat tataaataat aactatagaa gagggatata
     5641 aaaggaggtc ataacgatgg caagagttaa aaaagctatg aacgctcgta aaaagcataa
     5701 gaaaatatta aaacttgcaa aaggattcag aggatctaga agcaaattat atagaccagc
     5761 aaatacattc gtaatgaaag cattaaaaaa tgcttatata ggaagaaagt taaagaagag
     5821 agacttcaga aaactttgga tacaaagaat aaatgcagct gctagaatga atggaatatc
     5881 ttattcaaga ttaatgaatg gattaaaatt atctggtatt gaagttaaca gaaagatgtt
     5941 atctgaaatg gctatacaag acccagaagg atttgcaaaa ttagcagaag ttgctaaagc
     6001 aaaattagct tagtattgaa tcatgagatg taaagtcttt gagttatgta aataattcaa
     6061 gggctttttt attttttgaa aaatacttat tagtgccata tacattcaat tttataatga
     6121 taaataaaat aagagataca atagagtttg ctcagattta atataaaaat tattacaatt
     6181 atctataata actatagaaa gttattttaa aaataacatt gatatatgta taaagtttcc
     6241 agctaatctt gaattaatat tatcaatatg ataatattat cttgttaaga atatatgtag
     6301 agggagagat aatatgaatg ataaactgat ggattgtttt gtaaatccaa ttaaatgcaa
     6361 gttattactt gaaatttttt caaataatag aacgacagct aaacaattag gagaaaaatt
     6421 tcaagatata tcaaaggcaa cactttatcg ttaccttaat aaaatgttga aagatgatat
     6481 tataaaagtt gttgaagaaa ataaaataag gggaactgtt gaaaaagtat atgctatgaa
     6541 gttagatata gctgatgatg ttaacaaaat tatagatgag aactctggag aagaatatgc
     6601 tcaactgttc actcaatata tgatgggatt tttaagagaa ttttatgaat atgctgaaag
     6661 aaaggaaaag gatatcctaa aagatggttc agttttttct gtctgtccag tatatgctag
     6721 tattgacgag atagttgaag cgagtaaaaa aattgctagt atactagaac ctctgaaaaa
     6781 taattcacat gaagaaggta gaagaatgca ttctatagga gtgataatta ctccacctaa
     6841 agaagatgaa ggtaaataaa tgataaaagt aacagtttga tttttatgac aagctgttat
     6901 tttaatgtat agtattttat ttataatatt ttattttaat gtatgcaata aaatgtatat
     6961 taagctggca tataaagtga tatgatatat ctaagggaaa attgtatata tatgacatat
     7021 ttaaaacaca ggggatgaaa attatgaggt taattacacg ttctgatttt gacggattag
     7081 tatgtggtgc attattaaaa gaagcgggga ttattgatag ttggaagttt gctcatccta
     7141 aggatttaca agatggattg gttgaggtaa cagaagatga ttgccttgca aatgtaccat
     7201 ttgttgaggg gtgtggtctt tggtttgacc atcactctag tgaatttgag agaaataagc
     7261 ttgaaggaag atataaaggt gaaagtcgta ttgctccttc ttgtgcgaga attatttatg
     7321 aatattacgg tggaaatgaa agattttctc actttaatga aatgttagaa gcagtagaca
     7381 aagttgattc tggaaatctc actatagaag aggtacaaaa tccatctggc tggattttaa
     7441 ttggttatct tatggaccca agaacaggat tgggacgttg gagaaatttt actatatcta
     7501 actaccaatt aatggaaaag ttgattgatg catgtagaac tttaagtaca aaagaaattt
     7561 tagaacttcc agatgtaaag gagcgtatag aagtttattt tgagcaaaca aataaattta
     7621 aagagatgat tgctaaatat acacgtgtag aaggagatgt tattatttct gatttacgtg
     7681 gggtagagcc aatttacaca gggaatcgtt ttatgattta cagtatgtat ccagagcaaa
     7741 atatttctgc ttggattgta aatggtaagg gcggaaaagg gtgttctgta gctgttggat
     7801 acagtatttt aaatagaact tcgagtgttg atgttggtag cctgatgctt aaatatggtg
     7861 gcggtggaca caaagctgtt ggaacttgcc aatttaatga tgaacaaata gatgaattgc
     7921 ttccaaagct acttgatgag cttgtaaaca ctaaaaacta gtggataaac ttataaattt
     7981 taaaaactac tacgaataaa atagaacttc attgtaccat ttcatttaat gacttggagt
     8041 gatacaatga agttttttaa tttttcatat caataactaa agaaataaac tttagaccac
     8101 atatacaatt aggttacatt ataccattag aacagatata taaaataaaa aatcagtatt
     8161 tttatagcag acaaattgac tgttattctt tttaaaagtc atatattagt tgttgttaga
     8221 tggcgaaaaa aattatagga aactattgaa taaatgaata ttgcctataa tcatataaac
     8281 tgctatagtt taaaataaag cagaacgtta aattttatta taatcttaaa atttaagagg
     8341 gggattttat gaaaataaaa tttgttatta tgacattagt gatattttta atagcattac
     8401 tagtgggatg tcaaagtaca aaagatacag aagttaaagt aaaagataca gaaattaaaa
     8461 tattagggac aggtgattta cattcaattt tgacagactc tatggtttca tatgtaaatg
     8521 aggaaaggga aaagaataaa aatctgatta tggtagatgc aggagatttc tatggtacac
     8581 aaagtagaga aatgtgggag tggtcttctg gaaaaaagtt aattaatatt agaagagata
     8641 gtagggcaga gtatgatgac ataattaaga atagtaaaga tgaagtacct atagttaaag
     8701 agatggcaaa gttgaaatat gatgctgtag ttttgggaga taatgaattt gtttcaaatg
     8761 ataagcaaag tcttgataaa ctagtttctg attttaaaaa taataatatg ccattggtat
     8821 cagcaaatat atatgaacaa tctggagaaa attatgtaca accatatgta atgaaaaata
     8881 ttaagacaga tgagggcaat gtaaaagttg gaatactagg tcttactata aaagaagtag
     8941 gggaaaccct tggtcttgaa gattttgaaa aggataaaaa agcaagagcg ttaggagaac
     9001 aagctgatta caaaggaaaa ttatatgcaa atgatttaat agaggatgca aagaaatgga
     9061 taaaagttat gaaaaaagaa agtccagaca taatagttgc agttgtacac tcaggggaag
     9121 agcataaaat ggatagaaat ccaggaaata gaataaaaga gttagcaact acagtggatg
     9181 gaatagatgt aattgtagct ggtcatactc atgaaaaaat agatgaacat aaatataaaa
     9241 ataaatctgg agatgaagta attgtcacac aaccaggttg gcatggagat agagtgtcag
     9301 agattaattt caaattaaat aaagaaaatg acaagtgggt tataaaggaa aagtctagta
     9361 aattaaaaat gattgattaa aaaatagcta cgcacacttg tgactttagt
//
LOCUS       DAMJVM010000121         9388 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.126, whole genome shotgun sequence.
ACCESSION   DAMJVM010000121 DAMJVM010000000
VERSION     DAMJVM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9388
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.126"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            358..1563
                     /locus_tag="PC523_003134"
     CDS             358..1563
                     /locus_tag="PC523_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2476629.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTDHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKD
                     TVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLISRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1634..1855)
                     /locus_tag="PC523_003135"
     CDS             complement(1634..1855)
                     /locus_tag="PC523_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476630.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(1997..2596)
                     /gene="recR"
                     /locus_tag="PC523_003136"
     CDS             complement(1997..2596)
                     /gene="recR"
                     /locus_tag="PC523_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDF2476631.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2609..2959)
                     /locus_tag="PC523_003137"
     CDS             complement(2609..2959)
                     /locus_tag="PC523_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDF2476632.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(3018..4655)
                     /gene="dnaX"
                     /locus_tag="PC523_003138"
     CDS             complement(3018..4655)
                     /gene="dnaX"
                     /locus_tag="PC523_003138"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDF2476633.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(4732..4996)
                     /gene="ffs"
                     /locus_tag="PC523_003139"
     ncRNA           complement(4732..4996)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PC523_003139"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(5034..5125)
                     /locus_tag="PC523_003140"
     tRNA            complement(5034..5125)
                     /locus_tag="PC523_003140"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5089..5091),aa:Ser,seq:gga)
     gene            complement(5126..5581)
                     /locus_tag="PC523_003141"
     CDS             complement(5126..5581)
                     /locus_tag="PC523_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HDF2476634.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            complement(5903..7174)
                     /gene="serS"
                     /locus_tag="PC523_003142"
     CDS             complement(5903..7174)
                     /gene="serS"
                     /locus_tag="PC523_003142"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDF2476635.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEKEFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKNIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYALM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIR"
     misc_binding    complement(7299..7505)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7633..8514)
                     /locus_tag="PC523_003143"
     CDS             complement(7633..8514)
                     /locus_tag="PC523_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDF2476636.1"
                     /translation="MNFLSLAKSASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDLVKSILNEIFINEKR
                     ILDDPRPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENNIKVPFVP
                     SSLLFNNMSNLNTMDKR"
     gene            complement(8526..9035)
                     /locus_tag="PC523_003144"
     CDS             complement(8526..9035)
                     /locus_tag="PC523_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDF2476637.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIAII
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
     gene            complement(9163..9279)
                     /gene="rrf"
                     /locus_tag="PC523_003145"
     rRNA            complement(9163..9279)
                     /gene="rrf"
                     /locus_tag="PC523_003145"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         3010 a         1652 c         1003 g         3723 t
ORIGIN      
        1 catctttatc ctgtctagtc aatataattt tttaattttt ttatttatca tatattcttt
       61 atatttaatt tccaaaacct aatgctaaac aaatctgttt atttcataaa actaatatat
      121 attctatgct ctattttcct ttttattcta tatttaaatt ttttatttac aataatattt
      181 ttaacataac atatttgata aaattaaatt tcatttcttt cacttgtaat ttttgtaatc
      241 ttgtattaga ttaatattaa aaaatagttt atcaataacc ttatttacaa agacagtttt
      301 atatatcaaa acataataca atataaatac tatgctacat tttaggagga ttttattatg
      361 agcaaaaaag tattaataat gtctgcttcc actggtggtg gacacaatag agctgctcta
      421 gctattaaag aagaacttac ttcaaaaaca cttgatggag agccaattga atgcgaaata
      481 atagatagtt tgaaattagt taataataca atggacaaga taatttcaag aggatacgaa
      541 aaatctgcta tatatactcc caaagcctat ggaagtgtat atagattatc tgaaacaaat
      601 cttttatcaa aaaatgaatt taaagataat ctactaataa cattcatggc taaaaaattt
      661 aaaaagctta ttagaagcga aaaaccagat ttgattatag gaacgcatcc ttttccaatg
      721 attgctctta gtacattgaa aaaaaacttt aatttacata ataatgaatc aaatgcttat
      781 acagatcatt tttataaaca ttatacaaat accattaatg ttcctccgct tatttctgta
      841 ttaactgatt atactactca ctctacatgg atacaaaatg aaattgatta ctatattgtt
      901 ggtcatgaat atgtaaaaga actattggtg tttgatggag ttgaaccgag taaaattaga
      961 acatttggaa tccctgttga aaaatctttt ctctctcaca gagataaaga tactgttctt
     1021 tctgagttga atctatctcc tgataaatta actgttttac ttatgggggg gagctttggg
     1081 gctggaaata taaaagaaac tcttgatgaa ttattggata ctgatagaga ctttcaaata
     1141 cttgtaatta ctggtaagaa tgaaagccta aaagaaaaaa tcgaaaaaaa acttatatct
     1201 cgttatcatg ataaaaatgt atgtgttctt ggatatactg ataagatgaa tgatatttta
     1261 gcttctatag atgtattagt aagcaaacct ggtggactta ctacaactga ggctctttta
     1321 aaagatgtac ctatgatagt tccttattat ataccaggtc aggaagaaga aaatcttgat
     1381 ttcttatcta attgtggtgc agcgcttaga actacaaaaa aatataactt gtcaatatta
     1441 ctaaaagttt taatagatga ccctagcaga ttagaaatgc taaaaagaaa tattaaatca
     1501 ataagaaagt caaattcagc ccaaaatata gctaatttaa ttttaaatat aatttctggt
     1561 tgataaaata aataaaatcc ctatagtata tatattatct attagggatt ttttattttc
     1621 taattattta atattaaatt ggttcataaa agtcttcttc gtaatcatac tgataatttt
     1681 tataattatt tctaaattca gcatttttat ttcgtacatt ttttttagtt tttttcttat
     1741 tagaaaaact tatatcctca aaatcatcat atttaggttt atcttttgat ttcatttttt
     1801 taactctttt ttctttcatt gcattatgtt cttctaagtt cataattggt ttcatgttgc
     1861 acccctatca aaatttttaa atgatttata caacaaatta tacaatacag aagaaattta
     1921 ttcaataaat ctaaatcaaa attaatttga atttaaccca aaaaaattac aaaaatcgag
     1981 gtattaacct cgatttttat atttctcttc tgccttctag tgcttttgat attgtgacct
     2041 catctgcata ctctaaatcg cctccaacag gaagaccatg agctatccga gtgactttga
     2101 ttcccattgg ttttactaac ctagcaatat acatggctgt agcttcacct tcaatagtag
     2161 gatttgttgc cattattatt tctttgactt cctgattgcc aagtctttgg ataagttcct
     2221 ttatttttaa catatctggt ccaattccat ccattggaga aattactcca tttaatacat
     2281 gatactggcc tttaaactct ctcgttctct ccatagctgc tacatctctt ggatcttcca
     2341 caacacatat aacacttgaa tctctacttt tatttgaaca catgctacat gggtctgtat
     2401 ctgttatatt acaacaaatt gagcaatatc ttatttctct ttttgcatct ataatcgctt
     2461 tagataaagc ttccacatca ttagtattca tgttaataat atgaaaagcc aatctttgag
     2521 cagtctttct tcctactcca ggaagctttg aaaattcctc tataagtctc gttattggac
     2581 ctgtataaac ttgcataaat accacctact agaataaccc aggtatattc atacctccag
     2641 ttaccttact catttgactt gcttgtatat catcaatatt tctcaatgct tgatttactg
     2701 cacttaaaac taaatcttgt agcatttcta tatcatctgg atcaactact tctggtttga
     2761 tagttatatc tataacttct tttttaccat taacttttac agtaactgct cctccaccaa
     2821 ctgaagcttc tacttcttta ctttctaact cttgttgagc tttttgcatg ttttcttgca
     2881 ttttttgagc ctgctttaac aagttgttca tatttccacc accaggcatc attccgcctc
     2941 caaaaccttt tttagccata aacactattc ctcctaaact tattttcatt ttattattat
     3001 atcataatag tattttatta tttactctca ttttcatcaa tactatcttt tatttctaat
     3061 atctcttcac tcactatgtt ctttaatatc tcttcaccaa tgtccttttt ctctttaact
     3121 tcaagattta tatctttaac ttctgatgtc agtactattt ttacattaaa acttctattt
     3181 aaaacatctc ttattgtttt ttctaaataa tttattgtat cttgagagct cagtctagtt
     3241 tttgcaaaat caaatttatc atcaaatata acatacaaca tattagaata tacattaaaa
     3301 ctttttactt cacttaagag tgcgtatatt ggcatttttt tatctttttt aatttgttta
     3361 agtatattct tccaagatga ctctataagt ataacgtcct cattttttac atcttcataa
     3421 actacatttt cttgttttac atctatttct ctatcaacct catactcttt ttctttatta
     3481 ttcccaattt tttcagactt aaagttcccc agttcaatca ttttttctag gttctcaact
     3541 ctttttataa gagcttcttt actttcatca aacattggtt gagcaatttt cattatggtt
     3601 atttccacta aaacccttgg atttgaagat gactttatat catcctgagt tattgataat
     3661 atatttaaaa tccttattaa atcatttata tctatatttt tagattgaat tttaagttgt
     3721 tctatagttt cttctggtaa tgaaattatt tcatccaatt ctgatgaaac cttacatacc
     3781 attaaatttc taaaatgatc aattaaatca tttataagat ttcttatatc tttgccccat
     3841 aagacaaatt catttaatat ctcaagagac tttttagtgt cctgctctac aatacattga
     3901 gacaattcaa ataattgttc tatatttacg cttcccatta attcaacaac atctttataa
     3961 tctatcttac tttctccaaa agacatacat tggtctaata tactaagtgc atctctaaga
     4021 gctccttgag aatttctagc tattaaatta agagctttat catctatatc aattccttca
     4081 tcttcacaaa tctttttcat tctaagggtc atatctttaa ctgtaactct tttaaaatcg
     4141 tatctttggc atcttgataa aatggttgca ggtattttgt gaggctctgt agttgctaat
     4201 ataaaaatta catatgatgg aggttcctct aaggtcttta ataatgcatt aaaagctcct
     4261 tgagacaaca tatgaacttc atctattata tatactttat atttagcatt ggccggagaa
     4321 tatttcacac tttctctaag ctctcttata tcatctacac tgttatttga ggctgcatcg
     4381 atttctacaa catccattgt attatctttt aatacagatt cgcatatctc acactcatta
     4441 cacggttctt catttatttt attcaaacag tttactgctc tcgaaaaaat ctttgcagta
     4501 gatgttttac cagtacctct tgttccacaa aacaaataag catgtcctat attatcacta
     4561 tatatttggt tttttaaagt cttaataata tgatcttgac caataacatc ttcaaacttt
     4621 tgtggtctat atgctctata taatgcttta tgcatctttt tacctctctt tagcaaaatt
     4681 aataaaaaaa tccctcatta tctaagggat tagtctttta tatattaaat gctgtacacc
     4741 atctatcgac ttaattccat aggcgttacc aaaacagtta gctcaactta ggcaccccca
     4801 cggcacacaa aagtgatcac ttaccgctgc ttccttccgg atctgacggg gttcatgagc
     4861 tttcattgcg caggacctaa gcatcaacac cacttattta gggcggccct acagaaaata
     4921 agcctttaga tggaattcaa tcctgctata gcggattgca ggttacaggg caccgctacc
     4981 tccccatcta gtacagcaaa attacaacca tatttaatta ctttcattgt ttctggcgga
     5041 gagagtggga tttgaaccca cgatacagtt ttacccatat actcgctttc caggcgagcg
     5101 ccttcgacca ctcgaccatc tctccttaat ctctttttct caatgcctta aaaaattctt
     5161 gcaatatgat cgagcattct tcttgcaaaa catcaaaagc tacttttaca ttattattat
     5221 taaaataatc taatttaaac tcatgctgtt tctctatata tgaatttttt acatgtcttg
     5281 tccctattat aagctttttt attctagaat taactattgc accactacac attatgcaag
     5341 gttccattgt aacatataaa tcacaatcag tcaacctcca accacctaat ttttctgatg
     5401 cttgttttat cgctaaaatc tctgcatgag ctgtagagtc ttttaacgtt tctgtaaggt
     5461 tatgagctct agctattatt tgattatctt taactataat agcacctatt ggtgtttctt
     5521 ttttattata tgctttatat gcttctttta atgcttcttt catataaaat gaactttcca
     5581 taattcctcc caaaacttat aacaaactta tcttatcata attcatttta taattcaact
     5641 taattttatt ttgtttattt aattatcact atatatagct atattttttt aatattagtt
     5701 ctaaaataat ttttagatat ttatacaaga gtattttata aaaatattta gtgttttatg
     5761 atgaaaattc tgtcatataa attaacataa aaaaactatg aaatttaata tttacaacaa
     5821 tttcatagtc ttttaattta ttgtttactc ataacatctt ttcttcatta aagcatattc
     5881 tatacataca attaatataa aattatctta taacatcaac acccatataa ggtcttaatt
     5941 cttctggaac tactacagaa ccatctgcct gctgataatt ttctaatata gcagccaaac
     6001 atcttcctat cgctaatcca gaaccattca atgtatgaac atattctgct tttgattttt
     6061 tatctctttt aaatcttatt cctgctcttc ttgcttggaa atcttcaaaa tttgaacaag
     6121 aagatatttc tacatatcta ttgtaacttg gcatccaaac ttctaaatcg tatttaaatg
     6181 cagctgtaaa tcccaaatcc cctgtacata tttttacaac tctatatgga agacctaaca
     6241 tttgcaacat agtttcagca tcatgagtta atgattctaa ttcattataa gaatcttctg
     6301 gttttacaaa tttaactaat tcaactttat taaattgatg ttgtctaact aaacctcttg
     6361 tatctcttcc tgctgaacct gcttcagatc taaaacaagg agtatatgca caatatttaa
     6421 ttggaagttc tgcaacatct aatatctcat tagcatgtat attagtaaca ggtacttcag
     6481 ctgttggtat taagaaatac tctaatcctt ctatcttgaa catatcttct tcaaatttag
     6541 gaagttgacc tgtacctata aaactatttc tattagccat aaatggaggt aaaacttctg
     6601 tatatccatg tttagctgta tgagtattta aaaagaaatt cattaaagat ctttctaatc
     6661 tagcgcctaa acctctatat aaagtaaatc ttgaaccagt tatttttcca gctctttcaa
     6721 aatccaatat acctagacca gtaccaatat cccaatgtgc tttgtgttcg aaatcaaatt
     6781 tagtaggctc tccccaagtt cttatttgaa cattatcttc atctgtttca ccttgtggta
     6841 cctctggatg aggaacattt ggtattctca tcaatgcgta ttccattttt gcttctactt
     6901 cttttacttt ctcatcaata ccttttattt tatctgataa ctcttttaac cttgcctttt
     6961 cttctgttac atcctttcct tcttttatta attgtggtat atttttagaa tttacattta
     7021 attcattttt cataacctca acttcttgaa gtatctctct tcttttatta tctaattcaa
     7081 ccacagcatc aaggtcaaat tctttttcgc ctcttctttc catagctttc ttgatatcat
     7141 ctaaattttc tcttattctt ttaatatcta acatattaat tcctcctaat aattttgata
     7201 atataaatta taattccaat taatttacaa ttattctagt tgtaaacctt taatttaaac
     7261 aaatttgaaa aataaaaaaa gacctccgtt taccctataa aagggacgaa aatcttttat
     7321 ccgcgttgcc accctaattg attatactaa aatgtataat cctctcagac ctataacgta
     7381 ggtaaaccgt ctagctttaa ctagaagctc tagagtagat tcaaaaacca ttgtaatgag
     7441 ttctcaccat ccactcattc tctgaataca atctgttttt tactattttc cttcacagct
     7501 ttttaactaa ttgttttatt tgataatcat tatattatat atattaacat ctttcaatac
     7561 tattattcaa atttttttag aattttcttt aattttttat attaaattca ctttttttac
     7621 ttaattaaaa ttctatcttt tatccattgt atttaaattg ctcatattat taaataataa
     7681 tgaggaagga acaaatggaa cttttatatt attctcatca aatttatttt tgactttttc
     7741 cattacatca aaataaacat cccagtaatc atcagtttta acccatggtc tagcaactat
     7801 ttgcatggta ttatttccta tagagtttaa acctataatt ggtcttgggt catccaatat
     7861 ccttttttca tttataaaaa tttcattcaa tatagactta actaaatcga tatcactatc
     7921 atatgaaata ttatatgaaa aatctatcct tcttttattt tgatgtgtgt aattaattat
     7981 attgttactt gtcaattgaa agtttggtat aattatggtt ttattatctg gagtcttaag
     8041 aacagtacta aatatttgta tgtcagaaac tgtaccttct acacctgaac cttgaatata
     8101 atcacctata ttaaatggct taaaaaacaa aataatcatg cccgaaccta aatttgataa
     8161 tatttcttta aatgctaaac ctatactaaa tccagcagct cctaacacag ctacaactga
     8221 tgtagtttgt attcctataa tccctaaact tatcaatgat ataatagata acatagatat
     8281 atatattcca tatacaatga aattatttac acctttactc attccatatt tgattagaaa
     8341 ctttgaaacc atccttctta ctattttaga tatataaata cctatcacaa atacaattat
     8401 tgcatatata atatttggta tattatcact aaaattatcg agcatatttt ctaaagttct
     8461 cttagttatg ttactatttg ttgaggcaga cttagctaat gataaaaaat tcatttaact
     8521 cctccttacg ataagattcc gttgtccttt aactctttta ctatagcttc tatagtttcc
     8581 tcttttattg cctcaattgt atgaatatgg ctacattctg tcagaaccga taattgttta
     8641 aattcattag tctcaacttt cttcataaag ttgtctatat ccattttaga ataaatatta
     8701 agttctgctg ttatctgacc atatgttgga tgatttacga tgacatcttt tataatacca
     8761 cctaaatcta caattatctt taattcatca taaatttctt tagaatcttt atggttttta
     8821 cattttattt gttttgtaaa atacggttga ttatatatta tatatccttg aggtgtagct
     8881 attatttcaa atcctctagc tcttataatg gctatatctt gtactattac ttgtctactt
     8941 acatttaata gttcagctag tgttccacct tttacaggat gcttagattc tttcaaaata
     9001 tctattaatt tacttcttct ttcattagaa ttcatattac accttctttc caaatcaaaa
     9061 actttaccta tattatataa ttctatattt tatcttatta taaatcaata ttgactttat
     9121 attctattta atcttaattt ttataataaa tgaaaaaaga ctacgtggct acgtcctact
     9181 ctcccaggca gtttcccacc aagtaccatc agcgctagag agcttaactt ctgtgttcgg
     9241 aatgggaaca ggtgtatcct ctctgctatt gtaaccacat aatctttatg cttaatattt
     9301 ctattaagtt tttaataatt ttttagaatc tagtaatact agttctttta gagttaacac
     9361 tctaaaaact acacatatat cttagtaa
//
LOCUS       DAMJVM010000122         9065 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.127, whole genome shotgun sequence.
ACCESSION   DAMJVM010000122 DAMJVM010000000
VERSION     DAMJVM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9065
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.127"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            234..1610
                     /locus_tag="PC523_003146"
     CDS             234..1610
                     /locus_tag="PC523_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDF2476638.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSRGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVGKRRFSKLNVHSKVVLFSTATLILVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPATIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            1910..2581
                     /locus_tag="PC523_003147"
     CDS             1910..2581
                     /locus_tag="PC523_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDF2476639.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            2768..3565
                     /locus_tag="PC523_003148"
     CDS             2768..3565
                     /locus_tag="PC523_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDF2476640.1"
                     /translation="MITNINSKDNEKLKYTKALLKSKNRNKESKFIIEGYRIVVLALE
                     CMANLDYVFINEDFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVEAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIINASQDETVDMLKSLDFNIVSSYLNTDNFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKSLI"
     misc_binding    3826..4060
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4110..5129
                     /gene="pheS"
                     /locus_tag="PC523_003149"
     CDS             4110..5129
                     /gene="pheS"
                     /locus_tag="PC523_003149"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDF2476641.1"
                     /translation="MQEKLLALREVALAEIKEAQSIESVESLRVKYLGKKGDITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     gene            5146..7539
                     /locus_tag="PC523_003150"
     CDS             5146..7539
                     /locus_tag="PC523_003150"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDF2476642.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     NDALIDFELTSNRPDCKCMLGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRTAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITDD
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVADDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNVKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFVLKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEEVAKVHDKILSELSEKLNANLRSN"
     gene            7564..8154
                     /gene="zapA"
                     /locus_tag="PC523_003151"
     CDS             7564..8154
                     /gene="zapA"
                     /locus_tag="PC523_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDF2476643.1"
                     /translation="MNKVMVKIHGVEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAEAKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDVKNVE"
     gene            8353..9036
                     /locus_tag="PC523_003152"
     CDS             8353..9036
                     /locus_tag="PC523_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDF2476644.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKAKIHWRQVIIIIEMI
                     TILIVSFVPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFIFGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
BASE COUNT         3494 a          990 c         1748 g         2833 t
ORIGIN      
        1 gatagtttta gacctattat atttaaaaaa tatagatgtt tttggtgcaa tttaaattaa
       61 aagatatata caaaaattta aaatattcca aacagaaatt ataaaaatta ctatcattgt
      121 ctttatacaa tatttatact ataatagaat aataatttag aggaaagctc taaaaataaa
      181 caaattaagg gtcttaacag tagtgatttt atgttaagga ggtataaaga aagttgaaaa
      241 caatagttag aagacttatt ctctacgcag atggaatgcg tcctactcaa ataatggtat
      301 ctgggtttgc tgcgattatt gtaatcggag cattgttgtt aacactcccg atagcgtcgc
      361 agagtggaga gagtataggc ttattaaatg ctttatttac ggctacttca gcggtttgtg
      421 ttactggtct agttatggta gatacagcaa cttattggag tttatttgga cagatagtta
      481 taataacatt gatacaaatt ggtggtcttg gatttatgac cgtagccaca atgttttcac
      541 ttatggcacg aaaaaaaata caactaagag aaagattatt aatacaggag tcattaaatc
      601 aagctgattt atcggggctt gtaagactta caagatttgt attaataata accataacaa
      661 tagagggaat aggtgcacta gttttatcaa ctgtctttat tccacaattt ggtttgtcta
      721 gagggatatg gtatagtgta tttcacgcaa tatcagcgtt ttgtaatgcc ggatttgact
      781 tgatgggcag tgtaagtggt ccatttacat cgttaaattc ttatgtaaac aactttacag
      841 tatcaatgac tgtttgtgca cttatagtgc ttggagggct aggattccct gttgtacttg
      901 atattgtagg aaaaagaaga ttttcaaaat taaatgtgca ttctaaggtt gtattgtttt
      961 caactgcgac acttatttta gtgggtgcat tatttatttt tcttatagaa tttaatcaaa
     1021 aagctactat ggcggacttg cctttaaagg gaaaagtact atcagcaata tttcaatcag
     1081 taacagcaag aactgctggt tttaatacgc ttgatttagc cactttacga gaaagtagtg
     1141 tatttgtaat gataattttg atgtttattg gagcatcacc tgcatcaact ggtggaggta
     1201 taaaaacaac tacactagct gttttaatca ttacagttag aagttttcta tcaggaaagt
     1261 cagatataga agcttttgaa agaagattag caccagcaac tataaaaaaa tctctgggta
     1321 tatttgtaat tagtatatca gcagttattt ttggaacatt gattatttca ataactcaac
     1381 ctaattttac attagttcaa tcggcttttg aggttacatc agcacttgct acagttggtt
     1441 caagtttagc tggaactcca aacttaaatg ctttaggtaa gattataata ataatcttta
     1501 tgtttatggg tagagttggt tctcttactt tatttatggc tatactttct ggtggtagaa
     1561 gaaagagcca accaatcaga tatgcagaag gtaaaattat ggttggttaa tttaccaagg
     1621 agtagtataa atttataatg ggtttagagt agtaatgtaa atctgttgat aaaataaatg
     1681 agcctagatg taattatatg aagaaataaa atcgactaat gagcaaaatt aattaatgaa
     1741 ataaaaaata gtgaaataaa aaatagtgaa ataaaataca aataaaagat aaatcaaaat
     1801 taaattaatc tatgtatata aaattataga ttgtatacaa gttttgtatg cataaccgat
     1861 taatttaata gatattatat aaattgtgta ttagggagga agtttcgaga tgaagcaata
     1921 tatagtcatt gggtgtggga gatttggaag ttcagttgcg tctactatgc atcttttagg
     1981 acatcaagta atggcaatag acaaaaatga agattcagtt caaagtatat ctgacaaggt
     2041 aacccattca cttatagtgg atgttactga tgagcaagcg ttaaggtcat taggtttagg
     2101 taactttgat gtagcagtag ttgcaatagg ttctgatata agggcatcta taatggcgac
     2161 tcttatagcc aaagaaatgg gtgtagagtt gataatatgt aaggcaaagg atgaattaca
     2221 agctaaagta ctttataaaa ttggcgcaga tagagttgta tttccagaaa gagatatggg
     2281 agtaagagtt gcacacaatt tagtttcaga taatatatta gaccatattg aacttgaccc
     2341 agagtattca attgttgaaa tcgtaactcc aaatagttgg gttggcaaga cacttataga
     2401 gcttgaatta agagctagat atgagataac tgtacttgct ataaaaacag gtaaaaatat
     2461 aaatgttaca ccttctccag atgaggaact tacagccgga agtattctag ttataattgg
     2521 tcaaaatact agtataacag ctataacatc tggaaataag gggataatta gaagaagata
     2581 atttattatt taatatatat ttaattgcaa tgaaagtaga tagtatcata taattatgaa
     2641 tagttatatg atactatttt tatttaatca aaagtaggat ttagatgtaa gattaggtaa
     2701 agagtatttt aagttaaaaa aggagtaagg aggttgtgct aatatagaaa tttagcattt
     2761 agataaaatg attacgaata taaacagtaa ggataatgaa aaattaaagt atacgaaagc
     2821 actgttaaaa tcaaaaaaca ggaataaaga atcaaagttc ataatagaag gttacagaat
     2881 agtagtactt gcacttgaat gtatggcaaa tcttgattat gtatttatca atgaagattt
     2941 tgaaaataag aaagaacatg taaaactatt agaagattta gataaaaaaa acacaaagat
     3001 atataagact actaataaaa actttaaaga attagtggat acagaaaata ctcaaggtat
     3061 aataggtgta gtttcattta agaaaaaaaa attaagtgaa agtataaata aaaaagataa
     3121 atttgtattg attttagaca gaatacaaga cccaggaaat atggggacta taataaggac
     3181 tgctgattct gctggggtag aggccataat agcactaaag ggatgtgtcg atatatacaa
     3241 cccaaaagta attaggtcta ctatgggttc tatttttgat atgaatataa ttaatgcttc
     3301 acaagatgaa actgtggaca tgcttaaatc attggatttt aatatagttt caagttactt
     3361 aaatacagat aatttttatg acaaaataga ttatggttca aaggtagcat tggtgatagg
     3421 aaatgaagca aatggaataa atgaagaact tgtatcaaag tctgatattt tggttaagat
     3481 acctatatat ggtaaagctg agtcattaaa tgctgcgata agctctgcta tactgatgta
     3541 tgaaataaaa aaatccttaa tttaatgtat tgtaataaat ataatgtcat gttataatct
     3601 tgaattaaat agtagagtat caaaaatagt caatatatat taaaaaaata gttcaattaa
     3661 ttatatatta gatgtgtatc atataaaaaa tgattgaatc aattatatat tagatgtata
     3721 ttatataaag aaataattga attaattata tattagaggt atattaaaaa tagtcacata
     3781 atttgaatgg aaatgatata atactaaaat aaacaatatc atattgtaaa tgcaatgaaa
     3841 gaggaaagta ttttgattaa acttagtaaa gagataaaca cctaggctgg gagtgttttc
     3901 taagaggtca cgagaagttc cctctggagt aacagagctg aaattttata gtaggctttg
     3961 acgtcaaaaa cgcgttaagt ttgttagagg tggtttgatg atttttaaat tgttaaacta
     4021 ctagggtggt accgcgaaac tatagccttt cgtccctaga cagggattga agggcttttt
     4081 ttatacaaaa aaacgaaagg gtgatatgtg tgcaagaaaa attacttgct ttacgtgaag
     4141 tagctttggc tgaaataaaa gaggcacaaa gcatagaaag tgtagaaagt ttaagagtta
     4201 agtacttagg aaaaaaaggt gatataactg ccatacttaa agaaatgggt aaattatcag
     4261 ccgaggaaag accagtagtt ggtaaggttg ccaatgaggt aagagaaaat attgaactta
     4321 gcataaattc taaaaaagaa gaaataaatg ctattgaaaa agaaagaaaa ttaaaagaag
     4381 aagtaataga tgttactcaa ccaggaaaag ttttaaaggt tggaaagaaa catccaataa
     4441 ctcaaattat agatgaagta acagatatat ttatcggaat gggattctct atagcagaag
     4501 gtccagaagt tgagactgtt gaaaacaact ttgatgcttt aaatgctcct aaagaccatc
     4561 catcaagaga tatgagcgac acattctata tcaatgatgg ggtattactt agaactcaaa
     4621 catctccagt tcaagtaaga actatgagaa gtcaagaatt accaataaaa gtaattgcac
     4681 caggtagatg ttttaggtca gattcaccag atgccacaca ctcaccaatg ttccatcaga
     4741 tagaagggct tgttgttgga aaagatgtta ctatggcaga atttaaagga actatggata
     4801 tcttcgttga aaaattgttt ggttctgata tcaaaactaa gtttagacct cataacttcc
     4861 catttacaga gccaagtgca gaggttgacg ttacttgttt caaatgtggt ggtaaaggtt
     4921 gcccaatgtg taaatatgaa ggttggatag aaatattagg ttcaggtatg gttcatccaa
     4981 acgtgcttag aaattgtgga atagacccag aagtttatag cggatttgca tttggagttg
     5041 gggttgaaag acttgcaatg cttaaatacg aaatagatga tattagatta ttattcgaaa
     5101 atgatatgag attcttaaat caattttaat taggagggta tgtagatgtt agtatcttta
     5161 aaatggctta gagactatgt tgatatagac atggatgtaa aagagttcgc tgataaaatg
     5221 acaatgacag gaactaaagt tgagacaata gattattatg gtgaagaaat agaaaatata
     5281 ttggttggaa agattttaga aataaagcaa catccaaatg ctgataagtt ggttgtaact
     5341 aaagtagata ttggagataa agttgttcaa atagttacag gagctacaaa tatatcagaa
     5401 ggagattata ttccagtagc tgtaaatggt tctaaattac ctggaggagt ggaaatcaaa
     5461 cagactgatt ttagaggtga attatcagat ggtatgatgt gttcagcagc tgaactaggt
     5521 atagatgaac attacattga agagtataaa agaggtggta tatatatttt agaccacgaa
     5581 gattcttatg aattaggaaa agatataaaa gatgttttag gattaaatga cgctttaata
     5641 gattttgaat taacttcaaa cagacctgat tgtaaatgta tgctgggtat agctagagaa
     5701 gcagctgcaa ctataggaac aaaagtaaaa tatcctgaaa ttgaagtaaa agaaagtgat
     5761 gaagagatag acttcaaagt tgagatagac aatccagatt tatgtagaag atatgttgct
     5821 agaatggtta cagatgtaaa aatagaacct tctccatatt ggatgcaaag aagacttaca
     5881 gaagcaggtg taagacctat aagtaacata gtcgatataa caaacttcgt aatgttagag
     5941 cttggtcaac cacttcatgc ttttgatata aatcaagtag aaactggaag aatagtagta
     6001 agaactgcta aagatggaga aaaacttgta acattagatg atgttgagag aacattagac
     6061 aaagatatgt tagttataac aaatggagaa aaatcacttg gtttagctgg tgtaatgggt
     6121 ggtgctaact cagaaataac tgatgataca aagactgtac tttttgaaag tgccaatttc
     6181 aaaccagaaa acataagaat gacagctaaa aaagttggta ttaggtcaga agcatcttca
     6241 agaaatgaaa aagacttaga ccctaatctt gcagagatag cagcaaatag agctgcacaa
     6301 cttgttgaaa tgttaggagc aggaaaagtt ttaaaaggtg ttgtagatgt atatccaaat
     6361 aaaccagaac ctaaaaaatt ggtagtaaat cctcaaagaa ttaaccattt attaggtgta
     6421 gatgtaccaa tggagcagtt tgtaggaatc ttagaatcat tagaatttaa atgtaattta
     6481 gtagctgatg ataaattaga aatagatgta ccaagcttta gaacagatat ggaacaagaa
     6541 gctgatgtat gggaagaaat agctagaatt tatggatttg agaatatacc atctgtacaa
     6601 ttagaaggaa atacgacagc aggaatcaaa acttcaaaac aaaagtttat ggatgcttta
     6661 aaagataatt caactgctgt aggactaaat gaaatattaa catattcatt tgtaagccct
     6721 aaaggagtag ataagataag agtaccagaa ggtaatgtga agagaaattt tgttaagcta
     6781 cttaatccat taggagaaga aacttctgta atgagaacta ctttaatacc aaatatgtta
     6841 gatgttttat caactaatgt atctcataaa atagaagaag tatctgcatt tgagtgtgga
     6901 catatattca tcccacaaga ttcagaatta cctaaggaag aaaatagaat gtgtgtaggt
     6961 atgtacggta aagatgttga tttctttgta ctaaaaggaa ctatagaaac tatacttgta
     7021 aatgtaggat ttaaaggtta tgaaattgaa cctcaagata ataatactac cttccaccct
     7081 ggtagatgtg ctaaaatagt gtataataat aaatatgtag gtacattagg tgaattgcat
     7141 ccagatgtta tagaaaacta caatctaggt caaagagttt atgtcgctga aatagatatt
     7201 gactttgtat ttgataattc tgatagaaca aagaattatg taccattacc aaaatatcca
     7261 tctacatcaa gagatatagc tcttattgtt aaagatgatg tatttgtaaa acagatagaa
     7321 gatataataa aggaaaatgg tcaaggatta gtagaaagct acaagttatt tgatgtttac
     7381 aaaggttctc aaatagaagc tggatacaag tcaattgcat attcaataac ttatagaagt
     7441 aaggataaaa ctctgactga tgaagaggta gctaaggtgc atgataagat attaagtgaa
     7501 ttaagcgaaa aattgaatgc taacttaaga tcaaattaac aactaatgtc gggggaggtt
     7561 aggatgaaca aagtaatggt taaaatccat ggcgttgaat atccaatggt tggagacaaa
     7621 tcagagaagt ttatgattag tattgctgat tttgtagaca aagaaatgga taaaataaca
     7681 cgtcaaaacc cgaaattgag cttatcagtt gctgcgatat tgactgcatt aaatatatca
     7741 gaccttttat ttgaatgttc agatgagaat gagaagttga ttaaagctaa tgaagaattg
     7801 agtaagaagg ttggagcatc taatgaagag cttcaattag aaatcaagtc tttaaagctt
     7861 acgatagcag aaaaagaagc agaaaatcgt gaagctgagg ctaagatgaa agagttaatt
     7921 gagattatag aaaataaaaa gcaagaaata ttcgaactta gtaatacgac agaaggttct
     7981 agggcggagt tagatgctta taaaaacaag attgaggagt taagtactca acttgaagaa
     8041 gcaaatgaaa gagctaccat agctgaaaat ttagcttctg agtttcaaaa caaagcttat
     8101 gatttacagc taaaatgtac agggttaaat aatgatgtaa aaaacgtgga ataaattaaa
     8161 agatttgctg tctcttgttt tttgagacag cttcttttaa tgtataaata taaatgataa
     8221 atataaataa atataaatga tgtataaata tagacaatat ttaaatataa atgatttttt
     8281 tatattaatg aaaaatagaa tctaaaatga ttttgatttt gtatctatca ttagaaagtg
     8341 agaggtttat atatgacaag caagagtcag atgtccgaat cattcttttt atgttcttta
     8401 ttggcaataa caggaggatt tttagatatt tatacttatg tggtaagagg taaagtattt
     8461 gctaatgcac aaactggaaa tatagttcta tttgggctta atatagcaga aggtaatgta
     8521 aaggaatcat tttattattt gattcctatt ttggcattta tgttaggcgt atttatagct
     8581 gagaagataa gaaagtattt tacaaacaat aaggcaaaga ttcattggcg tcaggtcatc
     8641 attataattg aaatgattac aatacttatt gtttcatttg ttcctagagg aagatttaat
     8701 atgtttgtaa atgttgccat ttcttttatt tgttcaatgc aagtggagag ttttagaaag
     8761 gtaaatggga atagttttgc aacgacaatg tgtactggaa atttaagaag tgctacagaa
     8821 accttatttc attatataca tactaagaat atttttatga taaaaaagag tttacaatat
     8881 tatgctatta ttatattctt tatatttgga gctattttag gaactttttt tacaaaaatt
     8941 ttcgtggaaa aatcaatact aatatgtttt tttattttgg cagtggtatt tggaattatg
     9001 tttatcaata aagatgattc atttaatgaa aattaacttt ctgtaaattt acaaaaaaat
     9061 gaagc
//
LOCUS       DAMJVM010000123         8993 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.128, whole genome shotgun sequence.
ACCESSION   DAMJVM010000123 DAMJVM010000000
VERSION     DAMJVM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8993
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.128"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(343..1239)
                     /locus_tag="PC523_003153"
     CDS             complement(343..1239)
                     /locus_tag="PC523_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2476645.1"
                     /translation="MNDKNTYFDRKGKENFIDIFFKDDIKKGANFKRHWHEHLQVYYF
                     VDGIAYIECGLNNFKIVSGNIAIANSNELHYLESLSDNLKFYIIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSFILKCIKTILDEYHNKGIGYELSIKANIYQLIVL
                     LLRSHVDKIFSEDEFIERKYSLEKFEYIFDFIEEKYMDKITLKELASHVNLSVPHFCR
                     TFKKITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYDV
                     SPTKYRNEHREISNVILKEGIK"
     gene            1381..2808
                     /locus_tag="PC523_003154"
     CDS             1381..2808
                     /locus_tag="PC523_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2476646.1"
                     /translation="MTNSFKKDENKKRMIVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLSSIEWVIASYSIIICSTLLFFGRLGDIIDKSRVFQSGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKENRGKALGILVTAVAIGNMVGPSIGG
                     FILSIFDWNFIFFINIPIGIIVIFLNTKFLPNSKKSSENMNKTGAILQFLGTTLFFSA
                     LISAQQAGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQNTMKLAPIQAGSFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLFMSFGFFLMSRLKESSALILFVIYISIISMGQAIFQPANNALIMSSCYRSKL
                     GIVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYIINNDKIFVFGMRNVYT
                     IITLICLIGAILIGFYLFKYDKNKQ"
     gene            complement(2985..5579)
                     /gene="clpB"
                     /locus_tag="PC523_003155"
     CDS             complement(2985..5579)
                     /gene="clpB"
                     /locus_tag="PC523_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDF2476647.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSNRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETVIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(5616..6212)
                     /locus_tag="PC523_003156"
     CDS             complement(5616..6212)
                     /locus_tag="PC523_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476648.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIVKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(6229..6633)
                     /locus_tag="PC523_003157"
     CDS             complement(6229..6633)
                     /locus_tag="PC523_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HDF2476649.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            7000..7374
                     /locus_tag="PC523_003158"
     CDS             7000..7374
                     /locus_tag="PC523_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2476650.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            7371..8234
                     /locus_tag="PC523_003159"
     CDS             7371..8234
                     /locus_tag="PC523_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2476651.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWSDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHNPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            8234..8872
                     /locus_tag="PC523_003160"
     CDS             8234..8872
                     /locus_tag="PC523_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2476652.1"
                     /translation="MKGLILKDLLNLKGNVKFILLFIIMFGFMSSLGDGNINNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
BASE COUNT         3024 a         1328 c         1022 g         3619 t
ORIGIN      
        1 tttagagtgt atttaagttt tctataaaaa gcatactctt ttagctgttt acttacttat
       61 ttgatagttt tgtttcttta attaaatatc tttcagaatc taagctagat gtctcaaaaa
      121 aaacagattt gtaatataat tattttttct gctattccta tttttccaac cttagtttca
      181 tagtaaagta cacttttcat atacactaca ttctttcata ctcattttca aatttaacta
      241 cacaaatagt cttactcatt tatatcctat actttattaa tgtttattaa gaaaaactca
      301 aagatttaaa aatatatatt ttctataaag tatatttatg aattatttaa taccttcttt
      361 taaaataaca ttactaattt ctctatgttc atttctatac ttagttggag atacatcata
      421 attcctttta aataagcgac taaaataatt tatgctatca aagccgcatc tcatagcaat
      481 ttctgtcata tttaaatctg tttcctttaa ataatatata gctttatcaa gtctgattcc
      541 atttataaaa tcgattgtag tcttacctgt tattttctta aatgttctac aaaaatgagg
      601 cacactcaag tttacatgac tagcaagttc ttttaatgta attttatcca tatatttttc
      661 ttctataaaa tcaaatatat attcaaattt ttctaaagaa tactttctct ctataaattc
      721 atcttcagaa aatattttat caacatgact tcttagtaat aatactatca attgatagat
      781 attagctttt atagatagct catatccaat tcctttattg tgatattcgt caagtatagt
      841 tttaatacat tttaaaataa aagaatcatt ttgaattaaa ttattaaata caatatgatt
      901 taaagctaat ggtgctaagt acttagtttg taacaaatca acttgattac taaaaaggaa
      961 tgtgggttca attcgaataa tatagaattt taaattatct gataaacttt ctaaataatg
     1021 caattcatta ctatttgcta ttgcaatatt tccagatact atcttaaaat tattcaatcc
     1081 acattcaata taggctatac catcaacaaa ataatatact tgtaaatgct catgccaatg
     1141 tcttttaaaa tttgctccct ttttaatgtc atccttaaaa aaaatatcta taaagttttc
     1201 tttacctttc ctgtcaaaat atgtgttttt atcattcatt atttacctct tatcaaaata
     1261 gtgctaatta tgagcaatat cgttattgta ttcattattt cataccgata ttatataaga
     1321 tgtgactatt attgtaaaca ttaacatgat gccagtcatt ataataagga ggtaataaaa
     1381 atgacaaact cttttaaaaa agatgaaaat aaaaaaagaa tgatagtttt atttgtagtc
     1441 attgcaatga cctttatggc aacattagat tctagtatta taaatgttgc tcttccagtt
     1501 cttgcaagca aactaaatgt atctctatca tctattgaat gggtaatagc aagttactcg
     1561 attattattt gttcaacact attatttttt ggaagacttg gggatattat tgataaatcc
     1621 agagtatttc aatcaggtac tattcttttt acatcagctt cattattatg tggattaagt
     1681 aattctttaa ctttacttat agtttgtcgc tttattcaag ggataggtgc ttctgcctac
     1741 atggcaaaca atcatggtat tatcacagaa ctgtttccaa aagaaaacag aggaaaggca
     1801 ctaggaattt tagttactgc agttgctatt ggaaatatgg ttggaccatc tataggagga
     1861 tttattttgt caatatttga ttggaatttt atattcttta taaatattcc aatcggtatt
     1921 attgtaatct ttttaaatac aaaatttctt cctaatagta aaaaatcttc agaaaatatg
     1981 aataagactg gtgctatact tcaattttta ggaacaacat tattttttag tgctctaatt
     2041 agtgctcaac aagcaggatt actaaatcca tatatattaa ttgcactatt attatcaatt
     2101 atttttatta tattattttt gatattagaa aaaaaacatc ctcaaccgct tcttgacctt
     2161 gaaattttta gaaattttaa attttcattg aatttaattt gcgctcttac atcattcatt
     2221 tgtattgcat catctagtat tttaattcca ttttatttac aaaatactat gaaacttgcc
     2281 cctattcaag caggttcatt tatgatatta tcacctttga ttttagccat tttttcacct
     2341 atatttggga atatatcaga taaaattaag tcagaaaaaa ttattttaat tggtcttctc
     2401 tttatgagtt ttggattttt tctaatgtca agattaaaag aaagttctgc ccttatttta
     2461 tttgtaattt atatatcgat aatatcaatg ggacaagcta tatttcaacc tgctaacaat
     2521 gctttgataa tgtcaagctg ttatagaagt aagttaggaa ttgtaggaag cataaattcc
     2581 cttgtaagaa atttaggaca agttataggt attactattt ctacaacact tttatataat
     2641 tttatgagta ttaaagctgg atatagggtc aatgattata taattaataa tgataaaata
     2701 tttgtatttg gaatgagaaa tgtttatact attattacac ttatttgcct aataggtgca
     2761 atattaattg gcttttattt atttaaatat gataaaaata aacaatagtt tttttataat
     2821 tgtattcaaa ccattattat attataagaa acttactaaa atactccctt tgagttaaat
     2881 ttaatttgaa cacattggct taaaaatatt tcttttccta tagtttataa ataaaaatca
     2941 gctgagcaaa atatttcata tactatgctc agctgattct cttattattt aactaaaact
     3001 tcaatatttt catcttttcc atctataact atagtacatc cattatacac atctccagca
     3061 ataagtttct ttgcaattac agtttctaat gtatttccta tatatctctt aagaggtctt
     3121 gctccgtata cagggtcata tccctctctc accataacat cttttgctga atttgtaact
     3181 tcaatagtaa tatctttttc ttttaatcta ttttttaaat ctttcataaa tatatctata
     3241 attttcttga tattttcttg gtctaaaggc ttaaacatta taatatcatc tactctattt
     3301 aaaaactcag gtttaaatct atgcttcatt tcattcatta ctaaattatt agtagtctct
     3361 gttatattcc ctcctgcttc taatagataa ttacttccta tattagaagt cattattata
     3421 agagtatttt taaagtctac tgtcttaccc ttattatcag taagcctacc atcatctaat
     3481 atctgtaaga acatgttaaa tacatcctca tgtgcttttt ctatctcatc aaataatatt
     3541 acagaataag gagcacgtct aactgcttct gtaagttgac caccttcttc atatcccaca
     3601 taccctggag gaggcccaac tagtcttgat acagcatgtt tttccatgta ctcagacata
     3661 tcaattctga ttatattatc ttcactatca aataaatttc tagcaagagt ttttgcaagc
     3721 tcagttttac caacacctgt aggacctaag aaaataaatg aaccaattgg ttttctttca
     3781 tcttttaatc ccgctcttgc acgtattact gcatttgaaa ctgctgtaac tgcttcatct
     3841 tgtccaataa ctcttttatg aagttcatct tctagtttaa gtaatttttc tctttctcct
     3901 tcaactagtt tagttactgg tatacctgtc cactttgata caatgctaga tatttcctct
     3961 tcagtaactt cttcttttaa aagagaattt tcagaaccat cgttcatatt ttcttcgtac
     4021 tgctttattt gttcttctaa cttaggtatt tcaccatatt taacctctgc tgctttatta
     4081 aaatcatatt ctctctcata tttttcagct ttacctttag cttcgtctaa ttgagccttt
     4141 aagtttttaa tatctaatat ttgactcttt tccttttcat attttgctgt catttcatca
     4201 tttttagatt ttaactctgc tatttctttt tgaatatcat ccaatcttga tttactttta
     4261 tcatcatttt ctttaagtaa tgcttccctt tcagtttcca atgtaaataa ttttcttcta
     4321 acaacatcta actctgttgg taatgaatct atttcgctac gaatcatagc tccagcttca
     4381 tcaattaagt caattgcttt atctggtaaa aatctatctt gtatatatct attagaaagt
     4441 tttgctgccg ctacaatagc attatcatgt attcttactc catggtgtat ctcaaatctt
     4501 tcttttaaac ctctaagtat agatattgta tcttcaacag taggttcctc tgcaattaca
     4561 ggttgaaaac gtctctcaag agccttatct ttttctatat actttctata ttcatcaaaa
     4621 gtagttgcac caatacagtt taattcacct cttgcaagca taggcttaat aagattgcct
     4681 gcatccattg agccttctgt tttaccagct ccaactatag tatgaatctc atctataaat
     4741 agtataattt ttccatcaga actttgaact tcttttaaga ctgcctttaa tctttcttca
     4801 aattcacctc tatattttgc accagcaatt aaagcaccca tatcaagtga gaatacaatt
     4861 ttatctttta aaccttctgg gacatctcct cttacaattc tctctgctaa accttctaca
     4921 attgcagttt tacctacacc aggctcacca attagcacag gattattttt agttcttctt
     4981 gataaaattc ttataattct tcttatttct tcatctcttc caattacagg gtctaattta
     5041 tttttcttgg ctaaatctac taagtttgtt ccatatctag caagtgcatc atatgttcct
     5101 tctgggtctt gtgtttctac tctttgacta cctctcactt gtgataaaat gtttaaaaag
     5161 tcattcttat ttatattata ttgctttaat attttaccaa cagctgattt tgattctgtt
     5221 tccatcattg caagcataac atgttctaca cttatatatg agtctttaaa ttctttagaa
     5281 atgctctctg cctttataag cacctcattt atttttctag tggctgatac tccttgtgag
     5341 tcagctcctt ctccatatac ttttggaagc ttatcaattt caaagtttac agtatttctc
     5401 aaagaatcaa ttgatatatt cattttttcg attatatttg gtattaatcc atcttcttga
     5461 ttaataagtg ctgagaaaag atgaattaca tctacttgtt ggttatgatt ttttactgct
     5521 tcattataag cctcatttaa acttttttga actcttaaag tcattttttc tacgtccata
     5581 taaatccctc cattttaata ttatatccaa ataaattaaa ttctctcatt tataattatt
     5641 gattttctaa gctctaaaag actatcaagt aaattctcat ttttttgtat atcgaaactc
     5701 aaagttaaat taaatgttcc atctgaatta aaaaaatcaa atacttcttt tgattttact
     5761 attaaataca aatcttcatc tataagtctt ttttcaccta ttaataaata ctgacattta
     5821 tctataaagc tctttttaaa atcaatagtg tatagatttt ctaaaatatt atattgataa
     5881 atggcaatat tgttaaacac ttttaatatg tttgaattta caaaattgta aatttgatta
     5941 ttgttataca gtgatatttg ttcgtatata tattccctaa ataaaggatg aatgttttgc
     6001 ttaagtttta aagtaacttc agctggtatt tcaaaataaa gcttccagta cttagagtta
     6061 tttttttcta aaataaaagc tactttatta agactggtag aactaatctc ctttagtgta
     6121 caattaaaat tatagtgtct caaactagtt ttttcattct caaatacata atcaaaccac
     6181 tgtagaacat ttctatcaag ttttaaactc atataaaaac cttcttcctt atttactaac
     6241 cttattaggt ctttttatct gattatattc tgctaataaa ctttctttat cagctctact
     6301 acctttatat gctatatgtg gatttatcat atactcacca tttttcactc tttgtaaaaa
     6361 atccattttt ttcagctccg taaacaatga agataaagaa gtttttggat attcaaattc
     6421 acaagataat tcatataatg tttttctaat cttattattt ttatccatag agtttattaa
     6481 ttttaatgga attaaatccc tcttagtata cattccttct actactttaa tataattctt
     6541 aaaacaaaca ttcatatact tacttttatt cataactaaa cttttactct ctgtgtattc
     6601 ctgatttaaa tcctttttta atgtcttatc caaattaaat atctcttctc actctcctta
     6661 tctctaattg ataatataaa tatattacaa aaaaatatgt atgtcaaaac tgaaaacaca
     6721 aaaagtttag cttataaata aacttttatt tacctataag ctaaacttta ttattttttt
     6781 aatttagtat ttccaattta ttggcaggat ttttgaataa tatttataaa ttttttaaaa
     6841 aaatttaaat ctatttaata tggtagcctt tcataaaata cattacattt attaaatgta
     6901 taatattggc aattagtttt ttctaaaatc agttgacata ttatatatat atttatataa
     6961 tgtgtatata ctatatatac aatttaagga ggtgtcttat tgaatatagt tattagtaat
     7021 tcgagtggga agccaatata tgaacagatt actacacaaa ttaaaagtat gattataagt
     7081 ggagaattac cagaaggctc agctcttcca agcatgagac tattggcaaa agaacttaga
     7141 ataagtgtaa ttactacaaa acgagcatat agcgatttag aacgagacgg atttattgag
     7201 acagttactg gtaaaggcag ttttgtatct agtaaaaaca tggattttgt caaagaagaa
     7261 caatttcgtt taattgaaga atacctacag aaagcagttg atacagcaaa aagtagtgat
     7321 attacatatg acgaattaaa agatatttta tttttattat ataaaggaga atgataagta
     7381 tggatgcaat aactataaaa aatttaaata aaacttataa agattttagc ctacaagata
     7441 tatctttttc agtaccaaaa gggagtgtaa tgggatttgt aggtgaaaat ggtgcaggaa
     7501 aaacaactac attaaaagca attttaaatt taatatctta tgatagtgga aatatagaaa
     7561 tctttggtct tgatagtaaa aaaaatgaaa aagaaattaa agagcaaatt ggggttgtat
     7621 ttgagggaag caactttcat gaaaatttaa acactgacca tgtttcaaaa atcatgagta
     7681 aaatctataa aaattggagt gacacattat ttaaagacta cttaaaaaaa ttgagagtac
     7741 cagacaataa attaattaag gagttttcaa aggggaataa gatgaaactt agtattgctg
     7801 tagccttatc tcataaccca aaattattaa tactagatga ggcaactagt tcacttgacc
     7861 ctatcgttcg tgaagaaata ctagatatat ttttagattt tatacaagat gaagaacact
     7921 ctattatctt atcttcacat attacaagtg atttagataa gatagcagac tatattacat
     7981 ttatacataa aggaaaaatt gtatttagtg aaaataagga tgaattaatt gatactatgg
     8041 gagttttaaa atgtaaacct agtgattttg ataacctttc aagagaggat tattcatatt
     8101 atcgtaaaag ccaatttgga tatgaagtac ttcttaaaga taagcataga tttatctcaa
     8161 gacatccaaa ctgtattgtt gataatacat ctatagaaga aattatgtta ttttatgtga
     8221 gaggtgataa ataatgaaag gtctaatttt aaaagattta cttaatttga aaggaaatgt
     8281 aaagtttata cttttattca tcattatgtt tggttttatg tcttctttag gagatggaaa
     8341 tattaataac tttataggtg ttattatagt tttatgtact acaatgattg tttcaacatt
     8401 ttcatatgat gatttaaata aatgggatag ttatgtactt acaatgccta ttaatcgcaa
     8461 tgatattgta ttaagtaaat atttaactat gttaatattt agcttcatag gtgttctcgt
     8521 ttcacttatt gttagtgtaa ctattggtta ttttaaaaat acacttatat taaatgaaac
     8581 actacttatt aatgcactaa tcttatcaat ttcagtatgt tttggtagcc ttattttgcc
     8641 acttatttat aaatttggta cagaaagagc tcgtttatta atgattttat gctttttagt
     8701 tccaacacta gctttattag tttttaaaag tattttagaa aatattagtt cacctatttc
     8761 tatagaaatt atacttaata cactagtata ttctcttcca tttgttgcta ttttactatt
     8821 tgtgatttca tattttatat catcaaaaat ctatagtaaa aaagaagtat aaaatatact
     8881 atatatgttt ctgtatttaa gtatttaaat aaaatactat cgattttatt ttggtagtat
     8941 tttatttttc aattattaat ttctactagg tatttattat tttattactt ata
//
LOCUS       DAMJVM010000124         8946 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.129, whole genome shotgun sequence.
ACCESSION   DAMJVM010000124 DAMJVM010000000
VERSION     DAMJVM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8946
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.129"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            <1..339
                     /locus_tag="PC523_003161"
     CDS             <1..339
                     /locus_tag="PC523_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2476653.1"
                     /translation="KENDIICIEDLQVKNMIRNHKLSRLISDVSWSEFIRQLEYKANW
                     YDRQIVKVGKFFASSQICNKCGYKNEEVKNLNIREWICPSCNETHDRDINASINILKE
                     GLRLITIQNK"
     gene            457..1644
                     /locus_tag="PC523_003162"
                     /pseudo
     CDS             457..1644
                     /locus_tag="PC523_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            complement(1792..2667)
                     /locus_tag="PC523_003163"
     CDS             complement(1792..2667)
                     /locus_tag="PC523_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2476654.1"
                     /translation="MINIRYLEILEAIEQTGTFTGAAKKLHITQSAVSHAIAELEQQA
                     ETSLFDRLPRGVCLTRCGISLLEEARSILTACRNLDKRISRLEECTPINIVSSITIAS
                     FLLPPILSRLKNLFPSLKVNVRVESANSAMDILKSGEADICFWEGIEPQGAFRTILLG
                     SYKLCVACAPTFPLSEQTLSLRQLCTFPLLLREQGSAIRDTLDSVLSLSNQKAYPIWE
                     SVNSFALIKAAEAGLGITILPENLLLDSLLHKKLRLIELDGIDMENKMLAILHKDKNI
                     TQPLKIILDNISNVL"
     gene            2787..3350
                     /locus_tag="PC523_003164"
     CDS             2787..3350
                     /locus_tag="PC523_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2476655.1"
                     /translation="MNQSRKTYFWLLIINLFISTFTFGGGYVVVPMIRRYFVNQKHFF
                     TEDDLMSMAAVAQSTPGAIAINLSALAGYRVAGTLGVVISCIAAIIPPLVILGLVSAF
                     YTTFISNAIVTAVLKGMQAGVAALIVDLIIDMCSMILKQRSLFLSAMIPIAFFANFVM
                     SINVIFILSVCCCVCVMQVFYKKRKNK"
     gene            3347..3913
                     /locus_tag="PC523_003165"
     CDS             3347..3913
                     /locus_tag="PC523_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2476656.1"
                     /translation="MNTIWNLFFIFLKIGLFSIGGGYAIIPLIQEKVVNQTGWISEKM
                     FTDIITISQMTPGPLAVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKH
                     QNSTYIFEILNGLKSASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFV
                     TLLIVRKWKINPIVIIIISGILGIFFYL"
     gene            complement(4042..4875)
                     /locus_tag="PC523_003166"
     CDS             complement(4042..4875)
                     /locus_tag="PC523_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2476657.1"
                     /translation="MSWKHIKIPNGDTMIFCNSFITSKLSMNIIVTHTPIVTTLEMQK
                     AYEPFTKYNVNIFAFDFSGTGKSDGYEKDFSRNSIIKDLDSIITYIEKNYSSNIHLYG
                     NTGIGGMFAQYYACSDNKLKSFAQFACVDYKNTTGVGYPYLTVKLLNLFFKFLPNFHI
                     TVKPPKYKGYNEVYDNEFYKIFEEKNPHIWKNSTKIINTMLECFIAEDSKIKDGVNIP
                     TLVFKTLHDRYFKTQYFDSYYNSLKCKKKLVEINNVHNSYYIDSEKFCQEAYDWFVEN
                     Q"
     gene            complement(5060..8488)
                     /gene="uvrA"
                     /locus_tag="PC523_003167"
     CDS             complement(5060..8488)
                     /gene="uvrA"
                     /locus_tag="PC523_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2476658.1"
                     /translation="MIINNYQNQSIITNPKKFENEYQYLPDTPIELLKVVQNLIIHGD
                     QGKLYGISFNKQQSDEELLRTVPQMLKRIFEINSNSLTIPRTPKQRLVGMCRDYSLLL
                     VSFLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTATKRWIKIDAQIDDIQKNYF
                     QINFDAHDVRRIDGFLTGSEAWIKCRQEHTHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNKKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELANPLELEKKFKFTKAINKPIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNKITVITGVSGSGKSSLAFDTIYEEGKRRYFENLGNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGNQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKTPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESRGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELTNQLQQKELSLVNDILTELKIRVSYLLK
                     VGLSYLSTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPNAGIKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPNKNYTELTGFDAIDDFILMDQTAIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYIADQWVTCNKC
                     QGKRYKKEILSIQYKGKAIADILDMEVAEAKTFFSDCSDIYRKLYLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGMNGGEIVASGTPDEIKVNPNSITGHFI"
BASE COUNT         3027 a         1265 c         1382 g         3272 t
ORIGIN      
        1 aaagagaacg atattatttg catagaagat ttacaagtaa agaatatgat tagaaatcat
       61 aaactatctc gcttaatttc agatgtgtca tggtcagagt ttattcgtca attagaatac
      121 aaggctaatt ggtatgatag acaaattgta aaggtaggta aattctttgc aagttcacaa
      181 atatgtaata aatgtgggta taaaaacgag gaagttaaga acttaaatat aagagaatgg
      241 atttgtccta gttgtaatga aactcacgat agagatataa atgcaagtat aaatatacta
      301 aaagaaggat taagactaat aacaattcaa aataaataac ttatgtaaga accgtaggaa
      361 ctatggggat agcctgtgga gaattagtaa gacatatttt agtatgcaaa attctatgaa
      421 gcaggaaccc tgtgacttta gtcatgggag gttcagagtg gggaagtatc tggttctagc
      481 atggatagtt attttagtgc taggaataag gatattgata gtaacactga gcgttacatt
      541 gagacgccta tttcaaaaga tatgaaagag gtaggttatg atgttgcagt tcttgggaat
      601 catgaattta tatccaacaa taaattttat ctggataata tgatatctga ttttgagaag
      661 caaaaaataa atattttatc agcaaatact tacaaaaaaa atggagacag ttatacaaaa
      721 ccatacataa taaaaaatat taatacacct gaaggaaacg taaaactagg aattttaggg
      781 cttactataa aagaagttgg tgagaggaaa cagtggaaag atggaaaact tgttgactct
      841 aagtctttag aactaaaaga ccaagagggc tacaatggtg aattgtatat gaatgattta
      901 gtagaagatg ctaaaaagtg ggttagtata atggaaaaag agaagcctga cattatagtt
      961 gctgtggcac atacaggtga gaaacctaag aagcctagaa acccaggaaa tagaatacaa
     1021 gatttagctc aacaagttga tggaattgat gctatagttg caggtcataa tcatgttcaa
     1081 attgagcaac atgattataa aaataaatca ggagaaaatg ttatagtcac tgagccagga
     1141 aaacatggag aatgtatttc taagattaat tttaaattag aaaaaaccaa tgataaatgg
     1201 aatatagttg acaaatctag taaattgatt aaatttgaaa aaaatcaaga aagtgacaaa
     1261 tttggagaat ttatttcttc aattaataaa gtaagtaaag ataagaaaga aatacgatta
     1321 tcagaagtaa agactcctga atgggataaa atatatgttt ttaaatctgg aacatctgca
     1381 gatgatatat ataaaacaat aggatataaa tggagaaata tatcatctcc aaagggtgat
     1441 gacatggtgc agattgttgt aatgaataat aataagcctg ttagctatgt atatggtgaa
     1501 gctgaagtta tgccaattaa tataaaatgt gatgaatcag agtataaaga taatgttgta
     1561 actatttatc ctaataaaaa tgataaattt aaaattgaaa aaggtaaaca agaatatagt
     1621 attaatttaa aacatataca atagaaactt ttatcttatt taagtttaga ttgtacttaa
     1681 aataaagtgt gtattatttt aaaaagtgat tataattaaa atcacaacat taaggaatag
     1741 tacacacttt ttatgctaaa aaatagaatg ttaaaaaatt aaatctttaa attaaagtac
     1801 attgcttata ttatctaata ttattttcaa aggttgtgta atatttttat ccttatgtaa
     1861 tattgccaac attttatttt ccatatctat cccatctaat tcaatcaatc gcaatttttt
     1921 atgtaagagt gaatctaata ataaattctc tggcaaaatg gttataccta accctgcttc
     1981 tgcagcttta ataagtgcaa aagaattaac actttcccaa ataggataag ccttttggtt
     2041 tgaaagagat aatacactgt ctaaagtatc gcgaatagca cttccttgct cacgtaacaa
     2101 taacggaaac gtgcataact gtcgtagaga taaggtttgt tcagataaag gaaaagttgg
     2161 agcacatgca acacataact tataggaacc taataaaatt gtacggaatg ccccctgtgg
     2221 ttctatacct tcccaaaaac atatatcagc ttctccactt tttaaaatat ccatggctga
     2281 attagcactt tcgacacgta cattcacttt aagagatggg aacaaatttt ttagtcgact
     2341 taaaattggt ggaagtaaaa aagaggcaat tgtaatgcta gaaacaatgt ttataggtgt
     2401 acattcctct aaacgactga ttcttttgtc taaattgcga caagcagtta agatactgcg
     2461 tgcttcttct aaaagagaaa tgccacagcg agtaagacat acccctcgtg gtaatctgtc
     2521 aaataatgat gtttcagctt gttgttcaag ttcagctata gcatgagata cagcagattg
     2581 tgtgatatgt aatttttttg cagcacctgt aaaagtacct gtttgttcaa ttgcttctaa
     2641 aatctctaaa tatcgtatgt ttatcataat aaatatcctc actagacatg aataaaattg
     2701 attaattcca ttaaattcta tcattttaca gattgcaaag caacaggtat actgaataat
     2761 atgaagtgaa aagaaggagg aatattttga atcaaagcag aaaaacatat ttttggcttt
     2821 taattattaa cttgtttatt agcacattca cttttggtgg agggtatgta gttgtaccta
     2881 tgattagacg ttattttgta aatcaaaagc atttctttac agaagatgat ttgatgagta
     2941 tggcagcagt tgcacaatca actcctggag ctattgcaat taatttatct gctttggcag
     3001 gttatcgagt agctggtact ttaggggttg tcattagttg tatagcagct ataatacctc
     3061 cactcgttat tcttggatta gtatcagcat tttacaccac ttttatatct aatgctattg
     3121 taactgcagt attaaaagga atgcaagctg gtgttgctgc actaattgtg gatttgatta
     3181 ttgacatgtg ctctatgata ttaaaacaac gttctctatt tttatcagct atgataccta
     3241 ttgctttctt tgcaaacttt gtgatgagta taaatgtaat ttttatatta tctgtttgct
     3301 gttgtgtatg tgtaatgcaa gttttctaca agaagaggaa aaataaatga atacaatttg
     3361 gaacttattt tttatatttt taaaaatagg cttgtttagt attggtggtg gctatgcaat
     3421 cataccttta atccaagaga aagtagtaaa tcaaactgga tggataagtg agaaaatgtt
     3481 tacagatatt ataactattt cgcaaatgac accaggacca ttggctgtta atacttctac
     3541 ttttgttggg cttcaaattg gagggttttg gggagcagtt gcagccactt taggttgtgt
     3601 attatgtggt gtagtaatat ctctattttt atataatttt tttcaaaagc atcagaactc
     3661 tacttatata tttgaaatat taaatggtct aaaatcagct tcattaggtt taattatctc
     3721 agctgcggct acaattataa tccttgcatt atttggtacg aataaagtgt acttcaattc
     3781 tatatttgag atgctaaact ggactgcact aataatcttt tttgttacgc tactgatagt
     3841 acgtaagtgg aaaattaatc ctattgtcat tataataata agtggtatac tgggaatatt
     3901 tttttattta taagtttttg ataagtaaag gatatctatt tttttatatc ttaaatccaa
     3961 atagccaata aagtaaggct atttggattt ttaataaatt ctgaaaagat aatcagaaat
     4021 ccatataaca tttagattat atcactggtt ttctacaaac caatcataag cttcttggca
     4081 aaatttctca ctatctatat agtaactatt atgaacatta ttaatttcaa caagcttctt
     4141 tttacatttt aatgaattat aatagctatc aaaatattgg gttttaaaat atctatcatg
     4201 aagtgtttta aagacaagag taggtatgtt gactccatct tttatcttac tatcttctgc
     4261 aataaagcac tctaacattg tattaattat ctttgtacta tttttccaaa tatgaggatt
     4321 tttctcttca aatattttat aaaattcatt atcatagact tcattataac ctttatattt
     4381 aggtggtttt actgttatgt gaaaattagg aagaaactta aagaaaaggt ttaataattt
     4441 aacagtgaga tagggatagc ctactcctgt tgtgttttta taatcaacac aagcaaactg
     4501 agcaaaactt tttaacttat tgtctgaaca tgcataatat tgcgcaaaca tgccaccaat
     4561 ccctgtattc ccatatagat gtatgttgga agaataattt ttttctatgt atgtaatgat
     4621 agaatctaag tcttttataa ttgaattgcg agaaaaatct ttttcatatc catcactttt
     4681 tcctgttcct gaaaaatcaa aagcaaatat atttacatta tatttagtaa atggttcgta
     4741 tgctttttgc atctcaagcg tagtgacaat aggagtgtgt gtaacaataa tattcataga
     4801 taatttgcta gtaataaatg agttacaaaa aatcattgta tctccatttg gtatttttat
     4861 atgtttccaa ctcatttgat tttcctttct ctattataaa acataataat tcttatactc
     4921 tattttatag attattcata ttttatcaca aacaatatac taaagttaat ttttttatta
     4981 tactactatt ataataattc ttccataata taagatattc tataatttta tgtctaagtc
     5041 catgtttatt tagttgtttt tatataaaat gtccagtaat actatttgga tttactttta
     5101 tttcatcagg agttccagaa gcgacaattt cccctccatt cattccactg tctggaccta
     5161 tatcgataat ccaatcagaa gcacgtataa cctctgtgtt gtgttcaata attaaaagtg
     5221 aatgttgctc atttacaagt tttctaaaag taattaacaa attttctata tctttaaaat
     5281 gaagtccagt agtaggttca tctaatatgt ataacattcg ttttttagtt tttgttccca
     5341 attcttttgc taattttatc ctttgagatt ctcctccact taatcctaat gtattttgtc
     5401 caagctttag atagccaagt cccacttcat ctaataagta gagttttcta tatatatcag
     5461 agcaatcact aaaaaatgtt tttgcttctg ctacttccat gtcaagtata tctgcaattg
     5521 cttttccctt gtattgtata gacagtattt cttttttata tctcttacct tgacatttat
     5581 tacaagttac ccattggtct gctatatatt gtagaataat ttttgtttta cctaatcctt
     5641 gacagtttgg gcattgcccc tttttgctat taaagctaaa atagctttca tccaataatt
     5701 tttgttttgc ttgtggagtt tcagcaaata gagaacgaat ttcatcaaaa atattgatat
     5761 atgtagcagg tgtagaacgg ctagattttc caatagctgt ttggtccatt aagataaaat
     5821 catcaatagc atcaaatcca gtaagttcag tataattttt atttgggata gatttttgtt
     5881 ttaatttttc ttctagagca ggaagtaaac tgtgaaatac aagagaactc ttaccagaac
     5941 cactaacacc tgtaatagag cacatacagt ttaagggaat ttctacatca atatttttta
     6001 agttatttgc atggcatccc tttaagctta accatttact tggtataatt ttattttgtg
     6061 aacttatctt attgtatgta ctcaaatatt tgcctgttat agaatttgga ttttttataa
     6121 tttcttgggt tgtaccttct gcaatgatga agcccccttt tataccagca tttgggccta
     6181 catcaataat ataatcagca gacagtattg tatccttatc atgttccact actataacag
     6241 tattaccctt atctcttaat tcttctatca ttttgatgat tagattatgg tctcttgggt
     6301 gtaaaccaat agatggttcg tctaaaatat aggttaatcc tactaattca cttcctagct
     6361 gacttgataa tcgtactctt tgtagttctc ctccagataa ggttggagct gttctatctg
     6421 ttgataaata actaagacct acctttaata gataactaac tcgaattttt aattctgtca
     6481 atatatcatt tactaaagat agctcctttt gttgaagttg atttgtcaat tcacacaacc
     6541 aattccataa ctgttctatc gtcatttttg ttagttcagg gaagcgataa cctttgattg
     6601 tagtgaacct agcttcaatg cacagcaatt cgccaccaca tgtattgcaa ggaattttgt
     6661 tcatatattg atgcagggta ttattttcag aactagattc tctaaaaagt ctttgaatgt
     6721 ggttgattgc tcctgaagca ggtcttctaa tctttgattc tcgtcccctt gattcaaaac
     6781 taaattcgta tatcttatcg tctgtaccat ataagattgc atctataaaa tctcttggca
     6841 attcattcca aggtatatct aggtctatat ttttatcttt tgctattgca tataattcac
     6901 ctaccatcca attaccattt ggctttttcc ccctcaattt tccaaaatag taagtagctc
     6961 catctaatat tgaaatagtt gggtctttta caattaattg aggattaact tgataggtaa
     7021 aacccaatcc attacaatcg tgacaagctc cagtatggga gttagcattg aaggttgaag
     7081 tatttatctt agaaaaaata gtttggcaat gtggacaata attttttgta ttattctttt
     7141 tttctaatgg agttttgcaa tatggacagt aactttttcc gattgataca aataacattc
     7201 ttaagtagtc ccaaatgctt gtaagagtac caacagtaga acgaggattt tggtttcctt
     7261 ttttctgttc gatagctata gtaggggtta atccctgaag taaatcaaat tccggttttt
     7321 gaagctgttc tgaaagtttt gctccattac ctagattttc aaaataacgt ctttttcctt
     7381 cttcgtaaat cgtgtcgaaa gctaaagaag attttccact accacttaca cctgtaatca
     7441 cagttatttt attctttgga attgtaactt caacatcttt taaattgttt tgtcttcctc
     7501 cttttaaata gatagagctt ttattatgac aaagtgaatt tttaattggt ttatttattg
     7561 cttttgtaaa tttgaatttc ttttctagtt caagaggatt agctaattct tttatttccg
     7621 aaatacctaa tatttttaac tgagaaaaga ttgatttcaa ataatcttca ggcaattttt
     7681 ctattaacaa attaaggtct tctatttttt tatttccgct tgataatatc tctgccattt
     7741 catctaataa atttttttct gctcttgtta attgattgta ttttttggaa cttaattctg
     7801 tccataaatc ccatggaagt aattccaaac caatcatatt attaaagtca tgtagaagat
     7861 taccctttac agaatgccaa cctttccaat ttttattata tccaaagtca tctggatgag
     7921 tatgctcttg tcgacatttt atccaagctt ctgaaccagt taaaaagcca tctattcttc
     7981 tgacatcatg agcgtcaaaa ttaatttgaa aataattttt ttggatgtca tctatttgtg
     8041 catctatttt tatccatctc tttgttgcag tatcatagta ttctacaagc cagtggtctt
     8101 cataagtaag ttcactttca aaataattag caaaacctgc tctcattcta gcttcaaaac
     8161 ctcgatatct taggaaggac acaagaagta aagaataatc tctgcacatt ccaacaagtc
     8221 tttgttttgg ggttcttgga atagttagtg agttagaatt tatttcaaat attcttttta
     8281 acatttgagg aactgtacga agcaattctt catcagattg ttgcttatta aaggatatac
     8341 cgtatagttt tccttggtct ccatgaatta ttaggttttg aaccactttt aataattcaa
     8401 ttggagtatc tggtaaatat tgatattcat tttcaaattt tttaggattt gtgatgatac
     8461 tttgattttg atagttattt attatcattt atatttcacc tctctaagtg gatgatatat
     8521 cctgacatgg tgttatatgc aagggcaatc taaatttttc ggataaaatt taaaaataaa
     8581 taaaaaatga tacaatgata aattgtaata caaatatagt ttattttata tttgctgtag
     8641 taagaataag agctatttgt ataaaaaatg gatgatagat ttaagttttg taaaactaca
     8701 actactcaag ttaaaaaatt agaaatgatg tttggatagg tggacacatc atttcaaaat
     8761 caatactatc taatactata gattatgata taattgccat attattagag gagataagca
     8821 gtagctattg taatagattt tttatagaat atcgaaggtg ttattaaaat aaagaactat
     8881 accaaattta ggtatagttc tttattgata tatgacatgt cataatgttt ttaatcaata
     8941 aatatt
//
LOCUS       DAMJVM010000125         8788 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.130, whole genome shotgun sequence.
ACCESSION   DAMJVM010000125 DAMJVM010000000
VERSION     DAMJVM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8788
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.130"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            127..894
                     /locus_tag="PC523_003168"
     CDS             127..894
                     /locus_tag="PC523_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HDF2476659.1"
                     /translation="MQIIDININNIDNEHICCSISEKKGENCVFSKKLWMKNNFDKGL
                     VFKRFDVRGKAFIEYIPAENAWCPIIADNYMFINCFWISGKFKGKGYSNLLLQECVDD
                     AKLKGKYGLVVLSSSKKMPFLSDAKYLKYKRFRLADTSVPNYELLYFPFTDNTPIPCF
                     KNCVKQRKLNENGIVLYYSNQCPHTEKYALILKKIANSRGIDANLIKFETANEAQNAP
                     APFTAYSLFFNGEFVTNEVLSEKKIEKFLENEVKKFN"
     misc_binding    1113..1326
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1572..2606
                     /locus_tag="PC523_003169"
     CDS             1572..2606
                     /locus_tag="PC523_003169"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDF2476660.1"
                     /translation="MSKIKVGIIGATGYVGAELIRLLINHDKAELTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDVCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKDDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKSNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCQEKVSVVFTPNLIPVNRGILSTIYCTKADNISINDIHRKLTDYYEFKEFIEVL
                     PLDKVASLKNVRFSNKCVISLHENVDTLIICSAIDNMIKGAAGQAIQNMNIMFGIEEN
                     TGLKNIVPSF"
     gene            2647..3864
                     /gene="argJ"
                     /locus_tag="PC523_003170"
     CDS             2647..3864
                     /gene="argJ"
                     /locus_tag="PC523_003170"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HDF2476661.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     KIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            3879..4739
                     /gene="argB"
                     /locus_tag="PC523_003171"
     CDS             3879..4739
                     /gene="argB"
                     /locus_tag="PC523_003171"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDF2476662.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFKEGIITGGMIPKIQGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            4769..5956
                     /locus_tag="PC523_003172"
     CDS             4769..5956
                     /locus_tag="PC523_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDF2476663.1"
                     /translation="MNNNSTISKWTEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYAGEKIINASGMDKIYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLELVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKIKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYEEIDEALEILKDILL
                     EIN"
     gene            6066..7088
                     /gene="argF"
                     /locus_tag="PC523_003173"
     CDS             6066..7088
                     /gene="argF"
                     /locus_tag="PC523_003173"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDF2476664.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVADTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGGIMLTE
                     NIADVKGADVIYTDVWVSMGEEEQYETRINQLKYYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTDEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            7215..7832
                     /locus_tag="PC523_003174"
     CDS             7215..7832
                     /locus_tag="PC523_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HDF2476665.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTINSFFNSAKKFDFEGM
                     NKVMENNDEKYNDILKELDTKDPNEQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMLGMSFSGQDLTDEKTIEMLVSIMKEKQKSVKETYVTK
                     TVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSINALE"
     gene            complement(8026..8724)
                     /locus_tag="PC523_003175"
     CDS             complement(8026..8724)
                     /locus_tag="PC523_003175"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDF2476666.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSIPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKKSINI
                     PLLNIVEETVKKLPTLSQKVTLFSTSSTFESTIYQNGIIHNEHEFIFKDEWQIKLNNL
                     IQNIKIDKENPHNINIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
BASE COUNT         3525 a          838 c         1591 g         2834 t
ORIGIN      
        1 attcaaataa ttttaaaata atattatgtg aaataaaaat atatatgaca taatgttgtt
       61 gtattcaaaa tgttaaaata aagaatatat ttaaaaatta tcatgtaatt agaaaaggag
      121 ataagtatgc agattattga tataaatata aataatattg acaatgaaca tatatgttgt
      181 tcaatttctg aaaaaaaagg agaaaattgt gttttttcta aaaaactttg gatgaaaaat
      241 aattttgata aaggtctagt ttttaaacga tttgatgtaa gaggtaaagc ttttatagaa
      301 tatattccag cagaaaatgc atggtgtcct attattgcag ataattatat gtttatcaac
      361 tgtttttgga tatctgggaa atttaaggga aaaggatatt ctaatctatt gctacaagag
      421 tgtgttgatg atgcaaaatt aaaaggaaaa tatggattag ttgtgctttc gtctagtaaa
      481 aaaatgcctt ttttatctga tgctaaatat ctaaaatata agagatttcg tttagcagat
      541 acatcagtgc caaattatga gctattatat tttccattta ctgataatac tccaattcca
      601 tgttttaaaa attgtgtaaa acaaagaaaa ttaaatgaga atggtatagt tttatattat
      661 agtaatcaat gtccacatac agaaaaatat gctcttattt tgaaaaaaat tgcaaattct
      721 agaggtattg atgcaaatct tataaaattt gaaacagcaa acgaagcaca aaatgctcct
      781 gcaccattta ctgcatacag tctttttttt aatggtgaat ttgtaactaa tgaggtactt
      841 tctgagaaaa agattgagaa atttttagaa aatgaagtaa aaaaatttaa ctaaaaggag
      901 atataaatat actattttga aattatatta ttttataatt taatgtattt tgaaagtttc
      961 ttaagttgac taatgataat ataaaactta tatcacaatt tgaatgcaaa atggttaaag
     1021 ttaaccatat agaaaatatt tccaaaaatt tataaattat attgacaaac ttaaaaatat
     1081 aaactattat ataaaacaat aagaaaataa attgaaatcc tgtgaaaaag agagtagcat
     1141 aaaactaatt gatagagagc tgggaatggt ggaagcctag taatgaattt tattgtgaat
     1201 agagctttgg aggagattac cagaagtaat agtatggtaa ttggtgggtc tcgccttaag
     1261 agattagagt gggtaagatt atcttatcaa ttagagtggt aacgcgggtg tatattcgtc
     1321 tcttggtctt tgaccaagag gcttttttaa ttttaagtat acatttgtag atacttttta
     1381 atttgagttt taaagactat tataattttg ccaataagag tggtaccgcg gaaaatcgcc
     1441 tcttagtatt tataatactg agaggttttt tgttttaatt ggaatatttt aatctgtgta
     1501 ttatcaaatg attatcatat aataaaaata aaattctgag actatttaaa tataaaaatg
     1561 gaggtaatca aatgagtaaa ataaaagttg gtattattgg tgcaactgga tatgttgggg
     1621 cagaactgat tagattatta ataaatcatg ataaagccga attgacagca ataggttcaa
     1681 attcgtatgt tggaaaagat atagttgata tatatccaag tataggatat aaaaataaca
     1741 tgatatgtat agaaaatgaa aaagttatag atgtgtgtga tgttgtgttt acagctcttc
     1801 cacatggagt tagtgaaaag tttgtaataa aagcaattaa atcgaaaaag aaggttatag
     1861 atttaggtgc tgactttaga ataaaagatg aagaagtgta tagtaaatgg tatggagtat
     1921 cctttataga taaaatctta cataaaaaag ctgtatatgg attaagtgaa atatacaaag
     1981 atgatataaa agatgcagat ataattgcaa atcctggatg ttatccaact agtatttctt
     2041 taccattaat gccattgcta agttctaaat tgataaaaag caataatata ataattgatt
     2101 ctaaatcagg tcttacaggt gctggtagag aattatcgat tagtagtcac tttacggagg
     2161 ttaatgaaaa tatcactgca tataaaatag ggaaacacag acatactcca gaaatagaac
     2221 aaaatttaag tgaatcctgc caagaaaaag taagtgtagt ttttacacca aatttaatac
     2281 cagtaaatag gggaatatta tcaactatat attgcacaaa agcagataat atttctataa
     2341 atgatataca tagaaaatta actgactatt atgaatttaa agaatttata gaagttctcc
     2401 cattagataa agtagcttct ttaaagaatg ttaggttctc aaataaatgt gtaatatcat
     2461 tacatgaaaa tgtagataca ttaataatct gttcagccat agataatatg attaagggag
     2521 cagcaggtca agctatacaa aacatgaata taatgtttgg tattgaggaa aatacaggtt
     2581 taaaaaatat tgtaccatca ttttagaaat aaaaagtact aaactcatat aattaggagg
     2641 ataaatatgg agattataaa gggtggagtt acagtttcag aaggattttt tgcttcaggg
     2701 attcactgtg gcttaagaaa aaataaagaa aaaagagatt tagctttggt ttattcagat
     2761 gttttatgtg atgcagcagc agtagttact caaaataaag taaagggaaa tcctgtttat
     2821 gttacacagg agcacattaa aaatggaaaa gcacaagcta tcatagtaaa tagtgctaat
     2881 gctaatacct ttaatggaaa agaaggactt ataaatgctt ataaaatggc caaatttacc
     2941 agtgataaat taaagattaa agaaagtgat gttttggttg catctacagg tgttatagga
     3001 aagccattaa acatagattt aatagaagaa aatatggatg aattagtgaa taatctaagt
     3061 aaacaaggtc atattggagc aagagaagct attatgacaa ctgatattat aaaaaaagaa
     3121 atagcagtag caataatgta tggagataaa aaaataacta ttggaggtat ggcaaaaggt
     3181 tctggtatga tacatcctaa tatggcaaca acgttaggat ttataactac agatgcaaat
     3241 attgatggag ttttactaaa agaagctttg aaaattgctg ttgataaaag ttttaataga
     3301 gttagtgtag atggagatac aagtactaat gatatggtaa tgattctagc aaatggaaaa
     3361 gcaaaaaatg atagaataaa caaaaaagat gaacattatc aagtattttt gagtgcactt
     3421 acatatgttt gtatagaact tgctaaactt gttgcaaaag atggagaagg atgcacaaaa
     3481 ctaattgaat gttatataaa tggagcatta agtgaagaac atgcagtaaa attagcaaaa
     3541 acagttatat cttcttcatt agtaaagaca gctgtatttg gagctgatgc aaattggggt
     3601 agaatattgt gtgctttagg ttatgcaggt gaagaaatag atatggaaaa agttgatatt
     3661 atttttgaga gtatgaaagg atatattgaa gtatgtaaaa atggaaatgg actagatttt
     3721 aatgaggaaa aagcaaaaaa aattcttgaa gatgacgaaa taagtatatt agtagattta
     3781 aatatgggca atgccagagg aaatgcatgg ggatgtgatt taagctatga ctatgtaaga
     3841 ataaatggaa gttatagaaa ttaggagggt atactttaat gattaatata gaaaaagcaa
     3901 atacacttat agaagcttta ccatatatag aaaaacatca gggaaagact attgtagtta
     3961 agtatggtgg aagtgcaatg aaaaaagatg gtctaaagga atctgttatg gaagaccttg
     4021 tgttaatgag ttatgttgga atcaatatag ttttagtaca tggtggaggg gcagaaatca
     4081 ataagatgtt agcaaaagtg gatatagaaa gtaaatttgt aaatggtctt agatatacag
     4141 atgaagagac aatggaaatt gtaaaaatgg tacttgctgg aaaggttaat aaagatctag
     4201 taaataaaat acatacaaaa ggtggaaaag cagtagggct ttgtggaata gataataata
     4261 tgattttatg tgacccatat aaaaactatg aacttggatt tgtaggagaa atcaaaaaag
     4321 taaatgttga gcttatagaa tcttgtctaa agtcaggtta tatttcagta attgcaacta
     4381 taggtgttgg agatgatgga gaaacttata atataaatgg tgacacagca gcttctgcta
     4441 tagctaaaga gttaaatgca gataaattga tacttcttac agatgtacct ggcttgttaa
     4501 gagagccaga tgaagaaaaa tcattaataa cagaagttat attagaggat gtcgataaat
     4561 tatttaaaga aggtataatt actggaggta tgatacctaa aatacaggga tgtgtagatg
     4621 ccttaaataa cggagtaaat agggtacata ttttggatgg aagagttccc cattctataa
     4681 taacagagct atttactgat agtggaatag gtacattaat aagaaaagaa aatgaataaa
     4741 attataagtt taagaaggga gaataagaat gaataataac agtacaattt caaaatggac
     4801 tgaatatttt atagatacat ataaccaacc taattttgtt atagattatg gggaaggaag
     4861 ttgttttttt gatactaatg gcaataaata tatagatttt acaagtggat atggagtatc
     4921 ttctcttggg tatagtaaca gtaatttaaa gaatgctcta aaagagcaag tagataaatt
     4981 attacatact tcaaatttat attttaatga gccagtgctg tatgcagggg agaaaataat
     5041 taatgcaagt ggaatggata aaatatattt ttgtaattct ggaacagaag caaatgaaac
     5101 agcattcaaa atagcaagaa agtatagtag tgataaatat ggtaatggta gaggaactat
     5161 tataagttta aaagactctt ttcatggtag aacgatgatg tctttaatgg caactggtat
     5221 ggataaatac cataagtact tttatccact tcccgaaggt tttaaatata tagaaagaaa
     5281 taatattgaa gatttaaaaa ataatcttga ttcgacagtt tgtgcaataa ttcttgaagc
     5341 gattcaaggt gaaggaggag ttaatgttct tgaaaaagat tatgttttgg aactagtaaa
     5401 aatatgtcaa gaaaaggata ttgtagtgat ttttgatgaa gtacaatgtg gtataggaag
     5461 aactggcaaa ctttttggat atgaatattt tgatgtaaaa ccagatatag ttactgtagc
     5521 taaagggctg ggagcaggga taccagtagg aggagtattg gtaaataaaa aactttcaaa
     5581 agtactagga aaaggagacc aaggtacaac ttttggaggt aatctattag ctatggtggc
     5641 agcttcagtt gtattagatg agatatctaa ggatggtttt tataatgaag tattagaaaa
     5701 aggaaattat ataagaaaat ctattgaaag ttttaataat aaagttgtat taaagataaa
     5761 aggtattggt ctaatgatag gaattgaaac taatattgaa tctagtatta tagaagagaa
     5821 agctagaaaa aaaggattgc ttattttgac tgctggaaaa aatgtattga gatttttacc
     5881 accattaaca atcagttatg aagaaataga tgaggctctt gaaatcctaa aagatatatt
     5941 actagaaatt aactaaatat tcaaaaaata gtttgattaa agataaaaat tagaagtaaa
     6001 taaaaaagta aagtgctaac aagtttaatt gaatttaaaa agtttataaa aaggggcgaa
     6061 ttaatatgaa taatataagt ttaaaaggga agagtttttt gacattaaag gattttacaa
     6121 aagaagagat tagatattta ttaactttat caagttattt aaaaactaag aaaaaagttg
     6181 gtataaaagg cgatttgcta caaggaaaaa atatagcttt attatttgag aaaacatcaa
     6241 caagaactag atgttcattt gaagtagcag ctcatgatga aggagcacat gtaacttatc
     6301 taggtccaaa tgattgtcat atgggtaaaa aagagtctgt agcagataca gcaaaagttc
     6361 taggaagatt ctatgatggg atagaattta gaggctttaa acaagaaaca gttgaaagcc
     6421 tagcaaaata ttctggtgta cctgtttgga atggacttac ggatgaatat catccaacac
     6481 aaatacttgc agacttttta acagtaattg aaaatgtaga taaagatttg aataaagtta
     6541 aatttgtata tgttggtgat gctagaaata atatgggaaa ctcactgatg ataggatgtg
     6601 ctaaaatggg aatccatttc gtagcactag cacctaagga gctttggcca aatgaagagt
     6661 tggttaagga aatgaaggaa atagccaaga aatctaaggg aggcataatg ttgactgaaa
     6721 atattgctga tgttaaaggt gcagatgtta tatatacaga tgtatgggtt tctatgggag
     6781 aagaggagca atatgaaaca agaataaatc aacttaagta ttatcaagta aatatggata
     6841 tgataaagaa aactgaaaat gaaaatgtta tatttttaca ctgtcttcca gcatttcatg
     6901 atttagaaac taaggttgct caggagattt atgaaaagtt tggattatca gaattagagg
     6961 ttacagacga ggtatttaat tcgaaatatt caaaggtatt tgaagaagct gaaaatagaa
     7021 tgcatacaat aaaagctgtt atggtttcta ctatgactga tgtaaatatg aacaataatt
     7081 taatatagat attccttaat ttagattttg ttttaataaa aatttataaa atattttaaa
     7141 tatattattc aaaaactatc attatatgtt aaaatagctt atgtatcaac aaattaagag
     7201 ggaggatttg agatatgaaa aaaatcatat tagctttaac aactttttta ttagcaatat
     7261 ctttaacagc atgttcaagt gcaaaaccag aagatacaat caatagtttt tttaacagtg
     7321 caaagaagtt tgactttgaa ggcatgaata aggtaatgga aaataatgat gaaaaatata
     7381 atgatatatt aaaggaatta gatacgaaag acccaaatga acaatatgtt ttagattatc
     7441 taaaacagaa tgcgtctaaa atcacatata caataaaaga cagtgaagtt aaaggtgata
     7501 aggctacaat caaagtagaa tgtaaattta tagattcaac accgttgctt caagaaatag
     7561 tagctgaggc atttacaaag atgcttggta tgagtttttc aggacaagac ttaactgatg
     7621 agaaaacaat tgaaatgtta gtatcaatta tgaaagaaaa acaaaaaagt gttaaagaaa
     7681 cttatgtgac aaaaactgta gagtttgaat gttctaaaaa ggataataaa tggataataa
     7741 gttcagtaaa tgatgcagta gctgatgtat tgttatctaa tcttgttaca gcaggacaag
     7801 aattttctaa ctctataaat gcacttgaat aatattgttt aggtgtagta aagtaataaa
     7861 catatattta tttatgaagg ataccatttt tggtatcctt tttattaata taatgaaata
     7921 catggttaat ttctatattg tcaagaaact atgatatatg ttataagtct atactattta
     7981 agataaactt ataacatata ttttttataa tatagtgcat gaaatttatt taactaattt
     8041 tagatattta ttgacaactg attcagccaa gcattttgat gaatcaacaa tatttattga
     8101 aggatatgat ttttctaaaa ctacatttaa atcagtacaa gcaattacta tattttctat
     8161 agattcattt ttaacatctt cgataagttt attccaaata ttaatattgt gtggattttc
     8221 tttatctatt tttatatttt gaataagatt gtttaatttt atttgccatt catctttaaa
     8281 tataaattca tgttcattat gtattatacc attctggtaa attgtagact caaaagttga
     8341 actagtcgaa aaaagagtta ctttttgtga gagagtaggt aattttttaa ctgtttcttc
     8401 tacaatattt aatagtggta tattaataga cttttttaat tctttaaagt atatatgtgc
     8461 tgaattgcat ggcattgcaa taaaatttac accagttgac tcaagttttt gtaatccatc
     8521 aattatagtt tctttcataa gttcatgatt tattggacgg tcaatataaa atggggtagg
     8581 tattgaatat atcatcatct ttggaaattc atcatcatat tttgctccat attgagattg
     8641 acactcatca ataattaaat caacaaaagg ggctgttgac cttggaccca ttccagccaa
     8701 aataccaatt aatttttcat tcataaaaag tatctccttt atacataaat atatttatgt
     8761 ataaaataaa aatttataaa aacataaa
//
LOCUS       DAMJVM010000126         8712 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.131, whole genome shotgun sequence.
ACCESSION   DAMJVM010000126 DAMJVM010000000
VERSION     DAMJVM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.131"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            119..580
                     /locus_tag="PC523_003176"
     CDS             119..580
                     /locus_tag="PC523_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476667.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILDEKIVNETNFKISYRNYIETLINIQNENLSNFKKKSTKTAKCFNISQIT
                     QLKNLLNNTGYNLLIPYKYLTSRDIEGIVTLINTGSIELGNERIYNYISKA"
     gene            complement(673..843)
                     /locus_tag="PC523_003177"
     CDS             complement(673..843)
                     /locus_tag="PC523_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476668.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(1032..1253)
                     /locus_tag="PC523_003178"
     CDS             complement(1032..1253)
                     /locus_tag="PC523_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HDF2476669.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            complement(1337..2563)
                     /locus_tag="PC523_003179"
     CDS             complement(1337..2563)
                     /locus_tag="PC523_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2476670.1"
                     /translation="MKKVVIIGGGPAGMIAASTACERGCDVTLVEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNEDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNNKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIMDTKPSLIGIEVQESFTRDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTIILDLKPALDEEKL
                     DKRIQKDFQKYTNKKFEKALDDLLPKKLIPVIVNLSEINPDTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIDEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            complement(2624..3556)
                     /locus_tag="PC523_003180"
     CDS             complement(2624..3556)
                     /locus_tag="PC523_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDF2476671.1"
                     /translation="MDIQLSILLFFQNLRNPILNFIFLVLTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYIHVIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVIF
                     IKIFDYIDDNKNYILLLVLLIPFIIVAIFLNSSEYFEKFGIIVGFVFGYIVEDRFVKF
                     NTDNNNKKINFSSKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSINFMNFIKNTVVVFYGVAGAPALFKVLKLN"
     gene            complement(3645..4172)
                     /gene="hpt"
                     /locus_tag="PC523_003181"
     CDS             complement(3645..4172)
                     /gene="hpt"
                     /locus_tag="PC523_003181"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDF2476672.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEEIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            4401..5477
                     /locus_tag="PC523_003182"
     CDS             4401..5477
                     /locus_tag="PC523_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2476673.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSMDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSMDKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTNTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNIDLWNINIIASKMGEIMFSDLDFISNTISLVSAERNY
                     LLKELKNIKNLNVYDTKGNFILCKIKTKELTAKSLREKLIPQKIIIRDCCSFEGLNEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            complement(5585..6793)
                     /locus_tag="PC523_003183"
     CDS             complement(5585..6793)
                     /locus_tag="PC523_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HDF2476674.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFVVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGSKEKQSA"
     gene            7192..7374
                     /locus_tag="PC523_003184"
     CDS             7192..7374
                     /locus_tag="PC523_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476675.1"
                     /translation="MINIKPKENKHMTKTMICDYCNKVINNNSEYITFHKKNYVKTSV
                     CINCVLKLMDKTKENK"
     gene            complement(7696..7884)
                     /locus_tag="PC523_003185"
     CDS             complement(7696..7884)
                     /locus_tag="PC523_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2476676.1"
                     /translation="MIKMRLKGKVTVLILACLVSIGVVGCGDKENNDTTKNKAEKSIT
                     DGQENSTDTAPDVTSDSK"
     gene            8287..8604
                     /locus_tag="PC523_003186"
     CDS             8287..8604
                     /locus_tag="PC523_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476677.1"
                     /translation="MNFGNINLIIIGVGIIILTTIISLIKPKISFCSEKYFNKLESIY
                     GNIDRKRTVKLEVLSRYVMGLEYIVIGLFTRRLDITIIAMIIVAVITTVLYYLIRKKY
                     ITI"
BASE COUNT         3061 a         1339 c          953 g         3359 t
ORIGIN      
        1 taataaaaaa taacaaatat tttatgattt agtatttatt gtaaataaat tattgaagct
       61 ttattagtta agtatagatt tttaataact atttttataa ttagaaggaa gtggttttgt
      121 gcaaatagaa caacttgatt tagaaacgag aaataaaata tactgctata caaaaaaaat
      181 tcttagaaag tatcaaaaag gtataacatc tggaaaatta acagccgata aatttgcaga
      241 taatatattg tctcaagatt ttatcagtag tatacttgat gaaaaaatag tcaatgagac
      301 caactttaag atatcttata gaaactatat agaaactttg ataaatattc aaaatgaaaa
      361 tttatctaac tttaaaaaaa aatctactaa aactgcaaag tgcttcaata tctctcaaat
      421 aacccaatta aaaaacttac taaacaatac tggttataat ttattaattc catataaata
      481 cttaacttct cgtgatatag aaggaatcgt tactcttatt aatactgggt ctattgaact
      541 tggaaatgag agaatctata attatatatc caaagcataa taatttaaac ctattataaa
      601 aaggcaattt atttaataaa aaatattttt aatattaaat aaattgcctt tttaattttg
      661 gactaaatac tgctatccaa gtgctttttc tagtttagat aaatgttgta ttggataata
      721 ttttagtagc tctttaaact gttctactga tagaccttct gttgttgtat atattcttat
      781 tttttcatct aagtcactat taataaattt gttttttaaa tcctcaaaat ttacactact
      841 cattttacat cactccttat gtaacatatt catttgtatt attttatcca aatacataaa
      901 atttattaca taaaaatata tctatttaat aatttaataa aaaaaagcta tctcaattta
      961 gaaattactt tctatatttg agatagcttt agctaaacta atagccaata gttataaata
     1021 attatttata actatctgtt gctttttcca gccatttgtt gttcagccat ttcaactaat
     1081 tttttagtca tgtatccacc tacataacca ttttctcttg cagtaaggtt tcctttatca
     1141 acttgttgat aattactcat accaagttca ttagctattt ctaatttcat ttggtttaaa
     1201 gcctcttttg ctcctggtac tactgttctg ttgttgttat tgttacttgc catgttgatt
     1261 accttccttc tgtttaattt attttgtgtt gctgataata ttatgtgatt ttaaacattt
     1321 ttaattcagg taatttttag caattaaaac ctgctaaata tccagtagaa aaagctattt
     1381 gcaaattgaa tccacctgta tatgcatcta tatctattac ttctccagca aaaaataaac
     1441 cttctactaa ttttgattcc attgtacttg aatttatttc atttactttt acaccaccag
     1501 aagttattat agcttcatca ataggtctgt atctctttac tgtaaacttt aaatttttta
     1561 ataaatggac taagtttttt ctctgctctc tagaaatttg gtgaacaact gtatctggat
     1621 taatttcact caaatttact attacaggta ttagtttttt aggaagtaaa tcatctaatg
     1681 ctttttcaaa ttttttattt gtatacttct gaaaatcttt ttgaattctt ttatccagtt
     1741 tttcctcatc taatgcaggt tttaaatcta atattatagt gtaattttct ttccttgtat
     1801 ctttcattct acaacttgca gacttgatta taggtccatc taaaccaaat ttagtaaatt
     1861 caagttctcc aaaatcacta taaactttct tttgtttgct attaaatact cttatttcaa
     1921 catttctcaa tgataatttt tctaaatctc ttgtaaaact ttcttgtact tctataccaa
     1981 ttaaagatgg ttttgtatcc ataattgtat gtccttgtga aattgcaaat ttatatccat
     2041 caccagtaga accagtaagt ggataactta atccaccagt tgctactaca actgaatcac
     2101 atttaatttc tttcttattg tttaaaatta ctttttcaat tttattattc ttagatatta
     2161 ttttttctac tttcgaattt aataatatat ttactttctt acttttaagt tgtctttcaa
     2221 gagcgtttac aatatcaaat gctttatcgc tctctggaaa aacccttttt cctctttctg
     2281 ttttagtttt tactccaaga ttattaaaca tacttattac atcttcattt gtaaaagtat
     2341 aaaaagcact ataaagaaat tttccatttg taggtacatt ttctattagt tcttctattt
     2401 cacaggcgtt ggtaatatta catctacctt ttcctgttat tgcaagtttt tttcctaatt
     2461 tatgattttt ctcaactaac gttacatcac agcctctctc acatgcagtt gatgcggcta
     2521 tcattcctgc tgggccacca cctattatta ctaccttctt caaataatca ctcttttcta
     2581 cccttaattt ttttaattta aactattgat tatatacctc aaatcaattt aattttaaaa
     2641 ctttaaataa tgctggagct cctgctactc cataaaaaac aactactgta ttcttaataa
     2701 aattcataaa atttatactc atagtagagt cacttatatt taaagtaact attaacattg
     2761 aaataaacaa cttaatgcca atatataaca ttcctattgt tactatccca accaaaaacc
     2821 tacatattct tgaaaaaatt atatttttac tccccttatt tttactgcta aaatttattt
     2881 ttttgttatt attatctgta ttaaatttaa caaatctatc ctcaactata tatccaaata
     2941 caaaccctac tataatacca aatttttcaa aatattcaga gctatttaga aatatagcta
     3001 ctattataaa cggtataagt aaaactaaaa gtaaaatata atttttgtta tcatcaatat
     3061 agtcaaatat ttttataaat attactgtaa aaaatatacc taatatccaa ccagctatca
     3121 catctattgg ccagtgaaca gctaaataca acctagacaa ccctactcct aaaaccatca
     3181 ctgcacctat tacatggata tatctctttt taaatatagt cataatacta acccaaaacg
     3241 tggttgcagt ttgagtatga gcacttggaa aagaataacc gccagcagta gaaactctca
     3301 atgactcaag accttttatt cctattggtc taggagcttt tacaaactct tttatgccta
     3361 aatttacaac aaaatttcca attatagcaa acagtatttt ctgtccacat tttttattta
     3421 tacaccagta tataattgtt gtaattaata ttaataacgg tgcttctgtg cttatcgtaa
     3481 gtacaagaaa tataaagttc aaaattggat ttcttaaatt ttggaaaaat aataatatac
     3541 ttaattgaat atccatatag atgtctcctt tactaaaatt ttatataatt aactaaaaag
     3601 cactaactcc cttagaaatt agtgcttttg attcacattt aaatctattc tacgtcctca
     3661 actataccta tgtaaggtaa atttctatac ttttcagcat aatctattcc ataaccaaca
     3721 ataaatttgt cttctataac aaatccaaca taatctacag gtatattagc ttttcttctt
     3781 tgaggtttgt caagtagagt acacagtttt aaagattttg gatttctagt ttttaaagct
     3841 gccactagat tacttaatgt taaaccagaa tctattattt cctctacaat caacacattc
     3901 tttccttcaa tatctacatc taaatctttt aatattttaa ctgttccaga agactctgtt
     3961 gaattgccat aacttgtaac actcataaaa tctatattta catccaaatc tatacatctt
     4021 attaaatctg atacaaatac actagctcct ttaagtatac ctaccactag taaatcttcg
     4081 cctttgaagt cttcttctat cttctttcct agttcataaa ctttctcttt tatttgttct
     4141 tctgtcaaca tttttcctgt aactttgtac attttgtatc ctcccgcttt ccttctatta
     4201 acttatatat tatagcatac caaaatatca attaaaatct ttttcagcat ttttaaacct
     4261 aaattaacag tttatccata cttttttgct tttttcatta aaaatttgga ataacgattc
     4321 ctttgtatta taatctatat gtacatatat taatcagtta tttttctcga ttatataatt
     4381 ttatttttgg aggtactttt atgagcacaa atcatggagc taatttatat agtttatcta
     4441 gtaagtatgg cttttcaaaa gaagaattta tggactttag ctcaaatata aatccttttg
     4501 gtacatcaag tttagccaaa caatacattg taaacaatat agatatggta tctatgtatc
     4561 ctgaccctga ttacatagac ttaaaaactt ctatatccaa ttattgcaaa tgttctatgg
     4621 ataatatagt acttggcagt ggagctacag aactaatttc atcatttata catacaatta
     4681 atcctaaaca agctctctta ctctccccag cttattcaga gtatgaaaaa gagttatcta
     4741 aaattaattg ttctatggac aaatacttcg ccaaagaaga agataatttt catataaatt
     4801 tagaaaatct tataaaaact atcaatgcta aagattatga tttggtagtg atttgtaatc
     4861 ctagtaatcc aactggattt gcatttacta aagtagaagt gagagaaatt ttaaaaaata
     4921 cagactcatt tttaatgatt gatgagactt atgtagagtt tactgataca aatacatact
     4981 cttgcactca attagttgat gattattcta atttattcgt tataagaggt acatcaaaat
     5041 ttttttcaac gcctggcata aggcttggat atggtcttat atctaacact aatgttaaaa
     5101 atgaaataaa taaaaatata gatttatgga atataaacat aatagcttct aaaatgggag
     5161 aaatcatgtt tagtgattta gattttatat caaatactat ttctttagta agtgctgaaa
     5221 gaaactattt acttaaagag cttaaaaata taaaaaattt aaatgtgtat gatactaaag
     5281 gtaatttcat attgtgtaaa ataaagacaa aggaattgac tgctaaatct cttcgtgaga
     5341 aactaattcc tcaaaaaata ataataagag attgttgttc atttgaaggt ctaaatgaat
     5401 atttctttag ggtatgtata ttaaaaccta atgaaaataa attattaata tcatctctta
     5461 aagcaatttt taaataaaaa attactttat cttataattt gtacaatcat acttttatca
     5521 tttcaacaat gtaaaagagg cttatctcta ttttgagata gcctcatttt tatctattta
     5581 caaactaagc actttgcttt tctttgcttc cttttttact cattccatca tttattttat
     5641 ctactactac tgctattgca ttatctcctg atacattaca tgctgtacca aaactatctt
     5701 gtgttaagta taaagctatc attaaacttg caagtccacc atctgatggt attccaacca
     5761 ttggtagaaa aggaagagca ctcattatag ctccacctgg cgctcctggt gctgcaacca
     5821 ttgctatacc taacattgca atataaggga ataatacacc tattccatgt ggcataccgt
     5881 tcatcattaa aactgctgtt gtaaatcctg ttattgatat catactacct gataaatgta
     5941 ttgttgcaca aagaggtaca acaaattctc ttatttgctt acttactcca tttttttctg
     6001 cacaaccaac attaactggt atagttgctg ctgaagattg agtccctata gctgttaaat
     6061 atccaggtac ttgatttttt aacattctta ctggattttt tcctgaaaat acgcctgctg
     6121 ctgtgaattg acatgccata tatacaaaat gtaaagggat tactactaaa tagactttcc
     6181 aaaatactcc taatattgtc catacttctc ctgctatagt tatatttgca aaagttcctg
     6241 ctatgtataa aggtaataaa ggtataatta ctgttgacaa cgttttcgtt acaattcctt
     6301 gaaattcttg gaagaaatta aataaagtct ctccttttcc gtgatttctt aaagctgata
     6361 ttccaagtcc cattacaaat gcaaatacga ttgcagctgt tacatctacc ataggctcta
     6421 atggaattgt aaatattgga gctaacattc ctgcctctgg gtctccaatt tgacttgcaa
     6481 cactcgcatc aataaaattt gggaagatta atgtagatac tacaaatgat aataatcctg
     6541 ctattaaagt agaaccatat gctattcccg tagttatccc caataacttt ccggctcctg
     6601 ttgctaaatc agctatacct gctgttacaa aacctataat tataaatggt ataacaaatt
     6661 ttaggaatgc tgagaataaa gtactaactg ttactaatat ttgtacgatt atttctggtg
     6721 cataacttcc gattaaaata ccgactatga taccgatgat tagtttgggc actaaaccta
     6781 tttttttctt cataatattt accctccata acttttttaa attaagcatt cttgtacatt
     6841 gaatacttag ttaaatattg gatatccact tattatgata atagagtttt acctctccgt
     6901 caacaattta accatttttt gttaataaaa aaaagaataa catatataaa gtgttccttt
     6961 tatttatttt attttatata tatgtctatt ttcaatattt tataatgttg atactcttta
     7021 tttttttaac tttcgtcagt tgaatgtcag taaaatattt tatccacata tttcatatag
     7081 ttttcccaca acttatcccc gatattattt ataaacaaaa agatagatac tagtaactct
     7141 gttttaacta gtatctatta aggtatacac caaatatatt agaacttaat tatgataaat
     7201 ataaaaccaa aggagaataa acatatgact aaaactatga tatgtgatta ttgcaataaa
     7261 gtaataaata ataatagtga atacattact tttcataaga aaaactatgt aaaaactagt
     7321 gtatgtatta attgtgtgtt aaaattaatg gacaaaacta aagaaaataa gtaataaaca
     7381 atattatttg agaaagtgaa gaaaacactc taccataagg aaaatgctct atctaatata
     7441 aatatggtaa aaaagtaaag atgtattggg cgtacatctt tactttttta ccatatcaca
     7501 ttactaccct aataaaaaca gctttatgta caaaaacacc atttaaaaag gatgttttct
     7561 tggataatat tgttttaact agtaaagata tattgagggg ttatctttac cttttttatt
     7621 atattataaa tttcctaaaa gtaaaattat aattaaaata tgtataaatt acaataatta
     7681 gtaaactatc ttttattatt tagaatcact tgttacatct ggtgctgtat cagttgaatt
     7741 ttcctgtcca tcagtaatac ttttttctgc tttattttta gttgtatcat tgttttcttt
     7801 atcaccacaa cctacaaccc caatagatac taaacaagca agtattagga cagtaacttt
     7861 cccttttaat ctcatcttta tcatcctcct agataaatat taattcacta tttaaaatta
     7921 tagcaaagta atgttaatct tatgtttatt gtaaaaaata tgtaatttat atgcttagaa
     7981 tattatattt tctaacaaaa tgtaatatag tagaaaatac attaatgaat ttcaaaaaat
     8041 aagcactcat ataaagagta ctttagtgta tattaatgaa tgtttataat ctaatttaac
     8101 atgtcttatg ttttagtatg ataattttcg ttcgctttat tattacaact tatgttctag
     8161 ttttatgtat cctgctaata tttgtaatat tttagatacg ctttatgctt atgttgctga
     8221 tatactttga ttaagaaaaa aaatctaata taaactggtg attatattat aaaggaggta
     8281 tctactatga attttggaaa tattaatctt ataattattg gagtaggaat tataatatta
     8341 actacaataa tatctttaat taaacctaaa attagctttt gtagtgaaaa atattttaat
     8401 aaattagaat caatatatgg aaatatagat agaaaaagaa ctgttaaact ggaagtgctt
     8461 tctcgatatg taatgggtct tgaatatata gtaataggtt tattcacaag aagattagat
     8521 attacaataa tagcaatgat aatagtagcc gttataacaa cagtattata ttatctaatt
     8581 agaaagaaat atataactat ataaaatcta ataaaattaa tactagaaga aggtagcaac
     8641 tagttacggt tgctaccttc taatctattt tactaaattt tctatttcac taaatgcttt
     8701 ttcatttcca tt
//
LOCUS       DAMJVM010000127         8636 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.132, whole genome shotgun sequence.
ACCESSION   DAMJVM010000127 DAMJVM010000000
VERSION     DAMJVM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8636
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.132"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     misc_binding    116..327
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            490..1779
                     /locus_tag="PC523_003187"
     CDS             490..1779
                     /locus_tag="PC523_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDF2476678.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGIPVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIVATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSMKNNQEIDKAV"
     gene            1833..2912
                     /locus_tag="PC523_003188"
     CDS             1833..2912
                     /locus_tag="PC523_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HDF2476679.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     SKSDSKGAVENILKELSGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2933..4477
                     /locus_tag="PC523_003189"
     CDS             2933..4477
                     /locus_tag="PC523_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDF2476680.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKDKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGNTLTLSDIVTKLDMAFEDRKSNVENLDKEYCDEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            4592..4783
                     /locus_tag="PC523_003190"
                     /pseudo
     CDS             4592..4783
                     /locus_tag="PC523_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5036..5578)
                     /locus_tag="PC523_003191"
     CDS             complement(5036..5578)
                     /locus_tag="PC523_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HDF2476681.1"
                     /translation="MKRYIALLRGINISGKNKIVMSELKAGFVELGYSAVSTYLNSGN
                     VVFLSNVDDSEVISNSIGLMIKKQFELEIPVFIISQEVLEDILNNAPSWWGDNNKEIY
                     DNLIFMFPDLSYDKFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYQKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(6027..6311)
                     /locus_tag="PC523_003192"
     CDS             complement(6027..6311)
                     /locus_tag="PC523_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2476682.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYVL
                     VYRENEDKEKNEHDKNLNETINKFKREEEKRIQAKSAARAIAEAKKRVLY"
     gene            complement(6372..7136)
                     /locus_tag="PC523_003193"
     CDS             complement(6372..7136)
                     /locus_tag="PC523_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDF2476683.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAEASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMIDTIENFRKSNKTFKEF"
     gene            complement(7418..7684)
                     /locus_tag="PC523_003194"
     CDS             complement(7418..7684)
                     /locus_tag="PC523_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2476684.1"
                     /translation="MIESRCGILCYECEYKEKVNCKGCTDIKKPFWGEQCPVKSCCEN
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRIVQCKKWAVLAL"
     gene            complement(7726..8535)
                     /locus_tag="PC523_003195"
     CDS             complement(7726..8535)
                     /locus_tag="PC523_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2476685.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKENL
                     TLVPDASGCMIFKFDKKGIESSFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKANIVDMT
                     IPILENARKQNSIMSVKSISQISCYSERHLNRIFNNSLGMSLKSYLRLLRINLVLQEI
                     QNNKIPFVTLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
BASE COUNT         3041 a         1106 c         1377 g         3112 t
ORIGIN      
        1 atagtatgaa aatttacata taaaactaaa aaatgcaagt aattgacttt ttaagttgac
       61 aaaatattgc atatatatta tgatataaat cataaattaa ctacaaatca atattatata
      121 ttcgtagaag aagaaagtag cataaaggaa ttttacagag agttagattt ggctgagaac
      181 taatattgga atttatgcga aaaggacttt ggagaatact attgtaacta tgttataata
      241 gtggtttaat tcgccttaag aaaatgagtg agtaaaatta atttttacta ataagagtgg
      301 taacacggat ttatattcgt ctcttggatt tttccaagag gcgtttttta ttataaactt
      361 atttcagaaa atttaaaact atatattaaa tgaaaagtat aaaaatatga tagttaataa
      421 atttataaat tagaaaattg taaaatatac taaagttagt atctttacga tttaaatgga
      481 gggatgaaaa tggaagaaga aaaaattaac tatgctctgg aacagattcc agagaatcaa
      541 aaacgtggtt gggtagctat gttttcagtt ttagtagcaa taggtgtgga tttatcatct
      601 gttattttag gagctgagtt ggcacagagt atgccaatga aacaagcaat attgtcagta
      661 attgttggtt catttttttc tgcaattttg tatactacat gctctttggt tggttcatct
      721 acaagtctat ctacatctat gataacaaag tatgtatttg gagaagctgg agcaaaaata
      781 ttttctctag ttataggagt ttctctatta ggatggtttg gagttcaagt aggtttcttt
      841 gctcaaaatg cacagataat aataaaagat atatttaatt tagatgtgag tatgcagatt
      901 ttaagcctaa taggtggtct cttgatgatg agtactgcaa tttatggata taaggcaatg
      961 gaaaagttga gtgtgtattc agtaccgttt ttattagttc ttatgatgtt aactatattc
     1021 ttagctttta gagcaaatgg aattccagtt gatgataata tgaaatctac tatgacattt
     1081 gcaggtggag tttctctgtc aatgagtata ataatcgttg gtgcaattgt atctcctgat
     1141 ataagtcgtt gggctaaaag tagaagagac tgtgcgcttt caagcttttt aggaatacaa
     1201 tttggaaatg cttttatgat aatagtttcg atagtattag taaaatgtat gggaacttca
     1261 gatattatga gaatttttat tacattaggg attgctatac caggtataat agtacttaca
     1321 ttagctcaat ggactacaaa tacaagtaat gtatattcag catcattaag tatagctctt
     1381 gtacttaaga aggctcctga gaaagtatta actatagtcc ttggtattgt agcaacatta
     1441 ttggctgtat ttggaatata tgaaggtttt ataggatttt taaatttact tggaattgta
     1501 attgcacctg ttggtggtgt atatacagca gaatattata tagtaaagca agaacttaaa
     1561 ggctttgata aaggtgtatt atataaacca atagttaaac gttcaatagt gtcttggata
     1621 atcggtatat taataactta cctttcaaca tatggattta taactcttac tactatagca
     1681 cctttagatg gttttatagc agggtttgta gtacaatcta ttattggaaa agtgctttgc
     1741 tcaatgaaaa ataatcaaga aatagacaaa gcagtataaa gaaatttgtt tataaagtat
     1801 aaaataaatt agaaatatga ggagaaagta acatgagatt tttaacagaa gaaagtgtag
     1861 ataaaatagc agttggtgca gccgtgcttg gaactggagg tggaggtgac ccatatgtag
     1921 gtaagttggt tgcaaaacaa gccattaaaa agtatggtcc tgtaaaagta ataagcttag
     1981 atgaacttga tgatgatgct ttggtagttc cagtatctgg tatgggttct cctgtaatta
     2041 caatagaaaa attactttca gaagtagagt taactacacc tttagaaata atggagaaat
     2101 tattaaatag aaaagtggat gttataattc ctatagaaat aggaggaata aactctctta
     2161 tgccgatagc agttgcagct aaaaaaggac ttcctatttt agatgcagat tctatgggta
     2221 gagcttttcc tgaagctcaa atggtgactt tttatcttga aggatatgag gcatctcctg
     2281 ctgtaatgtc tgatgaaaaa ggaaattctg ctattcttta tcctgtagat ggtatttggt
     2341 cagaaagatt agctagaact ttaactgtag aaatgggagg aagttcttct attagtgact
     2401 ataatttaag tggagctcaa gttaaaaaag ctgctatagg taatactctt acaatagctg
     2461 aaactatagg aggattttta cttgaaagta aatctgattc aaaaggtgct gttgagaata
     2521 tactaaaaga attgagtgga tacaaattat ttgaaggaaa agtaattgat attaagagag
     2581 agttgaaagg tggatttact agaggacatg ctttttttgc tgggattaat gaatatgatg
     2641 gagagtattc tatattattc caaaatgaga acttaattgc caaaaaaggt gatacaccac
     2701 tttgtattac accagattta atagcagtgt tagatttaga aacaggattt ccaataacta
     2761 cagagagaat aaaatatggc tctcgtgtta tggtagtagc ttttccttgt aatgaaaagt
     2821 ggagaacaga aaagggaatt gagacagttg gacctggata ctttggatat gatgtggaat
     2881 ataaaacagt agaggaatta caaggaaaat aaaagtttag gaggaaattt tgatgtatcg
     2941 tgtaggaatt gacgttggag gaacaaatac agatgcttgt atactggatg gggaattaaa
     3001 agtaattcat tcagttaagg tggcaactac aaaggatgtt gaaactggtg tttacaatgc
     3061 cttaaaaaaa gttatagatg aaagtaaaat tgaccattca ttaataaaat atgctatgct
     3121 tggtacaact cattgtacaa atgctattgt agaaagaaaa agacttaata atgtggctat
     3181 gataagaata ggaaagccag caactactgc cattcttcct tttatagatt ggccagagga
     3241 cttaagagaa aaaatagagt tagattcaat attggtatct ggtggatatg agtttgatgg
     3301 tagaaagata aatgaacttt gtaaagatga agttgtagaa ttttgcaata gaataaaaga
     3361 caaagtggaa agtgttgcaa taagtggagt atttgctcca gtaaataaac aacaagaaga
     3421 agaagttgca aaatgggtta gagaaattct aggggacatt cccatatctt tatcaaatga
     3481 aataggtaat atcggtatat tagaaagaga aaatggggct attttaaatt ctgcattgtc
     3541 tcaagtaggt aaagctgtat ctttaggatt tgaaaaagca cttaaagatt taggcataga
     3601 cgctcaaata tattttagtc aaaatgatgg aacattaatg aatttagagt atacaatgaa
     3661 atacccaatt tttacaattg gatgtggaat aacaaactct ataagaggag cttccttttt
     3721 atcaaaagta aaaaatgcaa tagtactaga tgttggagga actacaagcg acttgggtgt
     3781 actatacaat ggattcccta gagaatcatc aattccagtt acaattggag gggtacatac
     3841 aaactttaga atgccagatg ttctaactat tggtcttgct ggtgggacat gtgtaaaagg
     3901 cgaaaaggat aatataaaga taggtcctga aagtgtggga tatagaataa ctgaagatgc
     3961 aataatattt ggtggaaaca ctctaacttt atcagatatt gttacaaaat tagatatggc
     4021 atttgaagat agaaaaagca atgttgaaaa cttagataaa gagtattgtg atgaggtata
     4081 taagaaggtt attttaaaat tagaagatgc tgtagaccaa atgaagacta gtcaagatga
     4141 tgttgaattg gttttaactg gcggaggaag tataattgtt cctgaaaaac taaaaggcat
     4201 aagcaatgtt ataagacctc ctcattatac tgctgcaaat gctattggtg cagctttagg
     4261 acaaataagt ggagatgtag aaaaagtata tagtttagat aaaatgtcga gagaagatgc
     4321 catagaagat gcaaaaaaag aggctgtaaa taaagctcta aaagctggtg ctaaagaaga
     4381 tactatagaa gtattaaaca tagaagatgt tccacttgca taccttcctg ggaatgcact
     4441 tcttataaaa gtaaaagttg ttggtgattt aatttaaaga ataaatataa gtaatttcag
     4501 ggtctttaaa ataaagtgtg tattcttcta aaaagtggtg ataattaaaa ctattaaata
     4561 tcactaatac aaaggaaggt gcacactttt tatgtttaac aaaaacgaaa tataaaaaaa
     4621 acttattgaa gaatatgatg taaagacaac tacagatatt caagatatgc taaaagattt
     4681 atttgcaagt acaattcatc aaatgttaga agcagagctt gacaagcatt tagattatga
     4741 cagatacgat aataagaaca aaaatactaa aaactctaga aatggatatg aacagccgat
     4801 atatacctat tttttaaggt ttattggctg tttgcttttg acaggtagac catacaattt
     4861 atacaaattc ttatatttat actctaaatc attatacatt ttattgcaaa gcatataatc
     4921 agacttagca tttctatatt tgcttttaca tctggcatat aagtgattcc atttgtcaac
     4981 taactggatt ttgtactttt tataaaaaac tattttatat agagatttct gtcttttatc
     5041 gcattccaac tatttttctt actgtatttg ctgtacgaat tgtaatctta tcacttacat
     5101 ttgaactagc agtctttgac caccaatttg tcttttgata atctttacga ctaaaagacc
     5161 aaaaaatagc atttttatag tgatacactt tttcatattc ttctttagga tttccaacct
     5221 catcataaaa cttgtcataa gataagtcag gaaacataaa aataagatta tcatatattt
     5281 ctttgttgtt atctccccac caactaggag cattatttag tatatcctct aacacctctt
     5341 gtgaaataat aaaaactggt atctccaatt caaactgttt ttttatcatt aatcctattg
     5401 aatttgagat aacctcgctg tcatctacat tacttaaaaa gacaacattg ccactattaa
     5461 ggtatgtaga aactgctgaa taaccaagtt ctacaaaccc tgcttttaat tcagacataa
     5521 ctattttgtt cttaccactt atattgatac ctcttaataa tgcgatatac cttttcatat
     5581 aataatttca cctctaaaca tttacatttt tctatgtagc acaaactgga atttgtatct
     5641 tttataattt ttttaaagct tttttatatg taatttcccc aagtataaac tgttctaaag
     5701 catttatttt tttcagattc agtattgtat ttttcaacaa ttcttctaat aaatctattt
     5761 cattcaaaat cggtttataa atatgtttac aacaattaaa ttcttcaaag tatcttgaac
     5821 caaatcatat ttgatattct acattgatat ggatttgaaa ataaggtatc tcctgtgata
     5881 aaataacagt ttttatgttt tttaacatat ttttgaagtt ctttattaat caatttacat
     5941 tctgtacatc ctttatcaaa tacaaattta catacttttt ttaatataaa aaatcggtgc
     6001 ctaatttgta ccgatttttg tttttcttag taaagaactc gcttttttgc ttctgctata
     6061 gccctagcag cactttttgc ttgtattctt ttttcttctt ctcttttaaa tttatttatt
     6121 gtttcattta gattcttatc atgttcattt ttctctttat cttcgttttc tctgtatact
     6181 aacacataat ctattattga ttcgtttatt atctgggata tacttttatt ttcattaatt
     6241 cctattctct taacttcctt aagtagagtc tcatcaatat gaaccgttgt ttgctttgta
     6301 ttttttgcca ctttaatcac ctcttaaaaa attctaacat taaaattttt tttggtcaat
     6361 aaattttaat attaaaattc tttaaatgtt ttatttgatt ttctaaaatt ttctattgta
     6421 tctatcatct tattttttat attttcacct aatacagaag actttagtct aggattttcg
     6481 atatggtcat aaattggttc taaaaattcg acttctacag gaacagaatt aattttacca
     6541 ataggttggt atccttcata agtatctttt gaatttttta ttactagtgg aacaataggg
     6601 cattttgctt tatatgctat ctttaaagct ccacttctaa actctgaaac caatgaattt
     6661 ggttctttaa tccatgttaa atctccttct ggaaacactg ccatactttg acctgtaagt
     6721 atattttgag aggcttcggc tatactcttt ataccctcac gattgttttt tctgtttaca
     6781 tatacacatc ttagtaattt tgcccattct ttaaatatag gtatatttct ccaaactggt
     6841 tcatctgcaa ttacacatcc aattggtctt tctacacttg ctgttagtat gaagctatca
     6901 agcatactag aatgatttac aacaaataga agtggttctt ttggcaatgt ttctttacca
     6961 ataatattaa gttctatatt cactatatct aaagattttt tagcttgttt ttgcataaat
     7021 tcaaattttt cttttaagct aaatttatca ggattctttt taattttaat gagcctaggt
     7081 atagaagatg taaaacctat aagttgatat gtagcaaatt ttaagtaatt attcataaga
     7141 ttatctcctt aaaataaaat tatttattac tgtaaagatt ttaacataaa ataaaattta
     7201 agtctatttt aattcaattt tctaacagat atttcaaata cacagagatt aagattttat
     7261 atttcaaatt ttagtaatca gattttatca ataagttact attctattaa tgtaattaaa
     7321 taacctttaa atttaattac aaaaactgtt gcagattttt ctacaacagc ttaaaagact
     7381 tatcttataa aaacttattt tataataaga aagtatatta taaggctagg acagcccatt
     7441 ttttacactg aacaattctt ttaccatcat caccttgttc tttgtcatat gcaaattgat
     7501 taagcatttc acatggaaat gtatcacata gaccacaatg tgtaaggttt ttattttcac
     7561 aacaagattt tacaggacat tgttcacccc aaaatggttt cttaatatct gtacaacctt
     7621 tacaattgac tttttccttg tactcacatt cataacataa aattccacat cttgattcta
     7681 tcattctaag tcactcctta aatataaatt ttatatgatt gtatatcaaa atttatactt
     7741 ttcattgtaa aaatcggaca gattttttat ataggttgtt ggattaacac cacaaataga
     7801 tttaaaatca tttataaaat gggattggtc gtaataacca atatcttgtg ctaaagttac
     7861 aaatggtatt ttattatttt gtatttcttg caacacaagg tttatacgaa gcagtctaag
     7921 gtatgattta agactcatac caagagaatt attaaaaatt ctatttaaat gtcgttcact
     7981 ataacaacta atttgggaaa tgcttttaac agacataata gagttttgtt ttctggcatt
     8041 ttcaagaatt ggtatagtca tatctactat atttgctttc aacaagtatg ataaaaataa
     8101 catatctaat tgttctacta attcttttat agtacttgtt ctttcaaaaa tagaattaac
     8161 ctctaagcta aataatgtgt taaaatcttc aagtgaaatt tttaaatctg tagattctct
     8221 ttgagaaaga cctgtcaaat aatatactcc acctgggcga aattcaacaa atactctaaa
     8281 aagtacattt tctatatcat ttttcacaat agtggttttt gttgtagctc cccagaatga
     8341 actttctata ccttttttat caaatttaaa tatcatacat ccactagcat caggtactaa
     8401 tgtaagattt tctttaatac ttgtgtctgg aatagatatt gtatagtgtg ctatatactg
     8461 tcttaacaat ttatgtggca aaatatatgt atactgtttg caggtattta aaatgttatt
     8521 gattatttta ttcataagta tatcctcctg agatataaaa aataagtata ctaatttact
     8581 ttttatataa taaaccagtt ttgatttaat tgaaatattt taataagata ttttgt
//
LOCUS       DAMJVM010000128         8465 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.133, whole genome shotgun sequence.
ACCESSION   DAMJVM010000128 DAMJVM010000000
VERSION     DAMJVM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8465
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.133"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(128..736)
                     /locus_tag="PC523_003196"
     CDS             complement(128..736)
                     /locus_tag="PC523_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDF2476686.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISN
                     FYYDDKGKKVFSKDKLDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(845..3583)
                     /locus_tag="PC523_003197"
     CDS             complement(845..3583)
                     /locus_tag="PC523_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HDF2476687.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNISLYTVSINMYRIILLW
                     IFGYSIVYISNFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFRDINWNMIIVLINVCISLFNLLY
                     LLNKLDSNIEHYKLIKDLTYFIFFTTIIGFVAIYKINIVDSNRFIGVIYLLLCNHTYY
                     VYVYSLRNRVVYPYYELDSINKKLSKKSEQLGKINLVIEKDMIIQRKLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMDNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNGKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRHINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCTSSDLVDMTLEQYFETFNIDFF
                     DNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLVE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNSIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIEKEKLNDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKIIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3731..4522)
                     /locus_tag="PC523_003198"
     CDS             complement(3731..4522)
                     /locus_tag="PC523_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2476688.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKALDEIKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIQIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4559..5215)
                     /locus_tag="PC523_003199"
     CDS             complement(4559..5215)
                     /locus_tag="PC523_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2476689.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSAINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALVILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5208..6173)
                     /locus_tag="PC523_003200"
     CDS             complement(5208..6173)
                     /locus_tag="PC523_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDF2476690.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGNVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERRTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(6279..6385)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(6551..7129)
                     /locus_tag="PC523_003201"
     CDS             complement(6551..7129)
                     /locus_tag="PC523_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF2476691.1"
                     /translation="MRRKFVFWTPDMHCSTPLLKELFNIVMNESTIKIEYSSKQKNSI
                     RTIVPIGLYAMNGLWYCPAYCIKSESIKEFRADRIVKILSIENLSNKKYNVLSSIHDY
                     FKNMEVGTDYRIKINLADEGVKHCETEFLLARGLKILSKGGYIDMYIPKSTLNWVAEY
                     FLTFGKNATIIEPIELKHLILFNIFLIMMFYS"
     gene            7285..7572
                     /locus_tag="PC523_003202"
     CDS             7285..7572
                     /locus_tag="PC523_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476692.1"
                     /translation="MNSCWERAVYCNPNGVLKRGVYVLTIKEKDSNNDKDSLVNRSNV
                     YRVNIRLKKETFTEMFGYIPKRPGVGQIVDMDFDFTKLDIVMPHPIYSWMG"
     gene            complement(7851..8450)
                     /locus_tag="PC523_003203"
     CDS             complement(7851..8450)
                     /locus_tag="PC523_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476693.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTRNNEKPNENAGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLITEGERLVKDIDKRIEKLDKVSDEDMKKSQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
BASE COUNT         2733 a         1417 c          805 g         3510 t
ORIGIN      
        1 ataaatttat taaaaatgtt tgtaattcta caaagttatt tttatcaaac taaaaattct
       61 ataaaaaaat aagatacata tccataaaat accttcctat agatacatat cttacctata
      121 taaaaactta ttctatataa aaacgccata aaaaatcttt tgcttcttct gcataatcta
      181 tatttatacg tttactagtt tttatatcta atttatcttt actaaatact ttctttcctt
      241 tatcatcata ataaaaatta cttatatata attcttcacc cattatactc ttactattta
      301 gacttctatc tatttttaat gctttgcaaa gttttcctgg tccattagta atatttttta
      361 cttgatattt actcaactca gtgtaagctt tgttgtatct attcatagat atctcatcca
      421 aagaagttat aggctctacc cctcttatta atacacactc tggaacattt tctatatttg
      481 cagataaatt taagcaatta tacataccat agattaaata aacatatata tgtccaccat
      541 ctaaatataa cggcttcgtt ctatctgttt ttttattgcc atatacatgt gctcctttat
      601 catttacacc catatatgct tccgtctcta tgattttggt aacaattact ttattttcat
      661 atttccttat taggtattta cctaatattg attttgctaa atcaattcca tttttcctaa
      721 aaaaatcctt ttccaaaatt tcacctcata aaatttattt gcaacgcatc aaatacaact
      781 aaagtaataa ataaaattaa ctcttattaa tgtgatatat ataaacaata aattccgaca
      841 tttattattt atatatatca aaaaattcta tctcaaactt ttcaatatta tcattgtaat
      901 ttgaattatc atatacttct tcaacaacct ctttaattgg gaatataatt tttacattag
      961 ttccttctcc taccttgcta tctatataaa tctttccatt ttgaatttca atcaacgatt
     1021 ttgtgaggca taaccctata ccacttccaa attcatttga tatgccactg ttttcaattt
     1081 gtttaaatcg attaaatata tcatttaatt tttctttctc tatacccact ccagtatctt
     1141 ttataattat ctctactgtt tcgtcttttt tatatatacc tacaaggatt tctcctcctt
     1201 ctaaagtaaa ttttatagaa tttgataata tatttagtat tattctttct atagattctg
     1261 ggtcaagccc taccataaca gtttcttcat ctgtatcaaa aattaaatct attcctttag
     1321 acttagcata actactaata gatgtaacta ttctttctgt tatgtcaata atatcaaaat
     1381 taaccgcttt aatcttatat atgtctgact taacttgaga aatatttatt atattatcta
     1441 ttaatctcaa taatctaaac atattttgtt ttgttatttt tatgtgttct tttaatatat
     1501 cctgaaaatc aattattttt ccttttttat aaagattttc aacaagttga actgaactgt
     1561 atatattatt taaaggtgtc ttaagttcat gagatatatt agcataaaac tccatcttca
     1621 tttggtcata ctgtctttgt tttattaatg ctttcttaag tctttcagac tctctttgca
     1681 cacttaaatc tttaataatt ctcactctac ttcttttttg tccaaagtat aatgttcttg
     1741 atatcatttt tacttctttg atgtgattat tttcatttac tatatggata ctcttctctc
     1801 tttctcctat atttaaattg tttatgtatt tttttactaa tatatttcta tatttttgtg
     1861 ttgtaactcc tagtacatca ctagaaattc caaacaactc tctgaaagct ctatttgtat
     1921 ataatactct tgatgtactt aaatcttcaa gaaaaatagc ctctggtata acatccagta
     1981 atttttcgta tattattttt tgttcattta ctgttctaag aatatttatc tctttagtta
     2041 tatctctaaa tgtaagcaat ttaaagagtt tattatctga ccaatagtct ccaactctaa
     2101 cttcttcaac atgctcttca ccttctgaat cagacatcac aaaattaact atacagtttg
     2161 ctgtgtcatt gtaacttact atttcagctt tcttttcttg tgttaaattt aatcttttaa
     2221 attccctatt gtcaaagaaa tctatgttaa aagtttcaaa atattgttct aatgtcatat
     2281 cgactaaatc acttgaagta catttaaaac tcttttcata ttttttattt atacttatta
     2341 ccttattctt ctttataccg ccttcagcac ttcttacaag tataggagat ggaatattta
     2401 gtataatgtt ttcatagtct ttatttatat gtcttatttc ttcatctatt ttgatttcat
     2461 ttgtaatatc atttaaactt attaagtaat tgctttcatc tttattatta cttaaactta
     2521 acaaataaat tttaccattt aactctacta atttatcaat tatacttata tcgttatcaa
     2581 atttcttcaa tttttcagca accttttcat cttcctttat aaaagttaat attttataat
     2641 aattatccat atcctttgaa aattcatctt taaatttatt atttgtgtaa cttatattaa
     2701 agtcatagtc tgttactatc catacatttg gtattgtgtc cagagcttgt ctaagtaatt
     2761 cttttctctg gtctatatag ttttttaatt ttctttgtat aatcatatcc ttttctatta
     2821 ctaaatttat ctttcctaat tgctctgact ttttacttaa ttttttattt attgaatcaa
     2881 gttcgtaata tggatacact accctatttc taagactata tacatataca taataagtat
     2941 ggttacataa taataaatat attactccta taaatctatt tgaatctact atattaatct
     3001 tataaattgc tacaaagcct ataatagttg taaaaaaaat aaaatatgtt aaatctttta
     3061 ttaacttata gtgctcaatg ttactatcaa gcttattcaa caaatatagt aaattaaaca
     3121 aagatataca aacattaatt aatacaataa tcatattcca gttaatatct ctaaaaatat
     3181 ttcttataaa gtaatctaat atcacaaaag cactataaaa aattaatatt gggcttattc
     3241 ttagtttttt tactttagaa atagttaagt atataaaagt agacatatta attatgctta
     3301 aattgagcaa cacaatttgg aatttttgaa tttttaaatt tactactcct aaaatactta
     3361 caaaaactaa tacaagacac cctactttca atatatttat aaattcatct gttttaaagt
     3421 tacttatata aactatagaa tacccaaata tccaaagtaa aattattcta tacatattta
     3481 tagaaactgt atacaaactt atatttaaaa gtgcaataac caaaaataac ccattagcta
     3541 gtatatagac cttattctta cttaaagttc ccttagattc catattcccc accacttctt
     3601 catttttata tagttttacc tataaatatg ttaccacaat tcttccaaaa aatttgtcac
     3661 tttctagtta taaaaaaaaa tgagtaaata tttttaattt tactcatttt gtagattttg
     3721 tatattcaat ttaaaaagct ggaactatac ttcctttgta ttcgtcttgt ataaattttt
     3781 taacttcttt actattcata gcctctgaca aagccttaat cttatcacta tctttattat
     3841 tttctctaca agctattata tttacatatg gagaatcact tgattctatt actattgcat
     3901 ccttagtagg atttaaatta gctgttaaag cataattaga atttattact gctccatcaa
     3961 catcttttaa tactgttggt aattgctctg cattcatttc ttttatttgt atatttttag
     4021 ggtttttagt tatatctttt ttagttataa gctcgccatc ttttacctct atcaatttat
     4081 tttttgctaa taatttaagt gctctagctc cattagttgc atcatttggc acagctataa
     4141 cagctccatc ttttatttca tctaatgctt taactttttc tgaatagaat cccattggct
     4201 ctatatgaac tttagatgta taagtaagtt tatatccttt ctccttcaca gtttcctcta
     4261 aaaatggaat atgttgaaag aagttagcat ctaaagaccc ttcatctaaa gctgtatttg
     4321 gaagtacata atcatcaaat atttttactt ctaaatcgta tcctttttct tttaacaacg
     4381 gttttgctac ttccaatatc tttgcatgtg gatttgatga agctcctact agtatttttt
     4441 tatcctcttt gtttgagcaa cctactgctg atattgctat tgctgacacc aatgctactg
     4501 ataataattt ctttaatttc attttttact ccccctcttt aaaatgttta taattaattt
     4561 attttagttt cttataagcc agatttccta ctccctggat tatctgaact aatattacaa
     4621 gtgctattac agtatatacc attactgctg tatcaaatct ttgatatcca taaataagtg
     4681 ctatattacc tagtcctcca gcccctactg cacctgccat agctgtataa ccaagtatag
     4741 atattattga taatgtaatt ccagatacta ttgaaggcat agcttccttt atcataactt
     4801 taaatactat ttgtcttttt gtagcaccaa aagattttgc tgcttctatc aatcctttat
     4861 caacttcatt taatgagctt tctataatcc tagcgataaa tggtgctgcc ccaatagtta
     4921 taggcactat tgcagcagtt tcccctatgc ttgttccaac gattaatcta gttattggaa
     4981 tcattgcaac tataagtatc ataaatggga aacttctaaa tatatttact ataaatccta
     5041 atgcactatt tattgcgcta tttggtttta atccatctgg ttttgtaact actaatatga
     5101 ttgccaatat aaatcctaat attgttgcca ctatagttgg tactataacc atatacaaag
     5161 tttgtaacaa tgcatttgga aatagtgttg ttaaaaagtc aattaaacta ttcatttatc
     5221 atttcctccc atcttatgcc cttactagtc agatattgtt ttacttgttc gccactttta
     5281 tccgaaatat ttataataag tgaacctaaa atatcatctt taaattgttc taatttccca
     5341 aatattatag acacatctat atttagttct cttgccatag tcgtaataat agcttcatta
     5401 gaaatatctt tagggaatag tatctttata tttgttcctt ttggtaatat tatactttct
     5461 tctcctatta attttctaag acctttagta tttcttaaga atatttcttc tgtatctcct
     5521 acctctaata cttctccatt ttccattatt gctactcttc cacaaatctg ctttatgacc
     5581 tccatttgat gggttacaac tattattgtt attcccaatt ttttatttat atcttcaagc
     5641 aatgaaagaa ttgattttgt agtatttggg tctaatgctg aagttgcttc atcacaaaga
     5701 agtacctgtg gatttagtgc taaagctcta gcaattgcaa ctctttgttt ttgaccacca
     5761 cttagatttc taggtttatc atttttcttt tcagatattc caaccacttc taataactct
     5821 aaaactcttt ttttaatttc tgcctttgaa tatccaaaac actctaatgg caatgctaca
     5881 ttatcaaata cagttcttct ttcaagcaat gaaaaatgtt gaaaaatcat tcctaactct
     5941 tttcttaaat ctctcatttg cttttcattc aatgatttta cttctttgtc agaaacaagt
     6001 acattacctt cttggtattc ctctaatccg tttatacatc taagtagtgt tgatttacct
     6061 gcaccactat gacctattat tccaaaaatt tctccagatt ctatttcaat actcacatct
     6121 ttaagcactt gaatctttcc ataatactta ttaacatttt ttatgctaat cataaaattt
     6181 cctcccatat attctgctca gtttttatgt ttatcctagc ttatgtatta caacaaaata
     6241 aaagcccgag tataaactca ggcttagaaa gctatttata ctcatctatc aactatatta
     6301 gttgcaggat ttagcaccaa tgtatacaaa atatactggt tgccgggttt caaagggcca
     6361 gtccctccac cactcttgat aagacgttat ttaatttaat tctattatca tttgttgcta
     6421 tgttatatat aatatattca ttaatagtat ttgtcaataa atttatttaa attttttatt
     6481 ttttattatt gttaattaac tatttttaat tagttttcta gctcctaaca ctttatattt
     6541 taatgtctat ctaactatag aacatcatta taagaaatat gttaaatagt atcaaatgtt
     6601 taagttctat aggctctatt attgtagcat ttttcccaaa ggttaaaaaa tattcagcca
     6661 cccaattaag tgtagatttt ggtatataca tatctatgta tccaccttta gacaaaattt
     6721 tcaatcctct tgccaacaaa aattctgttt cacaatgttt tactccctca tctgccaaat
     6781 taattttaat acgataatct gtgcctactt ccatattctt aaaataatca tgtatagaag
     6841 ataaaacatt atatttctta tttgataagt tttctatact caaaatcttc acaattctgt
     6901 ctgcacgaaa ttccttaata gattctgatt ttatgcagta agctggacaa taccaaagtc
     6961 cattcatggc atacaaacca atagggacaa tggtacgtat actatttttt tgctttgatg
     7021 aatattcaat tttaattgta gattcattca ttactatatt aaacaattct ttcaataatg
     7081 gtgttgaaca atgcatatct ggtgtccaaa aaacaaattt acgcctcata ttctcaatat
     7141 tatattaaat atcatttggt atacaattta atttttttta aaacagaaat agtttcttgc
     7201 tcaaacggca aatcactata agatactatt aaggctgatg atattatcaa atattgtcta
     7261 tcaaacttag atgatgttgt actaatgaat agttgttggg aacgagcagt ttactgcaat
     7321 cctaatggtg ttttaaaacg aggagtatat gttcttacca ttaaagaaaa agacagtaat
     7381 aatgataaag attcgttggt taatcgttca aatgtatacc gtgtaaatat acgattaaaa
     7441 aaagaaactt ttactgaaat gtttggatac attccaaagc gtccaggtgt aggtcaaata
     7501 gttgatatgg attttgattt tacaaaattg gatatagtca tgccacatcc tatctactca
     7561 tggatgggat gaatatgcat cctaagccct actgaaaaga catttaagaa ccttaaaact
     7621 ttaataggag aatcttacaa ttttgcaaaa gaaaaattca aaaagagaaa aaacaagtaa
     7681 aaacatatga aaataacctc taaataaatt ataataaaaa tattaaggtt acaagataaa
     7741 aaattaatat cttaataata tacaaaaaag cgaagccaaa aaatgtatta tttataaggc
     7801 ttcgctatat ctcaaataat ttatttcaaa ttttaaaact atttattcta ttatttctta
     7861 ttatttcctg ttattcctaa tctatcattc atatctttta tcatttttct aaaaccacta
     7921 cctatatcat ttccaacatc ttcagttcca tgaactaatt ttataaagtc attttgactt
     7981 tttttcatat cttcatcaga tactttatct aatttttcta ttctcttatc tatatctttt
     8041 actaatcttt ccccttcagt gattaggtca tcattcattt tctttaactc tgtatcttct
     8101 gttttaccat cttttttaag agatgttaca aatgattgta ttttatctct actatcttta
     8161 tatgcagcct ttacatcact tagatatttt tcatttcctg gatattcttt gtctttaaac
     8221 tgattattta tactttctgg agtatcataa atagagtatc tatttagacc tgctatataa
     8281 tcattatagc ttgttgaata ataattcata tatccttcat tattatttac agattcatta
     8341 ggattttttc ctgcattttc atttggtttt tcattatttc tagtacatgc agttatacct
     8401 atagctaata ctaatgctaa tcctacaaat aaagttcttt ttaatttcat ttttattacc
     8461 ctcct
//
LOCUS       DAMJVM010000129         8443 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.134, whole genome shotgun sequence.
ACCESSION   DAMJVM010000129 DAMJVM010000000
VERSION     DAMJVM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8443
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.134"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            3..2180
                     /locus_tag="PC523_003204"
     CDS             3..2180
                     /locus_tag="PC523_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2476694.1"
                     /translation="MYNKCNNLYLLLGKENINMDSYRQLKAKIPCKETGIEIRRTLCD
                     ICSPGVHCGIDAYVKNGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKTISWDEAYREIAEKLNKVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLT
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQRDLIDKEYIE
                     KHIHGFNEYKEYVKLFDLDTVSRITRVSKDNIVKAVEMLSQGSFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALDSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANVMNIDDDLLKAGYEASIRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTYLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKTNR"
     gene            2194..2616
                     /locus_tag="PC523_003205"
     CDS             2194..2616
                     /locus_tag="PC523_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2476695.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GESIEYFSLACIHCDEPKCLDVCPKNCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGMDGKANKCNGCVERYKCGMDSPCVMVCPTGALKFNK"
     gene            3024..3827
                     /locus_tag="PC523_003206"
     CDS             3024..3827
                     /locus_tag="PC523_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2476696.1"
                     /translation="MKKIKLIIMLALVSAFLITGCSSENNKNKEEQKTIRVATSGTYY
                     PFAFKEKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYDFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKLVEPPIQFGAC
                     SFALQKNEKGERWTKEINKVIEDMHKDGTLTKISKAWLGKDITKEPINE"
     gene            3820..4488
                     /locus_tag="PC523_003207"
     CDS             3820..4488
                     /locus_tag="PC523_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2476697.1"
                     /translation="MSSFNFEFCLNIFPFLIKALGITILISIIAFFISILLGVILAFF
                     TQTKSKILQYITRVYVSFFRGTPLLIQIFFFYFGFPMVFSFMKDCSPYLAIVICLGVN
                     SSAYMAEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMVTILIYWAVSIMLSNIQSKVEKRI
                     GKAY"
     gene            4723..5385
                     /locus_tag="PC523_003208"
     CDS             4723..5385
                     /locus_tag="PC523_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2476698.1"
                     /translation="MIFNMKYFLELFPIIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMSDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDKGQIEAAYAMGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            5445..6242
                     /locus_tag="PC523_003209"
     CDS             5445..6242
                     /locus_tag="PC523_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2476699.1"
                     /translation="MIKRNIIKCLVAVLSLTVLVTGCSKKEEVKDEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVAASVNKD
                     NLFEEKVYGDGTNGLQDLDLGRVSAWIDAELTVIGDAKKANMKVRVLDEKLSVTNIAY
                     FFKKNDEKSKTIKEDVNKAIEEMLADGTVKKISEKWFGMDVSADIQK"
     gene            6375..7550
                     /locus_tag="PC523_003210"
     CDS             6375..7550
                     /locus_tag="PC523_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2476700.1"
                     /translation="MIETKIKSIALKNREKIIKIRRQIHSNPELAFKEFKTSKLIKEE
                     LNKLNIEYIDVAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSINDNMHA
                     CGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGVDALAT
                     GHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAVYSSIQ
                     QIVSRKINPVIPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISQEKVIEISKILENIV
                     RGVCKSNGADCKFEKSMGGDAVINDKDMIELGKKSACKILGYENVEMIDFPAMTGEDF
                     AIYMKERPGLFMYIGVGSEEKNINYRLHNNKFDIDEKCLSVASSLFSQLAVDYLCQL"
     gene            7659..8408
                     /locus_tag="PC523_003211"
     CDS             7659..8408
                     /locus_tag="PC523_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF2476701.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEINQSEVVAILGPSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVERTTKKDIANLRKHTSMVFQNYNLFKNKTALENIM
                     EALIVVHKKGKQEAREISLKLLDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDKGVIA
                     EEGTPDEIFNNPKNERTAKFLNIIKKEEVFS"
BASE COUNT         3351 a          864 c         1573 g         2655 t
ORIGIN      
        1 gaatgtataa taagtgtaat aatttatatt tattattagg aaaggaaaat attaatatgg
       61 atagttatag acaacttaaa gctaaaatac catgtaagga aacaggtata gaaataagga
      121 gaactttatg tgatatttgt tccccaggag tacattgtgg aattgatgca tacgttaaaa
      181 atggtaaagt aataaaaata gaaggtacaa aagaacatcc aatgaataaa ggattgttat
      241 gtacaaaagg tctttgcaat agagaatata tctatagaga agatagaata aaaacacctc
      301 taaaaagaat tggaaaaaga ggtgaaggaa aatttaagac aatatcatgg gatgaagctt
      361 atagagaaat agcagaaaaa ttaaataaag taaaatctga gtatggagca aagcatgtag
      421 ctttttttac aggttatgga aagtggtata gaccgttttt acaaaggttt gcatcttcat
      481 ttggttctcc taattatggt acagaatcta gttcatgttc aacatcagca cagatggcgg
      541 gaaatgttgc tactggaaga aactttacaa tgcaagatat ggagaatgca aaaacttact
      601 taggatgggg atttaatcca tactactcta aatttttaaa tagtgtaaat ctaacaaaat
      661 taaaagaaaa aggtctaaaa tttataattg tagaccctag gataacacca acaacccaaa
      721 aactagcgga tttacattta agaattaaac ctggaactga tggggcttta gctcttggaa
      781 tggcgaatct atttatacaa agagatttaa tagataaaga atacatagaa aagcatatac
      841 atggatttaa tgaatataaa gaatatgtaa aactatttga tttagatact gtaagtagaa
      901 ttacaagagt ttctaaagac aatatagtaa aagctgtaga aatgttgtca caaggttcat
      961 tttgtataaa tgaaagtgca gctccaatag ttcatcatca aaatggattc caaaactata
     1021 gagcaatgat gtctttatct gctataacag gcaattatga tagaataggt ggaaacatac
     1081 caaaacaaga ttactttgct ggtaagatag gtggattcca aacactagaa aaagagtttg
     1141 taaaagaagt tctacctaaa aatataaatg aaagaattgg tggagaaaga ttccctctat
     1201 ggaatgaaat ggtaactcaa tttcaagcta tggatttacc taggcaaata gaagaaaaga
     1261 caccatatga aataagagct ttgttttgtc atgggataaa tgctagaatg tttcctaatt
     1321 cagggagact ttttaaagca ttggatagtg tagatttttt tgtgaatata gatttattta
     1381 tgacagatac atcaaaatat gcagatatac ttttacctgc atgtacatca ttcgagagag
     1441 aagaatgtaa gagctatcca ggagggtata ttacatatac caaaaaagtt attgataaat
     1501 tatacgattc aaaatcagat gtagaaatac tttcagattt agcaaatgtt atgaatatag
     1561 atgatgattt attaaaagca ggttatgaag caagtataag acatatgttt aaaaatacgt
     1621 gtgtagatgt agataaactt aaagaaagtg atttaccatt aaaatgtaaa gattttaagc
     1681 catatatagt aggttcatat acaagagaag ggtatgatac tagtacttct aggtttgaat
     1741 taaaatctac aattattgaa aaatataaag attttgggct ggatgcacta ccaacatata
     1801 gaggatttaa tgataatata gatgatgaat atacttattt attgacatca ggagcaagaa
     1861 tttcaaatgc actacattca agacttcatg acatcaaact ttcaagaata ttaaaaaatg
     1921 aagctagttg tgatatttct agcttagatg caaaagaatt gggtgttatc agtggagatt
     1981 atataaaaat aactactaag ataggaagtt tggatgtaaa agtcaatgtt actgatagag
     2041 tcttatataa gaatatacat atgtatcatg gatatagaga agctgatata aatagtatta
     2101 tttgtgataa ttttgaccct tatagtggat tccctgcgta tagaagttgc aagtgtaaga
     2161 ttgaaaaaac aaatagataa atggagagat ataatgaaaa gtttatttaa atttaataaa
     2221 gaattatgta cagcatgtgg agcatgtttg cttgcatgta tagaccaaaa tgataataat
     2281 atagataatg gtgaactttt tagacatgta tttacaatag aggaaggtga aagtatagaa
     2341 tatttttcat tagcatgtat acattgtgat gagccaaaat gtttagatgt atgtcctaaa
     2401 aattgtttta aaaaagaaga aggattcgtt gtattagata atcaaaactg tatagggtgt
     2461 aaactttgtg aaaaagcttg tgaatatgga gccctaattt atggaatgga tggtaaggca
     2521 aataagtgta atggttgtgt cgagagatat aagtgtggta tggattcacc ttgtgtaatg
     2581 gtatgtccta caggagcatt gaaatttaat aaatagatta aatatataga gtgacttata
     2641 cttagatata agatattttt acaaaaagct atattgagtt tgttataata aaaactcaat
     2701 atagcttttt gtatggatat agttggatac tataatatga ttcttagtaa tagttttttg
     2761 ctattggaca aagactgtaa aacatattaa acttatgttg ttcaaagtcg aagtataata
     2821 taataactag aaaattgatt tatagtgtta taaaagcaaa tttaggaagg tataaaatct
     2881 gaagtaattc tttactacag tatcaatact tggagttctg ttatggcaga gtcattaaac
     2941 aattataatg gtacaagtga ttcaattgct ggaaatgtat tatataaaaa tttaaaaaaa
     3001 gattaaaaag gagagaataa attatgaaaa aaattaaatt aattattatg cttgccttgg
     3061 tatcagcatt tttaattaca ggatgctcat cagaaaataa taaaaataaa gaagaacaaa
     3121 aaactataag agttgcaaca tcaggaacat attacccttt tgcttttaaa gagaaagata
     3181 aacttaaagg atttgaagta gatttttggg atgaatttgc taaacgaact aattgtaaag
     3241 ttgaatgggt attaactgat tttagtggat tatttggatt attagaagct gataaagcag
     3301 atgttgtatc tgctcaactt actgcaacac ctgaaagaga gaagaaatat gatttttcag
     3361 atgcttataa ctatagtgga acaaatatta ttgttagaga agatgatact agtataaaat
     3421 caatagatga tttagttgga aagaaagtag gtgtgggtac tggagcagta gcaaatgaaa
     3481 tactaaaaga aaagtaccca aatgatgaaa taaaaatagt aaattattca agtgcaactc
     3541 ttaaaggaaa ttatcaagac ctagaaatgg gaagattaga tgcagtagtt gcgcaggatg
     3601 ttgaggcttt aatagctata aaagaacaaa aattaaatct taaattggta gaacctccaa
     3661 ttcagttcgg agcctgttcc tttgcgcttc aaaaaaatga aaagggtgaa agatggacaa
     3721 aagaaataaa caaagtaatt gaagatatgc ataaagatgg gactttgact aaaatatcta
     3781 aagcctggct aggaaaagat attactaagg agcctattaa tgagtagttt taattttgaa
     3841 ttttgtctaa atatatttcc atttttgatt aaagcattgg gaataacaat actaatatct
     3901 attattgcat tttttatatc aatattatta ggtgtaatac ttgcattttt cacacagaca
     3961 aaatcaaaaa ttttgcaata cataactaga gtgtacgttt cattttttag aggaacacca
     4021 cttttaattc aaatattttt cttttacttt ggatttccaa tggtattttc ttttatgaaa
     4081 gattgttccc cttatttagc aatagtaatt tgtttaggtg taaattcatc tgcatatatg
     4141 gctgaaatta tacgagcatc tattgagagt gttgataaag gacaacagga agcatgttat
     4201 gcttttgggt taagtaaata tcaatcaatg gttaaaataa ttctacctca agcagctaca
     4261 tctgcagtac ctcctattgt aagttgtaca ctagatataa ttaaaggttc ttctttaggt
     4321 ctaactatag gaatacaaga tttaatggga gtagctcaag tagaggctgc ttcaagtttt
     4381 aaatattttg agatatatat ggtaacgata ctaatttact gggcagtatc tataatgttg
     4441 agtaatatac aaagcaaagt tgaaaaaaga ataggaaaag catattagat ttcaatattt
     4501 aaaaatggga tgatatagat atattatcac ttgtaaataa atttttaatt ttagaaaaac
     4561 aaatttagta attctaaata ttgagaaaat ataatacaaa atgaacttta taatcaagtt
     4621 gaaaaatggt tttttctatt gaaaaataaa taaaattcta ttgtaattta atatgtgaca
     4681 aaagtttgta aaaaacaaca ttaagctggg gggacttatt ttatgatttt taatatgaaa
     4741 tattttttag aattatttcc aataataatg aaatcgttca atgtaactgt gattttggca
     4801 ataagttcat tagtattttc attaattata ggtacaatag tagcattatc atcttattac
     4861 aaagttaaag tattgaatca aatatgtaaa gtatatgtat ctatctttag aggtacacct
     4921 cttatgccac agttattctt tttatatttt ggattagcat atatgagcga ttttgttaaa
     4981 aatatggacc cagtatttgc aacatctata gtgcttagtt taaatatggg agcttatatg
     5041 tctgaaacga taagggcttc tatactttca attgataaag gtcagataga agcagcatat
     5101 gcaatgggat taacaaattt acaaacaatg aaaaggatag tgataccaca ggctgtaaga
     5161 gttgcactgc catcattatt caataatttt atagatttaa ttaaagggtc ttctgtagct
     5221 tttgttgttg gagtaaatga cataatgggt gctgctaaat cacaaggagc gttgagtttt
     5281 aagttctttg aagtgtatgg agcagttatg gtaatttact ggtgtataat aactccttta
     5341 gggtgggggc aaaaaaaatt agaaaagaaa ataagcaatt attaagttat taagaaaata
     5401 cataaaagta tttttaaata aaaaatggtg tgaggagaag agatatgata aaaagaaata
     5461 ttataaaatg tttagtggca gttttatcgt taacagtttt agttactggt tgttcaaaaa
     5521 aagaggaagt aaaggatgaa aaagttataa ctgtagcttc atctaattca gctgacccat
     5581 attcttatgt tgataatggt gagcataagg gttttgaagt tgacatgtgg aaagagattg
     5641 gtaaaagaag tggatataag ataaaaatgg aaccaacagg ttttagttct atatttggga
     5701 tgttagattc tggtaaagtt gatgtggcag ccaacttctt tggaatgtct aaagaaagat
     5761 tagaaaaata cgatgcttct ataccttatg gttctgattc tgtatgtata gctgtaaaag
     5821 atggaaacaa cgaaattaat aaacttgaag atttaaaagg aaaagttgta gcagtttcag
     5881 aaggttctca agggcaacag gttgcagcta gtgttaataa agataattta tttgaagaaa
     5941 aggtttacgg tgatggaact aatggtcttc aagatttaga tttaggaaga gttagtgcat
     6001 ggattgacgc agagcttact gttataggag acgctaaaaa agctaatatg aaggtaagag
     6061 tattagatga aaaattatca gttactaata tagcatactt ctttaagaaa aatgatgaaa
     6121 aatctaaaac tataaaagaa gatgtaaaca aagcaataga agaaatgctt gcagatggaa
     6181 cagtgaaaaa aatatctgaa aaatggtttg gaatggatgt aagtgctgat atacaaaaat
     6241 aaatacttta aaattaaaat ataaaactgt gataactata gataagtata ctaaatttat
     6301 ctatagttat ctgtgtatta caatgtattt agattagtct ttataaatta aaatgagtta
     6361 tggaggtctt gcaaatgatt gaaactaaaa taaaatccat tgcactaaaa aatagagaaa
     6421 aaattataaa aataagacga cagatacaca gtaatccaga gttagcattt aaagaattta
     6481 aaacatccaa acttataaaa gaagagctaa ataaacttaa tatagaatac atagatgttg
     6541 ctggcacagg agtattagct actataaaag gaaaaaataa tggtgggaaa actatattac
     6601 taagagcgga tatggatgcc cttccaatca aagaggaaaa tgatttagaa tttaaatcaa
     6661 taaatgataa tatgcatgct tgtgggcatg atgcacatgt ttcatggttg ttaggaacag
     6721 caatgatttt aaatgacata aaagaagaat tgaatggaaa tgtaaaatta ctatttcaac
     6781 ctggcgaaga aaaaggtggt tctgatattg taattaaaga aaatgtactt gaaggtgtgg
     6841 atgcattagc gactggtcat tgttggccaa ctattgaatc aggaaaaatt ggaatcgcta
     6901 gaaattgtgc tatggcagcg actaatactt ttgaaataac aataattggc aagggtgggc
     6961 atggagcaga gcctcataat tgtatagacc caattgcagt tggaaatgca gtatattcat
     7021 ccattcaaca gatagtttca agaaaaatta acccagttat acctgtagta gtgtctgttt
     7081 gctcttttaa ttctggtgtt tctaaaaata taataccaga tgtatgcaca cttcaaggaa
     7141 ccattagagc gataagtcaa gaaaaagtaa ttgaaataag taaaattcta gaaaatatag
     7201 taagaggtgt ctgtaaatca aatggagcag attgtaagtt tgaaaagagc atgggagggg
     7261 atgctgttat aaatgataaa gatatgattg agttgggaaa aaagagcgct tgtaaaatac
     7321 ttggatatga aaatgttgaa atgattgatt ttcctgcaat gacgggtgag gactttgcta
     7381 tttatatgaa agaaagacct ggtttgttta tgtatatagg cgtaggaagt gaagaaaaaa
     7441 atataaatta cagattgcat aataacaagt ttgatataga tgaaaaatgt cttagcgtag
     7501 catcttcttt attctcacaa cttgcagtag attatttatg tcaactatag agtacattca
     7561 taaaaatata tttagcctat ttttaaattt aatgctagta tattaatttt aatgtagaaa
     7621 atattattta ttatatttat ttaaaaaggt ggataacaat gataaaagta gaaaatttac
     7681 ataaaagttt taaagggaca gaagttctta aaggcataaa tctagaaata aatcaaagtg
     7741 aagtagtagc tatacttggc ccatctggaa caggtaaatc tacattgctt agatgtttaa
     7801 attatctagt tgaacctaca aaaggaaaaa taatgattgg ggatgtaaag gtagatgtag
     7861 aaaggacaac gaaaaaagat atagctaatt taagaaaaca tacatctatg gtgtttcaaa
     7921 attataactt atttaaaaat aaaacagctt tagaaaatat aatggaagca ttaatagtag
     7981 tacataaaaa gggtaagcaa gaagctagag aaatatcttt aaaactatta gataaagtag
     8041 gtatgttgga taggaaagat ttttatccat caaaattgtc tggtgggcag caacaaagga
     8101 taggtatagc aagagcgatg gcagtaaatc caaatgtaat attatttgat gagccaacat
     8161 ctgcacttga cccagaactt gttggagaag ttttaagcgt tataaaatcg cttgcaaaag
     8221 aaggtataac gatgcttatt gtaactcatg aaataaattt tgcaaaagaa gttgctgata
     8281 aaattatatt tatggataag ggcgtaatag ctgaagaagg aactcctgat gaaatattta
     8341 ataatcctaa aaatgaaaga actgcaaagt ttttaaatat cattaaaaaa gaagaagtat
     8401 ttagttaaaa gtatataaat taagtattag aagatatgta aga
//
LOCUS       DAMJVM010000130         8394 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.135, whole genome shotgun sequence.
ACCESSION   DAMJVM010000130 DAMJVM010000000
VERSION     DAMJVM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8394
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.135"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            1..117
                     /locus_tag="PC523_003212"
                     /pseudo
     CDS             1..117
                     /locus_tag="PC523_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888701.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            118..873
                     /locus_tag="PC523_003213"
     CDS             118..873
                     /locus_tag="PC523_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2476702.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTSPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALQYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            1004..2026
                     /locus_tag="PC523_003214"
     CDS             1004..2026
                     /locus_tag="PC523_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2476703.1"
                     /translation="MINKKRLASLILAGALSVSMLTGCSQGGDSGNSKQESNSKSKEV
                     KKIGITQLVEHPALDATKAGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILMTAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNKNISY
                     VKTKQE"
     gene            complement(2188..2745)
                     /locus_tag="PC523_003215"
     CDS             complement(2188..2745)
                     /locus_tag="PC523_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2476704.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEGEYGK
                     REAILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APSVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGVTIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(2772..3827)
                     /locus_tag="PC523_003216"
     CDS             complement(2772..3827)
                     /locus_tag="PC523_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HDF2476705.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISQLNRIDKNTIYAIDWGCELTLDREIVDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIIENPFTYTMYVRKTNLSNECVPIQIELEKSKMRFKK
                     GDSIYLDFPQEYLFCFK"
     gene            complement(3828..4511)
                     /gene="modB"
                     /locus_tag="PC523_003217"
     CDS             complement(3828..4511)
                     /gene="modB"
                     /locus_tag="PC523_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDF2476706.1"
                     /translation="MGTDWSPLWISLKTALLSTVITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLSFDKTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKVAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVVISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4660..5472)
                     /gene="modA"
                     /locus_tag="PC523_003218"
     CDS             complement(4660..5472)
                     /gene="modA"
                     /locus_tag="PC523_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2476707.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNESKKDDGKQEKTNKSSDSIE
                     LNVSAAASLKEAMAKIEEEYKKIDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSNVTGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSEEGQKIFEEFGYKKVE"
     gene            5665..6594
                     /locus_tag="PC523_003219"
     CDS             5665..6594
                     /locus_tag="PC523_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2476708.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     IKDVEEYINIQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEIKTWDDLTKSNITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGIGGYDLTDTGKTIAKV"
     gene            complement(6756..7205)
                     /locus_tag="PC523_003220"
     CDS             complement(6756..7205)
                     /locus_tag="PC523_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476709.1"
                     /translation="MKNKNNKDKNMFNIKVLRVLGLLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGADCIVFLLFIVECVYLVGRYILKVKINWSDIIESEKDNFINNLLVEKI
                     QEESNIVYNLLNSYVLPYIYKKGKGLMSKTYHMLIYIKEKYYYVNIY"
     gene            complement(7407..8387)
                     /locus_tag="PC523_003221"
     CDS             complement(7407..8387)
                     /locus_tag="PC523_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2476710.1"
                     /translation="MKNSFKNYISLRNKFIIISIVFLLILLILSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLDKLKNYKTAELMIQSSNNYKLESLFITSNIKTRDTII
                     LVHGIGSSYYEMLKVAYGYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHTEINSKNEKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPGILIDYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKELWFIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         2927 a         1372 c         1009 g         3086 t
ORIGIN      
        1 taataactgc catagttata atagctttct tagctactgg taacttgaat atttcactaa
       61 aaaaaaacaa atgcaaagct agtaccaaaa ataaatcaga aaaaagaggt gttgtagatg
      121 ttacaaatta aaaatctttc aaagagtttc cccaatccat acggtgaacc taatactata
      181 tttgaaaatt tatcaattga tatagaagat ggtgaatttg taagtatcat aggtagtaat
      241 ggaactggta aatctacttt attaaatata atctctgggc ttataaagga atcttctggt
      301 caagtattat taaataaaca taacttgtct aatttagctg aacacagaag aactcaaata
      361 gtaagtagag tttttcaaga cccaagtctt ggaacttgtc cttctatgac tgttagggaa
      421 aacttgtctt tagctcttaa taaaggaaaa ctattaaacc taagaaaatg tcttcgttac
      481 aaaagagatt ttttagaaaa tttattggaa ggtgtatctc ttgatttaaa aaaatacctt
      541 gatgttcaag taaaattttt atcaggaggt caaagacaat cattatcact aattatgtcg
      601 tgcttaacaa gtccatcagt tttattactt gatgaacata cagcagcact tgacccaaaa
      661 acatcaaatg aagtaattga attgactaat aagattgtaa gggaaaaaaa tataactaca
      721 cttatggtta ctcacaactt aaaacacgct cttcagtatg gtgatagatt gataatgctt
      781 cataagggtg aagttgtatt agatgtaaaa ggtaaagaaa aagaacaatt aacagttgaa
      841 gaaatcttag aaaaatttga atatgcagtt taaaataata tatctattgt attaaactca
      901 gctttaaaat aaagttaaaa ctataataat tatggttact ttacaataat tcatatgaat
      961 atatgaacat atataataat aaatttttga gggggatata aaaatgatta ataaaaaaag
     1021 attagcaagt ttgattttag ctggtgctct tagtgtatct atgcttacag gttgttcaca
     1081 aggaggagac tctggtaatt caaaacaaga aagtaattct aaaagtaaag aagttaaaaa
     1141 aattggtata actcaactgg ttgaacatcc agcattagat gcaactaagg caggttttgt
     1201 aaaagcatta gaaaagaacg gatttaaaga tggagaaaat attgatatag acttccaaaa
     1261 tgctcaaaat gatatgccta ctacacaaag tattgcaagt aaatttgcat ctgacaagaa
     1321 agatttaata tttgctatat ctactccatc agctcaggca gcatttaatg ctactaaaga
     1381 tatacctata cttatgactg ctgtcagtga ccctgttgct gctggattag ttaaaactct
     1441 tgaaaagcca ggcacaaatg tatctggaac atcagatttt gtttctgtag ataaaggttt
     1501 agaacttctt aagatatttg ctcctaaggc aaaaactata ggagttatgt acaatactag
     1561 tgaagtaaat tccaaagttc aagttgatgc tttaaaagaa tatgcatcta aaaatggctt
     1621 taaagttgtt gaaaaaggta taactacttc aaatgaagtt aatcaaggta tttctagttt
     1681 agttggtaaa atagatgtat tatatgttcc tacagacaac ttagtggctt cttctatgcc
     1741 tatagtttct aaaatagcta ctgagaataa aatacctgtt atagcagctg aatctggtcc
     1801 agttgaaaaa ggtgctcttg cttgtcaagg tatcaactac gaaaaactag gttacaaaac
     1861 aggtgaaatg gcggttaaaa ttttaaatgg cgaatcagta tctgacatgc ctgtggctac
     1921 atcagatgat acagatatca ttgttaatga agatatatta aaagctttag gtatggaaaa
     1981 accttctaat aaaaatattt cttacgtaaa aactaaacaa gaatagaaag atattttcta
     2041 tatacgatta atatgtccgt ttctataaat taatttatag aaacaggcat atatttttgt
     2101 tttaatgtat tatttattta aagattatat aaacgctctt aatttatttt tataaatttt
     2161 acttgtataa actttgtaca atcatattca ttcttctatt ttctttatta ctctacatgg
     2221 atttccaaca gctacagtat ttggtggtat atcttttgta actacacttc ctgcacctat
     2281 tactacatta tcacctatag taactcctgg tgttatgatt acgcccccac ctatccatac
     2341 attatcacct atttttacag gagagccata ttctatacct gaatttctaa gttgtgcatc
     2401 aatcggatga taggctgtat agatttgaac gctcggagct aacattacat tatcacctat
     2461 ttcaatctta caaacatcca agaaaataca gtcataattt gcaaagaaat tttcacctac
     2521 atgtatgtta tatccataat cacacctaat gttttgttct acatttattt gctttccaac
     2581 tgaaccaaat agttgtctta aaatagcttc tctttttcca tattcaccct ctaaagtatt
     2641 attaaatagt ctagttaatt ttttacaata ctctctttct tttactagca attcatcatt
     2701 tgcatagtaa cctttcccag acaacatttt ttctttttcg gtcatataat ttactccttt
     2761 ttattgtaat tttacttaaa gcaaaataaa tattcctgtg gaaaatctaa ataaatgctg
     2821 tcacccttct taaatctcat tttacttttt tctaattcta tttgaatagg tacacattca
     2881 ttagataaat tagtttttct aacatacata gtataagtaa atggattttc tataatatta
     2941 atcaattcaa aacataactt ttcatttaaa tcttcattag aatccaagac cttaatgtga
     3001 tgctctctaa ttcccacata ttctatatta tcaactattt ctctgtctaa tgtcaactcg
     3061 catccccaat ctatagcata aatagtattt ttatctattc tatttaattg ggatatattt
     3121 ttgcaccctg ttatcttagc ttctataagg tactttggag attcaaagag ttctttttta
     3181 ggtctctttg gaagaccttc tcccttgtta tacactataa tgtcatcgca aactcggtag
     3241 gcttcttcaa tatcatgagt aacaaatagt atatctccct tataatcttt taacatattc
     3301 ataagttctt tttccatatt gcttcttaag tgatggtcca gtgctgagaa aggttcatct
     3361 aaaagtaata tatctggaga tgttgccaat gctctagcta gtgctaccct ttgttgttga
     3421 ccaccagata attgccaagg gtatctaccc tcaaacccat ttaatttaag tatatctaaa
     3481 taccttgcta ctatttcctt tttttcactc ttgcttagtt ttaaaagccc aagttctata
     3541 ttttgactta cagtcatatg tggaaacaaa gcatagtttt gaaataagaa acctaccttt
     3601 ctatcttgtg ttgataaatt tatcttcttt tcagaatcaa atagaactct atcatttaat
     3661 acaattttac ctcttgttgg cttctcaaga cctgctatac attttaatgt catacttttt
     3721 ccagaaccag attctcccaa aaaccccagc gtaccttttt cttgtttaat ttctaccttt
     3781 aatttaaaag aggataaatc tttttcaata tctacgtata agctcaacta tccacacctc
     3841 tttcctataa ttttctgctg aaattccgac caataattta gtaataaaat cattgctata
     3901 gatattacta caataatcat aacccacatc atagctttat tcatatctcc accttcaaca
     3961 gcaaaaaata tagctattgg catagtctga gtttttccag gtatatttcc agctatcata
     4021 agcgtagcac caaactcacc aagagctctg gcaaatgaca gcactaatcc accaattatt
     4081 ccaggccatg ctaatggaac tgcaacctta aaaaatactt tccattcatt aagacctaat
     4141 gtcctagcag ctgacaacat attattatca atctgttcaa aagctgacct tgaagttcta
     4201 tacatcatag gaaaagatac cactactgct gaaattacag ttgctgtcca actaaatatt
     4261 aaagtcttgt caaaacttaa taaaaacttc cctataaatc cattcttccc acataacaat
     4321 aataaaaaga atcctacaac tgttggtggc aaaattagtg gaagtgtaaa tagtccatct
     4381 ataagaccct tccacttacc tctatagtta gccataatat atgacacaaa tattcctatt
     4441 acaaaagtta ttactgttga caaaagagct gtcttaagtg atatccataa aggtgaccaa
     4501 tctgttccca tctttccctc cttaatttta aatggaaata gtgaaattaa taactctatt
     4561 aatttcacta tttccatttt attataatac acaattattt tcaattttca tttatttata
     4621 tataatttac actcaattat acataattta tttataagtt tattctactt ttttatatcc
     4681 aaattcttca aaaatctttt gcccttcctc acttagtaaa aattcttcaa atttcttagc
     4741 agcatctaca tttttactag ctttaattac cgaaactgga tatgtaatag gtgaatgagt
     4801 tttttcgtct gttttttcaa ctacttttat cttatcatta ttgacagtat cactgaagta
     4861 aacgaatcca acatcagcat ttcctgattg tacccatgct aaaacttctt ttacatcttt
     4921 tgcaaatact agcttatctt ttaacttatc ttttaaattt aaattagtta acacctcatc
     4981 tgcatactta ccagctggaa cactttcagc ttcaccaaca gcaattttct taactttatc
     5041 tgtagtcaaa tctttcatac cagttacatt actgtcagca gaagaaataa gaactaaatc
     5101 attttttact aaatctttca tagtatcaga aactaataac ttctcttcat ccaaagcttt
     5161 catttgtttt tgacctgctg aaataaataa atcacaaggt gctccttgct ctatttgttg
     5221 ttgtaatgag cctgatgctc catagttaac tgtaagcttt acatttgaat caattttttt
     5281 atattcttct tcaatctttg ccattgcttc ttttaaactt gcagccgccg atacatttaa
     5341 ctctattgaa tcactacttt tatttgtttt ttcttgtttt ccatcatcct ttttgctttc
     5401 attactattg catccaactg tccccaaagt taaacatgca actagtccta atattcccaa
     5461 tatttttttc ataccaatct ccctttttgt aaatttaata catattttaa catgttttat
     5521 tatgttttat catgttttgt catgtatagt ttaattatat atacctgata ataaattttc
     5581 aagtaattta tttacattac ttttaaatat tttgctacat tttccttttt ttaatgttaa
     5641 aataacctta tatggaggtg gattatggac tcattatcat ctttgacacc tgtcgaggta
     5701 gctgaattat taaaaataac aaaaaatact gtctatgaat taatcaaaag aggtgaactt
     5761 ccttcttata aagtagggaa aaaaataaga gttgatatta aagatgttga agaatatatc
     5821 aacatacaaa aaactggtaa agtaaaagat acacatttta acaataaaaa tatagatata
     5881 aataataatt ttaaaattat tatctctggt caagatgtaa tattagacat tttagctagg
     5941 tctgttgaaa aaaaattgga tggaataagt acatttcgct cttatatagg tagttataat
     6001 gctttgtatg agttatataa tggcagagtt tccatagcat catcccatct atgggatttc
     6061 gaaacggatg aatataatag cacttttgtt aaaaaactat tgccagggat accatgtgta
     6121 cttataaatc tagcttatcg aatgcaaggt ttctatgttg ctaaaggaaa tcctaaagaa
     6181 ataaaaacct gggatgattt aacaaaatca aacataacca ttgtaaatag agaaaaaggt
     6241 tctggaacta gggttctact agatggtaag cttaggcttc taaactttaa tggtaaacat
     6301 ataaaaggtt atgataatga agagttatca catcttggag ttgcaagtac tgtttctcgt
     6361 ggaatagctg atgttggttt gggaaatgaa aaagctgctt tacaagtaaa taatatcgac
     6421 tttattcctc ttcacaagga gcgttatgat ttagttatta aaaaagagga cttacaaaat
     6481 ccagtttacc aaactataat taacataata aactctcctg agtttaaagc tgaacttcaa
     6541 ggcattggtg gatatgattt aaccgatact ggaaaaacta tagcaaaagt ttaaaattaa
     6601 aatcgcaatc tattttataa gatttataat ttttcacaaa atttataact taaacacgaa
     6661 ataaattgcg atttttagta tctcaaaaaa tttatatctt tcattttatc atctaatata
     6721 aaatttaata taattaattg tgtaagtata ttatattaat atatatttac ataatagtat
     6781 ttttccttaa tatagattaa catatgatat gttttagaca tcaatccctt tcctttctta
     6841 tatatatatg gtaacacata tgagtttaat agattatata caatattgct ttcttcttgt
     6901 attttttcaa ctaacaagtt atttataaaa ttatcttttt cgctctcaat aatatcactc
     6961 caatttatct taacttttaa tatatatctt cccactaggt atacacactc tacaataaat
     7021 aataaaaata caatacagtc agcacctgtt gtcttatata aatttcccga tactaatggc
     7081 ttagcgaaga aattataaaa attcaaagta tctcctgtat aatcaaaaag tattagtagt
     7141 agtcctagga ctctcaatac cttaatatta aacatatttt tatctttatt atttttattt
     7201 ttcatatata ctacctctct tttctttaga ttttattgta atctaatact aactctttta
     7261 tttgaggtat aaatgaattt aattggtaat tattttcatt tttactagaa attaattcac
     7321 attaataaag aaaacttata tttaaacata aaaatagcct atctattttt aataaataca
     7381 aaaataaata agctactaat attcaactaa cttttatcac ttaaaacatt actatctata
     7441 aatttaaata ttttattgaa gtatactgta ctatcatgct caaatccatc aacatgacca
     7501 ataccttcta taaaccatag ttctttttta tcatgtttaa ctaaatcata cattttttta
     7561 ctattttgat aattacaaac agtatctttt gtcccatgta taaacagtat aggtacatta
     7621 gatttctcga ctgactcata tggctttaca tcactgtacc aaaatccaga tttaaactta
     7681 ttatataaat ctccacaagt tactacataa tcgattaata tacctggtat tcgtttttct
     7741 agtactccca ttctaattgc atctttcatt tcagaatacg gagaatccaa tatataaaaa
     7801 tcgaccttct cattttttga atttatctct gtatgcatag cagctgttcc tgcccccatg
     7861 gaaaatccat gcactcctaa tcttccttca ggaaatttat tttttacaaa tttaactaga
     7921 ctatctaaat catacctttc ataaagacca aatgtgtaat tatctccgcc actattacct
     7981 gtatttcttt gattataaac taatacatta taacctttat ctaaatatcc atatgctacc
     8041 tttaacattt catagtaact acttccaatt ccatgaacaa gtataatagt atctcttgtc
     8101 tttatatttg aagttatgaa aagactttca agcttgtaat tattagaact ttgtatcatt
     8161 aattcagctg tcttgtagtt ttttaattta tccagtggtt tatcttttct ctcagaaaat
     8221 atttttacta tatcttcgtt ttttattaat tgctctaagc caactgaact attgtacact
     8281 aatttgccaa atatataaga tagaattaat aggattaata gaaacacaat tgatataatt
     8341 ataaatttat ttcttaaact aatataattt ttaaaactat ttttcatatt tatt
//
LOCUS       DAMJVM010000131         8363 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.136, whole genome shotgun sequence.
ACCESSION   DAMJVM010000131 DAMJVM010000000
VERSION     DAMJVM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.136"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     regulatory      210..253
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            354..1322
                     /locus_tag="PC523_003222"
     CDS             354..1322
                     /locus_tag="PC523_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDF2476711.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLHNHEKVSIIALAPLTNIAKALIKDRKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNTIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDLIDSVEGVI"
     gene            1324..1887
                     /locus_tag="PC523_003223"
     CDS             1324..1887
                     /locus_tag="PC523_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2476712.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDLYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQIFTDYAD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            1895..2584
                     /locus_tag="PC523_003224"
     CDS             1895..2584
                     /locus_tag="PC523_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2476713.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLEVLKNVTGKYGVLEVINSGSCFELPKDTLEEIKLIIKEKNIKKLFLESHWSYR
                     NRLEEMREYFEIPVIFKIGVETFDNDFRNNILNKNANFKTPQDVKDYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSNIKRDEELVRWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            2711..3439
                     /locus_tag="PC523_003225"
     CDS             2711..3439
                     /locus_tag="PC523_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2476714.1"
                     /translation="MMFNRGKWNKLDYNELLEYMMSMQDIKHRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISKGNWQEFLEVAEDTYYEEVMLQGMIIGNINSNFEETLYYVKRFI
                     PKINNWSVCDGFCSGLKSMKKYKENMYDILKKYVYSENPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKYEEETFLYIKNNNLDDFTYNKTLQKI
                     IESNRVDLEKKNIIRSMKRKTRRY"
     gene            3453..4127
                     /locus_tag="PC523_003226"
     CDS             3453..4127
                     /locus_tag="PC523_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2476715.1"
                     /translation="MNNNEERIDKFLELQSKGFRFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNGDLEEEKVKEYEKSKKNTISNRKKVSTKKKVSNEKVL
                     SKSIEQIEFNDTGLKNAKPKKNVKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNMKP
                     KKVSNKKDIHIDDETIDELKTTRIRIDKEVWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            4153..4989
                     /locus_tag="PC523_003227"
     CDS             4153..4989
                     /locus_tag="PC523_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476716.1"
                     /translation="MNKIVKKSISLMIILTIFIFTLTACEKDEQPDSVDIQVEDKKEI
                     KIDEGNAKVLNIGKSEIINMDEGDKVDTSTKIENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKSVEVYAYEYEAEGKIVYVNLKENTIDIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEEITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            5005..5676
                     /locus_tag="PC523_003228"
     CDS             5005..5676
                     /locus_tag="PC523_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2476717.1"
                     /translation="MKVLIVEDNKILLESVAEELRKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            5666..6898
                     /locus_tag="PC523_003229"
     CDS             5666..6898
                     /locus_tag="PC523_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2476718.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDAGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKINLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIVLELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSVEINVT
                     SDVDIGTTFELSIPKKLK"
     gene            7054..7371
                     /gene="trxA"
                     /locus_tag="PC523_003230"
     CDS             7054..7371
                     /gene="trxA"
                     /locus_tag="PC523_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF2476719.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            7388..8266
                     /locus_tag="PC523_003231"
     CDS             7388..8266
                     /locus_tag="PC523_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2476720.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SVDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKEKHIEVDG
                     VFVIKDSTSPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
BASE COUNT         3475 a          779 c         1465 g         2644 t
ORIGIN      
        1 atatttaaga tattattgta taataataat cggtttaaat gcgccacatt ttattaaagt
       61 tggttaataa ttttaaataa actttaataa aatacattaa aatataataa attcttctat
      121 gctataaaat ttattttata gctattaaat cggatgttta aaaatttttt aagagtctat
      181 agtaaggaat ctaaaattat aattttacat ctgtacttgt tcgagaactc caagtataaa
      241 aaactaagga attatatagt ggcagtatat aattaatacc cttagacaca cctttttgaa
      301 agtggaaaaa agatgtgtag ttctcctcga ccacaaatca aggaggataa aaaatggaaa
      361 aaagaaaagt aataatcgat tgtgacccag gaattgatga ttctttggca attcttctgg
      421 ctttaaactc accagagcta gaagtaattg gaattaccac gtgctgtgga aatgttccag
      481 caaatatagg tgcagaaaat gcactaaaaa cacttcaaat gtgttcttca ctaaatattc
      541 cagtatatat aggagaagaa gcaccactaa aaagaaaact tgtaacagct caagatacac
      601 atggagaaga tggtattgga gaaaactttt atcaaaaagt tgtaggagct aaagcaaaaa
      661 atggagcagt ggattttata ataaatactt tacataatca tgaaaaagta tcaataatag
      721 cacttgcacc acttacaaac atagctaaag cacttattaa agataggaaa gcatttgaaa
      781 atctcgatga gtttgtatct atgggaggag catttaggat tcatggaaat tgctctccag
      841 tagcagagtt taactattgg gtagacccac atggagcaga ttatgtttac aagaatttat
      901 ctaaaaaaat ccatatggta ggtttagatg taaccagaaa aattgtactt actcctaata
      961 ctattgagtt tataaataga cttgataaga aaatggcaaa gtatataact gaaataacta
     1021 gattctatat tgatttccat tgggaacagg agggaataat tggctgtgtg ataaatgacc
     1081 ctttagcagt agcgtatttt atagatagaa gtatatgtaa aggatttgaa tcatatgtag
     1141 aagttgtaga agacgggata gctatgggtc agtctatagt agattctttc aatttctata
     1201 aaaaaaatcc taatgcaatt gttctaaatg aagttgatga gaagaagttt atgtacatgt
     1261 ttttaaagag gctttttaaa ggttatgaag accttataga ctctgtggaa ggagtgatat
     1321 agtgtgaaaa gaaaaatatg tgtaaaaact attacactta tagcttttgg tatttccata
     1381 aatataatag gcgcttttat agctatggga ttaagattac ctgtatattt agattcaata
     1441 ggaactataa tgatagcttc acttttagga cctaaatacg ctgttgttac aggtgtgttt
     1501 ggaagtttaa taagtggaat tacgtttgat ttgtattctc tatattttgc accagttcaa
     1561 atatcaacag gattacttgc tggaatagca tttaaaaaga attttttaag gggtttaaaa
     1621 actccactag gagtactctt atttgccatt ccaacgtcta taataagttc aataatttca
     1681 gcatttttat ttggaggtat gacatcttcg ggttcatcct atatagtaca aatcttgaaa
     1741 gtattaggat tgggagatgt ttttagtgtg tttgtaaccc aaatatttac tgattatgca
     1801 gataagttgt tagcagttgt tttagtgaat ttaggactaa atgcagttcc aaaaacttta
     1861 aaggtgtcat tgactggagg aaaatagata taaaatggat agatatagtg aaataacaaa
     1921 taaaaatcaa agagaaattg ttctgcttaa aggatttcct tgtatatggg gaaagtgttc
     1981 tttttgtgat tatatagatg ataattctaa tttagaagaa gagatgaata aattaaatct
     2041 agaggtttta aaaaatgtaa ctggaaaata tggagttctt gaggtcataa attctggaag
     2101 ttgttttgaa ttacctaagg atactttaga agaaataaag cttattataa aagagaagaa
     2161 tataaaaaaa ttatttttag aaagtcattg gtcttacaga aataggcttg aggaaatgag
     2221 agagtatttt gaaattcctg tgatatttaa aattggagtt gaaacctttg acaatgactt
     2281 tagaaataat attttaaata aaaatgcaaa ttttaagact ccacaagatg taaaagacta
     2341 ttttgattca ccttgtatta tggttggaat aaaaggacaa acaaaagaga tgatagataa
     2401 agatatagag attatcttaa atacttttga aaaagcgaca gtgaatgttt ttataaataa
     2461 ttcttctaat ataaaaagag atgaagagtt agtgagatgg tttgttagta agtataaatt
     2521 cttggatgaa aatccaaata tagaagtttt atacaataat acagactttg gtgtaggaga
     2581 ttagcattaa tttagtttaa atttaaaatt gaaaaatttc ttatgttttt gaaaaagctt
     2641 ctaatagtta gaagcttttt ttgtagagtt ttatgttaca ataagaaaaa aggaattaag
     2701 gagcaaataa atgatgttta ataggggaaa atggaataaa ttggattata atgaactttt
     2761 agaatatatg atgtctatgc aagatataaa acatagagat tttaataaaa agcttatacc
     2821 tggaacagaa aatataatag gtattagagt tcctaatttg agaaagttat caaaagaaat
     2881 aagcaaaggg aattggcagg aatttttaga agtagcagag gatacatact atgaagaagt
     2941 tatgctacaa gggatgataa ttggcaatat aaatagtaat tttgaagaaa ctttatacta
     3001 tgtaaaaaga tttataccta aaattaataa ttggtcagtt tgtgatggat tttgttctgg
     3061 tttaaagtct atgaagaaat ataaagaaaa catgtatgat atacttaaaa aatatgttta
     3121 ttcagagaat ccatgggaaa ttagatttgc attagttatg tttttaatat attatgttga
     3181 tgataaacat attaaagaaa tatttgaata ctgtaataat atacaaagtg aagaatatta
     3241 tgttaaaatg ggtatggcat ggttgctatc aatttgtttt attaaatatg aagaagaaac
     3301 ttttctttat ataaaaaata acaatttaga tgattttact tataataaaa cgctacaaaa
     3361 aataattgaa tcaaatagag tagatttaga aaagaagaat ataataagaa gtatgaaaag
     3421 aaagacaaga agatattgac ggaggaatat taatgaataa caatgaagaa agaattgaca
     3481 aatttttaga acttcaaagc aaaggcttta gatttgcaga gatagctcaa aatctaaaga
     3541 ttagtcaaag tactttaaga acttttttaa ataaaaaagg gtataagtca gaaaaaggta
     3601 aatacatatt aaaagacgaa ttaaattata ataaaagttt agataatgga gacttggaag
     3661 aagaaaaagt taaagagtat gaaaaaagta aaaagaatac tataagtaat agaaaaaaag
     3721 taagcaccaa aaaaaaggtt tctaatgaaa aggttttaag taaaagtatt gaacaaattg
     3781 agtttaatga tacaggctta aaaaatgcaa agccaaaaaa aaatgttaag cctaagaaag
     3841 atagaaaaat aaatataact caagaagatt tagataagct ttgtgaagta tatgattggt
     3901 acttagaagt aaaagattac aatatgaagc ctaaaaaagt atctaataaa aaagatatac
     3961 atatagatga tgaaaccata gatgaattaa aaacaacaag aatacgtata gataaggaag
     4021 tatggaatga ttttgaaaga ttatgtagta attcaaattt tgaaaaacat caaatactta
     4081 cacaagcatt gaaagaattt atgaaaaaat ataaaaatct actataaaat actactatat
     4141 gaggtgctta atatgaataa aatagtaaaa aaatcaattt ctttaatgat tatattaaca
     4201 atatttattt ttacgttgac agcatgtgaa aaagatgaac aacctgattc agttgatatt
     4261 caagtagaag ataaaaaaga gataaaaata gatgagggaa atgcaaaagt attgaatata
     4321 ggaaaatcag aaattataaa tatggatgaa ggtgataaag tagatacatc cacaaagata
     4381 gaaaataact caacatttaa tatatccaat gttgaattga tatataatga atatgatgag
     4441 aataaaaaaa ttatatcttc agatagtaag gctttgttgg acatgacttt aatgcctgga
     4501 aaagttgctt atatagaatg tgggcataaa acctttgtaa agagtgtaga agtttatgca
     4561 tatgaatatg aggcagaggg taaaattgtg tatgttaatt taaaagaaaa tactattgat
     4621 ataagaaata ataatattaa gctagaaaat agtagtcagt atgaggtact atctacatca
     4681 gaacttaaaa aagtaaatga atcaaatgag gaaattacgt atcaagtaaa agtaaaaaat
     4741 tcatcatcaa aagatttagg aaatattatt cttaagacag cagaagttaa tgaaaatgga
     4801 gaatatttaa cagtaaatcg tgtaccatca tataagattt tgaagccttc agaagagaca
     4861 gatatagata tattgtgctc cacaaaggca aaaagtgttg aaatagtagg atatacatat
     4921 gatgatataa aggaaaaagc caatgtggat attgacttaa aatctcataa agttaagata
     4981 gacaaataaa ggtaggggat acttatgaag gttttaatag ttgaagacaa taagatactt
     5041 ctagaaagtg tagcagaaga gcttagaaag cattttgaaa ctgaaaagtg tgaagatgga
     5101 gaagaagctt tatacttagt aaatcaaaat atatatgatt tagttatttt agacttaatg
     5161 cttcctaata taaatggatt tgatatccta aagaaaatga gagttaataa tatagataca
     5221 cctgtactta tattgacagc aaaagaaaca ttagatgaca aagtggaagc atttactata
     5281 ggagctaatg attatttaac taaacctttt tatatggaag agttagtagc aagggtgtat
     5341 gctatattaa ggactaatgg aaagataaaa gaaagaaacg gattagaatt taaatcttta
     5401 tatttagata cactagaaaa aagagtgtat attgaaaaag aagaaataaa acttcaaaac
     5461 aaacagttta acttgcttga gtattttgta ctaaataaag gttcaatttt attaaaagag
     5521 cagatttatg atagaatatg gggaattgat tcagatgcaa ctattgaaat agtggaagta
     5581 tatgtaagta atctaagaaa aaagctcagt aaatatggat atgataaata tattaaaact
     5641 aaacgtaagg taggatatat attcgatgat aaataagaat gtatttacta gtactaaaaa
     5701 tcatctaata aaaatgtata ttattgtagt aggtagtttt ttaataatct tttctatatt
     5761 tatatattca tattttaggg gacttacata tagtggaatt gatagtgaga taaatgatga
     5821 gcttgaatat atagtttctc aatttaaaag aacttcattt ttgaacccaa taagactcaa
     5881 agaccctaaa gatatggtgt atgtatatga agatggaagg ataagttact atactcaaaa
     5941 tgaatatttt gatgagttgc ttccagatag aagactagat aaaaaaaatt cgttttttaa
     6001 atacacagaa aatggatata ctttcagaga attaaatgta gatgcaggta gataccaaat
     6061 acagataatt agaaatatag attctgagat gaattcttta agacagctta catctgttct
     6121 tataattgga atattaattt ctgtaataat tacttacttt gtagctgtat atctcactag
     6181 aaaagcttta attccaatag aaacagcatg gaaaaatcaa gctaagttta tacaagatgc
     6241 ttctcatgaa ttaagaacac caataacaat agtttcatca aaattagaaa gtatgttaaa
     6301 atccccagaa agtacagtaa atgatgaagt tgaaacaata gctactgcaa tgaaggaaac
     6361 taggagactt aaaaaaatga taactgattt attgtctctt actaaagagg attctattgt
     6421 taaaattaat ttagaagaaa tagacttaga aaagttattg gaagaaatat cagaagacta
     6481 tattgacata gctgaatttc aggaaaaaag attcgtattt aattctaaac tgaagaataa
     6541 agttataata acagataaaa ataaattaag acagcttata ttgattttta ttgataatgc
     6601 ctttaaatat acaaaattgg gagatgagat agtattagaa ttaaaagaag atatagaaga
     6661 tgaggtaact ttgcttataa gtgatactgg tataggtata aaaaaagaag aaatacctct
     6721 tatatttgat agatttttta gaagtgaaaa tgtaagaaat aaagatttag aaggttctgg
     6781 aataggactt tcaatagctc gaatgattag cttaaacttg tctgttgaga taaatgtaac
     6841 ttctgatgta gatattggaa ctacatttga attatcaata ccaaaaaagt taaaataata
     6901 aaaatataaa aagttgtttt tagaaataaa gaatgggtat actaattagt ataaaataaa
     6961 aaataaaaag accttataaa agtaaaatta tatttataaa tttcatagta taattatata
     7021 gggataataa agtttaatag gaggatttaa aaaatggcaa aagttataaa tacatctgaa
     7081 tttagaagta gtgttgaagg tagtaaggga gtagttgtag ttgatttctt tgcaacttgg
     7141 tgtggacctt gtaacatgtt gggacctgta tttgcagaat taggtgaaga gatgaaagat
     7201 aaagctagat ttgtaaaagt tgatatagat gagagcttag aaatagctca acaattcaat
     7261 gtgtctacag taccaactat gattatattt aaagatggaa aaccagtaga aactttaatt
     7321 ggattcatgc caaagaacaa aattgaaatg caagtaaaat catatttata aaaaatgggt
     7381 gagtattatg agatatgata tagctataat tggtagtggt ccagctggat tgtctgctgc
     7441 tataaatgca aaaataagaa ataaaacaat tatcatgttt ggtaatgata atttaagtaa
     7501 taagcttgtt aaagcccctt cagtagataa ttatttaggt ttttatgata taagtggtga
     7561 tgaattaaaa gataagttta aatcacatat agatagtatg gatatttcaa ttgaaaataa
     7621 aagaataaat aatatttatg ctatgggaga atactttaca ataatgtctg gaaatgatat
     7681 gtatgaggct actactgtaa tccttgcaac tggagtagag tatactagac caatcaaggg
     7741 tgaagaagaa tttctaggaa gaggtgtagg ctattgtgct acatgtgatg caccacttta
     7801 tagaaataaa aaagtagcag ttataggata taatgaagaa tctaaagaag aagcaaattt
     7861 tttaagtgaa ctcacttcaa agacttattt tatacctatg tacaaaaaag ataatcttat
     7921 gagaagttca gataacctag atgattctat tgaagtcata catgacagac cagtacaaat
     7981 agatggagat aaacttgtaa acaaagtatc ttttaaagaa aaacacattg aagttgatgg
     8041 agtattcgtt ataaaagata gtacatctcc aagtgccttg gttcctggaa tagaaattga
     8101 tggcattcat attaaagttg acaataatat gaaaactagt atagatggat gttttgctgc
     8161 tggagattgt gttggaaaac catattctta tataaaagca gctggccaag ggcaaatagc
     8221 agctttaaat gctgtttact atttagataa attaaagagg gcttaatatt tatattgatt
     8281 aactttttaa agatatttat aaaaaactgt cttgaaataa ttttattatt tcaagacagt
     8341 tttttatttg taaattacta tta
//
LOCUS       DAMJVM010000132         7571 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.137, whole genome shotgun sequence.
ACCESSION   DAMJVM010000132 DAMJVM010000000
VERSION     DAMJVM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7571
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.137"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            199..1176
                     /locus_tag="PC523_003232"
     CDS             199..1176
                     /locus_tag="PC523_003232"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDF2476721.1"
                     /translation="MRDKIVEIMLNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNICYKLDTEFIGKNIEHLETIDSTNEHAKRIAVN
                     SLDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNMGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IAEKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNKELVKCLDINEEGNLLVQRSDGNIKEIISGEVSIRGEKGYV"
     gene            1169..2128
                     /locus_tag="PC523_003233"
     CDS             1169..2128
                     /locus_tag="PC523_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HDF2476722.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMIQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGNFKCRPDLKMGYRACEVANIDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLVVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKAIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKK"
     gene            2322..2912
                     /locus_tag="PC523_003234"
     CDS             2322..2912
                     /locus_tag="PC523_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2476723.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGGAIGFIFGISSLLNAIINPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            2931..3701
                     /locus_tag="PC523_003235"
     CDS             2931..3701
                     /locus_tag="PC523_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HDF2476724.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIINMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            3838..4806
                     /gene="dusB"
                     /locus_tag="PC523_003236"
     CDS             3838..4806
                     /gene="dusB"
                     /locus_tag="PC523_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDF2476725.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEDEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            4915..5394
                     /gene="greA"
                     /locus_tag="PC523_003237"
     CDS             4915..5394
                     /gene="greA"
                     /locus_tag="PC523_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDF2476726.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            5409..6938
                     /gene="lysS"
                     /locus_tag="PC523_003238"
     CDS             5409..6938
                     /gene="lysS"
                     /locus_tag="PC523_003238"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDF2476727.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMEIKDNFESLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNAEDPRRTDRFEVFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            7247..7441
                     /locus_tag="PC523_003239"
     CDS             7247..7441
                     /locus_tag="PC523_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476728.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFENTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
BASE COUNT         3038 a          757 c         1510 g         2266 t
ORIGIN      
        1 actttcagtt ttttattatt ttaaaattat ttaaataaaa tataaaatca cataaatcat
       61 caatataaaa tataaaacaa ctattataat aaatgaaaag gaaatttaaa attaatagta
      121 ttaaaagtat attaataatg tataattatt tattagaaga aaataagtca tgaaatcatg
      181 tcatattaga ggtgaaatat gcgagataaa atagtagaga ttatgttaaa taataaatct
      241 gaatttattt caggagaaga attatcaaaa caattagggg tgtcacgtgc agctatatgg
      301 aaacatatga aagccttaaa ggaagaggga tataatatag aatctgtaaa taaaaaaggg
      361 tatcgattag cggaaaatcc aactgattta ttaagcccac agaatatttg ctataaatta
      421 gatacagagt ttattggaaa aaatattgaa catcttgaaa ctatagattc aacaaatgaa
      481 catgctaaaa ggatagctgt aaatagttta gatggaactg taattttaag cgaacatcaa
      541 gatatgggga agggacgatt gggaagaaga tgggattcaa aacctcatga aggaatctgg
      601 atgagtatta tattaaaacc agatatggaa ccattcaaag ctccatttat aactttaata
      661 gcaggagcaa gtgtaacgaa ggctttaaat aacatgggag caaatacatt aataaaatgg
      721 ccaaatgatg ttattttaaa tggaaaaaaa atatgtggta tattaacaga gttatctgct
      781 gaaattgaaa gaacaaacta cgttgttctt ggaattggaa taaatgttaa aataatggat
      841 tttgaacaag aaatagctga aaaagctaca tcattgtata aagaaggata catgttatcc
      901 agagtagata ttgttaggaa tgtattgact gaatttgaaa aattatattt agattatact
      961 gtaaataatt ccaaagaaaa aaccttaaaa atatgtagag attattctgc aattatagac
     1021 aaaaatatat atgtattaaa aggagataat aaagaactag tgaaatgttt agatataaat
     1081 gaagagggaa atttattagt tcaaagaagt gatggaaata taaaagaaat tatttcaggt
     1141 gaagtttcta taagaggaga aaagggatat gtataataat attttggata tagaaggttt
     1201 aaaggttggg caagttgagg atagaaatgg tcttactggg tgtacagtag taatatgtga
     1261 agaaggagct acttgtggtg ttgatgtaag aggttctgct ccagggacta gagaaaccga
     1321 tttattagac cctgtaaata tgattcaaaa agttcatgca gtagttttgg ctggaggttc
     1381 tgcatatggt cttgaatcta cttgtggtgt aagtagatac ttagaagaaa aaggaattgg
     1441 ctttgatgta ggtgtaggta aagtaccaat agttgtagga gcagttctat ttgatttagg
     1501 agtaggtaat tttaaatgta gaccagattt aaagatgggt tatagagcat gtgaagttgc
     1561 aaatattgac attttaaatc aaggaaatta tggagctggt tgtggagcga ctgttggaaa
     1621 gataagaggt caagaatatg ctatgaaagg tggcataggt agttattcta taaaattaga
     1681 taatggtcta gtcgtatctg cattaattgc tgtaaatgcc cttggagatg tttatgaaaa
     1741 tggtaaggtt atagcgggtg ctttaaatga taaaaagaca gaaacaattg atacatataa
     1801 attaatgaaa caaggtgaaa gtaaaggtgg atttagtgta gataatacta ctattgggat
     1861 aattgcaaca aatgctaagt taactaaagc tggatgtaaa aagatatctc aaatgggaca
     1921 tgatggatat gctaaagcta tttttcctat ccatacacct catgatgggg atacaatatt
     1981 cacaatatca acaggagaaa tagaagcaga tataacatta gtaggttcat tggctgtaga
     2041 ggttgtggaa aaaagcataa taaatgcagt aaaaaaagcg gatagtgtag aaaatataat
     2101 ggcatataaa gatatagtaa aaaaatgaga aatatgatga aattagcata tttttcttgt
     2161 caaattgttg acaatggcaa ctcatcttga taaaattatt cttgttagag cgggcatcct
     2221 acaatggtag cttgactcat aattataaga aaaaaacgaa aaaaatttaa aaaattaagt
     2281 agtccggcat cgtaatgagc tggaacggag gtgtattttt tatgatgaac acaagtgcat
     2341 cagtaagaaa agtaaatgta agaaagatga ctataatagg agtactatca gctatttcta
     2401 ttatgctatc tatgactcca ttgggattta ttccagttgg acctacaaaa gcaactataa
     2461 tgcacatacc agttattatt ggagctatta tggaaggacc aatagttggg ggagcaatag
     2521 gatttatatt tgggatatca agtctcttaa atgctataat aaacccaaca gtgacatctt
     2581 ttgtttttat aaatccatta gtttcaatat taccaagagt tatgataggt gtattggctt
     2641 attatgtata tcagttaata attaaggcta caaacaaggt atacatatca ggtttaatta
     2701 caggggctat aggttctctg ttaaatacag ctggagtttt aggaatgata tatgtcttat
     2761 atgctgacaa gtatcttcat gcaatgggta aaagtggttc agcaggaaaa gtaataatgg
     2821 ctcttgccgc agcaaatgga gtgccagaag caatagtagg ggctttagta gtagctgctg
     2881 ttgccactgt acttaagaaa agcaaaaaat agaaagaagg ttaatgacat atgcttctag
     2941 tatttgatgt tggaaatact aatatggttt taggtatata taaaggtgac aaattagtta
     3001 attactggag aattaaaaca gatagagaaa aaacttctga tgaatatgga atcttgataa
     3061 gtaatctatt tgattatgat aatgtgaata taagtgatat tgatgatgtt ataatatcat
     3121 ctgtagttcc aaatgttatg cattctcttg aaaacttttg tataaagtac tgtaaaaaac
     3181 agccattaat agtaggtcca ggcataaaaa caggtctaaa tataaaatat gataatccaa
     3241 aacaagttgg ggcagacaga atagttaatg ctgtagcagg tatagaaaag tatggagcac
     3301 caagtatact tgttgatttt ggaacagcaa ctacattttg tgctatctct gaaaaaggtg
     3361 aatatttagg tggaacaata gcaccaggaa taaaaatatc tagtgaggct ttatttcaaa
     3421 gtgcgtctaa actacctaga gtagaattag ctaagccagg tatgactatt tgtaagagta
     3481 ctgtatcagc tatgcaatct ggaataattt atggatatgt tggtttagtt gataaaataa
     3541 taaatatgat gaagaaagaa ttgaattgtg atgatgttaa ggttatagct acaggtggat
     3601 tagctaaact gattgcttca gagacgaaaa gtatagatta tgtagatggt tttttaacac
     3661 tagaaggatt gagaataata tatgaaaaaa accaagaata attaatatac agggaattgg
     3721 gttatatatc caattccctt tttatgatag atttctacat atgtgtggta tattataatg
     3781 aggaaataag aatttaaagt taaattacaa aataaaggaa aaaataggag aaaaacaatg
     3841 aaaataggaa atttagaatt gaaaaataaa gtttttctat cacctatggc aggtgtaaca
     3901 gaccttccat ttagattaat ttgtaaagaa cagggatgtg ggcttttata tacagaaatg
     3961 ataaatggta aggcactttg ctatgatgat gaaaatacta aaaaaatgct taagatagaa
     4021 gatgaagaac atcctgtagc agttcagata ttcggttcag agccagagtt tatgggaaga
     4081 gcagcagaga taatgaatga ttattcaaat gagattctcg atataaatat gggatgtcca
     4141 gctccaaaag tagttaaaaa tggagatggt tcagcactta tgaaaaatcc taagcttgca
     4201 gaagaggtat tgagggcagt agtgaagaac tcaaaaaagc cagtgacttt aaagatacga
     4261 aaaggctggg atgacaatag tgtaaatgct gttgaaatag caaagatagc agaagattgt
     4321 ggaataagtg ctctagcaat acatggaaga actagagaac agttttatac aggtaaagca
     4381 gattgggata taattgcaga gattaagaaa aatttgagta tacctgttat aggaaatggt
     4441 gatgtattta caatagaaga ttcaataaat atgttagaca aaactggttg tgatgctata
     4501 atgataggta ggggtgctca aggtaaccct tggatattta aaagaataaa ccattatatg
     4561 aatacaggcg agattttgcc agagccaact cttaacgaaa aaataagtac tgccattaag
     4621 catttaaaac ttgcagtaga agaacatgga gagtatgtag ctgtaaggga aatgagaaaa
     4681 cacatcgctt ggtatttaaa aggacttaga aactctgcaa aattaaggga tgaaattaac
     4741 aaaatagaag actatcaaga agttgtgtcg aaattggagt actatatgga agactccttg
     4801 acataaaaat aatagtgact tataatatag cgcataaatt aaaaaaagat tataaaattt
     4861 ttaaaatata attaggaaaa ttcctaagga atttgtgaag gagttgtatt tataatggaa
     4921 gaaaataaag agtttttatt gacacaagag ggatatgata aattagaaga agaattagaa
     4981 aacttaaagg ttgtaaagag aaaagaagtt gcagagagaa taaaagttgc tatatctttt
     5041 ggtgatttat ctgaaaatgc tgagtatgat gaagctaaaa aggaacaagc acaagttgaa
     5101 gaaagaattt taaaattaga aaatatggtt agaaaagctg ttatcataga tgaaagcaaa
     5161 atagacctta atgttgttac aataggatca atagttaaag ttaaggattt agaatttgat
     5221 gaagaggttg aatatactat agttggttcg acagaagcag acccatatga tggtaaaata
     5281 tcaaacgaat ctcctgtagg aaaagcactt ttaggtagag ctgcgaaaga agttgtggaa
     5341 gtgcaagttc ctgatggagt tgctaagttt gaaatattag aaataagaag ataatttgga
     5401 ggaaaaaaat gaaaaataat caacaaagca atgaagaagc gcaaattcaa gaagacctta
     5461 gtgaagtact tcaagtaaga agagataaac taaaaaaatt acaagagtca ggtagagatc
     5521 cttttaaaga aagtagatat gatagaactc attattctat ggagataaaa gataattttg
     5581 aatctcttga aggcaaaact acaaaaatag caggacgtat aatgagcaag agaatacaag
     5641 ggaaagctgg ctttatagat attcaagacc aagagggaag aattcaatct tatgtaagac
     5701 tagatgctat aggagaggaa gaatatagtg tattttcaac atatgatatt ggagatatag
     5761 ttggaattga aggggaaatc tttaaaacta aaaaaggtga aatctctgta aaggctaaat
     5821 ccgtagtatt actatgtaaa tctttacaag ttctaccaga aaaatatcat ggattaaaag
     5881 accaagaact aagatataga caaagatatg ttgacttaat agtaaatcca gaagtaaaaa
     5941 atgctttttt aattagaaca aaagcattaa aagcattaag agcatactta gatgatagag
     6001 gatttttaga agttgagact ccaatattaa atactatagc aggtggagca aacgctagac
     6061 catttataac taatcataat actttacata taccaatgta tttaagaata gcaaatgaat
     6121 tgtatttaaa aagacttatt gttggtggat ttgataaagt ttatgaaatg ggaagaatgt
     6181 ttagaaatga aggtatggat ttaaaacata atcctgaata tactgcaata gaattatatc
     6241 aagcatatgc agactatact gatatgatgg aaataacaga aaatgttatt gcgcatatgg
     6301 ctgaagttgc tacaggaagt atgatagtta actatcaagg aacagaaata aactttacac
     6361 caccttggaa aagaatgagt atggaagatt gtgttaaaga atattctggt gtagattttt
     6421 ctactataaa tacagatgaa gaggcattag aagttgctag agaaaaaggt atagagataa
     6481 aacctggaat gagaagagga gaagtaataa atgctttctt tgaagaattt ggagaagata
     6541 aattaatcca accaacattt ataactcatc atccagttga agtatctcca ttatctaaga
     6601 gaaatgctga agacccaagg agaactgata gatttgaggt atttgctaat aagtgggagt
     6661 tagctaatgc attttctgaa cttaatgacc ctattgacca aaaaggaaga tttatagacc
     6721 aattgagaaa aagagaatta ggtgatgatg aagcttttga aatggatgaa gatttcttaa
     6781 aagcattaga ggtaggtcta cctcctacag gaggactagg aataggtata gatagagtaa
     6841 tcatgttatt aactaactct ccatctataa gagatgtatt attattccct actatgaaac
     6901 ctattgataa caatccaaat aaagaagaag agaactaaga aatattagca ttctactaag
     6961 ttttaaataa agtttagcta ttaaattagc aataaatagc taaactttat ttaaaatgtc
     7021 agtgtagaat gctttttata tgtttcaaag attcttttag atatttaatt tataaattac
     7081 caacaaacgt tagaaattga tattagtttt tgttgattga gactttatat ttagaaaata
     7141 tgattgaaag taatttatta tgaaaatatt tagaataatt tttgagaagg taaaaagaat
     7201 aatttctgag aatactatat ataaaaaatt attgggggaa tgtctgatgt ttcaagtgat
     7261 taatttttta tgtttattct gtgttggatt tttagttctt gatgttatga tatatttttg
     7321 taaggatgca atggatattt ttgagaacac aactaatgtt aataaagtta agagtataaa
     7381 tgtttgggct gatagagtca gagctaattc atattcttat gaaaataaaa ttgcaaaata
     7441 ggtaaaatta tatatgcatg tattgtttgt aatatcaagg gttatatcta ttggttttaa
     7501 ttaaaattat taagaaaaac aaaaagttat aaccttttta aaataaaaaa caaaaaaagt
     7561 attgacgaaa t
//
LOCUS       DAMJVM010000133         7554 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.138, whole genome shotgun sequence.
ACCESSION   DAMJVM010000133 DAMJVM010000000
VERSION     DAMJVM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7554
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.138"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(609..797)
                     /locus_tag="PC523_003240"
     CDS             complement(609..797)
                     /locus_tag="PC523_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2476729.1"
                     /translation="MTLQDRIKEERLKLGLNQVELSKKFNMTKQTVSNWESENRIQDT
                     LMLTKLADFLCISRFSLG"
     gene            985..1218
                     /locus_tag="PC523_003241"
     CDS             985..1218
                     /locus_tag="PC523_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476730.1"
                     /translation="MNTKKTKGIYKNGGLKVSRTAICSVCGELLVDGYGDEFTNTKII
                     DLHGREFVIIIYKNCLEELLRKCANGGISNDNN"
     gene            1205..1462
                     /locus_tag="PC523_003242"
     CDS             1205..1462
                     /locus_tag="PC523_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476731.1"
                     /translation="MIIIRSQDRLDLMRVNRVEIDSKYVYAVFGEESNVREIGRYKSE
                     ERAIEVLSRIQDAIIAGTKFDIIKKDGVRYYKEKVFEMPVE"
     gene            1455..1835
                     /locus_tag="PC523_003243"
     CDS             1455..1835
                     /locus_tag="PC523_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476732.1"
                     /translation="MNKEGSEMLKVEKYFSGSLGINIIEDDPTCRNYLALYCCLVGIE
                     RKGKKVIPRIDSMLSEFGVKSRCKRPHNNKLIRLINVKTGEVKEFKSIDSTACFLGLK
                     QPAVSHILKRKTPSRSGWRAEYIK"
     gene            2372..2566
                     /locus_tag="PC523_003244"
     CDS             2372..2566
                     /locus_tag="PC523_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476733.1"
                     /translation="MNILIYKYIVNEHRDEADELDRDYGEFKIFNGGKCFSGCGRNFY
                     TLAKFKKMIITCPHYEKNIK"
     gene            3552..4124
                     /locus_tag="PC523_003245"
     CDS             3552..4124
                     /locus_tag="PC523_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476734.1"
                     /translation="MNENLSKEIKILGTIEVEEMKFHDIEGGFGVGKKSMSVKDIDEI
                     HNGKLKDINKNINNNRKRFKDMINTLDLKVSDLKSLSLEMGYSNQSYANANNIYLLPE
                     RDYSKRLKILEGKQYEKIVDGYFSMRKELNNPLLSASKELQAIFMLDKKQEFLETKIE
                     SVNEKLENFMDDAPLFNIEFESIVKIVLWK"
     gene            4567..4938
                     /locus_tag="PC523_003246"
     CDS             4567..4938
                     /locus_tag="PC523_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476735.1"
                     /translation="MKKQIMALGLTGLIGLGGLGLLANKSFADTPNKLKGDTTVAQGL
                     NKNATAKDVTEQVDTTIGKGLDKNAIVKDVVENNGEKSDTTVAPGLDKNATVKDTAEK
                     GNTTIAPGLDKNTIVKDIVVK"
     gene            5077..5334
                     /locus_tag="PC523_003247"
     CDS             5077..5334
                     /locus_tag="PC523_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476736.1"
                     /translation="MVKCTKLIGGVITMKKNILGVLLPLLTMIIIFVLSFENIITNSL
                     GLSDMDIKGILISGVVLYIPVSFLVNGIICAKKQIKWKISK"
     gene            5657..5941
                     /locus_tag="PC523_003248"
     CDS             5657..5941
                     /locus_tag="PC523_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476737.1"
                     /translation="MTFEVEFYTFEGINMEAEMYVQVAMMASKVVKITGVDEVIKKLV
                     KKFLNNKVEEALGKQRSKKELKLIENNFNEYMERSYKNYIYMNTIVFRNQ"
     gene            complement(6218..6454)
                     /locus_tag="PC523_003249"
     CDS             complement(6218..6454)
                     /locus_tag="PC523_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476738.1"
                     /translation="MAPNFNVENIVLEDKEIVSTPQKYVLISRYYDNGKTIAFIEMNS
                     TKYKTEYIRNFDQYVDEFSTLEETEECLQECLSA"
     gene            6791..7018
                     /locus_tag="PC523_003250"
     CDS             6791..7018
                     /locus_tag="PC523_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2476739.1"
                     /translation="MAMVSISFGEKIKIILKRKNMTIGELAEKTGQTRQNLSNKFSRD
                     NFSEKEIRKFAKILECEFDFYFIIKDTNEKV"
     gene            7005..7343
                     /locus_tag="PC523_003251"
     CDS             7005..7343
                     /locus_tag="PC523_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476740.1"
                     /translation="MKKYKIGGNFYMEVTLHTGEINFFKEHEVAKNYIGELYNFIEEY
                     KLSMETVSILTNIDIKKLNDFYIGKGTLHYDELVLINSVIIMPFIDVFKTAKHNYEMI
                     IKSSKKYEED"
BASE COUNT         3130 a          690 c         1213 g         2521 t
ORIGIN      
        1 aaaaaatata attaaaaaaa cgattaataa tctataaaaa tggaaaaaac atccaatatc
       61 ctacaaaaat agattttttt attacaatta caagttttta ttgcatctat ttagcaaaag
      121 tattattatg caagtaagat aattatcaaa gacaattctg aatagtctga tttaaaagat
      181 acaattaaag gatataaata ggaataattt ataaatatta cagaagattt ataactaaaa
      241 ttacgaacgt aagtttgtta tatggggtag ttgtgtggat gaatcaaata aattaatatt
      301 agaattgaat aatattttct gtgaattaga gagtattaat aaatataagt ttaatgaata
      361 taaagaaatt gttaaaaact tacacaacaa aaaagaagag gaataatttc tcttctttta
      421 aataaaatta tttttgatta tcaatctttg atttttcttc ctctataaag acatgagcaa
      481 tttttaacat cttatcttta gttttatcat ctaaatcaag aaatatcttt attaattctt
      541 ttacatcgtc ataactatct agttcatcaa tgaaattttc ataattattt atttgctcat
      601 tattagcttt accccaaaga gaatctactg atacacaaaa agtcagctaa tttggtaagc
      661 attaaagtat cttgaattct attttcgctc tcccaattac ttacagtttg cttagtcatg
      721 ttaaattttt tagacaattc aacttgattt aagccaagtt taagcctttc ttcttttatt
      781 ctatcttgaa gagtcataat attgctcctt ttattgatgt ttatataagt aactataaac
      841 tatttgttat attttttata aaacatataa taaaataaat actttttcga aaaaaatgta
      901 aaatttgtgt tgatagtaaa ctaaaagtag actgatatat aaatataaag ttaacataaa
      961 gttgactaga aaagaggtgt taaggtgaat actaaaaaga ctaaaggcat atataaaaat
     1021 ggaggtttaa aagtgagtag aacagctata tgtagtgttt gtggggaact tctagttgac
     1081 ggttatggtg atgaatttac aaatacaaag atcatagatt tacatggtag agaatttgta
     1141 attattatat ataagaattg tttagaagaa ttattaagaa aatgtgctaa tggggggatt
     1201 agtaatgata ataattagaa gtcaagatag attagattta atgagagtta atagagttga
     1261 aatagatagt aaatatgtat atgcagtgtt tggagaggaa tcaaatgtta gagaaatagg
     1321 taggtataaa tcagaagaaa gagctattga ggtattaagt aggatacagg atgctatcat
     1381 tgcaggaact aagtttgaca ttataaaaaa agatggggtt agatattaca aagaaaaagt
     1441 gtttgaaatg ccagttgaat aaggaggggt ctgaaatgtt aaaggttgaa aaatatttta
     1501 gtggttcttt aggaataaat attatagagg atgaccctac atgtaggaac tatttagcat
     1561 tatattgttg tttagttgga attgaaagaa aaggtaaaaa agtaataccg agaattgata
     1621 gcatgttatc tgaatttggt gtgaaaagta ggtgtaaaag acctcataat aataagctaa
     1681 taaggttaat aaatgttaaa actggagaag taaaagagtt taaatctatt gatagtactg
     1741 cttgtttttt aggattaaaa caaccagcag ttagtcacat attaaaaaga aaaactccaa
     1801 gcagaagtgg ctggagggct gaatatatta agtaggaata atggaagttt caaggactga
     1861 atacacaatt aagagagcaa aagagttgta tggcaatggc aaggatatgt ttattgctat
     1921 agataattct agagaagaat atgaggagat tgttaaaaat gaatatttta actagtatta
     1981 cattaataat gactagtttt ataataggta gaatttatga atatagattg aatttaaaag
     2041 agtgtgaaaa ttgtaatagt aaaggctacg agaatatatg aaatttatgt agataaatag
     2101 taaaggcaat tttatgaatg aaactaaaaa ctttatagaa ttactttaat ttatataaag
     2161 gagtaattat taatatgatt acaacagaat ttatagttac ttggtagaca actagaaaac
     2221 gggaaaaagg aaagacatat agtcatgtat ttacagattt agagaaagca agagcattta
     2281 agaaagagga ggaaaatata aaaaaattat aatgtgaagc ttttaagtag agctatagtc
     2341 gaaatcgaaa tagattaggc aggtgttttt aatgaatatt ttgatatata aatatatagt
     2401 taatgaacat agagatgaag cagacgagtt agatagagac tatggagaat ttaaaatatt
     2461 taatggaggt aaatgtttta gtggatgtgg taggaatttc tatacattag ctaagtttaa
     2521 aaaaatgata attacttgcc cacattatga gaaaaatatc aaataaaact ttttaaaaat
     2581 ggaaaagtga atatgagagt aattgagtaa ataaaggaaa gtgattatat gaaaagaaga
     2641 agatgcagtt ggtgtgggaa attattttat cttaaggaaa aatctaagga ggtttattgt
     2701 tataaggaat gtaaaaagaa ggctaacagg gtaaagagct ttaatgacta aggaatataa
     2761 tttaaaacaa aataattaga aggtaaaaaa tatttaatta attatacttt ttaccttctt
     2821 aaaatagaaa aacttaacta ttaagatatt tgtgagggtt atgtatccaa aataataata
     2881 ttattgcaaa tagaggactg aaccaaaatg ccagtaaagt ataaagacta ctatttaaag
     2941 catttttagt cgaaactcta ttacaagcga ttaatagtat tatagataaa atattagcaa
     3001 taatttctaa tatatttgaa ttaaagtctt gaaaaaagac taaaaaagaa aaaatataag
     3061 agatactcat acatataaaa aaacaataat tcagtatttt aaattttaat agttgttttt
     3121 tattatcatt gattgccata agtactccta aaattataga tagtatgata aatgtatttg
     3181 atgcttgcaa ataagaaaag atagtcataa atttattctc ctaatattaa ctataaattg
     3241 taaaaaaata atttaaataa ctaacaattg aataaccaat aaaaaatgta aataaataaa
     3301 aaagaaactt tttaaatcca tttaatttaa atcttttttc tatcttttga caaatgaaat
     3361 ttatcggaat aaaaacaaaa gtcgcaatta aaaaagttat taaatacttt gacataggat
     3421 aatagtccat aaataatcac ctcataaatt agtataacca tagattaata tgagttgtta
     3481 ataatagcaa tcaatttttc agaactgtaa ataaattgaa ataaattatc caaaaaaaag
     3541 agcaatagac tatgaatgaa aatttaagta aagaaattaa aatactagga actatagaag
     3601 ttgaagaaat gaaattccat gacattgaag gtggatttgg agtaggtaag aaatcgatgt
     3661 cagtaaaaga tatagatgag atacataatg gaaaactgaa ggatataaat aaaaatataa
     3721 ataataatag aaaaagattt aaagatatga taaatacatt agatttaaaa gtgagtgatt
     3781 taaaatcact gagtttagaa atgggttact ctaatcaatc ttatgcaaat gcaaataata
     3841 tttacttatt acctgagaga gattattcaa aacgactaaa gatattagaa ggtaaacaat
     3901 acgagaaaat agttgatggg tatttctcta tgagaaaaga attaaataat cctcttttaa
     3961 gtgcatcaaa ggagttacag gctatattta tgttagataa gaaacaagaa ttcttagaaa
     4021 ctaaaataga aagtgttaat gagaaattag agaattttat ggatgatgca ccactattca
     4081 atattgagtt cgagagtatt gtaaaaatag tattgtggaa atgaaaattt ccctatcaaa
     4141 actaaataat tggctagggt taagggatta ccctagtcac tatgaacaga ctaggcaggg
     4201 cgtgaggacg ctattagttc aattctaact atgttcaaaa tcattgatac actatattaa
     4261 gtatctgaat tgagactaaa attcatacag ttttgtatct taattcagaa tctgaaaatt
     4321 gagtggggtt tggcaacctc actaaccatg cagatactgg tatttaatct aagttcgatt
     4381 cttaggacct gctacataat atgaatctcc ctctttataa aatgactaag ttcgggtaag
     4441 cttagtcttt tattttttaa acaagaaaat gagaatttgg attaattagc attgtaaaca
     4501 atcagtaaac attaatatga tattatttgt ttagaaaata aaacaaagaa aaaggagaga
     4561 ataattatga aaaaacaaat tatggcttta ggattaacag gattaattgg cttaggaggt
     4621 cttggattac ttgcaaataa atcatttgca gatactccta ataaacttaa aggagataca
     4681 acagtagctc aaggattaaa taaaaatgca actgctaaag atgttacaga acaagtagat
     4741 acaacaatag gtaaagggtt agataaaaat gcaattgtta aagatgttgt agaaaataat
     4801 ggagaaaaaa gtgatacaac agtagctcca ggtttggata aaaacgcaac tgttaaagat
     4861 actgcagaaa aaggcaatac aacaatagct ccaggattag ataaaaacac aattgttaaa
     4921 gatattgtag taaaataatt tagtttttat agctggcaaa agggtggaaa tttattctgc
     4981 ccttttgttt tattaataaa aatttacata tattaaatat ggatagcaaa gacttagatt
     5041 aatttctagc tcttttatat tttaaaggtt gtgtttatgg taaaatgtac taaactaatt
     5101 ggtggggtga taactatgaa gaaaaatatt ttaggagtat tgctaccatt actaacaatg
     5161 attataatat ttgttctatc atttgaaaat ataataacta atagtttagg attaagtgat
     5221 atggatataa aaggaatcct tatttcaggt gtagtattgt atataccagt ttcattttta
     5281 gttaatggta taatatgtgc taaaaagcaa ataaagtgga aaattagcaa ataaagtgga
     5341 aatttccagt tttaatatca tttttaggct cagttgttat tatgatgtat ctaagttttg
     5401 atagcattga aatggcattg tatttatcat attattttat atcatattat attggatatt
     5461 ggataactag tgcaatgaag aaataaaatt aaggtgtaaa tataaaaaat agaataagtt
     5521 gagagactat ctttataaat ggtctttttt tatacaatga atcaaaaatg aaatatattt
     5581 atgcattatg gagagaaata tggtaaaagt attatttgga attattttgt agttatagaa
     5641 tagtttttga aggatattga cctttgaagt tgaattttat acttttgagg ggattaatat
     5701 ggaagcagaa atgtatgtgc aggtagctat gatggcaagc aaagttgtaa aaataactgg
     5761 tgtggacgaa gtgataaaaa aattagttaa aaaattttta aataataaag tagaagaagc
     5821 gcttggaaaa caaaggagta aaaaagaatt aaaattaata gaaaataact ttaatgaata
     5881 tatggaaaga agttataaaa attatatata catgaatact atagtattta gaaatcaata
     5941 aaaacaattg atgatttata tataccatta acagtttcaa agcgtaatat ttttgatgct
     6001 tcttgtgaag aagatgtaac tgatatttta atcaatagtt ataaaagatg actttttacc
     6061 aaagtacaaa aagtttttgt tgatagacca agcaggtatg ggaaaatccg actatagcta
     6121 aatgattatc taagtcaaaa cttctattta tctctagttg tttgttattt acaagtcttt
     6181 tcagtatata agtcttaaaa cacatttata tcacccctta agcactaaga cattcttgca
     6241 agcattcctc tgtttcctct aatgtagaaa attcatcaac atattggtcg aagtttctaa
     6301 tatattcagt cttatattta gtgctattca tttctataaa agcaatagtt ttaccattat
     6361 cataatatct gcttattaag acatattttt gtggggtaga tacaatttcc ttatcttcta
     6421 ggactatgtt ttcaacattg aaattaggtg ccatgctata gttacctact cttatgtatt
     6481 cataatcatc actgaaacgg attttatcac gtctaacaga gtcatcttct ttataagtta
     6541 taagttttat cagttctttt aataacttct atcgtgtata tgcagtcaag gtcacatata
     6601 cttcttgttg cataagtttt acctacttca aattttatca ttttaaatac atccaataat
     6661 taatttgtta agtttattgt acactataaa gtgttgttta taaatataaa aatgaaatta
     6721 aataattttt tttgtaaatt aaatacagat aaaaaaaact atatagtgta taattatata
     6781 gaaaagcgag gtggctatgg tgtcaatttc atttggagaa aagataaaaa taatattaaa
     6841 aagaaaaaat atgactatag gtgagctagc agaaaaaaca gggcaaacaa gacaaaatct
     6901 ttcaaataaa ttttcaagag ataatttttc agaaaaagaa attagaaaat ttgcaaaaat
     6961 acttgagtgt gaatttgatt tttattttat tattaaagat acaaatgaaa aagtataaaa
     7021 taggaggaaa tttttatatg gaagtaactt tgcatacagg agagattaat ttttttaaag
     7081 aacatgaggt tgcaaagaat tatataggag aattatacaa ttttatagag gaatataagt
     7141 tatcaatgga aacagtatct attttgacta atatagatat aaagaagtta aatgactttt
     7201 atataggaaa gggaactcta cactatgatg aattagtact tataaacagc gttattataa
     7261 tgccttttat agatgtattt aaaactgcta aacataacta tgaaatgata attaaaagca
     7321 gtaaaaaata tgaagaagat taatagaaaa aaggtagatt ctatataagt agtagctacc
     7381 ttttattgtt gttgataaag ctggtgataa gttatggaaa actcatataa aaattgtgga
     7441 taaaatattt tactgacaaa agttaaaaaa ataaatgctg taaatattat aaaatgttga
     7501 aaaatagcca tatatatata atataagtaa cactataggt aaattactgt attt
//
LOCUS       DAMJVM010000134         7483 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.139, whole genome shotgun sequence.
ACCESSION   DAMJVM010000134 DAMJVM010000000
VERSION     DAMJVM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.139"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            80..901
                     /locus_tag="PC523_003252"
     CDS             80..901
                     /locus_tag="PC523_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDF2476741.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKNLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGIPIEKIEKEVPFAGILLKNNEDIESMQRHL"
     gene            922..2118
                     /locus_tag="PC523_003253"
     CDS             922..2118
                     /locus_tag="PC523_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDF2476742.1"
                     /translation="MFIEFFYLLRSRGVNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDTVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWVLKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFRRKYSKTVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            2284..5682
                     /locus_tag="PC523_003254"
     CDS             2284..5682
                     /locus_tag="PC523_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF2476743.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNTLLTYPEIEFIEKPFVLQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSNGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELKMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLRNNITEEVEF
                     IVGEGERILNVNIWPDFVDDFSVHLVNPSNNQTQTISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWRIIFEPINIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKVARRLSNQSY
                     PNNSSGFGFLNLSDVDLYTLSSINQDLETEDIGYRSINKSFMDEANNYIPIDGYNVQI
                     HNGLEKEKYISKNISRQSGILSGIDVVHTPEFEEELAGLGQSQNFLKISDSLGVLSID
                     NTDYNSIQQVLQLPSIIRTIPTTKMTLLGEINRGTFGGVVATEEIGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHRDFIYPDGTSKIVYLWDQTKEGNPPDGFYIGTEYTREDIN
                     RAIAANDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGVAEDSELIVIKLGKIEGFYNS
                     AMLFAASQYAYNKAFELGRPLVINVSLGTSSLAGLTNRVNSEKAFFTRGLCVTAGAGN
                     EGNTQTHTSGIISHVGGSVEVELELNEEEEELSLELWLSRPDKADVIIVSPTGEESKT
                     VGVSNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRKLLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINSSLWQSSSRG
                     PTIENRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            5692..7365
                     /locus_tag="PC523_003255"
     CDS             5692..7365
                     /locus_tag="PC523_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDF2476744.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPIDFDET
                     ILNNIIQVAWWEESAPMSSLIEITNNVDNGETITTAAGTEYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILAGNGRVNSKYRGITTESDLIVVKLKSYIGTYYAGRINYSV
                     SDFLAAITYVTNIARTENRPLIINLTIGVKSGAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYAPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWTLRLTSELI
                     IGGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVAPGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQNNI
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
BASE COUNT         2863 a          860 c         1428 g         2332 t
ORIGIN      
        1 ttacattatt gttttatatc aaaaatagta ataaagtata tatacattta gaaaatactg
       61 tttaaaagga gtagaattta tgataaaaaa agagatatct aattttagag gaagtagtga
      121 ttatgttgta tcaccagaat tgatggcatc agttaatgtt gctattgcac ttgaaaaacc
      181 acttttgatt aagggagaac ccggaacagg taaaactatg ctagcacaag caatttcaaa
      241 tgaattaaaa aaaaatttag taatttggaa tataaaatca actacaaaag cacaagaggg
      301 gctttatgta tatgatactg ttcaaagact ttatgatagc caatttggag gagaaggtgt
      361 agatgatatt agcaagtaca taaaatatgg taaattagga gaggctttta gttcaaatca
      421 acaagtaatt cttttaatag atgaaataga taaggcggat ttagaatttc caaatgactt
      481 gctttgggaa ttggataaaa tggaatttta cataaatgag acaaaagaaa ctgtaagagc
      541 gaaacaaaga ccaatagtta ttataacttc aaatgcggaa aaagaacttc cagatgcttt
      601 tttaagaaga tgtatatttc attatataga atttcctgat agagatatga tggaggaaat
      661 agtcaaagtg cattttgata aagtagaaga acacctattg gaacaagtaa tgactacttt
      721 ttattggatt agaagtttaa aggatataca aaaaaagccg agtacatcag aattaattga
      781 ctggatacaa gcattaacat tgagtggaat acctattgaa aaaatagaaa aagaagttcc
      841 ttttgcagga atactactta aaaataatga ggatattgaa tctatgcaaa gacacttata
      901 gttaatagga gctggataat tatgtttatt gaattttttt atttattgag gtcaagagga
      961 gtaaatgtat ctttaaatga atggatgact ttaattgaag cattggataa aggactgtgc
     1021 tattcaaact tttcaaattt ttactatctt tgtaggatga ttctaataaa aagtgaaagt
     1081 gattttgata agtttgacac agtattttta gagtacttta aaggtataga acatcaagag
     1141 gaaattccag aagagattat gaattggctt aaaaaaccag atatagattt agaagagttt
     1201 gaaaggttag aacaaaatcc aaatataaat cttgacaaat taagagccaa attagaggaa
     1261 cgaataaaag agcaagattc tcctcataat ggtggtaatt attggatagg aacaggggga
     1321 acttctgagt taggacattc aggaaaaggg caaactggta tacgtatagg tggaaattct
     1381 acttatggta gagctgttct tgaagttgca ggagaaagaa aatacagaga ttttagagat
     1441 gatgaagttt taaatatgcg tcaatttcaa gttgcactaa agagactgcg tcaattttct
     1501 actagaatag atgctccaaa gacagagctt gatttggata agactattga agagacttgt
     1561 aataatgcag gttatttaaa actgtttttt gagaaaccaa gaaaaaatac agtaaaactt
     1621 ttattactta tggattcagg tggttcaatg agagggtata gcactttatg caatacatta
     1681 tttcagtctg taagtaaatc aaatcatttt aaagatgtaa agatatacta ttttcataac
     1741 tgtatttatg atagattatt tacaacacct gaatgttggt taagtaaatc tattaatact
     1801 gaatgggtat taaaaaatat agataaaaac tataaagtta taattgttgg agatgcttct
     1861 atgtcacctt ctgagttact tcatattggt ggtaactatc gtggtccata taattacaca
     1921 cctggtatag agtggcttaa aaggtttagg agaaagtaca gtaaaactgt atggatgaat
     1981 ccagaactga gagatggatg ggattcaatt aattattggt atcaaactca aagaatgata
     2041 caaagtgaat ttaatatgtt tccactgact gtaaaaggtc ttgagaaagc actgaaaaat
     2101 ttaatggttt caagataaag taaatataaa tgatggtatg atttattcaa aattgtacca
     2161 tcattattta tttttttagt atctctatta taaattatat aattgaaact taaaaattat
     2221 atagtctgat acattttaga cttacataga acatatacat taataataat tttagaagtc
     2281 gaggtgatta taataaatta tgaattaatt gtaaaataca atggagatat attgagatta
     2341 gaagaagaac taggtgtatc agtagagata cttaattcat catatgcaat aataacatca
     2401 agtaatgaag aagatgtaaa tacactttta acttatccag aaattgaatt tatagaaaag
     2461 ccttttgtat tacaaactca ggatgtacaa agtttttcaa gtacaggaat aacagggttt
     2521 aaaaatagaa ctggtttaac tgggaaagga actataattg gtataattga ttctggaata
     2581 gattatactt tacctgtgtt tagagatagt aatggtaggt ctaagatatt atattattgg
     2641 gaccagtcta ttcagggaaa tccaccagaa ggttttagag aaggaacatt atacactaat
     2701 gaagatataa ataatgcaat agatggaagt atgtatatac ctatttcaac aactagttta
     2761 catgggacac atgttgcagg tatttgtgca actattgcaa gtgatgccag aataatagtg
     2821 gtgagagttg gaaatataca aaccgatatt ttttcaagaa gtacagagtt tatgagggca
     2881 attaaattta ttttagatag agcattggag ttaaaaatgc ctgttacatt aaatataagt
     2941 tatggaagca atgaaggttc acatagagga acgtctttat ttgaacaata tatagatgat
     3001 atgtgtttat tttggaaaaa taatatagtt gtagcggctg gaaataatgc agataaaggt
     3061 ggtcataaaa ggattagact tagaaataat attacagaag aagttgagtt tatagttgga
     3121 gaaggagagc gtatactaaa tgtaaatata tggccagatt ttgttgatga ttttagtgtg
     3181 catttagtaa acccatcaaa taatcaaaca caaacaatat ctctaacttc aggtgaaata
     3241 aggaatacct tgggtgaaac tagaataact ggttattttt atcctatagc accatattct
     3301 cttacaagaa gagttacttt gcaattaagt tcaaatacac aaataactcc aggattatgg
     3361 agaatcatat ttgaaccaat aaatatagta acaggtaatg taaatatata tttaccaact
     3421 tctgaaggac taaatagaaa tactagattt ttgattccta ctcaagagct tactgtaaca
     3481 gttcctggaa cagcaagtag agttataaca gtaggcagtt tcaattcaag aacagacata
     3541 gtatctatat tttctggaga aggagataca caattaggcg tctttaaacc tgacctatta
     3601 gctccaggtg aagatatagt atctttttta ccaggaggta caagtggggc attaactgga
     3661 actagtatgg caactcctca tgttacaggg gtttgctcat tgtttatgga atggggaatt
     3721 gtaaatggaa atgatttatt tttgtattca cagaaattga gggcattact tttaaaagtt
     3781 gcaagaagat taagcaatca atcttaccca aataattcat caggatttgg ttttttaaat
     3841 ttatcagatg tagatttgta cactctatct agcataaatc aagatttaga aacagaggat
     3901 attggatata gaagtattaa taagtcattt atggatgagg caaataatta tatacccata
     3961 gatggttata atgtacaaat acataatggg ttagagaagg aaaagtatat aagtaaaaat
     4021 ataagtagac agtcaggaat actatcagga atagatgtag tccatactcc tgaatttgaa
     4081 gaagaattag ctgggttagg tcagagtcaa aattttttaa aaataagtga ttcattaggt
     4141 gtactatcta tagataatac agactataat agcatacaac aagttttaca acttccttca
     4201 atcataagaa ctataccaac tacaaagatg acattgcttg gagaaattaa tagaggtact
     4261 tttggaggag tagttgcaac agaagaaata ggcgtaaatt tctttaaaaa taatcctaat
     4321 attaatataa caggaagagg tactctaata tcaattgcag atacaggaat agactatcta
     4381 caccgagatt ttatttatcc agatggtacc tcaaaaatag tatatctatg ggaccaaaca
     4441 aaagaaggaa atccaccaga tggtttttat attggtacag aatatacacg agaggatata
     4501 aatcgagcta ttgcagcaaa tgaccctagt ttatctcagg acgaagttgg tcaaggaact
     4561 atgttgagtg gtatatgtgc agggcttgga aatgtgaata gtgagtatgc aggggtagca
     4621 gaagattctg aattaatagt aataaaactt ggtaagatag aaggttttta taatagtgca
     4681 atgttatttg ctgcatctca atatgcatat aataaagctt ttgaactggg aagaccgtta
     4741 gttataaatg tatctttagg taccagtagt ttagctggtt taaccaatag ggttaatagt
     4801 gaaaaagcat tttttactag aggtttatgt gtaacagcag gagcagggaa tgagggcaat
     4861 acacaaacac atacatctgg aataatatca catgttggag gctctgttga agttgagtta
     4921 gagttaaatg aagaagagga agaattatca ttagaacttt ggttaagtag gccagataaa
     4981 gctgacgtaa taatagtatc tcctactggt gaagaaagta aaactgttgg agtttcaaat
     5041 tataataaag taacaggttt atttgactta gaagggactg aatattctat aacgtatata
     5101 tatccaacta ctttttctgg acagcaattt actaatgtta ctttaaagaa tgcaaaaaga
     5161 ggtgtatgga agattcgatt agtaggggtt tatattataa cagggagata taacttatac
     5221 cttccaaata gaaaactttt aaaaagtgga actagattta gagaggtaga ccctttttat
     5281 actataaatt atcctgctat tcaagatgat ttaataacta taggagctta caatactatt
     5341 aacagtagtt tatggcaaag ttcatcaaga ggaccaacta ttgaaaacag attaaaacca
     5401 gatatagtag cacctggtgt taatataata gctgcttatc ctgggaatac ctatgcaact
     5461 ataactggta cagcagcagc aagtgcacat gcagctggtg ctgcagcaat gtattttcaa
     5521 tatacttttg ttgatggtag atatccaaat caagcctatg ttcaaaaaat aaaaacgttt
     5581 atgcaagctg gagctagaaa ggattcaaat acagtatatc caaatacaaa ttctggatat
     5641 gggcttttgg atgttagagg aatgtttgat gttttaagat aggagggatt tatggaaaaa
     5701 tcttattgta taatttatca aggtgatata gagtctgcac tacaagaaaa tggaataaat
     5761 aggtatatgg ttttaaatag tcaacttgca gtaatatatg tacctataga ttttgatgaa
     5821 actattctaa ataatataat acaggttgct tggtgggaag aatctgcacc aatgagcagt
     5881 ttaattgaaa taaccaataa tgtagataat ggagaaacca ttacaacggc tgctggaact
     5941 gagtatatct atgaaaatcc atataatgat ataaccggaa gaggaatttt attagcagtt
     6001 atagattctg gtatagatta tttgcatcct gattttataa atgatgacgg aaccagtaaa
     6061 gttttatatt tatgggacca agaagcaaat acaaatccac ctccagaagg ttttattttt
     6121 ggaagtgaat ttacaagaag tgagttaaat atagccataa atagaaatga tggaagtttg
     6181 agtcaagata atataggtac tggaacttta gtttctggta tattagctgg aaatggaagg
     6241 gtaaattcga agtatagagg tattactaca gaatcagact taatagtagt taaattaaaa
     6301 tcttatatag gtacttatta tgctggaagg ataaattaca gtgtttcaga ttttttagca
     6361 gctataacgt atgttactaa tatagcaaga acagaaaata gaccactaat aataaatctg
     6421 actattggag ttaagtcagg agctgtagca actacttcaa tactagatac ttttaatata
     6481 ttgagtagtg ctggtgtagt agtagtgagt ggcgcaggaa atcaaggtaa tacagatatt
     6541 cactattctg gaagatttag tagtgtaggt gaagttcaag atgtgataat acaggatgga
     6601 gatgactatg cactggatat aacactaaat actaatggtc cagataaagt aggtgcacag
     6661 ataatatctc cttcaggcga agttagtcat gacataaggt atgctccaga cttttatata
     6721 tatagaggaa aatttaactt agaaaatact acttatgcaa tgagatttat ttatccatat
     6781 attacttctg gaaaagaaaa tttggaaata agattaagag atataaaacc tggagtatgg
     6841 actttaagat tgacttcaga acttataata ggtggagaat atgatatata cttgccaaat
     6901 aaaaatttga tagctccaga tacaaggttt ttagaccctg attcagtagc tacaataact
     6961 atgtatgctg ctagtgatga tgtaattaca gttggtacgt tcaataataa gactgacagc
     7021 atgtggatag gttcttcaaa aggtccaatc cgaggaagag gaattaagcc agatatagta
     7081 gctccaggtg tagatataat atctacatat aaaaatggaa cgtataatac aggaacagga
     7141 accggtgtga gcagttctat agtcacaggt gttttagctt tactaatgga atatttggaa
     7201 aaacagaata atataccaag attatcattg tttacacaag ttttaaagac atatttgata
     7261 ttgggagcta caaagcttga gatatataca tatccaaatg tatcacaagg atatgggatt
     7321 ttaaacttaa aaaatacaat tcaacaaata gcaaatactc tatagataat aatctgcata
     7381 aatttatagt taattaatag ttcagataac aaaaaattga attagagtaa atataaaaat
     7441 taaaaaatag ttttatatgt tgtatatata aaataactat ttt
//
LOCUS       DAMJVM010000135         7206 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.140, whole genome shotgun sequence.
ACCESSION   DAMJVM010000135 DAMJVM010000000
VERSION     DAMJVM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7206
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.140"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            220..3399
                     /locus_tag="PC523_003256"
     CDS             220..3399
                     /locus_tag="PC523_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2476745.1"
                     /translation="MKIDMIYKLIRRNTEPSRWARGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSNGKNIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSSTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINMDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYDPNIHYFADED
                     EKLVDIIEEYGTNLSYSFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPIRFDLKNIEDKIVLCSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQVLSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DSNICCDVKYIYDDEDKKFVLPNIQKETTIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSDKFKLKKIYNASSIKASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMGTSKNNKIHKNKALYIN
                     DMIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGSKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCILDEAQNIKNPLALV
                     TASVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKIKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSIDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIIDLFN"
     gene            complement(3684..4448)
                     /locus_tag="PC523_003257"
     CDS             complement(3684..4448)
                     /locus_tag="PC523_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2476746.1"
                     /translation="MAYFNYNNKQCYYNEIGDGTPLLFLHGNTASSKMFNEIINFYKD
                     EYKVILIDFVGHGKSQMVDKFAADLWFDEAMQVICFLEVMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWER
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDDFVSLISNDFFENTFSSLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(4687..6180)
                     /locus_tag="PC523_003258"
     CDS             complement(4687..6180)
                     /locus_tag="PC523_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2476747.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLEYNIPSRVQIEVIPKLLNGQNLV
                     VKSKTGSGKTASFAIPLCENINVEYNNIQALIVVPTRELALQVKDEISSIGRLRKVRC
                     SAIFGKQPIKEQIAELKQRVHIVVATPGRIIDHINKGTIKLEDIKYLIIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVGLFSATIDEEIKYICEKYIPDYSVINIEENKSDTNQRTR
                     QIDDKIIKTNEREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKKQKFNVLVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKRNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEKSRITHGKILFEEYSKE
                     ILKEVSKRKKADKNSYKGKDIKLNTESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKHKEIPIKKKMVKVKRDIR"
     gene            complement(6509..7051)
                     /locus_tag="PC523_003259"
     CDS             complement(6509..7051)
                     /locus_tag="PC523_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2476748.1"
                     /translation="MKICITVGHSILKGGKSTGASGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDIIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGN
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
BASE COUNT         2672 a         1022 c          783 g         2729 t
ORIGIN      
        1 ataataaaat tttatattta ctaataatgt atttatataa ttatttttca ctcatagttt
       61 ataagataat tcaacaaaaa tctaatagta aattgttatt tatacatatc ttgcaataga
      121 taaaagctat caatatctgc cctaatttta gtcttaaatt tagagtgtaa attgaatgca
      181 tttagattat aatatataca gtgaggtaag gtgaataata tgaaaataga tatgatatat
      241 aaattaataa gaagaaatac agaaccatca agatgggcaa gaggtgtaaa atattacacc
      301 aatcaactag ttgattatat agacatagat attgatgacc tacaaaataa tctgctgaca
      361 tttcgtggtg gagtcaattc tgaagataat tatagctttt attttaactc aataactata
      421 gatataaagc actttaagat aattgaaaat gattgcagtt gtgctgactg tcaatcaaat
      481 ggtaaaaaca tttttatatg taaacatata gttgctattg tgataaaaag cttaaaacaa
      541 ataaaaaata aaaaagatgt tcctcctatt ctaatagata tattaggtgc agataaggat
      601 aagctctcta gtacaaattc taaaaccagc ttagatttaa gcaagcaatt actttctttg
      661 ttagataata ctaaaagaga aagggttaat ttagatgtat tgataaatat ggattcaaat
      721 aaagcaattg ctgaatttaa aataggaatt gataaaatgt acattttaaa aaacattact
      781 gaatttgcat attcaaaact taatggatat gaattagatt ttgggaaaaa atttacttat
      841 gatcccaaca ttcattattt tgctgatgaa gatgaaaagt tagtagatat tatagaagaa
      901 tatgggacaa atctttcata cagtttttct tcatcacact caaaaaaatt tatggagata
      961 aatgcaagct ctttaaaaca atttttaaat gctattaagt acaaagattt tacccttatt
     1021 ttttctaaag aagaatatca cccaaaaata ttaaatgata ctctacctat aaggtttgac
     1081 ttaaaaaata tagaagataa aatagtctta tgttctaaag attcactacc tgttccactt
     1141 actaaaaaaa atgatgtact tttatatgat ggcaatatct atttgttatc ccataaaaaa
     1201 gctcatgatt attcaaaaat atatcaagta ttatcaaaga ctaaagagct aaaatttgat
     1261 aaagaggatt ctaaggatgt tttaggatta ctagttccta gattaaaatc tatatctcaa
     1321 gaagttcatt tagatgataa cataaaaaat aatataacaa aagattttaa atctgagttc
     1381 tattttgata tgattgatag caatatatgt tgtgatgtaa agtatatata tgatgatgaa
     1441 gataaaaaat ttgtacttcc caacatacaa aaagaaacta ccattgaaaa taaactgact
     1501 tcttcacaat ttacaaaaga aaataatcat tatgtgttta aaggaactga ccacgaccta
     1561 tttacatttt tagatttaca attagaatct ctaaaggaat ttggagatat atattattct
     1621 gataagttta aattgaaaaa aatatataac gcttcatcaa ttaaagcttc aatcaatcag
     1681 actaaccaga attatctaga atttaatttt aatatatctg atataaatcc aaaagagtac
     1741 aaagacattt taaaagcttt tcaagaaaaa cgcacttttt ataaacttaa agatggaaac
     1801 tttatagatt tatcagaaag agaaacaaaa gatttttttg aattagttga aaatctagat
     1861 ataatgggta catcaaaaaa taataaaatt cataaaaata aagctctata tataaatgat
     1921 atgatacgta gtaaaaattt gaagtttata gatggtaaaa aagaactaga cagtatatgt
     1981 gaaaaattta gaaactttga tagtataaat ttagaaattc ccgcaaattt aaatgcaaat
     2041 cttcgtgact atcaaataaa tggtctcaac tggtttaaag ttttagatta ttataaattt
     2101 ggtggtatat tagctgatga aatgggtctt ggtaaaacaa tacagactat tgcattttta
     2161 ttatcattat ccaataaaaa aagtcttatt gttacaccaa catcactcat ttataattgg
     2221 aagaatgaat ttgaaaaatt tgctccagat ataaaagttt tattgataca tggaagtaaa
     2281 agagatagag aaaaatgttt tatggaatta gaaaattttg atgtaatact tacaacatat
     2341 ggtactctta gaaatgattt agaaaaatat agtgaaataa aatttgatta ttgcatatta
     2401 gatgaagctc aaaatataaa aaatccatta gctttagtaa ctgcttctgt aaaaagtata
     2461 aatgcagaaa ataaatttgc actcactggt acacctatgg aaaataatct cctagaatta
     2521 tggtctatat ttgattttat aatgcctggc tatctatata gtaaagcaaa gtttcaggaa
     2581 ttatttataa ataaagaaga taatgttaaa aatctgaaaa aattgataaa accttttatt
     2641 cttagaagaa gtaaaaaaca agttatgaaa gaacttccag ataagattga gaaaaacttt
     2701 tttgttgaat tgaataaaga gcagaaaaag atatatagtg tatattcaaa ggatatacaa
     2761 gataaaataa aagataaaaa tctaaagaaa gataaaatag ttatattttc ttatttaact
     2821 aaactaagac aactttgttt agacccaagt atagtagtaa aagattacaa taagaaaagt
     2881 tcaaaaattg aaacatgttt agaaatactt agagatagta taaacgaaaa ccacaaaata
     2941 ttgttgttct cacaatttac ctctgtgctt aagaacataa gtaaggaact tgataagtat
     3001 aaaattaaat accattatat agatggtaaa actaatgcta aagaaagact tgaacttgta
     3061 gatgagttca ataacagtat agataaaaaa gtttttttaa tatctttaaa agcaggtgga
     3121 acgggtttaa atcttacatc tgctgacatg gtaattcact ttgacccatg gtggaatcca
     3181 tcagtagaaa atcaagctag tgatagagct catagatttg gtcaaaaaaa ttcagtacaa
     3241 gttataaaat tgattgctaa aggaactata gaagaaaaga taataaaatt acaagaaagt
     3301 aaaaaagaac ttataaatca atttataaat ggagaacttt ctaatgaagg tgtattaaaa
     3361 agtctatctg atgaggagat aatagactta tttaactaaa ttatttaatc ctacaactta
     3421 actatataaa tttattctaa gatttatata gttaagctac aatacttgaa taaataaaac
     3481 ttcatattat tatttaatcc caactttata atccaataat atctctttct acacctattg
     3541 aatatacaaa tgtaataact taactattag aatattttgt tttaacaatt ctatttcatc
     3601 ataagaataa agtatatgta cacttacttt tgtaagtttt acaatctatt ttatagtttt
     3661 cattattata aatttgggtt aaatcattct tctaaaaatt tctttgctac atttgaaaaa
     3721 tctaatccat tcgataaaat tgcaggatga ccacctttat caaacaaata cattttacca
     3781 tttttaatct tttcaagaag tgatgaaaaa gtattttcaa aaaaatcatt agatattaaa
     3841 gatacaaaat catcttctct acttcctgtt aaaagcacct ctggttgcat agtttctaga
     3901 ggtttatgaa aaaattttcc tatagtttta taatgttcaa aaatagcttc tgtatcatta
     3961 tctacaactc tttcccaatt ttctccttga ttataaatat aaaaagcttt tgcaccatca
     4021 tcttgctttg atgcttcacg ctctattttc acattttgta caaatgactc tagaggaact
     4081 tctccttcaa aactatccgc aattacctta ttcactaaat caggtctttc aagtgcaaca
     4141 tttaatgcaa caagagctcc tccactacta ccaattatgt ttactttttt atagttcatc
     4201 acttctaaaa aacatatcac ttgcatagcc tcatcaaacc ataagtcagc ggcaaactta
     4261 tcgaccattt gagattttcc atgacctaca aaatcaatta atattacttt atattcatct
     4321 ttataaaaat tgattatctc attaaacatc ttagaagagg ctgtatttcc atgtaaaaaa
     4381 agtaatggtg taccatctcc aatttcatta tagtagcatt gtttgttatt atagttaaaa
     4441 tatgccatat atttccctcc ttttatgttt ctataaacat atatttatta cttatattta
     4501 aaatgactat tagcataatc acttaaaaat agtatagcct agaatatagg taaaatcaac
     4561 attcttttac ttaacaactt ctattgtttt tttgcaactt catttagata acagagcaat
     4621 tcatcaacat tgatactttt acttaaacct acgatttctt catcagaaag agcagtaata
     4681 actaaattat cgtatatctc ttttcacctt aaccattttt ttctttatag gtatctcctt
     4741 atgttttttt aatattaaat ctcccttata atttaatata tctacatatg aacataaatc
     4801 ttgaacttca attacaccta tatcatcatt agtaatacct tttatattac taaatgcacc
     4861 aacgatatct aagactctta ttttcttctt cttaccagca tttaaatata atttaacaac
     4921 ttcactttct gtatttagtt ttatatcttt acctttgtaa gaatttttat cggctttctt
     4981 tctctttgac acttctttta ggatttcttt tgaatattct tcaaataaaa ttttgccatg
     5041 cgtaattctg cttttttcta tatcttttaa ttcatttatt ttgtatccta tatacgtttc
     5101 tatattctct atatattttt catccttttc agttactata gtaattgcct ttccactatt
     5161 tctcttccta ccagttctac ctattctatg tatatagttt tctttatctt gtggcacatc
     5221 ataatttatt actaaagaaa tatcatcaat atgtattcct ctagacgcta catcactact
     5281 aactaataca ttaaactttt gttttttaaa atccttaatc acaaaaattc ttctttcttg
     5341 cgataaatct gcatgtaact ctcttattaa aaaaccttct ttactcatct tattatataa
     5401 tttagatact ttctctttag tattacaaaa tattatcaca ctctttggat tttcgctgta
     5461 tattaattct tttaaaagga tatacttctc tctttcattc gtcttgatta ttttgtcatc
     5521 tatttgtctt gttctttgat tagtatcact cttattttcc tctatattta taacactgta
     5581 atctggtata tatttttcac atatatattt tatctcttca tctattgtag ctgaaaaaag
     5641 acctactatt ttctctttag gtaaatttaa taatattttt tccatttgtt caacaaaacc
     5701 tttattaaac attttatcgg cctcatctat tattaaatac tttatatcct ccaacttaat
     5761 agtaccttta tttatatggt caattattct acctggagta gctacaacaa tatgaaccct
     5821 ttgctttaat tcagctattt gttcttttat aggttgtttg ccaaatatag cactacatct
     5881 cactttcctt agtcttccta tactcgatat ctcatctttt acttgcaaag ccaattctct
     5941 tgttggaact actataagtg cttgaatatt attgtactct acatttatat tctcgcataa
     6001 aggtattgca aaacttgctg tctttccact tcctgttttt gacttcacta ctaagttttg
     6061 accatttaat aattttggaa taacttctat ttgtactcta gaaggtatat tatattctaa
     6121 actcttcaat gacttaagta tcttttcatt taacttaaat ttttcaaatg tatatttcat
     6181 atattaccac cttattatat ttatcttatc tatgtatttt tctacattat attattataa
     6241 tacaaggttc tactattttt atattattta ttagagtgaa gataaaaata tatactatgt
     6301 aaagctataa tttagtagta aataaattaa atttttatat ttaaatacct ccttgaaaat
     6361 actgataaat caatattttc aaggaggtat cccttgtcaa caactaaact actaatatat
     6421 cttttacaag tttaaattat actaatatat tgtattttat ttatatctaa tattaaaggt
     6481 attaatcaat acctaaatca ccataatctc acatatttat atctttattc aatatacctt
     6541 ctgtaatcaa tcgtgctata taatcataac ctgattcatt tgctttgtta caatcattca
     6601 cattatcaca gaaaaaactt tcaatctgta ttgcaactgg atttgtattt ctcaatatat
     6661 ataaattttc tcttttttta gctcctctat tttcaaataa ttctccaagt ctattagata
     6721 ctcttttagc atattctaat ccttcattac catagtaaag cacttctgta ccacattgtg
     6781 ttccatcagc tttatttaaa tgtagctcta ctaataaatc gtatttacca ctgtttactt
     6841 ttggaactct atagaaaaat tcttctcttt ctgacataaa gtgtctttct ggacaaatta
     6901 ttatatctac tgtattacct tgacttataa gcatctcagc taacatagga gctaattttt
     6961 tgttataatt gtattcatca actataccac ttgcacctgt acttttacca ccttttagta
     7021 tactatgacc tactgttatg catattttca tttacttttt tcaccttctt ttaagccaat
     7081 ttttatataa tagtaatata atcttaaagt tattactaat aataatatat taattggaga
     7141 aggtaaaagt tctccgtgat tatcattttt ttaacatatt tgtcttattt tatcataaat
     7201 acatat
//
LOCUS       DAMJVM010000136         7143 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.141, whole genome shotgun sequence.
ACCESSION   DAMJVM010000136 DAMJVM010000000
VERSION     DAMJVM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7143
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.141"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            109..756
                     /locus_tag="PC523_003260"
     CDS             109..756
                     /locus_tag="PC523_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HDF2476749.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEIGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESVCAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(898..1467)
                     /locus_tag="PC523_003261"
     CDS             complement(898..1467)
                     /locus_tag="PC523_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476750.1"
                     /translation="MKKFTICIIMFAISALVLAGCTNDNKIKNEVDNMFNAIKTQDSD
                     AVDKYFPKLGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKVNNTDMVSIL
                     KKNPPKLGAMMPNPTMKDIDSANKKEFNLTLELVKSGDNYVCEVNQESAMKLLNVITG
                     GGTDYLADYNKDYYKQLDEEQKNYENQNN"
     gene            complement(1472..2371)
                     /locus_tag="PC523_003262"
     CDS             complement(1472..2371)
                     /locus_tag="PC523_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2476751.1"
                     /translation="MKLLSNINNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKRLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLYKIPFPVKIKKVYGDKYLKYLKYLVLIIFVIILPVAIVNVAGGGDPWFCKW
                     ICPSGTFLGGIPLILGNENLRESIGFLFSWKLSILLLILLISIMIYRPFCRYICPLGA
                     IYSFFNPISIYRFKIDKDKCTNCTACQAKCKLDIPVYKDVNSLDCIRCGECIKACPHN
                     AITTTFSKDKKENKFCKKKYRGM"
     gene            complement(2382..2531)
                     /locus_tag="PC523_003263"
     CDS             complement(2382..2531)
                     /locus_tag="PC523_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476752.1"
                     /translation="MSNFIKDNKTGISIIVISFIFIVLGTYRGESNTVLTKAIMICLE
                     CIGIG"
     gene            complement(2524..3111)
                     /locus_tag="PC523_003264"
     CDS             complement(2524..3111)
                     /locus_tag="PC523_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDF2476753.1"
                     /translation="MKRFFTLVLGCIFVLGVVSGCNKKTETNLVETNKNNSEWFNNLV
                     TEDVDGNKVTKDVFSSKELTLINTWTTWCGPCIGEMPELEALSKEYENNNSNVAIKGL
                     IVEVDGTDMKTGLSDKEKELVKDIMKKSGATYQQLTVSEGLKKTDFKRTIEFPTTYFV
                     DKKGNFVGEKVTGANSKEEWKKIIDERLKMVKDSE"
     gene            3534..4859
                     /gene="cdeA"
                     /locus_tag="PC523_003265"
     CDS             3534..4859
                     /gene="cdeA"
                     /locus_tag="PC523_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HDF2476754.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQNEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATNKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGILYFLSSKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGVTTFLFNIVALKLAGESGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIVALVFA
                     PFIVRVFVNPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNLTGLWLTVPFAEVLTIFISILFIKKYKGRYKY"
     gene            complement(4954..5748)
                     /locus_tag="PC523_003266"
     CDS             complement(4954..5748)
                     /locus_tag="PC523_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2476755.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIMGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYNIELSVLLKRDMIWTSISALIEPSTSSISLTYLNYF
                     VQICLNEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(5749..6624)
                     /locus_tag="PC523_003267"
     CDS             complement(5749..6624)
                     /locus_tag="PC523_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2476756.1"
                     /translation="MSSSIKIENLYHSFKKQNVFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQVFEFSSS
                     FYKNYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKSIKPKKAFGNTVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(6628..6996)
                     /locus_tag="PC523_003268"
     CDS             complement(6628..6996)
                     /locus_tag="PC523_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2476757.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFIKNLLQEA
                     KKLEINREELVNIIINFKED"
BASE COUNT         2445 a         1103 c          814 g         2781 t
ORIGIN      
        1 ttaggaaaat attaattgta aaattaatgt tatacaataa tatatcgtat aattttaatt
       61 aacataatag ttacaacatt aaaatttaaa tatgggagtg attttagtat gataagacaa
      121 acaccattag aaaaacgata tgacaagcta ggtcctcata ttgtaacagc actcaaaaaa
      181 cgttattttg atgcttatta ctgtaaaact agaaatgaag ctttacaaca aattttagat
      241 ttaattcctc aaaatcatgt tgtatcatgg ggaggctctg aaacgcttaa ggaaattggt
      301 gtacaaactg ctgttagaga gaagggattt aaagttattg atagagattt agcaaaaact
      361 ccagaagaaa gaacagaaat tatgcgtcaa gcacttttat gtgatacttt tttgatgagt
      421 agcaatgcaa ttagtgaaga tggacaacta tttaatattg atggaaatgg taatcgtgta
      481 gctgcaatga tatttggtcc aaaaaatgtt atagttgtag ctggtatgaa taaaattgta
      541 aaaaatattg atgatgcaat acaaagaact agaacaattg catctccggc agttgttcag
      601 cgttttcaag atgtcaatac accttgctat attaatggaa gttgtataga ctgcacaagt
      661 cctgaaagcg tttgtgcata tatggttact acaagaatca gtcgtccagc aaaaaaaata
      721 aaagtaatat tggtggggga aaatcttggt ttataaaata tacatacata taaatagctc
      781 tataaataat aaaatcatat gaaataactt attcctaaaa gtggtagaat gaaagccact
      841 ctgcaagact ttcattttac cacttttaca ttcttttaaa tttatgcttc tatattatta
      901 attattttga ttttcataat ttttttgttc ttcatcaagt tgtttgtagt aatctttatt
      961 gtagtcagct aaataatctg tgccacctcc agtaattaca tttaacaatt tcattgcaga
     1021 ttcttgatta acttcacaaa cataattatc accagattta acaagctcta atgtaagatt
     1081 aaattctttt ttatttgcag aatcaatatc tttcatagtt ggatttggca tcatagctcc
     1141 tagtttagga ggattctttt ttaaaattga aaccatatca gtattgttaa cttttaaatc
     1201 tacagtcact ttatccttat tattctttat atctgaaatt tcccatgtca tatttttaga
     1261 atacatttta aatccctctt tatcttcata tataagatta cctaatccta attttgggaa
     1321 atacttatct acagcgtctg aatcttgtgt ttttattgca ttaaacatat tatccacttc
     1381 attttttatc ttattatcat tggtacatcc agctaatacc aatgccgata ttgcaaacat
     1441 tataatacat atagtaaatt tcttcattaa attacatccc cctatacttt tttttacaaa
     1501 atttattttc ttttttatct tttgaaaaag ttgtagtaat tgcgttgtgt ggacaagctt
     1561 ttatacattc gccacatctt atacaatcta aactatttac gtctttatat actggtatat
     1621 caagcttaca ttttgcttga catgctgtgc aattcgtaca tttatcttta tcgattttaa
     1681 atctatatat tgaaatagga ttaaaaaaac tataaattgc tcctaaaggg catatatatc
     1741 tacaaaatgg tctataaatc atgattgata tcaataatat caatagaaga atgcttaact
     1801 tccaactgaa taaaaatcct attgattccc ttaaattctc atttcctaaa ataagtggta
     1861 ttcctcccaa aaaggtccct gatgggcaaa tccacttaca aaaccaagga tctccacctc
     1921 ctgctacatt tacaatagca actggcagaa taattacaaa tattattaag accaggtact
     1981 ttaaatattt taaatattta tcaccataaa ctttttttat ctttactgga aatggtattt
     2041 tatatagtaa atcttgcaca agtccaaatg ggcaaagcca accacaaaca aatctaccaa
     2101 aaaaggctcc aaatgccatt aaaaaaccaa caacataaaa tgagaattta tactctctgc
     2161 ttgataaaac tgcttgtaat gaacctattg gacatgctcc aagcgcgcct ggacatgaat
     2221 aacagttcaa tccaggtaca cacaatcgtt tactttctcc tccatatata gttccttcta
     2281 aaaatccatt tacatatcca tttgtcaatg ctgtaaatgc aacttgagta ataagcctta
     2341 tattattgtt tatattagat aataacttca taccttttct cctaaccaat accaatacac
     2401 tctagacaaa tcataattgc tttagttaat actgtatttg attctccacg atatgtacct
     2461 aatacaataa atataaaact aattacaata atagaaatgc ctgttttgtt atctttaata
     2521 aagttactca ctatctttta ccatttttag cctttcatca ataatttttt tccattcttc
     2581 cttgctattt gcacctgtta ccttttcacc cacaaagttt ccttttttgt ccacaaaata
     2641 tgttgttgga aactctatag ttctcttaaa atcagttttt ttaagacctt cagatacagt
     2701 caattgttgg taagtagcac ctgacttttt cataatatct ttgactaatt ctttttcttt
     2761 atctgaaaga ccagttttca tatcagttcc atcaacttct acaatcaatc cctttatagc
     2821 tacatttgaa ttattatttt catattcttt tgataaagct tcaagttctg gcatttcacc
     2881 tatacatggc ccacaccaag tagtccaagt attaataagg gtaagttctt tacttgagaa
     2941 tacatctttt gttaccttat ttccatcaac atcctctgtc accaaattat taaaccactc
     3001 actattattc ttgtttgttt ctaccagatt ggtttctgtt tttttgttac aaccagaaac
     3061 cacacctaaa acaaagatac accctaaaac taacgtaaaa aaccttttca tacctcacac
     3121 tcctataatt tatatagtat ataaaatcgt cgtttgaaat aaaaataact tatcttctat
     3181 aattaaagtc attttattta ataaataaga gtcaattaaa tcaaattgta taaaagatat
     3241 acagttaaaa tatatttctt ttcagacaaa atcttatatc taaataatag attgtaattc
     3301 ttaagattat atacatatat ttctaaagat tttcttaaga aaaatttaga tttcactaat
     3361 acaatatacg tacttttaat aatataagct tattaattag cattatacta attcatttca
     3421 ctatgatttt ttgttgattt taagctgttt accatatgaa taaagttttt cttgactata
     3481 tagttactat atagcttatt atatatagag agtaacaaaa ggaggtaaaa tttatggaaa
     3541 atttatttac aagaaaattc actacttttg aatttctaaa atttgtttct cctgcaatta
     3601 tatccatgat atttatatct ttgtacacaa taatagatgg catctttgta tcgacattag
     3661 ttggttcaga tgctcttgct agtataaata ttgtactacc tataattaat cttgtttgtg
     3721 gatttggaat aatgatggca actggtggag gtgctatcgt ttctatacgt atgggtgaaa
     3781 atagacagaa tgaagccaat tctacatttt cttttatagt tttgttttca ttgattgttg
     3841 ggattttatt cacagtaatc tcatatttct ttatcaaaga aatatctatt ttgcttggag
     3901 caacaaataa gttactacca tattgtataa cttatggaaa agttatgatt ttatgtacac
     3961 cattttatat tttaaaattt atatttgaat actttgcaag aactgatgga aattctaaat
     4021 ttagtttatt tctatcagtc attggtggtg taacaaatat aatattagat tatgtattta
     4081 ttaaatattt tggaatgggt cttttaggag ctgcagttgc aactgctata ggtattattt
     4141 taacttgtgt tttaggcatt ctttacttct tatcgagtaa atctacacta aaattaagaa
     4201 aaccaaaaac cgattttaga cttataagag atactatgat aaacggttct tctgagatgg
     4261 tcacagaact gtctacagga gttacaacat tcttatttaa catagtagcc ttaaaattag
     4321 caggagaaag tgggcttgct gctcttacca tagtattgta tgctcatttt ttaatgacat
     4381 cagtctatct aggatttgct gctggagtgt ctccattaat aagctataat tttggtgctg
     4441 aaaatagtga taaattaaaa gagacattta aacattctct aaaatttata tttgtttctt
     4501 cccttttagt gtttattgtt gctttagtat ttgcaccatt tattgttagg gtctttgtaa
     4561 atccagataa cacagtattt aagctagcct tgcaaggatt aaaaatattt gcatttgctt
     4621 ttttatttgt tggtataaat atatttgcat caggcttttt tacagcattt cacaatggaa
     4681 aaatttcagc tattatatct tttagtcgtg cctttgtttt tataatcata ggaattataa
     4741 ttcttcctcc tatgttgaac ctgactggat tatggcttac agttccattt gctgaagtcc
     4801 taactatatt tatatctatt ctatttataa aaaaatataa aggtagatat aagtactaaa
     4861 aattaatatt taaaatcaac atttattaaa actaatgaac ctgctttaaa atacaaattt
     4921 ataaatttga tttttaagca ggttttattt tagttataat aagtcatttg catactcttt
     4981 aattggtgct tttctaagta ataaaattat aaatattatg caaataacta taattcctac
     5041 ttttgttcca aataaaataa acggattctg aaatgaataa ataatttttt ctaaatacaa
     5101 tagcatttta tgcatatttt tagttgcaaa attaactata taaattacca ttaatatcgg
     5161 taatcctatc aatatagtaa tactagtaac ttttctaact ctatatgtaa aagctccaag
     5221 taatactcct ataaagccta tacatatttc atttaaacat atttgtacaa aataatttaa
     5281 ataagttagt gatatacttg atgttgaagg ctctatcagt gctgatatag atgtccaaat
     5341 catatctctc tttagtaaaa ctgacagttc tatattgtaa cctgttataa tttctataaa
     5401 taacttcgat aaaaaaatca aaactatacc taaaatagaa atcacaaagc ttaaaataat
     5461 taaatctatg acaatagctt ttatacaact ttttctatct gctcttatac ttaaagctcc
     5521 tgaaaagtct ttatttgcta tatttactcc atacacaaat agtagtatag ttatatatgt
     5581 taaaatagga ttcacaatcg tggataaatt agttttagat gcttctccaa aaatcttgag
     5641 gataaacact ggtaaaatat tcataacaat tcccataatc ataaatataa ttatatacaa
     5701 tttagtattt ttattaaaaa attttatata tggctttaat tcattcaatt acaattcctc
     5761 cttcttagtc atgttaacaa acatatcttg tagccctatg tatcctacat caatatttga
     5821 atttttaagt attttttcat cattttcact aaaatcacca tagtaaaaat aagcaactgt
     5881 atttccaaat gcctttttgg gctttatact ttttatacat tctaattttt ctaactcttc
     5941 tttatttcca gtaatataat gagctttttg ttttatgaca tcaatttctt catctataat
     6001 aatttttccc tcattcaata ttattacatg ctccagtaaa tcatctattt catcaatcaa
     6061 atgagttgat attattattg tccttggatt ttttatataa ctatctaaaa ttatgttata
     6121 aaactcttgt ctatttactg catccaaacc aattgttggc tcatcaaata tagtgatagc
     6181 tgaattagaa caaataccta ttatatttga aagtatggtt ttcatacctc ttgacagttg
     6241 tttatacttt tttttagtat ttaagtcaaa atatttgcat aattcttctt ctaagccttt
     6301 atcataattt ttataaaaac tagaagaaaa ttcaaatacc tgacctactt taaaatctgg
     6361 actataaaat tctttttccc tgacaataca aacttcttcc aaaactttta tatcttcttt
     6421 tgggttcttt cctaatattt ctatctcacc ttcattttga attaactgat ttaccattat
     6481 atttaataat gtggtttttc cagctccatt cttacctaat aaaccatata tcttattttt
     6541 ttcaaaattt agagacatat tattaaaaac attttgtttc ttaaaactgt gatataaatt
     6601 ttctatctta atagaactac tcatatatta atcctccttg aaattaatta taatattaac
     6661 taattcttct ctatttattt ctaatttttt agcttcttga agtagatttt ttataaaatt
     6721 atgtttaaaa ttttcttttc tggcttcttt tataatagtt tttgccccat ctgatacaaa
     6781 catccctatt cccctcttct tatataaaat attcttatca accaatatat ttataccttt
     6841 taatactgta gctggattaa ttttataaag ctttgaaagc tctgttgttg atggaacctg
     6901 ttcttcttct tttattccat ttgatagtat ctcatcttca attgctcgtg ctatctgaat
     6961 gaatataggt tcttcattgt ttaatactaa tttcataggc ttctcctttc taagttagtt
     7021 aattactcaa ctaactaact gtaacactat cttatagtat aatattacat ctgtcaatac
     7081 gaaataaaaa aatatatgca aacttttatc aaaaatacgt ttttatagat actaaaaaat
     7141 att
//
LOCUS       DAMJVM010000137         6732 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.142, whole genome shotgun sequence.
ACCESSION   DAMJVM010000137 DAMJVM010000000
VERSION     DAMJVM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6732
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.142"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            24..558
                     /locus_tag="PC523_003269"
                     /pseudo
     CDS             24..558
                     /locus_tag="PC523_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
     gene            757..1920
                     /locus_tag="PC523_003270"
                     /pseudo
     CDS             757..1920
                     /locus_tag="PC523_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(2058..2657)
                     /locus_tag="PC523_003271"
     CDS             complement(2058..2657)
                     /locus_tag="PC523_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476758.1"
                     /translation="MSQNIDFNKSNFYLRLDERLAVPLKYAYVSTILKFQKWRGCTFD
                     FSNTKLEKLKNIIVHGDNDNYNKLLMVIKKRYDDILDEEFWHIEIKELPYIDRGFKSN
                     KDIITTFIHGKYYPKRKAFRHIDFIKNQYPFKEYCSKQEDNSTNEIKIDFYITKECHY
                     KVWCIENIDIQEHIWCELVNVSLSEEYRKLFGEILDSSK"
     gene            complement(2683..2982)
                     /locus_tag="PC523_003272"
     CDS             complement(2683..2982)
                     /locus_tag="PC523_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476759.1"
                     /translation="MAYSDIYSISCGEKEFPTNITIDEDLLFCVVTCFVLDRFRYDDL
                     NIKSVISYEKHMYDINDYKLTKIENLDFKQNGFIYDKKYYLYNIFIDKSPLNKYK"
     gene            complement(3010..3261)
                     /locus_tag="PC523_003273"
     CDS             complement(3010..3261)
                     /locus_tag="PC523_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HDF2476760.1"
                     /translation="MEKLIQDIKNLDSYYNTSYYTFHLDEPLWKSLKVRNWSGVPYYI
                     KQLCNEVKSLIKERNLTQSEIFQYITDPKKRTHYFFVAQ"
     gene            complement(3476..5407)
                     /locus_tag="PC523_003274"
     CDS             complement(3476..5407)
                     /locus_tag="PC523_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2476761.1"
                     /translation="MFLDRIIMENVGPIDNLDIGFPFNEDGSPKPIIFVGENGKGKST
                     VLSNIVDSLFEFAKQPYNDITETNKDGTTPYFKVLSWKNTKVGCDYSFSFINFVNGEQ
                     NFQYIEKLGNLSFEQCIEKTSGLLNLDNNWGNRKNIKDITKNKEYFEEEFANKSFCFF
                     PPNRYETPHWMNQNIISENLITINDKISGKLNKPIIVQNVTETNIKWLLDIIVDSRAD
                     LIQSDLGGWNSEQDLNNIRLLKIARENVEEILSEIVKKDVKFGLNWRNSGGSRFNIID
                     RSTNSMIIPTLDALSTGESALFNMFSTIIRYSDYGDINKSIKLNEIEGIVIIDEIDLH
                     LHSSIQCDVLPKLIKKFPKVQFIITSHSPLFILGMKDIFKEDGFKIYEMPTGENIGPE
                     EFSEFERSYEYYKRTELFSKDIQDILKSKNGKTLIITEGKTDWKHMKAGLLKLKEQGL
                     YHDLDIEFLEYENDIKMGYNNLINICEAASKLRNNNKVICVFDRDLSDRDLRKVKDEN
                     TNYKLWGNNVISMVLPLPDNRVEIDKIMCIEHYYSDEEIKTFYKGRRLFLGDEFNSKL
                     GLHILGDKICQDKNKCGKKSIIDSGCVVCDIKNPEDNIALSKNDFADSILNGVEEFKN
                     ISFENFKLVFDLIQILVNE"
     gene            complement(5482..5831)
                     /gene="ssrA"
                     /locus_tag="PC523_003275"
     tmRNA           complement(5482..5831)
                     /gene="ssrA"
                     /locus_tag="PC523_003275"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(5989..6444)
                     /gene="smpB"
                     /locus_tag="PC523_003276"
     CDS             complement(5989..6444)
                     /gene="smpB"
                     /locus_tag="PC523_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDF2476762.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
BASE COUNT         2378 a         1033 c          772 g         2549 t
ORIGIN      
        1 ataataatac atggggtaat taattgaaaa aactagtaaa aatattaata aatattattt
       61 gcattattac gctaattttc tctagccttt cgatttacat aaaattatct gaatataaaa
      121 aggctgatga agtatatact gaactcagag aaaacactag caataacaat agtaaacatc
      181 aagaacttta tgataaaaat aatgattata gattctggct taaaataaac aacgcaaata
      241 tagactatcc tgtagtacaa ggatataaca aggatttcta cctaactcat gatttttata
      301 aaaattatct tcctgcaggt agtattttca tggattatag aaataacttt gaaaatgata
      361 agagtttaat agtctatggt cattatatga aaaataaaac tatgtttggt cagctagaaa
      421 gtagaaataa aatataaagg acaaacttat acttatgaaa ttttttcagt atatactgat
      481 tacttaattg gcagaaatta tttaagtata cactttaata ataatgatga gtttaaagat
      541 tctttgaatt atataaccga aagatcagta cacaaatctg atataaaggt agatatttca
      601 gataaaatta taactttgta tacttgtaac tatgaattta aagatgctcg tactatagtt
      661 catactaaat taatttcaaa aaaaagacta ataagtaggt ttgtttaaat acagatttac
      721 ctacagtctt taaatcatat aaggatctgt ttaaaaatgg caactaaaag agctaatggc
      781 gaggggtcta ttgttaaaaa tataagaaat ggtgttcaga taggatggcg cgcttctata
      841 agtattggtc gtgatgagaa ggacaaactt ataagaaaac aatttactgt aaaaacacaa
      901 aaagagttaa aagaaaaatt agatatttat aggacaaaga tgctcttagg ctctatagtc
      961 tcagctgata aaataacctt tgaagattgg ttttatactt ggttatttga ttatagagca
     1021 aaaaatttaa aacctaaatc ttttgaaaga tatgagggca tttatagaaa tcacaaaaaa
     1081 gatactgaac ttggaaaaat aaaattaata gatttaagag ctacacatat acaaagatat
     1141 tacaataaac ttattgatgt atataacaaa cctgcatcaa caattataac tctaaataca
     1201 agattaaagc cttgtctaac agaaacagaa aaacaaggtt atattcaaaa aaattattgt
     1261 aaaatgataa cactcccatc tgataacaag ataaaagagg tacaaatatt aagtattact
     1321 gaccaaaaaa aatttattga tagcattgat ggagataagt ttgaaatact ttttcttctt
     1381 gctttgagtg ctggtttaag attaggagag ttacttggtt tgaaatggag tgatattagt
     1441 tttgatgatg aatctttaac tgttagcatg actttgcaac gagttactga aataaataaa
     1501 gatggaacta gagaaaaaaa gtcattgaac agtatacaaa gactaaaaat tctataagaa
     1561 cagttcctat tccaagaaat gttttagtta aattaaaaag cataaaatac aacaaacaga
     1621 acaaagatta ttgttaggtg atgcttatat aaataataat tatgtaattt gtaatgatac
     1681 aggtcttgct ttaaacaata atagacctgg taaaatttta gattctttac taaaaaaatt
     1741 aaatatacct aaaattaaat ttcatgctct tagacataca tatgcaacac gattattcga
     1801 ggctggtgtc cctcctaaga ctgttcagac attaatgtgt cattatgact atggatatat
     1861 acacacgtta tgcacaatac caagcaggat gctgtggata aaataaatga tattttttaa
     1921 tgtaaatgaa gtgaaattaa atttattaac agtaattaaa atgctataat atgtatatta
     1981 tatttagatt aagaaaagtc atatatttaa atacatggct tttcttaata ttatttgtct
     2041 acaatatgac agtcctttta ttttgatgaa tctagtatct caccaaataa ttttctatac
     2101 tcttctgata aagatacatt tacaagttca caccatatat gctcttgaat atctatattt
     2161 tcaatacacc atactttata atggcactcc ttagttatat aaaaatcaat tttaatttca
     2221 ttggtagaat tatcttcttg cttggaacag tattctttaa atggatactg gttttttata
     2281 aaatctatat gtctaaatgc ttttctttta ggataatatt taccatgaat aaaagttgtt
     2341 ataatatctt tatttgattt aaaccctctg tctatatatg gaagttcttt tatttcaata
     2401 tgccaaaact cttcatcaag aatatcatca tatctctttt ttataaccat aagtagttta
     2461 ttataattat cattatctcc atgaacaata atatttttca acttttctag cttagtatta
     2521 gaaaaatcaa atgtacatcc tcgccatttt tggaatttta aaatagtact aacatatgca
     2581 tacttcaatg gtactgcaag acgttcatct aatcttaaat aaaaatttga tttattaaaa
     2641 tctatatttt gggacataat ttcaaaaact gcaggtataa gactatttat atttatttaa
     2701 aggagattta tcaataaaaa tgttatataa gtaatatttt ttatcatata taaaaccatt
     2761 ttgtttaaag tccaagtttt ctattttagt aagtttataa tcatttatat catacatatg
     2821 tttttcatat gaaattactg acttaatatt taaatcatca tatctaaatc tatccaacac
     2881 aaaacatgta acaacacaaa acaatagatc ttcatcaatg gtaatattag ttggaaattc
     2941 tttttcccca catgaaatac tatatatatc tgaatatgcc acatccctag gaaatgtata
     3001 ggaaagtatt tactgtgcta caaaaaaata atgtgttctt ttcttggggt cagtaatata
     3061 ttgaaatatt tccgattgag ttaaatttct ttctttaatt agagatttaa cttcattaca
     3121 aagttgttta atataataag gaacaccact ccaattcctc accttaagag atttccataa
     3181 aggctcatct aaatgaaaag tataataact tgtattatag taactatcta aatttttgat
     3241 atcttgtatc aatttttcca ttattgttat ccccctaaaa attttagtct aattgtattg
     3301 atttacataa gcttctagtt gtgcgctaat tgtgtgtctt tcaataatgc aaaagggatt
     3361 actcagtcta aatccaagta atcccaatca tttcatcatt cttttttcaa aattggtgga
     3421 gacgaatcgt cagaataata cccacgatta attttgttat tatatattaa tactactact
     3481 cattaactaa aatttgaatt aaatcaaata ctaatttgaa attttcaaaa gatatattct
     3541 tgaactcctc tactccattt aatatactat ctgcaaaatc attcttgctt agtgcaatat
     3601 tatcctcagg attcttaata tcacatacta cacaccccga atctataata gacttctttc
     3661 cacatttatt tttatcttga catattttat cccctaatat atgtaatcct agtttagaat
     3721 taaattcatc gcctaaaaat aatcttctac ctttgtagaa tgtttttatt tcttcatcag
     3781 aataataatg ttctatacac attattttat caatttcaac cctgttatcc ggcaatggca
     3841 ataccattga tattacatta ttaccccata atttataatt tgtattttca tcttttactt
     3901 ttcttagatc cctatcactc aagtctctat caaaaacaca tattacctta ttattgttcc
     3961 ttaatttact tgccgcttca caaatattta ttaaattatt atagcccatt tttatatcat
     4021 tttcatactc taaaaattca atatctaaat catgatataa tccttgctct tttaatttta
     4081 ataaaccagc tttcatatgt ttccaatctg tctttccttc tgtaattatt aatgtctttc
     4141 catttttact ttttaatata tcttgtatat cttttgaaaa taattcagtc ctcttataat
     4201 attcatatga tctttcaaat tccgaaaact cttccggtcc aatgttctct cctgttggca
     4261 tttcatatat cttaaatcca tcctctttaa atatatcttt catacctaat ataaataatg
     4321 gagaatgaga tgttattata aattgaacct ttggaaactt ttttattagt tttggtagta
     4381 cgtcgcattg tatactcgaa tgtagatgta aatcaatttc atctattata acaatacctt
     4441 ctatttcatt taattttata ctcttattaa tatctccata atctgaatat ctaattattg
     4501 ttgaaaacat gttaaataat gctgactctc ctgtagataa agcatctaat gtaggtataa
     4561 tcattgaatt ggtcgaccta tcaattatat taaatctgga tccacctgaa tttctccaat
     4621 ttaatccaaa cttaacatcc ttctttacta tttcagataa tatttcttcc acattttctc
     4681 ttgctatttt tagtaatctt atattattta agtcttgttc cgaattccat ccacctaggt
     4741 cactctgtat taaatcagct ctagaatcta caattatatc taaaagccat ttaatattag
     4801 tttcagttac attttgtacg attatgggtt tatttaattt accagatatt ttatcattta
     4861 ttgttataag attttctgag atgatatttt gattcatcca gtgaggtgtt tcatatctat
     4921 tcggaggaaa aaaacaaaaa gatttatttg caaattcttc ctcaaaatac tctttatttt
     4981 tagttatatc ttttatattt tttctattac cccagttatt atctaaattt aatagtccac
     5041 tagtcttttc tatgcattgc tcaaatgata agttccctaa tttttctata tattgaaaat
     5101 tttgttctcc atttacaaag ttgataaatg aaaaagaata atcacatcca acctttgtgt
     5161 ttttccaaga taaaacttta aaataaggtg ttgtaccatc tttatttgtt tctgttatat
     5221 cattgtacgg ctgttttgca aattcaaata atgaatcaac tatgttgctt aatacagttg
     5281 atttaccctt tccattttca cctacaaata taattggttt tggacttcca tcttcattga
     5341 atggaaaccc tatatctaag ttatctatag gtcctacatt ctccattatt attctgtcta
     5401 aaaacattta ctatcttccc ctttatatat ttatattaaa aaagtattga tatttatatc
     5461 aatacttttc gtggatttca atggtggaga cgaggggagt cgaacccctg tccgcaaatc
     5521 ttttccttgc tgcatctaca tgcttagcca acttcactaa tatctcacct cacatagcgc
     5581 caagtggcag gctctatgct tagccagtcc caattaatct actttaagtc gagacacccc
     5641 taaagtagtt ccctgcataa tgacgtccag tcttagtcgg caggaggact aagagggacg
     5701 agcagcaact aggctgctat cgcgaaatta tcgtctgcgt ttattttaaa gttccccagt
     5761 ttttacgtga cccagagtaa cacgacatgc agcctcaagt tccaaaccca cgtcgaaacc
     5821 tttacgcccc cttgagtaat ttatagtcca gtagactctt cgcaaacttt atcaccattt
     5881 ttattaaatt tttaatgtat atataatata atacttttaa gaataaaaat caatgttatg
     5941 ttctagataa taaagaattt tattctaaaa aatacagttt tatctagttt aatacttccc
     6001 cgtcatttct ctttctatac gtctttgtgc atctttctta gccatatctt gacgtttatc
     6061 atatagtttt ttaccttttg ctaatgcaat ctctacttta accttaccat tttttagata
     6121 caaactcagt ggtataagtg aatacccctt tatagtagtt gctccaataa gttttcttat
     6181 ttcatgctta tgaagcaata attttcttac tcttaaagaa tcaacattga atatatttcc
     6241 ttgctcatat ggacttatat gaacttgttt taaaaatact tcactattat ctacagatgc
     6301 aaatccatca cttaagttaa ctttaccttg tcttattgat tttacttctg tcccttttaa
     6361 ttcgattcca cattcatagg tttcttctat aaaatattca tgtcttgctc ttttgtttgt
     6421 tgctaatatt tttttaccag ccatataaat ctcaccttct ttgctagaaa tttcttcaat
     6481 aaatattaga aatttgctaa atatttattg tcaccaaatt atttgactaa cttgtatgat
     6541 aacatattta ggcattttag tcaatatttc actgatatat taagttattt aggttatata
     6601 taataaacaa tataaatata taaaaataat taaaaaacta tttgaaatat acttaatatt
     6661 aaactacatt taaccagtaa cactacttta taaacttcaa aacaaaaata ttacatatta
     6721 taaattttct at
//
LOCUS       DAMJVM010000138         6720 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.143, whole genome shotgun sequence.
ACCESSION   DAMJVM010000138 DAMJVM010000000
VERSION     DAMJVM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.143"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            173..1180
                     /locus_tag="PC523_003277"
     CDS             173..1180
                     /locus_tag="PC523_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2476763.1"
                     /translation="MIFTIIFYKKGRLCLLNLQSKWVNFLKPTYVSDEIKRKKLQVII
                     FFSITYGLSYSLGLLAICFNHLIDSEILAQFMMILPLSSVSIAKFYTEGRNNDKYNFY
                     SSVILFFIIYFFIFIIESLKLISINQFQLMYLMLILISSLCVIVYSYKITSLYIFKNF
                     KIGILLVFYFLFSQIIFGLIISDNQFDYKNFLNYILLPIISLVYIYPFLCEEYGWRSF
                     LQSILFDRFGKKIGIVMVGTCWSLWHLPLQFTLYSPQTPIIGAIAHLIYGIGLSIFLG
                     YVYMKTKNIWFCSIMHVLINSLGLIFNDSEIIINYHSIIQRLIFILLFYTPFLFTKEY
                     K"
     gene            1560..3086
                     /locus_tag="PC523_003278"
     CDS             1560..3086
                     /locus_tag="PC523_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2476764.1"
                     /translation="MNESYWFLNSNPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKNIKDEYRTCKKIGINCEYRETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMAAVLENKLADGMFISIDDPSITFRQY
                     NDGNENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKKVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            3114..3671
                     /locus_tag="PC523_003279"
     CDS             3114..3671
                     /locus_tag="PC523_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476765.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKLFLVIFLFMFSMGVASLWEIGEFLMDTFIGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSSI"
     gene            4193..5236
                     /locus_tag="PC523_003280"
     CDS             4193..5236
                     /locus_tag="PC523_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476766.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKSIDEEGVV
                     VNIDNEILTKVEVVNDDNEIQEKIIEEDWLISENTLKLDDKEAIINDKDIELCKKEVQ
                     VESEKIDLNKFEELDQNENYNLEKNVIEEKEVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHKQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKN
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYKFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDVLEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            5347..6489
                     /locus_tag="PC523_003281"
     CDS             5347..6489
                     /locus_tag="PC523_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2476767.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKIKKREVK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKNNYLQVRASHILIKTVDDKGKQVSNSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEIKSDLVSSLTSEKQSLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         2790 a          565 c         1092 g         2273 t
ORIGIN      
        1 tttttatata aattttaaac agaagaataa atttctaacg aaattggata tttcttctgt
       61 aaatacatat tcaaaaaaag aattataaga atttagtcag cagtcgttta agtaataagg
      121 actttataaa tatatagaaa tataatgata tgagtataag tagaaaaatt ttatgatttt
      181 tacaattatt ttttataaaa aagggaggtt atgtttgttg aatttacaat caaaatgggt
      241 aaacttttta aagccaacat atgtcagtga tgaaataaaa agaaaaaaac tacaagttat
      301 aatatttttt tctataactt atggtttatc ttattcacta ggtttattag caatatgttt
      361 taatcattta atagattcag aaattttagc tcaatttatg atgatattac cattatcttc
      421 agtttctatt gcaaaatttt acacagaggg aagaaataat gataaatata atttttattc
      481 atcagtaata ttatttttta ttatttactt ttttatattt attattgaat cattaaagtt
      541 aataagtatt aatcaatttc aattaatgta tcttatgtta attttaataa gtagtttatg
      601 tgttattgtt tattcttata aaataacaag tttatatatt tttaaaaatt ttaagatagg
      661 tattttgcta gttttttact ttttattttc tcaaattatt tttggattaa taataagtga
      721 taatcaattt gattataaga atttcttgaa ttatattctt cttcctataa tatcattagt
      781 atacatttat ccttttttat gtgaagagta tggttggaga tcttttttac aaagtatttt
      841 atttgataga tttggcaaaa aaataggtat tgtaatggta ggcacttgtt ggagtttatg
      901 gcacttacct ttacaattta cactatatag tcctcaaact ccaataattg gagctatagc
      961 ccatttgata tatggtattg gactttctat atttttaggt tatgtttata tgaaaactaa
     1021 gaacatatgg ttttgctcta taatgcatgt tctaattaat agcttaggat taattttcaa
     1081 tgattctgaa ataattatta attaccatag tattatacaa agattgatat ttatactatt
     1141 attttatact ccttttctat ttacaaaaga atataagtaa gaattacaat taatttatat
     1201 aaaataaatt tttaagtaaa tatttttata gagagattgt tatattacat atcagaaaaa
     1261 tattaaacga actaaatcaa taaaaaagtt ggtggctaac aatttataat tgttagcaca
     1321 acatcatctt atgtattgaa atctaaaata ttctcatttt tacttagcat aaatactatt
     1381 aattaagaaa ataatataga aattataaat atatataagt ttagtctggt gtataaaaca
     1441 agaaaactaa gtgattttta atttagcttt ttattaagct tattttttat actatttata
     1501 ataaataaac tccatgatgg gaaaactatt actatttaat atgtaaagga gtattttaaa
     1561 tgaacgaatc atattggttt ttaaactcaa atccaaaaga atataacaaa ttaggtgaaa
     1621 atataaaaac agattgtctt atagtaggtg gtggaataac tggattgaca actgcatatt
     1681 tattagcaaa agaaggtaaa aaagtagtgc ttgttgaggc tgataaaata ggatatggaa
     1741 cttctggaag gaatactggt aaggtaactt gtcagcatga catattttac tctaagatag
     1801 agaaaaaata tggattagat aaagcaaaat catattataa tgcaaataat gaagctttaa
     1861 atttagttga acaaataata gaagaaaata atataaaatg tgattttaaa agggaaactt
     1921 cttttgtatt tacagaaaaa gaggatacta taaagaatat taaagatgaa tatcgtacat
     1981 gtaaaaaaat aggaataaat tgtgagtatc gtgaaactat agaaaatatt ccattagata
     2041 taaagggggc tattagtttt actaatcaag gtcaatttaa tccaaagaag tatattgatg
     2101 gattggcaaa agcagcagta aatttagggc taaaaatata tgaaaataca ccagtagtag
     2161 accttgagaa aggcaaaata tgtagggtta aaacaagaga ggataatata atagaagctg
     2221 agaatgttat aatttcatcc catagtccat ggtatgatgg attgaatttt tattttgcaa
     2281 aagaatatgc agagcgtgct tatttaatgg ctgcagtttt ggaaaataaa ttagcagatg
     2341 gtatgtttat aagtatagat gacccatcaa taacttttag acaatataat gatggaaatg
     2401 aaaatttact gatttttgga ggaggagacc ataaagtcgg tcagggagga actgagaaag
     2461 aaatatttga tgatttagag cattatggga aaaaagtctt taaggtaaaa gattttaagg
     2521 ggaaatggtc tgctcaagat aatatgagtt ttgacaatgt tccttatatt ggttatatca
     2581 acaaaagaga agacaatatt tatgtagcta cagggttctc taaatggggg attactaatg
     2641 gaactgctgc tggaataata ataaaagatt tgataataaa taataattct gattataaag
     2701 atacttttaa tccatccaga ttaggtagct acttttcaaa agattttata aaggaaaatg
     2761 caaacgtagc aataaattat gtaagtggaa aattaaaaat aggtagtgga gatatgccta
     2821 aaaataatgg agaaggaaag attgttaata ttgatggaaa aagatatggt gtatataagg
     2881 atgataatgg agatttttat attgtagaca cgacatgtac tcatttagga tgtgaactta
     2941 attttaacag tgaagaaaaa acatgggact gtccatgtca tggttctaga tttgactata
     3001 agggaaatat acttgaagga ccagctctta agccattaaa actttatgga catggtgata
     3061 atgatgttaa tcctaagtta ttataaaatt atttttagga aggtaaagtg tatatgaata
     3121 aacttttaaa gccaataact atactattgt atatagttta tattttatct gctgtatttt
     3181 ttgtagcaaa caaagaatat ggaagtctag gtctagtagc aattagttta gttggtactt
     3241 tcattttgag ttttataaat aagcaaactg aaagattatt gagtaaagaa ttatatataa
     3301 tgcttgcttt atttataatg ttttcatcat tattaggtac ttgttttaat ttttatgata
     3361 taaattatta tgatgatttt ttgcatttat ggtcaggtat aatatcttgt attgtggctt
     3421 tttctataat taaatacttt tatacacaaa gagagataag taaaatgagt aaattgtttt
     3481 tagttatatt tctatttatg tttagtatgg gagtagcgag tttatgggaa ataggtgaat
     3541 ttttaatgga cacttttatt gggactacta cacaggctgg aggattagaa gatactatga
     3601 ttgatatggt agatgcttta ataggtacaa ttataactat tccttttata gtaaaaaaat
     3661 ctagtatata gttttaaatt ttttatatta gttataatgg atttaattaa tcatgttgag
     3721 tttcaaggtc tatatgatta attaaatcca tttttgttat ttataattat tattgaatat
     3781 atttttacat atgagatata cggtgataga tagaaattgt tggattatgt aaataattaa
     3841 tatataatac cattatgggc aataaataca aatcaaatga taatatagtc tatttatgta
     3901 aatgttatat tgtatcctaa gtataatagt aagttttttg ataatggtgt agaaagtaaa
     3961 aatctactag tctaaattga tgtatggatt agtttaggaa cagtaattgc atatcttaaa
     4021 tttgatgtca tgttgagcta ctagaaatag gtcatctttt tcacttgaat atcttttctt
     4081 taaacatggg gagtatcata tcaaaaacat atgaaaatta agtgatttat aagaacagta
     4141 atattttata tgatagtata gaatagtaat aaagtattag gaggaacatt gagtgttaaa
     4201 aaagtggttt ggtattgtga aaaaaagaca aaaaagtgag tcagtaaaag aagaaggtga
     4261 agtaatatta aaaaatgaaa aaatattatc tgaagaaaag tcgatagatg aagaaggagt
     4321 tgtggttaat attgataatg aaatattaac taaagtagaa gtagtaaatg atgataatga
     4381 aatacaagaa aaaataatag aagaagattg gttaattagt gagaatacct taaaattaga
     4441 tgataaagaa gcaattatta atgataaaga catagaatta tgtaaaaaag aagttcaagt
     4501 tgaaagtgaa aaaatagatt taaataagtt tgaagaactt gaccaaaatg aaaattataa
     4561 tctagaaaaa aatgttattg aagaaaaaga agtaagtgaa tgtttgacag aagaagattt
     4621 agagtacata aaagaaatta aaataaaaag aggaaaaagt ataaaagcta taaacttgta
     4681 tactaaagaa gagtgggttt ttgacactca taaacagtgt agtaaaaaac tcaaagttcc
     4741 attagggtat atacgagaaa atttaaaata tggatatatg gattactttg gagatgcaat
     4801 aaattattta agtgaagtat taaatataga tgaatactgc aaaaatgaat ggagctatct
     4861 cgataatagc aaatctccat ctgagatatt taatattcta aacaataaaa tatttagcat
     4921 aaggctttct aatgaaaaaa gaaatgaaat tttgacaaat gataaaattg aagcattaaa
     4981 aatgaattat aaatttgaat gtattgatga agaatatgat gaatatttta aaaaatataa
     5041 gtctataatc aaaagaggtg gaaagaaaaa agttgaatta gtaaataaaa aaggtgacgt
     5101 tttagaaata tttaagtctt tagaagaatg tgccatttat ttgcaaaagg aaaagaatga
     5161 agttatacaa atgttaaaat atggagatac aaaagtagga aggaacttta taaggtatag
     5221 tttgagaagt atttaatttc attttaaatt cataagagtt tcatttctga ttcacacatt
     5281 taatattatt ttaatgtaaa ataataaagg tgaagtattg cacaattaaa attaaggagg
     5341 gtattaatgt ctgaagaaaa aaatccaaaa gaaaatacat tagatgaatc taaaaattca
     5401 gataacgtaa attctaaaaa agatgatgtg tcagatgaaa aaaatactga taacaaaatt
     5461 aaaaaaaggg aagtaaagct aaacaacaaa acacttaaag ctattgtagc aattgttgca
     5521 gtaggagcta tatgtttagg tggaggctat gtttttggca aagaagctgg aagaaaattg
     5581 cctgctactt caaaacacta taatagcagt gaggtagtag ctactattgg ggataaaaaa
     5641 ataacaggtg aaatgttaag aaagagaatg gagccattat tttatttaaa tgcaaaaact
     5701 actatgactg atgatgaaat acgttcatat gaatcaaata tgattgaaac tatgattaca
     5761 tctgatatat tatcctctga agcagcaaaa gagaaaataa gtataactaa tgaccaacta
     5821 gatgctcagt ataataattt aatgagtagt atagaatcag caatgggaat gactaaagaa
     5881 gagtttttaa aacagtttga tttgactgaa aaatatataa aagatgatat gaaaaaagaa
     5941 ctggtagcat caaaatactt aaatgaaaaa tcaaaagtta ctgataaaga agcagagaac
     6001 tattacaata aaaacaagaa caattattta caagttagag cttcacatat tttaataaaa
     6061 actgttgatg ataaaggtaa acaagttagt aatagtaaaa aagctgaact taagaaagaa
     6121 gctgaagaaa tacttaaaaa agcccaagct ggggaagatt ttgctactct agctaaaaaa
     6181 tactctgaag atagtagtgc agaatcaggc ggagatttag gtttctttgg taaaggtcag
     6241 atggtggaat cttttgaaaa agcagcattt gcattgaaga agggagaagt atctaacaaa
     6301 cttgtagaaa gtgattatgg atatcatata attaagaaaa ctgatgaaaa atatcaacca
     6361 tttgaagaga taaaatcaga tttagtaagc agtttaacat cagaaaaaca aagcctactt
     6421 atacaaaatc taaaagaaaa atataatgtt aaaatcacag ataaatatca aatgaaagct
     6481 aaagaataga taaagtcaat ttataaaaaa aaagaaaaat ttactgtatg gataaaagct
     6541 atttttgctc tcaatataga gagtctaaaa tagcttttat tatttttgaa taaaatctat
     6601 ttacatatat gaagtgacaa agaaataaca tgcaaaaaaa tattaacaag aaatatcctt
     6661 aatttgaatt attttagata aaacatttat aaaaatgaaa aaataaacaa aaaataattg
//
LOCUS       DAMJVM010000139         6475 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.144, whole genome shotgun sequence.
ACCESSION   DAMJVM010000139 DAMJVM010000000
VERSION     DAMJVM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6475
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.144"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            114..845
                     /locus_tag="PC523_003282"
     CDS             114..845
                     /locus_tag="PC523_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2476768.1"
                     /translation="MTRKKMYILISIAICDDESYMLNHLKENIELYMKQNNLSYNIEL
                     FDKAEDLLCNHEKFNIVFLDIQMEDINGMQTARELRVYGNDCFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLEDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETIDYYETIENLERSLPTNFFRCHRSYIINLKYVYGYESGNAVMESGD
                     RIPISRLRQQIFSKVMLEFMKNRRR"
     gene            847..2205
                     /locus_tag="PC523_003283"
     CDS             847..2205
                     /locus_tag="PC523_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2476769.1"
                     /translation="MINLFASDLFLKYSFLFSFTIYRILMVIVQYYFLINFTNVNSKF
                     WCFILYSIISIFLIIISTYFNLYNLFSSQILFFLMTFLFVKYIIKQSTVLSTVSTLLV
                     YLIEQLVYGFTAPLNYLIFTSDINTDIYKFDINMALSSLITIIIAGFVYWYSSKKFNI
                     KVMNFDKYLAILIIPLLLIILFMQSFKHSSNINIDTSLGTVKLLLNTSITEEIQAFLF
                     SIIGTICVFFSLFTFKKLIQSLEDDKERAIMNQQIHAQKNYIEEAKSRLSQTISFRHD
                     FNNHLAIVNGLLKKGQILKAQDYLNKLEKVTSDLSFSCNTRNVVIDALFNNKLSIAKQ
                     LGIEVDCEVIIPKSTNMNDFDLCIVFANAIDNAIKACRVVDKQSRYLKISTKLEGGFF
                     MIEIKNSYNPINNYTSGSCIGLSNIKEIAKKYQGAISIDKTSDYFQINILLVISLHCA
                     GI"
     gene            2244..2486
                     /locus_tag="PC523_003284"
     CDS             2244..2486
                     /locus_tag="PC523_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476770.1"
                     /translation="MYLNKESLIYRNEEVTKMKIGNINGQAPITNGLDSCQSEVQGLE
                     QKKKALQDEIEKVRSDKNIDSETAKLKIEDLRKQIE"
     gene            complement(2942..3166)
                     /locus_tag="PC523_003285"
     CDS             complement(2942..3166)
                     /locus_tag="PC523_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2476771.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(3263..4516)
                     /locus_tag="PC523_003286"
     CDS             complement(3263..4516)
                     /locus_tag="PC523_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HDF2476772.1"
                     /translation="MITLTFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYNLKPSSNIDLSVPTYNRNLNRFGSKFFRNK
                     NMVKNNNFSQTITPSVFLEQVLLIFICINTGEIISRCFYIMFNILLPSVVTCMFSAVL
                     FRNLNDKISILELNFKLIDFLKEISLGIFLTLSLMNIDLFELSTLLPPILLIVIFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGNSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            4776..5765
                     /locus_tag="PC523_003287"
     CDS             4776..5765
                     /locus_tag="PC523_003287"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDF2476773.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDAFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDNKKAYNLVKDKKILLELCPTSNVQTKTVDSYETHPFYTFYKDNLHVSINT
                     DNRTVSDISLSSELNVIFDTFKLGIEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            5837..6403
                     /locus_tag="PC523_003288"
                     /pseudo
     CDS             5837..6403
                     /locus_tag="PC523_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
BASE COUNT         2621 a          704 c          936 g         2214 t
ORIGIN      
        1 ttaaattaga tatactatga aaagtgagct tattacattt tggaacaaat attcaacata
       61 ctagaacata ttaattgaac agaaaaaatt aactttatat aataattaga ataatgacaa
      121 gaaagaagat gtacatattg atttcaattg ctatttgtga tgacgaatcg tatatgctaa
      181 atcatttaaa agaaaatata gaattatata tgaagcagaa taacttatca tataatatag
      241 agctttttga taaagcagaa gatttactat gcaatcatga aaagtttaat attgtatttt
      301 tagatataca gatggaagat attaatggaa tgcagacagc tagagaatta agggtatatg
      361 gtaatgattg ttttataatt ttcgttacag ttttaaatga atttgtattt gatgcttttg
      421 aagtagatgc tataaattat ttgataaaac ctctatctaa tgagaaacta tataaaactt
      481 tggataaaat agttacaaaa cttgaggata atgaaagtaa ttatatcaca atacaaaaag
      541 gttattcata ttggaaatta aaacttgctg atattttata ttgtgaagtt ataaaaagaa
      601 agatttatgt tcatttaaag aaagagacta tagattatta tgagactatt gaaaatttag
      661 agagaagttt accaacaaat ttttttaggt gtcatcgtag ttatataatc aatttaaaat
      721 acgtatatgg atatgaaagt ggtaatgcag ttatggaaag tggagataga atacctattt
      781 ctagactacg ccaacaaata ttttcaaaag ttatgcttga gttcatgaaa aataggagga
      841 gataagatga ttaatttatt tgcttctgat ttatttttaa agtacagttt tttattttct
      901 tttactatat atagaatttt gatggtaatc gtgcaatact attttctaat caattttaca
      961 aatgttaatt caaaattttg gtgttttatt ttatattcga taatttcaat ttttttaatc
     1021 atcataagta catattttaa tttatataat ttattttctt cacagattct attttttctt
     1081 atgacttttt tatttgtaaa gtacataata aaacaatcta ctgttttatc tacagtatca
     1141 accttattag tatatttaat tgaacaatta gtatatggat ttacagctcc attaaattac
     1201 ttgatattta ctagtgatat aaatacagat atttataaat ttgatataaa tatggcacta
     1261 tcttctttaa ttaccattat aatagctgga tttgtatatt ggtatagttc taaaaagttc
     1321 aatattaaag ttatgaactt tgataaatac ctagcaattt tgataattcc attgttgctt
     1381 ataatattat ttatgcaatc atttaaacat agttcaaata taaatataga tacaagtctt
     1441 ggtactgtaa agttgctatt aaatacttct ataactgaag aaattcaagc ttttcttttt
     1501 tctataattg gaactatatg tgtatttttt tctttattta cctttaagaa actaattcaa
     1561 tctctcgaag atgacaagga gagggcaatt atgaatcaac agattcatgc tcaaaaaaac
     1621 tatattgaag aagcgaaatc acgattatca caaactatat cctttaggca tgattttaat
     1681 aatcatttag ctattgtcaa tggattattg aaaaaaggtc aaattttaaa agcacaagat
     1741 tatttaaata agcttgaaaa agtaacaagt gacctatcat tttcttgtaa tacacggaat
     1801 gttgttattg atgctctttt taacaataaa ctaagtatag caaaacaatt aggcatagag
     1861 gtggattgtg aagtaattat acccaaaagc actaatatga acgattttga cctatgtata
     1921 gtatttgcta atgctattga caatgcaatt aaagcttgca gagttgtaga taaacaaagt
     1981 aggtatttaa agatttctac taaattagag ggtggatttt ttatgataga aataaaaaat
     2041 agctataatc caataaataa ttatacaagt ggttcatgta tagggctttc aaatataaaa
     2101 gaaattgcta agaaatatca aggggctata agtatagata agacatcaga ttattttcaa
     2161 ataaatatat tgttagttat ttcattacat tgtgcaggta tttgagaaca atttcattga
     2221 aaatagtgtt tataagctat attatgtatt tgaataaaga aagtttgata tatagaaatg
     2281 aggaggttac taaaatgaaa attggaaata taaatggtca agcaccaatt acaaatggct
     2341 tagatagttg tcaaagtgag gtacaaggct tagaacaaaa gaaaaaagca ttacaggatg
     2401 aaatagagaa agtaagaagt gataaaaata ttgatagtga aacggccaaa ttaaaaatag
     2461 aagatttaag aaaacagata gaatagattg aaagtaaaat tcaagagtta aaaggagata
     2521 aagcacaaga aacaaataaa gaagaaaata acgtttcaga agtaaataat aatctattag
     2581 acagaaaaaa agaaccttct aataatgaaa atgtaaaaca attatatgat gtttttgaaa
     2641 gaaacaatgc agacgaacaa tgggataata cttatcatgt tgttcaagat gataaaaact
     2701 taaaagtaaa atttaatagg cctatatagt ttatttataa aaatagttga tattttaatt
     2761 atcaacattt tttagtagaa tacacaattt atttacagtt agtacattta taggtggttt
     2821 tataactatt atttacaagt ctatcttgat aacttttaca tggtatataa tgcttaaata
     2881 tttattcaca tttatctata cataaacaaa aaaagagatt ctaaaatctc ttttttattt
     2941 attaattatt ttttaaattt atattttttg catcagattt tctaatagct aagtcaaacc
     3001 ctctaaatct aatcactata gggtcattaa gaggagctat ctttttcact tcaacttcag
     3061 tgccatctat taatcctaag gctagaagtc tcttcataag tttttcattt ccagctatat
     3121 tgtctataat tcccttttgt ccaagtttca agttgtaaac agtcattatt aacacctcct
     3181 aatgaaaatc attatcaaac tttatattta taatatataa caaaaagagc aaattgtcaa
     3241 tatataacaa aaaataaaca tactaagtta gtatgtttat aaaaaaaagt atgcatggga
     3301 tactaatagc atctagcaaa aaaccactta ccaatggtac aaccaaaaat gcttttggcg
     3361 aatttccata tttttgtgtc aaagaactca tatttgccaa agcgtttgga gtagctccta
     3421 ttccatgtcc aatcaaacca cttattatga tggcagaatc aaaatctttt ccaagtactc
     3481 taaagcaaat aaatattgaa aataggatta taaagattac ctggaaaatt actattaaga
     3541 gaattggagg taataatgta gatagttcaa acaagtctat attcataagt gataaagtta
     3601 aaaaaattcc taatgaaatt tcttttaaaa agtctataag cttaaaattt aattctagta
     3661 tacttatctt gtcatttaaa tttctaaaaa gaacagccga aaacatacaa gttacaacag
     3721 aaggtaatag gatattaaac attatataga agcatctact tataatttct cctgtattta
     3781 tgcaaataaa tatgagaaga acctgttcca aaaacacaga tggtgttatt gtttgagaga
     3841 agttattatt ttttaccata ttcttatttc taaaaaattt acttccaaat ctatttagat
     3901 ttctattata tgtaggaacg gataagtcta tatttgatga tggttttaaa ttatatttat
     3961 ttattagaaa tttagctaca ggacatccca atataccacc cataataagt cctattgttg
     4021 cagcagataa gccaactcca actgctcctt ctataccaag attttctatt gtaagtccaa
     4081 atgcagcaga ataaccatga ccaccttcca tagatacatt tccacacata agccctaaaa
     4141 gaggttcaag ttttataatc tttgatattg taatagctaa gatattttga caaaatgcca
     4201 atactccaca caaaatccaa tatctaaata atacactacc acctttcttt ataagattta
     4261 tacttatttc aagtcctata gttgtaaaaa agaaggttat aaaatttggc attaaatttc
     4321 cacttataga tatatctgct atattaaaat atttaaaaaa taaatttaga aaacaaaata
     4381 atagacctcc tattactggt gcaggtatac agaaattgtt taaaaagtta actttattct
     4441 tcaataaatt accaattaaa aagaagaata tagatagtgt caaggtttgc actatatcaa
     4501 aagttaaggt aatcaaaata agtcctcctt tgtaatttaa agttttaatt atataactta
     4561 aatttttatg tattgaaatt ttattatcaa atgtatatta ataaatgtat attaaaaaga
     4621 atcagtagat ataattagat agtcataaat taaattaaaa tattttaatg agatgtgact
     4681 tttttagaat gaaaaatcgt agtatattga tataatagaa ataagactta aaaatctgtt
     4741 gatagtatta tttataagta cagtaggagg ataaaatgtt tgaaaattta cctaagatag
     4801 atttacattg ccacttagat ggaagtgtta gagtagaaac catgcttgat atagctatta
     4861 aagagaaaat agatttacct tcaaataata tggatgaaat aaaaaaatta gcaaaggttt
     4921 cttttaattg tacttcactt gatgaatatt tagaaaaatt tgatttacct ttaaaagtaa
     4981 tgcaatctaa ggaaaattta aaaagaatta cttttgaact tttggaagat gcaagtagag
     5041 aaaatgtaaa atatattgag attagatttg caccattact tcacacccaa aaaggtatga
     5101 gtgttaaaaa tataattgag ggtataattg aaggaattag agaagctgaa agtatttatg
     5161 atataaaagg caatttaata ttaggttgta tgagaacaat gacctcaaaa gaggcattat
     5221 tagtaattga agaagggaaa tcatttgtaa ataaaggtgt tgtagcagta gacttatgtg
     5281 gtccagagaa agagggattt tgtaaggaat ataaagatgc tttcaaatta gcaagagaat
     5341 atggttataa agttacaata catgcaggag aagctgcaag tggagaaaat gtattggatg
     5401 ctataaatat tttaaaggcg gataggattg gtcatggggt aaaaattaaa gacaataaaa
     5461 aggcttataa tttagttaaa gataaaaaaa tattgcttga attatgtcca actagtaatg
     5521 tacaaactaa gacagtagat tcttatgaaa cacatccatt ttatactttt tataaagata
     5581 atttacatgt aagtataaat actgataata gaactgtatc agatataagt ttaagttcag
     5641 aactaaatgt tatatttgat acttttaaac taggaataga agactataaa ataatttata
     5701 gaaatgcagt agaagctagt tttgcagaca aagaaactaa agagtatttg aatagtttaa
     5761 tttaatagat aaaaatatat aaactttaaa ttaagctatt aaattgacaa aaatacattg
     5821 tattaggaga aaaaatgtga aagattataa agatgttgtt tatattaaac tagtcaaaaa
     5881 agaacttgaa gaatggaaga aaaatatatt aaagaaacct tctatatttg acaaaacatc
     5941 aaaggggata cagaataagt ttaatggagt attgccagat aaatatcatg aaataattac
     6001 ttcttctata aaagtaatga caaaaactgt attgtttgga tataaatatg ccacaaagcc
     6061 accacttaga aagatgtcat tagaagaaag agaaaaatta gttttagaaa aagctaaaac
     6121 atacaagacc acagcaatga ttgaaggagc aggaacaggg gcaggaggaa tcctcttagg
     6181 tttatctgat tttcctttac ttttaggtat taaagtaaaa tttctatatg atgtagcttc
     6241 aatatatgga tatgatgtta aagattatag agaaaggatt tatatattaa acatattaca
     6301 gttggctttt tctagccaac agcatataaa taaaactttt aaattgataa ataactggga
     6361 tgaatatgta agtagtctac cagaagacat caatgatttt gattaaaaaa tagctacgca
     6421 cacttgtgac tttagtcatg agttagtagc taaaatagtc agcatatagg gaaac
//
LOCUS       DAMJVM010000140         6427 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.145, whole genome shotgun sequence.
ACCESSION   DAMJVM010000140 DAMJVM010000000
VERSION     DAMJVM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6427
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.145"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            301..1245
                     /locus_tag="PC523_003289"
     CDS             301..1245
                     /locus_tag="PC523_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HDF2476774.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFITINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGESSVEADREVVKLALKILEKYNNKFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNIHELKVYIEELKIKCEVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1267..1893
                     /locus_tag="PC523_003290"
     CDS             1267..1893
                     /locus_tag="PC523_003290"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDF2476775.1"
                     /translation="MDYLTIALAKGRIEGESFKKLKKMGLGNSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1927..2976
                     /gene="hisC"
                     /locus_tag="PC523_003291"
     CDS             1927..2976
                     /gene="hisC"
                     /locus_tag="PC523_003291"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDF2476776.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEIADS
                     NIDLNLYPEDSYSDLKESIIDYINISGVSKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVIDIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKRKDIKKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEERKILYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     AMFIGAIRDILKKEK"
     gene            2979..3557
                     /gene="hisB"
                     /locus_tag="PC523_003292"
     CDS             2979..3557
                     /gene="hisB"
                     /locus_tag="PC523_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HDF2476777.1"
                     /translation="MRIWNVERNTLETQISVELNIDGSGKAEIDTGIGFLDHMLTLMA
                     FHGKFDLKVICKGDTCVDDHHSVEDIGIAIGESFKNALGDKKGIRRYSNIYIPMDESL
                     SMVSIDISNRPYLVFNAKFDIQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3559..4164
                     /gene="hisH"
                     /locus_tag="PC523_003293"
     CDS             3559..4164
                     /gene="hisH"
                     /locus_tag="PC523_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDF2476778.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSNIDEIKQADSLILP
                     GVGAFRDSISALEKLGLIPIIKEHVSNGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELVAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4161..4883
                     /gene="hisA"
                     /locus_tag="PC523_003294"
     CDS             4161..4883
                     /gene="hisA"
                     /locus_tag="PC523_003294"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDF2476779.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKINVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIDEIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAVENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEIVSDVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFNIYEKIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4865..5623
                     /gene="hisF"
                     /locus_tag="PC523_003295"
     CDS             4865..5623
                     /gene="hisF"
                     /locus_tag="PC523_003295"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDF2476780.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERTTSLEFVTKVAENISIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKMEDFYQAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKNMGVEVRL"
     gene            5651..6319
                     /locus_tag="PC523_003296"
     CDS             5651..6319
                     /locus_tag="PC523_003296"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDF2476781.1"
                     /translation="MNNKCNDIYTDEVDEFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKEAIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDTAKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2617 a          535 c         1270 g         2005 t
ORIGIN      
        1 caaaaaactg taaatttaga atattaataa aaattattta ggaggaaatt atcatggtga
       61 gattaaatat agtaaaatta aatacaaaat taaatcatca ccatcatcat agataaaagg
      121 gattgtaata tgaaattaat tcatagacac aagccctgtt tttagtgtaa ttggtcttgt
      181 atctatgatg ggaagtaatt ttaagagaaa attatagcca tataggtata aagcctatat
      241 ggctttttat tgttttaaat ttgcaaatat aagagtaaat ttaatgaaga gggtgagaat
      301 gtgaagtttt tgaggattga agatgaggtt atttattcta aaaaaagata taaattaaaa
      361 agagaaatag ataaactttt tttggatgaa gaatattttc aaatagaacc acaattattt
      421 gaagagtatg atgaatttat aactataaat aataaaattc cagaagaatc tatggtaaag
      481 gttgtaaatg gaaaagtaat ggtacttaga gctgatataa ctacaagtat aataaaaagt
      541 cttgtaccta gatgggaaga tggtttaaag cttaagcttt tttataattc atcaatatat
      601 aaaaataaaa atacagttgg aataaaagag ataagacaaa taggttgcga gtatttagga
      661 gaatcttctg tagaagcaga tagagaagta gtgaaattag ctcttaagat acttgaaaaa
      721 tacaataata aatttatttt agaggttggg agtagcaagt atattcatgg tttgttagaa
      781 gaattaaatt taaataagaa ttgtgaaaat caaattaaaa atttactata tactaagaac
      841 attcacgaat taaaagtata tattgaagag ctaaaaatta agtgtgaagt aaaagagtta
      901 ttatcaaata ttttaagttt acaaggaaat ttatacaacg ttattgaaaa atcaaacaag
      961 ttctattgta ataataagat gaagcaagct cttgaagaat taaagcaagt gaataattta
     1021 attgaggaat gtaatttttt agataaggcc agatttgact tatcaatgat tacgatgctt
     1081 gactattatg aaggaattat gtttagagga tactatccaa attcatataa agaaattctt
     1141 agtggtggaa gatacgattc tttaactaaa gaatatggaa aagaaattcc agccattgga
     1201 tttacactaa gtgttgatga attaatgaag tatgtatata agtaaagctg agaaggagga
     1261 attgatttgg attatttgac tatagcacta gcaaaaggac gaattgaagg ggaatcattt
     1321 aaaaaattaa aaaaaatggg tttaggaaat agtatagaca cagatacaag aaaactaatt
     1381 tttaaagacg aagaaaataa aatcatatat atacatgtaa agccatcaga tgtagttaca
     1441 tatgttgaaa aaggagttgc tgaccttggt atagcaggta aggatactat tttagagaat
     1501 gagacggatg tatatgaaat atatgattta ggttttggaa aatgtaaatt tgctgtggca
     1561 ggattaaagg gagatagcat atatagagaa gatgaatatc taaaagtggc aacaaagtat
     1621 cccaatatag cgaaaaagta ttttaaagag aaaggtcaaa aaatagaaat aataaaatta
     1681 aatggttctg tagaactagc tccaatagta gggttgtcag atgtaattgt agatattgtt
     1741 gagactggca atacgcttaa agcaaatggt cttgaaatat tagaagatat atgtaatata
     1801 agcgctagaa ttatatcaaa tagagctagc tatagattta aatacgaaca aattcagaat
     1861 ataattcgtt tgtttgaaga acttgataat taaaatataa tggaacttta caaggagggg
     1921 tttaagttga gagaaaaaga aagtataaga gagttaagag gatatgaacc aaatcacgta
     1981 aactgtaaag taaaacttga tgcaaatgaa ggaagtaaga ggttgtttaa ataccttatt
     2041 aaagagattg cagatagtaa tatagattta aatttatatc ctgaagattc ttatagtgat
     2101 ttgaaagaat ctataataga ttacataaat atttctggag taagtaagaa aaatcttcta
     2161 gtaggaaacg gttcaagtga aataattgat ttaataatac atacttttgt agataaagat
     2221 gaagtaatac tgagtttctc tccaagtttt agtatgtatt ctatatatag tcaaataaat
     2281 ggttctaaat ttataggtgt tgaaagtgat gaaaatttag ttatagatat agataatgta
     2341 attgaaaaag taaaggaaaa taatcctaaa atagtaattg tatgcaatcc taataatcca
     2401 actggaacaa tactaaagag gaaagatata aaaaagcttt tagattcaac taatagtcta
     2461 gtggttttag atgaagcata tatggatttt ggagaagaaa gtatgttaag tgatgttttt
     2521 aagtacgata atctaatagt tcttagaact ttatcaaagg cgtttggatt ggctggaata
     2581 agaacaggat atatgttatc taatagcagt ttaataaatt ctgttgaaaa agtaagacct
     2641 ccatataatt taaattcatt gtcagacttt attgctacaa gagcacttag aaataaagat
     2701 gtggtaaagg cttatataaa ggaagtaaaa gaggaaagaa aaattttata tgaagagatg
     2761 attggtatgg gaattaaggc atataaatca caagcgaatt ttatattgtt ttactctgaa
     2821 atcgaaaatt tatcacaaaa acttattgat agaggagttt taataagaaa atttggtgga
     2881 aagttagaaa attattatag ggttacgatt ggtgataaag aagaaaatgc tatgtttatt
     2941 ggagctataa gagatatttt gaaaaaggag aaataggtat gagaatttgg aatgtagaaa
     3001 gaaatactct tgaaacacag atttcagtag aattaaatat tgatggctct ggtaaagctg
     3061 aaatagatac tggaatagga tttttagacc atatgcttac cctaatggca tttcatggca
     3121 agttcgattt aaaagtgatt tgtaagggtg atacatgtgt agacgaccat catagtgtag
     3181 aagatatagg tatagccata ggtgaatcat ttaaaaatgc tctaggtgat aaaaaaggaa
     3241 taagaagata ttctaatatc tatataccta tggatgaatc tctatcaatg gtatcaatag
     3301 atataagcaa cagaccttat cttgtattta atgctaaatt tgatatacaa atgattggta
     3361 gtatgagcac tcaatgcttt aaagaatttt ttagagcttt tgtaaatgaa tctagagtaa
     3421 cattgcatat caatctctta tatggggaaa atgaccatca taagatagaa agtattttta
     3481 aggcatttgc aagagcttta aaagaaggta gtgaaatagt ttctaatgag atagcatctt
     3541 caaagggggt tttgtagaat gaatataata gtagattatg gtcttggaaa tatagattct
     3601 gtgtctagag gtttcagaaa agctgggata gaaactaaaa tttctagtaa tatagatgaa
     3661 ataaaacagg ctgattcact tatacttcca ggtgttggag cttttagaga ttctataagt
     3721 gccttagaaa agctaggatt gataccaata ataaaagagc atgtttctaa tgggaaattt
     3781 atgataggta tatgtctagg aatgcaatta ctttatgaaa aaagttatga gtatggagaa
     3841 tatgaagggt taggacttat aaaaggaagt attgataaac tggatataag tttaaaagta
     3901 ccacatatgg gatggaataa tttaaaattt aataaggcaa atgatgatat acttaaatat
     3961 ataaacgaag atgattatgt atactttgtt cactcatatt atgcaaattc atcaaatgaa
     4021 gaactagttg cattttcaga atatgagaag aaaattcctg ctattgttag aaaaggcaat
     4081 gtatatggta tacagtttca ccctgaaaaa agtggagaag taggtttaaa tatattaaga
     4141 gcatatgggg agatgataaa atgataatat ttccagcaat cgatataaaa gataataagt
     4201 gtgtgagact cacacaagga gaatttgaca aaataaatgt atattatgat aatccgttag
     4261 aagtagcata taaatggaaa aatgaaggag cagaatatat acatatagta gatttaaatg
     4321 gtgctagaag tgaatttggt gtaaatacaa aaataattga tgaaatagca aacaatatag
     4381 atatacctat tcaagttggt ggtggagtta gagataagga aaaagttaaa agtttaataa
     4441 atgctggtgt tactagagta atattaggta gtatagcggt tgaaaatttg aatttggttg
     4501 aagaattggt caatgagtat aaagagaaaa tagtggtttc tatagatgct aaagatggaa
     4561 aggtagcagt tagagggtgg gaaattgtaa gcgatgtaga ctcattaaca ttgtgtaaac
     4621 aacttgaaaa gataggtgta caaactattg tttatacaga tatatcaaaa gatggtatgt
     4681 tgcaaggacc aaattttaat atatatgaaa aaatagcaaa ggaaacatca ttaaatgtta
     4741 ttgcttctgg aggcgtaact tctatagaag acgtaaaaag actaaaagct atgaatttat
     4801 atggtgcaat aataggtaaa gcgctttatg ataaaaagat agactttaag gaggcacaac
     4861 aattatgctt actaggagaa taataccttg tctagatgtg agaaatggaa gagttgtaaa
     4921 aggaaaaaaa tttaaagata tagtagatgt tgatagtcca gaggtgcttg gaaaatttta
     4981 tagtgattgt ggtgcagatg aactcgtatt ctatgatata actgcatcaa atgaagagag
     5041 aactacgtct ttagaatttg tgacaaaagt tgcagaaaat ataagtatac cattttgtgt
     5101 tggtggagga gtaaataaat tagaagattt cactgatatt ttaagaaaag gtgctgataa
     5161 agtttctata aattcatcag cagtcaaaaa tcctgaatta ataagggaag cttcattaaa
     5221 atttggagca caatgtgttg ttttatcgat agatgcaaag aaaaatgaag agggttcatg
     5281 gagcgtatat gtaaaaggtg gaagagaaaa aactaatctg gatgcaattg aatgggctgt
     5341 taaaggtgta gaacttggag caggagaaat cgttgtaaat agtatggacg aagatggaat
     5401 gaaaaatgga tatgatattg aattattatc aaagataact tctttagtaa atgtacctgt
     5461 tatagcatct ggaggtgctg gaaaaatgga agatttctat caggcggtta ataaatctaa
     5521 tgtagatgga attttagcag catctgtttt tcattttggt gagataaaaa taaatgattt
     5581 gaagaaatat ttaaaaaaca tgggtgtaga agtaagatta taagttaaat attgaaagga
     5641 gattagaaat atgaataata aatgtaacga tatatacact gatgaagtag atgaatttat
     5701 aagaagtata aagtttgatg ataaaggatt agttcctgtt gttgttcaag aggtagtgag
     5761 taaagatgta ctgatgttag catatatgaa taaagaggct ataaagaaaa cattaaaaga
     5821 taaagtggct tgctatttta gtagaagtag acaagaactt tgggttaaag gtgagacatc
     5881 tggtaatact caaaaagtag ttaagatgtc ttatgattgt gatgtagata cgatactact
     5941 ttttgttgaa caaactggtg tagcttgtca tacaggaaat tattcttgtt tttacagaga
     6001 cttgtttgat gatactgcta aaatggaact tgaagtacaa acaaatatat tgaaagaatt
     6061 atatgattta attaatgaaa gaaaaaataa tccagttgaa ggttcttata ctaattatct
     6121 ttttgaaaaa ggaatagata agattcttaa aaaagtaggt gaagagagta gtgaagtaat
     6181 tatagcgtca aaaaataccg ataaaagtga acttatttac gagattagtg atttagtata
     6241 tcatacattg gttttaatga tagaaaaagg tgttgaaatt gatgaaataa aaaaagaatt
     6301 acttaaaaga agaaaatagg atagaataga ttaatgctaa taagaaaata taccgctttt
     6361 aataaaaagt ggtatatttt tttattttat attttcgatt tatagaataa tatttgaatt
     6421 acttgat
//
LOCUS       DAMJVM010000141         6076 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.146, whole genome shotgun sequence.
ACCESSION   DAMJVM010000141 DAMJVM010000000
VERSION     DAMJVM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.146"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            <1..734
                     /locus_tag="PC523_003297"
     CDS             <1..734
                     /locus_tag="PC523_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2476782.1"
                     /translation="RAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKL
                     SGGERRRLHLLKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYF
                     LDRICNKIFSYEGNGKIHIYTGNYSDYLIYREIQGIEFEESKKESVKKDDSITPKKEK
                     PKNDKKLKFSYNEQREFENIDSDIEKLESKIATLDESTSKFSTDFTKLQEILNEKSKL
                     EKELEYKYERWEYLNNLADEIANNK"
     gene            complement(983..1330)
                     /locus_tag="PC523_003298"
     CDS             complement(983..1330)
                     /locus_tag="PC523_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HDF2476783.1"
                     /translation="MSKSNDVLDIVFKITNGLEYEVDENSVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(1317..3248)
                     /locus_tag="PC523_003299"
     CDS             complement(1317..3248)
                     /locus_tag="PC523_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HDF2476784.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFLEGLAVGVGGVLFGIGVAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSTGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVS
                     GMTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGS
                     KNEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGS
                     LPWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEK
                     EKEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVL
                     VVLTALPIILSKVKNNEQE"
     gene            complement(3659..5848)
                     /locus_tag="PC523_003300"
     CDS             complement(3659..5848)
                     /locus_tag="PC523_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HDF2476785.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDKDILIEDL
                     EFVCKEEEKVLLEDFNDNQYEVPHDKSYVDLFREQVKVTPSNIAARDENRNLTYKELD
                     EVTDKLAGYLNYIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLEYELTEKITPSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSINATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYKKRNYELYNEYGPTEFTVISTSFHVDREYGKIPIGKPISNTYAYVLDKKNKLCPI
                     GVPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDEGDMYICAYYVIGDED
                     SDKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKKALPE"
BASE COUNT         2115 a         1048 c          677 g         2236 t
ORIGIN      
        1 tgagggctat agactatgta aaagaagcta gtgactatat agaaacttca gatggtacta
       61 ggattacagc ttctcaaatg tgtgaaagat tcctttttaa tggtactatg cagtatactt
      121 tgataggtaa attatctggt ggtgaaagaa gaagactaca ccttcttaaa atccttatga
      181 cagctcctaa tgtgcttctt ttagatgagc ctacaaatga tttagacatt gaaactctta
      241 aaatattaga agaatactta gacgaattta aaggtgtagt agttacagta tcacatgata
      301 gatatttctt agatagaatt tgtaataaaa tattttcata tgaaggtaat ggaaaaattc
      361 atatctatac aggtaattac agtgattatc ttatttatag agagattcaa ggaattgaat
      421 ttgaagaatc taaaaaagaa tctgttaaaa aagatgactc tattacccca aaaaaagaaa
      481 aacctaaaaa tgataaaaag cttaaattct catataatga acaaagggaa tttgaaaata
      541 ttgactcaga tatagaaaaa cttgaatcta aaattgcaac acttgatgaa agtacttcca
      601 aattttcaac tgactttact aagcttcaag aaattttaaa tgagaagtct aagcttgaaa
      661 aagaacttga atataaatat gaaagatggg aatatttaaa taatctagct gatgaaatag
      721 ctaataataa atgatattaa aataatctta agtttaataa gttttgcttt aaatctgtac
      781 tttataaata ttttaaagag tgtgtctcaa aatgaacttt ttagtttacg agacactctt
      841 tttatatgaa atcaaatata ttttcatcat ttcaacaata taaaagaggc ttatctctat
      901 tttgagacag cctctttcat aatttttata attaccttct aatttatatg ctctaatata
      961 tctataataa ttcttattat atctatatat ccagtctttc aatatagtga caattaactt
     1021 caatatgatt taaaaatact aacaaccttt cataaagtgg atgagattct tctccatatt
     1081 tagcttctag gttcttgcct atttctctaa cagttctttg accatcaatt tgtaaaaaaa
     1141 caaaacttga atattcatct agttctattt ttttgtattt aggtattcta gcttttaatt
     1201 tcctaaaaaa cttttgaatt ttatggtcct gtttttcaag tatagtaaca acactatttt
     1261 catctacttc atattcaagt ccattagtaa ttttaaatac aatatctaaa acatcattac
     1321 tcttgctcat tattttttac tttagataaa ataataggta atgctgtaag cacaactaaa
     1381 actactaata aagcaattgc cattgaattt gttgcagcaa atccacttgg tacctttgga
     1441 gttacaactc cagttacttg tagtattata cctattaaac caatgataga accaccagca
     1501 acaagaccag aagataaact tataccatta gaaactttaa cttctttttc tttttcagtt
     1561 ttagatacta attcaacaaa taaacgaatc aatgctccaa ctaatattat agaagttgtt
     1621 gcaattggta aatagaatcc tatagctata gtcattattg gtaaatttaa tgcatataga
     1681 acgattgcca taaatactcc aacgataatc ataacccaag gtaaactacc tgacatgata
     1741 ccagaagtta atgttgacat taagtttgct tgtggtaatg caaattgtac attatcacca
     1801 gttgctctaa gttggtcaga aagtaataaa attactccta caacaactat aacaccaact
     1861 atacttgcaa ttgtaaagta acgttgcatt tcgtttttgc taccaccaat aatataagtt
     1921 actttttgag attgagtata acctccagct atcgcaatag caacaactat aaatgaacca
     1981 aataaaagta atgatttatt agcttctaaa tcagtccagc ccattataac aaacactaat
     2041 gttacaataa ctagagaagc tatagtcata ccagaaactg gaagatttga tgttccgatt
     2101 gttcctgtta aacgaccagc tactatgaca aataataaag ataataaaag agatatgata
     2161 gctccaataa ttgccataac aatgttacca ccagaaatta agaatgctgc taaaaatccg
     2221 ataactactc cacctaataa aagtatcatt tgtatagatg aaccttcttc tccttctcca
     2281 gtacttgact tagctttaag tgtttctttt atagaagcga ttatagttgg tataagtttt
     2341 attgcaccaa taatacctcc acaaagcatc ataccagcac ctatatattt tacataacta
     2401 ctagatattg ctccaacatc catttggtta agaggcattg caggattatt ccataccata
     2461 gctccatctt tagccatatc tgtaaagtat cctattaaag gtgctatacc aaagtttgat
     2521 aaaatacttc ctgcaaacat tgttaaagaa acttcaagcc ctactataaa tccaattcct
     2581 aaaagtagag gatttacttc aatatcaaat ttccatttgt agaatttgct tcctacaaga
     2641 ctcattacat tgtttgctac atttaagaat gaaccagtta atacagtaat aaatccactt
     2701 attacaaatc caattcccat gtatttaatt gaatctccac cagcctctga agcaacaagt
     2761 gtctcagata tggccattga ttctgggtac ataagtttac catgttcttc aactattaga
     2821 tagttgtgaa ctatagcagc aactcctatc ccaaataaaa ctccaccaac tccaacagct
     2881 aaaccttcta ggaatgtaat ttggcttcca attaatataa ctgctggtag tacgaaaata
     2941 aatccacttg caacagattc tccaccactt gacatacctt ggattaagtt ctttccaaga
     3001 ataccctttg aacgagcaaa tgctccaaca aaagcagaac ctattatagc tcctggaata
     3061 cctgcggcaa ctgtaagacc agctttcatt ccagagtaag ttgtagatgc ggcaaaaatg
     3121 gcagctaaaa taataccgat aatcagtaca gctacatttc caccggttct ggacttatct
     3181 gtgatgtatg gaacgtaatc ttttccacta acctcaccat aagcaccctt aggtaatttt
     3241 ttgttcataa aattttcacc ccttttcaat ttgtatatat tataacatat atacatataa
     3301 aacttaactt tttattattt tacaaatagc gagccaaaac tactaaaatt taatactata
     3361 ttattaattt ctacttttag atatttttat gctaattttc tttttatttt tcaattctac
     3421 taaaaactat taagatataa aacaaaaaat ttttttaatg taaaattaat tttgtaaaaa
     3481 aagtgcttta aaacacaaaa aagtataaaa aatacaataa tcattcttat aaataaattt
     3541 tcaccatagt aaatatatac agaaaattct tttttttaca tcaattgatt ttcttataaa
     3601 aataggtgtc ataaagaaat atatgaaatc actttacaac acctgtcata ataaaaaatc
     3661 actctggtag tgctttttta tttacttttc ctattggtgt aactggtatt ttatctatcc
     3721 tcattattat tgttggaatc atatatggag gtagatgctc gtttaaatat tctctaatag
     3781 ttctttcatt tatcttatca ctatcttcat caccaattac ataatatgca catatataca
     3841 tatccccctc atcatttgtt ctcgatatac agacaacact tttaatttct gtaatattat
     3901 ttattatatt ttcaatctca tatagctcta tcctaaactc atcaattttt acttgattgt
     3961 ccatccttcc aatataatct aaatttccat catctaacca ccttactaaa tcaccagttc
     4021 tatacattaa cttattatgt tcacaagttt tgtaaggatt ctctacaaat acttgctctg
     4081 ttagtccttt tttatttaaa tatcctcttg atatttgaat gcctgatagg cataactctc
     4141 ctggaactcc aattggacat agtttgttct ttttatctaa tacatatgca taagtattag
     4201 atataggttt ccctattggt atttttccat attctctgtc aacatgaaaa gaagtcgata
     4261 taacagtaaa ttctgttggt ccatactcat tatacagctc ataattacgc tttttatatc
     4321 cttttaatct ttctcctcca gtctgtacaa ctctaagtga gttattattg caattttgta
     4381 taaataacct tgcaaacgct gttggcaacg ctagtatagt tatatcattt tcctcacaat
     4441 attcatttac tgtatattca tcaaatttag cttcttcagg cataatatct attgatgcac
     4501 ctacaagcaa ataagggaat agttgtttca ttacagcatc aaaactgaaa cttaagtata
     4561 taccacatat atcattttct gtagcgttta tagattttac actatagtaa cacatattta
     4621 ctaaactctt atgctctata gctatggtct ttggcctacc agttgaacct gaggtagata
     4681 tcgtatatgc acaatttgat ggtgttattt tttcagttaa ttcatattca agcttaaaca
     4741 tttcttcatc atttatatcg agtactatgg tattctcatt tacaacctta ctttttagtt
     4801 cgtctgtagt tatcactact tttgcattac tatcttcaag aaggtaattc aatctttctt
     4861 ctggattgct tgtgtctatt ggaaaaaatg ctccacctga cttcataatc cctatagctg
     4921 taataattat gttaatatct cttggtaaca ttacagctac tatgtcttca ctttccactc
     4981 ctatataatt taaataacct gcaagtttat cagttacctc atccaattct ttatatgtta
     5041 aatttctatt ttcatcccta gcagctatat tactaggtgt taccttaact tgctccctaa
     5101 ataaatcaac ataactttta tcatgtggta cttcatattg attatcatta aagtcttcca
     5161 ataatacttt ttcttcttcc ttacatacaa attctaaatc ttctattaaa atatctttgt
     5221 cttctattat ttgctctatt atataatcaa attgatttaa aattcttcta atagtctctc
     5281 ttttatatat agatggttta tactttattt ttattttaag cgctctctta gtatatacta
     5341 ttacagataa atcatatccc tttttttcta tgtcataatc atatgataaa tcaaaacttt
     5401 tagctataaa tattgtgttt ataagcttag tatttagatg atttttattc tctatatctg
     5461 ataataaata gtatccatac tctaaagacc tgctccactg cctttttagc tttcttacaa
     5521 catctataaa tttttcactt tgtctaactc gtactcttat tggaattata ttcattgttt
     5581 taccaaaaac tacatcatct tcatatatat acttttgaag tataagacca tatatagttt
     5641 ctatgataga gtctatgcta atattgttca ctttagaata ttgaattaac ctttcatgtt
     5701 tctcaatatt catcttaaaa acctcatcta ctacttcatc agaacaatct ttataaaaaa
     5761 aatcatttgg aatttttgct atattcgtat aatcatctaa caaatccatc caataattca
     5821 tagctcgttc taaattaact tcatacatat caaaattccc ccttactact aagtctatat
     5881 aaaaaattct tttggaattt tatttttcac aatatataat atattaataa tacatatgca
     5941 agctctaaaa gcatgagctt gcataaaatt acacctatta ttatggctac taaattcgta
     6001 atatttacaa tgtttaaaat atattataac acatatatga ttttattttt ctacatttat
     6061 ataatatata aattac
//
LOCUS       DAMJVM010000142         6031 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.147, whole genome shotgun sequence.
ACCESSION   DAMJVM010000142 DAMJVM010000000
VERSION     DAMJVM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6031
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.147"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            90..1388
                     /locus_tag="PC523_003301"
     CDS             90..1388
                     /locus_tag="PC523_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HDF2476786.1"
                     /translation="MNLTQKRWIILIASCFINLCIGSIYAWSVFASPMAEHLNSIAGT
                     TFTAASLAIVFTVTNSVGPITMITGGMINDKFGPKKVVFVGGLVFGIGMILAGFSKGL
                     PMLILSYGIITGLGVGMVYGCTISNSIKFFPDKSGLVGGITTAAYGFSSVIMPPVANW
                     IINKYGVTSAFKSIGLVFLIIVCVSSFFIKKCPADFTPEGWIPPEKKGKVRVIINKNW
                     KEMLSTPIFYLMIIILTCGAFYGLMCVSLASSLATNMIGMTSSDAAIVVSILALFNTL
                     GRIVAGYISDKIGRINTLAGALVISIFGLLCLLFSREGDVATFYIGIIIIGICFGSFM
                     GIFPGFTVDKFGPKNNSVNYGIMFIGFAFAGYVGPAVMKNVYATDGNYQRAFIIAIVI
                     ALIGFILTFLYKYINKIQNLKIENLMKGDIKDEKKISKSM"
     gene            1360..3309
                     /locus_tag="PC523_003302"
     CDS             1360..3309
                     /locus_tag="PC523_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2476787.1"
                     /translation="MKRKYPNLCKPIKIGNVTFKNRMFSAPMGGTDITADCTIGSKST
                     AFYELRAKGGAGAVTISECMVHPETDGSHAYHLDLKIVDSLASFTYTADAIRRHGAIP
                     SVELSHSGQYSGTYLVDKDKKRGLSQWGVSPSVRPDGLEIKELTEEIIAEIVESYGNV
                     AALAKRAGFEMIMVHGGHGWLINQFLSPYFNKRNDKYGGSLENRVRFAKEVLDSVRKA
                     VGPGFPIEFRMSGSELFEGGYDLDYGVEIAKLLESRVDLLHVSAGTYQRGFAVTHPSM
                     FLEHGCNVYLAEEIKKHVNIPVATIGALNDPEMMEEIIASGKADVIYMARALLADHEL
                     PRKVMSNQDEKIVKCLRCFTCMAERATTSTRRCTVNPLIGRELDGTEVIPVLKPKKVL
                     IAGGGPGGLQTAITAAKRGHKVILCEKTNELGGILKGEQALPFKYEMYELGNTFGKIA
                     EDLGVEVRLNTRVTKEYVENENVDALIIAVGSEPLVPSIEGLDGENVVIVNDYYLEKE
                     KVTDEVVVLGGGLAGCEAAIHLAQEGKTVHLVEMRAELAPDANIRNRPILLQEIEKQG
                     IYVYTEHKGLSVTTEGVVCMNKSGNKINIPGTSVICALGQKPRREVVDALLDCAPYVA
                     QIGDCVRASTITNAVYQGHHVALDI"
     gene            3747..4172
                     /locus_tag="PC523_003303"
     CDS             3747..4172
                     /locus_tag="PC523_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2476788.1"
                     /translation="MINENLKSLRKIHQYTQEELAEKLNVSRQSIAKWESGESIPDIG
                     SCIKLAKLYNVKLDDLVNHSEEKTGIIVPPKGKFFFGAVVVGERGQIVIPKEAREVFN
                     INAGDKLLVLGDEERGLGIVHQRDLINFIGHVGVVPKEE"
     gene            4204..5391
                     /locus_tag="PC523_003304"
     CDS             4204..5391
                     /locus_tag="PC523_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDF2476789.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVHRGNEVLYYSFNEFRDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPRLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQSFIEIIRIIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIESLGSIPNNF
                     KVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVANRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            5583..5987
                     /locus_tag="PC523_003305"
     CDS             5583..5987
                     /locus_tag="PC523_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HDF2476790.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNYVKPWKDAEIYIDKYMVK
                     QDISKDNVKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLKSNEELKSVKYPPIYLKYKPDK"
BASE COUNT         2290 a          647 c         1132 g         1962 t
ORIGIN      
        1 tatttacatg tttatattta catatagaaa aaattgtgat aatcttaact aagaaattcc
       61 atttaataaa tttatagagg aggagaataa tgaatttaac acaaaaaaga tggattatat
      121 taattgcaag ttgtttcatt aatttatgta ttggctcaat atatgcatgg agtgtatttg
      181 catcacctat ggcagaacat ttaaatagta ttgctggaac gacttttaca gctgctagtt
      241 tagcaattgt atttacagtt acaaactcag ttggacctat tactatgatt acagggggaa
      301 tgataaatga taaatttggt cctaaaaagg ttgtatttgt aggaggatta gtttttggta
      361 taggtatgat attggcaggt ttttcaaaag gattacctat gttgatttta tcatatggga
      421 taataacagg tcttggggtt ggcatggttt atggatgcac aataagtaat tcaattaaat
      481 tttttccaga taaaagtgga ttagttggtg gtataacaac agcagcttat gggtttagtt
      541 cagtaataat gcctccagtt gctaattgga taattaataa atatggagta acctctgcat
      601 ttaaatcaat aggtttagta tttcttataa ttgtatgtgt ttcttctttc tttattaaaa
      661 aatgtccagc agatttcaca ccagaaggat ggataccacc agaaaaaaaa ggaaaagtaa
      721 gagttattat taataagaat tggaaagaaa tgctttcaac acctatattt tatctaatga
      781 ttataatatt aacttgtgga gctttctatg gattaatgtg tgtgtcatta gcatcttctc
      841 tagcaacaaa tatgattggt atgacaagtt cagatgcagc aatagtagtt tcaattttag
      901 ccctatttaa tacattaggc cgtattgttg cagggtatat ctcagataaa attggtagaa
      961 taaatacttt agcaggtgca ttagttattt caatttttgg tttattatgt ttattattct
     1021 caagagaagg agatgtagca acgttctata ttggaataat aattataggt atatgctttg
     1081 gttcatttat gggaattttc ccaggattta cagtagataa atttggacct aaaaataata
     1141 gtgtaaatta tggaatcatg tttattggat ttgcttttgc aggatatgtt ggacctgcag
     1201 ttatgaaaaa tgtatatgct acagatggca attatcaaag ggctttcatt atagctatag
     1261 ttatagcttt aataggtttt atattaacat ttttatataa atatataaat aaaatacaaa
     1321 atttaaaaat agaaaattta atgaagggag atataaaaga tgaaaagaaa atatccaaat
     1381 ctatgtaagc caattaaaat tggaaatgta acattcaaaa atagaatgtt ttcagcacct
     1441 atgggaggaa cagacataac agcagattgt acaattggct caaaatcaac agccttttac
     1501 gaactaagag caaaaggtgg agcaggtgca gttacaatta gcgaatgtat ggttcatcca
     1561 gaaacagatg gttcacatgc atatcatcta gatttaaaaa tagttgactc tttagcaagt
     1621 tttacttaca cagcagatgc aattcgtcgt catggagcaa taccaagtgt agagttatct
     1681 cattcaggac aatattcagg aacttattta gttgacaaag ataagaagcg aggattatca
     1741 cagtggggag taagtcctag tgttcgtcct gatggattag agataaaaga gcttacagaa
     1801 gaaataattg ctgaaatagt agaatcgtat ggaaatgttg cagcattggc taaaagagca
     1861 gggtttgaga tgattatggt tcatggtgga catggatggt taatcaatca attcttatca
     1921 ccttacttta acaaaagaaa tgataaatat ggtggttctt tagaaaatag agttcgtttt
     1981 gctaaagaag ttttagatag tgttcgtaaa gcagtaggtc caggattccc aatagagttt
     2041 agaatgagtg gttctgaatt atttgaggga ggatacgatt tagattatgg agtagaaatt
     2101 gctaaattat tggaatcaag agtagattta cttcatgtat cagcaggtac atatcaaaga
     2161 ggatttgcag taacacatcc ttctatgttc ttagagcatg gatgtaatgt atatcttgca
     2221 gaagaaatta aaaaacatgt aaacattcca gttgctacta taggagcatt aaatgaccca
     2281 gaaatgatgg aagagataat tgctagtgga aaagcagatg ttatttatat ggctcgtgca
     2341 ttactagctg accacgaatt accaagaaaa gttatgagta atcaagatga aaaaatagta
     2401 aaatgtctaa gatgtttcac ttgtatggca gaacgtgcta ccacttctac aagacgttgt
     2461 actgttaatc cacttattgg acgtgaatta gatggaactg aagttattcc agtattaaaa
     2521 cctaaaaaag tattaatagc aggtggtgga ccaggagggt tacaaacagc tattacagct
     2581 gcaaagcgtg ggcataaagt aatactttgt gaaaagacta atgaattagg tggaatttta
     2641 aaaggagaac aagcacttcc atttaaatat gaaatgtacg aattaggaaa tacttttgga
     2701 aaaatagcag aagatttagg tgttgaagta cgtttaaata caagagttac aaaagaatat
     2761 gttgaaaatg aaaatgttga tgcattaatt atagcagtag ggtctgaacc tcttgttcca
     2821 tcaatagaag gtcttgatgg agaaaatgta gtaatagtta atgattatta cttagaaaaa
     2881 gaaaaagtaa ctgatgaagt agtagtttta ggtggaggat tagcaggatg tgaggctgca
     2941 attcacttgg ctcaagaagg gaagactgtt cacttagtag agatgagagc agaacttgcc
     3001 ccggatgcaa atataaggaa tcgtccaata ttactacaag aaatagaaaa acaaggtata
     3061 tatgtatata cagagcataa aggtttaagt gtaacaacag aaggtgtagt atgtatgaat
     3121 aaatctggga ataaaataaa tattccagga acttcagtaa tatgtgcact tggacaaaaa
     3181 ccaagaagag aagtggtaga cgctttactt gattgtgctc catatgttgc tcaaattgga
     3241 gattgtgttc gtgcatctac aataacaaat gcagtatacc aagggcatca tgttgcgcta
     3301 gatatatagt taaaaagaag tagatagtat tgttttatta taaaaaagga gtgtctcaaa
     3361 atgaactttt tagttcatga gacacttttt tgcgtgaaat caaatatatt tttatcattt
     3421 caataatgaa aaaagattat ctttattttg agacagcacc tttgttatta taattttaat
     3481 tcttattgca agttttcatt tgattgatga gtaaaacttt ttaaggtgta gaagtattta
     3541 acaatgtaaa tgactctaac ttaaatactt tagcaaaagt tgatagattt gtgcttgctt
     3601 ttatttatta taacttaagg aagtatgtta cctaaacaat aaatttatct aataaaaagt
     3661 atataaatat atatatgaat gttaagatta gttaatagaa tttaaataat aaatccaata
     3721 ttataaacat aggaggtatg aaaaatatga ttaatgagaa tttaaaaagt ctaagaaaaa
     3781 tccaccagta tacacaagaa gaattagctg aaaaacttaa tgtttctaga caatcaatag
     3841 caaaatggga aagtggagaa tctattcctg atataggtag ttgcattaaa cttgcaaaat
     3901 tgtacaatgt caaactagat gacttagtta atcattcgga agaaaaaaca ggtattatag
     3961 tacctcctaa aggaaagttt ttctttggag cagtagtagt tggtgaacgt ggtcaaattg
     4021 tcattccaaa agaagcaaga gaagtattta atataaatgc aggagataaa ttattggtac
     4081 ttggagatga ggaaagagga ttaggaattg ttcatcaacg tgatttgata aattttatag
     4141 gtcatgtggg agtagtacct aaagaagaat gattttaaaa aattataaaa ggaggtatga
     4201 aatatgagta aaatagtatt ttttagcatt cctgcatatg gtcatactaa tcctactatt
     4261 gaagtagttc gtgagttagt gcatagagga aatgaagtat tgtattattc ttttaatgaa
     4321 tttagagata aaattgaagg tgctggagca aaatttattt gttgtgataa ataccttcca
     4381 gaactacttc ctggagatga gaaaaaaatt ggtaaggatt ttcccagatt aattgagatg
     4441 atagtagata ctactatttc tttagatgaa aaagtttgta gagaacttaa agattttaat
     4501 ccagattgca ttgtatcaga ttcattaagt ttttggggaa agttatttgc acaaaaactt
     4561 aatattccat atgtatgctc tacaacaact tttgcattta ataaacatac agcaaagttg
     4621 atgaaacaaa gctttataga aattatacgt ataatttttg gtgtaagacg tattaataaa
     4681 aaaataaagc ttttacaaag aaaagggtat gaagtaaaaa attttatttc tatagtttct
     4741 aatgataatg atacggatac tattgtatat acatcaaaag aatttcaacc aatggtagaa
     4801 accttctcta gtaagtattc atttgtaggt ccatcagtat caaagttgat tattgaacct
     4861 aaagagagaa aaagaaagct aatatatatt tcgcttggta caattaataa taaaaatgtt
     4921 agcttttata aaaactgtat aaatgcattt aaagattctg atgttgatgt gattatgtct
     4981 gttggtagaa gtactgatat tgaaagtttg ggaagtatac caaataattt taaggttaaa
     5041 aatagtgttg aacagattgc tatacttcaa aaaactgatg tgtttgttac tcattctggg
     5101 atgaatagtg ttaatgaaag tttatattat ggagtaccca tggtattatt tcctcaacat
     5161 tcggagcaga gaatggtagc taatagagtt gttcatttgg gagcaggaat tatgttaaaa
     5221 gaggataagt cagaaagtat taaaaaagca gtatttcaag ttataaatga caatgaatat
     5281 aaagaaaatg caactaaact atcaaaaagc ttttataatg caggaggctc aaaaaaagca
     5341 gcagatgtaa tattacagat tatatgtaat agtagacaag ttgatatata atagattttt
     5401 taaaaatata tatacataca tgcaatttaa tcaaagtact gacagtgggg ccttttaacc
     5461 tactagttgg ggcatatata ttgatgctaa atatgaggga tgttatattt taataaaaaa
     5521 gataacatcc tttttttaat gcattatatt aagttttaaa caataaatga aggaggatta
     5581 atatgaaaaa atataaagtt atttttatag tattaattat aggtattata agtgtaacag
     5641 ggtttgttgt atataaaaac tatgttaaac catggaaaga tgctgaaata tatattgata
     5701 agtatatggt aaagcaagat atttcaaaag ataatgtaaa aagtattaca aaagaaaaag
     5761 ccaagaaggc aagttatgaa ggaatactat acaaagtgta ttacaaagat gaccctcgat
     5821 atgagtatca atatttttat agtgataatt attatgctct atatgtaaat aaagtaatgt
     5881 tacaaattta tgatatggaa aataataata aacttttaaa aagtaatgag gaattaaaaa
     5941 gtgtaaagta tccaccaata tatcttaaat ataagccaga taaatgattt atgacaaaat
     6001 agaaggattt ttattaaaat ttatttaaaa a
//
LOCUS       DAMJVM010000143         5971 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.148, whole genome shotgun sequence.
ACCESSION   DAMJVM010000143 DAMJVM010000000
VERSION     DAMJVM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.148"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            107..1474
                     /locus_tag="PC523_003306"
     CDS             107..1474
                     /locus_tag="PC523_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDF2476791.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILSYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIITFICREHECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            1499..2107
                     /locus_tag="PC523_003307"
     CDS             1499..2107
                     /locus_tag="PC523_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDF2476792.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHEKLFIEIAIEAFKNFDELIIISNNEELYSKYDIRVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNSEVVERIANKMKDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYKKLTKV"
     gene            2155..2526
                     /locus_tag="PC523_003308"
     CDS             2155..2526
                     /locus_tag="PC523_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDF2476793.1"
                     /translation="MNLLKCDSWAVILNNSNKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            2684..3022
                     /locus_tag="PC523_003309"
     CDS             2684..3022
                     /locus_tag="PC523_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDF2476794.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            3489..3992
                     /locus_tag="PC523_003310"
     CDS             3489..3992
                     /locus_tag="PC523_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HDF2476795.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNRIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEILRGQVTE
                     CATKKSK"
     gene            4039..4998
                     /gene="moaA"
                     /locus_tag="PC523_003311"
     CDS             4039..4998
                     /gene="moaA"
                     /locus_tag="PC523_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDF2476796.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKVRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRILELEKCGLNKVNISLDSLKEYKYKSITRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            5002..5472
                     /gene="moaC"
                     /locus_tag="PC523_003312"
     CDS             5002..5472
                     /gene="moaC"
                     /locus_tag="PC523_003312"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDF2476797.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDIDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            5504..5935
                     /locus_tag="PC523_003313"
     CDS             5504..5935
                     /locus_tag="PC523_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDF2476798.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENITTEGIEVFSLPIGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVQVLKEGIINVNDSIDVI"
BASE COUNT         2515 a          486 c         1104 g         1866 t
ORIGIN      
        1 ggagaatact tacttatgat aggaaaaaat gttaaaataa tagtgtaatc aaatataaag
       61 gactgaaata attaacagtt attaaatatt aggagtgtaa aaatttatgg gattaaaaga
      121 atttgatttt attgaagagt ctatcgatat gttgaggaca atgtcaccaa cattagaaac
      181 tatatcagat gaaatagaag agtattttga aaatatcttg gatgaaaaga atcaagaata
      241 tataaatgta acctctagaa taaaatcaga atcaagttta agagaaaaga taattagaaa
      301 tagatattta aaaaaatatg gagaagcaag taatcttatt cataatgtat ctgacttaat
      361 aggtttaaga atcgagtgca gatttattga ggatgaaaac aaaatatata gacttttgag
      421 aaggtatttt aataaaacag atgacaaaat aaactattat aacaaagaga ataagaatat
      481 taaattaaaa ctatctgaga ggcaacccaa taagcaaaaa aatggatttg aaatatataa
      541 aatagatgga gttttcgcgt atttagatag agaagtaaaa tttgaacttc aaattaaatc
      601 tcttgtaaat gtgttttgga gtgaaataga acataaaata atttataaaa acaatacata
      661 tttacttgct gacaaattta taaaggatat gatggattct ataaagaata atcttactat
      721 gatagataat cagcttttaa gtatttataa aaactttcat tcagggaagt cttttaatat
      781 gaaagtgagt aaaaaagaaa ttgaaaaatt atttgcgaaa ctagtatatg acgctttttc
      841 tgaaaagatg aataaaagta taggttttgt tgtagatttt aaaaaaccat gtgagactat
      901 attgagctat tcttttaata agcaagatat aagtgatgaa gttttaggta attttatgct
      961 tgatgagttt gcaagattaa atgaaattgt aaacaaggat atagatttta atgagcaaat
     1021 agaatttgaa agagaaccga catttgatga taagttttgt aaagacctag gaaatcattt
     1081 tagaagtaga ttaaacacag aatttccatg gaacctgttt tttagaatat tatttgaaat
     1141 agagccatat aacaatacca aagactttga aaattttgta gagtttataa aagaaaatgt
     1201 gctactagaa gaaaataggg aacaattatt atgccaattt gaagaagata gcaaacttat
     1261 aattgaagat atatatgatt gcatatttaa tagtataagt gagattgata gtgttgaaat
     1321 tttatataat tataatcttg aaaaaataaa ctttacatca agtgaaatta taacttttat
     1381 atgtagagaa catgagtgtt atggtgaata cttagaagaa aaagaaaagc ttatgaatac
     1441 atttaaagaa aagattattc aaatctttga ataataagtc aatgctgaag tggcgctaat
     1501 gcgccatttt tcaaatatat atgaattaag taaaattatt ttggaggagg cgatttgtat
     1561 agagaaaaca ggtgtaattt tggcaggggg aaaaaattct agaatgggta gagataaagc
     1621 ctttctagag ttacatgaaa agctttttat tgaaattgca atagaagcat ttaaaaactt
     1681 tgatgagttg ataataatat ctaataatga agaattatat tctaaatacg atattagagt
     1741 ttataatgat atagtaaaag atgttggtcc aattggagga atatatactg ctcttgaata
     1801 tgcaaagtat gatatagtta caattgcatg tgatatgcca tatctaaata gtgaagtagt
     1861 cgaaagaata gctaataaaa tgaaggataa aagtgtgatt tcagtgacaa atggaaagtt
     1921 acaaccattg tgttctggat ataaaaagag tataataaat aaagtatctt tatgtataag
     1981 ggaaaatgat ttaaagctca gaagctttat agataaaata gataaaagct atatatactt
     2041 tgatgaagaa gatttatttt taaatgtaaa cactgttgat gagtacaaaa aattaactaa
     2101 agtttaatta gctatggttg taaaatagag taggatttat atggaggaga gtttatgaat
     2161 ttacttaaat gcgattcttg ggcagtaata ttaaataata gcaataaaga aagtaaagca
     2221 tataaaatat tagatgaact taaaaggaat atgtacaaag ttgttgctat agatgaagag
     2281 aagaaaccta ttgaaggtat agatgtgtat gaatgtttaa aagacgttcc acacaatatt
     2341 gatgttgtag ctattattga taaacaatct aagatggatg taattttaga agaagtagag
     2401 cttttggata tacaaaatat gtggtttgaa aaaggaagct ttagtgaaat gatgattaaa
     2461 aagactaaag atttaaaatt aaacatagaa tataatttaa gtttatatga tgagttgact
     2521 agataaaatt atgtacttta ataataaaaa tataattagt tagcaaaaca tattatttca
     2581 tagtatagat tggttaatga aacattaaat tacaatattt gttaaaaaat tatgagttac
     2641 atgatataat atattaaata ggatactaag gagttggtat attatgttaa tagtaactac
     2701 agaaaaagta gaaggcaaaa agatatcaaa agttttaggc ttagtgagag gaagtacaat
     2761 aagagcaaaa catgttggaa aggatatagg agcaagtttt aaaaatcttg ttggaggaga
     2821 acttactgga tataatgaaa tgcttactga agcaagacaa attgccatag gtagaatggt
     2881 tgaagatgca gaagctaaag gtgcaaacgc agtaatagca tttagattgt cttcagcatc
     2941 agttatgcaa ggggcagcag aaatgcttgc ttatggaaca gcagttgttt tagaagatga
     3001 taatagtatt cttgaaaaat aaaatataaa tagttaaaaa gaggcatctt ttaaaaattt
     3061 tatggatgcc tttttttatt tgaaaaaact aatttcagaa gaaattttaa ttattatgac
     3121 aataaaatta gggtgatatt atataaataa gtaaaattat attatataat gtatggtata
     3181 gactttaaaa attttattgg tagagaaaaa tatggacaat gttataataa ttatagaaat
     3241 taaaagtact tacattcatt atatatgtta catgtataat tagttttata tttaatgcag
     3301 ttttgaagta ctatcttcat tttgagaacg ttggaataat aaaaaatgta tgtattataa
     3361 gtgatataaa taaaaaataa atataatatg ttgtattgct gatagaaaag ggaataaagt
     3421 aaagtaggtt ttattgaaag agatttttat ttataggact taatcaatgt atagaggggg
     3481 gagagcatat gtttaatgtt gcaattataa cacttagtga taaaggatat gaaggaaaaa
     3541 gagaagatat aacaggaaaa aaattaactg aatttgttga aaatacagga gcttataaag
     3601 ttactgaata tgtacttata aaagatgata aggaaatgtt aaaggaaaat attataagac
     3661 tttgtaatag taatagaata gatttaattc ttacaaatgg aggtactggt ttttcaaaaa
     3721 gagatataac tccagaagca actaaggaag tattggaaaa agaaatacca ggtctgagcg
     3781 aatatatgag aatgaaatct acagaaatta ctaaaaaggc tatactaagt aggggtgtga
     3841 gtggaataag aaataattct ataataataa atttacctgg aagtcctaaa ggtgcagttg
     3901 aaaatttaag ttttataatt gatgttttag atcatggcat agaaatttta agaggacaag
     3961 ttacagaatg tgccactaaa aaaagtaaat agtatataat ctagatagat aaaaaataga
     4021 caaagaggta gatagagtat gattgataag tatggaagaa aaatagacta ccttcgtatt
     4081 tcacttacag ataagtgtaa tttaaggtgt gtctattgta tgccaccaga tgtaaaattt
     4141 gataaaaatt atataaatga aaatctaagt tttgaagatt acaagttcat tataaaggct
     4201 atggctgaac aaggtataac aaaagttaga tttactggag gagaacctct tttatatcct
     4261 aaattaaatg agcttataaa atttacaaaa gaagaatgtg gaataaataa tataggcatg
     4321 accactaatg ctattgggct ttctgataga atacttgagc ttgaaaaatg tggcttaaat
     4381 aaagttaata taagtttaga ctctttgaag gaatataagt ataaatccat aacaagagga
     4441 ggaaacttaa aagatgtttt aaaatcaatt gaatcttgtt taaatttagg tataaaggta
     4501 aagttaaatt gcgttgctat aagtgggttt aatgatgatg agcttaaaga ttttatgttt
     4561 ttaacagtta aatcaccaat tgatgtaaga tttatagaac ttatgccatt aggtgaggct
     4621 aatagagttt ataagaaggg gtattttaat gtaaaagaaa tgattgaaaa aatatatggt
     4681 ttatataaag tcaaaaatga tgaaaagagt acagcagaat attatatgtt taaaggagct
     4741 aaaggaagga taggaattat aactccatta agctgttcat tttgtaacac ttgtaatcgt
     4801 attagactta cttctagtgg tgcaataaaa ttatgtctac attcaaaaga agaaatagat
     4861 ataaaaccat ttttacataa accattgtta tttagagaat ctatgaaaga tataataaaa
     4921 caaaaaccag aaaaacatca tttaatagaa aataaatgta gtgatacaaa taggcgtatg
     4981 tatgaaattg gaggataagt aatgttaaca catataaatg aaaaagggta tgccaagatg
     5041 gtggacataa gtgaaaaaga tgatactaaa agaacagctg tggctacagc tacaattact
     5101 cttagtaagg aagctttaga aaaagtaaaa aatggtcaga taaaaaaagg agatgtattg
     5161 tctgtggctc aagttgcagg tataatggga gctaaaaata catcttcaaa cataccaatg
     5221 tgccatcaga ttaaattagt tggatgtgat ttagaatttg atatagatga ttctatgtct
     5281 gctattaaga tatatgcaac ctgtaaatct cttgggaaaa ctggagtaga aatggaagct
     5341 ttaaatgcat gttctttagc agcacttaca atttatgata tgtgtaaagc agttgataaa
     5401 aaaatggtta taagtaatat ccatcttgtg gagaaaactg gtggaaaatc aggagatttt
     5461 aagttcgagt aaattaataa tatattgaaa atttggagga aatatggcta aggtagtgtc
     5521 aataaatata agtgagaaaa agggtgtaat taaagttcct gtacaatgtg cagagctaaa
     5581 aataaatcat gggatagttg gagatgcaca tgcaggaaac tggcatcgtc agataagctt
     5641 acttggaaaa gaaagcatag ataaaatgaa aatgaaaggc tttgaccaat taaaatatgg
     5701 agattttgct gaaaatataa ctacagaagg tatagaagtt ttttccctgc ctattggtac
     5761 aaagctaaga atagggcaat gtgaagttga agtaactcaa ataggaaaaa aatgtcacaa
     5821 tggatgcgaa attaagaaat taactgggga ttgtgtaatg ccacgagaag gaatttttgt
     5881 acaggttctg aaagaaggaa ttataaatgt gaatgattca atagatgtaa tttaatgaag
     5941 ttagtattaa aatataatta taagtaaata t
//
LOCUS       DAMJVM010000144         5944 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.149, whole genome shotgun sequence.
ACCESSION   DAMJVM010000144 DAMJVM010000000
VERSION     DAMJVM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5944
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.149"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            160..900
                     /locus_tag="PC523_003314"
     CDS             160..900
                     /locus_tag="PC523_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDF2476799.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKDLN
                     IKYIFDYRSDQEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIDYKPKFKGIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YTSVEAYLYGEFNLNKEEIKKLRNQYLE"
     gene            912..2690
                     /locus_tag="PC523_003315"
     CDS             912..2690
                     /locus_tag="PC523_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2476800.1"
                     /translation="MSLNITARQVNIIKILLNSKDTISGLALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFEEINLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRILEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIREKKLLIKKLLLDILNENGLVLTDIEFKNVLNNILVMLS
                     RHKYNEKEFIENYVENYRRKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSDKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKVFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYELIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            2857..3774
                     /gene="pfkB"
                     /locus_tag="PC523_003316"
     CDS             2857..3774
                     /gene="pfkB"
                     /locus_tag="PC523_003316"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDF2476801.1"
                     /translation="MIYTVTLNPSIDYIVKLNELKTGSTNRVNEEYVYPGGKGINVSR
                     ILKELGNDNISLGFISGFTGEYIIRTLEEKNLKTDFIKIKNGFSRINVKIKESEETEI
                     NGQGPNIDDEDIDILYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            3811..5730
                     /locus_tag="PC523_003317"
     CDS             3811..5730
                     /locus_tag="PC523_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2476802.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAIFAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSNSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIATKDNNTNKYPQVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YAINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT         2443 a          555 c         1077 g         1869 t
ORIGIN      
        1 gtaaaattta ggttaatttt gtataaagat atacttttat attttacaat tgctaaccaa
       61 atactagaaa atattttaaa taaagaactt aaaattgtga taaaattaat ttattgatat
      121 ttaaaaaagt atataatatg gtttttaaga ggtgttaaga tgaaaaattt tagagattta
      181 ggtggtaaca aaactgagga tggtaggact gtaaagaagg gattgtttta taggtctgca
      241 aaattatcaa atttaagtga aaatgatata aagattttaa aagatttaaa tattaaatat
      301 atatttgatt atagaagtga ccaagaagct cgtaaacatc catcaacaat aatatcaaat
      361 ataaaaaata taagaatacc agcaatgaga gagttagaag aatcaggagg aagttttggt
      421 tctattgaag atatgattga tggattgttt gaaaaagatg gtgcatttaa tatgttaaat
      481 aatagctact ataatttgcc tataaataat ccttcatata aaaaactagt agaacttata
      541 agagactatt ctaatttacc aatattaaat cattgtacag ctggtaagga tagaactggt
      601 gtagggagcg ctataattct tatgatttta ggggtatcaa gagagaatat aatgaaagat
      661 tatctaaaaa gtaatgattt tgcagataaa gagatagaaa gatttataga ttataaacct
      721 aaatttaagg ggattccaaa agaaaattta aaatatatat ttggtgtaaa tgaagagtat
      781 atgaaaactg catttagaag aatagatgaa gaatacacta gtgtagaagc atatttatat
      841 ggagaattta atttaaataa agaagaaata aaaaaattaa gaaatcaata tttagagtaa
      901 aaggtgattt tatgagttta aatataacag caagacaagt aaatataata aaaatattgt
      961 taaattcaaa agatacaatt agtggtcttg ccttaagtca agagatagga tgttcttcga
     1021 aaactataca aaatgaaata aaggatatta ataaacaact tagaaatggt aaaatcttgt
     1081 caataagagg agttggttat aaactagaag gaagttttga ggagataaat ctaaatagta
     1141 atctctatga tgatgtagat agagtagaat atataattag aaaaatatta accttaagta
     1201 atgaagaaaa aaatactata aaacttgaag atttagccga ttctatgtat gtaagtgttt
     1261 cgacagtaaa aaatgattta aaagaagtaa aaagaatatt ggaaaagtat tcaatatcag
     1321 ttaaatctaa gcataaacaa ggtatatgtg ttttggagag tgaggataag atattaaact
     1381 gtatagttga cttaagcaat aagagagata atcaattgag tttaaatgat tttttaaatg
     1441 atgacataag ggaaaaaaaa ttactaataa aaaaattact attagacata ttaaatgaaa
     1501 atggattggt attaactgat atagaattta aaaacgtgtt aaataatata ttagttatgt
     1561 taagtagaca taagtataat gaaaaagaat ttatagagaa ttatgtagaa aattataggc
     1621 gcaaaagaag ctcaatttta gaaaatgaac aaaacaaaga aattataata aattcaataa
     1681 aaacattttg taaaaattta aaattagcta catcaataga tataagtaat gataaggtat
     1741 ttgaagaatt tttatataag catattagta gtttatgtaa aaaaataaag ctaggtataa
     1801 atcaatctcc tataatggct caagatataa aaataaaata tccatttgct tttgaactag
     1861 ctaatatagc taaaaagaca atagaaaaag atttgaagat agaaattaat gaagatgaag
     1921 ttgcaaacat agctctacat ataggaggag cagttgaaag agcatcttat aataataaag
     1981 ataaggtttt caaaactata atagtctgta cttcaggtat aggtacatca atgttaataa
     2041 aagctaaatt agagaatata tttaaggaaa aattagaaat attaaagatt attccttcat
     2101 acttagttga atatataaaa gttatagatg ttgattttgt aatatctact gttcctattg
     2161 aagtaggaga tgtaccagta ataaatatat ctccgatttt aagtgataaa gaaattagat
     2221 taattgaaaa atatatagaa acaggaaata tatatctaga tttagatata aaaagcctgt
     2281 ttgatagtga attatttttt actgatttgg tgtttaaaag gaaagaagaa gttatagatt
     2341 ttatggcaag taaacttgta caaaagggat atatagatga ggatatgaaa aaatcttact
     2401 ttgagagaga aaaaatagca acaacagaaa tagggaatat ggtaaccata ccacatggtg
     2461 ctattggaaa agtttttgat aataaaatag caattggtat attaaagaaa cctatttctt
     2521 gggaaatatc agatgttcgt ttaataataa tgttagctct tgataaagac aaaatattag
     2581 attatgaatt aatcttttca aaaatatata aaagagtaga ttccatagca aaagttataa
     2641 gtatatgcga aaatagcagt tttgaaaaat ttgtaaattt atttaattag atgggtggta
     2701 ttgtatatac atctgtaaat ttggataata taaaactaaa taaaataaaa acgataaatt
     2761 tctcttttaa ttaagagaaa ttttttgact ttcaaggata ttaatataaa gttaagatat
     2821 aaacataagg taaataaata aaaaagaggt ggcaatatga tttatacagt gacattaaat
     2881 ccatcaatag attatatagt taagctaaat gaactaaaga caggaagtac aaatcgtgta
     2941 aatgaagaat acgtttatcc aggaggaaaa ggtataaatg tatctcgtat acttaaagaa
     3001 cttggaaatg ataatatatc tttaggtttt ataagtggtt ttactggaga atatataata
     3061 agaacattgg aagaaaaaaa tttgaagaca gattttataa agataaaaaa tggtttttca
     3121 agaataaatg taaaaataaa agagtcagag gaaactgaga ttaatggaca aggtccaaat
     3181 atagatgatg aagatattga tattttatac aaaaaacttg ataagctaaa tcaagatgat
     3241 attttaatat tggcaggaag tataccatct actttagatg aaaaactata cgaaaatata
     3301 atggctagat tagaaaaaaa gaatataaaa gttgtagtag atgcaactaa aaatcttctt
     3361 cttaatgttt taaaatataa accttttcta attaagccta ataacgacga attagaagaa
     3421 ctatttggtg ttaaattaaa ttcaattgaa gacatggtaa aatatgctag aaggttaaag
     3481 gaaatggggg ctataaatgt cttggtatca atgggtaaag atggagcatt gttgattaca
     3541 gaggaagatg aagtgcttat aagtgatgtt cctaaaggaa aagtaaaaaa ttctgtagga
     3601 gcaggtgatt caatggtagc aggatttata tctgggtatt tgaatactgg taaatatgat
     3661 tatgccctta aattgggagc agcttcaggt agcgcaactg ccttttcata tgacttagcc
     3721 aaaagagaat acattgataa attggtaaat gaaatatctg taaagcagtt ttaaaatatt
     3781 aaaaaatata tagaaaggta attaatattt atgaaaatta tagatttatt agatgaaaag
     3841 tcaattaagt taaatttaaa ttcaaaaacc aaatcagaag caatagaaga attagtagat
     3901 ttggtcgcaa atagtggaaa tttaaatgat aaagaaaact acaaaaaagc aatttttgct
     3961 cgtgaagaaa tgagtacaac aggaattgga gaaggtgtag ccatacctca tgctaaaaat
     4021 tcttcagtta ctaaagcttg tatagcagca gcagtgtcaa aagaaggtat agattatgaa
     4081 tcgtttgacg gaagtttatc aaatctattt tttatgatag ctgcaccaga cggagctaac
     4141 aatactcatc ttgaagtatt atcaagatta tctacaatac ttatggatga agactttaga
     4201 aataaactta taaactcaaa ctctgaaaaa gaatttaagg aaataataga taaaaaggaa
     4261 cgagaaaaat tcagtgaaga atatcaagat gagaaagttg ctgaaaaaaa tataatagct
     4321 actaaagata ataatactaa taagtatcct caggtattag cagtaacagc atgtccaact
     4381 ggaatagcgc atacatttat ggcagcagaa agtttaaata aaatggccga aaataaaggt
     4441 gtatcaataa aagtagaaac taatggttca gcaggtgtta aaaataagct tacaaaagat
     4501 gaaatagaga atgcaacatg tataatagta gcagcagata aaaatgttga gatggctaga
     4561 tttaatggca aaaaagtcat aaaaactaag gtggctgatg gcatacacaa agcggaagag
     4621 cttatagaca aggctgtaaa tggagatgct ccaatttacc atggaggaga tggaagtcac
     4681 aacgaatcaa atgaagaaag tgaaagtgga tttagaaaag tatacaaaca cttaatgaat
     4741 ggtgtatcta atatgttacc atttgttatt ggtggaggta tacttatagc gatagccttt
     4801 ttacttgatg attatgctat taacccttca aactttggtt caaatacacc gatagcagca
     4861 ttttttaaag gaataggaga taaagctttt ggatttatgt tacccgtact tgctggatat
     4921 atagcatact ctatatctga tagaccagcg tttgttgtag gatttgtagg aggtgctcta
     4981 gcaggtgatg gtggttcagg ctttttaggt gcactacttg caggttttat agcaggttat
     5041 ttagtagaag ggcttaagaa aatatttagt gtactaccag cttcattgga aggaataaaa
     5101 ccagtattgt tatacccttt acttggaaca cttttgatgg gaattataat gacattttta
     5161 gtaatacctc cagttacagc aattaataat gcaatggttg gatttttaaa tggtttaggt
     5221 ggaacttcaa aaatagtttt aggattagta ttaggtggaa tgatggctgt ggatatgggt
     5281 ggacctgtaa ataaagcagc atatgtattt ggagttgcat cacttgaatc agggcagttt
     5341 gaaataatgg cagcagtaat ggctggtggt atggttccgc cacttgctat agctcttgca
     5401 actacatttt ttaagaatag atttactaaa gaagaaagag attctggtaa agttaattat
     5461 gtaatgggat tatcatttgt tactgaagga gctatacctt ttgcagctgg agacccactt
     5521 catgttatac cagcttgtgt tggaggttca gcagtggcag gagcacttag tatgttgttc
     5581 aacgcagctc ttcgtgcacc tcatggagga gtatttgtta taccagtggt tacccatcca
     5641 tttgcatata tattagctat agctgtaggt gctttggttg gaatgatgct tttagcatta
     5701 cttaaaaaac cattaaatca agaagtatag caattaaagg atattcaaga acttaaaata
     5761 tgatgtttta taaaaattaa tatattgctg aattatttta aaataaggat tttatgaggc
     5821 tgttatataa cgatttgtaa ttgttatctt gacagtcttt tatttttata taagtaaaat
     5881 aagtgtgttt tgctttattt gtatttaaat tgtttttaag ttagaatata aagaaaatta
     5941 tata
//
LOCUS       DAMJVM010000145         5909 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.150, whole genome shotgun sequence.
ACCESSION   DAMJVM010000145 DAMJVM010000000
VERSION     DAMJVM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.150"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(165..1127)
                     /locus_tag="PC523_003318"
     CDS             complement(165..1127)
                     /locus_tag="PC523_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDF2476803.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIVDEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQRITKERNAFRADQFNLEA
                     CNHAHENYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKALKGHKSSIKCYVVEPKTA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQITDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSTERGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            1318..1869
                     /locus_tag="PC523_003319"
     CDS             1318..1869
                     /locus_tag="PC523_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2476804.1"
                     /translation="MSINTIIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SMHTYHNQGEKTLKAVIINYYPV"
     gene            complement(2404..2691)
                     /locus_tag="PC523_003320"
     CDS             complement(2404..2691)
                     /locus_tag="PC523_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476805.1"
                     /translation="MKKFLYALYSFIVIIANFRLKEKNYNFILLAIFFILLLIQNGVL
                     KTGYQNRATFYQDNYFNGEDKNDKIKFMTIKIFIFLSLPLCFNILFDYLTS"
     repeat_region   3262..4410
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3262..3290
                     /rpt_unit_seq="atttacataccacatagttaatataaaac"
     gene            5056..5880
                     /locus_tag="PC523_003321"
                     /pseudo
     CDS             5056..5880
                     /locus_tag="PC523_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
BASE COUNT         2195 a          895 c          630 g         2189 t
ORIGIN      
        1 ttattttatg actaagcata attatctata taaatttatg cttatagtca tgatactaca
       61 tgtttattaa aaaaaaaagt atctcattaa tttttaaaaa tcattttgag atacttttta
      121 caatttaaat ccatatattt aacttaagaa acactgtatc attattataa ttcttcatat
      181 aaatcagtac tcaagtattt cagaccacta tcattaagag tcaaaactat atttttacct
      241 ctttcagtac tctttaataa cttaattgct gcgcatacat ttgctccaga agaaaaacct
      301 gcaaatacac cttctaattt agctaatttt cttgctacat cagttgcctc ctcatctgtg
      361 atttggatat aaccatcaat aagtttcttg tctactagtg gcaagtccat agaatatcct
      421 ccaccttgga ttttatgacc ttggtcagtt atttctttac ctgagtaaat tgctgctgtt
      481 ttaggttcaa ctacatagca ttttatagaa gatttatgtc ctttaagcgc ttttgagcaa
      541 ccagcaaaag tactaccact tccaagaaaa tctacaaata catcaatatt tccatccaat
      601 tgttcccaca tttcttctgc tgtataattt tcatgtgcat gattacaagc ttctagattg
      661 aattggtctg ctctaaatgc atttctttct tttgttattc tttgagcttc aatttctaca
      721 agcgctaaat cttcaccaga tacttgtcca tgaactgagt taggcatttg gtctacaaga
      781 actacttctg cacctaaagc tttcatcatt cttgctcttt ccatagaatt tccttttgac
      841 atacatgcta taaatttata acctttacat gcacaagcta ttgctaaccc tgttccagta
      901 ttaccacttg ttaattccac aactgcttga cctggaacta ataagcctgc gttttctgct
      961 tcggtaatca tttgtaatgc tacacggtcc tttttgctaa atcccggatt taagtattcc
     1021 atctttgcat aaatattacc ttgaactcca aattcatcta ctattttgga taaatgtaac
     1081 atatgagtat tgcctatagc ctctgtaaca ttattaagta cacgcattac aattccccct
     1141 taatctctca actatcatcc gttgaaaaac aattctttat gcagtataat ttatattacg
     1201 gatgtacagt gtatttaacg ttcattatat tatacatttg taaattataa ataacgttaa
     1261 ataaattata tattcgtaaa ttatatttgt caataattta cattttaagg agctaatatg
     1321 agtattaata ctattattgc taaaaaccta aatagattac gtaatgaacg taacttaagt
     1381 ctaggtcaac ttgccgaatt gtctggcgta agtaaagtaa tgctttcgca gattgaaaag
     1441 ggagactcaa accccacagt aaacacaatt tggaaaattg caagtggact aaatgttcca
     1501 tatactgcaa tactggaaca accccaaaat gaaactttta ttgtatcaaa aactgatata
     1561 gatgtacaag tttcagagaa taaagattat cgtctttatt gttattatcc taatacacca
     1621 actagaaact tcgaattatt tcaaatggaa ttagaagaag gtcatagcta tacatctgtt
     1681 ggtcattctg aaaaatcaca ggaatatatt atgattatag aaggtcaatt aaaattagaa
     1741 gtaaatgact ctatttatca acttagagaa aatgactcta tttgtttttc agccgaatca
     1801 atgcatacat atcataatca aggagaaaaa acattaaagg cagtaataat aaattattat
     1861 ccagtttagc catgtcattt aatgatagaa taattatttt ctatacaaaa agataaatcc
     1921 tttgattaag atttatcttt ttcattttat ttttctttat aaattcatta tatatcttta
     1981 cacttttatc aattatgtta taataagctt atataaaagt tttatatttt tgacaaatgg
     2041 agtacaaaat actccttttt cttttttact attgtgataa ttatttagtt agtattaaaa
     2101 tattatcact taaaacaaat acaaaacgtc tcttatatgt acatttttca ttgttgtaat
     2161 aatgatttgt gtatgtattt ctcacaatcg gagtgcttat gcactctttt taaatacctt
     2221 tattaccaca atctaaaagc tattttatta tccattttaa tttattttta agtaatcaaa
     2281 taaattattt tatatttttt cttcatttat atgaaacttt tttatcacta tatgcacttt
     2341 atatttaata taaataacac ataagcttgc ttttatcata acactccaat atttaaatat
     2401 aatttaagaa gttaaatagt cgaatagtat attaaagcat aatggtaatg acaaaaatat
     2461 aaaaatcttt atagtcataa actttatctt atcattttta tcttctccat taaaataatt
     2521 atcttgataa aatgtagctc tattttgata acctgttttt aaaactccat tttgaattaa
     2581 tagcagtata aaaaagattg ctaataaaat aaagttataa tttttttctt ttaatctaaa
     2641 attcgctata ataacaatga aactatataa agcatataaa aattttttca taaaaaacac
     2701 ctaaattcta tatttgtatt ttatattgag ccaaatctaa tttctgtata cttattaagt
     2761 gtagttctaa tttatattgt agttggatta aatatttctt aatttttatt ttcaattatt
     2821 tttataatgt ttatacctct atcaatatta aattttgata tttgtgttgt ttaacattgt
     2881 attactatta aaatcttttt caacaaatac aaatggtata tatactttaa tattatcaat
     2941 actgatagta caagtagtac aaacccttta agtttataag agcatctcct tatatctaaa
     3001 ttatagtatg aatattcaca aatttcaatc tatttataag gtaattattt ttatatctag
     3061 tatcttgtca ttaagtttta ttctatatat ttataaactt ttcaatactt tttataatta
     3121 ttctactttt atattttaat caataagact aaagtcaatg ttatctggta attacttact
     3181 acaaatgata aaaaataatt atattttaaa ggagaaaatg ctatataaat tattggagta
     3241 ttttacctat acttgttgca aatttacata ccacatagtt aatataaaac attcgcaagt
     3301 tcttaatcaa aaacccaatt ttctttattt acataccaca tagttaatat aaaacgctag
     3361 ttgtacttaa ccaatagcca aattgttgac ttcatttaca taccacatag ttaatataaa
     3421 actattatta tttacacatt tactttcaaa acttctttta tttacatacc acatagttaa
     3481 tataaaacta tcctaagaat tctctcttag catttggaca tcttaattta cataccacat
     3541 agttaatata aaacactcct gtagtatttt caaaagttat tgcgctaagc aaatttacat
     3601 accacatagt taatataaaa caagaacgtg cgcttattct tggatatcaa tataaagaat
     3661 ttacatacca catagttaat ataaaaccca tgttcatttc gcctttaaat tcaactttct
     3721 cataatttac ataccacata gttaatataa aacaagaagg tgaaataata ttctatgaag
     3781 ggcaattaaa atttacatac cacatagtta atataaaacc ttgatgttta gctttttgaa
     3841 ataattcaag ttttaaattt acataccaca tagttaatat aaaactttta aataaccccc
     3901 ctccccttct gattttttcg tgatttacat accacatagt taatataaaa ccgccctttt
     3961 cgctattatt ttttttataa cccttcaatt tacataccac atagttaata taaaactgaa
     4021 aaattaaaag aattaataga taagcaatta ataatttaca taccacatag ttaatataaa
     4081 accttctctg aaaatcgtat attctttttt aaaagagtta tttacatacc acatagttaa
     4141 tataaaacga actaacctcg tttcctgtgt taataaacta tgatatttac ataccacata
     4201 gttaatataa aaccaggaca aaatagatta tttgactggt taagaaataa atttacatac
     4261 cacatagtta atataaaaca gtttatttgg ggattagata aaaataaggt agcatcattt
     4321 acataccaca tagttaatat aaaacaatct gtttttcttc ttcttctgca ttatctacaa
     4381 gatttacata ccacatagtt aatataaaac cccaaaataa actaagtatt ttcagtactt
     4441 acacacatac aactttctta aatttgcagt gaaccatgag tagtgcaatt gataacattt
     4501 atcatacacc ctcaacgcct tacattgcaa ctgttaaacc ctactttatc aaaaatatcg
     4561 ctcactgcaa aatttatata tttttattat atcataaaat ttaacctata aaattaaaat
     4621 ggagtaattt atcaatttaa gaatatctgc tagtgcttac tacattggat attctaataa
     4681 gtcttcttct aactggattt aatataaaaa ttctctctaa aaaattaggt catactgata
     4741 taaaaataac tatgaataga tattctcata ttttagataa tatgcataaa aagcttctaa
     4801 aaatataatc aaggctctct ttaaataaca ttcagtttta tatcagtaaa atatcagtaa
     4861 tctcatattt ttcttaaatg tcacttagat gttagttata taataatagt aaacaactgt
     4921 atttcactaa tatttgctat tatccaatag tatttatagt ttagatttga tataatacat
     4981 gtggtaaaat tttgatttgt aacccaaaat ataacctaca tatattaaaa taaatttaaa
     5041 actaaggaga cctatatgtt aaaagtacta aaaggaaata taattttcac aaaaacttca
     5101 gaaactttca cagtatttga aaatagctat attatactag aaaaaggcaa agtaaaagaa
     5161 atatttaaag aaatacctaa ggaatacaaa aatctagaaa taatagatta cactgataaa
     5221 cttataatac ctggtatgaa tgacctccat gcccatgcct ctcaatttaa aaatcttgga
     5281 atggctatgg ataaagagct tttaccatgg cttgaaactt atacatttcc agaagaatca
     5341 aattataaag atatcgaata tgcaacgaaa atgtataaaa aatttgtaaa agaactttgg
     5401 aaatctggaa ctacaagaat agcagtgttt gcaacagtgc ataaagaagc ctctatgaaa
     5461 cttatggata tattcaaaga agttggtctt ggagcatacg ttggtaaagt aaatatgaat
     5521 atgaattgcc ctaatacttt gttagaaaac actcatgatt ctattgctga tacagaagaa
     5581 attataaata agtatagtga tacagattct attgtaaaac caatagtaac acctagattt
     5641 atacccagct gtacaagtga acttttaaaa ggtttaggag atttatgttt aaaatataat
     5701 actcctatgc aatcacactt atctgaaaat tatgatgaga ttgaatgggt tagaaatcta
     5761 gaacctgaat ctaaattcta tggagatgca tataatagat ataatttatt tggtcaaact
     5821 cctactctaa tggctcattg tgttcattgt tctcaggaag aaatagattt gattaaaaaa
     5881 tagctacgca cacttgtgac tttagtcat
//
LOCUS       DAMJVM010000146         5689 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.151, whole genome shotgun sequence.
ACCESSION   DAMJVM010000146 DAMJVM010000000
VERSION     DAMJVM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.151"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            43..894
                     /locus_tag="PC523_003322"
     CDS             43..894
                     /locus_tag="PC523_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HDF2476806.1"
                     /translation="MKNIISAKELISKLEQNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNNTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEKKVNVPSCTGKEIKPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(1022..2635)
                     /gene="ggt"
                     /locus_tag="PC523_003323"
     CDS             complement(1022..2635)
                     /gene="ggt"
                     /locus_tag="PC523_003323"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDF2476807.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLIECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSRKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELKLEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPEHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVESEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2867..4804)
                     /locus_tag="PC523_003324"
     CDS             complement(2867..4804)
                     /locus_tag="PC523_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2476808.1"
                     /translation="MTLFRIAMKNVKNSFFNYLMYFISIVFSVFIFFSFKSIEYNKAL
                     SSLGEKTRMSINTSSILIVAFVFLFIYYSNSFFFNRRSREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLT
                     IFIVIGIRNTIVIRNKRIIELFNKPPEKCRFKKFIKLKGALGVLLITISYLMSISYFI
                     AENIMFSVFILVTIIPGTFLFFSSVMYMIIDAVKKRKGFYYKGQNLIAFSELGFKLKS
                     NSSVLAIIAILIATSVTILGFTISLYYDIDRNISENYKYSYTINLGNSHVNNKIDKLL
                     DKYKKDNKIIFDKTIELIDKDINLEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELISKESVYYVSDSYKKILFKDFEIEKINLHFKDTSECDINLFTVQKTILEPEIN
                     SQATFDLIVVKDKVFNQLKLHGDSRLIRLIDIKNEKKELNLTLKLKNIVSENMEFTYP
                     FNFTSSIESYDNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDRERYIMLT
                     KLGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIVQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMITE"
     gene            complement(4791..5558)
                     /locus_tag="PC523_003325"
     CDS             complement(4791..5558)
                     /locus_tag="PC523_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2476809.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRINEISRELNIESILDKYPSEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
BASE COUNT         1934 a          923 c          627 g         2205 t
ORIGIN      
        1 taaattaaaa taaaaataat ataaacatag gaggtacatt ttttgaaaaa cattatttct
       61 gcaaaagaac ttatatcaaa actagagcaa aatgataatc tagttgtaat agattgtaga
      121 tttgacctta ttaatagaac ttatggtatt gattcttata aaaaaggcca tataaaaggt
      181 agtttcatct tagatataga caaggattta tcatctccta caaaagaaca tggtggtaaa
      241 aatcctttac aagacccttt aatactaaaa gaaaaattag aaaaaatggg tgttgataac
      301 aacactacga ttgtaactta tgatgatgga gatttaaatg gagcttgcag attatttttc
      361 caattaaaac atttgggtct taaaaatgtc tatgtactag acggaggaat aacatctttc
      421 gtaaaagaag gtggagaatt ggaaaagaaa gtaaatgtac caagttgtac aggtaaggaa
      481 attaaaccaa atataaataa tgatttagtt gtcccaatgg agtatgttaa atctaaatta
      541 tacaaaaaag atactgtaat aatagattgc cgtgctaatg aaagatacca aggtttagtt
      601 gaacctgctt actcaaaagc tggtcatata ccaagtgcaa aaaattattt ttgtaaggat
      661 ttaataaaaa gtgactttga aaatggctca cttaaagata tagaattttt aaaaaaattc
      721 tttaaagatt taaataatta tgatgaagta attttatctt gtggttcagg gatatctgct
      781 tgtgttaaca gtcttgccct tagagaactt gatatacctc ataagattta tattggtagc
      841 tttagtgact ggattagtta tgatgacaat gaaatcaaaa ctggccaaga ataaggctaa
      901 caatatataa aatcatagga tagcttaaat tttgtaaagg cgtcagtcat tgtagacttt
      961 cgcctttaat tattatttca tatactcttt aaatttatta tatatatttt agttgataat
     1021 attaatagac tgcaatatga ccatcacatc ttgactctgt cccacagata taactttcct
     1081 gttctgtttt aaatattatt tgacctcgtc ccatgtcgac agtatcatga atcactttta
     1141 tttcatgacc cttttcaatt aatgaatcta ttatatgctt tggcatctct gattcaactt
     1201 ctatttcctt tccttttatc cactgccatc ttggagcatc taacgcttct tgtggattta
     1261 atccaaaatc tatcatattt gttaatactt gaatatgacc ttgtggttgc ataaaagctc
     1321 ccattacacc aaatggtcct attgctttat cttctttacc taaaaatcca ggtattatag
     1381 tatgataagg ttttttaaat ggttttacta cattatggtg ttcagggtct aaattaaaat
     1441 tattacctct actatgtaaa gcaatcccag ttcttggtat cacaattcct gaaccaaaat
     1501 ttatgtaatt actctgtata tatgaaacca tatttccatc cttatctgca gtgcaaaggt
     1561 atactgtacc accacaatat ggctctcctg cagttggata cagagcctct ttattatcaa
     1621 ttaatagact tctttttttg gcatactctt ggcttagtaa ttcctgaatc tttaccttca
     1681 tgtgctctat atcagttaca tatgttttag aatctgcaaa agctagcttt aaactttcta
     1741 taatcttatg tatgtcttct acattttcta tatttccttc tagtttcagt tcttttaata
     1801 tattaagtgc cattaaaact gttattccat gcccatttgg tgggatttca aatattttat
     1861 atcccttata ttctgtactt atcggttcta cccatgttgg ataaaaacac tctaagtcac
     1921 tctttcttat agccccatta aattttctag aatattcatc tattttatca gcaagtcttc
     1981 ctctataaaa actttcagct tgagtatttg ctatctcttc taatgtactt gcctgttctt
     2041 cacatataaa tatatctcca gcctctggag ctttaccatc tttagaaaat gtatcaaacc
     2101 atggcttgaa ctcttctcca ataagttctt tctcatatag ttcatatgat tttttccata
     2161 ctttagctac attaggagat actacatagc cctctcttgc atagtttatc gctggagata
     2221 aacattctat taagctaagc ttaccatatt ttttatttaa ttctgaccaa gctgcgggta
     2281 taccaggcac agttactgct ccaaacccat actttggcat ttccttcata ttattataat
     2341 tttttaattc catattttct ggagcaaagc cactagaatt taaaccatac aatcgttttt
     2401 cttcctctat ccaaacaagg gcatatgcat ctcctccaat cccattactt gtaggctcta
     2461 ctactgctaa tgtagcagct gttgcaactg ctgcatcaac agcatttcca ccctttttta
     2521 gtatttcaag tccagcttga gatgctaatg gtgttgatgt tgcaactgct ccatttttag
     2581 catacacgac atttcttatt gatgagtatt tatgttggta ggcattaaac ttcataaatt
     2641 tcagctcctc gtcatttttt atttgatatt ctgcttaaat ttattatata acatcaagta
     2701 atataactga tacaaaatga tataaaatat agactttttc ctaaccctaa ttttatcatt
     2761 aaataaatat cttaagctca attctagtat tttctatttg cacttaaata aactatttta
     2821 ttcaaaacat acatctaatt attttaactt aaaaataatt tattatttat tctgttatca
     2881 tgtttagagc atattttaaa gttaaaaaat aatatataaa atatcctata aaataaatga
     2941 ctagagttat gataattggt acaagtaatg accttggtat aaacttttgt actatagaca
     3001 tagcaaataa attatgaact gtacctacaa ctaatggcat taaaaatatg acttttaact
     3061 gttttgatat tattttctca atatctttat tatttgctcc aagttttgtt aacataatat
     3121 atctttccct atcatcatat atatttgata attgtttaaa caaaattata ctacctgtgc
     3181 ataaaagaaa tactacagat aaaaatatac ctataaacag cattagacca cttaatttta
     3241 tcagattgtc ataactttca atactggatg taaaattaaa aggataagta aattccatat
     3301 tttcacttac tatatttttt aattttaaag tcaaatttag ttcttttttt tcatttttta
     3361 tatcaattaa tcttattaac ctagaatctc catgtaattt taattgatta aatactttat
     3421 cttttactac aattaaatca aacgtagctt gtgaatttat ttctggctct aagatagttt
     3481 tttgtactgt aaacagattt atatcacact cacttgtatc tttaaaatgt aagtttattt
     3541 tttcaatctc aaagtcttta aaaagtattt ttttatatga atcagaaaca taatacacag
     3601 attcctttga aattaattct tcataattgt tattctgata ttgtcttaat tttttaaaat
     3661 cactttctct gataatatct acagaagttc tatcttctat ataactattt ccatttttat
     3721 agtacatttc aagatttata tctttgtcta taagttctat tgttttatca aagattatct
     3781 tattgtcttt tttatattta tctaataatt tgtcaatttt gttatttaca tgagaatttc
     3841 ctaaatttat agtataactg tatttataat tttcacttat attcctatct atgtcgtaat
     3901 aaagcgatat ggtaaatcct aatatagtta cacttgtagc tattaatatt gctattatag
     3961 ccaacacact actattgctc ttcaatttaa atccaagttc agaaaatgca attaaattct
     4021 gtcctttata ataaaaacct tttctcttct taacagcatc aataatcata tacatcactg
     4081 acgaaaaaaa taaaaaagtt ccaggtatta ttgtaactaa tataaaaact gaaaacataa
     4141 tattttcagc aataaaataa ctaattgaca ttagataaga aatagttatt aataaaacac
     4201 caagtgcccc ttttaacttt ataaattttt taaacctaca cttttctggt ggtttattaa
     4261 ataactctat aatcctctta ttcctgataa ctatagtatt tcttattcca ataactataa
     4321 atattgttaa aaacacagct agtgtctgaa ctaaagcctt tatacttaag ttcatattta
     4381 ctactatcat ttcattcatt aattttacaa gcatcatagt cataagcttt gaaaataaca
     4441 atcctaaaaa tatacctatt agtattgcaa ctatccccat caaaaaagtt tcatataaaa
     4501 atatttttcc tatctgattt tttctcattc caagtaaact ataagtacct acttctcgac
     4561 tacgtctatt aaaaaaaaat gagtttgaat aatatataaa taaaaataca aatgccacta
     4621 ttaaaatcga actggtatta atgctcatcc tagttttctc tccaagtgaa ctcaatgctt
     4681 tgttatactc tatagattta aaactaaaaa atataaacac actaaataca attgaaataa
     4741 agtacattaa ataattaaaa aaactattct tcacattttt cattgcaatt ctaaataaag
     4801 tcatttttgt taactcctcc aattaaggaa actgtattta ctattttatt atagaactct
     4861 ctgttacttt ctccttttac aatttctgta aatattattc catcttttat aaatagtatc
     4921 ctattgcaaa aactcgcact tatagcatca tgagttacca tcaatattgt tttattacta
     4981 tctttgttta atttcaatag acagttcaac aaatctctag cagatttaga atctaatgct
     5041 cctgtcggtt catctgcaag tactaatgat ggttttgttg ctatagctct acatacagct
     5101 cctctttgct tttgtccacc agaaacttca ctagggtatt tatctaatat agattctata
     5161 tttaattctc ttgaaatctc atttattcta ttttctatta ctgatactct ttcatttttt
     5221 aaagaaagag gcaacattat attttctttt attgtaagtg tatccaaaag attagaatct
     5281 tgaaatacaa atcctagatt atcacgtcta aaatccgcca attcattttg cttcatttta
     5341 atagtattct ttccatttat ataaatatct cctgatgaag gtaagtctat tgtagaaata
     5401 atatttaaaa gtgttgattt accagcgcca gatgacccca taataccaac aaattctcct
     5461 ttataaacct tgaaagttat tcctcttaga gcttcatgtt ttgaaccttt tcttccatac
     5521 tcctttttta tattttcaac agataatatt tcttccataa ttcataatca cctttcatca
     5581 taattagtta tttgtcaatt ctcacaaata tattatatat attaaaatag cttttaaaca
     5641 tttaattata ttaatttaag gttacatttt cgtaagtatt aattatatt
//
LOCUS       DAMJVM010000147         5659 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.152, whole genome shotgun sequence.
ACCESSION   DAMJVM010000147 DAMJVM010000000
VERSION     DAMJVM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.152"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            179..958
                     /locus_tag="PC523_003326"
     CDS             179..958
                     /locus_tag="PC523_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDF2476810.1"
                     /translation="MSYKNIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKVFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIINISSQASFQPLPYQNIYSSTKAFVRNY
                     TRALNVELKSKGINAITVCPGWMSTNLFKRGIVVTEKGTKHFPGIVSPDVVAKKALKD
                     TKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
     gene            complement(1910..2728)
                     /locus_tag="PC523_003327"
     CDS             complement(1910..2728)
                     /locus_tag="PC523_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2476811.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IESTIKRIGGNDIMKNYDVTIKNFASKYMMTLRMVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPRHSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGTWISDNDYDVDGPMFNIYHVSPGNDNNPDNWVTEVCFPVKKK"
     gene            2896..3477
                     /locus_tag="PC523_003328"
     CDS             2896..3477
                     /locus_tag="PC523_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2476812.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCIANGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEGVDVR"
     gene            3474..3668
                     /locus_tag="PC523_003329"
     CDS             3474..3668
                     /locus_tag="PC523_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476813.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(3832..5574)
                     /locus_tag="PC523_003330"
     CDS             complement(3832..5574)
                     /locus_tag="PC523_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HDF2476814.1"
                     /translation="MVKSKKKIFVSLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSEEFYSNEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLSKSTVSIDGIEIDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDICEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLDDINKLFFDLEEEYGVSNNILKEKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQGDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQDGNLEEISSTLYD
                     YFGIDNPLELKNAS"
BASE COUNT         2087 a          695 c          772 g         2105 t
ORIGIN      
        1 taatattact tttatactat aattaacact gattctataa tattattaac attacttctt
       61 atgctatata acaattattg aaaatgatat gaatataaca tctgaataga gggtgtttta
      121 tttgtaaaaa ataagtttac agtttgagcg tataaactta tggtaaagga gaataattat
      181 gagttataag aatattgcaa ttataacagg tgctaatagt ggttttggta aagagtttct
      241 aaaattatta ataaatgaag aagaaataac agaaatatgg gcagttgcaa gaaacaaggc
      301 gagattaaat caattagtag atgaatttgg aagtaaggta aaagtttttt caaaagactt
      361 atcaaagata gaagaagtaa aagaaattgg tacattttta agtaaagaaa atgtatgtat
      421 aaaatacctt ataaataatg ctggttttgc aaagttctgt tcatacaatg atttaagtat
      481 agatgaatct attaatatga ttgatttaaa tattagtgct gttgtagcat tggggcttat
      541 atgtattcca tatatgcaaa agggaagtca tatcataaat atttcttcac aagcatcatt
      601 ccaaccattg ccataccaaa atatttatag ttcaactaaa gcatttgtaa gaaattatac
      661 aagagcatta aatgttgagc ttaaaagtaa agggataaat gcaattactg tatgcccagg
      721 ttggatgagt acaaatctat ttaagagagg tatagttgta actgaaaaag gaacaaaaca
      781 tttcccaggc atagtgtctc cagacgttgt tgctaaaaaa gcattgaagg acacaaagaa
      841 gaataaggat atttctgtat atggtataaa tacaaaatcc agtcatttac tagcaaaact
      901 attgccacaa aagatgatga tgaaggtttg gttaatgcaa caacatatta aggaataaga
      961 gttttggtaa ttaagtatca gttagagatt caatctgtta caaattatga gaatttttgt
     1021 tgagtaaata ataattgtga caaaacggct tatctagtgc tgataagccg tctacaatgt
     1081 atataaaata tatttttaat ttcaaagtta ttacaccacc ttcttaaaat attagattat
     1141 ataaataatc tacaatcaca actgattaac aaaattatga gttaataaat tatatcacat
     1201 gcaaatatga aagaatagta tgaaattttt ataaacatat attaaatgtt tctcaatgaa
     1261 attgactttg tatatgaact gaatgactta ttcaatatgg ataagacatt tttcttgtaa
     1321 taagatatga ttgtcctata aggtttatgt aattttgata taatatattg attaacagca
     1381 tacatgttat tggtgaacat atttctatag aatggaatat ataatttttt atatttatca
     1441 ttgtatcatt gtctaaagaa agttaaaaag agtagagcac aaaggaatta tttgtacttt
     1501 actcttttat tgttttgtta aaaatactaa tttatattag tgattaaaat atatttaact
     1561 agagtataag gatagtatat aatcttataa ttgatttatc tgacaaatta agataatata
     1621 ctaatcaaat tctggttatt taaacataaa atcataattg atttatctga caaattgaga
     1681 taatatccta attaaattgt aggtatttaa acataacatt ataaataatt tatctgacaa
     1741 attaagataa tatactaatc aaattctagt catttaaaca taatatcaca agtgatttat
     1801 ctggcaaatt aagataatat cctaattaaa ttgtaggtat ttatacataa cactaaaagt
     1861 aatttgtcta acaaattaaa atattatcca attaatatat aagataaaat tacttctttt
     1921 taactgggaa gcatacctct gttacccaat tatcaggatt attatcatta ccaggactta
     1981 catgataaat attaaacata ggaccatcca catcgtagtc attatctgat atccaagtac
     2041 caatagcttc acaagcatct gcaacttgat tgaaatttcc attaacaatt gcagttgcag
     2101 cagtcacact tggaacagtc ttaaatttaa catgctcagt atctttatac tttccagata
     2161 cagcaacttg tacttcaaca tctacataat cttctttata tccagtatca taaaagactg
     2221 ccttagaatg tcttggtggt tcaatttgaa cattctggtc ttttgtctct aaaaatgctt
     2281 gatgccataa cattccttcg tcttgataag ttggtataac cattctaagt gtcatcatat
     2341 attttgatgc aaaattcttt atagttacat cataattttt cataatatca tttcctccaa
     2401 ttcttttaat agtactttct atttttaata gttttttctg tgtatcttct aattgttctt
     2461 ttacttgaga atggtggata tttagatatt ttataagact ttctttatcg ttatattctg
     2521 tcaaaatttc ttttatagaa tacaagccaa agcccatatc ttttagtgca tggattctgt
     2581 ttgttgtaga aagttggtct gcgctatagt atctatatcc attagtttta ttcgtatgag
     2641 aaggagttaa aagccctatt tcgtcataat gacgtaacat acgaatacta attttactta
     2701 gttttgaaaa atcgcctatc ttaaacatgt ttttgtctcc tttataaagg tacttttagc
     2761 ttaatattaa tataaaccat atcataatgt tagagtcaac taaaattata attaaattat
     2821 attgacatat gatgaaaaaa atattatact gaacatgttc agaactaaaa atgttaattg
     2881 ttttggaggt ccattatgaa aagtaatgat aaagatgcta gagaacgtat aattgaagtt
     2941 acattgaatc ttttaaatga agtagatgat attgaagaga ttacagttag aaaaattgct
     3001 gagcgtgcaa atgttggagt tggtttaata aattatcatt ttaaaacaaa ggataatttg
     3061 ttgagtactg ccataggaga tgtgatgtca aatataatag cagagctata tgacgatagt
     3121 gtatatactc taagacctat tgaagatttg aaaaatttat taaaaaaatt gtgtgatact
     3181 ggattacatt atgaaaaagt gcttcctttt gtgttgaatc aatgtatagc caatggagat
     3241 atgcaagcag aacttgatat tgtccctatg cttcgtaaaa tttttggaaa taaaaaagat
     3301 gaaatgagtc taagaattat tgccctgcaa attattttac caatacagat ttctgcttta
     3361 tctacagaat catttcagtt atacagtggt ataaatataa agaataaata tgagcgagat
     3421 aaatttattg atattttaat agaaaatatt attggagagg gtgttgatgt aagatgaaaa
     3481 ttataactat tggttcaagt cttatcactg ttttactttt tctttcaact atggtttgtg
     3541 ggttctggat taagaataat aaggtaacag atgcaagttc tattaagttc catatgaata
     3601 gtgctatttt tacagggata tttttattga tttccacaat ccttttaatt atatatataa
     3661 aaaaataata atattattaa tagataaagt atcagaaatt ataaagacaa gagtcttaaa
     3721 aatttctgat actttaccat ataattttat taaacattaa ttgtgtgtga aaaaatatca
     3781 cttatttcaa tctaatgcta aatcttaaga gtaagcataa agtattaaaa attaagaagc
     3841 attttttagt tcaagtggat tatctatacc aaaataatca tataaagtag agcttatttc
     3901 ctctaaattt ccatcttgga aaggtaattg taaattcacc ttttctacca catccttaaa
     3961 tccagcatca gtattttgat ttaaaaaatc catatataca tcaacagctt ttctgtaatc
     4021 accttgtgat aattgccata tctgaagtga tacatcagat gcaagaccat aactcaagta
     4081 ataaaaagga acttggaagt tatgagatac aagtatccaa aaaggagcat ttttttcttt
     4141 aagtatatta ttggaaactc catattcttc ttccaaatca aaaaacaatt tattgatatc
     4201 atctagactc atatatggat tcttatatat aatttcttga aattcatcat aaagacaagc
     4261 atcaataata gtgtttaaaa caatcgataa gtgttctttt tttatagctt cagattgttt
     4321 cccaaaaaaa tcatcaaaat atttgtaaaa tagaatttct aaagatgttg aatgaacttc
     4381 acatatatct atacttggtt gcatcttatt atttaagtta atactgttgt agtaattata
     4441 aaaatgacca aattcatggg caaaactagt aacacctaaa aaagaattat cccatgtatt
     4501 aaataagaaa ggagctttat acttatttat ataagtggta tatcctccag gtgatttgtt
     4561 ctttccagaa gaataatcgt acaagtcata ttttttcata tagtcaaagc tctcttttag
     4621 ttttggagat atatcttcaa gcaccttatc aaactttcta aatgagcgat ttttatatac
     4681 tttaatataa atagaagaat cagaaggttt agatgaaatc tctctatata atggaactat
     4741 gtaatctttt acatcttgtc taaatttttt agcttcctct tgagaataat ctttgtttag
     4801 gttcatatag gcataatcta tataattttt aaaaccttgc ttacttgcta tttcagttct
     4861 tgtctgtata agttctaaga atatgttacc aaatataggg ttgtattttt ttaagtaatc
     4921 tgaataaatt ttaacatatt cttcagggct aagatttggt cttgacaagg cttcttcaaa
     4981 gtctatttct ataccatcta ttgatactgt agatttagat aacaagtctt catattttac
     5041 aactaggtct tgttctttct taagtaaacc ttcaacttct ttagaattaa gttttccaat
     5101 gttctttaga taattaaatg tatcttttcc aagttctttt tctaattctt tagagaattt
     5161 agaagttaca aatctatctt gaaagtcttt gtatgacatg tcaacatcta ctgatttact
     5221 cataagatac ttgtattcat tagagtaaaa ttcttctgat gaatctatgt tatttctaat
     5281 ttttgctata gtaagtgtac tgtaaaaatc ttgataaatt ttatcaactt cattaaataa
     5341 atttaattgt tctttagctg ttttagctgt taaaagtttg tcgttacatt tgttaatgtt
     5401 ttcgcaaatt gactgtatat taggacgctt atattccatt ttttcaaaag gaatagttcc
     5461 tctaacagaa tctttttttg tatcattagt ataatttctt atgtatgatg caaatataat
     5521 aaaaactgca attaaaatta gagaaacaaa tattttcttc ttggatttaa ccataaaaat
     5581 cctccaatca tggatatagt tattaattat tttactatat taaatataaa aaatgcatgt
     5641 taagaaaatt taacataag
//
LOCUS       DAMJVM010000148         5357 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.153, whole genome shotgun sequence.
ACCESSION   DAMJVM010000148 DAMJVM010000000
VERSION     DAMJVM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.153"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(214..1398)
                     /locus_tag="PC523_003331"
     CDS             complement(214..1398)
                     /locus_tag="PC523_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2476815.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMENNGIKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCKYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMVKLVNAVPIFIDTKKENDFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYSGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKAIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSTDIDNMVKTYKSRRDLIKSKLDSIENIGYVNPNGAFYAFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLNKLKEFVYKI
                     MA"
     gene            1771..4122
                     /locus_tag="PC523_003332"
     CDS             1771..4122
                     /locus_tag="PC523_003332"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HDF2476816.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDEELVNKMQNFAFEESYKETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDELGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRDKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4144..4683
                     /gene="nrdG"
                     /locus_tag="PC523_003333"
     CDS             4144..4683
                     /gene="nrdG"
                     /locus_tag="PC523_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDF2476817.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEVIAREAKTNGLDVWSYTGF
                     TFEQLLDKRNSAYFKNLNLLKQIDILVDGKFVAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2091 a          593 c          849 g         1824 t
ORIGIN      
        1 taataaattt agaatttaac ctatatagtt taacttaata atttaatgaa aaaaataaaa
       61 gacttactga tttttaaatc tataagtctt ttatttttta tttaatattt aaattaagtt
      121 gttgaatata tcttatatta cttactttat atattgatta tcattaggtt aagacccata
      181 tattgtatca attactattt atttaaattg ttattatgcc attattttat atacaaattc
      241 ttttaattta tttaatccag ataagaatgt attttcactg caagcataag atatacgaat
      301 atatttatcc ataccaaatg ctataccagg aacaactgca acattatatt cttctaaaaa
      361 ttgattacaa aattctatag aaaaactatc tttatattca aatttctcag atactttaga
      421 aaggtctata aatgcataga atgcgccatt tggattcaca tatcctatat tttcgataga
      481 gtctagtttt gatttaatta aatctcttct agatttatac gttttaacca tattgtctat
      541 gtcagttgag caatctttca aggctccata agctatgtat tgtgctgtca aacttgggtg
      601 agatataaga tgtccttgta tagaactcat tgctttagca atcgctttat tagatgcagt
      661 ataccctaat cttaatccag tcattgcagc tgacttagaa aaaccattta tagttattgt
      721 tatatcttta gcttcttcgc ttaatgatgc aattgatgta aattcgccac tgtagcatat
      781 tttttcatat atttcatctg ctaaaatata tattttattt tgtatacaca catctactat
      841 ttctattaat tcatcttttg tataaacact accagtagga tttgaaggat tgtttattac
      901 aagaattttt gttttatctg ttatagattt ttcaagttct tcttttgtca gtttaaagtc
      961 attttctttt ttagtatcaa taaaaattgg aacagcatta actaacttaa ccatttcagg
     1021 ataactaacc caataaggtt ttggcaataa gacttcatct ccttcgtctg ttaaagctag
     1081 taaagtattt gttattgagt tttttgctcc gcttgataca actatttcgt ctattgaata
     1141 tttacagttg ttttcctcaa taagtttttt acatatttct tctctaagta tttttaatcc
     1201 tggaactaaa tcatatttag tgtaatcttt atttaaagaa tcaataccat aagattttgc
     1261 gttattaggt acattaaaat caggttctcc tatactaaga tttattactt taattccatt
     1321 gttttccatt tctttaactt tagaactaat acctattgtg taggatggtg ttataaagtt
     1381 tagtcttttt gataacataa aatgcctcct tttaaattat tatttatatt atacgacaat
     1441 tttaaagaat ttacataagg aaatttgtag atatatacat taatatatat aatagaatac
     1501 actttttgcc taaaattagt gtaaaaaaaa gagaaaaaaa ttttgacttt caaagttgtt
     1561 agaaacgcta ctgttcaaaa tatccacata gtatttgcaa tgatttgcgc aagtttgact
     1621 attatgtaca aatttggtaa tcttaatata gtaattatta agatagtccc tttaacttag
     1681 ttgaagggga tttttttaga cttaatatac tacatatagt ggtatgtgga ttaacaaaat
     1741 acaatatata gtaacaggag gtttttttaa ttggttgaat tcgtaaaaaa aagagatgga
     1801 agagtaattc catttaatga agatagaata acaagagcta tatttttagc agcaacgaat
     1861 gttgcagaaa gagaaggaat agtacctgat tataaattat ctgagcaatt aactcaagaa
     1921 gtaataaagt ttttaaatca taaatactca gaatcagttc caagtgttga agatatacaa
     1981 gattctgtag ttaaggttct tatagaaact ggccatgcaa aaactagtga agaatatata
     2041 atatacagaa ctgaaagaag tagaataaga aattcaaaaa caagattgat gaaggcaata
     2101 gaggaaatta cctttgaaga tgcagaagat gctgatataa aaagagaaaa tgcaaatatt
     2161 aatggtaata cagcaatggg aactatgtta caatatggaa gcactgtatc taaagaattt
     2221 tgtaaaactc atatattaaa accagaacat tcttttgctc atgataatgg agatatacac
     2281 atacatgata tggatttttt aaatatggga acattaactt gttgtcaaat agatgttaaa
     2341 aaactattca atggtggatt ttcaacagga catggttttt taagagagcc acaagatata
     2401 ataagttatg gagcattagc tgcaattgct atacaaagca atcaaaacga tcaacatgga
     2461 ggtcaaagta taccattttt cgattatgga ttggctgaag gtgtatataa gacatttaag
     2521 aaattctaca taggaaattt agctaaagcg ttgaagttat ttaaaggaat tgaaaataat
     2581 gatgtgataa agaacatagt atataataca gagaaagaaa caaatcaaaa agttggtctt
     2641 aagagagatg aattgtatct aaacttagaa aaagagaaat taatacagac ttttgatata
     2701 gatgaggaac ttgtaaataa gatgcaaaac tttgcatttg aagaatcgta taaagaaact
     2761 gataaaaaaa catatcaatc tatggaagca tttatacaca atctaaacac aatgcactct
     2821 agagcaggtg cacaggtacc attttcaagt gtaaacttcg gaacagatac ttctgaagaa
     2881 ggaagaatgg taactaagaa tcttctatta tctcaggaaa gaggtttagg taatggagag
     2941 acaccaattt ttccaatttt aatatttaag gtcaaagaag gcataaattt aaatcctgaa
     3001 gaccctaatt atgatttatt taaattatca tgcagagtat cagctaaaag actattccct
     3061 aattttagtt ttttagatgc accttttaat gcaaaatatt ataaaaaagg tgaaccagat
     3121 acagaggcta catatatggg ttgtagaaca agagtactta gtaatgtgtg tggttcagaa
     3181 accgttagtg gtagaggaaa catatctttt actacagtaa atttaccaag attgggtata
     3241 aaacatggta ttataaataa tgaaaaggct aatttagatg gtttctttga agaattagat
     3301 gagaagataa acttaattat agaacaatta ttggaacgtc ttgaggttca aggaaataaa
     3361 aagatgaaga attttccatt cttaatgggg caaggtgtat ggaaaggttc tgatgaatta
     3421 ggtccagaag atacattaaa agaagtaata aaacaaggaa cattgacaat tggatttata
     3481 ggtttagcag aatgtcttat tgctcttata ggtaaacatc atggagaaag taaagaagct
     3541 caagaattag gcttaaagat agtgtcacat atgagagata aaatggatga agcaacagac
     3601 aaatataaat taaatttctc tttaatgggt actccggcag aaggtctttc aggtagattt
     3661 actaaaatag ataaaaaagt ttatggggaa attaaaggta ttacagataa agaatactat
     3721 actaattcat tccatgtacc agtttattac aatataagtg catatgataa aattgagata
     3781 gaagctccat accatgaatt gactaatgca ggtcatataa catatgtaga gttagatgga
     3841 gatccatctg ataatctaga agcttttgaa actgttataa aggctatgaa agatttaggt
     3901 ataggatatg gaagtataaa tcatcctgta gatagagacc caatatgcgg tttttcaggt
     3961 gtaataacaa gtaatatatg ccctgtatgt ggaagaaatg aagatgaaag tgacatcaaa
     4021 ttcgaaagaa taagaagaat aacaggatac ttagttggta cagttgatag atttaataat
     4081 gctaaaaaag cagaagtaag agatagagta aaacatagat agttttattt gggggaataa
     4141 gttatgaaaa taagaatgtc atctactata agttatgaca gcatagttga tggaccaggg
     4201 ttaagaatgg tgatatggac tcaaggatgt attcataatt gtaaagagtg ccataatcct
     4261 caaacgcatg atttatgtgg aggattttat atggatacag aagaaattat aaataaagta
     4321 aaatcattga aattacaaaa aggtattacg ctatcaggag gagaaccatt tttacaacca
     4381 gaaccattag aagtaatagc gagagaagct aaaacaaatg gactagatgt atggtcatat
     4441 actggattta cgtttgaaca actgttagat aaaagaaata gcgcatattt taaaaattta
     4501 aatttactaa aacaaattga tattttagtg gatggaaaat ttgtagctga aaaaaaagat
     4561 ataagcttaa aatttagagg ttcatcaaat caaagaataa ttgatgttca aaaaagtttg
     4621 aaatataaaa aagtgttttt ggttgaacaa tatatgaaag atgatttgtc tatagcagaa
     4681 taaaatataa ataaacagga gtaaatatat gcttatattt actcctgttt atttgcatat
     4741 aatatttcaa gcttgtatga tgtaacaatc aaaccaaaat acttattgta tatctgaaat
     4801 taagtttttt atagtttaga ggtgttttat gtatattaca aagggtatac catacattat
     4861 ttaatcgatt cttatgccaa gaaacaatta atttacagaa atttttaatt gttacaatat
     4921 aaatatttag ataagttata aaatgttgat aaataattta gaaagtttat taatccaata
     4981 ttatgtatgt ttatattaag taatcaagta gtctaaaaaa tgaaaaaggt ttaaaataag
     5041 tattttaact aaatgtataa gagttaaatt tacatataag tatagattag atatataaga
     5101 aataattatg taatatctat ctttgtaaaa aaatattaaa ttttgtaatt tgtggataaa
     5161 tatgtttata atgtggataa tatttaatgt aataagttaa agcaatatga atcaactgtt
     5221 tataatgtgg ataatattat aaatatatga ataattgtag tatttaaaaa caaaaaaagt
     5281 taaaaaagtt gttgactcta aaagaaaaag ttggtatagt aatacttgtc ctcaagaaaa
     5341 ggacaaacac ataaagc
//
LOCUS       DAMJVM010000149         5131 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.154, whole genome shotgun sequence.
ACCESSION   DAMJVM010000149 DAMJVM010000000
VERSION     DAMJVM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.154"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            304..1038
                     /locus_tag="PC523_003334"
     CDS             304..1038
                     /locus_tag="PC523_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2476818.1"
                     /translation="MNLTKGLDVLKISSNVFGEDKVMYIPVIYTEDEATLIDTGLPGQ
                     GDLIVDALNKSNTSFDKLKNIIITHHDIDHIGNVNYLREKSKNNIKVYAYKSEVSYIT
                     GEETPFKLYMLEQMVDKIGDKMLSMLNVMGLGFKSSYTKVDVSLDNHEFLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQIDNGELKPVDVMHSNKQELKDAIKNISNYD
                     IEAIVFSHGGLYQENIIETLNNIIIE"
     gene            2070..3326
                     /locus_tag="PC523_003335"
     CDS             2070..3326
                     /locus_tag="PC523_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF2476819.1"
                     /translation="MITKFKTLIWIKILIRIKSVYKAYGGLFLLGIIGIPIGAIIGLI
                     DTVFGTVLLKITDIRETYPMYLIPFLSVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLIPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWIGKRLPIKNASSIFLV
                     TGMAAGFAGLFGTPIAAILFAMEVLVAGSLEYQSLFPAFIASFTASEVTKALGLEKFS
                     FALSSKVVFDLSIFWKFIALGIIFGMVGGAFAWCLKLSKRKICNRLKNPMIRIAIIGA
                     CISILFLLFDKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFVLTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYVFNMDKSIYSLQKISTKQ"
     gene            3369..3750
                     /locus_tag="PC523_003336"
                     /pseudo
     CDS             3369..3750
                     /locus_tag="PC523_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
     gene            3941..4321
                     /locus_tag="PC523_003337"
     CDS             3941..4321
                     /locus_tag="PC523_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2476820.1"
                     /translation="MYLKKLSKLELVIMKFIWNLDIKTNSCEIIDYMKEEHNLSEKVA
                     LKTLSKLSKKRFLYVQETDKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEDLTEEKIKSLENWVVNWEEACI"
BASE COUNT         1966 a          493 c          807 g         1865 t
ORIGIN      
        1 tatagttttt ctatatttat attttctata ttactttacg tttaacaacg tacaaattat
       61 taaaaataaa atagttatta gaaaaaaata aaagtaattg caaagattgc aattacttac
      121 ttctatatta attactatac cataaaataa caaaaaaatc aagtcgagta atccctcaac
      181 ttaagtgata tactaggtat taataatttt taattaaaaa aatataacta gaggcaaatc
      241 tatattttct caatttcttt aattaaattg acctctaatt aagtaattat aaggagaaaa
      301 attatgaact taacaaaggg attagatgtt ttaaaaattt catcaaatgt atttggtgaa
      361 gataaagtta tgtatattcc tgtaatctac acagaagatg aagcaacgct tattgacaca
      421 ggtcttccag gtcaaggtga tttaatagtt gatgctctta ataaaagtaa cacaagcttt
      481 gacaagttaa aaaatataat aatcactcat catgatatag accatatagg aaacgtaaat
      541 tatctaagag aaaaatctaa gaataatatc aaagtatacg catacaaaag tgaagttagc
      601 tatataacag gagaagaaac tccttttaaa ctttatatgt tagaacaaat ggtagataag
      661 attggtgata agatgcttag tatgctaaat gttatgggac ttggatttaa atcatcatat
      721 accaaagtag atgtatcatt agataatcat gaatttttga atctaggtga ggaaatagaa
      781 gttatacata caggaggtca tactagaggt catatatgtc tttatttaaa agagtctaaa
      841 gtacttattg ctggagattt gctacagata gataatggag aacttaagcc tgttgatgta
      901 atgcacagca acaaacaaga gttaaaagat gctattaaaa atataagtaa ttatgatata
      961 gaagcgatag tttttagtca tggtggatta tatcaagaaa atataattga aacattgaac
     1021 aatataatta ttgagtagta tttattatat gattttaata atctacaata atttcttaaa
     1081 ttacataacg ttgtatttag gtgtgaaaaa ttaaaatcaa atataaataa taaaaattag
     1141 attgtatcaa taaaaatatc cttataattt atttatttgg attctaagga tattaaatct
     1201 attacaagta tatatataga tgtagttaat tacactacaa gttaaagaat ctaaaccaac
     1261 aattaaaaga aatcaaaata taaatccaat caattaaatt cttgacatat ataaattgtt
     1321 tttatccttt tttgggtgtc gataataatt tttaaaattt aatgtcaagt aaacacaata
     1381 aaacacagag cttattctaa aattatggtt tttattttat gtatacagtt atagtattgc
     1441 aagtagttta caaaatggtc tatatgtgtc aaattgaaag tcttgtaata cttaagttga
     1501 aaaatattta acatcatagt ataatagggt agaaaatact ttaaatcaac tttagaatac
     1561 aatattataa ataatcactc agatagatat tataaaaata aaataaattc tggaaaaggc
     1621 ataacatgga tgtactggat aatttagatg ataaattgta aattgtatta tttacaacat
     1681 aaagaatata acataaggga taagattgac atataaaaaa tgatataata attatgggaa
     1741 tgaggttctc ccttggatga taatatccta aactgccaat gagctgatga cttctatgat
     1801 atcatagatg ccaccagctt tttttgttgt ttaaattgat aaaatggagg tgaaatagtt
     1861 actaaattct tattgtttga atttgaataa atatttaaaa gatagaaaaa atggcatttt
     1921 atatgctaat tactttgtta gtatagagat taagagtgtg gctatatgct attaattaaa
     1981 aaaaacaatt attatagaag ccacattgcc tatagttgat gcaataatat tttcaaaata
     2041 tcttataaag caggtagaaa ggaagtaaaa tgataacaaa atttaaaact ttaatatgga
     2101 ttaaaatatt aataagaatt aaaagtgttt ataaagcata tggaggacta tttttgttag
     2161 gaattatagg tataccaatt ggtgcaatta taggtcttat agacactgta tttggaacag
     2221 ttcttctaaa aattacagat attagagaaa catatccaat gtatttgata ccatttttat
     2281 cagtagtagg tgttgttatt gcttactgtt attttaagtt tggaggaaag agtagtaaag
     2341 gaatgaatct aatatttgag gtagggcatg gagaagaaga aattattcct ttaagattaa
     2401 ttccatttat catatcaggt acatggttaa cccatttatt tggtggtagt gcaggacgag
     2461 aaggagttgc tgttcagatt ggtgctactt tttcacattg gataggcaag aggttgccta
     2521 taaaaaatgc ttctagtatt tttctagtaa ctggtatggc agctggtttt gcaggattat
     2581 ttgggacacc aatagcagca atcctttttg ctatggaggt tttggtagct ggttcactgg
     2641 agtatcaatc gctttttcct gcatttattg cttcttttac agcaagtgaa gtaacaaaag
     2701 ctttaggatt agagaaattt tcttttgcac tttcttctaa agttgtattt gacttatcaa
     2761 tattttggaa gtttattgct ttaggaatta tttttggaat ggttggagga gcatttgcat
     2821 ggtgtcttaa attgtcaaaa agaaaaatat gtaatagatt aaaaaatcct atgataagga
     2881 ttgctattat tggagcatgt attagcatat tattcctttt atttgataaa gggagatatt
     2941 ctggtttagg gacgaatcta attcaaaata gtttttatgg tggagaaatt tattcatttg
     3001 actggctgtt aaagtttgta cttacaatac tgactttgtc ggcaggtttt caagggggag
     3061 aggttacacc tttgttttct attggagcta gtcttggtgt attactagca ggatttttta
     3121 atttaccaat tgaattagtt gcagcacttg gctatgctag tgtctttggt agtgctacaa
     3181 atactttttt tgcacctgta tttataggag cagaagtttt tggatacagc tatttgcctt
     3241 atttctttgt agtatgcgct atttcatacg tatttaatat ggataaatct atttattcgc
     3301 tacaaaaaat ttcaacaaaa caataaaaaa acaacataat atgttaatgg aatctgttta
     3361 gcagtatttt taagaaagtc catatgagag attctgctaa aagtgtttta cttttgggta
     3421 tattattttt aatgataagt ttggagaatg atttagatgg aattgtgtca ttttccggat
     3481 tattagcagt tatgagtatt cttatgtcct attaagacaa aattgatatt aaaagaaata
     3541 ttattctgta tgatgggata tattccaaaa gctaaagtac agatagcaat aggaagtgta
     3601 ccacttgcaa tggagatggt atctgggcaa tttattttga cattagcagt tctatctata
     3661 cttataactg tgcctcttgg aacttttgga attgatacaa cttataaaaa attattaaca
     3721 tctgtaaaag gtgacagtaa gcaaaaataa agattatttt aagtggtcaa ttttaaaaaa
     3781 agaaccatat tatgaataca atgtcagaaa atcatttgag agattaaaga agatagaagt
     3841 tcagtatgga ttttaaggta tgtgatgatt attagaattg attttgtgaa attattattg
     3901 taatatttaa ctatatagat aaagaagcgg agtgataact atgtatttaa aaaaattatc
     3961 gaaattagag ttagttatta tgaaatttat ttggaattta gatataaaaa ccaattcatg
     4021 tgagattatt gattatatga aagaagagca taatttatca gaaaaagtag ctttaaaaac
     4081 tttatcaaaa ttatcaaaga aaagattctt atatgttcaa gaaacagaca aatgtatgta
     4141 ttatacagtt gcaataaaag aaaaggcata tcttgagttt atatcgagaa atgtgcaaaa
     4201 tcttttgaag aataatttta taaggaattt attagtgtca tttcatgagg aggatttaac
     4261 agaggaaaag ataaaatcgt tagaaaactg ggtagtaaat tgggaggaag cttgtatata
     4321 atgttattat ttttattaca atttagataa ttttggtttt gtaagctctg caactatttt
     4381 tagatggttg cagagcttat ttttattttt aatttcctta agtaatactt aaaagtaaac
     4441 gtatttatat ttactaactt caatcacttc taatacttac gttacttttt aaaagtattt
     4501 tgatatgtca tctctatagt aatctaaaac ttgtttctat tatttatatg aagtttttaa
     4561 ttattagaga tgattatatt taaaataaat tatcatataa attatgctaa cttattagac
     4621 ttgtatttaa aacatatttg gcttaaattt aaaaaattca agttttgcat agactaaaaa
     4681 aagtttttat gaatgatata tataatattc attcatttga ttaaaaatag aaaaatatta
     4741 aataattcta ctgcggtagt gtgttaaaaa aaagaataaa aaatgttaat aaataaacaa
     4801 gaattatatt aagagaaggg aattttctaa aatacaaaaa ggtatctaga tttcataaaa
     4861 aatattattt tgctcttaaa aatatttaga ttggaaaaat tttaatttaa tgattgatta
     4921 agttaaaaat gttcatggaa taaattttat tttatttatt gactaaatta taaagtttat
     4981 ataattattg aataatcatg tgattgttac ttaaaaattg atatatttta aaatctagtt
     5041 tgggtttcaa taagactgaa aattaagaaa aaagaagtga atatatagaa ttatatcata
     5101 taggtttttt tattttactt caataaaacg a
//
LOCUS       DAMJVM010000150         5058 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.155, whole genome shotgun sequence.
ACCESSION   DAMJVM010000150 DAMJVM010000000
VERSION     DAMJVM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.155"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            317..3151
                     /locus_tag="PC523_003338"
     CDS             317..3151
                     /locus_tag="PC523_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2476821.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKIGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKIPGKPILFLHASIIENLIGRTLNSNEFSINNCNELLKSSKN
                     DTEDIFSTLLGYDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIRVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSEIDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     STYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     AIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEISYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNNKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     LTIIELIDEELKSKFKLGYILHCSFMIERCFKNTTFPYNNITEIISKNHELYSLIREA
                     FKPTEAYFNIFIPDEEIAYIIDMINEYLNK"
     gene            3194..4438
                     /locus_tag="PC523_003339"
     CDS             3194..4438
                     /locus_tag="PC523_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2476822.1"
                     /translation="MEDEILKGKIKQLTILTLIFIFITPGFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAIDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAALAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENISTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMIPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSASLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKIKVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVNVKSESNIGCIPLKDK
                     IKIRVAKINRKLDVKNSVCFKV"
     gene            complement(4895..>5058)
                     /locus_tag="PC523_003340"
     CDS             complement(4895..>5058)
                     /locus_tag="PC523_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476823.1"
                     /translation="NVSVLVKAGAVSETTATDSVANATFEVKKHVAAPISTIGKISSG
                     LSSLFNILR"
BASE COUNT         1964 a          659 c          751 g         1684 t
ORIGIN      
        1 ttatcatttt tttcatatat atcgactata catcaattat atcaaccata tattaataaa
       61 tgatattaca taaattgaaa tatattgaaa aacttaaatt tagttatgta acaacataat
      121 actctaattt aatctataaa aatctatacc taatataatg caactcttat tttaacacca
      181 tctattacac aaaacaacat attaaaaagt aaaatacaaa atatccacta taaaccttat
      241 tttaatacta aatatatatt ataatgtatt taatatagct taaatatatt ttaaacataa
      301 agaaaggaga cgatatatgc taagaaaaga taaagttcta ttatcactac ataaattatg
      361 tgaaaatatg actttaaaag accttcagtc agataaaata ggattcacta caatagaaat
      421 tgctaaccat tcatctcttg atagaagtaa taccagtaaa gaattaaata acctgtataa
      481 tgaaaaaaaa gttctgaaaa tacctggtaa gccaatttta tttctacatg ctagtattat
      541 agaaaattta attggacgta ctttgaacag taatgaattt tctataaata attgtaatga
      601 actacttaaa tcaagtaaaa atgacacaga agatattttt tcaacattac ttggatatga
      661 ttctagtctt aaactaccta tagaaaaagc taaatctgct atactctatc ctccaaaagg
      721 tcttaataca ttactcatag ggcctactgg tgttggaaaa agtacatttg ctgaaacaat
      781 gtacaaatat gctgtagaaa gtaaaatctt ttcttctgaa agcaaattcg tagtatttaa
      841 ctgtgctgaa tatgcagaaa atgctaactt attattaagt aacttatttg gttatgtcaa
      901 aggttctttt actggtgcta ataaggataa attcggcata gttgcacagg ctgatggagg
      961 aatattattc ttagatgaaa tacacagatt accaccagaa ggtcaagaga tgctttttct
     1021 tttgatggac aaaggcatat acagacgatt aggagaatct gacattgtac acaaagctaa
     1081 tgtctttatt gtgtgtgcca caacagaaga tatacattct tctctccttg ggacttttct
     1141 aagaagaatc cctgttacaa ttaacctacc cgctttacag gataggtcct taaaagaaag
     1201 attatctctt attaaatact tctttattat agagtcaaaa tataccaacg caactataag
     1261 agttcacaaa gatgtaatta aatcattact cctttacgat tgttttggta atataggtca
     1321 gcttaaatct gacatacagc ttatgagtgc aagagccttt ttaaattaca agactggact
     1381 taaggataat atagaaatag atttatccct tgttcctgat tacatctata ttggtctttt
     1441 aaaattgaat gaaaatcgta gtaatttaaa taaattatca gaaattgatt atatgctttt
     1501 ttatgaattt aatggtaaca acttagaagt tgaatataca aaagaactat ctaaatacaa
     1561 tatagataca tcaaacaatg gatttaaata ctctacagag attccttatc tggaaaatga
     1621 tataaggagc atgtttataa attacatgga aaaatactct acaaaattac tttcgatatc
     1681 ttcagtaact cattctgatt atacaagtaa tgaaattttt aaagtaatca atccaaagat
     1741 ttactatgct gttgaacatt ctttaaaaga ggctgaaaaa atatcgagaa acaaatatac
     1801 aaaacaaact tatattgctc tttcaatgca tataagtgca cttatagaaa atataaatca
     1861 agacacatat aatcctaagt acaatttata taatgataaa attttcttag ataaagattt
     1921 aaatatagct aaaatagttt taagtataat tgaaaacgac ttacaaacat ctttacctaa
     1981 agatgaagta aaatttatat ccatgtttct aaatagcacg tatcttgaac caaacaatca
     2041 atctgaaagt ataggggtca taatactcgc tcatggaaat tcaacagcaa gcagtatgtg
     2101 tgatgtagca aattcattgc ttggtacaaa ccattgtaat gcaattgaca tgcctcttga
     2161 tgtaaaagta agtactatac ttgataaagc tatagaattg gtaaaagaat gtgacgaagg
     2221 taagggtgta cttcttttaa cagatatggg ctctcttata gcttttggag aacttatttc
     2281 agaaaaaaca aaaatcaaaa ctcgctcaat tgaaatggta tctactccaa tagttttaga
     2341 agcagtaaga aaaagtacac taccagaaat gactttggat gaattagcta attccttaag
     2401 ctctttaaat ccatatattg gacgagcagc aattgattca acagaaataa gctatcctga
     2461 aaagtcaaga gagtatgtaa taataacgac ttgtataaca ggatatggtt cagctatgaa
     2521 agtagcagac tttataaatt cttcacttaa gaatattagt aaatacaata ttaatttagt
     2581 tcctcataat atagaaagtt ttaaaatgta taagaacaaa tttagtaata ataaaattct
     2641 ggcagttgtt ggaagtgtag attttaatgt agccaacacc ccttttatac acatagaaga
     2701 actactaaat ggatatggcc tagatatgct aaacaaaata atagagggta cattcaatgc
     2761 caatagtatt accaatataa aagaaacaaa tctatcatat aacagcttaa taataaactt
     2821 tatgacccaa aacttagagc ttttggactc tgttaaatta ttcaactatg tcaattcttc
     2881 cttacttaca ataatagaat taatagatga agaattaaag agcaaattta agctaggata
     2941 tatactacat tgctctttta tgatagaaag atgttttaaa aatacaactt ttccatataa
     3001 taacattact gaaataatta gtaaaaatca tgaactatat agtttaatac gtgaagcctt
     3061 taagccaaca gaagcttatt ttaatatttt tataccagac gaagaaattg cttatataat
     3121 agacatgatt aatgaatatc taaataaata aaacttatag cacttatttt gtgcatgtta
     3181 attaatatat gaaatggagg atgagatttt gaaaggaaaa ataaaacagt taacaatact
     3241 tacattaatt tttatattta taactcctgg atttgcattt gcagatactc caccagtacc
     3301 aaactcttca agagcagcac tacttataga ccaggaaact aaaagaatat tatttgaaaa
     3361 aaacattgat gagaaaatgc cattagcgag tttaagtaaa atgatgacct ttttattggc
     3421 aatagaagca attgataaga atcaagtaaa agagactgat atggtgaaaa tagacaagtc
     3481 tacagcttca gtaggaggtt ctacttgtaa acttaaagat ggtgatgaaa tatctctagg
     3541 agaacttatg cagggtctca tgctagtatc tggaaatgat gctgctctag ccatagctaa
     3601 gcatattggt aaaactgaaa agaattttgt aaatatgatg aacaagaaag cagaagaaat
     3661 aggaatgatt gatacgtatt attttaatcc aaatgggctt cctatatata ctgacccaga
     3721 gcataaagag cctcctattg agaatatatc aacagctcac gatatagtta cacttggaaa
     3781 gtatatgtat gaccattatg aaaaccaagt tacaagaata accactatgc aagtatataa
     3841 tgatactaaa aaagatttta cacattataa cacaaatcct ctacttgttt cagtacctgg
     3901 tgttgatgga ataaagacag gctacacaga taatgctgga tattgtcttg cattttctat
     3961 gatgatacct aaggatgcta aaaacgaaag aaatcataga ttaataggtg ttgtacttgg
     4021 tgatggtaac aagaaaaata gaatttcttc ttctgctagt ctattgaagt atggtaaaga
     4081 taatttccat tctaaaaaga tagcacataa gggggatatt atagagactc cttgtgttga
     4141 tggtattgat gattttaaga taaaagttaa ggttgataaa gatttgtatg gtgttgtttc
     4201 agataacgag aatatcaatc cgaaggtagt atttaaaaat atgaattatc ctatccataa
     4261 aggagatatt gttggtgttg caaagtatta taatgattct ggtaaatttg ttggaagtgt
     4321 caatgtaaaa agtgaaagta atataggatg tattcctttg aaagataaga ttaagattag
     4381 ggttgctaag ataaatagaa agcttgatgt taaaaattct gtttgtttta aagtatagta
     4441 aggcatacta ttaaaaaagt gtgttaaatt tataagactc cactttgtgg agtttttttg
     4501 ttttttacaa aaaattaaaa tagtggcaac aattgaggat gtgagggaga gttgccacta
     4561 ttttgtatag ggtattatat tatgttttta agctagtttt actatcttct atgtttatat
     4621 aataactctt gtgtgtgaat ttaatagata attcatatga aaattatgtg aagttttggg
     4681 tttttaaata aatttttatt taaataatac acataaatat ttcaatactc taagttttaa
     4741 aatactatag agtagcagat tttatttaaa aaatttgcta ctcttggata ttaaaaagct
     4801 gtaggaaaat tattttaaaa atcctacagc taatattagt atatacaaga agatttactt
     4861 tttttgaaac tagtttttaa ttatgcttaa ttatttatct caagatgtta aataaacttg
     4921 atagtccaga agatattttt cctattgttg atattggagc tgccacatgt tttttaactt
     4981 caaatgttgc atttgctact gaatctgtag cagttgtttc agatactgct cctgctttaa
     5041 ctaacacact tacattat
//
LOCUS       DAMJVM010000151         4685 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.156, whole genome shotgun sequence.
ACCESSION   DAMJVM010000151 DAMJVM010000000
VERSION     DAMJVM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.156"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            187..1110
                     /locus_tag="PC523_003341"
     CDS             187..1110
                     /locus_tag="PC523_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HDF2476824.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKTKIY
                     SNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTDLKKMKDICFSIEGNNEQGDLVIQNFIDSLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAITPLVEKNIENILDNGFTK
                     ALTGPVSRGDITPIMKHLSVLDKKDEEIYKILSLNLLKITALGNDNLLKCTKNITIEN
                     AVENLISKSEKYKKIYKLLGGKNDEKYYTDF"
     gene            1085..1912
                     /gene="panB"
                     /locus_tag="PC523_003342"
     CDS             1085..1912
                     /gene="panB"
                     /locus_tag="PC523_003342"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDF2476825.1"
                     /translation="MKNTILTFKNAKYEGKKLSMLTAYDYSMAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDTVKNAGR
                     LVKEGGAHAVKLEGGSNIIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKIQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIMRDSIKNYILEVKTGAFPQEKHSFSINESELEKLY"
     gene            1931..2779
                     /locus_tag="PC523_003343"
     CDS             1931..2779
                     /locus_tag="PC523_003343"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDF2476826.1"
                     /translation="MLVKEIKLLRNIIKDWRKDGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIERDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPIHFGGVCLVVSKLLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNRSLSLAKEELVKGNLN
                     PKNIKELITAKINSEHLAKIDYVEIVDSETLQPVGQIENSVLVAIAVFIGKTRLIDNF
                     TFELNI"
     gene            3142..3324
                     /locus_tag="PC523_003344"
     CDS             3142..3324
                     /locus_tag="PC523_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476827.1"
                     /translation="MFKDKIDECIHIMTAYIASLKEYYSFIETQIGDFIKKYGEDTVE
                     LCLHRIMILLCECGLA"
     gene            3629..4543
                     /locus_tag="PC523_003345"
     CDS             3629..4543
                     /locus_tag="PC523_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HDF2476828.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTSKDYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSAAEQKTEEFTGIEINKNITT
                     RELNLQSGVDPEVGQDLVNYVAQLNSDAIRLLDGLINLKERVLDGVLSCTLFTSNYPL
                     LLEHIIHEANLYRSYIVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFALKFNKLIEKANEMTDSNIKSITEETLNETVEFKDFKEEGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
BASE COUNT         1866 a          543 c          723 g         1553 t
ORIGIN      
        1 tttatagaat taattgtcaa ttttttatga aaatggtaat atatttatgg atggtatctt
       61 gttataagaa ctatacttta aatgttttta attattttgt cttatgtaac tcatatcctc
      121 aggaatgaga ttagaaatta agatgttgta tcacaaagtg gaggtgatac tattaaaata
      181 ggttttattg gttctggaaa agttggaaca tctttagcta attatttttt gtcaaaaggt
      241 tattgtgtat caggctttta tggaagaaat caattatctt tactagaatc aacaaattta
      301 acaaaaacaa agatttacag caatttgaaa gatataattt atgaaaacga tatattattt
      361 attacaacat cagatgattc tattgaaatt atagacaaaa aactttccaa atttaattta
      421 aaacataaat atatttgtca cacaagtggc tctttaaaat ctagtatatt gttctgttca
      481 aaaaaagcag gtgcattaat ttattctatc catcctatat ttgcattttc caataaaaat
      541 actgacttaa aaaaaatgaa agacatttgt ttttccatag aaggtaataa tgaacaaggt
      601 gatttagtta ttcaaaattt catagatagt ttgggaaacc aattttttat aagagataag
      661 gatacttcat ctacttatca tttagctaat gttttagttt caaatttagt attatcttta
      721 ctagatatag gagtaagtta ctttatcaaa ttaggtttat ctgaagagga atctttaaaa
      781 gccatcactc ctttagttga aaaaaatata gaaaatatac ttgataatgg ctttactaaa
      841 gctcttactg gtcctgtttc tcgtggagat ataacaccaa taatgaaaca tctatctgta
      901 ttggataaaa aagatgagga aatttataaa atactctcct taaatctact aaaaataact
      961 gccttaggaa atgataactt attaaaatgt acaaaaaata ttactataga aaatgcagta
     1021 gaaaatttaa taagtaaatc tgaaaaatac aagaagattt acaaactttt aggaggaaaa
     1081 aatgatgaaa aatactatac tgacttttaa gaatgctaaa tatgaaggaa aaaaactatc
     1141 tatgcttact gcttatgatt attcaatggc aaaaataatg gatgaatgtg atataaatgg
     1201 cattttaata ggagattcgt tgggaatggt aataaagggt gaagaaaaca ctctatctgt
     1261 tactatagat gaaattatat atcatacaaa ggctgtaaaa aatggcgtaa aaaatgcctt
     1321 aatcgtaagt gatatgcctt ttttatctta ccatgtttct atagaagata cagttaaaaa
     1381 tgctggtaga cttgtaaaag aaggaggagc tcatgctgta aagttagaag gtggctcaaa
     1441 cattataaag caaatagaaa gcattgtaaa tgcccagata cctgtaatgg gacatttagg
     1501 attaactcct caatcagtaa attcatttgg tggatttaag attcaaggaa atactagtga
     1561 aacagcaaga caacttattg aggatgctaa attaatagaa aaagcaggtg cattctctat
     1621 tgtccttgaa ggagtgccta cgaaaatagc tgagatggta actaactcta tttcaattcc
     1681 tacaataggt ataggagcag gaattaactg tgatggacaa attttagttt atcaagatat
     1741 gttgggaatg tttggagatt ttgttcctaa gtttgtaaag cagtatgcaa atataggaga
     1801 tataatgagg gattcaatta aaaattacat actagaagtt aagacaggtg cattcccaca
     1861 agaaaaacac agcttttcaa taaatgaatc tgaattagaa aaattatact aatattaagg
     1921 atggtacaaa atgcttgtaa aagaaataaa attactaaga aatataatca aagactggag
     1981 aaaagatggc tattctatag ggcttgttac aacaatggga tttttacatg aaggacacca
     2041 aagtcttata aaaaaagctg taaaagaaaa tgataaagta gtagtgagcg tatttgtaaa
     2101 tccaactcaa tttggaccaa atgaagattt taatagctat ccaagagata tagagagaga
     2161 ttttaaatac tgtatggata gcggtgcaac agtagtcttt aacccatcac cagaagaaat
     2221 gtatttaaaa ggtaattgta ctaccatcaa tgtttctgga cttacagatt ttctttgtgg
     2281 agctaaaaga cctattcatt ttggaggtgt ctgcctagtt gtttcaaagc ttctaaatat
     2341 agtaacacct gataaagctt attttggaga gaaggatgcc caacaattgg cagtaattaa
     2401 aaggatggtc aaggatttaa atatagatac agaaataata ggttgcccta taattcgtga
     2461 aaatgatgga ttagcaaaaa gttcaagaaa tacatatctc tctgaggaag agcgaaaatc
     2521 tgctctaatc ttaaatagga gcttaagttt agcaaaagaa gagctagtaa aaggtaatct
     2581 aaatccaaag aatataaaag aattaataac agctaaaata aattctgagc acttagcaaa
     2641 aatagactat gttgaaattg tagatagtga gacattacaa cctgttggac aaatagaaaa
     2701 ttctgtactt gtagcaattg ctgtgtttat aggaaaaacc agactaattg ataattttac
     2761 atttgagtta aatatatagt ctgcaatttt atataataca tctaaaaaat aataaattgc
     2821 ttagaaaata atagattaca ataactaaaa ttttaaaact aaattcctta aaaatatgat
     2881 taattgtaat tatatatgta tactattatt acacttatat taatctatat taaacctcta
     2941 ctaatttaag taaaccttta actggataat ttttccttta aaaatgaaaa tttatccagt
     3001 tttgcgatta taataaccag ttttacatat ccacttgtaa aatatatcta agcaatatat
     3061 catactatat agatttgtga gtgaaataat atctgctatt tactgaatat ttttattttt
     3121 cgttttttag ggggattagt tgtgtttaaa gataagattg atgaatgtat tcatataatg
     3181 actgcatata ttgctagttt aaaagaatac tattcattta tagaaactca aattggtgat
     3241 tttattaaaa aatatggaga agatactgta gagttatgtc tccatagaat aatgattcta
     3301 ctatgtgaat gtggtttagc ataaaattaa atacattaaa gtgggtaaga agagtcctat
     3361 gtaaaactcc tcttacccac tttaatatgt agacttagtt taatctaaat aactaagtta
     3421 taaaatcata ttataattta taataagaag caggttaata aatcaaattt gttttataat
     3481 ccaacataaa tattaagaaa aaggatgtaa taagattctt gttgatatta gtgtcaattg
     3541 aatttagtta atttatttat aatatacata aatttaaata ttacgaatag agtattataa
     3601 ataggatact ttctaaaggg aggaaattat gatagataat caaaaatatg ttattttatc
     3661 actagaatta cacttatttt tttcaagaat tatgaaagag catgctcttt ttttagaagc
     3721 aggatttaca agtaaagatt ataatcttgc tatggaagct gaccactata aaaagcaatt
     3781 tgaagattta ttatcataca cggttagtgc tagtaatggt ataattagac ctgatatatt
     3841 atattcagaa gaacttgtaa ctactcttac atcagctgca gaacaaaaaa cagaggagtt
     3901 tacaggaata gagataaaca aaaacataac tacaagagaa ttaaatctac aaagtggtgt
     3961 agacccagaa gttggtcaag acttagttaa ctatgtagct cagcttaact ctgatgcaat
     4021 aagattactt gacggactta ttaatttaaa ggaaagagtc ttagatggag tactatcatg
     4081 tactctattt acctcaaact atcctctact tcttgaacat ataatacatg aagcaaattt
     4141 atatcgttct tatatagttg acctcgaaaa taaaatagac attgagtcaa aaaacgctaa
     4201 agaaatagaa ttattctggg accatattat gatggaacac gctctattta tgagaggatt
     4261 attagaccct tctgaaggtg aactaataaa tacttcaaat gattttgctt taaaatttaa
     4321 taaattaatt gaaaaagcaa atgaaatgac tgattctaat attaagagta ttacagaaga
     4381 aactctaaat gaaactgttg aatttaaaga ttttaaagaa gaaggagcat caggaataga
     4441 acagtgtaag ataaaatcta taatattacc acttttagca gaccatgttt taagagaggc
     4501 taatcattat attagaatat tggagagtta taaaaacatg taattaaaaa atacttttaa
     4561 aacaatattt agataaaata aaggtacttc aactgtacct ttattttttt atacgcttat
     4621 agaatttaaa caagttattt ataaaacaaa atcataatca tctataatcc acaaatttat
     4681 tttat
//
LOCUS       DAMJVM010000152         4588 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.157, whole genome shotgun sequence.
ACCESSION   DAMJVM010000152 DAMJVM010000000
VERSION     DAMJVM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.157"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            156..803
                     /locus_tag="PC523_003346"
     CDS             156..803
                     /locus_tag="PC523_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2476829.1"
                     /translation="MNILIADDEISMLKILCAYFKKENFNVLTAKNGEEALDIFYKNK
                     IDLAILDWMMPIIDGIEVCKEIKENSNTKVLMLTAKSQSEDEIEALNIGADEYIKKPF
                     DPRVLIIRAKKLVNYKDTINIKDLKIDFESSKVFKGDNELLLTKKELELIRCLINNKG
                     MILSREKLLDLVWGLDYEGDFRTVDTHIRRLRVKIGEDIIKTYRGLGYSLEDFND"
     gene            796..2190
                     /locus_tag="PC523_003347"
     CDS             796..2190
                     /locus_tag="PC523_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2476830.1"
                     /translation="MTKLNKKLSISISIVVVVVYIVSITINSMFIHRYYLHEKRNILD
                     NIENEIKDKDIDKLKSDIDSIEEKNNADIVYIELDKNHKDKGNIDRINQDLLNAFWKK
                     GLSLNKFWIEENSLKNIGKKSINKIYNQGKTKYSLLVKFIKKDNYLFAISIPIEHSEE
                     TIGIVNRFNIIMGVFSVVIITILTFILSNRVIKPIEKLKFLSKDISELNFRIEDIKTN
                     DEIEELAYSINMMSEKLEKAHNELNKRNENLKTFISDASHEIKTPIALIKAYAIGMKD
                     GLDDGTYTDTIIEQAENMTNTINSLLYWAKYEKKERSLCLVDLKERLYKSLKNYELLI
                     DKGGISVKCNVDDRELIISSDEDSIEVVFNNLISNAIKYTNNNEIEINLFEENERIIL
                     SIKNGIDCDMEDEIENIWKPFYVLEKSRSKELSGTGLGLTIVRTILEENNFRYRVEIC
                     DKIIAFYIIFIKLI"
     gene            2614..>4588
                     /locus_tag="PC523_003348"
     CDS             2614..>4588
                     /locus_tag="PC523_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein CwpV"
                     /protein_id="HDF2476831.1"
                     /translation="MKIPKKIAAMLTVTMIAGSSTAGIASAQTVATNLTGQERYETAV
                     KISQDGWKNADEVVIVNDSSIADALSATPFAKAKNAPILLTGKDKLNDKTKAEIQRLK
                     AKKVYLIGGTSVLSTNIEKEIKDLKISFERISGAERYQTSLELAKKLDAISDVKKIAV
                     VNGEKGLADAVSVGAPAAQNNMPVILADSKNGTAVADKFIKDAGITQSYVVGGESSIS
                     EAVKNKLPNSTRLGGTDRNDTNAKVIKEFYKKTDLKNAYVTKDGMNKQDQLIDALAVG
                     VLGAKNQSPVVLVGKNLSASQKSLVNSKSFDKITKVGGNGNETAFNEMKSLQEVKTVE
                     AKTISELKSAIDKATANDVINFKPTSEVKEAFTIQTDKAVTVNLNGTYTKTVTINMPN
                     GDVNNYAKVDDVVIDDVKDGTFVNYGKITNLKVNDKNGAKIENNSKGEIGSLTVASGA
                     SQVKVTNGGKITTVTNNSKGTTIDNKGTISSVKGDNSPTISGNSPSSNSSGGSSSSGG
                     SSHGGGSGGSSSSKVGQIKFNATDKQIKMASGDKTVDPSDNTYVLKLTNGTVKDGDVK
                     ANVEVTGLPSGLDYTAVGNKSANTITITVSGTASQALQADLDNVSVLVKAGAVSETTA
                     TDSIANATFEVKKYVAAPAVGQLTFDATDKQIKM"
BASE COUNT         1979 a          451 c          816 g         1342 t
ORIGIN      
        1 catattaaaa tatataatca agatattgtc gaggtattat aatatataac taatgtatta
       61 taaatagttg taaacttcct gtaacatata ttttatataa ttaaagtaat ggatataata
      121 ttaaatataa taatgaattt tagggatgtg aataaatgaa catattaatt gctgatgatg
      181 aaattagtat gcttaagata ctttgtgcat attttaaaaa ggaaaatttc aatgtactta
      241 ctgcaaaaaa tggggaagag gctttagata ttttttataa aaataagata gacttagcaa
      301 tattagattg gatgatgcca ataattgatg gaatagaggt ttgtaaagag ataaaggaaa
      361 atagtaatac aaaggtactt atgttgactg ccaaaagtca aagtgaagat gaaatagaag
      421 cactcaatat tggtgcagat gaatatatta aaaaaccatt tgaccctagg gttttaataa
      481 taagagctaa gaaactagta aactataaag atacaattaa tattaaagat ttaaaaatag
      541 attttgagtc aagtaaagtt tttaaaggtg ataatgaact attattgact aagaaagaat
      601 tagaattaat tcgttgttta ataaataata aggggatgat tttatctaga gaaaaacttt
      661 tagatttagt atggggttta gattatgagg gtgattttag aacagtagat actcatataa
      721 gaagattaag agtaaaaata ggagaggata taataaaaac ttatagagga ttagggtata
      781 gtctggagga ttttaatgac taaactgaat aaaaaattgt ccataagtat atcgatagta
      841 gtcgtagttg tttatatagt gtctataact ataaatagta tgtttattca tagatattat
      901 ttacatgaga aaagaaatat attggataat attgaaaatg aaattaaaga taaagatata
      961 gataaattaa aatcagacat tgattcaatt gaagaaaaaa ataatgcaga tattgtatat
     1021 atagaattag ataaaaatca taaagataaa ggtaatatag atagaataaa tcaagattta
     1081 ttaaatgcat tttggaagaa ggggttaagt ttaaataagt tttggattga agaaaatagt
     1141 ttaaaaaata ttgggaagaa aagtataaat aagatataca atcaaggtaa aacaaaatat
     1201 agtttacttg taaaatttat taaaaaggac aattatttgt ttgctatatc tatacccata
     1261 gaacattctg aagaaactat tggcattgta aatagattta atataataat gggagttttt
     1321 tcagtagtta taataactat acttacgttt atattatcaa acagagtcat aaaacctata
     1381 gaaaagttaa agtttttatc taaagatata tctgaattaa attttaggat tgaagatata
     1441 aaaacgaatg atgaaataga ggagttagct tacagcataa acatgatgag cgaaaagctt
     1501 gaaaaagcac ataatgaatt gaataaaaga aatgagaatc taaaaacttt tatatcagat
     1561 gcatctcatg aaataaaaac tccaatagca cttataaaag catatgcaat aggtatgaaa
     1621 gatggcttgg atgatgggac ttatacagat acaattattg aacaagcaga gaatatgacc
     1681 aatacaataa attctctttt atattgggca aaatatgaga agaaggagag aagtctgtgt
     1741 ctggtagatt taaaggaaag attatataaa agcttaaaaa actatgagct gttgatagat
     1801 aaaggtggga taagtgttaa gtgcaatgta gatgatagag aacttataat aagttcggat
     1861 gaagatagta tagaggtggt atttaataat ttaattagta atgctataaa gtatactaat
     1921 aataatgaaa tagagattaa tctttttgaa gagaatgaga gaattatatt atccataaaa
     1981 aatggtatag attgcgatat ggaagatgaa atagagaata tatggaagcc tttttatgtt
     2041 ttagagaagt caagaagtaa ggagctttct ggaactggtc ttggtcttac tatagttaga
     2101 actatacttg aagaaaataa ttttagatac agggttgaaa tttgtgataa aattatagca
     2161 ttttatatta tatttataaa attaatttga attaaaatgc aaatataaag aataattaaa
     2221 aaaatatagc aattatttgt ttttcatatg gataaaattg gttatttaat atataattac
     2281 aagtataaat aataaaattt atttgtaaaa agagtagcac ttttaattct aaaggctact
     2341 tttttatgtg aaaccctact ttttacaaca attcattaac ataaaaatca aacttacctt
     2401 aaaatataaa tttacaaatt tttcaatcaa taaaaaaatt tctttattcc ttatataatg
     2461 actgcaatgt atcaaaataa ttacaaatta attatgaaat cattatagtt agaaacctct
     2521 ttaaaattag atattaagta tcctttagaa ttagaacggg aacaaagtta aaaaattaag
     2581 taatatcaaa tggacaaggg ggaagaaaat acaatgaaaa taccaaagaa gattgctgca
     2641 atgcttacag taacaatgat agctggaagt tcaacagcag gcatagctag tgcacaaact
     2701 gtggcaacaa atttaacagg gcaagaaaga tatgaaactg ctgttaaaat aagtcaagat
     2761 ggatggaaaa atgctgatga ggtagtaata gtaaacgact catctatagc agatgctcta
     2821 tctgcaacac catttgcaaa ggcaaaaaat gctccaatac ttttaacagg taaagataaa
     2881 ttaaatgata agacgaaagc agaaatacaa agattaaaag ctaaaaaagt atacttaata
     2941 ggtggaactt ctgtattaag cacaaacata gaaaaagaaa taaaagattt aaaaatatct
     3001 tttgaaagaa tctctggagc tgagagatat caaacatcct tagaattggc aaagaaatta
     3061 gatgctataa gtgatgttaa gaaaatagct gtagttaatg gtgaaaaagg acttgcagat
     3121 gctgtaagtg taggagcacc agctgcacaa aacaatatgc cagtaatact tgctgattct
     3181 aaaaatggaa cagcagttgc agacaaattt ataaaagatg ctggaataac tcaatcttat
     3241 gtagtaggtg gagagagttc aatttctgaa gctgttaaaa ataaactacc aaatagtaca
     3301 agactaggcg gaacagatag aaatgataca aatgcaaaag taataaaaga attttataaa
     3361 aagacagatt taaaaaatgc ttatgtaact aaagatggaa tgaataaaca agaccaactt
     3421 atagatgcac tagcagtagg tgtattaggt gctaaaaatc aatcaccagt agttttagtt
     3481 ggaaagaact tatcagcttc acaaaaatct ctagttaact ctaagagttt tgataagata
     3541 actaaagttg gtggaaatgg taatgagaca gcatttaatg aaatgaaatc actacaagaa
     3601 gtaaaaacag ttgaagctaa aacaatttct gaattaaaat cagctataga taaagctact
     3661 gctaatgatg ttataaactt taagccaact agtgaggtta aagaagcatt tacaatacaa
     3721 actgataaag cagtaacagt aaacttaaat ggtacatata caaagactgt gactatcaac
     3781 atgcctaatg gtgatgtaaa taactatgca aaagtggatg atgtagttat agatgatgta
     3841 aaagatggaa cttttgttaa ctatggaaag ataaccaact taaaggttaa tgataaaaat
     3901 ggagctaaga tagagaataa ttctaaaggt gagattggta gtttaacagt agcatctgga
     3961 gcaagtcaag taaaagtgac taatggtgga aagataacta cagttacaaa taattctaaa
     4021 gggactacta tagataataa aggtacaata agtagtgtta agggagataa ttctccaaca
     4081 ataagtggaa atagtccaag ttctaactct tcaggaggaa gttcatcaag tggaggttct
     4141 tcacatggag gaggtagtgg aggaagttct tcatcaaagg taggtcaaat aaaatttaat
     4201 gcaacagata aacaaataaa aatggcatca ggtgataaga cagtagatcc atcagataac
     4261 acatatgtac taaaattaac aaatgggaca gtaaaagatg gagatgtaaa agctaatgtt
     4321 gaagtaacag gattaccatc aggattggat tatacagcag taggaaataa atcagcaaac
     4381 acaataacaa taacagtatc aggaacagca agccaagcat tacaagctga tttagataat
     4441 gtaagtgtgt tagttaaagc aggagcagta tctgaaacaa ctgctacaga ttcaattgca
     4501 aatgcaacat ttgaagtaaa aaaatatgtg gcagcaccag cagtaggtca attaactttt
     4561 gatgcaacag ataaacaaat aaaaatgg
//
LOCUS       DAMJVM010000153         4578 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.158, whole genome shotgun sequence.
ACCESSION   DAMJVM010000153 DAMJVM010000000
VERSION     DAMJVM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.158"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            129..527
                     /locus_tag="PC523_003349"
     CDS             129..527
                     /locus_tag="PC523_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2476832.1"
                     /translation="MTKKIKHVLITTNNIENYINFYKKFGFSFKETSEKYKFNAGNVK
                     INLNIKGHEFLPQGENVQTISTNLCFKIDTNIDEPKKYLEQNEIQIKRVIVECTEVFS
                     NMKSICFKKTDVNLIELSNHRILESKPWKI"
     gene            515..1453
                     /locus_tag="PC523_003350"
     CDS             515..1453
                     /locus_tag="PC523_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDF2476833.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDVHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKNNKTRGALIQ
                     GIFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVIILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKPIIFGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMSQQ
                     MDSDSELAGQIVVFTSAFSIITVFLIIFILKQNNLI"
     gene            complement(1813..2037)
                     /gene="secG"
                     /locus_tag="PC523_003351"
     CDS             complement(1813..2037)
                     /gene="secG"
                     /locus_tag="PC523_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDF2476834.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(2180..2401)
                     /locus_tag="PC523_003352"
     CDS             complement(2180..2401)
                     /locus_tag="PC523_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2476835.1"
                     /translation="MVFYEFRYFRPAFWGTPVAKKGLQFCIDGLRNLGYPYITIWVLK
                     DNIRVIKFYKKMVLLVMILRKIYVLEKNY"
     gene            2467..2897
                     /locus_tag="PC523_003353"
                     /pseudo
     CDS             2467..2897
                     /locus_tag="PC523_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429569.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            complement(2998..4290)
                     /gene="eno"
                     /locus_tag="PC523_003354"
     CDS             complement(2998..4290)
                     /gene="eno"
                     /locus_tag="PC523_003354"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDF2476836.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT         1596 a          723 c          536 g         1723 t
ORIGIN      
        1 gcaataactt atataaaatt tatcaatcga cataaaaatt aataattttt atagacatta
       61 tcaatctata taaaatttaa taacccatat aaatttaaat aatttttata aaatcttaag
      121 gagttgttat gacaaaaaaa attaaacatg tactcattac tactaataat atagaaaatt
      181 acataaattt ttataaaaaa tttggttttt catttaagga aacaagtgaa aaatataaat
      241 ttaatgctgg aaatgttaag ataaatctaa acataaaagg tcatgaattt ttaccacaag
      301 gtgaaaatgt acaaacaatc agtactaatt tatgctttaa aatagataca aatattgatg
      361 agccaaaaaa atacttagaa caaaatgaga ttcaaattaa gcgagtaatt gtagagtgta
      421 cagaggtctt tagtaatatg aaatctattt gttttaaaaa aactgacgta aatttaattg
      481 aattatcgaa tcatagaatc ctggagagta aaccttggaa aatttaatcc tatcaataaa
      541 tgttgttctt cctttgtttc tcacaatgtc acttggatat gttctaaaaa aattaggtct
      601 atttgatgtc catactttaa ataaaatgaa taatgtctgt ttcaagtctt tcttgccatt
      661 attattattt tttaatatat acaatacaga tttagatggt tctcttaatt ttaaacttat
      721 ggctgtagct gttataatta ttttttcaat cttcttaatc ttagtcttta ttattcctaa
      781 aattgaaaaa aataataaga caaggggtgc attaatacaa ggcatatttc gtagtaattt
      841 cgttatattt ggtattcctg tgacaatttc actatttggt gataagagta ttggtgtcac
      901 atctttattg attgcagtta ttgtacctat gtttaatgta ttatctgtta taatacttga
      961 aatttttaga ggaggcaaag taaatataaa acatatgatt aaaagtgttt taacaaatcc
     1021 actcataatc gcctctttta taggattgac aatgctcatt ttaaatatta atatacctac
     1081 atttttacac aaaactattg gtgatatttc taagatagct actcctttat cgcttataat
     1141 tctaggaggt tcttttagtt tttctgctat aaaaggacat ataaaaccta ttatatttgg
     1201 ggtatgtgga aaacttatac ttgtacctgc tatatttttg ccaattagta tttttcttgg
     1261 atttagaaat gtagaattag cttcacttat gataatgtta tctgctccaa ctgcagtttc
     1321 ttcatttaca atgtcacaac agatggattc tgatagtgaa ttagctggac aaattgtagt
     1381 atttacttct gcattttcta taattacagt atttttaata atatttattt taaaacagaa
     1441 taatcttatt taatgatatg caataggata aattagtatt atgagaaaca tatataaaat
     1501 tgatttagtt acataaaaac atatgattat actattttta ctatataact ttatgtatat
     1561 tatcttgtga aacatgttga aagtactgtt gttatttatt gcttgattta aaaaatatat
     1621 aaatacaaac aatagatatt aatagatagt caatctataa aataaaaact ggactaatca
     1681 tataaaatca ttagtccagc ttttatttta cgtatttttt atgttgactt acttttatta
     1741 aaaactaaat ttaattttta aatgtctagc aaatgcacta attaaataat ggccagttat
     1801 tcaacaatat cactacttct taactataag taatgccaat gcatttataa aaaataatac
     1861 tgctgttatc tttgtaactc tagcaagaaa tgcttgtttc ccttttggtt taaagtaagc
     1921 ttgacttccc tctccaccaa actctgaagg taaagcattt tttgaatctt gaggcattat
     1981 gctaattact agtaaaatcc ctagcactac ttgcacaccc attaaaatgt tgctcattct
     2041 tgcccctcct atctgtttat tacttaaatt tcattactct ttagtgtatc acaataatat
     2101 agccatttca agactattat gctataaatc acatacaaaa aaatatataa ttactgtgtc
     2161 ttttttaggt aacatatttc taataatttt tttccaatac atatatcttt ctcaaaatca
     2221 tcacaagtaa aaccattttt ttatagaatt ttataactct aatattatct tttagaaccc
     2281 atattgttat atatggatat cctaaatttc ttaatccatc aatacaaaat tgtaatcctt
     2341 tttttgcaac aggcgttccc caaaacgctg gacggaaata tctaaattca taaaaaacca
     2401 tcctatttac aaattaatac gactatcata ttatattagt acgataatcg tattatatgg
     2461 aggtaaatgt caatgaatgc tataaaagaa ctaagcaacc tatggcacaa cgttggtata
     2521 caaatgaaaa cagaaatgtg tagtggtaaa tatcctacta tttctggtat ttctttagta
     2581 gaattaagta ttttagaaat agtcgagcaa catccaaata gtatgataaa agaaattagt
     2641 tcaactttag aatttccaaa aagcacatta acaagtgatg taaaacgtct tgagctaaaa
     2701 aacttataaa tagaaatcct tgctttaagg ataaaagggc gtataattta gatttaactg
     2761 aattaggttt aaaagctcaa aaagaacata gagaaataga agcaactgta tttaaaaata
     2821 tcctacatca attagataaa ggtgagatag aaacatttat tcatattttt tctcaagctg
     2881 tgaataaaat taggtaataa tatataaaaa aggataagtg aattgaaaaa tcaactaaca
     2941 cttatcctta tataagacaa acttaaacgt tttatactat ttctttaatc ttaataacta
     3001 tttttttaag ttatagaaag attttaatcc acagtatcta gcttgctctc caaccatttc
     3061 ttctattctt aataattggt tgtacttagc aactctatca gttcttgatg gagcaccagt
     3121 ttttatttga ccagcattta ctgcaacagc taaatctgct atagtagaat cttcagtttc
     3181 tcctgatcta tgagatataa cagcagtata tcctgctctc ttagccattt ctatagcatc
     3241 taaagtttca gttattgtac ctatttggtt aactttaact aatatagagt tagctactcc
     3301 attttcaatt cctttttcta gtctctctgt gttagtaaca aataagtcgt caccaactaa
     3361 ttgtaattta tttcctaatt tttcagttaa taacttccat ccatcccaat cttcttcatc
     3421 taaaccatct tctatagtta ttattgggaa gttgtttgac caatcttcat ataaagcaac
     3481 catttcagca gcagttaact cttttccttc tcctgctaaa acgtattttt tagtttcttt
     3541 gttgtacatt tctgtagcag caacgtcaag acctaacata acatcttcac ctggcttata
     3601 cccagctttt gttatagctt caactattaa ttctaaagct tctctgtttg aacctaagtt
     3661 tggagcgaat ccaccttcgt cgcctacacc acaagctaat cctttttctc ctaaaacttt
     3721 ctttaatgaa tggaatactt ctgctcccat tcttaaacct tctttgaaac aacaagctcc
     3781 tactggtaat atcataaatt cttgaacatc aacgttgtta tcagcatgct ctccaccgtt
     3841 taatatgttc atcattggaa caggtaattg tttagcattt actccaccta aatattggaa
     3901 taatggtaaa cctatttcat cagcagctgc tctagctact gccattgaaa cacctaatat
     3961 agcattagct cctaatttac ctttgtttgg agttccatct aattctatca tgattgcatc
     4021 tatagctggt tggtcaaatg catccattcc ttctatttca ggtgctatta tttcatttac
     4081 gttagcaaca gctgtttcta caccttttcc taagtatctt cccttatctc catctcttaa
     4141 ttcaacagct tcaaaagctc ctgtagaagc tcctgatgga actattgctc ttcccattgc
     4201 tccatcttct aatactactt caacttctac agttggattt cctcttgagt ctaatacttc
     4261 tctagcatat actaattcaa taactgacat ttgtatatct cccttcgatt ttctattagt
     4321 taaaatttca tttactgtaa acatccgtct actattttaa tctactttct atattatagt
     4381 acataaagtt gcttttgaaa attaggtaca aatttatttg atgggtaaat ttgtaaaagt
     4441 tcctattttt attttatttt ttataatatt ttaatttata taatataaaa aaacttgtct
     4501 aaaatccaaa accaatctta aatacacgaa tattataact agatttaaaa cattaaacat
     4561 aaaatgtaca actaatga
//
LOCUS       DAMJVM010000154         4443 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.159, whole genome shotgun sequence.
ACCESSION   DAMJVM010000154 DAMJVM010000000
VERSION     DAMJVM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.159"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(<1..2558)
                     /gene="carB"
                     /locus_tag="PC523_003355"
     CDS             complement(<1..2558)
                     /gene="carB"
                     /locus_tag="PC523_003355"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HDF2476837.1"
                     /translation="MPKLDNIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIAENEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHQLKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFSDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGV"
     gene            complement(2751..3800)
                     /gene="carA"
                     /locus_tag="PC523_003356"
     CDS             complement(2751..3800)
                     /gene="carA"
                     /locus_tag="PC523_003356"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDF2476838.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESIGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITVFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(4057..>4443)
                     /locus_tag="PC523_003357"
     CDS             complement(4057..>4443)
                     /locus_tag="PC523_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDF2476839.1"
                     /translation="VVLATVNNHDKDEFIEIAKDMKELGYTFVATEGTAKSLRENGIE
                     ADIVNRVEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRTAIEFSTEIMTSL
                     DTLKALVEVKKKHLNKDKLKVYNIAE"
BASE COUNT         1271 a          885 c          451 g         1836 t
ORIGIN      
        1 actccactta ctttgcttat atatggaact gttctactag ctctaggatt aacttcaatt
       61 atatataatt cattttgaaa ttctataaac tgtatattaa ccataccaag cacattaagg
      121 tctaaagcta tttttttagt atattctact atcttttctt ttatttcatc agatatattt
      181 tgacttggat acattgttgt actatctcca gagtgaactc cagctctttc taaatgttcc
      241 attataccag gtattaatat atcttcctta tcacatatag cgtctacttc tatttctcta
      301 ccagttagat atttatctat aagtactgga ttcttatggt ctctatcaaa tgcatcatcc
      361 aaatactgaa ttagtttttc ttcattataa gttatttcca taccttggcc accaagtaca
      421 taagatggtc taactagtac tggataacct aattcctttg caatttcaac accttcatta
      481 gttgtccata caccttttcc ttttggtcta tttatatcta gtctttctag taaatcatca
      541 aacttctctc tatcttctgc tgcatctata tcgctaaaat ctgttcccag tataggtata
      601 tttttttcat gtaagaactt agccagtttt atagctgtct gtccaccaaa ctgtaatatt
      661 acaccttctg gtttttcttt ttctattata tttaaaacct cttcttctgt taatggttca
      721 aaatataact tatctgatgt atcaaaatct gtacttactg tttcagggtt attgtttaca
      781 attatggttt ctatgtccat ttttcttaaa gattttacac aatgaactga acaataatca
      841 aattctatac cttgacctat tcttattgga ccagaaccca atactaccac ttttctctta
      901 tcactaacaa ctacctcatc atattgctca taagttgaat aatagtaagg tgataaagct
      961 tcaaattctc ctccacacgt atcaaccatt ttatatactg gttgtatatt atataaactt
     1021 ctcaattcat ataatttttc tggacttatc ttcattaagt cagatatacc tttatccgaa
     1081 aatccttttt tctttaattg atgtaaatat tctttatcta agtcttctat ttttaaaact
     1141 tttaattttt cttcttgctc tactatccat ttgaatttat ttataaacca cttatcaaca
     1201 ccagttattt ctgatatcat ttctacttta tagcctcttc ttatcatttc agctaagtca
     1261 aatagtctct catcatcagg cactactact ctagccttta attcctctaa tgttctacct
     1321 tctgatggtg catgaaccaa cgaatatttt cctgtttcaa gtgatcttat ccctttaaga
     1381 atagcagctt caaagttact acctatactc ataatttcac cagtcgccat catcttagtt
     1441 cctaattttc tattggcatg tctaaattta tcaaatggcc actttggtat ttttaccact
     1501 acataatcta atgttggctc aaaacaagca taagttttct tagttactgc attttctatc
     1561 tcatctaaag tgtagcctaa agctatctta gctgctactt ttgctattgg atatcctgta
     1621 gcttttgatg ctaaggctga tgacctactt actcttgggt ttatttctat tatcgcatat
     1681 tctaaactat gtggatttaa agctatttga acgttacatc caccttgaac ctctattgca
     1741 tttattatgt ctattgatgc ctttctaagt agttgatact cttcatcact tagggtttga
     1801 cttggagcaa caactatact atctcctgta tgaactccga ctgggtctac attttccata
     1861 ttacatacag ttatacagtt tccatttcca tctctcatta cttcatattc tatttctttc
     1921 caacctttta tactcttttc aagaagaact tgtccaacag ggcttaattg tagtccatgt
     1981 gaaagtattt ctcttaattc ttcctcattt tcagctatac ctccaccagt tccacctagt
     2041 gtgtatgctg gtcttacaac tactggatat cctattttat tggcaaactc aagacctgct
     2101 tctaaatttg taactatgtc actaactatt acaggttgat ttatttcttt cattacttct
     2161 ctaaataaat ctctatcttc tccctttttt atagactcta tagatgtccc tataactttt
     2221 acattatatt tatctaatat cccagcatca tgtaattcta cagctaaatt taatcctgtc
     2281 tgaccaccca tacctgcaag taaactatct ggtctttctt tttctattat tttttctata
     2341 aattctattg ttaatggttc tatatatatt ttatctgcaa tctctttgtc agtcattata
     2401 gtagctgggt tactatttac taatacaact tcaattccct cttcttttaa tgcttggcag
     2461 gcctgtgttc ctgaatagtc aaactctgca gcttgaccta ttattattgg tcctgaccct
     2521 aatactaaag ttttctttat attatctaat ttaggcataa ctctttctcc ttatctaaac
     2581 aatttttaca gctcttatct tcatcatcta tacaatcaat tttaattact ttatttcata
     2641 ttaagttcaa atttacgtat taatacatcc atttaataca tctatgttaa ttaaaccaaa
     2701 ctttttgatt taagttattc ttatctttta gttatctatt ctaatattat ttattttact
     2761 aactctaaaa atttatcaaa aatatagtca ttatcttttg gtcctgggca agcttcagga
     2821 tggtattgaa cactgtatac atctaacttc tcatgtctca ttccttcaac agtattatca
     2881 tttaaattta tatgtgtcac tttcatttgt tcaggaactt ctgaaacata ataaccatga
     2941 ttttgagaag ttataaatac ttttccctct tctaaatctt taactggatg atttccacct
     3001 ctatgtccaa atttaagttt agcagttttt cctccaagag ctaaagctaa aagttggtgt
     3061 cctaagcata ttccaactat aggtttttta ccaataagag cctttatatt ttctataaca
     3121 tcttctaggt cttctgggtc tccaggtcca tttgacaaga atattaaatc agggtttata
     3181 ctcaatacat cttctggaga tgttgtagct ggaaatactg ttatatcaca tccacgagct
     3241 ataaacgatc ttaatatatt tctttttacc ccaaaatcca taacagccac ttttggacca
     3301 tttcctttta tatgctctac ttcttttctt gtaacagttc taactgcttc tgtatttgaa
     3361 aacttatcca atttatgttt aacatcttct aatttactgt cttctaatgt aattatacct
     3421 ttcattgtcc cattatttct aagtatctta gtcaaagctc ttgtgtctat tccttcaagt
     3481 ccaataacct tattttgctt taaatattgg tctatatcaa tttcacatct aaaattactt
     3541 ggagaatcac ttttctccct tactatgaat ccctttactt gaactttttc agactctgct
     3601 gatgataaat ttataccata atttcctaca agaggataag tcatagtaac tatttgacca
     3661 tagtaagaag ggtctgttaa aacttcacca tatcctatca ttgaagtatt aaatactact
     3721 tctcctatac tttcttctaa atatccaaat gcctttccta taaaaactgt tccatcttct
     3781 aatattaacc ttgctttcat actacagtac ctcctataat tctctttaga taaactaaag
     3841 aacattaact aaatataagc ttttaaatat ctatatctat ttttcacttt tacattctag
     3901 ttttatactt atttgcattt attttttatc tttttctatt atactacaaa tttcttaaaa
     3961 aaaagaagtt atctcaaaag taaaaacccc tagaaaaaat aacctctttt tttaatttaa
     4021 taattaaatt cttaaactat acaatcaact taatttctat tcagctatat tgtaaacttt
     4081 taacttatct ttatttaaat gtttcttctt aacttctact aaagctttta gtgtatccag
     4141 agatgtcatt atttcagtag aaaactcaat agctgttctt cttatcttaa acccatctct
     4201 tgtggagtca tttcctttag ttggtgtatt tattactata tcaacttgtt tatttcttat
     4261 agcatctaat atgtttggtc tagactcttc tactctattt actatatcag cctctattcc
     4321 attttctctt aagctcttag ctgttccttc tgtagcaaca aaagtatatc ctaattcttt
     4381 catatctttt gctatttcta taaactcatc tttgtcatga ttgtttacag tagcaagtac
     4441 tac
//
LOCUS       DAMJVM010000155         4439 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.160, whole genome shotgun sequence.
ACCESSION   DAMJVM010000155 DAMJVM010000000
VERSION     DAMJVM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.160"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(331..1734)
                     /locus_tag="PC523_003358"
     CDS             complement(331..1734)
                     /locus_tag="PC523_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HDF2476840.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILVNSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKANTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGV
                     TGEADAISASPVAARDGVPIILTDGNSMGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFDTNKRVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     DNGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
     gene            complement(2197..4092)
                     /locus_tag="PC523_003359"
     CDS             complement(2197..4092)
                     /locus_tag="PC523_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HDF2476841.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAQDSDNKLTATIKSTS
                     TVEVKRAKDSTNVITLNVGDQHLDFSKVITSEDGTFEGYEKSYSDIESKELHTITVKN
                     ADLQDITAEELFDGIRLTTLGRELVNKVKNGYTLTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKLETISVSSKNHKLVRELHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIESEILRV
                     LGSNLSSKKIYIVGGESKISKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
BASE COUNT         1287 a          792 c          449 g         1911 t
ORIGIN      
        1 aacttataaa agaaaggtgt ctgcttcatt caaaaaaaca aatttaatac tactcaaact
       61 attacaaatt ttaaattcaa tattattaaa acaatgaaaa taaaaataag aatgtatttt
      121 tagacatatt atttatatct aaatacattc ttattttcag ttcattcaat atatacttat
      181 ttttcaatta agctcctaat tttatctact atatatgaag ctatttctct acctatttta
      241 aacaatctaa atgaattttt attttttaat gatatttttg tgatttatct gacatattag
      301 tagtaattat aacttacaaa ttgtttttat ttattcttta tatcttccat aaaattcatg
      361 tgtagcatat agattcttat ctcctgttat agctatacca aatcccatta tttcataccc
      421 tttatgcaac atattagtac cttcttctgg atttgtcttc cactcattaa aaataaagtt
      481 tcctatatca tctgcatctc tatttgtata cacaggttta tctggaattt tatatttaat
      541 tatattttca ccttgaactg caatatatcc aggtttaatc tcactccaat ctaggtattt
      601 caaaaaagta gaatatgaat tttttccatt tatcgtatga gataaggtct tcttatttgc
      661 cattaaatta gaccaatcat ttgctaatcc ttctaatcta cttgctacag ataaggctct
      721 tactccatta tcttttctgt actgctttgt aagagcatat actttaccct ttattcgctc
      781 tttaaatgct ctatcatttg gattgtaata taagccttgt tgatttggtg aagcttgatt
      841 tatacattgc ttgataacat tctgatttat gcctcctagt acagtcaatt tgtctgcacc
      901 acttaaaata ctcttgttac tgccattttc aactaaaacc acaggagcat tttttgctat
      961 tgtagaagca gccaaagcgt ctgttaattg caacccttta cttaaataaa attctttact
     1021 atttttataa aattcttgta taaccctctt attagtatca aatctatctg ttccacctaa
     1081 tctaatagag tttgtactct taaccaattc gtcactgatt atttcactag aaccaagtgc
     1141 ataactcttt aaacctgttg tgtcaaatcc catactattc ccatctgtaa gtattatagg
     1201 tactccatct ctagctgcta ctggtgatgc acttatcgca tctgcttctc ctgtaactcc
     1261 atttgctata attactttat ctattggctt aatttctttg attttttcag ctatttttat
     1321 gcttgtatca aatctatttt caccaaatat tctttgtaca accttctttt tggatagtaa
     1381 ctgattctca acattcttac ttattgttga ttctgagcct attatatata cagtatttgc
     1441 tttgtttaat ctttccatag ttgtatttgg aatctgattt tgttttgtca ttaaaatagg
     1501 tgcatttaaa gctccagcca aaccacttgc acttaatcca tctgccaagc tgttatctga
     1561 attgactagt attgctgtat tatagttttg cttatctgct atttttgctg ctgtctcata
     1621 tctgtcattc ccttttattt gctcaactga tgtcaacgca tttaccatag gagtatttac
     1681 cattactaaa aatagtgtaa gtcctatagc caatatcttt ttactattct tcatattatt
     1741 ctctaccccc aatatacttt tcacattttt tattagtatt tttgttcatt ttgaatcaaa
     1801 agttacagtt ttgttaccat attttatata tgataccata gttatccata caactcaaac
     1861 aatatactta aagtttttat tcttcataaa ttcaaaacat tataacttat ttatttaatt
     1921 aagtaataaa ttcaaaataa tatatgttaa taagacattt aatccttgta aacagtatca
     1981 tttataataa ttttttatag tatgaatttc ccaatgctat aatcaaaaaa taaaataagg
     2041 catctcaatc taatttataa atgagatacc ttattcttat tattttgatt tcattattta
     2101 tatattttct aatatctaca ctatgtctta tacattgaca tattgtataa ccacatatta
     2161 tctaagtaaa cttttaatat tcattcttat attttattag ttcttagtaa ataagtttat
     2221 tttttctgct attttttcaa tcgcgttttt agctattcct tcacctattt gtactaactt
     2281 ggtagcattc ttaagtctta gctcaactac ttcttcttgt gatttagaaa gttcttctgt
     2341 agttaatact attggagctt ttgaactagc tgcaagtggt gctgctgtaa gtgcatcaac
     2401 taattgtccg tttccaccta cataaccatc ttttgcaaca tatgctttag ttgcacctgc
     2461 gtagtactta ttaattactt tagcattagt atcttttcta ttttctccag caagtctttc
     2521 aactttatta tttaaatcta tatcaaccaa tttatcttca acttcttttg atacagaatt
     2581 ttctccacct actatttcta attcttttcc ttttaatagt ttttcagcat ttgcatctaa
     2641 ttcgttacct gtaactatta ttggagcacc aaattgagca gctctagctg atatactcat
     2701 ggcatctgct tctccatttc cacctactac aaaagcagtc ttaaatgtat cttttaattg
     2761 ttttgctatt tctaaagaag tttcaaatct atcttctcca gaaactctct caactttgct
     2821 tacacctaat ttagaaagtt tttcttcagt ttcttttgat attttagatt ctccacctac
     2881 tatatatatt ttcttagaag atagattact tccaagtact cttaatattt cagattctat
     2941 ttctgatggt aatgaatctt ttttagataa taatattggt gcattttttt gagatgcaag
     3001 tggtgctgat gctaatccat caactatagc atcttctcca actaaaatta ttgcttctgc
     3061 ttctccatct ttgaatctct ctttactaac ttctactgct gttgtaaatc ttgaatcacc
     3121 agataaaact tctactttta attcttttcc gtcttttaca tctgtcaata ctttatgtaa
     3181 ctctctaact aacttatggt ttttacttga aacgcttatt gtttctagct tttcatttgc
     3241 tttacttaaa actatatcaa agcttgattt ttctggctta tctttatcat cactatcttc
     3301 ttgttcttga gtaaggattg cttcattttc aaatgttaaa gtatatccat ttttaacttt
     3361 attaactagt tctcttccta atgtagttaa tctaattcca tcaaataatt cttctgctgt
     3421 tatgtcttgt aaatctgcat ttttaactgt tattgtatgt aattcttttg attctatatc
     3481 actataactc ttttcataac cttcaaaagt accatcttct gatgttatta cttttgagaa
     3541 atctaaatgt tggtctccta catttaatgt aataacattt gttgaatctt ttgctctttt
     3601 tacttcaact gtacttgtag attttatagt tgctgtcaac ttgttatcag aatcttgtgc
     3661 taaagttgca ttgtaagcat taactgcatc tactatttct tgagcagttt tgtagttttc
     3721 aattttatag tcaactactt ttccacctaa aacttgatgt cctttatctt gaatagtaac
     3781 ttttaaactt tgcccttctg ttaatgagtt tattttattt tgtaattgag ttgcattagt
     3841 taagtttaca ttgtctactt ttatatcgta aactctatca tttacatttg ctcctgcttt
     3901 tgtatcttca aacttaactt ctaatgcttt tctaacctct tcgattagtt tagcagaatc
     3961 tttcgcactt actgtgtaat ttttatcaac attttctgtc acagtattat ctgcgaatat
     4021 tggtgcagct gcactcataa ctgtagctcc tgccattact attgccatac ttcttttttt
     4081 gttgctcaac atatttatgt cctccgaata ttgtttttga ttttttatat tcgtatttct
     4141 cacatataaa taatattata tataatatta tttttcaata tattttatat gtttacataa
     4201 tttactaaca caaacaatag taatatcttc ttgaacccct tcccccactt agctgtttat
     4261 gattatatcc aaagatagaa acccttattt tatagtgatt ttatattaga caatcatata
     4321 ctaacaaaat tattcaaact ccacttttat atactactat gcactaattt ttgctttctc
     4381 ttattactta aatatatatc actgaattaa tatctatctc catatattaa aatgcataa
//
LOCUS       DAMJVM010000156         4416 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.161, whole genome shotgun sequence.
ACCESSION   DAMJVM010000156 DAMJVM010000000
VERSION     DAMJVM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.161"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            75..374
                     /locus_tag="PC523_003360"
     CDS             75..374
                     /locus_tag="PC523_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476842.1"
                     /translation="MKWIKWYSFTCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKNNPVDGTTTIYYSYDNSTHIVRLSHPEDSSREINWDKVSNISFD"
     gene            696..2351
                     /gene="ilvD"
                     /locus_tag="PC523_003361"
     CDS             696..2351
                     /gene="ilvD"
                     /locus_tag="PC523_003361"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDF2476843.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSTM
                     EGVGACKDGTVSEEELEELAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGNTIGENIRDCKIENEDVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFSKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIEKRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            2692..3318
                     /locus_tag="PC523_003362"
     CDS             2692..3318
                     /locus_tag="PC523_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2476844.1"
                     /translation="MNREEKSKNSKEKIIQSAFLLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLENLVADNTLTANEKIIKLVVHSANDYTRRK
                     MLHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPQELAGMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDECGIQKFKDLFK"
     gene            3347..4159
                     /locus_tag="PC523_003363"
     CDS             3347..4159
                     /locus_tag="PC523_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2476845.1"
                     /translation="MFYNAKSCQMMIDSNTVNYIEFGSGKKILIILPGLGDGLSPLHG
                     KIQAIAFAFRYKRFAKDYKVYMFSRKNQITEKYSTRDMAKDQADIIKKLGIMKAKVMG
                     VSQGGMIAQYLAIDYPDLVEKLVLTVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDV
                     ANKSYSEKYLKKYRLFIPLLGKVGKPKDFNRFIIQATSCIEHNSFSELNKITCPTLII
                     GGANDKIVGNKASFHLAEKIKRSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
BASE COUNT         1711 a          488 c          798 g         1419 t
ORIGIN      
        1 ttttatcgta tgtataaata ttataaatat atggaaataa taatagtata ttttggtaaa
       61 gtaggaggaa ttttatgaaa tggattaaat ggtatagttt tacatgtata tgtattttta
      121 tagtagtagc tttttatatg tttatatttc ctaataaaat agaaactatt gatacttcat
      181 ctgcatatag ttttgttgaa aagaaagtac caaatagtgc tgtgtatcaa ggatacaaaa
      241 ataatccagt tgatggtact actacaattt attacagcta tgataattca actcatatag
      301 ttagattatc acatccagaa gactcttcaa gggagattaa ttgggataaa gtttcaaaca
      361 tcagctttga ttaatcttta atatttataa aagtctatat gtataaggta aatatattat
      421 taatcttatt catagttgag cactttctat tatagaagtg ctttttctga tttatagcat
      481 aaaatattac tcaacaattt gtaaaatata ttagtttata aaatatattg aaaagataat
      541 tatataatat gattgtgtaa gatattgata aaaaatcgta ataaattttc attagtatat
      601 gctaatattt ttttacaatt ttcgtaatta attaatagaa aaatatatta taataatatt
      661 atgaaaattt aaaaaagtaa gaaagaggta atattatgag aagtgatata aaaaaaggaa
      721 tagaaggagc tccaaagaga gcgttaatgt atggaatggg tcttactaaa gaagaaattg
      781 aaagaccatt aataggtata gtaaatgcac aaaatgaggt gattcctggt cacctacatc
      841 ttgatgaaat agcagaagcc gcaaaaaatg gagtaagaat gagtggtggt ttgccattag
      901 aatttcctgc tataggggta tgtgatggta tagctatggg gcatgtcgga atgaattatt
      961 cccttgcttc aagagaatta atagcagatt caatagaagc tatggctatg gcacatggtt
     1021 ttgatgcttt ggtacttata cctaattgtg acaagatagt gccaggtatg ttaatggcag
     1081 ctgcaagact taatatacca agtattgttg tgagtggagg tcctatgctt ccaggtaaaa
     1141 aaaatggaaa agtatacgat tttaactcta caatggaggg tgttggagca tgtaaagatg
     1201 gaacagtaag tgaagaagaa ttagaagagt tagctatgaa ctcttgtccg ggatgtggtt
     1261 catgttctgg tcttttcaca gcaaatagca tgaattgttt aacagaagct ttaggcatgg
     1321 gtataccata taatggtact gctgcgtcac attctggaga aagaaaaaga atagcaaaat
     1381 atgcaggtat gtatgttatg gagttactta agaatgacat aaaacctaga gatattttaa
     1441 cgatagatgc ttttaaaaat gctatagctg tggatatggc aatggctggt tctacaaata
     1501 cagtacttca cttacctgca atagcttatg aatcaggaat agagcttaac ttagattttt
     1561 ttgatgaaat aagtgaaaaa actccttgtt taacaaaatt aagtccaagt ggaaaacatc
     1621 atattgaaga tttacatatg gcaggaggaa taccagctat aatgaacgaa ctttcaaaaa
     1681 taaatggaat aaatttagat tgtaaaactg taacgggcaa tactataggg gaaaatataa
     1741 gagattgtaa aatagaaaat gaagatgtaa tacatacatt aaagaatcca tatagtaacc
     1801 aaggtgggct tgcaatattg aaaggaaatc ttgctctaaa tggagctgtt gtaaaaaagt
     1861 cagcagttgc agaagaaatg ttagttcatg aaggaccagc aagggttttt aattcagaag
     1921 aagaagctgt aaatgctatt tttagtaaaa aaataaacaa aggtgatgtt atagttataa
     1981 gatatgaagg tccaaagggc ggtccaggaa tgaaagaaat gttgtctcct acatcagcag
     2041 ttgcaggaat gggacttgat aaacatgtgg cacttcttac tgatggtcgt ttttcaggag
     2101 caactagagg agcatctata ggtcatattt ctccagaagc tatggaaggt ggcttaattg
     2161 gactagttga agaaggagat ataatttcta taaacatacc agacaaaaaa ttagaattaa
     2221 aagtggatga agttgaaata gaaaagagaa aattaaaatt taaaccttta gaaccgaaaa
     2281 taaagcatgg atacttaagt agatatgcta aattggtaac atcagcaaat acaggagcag
     2341 ttttaaaata gtaaactata tttatgcaaa ttagaaaaaa ttaagaaaat attaattgga
     2401 tatcttaggt gttatagcac taatgagata tcctttttat ttaaaataaa aacacagcaa
     2461 aatcttgatt atttcttaaa gtaatgcatc tctattaaag gaatatgagt ataacatatg
     2521 gatatatctt gtcaacattg ttgaggtaat acaacttttt aaagggctga tgcagactaa
     2581 ttttcgttct ccctaaacta tgaagaaatt aaaactcagc taattcttga cagaataaat
     2641 attaaaatac ataatgaaaa acaaacagac agtctgttta tggaggatga tatgaataga
     2701 gaagaaaaaa gtaaaaatag taaagaaaaa attatccaat cggcattttt gctgttttca
     2761 tctaaaggat atgattcaac atctacacaa gatattatca atttatctgg tctatccaga
     2821 ggtgcaatgt atcatcactt taaaactaaa gaagatatac ttagaagtgt cacaaaagaa
     2881 ctttattcac aaatgaataa ttttttagaa aatcttgttg ctgataatac ccttacagca
     2941 aatgaaaaaa taataaaatt ggttgttcat agtgcgaatg attatacacg tagaaaaatg
     3001 ctacattgta gctggttaga aaaaatacca tttgctttaa tagaggaagt tcgtaatctc
     3061 aataatgtag ttgcacccaa tattgctaag ataattaaac aaggtgttga aaataaagaa
     3121 ttttcttgtg aatatccaca agaattagct ggaatgctcg tttttagtat tgatatttta
     3181 cttgaccctg tattatttaa acgtgaatac agtgaagtat gtaataggtt ggatttttta
     3241 ttgtttatgt taaaaaaaat ggatattcct ctaattgatg agtgtggtat tcaaaaattt
     3301 aaggatttat ttaaataatg atagtaataa aagtgaggaa tagactatgt tttataatgc
     3361 aaaaagttgt caaatgatga tagacagtaa tacagtgaac tatattgagt ttggtagtgg
     3421 taagaagatt ctaattattt taccaggatt aggggatggt ttatctcctt tacatggaaa
     3481 aatacaggca attgcttttg cttttagata taagcgattt gcaaaagatt ataaggttta
     3541 tatgttcagt agaaaaaatc aaattacaga aaaatattcc acaagggata tggctaaaga
     3601 tcaagcagat ataattaaaa aacttggaat tatgaaagcg aaagtaatgg gtgtttctca
     3661 aggcggaatg atagctcaat atctggcaat tgattatcct gatttagttg agaaacttgt
     3721 gttaacagtt acttcttcta aacaaaatga taccatccaa aacgttattt gtagttggat
     3781 tgatatggct aagaaacaga attacaatga tttaatgatt gacgtagcaa ataaatctta
     3841 ttctgaaaaa tatttaaaaa aatatagatt atttattcct cttttaggaa aagtaggtaa
     3901 accaaaggat ttcaatagat ttattattca agcgacttct tgcatagaac ataattcctt
     3961 ttcagaatta aataaaataa cgtgtccaac cctaatcata ggtggtgcta atgataaaat
     4021 tgttggaaat aaggcttctt ttcatttagc agaaaaaatt aaaagaagcg aaatatttat
     4081 ctatgaggga ttgggacacg ctacatatga agaagcacag gatttcaacg aaagagtttt
     4141 agagttttta aataagtaaa tatatttgtt aaaaatatct atgccaatgt tgggaattta
     4201 atatggaaaa tggaaacatg aaaaggcact ggactggtac tttcaatata cctagccaat
     4261 ttcttatgta ttatctacta taaatgtaaa tataatttta atatgcttat gtgttggtgt
     4321 attagtaaaa cacttataca aaaagagaaa ttttgtaaat atagattata ccaacatttg
     4381 ttaacttatg aaaagataat caaaattttt atgagc
//
LOCUS       DAMJVM010000157         4366 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.162, whole genome shotgun sequence.
ACCESSION   DAMJVM010000157 DAMJVM010000000
VERSION     DAMJVM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.162"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            58..654
                     /locus_tag="PC523_003364"
     CDS             58..654
                     /locus_tag="PC523_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HDF2476846.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DINREEKINDIYKYLYKCKKIDFIVIDKKEKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKGDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
     gene            complement(751..1710)
                     /locus_tag="PC523_003365"
     CDS             complement(751..1710)
                     /locus_tag="PC523_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2476847.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNENIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDKVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(1744..2475)
                     /locus_tag="PC523_003366"
     CDS             complement(1744..2475)
                     /locus_tag="PC523_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2476848.1"
                     /translation="MVRSGFLLENICKKYLVDNKEHLVLDNVSLNISSEEITVILGES
                     GCGKTTLLRILAGLENATTGNVYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVSIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            complement(2468..3238)
                     /locus_tag="PC523_003367"
     CDS             complement(2468..3238)
                     /locus_tag="PC523_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2476849.1"
                     /translation="MFEKIHKKFKGYYIFFIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVVFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SRKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            complement(3323..4003)
                     /locus_tag="PC523_003368"
     CDS             complement(3323..4003)
                     /locus_tag="PC523_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014903004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HDF2476850.1"
                     /translation="MCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPSNGGGLGRALL
                     GHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESENLLAFSEQLN
                     YSVKLIGSGVISQKGLFTTHLANKTGQNQDVAIITDGNPLILEGPCCVDPDALVAWTG
                     REPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKTDSTPSDRMSS
                     GGLRIGID"
BASE COUNT         1467 a          768 c          492 g         1639 t
ORIGIN      
        1 tttatagtat aatctaatta cttgatatta attttcattt gaaggaggtt atcttttatg
       61 tcttgctgta attcacaatt ttgttgtaaa aacaaaattg aatcatcata tataaaatgg
      121 cttttagaag ttttaggtcc agttttctta ggctctaaac ctgctgaaat aataaatatt
      181 tcatttactg atataaatag agaggaaaaa attaatgata tttacaaata cctatataaa
      241 tgtaagaaaa tagattttat agttattgat aagaaagaaa aaggattaaa aatattattt
      301 gtaaacaaaa aggcattatc agaaaaatta aaatgtaaaa aaacagtaaa ctttttaaag
      361 tttttaggat acaaacaaaa tacgaatgtt aatgcttatt tagaacattt agtagataaa
      421 cttaagggtg atgtttttcc tgatgaaata ggtatttttt taggatatcc tcttaaagat
      481 gtgattggat ttatgggata tagtaattat gaagtttcta tgattaaata ttggaaagtt
      541 tatggtgaca caaaacaatc agaggacaca tattcaaagt ttttacttca ccgtaagaaa
      601 atgagaaaat tactagacta cataagtgta gataaaatag taagttgttt ttaatagtgt
      661 tttgtatgat aataaaaata caccctcttt taatatacag tttacataat aagtaagggt
      721 gtattttcta ctttaatatt ttaaaatatt ctaattctga ttgtacaaca tatctttaat
      781 atttattttc ttttgttgca ttccattaga catcaaaaat tcctgtgtat cttctaattc
      841 ttttatatcc ttatccgtta ttttagaact aaagtcatac caagaataca tttcttttgt
      901 ttcatctaaa gataaaccaa cttctttaga tacaacatcc atcactttgt ccttattttc
      961 attcatatat tttaaagttt cttcatgaac cttcataaat ctttttacta attcaggata
     1021 tttttcagca aagtcatcac ttacagcagt aactattatt ccacttacta gaccttctcc
     1081 atttacaatc attttggcac ctgaatttat cgttttcaat gccactggac ctgcaagcat
     1141 tgctacatca gcacttttac tttctaatgc agaagcagca ctaggaatat ccatatttac
     1201 atattcaaca tcatctatag tatagccttc tttagcaagt gcagcaatca acagttgatg
     1261 aagtatagtc ccttttggcc ctacaatttt cttacctttt aagtctttta tagattttat
     1321 attttcatta ttagttataa gcatatatcc ttttggtgaa cgactataaa tattcgtaat
     1381 tttaagacca acaccatttg acgcagctat tattgcagat gttcctccca atgcatgtaa
     1441 aaagtctagc tcacctgctg ctagtgcttg agtttgctct ggtcctgttg tcaattcatg
     1501 gaagttgact tttatattgt cctttttaaa ttcctttcca aataaatcat cctttttttc
     1561 taatatacta ggaacattaa gtggtgattt tacataagtt aaatttattt ccttaggtaa
     1621 actagcctct ttttttgcag gttcatcttt cttatcattt gttgttgagc atcctacagt
     1681 cccaaccaac accattatta aagatgtcat tactgctaaa atctttttgt ttcttttaat
     1741 caattaaaac tcccccaatt cccttaatat ttcttttttc aagtttatat aatagttttt
     1801 agttaaatct ctattatatt catcttcgac actaaactgt ttaatccttt tatttttact
     1861 aaaaacaatt attttctttg ctatttccat agcttcttca atattgtgag ttacaaatac
     1921 tacacctttt ttagtatttt tatggatact tacaacttct ttttgcattt ttcttcttgt
     1981 gaagtaatct agtgctgcaa atggttcgtc catcagcaaa atatctggat tgaaagaaag
     2041 tgctcttgct atacttactc tgtgagccat tcctcctgat aattcatttg gatatgagtt
     2101 tttaaatttt tcaagcttca tcatttttaa atacttgtct aaatcaactt tatttctatt
     2161 atccttttca gtatgtaaaa gtatattttc acttacattc agccatggca taagtctact
     2221 ttcttgaaat accattccta cttttggagt acatttttta tcattattaa aaaaatatac
     2281 gtttccagta gtagcatttt caagccctgc taaaattcta agtagtgtcg tcttcccaca
     2341 tccactttcg cctaatataa cagttatctc ttcactagat atatttaaag acacattatc
     2401 aagaactaaa tgctctttat tatcaaccaa atatttctta catatattct ctagcaaaaa
     2461 tccacttcta accatttaaa tcaacctgct tccctttgct aaattttgtt attatctttg
     2521 aaaacagata atctgaaagt atgcctaaaa atccaataac aaaaattcct accaagacaa
     2581 catcagttct agacatatct tttccatcag aaatcaaata tcctaatcct gaagaagctg
     2641 caataagctc tgctccaatt atagccctga aactataccc aagccctaag ctaagtccaa
     2701 caacaatatc cagcatagaa ttaggaatta ttattttata aaaaattctc ttcctagata
     2761 aattaaaaga tttacctact tctattaatt tattgtcaca attttttatg cctttcaatg
     2821 tatttaaaaa tattggaaaa aaggatgcca aaataataat tataatttta gattcttcac
     2881 ctataccaaa ccataaaata agcattggca ccaaagctag tggaggtgta cttcgcataa
     2941 actcaaagag tggcttgaaa tattcataca tttttttatt tactccaaat acaactccaa
     3001 gtggtatagc catcaatgaa cttattaaaa aacctatcaa tattcttctt acactggcat
     3061 aaacatttaa gaaaatagaa ccatcatata tcattttaat gaaagtatct aaaactattt
     3121 caggaggagg cagaatatag ctactccata tacccagaac gcatactatc ttccataaaa
     3181 ttaaaattat aaaaaaaata taatatcctt taaacttttt atgaattttc tcaaacatat
     3241 aaaactcctt ttaacatatc tacccctaat acccttttta ttttttaata tcctaaataa
     3301 taactaatct atatcataaa atttaatcta tgccaatcct taatccacct gagctcattc
     3361 tatcagaagg agtagagtct gtttttaaac cagacaatct ttcagatggc tgtataatga
     3421 ctatttgtcc aggttcaaca aattccatat gataagattc tcctgaagtc tgaccgataa
     3481 aagtcttcca tgacaatttt gacactttag gatatggttc tcttccagtc caagctacta
     3541 atgcatctgg gtcaacacaa cagggtcctt caagtattaa agggttgcca tctgttatta
     3601 tagcaacatc ttgattttga cctgttttat ttgctaaatg tgtagtaaaa agaccttttt
     3661 gagatattac acctgaacca atcaatttta cactgtaatt taattgttca gaaaacgcca
     3721 ataagttttc actctcaaca taaatggagt cacctgggtc aattgatatt acatctacat
     3781 gataagcatt ttgagctagg taaatttcac cttcaccttc aacaaccatt attggcattt
     3841 gttctcctgt caatgacctc tgaacatgac ctaataatgc tcttcccaag ccaccaccat
     3901 tacttggacc taacagcaat ttttcaaaat taaaatttcc tttaaaagct accatagcac
     3961 ctttttttgc aaaaaattta ccacttcctc ttgctataca cataagctca tttgtcacct
     4021 gaacctccca tgaataaagt cacagagttc ctgcttcaca gaattttgca tactaaaata
     4081 tgtcttacta attctccaca gtctatcccc ataatttcta cggttcttac ataagctatt
     4141 tattttgaat tgttattagt cttaatcctt cttttaatat atttagactt actttaatga
     4201 ttttatcata tttaatatac tatataatgc tgttgggaac aatttattca tcatctatca
     4261 taatattaac aaatgtaatt atttaaaaca attattcgaa ggttttatat ttatttttaa
     4321 ataacatatt taagaaccgt aagtcttaac ttttatcatt tttgag
//
LOCUS       DAMJVM010000158         4354 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.163, whole genome shotgun sequence.
ACCESSION   DAMJVM010000158 DAMJVM010000000
VERSION     DAMJVM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.163"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(811..1032)
                     /locus_tag="PC523_003369"
     CDS             complement(811..1032)
                     /locus_tag="PC523_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476851.1"
                     /translation="MILKKVIISGICIFTCLLASSIPTFANELQYENIEQYATIEYAN
                     DSMERMSYIKSGSCGLTISNGIATIRSSV"
     gene            complement(1103..1816)
                     /locus_tag="PC523_003370"
     CDS             complement(1103..1816)
                     /locus_tag="PC523_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HDF2476852.1"
                     /translation="MEILSNKKDNFDITDDMLQEIIPIATKIILDDLPSDDELNHIFS
                     KSFERKMKKLIKQQKRSSLTNKIIFYSKRTAIVFLIVLASLFTITMSSEALRTRLYEL
                     VIRVYEDLTSFVFSTNDGEESLNLKIIEPKYIPNDFKEVDRVDNFIINYKNKNNIEIN
                     YMFCEISSNSIILDTENAKVENISINGNRAKYIEKGTSIQLFWNDKNCIYLLSTDCYK
                     EDLLKKYKNELIKMGKSIK"
     gene            complement(1800..2342)
                     /locus_tag="PC523_003371"
     CDS             complement(1800..2342)
                     /locus_tag="PC523_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2476853.1"
                     /translation="MLIYLAMLELEEDKIKFEKIYEKYRQIMFYVANKILKDDHLSED
                     AVHNAFLRIIKNIDKIDEVDSPRTKAFIVIIVERIAIDFYRKRKREKVSDIEEEYKNR
                     EVNFSIEDKVCESNLTIALAKLNESYFQVLSLKFQYGFSNKEIANVLNLGEENVYKRI
                     QRARKKLKEILEEMEDGNIE"
     gene            complement(2589..2771)
                     /locus_tag="PC523_003372"
     CDS             complement(2589..2771)
                     /locus_tag="PC523_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476854.1"
                     /translation="MKKRFYKVFLKTIPNLIIVILSLALSFYTNTFDIYLKILLLIPL
                     AYVLLFIYYYIKTKKD"
     gene            complement(2799..3410)
                     /locus_tag="PC523_003373"
     CDS             complement(2799..3410)
                     /locus_tag="PC523_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476855.1"
                     /translation="MKNKRINSILSIIMTTVIISLSFVTTANSMEKTESDNNIKIELS
                     DKEVEELHNYWPEDKIFSDEKIDEILIKTGQFTQEELDKINQEVDDYAKKVNQNSRVK
                     YINGYNKYRTFVKNGKKYLYIYVSGKTLKKIKAGADLSSTFGGFLPKIWVGLAVVVIG
                     KIITTNMNGINTNYGIVLSYIEDKWNFQGATYTYRYDHWFYQT"
     gene            3546..3740
                     /locus_tag="PC523_003374"
     CDS             3546..3740
                     /locus_tag="PC523_003374"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDF2476856.1"
                     /translation="MQDLCKYTQNLSLKTTNQFLAAFLALVFSNNKVTNKVRQFLDLT
                     LKLIPYALVSTEYSEEKMNL"
BASE COUNT         1564 a          625 c          393 g         1772 t
ORIGIN      
        1 attttataag ctttttaaga gactgtaagt aaatttatgt aaaaataaat ttacttacag
       61 tctcgttatt tattagtata aatatttaca tgaaataact taaaacctca aaatatgtcc
      121 accttatacg ctatacttaa tattaagatt tttatactct ttataaagta ctgaaattta
      181 tagataaagt tttaaattaa tattaaagaa aataataact tattttttag gtcctttgtt
      241 cttatttctt ttgatatact cttttgctac tttatcaaat cgcccaagat aatccataaa
      301 aacatcacca gaagttaatt ttcttgaagt ataagaattt attactattt tcatttcaat
      361 cctcccatga attttattga tatatagtat tatagcgtgc ttctgattgt attcggattt
      421 ttttgaaatt tattaataac ttataagtac tttattcctt tctaaaaata tcaatcacaa
      481 gtataagtat tattattatt aataataaat tagcagactg gattaatata attggccatt
      541 caaaatatat tatttatagt cacctcatct tatatgtaca atatattttt attatctttt
      601 tttactactt attatatttt tgatttcaga gttagctttt accatagcat gaactctgta
      661 atatccttta ctgatttttt gttttttaga aattgaacaa gattattttc ttgaagcatt
      721 ccatgtatta attgtttacc atgtctttct attattatca ctcttttgta gtcttacaac
      781 tattgatgta ctttttacat tcactcttcc ctatacagaa cttcttatgg ttgcaattcc
      841 attagaaata gttaagccac aactaccaga ttttatataa ctcattcttt ccatagaatc
      901 atttgcatat tctatagttg catattgttc aatattttca tattgtaatt cattggcaaa
      961 tgtgggtatt gaagatgcca acaaacatgt gaaaatacat ataccactta taattacttt
     1021 tttcagaatc ataaaaaaca cctcctcatt cttttgtata actataaaac gaataaagag
     1081 atgctttctg gacactagaa aattatttta tactttttcc catttttatt aattcatttt
     1141 tgtatttttt caaaagatct tctttatagc aatcagtact taataagtat atacaattct
     1201 tatcattcca aaataactga atagaagttc ctttttctat atattttgct ctattaccat
     1261 ttattgatat attttcgacc ttagcatttt ctgtatctaa tataatagaa tttgaactta
     1321 tttcacaaaa catatagttt atttcaatgt tatttttatt cttataatta ataataaagt
     1381 tatccaccct atctacttct ttaaaatcat ttggaatata tttaggttct ataatcttaa
     1441 gatttaaact ttcttctcca tcattagttg aaaaaacaaa agaagtcaaa tcttcatata
     1501 ctcttattac caattcatat aaacgtgtac gtaatgcttc gctactcata gtaatagtaa
     1561 ataaactagc taaaactatt aaaaatacaa tagctgttcg ctttgaataa aaaataatct
     1621 tatttgtaag actacttctt ttctgctgct taatgagctt tttcatcttt cgctcaaaac
     1681 tttttgaaaa aatatgattt aattcatcat ctgaaggaag gtcatcaaga ataatttttg
     1741 tcgcgattgg gataatttct tgaagcatat catccgtaat atcaaaatta tcttttttat
     1801 tactcaatat ttccatcctc catttcttct aatatttctt ttaatttttt tctagcacgc
     1861 tgaatccttt tatatacatt ctcctctcct aaatttaata catttgctat ttctttattt
     1921 gaaaaaccat actggaattt taaagaaagt acttgaaagt aactttcatt aagttttgca
     1981 agagctatag ttaaattact ttcacatacc ttatcttcta tagaaaaatt tacctctcta
     2041 tttttatatt cttcctcaat atcagatacc ttttcccttt ttctctttct gtaaaagtct
     2101 atagcaatcc tttctactat tataacgata aaagccttcg ttcttggact atctacctca
     2161 tcaattttat ctatattttt aattattctt aaaaaagcat tatgtactgc atcctctgat
     2221 aaatgatcat cctttaatat tttattggcc acataaaaca ttatttgtct atacttttca
     2281 tatatctttt caaactttat tttatcttct tcgagttcaa gcatagctaa ataaattaac
     2341 atttagaatt cctccaatat aaaaattctg aattaatatt ttaaagttca attataacat
     2401 aaatatataa ttaactctac ttaaatagaa tataattatg cttaacaagc tttttaaata
     2461 tacctagtaa aaaggccctt caaatccatt ttggggatta taagggcctt aactttacaa
     2521 ctaattctca aagttaaact tttaaattaa ttattttcgt atatatctaa ttccataaaa
     2581 aataaaaatt aatccttttt tgttttgatg taatagtata taaacaaaag tacatatgct
     2641 aaaggtatta aaagcaatat ttttaaataa atatcaaatg tattagtata aaatgataat
     2701 gcaagtgata atatcacaat tatcaggttt ggaattgttt ttaaaaaaac cttatagaat
     2761 ctttttttca tattattcct cctaaaaaag catgtctttt aagtttgata gaaccaatgg
     2821 tcatatctat aagtatatgt tgcaccttgg aaattccact tatcctctat gtatgataaa
     2881 acaatcccat aattagtatt tataccattc atattagttg ttattatctt tcctataact
     2941 actacagcaa gacctaccca aatttttggc aaaaaacctc caaaagtaga tgaaaggtct
     3001 gctccagctt ttattttctt taaagtctta ccacttacat aaatatataa atatttctta
     3061 ccatttttga caaaggttct gtacttgtta tatccattta tatactttac acgagaattt
     3121 tgattgactt tcttagcata atcatcaact tcttgattaa ttttatccaa ttcttcttga
     3181 gtaaattgac cagttttaat taatatttca tcaatttttt catctgaaaa aattttatct
     3241 tcaggccaat aattatgcaa ctcctctact tctttatcag acaactctat ttttatatta
     3301 ttatctgatt ctgttttttc cattgaattt gctgtagtaa caaatgataa agatattatc
     3361 acagttgtca ttattatgga taaaatgcta ttaattcttt tattcttcat ttaaatcctc
     3421 ctaactataa taaatttata aaccttgtac atatataacg tatctaaact cgattattgg
     3481 acaaaaattt taaaaatatt tatattttta aaattaaatc ttctagaaac tttaaatgta
     3541 aatggatgca agatttatgt aagtatacac aaaatttatc attaaaaact actaatcaat
     3601 ttctagctgc tttcttggca ttagtatttt ctaataataa agtaacaaat aaggttagac
     3661 aatttttaga cttaacatta aaactaatac cttatgccct tgtatctaca gaatatagcg
     3721 aagaaaaaat gaacttatag atagatttga taaaaagaaa atacatataa atatataact
     3781 tttagtgatg taaaaaaata tataataaaa atgcccttaa aatctatttt ttggatttta
     3841 agggcattaa ctttatctag aattagtaaa tttctatatc ataaaatttt tttaattaat
     3901 acattatttt gctataaaac attattcaac tatctatttt caataaaatc taaaattgat
     3961 gaggtgtgta gtaagtaaaa aaaatttata attattatta taaatttttt tgctctaatt
     4021 gaaagcaata attctgataa aaaatgtagt taatataaat aattgtgtaa accgtaagat
     4081 aatagataac gattaaattc aataatactt taaaacttaa tgtaattcta atcctattta
     4141 tgatatttgt ttgtatttat tctatttttc atgaaagtta taattatatc tactccaaca
     4201 atcaataata aaacacagta tctatataac gattttccta gtccatcaag gtaaaaaata
     4261 cttataagac taattattat tataactaat gtaaacaaaa tactctttgc aaatgataaa
     4321 ttattatttc taaaaaatac aggtataaga aaaa
//
LOCUS       DAMJVM010000159         4307 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.164, whole genome shotgun sequence.
ACCESSION   DAMJVM010000159 DAMJVM010000000
VERSION     DAMJVM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.164"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            <1..238
                     /locus_tag="PC523_003375"
     CDS             <1..238
                     /locus_tag="PC523_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2476857.1"
                     /translation="VRQLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKNLNIR
                     EWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            444..1295
                     /locus_tag="PC523_003376"
     CDS             444..1295
                     /locus_tag="PC523_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2476858.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDISIAISYSGESKEVIKSIENAKLKGCKVICMTKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNIDNA"
     gene            1906..2940
                     /locus_tag="PC523_003377"
     CDS             1906..2940
                     /locus_tag="PC523_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF2476859.1"
                     /translation="MKKIRKLATKLLITTMVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDVIYKSLSHPLPFDKTAYPTVGAVAQTQKKPAPPGKHTMLPHQEGA
                     KVVGKFGDIKDKHVMYEGVRCVVINSSVNTVTFMGSESNLDPSELTIAVIGFMNTPYG
                     MFALAPESKPVEYPFEYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIEITDEKANTG
                     LGQIMYIRYE"
     gene            complement(3056..4075)
                     /locus_tag="PC523_003378"
     CDS             complement(3056..4075)
                     /locus_tag="PC523_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476860.1"
                     /translation="MLKLSLLYLFKGGLHLNNINSLKVIKLSLGCSLAIFIAWLLKLE
                     YSMVAGVIVLLTVKDTKRETLKGSIGKVYGFLLCTIFSYLCFNILGYNLISFSVFIFI
                     IIPLCFLLNIQDVIAMCVVIASHYFLQGETSIKWILNEAGIFAVGTGIGVLINMYIPT
                     NIHKIHEVQKKLQEEVSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNI
                     NNTLLSDTRFFLEHMDIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGHTEFE
                     AETGNLLLEELKRLMLSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKNAQMLRD
                     NNFYK"
BASE COUNT         1694 a          558 c          700 g         1355 t
ORIGIN      
        1 tgttcgtcaa ttagaataca aggctaattg gcatggtaga caaattgtaa aggtaggtaa
       61 attctttgca agttcacaaa tatgcaataa atgtgggtat aaaaacgagg aagttaagaa
      121 cttaaatata agagaatgga tttgtcctag ttgtaatgaa actcacgata gagatataaa
      181 cgcaagtata aatatattaa aagaaggatt aagactaata acaattcaaa ataaataact
      241 tatgtaagaa ccgtaggaac tacggggata gcctgtggag aattagtaag acatatttta
      301 gtatgcaaaa ttctatgaag caggaaccct gtgactttag tcatgggagg ttcagatggt
      361 gagaccagtg ttgactccag atttaggagc atgtttactt gctaagaaga aatattatag
      421 taaataggta gaggtggtag agtatgagtt gcattttaaa attaaaacag atatatgaag
      481 atttaacaga agtagataaa aaaatagctg attacatact taataataca gaggctataa
      541 gtaagctttc tgtatcagaa ttggcgtcaa actcaaaaac gagtacagca agtatagtta
      601 ggttttctag aaaaatggga tattcgggtt ttggagattt aaaaattgag atagctaaag
      661 atttgatggg caaagaaaat gaatacacat atgtagatga aaactatgat tgtaatatag
      721 atagtgttat aaacaaaatt acaaacaaaa acatagagac tatcaaccaa acaaggttgt
      781 tgaatgaaaa agatataata aaagaagcag ttgaacagat aataaaggct aaaaatgtat
      841 acatatttgg agtaggtggt tcggctttag ttgcactaga tttacagatg aaacttttaa
      901 ggataaataa acaggcattt acctctttag atagtcatac acaacttatg gtatcttcaa
      961 atgtagataa agaagatata tctatagcaa tatcttactc tggagaatca aaagaagtaa
     1021 taaagtctat agaaaatgca aaattaaaag gatgtaaggt catttgtatg actaaatata
     1081 gtgacaatca tcttagtaag attagtgatt taaagctagt agtaccaaat atagaaaaaa
     1141 ggcttagaga aggagctatt tcttcaagaa tagcaatgct tacattgata gatataatat
     1201 atatttctat aattcaagaa aacttaaatg aggcagaaca gaaactacaa gaaacaaaaa
     1261 ggatacttga ttatttaaat attgataatg cttaaaacta ggttatatat caactaaaca
     1321 aataagttag actataagcg taagtcaata agaaatatat aaatatctaa aatattgact
     1381 tactcaataa taatatatag cattttataa tacaaatgag ttgtcttaaa atttattttt
     1441 tttaatcaaa ttttaagttc aactcctttt ttatattatt atagatatat cctatatttt
     1501 cccaaaaaac gtattgaaca tacaaattat aggaggtata atgttatgga gagagaatat
     1561 atgaaatgta tacaaaattg attttaactg tattattgat aattctcttt actcttacca
     1621 ttactaatga ttgaagacag tagtatttat cataggtcaa agattccgaa tatattaatc
     1681 atcatcattt tctcaaatta acagcaaaca tactgatttg cgagttgtat tgtatacaaa
     1741 ttatctagaa aatatctatt agattttcca tgaaatttaa atatttcgcg aacgtatgaa
     1801 cgtagaaaca tacatgttga caatccacat ttttaaatcc caaatgagct ttattaatgg
     1861 tagagaatag gaagtaacaa tattttataa aggagaatac taagtatgaa gaaaattagg
     1921 aagttagcta ccaagttatt gattacaaca atggttttaa tctcaggtat gagtatgaca
     1981 gtttatggta tgacagctaa agaggtaaca gcaaagacgc caaaaagcta cgtcactgga
     2041 accaatagcg tatatggtcc gaagttgtca caagcccaac tgaactcagt agcacaagca
     2101 acagcagatt ttatgaacaa gaaaatcaca aagaacatga ctacagatgc taagatttta
     2161 gttgcctaca atcacattaa aaataacaca acatatgttg actggaatgc agtagaggga
     2221 gcaaatactg cctatacatt ggttactaaa aaaggtgcat gttcaggaat ggctaggagc
     2281 atgaaagctt tgtgtgatgc tatgggaatt gaatcttatt atgtacacag cacatcaaat
     2341 gaccaccagt ggaatcttat ccgttttggt gatgggcgtt tgtatcatgt agatattgat
     2401 gctaataaat cagcaggaaa ggatgtcata tacaagagtc ttagccaccc attaccattt
     2461 gataagacag catatccaac agttggagca gtagcacaaa cgcaaaaaaa accagcacca
     2521 cctgggaagc acactatgtt accacaccaa gaaggggcaa aggttgttgg aaaatttgga
     2581 gatataaagg ataaacatgt aatgtatgaa ggtgttcgat gtgttgtaat taattcatca
     2641 gtaaatacag ttacatttat gggtagtgaa tcaaatttag acccatcaga gcttacgatt
     2701 gctgtaatag gatttatgaa cacaccatat ggaatgtttg cattagcgcc agaaagtaaa
     2761 cccgttgaat atccttttga atatgataag ccattttctt tagattttag tggtaaaaat
     2821 cgttctggtg cagaattagc gaagtactgt aaaaaaaatg atgtgactat taaaatagaa
     2881 atcactgatg aaaaagctaa tactggactt ggtcaaataa tgtatattcg atatgaataa
     2941 tgatttaatc atgacagtag aataagttat gaacattgcg ccctcttact aggggcgcat
     3001 ttaatattat atctgatatt ttgtagtcag gtcaactgct ggatttctcc cctttttatt
     3061 tataaaaatt attatctcta agcatctgag catttttacg attaactaaa aactgtttta
     3121 actcagtcaa gcataggaat aaaatagccc tattttcaaa ttctgttctg tcaacaggta
     3181 gaggttggtt tttcatacta agcattaatc tcttcaattc ttcaagaaga agattgcctg
     3241 tttcagcttc aaattcagta tgacctattt tatgtatgaa agcagagagg atatgagctt
     3301 gtggtggtgt agaatttagt tgagatacgt aactatacaa attttctaag atatcccgtt
     3361 gactttttat aatgtccata tgttctaaga agaatctagt atcactcaat aaagtattat
     3421 taatattatc gtaagtttca gaaatactgc tgtcaataag tgaatttaat gtatataaat
     3481 ctctactata tccattttct tttttagggt ttacaataat atctgcaatg tctataagta
     3541 caatactcac ttcttcctgt aacttttttt gaacttcatg gattttatgt atatttgttg
     3601 gaatatacat atttattaag acacctattc cagttcctac tgcaaatatt ccagcttcgt
     3661 ttaaaatcca cttaatagat gtttctcctt gaagaaaata atgggaggca atgactacac
     3721 acatggcaat aacatcttga atatttaata aaaaacaaag tggtataatt ataaatataa
     3781 atactgaaaa tgaaatcaaa ttatatccta aaatattaaa acataaatag gaaaaaatag
     3841 tacataaaag aaaaccataa acctttccaa tagagccttt aagagtttct cttttagtat
     3901 cttttactgt aagtagtact ataactccag caaccattga atattctaat tttaatagcc
     3961 aagcaataaa tatagcaaga gaacatccaa gagacaattt tattacttta agtgaattaa
     4021 tattattcaa atgcaaacct cctttaaaca aatatagtaa agataatttt agcataaaac
     4081 taattttgtt taaaatatag ttaaccatat tttatttaaa aattattata caaatcaata
     4141 aaaagataag taagtgtgga tacaaagata cctttttaat gtaaaatatt gtagaataca
     4201 aaatattata atgaacacaa aataattata atctattaaa taaaaaatac tttaaattac
     4261 aattagagta gttaataaaa taactgaaaa tatatctatt attatta
//
LOCUS       DAMJVM010000160         4269 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.165, whole genome shotgun sequence.
ACCESSION   DAMJVM010000160 DAMJVM010000000
VERSION     DAMJVM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.165"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            347..2446
                     /gene="nrdE"
                     /locus_tag="PC523_003379"
     CDS             347..2446
                     /gene="nrdE"
                     /locus_tag="PC523_003379"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDF2476861.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDREALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSMDFIKNIYEIIDKQKFEFQSYMSANKFYQNYALK
                     SNDGKKILETYNDKVLIVALTLGNGNKDLVLNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMNKWYDKLVNDNDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDIGIIKMSNLCCEIFQYQTHSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIEKNQT
                     FEGFDKSEYAKGRNSKVLSKYYEQSYLPKSEKVKALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAIAPTQSISYVQNSTSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNILYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2459..3430
                     /gene="nrdF"
                     /locus_tag="PC523_003380"
     CDS             2459..3430
                     /gene="nrdF"
                     /locus_tag="PC523_003380"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDF2476862.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKIWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNSEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEILEEKMYSILYRLMENEIEYTNIIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3507..4133
                     /locus_tag="PC523_003381"
     CDS             3507..4133
                     /locus_tag="PC523_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2476863.1"
                     /translation="MKYEWRKRDKELYLPKKNPAIIEVPEQKFIMLKGNGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKCLDVLIKEDLIYT
                     LMIHQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYIHHEIYISDARKVSPESLKTVLRFEVEREV"
BASE COUNT         1712 a          456 c          783 g         1318 t
ORIGIN      
        1 tagtataata tatttttagg ctatttagag atgatttgaa aatttaaata tatcataata
       61 agcttatatt gttttatatt tgagttgata aaaatttctt taaacaaaaa taatgtcagg
      121 tatacaataa taaaagttta gatataaaaa aatttgtgta tatactatat gtagtatata
      181 atagataaag cactgactat atgtagtgct tttgtttttg aaaaatactt attatatcct
      241 ataattatag attagttttg ggatttcaac taataagtac aaaaagaaat aataagcaaa
      301 ttaaatgaaa ttatagcaca agattatgtt aaaaggacgg taaataatga attggataga
      361 gcttaacaat caagtgataa ttaaaaatga acaaggtaag tatcagttgg aaaaggatag
      421 ggaagcctta agtagttaca tagaagaatt tatacaacca aagttaagaa aatttaataa
      481 cctagaagaa agattgaagt atttaataga tgaaggttat tattcaaaag aagtaataaa
      541 tcagtatagc atggacttta ttaaaaatat atatgagatt attgataaac agaagtttga
      601 atttcaatct tatatgagtg caaataagtt ttatcaaaat tatgcattaa agagcaatga
      661 tggaaagaaa atattagaaa catataatga taaagtgtta attgtagcat tgacattggg
      721 aaatggcaat aaagacttag ttttaaactt ggcagataaa atagtaaaac aagaatttca
      781 accagctaca cctacttttt taaatgctgg gagaaaaaga gctggagaaa tggtatcatg
      841 ttttttacta tctgtagaag atagtacaga aggtatatcg tatgctatat cttcatcaaa
      901 tcacttatca aagattggtg gaggagttgc actgaattta tctaaactta gagcatctgg
      961 agaatctatc aaagatatag aaggtgctgc tggaggtgtt gttggagtag ctaaaatgtt
     1021 agaacaatca tttagttatt tcaatcaaat gggagctaga caaggttctg gtgcagtgta
     1081 ccttactgta ttccatcctg attttgaatt attgatggat actaaaaaga taaatgcaga
     1141 tgaaaaaata aggcttgcta cattatcatt aggagctata atcccagata aatttatgga
     1201 gttggcagaa aaaaatgaag tagcatatgc attttatcct catactgtgt ataagaaata
     1261 tggcgtttct cttgatgaga tagaaatgaa taagtggtat gataaattgg ttaatgataa
     1321 tgatataaga aaaaaagaaa taaatcctag gcagatgttg acaaaaatag cacagatgca
     1381 acaagaaagt ggatatccat atgtagtgta catagataca gctaatagag aacatacatt
     1441 aaaagatatt ggaattatca agatgtctaa cttatgttgt gagatatttc aatatcaaac
     1501 acattcagaa atagaggggt atggaggcaa aaatgaatgg ggacaagata taagttgtaa
     1561 tctgggttct ttaaatatag ccaatgtgat ggataataaa actatagaaa gtacagttga
     1621 aacagcaata agagcattgt catttgtagc agatagcaca gatataaaac cagtgccaac
     1681 agtaagcaac agtaacagca agtctcactc cataggacta ggagctatga atttacatgg
     1741 atacttagta agagaaaata ttttatacac ttcagatgat gctatagatt tttctaatgt
     1801 attttttgca atggttagat actattcaat taaagcttct atgaaaatag ctatagaaaa
     1861 aaatcaaact tttgaagggt ttgataaatc agaatatgct aagggaagaa atagcaaggt
     1921 tttatcaaaa tactatgaac aatcttatct gcctaagtcg gaaaaggtaa aagcgttatt
     1981 tgaagggatt tatataccaa caaaagaaga ctggacaaaa ttattagatg aggtaaaaga
     2041 aaaagggata tataatgcat atttaatggc tatagcacca acccaaagca ttagttatgt
     2101 tcaaaattct acatcaagta taatgccaat tactgagcca gtagaagtaa ggacatatgg
     2161 agattctact acaatatatc caatgccatt tttaacaaat gataatatat tgtattatca
     2221 atcagcatat aggatggata tgagaaaggt tatagattta gtggcaacag ttcaaaatca
     2281 tgttgaccaa ggtatatcaa caacactatt tgttacagat gaaaagacaa ctagagatat
     2341 agctagacat tacatatatg cctataaaaa aggtttgaaa agtttatatt acacaagaac
     2401 aaagatgaca agagacactc atgagtgtct agtgtgttca gtatagggag agaaatatat
     2461 gacatttaat ttacataaaa tacataatgc agttaactgg aatagagagg aagatggatt
     2521 tacacaggct ttttgggaac aaaatgtaaa acaattttgg cttcctgaag aaatatctgt
     2581 ttctaaagac ataaagatat ggaatgaact aagtaataaa gaaaaggaat tatataaaaa
     2641 agtattgggt ggtttaacat tattagatac aaaacagggg aataatggaa taccatctat
     2701 gatgagttta acagaaaact tacaaagaaa agctgtcttg tcttttatgg ggactatgga
     2761 agaaatacat gcaaaaagtt attcttctat atttatgact ctactttcta attcggaaat
     2821 agatgaacta tttgaatgga tagaaacaga accaacatta caaagaaagg cagatttagt
     2881 tttagctcaa tacgaaaata ctacaaatca agagggatta tacttatcaa tggttacaag
     2941 tgtattttta gagagtttct tgttttattc aggattcttt tatccattgt atctatctgg
     3001 tcaaggaaag atggtagcta gtggagaaat aatatcgctt attcttagag atgagtcttt
     3061 acatggtaag tacataggat tgcttgcaca agaaatatac gattcatttg ataaaatgga
     3121 taaggagata ttagaagaga agatgtattc tatactttat agacttatgg agaatgagat
     3181 agagtataca aatattatat acagagaaag tggtcttgaa aaagaggttg tgaatttttt
     3241 aaaatacaat gcgaatagag ccttagaaaa tctagggttt gagaagttat atactgtaga
     3301 tgctataaat ccaattgtat taaatggtct tagcactgaa actaaaacac atgatttctt
     3361 ttcgactaag ggtaatggat accaaaaagg agtgtatgag gaattagagg atgaagattt
     3421 tataatataa tttataatta tgacataatg ttgtcacttt tagcatgata atatcaatgt
     3481 atgaaatata tgtggaggta ttttttatga aatatgaatg gagaaaaaga gataaagagc
     3541 tatatttacc taaaaagaat cctgcaataa ttgaagtacc agaacagaaa tttatcatgt
     3601 taaaagggaa tggagaccca aattctaaag aatttactga agcaatagga gtactttatt
     3661 ctttggcata tgcaataaaa accatgccta aaaagggaat tgtcccagaa gggtattttg
     3721 actatactgt ttttccttta gaaggcatat gggataaagg agaaaaaagc aaatgtttag
     3781 atgtacttat taaagaagat ttaatttata ctctaatgat acatcagcca gattttgtta
     3841 cacaagacct gttggataaa gctactgata tagtaaagaa gaagaaacct cataatcttc
     3901 ttgaaaaagt tagatttggg tctattaatg aaggtctatg tgttcaaatg cttcatatag
     3961 gttcatatga taatgaacca gaaagctttg atattatgaa cgaattttgt aaaaaaaata
     4021 aacttataag aaaatcttac atacatcatg agatttatat aagtgatgct agaaaagtat
     4081 cgcccgaatc attgaagacg gttttacgtt ttgaagtaga aagggaagta taaatatttt
     4141 gaataattta taagagttat aaaaagtgaa gtctgttgag acttcatttt ttgatgcagt
     4201 tttaaaatta aatttgatta aaaaatagct atgcacactt gtgactttag tcatgagtta
     4261 gtagctaaa
//
LOCUS       DAMJVM010000161         4002 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.166, whole genome shotgun sequence.
ACCESSION   DAMJVM010000161 DAMJVM010000000
VERSION     DAMJVM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.166"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            58..687
                     /locus_tag="PC523_003382"
     CDS             58..687
                     /locus_tag="PC523_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HDF2476864.1"
                     /translation="MEFNELFNFKYLIIAVIFLISVTMLILISHINILIMADINNKDI
                     CLKLNIKYMFNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSVYRLLKKI
                     KIHELYSNINFGTENIGLTSSVYVLINTLYGNLFNIIDAEKMYLNVNPDFTKDYVLGN
                     IRIHIKSRIKDLFNIIIMINKIMNKNKGNKEGDSNESNRFNTESYGNNS"
     gene            641..1069
                     /gene="ytfJ"
                     /locus_tag="PC523_003383"
     CDS             641..1069
                     /gene="ytfJ"
                     /locus_tag="PC523_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HDF2476865.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            1307..1747
                     /locus_tag="PC523_003384"
     CDS             1307..1747
                     /locus_tag="PC523_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2476866.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            1763..3214
                     /locus_tag="PC523_003385"
     CDS             1763..3214
                     /locus_tag="PC523_003385"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDF2476867.1"
                     /translation="MNIKCSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLVENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEVYKSS
                     DVNVLNLNLNSDEIGSFAEGILLGNYKFDRYKTKSKNFERKEVDTITIFTEKDVECQI
                     KKAKILANSTIIARNLVNEPSNVIYPKTLAIETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIINSMANKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKVKGATGEPVRTLYYLAEI"
     gene            complement(3306..3974)
                     /locus_tag="PC523_003386"
     CDS             complement(3306..3974)
                     /locus_tag="PC523_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HDF2476868.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSTNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFKVNRYALRDNLAI
                     CFIYFSFIFLLNTLTNSQYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
BASE COUNT         1677 a          366 c          674 g         1285 t
ORIGIN      
        1 ttaaaataaa tatggataat taagtataaa tagtaatttt ttgatgataa tataaatatg
       61 gagtttaacg aattatttaa ttttaagtat ttaataatag ctgtaatttt tcttataagt
      121 gtaactatgt tgattttaat atctcatata aacatcttaa taatggctga tataaacaac
      181 aaagatattt gcttaaagct taatataaaa tacatgttta atttaataaa tataaataga
      241 caactttatc cagctgaaaa tagtaaaaat aatgataaaa aagaggggat gaagaataat
      301 atagattcaa gtatattatt agctgatgac cttttgagcg tttatagatt attaaaaaag
      361 ataaaaatac atgaacttta ttcaaatatt aattttggaa ctgaaaatat tggcttaaca
      421 tcttctgtat atgtattgat aaatactttg tatggaaatt tatttaatat aattgatgca
      481 gaaaaaatgt atttaaatgt aaatccagat tttacaaaag attatgtttt gggaaatata
      541 aggatacata taaaatcaag aataaaagat ttatttaata ttataataat gattaataaa
      601 attatgaata agaataaagg aaataaggaa ggtgatagta atgagagcaa caggttcaat
      661 acagagtctt atggaaacaa ctcttgaaac aattaaaggt tctatagatg caaatactat
      721 tataggagac cctataaaaa cagatactac agtggttgta cctatatcaa aggttacaat
      781 tggttttggt ataggaggag gagaatattc aaaaggatat gatgataaag atagggaaat
      841 tgaattaaaa aatgaaaaat cagatacgaa ttttgctgga ggtagtgcag gggctatatc
      901 tgtacaacca gtagcgtttg tagtagttga aagtggagaa actagaataa tgagtttaga
      961 tagcaatatc aatctagtgg ataatatatt atctataact cctagagtat tggagaaaat
     1021 tcaaaatata tcacaaagta ataagaatca agataaaaac aatatgtaag aataaaatat
     1081 gtatatggtt ttaaataaag tagtggggtt agtataaatc ccactatttt gctgtgtact
     1141 ttaaacattt atatctaata agattttagt ttaaaacaat ttaaatagat aataaagttg
     1201 atatatagta taattataaa aagaaaagaa gtattatatt atagatatat aaacacattt
     1261 gagatataag agggagttat aaatcattag ttttgaggtg aatgttatga gcgataaaga
     1321 attatctatt ttgttgttag aaagtatgaa taattttcat aatttaataa agattattaa
     1381 taacgaaaga tataaaaaag ataaggttct tacagaaaga cagttctttg ccttagtaaa
     1441 aataagaaaa catgataaaa tagagcttaa aaacctaagt agagatttac atgtatctac
     1501 ttctagtttg tgtatactac ttaataaatt ggttgagcaa gagtatgtat atagagaaga
     1561 agatagtaga gatagacgaa atacttttta tggaataact aaaaatggag aaaagatatt
     1621 ggataatgaa atactaaagt ttgtgtctat aataagtgat aaaatggatt gcttagatat
     1681 agataataaa gataaattat ttacttcttt ggaagaatct aaaaatataa tagaacaact
     1741 attttaaaaa ggagaattta ttatgaatat aaagtgttct aaagaaatat taaaaagttt
     1801 agaaaatgct ataacagtag tagctctatt tgagggagaa gatgtttcta cgacatttga
     1861 taaaataaat atgataagca gaaattttaa ttctaaagaa aatagtatag atactgtaac
     1921 attggttgaa aataacaatt tggtaaatgt ttgtcttgtt ggttttggta agagagagtt
     1981 attgaataga gaaaaaatta gaatcatagg tgggaatttg tgtaaaaaat taaaaaatat
     2041 attgaaaaat gaagtatata agagtagtga tgttaatgta ctgaatctta atttaaatag
     2101 tgatgaaata ggttcttttg cagaagggat attacttgga aattataagt ttgataggta
     2161 taagacaaaa agtaagaatt ttgaaagaaa agaagttgac actataacta tatttactga
     2221 aaaagacgtt gagtgtcaaa taaaaaaggc taaaatatta gctaattcaa ctataatagc
     2281 tagaaatcta gttaatgaac catcaaacgt aatataccca aaaacattag caatagaaac
     2341 agtcaaactt ggtgcagagt ttggatttaa tgttgatgtt tatgaagaag aaaaaataaa
     2401 ggcattggga atggatgctt ttttcgctgt ttcaagaggt tcaattaata aaccaagatt
     2461 catagtaatg agatactttg gagacaaaga aagtacggat atattaggtc tagtaggtaa
     2521 aggattgact tatgatactg gtggatattc tttaaaatca aatgcttcta tgctagatat
     2581 gaaaaccgat atggctggtg ccgcgagtgt tataggtgct atgtgtgcta tatctcaaag
     2641 taaattaaag aaaaatgtta tagcagtagt ggctgcatgt gagaatgctt tatcaggagg
     2701 ttcatacaaa cctggagata taattaattc tatggcaaat aaaactattg aagttttaaa
     2761 tacagatgct gaaggtagac ttacattagc tgatgcaata tactacatta taaacaatga
     2821 aaaagtgaca aaggtggttg atgtagccac attaactggt gctgccttaa cattgttagg
     2881 caatgttgca actcctatag taacaaataa tgatgatttt tattgtgaat tagaaaaagc
     2941 cgccacttta tcaggagaaa gagtatggaa gatgccaatt tatgatgaat ttaaagatat
     3001 gataaaagga gaagaagcag accttaaaaa cactggtggt aaaaatgctg gatgtataac
     3061 tgcaggagct tttattggtg aatttgttgg gaatactcct tggatacata tggatatagc
     3121 agggacaagt acatcatcaa aatcaattgg atacaaagta aagggggcta caggagaacc
     3181 tgtgagaaca ttgtactatt tggcagaaat ctaaaacaac aattatagaa tataatttat
     3241 aaagccacgt ggatattttt gtaatttaag gttatctatg tggctttatt ttttatttaa
     3301 gtaaattatt tactcttttt ctttttttca agtataaaag tatctctatt aaataaaaga
     3361 gcaggtatgt acataatact agtaacaaaa ataaatgtaa aaatagcagt tgtactatgg
     3421 cttaaaaaac cataatagta ttgtgaattg gtaagtgtgt ttagtaaaaa tatgaatgaa
     3481 aaataaataa aacatatagc taagttatct ctaagtgcat atctgttaac tttaaatttt
     3541 ctaacatcaa ccatatatat atctgcataa attattaagc acttagagaa gataaatata
     3601 aaatatatta agctaggaaa ttcataaccc aagtttgtag aaaaaataag ttctccaact
     3661 gaacaaacta aactccaaga aaaaaatata ttaaaaagat gatactgctt aaaaatcagt
     3721 atagctatac acagatatat gcaaatataa ttgattccaa ttggtaaaga atttagggta
     3781 ctataattac ctgaagaaat aagtaataat tgctctagag ccataattat tacaagcaat
     3841 gtacaaatta tcttctcgac tgtatagcta taaggtaaaa tatttttggt taattttgga
     3901 caatagtata aaaataatcc aaatataaag agacttacaa attgttcact agaaaaaaga
     3961 aaaattttaa acatataaca ttctctccta taataaattt aa
//
LOCUS       DAMJVM010000162         3795 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.167, whole genome shotgun sequence.
ACCESSION   DAMJVM010000162 DAMJVM010000000
VERSION     DAMJVM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.167"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            27..695
                     /locus_tag="PC523_003387"
     CDS             27..695
                     /locus_tag="PC523_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476869.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     KGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKGEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYKDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGILYKNDGNKQAGIWEDDEQCKSLDFKDLSNNEISKVSQNRLNNN
                     KLRV"
     gene            854..1360
                     /locus_tag="PC523_003388"
     CDS             854..1360
                     /locus_tag="PC523_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDF2476870.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLNLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            1488..2504
                     /locus_tag="PC523_003389"
     CDS             1488..2504
                     /locus_tag="PC523_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDF2476871.1"
                     /translation="MGYYNDNDNNKIRRKKISNSKDSNKKSKHSYTSANKKNKSSKTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPDDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGGSTIDMQVSKNLLTS
                     DDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVNE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQLIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            2860..3564
                     /locus_tag="PC523_003390"
     CDS             2860..3564
                     /locus_tag="PC523_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HDF2476872.1"
                     /translation="MKKFVIEDTFWNLFPNAKIGVIICNDIDNSIKDEDYKKIILQGE
                     EEALKYLEDSEFSKNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENLFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALSELAKIVQEKLGGK
                     CKISILDIDNKEVSVD"
BASE COUNT         1617 a          317 c          668 g         1193 t
ORIGIN      
        1 ataaaatttt taaagggtga ttttttatgg aaaatataac tagttatttt acaacaatac
       61 tatgtgtatt tatatgttta tcatctgtat ttatatttac tcaactagct agagtattta
      121 ttaataagaa gaagattaat caaaaaatta aatcaagaaa aggatttagg tatgatagag
      181 attttattga agctagaaga gaagaaatac atataaagga taacaataat aataaaaata
      241 agagtaacaa taagaaatta aagggagaaa aagtatttaa atatgataat ggagatttat
      301 ataaaggtga gtttgttgat gggaagaaaa atggatttgg aatatatatt ttttcaagta
      361 aggaaaagta tgaagggtta tggaaagatg acaagatgca tggaatagga aaatatactt
      421 ataaagatgg ttcaatatat acaggtgaat ttaaatacgg attaaaaaat ggtttgggga
      481 aattaacata tcccaataat gatatttata agggatattt tttagataat aagaaaaatg
      541 gtaagggtat attatataaa aatgatggaa ataaacaagc aggaatatgg gaagatgatg
      601 aacaatgtaa aagtttagat tttaaagatt taagtaataa tgaaataagt aaggtttctc
      661 aaaacagatt aaataacaat aaattaagag tttagttatt ctaataaaaa aatctttatt
      721 tatgatggaa tatagaatat gtttatgctg taatttgtaa aatcttccta tatcatataa
      781 aattaagtat tgattagtct attacataaa tatagaatat aaatatatcc atttgataat
      841 aaggagaaga gatatgaaag agtatgtagt tgtggattta gaaactacag gattagatcc
      901 atataaaggt tgtgaaataa tagaaatagg aataacagaa ataaaaaatg aacaaattgt
      961 aaaaaactat tcaaggttga taaaacctaa ggggataatt tcttcattta taacagaatt
     1021 gacacatata agtaatgaaa tggtagaaaa tgaagaacct ctagaattag ttttacctag
     1081 atttagaaag tacataggtg atagaactat aattgcacac aatgcgaaat ttgatttaaa
     1141 atttttaaat tactatctta gaatgttaaa tttagaacca ataaacaatt atatatgtac
     1201 agtagaatta ttaaaaaaat gtaaaagtta taaaggaaaa aataagaaac ttgaaacagc
     1261 ttgtgcttat tacaacatag agaatataaa tgctcataga gctgatagtg atactttagc
     1321 aactgcaaaa ctatttttga aaataaaaga tgaatattaa tcataaatat agagaaaatt
     1381 agtgaaaata tcctttataa aattgaccct taaacttaca attttgttct ttgatttatt
     1441 ttgtagaaca atgtataatg taccttatat gaagaaggga gactaaaatg ggttattaca
     1501 atgataatga taataataaa ataagacgta aaaaaattag taacagcaaa gatagtaata
     1561 aaaaatctaa acattcttat acttcggcaa ataaaaaaaa taaaagttct aagacaagtt
     1621 ctagtaaaag aatgagcgaa aaagtagatt cacagaatct tataaaaaga gatagagata
     1681 gttttacaag agataatgta agacctttaa atacagatta taatcaaaga gaaagaagaa
     1741 gagtcgagta taaacaaaga gagaaaaaac taaaaagtaa aaaaagaaaa aaacaaaaaa
     1801 ggattagaat agttagggca ttgttatcta ttatgttagt tgcactaatt atattttcag
     1861 tggcaggtgc aatatttaca gtttcagcta taaaagggtc accagaagtc actaaaaagt
     1921 taattaggtc taaatatata agtagtgagg ttgtaagtat aaaacaaatg ccagatgatt
     1981 taaaacatgc aatagtttcc atagaagatg agagatttta taagcataaa ggagtagatg
     2041 ttatttcttt agttaggtca gtactaaaca atgtgtttac agatacaaca caaggtggaa
     2101 gtactataga tatgcaagtt tcaaaaaatc tgcttactag tgatgataaa actatgaaac
     2161 ggaaaataag ggatatgtat aatgctaagc aaatgaataa gattatgact aaggatgaaa
     2221 ttcttgaagc ttatttaaat aatatgtacc taggtaaaaa tgcatatgga gctgcaaagg
     2281 gagctgaagt ctactttggt aaaaaagtta atgaattaaa tttagctgag tgtgctatgt
     2341 tagctggtat gacaaataat cctgcaaggt atattgacca tggagaggct aagaagagac
     2401 aattgattgt attgtataag atgcatcagc ttggttatat aactgataag gagtatagaa
     2461 tagcaaaagc tgatgacact ccatttaaat ctgaaattga ttaaaattgt aaagatgtgg
     2521 tatttagaac agttcaaaca aatgaaaatg taataaaaaa ggtgatttgc cacccatctg
     2581 tcaccataat tataatctta tggcaaattg ttttaattca caaattagat aatagaatat
     2641 tgttttaagt gctatagtat gctaaaattt aaaattgtat tttataaatt aaaaaaagtt
     2701 ataacttagt tttctagggt tccgcatttt ataatggtca ggtccaagag aaaacgcaca
     2761 attattgtgt acacggaagg ataaaagcct gggagatatt taataatatt tctataggct
     2821 tttttatttt tatatatttt aaatttaagg aggtttttaa tgaaaaaatt tgttattgag
     2881 gatacatttt ggaatctatt tcctaatgcg aaaattggag ttattatttg taatgatata
     2941 gataactcta taaaagatga agactataag aaaatcattt tgcagggaga agaagaagca
     3001 ttaaaatact tggaggattc agaatttagc aaaaaccaag tgattaaagt atggagagaa
     3061 gcatttaaaa aatttaaaac gaaaaaagga gcaaggtctt ctattgaggc attattaaaa
     3121 agagttcata atggaaataa tataggaact attaaccctt tagtggatat ctacaatttt
     3181 atctcattaa aatatgcatt accttgtgga ggggaggata tagataaatt tataggagat
     3241 attaggctaa ctagagcaat tggtaatgag gagtttatcc cactagggac agatgaaaat
     3301 ttatttccat atgaaggaga aataatctat aaagatgatg gtggagcaat ttgtagatgt
     3361 tggaattggc gtgaagcagt gagaacaatg cttactgaag atactaataa cgccttttta
     3421 tgtattgaat tagtagatga aagtagattt aaagaatttg aaaatgcttt aagtgaatta
     3481 gctaaaatag ttcaagaaaa attgggtggg aagtgtaaaa tctcaatttt agatattgat
     3541 aataaagaag tatcagtaga ttaatctgga ggtgctgttg taatgggcta ttctagttat
     3601 aaagatactt atatttaggg taagaaggtc tattttagat gcaacgatta tcaaaggata
     3661 tgatatagaa aataaaaatg tggaatataa aaagccttta aaataagaaa aaattgtatt
     3721 ttaaaggctt ttattatact attttttatt tgcacaatta acaataaaaa tatattgtta
     3781 attataatat tttta
//
LOCUS       DAMJVM010000163         3744 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.168, whole genome shotgun sequence.
ACCESSION   DAMJVM010000163 DAMJVM010000000
VERSION     DAMJVM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.168"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(178..747)
                     /locus_tag="PC523_003391"
     CDS             complement(178..747)
                     /locus_tag="PC523_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDF2476873.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
     gene            complement(819..1427)
                     /locus_tag="PC523_003392"
     CDS             complement(819..1427)
                     /locus_tag="PC523_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2476874.1"
                     /translation="MDSTDKKGLKSPEIMIINGSPRINKNCSFIANEVKAMLEEYGLS
                     CKIFNIYDMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYILNKPSIDRNKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNDTDNVSYMSNQEFKDIIYKNLKRYIDSI"
     gene            complement(1435..3198)
                     /locus_tag="PC523_003393"
     CDS             complement(1435..3198)
                     /locus_tag="PC523_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HDF2476875.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYLTEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDTFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNETIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYAIKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDASYE
                     NNTISMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            complement(3241..3708)
                     /locus_tag="PC523_003394"
     CDS             complement(3241..3708)
                     /locus_tag="PC523_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476876.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSIFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
BASE COUNT         1214 a          662 c          344 g         1524 t
ORIGIN      
        1 atatatataa ttttaccata ttacatcata tttttgtaag tttttattac attttttata
       61 ataccccttt attttataaa ttaatctaat cgattaaatt tatcctatag aaatttatta
      121 catttaaaaa tatgctagca aataaaatat ctactagcat aaagtctatt tataatatta
      181 atcaactatt aattctacta agtctccatt tttaacgcct gctgcatttc cctcttccat
      241 atcaacatgc atttctagat taaagctatc actagctctt actaatacat tttcaaatac
      301 taatgctctt ggtccagatg ttttaacttt tactatttgt ttatcagtta caccaaattt
      361 agcagcatct tcattgctca tatgtatatg tctagaagca actataactc cttctttcat
      421 ttcaacttct ccagcaggac ctactagttt aacacctgga gttccagcta tatcaccaga
      481 ttcttttatt ggtgctttca ctcctaatgg gaaaccatct gctaaagata tttcaacttg
      541 agtatgagct ctagctggtc ctagtactct aactcctttt aaagttcctt ttggtcctac
      601 taaatccact ttttcttcac aggcatattg ccctggctga gataatggtt taaatttagt
      661 taactcatga cctgcaccaa ataatacgtc tatatcagct tgactcaaat gaacatgttt
      721 attggataat gctattggta atttcattga aatttcctcc ttttaattat ttaaattatt
      781 gcaatacaat tatacataat attacctaca aaatcaaatt atattgaatc aatatatctc
      841 tttaaatttt tatatattat atctttaaat tcttgattgc tcatataaga tacattgtct
      901 gtatcgttta aatatatatt gtgtaaaagt ttagtattta tactcttaaa gaaaaaatcc
      961 ataacaatct gtccaccttt aaactgtgaa tcatatgatt tactaccccc tactgcaata
     1021 tacattccta tccgtttctt gtttctatca attgagggct tatttaatat atatttactt
     1081 gcatatatag cttgtgttct atcaacaact gtctttaatt tagctgatat tgaatcaaaa
     1141 tgtacaggac ttattactat agttcctttt gaatcatcaa acattttata taatggcttc
     1201 atatcatcat taaacttaca ataccctgtt ttttcacaaa atccacatgc tgtacaatat
     1261 tcaatatcca tatcgtatat attaaatatt ttacagctta gtccatactc ttctagcata
     1321 gcttttactt catttgctat aaaactgcaa tttttattaa ttcttgggct tccatttatt
     1381 atcataatct ctggactttt aagtcctttt ttatcagttg aatccataat caatctacct
     1441 atcctttaac ataactatta aaatatcatc atcataatta tcttttgatg agaaattctt
     1501 taaatccaac tttagattct ctactattaa ctgttgattt aatctaaagt tttcttttag
     1561 aaagttttct aatctgttta taccatactc ttcttttgat gaatttttta tctctaaaat
     1621 tccatcagta tacatacata ccattgcata atcttccatt gaaatagtat tattttcata
     1681 actagcatct tccattattc caataggtat acccttttta caatttaaat tattttctat
     1741 tgtaccatcc ttctttacga ctacaggact atagtgacct gcattagaca ctgtaatcat
     1801 gttttcttta gtatctataa tacctatcaa acaagttgta aatactccca tcttgtcaaa
     1861 ttcatcatat aacatcctat ttagatttgt cattatttct cttggtgtat tgtattgatt
     1921 acataatacc ttaaaagcac cttttatcat tgcaacaaca taatttgata ccattccatg
     1981 tcccattaca tcagcaacaa tataaactat atgttcgtca tctactttta tagcataata
     2041 aaaatctcca cctactactc ttgctggttt atgataaaaa taaagttctt tctcatcatt
     2101 acaatccatt ttacctttat ccataataag ctgttgctgt ttatttaata tgttcagctc
     2161 attttcaaga actttgtgtt ttacttcctt ttcattatgt tcatataatt gcataccaac
     2221 agaaacttgt ttggacagta tactaagaat attcaaatca tcactagtat agtttttact
     2281 atttgcacaa actatacatc ctatagtctc atttttatcc tggagtttgt aatatatata
     2341 actttccaaa tttattcctt tttttgagca tggttttaaa ttatttagat accctcccaa
     2401 tgattctttc tttaaaaacg tatcaatcat aggatataaa tttgttttct ttttttgctc
     2461 aacattataa cagctaacta tagcatcctt attatgatga ctatttataa ttattaaagc
     2521 ttctttacta ttagtaatat tgcatgcttg tttacttaaa ttttctaaaa agccatcaat
     2581 actcttatta aggtatagtt ttttagtagc ttcatttatg gcacttattg agcctcttag
     2641 tttatcaatt aaaatttgta cattattatt tttattagtt atttctattg ataaacttaa
     2701 gagtgatgca acagatgata taaagtctat atcttgttct gttaaataat catcctcttt
     2761 taaaaaacaa gtcataaatc caacaactac attatttatt ataaaaggaa aaactattct
     2821 cccaatataa ccttcagctt ttgctaaatc tcgttcacct tctgctctgg aatcttcaaa
     2881 tacattttcc acatatatta tctttttttc atttacagcc tcatgtatat actctggata
     2941 cgtgtcaaaa tcaatttttg cacctttaag actatttatt ggaaaaattt gaggtacata
     3001 ttctaaggtt tgtgaacata ctaagtaggc atatttagaa tcatttttat aaaatagatt
     3061 cacacatgcc tttgtagggt gaacaacttc taacattttt tctattatgt catcttttat
     3121 attaaaaaaa tctgtagatt cagttaccat agcggatatc tccactaaat tcttcatttt
     3181 ataactgcca ttagtcatac tcaatttctc cccctatata ttaaactatt tattagtatt
     3241 ttaacataca ttctttatag taaaaacaaa aacaacgaca aaactagcaa ctaatatcca
     3301 gttaaatcct gttaataatc ctataagaag taatgtaatt aatgcattta gacctgttct
     3361 acctttttca tccataaaag ttattttaga gatgtcgata ttatttttca cattatttat
     3421 atgttctttt acttgttctt ccatatttct tttatatagt atataaacta caccaactaa
     3481 aaatactcct atacctagaa ccatgtttaa attaaaagct accataagca atattgaacc
     3541 aaaagccaac tttaaaagct taaaaaatat actatctatc tttaaggtta tatttctgat
     3601 ttctttacat tcgtctctta aagaatatcc acttgaatct aagttattaa tctttgtttt
     3661 tacaatttca gaataaaaaa gttttattat tccatttaac attttcatca ctcctaaata
     3721 ttttacatat atttgtaagt ttta
//
LOCUS       DAMJVM010000164         3708 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.169, whole genome shotgun sequence.
ACCESSION   DAMJVM010000164 DAMJVM010000000
VERSION     DAMJVM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.169"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            140..1078
                     /locus_tag="PC523_003395"
     CDS             140..1078
                     /locus_tag="PC523_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDF2476877.1"
                     /translation="MNKRMKLIPYEINENPRGAKNKFPYGIKQMNVRGMWDEGYTGKN
                     IVVGIIDTGCDVSHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVIQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMGNKLAIMSGTSMSAPYVSGSLALIKEWARDEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNTGKR"
     gene            1592..2020
                     /locus_tag="PC523_003396"
     CDS             1592..2020
                     /locus_tag="PC523_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2476878.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFIAEGVKSKGKTPEVLDVSLLKPS
                     DLKEENKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMREW
                     EERMQNAGAVIIGGEGITAMEEPDEEAKDECIELGKMLAE"
     gene            complement(2173..2967)
                     /locus_tag="PC523_003397"
     CDS             complement(2173..2967)
                     /locus_tag="PC523_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase"
                     /protein_id="HDF2476879.1"
                     /translation="MEKYANMIKEKLNSGKVLCGVAVSLSDSVVSELIGISGYDFVWI
                     EGEHGALDRKDIQQHMIAAHAGGAASLVRLVNSDPDIVKPIMDMGTDIICFPFINTVQ
                     DAQKAVAACTYPPNGIRGYNPQRASYYGNMNYFDYVKNANNETLKMMLIEQKAGYDNL
                     EEIVKVKGVDIIALGPGDLSLDMGFSGYMDRIEIKDMISNATDICKKYKMPFVTFPSI
                     EKNSIETWVNIGSNMLCFAQDTTFITYVINQLLSFYNEIVPIKKQH"
     gene            3116..3484
                     /locus_tag="PC523_003398"
     CDS             3116..3484
                     /locus_tag="PC523_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2476880.1"
                     /translation="MKYSMTDVSKITEISSSAIRFYEKKGLLLPINRKPSGVRYFYEE
                     DINRLMFIKELKNAGLSIKDIHECFHFCDMGDDTIDKRIQLFQQHKQNIKNKIYELEN
                     CMDRIDEKIYYLKVKKMCKK"
BASE COUNT         1459 a          410 c          619 g         1220 t
ORIGIN      
        1 aataaaatat gattcctaaa aataaaaaat atatcataaa tataaataag tagatatagg
       61 ttttaatttg atttataaac ctattataaa ataaaattta tgatttttat aagcaataaa
      121 atttttagga ggatatgtga tgaataaaag aatgaaacta attccatatg aaataaatga
      181 gaatccaaga ggtgcaaaaa ataagtttcc ctatggaata aagcaaatga atgttagggg
      241 aatgtgggat gaaggttata ctggtaaaaa tattgtagtt ggtataatag atacaggctg
      301 tgatgtatcc catcctcttt taaaaggaaa aataattggt ggggcaaatt ttagtgatga
      361 cagtaatgga aataaaaata tatatgaaga ttttaatggg catggaactc atgttgcagg
      421 cattatagct gcatctaatt ataataatga agttatggga gtagctccag attgtaaatt
      481 attaatagca aaagcattaa ataaagatgg tactggaaca tatcaaagta taattaatgc
      541 tattaacttt gctgtaaata acaaggttga tattatatct atgtctcttg gaggaaacaa
      601 agatgataag aatttaaaaa atgctgtcat acaagcagta aaaaataata tttctgtagt
      661 gtgtgcagca gggaataatg gagatggaga ctctagtaca agtgaatata gctatccagc
      721 aagttatgct gaggtgatag aagtaggtgc aataaatgag aactatttag ttgaaaagtt
      781 tagtaattca aatactacaa tagatttggt ggctccagga agaaatatta tatcaactta
      841 tatgggtaat aaacttgcta ttatgagtgg tactagtatg agtgcaccat acgtatcagg
      901 ctcattagca ctaattaaag aatgggcaag agatgagttt gaaagagatt tagatgaagc
      961 tgaattgtat gcacaattaa taaaatgtac gagggcactt ggaataccta gaacagaaca
     1021 aggaaatgga tacttatatt taaatcttta taaatacaag aatactggta aaagataatt
     1081 tataatttta taataaaata aatagggtat atagttaacg caaatgataa aaattaacta
     1141 agtgtatatt ttgaaataga tatatgaaaa tatttaataa taaggtctat agacctgtta
     1201 tgcaattaga ataataaaaa atatacagta ttatagatag cacatctgaa gaaagatgtg
     1261 ttcttctata tgtctgtata gatgctgtat ataaaaggta aattctagat tgaagttaag
     1321 aaggtataag attatttata atacgaacaa gaatattaag cgtgagaaat attcttataa
     1381 aagatttaga aatataatgt gatattatag gtaaataaag agtatagaaa ataaatgata
     1441 tatattatca ataggtattg aatttcaaaa tcatttgtga taatatatat ttagaaaaat
     1501 aagagatttt agataaagga attttttatt tttagaattt agttagcgat tgctaacttt
     1561 cgattaatta aaaatactac aggaggtaag aatgagtaaa atatatattg tttattggag
     1621 tggaacagga aatactgaaa aaatggcaaa ttttatagct gaaggtgtga agtcaaaagg
     1681 caaaacacca gaggttttag atgtaagttt gttaaagcca agtgatttaa aagaagaaaa
     1741 taaatttgca ttgggttgcc catctatggg agcagagcaa ctagaagaag gatatatgga
     1801 accatttgtg tcagaattag aatctatggt atcaggtaaa caaattggat tatttggttc
     1861 atatggttgg ggaaattgcg aatggatgag agaatgggaa gaacgtatgc aaaatgctgg
     1921 tgcggtaatt ataggtggtg aaggaattac agcaatggaa gaaccagatg aagaagcgaa
     1981 agatgagtgt atagaattag gcaaaatgtt agctgaataa atttgtatac tataaaaata
     2041 gtataagtag taactaatga taatagcagt gtataatact aagagagact aatattatga
     2101 tacataagaa aacaccctta atagagatag ataaaatatt agtctctttt agtataaaat
     2161 tagatttata atttaatgtt gttttttaat tggtacaatc tcattataaa aagacaataa
     2221 ttgattgata acataagtaa taaaggtagt atcttgtgca aaacaaagca tatttgatcc
     2281 aatattaacc catgtttcaa tactgttctt ttctattgaa ggaaaagtaa caaatggcat
     2341 tttatatttt ttgcatatat cagtggcatt agaaatcata tcttttattt caattctatc
     2401 catgtatcca gaaaatccca tgtccaagga taagtcacca ggacctaatg ctataatatc
     2461 aacacccttg acttttacta tttcttcaag gttatcataa cctgccttct gttcaattaa
     2521 catcattttt aatgtttcat tgtttgcgtt tttgacatag tcaaaataat tcatattccc
     2581 ataataagag gctctttgag gattgtatcc tcttataccg tttggaggat aagtacaagc
     2641 tgctactgct ttttgtgcat cttgtactgt attaataaat gggaaacaaa taatatctgt
     2701 tcccatatcc attataggct taacaatgtc agggtcactg tttactaatc gtaccaaaga
     2761 agcagcacca ccagcatgtg cagctatcat atgttgctga atgtctttac gatctaaagc
     2821 accatgttct ccttcaatcc agacaaaatc atatccagat ataccgatta actcacttac
     2881 aactgaatca gacagtgaaa cagctactcc acataagacc tttcctgaat ttagtttttc
     2941 tttaatcata tttgcatatt tttccatata catcatcctt tcttttgtca gtttactttt
     3001 acacttcaaa gaatacaact tcaatctaac ttgaagtcaa tacttttttg ttgactacag
     3061 tataaggtat ttttataata ttcttattcc tataaaattt atggaggtaa agattatgaa
     3121 atattctatg actgatgtat ctaaaataac agaaatttct tcatctgcta ttcgatttta
     3181 tgaaaaaaaa ggtttattac ttcctatcaa tagaaaacca agtggtgttc gctatttcta
     3241 tgaagaagac ataaatcgtc ttatgtttat caaagaatta aaaaatgctg gtttatctat
     3301 taaagatatc catgaatgct ttcatttttg tgatatggga gatgacacaa tagataaaag
     3361 aatccaatta tttcaacaac ataaacaaaa cattaaaaat aaaatatatg aactagaaaa
     3421 ttgcatggat agaatagatg agaaaatata ttacttaaag gtaaaaaaga tgtgtaaaaa
     3481 ataatcctta tagaataagg aaataatttt ttgatttttc taatataagt agtaaattca
     3541 aatggcaaca tttgagaaaa gattttttac aatatatttc tacgaagaat tggacaatgt
     3601 tctatataac tatttatcta tacaatgaaa agaaaattat gatagtatat attaatgtaa
     3661 gtagtaaaaa tatttttgtt tgtaaatatg agtattttta aacactat
//
LOCUS       DAMJVM010000165         3696 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.170, whole genome shotgun sequence.
ACCESSION   DAMJVM010000165 DAMJVM010000000
VERSION     DAMJVM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.170"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            248..397
                     /gene="rpmG"
                     /locus_tag="PC523_003399"
     CDS             248..397
                     /gene="rpmG"
                     /locus_tag="PC523_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDF2476881.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            423..644
                     /gene="secE"
                     /locus_tag="PC523_003400"
     CDS             423..644
                     /gene="secE"
                     /locus_tag="PC523_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDF2476882.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTG
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            664..1206
                     /gene="nusG"
                     /locus_tag="PC523_003401"
     CDS             664..1206
                     /gene="nusG"
                     /locus_tag="PC523_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HDF2476883.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1254..1679
                     /gene="rplK"
                     /locus_tag="PC523_003402"
     CDS             1254..1679
                     /gene="rplK"
                     /locus_tag="PC523_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDF2476884.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1750..2448
                     /gene="rplA"
                     /locus_tag="PC523_003403"
     CDS             1750..2448
                     /gene="rplA"
                     /locus_tag="PC523_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDF2476885.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2500..2628
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2671..3177
                     /locus_tag="PC523_003404"
     CDS             2671..3177
                     /locus_tag="PC523_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDF2476886.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3235..3600
                     /gene="rplL"
                     /locus_tag="PC523_003405"
     CDS             3235..3600
                     /gene="rplL"
                     /locus_tag="PC523_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDF2476887.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
BASE COUNT         1492 a          446 c          739 g         1019 t
ORIGIN      
        1 ctttactaaa ctgatggatt aaaaggaata acaaaaagag gggttatgct cctctttttt
       61 taacatagaa atacaaaaac attgacacag cataggattt tgtgataact tatacaaggt
      121 aatgattata ggaagttaat aatatcttga atactgtaga tttttaataa atacatattt
      181 taaagatatt atattcacat atattaacaa tatatattat tgtattacaa agttggaggt
      241 gtatcagatg agagttaaag taactttagc atgtacagag tgtaagcaaa gaaactacaa
      301 cactactaag aataagaaaa ataatccaga cagaatagaa ttacaaaaat attgtagatt
      361 ctgtaaaaag catacaactc ataaagaaac aaaatagttt tttataagga tgtgagtatg
      421 gaatggctgc ccaaaaaaat gacggtgcaa aaactaaaaa aaggtttagt ttgtttggat
      481 acttaaaaga aactaaacaa gaattaaaaa gagttacatg gccgacaaaa aaggaactgt
      541 ttaaaaacac aggtatagtt ttaactgtag ttatttcatg cactatatta gtatggggta
      601 tagacactat attatctggt gcactagcgt tattactaaa atagacgaaa agaggtaagt
      661 taaatgtcag aattacaaga agcaagttgg tatgtagttc atacttattc gggacatgaa
      721 aataaagtta aagctacaat tgagaaagca gttaaaacaa gaggtatgga agactgcata
      781 agacaagtag ttgttccaac agaagaagta gtggaaacta ctaaaactgg aaaagaaaaa
      841 actagacaac gtaaagttta tccaagctat gttttggtta aaatgataat aactgatgaa
      901 tcttggtatg tagtaagaaa tactaaggga gttacaggtt tcgttggacc tggttcaaaa
      961 ccagtgccat taagtgaaga cgaagtcaaa gctatgggga tagatacaac agaccctaaa
     1021 gtagtaaata gcgatattga ttttgaaata ggagatacag taaaagtatc tcaaggacca
     1081 tttagtggac aaataggtaa catagaagaa attgacttag agaatagaga agtcaaagta
     1141 tgcataaatg catttggtaa aaggactcta tttgtaatag agcttgaagg tatagaaaaa
     1201 atctaatact atatttataa tattatagaa aaatctgagg aggtgcgctc aaaatggcta
     1261 aaaaagttat aggtcaaata aaattacaaa tacctgcagg aaaggctact ccagcgccac
     1321 cagttggacc agcattagga caacatggtg ttaatataat gggattcaca aaagaattta
     1381 atgcaaaaac tgcagatcaa gctggaatga taataccagt tgttataact gtatatcaag
     1441 atagatcatt cagttttata acaaaaactc caccagctgc agttttaatt aaaaaagcat
     1501 taaacttaaa atcaggttct ggagaaccaa acaagaaaaa agttgctaaa atgacttcag
     1561 ctcaagtaag agaaatagct gaattaaaga tgcctgactt aaatgctgca tcagtagaag
     1621 ctgctatgag tatgatagct ggtactgcta gaagcatggg tgttgtaatc gaagactaat
     1681 taaaagatac actttttttt ataaagtggg aggcgagaag ccgaaattaa ccacaaggag
     1741 gaaaataaaa tggctaaaaa aggtaaaaga tatgcaggtg cattacaaaa agtagataga
     1801 actaaatttt atgatgcatc agaagcatta acattagttt cagatatagc tggagctaaa
     1861 tttgatgaga cagtagaagc acacattaaa ttaggtgttg actcaagaca tgctgaccaa
     1921 caagtaagag gtgcagttgt attacctcat ggaactggta aaactaaaag agttttagtt
     1981 tttgctaaag gtgaaaaagc taaagaagct gaacaagctg gtgctgactt tgtaggagct
     2041 gaagaattag ttcaaaaaat acaaggtgaa aactggtttg actttgatat agtagttgct
     2101 actccagaca tgatgggtgt agtaggtaga ttaggtagag tattaggacc taaaggttta
     2161 atgccaaacc ctaaatcagg aacagttaca tttgatgtag ctaaagctat agatgaaata
     2221 aaagctggta aagttgaata cagattagac aaaactaaca taatacacgt tccagtagga
     2281 aaagtatcat ttggtggaga aaaattaact gaaaacttta ctgcattaat ggatgctata
     2341 ataaaagcta aaccagctgc tgctaaaggg caatatttaa gaagtataac agtagcttct
     2401 tcaatgggac ctggagttaa aataaaccca gcaagaacag ctgaataata aaactgttga
     2461 cacaggttga caaaaatgtt atcatatcac ttgtcaatgt aaaaaagaat agatttgccg
     2521 tagatagtgg gtgcgaaagc ttaatatccc gccgaggttt tagataaatt gagattacta
     2581 aagcttttga tgtctacttt tggcaacaaa agctttttat aatgttgcgg taaatactaa
     2641 gcctatttgg tgaaggaggt gcacatctat atgagaaaag ctatagaaat taaatcagaa
     2701 gttgtttctg agatagttga aaagttacaa aaatcttctg ctgctgttgt tgtagattac
     2761 aaaggattaa cagttgaaga agtaactgag ttaagaaaac aaatgagaga agctggtgta
     2821 gattacaaag tatataaaaa tactttagtt agaagagcag ctaaagaagt tggtatagag
     2881 caatttaatg atgaattatt agttggaact aatgccatag catttggata tgatgatcca
     2941 gttgctccag ctagaatatt aaaaggattc atggattctc atccaaaaat gaaattaaag
     3001 atgggtattg tggaaggtgc attttatgat gagtctaaga ttgttgaaat ggctaacata
     3061 ccatcaagag aagttcttat cgctaaatta cttggtagct taaaagctcc agtatcaaac
     3121 ttcgcatact taatagatgc aatagcgaaa aaggcagaag gtcaagaaga agcttaattg
     3181 cttaactaaa aaattataaa tattaatttg aaataaaatt cggaggtgct aaaaatgaca
     3241 atagaacaaa tattagaagc tatagaaaat atgaaagttt tagaattaaa tgaattagtt
     3301 aaagctgctg aagaaaaatt cggtgtatca gcttcagctc cagtaatggt agctggtgca
     3361 gctgctggtg gaccagctgc tgaagaaaaa actgagtttg atgtagtatt aactgacgtt
     3421 ggttcttcaa aagttggagt tataaaagca gttagagaaa taacaggatt aggattaaaa
     3481 gaagctaaag aagtagttga caatgctcct aagacagtta aagaaggagc ttctaaagaa
     3541 gaagctgatc aaataaaaga aaaattagaa gctgctggag ctaaagtaga agttaagtag
     3601 tttctttaaa aagataccca ttgggtatct tttttattta taaaaaatta taaaggaagt
     3661 atttttataa aataaaaaaa aattatataa tagtac
//
LOCUS       DAMJVM010000166         3679 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.171, whole genome shotgun sequence.
ACCESSION   DAMJVM010000166 DAMJVM010000000
VERSION     DAMJVM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.171"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            187..1767
                     /locus_tag="PC523_003406"
     CDS             187..1767
                     /locus_tag="PC523_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDF2476888.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTCGKEVNVFLVGNIEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYETSALIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRREKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     SKLLSSSDAIGFGPGMGNNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNVVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDELSKTMYTVN
                     ATDLISNISLTMKELLDI"
     gene            1937..2383
                     /locus_tag="PC523_003407"
     CDS             1937..2383
                     /locus_tag="PC523_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDF2476889.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVVLIFLSSFIESI
                     FLQILIFAVISIAMLVIATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVNATTSVTNQ"
     gene            2404..3447
                     /locus_tag="PC523_003408"
     CDS             2404..3447
                     /locus_tag="PC523_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDF2476890.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHLLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQIATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEIFD
                     VKEALGDNESLKK"
BASE COUNT         1479 a          333 c          698 g         1169 t
ORIGIN      
        1 gcttaattta tatggtataa taaattaaat aattacttta attcaaaatt ttttgttatt
       61 ttaaattatt tcaaaaatat tagttttagt gattttattt atgatattta tgtgcttaga
      121 aagatttcaa tagatatttt tttatgaata tttaagaagt ctttaacttt ataggagtga
      181 taatttatgc taataggtac atctaagtta agtagaaatg ttgatactgc atgtgtagaa
      241 gaagtaggta ttccaatgat tgtattgatg gaaaatgcag ttatatctgc aatgaaaaat
      301 atggatatag acatgtataa tagttataca gttgtatgtg gtgttggaaa taatggagga
      361 gatggtcttg gaatagcaag acatctaaat acatgtggaa aagaagtaaa cgtattttta
      421 gtaggtaata ttgaaaaact aagtgaatgt tctaaaataa attacaatgt acttttaaat
      481 atgggaatta atattataaa tattaataaa aatgatttaa gtgaaaaaaa ttatggagat
      541 gaaaatgaag tagataaaat tattaaagtt gagaaacaaa atattataaa aaataaaaat
      601 ttaacaattt ttaaagatag agtatctaaa agtgatgttg tagtagatgc tatatttggg
      661 actggactaa aacgtgatat aactggtatt tttaaagaag ttattgacat agtaaacgat
      721 aatagcaaaa atacatattc tatagatatt ccttcaggta tagatagcga taatggaaat
      781 gtattaggaa cttgtattag agcaaacaaa acgattagct ttgagtttta taaaagaggt
      841 tttctaaact atgagactag cgcacttata ggtgatgtag ttgtagaaaa gataggtgtt
      901 ccagatttta ttacaagaaa ataccatgat gaagagttca taactgataa gacttttgta
      961 aaaaataata ttagaagaag agagaagcat ggttttaaaa gtgattttgg aaaagtatct
     1021 atagttgcag gttcaaaagg attttatggt gcaagtttca tagcaactga atctgctgtt
     1081 aagagtggaa gtggacttgt aactttaatt agtagtgaag atgtgcaaga aaaggtatct
     1141 attagactta cagaagcaat gactttaaat tttgaagagg aaagattaag taaattactt
     1201 agttctagtg atgcaattgg ttttggtcca ggaatgggaa acaatagtca aacatttgat
     1261 aaattactta agatagtcga aaattctaat tgcccgatag tattagatgc tgatggattg
     1321 aacgttatga aggacagatg ttataagttt ttagaatgga aaaatagatt tgtaattaca
     1381 cctcatttgg gagaaatggc caggttgaca ggagattcaa ttgggtatat aagagaacat
     1441 agagtagatg ttgcaaaaga atttgctcaa aaatataatg tggttgtgct tttaaaaggt
     1501 tatcaaactg tcataacaga tggtaaaaaa acatatataa atcctacagg aaatagctgt
     1561 atggcaactg gtggaatggg ggattgtttg cttggaatga ttacttcatt tataggtcaa
     1621 ggtatggata tattggaagc tacagttagt ggagcatata tacatggcta tataggagat
     1681 gaattgagta aaacaatgta tacagtaaat gctacggatt taataagtaa catttcatta
     1741 acaatgaaag agttgttgga tatataatta atgatatttg atactacttt gtaacaactt
     1801 taacaaagta tgttgatata atactattaa ttaatttatt ttattgtttt gtaatttaaa
     1861 tttatattca aaaacattgt ataaacatat aatttatata gggttttata attcatatga
     1921 aaagagggta gatagtatga agttaatatg gttgatagta gcagtcttat ttggaatagc
     1981 agaaatgttg acaccaagct taacattgat atggtttagt gtaggagctg tagtacttat
     2041 atttttaagt agttttatag aaagtatttt cttacaaatt ttaatatttg cagtaatttc
     2101 tatagcaatg ttggtcatag ctactaaaaa aatagtaaaa aaagataaag gctacaaatc
     2161 taacactaat ttacaagcaa tgatgtctaa aaaaggtatt gttacagaag agatttctcc
     2221 aaataataca ggtctagttg tagttgaaca tgaaacatgg acggctattt ctatagatgg
     2281 tgaaaagata gaaaaaggtt ctacagtaga agtattaaaa attgaagggg taaaactagt
     2341 tgttaaaaaa gtaaacgcta caactagtgt gacaaatcaa tagaataaag gaggatttat
     2401 agtatgggta ctaaaattgt tttaagtatt gtattaatag tagtagtcgt agcaataagt
     2461 ttaacctgta taagagttat aaaacaatct aaagtaggta taataatgag acttggtaag
     2521 tttcaaaaag tggctgaaac aggtgtacat ttattaatac catttttaga taaaatggca
     2581 tatgtaattg accttagaga aattgtaata gattttccac ctcaaccagt tataactaaa
     2641 gataatgtaa ctatgcagat tgatacagtt gtatattata aagttacaga cccagttaga
     2701 tatgtgtttg agatagctaa tccaatagct gctattgaaa atttaacagc tacaacacta
     2761 agaaatataa ttggtgaact tgatttagat gaaacattaa catcaagaga tataataaat
     2821 gtaaaaatga gaactatcct tgatgaagca acagataaat ggggtataaa agtaaataga
     2881 gtagagttaa aaaatataat gcctcctcag gatattcaag ttgcgatgga aaaacaaatg
     2941 agagcagaaa gagaaagaag agaggcaata cttcaagcag aaggtaataa gtcagctgca
     3001 atacttcaag cagaaggtga aaaacaatct gctatattaa cagcagaagc taaaaaagaa
     3061 gctatggtac gtgtagcaga aggtgaaaaa gagtctgcta tattagtggc agaaggtgag
     3121 gctgaagcta taagacagac agctattgct aaggcacaag gtgaagctga aatgataaaa
     3181 agaactcaga tagcaacagc agaaggttta aaactagtct tttcagcaat gaaagaggct
     3241 gatatagata ataatattct agcattaaaa tctatggaag ctcttgaaaa aatggctgaa
     3301 ggtaagtcaa caaaactggt tttaccttca gaagcagtta atttcttagg aacattcaaa
     3361 ggaataaaag aagttatgag tgatgataac aaagaaatat ttgacgtaaa agaagcttta
     3421 ggtgataatg aatcattaaa aaaataaaag agttttgtta tcataggaag gatatggaaa
     3481 ttaaaggtcg aattaattaa atatagtaaa aaaatgcacg ttgaaaagaa gaggacttat
     3541 aaatttttaa gtcttcttct ttgtttttat tataaagatg aaattataaa gatgaattaa
     3601 cagaattttt atacaattta ttctttacta atgtaaaaag tgttatataa tattatataa
     3661 aacatatagc aaataagca
//
LOCUS       DAMJVM010000167         3519 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.172, whole genome shotgun sequence.
ACCESSION   DAMJVM010000167 DAMJVM010000000
VERSION     DAMJVM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.172"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            37..1119
                     /locus_tag="PC523_003409"
     CDS             37..1119
                     /locus_tag="PC523_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2476891.1"
                     /translation="MKKYMNKFKLKRNILISVIVLIFIFIIYFVSIELKDRSLVNNQY
                     KGKIKSANLSVDYEISQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFYTWQNTILNEIIIDVANPL
                     DVNVLNIGSNFVHLEGYQQNWSEIIDFVQSKFDGLVTYRTNWWYTKKDDLNSKLSYEQ
                     KLNNNFFDKLDFISIAAYFELSNKRINTVDELISALYSSTVNNRRQNIKKEIYNLYKA
                     HRKPIFFGELGFSNREYASSQPWNHATSKVVNGEEQARCFEAYKKVFENEEWINGFSV
                     FCIGKIDDEKNFYPSKESIKVIKSWY"
     gene            1176..2300
                     /gene="wecB"
                     /locus_tag="PC523_003410"
     CDS             1176..2300
                     /gene="wecB"
                     /locus_tag="PC523_003410"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDF2476892.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRANIKSIVCVTAQHRE
                     MLDQVIETFNINIDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIVLVHGDTTTT
                     LATSLTAFYNKIIVGHIEAGLRTYDKYSPFPEELNRQLTGVIADMHFAPTNLAKKNLL
                     SEGKPYNNIFVTGNTAIDALKMTIKENYSHPIIDDIGNDRMILLTSHRRENLGKPMKN
                     IFKAIKRIVDDFEDVQVVYPIHLNPKIRTIADEIFGKFPKKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLREKTERTEGIEANTLKLVGTNEDRIYNSVSNL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            2408..3382
                     /locus_tag="PC523_003411"
     CDS             2408..3382
                     /locus_tag="PC523_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF2476893.1"
                     /translation="MARKLIKNLGKSKSAKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLVDVIRSYNLQLIDNKVETK"
BASE COUNT         1472 a          336 c          606 g         1105 t
ORIGIN      
        1 tattataaat ttattatttt aaaagaggta tataatttga agaaatatat gaataagttt
       61 aaattaaaaa gaaatatttt aatctcagta atagtattaa tttttatatt tataatttac
      121 tttgtaagta ttgagttaaa agataggagt ctagtaaata atcaatataa aggtaagatt
      181 aaatcagcca atttatctgt agattatgaa ataagtcaag ttatgaagga tatagataaa
      241 ttaggattga atacagtaaa tgtacctata attataaatg tagagtccat taattctgat
      301 attatgtcaa tagataataa tagtaaagaa aaggcaatta agctaataaa aaaattaaat
      361 aaaaaaggta tttcaacaat attagaggca tatccatgga ttgaaaatgg aaaattgtat
      421 gaaactgact ggaaccctat aaataaaaaa agattttttt atacttggca aaatactata
      481 ttaaatgaaa ttataattga tgtagcaaat ccactggacg taaatgtttt aaatattggt
      541 tctaattttg tacatttgga agggtatcaa caaaattgga gcgaaataat agattttgta
      601 caatccaaat ttgatggtct tgtaacgtac agaactaatt ggtggtatac aaaaaaagat
      661 gatttaaata gtaagctgtc ttatgagcaa aaactgaata ataatttttt tgataagtta
      721 gattttatat ctatagcagc atattttgag ttaagcaata aacgtattaa tacagtggat
      781 gaattaattt ctgccttgta ctcatctact gtaaacaata gaagacaaaa tataaaaaaa
      841 gaaatatata acctttataa ggctcataga aaacccatat tttttggaga attaggtttt
      901 tcaaatagag aatatgcaag ttctcaaccg tggaatcatg ctacttcaaa agttgtaaat
      961 ggcgaagaac aagcaaggtg ttttgaggca tataagaaag tttttgaaaa tgaagaatgg
     1021 ataaatggtt tttctgtgtt ttgtataggt aaaatagatg acgaaaaaaa tttttaccca
     1081 tcaaaagaga gtataaaagt tattaagtct tggtactaat aattttgaaa gtttagaatt
     1141 tatagatgtt aaatagatga ttaggagaga tataaatgaa tgacatcaag gttatgactg
     1201 tatttggaac tagacctgaa gctatcaaag tagcaccttt aataaaggaa cttgaaaaaa
     1261 gagcaaatat aaaaagtatt gtgtgtgtta cagctcaaca tagagaaatg cttgaccaag
     1321 ttatagaaac ctttaatata aatatagatt atgatttgga tataatggaa aaaggacaaa
     1381 gtctgaatga tataacatgt aagattttaa ataaacttcc cttgatttta aacaaagaaa
     1441 atccaaatat agtattagtg catggagaca caaccacaac tctggctaca agcttgactg
     1501 cattttataa taaaattatt gtcggtcata tagaagctgg tcttagaaca tatgataaat
     1561 attctccatt tccagaagag ttaaataggc aacttacagg tgttattgct gatatgcact
     1621 ttgctcctac taatttggct aaaaaaaact tgctttctga aggaaagccc tataataata
     1681 tatttgtaac tggtaatact gctattgatg ctttaaagat gacaataaaa gaaaattata
     1741 gtcaccctat aattgatgat attggcaatg ataggatgat tttgttaacc tctcacagaa
     1801 gagaaaattt gggtaagcct atgaaaaata tattcaaagc tataaagcga atagtagatg
     1861 attttgaaga tgtacaggta gtatatccca tccatttaaa tcctaaaata cgaactatag
     1921 ctgatgagat atttgggaaa tttcctaaaa aaattcatat tattgaacct ttagatgtag
     1981 ctgactttca taacttttta aataaatctt atatgataat gactgatagt ggaggaatac
     2041 aagaagaagc tccgtcatta ggtaagccag ttcttgtgtt gcgtgaaaaa acagaaagaa
     2101 cagaaggaat tgaagcaaat acacttaaat tagtaggtac taatgaagat agaatatata
     2161 attcagtgag taatttatta ataaataaag ataactatgt tcagatgagt aaagcatcca
     2221 atccatatgg ggatggaaat gcaagcaaat atatagttga cataataatt aaaaaattta
     2281 attgtaagta tttaaattag gtttttatgc tcaacttgaa atttatatta gataagaatt
     2341 aaaacattaa cgaattgata taagatttat tatactatga gggatacaat ttttaagggg
     2401 gaataatatg gctagaaaat taataaaaaa tttaggtaaa agtaaaagtg caaagagagt
     2461 gaagctttta tttaagaaga tttttattac tgtattcata gtagcaagta tagttgctat
     2521 ttttaatata acgaaatatt ttgaggaatt atacaaagta agagatttaa aaagtactaa
     2581 aattgaatac tatatggatg tagctgatga agcaggggat ggaaaggttc agttaagttg
     2641 gaaggcctta cttgctatag acatggtaat acatgatgaa gatttaagta atataaaaaa
     2701 gaaagacaca ttggatatag gagaaaaatt tatagtagaa gataaaaatg ataaaggcga
     2761 aaaggtgtat aaggtaaaaa agtttaataa ggtattaagc gaattgaaat ttgactcttc
     2821 tcaaaaaagt agagcaagaa aatacatgaa ggatttggaa tacacatacc ttggaaataa
     2881 acaattagat agtagtgatg aaaaaattaa atttataaag aaattagaag attcagctat
     2941 aagagaatat attgattatg gaatattgcc ttctataaca attggacaag ctatattaga
     3001 atctggctgg ggaaattcta aacttacaaa acagagtaat aatttatttg gtataaaagc
     3061 agataaagca tggaaaggaa aaagtgtaga aatttcaact tcagagcatt ataatgaaaa
     3121 aattgtagct agttttaggt cttataattc attacaagat tctgtcaaag accacagttt
     3181 atttttaatt aataataaaa gatatagaaa acatggattg tttgaggcaa aagattatat
     3241 tagtcaagct caagcattag aaaatgcagg gtatagtaca gcagaagata aaaaaggaaa
     3301 tcgtatatat gcagagctat tagtagatgt tattaggagt tataatttgc aactgataga
     3361 caacaaagtt gagacaaaat aattgttaac tctatatgaa gagatggcaa gatgagatta
     3421 ttttctacaa aagaatagaa aaattgtaaa aaaaactaaa aaaatgtatt gacttgttat
     3481 ttaaaaggtg atattattat tactgtactc gaaagagaa
//
LOCUS       DAMJVM010000168         3494 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.173, whole genome shotgun sequence.
ACCESSION   DAMJVM010000168 DAMJVM010000000
VERSION     DAMJVM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.173"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            340..564
                     /locus_tag="PC523_003412"
     CDS             340..564
                     /locus_tag="PC523_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476894.1"
                     /translation="MEYSKGFKVIYSLVEYSKRFKAIYSVMEYSKGFKAIYDVMEYSK
                     GFKAIYDVVKYIQGDLNQSIVLLNIQRDLK"
     gene            737..1006
                     /locus_tag="PC523_003413"
     CDS             737..1006
                     /locus_tag="PC523_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2476895.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNTGKDQGFDPMVNIEQCRKWLEETVSN"
     gene            complement(1199..1444)
                     /locus_tag="PC523_003414"
     CDS             complement(1199..1444)
                     /locus_tag="PC523_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HDF2476896.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFVSTENPEPEKLKWWYWKD"
     gene            1862..2047
                     /locus_tag="PC523_003415"
     CDS             1862..2047
                     /locus_tag="PC523_003415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476897.1"
                     /translation="MFSKKNEGNEFSNEGKGISKKIIKFFKSTKGIALLAFIFGVFFG
                     NISSPACSEDHEEVISN"
     gene            2099..2380
                     /locus_tag="PC523_003416"
     CDS             2099..2380
                     /locus_tag="PC523_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2476898.1"
                     /translation="MSDAEPWFKMKDDEKKAIEAENQRKAEEAKKAEEEKKGYDTGIT
                     YDQLARTSDDYKYKKVKFEGKVIQVIEDGDEVQIRFAVSGNYDKVVLCS"
     gene            complement(2888..>3494)
                     /locus_tag="PC523_003417"
     CDS             complement(2888..>3494)
                     /locus_tag="PC523_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HDF2476899.1"
                     /translation="KHFYFNTDGIMQIGVFKGPDGFEYFAPANTDANNIEGQAIRYQN
                     RFLYLHDNIYYFGNNSKAATGWVTIDGRRYYFEPNTAIGANGYKIIDNKNFYFRNGLP
                     QIGVFKGPNGFEYFAPANTDANNIDGQAIRYQNRFLHLLGNIYYFGNNSKAVTGWQTI
                     NGNMYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
BASE COUNT         1282 a          411 c          495 g         1306 t
ORIGIN      
        1 aagtaaaaaa tatataataa tgttattatt taaaattatt atatattata ttaaaactta
       61 atgcaaaaac aatttttata ttatataatg aaacttaatt aaatataact aaaaaatcat
      121 taaatatatt aaataaatat ttaactttaa tgacctatat ttaattatgg ctataacata
      181 gaattatcaa atatattgaa taaatatttg acccttgaaa tgtgtaatta aattaagttt
      241 ggttatttga tggaatgcaa atttgatata aaaactccat ttaaagtgcc agataaaaac
      301 tttatggtta tataaaagct aaaagtaatt tatagtgtta tggaatattc aaagggattt
      361 aaagtaatct atagtcttgt ggaatattca aagagattta aagcaatcta tagtgttatg
      421 gaatattcaa agggatttaa agcaatctat gatgttatgg aatattcaaa gggatttaaa
      481 gcaatctatg atgttgtgaa atatattcaa ggggatttaa atcaatctat agttttacta
      541 aatattcaaa gggatttgaa gtaatttatt gtgttttgaa atattcaaaa atatttttgg
      601 aatagttttt ataaatttac cagtactaat aattttatgt ttaacgttta gatgacttaa
      661 aatcaattat ttgcactttt gtagtcatct aaagttattt tataatattt ttaaataatg
      721 gaaaggatgt ttgaatatgt ttgaatcaag atgtggtgtc tgttgtgaca gttgtacaaa
      781 aaaagaacaa gtaaattgta ctggttgccc aactatggaa aaaccatttt ggggtggaga
      841 atgtaaggtt aaaacttgct gtgaaagtaa ggaacttaat cattgtggtg aatgtgatac
      901 tttcccttgt gatatgcttt taaatacggg aaaagaccaa ggttttgacc ctatggtaaa
      961 tattgagcaa tgcagaaaat ggcttgaaga aacagtttct aattaattgt tttagaatgt
     1021 tttgttaagc aatttattta atactttgtt agatagtatg tttagtcatt tgttgattgg
     1081 tatatttagc catacaaata gatttacata aaatttacga ctaaaaaaga gttgagtatt
     1141 agtttttatt attaaaacag atattcaact cttttttata tctaactttt ttgatttttt
     1201 aatctttcca ataccaccac tttaattttt ctggttctgg attttcagtt gatacaaaat
     1261 atactgcaca tctgtataca tataactgac atctgtctat gtgttgtcca cgcattaaac
     1321 attccttttc atacatttgt gctgggtttg catctttaag tgatttaata gttgtatatc
     1381 ctaacatgat taaagatttt tctgttgctt tccctacccc tggaattttt tgtagttctc
     1441 ccatacttca ccttctttct ggtatattat ttttgtatta tacttagtac cagatatttt
     1501 tttattatag ttaatattta attttttatt atatcaattt atttatgctc tttcatctat
     1561 ctatatttta ccacctctaa agtactgagt catttaatta catcataata tagttttata
     1621 caaataaaat actttatgtt tcatttaata tataaaattc accttcaaga aaattatatt
     1681 ataatctgac atttttacct tattttccaa aatatattga attttcttgc tttattttgt
     1741 aaaattatgc ttaggggaaa tatattttag gaaaatatga atatataatt tttagtcaac
     1801 tagttatttt aagtttttaa attttaaaat aaaatatatc taataaaagg gaaattgtat
     1861 tatgttttct aaaaaaaatg agggtaacga atttagtaat gaaggaaaag gaatctctaa
     1921 gaaaataatt aaattcttta agagcacaaa gggtattgct ctactggcat ttatttttgg
     1981 tgtgtttttt ggcaatatat cctcaccagc ttgttctgaa gaccatgagg aggtcatttc
     2041 taattaaaca tcagttatag attctcaaaa aacagaaata gaaactttaa atagcaaatt
     2101 gtctgatgct gaaccatggt tcaaaatgaa agacgacgaa aagaaagcta ttgaagctga
     2161 aaatcaacgt aaagctgaag aagctaaaaa ggctgaagaa gagaagaaag gatatgatac
     2221 tggtattact tatgaccaat tagctagaac atctgatgat tataagtaca aaaaggtaaa
     2281 atttgaaggt aaggttattc aagttattga agatggtgat gaggtgcaaa taagattcgc
     2341 tgtgtctgga aattatgata aggttgtact atgtagttaa aaaaaatcaa taactccttc
     2401 aagagtgtta gaggatgatt acataactat aagaggtata agtgctggaa ctataactta
     2461 tgaatcaact atgggtggaa acataactat accaggaata gctgtagaga aaattaatta
     2521 atattgtttt gtattatagt taatattttt gtattatagt caatatgttt aaagatgttt
     2581 ttataattgc aaataaacag ttacaagact ctaaattagt ttttgctttt agcatattat
     2641 ctatgttcta gtaactatta attatttagt attaatattt ccatatatga attttattat
     2701 aaagtagtaa aattatttgt ttaaaattta gattatcgtc aagaataata gattattaaa
     2761 tgatagaaaa attttaacta aaagtcatgt attacaataa cacatgactt ttaattaaat
     2821 atcaatattt attatataaa aataatttct gagtatcaca tgaataaatt tttgtcaaac
     2881 atatattcta gccatatatt ccaggggctt ttactccatc aacaccaaag aaatatataa
     2941 caccatcaat ctcgaaaagt ccaccagctg cagccatagc agtatcaggc ataaagtaat
     3001 acatattacc attaatagtt tgccatccag taactgcttt tgaattatta ccaaagtaat
     3061 atatattgcc aagtaaatgt aggaatctat tttgataacg tatagcttga ccgtctatat
     3121 tgttagcatc cgtattagca ggtgcaaagt attcaaatcc attaggtcct ttaaacactc
     3181 ctatctgagg taaaccattt ctaaagtaaa agtttttatt atcaataatt ttataaccat
     3241 tcgcacctat agctgtatta ggctcgaagt aatatctcct accatcaata gttacccaac
     3301 cagtagctgc ttttgaatta ttaccaaagt aatatatatt gtcatgtaaa tataggaatc
     3361 tattttgata acgtatagct tgaccttcta tgttgttagc atccgtatta gcaggtgcaa
     3421 agtattcaaa tccatcaggt cctttaaaca ctcctatctg cataatacca tcagtattaa
     3481 aataaaaatg ttta
//
LOCUS       DAMJVM010000169         3450 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.174, whole genome shotgun sequence.
ACCESSION   DAMJVM010000169 DAMJVM010000000
VERSION     DAMJVM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.174"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(57..629)
                     /locus_tag="PC523_003418"
     CDS             complement(57..629)
                     /locus_tag="PC523_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2476900.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(658..924)
                     /locus_tag="PC523_003419"
     CDS             complement(658..924)
                     /locus_tag="PC523_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HDF2476901.1"
                     /translation="MKDSSRAELMRKIQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            complement(924..1133)
                     /locus_tag="PC523_003420"
     CDS             complement(924..1133)
                     /locus_tag="PC523_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HDF2476902.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            1402..2262
                     /locus_tag="PC523_003421"
     CDS             1402..2262
                     /locus_tag="PC523_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDF2476903.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2310..3338
                     /locus_tag="PC523_003422"
     CDS             2310..3338
                     /locus_tag="PC523_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2476904.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRNLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNIDIQNLLIIRDIIRAPFNDINSNLDNCFDKF
                     KESLYKKEENYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEVMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDILEDLMLYRMHEDEYKHEIENIIKMLDEDLDNEKIYINKCN
                     DLNVVNKI"
BASE COUNT         1355 a          377 c          559 g         1159 t
ORIGIN      
        1 gacacaacag taaattataa attatcaaat aatagtagtt tatataaata aaaattttag
       61 aacatttttt gagattcttc taactcgtaa gcgtccatta aagcttcgcc aaatctttga
      121 tagtgaatta tttctctttg acctaagaat tttagtacat ctataacatc aacatcatct
      181 gttaggttta taagttgata ataagttgca agtgcttttt gttcagctgc catatcctca
      241 tgtaaatcag ttacggggtt tgacataact gctataggtc ttacatcaaa tgggacacca
      301 tttgaatctg ttggataaat tccatatcca ttttgtgcat agtttgaacc aagacctgca
      361 gcttttaact cttctgggct tgcatcagaa gtcaactgat aaaccatagt acatataagc
      421 tcaacgtgag ctagctcttc tgtaccaata tctgttaaaa gtgcacgcat attaccagta
      481 ggcattgtgt atctttggct taaatatctt aaagatgcag caagttctcc atttggtcca
      541 ccaaattgag ttataagaaa tttagccatt ctagggtcac aagtttttat attaactggg
      601 tgttgtatag ttttctgata tatccacatg aattttcaat cctccaatac ttgatgtcta
      661 ataattaaat tctctgtccc aaggccaagg ttggtcagcc cattgccaag ggcatctact
      721 tggagcatac ccaaagttag ttaaaggacc atatttattt tcgtaagcat atctagcttg
      781 cgcaaattga ttacataatg aattataagt gttgatagca tttttatcat cagggtgatt
      841 gtctaaatat aagttcatat caacacatgc aaaagatgtt tcctgaattt ttctcataag
      901 ttcagctcta gatgagtctt tcattatctt ctacctcctc tatatttttt tggcttagca
      961 tataattctt tgttgtacat ttcagattct agtaagttta gttctggaaa tatagttcca
     1021 aattttaatg catcagaaag gcagtacatt tctgtaaaaa tttgattatt aatgtatggt
     1081 ctagccaaag atggaccaca attttttttg atatctctag tcttattttc caagatgaaa
     1141 acctccttgt tgtaaataga taggtaactc tatctagtgt ataatattca atcgtttaaa
     1201 aatcgttact aataataaaa tattaaatga gaaaaataaa aaagataata aaagaaagag
     1261 attatttatg ttattataag tttatgtgtt attgtaaact ataaattatt tagtaaaatt
     1321 aaagtaaaaa ttatggtatt ttgtggatga aggtagactt ttttgtaaaa tattattaac
     1381 ataaatatat gaggtgatta aatgagtgag ctatcaggaa aaggctgtga aattatagtt
     1441 ccttttgagg aaagacttcc agtaagagat atagaaaaaa gtataataaa aaaatataga
     1501 aaaaatttat ggtccaagtt tatgaaggca ataagagatt ataaattggt tgaagaaggg
     1561 gataagatag ctgtagcaat ttctggtgga aaagacagta ttcttatggc taaaatgttt
     1621 caagagttaa aaaaacatgg acaagttaat tttgatgttg aatttattgc tatggatcct
     1681 ggataccatg caaatataag acaattactt atagataatt gtgagtatct aaatatacca
     1741 atacatttat ttgattcaag aatatttgag atagcagatg aaatagcaaa agattatcca
     1801 tgctatatgt gtgcaagaat gaggagagga gcattatatt caaaagcaga agagttaggg
     1861 tgcaataaac ttgcattagg tcatcattat gatgatgtaa tagagacaac aatgcttaat
     1921 ttattgtgtg ctggaaactt taaaacaatg cttcctaaac tcaattctac taattttgaa
     1981 ggtataaaaa ttataagacc actttattat ataagagaag aacatattat aagatttatt
     2041 caaaatagtg ggatatggcc attaaattgt gcatgtatgg ttgctgctaa aaaaacaggc
     2101 aataagagat atgagataaa agatttaata aagagtttag aatcaaattt taaaaatgta
     2161 gaaaagtcta tatttaaggc agcagaaaat gtaaatcttg actcagtact tggttggcaa
     2221 aaagatgggg agaaacattc tttcttagaa aattttgaat aaaaataatt aaaaagaatc
     2281 actataaaca attagaaagg gtgtttatat tgaaaagagt atatatagat tttgaaatga
     2341 atatgccaaa ttctaaatca aaaagagcag tatttaattc tgatataatt gcaatcggag
     2401 cagttatgta tgatgaaaaa acaaaaaata ttgataaatt taaatcttta ataagaccag
     2461 taagtaatga ggaattgtat cctcatatac aagaattaac acatatatct tctgaagaat
     2521 tgaagagtgc accaagttat gaagaggtca tgagaaagtt taaaaaatgg ctgggcatat
     2581 tttctgatat taaaggaata tatacttttg gtaatttaga tttgacatgt ttcaataaca
     2641 cagatatgag aagtgctaaa aaaaataatc atccaaggtt tgtaaacaat attagaaatc
     2701 tatttgtaga tataaaagaa aaatatataa attgtggtat gagatgtatg aactatatat
     2761 cattgaaaaa tttacttgaa tttgttaatt tagagtttag tggagatgct catgacccac
     2821 taaatgatgc atataattta tttattttgg acgaggcaat tacaaataat atagacatac
     2881 aaaatctttt gataatacgt gatataataa gagcaccatt taatgacata aattctaatt
     2941 tagataattg ttttgataaa tttaaggaat cactatataa aaaagaagaa aattacaata
     3001 ttgtagattt ttcagttgaa ataataaaaa ctgtgagaat gtacttatta acaataatag
     3061 atgtaaatat acaaaattta gaagttatga aagatataag taaaaaaatg gatactatag
     3121 ataaattgaa agatatagag gaaggctatt tttatttatt agaagatgta tattttgata
     3181 tgaaagatat attagaagat ttaatgcttt ataggatgca tgaagatgag tataagcatg
     3241 agatagaaaa tataataaaa atgcttgatg aagacttaga taatgaaaaa atatatataa
     3301 ataaatgtaa tgacttaaat gttgttaata agatataaga aattataaat aatagagtag
     3361 aacttgataa ttataacaat tttttataaa actgaaataa aattaaatta atataaaaat
     3421 aatatacaat aaaaaatgta gagagatgat
//
LOCUS       DAMJVM010000170         3380 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.175, whole genome shotgun sequence.
ACCESSION   DAMJVM010000170 DAMJVM010000000
VERSION     DAMJVM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.175"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            50..1159
                     /locus_tag="PC523_003423"
     CDS             50..1159
                     /locus_tag="PC523_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2476905.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKINRRIIEL"
     gene            complement(1382..1771)
                     /locus_tag="PC523_003424"
     CDS             complement(1382..1771)
                     /locus_tag="PC523_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5071 domain-containing protein"
                     /protein_id="HDF2476906.1"
                     /translation="MENIEELLPKDKFDNSNIEKLKLLSDEEIRPICYRLLEWMQDYN
                     WPVAKEILPVLALHQELITPLIIDVLTEEDDDWKYWIISCLLPLFADKNLEPIVPFVK
                     RIAENPTKWECEAEVDEIAKVFLKRIL"
     gene            complement(1947..3137)
                     /locus_tag="PC523_003425"
     CDS             complement(1947..3137)
                     /locus_tag="PC523_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476907.1"
                     /translation="MQKNVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNDCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGIVLLAFTIFGAMLVSKAATAMTIA
                     ILLCTLTIFIVGIKAKAPEITEILSNRTSFTPGYFKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMILSFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     IENL"
BASE COUNT         1251 a          512 c          438 g         1179 t
ORIGIN      
        1 attaattgat taataaaaaa gaaaattttt catgaaaggt aagataacaa tggatttcaa
       61 aagcttaggg attagtgaaa atacaataaa tattctaaaa aaatcaggaa taacaacacc
      121 tacacctata caaaaagaaa gtattaaact aataaaagaa ggaaaagatg taatagcaga
      181 agctcaaact ggaacaggaa aaacgctagc ttttttactt ccgatatttg aaaatatatc
      241 attagatata aatgatatac aagttcttat actttctcct acaagagaac tagctattca
      301 aataacagaa gaagctatga aacttaaaga gtctaaagat gtaagtatcc tagcagcata
      361 tggaggaaaa gatattggtt ctcaaataaa gaaactaaag ggaaatattc atatgattat
      421 agctacacca ggtagacttt tagaccatct aaatagaaaa acaatagacc ttagtaagtt
      481 aaagactttt gttttagatg aggcagacca aatgttgctt atgggattta aaaatgaagt
      541 ggaagccatt ttaaaagaaa cttcaaacaa aaagcaaaca ctatgctttt ctgcaactat
      601 aaattctcaa gtaaaaaaat tagcttatag gtacacaaaa aatcctgttg tggtttccat
      661 tcaaaaagaa gaaataactc ttaataatat aaaacaagaa gtagtagaaa ctacagatag
      721 aaaaaaatta gatgcattat gtaaggtgct agatgaagat aatcctttta tggcaataat
      781 attttgtagg actaagagaa gagttgataa tcttgaagaa gctcttgcta taagaggata
      841 taattgtcaa aaacttcata gtgatattgc tcaatcaaaa cgagaaagaa taatgaaatc
      901 atttagaaat ttagatatac aatacttaat agctacagat gtagcttctc gtggtcttga
      961 tatatctgga gtaagtcata tatataatta cgatttacca gaaacaccag aagactacat
     1021 acaccgtatt ggtagaactg gtagagctgg tgaagaaggc tatacatgtg cttttattga
     1081 ccctaaaaat gaacgaatgt taagtgaaat agaaacagct atagaatcta aaattaatag
     1141 aagaattata gaattataat ttatggacta attaaaatgc caatagtata taaatactat
     1201 aatatacaga aagcaggtat caactttttc tttattataa tactatttta tattgcataa
     1261 aaaagctgta acctttatga ttacagcttt ttctatctat tgttagtttt atattacctg
     1321 tttccaagtt gtttaatatt aaattttgaa aataatctca ataaatatat tcaactatta
     1381 tttataatat tctctttaaa aatacttttg ctatttcatc tacttctgct tcacattccc
     1441 atttagttgg attttcagcg attcttttaa caaatggaac tataggttca aggtttttat
     1501 ctgcaaacag tggcaaaaga catgaaatta tccaatattt ccagtcatca tcttcttctg
     1561 taagaacatc tattattaat ggagtaatta actcttgatg tagagctaaa acaggtaaaa
     1621 tttcttttgc aacaggccag ttataatctt gcatccattc caataatcta tagcaaatcg
     1681 gtcttatttc ttcatcagat aataatttta atttttctat attactatta tcaaatttgt
     1741 ctttagggag caattcttca atattttcca taacttaatt ctcctaataa atacattatg
     1801 ttcattatat ctttcaaatt ttaatttatc aatttatttt tcaaccctat tttatcatat
     1861 ctaaatagta atatactcta tttttatttg actatatttt cgacctatta attagattat
     1921 atcttttatt taaagtacta ctcactttat aaattttcaa tattttcgtt taattccgct
     1981 ggccatttag cctctggatt ttcctttgca aacttcttgt tcttataatg tcctaatgta
     2041 aggaatggaa ttactatagt aaataaacca agataaccac aatatccata tccatattta
     2101 acaatattac ttaatcctgt aagtgatata agcattgaca ctaccattat tgcacaagct
     2161 gtaaataccc ttcttttcat tcttgaagaa aggaaagaaa gtgctttatg gtcttcaaat
     2221 ctatttacaa gaccaaatat agtagttact cctgtagaca ttaaacaaag gaataatgca
     2281 acattataaa ttatgtatat agtatgattt ccagattgac ctgcaacata aagagtaggt
     2341 agtgttgttt gaccagcagc aataatttct ctataccatc ccattagcat agttacagca
     2401 agccctaatg caattgcatt cataacaaat gaaagtatca ttgccttagt agcttctttt
     2461 gagttcttaa gaagtggtct tgaacatccc gcaagtgtag gtataacaac tgattgaaaa
     2521 cctgcataaa taaaagtatt aagtattggc ttgaaatagc caggtgtaaa actcgttcta
     2581 tttgataata tttcagttat ttctggtgct ttagctttta taccaacaat aaaaatagta
     2641 agagtacata atagaatagc tatcgtcatt gctgtagctg ctttagatac aagcatagca
     2701 ccaaatatag taaaggcaag aagtacaatt ccaataataa atacagctac aaaataattt
     2761 acccctatac tttgaaatac tgctgctgca cctgctataa cagcacttac agctattaaa
     2821 accattagat agtaatatat ctcccatatt atttcaagtt ttggatatgg tctccaaagc
     2881 tcacaaaata aatccttata attcctacaa tcattattat tatacattat gataacttct
     2941 ctcatggtag cagtaagtat aatcattgct aaaattgcca taagtggtgc tgtccatcca
     3001 tactgcacat agtattgagt ttcttgattc cctgtagcaa atcctcctcc tgcatgtgaa
     3061 ctaaaaagaa cgcttgcaac gccaaaaatg gctaacatag agcccgttac ttttgctttc
     3121 tttacatttt tttgcatgaa atcatcctcc ctaaaatgat attaaatgtt tttattttaa
     3181 cacttcattg ataaaacaat aacatttttg ttcattaaat tcaactatat tttcccaata
     3241 tttttatatt ttcaactttt ttccctatct atattaaata ttttttatat atttatttta
     3301 ttttttaaga atgttcaaat ctacttcgtt ttttaatttt atgaaatatc ttttttaatg
     3361 ttaatttcta cagatttttt
//
LOCUS       DAMJVM010000171         3362 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.176, whole genome shotgun sequence.
ACCESSION   DAMJVM010000171 DAMJVM010000000
VERSION     DAMJVM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.176"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            36..932
                     /locus_tag="PC523_003426"
                     /pseudo
     CDS             36..932
                     /locus_tag="PC523_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            1334..3238
                     /locus_tag="PC523_003427"
     CDS             1334..3238
                     /locus_tag="PC523_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2476908.1"
                     /translation="MKIKFIIVVSIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LVSYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLLRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNDIPLVSANVYEQSGEN
                     YVNPYVMKNINTDKGVVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKI
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNIKEMTKWAKEW
                     QYNENKGIHKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEENLSSSSLSKNSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         1396 a          353 c          632 g          981 t
ORIGIN      
        1 caggaaccct gtgactttag tcatgggagg ttcagaagga agtaagtata gttgctactg
       61 cagatttaca ttcacatttt tctgataaat taacagtcga ccttgttaat gaacgaagca
      121 acccaattga acctgttgta gtagatgcag gtgatttctt agaccctcaa acagatgaaa
      181 tgactcaatg gtataaagag tggaaaaata taagagataa taatagtggt aaaacgatta
      241 gtagatgccc aatggtcatg gatatgaatc agggaatgta tgatgcagta gtacttggaa
      301 atcatgagtt tgtatctgaa aaagatgagt tttggtcaga tgagcgtctt ttagatgctg
      361 ttatagagga ttttgaacag gtgacaattt ctgtgttatc agcaaatata tataagcaat
      421 ctggtaaaaa ttatgttaaa ccatatatta taaaagatgt agagacgagt gaaggtaatg
      481 taaaagtagg aatattaggg cttactataa aagaagtagg taaaggtctt gaaaatgtaa
      541 atttacaaga gcaatttcaa tataagggga aattgtatgc aaatgattta gtagaagatg
      601 caaagaaatg ggtaaaaatt atgaaaaaga aaaaaccaga tataatagtt gcagttgtac
      661 attcaggaga agagcctaaa aatccaaaac atccaggaaa tagaataaag gagctagcaa
      721 ctacagtaga tggtattgat gcgattgttg ctagtcatac tcatgaaaaa atagatgaac
      781 atgaatataa aaataagtct ggagagaaag ttatagtgac acaaccaggt gagcatggta
      841 aatattattc taaaattact tttaaagtaa ataaagaaaa gggttcatgg attattaatg
      901 ataaattcag taaaacaatc gaggttgaat aactttataa aatctataga taaaataaat
      961 tatctgatat tgacataata tacaagataa agtttccaat attagtacag agcataaggt
     1021 aaaatattct aataataaga gaataaacaa gataaataaa aatatttcaa catataatat
     1081 atagtatgaa catattatac tactccttac tttataattt gaacatgtta gaataagtct
     1141 tacttgacaa ttttagtgta ttagagtaag tattattttg ccaataaaaa acattgagaa
     1201 ctatatgtag atttgcttaa aaaggaattt acatatagtt cttttcgtat aataaatagt
     1261 acaatcttat gtggtaaaga taacgcgtga tattaaattt tattatcatc caaaatctaa
     1321 ggaggagtct tttatgaaaa taaaatttat tatagtagta tcaataatat ttttaatagt
     1381 attacttgta gggtgtcaaa gtactaaaga tacagaaata aatgtactcg ctacaagtga
     1441 tttacattca attattccag ataatttagt ctcttatgta gaggaagaga gaaaatatga
     1501 tgagaatcta cttatggtag acgcaggaga tttttttgac atgcaaagtg gtgagatgaa
     1561 caagtggttt actggtcaaa aactattaag atttaaagat gggataccag agtacaaaaa
     1621 aatagccgaa ccacgtgaag gtgaagttcc attagctaaa aaaatggcaa agctgaaata
     1681 tgatgcagtt acatttggga atcatgaatt tgtatctaat gacaagcaga gccttgataa
     1741 attagtttct gattttaaaa ataacgatat accattagta tcagcaaacg tatatgaaca
     1801 atctggggaa aactatgtaa atccatatgt aatgaaaaat ataaatactg acaagggagt
     1861 tgtaaaggtt ggtatacttg gtttgactat aaaagaagta ggggaacgtt caaggtttaa
     1921 aagttttgaa caagataaaa aggctagaga acttggggag caacctcaat acaaagggaa
     1981 attatatgcg aatgatttgg tagaagatgc aaataagtgg gtaaaggtta tggaaaaaaa
     2041 agaaaagcca gatataatag ttgctattgt acattcaggt gaagagccaa aaaatcctaa
     2101 aaatccagga aatagaataa aagaacttgc aactactgta gaggggatag atgctattgt
     2161 agcaggtcat acacatgaaa aaatagaaca gcatacatat aaaaataatt ctggtgaaga
     2221 agtaatagta acacagcctg gagctcatgg taatagtata tcaaagatta attttaagtt
     2281 ggataagaag aatgataagt ggtttataaa agataaatat agtgaattaa aaatttttgg
     2341 aaaggaagta aatataatta caacctctgg tctgaatcca aaattttcag atgaaatggc
     2401 agttaatttg tttaatgaaa gagataggga tgttcaacct atatttatag atactggaga
     2461 ttttttaaat actaatataa aagaaatgac taaatgggct aaagagtggc aatacaatga
     2521 aaataaaggt atacataaaa aaataaatga atggccaatt gtatatcttg gttatgatgc
     2581 tgtagtactt ggtagtcatg aatttacaga agataaagag acttttgagg aaaaaaaatc
     2641 tactttggat tatattatag aaaactttga agaaatgaag ataccagtat tatcagctaa
     2701 tatatatgat gaatctggag aaaattatgt aaaaccttat ataattcata atgtagaaac
     2761 tagggaagga aatataaagg ttggagtatt aggtcttact actactgata aggatgaaga
     2821 aaacttaagt agctcaagct taagcaaaaa ctcaaagact tcgaaaattc ctgaagaaac
     2881 aggaaagtta tataccaaca atctagtaaa agatgcaagc gaatgggtaa aagttatgca
     2941 aaaggaaagt cctgatgtga ttgttgcagt tgtacattca gataataata gtactctaaa
     3001 aaatccaagt agtgaagtaa aaaaacttgc aatgtcagta gatggaatag atgctattgt
     3061 tgtaggtcat acgaaaaata aggtagaaga acatatttac aagaataagt ctggggatga
     3121 ggtcatagta acacaatctg gagaagattg ttattcaaaa ataagtcttg aactaagaaa
     3181 agaaaatggc aagtggaata tcataaacaa atatagtaaa gcaataaaaa tgaagtgaat
     3241 ttattgatat ataaaccatt attaacactg atttttataa tatttaacac tactttttat
     3301 actataatta acactgattc tataatatca ttaacattac ttttatacta taattaacac
     3361 tg
//
LOCUS       DAMJVM010000172         3186 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.177, whole genome shotgun sequence.
ACCESSION   DAMJVM010000172 DAMJVM010000000
VERSION     DAMJVM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.177"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     misc_binding    11..273
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            348..3014
                     /locus_tag="PC523_003428"
     CDS             348..3014
                     /locus_tag="PC523_003428"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDF2476909.1"
                     /translation="MENTNLSKTYNPKEFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPEKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDTNAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
BASE COUNT         1262 a          352 c          626 g          946 t
ORIGIN      
        1 atatgtaata ttaaagactg agaagaagag gagtaagtaa atactactaa aagagaggaa
       61 aattcactgg ctgtaagatt ttctaagtat aagatttact gaatgtagct ttggagtttc
      121 taaactgaac tatgagtaag tttagacggt tagaaccgtt aaattctttg agagtcaaag
      181 tttataaaat aatattaaca aataatttaa ttatacgtta atgtatttta agctttggaa
      241 ttaaggtggt aacgcgagct tttcgtcctt tttgaagagg atgaagagct cttttttatt
      301 tctaaagata tttaaaaatt tatttataaa atacaggagg caggacaatg gaaaatacga
      361 atttatcaaa aacttataac ccaaaagaat ttgaggctag gctatataaa aaatggatgg
      421 atgaagggta ttttaaatct aaaccaaatc cagataaaaa accatttaca ataatgatgc
      481 caccaccaaa tataacaggg caattgcata tgggacacgc tcttgaccat acacttcaag
      541 atatattaat tagatggaaa cgtatggatg gatatgaagc tttttggctt ccaggaactg
      601 accatgcttc tatagcaaca gaagttaaag ttgttgaaag aataaaaaaa caagaaggta
      661 agacaaagta tgaaataggt agagaagaat ttttaaagag agcctgggag tggaaagatg
      721 aatttggagg aaaaatatct aaccagctta aacaattagg tgattcttgc gattgggata
      781 aagaaagatt tactatggat gaagggtgta atgaagcagt tatagaattt tttgtaagcc
      841 tatatgaaaa aggtcacata tacagaggaa acagaataat aaactggtgt ccagattgta
      901 aaactacttt atcagatgca gaagttgaac atgaagaaca tgacggaaat ttttatcata
      961 taaagtatcc actaaaagat agtgaagact tcttagaaat agctacaact agacctgaga
     1021 caatgatagg tgatactggt atagcagtaa atccagagga tgatagatat aaacatttaa
     1081 taggaaaaac agcaatactt ccattggtag gaagagaact tccaatagtt gcagatagtt
     1141 atgttgattt agagtttgga actggtgcag ttaagatgac tccagcacat gaccctaatg
     1201 attttgaagt aggactaaga cataatcttg agcagttaaa tactatgaat gaagatggaa
     1261 ctatgaatga agtttgtgga aaatatgaag gtatggacag atttgagtgt agaaaagcta
     1321 tcgtagcaga cttaaaagaa caaggttatc taattaagat aaaagagcat aatcataatg
     1381 taggaacttg ttatagatgc catacagtag ttgagcctag attatcagag caatggtttg
     1441 ttaagatgga agaacttgca aaaccagcta tagatattct taaaaaagga gaattagaat
     1501 ttgttccaga aaaatttgat aaaacttatt tacaatggct agaaaatata agagattggt
     1561 gtatatcaag acaattatgg tggggtcatc aaataccagc ttattattgt caagagtgtg
     1621 gagaaattgt agttgctaga aaaatgccag ataagtgtcc taaatgtgga agcactcatt
     1681 ttaaacagga tgaagatgca ctagatactt ggttcagttc tgcattatgg cctttctcta
     1741 ctctagggtg gccaaataag actgaagctt tagattatta ttatccaaca agtgtacttg
     1801 ttacaggata cgatataata ttcttctggg tagttagaat ggcatttgca ggtatgttct
     1861 gtatgaatga aaagccattt gaccatgttt tggttcatgg attagtaaga gattctcagg
     1921 gtagaaaaat gagtaaatct ttaggaaatg gtatcgaccc attggagata atcgagcaat
     1981 atggagctga tgcactaaga tttacattaa caactggaaa ttccccagga aatgatatga
     2041 gattctatat ggaaagagta gaatttgcta gaaactttgc gaataagttg tggaatgcat
     2101 caagatttgt atttatgaat attgatgaag atataattaa aaacatgact agagaaagtg
     2161 ttaaagagga cttaactcta gctgataaat ggataatatc aagagcaaat aatattgtaa
     2221 aagaagcaac taataacatg gataagtttg acttaggaat agcacttcaa aagatatacg
     2281 attttacttg gtcagaatac tgtgattggt atatcgaaat ggttaaacct agattgtacg
     2341 gggaagatac aaacgctaaa tctgcagcat tatatacatt aacttatgtt cttgaaaaga
     2401 tattaaaatt attacaccca tatatgccat ttataacaga ggaaatatat actcatcttc
     2461 ctactgttga aggatgtata atagtatctg aatggccaaa atataatgaa gaagataaca
     2521 tggctgaaga agaagatatg atgaacttat tgatggaagg tataagaagt ataagaaatg
     2581 tgagagcaga aatgaatgta ccaccttcta aaaaagctaa attgataata atcccaagtg
     2641 aagaaaaaat agaagctatt gagcttggca aagattattt tataacttta gcatctgctt
     2701 caaatgtaga gatagcaaaa gataaatcta atgttccaga agatgcagtt ggagtagtta
     2761 ttgatggagt tgaaatattt atacctctta atgaattagt tgattttgaa aaagaaattg
     2821 agagattatc taaagaaaag aaaaagttag aaggagaaat aaagagagtt aatggaaagc
     2881 ttgcaaatca aggtttctta gcaaaagctc ctgaaagttt aattgaagaa gaaaaagcaa
     2941 aaaaagaaaa gtttgaagag atgattaaat ctgttgagga aagattaaca aatttagaat
     3001 ctaaaataaa ataaatataa gaagaaataa aacatacaaa aagtatatat tctatacttt
     3061 ttgtatgttt ttctttatat atatacaaat atgatgttat aattctttac agatgtgtta
     3121 tatttttatt ttctgttatg aatatatgaa ttttatatct taaaatttat aagcaataaa
     3181 aatttg
//
LOCUS       DAMJVM010000173         3172 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.178, whole genome shotgun sequence.
ACCESSION   DAMJVM010000173 DAMJVM010000000
VERSION     DAMJVM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.178"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            237..614
                     /locus_tag="PC523_003429"
     CDS             237..614
                     /locus_tag="PC523_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2476910.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDTTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIIVKHKDYLSFETKDFLSNIHDDSLKSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            607..1641
                     /locus_tag="PC523_003430"
     CDS             607..1641
                     /locus_tag="PC523_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HDF2476911.1"
                     /translation="MNNYMIYVFENLIKTTIVCSLGICLLLFLKRYVFKKFSKRFNYY
                     IWLVMVFRMLLFLFNYTVIYEVKEPKENTVGNNITQIDISTDNNLVLYLAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYSIEDNDVNCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSRDELSWILKHELMHFKNKDILIKFLVLSVKIIYWFNPLVYI
                     MSNKVNLDCELCCDESVLTDCSLKDKKEYALALIKSIKLSRNYNSGILTTEFNKTSLE
                     KRLDSIVKKKGKSGILVLLILFTLFSVTYFNFESISFDNSNSLRNIGILEKSGADLKE
                     ENDISMRIVN"
     gene            1707..2294
                     /locus_tag="PC523_003431"
     CDS             1707..2294
                     /locus_tag="PC523_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HDF2476912.1"
                     /translation="MKNLKKILIVTGCLTLISTSLVFANTTTDVDTSKDDDSVISSCT
                     ITGYEFTYENAPQSVKFDYEKKCEALNIKPSKDDIIFVNKDSIDVNDIENYVEPKAYI
                     IRYKKGYVFEVTGPKSYTLDVSDTVVGYNRVESGNPVHLVQLLCRTLDGSSIKDDSEF
                     GPATYNAVKSLQGRLGLTRDGIVGRSTWDAAGRRL"
     gene            complement(2510..2862)
                     /locus_tag="PC523_003432"
                     /pseudo
     CDS             complement(2510..2862)
                     /locus_tag="PC523_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1218 a          293 c          479 g         1182 t
ORIGIN      
        1 tattgaaatt tttgttaaat agttttaatg tgtaataaca ttttatacta ttgtaattaa
       61 atatgtaaaa aggaaaatat ttaaatagat atattttaaa atctaacttt taatattgta
      121 aaaaaattag aaaattaaca aaaaatacaa aaattcacat ttttttcata aagtgtttga
      181 ctaaaaaatt cacatgttgt agaattaagg tagcaagagt tttgagggag gtgtaaatga
      241 tgataagaaa aataccacaa gcagaactaa aagtaatgaa attcatttgg aaagtagata
      301 ctacagtgac atcaaaagat gttattgaag ctatggaaca aaaatatggc tggaaacaaa
      361 ctactacact gacgctttta tctaggctgg taaaaagagg gttcttagat gctcaaaaaa
      421 tagacagata tacacattat acaataatag taaaacataa agattattta agctttgaga
      481 caaaggattt cctaagtaat atacatgatg actctttgaa aagtttaatt tcagcattac
      541 atgatgatga aaatataggt aaagataagc tagatttttt agaaagttat tttgataatt
      601 taaaagatga ataattatat gatatatgtt tttgaaaatc ttataaagac aactattgta
      661 tgtagtcttg gtatttgtct attattattt ttgaaaagat atgtgtttaa gaagtttagt
      721 aagagattca attattatat ttggttagta atggtcttta gaatgttact ttttctattt
      781 aattatacag taatatatga agtaaaagaa cccaaagaaa atacagtagg aaataatatt
      841 acacaaattg atatttctac agataataat cttgtgctgt atttagctta tttatggtta
      901 tttgtaacta tagtcattgc agtttataca tttataaagt atacaagatt taaaaatctt
      961 gttgttgatg tatcttatag tattgaagat aatgatgtaa actgtatcta caaaaacttg
     1021 ttgaaagaat taaatataaa aaagaaaatt gagttaagag gttctgatga attgataagt
     1081 ccagcaggca tgggattatt taaatcttac atattcttac cagactatcc ttatagtaga
     1141 gatgaactaa gttggatttt gaagcatgaa ttaatgcatt ttaaaaataa agatatttta
     1201 ataaagtttt tggttttgtc tgttaaaata atatattggt ttaatccact tgtttatata
     1261 atgagcaata aagtaaatct agattgtgaa ttatgttgtg atgaaagtgt tttgactgat
     1321 tgttctttaa aagataagaa agaatatgca ttagcactta taaagtcaat taaacttagt
     1381 aggaattata atagtggtat tttaacaaca gagtttaata agacaagttt agaaaagaga
     1441 cttgacagta ttgttaagaa aaaaggtaaa agtggaatat tggtgttatt gattttgttt
     1501 acattatttt cagttacata ttttaatttt gaaagtatta gttttgataa ttcaaactct
     1561 ttaaggaata taggtatttt agaaaaatca ggagccgatc ttaaggaaga aaatgatatt
     1621 agcatgagaa ttgtaaatta atagattaat atatgtaatg aaaaggtata ttgtcatatt
     1681 attaataaat taggaggaat tatgaaatga aaaatttaaa aaaaatttta atagttacag
     1741 gctgtctaac tttaatatct actagtttgg tatttgcaaa tactactact gatgtagata
     1801 catcaaagga tgatgattca gtaataagtt catgtactat aactggttat gaatttactt
     1861 atgaaaacgc accacaatct gtaaaatttg attatgaaaa aaaatgtgaa gcgctaaata
     1921 taaaaccatc taaagatgat ataatttttg ttaataaaga ttctatagat gtaaatgata
     1981 tagaaaacta tgttgagcca aaagcatata ttattagata taaaaaaggt tatgtttttg
     2041 aagttactgg acctaagagt tatacactag atgtaagtga tactgtagta ggatataatc
     2101 gtgttgaatc aggtaatcct gtgcatttag tccaactttt atgtagaact ttagatggtt
     2161 caagtattaa ggatgatagt gaatttggac ctgctacata taatgcagtt aagtcacttc
     2221 aaggaagact tggattaaca agagatggaa ttgttggaag aagtacttgg gatgcagctg
     2281 gaagacgtct ttaaaaaaga gagctattgc acagataatt ttaaattgtt aaatctgtaa
     2341 acatttatgt gtattcttta aataaaaatt tgttttttaa gtgataatta aatttttact
     2401 gatagggcta tggctttaaa aatcatagcc ttatttcgat gttttttcgt ttttgtaaaa
     2461 ttattgccat tttaagaaaa tttattgatt tttttgaaat atttctttat taatttagag
     2521 ttaaatttct ttctttaaaa taaatactta aatgagctaa aacttgagtc caatttctaa
     2581 ttttatgaga ccgtttttca aaaatatcca tagttgctaa gtatagtgat ttgtgcaaag
     2641 aatcatctga aggaaaaata gacttcgatt tagtaacttt ttttaataat ctattgtaac
     2701 tttccattga gtttgtagta tatatcaatg ttcttatcca tgaggatatt taaaatatgt
     2761 tgcgaactca ttccaattgc ttcgccaatt tcttaatgct acctcttctg taattgcatc
     2821 atatactttc tttaatgagt ttttttacat ttcgtttttg ttaaacataa aaagtgagcg
     2881 ccttcctttg tatagtgata tttgatattt taattattat tactttttag aataatacac
     2941 actttatttt aaagaccctg aaaatgatat ttttatacat tatatttgtt atgcataatt
     3001 ttacttagaa cataaactta tataaaacaa taaaaagtat atatattaga taagtaaaaa
     3061 aagcgttctt acagaacaaa tttaatttgc tcatgcaaaa gctctttttt tataatttat
     3121 taataaggta taattataaa aatcttattc aataagattc ataattaatt ta
//
LOCUS       DAMJVM010000174         3038 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.179, whole genome shotgun sequence.
ACCESSION   DAMJVM010000174 DAMJVM010000000
VERSION     DAMJVM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.179"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            169..1062
                     /locus_tag="PC523_003433"
     CDS             169..1062
                     /locus_tag="PC523_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF2476913.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREINKADINVSKYIKYAD
                     LASKNNVQVNWKYVASIVAVLNKNKLKNVKDSQIQEVSDLFVKNFSKNNKVNKLSYVL
                     DELKFNNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKSNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
     gene            1506..1997
                     /locus_tag="PC523_003434"
     CDS             1506..1997
                     /locus_tag="PC523_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HDF2476914.1"
                     /translation="MYENNKVIYNFERCEKLEKTLYLSQFNTYKNFKLTSKKTVINSF
                     ETYVCNMNIRSISLVDTIECISLEDEQLTGKKLILVGDIDTDVILDYVANKRNRNSSK
                     KNILKLKIPFSTFIQIPRKIENKDKINLKYLIQDISSSILDDNNLFISVTAIISYENS
                     SQI"
     gene            2028..2975
                     /locus_tag="PC523_003435"
     CDS             2028..2975
                     /locus_tag="PC523_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476915.1"
                     /translation="MRITKTTFNDVFSNDTNGSILKVEKYVDKKCASVFDILTYTIIV
                     TNVSRYKTGNIFFKDYISKYIEFVNNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNIDKFPTRISIESNRVYTNINKIVFKQLNISN
                     TLKAPKNLKDILKVNANSKILDVKPIKNPTNKGNNLKSCNLIVFGSVDVEVDYSCKNR
                     SKFKTVNKSSDIQKDKHYSNDIQNNNKTISDCKRQISIGNNKVKKITKTFGFSCFICS
                     PVGIVYEDMKNINIKIEHTSINELNPDELFINTSLLLYY"
BASE COUNT         1328 a          271 c          419 g         1020 t
ORIGIN      
        1 tattttaact attttgtatg atagaaaact atatattata acaatagtag tgcttttaat
       61 taaatatatt tcataaatct tcataaaatc ttcaaaattt cgtaaagaat atgtcacaaa
      121 agtaaggtat attatattca gcagataata taagggagga caatatttat gaagaataaa
      181 aaattcattg ttataatact gataatatcg attttaatag gtgtattagt gaaggaatat
      241 tcatctagag agataaataa ggctgatata aatgtcagta agtatataaa atatgctgat
      301 ttagcaagta aaaacaatgt acaagtcaac tggaagtatg tagcttctat tgttgctgtg
      361 ttaaataaaa ataaactaaa aaacgttaag gattcacaaa tacaagaagt atcagattta
      421 tttgttaaga atttttctaa aaataataaa gtaaataaat taagttatgt tttggatgag
      481 ttaaaattta ataatagaca aaaaagattg gttgataatt atatagacaa ccttaaagat
      541 tatggtataa aaccagaaag attgaaatct gatacaaaat atatgaagtt tattgacgaa
      601 attaagacag aagcaataca aaattataag gattataaaa tacttccatc tataaccatt
      661 gcacaagcca taatagaatc tagctggggt aagtcaactt tggcaaaaca atataataat
      721 ctatttggta taaaggctga cgcttattgg aaaggtaagt cagtgacttt agaaacgaaa
      781 gaacatttag atacaattat agatgataaa tttagaatat atgatgataa aaatgaatct
      841 ataaaagacc atgcaaaatt tttagccaca aataaaagat ataaatcgaa tggtgtattt
      901 gatgcaaaaa catatatata tcaagctaaa gcattggaaa aggcaggtta tagtactgca
      961 aaggatgaaa atggaaatag catatatgca gctcgtctaa tagaattaat tcaacagtat
     1021 aatcttcaat taatagacag tgaaattcaa agtgaaatgt aactttgatt aaacttttat
     1081 tgataaggct atgattttta aaatcatagc cttattttga tatgttttca cttgtaatta
     1141 ttaaagcgat attattatct ttcaaaattt cttttacagt acaaatcaaa ttatctaagt
     1201 taaaacttgg agttatttat aaaaggatta tttattttat ataaattttg catttaaata
     1261 actcttattt ctttaaggtt gacagttagt ctataaaaat tataattcct tataaaaaag
     1321 tcaatagata tatacaataa aaaattctca aaaagacttt tacttgcaaa ttgaaattaa
     1381 tgaagtaaaa aagtattctt tattaaaagt agatggcaac tatatagtga ataataaaaa
     1441 atggtaattt gaaataattt ttatgaatag tattaatata tagatacagg gagattgatg
     1501 cgattatgta tgaaaataat aaggtaatat acaactttga aagatgtgaa aaactagaaa
     1561 agactcttta tttatctcaa tttaatacat ataaaaattt taaattaact tctaaaaaaa
     1621 ctgtaataaa ttcatttgag acatatgttt gcaatatgaa tattcgcagt ataagtcttg
     1681 ttgatactat agaatgtata tcattagaag atgaacaact gacaggaaaa aagttaattt
     1741 tagttggtga tatagatact gatgtaatat tagattatgt agccaataaa agaaatagaa
     1801 attctagtaa aaaaaatata cttaaattaa aaataccctt tagtacattt atacaaatac
     1861 ctagaaagat agaaaataaa gataaaatta atttaaagta tcttatacaa gatatttcct
     1921 catctatttt agatgataat aatttattta ttagtgttac tgcaattata agttatgaaa
     1981 atagctctca aatatagtaa tatttaaatt atagaaaggt ggatgcaatg agaataacga
     2041 aaactacttt taatgatgta ttttcaaatg atacaaatgg aagtatttta aaagtagaaa
     2101 aatatgttga taaaaaatgt gcatctgtat ttgatatttt gacttatact attattgtta
     2161 caaatgtaag tagatataaa actggaaata tatttttcaa agattatatt tctaaatata
     2221 tagagtttgt aaataatact gtaaaagtta atggtattat aaagcgtgga ttagacccac
     2281 aacaaggttt ttatataggg agaatagatg caagttgtaa aaaaataata tcttttaaaa
     2341 gtgtagtttt accaaattcg gcacatagga ttataaagaa tagtgccaat atatactatt
     2401 actacaagtg taatatagat aaattcccaa caagaatatc aatagaaagt aatagagttt
     2461 acactaatat aaataaaatt gtatttaaac agttgaatat aagtaatact ttaaaagctc
     2521 ctaaaaatct aaaggatata ttaaaagtaa atgcaaatag taaaatactt gatgttaaac
     2581 ctataaaaaa tcctacaaac aaaggtaata atttgaaatc atgtaacctt atagtttttg
     2641 ggagtgtaga tgttgaagta gactatagtt gtaaaaatag aagtaagttt aaaacagtaa
     2701 ataaatctag tgatattcag aaagataagc attatagcaa tgatatacaa aataataata
     2761 aaactataag cgattgtaaa aggcaaataa gtattggtaa taataaagta aaaaaaataa
     2821 caaaaacttt tggattttcc tgttttattt gttcaccagt tggaattgtt tatgaagata
     2881 tgaaaaatat aaatataaaa attgagcata catcaataaa tgaattaaat ccagatgaat
     2941 tatttataaa cacatcgctt ttattatatt attaggtatt ttaaatctaa aacttaaaat
     3001 tattaaacga gcatttgatt aaagagaagt atatattg
//
LOCUS       DAMJVM010000175         2942 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.180, whole genome shotgun sequence.
ACCESSION   DAMJVM010000175 DAMJVM010000000
VERSION     DAMJVM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.180"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            <1..1306
                     /locus_tag="PC523_003436"
     CDS             <1..1306
                     /locus_tag="PC523_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDF2476916.1"
                     /translation="YMISLFIIYILVIRWIFSVHEITLNTENFKIARKESVNLIKGRI
                     IRTVIYSLILSLVMAIIGYVIYHIGIISIGIWTKYYTNIGSLKELFINRCISFEDYFS
                     LILSIFIFIVDIAFISATYYEYKGVNISRNNIIKEKKSIPKTIFKLSLIILIFCVESN
                     AYSSHVNGLFNTSFSFNTTTIAHRAGAERAPENTLIALKEALNAKADYAEIDVQETKD
                     GELIILHDSNFKRTTRVDKNVWDVNYDEVRTYDAGSFYYDGKGYYDERFVGEKIPTLD
                     EMIKYSKGRIKLLIEIKLTGHEKGDVTKKVVQLIKENNFENQCIIASMDKTVLQKVKK
                     LDKNLMTCYFMAVAYGDFSKLNYIDVYGIESTFVNTTVVEKIHKMNKKIFVWTVNSYS
                     LIEKMLDLNVDSIITDNPYLVDGAIVWKKNGFINKVSDYLFS"
     gene            complement(2458..2634)
                     /locus_tag="PC523_003437"
     CDS             complement(2458..2634)
                     /locus_tag="PC523_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476917.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     ICLYQVLLLVSRYN"
BASE COUNT         1180 a          266 c          380 g         1116 t
ORIGIN      
        1 ttatatgatt tctcttttta ttatatatat tttagttatc agatggattt ttagtgttca
       61 tgaaataact ttgaatacag aaaattttaa aatagcaaga aaagaaagtg ttaatcttat
      121 aaaaggaagg attataagaa cagttattta ttcattaata ttatctttag taatggctat
      181 tattgggtat gtgatttatc atattggaat tatatctata ggcatatgga ctaaatacta
      241 tactaatata ggatctttga aagaattgtt tataaataga tgtatttcat ttgaagacta
      301 ttttagtttg attttatcaa tatttatttt tatagtggat attgcattta tctcagcaac
      361 ttattatgaa tataaaggag taaatatttc taggaataat attataaaag agaaaaaatc
      421 cataccaaaa actattttta aattatcact aataatatta attttttgtg tagagtcaaa
      481 tgcatattct tcacatgtga atggtctgtt taatacatca ttttccttta atacaacaac
      541 tattgctcat agagcaggag ctgaaagggc tccagagaat actttaatag cactaaaaga
      601 agctttaaat gctaaagctg attatgcaga aatagatgtt caagaaacta aagatggaga
      661 gcttattata cttcatgatt cgaattttaa gaggactaca agagtagaca aaaatgtttg
      721 ggatgtaaat tatgatgaag taagaacata tgatgcagga agtttttatt atgatggaaa
      781 aggatattat gatgaaagat ttgttggaga gaaaatacca acccttgatg aaatgataaa
      841 atattctaag ggtaggataa aattactaat agaaataaaa cttactggcc atgaaaaagg
      901 agatgtaaca aaaaaagttg tacaattaat taaagaaaac aattttgaaa atcaatgtat
      961 aattgcatct atggataaaa ctgtattaca aaaagtaaaa aaactagata aaaacttgat
     1021 gacatgctat tttatggcag ttgcatatgg tgatttttca aaattgaatt atattgatgt
     1081 atatggaatt gaatcaactt ttgttaatac aacagtagta gaaaagattc acaaaatgaa
     1141 caaaaaaatc ttcgtttgga ctgtcaatag ttatagttta attgagaaaa tgcttgacct
     1201 aaatgtagat agtataatta cagataatcc atatcttgta gatggtgcaa ttgtttggaa
     1261 gaaaaatgga tttattaata aagtatcaga ttatttattt tcttaatgtt taccatatct
     1321 aataaaaaag agatattatt aaaagtacta atatctcttt ttttattaaa aataaaagca
     1381 attattaata taagtgtatt ttattctacg atagtgtgaa agtatacaaa caataataat
     1441 attattgtat tgatataaat aattgtaatt gcgttgtttt atctaacaag tgtatattct
     1501 atatagatag gatatttttc aaggtttcat atccatattt atctactaag tctttatgaa
     1561 atgtagtttt tattacttct atcttacatt ttttcatgaa agctatctcc tcatatattt
     1621 atataagtat atgttctatt ttaagtttaa acaagtacct acttttaagt aaagtactaa
     1681 catttaggaa agttatttcg ttataatata gagattaaat aagaatgaaa aattataaca
     1741 tagaaggttt taataaatat aatttttctc ctatttaaat atgttgtttt gattataaac
     1801 atcattgaga taatacactt aattaataat ataaaagagg ataaaaattt taaaagttta
     1861 atttttggct aaattaacaa tattaatgtt agtctacgat tgtaaaatat attataatgt
     1921 atatttttat aattactaaa taaaataaat taatcaatat agttttgtaa tataaacaaa
     1981 acataaaaaa tatatataaa taaaatgatt gtttttcttg caatatttat ataaatattg
     2041 aaatttgtct agttatacgt ttttattgac cagttacgca gttgatattg aatattctaa
     2101 atattgatgt tattttatag atggtttaaa catactaggg ggattttata aatgttattt
     2161 gtatgcttta tagaaaatgt ataaaatggt aaaaatttag agatatattt gatgaaatta
     2221 cagaatatgt gtatagaatg atgctcatac attaatttag atataaggaa tgtataattt
     2281 gtattgaatg atttattttt atgtgtatat ttttgactgt tttaatgaaa aagtaaagat
     2341 gtactcccaa tacatcttta ctttagcaga agttatagtt acttataaag tacaaaaaaa
     2401 ttatatattt attaaggtag gtaaatctaa aagggttttc ctaggaaaaa cttaatttta
     2461 attatatcga ctcactaata ataaaacttg atacaagcat atttcaacta cagtttctcc
     2521 gtattctgat ataaaatcat ctaactgtgt atctaaaaaa ttacaataat catatgaact
     2581 gcttatataa gcagttaata tatgtgtaca tttgtctatt tctccaataa acatattatt
     2641 catcccccta aaaaatttag taaaagtaaa aattatattt taaaaacaaa ataacaaaac
     2701 ttataaaatt ttttagtact agttactata actctataaa actaaatatt cctagtttta
     2761 ccaatttagt tttattctac aaatatataa tactagaaga tgcttcatta ttcaacatgc
     2821 ttgtttgaaa atgaaaaata ttatgaaatt atatttactg ttttgaaaag aaaaactaat
     2881 aataaataat tttatatgta attatttaaa tgaaaaatta tgcttttaaa ttagctagtt
     2941 tt
//
LOCUS       DAMJVM010000176         2938 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.181, whole genome shotgun sequence.
ACCESSION   DAMJVM010000176 DAMJVM010000000
VERSION     DAMJVM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.181"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            39..2396
                     /locus_tag="PC523_003438"
     CDS             39..2396
                     /locus_tag="PC523_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDF2476918.1"
                     /translation="MGNLNLKRYNCDIKMGLSSEQIQSRIDNNLVNVFDNGKTKTYKQ
                     IFKDNVFTLFNLINIILVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIRDKEKKEIDVQELVLDDIMILKTGNQICADSKVLDGRLEVNESLITGE
                     SDIVIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKVVGIIIIPLGLILFAKQHFGNGDSIVTSIVGTVAAVNGMIPEGLTLLTSIALAL
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDVVMLEELDIDDIMG
                     NICNALKDDNATLNAIRDKYSILDTHKVKNLIPFSSERKYSGVIFEGEGTYLLGAFEF
                     IFKDKYKELKTEVEKYSKKGNRVIVLAHSNSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPMTVSEVARKAGLKTAGNFIDATTLKNDEDIYEAVDK
                     YNVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRTASLFLVKTIFSFFLSILTLFFFSRQYPF
                     VPIQLALIGAVTVGIPSFFLALEANRGRISGNFLLNILKNALPGALCVTINVIIVYNI
                     VEYLGYNSSVFSTMCAILTGVCGLLILRQQCLPFNKERIFLIVSMTIAFIVGILFLGG
                     LFSFVALSKQLILITVVLAVLMPIMIKVMRFVLDEILGAIVPNLN"
     gene            complement(2481..2798)
                     /locus_tag="PC523_003439"
     CDS             complement(2481..2798)
                     /locus_tag="PC523_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDF2476919.1"
                     /translation="MNEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEILAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         1112 a          333 c          483 g         1010 t
ORIGIN      
        1 tttaagtata aaaattgtta aattaaaagg agcagagtat gggtaatctt aatttaaaaa
       61 ggtataattg tgatataaaa atgggattaa gtagtgaaca aattcaaagt agaatagata
      121 ataatttagt taatgtattt gataacggaa aaactaaaac gtataaacaa atttttaagg
      181 ataatgtgtt tacacttttt aatctaataa atattatttt agtatgttta ctagtttcag
      241 ttggttcttt taaaaatacc ttatttatat ttattgctgt aattaatact attataggcg
      301 taattcaaga aataagagct aaaagattgc tcgacaatct atctgttata gtttcaaata
      361 aggtatctgt tataagagac aaagaaaaaa aagagattga tgtacaggaa ttggtactag
      421 atgatattat gattttaaaa acaggaaatc aaatttgtgc agattcaaaa gttctagatg
      481 gaagattaga agtaaatgag tctttaatta caggagaatc tgatattgtc ataaaaaaca
      541 aaggtgattt cttatattct ggtagctttg tagtttctgg agaagctttt gtaagagttg
      601 aaaatatagg aaaagataac tatgcgaata agatagctaa tgatgccaag atttctaaaa
      661 aacataattc tcagcttaga aattcattaa atgcaatatt aaaagttgta ggtataataa
      721 ttattcccct agggcttata ttatttgcaa agcaacattt tggaaatgga gattctatag
      781 ttacctctat agtaggaact gtggcagctg taaatggaat gataccagaa ggactgacac
      841 tacttacaag tattgcactg gctttaggaa ctataaagct tgcaagtcat aaaacgttag
      901 tacaagaatt atactgtgta gaaactcttg ctagggttga cacattatgc cttgataaaa
      961 caggcactat tacagagggt aaaatgcaag ttgatgatgt agtaatgtta gaggagttag
     1021 atattgatga tataatggga aatatatgta atgctcttaa ggatgataat gcaacactaa
     1081 atgcgataag agataaatac agtattttag atacacataa agttaaaaat ttaattcctt
     1141 tttcatcaga aagaaaatat agtggtgtca tatttgaagg tgaaggaaca tatttactag
     1201 gagcatttga gtttattttt aaggataaat ataaagagct taaaacagaa gtagaaaaat
     1261 attctaaaaa aggcaatagg gtaatagttt tagctcatag taattcttat atggaagaca
     1321 acaatattcc tgaagacata aaaccactag catttatatt aatattggat aaaattagag
     1381 atgaagctaa ggaaacatta gaattttttt ataatgaagg tgtagatata aaaattatat
     1441 ctggagacaa tccaatgaca gtaagtgaag tggctagaaa agcaggtctt aaaacggctg
     1501 gtaattttat tgatgcaaca acacttaaaa atgatgaaga tatatatgaa gcagttgata
     1561 aatataatgt ttttggaagg gtaagcccac agcagaaaaa acaaatgatt ttagcattga
     1621 aaaaacaaaa tcatacagta gctatgactg gagatggtgt aaatgatgtg ctagcactta
     1681 aggaagctga ttgtagtatc gctatggctt ctggaagtga tgttactaaa aatgtttcta
     1741 atttggtatt gttggattca aattttgcat caatgccaaa ggttgttatg gaaggaagaa
     1801 aggtaattaa taatattcaa agaacagctt ctttattttt agtaaagaca attttctcat
     1861 ttttcttgtc gatattaaca ttatttttct ttagtagaca atatccattt gtgcctatac
     1921 aactagctct aattggcgct gtaacagtgg gtataccatc attcttctta gctcttgaag
     1981 caaatagagg tagaatttca ggtaactttt tactcaacat attaaaaaat gctttaccag
     2041 gagcactttg tgtgacaata aacgtaatta ttgtttacaa tatagttgaa tacttaggtt
     2101 ataattcaag tgtattttct acaatgtgtg ctatattaac tggtgtgtgt ggacttttaa
     2161 tattaagaca acaatgttta ccatttaata aagaaagaat atttttaatt gtaagtatga
     2221 ctattgcatt tattgttggg attttatttt taggtggatt attctcattt gtagcattga
     2281 gtaaacaatt aattctcata actgtggtac tagcagtatt aatgccaata atgataaaag
     2341 taatgagatt cgtattagat gagatactag gtgcaatagt tccaaattta aattagattt
     2401 aataatatat aaaaagagca gtcttcattg aactgctctt tttagatgtg tttaatttta
     2461 tgtaagaata tgtagcttta ttaatcagct atatatgagc aacagtagtc aacgacttca
     2521 ttaactttta aatcaaaact tgaatcacta ggaacattaa atttttgacc ttctttataa
     2581 gttacaaact cattgcttcc aggcaatttt acatccatac ttccagcaag tatttccatt
     2641 atttcttctt ctctagtaga aaaagtatat tctcctggca acattaatcc taaagttttt
     2701 ctctctccat taggtagtat tattactcta ctgcttactt taccatcaaa ataaacgttt
     2761 gcctttttta ctgcagtaac attcttaaat tcattcatta gataaaacct ccaaaattat
     2821 ttgtatatat acattatata aaaataatat aaaaaatgct atatatttac aaataaattt
     2881 agtatttgtt aaaaaaatta ataaaaataa aaaatgagta aaattactta aattgata
//
LOCUS       DAMJVM010000177         2888 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.182, whole genome shotgun sequence.
ACCESSION   DAMJVM010000177 DAMJVM010000000
VERSION     DAMJVM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.182"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            <1..655
                     /gene="pgmB"
                     /locus_tag="PC523_003440"
     CDS             <1..655
                     /gene="pgmB"
                     /locus_tag="PC523_003440"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDF2476920.1"
                     /translation="DGVITDTAYYHYMAWKKLAHKIGINIDTKFNESLKGISRMESLD
                     RILEFGNKKDLFSKEEKLKMSEEKNNYYIALINDITSSDILPGIESLLIDVKSNNIKI
                     GLSSASKNAINVLNHLGISDKFDFIANAAKCKNNKPHPDIFLMSAKGLNVNPKNCIGI
                     EDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYNEYMVRRII"
     gene            652..>2888
                     /locus_tag="PC523_003441"
     CDS             652..>2888
                     /locus_tag="PC523_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDF2476921.1"
                     /translation="MKCLFSKIDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGIRIFIDDIELDL
                     AKCKVEDFHRELDMKNGILNRRFIVNINDKRFEARVTRFLSISVKELAVIKYSIKALN
                     SNAKIKFVPYLDSNIKNEDSNYEEKFWNGISSNSLEYKGSILSQTKENAFNTPVFTVG
                     SMMYIKTNGEVGAISHESNYNYCENSITMDALKNEVASIEKYVSITSTRDYKENELLR
                     KCEDILNRELSKNYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     ENAKKLGLKGALYPMVTFDGAECHNEWEITFEEIHRNGSIIYAIYNYTNYTGDYKYIK
                     EKGIDVVIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNVNNNWYTNYIAKWCLDY
                     AVENILRLEKEYNECIDRNNVTREEIDFWKIISKKMYLPYDEELQIIIQNDNFLDKEF
                     IEVKDLPKENLPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWDETLSFAPKLPKEWKSYSFNINYRDNIINVDVCSDLIKIENLKGK
                     AIKIIV"
BASE COUNT         1190 a          273 c          541 g          884 t
ORIGIN      
        1 agatggtgta attacagaca ctgcatatta tcactatatg gcttggaaaa aactagcaca
       61 taagattggt ataaatatag atacaaaatt taatgaatct ttaaaaggca taagtaggat
      121 ggaatcatta gatagaatat tggaatttgg aaacaaaaaa gatttatttt caaaagaaga
      181 aaaattgaaa atgtcagaag aaaaaaataa ttattatata gcattaataa atgatataac
      241 ttcgagtgat attttgccag ggatagaaag cttgttaatt gatgtaaaat ctaataatat
      301 aaagatagga ctatcttctg caagtaagaa tgcaataaat gtattaaatc atttgggtat
      361 aagtgataaa tttgatttta tagctaatgc agcaaaatgt aaaaacaata aaccacatcc
      421 agatatattt cttatgtcag cgaaagggct taatgtaaat ccaaaaaatt gtataggtat
      481 agaggatgca agtgcaggta tagacgctat aaactcagct aatatgtttt ctgttggtgt
      541 tggaaattat gagaacttaa aaaaagcaaa tttagttgta gattcaacaa atcaattaaa
      601 gtttgaatat atacaagaaa agtataatga gtatatggtt aggagaatta tatgaaatgt
      661 ttatttagta agatagatga gtggaagatt atacaagata agattgaaat caaagaaaat
      721 agattagcag agtccataat gagtataggt aatggatata tgggtatgag aggaaactat
      781 gaggaaaatt atagtggaga tacacacaag gggtcttata tagctggagt atggtttcca
      841 gataaaactc gtgttggatg gtggaaaaat ggatatccag aatactttgg taaaattcct
      901 aattcagtca actatatagg tataagaatt ttcatagatg atatagaatt agatttagca
      961 aaatgcaaag tagaagattt tcatagagaa ttagatatga aaaatggtat tttaaatcgt
     1021 aggtttattg taaatataaa tgataaaaga tttgaagcta gagttactag gtttttaagt
     1081 atatctgtta aggaactcgc agtaattaaa tacagtataa aagcattaaa ttctaatgct
     1141 aaaataaaat ttgtaccata tttggattca aatataaaaa atgaagattc aaactatgag
     1201 gaaaaatttt ggaatggaat ttcttcaaat tctttagaat ataaaggaag tattctttca
     1261 caaacaaagg agaatgcatt taatacacca gtattcactg tgggctcaat gatgtatatt
     1321 aagacaaatg gtgaggtagg agctatatct catgagagta attataatta ctgtgaaaat
     1381 tcaataacaa tggatgcttt gaaaaatgag gttgcaagta tagaaaaata tgtatctata
     1441 acttcaacta gagactataa ggaaaatgag ttattaagaa aatgtgaaga tattttaaat
     1501 agagaactat ctaaaaacta tgatgatata ttaaatgaac atacaaagtt atggaataaa
     1561 agatgggaaa gtgcagatgt aaaaataaat ggagataaaa gtagccaaca aggtataagg
     1621 tataatttat tccaattatt ttcaacatat tatggagaag attcgcgatt aaatataggt
     1681 ccaaagggat ttactggcga aaagtatgga ggagctactt attgggatac agaagcattt
     1741 tgtgttccag tatatctagg tgtagcagat aaggaggtaa ctagaaatct tttagaatat
     1801 aggtacaatc agttagaaca ggcaaaggag aatgcaaaaa aactaggact taaaggtgct
     1861 ttatatccaa tggttacatt tgatggagca gaatgtcata atgaatggga gataacattt
     1921 gaagaaatac accgaaatgg aagtataata tatgcaatat ataactatac aaattataca
     1981 ggtgactata aatatataaa agaaaaggga atagatgtag taatagaaat agctagattt
     2041 tgggcaagta gagttcattt gtctacaaaa aaagatttgt atatgataca tggagttaca
     2101 ggcccgaatg agtatgaaaa caatgtcaat aataattggt atactaacta tatagctaaa
     2161 tggtgtttag attatgctgt agaaaatatc ttacgattag aaaaagagta taatgagtgt
     2221 attgacagaa acaatgtgac tagggaagaa attgattttt ggaaaataat atcaaagaaa
     2281 atgtatctgc catatgatga agaattacaa ataataattc aaaacgataa ttttttagat
     2341 aaagaattta tagaagtaaa ggatttacca aaagaaaatc ttccccttaa tcaaaattgg
     2401 tcttgggata agattcttag gtcgtgtttt ataaaacaag cagatgtatt acagggaatt
     2461 tactattttg gaaatagatt tactaaagaa gagaaaaaga gaaattttga cttctatgaa
     2521 aaatatactg ttcatgaatc ttctttatct tctagtatct attctattat tgcctctgaa
     2581 atagataact tagaaaaggc ttatgagtta tactcaagaa cagctaggct tgatttagat
     2641 aattacaata atgatacaaa tgatggactg cacataactt caatgtctgg ctcatggctt
     2701 tctattgtac atggatttgc aggtatgaga acgtgggatg aaacgctatc ttttgcccct
     2761 aagttgccaa aagagtggaa aagttattct ttcaatataa attatagaga taatattatt
     2821 aatgtagatg tatgtagtga tttaattaaa atagaaaatt taaaaggtaa agcaataaag
     2881 attattgt
//
LOCUS       DAMJVM010000178         2801 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.183, whole genome shotgun sequence.
ACCESSION   DAMJVM010000178 DAMJVM010000000
VERSION     DAMJVM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.183"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            118..873
                     /locus_tag="PC523_003442"
     CDS             118..873
                     /locus_tag="PC523_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2476922.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQITLDNTTITNLAEYKRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQSLSLIM
                     SSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQYGDRL
                     IMLHKGEVVLDVKDKEKEDLTVEEILEKFEYAV"
     gene            969..2006
                     /locus_tag="PC523_003443"
     CDS             969..2006
                     /locus_tag="PC523_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2476923.1"
                     /translation="MIKSKKILSLIIAGVLGISMLTGCSKNDGSNASNENKKTDIKNQ
                     KNIGISQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     ITSDENAKSAK"
BASE COUNT         1108 a          361 c          411 g          921 t
ORIGIN      
        1 taataactgc catagttata atagctttct tagctactgg taacttaaat atttcactaa
       61 aaaaaaacaa atgcaaaact agtaccaaaa ataaatcaga aaaaagaggt gttatagatg
      121 ttacaaatta aaaatctttc aaagagtttc cctaatccat acggtgaacc taatactata
      181 tttgaaaatt tatcaatcga tatagaagat ggtgaatttg taagtatcat aggtagtaat
      241 ggaactggta aatctacttt attaaatata atctctggac ttataaaaga atcttctggt
      301 caaattactt tagataatac cactataaca aatttagctg agtataaaag aactcaaata
      361 gtaagtagag tttttcaaga cccaagcctt ggaacttgtc cttctatgac ggttagagaa
      421 aatctatctt tagcacttaa taaagggaaa ttattaaact taaagaaatg tcttcgtcat
      481 aaaacgaatg atttggaaca cttacttgaa ggaatttctt tggacttaaa aaaatactta
      541 gatattaaag tacaatatct ttcaggaggt caaaggcaat cactctcact aattatgtct
      601 agtttagcaa gtcctaaggt cttattgcta gatgaacata cagcagcact tgacccaaaa
      661 acatcaaatg aagtaataga gttaacagat aaaatcgtaa gagaaaaaaa tataactaca
      721 cttatggtta ctcacaactt aaaacatgct cttcaatatg gtgatagatt gataatgctt
      781 cataaaggtg aagttgtctt ggatgtaaaa gataaagaaa aagaagattt aacagttgaa
      841 gaaatcttag aaaagtttga atatgcagta taagaaatta aaatcttagt tttaatcagg
      901 aatatttata atctattata tgaatatatg aacaaatata attattaaat tttgagggag
      961 gaaataaaat gataaaaagt aaaaaaatat taagtttaat tatagcaggg gtacttggta
     1021 tatcaatgct tactggatgt tccaaaaatg atggctctaa tgcctcaaat gaaaataaaa
     1081 aaacagatat caagaatcaa aaaaatatag gtattagtca attggtagaa catccatctt
     1141 tagataaagc taaaaaagga ttcatcaaag cactcgaaga taaaggctat aaagacggag
     1201 ataatataaa aatagatttc caaaatgcac aaaatgatat gcctactaca caaagtattg
     1261 ctagtaagtt tgcatccgac aaaaaagatt tgatatatgc tatatctact ccatctgcac
     1321 aggcagctta taatgctact aaagatatcc cgataataat gactgctgtt acagaccctg
     1381 tagaagctgg acttgttaaa tctcttgaaa aaccaggtgg gaatgtttct gggacatctg
     1441 actatctttc aattgataaa acattagaat tagttaaaac attgactcca aaagcaaaga
     1501 aaataggagt tatatacaat actagtgaag ttaattcaaa aatccaagtt gattctctac
     1561 atgattatgc taagaaaaat aattatgaag tagttgaaaa aggaatcagt tcttcaagtg
     1621 aagttaacca agctatttct agtttagttg gcaagataga tgttttatat gtacctactg
     1681 acaatttaat agtttcttct atgccaatag tttctaaagt tgctaatgaa aacaagattc
     1741 ctataatagc ctctgaagaa ggttctgtat cttctggtgc tttagcttgt tgtggaatag
     1801 attatgaaaa actaggttat aaggctggag aacttgctat tgaagtatta gaaggaaaat
     1861 ctgttggcga tatacctgtt actacattag atgaaactga aataataatt aacgaagata
     1921 cactaaaagc acttgacatg caaaagttat cagcagataa tataaagtat ataacgtcag
     1981 atgaaaatgc aaaatctgca aaataaaact tattaatgtc aaaaaatatg taatatgaag
     2041 taggtaatat gaaaacttta tataataagt actcttatcc accctaaccc tttgaaaatt
     2101 gtgcttattt tagcaatttg aatattgcat ataaagttat tcattttcca taaaagtcgt
     2161 ctatattgac gactgattat gaaagttata taactttcta atcactactt gagctgtcca
     2221 aaagaatttt aatattctct agacagcttt tttttatgag atttgattga tatatatgtc
     2281 tagttattcc atacttagta aatattttga taaaatttcc acatccacca caacttagga
     2341 taacgttttt tccaaataat atattaattt aaaagtattc taagtatata ttatttagta
     2401 ttttaattaa atttagcagt tgacttttat tattgtataa tttataattt ataacaagtc
     2461 gaaaatacat ataattttct gactaattaa aatattaaaa gggagagata attatgaata
     2521 ctttattaaa caataaaaaa tattattatt attatagcca ctatttttag ggtttgtacc
     2581 tacgttttaa catagataca gaccccttaa gactactaaa attttgggat tgtatctatg
     2641 ttggctatat tgtttataaa gttttcatgt aactatataa gagccacata gattaaacta
     2701 tgtggcttat tcgtgttttt accacatagt tttgtacact atgtggtttt tttatacaca
     2761 aaattagcaa ataattaaaa ttataaaaat attttcaaag g
//
LOCUS       DAMJVM010000179         2702 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.184, whole genome shotgun sequence.
ACCESSION   DAMJVM010000179 DAMJVM010000000
VERSION     DAMJVM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.184"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            152..2359
                     /locus_tag="PC523_003444"
     CDS             152..2359
                     /locus_tag="PC523_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HDF2476924.1"
                     /translation="MYSKIGGINLKKIKKKRIALLLATITTLTFNQKFLVHAYENNYN
                     SEEKLWLPDFTDNDRAKQLEISKLTEKVNLNSIALERIKNINSKQIIENVSPAKLGDE
                     IVKQGDTSNFSLEDNSENIKLPSNVDNSQTKYFPNIGSQGSLASCASYATAYYTMTYM
                     TALVNDWSVKDVSGKNIDSKIFSPKWSYNFINDGKDAGAFAYQNYNVFKTHGCATLAD
                     FPYVGLGRDPKNYREWAKDENIWRNATKYQVDEILRVNFNKNNKPNSFITSNKDSNLD
                     DAKRLLLNGYILNFYTWFSDANYKQISNDPSTKDDDSYVNQKAIYQINNQGAHEMTIV
                     GYNDNIWIDINSNGKVDDGEKGAFKIANSHGPDYANNGFVWMAYDALNKKSSVLDFTN
                     SNARQGAFLNNEAEYVVISKKETPSKLVRFTMQHDNRKQLQVSLVYEKNGNKQTVVYS
                     PLLSYSGGDFPLDGVNNGSEITFVIDLAHLEKELNVSLSSADKIYLQIKDNLKDDKVA
                     IIKSIILVDNKGHKLSPELISKNVEINGDSKMFDLGQIDNSSELYLQSPEISTDKTLS
                     NNGKFNINVSVPPNNSATKLELFENNVPIETKDLVRSSELQNINFDMSDKKEGFYTYF
                     AVLEDGNGKCLKSEEVNIIVDSIIPPSIKISPNPNKVDVEVIVPAMNNAKTLNVYMDG
                     QKIVNEELSVRNPNEVKKSFVLYSLIVGTHTFWAEVEDENATISSQKVSITID"
BASE COUNT         1090 a          297 c          420 g          895 t
ORIGIN      
        1 ttttaaaaac taactatttc caagtaaata caagatgttt tatattgttt ttagttttga
       61 tactatgttt aatcataaaa aaatatcatt tttgcttata tatttttatt gaaataatta
      121 aaattgtgtt gtaaaataaa ttttaacaaa aatgtatagt aaaattgggg ggattaattt
      181 gaagaaaatt aaaaagaaaa gaatagcatt attactagca acaattacaa ctttaacatt
      241 taatcaaaaa tttttggtgc atgcctatga aaataattac aattcagaag agaaattatg
      301 gttaccagat tttactgaca atgatagagc taagcaactg gaaatttcta aattaacaga
      361 aaaggttaat ttaaatagta ttgcattaga aagaattaag aatatcaact caaaacaaat
      421 tattgaaaat gtatctccag caaaattagg agatgaaata gttaaacaag gagacacttc
      481 taatttttca cttgaagaca attcagaaaa tattaaactt ccatcaaatg ttgacaatag
      541 ccaaactaag tattttccta acatagggag ccaaggttct ttggcaagct gtgcatccta
      601 tgcaacagca tactatacta tgacttatat gactgctctt gtaaatgatt ggagtgtaaa
      661 agatgtgagt ggaaaaaata tagattctaa aatattttca cctaagtggt cttataattt
      721 cataaacgat ggcaaagatg caggagcatt tgcttatcag aattataatg tttttaaaac
      781 acatggttgt gctacattag ctgactttcc ttatgtaggt ttaggcagag atcctaaaaa
      841 ttatagggaa tgggctaaag atgaaaatat ctggagaaat gcaactaaat atcaggtaga
      901 tgaaatatta agggttaatt ttaataaaaa taataagcca aattctttta ttacatcaaa
      961 caaagactca aacttggatg atgcgaagag attattacta aatggatata ttttaaattt
     1021 ctatacttgg ttctcagatg ctaattataa acaaatttct aatgacccct cgactaaaga
     1081 cgatgatagt tatgtaaatc agaaagcaat ttatcaaatt aataatcaag gtgcacatga
     1141 aatgactatt gtaggataca atgacaatat atggatagat atcaatagca atggtaaggt
     1201 tgatgatggt gaaaaaggtg cttttaagat tgctaattct catggacctg actatgcaaa
     1261 caatggattt gtttggatgg cttatgatgc attaaataaa aaatcatcag ttttagactt
     1321 tacaaatagt aatgcaagac aaggagcatt cttaaataat gaagctgaat atgtagtaat
     1381 aagcaaaaaa gaaacaccat ccaaattagt gagattcact atgcaacatg ataacagaaa
     1441 acagttgcaa gtaagtctag tatatgagaa aaatggtaat aaacaaacag tagtatacag
     1501 tccattatta tcttatagtg gaggagattt tccattggat ggtgtaaata atggttcaga
     1561 aatcactttt gtaattgatt tagcacattt agagaaagaa cttaatgtta gcctatcaag
     1621 tgcagacaaa atttatttac agataaagga taatttaaaa gatgataaag tagcaattat
     1681 taaatcaatt atcctagtag ataataaggg gcataaacta tctcccgaat taatctccaa
     1741 aaatgttgaa attaatggtg attcgaaaat gttcgatttg ggtcaaatcg ataattcatc
     1801 agaattatat ttacagtctc cagaaatatc aactgataaa accttgagta ataatggtaa
     1861 atttaatatt aatgtttctg ttcctcctaa taattctgcg actaaattgg aactatttga
     1921 gaataatgtt cctatagaaa caaaagattt agtaagaagt tcagaattac aaaatattaa
     1981 ttttgatatg agtgacaaaa aagaaggttt ttatacatat tttgcagttc ttgaagatgg
     2041 caatggtaaa tgtttaaaat ctgaagaagt aaatataata gtagatagta taataccacc
     2101 atcaattaag atatcaccta atcctaataa agttgatgtt gaggtaatcg ttccagcaat
     2161 gaataatgca aaaactttaa atgtatatat ggatgggcaa aaaattgtaa atgaagaact
     2221 aagtgttaga aatcctaatg aagtaaagaa atcatttgta ctttattcat taatagtagg
     2281 tactcatact ttttgggctg aagttgaaga tgaaaatgca acaatatcat ctcaaaaagt
     2341 aagtattaca atagattaga taattttaga tattatggaa aaacatctaa ataaatagat
     2401 gttagaataa gtttatattt aaataacaaa atactatatc ttagtaattt caataattat
     2461 atggggatag caacgatatc tagaaattac attgatgaat tttgttgaaa aaagacaatt
     2521 ttttatggat tgtctttttt tgtcaataaa agtattaaat tactgtctga ctttataaat
     2581 attaaagatg tctttatgta tatagactta attttattga tttctagaaa tttttttata
     2641 tattggtata attattctgt ttaaatttgc ttaaaaagta tattaattat aataagaaac
     2701 aa
//
LOCUS       DAMJVM010000180         2530 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.185, whole genome shotgun sequence.
ACCESSION   DAMJVM010000180 DAMJVM010000000
VERSION     DAMJVM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.185"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(225..1475)
                     /locus_tag="PC523_003445"
     CDS             complement(225..1475)
                     /locus_tag="PC523_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HDF2476925.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMNNNEPLYDFITDDKIEHTVWKIDNDDIICNLVDEFRKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLCDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1507..2448)
                     /locus_tag="PC523_003446"
     CDS             complement(1507..2448)
                     /locus_tag="PC523_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HDF2476926.1"
                     /translation="MYNILVTDGIEKEAAKKLRELNFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLEKADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
BASE COUNT          761 a          490 c          234 g         1045 t
ORIGIN      
        1 aatacacatt taaaaccttc caaatatata aatacaatta tataaatttt catcctcaat
       61 tataacatag aatatgacta tctatatgct aaaattatcc attttcaaat aaaggtaact
      121 attattaaaa atatcaactt ataggattta tatcccatat acgattttca atcaaaaaaa
      181 caagggctaa aaagtgaatt gtacactttt tagcccttta ttaattattc atttatttca
      241 tgtaagaata gcccacatct tactttaggt tcaaaccatg ttgatttagc tggcattatc
      301 ttatttgcat ctgctactct tattaaatct tcaatactag taggatacat tgcaaatgct
      361 actttcatat cttcattaac tcttttttct aattcttcaa gacctctaat cccaccaatg
      421 aaatcaattc ttttatctac tctaggattg tcaattccta aaatagcatc aataacatag
      481 tcttgtaata tagaaatatc taatgagtca acagggtctt ctatttctaa taaattttta
      541 tttgctttca ttttatacca cttatcacat aggtacattc cgaaagttcc ttttttgtca
      601 ggtttatatt tttcttttcc ttctatttct tctagttcaa aattttcttt tattttattt
      661 ataaattctt cctcgcttaa accatttaag tctttgataa ccctattata atccaaaacc
      721 attaaatttt catctggagc aatcattgct ataaagtaat taaattcttc ttttccagta
      781 taatttggat ttttagctct catttctttg gctacatttt ctgctgaagc tgtcctatga
      841 tgaccatctg caatgtatag ataatctaat tttctaaatt catctactag gttacatatt
      901 atatcatcat tatcaatctt ccatactgta tgctctatct tgtcatctgt tataaaatca
      961 tataaaggtt cattattatt cataaaatca ttgattatat catctatcac cctttgatgt
     1021 ttataagtta caagtatagt tcccgtatta gcatcacagt attttatgtg tttagttcta
     1081 tcaatttcct tttctggtcg agtgtactca tgttttttta ttgttccatt caagctttca
     1141 tcaactgata tacatgctac tataccaact tgagctctac catccatgat ttgtttatat
     1201 atatatatac actctttatc atcttttact agaatttctt gttttcgaaa ctcatctaaa
     1261 ttttctcgcg cttttaaata aactttttca tcatataaat ctatattttc atctaaatca
     1321 atctctgatt tatctatatg caaaaatgaa tatggattcc cttctgcaat tcttctagct
     1381 tctttactat tcataacatc gtaaggaagt gctgctactt tatctactat atatttattt
     1441 ggtctaatcg ccttaaatgg tttaatcgtt gccattacta atttcctcct aattatacaa
     1501 aatattttat aatgctaccc ctattagatt gttgaagtca agaaagaaat cttgaatcac
     1561 atttactatt tcgtctccta ttctttcttg agcttctact gtagatgccc caatatgagg
     1621 tgtaggagaa actcttggat gatttactaa gtctagattt aatgtaggtt cttgttcata
     1681 aacatctata gcagcagctg ataatttacc ttcatctaat gcttttagta aatctttttc
     1741 atcaactaaa ccacctcttg cacagtttat taaataagcc cctttcttca tcttatttat
     1801 ttctttttca gttattactg cccccttatt cttatcaaat ggtatgtgta aagataagaa
     1861 atctgccttt tctaaaactt cttccatatc acagaattta taattgttaa agccttgcgc
     1921 ttcacccatt atgtcagtat atataacatt catacctaat aattctgctc tctttgcaac
     1981 ttcttttgca atccttccaa aacctataag tccaagtgtc tttccattaa tttctgtacc
     2041 tttatatttt ttcttctccc attttccatc tctcatagtt acatttgcag tatttatgaa
     2101 tcttgcaaga acaaataatt gacctattgt aagctctgca acagatatac tgcttgcatt
     2161 tggagtattc ataactttta tgccattagc ttgagcatat ttaacgtcta tattatctag
     2221 tcctactcct cctcttacta tcaacttcaa ctttttaccc tctaatgctt tatctattac
     2281 atctttagta accttagtag ctgaacgtac tacaagtaca tcaacatctt taagtttatc
     2341 tcctaaaaca tctttttcat aaaactgctc tatcacatta aaatttaact ctctcaattt
     2401 tttcgctgct tctttctcta ttccatcagt aactaatata ttatacataa caaactcctc
     2461 caatttttat tcacttaatt tttccaattt ttattttttt aagtattttc atcaataaaa
     2521 cactttactt
//
LOCUS       DAMJVM010000181         2419 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.186, whole genome shotgun sequence.
ACCESSION   DAMJVM010000181 DAMJVM010000000
VERSION     DAMJVM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.186"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            108..917
                     /locus_tag="PC523_003447"
     CDS             108..917
                     /locus_tag="PC523_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2476927.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELNLIDKKVAELISHKEN
                     IIKRLSSIESAVKNTKIDEIEVIYIKKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKVYMEKVFEFIEENNYKVIADPIEIYKIDIHETGIVEEFITEIQVPIIK"
     gene            complement(1026..1235)
                     /locus_tag="PC523_003448"
     CDS             complement(1026..1235)
                     /locus_tag="PC523_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476928.1"
                     /translation="MGNNDKEKSKNSKNIFHDIPVLVNNFFWSMIGFIVIKNFGNGNF
                     FTYLLGIGAGLLWGIFIQKFFTNNK"
     gene            complement(1721..2395)
                     /locus_tag="PC523_003449"
     CDS             complement(1721..2395)
                     /locus_tag="PC523_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF2476929.1"
                     /translation="MNHYILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     KADSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
BASE COUNT          934 a          287 c          334 g          864 t
ORIGIN      
        1 tatccttgtt ttagagattt ttttagtata ttctaatact atagtattat agagtaataa
       61 tgttaattta aaataaaaat agggttatgg aggagtttat tatataaatg aaagattact
      121 ataaaatagg agagatatct aaaatatatg gaataggcag agattcatta atgtattatg
      181 aggaaatagg gattctaaga ccagttagag atataaatgg gtatagaatg tataatatat
      241 cagacatatg gaaactaaat ttaataaaag aatttagaag tttgaacttt ccaatgaaaa
      301 aaataaaaga atatttagat gatagaagta tagagtctac gaaaaacatc ttaaatgagg
      361 agcttaattt aattgataaa aaagtagctg aacttataag tcataaggaa aatattataa
      421 aaagattaag ttcgattgaa agtgcagtta aaaatacaaa aatagatgag atagaggtca
      481 tatatataaa aaaaagaaaa gctttagaat taaatgctga tataaagaga gatgaagatt
      541 ttgacttttt aattcaaaag cttcagaaag aatatgaaga tagatttaat atacttggaa
      601 acaacaatat tggttctgct ttttctactg aagcaattaa taaaggtata tttaatgaat
      661 ttaaatctgt gttttgtttt ctggaaagta atgagaaagt atacaatata gtttttgatg
      721 aagggtatta tgtgacttta aattatactg gaagtaattc taacaataag gtatatatgg
      781 agaaggtatt tgaatttata gaagaaaata attataaagt aattgctgac cctatagaaa
      841 tatacaagat agatatacat gaaactggaa ttgtagagga gtttataact gaaattcaag
      901 ttcctataat taaatagcta gatttcaaca aattagatga taaatagtat aaaaaaatga
      961 gtttaccact atattataaa agtagtggta cattttttat ttgggatttt ttgtttaaga
     1021 tgtgtttact tattattagt aaaaaatttt tgtataaata ttccccacaa taaacctgct
     1081 ccaataccta ataaataagt aaagaagttt ccattcccaa aattttttat aacaataaat
     1141 cctatcattg accaaaaaaa attgtttacc aatactggga tgtcatgaaa tatgttttta
     1201 ctattttttg atttttcttt gtcattatta cccataatgt catctcctta ccataagcat
     1261 tgaacactgt gcattaccta gaaatcattg aaatatttaa actaattttt tagcataaga
     1321 ttatttataa attagattaa aataaaaagg atatacgttt ttacaactag ataaaataac
     1381 tcataatata ttttataaat atcagtacat atagctttat atcattaaag ttctctagtt
     1441 ttgatgattt agtttaattt tatagtttaa tagtacaata gaattattta ttgttcaaca
     1501 tatgtatttt aaagttgaaa aaatttatag atatgaagac tattgtacaa ctaaaaatgc
     1561 actagatgtc tagaactatg gcaactatac tgtagttttt taataaaata tatatgtgaa
     1621 ttgataaaaa taagggacga actttatata gaatcatccc ttgatatctg catttttatt
     1681 ttaattaaat atattattat ctttaaagtt ctattttaca ttatatttct aatgtttcta
     1741 taagaggatt atcgctaaat aattttaata aatttgtttt tttgtatcct ttaggtaact
     1801 tagtatatat aactacctct aaccaatctc catcaagacg tttaatattc atgtttataa
     1861 tttcaatatt agatttttta aatttacctt caatatattc tatagaatca gctttattag
     1921 aaatcttaag agaaacttgt tctgctgtag ggagtttcat ccatttccta tctctatgaa
     1981 gtgttatctg tacaataaca ataatgatac ttgaaacaat accaactaag tataatccag
     2041 agccaatggc aagacctgta ccggctgttg cccatatacc agcagctgta gtaagaccac
     2101 ttactgattg atttctaata aatattaatc cagcacctaa aaaacctatc ccactgacaa
     2161 cttgtgctgc aacgcgggct gggtctagac caatacctgg agtacccact atatcattaa
     2221 atccatactt ggaaacaatg attataagtg ctgaagctaa tgccactata aaatgagtgc
     2281 gtattccagc ttcttttagt ctattctttc tctcatatcc aattaaagct ccacagaaac
     2341 cagcagtaat gattcttgct atgtattcaa attgaatcaa tatataatgg ttcatttgtc
     2401 tagcacccct ttctatata
//
LOCUS       DAMJVM010000182         2379 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.187, whole genome shotgun sequence.
ACCESSION   DAMJVM010000182 DAMJVM010000000
VERSION     DAMJVM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.187"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            155..295
                     /locus_tag="PC523_003450"
     CDS             155..295
                     /locus_tag="PC523_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476930.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(292..663)
                     /locus_tag="PC523_003451"
     CDS             complement(292..663)
                     /locus_tag="PC523_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2476931.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTNISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            1207..1464
                     /locus_tag="PC523_003452"
     CDS             1207..1464
                     /locus_tag="PC523_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476932.1"
                     /translation="MIIIRSQDKTNLMYINQIKIDGSQVYAVFESNLDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITFNKVRYNVNKIFQMPVK"
     gene            complement(1574..2254)
                     /locus_tag="PC523_003453"
     CDS             complement(1574..2254)
                     /locus_tag="PC523_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2476933.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVVIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSDLFRVDEINLIPFDSVGILTYILNVIMFMPLGFLL
                     PLIWKNFRNIINVLLTGLGFSLAIEFCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
BASE COUNT          963 a          268 c          267 g          881 t
ORIGIN      
        1 tattattata taattaagat aattatctag ggaaattttg gataatcaaa atatatttta
       61 taaaatcaaa atacaattac ataaacttta ttaaaaattt gttgggaatt tctgactaac
      121 attaagaacg taagtttgtt gggggatggt atatgtggaa aagtcaaaca ctactgttac
      181 aaaaaaagaa aaaattgaag atataggaat gaaattagaa catttattga gtgaagatat
      241 tactttttat gaattattac ttgagatttt gtataaagaa aaagaagaga attaattctc
      301 ttctttaagg aatcttttta aataggcatt atcaatcatt ttttctatag cttctctatc
      361 ttctttttca agagaatata ttttttctaa aagttttttt gttttttcac tcactgataa
      421 gttctctatg attaatttat cttctttgct tatgtttgtg ttatcattta cgcctaataa
      481 ataatctgtt gatgtttcta atgcttttgc taatctcact attatttcac ttttaggttc
      541 tcttaaatta ttttcataac gtgacaagct agcctcagtt ataccgactt tagtagcaag
      601 ttctttttga tttatatcta attcttttcg taaatttgct attctatctc ctaaactttc
      661 catatgtaca tcactcctta ccgaaattat aagttatttt tataaaaagt aaaataaaat
      721 taccaaaaat ataaaaaaaa agaattaaaa taagaaaata tattgaaaat taccatttgt
      781 atatgtataa taagaattat acaaatggta atttgatgag aggtggtatt ttgaaataag
      841 aaaccgaatt aattagtaaa catatagaaa gttttgaata ctgttacttg agatataaac
      901 aatatgcata aaaatttttg aaagtaaaat taccaaatgt ataaaataaa tcagtagcat
      961 agatagtttg atgctttaat agattatgta atttagttat aatagccaaa aatgcaattt
     1021 accaataaaa caaggtatta taaaaaaagt attctttaag ggaacttttt attagatatg
     1081 caattgatac aaataatcgt attaaaaaat aaaatactaa gtttaaaaat ttaaataatc
     1141 tagaaatcaa ttataaaata ttatttctta gaaactaaaa ttaattaagt aaatggagga
     1201 attgacatga taataattag aagtcaagat aaaactaatt taatgtacat aaaccaaatt
     1261 aaaatagatg ggagtcaagt ttatgcagta tttgaaagta accttgatac tgtaaaaata
     1321 ggagattatg aaaataatac aagagctata caagtattgg ataatataca aaacttcatt
     1381 gaaaatggaa caaaatatga ttatataaca ttcaataaag tcagatacaa tgttaataaa
     1441 atatttcaga tgccagttaa ataatgagtg tgcattttag tttaataact aaagcccgct
     1501 ttcttttgaa gtatgaaaga gggctttaca catttgtaaa tattgaagta cacttgtttt
     1561 gtcagtaaat tatttaataa ggtataatgg accaattaaa caataaaaat tctcctaaaa
     1621 cagcaagtat taaatatata atagcttcat ttttataaag agaggtaatt tttttgctta
     1681 tatcatcatc aattgtaata gcaatttcat cttgaataat atttttatat atagatggaa
     1741 aaatatcttt tttctttata agattaaata aattatttac acctacccat ataaaatatc
     1801 ctaaaacagc acctaatgtg ttcatcatta aatcatcaat atctgtagct cttaagttaa
     1861 acaattgaca aaattcaatt gctaatgaaa aacctaatcc agttaataaa acattgataa
     1921 tatttctaaa atttttccaa attaaaggta gtaaaaatcc taaaggcata aacataatta
     1981 catttaaaat atatgttaaa atgccaactg agtcaaaagg tattagattt atttcatcaa
     2041 ctctaaaaag gtcactatac cttcctatat cccatatgct accaatacca gctttactaa
     2101 gtgctaggta tatatataat aaaaaaatat atacccatac taaatgtatc actacatttt
     2161 tttcaacttt caaatttttt ctttttataa caactatttg gtatattaag catggtaata
     2221 taacacaaat aaatgaataa aatgtccata tcattttatt ttgtcccctt tcaaatttat
     2281 gtttttagtt ttataagtac tatctttata ttaagcggta agttttaaaa atcttttaat
     2341 aaaatcttaa aaatttatta atttataaaa tatattatc
//
LOCUS       DAMJVM010000183         2377 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.188, whole genome shotgun sequence.
ACCESSION   DAMJVM010000183 DAMJVM010000000
VERSION     DAMJVM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.188"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            141..1739
                     /locus_tag="PC523_003454"
     CDS             141..1739
                     /locus_tag="PC523_003454"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HDF2476934.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDVKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VISVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEVMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDMDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLVLAVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(1830..2042)
                     /locus_tag="PC523_003455"
                     /pseudo
     CDS             complement(1830..2042)
                     /locus_tag="PC523_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888191.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
BASE COUNT          904 a          297 c          347 g          829 t
ORIGIN      
        1 tgtagtattt atgtaagaaa taaattttat ataatatttt ttcactaaat ttttttcttt
       61 ttacaacaaa tactcactac cttttgatat aatattttta tcaattatta ttatattcaa
      121 gttttgaaag gagtattaaa atgagtttat tagttttcgg acataaaaat ccagacacag
      181 attcaatatg ttctgcaatt tcattagcac atttaaagaa caaactagga ataaaagcaa
      241 cagcttatgc tttaggagat atcagaaaag aagctaaata tgccttggat tactttaagg
      301 tagatgcacc tgaaatttta gataatgtta ggatacaagt tagagattta aattatgaca
      361 aagtagtacc tttaacacca acttcatcta tattagaagc ctataacctt atggatgaaa
      421 aaaatgttaa aacactacct gtaacttatg atgatggaac ttttgctggt atcattacta
      481 tgaaagaaat agctaaaaac ctacttcatc aacactttac aactatacac acatcactat
      541 caaacatttg taaaaacctt aatggtacta ttcttgtgga ttgtggtgaa gatgtaaaag
      601 gtagagtagt tactttatca tttggaatgg agactctaat agagacttta aaagagggag
      661 atatagctat tgttggcgat agatatgata gtattgaata tgctattgat acaaaagtta
      721 aacttttaat acttactaat catacagaga tttcaaaaga cttgctagct cttgctaaga
      781 taaatggagt gtctgttatt tctgttgaaa gtgacaatta taaaacttct aatgtaataa
      841 atcaatgtag tttcttagac tctatagaag ctacagaaaa tttaattaca ttcaaagaag
      901 atgattatat ggatgaaata aaagaggtta tgctagagac aaacttcaga tcttatccaa
      961 tattagatga tgataataag tttttaggtt tagtttcaaa aggtcatcta ttaaacccat
     1021 ctaaaaagaa tgttgtttta gttgaccata acgaatatgc tcaaagtgct gatggaattg
     1081 aacaagcaaa catagttgaa ataattgacc atcataaact tggtggtatt tctactgatg
     1141 ttcctatgtc atttagagtt atgcctgttg gatgcaatag tactataatt tatcaaatgt
     1201 acaaagaaaa taatgttgag ataccttatg aaatagctgg gcttctttta tctgctatat
     1261 tatcagatac attattattt aagtctccaa caacaactga tatggataag aaggcttgtg
     1321 aagaattaag taaaatagca aaagtagaca tggaaaaata cgctatggat atgtttaaat
     1381 gtggtacttc tttagatgaa tatactatag aagaaatagt aaatatggat tttaaagagt
     1441 ttaatatgag tggacataga gttggtattg gacaggtatt tactttagat atagactcta
     1501 tatttgctaa aaaagatgag tttttatctt atataaattc gactgattat gataaattag
     1561 ttcttgcagt tacagatata atcaaagaag gttcttattt aatctataaa gcagaagata
     1621 gcttaattgc taacgcattt ggtgttgaag gggttcaagg tacatttgca ccaggtgttg
     1681 tatcaagaaa aaaacaatta gttcctaatt taactactgc aatcaaaaat tttaaataat
     1741 ataaagtttt taaacaataa aaaagaaggt gtctcaaagt gaattttata gtccatgagg
     1801 cactcttttt tgtataaaat caaatatatt attctaaata tcatcaaagt agccgtaatt
     1861 accgctgatt gcccatttcg tattcctact gctatagata aaaaattatt tatagtaaaa
     1921 gtagttcctc caaataaaaa gccagcgaaa tacccagaaa gtactcatca tcatgattca
     1981 gtagtaagac ctgttaaatc tcttcctcct agaattagaa ataaaatagc gaaagaatac
     2041 atacttagtt gcaacataat taaaatttac agtcttaaat ttttatatta ataatgctaa
     2101 tttccacaaa aaaagaacat gtaattagaa tcttctaaca gcatgttctt tttcattatt
     2161 atatattaca ctttaaatta ttccttgact ggcttagctg gtgtaaacta aaccagtctt
     2221 tctaaaatat taagcacttt tttcttatta tgttttttag gatttattgt ctatattatt
     2281 gtaaaccaaa gttataaaat tgtaaataga atatattacc atatcaatat ttttttataa
     2341 gtaaaaatta taaaaaaact tatttttatt aaataaa
//
LOCUS       DAMJVM010000184         2271 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.189, whole genome shotgun sequence.
ACCESSION   DAMJVM010000184 DAMJVM010000000
VERSION     DAMJVM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.189"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(164..1456)
                     /gene="brnQ"
                     /locus_tag="PC523_003456"
     CDS             complement(164..1456)
                     /gene="brnQ"
                     /locus_tag="PC523_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2476935.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFARAGKGLSVALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     DKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     VPLVNNLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          761 a          371 c          249 g          890 t
ORIGIN      
        1 ttctctttat aaattgtact actccaaaac taaatttttc aatttattaa ataggattaa
       61 taatagtctt ttatctacaa taatttaata attcttttaa atataaaagg aattgcctat
      121 ataatttaaa ggcaattcca taattatcaa ttagttttta taattatgca gttttgctac
      181 ttttagatgg aataaagttt cctattattc ctgctattac aactggaact atccagttaa
      241 atcctaatga agcaaatggt aagttattaa ctagaggaac tttcacacca aaacttgttg
      301 caactgttaa tacacttact aataatgcca tatatgcagc acatttgaac acgttatcat
      361 ttttaatttt attactaaat aatgctagta taactagagt tatagttgct ggatatacca
      421 tacttagtat aggagaagag aattgtatta ttgtacttac tccaaagttt gatattattg
      481 cactaaatac acaaactact ataactatat gctcatattt taattttcca tctgtaagtg
      541 ttgagaaata ttgaccagct gctgaagaaa gacctatagc tgttgttaaa catgctaatg
      601 caactataat agctaataaa attttacctg tagttccaag taaagctgct gttattgcaa
      661 ctattaaagc tgtttgttga acatcttgtc catattgttg agacacagtt gctccaagat
      721 aacataatcc accataaact agtgccattc ctattactgc aatcacacct gcacttattg
      781 tggctttcat cttttcttta tcacctgtat aacctttagc tattactgac atgattataa
      841 ttgatgcaaa tacactagct gccaatgcat ccattgtttg ataaccttga gttatacctt
      901 ctgcaaatac attttgtatt aaagaactgt ctcttatttc tcctaatgga gatacgatac
      961 ctttaactat taatactgct aaagctataa gtaaagctgg agttaatatt gacccaacta
     1021 tatctacaac ttttgatggt cttattgtta aaaccaatac taaagcaaag aacactatag
     1081 agaatatcac tggacttatt ccagaaccta ttgtaggtaa tatacccatt tcataagttg
     1141 ttgctgctgt tctaggtatt gctaaaagtg gccctataca gattaccatt gcacatccta
     1201 atgcaacact taatcctttg cctgctctgg cgaacatctt tgatgtgtct ccattgaact
     1261 tagttgctgc tataactgct aataatgcca aaccaccatc tgcaaaaagg aatcctatga
     1321 aagctactag ccagctactt ccagataaaa ctcctaaata tggagggaag attaagttcc
     1381 ctgcaccaaa aaacattgca aatagtgcaa atccaattac aattacatct ttaaaattga
     1441 tttgtttctc acccatttaa agcaccccca tttgaatttt actacatttt actaaaaaat
     1501 aagtccaatc tcttcttaaa tgaaaacctt ttgacgagat tttatttaat tatacatttt
     1561 tgacttaaat ttgtcaatta tgtttcataa tttataaaat cttcacaata tttaatacaa
     1621 ctttcttaat taaacaaaat caacgtgtat actttaaaca tagttggttt tttgtttttt
     1681 atctaatttt tcttgataaa attaattatt ctgaataatt aaaatattag gattttttag
     1741 attattcgtt ttattattaa gttgtattgc tttatgtatt atatatcact ctccttttta
     1801 catacatata atctttttaa ttcaatttaa attttaaata ttttgtgttt attatataat
     1861 ctgaaaaaaa agcagtcaat agcattttta attttttaaa ttttcaaaat tatgattttt
     1921 gaaaaatcgt tttcctttta tcatagcacc tatttacgtt aattcttgta gtattttgta
     1981 tttattttgc taaatttata gttaacttta tttaatttct attctactta tatagattca
     2041 cctcttgctt tttagttttg aaatcgtttt ttctgaaaat tccttattat gatttatcaa
     2101 cttatttttc ttcatagtaa ttattttcta acttccaact tacttaaact ttgcagttgc
     2161 tagtattcac gtttacatac ttatctgtca taattaacta aaaattctta atatatctct
     2221 cagtaaaaac aattcaatca tttacttaat acatttatat aatcatatgt c
//
LOCUS       DAMJVM010000185         2266 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.190, whole genome shotgun sequence.
ACCESSION   DAMJVM010000185 DAMJVM010000000
VERSION     DAMJVM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.190"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(140..682)
                     /locus_tag="PC523_003457"
                     /pseudo
     CDS             complement(140..682)
                     /locus_tag="PC523_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            823..1065
                     /locus_tag="PC523_003458"
     CDS             823..1065
                     /locus_tag="PC523_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476936.1"
                     /translation="MNRIAYQTLTVSNSLSFLRPATPVSILKSSGVATHTLISTDKHS
                     NGYNFSYAAHSDEGVRTNLLSKLKGKKISYYKVYWE"
     gene            1430..1771
                     /locus_tag="PC523_003459"
     CDS             1430..1771
                     /locus_tag="PC523_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476937.1"
                     /translation="MLNKKNVSLIALVIILSLFTVIFVFNNRNTNDKLKEVTKNNEEI
                     VPKNLEDLSIKQLTEIRDKAIDKNLEGLREFGEDIVKKRKDLILNFEFGLVAFVNKNY
                     SNLTEKEKLIR"
     gene            1811..2155
                     /locus_tag="PC523_003460"
     CDS             1811..2155
                     /locus_tag="PC523_003460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476938.1"
                     /translation="MDPSGSFFELANQSYEINEFMLKNKKNYKEWSYEYIEFLIDHLE
                     ELCKFVDFDVKDVIDIIDPTIKTDLSDEQQKSLNDKLKKMSSSETLNEKIKKEIKNWE
                     NNLNSLNMNKNW"
BASE COUNT          920 a          265 c          276 g          805 t
ORIGIN      
        1 catatttttt gtactatttt ctataatatt ataacgaaat aacgtaaaaa caattattaa
       61 taaaattaca tttatagcac cagaaattaa gaatgccttt ttcttattat tcatatattc
      121 cctctctaga ttatctaaat tagtctatat caagcatttt taagtacttc tcattaactt
      181 tatatacatc cttttccctt gataaaagct ctatttcttc aagattatta aacacttcca
      241 tcaactggtc aattaagtct atttctttta ttccagcctt taagtatcta cttttattcc
      301 ttttaatata gttttccaat gtcatagaat agttatcttt actacagata tattttaata
      361 tatgttcttc tgttgctgat agagtaatgt cattttcaaa taactcttct ttcaacacac
      421 tctctaaaga attcctatca tgcatgtctc tcatttcatt tatagattgc attatggact
      481 ttagcatgaa ctctataaat acagttaaat tatatccctt attctctaca tcaagtatag
      541 ccttataata cttagttctg tttttagata tcatatatga tattgaaaac tctttaaaag
      601 tatcatatcc tttatcaatc agatatagat atgataatgc tcttgatgtt cttccattac
      661 catcactaaa aggatggata tggagattat gctaactttg tttcacaatg cttatatgag
      721 gctggagtaa cattctggac taataataat ccatggtatt attattctac tagtaataga
      781 tcaccttctt gggctggagc aagagagttt aaaatacatt gggtgaatcg aattgcatat
      841 caaacactta ctgtatcaaa ctctttaagt tttttaagac cagcaacacc tgtaagtata
      901 ttaaaaagta gtggagtagc aactcataca ctaatatcta cagataaaca ttcaaatgga
      961 tataattttt catacgctgc acattcagat gaaggcgtaa gaacaaactt gttaagtaaa
     1021 cttaaaggaa agaaaatttc ttattacaaa gtttattggg aataaagaaa agtttatgac
     1081 tttaatcaaa tgaaaattga aaagaataaa tatctatttc aaaaaatcaa taaaaattta
     1141 gcagaaacaa ctactaaatt ttattacata ataataaaat ttatttgtaa aaaagctata
     1201 taagtataaa aagaggacta atagaattta gtcccttttt atgatttaaa aatttaataa
     1261 aaaaattata tacatatgtc taaaaattag tttatcattc gttataatta tataaactat
     1321 atcaaaaaag tgttatattg tagtctttat aataagcagt aataaaggtt actctaataa
     1381 actgaattat tttttacaaa caaaatgctt attaaaaagg aggtttaata tgttaaataa
     1441 aaaaaatgta agtttaattg ctttagtaat aattttaagt ctatttactg ttatatttgt
     1501 tttcaataat agaaatacaa atgataaatt aaaagaagtt actaagaata atgaagaaat
     1561 agttcctaaa aacttagaag atttaagcat aaaacaatta acagaaatta gggataaggc
     1621 tattgataaa aacttagaag gacttagaga gtttggagaa gatatagtaa aaaaacgtaa
     1681 agatttaatt ttaaattttg aatttggatt agttgctttt gttaacaaga attatagtaa
     1741 cttaacagaa aaagagaaat taataagata aattaatatt gttaactatg aaagctctat
     1801 aaatgaaata atggatcctt caggttcatt ctttgaatta gcaaatcaaa gttatgaaat
     1861 taatgagttc atgttaaaaa acaaaaaaaa ttataaagaa tggtcatatg aatatataga
     1921 attcctaatt gatcatttag aagaattgtg caaatttgtt gatttcgatg taaaagatgt
     1981 aatagatatt attgatccta caataaaaac agatctttca gatgaacaac agaaatcctt
     2041 gaatgataaa ttaaagaaaa tgtcttcaag tgaaacttta aatgaaaaaa ttaaaaagga
     2101 gataaaaaat tgggaaaaca atttaaatag tttaaatatg aacaaaaatt ggtaatatct
     2161 caaaataagt ctggaaatat ttatgtgtat tctttaacta aaaatttatt ttttaataga
     2221 aataaagcaa gattaaaagt tgcaagactt caagaacata tagcta
//
LOCUS       DAMJVM010000186         2124 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.191, whole genome shotgun sequence.
ACCESSION   DAMJVM010000186 DAMJVM010000000
VERSION     DAMJVM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.191"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            48..>2124
                     /locus_tag="PC523_003461"
     rRNA            48..>2124
                     /locus_tag="PC523_003461"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          632 a          424 c          607 g          461 t
ORIGIN      
        1 aaaattatcg aagttgatag cttctaatct atcaaacctt tttaactggt caagttatta
       61 agggtgcagg gcggatgcct tggcactagg agccgatgaa ggacgtgata agctgcgata
      121 agcttcgggg agttgcacgt aaactttgat ccgaagattt ccgaatgagg aaactcactt
      181 agagtaatgt ctaagtatca ttaagtgaat acatagctta atgaggggaa ctcagggaac
      241 tgaaacatct aagtacctga aggaagagaa agaaattcga ttccgtaagt agcggcgagc
      301 gaacgcggat tagcccaaac caataaagtt ttctttattg gggttgcgga catatcataa
      361 cgaagaggta tcgtaattga agaggtttgg aaagacccac cacagaaggt aatagtcctg
      421 tatgttaaac gagaagactt cagatatgat ccagagtacc acgggacacg tgaaaccctg
      481 tgggaagcag gagggaccac cctccaaggc taaatactac ctagtgaccg atagcgtata
      541 gtaccgtgag ggaaaggtga aaagaacccc gggaggggag tgaaatagaa cctgaaaccc
      601 tgcacttaca agctgtggaa gcacatttct tgtgtgaccg cgtacttttt gtagaacggg
      661 ccaacgagtt acgttaagta gcaaggttaa gcacttaagg tgtggagccg tagcgaaagc
      721 gagttttaac tgagcgttca gttacttgac gtagacccga aaccgggcga cctacccatg
      781 agcaggatga agcgaaagta aaatttcgtg gaggtccgaa cccacgagcg ttgaaaagct
      841 cggggatgac ttgtgggtag cggtgaaatt ccaatcgagc ccggagatag ctggttctcc
      901 ccgaaatagc tttagggcta gcctcaaggt gagagatacg gaggtagagc actgaatgtc
      961 ctagggggta ttgcacctac cgaagactat caaactccga atgccgtcat cttatacttg
     1021 ggagtcagac tgtgggtgat aagattcata gtcgaaaggg caacagccca gatcgtcagc
     1081 taaggtccct aaatgtaagt taagtggtaa aggatgtggg attgcacaga caaccaggat
     1141 gttggcttag aagcagccac tcattcaaag agtgcgtaat agctcactgg tcgagtgatc
     1201 ctgcgccgaa gatttccggg gctaaaactt actaccgaag ctacggcatc agtaatgatg
     1261 ggtaggggag cttcccatac gggttgaagc atgaccgtaa ggacatgtgg acagtatggg
     1321 agtgagaatg ttggcatgag tagcgagatg tgggtgagaa tcccacaggc cgtaaaccca
     1381 aggtttccag gggaaggttc gtccgccctg ggttagtcgg gacctaagct gaggccgaaa
     1441 ggcgtaggtg atggacaaca ggttgatatt cctgtactac cgataaccgt ttgagagaag
     1501 ggatgacaca gtaggataag ctaagcacac tgttggttat gtgtgcccaa gcattgaggc
     1561 agtcaaagta ggcaaatccg ctttgataat gctgggatgt gatggggagc gaaatttagt
     1621 agcgaagtag ctgatttcac actgtcaaga aaagtctcta tcgaggttaa aggtacccgt
     1681 accgcaaacc gacacaggtg ggtgaggaga gtatcctaag gccagcgaga gaactgttgt
     1741 taaggaactc ggcaaaatga ccccgtaact tagggataag gggtgccacc atcaggtggc
     1801 cgcagagaat aggcccaagc gactgtttac caaaaacata ggtttctgct aagtcgcaag
     1861 acgatgtata ggagctgacg cctgcccggt gctggaaggt taaggggatc tgttagagca
     1921 atcgaagcag tgaacttaag ccccagtaaa cggcggccgt aactataacg gtcctaaggt
     1981 agcgaaattc cttgtcgggt aagttccgac ccgcacgaaa ggcgtaacga tttgggcact
     2041 gtctcaacaa cagactcggt gaaattgtaa ttccggtgaa gatgccggat acctgcgaca
     2101 ggacggaaag accccatgga gctt
//
LOCUS       DAMJVM010000187         2123 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.192, whole genome shotgun sequence.
ACCESSION   DAMJVM010000187 DAMJVM010000000
VERSION     DAMJVM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.192"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(763..1548)
                     /locus_tag="PC523_003462"
     CDS             complement(763..1548)
                     /locus_tag="PC523_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476939.1"
                     /translation="MKKQKIITSLLITGVLATNTYPVFASQAIQTQNLTSIEQRISSN
                     VLLKCDQSSINYTNFFKNGGGEQWVKLTLEGAKFNTNDPYEIKNAIKQNYDVQTTIAV
                     GTDGKSIEFGVYEPNAGIEYLPNWEININSSVLTSDTDATIKIPVNYDIHTSATPKII
                     TSKKSITLDELKNGFELDLEGIQGAQFNTDCVGNNVRLSLMHTANIPVMAMTYCDLDT
                     THIGFIIKALNDIPSTATEFTFRIEGNATTSQVPLLVKLPIVK"
     regulatory      complement(1903..1990)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          733 a          235 c          309 g          846 t
ORIGIN      
        1 aatttaaatt taataactat tagaataaat atttattccg aaaagatgct ttaaagtaaa
       61 ttacattttt atttataata agagatgcaa gtgtaatgtt ttagatataa taattacaag
      121 tatatataaa ttatactaat ggttttatag tagctaagtg tttatatggt aataaaaatt
      181 gtaattgtaa tttatgatgg tgataataca taaatgaggt tggcaatgat aagcttatgt
      241 agtttattaa ataaaaatta ttaataatca attatcaatg attattttaa cttatgcttt
      301 tgcattatat gtgtaaatat aacaattgaa gatatttggt atttgaaatt tctagtttta
      361 tgagaaacat aatatcgctc agataaattt gttatgacat tacaagaatt tttaaaggta
      421 tattgcttaa acttaaaggc aaagatatat tgtcagataa agcagtatct attaaagatg
      481 aaatttttat taaagagcaa gtagaaactg tcatatatct tcaaatttag taagtattat
      541 tttgtgaata tctttgcctt tattaatttg tattgattgt ttttcaatta aatcttctat
      601 tatagttgat tatatgaaaa attctgtatt atttagtaat cataatatag tttagtttct
      661 ctgcttattg tataagtttt taaagcagat gtaatgtagc ttagttatag tcatagggat
      721 aacagtatta gcttttaaag tttatattat atgtttaaag ttttatttta caataggaag
      781 cttcactagt aatggtactt gagaagttgt agcatttcct tctattctga atgtaaattc
      841 tgttgctgtt gaaggaatat catttaaagc ttttattatg aaaccaatgt gtgttgtatc
      901 aaggtcacag tatgtcatgg ccattacagg aatattagca gtatgcatta agctaaggcg
      961 gacgttgtta cctacacaat ctgtattaaa ttgagcacct tgtatacctt caagatctaa
     1021 ttcgaagcca ttttttaatt catctaatgt tatacttttt ttacttgtaa taattttagg
     1081 tgtagctgat gtatgaatat catagtttac aggtatttta atagttgcat cagtatctga
     1141 tgttagaaca gaagaattaa tgttaatttc ccagtttggt aagtattcaa taccagcatt
     1201 aggttcatat actccaaatt caatggattt tccatctgta cctacagcta ttgttgtttg
     1261 tacatcatag ttttgtttta ttgcattttt aatttcatat ggatcatttg tgttgaactt
     1321 tgcaccttca agggtaagct ttacccattg ttctccacca ccatttttaa aaaagttcgt
     1381 gtagttaatt gatgactggt cgcatttaag aagaacattt gaagatattc gttgttctat
     1441 tgaagttaaa ttttgtgttt ggattgcttg agatgcaaac actgggtaag tatttgtagc
     1501 taatactcct gtaattagta gtgatgtgat aattttttgc tttttcattt ttgtttcctc
     1561 ctataatatg ttatattttt aacagtgaaa tgaaataaaa tacaagggca atataaaata
     1621 tatgtcgttt tgaagtgtat taagattgtg tgaaatatta ttagttatac tgaaatatac
     1681 tagtctatag atatacatag tttaaaataa gtaaagtagt attactgtct tacttaacac
     1741 tcgtttttaa tccataaaat aatgttttca gagttaaata attatctttc aaatctaatc
     1801 tcaattcttt agcatagagg ttatagatac aataataaga atatggctag aacaaaaaaa
     1861 tacctcataa ggaggtatta tctaacaaaa agtacactaa cttttagtaa ctggcaggca
     1921 taatcttttt aagaccttaa cccctatagc tttgcgtccc tagatttctc taggtttgcc
     1981 tattaagttt tgcgtttgca tttttaatat catatcaatc tgttttatag aatgataaca
     2041 caaataaaac ttataatcaa caattacatg aaatttttgg aaaaaaatta tatattacaa
     2101 aaataatatt ttcttcatga ata
//
LOCUS       DAMJVM010000188         2093 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.193, whole genome shotgun sequence.
ACCESSION   DAMJVM010000188 DAMJVM010000000
VERSION     DAMJVM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.193"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            385..1221
                     /locus_tag="PC523_003463"
                     /pseudo
     CDS             385..1221
                     /locus_tag="PC523_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            complement(1313..1780)
                     /locus_tag="PC523_003464"
     CDS             complement(1313..1780)
                     /locus_tag="PC523_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2476940.1"
                     /translation="MKFGSVLIVVEDIEKSKRFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKGDSEVKFNGNDAELYFEEDNFDTFVERLSTMKDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSMDEVAKRMDVTVEYIESVLD"
BASE COUNT          858 a          298 c          220 g          717 t
ORIGIN      
        1 tatttataaa attatattat caatagaatt tattaaaata aatttttttg ggtgtatatt
       61 ataatttaat cgaatagaaa tgaaaaatat acatacagtt tagattttag gcaacaatta
      121 gggggatttg gtgacaagat ttaattataa gaaggaaagt gtcttaatgc aacttttgta
      181 aggtcattat agacactttc ttttttattt gataaattta aattatcaaa attagattgc
      241 tttatacaac tattttgttg gaaactattt attatataag gagaaatcaa taaaaaaatc
      301 agtgcttctt ataaatgata atagaaaaga tttaacagat tttttagaaa ataattttaa
      361 attagttttt ggagattata taaattaaaa aatttagaac aaaatctcac taaaaaatta
      421 aaagaaaaag gacaaatagc aaaatataca tttaaggata taaaaacaac tagccccaaa
      481 atgctcgaat atatagattt aactaagaag gtttctaaat ctgacttaag catacttgtc
      541 agaggtgaaa gtagaaccgg taaaaaactc atagctcagt ctatacataa caactcaaat
      601 agaaaaaatc aaccctttat agcagtaaat tgtacaactg ttcctgaaaa tttattagaa
      661 aatgagatat tcggatatga caaaagaact tttactggag gtctaaaaga tggaaaataa
      721 ggattatttt agcctgctaa taatggaata atattcttag atgaaatagg ctatatgccc
      781 ttagaattac aaacaaaatt acttaaagtt cttcaagaaa aacagataat gcctgttggt
      841 tctcacaata taattaatat aggtgttaga attatatctg ctacaaataa gaatttggaa
      901 caaattattg ataatagcca ttttagagag aatttatatt atcgtttaaa tacaattcca
      961 ataaatatac ctcttgtaag agaaagaaga tatattaatt attatggaag atttaattaa
     1021 taagaaatta gtaatcacac cagaagcaaa aaaacttata caaaattata cgtggaagga
     1081 taatataaga gaacttcaaa atgttacatc ttatttaaat attatgtgtg aagatattgt
     1141 attagagaaa gacttacctc caaacttaag gtcttcaagt aataaaaccc atcattaaaa
     1201 ttaaaatata gtaagaatga tatattttac aaaaacaaaa actgtaagta aatttatttt
     1261 cacacaagtt tacttacagc cctcacctca gttttatttt agcataatcc atctagtcta
     1321 aaactgattc aatatattca acagttacat ccattctttt agctacctca tccattgaaa
     1381 gaccactatc taaaaaacgt ctacacacag atgacatagt ttctccaacc tcaataatat
     1441 gcccatctaa atcataaaat ctaattgctc tttgacccca cctatgctct atagctagat
     1501 gaacataatc tatatctttc attgtactca atctttcaac aaaagtatca aaattatctt
     1561 cctcaaaata taactcagca tcatttccat tgaacttaac ttcactgtca cccttattaa
     1621 taaactctaa ccaaagcttt ttttcttgta atgatatatt cccttctaat acaacatttt
     1681 ctccaaaatc acagattacc tttaatccca aaacatcata ataaaacctt tttgattttt
     1741 caatatcttc aaccactatt aaaacacttc caaacttcat atttcctcct tgatatacac
     1801 atattttatt ttcattacaa aagtaaaatt aaactataca tatttcaata agacatgata
     1861 taaacaagaa aattgtaagt cttatttaga tagttgagtt gattatatca tataatattt
     1921 gctttactct tgtaaaaaac agacatctct acacttttag attcaaaatt aaaatacata
     1981 taaatttaac taaaaaactt cctgtttttg tagattatat tattatctaa cttctaaaac
     2041 aaaaatacta ttttagaata ttgaaatatc tatattcttt taatttcaag taa
//
LOCUS       DAMJVM010000189         2089 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.194, whole genome shotgun sequence.
ACCESSION   DAMJVM010000189 DAMJVM010000000
VERSION     DAMJVM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.194"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(<1..332)
                     /locus_tag="PC523_003465"
     CDS             complement(<1..332)
                     /locus_tag="PC523_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2476941.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMGFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDANSIILKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQG"
     gene            complement(818..>2089)
                     /locus_tag="PC523_003466"
     CDS             complement(818..>2089)
                     /locus_tag="PC523_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2476942.1"
                     /translation="SISKTTANQIKSTVNASQTRLNGSNRYETSLLIAKEIDKNHDVE
                     KVYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTYKWLKSEDLQSAYFIGGP
                     QMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFYTDDELEAVLVAKSDVLV
                     DALAAGPLAANLKSPILITPKTYVSAYHKENLEAKSANKVYKIGGGLTSKVMSSIASS
                     LSKHNTTPTDPGTSGGKTVMIDPGHGGSDTGTTGKPLGGIKEKDYTLNTSLATTEYLR
                     SKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNASDTTGNGVEVFYKLKDKD
                     GGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLYVLRNNDMPAVLVECAFL
                     DNEKDMSLLNTSDKVKEMGTQIGKGIEDSLK"
BASE COUNT          638 a          350 c          251 g          850 t
ORIGIN      
        1 ccttgtagtt cccaaacatt tagatattct gggtctttat tataattaat agttccctca
       61 tacgactcag ttgattgatt gtagtttaga gttgtgtcta atggcatatc ataacattta
      121 tattttaata taatgctatt tgcatctggt ttttctttaa atttgacgct aacttttgat
      181 ttactaccta aaacgatact ttttttctct aagtttatat ttaaatcagc attattttcc
      241 atagcattag attcagtagc aaatcccatt gtaggtgcaa cgattgccat tgaaagaatc
      301 cctgttgtta ataattttgt atttcttctc atattaatct ccttatttaa cataaatttc
      361 tactttgttt tttaaccttt taacttttga aaatatggca aagtgtatct atctaaaaat
      421 ataaataagt acataaagat aaacttataa aactcaaaaa ataaaaaaat taaatatatt
      481 taatacattt aattatttat actatagcat ttattaccat aaaaatccac aaaaaaacac
      541 aataaagtat agtaattgaa cttttactgt gtatcacaaa tgaatacgcc aactcttgac
      601 gattagctat aaccttaaaa tatgtaaatt ctttatataa cataaattct tttacaatta
      661 actacaaaaa gactatatat acatcactta gcacttatat gcacaatgta tatatagtct
      721 ttattactat tgtaattaca ttcttatctt atagctttag ttaaaattta aaattgcaaa
      781 ttaatttatt ttaaattttc tatgctctca ttgttggcta ttttaatgaa tcttctattc
      841 ctttacctat ttgtgtaccc atttctttta ctttatctga tgtattaagt aaactcatat
      901 ctttctcatt atctaaaaat gcacattcta caagtacagc tggcatatca ttatttctta
      961 aaacatataa ataatctttt gtagggtcag ttgatagtgt tcttgttttt gcacctctat
     1021 tttttagatt aaatttctct aaaattctat ttaatatatt tgtagctaca gtcttagtag
     1081 ttcctccatc tttgtcctta agcttataaa atacttctac accattccca gttgtatcag
     1141 atgcattata gtgtatactt gtaaataaat ctggtctcaa agagttagat agagcagttc
     1201 tatttccaag agataaagtt ttatctgtgt ctcttgtcat tattacattg aacccctttg
     1261 aacgtaaata ttcagttgtc gcaagtgaag tatttaaggt atagtctttt tctttaatac
     1321 ctcctaatgg tttacctgtt gttccagtat ccgaaccgcc atgccctggg tcaatcataa
     1381 ctgtcttccc accactagtt cctgggtctg ttggagttgt attgtgttta gataatgatg
     1441 atgctataga actcattacc ttagaagtca atcctcctcc tattttatat actttattgg
     1501 ctgatttagc ttctaagttt tctttatggt atgcagatac atacgtcttt ggtgttataa
     1561 gtattggaga ttttaagtta gcagccaatg gacctgctgc taaagcatca acaagtacat
     1621 ctgatttagc tactaaaaca gcttccaact catcatctgt atagaatttt tttattacat
     1681 ttgcatttgt ttcgtgtcta tctgctccgt atactctatt attagtaaca ctatccttag
     1741 ttattccatt taccttattt ataacatttg ttgatatcat ttgaggacca cctataaaat
     1801 aagcactttg taaatcctca ctctttaacc atttatacgt attatctgta atactatctt
     1861 tatcagttag tattattggt tgcttatctt gacctgcttt tgctgctatc gtaagtgcat
     1921 ctacttctcc accattagca tttgttatgt atactttttc cacatcatga tttttatcta
     1981 tttcctttgc tatcaataaa gatgtctcat atctattaga gccatttaga cgtgtttgac
     2041 tagcatttac agtcgattta atctgattag cagtagtttt agaaataga
//
LOCUS       DAMJVM010000190         2078 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.195, whole genome shotgun sequence.
ACCESSION   DAMJVM010000190 DAMJVM010000000
VERSION     DAMJVM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.195"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(<1..240)
                     /locus_tag="PC523_003467"
     CDS             complement(<1..240)
                     /locus_tag="PC523_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476943.1"
                     /translation="MSNNLSNININENNLIKNQYSISLIKECFDCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYA"
     gene            complement(263..697)
                     /locus_tag="PC523_003468"
     CDS             complement(263..697)
                     /locus_tag="PC523_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476944.1"
                     /translation="MISPIILSSINRNLKEIERNKLFETNIESRDYGLTLSESDVKDI
                     INFRDNTLKGYGRIELDIKVTKQLIENIYISQYTNMDNYLETINDMQEIFYYLKNETD
                     DKICDDEIIEILDELYEKFSGNINNVRGEADEFVKKFKFGEV"
     gene            complement(957..1868)
                     /locus_tag="PC523_003469"
     CDS             complement(957..1868)
                     /locus_tag="PC523_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HDF2476945.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYRITEEDELYIVPTEDAVFSMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLDFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSADEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLIFSKFYTEQLDWIVSFAKDMYKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
BASE COUNT          680 a          342 c          163 g          893 t
ORIGIN      
        1 agcataccat atcgaaatca acaatttttc agcaacttct gtttttactg atgtgctttg
       61 accatttgta tattttttaa tcaaatccat aagtattagg gatatttctt gttgtatatt
      121 atatacttct ctttcatcta taactttaca atcaaaacat tccttaatta aagatatgga
      181 atactgattc tttattaaat tattttcgtt tatattaata ttacttagat tatttgacat
      241 atcaaatctc tcccatcata cattaaactt ctccaaattt aaatttcttt acaaattcat
      301 ctgcttctcc tcttacattg tttatattgc ctgaaaactt ttcataaagt tcatccagta
      361 tttctataat ctcatcatca catatcttat catcagtttc attttttaaa taataaaaaa
      421 tttcttgcat atcattaata gtctctaaat aattgtccat atttgtatac tgtgatatat
      481 atatattttc tattaactgt tttgtaactt ttatatctaa ttctattctt ccatacccct
      541 ttaaagtatt atctctaaaa tttattatat cttttacatc agattcagaa agagttagac
      601 cataatctct agattctata ttagtctcaa acagtttatt tctttctatt tcctttaaat
      661 tccgatttat agaactcaaa ataataggtg atatcatttt taaactctcc ttttttattt
      721 tttctattga ctttttttat tttttatgtt atatttaaat taatatacat tcttttagca
      781 actacttata atagtattat tgtaagtagt ttttgtcaac tcaaaattac tatctcaatt
      841 aaacttttcc attttctcaa taaaaaaata ccattattta gatatataat tttctataac
      901 aacttttaat tcagcagtta tttactaaaa tctaaacaat gatatattta ttaattttat
      961 ttatcttttt tagctctact tctacttttc tttacatttg aacaatttct acaagaaggt
     1021 gaacagtatt gagatttaga atttgtagga atataaattt taccacagta cttacattta
     1081 tttaaagaag aaggttcact tgtaattgca aaagcataga tagtctcaat cgctgttttc
     1141 aaagaatcaa actcccatgc aataattggt ttatctaatt gacttataga aaatcctatc
     1201 ttttggggat taaaccctcc agacataata gtaacacttt cagttaacag attagaggta
     1261 tttttatata cacaaatttg attaaagtgc ttatacatat ctttagcaaa gcttacaatc
     1321 caatctagtt gttcagtgta gaactttgaa aatattaaat ctaaaatcaa tggtctcttt
     1381 ccaaaaaact ttggagattc ttctctcttt gatacatcta tacctcttct atacttttta
     1441 aaatacacgt cttcatcact tgcaaatggt ataaacatgt ttacatattc atcagcactc
     1501 aatatatttt cttttgtaat ataattcttt tcaatcatca gtactctttc ttctccaact
     1561 atatctctat tatatacact tgagtatatt aaacccaata atccatattt ttttacaaag
     1621 tcaagtactt ctttctttaa tttcatttct ttcgtctctt ttgatatatc ttgtagtgcc
     1681 tcttgtccta tacgaattat atctactatt atatcatctg ctacatcaaa tgggttgtac
     1741 atgctaaaca cagcatcttc tgttggaact atatatagtt catcttcttc agtaattcta
     1801 tattcataat cactatacct aatccaatca cttgcaaaat tccctattaa ttctattttt
     1861 ttattcatga taatcattta cccctttcaa tgtaatatat aattttatat tactattaca
     1921 tttttagttt attccatatt ttctatactg tcaactattc ttttatattt ctgtaatatc
     1981 aaaatttata acaaccttta taatgaactt actaagctaa atttcttgtt tattaataac
     2041 tagtccataa ttaaatttat aaaaactatt tacatata
//
LOCUS       DAMJVM010000191         1988 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.196, whole genome shotgun sequence.
ACCESSION   DAMJVM010000191 DAMJVM010000000
VERSION     DAMJVM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.196"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(59..1276)
                     /locus_tag="PC523_003470"
     CDS             complement(59..1276)
                     /locus_tag="PC523_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HDF2476946.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWMK
                     DLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNKSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TNFGFNTKNLTNLHPRKDIDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTADLVATVGKMNFYPSSSNVVPEKA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(1468..1881)
                     /locus_tag="PC523_003471"
     CDS             complement(1468..1881)
                     /locus_tag="PC523_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2476947.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYFRKIL"
BASE COUNT          680 a          316 c          232 g          760 t
ORIGIN      
        1 tataagtata aagtgagttt ttagattata tattttaata tactttctta tataaaaatt
       61 attttaaatc aagattatct agtattttta aaaaaatttt tacagcggta tccaaatcat
      121 ctacagaaac actttcatta ggattatgac ttacgccccc agcgcatctt ataaatacca
      181 taccaatatc agttatatta tccatttcct gagcatcatg accagcacca ctataaatat
      241 aaaaaggatt aagatttaaa tctataaaac ttttttctat gatttttgtt attttattag
      301 aacatggtac aggaacattt tcaaaagcaa gttcacttgt atagttaagt tttctatttt
      361 cacatatatg tgatatttca ttaaatatct tttctacaga attatcaaga atttcttgag
      421 aacaagagcg tacatcaaga gtaaattcag ctttttcagg taccacattt gatgaagatg
      481 gataaaaatt catctttcct acagtagcaa ccaaatcagc agttgtctta gctacttttt
      541 cgatagctaa gacacattca caggcacaac atccagcatc aagtctcatg ttcattggga
      601 gtgtaccagc atgtccagat ttaccattta ctgatatctt aaaacttttg aatcctgtaa
      661 tacttgatac aattccagca ggaagagatt cattttctaa gacaggtcct tgctcgatat
      721 gaaattctaa atatgcatct atatcttttc taggatgcaa atttgttaag ttttttgtat
      781 taaatccaaa atttgttaca gcttcttcaa gtgtaattcc attttcatca acagatttaa
      841 gaagttcctc tttaaaagta ccagcaaaaa ctttacttcc cattaaagaa gtttcaaatc
      901 tagttccttc ttcttcagcg aaagcaatta gtttaatatt gaaaggataa gacttattat
      961 tgtgttttgc ttcttcaaga ccaactagag gcactattat gcctaacata ccatcaaata
     1021 tacctccaca ctttacacta tcttgatgag atgcaactac taatgtggga gcatttggaa
     1081 agcttgattt atatgtacct ataacatttc caatgtcatc aatttcaata tcaagattta
     1141 aatctttcat ccatgaactg attaaatcaa tagatttttt atgttcttga gtcaagataa
     1201 gtcttgtaag accatttgtt gttgaactta tttttcctaa tgtttgtaga taatccatac
     1261 atttttttcc taacatattt acctcctaaa aatataattt ttatttaagc taagattagc
     1321 ttttatactt ataatttttg aactttcaaa taattttgta tgtatagtaa aatactattt
     1381 tagtattaat aatgatataa gaacataaag tctgtagacg aagagcagaa gttttaagtt
     1441 aaaaaatatt aaatgtgatt tttattatta tagtattttt ctaaaataat atctctttcc
     1501 agttagagga taattttcaa gtgaaaatac tttatggtat ccaaatttct cataaaattt
     1561 aggtgcttga aaactgaatg tatctaaaaa aacatattta caacctcgtt ctttagcaat
     1621 tttttcagcc ttatataaaa gttgacttcc agtaccttta tatctaagct tctcactaac
     1681 ccataagtat tttatagaaa gccaattacc atgagtaaca cctataaaac ctgcaacaat
     1741 tttacctgct ttatctcgaa tatatattcc taaatccact ggatttttat cttcaagtct
     1801 agcaagatta tattttaata atccttgaaa aatagtatct tcatcttgtt tttctatttt
     1861 ttctgtaatt tctaattcca tatttgaact ttctccttgt ataatctttt gcatattggt
     1921 attttttaat aaaatcagta gaaagaaaat agtttaacat tattctaata gtcatttatg
     1981 ctaatgtc
//
LOCUS       DAMJVM010000192         1864 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.197, whole genome shotgun sequence.
ACCESSION   DAMJVM010000192 DAMJVM010000000
VERSION     DAMJVM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.197"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            67..324
                     /locus_tag="PC523_003472"
     CDS             67..324
                     /locus_tag="PC523_003472"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476948.1"
                     /translation="MHNVDCNDKSVYIGNNELDRSKYLKENELLALFGDGASIDMKHN
                     SKLDMQISNPNLKSVNIYPYTPFVGLRGTLYIIEKILNIQY"
     gene            556..747
                     /locus_tag="PC523_003473"
     CDS             556..747
                     /locus_tag="PC523_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476949.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTKPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            complement(835..1647)
                     /locus_tag="PC523_003474"
     CDS             complement(835..1647)
                     /locus_tag="PC523_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HDF2476950.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNRVTEISDDLDSILTNVVEKIK"
BASE COUNT          728 a          242 c          240 g          654 t
ORIGIN      
        1 tatgcaaaat tctatgaagc aggaaccctg tgactttagt catgggaggt tcagagagtt
       61 aatgtattgc ataatgtaga ttgtaatgat aaatctgtgt atattgggaa caatgaactg
      121 gatagaagta aatatttaaa agaaaatgag ttattagcat tatttggaga tggtgctagt
      181 atcgatatga aacataattc aaagttggat atgcagataa gtaatccaaa cctaaaaagt
      241 gtaaatatat atccatacac tccttttgta ggattaagag gaactcttta tataattgaa
      301 aaaatactaa atatacagta ctaaatatat aaaaaatatt atttaaatac atatacaata
      361 cataaaaaag tttttattaa gaaaatgttg ataaaaaaaa ggtacattta ttaaaaaatc
      421 aatctctact tgctaaaata attatgaatt atgttagaaa ctgtaatata ttggatataa
      481 tataataaac gggtaaactt tggattaaga ggattatata gtttatctta attatagggg
      541 aagtgaggat atattatgga aagattaatg gaactcatag aaagaaaatt tttaaaggat
      601 gtagaacttt tagaattaga agacatagaa aatgtaatag aatgcaaaat gactaagcca
      661 tataatgctc attataatga atacttagta aaagtaaaaa atggagatga agtagaagaa
      721 tattttgtgt atttaaaaca tagataaaca taaaagaggt ttatatttat ttgtatgatt
      781 ataaaataaa tataagcctc ttttttgata aatataaaat tataaaattt ttatttattt
      841 aattttttca acaacattag tcaatattga atctaaatca tcactaattt ctgtaactct
      901 attatgagta atatctataa tttctttagt cataggaagt tctcctaaaa gctctaagtc
      961 taaatcatct aaaaactttt cagtagattc tccttcaaat aacttgattt ttttagaaca
     1021 atcagggcat tggatataac tcatattttc aataactcca aggacattta tgttcatttt
     1081 tttagccata ttaacagctt ttgatacaat catagatacc aaatcttgag gtactgaaac
     1141 cataactatt ccacttatag gaatagattg cataactgtt aaggcaacat ctccagtacc
     1201 aggaggcata tctattaata aatagtctaa ttcttcccaa agtacatcag tataaaattg
     1261 ctttacaact cctccaagta aaggtcctct ccaaacaaca ggttcattct catcatctat
     1321 cataagattt atagacatta cttttatgtt atttgagttt tctacagggt aaatataaga
     1381 gccatcagaa taagctttta cattttttac acccataagt ctaggaatac ttggtccagt
     1441 tatatctgaa tctaatatac caaccttata tcccattttg tttagcttat tagcaagtaa
     1501 agcagttaca gttgattttc caactccacc cttcccactc ataacaccaa ttatttttcc
     1561 aaatttattg ttagggttat tttcaataga acaatttgat tgactattac aatttccctt
     1621 tgatggacat gaattacaat ctgccatatc tattacctcc aaactttaat atttattaga
     1681 ttatttgtaa cctacatata ctattttagt acaattgaac cataaagtca ttatttaaaa
     1741 gtaacttaga agattttcta ttaatttatt catagactat agcaaaaaga atacaataaa
     1801 aaaattataa cattcttgaa aaagtgatta tttcaattta gttatagaac ttagtcatat
     1861 aaat
//
LOCUS       DAMJVM010000193         1843 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.198, whole genome shotgun sequence.
ACCESSION   DAMJVM010000193 DAMJVM010000000
VERSION     DAMJVM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.198"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            <1..1259
                     /locus_tag="PC523_003475"
     CDS             <1..1259
                     /locus_tag="PC523_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476951.1"
                     /translation="YINKLGDIEKRVVELTAKNVKKVYLRGIEILEEELVRLKNFYEL
                     MMSRKLDTNSIAYNDTLMELSSFFKSYIIKFEAQDIPVSIDYPLALDDMDIQGIYYVK
                     NYIESIDIENRFCNLFKKKDREKLFYDYGVTYKIDCDVILVNLFELIINNCIFSTILG
                     NEVTNLEISEYEYEFLEKQFKNICNNIAEDKVIVSEESNIIELIEVDDSFNTEADSNL
                     DYNDFEKITLILMEAIEVLVKRLRIEDEVVIRYIKKYEKRFIESVLSSIKSNTFKSMV
                     TVSKNKNEQLESYIVDDESKLDDESFRKIFNEILDSTSIIEKIRIIKENINAKKDFID
                     ILESECLFGEEYLMLFASLSELELAILGSVVFYEDIRMREINILEFLLNKKTETTLWK
                     IEYLKFMKRQSEDKIRSIEEHMNKIN"
     gene            1343..>1843
                     /locus_tag="PC523_003476"
     CDS             1343..>1843
                     /locus_tag="PC523_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2476952.1"
                     /translation="MKEHIINNIEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGESGH
                     GCGHNLLGVAGVGAVVSTKKLIEENKFSGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHP"
BASE COUNT          769 a          130 c          353 g          591 t
ORIGIN      
        1 cctatataaa taagcttgga gatatagaaa aaagagttgt ggagttgact gctaagaatg
       61 ttaaaaaagt gtatcttaga ggtattgaaa tcttagagga agaattagtt agactaaaaa
      121 atttttatga actaatgatg agtagaaaat tggatactaa ttcaatagcg tataatgata
      181 ccttgatgga gctatcaagt ttctttaaaa gttatattat aaaatttgaa gctcaggata
      241 ttccagtaag tatagattat ccattggcat tagatgatat ggatattcaa ggaatttatt
      301 atgtaaagaa ttatattgag agtatagata tagaaaatag attttgcaac ttatttaaga
      361 aaaaagatag agaaaaatta ttttatgatt atggggtgac ttataaaata gattgtgatg
      421 ttatacttgt aaatttattt gaacttatta taaataattg tattttttct acgattttag
      481 gaaatgaagt gactaattta gaaatttcag aatatgagta tgaattttta gaaaagcaat
      541 ttaaaaatat atgcaataat attgctgaag ataaagttat agtatctgaa gaaagtaata
      601 taattgaatt gattgaagta gatgatagtt ttaatactga agcagatagt aatttggatt
      661 ataatgattt tgaaaaaata accttaatac ttatggaagc aattgaagtt ttggtaaaaa
      721 gattaagaat agaagatgaa gttgtaataa gatatattaa aaaatatgaa aaaagattta
      781 ttgaatctgt gctaagttca ataaaaagta atacttttaa aagtatggta actgtaagta
      841 aaaataaaaa tgaacaatta gaaagttata ttgtcgatga tgaaagtaaa ttggatgatg
      901 agtcgtttag aaagatattc aatgagatat tagacagtac gtctataatt gaaaaaataa
      961 gaataataaa ggaaaatatc aatgctaaaa aagattttat cgatatcctc gaatctgaat
     1021 gtttatttgg agaagagtat ttaatgttgt ttgcttcatt aagtgaatta gaattagcaa
     1081 tattgggtag tgtagtgttt tatgaagata ttagaatgag agaaattaat atattagaat
     1141 ttctattaaa taagaaaact gaaactactt tatggaaaat agagtacctt aaatttatga
     1201 agagacaaag tgaagataag atacgctcca tagaagaaca tatgaataaa attaattaac
     1261 aaaaaattgt gaataaatag aatgttagta tataatataa tttatgaagc atatcaaaaa
     1321 tacatatatt tggaggaaaa ttatgaaaga gcatatcata aataatattg aagaaataag
     1381 agaagaatta atagatttat ctaagaaaat atgggaaaat ccagaattag cttttgaaga
     1441 aaagtatgca agtagcatcc aaaaagaata cttaaagtca aaaggattta aaattgaaga
     1501 agtagaaaat ttacctactg gatttatagc aagttttgga gagggtaaac ctgtgattgg
     1561 catacttgga gaatatgatg ctttaccaga gctatcacaa tgtgtgagtg cagaaagaaa
     1621 gccattggta gaaggagaat ctggacatgg atgtgggcat aacctattgg gtgtagcggg
     1681 tgtaggagct gttgtttcta caaaaaaatt aatagaagaa aataaattta gtggaacaat
     1741 aaaatatttt ggttgtcctg ctgaagaaga aggtggagga aagacagtta tgtgtataaa
     1801 tggatgtttt gatgatgtgg attgtgcatt tacatggcat cct
//
LOCUS       DAMJVM010000194         1833 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.199, whole genome shotgun sequence.
ACCESSION   DAMJVM010000194 DAMJVM010000000
VERSION     DAMJVM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.199"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(340..1668)
                     /locus_tag="PC523_003477"
     CDS             complement(340..1668)
                     /locus_tag="PC523_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2476953.1"
                     /translation="MESNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMIVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPASELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYSIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGIKR"
BASE COUNT          664 a          335 c          202 g          632 t
ORIGIN      
        1 ctatataata caatattcta ttatatacaa cagaaagatt taattttcat aaaacctata
       61 tataatattt gttatataga tatcatttag tttgataaaa aaatacttta aaataaagta
      121 ttttaaagca aataattttt tagatgttta attttgtaat ccaaaaacta aaaataaaat
      181 aaatacctta agataagctt tttagttcat gaaacactct ttttttataa aatcaaacct
      241 atctttgtca ttttaacaat aaaaaagaga cttctctcta ttttgagaca gtctctttta
      301 ttttttaaat aattataaag catttgtaaa ctacttaatt tatcttttta ttcctctagt
      361 catataagca tacactggta aacctatagc tgttattaca agtcctccta tagatatcat
      421 agtagttttc atacctgcaa aacacaattg attaactaca acaaatatac cacttgctat
      481 agctattatc ggaacaatag gatataatgg cactttatac tctcttggta tatttggatg
      541 agtctttcta agtttcatta cccctataaa agttaataca tagaatatcc aagtagcaaa
      601 tacagctaag tcagttaata aattaaattg acctgacaaa gcatatatag atgcaataac
      661 agatactaat gctattgcat ttgctggaac ttgagcagaa tttaatttag agaatatact
      721 atattttggt aaagtcttat ctgtagctaa agtgtatgcc acccttgaac cagttagtaa
      781 aaatccattt aatgctccaa acactgaaat taatatacct acagatatta ttttcccacc
      841 cattgaacca aatataactt cagcaactgc tgatgcagga gaagcataat ttgctaattc
      901 actagccggt aatacccaaa gatatgctaa atttattata aagtaaacag ccataacaac
      961 tgataatcca cctattatag cttttggaag gtcttttcca ggattcttca tttctccagc
     1021 caaagtacca acattcatcc atccatcaaa tgcaaataat atagcaatta aaacttgacc
     1081 taatacacta cctagactta atccctcacc aaccataggc gtaagtattg gattatttcc
     1141 accaccttta ataaatccaa caatcattat taaaataagt ggtattaatt tacaaattgt
     1201 agaaacagtt tgtataactc ctccagtttt tgaacccagc atattaagcc ctgcaactat
     1261 tactataatt ccaattgcta ttggaagtaa gagtgatgga ctaccaatta gtgctgatgc
     1321 ttgttctccg aatataacac ctaatgctgc catcatccct ggataaaata atacaatttg
     1381 catccatcca gttaaaaaac ctaatttttc tccataaatt tcttttatat ataccatcat
     1441 tccaccagtt tttggtatcg caactgatac ctctgcagct gtaagaccag ctgttatagt
     1501 tatgattcca gctattaacc atgccaatat accaagacct ggagctcctc cagttaatgt
     1561 ataaactgct tgtggtttaa aaaatacacc cgctcctata acagaaccaa caacagttga
     1621 taatgccgct gatacgccaa gagtcttttt cagttcatta ctttccattt tcttcctcct
     1681 cattaatcct atttaatttt aatttcatag ttaaataagc atatattatg ccaatactat
     1741 agaaaaatag ctttattatt tattaaaata aaataaactc tacttagctt ttgtaatttt
     1801 ttgcttttaa tcaagttcaa atataaaaaa att
//
LOCUS       DAMJVM010000195         1778 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.200, whole genome shotgun sequence.
ACCESSION   DAMJVM010000195 DAMJVM010000000
VERSION     DAMJVM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.200"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            142..1191
                     /locus_tag="PC523_003478"
     CDS             142..1191
                     /locus_tag="PC523_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2476954.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWVQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
     gene            complement(1340..1687)
                     /locus_tag="PC523_003479"
     CDS             complement(1340..1687)
                     /locus_tag="PC523_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2476955.1"
                     /translation="MHENKKNLLDGSLAQYNDVAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
BASE COUNT          660 a          247 c          277 g          594 t
ORIGIN      
        1 tattaaaaag actaaacata aaactgaaac ttattattaa gtttttgtag aaatattata
       61 agtatagata catttttgta acaactacaa tgaatattta atactatata ctattcgtat
      121 gtcttatggg aggtatttat tatgtttaaa aaaatagtcg caatagcaag ctctatcatt
      181 gtaagtacaa tatgtctaac tggatgtgat tttaactcta atgacaaaaa agatgaatct
      241 tcttcgtcta aaaatctgga taaagtaact atagcagaag ttactcactc tgttttttat
      301 gctccacagt atgctgctat tacgaaagga ttctttgaag atgaaggtat aaagattgac
      361 cttataaata cccaaggtgc agataaaact atggcagctt taatttcagg tgaagcaaac
      421 gtaggtttaa tgggccctga agcatctata tatgtatata atcaaaacaa aaacgattat
      481 gcagtgaact ttgctcaatt gacaaaaaga gatggtagtt ttctagttgc taggaaaaaa
      541 atgcctaatt tctcttataa tgatttaaaa ggaacagaaa tattaggtgg tagaaaaggt
      601 ggagttccac taatgacttt tgaatatgtt ctaaaagaaa aaggtcttac tattggagaa
      661 aacctaaagg caggcaatgt taatgtaaga actgatgtac aatttggtgt aatggctgga
      721 gcttttgcag gtggagaggg ggactttaca acagcatttg aacctactgc aacagcaatg
      781 gaaaaagaag gtagtggata tatagttaca tctataggaa aagattctgg agaaatacct
      841 tttacagcat actctactac aagatcatac atgaaaaaca ataaagattt aattcaaaga
      901 tttacaaatg ctgtttataa aggtcaatta tgggttcaac aagcatctag caaagaagta
      961 gctaaggcta tgcaaccttt ctttgaagat atatctttag aagatttaac aacagttgta
     1021 gatagatata aatcaataga tgcatggtct caaaaccctg tattaaaaga agaaggtctg
     1081 aagaaattaa tgactgttat gaaggaagct aaagaattag ataaggaagc accttataaa
     1141 gatatcgtaa acactgagtt tgcaaacaat gctataaaga atattaaata aaatatatta
     1201 cttttgccta gatattttct cgattttatg attaaaatta acaaaaatat aaaaagagcc
     1261 ttttaaaaga ctctttttat atttgagatt aaagtttata taataaaata gaatcaaagt
     1321 actaattgta cttatatatc tatcctctat attctgctcc agcattaatt tttgtaattt
     1381 gaacagctac catttcttca ttgcttccat tagtaatttc atgccattct cctgttggta
     1441 caaatacagt atcgccgttt tttacatcac attcatgctc accaagtttc attttacctt
     1501 ctccttttag gaacacaaat atttgttctc cattaggatg tctatgagca ttaagaactt
     1561 gattaggttt aaaataataa acttctgaag ttatatcgtt accagcaaat actggtattt
     1621 ttggaactgc tacatcatta tattgagcta agcttccatc taatagattc tttttatttt
     1681 catgcatact agaaatacct cttttctata ttatatattt actattatta ttccaatata
     1741 agaaaatagt attctcatct tgcataatag gaataaaa
//
LOCUS       DAMJVM010000196         1772 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.201, whole genome shotgun sequence.
ACCESSION   DAMJVM010000196 DAMJVM010000000
VERSION     DAMJVM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.201"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            112..1548
                     /gene="miaB"
                     /locus_tag="PC523_003480"
     CDS             112..1548
                     /gene="miaB"
                     /locus_tag="PC523_003480"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDF2476956.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPNMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESMNSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERIRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPE
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHERFNRVLEAVNRISAEINDGYKNRIVEVLVEGRSKNNENKLSGRTRQNKLVNFEGG
                     NDDLIGKLVMVEITEPRTFSLNGILVND"
BASE COUNT          750 a          170 c          310 g          542 t
ORIGIN      
        1 aaatttacta ttaaaacaca ctagaaaaat aataatatct ttaaaagatt tttcttattt
       61 gatataatta aggttatgtg taatataaga aattaactag gaggaataga agtgagcaaa
      121 agaaaacaag taacaatacc tatagagaaa atacaagaac aagataaata tatagaacaa
      181 atacacagac aaaatgagga atacttcaac agaactggaa agagaaaact cgtgtttact
      241 caaacctttg gatgccaaat gaatgaacat gattcagaaa aattatgttc aatgttagaa
      301 gagatgggat atcaaatgtc aatgatggtc gaagaaagtg atttaataat atataacaca
      361 tgtgcagtta gagaaaatgc agagttaaaa gtgtatggta atttaggaca attaaaacac
      421 ctaaaaggaa aaaatccaaa tatgaaaata gctgtatgtg gatgtatgat gcaacaacca
      481 catgttgtag aggaattaag aaaaaaatat aaacatgtag accttatatt tggaacacat
      541 aatctatata aatttccaca attgcttaca gaatcaatga attctgacaa aatgttagta
      601 gatgtatggg atgtggatgg agaggtaata gaaggtctta gaagtaatag aaaatttgaa
      661 ttaaaagcat ttgtaaatat aatgtatgga tgcaataatt tctgcacgta ttgtatagta
      721 ccatacacaa gaggaagaga aagaagtaga actcctgaag atataattaa cgagataaaa
      781 gagcttgttg caaatggaac gaaagaaata actttacttg gacaaaatgt agattcttat
      841 ggtaagacac ttgaaaatcc agtttcattc tcagaacttt taagaaaagt gaatgatata
      901 gaaggaattg aaaggatacg atttatgaca tctcatccaa aagatatatc tgatgaagta
      961 atatatgcaa ttagagattg tgataaggtg tgtgagtttt tacatttacc aatacaatgt
     1021 ggaagcagta gtcttttgaa aaaaatgaat agacattata caaaggaata ttatttagaa
     1081 ataatagaaa aagctaaaaa agaagttcca gagattgctt tttctacaga tttaatgatt
     1141 ggtttcccag gagaaacaga agaagattta ttggatactt tagatgtagt tgaaaaagtt
     1201 agatatgatt cagcttttac atttatttat tctaaaagac agggaactcc tgctgctaaa
     1261 atggaaaatc aaataccaga agatataaag catgagagat ttaatagagt attagaagct
     1321 gtaaatagga tttctgcaga aataaatgat ggatataaaa atagaatagt tgaagttcta
     1381 gttgaaggaa gaagtaaaaa taatgaaaat aagctttctg gtagaactag acaaaataaa
     1441 cttgtaaatt tcgaaggtgg aaatgatgat ttaattggta agttagttat ggttgaaatt
     1501 actgaaccta gaactttttc attaaatggg atactagtaa atgattaata ttgtataaaa
     1561 taataaaatt atgtaaacta atcgaaatag atgtactata gaacaaatta tattaaaaat
     1621 ttttatttta agcgagtttg tcatatgtta tgctaattta agtttttgct attaataaaa
     1681 ataaacttga tataagatat aatttcattg tacatctatt tttgttttat tatattttat
     1741 caaagcctac ttaattgtca ttagtagttt tc
//
LOCUS       DAMJVM010000197         1702 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.202, whole genome shotgun sequence.
ACCESSION   DAMJVM010000197 DAMJVM010000000
VERSION     DAMJVM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.202"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(142..1533)
                     /locus_tag="PC523_003481"
     CDS             complement(142..1533)
                     /locus_tag="PC523_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2476957.1"
                     /translation="MSSTESVSKKGGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSITMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEE"
BASE COUNT          612 a          315 c          182 g          593 t
ORIGIN      
        1 cacaattata attttaatta taatatctat ttgtgtaaag ctttttttca taatcaaaga
       61 ggaaattaca tttaatttta tatttaaatg taatttcctt tacttaaagt aattcatcta
      121 tttacaataa ttcgtcatga attattcttc ttttgttagt aaatccatag ccattagtct
      181 cgttaataca tttcttattc ccaataatat taaaccaaat attattaaaa ctgttgctat
      241 tcctccaaat atctgtaaaa aaccaagtgt agctgtaaaa ccagcaatct ttcctgataa
      301 taaacttgca aaaaaacttg ttagatacca tgctcccata gataacgtag catacttagc
      361 tggagctaat ttattgaaca ttgcaagtcc tataggagat aagaataatt cacctactgt
      421 tgtaaatata taagtcaata ctataaacaa tatactagcc tttaccattc catctggacc
      481 attagctaat gtagcaaata ccataactat aaacccaaca cccattatta tcattcctaa
      541 tcccatttta actggaatag agaaatcacc tcttttggta tgagataatt taacccatag
      601 ggtagctaat actggacata aagccacaca taatatagca tttagtgatg taagccatgc
      661 tactggcatt tcaaatccaa atagagttct atttacattg tctcttacat aaatcataaa
      721 agaactttgt gtttgatagt atgctgtcca gaataagacc acaaataaaa ataaaataac
      781 cattgctatt attctatttt tctccaactt agtaagtggt ccatactcaa ttttttcacc
      841 tttattgcct ttagtcacag gatattttcc cacttcattt aataattttg gtgcaagaag
      901 tgtaaatata atcataccta acatcatgcc tataccacaa attaaaaacg ccatcttata
      961 tccataagat actatatctc cacttgctcc tcttactgca aacacattat cagaaagtaa
     1021 tccacatatt attggagcta taaatgctcc tacatttact gccatataga ataaactgta
     1081 tgctgcatct tttcttgaca catcagtttt aggatatata tccccaacta tatttgaaca
     1141 acttgattta aaaaaaccat ttcctaaaat aattattgat agacctaccc aaaccatggt
     1201 aatagatgta gatgaagcaa atagtacgaa atctcctatt gctattaaaa gtgcacctat
     1261 agaaatacat ttttgttgac ctaaaacttt atctgctagc caaccaccga ctaatggtgt
     1321 aagatatata agacctgtaa ataaaccaaa catagatgtt gcttcttgag tagaaagacc
     1381 taatccacct tgagctacac ttgctgtaaa aaataatacc aataaagctg ttaaaccata
     1441 ataactaaat ctttcccata aaattgtcat gcaaataagc caaaaactct taggatgacc
     1501 acctttttta gatacgcttt ctgtacttga cataaaaaaa cgcccccttt tgttttatag
     1561 ttaaatttaa tattaagatt gctttaacca ttttagcaca tacttataat atcatctttt
     1621 ctatctttac tcaatagatt atcgcctgta ttttcatttt tttattatta tttttaaaat
     1681 tatgaaaaat attatttttt ca
//
LOCUS       DAMJVM010000198         1653 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.203, whole genome shotgun sequence.
ACCESSION   DAMJVM010000198 DAMJVM010000000
VERSION     DAMJVM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.203"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            107..1543
                     /locus_tag="PC523_003482"
     CDS             107..1543
                     /locus_tag="PC523_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HDF2476958.1"
                     /translation="MKFYKRILTLILSISFVFANIQLANAISSVEKIQGKNKYEIAGK
                     IADKTAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKSPIILTNGQSIPFSTSGLKSYAIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHEPMVLVNDGSNKSV
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGIVKNIKPTTRTIKDGMYKVGKDIS
                     AGEYLITSNSGSYDSYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMI
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNNSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
BASE COUNT          677 a          168 c          271 g          537 t
ORIGIN      
        1 atggtctaat ataatttgta attttacata agtttgttaa gtacataact aacctaagag
       61 tatgtattta tatattaaaa ttttattctg gggggacaat tttattatga aattctataa
      121 aagaatatta acattgattt taagtatttc ttttgtattt gcaaatattc aactagcaaa
      181 tgcaatatca tctgtagaaa aaatacaagg aaaaaataag tatgaaatcg caggtaagat
      241 agcagacaaa actgcatata aaactgctat attaattaat actagtaact ctatcgcaga
      301 tgggttaagt gcaagtggac tagcaggagc tttaaatgcc cctatactat taactgaaaa
      361 aaatactata ccaactgaaa cgtctgcaag attaaaaaat gtatcaaaag tttatataat
      421 tggaggaaca tattctatta gtacatctgt cgaaaattca ttaaaatcta agaaaatgaa
      481 agttgtaaga attaagggaa acgatagaat aaaaacaagt tataatgttg caaaagaaat
      541 aaattcgata aaaaaagtta atactgttat gttaaccaat gcatataaag gagaagcaga
      601 tgctataagt attgcatctg tagcagcaag agataaatca ccaataattc ttactaatgg
      661 acaaagtata cctttttcaa ctagtggttt aaagtcttat gcgataggag gaacagcttc
      721 aatgagtact actttagtaa acaatactaa atctaccaga ctaggaggga gtactcgttt
      781 tgaaactaat aaagcaataa taaataagtt ttataaagat gcgagagagt tttatatagc
      841 tggagcttat gaattaacaa atgcacttgt aggttcttct ttatcaaaac atgagccaat
      901 ggttttagtt aacgatggta gcaataaaag tgttttaaaa aatgctaaga aaataacttc
      961 cataggatat atagattcaa atatagtaca acaatgttta aatattacaa atggaattgg
     1021 agatattaat actggaattg taaaaaatat taagcctact acaaggacta taaaagatgg
     1081 aatgtataag gttggaaaag atatttcagc aggtgaatac ttaataactt caaattcagg
     1141 aagttatgat tcttattatg aagttacaag tgatagtaca ggtaatgctg atagtatact
     1201 ttcaaatgat atttttagtg ggactagata tatcacttta aagaatggtc aatatattaa
     1261 aattgaggat tcaacaatga tattagcaaa atatgctaaa gcccagaaag ccaagaatgg
     1321 taagtttgga aatggtatgt ataaaattgg attagaaatt cctgctggag aatatattat
     1381 aatgtcaaat tcttctgatg catattatga agttagaaat aatagcttag gtaatgcaga
     1441 aggaatagtt actaatgaca ctttttcagg aagaagatat ataacagttg aagaaggtca
     1501 atatttaata ttaaatgatt gttatttaat tgaaaatgaa tagatgtaat tgtatttaat
     1561 agtaagtaat atattttata taagaaagga tgttttaagt tttaatttaa aacatctttt
     1621 tttattatta taataaatta aatgattttt ata
//
LOCUS       DAMJVM010000199         1592 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.204, whole genome shotgun sequence.
ACCESSION   DAMJVM010000199 DAMJVM010000000
VERSION     DAMJVM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.204"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            158..715
                     /locus_tag="PC523_003483"
     CDS             158..715
                     /locus_tag="PC523_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2476959.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAENLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNNQMTKAVIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLM
                     SKEEAIKGLTDSIDTVLLGIHKKYD"
     gene            complement(1096..1569)
                     /locus_tag="PC523_003484"
     CDS             complement(1096..1569)
                     /locus_tag="PC523_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2476960.1"
                     /translation="MFNEHLIRELKEDDIKDALELIWNVFEEFESPDYCDEGIMTFHQ
                     FIEYNSIIEKVKEKTLFFWGCFDCEKLIGVLAIRDLNHICMLFVDKNYHKRGVAKKLM
                     TIGIEYCICNKFSLTEITVNSSPYAVEVYHKLGFKDTAVEQISDGIRFTPMKLLI"
BASE COUNT          634 a          192 c          207 g          559 t
ORIGIN      
        1 tattaaagct aatttaatta gtattatatt aaattataat taaactgtca agattttata
       61 atagtataaa gaattttata aattacaata attgacgaaa ctaattagat tagttaaact
      121 aataaagtaa ttaattttaa atgaaagagg gaaaatcatg cgtgttagta gagaaatagt
      181 tatacaagct acatcagata tagttaataa agatgggctt aacaaggttt ctttaaaagt
      241 tattgcagaa aatctaggaa tacgtacacc atctttgtac aatcatatag aaagtctaga
      301 tgatttatta cttgaagtag ctcataaagg aatgagagag atgaataatc aaatgacaaa
      361 ggcagttatt ggaaattttg gagatacagc tataaagtgt ataagtatag catattttaa
      421 ttttataatt tctaatcctg gtgtttatga aacaattcag tgggctactt ggcatggaaa
      481 taatgaaaca ttagaaatct ttgatgaata taaatcctta ttagaaaaac ttattctttc
      541 ttgcaatcta aatacaaaga atacaggtga agtattaaac ttaataatga gtgtacttca
      601 tggttattcg actttgcaac ttgggaaatc acttatgagt aaagaagaag ctataaaagg
      661 tttaactgat tctattgata ctgtattatt aggtattcat aaaaaatacg attaagtagg
      721 taaatcaact attttaacac atttattgaa caaattttca tgaggcatat aaattataat
      781 tccatgataa aaaattttac catttgtaac aaaggaaaag ctacaggata gaagtatatt
      841 gcaataatgt ttatttttta atggacaatc attcttacaa ataggagata tatatggaaa
      901 caacataagt taagcttaat aattgaattg atttatatat tagtcaaaaa aatatcaaaa
      961 aataaatcag agttccatat tagtaaggct tttaaggtat gttataaaaa aaataaaaaa
     1021 gatagtgcta tttttaaaat ttaatttaaa agcaaagtca ccataaagca ttcaagagac
     1081 tttgcttttt atttactata ttaacaattt cataggtgta aaacgtatac catcagaaat
     1141 ttgctcaact gcagtatctt taaaccctaa tttgtgatag acctcaacag cataaggtga
     1201 tgaatttaca gttatttcag ttaaactaaa tttattacaa atacagtatt ctatgcctat
     1261 agtcattaat ttttttgcaa caccacgttt atgatagttt ttatctacaa ataacataca
     1321 tatatgattt aaatctctaa tagctaagac accaatcagt ttttcacagt cgaaacaacc
     1381 ccaaaagaat agtgtttttt ctttcacttt ttcaataata gaattatatt ctataaattg
     1441 atgaaatgtc atgattcctt catcacaata atctggagat tcaaattctt caaatacatt
     1501 ccagattaat tctaatgcat ctttaatatc atcttctttt aattctctta ttaagtgttc
     1561 attgaacatt ataaacaaca cctctattat ta
//
LOCUS       DAMJVM010000200         1451 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.205, whole genome shotgun sequence.
ACCESSION   DAMJVM010000200 DAMJVM010000000
VERSION     DAMJVM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.205"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            48..659
                     /locus_tag="PC523_003485"
     CDS             48..659
                     /locus_tag="PC523_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDF2476961.1"
                     /translation="MINDIKNKFKDYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            964..1302
                     /locus_tag="PC523_003486"
     CDS             964..1302
                     /locus_tag="PC523_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HDF2476962.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVTFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
BASE COUNT          569 a          158 c          191 g          533 t
ORIGIN      
        1 aaaactcaat tatataattt ttaataatat tattttagga ggtaaaaatg ataaacgata
       61 ttaaaaataa attcaaagat tacaaaccat atataaatgg atacaagaat atgaagagag
      121 catctgtttt aattccaata gtagaaataa ataacacaca ctatatatta tttgaagttc
      181 gctcaaaaaa tttgaagcat caacctagcg aaatttcttt tccaggtggt aaaatagaat
      241 caggtgagac tccatatgaa gctgttatta gagaaacttg tgaggaactt ggaactttta
      301 gtgacaatat agaagtaata tcaagtcttg acctccttat aacaccagta aagtttatca
      361 tacatccata cttaggatat ataaaaaata ttaataattt aaatataaac aaggatgaag
      421 ttgaccatac ttttttggtt cctataaaat atcttttaga gaatcctcca aatacttata
      481 gtaatactgt aaaagttttt ccagatgaaa atctaccata tggttcaatt ccaaaccaaa
      541 aagattataa atttgaaagt ggaagttatc ctattctgtt ttataaatat aaagattatg
      601 taatttgggg aatgacagca aaaatactag aaaacttttt aaatttttta tcagaataac
      661 acttataata aatcattaaa acttttacat aagaaattca taaaagtttc taaatttaaa
      721 atttaacagt tctaaaatat caagaactat attttgtagc acttttttat tttattattg
      781 tttgaatatt tgatagtttt caatgcaatt tcaacataaa tactatgtat tagtgtatac
      841 tgttgctgaa ttaaagtatg ataatctatt taataaagta tgcagtaaat gattttaatg
      901 tgtctttatg gtaaaattac taataagtaa taaaattaat taaaatatat ggaggtgctt
      961 gtaatgtttg ctgtaggatt tgttgttata attgcacttg cctttttcat attaagtgta
     1021 ctattattta atggaaaagc cagtatgcta ctagcaggtt ataactctat gagtaagaaa
     1081 gaaaaagcac gatacgataa aaaaaaatta tgtagaagtt ctagcattgt tacttttatt
     1141 gtctctataa tgctatttat tatggcattt ttgggatata gaatagagag tggtcaaatg
     1201 gacgaaaaaa aaatggttac cttttctata atatttataa tagtcatact ttccagtgta
     1261 ttttttaata tcctctactc taacaaaaaa tgtaaaaaat aaatagttgt aagttgagca
     1321 gttaattata aaagattgac tgctttttcc tgtttattga ctactcttat taaatatttt
     1381 aacatttgaa aatctatatg aaaataatgt atatagtata aacttctaat aataatttta
     1441 tctttgtgtt t
//
LOCUS       DAMJVM010000201         1443 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.206, whole genome shotgun sequence.
ACCESSION   DAMJVM010000201 DAMJVM010000000
VERSION     DAMJVM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.206"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(97..648)
                     /locus_tag="PC523_003487"
     CDS             complement(97..648)
                     /locus_tag="PC523_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HDF2476963.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNREDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMHISYKMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
     misc_binding    complement(800..1063)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          484 a          231 c          147 g          581 t
ORIGIN      
        1 ctttgaacaa cataagaata aaatatttat aatctacttt attcttacgt tgttcagtta
       61 ttataatagt ttataaagta aaatattatt tcgtatttaa gcttgataac taactcttct
      121 tattgataga ctaacaatct tgaataaaaa tattcctgca actagattta ttaaaacagc
      181 tggtaatact actgctaaga ataaagctgt gaatgaagcc cctggaagac caactatagc
      241 ttgagctgct aataaaaaga tagttccact tacaagagtt cctattggca ttattattgc
      301 aactactaca gtatcttgtg ttttcttagc taattttttt atgaaaggca tcttataact
      361 aatgtgcatt aataaaaata ctatatttgc tgtaagagtt ttatctatta cattaggaat
      421 ttgacctccc ggaaaacttg atgtaagtgc tgtaaatatt cctgttacta tacctgctat
      481 taaacaagtt ttataatcct ctctatttaa aaccaatatt acaaataaca ttgctagtgg
      541 tacatctggt ttcattggta atcctatagc tggtgtgatt tgatgtatca ataatcccat
      601 cgctaaaagt attgcattta aagttaattt ttttgtattt gttttcattt tttgtacttc
      661 ctcctatttt tttaattttc aatttatttt tttgccaacc tactatatca tcatctttag
      721 ctttggaaat tcataacatc tttttcacca ccttctatct tttaaatttt cagtaaaata
      781 aaaaaactcc gtcctaaact aataggacgg agattatccg cgttgccacc taaattatat
      841 aaaaattaat ataaaattca attttcatat atctcattat caggtactta cataccttat
      901 tcgctataac gtgcgactga cgataaagtc tactctcaaa actatgattt caatttattc
      961 ctctaagggc cattcatcaa attttttctc gctaagattc caccatcctc agctctctat
     1021 actgaaccta aatttaacta ctcttccttg tcataggatt taatttattg tattaaattt
     1081 tgttgttaaa ataatgatat tcttttttct aaaaaatgtc aacacttttt ataaaaaata
     1141 tattttgtaa tctatttttt tataaaaaaa gtaaattaca ctatacattt ctttccacat
     1201 acaaaattct atgaataatt actttaatag tctaataatc tgatttttag ctctaattct
     1261 gcctttatta attttatgaa taattattga tgtaatttta tattttgatg catgaggaac
     1321 taaattcttc aaagttaatt aagtacattt atcaaaaatt catctcattt accatcattt
     1381 aatactcatt tctgtattat ttatatacct actttgaaac agaatactac tctgttttaa
     1441 tta
//
LOCUS       DAMJVM010000202         1417 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.207, whole genome shotgun sequence.
ACCESSION   DAMJVM010000202 DAMJVM010000000
VERSION     DAMJVM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.207"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(373..1254)
                     /locus_tag="PC523_003488"
     CDS             complement(373..1254)
                     /locus_tag="PC523_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HDF2476964.1"
                     /translation="MKITEGYMPFEGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIKELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSARLWEKEQKRRISYMSESDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGARDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIEVLTEWLDAND"
BASE COUNT          465 a          277 c          156 g          519 t
ORIGIN      
        1 atatatattg tatataaaaa aggttgttgc tctacagata cttctatcct ccattataag
       61 gatactccat tatcatgtaa atgaactgga taactaccac aatggatagt gataacttcc
      121 tgaacaatat ttatttttat tatcatgatt tcctcatgat gaatcattta ttcttgaatg
      181 aacatataaa aagctcacta tttaattaca attatactca ttactacaac tattaatttt
      241 ttcttagaca caatagattt taaaataagt acaaatatac catcgtttat aatataaact
      301 attacacttt tatcatacca aaaagactag acaacatgtc tagcctctct tttgtataaa
      361 tttatattat ttttaatcgt tagcatctaa ccactcagtt aagacttcta tatatttctc
      421 attttcttca acgaatggca tatgtcttga atactcgaat aactcccact tactattagg
      481 tattctatca tacatagtct ttgcaatata tggtgaacat aaatctcttg ctccactagt
      541 tactagacaa ggctctttta tttcatgtaa tctatctgta aactcatatc cagaaagagt
      601 tcctgtagga gaaaattcat tttgtcccca tcctacaata tatgcttcag aacctgattt
      661 tttaggtctt cttaggcact ctggagaatc ttctgtcact tcacctgcac aatacatttc
      721 cataaatctt tctaatgcat cattgtattc tttactagag taatctccag tatttactgc
      781 atcaagtaat gctttttggt cagactcact catatatgat attcttcttt tttgttcttt
      841 ctcccaaagt cttgcagaag aaagagtaga tgaaaggatg taagacttaa taccttttgg
      901 tttatattca atagcatacc aaatagcctg catccctccc caagattgac ctaacagatg
      961 aatttcatca agccctagat gttttctcag ttcaataagc tcttttatcc aagtatctgc
     1021 attaaataac tcaggatgcc cttctacaaa tgaattacca caaccaattt ggtcatacat
     1081 tataacttgt ctaccacttt cagctatctt gtctaacact tcaaaatagt tgtgtgtaga
     1141 acctggacct ccgtgaagta gtactaatgg cttcttccct tccgtatttt ctcctactat
     1201 acgataataa gttttaaaac cttcaaaagg catatatcct tcagtaattt tcatgtttat
     1261 ctcctccaat ttgctattat gaacaataat caagttcatt aataaggcta cttaaaactt
     1321 ctcatacata tttaataatg tattttaatg tttctatttg ctttcataaa tagtataaca
     1381 caaaagaaaa aaatctccat accaaatcaa ttataaa
//
LOCUS       DAMJVM010000203         1215 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.208, whole genome shotgun sequence.
ACCESSION   DAMJVM010000203 DAMJVM010000000
VERSION     DAMJVM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.208"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            122..1198
                     /locus_tag="PC523_003489"
     CDS             122..1198
                     /locus_tag="PC523_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2476965.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASVLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYKDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
BASE COUNT          476 a          174 c          213 g          352 t
ORIGIN      
        1 aattatatat aaaaaataaa ttttatgttg acatagttaa ttcagcttga tattatagtt
       61 attaaaaata aggacagtaa aaacgctatc catattacaa actaaaaagg ggggaattat
      121 aatgagtaag aaattattta ttcctggtcc tattgatgtt aaagaagaag tcttgcaaaa
      181 gatggctaca ccaatgatag gacatagagg aaaagatgca tctgtactcc aaaagagtat
      241 aagtgaaaaa atgcaaaaac tattctatac gaacaacacg atacttttat ccacgtcatc
      301 cggaactggt ttaatggaag gctccattcg ttcctgtaca tccaaaaagg cagcagtatt
      361 ttcctgtgga tcctttggtg atagatggta caaaatggca gtggctaata atgtcccagc
      421 ggatatattt aaagttgaat taggagaggc aacaacgcca gaaatggttg ataaggtgct
      481 ttctaccgga gaatacgatt taataactgt aactcacaat gaaacttcaa ctggaataag
      541 aaatccaata gaagaaattg gagaagttgt taaaaagtat aaagatgtaa tctactgtgt
      601 agatactgta agttcagccg gtggaataaa agtcgaggtt gataagattg gaatagatat
      661 ttgtataaca tcagtacaaa aagcactggg attaccacca ggaatgtcta tttgcacatt
      721 ttctcaaaaa gcaatcgata gagctaagca agtacctttt agaggagtat atcttgattt
      781 gttagcaatg tatgaatact taatcaagaa aaactaccaa tacccttcaa caccatctct
      841 ttctcacatg ttcgctttag atttccaatt agataatata ttagatgaag gcctagataa
      901 cagattcaac agacatgaag acatggcaaa cttagttaga aactgggcta aaaaacactt
      961 ccaaattttt accaatgaaa atcacttatc caatacactt actgttatag aaaatactca
     1021 aggtattagt gtatcaaatt taaattcaaa attacaagaa cgaggattcc aaatagcaaa
     1081 tggctatgga gatttaaaag aaaaaacttt tagaatttca cacatgggtg actatactgt
     1141 cgaagatgta caagaattat tagataatat tgatgatata ttaggtttta ataaataata
     1201 ttgtaaaaat atgta
//
LOCUS       DAMJVM010000204          912 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.209, whole genome shotgun sequence.
ACCESSION   DAMJVM010000204 DAMJVM010000000
VERSION     DAMJVM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.209"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            154..660
                     /locus_tag="PC523_003490"
     CDS             154..660
                     /locus_tag="PC523_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HDF2476966.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
BASE COUNT          367 a           84 c          147 g          314 t
ORIGIN      
        1 taaaaactac ttattgcgaa agtaattgat ttgttgtatt ataatttaca tgaattatgt
       61 aagacaatca aaattcagta ttataagagt acatatattc aaatttttaa caattaattt
      121 atataattaa aaaatgaagg gtaggatgaa gtcatgggtt ttagagaagt aaaaattgag
      181 gaactacagt ttaatccatt tactaagatt ggcaaagaat ggttacttat aacagctggt
      241 gattcagaaa agtttaacac aatgactgct agctggggtg gtgttggagt atattggggt
      301 aaaaatgttg ttacaactta cataagacca cagagatata caaaagaatt tgtagatagt
      361 aatgatacat ttacagttgc cttctttgat gaaacgtata gagaagcttt aaatatttgt
      421 ggtactattt ctggtagaga tataaataaa atagaaaaag caggacttac accatatttt
      481 gtggatgaca cagtagcttt tgaagaagct aatatgatta tagtatgtaa aaagttatat
      541 catggtaata tgccaccaga aaactttgat gctaaagaaa atgataaaaa atggtatcca
      601 gagaaagact atcatactat gtacatttca gaaattataa aagttcttgt aaaagaataa
      661 ttataataaa aagtatctaa aaagaactta ttataagtaa atgattgtac tttcttatct
      721 tatatttaag tagatgctaa taagtaaata aatattaatt ttaaataaat actattataa
      781 aaaagatgaa ctatatttgc tagagttcat ctttttattt atgtaagcac ttatataagt
      841 agagatttac ttgagtaaaa gtatttattt gagatatttg agtacacttt ataagtattt
      901 ttatgctaac ta
//
LOCUS       DAMJVM010000205          903 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.210, whole genome shotgun sequence.
ACCESSION   DAMJVM010000205 DAMJVM010000000
VERSION     DAMJVM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.210"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            139..>903
                     /locus_tag="PC523_003491"
     CDS             139..>903
                     /locus_tag="PC523_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HDF2476967.1"
                     /translation="MNCTNKKILILSLSMITILASVGEVNALEVKNNSNLEKQTIKET
                     VVPELVGLKMQEAFPDENFRKYIFGILVTLDISDIRDDTTINSEIKQKIEGIKDLSLE
                     YESIKDLTGINYFTGLEKLYCSSNELKKLDLSKNTKLEELNASSNKLKTINLNGSEKL
                     KTLLCNDNELSNLDVSKNTKLKYLYCDNNRLEKLDITNNKNLVGYSWGNQKEPEKPSE
                     PEKPSGGGSYTPAPIVTKINSLVGSDRYATSIKISKQ"
BASE COUNT          400 a           87 c          132 g          284 t
ORIGIN      
        1 ttaaaatcat aaaaatattt cgattatttt aatataaata attaatcata gataagatat
       61 atttttgaat tattgctaaa tttatcaaga gatgataaaa tcaggaaatg tgtattattt
      121 ttataggagg aatattaagt gaattgtact aataaaaaaa tacttatttt atcattatct
      181 atgataacaa ttttggcttc agtaggagaa gtaaatgcac tagaagtcaa aaataatagc
      241 aatttagaaa aacaaacaat aaaggagact gttgtacctg agttagtagg attaaaaatg
      301 caagaagctt ttccagatga aaattttaga aaatacattt ttggtatttt agtaactttg
      361 gatatttcag atattagaga tgatactact ataaatagcg aaataaaaca gaaaattgaa
      421 ggaataaaag acttaagttt agagtatgaa tctataaaag atttaacagg aattaattat
      481 tttactggat tagaaaaatt atattgttct tctaatgaac taaagaaatt agatttaagt
      541 aaaaatacta aattagaaga gttaaacgct agttctaata aattaaaaac aataaattta
      601 aatggaagtg aaaaacttaa aactttattg tgtaacgata atgaactaag caatctagat
      661 gtaagtaaaa atacaaaact taaatattta tattgtgata acaatagact agaaaaatta
      721 gatattacta acaataaaaa tcttgtaggt tactcttggg gaaaccagaa agagccagaa
      781 aaaccatctg agccagagaa accgtctgga ggaggttctt atactccagc tccaatagtt
      841 acaaaaataa attctttagt aggttctgat agatatgcta cctcaataaa aataagtaaa
      901 caa
//
LOCUS       DAMJVM010000206          868 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.211, whole genome shotgun sequence.
ACCESSION   DAMJVM010000206 DAMJVM010000000
VERSION     DAMJVM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.211"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(356..808)
                     /locus_tag="PC523_003492"
                     /pseudo
     CDS             complement(356..808)
                     /locus_tag="PC523_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
BASE COUNT          330 a          128 c          102 g          308 t
ORIGIN      
        1 ggagttttat ggcacatatg ttttgatgat atttattatt tattaaaaga aacaatgagg
       61 ttaaaattaa tatcatataa taaaattgta ttactttaaa acgatttatc aataggtaaa
      121 atttatgagg tatgaataca ataacaatat agttttacga ttgtagatat caaaaaatgt
      181 atgttactag aaaggcgttt tgtagattta tatatgatgt aatcattaaa gctataaata
      241 aactaaataa aaagagtatg ttttaaccta cgcttttaga tttgaaatat actcttttat
      301 taattatagt tatacaaatt aaccagatta tatataatta aaatttttat aataattata
      361 tattaggttc ttcaattagt tttccacata catatctatg aatgatattt ctatcatcac
      421 caacatagat aaatctttca agtctttcaa gtatagaata attgtcatga tttaaatctg
      481 aatcatcaat aatcaatgca tcaaaatcat aaccttcctc aaagctacca acttttccaa
      541 agaaacttcc accacttttg gttgcaatat aaaaagcttc agataaagac aagaaattgt
      601 acttcttacc agagttaacc caatataatt tagataattg tattgcagat accattgctt
      661 taaatattga caatgtatgc ccaccactta tatctgaacc aagtgctaat ctaatattat
      721 tatcgattaa ttttctgata ggcatagcac cactaccaac attaaagtta gaagctggac
      781 agtgaactgc cataacattg ttttctctcc tgaacctccc atgactaaag tcacagggtt
      841 cctgcttcat agaattttgc atactaaa
//
LOCUS       DAMJVM010000207          851 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.212, whole genome shotgun sequence.
ACCESSION   DAMJVM010000207 DAMJVM010000000
VERSION     DAMJVM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.212"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(158..>851)
                     /locus_tag="PC523_003493"
     CDS             complement(158..>851)
                     /locus_tag="PC523_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2476968.1"
                     /translation="GYSDVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYD
                     LSTVELISHPNCFIPKKHGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIP
                     SINNFEKRAKIGKCKIGDLWEDDYVLDDSAIICKTGKGIFIVTGCSHSGICNIVEYAK
                     KVCGDDRVIGILGGFHLFELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILN
                     VNEVGVGLTISI"
BASE COUNT          311 a          156 c           69 g          315 t
ORIGIN      
        1 ttattgatga aaatatattc ttattaaaaa taaatttttt cacaatattt catataaaag
       61 tacattgatt tctcttaaaa taaacataat aaaaaagata ctttctatag aaaatatctt
      121 tttaaccttc aacttaaatc ttatatactt tcaaatacta tatgcttata gtcaatccta
      181 cccctacttc atttacattc aatatttcat tcatcttagc ttttacctta aaagaaacac
      241 aatgacaagg gtacaacatt cttatttctt ctttatccaa atattgtatt gtgctatcca
      301 gcctattatt caattcaaaa agatgaaaac cacccagtat accaataact ctatcatctc
      361 cacatacctt tttagcatac tctactatat tacaaattcc actatgagaa catcctgtaa
      421 caataaaaat acctttacca gttttacaaa ttatagcaga gtcatctaaa acataatcat
      481 cttcccacaa atctcctatt ttacatttac caattttagc tcttttttca aaattattta
      541 tagaaggtat ttcacctaaa aacacacaat tcttacttag attaaatggc ttatccttag
      601 gattatacat aaaaatattt ttaatttcct cagcagaaaa tggagctcct atgcttttct
      661 ctccatgttt tttaggtata aaacagttag gatgtgaaat aagttcaact gtagacaaat
      721 catatctatt ttctaaaaac tgtatacctc ttgtatggtc attatgacca tgagaaaaaa
      781 caacatgtgt taaatttcct aaatctatat ttaatttttc agcgtttgat atgaatacat
      841 ctgaatatcc c
//
LOCUS       DAMJVM010000208          832 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.213, whole genome shotgun sequence.
ACCESSION   DAMJVM010000208 DAMJVM010000000
VERSION     DAMJVM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.213"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            14..104
                     /locus_tag="PC523_003494"
     tRNA            14..104
                     /locus_tag="PC523_003494"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48..50,aa:Ser,seq:gct)
     gene            113..189
                     /locus_tag="PC523_003495"
     tRNA            113..189
                     /locus_tag="PC523_003495"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:147..149,aa:Pro,seq:tgg)
     gene            274..349
                     /locus_tag="PC523_003496"
     tRNA            274..349
                     /locus_tag="PC523_003496"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:307..309,aa:Trp,seq:cca)
     gene            410..486
                     /locus_tag="PC523_003497"
     tRNA            410..486
                     /locus_tag="PC523_003497"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:444..446,aa:Pro,seq:tgg)
     gene            493..569
                     /locus_tag="PC523_003498"
     tRNA            493..569
                     /locus_tag="PC523_003498"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:527..529,aa:Ile,seq:gat)
     gene            573..648
                     /locus_tag="PC523_003499"
     tRNA            573..648
                     /locus_tag="PC523_003499"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:606..608,aa:Phe,seq:gaa)
     gene            656..732
                     /locus_tag="PC523_003500"
     tRNA            656..732
                     /locus_tag="PC523_003500"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:690..692,aa:Met,seq:cat)
BASE COUNT          219 a          182 c          225 g          206 t
ORIGIN      
        1 aataactgaa tttggagaag tactcaagtg gctcaagagg atcccctgct aagggattag
       61 gctgggtaac cggtgcgagg gttcgaatcc ctccttctcc gccattcaaa aacgaggtgt
      121 agcgcagttt ggtagcgcac atggtttggg accatggggc cgggggttcg agtcccttca
      181 cctcgaccaa cttagctttt agttcggttg gttagagtgc ccaactcata accagagatt
      241 ctggggattg agtccatgaa ggttattctt cataggggtg tagctcagtt ggtagagcgt
      301 cggtctccaa aaccgtgcgc cgggggttcg agtccctcca cccctgccaa ataaaaagga
      361 cttgaaaaaa gttgggtttt agagtagtat aagatagtaa ataagatttc gaggtgtagc
      421 gcagtttggt agcgcacatg gtttgggacc atggggccgg gggttcgagt cccttcacct
      481 cgaccaagaa aagggcctat agctcaggtg gttagagcgc acgcctgata agcgtgaggt
      541 cgctggttcg agtccagcta ggcccaccat ttgcccagat agctcagtcg gtagagcagg
      601 ggactgaaaa tccccgtgtc ggtggttcga ttccgcctct gggcaccaga aatacggcgg
      661 tatagcttag ttggctagag cgttcggttc atacccgaaa ggtcataggt tcgactccta
      721 ttaccgctac catctaacta tatcaataaa gaactttgaa aattaaacag taggttaatt
      781 tataaacaag aaacaaacca taaagccaga tattttgata acaatagtat ct
//
LOCUS       DAMJVM010000209          815 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.214, whole genome shotgun sequence.
ACCESSION   DAMJVM010000209 DAMJVM010000000
VERSION     DAMJVM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.214"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            70..768
                     /locus_tag="PC523_003501"
     CDS             70..768
                     /locus_tag="PC523_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2476969.1"
                     /translation="MEKKYFKIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPQECQVLSVDLPEHGERKGEKNSFDPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPIIQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTIMEDGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT          302 a          106 c          134 g          273 t
ORIGIN      
        1 attacaacat tattttatag tttgattaat ttaacaatca ctaatatatc aaactgagag
       61 gagattttta tggaaaaaaa atatttcaaa attcatggag ttccttcaat tctttgggga
      121 aatccttcaa gtaaattata tatttacata catggacaat gtggcaataa agaggaggct
      181 gaagcttttg ctaatattgc agtcccacag gagtgtcaag ttttaagtgt tgatttacct
      241 gaacatggag agcgtaaagg agaaaaaaat tcatttgacc catggcatgt tgttccagaa
      301 ctagtatcta ttatggaata tgcaaagtgc cactggaatc aaatttcgtt atatgcaaat
      361 agcattggag catggtttag tatgttaagc tttgaaaaag agaacttaag agagtgtatt
      421 tttgtatctc caattctaga tatgcaacag cttatttcaa atatgatgct ttgggcaaat
      481 gtatcagaag aacagcttaa acatgaacta attatcccaa cttcatttgg gcatactctt
      541 tcatgggaat atctacaata tgtaaaacaa catcctatca tccaatggaa tgtaccaaca
      601 aaaatattat atggtaaaca tgatgagctt actgaaattg gtgttgtaga gaaatttgta
      661 gaaaagttta attgcaacct tactattatg gaagatggca aacattggtt taacacaccc
      721 caagaattaa attcattata tgaatggtta aatactttag ttaaataaaa aataattaaa
      781 ttatttataa atgttattta attaatttta tttta
//
LOCUS       DAMJVM010000210          813 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.215, whole genome shotgun sequence.
ACCESSION   DAMJVM010000210 DAMJVM010000000
VERSION     DAMJVM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.215"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            100..216
                     /gene="rrf"
                     /locus_tag="PC523_003502"
     rRNA            100..216
                     /gene="rrf"
                     /locus_tag="PC523_003502"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            223..297
                     /locus_tag="PC523_003503"
     tRNA            223..297
                     /locus_tag="PC523_003503"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:255..257,aa:Asn,seq:gtt)
     gene            302..376
                     /locus_tag="PC523_003504"
     tRNA            302..376
                     /locus_tag="PC523_003504"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:335..337,aa:Glu,seq:ttc)
     gene            382..457
                     /locus_tag="PC523_003505"
     tRNA            382..457
                     /locus_tag="PC523_003505"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:415..417,aa:Val,seq:tac)
     gene            463..539
                     /locus_tag="PC523_003506"
     tRNA            463..539
                     /locus_tag="PC523_003506"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:497..499,aa:Asp,seq:gtc)
     gene            551..625
                     /locus_tag="PC523_003507"
     tRNA            551..625
                     /locus_tag="PC523_003507"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:583..585,aa:Thr,seq:tgt)
     gene            639..723
                     /locus_tag="PC523_003508"
     tRNA            639..723
                     /locus_tag="PC523_003508"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:673..675,aa:Tyr,seq:gta)
     gene            733..806
                     /locus_tag="PC523_003509"
     tRNA            733..806
                     /locus_tag="PC523_003509"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:765..767,aa:Gly,seq:tcc)
BASE COUNT          220 a          186 c          208 g          199 t
ORIGIN      
        1 tgtagttttt agagtgttaa ctctaaaaaa ttaaaaaaag tattgacaaa aaaagtaaaa
       61 aatgttaata ttatacttgt cctagcaaca agacaaaaat gtggttataa tagcagagaa
      121 gatacacctg ttcccattcc gaacacagaa gttaagttct ctagcgctga tggtacttgg
      181 tgggaaactg cctgggagag taggacatag ccacgtaaat gttccaaggt agctcaacgg
      241 tggagcaacc ggctgttaac cggtaggctg tgggttcgag ccccaccctt ggagccatta
      301 aggctcgttg gtcaagaggt taagacaccg ccctttcacg gcggtaacag gggttcaatt
      361 cccctacgag tcaccatttg tgggatcata gctcagctgg gagagcacct gccttacaag
      421 cagggggtca caggttcgag ccctgttggt cccaccattt gcggcctggt agttcagttg
      481 gttagaatgc cagcctgtca cgctggaggt cgagggttcg aaccccttcc aggtcgccaa
      541 aataaaaaat gctggtgtgg ctcaacggta gagcagctga cttgtaatca gcaggttgta
      601 ggttcgattc ctatcaccag ctccatgtaa attaatacgg aggatttccc gagcggccaa
      661 agggggcaga ctgtaaatct gttgtcatcg acttcggtgg ttcgaatcca ccatcctcca
      721 ccaattaaat atgcgggtgt agctcaatgg tagagttctg gccttccaag ccagctgtga
      781 gggttcgatc cccttcaccc gctccaaaga aag
//
LOCUS       DAMJVM010000211          797 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.216, whole genome shotgun sequence.
ACCESSION   DAMJVM010000211 DAMJVM010000000
VERSION     DAMJVM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.216"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(108..593)
                     /locus_tag="PC523_003510"
     CDS             complement(108..593)
                     /locus_tag="PC523_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2476970.1"
                     /translation="MISELMLKNFGESSINFKTKIKLNLREKPDLNSLKLKSIPGGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNDSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKIITTIPDGFKVEVCYNVGKWARVNIDKNEKRYSGYVYNQYIEEYLG
                     L"
     regulatory      complement(676..769)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          262 a          129 c           60 g          346 t
ORIGIN      
        1 atttttttct ctttagttct ataataatgt ttcgacaaaa aaagaacccc tttaaaggga
       61 ttcttttttg taatttatta atatatttaa agctcactag tatcatatta taaacctaaa
      121 tattcttcta tgtattgatt atatacatat ccagaatatc gtttttcatt tttatcaata
      181 ttaactcttg cccatttccc aacgttataa catacttcaa ctttaaaacc atcaggaatt
      241 gtagttataa ttttagattc taatgttcta tcatttctaa gttctaaact taatccattt
      301 gttcttatat ttcctatata attctttttt ctatttttta ttgtatcatt actatcattt
      361 ttagcattac ttaatctttc taaatattta taaagtaacc agcctttgtc ataattactc
      421 tcaacttctg cccatattcc atctacacat tttaacttaa caattttacc ccctggtata
      481 ctttttaatt ttaaactatt taggtctggt ttttctctta aattcaattt aattttagtc
      541 ttaaaattga tgctagattc tccaaaattt tttaacatta attcagatat catcttaagc
      601 cctcctcttg ccaaatatta agttattaaa atatataact ctaaaaatat ttttaacaaa
      661 aagaatcatt atctttttgg caactggctg acatgacctt tattatataa agattttagt
      721 ccctatagtt ttgcgtcatt agatttatct aatattgcct atatatgaat gataacataa
      781 tttaaacaat taaaaaa
//
LOCUS       DAMJVM010000212          747 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.217, whole genome shotgun sequence.
ACCESSION   DAMJVM010000212 DAMJVM010000000
VERSION     DAMJVM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.217"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            99..>747
                     /locus_tag="PC523_003511"
     CDS             99..>747
                     /locus_tag="PC523_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2476971.1"
                     /translation="MLDTFITIVSSIIEIWSCKKVFDYTSQTKTDLKKLNMYFFFSVF
                     LIISFLYINIGANSRILICIFVMFIFYKSNYKVNIYKCLVISFFYWLILIGINGLSML
                     LVATVSNLDNMNVLLSGSLYRYEAIFLGKLILGILLYIYKISFKNFKISKKEIYICIP
                     MIANLLSFFVLYDYILNLVKISPERRTAFLVIVLLLILSNISIFVLIHIILKIKIR"
BASE COUNT          273 a           57 c           89 g          328 t
ORIGIN      
        1 tattaaattt aagtgtattt tttatgatat aattagtata tattttttat gatatatttt
       61 ataaaaaata taatttaatc atttttgagg atattattat gctagacaca tttataacaa
      121 ttgtttcttc tattatagaa atatggtcat gtaaaaaagt ttttgattat acgagtcaga
      181 caaagactga cttaaaaaaa ctaaatatgt atttcttctt ttctgttttt cttattattt
      241 cttttttata tataaatata ggagctaata gtagaatctt gatatgtata tttgtaatgt
      301 tcattttcta taagtctaac tataaagtta atatatataa atgtctagtg atatcatttt
      361 tttattggtt aatattaata ggtataaatg gattaagtat gttattagta gcaacagtaa
      421 gtaatttgga taatatgaat gttttactta gtggaagttt atatagatat gaagcaatat
      481 tcttgggaaa gttaatatta ggtatcttgc tgtatattta taaaatttct tttaaaaatt
      541 ttaaaataag taaaaaagag atatacatat gtattcctat gatagcaaat ctattgtcat
      601 tttttgtact ttatgattat attcttaatc tcgtaaaaat ttctcctgaa agaagaactg
      661 cttttttagt aatagtttta ctattaatat tatctaacat ttcaatcttt gtattaatac
      721 atataatact taagattaaa attagga
//
LOCUS       DAMJVM010000213          655 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.218, whole genome shotgun sequence.
ACCESSION   DAMJVM010000213 DAMJVM010000000
VERSION     DAMJVM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.218"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(<1..>655)
                     /locus_tag="PC523_003512"
     rRNA            complement(<1..>655)
                     /locus_tag="PC523_003512"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          143 a          205 c          149 g          158 t
ORIGIN      
        1 agttggcttc gcgcttagat gctttcagcg cttatccatt ccgtacatag ctacccagcc
       61 atgcccttgg cagaacaact ggtacaccag aggtacgtcc atcccggtcc tctcgtacta
      121 aggacaggtc ttctcaaatt tcctacgcct gcgacggata gggaccgaac tgtctcacga
      181 cgttctgaac ccagctcgcg taccacttta atgggcgaac agcccaaccc ttgggaccta
      241 ctacagcccc aggatgtgat gagccgacat cgaggtgcca aacctccccg tcgatgtgga
      301 ctcttggggg agataagcct gttatcccca gggtagcttt tatccgttga gcgatggccc
      361 ttccacgcag aaccaccgga tcactaagtc cgattttcat ccttgctcga cctgtatgtc
      421 ttgcaatcaa gctctctttt gcctttacac tctacgtacg atttccgacc gtactgagag
      481 aacctttgag cgcctccgtt accttttggg aggcgaccgc cccagtcaaa ctgcccacct
      541 gacagtgtcc caagaccaga ttcatggcct atggttagag tcccagtact acaagggtgg
      601 tatcccaagg atggctccgc caaaactggc gtcctggttt caaagcctcc cacct
//
LOCUS       DAMJVM010000214          648 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.219, whole genome shotgun sequence.
ACCESSION   DAMJVM010000214 DAMJVM010000000
VERSION     DAMJVM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.219"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(142..621)
                     /locus_tag="PC523_003513"
     CDS             complement(142..621)
                     /locus_tag="PC523_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDF2476972.1"
                     /translation="MSNKIIKNLLSGILQILFFLLGLVIVVGGFKSFIYLCFSGEATL
                     QGTISGILMFILGVSYFIIIKLLIEVLGSSEYSLFVKENVKRFRVIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVLVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT          256 a          106 c           45 g          241 t
ORIGIN      
        1 aaacataatt atatctttgc attataactt tttcatttta gcattatagt ttatgcatta
       61 tactttaata tattttattt taaattacta gttaacctct cttttcaagt tatctatctt
      121 ctatatatta aagtatagtt cctaaatagt taaatcatta tcctctttta tctttattgc
      181 ctttacaaat ccatctgcaa ttacaaaact cataagagaa gttataaaat atacaagtac
      241 tggaacagta aaataaaatc caaatcctaa atctaggaat ctcattcctt ttccagtagt
      301 accaaaagtt gaaataaact caattagaga gtttaaaagc aaaagatatc caataaccct
      361 aaatcttttt acattctctt ttacaaatag gctatattca gaactaccta atacttctat
      421 caataatttt ataataataa aatatgatac acctaaaata aacattaata ttccagaaat
      481 agtgccttgt agagttgctt ctccactaaa acataggtat ataaaacttt taaatcctcc
      541 cacaactatt actaatccta aaagaaaaaa taaaatttgt aatataccac ttaataaatt
      601 ttttataatt ttattagaca tcataccaac tcctttaaaa tattttat
//
LOCUS       DAMJVM010000215          587 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.220, whole genome shotgun sequence.
ACCESSION   DAMJVM010000215 DAMJVM010000000
VERSION     DAMJVM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.220"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            <1..>587
                     /locus_tag="PC523_003514"
     CDS             <1..>587
                     /locus_tag="PC523_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2476973.1"
                     /translation="LILSLSIITILASVGEVNALEVKNNSNLEKQTIKETVVPELVGL
                     KMQEAFPDENFRKCISDILNIKDSGDTVITDEMKKTIESTTILYITTESIKDLTGINY
                     FTGLKELHCSSNELKELDVSKNTKLRNLYCDNNRLEKLDITNNKNLVHYSWGNQKEPE
                     KPSGGGSYTPTPIVTKINSLVGSDRYATSIKISKQ"
BASE COUNT          253 a           71 c           89 g          174 t
ORIGIN      
        1 tacttatttt atcattatcg ataataacaa ttttggcttc agtaggagaa gtaaatgcac
       61 tagaagtcaa aaataatagc aatttagaaa aacaaacaat aaaggagact gttgtacctg
      121 agttagtagg attaaaaatg caagaagctt ttccagatga aaattttaga aaatgtattt
      181 ctgatatttt gaatattaaa gatagtggtg atactgttat aaccgacgaa atgaaaaaaa
      241 ctattgaaag cactacaata ttatacataa cgactgaatc tataaaagat ttaacaggaa
      301 ttaattattt tactggacta aaagaattac attgttcttc taatgaacta aaggaattag
      361 atgtaagtaa aaatacaaaa cttagaaatt tatattgtga taacaataga ctagaaaaat
      421 tagatattac taacaataaa aatcttgtac attactcttg gggaaaccag aaagagccag
      481 agaaaccgtc tggaggaggt tcttatactc caactccaat agttacaaaa ataaattctt
      541 tagtaggttc tgatagatat gctacctcaa taaaaataag taaacaa
//
LOCUS       DAMJVM010000216          577 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.221, whole genome shotgun sequence.
ACCESSION   DAMJVM010000216 DAMJVM010000000
VERSION     DAMJVM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.221"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            <1..>577
                     /locus_tag="PC523_003515"
     rRNA            <1..>577
                     /locus_tag="PC523_003515"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          154 a          121 c          190 g          112 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtac
//
LOCUS       DAMJVM010000217          577 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.222, whole genome shotgun sequence.
ACCESSION   DAMJVM010000217 DAMJVM010000000
VERSION     DAMJVM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.222"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
BASE COUNT          235 a           42 c           73 g          227 t
ORIGIN      
        1 cgctaaagat aagaatgtat aaggtggtaa atttgtataa aaaagaatat gagttgtaaa
       61 atattaaatg atattatact aattggaata attttaactt tattatatat tattttaata
      121 ccattagtac tgatgatgta ttttaagagt ggtataagca ttacaaatac gaatatgcca
      181 actgtaatat aaataatata agtagctata tacatatgtg ctgtcccata tattgtatca
      241 ttgtttatat taaaaagctt atgtaattgg tagcaatcaa taaataattt tcaaaagaaa
      301 tacctaaaaa cttaaaaaat acatatatgt acatttagtt agatttgaga tttttatata
      361 taattttgta gtgacaatat tatatattgt tattatgtct atttatatgc aattactata
      421 atatcagaag ttatagtatt atttgtttca ttatcaatag gattttttac tttagttttg
      481 tcaattttat ttaaaaaaat tattgatata aaagatgaga aggataaaac tacatgatag
      541 atttcaaaca taagagtgga tatttatata atagata
//
LOCUS       DAMJVM010000218          563 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.223, whole genome shotgun sequence.
ACCESSION   DAMJVM010000218 DAMJVM010000000
VERSION     DAMJVM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.223"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
BASE COUNT          204 a           55 c           65 g          239 t
ORIGIN      
        1 aaatatttct ttattttcat aatcttttat gacttattta tatttttcat ttttataaaa
       61 aaacaataat taagaatata actatattct acacataaaa atttttttgt ttaaacttaa
      121 ttttctgata atttgataat ttttcaaaat ttatagttga cttttatttt ttactgtatt
      181 aaactaatta ctatacaaaa cggaaaacaa aattttcaaa attattaaat taaaaggaga
      241 ggtaaatatg aagactttat taaacaaaca agtacgattt tttagtagcc aatttagtta
      301 gggtttgtac ctaagttaag acgaattgtc ataacataga tacaagcccc ttaggattat
      361 gatttttaag gattgtatct atgttggcta tagtaattgt tgttaaagtt tttattttat
      421 catcataata tagagccaca tggattatac tgtgtggctt attcgtattt ttgccataca
      481 gttgtatata ctgtgtggtt tttttatatc aaaattgagt tttcaaatta aattcaaaaa
      541 tttatttaaa atattttcaa agg
//
LOCUS       DAMJVM010000219          551 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.224, whole genome shotgun sequence.
ACCESSION   DAMJVM010000219 DAMJVM010000000
VERSION     DAMJVM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.224"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            48..>551
                     /locus_tag="PC523_003516"
     CDS             48..>551
                     /locus_tag="PC523_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HDF2476974.1"
                     /translation="MTKRVLSNFTLTGKNILSKFIFMIRYNFSTLILFELFYKGLALL
                     LILPSIKYIFDNLLKSLNIFYLNMDNIIKILSNPISIVLIILSVIILAFYAFFEFTSV
                     IICFNRSIKCEKIGLFELIKMSFKKSINLLYPKNILLFIFVLLIIPLVNTVLISGFIG
                     KIQIPDYI"
BASE COUNT          223 a           49 c           54 g          225 t
ORIGIN      
        1 ttaaaatatt gtataaaaat acaataaaca gatacaagga ggtaaatatg acaaagagag
       61 tattaagtaa tttcacactc acaggaaaaa atatcttaag taaattcatt tttatgataa
      121 gatataattt tagtacatta attttatttg aattatttta taaaggattg gccttacttc
      181 ttatactacc atctattaaa tatatatttg ataatttact aaaaagttta aatatatttt
      241 atctaaatat ggacaatatt ataaaaattt tgagtaatcc aatatctata gttctgatta
      301 tattatcagt tataatatta gctttttatg ccttttttga atttacttct gtaattattt
      361 gctttaatag gtcaataaaa tgtgaaaaaa taggtttatt tgaacttatt aaaatgtcat
      421 ttaagaaatc tataaattta ttatatccta aaaatatact attatttata tttgtgttat
      481 taataattcc attagtaaat acagttttaa tttcaggttt tataggaaaa atacaaatac
      541 cagattacat a
//
LOCUS       DAMJVM010000220          517 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.225, whole genome shotgun sequence.
ACCESSION   DAMJVM010000220 DAMJVM010000000
VERSION     DAMJVM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.225"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(57..425)
                     /locus_tag="PC523_003517"
     CDS             complement(57..425)
                     /locus_tag="PC523_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HDF2476975.1"
                     /translation="MTRPSIYNSQGYTCAEALIKSYNEEHNTDIPISIGSGMGFGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELMNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT          151 a           88 c           49 g          229 t
ORIGIN      
        1 aaatatcata aaaaaaacaa gtattttatc gaatgcaaaa tacttgtttc ttagaattat
       61 ttttgatttt ttagcatatc atttagagca tcatatgtga aatttattat ttcttcacaa
      121 gttactttat tttttttaag agatacacat atttctgagc tgtatttatt tcttactcta
      181 ttcattaatt ctcttgaata cttccttgct atgttttcat ctaaattact atttcgtcca
      241 tttaaatacc ctattataac aactgcagca tttacagctc cacaaacact tccaacattc
      301 attccaaacc ccatgccact tcctattgag attggtatat ctgtgttgtg ttcctcatta
      361 taagacttta ttaatgcctc tgcacaagtg tatccttggc tattatatat tgatggtctt
      421 gtcattttta tacttccttt caaattttat tttatatgtt ttactatttt aaatatgcta
      481 atggtctcta tattatatat actatttttg tatctat
//
LOCUS       DAMJVM010000221          455 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.226, whole genome shotgun sequence.
ACCESSION   DAMJVM010000221 DAMJVM010000000
VERSION     DAMJVM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.226"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            150..266
                     /gene="rrf"
                     /locus_tag="PC523_003518"
     rRNA            150..266
                     /gene="rrf"
                     /locus_tag="PC523_003518"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            272..360
                     /locus_tag="PC523_003519"
     tRNA            272..360
                     /locus_tag="PC523_003519"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:306..308,aa:Leu,seq:taa)
     gene            372..448
                     /locus_tag="PC523_003520"
     tRNA            372..448
                     /locus_tag="PC523_003520"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:406..408,aa:Ile,seq:cat)
BASE COUNT          142 a           85 c          104 g          124 t
ORIGIN      
        1 tctatattgt taaacttgtc aaaatttata gatttttaat agttttataa ggcaaaaaat
       61 cgaataaaaa aagtattgac atattaagac aaagatgtta ttataatact tgtcttagaa
      121 aaaaagttaa atgaaaattt agacaataat ctggttataa tagcagagag gatacacctg
      181 ttcccattcc gaacacagaa gttaagctct ctagcgctga tggtacttgg tgggaagctg
      241 cctgggagag taggacatag ccaggtgatg tgccgaagtg gcggaactgg cagacgcaca
      301 ggacttaaaa tcctgcggga cttacctctc gtaccggttc gattccggtc ttcggcacca
      361 caaattattg tgggccatta gctcagttgg ttagagcgcc cggctcataa ccggtaggtc
      421 tggggttcga gtccctgatg gcccaccaca ataat
//
LOCUS       DAMJVM010000222          412 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.227, whole genome shotgun sequence.
ACCESSION   DAMJVM010000222 DAMJVM010000000
VERSION     DAMJVM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.227"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            <1..>412
                     /locus_tag="PC523_003521"
     CDS             <1..>412
                     /locus_tag="PC523_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021394011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HDF2476976.1"
                     /translation="GWRIINNKKYYFNPNNAIAATHLYTINNDKYYFSYDGILQNGYI
                     TIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQNKFLTLNGK
                     KYYFDNDSKAVTGWQTIDGKKYYFNLNTAVAVTGW"
BASE COUNT          155 a           53 c           66 g          138 t
ORIGIN      
        1 ggatggagaa ttattaacaa taagaaatat tactttaatc ctaataatgc tattgctgcg
       61 actcatctat acactataaa taacgacaag tattacttta gttatgatgg gattcttcaa
      121 aatggatata ttactattga aagaaataat ttctattttg atgctaataa tgaatctaaa
      181 atggtaacag gagtatttaa aggacctaat ggatttgagt attttgcacc tgctaatact
      241 cataataata acatagaagg tcaggctata gtttaccaga acaaattctt aactttgaat
      301 ggcaaaaaat attattttga taatgactca aaagcagtta ctggatggca aactattgat
      361 ggtaaaaaat attactttaa tcttaacact gctgttgcag ttactggatg gc
//
LOCUS       DAMJVM010000223          376 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.228, whole genome shotgun sequence.
ACCESSION   DAMJVM010000223 DAMJVM010000000
VERSION     DAMJVM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.228"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            200..307
                     /locus_tag="PC523_003522"
     CDS             200..307
                     /locus_tag="PC523_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2476977.1"
                     /translation="MRRHLWNPSYFIATVSENTEEQIRKYINNQKKQVK"
     gene            309..>376
                     /locus_tag="PC523_003523"
     CDS             309..>376
                     /locus_tag="PC523_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2476978.1"
                     /translation="MVVVKKAYKFRMYPNKKQQELI"
BASE COUNT          163 a           37 c           63 g          113 t
ORIGIN      
        1 tatgtgaacg acaacctcaa aaatgataaa agttaagact tacagttctt atatatgtta
       61 tttaaaaata aatataaaac cttcaaaata cttgttttaa gtaattgcgt attttaatat
      121 tatgataggt gatgaaaaaa ggaagttact catggtaggg gatatgcata tagtatgtca
      181 aatataaaca tccagtatta tgaggagaca tctttggaat cctagttatt tcattgctac
      241 tgtatcagaa aatacagagg agcagataag aaaatatatt aataatcaga aaaaacaagt
      301 aaagtgaggt ggtagtagtg aaaaaagctt ataagtttag aatgtatcca aataaaaaac
      361 aacaagagtt aattaa
//
LOCUS       DAMJVM010000224          375 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.229, whole genome shotgun sequence.
ACCESSION   DAMJVM010000224 DAMJVM010000000
VERSION     DAMJVM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.229"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            <1..>375
                     /locus_tag="PC523_003524"
     CDS             <1..>375
                     /locus_tag="PC523_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2476979.1"
                     /translation="VLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQMIKSLGINIG
                     SIKILGLMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGITLFKKFTFIK
                     DTTAIIVGSFIYQFTIYFAMSLG"
BASE COUNT          112 a           54 c           62 g          147 t
ORIGIN      
        1 gttttagcct tagcttttgt attcatctgt aaagtccttt tagacttatt cctaaaaaca
       61 ggtcttggat acactttaaa gggtgttggt gataactctc aaatgattaa gtctctaggt
      121 ataaatatcg gttctataaa aatactagga cttatgattt caaatggact tatagcttta
      181 tctggtagtc ttatggctca gttcttagga ttttcagatg taaatatggg tataggaact
      241 ttagttcttg gaattgcctc tatcataatt ggtattacac tatttaagaa attcactttt
      301 atcaaagata ctactgcaat aattgttggc tcttttatat accaattcac tatatacttt
      361 gcaatgagtt tagga
//
LOCUS       DAMJVM010000225          365 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.230, whole genome shotgun sequence.
ACCESSION   DAMJVM010000225 DAMJVM010000000
VERSION     DAMJVM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.230"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(86..161)
                     /locus_tag="PC523_003525"
     tRNA            complement(86..161)
                     /locus_tag="PC523_003525"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(126..128),aa:Gln,seq:ttg)
     gene            complement(169..245)
                     /locus_tag="PC523_003526"
     tRNA            complement(169..245)
                     /locus_tag="PC523_003526"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(209..211),aa:Arg,seq:tct)
     gene            complement(274..357)
                     /locus_tag="PC523_003527"
     tRNA            complement(274..357)
                     /locus_tag="PC523_003527"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(321..323),aa:Leu,seq:tag)
BASE COUNT          111 a           87 c           73 g           94 t
ORIGIN      
        1 ttcggatact taaacttatt ctaaaattga atctaactac cttaaaataa aaatagctca
       61 aatatagttt agtaaataca tcatttggct ggggatgcag gactcgaacc tacgcataca
      121 tgagtcaaag tcatgtgcct taccgacttg gctaatcccc aatatatgtg gtgagcgcac
      181 agggattcga accccggaca cacgccttag aagggcgttg ctctatccag ctgagctatg
      241 cacccacaat tatatatgta tatcaaataa aaatggtgcg gatgaaggga gtcgaacccc
      301 cacgccaatg gcgctagatc ctaagtctag tgcgtctgcc aattccgcca catccgcgca
      361 ttttt
//
LOCUS       DAMJVM010000226          361 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.231, whole genome shotgun sequence.
ACCESSION   DAMJVM010000226 DAMJVM010000000
VERSION     DAMJVM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.231"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            <1..>361
                     /locus_tag="PC523_003528"
     CDS             <1..>361
                     /locus_tag="PC523_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2476980.1"
                     /translation="NPKTLNKQDLENMGLNLKDYNVTQECIIEDITSRKDVNKYLRKT
                     SAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPILL
                     VEKNNVPNSVKSELKRLN"
BASE COUNT          160 a           43 c           62 g           96 t
ORIGIN      
        1 aatcctaaaa ctttaaataa acaagaccta gaaaacatgg gattaaattt aaaagactat
       61 aatgtaacac aggaatgtat aattgaagat ataacttcta gaaaagatgt aaataaatat
      121 ttgagaaaaa cttctgcgcc tattacagaa cttacaggaa gcgatagata tgaaacagca
      181 gttaaaataa gtaaagaggg ttggaaaaat ggttcagata aggtagttat aataaatgga
      241 gatgtaagta tagatggtat tatatcaact ccactggcaa ctacatataa tgcgccaata
      301 cttttggttg aaaaaaataa tgtgcctaat agtgtaaaat cagaattaaa acgtctaaac
      361 c
//
LOCUS       DAMJVM010000227          339 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.232, whole genome shotgun sequence.
ACCESSION   DAMJVM010000227 DAMJVM010000000
VERSION     DAMJVM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.232"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            32..>339
                     /locus_tag="PC523_003529"
     CDS             32..>339
                     /locus_tag="PC523_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476981.1"
                     /translation="MNKKKNLIGMAISFLLIISIINPLQVEAETIIESSVYISSIQEN
                     CTYNNTKFKTIKSRSRHKISKSNKSKVKLKKRSIRTKKYGYQTINKYKIRNARRHNIL
                     T"
BASE COUNT          162 a           33 c           46 g           98 t
ORIGIN      
        1 agtagataaa aatttttata agggggaaaa tatgaataag aagaaaaact tgattgggat
       61 ggctatatct tttttactta taatcagcat cataaatcca ttacaagtag aagcggaaac
      121 tattattgag tctagtgtct acatttcttc aatacaagaa aattgtacat ataataatac
      181 taaatttaaa actattaaat ctagaagtag acataaaatt agtaagtcta ataaaagtaa
      241 agtaaagttg aaaaaacgaa gtataagaac taaaaaatat ggatatcaaa ctataaataa
      301 atataaaata cgaaatgcaa gaagacataa tattctaac
//
LOCUS       DAMJVM010000228          332 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.233, whole genome shotgun sequence.
ACCESSION   DAMJVM010000228 DAMJVM010000000
VERSION     DAMJVM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.233"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            1..>332
                     /locus_tag="PC523_003530"
     CDS             1..>332
                     /locus_tag="PC523_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2476982.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSTSKVSVKFKERPDADSITLKYKCYDMPLDTTLNYNQSTDSYEGTINYNKDPE
                     YLNVWELQG"
BASE COUNT          133 a           47 c           57 g           95 t
ORIGIN      
        1 atgagaagaa atacaaaatt attaacaaca gggattcttt caatggcaat cgtcgcacct
       61 acaatggcat ttgctactga atctaatgct atggagaata atgctgattt aaatataaac
      121 ttagagaaaa aaagtatcgt tttaggtagt acttcaaaag tcagtgttaa gtttaaagaa
      181 aggcctgatg cagatagcat tacattaaaa tataaatgct atgatatgcc attggataca
      241 actctaaact acaatcaatc aactgattcg tatgagggaa ctattaatta taataaagac
      301 ccagaatatc taaatgtttg ggaactacaa gg
//
LOCUS       DAMJVM010000229          322 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.234, whole genome shotgun sequence.
ACCESSION   DAMJVM010000229 DAMJVM010000000
VERSION     DAMJVM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.234"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(<1..320)
                     /locus_tag="PC523_003531"
     CDS             complement(<1..320)
                     /locus_tag="PC523_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2476983.1"
                     /translation="MSVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGASIVAF
                     SLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLRIMGK
                     SNIPL"
BASE COUNT          125 a           58 c           52 g           87 t
ORIGIN      
        1 agaggaatat ttgattttcc cattattctt aagttgatag agtataacat tcccatgact
       61 aaaataccag aaagtaagtt agatatctta aacttaatgt gaagaatccc tgtaacaaga
      121 ccagcagtac aaccacataa gatagccatt aaagttgcta ccacaggaga aataccatta
      181 gtaagagaaa atgctacgat agaagcaccc attgtgtagc taccatcagc agacatatct
      241 gggaaatcta ggattttgta agttatataa acccccagtg ccataatcga taaaattaaa
      301 ctttgcgtca ttacagatat aa
//
LOCUS       DAMJVM010000230          282 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.235, whole genome shotgun sequence.
ACCESSION   DAMJVM010000230 DAMJVM010000000
VERSION     DAMJVM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.235"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            166..282
                     /gene="rrf"
                     /locus_tag="PC523_003532"
     rRNA            166..282
                     /gene="rrf"
                     /locus_tag="PC523_003532"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT           99 a           39 c           52 g           92 t
ORIGIN      
        1 tcattaagat atatgtgcag cttttagagt gttaactcta tgtaaggtac taacttcaat
       61 taatgtagta tatatcataa aaacaattat tatttagtac tttaaaacta ttgacattta
      121 aaaataaaga tgttaatata atacttgtct tataaatagg caatatgtgg ttataatagc
      181 aaagaggata cacctgttcc cattccgaac acagaagtta agctctttag cgctgatggt
      241 acttggtggg aaactgcctg ggagagtagg acgtagccac gt
//
LOCUS       DAMJVM010000231          259 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.236, whole genome shotgun sequence.
ACCESSION   DAMJVM010000231 DAMJVM010000000
VERSION     DAMJVM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.236"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            <1..>259
                     /gene="tuf"
                     /locus_tag="PC523_003533"
     CDS             <1..>259
                     /gene="tuf"
                     /locus_tag="PC523_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791980.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDF2476984.1"
                     /translation="TKFTAEVYVLKKEEGGRHTPFFDGYRPQFYFRTTDVTGACKLPE
                     GIEMVMPGDNVTMEVDLINSIVVEEGLRFSIREGGRTVASGV"
BASE COUNT          105 a           33 c           61 g           60 t
ORIGIN      
        1 acaaagttta cagcagaagt atatgtactt aaaaaagaag aaggtggaag acatacacca
       61 ttctttgatg gatatagacc acaattttac ttcagaacaa cagacgtaac aggagcttgt
      121 aagttaccag aaggaataga gatggtaatg cctggagata acgtaacaat ggaagtagac
      181 ttaataaact caatagttgt agaagaggga ttaagattct caataagaga aggtggaaga
      241 acagtagctt caggagttg
//
LOCUS       DAMJVM010000232          249 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.237, whole genome shotgun sequence.
ACCESSION   DAMJVM010000232 DAMJVM010000000
VERSION     DAMJVM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.237"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(<1..>249)
                     /locus_tag="PC523_003534"
     rRNA            complement(<1..>249)
                     /locus_tag="PC523_003534"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           57 a           72 c           56 g           64 t
ORIGIN      
        1 gatccagccg caccttccga tacggctacc ttgttacgac ttcaccccag ttattgattc
       61 caccttcgac gacttcttcc aaaaggttag ataatcggct tcgggcgtct ccaactcccg
      121 tggtgtgacg ggcggtgtgt acaagacccg ggaacgcatt caccgcagca ttctgatctg
      181 cgattactag taactccagc ttcatgtagg cgagtttcag cctacaatcc gaactgagag
      241 tagctttaa
//
LOCUS       DAMJVM010000233          241 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.238, whole genome shotgun sequence.
ACCESSION   DAMJVM010000233 DAMJVM010000000
VERSION     DAMJVM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.238"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            <1..>241
                     /locus_tag="PC523_003535"
     CDS             <1..>241
                     /locus_tag="PC523_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2476985.1"
                     /translation="VLINNSSIADALSATPFAKVKNAPILLTQNNSLNQLTENEISRL
                     GAKNIYIIGGFNSIDESIENYLKDKGLNTIRISGN"
BASE COUNT          108 a           25 c           34 g           74 t
ORIGIN      
        1 tagtacttat aaataattct agtatagcag atgcattatc agctacacca tttgctaaag
       61 ttaaaaatgc accaatatta cttactcaaa ataatagttt gaatcaattg acagaaaatg
      121 aaataagtag attaggtgca aaaaacatat acataattgg tggatttaat tctatagatg
      181 aaagtattga aaattatcta aaagataaag gtttaaatac aataagaata tctggaaatg
      241 a
//
LOCUS       DAMJVM010000234          221 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.denovo.240, whole genome shotgun sequence.
ACCESSION   DAMJVM010000234 DAMJVM010000000
VERSION     DAMJVM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.denovo.240"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            <1..>221
                     /locus_tag="PC523_003536"
     CDS             <1..>221
                     /locus_tag="PC523_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2476986.1"
                     /translation="VNGEKGLADAVSMGAISAKEKMPILLTNQNDDMKEIEELIDNKD
                     ISKSYIVGGESLFNKDIEDKLPSVIKISG"
BASE COUNT           98 a           21 c           42 g           60 t
ORIGIN      
        1 gtaaatggag aaaaaggtct tgctgatgca gtaagtatgg gagctatatc agcaaaagaa
       61 aaaatgccaa ttcttcttac taatcaaaat gatgatatga aggaaataga agaactaatt
      121 gataataaag atatatcaaa atcatatata gttggtggag agagtttatt caataaagat
      181 atagaagata aacttccatc tgtaattaaa atatcaggag a
//
LOCUS       DAMJVM010000235        32101 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.guided.039, whole genome shotgun sequence.
ACCESSION   DAMJVM010000235 DAMJVM010000000
VERSION     DAMJVM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32101
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.guided.039"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            complement(327..1094)
                     /locus_tag="PC523_003537"
     CDS             complement(327..1094)
                     /locus_tag="PC523_003537"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDF2476987.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKEDIIKEAKELKGVCLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYISDIPLLFTFRSLAEGGEKVISKDYYTTLN
                     KEISSTGLVDLIDVELFMGDEVIDEVINFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQDEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELSSVLNLLHKSIN"
     gene            1855..2487
                     /locus_tag="PC523_003538"
     CDS             1855..2487
                     /locus_tag="PC523_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDF2476988.1"
                     /translation="MTIPNSTKVYPRNNDYQTVYLKNVITRENIEVGDYTIYNDFCND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHTLKSKSTYTFPIFYEEW
                     GLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVHIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNNTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDELV"
     gene            complement(2860..3729)
                     /locus_tag="PC523_003539"
     CDS             complement(2860..3729)
                     /locus_tag="PC523_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2476989.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELIELRKLGVKMIYLGLESGSNEVLEDIKKGFNSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGKISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(3825..4682)
                     /locus_tag="PC523_003540"
     CDS             complement(3825..4682)
                     /locus_tag="PC523_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2476990.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKKNNRKFN
                     IKNKIFKGIGILFVLILLSFFIWINKTYEPQKLAKEALISNSKVEVTVKENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVVASNFARDNKLIDGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNLS"
     gene            complement(4897..6915)
                     /locus_tag="PC523_003541"
     CDS             complement(4897..6915)
                     /locus_tag="PC523_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2476991.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYIFSYDLK
                     RAVYVPFGLMYIFMVSIPVGISEFPMRLSALAAGAVIIMIAQFVMNRNRMKNIGDKEL
                     ISICDELLEKISLLKNIINDDSSISKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERINLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEVNYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKI
                     KSRDEKKAERKSNIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIDMYKGIVKNIIDEVNKSIENVADVYYIKNLLLVPSLIEDRIMLINAI
                     YKDKYQEDFLENQKLLISNMYNLYINVNKNKIQDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKTLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEVA"
     gene            complement(7187..7963)
                     /locus_tag="PC523_003542"
     CDS             complement(7187..7963)
                     /locus_tag="PC523_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF2476992.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALILGLIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     VLCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSIANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            complement(8020..8991)
                     /gene="asrC"
                     /locus_tag="PC523_003543"
     CDS             complement(8020..8991)
                     /gene="asrC"
                     /locus_tag="PC523_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HDF2476993.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLTIVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTKFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDGDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDENSIIKIILNTYKYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGVKYL"
     gene            complement(9004..9795)
                     /gene="asrB"
                     /locus_tag="PC523_003544"
     CDS             complement(9004..9795)
                     /gene="asrB"
                     /locus_tag="PC523_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HDF2476994.1"
                     /translation="MNPYIPVNAKILDIVKHTDKEWTFRVNCDTKGVLPGKFYEISIP
                     KYGESPISVSGYGENYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIAGFKSPKDVLFADDLKRWSKKLDI
                     LVTVDGADEGYSGNIGLVTKYIPELDIKNINNTSIVVVGPPMMMKFTVGEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDNTYICLDGPVFDYSYAKNLID"
     gene            complement(9796..10803)
                     /gene="asrA"
                     /locus_tag="PC523_003545"
     CDS             complement(9796..10803)
                     /gene="asrA"
                     /locus_tag="PC523_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HDF2476995.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVSIPFKGTFSDTDVIRYK
                     EVNSFEEMEFTKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIREKVKFVLVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEIFNGETTEFNIDYVTDNLISVDVPENIDSNEMIRNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVTSKEE
                     I"
     gene            complement(11194..11883)
                     /locus_tag="PC523_003546"
     CDS             complement(11194..11883)
                     /locus_tag="PC523_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2476996.1"
                     /translation="MSKLKDYNIDKVMIFNKLSTKTKNQLKEKAKLKRLQKKEILFYE
                     KDSLDSVYMLLEGKVSIFKISENGERKVIFILNSGEVINDITFDSSKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQSDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
     gene            complement(11946..12794)
                     /locus_tag="PC523_003547"
     CDS             complement(11946..12794)
                     /locus_tag="PC523_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2476997.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            complement(12813..13700)
                     /locus_tag="PC523_003548"
     CDS             complement(12813..13700)
                     /locus_tag="PC523_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2476998.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGLIIDKSSLDTEAKEKGGEGIL
                     SKIKGIVKKYIKENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEIEKEF
                     NIKCEVENDVNCAGLGEMWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNINGSSFQELASTSSLIKKVAKIKNLNENELNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            complement(13758..14561)
                     /locus_tag="PC523_003549"
     CDS             complement(13758..14561)
                     /locus_tag="PC523_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2476999.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESVIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NAGCATLSKHYNIENKYVIWIQGESDAKYGSDALYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACNECDILCVGSKIAMQFHDKGLTIDDVHYTQE
                     GLNILGKDLCKNIYKYHTTKEKELLSDTIDLSEARRYIYELEKISKKFV"
     gene            complement(14721..15173)
                     /locus_tag="PC523_003550"
     CDS             complement(14721..15173)
                     /locus_tag="PC523_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDF2477000.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEEGDFL
                     PLDGKNNGYVELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            complement(15303..16805)
                     /locus_tag="PC523_003551"
     CDS             complement(15303..16805)
                     /locus_tag="PC523_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDF2477001.1"
                     /translation="MVGFTWIDFTILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIVVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YCMPEVTSWAYSLITITTSVVVGTIFSAIEAKVKAKVSLPRAETTVYYEK"
     gene            complement(16838..17704)
                     /locus_tag="PC523_003552"
     CDS             complement(16838..17704)
                     /locus_tag="PC523_003552"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDF2477002.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            complement(17789..18460)
                     /locus_tag="PC523_003553"
     CDS             complement(17789..18460)
                     /locus_tag="PC523_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDF2477003.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCISTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKRVFELGVYSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            complement(18736..19227)
                     /locus_tag="PC523_003554"
     CDS             complement(18736..19227)
                     /locus_tag="PC523_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2477004.1"
                     /translation="MEGNINNKVDDKFSKVKKKFGNGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSIDDNSFDKFCDELLELDKLIYEKNMEINEMEMVESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIVCGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            complement(19249..20613)
                     /locus_tag="PC523_003555"
     CDS             complement(19249..20613)
                     /locus_tag="PC523_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2477005.1"
                     /translation="MGEKKINNMNYSHNNYSKTTGEPTRKIKNSHITLKNKEEKICSS
                     CNKENNTKDNYCKFCGNELYEITSLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKSVLANSIGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIKTFIL
                     GYVLVLIILFVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIKPIVVFSIYY
                     AFLMGMLALFSTFILDSNINFFNTTSYGATLIMQFKVLQAIVISFIYSFVISLIGYKL
                     NSAD"
     gene            21032..22207
                     /locus_tag="PC523_003556"
     CDS             21032..22207
                     /locus_tag="PC523_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2477006.1"
                     /translation="MNLEKIKKFWGFPLDKKHKENVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFATPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKLGESVIVFGGCFLF
                     LNFFLPGIQTIPGNEFSNIVNTFFITSLAVFVSTMLYRSRVFTYYDKLLISQQYEEIY
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCDEEDAKLKSECLRKII
                     ETCNFERDDIPLKCVTVSIGVYTNKIERTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            complement(22380..23777)
                     /gene="asnS"
                     /locus_tag="PC523_003557"
     CDS             complement(22380..23777)
                     /gene="asnS"
                     /locus_tag="PC523_003557"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDF2477007.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFVRITYTKAVELLIESGHDFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     misc_binding    complement(23854..24087)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(24245..24439)
                     /locus_tag="PC523_003558"
     CDS             complement(24245..24439)
                     /locus_tag="PC523_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HDF2477008.1"
                     /translation="MENKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVTEAKDWVDNGSKL"
     gene            complement(24691..26364)
                     /locus_tag="PC523_003559"
     CDS             complement(24691..26364)
                     /locus_tag="PC523_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDF2477009.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPIDFDET
                     ILNNIIQVAWWEESAPMSSLIEITNNVDNGETITTAAGTEYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILAGNGRVNSKYRGITTESDLIVVKLKSYIGTYYAGRINYSV
                     SDFLAAITYVTNIARTENRPLIINLTIGVKSGAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYAPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWTLRLTSELI
                     IGGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVAPGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQNNI
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            complement(26374..29772)
                     /locus_tag="PC523_003560"
     CDS             complement(26374..29772)
                     /locus_tag="PC523_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF2477010.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNTLLTYPEIEFIEKPFVLQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSNGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELKMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLRNNITEEVEF
                     IVGEGERILNVNIWPDFVDDFSVHLVNPSNNQTQTISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWRIIFEPINIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKVARRLSNQSY
                     PNNSSGFGFLNLSDVDLYTLSSINQDLETEDIGYRSINKSFMDEANNYIPIDGYNVQI
                     HNGLEKEKYISKNISRQSGILSGIDVVHTPEFEEELAGLGQSQNFLKISDSLGVLSID
                     NTDYNSIQQVLQLPSIIRTIPTTKMTLLGEINRGTFGGVVATEEIGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHRDFIYPDGTSKIVYLWDQTKEGNPPDGFYIGTEYTREDIN
                     RAIAANDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGVAEDSELIVIKLGKIEGFYNS
                     AMLFAASQYAYNKAFELGRPLVINVSLGTSSLAGLTNRVNSEKAFFTRGLCVTAGAGN
                     EGNTQTHTSGIISHVGGSVEVELELNEEEEELSLELWLSRPDKADVIIVSPTGEESKT
                     VGVSNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRKLLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINSSLWQSSSRG
                     PTIENRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            complement(29938..31134)
                     /locus_tag="PC523_003561"
     CDS             complement(29938..31134)
                     /locus_tag="PC523_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDF2477011.1"
                     /translation="MFIEFFYLLRSRGVNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDTVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWVLKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFRRKYSKTVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            complement(31155..31976)
                     /locus_tag="PC523_003562"
     CDS             complement(31155..31976)
                     /locus_tag="PC523_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDF2477012.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKNLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGIPIEKIEKEVPFAGILLKNNEDIESMQRHL"
BASE COUNT        10538 a         5608 c         3245 g        12710 t
ORIGIN      
        1 aaaaagtaat tacaaataca cataatacaa atatatcttc tactttagat aaaacagtta
       61 ttataaaaac ttactatcta taaataatag aaatgctagt ataccatcta taatccaatg
      121 tctagtaggt tttaatggca tattttaaat atgccatagt tgatttataa gatttttata
      181 ccactttcaa aatacacact aaatagttgc ttttcagata ttacacttaa ttaaccaatt
      241 taaattttac tatctgaaaa gttttttata tttaatttat atatcttcaa aaataactcc
      301 taatatttct atataattat attaaattaa ttaatactct tatgtaataa attaagtact
      361 gagcttaact ctttaaatga tatttgacct ggtgctgaaa cactttttgc agctccaaaa
      421 gttaatgcag aaccaaatat ttccccacac aatctgctta taactcccat tcccgacatt
      481 gacattgtta ttattggtct atctgcataa attttaaaca tttcatttgt agcttcaagt
      541 agaactaaca catctttttc atcttgtggc ataacagcta ttttgggtaa atctgcgcct
      601 aattcctgca ttctacataa acgagataca atttcttctt tttttggagt tttattaaag
      661 tcatgattag atataattac tttcacttct tttttatgag caaaattaat tacttcatct
      721 ataacctcat ctcccataaa taactctaca tctattaaat ctactaaacc tgtactagaa
      781 atttctttat ttaaagtagt ataataatct tttgatatta ctttttctcc cccttctgct
      841 aaacttctaa atgtgaataa taaagggata tcagatatat aacttcttaa ttcatagaga
      901 acttccttaa cttctttaat attttctaca ttctcaaaaa aatcaactcg ccactctata
      961 atatccaaac atactccttt taattcttta gcctctttta taatatcttc tttattttta
     1021 ccgataatcg gtacacaaat tttaggtcta ccttctccaa tagtaatatt ttttacttga
     1081 acttttcttt tcatattctc ctccataatc agaaattcta tatgatttta aattctattt
     1141 tctgcataat ttaattatac attataaatg ctacacatta taaaaatact aatttaaaaa
     1201 taatttttat tctaattctt aaaacaatcc cacatatttt agaaaagata ctctataata
     1261 taaaaacact tctttcttat ctaaatttat tttaacattc tataattttc ataacattac
     1321 ttaagtttta tctatataat caatattatt ctggatgata atagaattaa tatataaatt
     1381 atagtataat tatatagaaa taactcgaat tttactactt atgaagaaat ttgatatcaa
     1441 taaattcaaa tcatttgcat tacttcagag ttttggctaa agttaaacac catactaaac
     1501 ccaaaataga ttttacagct atacttcaag agtaattgat tgtaatagta ccatataatc
     1561 atcctttagc attaagtgac agtgtagatt aaaaggataa caatgttaat gttctttaaa
     1621 ttaaaaatta ttttgtatat aaaatatatt aaattattgc aagctttatt gttttgatat
     1681 acaatcaact aagcatatag cctttataaa atactaaata gttaaaagtc tgtttttaaa
     1741 aacaaaatgt ataaattaaa atatagatac tatcaaggag tgattctgtg aagagatgta
     1801 ttgtaatagt ggaatagtca aaaacataat atatatcaaa ggagagattt aaatatgact
     1861 attccaaatt caactaaggt atatccaaga aataatgatt atcaaactgt atatcttaaa
     1921 aatgtaataa caagagaaaa tatagaagtt ggagattaca caatatataa tgacttttgt
     1981 aatgacccaa gagattttga gaaaaacaac gtattatatc attaccctat taacaatgat
     2041 aaattaataa ttggtaagtt ttgctcaata gcatgcaatg caaaatttct aatgactagt
     2101 ggaaatcaca cactgaaatc aaaatccaca tatacctttc ctatctttta tgaagaatgg
     2161 ggactcgata tcaatcatat aacggatgct tgggacaata aaggtaatat tataattgga
     2221 aatgatgtgt ggataggcta tgattctata attatgtcag gtgtacatat tggcgatggt
     2281 gcaattattg gaacaagagc agttgttact aatgatgtcc caccatattc tattgttgga
     2341 ggagtacctg caaagattat aaaaaaaaga tttaataata atacaatttc aaaattattg
     2401 gaaattaaat ggtgggattg gccatataaa aaaatccaat caaacattaa gcatattcaa
     2461 tcaggaaata tagatgaatt agtttaatct tttataattt atatatatta aattttactt
     2521 taataatcct ttttataaga attttttcaa gatttgtata tcattttaat atatataaat
     2581 ttaatacaat actaaacaaa aataaaaagt aaagttgctt aatataaagt tactttactt
     2641 tttattattt aaattcatgg gtccattata atatgtaaaa ttctattaaa ccagttcaca
     2701 ttttcatcat tattgatagc ctatatatga acataccatt ttgtttttta acaaaaaaat
     2761 catgctatat taatagtaaa aaactattaa tatagcatga ttttttgtat ctaaacaatc
     2821 tcagtattta tctataaata tagattgata tatcaaaaac tacagtcttc tatattcttc
     2881 tctttttaac ttttggttac ttagatagta atcaatttca tcaattagac gttgtttgtc
     2941 atcaggtaat acacctttta aattagcata atttgatgca tgattacttc taaatacaat
     3001 tttttcattg gcatctatat tctttatcat ctctttaatt tcttgtaaca cagccttatc
     3061 ctctagtatt gtaaactctc ctgccttaag taaatcgtaa agttcagtat taggttctac
     3121 cattaaacta agtacaccta cataatctgg tgaaattttt cctaacattt ttcctgtatc
     3181 aacggcatgc tgatgtgttt ttttagttcc tccaagacct gctattacag tggctgataa
     3241 tttaatccca ctttttttaa cttttctacc aacttttatt aattgttcac tattaaaacc
     3301 tttcttaata tcttccaata cttcattgct tccactttca agccctaaat aaatcatttt
     3361 aacaccaagt tttcttaact ctataagctc atcatcagat tttttctcta tagatttagg
     3421 agaaccatac atagtaattc tttcacactc tggaaaaact tcttttatat ataaaattat
     3481 gtctcttaat ttctccattg gtattatcaa tgcatctcca tctgctaaga atattctttc
     3541 tacatgttta taatatcttc taaatatatc aatttcattt tttattactt ctagtggctt
     3601 tattttaaat tttttagatt tgtacatact acaaaacgta catttgttgt gagaacatcc
     3661 tagtgtgact tgtattataa gactataagc ctcacttggc ggtctgtaaa gtggcatatc
     3721 gtattccata ttaaaatccc ccttgtaata aaatatttat tctattatat tattaatatt
     3781 tactttaaat aataacttat aaaattatat tttttaattt tagtttaaga taaattcttt
     3841 aaaaatttaa tgatactatt ggcagtaata ttaagttgtt tttcttgact tataatagca
     3901 tcattatctc ccttttgttt tccatagtct ccaaattgtg catgatttcc tccttcaatt
     3961 tcaacataag ttgtatcatc aggaagtttt tgttttgatt ctacaagact tttaaaattg
     4021 actacaccat ctttgctccc ccatatagat ataacttttt ttcctaattc tttcaactca
     4081 tctcccattg gataagaagc taaaaataca actccatcaa ttaatttatt atcacgtgca
     4141 aaatttgatg ctacaactcc tcctaatgaa tgacctccta ttacccaatg ttctatgttg
     4201 ttgtaagaat ccataacttt ctgcgcctta ttagttccaa gtatagctag attaagtggc
     4261 atctttacaa ttacaacctc atatccactt tctgctattt ttcttgcaag tggtgcataa
     4321 gattctattt ctactttagc tcctggatac aaaattaaac ctttagaaac ttttcttcct
     4381 tttggtgtaa atgaaatgtt ttcttttaca gttacttcaa ctttactatt gctgattaga
     4441 gcttcttttg ccagtttttg aggttcatat gttttattta tccatatgaa aaaactaagc
     4501 aaaatcaata caaatagtat acctattccc ttaaatatct tattctttat gttaaatttt
     4561 ctattattct ttttatcatc tttgttttca acctcatcat aaagtaaagg tctttcgagt
     4621 tccatagtta tactaaatgt atcatatttc ttattgcgtt ttacctttac atctttactc
     4681 ataaatctaa cccccataat atttgaatac tattatagtt tatcctaagt aaattgtttt
     4741 ttcaaccaat gaaaaaaatc aatattttaa ctattttcat ataaaattca taaatatatg
     4801 ttttttgttt tttatatatt attttctttt aatattttat aaaacaaata gccatatact
     4861 ttattttaaa tatatggcta ttctataatt taatatctaa gctacttctt ttattgaaac
     4921 tttagttata ttacttattc tatacatttc tttgacacca tttagagttt gcagtaagtt
     4981 tagacatatt atcttgtctc ctagagtttt agtatttaca atactctcta aaattacgct
     5041 tcttccctca tcatacttat ctacattgta atttgaaata taattagtat ctctcaatat
     5101 tttttcaaca tcttcatctt gtattttatt tttattaaca tttatatata gattatacat
     5161 attacttatt agtaatttct gattttctaa aaaatcttct tgatatttat ctttatatat
     5221 agcatttatc aacataattc tatcttcaat aagtgatggt accaataata aattctttat
     5281 atagtaaaca tcagctacat tttctataga cttattgact tcatcaatta tatttttaac
     5341 gattccttta tacatgtcta tcacatgttg atagcctgtt ttagcatcat atggtaatat
     5401 aaacttattt actattaatg caataattgc tcctaatgct acatatgaaa tcctgcttat
     5461 tgttaaaaca tttgggtctc ccataactac agctgaacca agagctgaaa cagttacaca
     5521 taccattaat tttctataat caactacata agagtttata tatcctgcta aaagaactat
     5581 taacgacctt aatgtactat cttttattat tgaaaataat acaataaata ttacagctcc
     5641 tataaatgta ccttctattc tttttctaga cctttgtata caattttctg cgtaaggttg
     5701 tgttaatgaa aaaacagtta acattatcca tcttccatcc cttaaatgga aataatccat
     5761 aataaaaccc gctacagctg tagctaaagc aatctttact gcataagaaa atcttataga
     5821 atttaaatta aagtttttct tatatattga tagtcgttta aattcatgag gtatatttga
     5881 ttttctttca gctttctttt cgtctcttga ttttattttt ttataatcta ctaaaaactc
     5941 atataaattt tctaaaacac tataaatttc ttttaaattt atatttttag tatcctgagc
     6001 aataacataa tctctaaatc ctaatatctc tttatcagtt atggtatctt tattttctaa
     6061 acacatctta atatagttta cttcttttgt tgctacaact agaacatctg atttactatt
     6121 tatgctactt tcttctaaaa tattttttat atcttcatct tcaaattctt tactatcctt
     6181 tttgtatctt tctaggatta aatttattct ttctaatgaa aataaaatat tcattatgtc
     6241 tatacctcta atacttatat aaaagtcatc ttttctatta tcaaaaataa ccattttcaa
     6301 atttttagat atagaattta ttctataatt acagctatct atttttctca tactagattt
     6361 acttatagaa ctatcatcat taataatatt ttttaaaaga ctaatttttt ctaataattc
     6421 atcacatata gatatcagtt ctttatctcc aatatttttc atcctatttc tgttcataac
     6481 aaattgagca atcattatta taactgctcc agcagctaaa gccgacaatc tcataggaaa
     6541 ttcacttatt cctacaggta tacttaccat aaatatatac attaggccaa aaggtacata
     6601 tactgctctt tttaagtcat aactaaatat ataccctatt ataaataatg ctataaaatt
     6661 taataaaacg cctaaataca tattttgtac tgcaaaaaat gatagtattc ctaggattat
     6721 atttattgct aaatacttta gtaaattttt aattggagaa atagttaaat ctctttccaa
     6781 tagtgttagc atagctgtta ctataccaac acctataagt gtgtttttac ttccaaataa
     6841 atatatgaat atagaaataa atgctattat aaaaataaac aattttgttt ttgatattac
     6901 tagtttcttt gtcattatat cccccctaaa aatttcaaca agaccaattt tatcatattt
     6961 gttatcttat tcaaaatact cttttttacg gtaggacttt aacttttttt acattttgaa
     7021 ctatataatc tatatttaaa cgtattttat tgcatatata gtttatatat tttcgtatgc
     7081 tcccatatat tttttctttc aaatattttt tcaaaaatat aaaaataact ctctcaacaa
     7141 tactaaaact gtatttatta agagagttat aatttataca ataaatttaa ttaagctttt
     7201 ctttacccaa tatataagtt ccaagaccca taaataaagc tcctccaaca atatttccaa
     7261 gtgtcacagc tactaaatta taaatagctc ctccaatagt aactgtactt attgttggtg
     7321 aaaataatga aacactataa attgtcatat ttgctatact atgctcaaaa cctgatgtta
     7381 taaaggcaaa taaacataag aatatcataa ttatttttgc agtatcacta ttagttctaa
     7441 acgaacacaa tactgataca cataccagaa tattacataa aacccctcta aagaaaagag
     7501 ctgtaaatgg catggatgct ttactagctg ctgtatttgc aaaaaattca gctacaggtc
     7561 ctttatctac tagaccagtt cctacaaata taagacctaa aattaaagct ccaattaaat
     7621 tacctaccca actgtatgcc catattttag aagtatcttt tatacttaca cctttattta
     7681 acataccagc agacataacc atattgtttc cagtaaataa ctctgttcct gtcattataa
     7741 ctaaacttag tgctattgca aaggataacc ccattactat cttagtcata ggtgaacctg
     7801 catcagtcaa aagtcctcca atagtaaata tcaaaagtat acctattcct acataaagtc
     7861 ctgcaaacgc agaagatacc aaatatttta ctttacttgt gtttaataaa tttatcttat
     7921 ttatagctgc attagttaat ttatccaaag tttctttatg cataattccc tcttccttct
     7981 attaaatttt aatttattca aatatatgtt ttactcaatt tatagatatt taactccact
     8041 ccagtatatg ttttcataca ttttagttat ttctggaagt tctacacctt caagcgccca
     8101 ttttttaaac tccataaatc ctgttctatc aattatatat cctatatgtt cttttcctcc
     8161 tggtgcatct ttatctatat acttttctac atatttgtaa gtattaagta ttatctttat
     8221 tatgctattc tcatctaccc ataaaagaaa atcttctgct agtctagggt ttttctttcc
     8281 tgttctaccc attatagcta atctatagta ttttttctca tctctagtcc atgcatttgt
     8341 tgggcaattt agtacacatt ctccacatcc tatacattta tctccatctc ttaccacctt
     8401 attattttct gtttttaatg ctccaacaga tattttagaa cattttttta cacacgctcc
     8461 acaatttaca catctatctt tttcatatat gggtaaagcc ataccaataa ttccaaaatc
     8521 attcattcta gcttttatac aatcatttgg acagccagtt aaagccactt taaaatgtaa
     8581 atcatttgga aatatagctt tttctattct tttagcaaat ttagttgtat tatattgagc
     8641 ctttggacag actttatttc ctatacatgc tgctatattt cttgtacccg ctgctggata
     8701 acctgcattt ttctcacttt gatttatatt cattttttca ataacaggtt gtataatttt
     8761 atttatttct tccatatctt ccatatttat tcctaatatt tcaaagccct gtcttgtagt
     8821 tatgtgtacc tgtccattac catactcact tgcaatttta gaaactatag ttaaactctc
     8881 agggtcaata agcccaccag gtactctaac ccttaaagaa gtctcatatt ttgtttttgt
     8941 aatcctataa gcatttttca ttactttttt ggtatttaaa tctcttatca caaacttcgc
     9001 cccctaatca ataagatttt tagcatatga ataatcaaat actggtccat ctaaacatat
     9061 ataagtgtta tccattttac agtgtccaca tttacctata ccacaacaca tttttctttc
     9121 ataagaaacc caaatattct tttcatctaa atctcttttt aaaaattctc ctactgtaaa
     9181 cttcatcatc attggaggtc caactacaac tatagaagta ttatttatat tttttatatc
     9241 caattctggt atgtattttg tcactaatcc tatatttcca ctatatcctt cgtctgctcc
     9301 atctacagta actaaaatat caagtttttt actccatctc ttcaaatcat ctgcaaataa
     9361 aacatctttt ggtgatttaa atcctgctat taatttaaac ttttcgcatt tttctttatt
     9421 gttgtaaaaa tattcaacta ttcctcttac aggggctaat gcacttcctc ctgccactac
     9481 tacaatttct cttccttcgt aaagagatac atcaaatcca tttccataag gccctcttat
     9541 aaaaaaagaa tctccttctt tataattaaa caactcacta gttacttttc ctacatttct
     9601 tattgtaaag tcaatatagt tctctccata tcctgatact gatattgggc tttcaccgta
     9661 tttaggtata gatatctcat aaaattttcc aggcaataca ccttttgtat cgcaatttac
     9721 tctaaaagtc cattctttat cagtatgctt aactatatct aatatttttg cgtttactgg
     9781 tatatatggg ttcatttata tttcctcctt actagttact aaatcattta ttttttctat
     9841 acattttgaa aatgatatgt attgtggaca ggcatcatca catcttccac aaccaacaca
     9901 catatgatat ccgaatcttt ttttaaaatc atgtatttta tgcatagttt taaatctcat
     9961 tctttcacct tgtttttttc taaaagaatt tccacctgct atatctgtat atccatctac
    10021 ttgacaagaa gcccaaactc ttcttctctc acctacattc tcattttcct tatagaaaat
    10081 atcctgcatt gtaaaacatg aacatgttgg acatacaaaa ttacatctac cacatcctat
    10141 acatcttgta tcatattcat cccatataga atttcttatc atttcatttg aatcaatatt
    10201 ttctggcaca tctacagata ttaaattatc agttacataa tcaatattaa attcagtcgt
    10261 ttctccatta aatatttcaa attcactatc ctttatgtct aaatacagtg tatctccttc
    10321 tatatttaat ccaattgaat aattatcagt tttgttagaa tccatactca cacaaaaaca
    10381 gtttctaaaa ctttccttac accctacaag tacaaacttt actttctctc tgatattttt
    10441 ataaaaataa tctttttctt cgccattatt tagatatatt tcatcaagtc tttttatccc
    10501 atttatgtca caagctctca agaaaattaa aattttcttg ttgtctaaat cgctttcctt
    10561 aaactctttt tcagtaaaat aaaataatac ttgatttata ggaagtacaa cttcttttgg
    10621 tgagaaattt gctttcttgg taaattccat ttcttcaaaa gagtttacct ccttgtatct
    10681 tataacatca gtatctgaaa aagtaccttt aaatggtatt gaaactggtg caaatatctt
    10741 gtagtctttt tttagataat tcagaccttc attgaattta gatgaatcta attttaattt
    10801 cattcttatc acctttctta tattttagtt ttacttactt cttatcttgt ttaaatctta
    10861 acattttatt ttctatgctt ctgtgataat tatcacagtt tcaaaaataa taaaaaaata
    10921 ttaatagagt ttattaagct ctattaatat ttttttatta tctatattat tcgattacaa
    10981 atctagtcac ttaaatacaa tcattaaata caataaatct attatctact caactttttg
    11041 aataatagag agtcttattt gcttttttat acaatttagg aaaggtaaaa taaggattaa
    11101 taaattagac aataaaaaag aatctataaa tagattctcc caataatatt tttaattaat
    11161 taaaatccta ataataccct tatttaatca taactaatta tttttaaaat accttgataa
    11221 ttcagtttct ttaacgtaaa aacctttatt atcaatctta ataagttctt ctttttgtaa
    11281 tacctttatc attctagaaa cagtctctct tttacagcca agcatatcag ctaaatatgt
    11341 aactgtaata tttacattta gtaaagtcca ttctccacag caaactccaa attctctact
    11401 taacctatat aattttgctg cgagtttttt atctactttt atagatatag aatttttcaa
    11461 ctgtctatat aaacgtctta ttcttctttc catataagaa ataatatctt ttgtaagttg
    11521 aaaatcactt tgcataattt ctaaaaactc ttcaatacta taataagcta caattgagtt
    11581 ttcaaatgct tcacagccaa cagtggagtt tttagaagag tcaaatgtaa tatcatttat
    11641 gacttctcca ctatttaata tgaaaattac ttttctctca ccattttcac ttatcttaaa
    11701 aatagatacc tttccttcta aaagcatata aacactgtct aagctatctt tttcataaaa
    11761 taaaatttct tttttttgta atctttttag tttagctttt tctttaagtt gattcttcgt
    11821 tttggtactt aacttattaa aaatcattac tttatcaata ttataatctt ttaatttaga
    11881 catgtaatat ctctccattc ctaagttttt aagagagctc tataaaacat tacaaatttc
    11941 ttattttatt catttttaaa caattttata gcatttgtag ttttaatttt tctttcaata
    12001 gcttcattag aaagttcttt tactacttgt gtatatacta aatcaataat gaagaattgt
    12061 gctaatttag aagatattga accggtttcc agtactgttt ctcctgatac ataagataag
    12121 tgtacatctg aaatatcctt taattcagaa tttttatttt cagtaattga tataatttta
    12181 gcattattgg ctcttgcaag ctttacagtt tttataatct cctctgtttc accagaatgt
    12241 gaaattgcaa ttattaaatc tccttcttcc attatggaag acatcattat catggtatga
    12301 ctactgtcaa aacttacaca attaagacct attctcataa atttatagtt tgaatcctgt
    12361 gctattatac cagaatatcc aatacctatg aagtaaactt tctttgcatt tattataagc
    12421 cttgcacagt catgtacatc cttactattg attatctcca ctgtgttctc taaagtggtt
    12481 acatttgaac ttaaaagttt ttttgctgta tctaaagctg gctcatcatt ttgtatattt
    12541 ttatttataa tatttttttt attgatagtt gatatttctt gtgctaaagt aactttaaaa
    12601 tcctgtaatc cattaaaatt catttttttt acaaatctag ttatggttgc ttcaccaata
    12661 ttgctttctt tagctatttg tgatattggt ttataaaaca catcatctat attttctttt
    12721 atataagcta ttaataattt atctgacttt gtgactttaa aagttggtga ttccaattgt
    12781 tctaaaatac tcattaaaaa cccttctatt ctttatattt tgtttaaaaa attatataat
    12841 gccccaagca ttcccgcatc attttgtctc tttgcaaatt ctatattagt gtttttgtaa
    12901 actctctcta taagaacctc ctttaatgct ttgtctattt ttggttttag gaacttttct
    12961 tgtgccataa tcccaccacc taaaattact acctctggat ttattacata acatatattt
    13021 gcgattccaa ctgctaatga ttttatcata ttatcgagtt cttttaaaca atcttcgtca
    13081 ttattctttg ctaaatcaaa aattattttt ccatttaatt cattttcatt caaatttttt
    13141 atttttgcta cttttttaat tagactgcta gtagatgcca attcttgaaa agaacttcca
    13201 tttatattca tatatcctac ttcgccagca ctattactaa acccatttat cagtttattg
    13261 tttattataa tacatccacc aattccagta cctattgtta aacatataga agatgttgca
    13321 cctctacctg ctccaagcca catttcacca agtccagcac aattaacatc attttcaact
    13381 tcacatttta tattaaactc tttttctatt tcttttttta attgcatacc cttatagttt
    13441 ggaattaatt cttctaatgc aaatagtatt ttaccttcaa ctgggtcaac catacctgca
    13501 gttgatatgc atataccact tatttcattt tcttttatat acttttttac tatacctttt
    13561 attttagata atattccttc tccacctttt tcttttgctt cagtatcaag acttgattta
    13621 tcaattatta atccatcttc tctaataaat ccatatttta ttgaagtacc tccaacatct
    13681 atacaaacgt aatttttcat aacatatctc catataaatt atttttataa attgataaca
    13741 ctacttaaac tcttatatta aacaaatttt ttacttatct tttccaattc atatatatac
    13801 cttcttgctt ctgataaatc tattgtatcg cttaataatt ctttttcctt agttgtatga
    13861 tatttataaa tatttttaca caagtctttt cctaatatat taagaccttc ttgagtgtaa
    13921 tgtacatcat caatagttaa ccctttatca tgaaattgca tagcaatttt gcttcctaca
    13981 cataatatat cacattcatt acaagcagac tcctgtgctg ctgcaattcg tcttgttcta
    14041 ataaaatatt catcttctcc aagccaataa ccagttaaac ttacaaacat tttatcaatc
    14101 atacattctt ctttaagtct ataagcaatc ttttttagac tttctttata gtacaatgca
    14161 tcactaccat atttcgcatc actttcacct tgaatccaaa taacatattt attttctata
    14221 ttataatgtt tacttaaggt agcacagcct gcattaaatt tttctatagt gtcatttagg
    14281 aaccagttat tatcatgatt ccagtttttt attcttgaac catcttttgc attgtgaata
    14341 aaacaaactt tatttccagt aagcttattc cattcatttg aaaatgcagg tgctatagta
    14401 ccatcacctt ctgaagtttc tagctcacat ctcatattta taatttcatc agtacttaaa
    14461 tattcaaaac aattttcatt tggaatgaca ctctctttgg gattccctaa tcctctagca
    14521 ttgctttgtc ctattaataa gaatacatct atattttcca taagttaact cctttacaaa
    14581 attttaaact tttaattatt ttcaatttcc ttataaaaaa ctaaggctga aaatagtaat
    14641 ctaactaaaa tttacgaagt ctcactaatc gctgaaagtt aaatcactat tttcagccct
    14701 agtggttata taaattataa ttatctaacc aaaaccttaa aaatagcttt tttgatattt
    14761 tctttttcta aaacttttat tcctgtcata tgagcatctt ctggataaca aactaaaaaa
    14821 tcgccttttc taagttctac atatccatta tttttaccat caagaggtaa aaaatctccc
    14881 tcttcttcat atattttttg ttctaaattt tctataaaat ttatatctat tcgttcacat
    14941 ccatcaagca tcaaatgaac atctatatac ttcttatgag cttcccagaa tctatcttct
    15001 ttttcacaag ttttatactc tacaatattc acaaaaatat catcaccatc tattgtataa
    15061 gtaccttttt cataatctat taatgaatta tctttagcat attcaaaaca tctaagtaaa
    15121 tcttcatgca atgttgaata tgtcttacta tgatttatat ttccaaatat cataagcact
    15181 atctccctat ataattataa agtctatttt tatactttta tatataaagt atattataca
    15241 atcttataga gtaactaaat tatttatcta atcactctat aagacaattt atcatttaaa
    15301 tattattttt cataataaac agtagtctca gctcttggta aagatacttt tgctttaact
    15361 ttagcttcta ttgcactaaa tattgtacct accactacag aagtagttat tgttattaaa
    15421 gaatatgccc aagaagttac ttctggcata cagtatttta aataaacaac caaaattgct
    15481 gatactataa acccaacata tgctcccatc ttagttgctt ttttacaaaa tgcaccaagt
    15541 acaaatattc ctgctaagac acctagtact agacccataa aactattgaa ccattcatat
    15601 gctgacttta tttctccatt tgctaatact attgcaacaa ctatagaaag tatacctaca
    15661 cctaatgata tatattgtgc tatcttagtt tgtttttcca tacttatatc ttttgttatg
    15721 atagtttgta tatctaaagt ccaacttgta gcaactgagt taagccctgt tgataaagtt
    15781 gattgagaag ctgcatatat tgctgcaagt agtattcctg ttatacctac tggtaactca
    15841 taagctatat atgatgcaaa tacttggtct tgttgaactg tttgagctaa atctggattt
    15901 tgagtatagt atataaataa acaagttcca attaaataaa atactgttgc aagtcccatt
    15961 gataaaacac catttccata tgtcatttta ttaagttgct ttatatcagt tgttgtagta
    16021 aacctttgaa ctatatcttg actcgataca tatgatgaaa aagtattaag tccagctcca
    16081 acaaatatta taaatgcact tgtagatagt atatttttat caaacataac ttcattactt
    16141 gctaagaact tgtgtccagt tgtaagagta tccattactg ttccaaaacc accatttata
    16201 cttgctatta aatagaataa tgttcctgta acacctacta ttagtaccat tccttgtata
    16261 aaatcggtcc ataaaacagc ttttaatcca cctgtatatg agtaaattat tgctattaca
    16321 cccataacta ttatcagtac atttacaggt attcctgtaa gaccagccag aactattgat
    16381 ggaagataca ttataataga catacgtcct atttgatata ttataaacat taaagctcca
    16441 agcactctaa gacctttgct ttcgtacctc atttctaagt actcataagc tgtatcaatt
    16501 tctaacctac tatatagagg taagaaaaat tttattgtta aaggtatagc tataaacata
    16561 cctaattgtg caaaccacaa aatccaagtc ccaccataag aatttcctgc taatgataaa
    16621 aacgatattg gacttaataa tgtggcaaat attgatactg atgtaaccca ccacggaata
    16681 gttccatcac ctttgaagaa ctcttttccc ttcatttcct tttttgaaaa taacaatcct
    16741 gcaaataaaa ctgccaataa gtaaaccact agtattgtaa aatctatcca agtaaatcct
    16801 accatttgac taatcctccc atactaaaag tattttatta aaaatacttt ctgtatattt
    16861 cttttgctct tgatttcatt tcatcagttg cttctttcat aggttgtcta cagtatccag
    16921 cctcaacacc ttgttcttca agtagcaatt taattgtttg atataatcca tttcctaata
    16981 tatctgttat taaatcatta gtcacatgtt gtacttcaag tgcttctgat attttttcat
    17041 tcttagttaa ttcaaatatt tgtctagctc tgacaccgtt tacattaaat gtagaaccaa
    17101 ttgccccatc tacacctaaa actgtagctg gtaacatcat ttcatcaaat cctgcaaata
    17161 ttaatttatt tgggaatgtt tttctcattc tctctaataa gtagaaatct gctgctgtaa
    17221 acttaacacc tattattttt tcattttcaa ataattctcc aaactggtct aaactcatat
    17281 caactccagt taaaaatggt atagagtata ttattaatct attatctaca gaatttatta
    17341 ttgtattata atagtgtttt atttcttcaa aatcaaactt atagtaaaaa ggtgtaacag
    17401 cacttattgc atcatatcct aaatcagttg taaatttagc aagctctaca gcttctttta
    17461 aattgactga accaacttga gctattaatt ttatttcttc cttcacttca tctttagcta
    17521 tttcaaatat tcttttcttt tcatcagttg aaagcataaa gttttcccct gtacttcctc
    17581 ctacataaag accatctact ttacaaacat ctatattatg tctaattatt tgtcttaacc
    17641 ctttttcatt tatatttcct tccttatcaa atgaaactaa taatgctgaa taaattccct
    17701 tcatatctgt tgttctcata tctttacctc caattttaat tttcaagtat agttaaatat
    17761 aatagttttt taatttataa attaattttt aattaatttc aattgcttta acaaattttt
    17821 ctgttattaa ttgtggtctt gttattgctg aacctacaac tgatgaatag actcctagtt
    17881 caaaaaccct ttttaaatct tgaggagtat ttacctttcc ttctgctatt acaggtattt
    17941 ctaaatcttt aactagtctt tccattaata caaaatctgg accttctaac gttttagtat
    18001 atggagtata tccagatagt gtagttgaaa tacaatctac accatattct tgtgctttga
    18061 tagcctcatc ataatttgaa atatctgcca ttaccagaac accattttct tttatatact
    18121 ttataagttc ttttaagtct tcattatttg gtctaactct attagtagca tctaaagcta
    18181 tcatttcaca ccctgttgtc aaaagttcat caacttcttt tttagttggt gttatatata
    18241 tatctgaatc ttcataattt ctctttataa tacctataac aggaagtcca gtaacttttt
    18301 ttatctcaat aatatcttca actccttgag ccctaattcc aacagctcca ccttccatag
    18361 cagccttagc cattctcccc attataaaag gactatgaag aggttcattt tctaaagctt
    18421 ggcatgatac tattaatctt ccctttacct tatctagcat tttttcaatt acctcatttc
    18481 tgaaatttat tttcttgatt tatgtattaa taataacatg aaaacttttt ctttgcaata
    18541 ttttttcata tttttttctt aactctatat ttttactgat ttattctata tattagtctg
    18601 ttttttatac ttaaaagaaa ttttatttca tttttttggt gtttattttc attttgtata
    18661 accactatta tatgtattct atataaataa tttcaataaa aaaacccatg tatactatac
    18721 ataggtttcc caatattatt ctaaatccat tataattttt cttccacaac atggacaaaa
    18781 ttcagcctct gtatctatct cactttcaca ataaccacaa actataaaat catcacattc
    18841 attttccaat tcaaattttt tgccacattc agcacaatat ttatcattta catttccaac
    18901 atagccacat tcacaagtta cattactttc taccatttcc atttcattaa tttccatatt
    18961 tttttcataa attaacttat caagttctag taactcatca cagaatttat caaaactatt
    19021 atcatcaata gaatcttctc taattttttg atatgtcaat attcccattt ccaagaatat
    19081 ctttgctttc ttttcttgga tatcatcaat ctctctattt aactttgatt tttctttata
    19141 taattcaact ttaaattttc cattaccaaa cttcttctta accttagaaa atttatcatc
    19201 tactttatta ttaatatttc cttccacaga tgttccctcc aatcatattt aatcagctga
    19261 gtttagctta taacctatta aagatatcac aaaactatat ataaatgaaa ttacaatagc
    19321 ttgtaagact ttaaattgca ttattaaagt tgctccataa ctagttgtat taaagaaatt
    19381 aatattacta tctagtataa atgtactaaa taaagccaac atccccatta aaaaagcata
    19441 atatatacta aatactacta taggttttat actatccgta ttatacttaa atcttagctt
    19501 gtatccattt aataataata ttaacataga taatgctccc attgcataaa acattaattt
    19561 agtatctcca aataaagatg aagaactcaa agtaaacatt gatactgttg aatttattat
    19621 ggttactgaa attccatttg caaaagccca catatagcta gctatttgtg gcagtatagt
    19681 aaataaattt aatccatttg agtaactact catatctagt tcatttaagt aactactatc
    19741 agatatagtt agtacaaata gtattattaa tactaaaaca taacctaata taaatgtttt
    19801 tatagaattt gaaaagagag ataaatacat attttccttt tcataacttt ttttataagt
    19861 agttatgtat gtacatataa accctaatac aaatccattt aaaaggacac taaaaaattc
    19921 ataactatat tctaatgcat atccatattc taacatacta tgtgaactag tttttacatt
    19981 agtaaaaata gataaaatag ctaaaactaa tccataaaaa attccaacac ctatagaatt
    20041 tgctaataca gattttgaat ctctacatct ttttctcata aaaattaagt ttgatattaa
    20101 taataccaaa ataggaatta taagtaatat taacaaacct atattggcat ttataaaccc
    20161 agaacccatc atggtagaca tagatacact aatttgacct aagttgagag ctaggattat
    20221 atgtgctgga tttattaaat aactaatatc attaaattga atagttataa tagctttaaa
    20281 tatcaatgca ataataaata atataaatat ggttgtaaat gttgttaaaa atactcctct
    20341 tttatttgca tgatatgaaa gctcttttat tttagatttt aagtctagtt ttgtttcaag
    20401 tggtcttaat gatgttattt cataaagttc atttccacaa aatttacagt aattatcctt
    20461 agtattattt tctttattgc aactagaaca aattttttct tctttatttt taagagtgat
    20521 atggctattt tttatttttc tagttggttc acctgtcgtt ttggaataat tattatgtga
    20581 ataattcatg ttatttattt tcttttcacc catttttccc ctccaagttt tactattgta
    20641 aattattaaa tacaactata tcatataaaa cttaattata ccttaattgg atatatttat
    20701 ctattttatt tgtttattgc tgttgttttt taacaggtct tgatatttaa atatcttgtt
    20761 aatgctaact ttattattta cattttatta tgcttaattt aaatttattt tattctttat
    20821 tttcagtagc aaactatctt taaatcttaa ttataataaa gtattaacca ataaagttat
    20881 tcaacattag ctaataaatt ttatccatta caattttaga ttgttacaca caatatattt
    20941 tgatataatc aaattataaa caaagtattc caagtatcta aagacataaa ggaatactta
    21001 tttttatgct ttatttttaa tggaggctta tatgaactta gaaaaaatta aaaaattttg
    21061 gggatttcct ctagataaaa aacataaaga aaatgttgaa aaagaacttt gcaattatct
    21121 ttaccatact ggtaaaatca tttgcataat aatagtaata tctcaaataa ttatgatgat
    21181 atcaatctcc gctagagagg gaggtccttt tgccacacca agacgccaat ggtattttta
    21241 tatgtatgta acactatttt gtgtatcttc tgtctttatg tttgtatttt cttatctttg
    21301 gaataaaaaa aaggattgtt gtacttttta tataaattta tggataattt atgtaacttt
    21361 tttctgccta tggggttgtg taattactct acttgaccag ttaggtggaa atgacctaag
    21421 tgtatttagt tacattatgc tagcagctgc agctttctca gtattaaaat taggagaatc
    21481 agtaatagtt tttggaggat gttttttgtt tcttaatttt ttcttacctg gtattcaaac
    21541 tataccaggc aatgagttta gtaatatcgt caacacattt tttataacat cccttgccgt
    21601 ttttgtttct acaatgctat atagaagtcg tgtatttact tactatgaca aacttttaat
    21661 tagtcaacaa tatgaagaaa tatatcatat aaataattta cttaacgaca aagtcatgac
    21721 agatgacctt actaaaatga ataatcgtag ataccttgaa gatattctac aaaaaaaatt
    21781 aatcagccat gcaaatcaag aacctataac tggaatgatg atagatatag attatttcaa
    21841 acaatttaat gacaattatg gacatcaaaa gggagactat tgtttacaaa atattgccaa
    21901 agtaattttg gattttacaa gttctgaaaa tgaacatgct atacgatatg gtggtgagga
    21961 attttttata tgtattttta actgtgatga ggaagatgct aaacttaaat cagaatgttt
    22021 acgaaaaatt atagagacct gtaattttga gcgtgacgat atccctttaa aatgtgtaac
    22081 tgttagtatc ggagtctaca caaataaaat tgaaaggaca ataacgctaa atgagtttat
    22141 aagtaattgt gataaggctc tatatactgc aaaagaacag ggacgtaatt gtgttgcatt
    22201 ttattaaaaa gttacatact attacataaa cctataagta ttatattgca aaataaaagt
    22261 aaccaaatta tatatttata gtacaataaa aataacttgc atatctctaa aatagataaa
    22321 tatgcaagtc attttagtgt ttacacttaa aattatataa gttttattat atataattat
    22381 taaaattcag catttttagg agttcttggg aaaggtataa cgtctcttat gttagacata
    22441 cctgtcatat acataattat tctttcaaat ccaagaccaa atccagagtg ggtagcagta
    22501 ccaaattttc taagttctaa ataccaccaa taatcttctt ctcttaatcc catttcattg
    22561 attctatcta gtaatacatc ttctctttct tctctttgag aacctcctac gatttctcca
    22621 actccaggaa ctaataagtc cattgctcta acagttttac catcttcatt cattctcata
    22681 tagaaagctt ttatatcttt tggataatct gtaacaaaca ctggacaatt atatatttgc
    22741 tccgttatat atctttcatg ttcagtttga aggtcacatc cccactcaac aggatactca
    22801 aagtcatgac cagattcaat taataattcc actgctttag tgtatgttat tctaacaaac
    22861 tcagagttta ctatcttatt taatctttct aacaatcctt tatctataaa actattaaaa
    22921 aattccattt cttcaggagc attttctaaa acatagctta taacatattt tatcatatct
    22981 tctgcaagtt ccatgttatc ttcaaggtct gcaaaaacta tttctggctc tatcatccaa
    23041 aattctgaag catgtcttcc tgtataagag ttttcagctc taaatgtagg tccaaaagta
    23101 taaacatttc taaaagaaag tgccattatt tcggcattta attgaccact aactgttaaa
    23161 ttagcctctt ttccaaagaa atcttgagta tagtctattt tgccatcttc tgtttttgga
    23221 atatcattta aatccatagt tgttattcta aacatttctc ctgcaccttc acagtcacta
    23281 cctgttatga ttggagaatg tgcatataca aagtttctct cttggaaaaa tttgtgtatt
    23341 gcatatgcag ctattgatct tactctaaat acagctgaaa aagtattact tcttggtctt
    23401 aaatgtgcta tcgttcttaa atattcaagt gtatgtctct tcttttgtag cggatatgaa
    23461 ctatctgatt cgccttctac aactatgctt tttgcatgta tttctactgg ctgtttagct
    23521 ccttctgtag atactaattc tccctctaca agtatagatg agcttattgc taattttcct
    23581 acctctttaa aattttctac tttctcatca aatactatct gcatattttt aaagaagctt
    23641 ccatcattta gctctataaa tccaaaattt ttagatactc tagacgttct tatccatcca
    23701 gctactttta cagtcttccc tatgtactgt tctttttctc tatacaattc ttttactgtt
    23761 ataaattcct tttgcatttt aaatcctcct agtataattg ttattaattt cttttaaata
    23821 tagtttcaat aaaaaaagcc cttcatccct atataaaggg acgaaagact attactctcg
    23881 cggtaccacc ctagttgtca taatgacctc ttagttaagc aatgcttaaa gcctataacg
    23941 gtgccttccg tctaagtcta cttactaaat aagctttcga ttagaaactc caagatgttc
    24001 ttcaatataa acctcgtatt gagctttcac catcctcaac tcgctagaac tacattttat
    24061 atctactctt ctcttcatag tatttatctt ttattatttc tgctaattaa caaaattata
    24121 ccaatttata aatttttagt caataaattt atataattga tactgattgc tatttaaaat
    24181 ataataactc taccttgcaa aatgacaata aacacttatc tttactactt cagtttcaaa
    24241 acttttataa tttacttcca ttatcaaccc agtcttttgc ttctgtaaca cccttaattg
    24301 aaggtattga aactttacta tgaggtttta atttttcaac atcagcccat gctgctgtcg
    24361 actcatttcc aactaaaagt gaacttttaa gtcttttatt atctttacct tttaatgctc
    24421 ttgctttttt attttccaaa actatcacct ctattattta ttttgtaata gttttagcaa
    24481 tttttcaaat aaaattcata agttaaaatt ataatgattt ttaagtcaat aacatatctt
    24541 ttttatacta aatctataat tatttttata taaaaatagt tattttatat atacaacata
    24601 taaaactatt ttttaatttt tatatttact ctaattcaat tttttgttat ctgaactatt
    24661 aattaactat aaatttatgc agattattat ctatagagta tttgctattt gttgaattgt
    24721 attttttaag tttaaaatcc catatccttg tgatacattt ggatatgtat atatctcaag
    24781 ctttgtagct cccaatatca aatatgtctt taaaacttgt gtaaacaatg ataatcttgg
    24841 tatattattc tgtttttcca aatattccat tagtaaagct aaaacacctg tgactataga
    24901 actgctcaca ccggttcctg ttcctgtatt atacgttcca tttttatatg tagatattat
    24961 atctacacct ggagctacta tatctggctt aattcctctt cctcggattg gaccttttga
    25021 agaacctatc cacatgctgt cagtcttatt attgaacgta ccaactgtaa ttacatcatc
    25081 actagcagca tacatagtta ttgtagctac tgaatcaggg tctaaaaacc ttgtatctgg
    25141 agctatcaaa tttttatttg gcaagtatat atcatattct ccacctatta taagttctga
    25201 agtcaatctt aaagtccata ctccaggttt tatatctctt aatcttattt ccaaattttc
    25261 ttttccagaa gtaatatatg gataaataaa tctcattgca taagtagtat tttctaagtt
    25321 aaattttcct ctatatatat aaaagtctgg agcatacctt atgtcatgac taacttcgcc
    25381 tgaaggagat attatctgtg cacctacttt atctggacca ttagtattta gtgttatatc
    25441 cagtgcatag tcatctccat cctgtattat cacatcttga acttcaccta cactactaaa
    25501 tcttccagaa tagtgaatat ctgtattacc ttgatttcct gcgccactca ctactactac
    25561 accagcacta ctcaatatat taaaagtatc tagtattgaa gtagttgcta cagctcctga
    25621 cttaactcca atagtcagat ttattattag tggtctattt tctgttcttg ctatattagt
    25681 aacatacgtt atagctgcta aaaaatctga aacactgtaa tttatccttc cagcataata
    25741 agtacctata taagatttta atttaactac tattaagtct gattctgtag taatacctct
    25801 atacttcgaa tttacccttc catttccagc taatatacca gaaactaaag ttccagtacc
    25861 tatattatct tgactcaaac ttccatcatt tctatttatg gctatattta actcacttct
    25921 tgtaaattca cttccaaaaa taaaaccttc tggaggtgga tttgtatttg cttcttggtc
    25981 ccataaatat aaaactttac tggttccgtc atcatttata aaatcaggat gcaaataatc
    26041 tataccagaa tctataactg ctaataaaat tcctcttccg gttatatcat tatatggatt
    26101 ttcatagata tactcagttc cagcagccgt tgtaatggtt tctccattat ctacattatt
    26161 ggttatttca attaaactgc tcattggtgc agattcttcc caccaagcaa cctgtattat
    26221 attatttaga atagtttcat caaaatctat aggtacatat attactgcaa gttgactatt
    26281 taaaaccata tacctattta ttccattttc ttgtagtgca gactctatat caccttgata
    26341 aattatacaa taagattttt ccataaatcc ctcctatctt aaaacatcaa acattcctct
    26401 aacatccaaa agcccatatc cagaatttgt atttggatat actgtatttg aatcctttct
    26461 agctccagct tgcataaacg tttttatttt ttgaacatag gcttgatttg gatatctacc
    26521 atcaacaaaa gtatattgaa aatacattgc tgcagcacca gctgcatgtg cacttgctgc
    26581 tgctgtacca gttatagttg cataggtatt cccaggataa gcagctatta tattaacacc
    26641 aggtgctact atatctggtt ttaatctgtt ttcaatagtt ggtcctcttg atgaactttg
    26701 ccataaacta ctgttaatag tattgtaagc tcctatagtt attaaatcat cttgaatagc
    26761 aggataattt atagtataaa aagggtctac ctctctaaat ctagttccac tttttaaaag
    26821 ttttctattt ggaaggtata agttatatct ccctgttata atataaaccc ctactaatcg
    26881 aatcttccat acacctcttt ttgcattctt taaagtaaca ttagtaaatt gctgtccaga
    26941 aaaagtagtt ggatatatat acgttataga atattcagtc ccttctaagt caaataaacc
    27001 tgttacttta ttataatttg aaactccaac agttttactt tcttcaccag taggagatac
    27061 tattattacg tcagctttat ctggcctact taaccaaagt tctaatgata attcttcctc
    27121 ttcttcattt aactctaact caacttcaac agagcctcca acatgtgata ttattccaga
    27181 tgtatgtgtt tgtgtattgc cctcattccc tgctcctgct gttacacata aacctctagt
    27241 aaaaaatgct ttttcactat taaccctatt ggttaaacca gctaaactac tggtacctaa
    27301 agatacattt ataactaacg gtcttcccag ttcaaaagct ttattatatg catattgaga
    27361 tgcagcaaat aacattgcac tattataaaa accttctatc ttaccaagtt ttattactat
    27421 taattcagaa tcttctgcta cccctgcata ctcactattc acatttccaa gccctgcaca
    27481 tataccactc aacatagttc cttgaccaac ttcgtcctga gataaactag ggtcatttgc
    27541 tgcaatagct cgatttatat cctctcgtgt atattctgta ccaatataaa aaccatctgg
    27601 tggatttcct tcttttgttt ggtcccatag atatactatt tttgaggtac catctggata
    27661 aataaaatct cggtgtagat agtctattcc tgtatctgca attgatatta gagtacctct
    27721 tcctgttata ttaatattag gattattttt aaagaaattt acgcctattt cttctgttgc
    27781 aactactcct ccaaaagtac ctctattaat ttctccaagc aatgtcatct ttgtagttgg
    27841 tatagttctt atgattgaag gaagttgtaa aacttgttgt atgctattat agtctgtatt
    27901 atctatagat agtacaccta atgaatcact tatttttaaa aaattttgac tctgacctaa
    27961 cccagctaat tcttcttcaa attcaggagt atggactaca tctattcctg atagtattcc
    28021 tgactgtcta cttatatttt tacttatata cttttccttc tctaacccat tatgtatttg
    28081 tacattataa ccatctatgg gtatataatt atttgcctca tccataaatg acttattaat
    28141 acttctatat ccaatatcct ctgtttctaa atcttgattt atgctagata gagtgtacaa
    28201 atctacatct gataaattta aaaaaccaaa tcctgatgaa ttatttgggt aagattgatt
    28261 gcttaatctt cttgcaactt ttaaaagtaa tgccctcaat ttctgtgaat acaaaaataa
    28321 atcatttcca tttacaattc cccattccat aaacaatgag caaacccctg taacatgagg
    28381 agttgccata ctagttccag ttaatgcccc acttgtacct cctggtaaaa aagatactat
    28441 atcttcacct ggagctaata ggtcaggttt aaagacgcct aattgtgtat ctccttctcc
    28501 agaaaatata gatactatgt ctgttcttga attgaaactg cctactgtta taactctact
    28561 tgctgttcca ggaactgtta cagtaagctc ttgagtagga atcaaaaatc tagtatttct
    28621 atttagtcct tcagaagttg gtaaatatat atttacatta cctgttacta tatttattgg
    28681 ttcaaatatg attctccata atcctggagt tatttgtgta tttgaactta attgcaaagt
    28741 aactcttctt gtaagagaat atggtgctat aggataaaaa taaccagtta ttctagtttc
    28801 acccaaggta ttccttattt cacctgaagt tagagatatt gtttgtgttt gattatttga
    28861 tgggtttact aaatgcacac taaaatcatc aacaaaatct ggccatatat ttacatttag
    28921 tatacgctct ccttctccaa ctataaactc aacttcttct gtaatattat ttctaagtct
    28981 aatcctttta tgaccacctt tatctgcatt atttccagcc gctacaacta tattattttt
    29041 ccaaaataaa cacatatcat ctatatattg ttcaaataaa gacgttcctc tatgtgaacc
    29101 ttcattgctt ccataactta tatttaatgt aacaggcatt tttaactcca atgctctatc
    29161 taaaataaat ttaattgccc tcataaactc tgtacttctt gaaaaaatat cggtttgtat
    29221 atttccaact ctcaccacta ttattctggc atcacttgca atagttgcac aaatacctgc
    29281 aacatgtgtc ccatgtaaac tagttgttga aataggtata tacatacttc catctattgc
    29341 attatttata tcttcattag tgtataatgt tccttctcta aaaccttctg gtggatttcc
    29401 ctgaatagac tggtcccaat aatataatat cttagaccta ccattactat ctctaaacac
    29461 aggtaaagta taatctattc cagaatcaat tataccaatt atagttcctt tcccagttaa
    29521 accagttcta tttttaaacc ctgttattcc tgtacttgaa aaactttgta catcctgagt
    29581 ttgtaataca aaaggctttt ctataaattc aatttctgga taagttaaaa gtgtatttac
    29641 atcttcttca ttacttgatg ttattattgc atatgatgaa ttaagtatct ctactgatac
    29701 acctagttct tcttctaatc tcaatatatc tccattgtat tttacaatta attcataatt
    29761 tattataatc acctcgactt ctaaaattat tattaatgta tatgttctat gtaagtctaa
    29821 aatgtatcag actatataat ttttaagttt caattatata atttataata gagatactaa
    29881 aaaaataaat aatgatggta caattttgaa taaatcatac catcatttat atttacttta
    29941 tcttgaaacc attaaatttt tcagtgcttt ctcaagacct tttacagtca gtggaaacat
    30001 attaaattca ctttgtatca ttctttgagt ttgataccaa taattaattg aatcccatcc
    30061 atctctcagt tctggattca tccatacagt tttactgtac tttctcctaa accttttaag
    30121 ccactctata ccaggtgtgt aattatatgg accacgatag ttaccaccaa tatgaagtaa
    30181 ctcagaaggt gacatagaag catctccaac aattataact ttatagtttt tatctatatt
    30241 ttttaatacc cattcagtat taatagattt acttaaccaa cattcaggtg ttgtaaataa
    30301 tctatcataa atacagttat gaaaatagta tatctttaca tctttaaaat gatttgattt
    30361 acttacagac tgaaataatg tattgcataa agtgctatac cctctcattg aaccacctga
    30421 atccataagt aataaaagtt ttactgtatt ttttcttggt ttctcaaaaa acagttttaa
    30481 ataacctgca ttattacaag tctcttcaat agtcttatcc aaatcaagct ctgtctttgg
    30541 agcatctatt ctagtagaaa attgacgcag tctctttagt gcaacttgaa attgacgcat
    30601 atttaaaact tcatcatctc taaaatctct gtattttctt tctcctgcaa cttcaagaac
    30661 agctctacca taagtagaat ttccacctat acgtatacca gtttgccctt ttcctgaatg
    30721 tcctaactca gaagttcccc ctgttcctat ccaataatta ccaccattat gaggagaatc
    30781 ttgctctttt attcgttcct ctaatttggc tcttaatttg tcaagattta tatttggatt
    30841 ttgttctaac ctttcaaact cttctaaatc tatatctggt tttttaagcc aattcataat
    30901 ctcttctgga atttcctctt gatgttctat acctttaaag tactctaaaa atactgtgtc
    30961 aaacttatca aaatcacttt cactttttat tagaatcatc ctacaaagat agtaaaaatt
    31021 tgaaaagttt gaatagcaca gtcctttatc caatgcttca attaaagtca tccattcatt
    31081 taaagataca tttactcctc ttgacctcaa taaataaaaa aattcaataa acataattat
    31141 ccagctccta ttaactataa gtgtctttgc atagattcaa tatcctcatt atttttaagt
    31201 agtattcctg caaaaggaac ttctttttct attttttcaa taggtattcc actcaatgtt
    31261 aatgcttgta tccagtcaat taattctgat gtactcggct ttttttgtat atcctttaaa
    31321 cttctaatcc aataaaaagt agtcattact tgttccaata ggtgttcttc tactttatca
    31381 aaatgcactt tgactatttc ctccatcata tctctatcag gaaattctat ataatgaaat
    31441 atacatcttc ttaaaaaagc atctggaagt tctttttccg catttgaagt tataataact
    31501 attggtcttt gtttcgctct tacagtttct tttgtctcat ttatgtaaaa ttccatttta
    31561 tccaattccc aaagcaagtc atttggaaat tctaaatccg ccttatctat ttcatctatt
    31621 aaaagaatta cttgttgatt tgaactaaaa gcctctccta atttaccata ttttatgtac
    31681 ttgctaatat catctacacc ttctcctcca aattggctat cataaagtct ttgaacagta
    31741 tcatatacat aaagcccctc ttgtgctttt gtagttgatt ttatattcca aattactaaa
    31801 ttttttttta attcatttga aattgcttgt gctagcatag ttttacctgt tccgggttct
    31861 cccttaatca aaagtggttt ttcaagtgca atagcaacat taactgatgc catcaattct
    31921 ggtgatacaa cataatcact acttcctcta aaattagata tctctttttt tatcataaat
    31981 tctactcctt ttaaacagta ttttctaaat gtatatatac tttattacta tttttgatat
    32041 aaaacaataa tgtaatacat ttgtatagta ttattctaaa attctatatc taaataaaat
    32101 t
//
LOCUS       DAMJVM010000236        22544 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.guided.054, whole genome shotgun sequence.
ACCESSION   DAMJVM010000236 DAMJVM010000000
VERSION     DAMJVM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22544
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.guided.054"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            278..1210
                     /gene="blaCDD"
                     /locus_tag="PC523_003563"
     CDS             278..1210
                     /gene="blaCDD"
                     /locus_tag="PC523_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HDF2477013.1"
                     /translation="MKRKKNFIWTTILLVGIVMVMYYAGKKHNDINQKTDKNYIKSDL
                     NKSEINNDNNKKERVNIVNYSDCFEGISGGAIFYNSRNQEYNIYNKDLIETRASPCST
                     FKIISTLIGLEKGVITSKKSVIGYDGTNYPNKNWNKNLSLEEAFKESCVWYYKKLINK
                     VDAKSVQSILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMVKIF
                     EGDTNFKKEHINILKDIMKIDVSDKNINVYGKTGTGFNGKNKRVDAWFVGMLESKDDT
                     YYFAIKSDDSNKEIAGPKVKEIAINIIKKYYSVM"
     gene            1559..2242
                     /locus_tag="PC523_003564"
     CDS             1559..2242
                     /locus_tag="PC523_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2477014.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHMLGGVDRPTSGKIYVEDTEISSLNEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     TILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            2239..4899
                     /locus_tag="PC523_003565"
     CDS             2239..4899
                     /locus_tag="PC523_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2477015.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDDTYGKDLSLIENFSSVDDVMVKGQWYVGKINNDKRDYI
                     VYRNATSEYWNHMPEKDSIIEGRRPSNTNEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPVAPKSKGESFKKISKTTLTIVGKVDVATSSVVPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SNISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNKLIVYINSVNYHTDAPEVVFTYGIPAFLPAVLL
                     TIVTVWISTLIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTGFLHIIANQEGAEAVYGSDNYKDQRDIEVYLENSEPVE
                     SDFIEKLDNTEGVKNSLYYQKMSATTWLTEKDASKELSKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDESFAEYCRQISVDPKLFYDTKNPTSIVYNKEEDVNRSTRRNKVY
                     IDYLNLSIGDNIKLNEKIYDEDKGDYTYNMKVGAITSTLPKIIDTSSRYTLMQIVPIS
                     MVQEISKNYAEVGRSRSNRLIGLFKVDNREDIPRVRGKLESICNKDYGSGDYSIEDVV
                     ENEKQNGSGRKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPD
                     GIKKMLVLEGLFLGITPLLLSIPVQIGIVYTFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYNQLKNENIVESIKSETI"
     gene            complement(5076..6257)
                     /locus_tag="PC523_003566"
     CDS             complement(5076..6257)
                     /locus_tag="PC523_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2477016.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKVL
                     DASYEYNPKSTESILKSLKNSSDEDFKTGNEILSKHGYSKGTFWSKNFFYFLVVSFGI
                     ISTMAIVVYIIKYRTNKQKLSRINMLTRYLEEVNLGKESVLLRYEDEFSYLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TKQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     KGSPSIEFEIDINWTLEALLNILKNCSEHIEAGGHIIAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(6257..6919)
                     /locus_tag="PC523_003567"
     CDS             complement(6257..6919)
                     /locus_tag="PC523_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2477017.1"
                     /translation="MAKILLLEDDDTITFGIKTSLENKNHIVCCYNTIAEAKKNFDKT
                     TDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRAHSNTNNTITCGNISLNKVEVKAYLDSVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            complement(7035..7673)
                     /locus_tag="PC523_003568"
     CDS             complement(7035..7673)
                     /locus_tag="PC523_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2477018.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFSLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSKKSDTEMLFKEKFFSFNKFMFDEIQEMFEIGKNDGSIRYDI
                     EISTLMYSSIFTLTGFFNLLSVTRKSYLNNFNIDEEKFIETTLAILIDSIKA"
     gene            7853..8458
                     /locus_tag="PC523_003569"
     CDS             7853..8458
                     /locus_tag="PC523_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2477019.1"
                     /translation="MKIKENVLRLDSTRGSNCFIVFDKEITLIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHMSNIKLLKEKTGAKVWAHTEDIPFIIGEKDRPGFKK
                     FIGKTISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVSYPNPWNWDSKKMMKSIRELDNLEYEWICMSHGTPCIK"
     gene            8559..9281
                     /locus_tag="PC523_003570"
     CDS             8559..9281
                     /locus_tag="PC523_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2477020.1"
                     /translation="MIRTLILYYLNIKPTHGYEIQKFLQVSGADRWTKIQSGSIYYAL
                     AKLEKDGGVRVLKEEKTGARIRKIYEITQSGKLELREEIQKELQMPIVPTGSNKFLLH
                     NILDVLPKDTLQKNLEKHIKYLIEQKKYWENWKEIKKIDKKSLATEKIAFDMTIDNLN
                     YQILWHEEILNDIDKYISVGCETQNIIKSIDFSNIEEDFLFTSDTTNELLEVQRLRDE
                     IINNPDKAIENIDKIILKLQNK"
     gene            9383..10141
                     /locus_tag="PC523_003571"
     CDS             9383..10141
                     /locus_tag="PC523_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2477021.1"
                     /translation="MSNILLEIKNLSKKYNNKEVVSIESFKLLEGDILGFIGANGAGK
                     STSINMLTTIVSPDSGSIYYKGKDIKNQQNIFKSSLGVVPQELAIYEDLSAYDNVKFF
                     CSLYGFKGNELKERTKDALEFVGLWESHKEFPSKFSGGMKRRLNIACAIAHSPKILIM
                     DEPTVGIDPQSRNKILEAIKCINTNGTTVIYTSHYMEEIETLCNRIALIDKGIIIEDL
                     DKSLYKNKYLEFGCKTLEDIFLYLTGTDLRDLEE"
     gene            10146..10886
                     /locus_tag="PC523_003572"
     CDS             10146..10886
                     /locus_tag="PC523_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2477022.1"
                     /translation="MRCFLPLFKLGIKRRSKDFFILFYNIVFPVIIIMLLGYLTSKSY
                     GEEFTSYNYYTIVTIPFCVLMGIISVSYAGQDEKISNTSYRYMISPISIVDLVISKLF
                     SCTAVLSICNLVTLVVLKCIFRMSFGGKFLAVLFFLMCESIVVVSMGLFLGLVCKNLD
                     MLRNIINIPIVIFGFLGGVFFPVSSLNPVISLIINISPLTWINRGIVACIYDNNLQLL
                     FNISCIFVVVSIFMTILTIKFFKKGVFI"
     gene            10886..11938
                     /locus_tag="PC523_003573"
     CDS             10886..11938
                     /locus_tag="PC523_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2477023.1"
                     /translation="MNVINVFNINLKRNINKRIVFLVTLLFPIIIVILGVLANYISKP
                     FFNIGILDEYKNERVLKESEKRTYENTIMALENTQGINTKIADFKTIKTDIITGKYSA
                     VIYFNKNGFKLYSIKNKNTNDTLKYLIETYKKDPVPVDISKLQENTLGVLQRIIAFIV
                     LFLMITSTVTASMIIKDKNSGTFTRVLYSPQNVRGYVLGNMLYNFTITYFQYIVSISI
                     IKLFNIDIGMDYIDLILMGIWVVALATSFGTFVSSLFNREMHANLFSTCISLVLSLIG
                     GSFISVEKMPEMLQKISIISPVRWFISFVTYIEERDGSFFNMNYICILTSIIIVLALL
                     AMKFTQRSKYSSNSNY"
     gene            12340..12906
                     /locus_tag="PC523_003574"
     CDS             12340..12906
                     /locus_tag="PC523_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2477024.1"
                     /translation="MNNLFYDKLNFDDKLIIKNNGVIRNNDLYFLKYKNDTAGILIYN
                     IINDKFSFKQNKNYKGMNPFPITNIFKNLDSDKWDEFIRIWIEERVIQECRPEVPFIL
                     KEFKLGYYDVWDICRSNLAMSVEDFFWITKDNTLRYEDFNIRYCTLNNITPIYKPPFP
                     IVIYPNYFEREKIKSGETWKYELKNTNS"
     gene            13402..15615
                     /locus_tag="PC523_003575"
     CDS             13402..15615
                     /locus_tag="PC523_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF2477025.1"
                     /translation="MNQSYHNIKNELLSKQLAYIDRLTGYLVDRDNYLSGYVKEKLKI
                     AKEASIKDNTFLVNVKALEANQPKYETDVFFSIDSTWISSSIKSDFIEKTLELKENSI
                     QLLYSTQLGYSVKCSDIIPISKNEFTWGTKRRKSLYIVEAELNMKTITVTDKEYQNGK
                     EVSVKNLEETQLAMNILNKWLIAFHNYVTSNSYIHKELLDRYNEMFIDYKEKEYKNIL
                     NDVRINPNIKLREHQLKAASRIITSDRNTLLCHSVGSGKTYTMITAAQELNRISKIDS
                     KKGNKNLFVIPNSLCESGQFAKEYFNLYPQANILATTSKDFSKSNRRKIIAKIVTCNW
                     DSIIIPHSVLGLIPLEADTELKLLDLYLKELNDTLKYYKKECPDLSTKRLETMKENYL
                     NRIKELNDMHKDDALLSFEKLGITNLFIDEAHNFKNLYFNTTMQVPGISTLKTKKTQD
                     LYNKLRYFKSIHGNKGVTFATATPISNSICEMYTLLTYLNKDTLKYYKVDTFNAWASK
                     FGSIISSMEVDPTGQGFRFQQRFAKFCNVPELVNIFRQIADVVNICDINDLDIPQIKG
                     GKPIIEIIKPTNQMKEYIDSLVKRAEKIHEGPYDPIIDNMLNVTTDGRLMAVSPALVG
                     IDGILPKIIKVGENIASLYHEDKISTHLVFCDLGTPNGNSHNVYNDIKNELLKNNVLE
                     KDIAYIHDANTPEKRKKLIDDFNNGIIRILIGSTSKITSSCRCSLETFINFTKRR"
     gene            15788..16018
                     /locus_tag="PC523_003576"
     CDS             15788..16018
                     /locus_tag="PC523_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2477026.1"
                     /translation="MSYAETKACACSDDSILKLCEINREIQIIEINKKAFLNQNLLTL
                     NDIKSTENSIDALKISISKLEEDIKTYFSNFF"
     gene            16134..16490
                     /locus_tag="PC523_003577"
     CDS             16134..16490
                     /locus_tag="PC523_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HDF2477027.1"
                     /translation="MNIIYEKEFTFDNKVREFISLGNNYKFEFSTSKSPKTLIKYFMD
                     YNKISSNKLESFKEKYSFKKNELLSLQEILKNKFTEDDKLESLKLSKANLENELKLGN
                     SIIKKSNSNHNMVEKK"
     gene            complement(16692..16853)
                     /locus_tag="PC523_003578"
     CDS             complement(16692..16853)
                     /locus_tag="PC523_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2477028.1"
                     /translation="MIDNKYIFRGKNIKNYIKFKDITGANKQDSLIYLKALSIEREEY
                     LINRLKSFF"
     gene            complement(17435..18247)
                     /locus_tag="PC523_003579"
     CDS             complement(17435..18247)
                     /locus_tag="PC523_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HDF2477029.1"
                     /translation="MNYSDMHEISSGKSQKIALIDSGISKFQKVYKNINLTNSNNDYD
                     TNGHGTMMYSIIKGYNKNNQTIEGISPDSRIILIKVMNDDESINPNVIEDAIDYALNE
                     KVDIISLSLGSTKTDINIKQKIDKANLKNITVVSSAGDYKQGFLLFPASLNNVISVGS
                     IDDRRDISTLTNAPQKTLINAPGEKIKVIDNNNNVFYGKGTSEATAIITGYIALMRDT
                     NISSTQILTNEKIMNLLSQINKNEKTYLGALKEIRKKVSVSTGAFFSFDLSY"
     gene            complement(18395..19081)
                     /locus_tag="PC523_003580"
     CDS             complement(18395..19081)
                     /locus_tag="PC523_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2477030.1"
                     /translation="MKYFKKVIALGLSIALLIPVISINALEKKDTNVNNQIINVIESN
                     VSYTKEGIEVSSDLKNDLKEINLEDIKEQMIEQGIDSKFINNFNSDVIYSLVKENIDD
                     INEGVKNKTLKITPEEDIINNTDNSFYVQGGSTYTQKFSWGVRQNKSTAAANRWVYDL
                     NKSSQGFVATGAVSVLLGSMGAPIGFVSGLSAAYMGGLANKVSYVNSRNNRGITVDIN
                     WWLTYAVKNQ"
     gene            complement(19111..19533)
                     /locus_tag="PC523_003581"
     CDS             complement(19111..19533)
                     /locus_tag="PC523_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2477031.1"
                     /translation="MYLKKVIVAGLCTLTCLLPLNVQAFEDTSLNTNDYNQTNDNDLF
                     MIYIKGAGSNLSIRSGKAHISCYVDGKIGSLTSITARLQQKKSGKWVTISTWSSSGKT
                     SSTLSREYRVSKGYSYRILTTIKAGNESRNITSNVTNY"
     gene            complement(19608..20066)
                     /locus_tag="PC523_003582"
     CDS             complement(19608..20066)
                     /locus_tag="PC523_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HDF2477032.1"
                     /translation="MSVDTLRNQFIKIVIEIYEDLTEFIFSKDIQTDKNLDFILKEPK
                     YIPNGFKEVEKENTLQGIFIRYENKNGSEIRYRSNQIDSNTVILDTENAKVTDIMVND
                     YEAKYIVKDNSITIFWNDEENVYTIYFDYDNKNDLNKYKDNLIDIAENIK"
     gene            20259..20909
                     /locus_tag="PC523_003583"
     CDS             20259..20909
                     /locus_tag="PC523_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2477033.1"
                     /translation="MEVIRNMTNVSSLEKEDEIVEILSSKENIRVAIEIERGLKLCKT
                     QMIKKVLEEIEKRMDKKFEDKYKLPYYSYKENNYALVNNYYNKKSSTYPAINYFIKSL
                     DKEDVDLLLRIEIDHHIFVGFCTLYKEKPSGKILSDDEIKELINDDGSRTNGWWICWE
                     YIYNNTMECPNFKNFNDAYFDLFDDNKFDEFMDLCEKRILSILGKLKDKQCINTFI"
     gene            21060..22091
                     /locus_tag="PC523_003584"
     CDS             21060..22091
                     /locus_tag="PC523_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2477034.1"
                     /translation="MKIEMGESLLYSWLRHVKECQIVQTNWKVSSKWDLKNADKILEI
                     MEKTKQYFEQKYGYPIYKNNSLEQLIMQAEVDVLGISIDNGRNHIYAIDVAFHEAGLN
                     YGSKNETVTRVIKKCIRTAMCIYGYMDLDEAEIIFASPKINPAIITELEPKMAEVNDL
                     FKSLGLRFKTRLIANEEFNGKILEPILIASDGISDTSELFLRSYQMYSMFSYSRDVVK
                     QQRIKKSNSLKDAVELNLSDETLKELKIGKLVQVTMKHLFEEEKISPEEIDKMTQSSY
                     SKQVFNENKPVLKEVPKDGNINELKKDEKNYNRYYAYTISIYGKQYLLVSQWIEDLHR
                     SYYESWVIK"
BASE COUNT         8672 a         2675 c         3222 g         7975 t
ORIGIN      
        1 attgtaatat ttaaatatta caatagtaag ttttaaaagt agaatcaaat actatatcac
       61 aaattaaaag gaggacaaaa tgctattaat agttacctaa ttattttcgt aataggttta
      121 atcgacatag ttgtgctagg tgaagaaaat ataagatata tagttattcc tttagtctta
      181 ttttttataa gtagcaccta gattgaagta tattacaaat aatgtctact attttaaaag
      241 taggttataa atttaaatat gggagatgat ataaattatg aaaagaaaaa agaattttat
      301 ttggacaact atcttgttgg ttggtatagt tatggtaatg tattatgcag gtaaaaaaca
      361 taacgatata aatcaaaaaa ctgataagaa ttacataaaa tctgatttaa ataaatctga
      421 aattaataat gataacaata aaaaagaaag agttaatata gttaactata gtgattgttt
      481 tgaaggtatt agtggaggag ctatttttta taatagcaga aatcaagaat ataatatata
      541 taacaaagat ttaattgaaa caagagcttc accttgttct acttttaaaa taatttcaac
      601 attaattggt ttagaaaaag gggtaataac gtcaaaaaaa tcagttattg ggtatgatgg
      661 tacaaattat ccaaataaaa attggaataa aaatcttagt ttagaagagg catttaagga
      721 gtcttgtgtt tggtactaca aaaaattgat aaataaggtt gatgctaaat ctgtccaaag
      781 tattttagat gatttaaagt atgggaactg tgatattagt gaatgggaag gagatttaaa
      841 aaatggaaag gggcatttaa atggattttg gttagaatct tcattacaaa tatctccaaa
      901 agaacaagtg caaacaatgg tgaaaatatt tgagggagat acaaacttta aaaaagaaca
      961 tattaatatt ttaaaagata ttatgaaaat agatgtaagt gataaaaata taaatgtata
     1021 tggaaaaact ggaacaggat ttaatggaaa aaataaacgt gtagatgcat ggtttgttgg
     1081 tatgctagag agtaaagatg acacttatta ttttgctatt aaatcagatg attccaataa
     1141 agaaattgca ggaccaaagg taaaggaaat tgcaattaat ataattaaga aatattatag
     1201 tgtaatgtaa taaacttaat tttatttgta aatattggtt acctatagtc tgtaaatatt
     1261 tattaaataa ttttactaaa ttgagtttac atagagcaaa actcctctag tatataaaat
     1321 acttgctatt gattattaaa ttgaatactt atagatatta aagttaatat cttttaaagg
     1381 ctatgcaata tattaaaagt ataaataaaa tatattgcat agtttttgtt ttgtattata
     1441 attttaaata atgttcaaaa actaaacaat atttttaata gtaatcgata tttgtattca
     1501 tgtcactata atgtcatttt aggttgctaa aatttaacca taaaggagat gagtgtatat
     1561 ggaaatatta aaatgtgaaa atctcacaaa aatatatggt tcaaatcaaa ccagagtgac
     1621 agctcttaat aatgtaaatt tatcagttca aaaaggtgat tttgtatcca ttgtaggagc
     1681 atctggttct ggcaaatcta cattgctaca tatgcttggg ggagtagata gacctacatc
     1741 tggaaagatt tatgttgaag atacagaaat atcaagctta aatgaagaag ctcttgcagt
     1801 ttttagacgt agaaaagtag gtcttattta tcaattttat aatcttatac ctactcttga
     1861 tgttagaaaa aatatattat tacccatgtt acttgataaa agaaaggtgg atgaagatag
     1921 attctctgag attgtatcta tattaggatt atctgataga ttgaatcatc ttccaagtca
     1981 actttcaggt gggcaacaac aaagggtatc tatagctaga agtttaatct atagaccaac
     2041 tattttatta gcagatgagc caactggtaa tctcgataga aaaaattcag aagaaattgt
     2101 agatttgtta aacttatcaa ataaacgatt taatcagaca atacttctta ttactcatga
     2161 tgagaaaata gcactagagg caaatcgtat tgtaactatg gaagatggag tgattgtctc
     2221 tgaaaaggtg gtgaagaaat gaatattctt caagaatata ccttagactt tgtacgacgc
     2281 aataaacgta gtagtatagc tataatgtta gccatattac ttaccacaac catgatgtct
     2341 tccttgtgtg gattgttgta taccatgtgg acagatttta taagactttc aattgagaaa
     2401 gagggtaact ggcatggtga attgtttgac gatacttatg gaaaagactt atcgttaatt
     2461 gaaaattttt cttcagttga tgatgtaatg gtaaaaggac aatggtatgt aggcaaaatt
     2521 aataatgaca aaagagatta tatagtttat agaaatgcta catcagagta ttggaatcat
     2581 atgccagaaa aagattctat aatagagggg cgtcgaccaa gtaatactaa tgaaattgca
     2641 ctatcaaaac aatattttga gaataatcca agtgtaaaaa ttggtgataa aattactctc
     2701 ccaataggag atagaatcct aaatggaaag cctcttgaac ctgtagcccc aaaatcgaaa
     2761 ggggaaagtt ttaaaaaaat ttcaaagact acattgacaa tagtaggaaa ggtagatgtt
     2821 gctacttcat cagttgtacc tgcatataca gctcttggat atcttgataa aacaagtata
     2881 aaacctaatg acagtataac tgtgtacttg cgatttaaaa atataagaga cacatataag
     2941 gaacttccaa aacttgctaa atcattggga tggaagacta atgaatatgg aaaatacaat
     3001 cttaaataca atagtaatta tcttttaaaa atgttagtct tttctccaga acagaaagca
     3061 tctatgagca atatatcaaa attttcaact cctattatgt atcttacaat agcaattttt
     3121 acagtagccg tgtttgtaat ggtaatttac aacgcatttt cactatctgc aaatgctaga
     3181 cttacacaat taggaatatt atctagtgtt ggagcatcac caaaacagat taaaaggtct
     3241 gtagtgtttg aaggtttttt attgactatt ataccattac caataggttt atttttaggg
     3301 tggttattgt gtaataagct tatagtctat attaattcag ttaattatca tactgatgct
     3361 ccagaagtag tattcactta tggtattcct gcttttctgc cagcagtttt acttacaata
     3421 gtaacagtct ggatttctac actcatacca gcacgaaaag tatcaaaaat atctcctata
     3481 gaagcaatac gacaaggaga tagtgttaaa attaaaaagt cacataaata ttcattagga
     3541 aagatatttg gaatagaagg agaacttgca tcaaatgcac tacgtgctcg taaaaaatct
     3601 tatcgtactg caacaatatc tttaacatta tcttttttat tactgacagg ttttttgcat
     3661 ataattgcaa atcaagaagg agcagaagct gtttatggaa gtgataatta taaagaccaa
     3721 agagatatag aagtttattt ggaaaatagt gaacctgtag agtctgattt cattgaaaaa
     3781 ttagataata cagaaggtgt aaagaactca ctatattatc aaaagatgtc tgctaccact
     3841 tggctgactg aaaaagatgc ttcaaaagaa ctttcgaaaa atggaggatt taaaagtata
     3901 gtttcatcta aaaagtactc tcctatagag cgtgatggca agtttcgtat aatttcaaat
     3961 ataattgggc ttgatgatga gagtttcgca gaatattgca gacaaatctc tgtagaccca
     4021 aaattatttt atgatacaaa aaatccaact tcaattgtat ataataagga ggaagatgtt
     4081 aatcgtagca cacgtagaaa taaagtatat atagattatt taaatctttc aataggagat
     4141 aacattaaat taaatgaaaa aatatatgat gaagataaag gggattatac ttacaacatg
     4201 aaagtgggag ctattacaag cacacttcct aaaataatag atactagttc aagatataca
     4261 ttaatgcaaa tagtaccaat atctatggtg caagaaatat ctaaaaatta tgctgaagta
     4321 ggtaggtcac gttcaaatag actgatagga ttgtttaaag tagataatcg tgaggatatt
     4381 cctagagtaa gaggaaaatt agagtctatt tgtaataaag attatggttc aggagactat
     4441 agtattgagg atgttgtaga aaatgaaaaa caaaatggta gtggaagaaa gactatgaat
     4501 ttgatagtag gttttattac aggtttattg gctatgattg gattatcaaa tgtacttgct
     4561 acagtgtcag gaaatattag aagtagacga caagagtttg ctatgttgcg ttctgttgga
     4621 ttatctccag atggcataaa aaagatgctt gttttggaag gtttattctt aggaattaca
     4681 ccattactac ttagtattcc tgtgcaaata ggaatagtat atacttttct acgtataaat
     4741 gaaatatatt ttatagagta tttaccattt gcacctatat ctacaataat aggttttact
     4801 atattgatac tttttattgt aatagcatca tatatgacag gatacaatca gttgaagaat
     4861 gaaaatatag tagaatctat aaaaagtgaa acaatataaa taaaagttat ctgaacttat
     4921 ttatggtttc aattcaagaa tacttacgag tatagagata gttaaattgt ttatttaata
     4981 aaatcttaga tataaatagc ggattactaa aataataaat aacataggat atagatatga
     5041 ctggattatg ggtattttac ctataatcca gtattttatg tgtaaaactt aattatgaat
     5101 cttgcaccac cattatggca attttctgca tgtagagtac cattttgacg ttctataata
     5161 gactttgata gagcaagccc tataccaata ctgtccttac tagcgttttt accacgataa
     5221 aatctattaa aaatatatgg gagctcttcc tgagcaaacc ccttaccatt atcttctatg
     5281 ataatctcaa catatagagg gttttgacta taataagcaa tgatatgtcc accagcttct
     5341 atatgctcac tacaattttt aagaatattt aaaagagctt ctaaagtcca attaatatca
     5401 atttcaaatt ctattgaagg agaacctttg atagtaaatg tctgatgttt ttctcttaaa
     5461 gaaccttcta taggctcaat tgcataagta agaagagaat gtacatctat aagtttgctt
     5521 tcaaatatta aagtactcgc atccaatttt gataaggtaa gtaaagatga aactagtctc
     5581 tcaagtcttg atatttgctt tgtaagcctg ttaatataat ctatttcttc atctggacaa
     5641 ttttctgaaa gtaattgtgc cataagtgac atagaagtaa ttggagtctt aagctgatga
     5701 gcaatatctg caaggttatc tgaaagagtt tgtctttctt ttagagcaaa ttcttttgaa
     5761 caacgaagtt ccccaactgt tttatatatc tcatcttcaa gataagaaaa ttcatcttca
     5821 tatcttaaaa gtacagactc ttttcctaaa tttacttctt ctaagtatcg agtaagcatg
     5881 tttattctac tcaatttttg cttatttgtt ctatacttga ttatatatac aactatagcc
     5941 attgttgaaa ttataccaaa cgataccact agaaaataaa aaaagttttt actccaaaat
     6001 gtacctttag aatatccatg ttttgataaa atttcatttc ctgttttaaa atcctcatca
     6061 ctagaatttt tcagtgattt tagtatactt tctgtagatt taggattgta ctcatatgat
     6121 gcatcaagaa ccttagacag cacattgtat tcattaaaag aagtgatagt tgttagaatt
     6181 acactcaata aaaaagttac aattaagcag aatattataa aaacaactgt actgttgata
     6241 tttcttggag ttttcactag ctgtcctcca ttctatatcc tatacctctt aatgttttta
     6301 tacaaggtgg attttttaat ttttctctaa gacgctttat tgtaacagta agagtgttgt
     6361 catttacgaa attaccatct acatcccaga gcatttctag taattttgta cgagtcatag
     6421 ctctattttt attttccata agacttagaa gaagcttata ttctcctgct gtcagaggta
     6481 cttcaacact atctaaatat gccttgactt caactttatt caaacttata tttccacaag
     6541 taatagtatt attagtatta gaatgagctc ttctaagaac tgcattaatt ctagattgaa
     6601 gtactgaaag caaaaatggt ttaaccacat agtcatctgc acccatatct aatccacgta
     6661 taatgtcttt ttcatcatca cggactgtta aaaatatcac tggagtatct tctatacttt
     6721 taacgtattg gcaaaattcg tatccaatac catcaggtag atttaagtca agcagaatta
     6781 aatcagtagt tttatcaaaa ttttttttag cttcagcaat cgtattataa caacatacaa
     6841 tgtgattttt attttccagg gaagtcttta tcccaaacgt aattgtatca tcatcttcta
     6901 agagaagtat tttagccatt tacttttagt cctttcatta atttaaatta ttagttttat
     6961 gcttaatatt ttttatatta tatatagtaa agattaccat ttaagtatat aaattttaat
     7021 ttatatactt aggtctatgc tttaatcgag tctattaaaa tggcaagggt tgtttcaata
     7081 aatttttcct catctatatt aaaattgttc aggtatgatt ttcttgttac agacaataaa
     7141 ttaaaaaatc ctgtaagtgt aaatattgat gaatacataa gagtagatat ttcaatatca
     7201 tacctaatac ttccatcatt ttttccaatt tcaaacattt cttgtatttc atcaaacata
     7261 aatttattaa aagaaaaaaa cttttcttta aacagcatct cagtatccga ttttttagag
     7321 tttacaatac caatcattcc cataaggcat aatagagaag gttctacaga gtaaaattta
     7381 tgaaaagcaa agtatgaaag ttttattttt tcaaaccctg ttttaccttt tgcactttct
     7441 attttaacat tatcccaaag tcttttatat ccccttaaga caacagcaaa gaataaatct
     7501 tctttacatg aaaagtactt gtatatggtg cgtttagtat attcgacttc ctttgccaac
     7561 tcattcatag ttgtattatc aaatccatta agagaaaata atttttctgc tttatcaata
     7621 atttcatttt ctctttgtat tttttcttta tctctttttg ataattctga catataaatt
     7681 attcctttct attgataata aagtatactg gtggtatact tgtgtaaata taatatattt
     7741 gtatactatt agtatacttt aatgaaaata aaattaatat caagtattat agctatttat
     7801 aacttcatta attaattata taggcaaaat accaaatagg gggaatttat ttatgaaaat
     7861 aaaagaaaat gtattaaggt tagatagtac tcgtggctca aattgtttta ttgtgtttga
     7921 taaagagatt actcttatag acacaggtct tccttttatg gggaaaaata taattaaaga
     7981 attaaaaaca cttggtatca aacttacaga tattaagcat attttattaa ctcatcatga
     8041 tgttgaccat atgtctaata ttaaattatt aaaagagaaa acaggagcaa aggtatgggc
     8101 tcatacagaa gatatacctt ttattatagg agagaaagat agacctgggt ttaagaagtt
     8161 tattggaaag acaatttcaa gaaggctcat taaggatgtt gaaccttatg gagagaatat
     8221 gcagattggg aatataaaaa ttatccatac tcctggtcat actccagggc atgtctgcat
     8281 gatttttgaa gatgtattat ttgcaggtga tttagtcaaa aacaagaatg gaaatatagt
     8341 ttcttaccct aatccctgga attgggattc taaaaagatg atgaaatcta taagagaatt
     8401 ggataattta gaatatgaat ggatatgtat gtctcatgga actccttgta ttaaataata
     8461 aaaataataa gttgtaatat atcctatgtt atctattgac tatatccaag attggtgata
     8521 atataatatc caagatagag tataggtggt ggatttttat gatacgcact ttaattttat
     8581 attatctaaa tattaagcca acacatgggt atgagattca aaagttttta caagtatctg
     8641 gtgcagatag atggactaaa atacaatctg ggtcaatata ttatgcattg gcaaaattag
     8701 aaaaagatgg aggcgttaga gttttaaaag aagaaaaaac aggagctagg attcgtaaaa
     8761 tctatgagat tactcaaagt ggtaaattag aattaaggga agaaatacaa aaagagcttc
     8821 agatgccaat agttccaacc ggttcaaata aattcctatt acataatatt ttggatgtat
     8881 tacctaagga tactttgcaa aaaaatcttg aaaaacatat aaaatatctt atagagcaga
     8941 agaagtattg ggaaaattgg aaagagatta aaaaaattga taaaaagagt cttgcaacag
     9001 aaaaaattgc atttgatatg actatagata atctgaatta tcaaatttta tggcatgaag
     9061 aaatattaaa tgatattgat aagtatatat ccgttggttg cgagacacag aatataatta
     9121 aatctataga tttttcaaat atagaagaag attttttatt tacatctgat acgacaaatg
     9181 agttattaga agttcagaga cttagagatg aaataattaa taacccagat aaagctatag
     9241 aaaacataga taagataata ctaaaattac aaaataagta ataaatttta ttacaataac
     9301 acgggtaaaa tactcgtgtt ttaaaaaaat tatatataca atattggata tataatgttg
     9361 gttaaatatt ggggggttat aaatgagtaa tatattgtta gagattaaaa atttatctaa
     9421 gaagtataac aataaggaag ttgtgtcaat agaaagtttt aagttattgg aaggcgatat
     9481 tttaggtttc attggagcaa atggagcagg gaaaagtaca tctatcaata tgcttactac
     9541 gattgtgtct ccagatagtg gaagcatata ctataaagga aaagatatta agaatcaaca
     9601 aaatattttt aaaagttcac ttggagttgt accacaagaa ttagcaatat atgaagattt
     9661 aagtgcatat gataatgtta aatttttttg ctcattatat ggatttaaag ggaatgaact
     9721 taaagagaga actaaagatg ctttggaatt tgttggtctt tgggaaagcc ataaggagtt
     9781 tccatcaaaa ttttctggtg gaatgaaacg tagactaaac atagcttgtg caattgcaca
     9841 tagtccaaag attctcatca tggatgagcc aacagtaggt atcgaccctc aatcaagaaa
     9901 taaaattttg gaggcaatca aatgcattaa tactaatggt acgacagtta tatatactag
     9961 ccattatatg gaagaaattg aaaccttatg taatagaatt gctttaatag ataaaggaat
    10021 aattatagaa gatttagaca agagtttata taaaaataaa tatttagaat ttggttgtaa
    10081 gacattagaa gatatttttt tatatcttac aggtacagat ttgagagact tggaggaata
    10141 aatagatgag atgtttttta ccattgttta aattaggaat aaaacgaaga agtaaagatt
    10201 tttttattct gttttacaat attgtttttc cagtaataat cataatgtta ttaggatatt
    10261 tgacatcaaa gtcctatggt gaggaattta catcatataa ttattatact attgttacaa
    10321 taccattttg tgttcttatg ggaattatta gtgtttcata tgcgggacaa gatgaaaaaa
    10381 tttctaacac atcatatcgt tatatgattt ctcctatttc aatagtagat ttagttattt
    10441 caaaactttt ttcttgtaca gcagtacttt cgatatgcaa tttggtaact ttagtggtgt
    10501 taaaatgtat ttttcgtatg agttttggtg gaaaattttt agctgttctt ttctttttaa
    10561 tgtgtgaatc tattgtggta gtatctatgg ggttgttttt gggtcttgtt tgtaaaaatt
    10621 tagatatgtt acgtaatatt attaatatac cgatagttat ttttggattc ttaggaggag
    10681 tgttctttcc agttagttca ttgaatccag ttatttcatt aatcattaat atatcaccat
    10741 taacatggat taatagaggc atagttgctt gtatctatga taataattta caactccttt
    10801 ttaatatatc atgcatattt gtagttgtaa gcatatttat gacaatactt acaatcaagt
    10861 tttttaaaaa gggggtcttt atatagtgaa tgtaataaat gtttttaata ttaacttaaa
    10921 aagaaatata aataagagaa tagtattttt agttacactt ttatttccta taattatagt
    10981 aattttggga gtattggcaa attacataag taaaccattt tttaatattg gtattttaga
    11041 tgagtacaaa aacgaacgag ttctaaagga atctgaaaaa agaacctatg aaaatacaat
    11101 aatggcttta gaaaatactc aagggattaa tacaaagatt gcagatttta aaacaattaa
    11161 aactgatatt ataacaggca agtattcagc tgtcatatat tttaataaga atggttttaa
    11221 gttatattct ataaaaaata aaaatacgaa tgatacatta aagtatttaa tagaaacata
    11281 taaaaaagac ccagttcctg tagatataag taaattacaa gaaaatactt taggagtttt
    11341 acaaagaata attgcattta tcgtattatt tttaatgata acatctactg taacagcatc
    11401 aatgattatt aaagataaaa actcaggtac atttacaaga gtgctgtatt ctccacaaaa
    11461 tgtaagagga tatgtgctag ggaatatgtt atacaacttt acaataacgt attttcagta
    11521 catagtttca atatctataa ttaagttatt taatattgat attggtatgg attatataga
    11581 tttaatttta atgggaatat gggtagtagc tttggcaact tcttttggaa catttgtatc
    11641 ttctttattc aatagagaga tgcatgcaaa tttattttct acatgtatat cacttgtatt
    11701 atcacttatt ggaggttcat tcatctcagt tgaaaaaatg ccagaaatgt tgcaaaaaat
    11761 tagtattata agccctgtaa ggtggtttat tagttttgtg acttatatag aagaaagaga
    11821 tggaagcttt tttaatatga attatatttg tattttgacc tctataataa tagttttagc
    11881 tttgttagct atgaaattta ctcaaagaag taaatattca agtaatagta attattaaat
    11941 aagataaaaa ataaatatat gtaattaaaa caaatcaaga gataaaaata taaatatggt
    12001 tctatatctt ataaattggg tttaattata aaaatagaaa ttatatagtg taaaaaatag
    12061 tgtgaaaatt tttttatttt ataatatagt gtcacatgag caaattaaat ttgttctgag
    12121 atagctcttt tttatttatc ttaatagaaa tatttttaat tgttttatat aaagtttatc
    12181 ttctaaataa aattatgtat aacaaataag ggtataaaaa tactattttc tagggacaat
    12241 ttgatagata taaagatctt ttgagacaac atagattaaa atgtattaaa cacttgttaa
    12301 aagtaagaat tgaatattta aaagagaggg gagttttata tgaataattt attttatgat
    12361 aagctaaatt ttgatgataa actaatcatc aaaaataatg gagtaatcag aaataacgac
    12421 ttatattttt taaaatataa aaacgatacg gcaggaatct taatatataa tataataaat
    12481 gataaattct catttaagca aaataaaaat tacaagggaa tgaacccatt tccaatcaca
    12541 aatattttca agaatttaga tagtgataaa tgggatgaat ttataagaat ttggattgag
    12601 gaaagagtta ttcaagaatg cagaccagag gttcctttta tactaaaaga atttaaacta
    12661 ggatattatg acgtttggga tatttgtaga agtaatcttg ctatgagtgt agaagatttt
    12721 ttctggatta caaaagataa cactctaaga tatgaagatt ttaacatccg ttattgtact
    12781 ttaaataata ttactcctat ttataagcct ccttttccta ttgttatata tccaaattat
    12841 tttgagagag aaaaaataaa aagtggtgaa acctggaaat atgaactaaa aaatactaat
    12901 tcttaaacgt ttttagatta tacattctta tttgtactat aaaaaataat gttaataatt
    12961 aaaactgtta ttaagtgatt ataaatcatt ttaataacag tttttttgat tttttataaa
    13021 aattttcaaa aaagacttct aaatgcccca tattatatac aaattaaatt aaagctatac
    13081 tttagcaaat aaaaataagc tgtaactatt agcataaaaa ataaaaggag gtttttatga
    13141 aatcaaaaac tttatcttta ctctatcaaa aaaattaggt gaaacattta aaaactttaa
    13201 cgaaattaac aaacaaaata ttcttaaaac aaggagattt ttattcaatc taggatttga
    13261 agtaaaatat ccagctggaa caaacactgt tttaaaaaaa tttaaatcta aggaaaataa
    13321 tgaagaaaaa gaagttacat tattttactc aaaattagat agtacaaagg atatttaaat
    13381 attgattata tctgtagcat catgaatcaa tcatatcata atatcaaaaa tgaattatta
    13441 tctaaacaac ttgcttatat tgaccgtttg acaggatatt tagtagatag agataattat
    13501 ttgagtggat atgttaaaga aaaattaaaa atagccaaag aagcatcaat aaaagataat
    13561 actttcctgg ttaatgttaa agctttagaa gctaatcaac ctaaatatga aacagatgtg
    13621 ttctttagca ttgattctac ttggatatct tcaagtataa aatctgattt tattgaaaaa
    13681 acattagaac tcaaagaaaa ttcaattcaa ttattgtatt caacacaact tggttattca
    13741 gtaaaatgtt ctgacattat acctataagt aaaaatgaat ttacatgggg tacaaaaagg
    13801 agaaaatctt tgtatattgt tgaagcagaa ttaaatatga aaactataac tgtcactgat
    13861 aaagaatatc aaaatggtaa agaagtttct gttaaaaatt tggaagaaac tcaacttgct
    13921 atgaacatat taaataaatg gcttatagct tttcataact atgtaacaag taatagttat
    13981 attcataaag aattacttga tagatacaat gaaatgttta tagattataa ggaaaaggaa
    14041 tataaaaata tattaaatga tgttagaata aatcctaata taaaattacg agaacatcaa
    14101 ttaaaagcgg ctagtagaat aattacttct gatcgtaata ctcttctatg tcatagtgtt
    14161 ggttctggaa aaacttatac aatgataaca gctgcacaag aattaaatag aatttctaaa
    14221 atagattcaa agaaaggtaa taagaattta tttgttatac caaattcact ttgtgaatct
    14281 ggacaatttg ctaaagagta ttttaattta tatcctcaag cgaatatatt agctacaact
    14341 tcaaaggatt tctctaaatc caatagaaga aaaattatag ctaaaattgt gacatgcaac
    14401 tgggatagta taataattcc acattcggta ttaggattaa tccctttaga agctgataca
    14461 gaattaaaat tattagactt atacttaaaa gaattaaatg atactcttaa atattacaaa
    14521 aaagaatgcc ctgatctttc tacaaaacga cttgaaacaa tgaaagaaaa ttatctaaat
    14581 agaatcaaag agctaaatga tatgcataaa gacgatgctc ttctttcatt tgaaaagctt
    14641 ggaataacaa acttattcat agatgaagct cacaacttca aaaatttata ttttaataca
    14701 actatgcaag tccctggtat atcaacttta aaaactaaaa aaacacagga tttatacaac
    14761 aaactaagat attttaaatc tattcatgga aataaaggtg ttacatttgc cacagctaca
    14821 cctatttcta atagtatatg tgaaatgtat actttactca cttatttaaa taaagataca
    14881 ttaaagtatt acaaagtaga tactttcaat gcttgggcaa gtaaatttgg ttcaattata
    14941 tcctcaatgg aagtagatcc tactggtcaa gggtttagat ttcagcaaag atttgctaaa
    15001 ttctgtaatg ttccagaatt agttaatata ttcagacaaa ttgctgatgt agttaacatc
    15061 tgtgacataa atgatttaga tattcctcag ataaaaggtg gaaaacctat aatagaaatt
    15121 ataaaaccta caaaccaaat gaaagaatat atagattctc ttgtaaaacg tgcagaaaaa
    15181 attcatgaag gcccatatga tccaataata gataacatgt taaatgtaac tacagatggt
    15241 cgtttgatgg ctgtaagccc tgcattagtt ggaattgatg gtatattacc aaagataata
    15301 aaagttggag aaaatatagc ttctttatat catgaggata aaataagtac acatctagta
    15361 ttctgtgatc taggaactcc taatggaaat tcacataatg tttataatga tataaaaaat
    15421 gaacttctaa aaaataatgt tttagaaaaa gatatagcat acatacatga cgctaatact
    15481 ccagaaaaaa gaaagaaact tattgatgat ttcaataatg gtattattag aatattaatt
    15541 ggttctacct ctaaaatcac ttcatcatgt agatgttcct tggaaacctt catcaatttc
    15601 acaaagagaa ggtagaatat taaggcaagg aaatttgaat aaagaggtct atatttttag
    15661 gtatgtagtt gaaggttctt ttgatgcata ttcgtgacag acaatagaaa taaaggcaaa
    15721 atatatagct caaattctta ataattcttc atcttctagg actgctgaag acataggtaa
    15781 tcagcttatg agttatgctg aaacaaaggc atgtgcctgt tctgatgata gcatattaaa
    15841 attatgtgag ataaatagag aaattcagat aattgaaata aataaaaaag cctttttaaa
    15901 tcaaaattta cttactttaa atgacataaa atcaaccgaa aattcaatag atgctttaaa
    15961 gatatcaatt tctaaattag aagaagatat taaaacgtat ttttctaatt ttttttaatg
    16021 aatttaagat aacattagat aatgaaactt atattgatat taaagatgct atagaagctc
    16081 tgtcagatat aataaataat ggaaaagtag gaattttagg tgaaatatat ggcttgaaca
    16141 taatttatga aaaagaattt acatttgaca ataaagttag agaattcatt tctctaggta
    16201 ataactataa atttgaattt tctacatcaa aaagtcctaa aacactaatt aaatatttca
    16261 tggattataa taaaattagt tcaaataaat tagaaagttt taaagaaaaa tattcattta
    16321 agaaaaatga actcttatct ctccaagaaa ttttaaagaa taagtttaca gaagatgata
    16381 aattagaatc tcttaaactt tcaaaagcaa atcttgagaa tgaattaaaa ttaggaaatt
    16441 ctataattaa aaaaagcaac tcaaatcata atatggtaga aaaaaaatga attaatattg
    16501 caattgtttt aaaattgttt tatgcatgtg tagtataaaa ttccaaaata aattgagtat
    16561 tttcaatact tttagataca gaaagtattg aaatttgtaa tgagcacaaa gtggtgtttt
    16621 ttatattaca aaaaagacct tgtaactcaa tatctgcaac ccttttaatt aaaactttaa
    16681 aatatatgaa atcagaaaaa tgattttagt ctatttatta gatattcttc tctctcaatg
    16741 cttaaagctt ttagatatat aagactatcc tgtttatttg cacctgttat atctttaaat
    16801 ttaatatagt ttttaatatt cttgcctcta aaaatatatt tattatcaat caaaatatca
    16861 tcatctgtaa ttgaaatata aagtttattt ttatatttta ttaaaattta tacgtttgtt
    16921 caagataaaa acctgcaagt aacaaaaata gaaaagttac tttgaatata catattattg
    16981 aaaatcactc ctacaattta tattgaccta tgttattcaa aatactttac tctacttgca
    17041 ttaaagatgt aatattagtg aactaaatta atataatttt ccattcaaaa cgtgttattt
    17101 atattcacat aattacaata gaaataccta taaggattat tccaataata cataataaaa
    17161 gtgtctccat tagattttta ttaagcttat aagaattata agctagtaaa atttgttcca
    17221 ctccaaataa tattaaacaa aattttaata aattaaaatt tttaataata ttagtacaag
    17281 aaagtgctga tattaaaact attaagatag aaattaataa agttaatatt ttaatttgct
    17341 tgttatagaa catactattt tccccctcat tttaaatgca tactataatt ataaacaaat
    17401 agaatttatt caagtaaaat atttctcact atttttaata tgataaatca aaagagaaaa
    17461 aagcacctgt acttacagat actttttttc tgatttcttt taatgctcct aaatatgttt
    17521 tttcgttttt gtttatctgg gataataaat tcataatttt ttcattagtt aaaatttgag
    17581 tactagaaat atttgtgtct ctcatcaacg ctatgtaacc tgtaataata gctgttgctt
    17641 ctgatgttcc ctttccatag aaaacattat tgttattatc tatcaccttt attttttctc
    17701 caggtgcatt tattaatgtc ttttgaggtg catttgtcaa tgttgaaata tctcttctat
    17761 catctatgct accaacagaa ataacattat ttaaagatgc tggaaatagc aaaaatcctt
    17821 gtttataatc acctgctgaa cttacaactg ttatattttt aagattagct ttatctattt
    17881 tttgttttat attaatatct gtttttgtgg atcctaaact taaactaata atatctactt
    17941 tttcatttaa tgcatagtct atagcatctt ctattacatt aggattaata ctttcatcat
    18001 cattcattac tttaattaat attattctag aatctggaga tattccttca atcgtttgat
    18061 tatttttatt ataacctttt atgattgaat acatcatcgt tccatgacca tttgtatcat
    18121 aatcattatt gctattagtt aaatttatat ttttataaac cttttgaaat tttgaaatac
    18181 cagaatctat caatgctatt ttttgacttt tcccactaga tatttcatgc atatcactat
    18241 aattcatata attaacatac caacagtcag attttatatt attaaaaact ataaatatag
    18301 ataaaattat taaacaagat ataaatatta ttgttttaag attttttttt tataatttaa
    18361 gttttgtttt tcactcatta atttcaccta atttttactg attttttact gcataagtta
    18421 accaccagtt tatatccaca gttattcctc tattatttcg tgaatttaca taagatactt
    18481 tatttgcaag acctcccata tatgcagctg ataaaccaga tacaaatcct attggtgctc
    18541 ccatagaacc taaaagaaca cttactgctc ctgttgctac aaatccttgt gaagacttat
    18601 tcaaatcata aacccatcta tttgctgctg cagtactttt attttgtctt actccccatg
    18661 aaaatttttg agtatatgta gaacctcctt gtacataaaa tgagttgtct gtattgttta
    18721 taatatcttc ctcaggtgta attttaagag ttttattttt tactccttca tttatatcat
    18781 ctatattttc tttaactaaa ctatatatta cgtcactatt aaaattattt atgaattttg
    18841 aatcaattcc ttgctctatc atttgttctt taatatcttc taagttaatt tctttcaaat
    18901 catttttcaa gtcagaagaa acttcaatac cctctttggt ataagataca tttgattcta
    18961 taacatttat tatttggtta tttacatttg tgtctttttt ttctaatgca ttgattgaaa
    19021 ttactggaat aagcaatgca attgatagtc ctaatgcaat tactttttta aaatatttca
    19081 taataatatt cctcctaaat tttaattaaa ttaataattt gttacattac ttgttatatt
    19141 cctactttca tttccagctt ttattgttgt taatattcta taagaatacc cctttgatac
    19201 tctatattct ctagataaag tacttgaagt ttttcctgag cttgaccatg ttgatatagt
    19261 tacccactta ccagattttt tttgctgtag tcttgctgta attgatgtta aactcccaat
    19321 tttaccatca acataacaag atatatgagc ctttccagaa cgaatactca aattactacc
    19381 agcaccttta atataaatca taaataaatc attatcattt gtttgattat aatcattagt
    19441 atttaatgaa gtatcttcaa aagcttgaac atttaaaggt aataggcaag ttaatgtaca
    19501 taatcctgct acaatcactt tttttaaata cacaaatatc acctcctttt ttcttatata
    19561 ctcatataac gaaaaaaaag gtgatttgtg acactttttg aaaaaaatta ttttatattt
    19621 tcagcaatat ctataagatt atctttatac ttatttaaat catttttatt atcataatca
    19681 aaatatattg tatatacatt ttcttcatca ttccaaaaaa ttgtaatact attgtcttta
    19741 acaatatatt tagcttcata atcatttacc ataatatctg tgacttttgc attctctgta
    19801 tctaatatta ctgtatttga atcaatttga ttggaacgat atcttatttc agagccattc
    19861 ttgttttcat agcgaataaa gataccttgt aatgtgtttt ctttttcaac ctctttgaaa
    19921 ccatttggta tatatttggg ttcttttaat ataaaatcaa gattcttatc agtttgaata
    19981 tctttagaaa aaataaattc tgttaaatct tcataaattt ctattacaat tttgataaat
    20041 tgatttctaa gggtatcaac actcatagtt attgtgaata atccagttat aattattaaa
    20101 aacacaactg caactcgttt taaataaata atagtatatt taataaagaa gttactcaaa
    20161 tatcttttga aatagatata ttaaattggc taaataaatg tgtctcacat catgaaacta
    20221 taaaaatagc tccaataaga gaaattatac tgcaatttat ggaggtaata agaaatatga
    20281 caaatgtatc atcactagaa aaagaggatg agatagtaga aattttatca tctaaagaaa
    20341 atataagagt agcaatagaa attgaaagag gattaaagtt atgtaaaaca caaatgataa
    20401 aaaaggtttt agaagaaatt gaaaaacgta tggataagaa atttgaagat aaatataaat
    20461 taccatatta ctcatacaaa gaaaataatt atgctttagt gaataattat tataataaga
    20521 aaagctctac atatccagca attaattatt ttataaaaag tctagataaa gaagatgttg
    20581 atcttttact aagaattgaa atagatcatc atatatttgt aggtttttgt acgttatata
    20641 aagaaaaacc tagcggaaag attctaagtg atgatgaaat aaaagaatta ataaatgatg
    20701 atggttcacg tactaatgga tggtggattt gttgggaata catttataat aatactatgg
    20761 aatgtcctaa tttcaaaaat tttaatgatg cttattttga tttatttgat gataataaat
    20821 ttgatgaatt tatggattta tgtgaaaaaa gaattttgtc aatattaggt aaactaaaag
    20881 ataagcaatg cataaatact tttatctaaa atagaatatt acctaacaag gtatgtaaat
    20941 gtataagact caaggatttt gataacaaat aagagattac atgatttgaa gatatgatgg
    21001 aagaatttag atatagttta acaattaata gataatagac tgaaaaggat ggtaattcta
    21061 tgaagattga aatgggagaa tcactgttgt attcttggct acgtcatgtt aaagaatgcc
    21121 aaatagtgca aacaaactgg aaagtgtcat ctaaatggga tttaaagaat gctgataaaa
    21181 tactagaaat tatggaaaaa actaaacaat attttgagca gaaatatgga tatcctatat
    21241 ataaaaataa ttcattggag caattaatta tgcaagctga agttgatgta ttaggtattt
    21301 caatagataa tggaagaaat catatttatg ctatagatgt agcttttcat gaagcaggac
    21361 ttaattatgg atctaaaaat gaaactgtta caagggttat aaagaaatgt atccgtacag
    21421 caatgtgtat ttatggttat atggacttag atgaagcaga aattatattt gcatctccaa
    21481 agattaatcc agctattata actgaattag aacctaagat ggcagaagtc aatgacctat
    21541 ttaagtcttt aggattgaga tttaaaacta gattaattgc gaatgaagaa tttaatggta
    21601 aaatcctaga accaatctta attgctagtg atggaatctc tgacacatca gaacttttcc
    21661 taagaagtta tcaaatgtac agtatgtttt catatagtcg agatgttgta aaacaacaaa
    21721 gaataaaaaa atctaattct ttaaaagatg cagttgaatt aaatttgtca gatgaaacat
    21781 taaaggaatt aaagattggg aaattagttc aagtaactat gaaacatctt tttgaagaag
    21841 aaaaaattag ccctgaagaa attgataaga tgacacaatc cagttattca aagcaagtgt
    21901 ttaatgaaaa taaacctgta ctaaaagaag ttcctaaaga tggtaatatt aatgaattga
    21961 aaaaagatga aaaaaactac aatagatatt atgcttacac tatttccatt tatggaaagc
    22021 aatatttatt agtatcacaa tggatagaag atttacatcg ttcatattat gaaagttggg
    22081 ttatcaaata aattataatg ctcttgctaa atctaagaaa aatgcaataa cataaaagtt
    22141 aaatttaata aaatagttag ttgtacatta aacattttta acttaatgta ttcatcttct
    22201 attacacaca tttttatatt aaaagaatac aaaatataac ttattaagta aattatttag
    22261 gcatttaaaa agccactccg gcaagagtgg ctaggaattc gatttaaaaa tcaagttcaa
    22321 aaaatttaaa taatttttta ttttaaatta cgaactcaat tctgtcacta aatagaatat
    22381 agttctcttt cttttactgt tataacacaa aaccttaatt ttgatacaat tttatattgg
    22441 attgccaaaa ctccctattc cagggttatt aaatataaaa gttttttata taaactttaa
    22501 gatagtataa aaaataagag gtattaatct cttgtttttt tatg
//
LOCUS       DAMJVM010000237        21293 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.guided.061, whole genome shotgun sequence.
ACCESSION   DAMJVM010000237 DAMJVM010000000
VERSION     DAMJVM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21293
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.guided.061"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            824..1225
                     /locus_tag="PC523_003585"
     CDS             824..1225
                     /locus_tag="PC523_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HDF2477035.1"
                     /translation="MNYKNRKRAYYQENSCLDNFICKNCGCIVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNP
                     VKLMSIALKPLAQPPFPLERITEKMEKEETE"
     gene            1222..2289
                     /gene="rsgA"
                     /locus_tag="PC523_003586"
     CDS             1222..2289
                     /gene="rsgA"
                     /locus_tag="PC523_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF2477036.1"
                     /translation="MNGMKEIDLQDYGLNSNFITQSMKYHDLYIARIISQEKNLYQAV
                     CTGGIVFAEVSGKLRFEATIPSDFPTVGDFVILDRCNDKNGNAIIHHVLHRKSLFVRK
                     AAGKANEEQVVAANIDIVFLCMSLNKDFNTHRLERYLSLTWESGAVPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSAKESDGCQQISKYIKPGYTIALLGSSGVGKSSLINC
                     LLGGEFLNTKEIRIADDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGVDKS
                     FADIEAYFGKCHFRNCSHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKTIRR"
     gene            2832..3530
                     /locus_tag="PC523_003587"
     CDS             2832..3530
                     /locus_tag="PC523_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2477037.1"
                     /translation="MKISSQYRSQYSFRHESNINNTRINESIVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKDRQEVYTKKINEAIKNLYKSEIRETTNNFSNIEIGIKNSIIED
                     KNKSTMLYENSTYLNTSDEKESLITKEFNEKVEEEMLNDEKLEELEQKKDYKEDSNKK
                     EKISEDLSLAGKTREELENMLKNFTNLTQEEIMKLESRIEKLDKDAEEYKQNSKTNIF
                     DKTDEQKKHINVLL"
     gene            complement(4109..5065)
                     /locus_tag="PC523_003588"
     CDS             complement(4109..5065)
                     /locus_tag="PC523_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2477038.1"
                     /translation="MKNNTTQKRLRDLSKKIWNIYILNPEEEGIKFIISLLDDNLTLI
                     GTGKHEFYETLEEFIIGLEKDQVESNQIDFEIIDEWYSCTKVTDETFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVKDDIIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHI
                     RGDEILIHFSNILTKVFISDAIIGRLGGDEFAVFEYSNQSKEDVENKAKKVLELCLEN
                     NSCSIGISRLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            5768..6763
                     /locus_tag="PC523_003589"
     CDS             5768..6763
                     /locus_tag="PC523_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2477039.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFIGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAIV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSEYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAVAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEELFNHFQKYKKITVSFSNETNIVFSKDCKVVEQT
                     NHKTSVVVPKERGNETIKWIFDRYDVKDVMIEEEDIVNIVERIYKKGSDEYYEQL"
     gene            6741..7604
                     /locus_tag="PC523_003590"
     CDS             6741..7604
                     /locus_tag="PC523_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF2477040.1"
                     /translation="MSIMSNYNEASSNSSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMMFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLSGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKINFAFT
                     SNPIQILISIFMIFLGLIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPL
                     VLLPETIVKVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            7601..8395
                     /locus_tag="PC523_003591"
     CDS             7601..8395
                     /locus_tag="PC523_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF2477041.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPIDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVIIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFSFTLFMWRFWKLGLRHYKSASS"
     gene            8678..10057
                     /locus_tag="PC523_003592"
     CDS             8678..10057
                     /locus_tag="PC523_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2477042.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCHSYIFIILIFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTISGMVFFSKNKGVLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLISEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNMLNINGIWLAVPSAEILTLFLTVFCIC
                     KHKNRYKYM"
     gene            10507..11220
                     /locus_tag="PC523_003593"
     CDS             10507..11220
                     /locus_tag="PC523_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2477043.1"
                     /translation="MDTYFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIVFRVKAQLRRQQYQSIVPSDSSVIKIGDITIDIEGNRVYKDRKEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(11497..13035)
                     /locus_tag="PC523_003594"
     CDS             complement(11497..13035)
                     /locus_tag="PC523_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2477044.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVIPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQESAISGNEQHLIKASDYQEQLIANNFYSIDNEVNKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHEVLLLDEPTNFLDKEHVEWLSNYLNTFSGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKLHLREDYIRRYQSQQKKIEKEEAFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPGFTGKPNIKFSEIEISAQNALTVTNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPCISGDFQFSEQVKLAYYEQDL
                     KWENPTKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLFPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            14043..14329
                     /locus_tag="PC523_003595"
                     /pseudo
     CDS             14043..14329
                     /locus_tag="PC523_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            14317..14643
                     /locus_tag="PC523_003596"
     CDS             14317..14643
                     /locus_tag="PC523_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HDF2477045.1"
                     /translation="MSSYLNLIREFNLKEKIIAFICVSALLIGKSCVLKNRKATTYHL
                     KNSKRQRQLSEFDVNVVNEPTVVNQNIITSYYTETAPHVAFKLLEMLISKKQLDEVKL
                     AMGFKL"
     gene            complement(14648..14971)
                     /locus_tag="PC523_003597"
     CDS             complement(14648..14971)
                     /locus_tag="PC523_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2477046.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLEKL"
     gene            15123..15641
                     /locus_tag="PC523_003598"
     CDS             15123..15641
                     /locus_tag="PC523_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2477047.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLDKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGLNKLSKAANIYDAPLAILVCGDKEKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKVADSPDRHEKTR
                     VPLSEIVSYEIL"
     gene            complement(15832..16500)
                     /locus_tag="PC523_003599"
     CDS             complement(15832..16500)
                     /locus_tag="PC523_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2477048.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLILKNSTIKN
                     IIEKHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKIELPLKIDLANGSEHFYGIGAKEQILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKYYISSAFPDLKINS
                     IDIF"
     gene            16615..18030
                     /locus_tag="PC523_003600"
     CDS             16615..18030
                     /locus_tag="PC523_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2477049.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILNGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNYIFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRAEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGVKVDDKGINTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKYATENGYYII
                     EDDYDSEFRYEGQPISSLYELNPNKVIYIGSFSKILSPAIRVGFILLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIEKILEYDHEIILGYAHLS
                     FDEIEKGVKILSKIIHINQGT"
     gene            18253..18711
                     /locus_tag="PC523_003601"
     CDS             18253..18711
                     /locus_tag="PC523_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2477050.1"
                     /translation="MEIIDYIEENLTENLNLDKIADIANYSKYHLHRMFTNIVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITAGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKYISYANMNCKQRIDEINSYLNFNINCEVKVA"
     gene            18708..19292
                     /gene="clpP"
                     /locus_tag="PC523_003602"
     CDS             18708..19292
                     /gene="clpP"
                     /locus_tag="PC523_003602"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDF2477051.1"
                     /translation="MSLVPHVIEQTSQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSVTAGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRYALPNAEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDRIFSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            19595..20671
                     /locus_tag="PC523_003603"
     CDS             19595..20671
                     /locus_tag="PC523_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2477052.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGSPTLSYYRERLFKRSSNHKWVDPIHEVVPLSGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKVLEPRHQFYYSRELYYNAKYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPYIQLSVCYDRLGESNKAIYYHEK
                     TKEIKPNDSAVLHNENYFNKFKNS"
     gene            20738..>21293
                     /gene="bclA3"
                     /locus_tag="PC523_003604"
     CDS             20738..>21293
                     /gene="bclA3"
                     /locus_tag="PC523_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HDF2477053.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNSGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGGTGRGGPT
                     GPTGPTGPTGPTGPIGFGITGPTGATGATGATGADGVTGPTGPTGATGANGITGPTGA
                     TGATGFGVTGPTGPTGATGVGVTGA"
BASE COUNT         7874 a         2480 c         3363 g         7576 t
ORIGIN      
        1 taaatttctc tagttttgcc gatttagttt tattctacaa ttacaataat acaatagaat
       61 tgattattat tcaatatact tgtttgaaaa ttaaataaat ttataaatat gaacgttatt
      121 ttatataaaa aaatatacta ttcatgtaag aatatatgga agagcttgat tatacgtttt
      181 gtttaaaaga cctttgttat taacttaaat tagataaatt gagggagaga tataagatga
      241 taaaagaaat aatagcggtt aatttttatg attaatattg aatgaatatt gaatttcaaa
      301 agattactag attctataga ataaaaaaat aagataaaca taaatatatg aatatgttaa
      361 attatacttg aacatactca tattttatga taaaatttca ataataatta ttagtaaagg
      421 agtgtcttgc ctggtgtcag agttgtatat aatggtaacc aacaaataaa tagttggagg
      481 cgcatatggg ttctgtttaa tcagaatttt taagtgtatt tttgatacat ttaattgttg
      541 tgtcttactc tgcctttata gataactaac attcgacaac aaataataat attacatatt
      601 actttgttaa attgtggtgt agtgggcttt aatctgctac acttttatta attttacata
      661 gtattatttt gaatatagtt atgttgaaat agaagatatt aaattttcta gtccacagga
      721 gttttctgtt tttgtgtagg caaatatata taccacacaa aaaggcagat ataaatacct
      781 gtggattttt ttgtataaaa cacaagatat ggaggcgata aaaatgaatt ataaaaatag
      841 aaaacgagct tattatcagg aaaattcctg tttggataac tttatttgca aaaactgtgg
      901 ttgcattgta gtacctgaag gagctggtac acaacatagg aatcattgtc cacattgctt
      961 acatagtctt catgtggata tcattcctgg tgaccgtgct gcagattgtg atgctaatat
     1021 ggaacctatt ggagtgtggg tgagaaaaaa tggcgaatgg gcaattattc atcgttgtac
     1081 acgatgtgga catcttagtt ctaaccgtat tgcagctgat gataatccag tgaaattaat
     1141 gtcaattgca ttaaaaccac tggcacaacc acctttccca ctagagagaa tcactgaaaa
     1201 gatggagaag gaggaaacag aatgaatggt atgaaagaga ttgatttaca agattatggt
     1261 ctaaatagta attttattac tcagagtatg aaatatcatg atttgtacat tgctcgcatt
     1321 atatcacagg aaaagaacct ttatcaagct gtatgtacag gtggtatagt ttttgcagag
     1381 gtttcaggaa aactacgttt tgaagctact ataccatctg attttccaac agttggtgat
     1441 tttgttatac ttgaccgttg taatgataaa aatggtaatg ctattattca tcatgtgctt
     1501 catagaaaaa gcttgtttgt gcgtaaggct gctggaaagg caaatgagga acaggtagtt
     1561 gcagctaaca ttgacattgt ttttttgtgt atgtcactga ataaagattt taacactcat
     1621 cgattagaga gatatttgtc tttaacatgg gaaagtggag ctgtcccggt agtagtactt
     1681 accaaatcgg acttatgtga caatgtacat cgtaaattac aagtagttaa ttctatagcc
     1741 ttgggtgtag atgtagttgt tacatcagct aaagagtcag atggttgtca acaaatttct
     1801 aagtatatca aaccaggtta tactattgct ctgcttggtt cttcaggagt tggaaaatct
     1861 tcattaatta attgtctgct gggtggagaa tttttaaata caaaggaaat tcgcatagct
     1921 gatgataaag gaagacatgc cacaactaga cgagagttat ttttaatttc aggtggtggt
     1981 attgttattg acacaccagg tatgagagag ttgggaatgt gggatgtttc ggaaggtgta
     2041 gataaaagtt ttgcagatat agaagcttat ttcggaaaat gtcattttcg taattgttct
     2101 catacttcag aaccaggctg tgctatttgg gaagctattg ctcatcaaga actttcgcct
     2161 aaaagatggg aatcctatcg taggcttaaa actgaggcag tatttgcaga caataaaagc
     2221 gaatacttag caaaaaaaga aaagaaattt aaaaatatct caaaaataaa taagacgatt
     2281 agaagatgag tgtagttttt ttgttctgtt cgttctatga ctctatatat tattaatata
     2341 ttagatttgc catcaattta ttactttaat gtgattttga tggcaaattt gatttatggt
     2401 attatttaag gcaatttatt aattttttta ataaagtttg actttttata taatgatata
     2461 tacatatgat aatgtataca tgtttgttat gataggttct tttataaaga gttcttatgt
     2521 gagataagaa gaggcatatt tatactaata cataatagag tatatgctta tgtatgatag
     2581 agatgtttct taaaagcaaa actgtaatat tgataatttc taaagtaaaa atatataaat
     2641 tataaaatat aaataagata tttataaaaa ttattgtttt aatttataat aaaaattttt
     2701 tgttgaaaat aatgctcgaa cctatagaac tattaactaa tatttgtaaa tgtatacttt
     2761 tgatgataaa atataattaa gttaagttgg ctttttataa aacataaatt tgttttagag
     2821 aggtgaaata aatgaaaata tctagtcaat atagaagtca atattcattt agacatgaaa
     2881 gtaatataaa taatacaaga ataaatgaaa gtatagttaa gaaaaatgaa actgtaggaa
     2941 aagacactta tttatctaat attatgaaac aaaagcaaga acttaatgat agaattagag
     3001 atttaaaaga tagacaagag gtttatacta aaaaaataaa cgaagcaatt aagaacttat
     3061 ataaatcaga aataagagaa acaactaata acttttctaa tatagaaata ggtattaaaa
     3121 atagcattat agaagataaa aataaaagta caatgttata tgaaaattca acttatctaa
     3181 atacaagtga tgaaaaagaa tctttaatca ctaaagagtt taatgaaaaa gttgaagaag
     3241 aaatgttaaa tgatgaaaaa ttagaagagt tagaacaaaa aaaggattat aaagaggatt
     3301 ctaataaaaa agagaagata tctgaggact tatccttagc aggtaaaact cgtgaagagc
     3361 ttgaaaatat gcttaaaaat tttacaaatt taacacaaga agaaataatg aaacttgagt
     3421 cgagaataga aaagttagat aaagatgctg aagaatacaa acaaaattca aagactaata
     3481 tatttgataa aacagatgaa caaaaaaaac atataaatgt actgctttaa acaatatgtt
     3541 ttaataaata agtttattat attatgaaaa cataaaggta caatttcaat acacttctat
     3601 ttttggtgga acattagatt ttgattacct taagttaaag gatgcgacga gagagatgac
     3661 tacttactat gatgctaagg cattgtctat attaggattt aatcaataaa aatatggaag
     3721 gcttcttgtg gaatcttttt tttattaatt tgaaatttaa taaggaattt tatattaaat
     3781 ttaatcaata aaagtaaaaa aggctattta tagagccttt ttaagtattt ttttagtaga
     3841 attttttata tcagcatcag ttttaatcaa aaaatataat attttggtgt tatttttaaa
     3901 aataaaatta ttaaaatata atattaatca tgttaataat atttttagtt tataatattc
     3961 tataatagtg ggtatgatga ctattgtagc tctattaata atagttggtt tatataaata
     4021 tatgcaaaga taggttgttg tttaccttac ctattagtat gtaaaacaag atactctgag
     4081 aaatttgcct attttaatat cttttgcact aaaaccaact aaagtttcct ttattatttc
     4141 gttttgaata atataaagca taatccgatt gtttgtataa agattggaaa gtatcagagt
     4201 tattttctaa tctagatata cctatagaac aagaattatt ctctaagcac aattctaaaa
     4261 ctttttttgc tttattttca acatcctctt ttgattgatt actatattca aaaacagcaa
     4321 attcatctcc accaagtctt ccaataatag catcacttat gaagacttta gtcaaaatat
     4381 ttgaaaagtg tattagtatt tcatctcctc gaatatgacc aaagatatca tttacctgtt
     4441 taaaattatc taaatcaaag atataaaagt atccgtgtga aacggtttta agcatttctt
     4501 ttatgtgtac ttcaaaaaaa tgtctgttgt atagtccagt cataaggtct ttttctgatt
     4561 ttagtttaaa ttcatttgcc attgctagtg cttcttgtgc tttttcagtt attgttttag
     4621 ggtaatactc atctttcatt tggtcaaagt ttggaataga atggtgaaca tggataagtt
     4681 taattatatc atctttaact ctgtatacca tagtaaagcg agtatccatt tctacataga
     4741 tatctttttc taggtaatct ttttctatta cccaaagagt accatatact ataaaagttt
     4801 catctgtgac tttggtacat gaataccact catctataat ttcaaagtct atttgatttg
     4861 attctacttg gtctttttca agacctataa taaattcttc taatgtttcg taaaattcat
     4921 gttttcctgt tccaattaat gtaaggttgt catctaacaa agaaataata aatttaatgc
     4981 cttcttcttc tggatttaaa atataaatat tccaaatttt ttttgataaa tctcttaatc
     5041 ttttttgtgt tgtattattt ttcatagatt acccccttaa gatttacagt ttatatatta
     5101 atgtattata agttgaaccc ttattacaat cttattataa tatagaactg aagaaattac
     5161 atcctgtata ggtaaaaagt atatctttta tgataaaaag attaaagaaa aataacaagt
     5221 tttaatatgc atattatatt cttaatttat aaatgaatat tataaatgaa atttaaattc
     5281 aaccctgagg aaataaacta aatatattag tatatgaatc tatacttgaa cataaataac
     5341 ctttatgata tgatgttgat aataatatta tgagaggagt gccttgatgt cggagttgta
     5401 taatggtaac caacaattaa atagttggag acacatatat tctgtataat cagaaatttt
     5461 aagtgtattt ttaatacatt taatttgtgt gtcttattct gcctttatag ataactgaca
     5521 tatgacaaca aatagtattt aaaatatata ttactttgtt aaattatggt gtagtaggtt
     5581 ttaatctgct acacttttat taattttgta tagtattatt ttgataacta tgttgaaata
     5641 gagagcatta acttttctag tccacaggag ttttctgttt ttgtgctgtt aaatataagc
     5701 atagaaaaag gcagatatat acctgtggat ttttttatat aaattatgaa atatggaggc
     5761 gattaaaatg agcataattg aacttaatga actgactaaa tcttatagaa gatttgagaa
     5821 aaaggcagga tttataggga gtgtaaagtc tttatttaat aggaaatata cagaaaaaac
     5881 agctatatca tcatttgatt taaagataga agaaggagaa tttgttggat taataggtca
     5941 aaatggagct ggaaaaacta ctcttattaa gatgttaact ggtattattg caccatcttc
     6001 tggagaaata tctgttatgg gttattaccc taataaatta gaaaataatt ttaaaaaaca
     6061 atatgctatt gttatgggtc aaaaaagtca actattcttt gaattgacac cagcagatac
     6121 gtttctttta tttaaagaat tatatgatat atctaatgaa gaatataaaa aaaatttgga
     6181 ttactttatc gaattatttg aggtatctga atatttaaat gttcaagtta ggacactttc
     6241 cctaggagaa agaatgaaaa tggaactaat agtagccctt ctacataatc ccaaaatatt
     6301 atttttggat gaaccaacta taggtttaga tgctgtagcc caaaaacaaa ttaggtattt
     6361 tctaaaagag gtaaatgaaa caaaaggaac tactattatt cttacttctc attatatgga
     6421 agatattaag agtctctgta gtagaagtat tgttgtagaa aatagccgta aaatatatga
     6481 tggctctaca gaggaactat ttaatcattt tcaaaaatat aaaaaaataa cagttagttt
     6541 ttccaatgaa actaacattg tattttcaaa agattgtaaa gttgttgaac aaacaaatca
     6601 taagacctca gtagttgtgc caaaagaaag aggaaatgaa acgattaaat ggatttttga
     6661 tagatatgat gttaaagatg taatgattga agaagaagat atagttaata ttgtagagag
     6721 aatatataag aaaggaagtg atgagtatta tgagcaacta taatgaagca agttcaaatt
     6781 cttcaatgtt ttataaattt aaacagagta ttagaaggta ttatgaagta gccaaaatta
     6841 actttaaagt gcaacttatc tggagatttg atgtggtagt taatatgatg tttacgattg
     6901 taaaaatttt atttgcttat attttatgga aagcaatttt tggcgaaaat aaaatagtct
     6961 ctggttttac atttaactct atgttgtctt actatataat aagctcattt ttatcaggta
     7021 ttgatatgtc gaagagtacc agtgaagaga tatgtaatcg agtaaaatta ggcacatttt
     7081 caaagtacat gattattcca attaatgttc aaaattattt tattgctaaa aattttggaa
     7141 tgacatgttt ttattttgta tttaatatga tttctgcatt tgtatggatt tttatattta
     7201 aaataaattt tgcctttaca tcaaatccaa ttcaaatatt aatttcaatt tttatgattt
     7261 ttctaggact tatttttatg atgaaactcc acttctttat tgggattttg tcatttaaat
     7321 ttcttgagat tagttttttt agaatgataa tagaaaatat tgttgaattt attacaggaa
     7381 caatgatacc acttgtgctt ttaccagaaa ctatagtaaa agttatgaag gtatttccat
     7441 tttattatac aacatatttg ccatctatgt tactaatagg tagaaacagc aatgaagcaa
     7501 tacaaggatt aattatttta gcattatggg ttttattctt ttctgtagta aataaagtta
     7561 tgtataaaag aatgagaaca ttatttgatg gggtgggtat atgatgaaaa aaaatttgaa
     7621 aataatattt atgttattaa aaatgaaatt atcaaagatg atggtattta gatttgattt
     7681 ttttggagca ttttttgtag atagttcatt atttatatta caacttctaa tgtttaatag
     7741 tatatattct catgtagact caataggggg atggcaacaa ggagagatgt taatatttat
     7801 aggaactttc tcattaataa atgcgataaa tatgacgatt ttcttttttg gtatttatga
     7861 tattccacga aaaattcagg aaggagaatt agactactat attacaaaac caatagaccc
     7921 actatttagg ttaacatttg aaaatattaa tattggttca tcaccattaa ttttagcaag
     7981 tatacttatt gttttatatg gaatctctga attaaactca gagataactt tagtcataat
     8041 aataatatat attttcatgg tgctattgat gacattactt ttttatgatg tatgtattat
     8101 acttagaaca gtttcatttt ttgttatatc atcttctgcg ataatgagat tagaagataa
     8161 tttattgcca atgaatatga aaattcctgg tgtaatatat gaaggtgttt ttaaagtatt
     8221 attctattta atcttaccat atggaattat gtccacaatt ccaactcaaa ttttggctgg
     8281 aacaataact attaatggat taatatattc aattttaatt gtattttcgt ttacattgtt
     8341 tatgtggaga ttttggaagt taggtctgag acattataaa agtgcaagta gttaataaat
     8401 tggcgaaaat agacaatata attatatata aatacgtttc acatgacact agactatgta
     8461 aacgagtatt tacactaaag gcattgaaag ctataaaatg attaaagtaa ggatttaaaa
     8521 gttactttag tataaatata attgtgtaaa aaagcaagat taattaaaac tatatccaaa
     8581 taattagata taatttttat gcagaaagga ggtctgtaaa tgtctaattg tttggaatat
     8641 attttaaaaa taattaatgt acagaaaaat aaacaaaatg agtgatgtat attattcaga
     8701 aaatcatgaa caagataatc tttatgagca agataattta ctggcacaaa gatttaattt
     8761 atttactcta ttgaagtttg catttcctac tatttttatg atgattttta tgggattgta
     8821 tacaatcgtc gatactatat ttgtatcaag atttattaat acaaatgctc tatcagcaat
     8881 taatattgta tgtccaatta ttaatcttat agttggaatt ggaactatgc ttgccactgg
     8941 aggaagtgct attgtagcac gtaagatggg aaatggtgat attgagggag caaataaagc
     9001 tttctcttta atcgtattat caggagttgt aattggggta ataattactg gtataggtat
     9061 tgtatttatg gattatatca ttatatggtt aggagcaagt gaagttttaa ttaaatattg
     9121 tcattcatat atttttatta tcttgatttt tgcttctgcg aatatactac aagtattatt
     9181 tcaatgttta tttgtaacag ctggaaagcc tcaatttgga ttatggctag tgttaggtgc
     9241 agggtgtgct aatataattc ttgactatgt gtttattgtt ctgttacaaa tgggtattag
     9301 tggagcagca cttgcaactg gagtaggata cctaattcct accatttcag gtatggtttt
     9361 cttttcaaaa aataaagggg tacttaaatt ttgtataccc agactagata aaagaatatt
     9421 atttgaagct tgttttaatg gttcatctga aatggtgggt caactatcaa cagcagttac
     9481 tactttttta tttaatgcta tgatgatgaa attaattagt gaagatggag ttgcagctat
     9541 tacaataatt atttattctc aatttctttt aacttcgctt tatataggtt tctcaatggg
     9601 tgttgcacca ataataagtt ataattatgg aagtgaaaat aatacacagt taaatcatgt
     9661 ttttaagatt tgtatattat ttatttcagt ggcatcttta ttggtattta tcttttctat
     9721 atcatgttca ccaatcattg tagagatttt ttctcaaaga ggaagccgag tttatgaaat
     9781 tgcaaggtct ggattttata tattttcttt tagttttatt ttttgtggat ataatatttt
     9841 tacctcatct atgtttacag cattgtcaaa tggcaaggtg tctgcaatat tatcttttct
     9901 acgtacattt ggatttataa ctataggttt attgatattg ccaaatatgt taaatataaa
     9961 tgggatatgg ttagcagtac caagtgctga aattttaact ttatttttaa ccgttttttg
    10021 tatatgtaag cataagaata gatataaata tatgtagata ttaaataaat agtttaggta
    10081 gatattttat gttttaataa gtaattcaat tagtggtaga tagatactat tgaaaattaa
    10141 agttataatt gatataaaga attaataaaa ataacatata aaagtttaaa aggggagttg
    10201 aacttaaagt ttgattagaa aaagtcgatt ttaagacaac tccttttatt ttactattgt
    10261 attaatctgt atctgtaagt gtattagtct aatattataa actttggatg aaattaattt
    10321 tctataggtt ttaatgtatc atatacttta ttttaaggaa acgtatctta attttcatag
    10381 tgattgaatt tgtatattag agatgttact attagtaatg taaaagcaag agtagtgaat
    10441 ataaaaaaac aagatagtga tactgcaaga ttagaatata ataaattttt atggagagat
    10501 ttcaatatgg atacatattt taataaaaaa atattacttg ttgatgatga aaaagatata
    10561 gtagatttaa ttgaagaagt acttataaat gatggtttta aaaacattat taaagcttat
    10621 aatggtttag atgctatttc gctgtgtaag atagcatgtc cagatgttgt cattcttgat
    10681 ataatgttac cagatataga tggcatagaa gtgtgtaaaa aaattcgaga gttttcatac
    10741 tgttctattc tttttctctc atcaaaaaat gatgatattg acaaaatact aggtcttagt
    10801 agtgggggag atgactatat aacaaagcca ttcagtccaa gagaaattgt ttttcgagtt
    10861 aaagcgcaat tacgtagaca acagtatcaa agtattgtac catcagatag ttcagttatt
    10921 aagataggag atattactat tgatatagaa ggaaaccgtg tatataaaga tagaaaagag
    10981 attgagttaa ctggaaggga gtatcatctt ttaagctaca tggctcaaaa tgtaaataaa
    11041 attataggaa aagaaagact ctatgaacaa gtatggggag tatacagtag tatttgtgac
    11101 aatacaatta tggtgcatat acgacatata agagaaaaaa ttgaagataa cccctcaaat
    11161 cctaaaatac ttataacagt taaaggtcta ggatataaac ttgtgaatag aatcgattag
    11221 aatagatctt agtatcaatg aaggtgtctc aaattcaatt tgttagttaa tgagacattc
    11281 ttttttgtgt gaaatcaaat gtatttttgt tatatcaata atgaagaaga ggcttatctc
    11341 tattttgaga tagcctcatt aaatttatac cgtggtaatg cactttatgc tctaataaat
    11401 atttattttt tagtttactt gcagtgaaac attaactagg agattaaaat ttgcattgaa
    11461 atgctaatta aacaatatcg catattgtat ataaacctac actaattgtt tttctatatt
    11521 aaatactttg tcaatccagc ctttataaaa tttttcttca tgggaaacta gaattatact
    11581 tcctctaaac tgtttcaaag cattttgtaa agcatccttt gtttctgcat caaagtggtt
    11641 agttggctca tctaaaatca aaaaattaca agggaataac atcatacaac ataattttac
    11701 cttagattgt tcaccaccac ttaatgtaga tacactcctt gaaacatgtt cttcctttac
    11761 gccacatctg gctagatgac gtctaatctc ttttgtattc aatttcggat atttatctgc
    11821 tactatttga agcggtgttt tagtaggatt ttcccatttt aaatcttgct cataataagc
    11881 aagtttcact tgttcagaaa actggaaatc tccagaaata caaggaatat ctttaactaa
    11941 tgtttttaac aaagtagatt taccaattcc attaaaaccc gttataacta ttttttgacc
    12001 cccatcaaca gagaaattta attttggaag caaagaatag tagtagccaa cttcaagatt
    12061 tgttactgta agagcatttt gagcagatat ttcaatttct gaaaatttta tatttggttt
    12121 tcctgtaaaa ccaggaggag ctattctttc tattttatcc aattgttttt gtcttcctct
    12181 tgcaattttt gaatttactc cagctttgtt tttacgtata aatgcctctt ctttttctat
    12241 tttcttttgt tgtgattgat atcttctaat ataatcctct cttaaatgta atttttgttt
    12301 aaggaactct gaatattttc catgatattt cttaatcata ccaaattcta tatcaagaat
    12361 acctgtagaa attttctcta aaaagtcaaa atcgtgagaa actacaataa atgctccact
    12421 aaatgtattt aaataatttg agagccattc aacgtgttct ttatctaaaa agtttgttgg
    12481 ttcgtctaat aataaaacct catggttaga aagtagcaat tttgctaaaa ttacctttgc
    12541 cctttgccca ccgctaagct ccccaacaac acgattcatt cctattgaat ctaatccaag
    12601 accatttgct attttattta cttcattatc tatagaatag aaattatttg ctattaattg
    12661 ttcctgataa tctgatgctt tgattagatg ttgctcattt ccagatatag cactttcttg
    12721 atagagtaaa ttcatctcct tttctatttt ataaagttct tcaaatgcag tgtgtaaata
    12781 gagtgatatt gttgtatctc tattgatttc tgcatattgg tctaaatggc ctatattgat
    12841 attaggttgc cacttgattt ctcctgaatc tggaataact tctcccaata aaattttaat
    12901 taatgtactt ttgcctgtac cattttggcc aactacacct atatgttcac ctttgaataa
    12961 atcaaagtta gctttctcat acagtgattt atccataaaa cattgtgata aattagttac
    13021 ttttaataaa ctcataattc aaactccttt tcttttatta aaaaatttat ggaaataaaa
    13081 aaagcagagg aatgacatta taaaatgccg acctctgctt ttggaaaaac aaaaggtcat
    13141 agacaagcat tgttgtctat gaccagaaaa tctatattat ctatcggcaa ataggtaatt
    13201 ttaaataagc atagcccaaa aactacacta aacctatatt tgccgatgaa gatagaaagc
    13261 tttaagcttt cagattaacc tctacacaac gcttcatcgt atacatttgt gtagagttaa
    13321 gttttctaac aagtttaact aattctatat aaatcataat tattcacatc ctaatcttag
    13381 tattaattct agtttatcta tatttaaatt gtacttttgt actttgtata ttgccttatt
    13441 tacttaaaat atattttatt aattatagct taaatcatgt tatcataata cttagttata
    13501 gtcaataaga aagttatatt tgctacataa cctattaatt tagtaaatct atcattacaa
    13561 agttatttga agactcacca ccaataaaat tttataatct attaaaaaat taagattacg
    13621 agatttataa ttttgataca ggtatactat ctttattaaa tattgcaaca tgtagaagta
    13681 gatggataat attattaaat tttcaatgat atgctaatgt acatatataa ttattagaaa
    13741 taaaaatata atagtatgaa agtaactgat ataataacta aatgattaaa catatagtaa
    13801 aagtacttca taaattatgg agtatctttt attgtcgcaa aaataagcaa tatattatac
    13861 aaaataaaaa cattaattca acttaaataa ttttaagtgt aatatgtatt ttgattaaat
    13921 aagctaaaat tctaagaaaa aataatatat ttgtattaag ttttattgaa ataattaaaa
    13981 ttatttactt aatgtaaatt gttagcttaa attatattaa ataattaagg cggtgagtgg
    14041 gtctggatga gatagattta aaaataatta attctctaag aaagaatggt aggtttcaac
    14101 ttctgaaata agcagaaaaa ttaatttatc aataccagca gtctcagagc atataaggaa
    14161 aattgagcaa ttaaatttaa ttcaaaagta cactattcaa attaatcagg aaaaaataaa
    14221 atataagctt ttggcttttg tatttatcaa tacggattta tctaaaagta tggaagaatt
    14281 taagaaaact atattggaat atgatactgc attagaatgt catcatattt aaatctaata
    14341 agggagttta atttaaaaga aaaaataatc gcttttattt gtgtgtcagc attgctgatt
    14401 ggaaaaagtt gtgttttaaa aaatcgtaaa gctactacat atcatttgaa aaatagtaaa
    14461 agacaaagac agttaagtga gtttgatgta aatgtagtta atgaaccaac tgttgtaaat
    14521 caaaacatta taacctcata ttatacagaa acagctccac atgtagcttt taagctttta
    14581 gaaatgttga tatctaaaaa acaactggat gaagttaaat tagctatggg atttaaactg
    14641 taatagttta gagcttttcc aagtaatcag aaccccagtc gcacatggaa tctaaaattg
    14701 gaataatact ctttccaaaa tcagatagtg agtattctac ttttggagga acaacaggat
    14761 aaaccactct tatgattaaa ccatctgatt ctaattcacg taattgttga gttaacattt
    14821 taggtgttgc cttagggatt aattttttta attcattgaa cctcaatata gtgtctttaa
    14881 gatgccaaag tataagtgtc ttatatttac caccaatcaa tgcaagtgtt gcttctattg
    14941 ggcaactgta attatctaaa cattttttca tttttggact cctttattta ttttaaagta
    15001 tctttttagg tactatatta caaaaaagtg cattattgta atatattacc atagtgctaa
    15061 aattatagca taggttaaat aaatcatcaa cttagtctaa taaaacaata ggaggtaaat
    15121 aaatgaattt cgttgaactt gcaaaaaaaa gatattcttg ccgtaattat caagatagaa
    15181 aggttgaaaa agaaaaactt gataaggttt tagatgttgc aagaatagct ccaactggtg
    15241 gtaatcgtca acctcaaaga ttgattgtta ttcaggaaaa agagggtcta aataaacttt
    15301 ccaaggctgc caatatatat gatgctccat tagcaattct tgtgtgtgga gataaagaga
    15361 aggtttggac aagacctttt gatgggaagc aacttacaga tattgataca agtatagtta
    15421 cagaccatat gatgctacaa gcaactgaac ttggattggc aagtgtttgg gtatgctatt
    15481 ttaatccaga tataattaga gaagagttta gtttgccaga taatctagaa ccaataaata
    15541 ttttattgat gggatatgaa tctaaggtag cagatagccc agatagacat gaaaaaactc
    15601 gtgttccttt aagtgaaatt gtttcttatg aaattcttta attataaaat taagttattc
    15661 ataggttaaa tgtaacaaag tgtttattat aaataaaact caaaataaat aatatcctta
    15721 gataaataat atctatataa ttaattttag tgaagtatat ctagtttaac tagaatatat
    15781 acttcactaa tttatttgtt caaaatattt gtgaggattt taatatatta actaaaatat
    15841 atcaattgaa ttaattttta aatctggaaa agcacttgaa atatagtatt ttatgtcatt
    15901 taaatggtct tgaatttgtt tttctgatat tttatcaggt ccatatacaa aatacataat
    15961 atttttagta tcttttgtta tagctgttcg attatcaggt ccattaagaa tgctagataa
    16021 tataccaata ttatccgata aaaataaatc atcttttgtc aaaatctgtt ctttagcacc
    16081 tataccataa aaatgttcac ttccatttgc taagtctatt ttcaaaggta attctatttt
    16141 atcaaggtca tgacctgctg ttagaagaag atttttcaat tctgctaaaa acattgactc
    16201 aattgttaca cctacatctg ggataccttt tgattttaat agaatagatt ctaattgaag
    16261 caatacaggg taagttttct tgaatttttt gtaatacttt atataacttg aaacaggctc
    16321 tgttttaatt ttttctttac gattgtattc tggatgtttt tctataatat ttttaattgt
    16381 agagttttta agaattaaaa aattttcttt attaggtttt gaatgtgtaa tatttataac
    16441 cataactcca aatttaatat tgggataaat ttctttcact ttatgtgata tttctaacat
    16501 aatatagact cctttttatt ttttgtttta tgatataatc ttagcaagtt aaaagtagat
    16561 tttgtagttc catttttata gtaattttat agaaccactt ttggaggtat tagaatgtgg
    16621 attacgatta atagaaatag taatatatca ttatcaagac aaatttattg tgagataaaa
    16681 gatatgattc tgaatggaag cttaaagttt gagcaaaaat tgccatcatc taggacttta
    16741 tcaaaagaac tatctgtatc tcgtaataca gttttagatg catataatca attaattgca
    16801 gaaggatatt tggaaacaag gaatggatat ggaacaattg tagctaaagg aatatcaggg
    16861 cataaagttg attttaagtt aaattatatt tttagagatg aaaaagattt acatactaat
    16921 aaacaaaaaa attatataga ttttcgttca ggagttccag aattaaaact atttccacgc
    16981 aaagaacttt caaagctata taaacaaata ctgtatgatt tatctgattc ggatttcaga
    17041 tatggaagta cagctggtgt atgggtttta agagcggaaa tatctaagta tttatttcgt
    17101 actagaggaa ttagatgtaa tcccaaaaac attatgattg tttctggttc aacacaaggt
    17161 ttatctttaa tttcacagct tttatacaaa agtaataaaa aggttgctgt agaagaccct
    17221 attcataaag gcttattaaa ggttatttca tctattggat attcagttgt tggagttaaa
    17281 gtagatgata agggaataaa taccaactta ataaaatcaa taaaggggat ttcttttgtt
    17341 tatactacac cttctcatca atacccatta ggagggattt tacctattca aagaagactg
    17401 gagcttatta aatatgcaac ggagaatggt tactatataa ttgaagatga ttatgatagt
    17461 gaatttagat atgagggtca accaattagt tctctttatg aacttaaccc aaataaagta
    17521 atttacatag gttcttttag taaaatatta tctccagcca ttcgtgttgg atttatatta
    17581 ttaccagaca atattttaga aagctataaa accttaaaaa tgtactctga tgttcataca
    17641 gaaacaatat tacaacatgt attagctgaa tttattggaa atggaggttt agagaagtac
    17701 atatttagaa tgaagaagtt gtataataga aaacgtaagt acctcataaa agttttaaac
    17761 acggaattta aaaatgaatt taaaataaaa ggtgaagcag cagggcttca tatagttgct
    17821 catttctata atgttaaatt tacggaaagt ttaattcata aaatttttga agcaggcata
    17881 aaagtttatc ctatacaaca atatgcaatt gagaaaatat tagaatatga ccatgaaatt
    17941 atactaggtt atgctcatct tagttttgat gaaattgaaa aaggtgtaaa aatactcagc
    18001 aaaataatac atataaatca aggtacatag ataaaacata tagcaatcta catataatag
    18061 atgcaatgaa gtttaaactt taaaagaaat atatattaat atttttgtat aaaaatacct
    18121 gatattgtgt tatcgatact ttgttgctat acactaaatt aagatagcaa tttatattaa
    18181 gaaaaaactt tttgcatagg ttataatact taaaaggaga ggatagagtt gaaaacaaat
    18241 acagatgtta ttatggagat aattgattat atagaagaaa atcttacaga aaatctaaat
    18301 ttagataaaa tagctgatat tgctaattat tcaaaatacc atttacacag aatgtttaca
    18361 aacattgtag gatgtactat tcatcaatac atccagagga ggagactcac tgaagcagct
    18421 agacagctta tatttacaga caaatatatt atagacatag ctattacagc aggatattct
    18481 acacatcaat cttttacact agcatttaga aatttatata aagaatctcc acaagtatat
    18541 agaaataaag atgaatttta tcctattcaa cagagatttg ttttaaatct aaatcatcag
    18601 attcaaagcg aaaaatatat ttcttatgca aatatgaatt gtaaacaaag aatagatgaa
    18661 attaattctt atcttaactt taatattaat tgtgaggtga aagtagcatg agtttagtac
    18721 cacatgtaat tgaacaaaca agtcaagggg aaaggtctta tgatatatat tcaaaattat
    18781 taaaagatag aattatattt ataggagaag aaattaatga tgctattgca agtttagtag
    18841 tagctcaatt actgttttta gaatcagaag acccagattc agatataatt atatatataa
    18901 atagccctgg tggctctgtt acagcagggt ttgctatata tgatactatg aattatgtca
    18961 gatgtgatgt atcgactatt tgtattggta tggctgcaag tatgagtgct tttttacttg
    19021 caggaggaac acatggaaaa agatatgctt tacctaatgc agagattatg attcaccagc
    19081 caatgggagg agctaaaggt caagcaacag atgtaaaaat agctgtagat aatatattga
    19141 aaataaaaga acgattagat agaatttttt ctgaaaatac aggtaaatca atagaagata
    19201 ttagaagaga tacagataga gataatttta tgactgcact agaagctaaa gagtatggac
    19261 tgattgatta tataatgaat aggaatgaat aaagtatcat attaaaatat ctgttttata
    19321 aatatatata gaatattaaa taagagtgtc tcaaagtgaa ctttttagtt tatgggatgc
    19381 tcttttttgt atgaaattaa atatagtttt attatttcaa taactagtat caaatttcaa
    19441 gtttaataaa ttctaccata aaaatacata aaagataagg ttttttagta actttctgtc
    19501 gtcaaaagtt tttataaatt taataacaaa acaagagaaa ataccatata ttacttatgt
    19561 ctgcaatata tagaagacaa aggagttgta cattatgatt acaataagtt tgtgcatgat
    19621 tgttaaaaat gaggaagaag ttataggcag atgcttagaa tgtgtaaagg atatagtaga
    19681 cgaaattata attgtagata ctggttctac agattccaca aaagaaatag tctcaaaata
    19741 tacagacatg gtgtacgatt tcgaatggat agatgatttc tctgctgcaa gaaatttttc
    19801 attttctaaa gcttctaaag actatacgat gtggcttgat gcagatgaca taattttaga
    19861 agctgataga gaaaaattat taaagattaa agaaagcttg gatacttcca ttgatattgt
    19921 tatggcaaaa tacaatgtat cctttgatga aaatggtagc ccaactctct cgtattatag
    19981 agaacgattg tttaaaagaa gttctaatca taaatgggtt gaccctattc atgaagtagt
    20041 acctctttct ggagaggtat tttattctga cattgcaata tctcataaaa agcttcatag
    20101 acaagaccct ctaagaaatc ttagaatttt tgaaaaaatg atttcagaag gaaaagtatt
    20161 ggagccaaga caccaatttt attactctag agagttgtac tataatgcta agtataaaga
    20221 agcaatagaa ggttttacta agtttttaga ctcatcaaga ggatggattg aggattgtat
    20281 cagtgcttgc agagatttag ccacatgtta ttacttaatt aatgatgaaa agagtgcctt
    20341 atattctttg tttagaagtt ttgaatttga tgagccaaga gcagaaattt gttgtgacat
    20401 aggaaagcat atgtttgata gacaaaaata taaagaagct attttttggt ataaggtagc
    20461 actaacaaga gataagaatg atactaatgg aggttttaag tcaaatgatt gttatggata
    20521 tataccatat atacaattaa gtgtctgtta tgacaggcta ggagaatcaa ataaagctat
    20581 ttattatcat gaaaaaacaa aagaaatcaa acctaatgat agtgctgttt tgcacaatga
    20641 aaattatttt aataaattta aaaatagttg ataggtcaca ataatactaa atttgacata
    20701 ttatatatag agcttagaag ctcaaatata tcaggaggtg cttttaataa tgagtagaaa
    20761 taaatatttt ggaccatttg atgataatga ttacaacaat ggctatgata aatacgatga
    20821 ttgtaacagt ggtcgtgatg attataatag ctgtgattgt catcattgct gtccgccatc
    20881 atgtgtaggt ccaacaggtc caatgggtcc aagaggtaga acaggcccaa caggcccaac
    20941 gggtccaaca ggaccaggag taggtgcaac gggtccaaca ggtccaacag gcccaacagg
    21001 tccaacagga aatacaggag gaactggaag aggaggccca acaggtccaa caggtccgac
    21061 gggtccaaca ggtccaacag gcccaatagg atttggaata acaggtccaa caggcgcaac
    21121 tggagcaaca ggtgcaaccg gagcagatgg agtaacaggc ccaacaggcc caaccggagc
    21181 aaccggagca aatggaataa caggtccaac aggtgcaacc ggagcaacag gatttggagt
    21241 gacaggtcca acaggcccaa ccggagcaac aggagtagga gtaacaggag caa
//
LOCUS       DAMJVM010000238        15611 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.guided.085, whole genome shotgun sequence.
ACCESSION   DAMJVM010000238 DAMJVM010000000
VERSION     DAMJVM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15611
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.guided.085"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            <1..153
                     /locus_tag="PC523_003605"
     CDS             <1..153
                     /locus_tag="PC523_003605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2477054.1"
                     /translation="GNSGGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGA
                     NIGKTE"
     gene            261..998
                     /locus_tag="PC523_003606"
     CDS             261..998
                     /locus_tag="PC523_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2477055.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTRLNEENFSIKLFKADVSNREDVENMVDYCIKEFGGLDVLVNNAGISQDKLFTDITD
                     VDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDILVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            1096..1980
                     /gene="rsgA"
                     /locus_tag="PC523_003607"
     CDS             1096..1980
                     /gene="rsgA"
                     /locus_tag="PC523_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF2477056.1"
                     /translation="MLEGKIIKGISGFYYVDTHNGIYECKARGIFRKHKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKKIYELSGYKVIPVSNITKLNIEKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVANGEISKERY
                     ESYIQLLNEIRQNNSRRY"
     gene            1985..2656
                     /locus_tag="PC523_003608"
     CDS             1985..2656
                     /locus_tag="PC523_003608"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF2477057.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPIDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVDKVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            2665..3306
                     /locus_tag="PC523_003609"
     CDS             2665..3306
                     /locus_tag="PC523_003609"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDF2477058.1"
                     /translation="MKICIVLNGEIEDYEVTRGIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     KGDAKGVTLTGLEYPLDNYYMRYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            3437..4066
                     /locus_tag="PC523_003610"
     CDS             3437..4066
                     /locus_tag="PC523_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2477059.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSKHKEIDLSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERNNLLNECSLKECEYLSENGAKLFDDIENTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFVDFECPGNTGLHKGENNKLIIERNKLKNPI
                     YIGDTQGDANSAKFAGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            4121..4777
                     /locus_tag="PC523_003611"
     CDS             4121..4777
                     /locus_tag="PC523_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2477060.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVIIKRRDLMEKIADVITKKQQEILVEMEKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            5172..5894
                     /locus_tag="PC523_003612"
     CDS             5172..5894
                     /locus_tag="PC523_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012422616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2477061.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIIDIMMPKMDGITLTMKLRENHNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVENKEQKVDDDDGVFAIGGLELNENTKEVSVDG
                     KHIKATPIEFKILSLLMRNAGRVFSADEIYERVWNDNAVNTDTVMVHVRNIREKIEVD
                     PKNPKYLKVVWGVGYKIEKIQR"
     gene            5863..8196
                     /locus_tag="PC523_003613"
     CDS             5863..8196
                     /locus_tag="PC523_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2477062.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQAGAKGGA
                     KNPFEQESFVDNMYKGSYVLYHNMKEEQEGKMIQPSKLFISKETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNQNFENWESFIKESGKNLKYYLVDKESNFEFFNDNKELGALD
                     TTKNYASKEDSDENKSKNTEMSDEERQKQIKDIKENYRFYIVMNFDKDGKVNVLDSYG
                     VNQKVISQMLLSKAREDLMNTTLGGNPISYKLSPIKNVTLVYAVPKVISKGDGGEYSY
                     GNIYNNDSISSYINNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIILAILQGIVDLYPTSIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIKEYFIKNTVTGMFFVWLIKSGKVVINQIRITDLK
                     EKPNKTIAIILGINLLIIVIMCSFWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLEVNMERDLGFFNPIKDELGNIQAGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKVNSGD
                     VHLNIMNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVSIMLKNMSATEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(8475..11153)
                     /gene="mngB"
                     /locus_tag="PC523_003614"
     CDS             complement(8475..11153)
                     /gene="mngB"
                     /locus_tag="PC523_003614"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDF2477063.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRKKLAIGPWYTQTDQLVISGESIVR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDTIGKLKVKSSTNNVYFPNG
                     FDQAPIRKNLPDILKKANKIDTENEYEISDIENYVDSVRNERNEFTELKGEFVNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLILKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDFHMRLISTQIKNNNEEITLTVFNGL
                     PEARNEVVEFDTYIPGQDFVIKNKNGEILNYIIKEKEDITDYILTQTIKLNPSKKIYI
                     PKTVYRAKIALSVKNIPPMGYTQLIVELDTKNKCEIKTSSCKKIENKYYKITANKNGS
                     VEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNVQEE
                     MTLKYSLTIPASLEERTIGKVSVKMPVTMKITLEPKEQIIKFNINIKNDQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTALEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQKGVALITGCVREYQIVGANLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFNFGLYIYNNKFDKSNIANTAKRFLTPIQIYEYADFLNGRLIFVFNDEKQ
                     IYENEYSFMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDSKGSLNINKNINAAYN
                     AMLDEIYDNTNKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(11227..12333)
                     /locus_tag="PC523_003615"
     CDS             complement(11227..12333)
                     /locus_tag="PC523_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2477064.1"
                     /translation="MSKKNNSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAM
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPSMGAGFIGGL
                     IASNTNAGFLGAVTAGFFAGYICKFLKKKIKISGSAAGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGSMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSRFSKTLSETGKGNIVMGIIGITEGAI
                     PFTLKNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISVGSGWAYLVGAIF
                     GALVIAILSTILVDFNEDETAENTEFDEIELDIM"
     gene            complement(12448..12756)
                     /locus_tag="PC523_003616"
     CDS             complement(12448..12756)
                     /locus_tag="PC523_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2477065.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHKIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGVALKKPEAVLTKCIETIS
                     "
     gene            complement(12759..13211)
                     /locus_tag="PC523_003617"
     CDS             complement(12759..13211)
                     /locus_tag="PC523_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2477066.1"
                     /translation="MVLQEKNIFIDKDLKSKEEVLNFIADSAYILGITTNKDALLKDL
                     LKRESEFPTGIRDGFAIPHTKSANVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HENEGNIHLKTLSKLATCLMEDDFISEVKNASDASHLVKYITNKMKEE"
     gene            13446..15374
                     /locus_tag="PC523_003618"
     CDS             13446..15374
                     /locus_tag="PC523_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2477067.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSINE
                     TLEKNGAIIKIKKGEGYYIDILNLALYQHYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQKIVLSEINKFNINFLDFNLK
                     NFIIHLSITISRILGGYCLDNVLDVVLTDFQSNTAVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRADTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEISLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQNNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSRIYSNLLDNFFDKNLF
                     IVNPKIKEKDELIKLMCNKLTQSEIVIPSFTESVFYRESLSSTNIDDFLAIPHPMELS
                     SIKTKICISILNEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDSFLSIIKSII"
BASE COUNT         5797 a         1837 c         2429 g         5548 t
ORIGIN      
        1 gggaactcag gaggctcaaa ttcaggagac aatggaggga gctcaggcgg ttcaaactca
       61 ggagacaatg gagatagttc aggaggctca aactcaggag ataatggagg aacttcatca
      121 ggcactggtg caaatattgg taaaacagaa taaatattaa taaaataaga gctgtccttt
      181 tatagaaaat attttcttta aagggcagtt tgttttgtat aaaatattga attggttata
      241 atataaggag aaaagcattt atgaaaaaga aaacagtttt aattacagga ggagctagag
      301 gaattggcaa agctatgtct aaagcttttg caaaagaagg ttataatgta ttagtaaatt
      361 ttaataagtc agaaaatgaa gctaaagagt tgtatactag attaaatgaa gaaaattttt
      421 ctataaaact ttttaaggct gatgtttcaa atagagaaga tgtagagaat atggtagatt
      481 attgtataaa agaatttgga ggattggatg tacttgtaaa caatgctggt ataagtcaag
      541 acaagttgtt tacagatata actgatgtag actgggataa tatgatgaat attaatttaa
      601 aaggaagttt ttattgttca caagttgctt taaaatacat gatatcagaa aaaaaaggaa
      661 atataattaa tatatcttca atctggggta tatcaggtgc ttcttgtgaa gtgcattatt
      721 ctataacaaa agcaggtata ataggaatga caaaagcatt agctaaagaa gtaggaccat
      781 caaatattag agtaaatagc atagctccag gtgtcattaa tactgatatg ttatctggat
      841 ataatgagga agatatagat attcttgtgg aagaaactcc tctaatgagg ctaggaactc
      901 cagaagatat agcaaattgt gctattttct tggcatcaga caaatcaaat tttataactg
      961 gtcaagtaat tagtccaaac ggaggatttg ttatttaaaa aaaatgtggt attatataaa
     1021 aataggaaag aataacacga tatactactc ataaacagta tgtgtttata taaattttat
     1081 aaagggaggc atttgatgct agaaggaaaa attataaagg ggattagtgg attttattat
     1141 gtagatacgc ataatggtat ttatgaatgt aaggctagag gaatatttag aaaacataaa
     1201 ataacacctc tggttggtga tagagtcaaa ataagcattg ttgatgagga tgaaaaaaaa
     1261 ggtatcttag aagaaataga cagtagagat acagaactta taagacctcc tatagcaaat
     1321 gtagataagg cactaatagt attcgcaata aaaaatccta aaccaaactt atctctttta
     1381 gatagattta tagttttggc agaaaaagaa aatttagaaa ctgtaataat ccttacaaaa
     1441 gcagatttag atgataatga tacactagaa actgttaaaa aaatatatga acttagtgga
     1501 tacaaagtta ttcctgtaag caatattact aaattaaata tagagaaagt aaaagaagag
     1561 ttaaaagaaa atgtagtagt gtttgcagga ccatctggag ttggaaaatc aagtttacta
     1621 aatgaaatag atgaaaactt taaacttcaa acaggagtgg taagtgataa aataaaaagg
     1681 ggaaaacata ctactcgtca tgctgaactt ttaaaattgg aatttggtgg aatggttgca
     1741 gatacaccag gctttagttc tcttgcactt gaagatatag aggaagttga acttaaagat
     1801 tattttatag aatttgataa atttaatgat tgtaagtttg gttcaaaatg tattcatgaa
     1861 aatgaaccta attgtgctat aaaagaagct gttgcaaatg gagaaatatc taaagaaaga
     1921 tatgagagtt atatacagct actaaatgaa ataagacaaa ataatagtag gaggtattag
     1981 aaacatgatt aaattagcac catcaatatt atcagcagat tttgcaaaat tattagagga
     2041 tgtaagaaag gtagagagtg caggatgtga atatcttcat atcgatgtaa tggatggaca
     2101 ctttgtacca aacattacat taggaccact tgtagtcaaa agtttgaaga aagaaaatat
     2161 aaatatggta tttgatgctc atcttatgat agaaaatcca gaccagtata ttgaagagtt
     2221 tgtaaaagca ggatgtgata taataactgt tcatcaagag gcttgtgttc atctacatag
     2281 aactatacaa aatataaaat cacatggaat aaaagcaggt gtagtgctta atccagctac
     2341 accaatagat actattaaac atgtactacc tgatttagat atggtattat taatgtctgt
     2401 taatcctgga tttggaggac aatcatttat accttgtgtt ttagataaga ttaaagagtt
     2461 aaaagctatt atagatagtc aaggattaaa tattgatata gaagttgatg gtggtataag
     2521 ccctaaaaat gtggataaag ttgtacaagc aggagctaat gtaatagtag caggttctgc
     2581 aatatttgga agtgatgaca ttcaagaaac agttaattta tttagaaaga atgcttctct
     2641 tgaagaatta gtttaaattt aagaatgaaa atctgtattg ttttaaatgg tgaaatagaa
     2701 gattacgaag ttacaagagg tattataatt aaagaatgtt atgactgcat aatatgtgca
     2761 gatggaggag caaatcacac ttataaaatg gaaataatgc cagattatat attaggagac
     2821 ttagattcag tagaagaaga aaagataaac ttctataaaa ataaaggtgt taaatttgaa
     2881 aaatttccat ctaaaaaaga tgagactgat acagagcttt gtcttttttt agctaagaca
     2941 cttaaagcaa atcatattga tttttttggt gctttaggag gtaggattga ccacactctt
     3001 gcaaatataa agctattata ttatttaaaa gaagatggta tatattctag gatactttct
     3061 gataaagaag agatgtatat agttgaaaat gaagaaattt cactgtatgg aaatccagga
     3121 gacactattt ctgtaatagc tataaaaggt gatgctaagg gtgtaacact taccggatta
     3181 gaatatcctc tcgataatta ttatatgaga tactctgtac ctattggaat atcaaatgtt
     3241 atgttgagta attcttgtaa gattaaagta gagcaaggat gtgtacttgt agttagaaat
     3301 ttataaaatt tggttttgac aattgatttt atattgtaga gcagatacaa tcaagtaaaa
     3361 tatatttatg attgtatctg ctttttagta tataattata aatagataat aaactttgac
     3421 ataaggggga ttttttgtgg tagatagtat tatttttgat ttagatggaa cgttgtggga
     3481 ttcaactgaa gaagtatgca aagtttggca ggatgtttta agtaaacata aagaaataga
     3541 tttaagtgtc acaaaagatt tatttagaag tcttatgggg ctttcttttg aggaaatagc
     3601 aaatcgttta tttccaaatt taagtgaaga agaaagaaat aatttattaa atgaatgttc
     3661 tttgaaagaa tgtgaatatt tatctgaaaa tggagctaaa ttatttgatg atatagaaaa
     3721 tactcttaaa ttactatcta aaaaatataa attatttata gtaagtaatt gtcaagctgg
     3781 atatatagaa tcttttttaa aagcacataa actagaacag tattttgtag attttgaatg
     3841 tccaggtaat actggtttgc ataaagggga aaataataaa cttataattg aaagaaataa
     3901 attaaaaaac ccaatctata ttggagatac tcaaggtgat gcaaatagtg ctaagtttgc
     3961 aggtatccct tttatatatg ctaaatatgg atttgggaat gtagatgtat atgattattt
     4021 tatagactca tttaaagatt tgttagaaca tgatatatta aaataggatg agtttaaaaa
     4081 ttatattaaa ataataaaaa agttatgggg ggatttaaaa atggaattac aggatactat
     4141 ttttaaaaga caaagtgtaa gaaagtttaa aaatcaagat gtgtcagatg aagatatttt
     4201 aaaaatgata aaagcagcag gtgcagctcc atcaggaaag aatattcaaa actggcattt
     4261 tgtcattata aaacgtcgtg acttaatgga aaagatagca gatgtgataa ctaaaaagca
     4321 acaagaaata cttgtagaaa tggaaaaagt atcagtagat aaggctaata gatttagaaa
     4381 atttgttaaa aactttacac tattttactt aaaagctcca gttttagtat tagtttttac
     4441 aaaggtatat aatccatcag gatattatga attagagctt atagatgcac ctaaggaaac
     4501 tatagataaa ttattcataa gaaatccagg tatgcagagt ttaggtgctg cgattgaaaa
     4561 ctttacatta tcagcaattg aacttgggta tggttcttgt tggttgacaa gtcaaaacta
     4621 cgcagcagat gaaatagaag ctgttttaga agcagaaact ggatttgaaa aaggagagta
     4681 ttttttaggt gcaatgttgg ctcttggagt gccagaggat aatttgaaga gcccatctaa
     4741 aaaaccagta gaagaaatat gtacatttat aaaataaaag acaataattt ttaagattca
     4801 aaatgagtta aaaataaata gttaaaaata aaaaggtagg ttttattgat attaagttta
     4861 tgtttcaaat cattgattta attaatgcaa taaaaactac cttttttgtg catattaatt
     4921 ttgaataact ccaaatcttt agaaaatctt tagaaaagta tatgtaatat cttaagtttt
     4981 taactttaat attaagtata taaaatgaaa aaactaaatt tatactagag ggaggtctct
     5041 aaggtgtaca ttaaataaaa ataactatta gaaagatata aatatataaa aaaatggaaa
     5101 aacttctatc atattgtata atcatatatg taaagaaaat ttataaatta attggtataa
     5161 aggagattaa aatgaattca tataatattt tggtggtaga agatgaaaag gaaatagcag
     5221 atgctataga gatataccta ttaaatcaag gatataatgt ttttaaagga tacaatggat
     5281 tagaaggact taaggttata gaaaatcaag aaatacattt agcaataatt gatataatga
     5341 tgcctaaaat ggatggaata acacttacaa tgaagcttag agaaaatcat aatttcccag
     5401 taataatgct atctgcaaaa tcagaagaag ttgataaaat aatggggctt aatatagggg
     5461 cagacgatta tgtaacaaaa ccttttaagc ctttagagct attggctaga gtaaactcac
     5521 aacttagaag atacactaag tatttaaata tggttgaaaa taaagagcaa aaagtagatg
     5581 atgatgatgg cgtgtttgca ataggtggat tagaattaaa tgaaaataca aaagaagtta
     5641 gtgttgatgg gaagcatata aaagcaactc ccatagaatt taaaatacta agcttactta
     5701 tgagaaatgc aggaagagtt ttttctgctg atgaaatata tgaaagagtt tggaatgata
     5761 atgctgtaaa tacagataca gttatggtac atgttaggaa tataagggag aaaatagaag
     5821 tagacccaaa aaatcctaaa tatttaaagg tggtgtgggg tgttggatac aaaattgaaa
     5881 aaattcaaag ataaatatat agttactatt atagttttta tacttatgtt atcagcatca
     5941 ttaggtatga ttagtcaata ttctactatt caagcagggg ctaaaggtgg agcaaagaat
     6001 ccatttgaac aggaaagttt tgtagataat atgtataaag gaagttatgt tttatatcat
     6061 aacatgaaag aagaacaaga aggtaagatg atacaaccct caaaactttt tataagtaaa
     6121 gaaactatga actatataaa aaatgtaagt aaagaaaaag atacttatga tggctcagag
     6181 acttattata cagatgatag tattaatgca gattttaatc aaaactttga aaattgggag
     6241 tcttttatta aagaatcagg aaaaaattta aaatattatt tagttgataa agaaagtaac
     6301 tttgaatttt ttaacgataa taaagaatta ggagcacttg atacaacaaa gaattatgct
     6361 tcaaaagaag attctgatga aaataaatca aaaaatacag aaatgagtga tgaggaaaga
     6421 caaaagcaga ttaaagatat aaaggaaaac tatagattct acattgtaat gaattttgat
     6481 aaagatggaa aagtaaatgt tttggattct tatggagtaa atcaaaaagt tattagtcaa
     6541 atgttattgt ctaaagcaag agaagattta atgaatacaa ctttaggtgg aaatccaatt
     6601 tcatataagc tatctccaat aaaaaacgtg acattagtat atgcagtacc taaggtaata
     6661 agtaaagggg atggtggaga gtatagttat ggaaatattt ataataatga tagtataagt
     6721 tcatatataa ataatgtaga aaataattct tactataaga tttctggaat gattttaaaa
     6781 attatattag ctatagttgt gattgtagct ttagcattcc catatagaaa atctaaagaa
     6841 ttactaggat ttgatacatt atcaagaata ccattagaga tattatttat tattcttgct
     6901 attttacaag gaattgtaga tttatatcct actagtataa taagtaatac tttgagtgga
     6961 aattatgtag aatatctgat aaacaatgac ataagtgcta aaatggctag tatattggta
     7021 agtttaatga atctaactta ttggtttgta atattctcaa taatatttgt atttataata
     7081 cttttaaaac atattttaaa tgttggaata aaagagtatt ttataaagaa tactgtcaca
     7141 ggaatgtttt ttgtatggtt gataaaatca ggaaaagtag ttattaatca aattagaata
     7201 acagatttaa aagaaaagcc taataaaact atagctataa ttttgggaat aaacttatta
     7261 attatagtta taatgtgtag tttttggttc tttggaataa tacttgcatt gatatattca
     7321 gtagtattat ttaagatatt gtcagattac tctaaaaaga caatatatga atataatcaa
     7381 cttttagatg ttacaaagaa aatttcagat ggaaacttag aagtaaatat ggaaagagat
     7441 ttaggatttt ttaatcctat aaaagatgaa ttaggaaata ttcaagctgg atttaaaaaa
     7501 gctgttgatg aagaggttaa gagtcaaaag atgaaaacgg agcttatatc aaacgtatca
     7561 catgacctaa aaacacctct gacatctata atcacttata tagatttatt aaaagatgaa
     7621 agtataacag atgaaaatcg taagttgtat attgatacat tagatagaaa gtcacaaaga
     7681 ttgcaacatc taatagaaga cttattcgaa gtaagtaagg taaatagtgg agatgttcat
     7741 ttgaacataa tgaatgtgga tattgtgtct ctaatgaaac aaacactttt agaattagat
     7801 gacaaacttg cagattcatc tctaatagta aagaataact tttctaatga aaaaataata
     7861 ttaccattag atagtcagcg tacatttaga gtatttgaaa atcttataat aaatataagc
     7921 aaatatgcaa tgccaaattc aagagtgtat attgatattt tagaaacaga caataaagta
     7981 agtattatgc ttaaaaacat gtctgcaact gagattgatt ttagtgtaga tgatataatg
     8041 gaaagatttg taagaggaga taagtcacgc aacacagaag gttctggtct tggtttagca
     8101 atagcaaaaa gttttataga attacagggt ggtaagatga atataagtgt ggatggtgat
     8161 ttatttaaag ttacaattac ttttaataaa aattagaata gacttcttta ataattataa
     8221 aatccataaa ataatatttt ataggtattg ttttaaagta gtttttaaac attatttcaa
     8281 aacaacttgt aaatcttatt ttaaaataga atgttgattt atttaaaaaa ggtgaatcct
     8341 aatatataag attcaccttt ttattttata aaatgttact tagtgttata gatattttaa
     8401 atagtgtaaa tttggattta ccattaacat aaggttagta atttcttttg aaatcttgta
     8461 taagctattt tatattattt aattactatt gtttgtactt tacaatgtga caaagaattt
     8521 acttctacag ttttagtatt taccttaagc ttgttagtat tatcatagat ttcgtcaagc
     8581 attgcattat aagcagcatt gatattttta ttgatgttaa gacttccttt agaatcctca
     8641 tttttcatac cattgaagat tctaactaca attccgtctc ctttttcttc tttctttatt
     8701 gcacttactg tgaaatttga attatttaca ttcataaaac tatattcatt ttcatatatt
     8761 tgtttttcat cattaaatac aaatataagt ctaccattta aaaaatcagc atattcatat
     8821 atttgtatag gtgttaagaa tctcttagca gtattagcta tatttgattt atcaaattta
     8881 ttgttgtaga tgtaaagacc aaagttaaag tttaactctt tgagtaattg tgcatctggt
     8941 gttgctatta ttttttctcc agaagctctt cctggtctat aaagtaaatt ttctcttccc
     9001 atatatccaa atgacctaaa tagtgttaat gctattgtat ctaaattagc accaactatt
     9061 tgatattctc tcacacaacc agttattaat gctactcctt tttgtccatc ttctaaagta
     9121 acaaaacttt gcattggttc aatagttata ggcttttctt gccatgagta attgtctctt
     9181 ttccataatg caaggtcttt ttctaatgct gttggtcttt gtattattcc aaattgttga
     9241 tctgctatag aaaacttaga tgctatttcc gtattaaata aaactcttac tctatgagat
     9301 aatgcttggt cattttttat attaatatta aacttaatta tttgctcttt aggttctaag
     9361 gtgattttca ttgttactgg cattttcaca gaaactttac ctatagtcct ttcttctagt
     9421 gaagctggta tggtcaatga atatttaagt gtcatttctt cttgaacatt tgattttagc
     9481 acttctactg aagatacaga atctaaagat gaaacataaa tatcttttct tggaggcgaa
     9541 tagttgtatg aatctccatc atctccatta tcttcaatta cagcttggtt tttataagtc
     9601 ttattagact ctttatctaa tatttctact gaaccatttt tattggctgt tattttatag
     9661 tatttgtttt ctattttttt acaactactt gtttttattt cacatttatt tttagtatct
     9721 aattcaacaa ttagctgagt atatcccatg ggtggtatat tttttactga aagtgcaatt
     9781 tttgccctat atacagtttt tggtatatat atttttttac ttgggtttaa ttttattgtt
     9841 tgagttaata tataatctgt aatatcttct ttttctttta ttatataatt aagaatttct
     9901 ccatttttgt tctttatcac aaaatcttga cctggaatat aagtatcaaa ttctacaact
     9961 tcatttctag cttctggtaa tccattgaat actgttaatg ttatttcttc attattattt
    10021 ttgatttgag tacttataag tctcatatga aaatctaaca aatttatact caaatctctg
    10081 gcttgtttgt atcttgtgta tacatcttca tttgttgtgt cactattaca agaacctatg
    10141 ctgtcatgag ctgcattttc gaacataagt ttccatatat cttttaaaat taggtgaggg
    10201 tattcatttc caagtgaata acttaatgtt aataatggtt caagtacatt tgtaatataa
    10261 ttttctaatt tattattcat tatcttaagg tctgaccttg aggagaatat tgatttgtgt
    10321 attctcatat gtttagcatt tacaaattca cctttaagct ctgtaaattc atttctttca
    10381 tttcttactg aatctacata gttttctata tcacttattt cgtattcatt ttcagtatct
    10441 attttattag ctttttttaa tatgtcagga aggttttttc ttattggtgc ttggtcaaat
    10501 ccatttggga aatatacgtt attagtagag gatttaacct taagttttcc aattgtatct
    10561 tttaaatatt cttttaaatc tgaatcatct tctggtatat tacctccata gtaataacca
    10621 aatggtattt gaactgctaa tacctttgag ccatcagaac ctttccatat aaattctgtt
    10681 gtatctacca tatcatcaga aactccacgc caaaataatg aactatctat tccaaaacct
    10741 ctatatattt gtggcatttg agctgattgt ccaaaagaat ctggtacata tccaaccctc
    10801 ataggttcgc caaattcatt gcatctatct attccatagt aaaggtttct aactatagat
    10861 tctcctgata taaccaattg gtctgtttga gtataccaag gtcctatagc taactttttt
    10921 ctttgaacta aatctcttat tctatattca tcctgaggtc tccattttaa ataatcatct
    10981 aagagagaag cttgtgcatc catagtaaag tatttaaagt cttctttttc ttctaatatg
    11041 tctaatatat cttgaaaatc tttcattaga tatatttttg accttgatgt tgtaaaatac
    11101 cattctctat cccaatgact atgtggaact acatgtactt ttgtcttctt cataaaatcc
    11161 tccctaaatt taattgagaa ttgagagtac gctcaattct caactagcta aaattaaatc
    11221 tttaatttac attatatcta actctatttc atcaaattct gtattttccg cagtttcatc
    11281 ttcattaaag tctactaaaa ttgttgataa tattgctatt actaaagctc caaatatcgc
    11341 acctactaag taagcccatc cagaacctac tgatataaat ccatataatc caccaactgg
    11401 aggcattata gcatgtacac ctaataaagc tgataatcct acacccaaag cacatcctat
    11461 catattgaca ggaactaatt ttaatggatt ttttaaagtg aaaggaattg caccttctgt
    11521 tattccaatt atacccataa ctatgttgcc ttttcctgtt tcagataatg tcttagagaa
    11581 tcttgatttt ctaatgaatg tagctaaacc atatcctagt ggagggataa ctattgcgca
    11641 gtttaccaat attgctggta gatagatgcc ctctaacaat aatgcatttc ctgccatcca
    11701 agctgcttta tttaccggtc ctcctaaatc aaatcctatc attgaaccta aaattaaagc
    11761 caataccacc gaatttgttc cattttgaca catttcagct acccaagcat ttaatgaagt
    11821 atttatgttt cctaaaggcc ctgctaatac aacactcatt aaaaaccccg ttaatccaac
    11881 tgttataagt ggcattatta taagtggcat cattcctgct gccgacccag atattttaat
    11941 ttttttcttt aaaaatttgc aaatatatcc agcaaaaaaa cctgctgtta ctgctcctaa
    12001 gaatcctgca ttagtattac ttgctatgag tccaccaata aaacctgcac ccatagatgg
    12061 tttttcacct attgaaaatg ctataaatcc tgctaataca gtatttaata gacctatgcc
    12121 agtccaacca acttgttcca ttaagtaaag aatatgataa aaaccggata catttttgta
    12181 agcatctaaa cttgttatac ccattgctaa agctgtaagt tttggtatag ctacaactag
    12241 agatgagcct attacaagtg gcaacatata tgaaatacct gtcattaagt gtcctttaag
    12301 ttcacttaaa agagagttat tttttttact catttttaat cctcctatat aagtttgtta
    12361 tattttttga taatatgtta taattacatt agataatttt ttaatttttg atttttctca
    12421 ttctagatta gagtgagatt ctttttatta agaaatagtt tctatacact ttgttaagac
    12481 agcttctggt ttttttaatg ctacacctat tttaactttt attacttttt taccatcaaa
    12541 tctttccatt ccagatatag tttggtcaga tgctataagt ataaagtctg catcttgtgc
    12601 ttcttctttt gttatagtat ttactcgtcc catagcacct tgttgttcta ctttgatttt
    12661 atgccccatt tttgatgcag cttgttccaa agcttttgct gccataggtg tgtgtgccaa
    12721 tcctgctata catgatgtta ttgctactat tttcataatt attcctcctt cattttatta
    12781 gttatatatt ttactaaatg acttgcatca ctagcatttt taacttcact tataaaatca
    12841 tcttccatta agcatgtggc taatttagat agagttttca aatgtatatt tccttcattt
    12901 tcatgtggag ctaacaaact aaatacatat ctaaccttac tatcatctaa agttttccac
    12961 tctaactcgt tagttgtttt aatatagagt atagaagctt cttttacatt tgcacttttt
    13021 gtatgaggta tcgcaaatcc atctcttatt cctgttggaa attcgctttc tctttttaga
    13081 aggtctttta aaagagcatc tttattagtt gtaataccta gtatataagc actgtctgca
    13141 ataaaattta atacttcctc tttgcttttt aagtctttat ctataaatat attcttttct
    13201 tgtagtacca tatatttttt ccactccatt cgtaatgtct taacttatat ttatattgta
    13261 atcgttatcc taattaaatt aaatataact aatttccact tcgacatgga aatttatatt
    13321 atgataataa ctaatttcca tgtcgaagtg gaaataaatt atgataatac aattttatta
    13381 gagtaattgt attatatgag tgtaaatagt ataatttaga agaaattcta taagggggga
    13441 tatttatgca caagagattg ctcacgttat ttaaactatt aaatgaaagt gatgataaaa
    13501 ttacttgtaa aactttaagt aatcatttaa aagtgagtga gagaactata agaaatgata
    13561 ttacttctat aaatgaaact ttagagaaaa atggtgctat tattaaaata aaaaaaggag
    13621 aaggatatta tatagatatt cttaatttag cattatacca acattatcta gcattaatat
    13681 ctgatgatat tatggattca agtgaaatac cagattctcc tatagaaaga aatcaatata
    13741 ttttaaaata tattctttat aacaatacat atataaaact agaagattta gctaatagct
    13801 tgtatgtgag taaatttaca atactaaatg atataaaaag aataaagcct attttgtcaa
    13861 agtataattt aatattagtt tctaagccat attatggagt taaggttgaa gggaaggaaa
    13921 tagatattcg aagatgtatt tctaataata tgataaatag gaattttgaa aattatataa
    13981 ttggaattac agatagagaa atagaattat ttaataatgt agatttaata gagcttcaga
    14041 agatagtgct aagtgaaatt aataaattta atatcaattt cttagatttt aatctaaaaa
    14101 attttattat tcatttatct attactataa gcagaatact aggtggttac tgcttagata
    14161 atgttttaga tgtggtttta acagattttc aatcgaatac ggctgttgaa aatatattta
    14221 attatattga gagtaaatat actataatta tttcaaaggc agacagagtt tatttatata
    14281 atcattttat aactaaaagt tctcttttgg ataacgtttc caatagggca gataccaaaa
    14341 ttatagagta tgttgaggaa attttagaag taatcaacaa tcaatatact tttgacctta
    14401 gaaatgatag tgtattattt gatgatttag tactgcactt taaatcaatt cttaattcta
    14461 aatcttataa tttaaataag gtgaatcccc ttataaatac aataaaaagt aattatcctt
    14521 tagcatttga aataagttta aatgctatag aaaaagtttt taaaaatagt atttactctt
    14581 taacagaaga tgaaattgga tatgtatccc tacatatagg tgctggaata gagagatttt
    14641 ttcaaaacaa tattaagtgt aaaaacgtag tattagtatg tggttcaggg tatggttcaa
    14701 gtaggttatt agaggttcaa ttaaacaaag tttttcatga taaaataaat atacttcaat
    14761 gtctatcatt taatcaattt ttagctagtg aattgtcaga tgtagatata ataatatcga
    14821 caattccttt aaatcatgat agtattccta ttgtattagt agatttaaaa ctattaaaaa
    14881 aagatataga gaatatatct aagtcaatca ctaataattc tcgtatttat agtaatcttt
    14941 tggacaattt ctttgataaa aatttattta ttgttaatcc taaaatcaaa gagaaggatg
    15001 agcttataaa gttgatgtgc aacaaattaa ctcaaagtga aattgtaatt ccatcattta
    15061 ctgaatctgt attctacaga gaaagtttat cttctaccaa tatagatgat tttttagcta
    15121 tacctcatcc tatggaatta agctctataa aaactaagat ttgtatttcc atattgaatg
    15181 aaccaatata ttggagtgaa gattctactg ttaaattaat catgatgttg gctataaata
    15241 aagatgatta tataaaaata aactccattt atgatatcct tttaaagata attcatgaca
    15301 atgatattag agatagtata tctaattgta atgattttga tagttttctt tccataatca
    15361 agtctataat ataaaatttt atattacata agttttataa aaaataaata gaacaatttt
    15421 ttgttgataa tataaatagt aatgagtaat ttttatatta ttttctatta ctttatactg
    15481 attgtaatta gatacaatag agggtctgtc tcaaaataga gataatcctt tttttattgt
    15541 tgaaatgata aaaacatatt tgatttcata taaaaaagag tgtctcatga actaaaaagt
    15601 tcattttgag a
//
LOCUS       DAMJVM010000239        11087 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT2022 isolate JH278
            SAMN32366131-rid16589983.guided.115, whole genome shotgun sequence.
ACCESSION   DAMJVM010000239 DAMJVM010000000
VERSION     DAMJVM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366131
            Sequence Read Archive: SRR22863030
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560886.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560886.1
            Genome Coverage       :: 242x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,630
            CDSs (total)                      :: 3,578
            Genes (coding)                    :: 3,502
            CDSs (with protein)               :: 3,502
            Genes (RNA)                       :: 52
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 28 of 76
            Pseudo Genes (incomplete)         :: 56 of 76
            Pseudo Genes (internal stop)      :: 23 of 76
            Pseudo Genes (multiple problems)  :: 26 of 76
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11087
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366131-rid16589983.guided.115"
                     /strain="MT2022"
                     /isolate="JH278"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2013-06"
     gene            125..1531
                     /locus_tag="PC523_003619"
     CDS             125..1531
                     /locus_tag="PC523_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2477068.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYNNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGIDFYNKII
                     DKLIECDIEPFVTLYHFDLPFTLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMRYLEKRNIVPHM
                     EDGDKEVLKKAKMDFIAVNYYRTNCAGAFPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACRLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            1559..2407
                     /locus_tag="PC523_003620"
     CDS             1559..2407
                     /locus_tag="PC523_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2477069.1"
                     /translation="MYITKVLNNSLLLARDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLELPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSKEKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            3067..3759
                     /locus_tag="PC523_003621"
     CDS             3067..3759
                     /locus_tag="PC523_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2477070.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEHIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKNDNYSKVHGLRAGADDYLTKP
                     FEIAEFTARVLSLIRRYTRFNPKNEESQQLSFKGLLIDLDQHTVTTNGETMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            3756..4691
                     /locus_tag="PC523_003622"
     CDS             3756..4691
                     /locus_tag="PC523_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2477071.1"
                     /translation="MKMDEQTIILFLFLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGNENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIGQEREEYIEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLDFEIDIPENRLMVNLDSDGYSRVVNNLVQN
                     VLAHSKARRIKITMSEDSKMVLLRVEDNGVGIAKENLQHIFERLYKCDKGRSKKGSGL
                     GLSIVSQMVERMGGKISVESEIGKYTVFIVSLPLI"
     gene            4841..5764
                     /locus_tag="PC523_003623"
     CDS             4841..5764
                     /locus_tag="PC523_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2477072.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGSGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRKFIRNLCTERGKTILISSHILSEIALLADDIGIIDRGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLEIQLNLKNFKVADNHNIMLYDTDVSVAEINRAF
                     IQNNIDVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            5748..6518
                     /locus_tag="PC523_003624"
     CDS             5748..6518
                     /locus_tag="PC523_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2477073.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIVFGIMFFVTALPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICIIYKKQEN"
     gene            6528..7310
                     /locus_tag="PC523_003625"
     CDS             6528..7310
                     /locus_tag="PC523_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2477074.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQIVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFVITLIGGYLINREYTDQTLKNILTIPISLRKLLVGKL
                     IAVGGITVLLSIFSFLCTFILGLLFCNINISVILVAKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNSFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLAGYNNDDGSVYQPLI
                     GVMTLVVILILTAILLTFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            7688..8224
                     /locus_tag="PC523_003626"
     CDS             7688..8224
                     /locus_tag="PC523_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2477075.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            8256..8645
                     /locus_tag="PC523_003627"
     CDS             8256..8645
                     /locus_tag="PC523_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2477076.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLSQLTEHGFIKKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLKELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            8682..9068
                     /locus_tag="PC523_003628"
     CDS             8682..9068
                     /locus_tag="PC523_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HDF2477077.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNSVTVKVSSAIHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            9195..10637
                     /locus_tag="PC523_003629"
     CDS             9195..10637
                     /locus_tag="PC523_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2477078.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDENIAEDIVEDEPKLNTEKDGDLNYVSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAKSKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGKDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            10934..11053
                     /locus_tag="PC523_003630"
                     /pseudo
     CDS             10934..11053
                     /locus_tag="PC523_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
BASE COUNT         4001 a         1393 c         2092 g         3601 t
ORIGIN      
        1 aaagaaaata gttaatataa agtgaactta ttgtaatgaa ttattaaaga aaataattaa
       61 tatagattaa acttatttta atgaagtaca aagaaaataa tcaatataaa aaggagaaat
      121 atttatgaat acaggatttc caaaagattt tttatgggga gcatcatctt ctgcatttca
      181 agtggaagga gcttgggata aagataataa aggaaaaacc gtagctgact ataattcttt
      241 taaaaagtct catttacagg cagatactaa agtggcatca gacttttaca ataattatga
      301 agaagatatt gaattaatga aagaattagg gatgaaaaca tatcgttttt caatttcatg
      361 ggcaagaata ataccagatg gtgaaggaga gataaaccag aaaggtatag atttttataa
      421 taagataata gacaaactaa tagagtgtga tattgaacca ttcgtaactt tataccattt
      481 tgaccttcca tttacattag tagagaaata taatggctgg gaaagtagag aaactgtcta
      541 tgcttttgaa agatttgcta aaatatgctt taagcatttt ggagatagag taaagtattg
      601 gcaacctcat aatgaacaga atttaattgt tagagtggaa gagagaatta atatatatga
      661 tgaaacagat tcatggaaaa tagataaaat gcgtgctcaa atggactata atctttgttt
      721 agcacatgca ttagcagtca atgcatgcca tgagatgata aaagaaagta aaattggagc
      781 agcagtatca tcatctgtaa catatcctct tacttccaag ccagaagatg tatatgcagc
      841 tagaatgaat gataatttta aagtgtacta tatgcttgat atgcatcact atggagaata
      901 tccagggtat tatatgaggt atcttgaaaa aagaaatata gtccctcata tggaagatgg
      961 ggataaagaa gtacttaaaa aggctaaaat ggattttata gctgtaaatt attatagaac
     1021 aaattgtgca ggagccttcc cagaggattc acaacatcca tttgggttaa gagaaggaac
     1081 tgttgatttt agtatgtatg gattatttaa aatgtcaatg aatcctaacc tagaagcttc
     1141 tgagtatgga gcagcaatag acccatcagg tcttagagtt gccttaaatg aatattggca
     1201 aagatatcac ttacctgtta taataacaga aaatggtctt ggagctaaag acatattaga
     1261 agatggaaaa attcatgatg actatcgtat agattattta aggagtcata taaatgcatg
     1321 tagactagct attgaagatg gtgtagagat gattggatat tgtccatggt catttacaga
     1381 ccttttaagc tcttctcaag gttttaataa gcgttatgga cttgtttata taaatagaac
     1441 tgaccatgaa gttttagatt tgaaaagaat caagaaagat agtttttact ggtacaaaga
     1501 agttatagag aacaatggaa tagtaaaata aacataaata tatattgagg gttttagtat
     1561 gtacattaca aaggtattaa ataattcgtt gctacttgca agggatgata atgatgaaga
     1621 aataatattg atgggaaaag ggattggcca taattataag gctgggtctg agttaaataa
     1681 aaaagatata gaaaagattt ttgttcttca tgatgaaaat ataaaaaaga gtattataca
     1741 actggcaaca gaaatagatg cagaatattt tggtatagct caaatgataa ttgcatatgg
     1801 aatagaaaaa tataacttaa aacttatgaa ccatatatat ttggccttaa ctgaccacat
     1861 tgcctttgca gtgagacgct ttaaagaagg tataatgatt gaaaatcact atttgtttga
     1921 aatcaaggag tttaatccaa aagagtatga tataggaaaa tatgcaatta ctgtttttaa
     1981 ggaggttctt ggtttagaac taccagaaga agagattggt aatatagcag gccattttat
     2041 aaatgcacaa caagataatc cttatagtga tagaaataag agaagtgcta aaattgtaaa
     2101 tgctattttg caaatagttc attatcactt ttctattgta tataataaag agtcatttta
     2161 ctataggaga tttgtaatgc atctaaaggc cttttcacaa agatttctgg ctaacgaacc
     2221 gtcaaaagaa aaaatagatt ttatatatga gcaagttcaa aaaaattgta aggaagagta
     2281 tgaatgtgtg aaaaaggtag gagcttatat gtataaggaa tactccagag aattaccaag
     2341 acaagaggag ttatatttga tgattcatat tcataagatt ttaggagaat tatgcgaaaa
     2401 tagttagaat atcttaatat gctagtaata ttataaaaaa ttaaaaatta attacacttt
     2461 aaatatgaat aattgcatta cttaaggggg ttgtcttaaa attgattttt ttaattaaat
     2521 tttaagttca actccttttt aaacctttat atcattacaa ctttaatgtt tatacgcctg
     2581 tacttcagat gtgtatctat tttttacatt tctcaacatg aaaatttatc attttttgta
     2641 gtgtgttaat tgaaataaac ctttgagtta accctaaatt tattctggac ctacaggaat
     2701 agttccaaag atactttaaa atattttctg gaatgattgc ttatttcata tatattttat
     2761 tgtgatgttt tgatttaata aattcttcaa ctccgtatat agtaaatgta ttatcatgtt
     2821 tgttttattt aagcacactt ataatatgct tgattttttc aaaatgatga aatatactaa
     2881 tgggtgttgg atgtataaga atcacattat acttgaaaaa gtaagtgaga aatgaggtat
     2941 aattcctcat atgataaact actattgtat tgtggaacat attctcagtg atgtcaagat
     3001 attacaatat gactaaattc ctaatgataa aaaagtctac gaatgagtgt agaaagggga
     3061 actaagatga ataaagtttt aattatagat gatgataaag atctttgtat tttgttaaaa
     3121 aagagtatct caattgaaca tattgaagca aactattgcc attctgggag agatggattg
     3181 gttgaacttt caaaacaaga ttatcagtta gtagttctag acgtgatgat gccaggtatg
     3241 gatggttttg aaactatgga acaaatacga aaatatagca gtattcccat cttaatgctg
     3301 acatcgaaaa atgataatta cagcaaagtt catggattgc gagctggtgc ggatgactat
     3361 ctaaccaaac catttgaaat tgcagaattt actgctcgtg tactgtcttt aatccgacgt
     3421 tacacccgtt ttaatccaaa gaatgaggag tcacagcaac tttctttcaa aggtctgttg
     3481 attgaccttg accagcatac agtgactacc aatggcgaaa ctatgttgct tccacccaag
     3541 gaatttggtg tgcttcttta ctgtgcaagg catcagggta agattttgac aaaacagcag
     3601 atttatgaag ctgtgtgggg tgagccatat gtatatgatg acagcaatat catggctatt
     3661 atcagtcgcc taagaaaaaa aattgaacta gacccatcat ctcccatcta tttgcaaacg
     3721 ataaaaggaa tcgggtatca attcaacacg gaggtatgaa aatggatgaa caaacgataa
     3781 ttcttttttt gtttttgctg tcgattggtc tacttttcct ttccatattt gctttttcca
     3841 agcttggtca tattcacaaa cgtctaaaag atatagaaga aatacttgct gatgtacaaa
     3901 ttggaaacga aaatcgaaaa atactaatta agccatgtga tgttatggct ccattggttt
     3961 atcagcttaa tgagattgtc tacgattatg aaaataagtt actttctctg aaaaagtcgg
     4021 ataaagctag caaacaactt atgacaagtt tatctcatga tgttcgtaca ccattaacga
     4081 ctctgattgg ttatctggat gctgtacaca gtggtatagt tatcggacaa gaacgggagg
     4141 aatacattga aatcgctcgg cgtagagcct acgaccttaa ggattatatc gatgttcttt
     4201 ttgactggtt tcgattgaac tctgatgaat ttacgttgtc aatagaatct gtggaaattg
     4261 cagagttgtc acgtaatatt cttaaagatt ggattcctat cttccatgaa aagaaattgg
     4321 attttgaaat tgatattcca gaaaatcgac taatggtaaa ccttgattca gatggatatt
     4381 ctcgtgtagt caataatcta gttcaaaacg ttcttgccca tagcaaggct cgccgaatca
     4441 aaattaccat gagtgaggat tctaagatgg tattacttcg tgttgaggat aatggtgttg
     4501 ggattgccaa agaaaattta cagcatattt ttgagcgact gtacaagtgt gataaaggac
     4561 gttcaaaaaa aggaagtggt cttggtttat ccattgtcag tcagatggtg gagcgaatgg
     4621 gcggtaagat ttctgtagaa agtgaaattg gaaagtatac cgtatttata gtgagcttac
     4681 cattaatata aaaattgttt ttcccccatg tgatatatat acacaggggg gattttttat
     4741 aagctataaa gttgcaaggt taatacaagg ttgttgcaag gttaatgtaa gattgaggtg
     4801 gtatgataaa tttaataaac aaaggaggat tttactattt atgaataatt taattattga
     4861 aacaaaaaat ctcactaaac aatatggtgc tcaaaagagc gtttctaatt tgaatattca
     4921 tgttcagaaa ggtcgtattt atggattgct gggaagaaat ggttcgggta agacgacaac
     4981 tatgaaaatg ttattgggtc taacaaaacc aactactgga gaaattaaaa tatttggtca
     5041 ggatattcat aataacgaga aaaagcttct tcctagaatt ggatgtttga ttgaatcacc
     5101 aggtttttat cctaatctga caggaacaga aaatctcaaa atctttgcga ggcttagagg
     5161 aataccaaaa aaaaatgcag tagaatcagc actggatgta gtcggtttgc caaacaatga
     5221 caaaaagtta ttttctcaat actctcttgg tatgaaacag aggttggcaa ttgctcttgc
     5281 cattatgcat gacccacaat tgttgatttt ggacgagcct atcaatggac ttgatcctat
     5341 tggtattgca gaagtaagaa agtttattcg taatctctgc acagaacgag gtaaaactat
     5401 tttgatttcc agtcacattc tttctgaaat tgctttgttg gcagatgaca ttggcattat
     5461 tgaccgtggc attttgttgg aggaagaaag ccttgcacaa ctggaagaaa aaaacagtaa
     5521 atacatacat tttgctgtat ctgatgcagc acaagcagca agacttctgg aaatccagct
     5581 taacctgaaa aatttcaaag tggcagacaa tcacaatatt atgctttatg atacagatgt
     5641 ctctgttgca gaaataaatc gtgctttcat tcaaaacaat attgatgtat cagaatctca
     5701 tttgtgcgaa gatactcttg aagattactt caagaaagta acagggggtg aaggaattgc
     5761 ttgatataat ttcatgcgaa ttttcaaaac tcaagcggtt aaaatttata ctgatttcta
     5821 ttttaggtgc ttgtttattt ccaattcccg caacaatttt gattgcaaag gacaacttgc
     5881 cttttgagca attgttcaaa ttagttgtca attttggata ttttttgcta ttaccaattg
     5941 tattgagtat tgttgctagc caactctttt tcatagaaag agataacgat gtattaaaaa
     6001 atctagcaac agtgccagtg ccaaaaggaa aactggctct ggcaaaatta gctgtgttgc
     6061 tgtttatatc attgttctac tccgttgcag ggttaggagc tactatcata ggaggtttca
     6121 ttgttggaat agtagagggg gttgctgtaa aattaggaat gagtattgtt tttggaatta
     6181 tgttttttgt tactgcactt cctgttgtgg tactcattgt atatttcaat cgcagctata
     6241 ttttttctat tatactttct tttgtatatg ctattttcaa ttttagtata tccttaaata
     6301 ttatcaactt tgaaccaaat aacccactgt taagcgtatt acctgctcct gttataatgc
     6361 gttggtggat ggcattttgg ggagatccca ctggcgaata cacggcactt agacagccat
     6421 atttactctc aactcctgct tgtgtaggta ttttatgttt gataacaatt attgcagtcc
     6481 tgttgatttg tataatctac aagaagcagg agaattagga ggtatctatg cttgatataa
     6541 ttactacaga atttcaaaaa ataaaaagat ataatattct atggattggt gttgttgctg
     6601 ttctgttttc agcactattg gcggtttttc agattgtaag ttcacacggc agtgaccctc
     6661 taacgtatga gagctttgca aatggagtta tatggaataa tttcagtctt agttttccat
     6721 ttgtgattac gctcattgga ggatatctta ttaaccgtga atatacagac caaaccttaa
     6781 aaaacatcct aactattcct atttcattac gcaaattgct agtgggaaaa ttaattgcgg
     6841 ttggaggaat tactgtattg ttaagtattt tcagcttctt atgcacattt attttagggt
     6901 tactattttg caatatcaat atatctgtta tactcgttgc aaaatcctta gttcagattt
     6961 tgtctgttaa tctgtattgt tatgttgctg ttttgccgat tattgcttat tttggtcgta
     7021 aacaaaattc ctttttgaca ggcgtaggga ttgcatttgt atatggcttc tgtggtgtat
     7081 ttgttgctgg aagaaatcta acagactttt atccaatcac agcaggtctt ggtctagctg
     7141 gatacaacaa tgatgatggt agtgtttatc agccacttat aggtgttatg actttggttg
     7201 taattctaat cctgacagct attctactta catttacacc taattacgat aaagttatga
     7261 ccatatctaa gaaaaaaggg ataaagcata aaaagtattc tcataaataa cataaatatt
     7321 gtttctatta tttaagttgt caggcagtga aaagaaaaaa tctgtcgtac agtggctata
     7381 tgctcatgta tttaagaagc tacagttttg atttttcata ggtgtggctt ctttttgtat
     7441 ttattaaatc aacaactatc ataatatttg caataggtat agtaacaaga gataatactg
     7501 tcatatgtgt tttatatatt acattaagta tattctaaaa tatatgtttt aaataatata
     7561 tttgaaaata agaatacttg aataaaaaaa ataatattat atatagttga caaaaagtgg
     7621 aaattaatat actatataaa tatagaaata gtaatcatta ctaatattaa aaaaggagga
     7681 attaattatg aacttaaaag gaactaaaac agaaaagaat ttaatggcag gatttgcagg
     7741 agagtcagaa gcaagaaata aatatacata ctacgcttca aaagctagaa aagaaggtta
     7801 taatcaaata gctgctatat ttgaagaaac tgctaacaat gaaaaggaac atgctaaatt
     7861 atggtttaaa cttttacatg atggtatgcc ttcaacagag gaaaatttaa aggatgcagc
     7921 agcaggcgaa aactatgagt ggactgacat gtatgcgaaa tttgctaaag aagctagaga
     7981 agaaggattt gacaagatag cttatttatt tgaggctgtt ggaaaaatag agaaagagca
     8041 tgaagaaaga tacttaaagc ttttagaaaa cttaaatgaa ggaaaaatat ttaaaagaga
     8101 tgaagaagtt gtttggcaat gtcaaaactg tggtcatgtg tatgtaggta ctgaagcacc
     8161 agaaaaatgt ccagtatgtg accatccaaa agcatatttc aatataaaag ctgaaaatta
     8221 ttaaaaatta tcattattaa aaaggaatgt taactatgaa attttctaaa caacgagaac
     8281 tgattttgaa tgaaatatta aataatccgg ttcatcctac tgcggattac ctatatgaaa
     8341 acttaaaaaa agataatcca aatctaagct taggaactgt gtatagaaat ttatctcaac
     8401 taacggaaca tggctttata aaaaaggtta gtattccagg gtatccagat agatttgatg
     8461 gtagaataga taatcactat catatcatat gtgaagtatg tggagaggta tacgatttag
     8521 aatctgaagt tcttaataat ttaaaagaat taatatctga ggagacagac ataaaaataa
     8581 catcttataa cataagtttt aaagggattt gtaataattg taaaagatgt agccaagtag
     8641 gttagctttt aaattctaaa aataattaat gaggtgagtt tatgtgtagc gaacaaaaat
     8701 tttttatatg tgagacttgt ggaaatcttg taggaatgat acaaagtggt ggtgtaccta
     8761 ttttctgttg tggaaaacct atgaaggagc ttgttcctaa tacaacagat gcagcagttg
     8821 aaaaacatgt acctgtgatt gaagtggatg gaaatagtgt tactgttaaa gtaagtagtg
     8881 caattcatcc aatgactaaa gaacatcata tagcatgggt gtatcttatg actgaacaag
     8941 gtggtcaacg caaatgtcta gcagtagatg gagaacctgt agttaaattt gctctgaatg
     9001 atgatgataa agtgatttct gcatatgctt attgtaattt acatggttta tggaaggctg
     9061 aactgtaata tttaaataaa ttgagtcaaa aaggaggcta taaatgtatt ttcatacaaa
     9121 tagcctccta taaatttata taaattcgag ttttaatttt atttaatgtc aatataatat
     9181 aaggagaaaa taatatgtca atttacaaat gtagcgtttg cggatatatt tatgatgaat
     9241 ctaagaatga taaaacatgg gatgaattaa gtgaagactg ggaatgccct gtatgtacaa
     9301 aaggtcgtag ttattttgga aagataagta ctgtttacta tgaagaagat gaaaatatag
     9361 cagaagacat agttgaagat gaacctaaat taaatactga aaaagatgga gatttaaatt
     9421 acgtaagtac atatttaaga agagatgatg aagttgagaa acatatggat ataattcacg
     9481 aaatggctgt tacaggaaaa tctataatag aaccgatgag aactaaacta ccagtaattt
     9541 cttgggatga tattttaatt atgggtgctc aattaaatcc attaccttta aacgaacatg
     9601 atgaagtaaa tacaacaaca ataataggga aaaaagcaaa gaagcctatg attatagaaa
     9661 atccagttta tatttcccac atgtcatttg gagctttatc taaagaacta aaaatagctt
     9721 tagcaaaggg tgccgctaaa agtaagacag ctatgtgtag tggagaaggt ggtatattac
     9781 cagaagaaaa agaagcttca tataagtata tttttgaata tgtaccaaat aaatatagtg
     9841 taactgaaga aaatcttaaa aattctgatg ctatagaaat aaaaattggt caaggaacaa
     9901 aacctggtat gggaggtcat cttcctggtg aaaaagtaac tgaagaaata gcaaaggtaa
     9961 gaaataagcc tgtaggtaaa gatgtaataa gtccatcttg ttttgaggaa atacaatcca
    10021 aagaagattt aaagaaactt gtagatgaac ttagagaagt ttcagaaggt cgtccaatag
    10081 gtgttaaaat ttcagctggt catattgaaa aagatatgga atttatagct tatgctaaac
    10141 ctgattttgt gactattgat ggtcgtggtg gagctactgg agcaagccca aagttgctta
    10201 aagatgcaac ttcaatccca acaatatttg cactttatcg tgctagaaaa tacatagata
    10261 cacatggatt agatatagat ttggttataa caggtgggct tcgtatatct actgattttg
    10321 ccaaggcaat tgctatgggt gctgatgcag ttgctattgc aagttctgct cttatggcag
    10381 cagcatgtca acaatatcgt atatgtggtt ctggtaagtg ccctgtagga gtagctactc
    10441 aggatgagga attgagaaaa agattacata tagaaaatag tgctaataga gtggcaaact
    10501 ttttaaatgt aagtttagaa gaacttaaga cttttgctag aatttctggt cataaagata
    10561 tacatgattt aagtgttgat gatttatata ctgttaactc tgaaatatca aattacacaa
    10621 acatacaaca tgtataagtt gaagtttgag actattagat tgtagatagt tataaaagta
    10681 aatgataaat atattttata aaaaggagtg tcttaaaatg aactttttag tttataagtc
    10741 actctttttt gtatgaagcc aaatatattt atcattttaa caatgaaaaa gaatcttata
    10801 tctttacttt gagatagatt ccttctattt atgaaaatta aagatattaa attgtaaaac
    10861 attctgtgat gtcgtactat taaactaaag atgctgtact ttaaagtaaa taaaaattta
    10921 aggagtgatt attatggaaa aattgaagat tcaagttcta gttgacaata atacatatat
    10981 tgataggtac tttgttggag aaccagcagt aagctattac attgagattg atggaaatag
    11041 gattttgttt gattaaaaaa tagctacgaa cacttgtgac tttagtc
//
